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1

Using GenBank.  

PubMed

GenBank(R) is a comprehensive database of publicly available DNA sequences for more than 205,000 named organisms and for more than 60,000 within the embryophyta, obtained through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Daily data exchange with the European Molecular Biology Laboratory (EMBL) in Europe and the DNA Data Bank of Japan ensures worldwide coverage. GenBank is accessible through the National Center for Biotechnology Information (NCBI) retrieval system, Entrez, which integrates data from the major DNA and protein sequence databases with taxonomy, genome, mapping, protein structure, and domain information and the biomedical journal literature through PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available through FTP. GenBank usage scenarios ranging from local analyses of the data available through FTP to online analyses supported by the NCBI Web-based tools are discussed. To access GenBank and its related retrieval and analysis services, go to the NCBI Homepage at http://www.ncbi.nlm.nih.gov. PMID:18287687

Wheeler, David

2007-01-01

2

GenBank  

PubMed Central

GenBank® (http://www.ncbi.nlm.nih.gov/genbank/) is a comprehensive database that contains publicly available nucleotide sequences for over 300 000 formally described species. These sequences are obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects, including whole-genome shotgun and environmental sampling projects. Most submissions are made using the web-based BankIt or standalone Sequin programs, and GenBank staff assign accession numbers upon data receipt. Daily data exchange with the European Nucleotide Archive and the DNA Data Bank of Japan ensures worldwide coverage. GenBank is accessible through the NCBI Entrez retrieval system, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and the biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. PMID:25414350

Benson, Dennis A.; Clark, Karen; Karsch-Mizrachi, Ilene; Lipman, David J.; Ostell, James; Sayers, Eric W.

2015-01-01

3

DefinitionCode Gen Ed Code Definitions  

E-print Network

Studies Interdisciplinary Catalog # Gen Ed Class Title Gen Ed Classes: Fall 2014 DESCR.A AL AFROAM 117 Scandinavian Mythology 1 3/25/2014 #12;Catalog # Gen Ed Class Title Gen Ed Classes: Fall 2014 DESCR.A AL: Ancient-Medieval Art AT ART-HIST 100H Survey: Ancient-Medieval Art AT ART-HIST 115 Introduction To Visual

Massachusetts at Amherst, University of

4

Summary of CPAS Gen II Parachute Analysis  

NASA Technical Reports Server (NTRS)

The Orion spacecraft is currently under development by NASA and Lockheed Martin. Like Apollo, Orion will use a series of parachutes to slow its descent and splashdown safely. The Orion parachute system, known as the CEV Parachute Assembly System (CPAS), is being designed by NASA, the Engineering and Science Contract Group (ESCG), and Airborne Systems. The first generation (Gen I) of CPAS testing consisted of thirteen tests and was executed in the 2007-2008 timeframe. The Gen I tests provided an initial understanding of the CPAS parachutes. Knowledge gained from Gen I testing was used to plan the second generation of testing (Gen II). Gen II consisted of six tests: three singleparachute tests, designated as Main Development Tests, and three Cluster Development Tests. Gen II required a more thorough investigation into parachute performance than Gen I. Higher fidelity instrumentation, enhanced analysis methods and tools, and advanced test techniques were developed. The results of the Gen II test series are being incorporated into the CPAS design. Further testing and refinement of the design and model of parachute performance will occur during the upcoming third generation of testing (Gen III). This paper will provide an overview of the developments in CPAS analysis following the end of Gen I, including descriptions of new tools and techniques as well as overviews of the Gen II tests.

Morris, Aaron L.; Bledsoe, Kristin J.; Fraire, Usbaldo, Jr.; Moore, James W.; Olson, Leah M.; Ray, Eric

2011-01-01

5

tostandoutfromthecrowd? NextGen Immunology  

E-print Network

for Excellence in Immunology Become a leader of NextGen Immunology! The immune system, in addition to its with our microbial symbiotic partners (the microbiota) and aging, to brain function and cancer. As such to Immunology research Microbiome and host interactions in health and disease Immune cell development (thymus

Shapiro, Ehud

6

FutureGen Project Report  

SciTech Connect

This report summarizes the comprehensive siting, permitting, engineering, design, and costing activities completed by the FutureGen Industrial Alliance, the Department of Energy, and associated supporting subcontractors to develop a first of a kind near zero emissions integrated gasification combined cycle power plant and carbon capture and storage project (IGCC-CCS). With the goal to design, build, and reliably operate the first IGCC-CCS facility, FutureGen would have been the lowest emitting pulverized coal power plant in the world, while providing a timely and relevant basis for coal combustion power plants deploying carbon capture in the future. The content of this report summarizes key findings and results of applicable project evaluations; modeling, design, and engineering assessments; cost estimate reports; and schedule and risk mitigation from initiation of the FutureGen project through final flow sheet analyses including capital and operating reports completed under DOE award DE-FE0000587. This project report necessarily builds upon previously completed siting, design, and development work executed under DOE award DE-FC26- 06NT4207 which included the siting process; environmental permitting, compliance, and mitigation under the National Environmental Policy Act; and development of conceptual and design basis documentation for the FutureGen plant. For completeness, the report includes as attachments the siting and design basis documents, as well as the source documentation for the following: • Site evaluation and selection process and environmental characterization • Underground Injection Control (UIC) Permit Application including well design and subsurface modeling • FutureGen IGCC-CCS Design Basis Document • Process evaluations and technology selection via Illinois Clean Coal Review Board Technical Report • Process flow diagrams and heat/material balance for slurry-fed gasifier configuration • Process flow diagrams and heat/material balance for dry-fed gasifier configuration • Full capital cost report and cost category analysis (CAPEX) • Full operating cost report and assumptions (OPEX) Comparative technology evaluations, value engineering exercises, and initial air permitting activities are also provided; the report concludes with schedule, risk, and cost mitigation activities as well as lessons learned such that the products of this report can be used to support future investments in utility scale gasification and carbon capture and sequestration. Collectively, the FutureGen project enabled the comprehensive site specific evaluation and determination of the economic viability of IGCC-CCS. The project report is bound at that determination when DOE formally proposed the FutureGen 2.0 project which focuses on repowering a pulverized coal power plant with oxy-combustion technology including CCS.

Cabe, Jim; Elliott, Mike

2010-09-30

7

Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend.  

PubMed

The results of a phylogenetic and chemotaxonomic analysis of the genus Micrococcus indicated that it is significantly heterogeneous. Except for Micrococcus lylae, no species groups phylogenetically with the type species of the genus, Micrococcus luteus. The other members of the genus form three separate phylogenetic lines which on the basis of chemotaxonomic properties can be assigned to four genera. These genera are the genus Kocuria gen. nov. for Micrococcus roseus, Micrococcus varians, and Micrococcus kristinae, described as Kocuria rosea comb. nov., Kocuria varians comb. nov., and Kocuria kristinae comb. nov., respectively; the genus Nesterenkonia gen. nov. for Micrococcus halobius, described as Nesterenkonia halobia comb. nov.; the genus Nesterenkonia gen. nov. for Micrococcus halobius, described as Nesterenkonia halobia comb. nov.; the genus Dermacoccus gen. nov. for Micrococcus nishinomiyaensis, described as Dermacoccus nishinomiyaensis comb. nov.; and the genus Kytocossus gen. nov. for Micrococcus sedentarius, described as Kytococcus sedentarius comb. nov. M. luteus and M. lylae, which are closely related phylogenetically but differ in some chemotaxonomic properties, are the only species that remain in the genus Micrococcus Cohn 1872. An emended description of the genus Micrococcus is given [corrected]. PMID:7547287

Stackebrandt, E; Koch, C; Gvozdiak, O; Schumann, P

1995-10-01

8

AutoGen Version 5.0  

NASA Technical Reports Server (NTRS)

Version 5.0 of the AutoGen software has been released. Previous versions, variously denoted Autogen and autogen, were reported in two articles: Automated Sequence Generation Process and Software (NPO-30746), Software Tech Briefs (Special Supplement to NASA Tech Briefs), September 2007, page 30, and Autogen Version 2.0 (NPO- 41501), NASA Tech Briefs, Vol. 31, No. 10 (October 2007), page 58. To recapitulate: AutoGen (now signifying automatic sequence generation ) automates the generation of sequences of commands in a standard format for uplink to spacecraft. AutoGen requires fewer workers than are needed for older manual sequence-generation processes, and greatly reduces sequence-generation times. The sequences are embodied in spacecraft activity sequence files (SASFs). AutoGen automates generation of SASFs by use of another previously reported program called APGEN. AutoGen encodes knowledge of different mission phases and of how the resultant commands must differ among the phases. AutoGen also provides means for customizing sequences through use of configuration files. The approach followed in developing AutoGen has involved encoding the behaviors of a system into a model and encoding algorithms for context-sensitive customizations of the modeled behaviors. This version of AutoGen addressed the MRO (Mars Reconnaissance Orbiter) primary science phase (PSP) mission phase. On previous Mars missions this phase has more commonly been referred to as mapping phase. This version addressed the unique aspects of sequencing orbital operations and specifically the mission specific adaptation of orbital operations for MRO. This version also includes capabilities for MRO s role in Mars relay support for UHF relay communications with the MER rovers and the Phoenix lander.

Gladden, Roy E.; Khanampornpan, Teerapat; Fisher, Forest W.

2010-01-01

9

CoreGen and Chipscope Jim Duckworth Using CoreGen and ChipScope  

E-print Network

Generator System provides a catalog of ready-made functions, ranging in complexity from simple arithmeticCoreGen and Chipscope Jim Duckworth 1 Using CoreGen and ChipScope From Xilinx ... The Xilinx CORE operators like adders, accumulators, and multipliers, to system-level building blocks such as filters

Huang, Xinming

10

Debroyerella gen. nov. and Ulladulla gen. nov., two new lysianassoid genera (Crustacea, Amphipoda, Lysianassoidea).  

PubMed

Two new genera and a new species of lysianassoid amphipods are described. Debroyerella gen. nov. is described for three Antarctic species previously assigned to the genus Cheirimedon. Ulladulla gen. nov. is described to accommodate the new species U. selje, from Australian waters. Diagnostic descriptions are given for the genera and all species are described in full. PMID:25781243

Lowry, J K; Kilgallen, N M

2015-01-01

11

Unleashing Gen Y: Marketing Mars to Millennials  

NASA Technical Reports Server (NTRS)

Space advocates need to engage Generation Y (born 1977-1999).This outreach is necessary to recruit the next generation of scientists and engineers to explore Mars. Space advocates in the non-profit, private, and government sectors need to use a combination of technical communication, marketing, and politics, to develop messages that resonate with Gen Y. Until now, space messages have been generated by and for college-educated white males; Gen Y is much more diverse, including as much as one third minorities. Young women, too, need to be reached. My research has shown that messages emphasizing technology, fun, humor, and opportunity are the best means of reaching the Gen Y audience of 60 million (US population is 300 million). The important things space advocates must avoid are talking down to this generation, making false promises, or expecting them to "wait their turn" before they can participate. This is the MTV generation! We need to find ways of engaging Gen Y now to build a future where human beings can live and work on the planet Mars. In addition to the messages themselves, advocates need to keep up with Gen Y' s social networking and use of iPods, cell phones, and the Internet. NASA and space advocacy groups can use these tools for "viral marketing," where young people share targeted space-related information via cell phones or the Internet because they like it. Overall, Gen Y is a socially dynamic and media-savvy group; advocates' space messages need to be sincere, creative, and placed in locations where Gen Y lives. Mars messages must be memorable!

Leahy, Bart D.; Hidalgo, Loretta; Kloberdanz, Cassie

2007-01-01

12

24/04/2013 Next gen Sequencing technologies  

E-print Network

;1985 Human genome project proposed Start of the project in 1987 24/04/2013Next gen Sequencing technologies 7 "next gen" patents filed 2003 Human genome project finished 2005 first next gen sequencer available The illumina sequencer family: Genome AnalyzerIIx, HiSeq 2500, HiSeq 2000, HiSeq 1500, HiSeq 1000, Hi

Twente, Universiteit

13

The Integrated Airport: Building a Successful NextGen Testbed  

ScienceCinema

This presentation will describe a unique public-private partnership - the Integrated Airport - that was created to engage in research and testing related to NextGen Technology deployment.  NextGen refers to the program that will be initiated to modernize the US National Airspace.  As with any major, multi-decade initiative, such as NextGen, integration of work efforts by multiple partners in the modernization is critical for success.  This talk will focus on the development of the consortium, how the consortium plans for NextGen initiatives, the series of technology demonstrations we have produced and plans for the future of NextGen testing and implementation. 

Christina Frederick-Recascino

2010-01-08

14

Gen IV Materials Handbook Implementation Plan  

SciTech Connect

A Gen IV Materials Handbook is being developed to provide an authoritative single source of highly qualified structural materials information and materials properties data for use in design and analyses of all Generation IV Reactor Systems. The Handbook will be responsive to the needs expressed by all of the principal government, national laboratory, and private company stakeholders of Gen IV Reactor Systems. The Gen IV Materials Handbook Implementation Plan provided here addresses the purpose, rationale, attributes, and benefits of the Handbook and will detail its content, format, quality assurance, applicability, and access. Structural materials, both metallic and ceramic, for all Gen IV reactor types currently supported by the Department of Energy (DOE) will be included in the Gen IV Materials Handbook. However, initial emphasis will be on materials for the Very High Temperature Reactor (VHTR). Descriptive information (e.g., chemical composition and applicable technical specifications and codes) will be provided for each material along with an extensive presentation of mechanical and physical property data including consideration of temperature, irradiation, environment, etc. effects on properties. Access to the Gen IV Materials Handbook will be internet-based with appropriate levels of control. Information and data in the Handbook will be configured to allow search by material classes, specific materials, specific information or property class, specific property, data parameters, and individual data points identified with materials parameters, test conditions, and data source. Details on all of these as well as proposed applicability and consideration of data quality classes are provided in the Implementation Plan. Website development for the Handbook is divided into six phases including (1) detailed product analysis and specification, (2) simulation and design, (3) implementation and testing, (4) product release, (5) project/product evaluation, and (6) product maintenance and enhancement. Contracting of development of the Handbook website is discussed in terms of host server options, cost, technology, developer background and cooperative nature, and company stability. One of the first and most important activities in website development will be the generation of a detailed Handbook product requirements document including case diagrams and functional requirements tables. The Implementation Plan provides a detailed overview of the organizational structure of the Handbook and details of Handbook preparation, publication, and distribution. Finally, the Implementation Plan defines Quality Assurance requirements for the Handbook.

Rittenhouse, P.; Ren, W.

2005-03-29

15

GenJam: A Genetic Algorithm for Generating Jazz Solos  

Microsoft Academic Search

This paper describes GenJam, a genetic algorithm-based model of a novice jazz musician learning to improvise. GenJam maintains hierarchically related populations of melodic ideas that are mapped to specific notes through scales suggested by the chord progression being played. As GenJam plays its solos over the accompaniment of a standard rhythm section, a human mentor gives real-time feedback, which is

John A. Biles

1994-01-01

16

Complications Following BellaGen™ Injection  

PubMed Central

BellaGen™ is an injectable acellular dermal matrix granule derived from donated human skin tissue that was recently developed for soft tissue augmentation. Its use has been sporadically reported in penile girth enhancement procedures. Many cases of complications have been reported after injecting acellular dermal matrices like AlloDerm or SureDerm™ but few reports on complications associated with BellaGen™ injection. We report here on penile skin inflammation and necrosis following augmentation phalloplasty with BellaGen™, which developed 3 days after the injection and persisted for more than 2 weeks. The patient had a 15 year history of type 2 diabetes mellitus, and he was treated with oral antibiotics and wet dressings with KMNO4 solution to combine preservation of the remaining penile soft tissue with debridement of avascular tissue. The lesion improved with this treatment, but the patient experienced pain for about 2 weeks. All patients should be informed of the potential complications with the use of injectable acellular dermal matrices before treatment. Patient selection for augmentation is also important to have the most desirable results. PMID:22346264

Park, Mi Kyung; Kim, Hyun Kyu; Park, Kui Young; Li, Kapsok; Hong, Chang Kwun

2011-01-01

17

NCBI Handout Series | MedGen | Last Update March 9, 2014 Contact: info@ncbi.nlm.nih.gov MedGen: A Medical Genetics Portal  

E-print Network

Gen: A Medical Genetics Portal A public web portal providing access to medical genetics information availableVar, GeneReviews, and Medical Genetics Summaries on NCBI's bookshelf. Data from MedGen can be searchedGen: Portal to Medical Genetics Information Contact: info@ncbi.nlm.nih.gov NCBI Handout Series | MedGen | Last

Levin, Judith G.

18

77 FR 2342 - Fifth Meeting: RTCA, Next Gen Advisory Committee  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF TRANSPORTATION Federal Aviation Administration Fifth Meeting: RTCA, Next Gen Advisory Committee AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation (DOT)....

2012-01-17

19

New records of the Cryphonectriaceae from southern Africa including Latruncellus aurorae gen. sp. nov.  

E-print Network

New records of the Cryphonectriaceae from southern Africa including Latruncellus aurorae gen. sp, Latruncellus aurorae gen. sp. nov., is described from Galpinia transvaalica (Lythraceae, Myrtales) in Swazi

20

Effect of Gen2 Protocol Parameters on RFID Tag Performance  

E-print Network

Effect of Gen2 Protocol Parameters on RFID Tag Performance Pavel V. Nikitin and K. V. S. Rao.rao@intermec.com Abstract-- In this paper, we analyze the effect of Gen2 protocol parameters on RFID tag performance (tag. INTRODUCTION Today, the dependence of UHF RFID tag performance on various tag parameters (chip sensitivity, tag

Hochberg, Michael

21

Towards an International Culture: Gen Y Students and SNS?  

ERIC Educational Resources Information Center

This article reports the findings of a small-scale investigation into the Internet user behaviour of generation Y (Gen Y) students, with particular reference to social networking sites. The study adds to the literature on cross-cultural Internet user behaviour with specific reference to Gen Y and social networking. It compares how a cohort of…

Lichy, Jessica

2012-01-01

22

ProGen: GPHMM for prokaryotic genomes Sharad Akshar Punuganti  

E-print Network

ProGen: GPHMM for prokaryotic genomes Sharad Akshar Punuganti May 10, 2011 Abstract Pro and implemented to train the model and find the genes respectively. ProGen models prokaryotic genome (hence of GPHMMs in the domain of prokaryotic genomes as the genomic structure of prokaryotes is relatively simple

Liblit, Ben

23

TidGen Power System Commercialization Project  

SciTech Connect

ORPC Maine, LLC, a wholly-owned subsidiary of Ocean Renewable Power Company, LLC (collectively ORPC), submits this Final Technical Report for the TidGen® Power System Commercialization Project (Project), partially funded by the U.S. Department of Energy (DE-EE0003647). The Project was built and operated in compliance with the Federal Energy Regulatory Commission (FERC) pilot project license (P-12711) and other permits and approvals needed for the Project. This report documents the methodologies, activities and results of the various phases of the Project, including design, engineering, procurement, assembly, installation, operation, licensing, environmental monitoring, retrieval, maintenance and repair. The Project represents a significant achievement for the renewable energy portfolio of the U.S. in general, and for the U.S. marine hydrokinetic (MHK) industry in particular. The stated Project goal was to advance, demonstrate and accelerate deployment and commercialization of ORPC’s tidal-current based hydrokinetic power generation system, including the energy extraction and conversion technology, associated power electronics, and interconnection equipment capable of reliably delivering electricity to the domestic power grid. ORPC achieved this goal by designing, building and operating the TidGen® Power System in 2012 and becoming the first federally licensed hydrokinetic tidal energy project to deliver electricity to a power grid under a power purchase agreement in North America. Located in Cobscook Bay between Eastport and Lubec, Maine, the TidGen® Power System was connected to the Bangor Hydro Electric utility grid at an on-shore station in North Lubec on September 13, 2012. ORPC obtained a FERC pilot project license for the Project on February 12, 2012 and the first Maine Department of Environmental Protection General Permit issued for a tidal energy project on January 31, 2012. In addition, ORPC entered into a 20-year agreement with Bangor Hydro Electric Company on January 1, 2013 for up to 5 megawatts at a price of $215/MWh, escalating at 2.0% per year.

Sauer, Christopher R. [President & CEO] [President & CEO; McEntee, Jarlath [VP Engineering & CTO] [VP Engineering & CTO

2013-12-30

24

Gen IV Materials Handbook Functionalities and Operation  

SciTech Connect

This document is prepared for navigation and operation of the Gen IV Materials Handbook, with architecture description and new user access initiation instructions. Development rationale and history of the Handbook is summarized. The major development aspects, architecture, and design principles of the Handbook are briefly introduced to provide an overview of its past evolution and future prospects. Detailed instructions are given with examples for navigating the constructed Handbook components and using the main functionalities. Procedures are provided in a step-by-step fashion for Data Upload Managers to upload reports and data files, as well as for new users to initiate Handbook access.

Ren, Weiju [ORNL

2009-12-01

25

Back to the FutureGen?  

SciTech Connect

After years of political wrangling, Democrats may green-light the experimental clean coal power plants. The article relates how the project came to be curtailed, how Senator Dick Durbin managed to protect $134 million in funding for FutureGen in Mattoon, and how once Obama was in office a $2 billion line item to fund a 'near zero emissions power plant(s)' was placed in the Senate version of the Stimulus Bill. The final version of the legislation cut the funding to $1 billion for 'fossil energy research and development'. In December 2008 the FutureGen Alliance and the City of Mattoon spent $6.5 billion to purchase the plants eventual 440 acre site. A report by the Government Accountability Office (GAO) said that Bush's inaction may have set back clean coal technology in the US by as much as a decade. If additional funding comes through construction of the plant could start in 2010. 1 fig., 1 photo.

Buchsbaum, L.

2009-04-15

26

Head-worn displays for NextGen  

NASA Astrophysics Data System (ADS)

The operating concepts emerging under the Next Generation air transportation system (NextGen) require new technology and procedures - not only on the ground-side - but also on the flight deck. Flight deck display and decision support technologies are specifically targeted to overcome aircraft safety barriers that might otherwise constrain the full realization of NextGen. One such technology is the very lightweight, unobtrusive head-worn display (HWD). HWDs with an integrated head-tracking system are being researched as they offer significant potential benefit under emerging NextGen operational concepts. Two areas of benefit for NextGen are defined. First, the HWD may be designed to be equivalent to the Head-Up Display (HUD) using Virtual HUD concepts. As such, these operational credits may be provided to significantly more aircraft for which HUD installation is neither practical nor possible. Second, the HWD provides unique display capabilities, such as an unlimited field-of-regard. These capabilities may be integral to emerging NextGen operational concepts, eliminating safety issues which might otherwise constrain the full realization of NextGen. The paper details recent research results, current HWD technology limitations, and future technology development needed to realize HWDs as a enabling technology for NextGen.

Bailey, Randall E.; Shelton, Kevin J.; Arthur, J. J., III

2011-06-01

27

RxGen General Optical Model Prescription Generator  

NASA Technical Reports Server (NTRS)

RxGen is a prescription generator for JPL's in-house optical modeling software package called MACOS (Modeling and Analysis for Controlled Optical Systems), which is an expert optical analysis software package focusing on modeling optics on dynamic structures, deformable optics, and controlled optics. The objectives of RxGen are to simplify and automate MACOS prescription generations, reducing errors associated with creating such optical prescriptions, and improving user efficiency without requiring MACOS proficiency. RxGen uses MATLAB (a high-level language and interactive environment developed by MathWorks) as the development and deployment platform, but RxGen can easily be ported to another optical modeling/analysis platform. Running RxGen within the modeling environment has the huge benefit that variations in optical models can be made an integral part of the modeling state. For instance, optical prescription parameters determined as external functional dependencies, optical variations by controlling the in-/exclusion of optical components like sub-systems, and/or controlling the state of all components. Combining the mentioned capabilities and flexibilities with RxGen's optical abstraction layer completely eliminates the hindering aspects for requiring proficiency in writing/editing MACOS prescriptions, allowing users to focus on the modeling aspects of optical systems, i.e., increasing productivity and efficiency. RxGen provides significant enhancements to MACOS and delivers a framework for fast prototyping as well as for developing very complex controlled optical systems.

Sigrist, Norbert

2012-01-01

28

Head-Worn Displays for NextGen  

NASA Technical Reports Server (NTRS)

The operating concepts emerging under the Next Generation air transportation system (NextGen) require new technology and procedures - not only on the ground-side - but also on the flight deck. Flight deck display and decision support technologies are specifically targeted to overcome aircraft safety barriers that might otherwise constrain the full realization of NextGen. One such technology is the very lightweight, unobtrusive head-worn display (HWD). HWDs with an integrated head-tracking system are being researched as they offer significant potential benefit under emerging NextGen operational concepts. Two areas of benefit for NextGen are defined. First, the HWD may be designed to be equivalent to the Head-Up Display (HUD) using Virtual HUD concepts. As such, these operational credits may be provided to significantly more aircraft for which HUD installation is neither practical nor possible. Second, the HWD provides unique display capabilities, such as an unlimited field-of-regard. These capabilities may be integral to emerging NextGen operational concepts, eliminating safety issues which might otherwise constrain the full realization of NextGen. The paper details recent research results, current HWD technology limitations, and future technology development needed to realize HWDs as a enabling technology for NextGen.

Bailey, Randall E.; Shelton, Kevin J.; Arthur, J. J.

2011-01-01

29

NextGen Future Safety Assessment Game  

NASA Technical Reports Server (NTRS)

The successful implementation of the next generation infrastructure systems requires solid understanding of their technical, social, political and economic aspects along with their interactions. The lack of historical data that relate to the long-term planning of complex systems introduces unique challenges for decision makers and involved stakeholders which in turn result in unsustainable systems. Also, the need to understand the infrastructure at the societal level and capture the interaction between multiple stakeholders becomes important. This paper proposes a methodology in order to develop a holistic approach aiming to provide an alternative subject-matter expert (SME) elicitation and data collection method for future sociotechnical systems. The methodology is adapted to Next Generation Air Transportation System (NextGen) decision making environment in order to demonstrate the benefits of this holistic approach.

Ancel, Ersin; Gheorghe, Adian; Jones, Sharon Monica

2010-01-01

30

NSF StatGen 2009 Bayesian Interval Mapping  

E-print Network

? ­ identify units of natural selection ­ additive effects may be most important (Wright/Fisher debate) NSF Churchill) NSF StatGen: Yandell © 2009 3 Marker Trait problems of single QTL approach · wrong model: biased

Yandell, Brian S.

31

College of Agriculture, Food and Environment GEN General Agriculture  

E-print Network

College of Agriculture, Food and Environment GEN General Agriculture KEY: # = new course * = course IN AGRICULTURE. (3) Anintroductorycourserequiringcriticalanalysisofthemajorsocial. Prereq: Students enrolled in the College of Agriculture; freshmen only in fall semesters and transfers

MacAdam, Keith

32

EPCGen2 Pseudorandom Number Generators: Analysis of J3Gen  

PubMed Central

This paper analyzes the cryptographic security of J3Gen, a promising pseudo random number generator for low-cost passive Radio Frequency Identification (RFID) tags. Although J3Gen has been shown to fulfill the randomness criteria set by the EPCglobal Gen2 standard and is intended for security applications, we describe here two cryptanalytic attacks that question its security claims: (i) a probabilistic attack based on solving linear equation systems; and (ii) a deterministic attack based on the decimation of the output sequence. Numerical results, supported by simulations, show that for the specific recommended values of the configurable parameters, a low number of intercepted output bits are enough to break J3Gen. We then make some recommendations that address these issues. PMID:24721767

Peinado, Alberto; Munilla, Jorge; Fúster-Sabater, Amparo

2014-01-01

33

GEN_ENG 20102011 Course No. Title Fall Winter Spring  

E-print Network

206120 Honors Engineering Analysis I 11 MF #12;GEN_ENG 20102011 Course No. Title Fall Winter Spring 206220 Honors Engineering Analysis II 12 MF 206320 Honors Engineering Analysis III 11 MF 206420* Honors Engineering Analysis 1 MF

MacIver, Malcolm A.

34

1. GENERAL VIEW. Statues: Maj. Gen George Meade by Daniel ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. GENERAL VIEW. Statues: Maj. Gen George Meade by Daniel Chester French, south side; Maj. Gen. John Reynolds by Charles Grafly, north side. Equestrian Statues: Maj. Gen George B. McClellan by Edward C. Potter, south side; Maj. Gen Winfield S. Hancock by J.Q.A. Ward, north side. The statue at the base of northern inner pedestal is Richard Smith, a type founder and donor of the Memorial. The niches are filled with eight colossal busts including Union generals, admirals, Pennsylvania governor, Memorial's architects (John T. and James H. Windrim), and executor of Smith's will. The frieze is carved with the names of eighty-four prominent Pennsylvania participants in the Civil War. - Smith Memorial Arch, Philadelphia, Philadelphia County, PA

35

EPCGen2 pseudorandom number generators: analysis of J3Gen.  

PubMed

This paper analyzes the cryptographic security of J3Gen, a promising pseudo random number generator for low-cost passive Radio Frequency Identification (RFID) tags. Although J3Gen has been shown to fulfill the randomness criteria set by the EPCglobal Gen2 standard and is intended for security applications, we describe here two cryptanalytic attacks that question its security claims: (i) a probabilistic attack based on solving linear equation systems; and (ii) a deterministic attack based on the decimation of the output sequence. Numerical results, supported by simulations, show that for the specific recommended values of the configurable parameters, a low number of intercepted output bits are enough to break J3Gen. We then make some recommendations that address these issues. PMID:24721767

Peinado, Alberto; Munilla, Jorge; Fúster-Sabater, Amparo

2014-01-01

36

GenMAPP and MAPPFinder for Systems Biology Education  

NSDL National Science Digital Library

The GenMAPP and MAPPFinder system provides a framework for learning about the properties of biochemical and gene regulatory pathways. Students can research a pathway in conjunction with a course topic and then draw it out with the GenMAPP graphics tools. Students can then color-code the pathway with publicly available microarray data, connecting them to cutting-edge research. The pathways the students draw can be submitted to the public pathway repository at www.GenMAPP.org, their work making a contribution to practicing scientists. In summary, the systems level approach provided by GenMAPP moves seamlessly from class projects to independent research and will allow students to make a contribution to the scientific community.

Kam Dahlquist (Loyola Marymount University; )

2004-06-12

37

GenTegra DNA Tubes is another proprietary technology for stor-ing purified DNA in a `bone-dry', water-free environment. This new  

E-print Network

(STR) analysis, human leukocyte antigen (HLA) typing and genotyping assays from all com- mon analysis of products that includes GenPlatesTM, GenSolveTM, GenTegraTMTubes and GenConnectTM to store and manage

Cai, Long

38

SEQ-GEN: A comprehensive multimission sequencing system  

NASA Technical Reports Server (NTRS)

SEQ-GEN is a user-interactive computer program used to plan and generate a sequence of commands for spacecraft. Desired activities are specified by the user of SEQ-GEN; SEQ-GEN in turn expands these activities, deriving the spacecraft commands necessary to accomplish the desired activities. SEQ-GEN models the effects on the spacecraft of the commands, predicting the state as a function of time, flagging any conflicts and rule violations. These states, conflicts, and violations are viewable both graphically and textually at the user's request. SEQ-GEN also displays the entire sequence graphically, showing each requested activity as a bar on its graphical timeline. SEQ-GEN immediately revalidates the sequence, updating its models and calculations along with its displays based on these changes. Because it has the ability to recalculate spacecraft states immediately, the user is able to perform 'what-if' sessions easily. SEQ-GEN, a multimission tool, is adaptable to any flight project. A flight project writes its adaptation files containing project unique information including in its simplest form, only spacecraft commands. For more involved projects the adaptation files may also contain flight and mission rules, description of the spacecraft and ground models, and the definition of activities. SEQ-GEN operates at whatever level of detail the adaptation files imply. Simple adaptations are straight forward to do. There is, however, no limit to the complexity of activity definitions or of spacecraft models: both may involve unlimited logical decision points. Commands and activities may involve any number of parameters of a wide variety of data types, including integer, float, time, boolean, and character strings. SEQ-GEN will be used by the Mars Pathfinder, Cassini, and VIM (Voyager Interstellar Mission) project in an effort to speed up adaptation time and to keep sequence generation costs down. SEQ-GEN is hosted on UNIX workstations. It uses MOTIF and X for windowing, and was designed and coded in an object-oriented style in the language C++.

Salcedo, Jose; Starbird, Thomas J.

1994-01-01

39

Parachloroidium gen. nov. (Trebouxiophyceae, Chlorophyta), a novel genus of coccoid green algae from subaerial corticolous biofilms  

E-print Network

Parachloroidium gen. nov. (Trebouxiophyceae, Chlorophyta), a novel genus of coccoid green algae. Parachloroidium gen. nov. (Trebouxiophyceae, Chlorophyta), a novel genus of coccoid green algae from subaerial the Parachloroidium strains from other similar green algae. However, ultrastructural characteristics and molecular

40

A Virtual Reality Framework to Optimize Design, Operation and Refueling of GEN-IV Reactors.  

SciTech Connect

many GEN-IV candidate designs are currently under investigation. Technical issues related to material, safety and economics are being addressed at research laboratories, industry and in academia. After safety, economic feasibility is likely to be the most important crterion in the success of GEN-IV design(s). Lessons learned from the designers and operators of GEN-II (and GEN-III) reactors must play a vital role in achieving both safety and economic feasibility goals.

Rizwan-uddin; Nick Karancevic; Stefano Markidis; Joel Dixon; Cheng Luo; Jared Reynolds

2008-04-23

41

GenI: Natural language generation in Haskell INRIA/LORIA/UHP  

E-print Network

GenI: Natural language generation in Haskell Eric Kow INRIA/LORIA/UHP eric.kow@loria.fr Abstract In this article we present GenI, a chart based surface realisation tool implemented in Haskell. GenI takes corresponds to the input semantics. The aim of the article is not so much to present GenI or to de- scribe how

Paris-Sud XI, Université de

42

HydroGen: Automatically Generating Self-Assembly Code for Hydron Units  

E-print Network

HydroGen: Automatically Generating Self-Assembly Code for Hydron Units George Konidaris, Tim Taylor, jhallamg@inf.ed.ac.uk Abstract. This paper introduces HydroGen, an object compiler system that produces, and are thus free of the constraints of gravity. This system, called HydroGen, is therefore able to assemble

Massachusetts at Amherst, University of

43

Training trust in automation within a NextGen environment  

NASA Astrophysics Data System (ADS)

The projected increase in air traffic density has led to the development of NextGen that is expected to revolutionize the way the NAS is currently functioning. For NextGen to be implemented successfully, it is important to understand the humanautomation relationship within a complex system. Controllers will have to rely on automated tools to successfully manage aircraft in their sector. Increasing human trust in automation can lead to an increase in automation acceptance and its proper use. However, we are not aware of any research studies that have attempted to directly train individuals to trust automation. We report on an attempt to train 8 novice air traffic controllers to trust the automated NextGen tools in a radar internship course. Although the results were not statistically significant, the trend in the data suggests that it may be possible to train trust in automation.

Higham, Tiana-Lynn M.

44

DOE/NNSA perspective safeguard by design: GEN III/III+ light water reactors and beyond  

SciTech Connect

An overview of key issues relevant to safeguards by design (SBD) for GEN III/IV nuclear reactors is provided. Lessons learned from construction of typical GEN III+ water reactors with respect to SBD are highlighted. Details of SBD for safeguards guidance development for GEN III/III+ light water reactors are developed and reported. This paper also identifies technical challenges to extend SBD including proliferation resistance methodologies to other GEN III/III+ reactors (except HWRs) and GEN IV reactors because of their immaturity in designs.

