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Sample records for gef efficient industrial

  1. Brief introduction of GEF efficient industrial boiler project in China

    SciTech Connect

    Meijian, T.

    1996-12-31

    The present situation of installed industrial boilers, their efficiency and environmental impact are assessed. And the factors contribute to the low efficiency and serious pollution are summarized. Based on WB-assisted GEF project, {open_quotes}Efficient Industrial Boiler Project{close_quotes} aimed at CO{sub 2} mitigation in China, a series of effective measures to bring the GHG emission under control are addressed, in technology, system performance, and operation management aspects.

  2. Industrial Efficiency and Fatigue

    PubMed Central

    Crowden, G. P.

    1930-01-01

    The problems of industrial efficiency and fatigue offer increasing scope for the use of that special knowledge of human life with which medical men are equipped by their training. Success and prosperity of industry depend as much on health and efficiency of workers as on the efficiency of machines. Impetus given by European War to study of this human factor; national necessity led to establishment of Health of Munition Workers Committee which later developed into the Industrial Fatigue Research—now the Industrial Health Research—Board of the Medical Research Council. In Germany extensive investigations are now pursued at the Kaiser-Wilhelm Institut für Arbeitsphysiologie, at Dortmund, into such problems as relationship of age to capacity for heavy muscular work, influence of diet and nutrition on human efficiency, and optimum height of stairs up which loads have to be carried; new system of training apprentices developed in Germany since the war. Factors influencing efficiency and capacity for work of employees may be placed in two general groups, intra-factory conditions, and extra-factory conditions. Many of these factors have been investigated in this country and in America. In particular, the effect of the environmental conditions of temperature, humidity and air-movement on human efficiency has been studied: but much remains to be done. PMID:19987375

  3. Industrial energy efficiency policy in China

    SciTech Connect

    Price, Lynn; Worrell, Ernst; Sinton, Jonathan; Yun, Jiang

    2001-05-01

    Chinese industrial sector energy-efficiency policy has gone through a number of distinct phases since the founding of the People s Republic in 1949. An initial period of energy supply growth in the 1950s, 1960s, and 1970s was followed by implementation of significant energy efficiency programs in the 1980s. Many of these programs were dismantled in the 1990s during the continuing move towards a market-based economy. In an effort to once again strengthen energy efficiency, the Chinese government passes the Energy Conservation Law in 1997 which provides broad guidance for the establishment of energy efficiency policies. Article 20 of the Energy Conservation Law requires substantial improvement in industrial energy efficiency in the key energy-consuming industrial facilities in China. This portion of the Law declares that ''the State will enhance energy conservation management in key energy consuming entities.'' In 1999, the industrial sector consumed nearly 30 EJ, or 76 percent of China's primary energy. Even though primary energy consumption has dropped dramatically in recent years, due mostly to a decline in coal consumption, the Chinese government is still actively developing an overall policy for energy efficiency in the industrial sector modeled after policies in a number of industrialized countries. This paper will describe recent Chinese government activities to develop industrial sector energy-efficiency targets as a ''market-based'' mechanism for improving the energy efficiency of key industrial facilities.

  4. Industrial Energy Efficiency and Climate Change Mitigation

    SciTech Connect

    Worrell, Ernst; Bernstein, Lenny; Roy, Joyashree; Price, Lynn; de la Rue du Can, Stephane; Harnisch, Jochen

    2009-02-02

    Industry contributes directly and indirectly (through consumed electricity) about 37% of the global greenhouse gas emissions, of which over 80% is from energy use. Total energy-related emissions, which were 9.9 GtCO2 in 2004, have grown by 65% since 1971. Even so, industry has almost continuously improved its energy efficiency over the past decades. In the near future, energy efficiency is potentially the most important and cost-effective means for mitigating greenhouse gas emissions from industry. This paper discusses the potential contribution of industrial energy efficiency technologies and policies to reduce energy use and greenhouse gas emissions to 2030.

  5. California Industrial Energy Efficiency Potential

    SciTech Connect

    Coito, Fred; Worrell, Ernst; Price, Lynn; Masanet, Eric; RafaelFriedmann; Rufo, Mike

    2005-06-01

    This paper presents an overview of the modeling approach andhighlights key findings of a California industrial energy efficiencypotential study. In addition to providing estimates of technical andeconomic potential, the study examines achievable program potential undervarious program-funding scenarios. The focus is on electricity andnatural gas savings for manufacturing in the service territories ofCalifornia's investor-owned utilities (IOUs). The assessment is conductedby industry type and by end use. Both crosscutting technologies andindustry-specific process measures are examined. Measure penetration intothe marketplace is modeled as a function of customer awareness, measurecost effectiveness, and perceived market barriers. Data for the studycomes from a variety of sources, including: utility billing records, theEnergy Information Association (EIA) Manufacturing Energy ConsumptionSurvey (MECS), state-sponsored avoided cost studies, energy efficiencyprogram filings, and technology savings and cost data developed throughLawrence Berkeley National Laboratory (LBNL). The study identifies 1,706GWh and 47 Mth (million therms) per year of achievable potential over thenext twelve years under recent levels of program expenditures, accountingfor 5.2 percent of industrial electricity consumption and 1.3 percent ofindustrial natural gas consumption. These estimates grow to 2,748 GWh and192 Mth per year if all cost-effective and achievable opportunities arepursued. Key industrial electricity end uses, in terms of energy savingspotential, include compressed air and pumping systems that combine toaccount for about half of the total achievable potential estimates. Fornatural gas, savings are concentrated in the boiler and process heatingend uses, accounting for over 99 percent to total achievablepotential.

  6. Energy efficient industrialized housing research program

    SciTech Connect

    Berg, R.; Brown, G.Z.; Finrow, J.; Kellett, R.; Mc Donald, M.; McGinn, B.; Ryan, P.; Sekiguchi, T. . Center for Housing Innovation); Chandra, S.; Elshennawy, A.K.; Fairey, P.; Harrison, J.; Maxwell, L.; Roland, J.; Swart, W. )

    1989-01-01

    This is the second volume of a two volume report on energy efficient industrialized housing. Volume II contains support documentation for Volume I. The following items are included: individual trip reports; software bibliography; industry contacts in the US, Denmark, and Japan; Cost comparison of industrialized housing in the US and Denmark; draft of the final report on the systems analysis for Fleetwood Mobile Home Manufacturers. (SM)

  7. PRODUCTIVITY BENEFITS OF INDUSTRIAL ENERGY EFFICIENCY MEASURES

    EPA Science Inventory

    A journal article by: Ernst Worrell1, John A. Laitner, Michael Ruth, and Hodayah Finman Abstract: We review the relationship between energy efficiency improvement measures and productivity in industry. We review over 70 industrial case studies from widely available published dat...

  8. Recovering waste industrial heat efficiently

    SciTech Connect

    Hnat, J.G.; Bartone, L.M.; Cutting, J.C.; Patten, J.S.

    1983-03-01

    Organic Rankine Cycles (ORC's) are being used in the generation of electrical or mechanical power in situations where little demand exists for process steam. Using organic fluids in Rankine cycles improves the potential for economic recovery of waste heat. The right organic fluid can enhance the conversion efficiency by tailoring the ORC heat recovery cycle to the thermodynamic characteristics of the waste heat stream. The selection of the working fluid is affected by its flammability, toxicity, environmental impact, materials compatibility, and cost. Water, ethanol, 2-methyl Pyridine/H2O, Flourinol, Toluene, Freon R-11, and Freon R-113 are compared. An organic cycle using toluene as the working fluid is schematicized.

  9. The energy efficient industrialized housing research program

    SciTech Connect

    Brown, G.Z.

    1990-01-01

    The United states housing industry is undergoing a metamorphosis from hand built to factory built products. Virtually all new housing incorporates manufactured components; indeed, an increasing percentage is totally assembled in a factory. The factory-built process offers the promise of houses that are more energy efficient, of higher quality, and less costly. To ensure that this promise can be met, the US industry must begin to develop and use new technologies, new design strategies, and new industrial processes. However, the current fragmentation of the industry makes research by individual companies prohibitively expensive, and retards innovation. This research program addresses the need to increase the energy efficiency of industrialized housing. Two research centers have responsibility for the program: the Center for Housing Innovation at the University of Oregon and the Florida Solar Energy Center, a research institute of the University of Central Florida. The two organizations provide complementary architectural, systems engineering, and industrial engineering capabilities. In 1989 we worked on these tasks: the formation of a steering committee; the development of a multiyear research plan; analysis of the US industrialized housing industry; assessment of foreign technology; assessment of industrial applications; analysis of computerized design and evaluation tools; and assessment of energy performance of baseline and advanced industrialized housing concepts. Our goal is to develop techniques to produce marketable industrialized housing that is 25% more energy efficient that the most stringent US residential codes now require, and that costs less. Energy efficiency is the focus of the research, but it is viewed in the context of production and design. 63 refs.

  10. Industrial energy-efficiency-improvement program

    SciTech Connect

    Not Available

    1980-12-01

    Progress made by industry toward attaining the voluntary 1980 energy efficiency improvement targets is reported. The mandatory reporting population has been expanded from ten original industries to include ten additional non-targeted industries and all corporations using over one trillion Btu's annually in any manufacturing industry. The ten most energy intensive industries have been involved in the reporting program since the signing of the Energy Policy and Conservation Act and as industrial energy efficiency improvement overview, based primarily on information from these industries (chemicals and allied products; primary metal industry; petroleum and coal products; stone, clay, and glass products; paper and allied products; food and kindred products; fabricated metal products; transportation equipment; machinery, except electrical; and textile mill products), is presented. Reports from industries, now required to report, are included for rubber and miscellaneous plastics; electrical and electronic equipment; lumber and wood; and tobacco products. Additional data from voluntary submissions are included for American Gas Association; American Hotel and Motel Association; General Telephone and Electronics Corporation; and American Telephone and Telegraph Company. (MCW)

  11. Productivity benefits of industrial energy efficiency measures

    SciTech Connect

    Worrell, Ernst; Laitner, John A.; Michael, Ruth; Finman, Hodayah

    2004-08-30

    We review the relationship between energy efficiency improvement measures and productivity in industry. We review over 70 industrial case studies from widely available published databases, followed by an analysis of the representation of productivity benefits in energy modeling. We propose a method to include productivity benefits in the economic assessment of the potential for energy efficiency improvement. The case-study review suggests that energy efficiency investments can provide a significant boost to overall productivity within industry. If this relationship holds, the description of energy-efficient technologies as opportunities for larger productivity improvements has significant implications for conventional economic assessments. The paper explores the implications this change in perspective on the evaluation of energy-efficient technologies for a study of the iron and steel industry in the US. This examination shows that including productivity benefits explicitly in the modeling parameters would double the cost-effective potential for energy efficiency improvement, compared to an analysis excluding those benefits. We provide suggestions for future research in this important area.

  12. Productivity benefits of industrial energy efficiency measures.

    SciTech Connect

    Worrell, Ernst; Laitner, John A.; Michael, Ruth; Finman, Hodayah

    2004-08-30

    We review the relationship between energy efficiency improvement measures and productivity in industry. We review over 70 industrial case studies from widely available published databases, followed by an analysis of the representation of productivity benefits in energy modeling. We propose a method to include productivity benefits in the economic assessment of the potential for energy efficiency improvement. The case-study review suggests that energy efficiency investments can provide a significant boost to overall productivity within industry. If this relationship holds, the description of energy-efficient technologies as opportunities for larger productivity improvements has significant implications for conventional economic assessments. The paper explores the implications this change in perspective on the evaluation of energy-efficient technologies for a study of the iron and steel industry in the US. This examination shows that including productivity benefits explicitly in the mode ling parameters would double the cost-effective potential for energy efficiency improvement, compared to an analysis excluding those benefits. We provide suggestions for future research in this important area.

  13. GEF small grants programme - overview

    SciTech Connect

    1997-12-01

    This paper describes the GEF small grants program which seeks to enhance the role of households and communities in conserving global biodiversity, mitigating global climate change, and protecting international waters. Grants up to $50k have been granted for projects in 33 countries, with plans for 12 other countries. The author describes the framework that the program works under, and the methodology followed in developing and planning projects. The approach to climate change concerns is to emphasize the development of non-carbon energy development activities to provide energy sources and economic development.

  14. Energy efficient industrialized housing research program

    SciTech Connect

    Berg, R.; Brown, G.Z.; Finrow, J.; Kellett, R.; McDonald, M.; McGinn, B.; Ryan, P.; Sekiguchi, Tomoko . Center for Housing Innovation); Chandra, S.; Elshennawy, A.K.; Fairey, P.; Harrison, J.; Maxwell, L.; Roland, J.; Swart, W. )

    1990-02-01

    This report summarizes three documents: Multiyear Research Plan, Volume I FY 1989 Task Reports, and Volume II Appendices. These documents describe tasks that were undertaken from November 1988 to December 1989, the first year of the project. Those tasks were: (1) the formation of a steering committee, (2) the development of a multiyear research plan, (3) analysis of the US industrialized housing industry, (4) assessment of foreign technology, (5) assessment of industrial applications, (6) analysis of computerized design and evaluation tools, and (7) assessment of energy performance of baseline and advanced industrialized housing concepts. While this document summarizes information developed in each task area, it doesn't review task by task, as Volume I FY 1989 Task Reports does, but rather treats the subject of energy efficient industrialized housing as a whole to give the reader a more coherent view. 7 figs., 9 refs.

  15. Industrial Compressed Air System Energy Efficiency Guidebook.

    SciTech Connect

    United States. Bonneville Power Administration.

    1993-12-01

    Energy efficient design, operation and maintenance of compressed air systems in industrial plants can provide substantial reductions in electric power and other operational costs. This guidebook will help identify cost effective, energy efficiency opportunities in compressed air system design, re-design, operation and maintenance. The guidebook provides: (1) a broad overview of industrial compressed air systems, (2) methods for estimating compressed air consumption and projected air savings, (3) a description of applicable, generic energy conservation measures, and, (4) a review of some compressed air system demonstration projects that have taken place over the last two years. The primary audience for this guidebook includes plant maintenance supervisors, plant engineers, plant managers and others interested in energy management of industrial compressed air systems.

  16. Emerging energy-efficient industrial technologies

    SciTech Connect

    Martin, N.; Worrell, E.; Ruth, M.; Price, L.; Elliott, R.N.; Shipley, A.M.; Thorne, J.

    2000-10-01

    U.S. industry consumes approximately 37 percent of the nation's energy to produce 24 percent of the nation's GDP. Increasingly, industry is confronted with the challenge of moving toward a cleaner, more sustainable path of production and consumption, while increasing global competitiveness. Technology will be essential for meeting these challenges. At some point, businesses are faced with investment in new capital stock. At this decision point, new and emerging technologies compete for capital investment alongside more established or mature technologies. Understanding the dynamics of the decision-making process is important to perceive what drives technology change and the overall effect on industrial energy use. The assessment of emerging energy-efficient industrial technologies can be useful for: (1) identifying R&D projects; (2) identifying potential technologies for market transformation activities; (3) providing common information on technologies to a broad audience of policy-makers; and (4) offering new insights into technology development and energy efficiency potentials. With the support of PG&E Co., NYSERDA, DOE, EPA, NEEA, and the Iowa Energy Center, staff from LBNL and ACEEE produced this assessment of emerging energy-efficient industrial technologies. The goal was to collect information on a broad array of potentially significant emerging energy-efficient industrial technologies and carefully characterize a sub-group of approximately 50 key technologies. Our use of the term ''emerging'' denotes technologies that are both pre-commercial but near commercialization, and technologies that have already entered the market but have less than 5 percent of current market share. We also have chosen technologies that are energy-efficient (i.e., use less energy than existing technologies and practices to produce the same product), and may have additional ''non-energy benefits.'' These benefits are as important (if not more important in many cases) in influencing

  17. Energy Efficient Industrialized Housing Research Program

    SciTech Connect

    Not Available

    1992-03-01

    Six area reported progress in the Energy Efficient Industrialized Housing Research Program during FY 1991. As part of Industry Guidance, meetings were held with steering and technical committees in computers, housing design and manufacturing. This task area enables the program to benefit from the expertise of industry representatives and communicate research results directly to them. As part of the Design Process performance specifications were being developed for the future housing system designed last year. These house designs coordinate and optimize predicted and desirable advances in computerized design processes, materials, components, and manufacturing automation to achieve energy efficiency at reduced first cost. Energy design software were being developed for CAD systems, stressed skin insulating core panel manufacturers; and a prototype energy sales tool. A prototype design was to be developed to integrate one or more subsystems with the building skin. As part of the Manufacturing Process we are developing a manufacturing process simulation and data base to help current and new entrants to the industrialized housing industry in assessing the impact of implementing new manufacturing techniques. For Evaluation we are developing testing plans for six units of housing on the UO campus and the stressed skin insulating core house to be constructed in Oregon. The DOW Chemical test structure will be retrofitted with a tile roof and retested to compare to the dome and conventional construction structures. Calibration of the wind tunnel will be completed so that laboratory tests can be conducted to simulate the ventilation cooling efficiency of houses in design. Research utilization and program management were either aspects of this program.

  18. Industrial energy efficiency opportunities in Ukraine

    SciTech Connect

    Somasundaram, S.; Parker, S.; Evans, M.; Brown, D.

    1999-07-01

    As part of the energy assistance program to help Ukraine shut down the Chornobyl nuclear reactors, The US Department of Energy (DOE) asked the Pacific Northwest National Laboratory (PNNL) to identify and appraise industrial energy efficiency projects in Ukraine. The industrial sector currently accounts for over 60% of Ukraine's energy consumption. Most industrial enterprises in Ukraine use energy very inefficiently because the former Communist system provided few incentives to conserve energy or even account for its cost. Since 1994, however, the country's energy prices have risen close to world levels, and Ukraine finds itself saddled with very high energy costs. The Ukrainian Government is also under pressure to lower natural gas imports and reduce the country's trade imbalance with Russia. As a result, incentives to save energy in Ukraine are now great, and the market for energy efficiency products is growing. The Ukrainian Government estimates that this market will generate from $700 million to $1 billion worth of new product sales by the year 2000. However, few industrial enterprises have the money necessary for large-scale energy efficiency improvements. Therefore, one of the main goals of this project is to help the most promising enterprises obtain financing for energy efficiency projects form a variety of financial institutions, or through new, creative financing mechanisms. The project has involved several site visits to different industrial plants in Ukraine to gather preliminary data on the facilities. Most of these plants are in the process of being privatized or have been recently privatized and are financially sound. Among the plants visited have been a glass manufacturing plant, a coke-chemical plant, a paper mill, an alumina plant, a tire factory, a food processing plant, an iron mine, a metallurgical firm, and a steel cable factory. Following a preliminary analysis of the site-specific data obtained during the first visit, a limited number of

  19. Improving GEFS Weather Forecasts for Indian Monsoon with Statistical Downscaling

    NASA Astrophysics Data System (ADS)

    Agrawal, Ankita; Salvi, Kaustubh; Ghosh, Subimal

    2014-05-01

    Weather forecast has always been a challenging research problem, yet of a paramount importance as it serves the role of 'key input' in formulating modus operandi for immediate future. Short range rainfall forecasts influence a wide range of entities, right from agricultural industry to a common man. Accurate forecasts actually help in minimizing the possible damage by implementing pre-decided plan of action and hence it is necessary to gauge the quality of forecasts which might vary with the complexity of weather state and regional parameters. Indian Summer Monsoon Rainfall (ISMR) is one such perfect arena to check the quality of weather forecast not only because of the level of intricacy in spatial and temporal patterns associated with it, but also the amount of damage it can cause (because of poor forecasts) to the Indian economy by affecting agriculture Industry. The present study is undertaken with the rationales of assessing, the ability of Global Ensemble Forecast System (GEFS) in predicting ISMR over central India and the skill of statistical downscaling technique in adding value to the predictions by taking them closer to evidentiary target dataset. GEFS is a global numerical weather prediction system providing the forecast results of different climate variables at a fine resolution (0.5 degree and 1 degree). GEFS shows good skills in predicting different climatic variables but fails miserably over rainfall predictions for Indian summer monsoon rainfall, which is evident from a very low to negative correlation values between predicted and observed rainfall. Towards the fulfilment of second rationale, the statistical relationship is established between the reasonably well predicted climate variables (GEFS) and observed rainfall. The GEFS predictors are treated with multicollinearity and dimensionality reduction techniques, such as principal component analysis (PCA) and least absolute shrinkage and selection operator (LASSO). Statistical relationship is

  20. Rho GEFs and GAPs: Emerging integrators of extracellular matrix signaling

    PubMed Central

    Kutys, Matthew L; Yamada, Kenneth M

    2015-01-01

    Investigating cell migration in 3D settings has revealed that specific extracellular matrix environments require differential activities of the Rho GTPases for efficient migration. However, it is largely unknown how the activities of specific Rho GTPases are modulated to direct cell migration in response to different extracellular matrix cues. We have recently reported that extracellular matrix-dependent regulation of a specific Rho GEF is a fundamental mechanism governing cell migration in different microenvironments, providing a direct mechanism for extracellular matrix-specific regulation of Rho GTPase activity directing cell motility. We discovered that the Rho GEF βPix has a unique function during cell migration in fibrillar collagen environments by restraining RhoA signaling through a conserved signaling axis involving Cdc42 and the Rho GAP srGAP1. In this Commentary, we expand upon this new pathway and discuss potential mechanotransductive and therapeutic applications. Additionally, we speculate on a generalized role for Rho GEFs and GAPs in providing localized, context-dependent responses to the cellular microenvironment during cell migration and other cellular processes. PMID:25862162

  1. Energy efficiency in buildings, industry and transportation

    NASA Astrophysics Data System (ADS)

    Milovanovic, Dobrica; Babic, Milun; Jovicic, Nebojsa; Gordic, Dusan

    2012-11-01

    This paper reviews the literature concerning the energy saving and outlines the importance of energy efficiency, particularly in three the most important areas: buildings, industry and transportation. Improving energy efficiency plays a crucial role in minimizing the societal and environmental impacts of economic growth and offers a powerful tool for achieving sustainable development by reducing the need for investment in new infrastructure, by cutting fuel costs, and by increasing competitiveness for businesses and welfare for consumers. It creates environmental benefits through reduced emissions of greenhouse gases and local air pollutants. It can offer social benefits in the form of increased energy security (through reduced dependence on fossil fuels, particularly when imported) and better energy services.

  2. Energy efficiency in buildings, industry and transportation

    NASA Astrophysics Data System (ADS)

    Milovanovic, Dobrica; Babic, Milun; Jovicic, Nebojsa; Gordic, Dusan

    2013-01-01

    This paper reviews the literature concerning the energy saving and outlines the importance of energy efficiency, particularly in three the most important areas: buildings, industry and transportation. Improving energy efficiency plays a crucial role in minimizing the societal and environmental impacts of economic growth and offers a powerful tool for achieving sustainable development by reducing the need for investment in new infrastructure, by cutting fuel costs, and by increasing competitiveness for businesses and welfare for consumers. It creates environmental benefits through reduced emissions of greenhouse gases and local air pollutants. It can offer social benefits in the form of increased energy security (through reduced dependence on fossil fuels, particularly when imported) and better energy services.

  3. Setting the Standard for Industrial Energy Efficiency

    SciTech Connect

    McKane, Aimee; Williams, Robert; Perry, Wayne; Li, Tienan

    2007-06-01

    Industrial motor-driven systems use more than 2194 billionkWh annually on a global basis and offer one of the largest opportunitiesfor energy savings.1 The International Energy Agency estimates thatoptimization of motor driven systems could reduce global electricitydemand by 7 percent through the application of commercially availabletechnologies and using well-tested engineering practices. Yet manyindustrial firms remain either unaware of or unable to achieve theseenergy savings. The same factors that make it so challenging to achieveand sustain energy efficiency in motor-driven systems (complexity,frequent changes) apply to the production processes that they support.Yet production processes typically operate within a narrow band ofacceptable performance. These processes are frequently incorporated intoISO 9000/14000 quality and environmental management systems, whichrequire regular, independent audits to maintain ISO certification, anattractive value for international trade. It is our contention that acritical step in achieving and sustaining energy efficiency ofmotor-driven systems specifically, and industrial energy efficiencygenerally, is the adoption of a corporate energy management standard thatis consistent with current industrial quality and environmentalmanagement systems such as ISO. Several energy management standardscurrently exist (US, Denmark, Ireland, Sweden) and specifications(Germany, Netherlands) others are planned (China, Spain, Brazil, Korea).This paper presents the current status of energy management standardsdevelopment internationally, including an analysis of their sharedfeatures and differences, in terms of content, promulgation, andimplementation. The purpose of the analysis is to describe the currentstate of "best practices" for this emerging area of energy efficiencypolicymaking and tosuggest next steps toward the creation of a trulyinternational energy management standard that is consistent with the ISOprinciples of measurement

  4. Barriers to Industrial Energy Efficiency - Study (Appendix A), June 2015

    SciTech Connect

    2015-06-01

    This study examines barriers that impede the adoption of energy efficient technologies and practices in the industrial sector, and identifies successful examples and opportunities to overcome these barriers. Three groups of energy efficiency technologies and measures were examined: industrial end-use energy efficiency, industrial demand response, and industrial combined heat and power. This study also includes the estimated economic benefits from hypothetical Federal energy efficiency matching grants, as directed by the Act.

  5. Barriers to Industrial Energy Efficiency - Report to Congress, June 2015

    SciTech Connect

    2015-06-01

    This report examines barriers that impede the adoption of energy efficient technologies and practices in the industrial sector, and identifies successful examples and opportunities to overcome these barriers. Three groups of energy efficiency technologies and measures were examined: industrial end-use energy efficiency, industrial demand response, and industrial combined heat and power. This report also includes the estimated economic benefits from hypothetical Federal energy efficiency matching grants, as directed by the Act.

  6. 77 FR 20615 - DAU Industry Day: “Affordability, Efficiency, and the Industrial Base”

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-05

    ... of the Secretary DAU Industry Day: ``Affordability, Efficiency, and the Industrial Base'' AGENCY..., efficiency, and the industrial base. After a variety of presenters, the session will conclude with Mr. Frank... maintaining a healthy industrial base. Following the plenary session, each company will have the...

  7. [Eco-efficiency of industry sectors for China].

    PubMed

    Mao, Jian-Su; Zeng, Run; Du, Yan-Chun; Jiang, Pan

    2010-11-01

    Industry is the main source of resource consumption and environmental emissions of China, and the analyzing of eco-efficiencies for industrial sectors may provide essential information for the restructure of industrial system and the improvement of environmental quality of China. The industry composition was analyzed and the relationship between the industry and its environment was established. The energy consumption and the main environmental emissions such as wastewater, solid waste, industry SO2, smog, dust were chosen as the environmental impact categories, the corresponding eco-efficiencies of industry were defined and estimated for China in 2007. The results are as follows: the energy efficiency is 615.5 x 10(4) yuan x ktce(-1), the wastewater and solid waste related eco-efficiencies are 54.3 x 10(4) yuan x kt(-1) and 1.9 x 10(4) yuan x t(-1), respectively; industry SO2, smog, dust related eco-efficiencies are 59.6 x 10(4), 169.2 x 10(4) and 184.6 x 10(4) yuan x t(-1), respectively. The eco-efficiencies of 39 industry sectors were analyzed extensively and the results showed that, the distribution of industrial sectors in above eco-efficiencies is uneven, and the order of industrial sector in industry will vary with industry sector and eco-efficiency category. Meanwhile, for the same category of eco-efficiency, the values for different industry sectors may change from centuplicate to several thousand folds, therefore a great possibility for the improvement of industry eco-efficiency is exist in the restructure of industry system. PMID:21250467

  8. 78 FR 11996 - Energy Efficiency Program for Commercial and Industrial Equipment: Commercial and Industrial Pumps

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-21

    ... efficiency of commercial and industrial pumps. (76 FR 34192, June 13, 2011). DOE subsequently published a...; ] DEPARTMENT OF ENERGY 10 CFR Part 431 RIN 1904-AC54 Energy Efficiency Program for Commercial and Industrial Equipment: Commercial and Industrial Pumps AGENCY: Office of Energy Efficiency and Renewable...

  9. I'm coming to GEF you: Regulation of RhoGEFs during cell migration.

    PubMed

    Goicoechea, Silvia M; Awadia, Sahezeel; Garcia-Mata, Rafael

    2014-01-01

    Cell migration is a highly regulated multistep process that requires the coordinated regulation of cell adhesion, protrusion, and contraction. These processes require numerous protein-protein interactions and the activation of specific signaling pathways. The Rho family of GTPases plays a key role in virtually every aspect of the cell migration cycle. The activation of Rho GTPases is mediated by a large and diverse family of proteins; the guanine nucleotide exchange factors (RhoGEFs). GEFs work immediately upstream of Rho proteins to provide a direct link between Rho activation and cell-surface receptors for various cytokines, growth factors, adhesion molecules, and G protein-coupled receptors. The regulated targeting and activation of RhoGEFs is essential to coordinate the migratory process. In this review, we summarize the recent advances in our understanding of the role of RhoGEFs in the regulation of cell migration. PMID:25482524

  10. Energy efficient industrialized housing research program

    SciTech Connect

    Berg, R.; Brown, G.Z.; Finrow, J.; Kellett, R.; McDonald, M.; McGinn, B.; Ryan, P.; Sekiguchi, Tomoko . Center for Housing Innovation); Chandra, S.; Elshennawy, A.K.; Fairey, P.; Harrison, J.; Mazwell, L.; Roland, J.; Swart, W. )

    1989-12-01

    This document describes the research work completed in five areas in fiscal year 1989. (1) The analysis of the US industrialized housing industry includes statistics, definitions, a case study, and a code analysis. (2) The assessment of foreign technology reviews the current status of design, manufacturing, marketing, and installation of industrialized housing primarily in Sweden and Japan. (3) Assessment of industrialization applications reviews housing production by climate zone, has a cost and energy comparison of Swedish and US housing, and discusses future manufacturing processes and emerging components. (4) The state of computer use in the industry is described and a prototype design tool is discussed. (5) Side by side testing of industrialized housing systems is discussed.

  11. Maximizing industrial infrastructure efficiency in Iceland

    NASA Astrophysics Data System (ADS)

    Ingason, Helgi Thor; Sigfusson, Thorsteinn I.

    2010-08-01

    As a consequence of the increasing aluminum production in Iceland, local processing of aluminum skimmings has become a feasible business opportunity. A recycling plant for this purpose was built in Helguvik on the Reykjanes peninsula in 2003. The case of the recycling plant reflects increased concern regarding environmental aspects of the industry. An interesting characteristic of this plant is the fact that it is run in the same facilities as a large fishmeal production installation. It is operated by the same personnel and uses—partly—the same equipment and infrastructure. This paper reviews the grounds for these decisions and the experience of this merger of a traditional fish melting industry and a more recent aluminum melting industry after 6 years of operation. The paper is written by the original entrepreneurs behind the company, who provide observations on how the aluminum industry in Iceland has evolved since the starting of Alur’s operation and what might be expected in the near future.

  12. Refractories for Industrial Processing. Opportunities for Improved Energy Efficiency

    SciTech Connect

    Hemrick, James G.; Hayden, H. Wayne; Angelini, Peter; Moore, Robert E.; Headrick, William L.

    2005-01-01

    Refractories are a class of materials of critical importance to manufacturing industries with high-temperature unit processes. This study describes industrial refractory applications and identifies refractory performance barriers to energy efficiency for processing. The report provides recommendations for R&D pathways leading to improved refractories for energy-efficient manufacturing and processing.

  13. 77 FR 54777 - Accelerating Investment in Industrial Energy Efficiency

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-05

    ... ] Executive Order 13624 of August 30, 2012 Accelerating Investment in Industrial Energy Efficiency By the... order to promote American manufacturing by helping to facilitate investments in energy efficiency at... authorities to overcome these barriers, and our efforts to support investment in industrial energy...

  14. Final Scientific Report - Wireless and Sensing Solutions Advancing Industrial Efficiency

    SciTech Connect

    Budampati, Rama; McBrady, Adam; Nusseibeh, Fouad

    2009-09-28

    The project team's goal for the Wireless and Sensing Solution Advancing Industrial Efficiency award (DE-FC36-04GO14002) was to develop, demonstrate, and test a number of leading edge technologies that could enable the emergence of wireless sensor and sampling systems for the industrial market space. This effort combined initiatives in advanced sensor development, configurable sampling and deployment platforms, and robust wireless communications to address critical obstacles in enabling enhanced industrial efficiency.

  15. Energy efficiency opportunities in the brewery industry

    SciTech Connect

    Worrell, Ernst; Galitsky, Christina; Martin, Nathan

    2002-06-28

    Breweries in the United States spend annually over $200 Million on energy. Energy consumption is equal to 3-8% of the production costs of beer, making energy efficiency improvement an important way to reduce costs, especially in times of high energy price volatility. After a summary of the beer making process and energy use, we examine energy efficiency opportunities available for breweries. We provide specific primary energy savings for each energy efficiency measure based on case studies that have implemented the measures, as well as references to technical literature. If available, we have also listed typical payback periods. Our findings suggest that there may still be opportunities to reduce energy consumption cost-effectively for breweries. Major brewing companies have and will continue to spend capital on cost effective measures that do not impact the quality of the beer. Further research on the economics of the measures, as well as their applicability to different brewing practices, is needed to assess implementation of selected technologies at individual breweries.

  16. India's Fertilizer Industry: Productivity and Energy Efficiency

    SciTech Connect

    Schumacher, K.; Sathaye, J.

    1999-07-01

    Historical estimates of productivity growth in India's fertilizer sector vary from indicating an improvement to a decline in the sector's productivity. The variance may be traced to the time period of study, source of data for analysis, and type of indices and econometric specifications used for reporting productivity growth. Our analysis shows that in the twenty year period, 1973 to 1993, productivity in the fertilizer sector increased by 2.3% per annum. An econometric analysis reveals that technical progress in India's fertilizer sector has been biased towards the use of energy, while it has been capital and labor saving. The increase in productivity took place during the era of total control when a retention price system and distribution control was in effect. With liberalization of the fertilizer sector and reduction of subsidies productivity declined substantially since the early 1990s. Industrial policies and fiscal incentives still play a major role in the Indian fertilizer sect or. As substantial energy savings and carbon reduction potential exists, energy policies can help overcome barriers to the adoption of these measures in giving proper incentives and correcting distorted prices.

  17. Clean Energy Manufacturing Initiative Industrial Efficiency and Energy Productivity

    ScienceCinema

    Selldorff, John; Atwell, Monte

    2014-12-03

    Industrial efficiency and low-cost energy resources are key components to increasing U.S. energy productivity and makes the U.S. manufacturing sector more competitive. Companies find a competitive advantage in implementing efficiency technologies and practices, and technologies developed and manufactured in the U.S. enable greater competitiveness economy-wide.

  18. Clean Energy Manufacturing Initiative Industrial Efficiency and Energy Productivity

    SciTech Connect

    Selldorff, John; Atwell, Monte

    2014-09-23

    Industrial efficiency and low-cost energy resources are key components to increasing U.S. energy productivity and makes the U.S. manufacturing sector more competitive. Companies find a competitive advantage in implementing efficiency technologies and practices, and technologies developed and manufactured in the U.S. enable greater competitiveness economy-wide.

  19. Neonatal epilepsy syndromes and GEFS+: mechanistic considerations.

    PubMed

    Burgess, Daniel L

    2005-01-01

    Genetic analyses of familial epilepsies over the past decade have identified mutations in several different ion channel genes that result in neonatal or early-onset seizure disorders, including benign familial neonatal convulsions (BFNC), generalized epilepsy with febrile seizures plus (GEFS+), and severe myoclonic epilepsy of infancy (SMEI). These genes encode voltage-gated Na+ channel subunits (SCN1A, SCN2A, SCN1B), voltage-gated K+ channel subunits (KCNQ2, KCNQ3), and a ligand-gated neurotransmitter receptor subunit (GABRG2). While the opportunity to genotype patients for mutations in these genes can have an immediate and significant impact on our ability to diagnose and provide genetic counseling to patients, the ultimate goal is to use this molecular knowledge to develop effective treatments and cures for each disorder. This will necessitate elucidation of the molecular, cellular, and network mechanisms that translate ion channel defects into specific epilepsy phenotypes. The functional analysis of epileptogenic channel mutations in vitro and in vivo has already provided a vast amount of raw biophysical data, but attempts to interpret these data to explain clinical phenotypes so far appear to raise as many questions as they answer. Nevertheless, patterns are beginning to emerge from these early studies that will help define the full scope of the challenges ahead while simultaneously providing the foundation of future efforts to overcome them. Here, I discuss some of the potential mechanisms that have been uncovered recently linking mutant ion channel genes to neonatal epilepsy syndromes and GEFS+. PMID:16359473

  20. Energy-Efficiency Improvement Opportunities for the Textile Industry

    SciTech Connect

    China Energy Group; Hasanbeigi, Ali

    2010-09-29

    The textile industry is one of the most complicated manufacturing industries because it is a fragmented and heterogeneous sector dominated by small and medium enterprises (SMEs). Energy is one of the main cost factors in the textile industry. Especially in times of high energy price volatility, improving energy efficiency should be a primary concern for textile plants. There are various energy-efficiency opportunities that exist in every textile plant, many of which are cost-effective. However, even cost-effective options often are not implemented in textile plants mostly because of limited information on how to implement energy-efficiency measures, especially given the fact that a majority of textile plants are categorized as SMEs and hence they have limited resources to acquire this information. Know-how on energy-efficiency technologies and practices should, therefore, be prepared and disseminated to textile plants. This guidebook provides information on energy-efficiency technologies and measures applicable to the textile industry. The guidebook includes case studies from textile plants around the world and includes energy savings and cost information when available. First, the guidebook gives a brief overview of the textile industry around the world, with an explanation of major textile processes. An analysis of the type and the share of energy used in different textile processes is also included in the guidebook. Subsequently, energy-efficiency improvement opportunities available within some of the major textile sub-sectors are given with a brief explanation of each measure. The conclusion includes a short section dedicated to highlighting a few emerging technologies in the textile industry as well as the potential for the use of renewable energy in the textile industry.

  1. Industrial Energy Efficiency: Designing Effective State Programs for the Industrial Sector

    SciTech Connect

    Amelie Goldberg; Taylor, Robert P.; Hedman, Bruce

    2014-03-21

    This report provides state regulators, utilities, and other program administrators with an overview of U.S. industrial energy efficiency programs and assesses some of the key features of programs that have generated increased energy savings.

  2. Industrial Technologies Program - A Clean, Secure Energy Future via Industrial Energy Efficiency

    SciTech Connect

    2010-05-01

    The Industrial Technologies Program (ITP) leads the national effort to save energy and reduce greenhouse gas emissions in the largest energy-using sector of the U.S. economy. ITP drives energy efficiency improvements and carbon dioxide reductions throughout the manufacturing supply chain, helping develop and deploy innovative technologies that transform the way industry uses energy.

  3. The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis

    PubMed Central

    Wang, Ning; Wang, Mo; Zhu, Yi-Hua; Grosel, Timothy W.; Sun, Daokun; Kudryashov, Dmitri S.; Wu, Jian-Qiu

    2015-01-01

    Rho GTPases, activated by Rho guanine nucleotide exchange factors (GEFs), are conserved molecular switches for signal transductions that regulate diverse cellular processes, including cell polarization and cytokinesis. The fission yeast Schizosaccharomyces pombe has six Rho GTPases (Cdc42 and Rho1–Rho5) and seven Rho GEFs (Scd1, Rgf1–Rgf3, and Gef1–Gef3). The GEFs for Rho2–Rho5 have not been unequivocally assigned. In particular, Gef3, the smallest Rho GEF, was barely studied. Here we show that Gef3 colocalizes with septins at the cell equator. Gef3 physically interacts with septins and anillin Mid2 and depends on them to localize. Gef3 coprecipitates with GDP-bound Rho4 in vitro and accelerates nucleotide exchange of Rho4, suggesting that Gef3 is a GEF for Rho4. Consistently, Gef3 and Rho4 are in the same genetic pathways to regulate septum formation and/or cell separation. In gef3∆ cells, the localizations of two potential Rho4 effectors—glucanases Eng1 and Agn1—are abnormal, and active Rho4 level is reduced, indicating that Gef3 is involved in Rho4 activation in vivo. Moreover, overexpression of active Rho4 or Eng1 rescues the septation defects of mutants containing gef3∆. Together our data support that Gef3 interacts with the septin complex and activates Rho4 GTPase as a Rho GEF for septation in fission yeast. PMID:25411334

  4. New trends in industrial energy efficiency in the Mexico iron and steel industry

    SciTech Connect

    Ozawa, Leticia; Martin, Nathan; Worrell, Ernst; Price, Lynn; Sheinbaum, Claudia

    1999-07-31

    Energy use in the Mexican industrial sector experienced important changes in the last decade related to changes in the Mexican economy. In previous studies, we have shown that a real change in energy-intensity was the most important factor in the overall decline of energy use and CO2 emissions in the Mexican industrial sector. Real changes in energy intensity were explained by different factors, depending on the industrial sub-sector. In this paper, we analyze the factors that influenced energy use in the Mexican iron and steel industry, the largest energy consuming and energy-intensive industry in the country. To understand the trends in this industry we used a decomposition analysis based on physical indicators to decompose the changes in intra-sectoral structural changes and efficiency improvements. Also, we use a structure-efficiency analysis for international comparisons, considering industrial structure and the best available technology. In 1995, Mexican iron and steel industry consumed 17.7 percent of the industrial energy consumption. Between 1970 and 1995, the steel production has increased with an annual growth rate of 4.7 percent, while the specific energy consumption (SEC) has decreased from 28.4 to 23.8 GJ/tonne of crude steel. This reduction was due to energy efficiency improvements (disappearance of the open hearth production, increase of the share of the continuous casting) and to structural changes as well (increase of the share of scrap input in the steelmaking).

  5. Energy efficiency programs and policies in the industrial sector in industrialized countries

    SciTech Connect

    Galitsky, Christina; Price, Lynn; Worrell, Ernst

    2004-06-01

    About 37% of the primary energy consumed both in the U.S. and globally is used by the industrial sector. A variety of energy efficiency policies and programs have been implemented throughout the world in an effort to improve the energy efficiency of this sector. This report provides an overview of these policies and programs in twelve industrialized nations and the European Union (EU). We focus on energy efficiency products and services that are available to industrial consumers, such as reports, guidebooks, case studies, fact sheets, profiles, tools, demonstrations, roadmaps and benchmarking. We also focus on the mechanisms to communicate the availability and features of these products and services and to disseminate them to the industrial consumers who can use them. Communication channels include customer information centers and websites, conferences and trade shows, workshops and other training mechanisms, financial assistance programs, negotiated agreements, newsletters, publicity, assessments, tax and subsidy schemes and working groups. In total, over 30 types of industrial sector energy efficiency products, services and delivery channels have been identified in the countries studied. Overall, we found that the United States has a large variety of programs and offers industry a number of supporting programs for improving industrial energy efficiency. However, there are some products and services found in other industrialized countries that are not currently used in the U.S., including benchmarking programs, demonstration of commercialized technologies and provision of energy awareness promotion materials to companies. Delivery mechanisms found in other industrialized countries that are not employed in the U.S. include negotiated agreements, public disclosure and national-level tax abatement for energy-efficient technologies.

  6. Measuring industrial energy efficiency: Physical volume versus economic value

    SciTech Connect

    Freeman, S.L.; Niefer, M.J.; Roop, J.M.

    1996-12-01

    This report examines several different measures of industrial output for use in constructing estimates of industrial energy efficiency and discusses some reasons for differences between the measures. Estimates of volume-based measures of output, as well as 3 value-based measures of output (value of production, value of shipments, and value added), are evaluated for 15 separate 4-digit industries. Volatility, simple growth rate, and trend growth rate estimates are made for each industry and each measure of output. Correlations are made between the volume- and value-based measures of output. Historical energy use data are collected for 5 of the industries for making energy- intensity estimates. Growth rates in energy use, energy intensity, and correlations between volume- and value-based measures of energy intensity are computed. There is large variability in growth trend estimates both long term and from year to year. While there is a high correlation between volume- and value-based measures of output for a few industries, typically the correlation is low, and this is exacerbated for estimates of energy intensity. Analysis revealed reasons for these low correlations. It appears that substantial work must be done before reliable measures of trends in the energy efficiency of industry can be accurately characterized.

  7. Air Force Achieves Fuel Efficiency through Industry Best Practices

    SciTech Connect

    2012-12-01

    The U.S. Air Force’s Air Mobility Command (AMC) is changing the way it does business. It is saving energy and money through an aircraft fleet fuel-efficiency program inspired by private industry best practices and ideas resulting from the empowered fuel savings culture.

  8. Energy Matters: An invitation to Chat About Industrial Efficiency

    SciTech Connect

    Hogan, Kathleen

    2011-01-01

    Do you have questions or ideas about how the U.S. Department of Energy can contribute to global competitiveness through industrial efficiency? Dr. Kathleen Hogan would like to hear them. Submit your questions via: Email ( newmedia@hq.doe.gov ) Twitter ( @Energy ) Facebook ( Facebook.com/Energygov ) **LIVE CHAT IS EXPIRED**

  9. Energy Matters: An invitation to Chat About Industrial Efficiency

    ScienceCinema

    Hogan, Kathleen

    2013-05-29

    Do you have questions or ideas about how the U.S. Department of Energy can contribute to global competitiveness through industrial efficiency? Dr. Kathleen Hogan would like to hear them. Submit your questions via: Email ( newmedia@hq.doe.gov ) Twitter ( @Energy ) Facebook ( Facebook.com/Energygov ) **LIVE CHAT IS EXPIRED**

  10. Energy Efficiency Improvement in the Petroleum RefiningIndustry

    SciTech Connect

    Worrell, Ernst; Galitsky, Christina

    2005-05-01

    Information has proven to be an important barrier inindustrial energy efficiency improvement. Voluntary government programsaim to assist industry to improve energy efficiency by supplyinginformation on opportunities. ENERGY STAR(R) supports the development ofstrong strategic corporate energy management programs, by providingenergy management information tools and strategies. This paper summarizesENERGY STAR research conducted to develop an Energy Guide for thePetroleum Refining industry. Petroleum refining in the United States isthe largest in the world, providing inputs to virtually every economicsector, including the transport sector and the chemical industry.Refineries spend typically 50 percent of the cash operating costs (e.g.,excluding capital costs and depreciation) on energy, making energy amajor cost factor and also an important opportunity for cost reduction.The petroleum refining industry consumes about 3.1 Quads of primaryenergy, making it the single largest industrial energy user in the UnitedStates. Typically, refineries can economically improve energy efficiencyby 20 percent. The findings suggest that given available resources andtechnology, there are substantial opportunities to reduce energyconsumption cost-effectively in the petroleum refining industry whilemaintaining the quality of the products manufactured.

  11. On eco-efficient technologies to minimize industrial water consumption

    NASA Astrophysics Data System (ADS)

    Amiri, Mohammad C.; Mohammadifard, Hossein; Ghaffari, Ghasem

    2016-07-01

    Purpose - Water scarcity will further stress on available water systems and decrease the security of water in many areas. Therefore, innovative methods to minimize industrial water usage and waste production are of paramount importance in the process of extending fresh water resources and happen to be the main life support systems in many arid regions of the world. This paper demonstrates that there are good opportunities for many industries to save water and decrease waste water in softening process by substituting traditional with echo-friendly methods. The patented puffing method is an eco-efficient and viable technology for water saving and waste reduction in lime softening process. Design/methodology/approach - Lime softening process (LSP) is a very sensitive process to chemical reactions. In addition, optimal monitoring not only results in minimizing sludge that must be disposed of but also it reduces the operating costs of water conditioning. Weakness of the current (regular) control of LSP based on chemical analysis has been demonstrated experimentally and compared with the eco-efficient puffing method. Findings - This paper demonstrates that there is a good opportunity for many industries to save water and decrease waste water in softening process by substituting traditional method with puffing method, a patented eco-efficient technology. Originality/value - Details of the required innovative works to minimize industrial water usage and waste production are outlined in this paper. Employing the novel puffing method for monitoring of lime softening process results in saving a considerable amount of water while reducing chemical sludge.

  12. Design for energy efficiency: Energy efficient industrialized housing research program. Progress report

    SciTech Connect

    Kellett, R.; Berg, R.; Paz, A.; Brown, G.Z.

    1991-03-01

    Since 1989, the U.S. Department of Energy has sponsored the Energy Efficient Industrialized Housing research program (EEIH) to improve the energy efficiency of industrialized housing. Two research centers share responsibility for this program: The Center for Housing Innovation at the University of Oregon and the Florida Solar Energy Center, a research institute of the University of Central Florida. Additional funding is provided through the participation of private industry, state governments and utilities. The program is guided by a steering committee comprised of industry and government representatives. This report summarizes Fiscal Year (FY) 1990 activities and progress, and proposed activities for FY 1991 in Task 2.1 Design for Energy Efficiency. This task establishes a vision of energy conservation opportunities in critical regions, market segments, climate zones and manufacturing strategies significant to industrialized housing in the 21st Century. In early FY 1990, four problem statements were developed to define future housing demand scenarios inclusive of issues of energy efficiency, housing design and manufacturing. Literature surveys were completed to assess seven areas of influence for industrialized housing and energy conservation in the future. Fifty-five future trends were identified in computing and design process; manufacturing process; construction materials, components and systems; energy and environment; demographic context; economic context; and planning policy and regulatory context.

  13. Integrated Conformational and Lipid-Sensing Regulation of Endosomal ArfGEF BRAG2

    PubMed Central

    Aizel, Kaheina; Biou, Valérie; Navaza, Jorge; Duarte, Lionel V.; Campanacci, Valérie; Cherfils, Jacqueline; Zeghouf, Mahel

    2013-01-01

    The mechanisms whereby guanine nucleotide exchange factors (GEFs) coordinate their subcellular targeting to their activation of small GTPases remain poorly understood. Here we analyzed how membranes control the efficiency of human BRAG2, an ArfGEF involved in receptor endocytosis, Wnt signaling, and tumor invasion. The crystal structure of an Arf1–BRAG2 complex that mimics a membrane-bound intermediate revealed an atypical PH domain that is constitutively anchored to the catalytic Sec7 domain and interacts with Arf. Combined with the quantitative analysis of BRAG2 exchange activity reconstituted on membranes, we find that this PH domain potentiates nucleotide exchange by about 2,000-fold by cumulative conformational and membrane-targeting contributions. Furthermore, it restricts BRAG2 activity to negatively charged membranes without phosphoinositide specificity, using a positively charged surface peripheral to but excluding the canonical lipid-binding pocket. This suggests a model of BRAG2 regulation along the early endosomal pathway that expands the repertoire of GEF regulatory mechanisms. Notably, it departs from the auto-inhibitory and feedback loop paradigm emerging from studies of SOS and cytohesins. It also uncovers a novel mechanism of unspecific lipid-sensing by PH domains that may allow sustained binding to maturating membranes. PMID:24058294

  14. Energy efficiency opportunities in China. Industrial equipment and small cogeneration

    SciTech Connect

    1995-02-01

    A quick glance at comparative statistics on energy consumption per unit of industrial output reveals that China is one of the least energy efficient countries in the world. Energy waste not only impedes economic growth, but also creates pollution that threatens human health, regional ecosystems, and the global climate. China`s decision to pursue economic reform and encourage technology transfer from developed countries has created a window of opportunity for significant advances in energy efficiency. Policy changes, technical training, public education, and financing can help China realize its energy conservation potential.

  15. Technologies and Policies to Improve Energy Efficiency in Industry

    SciTech Connect

    Price, Lynn; Price, Lynn

    2008-03-01

    The industrial sector consumes nearly 40% of annual global primary energy use and is responsible for a similar share of global energy-related carbon dioxide (CO2) emissions. Many studies and actual experience indicate that there is considerable potential to reduce the amount of energy used to manufacture most commodities, concurrently reducing CO2 emissions. With the support of strong policies and programs, energy-efficient technologies and measures can be implemented that will reduce global CO2 emissions. A number of countries, including the Netherlands, the UK, and China, have experience implementing aggressive programs to improve energy efficiency and reduce related CO2 emissions from industry. Even so, there is no silver bullet and all options must be pursued if greenhouse gas emissions are to be constrained to the level required to avoid significant negative impacts from global climate change.

  16. Energy Efficient Industrialized Housing Research Program. Annual report, FY 1991

    SciTech Connect

    Not Available

    1992-03-01

    Six area reported progress in the Energy Efficient Industrialized Housing Research Program during FY 1991. As part of Industry Guidance, meetings were held with steering and technical committees in computers, housing design and manufacturing. This task area enables the program to benefit from the expertise of industry representatives and communicate research results directly to them. As part of the Design Process performance specifications were being developed for the future housing system designed last year. These house designs coordinate and optimize predicted and desirable advances in computerized design processes, materials, components, and manufacturing automation to achieve energy efficiency at reduced first cost. Energy design software were being developed for CAD systems, stressed skin insulating core panel manufacturers; and a prototype energy sales tool. A prototype design was to be developed to integrate one or more subsystems with the building skin. As part of the Manufacturing Process we are developing a manufacturing process simulation and data base to help current and new entrants to the industrialized housing industry in assessing the impact of implementing new manufacturing techniques. For Evaluation we are developing testing plans for six units of housing on the UO campus and the stressed skin insulating core house to be constructed in Oregon. The DOW Chemical test structure will be retrofitted with a tile roof and retested to compare to the dome and conventional construction structures. Calibration of the wind tunnel will be completed so that laboratory tests can be conducted to simulate the ventilation cooling efficiency of houses in design. Research utilization and program management were either aspects of this program.

  17. Technical Efficiency of Automotive Industry Cluster in Chennai

    NASA Astrophysics Data System (ADS)

    Bhaskaran, E.

    2012-07-01

    Chennai is also called as Detroit of India due to its automotive industry presence producing over 40 % of the India's vehicle and components. During 2001-2002, diagnostic study was conducted on the Automotive Component Industries (ACI) in Ambattur Industrial Estate, Chennai and in SWOT analysis it was found that it had faced problems on infrastructure, technology, procurement, production and marketing. In the year 2004-2005 under the cluster development approach (CDA), they formed Chennai auto cluster, under public private partnership concept, received grant from Government of India, Government of Tamil Nadu, Ambattur Municipality, bank loans and stake holders. This results development in infrastructure, technology, procurement, production and marketing interrelationships among ACI. The objective is to determine the correlation coefficient, regression equation, technical efficiency, peer weights, slack variables and return to scale of cluster before and after the CDA. The methodology adopted is collection of primary data from ACI and analyzing using data envelopment analysis (DEA) of input oriented Banker-Charnes-Cooper model. There is significant increase in correlation coefficient and the regression analysis reveals that for one percent increase in employment and net worth, the gross output increases significantly after the CDA. The DEA solver gives the technical efficiency of ACI by taking shift, employment, net worth as input data and quality, gross output and export ratio as output data. From the technical score and ranking of ACI, it is found that there is significant increase in technical efficiency of ACI when compared to CDA. The slack variables obtained clearly reveals the excess employment and net worth and no shortage of gross output. To conclude there is increase in technical efficiency of not only Chennai auto cluster in general but also Chennai auto components industries in particular.

  18. Power losses and the efficiency of industrially used gearboxes

    NASA Astrophysics Data System (ADS)

    Čarnogurská, Mária; Příhoda, Miroslav; Širillová, Ľubomíra

    2014-08-01

    This article gives the methodology for stating power losses and the efficiency of a gearbox working in natural cooling using dimensional analysis. The methodology reflects experimental research which investigated the effect of a change in revolutions and loading the gearbox on power losses. At the same time, it describes the conditions under which the proposed method may be applied for industrial, helical bevel geared motors.

  19. Cross-Sector Impact Analysis of Industrial Efficiency Measures

    SciTech Connect

    Morrow, William; CreskoEngineering, Joe; Carpenter, Alberta; Masanet, Eric; Nimbalkar, Sachin U; Shehabi, Arman

    2013-01-01

    The industrial or manufacturing sector is a foundational component to all economic activity. In addition to being a large direct consumer of energy, the manufacturing sector also produces materials, products, and technologies that influence the energy use of other economic sectors. For example, the manufacturing of a lighter-weight vehicle component affects the energy required to ship that component as well as the fuel efficiency of the assembled vehicle. Many energy efficiency opportunities exist to improve manufacturing energy consumption, however comparisons of manufacturing sector energy efficiency investment opportunities tend to exclude any impacts that occur once the product leaves the factory. Expanding the scope of analysis to include energy impacts across different stages of product life-cycle can highlight less obvious opportunities and inform actions that create the greatest economy-wide benefits. We present a methodology and associated analysis tool (LIGHTEnUP Lifecycle Industry GHgas, Technology and Energy through the Use Phase) that aims to capture both the manufacturing sector energy consumption and product life-cycle energy consumption implications of manufacturing innovation measures. The tool architecture incorporates U.S. national energy use data associated with manufacturing, building operations, and transportation. Inputs for technology assessment, both direct energy saving to the manufacturing sector, and indirect energy impacts to additional sectors are estimated through extensive literature review and engineering methods. The result is a transparent and uniform system of comparing manufacturing and use-phase impacts of technologies.

  20. Implementation and Rejection of Industrial Steam System Energy Efficiency Measures

    SciTech Connect

    Therkelesen, Peter; McKane, Aimee

    2013-05-01

    Steam systems consume approximately one third of energy applied at U.S. industrial facilities. To reduce energy consumption, steam system energy assessments have been conducted on a wide range of industry types over the course of five years through the Energy Savings Assessment (ESA) program administered by the U.S. Department of Energy (U.S. DOE). ESA energy assessments result in energy efficiency measure recommendations that are given potential energy and energy cost savings and potential implementation cost values. Saving and cost metrics that measure the impact recommended measures will have at facilities, described as percentages of facility baseline energy and energy cost, are developed from ESA data and used in analyses. Developed savings and cost metrics are examined along with implementation and rejection rates of recommended steam system energy efficiency measures. Based on analyses, implementation of steam system energy efficiency measures is driven primarily by cost metrics: payback period and measure implementation cost as a percentage of facility baseline energy cost (implementation cost percentage). Stated reasons for rejecting recommended measures are primarily based upon economic concerns. Additionally, implementation rates of measures are not only functions of savings and cost metrics, but time as well.

  1. Restructuring, ownership and efficiency in the electricity industry

    NASA Astrophysics Data System (ADS)

    Shanefelter, Jennifer Kaiser

    The first chapter considers improvements in productive efficiency that can result from a movement from a regulated framework to one that allows for market-based incentives for industry participants. Specifically, I look at the case of restructuring in the electricity generation industry. Using data from the electricity industry, this analysis considers the total effect of restructuring on one input to the production process, labor, as reflected in employment levels, payroll per employee and aggregate establishment payroll. Using concurrent payroll and employment data from non-utility ("merchant") and utility generators in both restructured and nonrestructured states, I estimate the effect of market liberalization, comprising both new entry and state-level legislation, on employment and payroll in this industry. I find that merchant owners of divested generation assets employ significantly fewer people, but that the payroll per employee is not significantly different from what workers at utility-owned plants are paid. As a result, the new merchant owners of these plants have significantly lower aggregate payroll expenses. Decomposing the effect into a merchant effect and a divestiture effect, I find that merchant ownership is the primary driver of these results. As documented in Chapter 1, merchant power plants have lower overall payroll costs than plants owned by utilities. Employment at merchant power plants is characterized by reduced staffing levels but higher average payroll per employee. A hypothesis set forth in that paper is that merchant generators employ fewer workers at the lower end of the wage distribution, resulting in a higher average payroll per employee. The second chapter of this paper examines whether employment at nonutility power plants, that is, those that are either divested or native merchant power plants, is skewed towards more skilled labor. This chapter also considers the extent to which the difference in employment levels is the result of

  2. Nanocoatings for High-Efficiency Industrial Hydraulic and Tooling Systems

    SciTech Connect

    Clifton B. Higdon III

    2011-01-07

    Industrial manufacturing in the U.S. accounts for roughly one third of the 98 quadrillion Btu total energy consumption. Motor system losses amount to 1.3 quadrillion Btu, which represents the largest proportional loss of any end-use category, while pumps alone represent over 574 trillion BTU (TBTU) of energy loss each year. The efficiency of machines with moving components is a function of the amount of energy lost to heat because of friction between contacting surfaces. The friction between these interfaces also contributes to downtime and the loss of productivity through component wear and subsequent repair. The production of new replacement parts requires additional energy. Among efforts to reduce energy losses, wear-resistant, low-friction coatings on rotating and sliding components offer a promising approach that is fully compatible with existing equipment and processes. In addition to lubrication, one of the most desirable solutions is to apply a protective coating or surface treatment to rotating or sliding components to reduce their friction coefficients, thereby leading to reduced wear. Historically, a number of materials such as diamond-like carbon (DLC), titanium nitride (TiN), titanium aluminum nitride (TiAlN), and tungsten carbide (WC) have been examined as tribological coatings. The primary objective of this project was the development of a variety of thin film nanocoatings, derived from the AlMgB14 system, with a focus on reducing wear and friction in both industrial hydraulics and cutting tool applications. Proof-of-concept studies leading up to this project had shown that the constituent phases, AlMgB14 and TiB2, were capable of producing low-friction coatings by pulsed laser deposition. These coatings combine high hardness with a low friction coefficient, and were shown to substantially reduce wear in laboratory tribology tests. Selection of the two applications was based largely on the concept of improved mechanical interface efficiencies for

  3. Coevolution of RAC Small GTPases and their Regulators GEF Proteins

    PubMed Central

    Jiménez-Sánchez, Alejandro

    2016-01-01

    RAC proteins are small GTPases involved in important cellular processes in eukaryotes, and their deregulation may contribute to cancer. Activation of RAC proteins is regulated by DOCK and DBL protein families of guanine nucleotide exchange factors (GEFs). Although DOCK and DBL proteins act as GEFs on RAC proteins, DOCK and DBL family members are evolutionarily unrelated. To understand how DBL and DOCK families perform the same function on RAC proteins despite their unrelated primary structure, phylogenetic analyses of the RAC, DBL, and DOCK families were implemented, and interaction patterns that may suggest a coevolutionary process were searched. Interestingly, while RAC and DOCK proteins are very well conserved in humans and among eukaryotes, DBL proteins are highly divergent. Moreover, correlation analyses of the phylogenetic distances of RAC and GEF proteins and covariation analyses between residues in the interacting domains showed significant coevolution rates for both RAC–DOCK and RAC–DBL interactions. PMID:27226705

  4. India's pulp and paper industry: Productivity and energy efficiency

    SciTech Connect

    Schumacher, Katja

    1999-07-01

    Historical estimates of productivity growth in India's pulp and paper sector vary from indicating an improvement to a decline in the sector's productivity. The variance may be traced to the time period of study, source of data for analysis, and type of indices and econometric specifications used for reporting productivity growth. The authors derive both statistical and econometric estimates of productivity growth for this sector. Their results show that productivity declined over the observed period from 1973-74 to 1993-94 by 1.1% p.a. Using a translog specification the econometric analysis reveals that technical progress in India's pulp and paper sector has been biased towards the use of energy and material, while it has been capital and labor saving. The decline in productivity was caused largely by the protection afforded by high tariffs on imported paper products and other policies, which allowed inefficient, small plants to enter the market and flourish. Will these trends continue into the future, particularly where energy use is concerned? The authors examine the current changes in structure and energy efficiency undergoing in the sector. Their analysis shows that with liberalization of the sector, and tighter environmental controls, the industry is moving towards higher efficiency and productivity. However, the analysis also shows that because these improvements are being hampered by significant financial and other barriers the industry might have a long way to go.

  5. Methods for assessing the energy-saving efficiency of industrial symbiosis in industrial parks.

    PubMed

    Li, Wenfeng; Cui, Zhaojie; Han, Feng

    2015-01-01

    The available energy resources are being depleted worldwide. Industrial symbiosis (IS) provides a promising approach for increasing the efficiency of energy utilization, with numerous studies reporting the superiority of this technology. However, studies quantifying the energy-saving efficiency of IS remain insufficient. This paper proposes an index system for the quantitative evaluation of the energy-saving efficiency of IS. Both energy-saving and financial indexes were selected, the former include the IS energy-saving index, the contribution rate of energy saved through IS, fractional energy savings, and cut rate of energy consumption per total output value; and the latter include the IS investment payback period, IS input-output ratio, net present value (NPV), and internal rate of return (IRR) of IS. The proposed methods were applied to a case study on the XF Industrial Park (XF IP), in the city of Liaocheng in Shandong Province of China. Three energy-saving channels using IS were found in the XF IP: (a) utilizing the energy of high-temperature materials among industrial processes, (b) recovering waste heat and steam between different processes, and (c) saving energy by sharing infrastructures. The results showed that the energy efficiency index of IS was 0.326, accounting for 34.6% of the comprehensive energy-saving index in 2011, and the fractional energy-savings were 12.42%. The index of energy consumption per total industrial output value varied from 90.9 tce/MRMB to 51.6 tce/MRMB. Thus, the cut rate of energy consumption per total industrial output value was 43.42%. The average values of the IS input-output ratio was 406.2 RMB/tce, 57.2% lower than the price of standard coal. Static investment payback period in the XF IP was 8.5 months, indicating that the XF IP began to earn profit 8.5 months after the construction of all IS modes. The NVP and IRR of each IS mode in the XF IP were greater than zero, with average values equal to 1,789.96 MRMB and 140

  6. Developing an energy efficiency service industry in Shanghai

    SciTech Connect

    Lin, Jiang; Goldman, Charles; Levine, Mark; Hopper, Nicole

    2004-02-10

    The rapid development of the Chinese economy over the past two decades has led to significant growth in China's energy consumption and greenhouse gas (GHG) emissions. Between 1980 and 2000, China's energy consumption more than doubled from 602 million to 1.3 billion tons of coal-equivalent (NBS, 2003). In 2000, China's GHG emissions were about 12% of the global total, ranked second behind only the US. According to the latest national development plan issued by the Chinese government, China's energy demand is likely to double again by 2020 (DRC, 2004), based on a quadrupling of its gross domestic product (GDP). The objectives of the national development plan imply that China needs to significantly raise the energy efficiency of its economy, i.e., cutting the energy intensity of its economy by half. Such goals are extremely ambitious, but not infeasible. China has achieved such reductions in the past, and its current overall level of energy efficiency remains far behind those observed in other developed economies. However, challenges remain whether China can put together an appropriate policy framework and the institutions needed to improve the energy efficiency of its economy under a more market-based economy today. Shanghai, located at the heart of the Yangtze River Delta, is the most dynamic economic and financial center in the booming Chinese economy. With 1% of Chinese population (13 million inhabitants), its GDP in 2000 stood at 455 billion RMB yuan (5% of the national total), with an annual growth rate of 12%--much higher than the national average. It is a major destination for foreign as well as Chinese domestic investment. In 2003, Shanghai absorbed 10% of actual foreign investment in all China (''Economist'', January 17-23, 2004). Construction in Shanghai continues at a breakneck pace, with an annual addition of approximately 200 million square foot of residential property and 100 million square foot of commercial and industrial space over the last 5 years

  7. Restructuring, ownership and efficiency in the electricity industry

    NASA Astrophysics Data System (ADS)

    Shanefelter, Jennifer Kaiser

    The first chapter considers improvements in productive efficiency that can result from a movement from a regulated framework to one that allows for market-based incentives for industry participants. Specifically, I look at the case of restructuring in the electricity generation industry. Using data from the electricity industry, this analysis considers the total effect of restructuring on one input to the production process, labor, as reflected in employment levels, payroll per employee and aggregate establishment payroll. Using concurrent payroll and employment data from non-utility ("merchant") and utility generators in both restructured and nonrestructured states, I estimate the effect of market liberalization, comprising both new entry and state-level legislation, on employment and payroll in this industry. I find that merchant owners of divested generation assets employ significantly fewer people, but that the payroll per employee is not significantly different from what workers at utility-owned plants are paid. As a result, the new merchant owners of these plants have significantly lower aggregate payroll expenses. Decomposing the effect into a merchant effect and a divestiture effect, I find that merchant ownership is the primary driver of these results. As documented in Chapter 1, merchant power plants have lower overall payroll costs than plants owned by utilities. Employment at merchant power plants is characterized by reduced staffing levels but higher average payroll per employee. A hypothesis set forth in that paper is that merchant generators employ fewer workers at the lower end of the wage distribution, resulting in a higher average payroll per employee. The second chapter of this paper examines whether employment at nonutility power plants, that is, those that are either divested or native merchant power plants, is skewed towards more skilled labor. This chapter also considers the extent to which the difference in employment levels is the result of

  8. GUIDE TO INDUSTRIAL ASSESSMENTS FOR POLLUTION PREVENTION AND ENERGY EFFICIENCY

    EPA Science Inventory

    This document presents an overview of industrial assessments and the general framework for conducting an assessment. It describes combined assessments for pollution prevention and energy, "industrial assessments," providing guidance to those performing assessments at industrial o...

  9. Tension on JAM-A activates RhoA via GEF-H1 and p115 RhoGEF

    PubMed Central

    Scott, David W.; Tolbert, Caitlin E.; Burridge, Keith

    2016-01-01

    Junctional adhesion molecule A (JAM-A) is a broadly expressed adhesion molecule that regulates cell–cell contacts and facilitates leukocyte transendothelial migration. The latter occurs through interactions with the integrin LFA-1. Although we understand much about JAM-A, little is known regarding the protein’s role in mechanotransduction or as a modulator of RhoA signaling. We found that tension imposed on JAM-A activates RhoA, which leads to increased cell stiffness. Activation of RhoA in this system depends on PI3K-mediated activation of GEF-H1 and p115 RhoGEF. These two GEFs are further regulated by FAK/ERK and Src family kinases, respectively. Finally, we show that phosphorylation of JAM-A at Ser-284 is required for RhoA activation in response to tension. These data demonstrate a direct role of JAM-A in mechanosignaling and control of RhoA and implicate Src family kinases in the regulation of p115 RhoGEF. PMID:26985018

  10. Superoxide Inhibits Guanine Nucleotide Exchange Factor (GEF) Action on Ras, but not on Rho, through Desensitization of Ras to GEF

    PubMed Central

    2015-01-01

    Ras and Rho GTPases are molecular switches for various vital cellular signaling pathways. Overactivation of these GTPases often causes development of cancer. Guanine nucleotide exchange factors (GEFs) and oxidants function to upregulate these GTPases through facilitation of guanine nucleotide exchange (GNE) of these GTPases. However, the effect of oxidants on GEF functions, or vice versa, has not been known. We show that, via targeting Ras Cys51, an oxidant inhibits the catalytic action of Cdc25—the catalytic domain of RasGEFs—on Ras. However, the enhancement of Ras GNE by an oxidant continues regardless of the presence of Cdc25. Limiting RasGEF action by an oxidant may function to prevent the pathophysiological overactivation of Ras in the presence of both RasGEFs and oxidants. The continuous exposure of Ras to nitric oxide and its derivatives can form S-nitrosated Ras (Ras-SNO). This study also shows that an oxidant not only inhibits the catalytic action of Cdc25 on Ras-SNO but also fails to enhance Ras-SNO GNE. This lack of enhancement then populates the biologically inactive Ras-SNO in cells, which may function to prevent the continued redox signaling of the Ras pathophysiological response. Finally, this study also demonstrates that, unlike the case with RasGEFs, an oxidant does not inhibit the catalytic action of RhoGEF—Vav or Dbs—on Rho GTPases such as Rac1, RhoA, RhoC, and Cdc42. This result explains the results of the previous study in which, despite the presence of an oxidant, the catalytic action of Dbs in cells continued to enhance RhoC GNE. PMID:24422478

  11. Energy Efficiency Improvement Opportunities for the Cement Industry

    SciTech Connect

    Price, Lynn; Worrell, Ernst; Galitsky, Christina; Price, Lynn

    2008-01-31

    This report provides information on the energy savings, costs, and carbon dioxide emissions reductions associated with implementation of a number of technologies and measures applicable to the cement industry. The technologies and measures include both state-of-the-art measures that are currently in use in cement enterprises worldwide as well as advanced measures that are either only in limited use or are near commercialization. This report focuses mainly on retrofit measures using commercially available technologies, but many of these technologies are applicable for new plants as well. Where possible, for each technology or measure, costs and energy savings per tonne of cement produced are estimated and then carbon dioxide emissions reductions are calculated based on the fuels used at the process step to which the technology or measure is applied. The analysis of cement kiln energy-efficiency opportunities is divided into technologies and measures that are applicable to the different stages of production and various kiln types used in China: raw materials (and fuel) preparation; clinker making (applicable to all kilns, rotary kilns only, vertical shaft kilns only); and finish grinding; as well as plant wide measures and product and feedstock changes that will reduce energy consumption for clinker making. Table 1 lists all measures in this report by process to which they apply, including plant wide measures and product or feedstock changes. Tables 2 through 8 provide the following information for each technology: fuel and electricity savings per tonne of cement; annual operating and capital costs per tonne of cement or estimated payback period; and, carbon dioxide emissions reductions for each measure applied to the production of cement. This information was originally collected for a report on the U.S. cement industry (Worrell and Galitsky, 2004) and a report on opportunities for China's cement kilns (Price and Galitsky, in press). The information provided in this

  12. Two essays on efficiency in the electric power industry: Measurement of technical and allocative efficiency

    NASA Astrophysics Data System (ADS)

    Gardiner, John Corby

    The electric power industry market structure has changed over the last twenty years since the passage of the Public Utility Regulatory Policies Act (PURPA). These changes include the entry by unregulated generator plants and, more recently, the deregulation of entry and price in the retail generation market. Such changes have introduced and expanded competitive forces on the incumbent electric power plants. Proponents of this deregulation argued that the enhanced competition would lead to a more efficient allocation of resources. Previous studies of power plant technical and allocative efficiency have failed to measure technical and allocative efficiency at the plant level. In contrast, this study uses panel data on 35 power plants over 59 years to estimate technical and allocative efficiency of each plant. By using a flexible functional form, which is not constrained by the assumption that regulation is constant over the 59 years sampled, the estimation procedure accounts for changes in both state and national regulatory/energy policies that may have occurred over the sample period. The empirical evidence presented shows that most of the power plants examined have operated more efficiently since the passage of PURPA and the resultant increase of competitive forces. Chapter 2 extends the model used in Chapter 1 and clarifies some issues in the efficiency literature by addressing the case where homogeneity does not hold. A more general model is developed for estimating both input and output inefficiency simultaneously. This approach reveals more information about firm inefficiency than the single estimation approach that has previously been used in the literature. Using the more general model, estimates are provided on the type of inefficiency that occurs as well as the cost of inefficiency by type of inefficiency. In previous studies, the ranking of firms by inefficiency has been difficult because of the cardinal and ordinal differences between different types of

  13. Linking Energy Efficiency and ISO: Creating a Framework forSustainable Industrial Energy Efficiency

    SciTech Connect

    McKane, Aimee; Perry, Wayne; Aixian, Li; Tienan, Li; Williams,Robert

    2005-04-01

    Industrial motor-driven systems consume more than 2194billion kWh annually on a global basis and offer one of the largestopportunities for energy savings. In the United States (US), they accountfor more than 50 percent of all manufacturing electricity use. Incountries with less well-developed consumer economies, the proportion ofelectricity consumed by motors is higher-more than 50 percent ofelectricity used in all sectors in China is attributable to motors.Todate, the energy savings potential from motor-driven systems haveremained largely unrealized worldwide. Both markets and policy makerstend to focus on individual system components, which have a typicalimprovement potential of 2-5 percent versus 20-50 percent for completesystems. Several factors contribute to this situation, most notably thecomplexity of the systems themselves. Determining how to optimize asystem requires a high level of technical skill. In addition, once anenergy efficiency project is completed, the energy savings are often notsustained due to changes in personnel and production processes. Althoughtraining and educational programs in the US, UK, and China to promotesystem optimization have proven effective, these resource-intensiveefforts have only reached a small portion of the market.The same factorsthat make it so challenging to achieve and sustain energy efficiency inmotor-driven systems (complexity, frequent changes) apply to theproduction processes that they support. Yet production processestypically operate within a narrow band of acceptable performance. Theseprocesses are frequently incorporated into ISO 9000/14000 quality andenvironmental management systems, which require regular, independentaudits to maintain ISO certification, an attractive value forinternational trade.This paper presents a new approach to achievingindustrial system efficiency (motors and steam) that will encourageplants to incorporate system energy efficiency into their existing ISOmanagement systems. We will

  14. 75 FR 59657 - Energy Efficiency Program for Certain Commercial and Industrial Equipment: Public Meeting and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-28

    ... Industrial Equipment: Public Meeting and Availability of the Framework Document for Commercial and Industrial... commercial and industrial electric motors under section 342(b) of the Energy Policy and Conservation Act... energy efficiency of certain commercial and industrial equipment, including the electric motors that...

  15. 3 CFR 13624 - Executive Order 13624 of August 30, 2012. Accelerating Investment in Industrial Energy Efficiency

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    .... Accelerating Investment in Industrial Energy Efficiency 13624 Order 13624 Presidential Documents Executive... energy efficiency at industrial facilities, it is hereby ordered as follows: Section 1. Policy. The... energy efficiency and CHP as a result of numerous barriers. The Federal Government has limited...

  16. The Office of Industrial Technologies - enhancing the competitiveness, efficiency, and environmental quality of American industry through technology partnerships

    SciTech Connect

    1997-09-01

    A critical component of the Federal Government`s effort to stimulate improved industrial energy efficiency is the DOE`s Office of Industrial Technologies (OIT). OIT funds research, development, and demonstration (RD&D) efforts and transfers the resulting technology and knowledge to industry. This document describes OIT`s program, including the new Industries of the Future (IOF) initiative and the strategic activities that are part of the IOF process. It also describes the energy, economic, and environmental characteristics of the materials and process industries that consume nearly 80% of all energy used by manufacturing in the United States. OIT-supported RD&D activities relating to these industries are described, and quantitative estimates of the potential benefits of many OIT-supported technologies for industry are also provided.

  17. 78 FR 28812 - Energy Efficiency Program for Industrial Equipment: Petition of UL Verification Services Inc. for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-16

    ... national recognition of an energy efficiency certification program for ] electric motors. See 77 FR 26608... of Energy Efficiency and Renewable Energy Energy Efficiency Program for Industrial Equipment... Program for Small Electric Motors AGENCY: Office of Energy Efficiency and Renewable Energy, Department...

  18. GEF climate change operational strategy: Whither UNDP?

    SciTech Connect

    Hosier, R.

    1996-12-31

    The paper discusses aspects of the implementation of the program for climatic change which has been come about as part of the U.N. Framework Convention on Climate Change. Initial effort has focused on providing strategic information and help to countries, on achieving offsets in greenhouse gas emissions by energy conservation or carbon sinking, and an emphasis on development of renewable energy supplies. The U.N. Development Agency has limited funding to help support startup on projects submitted. Specific examples are discussed in the areas of energy conservation and energy efficiency, adoption of renewable energy sources, and reducing the long-term costs of low greenhouse gas-emitting energy technologies.

  19. Characteristic occurrence patterns of micropollutants and their removal efficiencies in industrial wastewater treatment plants.

    PubMed

    Lee, In-Seok; Sim, Won-Jin; Kim, Chang-Won; Chang, Yoon-Seok; Oh, Jeong-Eun

    2011-02-01

    The concentrations and removal efficiencies of various kinds of micropollutants were investigated and the relationships between the input sources of industrial wastewater and occurrence patterns of each micropollutant were identified at nine on-site industrial wastewater treatment plants (WWTPs). The distribution pattern of each compound varied according to the WWTP type and several micropollutants were significantly related with specific industries: chlorinated phenols (ClPhs) with paper and metal industries, polycyclic aromatic hydrocarbons (PAHs) with petrogenic- and pyrogenic-related industries, polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) with the paper industry, and chlorinated benzenes (ClBzs) with dye-related industries. The activated sludge (AS) process was very efficient in the removal of ClPhs and PAHs, and the filtration process in the removal of PCDD/Fs and 1,4-dioxane. Generally, the removal efficiencies of each micropollutant varied according to the WWTP type. PMID:21140016

  20. The RAB5-GEF Function of RIN1 Regulates Multiple Steps During Listeria monocytogenes Infection

    PubMed Central

    Balaji, Kavitha; French, Christopher T.; Miller, Jeff F.; Colicelli, John

    2014-01-01

    Listeria monocytogenes is a food-borne pathogenic bacterium that invades intestinal epithelial cells through a phagocytic pathway that relies on activation of host cell RAB5 GTPases. L. monocytogenes must subsequently inhibit RAB5, however, in order to escape lysosome-mediated destruction. Relatively little is known about upstream RAB5 regulators during L. monocytogenes entry and phagosome escape processes in epithelial cells. Here we identify RIN1, a RAS effector and RAB5-directed GEF, as a host cell factor in L. monocytogenes infection. RIN1 is rapidly engaged following L. monocytogenes infection and is required for efficient invasion of intestinal epithelial cells. RIN1-mediated RAB5 activation later facilitates the fusion of phagosomes with lysosomes, promoting clearance of bacteria from the host cell. These results suggest that RIN1 is a host cell regulator that performs counterbalancing functions during early and late stages of L. monocytogenes infection, ultimately favoring pathogen clearance. PMID:25082076

  1. Rab3-GEF Controls Active Zone Development at the Drosophila Neuromuscular Junction123

    PubMed Central

    Bae, Haneui; Chen, Shirui; Roche, John P.; Ai, Minrong; Wu, Chunlai

    2016-01-01

    Abstract Synaptic signaling involves the release of neurotransmitter from presynaptic active zones (AZs). Proteins that regulate vesicle exocytosis cluster at AZs, composing the cytomatrix at the active zone (CAZ). At the Drosophila neuromuscular junction (NMJ), the small GTPase Rab3 controls the distribution of CAZ proteins across release sites, thereby regulating the efficacy of individual AZs. Here we identify Rab3-GEF as a second protein that acts in conjunction with Rab3 to control AZ protein composition. At rab3-GEF mutant NMJs, Bruchpilot (Brp) and Ca2+ channels are enriched at a subset of AZs, leaving the remaining sites devoid of key CAZ components in a manner that is indistinguishable from rab3 mutant NMJs. As the Drosophila homologue of mammalian DENN/MADD and Caenorhabditis elegans AEX-3, Rab3-GEF is a guanine nucleotide exchange factor (GEF) for Rab3 that stimulates GDP to GTP exchange. Mechanistic studies reveal that although Rab3 and Rab3-GEF act within the same mechanism to control AZ development, Rab3-GEF is involved in multiple roles. We show that Rab3-GEF is required for transport of Rab3. However, the synaptic phenotype in the rab3-GEF mutant cannot be fully explained by defective transport and loss of GEF activity. A transgenically expressed GTP-locked variant of Rab3 accumulates at the NMJ at wild-type levels and fully rescues the rab3 mutant but is unable to rescue the rab3-GEF mutant. Our results suggest that although Rab3-GEF acts upstream of Rab3 to control Rab3 localization and likely GTP-binding, it also acts downstream to regulate CAZ development, potentially as a Rab3 effector at the synapse. PMID:27022630

  2. Impact of information technology on productivity and efficiency in Iranian manufacturing industries

    NASA Astrophysics Data System (ADS)

    Abri, Amir Gholam; Mahmoudzadeh, Mahmoud

    2015-12-01

    The aim of this paper is to assess the impact of information technology (IT) on the productivity and efficiency of manufacturing industries in Iran. So, the data will be collected from 23 Iranian manufacturing industries during "2002-2006" and the methods such as DEA and panel data used to study the subject. Results obtained by the above two methods represent that IT has a positive and statistically significant effect on the productivity of manufacturing industries. It will be more in high IT-intensive industries than the other industries. But, there is no significant difference between the growth of labor productivity in IT-producing and IT-using industries.

  3. Nanocoatings for High-Efficiency Industrial Hydraulic and Tooling Systems

    SciTech Connect

    2009-05-01

    This factsheet describes a research project whose goal is to develop degradation-resistant nano-coatings of AlMgB14 and AlMgB14– (titanium diboride) TiB2 that result in improved surface hardness and reduced friction for industrial hydraulic and tooling systems.

  4. Premium Efficiency Motor Selection and Application Guide – A Handbook for Industry

    SciTech Connect

    Gilbert A. McCoy and John G. Douglass

    2014-02-01

    This handbook informs new motor purchase decisions by identifying energy and cost savings that can come from replacing motors with premium efficiency units. The handbook provides an overview of current motor use in the industrial sector, including the development of motor efficiency standards, currently available and emerging advanced efficiency motor technologies, and guidance on how to evaluate motor efficiency opportunities. It also several tips on getting the most out of industrial motors, such as how to avoid adverse motor interactions with electronic adjustable speed drives and how to ensure efficiency gains are not lost to undervoltage operation or excessive voltage unbalance.

  5. Performance analysis of CO(2) emissions and energy efficiency of metal industries in China.

    PubMed

    Shao, Chaofeng; Guan, Yang; Wan, Zheng; Chu, Chunli; Ju, Meiting

    2014-02-15

    Nonferrous metal industries play an important role in China's national economy and are some of the country's largest energy consumers. To better understand the nature of CO(2) emissions from these industries and to further move towards low-carbon development in this industry sector, this study investigates the CO(2) emissions of 12 nonferrous metal industries from 2003 to 2010 based on their life-cycle assessments. It then classifies these industries into four "emission-efficiency" types through cluster analysis. The results show that (1) the industrial economy and energy consumption of China's nonferrous metal industries have grown rapidly, although their recent energy consumption rate shows a declining trend. (2) The copper, aluminum, zinc, lead, and magnesium industries, classified as high-emission industries, are the main contributors of CO(2) emissions. The results have implications for policy decisions that aim to enhance energy efficiency, particularly for promoting the transformation of low-efficiency industries to high-efficiency ones. The study also highlights the important role of policy development in technological innovations, optimization, and upgrades, the reduction of coal proportion in energy consumption, and the advancement of new energy sources. PMID:24463733

  6. Phosphorylation-dependent inhibition of Cdc42 GEF Gef1 by 14-3-3 protein Rad24 spatially regulates Cdc42 GTPase activity and oscillatory dynamics during cell morphogenesis

    PubMed Central

    Das, Maitreyi; Nuñez, Illyce; Rodriguez, Marbelys; Wiley, David J.; Rodriguez, Juan; Sarkeshik, Ali; Yates, John R.; Buchwald, Peter; Verde, Fulvia

    2015-01-01

    Active Cdc42 GTPase, a key regulator of cell polarity, displays oscillatory dynamics that are anticorrelated at the two cell tips in fission yeast. Anticorrelation suggests competition for active Cdc42 or for its effectors. Here we show how 14-3-3 protein Rad24 associates with Cdc42 guanine exchange factor (GEF) Gef1, limiting Gef1 availability to promote Cdc42 activation. Phosphorylation of Gef1 by conserved NDR kinase Orb6 promotes Gef1 binding to Rad24. Loss of Rad24–Gef1 interaction increases Gef1 protein localization and Cdc42 activation at the cell tips and reduces the anticorrelation of active Cdc42 oscillations. Increased Cdc42 activation promotes precocious bipolar growth activation, bypassing the normal requirement for an intact microtubule cytoskeleton and for microtubule-dependent polarity landmark Tea4-PP1. Further, increased Cdc42 activation by Gef1 widens cell diameter and alters tip curvature, countering the effects of Cdc42 GTPase-activating protein Rga4. The respective levels of Gef1 and Rga4 proteins at the membrane define dynamically the growing area at each cell tip. Our findings show how the 14-3-3 protein Rad24 modulates the availability of Cdc42 GEF Gef1, a homologue of mammalian Cdc42 GEF DNMBP/TUBA, to spatially control Cdc42 GTPase activity and promote cell polarization and cell shape emergence. PMID:26246599

  7. Feed Efficiency: An Assessment of Current Knowledge from a Voluntary Subsample of the Swine Industry

    ERIC Educational Resources Information Center

    Flohr, Josh R.; Tokach, Mike D.; DeRouchey, Joel M.; Goodband, Robert D.; Dritz, Steve S.; Nelssen, Jim L.; Patience, John F.

    2014-01-01

    A voluntary sample of pork producers and advisers to the swine industry were surveyed about feed efficiency. The questionnaire was designed to accomplish three objectives: (a) determine the level of knowledge related to feed efficiency topics, (b) identify production practices used that influence feed efficiency, and (c) identify information gaps…

  8. 78 FR 54197 - Energy Efficiency Program for Commercial and Industrial Equipment: Energy Conservation Standards...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-03

    ...; ] DEPARTMENT OF ENERGY 10 CFR Part 431 RIN 1904-AD01 Energy Efficiency Program for Commercial and Industrial Equipment: Energy Conservation Standards for Commercial Packaged Boilers AGENCY: Office of Energy Efficiency... Efficiency and Renewable Energy, Building Technologies Office, EE-2J, 1000 Independence Avenue...

  9. General Description of Fission Observables: GEF Model Code

    NASA Astrophysics Data System (ADS)

    Schmidt, K.-H.; Jurado, B.; Amouroux, C.; Schmitt, C.

    2016-01-01

    The GEF ("GEneral description of Fission observables") model code is documented. It describes the observables for spontaneous fission, neutron-induced fission and, more generally, for fission of a compound nucleus from any other entrance channel, with given excitation energy and angular momentum. The GEF model is applicable for a wide range of isotopes from Z = 80 to Z = 112 and beyond, up to excitation energies of about 100 MeV. The results of the GEF model are compared with fission barriers, fission probabilities, fission-fragment mass- and nuclide distributions, isomeric ratios, total kinetic energies, and prompt-neutron and prompt-gamma yields and energy spectra from neutron-induced and spontaneous fission. Derived properties of delayed neutrons and decay heat are also considered. The GEF model is based on a general approach to nuclear fission that explains a great part of the complex appearance of fission observables on the basis of fundamental laws of physics and general properties of microscopic systems and mathematical objects. The topographic theorem is used to estimate the fission-barrier heights from theoretical macroscopic saddle-point and ground-state masses and experimental ground-state masses. Motivated by the theoretically predicted early localisation of nucleonic wave functions in a necked-in shape, the properties of the relevant fragment shells are extracted. These are used to determine the depths and the widths of the fission valleys corresponding to the different fission channels and to describe the fission-fragment distributions and deformations at scission by a statistical approach. A modified composite nuclear-level-density formula is proposed. It respects some features in the superfluid regime that are in accordance with new experimental findings and with theoretical expectations. These are a constant-temperature behaviour that is consistent with a considerably increased heat capacity and an increased pairing condensation energy that is

  10. High Efficiency Microwave Power Amplifier: From the Lab to Industry

    NASA Technical Reports Server (NTRS)

    Sims, William Herbert, III; Bell, Joseph L. (Technical Monitor)

    2001-01-01

    Since the beginnings of space travel, various microwave power amplifier designs have been employed. These included Class-A, -B, and -C bias arrangements. However, shared limitation of these topologies is the inherent high total consumption of input power associated with the generation of radio frequency (RF)/microwave power. The power amplifier has always been the largest drain for the limited available power on the spacecraft. Typically, the conversion efficiency of a microwave power amplifier is 10 to 20%. For a typical microwave power amplifier of 20 watts, input DC power of at least 100 watts is required. Such a large demand for input power suggests that a better method of RF/microwave power generation is required. The price paid for using a linear amplifier where high linearity is unnecessary includes higher initial and operating costs, lower DC-to-RF conversion efficiency, high power consumption, higher power dissipation and the accompanying need for higher capacity heat removal means, and an amplifier that is more prone to parasitic oscillation. The first use of a higher efficiency mode of power generation was described by Baxandall in 1959. This higher efficiency mode, Class-D, is achieved through distinct switching techniques to reduce the power losses associated with switching, conduction, and gate drive losses of a given transistor.

  11. Ultrasound in the meat industry: general applications and decontamination efficiency.

    PubMed

    Turantaş, Fulya; Kılıç, Gülden Başyiğit; Kılıç, Birol

    2015-04-01

    This review summarizes the findings of research focused on ultrasound as a "green", nonchemical technology in the meat industry to improve meat quality and safety. An overview of the importance of the decontamination in meat processing and microbial inactivation using ultrasound combined with some other applications is provided along with results of high power ultrasound studies which have been applied and adapted in the meat industry. The research results revealed that ultrasound by itself or in combination with other processing and/or preservation methods has a potential for improving the general quality, marination and tenderness of meat, preventing microbial growth and recontamination in meat and meat products as well as for the determination of defects in carcasses and cleaning process equipment. This review will provide an interpretation of ultrasound applications, an up-to-date summary of published articles, and an overview of the microbial inactivation in meat and poultry and their products by ultrasound. Since there is a need for not only a pathogen-free product but also a quality product; this review also can be accepted as a report on the results of research in the field of meat quality improvements with ultrasound applications. PMID:25613122

  12. 78 FR 79423 - Energy Efficiency Program for Industrial Equipment: Petition of CSA Group for Classification as a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-30

    ... recognition of an energy efficiency certification program for electric motors. See 77 FR 26608, 26629... of Energy Efficiency and Renewable Energy Energy Efficiency Program for Industrial Equipment... Electric Motors AGENCY: Office of Energy Efficiency and Renewable Energy, Department of Energy....

  13. Industry Stakeholder Recommendations for DOE's RD&D for Increasing Energy Efficiency in Existing Homes

    SciTech Connect

    Plympton, P.; Dagher, L.; Zwack, B.

    2007-06-01

    This technical report documents feedback for Industry Stakeholders on the direction of future U.S. Department of Energy (DOE) research and development in the area of improving energy efficiency in existing residential buildings.

  14. Market impact of selected industrial energy efficient technologies. Final report Jun 81-May 82

    SciTech Connect

    Lerner, M.O.; Salama, S.; Kothari, V.S.

    1982-09-01

    This analysis is designed to assist the Gas Research Institute (GRI) in evaluating technologies within the Industrial Utilization (IU) program. The main focus of the study is to assess the market impacts of selected projects designed by GRI to improve industrial sector energy efficiency and reduce energy-related cost. Market impacts examined for GRI include the energy savings and consumer savings that could result from these industrial technologies sponsored by GRI.

  15. India's aluminum industry: Productivity, energy efficiency and carbon emissions

    SciTech Connect

    Schumacher, Katja; Sathaye, Jayant

    1999-07-01

    Historical estimates of productivity growth in India's aluminum sector vary from indicating an improvement to a decline in the sector's productivity. The variance may be traced to the time period of study, source of data for analysis, and type of indices and econometric specifications used for reporting productivity growth. An analysis shows that in the twenty year period, 1973 to 1993, productivity in the aluminum sector declined slightly by 0.2%. An econometric analysis reveals that technical progress in India's aluminum sector has been biased towards the use of energy, while it has been labor saving. The decline in productivity was mainly driven by a decline in the 1970s when capacity utilization was low and the energy crisis hit India and the world. From the early 1980s on productivity recuperated. The authors examine the current changes in structure and energy efficiency in the sector. Their analysis shows that the Indian aluminum sector has high potential to move towards world-best technology, which will result in fewer carbon emissions and more efficient energy use. Substantial energy savings and carbon reduction options exist.

  16. India's cement industry: Productivity, energy efficiency and carbon emissions

    SciTech Connect

    Schumacher, Katja; Sathaye, Jayant

    1999-07-01

    Historical estimates of productivity growth in India's cement sector vary from indicating an improvement to a decline in the sector's productivity. The variance may be traced to the time period of study, source of data for analysis, and type of indices and econometric specifications used for reporting productivity growth. Analysis shows that in the twenty year period, 1973 to 1993, productivity in the aluminum sector increased by 0.8% per annum. An econometric analysis reveals that technical progress in India's cement sector has been biased towards the use of energy and capital, while it has been material and labor saving. The increase in productivity was mainly driven by a period of progress between 1983 and 1991 following partial decontrol of the cement sector in 1982. The authors examine the current changes in structure and energy efficiency in the sector. Their analysis shows that the Indian cement sector is moving towards world-best technology, which will result in fewer carbon emissions and more efficient energy use. However, substantial further energy savings and carbon reduction potentials still exist.

  17. Investigation of energy efficiency in the electroplating industry: Final report

    SciTech Connect

    Not Available

    1987-01-01

    The electroplating industry is heavily dependent on electricity for process use. This problem is compounded by the fact that a large concentration of electroplaters in New York State are located in the New York City area, where local utility rates are high. To understand energy consumption of electroplaters, 15 energy surveys were performed to see if any energy usage trends exist and what subsequent methods could reduce them. Through the surveys it was established that most electroplaters could reduce their energy bills by performing minimal repairs such as replacing faulty steam traps and repairing steam leaks. To achieve greater energy savings would require additional equipment such as a demand controller for exhaust fans, better controls for heating solution tanks or insulate heated solution tanks. To achieve energy savings of 30% or more, major capital equipment would be required. Two technologies recognized for this purpose are computer control of the process equipment and cogeneration. Both methods would require modification to fit the electroplaters' needs, but remain as viable candidates.

  18. Plasma membrane restricted RhoGEF activity is sufficient for RhoA-mediated actin polymerization

    PubMed Central

    van Unen, Jakobus; Reinhard, Nathalie R.; Yin, Taofei; Wu, Yi I.; Postma, Marten; Gadella, Theodorus W.J.; Goedhart, Joachim

    2015-01-01

    The small GTPase RhoA is involved in cell morphology and migration. RhoA activity is tightly regulated in time and space and depends on guanine exchange factors (GEFs). However, the kinetics and subcellular localization of GEF activity towards RhoA are poorly defined. To study the mechanism underlying the spatiotemporal control of RhoA activity by GEFs, we performed single cell imaging with an improved FRET sensor reporting on the nucleotide loading state of RhoA. By employing the FRET sensor we show that a plasma membrane located RhoGEF, p63RhoGEF, can rapidly activate RhoA through endogenous GPCRs and that localized RhoA activity at the cell periphery correlates with actin polymerization. Moreover, synthetic recruitment of the catalytic domain derived from p63RhoGEF to the plasma membrane, but not to the Golgi apparatus, is sufficient to activate RhoA. The synthetic system enables local activation of endogenous RhoA and effectively induces actin polymerization and changes in cellular morphology. Together, our data demonstrate that GEF activity at the plasma membrane is sufficient for actin polymerization via local RhoA signaling. PMID:26435194

  19. The impact of energy prices on industrial energy efficiency and productivity

    SciTech Connect

    Boyd, G.A.

    1993-11-01

    Energy prices moved into the forefront of concern in the mid and late seventies when two oil price shocks drove up energy prices dramatically. The analysis of the subsequent increase in industrial energy efficiency, i.e., decline in energy use per unit of industrial output, has filled volumes of government and private studies. Despite the volumes of analysis, there remains no consensus on the magnitude of the effect of energy prices on industrial energy efficiency or the effect of the change in energy prices on productivity. This paper examines some sources of the controversy to initiate a dialog between policy makers, analysts, and the energy consumers and producers.

  20. Nanocoatings for High-Efficiency Industrial and Tooling Systems

    SciTech Connect

    Blau, P; Qu, J.; Higdon, C.

    2011-02-01

    This industry-driven project was the result of a successful response by Eaton Corporation to a DOE/ITP Program industry call. It consisted of three phases in which ORNL participated. In addition to Eaton Corporation and ORNL (CRADA), the project team included Ames Laboratory, who developed the underlying concept for aluminum-magnesium-boron based nanocomposite coatings [1], and Greenleaf, a small tooling manufacturer in western Pennsylvania. This report focuses on the portion of this work that was conducted by ORNL in a CRADA with Eaton Corporation. A comprehensive final report for the entire effort, which ended in September 2010, has been prepared by Eaton Corporation. Phase I, “Proof of Concept” ran for one year (September 1, 2006 to September 30, 2007) during which the applicability of AlMgB14 single-phase and nanocomposite coatings on hydraulic material coupons and components as well as on tool inserts was demonstrated.. The coating processes used either plasma laser deposition (PLD) or physical vapor deposition (PVD). During Phase I, ORNL conducted laboratory-scale pin-on-disk and reciprocating pin-on-flat tests of coatings produced by PLD and PVD. Non-coated M2 tool steel was used as a baseline for comparison, and the material for the sliding counterface was Type 52100 bearing steel since it simulated the pump materials. Initial tests were run mainly in a commercial hydraulic fluid named Mobil DTE-24, but some tests were later run in a water-glycol mixture as well. A tribosystem analysis was conducted to define the operating conditions of pump components and to help develop simulative tests in Phase II. Phase II, “Coating Process Scale-up” was intended to use scaled-up process to generate prototype parts. This involved both PLD practices at Ames Lab, and a PVD scale-up study at Eaton using its production capable equipment. There was also a limited scale-up study at Greenleaf for the tooling application. ORNL continued to conduct friction and wear

  1. EVALUATION OF THE EFFICIENCY OF INDUSTRIAL FLARES: INFLUENCE OF GAS COMPOSITION

    EPA Science Inventory

    The report gives results of a pilot-scale evaluation of the efficiency of industrial flares. The work (1) evaluated the effects of additional gas mixtures on flare stability and efficiency with and without pilot assist and (2) correlated flame stability for the different gas mixt...

  2. Advanced Nanostructured Molecular Sieves for Energy Efficient Industrial Separations

    SciTech Connect

    Kunhao Li, Michael Beaver

    2012-01-18

    Due to the very small relative volatility difference between propane and propylene, current propane/propylene separation by distillation requires very tall distillation towers (150-250 theoretical plates) and large reflux ratios (up to 15), which is considered to be the most energy consuming large-scale separation process. Adsorptive separation processes are widely considered to be more energy-efficient alternatives to distillation. However, slow diffusion kinetics/mass transport rate through the adsorbent bed often limits the performance of such processes, so further improvements are possible if intra-particle mass transfer rates can be improved. Rive Technology, Inc. is developing and commercializing its proprietary mesoporous zeolite technology for catalysis and separation. With well-controlled intracrystalline mesoporosity, diffusion kinetics through such mesoporous zeolite based catalysts is much improved relative to conventional zeolites, leading to significantly better product selectivity. This 'proof-of-principle' project (DE-EE0003470) is intended to demonstrate that Rive mesoporous zeolite technology can be extended and applied in adsorptive propane/propylene separation and lead to significant energy saving compared to the current distillation process. In this project, the mesoporous zeolite Y synthesis technology was successfully extended to X and A zeolites that are more relevant to adsorbent applications. Mesoporosity was introduced to zeolite X and A for the first time while maintaining adequate adsorption capacity. Zeolite adsorbents were tested for liquid phase separation performance using a pulse flow test unit and the test results show that the separation selectivity of the mesoporous zeolite adsorbent is much closer to optimal for a Simulated Moving Bed (SMB) separation process and the enhanced mesoporosity lead to >100% increase of overall mass transport rate for propane and propylene. These improvements will significantly improve the

  3. Industrial innovations for tomorrow: Advances in industrial energy-efficiency technologies

    NASA Astrophysics Data System (ADS)

    1992-08-01

    Engineering Resources, Inc., is developing and testing a biological process for converting the waste gases into acetic acid. The process can operate at the atmospheric temperature and pressure of the carbon black waste gas stream and can selectively utilize the gas components to produce acetic acid. The technology should also be applicable to waste gases from the production of coke (used to make steel) and other industries as well.

  4. Expanding the Pool of Federal Policy Options to Promote Industrial Energy Efficiency

    SciTech Connect

    Brown, Dr. Marilyn Ann; Cox, Matthew; Jackson, Roderick K; Lapsa, Melissa Voss

    2011-01-01

    Improving the energy efficiency of industry is essential for maintaining the viability of domestic manufacturing, especially in a world economy where production is shifting to low-cost, less regulated developing countries. Numerous studies have shown the potential for significant cost-effective energy-savings in U.S. industries, but the realization of this potential is hindered by regulatory, information, workforce, and financial obstacles. This report evaluates seven federal policy options aimed at improving the energy efficiency of industry, grounded in an understanding of industrial decision-making and the barriers to efficiency improvements. Detailed analysis employs the Georgia Institute of Technology's version of the National Energy Modeling System and spreadsheet calculations, generating a series of benefit/cost metrics spanning private and public costs and energy bill savings, as well as air pollution benefits and the social cost of carbon. Two of the policies would address regulatory hurdles (Output-Based Emissions Standards and a federal Energy Portfolio Standard with Combined Heat and Power); three would help to fill information gaps and workforce training needs (the Superior Energy Performance program, Implementation Support Services, and a Small Firm Energy Management program); and two would tackle financial barriers (Tax Lien Financing and Energy-Efficient Industrial Motor Rebates). The social benefit-cost ratios of these policies appear to be highly favorable based on a range of plausible assumptions. Each of the seven policy options has an appropriate federal role, broad applicability across industries, utilizes readily available technologies, and all are administratively feasible.

  5. Industrial innovations for tomorrow: Advances in industrial energy-efficiency technologies

    SciTech Connect

    Not Available

    1992-07-01

    Stimulated by public demand and state and federal legislation, industry has begun to develop bio- and photo- degradable plastics. so far, however, none of these degradable plastics meets all of the criteria for success - adequate physical and mechanical properties for the desired use, cost-effectiveness, and 100% degradability. Polylactic acid (PLA) plastic is one degradable plastic that shows promise. It has the desired properties and is 100% degradable. However, PLA plastic made by conventional techniques is not cost effective. Made from lactic acid, which is typically made form petroleum using a very costly synthesis process. Lactic acid can also be made from carbohydrates (starches), found in food processing wastes such as potato wastes, cheese whey, and sorghum. Conversion of starch to simple sugars, and fermentation of these sugars can produce lactic acid.

  6. Industrial Energy-Efficiency Improvement Program. Annual report to the Congress and the President 1979

    SciTech Connect

    Not Available

    1980-12-01

    The industrial energy efficiency improvement program to accelerate market penetration of new and emerging industrial technologies and practices which will improve energy efficiency; encourage substitution of more plentiful domestic fuels; and enhance recovery of energy and materials from industrial waste streams is described. The role of research, development, and demonstration; technology implementation; the reporting program; and progress are covered. Specific reports from the chemicals and allied products; primary metals; petroleum and coal products; stone, clay, and glass, paper and allied products; food and kindred products; fabricated metals; transportation equipment; machinery (except electrical); textile mill products; rubber and miscellaneous plastics; electrical and electronic equipment; lumber and wood; and tobacco products are discussed. Additional data from voluntary submissions, a summary on progress in the utilization of recovered materials, and an analysis of industrial fuel mix are briefly presented. (MCW)

  7. Combining total energy and energy industrial center concepts to increase utilization efficiency of geothermal energy

    NASA Technical Reports Server (NTRS)

    Bayliss, B. P.

    1974-01-01

    Integrating energy production and energy consumption to produce a total energy system within an energy industrial center which would result in more power production from a given energy source and less pollution of the environment is discussed. Strong governmental support would be required for the crash drilling program necessary to implement these concepts. Cooperation among the federal agencies, power producers, and private industry would be essential in avoiding redundant and fruitless projects, and in exploiting most efficiently our geothermal resources.

  8. ENERGY EFFICIENCY OPPORTUNITIES IN THE U.S. PULP AND PAPER INDUSTRY

    SciTech Connect

    Kramer, Klaas Jan; Masanet, Eric; Worrell, Ernst

    2009-01-01

    The U.S. pulp and paper industry consumes over $7 billion worth of purchased fuels and electricity per year. Energy efficiency improvement is an important way to reduce these costs and to increase predictable earnings, especially in times of high energy price volatility. There are a variety of opportunities available at individual plants in the U.S. pulp and paper industry to reduce energy consumption in a cost-effective manner. This paper provides a brief overview of the U.S. EPA ENERGY STAR(R) for Industry energy efficiency guidebook (a.k.a. the"Energy Guide") for pulp and paper manufacturers. The Energy Guide discusses a wide range of energy efficiency practices and energy-efficient technologies that can be implemented at the component, process, facility, and organizational levels. Also provided is a discussion of the trends, structure, and energy consumption characteristics of the U.S. pulp and paper industry along with a description of the major process technologies used within the industry. Many energy efficiency measure descriptions include expected savings in energy and energy-related costs, based on case study data from real-world applications in pulp and paper mills and related industries worldwide. The information in this Energy Guide is intended to help energy and plant managers in the U.S. pulp and paper industry reduce energy consumption in a cost-effective manner while maintaining the quality of products manufactured. Further research on the economics of all measures?as well as on their applicability to different production practices?is needed to assess their cost effectiveness at individual plants.

  9. Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis.

    PubMed

    Bosch, Dustin E; Kimple, Adam J; Manning, Alyssa J; Muller, Robin E; Willard, Francis S; Machius, Mischa; Rogers, Stephen L; Siderovski, David P

    2013-01-01

    G protein signaling pathways, as key components of physiologic responsiveness and timing, are frequent targets for pharmacologic intervention. Here, we identify an effector for heterotrimeric G protein α subunit (EhGα1) signaling from Entamoeba histolytica, the causative agent of amoebic colitis. EhGα1 interacts with this effector and guanosine triphosphatase-accelerating protein, EhRGS-RhoGEF, in a nucleotide state-selective fashion. Coexpression of EhRGS-RhoGEF with constitutively active EhGα1 and EhRacC leads to Rac-dependent spreading in Drosophila S2 cells. EhRGS-RhoGEF overexpression in E. histolytica trophozoites leads to reduced migration toward serum and lower cysteine protease activity, as well as reduced attachment to, and killing of, host cells. A 2.3 Å crystal structure of the full-length EhRGS-RhoGEF reveals a putative inhibitory helix engaging the Dbl homology domain Rho-binding surface and the pleckstrin homology domain. Mutational analysis of the EhGα1/EhRGS-RhoGEF interface confirms a canonical "regulator of G protein signaling" domain rather than a RhoGEF-RGS ("rgRGS") domain, suggesting a convergent evolution toward heterotrimeric and small G protein cross-talk. PMID:23260656

  10. Structural Determinants of RGS-RhoGEF Signaling Critical to Entamoeba histolytica Pathogenesis

    PubMed Central

    Bosch, Dustin E.; Kimple, Adam J.; Manning, Alyssa J.; Muller, Robin E.; Willard, Francis S.; Machius, Mischa; Rogers, Stephen L.; Siderovski, David P.

    2012-01-01

    Summary G-protein signaling pathways, as key components of physiologic responsiveness and timing, are frequent targets for pharmacologic intervention. Here, we identify an effector for heterotrimeric G-protein α subunit (EhGα1) signaling from Entamoeba histolytica, the causative agent of amoebic colitis. EhGα1 interacts with this effector and GTPase-accelerating protein (GAP), EhRGS-RhoGEF, in a nucleotide state-selective fashion. Co-expression of EhRGS-RhoGEF with constitutively active EhGα1 and EhRacC leads to Rac-dependent spreading in Drosophila S2 cells. EhRGS-RhoGEF overexpression in E. histolytica trophozoites leads to reduced migration toward serum and lower cysteine protease activity, as well as reduced attachment to, and killing of, host cells. A 2.3 Å crystal structure of the full-length EhRGS-RhoGEF reveals a putative inhibitory helix engaging the DH domain Rho-binding surface and the PH domain. Mutational analysis of the EhGα1/EhRGS-RhoGEF interface confirms a canonical RGS domain rather than a RhoGEF-RGS (“rgRGS”) domain, suggesting a convergent evolution toward heterotrimeric and small G-protein cross-talk. PMID:23260656

  11. Absence of mutations in major GEFS+ genes in myoclonic astatic epilepsy.

    PubMed

    Nabbout, R; Kozlovski, A; Gennaro, E; Bahi-Buisson, N; Zara, F; Chiron, C; Bianchi, A; Brice, A; Leguern, E; Dulac, O

    2003-10-01

    Myoclonic astatic epilepsy (MAE) is a genetically determined condition of childhood onset characterized by multiple generalized types of seizures including myoclonic astatic seizures, generalized spike waves and cognitive deterioration. This condition has been reported in a few patients in generalized epilepsy with febrile seizures plus (GEFS+) families and MAE has been considered, like severe myoclonic epilepsy of infancy (SMEI), to be a severe phenotype within the GEFS+ spectrum. Four genes have been identified in GEFS+ families, but only three (SCN1A, SCNlB, GABRG2) were found in MAE patients within GEFS+ families. We analysed these three genes in a series of 22 sporadic patients with MAE and found no causal mutations. These findings suggest that MAE, unlike SMEI, is not genetically related to GEFS+. Although MAE and SMEI share the same types of seizures, only SMEI patients are sensitive to fever. This is probably its main link to GEFS+. A different family of genes is likely to account for MAE. PMID:14642997

  12. A pull-down procedure for the identification of unknown GEFs for small GTPases

    PubMed Central

    Koch, Daniel; Rai, Amrita; Ali, Imtiaz; Bleimling, Nathalie; Friese, Timon; Brockmeyer, Andreas; Janning, Petra; Goud, Bruno; Itzen, Aymelt; Müller, Matthias P.; Goody, Roger S.

    2016-01-01

    ABSTRACT Members of the family of small GTPases regulate a variety of important cellular functions. In order to accomplish this, tight temporal and spatial regulation is absolutely necessary. The two most important factors for this regulation are GTPase activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs), the latter being responsible for the activation of the GTPase downstream pathways at the correct location and time. Although a large number of exchange factors have been identified, it is likely that a similarly large number remains unidentified. We have therefore developed a procedure to specifically enrich GEF proteins from biological samples making use of the high affinity binding of GEFs to nucleotide-free GTPases. In order to verify the results of these pull-down experiments, we have additionally developed two simple validation procedures: An in vitro transcription/translation system coupled with a GEF activity assay and a yeast two-hybrid screen for detection of GEFs. Although the procedures were established and tested using the Rab protein Sec4, the similar basic principle of action of all nucleotide exchange factors will allow the method to be used for identification of unknown GEFs of small GTPases in general. PMID:26918858

  13. G Protein Activation without a GEF in the Plant Kingdom

    PubMed Central

    Wang, Hao; Matthews, Melissa; Bradford, William; Bennetzen, Jeffrey L.; Jones, Alan M.

    2012-01-01

    Animal heterotrimeric G proteins are activated by guanine nucleotide exchange factors (GEF), typically seven transmembrane receptors that trigger GDP release and subsequent GTP binding. In contrast, the Arabidopsis thaliana G protein (AtGPA1) rapidly activates itself without a GEF and is instead regulated by a seven transmembrane Regulator of G protein Signaling (7TM-RGS) protein that promotes GTP hydrolysis to reset the inactive (GDP-bound) state. It is not known if this unusual activation is a major and constraining part of the evolutionary history of G signaling in eukaryotes. In particular, it is not known if this is an ancestral form or if this mechanism is maintained, and therefore constrained, within the plant kingdom. To determine if this mode of signal regulation is conserved throughout the plant kingdom, we analyzed available plant genomes for G protein signaling components, and we purified individually the plant components encoded in an informative set of plant genomes in order to determine their activation properties in vitro. While the subunits of the heterotrimeric G protein complex are encoded in vascular plant genomes, the 7TM-RGS genes were lost in all investigated grasses. Despite the absence of a Gα-inactivating protein in grasses, all vascular plant Gα proteins examined rapidly released GDP without a receptor and slowly hydrolyzed GTP, indicating that these Gα are self-activating. We showed further that a single amino acid substitution found naturally in grass Gα proteins reduced the Gα-RGS interaction, and this amino acid substitution occurred before the loss of the RGS gene in the grass lineage. Like grasses, non-vascular plants also appear to lack RGS proteins. However, unlike grasses, one representative non-vascular plant Gα showed rapid GTP hydrolysis, likely compensating for the loss of the RGS gene. Our findings, the loss of a regulatory gene and the retention of the “self-activating” trait, indicate the existence of divergent

  14. Economic efficiency in fish farming: hope for agro-allied industries in Niagara

    NASA Astrophysics Data System (ADS)

    Kareem, R. O.; Dipeolu, A. O.; Aromolaran, A. B.; Williams, S. B.

    2008-02-01

    The challenge to increase the efficiency in food production level in Nigeria appears to be more urgent now than it has ever been in the history of the country. This is in view of the rapidly increasing population, the imminent decline in international economic and food aid and the need to conserve foreign exchange earnings through the production of raw materials to feed the growing industrial sector calls for urgent attention. The study was carried out in Ogun State. The descriptive statistics was used to determine the socio-economic characteristics of the respondents. The stochastic frontiers production analysis was applied to estimate the technical, allocative efficiency and economic efficiency among the fish farmers in the state. The results of economic efficiency revealed that fish farming is economically efficient with a range of between 55% and 84% efficiency level suggesting a favourable hope for the agro-allied industry such as poultry and cottage industries etc in the state. The result of hypothesis of inefficiency sources models showed that years of experience of fish farmers is significant at 1% probability level indicating the factor contributing to the fish farming experience in the state. Thus, the efficiency is due to the fact that farmers are experienced and fairly educated. On the basis of findings, policy is suggested to be directed towards the encouragement of entrepreneurs in fish farming in the state by providing enabling environment like credit facilities, public enlightenment programme and provision of social amenities like feeder roads, pipe-born water etc and given the fact that an increase in the level of formal education variable leads to less inefficiency, government policy should be focused on adopting the best technology (e.g. fast growing species and equipment) so as to improve the level of efficiency and investment which shall eventually lead to growth in output of fish farming and a lead to the establishment of agro

  15. Strategies for reconciling environmental goals, productivity improvement, and increased energy efficiency in the industrial sector: Analytic framework

    SciTech Connect

    Boyd, G.A.

    1995-06-01

    The project is motivated by recommendations that were made by industry in a number of different forums: the Industry Workshop of the White House Conference on Climate Change, and more recently, industry consultations for EPAct Section 131(c) and Section 160(b). These recommendations were related to reconciling conflicts in environmental goals, productivity improvements and increased energy efficiency in the industrial sector.

  16. Assessing Energy Efficiency Opportunities in US Industrial and Commercial Building Motor Systems

    SciTech Connect

    Rao, Prakash; Sheaffer, Paul; McKane, Aimee; Scheihing, Paul

    2015-09-01

    In 2002, the United States Department of Energy (USDOE) published an energy efficiency assessment of U.S. industrial sector motor systems titled United States Industrial Electric Motor Systems Market Opportunities Assessment. The assessment advanced motor system efficiency by providing a greater understanding of the energy consumption, use characteristics, and energy efficiency improvement potential of industrial sector motor systems in the U.S. Since 2002, regulations such as Minimum Energy Performance Standards, cost reductions for motor system components such as variable frequency drives, system-integrated motor-driven equipment, and awareness programs for motor system energy efficiency have changed the landscape of U.S. motor system energy consumption. To capture the new landscape, the USDOE has initiated a three-year Motor System Market Assessment (MSMA), led by Lawrence Berkeley National Laboratory (LBNL). The MSMA will assess the energy consumption, operational and maintenance characteristics, and efficiency improvement opportunity of U.S. industrial sector and commercial building motor systems. As part of the MSMA, a significant effort is currently underway to conduct field assessments of motor systems from a sample of facilities representative of U.S. commercial and industrial motor system energy consumption. The Field Assessment Plan used for these assessments builds on recent LBNL research presented at EEMODS 2011 and EEMODS 2013 using methods for characterizing and determining regional motor system energy efficiency opportunities. This paper provides an update on the development and progress of the MSMA, focusing on the Field Assessment Plan and the framework for assessing the global supply chain for emerging motors and drive technologies.

  17. The RalGEF-Ral Effector Signaling Network

    PubMed Central

    Neel, Nicole F.; Martin, Timothy D.; Stratford, Jeran K.; Zand, Tanya P.; Reiner, David J.; Der, Channing J.

    2011-01-01

    The high frequency of RAS mutations in human cancers (33%) has stimulated intense interest in the development of anti-Ras inhibitors for cancer therapy. Currently, the major focus of these efforts is centered on inhibitors of components involved in Ras downstream effector signaling. In particular, more than 40 inhibitors of the Raf-MEK-ERK mitogen-activated protein kinase cascade and phosphoinositide 3-kinase-AKT-mTOR effector signaling networks are currently under clinical evaluation. However, these efforts are complicated by the fact that Ras can utilize at least 9 additional functionally distinct effectors, with at least 3 additional effectors with validated roles in Ras-mediated oncogenesis. Of these, the guanine nucleotide exchange factors of the Ras-like (Ral) small GTPases (RalGEFs) have emerged as important effectors of mutant Ras in pancreatic, colon, and other cancers. In this review, we summarize the evidence for the importance of this effector pathway in cancer and discuss possible directions for therapeutic inhibition of aberrant Ral activation and signaling. PMID:21779498

  18. Energy Efficiency Improvement and Cost Saving Oportunities for the Concrete Industry

    SciTech Connect

    Kermeli, Katerina; Worrell, Ernst; Masanet, Eric

    2011-12-01

    The U.S. concrete industry is the main consumer of U.S.-produced cement. The manufacturing of ready mixed concrete accounts for more than 75% of the U.S. concrete production following the manufacturing of precast concrete and masonry units. The most significant expenditure is the cost of materials accounting for more than 50% of total concrete production costs - cement only accounts for nearly 24%. In 2009, energy costs of the U.S. concrete industry were over $610 million. Hence, energy efficiency improvements along with efficient use of materials without negatively affecting product quality and yield, especially in times of increased fuel and material costs, can significantly reduce production costs and increase competitiveness. The Energy Guide starts with an overview of the U.S. concrete industry’s structure and energy use, a description of the various manufacturing processes, and identification of the major energy consuming areas in the different industry segments. This is followed by a description of general and process related energy- and cost-efficiency measures applicable to the concrete industry. Specific energy and cost savings and a typical payback period are included based on literature and case studies, when available. The Energy Guide intends to provide information on cost reduction opportunities to energy and plant managers in the U.S. concrete industry. Every cost saving opportunity should be assessed carefully prior to implementation in individual plants, as the economics and the potential energy and material savings may differ.

  19. Development of a performance-based industrial energy efficiency indicator for cement manufacturing plants.

    SciTech Connect

    Boyd, G.; Decision and Information Sciences

    2006-07-21

    Organizations that implement strategic energy management programs have the potential to achieve sustained energy savings if the programs are carried out properly. A key opportunity for achieving energy savings that plant managers can take is to determine an appropriate level of energy performance by comparing the plant performance with that of similar plants in the same industry. Manufacturing plants can set energy efficiency targets by using performance-based indicators. The U.S. Environmental Protection Agency (EPA), through its ENERGY STAR{reg_sign} program, has been developing plant energy performance indicators (EPIs) to encourage a variety of U.S. industries to use energy more efficiently. This report describes work with the cement manufacturing industry to provide a plant-level indicator of energy efficiency for assembly plants that produce a variety of products, including Portland cement and other specialty cement products, in the United States. Consideration is given to the role that performance-based indicators play in motivating change; the steps needed to develop indicators, including interacting with an industry to secure adequate data for an indicator; and the actual application and use of an indicator when complete. How indicators are employed in the EPA's efforts to encourage industries to voluntarily improve their use of energy is discussed as well. The report describes the data and statistical methods used to construct the EPI for cement manufacturing plants. Individual equations are presented, as are the instructions for using them in an associated Excel spreadsheet.

  20. RopGEF2 is involved in ABA-suppression of seed germination and post-germination growth of Arabidopsis.

    PubMed

    Zhao, Shujuan; Wu, Yuxuan; He, Yuqing; Wang, Yarui; Xiao, Jun; Li, Lin; Wang, Yanping; Chen, Xi; Xiong, Wei; Wu, Yan

    2015-12-01

    The involvement of Rho of Plants (ROP) GTPases in abscisic acid (ABA) signalling in Arabidopsis has been demonstrated in many studies. However, the roles of RopGEFs (Rop guanine nucleotide exchange factors), which modulate ROP activities in ABA signalling, are poorly understood. Here, we demonstrate that RopGEF2 may play a negative role in ABA-suppressed seed germination and post-germination growth. We show that disruption of RopGEF2 enhances sensitivity to exogenous ABA in seed germination assays and that RopGEF2pro-GUS is mainly expressed in developing embryos and germinating seeds. Interestingly, YFP-RopGEF2 is located in both the cytoplasmic region and in mitochondria. Notably, the PRONE2 (plant-specific ROP nucleotide exchanger 2) domain of RopGEF2 is detected in mitochondria, whereas the N-terminus of RopGEF2 is shown to be in the cytosol. After ABA treatment, degradation of RopGEF2 is triggered in the cytosol through the ubiquitin-26S proteasome system. The binding of RopGEF2 to ROP2, ROP6 or ROP10, which has been demonstrated to be involved in ABA signalling, not only alters the localization of RopGEF2 but also enables RopGEF2 to escape degradation in the cell. Thus, in this study, we deduce a sophisticated mechanism of ABA-mediated RopGEF2-ROP signalling, which potentially implicates the inactivation of ROPs in responsiveness to ABA. PMID:26461226

  1. The Failure of Eco-Efficiency to Guarantee Sustainability: Future Challenges for Industrial Ecology

    SciTech Connect

    Huesemann, Michael H.

    2004-12-01

    Western industrialized societies are inherently unsustainable in their present form because they depend almost exclusively on a finite supply of non-renewable minerals and fossil fuels. In addition, the resulting wastes cause various environmental problems ranging from widespread ecosystem disruptions to global warming. The most common response to these problems has been to promote technological improvements in eco-efficiency, which may be defined as ''adding maximum value with minimum resource use and minimum pollution'' (Welford 1997). While constructive, improvements in eco-efficiency alone will not guarantee sustainability of industrialized societies because the limited supplies of non-renewable minerals cannot be extended indefinitely via recycling and substitution, and a transition to renewable and nuclear energy sources would result in significant negative environmental impacts, particularly if deployed on a large scale. In addition, according to the second law of thermodynamics, industrial production technologies have inherently unavoidable environmental impacts. Finally, any hard won improvements in eco-efficiency will soon be negated if growth in population and consumption is allowed to continue. Consequently, long-term industrial sustainability can only be achieved through a transition to a steady-state economy where the total throughput of matter-energy is kept at a constant and sustainable level. This requires not only improvements in eco-efficiency but also a reassessment of fundamental societal values that erroneously equate material consumption and economic growth with well-being and happiness.

  2. ETV REPORT: EVALUATION OF HYDROMETRICS, INC., HIGH EFFICIENCY REVERSE OSMOSIS (HERO™) INDUSTRIAL WASTEWATER TREATMENT SYSTEM

    EPA Science Inventory

    Hydrometrics, founded in 1979 and located in Helena, MT, manufactures a commercial-ready High Efficiency Reverse Osmosis (HERO™) industrial wastewater treatment system. The system uses a three-stage reverse osmosis process to remove and concentrate metals for recovery while prod...

  3. 78 FR 12251 - Energy Efficiency Program for Commercial and Industrial Equipment: Public Meeting and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-22

    ... covered equipment under the Energy Policy and Conservation Act of 1975, as amended (76 FR 37628, June 28... document to consider such standards (78 FR 7306, Feb. 1, 2013). The framework document requested public... Part 430 RIN 1904-AC55 Energy Efficiency Program for Commercial and Industrial Equipment:...

  4. Competition policy and the transition to a low-carbon, efficient electricity industry

    SciTech Connect

    Moss, Diana L.; Kwoka, John E. Jr.

    2010-08-15

    U.S. industries are facing intense pressures to become more energy efficient. Two concerns are driving this transition. One is the need to lower the carbon footprints of energy-intensive sectors. A second concern is the need to achieve energy security by reducing this country's reliance on foreign sources of energy supplies. (author)

  5. EVALUATION OF THE EFFICIENCY OF INDUSTRIAL FLARES: FLARE HEAD DESIGN AND GAS COMPOSITION

    EPA Science Inventory

    The report gives continued Phase 4 results of a research program to quantify emissions from, and efficiencies of, industrial flares. Initial results were limited to tests conducted burning propane/nitrogen mixtures in pipe flares without pilot light stabilization. The work report...

  6. EVALUATION OF COMBUSTION MODIFICATION EFFECTS ON EMISSIONS AND EFFICIENCY OF WOOD-FIRED INDUSTRIAL BOILERS

    EPA Science Inventory

    The report gives results of full-scale tests to evaluate combustion modifications (lower excess air and variations in the overfire air system operation) for emission control and efficiency enhancement on two wood-fired industrial boilers. Polycyclic organic matter (POM) was sampl...

  7. Advanced Thermoelectric Materials for Efficient Waste Heat Recovery in Process Industries

    SciTech Connect

    Adam Polcyn; Moe Khaleel

    2009-01-06

    The overall objective of the project was to integrate advanced thermoelectric materials into a power generation device that could convert waste heat from an industrial process to electricity with an efficiency approaching 20%. Advanced thermoelectric materials were developed with figure-of-merit ZT of 1.5 at 275 degrees C. These materials were not successfully integrated into a power generation device. However, waste heat recovery was demonstrated from an industrial process (the combustion exhaust gas stream of an oxyfuel-fired flat glass melting furnace) using a commercially available (5% efficiency) thermoelectric generator coupled to a heat pipe. It was concluded that significant improvements both in thermoelectric material figure-of-merit and in cost-effective methods for capturing heat would be required to make thermoelectric waste heat recovery viable for widespread industrial application.

  8. Certifying Industrial Energy Efficiency Performance: AligningManagement, Measurement, and Practice to Create Market Value

    SciTech Connect

    McKane, Aimee; Scheihing, Paul; Williams, Robert

    2007-07-01

    More than fifteen years after the launch of programs in theU.K. and U.S., industry still offers one of the largest opportunities forenergy savings worldwide. The International Energy Agency (IEA) estimatesthe savings potential from cost-optimization of industrial motor-drivensystems alone at 7 percent of global electricity use. The U.S. Departmentof Energy (USDOE) Industrial Technologies Program estimates 7 percentsavings potential in total US industrial energy use through theapplication of proven best practice. Simple paybacks for these types ofprojects are frequently two years or less. The technology required toachieve these savings is widely available; the technical skills requiredto identify energy saving opportunities are known and transferable.Although programs like USDOE's Best Practices have been highlysuccessful, most plants, as supported by 2002 MECS data, remain eitherunaware or unmotivated to improve their energy efficiency--as evidencedby the 98 percent of US industrial facilities reporting to MECS say thatthey lack a full-time energy manager. With the renewed interest in energyefficiency worldwide and the emergence of carbon trading and newfinancial instruments such as white certificates1, there is a need tointroduce greater transparency into the way that industrial facilitiesidentify, develop, and document energy efficiency projects. Historically,industrial energy efficiency projects have been developed by plantengineers, frequently with assistance from consultants and/or supplierswith highly specialized technical skills. Under this scenario,implementation of energy efficiency improvements is dependent onindividuals. These individuals typically include "champions" within anindustrial facility or corporation, working in cooperation withconsultants or suppliers who have substantial knowledge based on years ofexperience. This approach is not easily understood by others without thisspecialized technical knowledge, penetrates the market fairly slowly

  9. Thinking Globally: How ISO 50001 - Energy Management can make industrial energy efficiency standard practice

    SciTech Connect

    McKane, Aimee; Desai, Deann; Matteini, Marco; Meffert, William; Williams, Robert; Risser, Roland

    2009-08-01

    Industry utilizes very complex systems, consisting of equipment and their human interface, which are organized to meet the production needs of the business. Effective and sustainable energy efficiency programs in an industrial setting require a systems approach to optimize the integrated whole while meeting primary business requirements. Companies that treat energy as a manageable resource and integrate their energy program into their management practices have an organizational context to continually seek opportunities for optimizing their energy use. The purpose of an energy management system standard is to provide guidance for industrial and commercial facilities to integrate energy efficiency into their management practices, including fine-tuning production processes and improving the energy efficiency of industrial systems. The International Organization for Standardization (ISO) has identified energy management as one of its top five priorities for standards development. The new ISO 50001 will establish an international framework for industrial, commercial, or institutional facilities, or entire companies, to manage their energy, including procurement and use. This standard is expected to achieve major, long-term increases in energy efficiency (20percent or more) in industrial, commercial, and institutional facilities and to reduce greenhouse gas (GHG) emissions worldwide.This paper describes the impetus for the international standard, its purpose, scope and significance, and development progress to date. A comparative overview of existing energy management standards is provided, as well as a discussion of capacity-building needs for skilled individuals to assist organizations in adopting the standard. Finally, opportunities and challenges are presented for implementing ISO 50001 in emerging economies and developing countries.

  10. Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis.