Pan, Paul Y [Los Alamos National Laboratory

2010-12-10

45

UCT Finance Policies & Procedures GEN004 Page 1 of 3  

E-print Network

university, educating for life and addressing the challenges facing our society." Policy & Title Effective date GEN004 ­ Cell phone policy 1 February 2005 Objective This policy has been introduced to: · clearly define who is entitled to a UCT cell phone and how the costs of purchasing the asset plus ancillary

Jarrett, Thomas H.

46

GenOVa: a computer program to generate orientational variants  

PubMed Central

A computer program called GenOVa, written in Python, calculates the orientational variants, the operators (special types of misorientations between variants) and the composition table associated with a groupoid structure. The variants can be represented by three-dimensional shapes or by pole figures. PMID:19461844

Cayron, Cyril

2007-01-01

47

An electronic flight bag for NextGen avionics  

NASA Astrophysics Data System (ADS)

The introduction of the Next Generation Air Transportation System (NextGen) initiative by the Federal Aviation Administration (FAA) will impose new requirements for cockpit avionics. A similar program is also taking place in Europe by the European Organisation for the Safety of Air Navigation (Eurocontrol) called the Single European Sky Air Traffic Management Research (SESAR) initiative. NextGen will require aircraft to utilize Automatic Dependent Surveillance-Broadcast (ADS-B) in/out technology, requiring substantial changes to existing cockpit display systems. There are two ways that aircraft operators can upgrade their aircraft in order to utilize ADS-B technology. The first is to replace existing primary flight displays with new displays that are ADS-B compatible. The second, less costly approach is to install an advanced Class 3 Electronic Flight Bag (EFB) system. The installation of Class 3 EFBs in the cockpit will allow aircraft operators to utilize ADS-B technology in a lesser amount of time with a decreased cost of implementation and will provide additional benefits to the operator. This paper describes a Class 3 EFB, the NexisTM Flight-Intelligence System, which has been designed to allow users a direct interface with NextGen avionics sensors while additionally providing the pilot with all the necessary information to meet NextGen requirements.

Zelazo, D. Eyton

2012-06-01

48

Growing threats posed by patho-gens--whether in intentional  

E-print Network

Analysis and Notification of Antigen Risks and Yields). · CANARY uses nature's bioidentifiers, B cellsGrowing threats posed by patho- gens--whether in intentional bioagent attacks or through uninten method that can provide a warning before the target population is infected. To meet this need, MIT

Reuter, Martin

49

, INRAAdaptation des Herbivores aux Theix 63122 Saint-Gens-Champanelle  

E-print Network

B. DUMONT , INRAAdaptation des Herbivores aux Milieux Theix 63122 Saint-Genès-Champanelle Déterminisme des choix alimentaires des herbivores au pâturage : principales théories Comment mieux gérer les zones agricoles en déprise ? Comment les herbivores peuvent-ils contribuer à entretenir ces espaces

Paris-Sud XI, Université de

50

Using GenRel for reliability assessment of mining equipment  

Microsoft Academic Search

Purpose – The purpose of this paper is to formulate, develop and test a reliability assessment model (GenRel) based on genetic algorithms. Design\\/methodology\\/approach – Using genetic algorithm based modelling technique, a computer model was developed to predict mine equipment failures from historical data. Two different approaches in application of this technique are demonstrated. Findings – A case study representing a

Nick Vayenas; Greg Yuriy

2007-01-01

51

A New Parent Generation: Meet Mr. and Mrs. Gen X  

ERIC Educational Resources Information Center

Slowly but surely, Generation Xers have been taking over from Baby Boomers as the majority of parents in elementary and secondary education. Gen-X parents and Boomer parents belong to two neighboring generations, each possessing its own location in history and its own peer personality. They are similar in some respects, but clearly different in…

Howe, Neil

2010-01-01

52

Safer Systems: A NextGen Aviation Safety Strategic Goal  

NASA Technical Reports Server (NTRS)

The Joint Planning and Development Office (JPDO), is charged by Congress with developing the concepts and plans for the Next Generation Air Transportation System (NextGen). The National Aviation Safety Strategic Plan (NASSP), developed by the Safety Working Group of the JPDO, focuses on establishing the goals, objectives, and strategies needed to realize the safety objectives of the NextGen Integrated Plan. The three goal areas of the NASSP are Safer Practices, Safer Systems, and Safer Worldwide. Safer Practices emphasizes an integrated, systematic approach to safety risk management through implementation of formalized Safety Management Systems (SMS) that incorporate safety data analysis processes, and the enhancement of methods for ensuring safety is an inherent characteristic of NextGen. Safer Systems emphasizes implementation of safety-enhancing technologies, which will improve safety for human-centered interfaces and enhance the safety of airborne and ground-based systems. Safer Worldwide encourages coordinating the adoption of the safer practices and safer systems technologies, policies and procedures worldwide, such that the maximum level of safety is achieved across air transportation system boundaries. This paper introduces the NASSP and its development, and focuses on the Safer Systems elements of the NASSP, which incorporates three objectives for NextGen systems: 1) provide risk reducing system interfaces, 2) provide safety enhancements for airborne systems, and 3) provide safety enhancements for ground-based systems. The goal of this paper is to expose avionics and air traffic management system developers to NASSP objectives and Safer Systems strategies.

Darr, Stephen T.; Ricks, Wendell R.; Lemos, Katherine A.

2008-01-01

53

Meet Mr. and Mrs. Gen X: A New Parent Generation  

ERIC Educational Resources Information Center

Slowly but surely, Generation Xers have been taking over from Baby Boomers as the majority of parents in elementary and secondary education. In the early 1990s, Gen Xers began joining parent-teacher associations in the nation's elementary schools. Around 2005, they became the majority of middle school parents. By the fall of 2008, they took over…

Howe, Neil

2010-01-01

54

LINK2009 FASE 1 UDVIKLING AF 2. GEN. BRNDSELSCELLE  

E-print Network

LINK2009 FASE 1 »UDVIKLING AF 2. GEN. BR�NDSELSCELLE K�RET�JER OG BRINTTANKSTATION« SLUTRAPPORT: Brændselscelle bil fra LINK2009 til almindeligt bilsyn i Herning #12;LINK2009 FASE 1 SLUTRAPPORT MARTS 2010 Foreningen Hydrogen Link Danmark | www.hydrogenlink.net Side 2 af 56 Indledning & sammendrag I begyndelsen af

55

EnGenIUS -- Environmental Genome Informational Utility System.  

PubMed

Short-insert shotgun sequencing approaches have been applied in recent years to environmental genomic libraries. In the case of complex multispecies microbial communities, there can be many sequence reads that are not incorporated into assemblies, and thus need to be annotated and accessible as single reads. Most existing annotation systems and genome databases accommodate assembled genomes containing contiguous gene-encoding sequences. Thus, a solution is required that can work effectively with environmental genomic annotation information to facilitate data analysis. The Environmental Genome Informational Utility System (EnGenIUS) is a comprehensive environmental genome (metagenome) research toolset that was specifically designed to accommodate the needs of large (> 250 K sequence reads) environmental genome sequencing efforts. The core EnGenIUS modules consist of a set of UNIX scripts and PHP programs used for data preprocessing, an annotation pipeline with accompanying analysis tools, two entity relational databases, and a graphical user interface. The annotation pipeline has a modular structure and can be customized to best fit input data set properties. The integrated entity relational databases store raw data and annotation analysis results. Access to the underlying databases and services is facilitated through a web-based graphical user interface. Users have the ability to browse, upload, download, and analyze preprocessed data, based on diverse search criteria. The EnGenIUS toolset was successfully tested using the Alvinella pompejana epibiont environmental genome data set, which comprises more than 300 K sequence reads. A fully browsable EnGenIUS portal is available at (http://ocean.dbi.udel.edu/) (access code: "guest"). The scope of this paper covers the implementation details and technical aspects of the EnGenIUS toolset. PMID:19090024

Kaplarevic, Mihailo; Murray, Alison E; Cary, Stephen C; Gao, Guang R

2008-12-01

56

Advanced Vehicle Concepts and Implications for NextGen  

NASA Technical Reports Server (NTRS)

This report presents the results of a major NASA study of advanced vehicle concepts and their implications for the Next Generation Air Transportation System (NextGen). Comprising the efforts of dozens of researchers at multiple institutions, the analyses presented here cover a broad range of topics including business-case development, vehicle design, avionics, procedure design, delay, safety, environmental impacts, and metrics. The study focuses on the following five new vehicle types: Cruise-efficient short takeoff and landing (CESTOL) vehicles Large commercial tiltrotor aircraft (LCTRs) Unmanned aircraft systems (UAS) Very light jets (VLJs) Supersonic transports (SST). The timeframe of the study spans the years 2025-2040, although some analyses are also presented for a 3X scenario that has roughly three times the number of flights as today. Full implementation of NextGen is assumed.

Blake, Matt; Smith, Jim; Wright, Ken; Mediavilla Ricky; Kirby, Michelle; Pfaender, Holger; Clarke, John-Paul; Volovoi, Vitali; Dorbian, Christopher; Ashok, Akshay; Reynolds, Tom; Waitz, Ian; Hileman, James; Arunachalam, Sarav; Hedrick, Matt; Vempati, Lakshmi; Laroza, Ryan; denBraven, Wim; Henderson, Jeff

2010-01-01

57

GenNet: A Platform for Hybrid Network Experiments.  

PubMed

We describe General Network (GenNet), a software plugin for the real time experimental interface (RTXI) dynamic clamp system that allows for straightforward and flexible implementation of hybrid network experiments. This extension to RTXI allows for hybrid networks that contain an arbitrary number of simulated and real neurons, significantly improving upon previous solutions that were limited, particularly by the number of cells supported. The benefits of this system include the ability to rapidly and easily set up and perform scalable experiments with hybrid networks and the ability to scan through ranges of parameters. We present instructions for installing, running and using GenNet for hybrid network experiments and provide several example uses of the system. PMID:21845179

Kispersky, Tilman J; Economo, Michael N; Randeria, Pratik; White, John A

2011-01-01

58

J3Gen: a PRNG for low-cost passive RFID.  

PubMed

Pseudorandom number generation (PRNG) is the main security tool in low-cost passive radio-frequency identification (RFID) technologies, such as EPC Gen2. We present a lightweight PRNG design for low-cost passive RFID tags, named J3Gen. J3Gen is based on a linear feedback shift register (LFSR) configured with multiple feedback polynomials. The polynomials are alternated during the generation of sequences via a physical source of randomness. J3Gen successfully handles the inherent linearity of LFSR based PRNGs and satisfies the statistical requirements imposed by the EPC Gen2 standard. A hardware implementation of J3Gen is presented and evaluated with regard to different design parameters, defining the key-equivalence security and nonlinearity of the design. The results of a SPICE simulation confirm the power-consumption suitability of the proposal. PMID:23519344

Melià-Seguí, Joan; Garcia-Alfaro, Joaquin; Herrera-Joancomartí, Jordi

2013-01-01

59

J3Gen: A PRNG for Low-Cost Passive RFID  

PubMed Central

Pseudorandom number generation (PRNG) is the main security tool in low-cost passive radio-frequency identification (RFID) technologies, such as EPC Gen2. We present a lightweight PRNG design for low-cost passive RFID tags, named J3Gen. J3Gen is based on a linear feedback shift register (LFSR) configured with multiple feedback polynomials. The polynomials are alternated during the generation of sequences via a physical source of randomness. J3Gen successfully handles the inherent linearity of LFSR based PRNGs and satisfies the statistical requirements imposed by the EPC Gen2 standard. A hardware implementation of J3Gen is presented and evaluated with regard to different design parameters, defining the key-equivalence security and nonlinearity of the design. The results of a SPICE simulation confirm the power-consumption suitability of the proposal. PMID:23519344

Melià-Seguí, Joan; Garcia-Alfaro, Joaquin; Herrera-Joancomartí, Jordi

2013-01-01

60

Decay heat removal in GEN IV gas cooled fast reactors  

Microsoft Academic Search

The safety goal of the current designs of advanced high-temperature thermal gas-cooled reactors (HTRs) is that no core meltdown would occur in a depressurization event with a combination of concurrent safety system failures. This study focused on the analysis of passive decay heat removal (DHR) in a GEN IV direct-cycle gas-cooled fast reactor (GFR) which is based on the technology

Lap-Yan Cheng; Thomas Y. C. Wei

2009-01-01

61

GenStar: A method for de novo drug design  

Microsoft Academic Search

A novel method, which we call GenStar, has been developed to suggest chemically reasonable structures which fill the active sites of enzymes. The proposed molecules provide good steric contact with the enzyme and exist in low-energy conformations. These structures are composed entirely of sp3 carbons which are grown sequentially, but which can also branch or form rings. User-selected enzyme seed

Sergio H. Rotstein; Mark A. Murcko

1993-01-01

62

Radio Transmission Performance of EPCglobal Gen-2 RFID System  

E-print Network

In this paper, we analyze the performance of the encoding and the modulation processes in the downlink and uplink of the EPCglobal Gen2 system through the analysis and simulation. Furthermore, the synchronization issues on time and frequency domain and the preamble architecture are evaluated. Through the simulation in the uplink, we find that the detection probability of FM0 and Miller coding approaches 1 at 13dB Eb/N0.

Mohaisen, Manar; Chang, KyungHi

2009-01-01

63

PZT piezoelectric films on glass for Gen-X imaging  

Microsoft Academic Search

The proposed adaptive optics system for the Gen-X telescope uses piezoelectric lead zirconate titanate (PZT) films deposited on flexible glass substrates. The low softening transition of the glass substrates imposes several processing challenges that require the development of new approaches to deposit high quality PZT thin films. Synthesis and optimization of chemical solution deposited 1 mum thick films of PbZr0.52Ti0.48O3

Rudeger H. T. Wilke; Susan Trolier-McKinstry; Paul B. Reid; Daniel A. Schwartz

2010-01-01

64

A direct drive permanent magnet generator design for a tidal current turbine(SeaGen)  

Microsoft Academic Search

In this study, the feasibility of a direct-drive per- manent magnet generator for a tidal turbine power take-off system, namely MCT's SeaGen -the world's first full scale commercial tidal turbine- has been investigated. The investigated PM generator topology is called C-GEN which is an air-cored axial-flux generator developed in the University of Edinburgh. The C-GEN is prior to conventional PM

Ozan Keysan; Alasdair S. McDonald; Markus Mueller

2011-01-01

65

Measurement of the neutron electric form factor GEn in quasielastic scattering  

SciTech Connect

We have measured the electric form factor of the neutron, GEn, at two momentum transfers (Q2= 0.5 and Q2= 1.0 GeV/c2) through quasielastic scattering in Jefferson Lab's Hall C. Longitudinally polarized electrons scattered from polarized deuterated ammonia and GEn was extracted from the beam-target asymmetry AVed which, in quasielastic kinematics, is particularly sensitive to GEn and insensitive to MEC and FSI.

Donal Day

2003-07-15

66

Remarks on Peinado et al.'s Analysis of J3Gen.  

PubMed

Peinado et al. analyzed the security of the J3Gen pseudorandom number generator proposed by Melià-Seguí et al., and claimed weaknesses regarding its security properties. They also presented a deterministic attack based on the decimation of the J3Gen output sequences. We show that the assumptions made by Peinado et al. are not correct and that the proposed deterministic attack against J3Gen does not hold in practice. PMID:25781510

Garcia-Alfaro, Joaquin; Herrera-Joancomartí, Jordi; Melià-Seguí, Joan

2015-01-01

67

GEN IV reactors: Where we are, where we should go  

SciTech Connect

GEN IV power plants represent the mid-long term option of the nuclear sector. International literature proposes many papers and reports dealing with these reactors, but there is an evident difference of type and shape of information making impossible each kind of detailed comparison. Moreover, authors are often strongly involved in some particular design; this creates many difficulties in their super-partes position. Therefore it is necessary to put order in the most relevant information to understand strengths and weaknesses of each design and derive an overview useful for technicians and policy makers. This paper presents the state-of the art for GEN IV nuclear reactors providing a comprehensive literature review of the different designs with a relate taxonomy. It presents the more relevant references, data, advantages, disadvantages and barriers to the adoptions. In order to promote an efficient and wide adoption of GEN IV reactors the paper provides the pre-conditions that must be accomplished, enabling factors promoting the implementation and barriers limiting the extent and intensity of its implementation. It concludes outlying the state of the art of the most important R and D areas and the future achievements that must be accomplished for a wide adoption of these technologies. (authors)

Locatelli, G. [Univ. of Lincoln, Lincoln School of Engineering, Brayford Pool - Lincoln LN6 7TS (United Kingdom); Mancini, M.; Todeschini, N. [Politecnico di Milano, Dept. of Management, Economics and Industrial Engineering, Via Lambruschini 4/B, Milano (Italy)

2012-07-01

68

78 FR 54509 - Tenth Meeting: RTCA Next Gen Advisory Committee (NAC)  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF TRANSPORTATION Federal Aviation Administration Tenth Meeting: RTCA Next Gen Advisory Committee (NAC) AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation...

2013-09-04

69

78 FR 5860 - Eighth Meeting: RTCA Next Gen Advisory Committee (NAC)  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF TRANSPORTATION Federal Aviation Administration Eighth Meeting: RTCA Next Gen Advisory Committee (NAC) AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation...

2013-01-28

70

78 FR 28940 - Ninth Meeting: RTCA Next Gen Advisory Committee (NAC)  

Federal Register 2010, 2011, 2012, 2013, 2014

...DEPARTMENT OF TRANSPORTATION Federal Aviation Administration Ninth Meeting: RTCA Next Gen Advisory Committee (NAC) AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation...

2013-05-16

71

Pantanalinema gen. nov. and Alkalinema gen. nov.: novel pseudanabaenacean genera (Cyanobacteria) isolated from saline-alkaline lakes.  

PubMed

The genus Leptolyngbya Anagnostidis & Komárek (1988) was described from a set of strains identified as 'LPP-group B'. The morphology within this group is not particularly informative and underestimates the group's genetic diversity. In the present study, two new pseudanabaenacean genera related to Leptolyngbya morphotypes, Pantanalinema gen. nov. and Alkalinema gen. nov., are described under the provisions of the International Code of Nomenclature for Algae, Fungi and Plants, based on a polyphasic approach. Pantanalinema gen. nov. (type species Pantanalinema rosaneae sp. nov.) has sheaths and trichomes with slight gliding motility, which distinguish this genus from Alkalinema gen. nov. (type species Alkalinema pantanalense sp. nov.), which possesses trichomes arranged in an ornate (interwoven) pattern. 16S rRNA gene sequences of strains of Pantanalinema and Alkalinema exhibited low identity to each other (?91.6?%) and to other sequences from known pseudanabaenacean genera (?94.3 and 93.7?%, respectively). In a phylogenetic reconstruction, six sequences from strains of Pantanalinema and four from strains of Alkalinema formed two separate and robust clades (99?% bootstrap value), with the genera Oculatella and Phormidesmis, respectively, as the closest related groups. 16S-23S rRNA intergenic spacer sequences and secondary structures of strains of Pantanalinema and Alkalinema did not correspond to any previous descriptions. The strains of Pantanalinema and Alkalinema were able to survive and produce biomass at a range of pH (pH 4-11) and were also able to alter the culture medium to pH values ranging from pH 8.4 to 9.9. These data indicate that cyanobacterial communities in underexplored environments, such as the Pantanal wetlands, are promising sources of novel taxa. PMID:25351877

Vieira Vaz, Marcelo Gomes Marçal; Genuário, Diego Bonaldo; Andreote, Ana Paula Dini; Malone, Camila Francieli Silva; Sant'Anna, Célia Leite; Barbiero, Laurent; Fiore, Marli Fátima

2015-01-01

72

Analysis and Improvement of a Pseudorandom Number Generator for EPC Gen2 Tags  

E-print Network

Analysis and Improvement of a Pseudorandom Number Generator for EPC Gen2 Tags J. Melia-Segui1 , J, 08018 Barcelona - Spain, melia@uoc.edu 2 Universitat Aut`onoma de Barcelona, Edifici Q, Campus de-destruction). PRNGs are, therefore, the crucial components that guarantee Gen2 security. #12;2 J. Melia-Segui, J

Garcia-Alfaro, Joaquin

73

CottonGen: a genomics, genetics and breeding database for cotton research  

Technology Transfer Automated Retrieval System (TEKTRAN)

CottonGen (http://www.cottongen.org) is a curated and integrated web-based relational database providing access to publicly available genomic, genetic and breeding data for cotton. CottonGen supercedes CottonDB and the Cotton Marker Database, with enhanced tools for easier data sharing, mining, vis...

74

compreheNGSive: A Tool for Exploring Next-Gen Sequencing Variants Alex Bigelow  

E-print Network

-gen sequencing, biovis, design study 1 INTRODUCTION Next-generation sequencing data is notoriously difficult it is declining sharply, further increasing its abundance [1]. Raw next-generation sequencing (NGS) data must passcompreheNGSive: A Tool for Exploring Next-Gen Sequencing Variants Alex Bigelow University of Utah

Meyer, Miriah

75

75 FR 5780 - Green Borders Geothermal, LLC, Complainant, v. Terra-Gen Dixie Valley, LLC, Respondent; Notice of...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Geothermal, LLC, Complainant, v. Terra-Gen Dixie Valley, LLC, Respondent; Notice...filed a formal complaint against Terra-Gen Dixie Valley, LLC (f/k/a Caithness Dixie Valley, LLC) (Terra-Gen) pursuant to section 206 of the...

2010-02-04

76

76 FR 28973 - Terra-Gen Dixie Valley, LLC; Order on Rehearing and Accepting Tariff Filing, Subject to...  

Federal Register 2010, 2011, 2012, 2013, 2014

...ER11-2127-001, ER11-2127-002, EL11-37-000] Terra-Gen Dixie Valley, LLC; Order on Rehearing and Accepting...transmission tariff (OATT) submitted by Terra-Gen Dixie Valley, LLC (Terra-Gen), in response to a Commission order issued in...

2011-05-19

77

Spatial control of the GEN1 Holliday junction resolvase ensures genome stability  

PubMed Central

Holliday junction (HJ) resolvases are necessary for the processing of persistent recombination intermediates before cell division. Their actions, however, need to be restricted to the late stages of the cell cycle to avoid the inappropriate cleavage of replication intermediates. Control of the yeast HJ resolvase, Yen1, involves phosphorylation changes that modulate its catalytic activity and nuclear import. Here, we show that GEN1, the human ortholog of Yen1, is regulated by a different mechanism that is independent of phosphorylation. GEN1 is controlled exclusively by nuclear exclusion, driven by a nuclear export signal (NES) that restricts GEN1 actions to mitosis when the nuclear membrane breaks down. Construction of a nuclear-localized version of GEN1 revealed that its premature actions partially suppress phenotypes associated with loss of BLM and MUS81, but cause elevated crossover formation. The spatial control of GEN1 therefore contributes to genome stability, by avoiding competition with non-crossover promoting repair pathways. PMID:25209024

Chan, Ying Wai; West, Stephen C.

2014-01-01

78

Meloneis Gen. Nov., a New Epipsammic Genus of Rhaphoneidaceae (Bacillariophyceae)  

PubMed Central

The diatom family Rhaphoneidaceae is characterized by high generic diversity and low species diversity with most genera known to have long stratigraphic ranges. The genera within this family are neritic marine, and mostly epipsammic. A new modern and epipsammic genus, Meloneis gen. nov., is described herein and is compared to all genera within Rhaphoneidaceae and especially to Rhaphoneis Ehrenberg s.l. Within Meloneis three new species and one variety are distinguished and described herein: M. mimallis sp. nov., M. mimallis var. zephyria var. nov., M. akytos sp. nov., and M. gorgis sp. nov. PMID:22442663

Louvrou, Ioanna; Danielidis, Daniel B.; Economou-Amilli, Athena

2012-01-01

79

TelDB: GenLink Multimedia Telomere Resource  

NSDL National Science Digital Library

This TelDB metasite provides information on telomere research and a searchable citations database, including over 1100 telomere-related citations from 200 journals. TelDB is part of GenLink, an NIH-funded resource headed by researchers at Washington University at St. Louis. There has been a recent finding regarding telomerase, a gene which affects the mechanisms controlling human cell replication. The site above provides general information on telomerase, current telomere research, and the use of telemorase in medical practice. Telomerase increases the life-span of a cell, and is thus central to both aging and cancer.

80

GenGIS: A geospatial information system for genomic data  

PubMed Central

The increasing availability of genetic sequence data associated with explicit geographic and ecological information is offering new opportunities to study the processes that shape biodiversity. The generation and testing of hypotheses using these data sets requires effective tools for mathematical and visual analysis that can integrate digital maps, ecological data, and large genetic, genomic, or metagenomic data sets. GenGIS is a free and open-source software package that supports the integration of digital map data with genetic sequences and environmental information from multiple sample sites. Essential bioinformatic and statistical tools are integrated into the software, allowing the user a wide range of analysis options for their sequence data. Data visualizations are combined with the cartographic display to yield a clear view of the relationship between geography and genomic diversity, with a particular focus on the hierarchical clustering of sites based on their similarity or phylogenetic proximity. Here we outline the features of GenGIS and demonstrate its application to georeferenced microbial metagenomic, HIV-1, and human mitochondrial DNA data sets. PMID:19635847

Parks, Donovan H.; Porter, Michael; Churcher, Sylvia; Wang, Suwen; Blouin, Christian; Whalley, Jacqueline; Brooks, Stephen; Beiko, Robert G.

2009-01-01

81

Neoscardovia arbecensis gen. nov., sp. nov., isolated from porcine slurries.  

PubMed

Three Gram-positive, anaerobic, pleomorphic strains (PG10(T), PG18 and PG22), were selected among five strains isolated from pig slurries while searching for host specific bifidobacteria to track the source of fecal pollution in water. Analysis of the 16S rRNA gene sequence showed a maximum identity of 94% to various species of the family Bifidobacteriaceae. However, phylogenetic analyses of 16S rRNA and HSP60 gene sequences revealed a closer relationship of these strains to members of the recently described Aeriscardovia, Parascardovia and Scardovia genera, than to other Bifidobacterium species. The names Neoscardovia gen. nov. and Neoscardovia arbecensis sp. nov. are proposed for a new genus and for the first species belonging to this genus, respectively, and for which PG10(T) (CECT 8111(T), DSM 25737(T)) was designated as the type strain. This new species should be placed in the Bifidobacteriaceae family within the class Actinobacteria, with Aeriscardovia aeriphila being the closest relative. The prevailing cellular fatty acids were C(16:0) and C(18:1)?9c, and the major polar lipids consisted of a variety of glycolipids, diphosphatidyl glycerol, two unidentified phospholipids, and phosphatidyl glycerol. The peptidoglycan structure was A1?meso-Dpm-direct. The GenBank accession numbers for the 16S rRNA gene and HSP60 gene sequences of strains PG10(T), PG18 and PG22 are JF519691, JF519693, JQ767128 and JQ767130, JQ767131, JQ767133, respectively. PMID:22824582

García-Aljaro, Cristina; Ballesté, Elisenda; Rosselló-Móra, Ramon; Cifuentes, Ana; Richter, Michael; Blanch, Anicet R

2012-09-01

82

Next gen wavelets down-sampling preserving statistics  

NASA Astrophysics Data System (ADS)

We extend the 2 nd Gen Discrete Wavelet Transform (DWT) of Swelden to the Next Generations (NG) Digital Wavelet Transform (DWT) preserving the statistical salient features. The lossless NG DWT accomplishes the data compression of "wellness baseline profiles (WBP)" of aging population at homes. For medical monitoring system at home fronts we translate the military experience to dual usage of veterans & civilian alike with the following three requirements: (i) Data Compression: The necessary down sampling reduces the immense amount of data of individual WBP from hours to days and to weeks for primary caretakers in terms of moments, e.g. mean value, variance, etc., without the artifacts caused by FFT arbitrary windowing. (ii) Lossless: our new NG_DWT must preserve the original data sets. (iii) Phase Transition: NG_DWT must capture the critical phase transition of the wellness toward the sickness with simultaneous display of local statistical moments. According to the Nyquist sampling theory, assuming a band-limited wellness physiology, we must sample the WBP at least twice per day since it is changing diurnally and seasonally. Since NG_DWT, like the 2 nd Gen, is lossless, we can reconstruct the original time series for the physicians' second looks. This technique of NG_DWT can also help stock market day-traders monitoring the volatility of multiple portfolios without artificial horizon artifacts.

Szu, Harold; Miao, Lidan; Chanyagon, Pornchai; Cader, Masud

2007-04-01

83

Mutation and association analysis of GEN1 in breast cancer susceptibility  

PubMed Central

GEN1 was recently identified as a key Holliday junction resolvase involved in homologous recombination. Somatic truncating GEN1 mutations have been reported in two breast cancers. Together these data led to the proposition that GEN1 is a breast cancer predisposition gene. In this article we have formally investigated this hypothesis. We performed full-gene mutational analysis of GEN1 in 176 BRCA1/2-negative familial breast cancer samples and 159 controls. We genotyped six SNPs tagging the 30 common variants in the transcribed region of GEN1 in 3,750 breast cancer cases and 4,907 controls. Mutation analysis revealed one truncating variant, c.2515_2519del-AAGTT, which was present in 4% of cases and 4% of controls. We identified control individuals homozygous for the deletion, demonstrating that the last 69 amino acids of GEN1 are dispensable for its function. We identified 17 other variants, but their frequency did not significantly differ between cases and controls. Analysis of 3,750 breast cancer cases and 4,907 controls demonstrated no evidence of significant association with breast cancer for six SNPs tagging the 30 common GEN1 variants. These data indicate that although it also plays a key role in double-strand DNA break repair, GEN1 does not make an appreciable contribution to breast cancer susceptibility by acting as a high- or intermediate-penetrance breast cancer predisposition gene like BRCA1, BRCA2, CHEK2, ATM, BRIP1 and PALB2 and that common GEN1 variants do not act as low-penetrance susceptibility alleles analogous to SNPs in FGFR2. Furthermore, our analyses demonstrate the importance of undertaking appropriate genetic investigations, typically full gene screening in cases and controls together with large-scale case–control association analyses, to evaluate the contribution of genes to cancer susceptibility. PMID:20512659

Turnbull, Clare; Hines, Sarah; Renwick, Anthony; Hughes, Deborah; Pernet, David; Elliott, Anna; Seal, Sheila; Warren-Perry, Margaret; Evans, D. Gareth; Eccles, Diana; Stratton, Michael R.

2013-01-01

84

Mutation and association analysis of GEN1 in breast cancer susceptibility.  

PubMed

GEN1 was recently identified as a key Holliday junction resolvase involved in homologous recombination. Somatic truncating GEN1 mutations have been reported in two breast cancers. Together these data led to the proposition that GEN1 is a breast cancer predisposition gene. In this article we have formally investigated this hypothesis. We performed full-gene mutational analysis of GEN1 in 176 BRCA1/2-negative familial breast cancer samples and 159 controls. We genotyped six SNPs tagging the 30 common variants in the transcribed region of GEN1 in 3,750 breast cancer cases and 4,907 controls. Mutation analysis revealed one truncating variant, c.2515_2519delAAGTT, which was present in 4% of cases and 4% of controls. We identified control individuals homozygous for the deletion, demonstrating that the last 69 amino acids of GEN1 are dispensable for its function. We identified 17 other variants, but their frequency did not significantly differ between cases and controls. Analysis of 3,750 breast cancer cases and 4,907 controls demonstrated no evidence of significant association with breast cancer for six SNPs tagging the 30 common GEN1 variants. These data indicate that although it also plays a key role in double-strand DNA break repair, GEN1 does not make an appreciable contribution to breast cancer susceptibility by acting as a high- or intermediate-penetrance breast cancer predisposition gene like BRCA1, BRCA2, CHEK2, ATM, BRIP1 and PALB2 and that common GEN1 variants do not act as low-penetrance susceptibility alleles analogous to SNPs in FGFR2. Furthermore, our analyses demonstrate the importance of undertaking appropriate genetic investigations, typically full gene screening in cases and controls together with large-scale case-control association analyses, to evaluate the contribution of genes to cancer susceptibility. PMID:20512659

Turnbull, Clare; Hines, Sarah; Renwick, Anthony; Hughes, Deborah; Pernet, David; Elliott, Anna; Seal, Sheila; Warren-Perry, Margaret; Gareth Evans, D; Eccles, Diana; Stratton, Michael R; Rahman, Nazneen

2010-11-01

85

What Can a Historian Do with AstroGen?  

NASA Astrophysics Data System (ADS)

"Astrogen", the Astronomy Genealogy Project, is in the development stage. Patterned after the Mathematics Genealogy Project at http://genealogy.math.ndsu.nodak.edu, it will eventually include most of the world's astronomers, past and present, and provide information about their years of life, highest degrees, universities, and thesis titles. There will also be links to online theses, home pages, and obituaries when these are available. Although a few details remain to be worked out before it becomes public, it is possible to make some use of what has already been compiled. I will give an example, comparing graduates of Harvard University, the University of California at Berkeley, and the University of Chicago from different decades, with information about their professional careers and publication records. The author welcomes queries about AstroGen and is seeking more participants.

Tenn, Joseph S.

2015-01-01

86

Decay heat removal in GEN IV gas cooled fast reactors.  

SciTech Connect

The safety goal of the current designs of advanced high-temperature thermal gas-cooled reactors (HTRs) is that no core meltdown would occur in a depressurization event with a combination of concurrent safety system failures. This study focused on the analysis of passive decay heat removal (DHR) in a GEN IV direct-cycle gas-cooled fast reactor (GFR) which is based on the technology developments of the HTRs. Given the different criteria and design characteristics of the GFR, an approach different from that taken for the HTRs for passive DHR would have to be explored. Different design options based on maintaining core flow were evaluated by performing transient analysis of a depressurization accident using the system code RELAP5-3D. The study also reviewed the conceptual design of autonomous systems for shutdown decay heat removal and recommends that future work in this area should be focused on the potential for Brayton cycle DHRs.

Cheng, L. Y.; Wei, T. Y. C. (Nuclear Engineering Division); (BNL)

2009-08-01

87

GenSensor Suite: A Web-Based Tool for the Analysis of Gene and Protein Interactions, Pathways, and Regulation  

PubMed Central

The GenSensor Suite consists of four web tools for elucidating relationships among genes and proteins. GenPath results show which biochemical, regulatory, or other gene set categories are over- or under-represented in an input list compared to a background list. All common gene sets are available for searching in GenPath, plus some specialized sets. Users can add custom background lists. GenInteract builds an interaction gene list from a single gene input and then analyzes this in GenPath. GenPubMed uses a PubMed query to identify a list of PubMed IDs, from which a gene list is extracted and queried in GenPath. GenViewer allows the user to query one gene set against another in GenPath. GenPath results are presented with relevant P- and q-values in an uncluttered, fully linked, and integrated table. Users can easily copy this table and paste it directly into a spreadsheet or document. PMID:22194743

Gosink, Mark; Khuri, Sawsan; Valdes, Camilo; Jiang, Zhijie; Tsinoremas, Nicholas F.

2011-01-01

88

TheJournalofGeneralPhysiology J. Gen. Physiol. The Rockefeller University Press $30.00  

E-print Network

TheJournalofGeneralPhysiology J. Gen. Physiol. © The Rockefeller University Press $30.00 Volume 130 Lame After All: Kinesin Still Walks with a Hobbled Head Nicholas R. Guydosh1 and Steven M. Block2 1

Block, Steven

89

Notes on GenICode c Miguel Garcia, STS, Hamburg University of Technology  

E-print Network

that GenI- Code can consume define that language fragment, and thus the need to explore what GenICode does wrappers" are used: final case class ARRAY(val elem: TypeKind) extends TypeKind case class BOXED(kind: TypeKind) extends TypeKind final case class REFERENCE(cls: Symbol) extends TypeKind Examples for the REFERENCE

Moeller, Ralf

90

GenIE: an intelligent system for writing genetic counseling patient letters.  