    PubMed

    Wei, Bin; Hercyk, Brian S; Mattson, Nicholas; Mohammadi, Ahmad; Rich, Julie; DeBruyne, Erica; Clark, Mikayla M; Das, Maitreyi

    2016-04-15

    The Rho-family GTPase Cdc42 regulates cell polarity and localizes to the cell division site. Cdc42 is activated by guanine nucleotide exchange factors (GEFs). We report that Cdc42 promotes cytokinesis via a unique spatiotemporal activation pattern due to the distinct action of its GEFs, Gef1 and Scd1, in fission yeast. Before cytokinetic ring constriction, Cdc42 activation, is Gef1 dependent, and after ring constriction, it is Scd1 dependent. Gef1 localizes to the actomyosin ring immediately after ring assembly and promotes timely onset of ring constriction. Gef1 is required for proper actin organization during cytokinesis, distribution of type V myosin Myo52 to the division site, and timely recruitment of septum protein Bgs1. In contrast, Scd1 localizes to the broader region of ingressing membrane during cytokinetic furrowing. Scd1 promotes normal septum formation, andscd1Δcells display aberrant septa with reduced Bgs1 localization. Thus we define unique roles of the GEFs Gef1 and Scd1 in the regulation of distinct events during cytokinesis. Gef1 localizes first to the cytokinetic ring and promotes timely constriction, whereas Scd1 localizes later to the ingressing membrane and promotes septum formation. Our findings are consistent with reports that complexity in GTPase signaling patterns enables exquisite precision over the control of cellular processes. PMID:26941334

  11. Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis

    PubMed Central

    Wei, Bin; Hercyk, Brian S.; Mattson, Nicholas; Mohammadi, Ahmad; Rich, Julie; DeBruyne, Erica; Clark, Mikayla M.; Das, Maitreyi

    2016-01-01

    The Rho-family GTPase Cdc42 regulates cell polarity and localizes to the cell division site. Cdc42 is activated by guanine nucleotide exchange factors (GEFs). We report that Cdc42 promotes cytokinesis via a unique spatiotemporal activation pattern due to the distinct action of its GEFs, Gef1 and Scd1, in fission yeast. Before cytokinetic ring constriction, Cdc42 activation, is Gef1 dependent, and after ring constriction, it is Scd1 dependent. Gef1 localizes to the actomyosin ring immediately after ring assembly and promotes timely onset of ring constriction. Gef1 is required for proper actin organization during cytokinesis, distribution of type V myosin Myo52 to the division site, and timely recruitment of septum protein Bgs1. In contrast, Scd1 localizes to the broader region of ingressing membrane during cytokinetic furrowing. Scd1 promotes normal septum formation, and scd1Δ cells display aberrant septa with reduced Bgs1 localization. Thus we define unique roles of the GEFs Gef1 and Scd1 in the regulation of distinct events during cytokinesis. Gef1 localizes first to the cytokinetic ring and promotes timely constriction, whereas Scd1 localizes later to the ingressing membrane and promotes septum formation. Our findings are consistent with reports that complexity in GTPase signaling patterns enables exquisite precision over the control of cellular processes. PMID:26941334

  12. National Residential Efficiency Measures Database Aimed at Reducing Risk for Residential Retrofit Industry (Fact Sheet)

    SciTech Connect

    Not Available

    2012-01-01

    This technical highlight describes NREL research to develop a publicly available database of energy retrofit measures containing performance characteristics and cost estimates for nearly 3,000 measures. Researchers at the U.S. Department of Energy (DOE) National Renewable Energy Laboratory (NREL) have developed the National Residential Efficiency Measures Database, a public database that characterizes the performance and costs of common residential energy efficiency measures. The data are available for use in software programs that evaluate cost-effective retrofit measures to improve the energy efficiency of residential buildings. The database provides a single, consistent source of current data for DOE and private-sector energy audit and simulation software tools and the retrofit industry. The database will reduce risk for residential retrofit industry stakeholders by providing a central, publicly vetted source of up-to-date information.

  13. Industry efficiency and total factor productivity growth under resources and environmental constraint in China.

    PubMed

    Tao, Feng; Li, Ling; Xia, X H

    2012-01-01

    The growth of China's industry has been seriously depending on energy and environment. This paper attempts to apply the directional distance function and the Luenberger productivity index to measure the environmental efficiency, environmental total factor productivity, and its components at the level of subindustry in China over the period from 1999 to 2009 while considering energy consumption and emission of pollutants. This paper also empirically examines the determinants of efficiency and productivity change. The major findings are as follows. Firstly, the main sources of environmental inefficiency of China's industry are the inefficiency of gross industrial output value, the excessive energy consumption, and pollutant emissions. Secondly, the highest growth rate of environmental total factor productivity among the three industrial categories is manufacturing, followed by mining, and production and supply of electricity, gas, and water. Thirdly, foreign direct investment, capital-labor ratio, ownership structure, energy consumption structure, and environmental regulation have varying degrees of effects on the environmental efficiency and environmental total factor productivity. PMID:23365517

  14. Evaluation on the efficiency of biomass power generation industry in china.

    PubMed

    Sun, Jingqi; Sun, Dong; Guo, Sen

    2014-01-01

    As a developing country with large population, China is facing the problems of energy resource shortage and growing environmental pollution arising from the coal-dominated energy structure. Biomass energy, as a kind of renewable energy with the characteristics of being easy to store and friendly to environment, has become the focus of China's energy development in the future. Affected by the advanced power generation technology and diversified geography environment, the biomass power generation projects show new features in recent years. Hence, it is necessary to evaluate the efficiency of biomass power generation industry by employing proper method with the consideration of new features. In this paper, the regional difference as a new feature of biomass power generation industry is taken into consideration, and the AR model is employed to modify the zero-weight issue when using data envelopment analysis (DEA) method to evaluate the efficiency of biomass power generation industry. 30 biomass power generation enterprises in China are selected as the sample, and the efficiency evaluation is performed. The result can provide some insights into the sustainable development of biomass power generation industry in China. PMID:25093209

  15. Industry Efficiency and Total Factor Productivity Growth under Resources and Environmental Constraint in China

    PubMed Central

    Tao, Feng; Li, Ling; Xia, X. H.

    2012-01-01

    The growth of China's industry has been seriously depending on energy and environment. This paper attempts to apply the directional distance function and the Luenberger productivity index to measure the environmental efficiency, environmental total factor productivity, and its components at the level of subindustry in China over the period from 1999 to 2009 while considering energy consumption and emission of pollutants. This paper also empirically examines the determinants of efficiency and productivity change. The major findings are as follows. Firstly, the main sources of environmental inefficiency of China's industry are the inefficiency of gross industrial output value, the excessive energy consumption, and pollutant emissions. Secondly, the highest growth rate of environmental total factor productivity among the three industrial categories is manufacturing, followed by mining, and production and supply of electricity, gas, and water. Thirdly, foreign direct investment, capital-labor ratio, ownership structure, energy consumption structure, and environmental regulation have varying degrees of effects on the environmental efficiency and environmental total factor productivity. PMID:23365517

  16. Evaluation on the Efficiency of Biomass Power Generation Industry in China

    PubMed Central

    Sun, Dong; Guo, Sen

    2014-01-01

    As a developing country with large population, China is facing the problems of energy resource shortage and growing environmental pollution arising from the coal-dominated energy structure. Biomass energy, as a kind of renewable energy with the characteristics of being easy to store and friendly to environment, has become the focus of China's energy development in the future. Affected by the advanced power generation technology and diversified geography environment, the biomass power generation projects show new features in recent years. Hence, it is necessary to evaluate the efficiency of biomass power generation industry by employing proper method with the consideration of new features. In this paper, the regional difference as a new feature of biomass power generation industry is taken into consideration, and the AR model is employed to modify the zero-weight issue when using data envelopment analysis (DEA) method to evaluate the efficiency of biomass power generation industry. 30 biomass power generation enterprises in China are selected as the sample, and the efficiency evaluation is performed. The result can provide some insights into the sustainable development of biomass power generation industry in China. PMID:25093209

  17. Development of a performance-based industrial energy efficiency indicator for corn refining plants.

    SciTech Connect

    Boyd, G. A.; Decision and Information Sciences; USEPA

    2006-07-31

    Organizations that implement strategic energy management programs have the potential to achieve sustained energy savings if the programs are carried out properly. A key opportunity for achieving energy savings that plant managers can take is to determine an appropriate level of energy performance by comparing their plant's performance with that of similar plants in the same industry. Manufacturing facilities can set energy efficiency targets by using performance-based indicators. The U.S. Environmental Protection Agency (EPA), through its ENERGY STAR{reg_sign} program, has been developing plant energy performance indicators (EPIs) to encourage a variety of U.S. industries to use energy more efficiently. This report describes work with the corn refining industry to provide a plant-level indicator of energy efficiency for facilities that produce a variety of products--including corn starch, corn oil, animal feed, corn sweeteners, and ethanol--for the paper, food, beverage, and other industries in the United States. Consideration is given to the role that performance-based indicators play in motivating change; the steps needed to develop indicators, including interacting with an industry to secure adequate data for an indicator; and the actual application and use of an indicator when complete. How indicators are employed in the EPA's efforts to encourage industries to voluntarily improve their use of energy is discussed as well. The report describes the data and statistical methods used to construct the EPI for corn refining plants. Individual equations are presented, as are the instructions for using them in an associated Excel spreadsheet.

  18. An examination of competition and efficiency for hospital industry in Turkey.

    PubMed

    Özgen Narcı, Hacer; Ozcan, Yasar A; Şahin, İsmet; Tarcan, Menderes; Narcı, Mustafa

    2015-12-01

    The two particular reforms that have been undertaken under the Health Transformation Program in Turkey are enhancing efficiency and increasing competition. However, there is a lack of information about the relationship between competition and hospital efficiency. The purpose of this paper is to analyze the effect of competition on technical efficiency for the hospital industry in Turkey. The target population included all public and private general hospitals that were open in 2010 in Turkey (n = 1,224). From these, 1,103 hospitals met the selection criteria and were included in the study. Data were obtained from the Turkish Statistical Institute, the Ministry of Health, and through a field survey. Technical efficiency of hospitals was estimated using Data Envelopment Analysis with five outputs and five inputs. The intensity of competition among hospitals was measured by objective and subjective measures. Objective competition was measured using the Hirschman-Herfindahl Index, and subjective competition was measured based on the perceptions of top level hospital managers. Multivariate Tobit regression was used to investigate the relationship between competition and efficiency while controlling the effects of demand and supply characteristics of the market and the hospital traits. Efficiency results showed that 17% of hospitals were technically efficient. Regression analyses portrayed that the degree of competition among general hospitals did not have a statistically significant relationship with hospitals' technical efficiency. To conclude, hospital efficiency in Turkey does not seem to be affected by the intensity of competition among hospitals. PMID:25515038

  19. Energy efficiency and advanced technologies in the iron and steel industry

    SciTech Connect

    Worrell, E.; Moore, C.

    1997-07-01

    The iron and steel industry is one of the largest energy-consuming and energy-intensive industrial sectors in the world. In the US it consumes approximately 8% of total manufacturing energy consumption. The development of energy efficiency and penetration rate of various technologies, e.g., BOF steelmaking and continuous casting is discussed and compared. Between 1980 and 1991 energy intensity of the US steel industry has declined by 17%, of which 11 %-points are due to efficiency improvements. Considerable potential for energy efficiency improvement can be realized by applying currently best available technology (the so-called technical potential). For the US the technical potential is estimated to be 43{+-}8%, taking the current industrial structure (mix of raw materials used and products produced) into account and the best available technology. The economically profitable potential is estimated to be 10--15%-points lower. The potential for energy efficiency improvement in the US is higher than that in other OECD countries, despite the improvements since 1980. Advanced technologies, such as smelt reduction and near net shape casting, present major opportunities for further reduction in energy consumption at potentially lower costs, as well as environmental benefits. This slab casting has first been introduced in the US by mini-mills to compete on the steel sheet markets. Since the first commercial introduction in 1989 the technology has shown rapid development and capacity growth. Smelt reduction on the other hand does not yet show such a rapid development. The authors discuss the status of smelt reduction, as well as the potential opportunities for the US integrated steel industry.

  20. Regulating Rac in the Nervous System: Molecular Function and Disease Implication of Rac GEFs and GAPs

    PubMed Central

    Bai, Yanyang; Xiang, Xiaoliang; Liang, Chunmei

    2015-01-01

    Rho family GTPases, including RhoA, Rac1, and Cdc42 as the most studied members, are master regulators of actin cytoskeletal organization. Rho GTPases control various aspects of the nervous system and are associated with a number of neuropsychiatric and neurodegenerative diseases. The activity of Rho GTPases is controlled by two families of regulators, guanine nucleotide exchange factors (GEFs) as the activators and GTPase-activating proteins (GAPs) as the inhibitors. Through coordinated regulation by GEFs and GAPs, Rho GTPases act as converging signaling molecules that convey different upstream signals in the nervous system. So far, more than 70 members of either GEFs or GAPs of Rho GTPases have been identified in mammals, but only a small subset of them have well-known functions. Thus, characterization of important GEFs and GAPs in the nervous system is crucial for the understanding of spatiotemporal dynamics of Rho GTPase activity in different neuronal functions. In this review, we summarize the current understanding of GEFs and GAPs for Rac1, with emphasis on the molecular function and disease implication of these regulators in the nervous system. PMID:25879033

  1. Tax and Fiscal Policies for Promotion of Industrial EnergyEfficiency: A Survey of International Experience

    SciTech Connect

    Price, Lynn; Galitsky, Christina; Sinton, Jonathan; Worrell,Ernst; Graus, Wina

    2005-09-15

    The Energy Foundation's China Sustainable Energy Program (CSEP) has undertaken a major project investigating fiscal and tax policy options for stimulating energy efficiency and renewable energy development in China. This report, which is part of the sectoral sub-project studies on energy efficiency in industry, surveys international experience with tax and fiscal policies directed toward increasing investments in energy efficiency in the industrial sector. The report begins with an overview of tax and fiscal policies, including descriptions and evaluations of programs that use energy or energy-related carbon dioxide (CO2) taxes, pollution levies, public benefit charges, grants or subsidies, subsidized audits, loans, tax relief for specific technologies, and tax relief as part of an energy or greenhouse gas (GHG) emission tax or agreement scheme. Following the discussion of these individual policies, the report reviews experience with integrated programs found in two countries as well as with GHG emissions trading programs. The report concludes with a discussion of the best practices related to international experience with tax and fiscal policies to encourage investment in energy efficiency in industry.

  2. Improving the resource efficiency of the German steel industry using material flow analysis

    NASA Astrophysics Data System (ADS)

    Müller, J. I. R.; Mayer, W. A.

    2015-03-01

    This article deals with the utilization of residues from the German steel industry with focus on electric arc furnace (EAF) dust. Material flow analysis is used to illustrate the steel industry by specific output flows of residues over the process chain. The objective of the underlying study was to find further, more efficient connections between process chains. In Germany EAF dust is mainly processed in the Waelz process to recover the contained zinc (open-loop recycling). About 67 % of the world's EAF dust, however, is landfilled and thereby an amount corresponding to 1 to 10 % of the annual production of zinc is lost for production processes. In this article treatment operations for EAF dust are compared through utility analysis with regard to resource efficiency.

  3. GUIDELINES FOR INDUSTRIAL BOILER PERFORMANCE IMPROVEMENT. (BOILER ADJUSTMENT PROCEDURES TO MINIMIZE AIR POLLUTION AND TO ACHIEVE EFFICIENT USE OF FUEL)

    EPA Science Inventory

    Recommended procedures for improving industrial boiler performance to minimize air pollution and to achieve efficient use of fuel are given. It is intended for use by industrial boiler operators to perform an efficiency and emissions tune-up on boilers firing gas, oil, or coal. P...

  4. Efficiency measurement for regulatory market-structure regimes of the electric power industry

    NASA Astrophysics Data System (ADS)

    Briceno, Cecilia Maria

    This dissertation provides an empirical evaluation of market structure reforms that have been implemented in the Electric Power Industry between 1980 and 1999. The analysis is based on the experience of Latin American countries and to some extent of OECD countries. Market structure reforms pose certain tradeoffs. Economic theory suggests that such tradeoffs depend on parameters that can only be estimated econometrically. The tradeoffs arise in industries where vertical relationships between production layers result from the interaction between competitive markets and regulated markets. The implications of different structural arrangements can be clarified by empirical work. This comparative analysis of the recent international experience in the electric power industry addresses these implications. The results of this research support the claim that vertical separation between generation, transmission and distribution is the most efficient regulatory regime, and, as such, a central feature of reform processes in the electric power industry. The empirical analysis uses the distance function as an analytical tool to estimate relative efficiency for each market structure arrangement. For this purpose, the present study assembles a panel set covering 40 countries during a 20-year period.

  5. Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis.

    PubMed

    Okada, Keisuke; Miyake, Hideaki; Yamaguchi, Kohei; Chiba, Koji; Maeta, Kazuhiro; Bilasy, Shymaa E; Edamatsu, Hironori; Kataoka, Tohru; Fujisawa, Masato

    2014-02-28

    Small GTPase Rap1 has been implicated in the proper differentiation of testicular germ cells. In the present study, we investigated the functional significance of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in testicular differentiation using mice lacking RA-GEF-2. RA-GEF-2 was expressed predominantly on the luminal side of the seminiferous tubules in wild-type mice. No significant differences were observed in the body weights or hormonal parameters of RA-GEF-2(-)(/)(-) and wild-type mice. However, the testes of RA-GEF-2(-)(/)(-) male mice were significantly smaller than those of wild-type mice and were markedly atrophied as well as hypospermatogenic. The concentration and motility of epididymal sperm were also markedly reduced and frequently had an abnormal shape. The pregnancy rate and number of fetuses were markedly lower in wild-type females after they mated with RA-GEF-2(-)(/)(-) males than with wild-type males, which demonstrated the male infertility phenotype of RA-GEF-2(-)(/)(-) mice. Furthermore, a significant reduction and alteration were observed in the expression level and cell junctional localization of N-cadherin, respectively, in RA-GEF-2(-)(/)(-) testes, which may, at least in part, account for the defects in testicular differentiation and spermatogenesis in these mice. PMID:24491570

  6. Ultra-Efficient and Power Dense Electric Motors for U. S. Industry

    SciTech Connect

    Melfi, Michael J.; Schiferl, Richard F.; Umans, Stephen D.

    2013-03-12

    The primary purpose of this project was to combine the ease-of-installation and ease-of-use attributes of industrial induction motors with the low-loss and small size and weight advantages of PM motors to create an ultra-efficient, high power density industrial motor that can be started across-the-line or operated from a standard, Volts/Hertz drive without the need for a rotor position feedback device. PM motor products that are currently available are largely variable speed motors that require a special adjustable speed drive with rotor position feedback. The reduced size and weight helps to offset the magnet cost in order make these motors commercially viable. The scope of this project covers horsepower ratings from 20 ? 500. Prototypes were built and tested at ratings ranging from 30 to 250 HP. Since fans, pumps and compressors make up a large portion of industrial motor applications, the motor characteristics are tailored to those applications. Also, since there is extensive use of adjustable frequency inverters in these applications, there is the opportunity to design for an optimal pole number and operate at other than 60 Hz frequency when inverters are utilized. Designs with four and eight pole configurations were prototyped as part of this work. Four pole motors are the most commonly used configuration in induction motors today. The results of the prototype design, fabrication, and testing were quite successful. The 50 HP rating met all of the design goals including efficiency and power density. Tested values of motor losses at 50 HP were 30% lower than energy efficient induction motors and the motor weight is 35% lower than the energy efficient induction motor of the same rating. Further, when tested at the 30 HP rating that is normally built in this 286T frame size, the efficiency far exceeds the project design goals with 30 HP efficiency levels indicating a 55% reduction in loss compared to energy efficient motors with a motor weight that is a few

  7. Clinical and genetic analysis of a new multigenerational pedigree with GEFS+ (Generalized Epilepsy with Febrile Seizures Plus).

    PubMed

    Gérard, Frédérique; Pereira, Sandrine; Robaglia-Schlupp, Andrée; Genton, Pierre; Szepetowski, Pierre

    2002-06-01

    Febrile seizures affect 2-5% of all children younger than 6 years. A small proportion of children with febrile seizures later develop epilepsy. The syndrome of generalized epilepsy with febrile seizures plus (GEFS+) is a heterogeneous disorder characterized by febrile seizures that may persist beyond age 6 years and nonfebrile seizures. Several genes have been localized for FS by linkage analysis, and three GEFS+ genes (SCN1A, SCN1B, GABRG2) have been identified. We identified a large multigenerational family with GEFS+ in France. All affected members had FSs. Among them, seven had other types of epileptic seizures including FSs after age 6 years, nonfebrile generalized seizures, or partial seizures later in life. Genetic linkage study excluded the candidate genes and loci for FS and GEFS+, thus proving the existence of a new GEFS+ genetic locus underlying the phenotype observed in this family. PMID:12060016

  8. Process industry demand for more efficient, more cost-effective heat exchanger tubing

    SciTech Connect

    Thors, P.

    1987-01-01

    In the future the process industry will see a bigger selection of enhanced heat transfer tubes, one of the reasons being the continued production of special patented technology involved in making them. Here the author mentions only some of the factors that might influence the increased usage of these enhanced tubes. In using more efficient tubing in a heat exchanger the designer has available the options to increase the total heat duty per unit volume, lower operating costs by reducing the mean temperature difference at a given heat duty, save material, or reduce the size and/or pumping power, among others. This can be achieved, for example, by replacing plain tubes with appropriate enhanced tubes in retubing applications, where old heat exchangers need to be upgraded and total efficiency improved. When a new heat exchanger is to be built, it is easier for the designer to include the more efficient tubing to utilize all the benefits of the increased thermal performance.

  9. RalB regulates contractility-driven cancer dissemination upon TGFβ stimulation via the RhoGEF GEF-H1

    PubMed Central

    Biondini, Marco; Duclos, Guillaume; Meyer-Schaller, Nathalie; Silberzan, Pascal; Camonis, Jacques; Carla Parrini, Maria

    2015-01-01

    RalA and RalB proteins are key mediators of oncogenic Ras signaling in human oncogenesis. Herein we investigated the mechanistic contribution of Ral proteins to invasion of lung cancer A549 cells after induction of epithelial-mesenchymal transition (EMT) with TGFβ. We show that TGFβ-induced EMT promotes dissemination of A549 cells in a 2/3D assay, independently of proteolysis, by activating the Rho/ROCK pathway which generates actomyosin-dependent contractility forces that actively remodel the extracellular matrix, as assessed by Traction Force microscopy. RalB, but not RalA, is required for matrix deformation and cell dissemination acting via the RhoGEF GEF-H1, which associates with the Exocyst complex, a major Ral effector. Indeed, uncoupling of the Exocyst subunit Sec5 from GEF-H1 impairs RhoA activation, generation of traction forces and cell dissemination. These results provide a novel molecular mechanism underlying the control of cell invasion by RalB via a cross-talk with the Rho pathway. PMID:26152517

  10. Energy efficiency and carbon dioxide emissions reduction opportunities in the U.S. cement industry

    SciTech Connect

    Martin, Nathan; Worrell, Ernst; Price, Lynn

    1999-08-01

    This paper reports on an in-depth analysis of the U.S. cement industry, identifying cost-effective energy efficiency measures and potentials. The authors assess this industry at the aggregate level (Standard Industrial Classification 324), which includes establishments engaged in manufacturing hydraulic cements, including Portland, natural, masonry, and pozzolana when reviewing industry trends and when making international comparisons. Coal and coke are currently the primary fuels for the sector, supplanting the dominance of natural gas in the 1970s. Between 1970 and 1997, primary physical energy intensity for cement production (SIC 324) dropped 30%,from 7.9 GJ/t to 5.6 GJ/t, while carbon dioxide intensity due to fuel consumption (carbon dioxide emissions expressed in tons of carbon per ton cement) dropped 25%, from 0.16 tC/ton to 0.12 tC/ton. Carbon dioxide intensity due to fuel consumption and clinker calcination dropped 17%, from 0.29 tC/ton to 0.24 tC/ton. They examined 30 energy efficient technologies and measures and estimated energy savings, carbon dioxide savings, investment costs, and operation and maintenance costs for each of the measures. They constructed an energy conservation supply curve for U.S. cement industry which found a total cost-effective reduction of 0.6 GJ/ton of cement consisting of measures having a simple payback period of 3 years or less. This is equivalent to potential energy savings of 11% of 1994 energy use for cement making and a savings of 5% of total 1994 carbon dioxide emissions by the U.S. cement industry. Assuming the increased production of blended cement in the U.S., as is common in many parts of the world, the technical potential for energy efficiency improvement would not change considerably. However, the cost-effective potential, would increase to 1.1 GJ/ton cement or 18% of total energy use, and carbon dioxide emissions would be reduced by 16%.

  11. Steam systems in industry: Energy use and energy efficiency improvement potentials

    SciTech Connect

    Einstein, Dan; Worrell, Ernst; Khrushch, Marta

    2001-07-22

    Steam systems are a part of almost every major industrial process today. Thirty-seven percent of the fossil fuel burned in US industry is burned to produce steam. In this paper we will establish baseline energy consumption for steam systems. Based on a detailed analysis of boiler energy use we estimate current energy use in boilers in U.S. industry at 6.1 Quads (6.4 EJ), emitting almost 66 MtC in CO{sub 2} emissions. We will discuss fuels used and boiler size distribution. We also describe potential savings measures, and estimate the economic energy savings potential in U.S. industry (i.e. having payback period of 3 years or less). We estimate the nationwide economic potential, based on the evaluation of 16 individual measures in steam generation and distribution. The analysis excludes the efficient use of steam and increased heat recovery. Based on the analysis we estimate the economic potential at 18-20% of total boiler energy use, resulting in energy savings approximately 1120-1190 TBtu ( 1180-1260 PJ). This results in a reduction of CO{sub 2} emissions equivalent to 12-13 MtC.

  12. Opportunities to improve energy efficiency in the U.S. pulp and paper industry

    SciTech Connect

    Worrell, Ernst; Martin, Nathan; Anglani, Norma; Einstein, Dan; Krushch, Marta; Price, Lynn

    2001-02-02

    This paper analyzes the energy efficiency and carbon dioxide emissions reductions potential of the U.S. pulp and paper industry, one of the largest energy users in the U.S. manufacturing sector. We examined over 45 commercially available state-of-the-art technologies and measures. The measures were characterized, and then ordered on the basis of cost-effectiveness. The report indicates that there still exists significant potential for energy savings and carbon dioxide emissions reduction in this industry. The cost-effective potential for energy efficiency improvement is defined as having a simple pay-back period of three years or less. Not including increased recycling the study identifies a cost-effective savings potential of 16% of the primary energy use in 1994. Including increased recycling leads to a higher potential for energy savings, i.e. a range of cost-effective savings between 16% and 24% of primary energy use. Future work is needed to further elaborate on key energy efficiency measures identified in the report including barriers and opportunities for increased recycling of waste paper.

  13. Analyzing industrial furnace efficiency using comparative visualization in a virtual reality environment.

    SciTech Connect

    Freitag, L.; Urness, T.

    1999-02-10

    We describe an interactive toolkit used to perform comparative analysis of two or more data sets arising from numerical simulations. Several techniques have been incorporated into this toolkit, including (1) successive visualization of individual data sets, (2) data comparison techniques such as computation and visualization of the differences between data sets, and (3) image comparison methods such as scalar field height profiles plotted in a common coordinate system. We describe each technique in detail and show example usage in an industrial application aimed at designing an efficient, low-NOX burner for industrial furnaces. Critical insights are obtained by interactively adjusted color maps, data culling, and data manipulation. New paradigms for scaling small values in the data comparison technique are described. The display device used for this application was the CAVE virtual reality theater, and we describe the user interface to the visualization toolkit and the benefits of immersive 3D visualization for comparative analysis.

  14. Opportunities for Energy Efficiency and Automated Demand Response in Industrial Refrigerated Warehouses in California

    SciTech Connect

    Lekov, Alex; Thompson, Lisa; McKane, Aimee; Rockoff, Alexandra; Piette, Mary Ann

    2009-05-11

    This report summarizes the Lawrence Berkeley National Laboratory's research to date in characterizing energy efficiency and open automated demand response opportunities for industrial refrigerated warehouses in California. The report describes refrigerated warehouses characteristics, energy use and demand, and control systems. It also discusses energy efficiency and open automated demand response opportunities and provides analysis results from three demand response studies. In addition, several energy efficiency, load management, and demand response case studies are provided for refrigerated warehouses. This study shows that refrigerated warehouses can be excellent candidates for open automated demand response and that facilities which have implemented energy efficiency measures and have centralized control systems are well-suited to shift or shed electrical loads in response to financial incentives, utility bill savings, and/or opportunities to enhance reliability of service. Control technologies installed for energy efficiency and load management purposes can often be adapted for open automated demand response (OpenADR) at little additional cost. These improved controls may prepare facilities to be more receptive to OpenADR due to both increased confidence in the opportunities for controlling energy cost/use and access to the real-time data.

  15. Temporal lobe epilepsy and GEFS+ phenotypes associated with SCN1B mutations.

    PubMed

    Scheffer, Ingrid E; Harkin, Louise A; Grinton, Bronwyn E; Dibbens, Leanne M; Turner, Samantha J; Zielinski, Marta A; Xu, Ruwei; Jackson, Graeme; Adams, Judith; Connellan, Mary; Petrou, Steven; Wellard, R Mark; Briellmann, Regula S; Wallace, Robyn H; Mulley, John C; Berkovic, Samuel F

    2007-01-01

    SCN1B, the gene encoding the sodium channel beta 1 subunit, was the first gene identified for generalized epilepsy with febrile seizures plus (GEFS+). Only three families have been published with SCN1B mutations. Here, we present four new families with SCN1B mutations and characterize the associated phenotypes. Analysis of SCN1B was performed on 402 individuals with various epilepsy syndromes. Four probands with missense mutations were identified. Detailed electroclinical phenotyping was performed on all available affected family members including quantitative MR imaging in those with temporal lobe epilepsy (TLE). Two new families with the original C121W SCN1B mutation were identified; novel mutations R85C and R85H were each found in one family. The following phenotypes occurred in the six families with SCN1B missense mutations: 22 febrile seizures, 20 febrile seizures plus, five TLE, three other GEFS+ phenotypes, two unclassified and ten unaffected individuals. All individuals with confirmed TLE had the C121W mutation; two underwent temporal lobectomy (one with hippocampal sclerosis and one without) and both are seizure free. We confirm the role of SCN1B in GEFS+ and show that the GEFS+ spectrum may include TLE alone. TLE with an SCN1B mutation is not a contraindication to epilepsy surgery. PMID:17020904

  16. Digital PCR to assess gene-editing frequencies (GEF-dPCR) mediated by designer nucleases.

    PubMed

    Mock, Ulrike; Hauber, Ilona; Fehse, Boris

    2016-03-01

    Genome editing using designer nucleases such as transcription activator-like effector nucleases (TALENs) or clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 nucleases is an emerging technology in basic and applied research. Whereas the application of editing tools, namely CRISPR-Cas9, has recently become very straightforward, quantification of resulting gene knockout rates still remains a bottleneck. This is particularly true if the product of a targeted gene is not easily detectable. To address this problem, we devised a novel gene-editing frequency digital PCR (GEF-dPCR) technique. GEF-dPCR exploits two differently labeled probes that are placed within one amplicon at the gene-editing target site to simultaneously detect wild-type and nonhomologous end-joining (NHEJ)-affected alleles. Taking advantage of the principle of dPCR, this enables concurrent quantification of edited and wild-type alleles in a given sample. We propose that our method is optimal for the monitoring of gene-edited cells in vivo, e.g., in clinical settings. Here we describe preparation, design of primers and probes, and setup and analysis of GEF-dPCR. The setup of GEF-dPCR requires up to 2 weeks (depending on the starting point); once the dPCR has been established, the protocol for sample analysis takes <1 d. PMID:26914317

  17. The RhoGEF DOCK10 is essential for dendritic spine morphogenesis

    PubMed Central

    Jaudon, Fanny; Raynaud, Fabrice; Wehrlé, Rosine; Bellanger, Jean-Michel; Doulazmi, Mohamed; Vodjdani, Guilan; Gasman, Stéphane; Fagni, Laurent; Dusart, Isabelle; Debant, Anne; Schmidt, Susanne

    2015-01-01

    By regulating actin cytoskeleton dynamics, Rho GTPases and their activators RhoGEFs are implicated in various aspects of neuronal differentiation, including dendritogenesis and synaptogenesis. Purkinje cells (PCs) of the cerebellum, by developing spectacular dendrites covered with spines, represent an attractive model system in which to decipher the molecular signaling underlying these processes. To identify novel regulators of dendritic spine morphogenesis among members of the poorly characterized DOCK family of RhoGEFs, we performed gene expression profiling of fluorescence-activated cell sorting (FACS)-purified murine PCs at various stages of their postnatal differentiation. We found a strong increase in the expression of the Cdc42-specific GEF DOCK10. Depleting DOCK10 in organotypic cerebellar cultures resulted in dramatic dendritic spine defects in PCs. Accordingly, in mouse hippocampal neurons, depletion of DOCK10 or expression of a DOCK10 GEF-dead mutant led to a strong decrease in spine density and size. Conversely, overexpression of DOCK10 led to increased spine formation. We show that DOCK10 function in spinogenesis is mediated mainly by Cdc42 and its downstream effectors N-WASP and PAK3, although DOCK10 is also able to activate Rac1. Our global approach thus identifies an unprecedented function for DOCK10 as a novel regulator of dendritic spine morphogenesis via a Cdc42-mediated pathway. PMID:25851601

  18. The Productivity and Technical Efficiency of Textile Industry Clusters in India

    NASA Astrophysics Data System (ADS)

    Bhaskaran, E.

    2013-09-01

    The Indian textile industry is one the largest and oldest sectors in the country and among the most important in the economy in terms of output, investment and employment (E). The sector employs nearly 35 million people and after agriculture, is the second-highest employer in the country. Its importance is underlined by the fact that it accounts for around 4 % of Gross Domestic Product, 14 % of industrial production, 9 % of excise collections, 18 % of E in the industrial sector, and 16 % of the country's total exports (Ex) earnings. For inclusive growth and sustainable development most of the Textile Manufacturers has adopted the Cluster Development Approach. The objective is to study the physical and financial performance, correlation, regression and Data Envelopment Analysis by measuring technical efficiency (Ø), peer weights (λi), input slacks (S-), output slacks (S+) and return to scale of four textile clusters (TCs) namely IchalKaranji Textile Cluster, Maharashtra; Ludhiana Textile Cluster, Punjab; Tirupur Textile Cluster, Tamilnadu and Panipat Textile Cluster, Haryana in India. The methodology adopted is using Data Envelopment Analysis of Output Oriented Banker Charnes Cooper Model by taking number of units (U) and number of E as inputs and sales (S) and Ex in crores as an outputs. The non-zero λi's represents the weights for efficient clusters. The S > 0 obtained for one TC reveals the excess U (S-) and E (S-) and shortage in sales (S+) and Ex (S+). To conclude, for inclusive growth and sustainable development, the inefficient TC should increase their S/turnover and Ex, as decrease in number of enterprises and E is practically not possible. Moreover for sustainable development, the TC should strengthen infrastructure interrelationships, technology interrelationships, procurement interrelationships, production interrelationships and marketing interrelationships to decrease cost, increase productivity and efficiency to compete in the world market.

  19. PIC simulation of high efficiency and high power 14 vane industrial magnetron

    NASA Astrophysics Data System (ADS)

    Vyas, Sandeep; Maurya, Shivendra; Singh, V. V. P.

    2016-03-01

    This paper presents a 3D Particle in cell (PIC) simulation of a CW 2.450±0.050 GHz 10 kW industrial magnetron. The electromagnetic and PIC simulation of magnetron has been carried out using CST microwave studio andCST particle studio. A virtual prototype of 14 vane magnetron has been simulated on computer. The cold frequency of magnetron is found 2.495 GHz. The unloaded quality factor and circuit efficiency are found 1970 and 92% from electromagnetic simulation. The output power is achieved 12.4 KW for anode voltage 12.7 kV and magnetic field 2900 Gauss. The anode current is found anode current 1.22 A. The total efficiency is 78.76 %.

  20. Improved Methods for Identifying, Applying, and Verifying Industrial Energy Efficiency Measures

    NASA Astrophysics Data System (ADS)

    Harding, Andrew Chase

    Energy efficiency is the least expensive source of additional energy capacity for today's global energy expansion. Energy efficiency offers additional benefits of cost savings for consumers, reduced environmental impacts, and enhanced energy security. The challenges of energy efficiency include identifying potential efficiency measures, quantifying savings, determining cost effectiveness, and verifying savings of installed measures. This thesis presents three separate chapters which address these challenges. The first is a paper presented at the 2014 industrial energy technology conference (IETC) that details a compressed air system project using the systems approach to identify cost effective measures, energy intensity to project savings, and proper measurement and verification (M&V) practices to prove that the savings were achieved. The second is a discussion of proper M&V techniques, how these apply to international M&V protocols, and how M&V professionals can improve the accuracy and efficacy of their M&V activities. The third is an energy intensity analysis of a poultry processing facility at a unit operations level, which details the M&V practices used to determine the intensities at each unit operation and compares these to previous works.

  1. Highly efficient industrial large-area black silicon solar cells achieved by surface nanostructured modification

    NASA Astrophysics Data System (ADS)

    Li, Ping; Wei, Yi; Zhao, Zengchao; Tan, Xin; Bian, Jiming; Wang, Yuxuan; Lu, Chunxi; Liu, Aimin

    2015-12-01

    Traditional black silicon solar cells show relatively low efficiencies due to the high surface recombination occurring at the front surfaces. In this paper, we present a surface modification process to suppress surface recombination and fabricate highly efficient industrial black silicon solar cells. The Ag-nanoparticle-assisted etching is applied to realize front surface nanostructures on silicon wafers in order to reduce the surface reflectance. Through a further tetramethylammonium hydroxide (TMAH) treatment, the carrier recombination at and near the surface is greatly suppressed, due to a lower surface dopant concentration after the surface modification. This modified surface presents a low reflectivity in a range of 350-1100 nm. Large-area solar cells with an average conversion efficiency of 19.03% are achieved by using the TMAH treatment of 30 s. This efficiency is 0.18% higher than that of standard silicon solar cells with pyramidal surfaces, and also a remarkable improvement compared with black silicon solar cells without TMAH modifications.

  2. Gas-phase chemistry of ionized and protonated GeF4: a joint experimental and theoretical study.

    PubMed

    Antoniotti, Paola; Bottizzo, Elena; Operti, Lorenza; Rabezzana, Roberto; Borocci, Stefano; Grandinetti, Felice

    2011-05-01

    The gas-phase ion chemistry of GeF(4) and of its mixtures with water, ammonia and hydrocarbons was investigated by ion trap mass spectrometry (ITMS) and ab initio calculations. Under ITMS conditions, the only fragment detected from ionized GeF(4) is GeF(3)(+). This cation is a strong Lewis acid, able to react with H(2)O, NH(3) and the unsaturated C(2)H(2), C(2)H(4) and C(6)H(6) by addition-HF elimination reactions to form F(2)Ge(XH)(+), FGe(XH)(2)(+), Ge(XH)(3)(+) (X = OH or NH(2)), F(2)GeC(2)H(+), F(2)GeC(2)H(3)(+) and F(2)GeC(6)H(5)(+). The structure, stability and thermochemistry of these products and the mechanistic aspects of the exemplary reactions of GeF(3)(+) with H(2)O, NH(3) and C(6)H(6) were investigated by MP2 and coupled cluster calculations. The experimental proton affinity (PA) and gas basicity (GB) of GeF(4) were estimated as 121.5 ± 6.0 and 117.1 ± 6.0 kcal mol(-1), respectively, and GeF(4)H(+) was theoretically characterized as an ion-dipole complex between GeF(3)(+) and HF. Consistently, it reacts with simple inorganic and organic molecules to form GeF(3)(+)-L complexes (L = H(2)O, NH(3), C(2)H(2), C(2)H(4), C(6)H(6), CO(2), SO(2) and GeF(4)). The theoretical investigation of the stability of these ions with respect to GeF(3)(+) and L disclosed nearly linear correlations between their dissociation enthalpies and free energies and the PA and GB of L. Comparing the behavior of GeF(3)(+) with the previously investigated CF(3)(+) and SiF(3)(+) revealed a periodically reversed order of reactivity CF(3)(+) < GeF(3)(+) < SiF(3)(+). This parallels the order of the Lewis acidities of the three cations. PMID:21500305

  3. Voluntary agreements for increasing energy-efficiency in industry: Case study of a pilot project with the steel industry in Shandong Province, China

    SciTech Connect

    Price, Lynn; Worrell, Ernst; Sinton, Jonathan; Yun, Jiang

    2003-03-01

    China faces a significant challenge in the years ahead to continue to provide essential materials and products for a rapidly-growing economy while addressing pressing environmental concerns. China's industrial sector is heavily dependent on the country's abundant, yet polluting, coal resources. While tremendous energy conservation and environmental protection achievements were realized in the industrial sector in the past, there remains a great gulf between the China's level of energy efficiency and that of the advanced countries of the world. Internationally, significant energy efficiency improvement in the industrial sector has been realized in a number of countries using an innovative policy mechanism called Voluntary Agreements. This paper describes international experience with Voluntary Agreements in the industrial sector as well as the development of a pilot program to test the use of such agreements with two steel mills in Shandong Province, China.

  4. [Research practices of conversion efficiency of resources utilization model of castoff from Chinese material medica industrialization].

    PubMed

    Duan, Jin-Ao; Su, Shu-Lan; Guo, Sheng; Liu, Pei; Qian, Da-Wei; Jiang, Shu; Zhu, Hua-Xu; Tang, Yu-Ping; Wu, Qi-Nan

    2013-12-01

    The industrialization chains and their products, which were formed from the process of the production of medicinal materials-prepared drug in pieces and deep processed product of Chinese material medica (CMM) resources, have generated large benefits of social and economic. However, The large of herb-medicine castoff of "non-medicinal parts" and "rejected materials" produced inevitably during the process of Chinese medicinal resources produce and process, and the residues, waste water and waste gas were produced during the manufactured and deep processed product of CMM. These lead to the waste of resources and environmental pollution. Our previous researches had proposed the "three utilization strategies" and "three types of resources models" of herb-medicine castoff according to the different physicochemical property of resources constitutes, resources potential and utility value of herb-medicine castoff. This article focus on the conversion efficiency of resources model and analysis the ways, technologies, practices, and application in herb-medicine cast off of the conversion efficiency of resources model based on the recycling economy theory of resources and thoughts of resources chemistry of CMM. These data may be promote and resolve the key problems limited the industrialization of Chinese material medica for long time and promote the realization of herb-medicine castoff resources utilization. PMID:24791476

  5. Resource acquisition, distribution and end-use efficiencies and the growth of industrial society

    NASA Astrophysics Data System (ADS)

    Jarvis, A. J.; Jarvis, S. J.; Hewitt, C. N.

    2015-10-01

    A key feature of the growth of industrial society is the acquisition of increasing quantities of resources from the environment and their distribution for end-use. With respect to energy, the growth of industrial society appears to have been near-exponential for the last 160 years. We provide evidence that indicates that the global distribution of resources that underpins this growth may be facilitated by the continual development and expansion of near-optimal directed networks (roads, railways, flight paths, pipelines, cables etc.). However, despite this continual striving for optimisation, the distribution efficiencies of these networks must decline over time as they expand due to path lengths becoming longer and more tortuous. Therefore, to maintain long-term exponential growth the physical limits placed on the distribution networks appear to be counteracted by innovations deployed elsewhere in the system, namely at the points of acquisition and end-use of resources. We postulate that the maintenance of the growth of industrial society, as measured by global energy use, at the observed rate of ~ 2.4 % yr-1 stems from an implicit desire to optimise patterns of energy use over human working lifetimes.

  6. Energy efficiency in pumping systems: Experience and trends in pulp and paper industry

    SciTech Connect

    Tutterow, V.

    1999-07-01

    Approximately two-thirds of all US industrial electricity consumption is by electric motors. Pumping systems account for an estimated 27% of this electrical motor consumption. A recent study funded by the US Department of Energy estimates that optimizing the energy efficiency of these pumping systems could reduce consumption by over 20,000 GWh/year using existing, proven techniques and technologies. This study suggests that 22% of the pumping systems savings potential lies within the pulp and paper industry. Energy use analysis being conducted by the US Department of Energy is leading to identification and quantification of savings opportunities within pulp and paper mills. Additionally, Lawrence Berkeley National Laboratory efforts over the past two years have involved extensive contact with the pulp and paper community on motor systems-related issues for the US Department of Energy's Motor Challenge program. This experience has provided an understanding of mill opportunities for improvement through contact with a mill operating personnel throughout the US. This paper examines the applications of pumping systems in the pulp and paper industry, and identifies the most common energy optimization techniques implemented to data. Options such as adjustable speed drives, impeller trimming and multiple pumping arrangements are discussed.

  7. Bonneville Power Administration and the Industrial Technologies Program Leverage Support to Overcome Energy Efficiency Barriers in the Northwest

    SciTech Connect

    2010-06-18

    Through its Energy Smart Industrial program, BPA is informing and assisting utilities and industries to have a better understanding of the benefits that come from participating in energy-savings programs. Read about how BPA is encouraging energy efficiency projects through its utilities.

  8. Industry perspectives on increasing the efficiency of coal-fired power generation

    SciTech Connect

    Torrens, I.M.; Stenzel, W.C.

    1997-12-31

    Independent power producers will build a substantial fraction of expected new coal-fired power generation in developing countries over the coming decades. To reduce perceived risk and obtain financing for their projects, they are currently building and plan to continue to build subcritical coal-fired plants with generating efficiency below 40%. Up-to-date engineering assessment leads to the conclusion that supercritical generating technology, capable of efficiencies of up to 45%, can produce electricity at a lower total cost than conventional plants. If such plants were built in Asia over the coming decades, the savings in carbon dioxide emissions over their lifetime would be measured in billions of tons. IPPs perceive supercritical technology as riskier and higher cost than conventional technology. The truth needs to be confirmed by discussions with additional experienced power engineering companies. Better communication among the interested parties could help to overcome the IPP perception issue. Governments working together with industry might be able to identify creative financing arrangements which can encourage the use of more efficient pulverized clean coal technologies, while awaiting the commercialization of advanced clean coal technologies like gasification combined cycle and pressurized fluidized bed combustion.

  9. Making industrial energy efficiency mainstream and profitable: Where public benefit and private interests intersect

    SciTech Connect

    McKane, Aimee T.; Tutterow, Vestal; Cockrill, Chris

    2001-05-31

    In 1996, the US Department of Energy s Office of Industrial Technologies (OIT) Motor Challenge program began a unique collaboration with industry called the Allied Partner program. Partnerships were sought with equipment suppliers and manufacturers, utilities, consultants, and state agencies that had extensive existing relationships with industrial customers. Partners were neither paid nor charged a fee for participation. The assumption was that these relationships could serve as the foundation for conveying a motor system efficiency message to many more industrial facilities than could be reached through a typical government-to-end-user program model. A substantial effort was made to engage industrial suppliers in delivering program information as part of their customer interactions. A recent independent evaluation of the Motor Challenge program attributes $16.9 million or nearly 67 percent of the total annual program energy savings to the efforts of Allied Partners in the first three years of operation.In 1997, the Compressed Air Challenge(R) (CAC) was developed as an outgrowth of the partnership concept. In this model, OIT is one of 15 sponsors who collaborated to create a national program of compressed air system training. The CAC has gone a step further by setting up a development and deployment model based on shared interests and shared costs among public, private, and not-for-profit organizations that serve industrial customers. Since the first CAC training session in 1999, approximately 3800 people have been trained by CAC qualified instructors--both end users and suppliers. More impressively, the entire compressed air market has begun to shift from a component-based to a system-based approach, largely as the result of collaboration. The typical leverage for OIT participation in a CAC training session is 10:1. During the past year, OIT has reorganized to integrate all of its near-term industrial offerings such as the Motor, Compressed Air, and Steam

  10. Evaluation of Efficiency Activities in the Industrial Sector Undertaken in Response to Greenhouse Gas Emission Reduction Targets

    SciTech Connect

    Price, Lynn; de la Rue du Can, Stephane; Lu, Hongyou; Horvath, Arpad

    2010-05-21

    The 2006 California Global Warming Solutions Act calls for reducing greenhouse gas (GHG) emissions to 1990 levels by 2020. Meeting this target will require action from all sectors of the California economy, including industry. The industrial sector consumes 25% of the energy used and emits 28% of the carbon dioxide (CO{sub 2}) produced in the state. Many countries around the world have national-level GHG reduction or energy-efficiency targets, and comprehensive programs focused on implementation of energy efficiency and GHG emissions mitigation measures in the industrial sector are essential for achieving their goals. A combination of targets and industry-focused supporting programs has led to significant investments in energy efficiency as well as reductions in GHG emissions within the industrial sectors in these countries. This project has identified program and policies that have effectively targeted the industrial sector in other countries to achieve real energy and CO{sub 2} savings. Programs in Ireland, France, The Netherlands, Denmark, and the UK were chosen for detailed review. Based on the international experience documented in this report, it is recommended that companies in California's industrial sector be engaged in a program to provide them with support to meet the requirements of AB32, The Global Warming Solution Act. As shown in this review, structured programs that engage industry, require members to evaluate their potential efficiency measures, plan how to meet efficiency or emissions reduction goals, and provide support in achieving the goals, can be quite effective at assisting companies to achieve energy efficiency levels beyond those that can be expected to be achieved autonomously.

  11. Mechanistic insight into GPCR-mediated activation of the microtubule-associated RhoA exchange factor GEF-H1.

    PubMed

    Meiri, David; Marshall, Christopher B; Mokady, Daphna; LaRose, Jose; Mullin, Michael; Gingras, Anne-Claude; Ikura, Mitsuhiko; Rottapel, Robert

    2014-01-01

    The RhoGEF GEF-H1 can be sequestered in an inactive state on polymerized microtubules by the dynein motor light-chain Tctex-1. Phosphorylation of GEF-H1 Ser885 by PKA or PAK kinases creates an inhibitory 14-3-3-binding site. Here we show a new mode of GEF-H1 activation in response to the G-protein-coupled receptor (GPCR) ligands lysophosphatidic acid (LPA) or thrombin that is independent of microtubule depolymerization. LPA/thrombin stimulates disassembly of the GEF-H1:dynein multi-protein complex through the concerted action of Gα and Gβγ. Gα binds directly to GEF-H1 and displaces it from Tctex-1, while Gβγ binds to Tctex-1 and disrupts its interaction with the dynein intermediate chain, resulting in the release of GEF-H1. Full activation of GEF-H1 requires dephosphorylation of Ser885 by PP2A, which is induced by thrombin. The coordinated displacement of GEF-H1 from microtubules by G-proteins and its dephosphorylation by PP2A demonstrate a multistep GEF-H1 activation and present a unique mechanism coupling GPCR signalling to Rho activation. PMID:25209408

  12. High Efficiency Liquid-Desiccant Regenerator for Air Conditioning and Industrial Drying

    SciTech Connect

    Andrew Lowenstein

    2005-12-19

    Over 2 quads of fossil fuels are used each year for moisture removal. This includes industrial and agricultural processes where feedstocks and final products must be dried, as well as comfort conditioning of indoor spaces where the control of humidity is essential to maintaining healthy, productive and comfortable working conditions. Desiccants, materials that have a high affinity for water vapor, can greatly reduce energy use for both drying and dehumidification. An opportunity exists to greatly improve the competitiveness of advanced liquid-desiccant systems by increasing the efficiency of their regenerators. It is common practice within the chemical process industry to use multiple stage boilers to improve the efficiency of thermal separation processes. The energy needed to regenerate a liquid desiccant, which is a thermal separation process, can also be reduced by using a multiple stage boiler. In this project, a two-stage regenerator was developed in which the first stage is a boiler and the second stage is a scavenging-air regenerator. The only energy input to this regenerator is the natural gas that fires the boiler. The steam produced in the boiler provides the thermal energy to run the second-stage scavenging-air regenerator. This two-stage regenerator is referred to as a 1?-effect regenerator. A model of the high-temperature stage of a 1?-effect regenerator for liquid desiccants was designed, built and successfully tested. At nominal operating conditions (i.e., 2.35 gpm of 36% lithium chloride solution, 307,000 Btu/h firing rate), the boiler removed 153 lb/h of water from the desiccant at a gas-based efficiency of 52.9 % (which corresponds to a COP of 0.95 when a scavenging-air regenerator is added). The steam leaving the boiler, when condensed, had a solids concentration of less than 10 ppm. This low level of solids in the condensate places an upper bound of about 6 lb per year for desiccant loss from the regenerator. This low loss will not create

  13. Cationic germanium fluorides: 2. An experimental and theoretical investigation on the reaction between GeF+ and H2O [1

    NASA Astrophysics Data System (ADS)

    Antoniotti, Paola; Rabezzana, Roberto; Borocci, Stefano; Bronzolino, Nicoletta; Giordani, Maria; Grandinetti, Felice

    2006-11-01

    The reaction between singlet ground state GeF+ (1[Sigma]+) and H2O, with formation of Ge-OH+ and HF, was investigated by ion trap mass spectrometry (ITMS) and ab initio calculations at the MP2 and CCSD(T) level of theory. The process involves the initial formation of the addition product FGe+-OH2 (1), its interconversion into HOGe+-FH (2), and the eventual barrier-free dissociation into Ge-OH+ and HF. The activation barrier of the 1 --> 2 isomerization is comparable with the exothermicity (exoergicity) of the formation of 1, and this explains the relatively low efficiency of the process, experimentally evaluated as 0.14 from the ratio of the absolute rate constant, 3.2 x 10-10 cm3 molecule-1 s-1, and the collisional rate constant. The mechanism of the reaction between GeF+ and H2O is strictly analogue to that of the reaction between SiF+ and H2O, investigated so far by selected-ion flow tube (SIFT) mass spectrometry and ab initio calculations. From a general point of view, the conceivable occurrence of additional similarities between the still essentially unexplored reactivity of GeFn+ (n = 1-3) and the rich and variegated chemistry of SiFn+ suggests novel attractive opportunities in the gas-phase positive ion chemistry of germanium.

  14. Xylose fermentation efficiency and inhibitor tolerance of the recombinant industrial Saccharomyces cerevisiae strain NAPX37.

    PubMed

    Li, Yun-Cheng; Mitsumasu, Kanako; Gou, Zi-Xi; Gou, Min; Tang, Yue-Qin; Li, Guo-Ying; Wu, Xiao-Lei; Akamatsu, Takashi; Taguchi, Hisataka; Kida, Kenji

    2016-02-01

    Industrial yeast strains with good xylose fermentation ability and inhibitor tolerance are important for economical lignocellulosic bioethanol production. The flocculating industrial Saccharomyces cerevisiae strain NAPX37, harboring the xylose reductase-xylitol dehydrogenase (XR-XDH)-based xylose metabolic pathway, displayed efficient xylose fermentation during batch and continuous fermentation. During batch fermentation, the xylose consumption rates at the first 36 h were similar (1.37 g/L/h) when the initial xylose concentrations were 50 and 75 g/L, indicating that xylose fermentation was not inhibited even when the xylose concentration was as high as 75 g/L. The presence of glucose, at concentrations of up to 25 g/L, did not affect xylose consumption rate at the first 36 h. Strain NAPX37 showed stable xylose fermentation capacity during continuous ethanol fermentation using xylose as the sole sugar, for almost 1 year. Fermentation remained stable at a dilution rate of 0.05/h, even though the xylose concentration in the feed was as high as 100 g/L. Aeration rate, xylose concentration, and MgSO4 concentration were found to affect xylose consumption and ethanol yield. When the xylose concentration in the feed was 75 g/L, a high xylose consumption rate of 6.62 g/L/h and an ethanol yield of 0.394 were achieved under an aeration rate of 0.1 vvm, dilution rate of 0.1/h, and 5 mM MgSO4. In addition, strain NAPX37 exhibited good tolerance to inhibitors such as weak acids, furans, and phenolics during xylose fermentation. These findings indicate that strain NAPX37 is a promising candidate for application in the industrial production of lignocellulosic bioethanol. PMID:26603762

  15. Silo effect a prominence factor to decrease efficiency of pharmaceutical industry.

    PubMed

    Vatanpour, Hossein; Khorramnia, Atoosa; Forutan, Naghmeh

    2013-01-01

    To be sure, all the industries try to be involved in globalization with a constant trend to find out ways to increase productivity across different functions within an organization to maintain competitive advantage world. Pharmaceutical industries are not exceptional and further are based on fragmentation. So these kind of companies need to cope with several barriers such as silo mentality that may affect efficiency of their business activity. Due to eliminate a part of resources such as raw materials, new molecule developed, financial and human resources and so on, companies can gradually loss their competitive potentials in the market and increase their expenses. Furthermore, to avoid any business disturbances in financially connected companies due to silo effect, they should arrange their management to integrated organization form. Otherwise, actions taken by one business member of the chain can influence the profitability of all the other members in the chain. That is why recently supply chain has generated much interest in many business units. In this paper, it has been tried to investigate the different aspects of silo effect which can affect integrate supply chain. Finally, a fluent communication, high level of information exchange, fragmentation management, cross-functional control in a supply chain management format are needed to reduce or control silo effect within entire chain of the holding company by Supply chain management. PMID:24250690

  16. Use of tracer gas technique for industrial exhaust hood efficiency evaluation--where to sample?

    PubMed

    Hampl, V; Niemelä, R; Shulman, S; Bartley, D L

    1986-05-01

    A tracer gas technique using sulfur hexafluoride (SF6) was developed for the evaluation of industrial exhaust hood efficiency. In addition to other parameters, accuracy of this method depends on proper location of the sampling probe. The sampling probe should be located in the duct at a minimum distance from the investigated hood where the SF6 is dispersed uniformly across the duct cross section. To determine the minimum sampling distance, the SF6 dispersion in the duct in fully developed turbulent flow was studied at four duct configurations frequently found in industry: straight duct, straight duct-side branch, straight duct-one elbow, and straight duct-two elbows combinations. Based on the established SF6 dispersion factor, the minimum sampling distances were determined as follows: for straight duct, at least 50 duct diameters; for straight duct-side branch combination, at least 25 duct diameters; for straight duct-one elbow combination, 7 duct diameters; and for straight duct-two elbow combination, 4 duct diameters. Sampling at (or beyond) these distances minimizes the error caused by the non-homogeneous dispersion of SF6 in the duct and contributes to the accuracy of the tracer gas technique. PMID:3717012

  17. Silo Effect a Prominence Factor to Decrease Efficiency of Pharmaceutical Industry

    PubMed Central

    Vatanpour, Hossein; Khorramnia, Atoosa; Forutan, Naghmeh

    2013-01-01

    To be sure, all the industries try to be involved in globalization with a constant trend to find out ways to increase productivity across different functions within an organization to maintain competitive advantage world. Pharmaceutical industries are not exceptional and further are based on fragmentation. So these kind of companies need to cope with several barriers such as silo mentality that may affect efficiency of their business activity. Due to eliminate a part of resources such as raw materials, new molecule developed, financial and human resources and so on, companies can gradually loss their competitive potentials in the market and increase their expenses. Furthermore, to avoid any business disturbances in financially connected companies due to silo effect, they should arrange their management to integrated organization form. Otherwise, actions taken by one business member of the chain can influence the profitability of all the other members in the chain. That is why recently supply chain has generated much interest in many business units. In this paper, it has been tried to investigate the different aspects of silo effect which can affect integrate supply chain. Finally, a fluent communication, high level of information exchange, fragmentation management, cross-functional control in a supply chain management format are needed to reduce or control silo effect within entire chain of the holding company by Supply chain management. PMID:24250690

  18. A novel SCN1A mutation associated with severe GEFS+ in a large South American pedigree.

    PubMed

    Pineda-Trujillo, N; Carrizosa, J; Cornejo, W; Arias, W; Franco, C; Cabrera, D; Bedoya, G; Ruíz-Linares, A

    2005-03-01

    Generalized epilepsy with febrile seizures plus (GEFS+) is an inherited epileptic syndrome with a marked clinical and genetic heterogeneity. Here we report the molecular characterization of a large pedigree with a severe clinical form of GEFS+. Genetic linkage analysis implied the involvement of the FEB3 in the disease phenotype of this family (parametric two-point lod-score of 2.2). Sequencing of the SCN1A gene revealed a novel aspartic acid for glycine substitution at position 1742 of this sodium channel subunit. The amino-acid replacement lies in the pore-forming region of domain IV of SCN1A. Our observations are consistent with the genotype-phenotype correlation studies suggesting that mutations in the pore-forming loop of SCN1A can lead to a clinically more severe epileptic syndrome. PMID:15694566

  19. Energy Efficient Microwave Hybrid Processing of Lime for Cement, Steel, and Glass Industries

    SciTech Connect

    Fall, Morgana L; Yakovlev, Vadim; Sahi, Catherine; Baranova, Inessa; Bowers, Johnney G; Esquenazi , Gibran L

    2012-02-10

    MAT in the US lime industry. This estimate showed that 7.3 TBTU/year could be saved, with reduction of 270 MMlbs of CO2 emissions, and $29 MM/year in economic savings. Taking into account estimates for MAT implementation in the US cement industry, an additional 39 TBTU/year, 3 Blbs of CO2 and $155 MM/year could be saved. One of the main remaining barriers to commercialization of MAT for the lime and cement industries is the sheer size of production. Through this project, it was realized that a production size MAT rotary calciner was not feasible, and a different approach was adapted. The concept of a microwave post heat section located in the upper portion of the cooler was devised and appears to be a more realistic approach for MAT implementation. Commercialization of this technology will require (1) continued pilot scale calcining demonstrations, (2) involvement of lime kiln companies, and (3) involvement of an industrial microwave equipment provider. An initial design concept for a MAT post-heat treatment section was conceived as a retrofit into the cooler sections of existing lime rotary calciners with a 1.4 year payback. Retrofitting will help spur implementation of this technology, as the capital investment will be minimal for enhancing the efficiency of current rotary lime kilns. Retrofits would likely be attractive to lime manufacturers, as the purchase of a new lime kiln is on the order of a $30 million dollar investment, where as a MAT retrofit is estimated on the order of $1 million. The path for commercialization lies in partnering with existing lime kiln companies, who will be able to implement the microwave post heat sections in existing and new build kilns. A microwave equipment provider has been identified, who would make up part of the continued development and commercialization team.

  20. The Role of Ect2 Nuclear RhoGEF Activity in Ovarian Cancer Cell Transformation

    PubMed Central

    Huff, Lauren P.; DeCristo, Molly J.; Trembath, Dimitri; Kuan, Pei Fen; Yim, Margaret; Liu, Jinsong; Cook, Danielle R.; Miller, C. Ryan; Der, Channing J.

    2013-01-01

    Ect2, a Rho guanine nucleotide exchange factor (RhoGEF), is atypical among RhoGEFs in its predominantly nuclear localization in interphase cells. One current model suggests that Ect2 mislocalization drives cellular transformation by promoting aberrant activation of cytoplasmic Rho family GTPase substrates. However, in ovarian cancers, where Ect2 is both amplified and overexpressed at the mRNA level, we observed that the protein is highly expressed and predominantly nuclear and that nuclear but not cytoplasmic Ect2 increases with advanced disease. Knockdown of Ect2 in ovarian cancer cell lines impaired their anchorage-independent growth without affecting their growth on plastic. Restoration of Ect2 expression rescued the anchorage-independent growth defect, but not if either the DH catalytic domain or the nuclear localization sequences of Ect2 were mutated. These results suggested a novel mechanism whereby Ect2 could drive transformation in ovarian cancer cells by acting as a RhoGEF specifically within the nucleus. Interestingly, Ect2 had an intrinsically distinct GTPase specificity profile in the nucleus versus the cytoplasm. Nuclear Ect2 bound preferentially to Rac1, while cytoplasmic Ect2 bound to RhoA but not Rac. Consistent with nuclear activation of endogenous Rac, Ect2 overexpression was sufficient to recruit Rac effectors to the nucleus, a process that required a functional Ect2 catalytic domain. Furthermore, expression of active nuclearly targeted Rac1 rescued the defect in transformed growth caused by Ect2 knockdown. Our work suggests a novel mechanism of Ect2-driven transformation, identifies subcellular localization as a regulator of GEF specificity, and implicates activation of nuclear Rac1 in cellular transformation. PMID:24386507

  1. Modulation of GEF-H1 Induced Signaling by Heparanase in Brain Metastatic Melanoma Cells

    PubMed Central

    Ridgway, Lon D.; Wetzel, Michael D.; Marchetti, Dario

    2010-01-01

    Mechanisms of brain metastatic melanoma (BMM) remain largely unknown. Understanding the modulation of signaling pathways that alter BMM cell invasion and metastasis is critical to develop new therapies for BMM. Heparanase has been widely implicated in cancer and is the dominant mammalian endoglycosidase which degrades heparan sulfate chains of proteoglycans (HSPG) including syndecans (SDCs). Recent findings also indicate that heparanase possesses non-enzymatic functions in its latent form. We hypothesized that extracellular heparanase modulates BMM cell signaling by involving SDC1/4 carboxy terminal—associated proteins and downstream targets. We digested BMM cell surface HS with human recombinant active or latent heparanase to delineate their effects on cytoskeletal dynamics and cell invasiveness. We identified the small GTPase guanine nucleotide exchange factor-H1 (GEF-H1) as a new component of a SDC signaling complex that is differentially expressed in BMM cells compared to corresponding non-metastatic counterparts. Second, knockdown of GEF-H1, SDC1, or SDC4 decreased BMM cell invasiveness and GEF-H1 modulated small GTPase activity of Rac1 and RhoA in conjunction with heparanase treatment. Third, both active and latent forms of heparanase affected Rac1 and RhoA activity; notably increasing RhoA activity. Both forms of heparanase were found to mediate the expression and subcellular localization of GEF-H1, and treatment of BMM with latent heparanase modulated SDC1/4 gene expression. Finally, treatment with exogenous heparanase downregulated BMM cell invasion. These studies indicate the relevance of heparanase signaling pathways in BMM progression, and provide insights into the molecular mechanisms regulating HSPG signaling in response to exogenous heparanase. PMID:20803552

  2. Efficient GO2/GH2 Injector Design: A NASA, Industry and University Cooperative Effort

    NASA Technical Reports Server (NTRS)

    Tucker, P. K.; Klem, M. D.; Fisher, S. C.; Santoro, R. J.

    1997-01-01

    Developing new propulsion components in the face of shrinking budgets presents a significant challenge. The technical, schedule and funding issues common to any design/development program are complicated by the ramifications of the continuing decrease in funding for the aerospace industry. As a result, new working arrangements are evolving in the rocket industry. This paper documents a successful NASA, industry, and university cooperative effort to design efficient high performance GO2/GH2 rocket injector elements in the current budget environment. The NASA Reusable Launch Vehicle (RLV) Program initially consisted of three vehicle/engine concepts targeted at achieving single stage to orbit. One of the Rocketdyne propulsion concepts, the RS 2100 engine, used a full-flow staged-combustion cycle. Therefore, the RS 2100 main injector would combust GO2/GH 2 propellants. Early in the design phase, but after budget levels and contractual arrangements had been set the limitations of the current gas/gas injector database were identified. Most of the relevant information was at least twenty years old. Designing high performance injectors to meet the RS 2100 requirements would require the database to be updated and significantly enhanced. However, there was no funding available to address the need for more data. NASA proposed a teaming arrangement to acquire the updated information without additional funds from the RLV Program. A determination of the types and amounts of data needed was made along with test facilities with capabilities to meet the data requirements, budget constraints, and schedule. After several iterations a program was finalized and a team established to satisfy the program goals. The Gas/Gas Injector Technology (GGIT) Program had the overall goal of increasing the ability of the rocket engine community to design efficient high-performance, durable gas/gas injectors relevant to RLV requirements. First, the program would provide Rocketdyne with data on

  3. India's iron and steel industry: Productivity, energy efficiency and carbon emissions

    SciTech Connect

    Schumacher, Katja; Sathaye, Jayant

    1998-10-01

    Historical estimates of productivity growth in India's iron and steel sector vary from indicating an improvement to a decline in the sector's productivity. The variance may be traced to the time period of study, source of data for analysis, and type of indices and econometric specifications used for reporting productivity growth. The authors derive both growth accounting and econometric estimates of productivity growth for this sector. Their results show that over the observed period from 1973--74 to 1993--94 productivity declined by 1.71{percent} as indicated by the Translog index. Calculations of the Kendrick and Solow indices support this finding. Using a translog specification the econometric analysis reveals that technical progress in India's iron and steel sector has been biased towards the use of energy and material, while it has been capital and labor saving. The decline in productivity was caused largely by the protective policy regarding price and distribution of iron and steel as well as by large inefficiencies in public sector integrated steel plants. Will these trends continue into the future, particularly where energy use is concerned? Most likely they will not. The authors examine the current changes in structure and energy efficiency undergoing in the sector. Their analysis shows that with the liberalization of the iron and steel sector, the industry is rapidly moving towards world-best technology, which will result in fewer carbon emissions and more efficient energy use in existing and future plants.