PubMed

We are developing GenIE, a prototype intelligent system to create first drafts of genetic counseling patient letters. GenIE will apply natural language generation techniques to construct the first draft of a letter for subsequent review and editing, if needed, by the genetic counselor. For purposes of knowledge acquisition, we have been analyzing a corpus of patient letters. Based on the corpus analysis we are developing a knowledge base and text generation strategies. PMID:16779256

Green, Nancy

2005-01-01

91

Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov.  

PubMed

A Gram-positive staining, rod-shaped, non-motile, spore-forming obligately anaerobic bacterium, designated CRIBT, was isolated from the gastro-intestinal tract of a rat and characterized. The major cellular fatty acids of strain CRIBT were saturated and unsaturated straight-chain C12-C19 fatty acids, with C16:0 being the predominant fatty acid. The polar lipid profile comprised six glycolipids, four phospholipids and one lipid that did not stain with any of the specific spray reagents used. The only quinone was MK-6. The predominating cell-wall sugars were glucose and galactose. The peptidoglycan type of strain CRIBT was A1? lanthionine-direct. The genomic DNA G+C content of strain CRIBT was 28.1 mol%. On the basis of 16S rRNA gene sequence similarity, strain CRIBT was most closely related to a number of species of the genus Clostridium, including Clostridium lituseburense (97.2%), Clostridium glycolicum (96.2%), Clostridium mayombei (96.2%), Clostridium bartlettii (96.0%) and Clostridium irregulare (95.5%). All these species show very low 16S rRNA gene sequence similarity (<85%) to the type strain of Clostridium butyricum, the type species of the genus Clostridium. DNA-DNA hybridization with closely related reference strains indicated reassociation values below 32%. On the basis of phenotypic and genetic studies, a novel genus, Romboutsia gen. nov., is proposed. The novel isolate CRIBT (=DSM 25109T=NIZO 4048T) is proposed as the type strain of the type species, Romboutsia ilealis gen. nov., sp. nov., of the proposed novel genus. It is proposed that C. lituseburense is transferred to this genus as Romboutsia lituseburensis comb. nov. Furthermore, the reclassification into novel genera is proposed for C. bartlettii, as Intestinibacter bartlettii gen. nov., comb. nov. (type species of the genus), C. glycolicum, as Terrisporobacter glycolicus gen. nov., comb. nov. (type species of the genus), C. mayombei, as Terrisporobacter mayombei gen. nov., comb. nov., and C. irregulare, as Asaccharospora irregularis gen. nov., comb. nov. (type species of the genus), on the basis of additional data collected in this study. In addition, an emendation of the species Peptostreptococcus anaerobius and the order Eubacteriales is provided. PMID:24480908

Gerritsen, Jacoline; Fuentes, Susana; Grievink, Wieke; van Niftrik, Laura; Tindall, Brian J; Timmerman, Harro M; Rijkers, Ger T; Smidt, Hauke

2014-05-01

92

Preliminary Investigation of Civil Tiltrotor in NextGen Airspace  

NASA Technical Reports Server (NTRS)

Presentation intro: Tiltrotor aircraft have long been envisioned as being a potentially viable means of commercial aviation transport. Preliminary results from an ongoing study into the operational and technological considerations of Civil Tiltrotor (CTR) operation in the Next Generation airspace, circa the 2025 time-frame, are presented and discussed. In particular, a fleet of CTR aircraft has been conceptually designed. The performance characteristics of this CTR fleet was subsequently translated into BADA (Base of Aircraft DAta) models that could be used as input to emulate CTR aircraft operations in the ACES and AvTerminal airspace and terminal area simulation tools. A network of nine North-Eastern corridor airports is the focus of the airspace simulation effort; the results from this airport network viII then be extrapolated to provide insights into systemic impact of CTRs on the National Airspace System (NAS). Future work will also be detailed as to attempts to model the systemic effects of noise and emissions from this fleet of new aircraft as well as assess their leveraged impact on public service missions, in time of need, such as major regional/national disaster relief efforts. The ideal outcome of this study is a set of results whereby Next Gen airspace CONOPs can be refined to reflect potential CTR capabilities and, conversely, CTR technology development efforts can be better informed as to key performance requirement thresholds needed to be met in order to successfully introduce these aircraft into civilian aviation operation.

Young, Larry A.; Salvano, Dan; Wright, Ken; Chung, William; Young, Ray; Miller, David; Paris, Alfanso; Gao, Huina; Cheng, Victor

2010-01-01

93

Pisciglobus halotolerans gen. nov., sp. nov., isolated from fish sauce.  

PubMed

Two strains of Gram-stain-positive, catalase-negative, tetrad-forming cocci, C01(T) and C02, were isolated in Thailand from fish sauce. They were facultatively anaerobic, non-motile and non-spore-forming bacteria. These strains produced l-lactic acid from glucose. They grew at pH 5.0-9.0, at 15-40 °C and in the presence of 10?% (w/v) NaCl. The dominant fatty acid was C(18?:?1)?9c. The DNA G+C contents of strains C01(T) and C02 were 38.6 and 38.7 mol%, respectively. Strain C01(T) was related most closely to Desemzia incerta DSM 20581(T), with a 16S rRNA gene sequence similarity of 96.9?%. The strains could be distinguished clearly from D. incerta DSM 20581(T) based on cell morphology, physiological and biochemical characteristics and low levels of DNA-DNA relatedness. On the basis of the data presented, strains C01(T) and C02 are considered to represent a novel species of a new genus in the Bacillus-Lactobacillus cluster, for which the name Pisciglobus halotolerans gen. nov., sp. nov. is proposed. The type strain of Pisciglobus halotolerans is C01(T) (?=?KCTC 13150(T) ?=?TISTR 1958(T) ?=?PCU 316(T)). PMID:20729308

Tanasupawat, Somboon; Thongsanit, Jaruwan; Thawai, Chitti; Lee, Keun Chul; Lee, Jung-Sook

2011-07-01

94

Amylibacter marinus gen. nov., sp. nov., isolated from surface seawater.  

PubMed

A Gram-stain-negative, non-motile, mesophilic, aerobic, rod-shaped bacterium, designated strain 2-3(T), was isolated from surface seawater at Muroto city, Kochi prefecture, Japan. This strain grew well with starch. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the family Rhodobacteraceae and that the strain was related most closely to the genus Pacificibacter (94.0?% sequence similarity to the type strain). The DNA G+C content was 52.4 mol%. The major fatty acids were C18?:?1?7c, C14?:?0 and C16?:?0. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified lipid, one unidentified aminolipid and one unidentified phospholipid. The major isoprenoid quinone was Q-10. Strain 2-3(T) did not grow at 4 or 35 °C, while the type strain of the type species of the genus Pacificibacter grows at both temperatures. From the taxonomic data obtained in this study, it is proposed that strain 2-3(T) be placed into a novel genus and species named Amylibacter marinus gen. nov., sp. nov. in the family Rhodobacteraceae. The type strain of Amylibacter marinus is 2-3(T) (?=?NBRC 110140(T)?=?LMG 28364(T)). PMID:25225261

Teramoto, Maki; Nishijima, Miyuki

2014-12-01

95

Eisenbergiella tayi gen. nov., sp. nov., isolated from human blood.  

PubMed

A catalase-positive, rod-shaped, non-proteolytic, non-motile, anaerobic bacterial strain, designated B086562(T), was isolated from a blood culture of an 84-year-old male patient in Israel. According to 16S rRNA gene sequence phylogeny, this strain has no known close relatives among recognized bacteria but should be placed within the family Lachnospiraceae. The most closely related recognized bacteria were from the 'Clostridium clostridioforme group': C. clostridioforme (92.4%) and Clostridium bolteae (92.3%). The isolate produced butyrate, lactate, acetate and succinate as major metabolic end products. The major fatty acids were C16:0 and C18:1 cis 9 DMA and the DNA G+C content was 46.0 mol%. On the basis of the phenotypic properties and phylogenetic distinctiveness, the blood isolate represents a novel species of a new genus in the family Lachnospiraceae, for which the name Eisenbergiella tayi gen. nov., sp. nov. is proposed. The type strain of Eisenbergiella tayi is B086562(T) (?=?LMG 27400(T)?=?DSM 26961(T)?=?ATCC BAA-2558(T)). PMID:24282142

Amir, Itay; Bouvet, Philippe; Legeay, Christine; Gophna, Uri; Weinberger, Abraham

2014-03-01

96

Oxoid CO2 Gen atmosphere generation system for growth of capnophilic bacteria: an evaluation.  

PubMed

The Oxoid CO2 Gen system is compared with BBL GasPak and a carbon dioxide (CO2) incubator to evaluate its ability to support and enhance the growth of capnophilic bacteria. Clinical samples (n = 109) from various anatomical sites and 23 spiked samples are evaluated. The criteria used to compare the systems include amount of growth, colony size, colony morphology and haemolysis. Isolation rates, amounts of growth and morphology were similar in each system, but colony diameter was significantly larger in the jar-based systems. Significantly larger colonies grew in CO2 Gen than in BBL GasPak. alpha-Haemolytic zones were significantly larger in jar-based systems than in the CO2 incubator, and significantly larger in CO2 Gen than in BBL GasPak. beta-haemolytic zones were significantly larger in CO2 Gen than in the CO2 incubator. The CO2 Gen system appears to be an excellent alternative to established methods for generating an environment for capnophilic incubation. PMID:10824326

Turner, A; Hart, I T

1999-01-01

97

Safety Sufficiency for NextGen: Assessment of Selected Existing Safety Methods, Tools, Processes, and Regulations  

NASA Technical Reports Server (NTRS)

NextGen is a complex socio-technical system and, in many ways, it is expected to be more complex than the current system. It is vital to assess the safety impact of the NextGen elements (technologies, systems, and procedures) in a rigorous and systematic way and to ensure that they do not compromise safety. In this study, the NextGen elements in the form of Operational Improvements (OIs), Enablers, Research Activities, Development Activities, and Policy Issues were identified. The overall hazard situation in NextGen was outlined; a high-level hazard analysis was conducted with respect to multiple elements in a representative NextGen OI known as OI-0349 (Automation Support for Separation Management); and the hazards resulting from the highly dynamic complexity involved in an OI-0349 scenario were illustrated. A selected but representative set of the existing safety methods, tools, processes, and regulations was then reviewed and analyzed regarding whether they are sufficient to assess safety in the elements of that OI and ensure that safety will not be compromised and whether they might incur intolerably high costs.

Xu, Xidong; Ulrey, Mike L.; Brown, John A.; Mast, James; Lapis, Mary B.

2013-01-01

98

Initial Investigation of Operational Concept Elements for NASA's NextGen-Airportal Project Research  

NASA Technical Reports Server (NTRS)

The NextGen-Airportal Project is organized into three research focus areas: Safe and Efficient Surface Operations, Coordinated Arrival/Departure Operations Management, and Airportal Transition and Integration Management. The content in this document was derived from an examination of constraints and problems at airports for accommodating future increases in air traffic, and from an examination of capabilities envisioned for NextGen. The concepts are organized around categories of constraints and problems and therefore do not precisely match, but generally reflect, the research focus areas. The concepts provide a framework for defining and coordinating research activities that are, and will be, conducted by the NextGen-Airportal Project. The concepts will help the research activities function as an integrated set focused on future needs for airport operations and will aid aligning the research activities with NextGen key capabilities. The concepts are presented as concept elements with more detailed sub-elements under each concept element. For each concept element, the following topics are discussed: constraints and problems being addressed, benefit descriptions, required technology and infrastructure, and an initial list of potential research topics. Concept content will be updated and more detail added as the research progresses. The concepts are focused on enhancing airportal capacity and efficiency in a timeframe 20 to 25 years in the future, which is similar to NextGen's timeframe.

Lohr, Gary; Lee, Jonathan; Poage, James L.; Tobias, Leonard

2009-01-01

99

Evaluation of AnaeroGen system for growth of anaerobic bacteria.  

PubMed Central

The Oxoid AnaeroGen system was compared with the BBL GasPak for the production of an anaerobic atmosphere and was evaluated for its ability to support the growth of 135 clinically significant anaerobic bacteria. An anaerobe chamber was used as the "gold standard" for supporting the growth of anaerobes. The AnaeroGen requires no catalyst, produces no hydrogen, requires no water, and reduces preparation time to a minimum. The water-activated BBL GasPak generates hydrogen. For 132 of the 135 strains tested, better initial growth at 48 h was noted for the jar methods than for the anaerobe chamber. At 72 h, 113 of the 135 strains showed equal growth, and at 7 days, only marginal differences in growth patterns were noted. The AnaeroGen never failed to reduce the anaerobic indicator, while the BBL GasPak occasionally failed to do so. The AnaeroGen performed at least as well as, and sometimes better than, the established methods. The AnaeroGen is a good alternative for use in anaerobic jars. PMID:7494033

Miller, P H; Wiggs, L S; Miller, J M

1995-01-01

100

GEN | News Highlights: Scientists Develop High-Capacity Nanoparticles for Targeted Delivery of Drug Cocktails http://www.genengnews.com/gen-news-highlights/scientists-develop-high-capacity-nanoparticles-for-targeted-delivery-of-drug-cocktails/81245016/[4/  

E-print Network

GEN | News Highlights: Scientists Develop High-Capacity Nanoparticles for Targeted Delivery of Drug Cocktails http://www.genengnews.com/gen-news-highlights/scientists-develop-high-capacity-nanoparticles-for-targeted Magazine CurrentIssue View Larger Image Past Issues Adlink Subscription ADVERTISEMENT Comments Email

Brinker, C. Jeffrey

101

A Rapid Micro Polymerase Chain Reaction System (GenSpector Micro PCR) for Hepatitis B Virus DNA Detection  

E-print Network

A Rapid Micro Polymerase Chain Reaction System (GenSpector® Micro PCR) for Hepatitis B Virus DNA a rapid micro PCR (polymerase chain reaction) system (GenSpector® Micro PCR) for the application of Technology, PO Box 111, Suwon 440-600, Korea Abstract This paper presents a rapid micro PCR (polymerase chain

Oh, Kwang W.

102

USDA Forest Service Gen. Tech. Rep. PSW-GTR-181. 2002. 717 Diameters and Heights of Trees with  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-GTR-181. 2002. 717 Diameters and Heights of Trees where trees are smaller, as in the spruce-willow-birch zone of British Columbia (Bunnell and others 1999@intergate.ca) #12;Diameters and Heights of Cavity Trees--Bunnell, Wind, Boyland, and Houde USDA Forest Service Gen

Standiford, Richard B.

103

Complete cDNAs from Nylanderia cf. pubens (Hymenoptera: Formicidae). --GenBank accession numbers: JF815100-JF815104  

Technology Transfer Automated Retrieval System (TEKTRAN)

5 new gene sequences were identified from workers of Caribbean crazy ant, Nylanderia cf. pubens, and submitted to the National Center for Biotechnology Information GenBank. GenBank accession numbers are JF815100-JF815104. This information will provide scientists with genetic tools to study the popu...

104

USDA Forest Service Gen. Tech. Rep. PSW-GTR-193. 2004. 33 Interannual-scale to Century-scale Climate  

E-print Network

Service Gen. Tech. Rep. PSW-GTR-193. 2004.34 The Texture of Climate Variability in the Sierra NevadaUSDA Forest Service Gen. Tech. Rep. PSW-GTR-193. 2004. 33 Interannual-scale to Century-scale Climate Variability in Western North America1 Malcolm K. Hughes2 "All our direct observations are from

Standiford, Richard B.

105

75 FR 1052 - Terra-Gen Dixie Valley, LLC; TGP Dixie Development Company, LLC; New York Canyon, LLC; Notice of...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Regulatory Commission [Docket No. EL10-29-000] Terra-Gen Dixie Valley, LLC; TGP Dixie Development Company, LLC; New...30, 2009. Take notice that on December 24, 2009, Terra-Gen Dixie Valley, LLC, TGP Dixie Development Company, LLC...

2010-01-08

106

Discourses in Conflict: The Relationship between Gen Y Pre-Service Teachers, Digital Technologies and Lifelong Learning  

ERIC Educational Resources Information Center

This paper analyses Gen Y pre-service primary school teachers' conceptualisations of lifelong learning. It is situated within a context of improving the provision and delivery of pre-service teacher education. This paper argues that Gen Y's understanding of lifelong learning has been influenced by their engagements with digital technologies and…

Donnison, Sharn

2009-01-01

107

Complete cDNAs from Nylanderia sp. nr. pubens (Hymenoptera: Formicidae). GenBank GU980916-GU980928.  

Technology Transfer Automated Retrieval System (TEKTRAN)

13 new gene sequences were identified from workers of Rasberry crazy ant, Nylanderia sp.nr. pubens, and submitted to the National Center for Biotechnology Information GenBank. GenBank accession numbers are GU980916-GU980928. This information will provide scientists with genetic tools to study the p...

108

Complete cDNAs from Solenopsis invicta (Hymenoptera: Formicidae). --GenBank accession numbers: HM130684-HM130685.  

Technology Transfer Automated Retrieval System (TEKTRAN)

2 new gene sequences were identified from workers of Solenopsis invicta, and submitted to the National Center for Biotechnology Information GenBank. GenBank accession numbers are HM130684-HM130685. This information will provide scientists with genetic tools to study the populations of this ant....

109

Genetic Extended-Fiber Network (GEN) Stretched Over Microbridges Fabricated by Single-Mask Inclined UV Lithography  

Microsoft Academic Search

In this study, we propose a Genetic Extended-fiber Network (GEN) separately stretched over microbridges for DNA fiber applications. The microbridges are rapidly and easily fabricated by directly spin-coating a thick negative photoresist on a metal mask patterned glass substrate and exposing it twice from the backside of the substrate for two different exposure angles. The GEN constructed over microbridge array

Takaaki Suzuki; Daisuke Hiramaru; Ariko Fuke; Isaku Kanno; Hiroyuki Kabata; Hidetoshi Kotera

2007-01-01

110

Critical Issues on Materials for Gen-IV Reactors  

SciTech Connect

Within the LDRD on 'Critical Issues on Materials for Gen-IV Reactors' basic thermodynamics of the Fe-Cr alloy and accurate atomistic modeling were used to help develop the capability to predict hardening, swelling and embrittlement using the paradigm of Multiscale Materials Modeling. Approaches at atomistic and mesoscale levels were linked to build-up the first steps in an integrated modeling platform that seeks to relate in a near-term effort dislocation dynamics to polycrystal plasticity. The requirements originated in the reactor systems under consideration today for future sources of nuclear energy. These requirements are beyond the present day performance of nuclear materials and calls for the development of new, high temperature, radiation resistant materials. Fe-Cr alloys with 9-12% Cr content are the base matrix of advanced ferritic/martensitic (FM) steels envisaged as fuel cladding and structural components of Gen-IV reactors. Predictive tools are needed to calculate structural and mechanical properties of these steels. This project represents a contribution in that direction. The synergy between the continuous progress of parallel computing and the spectacular advances in the theoretical framework that describes materials have lead to a significant advance in our comprehension of materials properties and their mechanical behavior. We took this progress to our advantage and within this LDRD were able to provide a detailed physical understanding of iron-chromium alloys microstructural behavior. By combining ab-initio simulations, many-body interatomic potential development, and mesoscale dislocation dynamics we were able to describe their microstructure evolution. For the first time in the case of Fe-Cr alloys, atomistic and mesoscale were merged and the first steps taken towards incorporating ordering and precipitation effects into dislocation dynamics (DD) simulations. Molecular dynamics (MD) studies of the transport of self-interstitial, vacancy and point defect clusters in concentrated Fe-Cr alloys were performed for future diffusion data calculations. A recently developed parallel MC code with displacement allowed us to predict the evolution of the defect microstructures, local chemistry changes, grain boundary segregation and precipitation resulting from radiation enhanced diffusion. We showed that grain boundaries, dislocations and free surfaces are not preferential for alpha-prime precipitation, and explained experimental observations of short-range order (SRO) in Fe-rich FeCr alloys. Our atomistic studies of dislocation hardening allowed us to obtain dislocation mobility functions for BCC pure iron and Fe-Cr and determine for FCC metals the dislocation interaction with precipitates with a description to be used in Dislocation Dynamic (DD) codes. A Synchronous parallel Kinetic Monte Carlo code was developed and tested which promises to expand the range of applicability of kMC simulations. This LDRD furthered the limits of the available science on the thermodynamic and mechanic behavior of metallic alloys and extended the application of physically-based multiscale materials modeling to cases of severe temperature and neutron fluence conditions in advanced future nuclear reactors. The report is organized as follows: after a brief introduction, we present the research activities, and results obtained. We give recommendations on future LLNL activities that may contribute to the progress in this area, together with examples of possible research lines to be supported.

Caro, M; Marian, J; Martinez, E; Erhart, P

2009-02-27

111

Falsochrobactrum ovis gen. nov., sp. nov., isolated from a sheep.  

PubMed

A Gram-stain-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (B1315(T)) was isolated from the placenta of a sheep with abortion. On the basis of 16S rRNA gene sequence analyses the strain was assigned to the Brucella-Ochrobactrum-Paenochrobactrum-Pseudochrobactrum group with 94.5-94.8?%, 94.3-96.1?%, 95.0-95.1?%, and 95.9-96.1?% sequence similarities to type strains of species of the four genera, respectively. Phylogenetic trees indicated a close relationship to the type strains of Ochrobactrum gallinifaecis and Ochrobactrum oryzae (95.9 and 96.1?% sequence similarity, respectively). Chemotaxonomic data confirmed the allocation of strain B1315(T) to the family Brucellaceae (quinone system: ubiquinone Q-10 and major fatty acids: C18?:?1?7c and C19?:?0 cyclo ?8c). The polar lipid profile contained the major lipids diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and the unidentified but unique phospholipid PL7. The polyamine pattern of strain B1315(T) contained the major polyamines spermidine and putrescine. On the basis of the 16S rRNA gene and recA sequence phylogeny and chemotaxonomic data strain B1315(T) was clearly different from the genera Brucella, Ochrobactrum, Paenochrobactrum and Pseudochrobactrum. On the basis of these data we propose the novel genus Falsochrobactrum gen. nov. with the type species Falsochrobactrum ovis sp. nov. with the type strain B1315(T) (?=?CCM 8460(T)?=?LMG 27356(T.)) The taxonomic allocation of O. gallinifaecis, which grouped inconsistently together with strain B1315(T) on the basis of 16S rRNA gene sequence data, but shows the chemotaxonomic features of the genus Ochrobactrum, remains to be clarified. PMID:23687057

Kämpfer, Peter; Glaeser, Stefanie; Busse, Hans-Jürgen; Eisenberg, Tobias; Scholz, Holger

2013-10-01

112

Chungangia koreensis gen. nov., sp. nov., isolated from marine sediment.  

PubMed

A Gram-staining-positive, strictly aerobic, non-spore-forming, rod-shaped bacterial strain, CAU 9163(T), was isolated from marine sediment collected in the Republic of Korea and its taxonomic position was investigated using a polyphasic approach. The novel strain grew optimally at 30 °C and pH 8.0. In phylogenetic analysis based on 16S rRNA gene sequences, strain CAU 9163(T) formed a hitherto unknown lineage within the order Bacillales, which contains the genera Planomicrobium, Planococcus, Sporosarcina, Rummeliibacillus, Viridibacillus, Lysinibacillus and Bacillus. The levels of 16S rRNA gene sequence similarity between the novel strain and any established bacterial species were all <95.7%. The major isoprenoid quinines of strain CAU 9163(T) were MK-8 (65.2%) and MK-7 (22.8%) and the predominant fatty acid was anteiso-C(15:0). The peptidoglycan was of the A4? type and based on L-Lys-D-Asp. The major whole-cell sugars were ribose and glucose. The polar lipid profile mainly consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified polar lipid. The genomic DNA G+C content of the novel strain was 44.3 mol%. These data were sufficient to differentiate the novel strain from established genera in the phylum Firmicutes. Based on the phenotypic, chemotaxonomic and genotypic evidence, strain CAU 9163(T) represents a novel species in a new genus for which the name Chungangia koreensis gen. nov., sp. nov. is proposed. The type strain of Chungangia koreensis is 9163(T) (?=?KCTC 13729(T) ?=?CCUG 59778(T)). PMID:21984680

Kim, Wonyong; Traiwan, Jitsopin; Park, Mi-Hak; Jung, Min Young; Oh, Su-Jin; Yoon, Jung-Hoon; Sukhoom, Ampaitip

2012-08-01

113

Motiliproteus sediminis gen. nov., sp. nov., isolated from coastal sediment.  

PubMed

A novel Gram-stain-negative, rod-to-spiral-shaped, oxidase- and catalase- positive and facultatively aerobic bacterium, designated HS6(T), was isolated from marine sediment of Yellow Sea, China. It can reduce nitrate to nitrite and grow well in marine broth 2216 (MB, Hope Biol-Technology Co., Ltd) with an optimal temperature for growth of 30-33 °C (range 12-45 °C) and in the presence of 2-3% (w/v) NaCl (range 0.5-7%, w/v). The pH range for growth was pH 6.2-9.0, with an optimum at 6.5-7.0. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that the novel isolate was 93.3% similar to the type strain of Neptunomonas antarctica, 93.2% to Neptunomonas japonicum and 93.1% to Marinobacterium rhizophilum, the closest cultivated relatives. The polar lipid profile of the novel strain consisted of phosphatidylethanolamine, phosphatidylglycerol and some other unknown lipids. Major cellular fatty acids were summed feature 3 (C(16:1) ?7c/iso-C15:0 2-OH), C(18:1) ?7c and C(16:0) and the main respiratory quinone was Q-8. The DNA G+C content of strain HS6(T) was 61.2 mol%. Based on the phylogenetic, physiological and biochemical characteristics, strain HS6(T) represents a novel genus and species and the name Motiliproteus sediminis gen. nov., sp. nov., is proposed. The type strain is HS6(T) (=ATCC BAA-2613(T)=CICC 10858(T)). PMID:25038887

Wang, Zong-Jie; Xie, Zhi-Hong; Wang, Chao; Du, Zong-Jun; Chen, Guan-Jun

2014-10-01

114

Allobacillus halotolerans gen. nov., sp. nov. isolated from shrimp paste.  

PubMed

A novel bacterial strain designated B3A(T), isolated from shrimp paste, was investigated by a polyphasic taxonomic approach. Cells stained Gram-positive and were aerobic, non-pigmented, sporulating and rod-shaped with a polar flagellum. 16S rRNA gene sequence analysis indicated that strain B3A(T) belonged to the class Bacilli and was a member of the family Bacillaceae. Strain B3A(T) shared low levels of 16S rRNA gene sequence similarity (<94.0 %) with members of other genera in the family Bacillaceae and was most closely related to Halalkalibacillus halophilus BH2(T) (93.8 % sequence similarity). The isolate was able to grow at 20-45 °C, with 0.5-15 % NaCl and at pH 6-9. Menaquinone with seven isoprene units (MK-7) was the major respiratory quinone and 16 : 0 iso, 15 : 0 anteiso and 14 : 0 iso were the major fatty acids. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The characteristic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The DNA G+C content was 45.3 mol%. On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic and physiological data, strain B3A(T) represents a novel genus and species in the family Bacillaceae for which the name Allobacillus halotolerans gen. nov., sp. nov. is proposed. The type strain of Allobacillus halotolerans is B3A(T) (?=?BCRC 17939(T) ?=?LMG 24826(T)). PMID:20511460

Sheu, Shih-Yi; Arun, A B; Jiang, Sing-Rong; Young, Chiu-Chung; Chen, Wen-Ming

2011-05-01

115

75 FR 27332 - AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC; Eagle Creek Water Resources, LLC; Eagle Creek Land...  

Federal Register 2010, 2011, 2012, 2013, 2014

...10481-064, and 9690-106] AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC...7, 2010. On April 30, 2010, AER NY-Gen, LLC (transferor) and Eagle Creek Hydro...transferor: Mr. Joseph Klimaszewski, AER NY- Gen, LLC, 613 Plank Road, Forestburgh,...

2010-05-14

116

77 FR 13592 - AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC, Eagle Creek Land...  

Federal Register 2010, 2011, 2012, 2013, 2014

...Commission [Project No. 10482-107] AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC...Intervene On February 24, 2012, AER NY-Gen, LLC (transferor), Eagle Creek Hydro...Transferor: Mr. Joseph Klimaszewski, AER NY- Gen, LLC, P.O. Box 876, East...

2012-03-07

117

Tools for Designing, Evaluating, and Certifying NextGen Technologies and Procedures: Automation Roles and Responsibilities  

NASA Technical Reports Server (NTRS)

Barbara Kanki from NASA Ames Research Center will discuss research that focuses on the collaborations between pilots, air traffic controllers and dispatchers that will change in NextGen systems as automation increases and roles and responsibilities change. The approach taken by this NASA Ames team is to build a collaborative systems assessment template (CSAT) based on detailed task descriptions within each system to establish a baseline of the current operations. The collaborative content and context are delineated through the review of regulatory and advisory materials, policies, procedures and documented practices as augmented by field observations and interviews. The CSAT is developed to aid the assessment of key human factors and performance tradeoffs that result from considering different collaborative arrangements under NextGen system changes. In theory, the CSAT product may be applied to any NextGen application (such as Trajectory Based Operations) with specified ground and aircraft capabilities.

Kanki, Barbara G.

2011-01-01

118

Magnetic behavior in Cr2@Gen (1?n?12) clusters: A density functional investigation  

NASA Astrophysics Data System (ADS)

With a goal to produce magnetic moment in Cr2 Doped Gen clusters which will be useful for practical applications, we have considered the structure and magnetic properties of Pure Germanium clusters and substitutionally doped it with Cr dimer to produce Cr2@Gen clusters. As the first step of calculation, geometrical optimizations of the nanoclusters have been done. These optimized geometries have been used in calculate the average binding energy per atom (BE), HOMO-LUMO gap and hence the relative stability of the clusters. These parameters have been demonstrated as structural and electronic properties of the clusters. Gap between highest occupied molecular orbital and lowest unoccupied molecular orbital indicate cluster to be a potential motif for generating magnetic cluster assembled materials. Based on these values a comparative study on different sized clusters has been done in order to understand the origin of structures, electronic and magnetic properties of Cr2@Gen nanoclusters.

Dhaka, Kapil; Trivedi, Ravi; Bandyopadhyay, Debashis

2014-04-01

119

Halobellus clavatus gen. nov., sp. nov. and Halorientalis regularis gen. nov., sp. nov., two new members of the family Halobacteriaceae.  

PubMed

Four halophilic archaeal strains, designated TNN18(T), TBN12, TNN28(T) and TBN19, were isolated from brines sampled from two artificial marine solar salterns in eastern China. Strains TNN18(T) and TNN28(T) were isolated from the Tainan marine solar saltern, whereas strains TBN12 and TBN19 were from the Taibei marine solar saltern. Colonies of the four strains were red-pigmented and their cells were pleomorphic, motile, Gram-reaction-negative rods. Strains TNN18(T) and TBN12 were able to grow at 25-50 °C (optimum 37 °C), in 10-3 ?% (w/v) NaCl (optimum 15 %), with 0-1.0 M MgCl(2) (optimum 0.05 M) and at pH 5.5-9.0 (optimum pH 7.0-7.5), while strains TNN28(T) and TBN19 were able to grow at 20-50 °C (optimum 37 °C), in 15-30 % (w/v) NaCl (optimum 18-20 %), in 0.005-1.0 M MgCl(2) (optimum 0.01-0.3 M) and at pH 6.0-9.0 (optimum pH 7.0-7.5). Cells of these strains lyse in distilled water; minimal NaCl concentrations to prevent cell-lysis are 10?% (w/v) for strains TNN18(T) and TBN12 and 12 % (w/v) for strains TNN28(T) and TBN19. The major polar lipids of strains TNN18(T) and TBN12 were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS) and one major glycolipid (GL1), which was chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). Minor amounts of other lipids (GL0, GL2, GL3 and GL4) were also detectable. The polar lipid profiles of strains TNN28(T) and TBN19 contained PG, PGP-Me, GL1, which was chromatographically identical to S-DGD-1, and three to four minor unidentified glycolipids (GL2-GL5). Phylogenetic analyses revealed that strains TNN18(T) and TBN12 formed a distinct clade with strains of the closest related species, Haloquadratum walsbyi (91.5-91.8 % 16S rRNA gene sequence similarity) and strains TNN28(T) and TBN19 formed a distinct clade with strains of the species Halosimplex carlsbadense (89.9-93.3 % similarity) and two members of the genus Halorhabdus (92.5-93.3 % similarity). The DNA G+C contents of strains TNN18(T), TBN12, TNN28(T) and TBN19 were 61.5, 62.4, 61.9 and 61.5 mol%, respectively. DNA-DNA hybridization values between strains TNN18(T) and TBN12, and strains TNN28(T) and TBN19 were 82.9 % and 88.2 %, respectively. The phenotypic, chemotaxonomic and phylogenetic properties suggest that the four strains represent two novel species of two new genera within the family Halobacteriaceae, for which the names Halobellus clavatus gen. nov., sp. nov. (type strain TNN18(T ) = CGMCC 1.10118(T ) = JCM 16424(T)) and Halorientalis regularis gen. nov., sp. nov. (type strain TNN28(T ) = CGMCC 1.10123(T ) = JCM 16425(T)) are proposed. PMID:21169458

Cui, Heng-Lin; Yang, Xin; Gao, Xia; Xu, Xue-Wei

2011-11-01

120

Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; Hallella seregens gen. nov., sp. nov.; Johnsonella ignava gen. nov., sp. nov.; and Dialister pneumosintes gen. nov., comb. nov., nom. rev., Anaerobic gram-negative bacilli from the human gingival crevice.  

PubMed

The following four new species of anaerobic gram-negative bacilli isolated from the human gingival crevice are described: Oribaculum catoniae, with ATCC 51270 as the type strain; Catonella morbi, with ATCC 51271 as the type strain; Hallella seregens, with ATCC 51272 as the type strain; and Johnsonella ignava, with ATCC 51276 as the type strain. C. morbi is associated with periodontitis. H. seregens and J. ignava are associated with gingivitis and periodontitis. O. catoniae has been isolated from healthy and diseased gingiva. Dialister pneumosintes (Olitsky and Gates 1921) gen. nov., comb. nov., nom. rev., associated with gingivitis, is proposed to accommodate organisms formerly classified as Bacteroides pneumosintes. PMID:8186083

Moore, L V; Moore, W E

1994-04-01

121

Batwanema gen. n. and Chokwenema gen. n. (Oxyurida, Hystrignathidae), new nematode genera as parasites of Passalidae (Coleoptera) from the Democratic Republic of Congo.  