  4. Adaptation to climate change in industry: improving resource efficiency through sustainable production applications.

    PubMed

    Alkayal, Emrah; Bogurcu, Merve; Ulutas, Ferda; Demirer, Göksel Niyazi

    2015-01-01

    The objective of this study was to investigate the climate change adaptation opportunities of six companies from different sectors through resource efficiency and sustainable production. A total of 77 sustainable production options were developed for the companies based on the audits conducted. After screening these opportunities with each company's staff, 19 options were selected and implemented. Significant water savings (849,668 m3/year) were achieved as a result of the applications that targeted reduction of water use. In addition to water savings, the energy consumption was reduced by 3,607 MWh, which decreased the CO2 emissions by 904.1 tons/year. Moreover, the consumption of 278.4 tons/year of chemicals (e.g., NaCl, CdO, NaCN) was avoided, thus the corresponding pollution load to the wastewater treatment plant was reduced. Besides the tangible improvements, other gains were achieved, such as improved product quality, improved health and safety conditions, reduced maintenance requirements, and ensured compliance with national and EU regulations. To the best of the authors' knowledge, this study is the first ever activity in Turkey devoted to climate change adaptation in the private sector. This study may serve as a building block in Turkey for the integration of climate change adaptation and mitigation approach in the industry, since water efficiency (adaptation) and carbon reduction (mitigation) are achieved simultaneously. PMID:25630123

  5. Cleaning efficiency enhancement by ultrasounds for membranes used in dairy industries.

    PubMed

    Luján-Facundo, M J; Mendoza-Roca, J A; Cuartas-Uribe, B; Álvarez-Blanco, S

    2016-11-01

    Membrane cleaning is a key point for the implementation of membrane technologies in the dairy industry for proteins concentration. In this study, four ultrafiltration (UF) membranes with different molecular weight cut-offs (MWCOs) (5, 15, 30 and 50kDa) and materials (polyethersulfone and ceramics) were fouled with three different whey model solutions: bovine serum albumin (BSA), BSA plus CaCl2 and whey protein concentrate solution (Renylat 45). The purpose of the study was to evaluate the effect of ultrasounds (US) on the membrane cleaning efficiency. The influence of ultrasonic frequency and the US application modes (submerging the membrane module inside the US bath or applying US to the cleaning solution) were also evaluated. The experiments were performed in a laboratory plant which included the US equipment and the possibility of using two membrane modules (flat sheet and tubular). The fouling solution that caused the highest fouling degree for all the membranes was Renylat 45. Results demonstrated that membrane cleaning with US was effective and this effectiveness increased at lower frequencies. Although no significant differences were observed between the two different US applications modes tested, slightly higher cleaning efficiencies values placing the membrane module at the bottom of the tank were achieved. PMID:27245952

  6. Rab18 and a Rab18 GEF complex are required for normal ER structure

    PubMed Central

    Gerondopoulos, Andreas; Bastos, Ricardo Nunes; Yoshimura, Shin-ichiro; Anderson, Rachel; Carpanini, Sarah; Aligianis, Irene

    2014-01-01

    The ancestral Rab GTPase Rab18 and both subunits of the Rab3GAP complex are mutated in the human neurological and developmental disorder Warburg Micro syndrome. Here, we demonstrate that the Rab3GAP complex is a specific Rab18 guanine nucleotide exchange factor (GEF). The Rab3GAP complex localizes to the endoplasmic reticulum (ER) and is necessary for ER targeting of Rab18. It is also sufficient to promote membrane recruitment of Rab18. Disease-associated point mutations of conserved residues in either the Rab3GAP1 (T18P and E24V) or Rab3GAP2 (R426C) subunits result in loss of the Rab18 GEF and membrane-targeting activities. Supporting the view that Rab18 activity is important for ER structure, in the absence of either Rab3GAP subunit or Rab18 function, ER tubular networks marked by reticulon 4 were disrupted, and ER sheets defined by CLIMP-63 spread out into the cell periphery. Micro syndrome is therefore a disease characterized by direct loss of Rab18 function or loss of Rab18 activation at the ER by its GEF Rab3GAP. PMID:24891604

  7. Rational design of small molecule inhibitors targeting the Ras GEF, SOS1

    PubMed Central

    Evelyn, Chris R.; Duan, Xin; Biesiada, Jacek; Seibel, William L.; Meller, Jaroslaw; Zheng, Yi

    2014-01-01

    Summary Ras GTPases regulate intracellular signaling involved in cell proliferation. Elevated Ras signaling activity has been associated with human cancers. Ras activation is catalyzed by guanine-nucleotide exchange factors (GEFs), of which SOS1 is a major member that transduces receptor tyrosine kinase signaling to Ras. We have developed a rational approach coupling virtual screening with experimental screening in identifying small-molecule inhibitors targeting the catalytic site of SOS1 and SOS1-regulated Ras activity. A lead inhibitor, NSC-658497, is found to bind to SOS1, competitively suppresses SOS1-Ras interaction, and dose-dependently inhibits SOS1 GEF activity. Mutagenesis and structure-activity relationship studies map the NSC-658497 site of action to the SOS1 catalytic site, and define the chemical moieties in the inhibitor essential for the activity. NSC-658497 showed dose-dependent efficacy in inhibiting Ras, downstream signaling activities, and associated cell proliferation. These studies establish a proof of principle for rational design of small-molecule inhibitors targeting Ras GEF enzymatic activity. PMID:25455859

  8. Assessing Tropical Cyclone Forecast Skill in the GEFS Reforecast Version 2

    NASA Astrophysics Data System (ADS)

    Wang, Z.; LI, W.; Peng, M.

    2015-12-01

    The prediction skill of tropical cyclone (TC) activity in the Global Ensemble Forecasting System (GEFS) Reforecast Version-2 is evaluated. The GEFS captures the seasonality of TC genesis (TCG) reasonably well over the Atlantic. The performance of the model varies over different basins, and quantitative errors are found in the spatial distribution of TCGs. The biases in TC tracks are largely attributed to the biases in the TCG and the the large-scale steering flows. Analyzing the genesis potential index (GPI) shows that the biases in the monsoon trough and subtropical high induce the TC biases in the western North Pacific, while the southward displacement of the ITCZ leads to errors in TCG over the eastern North Pacific. The over-prediction of TCGs near the West African coast is associated with the hyperactive tropical easterly waves over the African continent. The diabatic heating rate (Q1) field in the GEFS forecasts suggests a much deeper and stronger convection than in the ERA-Interim, which can be attributed to the deficiency of model cumulus scheme. The study suggests that the better prediction of the large-scale circulation and synoptic-scale waves can help improve the TC forecasts.

  9. The regulation of RhoGEF Lfc by dynein light chain Tctex-1

    NASA Astrophysics Data System (ADS)

    Balan, Marc

    Lfc is a guanine nucleotide exchange factor (GEF) that activates the small GTPase RhoA, and its GEF activity is tightly regulated through protein-protein interactions, phosphorylation, and cellular localization. Lfc is anchored to microtubules through its interaction with the dynein light chain Tctex-1, which results in inhibition of Lfc's GEF activity. Here we present a crystallographic structure of Tctex-1 in complex with Lfc with residues 143-155 of Lfc bound at the Tctex-1 dimer interface. Structural alignment of our structure with Tctex-1 in complex with the dynein intermediate chain (DIC) shows the binding site of the DIC peptide and Lfc substantially overlap. Biochemical evidence, NMR perturbations assays and intrinsic fluorescence provide structural validation and support an extension of the Lfc binding site to the andalpha;-helices that may accommodate additional contact points with Tctex-1. We postulate a potential mechanism for Lfcandrsquo;s recruitment to the microtubules through a tripartite complex with Tctex-1 and DIC.

  10. Analysis of Energy-Efficiency Opportunities for the Cement Industry in Shandong Province, China

    SciTech Connect

    Price, Lynn; Hasanbeigi, Ali; Lu, Hongyou; Wang, Lan

    2009-10-01

    China's cement industry, which produced 1,388 million metric tons (Mt) of cement in 2008, accounts for almost half of the world's total cement production. Nearly 40% of China's cement production is from relatively obsolete vertical shaft kiln (VSK) cement plants, with the remainder from more modern rotary kiln cement plants, including plants equipped with new suspension pre-heater and pre-calciner (NSP) kilns. Shandong Province is the largest cement-producing Province in China, producing 10% of China's total cement output in 2008. This report documents an analysis of the potential to improve the energy efficiency of NSP kiln cement plants in Shandong Province. Sixteen NSP kiln cement plants were surveyed regarding their cement production, energy consumption, and current adoption of 34 energy-efficient technologies and measures. Plant energy use was compared to both domestic (Chinese) and international best practice using the Benchmarking and Energy Saving Tool for Cement (BEST-Cement). This benchmarking exercise indicated an average technical potential primary energy savings of 12% would be possible if the surveyed plants operated at domestic best practice levels in terms of energy use per ton of cement produced. Average technical potential primary energy savings of 23% would be realized if the plants operated at international best practice levels. Energy conservation supply curves for both fuel and electricity savings were then constructed for the 16 surveyed plants. Using the bottom-up electricity conservation supply curve model, the cost-effective electricity efficiency potential for the studied cement plants in 2008 is estimated to be 373 gigawatt hours (GWh), which accounts for 16% of total electricity use in the 16 surveyed cement plants in 2008. Total technical electricity-saving potential is 915 GWh, which accounts for 40% of total electricity use in the studied plants in 2008. The fuel conservation supply curve model shows the total technical fuel

  11. Energy Efficient Industrialized Housing Research Program, Center for Housing Innovation, University of Oregon and the Florida Solar Energy Center

    SciTech Connect

    Brown, G.Z.

    1990-01-01

    This research program addresses the need to increase the energy efficiency of industrialized housing. Two research centers have responsibility for the program: the Center for Housing Innovation at the University of Oregon and the Florida Solar Energy Center, a research institute of the University of Central Florida. The two organizations provide complementary architectural, systems engineering, and industrial engineering capabilities. In 1989 we worked on these tasks: (1) the formation of a steering committee, (2) the development of a multiyear research plan, (3) analysis of the US industrialized housing industry, (4) assessment of foreign technology, (5) assessment of industrial applications, (6) analysis of computerized design and evaluation tools, and (7) assessment of energy performance of baseline and advanced industrialized housing concepts. The current research program, under the guidance of a steering committee composed of industry and government representatives, focuses on three interdependent concerns -- (1) energy, (2) industrial process, and (3) housing design. Building homes in a factory offers the opportunity to increase energy efficiency through the use of new materials and processes, and to increase the value of these homes by improving the quality of their construction. Housing design strives to ensure that these technically advanced homes are marketable and will meet the needs of the people who will live in them.

  12. Industrial Compositional Streamline Simulation for Efficient and Accurate Prediction of Gas Injection and WAG Processes

    SciTech Connect

    Margot Gerritsen

    2008-10-31

    Gas-injection processes are widely and increasingly used for enhanced oil recovery (EOR). In the United States, for example, EOR production by gas injection accounts for approximately 45% of total EOR production and has tripled since 1986. The understanding of the multiphase, multicomponent flow taking place in any displacement process is essential for successful design of gas-injection projects. Due to complex reservoir geometry, reservoir fluid properties and phase behavior, the design of accurate and efficient numerical simulations for the multiphase, multicomponent flow governing these processes is nontrivial. In this work, we developed, implemented and tested a streamline based solver for gas injection processes that is computationally very attractive: as compared to traditional Eulerian solvers in use by industry it computes solutions with a computational speed orders of magnitude higher and a comparable accuracy provided that cross-flow effects do not dominate. We contributed to the development of compositional streamline solvers in three significant ways: improvement of the overall framework allowing improved streamline coverage and partial streamline tracing, amongst others; parallelization of the streamline code, which significantly improves wall clock time; and development of new compositional solvers that can be implemented along streamlines as well as in existing Eulerian codes used by industry. We designed several novel ideas in the streamline framework. First, we developed an adaptive streamline coverage algorithm. Adding streamlines locally can reduce computational costs by concentrating computational efforts where needed, and reduce mapping errors. Adapting streamline coverage effectively controls mass balance errors that mostly result from the mapping from streamlines to pressure grid. We also introduced the concept of partial streamlines: streamlines that do not necessarily start and/or end at wells. This allows more efficient coverage and avoids

  13. SynArfGEF is a guanine nucleotide exchange factor for Arf6 and localizes preferentially at post-synaptic specializations of inhibitory synapses.

    PubMed

    Fukaya, Masahiro; Kamata, Akifumi; Hara, Yoshinobu; Tamaki, Hideaki; Katsumata, Osamu; Ito, Naoki; Takeda, Shin'ichi; Hata, Yutaka; Suzuki, Tatsuo; Watanabe, Masahiko; Harvey, Robert J; Sakagami, Hiroyuki

    2011-03-01

    SynArfGEF, also known as BRAG3 or IQSEC3, is a member of the brefeldin A-resistant Arf-GEF/IQSEC family and was originally identified by screening for mRNA species associated with the post-synaptic density fraction. In this study, we demonstrate that synArfGEF activates Arf6, using Arf pull down and transferrin incorporation assays. Immunohistochemical analysis reveals that synArfGEF is present in somata and dendrites as puncta in close association with inhibitory synapses, whereas immunoelectron microscopic analysis reveals that synArfGEF localizes preferentially at post-synaptic specializations of symmetric synapses. Using yeast two-hybrid and pull down assays, we show that synArfGEF is able to bind utrophin/dystrophin and S-SCAM/MAGI-2 scaffolding proteins that localize at inhibitory synapses. Double immunostaining reveals that synArfGEF co-localizes with dystrophin and S-SCAM in cultured hippocampal neurons and cerebellar cortex, respectively. Both β-dystroglycan and S-SCAM were immunoprecipitated from brain lysates using anti-synArfGEF IgG. Taken together, these findings suggest that synArfGEF functions as a novel regulator of Arf6 at inhibitory synapses and associates with the dystrophin-associated glycoprotein complex and S-SCAM. PMID:21198641

  14. Extracellular signal regulated kinase and GEF-H1 mediate depolarization-induced Rho activation and paracellular permeability increase

    PubMed Central

    Waheed, Faiza; Speight, Pam; Kawai, Glenn; Dan, Qinghong; Kapus, András; Szászi, Katalin

    2011-01-01

    Plasma membrane depolarization activates the Rho/Rho kinase (ROK) pathway and thereby enhances myosin light chain (MLC) phosphorylation, which in turn is thought to be a key regulator of paracellular permeability. However, the upstream mechanisms that couple depolarization to Rho activation and permeability changes are unknown. Here we show that three different depolarizing stimuli (high extracellular [K+], the lipophilic cation tetraphenylphosphonium or L-alanine, which is taken up by electrogenic Na+-cotransport) all provoke robust phosphorylation of Extracellular Signal Regulated Kinase (ERK) in LLC-PK1 and MDCK cells. Importantly, inhibition of ERK prevented the depolarization-induced activation of Rho. Searching for the underlying mechanism, we have identified GEF-H1 as the ERK-regulated critical exchange factor, responsible for the depolarization-induced Rho activation. This conclusion is based on our findings that a) depolarization activated GEF-H1, but not p115RhoGEF; b) siRNA-mediated GEF-H1 silencing eliminated the activation of the Rho pathway; c) ERK inhibition prevented the activation of GEF-H1. Moreover, we found that the Na+/K+ pump inhibitor ouabain also caused ERK, GEF-H1 and Rho activation, partially due to its depolarizing effect. Regarding functional consequences of this newly identified pathway, we found that depolarization increased paracellular permeability in LLC-PK1 and MDCK cells, and this effect was mitigated by inhibiting myosin using blebbistatin or a dominant negative (phosphorylation-incompetent) MLC. Taken together, we propose, that the ERK/GEF-H1/Rho/ROK/pMLC pathway could be a central mechanism whereby electrogenic transmembrane transport processes control myosin phosphorylation and regulate paracellular transport in the tubular epithelium. PMID:20237148

  15. Technology partnerships: Enhancing the competitiveness, efficiency, and environmental quality of American industry. Executive summary

    SciTech Connect

    1995-04-01

    This document briefly describes the Department of Energy`s (DOE`s) Office of Industrial Technologies (OIT) program. It profiles the energy, economic, and environmental characteristics of OIT`s principal customers--the materials and process industries--that consume nearly 80% of all energy used by industry in the US. OIT-supported research, development, and demonstration (RD and D) activities relating to these industries are described as well as OIT`s crosscutting technology programs that target the needs of multiple US industries. Quantitative estimates of the potential benefits (or metrics) to US industry of many current OIT-supported technologies are also discussed.

  16. Efficient electric motor systems for industry. Report on roundtable discussions of market problems and ways to overcome them

    SciTech Connect

    Not Available

    1993-11-01

    Improving the efficiency of electric motor systems is one of the best energy-saving opportunities for the United States. The Department of Energy (DOE) Office of Industrial Technologies estimates that by the year 2010 in the industrial sector, the opportunities for savings from improved efficiency in electric motor systems could be roughly as follows: 240 billion kilowatthours per year. $13 billion per year from US industry`s energy bill. Up to 50,000 megawatts in new powerplant capacity avoided. Up to 44 million metric tons of carbon-equivalent emissions mitigated per year, corresponding to 3 percent of present US emissions. Recognizing the benefits of this significant opportunity for energy savings, DOE has targeted improvements in the efficiency of electric motor systems as a key initiative in the effort to promote flexibility and efficiency in the way electricity is produced and used. Efficient electric motor systems will help the United States reach its national goals for energy savings and greenhouse gas emission reductions.

  17. Voluntary Agreements for Increasing Energy-Efficiency in Industry: Case Study of a Pilot Project with the Steel Industry in Shandong Province, China

    SciTech Connect

    Price, Lynn; Worrell, Ernst; Sinton, Jonathan; Yun, Jiang

    2003-03-01

    This paper describes international experience with the use of Voluntary Agreements for increasing industrial sector energy-efficiency, drawing lessons learned regarding the essential elements of the more successful programs. The paper focuses on a pilot project for implementation of a Voluntary Agreement with two steel mills in Shandong Province that was developed through international collaboration with experts in China, the Netherlands, and the U.S. Designing the pilot project involved development of approaches for energy-efficiency potential assessments for the steel mills, target-setting to establish the Voluntary Agreement energy-efficiency goals, preparing energy-efficiency plans for implementation of energy-saving technologies and measures, and monitoring and evaluating the project's energy savings.

  18. Energy Efficiency Improvement and Cost Saving Opportunities for the Glass Industry. An ENERGY STAR Guide for Energy and Plant Managers

    SciTech Connect

    Galitsky, Christina; Worrell, Ernst; Galitsky, Christina; Masanet, Eric; Graus, Wina

    2008-03-01

    The U.S. glass industry is comprised of four primary industry segments--flat glass, container glass, specialty glass, and fiberglass--which together consume $1.6 billion in energy annually. On average, energy costs in the U.S. glass industry account for around 14 percent of total glass production costs. Energy efficiency improvement is an important way to reduce these costs and to increase predictable earnings, especially in times of high energy price volatility. There is a variety of opportunities available at individual plants in the U.S. glass industry to reduce energy consumption in a cost-effective manner. This Energy Guide discusses energy efficiency practices and energy-efficient technologies that can be implemented at the component, process, system, and organizational levels. A discussion of the trends, structure, and energy consumption characteristics of the U.S. glass industry is provided along with a description of the major process steps in glass manufacturing. Expected savings in energy and energy-related costs are given for many energy efficiency measures, based on case study data from real-world applications in glass production facilities and related industries worldwide. Typical measure payback periods and references to further information in the technical literature are also provided, when available. The information in this Energy Guide is intended to help energy and plant managers in the U.S. glass industry reduce energy consumption in a cost-effective manner while maintaining the quality of products manufactured. Further research on the economics of the measures--as well on as their applicability to different production practices--is needed to assess potential implementation of selected technologies at individual plants.

  19. Opportunities for Energy Efficiency and Demand Response in the California Cement Industry

    SciTech Connect

    Olsen, Daniel; Goli, Sasank; Faulkner, David; McKane, Aimee

    2010-12-22

    This study examines the characteristics of cement plants and their ability to shed or shift load to participate in demand response (DR). Relevant factors investigated include the various equipment and processes used to make cement, the operational limitations cement plants are subject to, and the quantities and sources of energy used in the cement-making process. Opportunities for energy efficiency improvements are also reviewed. The results suggest that cement plants are good candidates for DR participation. The cement industry consumes over 400 trillion Btu of energy annually in the United States, and consumes over 150 MW of electricity in California alone. The chemical reactions required to make cement occur only in the cement kiln, and intermediate products are routinely stored between processing stages without negative effects. Cement plants also operate continuously for months at a time between shutdowns, allowing flexibility in operational scheduling. In addition, several examples of cement plants altering their electricity consumption based on utility incentives are discussed. Further study is needed to determine the practical potential for automated demand response (Auto-DR) and to investigate the magnitude and shape of achievable sheds and shifts.

  20. An efficient and improved methodology for the screening of industrially valuable xylano-pectino-cellulolytic microbes.

    PubMed

    Singh, Avtar; Kaur, Amanjot; Dua, Anita; Mahajan, Ritu

    2015-01-01

    Xylano-pectino-cellulolytic enzymes are valuable enzymes of the industrial sector. In our earlier study, we have reported a novel and cost effective methodology for the qualitative screening of cellulase-free xylano-pectinolytic microorganisms by replacing the commercial, highly expensive substrates with agricultural residues, but the microorganisms with xylanolytic, pectinolytic, cellulolytic, xylano-pectinolytic, xylano-cellulolytic, pectino-cellulolytic, and xylano-pectino-cellulolytic potential were obtained. The probability of getting the desired combination was low, so efforts were made to further improve this cost effective methodology for obtaining the high yield of the microbes capable of producing desired combination of enzymes. By inclusion of multiple enrichment steps in sequence, using only practically low cost substrates and without any nutrient media till primary screening stage, this improved novel protocol for screening gave only the desired microorganisms with xylano-pectino-cellulolytic activity. Using this rapid, efficient, cost effective, and improved methodology, microbes with required combination of enzymes can be obtained and the probability of getting the desired microorganisms is cent percent. This is the first report presenting the methodology for the isolation of xylano-pectino-cellulolytic positive microorganisms at low cost and consuming less time. PMID:25692034

  1. An Efficient and Improved Methodology for the Screening of Industrially Valuable Xylano-Pectino-Cellulolytic Microbes

    PubMed Central

    Singh, Avtar; Kaur, Amanjot; Dua, Anita; Mahajan, Ritu

    2015-01-01

    Xylano-pectino-cellulolytic enzymes are valuable enzymes of the industrial sector. In our earlier study, we have reported a novel and cost effective methodology for the qualitative screening of cellulase-free xylano-pectinolytic microorganisms by replacing the commercial, highly expensive substrates with agricultural residues, but the microorganisms with xylanolytic, pectinolytic, cellulolytic, xylano-pectinolytic, xylano-cellulolytic, pectino-cellulolytic, and xylano-pectino-cellulolytic potential were obtained. The probability of getting the desired combination was low, so efforts were made to further improve this cost effective methodology for obtaining the high yield of the microbes capable of producing desired combination of enzymes. By inclusion of multiple enrichment steps in sequence, using only practically low cost substrates and without any nutrient media till primary screening stage, this improved novel protocol for screening gave only the desired microorganisms with xylano-pectino-cellulolytic activity. Using this rapid, efficient, cost effective, and improved methodology, microbes with required combination of enzymes can be obtained and the probability of getting the desired microorganisms is cent percent. This is the first report presenting the methodology for the isolation of xylano-pectino-cellulolytic positive microorganisms at low cost and consuming less time. PMID:25692034

  2. Ultra-High Efficiency and Low-Emissions Combustion Technology for Manufacturing Industries

    SciTech Connect

    Atreya, Arvind

    2013-04-15

    The purpose of this research was to develop and test a transformational combustion technology for high temperature furnaces to reduce the energy intensity and carbon footprint of U.S. manufacturing industries such as steel, aluminum, glass, metal casting, and petroleum refining. A new technology based on internal and/or external Flue Gas Recirculation (FGR) along with significant enhancement in flame radiation was developed. It produces "Radiative Flameless Combustion (RFC)" and offers tremendous energy efficiency and pollutant reduction benefits over and above the now popular "flameless combustion." It will reduce the energy intensity (or fuel consumption per unit system output) by more than 50% and double the furnace productivity while significantly reducing pollutants and greenhouse gas emissions (10^3 times reduction in NOx and 10 times reduction in CO & hydrocarbons and 3 times reduction in CO2). Product quality improvements are also expected due to uniform radiation, as well as, reduction in scale/dross formation is expected because of non-oxidative atmosphere. RFC is inexpensive, easy to implement, and it was successfully tested in a laboratory-scale furnace at the University of Michigan during the course of this work. A first-ever theory with gas and particulate radiation was also developed. Numerical programs were also written to design an industrial-scale furnace. Nine papers were published (or are in the process of publication). We believe that this early stage research adequately proves the concept through laboratory experiments, modeling and computational models. All this work is presented in the published papers. Important conclusions of this work are: (1) It was proved through experimental measurements that RFC is not only feasible but a very beneficial technology. (2) Theoretical analysis of RFC was done in (a) spatially uniform strain field and (b) a planar momentum jet where the strain rate is neither prescribed nor uniform. Four important non

  3. Industry

    SciTech Connect

    Bernstein, Lenny; Roy, Joyashree; Delhotal, K. Casey; Harnisch, Jochen; Matsuhashi, Ryuji; Price, Lynn; Tanaka, Kanako; Worrell, Ernst; Yamba, Francis; Fengqi, Zhou; de la Rue du Can, Stephane; Gielen, Dolf; Joosen, Suzanne; Konar, Manaswita; Matysek, Anna; Miner, Reid; Okazaki, Teruo; Sanders, Johan; Sheinbaum Parado, Claudia

    2007-12-01

    This chapter addresses past, ongoing, and short (to 2010) and medium-term (to 2030) future actions that can be taken to mitigate GHG emissions from the manufacturing and process industries. Globally, and in most countries, CO{sub 2} accounts for more than 90% of CO{sub 2}-eq GHG emissions from the industrial sector (Price et al., 2006; US EPA, 2006b). These CO{sub 2} emissions arise from three sources: (1) the use of fossil fuels for energy, either directly by industry for heat and power generation or indirectly in the generation of purchased electricity and steam; (2) non-energy uses of fossil fuels in chemical processing and metal smelting; and (3) non-fossil fuel sources, for example cement and lime manufacture. Industrial processes also emit other GHGs, e.g.: (1) Nitrous oxide (N{sub 2}O) is emitted as a byproduct of adipic acid, nitric acid and caprolactam production; (2) HFC-23 is emitted as a byproduct of HCFC-22 production, a refrigerant, and also used in fluoroplastics manufacture; (3) Perfluorocarbons (PFCs) are emitted as byproducts of aluminium smelting and in semiconductor manufacture; (4) Sulphur hexafluoride (SF{sub 6}) is emitted in the manufacture, use and, decommissioning of gas insulated electrical switchgear, during the production of flat screen panels and semiconductors, from magnesium die casting and other industrial applications; (5) Methane (CH{sub 4}) is emitted as a byproduct of some chemical processes; and (6) CH{sub 4} and N{sub 2}O can be emitted by food industry waste streams. Many GHG emission mitigation options have been developed for the industrial sector. They fall into three categories: operating procedures, sector-wide technologies and process-specific technologies. A sampling of these options is discussed in Sections 7.2-7.4. The short- and medium-term potential for and cost of all classes of options are discussed in Section 7.5, barriers to the application of these options are addressed in Section 7.6 and the implication of

  4. Activation of G proteins by GIV-GEF is a pivot point for insulin resistance and sensitivity

    PubMed Central

    Ma, Gary S.; Lopez-Sanchez, Inmaculada; Aznar, Nicolas; Kalogriopoulos, Nicholas; Pedram, Shabnam; Midde, Krishna; Ciaraldi, Theodore P.; Henry, Robert R.; Ghosh, Pradipta

    2015-01-01

    Insulin resistance (IR) is a metabolic disorder characterized by impaired insulin signaling and cellular glucose uptake. The current paradigm for insulin signaling centers upon the insulin receptor (InsR) and its substrate IRS1; the latter is believed to be the sole conduit for postreceptor signaling. Here we challenge that paradigm and show that GIV/Girdin, a guanidine exchange factor (GEF) for the trimeric G protein Gαi, is another major hierarchical conduit for the metabolic insulin response. By virtue of its ability to directly bind InsR, IRS1, and phosphoinositide 3-kinase, GIV serves as a key hub in the immediate postreceptor level, which coordinately enhances the metabolic insulin response and glucose uptake in myotubes via its GEF function. Site-directed mutagenesis or phosphoinhibition of GIV-GEF by the fatty acid/protein kinase C-theta pathway triggers IR. Insulin sensitizers reverse phosphoinhibition of GIV and reinstate insulin sensitivity. We also provide evidence for such reversible regulation of GIV-GEF in skeletal muscles from patients with IR. Thus GIV is an essential upstream component that couples InsR to G-protein signaling to enhance the metabolic insulin response, and impairment of such coupling triggers IR. We also provide evidence that GIV-GEF serves as therapeutic target for exogenous manipulation of physiological insulin response and reversal of IR in skeletal muscles. PMID:26378251

  5. Activation of G proteins by GIV-GEF is a pivot point for insulin resistance and sensitivity.

    PubMed

    Ma, Gary S; Lopez-Sanchez, Inmaculada; Aznar, Nicolas; Kalogriopoulos, Nicholas; Pedram, Shabnam; Midde, Krishna; Ciaraldi, Theodore P; Henry, Robert R; Ghosh, Pradipta

    2015-11-15

    Insulin resistance (IR) is a metabolic disorder characterized by impaired insulin signaling and cellular glucose uptake. The current paradigm for insulin signaling centers upon the insulin receptor (InsR) and its substrate IRS1; the latter is believed to be the sole conduit for postreceptor signaling. Here we challenge that paradigm and show that GIV/Girdin, a guanidine exchange factor (GEF) for the trimeric G protein Gαi, is another major hierarchical conduit for the metabolic insulin response. By virtue of its ability to directly bind InsR, IRS1, and phosphoinositide 3-kinase, GIV serves as a key hub in the immediate postreceptor level, which coordinately enhances the metabolic insulin response and glucose uptake in myotubes via its GEF function. Site-directed mutagenesis or phosphoinhibition of GIV-GEF by the fatty acid/protein kinase C-theta pathway triggers IR. Insulin sensitizers reverse phosphoinhibition of GIV and reinstate insulin sensitivity. We also provide evidence for such reversible regulation of GIV-GEF in skeletal muscles from patients with IR. Thus GIV is an essential upstream component that couples InsR to G-protein signaling to enhance the metabolic insulin response, and impairment of such coupling triggers IR. We also provide evidence that GIV-GEF serves as therapeutic target for exogenous manipulation of physiological insulin response and reversal of IR in skeletal muscles. PMID:26378251

  6. Crystal structure of hGEF-H1 PH domain provides insight into incapability in phosphoinositide binding.

    PubMed

    Jiang, Yan; Jiang, Heli; Zhou, Shaoyang; Meng, Bing; Liu, Zhi-Jie; Ouyang, Songying

    2016-03-18

    The guanine nucleotide exchange factor GEF-H1 (also known as ARHGEF2) is identified as a member of the Dbl family of GEFs. It regulates RhoA-dependent cell signaling pathways and plays important roles in biological processes. GEF-H1 contains an N-terminal zinc finger domain, a Dbl-homologous (DH) domain followed by a Pleckstrin homology (PH) domain, and a C-terminal domain. The specific roles of its PH domain are poorly understood. Here we report the crystal structure of human GEF-H1 PH domain to 2.45 Å resolution. A conserved surface is formed by β8, β9, β10, and it may mediate protein-protein interactions. Although the folding resembles other PH domains that have defined structures, superposition of different PH domains clearly shows that the loop between β6/β7 and the loop between β3/β4 are so close that they will prevent its binding with phosphoinositide due to steric hindrance, and this has been proved by isothermal titration calorimetry (ITC) and thermal shift assay (TSA). Our studies provide a structural framework for further work on the function of GEF-H1. PMID:26820534

  7. Energy-Efficiency and Air-Pollutant Emissions-Reduction Opportunities for the Ammonia Industry in China

    SciTech Connect

    Ma, Ding; Hasanbeigi, Ali; Chen, Wenying

    2015-06-01

    As one of the most energy-intensive and polluting industries, ammonia production is responsible for significant carbon dioxide (CO2) and air-pollutant emissions. Although many energy-efficiency measures have been proposed by the Chinese government to mitigate greenhouse gas emissions and improve air quality, lack of understanding of the cost-effectiveness of such improvements has been a barrier to implementing these measures. Assessing the costs, benefits, and cost-effectiveness of different energy-efficiency measures is essential to advancing this understanding. In this study, a bottom-up energy conservation supply curve model is developed to estimate the potential for energy savings and emissions reductions from 26 energy-efficiency measures that could be applied in China’s ammonia industry. Cost-effective implementation of these measures saves a potential 271.5 petajoules/year for fuel and 5,443 gigawatt-hours/year for electricity, equal to 14% of fuel and 14% of electricity consumed in China’s ammonia industry in 2012. These reductions could mitigate 26.7 million tonnes of CO2 emissions. This study also quantifies the co-benefits of reducing air-pollutant emissions and water use that would result from saving energy in China’s ammonia industry. This quantitative analysis advances our understanding of the cost-effectiveness of energy-efficiency measures and can be used to augment efforts to reduce energy use and environmental impacts.

  8. Energy efficiency business options for industrial end users in Latin American competitive energy markets: The case of Colombia

    NASA Astrophysics Data System (ADS)

    Botero, Sergio

    2002-01-01

    Energy markets today in Latin America and worldwide are being restructured from monopolies, either state-owned or privately-owned, to be more openly competitive and incorporate more participation from the private sector. Thus, the schemes that were formerly developed to foster end use energy efficiency are no longer applicable because they were based on mandatory regulations made with political decisions, without sufficiently considering economic feasibility. A consensus exists that the only way energy efficiency could survive in this new paradigm is by being market oriented, giving better services, and additional options to users. However; there is very little information on what end users prefer, and which options would most satisfy customers. Using Colombia as a case study, this research determines and categorizes the energy efficiency business options for large energy end users that can freely participate in the competitive energy market. The energy efficiency market is understood as a market of services aiming to increase efficiency in energy use. These services can be grouped into seven business options. A survey, following the descriptive method, was sent to energy end users in order to determine their preferences for specific energy efficiency business options, as well as the decision-making criteria taken into account for such options. This data was categorized in ten industry groups. As a conclusion, energy efficiency providers should adapt not only to the economic activity or processes of each customer, but also to the potential business options. It was also found that not all industries consider performance contracting as their most preferred option, as a matter of fact, some industries show much higher preference for conventional business options. Among end users, the divergence in option preferences contrasted with the convergence in decision-making criteria. The decision-making criteria "cost-benefit ratio" overwhelmed all other criterion. End users

  9. Industrial innovations for tomorrow: Advances in industrial energy-efficiency technologies. Degradable plastic made from potato peels

    SciTech Connect

    Not Available

    1992-07-01

    Stimulated by public demand and state and federal legislation, industry has begun to develop bio- and photo- degradable plastics. so far, however, none of these degradable plastics meets all of the criteria for success - adequate physical and mechanical properties for the desired use, cost-effectiveness, and 100% degradability. Polylactic acid (PLA) plastic is one degradable plastic that shows promise. It has the desired properties and is 100% degradable. However, PLA plastic made by conventional techniques is not cost effective. Made from lactic acid, which is typically made form petroleum using a very costly synthesis process. Lactic acid can also be made from carbohydrates (starches), found in food processing wastes such as potato wastes, cheese whey, and sorghum. Conversion of starch to simple sugars, and fermentation of these sugars can produce lactic acid.

  10. International Experience with Key Program Elements of IndustrialEnergy Efficiency or Greenhouse Gas Emissions Reduction Target-SettingPrograms

    SciTech Connect

    Price, Lynn; Galitsky, Christina; Kramer, Klaas Jan

    2008-02-02

    Target-setting agreements, also known as voluntary ornegotiated agreements, have been used by a number of governments as amechanism for promoting energy efficiency within the industrial sector. Arecent survey of such target-setting agreement programs identified 23energy efficiency or GHG emissions reduction voluntary agreement programsin 18 countries. International best practice related to target-settingagreement programs calls for establishment of a coordinated set ofpolicies that provide strong economic incentives as well as technical andfinancial support to participating industries. The key program elementsof a target-setting program are the target-setting process,identification of energy-saving technologies and measures usingenergy-energy efficiency guidebooks and benchmarking as well as byconducting energy-efficiency audits, development of an energy-savingsaction plan, development and implementation of energy managementprotocols, development of incentives and supporting policies, monitoringprogress toward targets, and program evaluation. This report firstprovides a description of three key target-setting agreement programs andthen describes international experience with the key program elementsthat comprise such programs using information from the three keytarget-setting programs as well as from other international programsrelated to industrial energy efficiency or GHG emissionsreductions.

  11. KlROM2 encodes an essential GEF homologue in Kluyveromyces lactis.

    PubMed

    Lorberg, Anja; Schmitz, Hans-Peter; Gengenbacher, Ute; Heinisch, Jürgen J

    2003-05-01

    Cellular integrity in yeasts is ensured by a rigid cell wall whose synthesis is controlled by a MAP kinase signal transduction cascade. In Saccharomyces cerevisiae upstream regulatory components of this MAP kinase pathway involve a single protein kinase C, which is regulated in part by interaction with the small GTPase Rho1p. This small G protein is in turn rendered inactive (GDP-bound) or is activated (GTP-bound) by the influence of GTPase activating proteins (GAPs) and the GDP/GTP exchange factors (GEFs), respectively. We report here on the isolation of a gene from Kluyveromyces lactis, KlROM2, which encodes a member of the latter protein family. The nucleotide sequence contains an open reading frame of 1227 amino acids, with an overall identity of 57% to the Rom2 protein of S. cerevisiae. Four conserved sequence motifs could be identified: a RhoGEF domain, a DEP sequence, a CNH domain and a less conserved pleckstrin homology (PH) sequence. Klrom2 null mutants show a lethal phenotype, which indicates that the gene may encode the only functional GEF regulating the cellular integrity pathway in K. lactis. Conditional genomic expression of KlROM2 resulted in sensitivity towards caffeine and Calcofluor white as typical phenotypes of mutants defective in this pathway. Overexpression of KIROM2 from multicopy plasmids under the control of the ScGAL1 promoter severely impaired growth in both S. cerevisiae and in K. lactis. The fact that the lethal phenotype was not prevented in mpk1 deletion mutants indicates that growth inhibition is not simply caused by hyperactivation of the Pkc1p signal transduction pathway. PMID:12734799

  12. Which Way In? The RalF Arf-GEF Orchestrates Rickettsia Host Cell Invasion

    PubMed Central

    Rennoll-Bankert, Kristen E.; Rahman, M. Sayeedur; Gillespie, Joseph J.; Guillotte, Mark L.; Kaur, Simran J.; Lehman, Stephanie S.; Beier-Sexton, Magda; Azad, Abdu F.

    2015-01-01

    Bacterial Sec7-domain-containing proteins (RalF) are known only from species of Legionella and Rickettsia, which have facultative and obligate intracellular lifestyles, respectively. L. pneumophila RalF, a type IV secretion system (T4SS) effector, is a guanine nucleotide exchange factor (GEF) of ADP-ribosylation factors (Arfs), activating and recruiting host Arf1 to the Legionella-containing vacuole. In contrast, previous in vitro studies showed R. prowazekii (Typhus Group) RalF is a functional Arf-GEF that localizes to the host plasma membrane and interacts with the actin cytoskeleton via a unique C-terminal domain. As RalF is differentially encoded across Rickettsia species (e.g., pseudogenized in all Spotted Fever Group species), it may function in lineage-specific biology and pathogenicity. Herein, we demonstrate RalF of R. typhi (Typhus Group) interacts with the Rickettsia T4SS coupling protein (RvhD4) via its proximal C-terminal sequence. RalF is expressed early during infection, with its inactivation via antibody blocking significantly reducing R. typhi host cell invasion. For R. typhi and R. felis (Transitional Group), RalF ectopic expression revealed subcellular localization with the host plasma membrane and actin cytoskeleton. Remarkably, R. bellii (Ancestral Group) RalF showed perinuclear localization reminiscent of ectopically expressed Legionella RalF, for which it shares several structural features. For R. typhi, RalF co-localization with Arf6 and PI(4,5)P2 at entry foci on the host plasma membrane was determined to be critical for invasion. Thus, we propose recruitment of PI(4,5)P2 at entry foci, mediated by RalF activation of Arf6, initiates actin remodeling and ultimately facilitates bacterial invasion. Collectively, our characterization of RalF as an invasin suggests that, despite carrying a similar Arf-GEF unknown from other bacteria, different intracellular lifestyles across Rickettsia and Legionella species have driven divergent roles for Ral

  13. Combining inhibitor tolerance and D-xylose fermentation in industrial Saccharomyces cerevisiae for efficient lignocellulose-based bioethanol production

    PubMed Central

    2013-01-01

    Background In addition to efficient pentose utilization, high inhibitor tolerance is a key trait required in any organism used for economically viable industrial bioethanol production with lignocellulose biomass. Although recent work has succeeded in establishing efficient xylose fermentation in robust industrial Saccharomyces cerevisiae strains, the resulting strains still lacked sufficient inhibitor tolerance for efficient sugar fermentation in lignocellulose hydrolysates. The aim of the present work was to combine high xylose fermentation activity and high inhibitor tolerance in a single industrial yeast strain. Results We have screened 580 yeast strains for high inhibitor tolerance using undetoxified acid-pretreated spruce hydrolysate and identified a triploid industrial baker’s yeast strain as having the highest inhibitor tolerance. From this strain, a mating competent diploid segregant with even higher inhibitor tolerance was obtained. It was crossed with the recently developed D-xylose fermenting diploid industrial strain GS1.11-26, with the Ethanol Red genetic background. Screening of 819 diploid segregants from the tetraploid hybrid resulted in two strains, GSF335 and GSF767, combining high inhibitor tolerance and efficient xylose fermentation. In a parallel approach, meiotic recombination of GS1.11-26 with a haploid segregant of Ethanol Red and screening of 104 segregants resulted in a similar inhibitor tolerant diploid strain, GSE16. The three superior strains exhibited significantly improved tolerance to inhibitors in spruce hydrolysate, higher glucose consumption rates, higher aerobic growth rates and higher maximal ethanol accumulation capacity in very-high gravity fermentation, compared to GS1.11-26. In complex medium, the D-xylose utilization rate by the three superior strains ranged from 0.36 to 0.67 g/g DW/h, which was lower than that of GS1.11-26 (1.10 g/g DW/h). On the other hand, in batch fermentation of undetoxified acid-pretreated spruce

  14. Voluntary Agreements for Energy Efficiency or GHG EmissionsReduction in Industry: An Assessment of Programs Around the World

    SciTech Connect

    Price, Lynn

    2005-06-01

    Voluntary agreements for energy efficiency improvement and reduction of energy-related greenhouse gas (GHG) emissions have been a popular policy instrument for the industrial sector in industrialized countries since the 1990s. A number of these national-level voluntary agreement programs are now being modified and strengthened, while additional countries--including some recently industrialized and developing countries--are adopting these type of agreements in an effort to increase the energy efficiency of their industrial sectors.Voluntary agreement programs can be roughly divided into three broad categories: (1) programs that are completely voluntary, (2) programs that use the threat of future regulations or energy/GHG emissions taxes as a motivation for participation, and (3) programs that are implemented in conjunction with an existing energy/GHG emissions tax policy or with strict regulations. A variety of government-provided incentives as well as penalties are associated with these programs. This paper reviews 23 energy efficiency or GHG emissions reduction voluntary agreement programs in 18 countries, including countries in Europe, the U.S., Canada, Australia, New Zealand, Japan, South Korea, and Chinese Taipei (Taiwan) and discusses preliminary lessons learned regarding program design and effectiveness. The paper notes that such agreement programs, in which companies inventory and manage their energy use and GHG emissions to meet specific reduction targets, are an essential first step towards GHG emissions trading programs.

  15. Cloning and characterization of GEF-H1, a microtubule-associated guanine nucleotide exchange factor for Rac and Rho GTPases.

    PubMed

    Ren, Y; Li, R; Zheng, Y; Busch, H

    1998-12-25

    The Rho-related small GTPases are critical elements involved in regulation of signal transduction cascades from extracellular stimuli to cell nucleus and cytoskeleton. The Dbl-like guanine nucleotide exchange factors (GEF) have been implicated in direct activation of these GTPases. Here we have identified a new member of the Dbl family, GEF-H1, by screening a human HeLa cell cDNA library. GEF-H1 encodes a 100-kDa protein containing the conserved structural array of a Dbl homology domain in tandem with a pleckstrin homology domain and is most closely related to the lfc oncogene, but additionally it contains a unique coiled-coil domain at the carboxyl terminus. Biochemical analysis reveals that GEF-H1 is capable of stimulating guanine nucleotide exchange of Rac and Rho but is inactive toward Cdc42, TC10, or Ras. Moreover, GEF-H1 binds to Rac and Rho proteins in both the GDP- and guanosine 5'-3-O-(thio)triphosphate-bound states without detectable affinity for Cdc42 or Ras. Immunofluorescence reveals that GEF-H1 colocalizes with microtubules through the carboxyl-terminal coiled-coil domain. Overexpression of GEF-H1 in COS-7 cells results in induction of membrane ruffles. These results suggest that GEF-H1 may have a direct role in activation of Rac and/or Rho and in bringing the activated GTPase to specific target sites such as microtubules. PMID:9857026

  16. Dimensions of Effectiveness and Efficiency: A Case Study on Industry-School Partnerships

    ERIC Educational Resources Information Center

    Pillay, Hitendra; Watters, James J.; Hoff, Lutz; Flynn, Matthew

    2014-01-01

    Internationally, the delivery of vocational education and training is being challenged by increasing skills shortages in certain industries and/or rapidly changing skill requirements. To respond to this challenge, rigid and centralised state bureaucracies are increasingly adopting partnerships between schools and industry as a strategy to…

  17. Energy Efficiency Improvement and Cost Saving Opportunities for the Pharmaceutical Industry. An ENERGY STAR Guide for Energy and Plant Managers

    SciTech Connect

    Galitsky, Christina; Galitsky, Christina; Chang, Sheng-chieh; Worrell, Ernst; Masanet, Eric

    2008-03-01

    The U.S. pharmaceutical industry consumes almost $1 billion in energy annually. Energy efficiency improvement is an important way to reduce these costs and to increase predictable earnings, especially in times of high energy price volatility. There are a variety of opportunities available at individual plants in the U.S. pharmaceutical industry to reduce energy consumption in a cost-effective manner. This Energy Guide discusses energy efficiency practices and energy efficient technologies that can be implemented at the component, process, system, and organizational levels. A discussion of the trends, structure, and energy consumption characteristics of the U.S. pharmaceutical industry is provided along with a description of the major process steps in the pharmaceutical manufacturing process. Expected savings in energy and energy-related costs are given for many energy efficiency measures, based on case study data from real-world applications in pharmaceutical and related facilities worldwide. Typical measure payback periods and references to further information in the technical literature are also provided, when available. The information in this Energy Guide is intended to help energy and plant managers reduce energy consumption in a cost-effective manner while meeting regulatory requirements and maintaining the quality of products manufactured. At individual plants, further research on the economics of the measures?as well as their applicability to different production practices?is needed to assess potential implementation of selected technologies.

  18. Gas prices and fuel efficiency in the U.S. automobile industry: Policy implications of endogenous product choice

    NASA Astrophysics Data System (ADS)

    Gramlich, Jacob Pleune

    I develop, estimate, and utilize an economic model of the U.S. automobile industry. I do so to address policy questions concerning automotive fuel efficiency (the relationship between gasoline used and distance traveled). Fuel efficiency has played a prominent role in our domestic energy policy for over 30 years. Recently it has received even more attention due to rising gas prices and concern over the environment and energy dependence. The model gives quantitative predictions for market fuel efficiency at various gas prices and taxes. The model makes contributions that are both methodological and policy based, and the two chapters of the dissertation focus on each in turn. The first chapter discusses the economic model of the U.S. automobile industry. The model allows firms to choose the fuel efficiency of their new vehicles, which allows me to predict fuel efficiency responses to policy and market conditions. These predictions were not possible with previous economic models which held fuel efficiency fixed. In the model, consumers care more about fuel efficiency when gas prices are high, and firms face a technological tradeoff between providing fuel efficiency and other quality. The level of the gas price, therefore, working through consumer demand, shifts firms' optimal locations along this technology frontier. Demand is nested logit, supply is differentiated products oligopoly, and data are from the U.S. automobile market from 1971-2007. In addition to endogenizing product choice, I also contribute to the modeling literature by relaxing restrictive identifying assumptions and obtaining more realistic estimates of fuel efficiency preference. The model predicts sales declines and compositions from the summer of 2008 with reasonable success. The second chapter discusses two counterfactual policy scenarios: maintained summer 2008 gas prices, and achieving 35 mpg (miles per gallon). At 3.43 per gallon (the summer 2008 price, 23% above 2007), the model predicts

  19. Ubiquitin binding by the CUE domain promotes endosomal localization of the Rab5 GEF Vps9

    PubMed Central

    Shideler, Tess; Nickerson, Daniel P.; Merz, Alexey J.; Odorizzi, Greg

    2015-01-01

    Vps9 and Muk1 are guanine nucleotide exchange factors (GEFs) in Saccharomyces cerevisiae that regulate membrane trafficking in the endolysosomal pathway by activating Rab5 GTPases. We show that Vps9 is the primary Rab5 GEF required for biogenesis of late endosomal multivesicular bodies (MVBs). However, only Vps9 (but not Muk1) is required for the formation of aberrant class E compartments that arise upon dysfunction of endosomal sorting complexes required for transport (ESCRTs). ESCRT dysfunction causes ubiquitinated transmembrane proteins to accumulate at endosomes, and we demonstrate that endosomal recruitment of Vps9 is promoted by its ubiquitin-binding CUE domain. Muk1 lacks ubiquitin-binding motifs, but its fusion to the Vps9 CUE domain allows Muk1 to rescue endosome morphology, cargo trafficking, and cellular stress-tolerance phenotypes that result from loss of Vps9 function. These results indicate that ubiquitin binding by the CUE domain promotes Vps9 function in endolysosomal membrane trafficking via promotion of localization. PMID:25673804

  20. Regulation and function of P-Rex family Rac-GEFs

    PubMed Central

    Welch, Heidi CE

    2015-01-01

    The P-Rex family are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. They are distinguished from other Rac-GEFs through their synergistic mode of activation by the lipid second messenger phosphatidyl inositol (3,4,5) trisphosphate and the Gβγ subunits of heterotrimeric G proteins, thus acting as coincidence detectors for phosphoinositide 3-kinase and G protein coupled receptor signaling. Work in genetically-modified mice has shown that P-Rex1 has physiological importance in the inflammatory response and the migration of melanoblasts during development, whereas P-Rex2 controls the dendrite morphology of cerebellar Purkinje neurons as well as glucose homeostasis in liver and adipose tissue. Deregulation of P-Rex1 and P-Rex2 expression occurs in many types of cancer, and P-Rex2 is frequently mutated in melanoma. Both GEFs promote tumor growth or metastasis. This review critically evaluates the P-Rex literature and tools available and highlights exciting recent developments and open questions. PMID:25961466

  1. The Role of Emerging Technologies in Improving Energy Efficiency:Examples from the Food Processing Industry

    SciTech Connect

    Lung, Robert Bruce; Masanet, Eric; McKane, Aimee

    2006-05-01

    For over 25 years, the U.S. DOE's Industrial Technologies Program (ITP) has championed the application of emerging technologies in industrial plants and monitored these technologies impacts on industrial energy consumption. The cumulative energy savings of more than 160 completed and tracked projects is estimated at approximately 3.99 quadrillion Btu (quad), representing a production cost savings of $20.4 billion. Properly documenting the impacts of such technologies is essential for assessing their effectiveness and for delivering insights about the optimal direction of future technology research. This paper analyzes the impacts that several emerging technologies have had in the food processing industry. The analysis documents energy savings, carbon emissions reductions and production improvements and assesses the market penetration and sector-wide savings potential. Case study data is presented demonstrating the successful implementation of these technologies. The paper's conclusion discusses the effects of these technologies and offers some projections of sector-wide impacts.

  2. Technology partnerships: Enhancing the competitiveness, efficiency, and environmental quality of American industry

    SciTech Connect

    1995-04-01

    An overview of the Department of Energy`s Office of Industrial Technologies and its private sector partnerships is presented. Commercial success stories and real-world benefits of the technology partnerships are discussed.

  3. Environmental regulation, productive efficiency and cost of pollution abatement: a case study of the sugar industry in India.

    PubMed

    Murty, M N; Kumar, Surender; Paul, Mahua

    2006-04-01

    In this paper the input distance function is estimated for the Indian Sugar industry under alternative assumptions of weak and strong disposability of bad outputs. The estimated distance function is used to make the estimates of environmental efficiency, Malmquist productivity index and shadow prices of pollutants. The technical efficiency measure estimated under the assumption of weak disposability of bad outputs is utilized to test the Porter hypothesis. Marginal costs of pollution abatement functions are estimated for different pollutants of water. Pollutant specific taxes are computed using the tax-standards method. PMID:16181728

  4. Ratepayer-funded energy-efficiency programs in a restructuredelectri city industry: Issues and options for regulators andlegislators

    SciTech Connect

    Eto, Joseph; Goldman, Charles; Nadel, Stephen

    1998-05-01

    Electric industry restructuring requires state regulators and legislators to re-examine the purposes served by and the continuing need for ratepayer-funded energy-efficiency programs, as well as the mechanisms to collect funds for these programs and the institutions appropriate to administer them. This paper offers background to these issues and a series of recommendations based on analysis of recent state experiences. Our recommendations are summarized.

  5. Liposome reconstitution and modulation of recombinant prenylated human Rac1 by GEFs, GDI1 and Pak1.

    PubMed

    Zhang, Si-Cai; Gremer, Lothar; Heise, Henrike; Janning, Petra; Shymanets, Aliaksei; Cirstea, Ion C; Krause, Eberhard; Nürnberg, Bernd; Ahmadian, Mohammad Reza

    2014-01-01

    Small Rho GTPases are well known to regulate a variety of cellular processes by acting as molecular switches. The regulatory function of Rho GTPases is critically dependent on their posttranslational modification at the carboxyl terminus by isoprenylation and association with proper cellular membranes. Despite numerous studies, the mechanisms of recycling and functional integration of Rho GTPases at the biological membranes are largely unclear. In this study, prenylated human Rac1, a prominent member of the Rho family, was purified in large amount from baculovirus-infected Spodoptera frugiperda insect cells using a systematic detergent screening. In contrast to non-prenylated human Rac1 purified from Escherichia coli, prenylated Rac1 from insect cells was able to associate with synthetic liposomes and to bind Rho-specific guanine nucleotide dissociation inhibitor 1 (GDI1). Subsequent liposome reconstitution experiments revealed that GDI1 efficiently extracts Rac1 from liposomes preferentially in the inactive GDP-bound state. The extraction was prevented when Rac1 was activated to its GTP-bound state by Rac-specific guanine nucleotide exchange factors (GEFs), such as Vav2, Dbl, Tiam1, P-Rex1 and TrioN, and bound by the downstream effector Pak1. We found that dissociation of Rac1-GDP from its complex with GDI1 strongly correlated with two distinct activities of especially Dbl and Tiam1, including liposome association and the GDP/GTP exchange. Taken together, our results provided first detailed insights into the advantages of the in vitro liposome-based reconstitution system to study both the integration of the signal transducing protein complexes and the mechanisms of regulation and signaling of small GTPases at biological membranes. PMID:25014207

  6. Liposome Reconstitution and Modulation of Recombinant Prenylated Human Rac1 by GEFs, GDI1 and Pak1

    PubMed Central

    Zhang, Si-Cai; Gremer, Lothar; Heise, Henrike; Janning, Petra; Shymanets, Aliaksei; Cirstea, Ion C.; Krause, Eberhard; Nürnberg, Bernd; Ahmadian, Mohammad Reza

    2014-01-01

    Small Rho GTPases are well known to regulate a variety of cellular processes by acting as molecular switches. The regulatory function of Rho GTPases is critically dependent on their posttranslational modification at the carboxyl terminus by isoprenylation and association with proper cellular membranes. Despite numerous studies, the mechanisms of recycling and functional integration of Rho GTPases at the biological membranes are largely unclear. In this study, prenylated human Rac1, a prominent member of the Rho family, was purified in large amount from baculovirus-infected Spodoptera frugiperda insect cells using a systematic detergent screening. In contrast to non-prenylated human Rac1 purified from Escherichia coli, prenylated Rac1 from insect cells was able to associate with synthetic liposomes and to bind Rho-specific guanine nucleotide dissociation inhibitor 1 (GDI1). Subsequent liposome reconstitution experiments revealed that GDI1 efficiently extracts Rac1 from liposomes preferentially in the inactive GDP-bound state. The extraction was prevented when Rac1 was activated to its GTP-bound state by Rac-specific guanine nucleotide exchange factors (GEFs), such as Vav2, Dbl, Tiam1, P-Rex1 and TrioN, and bound by the downstream effector Pak1. We found that dissociation of Rac1-GDP from its complex with GDI1 strongly correlated with two distinct activities of especially Dbl and Tiam1, including liposome association and the GDP/GTP exchange. Taken together, our results provided first detailed insights into the advantages of the in vitro liposome-based reconstitution system to study both the integration of the signal transducing protein complexes and the mechanisms of regulation and signaling of small GTPases at biological membranes. PMID:25014207

  7. Cdk5-mediated phosphorylation of RapGEF2 controls neuronal migration in the developing cerebral cortex.

    PubMed

    Ye, Tao; Ip, Jacque P K; Fu, Amy K Y; Ip, Nancy Y

    2014-01-01

    During cerebral cortex development, pyramidal neurons migrate through the intermediate zone and integrate into the cortical plate. These neurons undergo the multipolar-bipolar transition to initiate radial migration. While perturbation of this polarity acquisition leads to cortical malformations, how this process is initiated and regulated is largely unknown. Here we report that the specific upregulation of the Rap1 guanine nucleotide exchange factor, RapGEF2, in migrating neurons corresponds to the timing of this polarity transition. In utero electroporation and live-imaging studies reveal that RapGEF2 acts on the multipolar-bipolar transition during neuronal migration via a Rap1/N-cadherin pathway. Importantly, activation of RapGEF2 is controlled via phosphorylation by a serine/threonine kinase Cdk5, whose activity is largely restricted to the radial migration zone. Thus, the specific expression and Cdk5-dependent phosphorylation of RapGEF2 during multipolar-bipolar transition within the intermediate zone are essential for proper neuronal migration and wiring of the cerebral cortex. PMID:25189171

  8. Assessing the relative efficiency of water companies in the English and Welsh water industry: a metafrontier approach.

    PubMed

    Molinos-Senante, María; Maziotis, Alexandros; Sala-Garrido, Ramon

    2015-11-01

    The assessment of relative efficiency of water companies is essential for managers and authorities. This is evident in the UK water sector where there are companies with different services such as water and sewerage companies (WaSCs) and water-only companies (WoCs). Therefore, it is a critical limitation to estimate a common production frontier for both types of companies, as it might lead to biased efficiency estimates. In this paper, a robust and reliable methodology, the metafrontier model, is applied to compare the efficiency of water companies providing different services. The results illustrate the superior performance of WaSCs compared to WoCs. It also confirms the presence of economies of scope in the UK water industry. The methodology and results of this study are of great interest for both regulators and water utility managers to make informed decisions. PMID:26122569

  9. Synthesizing Equivalence Indices for the Comparative Evaluation of Technoeconomic Efficiency of Industrial Processes at the Design/Re-engineering Level

    NASA Astrophysics Data System (ADS)

    Fotilas, P.; Batzias, A. F.

    2007-12-01

    The equivalence indices synthesized for the comparative evaluation of technoeconomic efficiency of industrial processes are of critical importance since they serve as both, (i) positive/analytic descriptors of the physicochemical nature of the process and (ii) measures of effectiveness, especially helpful for investigated competitiveness in the industrial/energy/environmental sector of the economy. In the present work, a new algorithmic procedure has been developed, which initially standardizes a real industrial process, then analyzes it as a compromise of two ideal processes, and finally synthesizes the index that can represent/reconstruct the real process as a result of the trade-off between the two ideal processes taking as parental prototypes. The same procedure makes fuzzy multicriteria ranking within a set of pre-selected industrial processes for two reasons: (a) to analyze the process most representative of the production/treatment under consideration, (b) to use the `second best' alternative as a dialectic pole in absence of the two ideal processes mentioned above. An implantation of this procedure is presented, concerning a facility of biological wastewater treatment with six alternatives: activated sludge through (i) continuous-flow incompletely-stirred tank reactors in series, (ii) a plug flow reactor with dispersion, (iii) an oxidation ditch, and biological processing through (iv) a trickling filter, (v) rotating contactors, (vi) shallow ponds. The criteria used for fuzzy (to count for uncertainty) ranking are capital cost, operating cost, environmental friendliness, reliability, flexibility, extendibility. Two complementary indices were synthesized for the (ii)-alternative ranked first and their quantitative expressions were derived, covering a variety of kinetic models as well as recycle/bypass conditions. Finally, analysis of estimating the optimal values of these indices at maximum technoeconomic efficiency is presented and the implications

  10. 78 FR 26544 - Energy Efficiency Program for Commercial and Industrial Equipment: Public Meeting and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-07

    ... commercial and industrial fan and blower equipment that published on February 1, 2013, (78 FR 7306) is... amended (76 FR 37628, June 28, 2011). As part of its further consideration of this determination, DOE is... availability of the framework document to consider such standards (78 FR 7306, Feb. 1, 2013). The...

  11. 75 FR 71596 - Energy Efficiency Program for Certain Commercial and Industrial Equipment: Test Procedures for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-24

    ... commercial freezers specifically designed for ice cream application. 71 FR 71357. In addition, DOE adopted... equipment classes on January 9, 2009. 74 FR 1091. Manufacturers of covered equipment, including commercial...-2004) for measuring refrigerated compartment volume. 71 FR 71370. These industry standards...

  12. An efficient process for producing economical and eco-friendly cotton textile composites for mobile industry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The mobile industry comprised of airplanes, automotives, and ships uses enormous quantities of various types of textiles. Just a few decades ago, most of these textile products and composites were made with woven or knitted fabrics that were mostly made with the then only available natural fibers, i...

  13. Drosophila RhoGEF4 encodes a novel RhoA-specific guanine exchange factor that is highly expressed in the embryonic central nervous system.

    PubMed

    Nahm, Minyeop; Lee, Mihye; Baek, Seung-Hak; Yoon, Jin-Ho; Kim, Hong-Hee; Lee, Zang Hee; Lee, Seungbok

    2006-12-15

    Rho family small GTPases act as molecular switches that regulate neuronal morphogenesis, including axon growth and guidance, dendritic spine formation, and synapse formation. These proteins are positively regulated by guanine nucleotide exchange factors (GEFs) of the Dbl family. This study describes the identification and characterization of Drosophila RhoGEF4 (DRhoGEF4), a novel Dbl family protein that is specifically expressed in the central nervous system during Drosophila embryogenesis. The predicted amino acid sequence of DRhoGEF4 contains a Dbl homology (DH) domain and an adjacent C-terminal pleckstrin homology (PH) domain, which are most closely related to those of mammalian frabins. In this study, the DH-PH motif is shown to enhance the dissociation of GDP from either RhoA or Rac1 but not from Cdc42 in vitro. In addition, p21-binding domain pull-down assays demonstrate that DRhoGEF4 activates RhoA, but neither Rac1 nor Cdc42 in HEK293 cells. Finally, overexpression of DRhoGEF4 is able to induce assembly of stress fibers in cultured NIH3T3 cells. Taken together, these findings suggest that DRhoGEF4 may participate in cytoskeleton-related cellular events by specifically activating RhoA in neuronal morphogenesis. PMID:17011730

  14. The Putative Exchange Factor Gef3p Interacts with Rho3p GTPase and the Septin Ring during Cytokinesis in Fission Yeast*

    PubMed Central

    Muñoz, Sofía; Manjón, Elvira; Sánchez, Yolanda

    2014-01-01

    The small GTP-binding proteins of the Rho family and its regulatory proteins play a central role in cytokinetic actomyosin ring assembly and cytokinesis. Here we show that the fission yeast guanine nucleotide exchange factor Gef3p interacts with Rho3p at the division site. Gef3p contains a putative DH homology domain and a BAR/IMD-like domain. The protein localized to the division site late in mitosis, where it formed a ring that did not constrict with actomyosin ring (cytokinetic actomyosin ring) invagination; instead, it split into a double ring that resembled the septin ring. Gef3p co-localized with septins and Mid2p and required septins and Mid2p for its localization. Gef3p interacts physically with the GTP-bound form of Rho3p. Although Gef3p is not essential for cell separation, the simultaneous disruption of gef3+ and Rho3p-interacting proteins, such as Sec8p, an exocyst component, Apm1p, a subunit of the clathrin adaptor complex or For3p, an actin-polymerizing protein, yielded cells with strong defects in septation and polarity respectively. Our results suggest that interactions between septins and Rho-GEFs provide a new targeting mechanism for GTPases in cytokinesis, in this case probably contributing to Rho3p function in vesicle tethering and vesicle trafficking in the later steps of cell separation. PMID:24947517

  15. Efficiency, equity and the environment: Institutional challenges in the restructuring of the electric power industry

    SciTech Connect

    Haeri, M.H.