PubMed

Two new genera and species parasitizing passalid beetles from the Democratic Republic of Congo are described. Batwanema congo gen. n. et sp. n. is characterized by having females with the cervical cuticle armed with scale-like projections, arranged initially in rows of eight elements that gradually divide and form pointed spines toward the end of the spiny region, two cephalic annuli, clavate procorpus and genital tract monodelphic-prodelphic. Two Malagasian species of Artigasia Christie, 1934 were placed in this genus as B. latum (Van Waerebeke, 1973) comb. n. and B. annulatum (Van Waerebeke, 1973) comb. n. Chokwenema lepidophorum gen. n. et sp. n. is characterized by having females with the cervical cuticle armed with scale-like projections, arranged initially in rows of eight elements (similar to Batwanema) that divide gradually, forming spines; a single cephalic annule cone-like, truncated, moderately inflated; procorpus sub-cylindrical and genital tract didelphic-amphidelphic. PMID:24363593

Morffe, Jans; García, Nayla

2013-01-01

122

Batwanema gen. n. and Chokwenema gen. n. (Oxyurida, Hystrignathidae), new nematode genera as parasites of Passalidae (Coleoptera) from the Democratic Republic of Congo  

PubMed Central

Abstract Two new genera and species parasitizing passalid beetles from the Democratic Republic of Congo are described. Batwanema congo gen. n. et sp. n. is characterized by having females with the cervical cuticle armed with scale-like projections, arranged initially in rows of eight elements that gradually divide and form pointed spines toward the end of the spiny region, two cephalic annuli, clavate procorpus and genital tract monodelphic-prodelphic. Two Malagasian species of Artigasia Christie, 1934 were placed in this genus as B. latum (Van Waerebeke, 1973) comb. n. and B. annulatum (Van Waerebeke, 1973) comb. n. Chokwenema lepidophorum gen. n. et sp. n. is characterized by having females with the cervical cuticle armed with scale-like projections, arranged initially in rows of eight elements (similar to Batwanema) that divide gradually, forming spines; a single cephalic annule cone-like, truncated, moderately inflated; procorpus sub-cylindrical and genital tract didelphic-amphidelphic. PMID:24363593

Morffe, Jans; García, Nayla

2013-01-01

123

NextGen Operations in a Simulated NY Area Airspace  

NASA Technical Reports Server (NTRS)

A human-in-the-loop simulation conducted in the Airspace Operations Laboratory (AOL) at NASA Ames Research Center explored the feasibility of a Next Generation Air Transportation System (NextGen) solution to address airspace and airport capacity limitations in and around the New York metropolitan area. A week-long study explored the feasibility of a new Optimal Profile Descent (OPD) arrival into the airspace as well as a novel application of a Terminal Area Precision Scheduling and Spacing (TAPSS) enhancement to the Traffic Management Advisor (TMA) arrival scheduling tool to coordinate high volume arrival traffic to intersecting runways. In the simulation, four en route sector controllers and four terminal radar approach control (TRACON) controllers managed traffic inbound to Newark International Airport's primary runway, 22L, and its intersecting overflow runway, 11. TAPSS was used to generate independent arrival schedules for each runway and a traffic management coordinator participant adjusted the arrival schedule for each runway 11 aircraft to follow one of the 22L aircraft. TAPSS also provided controller-managed spacing tools (slot markers with speed advisories and timelines) to assist the TRACON controllers in managing the arrivals that were descending on OPDs. Results showed that the tools significantly decreased the occurrence of runway violations (potential go-arounds) when compared with a Baseline condition with no tools. Further, the combined use of the tools with the new OPD produced a peak arrival rate of over 65 aircraft per hour using instrument flight rules (IFR), exceeding the current maximum arrival rate at Newark Liberty International Airport (EWR) of 52 per hour under visual flight rules (VFR). Although the participants rated the workload as relatively low and acceptable both with and without the tools, they rated the tools as reducing their workload further. Safety and coordination were rated by most participants as acceptable in both conditions, although the TRACON Runway Coordinator (TRC) rated neither as acceptable in the Baseline condition. Regarding the role of the TRC, the two TRACON controllers handling the 11 arrivals indicated that the TRC was very much needed in the Baseline condition without tools, but not needed in the condition with tools. This indicates that the tools were providing much of the sequencing and spacing information that the TRC had supplied in the Baseline condition.

Smith, Nancy M.; Parke, Bonny; Lee, Paul; Homola, Jeff; Brasil, Connie; Buckley, Nathan; Cabrall, Chris; Chevalley, Eric; Lin, Cindy; Morey, Susan; Omar, Faisal; Rein-Weston, Daphne; Yoo, Hyo-Sang

2013-01-01

124

PZT piezoelectric films on glass for Gen-X imaging  

NASA Astrophysics Data System (ADS)

The proposed adaptive optics system for the Gen-X telescope uses piezoelectric lead zirconate titanate (PZT) films deposited on flexible glass substrates. The low softening transition of the glass substrates imposes several processing challenges that require the development of new approaches to deposit high quality PZT thin films. Synthesis and optimization of chemical solution deposited 1 ?m thick films of PbZr0.52Ti0.48O3 on small area (1 in2) and large area (16 in2) Pt/Ti/glass substrates has been performed. In order to avoid warping of the glass at temperatures typically used to crystallize PZT films ({700°C), a lower temperature, two-step crystallization process was employed. An {80 nm thick seed layer of PbZr0.30Ti0.70O3 was deposited to promote the growth of the perovskite phase. After the deposition of the seed layer, the films were annealed in a rapid thermal annealing (RTA) furnace at 550°C for 3 minutes to nucleate the perovskite phase. This was followed by isothermal annealing at 550°C for 1 hour to complete crystallization. For the subsequent PbZr0.52Ti0.48O3 layers, the same RTA protocol was performed, with the isothermal crystallization implemented following the deposition of three PbZr0.52Ti0.48O3 spin-coated layers. Over the frequency range of 1 kHz to 100 kHz, films exhibit relative permittivity values near 800 with loss tangents below 0.07. Hysteresis loops show low levels of imprint with coercive fields of 40-50 kV/cm in the forward direction and 50-70 kV/cm in the reverse direction. The remanent polarization varied from 25-35 ?C/cm2 and e31,f values were approximately -5.0 C/m2. In scaling up the growth procedure to large area films, where warping becomes more pronounced due to the increased size of the substrate, the pyrolysis and crystallization conditions were performed in a box furnace to improve the temperature uniformity. By depositing films on both sides of the glass substrate, the tensile stresses are balanced, providing a sufficiently flat surface to continue PZT deposition. The properties of the large area film are comparable to those obtained on small substrates. While sol-gel processing is a viable approach to the deposition of high quality PZT thin films on glass substrates, preliminary results using RF magnetron sputter deposition demonstrate comparable properties with a significantly simpler process that offers a superior route for large scale production.

Wilke, Rudeger H. T.; Trolier-McKinstry, Susan; Reid, Paul B.; Schwartz, Daniel A.

2010-09-01

125

Overview of NASA's Next Generation Air Transportation System (NextGen) Research  

NASA Technical Reports Server (NTRS)

This slide presentation is an overview of the research for the Next Generation Air Transportation System (NextGen). Included is a review of the current air transportation system and the challenges of air transportation research. Also included is a review of the current research highlights and significant accomplishments.

Swenson, Harry N.

2009-01-01

126

CottonGen: a genomics, genetics and breeding database for cotton research.  

PubMed

CottonGen (http://www.cottongen.org) is a curated and integrated web-based relational database providing access to publicly available genomic, genetic and breeding data for cotton. CottonGen supercedes CottonDB and the Cotton Marker Database, with enhanced tools for easier data sharing, mining, visualization and data retrieval of cotton research data. CottonGen contains annotated whole genome sequences, unigenes from expressed sequence tags (ESTs), markers, trait loci, genetic maps, genes, taxonomy, germplasm, publications and communication resources for the cotton community. Annotated whole genome sequences of Gossypium raimondii are available with aligned genetic markers and transcripts. These whole genome data can be accessed through genome pages, search tools and GBrowse, a popular genome browser. Most of the published cotton genetic maps can be viewed and compared using CMap, a comparative map viewer, and are searchable via map search tools. Search tools also exist for markers, quantitative trait loci (QTLs), germplasm, publications and trait evaluation data. CottonGen also provides online analysis tools such as NCBI BLAST and Batch BLAST. PMID:24203703

Yu, Jing; Jung, Sook; Cheng, Chun-Huai; Ficklin, Stephen P; Lee, Taein; Zheng, Ping; Jones, Don; Percy, Richard G; Main, Dorrie

2014-01-01

127

Targeted amplicon sequencing (TAS): a scalable next-gen approach to multilocus, multitaxa phylogenetics.  

PubMed

Next-gen sequencing technologies have revolutionized data collection in genetic studies and advanced genome biology to novel frontiers. However, to date, next-gen technologies have been used principally for whole genome sequencing and transcriptome sequencing. Yet many questions in population genetics and systematics rely on sequencing specific genes of known function or diversity levels. Here, we describe a targeted amplicon sequencing (TAS) approach capitalizing on next-gen capacity to sequence large numbers of targeted gene regions from a large number of samples. Our TAS approach is easily scalable, simple in execution, neither time-nor labor-intensive, relatively inexpensive, and can be applied to a broad diversity of organisms and/or genes. Our TAS approach includes a bioinformatic application, BarcodeCrucher, to take raw next-gen sequence reads and perform quality control checks and convert the data into FASTA format organized by gene and sample, ready for phylogenetic analyses. We demonstrate our approach by sequencing targeted genes of known phylogenetic utility to estimate a phylogeny for the Pancrustacea. We generated data from 44 taxa using 68 different 10-bp multiplexing identifiers. The overall quality of data produced was robust and was informative for phylogeny estimation. The potential for this method to produce copious amounts of data from a single 454 plate (e.g., 325 taxa for 24 loci) significantly reduces sequencing expenses incurred from traditional Sanger sequencing. We further discuss the advantages and disadvantages of this method, while offering suggestions to enhance the approach. PMID:22002916

Bybee, Seth M; Bracken-Grissom, Heather; Haynes, Benjamin D; Hermansen, Russell A; Byers, Robert L; Clement, Mark J; Udall, Joshua A; Wilcox, Edward R; Crandall, Keith A

2011-01-01

128

WWith new research predicting a signifi-cant chance of a massive, tsunami-gen-  

E-print Network

WWith new research predicting a signifi- cant chance of a massive, tsunami-gen- erating earthquake, taking the message of tsunami and earthquake prepared- ness to community meetings and work- shops up of the fault. Given that the last tsunami-generating quake to hit the Oregon coast occurred in 1700, we

Tullos, Desiree

129

USDA Forest Service Gen. Tech. Rep. RMRS-GTR-42-vol. 6. 2008 61 Introduction ____________________  

E-print Network

bioregionwashistoricallyclosed-canopyconiferousand deciduous forest. Coniferous forests are characterized by spruce (Picea spp, P. resinosa, P. rigida, P. banksi- ana)inthenortheasterncoastalandGreatLakesareas. Deciduous forestsUSDA Forest Service Gen. Tech. Rep. RMRS-GTR-42-vol. 6. 2008 61 Introduction

130

DynGenPar A Dynamic Generalized Parser for Common Mathematical Language  

E-print Network

DynGenPar ­ A Dynamic Generalized Parser for Common Mathematical Language Kevin Kofler and Arnold Neumaier University of Vienna, Austria Faculty of Mathematics Nordbergstr. 15, 1090 Wien, Austria kevin-term goal is to computerize a large library of existing mathematical knowl- edge using the new parser

Neumaier, Arnold

131

225USDA Forest Service Gen. Tech. Rep. PSW-GTR-160. 1997. Constraints on Germination and  

E-print Network

225USDA Forest Service Gen. Tech. Rep. PSW-GTR-160. 1997. Constraints on Germination and Emergence, soil source, and microclimate on viability, germination, emergence, and seedling size of Emory oak increased germination up to 5-fold. Germination did not decline between 0 and 35 days of storage. Acorn size

Standiford, Richard B.

132

Bdellovibrio bacteriovorus gen. et sp. n., a predatory, ectoparasitic, and bacteriolytic microorganism  

Microsoft Academic Search

Bdellovibrio bacteriovorus, gen. et sp. n., a predatory and ectoparasitic microorganism with lytic activity against susceptible bacteria, is described, as are techniques for isolation and cultivation. These unusual bacteria cause reactions that are similar in their outward manifestations to bacteriophage-induced lysis. Upon plating a mixture of host bacteria and parasites, confluent lysis or single plaque formation occurs, just as in

H. Stolp; M. P. Starr

1963-01-01

133

GWAS in a Box: Statistical and Visual Analytics of Structured Associations via GenAMap  

PubMed Central

With the continuous improvement in genotyping and molecular phenotyping technology and the decreasing typing cost, it is expected that in a few years, more and more clinical studies of complex diseases will recruit thousands of individuals for pan-omic genetic association analyses. Hence, there is a great need for algorithms and software tools that could scale up to the whole omic level, integrate different omic data, leverage rich structure information, and be easily accessible to non-technical users. We present GenAMap, an interactive analytics software platform that 1) automates the execution of principled machine learning methods that detect genome- and phenome-wide associations among genotypes, gene expression data, and clinical or other macroscopic traits, and 2) provides new visualization tools specifically designed to aid in the exploration of association mapping results. Algorithmically, GenAMap is based on a new paradigm for GWAS and PheWAS analysis, termed structured association mapping, which leverages various structures in the omic data. We demonstrate the function of GenAMap via a case study of the Brem and Kruglyak yeast dataset, and then apply it on a comprehensive eQTL analysis of the NIH heterogeneous stock mice dataset and report some interesting findings. GenAMap is available from http://sailing.cs.cmu.edu/genamap. PMID:24905018

Xing, Eric P.; Curtis, Ross E.; Schoenherr, Georg; Lee, Seunghak; Yin, Junming; Puniyani, Kriti; Wu, Wei; Kinnaird, Peter

2014-01-01

134

Xylochloris irregularis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga  

E-print Network

green alga JIR I´ NEUSTUPA 1 *, MAREK ELIA´ S1 , PAVEL SKALOUD 1 , YVONNE NE MCOVA´ 1 AND LENKA irregularis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga. Phycologia 50: 57­66. DOI: 10.2216/08-64.1 The phylogenetic diversity of subaerial coccoid green algae remains

135

NASA/CR2012-217553 Safety Assurance in NextGen  

E-print Network

March 2012 NASA/CR­2012-217553 Safety Assurance in NextGen Cody Harrison Fleming, Melissa Spencer Aerospace Advanced Technology, Columbia, Maryland #12;NASA STI Program . . . in Profile Since its founding, NASA has been dedicated to the advancement of aeronautics and space science. The NASA scientific

Leveson, Nancy

136

Leptochlorella corticola gen. et sp. nov. and Kalinella apyrenoidosa sp. nov.: two novel  

E-print Network

Leptochlorella corticola gen. et sp. nov. and Kalinella apyrenoidosa sp. nov.: two novel Chlorella algal genus, Chlorella, which accommodated coccoid unicellular green algal species with globular to oval. These algae share the general Chlorella-like morphology and their 18S rRNA and rbcL gene sequences place them

137

Thaumamermis cosgrovei n. gen., n. sp. (Mermithidae: Nematoda) parasitizing terrestrial isopods (Isopoda: Oniscoidea)  

Microsoft Academic Search

Summary  A new mermithid nematode, Thaumamermis cosgrovei n. gen., n. sp. (Mermithidae: Nematoda) was found parasitizing two terrestrial isopods (Isopoda: Oniscoidea) in California. The hosts, Armadillidium vulgare (Latr.) (a pillbug) and Porcellio scaber (Latr.) (a sowbug) represent the first cases of isopods attacked by mermithid nematodes. The genus Thaumamermis can be distinguished from all previously described mermithids by the extremely dimorphic

George O. Poinar

1981-01-01

138

GenTree: An Interactive Genetic Algorithms System for Designing 3D Polygonal Tree Models  

E-print Network

, designers must become skilled in the use of a commercial modeling program such as 3D Studio Max or MayaGenTree: An Interactive Genetic Algorithms System for Designing 3D Polygonal Tree Models Clare@andrew.cmu.edu Abstract. The creation of individual 3D models to include within a virtual world can be a time

Congdon, Clare Bates

139

Visualizing and Sharing Results in Bioinformatics Projects: GBrowse and GenBank Exports  

Technology Transfer Automated Retrieval System (TEKTRAN)

Effective tools for presenting and sharing data are necessary for collaborative projects, typical for bioinformatics. In order to facilitate sharing our data with other genomics, molecular biology, and bioinformatics researchers, we have developed software to export our data to GenBank and combined ...

140

GenSession: a Flexible Zoomable User Interface for Melody Generation  

E-print Network

. Clips may be freely positioned within a 2D workspace, allowing the user to group or position them of the prototype. The 2D workspace displays a network of interrelated settings objects and clips. GenSession also to reposition them on the 2D workspace, our prototype supports "automatic re-framing", with the

McGuffin, Michael J.

141

TEMPORARY CHANGE REQUEST TCR NO.TCR-GEN-006, R10-002  

E-print Network

TEMPORARY CHANGE REQUEST TCR NO.TCR-GEN-006, R10-002 (e.g., TCR-ENG-021,R0-001) The Temporary Change Request (TCR) Form is to be used to process urgent or minor changes for PPPL Policies, Organization/Mission Statements and Procedures. The TCR should be used when changes are: 1) urgent, and can

Princeton Plasma Physics Laboratory

142

Plant MetGenMAP: an integrative analysis system for plant systems biology  

Technology Transfer Automated Retrieval System (TEKTRAN)

We have developed a web-based system, Plant MetGenMAP, which can identify significantly altered biochemical pathways and highly affected biological processes, predict functional roles of pathway genes, and potential pathway-related regulatory motifs from transcript and metabolite profile datasets. P...

143

SIMILASCAROPHIS N. GEN. N. SPP. (NEMATODA: CYSTIDICOLIDAE) PARASITIZING MARINE FISHES OFF THE CHILEAN COAST  

Microsoft Academic Search

Similascarophis (Cystidicolidae) n. gen. is proposed. In the mouth of specimens of this genus, submedial labia are absent and pseudolabia do not have any part projecting toward the central oral opening. These nematodes were obtained from the alimentary tract of 7 marine fish species along the coast of Chile: Bovichthys chilensis Regan, Eleginops maclovinus(Cuvier), Pinguipes chilensis (Valenciennes), Cilus gilberti (Abbott),

Gabriela Muñoz; María Teresa González; Mario George-Nascimento

2004-01-01

144

Clasificacin de las amenazas a la seguridad en sistemas RFID -EPC Gen2  

E-print Network

Clasificación de las amenazas a la seguridad en sistemas RFID - EPC Gen2 Joan Melià-Seguí, Joaquin este artículo una vista rápida a la familia de amenazas a las que se enfrenta la tecnología EPC. Index las amenazas de seguridad. Como sucede en otras tecnologías emergentes, la falta de seguridad y las

Garcia-Alfaro, Joaquin

145

Two new Stratiomyinae, including Panamamyia gen. nov., from the Neotropical Region (Diptera: Stratiomyidae)  

Technology Transfer Automated Retrieval System (TEKTRAN)

A new species of Hoplitimyia James, H. inbioensis sp. nov., is described from Costa Rica. A new genus, Panamamyia gen. nov., with type species P. silbergliedi sp. nov., is described from Panama. Both taxa are very rare in collections, not having been taken during extensive Malaise trap surveys in ...

146

Balancing Formative and Summative Science Assessment Practices: Year One of the GenScope Assessment Project.  

ERIC Educational Resources Information Center

This paper describes the GenScope Assessment Project, a project that is exploring ways of using multimedia computers to teach complex science content, refining sociocultural views of assessment and motivation, and considering different ways of reconciling the differences between these newer views and prior behavioral and cognitive views. The…

Hickey, Daniel T.; Kruger, Ann Cale; Fredrick, Laura D.; Schafer, Nancy Jo; Kindfield, Ann C. H.

147

Febraury 2011 EA Report Brown Bagger 1 When Gen X is in Charge  

E-print Network

great leaders know how to balance people and processes for the company's overall good. Open dialog style for the company's best inter- ests. After all, if these young leaders don't have the right- tion of grooming Gen-Xers for future leader- ship roles within your organization or you're suddenly

Oliver, Douglas L.

148

Preliminary Results From High Temperature Scoping Irradiation Experiments Of Selected Gen IV Structural Metallic Materials  

Microsoft Academic Search

The performance of Generation IV reactors as a class will be determined by the behavior of advanced engineering materials. In the case of materials utilized for reactor internals and pressure vessels, the effects of irradiation are major issues. The environmental conditions for most of the Gen IV reactors are generally beyond present day reactor technology, especially as regards the combinations

Randy K Nanstad; David A McClintock; David T Hoelzer

2007-01-01

149

Hylodesmus singaporensis gen. et sp. nov., a new autosporic subaerial green alga (Scenedesmaceae,  

E-print Network

Hylodesmus singaporensis gen. et sp. nov., a new autosporic subaerial green alga (Scenedesmaceae characterization of an autosporic coccoid green alga isolated from decaying wood in a natural forest in Singapore. Depending on culture conditions, this alga formed globular to irregularly oval solitary cells

150

USDA Forest Service Gen. Tech. Rep. PSW-110. 1989. 305 SESSION G: WILDLIFE II: MANAGING WILDLIFE  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-110. 1989. 305 SESSION G: WILDLIFE II: MANAGING WILDLIFE as having the greatest biological diversity and the highest productivity among wildlife habitats. Riparian invertebrate, fish, amphibian, reptile, bird, and mammal species. A significant number of wildlife species

Standiford, Richard B.

151

The security of EPC Gen2 compliant RFID Mike Burmester1  

E-print Network

interoperable RFID protocols. It supports efficient tag reading, flexible bandwidth use, multiple readThe security of EPC Gen2 compliant RFID protocols Mike Burmester1 and Breno de Medeiros2 1 tags in the RFID industry. EPCGen2 supports only very basic security tools such as a 16-bit Pseudo

Burmester, Mike

152

PhD Studentships for the Thalis Program "GEnAge": The role of Genetic and  

E-print Network

, there is an immense pressure to identify strategies to prevent or delay age-associated frailty and diseasePhD Studentships for the Thalis Program "GEnAge": The role of Genetic and Environmental Factors in Aging and Longevity With an ever growing proportion of elderly people in Western societies

153

Working with Gen Y Teachers: Dealing with a Changing Teacher Workforce  

ERIC Educational Resources Information Center

A significant change is coming to American classrooms. As Baby Boomer teachers retire and are replaced by members of Generation Y (born between 1977 and 1995) there is growing evidence that they come from a far different set of experiences, experiences that will shape their lives and their careers (Coggins, 2008). Members of Gen Y share several…

Williamson, Ronald; Meyer-Looze, Catherine

2010-01-01

154

A Possible Genome To Architecture Project (GenToA) [The Meta-Genome Project?  

E-print Network

concern about the Human Genome project, comparing it with buying a book written in a language nobodyA Possible Genome To Architecture Project (GenToA) [The Meta-Genome Project?] Installed: 2 Aug 2010 can a genome specify an information-processing architecture that grows itself guided by interaction

Sloman, Aaron

155

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 313 Marbled Murrelet Populations of Washington--  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 313 Marbled Murrelet Populations of Washington Eldridge, Bellingham, Washington 98225 Abstract: Marbled Murrelets occur in Puget Sound marine habitats data indicate that Marbled Murrelet abundance in Puget Sound is now lower than earlier this century

Standiford, Richard B.

156

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 255 Abstract: Marbled Murrelets (Brachyramphus marmoratus) vary  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 255 Abstract: Marbled Murrelets (Brachyramphus of kleptoparasitism. Marbled Murrelets (Brachyramphus marmoratus) occupy a variety of foraging habitats and vary of other seabird species. Because marbled murrelets may be subject to kleptoparasitism in these mixed

Standiford, Richard B.

157

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 49 Nesting Chronology Of The Marbled Murrelet  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 49 Nesting Chronology Of The Marbled Murrelet, and fledgling Marbled Murrelets (Brachyramphus marmoratus) for which the fledging date could be estimated September, a breeding period of 182 days. Detailed information on the breeding chronology of the Marbled

Standiford, Richard B.

158

Drainage fracture networks in elastic solids with internal fluid gen-Maya Kobchenko1  

E-print Network

epl draft Drainage fracture networks in elastic solids with internal fluid gen- eration Maya layer to fracture. The gas produced is drained on short length scales by diffusion and on long length scales by flow in a fracture network, which has topological properties that are intermediate between

Galland, Olivier

159

Tubulideres seminoli gen. et sp. nov. and Zelinkaderes brightae sp. nov. (Kinorhyncha, Cyclorhagida) from Florida  

Microsoft Academic Search

One new kinorhynch genus and species and one new species from the genus Zelinkaderes are described from sandy sediment off Fort Pierce, Florida. The new genus and species, Tubulideres seminoli gen. et sp. nov. is characterized by the presence of the first trunk segment consisting of a closed ring, the second segment\\u000a of a bent tergal plate with a midventral

Martin V. Sørensen; Iben Heiner; Ole Ziemer; Birger Neuhaus

2007-01-01

160

CottonGen: a genomics, genetics and breeding database for cotton research  

PubMed Central

CottonGen (http://www.cottongen.org) is a curated and integrated web-based relational database providing access to publicly available genomic, genetic and breeding data for cotton. CottonGen supercedes CottonDB and the Cotton Marker Database, with enhanced tools for easier data sharing, mining, visualization and data retrieval of cotton research data. CottonGen contains annotated whole genome sequences, unigenes from expressed sequence tags (ESTs), markers, trait loci, genetic maps, genes, taxonomy, germplasm, publications and communication resources for the cotton community. Annotated whole genome sequences of Gossypium raimondii are available with aligned genetic markers and transcripts. These whole genome data can be accessed through genome pages, search tools and GBrowse, a popular genome browser. Most of the published cotton genetic maps can be viewed and compared using CMap, a comparative map viewer, and are searchable via map search tools. Search tools also exist for markers, quantitative trait loci (QTLs), germplasm, publications and trait evaluation data. CottonGen also provides online analysis tools such as NCBI BLAST and Batch BLAST. PMID:24203703

Yu, Jing; Jung, Sook; Cheng, Chun-Huai; Ficklin, Stephen P.; Lee, Taein; Zheng, Ping; Jones, Don; Percy, Richard G.; Main, Dorrie

2014-01-01

161

GenGIS 2: geospatial analysis of traditional and genetic biodiversity, with new gradient algorithms and an extensible plugin framework.  

PubMed

GenGIS is free and open source software designed to integrate biodiversity data with a digital map and information about geography and habitat. While originally developed with microbial community analyses and phylogeography in mind, GenGIS has been applied to a wide range of datasets. A key feature of GenGIS is the ability to test geographic axes that can correspond to routes of migration or gradients that influence community similarity. Here we introduce GenGIS version 2, which extends the linear gradient tests introduced in the first version to allow comprehensive testing of all possible linear geographic axes. GenGIS v2 also includes a new plugin framework that supports the development and use of graphically driven analysis packages: initial plugins include implementations of linear regression and the Mantel test, calculations of alpha-diversity (e.g., Shannon Index) for all samples, and geographic visualizations of dissimilarity matrices. We have also implemented a recently published method for biomonitoring reference condition analysis (RCA), which compares observed species richness and diversity to predicted values to determine whether a given site has been impacted. The newest version of GenGIS supports vector data in addition to raster files. We demonstrate the new features of GenGIS by performing a full gradient analysis of an Australian kangaroo apple data set, by using plugins and embedded statistical commands to analyze human microbiome sample data, and by applying RCA to a set of samples from Atlantic Canada. GenGIS release versions, tutorials and documentation are freely available at http://kiwi.cs.dal.ca/GenGIS, and source code is available at https://github.com/beiko-lab/gengis. PMID:23922841

Parks, Donovan H; Mankowski, Timothy; Zangooei, Somayyeh; Porter, Michael S; Armanini, David G; Baird, Donald J; Langille, Morgan G I; Beiko, Robert G

2013-01-01

162

Environmental Information for the U.S. Next Generation Air Transportation System (NextGen)  

NASA Astrophysics Data System (ADS)

It is estimated that weather is responsible for approximately 70% of all air traffic delays and cancellations in the United States. Annually, this produces an overall economic loss of nearly 40B. The FAA and NASA have determined that weather impacts and other environmental constraints on the U.S. National Airspace System (NAS) will increase to the point of system unsustainability unless the NAS is radically transformed. A Next Generation Air Transportation System (NextGen) is planned to accommodate the anticipated demand for increased system capacity and the super-density operations that this transformation will entail. The heart of the environmental information component that is being developed for NextGen will be a 4-dimensional data cube which will include a single authoritative source comprising probabilistic weather information for NextGen Air Traffic Management (ATM) systems. Aviation weather constraints and safety hazards typically comprise meso-scale, storm-scale and microscale observables that can significantly impact both terminal and enroute aviation operations. With these operational impacts in mind, functional and performance requirements for the NextGen weather system were established which require significant improvements in observation and forecasting capabilities. This will include satellite observations from geostationary and/or polar-orbiting hyperspectral sounders, multi-spectral imagers, lightning mappers, space weather monitors and other environmental observing systems. It will also require improved in situ and remotely sensed observations from ground-based and airborne systems. These observations will be used to better understand and to develop forecasting applications for convective weather, in-flight icing, turbulence, ceilings and visibility, volcanic ash, space weather and the environmental impacts of aviation. Cutting-edge collaborative research efforts and results from NASA, NOAA and the FAA which address these phenomena are summarized. In 2003, a Joint Planning and Development Office (JPDO) was established by public law to meet the significant challenges that NextGen presents. JPDO partners were chartered which include, but are not limited to, the Federal Aviation Administration (FAA), the National Oceanic and Atmospheric Administration (NOAA), the National Aeronautics and Space Administration (NASA), the Department of Defense (DOD) and broad elements of academia and the aviation industry. This paper provides the aviation meteorology community with useful insight on salient NextGen environmental information requirements that have been developed by the JPDO Weather Working Group's Environmental Information Team. These efforts will help to define observation and forecast systems needed to support NextGen and to develop the operational applications for NextGen aviation weather information. Another major goal of this paper is to inform the international weather community of our research progress and plans for NextGen, to foster research collaboration with our colleagues, and to exchange information to maximize success of NextGen, SESAR and related initiatives world-wide.

Murray, J.; Miner, C.; Pace, D.; Minnis, P.; Mecikalski, J.; Feltz, W.; Johnson, D.; Iskendarian, H.; Haynes, J.

2009-09-01

163

Mercury: Next-gen Data Analysis and Annotation Pipeline (Seventh Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting 2012)  

ScienceCinema

David Sexton (Baylor) gives a talk titled "Mercury: Next-gen Data Analysis and Annotation Pipeline" at the 7th Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held in June, 2012 in Santa Fe, NM.

Sexton, David [Baylor

2013-01-25

164

Mercury: Next-gen Data Analysis and Annotation Pipeline (Seventh Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting 2012)  

SciTech Connect

David Sexton (Baylor) gives a talk titled "Mercury: Next-gen Data Analysis and Annotation Pipeline" at the 7th Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held in June, 2012 in Santa Fe, NM.

Sexton, David [Baylor

2012-06-01

165

GenThes: A General Thesaurus Browser for Web-based Catalogue Systems - A Step towards Component based Catalogue Systems  

Microsoft Academic Search

Thesauri have been proven means to identify documents in libraries for centuries. In this paper, we show how this approach can be combined with most recent Internet technologies. The Java-based general thesaurus browser GenThes is able to handle several heterogeneous, multilingual thesauri. With the General European Multilingual Environmental Thesaurus (GEMET), GenThes is currently being used with several environmental catalogue systems.

Ralf Nikolai; Ralf Kramer; Marc Steinhaus

166

Identification and Characterization of Key Human Performance Issues and Research in the Next Generation Air Transportation System (NextGen)  

NASA Technical Reports Server (NTRS)

This report identifies key human-performance-related issues associated with Next Generation Air Transportation System (NextGen) research in the NASA NextGen-Airspace Project. Four Research Focus Areas (RFAs) in the NextGen-Airspace Project - namely Separation Assurance (SA), Airspace Super Density Operations (ASDO), Traffic Flow Management (TFM), and Dynamic Airspace Configuration (DAC) - were examined closely. In the course of the research, it was determined that the identified human performance issues needed to be analyzed in the context of NextGen operations rather than through basic human factors research. The main gaps in human factors research in NextGen were found in the need for accurate identification of key human-systems related issues within the context of specific NextGen concepts and better design of the operational requirements for those concepts. By focusing on human-system related issues for individual concepts, key human performance issues for the four RFAs were identified and described in this report. In addition, mixed equipage airspace with components of two RFAs were characterized to illustrate potential human performance issues that arise from the integration of multiple concepts.

Lee, Paul U.; Sheridan, Tom; Poage, james L.; Martin, Lynne Hazel; Jobe, Kimberly K.

2010-01-01

167

Towards Structuring Unstructured GenBank Metadata for Enhancing Comparative Biological Studies  

PubMed Central

Within large sequence repositories such as GenBank there is a wealth of metadata providing contextual information that may enhance search and retrieval of relevant sequences for a range of subsequent analyses. One challenge is the use of free-text in these metadata fields where approaches are needed to extract, structure, and encode essential information. The goal of the present study was to explore the feasibility of using a combination of existing resources for annotating unstructured GenBank metadata, initially focusing on the “host” and “isolation_source” fields. This paper summarizes early results for 10 host organisms that include a characterization of associated isolation sources with respect to biomedical ontologies and semantic types. The findings from this preliminary study provide insights to the rich amount of information captured within these unstructured metadata, guidance for addressing the challenges and issues encountered, and highlight the potential value for enriching comparative biological studies towards improving human health. PMID:22211174

Chen, Elizabeth S.; Sarkar, Indra Neil

2011-01-01

168

Tadaridanema delicatus (Schwartz, 1927) n. gen., n. comb. (Trichostrongylina: Molineidae) parasite of Molossidae bats.  

PubMed

On the basis of the revision of the type material of Anoplostrongylus delicatus Schwartz, 1927, and new specimens collected from Tadarida brasiliensis mexicana (Saussure, 1860) in 4 arid localities from Mexico, we describe a new genus (Tadaridanema n. gen.), to which A. delicatus is transferred (as Tadaridanema delicatus (Schwartz, 1927) n. gen., n. comb.). This new genus differs from all other genera included in Anoplostrongylinae by having ray 2 larger than ray 3. In addition, T. delicatus can be differentiated from the type species of Anoplostrongylus (Anoplostrongylus paradoxus (Travassos, 1918)) because it possess vestibular branches equal in length, cephalic inflation divided into 2 regions, and synlophe with many small ridges at the midbody level, whereas in T. delicatus, vestibular branches are equal in size, cephalic inflation is simple in structure, and the synlophe has only 2 well-developed dorsal cuticular ridges. PMID:17152947

Falcón-Ordaz, Jorge; Guzmán-Cornejo, Carmen; García-Prieto, Luis; Gardner, Scott Lyell

2006-10-01

169

Parametric Modeling of the Safety Effects of NextGen Terminal Maneuvering Area Conflict Scenarios  

NASA Technical Reports Server (NTRS)

The goal of this work was to analytically identify and quantify the issues, challenges, technical hurdles, and pilot-vehicle interface issues associated with conflict detection and resolution (CD&R)in emerging operational concepts for a NextGen terminal aneuvering area, including surface operations. To this end, the work entailed analytical and trade studies focused on modeling the achievable safety benefits of different CD&R strategies and concepts in the current and future airport environment. In addition, crew-vehicle interface and pilot performance enhancements and potential issues were analyzed based on review of envisioned NextGen operations, expected equipage advances, and human factors expertise. The results of perturbation analysis, which quantify the high-level performance impact of changes to key parameters such as median response time and surveillance position error, show that the analytical model developed could be useful in making technology investment decisions.

Rogers, William H.; Waldron, Timothy P.; Stroiney, Steven R.

2011-01-01

170

Gen 2.0 Mixer/Ejector Nozzle Test at LSAF June 1995 to July 1996  

NASA Technical Reports Server (NTRS)

Testing of the HSCT Generation 2.0 nozzle model hardware was conducted at the Boeing Low Speed Aeroacoustic Facility, LSAF. Concurrent measurements of noise and thrust were made at critical takeoff design conditions for a variety of mixer/ejector model hardware. Design variables such as suppressor area ratio, mixer area ratio, liner type and thickness, ejector length, lobe penetration, and mixer chute shape were tested. Parallel testing was conducted at G.E.'s Cell 41 acoustic free jet facility to augment the LSAF test. The results from the Gen 2.0 testing are being used to help shape the current nozzle baseline configuration and guide the efforts in the upcoming Generation 2.5 and 3.0 nozzle tests. The Gen 2.0 results have been included in the total airplane system studies conducted at MDC and Boeing to provide updated noise and thrust performance estimates.