    1998-07-01

    In the electric power industry, fundamental changes are underway in Europe, America, Australia, New Zealand and, more recently, in Asia. Rooted in increased deregulation and competition, these changes are likely to radically alter the structure of the industry. Liberalization of electric power markets in the United Kingdom is, for the most part, complete. The generation market in the United States began opening to competition following the 1987 Public Utility Regulatory Policies Act (PURPA). The Energy Policy Act of 1992 set the stage for a much more dramatic change in the industry. The most far-reaching provision of the Act was its electricity title, which opened access to the electric transmission grid. With legal barriers now removed, the traditionally sheltered US electric utility market is becoming increasingly open to entry and competition. A number of important legislative, regulatory and governmental policy initiatives are underway in the Philippines that will have a profound effect on the electric power industry. In Thailand, the National Energy Planning Organization (NEPO) has undertaken a thorough investigation of industry restructuring. This paper summarizes recent international developments in the deregulation and liberalization of electricity markets in the U.K., U.S., Australia, and New Zealand. It focuses on the relevance of these experiences to development underway in the Philippines and Thailand, and presents alternative possible structures likely to emerge in these countries, drawing heavily on the authors' recent experiences in Thailand and the Philippines. The impact of these changes on the business environment for power generation and marketing will be discussed in detail, as will the opportunities these changes create for investment among private power producers.

  16. Industrial applications using BASF eco-efficiency analysis: perspectives on green engineering principles.

    PubMed

    Shonnard, David R; Kicherer, Andreas; Saling, Peter

    2003-12-01

    Life without chemicals would be inconceivable, but the potential risks and impacts to the environment associated with chemical production and chemical products are viewed critically. Eco-efficiency analysis considers the economic and life cycle environmental effects of a product or process, giving these equal weighting. The major elements of the environmental assessment include primary energy use, raw materials utilization, emissions to all media, toxicity, safety risk, and land use. The relevance of each environmental category and also for the economic versus the environmental impacts is evaluated using national emissions and economic data. The eco-efficiency analysis method of BASF is briefly presented, and results from three applications to chemical processes and products are summarized. Through these applications, the eco-efficiency analyses mostly confirm the 12 Principles listed in Anastas and Zimmerman (Environ. Sci. Technol. 2003, 37(5), 94A), with the exception that, in one application, production systems based on bio-based feedstocks were not the most eco-efficient as compared to those based on fossil resources. Over 180 eco-efficiency analyses have been conducted at BASF, and their results have been used to support strategic decision-making, marketing, research and development, and communication with external parties. Eco-efficiency analysis, as one important strategy and success factor in sustainable development, will continue to be a very strong operational tool at BASF. PMID:14700318

  17. Open Ocean Assessments for Management in the GEF Transboundary Waters Assessment Project (TWAP)

    NASA Astrophysics Data System (ADS)

    Fischer, A. S.; Alverson, K. D.

    2010-12-01

    A methodology for a thematic and scientifically-credible assessment of Open Ocean waters as a part of the Global Environment Facility (GEF) Transboundary Waters Assessment Project (TWAP) has been developed in the last 18 months by the Intergovernmental Oceanographic Commission of UNESCO, and is presented for feedback and comment. While developed to help the GEF International Waters focal area target investment to manage looming environmental threats in interlinked freshwater and marine systems (a very focused decision support system), the assessment methodology could contribute to other assessment and management efforts in the UN system and elsewhere. Building on a conceptual framework that describes the relationships between human systems and open ocean natural systems, and on mapping of the human impact on the marine environment, the assessment will evaluate and make projections on a thematic basis, identifying key metrics, indices, and indicators. These themes will include the threats on key ecosystem services of climate change through sea level rise, changed stratification, warming, and ocean acidification; vulnerabilities of ecosystems, habitats, and living marine resources; the impact and sustainability of fisheries; and pollution. Global-level governance arrangements will also be evaluated, with an eye to identifying scope for improved global-level management. The assessment will build on sustained ocean observing systems, model projections, and an assessment of scientific literature, as well as tools for combining knowledge to support identification of priority concerns and in developing scenarios for management. It will include an assessment of key research and observing needs as one way to deal with the scientific uncertainty inherent in such an exercise, and to better link policy and science agendas.

  18. Extracellular Superoxide Dismutase Regulates the Expression of Small GTPase Regulatory Proteins GEFs, GAPs, and GDI

    PubMed Central

    Laukkanen, Mikko O.; Cammarota, Francesca; Esposito, Tiziana; Salvatore, Marco; Castellone, Maria D.

    2015-01-01

    Extracellular superoxide dismutase (SOD3), which catalyzes the dismutation of superoxide anions to hydrogen peroxide at the cell membranes, regulates the cellular growth in a dose-dependent manner. This enzyme induces primary cell proliferation and immortalization at low expression levels whereas it activates cancer barrier signaling through the p53-p21 pathway at high expression levels, causing growth arrest, senescence, and apoptosis. Because previous reports suggested that the SOD3–induced reduction in the rates of cellular growth and migration also occurred in the absence of functional p53 signaling, in the current study we investigated the SOD3-induced growth-suppressive mechanisms in anaplastic thyroid cancer cells. Based on our data, the robust over-expression of SOD3 increased the level of phosphorylation of the EGFR, ERBB2, RYK, ALK, FLT3, and EPHA10 receptor tyrosine kinases with the consequent downstream activation of the SRC, FYN, YES, HCK, and LYN kinases. However, pull-down experiments focusing on the small GTPase RAS, RAC, CDC42, and RHO revealed a reduced level of growth and migration signal transduction, such as the lack of stimulation of the mitogen pathway, in the SOD3 over-expressing cells, which was confirmed by MEK1/2 and ERK1/2 Western blotting analysis. Interestingly, the mRNA expression analyses indicated that SOD3 regulated the expression of guanine nucleotide-exchange factors (RHO GEF16, RAL GEF RGL1), GTPase-activating proteins (ARFGAP ADAP2, RAS GAP RASAL1, RGS4), and a Rho guanine nucleotide-disassociation inhibitor (RHO GDI 2) in a dose dependent manner, thus controlling signaling through the small G protein GTPases. Therefore, our current data may suggest the occurrence of dose-dependent SOD3–driven control of the GTP loading of small G proteins indicating a novel growth regulatory mechanism of this enzyme. PMID:25751262

  19. Extracellular superoxide dismutase regulates the expression of small gtpase regulatory proteins GEFs, GAPs, and GDI.

    PubMed

    Laukkanen, Mikko O; Cammarota, Francesca; Esposito, Tiziana; Salvatore, Marco; Castellone, Maria D

    2015-01-01

    Extracellular superoxide dismutase (SOD3), which catalyzes the dismutation of superoxide anions to hydrogen peroxide at the cell membranes, regulates the cellular growth in a dose-dependent manner. This enzyme induces primary cell proliferation and immortalization at low expression levels whereas it activates cancer barrier signaling through the p53-p21 pathway at high expression levels, causing growth arrest, senescence, and apoptosis. Because previous reports suggested that the SOD3-induced reduction in the rates of cellular growth and migration also occurred in the absence of functional p53 signaling, in the current study we investigated the SOD3-induced growth-suppressive mechanisms in anaplastic thyroid cancer cells. Based on our data, the robust over-expression of SOD3 increased the level of phosphorylation of the EGFR, ERBB2, RYK, ALK, FLT3, and EPHA10 receptor tyrosine kinases with the consequent downstream activation of the SRC, FYN, YES, HCK, and LYN kinases. However, pull-down experiments focusing on the small GTPase RAS, RAC, CDC42, and RHO revealed a reduced level of growth and migration signal transduction, such as the lack of stimulation of the mitogen pathway, in the SOD3 over-expressing cells, which was confirmed by MEK1/2 and ERK1/2 Western blotting analysis. Interestingly, the mRNA expression analyses indicated that SOD3 regulated the expression of guanine nucleotide-exchange factors (RHO GEF16, RAL GEF RGL1), GTPase-activating proteins (ARFGAP ADAP2, RAS GAP RASAL1, RGS4), and a Rho guanine nucleotide-disassociation inhibitor (RHO GDI 2) in a dose dependent manner, thus controlling signaling through the small G protein GTPases. Therefore, our current data may suggest the occurrence of dose-dependent SOD3-driven control of the GTP loading of small G proteins indicating a novel growth regulatory mechanism of this enzyme. PMID:25751262

  20. Analysis of the Efficiency of the U.S. Ethanol Industry 2007

    SciTech Connect

    Wu, May

    2008-03-27

    In 2007, the Renewable Fuels Association (RFA) conducted a survey of US ethanol production plants to provide an assessment of the current US ethanol industry. The survey covers plant operations in both corn dry mills and wet mills. In particular, it includes plant type, ownership structure, capacity, feedstocks, production volumes, coproducts, process fuel and electricity usage, water consumption, and products transportation and distribution. This report includes a summary and analysis of these results.

  1. Efficiency of compost in the removal of heavy metals from the industrial wastewater

    NASA Astrophysics Data System (ADS)

    Kocasoy, Günay; Güvener, Zeynep

    2009-03-01

    Authorities have been applying very strict regulations for the treatment of industrial wastewater recently because of the threatening level of the environmental pollution faced. Industrial wastewater containing heavy metals is a threat to the public health because of the accumulation of the heavy metals in the aquatic life which is transferred to human bodies through the food chain. Therefore, recently, researchers have been oriented toward the practical use of adsorbents for the treatment of wastewater polluted by heavy metals. The aim of this research was to determine the retention capacity of compost for copper, zinc, nickel and chromium. For this purpose, experiments in batch-mixing reactors with initial metal concentrations ranging from 100 to 1,000 mg/l were carried. It was also observed that compost could repeatedly be used in metal sorption processes. The experiments conducted indicated that compost has high retention capacities for copper, zinc and nickel, but not for chromium. Thus, compost has been approved as a potential sorbent for copper, zinc and nickel and may find place in industrial applications. Thus, solid waste which is another source of significant environmental pollution will be reduced by being converted into a beneficial product compost.

  2. Towards The Industrialization Of Concentrator Solar Cells With Efficiencies Above 40%

    NASA Astrophysics Data System (ADS)

    Guter, W.; Meusel, M.; Köstler, W.; Kern, R.; Siefer, G.; Kellenbenz, R.; Dimroth, F.

    2010-10-01

    III-V Multi-junction solar cells provide today's highest photovoltaic conversion efficiencies and have entered the terrestrial market giving new drive to the concept of concentrator photovoltaics (CPV). Together with Fraunhofer ISE, AZUR SPACE has carried out intensive research regarding device architectures, material studies and processing. Continuous improvement of production processes at AZUR SPACE and the incorporation of a high bandgap top cell have recently led to concentrator cells with an average efficiency of 39.1% (500×AM1.5d). The target for this structure is to achieve close to 40% efficiency after further optimization. Metamorphic cell structures will increase efficiencies to values above 40%. This paper summarizes the current production status of the 3C35 solar cell with efficiencies above 35% as well as the status of the 3C38 design with a lean production process and an average efficienciy of 38%. Furthermore the development of the 3C40 cell structure with high-bandgap top cell is introduced and first results on the transfer of the metamorphic cell design from Fraunhofer ISE to AZUR SPACE are presented.

  3. Future efficiency growth - a historic crossroads for the gas turbine industry

    SciTech Connect

    Touchton, G.; Cohn, A.

    1996-12-31

    In the 1980s, the efficiencies of the best gas turbine/steam turbine combined-cycle plants were in the 47-50% (LHV) range. Now there are operating plants with efficiencies of 55%, and plants being installed or announced are projected to achieve efficiencies in the 58-60% range. The major determinant of plant efficiency gain has been the improvement of gas turbine components-primarily the expander turbine and compressor. Firing temperatures have risen from under 2000F (1100C) to over 2600F (1430C), while pressure ratios-which were about 10:1-have risen to as high as 30:1. However, progress along this road is becoming ever more difficult, especially because of the necessity of meeting more stringent NOx emission requirements. In a historic shift, manufacturers are turning toward the technology path less traveled-more novel and complex cycles. The latest ABB gas turbine series, the GT24 and GT26, utilize high temperature reheat combustion, the GE 7H and 9H utilize closed-circuit steam cooling, and the CHAT cycle utilizes humidification. This paper shows that by properly combining these techniques, plants can achieve significant further efficiency improvement while maintaining NOx emissions at present levels.

  4. A new approach to energy-efficient treatment of wastewater produced by the fish industry in Vietnam.

    PubMed

    Trautmann, N; Beier, M; Phan, L C; Rosenwinkel, K H

    2011-01-01

    Economic growth in Vietnam in the last few years has brought about an increasing demand for energy and has had a severe environmental impact. Fish processing is one of the fastest-growing industries that discharge organically-polluted wastewater. To counter these environmental problems, new technologies for energy-efficient treatment are needed. By coupling innovative nitrogen removal systems with anaerobic treatment processes, it is possible to realise such technologies. In the present project, a combined deammonification and anaerobic carbon removal system is presented. Special requirements to enable reliable treatment are discussed, taking industrial wastewater characteristics into consideration. To evaluate energetic efficiency, energy balance calculations based on data from a fish-processing factory are made. The determined specific energy consumption and production rates show that energy recovery is possible, even when COD and nitrogen removal efficiencies of over 90% are achieved. Depending on the pre-treatment employed, energy recovery rates ranging from 0.6 to 2.5 kWh/mt raw fish can be reached. PMID:22053486

  5. Asian success stories in promoting energy efficiency in industry and building

    SciTech Connect

    Yang, Ming

    1996-12-31

    This article describes the program of the International Institute for Energy Conservation (IIEC), which has offices in Washington, Bangkok, Santiago, and London, in addition to staff in a number of other countries. The mission of this private organization is to promote the efficient use of energy as a tool for sustainable development by supporting the development of policies, technologies, and practices. Its focus is on energy efficiency, transportation systems, and renewable energy sources. Examples of specific program activities in Thailand, China, Philippines, Malaysia, Indonesia and Singapore are discussed.

  6. Assessment of Energy Efficiency Improvement and CO2 Emission Reduction Potentials in the Cement Industry in China

    SciTech Connect

    Hasanbeigi, Ali; Morrow, William; Masanet, Eric; Sathaye, Jayant; Xu, Tengfang

    2012-06-15

    China’s annual cement production (i.e., 1,868 Mt) in 2010 accounted for nearly half of the world’s annual cement production in the same year. We identified and analyzed 23 energy efficiency technologies and measures applicable to the processes in the cement industry. The Conservation Supply Curve (CSC) used in this study is an analytical tool that captures both the engineering and the economic perspectives of energy conservation. Using a bottom-up electricity CSC model, the cumulative cost-effective electricity savings potential for the Chinese cement industry for 2010-2030 is estimated to be 251 TWh, and the total technical electricity saving potential is 279 TWh. The CO2 emissions reduction associated with cost-effective electricity savings is 144 Mt CO2 and the CO2 emission reduction associated with technical electricity saving potential is 161 Mt CO2. The fuel CSC model for the cement industry suggests cumulative cost-effective fuel savings potential of 4,326 PJ which is equivalent to the total technical potential with associated CO2 emission reductions of 406 Mt CO2. In addition, a sensitivity analysis with respect to the discount rate used is conducted to assess the effect of changes in this parameter on the results. We also developed a scenario in which instead of only implementing the international technologies in 2010-2030, we implement both international and Chinese domestic technologies during the analysis period and calculate the saving and cost of conserved energy accordingly. The result of this study gives a comprehensive and easy to understand perspective to the Chinese cement industry and policy makers about the energy efficiency potential and its associated cost.

  7. Energy Efficient Homes and Small Buildings. Vocational Education, Industrial Arts Curriculum Guide. Bulletin 1698.

    ERIC Educational Resources Information Center

    Louisiana State Dept. of Education, Baton Rouge. Div. of Vocational Education.

    This curriculum guide provides high school carpentry, construction, or drafting course teachers with material related to retrofitting a building for energy conservation. Section 1 discusses how design and construction methods affect energy use. Section 2 focuses on care and maintenance of energy efficient buildings. In addition to informative…

  8. 78 FR 72077 - Energy Efficiency Program for Industrial Equipment: Final Determination Classifying UL...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-02

    ... Small Electric Motors AGENCY: Office of Energy Efficiency and Renewable Energy, Department of Energy... contains energy conservation requirements for, among other things, electric motors and small electric... program for electric motors. See 77 FR 26608, 26629 (codifying provisions parallel to electric motors...

  9. 78 FR 57137 - Energy Efficiency Program for Industrial Equipment: Interim Determination Classifying UL...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-17

    ... program for electric motors. See 77 FR 26608, 26629 (codifying parallel provisions for small electric... Small Electric Motors AGENCY: Office of Energy Efficiency and Renewable Energy, Department of Energy... contains energy conservation requirements for, among other things, electric motors and small...

  10. 78 FR 17890 - Energy Efficiency Program for Commercial and Industrial Equipment: Public Meeting and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-25

    ... standards for packaged terminal air conditioners and packaged terminal heat pumps. 78 FR 12252. The document... Meeting and Availability of the Framework Document for Packaged Terminal Air Conditioners and Packaged Terminal Heat Pumps AGENCY: Office of Energy Efficiency and Renewable Energy, Department of Energy....

  11. 78 FR 14024 - Energy Efficiency Program for Commercial and Industrial Equipment: Public Meeting and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-04

    ... Federal Register on February 22, 2013 (78 FR 12252), concerning an announcement of a public meeting and... Equipment: Public Meeting and Availability of the Framework Document for Packaged Terminal Air Conditioners and Packaged Terminal Heat Pumps; Correction AGENCY: Office of Energy Efficiency and Renewable...

  12. Design of a reluctance motor with increased efficiency and power factor for widespread industrial application

    NASA Astrophysics Data System (ADS)

    Hansen-Goos, P.; Pieper, W.

    1981-09-01

    The motors were designed in the range of 0.6-4 kW with 4-pole windings. An increase of nominal power by approximately 100%, and an increase of quality factor by approximately 50% are reported. The efficiency is equal to asynchronous motors of the same IEC size.

  13. Policy Options for Encouraging Energy Efficiency Best Practices in Shandong Province's Cement Industry

    SciTech Connect

    Price, Lynn; Zhou, Nan; Lu, Hongyou; Sambeek, Emiel van; Yowargana, Ping; Shuang, Liu; Kejun, Jiang

    2012-07-12

    This research intends to explore possible design options for a sectoral approach in the cement sector in Shandong Province and to consider its respective advantages and disadvantages for future application. An effort has been made in this research to gather and analyze data that will provide a transparent and robust basis for development of a Business-As-Usual (BAU) scenario, maximum technology potential scenario, and ultimately a sector crediting baseline. Surveys among cement companies and discussions with stakeholders were also conducted in order to better understand the industry and local needs related to the sectoral approach.

  14. Bottom-up Representation of Industrial Energy Efficiency Technologies in Integrated Assessment Models for the Cement Sector

    SciTech Connect

    Sathaye, J.; Xu, T.; Galitsky, C.

    2010-08-15

    Adoption of efficient end-use technologies is one of the key measures for reducing greenhouse gas (GHG) emissions. How to effectively analyze and manage the costs associated with GHG reductions becomes extremely important for the industry and policy makers around the world. Energy-climate (EC) models are often used for analyzing the costs of reducing GHG emissions for various emission-reduction measures, because an accurate estimation of these costs is critical for identifying and choosing optimal emission reduction measures, and for developing related policy options to accelerate market adoption and technology implementation. However, accuracies of assessing of GHG-emission reduction costs by taking into account the adoption of energy efficiency technologies will depend on how well these end-use technologies are represented in integrated assessment models (IAM) and other energy-climate models.

  15. Condensing economizers for efficiency improvement and emissions control in industrial boilers

    SciTech Connect

    Butcher, T.A.; Litzke, W.L.; Schulze, K.; Bailey, R.

    1996-06-01

    Condensing economizers recover sensible and latent heat from boiler flue gas, leading to marked improvements in thermal efficiency. This paper summarizes the current commercial status and continuing development efforts with one type of condensing economizer. In this design Teflon{reg_sign} covered tubes and enclosure walls are used to handle the corrosive condensate. Flue gas flows around the tubes and feed water, being heated, flows through the inside. In addition to improving thermal efficiency, condensing economizers can also be used to reduce particulate emissions primarily by inertial impaction of particles on tube surfaces, water droplets, and added impactors. Collected particles are then removed with condensate. Water sprays directly on the tubes can be used to enhance particle capture. With coal-firing, tests have shown particle removal efficiencies as high as 98%. To enhance the emissions control potential of condensing economizer technology a two-stage economizer system concept has been developed. Two heat exchanger modules are used. The first is a downflow design and recovers primarily sensible heat from the flue gas. The second is upflow and recovers mostly latent heat. Condensate is collected in a transition plenum between the two stages. This configuration, termed the Integrated Flue Gas Treatment System, provides great flexibility for implementing emissions reduction strategies. Particulate emissions can be reduced without impacting sensible heat recovery by recirculating collected condensate to spray nozzles at the top of the second stage heat exchanger. In tests at BNL with heavy oil firing, particulate reductions over 90% and final emission rates on the order of.005 lb/MMBtu are achieved. Adding sorbents to the recirculated condensate reduces sulfur dioxide emissions and SO{sub 2} removal efficiencies over 95% are achieved. Also, condensing economizers show great potential for the removal of certain air toxics such as mercury and nickel.

  16. Sara Lee: Improved Compressed Air System Increases Efficiency and Saves Energy at an Industrial Bakery

    SciTech Connect

    Not Available

    2005-07-01

    This case study was prepared for the Industrial Technologies Program of the U.S. Department of Energy (DOE); it describes the energy and costs savings resulting from improving the compressed air system of a large Sara Lee bakery in Sacramento, California. The compressed air system supports many operations of the bread-making machines, and it had been performing poorly. A specialist from Draw Professional Services, a DOE Allied Partner, evaluated the system, and his suggestions included repairing a controller, fixing leaks, and replacing a compressor with a new one fitted with an energy-saving variable-speed drive. As a result, the bakery has reduced its energy use by 471,000 kilowatt-hours annually and is saving $50,000 per year in operating and maintenance costs.

  17. Revitalize the US silicon/ferrosilicon industry through energy-efficient technology. Part 1, Final report

    SciTech Connect

    Larson, H.R.; Welborn, J.H.

    1995-02-01

    It is concluded that silicon metal and ferrosilicon can be very effectively produced in a DC submerged arc furnace. Specific energy consumption factors measured were favorable to the technology. Significant energy savings over conventional AC practice are likely. Hollow electrode feeding of the furnace does not appear feasible. Electrode consumption was 0.144 lbs/lb so silicon while making metal, much of which occurred above the burden pile. Silicon loss to fume averaged 19.5% of the silicon charge. In this furnace, 50% FeSi was more difficult to produce than silicon metal, and the furnace could not be run with full burden; it was operated successfully about 3/4 full. In the silicon metal portion, the furnace was operated in a fully submerged mode for several 3-day test campaigns. The industry must seriously consider the identified benefits of DC plasma arc technology for retrofit or new added silicon capacity.

  18. Carbon disulfide-formic acid: an efficient desorbant for industrial solvents

    SciTech Connect

    Raulinaitis, I.

    1987-11-01

    When vapors of one solvent are collected on a charcoal tube, the desorbants suggested by NIOSH are adequate. When several solvents are collected at the same time, however, then a desorbant suggested for one solvent may mask some of the analytes or some of the analytes may be desorbed very poorly. An actual sample containing 13 solvents was brought to the laboratory for analysis. All solvents could be resolved on a 6-m 10% FFAP column, but some could not be desorbed efficiently with carbon disulfide; masking problems were encountered with other desorbants. Several solvents were tried; the most promising one was carbon disulfide saturated with formic acid.

  19. The Rho-guanine nucleotide exchange factor PDZ-RhoGEF governs susceptibility to diet-induced obesity and type 2 diabetes

    PubMed Central

    Chang, Ying-Ju; Pownall, Scott; Jensen, Thomas E; Mouaaz, Samar; Foltz, Warren; Zhou, Lily; Liadis, Nicole; Woo, Minna; Hao, Zhenyue; Dutt, Previn; Bilan, Philip J; Klip, Amira; Mak, Tak; Stambolic, Vuk

    2015-01-01

    Adipose tissue is crucial for the maintenance of energy and metabolic homeostasis and its deregulation can lead to obesity and type II diabetes (T2D). Using gene disruption in the mouse, we discovered a function for a RhoA-specific guanine nucleotide exchange factor PDZ-RhoGEF (Arhgef11) in white adipose tissue biology. While PDZ-RhoGEF was dispensable for a number of RhoA signaling-mediated processes in mouse embryonic fibroblasts, including stress fiber formation and cell migration, it's deletion led to a reduction in their proliferative potential. On a whole organism level, PDZ-RhoGEF deletion resulted in an acute increase in energy expenditure, selectively impaired early adipose tissue development and decreased adiposity in adults. PDZ-RhoGEF-deficient mice were protected from diet-induced obesity and T2D. Mechanistically, PDZ-RhoGEF enhanced insulin/IGF-1 signaling in adipose tissue by controlling ROCK-dependent phosphorylation of the insulin receptor substrate-1 (IRS-1). Our results demonstrate that PDZ-RhoGEF acts as a key determinant of mammalian metabolism and obesity-associated pathologies. DOI: http://dx.doi.org/10.7554/eLife.06011.001 PMID:26512886

  20. The Rho-guanine nucleotide exchange factor PDZ-RhoGEF governs susceptibility to diet-induced obesity and type 2 diabetes.

    PubMed

    Chang, Ying-Ju; Pownall, Scott; Jensen, Thomas E; Mouaaz, Samar; Foltz, Warren; Zhou, Lily; Liadis, Nicole; Woo, Minna; Hao, Zhenyue; Dutt, Previn; Bilan, Philip J; Klip, Amira; Mak, Tak; Stambolic, Vuk

    2015-01-01

    Adipose tissue is crucial for the maintenance of energy and metabolic homeostasis and its deregulation can lead to obesity and type II diabetes (T2D). Using gene disruption in the mouse, we discovered a function for a RhoA-specific guanine nucleotide exchange factor PDZ-RhoGEF (Arhgef11) in white adipose tissue biology. While PDZ-RhoGEF was dispensable for a number of RhoA signaling-mediated processes in mouse embryonic fibroblasts, including stress fiber formation and cell migration, it's deletion led to a reduction in their proliferative potential. On a whole organism level, PDZ-RhoGEF deletion resulted in an acute increase in energy expenditure, selectively impaired early adipose tissue development and decreased adiposity in adults. PDZ-RhoGEF-deficient mice were protected from diet-induced obesity and T2D. Mechanistically, PDZ-RhoGEF enhanced insulin/IGF-1 signaling in adipose tissue by controlling ROCK-dependent phosphorylation of the insulin receptor substrate-1 (IRS-1). Our results demonstrate that PDZ-RhoGEF acts as a key determinant of mammalian metabolism and obesity-associated pathologies. PMID:26512886

  1. The Rho Guanine Nucleotide Exchange Factor DRhoGEF2 Is a Genetic Modifier of the PI3K Pathway in Drosophila.

    PubMed

    Chang, Ying-Ju; Zhou, Lily; Binari, Richard; Manoukian, Armen; Mak, Tak; McNeill, Helen; Stambolic, Vuk

    2016-01-01

    The insulin/IGF-1 signaling pathway mediates various physiological processes associated with human health. Components of this pathway are highly conserved throughout eukaryotic evolution. In Drosophila, the PTEN ortholog and its mammalian counterpart downregulate insulin/IGF signaling by antagonizing the PI3-kinase function. From a dominant loss-of-function genetic screen, we discovered that mutations of a Dbl-family member, the guanine nucleotide exchange factor DRhoGEF2 (DRhoGEF22(l)04291), suppressed the PTEN-overexpression eye phenotype. dAkt/dPKB phosphorylation, a measure of PI3K signaling pathway activation, increased in the eye discs from the heterozygous DRhoGEF2 wandering third instar larvae. Overexpression of DRhoGEF2, and it's functional mammalian ortholog PDZ-RhoGEF (ArhGEF11), at various stages of eye development, resulted in both dPKB/Akt-dependent and -independent phenotypes, reflecting the complexity in the crosstalk between PI3K and Rho signaling in Drosophila. PMID:27015411

  2. The UNC-73/Trio RhoGEF-2 domain is required in separate isoforms for the regulation of pharynx pumping and normal neurotransmission in C. elegans.

    PubMed

    Steven, Robert; Zhang, Lijia; Culotti, Joseph; Pawson, Tony

    2005-09-01

    In both Caenorhabditis elegans and Drosophila, UNC-73/Trio functions in axon guidance by signaling through the Rac GTPase to regulate cytoskeletal rearrangements necessary for growth cone migrations. Here, we show that the complex C. elegans unc-73 gene encodes at least eight differentially expressed UNC-73 intracellular protein isoforms. Previously reported mutations affecting UNC-73 isoforms encoding the Rac-specific RhoGEF-1 domain cause uncoordinated movement, correlating with defects in axon guidance. Mutations in isoforms encoding the Rho-specific RhoGEF-2 domain, which we describe here, result in L1 stage larval lethality with no associated axon guidance defects. Isoform-specific rescue experiments reveal separate functions for the various RhoGEF-2-containing UNC-73 isoforms, which would not likely be discovered by conventional genetic screening. UNC-73 D1 and D2 appear to function redundantly in pharynx muscle to regulate the rate and strength of pharynx pumping, and in the HSN neurons and vulval muscles to control egg laying. Isoforms C1, C2, E, and F act redundantly within the nervous system to regulate the speed of locomotion. The multiple UNC-73 isoforms containing Rac- and Rho-specific RhoGEF domains therefore have distinct physiological functions. In addition to its previously identified role involving RhoGEF-1 in migrating cells and growth cones, our data indicate that UNC-73 signals through RhoGEF-2 to regulate pharynx and vulva musculature and to modulate synaptic neurotransmission. PMID:16140983

  3. The Rho Guanine Nucleotide Exchange Factor DRhoGEF2 Is a Genetic Modifier of the PI3K Pathway in Drosophila

    PubMed Central

    Chang, Ying-Ju; Zhou, Lily; Binari, Richard; Manoukian, Armen; Mak, Tak; McNeill, Helen; Stambolic, Vuk

    2016-01-01

    The insulin/IGF-1 signaling pathway mediates various physiological processes associated with human health. Components of this pathway are highly conserved throughout eukaryotic evolution. In Drosophila, the PTEN ortholog and its mammalian counterpart downregulate insulin/IGF signaling by antagonizing the PI3-kinase function. From a dominant loss-of-function genetic screen, we discovered that mutations of a Dbl-family member, the guanine nucleotide exchange factor DRhoGEF2 (DRhoGEF22(l)04291), suppressed the PTEN-overexpression eye phenotype. dAkt/dPKB phosphorylation, a measure of PI3K signaling pathway activation, increased in the eye discs from the heterozygous DRhoGEF2 wandering third instar larvae. Overexpression of DRhoGEF2, and it’s functional mammalian ortholog PDZ-RhoGEF (ArhGEF11), at various stages of eye development, resulted in both dPKB/Akt-dependent and -independent phenotypes, reflecting the complexity in the crosstalk between PI3K and Rho signaling in Drosophila. PMID:27015411

  4. Efficient industrial dye decolorization by Bacillus sp. VUS with its enzyme system.

    PubMed

    Dawkar, Vishal V; Jadhav, Umesh U; Tamboli, Dhawal P; Govindwar, Sanjay P

    2010-10-01

    This work presents role of different enzymes in decolorization of industrial dye Orange T4LL by Bacillus sp. VUS. Bacillus sp. strain VUS decolorized dye Orange T4LL, under static anoxic condition in 24 h. During decolorization of Orange T4LL a significant induction in the activities of lignin peroxidase, tyrosinase, and reductases (NADH-DCIP, azo, and riboflavin) was observed. The biodegradation was monitored by Ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, and high performance liquid chromatography. The final products 4-methyl-2-o-tolylazo-benzene-1,3-diamine and [3-(phenyl-hydrazono)-cyclohexa-1,4-dienyl]-methanol were characterized by gas chromatography-mass spectroscopy. Phytotoxicity, COD, and BOD revealed non-toxicity of degraded products. Phytotoxicity study demonstrated non-toxicity of the biodegraded products for crop plants with respect to Triticum aestivum and Sorghum bicolor. Bacillus sp. VUS with its enzyme system could be a useful tool for textile effluent treatment. PMID:20655588

  5. Dynamic olfactometry and GC-TOFMS to monitor the efficiency of an industrial biofilter.

    PubMed

    Gutiérrez, M C; Martín, M A; Pagans, E; Vera, L; García-Olmo, J; Chica, A F

    2015-04-15

    Biofiltration is the most widely used technique for eliminating odours in waste treatment plants. Volatile organic compounds (VOCs) are among the odorous compounds emitted by waste management plants, and serve as variables to measure odour emissions depending on the type of aeration process used. In this work, we assess the performance of an industrial-scale biofilter where composting is the main source of VOCs and odour emissions. Dynamic olfactometry is the sensorial technique used to determine odour concentration, while gas chromatography-time of flight-mass spectrometry (GC-TOFMS) is used to perform the chemical characterization. This work examines a total of 82 compounds belonging to 15 odorous families of VOCs, particularly mercaptans, sulphur-containing compounds, alcohols and terpenes, among others. Principal component analysis (PCA) is used to assess the influence of each of these families of VOCs on the total variance of the measure with regard to both the input and output flow of the biofilter. Finally, partial least-squares (PLS) regression is used to estimate the odour concentration in each of the samples taken at the inlet and outlet of the biofilter in each of the samples based on the chemical information provided by chromatographic analysis. The study shows that there is an adequate correlation (r=0.9751) between real and estimated odour concentrations, both of which are expressed in European odour units per cubic metre (ou(E)·m(-3)). PMID:25647372

  6. Anaerobic-aerobic sequencing bioreactors improve energy efficiency for treatment of personal care product industry wastes.

    PubMed

    Ahammad, S Z; Bereslawski, J L; Dolfing, J; Mota, C; Graham, D W

    2013-07-01

    Personal care product (PCP) industry liquid wastes contain shampoo residues, which are usually treated by aerobic activated sludge (AS). Unfortunately, AS is expensive for PCP wastes because of high aeration and energy demands, whereas potentially energy-positive anaerobic designs cannot meet effluent targets. Therefore, combined anaerobic-aerobic systems may be the best solution. Seven treatment systems were assessed in terms of energy and treatment performance for shampoo wastes, including one aerobic, three anaerobic (HUASB, AHR and AnCSTR) and three anaerobic-aerobic reactor designs. COD removals were highest in the HUASB-aerobic (87.9 ± 0.4%) and AHR-aerobic (86.8±0.5%) systems, which used 69.2% and 62.5% less energy than aerobic AS. However, actual methane production rates were low relative to theoretical in the UASB and AHR units (∼10% methane/COD removed) compared with the AnCSTR unit (∼70%). Anaerobic-aerobic sequence reactors show promise for treating shampoo wastes, but optimal designs depend upon whether methane production or COD removal is most important to operations. PMID:23639409

  7. Talaromyces atroroseus, a New Species Efficiently Producing Industrially Relevant Red Pigments

    PubMed Central

    Frisvad, Jens C.; Yilmaz, Neriman; Thrane, Ulf; Rasmussen, Kasper Bøwig; Houbraken, Jos; Samson, Robert A.

    2013-01-01

    Some species of Talaromyces secrete large amounts of red pigments. Literature has linked this character to species such as Talaromyces purpurogenus, T. albobiverticillius, T. marneffei, and T. minioluteus often under earlier Penicillium names. Isolates identified as T. purpurogenus have been reported to be interesting industrially and they can produce extracellular enzymes and red pigments, but they can also produce mycotoxins such as rubratoxin A and B and luteoskyrin. Production of mycotoxins limits the use of isolates of a particular species in biotechnology. Talaromyces atroroseus sp. nov., described in this study, produces the azaphilone biosynthetic families mitorubrins and Monascus pigments without any production of mycotoxins. Within the red pigment producing clade, T. atroroseus resolved in a distinct clade separate from all the other species in multigene phylogenies (ITS, β-tubulin and RPB1), which confirm its unique nature. Talaromyces atroroseus resembles T. purpurogenus and T. albobiverticillius in producing red diffusible pigments, but differs from the latter two species by the production of glauconic acid, purpuride and ZG–1494α and by the dull to dark green, thick walled ellipsoidal conidia produced. The type strain of Talaromyces atroroseus is CBS 133442 PMID:24367630

  8. A novel GABRG2 mutation, p.R136*, in a family with GEFS+ and extended phenotypes.

    PubMed

    Johnston, Ann J; Kang, Jing-Qiong; Shen, Wangzhen; Pickrell, William O; Cushion, Thomas D; Davies, Jeffrey S; Baer, Kristin; Mullins, Jonathan G L; Hammond, Carrie L; Chung, Seo-Kyung; Thomas, Rhys H; White, Cathy; Smith, Phil E M; Macdonald, Robert L; Rees, Mark I

    2014-04-01

    Genetic mutations in voltage-gated and ligand-gated ion channel genes have been identified in a small number of Mendelian families with genetic generalised epilepsies (GGEs). They are commonly associated with febrile seizures (FS), childhood absence epilepsy (CAE) and particularly with generalised or genetic epilepsy with febrile seizures plus (GEFS+). In clinical practice, despite efforts to categorise epilepsy and epilepsy families into syndromic diagnoses, many generalised epilepsies remain unclassified with a presumed genetic basis. During the systematic collection of epilepsy families, we assembled a cohort of families with evidence of GEFS+ and screened for variations in the γ2 subunit of the γ-aminobutyric acid (GABA) type A receptor gene (GABRG2). We detected a novel GABRG2(p.R136*) premature translation termination codon in one index-case from a two-generation nuclear family, presenting with an unclassified GGE, a borderline GEFS+ phenotype with learning difficulties and extended behavioural presentation. The GABRG2(p.R136*) mutation segregates with the febrile seizure component of this family's GGE and is absent in 190 healthy control samples. In vitro expression assays demonstrated that γ2(p.R136*) subunits were produced, but had reduced cell-surface and total expression. When γ2(p.R136*) subunits were co-expressed with α1 and β2 subunits in HEK 293T cells, GABA-evoked currents were reduced. Furthermore, γ2(p.R136*) subunits were highly-expressed in intracellular aggregations surrounding the nucleus and endoplasmic reticulum (ER), suggesting compromised receptor trafficking. A novel GABRG2(p.R136*) mutation extends the spectrum of GABRG2 mutations identified in GEFS+ and GGE phenotypes, causes GABAA receptor dysfunction, and represents a putative epilepsy mechanism. PMID:24407264

  9. P-Rex and Vav Rac-GEFs in platelets control leukocyte recruitment to sites of inflammation

    PubMed Central

    Pan, Dingxin; Amison, Richard T.; Riffo-Vasquez, Yanira; Spina, Domenico; Cleary, Simon J.; Wakelam, Michael J.; Page, Clive P.; Pitchford, Simon C.

    2015-01-01

    The small GTPase Rac is required for neutrophil recruitment during inflammation, but its guanine-nucleotide exchange factor (GEF) activators seem dispensable for this process, which led us to investigate the possibility of cooperation between Rac-GEF families. Thioglycollate-induced neutrophil recruitment into the peritoneum was more severely impaired in P-Rex1−/− Vav1−/− (P1V1) or P-Rex1−/− Vav3−/− (P1V3) mice than in P-Rex null or Vav null mice, suggesting cooperation between P-Rex and Vav Rac-GEFs in this process. Neutrophil transmigration and airway infiltration were all but lost in P1V1 and P1V3 mice during lipopolysaccharide (LPS)-induced pulmonary inflammation, with altered intercellular adhesion molecule 1-dependent slow neutrophil rolling and strongly reduced L- and E-selectin–dependent adhesion in airway postcapillary venules. Analysis of adhesion molecule expression, neutrophil adhesion, spreading, and migration suggested that these defects were only partially neutrophil-intrinsic and were not obviously involving vascular endothelial cells. Instead, P1V1 and P1V3 platelets recapitulated the impairment of LPS-induced intravascular neutrophil adhesion and recruitment, showing P-Rex and Vav expression in platelets to be crucial. Similarly, during ovalbumin-induced allergic inflammation, pulmonary recruitment of P1V1 and P1V3 eosinophils, monocytes, and lymphocytes was compromised in a platelet-dependent manner, and airway inflammation was essentially abolished, resulting in improved airway responsiveness. Therefore, platelet P-Rex and Vav family Rac-GEFs play important proinflammatory roles in leukocyte recruitment. PMID:25538043

  10. A Novel GABRG2 Mutation, p.R136*, in a family with GEFS+ and extended phenotypes

    PubMed Central

    Shen, Wangzhen; Pickrell, William O.; Cushion, Thomas D.; Davies, Jeffrey S.; Baer, Kristin; Mullins, Jonathan G.L.; Hammond, Carrie L.; Chung, Seo-Kyung; Thomas, Rhys H.; White, Cathy; Smith, Phil E.M.

    2014-01-01

    Genetic mutations in voltage-gated and ligand-gated ion channel genes have been identified in a small number of Mendelian families with genetic generalised epilepsies (GGEs). They are commonly associated with febrile seizures (FS), childhood absence epilepsy (CAE) and particularly with generalised or genetic epilepsy with febrile seizures plus (GEFS+). In clinical practice, despite efforts to categorise epilepsy and epilepsy families into syndromic diagnoses, many generalised epilepsies remain unclassified with a presumed genetic basis. During the systematic collection of epilepsy families, we assembled a cohort of families with evidence of GEFS+ and screened for variations in the γ2 subunit of the γ-aminobutyric acid (GABA) type A receptor gene (GABRG2). We detected a novel GABRG2(p.R136*) premature translation termination codon in one index-case from a two-generation nuclear family, presenting with an unclassified GGE, a borderline GEFS+ phenotype with learning difficulties and autism spectrum disorder (ASD). The GABRG2(p.R136*) mutation segregates with the febrile seizure component of this family's GGE and is absent in 190 healthy control samples. In vitro expression assays demonstrated that γ2(p.R136*) subunits were produced, but had reduced cell-surface and total expression. When γ2(p.R136*) subunits were co-expressed with α1 and β2 subunits in HEK 293T cells, GABA–evoked currents were reduced. Furthermore, γ2(p.R136*) subunits were highly-expressed in intracellular aggregations surrounding the nucleus and endoplasmic reticulum (ER), suggesting compromised receptor trafficking. A novel GABRG2(p.R136*) mutation extends the spectrum of GABRG2 mutations identified in GEFS+ and GGE phenotypes, causes GABAA receptor dysfunction, and represents a putative epilepsy mechanism. PMID:24407264

  11. Analysis of Parametric Effects on Efficiency of the Brown Stock Washer in Paper Industry Using MATLAB

    NASA Astrophysics Data System (ADS)

    Kumar, Deepak; Kumar, Vivek; Singh, V. P.

    2009-07-01

    In the present paper, the effects of cake thickness and time on the efficiency of brown stock washer of the paper mill are studied by using mathematical model of pulp washing for the species of sodium and lignin ions. The mechanism of the diffusion- dispersion washing of the bed of the pulp fibers is mathematically modeled by the basic material balance and adsorption isotherm is used to describe the equilibrium between the concentration of the solute in the liquor and concentration of the solute on the fibers. To study the parametric effect, numerical solutions of the axial domain of the system governed by partial differential equations (transport and isotherm equations) for different boundary conditions are obtained by the "pdepe" solver in MATLAB source code. The effects of both the parameters are shown by three dimensional graphical representation as well as concentration profiles.

  12. Structural Insights into Arl1-Mediated Targeting of the Arf-GEF BIG1 to the trans-Golgi.

    PubMed

    Galindo, Antonio; Soler, Nicolas; McLaughlin, Stephen H; Yu, Minmin; Williams, Roger L; Munro, Sean

    2016-07-19

    The GTPase Arf1 is the major regulator of vesicle traffic at both the cis- and trans-Golgi. Arf1 is activated at the cis-Golgi by the guanine nucleotide exchange factor (GEF) GBF1 and at the trans-Golgi by the related GEF BIG1 or its paralog, BIG2. The trans-Golgi-specific targeting of BIG1 and BIG2 depends on the Arf-like GTPase Arl1. We find that Arl1 binds to the dimerization and cyclophilin binding (DCB) domain in BIG1 and report a crystal structure of human Arl1 bound to this domain. Residues in the DCB domain that bind Arl1 are required for BIG1 to locate to the Golgi in vivo. DCB domain-binding residues in Arl1 have a distinct conformation from those in known Arl1-effector complexes, and this plasticity allows Arl1 to interact with different effectors of unrelated structure. The findings provide structural insight into how Arf1 GEFs, and hence active Arf1, achieve their correct subcellular distribution. PMID:27373159

  13. Hsc70 chaperone activity underlies Trio GEF function in axon growth and guidance induced by netrin-1

    PubMed Central

    DeGeer, Jonathan; Kaplan, Andrew; Mattar, Pierre; Morabito, Morgane; Stochaj, Ursula; Kennedy, Timothy E.; Debant, Anne; Cayouette, Michel; Fournier, Alyson E.

    2015-01-01

    During development, netrin-1 is both an attractive and repulsive axon guidance cue and mediates its attractive function through the receptor Deleted in Colorectal Cancer (DCC). The activation of Rho guanosine triphosphatases within the extending growth cone facilitates the dynamic reorganization of the cytoskeleton required to drive axon extension. The Rac1 guanine nucleotide exchange factor (GEF) Trio is essential for netrin-1–induced axon outgrowth and guidance. Here, we identify the molecular chaperone heat shock cognate protein 70 (Hsc70) as a novel Trio regulator. Hsc70 dynamically associated with the N-terminal region and Rac1 GEF domain of Trio. Whereas Hsc70 expression supported Trio-dependent Rac1 activation, adenosine triphosphatase–deficient Hsc70 (D10N) abrogated Trio Rac1 GEF activity and netrin-1–induced Rac1 activation. Hsc70 was required for netrin-1–mediated axon growth and attraction in vitro, whereas Hsc70 activity supported callosal projections and radial neuronal migration in the embryonic neocortex. These findings demonstrate that Hsc70 chaperone activity is required for Rac1 activation by Trio and this function underlies netrin-1/DCC-dependent axon outgrowth and guidance. PMID:26323693

  14. Efficient Phenol Removal of Raw Industrial Wastewater from Phenolic Resin Plants using Crosslinked β-Cyclodextrin Adsorbent

    NASA Astrophysics Data System (ADS)

    Yamasaki, Hirohito; Matsui, Hisako; Fukunaga, Kimitoshi

    A series of sorbents with mean diameters no greater than φ75μm was prepared by a crosslinking reaction with β-cyclodextrin (βCyD) and diisocyanates followed by ball-milling. The sorbent synthesized with hexamethylene diisocyanate (HDI) as a crosslinker showed better phenol removal efficiency than that with toluene 2,6-diisocyanate (TDI). The greatest removal efficiency was achieved with βCyD/HDI (1/8) sorbent which was obtained by the crosslinking of βCyD with HDI in a 1 : 8 molar ratio. The removal of the phenolic compounds from the raw industrial wastewater was almost instantaneous and the sorbents were efficiently regenerated by the elution of the adsorbed phenols with methanol. The βCyD/HDI (1/8) sorbent effectively removed phenol from phenolic wastewater containing 89 g/L phenol to concentrations as low as 117 mg/L, using five times accumulated batchwise treatments. Adsorption by the use of a liquid-solid fluidized bed was also carried out.

  15. A 400-kWe high-efficiency steam turbine for industrial cogeneration

    NASA Technical Reports Server (NTRS)

    Leibowitz, H. M.

    1982-01-01

    An advanced state-of-the-art steam turbine-generator developed to serve as the power conversion subsystem for the Department of Energy's Sandia National Laboratories' Solar Total-Energy Project (STEP) is described. The turbine-generator, which is designed to provide 400-kW of net electrical power, represents the largest turbine-generator built specifically for commercial solar-powered cogeneration. The controls for the turbine-generator incorporate a multiple, partial-arc entry to provide efficient off-design performance, as well as an extraction control scheme to permit extraction flow regulation while maintaining 110-spsig pressure. Normal turbine operation is achieved while synchronized to a local utility and in a stand-alone mode. In both cases, the turbine-generator features automatic load control as well as remote start-up and shutdown capability. Tests totaling 200 hours were conducted to confirm the integrity of the turbine's mechanical structure and control function. Performance tests resulted in a measured inlet throttle flow of 8,450 pounds per hour, which was near design conditions.

  16. A 400-kWe high-efficiency steam turbine for industrial cogeneration

    NASA Astrophysics Data System (ADS)

    Leibowitz, H. M.

    1982-07-01

    An advanced state-of-the-art steam turbine-generator developed to serve as the power conversion subsystem for the Department of Energy's Sandia National Laboratories' Solar Total-Energy Project (STEP) is described. The turbine-generator, which is designed to provide 400-kW of net electrical power, represents the largest turbine-generator built specifically for commercial solar-powered cogeneration. The controls for the turbine-generator incorporate a multiple, partial-arc entry to provide efficient off-design performance, as well as an extraction control scheme to permit extraction flow regulation while maintaining 110-spsig pressure. Normal turbine operation is achieved while synchronized to a local utility and in a stand-alone mode. In both cases, the turbine-generator features automatic load control as well as remote start-up and shutdown capability. Tests totaling 200 hours were conducted to confirm the integrity of the turbine's mechanical structure and control function. Performance tests resulted in a measured inlet throttle flow of 8,450 pounds per hour, which was near design conditions.

  17. Paxillin mediates stretch-induced Rho signaling and endothelial permeability via assembly of paxillin-p42/44MAPK-GEF-H1 complex.

    PubMed

    Gawlak, Grzegorz; Tian, Yufeng; O'Donnell, James J; Tian, Xinyong; Birukova, Anna A; Birukov, Konstantin G

    2014-07-01

    Suboptimal ventilator support or regional ventilation heterogeneity in inflamed lungs causes excessive tissue distension, which triggers stretch-induced pathological signaling and may lead to vascular leak and lung dysfunction. Focal adhesions (FAs) are cell-substrate adhesive complexes participating in cellular mechanotransduction and regulation of the Rho GTPase pathway. Stretch-induced Rho regulation remains poorly understood. We used human lung endothelial cells (ECs) exposed to pathological cyclic stretch (CS) at 18% distension to test the hypothesis that FA protein paxillin participates in CS-induced Rho activation by recruiting the Rho-specific guanine nucleotide exchange factor GEF-H1. CS induced phosphorylation of paxillin and activated p42/44-MAP kinase, Rho GTPase, and paxillin/GEF-H1/p42/44-MAPK association. CS caused nearly 2-fold increase in EC permeability, which was attenuated by paxillin knockdown. Expression of the paxillin-Y31/118F phosphorylation mutant decreased the CS-induced paxillin/GEF-H1 association (16.3 ± 4.1%), GEF-H1 activation (28.9 ± 9.2%), and EC permeability (28.7 ± 8.1%) but not CS-induced p42/44-MAPK activation. Inhibition of p42/44-MAPK suppressed CS-induced paxillin/GEF-H1 interactions (15.9 ± 7.9%), GEF-H1 activation (11.7 ± 4.3%), and disruption of EC monolayer. Expression of GEF-H1T678A lacking p42/44-MAPK phosphorylation site attenuated Rho activation (31.2±11.6%). We conclude that MAPK-dependent targeting of GEF-H1 to paxillin is involved in the regulation of CS-induced Rho signaling and EC permeability. This study proposes a novel concept of paxillin-GEF-H1-p42/44-MAPK module as a regulator of pathological mechanotransduction.-Gawlak, G., Tian, Y., O'Donnell, J. J., III, Tian, X., Birukova, A. A., Birukov, K. G. Paxillin mediates stretch-induced Rho signaling and endothelial permeability via assembly of paxillin-p42/44MAPK-GEF-H1 complex. PMID:24706358

  18. The Interplay between the Escherichia coli Rho Guanine Nucleotide Exchange Factor Effectors and the Mammalian RhoGEF Inhibitor EspH

    PubMed Central

    Wong, Alexander R. C.; Clements, Abigail; Raymond, Benoit; Crepin, Valerie F.; Frankel, Gad

    2012-01-01

    ABSTRACT Rho GTPases are important regulators of many cellular processes. Subversion of Rho GTPases is a common infection strategy employed by many important human pathogens. Enteropathogenic Escherichia coli and enterohemorrhagic Escherichia coli (EPEC and EHEC) translocate the effector EspH, which inactivates mammalian Rho guanine exchange factors (GEFs), as well as Map, EspT, and EspM2, which, by mimicking mammalian RhoGEFs, activate Rho GTPases. In this study we found that EspH induces focal adhesion disassembly, triggers cell detachment, activates caspase-3, and induces cytotoxicity. EspH-induced cell detachment and caspase-3 activation can be offset by EspT, EspM2, and the Salmonella Cdc42/Rac1 GEF effector SopE, which remain active in the presence of EspH. EPEC and EHEC therefore use a novel strategy of controlling Rho GTPase activity by translocating one effector to inactivate mammalian RhoGEFs, replacing them with bacterial RhoGEFs. This study also expands the functional range of bacterial RhoGEFs to include cell adhesion and survival. IMPORTANCE Many human pathogens use a type III secretion system to translocate effectors that can functionally be divided into signaling, disabling, and countervirulence effectors. Among the signaling effectors are those that activate Rho GTPases, which play a central role in coordinating actin dynamics. However, many pathogens also translocate effectors with antagonistic or counteractive functions. For example, Salmonella translocates SopE and SptP, which sequentially turn Rac1 and Cdc42 on and off. In this paper, we show that enteropathogenic E. coli translocates EspH, which inactivates mammalian RhoGEFs and triggers cytotoxicity and at the same time translocates the bacterial RhoGEFs EspM2 and EspT, which are insensitive to EspH, and so neutralizes EspH-induced focal adhesion disassembly, cell detachment, and caspase-3 activation. Our data point to an intriguing infection strategy in which EPEC and EHEC override cellular

  19. Projections of atmospheric nitrous oxide under scenarios of improved agriculture and industrial efficiencies, diet modification, and representative concentration pathways (RCPs)

    NASA Astrophysics Data System (ADS)

    Davidson, E. A.

    2011-12-01

    Atmospheric concentrations of nitrous oxide (N2O), now at about 325ppb, have been increasing since the Industrial Revolution, as livestock herds increased globally and as use of synthetic-N fertilizers increased after WWII. The agricultural sector produces 70-80% of anthropogenic N2O. Significantly reducing those emissions while also improving the diets of the growing global human population will be very challenging. Increases in atmospheric N2O since 1860 are consistent with emissions factors of 2.5% of annual fertilizer-N usage and 2.0% of annual manure-N production being converted to N2O. These factors include both direct and indirect emissions attributable to these sources. Here I present projections of N2O emissions for a variety of scenarios including: (1) FAO population/diet scenarios with no changes in emission factors; (2) per-capita protein consumption in the developed world declines to 1980 levels by 2030 and only half of that is obtained from animal products, thus cutting global manure production by about 20%; (3) improvements in N-use efficiency and manure management reduce the emission factors by 50% by 2050; (4) same as 3 but industrial and transportation emissions are similarly reduced by 50% by 2050; and (5) all mitigations together. These projections are then compared to the four representative concentration pathways (RCPs) developed for the IPCC-AR5. With no further mitigation, the projections are consistent with RCP8.5, with atmospheric N2O at 368 ppb in 2050. RCP8.5 is a reasonable representation of N2O concentrations with growing agricultural production to feed a growing and better-nourished population, without improvements in agricultural efficiencies or changes in developed world diets. Major reductions in per-capita meat consumption in the developed world reduce projected 2050 N2O to 256 ppb, which is in line with RCP6.0. Cutting emission factors in half but without diet change would also lower projected 2050 N2O to 252ppb. Adding 50

  20. Efficient conversion of crude glycerol from various industrial wastes into single cell oil by yeast Yarrowia lipolytica.

    PubMed

    Dobrowolski, Adam; Mituła, Paweł; Rymowicz, Waldemar; Mirończuk, Aleksandra M

    2016-05-01

    In this study, crude glycerol from various industries was used to produce lipids via wild type Yarrowia lipolytica A101. We tested samples without any prior purification from five different waste products; each contained various concentrations of glycerol (42-87%) as the sole carbon source. The best results for lipid production were obtained for medium containing glycerol from fat saponification. This reached 1.69gL(-1) (25% of total cell dry weight) with a biomass yield of 0.17gg(-1) in the flasks experiment. The batch cultivation in a bioreactor resulted in enhanced lipid production-it achieved 4.72gL(-1) with a biomass yield 0.21gg(-1). Moreover, the properly selected batch of crude glycerol provides a defined fatty acid composition. In summary, this paper shows that crude glycerol from soap production could be efficiently converted to single cell oil without any prior purification. PMID:26890799

  1. Efficient secretory production of CotA-laccase and its application in the decolorization and detoxification of industrial textile wastewater.

    PubMed

    Guan, Zheng-Bing; Shui, Yan; Song, Chen-Meng; Zhang, Ning; Cai, Yu-Jie; Liao, Xiang-Ru

    2015-06-01

    Fungal laccases are typically unstable at high pH and temperature conditions, which limit their application in the decolorization of textile wastewater. By contrast, the highly stable bacterial laccases can function within a wider pH range and at high temperatures, thus have significant potential in treatment for textile wastewater. In our previous work, a thermo-alkali-stable CotA-laccase gene was cloned from Bacillus pumilus W3 and overexpressed in Escherichia coli. In this study, the robust CotA-laccase achieved efficient secretory expression in Bacillus subtilis WB600 by screening a suitable signal peptide. A maximum CotA-laccase yield of 373.1 U/mL was obtained at optimum culture conditions in a 3-L fermentor. Furthermore, the decolorization and detoxification of textile industry effluent by the purified recombinant CotA-laccase in the presence and absence of redox mediators were investigated. Among the potential mediators that enhanced effluent decolorization, acetosyringone (ACS) was the most effective. The toxicity of the CotA-laccase-ACS-treated effluent was greatly reduced compared with that of the crude effluent. To the best of our knowledge, this study is the first to report on the heterologous expression of CotA-laccase in B. subtilis. The recombinant strain B. subtilis WB600-5 has a great potential in the industrial production of this bacterial enzyme, and the CotA-laccase-ACS system is a promising candidate for the biological treatment of industrial textile effluents. PMID:25847445

  2. The DOE s In-Plant Training (INPLT) Model to Promote Energy Efficiency in the Industrial Sector

    SciTech Connect

    Alkadi, Nasr E; Nimbalkar, Sachin U; De Fontaine, Mr. Andre; Schoeneborn, Fred C

    2013-01-01

    In-Plant Training (INPLT) is a new model for developing energy efficiency expertise within the US manufacturing companies participating in the U.S. Department of Energy s (DOE s) Better Buildings, Better Plants Program-a nationwide initiative to drive a 25% reduction in industrial energy intensity in 10 years. INPLTs are designed to fill a market niche by providing hands on training in a real world manufacturing plant environment. Through INPLTs, participants from multiple manufacturing plants, supply chains, utilities, and other external stakeholders learn how to conduct energy assessments, use energy analysis tools to analyze energy saving opportunities, develop energy management systems, and implement energy savings projects. Typical INPLT events are led by DOE-certified Energy Experts and range from 2-4 days. Topics discussed include: identification of cross-cutting or system specific opportunities; introduction to ISO 50001 Energy Management Systems; and energy project implementation and replication. This model is flexible, and can be tailored to suit the needs of specific industries. The INPLTs are a significant departure from the traditional single plant energy assessment model previously employed by DOE. INPLTs shift the focus from the concept of a single-plant s energy profile to a broader focus on training and capacity building among multiple industrial participants. The objective is to enable trainees to identify, quantify, implement and replicate future energy saving projects without continued external assistance. This paper discusses the INPLT model and highlights some of the initial outcomes from the successfully delivered INPLTs and the overall impact in terms of numbers of plants/participants trained, impacted energy footprints, and potential replication of identified opportunities.

  3. Energy Efficiency Improvement and Cost Saving Opportunities for the U.S. Iron and Steel Industry An ENERGY STAR(R) Guide for Energy and Plant Managers

    SciTech Connect

    Worrell, Ernst; Blinde, Paul; Neelis, Maarten; Blomen, Eliane; Masanet, Eric

    2010-10-21

    Energy is an important cost factor in the U.S iron and steel industry. Energy efficiency improvement is an important way to reduce these costs and to increase predictable earnings, especially in times of high energy price volatility. There are a variety of opportunities available at individual plants in the U.S. iron and steel industry to reduce energy consumption in a cost-effective manner. This Energy Guide discusses energy efficiency practices and energy-efficient technologies that can be implemented at the component, process, facility, and organizational levels. A discussion of the structure, production trends, energy consumption, and greenhouse gas emissions of the iron and steel industry is provided along with a description of the major process technologies used within the industry. Next, a wide variety of energy efficiency measures are described. Many measure descriptions include expected savings in energy and energy-related costs, based on case study data from real-world applications in the steel and related industries worldwide. Typical measure payback periods and references to further information in the technical literature are also provided, when available. The information in this Energy Guide is intended to help energy and plant managers in the U.S. iron and steel industry reduce energy consumption and greenhouse gas emissions in a cost-effective manner while maintaining the quality of products manufactured. Further research on the economics of all measures?and on their applicability to different production practices?is needed to assess their cost effectiveness at individual plants.

  4. RabGEF1/Rabex-5 Regulates TrkA-Mediated Neurite Outgrowth and NMDA-Induced Signaling Activation in NGF-Differentiated PC12 Cells

    PubMed Central

    Tam, See-Ying; Lilla, Jennifer N.; Chen, Ching-Cheng; Kalesnikoff, Janet; Tsai, Mindy

    2015-01-01

    Nerve growth factor (NGF) binds to its cognate receptor TrkA and induces neuronal differentiation by activating distinct downstream signal transduction events. RabGEF1 (also known as Rabex-5) is a guanine nucleotide exchange factor for Rab5, which regulates early endosome fusion and vesicular trafficking in endocytic pathways. Here, we used the antisense (AS) expression approach to induce an NGF-dependent sustained knockdown of RabGEF1 protein expression in stable PC12 transfectants. We show that RabGEF1 is a negative regulator of NGF-induced neurite outgrowth and modulates other cellular and signaling processes that are activated by the interaction of NGF with TrkA receptors, such as cell cycle progression, cessation of proliferation, and activation of NGF-mediated downstream signaling responses. Moreover, RabGEF1 can bind to Rac1, and the activation of Rac1 upon NGF treatment is significantly enhanced in AS transfectants, suggesting that RabGEF1 is a negative regulator of NGF-induced Rac1 activation in PC12 cells. Furthermore, we show that RabGEF1 can also interact with NMDA receptors by binding to the NR2B subunit and its associated binding partner SynGAP, and negatively regulates activation of nitric oxide synthase activity induced by NMDA receptor stimulation in NGF-differentiated PC12 cells. Our data suggest that RabGEF1 is a negative regulator of TrkA-dependent neuronal differentiation and of NMDA receptor-mediated signaling activation in NGF-differentiated PC12 cells. PMID:26588713

  5. Release of GTP Exchange Factor Mediated Down-Regulation of Abscisic Acid Signal Transduction through ABA-Induced Rapid Degradation of RopGEFs

    PubMed Central

    Waadt, Rainer; Schroeder, Julian I.

    2016-01-01

    The phytohormone abscisic acid (ABA) is critical to plant development and stress responses. Abiotic stress triggers an ABA signal transduction cascade, which is comprised of the core components PYL/RCAR ABA receptors, PP2C-type protein phosphatases, and protein kinases. Small GTPases of the ROP/RAC family act as negative regulators of ABA signal transduction. However, the mechanisms by which ABA controls the behavior of ROP/RACs have remained unclear. Here, we show that an Arabidopsis guanine nucleotide exchange factor protein RopGEF1 is rapidly sequestered to intracellular particles in response to ABA. GFP-RopGEF1 is sequestered via the endosome-prevacuolar compartment pathway and is degraded. RopGEF1 directly interacts with several clade A PP2C protein phosphatases, including ABI1. Interestingly, RopGEF1 undergoes constitutive degradation in pp2c quadruple abi1/abi2/hab1/pp2ca mutant plants, revealing that active PP2C protein phosphatases protect and stabilize RopGEF1 from ABA-mediated degradation. Interestingly, ABA-mediated degradation of RopGEF1 also plays an important role in ABA-mediated inhibition of lateral root growth. The presented findings point to a PP2C-RopGEF-ROP/RAC control loop model that is proposed to aid in shutting off ABA signal transduction, to counteract leaky ABA signal transduction caused by “monomeric” PYL/RCAR ABA receptors in the absence of stress, and facilitate signaling in response to ABA. PMID:27192441

  6. PDZ-RhoGEF and LARG Are Essential for Embryonic Development and Provide a Link between Thrombin and LPA Receptors and Rho Activation*

    PubMed Central

    Mikelis, Constantinos M.; Palmby, Todd R.; Simaan, May; Li, Wenling; Szabo, Roman; Lyons, Ruth; Martin, Daniel; Yagi, Hiroshi; Fukuhara, Shigetomo; Chikumi, Hiroki; Galisteo, Rebeca; Mukouyama, Yoh-suke; Bugge, Thomas H.; Gutkind, J. Silvio

    2013-01-01

    G protein-coupled receptors (GPCRs) linked to both members of the Gα12 family of heterotrimeric G proteins α subunits, Gα12 and Gα13, regulate the activation of Rho GTPases, thereby contributing to many key biological processes. Multiple Rho GEFs have been proposed to link Gα12/13 GPCRs to Rho activation, including PDZ-RhoGEF (PRG), leukemia-associated Rho GEF (LARG), p115-RhoGEF (p115), lymphoid blast crisis (Lbc), and Dbl. PRG, LARG, and p115 share the presence of a regulator of G protein signaling homology (RGS) domain. There is limited information on the biological roles of this RGS-containing family of RhoGEFs in vivo. p115-deficient mice are viable with some defects in the immune system and gastrointestinal motor dysfunctions, whereas in an initial study we showed that mice deficient for Larg are viable and resistant to salt-induced hypertension. Here, we generated knock-out mice for Prg and observed that these mice do not display any overt phenotype. However, deficiency in Prg and Larg leads to complex developmental defects and early embryonic lethality. Signaling from Gα11/q-linked GPCRs to Rho was not impaired in mouse embryonic fibroblasts defective in all three RGS-containing RhoGEFs. However, a combined lack of Prg, Larg, and p115 expression abolished signaling through Gα12/13 to Rho and thrombin-induced cell proliferation, directional migration, and nuclear signaling through JNK and p38. These findings provide evidence of an essential role for the RGS-containing RhoGEF family in signaling to Rho by Gα12/13-coupled GPCRs, which may likely play a critical role during embryonic development. PMID:23467409

  7. Supervillin Binds the Rac/Rho-GEF Trio and Increases Trio-mediated Rac1 Activation

    PubMed Central

    Son, Kyonghee; Smith, Tara C.; Luna, Elizabeth J.

    2015-01-01

    We investigated cross-talk between the membrane-associated, myosin II-regulatory protein supervillin and the actin-regulatory small GTPases Rac1, RhoA, and Cdc42. Supervillin knockdown reduced Rac1-GTP loading, but not the GTP loading of RhoA or Cdc42, in HeLa cells with normal levels of the Rac1-activating protein Trio. No reduction in Rac1-GTP loading was observed when supervillin levels were reduced in Trio-depleted cells. Conversely, overexpression of supervillin isoform 1 (SV1) or, especially, isoform 4 (SV4) increased Rac1 activation. Inhibition of the Trio-mediated Rac1 guanine nucleotide exchange (GEF) activity with ITX3 partially blocked the SV4-mediated increase in Rac1-GTP. Both SV4 and SV1 co-localized with Trio at or near the plasma membrane in ruffles and cell surface projections. Two sequences within supervillin bound directly to Trio spectrin repeats 4–7: SV1-171, which contains N-terminal residues found in both SV1 and SV4 and the SV4-specific differentially spliced coding exons 3, 4, and 5 within SV4 (SV4-E345; SV4 amino acids 276 – 669). In addition, SV4-E345 interacted with the homologous sequence in rat kalirin (repeats 4–7, amino acids 531 – 1101). Overexpressed SV1-174 and SV4-E345 affected Rac1-GTP loading, but only in cells with endogenous levels of Trio. Trio residues 771 – 1057, which contain both supervillin-interaction sites, exerted a dominant-negative effect on cell spreading. Supervillin and Trio knockdowns, separately or together, inhibited cell spreading, suggesting that supervillin regulates the Rac1 guanine nucleotide exchange activity of Trio, and potentially also kalirin, during cell spreading and lamellipodia extension. PMID:25655724

  8. Dictyostelium Dock180-related RacGEFs regulate the actin cytoskeleton during cell motility.

    PubMed

    Para, Alessia; Krischke, Miriam; Merlot, Sylvain; Shen, Zhouxin; Oberholzer, Michael; Lee, Susan; Briggs, Steven; Firtel, Richard A

    2009-01-01

    Cell motility of amoeboid cells is mediated by localized F-actin polymerization that drives the extension of membrane protrusions to promote forward movements. We show that deletion of either of two members of the Dictyostelium Dock180 family of RacGEFs, DockA and DockD, causes decreased speed of chemotaxing cells. The phenotype is enhanced in the double mutant and expression of DockA or DockD complements the reduced speed of randomly moving DockD null cells' phenotype, suggesting that DockA and DockD are likely to act redundantly and to have similar functions in regulating cell movement. In this regard, we find that overexpressing DockD causes increased cell speed by enhancing F-actin polymerization at the sites of pseudopod extension. DockD localizes to the cell cortex upon chemoattractant stimulation and at the leading edge of migrating cells and this localization is dependent on PI3K activity, suggesting that DockD might be part of the pathway that links PtdIns(3,4,5)P(3) production to F-actin polymerization. Using a proteomic approach, we found that DdELMO1 is associated with DockD and that Rac1A and RacC are possible in vivo DockD substrates. In conclusion, our work provides a further understanding of how cell motility is controlled and provides evidence that the molecular mechanism underlying Dock180-related protein function is evolutionarily conserved. PMID:19037099

  9. Using GEFS ensemble forecasts for decision making in reservoir management in California

    NASA Astrophysics Data System (ADS)

    Scheuerer, M.; Hamill, T.; Webb, R. S.

    2015-12-01

    Reservoirs such as Lake Mendocino in California's Russian River Basin provide flood control, water supply, recreation, and environmental stream flow regulation. Many of these reservoirs are operated by the U.S. Army Corps of Engineers (Corps) according to water control manuals that specify elevations for an upper volume of reservoir storage that must be kept available for capturing storm runoff and reducing flood risk, and a lower volume of storage that may be used for water supply. During extreme rainfall events, runoff is captured by these reservoirs and released as quickly as possible to create flood storage space for another potential storm. These flood control manuals are based on typical historical weather patterns - wet during the winter, dry otherwise - but are not informed directly by weather prediction. Alternative reservoir management approaches such as Forecast-Informed Reservoir Operations (FIRO), which seek to incorporate advances in weather prediction, are currently being explored as means to improve water supply availability while maintaining flood risk reduction and providing additional ecosystem benefits.We present results from a FIRO proof-of-concept study investigating the reliability of post-processed GEFS ensemble forecasts to predict the probability that day 6-to-10 precipitation accumulations in certain areas in California exceed a high threshold. Our results suggest that reliable forecast guidance can be provided, and the resulting probabilities could be used to inform decisions to release or hold water in the reservoirs. We illustrate the potential of these forecasts in a case study of extreme event probabilities for the Russian River Basin in California.

  10. Activated RhoA Binds to the Pleckstrin Homology (PH) Domain of PDZ-RhoGEF, a Potential Site for Autoregulation

    SciTech Connect

    Chen, Zhe; Medina, Frank; Liu, Mu-ya; Thomas, Celestine; Sprang, Stephen R.; Sternweis, Paul C.

    2010-07-19

    Guanine nucleotide exchange factors (GEFs) catalyze exchange of GDP for GTP by stabilizing the nucleotide-free state of the small GTPases through their Dbl homology/pleckstrin homology (DH {center_dot} PH) domains. Unconventionally, PDZ-RhoGEF (PRG), a member of the RGS-RhoGEFs, binds tightly to both nucleotide-free and activated RhoA (RhoA {center_dot} GTP). We have characterized the interaction between PRG and activated RhoA and determined the structure of the PRG-DH {center_dot} PH-RhoA {center_dot} GTP{gamma}S (guanosine 5{prime}-O-[{gamma}-thio]triphosphate) complex. The interface bears striking similarity to a GTPase-effector interface and involves the switch regions in RhoA and a hydrophobic patch in PRG-PH that is conserved among all Lbc RhoGEFs. The two surfaces that bind activated and nucleotide-free RhoA on PRG-DH {center_dot} PH do not overlap, and a ternary complex of PRG-DH {center_dot} PH bound to both forms of RhoA can be isolated by size-exclusion chromatography. This novel interaction between activated RhoA and PH could play a key role in regulation of RhoGEF activity in vivo.

  11. Dock10, a Cdc42 and Rac1 GEF, induces loss of elongation, filopodia, and ruffles in cervical cancer epithelial HeLa cells

    PubMed Central

    Ruiz-Lafuente, Natalia; Alcaraz-García, María-José; García-Serna, Azahara-María; Sebastián-Ruiz, Silvia; Moya-Quiles, María-Rosa; García-Alonso, Ana-María; Parrado, Antonio

    2015-01-01

    Dock10 is one of the three members of the Dock-D family of Dock proteins, a class of guanine nucleotide exchange factors (GEFs) for Rho GTPases. Its homologs Dock9 and Dock11 are Cdc42 GEFs. Dock10 is required for maintenance of rounded morphology and amoeboid-type movement. Full-length isoforms of Dock10 have been recently cloned. Here, we address GTPase specificity and GEF activity of Dock10. In order of decreasing intensity, Dock10 interacted with nucleotide-free Rac1, Cdc42, and Rac3, and more weakly with Rac2, RhoF, and RhoG. Inducible expression of Dock10 in HeLa epithelial cells promoted GEF activity on Cdc42 and Rac1, and a morphologic change in two-dimensional culture consisting in loss of cell elongation, increase of filopodia, and ruffles. Area in contact with the substrate of cells that spread with non-elongated morphology was larger in cells expressing Dock10. Inducible expression of constitutively active mutants of Cdc42 and Rac1 in HeLa cells also induced loss of elongation. However, Cdc42 induced filopodia and contraction, and Rac1 induced membrane ruffles and flattening. When co-expressed with Dock10, Cdc42 potentiated filopodia, and Rac1 potentiated ruffles. These results suggest that Dock10 functions as a dual GEF for Cdc42 and Rac1, affecting cell morphology, spreading and actin cytoskeleton protrusions of adherent HeLa cells. PMID:25862245

  12. Costs and benefits of industrial reporting and voluntary targets for energy efficiency. A report to the Congress of the United States. Volume II: Appendices

    SciTech Connect

    Not Available

    1994-02-01

    This part sets forth the regulations for the Industrial Energy conservation Program established under Part E of Title III of the Act. It includes criteria and procedures for the identification of reporting corporations, reporting requirements, criteria and procedures for exemption from filing reports directly with DOE, voluntary industrial energy efficiency improvement targets and voluntary recovered materials utilization targets. The purpose of the program is to promote increased energy conservation by American industry and, as it relates to the use of recovered materials, to conserve valuable energy and scarce natural resources.

  13. New process modeling [sic], design, and control strategies for energy efficiency, high product quality, and improved productivity in the process industries. Final project report

    SciTech Connect

    Ray, W. Harmon

    2002-06-05

    This project was concerned with the development of process design and control strategies for improving energy efficiency, product quality, and productivity in the process industries. In particular, (i) the resilient design and control of chemical reactors, and (ii) the operation of complex processing systems, was investigated. Specific topics studied included new process modeling procedures, nonlinear controller designs, and control strategies for multiunit integrated processes. Both fundamental and immediately applicable results were obtained. The new design and operation results from this project were incorporated into computer-aided design software and disseminated to industry. The principles and design procedures have found their way into industrial practice.

  14. The Guanine Nucleotide Exchange Factor (GEF) Asef2 Promotes Dendritic Spine Formation via Rac Activation and Spinophilin-dependent Targeting*

    PubMed Central

    Evans, J. Corey; Robinson, Cristina M.; Shi, Mingjian; Webb, Donna J.

    2015-01-01

    Dendritic spines are actin-rich protrusions that establish excitatory synaptic contacts with surrounding neurons. Reorganization of the actin cytoskeleton is critical for the development and plasticity of dendritic spines, which is the basis for learning and memory. Rho family GTPases are emerging as important modulators of spines and synapses, predominantly through their ability to regulate actin dynamics. Much less is known, however, about the function of guanine nucleotide exchange factors (GEFs), which activate these GTPases, in spine and synapse development. In this study we show that the Rho family GEF Asef2 is found at synaptic sites, where it promotes dendritic spine and synapse formation. Knockdown of endogenous Asef2 with shRNAs impairs spine and synapse formation, whereas exogenous expression of Asef2 causes an increase in spine and synapse density. This effect of Asef2 on spines and synapses is abrogated by expression of GEF activity-deficient Asef2 mutants or by knockdown of Rac, suggesting that Asef2-Rac signaling mediates spine development. Because Asef2 interacts with the F-actin-binding protein spinophilin, which localizes to spines, we investigated the role of spinophilin in Asef2-promoted spine formation. Spinophilin recruits Asef2 to spines, and knockdown of spinophilin hinders spine and synapse formation in Asef2-expressing neurons. Furthermore, inhibition of N-methyl-d-aspartate receptor (NMDA) activity blocks spinophilin-mediated localization of Asef2 to spines. These results collectively point to spinophilin-Asef2-Rac signaling as a novel mechanism for the development of dendritic spines and synapses. PMID:25750125

  15. Insights into the Molecular Activation Mechanism of the RhoA-specific Guanine Nucleotide Exchange Factor, PDZRhoGEF

    SciTech Connect

    Bielnicki, Jakub A.; Shkumatov, Alexander V.; Derewenda, Urszula; Somlyo, Avril V.; Svergun, Dmitri I.; Derewenda, Zygmunt S.

    2012-10-09

    PDZRhoGEF (PRG) belongs to a small family of RhoA-specific nucleotide exchange factors that mediates signaling through select G-protein-coupled receptors via G{alpha}{sub 12/13} and activates RhoA by catalyzing the exchange of GDP to GTP. PRG is a multidomain protein composed of PDZ, regulators of G-protein signaling-like (RGSL), Dbl-homology (DH), and pleckstrin-homology (PH) domains. It is autoinhibited in cytosol and is believed to undergo a conformational rearrangement and translocation to the membrane for full activation, although the molecular details of the regulation mechanism are not clear. It has been shown recently that the main autoregulatory elements of PDZRhoGEF, the autoinhibitory 'activation box' and the 'GEF switch,' which is required for full activation, are located directly upstream of the catalytic DH domain and its RhoA binding surface, emphasizing the functional role of the RGSL-DH linker. Here, using a combination of biophysical and biochemical methods, we show that the mechanism of PRG regulation is yet more complex and may involve an additional autoinhibitory element in the form of a molten globule region within the linker between RGSL and DH domains. We propose a novel, two-tier model of autoinhibition where the activation box and the molten globule region act synergistically to impair the ability of RhoA to bind to the catalytic DH-PH tandem. The molten globule region and the activation box become less ordered in the PRG-RhoA complex and dissociate from the RhoA-binding site, which may constitute a critical step leading to PRG activation.

  16. Energy Efficiency Improvement and Cost Saving Opportunities for the Petrochemical Industry - An ENERGY STAR(R) Guide for Energy and Plant Managers

    SciTech Connect

    Neelis, Maarten; Worrell, Ernst; Masanet, Eric

    2008-09-01

    Energy is the most important cost factor in the U.S petrochemical industry, defined in this guide as the chemical industry sectors producing large volume basic and intermediate organic chemicals as well as large volume plastics. The sector spent about $10 billion on fuels and electricity in 2004. Energy efficiency improvement is an important way to reduce these costs and to increase predictable earnings, especially in times of high energy price volatility. There are a variety of opportunities available at individual plants in the U.S. petrochemical industry to reduce energy consumption in a cost-effective manner. This Energy Guide discusses energy efficiency practices and energy efficient technologies that can be implemented at the component, process, facility, and organizational levels. A discussion of the trends, structure, and energy consumption characteristics of the petrochemical industry is provided along with a description of the major process technologies used within the industry. Next, a wide variety of energy efficiency measures are described. Many measure descriptions include expected savings in energy and energy-related costs, based on case study data from real-world applications in the petrochemical and related industries worldwide. Typical measure payback periods and references to further information in the technical literature are also provided, when available. The information in this Energy Guide is intended to help energy and plant managers in the U.S. petrochemical industry reduce energy consumption in a cost-effective manner while maintaining the quality of products manufactured. Further research on the economics of all measures--and on their applicability to different production practices--is needed to assess their cost effectiveness at individual plants.

  17. Glass Industry of the Future: Resources and Tools for Energy Efficiency and Cost Reduction Now (CD-ROM)

    SciTech Connect

    Not Available

    2003-08-01

    U. S. DOEs Industrial Technologies Programs Industries of the Future CD highlighting case studies, tips, software, and training opportunities to help glass companies control energy consumption and reduce costs.

  18. Energy Efficiency Improvement and Cost Saving Opportunities for the Fruit and Vegetable Processing Industry. An ENERGY STAR Guide for Energy and Plant Managers

    SciTech Connect

    Masanet, Eric; Masanet, Eric; Worrell, Ernst; Graus, Wina; Galitsky, Christina

    2008-01-01

    The U.S. fruit and vegetable processing industry--defined in this Energy Guide as facilities engaged in the canning, freezing, and drying or dehydrating of fruits and vegetables--consumes over $800 million worth of purchased fuels and electricity per year. Energy efficiency improvement isan important way to reduce these costs and to increase predictable earnings, especially in times of high energy price volatility. There are a variety of opportunities available at individual plants in the U.S. fruit and vegetable processing industry to reduce energy consumption in a cost-effective manner. This Energy Guide discusses energy efficiency practices and energy-efficient technologies that can be implemented at the component, process, facility, and organizational levels. A discussion of the trends, structure, and energy consumption characteristics of the U.S. fruit and vegetable processing industry is provided along with a description of the major process technologies used within the industry. Next, a wide variety of energy efficiency measures applicable to fruit and vegetable processing plants are described. Many measure descriptions include expected savings in energy and energy-related costs, based on case study data from real-world applications in fruit and vegetable processing facilities and related industries worldwide. Typical measure payback periods and references to further information in the technical literature are also provided, when available. Given the importance of water in fruit and vegetable processing, a summary of basic, proven measures for improving plant-level water efficiency are also provided. The information in this Energy Guide is intended to help energy and plant managers in the U.S. fruit and vegetable processing industry reduce energy and water consumption in a cost-effective manner while maintaining the quality of products manufactured. Further research on the economics of all measures--as well as on their applicability to different production

  19. GEFS+ is not related to the most common mutations of SCN1B, SCN1A and GABRG2 in two Tunisian families.

    PubMed

    Mrabet, H; Belhedi, N; Bouchlaka, S; El Gaaied, A; Mrabet, A

    2007-12-01

    The objective was to investigate whether the described mutations of the SCN1A, SCN1B and GABRG2 genes are associated to generalised epilepsy with febrile seizure plus (GEFS+) in two Tunisian families. We performed a genetic study of two multigenerational Tunisian families with GEFS+ spectrum. The molecular analysis included a PCR amplification of SCN1B, SCN1A and GAGRG2 exons, then a screening of the known SCN1B, SCN1A and GABRG2 gene mutations by direct sequencing. The data excluded the involvement of all known published mutations. However, an insertion of a T nucleotide at a heterozygous state within the intron 12 of the SCN1A gene has been identified in two probands and their parents. Our results corroborate the genetic heterogeneity of GEFS+ predominantly in epilepsy patients of different countries and ethnic groups. PMID:18175077

  20. The rice bright green leaf (bgl) locus encodes OsRopGEF10, which activates the development of small cuticular papillae on leaf surfaces.

    PubMed

    Yoo, Jeong-Hoon; Park, Jong-Ho; Cho, Sung-Hwan; Yoo, Soo-Cheul; Li, Jinjie; Zhang, Haitao; Kim, Kwang-Soo; Koh, Hee-Jong; Paek, Nam-Chon

    2011-12-01

    Development of specialized epidermal cells and structures plays a key role in plant tolerance to biotic and abiotic stresses. In the paddy field, the bright green leaf (bgl) mutants of rice (Oryza sativa) exhibit a luminous green color that is clearly distinguishable from the normal green of wild-type plants. Transmission and scanning electron microscopy revealed that small cuticular papillae (or small papillae; SP), nipple-like structures, are absent on the adaxial and abaxial leaf surfaces of bgl mutants, leading to more direct reflection and less diffusion of green light. Map-based cloning revealed that the bgl locus encodes OsRopGEF10, one of eleven OsRopGEFs in rice. RopGEFs (guanine nucleotide exchange factors for Rop) activate Rop/Rac GTPases, acting as molecular switches in eukaryotic signal transduction by replacing the bound GDP (inactive form) with GTP (active form) in response to external or internal cues. In agreement with the timing of SP initiation on the leaf epidermis, OsRopGEF10 is most strongly expressed in newly developing leaves before emergence from the leaf sheath. In yeast two-hybrid assays, OsRopGEF10 interacts with OsRac1, one of seven OsRac proteins; consistent with this, both proteins are localized in the plasma membrane. These results suggest that OsRopGEF10 activates OsRac1 to turn on the molecular signaling pathway for SP development. Together, our findings provide new insights into the molecular genetic mechanism of SP formation during early leaf morphogenesis. PMID:22038138

  1. Energy efficiency improvement and cost saving opportunities for the Corn Wet Milling Industry: An ENERGY STAR Guide for Energy and Plant Managers

    SciTech Connect

    Galitsky, Christina; Worrell, Ernst; Ruth, Michael

    2003-07-01

    Corn wet milling is the most energy intensive industry within the food and kindred products group (SIC 20), using 15 percent of the energy in the entire food industry. After corn, energy is the second largest operating cost for corn wet millers in the United States. A typical corn wet milling plant in the United States spends approximately $20 to $30 million per year on energy, making energy efficiency improvement an important way to reduce costs and increase predictable earnings, especially in times of high energy-price volatility. This report shows energy efficiency opportunities available for wet corn millers. It begins with descriptions of the trends, structure and production of the corn wet milling industry and the energy used in the milling and refining process. Specific primary energy savings for each energy efficiency measure based on case studies of plants and references to technical literature are provided. If available, typical payback periods are also listed. The report draws upon the experiences of corn, wheat and other starch processing plants worldwide for energy efficiency measures. The findings suggest that given available resources and technology, there are opportunities to reduce energy consumption cost-effectively in the corn wet milling industry while maintaining the quality of the products manufactured. Further research on the economics of the measures, as well as the applicability of these to different wet milling practices, is needed to assess the feasibility of implementation of selected technologies at individual plants.

  2. Save Energy Now (SEN) Assessment Helps Expand Energy Management Program at Shaw Industries: Flooring Company Saves $872,000 Annually by Improving Steam System Efficiency

    SciTech Connect

    Not Available

    2008-07-01

    This case study describes how the Shaw Industries plant #20 in Dalton, Georgia, achieved annual savings of $872,000 and 93,000 MMBtu after receiving a DOE Save Energy Now energy assessment and implementing recommendations to improve the efficiency of its steam system.

  3. Activation of p115-RhoGEF Requires Direct Association of G[alpha subscript 13] and the Dbl Homology Domain

    SciTech Connect

    Chen, Zhe; Guo, Liang; Hadas, Jana; Gutowski, Stephen; Sprang, Stephen R.; Sternweis, Paul C.

    2012-09-05

    RGS-containing RhoGEFs (RGS-RhoGEFs) represent a direct link between the G{sub 12} class of heterotrimeric G proteins and the monomeric GTPases. In addition to the canonical Dbl homology (DH) and pleckstrin homology domains that carry out the guanine nucleotide exchange factor (GEF) activity toward RhoA, these RhoGEFs also possess RGS homology (RH) domains that interact with activated {alpha} subunits of G{sub 12} and G{sub 13}. Although the GEF activity of p115-RhoGEF (p115), an RGS-RhoGEF, can be stimulated by G{alpha}{sub 13}, the exact mechanism of the stimulation has remained unclear. Using combined studies with small angle x-ray scattering, biochemistry, and mutagenesis, we identify an additional binding site for activated G{alpha}{sub 13} in the DH domain of p115. Small angle x-ray scattering reveals that the helical domain of G{alpha}{sub 13} docks onto the DH domain, opposite to the surface of DH that binds RhoA. Mutation of a single tryptophan residue in the {alpha}3b helix of DH reduces binding to activated G{alpha}{sub 13} and ablates the stimulation of p115 by G{alpha}{sub 13}. Complementary mutations at the predicted DH-binding site in the {alpha}B-{alpha}C loop of the helical domain of G{alpha}{sub 13} also affect stimulation of p115 by G{alpha}{sub 13}. Although the GAP activity of p115 is not required for stimulation by G{alpha}{sub 13}, two hydrophobic motifs in RH outside of the consensus RGS box are critical for this process. Therefore, the binding of G{alpha}{sub 13} to the RH domain facilitates direct association of G{alpha}{sub 13} to the DH domain to regulate its exchange activity. This study provides new insight into the mechanism of regulation of the RGS-RhoGEF and broadens our understanding of G protein signaling.

  4. Gamma and x-ray irradiation effects on different Ge and Ge/F doped optical fibers

    SciTech Connect

    Alessi, A. Girard, S.; Di Francesca, D.; Boukenter, A.; Ouerdane, Y.; Reghioua, I.; Fanetti, M.; Martin-Samos, L.; Agnello, S.; Cannas, M.; Marcandella, C.; Richard, N.

    2015-08-28

    We performed electron paramagnetic resonance (EPR) measurements on γ and X ray irradiated Ge doped and Ge/F co-doped optical fibers. We considered three different drawing conditions (speed and tension), and for each type of drawing, we studied Ge and Ge/F doped samples having Ge doping level above 4% by weight. The EPR data recorded for the γ ray irradiated fibers confirm that all the samples exhibit a very close radiation response regardless of the drawing conditions corresponding to values used for the production of specialty fibers. Furthermore, as for the X irradiated materials, in the γ ray irradiated F co-doped fibers, we observed that the Ge(1) and the Ge(2) defects generation is unchanged, whereas it was enhanced for the E'Ge. In the various fibers, the comparison of the γ and X-ray induced concentrations of these kinds of Ge related defects indicates that the two irradiations induce similar effects regardless of the different employed dose rates and sources. Confocal microscopy luminescence results show that the starting content of the Germanium Lone Pair Center (GLPC) is neither strongly affected by the Ge content nor by the drawing conditions, and we consider the similarity of the GLPC content as key factor in determining many of the above reported similarities.

  5. The first genome-wide scan in a tunisian family with generalized epilepsy with febrile seizure plus (GEFS+).

    PubMed

    Fendri-Kriaa, Nourhene; Louhichi, Nacim; Mkaouar-Rebai, Emna; Chabchoub, Ghazi; Kammoun, Fatma; Salem, Ikhlass Hadj; Rebai, Ahmed; Triki, Chahnez; Fakhfakh, Faiza

    2010-11-01

    Generalized epilepsy with febrile seizure plus (GEFS+) is an autosomal dominant disorder. In the literature, 5 responsible genes were identified and 2 novel susceptibility loci for GEFS+ at 2p24 and 8p23-p21 were reported, indicating the genetic heterogeneity of this disorder. The aim of this report is to identify the responsible loci in a large affected Tunisian family by performing a 10cM density genome-wide scan. The highest multipoint logarithm of odds (LOD) score (1.04) was found for D5S407 in the absence of recombination. Two other interesting regions were found around marker D19S210 (LOD=0.799) and D7S484 (LOD=0.61) markers. To fine map these loci, additional markers in 2 regions on 5q13.3 and 7p14.2 were analyzed and positive LOD scores for both loci were obtained. Sequencing of the Sodium channel subunit beta-1 gene (SCN1B) (19q13.1) showed the absence of any causal mutation. Our findings emphasized the genetic heterogeneity of febrile seizures. PMID:20382841

  6. Macroscopic stiffening of embryonic tissues via microtubules, RhoGEF and the assembly of contractile bundles of actomyosin

    PubMed Central

    Zhou, Jian; Kim, Hye Young; Wang, James H.-C.; Davidson, Lance A.

    2010-01-01

    During morphogenesis, forces generated by cells are coordinated and channeled by the viscoelastic properties of the embryo. Microtubules and F-actin are considered to be two of the most important structural elements within living cells accounting for both force production and mechanical stiffness. In this paper, we investigate the contribution of microtubules to the stiffness of converging and extending dorsal tissues in Xenopus laevis embryos using cell biological, biophysical and embryological techniques. Surprisingly, we discovered that depolymerizing microtubules stiffens embryonic tissues by three- to fourfold. We attribute tissue stiffening to Xlfc, a previously identified RhoGEF, which binds microtubules and regulates the actomyosin cytoskeleton. Combining drug treatments and Xlfc activation and knockdown lead us to the conclusion that mechanical properties of tissues such as viscoelasticity can be regulated through RhoGTPase pathways and rule out a direct contribution of microtubules to tissue stiffness in the frog embryo. We can rescue nocodazole-induced stiffening with drugs that reduce actomyosin contractility and can partially rescue morphogenetic defects that affect stiffened embryos. We support these conclusions with a multi-scale analysis of cytoskeletal dynamics, tissue-scale traction and measurements of tissue stiffness to separate the role of microtubules from RhoGEF activation. These findings suggest a re-evaluation of the effects of nocodazole and increased focus on the role of Rho family GTPases as regulators of the mechanical properties of cells and their mechanical interactions with surrounding tissues. PMID:20630946

  7. Degenerate specificity of PDZ domains from RhoA-specific nucleotide exchange factors PDZRhoGEF and LARG.

    PubMed

    Smietana, Katarzyna; Kasztura, Monika; Paduch, Marcin; Derewenda, Urszula; Derewenda, Zygmunt S; Otlewski, Jacek

    2008-01-01

    PDZ domains are ubiquitous protein-protein interaction modules which bind short, usually carboxyterminal fragments of receptors, other integral or membrane-associated proteins, and occasionally cytosolic proteins. Their role in organizing multiprotein complexes at the cellular membrane is crucial for many signaling pathways, but the rules defining their binding specificity are still poorly understood and do not readily explain the observed diversity of their known binding partners. Two homologous RhoA-specific, multidomain nucleotide exchange factors PDZRhoGEF and LARG contain PDZ domains which show a particularly broad recognition profile, as suggested by the identification of five diverse biological targets. To investigate the molecular roots of this phenomenon, we constructed a phage display library of random carboxyterminal hexapeptides. Peptide variants corresponding to the sequences identified in library selection were synthesized and their affinities for both PDZ domains were measured and compared with those of peptides derived from sequences of natural partners. Based on the analysis of the binding sequences identified for PDZRhoGEF, we propose a sequence for an 'optimal' binding partner. Our results support the hypothesis that PDZ-peptide interactions may be best understood when one considers the sum of entropic and dynamic effects for each peptide as a whole entity, rather than preferences for specific residues at a given position. PMID:18542831

  8. GTP-binding of ARL-3 is activated by ARL-13 as a GEF and stabilized by UNC-119

    PubMed Central

    Zhang, Qing; Li, Yan; Zhang, Yuxia; Torres, Vicente E.; Harris, Peter C.; Ling, Kun; Hu, Jinghua

    2016-01-01

    Primary cilia are sensory organelles indispensable for organogenesis and tissue pattern formation. Ciliopathy small GTPase ARLs are proposed as prominent ciliary switches, which when disrupted result in dysfunctional cilia, yet how ARLs are activated remain elusive. Here, we discover a novel small GTPase functional module, which contains ARL-3, ARL-13, and UNC-119, localizes near the poorly understood inversin (InV)-like compartment in C. elegans. ARL-13 acts synergistically with UNC-119, but antagonistically with ARL-3, in regulating ciliogenesis. We demonstrate that ARL-3 is a unique small GTPase with unusual high intrinsic GDP release but low intrinsic GTP binding rate. Importantly, ARL-13 acts as a nucleotide exchange factor (GEF) of ARL-3, while UNC-119 can stabilize the GTP binding of ARL-3. We further show that excess inactivated ARL-3 compromises ciliogenesis. The findings reveal a novel mechanism that one ciliopathy GTPase ARL-13, as a GEF, coordinates with UNC-119, which may act as a GTP-binding stabilizing factor, to properly activate another GTPase ARL-3 in cilia, a regulatory process indispensable for ciliogenesis. PMID:27102355

  9. Inhibition of RAC1-GEF DOCK3 by miR-512-3p contributes to suppression of metastasis in non-small cell lung cancer.

    PubMed

    Zhu, Xingli; Gao, Guanghui; Chu, Kaili; Yang, Xiufang; Ren, Shengxiang; Li, Yao; Wu, Hai; Huang, Yan; Zhou, Caicun

    2015-04-01

    MicroRNAs are a class of small non-coding RNAs regulating gene expression. In this study, we demonstrated that retinoic acid (RA) treatment increases the expression of miR-512-3p. Overexpression of miR-512-3p inhibited cell adhesion, migration, and invasion in non-small cell lung cancer (NSCLC) cell lines A549 and H1299. miR-512-3p inhibitor partially reversed these effects in H1299 cells stably expressing miR-512. We identified DOCK3, a RAC1-GEF (guanine nucleotide exchange factor), as a target gene of miR-512-3p. Overexpression of miR-512-3p led to the decrease of DOCK3 protein but not its mRNA. Knockdown of DOCK3 resulted in similar effects on adhesion, migration, and invasion as observed of miR-512-3p overexpression. Active RAC1 pull-down assay indicated that overexpression of miR-512-3p could decrease the activity of RAC1 with a higher efficiency than that of DOCK3 knockdown. Furthermore, expression of miR-512-3p was suppressed in most NSCLC patient tumor samples compared to its paired normal controls, suggesting that miR-512-3p might play a crucial role in lung cancer development. In conclusion, our results supported that miR-512-3p could inhibit tumor cell adhesion, migration, and invasion by regulating the RAC1 activity via DOCK3 in NSCLC A549 and H1299 cell lines. PMID:25687035

  10. Transforming on-grid renewable energy markets. A review of UNDP-GEF support for feed-in tariffs and related price and market-access instruments

    SciTech Connect

    Glemarec, Yannick; Rickerson, Wilson; Waissbein, Oliver

    2012-11-15

    As a Global Environment Facility (GEF) founding implementing agency, UNDP has worked on over 230 GEF-supported clean energy projects in close to 100 developing countries since 1992. About 100 of these projects in 80 countries have focused on renewable energy, supported by approximately US $ 293 million in GEF funds and leveraging US $1.48 billion in associated co-financing from national governments, international organizations, the private sector and non-governmental organizations. As part of UNDP efforts to codify and share lessons learnt from these initiatives, this report addresses how scarce public resources can be used to catalyze larger private financial flows for renewable energy. It provides an overview of UNDP-GEF’s extensive work supporting development of national renewable energy policies such as feed-in tariffs. In these activities UNDP-GEF assists developing countries to assess key risks and barriers to technology diffusion and then to identify a mix of policy and financial de-risking measures to remove these barriers and drive investment. This approach is illustrated through three case studies in Uruguay, Mauritius and Kazakhstan. This report is complemented by a companion publication presenting an innovative UNDP financial modeling tool to assist policymakers in appraising different public instruments to promote clean energy.

  11. Inducible RasGEF1B circular RNA is a positive regulator of ICAM-1 in the TLR4/LPS pathway.

    PubMed

    Ng, Wei Lun; Marinov, Georgi K; Liau, Ee Shan; Lam, Yi Lyn; Lim, Yat-Yuen; Ea, Chee-Kwee

    2016-09-01

    Circular RNAs (circRNAs) constitute a large class of RNA species formed by the back-splicing of co-linear exons, often within protein-coding transcripts. Despite much progress in the field, it remains elusive whether the majority of circRNAs are merely aberrant splicing by-products with unknown functions, or their production is spatially and temporally regulated to carry out specific biological functions. To date, the majority of circRNAs have been cataloged in resting cells. Here, we identify an LPS-inducible circRNA: mcircRasGEF1B, which is predominantly localized in cytoplasm, shows cell-type specific expression, and has a human homolog with similar properties, hcircRasGEF1B. We show that knockdown of the expression of mcircRasGEF1B reduces LPS-induced ICAM-1 expression. Additionally, we demonstrate that mcircRasGEF1B regulates the stability of mature ICAM-1 mRNAs. These findings expand the inventory of functionally characterized circRNAs with a novel RNA species that may play a critical role in fine-tuning immune responses and protecting cells against microbial infection. PMID:27362560

  12. High-Efficiency, Cost-effective Thermoelectric Materials/Devices for Industrial Process Refrigeration and Waste Heat Recovery, STTR Phase II Final Report

    SciTech Connect

    Lin, Timothy

    2011-01-07

    This is the final report of DoE STTR Phase II project, “High-efficiency, Cost-effective Thermoelectric Materials/Devices for Industrial Process Refrigeration and Waste Heat Recovery”. The objective of this STTR project is to develop a cost-effective processing approach to produce bulk high-performance thermoelectric (TE) nanocomposites, which will enable the development of high-power, high-power-density TE modulus for waste heat recovery and industrial refrigeration. The use of this nanocomposite into TE modules are expected to bring about significant technical benefits in TE systems (e.g. enhanced energy efficiency, smaller sizes and light weight). The successful development and applications of such nanocomposite and the resultant TE modules can lead to reducing energy consumption and environmental impacts, and creating new economic development opportunities.

  13. Does a SCN1A gene mutation confer earlier age of onset of febrile seizures in GEFS+?

    PubMed

    Sijben, Angelique E J; Sithinamsuwan, Pasiri; Radhakrishnan, Ashalata; Badawy, Radwa A B; Dibbens, Leanne; Mazarib, Aziz; Lev, Dorit; Lerman-Sagie, Tally; Straussberg, Rachel; Berkovic, Samuel F; Scheffer, Ingrid E

    2009-04-01

    SCN1A is the most clinically relevant epilepsy gene and is associated with generalized epilepsy and febrile seizure plus (GEFS+) and Dravet syndrome. We postulated that earlier onset of febrile seizures in the febrile seizure (FS) and febrile seizure plus (FS+) phenotypes may occur in the presence of a SCN1A mutation. This was because of the age-related onset of Dravet syndrome, which typically begins in the first year of life. We found that patients with FS and FS+ with SCN1A mutations had earlier median onset of febrile seizures compared to the population median. Patients with GABRG2 mutations had a similar early onset in contrast to patients with SCN1B mutations where onset was later. This study is the first to demonstrate that a specific genetic abnormality directly influences the FS and FS+ phenotype in terms of age of onset. PMID:19292758

  14. Industrial innovations for tomorrow: Advances in industrial energy-efficiency technologies. Commercial power plant tests blend of refuse-derived fuel and coal to generate electricity

    SciTech Connect

    Not Available

    1993-11-01

    MSW can be converted to energy in two ways. One involves the direct burning of MSW to produce steam and electricity. The second converts MSW into refuse-derived fuel (RDF) by reducing the size of the MSW and separating metals, glass, and other inorganic materials. RDF can be densified or mixed with binders to form fuel pellets. As part of a program sponsored by DOE`s Office of Industrial Technologies, the National Renewable Energy Laboratory participated in a cooperative research and development agreement to examine combustion of binder-enhanced, densified refuse-derived fuel (b-d RDF) pellets with coal. Pelletized b-d RDF has been burned in coal combustors, but only in quantities of less than 3% in large utility systems. The DOE project involved the use of b-d RDF in quantities up to 20%. A major goal was to quantify the pollutants released during combustion and measure combustion performance.

  15. Germ Cell Segregation from the Drosophila Soma Is Controlled by an Inhibitory Threshold Set by the Arf-GEF Steppke

    PubMed Central

    Lee, Donghoon M.; Wilk, Ronit; Hu, Jack; Krause, Henry M.; Harris, Tony J. C.

    2015-01-01

    Germline cells segregate from the soma to maintain their totipotency, but the cellular mechanisms of this segregation are unclear. The Drosophila melanogaster embryo forms a posterior group of primordial germline cells (PGCs) by their division from the syncytial soma. Extended plasma membrane furrows enclose the PGCs in response to the germ plasm protein Germ cell-less (Gcl) and Rho1–actomyosin activity. Recently, we found that loss of the Arf-GEF Steppke (Step) leads to similar Rho1-dependent plasma membrane extensions but from pseudocleavage furrows of the soma. Here, we report that the loss of step also leads to premature formation of a large cell group at the anterior pole of the embryo . These anterior cells lacked germ plasm, but budded and formed at the same time as posterior PGCs, and then divided asynchronously as PGCs also do. With genetic analyses we found that Step normally activates Arf small G proteins and antagonizes Rho1–actomyosin pathways to inhibit anterior cell formation. A uniform distribution of step mRNA around the one-cell embryo cortex suggested that Step restricts cell formation through a global control mechanism. Thus, we examined the effect of Step on PGC formation at the posterior pole. Reducing Gcl or Rho1 levels decreased PGC numbers, but additional step RNAi restored their numbers. Reciprocally, GFP–Step overexpression induced dosage- and Arf-GEF-dependent loss of PGCs, an effect worsened by reducing Gcl or actomyosin pathway activity. We propose that a global distribution of Step normally sets an inhibitory threshold for Rho1 activity to restrict early cell formation to the posterior. PMID:25971667

  16. Whole-genome sequencing of the efficient industrial fuel-ethanol fermentative Saccharomyces cerevisiae strain CAT-1.

    PubMed

    Babrzadeh, Farbod; Jalili, Roxana; Wang, Chunlin; Shokralla, Shadi; Pierce, Sarah; Robinson-Mosher, Avi; Nyren, Pål; Shafer, Robert W; Basso, Luiz C; de Amorim, Henrique V; de Oliveira, Antonio J; Davis, Ronald W; Ronaghi, Mostafa; Gharizadeh, Baback; Stambuk, Boris U

    2012-06-01

    The Saccharomyces cerevisiae strains widely used for industrial fuel-ethanol production have been developed by selection, but their underlying beneficial genetic polymorphisms remain unknown. Here, we report the draft whole-genome sequence of the S. cerevisiae strain CAT-1, which is a dominant fuel-ethanol fermentative strain from the sugarcane industry in Brazil. Our results indicate that strain CAT-1 is a highly heterozygous diploid yeast strain, and the ~12-Mb genome of CAT-1, when compared with the reference S228c genome, contains ~36,000 homozygous and ~30,000 heterozygous single nucleotide polymorphisms, exhibiting an uneven distribution among chromosomes due to large genomic regions of loss of heterozygosity (LOH). In total, 58 % of the 6,652 predicted protein-coding genes of the CAT-1 genome constitute different alleles when compared with the genes present in the reference S288c genome. The CAT-1 genome contains a reduced number of transposable elements, as well as several gene deletions and duplications, especially at telomeric regions, some correlated with several of the physiological characteristics of this industrial fuel-ethanol strain. Phylogenetic analyses revealed that some genes were likely associated with traits important for bioethanol production. Identifying and characterizing the allelic variations controlling traits relevant to industrial fermentation should provide the basis for a forward genetics approach for developing better fermenting yeast strains. PMID:22562254

  17. The Applied and Industrial Mathematics Program with Example Project: Efficient Routing for Meals on Wheels in the Shenandoah Valley

    ERIC Educational Resources Information Center

    Axvig, Nathan; David, John; Falcetti, Alex

    2015-01-01

    The Applied and Industrial Mathematics program partners applied mathematics students with businesses, governments, laboratories, and agencies to solve real problems. We will discuss the logistics of the program and give advice on starting and running similar programs. We also give a detailed example of a specific project, namely our work with the…

  18. Industrial-scale radio frequency treatments for insect control in walnuts I. Heating uniformity and energy efficiency

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Conducting industrial-scale confirmatory treatments is the final step in developing commercially and environmentally sound insect control technologies for in-shell walnuts using radio frequency (RF) energy as an alternative to chemical fumigation. Improving heating uniformity of in-shell walnuts in ...

  19. Impact of Fibrous Coproducts From the Ethanol Industry on Pig Growth, Feed Efficiency, Health, and Carcass Characteristics

    Technology Transfer Automated Retrieval System (TEKTRAN)

    With the increasing production of biofuels from typical dietary energy sources, such as corn and vegetable oils, the interest in using alternative feedstuffs has grown. Further adding to this interest, is the increased production and availability of coproducts from the biofuels industry. Since the s...

  20. 76 FR 9329 - Efficiency Initiative Effort To Reduce Non-Value-Added Costs Imposed on Industry by Department of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-17

    ...) Dr. Ashton Carter, dated September 14, 2010, Memorandum to Acquisition Professionals, DoD is... first stage of Undersecretary Carter's Better Buying Power Initiative. Many of these suggestions were... Undersecretary Carter's Efficiency Initiative. Trade secrets and commercial or financial information...

  1. [Remediation efficiency of lead-contaminated soil at an industrial site by ultrasonic-assisted chemical extraction].

    PubMed

    Wang, Xin-jie; Huang, Jin-lou; Liu, Zhi-qiang; Yue, Xi

    2013-09-01

    This research chose five lead-contaminated sites of a lead-acid battery factory to analyze the speciation distribution and concentration of lead. Under the same conditions (0.1 mol x L(-1) EDTA,30 min, 25 degrees C), the removal effect of heavy metal was compared between ultrasonic-assisted chemical extraction (UCE) and conventional chemical extraction ( CCE), and the variation of lead speciation was further explored. The results showed that the lead removal efficiency of UCE was significantly better than CCE. The lead removal efficiency of WS, A, B, C and BZ was 10.06%, 48.29%, 48.69%, 53.28% and 36.26% under CCE. While the removal efficiency of the UCE was 22.42%, 69.31%, 71.00%, 74.49% and 71.58%, with the average efficiency higher by 22%. By comparing the speciation distribution of the two washing methods, it was found that the acid extractable content maintained or decreased after UCE, whereas it showed an increasing trend after CCE. The reduction effect of the reducible was as high as 98% by UCE. UCE also showed a more efficient reduction effect of the organic matter-sulfite bounded form and the residual form. Hence, it is feasible to improve the washing efficiency of heavy metal contained in soil by conducting the cleaning process with the help of ultrasonic wave, which is a simple and fast mean to remove lead from contaminated sites. PMID:24289027

  2. Finding of no significant impact for the joint DOE/EPA program on national industrial competitiveness through energy efficiency and economics (NICE{sup 3})

    SciTech Connect

    1997-03-01

    The Department of Energy (DOE) has prepared a Programmatic Environmental Assessment (PEA), to assess the environment impacts associated with a joint DOE/EPA cost-sharing grant program named National Industrial Competitiveness through Energy Efficiency, Environment and Economics (NICE{sup 3}). The purpose of the NICE{sup 3} Program is to encourage waste minimization technology in industry by funding projects that develop activities and process improvements to conserve energy and reduce pollution. The proposed action would provide Federal financial assistance in the form of grants to industry in order to promote pollution prevention, energy efficiency, and cost competitiveness. Based on the analysis presented in the PEA, DOE has determined that the proposed action (providing NICE{sup 3} grants for projects which are consistent with the goals of the PPA and EPACT) does not constitute a major Federal action significantly affecting the quality of the human environment within the meaning of NEPA. Therefore, the preparation of an Environmental Impact Statement is not needed and the Department is issuing this Finding of No Significant Impact.

  3. Energy-efficient technology in the iron and steel industry: Simulation of new technology adoption with items

    SciTech Connect

    Roop, J.M.

    1997-07-01

    The Industrial Technology and Energy Modeling System (ITEMS)(referred to as ISTUM in Jaccard and Roop, 1990) is an end-use industrial modeling system that is technology based. Because it includes technologies in the process description of industry, it is possible to introduce new technologies to determine, based on economic and performance data, how rapidly these new technologies will penetrate the market (Hyman and Roop, 1996). As these new technologies penetrate the market, energy savings and, possibly, emissions reductions occur that can be tracked with the model as well. This report documents the use of ITEMS to investigate the impact of three new technologies under development with funding from the Department of Energy's Office of Industrial Technologies (OIT), that apply to the iron and steel industry. While the results of this application are interesting, this exercise points out how important it is to understand how the technologies work and how they make a difference. This report shows that ITEMS can be a useful tool in estimating market penetration of new technologies and the resulting energy savings, but these results are only as reliable as the data. If the model is to be used to compare technologies, the technical data concerning these technologies must be collected using the same set of assumptions and with the same vision of what characterizes a technology. While an effort has been made to understand how these technologies work, there is no assurance that the data used for this analysis were, indeed, collected using the same vision and the same set of assumptions. The report is organized into five additional sections. The next provides a brief overview of ITEMS and describes how the technical information about OIT projects is used in the model. The third section describes the three technologies that were introduced into ITEMS and reports the relevant data for those projects. The fourth section describes the iron and steel industry, as characterized

  4. Integrating Efficiency of Industry Processes and Practices Alongside Technology Effectiveness in Space Transportation Cost Modeling and Analysis

    NASA Technical Reports Server (NTRS)

    Zapata, Edgar

    2012-01-01

    This paper presents past and current work in dealing with indirect industry and NASA costs when providing cost estimation or analysis for NASA projects and programs. Indirect costs, when defined as those costs in a project removed from the actual hardware or software hands-on labor; makes up most of the costs of today's complex large scale NASA space/industry projects. This appears to be the case across phases from research into development into production and into the operation of the system. Space transportation is the case of interest here. Modeling and cost estimation as a process rather than a product will be emphasized. Analysis as a series of belief systems in play among decision makers and decision factors will also be emphasized to provide context.

  5. Ratepayer-funded energy-efficiency programs in a restructured electricity industry: Issues, options, and unanswered questions

    SciTech Connect

    Eto, J.; Goldman, C.; Kito, S.

    1996-08-01

    Regulated utilities have, in the past, been responsible for {open_quotes}Public purpose{close_quotes} programs that contribute to the general social good, such as energy-efficiency programs. In several states, continuation of these programs has become a critical issue in forging the consensus required to proceed with restructuring. As a result of reviewing the restructuring process in several states, we expect this trend to continue, but do not believe a single, generic approach can or should be defined. Instead, we expect a variety of solutions based on considerations unique to individual states or regions. To help structure these discussions in states struggling with this issue, we pose a series of questions and describe a range of possible answers: (1) We encourage state public utility commissions and legislatures to provide clear guidance on goals. (2) Close attention to the primary objectives for energy efficiency is important because the objectives influence the choices of programs and activities to be supported. (3) We advocate that states adopt a pragmatic approach to resolving the potentially contentious issue of determining whether or not utilities should continue to have primary responsibility for program administration, management, and design. The approach we propose involves assessing a utility`s past performance, its cur-rent commitment to energy-efficiency activities, and the potential conflicts of interest presented, if the utility retains a central role in administering energy-efficiency programs after restructuring. (4) A state should first assess policy options to mitigate adverse incentives and conflicts of interest in the utility before examining the possibility of having a non-utility entity assume responsibility for designing and managing energy-efficiency activities. (5) If a state does pursue non-utility administration for ratepayer-funded energy-efficiency programs, explicit attention must be paid to governance and accountability issues.

  6. Assessment of Energy Efficiency Improvement and CO2 Emission Reduction Potentials in the Iron and Steel Industry in China

    SciTech Connect

    Hasanbeigi, Ali; Morrow, William; Sathaye, Jayant; Masanet, Eric; Xu, Tengfang

    2012-05-15

    China’s annual crude steel production in 2010 was 638.7 Mt accounting for nearly half of the world’s annual crude steel production in the same year. Around 461 TWh of electricity and 14,872 PJ of fuel were consumed to produce this quantity of steel in 2010. We identified and analyzed 23 energy efficiency technologies and measures applicable to the processes in the iron and steel industry. The Conservation Supply Curve (CSC) used in this study is an analytical tool that captures both the engineering and the economic perspectives of energy conservation. Using a bottom-up electricity CSC model, the cumulative cost-effective electricity savings potential for the Chinese iron and steel industry for 2010-2030 is estimated to be 251 TWh, and the total technical electricity saving potential is 416 TWh. The CO2 emissions reduction associated with cost-effective electricity savings is 139 Mt CO2 and the CO2 emission reduction associated with technical electricity saving potential is 237 Mt CO2. The FCSC model for the iron and steel industry shows cumulative cost-effective fuel savings potential of 11,999 PJ, and the total technical fuel saving potential is 12,139. The CO2 emissions reduction associated with cost-effective and technical fuel savings is 1,191 Mt CO2 and 1,205 Mt CO2, respectively. In addition, a sensitivity analysis with respect to the discount rate used is conducted to assess the effect of changes in this parameter on the results. The result of this study gives a comprehensive and easy to understand perspective to the Chinese iron and steel industry and policy makers about the energy efficiency potential and its associated cost.

  7. Collisions of slow electrons with SiF4 and GeF4: Shape resonance, Ramsauer-Townsend minimum, and virtual state

    NASA Astrophysics Data System (ADS)

    Moreira, G. M.; Bettega, M. H. F.

    2016-06-01

    We report calculated elastic integral, differential, and momentum transfer cross sections for electron impact with the tetrahalides SiF4 and GeF4. The cross sections were computed with the Schwinger multichannel method with pseudopotentials in the static-exchange and static-exchange plus polarization approximations and for energies up to 12 eV. Our results are compared with theoretical and experimental results available in the literature, and in general the agreement is quite good. In addition, we report the presence of a Ramsauer-Townsend minimum and of a T2 shape resonance in the integral cross section of SiF4, and the presence of a virtual state and also of a T2 shape resonance in the GeF4 cross section. These features were also observed and discussed by some experiments and calculations.

  8. FIELD TESTS OF INDUSTRIAL STOKER COAL-FIRED BOILERS FOR EMISSIONS CONTROL AND EFFICIENCY IMPROVEMENT--SITE I

    EPA Science Inventory

    The report gives results of field measurements made on a 70,000 lb steam/hr coal-fired overfeed stoker with traveling grate. The effects of various parameters on boiler emissions and efficiency were studied. Parameters include overfire air, excess oxygen, grate heat release, and ...

  9. FIELD TESTS OF INDUSTRIAL STOKER COAL-FIRED BOILERS FOR EMISSIONS CONTROL AND EFFICIENCY IMPROVEMENT - SITE K

    EPA Science Inventory

    The report gives results of field measurements made on a 50,000 lb stream/hr coal-fired overfeed stoker with traveling grate. The effects of various parameters on boiler emissions and efficiency were studied. Parameters include overfire air, excess oxygen, grate heat release, and...

  10. FIELD TESTS OF INDUSTRIAL STOKER COAL-FIRED BOILERS FOR EMISSIONS CONTROL AND EFFICIENCY IMPROVEMENT - SITE D

    EPA Science Inventory

    The report gives results of field measurements made on a 90,000 lb/hr vibrating-grate-stoker boiler. The effect of various parameters on boiler emissions and efficiency was studied. Parameters included overfire air, excess air, boiler load, and fuel properties. Measurements inclu...

  11. FIELD TESTS OF INDUSTRIAL STOKER COAL-FIRED BOILERS FOR EMISSIONS CONTROL AND EFFICIENCY IMPROVEMENT - SITE B

    EPA Science Inventory

    The report gives results of field measurements made on a 200,000 lb/hr spreader stoker boiler. The effect of various parameters on boiler emissions and efficiency was studied. Parameters studied included overfire air, flyash reinjection, excess air, boiler load, and fuel properti...

  12. FIELD TESTS OF INDUSTRIAL STOKER COAL-FIRED BOILERS FOR EMISSIONS CONTROL AND EFFICIENCY IMPROVEMENT - SITE C

    EPA Science Inventory

    The report gives results of field measurements made on a 182,5000 lb/hr spreader stoker boiler. The effect of various parameters on boiler emissions and efficiency was studied. Parameters included overfire air, flyash reinjection, excess air, boiler load, and fuel properties. Mea...

  13. The Energy-Efficient Quarry: Towards improved understanding and optimisation of energy use and minimisation of CO2 generation in the aggregates industry.

    NASA Astrophysics Data System (ADS)

    Hill, Ian; White, Toby; Owen, Sarah

    2014-05-01

    Extraction and processing of rock materials to produce aggregates is carried out at some 20,000 quarries across the EU. All stages of the processing and transport of hard and dense materials inevitably consume high levels of energy and have consequent significant carbon footprints. The FP7 project "the Energy Efficient Quarry" (EE-Quarry) has been addressing this problem and has devised strategies, supported by modelling software, to assist the quarrying industry to assess and optimise its energy use, and to minimise its carbon footprint. Aggregate quarries across Europe vary enormously in the scale of the quarrying operations, the nature of the worked mineral, and the processing to produce a final market product. Nevertheless most quarries involve most or all of a series of essential stages; deposit assessment, drilling and blasting, loading and hauling, and crushing and screening. The process of determining the energy-efficiency of each stage is complex, but is broadly understood in principle and there are numerous sources of information and guidance available in the literature and on-line. More complex still is the interaction between each of these stages. For example, using a little more energy in blasting to increase fragmentation may save much greater energy in later crushing and screening, but also generate more fines material which is discarded as waste and the embedded energy in this material is lost. Thus the calculation of the embedded energy in the waste material becomes an input to the determination of the blasting strategy. Such feedback loops abound in the overall quarry optimisation. The project has involved research and demonstration operations at a number of quarries distributed across Europe carried out by all partners in the EE-Quarry project, working in collaboration with many of the major quarrying companies operating in the EU. The EE-Quarry project is developing a sophisticated modelling tool, the "EE-Quarry Model" available to the quarrying

  14. Technical and economic analysis of the decisions efficiency of system of water vapor condensate collecting and reusing organization in the industrial firm practice

    NASA Astrophysics Data System (ADS)

    Khamidullina, G. R.

    2016-06-01

    We consider the technical and economic aspects of power - and resource-recovery decisions on a steam condensate recycling and effective using of the (residual) afterheat containing in it basing on the specifics of the industrial enterprises. Below there are the results of the technical and economic analysis of the original innovative solution introduced in practice of the organization of system of collecting and recycling using of water vapor condensate in the conditions of high probability of its pollution by hydrocarbon joints and a non-return of condensate to the source. Also we showed here the high efficiency of the proposed solution.

  15. Opportunities to improve energy efficiency and reduce greenhouse gas emissions in the U.S. pulp and paper industry

    SciTech Connect

    Martin, Nathan; Anglani, N.; Einstein, D.; Khrushch, M.; Worrell, E.; Price, L.K.

    2000-07-01

    The pulp and paper industry accounts for over 12% of total manufacturing energy use in the U.S. (U.S. EIA 1997a), contributing 9% to total manufacturing carbon dioxide emissions. In the last twenty-five years primary energy intensity in the pulp and paper industry has declined by an average of 1% per year. However, opportunities still exist to reduce energy use and greenhouse gas emissions in the manufacture of paper in the U.S. This report analyzes the pulp and paper industry (Standard Industrial Code (SIC) 26) and includes a detailed description of the processes involved in the production of paper, providing typical energy use in each process step. We identify over 45 commercially available state-of-the-art technologies and measures to reduce energy use and calculate potential energy savings and carbon dioxide emissions reductions. Given the importance of paper recycling, our analysis examines two cases. Case A identifies potential primary energy savings without accounting for an increase in recycling, while Case B includes increasing paper recycling. In Case B the production volume of pulp is reduced to account for additional pulp recovered from recycling. We use a discount rate of 30% throughout our analysis to reflect the investment decisions taken in a business context. Our Case A results indicate that a total technical potential primary energy savings of 31% (1013 PJ) exists. For case A we identified a cost-effective savings potential of 16% (533 PJ). Carbon dioxide emission reductions from the energy savings in Case A are 25% (7.6 MtC) and 14% (4.4 MtC) for technical and cost-effective potential, respectively. When recycling is included in Case B, overall technical potential energy savings increase to 37% (1215 PJ) while cost-effective energy savings potential is 16%. Increasing paper recycling to high levels (Case B) is nearly cost-effective assuming a cut-off for cost-effectiveness of a simple payback period of 3 years. If this measure is included, then

  16. Opportunities to improve energy efficiency and reduce greenhouse gas emissions in the US pulp and paper industry

    SciTech Connect

    Martin, Nathan; Anglani, N.; Einstein, D.; Khrushch, M.; Worrell, E.; Price, L.K.

    2000-07-01

    The pulp and paper industry accounts for over 12% of total manufacturing energy use in the US (US EIA 1997a), contributing 9% to total manufacturing carbon dioxide emissions. In the last twenty-five years primary energy intensity in the pulp and paper industry has declined by an average of 1% per year. However, opportunities still exist to reduce energy use and greenhouse gas emissions in the manufacture of paper in the US This report analyzes the pulp and paper industry (Standard Industrial Code (SIC) 26) and includes a detailed description of the processes involved in the production of paper, providing typical energy use in each process step. We identify over 45 commercially available state-of-the-art technologies and measures to reduce energy use and calculate potential energy savings and carbon dioxide emissions reductions. Given the importance of paper recycling, our analysis examines two cases. Case A identifies potential primary energy savings without accounting for an increase in recycling, while Case B includes increasing paper recycling. In Case B the production volume of pulp is reduced to account for additional pulp recovered from recycling. We use a discount rate of 30% throughout our analysis to reflect the investment decisions taken in a business context. Our Case A results indicate that a total technical potential primary energy savings of 31% (1013 PJ) exists. For case A we identified a cost-effective savings potential of 16% (533 PJ). Carbon dioxide emission reductions from the energy savings in Case A are 25% (7.6 MtC) and 14% (4.4 MtC) for technical and cost-effective potential, respectively. When recycling is included in Case B, overall technical potential energy savings increase to 37% (1215 PJ) while cost-effective energy savings potential is 16%. Increasing paper recycling to high levels (Case B) is nearly cost-effective assuming a cut-off for cost-effectiveness of a simple payback period of 3 years. If this measure is included, then the

  17. Comparing different energy partitions at scission used in prompt emission model codes GEF and Point-by-Point

    NASA Astrophysics Data System (ADS)

    Tudora, A.; Hambsch, F.-J.; Visan, I.; Giubega, G.

    2015-08-01

    Different methods to partition the total excitation energy (TXE) of fully accelerated fragments, presently used in prompt emission calculations include different assumptions about what is happening at scission. In fact the energy partition takes place at scission or even before scission, depending on the physical assumptions supporting the models used in different methods of TXE partition. The paper discusses two TXE partition methods in which the amount of energy to be shared (at scission and before scission, respectively) is very different. These methods (based on different principles and physical considerations) are: A. The method used in the Point-by-Point (PbP) treatment of prompt emission in which the available excitation energy at scission is shared between complementary nascent fragments. The amount of energy to be shared is sufficiently high to consider the nascent fragments in the Fermi-gas regime of the level density. B. The method used in the GEF code, in which the intrinsic energy before scission is shared between pre-nascent fragments according to the "energy sorting mechanism". This sorting mechanism is based on the assumption of level densities in the constant temperature regime, only. This is supported by the low amount of the shared intrinsic energy in the case of thermal and low energy neutron induced fission. Taking into account that the principles and physical considerations of any TXE partition method are independent on the way to treat the prompt emission (i.e. deterministically as in the PbP model or probabilistically by Monte-Carlo as in the code GEF) the methods A and B are applied to the same fission fragment range (built as in the PbP treatment). Extreme hypotheses are made for the fragment level densities on which the partitions are based (only in the Fermi-gas regime or only in the constant temperature regime). The results are compared with the energy partition obtained with fragment level densities described by the composite Gilbert

  18. The 5f2-->5f16d1 absorption spectrum of Cs2GeF6:U4+ crystals: A quantum chemical and experimental study.

    PubMed

    Ordejón, Belén; Karbowiak, Miroslaw; Seijo, Luis; Barandiarán, Zoila

    2006-08-21

    Single crystals of U(4+)-doped Cs2GeF6 with 1% U4+ concentration have been obtained by the modified Bridgman-Stockbarger method in spite of the large difference in ionic radii between Ge4+ and U4+ in octahedral coordination. Their UV absorption spectrum has been recorded at 7 K, between 190 and 350 nm; it consists of a first broad and intense band peaking at about 38,000 cm(-1) followed by a number of broad bands of lower intensity from 39,000 to 45,000 cm(-1). None of the bands observed shows appreciable fine vibronic structure, so that the energies of experimental electronic origins cannot be deduced and the assignment of the experimental spectrum using empirical methods based on crystal field theory cannot be attempted. Alternatively, the profile of the absorption spectrum has been obtained theoretically using the U-F bond lengths and totally symmetric vibrational frequencies of the ground 5f2 - 1A(1g) and 5f16d(t(2g))1 - iT(1u) excited states, their energy differences, and their corresponding electric dipole transition moments calculated using the relativistic ab initio model potential embedded cluster method. The calculations suggest that the observed bands are associated with the lowest five 5f2 - 1A(1g)-->5f16d(t(2g))1 - iT(1u) (i = 1-5) dipole allowed electronic origins and their vibrational progressions. In particular, the first broad and intense band peaking at about 38,000 cm(-1) can be safely assigned to the 0-0 and 0-1 members of the a(1g) progression of the 5f2 - 1A(1g)-->5f16d(t(2g))1 - 1T(1u) electronic origin. The electronic structure of all the states with main configurational character 5f16d(t(2g))1 has been calculated as well. The results show that the lowest crystal level of this manifold is 5f16d(t(2g))1 - 1E(u) and lies about 6200 cm(-1) above the 5f2 level closest in energy, which amounts to some 11 vibrational quanta. This large energy gap could result in low nonradiative decay and efficient UV emission, which suggest the interest of

  19. A lesson from Japan: research and development efficiency is a key element of pharmaceutical industry consolidation process.

    PubMed

    Shimura, Hirohisa; Masuda, Sachiko; Kimura, Hiromichi

    2014-02-01

    Scholarly attention to pharmaceutical companies' ability to sustain research and development (R&D) productivity has increased as they increasingly handle business challenges. Furthermore, the deterioration of R&D productivity has long been considered a major cause of mergers and acquisitions (M&As). This study attempts to investigate quantitatively the possible causes of the deterioration and the relationship between the deterioration and M&As by examining the Japanese pharmaceutical industry. Japan from 1980 to 1997 is an ideal case because of the availability of official data, but more importantly the significant changes in its business environment at the time. Using the Malmquist Index and data envelopment analysis, we measured the deterioration of R&D productivity from 1980 to 1997 based on a sample of 15 Japanese companies. Two lessons can be learned from Japan's case. First, to sustain R&D productivity over the long term, companies should use licensing activities and focus on the dominant therapeutic franchises. Second, if a company fails significantly to catch up with the benchmark, it is likely to pursue an M&A or seek an alternative way to improve R&D productivity. These findings appear similar to the current situation of the global pharmaceutical industry, although Japan pursued more licensing activities than M&A to improve R&D productivity. PMID:24647159

  20. A non-typical sequence of phase transitions in (NH4)3GeF7: optical and structural characterization.

    PubMed

    Mel'nikova, S V; Molokeev, M S; Laptash, N M; Misyul, S V

    2016-03-28

    Single crystals of germanium double salt (NH4)3GeF7 = (NH4)2GeF6·NH4F = (NH4)3[GeF6]F were grown and studied by the methods of polarization optics and X-ray diffraction. The birefringence Δn = (no - ne), the rotation angle of the optical indicatrix ϕ(T) and unit cell parameters were measured in the temperature range 100-400 K. Three structural phase transitions were found at the temperatures: T1↓ = 279.2 K (T1↑ = 279.4 K), T2↑ = 270 K (T2↓ = 268.9 K), T3↓ = 218 K (T3↑ = 227 K). An unusual sequence of symmetry transformations with temperature change was established: P4/mbm (Z = 2) (G1) ↔ Pbam (Z = 4) (G2) ↔ P21/c (Z = 4) (G3) ↔ Pa3[combining macron] (Z = 8) (G4). The crystal structures of different phases were determined. The experimental data were additionally interpreted by a group-theoretical analysis of the complete condensate of order parameters taking into account the critical and noncritical atomic displacements. Strengthening of the N-HF hydrogen bonds can be a driving force of the observed phase transitions. PMID:26903439

  1. Interaction between the Type III Effector VopO and GEF-H1 Activates the RhoA-ROCK Pathway

    PubMed Central

    Hiyoshi, Hirotaka; Okada, Ryu; Matsuda, Shigeaki; Gotoh, Kazuyoshi; Akeda, Yukihiro; Iida, Tetsuya; Kodama, Toshio

    2015-01-01

    Vibrio parahaemolyticus is an important pathogen that causes food-borne gastroenteritis in humans. The type III secretion system encoded on chromosome 2 (T3SS2) plays a critical role in the enterotoxic activity of V. parahaemolyticus. Previous studies have demonstrated that T3SS2 induces actin stress fibers in various epithelial cell lines during infection. This stress fiber formation is strongly related to pathogenicity, but the mechanisms that underlie T3SS2-dependent actin stress fiber formation and the main effector have not been elucidated. In this study, we identified VopO as a critical T3SS2 effector protein that activates the RhoA-ROCK pathway, which is an essential pathway for the induction of the T3SS2-dependent stress fiber formation. We also determined that GEF-H1, a RhoA guanine nucleotide exchange factor (GEF), directly binds VopO and is necessary for T3SS2-dependent stress fiber formation. The GEF-H1-binding activity of VopO via an alpha helix region correlated well with its stress fiber-inducing capacity. Furthermore, we showed that VopO is involved in the T3SS2-dependent disruption of the epithelial barrier. Thus, VopO hijacks the RhoA-ROCK pathway in a different manner compared with previously reported bacterial toxins and effectors that modulate the Rho GTPase signaling pathway. PMID:25738744

  2. Snf1/AMP-activated protein kinase activates Arf3p to promote invasive yeast growth via a non-canonical GEF domain

    PubMed Central

    Hsu, Jia-Wei; Chen, Kuan-Jung; Lee, Fang-Jen S.