Arney, L. D.; Sandquist, D. L.; Forsyth, D. W.; Lidstone, G. L.; Long-Davis, Mary Jo (Technical Monitor)

2005-01-01

171

TALN 2008, Avignon, 913 juin 2008 E-Gen : Profilage automatique de candidatures  

E-print Network

TALN 2008, Avignon, 9­13 juin 2008 E-Gen : Profilage automatique de candidatures Rémy Kessler1, 84911 Avignon (2) AKTOR 12, allée Irène Joliot Curie 69800 Saint Priest {remy.kessler, juan motivation et curriculum vitae). #12;Kessler, Torres-Moreno and El-Bèze. Nos précédents travaux (Kessler et

Avignon et des Pays de Vaucluse, Université de

172

Jackie Lee, Ted Selker, Elliott Precher, Rob Gens MIT Media Lab  

E-print Network

Physical Actions Introduction Connecting to 3D CAD software, like 3DS Max or Rhino, isphere is able + - 5V MAX475 10k 100k 0.1uf 0.1uf Hands Sensors Hardware iSphere is a 3D input device that can response,selker,elliottp,gens}@media.mit.edu iSphere: A Proximity-based 3D Input Device Questions to Jackie Lee, jackylee@media.mit.edu SIGGRAPH

173

Dual-track CCS stakeholder engagement: Lessons learned from FutureGen in Illinois  

USGS Publications Warehouse

FutureGen, as originally planned, was to be the world's first coal-fueled, near-zero emissions power plant with fully integrated, 90% carbon capture and storage (CCS). From conception through siting and design, it enjoyed strong support from multiple stakeholder groups, which benefited the overall project. Understanding the stakeholder engagement process for this project provides valuable insights into the design of stakeholder programs for future CCS projects. FutureGen is one of few projects worldwide that used open competition for siting both the power plant and storage reservoir. Most site proposals were coordinated by State governments. It was unique in this and other respects relative to the site selection method used on other DOE-supported projects. At the time of site selection, FutureGen was the largest proposed facility designed to combine an integrated gasification combined cycle (IGCC) coal-fueled power plant with a CCS system. Stakeholder engagement by states and the industry consortium responsible for siting, designing, building, and operating the facility took place simultaneously and on parallel tracks. On one track were states spearheading state-wide site assessments to identify candidate sites that they wanted to propose for consideration. On the other track was a public-private partnership between an industry consortium of thirteen coal companies and electric utilities that comprised the FutureGen Alliance (Alliance) and the U.S. Department of Energy (DOE). The partnership was based on a cooperative agreement signed by both parties, which assigned the lead for siting to the Alliance. This paper describes the stakeholder engagement strategies used on both of these tracks and provides examples from the engagement process using the Illinois semi-finalist sites. ?? 2011 Published by Elsevier Ltd.

Hund, G.; Greenberg, S.E.

2011-01-01

174

Enhancing Security of EPCglobal Gen2 RFID Tag against Traceability and Cloning  

Microsoft Academic Search

In this paper, we present a synchronization-based communication protocol for RFID devices. We focus on the EPCGlobal Class-1 Gen-2 RFID tag which supports only simple cryptographic primitives like Pseudo-random Number Generator (PRNG) and Cyclic Redundancy Code (CRC). Our protocol is secure in a sense that it prevents the cloned tags and malicious readers from impersonating and abusing legitimate tags, respectively.

Dang Nguyen Duc; PARK Jaemin; Hyunrok Lee; Kwangjo Kim

2006-01-01

175

Emergency Decay Heat Removal in a GEN-IV Gas-Cooled Fast Reactor  

Microsoft Academic Search

A series of transient analyses using the system code RELAP5-3d has been performed to confirm the efficacy of a proposed hybrid active\\/passive combination approach to the decay heat removal for an advanced 2400 MWt GEN-IV gas-cooled fast reactor. The accident sequence of interest is a station blackout simultaneous with a small break (10 sq.inch\\/0.645 m²) in the reactor vessel. The

Lap Y. Cheng; Hans Ludewig; Jae Jo

2006-01-01

176

Bias explorer: measurements of compositional bias in EMBL and GenBank sequence files  

Microsoft Academic Search

A Windows application for compositional analysis of sequenced genomes (EMBL or GenBank flat files) is available as freeware.\\u000a The application allows the user to quantify word bias using Markov chain analysis and it allows the user to generate sliding\\u000a window data for GC-skew, AT-skew, purine excess, keto excess and discrete word counts. The mathematical routines reside in\\u000a a dynamic link

Anders Fuglsang

2004-01-01

177

MicroGen: a MIAME compliant web system for microarray experiment information and workflow management  

PubMed Central

Background Improvements of bio-nano-technologies and biomolecular techniques have led to increasing production of high-throughput experimental data. Spotted cDNA microarray is one of the most diffuse technologies, used in single research laboratories and in biotechnology service facilities. Although they are routinely performed, spotted microarray experiments are complex procedures entailing several experimental steps and actors with different technical skills and roles. During an experiment, involved actors, who can also be located in a distance, need to access and share specific experiment information according to their roles. Furthermore, complete information describing all experimental steps must be orderly collected to allow subsequent correct interpretation of experimental results. Results We developed MicroGen, a web system for managing information and workflow in the production pipeline of spotted microarray experiments. It is constituted of a core multi-database system able to store all data completely characterizing different spotted microarray experiments according to the Minimum Information About Microarray Experiments (MIAME) standard, and of an intuitive and user-friendly web interface able to support the collaborative work required among multidisciplinary actors and roles involved in spotted microarray experiment production. MicroGen supports six types of user roles: the researcher who designs and requests the experiment, the spotting operator, the hybridisation operator, the image processing operator, the system administrator, and the generic public user who can access the unrestricted part of the system to get information about MicroGen services. Conclusion MicroGen represents a MIAME compliant information system that enables managing workflow and supporting collaborative work in spotted microarray experiment production. PMID:16351755

Burgarella, Sarah; Cattaneo, Dario; Pinciroli, Francesco; Masseroli, Marco

2005-01-01

178

Generalized background error covariance matrix model (GEN_BE v2.0)  

NASA Astrophysics Data System (ADS)

The specification of state background error statistics is a key component of data assimilation since it affects the impact observations will have on the analysis. In the variational data assimilation approach, applied in geophysical sciences, the dimensions of the background error covariance matrix (B) are usually too large to be explicitly determined and B needs to be modeled. Recent efforts to include new variables in the analysis such as cloud parameters and chemical species have required the development of the code to GENerate the Background Errors (GEN_BE) version 2.0 for the Weather Research and Forecasting (WRF) community model. GEN_BE allows for a simpler, flexible, robust, and community-oriented framework that gathers methods used by some meteorological operational centers and researchers. We present the advantages of this new design for the data assimilation community by performing benchmarks of different modeling of B and showing some of the new features in data assimilation test cases. As data assimilation for clouds remains a challenge, we present a multivariate approach that includes hydrometeors in the control variables and new correlated errors. In addition, the GEN_BE v2.0 code is employed to diagnose error parameter statistics for chemical species, which shows that it is a tool flexible enough to implement new control variables. While the generation of the background errors statistics code was first developed for atmospheric research, the new version (GEN_BE v2.0) can be easily applied to other domains of science and chosen to diagnose and model B. Initially developed for variational data assimilation, the model of the B matrix may be useful for variational ensemble hybrid methods as well.

Descombes, G.; Auligné, T.; Vandenberghe, F.; Barker, D. M.; Barré, J.

2015-03-01

179

Structural materials for Gen-IV nuclear reactors: Challenges and opportunities  

Microsoft Academic Search

Generation-IV reactor design concepts envisioned thus far cater toward a common goal of providing safer, longer lasting, proliferation-resistant and economically viable nuclear power plants. The foremost consideration in the successful development and deployment of Gen-IV reactor systems is the performance and reliability issues involving structural materials for both in-core and out-of-core applications. The structural materials need to endure much higher

K. L. Murty; I. Charit

2008-01-01

180

Enhanced and synthetic vision for terminal maneuvering area NextGen operations  

NASA Astrophysics Data System (ADS)

Synthetic Vision Systems and Enhanced Flight Vision System (SVS/EFVS) technologies have the potential to provide additional margins of safety for aircrew performance and enable operational improvements for low visibility operations in the terminal area environment with equivalent efficiency as visual operations. To meet this potential, research is needed for effective technology development and implementation of regulatory and design guidance to support introduction and use of SVS/EFVS advanced cockpit vision technologies in Next Generation Air Transportation System (NextGen) operations. A fixed-base pilot-in-the-loop simulation test was conducted at NASA Langley Research Center that evaluated the use of SVS/EFVS in NextGen low visibility ground (taxi) operations and approach/landing operations. Twelve crews flew approach and landing operations in a simulated NextGen Chicago O'Hare environment. Various scenarios tested the potential for EFVS for operations in visibility as low as 1000 ft runway visibility range (RVR) and SVS to enable lower decision heights (DH) than can currently be flown today. Expanding the EFVS visual segment from DH to the runway in visibilities as low as 1000 RVR appears to be viable as touchdown performance was excellent without any workload penalties noted for the EFVS concept tested. A lower DH to 150 ft and/or possibly reduced visibility minima by virtue of SVS equipage appears to be viable when implemented on a Head-Up Display, but the landing data suggests further study for head-down implementations.

Kramer, Lynda J.; Bailey, Randall E.; Ellis, Kyle K. E.; Norman, R. Michael; Williams, Steven P.; Arthur, Jarvis J., III; Shelton, Kevin J.; Prinzel, Lawrence J., III

2011-06-01

181

Leptojacobus dorci n. gen., n. sp. (Nematoda: Diplogastridae), an Associate of Dorcus Stag Beetles (Coleoptera: Lucanidae).  

PubMed

A new species of diplogastrid nematode, Leptojacobus dorci n. gen., n. sp., was isolated from adults of the stag beetle Dorcus ritsemae (Coleoptera: Lucanidae) that were purchased from a pet shop in Japan. Leptojacobus n. gen. is circumscribed by a very thin, delicate body and by a small stoma with minute armature. A combination of other stomatal characters, namely the division of the cheilostom into adradial plates, the symmetry of the subventral stegostomatal sectors, and the presence of a thin, conical dorsal tooth, further distinguishes Leptojacobus n. gen. from other genera of Diplogastridae. Phylogenetic analysis of nearly full-length SSU rRNA sequences support the new species, together with an isolate identified previously as Koerneria luziae, to be excluded from a clade including all other molecularly characterized diplogastrids with teeth and stomatal dimorphism. Therefore, the new species will be of importance for reconstruction of ancestral character histories in Diplogastridae, a family circumscribed by a suite of feeding-related novelties. PMID:24644371

Kanzaki, Natsumi; Ragsdale, Erik J; Susoy, Vladislav; Sommer, Ralf J

2014-03-01

182

Leptojacobus dorci n. gen., n. sp. (Nematoda: Diplogastridae), an Associate of Dorcus Stag Beetles (Coleoptera: Lucanidae)  

PubMed Central

A new species of diplogastrid nematode, Leptojacobus dorci n. gen., n. sp., was isolated from adults of the stag beetle Dorcus ritsemae (Coleoptera: Lucanidae) that were purchased from a pet shop in Japan. Leptojacobus n. gen. is circumscribed by a very thin, delicate body and by a small stoma with minute armature. A combination of other stomatal characters, namely the division of the cheilostom into adradial plates, the symmetry of the subventral stegostomatal sectors, and the presence of a thin, conical dorsal tooth, further distinguishes Leptojacobus n. gen. from other genera of Diplogastridae. Phylogenetic analysis of nearly full-length SSU rRNA sequences support the new species, together with an isolate identified previously as Koerneria luziae, to be excluded from a clade including all other molecularly characterized diplogastrids with teeth and stomatal dimorphism. Therefore, the new species will be of importance for reconstruction of ancestral character histories in Diplogastridae, a family circumscribed by a suite of feeding-related novelties. PMID:24644371

Kanzaki, Natsumi; Ragsdale, Erik J.; Susoy, Vladislav; Sommer, Ralf J.

2014-01-01

183

Swedish Plectida (Nematoda). Part 6. Neocamacolaimus parasiticus gen. n., sp. n.  

PubMed

A new genus Neocamacolaimus gen. n., with one new species N. parasiticus gen. n., sp. n. is described from the benthic polychaete Sphaerosyllis cf. hystrix collected in the Skagerrak off the west coast of Sweden. Neocamacolaimus gen. n. is placed in the family Camacolaimidae and is particularly characterised by having annulated cuticle with lateral alae; setiform cephalic sensilla located at level with amphids; amphidial fovea ventrally spiral; buccal cavity without armament; pharynx muscular; nerve ring located at base of pharynx; male reproductive system diorchic with outstretched testes; spicules weakly arcuate with straight manubrium; gubernaculum absent; alveolar supplements located in the pharyngeal region; tubular supplements absent; tail conoid; caudal glands and spinneret present. Juveniles of this genus are particularly characterised by their parasitic lifestyle and the following unique morphological features: lips form a dorso-ventrally elongated perioral disc with internal sclerotizations: one midventral and two dorsosublateral (right and left); cephalic sensilla setiform, subventral sensilla are noticeably longer than the subdorsal ones; intestine extends posterior to rectum and anal opening, forming a post-anal pouch. PMID:24989765

Holovachov, Oleksandr; Boström, Sven

2014-01-01

184

Enhanced and Synthetic Vision for Terminal Maneuvering Area NextGen Operations  

NASA Technical Reports Server (NTRS)

Synthetic Vision Systems and Enhanced Flight Vision System (SVS/EFVS) technologies have the potential to provide additional margins of safety for aircrew performance and enable operational improvements for low visibility operations in the terminal area environment with equivalent efficiency as visual operations. To meet this potential, research is needed for effective technology development and implementation of regulatory and design guidance to support introduction and use of SVS/EFVS advanced cockpit vision technologies in Next Generation Air Transportation System (NextGen) operations. A fixed-base pilot-in-the-loop simulation test was conducted at NASA Langley Research Center that evaluated the use of SVS/EFVS in NextGen low visibility ground (taxi) operations and approach/landing operations. Twelve crews flew approach and landing operations in a simulated NextGen Chicago O Hare environment. Various scenarios tested the potential for EFVS for operations in visibility as low as 1000 ft runway visibility range (RVR) and SVS to enable lower decision heights (DH) than can currently be flown today. Expanding the EFVS visual segment from DH to the runway in visibilities as low as 1000 RVR appears to be viable as touchdown performance was excellent without any workload penalties noted for the EFVS concept tested. A lower DH to 150 ft and/or possibly reduced visibility minima by virtue of SVS equipage appears to be viable when implemented on a Head-Up Display, but the landing data suggests further study for head-down implementations.

Kramer, Lynda J.; Bailey, Randall E.; Ellis, Kyle K. E.; Norman, R. Michael; Williams, Steven P.; Arthur, Jarvis J., III; Shelton, Kevin J.; Prinzel, Lawrence J., III

2011-01-01

185

Roseimaritima ulvae gen. nov., sp. nov. and Rubripirellula obstinata gen. nov., sp. nov. two novel planctomycetes isolated from the epiphytic community of macroalgae.  

PubMed

Four isolates, belonging to the deep-branching phylum Planctomycetes, were recovered from the biofilm of two marine macroalgae, Ulva sp. and Laminaria sp., from the Northern coast of Portugal. These strains were light pink- or red-pigmented; the cells were variable in shape and usually organized in rosettes. They had a dimorphic cell cycle with budding reproduction. The organisms were chemoheterotrophic, strictly aerobic and mesophilic. The 16S rRNA gene sequence analysis showed that the strains belong to the family Planctomycetaceae with Rhodopirellula as the closest genus. The isolates form two separate branches (strain LF1(T) forms one branch and the strains UC8(T), UF3 and UF42 form a second branch) clearly separated from Rhodopirellula baltica with 94.2% and 93.8% 16S rRNA gene sequence similarity, respectively. Based on differential characteristics that distinguish the novel genera from R. baltica, such as cell size and shape, ultrastructure, enzymatic activities, substrate utilization pattern, fatty acid composition, phospholipid profiles and phylogeny we propose that the isolates represent two novel genera of the order Planctomycetales, Roseimaritima ulvae gen. nov., sp. nov. (type strain is UC8(T)=DSM 25454(T)=LMG 27778(T)) and Rubripirellula obstinata gen. nov., sp. nov. (type strain is LF1(T)=LMG 27779(T)=CECT 8602(T)). PMID:25487949

Bondoso, Joana; Albuquerque, Luciana; Nobre, M Fernanda; Lobo-da-Cunha, Alexandre; da Costa, Milton S; Lage, Olga Maria

2015-02-01

186

Genome-scale data suggest reclassifications in the Leisingera-Phaeobacter cluster including proposals for Sedimentitalea gen. nov. and Pseudophaeobacter gen. nov.  

PubMed Central

Earlier phylogenetic analyses of the marine Rhodobacteraceae (class Alphaproteobacteria) genera Leisingera and Phaeobacter indicated that neither genus might be monophyletic. We here used phylogenetic reconstruction from genome-scale data, MALDI-TOF mass-spectrometry analysis and a re-assessment of the phenotypic data from the literature to settle this matter, aiming at a reclassification of the two genera. Neither Phaeobacter nor Leisingera formed a clade in any of the phylogenetic analyses conducted. Rather, smaller monophyletic assemblages emerged, which were phenotypically more homogeneous, too. We thus propose the reclassification of Leisingera nanhaiensis as the type species of a new genus as Sedimentitalea nanhaiensis gen. nov., comb. nov., the reclassification of Phaeobacter arcticus and Phaeobacter leonis as Pseudophaeobacter arcticus gen. nov., comb. nov. and Pseudophaeobacter leonis comb. nov., and the reclassification of Phaeobacter aquaemixtae, Phaeobacter caeruleus, and Phaeobacter daeponensis as Leisingera aquaemixtae comb. nov., Leisingera caerulea comb. nov., and Leisingera daeponensis comb. nov. The genera Phaeobacter and Leisingera are accordingly emended. PMID:25157246

Breider, Sven; Scheuner, Carmen; Schumann, Peter; Fiebig, Anne; Petersen, Jörn; Pradella, Silke; Klenk, Hans-Peter; Brinkhoff, Thorsten; Göker, Markus

2014-01-01

187

FutureGen: Stepping-Stone to Sustainable Fossil-Fuel Power Generation  

SciTech Connect

This presentation will highlight the U.S. Department of Energy's FutureGen Initiative. The nearly $1 billion government-industry project is a stepping-stone toward future coal-fired power plants that will produce hydrogen and electricity with zero-emissions, including carbon dioxide. The 275-megawatt FutureGen plant will initiate operations around 2012 and employ advanced coal gasification technology integrated with combined cycle electricity generation, hydrogen production, and carbon capture and sequestration. The initiative is a response to a presidential directive to develop a hydrogen economy by drawing upon the best scientific research to address the issue of global climate change. The FutureGen plant will be based on cutting-edge power generation technology as well as advanced carbon capture and sequestration systems. The centerpiece of the project will be coal gasification technology that can eliminate common air pollutants such as sulfur dioxide and nitrogen oxides and convert them to useable by-products. Gasification will convert coal into a highly enriched hydrogen gas, which can be burned much more cleanly than directly burning the coal itself. Alternatively, the hydrogen can be used in a fuel cell to produce ultra-clean electricity, or fed to a refinery to help upgrade petroleum products. Carbon sequestration will also be a key feature that will set the Futuregen plant apart from other electric power plant projects. The initial goal will be to capture 90 percent of the plant's carbon dioxide, but capture of nearly 100 percent may be possible with advanced technologies. Once captured, the carbon dioxide will be injected as a compressed fluid deep underground, perhaps into saline reservoirs. It could even be injected into oil or gas reservoirs, or into unmineable coal seams, to enhance petroleum or coalbed methane recovery. The ultimate goal for the FutureGen plant is to show how new technology can eliminate environmental concerns over the future use of coal--the most abundant fossil fuel in the United States with supplies projected to last 250 years. FutureGen's co-production of power and hydrogen will also serve as a stepping-stone to an environmentally sustainable energy future.

Zitney, S.E.

2006-11-01

188

Roles of SLX1-SLX4, MUS81-EME1, and GEN1 in avoiding genome instability and mitotic catastrophe.  

PubMed

The resolution of recombination intermediates containing Holliday junctions (HJs) is critical for genome maintenance and proper chromosome segregation. Three pathways for HJ processing exist in human cells and involve the following enzymes/complexes: BLM-TopoIII?-RMI1-RMI2 (BTR complex), SLX1-SLX4-MUS81-EME1 (SLX-MUS complex), and GEN1. Cycling cells preferentially use the BTR complex for the removal of double HJs in S phase, with SLX-MUS and GEN1 acting at temporally distinct phases of the cell cycle. Cells lacking SLX-MUS and GEN1 exhibit chromosome missegregation, micronucleus formation, and elevated levels of 53BP1-positive G1 nuclear bodies, suggesting that defects in chromosome segregation lead to the transmission of extensive DNA damage to daughter cells. In addition, however, we found that the effects of SLX4, MUS81, and GEN1 depletion extend beyond mitosis, since genome instability is observed throughout all phases of the cell cycle. This is exemplified in the form of impaired replication fork movement and S-phase progression, endogenous checkpoint activation, chromosome segmentation, and multinucleation. In contrast to SLX4, SLX1, the nuclease subunit of the SLX1-SLX4 structure-selective nuclease, plays no role in the replication-related phenotypes associated with SLX4/MUS81 and GEN1 depletion. These observations demonstrate that the SLX1-SLX4 nuclease and the SLX4 scaffold play divergent roles in the maintenance of genome integrity in human cells. PMID:24831703

Sarbajna, Shriparna; Davies, Derek; West, Stephen C

2014-05-15

189

Data-Link and Surface Map Traffic Intent Displays for NextGen 4DT and Equivalent Visual Surface Operations  

NASA Technical Reports Server (NTRS)

By 2025, U.S. air traffic is predicted to increase 3-fold and may strain the current air traffic management system, which may not be able to accommodate this growth. In response to this challenge, a consortium of industry, academia and government agencies have proposed a revolutionary new concept for U.S. aviation operations, termed the Next Generation Air Transportation System or "NextGen". Many key capabilities are being identified to enable NextGen, including the concept of "net-centric" operations whereby each aircraft and air services provider shares information to allow real-time adaptability to ever-changing factors such as weather, traffic, flight trajectories, and security. Data-link is likely to be the primary source of communication in NextGen. Because NextGen represents a radically different approach to air traffic management and requires a dramatic shift in the tasks, roles, and responsibilities for the flight deck, there are numerous research issues and challenges that must be overcome to ensure a safe, sustainable air transportation system. Flight deck display and crew-vehicle interaction concepts are being developed that proactively investigate and overcome potential technology and safety barriers that might otherwise constrain the full realization of NextGen.

Shelton, Kevin J.; Prinzel, Lawrence J., III; Arthur, Jarvis J., III; Jones, Deise R.; Allamandola, Angela S.; Bailey, Randall E.

2009-01-01

190

Hungatella effluvii gen. nov., sp. nov., an obligately anaerobic bacterium isolated from an effluent treatment plant, and reclassification of Clostridium hathewayi as Hungatella hathewayi gen. nov., comb. nov.  

PubMed

A Gram-stain-positive, rod-shaped, spore-forming and strictly anaerobic bacterium, designated UB-B.2(T), was isolated from an industrial effluent anaerobic digester sample. It grew optimally at 30 °C and pH 7.0. Comparative analysis of the 16S rRNA gene sequence confirmed that strain UB-B.2(T) was closely related to Clostridium hathewayi DSM 13479(T) (97.84% similarity), a member of rRNA gene cluster XIVa of the genus Clostridium, and formed a coherent cluster with other related members of the Blautia (Clostridium) coccoides rRNA group in phylogenetic analyses. The end products of glucose fermentation by strain UB-B.2(T) were acetate and propionate. The G+C content of the DNA was 51.4 mol%. Although strain UB-B.2(T) showed 97.8% 16S rRNA gene sequence identity to the type strain of C. hathewayi, it exhibited only 38.4% relatedness at the whole-genome level. It also showed differences from its closest phylogenetic relative, C. hathewayi DSM 13479(T), in phenotypic characteristics such as hydrolysis of aesculin, starch and urea and fermentation end products. Both strains showed phenotypic differences from the members of rRNA gene cluster XIVa of the genus Clostridium. Based on these differences, C. hathewayi DSM 13479(T) and strain UB-B.2(T) were identified as representatives of a new genus of the family Clostridiaceae. Thus, we propose the reclassification of Clostridium hathewayi as Hungatella hathewayi gen. nov., comb. nov., the type species of the new genus (type strain DSM 13479(T)?=?CCUG 43506(T)?=?MTCC 10951(T)). Strain UB-B.2(T) (?=?MTCC 11101(T)?=?DSM 24995(T)) is assigned to the novel species Hungatella effluvii gen. nov., sp. nov as the type strain. PMID:24186873

Kaur, Sukhpreet; Yawar, Mir; Kumar, P Anil; Suresh, K

2014-03-01

191

G:\\FPDC\\Contracts Unit\\GCCM\\Appendix D GCCM_Gen_Conditions_Cost_Worksheet.doc GUARANTEED MAXIMUM COST FOR REIMBURSABLE EXPENSES  

E-print Network

G:\\FPDC\\Contracts Unit\\GCCM\\Appendix D GCCM_Gen_Conditions_Cost_Worksheet.doc GUARANTEED MAXIMUM Secretary 10 Clerk 11 Other 12 Other 13 Other Subtotal #12;G:\\FPDC\\Contracts Unit\\GCCM\\Appendix D GCCM_Gen_Conditions Subtotal 300 Field Office Rental Quantity Unit Unit Price GMC 1 Rent 2 Set-Up and Dismantle 3 Power 4 Water

Dyer, Bill

192

Next Gen NEAR: Near Earth Asteroid Human Robotic Precursor Mission Concept  

NASA Technical Reports Server (NTRS)

Asteroids have long held the attention of the planetary science community. In particular, asteroids that evolve into orbits near that of Earth, called near-Earth objects (NEO), are of high interest as potential targets for exploration due to the relative ease (in terms of delta V) to reach them. NASA's Flexible Path calls for missions and experiments to be conducted as intermediate steps towards the eventual goal of human exploration of Mars; piloted missions to NEOs are such example. A human NEO mission is a valuable exploratory step beyond the Earth-Moon system enhancing capabilities that surpass our current experience, while also developing infrastructure for future mars exploration capabilities. To prepare for a human rendezvous with an NEO, NASA is interested in pursuing a responsible program of robotic NEO precursor missions. Next Gen NEAR is such a mission, building on the NEAR Shoemaker mission experience at the JHU/APL Space Department, to provide an affordable, low risk solution with quick data return. Next Gen NEAR proposes to make measurements needed for human exploration to asteroids: to demonstrate proximity operations, to quantify hazards for human exploration and to characterize an environment at a near-Earth asteroid representative of those that may be future human destinations. The Johns Hopkins University Applied Physics Laboratory has demonstrated exploration-driven mission feasibility by developing a versatile spacecraft design concept using conventional technologies that satisfies a set of science, exploration and mission objectives defined by a concept development team in the summer of 2010. We will describe the mission concept and spacecraft architecture in detail. Configuration options were compared with the mission goals and objectives in order to select the spacecraft design concept that provides the lowest cost, lowest implementation risk, simplest operation and the most benefit for the mission implementation. The Next Gen NEAR spacecraft was designed to support rendezvous with a range of candidate asteroid targets and could easily be launched with one of several NASA launch vehicles. The Falcon 9 launch vehicle supports a Next Gen NEAR launch to target many near-Earth asteroids under consideration that could be reached with a C3 of 18 km2/sec2 or less, and the Atlas V-401 provides added capability supporting launch to NEAs that require more lift capacity while at the same time providing such excess lift capability that another payload of opportunity could be launch in conjunction with Next Gen NEAR. Next Gen NEAR will measure and interact with the target surface in ways never undertaken at an asteroid, and will prepare for first human precursor mission by demonstrating exploration science operations at an accessible NEO. This flexible mission and spacecraft design concept supports target selection based on upcoming Earth-based observations and also provides opportunities for co-manifest & international partnerships. JHU/APL has demonstrated low cost, low risk, high impact missions and this mission will help to prepare NASA for human NEO exploration by combining the best of NASA s human and robotic exploration capabilities.

Rivkin, Andrew S.; Kirby, Karen; Cheng, Andrew F.; Gold, Robert; Kelly, Daniel; Reed, Cheryl; Abell, Paul; Garvin, James; Landis, Rob

2012-01-01

193

Considerations of Alloy 617 Application in the Gen IV Nuclear Reactor Systems - Part I: Mechanical Property Challenges  

SciTech Connect

Alloy 617 is currently considered as a leading candidate material for high temperature components in the Gen IV Nuclear Reactor Systems. Because of the unprecedented severe working conditions beyond its commercial service experience required by the Gen IV systems, the alloy faces various challenges in both mechanical and metallurgical properties. This paper, as Part I of the discussion, is focused on the challenges and issues in the mechanical properties of Alloy 617 for the intended nuclear application. Considerations are given in details in its mechanical property data scatter, low creep strength in the desired high temperature range, lack of longterm creep curves, high loading rate dependency, and preponderant tertiary creep. Some research and development activities are suggested with discussions on their viability to satisfy the Gen IV Nuclear Reactor System needs in near future and in the long run.

Ren, Weiju [ORNL

2010-01-01

194

Considerations of Alloy 617 Application in the Gen IV Nuclear Reactor Systems - Part II: Metallurgical Property Challenges  

SciTech Connect

Alloy 617 is currently considered as a leading candidate material for high temperature components in the Gen IV Nuclear Reactor Systems. Because of the unprecedented severe working conditions beyond its commercial service experience required by the Gen IV systems, the alloy faces various challenges in both mechanical and metallurgical properties. Following a previous paper discussing the mechanical property challenges, this paper is focused on the challenges and issues in metallurgical properties of the alloy for the intended nuclear application. Considerations are given in details about its metallurgical stability and aging evolution, aging effects on mechanical properties, potential Co hazard, and internal oxidation. Some research and development activities are suggested with discussions on viability to satisfy the Gen IV Nuclear Reactor System needs.

Ren, Weiju [ORNL

2010-01-01

195

Generalized Background Error covariance matrix model (GEN_BE v2.0)  

NASA Astrophysics Data System (ADS)

The specification of state background error statistics is a key component of data assimilation since it affects the impact observations will have on the analysis. In the variational data assimilation approach, applied in geophysical sciences, the dimensions of the background error covariance matrix (B) are usually too large to be explicitly determined and B needs to be modeled. Recent efforts to include new variables in the analysis such as cloud parameters and chemical species have required the development of the code to GENerate the Background Errors (GEN_BE) version 2.0 for the Weather Research and Forecasting (WRF) community model to allow for a simpler, flexible, robust, and community-oriented framework that gathers methods used by meteorological operational centers and researchers. We present the advantages of this new design for the data assimilation community by performing benchmarks and showing some of the new features on data assimilation test cases. As data assimilation for clouds remains a challenge, we present a multivariate approach that includes hydrometeors in the control variables and new correlated errors. In addition, the GEN_BE v2.0 code is employed to diagnose error parameter statistics for chemical species, which shows that it is a tool flexible enough to involve new control variables. While the generation of the background errors statistics code has been first developed for atmospheric research, the new version (GEN_BE v2.0) can be easily extended to other domains of science and be chosen as a testbed for diagnostic and new modeling of B. Initially developed for variational data assimilation, the model of the B matrix may be useful for variational ensemble hybrid methods as well.

Descombes, G.; Auligné, T.; Vandenberghe, F.; Barker, D. M.

2014-07-01

196

Enhanced Flight Vision Systems and Synthetic Vision Systems for NextGen Approach and Landing Operations  

NASA Technical Reports Server (NTRS)

Synthetic Vision Systems and Enhanced Flight Vision System (SVS/EFVS) technologies have the potential to provide additional margins of safety for aircrew performance and enable operational improvements for low visibility operations in the terminal area environment with equivalent efficiency as visual operations. To meet this potential, research is needed for effective technology development and implementation of regulatory standards and design guidance to support introduction and use of SVS/EFVS advanced cockpit vision technologies in Next Generation Air Transportation System (NextGen) operations. A fixed-base pilot-in-the-loop simulation test was conducted at NASA Langley Research Center that evaluated the use of SVS/EFVS in NextGen low visibility approach and landing operations. Twelve crews flew approach and landing operations in a simulated NextGen Chicago O'Hare environment. Various scenarios tested the potential for using EFVS to conduct approach, landing, and roll-out operations in visibility as low as 1000 feet runway visual range (RVR). Also, SVS was tested to evaluate the potential for lowering decision heights (DH) on certain instrument approach procedures below what can be flown today. Expanding the portion of the visual segment in which EFVS can be used in lieu of natural vision from 100 feet above the touchdown zone elevation to touchdown and rollout in visibilities as low as 1000 feet RVR appears to be viable as touchdown performance was acceptable without any apparent workload penalties. A lower DH of 150 feet and/or possibly reduced visibility minima using SVS appears to be viable when implemented on a Head-Up Display, but the landing data suggests further study for head-down implementations.

Kramer, Lynda J.; Bailey, Randall E.; Ellis, Kyle K. E.; Williams, Steven P.; Arthur, Jarvis J., III; Prinzel, Lawrence J., III; Shelton, Kevin J.

2013-01-01

197

Obesoconnus gen. n., a new bizarre Neotropical genus of Cyrtoscydmini (Coleoptera: Staphylinidae: Scydmaeninae).  

PubMed

A new Neotropical genus of Cyrtoscydmini, Obesoconnus gen. n., is described to accommodate two new species: O. guyanensis sp. n. (the type species of Obesoconnus) from French Guyana and O. mexicanus sp. n. from Mexico. The morphology of the new taxon is described and illustrated in detail, and its possible affinities to other Cyrtoscydmini are discussed. Despite external dissimilarities, the aedeagus of Obesoconnus highly resembles copulatory organs of Alloraphes and Parastenichnaphes; all these genera share the basal pumping apparatus and apically located ostium with asymmetrical apical projections. PMID:24870336

Ja?oszy?ski, Pawe?

2014-01-01

198

Gen IV Materials Handbook Functionalities and Operation (4A) Handbook Version 4.0  

SciTech Connect

This document is prepared for navigation and operation of the Gen IV Materials Handbook, with architecture description and new user access initiation instructions. Development rationale and history of the Handbook is summarized. The major development aspects, architecture, and design principles of the Handbook are briefly introduced to provide an overview of its past evolution and future prospects. Detailed instructions are given with examples for navigating the constructed Handbook components and using the main functionalities. Procedures are provided in a step-by-step fashion for Data Upload Managers to upload reports and data files, as well as for new users to initiate Handbook access.

Ren, Weiju [ORNL

2013-09-01

199

Kennedy Space Center Next Gen Site: Routine, Affordable Access to Space  

NSDL National Science Digital Library

The Next Gen Site, operated by the National Aeronautics and Space Administration at Kennedy Space Center, is investigating ways of making space travel more routine and affordable. The site offers many reports on the Vision Spaceport Partnership, the Highly Reusable Space Transportation Project, Space Solar Power, and Space Transportation System Affordability. A map of Cape Canaveral highlights each launch pad, and you can see information on each one by clicking on it. Related Info Including X-Vehicles is a section with background on several experimental projects and has links to sites that examine different aspects of design and planning for easier access to space.

200

Autosomal SNP typing of forensic samples with the GenPlex™ HID System: results of a collaborative study.  

PubMed

The GenPlex™ HID System (Applied Biosystems - AB) offers typing of 48 of the 52 SNPforID SNPs and amelogenin. Previous studies have shown a high reproducibility of the GenPlex™ HID System using 250-500pg DNA of good quality. An international exercise was performed by 14 laboratories (9 in Europe and 5 in the US) in order to test the robustness and reliability of the GenPlex™ HID System on forensic samples. Three samples with partly degraded DNA and 10 samples with low amounts of DNA were analyzed in duplicates using various amounts of DNA. In order to compare the performance of the GenPlex™ HID System with the most commonly used STR kits, 500pg of partly degraded DNA from three samples was typed by the laboratories using one or more STR kits. The median SNP typing success rate was 92.3% with 500pg of partly degraded DNA. Three of the fourteen laboratories counted for more than two thirds of the locus dropouts. The median percentage of discrepant results was 0.2% with 500pg degraded DNA. An increasing percentage of locus dropouts and discrepant results were observed when lower amounts of DNA were used. Different success rates were observed for the various SNPs. The rs763869 SNP was the least successful. With the exception of the MiniFiler™ kit (AB), GenPlex™ HID performed better than five other tested STR kits. When partly degraded DNA was analyzed, GenPlex™ HID showed a very low mean mach probability, while all STR kits except MiniFiler™ had very limited discriminatory power. PMID:20650697

Tomas, C; Axler-DiPerte, G; Budimlija, Z M; Børsting, C; Coble, M D; Decker, A E; Eisenberg, A; Fang, R; Fondevila, M; Fredslund, S Frisk; Gonzalez, S; Hansen, A J; Hoff-Olsen, P; Haas, C; Kohler, P; Kriegel, A K; Lindblom, B; Manohar, F; Maroñas, O; Mogensen, H S; Neureuther, K; Nilsson, H; Scheible, M K; Schneider, P M; Sonntag, M L; Stangegaard, M; Syndercombe-Court, D; Thacker, C R; Vallone, P M; Westen, A A; Morling, N

2011-11-01

201

Brockphasma spinifemoralis gen. et spec. nov.: a new phasmid genus and new species of Neohiraseini (Phasmida: Necrosciinae) from Vietnam.  

PubMed

A new genus of stick insects, Brockphasma Ho gen. nov., with the type-species, Brockphasma spinifemoralis Ho, Liu, Bresseel & Constant spec. nov., is described and illustrated from Vietnam. Both sexes, the egg and the first instar nymph are described and figured. Data on the habitat and natural foodplants are provided. Brockphasma Ho gen. nov. is differentiated from other genera in Neohiraseini by spinose occiput, anterior region of mesonotum with a spinose hump and spinose anterodorsal and posterodorsal carinae of femora. A key to the genera of Neohiraseini from Vietnam is given. PMID:24990046

Ho, George Wai-Chun; Liu, Xing-Yue; Bresseel, Joachim; Constant, Jerome

2014-01-01

202

Flight Deck Technologies to Enable NextGen Low Visibility Surface Operations  

NASA Technical Reports Server (NTRS)

Many key capabilities are being identified to enable Next Generation Air Transportation System (NextGen), including the concept of Equivalent Visual Operations (EVO) . replicating the capacity and safety of today.s visual flight rules (VFR) in all-weather conditions. NASA is striving to develop the technologies and knowledge to enable EVO and to extend EVO towards a Better-Than-Visual operational concept. This operational concept envisions an .equivalent visual. paradigm where an electronic means provides sufficient visual references of the external world and other required flight references on flight deck displays that enable Visual Flight Rules (VFR)-like operational tempos while maintaining and improving safety of VFR while using VFR-like procedures in all-weather conditions. The Langley Research Center (LaRC) has recently completed preliminary research on flight deck technologies for low visibility surface operations. The work assessed the potential of enhanced vision and airport moving map displays to achieve equivalent levels of safety and performance to existing low visibility operational requirements. The work has the potential to better enable NextGen by perhaps providing an operational credit for conducting safe low visibility surface operations by use of the flight deck technologies.

Prinzel, Lawrence (Lance) J., III; Arthur, Jarvis (Trey) J.; Kramer, Lynda J.; Norman, Robert M.; Bailey, Randall E.; Jones, Denise R.; Karwac, Jerry R., Jr.; Shelton, Kevin J.; Ellis, Kyle K. E.

2013-01-01

203

PubAngioGen: a database and knowledge for angiogenesis and related diseases.  

PubMed

Angiogenesis is the process of generating new blood vessels based on existing ones, which is involved in many diseases including cancers, cardiovascular diseases and diabetes mellitus. Recently, great efforts have been made to explore the mechanisms of angiogenesis in various diseases and many angiogenic factors have been discovered as therapeutic targets in anti- or pro-angiogenic drug development. However, the resulted information is sparsely distributed and no systematical summarization has been made. In order to integrate these related results and facilitate the researches for the community, we conducted manual text-mining from published literature and built a database named as PubAngioGen (http://www.megabionet.org/aspd/). Our online application displays a comprehensive network for exploring the connection between angiogenesis and diseases at multilevels including protein-protein interaction, drug-target, disease-gene and signaling pathways among various cells and animal models recorded through text-mining. To enlarge the scope of the PubAngioGen application, our database also links to other common resources including STRING, DrugBank and OMIM databases, which will facilitate understanding the underlying molecular mechanisms of angiogenesis and drug development in clinical therapy. PMID:25392416

Li, Peng; Liu, Yongrui; Wang, Huan; He, Yuan; Wang, Xue; He, Yundong; Lv, Fang; Chen, Huaqing; Pang, Xiufeng; Liu, Mingyao; Shi, Tieliu; Yi, Zhengfang

2015-01-01

204

Advanced Technology Development Program for Lithium-Ion Batteries: Gen 2 Performance Evaluation Final Report  

SciTech Connect

The Advanced Technology Development Program has completed performance testing of the second generation of lithium-ion cells (i.e., Gen 2 cells). The 18650-size Gen 2 cells, with a baseline and variant chemistry, were distributed over a matrix consisting of three states-of-charge (SOCs) (60, 80, and 100% SOC), four temperatures (25, 35, 45, and 55°C), and three life tests (calendar-, cycle-, and accelerated-life). The calendar- and accelerated-life cells were clamped at an open-circuit voltage corresponding to the designated SOC and were subjected to a once-per-day pulse profile. The cycle-life cells were continuously pulsed using a profile that was centered around 60% SOC. Life testing was interrupted every four weeks for reference performance tests (RPTs), which were used to quantify changes in cell degradation as a function of aging. The RPTs generally consisted of C1/1 and C1/25 static capacity tests, a low-current hybrid pulse power characterization test, and electrochemical impedance spectroscopy. The rate of cell degradation generally increased with increasing test temperature, and SOC. It was also usually slowest for the calendar-life cells and fastest for the accelerated-life cells. Detailed capacity-, power-, and impedance-based performance results are reported.

Jon P. Christophersen; Ira Bloom; Edward V. Thomas; Kevin L. Gering; Gary L. Henriksen; Vincent S. Battaglia; David Howell

2006-07-01

205

PubAngioGen: a database and knowledge for angiogenesis and related diseases  

PubMed Central

Angiogenesis is the process of generating new blood vessels based on existing ones, which is involved in many diseases including cancers, cardiovascular diseases and diabetes mellitus. Recently, great efforts have been made to explore the mechanisms of angiogenesis in various diseases and many angiogenic factors have been discovered as therapeutic targets in anti- or pro-angiogenic drug development. However, the resulted information is sparsely distributed and no systematical summarization has been made. In order to integrate these related results and facilitate the researches for the community, we conducted manual text-mining from published literature and built a database named as PubAngioGen (http://www.megabionet.org/aspd/). Our online application displays a comprehensive network for exploring the connection between angiogenesis and diseases at multilevels including protein–protein interaction, drug-target, disease-gene and signaling pathways among various cells and animal models recorded through text-mining. To enlarge the scope of the PubAngioGen application, our database also links to other common resources including STRING, DrugBank and OMIM databases, which will facilitate understanding the underlying molecular mechanisms of angiogenesis and drug development in clinical therapy. PMID:25392416

Li, Peng; Liu, Yongrui; Wang, Huan; He, Yuan; Wang, Xue; He, Yundong; Lv, Fang; Chen, Huaqing; Pang, Xiufeng; Liu, Mingyao; Shi, Tieliu; Yi, Zhengfang

2015-01-01

206

Comparison of GenFlex Tag array and Pyrosequencing in SNP genotyping.  

PubMed

With the completion of the Human Genome Project, over 2 million sequence-verified single nucleotide polymorphisms (SNPs) have been deposited in public databases. The challenge has shifted from SNP identification to high-throughput SNP genotyping. Although this has had little impact on molecular diagnostics, it provides the potential for future molecular diagnostics of complex traits to include SNP profiling. Accordingly, efficient, accurate, and flexible SNP genotyping are needed. In addition, the drive for low cost has pushed genotyping reactions toward multiplexing capability. We compared two SNP genotyping techniques: Affymetrix GenFlex Tag array and Pyrosequencing. The reference method was a well-established, solid-phase, single nucleotide extension reaction technique based on tritium detection. Fourteen SNPs were selected from the fine mapping project of a multiple sclerosis locus on chromosome 17q. Using all three techniques and the reference method, the SNPs were analyzed in 96 related individuals. Without extensive optimization, we successfully genotyped 11 of 14 SNPs with both GenFlex and Pyrosequencing. Our study suggests that the Pyrosequencing technique provides higher accuracy between the two systems which is most likely due to the single-stranded template in the extension reaction. Thus, Pyrosequencing has potential for diagnostic applications. Pyrosequencing, however, is not optimal for large SNP profiling analyses wherein multiplexing potential is an advantage. PMID:14573784

Chen, Daniel C; Saarela, Janna; Nuotio, Ilpo; Jokiaho, Anne; Peltonen, Leena; Palotie, Aarno

2003-11-01

207

The FunGenES Database: A Genomics Resource for Mouse Embryonic Stem Cell Differentiation  

PubMed Central

Embryonic stem (ES) cells have high self-renewal capacity and the potential to differentiate into a large variety of cell types. To investigate gene networks operating in pluripotent ES cells and their derivatives, the “Functional Genomics in Embryonic Stem Cells” consortium (FunGenES) has analyzed the transcriptome of mouse ES cells in eleven diverse settings representing sixty-seven experimental conditions. To better illustrate gene expression profiles in mouse ES cells, we have organized the results in an interactive database with a number of features and tools. Specifically, we have generated clusters of transcripts that behave the same way under the entire spectrum of the sixty-seven experimental conditions; we have assembled genes in groups according to their time of expression during successive days of ES cell differentiation; we have included expression profiles of specific gene classes such as transcription regulatory factors and Expressed Sequence Tags; transcripts have been arranged in “Expression Waves” and juxtaposed to genes with opposite or complementary expression patterns; we have designed search engines to display the expression profile of any transcript during ES cell differentiation; gene expression data have been organized in animated graphs of KEGG signaling and metabolic pathways; and finally, we have incorporated advanced functional annotations for individual genes or gene clusters of interest and links to microarray and genomic resources. The FunGenES database provides a comprehensive resource for studies into the biology of ES cells. PMID:19727443

Adler, Priit; Aksoy, Irène; Anastassiadis, Konstantinos; Bader, Michael; Billon, Nathalie; Boeuf, Hélène; Bourillot, Pierre-Yves; Buchholz, Frank; Dani, Christian; Doss, Michael Xavier; Forrester, Lesley; Gitton, Murielle; Henrique, Domingos; Hescheler, Jürgen; Himmelbauer, Heinz; Hübner, Norbert; Karantzali, Efthimia; Kretsovali, Androniki; Lubitz, Sandra; Pradier, Laurent; Rai, Meena; Reimand, Jüri; Rolletschek, Alexandra; Sachinidis, Agapios; Savatier, Pierre; Stewart, Francis; Storm, Mike P.; Trouillas, Marina; Vilo, Jaak; Welham, Melanie J.; Winkler, Johannes; Wobus, Anna M.; Hatzopoulos, Antonis K.

2009-01-01

208

Array-CGH profiling We used human Integrachip V2 to establish genomic profiles (IntegraGen SA, Evry.  

E-print Network

Array-CGH profiling We used human Integrachip V2 to establish genomic profiles (IntegraGen SA, Evry provides preblocked slides. Genomic DNA (300 ng) was labeled by random-priming in a 45µl reaction of the labeled DNA is checked using a spectrophotometer and incorporation of dyes is calculated. A mix of 700

Paris-Sud XI, Université de

209

Arch Gen Psychiatry . Author manuscript Impact of smoking on cognitive decline in early old age: the Whitehall II  

E-print Network

Arch Gen Psychiatry . Author manuscript Page /1 12 Impact of smoking on cognitive decline in early lifespan of smokers. Objective To examine the association between smoking history and cognitive decline the entire study period. Linear mixed models were used to assess the association between smoking history

210

NEW at Northern Illinois University: A Unique Gen-Ed Interdisciplinary Course for Everybody MEE 101 Energy and the Environment  

E-print Network

NEW at Northern Illinois University: A Unique Gen-Ed Interdisciplinary Course for Everybody MEE 101 of living and the impact resulting from use of energy to the environmental pollution (quantitative skills conservation, and prevention of pollution to the environment (synthesis of knowledge from various disciplines

Kostic, Milivoje M.

211

YouGenMap: a web platform for dynamic multi-comparative mapping and visualization of genetic maps.  

PubMed

Comparative genetic maps are used in examination of genome organization, detection of conserved gene order, and exploration of marker order variations. YouGenMap is an open-source web tool that offers dynamic comparative mapping capability of users' own genetic mapping between 2 or more map sets. Users' genetic map data and optional gene annotations are uploaded, either publically or privately, as long as they follow our template which is available in several standard file formats. Data is parsed and loaded into MySQL relational database to be displayed and compared against users' genetic maps or other public data available on YouGenMap. With the highly interactive GUIs, all public data on YouGenMap are maps available for visualization, comparison, search, filtration and download. YouGenMap web tool is available on the website (http://conifergdb.miamioh.edu/yougenmap) with the source-code repository at (http://sourceforge.net/projects/yougenmap/?source=directory). PMID:25009553

Batesole, Keith; Wimalanathan, Kokulapalan; Liu, Lin; Zhang, Fan; Echt, Craig S; Liang, Chun

2014-01-01

212

YouGenMap: a web platform for dynamic multi-comparative mapping and visualization of genetic maps  

PubMed Central

Comparative genetic maps are used in examination of genome organization, detection of conserved gene order, and exploration of marker order variations. YouGenMap is an open-source web tool that offers dynamic comparative mapping capability of users' own genetic mapping between 2 or more map sets. Users' genetic map data and optional gene annotations are uploaded, either publically or privately, as long as they follow our template which is available in several standard file formats. Data is parsed and loaded into MySQL relational database to be displayed and compared against users' genetic maps or other public data available on YouGenMap. With the highly interactive GUIs, all public data on YouGenMap are maps available for visualization, comparison, search, filtration and download. YouGenMap web tool is available on the website (http://conifergdb.miamioh.edu/yougenmap) with the source-code repository at (http://sourceforge.net/projects/yougenmap/?source=directory). PMID:25009553

Batesole, Keith; Wimalanathan, Kokulapalan; Liu, Lin; Zhang, Fan; Echt, Craig S.; Liang, Chun

2014-01-01

213

USDA Forest Service Gen. Tech. Rep. PSW-GTR-203. 2006. 121 Free Selection: A Silvicultural Option1  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-GTR-203. 2006. 121 Free Selection: A Silvicultural Option1 set of procedures well suited for the timely and efficient production of timber crops but too often is paramount. It is well suited for restoring the old- growth character of forests as well as reducing the risk

Standiford, Richard B.

214

Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium mediterraneum, and Rhizobium to Mesorhizobium gen. nov. Rhizobium ciceri, tianshanense  

Microsoft Academic Search

Reasons are advanced for removal of Rhizobium ciceri, Rhizobium huakuii, Rhizobium loti, Rhizobium medi- terraneum, and Rhizobium tianshanense from the genus Rhizobium and for establishment of Mesorhizobium gen. nov. for these species. A description of the genus Mesorhizobium and amended descriptions of Mesorhizobium ciceri, Mesorhizobium huakuii, Mesorhizobium loti, Mesorhizobium mediterraneum, and Mezorhizobium tian- shanense are provided. In a review of

B. D. W. JARVIS; P. VAN BERKUM; W. X. CHEN; S. M. NOUR; M. P. FERNANDEZ; J. C. CLEYET-MAREL; M. GILLIS

215

Description of Bryantinus gen. n. from Sarawak, and New Distributional Records for Cerochusa cilioceps in Thailand (Coleoptera: Staphylinidae: Pselaphinae)  

PubMed Central

A new genus and species of the subtribe Batrisina from western Sarawak, Bryantinus matangus gen. et sp. n., is described, illustrated, and compared with related taxa. In addition, examination of a small series of batrisine material from Thailand revealed a new country record for Cerochusa cilioceps Yin & Nomura, which was previously known only from the island of Hainan in southern China. PMID:25409318

Yin, Zi-Wei; Li, Li-Zhen

2014-01-01

216

A new gastropod mollusc, Antarctissitys austrodema gen. et sp. nov., from the upper Maastrichtian of Antarctica and its evolutionary significance  

Microsoft Academic Search

The Maastrichtian López de Bertodano Formation of Seymour Island, Antarctic Peninsula, hosts the most diverse assemblage of Late Cretaceous molluscs from the continent. Described here is a new genus and species of gastropod, Antarctissitys austrodema gen. et sp. nov., from the upper units of the formation, which are inferred to represent a mid-shelf clastic environment. Antarctissitys austrodema is assignable to

Jeffrey D. Stilwell; William J. Zinsmeister

2003-01-01

217

33USDA Forest Service Gen. Tech. Rep. PSW-GTR-158. 1995. Institutionalizing Fire Safety in Making Land Use and  

E-print Network

Department, P.O.Box 471, Boulder, CO 80306, and Wildland Fire Mitigation Coordinator, City of Boulder Fire the risks of these fire hazards. The Wildfire Hazard Identification and Mitigation System (WHIMS) is used33USDA Forest Service Gen. Tech. Rep. PSW-GTR-158. 1995. Institutionalizing Fire Safety in Making

Standiford, Richard B.

218

45USDA Forest Service Gen. Tech. Rep. PSW-GTR-158. 1995. Legal Barriers to Fuel Management1  

E-print Network

emergencies. Some provisions of the law have led to questions regarding its effectiveness in mitigating fire45USDA Forest Service Gen. Tech. Rep. PSW-GTR-158. 1995. Legal Barriers to Fuel Management1 Anita E. Ruud2 The law is a strict master regarding any kind of deliberately set fires. The value of natural

Standiford, Richard B.

219

USDA Forest Service Gen. Tech. Rep. PSW-GTR-181. 2002. 65 Influence of Bank Afforestation and Snag  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-GTR-181. 2002. 65 Influence of Bank Afforestation and Snag to test the impact of bank afforestation and snag fall direction on delivery trends. Combining all cumulative LWD recruitment across bank afforestation levels averaged 77.1 cubic meters per 100 meter reach

Standiford, Richard B.

220

77 FR 52352 - Notice of Intent To Prepare an Environmental Impact Statement for the Rio Mesa Gen-Tie Project...  

Federal Register 2010, 2011, 2012, 2013, 2014

In compliance with the National Environmental Policy Act of 1969, as amended, (NEPA) and the Federal Land Policy and Management Act of 1976, as amended (FLPMA), the Bureau of Land Management (BLM) Palm Springs South Coast Field Office, Palm Springs, California, intends to prepare an Environmental Impact Statement (EIS) for the Rio Mesa Gen- Tie Project. The proposed project would require an......

2012-08-29

221

USDA Forest Service Gen. Tech. Rep. PSW-GTR-203. 2007.300 Prescribed Burning Ineffective for  

E-print Network

of the forest floor and from plentiful wind-blown seeds from nearby stands around the burned site. A secondUSDA Forest Service Gen. Tech. Rep. PSW-GTR-203. 2007.300 Prescribed Burning Ineffective for Improving Turkey Habitat on a Recently Regenerated Mesic Site in the Southern Appalachian Mountains1 W

Standiford, Richard B.

222

USDA Forest Service Gen. Tech. Rep. PSW-GTR-181. 2002. 869 Managing Coarse Woody Debris in British  

E-print Network

-term forest productivity (Stevens 1996) by improving soil-moisture retention, contributing to soil structure of coarse woody debris is highly variable in forest ecosystems and depends largely on stand history, standUSDA Forest Service Gen. Tech. Rep. PSW-GTR-181. 2002. 869 Managing Coarse Woody Debris in British

Standiford, Richard B.

223

OmniGen-AF supplementation modulated the physiological and acute phase responses of Brahman heifers to an endotoxin challenge  

Technology Transfer Automated Retrieval System (TEKTRAN)

This study examined the effect of feeding OmniGen-AF (OG; Prince Agri Products) on the physiological and acute phase responses (APR) of newly-weaned heifers to an endotoxin (lipopolysaccharide; LPS) challenge. Brahman heifers (n=24; 183±5 kilograms) from the Texas AgriLife Research Center in Overton...

224

HAMULONEMA GEN. NOV. FOR OSTERTAGIA KENYENSIS AND TELADORSAGIA HAMATA IN THE OSTERTAGIINE FAUNA (NEMATODA: TRICHOSTRONGYLOIDEA) FROM AFRICAN UNGULATES  

Technology Transfer Automated Retrieval System (TEKTRAN)

: Hamulonema gen. nov. is proposed for Teladorsagia hamata and Ostertagia kenyensis in the ostertagiine nematode fauna found in artiodactyl hosts from Africa. Monomorphic species representing this genus are characterized by a bilaterally symmetrical and parallel synlophe in males and females, a 2-...

225

JENUFA GEN. NOV.: A NEW GENUS OF COCCOID GREEN ALGAE (CHLOROPHYCEAE, INCERTAE SEDIS) PREVIOUSLY RECORDED BY ENVIRONMENTAL SEQUENCING1  

E-print Network

JENUFA GEN. NOV.: A NEW GENUS OF COCCOID GREEN ALGAE (CHLOROPHYCEAE, INCERTAE SEDIS) PREVIOUSLY of unicellular green algae from algal biofilms growing on tree bark in a Southeast Asian tropical rainforest: AU, approximately unbiased; BBM, Bold basal medium; CAUP, Culture Collection of algae at Charles

226

381USDA Forest Service Gen. Tech. Rep. PSW-GTR-160. 1997. Bird Communities in Grazed and  

E-print Network

for the stability of a large industry and for the maintenance of public values to be rendered by grazing lands381USDA Forest Service Gen. Tech. Rep. PSW-GTR-160. 1997. Bird Communities in Grazed and Ungrazed. Turner2 Abstract: Ten years of spot-mapping censuses in grazed and ungrazed plots at the San Joaquin

Standiford, Richard B.

227

Santa Fe Institute Working Paper 11-05-XXX arxiv.org:1105.XXXX [physics.gen-ph  

E-print Network

. Decomposing the State 8 B. -Machines 10 C. Finite State Automata 12 D. Reversibility Revisited 12 VI. Examples in stationary processes gen- erated by edge-emitting, finite-state hidden Markov models, paralleling, including those which have a finite number of recurrent causal states in one direc- tion, but an infinite

228

Evaluation of Mixed-Mode Data-Link Communications for NextGen 4DT and Equivalent Visual Surface Operations  

NASA Technical Reports Server (NTRS)

By 2025, U.S. air traffic is predicted to increase 3-fold and may strain the current air traffic management system, which may not be able to accommodate this growth. In response to this challenge, a revolutionary new concept has been proposed for U.S. aviation operations, termed the Next Generation Air Transportation System or NextGen. Many key capabilities are being identified to enable NextGen, including the use of data-link communications. Because NextGen represents a radically different approach to air traffic management and requires a dramatic shift in the tasks, roles, and responsibilities for the flight deck, there are numerous research issues and challenges that must be overcome to ensure a safe, sustainable air transportation system. Flight deck display and crew-vehicle interaction concepts are being developed that proactively investigate and overcome potential technology and safety barriers that might otherwise constrain the full realization of NextGen. The paper describes simulation research examining data-link communications during 4DT and equivalent visual surface operations.

Prinzel, Lawrence J., III; Shelton, Kevin J.; Jones, Denise R.; Allamandola, Angela S.; Arthur, Jarvis, J., III; Bailey, Randall E.

2010-01-01

229

USDA Forest Service Gen. Tech. Rep. PSW-GTR-203. 2007. 261 Delayed Conifer Tree Mortality Following  

E-print Network

for incense-cedar, Jeffrey pine, ponderosa pine, red fir and white fir. Two-year post-fire preliminary modelsUSDA Forest Service Gen. Tech. Rep. PSW-GTR-203. 2007. 261 Delayed Conifer Tree Mortality Following was characterized and survival monitored for 5,246 trees from five wildfires in California that occurred between

Standiford, Richard B.

230

USDA Forest Service Gen. Tech. Rep. PSW-GTR-181. 2002. 23 Dead Wood Dynamics in Stream  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-GTR-181. 2002. 23 Dead Wood Dynamics in Stream Ecosystems1 Robert J. Naiman,2 Estelle V. Balian,2 Krista K. Bartz,2 Robert E. Bilby,3 and Joshua J. Latterell2 at the Symposium on the Ecology and Management of Dead Wood in Western Forests, November 2-4, 1999, Reno, Nevada. 2

Standiford, Richard B.

231

139USDA Forest Service Gen. Tech. Rep. PSW-GTR-158. 1995. The Role of Fire in Ecosystem Management1  

E-print Network

139USDA Forest Service Gen. Tech. Rep. PSW-GTR-158. 1995. The Role of Fire in Ecosystem Management1, in part or collectively, changed many forests. However well- meaning at the time, in some ecosystems the role of fire in ecosystem management and it answers these three questions: 1) what is ecosystem manage

Standiford, Richard B.

232

Rationale and Design of the National Registry of Genetically Triggered Thoracic Aortic Aneurysms and Cardiovascular Conditions (GenTAC) Registry  

PubMed Central

Background Although the management of thoracic aortic aneurysms (TAAs) has improved significantly, patients presenting with aortic dissections, rupture or other acute complications of TAAs continue to suffer high rates of morbidity and mortality. Accumulating data have indicated that many TAAs are due to underlying gene mutations. A comprehensive approach to the study of TAAs resulting from genetic mutations is needed to translate this information into advances in treatment. Objective The National Registry of Genetically Triggered Thoracic Aortic Aneurysms and Cardiovascular Conditions (GenTAC) was established to provide a biospecimen inventory and bioinformatics infrastructure to enable research to advance the clinical management of genetically-triggered TAAs and related complications. Methods The GenTAC Registry is a longitudinal observational cohort study enrolling patients with conditions related to genetically-induced TAAs from five regional clinical centers in the United States. Results Over 700 subjects with associated clinical histories, physical examinations, imaging data, and biospecimens have been enrolled in the Registry to date. Enrollment is expected to continue until September 2010. Total enrollment of nearly 3,000 subjects is expected. No interim analysis has yet been undertaken. Conclusions GenTAC has been established to facilitate studies by GenTAC investigators and others that will advance multiple scientific frontiers in thoracic aortic disease. Genotypic, proteomic, clinical, and imaging data will be integrated systematically with outcomes data to determine the optimal clinical management of patients suffering from genetically-induced TAAs. PMID:19185640

2010-01-01

233

USDA Forest Service Gen. Tech. Rep. PSW-GTR-193. 2004. 65 Fire in the Sierra Nevada1  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-GTR-193. 2004. 65 Fire in the Sierra Nevada1 Carl N for long-term functioning of Sierra Nevada ecosystems and as one of the greatest threats to human and natural resources (SNEP 1996a). Fire has shaped the terrestrial ecosystems of the Sierra Nevada

Stephens, Scott L.

234

USDA Forest Service Gen. Tech. Rep. PSW-GTR-181. 2002. 823 Effects of Prescribed Fire in Ponderosa  

E-print Network

) in northern Arizona ponderosa pine (Pinus ponderosa) forests. Old-growth ponderosa pine trees are used by 80USDA Forest Service Gen. Tech. Rep. PSW-GTR-181. 2002. 823 Effects of Prescribed Fire in Ponderosa-Parker2 and Richard Miller3 Abstract We monitored the effects of prescribed fire in ponderosa pine forest

Standiford, Richard B.

235

PSeq-Gen: an application for the Monte Carlo simulation of protein sequence evolution along phylogenetic trees  

Microsoft Academic Search

Motivation: Seq-Gen is a program that will simulate the evolution of nucleotide sequences along a phylogeny, using common models of the substitution process. A range of models of molecular evolution are implemented, including the general reversible model. Nucleotide frequencies and other parameters of the model may be given and site-specific rate heterogeneity can also be incorporated in a number of

Andrew Rambaut; J. Adachi

1997-01-01

236

78 FR 26004 - Notice of Availability; Draft Environmental Impact Statement for the FutureGen 2.0 Project  

Federal Register 2010, 2011, 2012, 2013, 2014

...session will begin at approximately 6:00 p.m. DOE will begin the hearing's formal session with overviews of its clean coal program, proposed FutureGen 2.0 Project, and the NEPA process, followed by oral statements by pre- registered...

2013-05-03

237

75USDA Forest Service Gen. Tech. Rep. PSW-GTR-160. 1997. The Influence of Epiphytic Lichens on the  

E-print Network

75USDA Forest Service Gen. Tech. Rep. PSW-GTR-160. 1997. The Influence of Epiphytic Lichens Abstract: We evaluated the importance of epiphytic lichens in the nutrient cycling of a blue oak (Quercus douglasii) woodland in California. Each oak tree contained an average of 3.8 kg lichen biomass, totaling 590

Standiford, Richard B.

238

ExGen 500 is an efficient vector for gene delivery to lung epithelial cells in vitro and in vivo  

Microsoft Academic Search

Nonviral vectors might represent a safe alternative to adenovirus for gene therapy of lung disorders, in particular cystic fibrosis (CF). Cationic lipids have been shown to correct the CF defect both in vitro and in vivo, but more efficient vectors are needed to improve the low gene transfer efficiency. Here, we show that the cationic polymer ExGen 500, a linear

S Ferrari; E Moro; A Pettenazzo; JP Behr; F Zacchello; M Scarpa

1997-01-01

239

USDA Forest Service Gen. Tech. Rep. PSW-GTR-193. 2004. 25 Session Overview: Climate and Landscape  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-GTR-193. 2004. 25 Session Overview: Climate and Landscape on familiar themes, our goal in the session "Climate and Landscape Change Over Time" was to introduce a topic understanding about the functioning and dynamics of the climate system. Second, these new insights are relevant

Standiford, Richard B.

240

USDA Forest Service Gen. Tech. Rep. PSW-GTR-181. 2002. 369 Assemblages of Vascular Plants on Logs  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-GTR-181. 2002. 369 Assemblages of Vascular Plants on Logs and wildfire stands. Logs and stumps were colonized by a significantly different assemblage of vascular plants were similar. However, as dead wood decayed, assemblages of vascular plants diverged and became more

Standiford, Richard B.

241

109USDA Forest Service Gen. Tech. Rep. PSW-GTR-160. 1997. Patterns and Processes of Adaptation  

E-print Network

109USDA Forest Service Gen. Tech. Rep. PSW-GTR-160. 1997. Patterns and Processes of Adaptation suggest local scale adaptation. Differences among maternal families in survival and growth were that natural selection has resulted in the formation of genetically differentiated populations adapted to local

Standiford, Richard B.

242

85USDA Forest Service Gen. Tech. Rep. PSW-GTR-158. 1995. Structure Ignition Assessment Model (SIAM)1  

E-print Network

85USDA Forest Service Gen. Tech. Rep. PSW-GTR-158. 1995. Structure Ignition Assessment Model (SIAM mechanisms are not well known on how structures ignite in association with wildland fires. In response to the need for a better understanding of wildland/urban interface ignition mecha- nisms and a method

Standiford, Richard B.

243

267USDA Forest Service Gen. Tech. Rep. PSW-GTR-160. 1997. Rehabilitation of a Blue Oak  

E-print Network

267USDA Forest Service Gen. Tech. Rep. PSW-GTR-160. 1997. Rehabilitation of a Blue Oak Restoration. Results indicate treeshelters release stunted seedlings and could rehabilitate poorly performing projects attempted to rehabilitate our original planting, in order to evaluate readily available measures applicable

Standiford, Richard B.

244

Redesigning Services for the Net-Gen and Beyond: A Holistic Review of Pedagogy, Resource, and Learning Space  

Microsoft Academic Search

Oblinger and Oblinger (2005) described the Net Generation or Net Gen, who have never known life without the Internet. They note the incremental nature of change: “One generation's technology is taken for granted by the next” (p. 2.1). In a world increasingly dominated by technology, the academic library has become a place to learn that is constantly adapting and changing,

Jill Beard; Penny Dale

2008-01-01

245

U.S. ARMY CORPS OF ENGINEERS NORTH ATLANTIC DIVISION 302 Gen. Lee Ave., Brooklyn, NY 11252  

E-print Network

U.S. ARMY CORPS OF ENGINEERS ­ NORTH ATLANTIC DIVISION 302 Gen. Lee Ave., Brooklyn, NY 11252 www projects to reduce the risk of future coastal storm damage. In the Corps' North Atlantic Division to a need to secure funding, a lack of easements, or both. Of the 18 projects in the North Atlantic Division

US Army Corps of Engineers

246

1USDA Forest Service Gen. Tech. Report PSW-GTR-163. 1997. Photo Series for Quantifying Fuels and  

E-print Network

1USDA Forest Service Gen. Tech. Report PSW-GTR-163. 1997. Photo Series for Quantifying Fuels. Fire is essential to the continued vigor of this type and plays many roles ranging from fuel reduction, effective fire exclusion in this century has caused dead and live fuel loadings to increase in many groves

Standiford, Richard B.

247

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 271 Mortality of Marbled Murrelets in Gill Nets in North America  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 271 Chapter 27 Mortality of Marbled Murrelets: Mortality of Marbled Murrelets (Brachyramphus marmoratus) due to accidental capture in gill nets is one the impacts of gill-net mortality on the Marbled Murrelet (Brachyramphus marmoratus) (Carter and Morrison 1992

Standiford, Richard B.

248

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 371 Productivity of Marbled Murrelets in California from  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 371 Chapter 35 Productivity of Marbled Murrelets an intensive survey method in 1993 to identify juvenile Marbled Murrelets (Brachyramphus marmoratus) at sea a demographic model of any species is a measure of that species' productivity. In the case of the Marbled

Standiford, Richard B.

249

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 295 Abstract: About 45,000-50,000 Marbled Murrelets (Brachyramphus  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 295 Abstract: About 45,000-50,000 Marbled in the population size of Marbled Murrelets and also in complexity of marine habitats used by these birds of the Marbled Murrelet obtained largely from boat surveys. Abundance and Distribution Regional and Range

Standiford, Richard B.

250

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 3 Ecology and Conservation of the Marbled Murrelet in  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 3 Ecology and Conservation of the Marbled4 Chapter 1 Abstract: Over the past decade, the Marbled Murrelet has become a focus of much Service in February 1993. In order to aid the various agencies with management, the Marbled Murrelet

Standiford, Richard B.

251

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 327 Abundance and Distribution of Marbled Murrelets in Oregon  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 327 Abundance and Distribution of Marbled Abstract: To determine the abundance and distribution of Marbled Murrelets, aerial surveys of the Oregon and distribution is an important element in the conservation management of the Marbled Murrelet (Brachyramphus

Standiford, Richard B.

252

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 223 Marbled Murrelet Food Habits and Prey Ecology  

E-print Network

USDA Forest Service Gen. Tech. Rep. PSW-152. 1995. 223 Marbled Murrelet Food Habits and Prey Ecology Esther E. Burkett1 Abstract: Information on food habits of the Marbled Murrelet (Brachyramphus with El Niño events, could cause population declines and even extirpation. An understanding of Marbled

Standiford, Richard B.

253

USDA Forest Service Gen. Tech. Rep. PSW-GTR-198. 2005. 123 Management of Ponderosa Pine Nutrition  

E-print Network

of the Intermountain Forest Tree Nutrition Cooperative, Forest Resources Department, College of Natural ResourcesUSDA Forest Service Gen. Tech. Rep. PSW-GTR-198. 2005. 123 Management of Ponderosa Pine Nutrition. Lawson) nutrition. Ponderosa pine nitrogen (N) status was often better than the N-status of other Inland

Standiford, Richard B.