    2015-01-01

    Active GTP-bound Arf GTPases promote eukaryotic cell membrane trafficking and cytoskeletal remodelling. Arf activation is accelerated by guanine nucleotide-exchange factors (GEFs) using the critical catalytic glutamate in all known Sec7 domain sequences. Yeast Arf3p, a homologue of mammalian Arf6, is required for yeast invasive responses to glucose depletion. Here we identify Snf1p as a GEF that activates Arf3p when energy is limited. SNF1 is the yeast homologue of AMP-activated protein kinase (AMPK), which is a key regulator of cellular energy homeostasis. As activation of Arf3p does not depend on the Snf1p kinase domain, assay of regulatory domain fragments yield evidence that the C-terminal hydrophobic α-helix core of Snf1p is a non-canonical GEF for Arf3p activation. Thus, our study reveals a novel mechanism for regulating cellular responses to energy deprivation, in particular invasive cell growth, through direct Arf activation by Snf1/AMPK. PMID:26198097

  3. Identification and comparative analysis of novel alternatively spliced transcripts of RhoGEF domain encoding gene in C. elegans and C. briggsae

    PubMed Central

    Kashyap, Luv; Tabish, Mohammad; Ganesh, Subramaniam; Dubey, Deepti

    2007-01-01

    Y95B8A.12 gene of C. elegans encodes RhoGEF domain, which is a novel module in the Guanine nucleotide exchange factors (GEFs). Alternative splicing increases transcriptome and proteome diversification. Y95B8A.12 gene has two reported alternatively spliced transcripts by the C. elegans genome sequencing consortium. In the work presented here, we report the presence of four new spliced transcripts of Y95B8A.12 arising as a result of alternative splicing in the pre-mRNA encoded by Y95B8A.12 gene. Our methodology involved the use of various gene or exon finding programmes and several other bioinformatics tools followed by experimental validation. We have also studied alternative splicing pattern in RhoGEF domain encoding orthologues gene from C. briggsae and have obtained very similar results. These new unreported spliced transcripts, which were not detected through conventional approaches, not only point towards the extent of alternative splicing in C. elegans genes but also emphasize towards the need of analyzing genome data using a combinations of bioinformatics tools to delineate all possible gene products. PMID:18188419

  4. Energy Efficiency Improvement and Cost Saving Opportunities for the Vehicle Assembly Industry: An ENERGY STAR Guide for Energy and Plant Managers

    SciTech Connect

    Galitsky, Christina; Galitsky, Christina; Worrell, Ernst

    2008-01-01

    The motor vehicle industry in the U.S. spends about $3.6 billion on energy annually. In this report, we focus on auto assembly plants. In the U.S., over 70 assembly plants currently produce 13 million cars and trucks each year. In assembly plants, energy expenditures is a relatively small cost factor in the total production process. Still, as manufacturers face an increasingly competitive environment, energy efficiency improvements can provide a means to reduce costs without negatively affecting the yield or the quality of the product. In addition, reducing energy costs reduces the unpredictability associated with variable energy prices in today?s marketplace, which could negatively affect predictable earnings, an important element for publicly-traded companies such as those in the motor vehicle industry. In this report, we first present a summary of the motor vehicle assembly process and energy use. This is followed by a discussion of energy efficiency opportunities available for assembly plants. Where available, we provide specific primary energy savings for each energy efficiency measure based on case studies, as well as references to technical literature. If available, we have listed costs and typical payback periods. We include experiences of assembly plants worldwide with energy efficiency measures reviewed in the report. Our findings suggest that although most motor vehicle companies in the U.S. have energy management teams or programs, there are still opportunities available at individual plants to reduce energy consumption cost effectively. Further research on the economics of the measures for individual assembly plants, as part of an energy management program, is needed to assess the potential impact of selected technologies at these plants.

  5. Continuous Space Education System and its Role in Increasing Efficiency of Engineering Staff Training for Ukraine Space Rocket Industry

    NASA Astrophysics Data System (ADS)

    Novykov, O.; Perlik, V.; Polyakov, N.; Khytorniy, V.

    2009-01-01

    Adjustment to new economical and social conditions in Ukraine, being a space-faring country, requires a new concept in improving efficiency of engineering education to provide rocket and space field with highly qualified engineers and scientists. General strategy to solve this task is to combine the efforts of the secondary schools, academic institutions, R&D institutes and production enterprises in order to educe gifted youth as early as possible and to train them into rocket and space field specialists following the scheme of continuous education: school - institution of higher education - enterprise. This report analyzes the 20-year experience of Dniepropetrovsk State University, Yuzhnoye State Design Office and Ukraine's National Center for the Aerospace Education of Youth in their joint efforts to organize the system of continuous education and how it managed to enhance the training efficiency of the engineering skills.

  6. High-Tech Means High-Efficiency: The Business Case for EnergyManagement in High-Tech Industries

    SciTech Connect

    Shanshoian, Gary; Blazek, Michele; Naughton, Phil; Seese, RobertS.; Mills, Evan; Tschudi, William

    2005-11-15

    In the race to apply new technologies in ''high-tech'' facilities such as data centers, laboratories, and clean rooms, much emphasis has been placed on improving service, building capacity, and increasing speed. These facilities are socially and economically important, as part of the critical infrastructure for pharmaceuticals,electronics, communications, and many other sectors. With a singular focus on throughput, some important design issues can be overlooked, such as the energy efficiency of individual equipment (e.g., lasers, routers and switches) as well as the integration of high-tech equipment into the power distribution system and the building envelope. Among technology-based businesses, improving energy efficiency presents an often untapped opportunity to increase profits, enhance process control,maximize asset value, improve the work place environment, and manage a variety of business risks. Oddly enough, the adoption of energy efficiency improvements in this sector lags behind many others. As a result, millions of dollars are left on the table with each year ofoperation.

  7. Molecular characterization of a thermophilic endo-polygalacturonase from Thielavia arenaria XZ7 with high catalytic efficiency and application potential in the food and feed industries.

    PubMed

    Tu, Tao; Meng, Kun; Huang, Huoqing; Luo, Huiying; Bai, Yingguo; Ma, Rui; Su, Xiaoyun; Shi, Pengjun; Yang, Peilong; Wang, Yaru; Yao, Bin

    2014-12-31

    Thermophilic endo-polygalacturonases with high catalytic efficiency are of great interest in the food and feed industries. This study identified an endo-polygalacturonase gene (pg7fn) of glycoside hydrolase family 28 in the thermophilic fungus Thielavia arenaria XZ7. Recombinant PG7fn produced in Pichia pastoris is distinguished from other enzyme counterparts by its high functional temperature (60 °C) and specific activity (34382 ± 351 U/mg toward polygalacturonic acid). The enzyme exhibited good pH stability (pH 3.0-8.0) and resistance to pepsin and trypsin digestion and had a significant effect on disaggregation of soybean meal. Addition of 1 U/g PG7fn increased the pectin bioavailability by 19.33%. The excellent properties described above make PG7fn valuable for applications in the food and feed industries. Furthermore, a comparative study showed that N-glycosylation improved the thermostability and catalytic efficiency of PG7fn. PMID:25494480

  8. The RhoA GEF, LARG, mediates ICAM-1-dependent mechanotransduction in endothelial cells to stimulate transendothelial migration

    PubMed Central

    Lessey-Morillon, Elizabeth C.; Osborne, Lukas D.; Monaghan-Benson, Elizabeth; Guilluy, Christophe; O’Brien, E. Timothy; Superfine, Richard; Burridge, Keith

    2014-01-01

    RhoA-mediated cytoskeletal rearrangements in endothelial cells (ECs) play an active role in leukocyte transendothelial cell migration (TEM), a normal physiological process in which leukocytes cross the endothelium to enter the underlying tissue. While much has been learned about RhoA signaling pathways downstream from ICAM-1 in ECs, little is known about the consequences of the tractional forces that leukocytes generate on ECs as they migrate over the surface before TEM. We have found that after applying mechanical forces to ICAM-1 clusters, there is an increase in cellular stiffening and enhanced RhoA signaling compared to ICAM-1 clustering alone. We have identified that the RhoA GEF LARG/ARHGEF12 acts downstream of clustered ICAM-1 to increase RhoA activity and that this pathway is further enhanced by mechanical force on ICAM-1. Depletion of LARG decreases leukocyte crawling and inhibits TEM. This is the first report of endothelial LARG regulating leukocyte behavior and EC stiffening in response to tractional forces generated by leukocytes. PMID:24585879

  9. DOE/Industrial Technologies Program DOE Award Number DE-FG36-05GO15099 Plant Wide Energy Efficiency Assessment Pilgrims Pride Corporation – Mt Pleasant Facility

    SciTech Connect

    Paper, Riyaz; Dooley, Bill; Turpish, William J; Symonds, Mark; Carswell, Needham

    2007-04-13

    The U. S. Department of Energy’s (DOE) Industrial Technologies Program (ITP), through Oak Ridge National Laboratory, is supporting plant wide energy efficiency assessments that will lead to substantial improvements in industrial efficiency, waste reduction, productivity, and global competitiveness in industries identified in ITP’s Industries of the Future. The stated goal of the assessments is to develop a comprehensive strategy at manufacturing locations that will significantly increase plant productivity, profitability, and energy efficiency, and reduce environmental emissions. ITP awarded a contract to Pilgrim’s Pride Corporation to conduct a plant wide energy efficiency assessment for their Mt Pleasant Facility in Mt Pleasant, Texas. Pilgrim’s Pride Corporation is the largest poultry company in the U.S. and Mexico producing nearly 9 billion pounds of poultry per year. Pilgrim's Pride products are sold to foodservice, retail and frozen entrée customers. Pilgrim's Pride owns and operates 37 chicken processing plants (34 in the U.S. and three in Mexico), 12 prepared foods plants and one turkey processing plant. Thirty-five feed mills and 49 hatcheries support these plants. Pilgrim's Pride is ranked number 382 on 2006's FORTUNE 500 list and net sales were $7.4 billion. In Mt. Pleasant, Texas, Pilgrim's Pride operates one of the largest prepared foods plants in the United States, with the capability of producing 2,000 different products and the capacity to turn out more than 7 million pounds of finished goods per week. The facility is divided into distinct departments: East Kill, West Kill, Prepared Foods, Protein Conversion, Wastewater Treatment, and Truck Shop. Facility processes include killing, eviscerating, refrigeration, baking, frying, and protein conversion. Pilgrim’s Pride formed a team to complete the plant wide energy efficiency assessment. The scope of work for this project was to: provide the analysis of departmental energy use, identify

  10. A highly efficient polyampholyte hydrogel sorbent based fixed-bed process for heavy metal removal in actual industrial effluent.

    PubMed

    Zhou, Guiyin; Luo, Jinming; Liu, Chengbin; Chu, Lin; Ma, Jianhong; Tang, Yanhong; Zeng, Zebing; Luo, Shenglian

    2016-02-01

    High sorption capacity, high sorption rate, and fast separation and regeneration for qualified sorbents used in removing heavy metals from wastewater are urgently needed. In this study, a polyampholyte hydrogel was well designed and prepared via a simple radical polymerization procedure. Due to the remarkable mechanical strength, the three-dimensional polyampholyte hydrogel could be fast separated, easily regenerated and highly reused. The sorption capacities were as high as 216.1 mg/g for Pb(II) and 153.8 mg/g for Cd(II) owing to the existence of the large number of active groups. The adsorption could be conducted in a wide pH range of 3-6 and the equilibrium fast reached in 30 min due to its excellent water penetration for highly accessible to metal ions. The fixed-bed column sorption results indicated that the polyampholyte hydrogel was particularly effective in removing Pb(II) and Cd(II) from actual industrial effluent to meet the regulatory requirements. The treatment volumes of actual smelting effluent using one fixed bed column were as high as 684 bed volumes (BV) (7736 mL) for Pb(II) and 200 BV (2262 mL) for Cd(II). Furthermore, the treatment volumes of actual smelting effluent using tandem three columns reached 924 BV (31,351 mL) for Pb(II) and 250 BV (8483 mL) for Cd(II), producing only 4 BV (136 mL) eluent. Compared with the traditional high density slurry (HDS) process with large amount of sludge, the proposed process would be expected to produce only a small amount of sludge. When the treatment volume was controlled below 209.3 BV (7103 mL), all metal ions in the actual industrial effluent could be effectively removed (<0.01 mg/L). This wok develops a highly practical process based on polyampholyte hydrogel sorbents for the removal of heavy metal ions from practical wastewater. PMID:26650450

  11. Graphene oxide based CdSe photocatalysts: Synthesis, characterization and comparative photocatalytic efficiency of rhodamine B and industrial dye

    SciTech Connect

    Ghosh, Trisha; Lee, Jeong-Ho; Meng, Ze-Da; Ullah, Kefayat; Park, Chong-Yeon; Nikam, Vikram; Oh, Won-Chun

    2013-03-15

    Highlights: ► CdSe–graphene is synthesized by hydrothermal method. ► Three molar solutions of CdSe were used making three different composites. ► RhB and Texbrite MST-L were used as sample dye solutions. ► Texbrite MST-L is photo degraded in visible light. ► UV-spectroscopic analysis was done to measure degradation. - Abstract: CdSe–graphene composites were prepared using simple “hydrothermal method” where the graphene surface was modified using different molar solutions of cadmium selenide (CdSe) in aqueous media. The characterization of CdSe–graphene composites were studied by X-ray diffraction (XRD), energy dispersive X-ray (EDX), scanning electron microscope (SEM), and with transmission electron microscope (TEM). The catalytic activities of CdSe-composites were evaluated by degradation of rhodamine B (RhB) and commercial industrial dye “Texbrite MST-L (TXT-MST)” with fixed concentration. The degradation was observed by the decrease in the absorbance peak studied by UV spectrophotometer. The decrease in the dye concentration indicated catalytic degradation effect by CdSe–graphene composites.

  12. Central role of the exchange factor GEF-H1 in TNF-α–induced sequential activation of Rac, ADAM17/TACE, and RhoA in tubular epithelial cells

    PubMed Central

    Waheed, Faiza; Dan, Qinghong; Amoozadeh, Yasaman; Zhang, Yuqian; Tanimura, Susumu; Speight, Pam; Kapus, András; Szászi, Katalin

    2013-01-01

    Transactivation of the epidermal growth factor receptor (EGFR) by tumor necrosis factor-α (TNF-α) is a key step in mediating RhoA activation and cytoskeleton and junction remodeling in the tubular epithelium. In this study we explore the mechanisms underlying TNF-α–induced EGFR activation. We show that TNF-α stimulates the TNF-α convertase enzyme (TACE/a disintegrin and metalloproteinase-17), leading to activation of the EGFR/ERK pathway. TACE activation requires the mitogen-activated protein kinase p38, which is activated through the small GTPase Rac. TNF-α stimulates both Rac and RhoA through the guanine nucleotide exchange factor (GEF)-H1 but by different mechanisms. EGFR- and ERK-dependent phosphorylation at the T678 site of GEF-H1 is a prerequisite for RhoA activation only, whereas both Rac and RhoA activation require GEF-H1 phosphorylation on S885. Of interest, GEF-H1-mediated Rac activation is upstream from the TACE/EGFR/ERK pathway and regulates T678 phosphorylation. We also show that TNF-α enhances epithelial wound healing through TACE, ERK, and GEF-H1. Taken together, our findings can explain the mechanisms leading to hierarchical activation of Rac and RhoA by TNF-α through a single GEF. This mechanism could coordinate GEF functions and fine-tune Rac and RhoA activation in epithelial cells, thereby promoting complex functions such as sheet migration. PMID:23389627

  13. Development of Bottom-up Representation of Industrial Energy Efficiency Technologies in Integrated Assessment Models for the Iron and Steel Sector

    SciTech Connect

    Xu, T.T.; Sathaye, J.; Galitsky, C.

    2010-09-30

    Adoption of efficient end-use technologies is one of the key measures for reducing greenhouse gas (GHG) emissions. With the working of energy programs and policies on carbon regulation, how to effectively analyze and manage the costs associated with GHG reductions become extremely important for the industry and policy makers around the world. Energy-climate (EC) models are often used for analyzing the costs of reducing GHG emissions (e.g., carbon emission) for various emission-reduction measures, because an accurate estimation of these costs is critical for identifying and choosing optimal emission reduction measures, and for developing related policy options to accelerate market adoption and technology implementation. However, accuracies of assessing of GHG-emission reduction costs by taking into account the adoption of energy efficiency technologies will depend on how well these end-use technologies are represented in integrated assessment models (IAM) and other energy-climate models. In this report, we first conduct brief overview on different representations of end-use technologies (mitigation measures) in various energy-climate models, followed by problem statements, and a description of the basic concepts of quantifying the cost of conserved energy including integrating non-regrets options. A non-regrets option is defined as a GHG reduction option that is cost effective, without considering their additional benefits related to reducing GHG emissions. Based upon these, we develop information on costs of mitigation measures and technological change. These serve as the basis for collating the data on energy savings and costs for their future use in integrated assessment models. In addition to descriptions of the iron and steel making processes, and the mitigation measures identified in this study, the report includes tabulated databases on costs of measure implementation, energy savings, carbon-emission reduction, and lifetimes. The cost curve data on mitigation

  14. Development and testing of a high efficiency advanced coal combustor: Phase 3 industrial boiler retrofit. Final report

    SciTech Connect

    Patel, R.L.; Thornock, D.E.; Miller, B.G.; Scaroni, A.W.; McGowan, J.G.

    1998-03-01

    Economics and/or political intervention may one day dictate the conversion from oil or natural gas to coal in boilers that were originally designed to burn oil or gas. In recognition of this future possibility the US Department of Energy, Federal Energy Technical Center (DOE-FETC) supported a program led by ABB Power Plant Laboratories with support from the Energy and Fuels Research Center of Penn State University with the goal of demonstrating the technical and economic feasibility of retrofitting a gas/oil designed boiler to burn micronized coal. In support of the overall goal the following specific objectives were targeted: develop a coal handling/preparation system that can meet the technical and operational requirements for retrofitting microfine coal on a boiler designed for burning oil or natural gas; maintain boiler thermal performance in accordance with specifications when burning oil or natural gas; maintain NOx emissions at or below 0.6 lb NO{sub 2} per million Btu; achieve combustion efficiencies of 98% or higher; and determine economic payback periods as a function of key variables.

  15. Development and testing of a high efficiency advanced coal combustor Phase III industrial boiler retrofit. Quarterly technical progress report, July 1, 1995--September 30, 1995 No. 16

    SciTech Connect

    Borio, R.W.

    1995-12-15

    The objective of this project is to retrofit a burner, capable of firing microfine coal, to a standard gas/oil designed industrial boiler to assess the technical and economic viability of displacing premium fuels with microfine coal. This report documents the technical aspects of this project during the sixteenth quarter (July `95 through September `95) of the program. The overall program has consisted of five major tasks: (1) A review of current state-of-the-art coal firing system components. (2) Design and experimental testing of a prototype HEACC (High Efficiency Advanced Coal Combustor) burner. (3) Installation and testing of a prototype HEACC system in a commercial retrofit application. (4) Economics evaluation of the HEACC concept for retrofit applications. (5) Long term demonstration under commercial user demand conditions.

  16. Final Assessment: U.S. Virgin Islands Industrial Development Park and Adjacent Facilities Energy-Efficiency and Micro-Grid Infrastructure

    SciTech Connect

    Petersen, Joseph M.; Boyd, Paul A.; Dahowski, Robert T.; Parker, Graham B.

    2015-12-31

    The purpose of this assessment was to undertake an assessment and analysis of cost-effective options for energy-efficiency improvements and the deployment of a micro-grid to increase the energy resilience at the U.S. Virgin Islands Industrial Development Park (IDP) and adjacent facilities in St. Croix, Virgin Islands. The Economic Development Authority sought assistance from the U.S. Department of Energy to undertake this assessment undertaken by Pacific Northwest National Laboratory. The assessment included 18 buildings plus the perimeter security lighting at the Virgin Islands Bureau of Correctional Facility, four buildings plus exterior lighting at the IDP, and five buildings (one of which is to be constructed) at the Virgin Islands Police Department for a total of 27 buildings with a total of nearly 323,000 square feet.

  17. Direct interaction of insulin-like growth factor-1 receptor with leukemia-associated RhoGEF

    PubMed Central

    Taya, Shinichiro; Inagaki, Naoyuki; Sengiku, Hiroaki; Makino, Hiroshi; Iwamatsu, Akihiro; Urakawa, Itaru; Nagao, Kenji; Kataoka, Shiro; Kaibuchi, Kozo

    2001-01-01

    Insulin-like growth factor (IGF)-1 plays crucial roles in growth control and rearrangements of the cytoskeleton. IGF-1 binds to the IGF-1 receptor and thereby induces the autophosphorylation of this receptor at its tyrosine residues. The phosphorylation of the IGF-1 receptor is thought to initiate a cascade of events. Although various signaling molecules have been identified, they appear to interact with the tyrosine-phosphorylated IGF-1 receptor. Here, we identified leukemia-associated Rho guanine nucleotide exchange factor (GEF) (LARG), which contains the PSD-95/Dlg/ZO-1 (PDZ), regulator of G protein signaling (RGS), Dbl homology, and pleckstrin homology domains, as a nonphosphorylated IGF-1 receptor-interacting molecule. LARG formed a complex with the IGF-1 receptor in vivo, and the PDZ domain of LARG interacted directly with the COOH-terminal domain of IGF-1 receptor in vitro. LARG had an exchange activity for Rho in vitro and induced the formation of stress fibers in NIH 3T3 fibroblasts. When MDCKII epithelial cells were treated with IGF-1, Rho and its effector Rho-associated kinase (Rho-kinase) were activated and actin stress fibers were enhanced. Furthermore, the IGF-1–induced Rho-kinase activation and the enhancement of stress fibers were inhibited by ectopic expression of the PDZ and RGS domains of LARG. Taken together, these results indicate that IGF-1 activates the Rho/Rho-kinase pathway via a LARG/IGF-1 receptor complex and thereby regulates cytoskeletal rearrangements. PMID:11724822

  18. Designing, construction, assessment, and efficiency of local exhaust ventilation in controlling crystalline silica dust and particles, and formaldehyde in a foundry industry plant.

    PubMed

    Morteza, Mortezavi Mehrizi; Hossein, Kakooi; Amirhossein, Matin; Naser, Hasheminegad; Gholamhossein, Halvani; Hossein, Fallah

    2013-01-01

    The purpose of the present study was to design and assess the efficiency of a local exhaust ventilation system used in a foundry operation to control inhalable dust and particles, microcrystal particles, and noxious gases and vapours affecting workers during the foundry process. It was designed based on recommendations from the American Conference of Governmental Industrial Hygiene. After designing a local exhaust ventilation system (LEV), we prepared and submitted the implementation plan to the manufacturer. High concentrations of crystalline silica dust and formaldehyde, which are common toxic air pollutants in foundries, were ultimately measured as an indicator for studying the efficiency of this system in controlling inhalable dust and particles as well as other air pollutants. The level of occupational exposure to silica and formaldehyde as major air pollutants was assessed in two modes: first, when the LEV was on, and second, when it was off. Air samples from the exposure area were obtained using a personal sampling pump and analysed using the No. 7601 method for crystal silica and the No. 2541 method for formaldehyde of the National Institute for Occupational Safety and Health (NIOSH). Silica and formaldehyde concentrations were determined by visible absorption spectrophotometry and gas chromatography. The results showed that local exhaust ventilation was successful in preserving the crystal silica particles in the work environment at a level below the NIOSH maximum allowed concentration (0.05 mg m-3). In contrast, formaldehyde exceeded the NIOSH limit (1 ppm or 1.228 mg m-3). PMID:23585164

  19. High efficiency electricity production in the sugar industry of the future: The Pacific International Center for High Technology Research Project (>6MW{sub e})

    SciTech Connect

    Trenka, A.R.

    1995-12-31

    The Pacific International Center for High Technology Research (PICHTR) is presently starting up a 100 tpd bagasse Renugas{reg_sign} gasifier which was developed under license from the Institute of Gas Technology (IGT). For thousands of years, mankind has used biomass for energy, burning it first in campfires. In more modem times, combustion boiler systems were developed such as those fueled by coal. Through inefficient, these systems answered an increasing need for energy brought on by the industrial revolution. Yesterday`s systems are being replaced with more efficient methods of energy conversion and extraction. Recognizing the untapped potential for biomass power to provide clean and efficient energy, the U.S. Department of Energy established the National Biomass Power Program in 1991. The State of Hawaii Department of Business, Economic Development & Tourism is collaborating in this national program to complement the development of its own sustainable resource program. As a key player in this program, PICHTR will design, construct, and operate a biomass gasification facility that will be the centerpiece of the nation`s biomass gasification technology.

  20. Systematic expression and loss-of-function analysis defines spatially restricted requirements for Drosophila RhoGEFs and RhoGAPs in leg morphogenesis

    PubMed Central

    Greenberg, Lina; Hatini, Victor

    2010-01-01

    The Drosophila leg imaginal disc consists of a peripheral region that contributes to adult body wall, and a central region that forms the leg proper. While the patterning signals and transcription factors that determine the identity of adult structures have been identified, the mechanisms that determine the shape of these structures remain largely unknown. The family of Rho GTPases, which consists of 7 members in flies, modulates cell adhesion, actomyosin contractility, protrusive membrane activity, and cell-matrix adhesion to generate mechanical forces that shape adult structures. The Rho GTPases are ubiquitously expressed and it remains unclear how they orchestrate morphogenetic events. The Rho guanine nucleotide exchange factors (RhoGEFs) and Rho GTPase activating proteins (RhoGAPs), which respectively activate and deactivate corresponding Rho GTPases, have been proposed to regulate the activity of Rho signaling cascades in specific spatiotemporal patterns to orchestrate morphogenetic events. Here we identify restricted expression of 12 of the 20 RhoGEFs and 10 of the 22 Rho RhoGAPs encoded in Drosophila during metamorphosis. Expression of a subset of each family of RhoGTPase regulators was restricted to motile cell populations including tendon, muscle, trachea, and peripodial stalk cells. A second subset was restricted either to all presumptive joints or only to presumptive tarsal joints. Depletion of individual RhoGEFs and RhoGAPs in the epithelium of the disc proper identified several joint-specific genes, which act downstream of segmental patterning signals to control epithelial morphogenesis. Our studies provide a framework with which to understand how Rho signaling cascades orchestrate complex morphogenetic events in multicellular organisms, and evidence that patterning signals regulate these cascades to control apical constriction and epithelial invagination at presumptive joints. PMID:20851182

  1. Nanosecond Dynamics of Gαi1 Bound to Nucleotides or Ric-8A, a Gα Chaperone with GEF Activity.

    PubMed

    Black, Labe A; Thomas, Celestine J; Nix, Gwendolyn N; Terwilliger, Michelle C; Sprang, Stephen R; Ross, J B Alexander

    2016-08-23

    Resistance to Inhibitors of Cholinesterase A (Ric-8A) is a 60-kDa cytosolic protein that has chaperone and guanine nucleotide exchange (GEF) activity toward heterotrimeric G protein α subunits of the i, q, and 12/13 classes, catalyzing the release of GDP from Gα and subsequent binding of GTP. In the absence of GTP or GTP analogs, and subsequent to GDP release, Gα forms a stable nucleotide-free complex with Ric-8A. In this study, time-resolved fluorescence anisotropy measurements were employed to detect local motions of Gαi1 labeled at selected sites with Alexa 488 (C5) fluorescent dye (Ax) in the GDP, GTPγS (collectively, GXP), and Ric-8A-bound states. Sites selected for Alexa 488 (C5) derivatization were in the α-helical domain (residue 106), the α-helical domain-Ras-like domain hinge (residue 63), Switch I (residue 180), Switch II (residue 209), Switch III (residue 238), the α4 helix (residue 305), and at the junction between the purine-binding subsite in the β6-α5 loop and the C-terminal α helix (residue 330). In the GXP-bound states, the Alexa fluorophore reports local motions with correlation times ranging from 1.0 to 1.8 ns. The dynamics at Ax180 is slower in Gαi1•GDP than in Gαi1•GTPγS. The reverse is true at Ax209. The order parameters, S(2), for Alexa probes at switch residues are high (0.78-0.88) in Gαi1•GDP and lower (0.67-0.75) in Gαi1•GTPγS, although in crystal structures, switch segments are more ordered in the latter. Local motions at Ax63, Ax180, Ax209, and Ax330 are all markedly slower (2.3-2.8 ns) in Gαi1:Ric-8A than in Gαi1•GXP, and only modest (± 0.1) differences in S(2) are observed at most sites in Gαi1:Ric-8A relative to Gαi1•GXP. The slow dynamics suggests long-range correlated transitions within an ensemble of states and, particularly in the hinge and switch segments that make direct contact with Ric-8A. Induction of Gαi1 structural heterogeneity by Ric-8A provides a mechanism for nucleotide release

  2. Insights into the Localization and Function of the Membrane Trafficking Regulator GNOM ARF-GEF at the Golgi Apparatus in Arabidopsis[W

    PubMed Central

    Naramoto, Satoshi; Otegui, Marisa S.; Kutsuna, Natsumaro; de Rycke, Riet; Dainobu, Tomoko; Karampelias, Michael; Fujimoto, Masaru; Feraru, Elena; Miki, Daisuke; Fukuda, Hiroo; Nakano, Akihiko; Friml, Jiří

    2014-01-01

    GNOM is one of the most characterized membrane trafficking regulators in plants, with crucial roles in development. GNOM encodes an ARF-guanine nucleotide exchange factor (ARF-GEF) that activates small GTPases of the ARF (ADP ribosylation factor) class to mediate vesicle budding at endomembranes. The crucial role of GNOM in recycling of PIN auxin transporters and other proteins to the plasma membrane was identified in studies using the ARF-GEF inhibitor brefeldin A (BFA). GNOM, the most prominent regulator of recycling in plants, has been proposed to act and localize at so far elusive recycling endosomes. Here, we report the GNOM localization in context of its cellular function in Arabidopsis thaliana. State-of-the-art imaging, pharmacological interference, and ultrastructure analysis show that GNOM predominantly localizes to Golgi apparatus. Super-resolution confocal live imaging microscopy identified GNOM and its closest homolog GNOM-like 1 at distinct subdomains on Golgi cisternae. Short-term BFA treatment stabilizes GNOM at the Golgi apparatus, whereas prolonged exposures results in GNOM translocation to trans-Golgi network (TGN)/early endosomes (EEs). Malformed TGN/EE in gnom mutants suggests a role for GNOM in maintaining TGN/EE function. Our results redefine the subcellular action of GNOM and reevaluate the identity and function of recycling endosomes in plants. PMID:25012191

  3. Purification and crystallization of the catalytic PRONE domain of RopGEF8 and its complex with Rop4 from Arabidopsis thaliana

    SciTech Connect

    Thomas, Christoph; Weyand, Michael; Wittinghofer, Alfred; Berken, Antje

    2006-06-01

    Crystals of the catalytic PRONE domain of the guanine nucleotide exchange factor RopGEF8 and its complex with the Rho-family protein Rop4 from A. thaliana were obtained that diffract to 2.2 and 3.1 Å resolution, respectively. The PRONE domain of the guanine nucleotide exchange factor RopGEF8 (PRONE8) was purified and crystallized free and in complex with the Rho-family protein Rop4 using the hanging-drop vapour-diffusion method. PRONE8 crystals were obtained using NaCl as precipitating agent and belong to the hexagonal space group P6{sub 5}22. Native and anomalous data sets were collected using synchrotron radiation at 100 K to 2.2 and 2.8 Å resolution, respectively. Crystals of the Rop4–PRONE8 complex belonging to space group P6{sub 3} were obtained using Tacsimate and PEG 3350 as precipitating agents and diffracted to 3.1 Å resolution.

  4. The tight-adhesion proteins TadGEF of Bradyrhizobium diazoefficiens USDA 110 are involved in cell adhesion and infectivity on soybean roots.

    PubMed

    Mongiardini, Elías J; Parisi, Gustavo D; Quelas, Juan I; Lodeiro, Aníbal R

    2016-01-01

    Adhesion of symbiotic bacteria to host plants is an essential early step of the infection process that leads to the beneficial interaction. In the Bradyrhizobium diazoefficiens-soybean symbiosis few molecular determinants of adhesion are known. Here we identified the tight-adhesion gene products TadGEF in the open-reading frames blr3941-blr3943 of the B. diazoefficiens USDA 110 complete genomic sequence. Predicted structure of TadG indicates a transmembrane domain and two extracytosolic domains, from which the C-terminal has an integrin fold. TadE and TadF are also predicted as bearing transmembrane segments. Mutants in tadG or the small cluster tadGEF were impaired in adhesion to soybean roots, and the root infection was delayed. However, nodule histology was not compromised by the mutations, indicating that these effects were restricted to the earliest contact of the B. diazoefficiens and root surfaces. Knowledge of preinfection determinants is important for development of inoculants that are applied to soybean crops worldwide. PMID:26686616

  5. TGF-β regulates LARG and GEF-H1 during EMT to affect stiffening response to force and cell invasion

    PubMed Central

    Osborne, Lukas D.; Li, George Z.; How, Tam; O'Brien, E. Tim; Blobe, Gerard C.; Superfine, Richard; Mythreye, Karthikeyan

    2014-01-01

    Recent studies implicate a role for cell mechanics in cancer progression. The epithelial-to-mesenchymal transition (EMT) regulates the detachment of cancer cells from the epithelium and facilitates their invasion into stromal tissue. Although classic EMT hallmarks include loss of cell–cell adhesions, morphology changes, and increased invasion capacity, little is known about the associated mechanical changes. Previously, force application on integrins has been shown to initiate cytoskeletal rearrangements that result in increased cell stiffness and a stiffening response. Here we demonstrate that transforming growth factor β (TGF-β)–induced EMT results in decreased stiffness and loss of the normal stiffening response to force applied on integrins. We find that suppression of the RhoA guanine nucleotide exchange factors (GEFs) LARG and GEF-H1 through TGF-β/ALK5–enhanced proteasomal degradation mediates these changes in cell mechanics and affects EMT-associated invasion. Taken together, our results reveal a functional connection between attenuated stiffness and stiffening response and the increased invasion capacity acquired after TGF-β–induced EMT. PMID:25143398

  6. Preclinical Development of Novel Rac1-GEF Signaling Inhibitors using a Rational Design Approach in Highly Aggressive Breast Cancer Cell Lines

    PubMed Central

    Cardama, Georgina A; Comin, Maria J; Hornos, Leandro; Gonzalez, Nazareno; Defelipe, Lucas; Turjanski, Adrian G; Alonso, Daniel F; Gomez, Daniel E; Menna, Pablo Lorenzano

    2014-01-01

    Rho GTPases play a key role in the regulation of multiple essential cellular processes, including actin dynamics, gene transcription and cell cycle progression. Aberrant activation of Rac1, a member of Rho family of small GTPases, is associated with tumorigenesis, cancer progression, invasion and metastasis. Particularly, Rac1 is overexpressed and hyperactivated in highly aggressive breast cancer. Thus, Rac1 appears to be a promising and relevant target for the development of novel anticancer drugs. We identified the novel Rac1 inhibitor ZINC69391 through a docking-based virtual library screening targeting Rac1 activation by GEFs. This compound was able to block Rac1 interaction with its GEF Tiam1, prevented EGF-induced Rac1 activation and inhibited cell proliferation, cell migration and cell cycle progression in highly aggressive breast cancer cell lines. Moreover, ZINC69391 showed an in vivo antimetastatic effect in a syngeneic animal model. We further developed the novel analog 1A-116 by rational design and showed to be specific and more potent than the parental compound in vitro and interfered Rac1-P-Rex1 interaction. We also showed an enhanced in vivo potency of 1A-116 analog. These results show that we have developed novel Rac1 inhibitors that may be used as a novel anticancer therapy. PMID:24066799

  7. Experiments and Theoretical Data for Studying the Impact of Fission Yield Uncertainties on the Nuclear Fuel Cycle with TALYS/GEF and the Total Monte Carlo Method

    NASA Astrophysics Data System (ADS)

    Pomp, S.; Al-Adili, A.; Alhassan, E.; Gustavsson, C.; Helgesson, P.; Hellesen, C.; Koning, A. J.; Lantz, M.; Österlund, M.; Rochman, D.; Simutkin, V.; Sjöstrand, H.; Solders, A.

    2015-01-01

    We describe the research program of the nuclear reactions research group at Uppsala University concerning experimental and theoretical efforts to quantify and reduce nuclear data uncertainties relevant for the nuclear fuel cycle. We briefly describe the Total Monte Carlo (TMC) methodology and how it can be used to study fuel cycle and accident scenarios, and summarize our relevant experimental activities. Input from the latter is to be used to guide the nuclear models and constrain parameter space for TMC. The TMC method relies on the availability of good nuclear models. For this we use the TALYS code which is currently being extended to include the GEF model for the fission channel. We present results from TALYS-1.6 using different versions of GEF with both default and randomized input parameters and compare calculations with experimental data for 234U(n,f) in the fast energy range. These preliminary studies reveal some systematic differences between experimental data and calculations but give overall good and promising results.

  8. ARF-GEF cytohesin-2/ARNO regulates R-Ras and α5-integrin recycling through an EHD1-positive compartment

    PubMed Central

    Salem, Joseph C.; Reviriego-Mendoza, Marta M.; Santy, Lorraine C.

    2015-01-01

    When expressed in epithelial cells, cytohesin-2/ARNO, a guanine nucleotide exchange factor (GEF) for ARF small GTPases, causes a robust migration response. Recent evidence suggests that cytohesin-2/ARNO acts downstream of small the GTPase R-Ras to promote spreading and migration. We hypothesized that cytohesin-2/ARNO could transmit R-Ras signals by regulating the recycling of R-Ras through ARF activation. We found that Eps15-homology domain 1 (EHD1), a protein that associates with the endocytic recycling compartment (ERC), colocalizes with active R-Ras in transiently expressed HeLa cells. In addition, we show that EHD1-positive recycling endosomes are a novel compartment for cytohesin-2/ARNO. Knockdown or expression of GEF-inactive (E156K) cytohesin-2/ARNO causes R-Ras to accumulate on recycling endosomes containing EHD1 and inhibits cell spreading. E156K-ARNO also causes a reduction in focal adhesion size and number. Finally, we demonstrate that R-Ras/ARNO signaling is required for recycling of α5-integrin and R-Ras to the plasma membrane. These data establish a role for cytohesin-2/ARNO as a regulator of R-Ras and integrin recycling and suggest that ARF-regulated trafficking of R-Ras is required for R-Ras–dependent effects on spreading and adhesion formation. PMID:26378252

  9. Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis.

    PubMed

    Naramoto, Satoshi; Otegui, Marisa S; Kutsuna, Natsumaro; de Rycke, Riet; Dainobu, Tomoko; Karampelias, Michael; Fujimoto, Masaru; Feraru, Elena; Miki, Daisuke; Fukuda, Hiroo; Nakano, Akihiko; Friml, Jiří

    2014-07-01

    GNOM is one of the most characterized membrane trafficking regulators in plants, with crucial roles in development. GNOM encodes an ARF-guanine nucleotide exchange factor (ARF-GEF) that activates small GTPases of the ARF (ADP ribosylation factor) class to mediate vesicle budding at endomembranes. The crucial role of GNOM in recycling of PIN auxin transporters and other proteins to the plasma membrane was identified in studies using the ARF-GEF inhibitor brefeldin A (BFA). GNOM, the most prominent regulator of recycling in plants, has been proposed to act and localize at so far elusive recycling endosomes. Here, we report the GNOM localization in context of its cellular function in Arabidopsis thaliana. State-of-the-art imaging, pharmacological interference, and ultrastructure analysis show that GNOM predominantly localizes to Golgi apparatus. Super-resolution confocal live imaging microscopy identified GNOM and its closest homolog GNOM-like 1 at distinct subdomains on Golgi cisternae. Short-term BFA treatment stabilizes GNOM at the Golgi apparatus, whereas prolonged exposures results in GNOM translocation to trans-Golgi network (TGN)/early endosomes (EEs). Malformed TGN/EE in gnom mutants suggests a role for GNOM in maintaining TGN/EE function. Our results redefine the subcellular action of GNOM and reevaluate the identity and function of recycling endosomes in plants. PMID:25012191

  10. Experiments and Theoretical Data for Studying the Impact of Fission Yield Uncertainties on the Nuclear Fuel Cycle with TALYS/GEF and the Total Monte Carlo Method

    SciTech Connect

    Pomp, S.; Al-Adili, A.; Alhassan, E.; Gustavsson, C.; Helgesson, P.; Hellesen, C.; Koning, A.J.; Lantz, M.; Österlund, M.; Rochman, D.; Simutkin, V.; Sjöstrand, H.; Solders, A.

    2015-01-15

    We describe the research program of the nuclear reactions research group at Uppsala University concerning experimental and theoretical efforts to quantify and reduce nuclear data uncertainties relevant for the nuclear fuel cycle. We briefly describe the Total Monte Carlo (TMC) methodology and how it can be used to study fuel cycle and accident scenarios, and summarize our relevant experimental activities. Input from the latter is to be used to guide the nuclear models and constrain parameter space for TMC. The TMC method relies on the availability of good nuclear models. For this we use the TALYS code which is currently being extended to include the GEF model for the fission channel. We present results from TALYS-1.6 using different versions of GEF with both default and randomized input parameters and compare calculations with experimental data for {sup 234}U(n,f) in the fast energy range. These preliminary studies reveal some systematic differences between experimental data and calculations but give overall good and promising results.

  11. Impact of surface roughness on the electrical parameters of industrial high efficiency NaOH-NaOCl textured multicrystalline silicon solar cell

    SciTech Connect

    Basu, P.K.; Pujahari, R.M.; Kaur, Harpreet; Singh, Devi; Varandani, D.; Mehta, B.R.

    2010-09-15

    Sodium hydroxide (NaOH) and sodium hypochlorite (NaOCl) solution (1:1 ratio by volume) based texturization process at 80-82 C is an easy, low cost and comparatively new and convenient option for fabrication of any multicrystalline silicon (mC-Si) solar cell. In the present study atomic force microscope is used to observe the intragrain surface in a miniscule area (3 {mu}m x 3 {mu}m) of NaOH-NaOCl textured surface by two and three dimensional analysis, roughness analysis and section analysis. The r.m.s value of the surface parameter of 7.0 nm ascertains the smoothness of the textured surface and further the surface reflectivity is minimized to 4-6% in the 500-1000 nm wavelength range by a proper silicon nitride anti-reflection coating. Comparing with the standard HF-HNO{sub 3}-CH{sub 3}COOH acid textured cell, the NaOH-NaOCl textured cell shows a comparatively lower value of series resistance of 7.17 m{omega}, higher value of shunt resistance of 18.4 {omega} to yield a fill factor of 0.766 leading to more than 15% cell efficiency in the industrial cell processing line. This AFM study yields different surface roughness parameters for the NaOH-NaOCl textured wafers which can be used as a reference standard for optimized texturing. (author)

  12. The Mon1–Ccz1 GEF activates the Rab7 GTPase Ypt7 via a longin-fold–Rab interface and association with PI3P-positive membranes

    PubMed Central

    Cabrera, Margarita; Nordmann, Mirjana; Perz, Angela; Schmedt, David; Gerondopoulos, Andreas; Barr, Francis; Piehler, Jacob; Engelbrecht-Vandré, Siegfried; Ungermann, Christian

    2014-01-01

    ABSTRACT To function in fusion and signaling, Rab GTPases need to be converted into their active GTP form. We previously identified the conserved Mon1–Ccz1 complex as the guanine nucleotide exchange factor (GEF) of the yeast Rab7 GTPase Ypt7. To address the possible GEF mechanism, we generated a homology model of the predicted longin domains of Mon1 and Ccz1 using the Rab-binding surface of the TRAPP complex as a template. On the basis of this, we identified mutations in both yeast Mon1 and Ccz1 that block Ypt7 activation, without affecting heterodimer formation and intracellular localization of Mon1 and Ccz1 at endosomes. Strikingly, the activity of the isolated Mon1–Ccz1 complex for Ypt7 is highly stimulated on membranes, and is promoted by the same anionic phospholipids such as phosphatidylinositol-3-phosphate (PI3P), which also support membrane association of the GEF complex. Our data imply that the GEF activity of the Mon1–Ccz1 complex towards Rab7/Ypt7 requires the interface formed by their longin domains and profits strongly from its association with the organelle surface. PMID:24413168

  13. Agriculture--Industry of the Future

    SciTech Connect

    2001-01-23

    This 8-page brochure describes the Office of Industrial Technologies' Agriculture Industry of the Future, a partnership between the Department of Energy and the agriculture industry established to increase industrial energy and cost efficiency.

  14. The Rac1-GEF Tiam1 couples the NMDA receptor to the activity-dependent development of dendritic arbors and spines.

    PubMed

    Tolias, Kimberley F; Bikoff, Jay B; Burette, Alain; Paradis, Suzanne; Harrar, Dana; Tavazoie, Sohail; Weinberg, Richard J; Greenberg, Michael E

    2005-02-17

    NMDA-type glutamate receptors play a critical role in the activity-dependent development and structural remodeling of dendritic arbors and spines. However, the molecular mechanisms that link NMDA receptor activation to changes in dendritic morphology remain unclear. We report that the Rac1-GEF Tiam1 is present in dendrites and spines and is required for their development. Tiam1 interacts with the NMDA receptor and is phosphorylated in a calcium-dependent manner in response to NMDA receptor stimulation. Blockade of Tiam1 function with RNAi and dominant interfering mutants of Tiam1 suggests that Tiam1 mediates effects of the NMDA receptor on dendritic development by inducing Rac1-dependent actin remodeling and protein synthesis. Taken together, these findings define a molecular mechanism by which NMDA receptor signaling controls the growth and morphology of dendritic arbors and spines. PMID:15721239

  15. High Power Impulse Magnetron Sputtering (HIPIMS) of Ultra-Hard and Low-Friction Nanocomposite Coatings for Improved Energy Efficiency and Durability in Demanding Industrial Applications

    SciTech Connect

    2009-03-01

    This factsheet describes a study whose goal is to explore the effectiveness of a revolutionary technology in industrial scale deposition systems – HIPIMS – in achieving the highest possible levels of adhesion between superhard, nanocomposite coatings and their substrates as well as super tough and strong cohesion within the films so that they cannot delaminate or crack when used under the very harsh and cycling operating conditions of advanced manufacturing and other industrial operations.

  16. Efficient Use of Energy

    ERIC Educational Resources Information Center

    Physics Today, 1975

    1975-01-01

    Explains efficiency in terms of thermodynamics, and states specific ways in which energy efficiency can be increased in the following areas: automobiles, industrial processes, and indoor use in the home. (MLH)

  17. Optimize Deployment of Renewable Energy Technologies for Government Agencies, Industrial Facilities, and Military Installations: NREL Offers Proven Tools and Resources to Reduce Energy Use and Improve Efficiency (Brochure)

    SciTech Connect

    Not Available

    2010-01-01

    The National Renewable Energy Lab provides expertise, facilities, and technical assistance to campuses, facilities, and government agencies to apply renewable energy and energy efficiency technologies.

  18. Energy Efficient Removal of Volatile Organic Compounds (VOCs) and Organic Hazardous Air Pollutants (o-HAPs) from Industrial Waste Streams by Direct Electron Oxidation

    SciTech Connect

    Testoni, A. L.

    2011-10-19

    This research program investigated and quantified the capability of direct electron beam destruction of volatile organic compounds and organic hazardous air pollutants in model industrial waste streams and calculated the energy savings that would be realized by the widespread adoption of the technology over traditional pollution control methods. Specifically, this research determined the quantity of electron beam dose required to remove 19 of the most important non-halogenated air pollutants from waste streams and constructed a technical and economic model for the implementation of the technology in key industries including petroleum refining, organic & solvent chemical production, food & beverage production, and forest & paper products manufacturing. Energy savings of 75 - 90% and green house gas reductions of 66 - 95% were calculated for the target market segments.

  19. Mutant Huntingtin Alters Cell Fate in Response to Microtubule Depolymerization via the GEF-H1-RhoA-ERK Pathway*

    PubMed Central

    Varma, Hemant; Yamamoto, Ai; Sarantos, Melissa R.; Hughes, Robert E.; Stockwell, Brent R.

    2010-01-01

    Cellular responses to drug treatment show tremendous variations. Elucidating mechanisms underlying these variations is critical for predicting therapeutic responses and developing personalized therapeutics. Using a small molecule screening approach, we discovered how a disease causing allele leads to opposing cell fates upon pharmacological perturbation. Diverse microtubule-depolymerizing agents protected mutant huntingtin-expressing cells from cell death, while being toxic to cells lacking mutant huntingtin or those expressing wild-type huntingtin. Additional neuronal cell lines and primary neurons from Huntington disease mice also showed altered survival upon microtubule depolymerization. Transcription profiling revealed that microtubule depolymerization induced the autocrine growth factor connective tissue growth factor and activated ERK survival signaling. The genotype-selective rescue was dependent upon increased RhoA protein levels in mutant huntingtin-expressing cells, because inhibition of RhoA, its downstream effector, Rho-associated kinase (ROCK), or a microtubule-associated RhoA activator, guanine nucleotide exchange factor-H1 (GEF-H1), all attenuated the rescue. Conversely, RhoA overexpression in cells lacking mutant huntingtin conferred resistance to microtubule-depolymerizer toxicity. This study elucidates a novel pathway linking microtubule stability to cell survival and provides insight into how genetic context can dramatically alter cellular responses to pharmacological interventions. PMID:20858895

  20. The budding yeast amphiphysin complex is required for contractile actin ring (CAR) assembly and post-contraction GEF-independent accumulation of Rho1-GTP.

    PubMed

    Cundell, Michael John; Price, Clive

    2014-01-01

    The late events of the budding yeast cell division cycle, cytokinesis and cell separation, require the assembly of a contractile actomyosin ring (CAR), primary and secondary septum formation followed by enzymatic degradation of the primary septum. Here we present evidence that demonstrates a role for the budding yeast amphiphysin complex, a heterodimer comprising Rvs167 and Rvs161, in CAR assembly and cell separation. The iqg1-1 allele is synthetically lethal with both rvs167 and rvs161 null mutations. We show that both Iqg1 and the amphiphysin complex are required for CAR assembly in early anaphase but cells are able to complete assembly in late anaphase when these activities are, respectively, either compromised or absent. Amphiphysin dependent CAR assembly is dependent upon the Rvs167 SH3 domain, but this function is insufficient to explain the observed synthetic lethality. Dosage suppression of the iqg1-1 allele demonstrates that endocytosis is required for the default cell separation pathway in the absence of CAR contraction but is unlikely to be required to maintain viability. The amphiphysin complex is required for normal, post-mitotic, localization of Chs3 and the Rho1 GEF, Rom2, which are responsible for secondary septum deposition and the accumulation of GTP bound Rho1 at the bud neck. It is concluded that a failure of polarity establishment in the absence of CAR contraction and amphiphysin function leads to loss of viability as a result of the consequent cell separation defect. PMID:24874185

  1. Distinct roles of RalA and RalB in the progression of cytokinesis are supported by distinct RalGEFs.

    PubMed

    Cascone, Ilaria; Selimoglu, Rasim; Ozdemir, Cafer; Del Nery, Elaine; Yeaman, Charles; White, Michael; Camonis, Jacques

    2008-09-17

    The Ras family G-proteins RalA and RalB make critical non-overlapping contributions to the generation of a tumorigenic regulatory network, supporting bypass of the normal restraints on both cell proliferation and survival. The Sec6/8 complex, or exocyst, has emerged as a principal direct effector complex for Ral GTPases. Here, we show that RalA and RalB support mitotic progression through mobilization of the exocyst for two spatially and kinetically distinct steps of cytokinesis. RalA is required to tether the exocyst to the cytokinetic furrow in early cytokinesis. RalB is then required for recruitment of the exocyst to the midbody of this bridge to drive abscission and completion of cytokinesis. The collaborative action of RalA and RalB is specified by discrete subcellular compartmentalization and unique pairs of RalGEF proteins that provide inputs from both Ras-family protein-dependent and protein-independent regulatory cues. This suggests that Ral GTPases integrate diverse upstream signals to choreograph multiple roles for the exocyst in mitotic progression. PMID:18756269

  2. Expression of the Rho-GEF Pbl/ECT2 is regulated by the UBE3A E3 ubiquitin ligase

    PubMed Central

    Reiter, Lawrence T.; Seagroves, Tiffany N.; Bowers, Megan; Bier, Ethan

    2013-01-01

    We applied genetic tools available in Drosophila to identify candidate substrates of the UBE3A ubiquitin ligase, the gene responsible for Angelman syndrome (AS). Human UBE3A was expressed in Drosophila heads to identify proteins differentially regulated in UBE3A-expressing versus wild-type extracts. Using two-dimensional gel and MALDI-TOF analysis, we detected 20 proteins that were differentially regulated by over-expression of human UBE3A in Drosophila heads. One protein responsive to UBE3A was the Rho-GEF pebble (pbl). Here, we present three lines of evidence suggesting that UBE3A regulates Pbl. First, we show genetic evidence that UBE3A and the Drosophila de-ubiquitinase fat facets (faf) exert opposing effects on Pbl function. Secondly, we find that both Pbl and ECT2, the mammalian orthologue of Pbl called epithelial cell transforming sequence 2 oncogene, physically interact with their respective ubiquitin E3 ligases. Finally, we show that Ect2 expression is regulated by Ube3a in mouse neurons as the pattern of Ect2 expression is dramatically altered in the hippocampus and cerebellum of Ube3a null mice. These results suggest that an orthologous UBE3A post-translational regulatory pathway regulates neuronal outgrowth in the mammalian brain and that dysregulation of this pathway may result in neurological phenotypes including AS and possibly other autism spectrum disorders. PMID:16905559

  3. Industrial diamond

    USGS Publications Warehouse

    Olson, D.W.

    2001-01-01

    An overview of the industrial diamond industry is provided. More than 90 percent of the industrial diamond consumed in the U.S. and the rest of the world is manufactured diamond. Ireland, Japan, Russia, and the U.S. produce 75 percent of the global industrial diamond output. In 2000, the U.S. was the largest market for industrial diamond. Industrial diamond applications, prices for industrial diamonds, imports and exports of industrial diamonds, the National Defense Stockpile of industrial diamonds, and the outlook for the industrial diamond market are discussed.

  4. IMPROVING ENERGY EFFICIENCY AND REDUCING COSTS IN THE DRINKING WATER SUPPLY INDUSTRY: An ENERGY STAR Resource Guide for Energy and Plant Managers

    SciTech Connect

    Melody, Moya; Dunham Whitehead, Camilla; Brown, Richard

    2010-09-30

    As American drinking water agencies face higher production costs, demand, and energy prices, they seek opportunities to reduce costs without negatively affecting the quality of the water they deliver. This guide describes resources for cost-effectively improving the energy efficiency of U.S. public drinking water facilities. The guide (1) describes areas of opportunity for improving energy efficiency in drinking water facilities; (2) provides detailed descriptions of resources to consult for each area of opportunity; (3) offers supplementary suggestions and information for the area; and (4) presents illustrative case studies, including analysis of cost-effectiveness.

  5. National Emission Standards for Hazardous Air Pollutants for Major Sources: Industrial, Commercial, and Institutional Boilers; Guidance for Calculating Efficiency Credits Resulting from Implementation of Energy Conservation Measures

    SciTech Connect

    Cox, Daryl; Papar, Riyaz; Wright, Dr. Anthony

    2013-02-01

    The purpose of this document is to provide guidance for developing a consistent approach to documenting efficiency credits generated from energy conservation measures in the Implementation Plan for boilers covered by the Boiler MACT rule (i.e., subpart DDDDD of CFR part 63). This document divides Boiler System conservation opportunities into four functional areas: 1) the boiler itself, 2) the condensate recovery system, 3) the distribution system, and 4) the end uses of the steam. This document provides technical information for documenting emissions credits proposed in the Implementation Plan for functional areas 2) though 4). This document does not include efficiency improvements related to the Boiler tune-ups.

  6. Studies on thio-substituted polyurethane foam (T-PUF) as a new efficient separation medium for the removal of inorganic/organic mercury from industrial effluents and solid wastes.

    PubMed

    Anjaneyulu, Y; Marayya, R; Rao, T H

    1993-01-01

    Novel thio-substituted flexible polyurethane foam (T-PUF) was synthesised by addition polymerisation of mercaptan with the precursors of an open-cell polyurethane foam, which can be used as a highly selective sorbent for inorganic and organic mercury from complex matrices. The percentage extraction of inorganic mercury was studied at different flow-rates, over a wide pH range at different concentrations ranging from 1 ppm, to 100 ppm. The break-through capacity and total capacity of unmodified and thio-foams were determined for inorganic and organic mercurials. The absorption efficiency of thio-foam was far superior to other sorbent media, such as activated carbon, polymeric ion-exchange resins and reagent-loaded polyurethane foams. It was observed that even at the 1000 ppm level, divalent ions like Cu, Mg, Ca, Zn do not appreciably influence the per cent extraction of inorganic mercury at the 10 ppm level. These matrix levels are the most concentrated ones which are likely to occur, both in local sewage and effluent waters. Further, the efficiency of this foam was sufficiently high at 10-100 ppm levels of Hg, even from 5-10 litres of effluent volumes using 50 g of thio-foam packed into different columns in series. Thio-foams were found to possess excellent abilities to remove and recover mercury even at low levels from industrial effluents and brine mud of chlor-alkali industry after pre-acid extraction. This makes it a highly efficient sorbent for possible application in effluent treatment. Model schemes for the removal and recovery of mercury from industrial effluents and municipal sewage (100-1000 litre) by a dynamic method are proposed and the costs incurred in a full-scale application method are indicated to show that the use of thio-foam could be commercially attractive. PMID:15091891

  7. Turning industry visions into reality

    SciTech Connect

    1997-01-01

    This brochure outlines the activities of the Office of Industrial Technologies (OIT) in the Department of Energy. OIT activities are aimed at industry adoption of energy-efficient, pollution-reducing technologies and include research and development on advanced technologies, financing, technical assistance, information dissemination, education, and bringing together industry groups, universities, National Laboratories, states, and environmentalists. OIT`s core initiative is to facilitate partnerships within seven materials and process industries: aluminum, chemicals, forest products, glass, metalcasting, petroleum refining, and steel industries.

  8. Ministry of Energy and Mineral Resources: The Hashemite Kingdom of Jordan industrial-energy-efficiency program. A report of the Office of Energy. Final report

    SciTech Connect

    Not Available

    1991-01-01

    There is a significant potential for energy savings in the small and medium-size industrial segment in Jordan. In order to achieve these savings, the Government of Jordan must take a more aggressive and coordinated role in promoting energy conservation through awareness and training programs. At the same time, the local market for C/E firms to provide energy conservation services can and should be developed. USAID and/or other donor agencies should assist the Government of Jordan through the funding of a medium-term technical assistance program, to help establish an autonomous or semi-autonomous energy conservation agency.

  9. Industrial garnet

    USGS Publications Warehouse

    Olson, D.W.

    2001-01-01

    A general overview of the industrial garnet industry is provided. About 20 percent of global industrial garnet production takes place in the U.S. During 2000, an estimated 300 kt of industrial garnets were produced worldwide. The U.S. is the world's largest consumer of industrial garnet, consuming 56.9 kt in 2000.

  10. Industry Support

    NASA Technical Reports Server (NTRS)

    2003-01-01

    NASA Glenn Research Center (GRC) is responsible for the Advanced Communications for Air Traffic Management (AC/ATM) Project, a sub-element task of the Advanced Air Transportation Technologies (AATT) Project of the NASA Aviation System Capacity Program (ASC). The AC/ATM Project is developing new communications technologies and tools that will improve throughput in the U.S. Air Traffic Control System. The goal of the AC/ATM Project is to enable a communications infrastructure providing the capacity, efficiency, and flexibility necessary to realize benefits of the future mature Free-Flight environment. The capabilities and scope of communications technologies needed to accomplish this goal depend on characteristics of the future Free-Flight environment. There are many operational concepts being proposed for a future ATM system to enable user flexibility and efficiency. GRC s focus is on developing new technologies and techniques to support the digital communication of information involving airborne and ground-based users. However, the technologies and techniques must be integrated with the systems and services that industry and the Federal Aviation Administration (FAA) are developing. Thus, GRC needs to monitor and provide input to the various industry and FAA organizations and committees that are specifying new systems and services. Adoption of technologies by the FAA is partially dependent on acceptance of the technology by the aviation community. The commercial aviation community in particular would like to adopt technologies that can be used throughout the world. As a result, the adoption of common or at least compatible technologies by European countries is a key factor in getting commitments to those technologies by the US aviation community. GRC desires to keep informed of European activities that relate to aviation communication technologies, particularly those that are being supported by Eurocontrol.

  11. Improving boiler efficiency

    SciTech Connect

    Yost, L.

    1982-06-24

    Boilers and burners are designed to operate most efficiently at, or near, full load. This fact seems to indicate that on/off operation is more efficient; however, standby losses must be considered. This article examines various types of industrial boiler heat losses that reduce efficiency and discusses methods for improving operation.

  12. The Rationale for Industrial Technology/Industrial Arts in Texas.

    ERIC Educational Resources Information Center

    Texas Education Agency, Austin.

    The goal of industrial technology for industrial arts education in Texas is the education of society to enable its members to function efficiently in the world of advancing technology. Policymakers in Texas have chosen to organize the industrial technology curriculum around three technology clusters: visual communication, production, and energy…

  13. Development and testing of a high efficiency advanced coal combustor phase III industrial boiler retrofit. Quarterly technical progress report No. 9, 1 October 1993--31 December 1993

    SciTech Connect

    Jennings, P.; Borio, R.; McGowan, J.G.

    1994-03-01

    This report documents the technical aspects of this project during the ninth quarter of the program. During this quarter, the natural gas baseline testing at the Penn State demonstration boiler was completed, results were analyzed and are presented here. The burner operates in a stable manner over an 8/1 turndown, however due to baghouse temperature limitations (300{degrees}F for acid dewpoint), the burner is not operated for long periods of time below 75% load. Boiler efficiency averaged 83.1% at the 100 percent load rate while increasing to 83.7% at 75% load. NO{sub x} emissions ranged from a low of 0.17 Lbs/MBtu to a high of 0.24 Lbs/MBtu. After the baseline natural gas testing was completed, work continued on hardware optimization and testing with the goal of increasing carbon conversion efficiency on 100% coal firing from {approx}95% to 98%. Several coal handling and feeding problems were encountered during this quarter and no long term testing was conducted. While resolving these problems several shorter term (less than 6 hour) tests were conducted. These included, 100% coal firing tests, 100% natural gas firing tests, testing of air sparges on coal to simulate more primary air and a series of cofiring tests. For 100% coal firing, the carbon conversion efficiency (CCE) obtained this quarter did not exceed the 95-96% barrier previously reached. NO{sub x} emissions on coal only ranged from {approx} 0.42 to {approx} 0.78 Lbs/MBtu. The burner has not been optimized for low NO{sub x} yet, however, due to the short furnace residence time, meeting the goals of 98% CCE and <0.6 Lbs/MBtu NO{sub x} simultaneously will be difficult. Testing on 100% natural gas in the boiler after coal firing indicated no changes in efficiency due to firing in a `dirty` boiler. The co-firing tests showed that increased levels of natural gas firing proportionately decreased NO{sub x}, SO{sub 2}, and CO.

  14. Industrial diamond

    USGS Publications Warehouse

    Olson, D.W.

    2003-01-01

    Statistics on the production, consumption, cost, trade, and government stockpile of natural and synthetic industrial diamond are provided. The outlook for the industrial diamond market is also considered.

  15. The cytohesin guanosine exchange factors (GEFs) are required to promote HGF-mediated renal recovery after acute kidney injury (AKI) in mice

    PubMed Central

    Reviriego-Mendoza, Marta M; Santy, Lorraine C

    2015-01-01

    The lack of current treatment and preventable measures for acute kidney injury (AKI) in hospitalized patients results in an increased mortality rate of up to 80% and elevated health costs. Additionally, if not properly repaired, those who survive AKI may develop fibrosis and long-term kidney damage. The molecular aspects of kidney injury and repair are still uncertain. Hepatocyte growth factor (HGF) promotes recovery of the injured kidney by inducing survival and migration of tubular epithelial cells to repopulate bare tubule areas. HGF-stimulated kidney epithelial cell migration requires the activation of ADP-ribosylation factor 6 (Arf6) and Rac1 via the cytohesin family of Arf-guanine-nucleotide exchange factors (GEFs), in vitro. We used an ischemia and reperfusion injury (IRI) mouse model to analyze the effects of modulating this signaling pathway on kidney recovery. We treated IRI mice with either HGF, the cytohesin inhibitor SecinH3, or a combination of both. As previously reported, HGF treatment promoted rapid improvement of kidney function as evidenced by creatinine (Cre) and blood urea nitrogen (BUN) levels. In contrast, simultaneous treatment with SecinH3 and HGF blocks the ability of HGF to promote kidney recovery. Immunohistochemistry showed that HGF treatment promoted recovery of tubule structure, and had enhanced levels of active, GTP-bound Arf6 and GTP-Rac1. SecinH3 treatment, however, caused a dramatic decrease in GTP-Arf6 and GTP-Rac1 levels when compared to kidney sections from HGF-treated IRI mice. Additionally, SecinH3 counteracted the renal reparative effects of HGF. Our results support the conclusion that cytohesin function is required for HGF-stimulated renal IRI repair. PMID:26116550

  16. CRB3A Controls the Morphology and Cohesion of Cancer Cells through Ehm2/p114RhoGEF-Dependent Signaling

    PubMed Central

    Loie, Elise; Charrier, Lucie E.; Sollier, Kévin; Masson, Jean-Yves

    2015-01-01

    The transmembrane protein CRB3A controls epithelial cell polarization. Elucidating the molecular mechanisms of CRB3A function is essential as this protein prevents the epithelial-to-mesenchymal transition (EMT), which contributes to tumor progression. To investigate the functional impact of altered CRB3A expression in cancer cells, we expressed CRB3A in HeLa cells, which are devoid of endogenous CRB3A. While control HeLa cells display a patchy F-actin distribution, CRB3A-expressing cells form a circumferential actomyosin belt. This reorganization of the cytoskeleton is accompanied by a transition from an ameboid cell shape to an epithelial-cell-like morphology. In addition, CRB3A increases the cohesion of HeLa cells. To perform these functions, CRB3A recruits p114RhoGEF and its activator Ehm2 to the cell periphery using both functional motifs of its cytoplasmic tail and increases RhoA activation levels. ROCK1 and ROCK2 (ROCK1/2), which are critical effectors of RhoA, are also essential to modulate the cytoskeleton and cell shape downstream of CRB3A. Overall, our study highlights novel roles for CRB3A and deciphers the signaling pathway conferring to CRB3A the ability to fulfill these functions. Thereby, our data will facilitate further investigation of CRB3A functions and increase our understanding of the cellular defects associated with the loss of CRB3A expression in cancer cells. PMID:26217016

  17. CRB3A Controls the Morphology and Cohesion of Cancer Cells through Ehm2/p114RhoGEF-Dependent Signaling.

    PubMed

    Loie, Elise; Charrier, Lucie E; Sollier, Kévin; Masson, Jean-Yves; Laprise, Patrick

    2015-10-01

    The transmembrane protein CRB3A controls epithelial cell polarization. Elucidating the molecular mechanisms of CRB3A function is essential as this protein prevents the epithelial-to-mesenchymal transition (EMT), which contributes to tumor progression. To investigate the functional impact of altered CRB3A expression in cancer cells, we expressed CRB3A in HeLa cells, which are devoid of endogenous CRB3A. While control HeLa cells display a patchy F-actin distribution, CRB3A-expressing cells form a circumferential actomyosin belt. This reorganization of the cytoskeleton is accompanied by a transition from an ameboid cell shape to an epithelial-cell-like morphology. In addition, CRB3A increases the cohesion of HeLa cells. To perform these functions, CRB3A recruits p114RhoGEF and its activator Ehm2 to the cell periphery using both functional motifs of its cytoplasmic tail and increases RhoA activation levels. ROCK1 and ROCK2 (ROCK1/2), which are critical effectors of RhoA, are also essential to modulate the cytoskeleton and cell shape downstream of CRB3A. Overall, our study highlights novel roles for CRB3A and deciphers the signaling pathway conferring to CRB3A the ability to fulfill these functions. Thereby, our data will facilitate further investigation of CRB3A functions and increase our understanding of the cellular defects associated with the loss of CRB3A expression in cancer cells. PMID:26217016

  18. Industrial partnerships yield EPIC results

    SciTech Connect

    Moore, T.