254

204 USDA Forest Service Gen. Tech. Rep. RMRS-GTR-114. 2003 Part 3: Soil Properties, Erosion,  

E-print Network

-third by wind. In forested areas erosion can occur by a wide variety of processes, including soil creep, dry204 USDA Forest Service Gen. Tech. Rep. RMRS-GTR-114. 2003 Part 3: Soil Properties, ErosionDonald, and John Norman Introduction This team was asked to address three questions regarding soil properties

255

Sandrewia, n. gen., a problematical plant from the Lower Permian of Texas and Kansas  

USGS Publications Warehouse

Sandrewia, n. gen., monotypified by S. texana, n. sp., is a plant from Lower Permian beds of north-central Texas and east-central Kansas. It is characterized by stout axes with spirally disposed, laxly inserted, petiolate leaves; the laminae are broadly flabelliform with coarse, open venation. The leaves are reminiscent of the vojnovskyalean Nephropsis, of the Permian Petchora Basin, U.S.S.R., but biologic relationships are only speculative because of limited material. However, leaf characteristics render Sandrewia easily identifiable. Its presently limited stratigraphic range, along with floristic associations, indicates it may be a useful guide fossil and supports the author's beliefs regarding important times and places in Paleozoic plant evolution. ?? 1975.

Mamay, S.H.

1975-01-01

256

The Neotropical genus Ginungagapus gen. nov. (Hesperiidae, Hesperiinae, Moncini): phylogenetic position and taxonomic review.  

PubMed

A taxonomic and phylogenetic study based on morphological characters revealed the type species of the genus Lucida Evans, 1955, Lucida lucia (Capronnier, 1874) to be paraphyletic to other species of the genus. As a result, a new genus, Ginungagapus gen. nov., is erected including four species previously placed in Lucida: Ginungagapus schmithi (Bell, 1930) comb. nov., Ginungagapus bocus (Bell, 1947) comb. nov., Ginungagapus ranesus (Schaus, 1902) comb. nov., and Ginungagapus rogan (Evans, 1955) comb. nov.; and four new species are added: Ginungagapus awarreni, sp. nov., Ginungagapus tangerinii, sp. nov., Ginungagapus fiedleri, sp. nov. and Ginungagapus brasilia, sp. nov. The new genus is taxonomically revised, genitalia are illustrated, except of the female of G. rogan (Evans, 1955) and of the male of G. brasilia, sp. nov., which are still unknown. An identification key is provided. PMID:25781822

Carneiro, Eduardo

2015-01-01

257

Application of constitutive model considering nonlinear unloading behavior for Gen.3 AHSS  

NASA Astrophysics Data System (ADS)

Nonlinear unloading behavior has been reported as an important factor for accurate springback prediction. In this study, a newly proposed special component of strain: "Quasi-Plastic-Elastic" ("QPE") strain was utilized to study the springback behavior of Advanced High Strength Steels (AHSS). Several types of steels, including IF steel, DP780, TRIP780, DP980, TWIP980 and QP980 were considered in this research. The results showed that all the tested steels have following behavior: 1) QPE strain is recoverable, like elastic deformation. 2) It dissipates work, like plastic deformation. A 3-D constitutive model considering QPE behavior was implemented in Abaqus/Standard with shell element and applied to draw-bend springback test for Gen. 3 AHSS, QP980. Predictions for springback using the QPE model were more accurate compared with standard elastic-plastic models.

Sun, Li; Wagoner, R. H.

2013-05-01

258

Combresomyces cornifer gen. sp. nov., an endophytic peronosporomycete in Lepidodendron from the Carboniferous of central France.  

PubMed

Structurally preserved periderm of the lycophyte Lepidodendron rhodumnense from the Visean (Mississippian) of central France contains a peronosporomycete (Combresomyces cornifer gen. sp. nov.) that occurs in the form of pyriform to subglobose terminal oogonia. On the surface is a conspicuous ornamentation, which may have formed through condensation of a mucilaginous extra-oogonial wall secretion. Some oogonia contain thin-walled spherules, which may represent (walled) oospheres or spores of an endoparasitic fungus (?chytrid), whereas single, large spheres in the interior are interpreted as oospores. Antheridia adpressed to several of the specimens are clavate and paragynous. This discovery sheds light on the morphology and biology of peronosporomycetes in a terrestrial ecosystem some 330My ago. Although the organism occurs exclusively in the periderm of L. rhodumnense, it is not known whether it represents a symptomless endophyte, pathogen, or saprotroph. PMID:18692373

Dotzler, Nora; Krings, Michael; Agerer, Reinhard; Galtier, Jean; Taylor, Thomas N

2008-09-01

259

IceCube-Gen2: A Vision for the Future of Neutrino Astronomy in Antarctica  

E-print Network

The recent observation by the IceCube neutrino observatory of an astrophysical flux of neutrinos represents the "first light" in the nascent field of neutrino astronomy. The observed diffuse neutrino flux seems to suggest a much larger level of hadronic activity in the non-thermal universe than previously thought and suggests a rich discovery potential for a larger neutrino observatory. This document presents a vision for an substantial expansion of the current IceCube detector, IceCube-Gen2, including the aim of instrumenting a $10\\,\\mathrm{km}^3$ volume of clear glacial ice at the South Pole to deliver substantial increases in the astrophysical neutrino sample for all flavors. A detector of this size would have a rich physics program with the goal to resolve the sources of these astrophysical neutrinos, discover GZK neutrinos, and be a leading observatory in future multi-messenger astronomy programs.

IceCube-Gen2 Collaboration; :; M. G. Aartsen; M. Ackermann; J. Adams; J. A. Aguilar; M. Ahlers; M. Ahrens; D. Altmann; T. Anderson; G. Anton; C. Arguelles; T. C. Arlen; J. Auffenberg; S. Axani; X. Bai; I. Bartos; S. W. Barwick; V. Baum; R. Bay; J. J. Beatty; J. Becker Tjus; K. -H. Becker; S. BenZvi; P. Berghaus; D. Berley; E. Bernardini; A. Bernhard; D. Z. Besson; G. Binder; D. Bindig; M. Bissok; E. Blaufuss; J. Blumenthal; D. J. Boersma; C. Bohm; F. Bos; D. Bose; S. Böser; O. Botner; L. Brayeur; H. -P. Bretz; A. M. Brown; N. Buzinsky; J. Casey; M. Casier; E. Cheung; D. Chirkin; A. Christov; B. Christy; K. Clark; L. Classen; F. Clevermann; S. Coenders; G. H. Collin; J. M. Conrad; D. F. Cowen; A. H. Cruz Silva; J. Daughhetee; J. C. Davis; M. Day; J. P. A. M. de André; C. De Clercq; S. De Ridder; P. Desiati; K. D. de Vries; M. de With; T. DeYoung; J. C. Dí andaz-Vélez; M. Dunkman; R. Eagan; B. Eberhardt; T. Ehrhardt; B. Eichmann; J. Eisch; S. Euler; J. J. Evans; P. A. Evenson; O. Fadiran; A. R. Fazely; A. Fedynitch; J. Feintzeig; J. Felde; K. Filimonov; C. Finley; T. Fischer-Wasels; S. Flis; K. Frantzen; T. Fuchs; T. K. Gaisser; R. Gaior; J. Gallagher; L. Gerhardt; D. Gier; L. Gladstone; T. Glüsenkamp; A. Goldschmidt; G. Golup; J. G. Gonzalez; J. A. Goodman; D. Góra; D. Grant; P. Gretskov; J. C. Groh; A. Groß; C. Ha; C. Haack; A. Haj Ismail; P. Hallen; A. Hallgren; F. Halzen; K. Hanson; J. Haugen; D. Hebecker; D. Heereman; D. Heinen; K. Helbing; R. Hellauer; D. Hellwig; S. Hickford; J. Hignight; G. C. Hill; K. D. Hoffman; R. Hoffmann; A. Homeier; K. Hoshina; F. Huang; W. Huelsnitz; P. O. Hulth; K. Hultqvist; A. Ishihara; E. Jacobi; J. Jacobsen; G. S. Japaridze; K. Jero; O. Jlelati; B. J. P. Jones; M. Jurkovic; O. Kalekin; A. Kappes; T. Karg; A. Karle; T. Katori; U. F. Katz; M. Kauer; A. Keivani; J. L. Kelley; A. Kheirandish; J. Kiryluk; J. Kläs; S. R. Klein; J. -H. Köhne; G. Kohnen; H. Kolanoski; A. Koob; L. Köpke; C. Kopper; S. Kopper; D. J. Koskinen; M. Kowalski; C. B. Krauss; A. Kriesten; K. Krings; G. Kroll; M. Kroll; J. Kunnen; N. Kurahashi; T. Kuwabara; M. Labare; J. L. Lanfranchi; D. T. Larsen; M. J. Larson; M. Lesiak-Bzdak; M. Leuermann; J. LoSecco; J. Lünemann; J. Madsen; G. Maggi; K. B. M. Mahn; S. Marka; Z. Marka; R. Maruyama; K. Mase; H. S. Matis; R. Maunu; F. McNally; K. Meagher; M. Medici; A. Meli; T. Meures; S. Miarecki; E. Middell; E. Middlemas; N. Milke; J. Miller; L. Mohrmann; T. Montaruli; R. W. Moore; R. Morse; R. Nahnhauer; U. Naumann; H. Niederhausen; S. C. Nowicki; D. R. Nygren; A. Obertacke; S. Odrowski; A. Olivas; A. Omairat; A. O'Murchadha; T. Palczewski; L. Paul; Ö. Penek; J. A. Pepper; C. Pérez de los Heros; C. Pfendner; D. Pieloth; E. Pinat; J. L. Pinfold; J. Posselt; P. B. Price; G. T. Przybylski; J. Pütz; M. Quinnan; L. Rädel; M. Rameez; K. Rawlins; P. Redl; I. Rees; R. Reimann; M. Relich; E. Resconi; W. Rhode; M. Richman; B. Riedel; S. Robertson; J. P. Rodrigues; M. Rongen; C. Rott; T. Ruhe; B. Ruzybayev; D. Ryckbosch; S. M. Saba; H. -G. Sander; J. Sandroos; P. Sandstrom; M. Santander; S. Sarkar; K. Schatto; F. Scheriau; T. Schmidt; M. Schmitz; S. Schoenen; S. Schöneberg; A. Schönwald; A. Schukraft; L. Schulte; O. Schulz; D. Seckel; Y. Sestayo; S. Seunarine; M. H. Shaevitz; R. Shanidze; M. W. E. Smith; D. Soldin; S. Söldner-Rembold; G. M. Spiczak; C. Spiering; M. Stamatikos; T. Stanev; N. A. Stanisha; A. Stasik; T. Stezelberger; R. G. Stokstad; A. Stöß andl; E. A. Strahler; R. Ström; N. L. Strotjohann; G. W. Sullivan; H. Taavola; I. Taboada; A. Taketa; A. Tamburro; H. K. M. Tanaka; A. Tepe; S. Ter-Antonyan; A. Terliuk; G. Teš; andi?; S. Tilav; P. A. Toale; M. N. Tobin; D. Tosi; M. Tselengidou; E. Unger; M. Usner; S. Vallecorsa; N. van Eijndhoven; J. Vandenbroucke; J. van Santen; S. Vanheule; M. Vehring; M. Voge; M. Vraeghe; C. Walck; M. Wallraff; Ch. Weaver; M. Wellons; C. Wendt; S. Westerhoff; B. J. Whelan; N. Whitehorn; C. Wichary; K. Wiebe; C. H. Wiebusch; D. R. Williams; H. Wissing; M. Wolf; T. R. Wood; K. Woschnagg; S. Wren; D. L. Xu; X. W. Xu; Y. Xu; J. P. Yanez; G. Yodh; S. Yoshida; P. Zarzhitsky; J. Ziemann; M. Zoll

2014-12-18

260

Eremonidiopsis aggregata, gen. n., sp. n. from Cuba, the third West Indian Dioptinae (Lepidoptera, Notodontidae)  

PubMed Central

Abstract A new genus and species of Dioptinae (Lepidoptera, Noctuoidea, Notodontidae) is described from Cuba, this being the third taxon of the subfamily known from the West Indies. Eremonidiopsis aggregata, gen. n., sp. n., appears to be closely related to Eremonidia mirifica Rawlins & Miller from Hispaniola among members of the tribe Dioptini. Eremonidiopsis aggregata is known from two localities in the middle and western portions of the northeastern Cuban mountain range, Nipe–Sagua–Baracoa. The species inhabits low elevations (300–400 m) covered by lowland rainforest and sclerophyll rainforest. The six known specimens, all males, were part of small swarms flying near the top of an unidentified tree during the day at both collecting sites. These localities are included within protected areas, the “Pico Cristal” National Park in the West and the “Alexander von Humbolt” National Park in the East. PMID:24146561

Aguila, Rayner Núñez

2013-01-01

261

The haemosporidian parasites of bats with description of Sprattiella alecto gen. nov., sp. nov.  

PubMed Central

Four species of Haemoproteidae were found in Pteropus alecto Temminck, 1837 in Queensland, Australia: i) Johnsprentia copemani, Landau et al., 2012; ii) Sprattiella alecto gen. nov., sp. nov., characterised by schizonts in the renal vessels; iii) Hepatocystis levinei, Landau et al., 1985, originally described from Pteropus poliocephalus Temminck, 1825 and, experimentally from Culicoides nubeculosus and found in this new host and for which features of the hepatic schizonts are reported; iv) gametocytes of Hepatocystis sp. which are illustrated but cannot be assigned to a known species. A tentative interpretation of phylogenetic characters of haemosporidians of bats is provided from the morphology of the gametocytes and localisation of the tissue stages with respect to recent data on the phylogeny of bats. PMID:22550624

Landau, I.; Chavatte, J.M.; Karadjian, G.; Chabaud, A.; Beveridge, I.

2012-01-01

262

NASA System-Level Design, Analysis and Simulation Tools Research on NextGen  

NASA Technical Reports Server (NTRS)

A review of the research accomplished in 2009 in the System-Level Design, Analysis and Simulation Tools (SLDAST) of the NASA's Airspace Systems Program is presented. This research thrust focuses on the integrated system-level assessment of component level innovations, concepts and technologies of the Next Generation Air Traffic System (NextGen) under research in the ASP program to enable the development of revolutionary improvements and modernization of the National Airspace System. The review includes the accomplishments on baseline research and the advancements on design studies and system-level assessment, including the cluster analysis as an annualization standard of the air traffic in the U.S. National Airspace, and the ACES-Air MIDAS integration for human-in-the-loop analyzes within the NAS air traffic simulation.

Bardina, Jorge

2011-01-01

263

Coynema gen. n., a new genus of nematode (Thelastomatoidea, Hystrignathidae) parasites of Passalidae (Coleoptera) from Cuba  

PubMed Central

Abstract The new genus Coynema gen. n. is described as parasite of the two passalid beetles from Cuba: Passalus interstitialis Escholtz, 1829 (type host) and Passalus pertyi Kaup, 1869. Females are characterized by the shape of their cephalic end, cervical cuticle unarmed, a sub-cylindrical procorpus with its base abruptly dilated, fore region of intestine dilated as a sac-like structure, genital system didelphic-amphidelphic and eggs markedly ovoid and smooth-shelled. Males have a digestive system similar to females, tail sharply pointed, bearing a Y-like thickening of the dorsal cuticle. They also present a big, median, mammiform pre-cloacal papillae and a pair of small, sub-dorsal pre-cloacal papillae anterior to the cuticular thickening of the tail. PMID:21594137

Rodríguez, Jans Morffe; Rodríguez, Nayla García

2011-01-01

264

Rugosothynnus gen. nov. (Hymenoptera: Tiphiidae: Thynninae: Rhagigasterini), a newly recognised Australian genus.  

PubMed

Rugosothynnus gen. nov. (type species Rhagigaster corrugatus Turner, 1910) is erected for 12 Rhagigaster-like species. Nine species are described and illustrated from Australia: R. brunneus sp. nov., R. clypeatus sp. nov., R. confusus sp. nov., R. depressus sp. nov., R. fulvescens sp. nov., R. houstoni sp. nov., R. monteithae sp. nov., R. neocorrugatus sp. nov. and R. schichai sp. nov. New combinations are proposed for R. burnsi (Given, 1959), comb. nov., R. corrugatus (Turner, 1910), comb. nov. and R. tristis (Smith, 1859), comb. nov. (all previously from genus Rhagigaster Guérin-Ménéville, 1838). A key to both sexes of all species is provided except for the females of R. clypeatus sp. nov., R. depressus sp. nov., R. neocorrugatus sp. nov. and R. schichai sp. nov. which are unknown. PMID:25781749

Brown, Graham

2015-01-01

265

Enhanced Vision for All-Weather Operations Under NextGen  

NASA Technical Reports Server (NTRS)

Recent research in Synthetic/Enhanced Vision technology is analyzed with respect to existing Category II/III performance and certification guidance. The goal is to start the development of performance-based vision systems technology requirements to support future all-weather operations and the NextGen goal of Equivalent Visual Operations. This work shows that existing criteria to operate in Category III weather and visibility are not directly applicable since, unlike today, the primary reference for maneuvering the airplane is based on what the pilot sees visually through the "vision system." New criteria are consequently needed. Several possible criteria are discussed, but more importantly, the factors associated with landing system performance using automatic and manual landings are delineated.

Bailey, Randall E.; Kramer, Lynda J.; Williams, Steven P.; Bailey, Randall E.; Kramer, Lynda J.; Williams, Steven P.

2010-01-01

266

Multimodal information Management: Evaluation of Auditory and Haptic Cues for NextGen Communication Displays  

NASA Technical Reports Server (NTRS)

Auditory communication displays within the NextGen data link system may use multiple synthetic speech messages replacing traditional ATC and company communications. The design of an interface for selecting amongst multiple incoming messages can impact both performance (time to select, audit and release a message) and preference. Two design factors were evaluated: physical pressure-sensitive switches versus flat panel "virtual switches", and the presence or absence of auditory feedback from switch contact. Performance with stimuli using physical switches was 1.2 s faster than virtual switches (2.0 s vs. 3.2 s); auditory feedback provided a 0.54 s performance advantage (2.33 s vs. 2.87 s). There was no interaction between these variables. Preference data were highly correlated with performance.

Begault, Durand R.; Bittner, Rachel M.; Anderson, Mark R.

2012-01-01

267

Biological Sequence Simulation for Testing Complex Evolutionary Hypotheses: indel-Seq-Gen Version 2.0  

PubMed Central

Sequence simulation is an important tool in validating biological hypotheses as well as testing various bioinformatics and molecular evolutionary methods. Hypothesis testing relies on the representational ability of the sequence simulation method. Simple hypotheses are testable through simulation of random, homogeneously evolving sequence sets. However, testing complex hypotheses, for example, local similarities, requires simulation of sequence evolution under heterogeneous models. To this end, we previously introduced indel-Seq-Gen version 1.0 (iSGv1.0; indel, insertion/deletion). iSGv1.0 allowed heterogeneous protein evolution and motif conservation as well as insertion and deletion constraints in subsequences. Despite these advances, for complex hypothesis testing, neither iSGv1.0 nor other currently available sequence simulation methods is sufficient. indel-Seq-Gen version 2.0 (iSGv2.0) aims at simulating evolution of highly divergent DNA sequences and protein superfamilies. iSGv2.0 improves upon iSGv1.0 through the addition of lineage-specific evolution, motif conservation using PROSITE-like regular expressions, indel tracking, subsequence-length constraints, as well as coding and noncoding DNA evolution. Furthermore, we formalize the sequence representation used for iSGv2.0 and uncover a flaw in the modeling of indels used in current state of the art methods, which biases simulation results for hypotheses involving indels. We fix this flaw in iSGv2.0 by using a novel discrete stepping procedure. Finally, we present an example simulation of the calycin-superfamily sequences and compare the performance of iSGv2.0 with iSGv1.0 and random model of sequence evolution. PMID:19651852

Strope, Cory L.; Abel, Kevin; Scott, Stephen D.

2009-01-01

268

Parablastomonas arctica gen. nov., sp. nov., isolated from high Arctic glacial till.  

PubMed

A pale yellow, aerobic bacterium, strain M0-2(T), was isolated from a till sample. Its taxonomic position was investigated by using a polyphasic approach. Cells were Gram-stain-negative, rod-shaped and motile. Cells reproduced by budding or asymmetrical cell division. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M0-2(T) belonged to the family Sphingomonadaceae and was closely related to species of the genera Novosphingobium (96.4-92.0%) and Blastomonas (94.6%), Sphingopyxis witflariensis W-50(T) (94.0%), Sphingosinicella soli KSL-125(T) (93.6%) and Sphingomonas astaxanthinifaciens TDMA-17(T) (93.5%). Ubiquinone-10 (Q-10) was the predominant respiratory quinone. The major fatty acids were summed feature 8 (comprising C18:1?7c and/or C18:1?6c, 31.9%), summed feature 3 (comprising C16:1?7c and/or C16:1?6c, 19.8%) and C14:0 2-OH (8.9%). Sphingoglycolipid, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine were the major polar lipids. Spermidine was the major polyamine observed in the cell. The genomic DNA G+C content was 47.5 mol%. On the basis of phylogenetic relationships and the low DNA G+C content compared with most other genera of the family Sphingomonadaceae, combined with phenotypic and chemotaxonomic data, strain M0-2(T) is considered to represent a novel species in a new genus in the family Sphingomonadaceae for which the name Parablastomonas arctica gen. nov., sp. nov. is proposed. The type strain of Parablastomonas arctica gen. nov., sp. nov. is M0-2(T) (?=CCTCC AB 2012968(T)?=?NRRL B-59110(T)). PMID:25336722

Ren, Lvzhi; Chang, Xulu; Jiang, Fan; Kan, Wenjing; Qu, Zhihao; Qiu, Xia; Fang, Chengxiang; Peng, Fang

2015-01-01

269

Pseudochelatococcus lubricantis gen. nov., sp. nov. and Pseudochelatococcus contaminans sp. nov. from coolant lubricants.  

PubMed

Two Gram-negative, rod-shaped, non-spore-forming bacteria, isolated from metal working fluids were investigated to determine their taxonomic positions. On the basis of 16S rRNA gene sequence phylogeny, both strains (MPA 1113(T) and MPA 1105(T)) formed a distinct cluster with 97.7?% sequence similarity between them, which was in the vicinity of members of the genera Methylobacterium, Camelimonas, Chelatococcus, Bosea, Salinarimonas and Microvirga to which they showed low sequence similarities (below 94?%). The predominant compounds in the polyamine pattern and in the quinone system of the two strains were spermidine and ubiquinone Q-10, respectively. The polar lipid profiles were composed of the major compounds: phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine, major or moderate amounts of diphosphatidylglycerol, two unidentified glycolipids and three unidentified aminolipids. Several minor lipids were also detected. The major fatty acids were either C19?:?0 cyclo ?8c or C18?:?1?7c. The results of fatty acid analysis and physiological and biochemical tests allowed both, the genotypic and phenotypic differentiation of the isolates from each other, while the chemotaxonomic traits allowed them to be differentiated from the most closely related genera. In summary, low 16S rRNA gene sequence similarities and marked differences in polar lipid profiles, as well as in polyamine patterns, is suggestive of a novel genus for which the name Pseudochelatococcus gen. nov. is proposed. MPA 1113(T) (?=?CCM 8528(T)?=?LMG 28286(T)?=?CIP 110802(T)) and MPA 1105(T) (?=?CCM 8527(T)?=?LMG 28285(T)) are proposed to be the type strains representing two novel species within the novel genus, Pseudochelatococcus gen. nov., for which the names Pseudochelatococcus lubricantis sp. nov. and Pseudochelatococcus contaminans sp. nov. are suggested, respectively. PMID:25301542

Kämpfer, Peter; Glaeser, Stefanie P; Gräber, Marco; Rabenstein, Andreas; Kuever, Jan; Busse, Hans-Jürgen

2015-01-01

270

MolliGen, a database dedicated to the comparative genomics of Mollicutes.  

PubMed

Bacteria belonging to the class Mollicutes were among the first ones to be selected for complete genome sequencing because of the minimal size of their genomes and their pathogenicity for humans and a broad range of animals and plants. At this time six genome sequences have been publicly released (Mycoplasma genitalium, Mycoplasma pneumoniae, Ureaplasma urealyticum-parvum, Mycoplasma pulmonis, Mycoplasma penetrans and Mycoplasma gallisepticum) and as the number of available mollicute genomes increases, comparative genomics analysis within this model group of organisms becomes more and more instructive. However, such an analysis is difficult to carry out without a suitable platform gathering not only the original annotations but also relevant information available in public databases or obtained by applying common bioinformatics methods. With the aim of solving these difficulties, we have developed a web-accessible database named MolliGen (http://cbi.labri.fr/outils/molligen/). After selecting a set of genomes the user can launch various types of search based on annotation, position on the chromosomes or sequence similarity. In addition, relationships of putative orthology have been precomputed to allow differential genome queries. The results are presented in table format with multiple links to public databases and to bioinformatic analyses such as multiple alignments or BLAST search. Specific tools were also developed for the graphical visualization of the results, including a multi- genome browser for displaying dynamic pictures with clickable objects and for viewing relationships of precomputed similarity. MolliGen is designed to integrate all the complete genomes of mollicutes as they become available. PMID:14681420

Barré, Aurélien; de Daruvar, Antoine; Blanchard, Alain

2004-01-01

271

Aggregicoccus edonensis gen. nov., sp. nov., an unusually aggregating myxobacterium isolated from a soil sample.  

PubMed

A novel myxobacterium, MCy1366(T) (Ar1733), was isolated in 1981 from a soil sample collected from a region near Tokyo, Japan. It displayed general myxobacterial features like Gram-negative-staining, rod-shaped vegetative cells, gliding on solid surfaces, microbial lytic activity, fruiting-body-like aggregates and myxospore-like structures. The strain was mesophilic, aerobic and showed a chemoheterotrophic mode of nutrition. It was resistant to many antibiotics such as cephalosporin C, kanamycin, gentamicin, hygromycin B, polymyxin and bacitracin, and the key fatty acids of whole cell hydrolysates were iso-C15?:?0, iso-C17?:?0 and iso-C17?:?0 2-OH. The genomic DNA G+C content of the novel strain was 65.6 mol%. The 16S rRNA gene sequence showed highest similarity (97.60?%) to 'Stigmatella koreensis' strain KYC-1019 (GenBank accession no. EF112185). Phylogenetic analysis based on 16S rRNA gene sequences and MALDI-TOF MS data revealed a novel branch in the family Myxococcaceae. DNA-DNA hybridization showed only 28?% relatedness between the novel strain and the closest recognized species, Corallococcus exiguus DSM 14696(T) (97?% 16S rRNA gene sequence similarity). A recent isolate from a soil sample collected in Switzerland, MCy10622, displayed 99.9?% 16S rRNA gene sequence similarity with strain MCy1366(T) and showed almost the same characteristics. Since some morphological features like fruiting-body-like aggregates were barely reproducible in the type strain, the newly isolated strain, MCy10622, was also intensively studied. On the basis of a comprehensive taxonomic study, we propose a novel genus and species, Aggregicoccus edonensis gen. nov., sp. nov., for strains MCy1366(T) and MCy10622. The type strain of the type species is MCy1366(T) (?=?DSM 27872(T)?=?NCCB 100468(T)). PMID:24591423

Sood, Sakshi; Awal, Ram Prasad; Wink, Joachim; Mohr, Kathrin I; Rohde, Manfred; Stadler, Marc; Kämpfer, Peter; Glaeser, Stefanie P; Schumann, Peter; Garcia, Ronald; Müller, Rolf

2015-03-01

272

AN EXPLORATION OF DIVERSITY AMONG THE OSTERTAGIINAE: AFRICANASTRONGYLUS BUCEROS GEN. NOV. ET SP. NOV. (NEMATODA: TRICHOSTRONGYLOIDEA) IN AFRICAN BUFFALO (SYNCERUS CAFFER).  

Technology Transfer Automated Retrieval System (TEKTRAN)

Abomasal nematodes (Ostertagiine: Trichostrongyloidea) representing a previously unrecognized genus and species are reported in African Buffalo (Syncerus caffer caffer) from Kenya, Uganda and South Africa. Africanastrongylus buceros gen. nov. et sp. nov. is characterized by a symmetrical tapering ...

273

Hypsilara royi gen. n. and sp. n. (Coleoptera, Elmidae, Larainae) from Southern Venezuela, with a revised key to Larainae of the Western Hemisphere  

E-print Network

Here we describe a new genus, for a new species of riffle beetle, Hypsilara royi gen. n. and sp. n., from the tepui Cerro de la Neblina in southern Venezuela. This new genus can be distinguished from all other laraine ...

Maier, Crystal A.; Spangler, Paul J.

2011-07-07

274

GenBank at Los Alamos: User manual, training guide, and reference manual for the ASCII AWB  

SciTech Connect

The GenBank project at Los Alamos collects nucleotide sequence submissions from the biological research community. This work includes the processing of data received in several different forms as well as maintenance and quality control on those submissions. This manual explains the procedures involved in that work for both Los Alamos GenBank staff and off-site users. The GenBank database stores annotated DNA sequences. This manual contains the procedures for depositing these sequences into the database. There are two ways to do this. Either the sequence arrives at GenBank as a submission and is entered by the database staff or the sequence is directly entered by an off-site user. The Annotator's WorkBench (AWB), which is a database browsing and editing tool, is used in both cases. This manual is for GenBank staff and off-site users of the GenBank database at the Los Alamos National Laboratory. It contains an introduction and tutorials for AWB, as well as procedures for entering sequences either as submissions or as data directly deposited by an off-site user. Instructions for all of these are found in Chapters 2 through 4. The introduction to AWB is in Chapter 2. Instructions for submission handling are in Chapter 3. Instructions for entering sequence information are in Chapter 4. Off-site users should look at section 4.3 for instructions on entering a sequence. In addition, the manual describes various in-house curatorial tasks that are part of maintaining the database, as well as the procedures and conventions for annotating sequences. The procedures for annotation and review are in Chapters 5 and 6. The description of in-house curator's tasks is in Chapter 7. The appendices contain: Annotation conventions, two reference chapters on AWB and other utility programs, a complete list of all the forms, fields, and commands in AWB and descriptions of other (non-AWB) software utilities used by database staff.

Reese, G.C.; Keen, G.M.; Gilna, P.; Cinkosky, M.J.

1993-03-15

275

GenBank at Los Alamos: User manual, training guide, and reference manual for the OPEN LOOK[trademark] AWB  

SciTech Connect

The GenBank project at Los Alamos collects nucleotide sequence submissions from the biological research community. This work includes the processing of data received in several different forms as well as maintenance and quality control on those submissions. This manual explains the procedures involved in that work for both Los Alamos GenBank staff and off-site users. The GenBank database stores annotated DNA sequences. This manual contains the procedures for depositing these sequences into the database. There are two ways to do this. Either the sequence arrives at GenBank as a submission and is entered by the database staff or the sequence is directly entered by an off-site user. The Annotator's WorkBench (AWB), which is a database browsing and editing tool, is used in both cases. This manual is for GenBank staff and off-site users of the GenBank database at the Los Alamos National Laboratory. It contains an introduction and tutorials for AWB, as well as procedures for entering sequences either as submissions or as data directly deposited by an off-site user. Instructions for all of these are found in Chapters 2 through 4. The introduction to AWB is in Chapter 2. Instructions for submission handling are in Chapter 3. Instructions for entering sequence information are in Chapter 4. Off-site users should look at section 4.3 for instructions on entering a sequence. In addition, the manual describes various in-house curatorial tasks that are part of maintaining the database, as well as the procedures and conventions for annotating sequences. The procedures for annotation and review are in Chapters 5 and 6. The description of in-house curator's tasks is in Chapter 7. The appendices contain: Annotation conventions, two reference chapters on AWB and other utility programs, a complete list of all the forms, fields, and commands in AWB and descriptions of other (non-AWB) software utilities used by database staff.

Reese, G.C.; Keen, G.M.; Gilna, P.; Cinkosky, M.J.

1993-03-15

276

Employing NeuGen 2.0 to automatically generate realistic morphologies of hippocampal neurons and neural networks in 3D.  

PubMed

Detailed cell and network morphologies are becoming increasingly important in Computational Neuroscience. Great efforts have been undertaken to systematically record and store the anatomical data of cells. This effort is visible in databases, such as NeuroMorpho.org. In order to make use of these fast growing data within computational models of networks, it is vital to include detailed data of morphologies when generating those cell and network geometries. For this purpose we developed the Neuron Network Generator NeuGen 2.0, that is designed to include known and published anatomical data of cells and to automatically generate large networks of neurons. It offers export functionality to classic simulators, such as the NEURON Simulator by Hines and Carnevale (2003). NeuGen 2.0 is designed in a modular way, so any new and available data can be included into NeuGen 2.0. Also, new brain areas and cell types can be defined with the possibility of constructing user-defined cell types and networks. Therefore, NeuGen 2.0 is a software package that grows with each new piece of anatomical data, which subsequently will continue to increase the morphological detail of automatically generated networks. In this paper we introduce NeuGen 2.0 and apply its functionalities to the CA1 hippocampus. Runtime and memory benchmarks show that NeuGen 2.0 is applicable to generating very large networks, with high morphological detail. PMID:23111491

Wolf, S; Grein, S; Queisser, G

2013-04-01

277

Phenotype-Genotype Integrator (PheGenI): synthesizing genome-wide association study (GWAS) data with existing genomic resources.  

PubMed

Rapidly accumulating data from genome-wide association studies (GWASs) and other large-scale studies are most useful when synthesized with existing databases. To address this opportunity, we developed the Phenotype-Genotype Integrator (PheGenI), a user-friendly web interface that integrates various National Center for Biotechnology Information (NCBI) genomic databases with association data from the National Human Genome Research Institute GWAS Catalog and supports downloads of search results. Here, we describe the rationale for and development of this resource. Integrating over 66,000 association records with extensive single nucleotide polymorphism (SNP), gene, and expression quantitative trait loci data already available from the NCBI, PheGenI enables deeper investigation and interrogation of SNPs associated with a wide range of traits, facilitating the examination of the relationships between genetic variation and human diseases. PMID:23695286

Ramos, Erin M; Hoffman, Douglas; Junkins, Heather A; Maglott, Donna; Phan, Lon; Sherry, Stephen T; Feolo, Mike; Hindorff, Lucia A

2014-01-01

278

Phenotype–Genotype Integrator (PheGenI): synthesizing genome-wide association study (GWAS) data with existing genomic resources  

PubMed Central

Rapidly accumulating data from genome-wide association studies (GWASs) and other large-scale studies are most useful when synthesized with existing databases. To address this opportunity, we developed the Phenotype–Genotype Integrator (PheGenI), a user-friendly web interface that integrates various National Center for Biotechnology Information (NCBI) genomic databases with association data from the National Human Genome Research Institute GWAS Catalog and supports downloads of search results. Here, we describe the rationale for and development of this resource. Integrating over 66?000 association records with extensive single nucleotide polymorphism (SNP), gene, and expression quantitative trait loci data already available from the NCBI, PheGenI enables deeper investigation and interrogation of SNPs associated with a wide range of traits, facilitating the examination of the relationships between genetic variation and human diseases. PMID:23695286

Ramos, Erin M; Hoffman, Douglas; Junkins, Heather A; Maglott, Donna; Phan, Lon; Sherry, Stephen T; Feolo, Mike; Hindorff, Lucia A

2014-01-01

279

The impact of message appeal and message source on Gen Y consumers' attitudes and purchase intentions toward American Apparel  

Microsoft Academic Search

A 3 × 2 experimental design was used to examine the impact of message appeal (fair labor, sex, or a combination of fair labor and sex) and message source (company advertisements or third-party publicity) upon Gen Y consumers' (N = 258) intent to patronize the retailer, American Apparel. Results supported models of hierarchical effects of advertising on patronage intention as well as the inclusion

Ruoh-Nan Yan; Jennifer Paff Ogle; Karen H. Hyllegard

2010-01-01

280

J. Phys. A: Math. Gen. 17 (1984) L895-L898. Printed in Great Britain LETTER TO THE EDITOR  

E-print Network

J. Phys. A: Math. Gen. 17 (1984) L895-L898. Printed in Great Britain LETTER TO THE EDITOR and Astronomy, Tel-Aviv University, Tel-Aviv, Israel Received 6 July 1984 Abstract. DC conductivity-4470/84/160895 +04s02.25 @ 1984 The Institute of Physics L895 #12;L896 Letter to the Editor 1984) and 1.31 (Adler

Palevski, Alexander

281

Surface Map Traffic Intent Displays and Net-Centric Data-link Communications for NextGen  

NASA Technical Reports Server (NTRS)

By 2025, U.S. air traffic is predicted to increase three fold and may strain the current air traffic management system, which may not be able to accommodate this growth. In response to this challenge, a revolutionary new concept has been proposed for U.S. aviation operations, termed the Next Generation Air Transportation System or "NextGen". Many key capabilities are being identified to enable NextGen, including the use of data-link communications. Because NextGen represents a radically different approach to air traffic management and requires a dramatic shift in the tasks, roles, and responsibilities for the flight deck, there are numerous research issues and challenges that must be overcome to ensure a safe, sustainable air transportation system. Flight deck display and crew-vehicle interaction concepts are being developed that proactively investigate and overcome potential technology and safety barriers that might otherwise constrain the full realization of NextGen. The paper describes simulation research, conducted at National Aeronautics and Space Administration (NASA) Langley Research Center, examining data-link communications and traffic intent data during envisioned four-dimensional trajectory (4DT)-based and equivalent visual (EV) surface operations. Overall, the results suggest that controller pilot data-link communications (CPDLC) with the use of mandatory pilot read-back of all clearances significantly enhanced situation awareness for 4DT and EV surface operations. The depiction of graphical traffic state and intent information on the surface map display further enhanced off-nominal detection and pilot qualitative reports of safety and awareness.