    1996-08-01

    With a new era of competition approaching in the electricity supply industry, utilities are getting closer than ever to their industrial customers, in many cases making direct alliances as partners to help customers become more efficient, productive, and competitive. The EPRI Partnership for Industrial Competitiveness (EPIC) program aims to help industrial customers address critical priorities in environmental impact, efficiency, and productivity with the ultimate goals of long-term profitability and job retention. By offering in-plant consultant evaluations of systems and processes, EPIC helps industrial customers develop strategic insights into their operations and leverage technology and productivity solutions for competitive business advantage.

  19. Effects of steam pretreatment and co-production with ethanol on the energy efficiency and process economics of combined biogas, heat and electricity production from industrial hemp

    PubMed Central

    2013-01-01

    Background The study presented here has used the commercial flow sheeting program Aspen Plus™ to evaluate techno-economic aspects of large-scale hemp-based processes for producing transportation fuels. The co-production of biogas, district heat and power from chopped and steam-pretreated hemp, and the co-production of ethanol, biogas, heat and power from steam-pretreated hemp were analysed. The analyses include assessments of heat demand, energy efficiency and process economics in terms of annual cash flows and minimum biogas and ethanol selling prices (MBSP and MESP). Results Producing biogas, heat and power from chopped hemp has the highest overall energy efficiency, 84% of the theoretical maximum (based on lower heating values), providing that the maximum capacity of district heat is delivered. The combined production of ethanol, biogas, heat and power has the highest energy efficiency (49%) if district heat is not produced. Neither the inclusion of steam pretreatment nor co-production with ethanol has a large impact on the MBSP. Ethanol is more expensive to produce than biogas is, but this is compensated for by its higher market price. None of the scenarios examined are economically viable, since the MBSP (EUR 103–128 per MWh) is higher than the market price of biogas (EUR 67 per MWh). The largest contribution to the cost is the cost of feedstock. Decreasing the retention time in the biogas process for low solids streams by partly replacing continuous stirred tank reactors by high-rate bioreactors decreases the MBSP. Also, recycling part of the liquid from the effluent from anaerobic digestion decreases the MBSP. The production and prices of methane and ethanol influence the process economics more than the production and prices of electricity and district heat. Conclusions To reduce the production cost of ethanol and biogas from biomass, the use of feedstocks that are cheaper than hemp, give higher output of ethanol and biogas, or combined production with

  20. Performance of a full-scale biofilter with peat and ash as a medium for treating industrial landfill leachate: a 3-year study of pollutant removal efficiency.

    PubMed

    Kängsepp, Pille; Mathiasson, Lennart

    2009-03-01

    Shredder residues of end-of-life vehicles and white goods are a complex waste stream, which nowadays most often is disposed of at industrial landfills. This paper describes the most important findings concerning the complex composition of the landfill leachate and its on-site, year-round treatment under cold-climate conditions. A 3-year investigation has confirmed that concentrations of different types of pollutants, most of them at low initial concentrations, can be simultaneously reduced in vertical-flow biofilters consisting of a mixture of peat and carbon-containing ash. For metals such as Mn, Cu, Sn, Cd, Pb, Fe and Ni the average removal was 73, 72, 66, 60, 55, 55 and 37%, respectively. An average reduction of NH(4)-N (45%), N(tot) (25%), total organic carbon (30%), dissolved organic carbon (28%) and suspended solids (38%) was also obtained. A good reduction was achieved for phenols (between 75 and 95%), polychlorinated biphenyls (between 22 and 99%), and gas chromatography-mass spectrometry amenable pollutants, considered at initial concentration above 50 microg L( -1) (between 80 and 100%). The performance of the biofilter system was good in spite of large variations of inlet concentration during the considered period. PMID:19244414

  1. The charge of ergonomics--a model according to the influence of ergonomic workplace design for economical and efficient indicators of the automotive industry.

    PubMed

    Neubert, Nicole; Bruder, Ralph; Toledo, Begoña

    2012-01-01

    The importance of ergonomic workplace design has been rising incredibly. The knowledge of the interaction with a view to many indicators (e.g. operators' health, quality, productivity etc.) in the automotive assembly shop pushed into another thinking of ergonomics and an increasing awareness of economic possibilities relating to benefits and cost savings aligned with ergonomics. The paper discusses exemplary the various indicators and factors which could be influenced by ergonomic workplace design. These factors are linked each other and support the statement of ergonomic efficiency. Thus, the aim of this paper is to present a model which describes that investments in ergonomic work placement acts with preventive measurements, minimization of losses (refinishing operations, compensation money etc.) and extensive economies on the whole company. PMID:22317394

  2. αPIX RhoGEF supports positive selection by restraining migration and promoting arrest of thymocytes.

    PubMed

    Korthals, Mark; Schilling, Kerstin; Reichardt, Peter; Mamula, Dejan; Schlüter, Thomas; Steiner, Michael; Langnäse, Kristina; Thomas, Ulrich; Gundelfinger, Eckart; Premont, Richard T; Tedford, Kerry; Fischer, Klaus-Dieter

    2014-04-01

    Thymocytes mature in a series of stages by migrating through specific areas of the thymus and interacting with other cells to receive the necessary developmental signals; however, little is known about the molecular mechanisms governing this migration. We report that murine thymocytes with a knockout mutation in α-PAK (p21-activated kinase)-interacting exchange factor (PIX; Arhgef6), an activator of Rho GTPases, showed greatly increased motility and altered morphology in two-dimensional migration on ICAM-1. αPIX was also required for efficient positive selection, but not negative selection, of thymocytes. TCR signaling was normal in αPix(-) thymocytes, indicating that the effects of αPIX on positive selection are largely independent of TCR signaling. αPix(-) thymocytes also paused less during migration in the thymic cortex, interacted less with ICAM-1 coated beads, and could overcome TCR stop signals, consistent with defective scanning behavior. These results identify αPIX as a regulator of thymocyte migration and subsequent arrest that is linked to positive selection. PMID:24591366

  3. Development and testing of a high efficiency advanced coal combustor: Phase 3 -- Industrial boiler retrofit. Proof of concept testing summary (Task 3.0 Final topical report)

    SciTech Connect

    Patel, R.L.; Borio, R.; McGowan, J.G.

    1995-07-01

    Economics may one day dictate that it makes sense to replace oil or natural gas with coal in boilers that were originally designed to burn oil or gas. In recognition of this future possibility, Pittsburgh Energy Technical Center (PETC) has supported a program led by ABB Power Plant Laboratories in cooperation with the Energy and Fuels Research Center of Penn State University to develop the High Efficiency Advanced Coal Combustor (HEACC). The objective of the program is to demonstrate the technical and economic feasibility of retrofitting a gas/oil designed boiler to burn micronized coal. In support of the overall objective the following specific areas were targeted: a coal handling/preparation system that can meet the technical requirements for retrofitting microfine coal on a boiler designed for burning oil or natural gas; maintaining boiler thermal performance in accordance with specifications when burning oil or natural gas; maintaining NOx emissions at or below 0.6 lb NO{sub 2} per million Btu; achieving combustion efficiencies of 98% or higher; and calculating economic payback periods as a function of key variables. The work carried out under this program is broken into five major Tasks: review of current state-of-the-art coal firing system components; design and experimental testing of a prototype HEACC burner; installation and testing of a HEACC system in a retrofit application; economic evaluation of the HEACC concept for retrofit applications; and long term demonstration under user demand conditions. This report summarizes the work done under Task 3, the installation and testing of the HEACC burner in a 15,000 lb/hr package boiler located at Penn State. The period of testing was approximately 400 hours. Key findings are presented.

  4. Industrial Minerals

    ERIC Educational Resources Information Center

    Bradbury, James C.

    1978-01-01

    The past year is seen as not particularly good for industrial minerals and for industry in general. Environmental concerns continued to trouble the industry with unacceptable asbestos concentrations and chlorofluorocarbon effects on ozone. A halting U.S. economy also affected industrial progress. (MA)

  5. Industry Employment

    ERIC Educational Resources Information Center

    Occupational Outlook Quarterly, 2012

    2012-01-01

    This article illustrates projected employment change by industry and industry sector over 2010-20 decade. Workers are grouped into an industry according to the type of good produced or service provided by the establishment for which they work. Industry employment projections are shown in terms of numeric change (growth or decline in the total…

  6. Industrial diamond

    USGS Publications Warehouse

    Olson, D.W.

    2000-01-01

    Part of the 1999 Industrial Minerals Review. A review of the state of the global industrial diamond industry in 1999 is presented. World consumption of industrial diamond has increased annually in recent years, with an estimated 500 million carats valued between $650 million and $800 million consumed in 1999. In 1999, the U.S. was the world's largest market for industrial diamond and was also one of the world's main producers; the others were Ireland, Russia, and South Africa. Uses of industrial diamonds are discussed, and prices of natural and synthetic industrial diamond are reported.

  7. Industrial Waste Reduction Program

    SciTech Connect

    Not Available

    1991-10-24

    US industry generates over 12 billion tons of wastes each year. These wastes consist of undesirable by-products of industrial production that are discarded into our environment. Energy is an integral part of these wastes; it is found in the embodied energy of industrial feedstocks not optimally used, in the energy content of the wastes themselves, and in the energy needed to transport, treat, and dispose of wastes. Estimates of the potential energy savings from reducing industrial wastes range from three to four quadrillion Btu per year -- enough to meet the annual energy needs of 30 million American homes. This document presents a plan for the Industrial Waste Reduction Program, which has been designed to help achieve national goals for energy efficiency and waste minimization. The objective of the program is to improve the energy efficiency of industrial processes through cost-effective waste reduction. The initial program focus is on waste reduction opportunities in the production and use of chemicals, due to the significant amount of energy used in these activities and the large amounts of hazardous and toxic wastes they generate. The chemical industry will be the initial subject of a series of waste reduction opportunity assessments conducted as part of the program. Assessments of other industries and waste problems will follow.

  8. Industrial Assessment Center

    SciTech Connect

    J. Kelly Kissock; Becky Blust

    2007-04-17

    The University of Dayton (UD) performed energy assessments, trained students and supported USDOE objectives. In particular, the UD Industrial Assessment Center (IAC) performed 96 industrial energy assessment days for mid-sized manufacturers. The average identified and implemented savings on each assessment were $261,080 per year and $54,790 per year. The assessments served as direct training in industrial energy efficiency for 16 UD IAC students. The assessments also served as a mechanism for the UD IAC to understand manufacturing energy use and improve upon the science of manufacturing energy efficiency. Specific research results were published in 16 conference proceedings and journals, disseminated in 22 additional invited lectures, and shared with the industrial energy community through the UD IAC website.

  9. Simultaneous removal of As, Cd, Cr, Cu, Ni and Zn from stormwater using high-efficiency industrial sorbents: Effect of pH, contact time and humic acid.

    PubMed

    Genç-Fuhrman, Hülya; Mikkelsen, Peter S; Ledin, Anna

    2016-10-01

    The effect of contact time, solution pH, and the presence of humic acid (HA) on the combined removal of As, Cd, Cr, Cu, Ni and Zn is investigated in batch tests using alumina, granulated activated carbon (GAC), and bauxsol coated sand (BCS) as sorbents. It is found that the equilibrium time for Cd, Cu, Ni and Zn is about 4h, while no clear equilibrium is observed for As and Cr. It is also found that increasing the pH until pH~8 enhanced Cd, Cu, Ni and Zn removal, but increasing the pH above this point had no major effect. In the cases of As and Cr, higher pH values (i.e. >7) decreased their removal. The presence of both 20 and 100mg/L HA suppressed the heavy metal removal except for Cr, and the suppression was higher at the higher HA concentration. Geochemical simulations suggest that this is due to the formation of dissolved HA-metal complexes preventing effective metal sorption. In the case of Cr, the presence of HA increased the removal when using alumina or BCS, while hindering the removal when using GAC. The findings show that the pH-value of the stormwater to be treated must be in the range of 6-7 in order to achieve removal of the full spectrum of metals. The results also show that natural organic matter may severely influence the removal efficiency, such that, for most metals the removal was reduced to the half, while for Cr it was increased to the double for alumina and BCS. Consequently, a properly working filter set up may not work properly anymore when receiving high loads of natural organic acids during the pollen season in spring or during defoliation in autumn and early winter, and during mixing of runoff with snowmelt having a low pH. PMID:27213673

  10. Industrial bronchitis

    MedlinePlus

    ... this page: //medlineplus.gov/ency/article/000072.htm Industrial bronchitis To use the sharing features on this page, please enable JavaScript. Industrial bronchitis is swelling (inflammation) of the large airways ...

  11. Industrial diamond

    USGS Publications Warehouse

    Olson, D.W.

    2004-01-01

    Part of the 2003 industrial minerals review. Supply and demand data for industrial diamond are provided. Topics discussed are consumption, prices, imports and exports, government stockpiles, and the outlook for 2004.

  12. Industrial diamond

    USGS Publications Warehouse

    Olson, D.W.

    2012-01-01

    Estimated 2011 world production of natural and synthetic industrial diamond was about 4.45 billion carats. During 2011, natural industrial diamonds were produced in more than 20 countries, and synthetic industrial diamond was produced in at least 13 countries. About 98 percent of the combined natural and synthetic global output was produced in China, Ireland, Japan, Russia, South Africa and the United States. China is the world's leading producer of synthetic industrial diamond followed by Russia and the United States.

  13. Industrial diamond

    USGS Publications Warehouse

    Olson, D.W.

    2011-01-01

    Estimated world production of natural and synthetic industrial diamond was about 4.44 billion carats in 2010. Natural industrial diamond deposits have been found in more than 35 countries, and synthetic industrial diamond is produced in at least 15 countries.

  14. Industrial diamond

    USGS Publications Warehouse

    Olson, D.W.

    2006-01-01

    In 2005, estimated world production of natural and synthetic industrial diamond was 630 million carats. Natural industrial diamond deposits were found in more than 35 countries. Synthetic industrial diamond is produced in at least 15 countries. More than 81% of the combined natural and synthetic global output was produced in Ireland, Japan, Russia, South Africa and the United States.

  15. Industry Employment

    ERIC Educational Resources Information Center

    Occupational Outlook Quarterly, 2010

    2010-01-01

    This article illustrates projected employment change from an industry perspective over the 2008-2018 decade. Workers are grouped into an industry according to the type of good produced or service provided by the establishment in which they work. Industry employment projections are shown in terms of numeric change (growth or decline in the total…

  16. Industrial Microbiology.

    ERIC Educational Resources Information Center

    Demain, Arnold L.; Solomon, Nadine A.

    1981-01-01

    Presents an overview of the field of industrial microbiology, providing historical backgrounds of scientific discoveries in the field and descriptions of industrially important microorganisms. Applied research in industry is also detailed, with mention of gene amplification, DNA recombination, pharmaceutical approaches, and detoxification and…

  17. Role of Appraisals in Energy Efficiency Financing

    SciTech Connect

    Doyle, V.; Bhargava, A.

    2012-05-01

    This research identifies barriers and challenges and current industry status including several key appraisal industry developments for identifying and valuing energy efficiency, critical obstacles to documenting and assessing the potential added value from energy efficiency improvements, current opportunities to support and standardize reporting on energy efficiency and to ensure proper valuation, and next steps towards enabling energy efficiency financing market transformation.

  18. Working with Industry

    ERIC Educational Resources Information Center

    Ullman, Ellen

    2012-01-01

    As community colleges focus on becoming more efficient and preparing more students for success in a climate of reduced state and federal funding, many institutions are reaching out to neighbors in business and industry, forming partnerships and working together to achieve goals that require money and resources colleges are unlikely to raise on…

  19. INTERMOUNTAIN INDUSTRIAL ASSESSMENT CENTER

    SciTech Connect

    MELINDA KRAHENBUHL

    2010-05-28

    The U. S. Department of Energy’s Intermountain Industrial Assessment Center (IIAC) at the University of Utah has been providing eligible small- and medium-sized manufacturers with no-cost plant assessments since 2001, offering cost-effective recommendations for improvements in the areas of energy efficiency, pollution prevention, and productivity improvement.

  20. 7 CFR 1220.112 - Industry information.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Soybean Promotion and Research Order Definitions § 1220.112 Industry information. The... markets, new marketing strategies, or increased efficiency for the soybean industry, and activities...

  1. 7 CFR 1220.112 - Industry information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Soybean Promotion and Research Order Definitions § 1220.112 Industry information. The... markets, new marketing strategies, or increased efficiency for the soybean industry, and activities...

  2. 7 CFR 1220.112 - Industry information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Soybean Promotion and Research Order Definitions § 1220.112 Industry information. The... markets, new marketing strategies, or increased efficiency for the soybean industry, and activities...

  3. Supporting industries energy and environmental profile

    SciTech Connect

    None, None

    2005-09-21

    As part of its Industries of the Future strategy, the Industrial Technologies Program within the U.S. Department of Energy’s (DOE) Office of Energy Efficiency and Renewable Energy works with energy-intensive industries to improve efficiency, reduce waste, and increase productivity. These seven Industries of the Future (IOFs) – aluminum, chemicals, forest products, glass, metal casting, mining, and steel – rely on several other so-called “supporting industries” to supply materials and processes necessary to the products that the IOFs create. The supporting industries, in many cases, also provide great opportunities for realizing energy efficiency gains in IOF processes.

  4. PtdIns(3,4,5)P3-dependent Rac Exchanger 1 (PREX1) Rac-Guanine Nucleotide Exchange Factor (GEF) Activity Promotes Breast Cancer Cell Proliferation and Tumor Growth via Activation of Extracellular Signal-regulated Kinase 1/2 (ERK1/2) Signaling.

    PubMed

    Liu, Heng-Jia; Ooms, Lisa M; Srijakotre, Nuthasuda; Man, Joey; Vieusseux, Jessica; Waters, JoAnne E; Feng, Yue; Bailey, Charles G; Rasko, John E J; Price, John T; Mitchell, Christina A

    2016-08-12

    PtdIns(3,4,5)P3-dependent Rac exchanger 1 (PREX1) is a Rac-guanine nucleotide exchange factor (GEF) overexpressed in a significant proportion of human breast cancers that integrates signals from upstream ErbB2/3 and CXCR4 membrane surface receptors. However, the PREX1 domains that facilitate its oncogenic activity and downstream signaling are not completely understood. We identify that ERK1/2 MAPK acts downstream of PREX1 and contributes to PREX1-mediated anchorage-independent cell growth. PREX1 overexpression increased but its shRNA knockdown decreased ERK1/2 phosphorylation in response to EGF/IGF-1 stimulation, resulting in induction of the cell cycle regulators cyclin D1 and p21(WAF1/CIP1) PREX1-mediated ERK1/2 phosphorylation, anchorage-independent cell growth, and cell migration were suppressed by inhibition of MEK1/2/ERK1/2 signaling. PREX1 overexpression reduced staurosporine-induced apoptosis whereas its shRNA knockdown promoted apoptosis in response to staurosporine or the anti-estrogen drug tamoxifen. Expression of wild-type but not GEF-inactive PREX1 increased anchorage-independent cell growth. In addition, mouse xenograft studies revealed that expression of wild-type but not GEF-dead PREX1 resulted in the formation of larger tumors that displayed increased phosphorylation of ERK1/2 but not AKT. The impaired anchorage-independent cell growth, apoptosis, and ERK1/2 signaling observed in stable PREX1 knockdown cells was restored by expression of wild-type but not GEF-dead-PREX1. Therefore, PREX1-Rac-GEF activity is critical for PREX1-dependent anchorage-independent cell growth and xenograft tumor growth and may represent a possible therapeutic target for breast cancers that exhibit PREX1 overexpression. PMID:27358402

  5. Industrial Robots.

    ERIC Educational Resources Information Center

    Reed, Dean; Harden, Thomas K.

    Robots are mechanical devices that can be programmed to perform some task of manipulation or locomotion under automatic control. This paper discusses: (1) early developments of the robotics industry in the United States; (2) the present structure of the industry; (3) noneconomic factors related to the use of robots; (4) labor considerations…

  6. Industrial Minerals.

    ERIC Educational Resources Information Center

    Brady, Lawrence L.

    1983-01-01

    Discusses trends in and factors related to the production of industrial minerals during 1982, indicating that, as 1981 marked a downturn in production of industrial minerals, 1982 continued the trend with temporary and permanent cutbacks in mine and plant production. Includes highlights of several conferences/conference papers in this field.…

  7. Industrial garnet

    USGS Publications Warehouse

    Olson, D.W.

    2000-01-01

    The state of the global industrial garnet industry in 1999 is discussed. Industrial garnet mined in the U.S., which accounts for approximately one-third of the world's total, is usually a solid-solution of almandine and pyrope. The U.S. is the largest consumer of industrial garnet, using an estimated 47,800 st in 1999 as an abrasive and as a filtration medium in the petroleum industry, filtration plants, aircraft and motor vehicle manufacture, shipbuilding, wood furniture finishing operations, electronic component manufacture, ceramics manufacture, and glass production. Prices for crude concentrates ranged from approximately $50 to $110/st and refined garnet from $50 to $215/st in 1999, depending on type, source, quantity purchased, quality, and application.

  8. Improving Compressed Air System Performance: A Sourcebook for Industry

    SciTech Connect

    2003-11-01

    NREL will produce this sourcebook for DOE's Industrial Technologies Office as part of a series of documents on industrial energy equipment. The sourcebook is a reference for industrial compressed air system users, outlining opportunities to improve system efficiency.

  9. INDUSTRIAL ASSESSMENT CENTER PROGRAM

    SciTech Connect

    ASFAW BEYENE

    2008-09-29

    Since its establishment in 1990, San Diego State University’s Industrial Assessment Center (IAC) has served close to 400 small and medium-sized manufacturing plants in Southern California. SDSU/IAC’s efforts to transfer state-of-the-art technologies to industry have increased revenues, cultivated creativity, improved efficiencies, and benefited the environment. A substantial benefit from the program has been the ongoing training of engineering faculty and students. During this funding cycle, SDSU/IAC has trained 31 students, 7 of the graduate. A total of 92 assessments and 108 assessment days were completed, resulting in 638 assessment recommendations.

  10. Industrial ecology.

    PubMed

    Patel, C K

    1992-02-01

    Industrial ecology addresses issues that will impact future production, use, and disposal technologies; proper use of the concept should reduce significantly the resources devoted to potential remediation in the future. This cradle-to-reincarnation production philosophy includes industrial processes that are environmentally sound and products that are environmentally safe during use and economically recyclable after use without adverse impact on the environment or on the net cost to society. This will require an industry-university-government round table to set the strategy and agenda for progress. PMID:11607254

  11. Experience Industry

    ERIC Educational Resources Information Center

    Laslett, R. L.

    1978-01-01

    The author, a tertiary chemistry teacher, spent a period of six weeks in a research project with an industrial firm. Describes his experience, the benefits and insight he gained from such an exchange. (GA)

  12. INDUSTRIAL WASTEWATER

    EPA Science Inventory

    This document contains: federal legislation, NRDC consent degree, regulations, the research program, and the different treatments used for petrochemistry, pesticides, inorganic chemicals, batteries, metal finishing, iron and steel, electric power, textiles and leather industries.

  13. Fermentation industry

    SciTech Connect

    Irvine, R.L.

    1980-06-01

    This article reviews current literature on the fermentation industry. The reuse, recycling and recovery of by-products previously discarded as waste are mentioned, including a Swedish brewery that hopes to reduce discharge of pollutants and the production of single cell protein from a variety of fermentation wastes. The treatment of wastes to produce food substitutes and fertilizers is mentioned together with treatment methods used in distilleries, wineries and in the pharmaceutical industry. (87 References)

  14. Industrial partnerships yield EPIC results

    SciTech Connect

    Moore, T.

    1996-01-01

    With a new era of competition approaching in the electricity supply industry, utilities are getting closer than ever to their industrial customers, in many cases making direct alliances as partners to help customers become more efficient, productive, and competitive. The EPRI Partnership for Industrial Competitiveness-EPIC-program aims to help industrial customers address critical priorities in environmental impact, efficiency and productivity with the ultimate goals of long-term profitability and job retention. By offering in-plant consultant evaluations of systems and processes, EPIC helps industrial customers develop strategic insights into their operations and leverage technology and productivity solutions for competitive business advantage. This paper describes these strategies, areas of focus, investments and benefits.

  15. Industrial Combustion Technology Roadmap: A Technology Roadmap by and for the Industrial Combustion Community (1999)

    SciTech Connect

    none,

    1999-04-01

    Combustion system users and manufacturers joined forces in 1999 to develop the Industrial Combustion Technology Roadmap. The roadmap outlines R&D priorities for developing advanced, highly efficient combustion systems that U.S. industry will require in the future.

  16. US cement industry

    SciTech Connect

    Nisbet, M.A.

    1997-12-31

    This paper describes the cement and concrete industry, and provides data on energy use and carbon dioxide emissions. The potential impact of an energy tax on the industry is briefly assessed. Opportunities identified for reducing carbon dioxide emissions include improved energy efficiency, alternative fuels, and alternative materials. The key factor in determining CO{sub 2} emissions is the level of domestic production. The projected improvement in energy efficiency and the relatively slow growth in domestic shipments indicate that CO{sub 2} emissions in 2000 should be about 5% above the 1990 target. However, due to the cyclical nature of cement demand, emissions will probably be above target levels during peak demand and below target levels during demand troughs. 7 figs., 2 tabs.

  17. Industrial systems biology.

    PubMed

    Otero, José Manuel; Nielsen, Jens

    2010-02-15

    The chemical industry is currently undergoing a dramatic change driven by demand for developing more sustainable processes for the production of fuels, chemicals, and materials. In biotechnological processes different microorganisms can be exploited, and the large diversity of metabolic reactions represents a rich repository for the design of chemical conversion processes that lead to efficient production of desirable products. However, often microorganisms that produce a desirable product, either naturally or because they have been engineered through insertion of heterologous pathways, have low yields and productivities, and in order to establish an economically viable process it is necessary to improve the performance of the microorganism. Here metabolic engineering is the enabling technology. Through metabolic engineering the metabolic landscape of the microorganism is engineered such that there is an efficient conversion of the raw material, typically glucose, to the product of interest. This process may involve both insertion of new enzymes activities, deletion of existing enzyme activities, but often also deregulation of existing regulatory structures operating in the cell. In order to rapidly identify the optimal metabolic engineering strategy the industry is to an increasing extent looking into the use of tools from systems biology. This involves both x-ome technologies such as transcriptome, proteome, metabolome, and fluxome analysis, and advanced mathematical modeling tools such as genome-scale metabolic modeling. Here we look into the history of these different techniques and review how they find application in industrial biotechnology, which will lead to what we here define as industrial systems biology. PMID:19891008

  18. Industrial diamond

    USGS Publications Warehouse

    Olson, D.W.

    2013-01-01

    Estimated 2012 world production of natural and synthetic industrial diamond was about 4.45 billion carats. During 2012, natural industrial diamonds were produced in at least 20 countries, and synthetic industrial diamond was produced in at least 12 countries. About 99 percent of the combined natural and synthetic global output was produced in Belarus, China, Ireland, Japan, Russia, South Africa and the United States. During 2012, China was the world’s leading producer of synthetic industrial diamond followed by the United States and Russia. In 2012, the two U.S. synthetic producers, one in Pennsylvania and the other in Ohio, had an estimated output of 103 million carats, valued at about $70.6 million. This was an estimated 43.7 million carats of synthetic diamond bort, grit, and dust and powder with a value of $14.5 million combined with an estimated 59.7 million carats of synthetic diamond stone with a value of $56.1 million. Also in 2012, nine U.S. firms manufactured polycrystalline diamond (PCD) from synthetic diamond grit and powder. The United States government does not collect or maintain data for either domestic PCD producers or domestic chemical vapor deposition (CVD) diamond producers for quantity or value of annual production. Current trade and consumption quantity data are not available for PCD or for CVD diamond. For these reasons, PCD and CVD diamond are not included in the industrial diamond quantitative data reported here.

  19. Materials needs and opportunities in the pulp and paper industry

    SciTech Connect

    Angelini, P.

    1995-08-01

    The Department of Energy`s (DOE) Office of Industrial Technologies (OIT) supports research and development (R&D) in industry, the DOE national laboratories, and in universities to develop energy efficient, environmentally-acceptable industrial technologies. The Office of Industrial Technologies is working with seven energy-intensive industries to develop R&D roadmaps that will facilitate cooperative government-industry efforts to achieve energy-efficient, environmentally-acceptable, sustainable industries of the future. The forest products industry is one of the industries with which OIT is working to develop an R&D roadmap. The Advanced Industrial Materials (AIM) Program of the Office of Industrial Technologies sponsors long-term, directed research on materials that will enable industry to develop and utilize more energy-efficient, sustainable processes and technologies. The purpose of the study described in this report was to identify the material R&D needs and opportunities for the pulp and paper mill of the future.

  20. 76 FR 34192 - Commercial and Industrial Pumps

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-13

    ... Part 431 RIN 1904-AC54 Commercial and Industrial Pumps AGENCY: Office of Energy Efficiency and... certain commercial and industrial equipment, and requires the Department of Energy (DOE) to administer an... commercial and industrial pumps. Additional input and suggestions relevant to this equipment are also...

  1. Infinitesimal Firms and Increasing Cost Industries.

    ERIC Educational Resources Information Center

    Peck, Richard M.

    2001-01-01

    Presents a rigorous version of the model of an increasing-cost competitive industry. Explains that in this model firms are infinitesimal, which justifies price-taking behavior and a continuous industry supply curve. Shows that the industry supply curve slopes upward because of dispersion in the efficiency of firms. Emphasizes role of marginal…

  2. Office of Industrial Technologies research in progress

    SciTech Connect

    Not Available

    1993-05-01

    The US Department of Energy (DOE) Office of Industrial Technologies (OIT) conducts research and development activities which focus on improving energy efficiency and providing for fuel flexibility within US industry in the area of industrial conservation. The mission of OIT is to increase the utilization of existing energy-efficient equipment and to find and promote new, cost-effective ways for industrial facilities to improve their energy efficiency and minimize waste products. To ensure advancement of the technological leadership of the United States and to improve the competitiveness of American industrial products in world markets, OIT works closely with industrial partners, the staffs of the national laboratories, and universities to identify research and development needs and to solve technological challenges. This report contains summaries of the currently active projects supported by the Office of Industrial Technologies.

  3. Industrial diamond

    USGS Publications Warehouse

    Olson, D.W.

    2007-01-01

    World production of natural and synthetic industrial diamond was about 648 million carats in 2006, with 79 percent of the production coming from Ireland, Japan, Russia, South Africa, and the U.S. U.S. consumption was was an estimated 602 million carats, imports were over 391 million carats, and exports were about 83 million carats. About 87 percent of the industrial diamonds market uses synthetic diamonds, which are expected to become less expensive as technology improves and competition from low-cost producers increases.

  4. Industrial garnet

    USGS Publications Warehouse

    Olson, D.W.

    2006-01-01

    In 2005, US production of crude garnet concentrate for industrial use was 28.4 kt valued at $3.05 million. Refined garnet material sold or used was 30.4 kt valued at $10 million. For the year, the US was one of the world's leading consumers of industrial garnet. Domestic values for crude concentrates for different applications ranged from about $53 to $120/t. In the short term, excess production capacity, combined with suppliers that vary in quality, grain size and mineral type, will keep prices down.

  5. Industrial Orientation.

    ERIC Educational Resources Information Center

    Rasor, Leslie; Brooks, Valerie

    These eight modules for an industrial orientation class were developed by a project to design an interdisciplinary program of basic skills training for disadvantaged students in a Construction Technology Program (see Note). The Drafting module overviews drafting career opportunities, job markets, salaries, educational requirements, and basic…

  6. Industry briefs

    SciTech Connect

    1990-11-01

    Recent nuclear industry briefs are presented. These briefs include: President Bush signs Radiation Victim Compensation Act; Japan Nuclear Fuel Completes construction of Rokkasho Enrichment Plant; Japanese vapor laser achieves 5% enrichment; US to cooperate with Hungary and Czechoslovakia on nuclear power; Soviet Union may reopen nuclear station in Armenia; despite earthquake concerns; and Japan`s Kashiwazak; Kaviwa - 2 begins commercial operation.

  7. Fermentation Industry.

    ERIC Educational Resources Information Center

    Grady, C. P. L., Jr.; Grady, J. K.

    1978-01-01

    Presents a literature review of wastes from the fermentation industry, covering publications of 1976-77. This review focuses on: (1) alcoholic beverage production; (2) pharmaceuticals and biochemicals production; and (3) biomass production. A list of 62 references is also presented. (HM)

  8. Industrial Microorganisms.

    ERIC Educational Resources Information Center

    Phaff, Herman J.

    1981-01-01

    Describes industrially important yeasts, molds, bacteria, and actinomycetes. Discussed in detail are microbial products, such as primary metabolites, secondary metabolites, enzymes, and capsular polysaccharides. Traces the historical background of human cell culture, mentioning recombinant DNA research and hybridization of normal mammalian cells…

  9. Industrial garnet

    USGS Publications Warehouse

    Olson, D.W.

    2011-01-01

    Garnet has been used as a gemstone since the Bronze Age. However, garnet's angular fractures, relatively high hardness and specific gravity, chemical inertness and nontoxicity make it ideal for many industrial applications. It is also free of crystalline silica and can be recycled.

  10. Industrial garnet

    USGS Publications Warehouse

    Olson, D.W.

    2013-01-01

    Garnet has been used as a gemstone since the Bronze Age. However, garnet’s angular fractures, relatively high hardness and specific gravity, chemical inertness and nontoxicity make it ideal for many industrial applications. It is also free of crystalline silica and can be recycled.

  11. Industrial garnet

    USGS Publications Warehouse

    Olson, D.W.

    2012-01-01

    Garnet has been used as a gemstone since the Bronze Age. However, garnet's angular fractures, relatively high hardness and specific gravity, chemical inertness, and nontoxicity make it ideal for many industrial applications. It is also free of crystalline silica and can be recycled.

  12. Industrial garnet

    USGS Publications Warehouse

    Olson, D.W.

    2010-01-01

    In 2009, U.S. production of crude garnet concentrate for industrial use was estimated to be 56.5 kt (62,300 st), valued at about $8.85 million. This was a 10-percent decrease in quantity compared with 2008 production. Refined garnet material sold or used was 28 kt (31,000 st) valued at $7.96 million.

  13. Research Projects in Industrial Technology.

    SciTech Connect

    United States. Bonneville Power Administration. Industrial Technology Section.

    1990-06-01

    The purpose of this booklet is to briefly describe ongoing and completed projects being carried out by Bonneville Power Administration's (BPA) Industrial Technology Section. In the Pacific Northwest, the industrial sector is the largest of the four consuming sectors. It accounted for thirty-nine percent of the total firm demand in the region in 1987. It is not easy to asses the conservation potential in the industrial sector. Recognizing this, the Northwest Power Planning Council established an objective to gain information on the size, cost, and availability of the conservation resource in the industrial sector, as well as other sectors, in its 1986 Power Plan. Specifically, the Council recommended that BPA operate a research and development program in conjunction with industry to determine the potential costs and savings from efficiency improvements in industrial processes which apply to a wide array of industrial firms.'' The section, composed of multidisciplinary engineers, provides technical support to the Industrial Programs Branch by designing and carrying out research relating to energy conservation in the industrial sector. The projects contained in this booklet are arranged by sector --industrial, utility, and agricultural -- and, within each sector, chronologically from ongoing to completed, with those projects completed most recently falling first. For each project the following information is given: its objective approach, key findings, cost, and contact person. Completed projects also include the date of completion, a report title, and report number.

  14. How a utility markets technology to industry

    SciTech Connect

    Kaczor, E.M.

    1986-03-01

    In response to economic contractions in its service area, Niagara Mohawk Power Company has instituted a technology transfer program for its industrial customers. The underlying motivation has been to maintain its industrial customers, as they provide a more substantial base to the economy than do service sector jobs. The program consists mainly of specialized seminars aimed at increasing industry's competitiveness by improving production efficiency. In addition, technical information is directly delivered to specific target industries.

  15. Energy efficient data centers

    SciTech Connect

    Tschudi, William; Xu, Tengfang; Sartor, Dale; Koomey, Jon; Nordman, Bruce; Sezgen, Osman

    2004-03-30

    Data Center facilities, prevalent in many industries and institutions are essential to California's economy. Energy intensive data centers are crucial to California's industries, and many other institutions (such as universities) in the state, and they play an important role in the constantly evolving communications industry. To better understand the impact of the energy requirements and energy efficiency improvement potential in these facilities, the California Energy Commission's PIER Industrial Program initiated this project with two primary focus areas: First, to characterize current data center electricity use; and secondly, to develop a research ''roadmap'' defining and prioritizing possible future public interest research and deployment efforts that would improve energy efficiency. Although there are many opinions concerning the energy intensity of data centers and the aggregate effect on California's electrical power systems, there is very little publicly available information. Through this project, actual energy consumption at its end use was measured in a number of data centers. This benchmark data was documented in case study reports, along with site-specific energy efficiency recommendations. Additionally, other data center energy benchmarks were obtained through synergistic projects, prior PG&E studies, and industry contacts. In total, energy benchmarks for sixteen data centers were obtained. For this project, a broad definition of ''data center'' was adopted which included internet hosting, corporate, institutional, governmental, educational and other miscellaneous data centers. Typically these facilities require specialized infrastructure to provide high quality power and cooling for IT equipment. All of these data center types were considered in the development of an estimate of the total power consumption in California. Finally, a research ''roadmap'' was developed through extensive participation with data center professionals, examination of case

  16. Improving pumping system efficiency at coal plants

    SciTech Connect

    Livoti, W.C.; McCandless, S.; Poltorak, R.

    2009-03-15

    The industry must employ ultramodern technologies when building or upgrading power plant pumping systems thereby using fuels more efficiently. The article discusses the uses and efficiencies of positive displacement pumps, centrifugal pumps and multiple screw pumps. 1 ref., 4 figs.

  17. Industry perspectives

    NASA Astrophysics Data System (ADS)

    Fuqua, Don; Hannemann, Tim; Iacobellis, Sam F.; Young, A. Thomas

    This discussion addresses the question of whether or not the space industry will take up the slack in the aerospace industry that is created by budget cuts in defense. Armed forces' research has provided an invaluable base for national technological and engineering resources for aerospace companies. It is noted that the space program should maximize the country's advances in national, scientific, and technical capabilities. It is also noted that NASA has done well in a most complicated business, but that Congress has failed to make a firm commitment to NASA's plans outlined in Vision 21 (based on the Augustine report) as evidenced by the Congressional reactions to NASA's budget requests. International cooperation and competition are discussed as well as the need for a political concensus on the direction of the space program in the U.S.

  18. Industrial alliances

    SciTech Connect

    Adams, K.V.

    1993-09-13

    The United States is emerging from the Cold War era into an exciting, but challenging future. Improving the economic competitiveness of our Nation is essential both for improving the quality of life in the United States and maintaining a strong national security. The research and technical skills used to maintain a leading edge in defense and energy now should be used to help meet the challenge of maintaining, regaining, and establishing US leadership in industrial technologies. Companies recognize that success in the world marketplace depends on products that are at the leading edge of technology, with competitive cost, quality, and performance. Los Alamos National Laboratory and its Industrial Partnership Center (IPC) has the strategic goal to make a strong contribution to the nation`s economic competitiveness by leveraging the government`s investment at the Laboratory: personnel, infrastructure, and technological expertise.

  19. Industrial radiointroscopy

    NASA Astrophysics Data System (ADS)

    Kliuev, V. V.; Leonov, B. I.; Gusev, E. A.

    The operating principles and design of various types of radiointroscopes used in industry for fault inspection are described, together with the sources of penetrating radiation, radiooptic image converters, and image amplifiers. The theory of image formation by a radiointroscope and mathematical models of the image formation are discussed. Consideration is given to the design of radioscope television systems, and their optical characteristics, resolving power, signal/noise characteristics, contrast sensitivity, and the transmission band of the communication channel.

  20. Industrial garnet

    USGS Publications Warehouse

    Olson, D.W.

    2007-01-01

    World production of industrial garnet was about 326 kt in 2006, with the U.S. producing about 11 percent of this total. U.S. consumption, imports, and exports were estimated at 74.3 kt, 52.3 kt, and 13.2 kt, respectively. The most important exporters are Australia, China, and India. Although demand is expected to rise over the next 5 years, prices are expected to remain low in the short term.

  1. Industrial Section Convenor's Report

    NASA Astrophysics Data System (ADS)

    Barone, M.; Riboni, P.

    2002-11-01

    Over the years this conference has gained a solid reputation as an appropriate rostrum for illustrating new concepts in the relations between industry and the scientific world and for introducing new technologies to a large assistance of junior and more experienced scientists. In fact, from the very beginning the founders of this endeavour announced: "The conference is aimed for promoting contacts among scientists involved in particle and fundamental physics, among experimental physicists in other fields and representatives from industry." Facilities at the Conference are designed to fulfil the task: space and general facilities are offered to industry representatives to display their products. This year a more accessible and luminous space arrangement was made available to the exhibitors. At the same time two plenary sessions have been dedicated to selected speakers to illustrate new trends in Technology Transfer, analysis of environment affecting our community, examples of historical successes in the merging of science and industry. We have identified in "GRID" and in "E-Publishing" two major promising areas where our Community will play a prime role as "User" and it was of the general interest to have them illustrated by two personalities directly involved in their development. The flow of knowledge is of course more massive from "Industry" to "Science" than vice-versa, but "Science" to "Industry" move offers an intensive added value. The technology transfer concept with the "Patents" as fund raising tool proved less glorious than expected. Trademark, licensing agreement and " Patents" can assure intellectual properties. But patent is an issue to be used cautiously. Evidence exists that much more efficient transfer of "Science" knowledge to "Economy" is achieved by venture capital move and start-up companies. These two facets of the Technology Transfer business have been covered by Routti's and Bourgeois's lectures.There are two examples of Companies who moved

  2. Mining Industry Energy Bandwidth Study

    SciTech Connect

    none,

    2007-07-01

    The Industrial Technologies Program (ITP) relies on analytical studies to identify large energy reduction opportunities in energy-intensive industries and uses these results to guide its R&D portfolio. The energy bandwidth illustrates the total energy-saving opportunity that exists in the industry if the current processes are improved by implementing more energy-efficient practices and by using advanced technologies. This bandwidth analysis report was conducted to assist the ITP Mining R&D program in identifying energy-saving opportunities in coal, metals, and mineral mining. These opportunities were analyzed in key mining processes of blasting, dewatering, drilling, digging, ventilation, materials handling, crushing, grinding, and separations.

  3. Industrial Technologies Program Research Plan for Energy-Intensive Process Industries

    SciTech Connect

    Chapas, Richard B.; Colwell, Jeffery A.

    2007-10-01

    In this plan, the Industrial Technologies Program (ITP) identifies the objectives of its cross-cutting strategy for conducting research in collaboration with industry and U.S. Department of Energy national laboratories to develop technologies that improve the efficiencies of energy-intensive process industries.

  4. Industrial concerns

    NASA Astrophysics Data System (ADS)

    2008-04-01

    Physics in industry is worth reporting, but deciding what counts can be a challenge "If a man can make a better mousetrap than his neighbour...the world will make a beaten path to his door." That quotation, which was apparently inspired by a comment that the American essayist Ralph Waldo Emerson made during a lecture in 1871, has long been used to illustrate the power of invention and innovation. While the lowly mousetrap may hardly seem the pinnacle of technology in today's world of iPhones and Blu-ray DVDs, an effective tool to kill mice was certainly a desirable object in Emerson's day (p52).

  5. Space Coatings for Industry

    NASA Technical Reports Server (NTRS)

    1980-01-01

    Ball Aerospace developed entirely new space lubrication technologies. A new family of dry lubricants emerged from Apollo, specifically designed for long life in space, together with processes for applying them to spacecraft components in microscopically thin coatings. Lubricants worked successfully on seven Orbiting Solar Observatory flights over the span of a decade and attracted attention to other contractors which became Ball customers. The company has developed several hundred variations of the original OSO technology generally designed to improve the quality and useful life of a wide range of products or improve efficiency of the industrial processes by which such products are manufactured.

  6. USMARC expands feed-efficiency research program

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Feed efficiency is not a new topic to the beef industry. Historically, this topic has been revisited by the industry every 10 to 15 years providing some benefit to the producer. The lack of progress in understanding the genetics of feed efficiency stems from the difficulty in trying to accurately ...

  7. The Role of Appraisals in Energy Efficiency Financing

    SciTech Connect

    Doyle, Victoria

    2012-05-01

    This research identifies barriers and challenges and current industry status including several key appraisal industry developments for identifying and valuing energy efficiency. The report covers critical obstacles to documenting and assessing the potential added value from energy efficiency improvements, current opportunities to support and standardize reporting on energy efficiency and to ensure proper valuation, and next steps towards enabling energy efficiency financing market transformation.

  8. Industrial Pumps

    NASA Technical Reports Server (NTRS)

    1986-01-01

    A flow inducer is a device that increases the pump intake capacity of a Worthington Centrifugal pump. It lifts the suction pressure sufficiently for the rotating main impeller of the centrifugal pump to operate efficiently at higher fluid intake levels. The concept derives from 1960's NASA technology which was advanced by Worthington Pump Division. The pumps are used to recirculate wood molasses, a highly viscous substance.

  9. Systems Biology of Industrial Microorganisms

    NASA Astrophysics Data System (ADS)

    Papini, Marta; Salazar, Margarita; Nielsen, Jens

    The field of industrial biotechnology is expanding rapidly as the chemical industry is looking towards more sustainable production of chemicals that can be used as fuels or building blocks for production of solvents and materials. In connection with the development of sustainable bioprocesses, it is a major challenge to design and develop efficient cell factories that can ensure cost efficient conversion of the raw material into the chemical of interest. This is achieved through metabolic engineering, where the metabolism of the cell factory is engineered such that there is an efficient conversion of sugars, the typical raw materials in the fermentation industry, into the desired product. However, engineering of cellular metabolism is often challenging due to the complex regulation that has evolved in connection with adaptation of the different microorganisms to their ecological niches. In order to map these regulatory structures and further de-regulate them, as well as identify ingenious metabolic engineering strategies that full-fill mass balance constraints, tools from systems biology can be applied. This involves both high-throughput analysis tools like transcriptome, proteome and metabolome analysis, as well as the use of mathematical modeling to simulate the phenotypes resulting from the different metabolic engineering strategies. It is in fact expected that systems biology may substantially improve the process of cell factory development, and we therefore propose the term Industrial Systems Biology for how systems biology will enhance the development of industrial biotechnology for sustainable chemical production.

  10. Distance collaborations with industry

    SciTech Connect

    Peskin, A.; Swyler, K.

    1998-06-01

    The college industry relationship has been identified as a key policy issue in Engineering Education. Collaborations between academic institutions and the industrial sector have a long history and a bright future. For Engineering and Engineering Technology programs in particular, industry has played a crucial role in many areas including advisement, financial support, and practical training of both faculty and students. Among the most important and intimate interactions are collaborative projects and formal cooperative education arrangements. Most recently, such collaborations have taken on a new dimension, as advances in technology have made possible meaningful technical collaboration at a distance. There are several obvious technology areas that have contributed significantly to this trend. Foremost is the ubiquitous presence of the Internet. Perhaps almost as important are advances in computer based imaging. Because visual images offer a compelling user experience, it affords greater knowledge transfer efficiency than other modes of delivery. Furthermore, the quality of the image appears to have a strongly correlated effect on insight. A good visualization facility offers both a means for communication and a shared information space for the subjects, which are among the essential features of both peer collaboration and distance learning.

  11. Development and testing of a high efficiency advanced coal combustor phase III industrial boiler retrofit. Technical progress report No. 17, 18 and 19, September 30, 1991--December 31, 1996

    SciTech Connect

    Borio, R.W.; Patel, R.L.; Thornock, D.E.

    1996-07-29

    The objective of this project is to retrofit a burner, capable of firing microfine coal, to a standard gas/oil designed industrial boiler to assess the technical and economic viability of displacing premium fuels with microfine coal. This report documents the technical aspects of this project during the last three quarters [seventeenth (October `95 through December `95), eighteenth (January `96 through March `96), and nineteenth (April `96 through June `96)] of the program.

  12. Industrial electrification trend shows strength

    SciTech Connect

    Burwell, C.C.

    1983-05-01

    One outcome of the oil embargo and cartel has been that, on the average, the price ratio of electricity to oil and gas has been cut in half. Some observers of the electric-utility industry may consider this change unimportant as electricity is still four times as expensive as oil or gas based on dollars per delivered Btu. For numerous uses, however, a given amount of electricity can replace several times as much fossil-fuel energy when relative efficiencies are considered. Thus with decreasing cost ratio, electricity can be justified by certain processes where it was previously uneconomical. A study of industrial activity suggests that several large industries are poised for a substantial shift to electric processing. Among them are the steel, aluminum, copper, glass, cement, petroleum, and paper industries. The potential and incentive for further electrification, from a thermodynamic standpoint, are strongest in manufacturing sectors that require high processing temperatures. 2 figures.

  13. The Resurgence of America's Auto Industry

    SciTech Connect

    Zimmer, Stephen; Cischke, Sue

    2012-01-01

    A look at how strategic investments and partnerships between the Energy Department and automakers have helped the American auto industry become a leader in advanced and fuel-efficient vehicles — creating jobs and boosting profits in the process.

  14. The Resurgence of America's Auto Industry

    ScienceCinema

    Zimmer, Stephen; Cischke, Sue;

    2013-05-29

    A look at how strategic investments and partnerships between the Energy Department and automakers have helped the American auto industry become a leader in advanced and fuel-efficient vehicles ? creating jobs and boosting profits in the process.

  15. EVALUATION OF THREE INDUSTRIAL PARTICULATE SCRUBBERS

    EPA Science Inventory

    The report gives results of field measurements, carried out on three full scale industrial scrubbers to determine scrubber performance characteristics, including particle collection efficiency as a function of particle diameter. The three scrubbers were different gas-atomized spr...

  16. Ceramics for ATS industrial turbines

    SciTech Connect

    Wenglarz, R.; Ali, S.; Layne, A.

    1996-05-01

    US DOE and most US manufacturers of stationary gas turbines are participating in a major national effort to develop advanced turbine systems (ATS). The ATS program will achieve ultrahigh efficiencies, environmental superiority, and cost competitiveness compared with current combustion turbine systems. A major factor in the improved efficiencies of simple cycle ATS gas turbines will be higher operating efficiencies than curren engines. These temperatures strain the limits of metallic alloy and flow-path cooling technologies. Ceramics materials offer a potential alterative to cooled turbine alloys for ATS turbines due to higher melting points than metallics. This paper evaluates ceramics technology and plant economic issues for ATS industrial turbine systems. A program with the objective of demonstrating first-stage ceramic vanes in a commerical industrial turbine is also described.

  17. 7 CFR 1215.8 - Industry information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 10 2014-01-01 2014-01-01 false Industry information. 1215.8 Section 1215.8 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... of new markets, new marketing strategies, or increased efficiency for the popcorn industry,...

  18. 7 CFR 1215.8 - Industry information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 10 2013-01-01 2013-01-01 false Industry information. 1215.8 Section 1215.8 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... of new markets, new marketing strategies, or increased efficiency for the popcorn industry,...

  19. Taxonomy of Industrial Penicillia and Aspergilli

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Proper identification of industrial strains of Aspergillus and Penicillium is important for a number of reasons, including: patenting, prevention of poisoning, quality control, and efficient screening for new products. To facilitate in meeting the needs of the various industries, systematists have...

  20. 7 CFR 1215.8 - Industry information.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Industry information. 1215.8 Section 1215.8 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... of new markets, new marketing strategies, or increased efficiency for the popcorn industry,...

  1. 7 CFR 1215.8 - Industry information.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 10 2011-01-01 2011-01-01 false Industry information. 1215.8 Section 1215.8 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... of new markets, new marketing strategies, or increased efficiency for the popcorn industry,...

  2. 7 CFR 1220.112 - Industry information.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Industry information. 1220.112 Section 1220.112 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... markets, new marketing strategies, or increased efficiency for the soybean industry, and activities...

  3. 7 CFR 1220.112 - Industry information.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 10 2011-01-01 2011-01-01 false Industry information. 1220.112 Section 1220.112 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... markets, new marketing strategies, or increased efficiency for the soybean industry, and activities...

  4. 7 CFR 1219.15 - Industry information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE HASS AVOCADO PROMOTION, RESEARCH, AND INFORMATION Hass Avocado Promotion, Research, and Information Order Definitions § 1219.15 Industry... efficiency, and enhance the image of Hass avocados and the Hass avocado industry in the United States....

  5. 7 CFR 1219.15 - Industry information.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE HASS AVOCADO PROMOTION, RESEARCH, AND INFORMATION Hass Avocado Promotion, Research, and Information Order Definitions § 1219.15 Industry... efficiency, and enhance the image of Hass avocados and the Hass avocado industry in the United States....

  6. 7 CFR 1219.15 - Industry information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE HASS AVOCADO PROMOTION, RESEARCH, AND INFORMATION Hass Avocado Promotion, Research, and Information Order Definitions § 1219.15 Industry... efficiency, and enhance the image of Hass avocados and the Hass avocado industry in the United States....

  7. The Rise and Fall of Industrial Agriculture

    ERIC Educational Resources Information Center

    Geno, Larry M.

    1976-01-01

    This article analyzes the evolution of industrial agriculture in Canada. Population pressures and technology caused the development of industrial agriculture. Although total crop yields have increased, energy efficiency and nutritional quality have decreased. Also intensive agriculture has degraded the soil and lowered air and water qualities. (MR)

  8. Industrial Productivity

    NASA Technical Reports Server (NTRS)

    1977-01-01

    NASTRAN is an offshoot of the computer-design technique used in construction of airplanes and spacecraft. [n this technique engineers create a mathematical model of the aeronautical or space vehicle and "fly" it on the ground by means of computer simulation. The technique enables them to study performance and structural behavior of a number of different designs before settling on the final configuration and proceeding with construction. From this base of aerospace experience, NASA-Goddard developed the NASTRAN general purpose computer program, which offers an exceptionally wide range of analytic capability with regard to structures. NASTRAN has been applied to autos, trucks, railroad cars, ships, nuclear power reactors, steam turbines, bridges, and office buildings. NASA-Langley provides program maintenance services regarded as vital by many NASTRAN users. NASTRAN is essentially a predictive tool. It takes an electronic look at a computerire$.dedgn and reports how the structure will react under a great many different conditions. It can, for example, note areas where high stress levels will occur-potential failure points that need strengthening. Conversely, it can identify over-designed areas where weight and material might be saved safely. NASTRAN can tell how pipes stand up under strong fluid flow, how metals are affected by high temperatures, how a building will fare in an earthquake or how powerful winds will cause a bridge to oscillate. NASTRAN analysis is quick and inexpensive. It minimizes trial-and-error in the design process and makes possible better, safe, lighter structures affording large-scale savings in development time and materials. Some examples of the broad utility NASTRAN is finding among industrial firms are shown on these pages.

  9. Application of Core Theory to the Airline Industry

    NASA Technical Reports Server (NTRS)

    Raghavan, Sunder

    2003-01-01

    Competition in the airline industry has been fierce since the industry was deregulated in 1978. The proponents of deregulation believed that more competition would improve efficiency and reduce prices and bring overall benefits to the consumer. In this paper, a case is made based on core theory that under certain demand and cost conditions more competition can actually lead to harmful consequences for industries like the airline industry or cause an empty core problem. Practices like monopolies, cartels, price discrimination, which is considered inefficient allocation of resources in many other industries, can actually be beneficial in the case of the airline industry in bringing about an efficient equilibrium.

  10. Energy Efficiency Project Development

    SciTech Connect

    IUEP

    2004-03-01

    The International Utility Efficiency Partnerships, Inc. (IUEP) has been a leader among the industry groups that have supported voluntary initiatives to promote international energy efficiency projects and address global climate change. The IUEP maintains its leadership by both supporting international greenhouse gas (GHG) reduction projects under the auspices of the U.S. Department of Energy (DOE) and by partnering with U.S. and international organizations to develop and implement strategies and specific energy efficiency projects. The goals of the IUEP program are to (1) provide a way for U.S. industry to maintain a leadership role in international energy efficiency infrastructure projects; (2) identify international energy project development opportunities to continue its leadership in supporting voluntary market-based mechanisms to reduce GHG emissions; and (3) demonstrate private sector commitment to voluntary approaches to global climate issues. The IUEP is dedicated to identifying, promoting, managing, and assisting in the registration of international energy efficiency projects that result in demonstrated voluntary reductions of GHG emissions. This Final Technical Report summarizes the IUEP's work in identifying, promoting, managing, and assisting in development of these projects and IUEP's effort in creating international cooperative partnerships to support project development activities that develop and deploy technologies that (1) increase efficiency in the production, delivery and use of energy; (2) increase the use of cleaner, low-carbon fuels in processing products; and (3) capture/sequester carbon gases from energy systems. Through international cooperative efforts, the IUEP intends to strengthen partnerships for energy technology innovation and demonstration projects capable of providing cleaner energy in a cost-effective manner. As detailed in this report, the IUEP met program objectives and goals during the reporting period January 1, 2001 through

  11. Efficient Windows Collaborative

    SciTech Connect

    Nils Petermann

    2010-02-28

    The project goals covered both the residential and commercial windows markets and involved a range of audiences such as window manufacturers, builders, homeowners, design professionals, utilities, and public agencies. Essential goals included: (1) Creation of 'Master Toolkits' of information that integrate diverse tools, rating systems, and incentive programs, customized for key audiences such as window manufacturers, design professionals, and utility programs. (2) Delivery of education and outreach programs to multiple audiences through conference presentations, publication of articles for builders and other industry professionals, and targeted dissemination of efficient window curricula to professionals and students. (3) Design and implementation of mechanisms to encourage and track sales of more efficient products through the existing Window Products Database as an incentive for manufacturers to improve products and participate in programs such as NFRC and ENERGY STAR. (4) Development of utility incentive programs to promote more efficient residential and commercial windows. Partnership with regional and local entities on the development of programs and customized information to move the market toward the highest performing products. An overarching project goal was to ensure that different audiences adopt and use the developed information, design and promotion tools and thus increase the market penetration of energy efficient fenestration products. In particular, a crucial success criterion was to move gas and electric utilities to increase the promotion of energy efficient windows through demand side management programs as an important step toward increasing the market share of energy efficient windows.

  12. Financial Assistance--Industries of the Future

    SciTech Connect

    2001-01-24

    This 8-page brochure describes the Office of Industrial Technologies' financial assistance programs--National Industrial Competitiveness through Energy, Environment, and Economics (NICE3) and Inventions and Innovation (I and I). Cost-shared grants awarded by NICE3 provide up to $500,000 to industry-state partnerships for demonstrations of clean and energy-efficient technologies. I and I awards grants of up to $200,000 to inventors who are establishing technical performance or in the early stages of development or commercialization of energy-efficient technologies.

  13. Exploring the resilience of industrial ecosystems.

    PubMed

    Zhu, Junming; Ruth, Matthias

    2013-06-15

    Industrial ecosystems improve eco-efficiency at the system level through optimizing material and energy flows, which however raises a concern for system resilience because efficiency, as traditionally conceived, not necessarily promotes resilience. By drawing on the concept of resilience in ecological systems and in supply chains, resilience in industrial ecosystems is specified on the basis of a system's ability to maintain eco-efficient material and energy flows under disruptions. Using a network model that captures supply, asset, and organizational dependencies and propagation of disruptions among firms, the resilience, and particularly resistance as an important dimension of resilience, of two real industrial ecosystems and generalized specifications are examined. The results show that an industrial ecosystem is less resistant and less resilient with high inter-firm dependency, preferentially organized physical exchanges, and under disruptions targeted at highly connected firms. An industrial ecosystem with more firms and exchanges is less resistant, but has more eco-efficient flows and potentials, and therefore is less likely to lose its function of eco-efficiency. Taking these determinants for resilience into consideration improves the adaptability of an industrial ecosystem, which helps increase its resilience. PMID:23562949

  14. Industrial Assessment Center Program Impact Evaluation

    SciTech Connect

    Martin, M.A.

    2000-01-26

    This report presents the results of an evaluation of the U.S. Department of Energy's Industrial Assessment Center (IAC) Program. The purpose of this program is to conduct energy, waste, and productivity assessments for small to medium-sized industrial firms. Assessments are conducted by 30 university-based industrial assessment centers. The purpose of this project was to evaluate energy and cost savings attributable to the assessments, the trained alumni, and the Websites sponsored by this program. How IAC assessments, alumni, and Web-based information may influence industrial energy efficiency decision making was also studied. It is concluded that appreciable energy and cost savings may be attributed to the IAC Program and that the IAC Program has resulted in more active and improved energy-efficiency decision making by industrial firms.

  15. Computer Technology for Industry

    NASA Technical Reports Server (NTRS)

    1979-01-01

    In this age of the computer, more and more business firms are automating their operations for increased efficiency in a great variety of jobs, from simple accounting to managing inventories, from precise machining to analyzing complex structures. In the interest of national productivity, NASA is providing assistance both to longtime computer users and newcomers to automated operations. Through a special technology utilization service, NASA saves industry time and money by making available already developed computer programs which have secondary utility. A computer program is essentially a set of instructions which tells the computer how to produce desired information or effect by drawing upon its stored input. Developing a new program from scratch can be costly and time-consuming. Very often, however, a program developed for one purpose can readily be adapted to a totally different application. To help industry take advantage of existing computer technology, NASA operates the Computer Software Management and Information Center (COSMIC)(registered TradeMark),located at the University of Georgia. COSMIC maintains a large library of computer programs developed for NASA, the Department of Defense, the Department of Energy and other technology-generating agencies of the government. The Center gets a continual flow of software packages, screens them for adaptability to private sector usage, stores them and informs potential customers of their availability.

  16. Chemicals Industry Vision

    SciTech Connect

    none,

    1996-12-01

    Chemical industry leaders articulated a long-term vision for the industry, its markets, and its technology in the groundbreaking 1996 document Technology Vision 2020 - The U.S. Chemical Industry. (PDF 310 KB).

  17. Professors and Industry Meet

    ERIC Educational Resources Information Center

    Sheriff, Robert E.

    1974-01-01

    Describes backgrounds of geophysics graduates that are desired for employment by industry. Also listed are areas in which industry could help universities concerning the development of programs to meet the future manpower needs in industry. (BR)

  18. High Efficiency, Clean Combustion

    SciTech Connect

    Donald Stanton

    2010-03-31

    Energy use in trucks has been increasing at a faster rate than that of automobiles within the U.S. transportation sector. According to the Energy Information Administration (EIA) Annual Energy Outlook (AEO), a 23% increase in fuel consumption for the U.S. heavy duty truck segment is expected between 2009 to 2020. The heavy duty vehicle oil consumption is projected to grow between 2009 and 2050 while light duty vehicle (LDV) fuel consumption will eventually experience a decrease. By 2050, the oil consumption rate by LDVs is anticipated to decrease below 2009 levels due to CAFE standards and biofuel use. In contrast, the heavy duty oil consumption rate is anticipated to double. The increasing trend in oil consumption for heavy trucks is linked to the vitality, security, and growth of the U.S. economy. An essential part of a stable and vibrant U.S. economy is a productive U.S. trucking industry. Studies have shown that the U.S. gross domestic product (GDP) is strongly correlated to freight transport. Over 90% of all U.S. freight tonnage is transported by diesel power and over 75% is transported by trucks. Given the vital role that the trucking industry plays in the economy, improving the efficiency of the transportation of goods was a central focus of the Cummins High Efficient Clean Combustion (HECC) program. In a commercial vehicle, the diesel engine remains the largest source of fuel efficiency loss, but remains the greatest opportunity for fuel efficiency improvements. In addition to reducing oil consumption and the dependency on foreign oil, this project will mitigate the impact on the environment by meeting US EPA 2010 emissions regulations. Innovation is a key element in sustaining a U.S. trucking industry that is competitive in global markets. Unlike passenger vehicles, the trucking industry cannot simply downsize the vehicle and still transport the freight with improved efficiency. The truck manufacturing and supporting industries are faced with numerous

  19. Analytical Chemistry in Industry.

    ERIC Educational Resources Information Center

    Kaiser, Mary A.; Ullman, Alan H.

    1988-01-01

    Clarifies the roles of a practicing analytical chemist in industry: quality control, methods and technique development, troubleshooting, research, and chemical analysis. Lists criteria for success in industry. (ML)

  20. Chemical Industry Corrosion Management

    SciTech Connect

    2003-02-01

    Improved Corrosion Management Could Provide Significant Cost and Energy Savings for the Chemical Industry. In the chemical industry, corrosion is often responsible for significant shutdown and maintenance costs.