Shelton, Kevin J.; Prinzel, Lawrence J., III; Jones, Denise R.; Allamandola, Angela S.; Arthur, Jarvis J., III; Bailey, Randall E.

2009-01-01

282

Hammondia hammondi gen. nov., sp.nov., from domestic cats, a new coccidian related to Toxoplasma and Sarcocystis  

Microsoft Academic Search

Summary Hammondia hammondi gen.nov.,sp.nov. (Eimeriorina:Sarcocystidae) is described as an obligate heteroxenous protozoon of domestic cats (final host) and laboratory mice (experimental intermediate host). Oocysts from the final host are infectious only for the intermediate host; and cysts from the intermediate host are infectious only for the final host. Intracellular cysts develop principally in striated muscle of mice that ingest oocysts,

J. K. Frenkel; J. P. Dubey

1975-01-01

283

http://www.u-tokyo.ac.jp/fin03/pdf/H21.1.26torihiki.pdf http://www.u-tokyo.ac.jp/gen01/reiki_int/reiki_kaikei/zaimukaikeiininkitei.pdf  

E-print Network

1 2 3 4 5 http://www.u-tokyo.ac.jp/fin03/pdf/H21.1.26torihiki.pdf http://www.u-tokyo.ac.jp/gen01/reiki_int/reiki_kaikei/zaimukaikeiininkitei.pdf http://www.u-tokyo.ac.jp/gen01/reiki_int/reiki_kaikei/keiyakujimutoriatukaikite.pdf http://www.u-tokyo.ac.jp/gen01/reiki

Miyashita, Yasushi

284

A rapid UPLC method for simultaneous determination of eleven components in ‘Ge-Gen-Qin-Lian’ decoction  

PubMed Central

Background: ‘Ge-Gen-Qin-Lian’ Decoction derived from ‘Shang-Han-Lun’ compiled by Zhang Zhongjing. It is widely used in the treatment of acute gastroenteritis, bacillary dysentery, virus diarrhea. This paper describes a sensitive and specific assay for the determination of the 11-marker compounds using ultra performance liquid chromatography (UPLC). Objective: To develop an UPLC method for simultaneous determination of 11 bioactive compounds in ‘Ge-Gen-Qin-Lian’ preparations. Materials and Methods: The chromatography analysis was performed on an Agilent Proshell 120 EC-C18 column (4.6 × 50 mm, 2.7 ?m) at 30°C with a gradient elution of methanol, 0.5% formic acid and 0.5% ammonium acetate at a flow rate 1.0 ml/min and UV detected at 270 nm. Results: All calibration curves showed good linear regression (r ? 0.9993) within tested ranges. Limits of detection (LOD) and limits of quantification (LOQ) fell in the range between 0.0691-1.04 ?g/ml and 0.23–3.43 ?g/ml, respectively. The mean recovery of each herbal medicine ranged from 96.60 to 102.11%. Conclusion: The method was validated for repeatability, precision, stability, accuracy, and selectivity. The validated method was successfully applied to simultaneous analysis of these active components in ‘Ge-Gen-Qin-Lian’ decoction. PMID:25422547

An, Rui; You, Lisha; Zhang, Yizhu; Wang, Xinhong; Ma, Yuemin

2014-01-01

285

Description of Tersicoccus phoenicis gen. nov., sp. nov. isolated from spacecraft assembly clean room environments.  

PubMed

Two strains of aerobic, non-motile, Gram-reaction-positive cocci were independently isolated from geographically distinct spacecraft assembly clean room facilities (Kennedy Space Center, Florida, USA and Centre Spatial Guyanais, Kourou, French Guiana). A polyphasic study was carried out to delineate the taxonomic identity of these two isolates (1P05MA(T) and KO_PS43). The 16S rRNA gene sequences exhibited a high similarity when compared to each other (100 %) and lower than 96.7 % relatedness with Arthrobacter crystallopoietes ATCC 15481(T), Arthrobacter luteolus ATCC BAA-272(T), Arthrobacter tumbae DSM 16406(T) and Arthrobacter subterraneus DSM 17585(T). In contrast with previously described Arthrobacter species, the novel isolates maintained their coccidal morphology throughout their growth and did not exhibit the rod-coccus life cycle typically observed in nearly all Arthrobacter species, except A. agilis. The distinct taxonomic identity of the novel isolates was confirmed based on their unique cell-wall peptidoglycan type (A.11.20; Lys-Ser-Ala2) and polar lipid profile (presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and two unknown glycolipids). The G+C content of the genomic DNA was 70.6 mol%. The novel strains revealed MK-9(H2) and MK-8(H2) as dominant menaquinones and exhibited fatty acid profiles consisting of major amounts of anteiso-C15 : 0 and anteiso-C17 : 0 and moderate amounts of iso-C15 : 0 discriminating them again from closely related Arthrobacter species. Based on these observations, the authors propose that strains 1P05MA(T) and KO_PS43 be assigned into a separate genus Tersicoccus gen. nov. For this new taxon, comprising strains 1P05MA(T) and KO_PS43, we propose the name Tersicoccus phoenicis gen. nov., sp. nov. (the type species of Tersicoccus), represented by the type strain Tersicoccus phoenicis 1P05MA(T) ( = NRRL B-59547(T) = DSM 30849(T)). PMID:23223813

Vaishampayan, Parag; Moissl-Eichinger, Christine; Pukall, Rüdiger; Schumann, Peter; Spröer, Cathrin; Augustus, Angela; Roberts, Anne Hayden; Namba, Greg; Cisneros, Jessica; Salmassi, Tina; Venkateswaran, Kasthuri

2013-07-01

286

GenInfoGuard—A Robust and Distortion-Free Watermarking Technique for Genetic Data  

PubMed Central

Genetic data, in digital format, is used in different biological phenomena such as DNA translation, mRNA transcription and protein synthesis. The accuracy of these biological phenomena depend on genetic codes and all subsequent processes. To computerize the biological procedures, different domain experts are provided with the authorized access of the genetic codes; as a consequence, the ownership protection of such data is inevitable. For this purpose, watermarks serve as the proof of ownership of data. While protecting data, embedded hidden messages (watermarks) influence the genetic data; therefore, the accurate execution of the relevant processes and the overall result becomes questionable. Most of the DNA based watermarking techniques modify the genetic data and are therefore vulnerable to information loss. Distortion-free techniques make sure that no modifications occur during watermarking; however, they are fragile to malicious attacks and therefore cannot be used for ownership protection (particularly, in presence of a threat model). Therefore, there is a need for a technique that must be robust and should also prevent unwanted modifications. In this spirit, a watermarking technique with aforementioned characteristics has been proposed in this paper. The proposed technique makes sure that: (i) the ownership rights are protected by means of a robust watermark; and (ii) the integrity of genetic data is preserved. The proposed technique—GenInfoGuard—ensures its robustness through the “watermark encoding” in permuted values, and exhibits high decoding accuracy against various malicious attacks. PMID:25689741

Khan, Sharifullah; Anwar, Zahid; Kamran, Muhammad

2015-01-01

287

Ab Initio Studies of SimGen (m+n <= 5) Nanoclusters  

NASA Astrophysics Data System (ADS)

Electronic and geometric structure properties of SimGen(m+n <= 5) nanoclusters have been investigated using hybrid density functional B3LYP, 6-311G (3df, 3pd) basis set, and the GAUSSIAN 03 software. For the Si atom, the computed values of the ionization potential and electron affinity are 8.11 and 1.10eV, and for the Ge atom, the values are 7.90 and 1.14eV. The experimental values are 8.15, 1.39, 7.90, and 1.23eV, respectively. Various possible geometries have been spin-optimized to determine the global minimum for each nanocluster. We will present the electronic and geometric structures of the isomers of each nanocluster, including bond length, symmetry group, electronic state, binding energy, HOMO-LUMO gap, ionization potential, and electron affinity. In addition, the harmonic frequencies, fragmentation energies, average coordination number and Mulliken atomic charges will also be discussed for the ground states of the nanoclusters.

Duesman, Sarah; Ray, Asok

2011-03-01

288

Probing genomic diversity and evolution of Streptococcus suis serotype 2 by NimbleGen tiling arrays  

PubMed Central

Background Our previous studies revealed that a new disease form of streptococcal toxic shock syndrome (STSS) is associated with specific Streptococcus suis serotype 2 (SS2) strains. To achieve a better understanding of the pathogenicity and evolution of SS2 at the whole-genome level, comparative genomic analysis of 18 SS2 strains, selected on the basis of virulence and geographic origin, was performed using NimbleGen tiling arrays. Results Our results demonstrate that SS2 isolates have highly divergent genomes. The 89K pathogenicity island (PAI), which has been previously recognized as unique to the Chinese epidemic strains causing STSS, was partially included in some other virulent and avirulent strains. The ABC-type transport systems, encoded by 89K, were hypothesized to greatly contribute to the catastrophic features of STSS. Moreover, we identified many polymorphisms in genes encoding candidate or known virulence factors, such as PlcR, lipase, sortases, the pilus-associated proteins, and the response regulator RevS and CtsR. On the basis of analysis of regions of differences (RDs) across the entire genome for the 18 selected SS2 strains, a model of microevolution for these strains is proposed, which provides clues into Streptococcus pathogenicity and evolution. Conclusions Our deep comparative genomic analysis of the 89K PAI present in the genome of SS2 strains revealed details into how some virulent strains acquired genes that may contribute to STSS, which may lead to better environmental monitoring of epidemic SS2 strains. PMID:21554741

2011-01-01

289

Kretzoiarctos gen. nov., the oldest member of the giant panda clade.  

PubMed

The phylogenetic position of the giant panda, Ailuropoda melanoleuca (Carnivora: Ursidae: Ailuropodinae), has been one of the most hotly debated topics by mammalian biologists and paleontologists during the last century. Based on molecular data, it is currently recognized as a true ursid, sister-taxon of the remaining extant bears, from which it would have diverged by the Early Miocene. However, from a paleobiogeographic and chronological perspective, the origin of the giant panda lineage has remained elusive due to the scarcity of the available Miocene fossil record. Until recently, the genus Ailurarctos from the Late Miocene of China (ca. 8-7 mya) was recognized as the oldest undoubted member of the Ailuropodinae, suggesting that the panda lineage might have originated from an Ursavus ancestor. The role of the purported ailuropodine Agriarctos, from the Miocene of Europe, in the origins of this clade has been generally dismissed due to the paucity of the available material. Here, we describe a new ailuropodine genus, Kretzoiarctos gen. nov., based on remains from two Middle Miocene (ca. 12-11 Ma) Spanish localities. A cladistic analysis of fossil and extant members of the Ursoidea confirms the inclusion of the new genus into the Ailuropodinae. Moreover, Kretzoiarctos precedes in time the previously-known, Late Miocene members of the giant panda clade from Eurasia (Agriarctos and Ailurarctos). The former can be therefore considered the oldest recorded member of the giant panda lineage, which has significant implications for understanding the origins of this clade from a paleobiogeographic viewpoint. PMID:23155439

Abella, Juan; Alba, David M; Robles, Josep M; Valenciano, Alberto; Rotgers, Cheyenn; Carmona, Raül; Montoya, Plinio; Morales, Jorge

2012-01-01

290

Katatopygia gen. n., a monophyletic branch segregated from Boletina (Diptera, Mycetophilidae)  

PubMed Central

Abstract The genus Katatopygia gen. n. is proposed for the Boletina erythropyga/punctus-group that was first introduced by Garrett (1924, 1925) and currently comprises eight described species. Molecular studies have strongly indicated that this group forms a monophyletic sister-group to a clade consisting of all other Boletina, Coelosia and Gnoriste, and its monophyly is supported by morphological data as well. The new genus includes the following species: Katatopygia antoma (Garrett, 1924), comb. n., Katatopygia antica (Garrett, 1924), comb. n., Katatopygia erythropyga (Holmgren, 1883), comb. n.,Katatopygia hissarica (Zaitzev & Polevoi, 2002), comb. n., Katatopygia magna (Garrett, 1925), comb. n., Katatopygia laticauda (Saigusa, 1968), comb. n., Katatopygia neoerythropyga (Zaitzev & Polevoi, 2002), comb. n. andKatatopygia sahlbergi (Lundström, 1906), comb. n., all transferred from Boletina. Katatopygia sahlbergi is found to be a senior synonym of Boletina punctus Garrett, 1925, syn. n. A phylogeny based on morphological data and using parsimony analysis yielded four most parsimonious trees where the new genus is retrieved as monophyletic with high support. Katatopygia neoerythropyga is found to be the sister-taxon to all other species that form two clades, one with Katatopygia sahlbergi-like species and one with Katatopygia erythropyga-like species. A key to males of Katatopygia is provided. PMID:22451790

Martinsson, Svante; Kjærandsen, Jostein

2012-01-01

291

Applicability of the Ge(n,?) Reaction for Estimating Thermal Neutron Flux  

NASA Astrophysics Data System (ADS)

A simple experimental setup was used to measure gamma lines appearing in spectra after interactions of neutrons with Ge in the active volume of a high-purity germanium detector placed in a low-background shield. As source of neutrons a 252Cf spontaneous fission source and different thicknesses of PVC plates were used to slow down neutrons. A cadmiumenvelope was placed over the detector dipstick to identify the effect from slow and fast neutrons. Intensities of several characteristic ?-lines were measured, including intensity of the 139.9 keV ?-line from the reaction 74Ge(n,?)75mGe, usually used for estimation of thermal neutron flux. Obtained results signify that only a part of the detected 139.9 keV ?-rays originate from thermal neutron capture. Some preliminary results indicate that in our detection setup thermal neutron capture contributes with 30% to 50% to the total intensity of the 139.9 keV ?-line, depending on the thickness of the PVC plates.

Nikolov, J.; Medi?, Ž.; Jovan?evi?, N.; Hansman, J.; Todorovi?, N.; Krmar, M.

292

Spelaeicoccus albus gen. nov., sp. nov., an actinobacterium isolated from a natural cave.  

PubMed

A novel Gram-stain-positive, non-endospore-forming, coccoid actinobacterium, designated strain D3-40(T), was isolated from the soil of a natural cave and characterized by means of a polyphasic taxonomic analysis. 16S rRNA gene sequence analysis showed that strain D3-40(T) is a member of the suborder Micrococcineae and forms a distinct branch at the base of a Brevibacteriaceae cluster. Its closest relative is the type strain of Brevibacterium samyangense (95.7?% sequence similarity). The chemotaxonomic characteristics were as follows: the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid; the major menaquinone was MK-9(H2); the polar lipids consisted of phosphatidylglycerol, phosphatidylinositol, an unknown glycolipid and an unknown phospholipid; the major fatty acids were anteiso-C15?:?0, anteiso-C17?:?0, iso-C15?:?0, C16?:?0 and cyclohexyl-C17?:?0; mycolic acids were absent. The G+C content of the DNA was 64.3 mol%. On the basis of morphological, chemotaxonomic and phylogenetic data, it is suggested that the organism represents a novel species of a new genus within the family Brevibacteriaceae, for which the name Spelaeicoccus albus gen. nov., sp. nov. is proposed. The type strain of the type species is D3-40(T) (?=?KCTC 29141(T)?=?DSM 26341(T)). PMID:23811133

Lee, Soon Dong

2013-11-01

293

Mageeibacillus indolicus gen. nov., sp. nov.: A novel bacterium isolated from the female genital tract.  

PubMed

Three isolates of a bacterium recovered from human endometrium using conventional culture methods were characterized biochemically and subjected to 16S rRNA gene sequencing and phylogenetic analysis. Isolates were non-motile, obligately anaerobic, non-spore forming, asaccharolytic, non-cellulolytic, indole positive, Gram positive rods. Cell wall fatty acid profiling revealed C14:0, C16:0, C18:2 ?6, 9c, C18:1 ?9c and C18:0 to be the major fatty acid composition. The DNA mol % G+C was determined to be 44.2%. 16S rRNA gene sequence analysis revealed only 91% sequence similarity with the closest cultivated bacterial isolate, Saccharofermentans acetigenes. Based on genotypic and phenotypic data, all three isolates are considered to be members of the same species and data suggest it represents a novel genus and species in the order Clostridiales with an association with Clostridium rRNA cluster III within the family Ruminococcaceae. We propose the name, Mageeibacillus indolicus gen. nov., sp. nov. The type strain is BAA-2120(T) and CCUG 59143(T). PMID:25482717

Austin, Michele N; Rabe, Lorna K; Srinivasan, Sujatha; Fredricks, David N; Wiesenfeld, Harold C; Hillier, Sharon L

2015-04-01

294

Salisediminibacterium halotolerans gen. nov., sp. nov., a halophilic bacterium from soda lake sediment.  

PubMed

An orange-pigmented, Gram-reaction-positive, non-spore-forming, halophilic, alkali-tolerant rod, designated strain halo-2(T), was isolated from sediment of Xiarinaoer soda lake, in China's Inner Mongolia Autonomous Region. Strain halo-2(T) grew in a complex medium with 3-30 % (w/v) NaCl and at pH 5-10. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was MK-7. The predominant cellular fatty acids were anteiso-C(15 : 0) (43.6 %), anteiso-C(17 : 0) (14.8 %) and iso-C(15 : 0) (6.8 %) and the polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA G+C content of the novel strain was 48.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain halo-2(T) was most closely related to Bacillus agaradhaerens DSM 8721(T) (93.9 % sequence similarity). However, strain halo-2(T) could be clearly differentiated from its closest phylogenetic relatives on the basis of several phenotypic, genotypic and chemotaxonomic characteristics. Strain halo-2(T) therefore represents a novel species in a new genus for which the name Salisediminibacterium halotolerans gen. nov., sp. nov. is proposed. The type strain of the type species is halo-2(T) (= CGMCC 1.7654(T) = NBRC 104935(T)). PMID:22039006

Jiang, Feng; Cao, Shu-Juan; Li, Zhao-Hu; Fan, Hua; Li, Hai-Feng; Liu, Wei-Jie; Yuan, Hong-Li

2012-09-01

295

Zhihengliuella halotolerans gen. nov., sp. nov., a novel member of the family Micrococcaceae.  

PubMed

The actinobacterial strain YIM 70185(T) was isolated from a saline soil sample collected from Qinghai province, north-west China, and subjected to a taxonomic investigation. Phylogenetic analysis based on 16S rRNA gene sequences revealed 93.5-96.4 % similarity to members of related genera in the family Micrococcaceae. In the phylogenetic dendrogram based on 16S rRNA gene sequence analysis, strain YIM 70185(T) formed a separate clade next to the genera Micrococcus and Citricoccus within the family Micrococcaceae. The peptidoglycan type was A4alpha, l-lys-l-ala-l-Glu. Cell-wall sugars contained glucose and tyvelose. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and an unknown glycolipid. The menaquinones were MK-9, MK-10 and MK-8 (molar ratio 5 : 2 : 1). The major fatty acids were ai-C(15 : 0) and i-C(15 : 0) and the DNA G+C content was 66.5 mol%. These chemotaxonomic profiles supported the assignment of strain YIM 70185(T) to a novel genus within the family Micrococcaceae. The name Zhihengliuella halotolerans gen. nov., sp. nov. is proposed. The type strain of Zhihengliuella halotolerans is YIM 70185(T) (=DSM 17364(T)=KCTC 19085(T)). PMID:17473251

Zhang, Yu-Qin; Schumann, Peter; Yu, Li-Yan; Liu, Hong-Yu; Zhang, Yue-Qin; Xu, Li-Hua; Stackebrandt, Erko; Jiang, Cheng-Lin; Li, Wen-Jun

2007-05-01

296

Isolation and classification of a novel marine Bacteroidetes as Frondibacter aureus gen. nov., sp. nov.  

PubMed

A facultatively anaerobic, Gram-stain negative, golden-yellow pigmented, non-motile and rod-shaped bacterium, designated strain A5Q-67(T) was isolated from leaf litter collected at the mangrove estuary of Nakama River, Japan. Phylogenetic analysis based on the 16S rRNA gene sequence revealed the novel isolate was affiliated with the family Flavobacteriaceae of the phylum Bacteroidetes and that it showed highest sequence similarity (94.2 %) to Imtechella halotolerans K1(T). The strain could be differentiated phenotypically from recognized members of the family Flavobacteriaceae. The major fatty acids of strain A5Q-67(T) were identified as iso-C17:0 3-OH, summed feature 1 (iso-C15:1 H and/or C13:0 3-OH) and iso-C15:0 as defined by the MIDI system. The DNA G+C content was determined to be 36.7 mol%, the major respiratory quinone was identified as menaquinone 6 (MK-6) and a polar lipid profile was present consisting of phosphatidylethanolamine, two unidentified aminolipids and an unidentified lipid. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel genus for which the name Frondibacter aureus gen. nov., sp. nov. is proposed. The type strain of F. aureus is A5Q-67(T) (=KCTC 32991(T) = NBRC 110021(T)). PMID:25385000

Yoon, Jaewoo; Adachi, Kyoko; Kasai, Hiroaki

2015-02-01

297

Leisingera methylohalidivorans gen. nov., sp. nov., a marine methylotroph that grows on methyl bromide  

USGS Publications Warehouse

A marine methylotroph, designated strain MB2T, was isolated for its ability to grow on methyl bromide as a sole carbon and energy source. Methyl chloride and methyl iodide also supported growth, as did methionine and glycine betaine. A limited amount of growth was observed with dimethyl sulfide. Growth was also noted with unidentified components of the complex media marine broth 2216, yeast extract and Casamino acids. No growth was observed on methylated amines, methanol, formate, acetate, glucose or a variety of other substrates. Growth on methyl bromide and methyl iodide resulted in their oxidation to CO2 with stoichiometric release of bromide and iodide, respectively. Strain MB2T exhibited growth optima at NaCl and Mg2+ concentrations similar to that of seawater. Phylogenetic analysis of the 16S rDNA sequence placed this strain in the ??-Proteobacteria in proximity to the genera Ruegeria and Roseobacter. It is proposed that strain MB2T (= ATCC BAA-92T = DSM 14336T) be designated Leisingera methylohalidivorans gen. nov., sp. nov.

Schaefer, J.K.; Goodwin, K.D.; McDonald, I.R.; Murrell, J.C.; Oremland, R.S.

2002-01-01

298

Emergency Decay Heat Removal in a GEN-IV Gas-Cooled Fast Reactor  

SciTech Connect

A series of transient analyses using the system code RELAP5-3d has been performed to confirm the efficacy of a proposed hybrid active/passive combination approach to the decay heat removal for an advanced 2400 MWt GEN-IV gas-cooled fast reactor. The accident sequence of interest is a station blackout simultaneous with a small break (10 sq.inch/0.645 m{sup 2}) in the reactor vessel. The analyses cover the three phases of decay heat removal in a depressurization accident: (1) forced flow cooling by the power conversion unit (PCU) coast down, (2) active forced flow cooling by a battery powered blower, and (3) passive cooling by natural circulation. The blower is part of an emergency cooling system (ECS) that by design is to sustain passive decay heat removal via natural circulation cooling 24 hours after shutdown. The RELAP5 model includes the helium-cooled reactor, the ECS (primary and secondary side), the PCU with all the rotating machinery (turbine and compressors) and the heat transfer components (recuperator, pre-cooler and inter-cooler), and the guard containment that surrounds the reactor and the PCU. The transient analysis has demonstrated the effectiveness of passive decay heat removal by natural circulation cooling when the guard containment pressure is maintained at or above 800 kPa. (authors)

Cheng, Lap Y.; Ludewig, Hans; Jo, Jae [Brookhaven National Laboratory, P.O. Box 5000, Upton, NY 11973-5000 (United States)

2006-07-01

299

Tepidicella xavieri gen. nov., sp. nov., a betaproteobacterium isolated from a hot spring runoff.  

PubMed

Strains TU-16T and TU-18, two non-pigmented bacterial isolates with an optimum growth temperature of about 45 degrees C and an optimum pH of about 8.5-9.0, were recovered from the Furnas geothermal area on the Island of São Miguel in the Azores. Phylogenetic analysis of the 16S rRNA gene sequence of these strains indicated that they represent a novel species in a new genus of the phylum Betaproteobacteria. The major fatty acids of strains TU-16T and TU-18 were 16 : 0 and 18 : 1omega7c. Ubiquinone 8 was the major respiratory quinone and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The novel isolates were aerobic; thiosulfate was oxidized to sulfate in the presence of a metabolizable carbon source. The organism assimilated organic acids and amino acids, but did not assimilate carbohydrates or polyols. Based on phylogenetic analyses and physiological and biochemical characteristics, it is proposed that strain TU-16T (=LMG 23030T = CIP 108724T) represents the type strain of a novel species in a new genus, Tepidicella xavieri gen. nov., sp. nov. PMID:16585714

França, Luis; Rainey, Fred A; Nobre, M Fernanda; da Costa, Milton S

2006-04-01

300

Flavihumibacter petaseus gen. nov., sp. nov., isolated from soil of a subtropical rainforest.  

PubMed

A yellow-coloured bacterium, T41(T), was isolated from a soil sample of a subtropical rainforest in Nepal. Cells were Gram-reaction-positive, aerobic, non-motile, short rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain formed a cluster with Terrimonas ferruginea, Terrimonas lutea, Niabella soli, Flavisolibacter ginsengiterrae, Flavisolibacter ginsengisoli, Niastella yeongjuensis and Niastella koreensis in the phylum Bacteroidetes. The strain showed the highest sequence similarity to the type strain of Terrimonas lutea (93.2 %). The major isoprenoid quinone was MK-7 and the predominant cellular fatty acids (>10 %) were iso-15 : 0 (33.8 %), iso-15 : 1 G (13.3 %) and iso-17 : 0 3-OH (12.9 %). The DNA G+C content was 48.1 mol%. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, strain T41(T) represents a novel species in a new genus in the phylum Bacteroidetes, for which the name Flavihumibacter petaseus gen. nov., sp. nov. is proposed. The type strain of Flavihumibacter petaseus is strain T41(T) (=CGMCC 1.7723(T) =NBRC 106054(T)). PMID:19700449

Zhang, Nan Nan; Qu, Jian Hang; Yuan, Hong Li; Sun, Yan Mei; Yang, Jin Shui

2010-07-01

301

Liberibacter crescens gen. nov., sp. nov., the first cultured member of the genus Liberibacter.  

PubMed

The Gram-stain-negative, rod-shaped bacterial isolate BT-1(T) is the closest relative to the genus 'Candidatus Liberibacter' cultured to date. BT-1(T) was recovered from the phloem sap of a defoliating mountain papaya in Puerto Rico. The BT-1(T) 16S rRNA gene sequence showed that strain BT-1(T) is most closely related to members of the genus 'Ca. Liberibacter' sharing 94.7% 16S rRNA gene sequence similarity with 'Ca. Liberibacter americanus' and 'Ca. Liberibacter asiaticus'. Additionally, average nucleotide identity, 16S rRNA gene sequences and conserved protein sequences supported inclusion of the previously described species of the genus 'Ca. Liberibacter' in a genus with BT-1(T). The prominent fatty acids of isolate BT-1(T) were C18 : 1?7c (77.2%), C16 : 0 OH (4.8%), C18 : 0 (4.4%) and C16 : 0 (3.5%). Both physiological and genomic characteristics support the creation of the genus Liberibacter, as well as the novel species Liberibacter crescens gen. nov., sp. nov. with type strain BT-1(T) (?= ATCC BAA-2481(T)?= DSM 26877(T)). PMID:24786353

Fagen, Jennie R; Leonard, Michael T; Coyle, Janelle F; McCullough, Connor M; Davis-Richardson, Austin G; Davis, Michael J; Triplett, Eric W

2014-07-01

302

Lombardia GENS: a collaborative registry for monogenic diseases associated with stroke  

PubMed Central

Summary The Italian region of Lombardy, with its existing stroke centers and high-technology laboratories, provides a favorable context for studying monogenic diseases associated with stroke. The Lombardia GENS project was set up to create a regional network for the diagnosis of six monogenic diseases associated with stroke: CADASIL, Fabry disease, MELAS, familial and sporadic hemiplegic migraine, hereditary cerebral amyloid angiopathy and Marfan syndrome. The network comprises 36 stroke centers and seven high-technology laboratories, performing molecular analysis. In this context, all stroke/TIA patients fulfilling clinical criteria for monogenic diseases are currently being included in an ongoing study. Demographic, clinical and family data and diagnostic criteria are collected using standardized forms. On the basis of stroke incidence in Lombardy and the reported prevalence of the diseases considered, we expect, during the course of the study, to collect datasets and DNA samples from more than 200 stroke patients suspected of having monogenic diseases. This will allow evaluation of the regional burden and better phenotype characterization of monogenic diseases associated with stroke. PMID:23158583

Bersano, Anna; Baron, Pierluigi; Lanfranconi, Silvia; Trobia, Nadia; Sterzi, Roberto; Motto, Cristina; Comi, Giancarlo; Sessa, Maria; Martinelli-Boneschi, Filippo; Micieli, Giuseppe; Ferrarese, Carlo; Santoro, Patrizia; Parati, Eugenio; Boncoraglio, Giorgio; Padovani, Alessandro; Pezzini, Alessandro; Candelise, Livia

2012-01-01

303

Synthetic and Enhanced Vision Systems for NextGen (SEVS) Simulation and Flight Test Performance Evaluation  

NASA Technical Reports Server (NTRS)

The Synthetic and Enhanced Vision Systems for NextGen (SEVS) simulation and flight tests are jointly sponsored by NASA's Aviation Safety Program, Vehicle Systems Safety Technology project and the Federal Aviation Administration (FAA). The flight tests were conducted by a team of Honeywell, Gulfstream Aerospace Corporation and NASA personnel with the goal of obtaining pilot-in-the-loop test data for flight validation, verification, and demonstration of selected SEVS operational and system-level performance capabilities. Nine test flights (38 flight hours) were conducted over the summer and fall of 2011. The evaluations were flown in Gulfstream.s G450 flight test aircraft outfitted with the SEVS technology under very low visibility instrument meteorological conditions. Evaluation pilots flew 108 approaches in low visibility weather conditions (600 ft to 2400 ft visibility) into various airports from Louisiana to Maine. In-situ flight performance and subjective workload and acceptability data were collected in collaboration with ground simulation studies at LaRC.s Research Flight Deck simulator.

Shelton, Kevin J.; Kramer, Lynda J.; Ellis,Kyle K.; Rehfeld, Sherri A.

2012-01-01

304

Development of Complexity Science and Technology Tools for NextGen Airspace Research and Applications  

NASA Technical Reports Server (NTRS)

The objective of this research by NextGen AeroSciences, LLC is twofold: 1) to deliver an initial "toolbox" of algorithms, agent-based structures, and method descriptions for introducing trajectory agency as a methodology for simulating and analyzing airspace states, including bulk properties of large numbers of heterogeneous 4D aircraft trajectories in a test airspace -- while maintaining or increasing system safety; and 2) to use these tools in a test airspace to identify possible phase transition structure to predict when an airspace will approach the limits of its capacity. These 4D trajectories continuously replan their paths in the presence of noise and uncertainty while optimizing performance measures and performing conflict detection and resolution. In this approach, trajectories are represented as extended objects endowed with pseudopotential, maintaining time and fuel-efficient paths by bending just enough to accommodate separation while remaining inside of performance envelopes. This trajectory-centric approach differs from previous aircraft-centric distributed approaches to deconfliction. The results of this project are the following: 1) we delivered a toolbox of algorithms, agent-based structures and method descriptions as pseudocode; and 2) we corroborated the existence of phase transition structure in simulation with the addition of "early warning" detected prior to "full" airspace. This research suggests that airspace "fullness" can be anticipated and remedied before the airspace becomes unsafe.

Holmes, Bruce J.; Sawhill, Bruce K.; Herriot, James; Seehart, Ken; Zellweger, Dres; Shay, Rick

2012-01-01

305

Cosmosperma polyloba gen. et sp. nov., a seed plant from the Upper Devonian of South China  

NASA Astrophysics Data System (ADS)

Seed plants with ovules were abundant in the Late Devonian of Euramerica and they contribute significantly to our understanding of their early history. However, coeval ovules have been scarce in other regions of the world. Specimens of the seed plant Cosmosperma polyloba gen. et sp. nov. Wang et al. were recently obtained from the Upper Devonian (Famennian) Wutong Formation, at Fanwan Village, Changxing County, Zhejiang Province, China. This new seed plant has cupulate ovules, the uniovulate cupules with up to 16 distal segments and with minute spines on the outer surface, synangiate pollen organs bearing six to eight microsporangia fused only at the base, and planate and highly dissected pinnules in alternate arrangement. It differs from other Devonian seed plants mainly in the organization and position of the uniovulate and ornamented cupule, and in the highly dissected pinnules. Cosmosperma Wang et al. represents the first Devonian ovules recovered from China or eastern Asia and further illustrates the diversity of early spermatophytes. As for the Late Devonian seed plants, it is suggested that the pollen organs are synangiate and simple in organization, and the branches and leaves are generally planate.

Wang, Deming; Liu, Le; Meng, Meicen; Xue, Jinzhuang; Liu, Tuo; Guo, Yun

2014-08-01

306

Using Game Theoretic Models to Predict Pilot Behavior in NextGen Merging and Landing Scenario  

NASA Technical Reports Server (NTRS)

In this paper, we present an implementation of the Semi Network-Form Game framework to predict pilot behavior in a merging and landing scenario. In this scenario, two aircraft are approaching to a freeze horizon with approximately equal distance when they become aware of each other via an ADS-B communication link that will be available in NextGen airspace. Both pilots want to gain advantage over the other by entering the freeze horizon earlier and obtain the first place in landing. They re-adjust their speed accordingly. However, they cannot simply increase their speed to the maximum allowable values since they are concerned with safety, separation distance, effort, possibility of being vectored-off from landing and possibility of violating speed constraints. We present how to model these concerns and the rest of the system using semi network-from game framework. Using this framework, based on certain assumptions on pilot utility functions and on system configuration, we provide estimates of pilot behavior and overall system evolution in time. We also discuss the possible employment of this modeling tool for airspace design optimization. To support this discussion, we provide a case where we investigate the effect of increasing the merging point speed limit on the commanded speed distribution and on the percentage of vectored aircraft.

Yildiz, Yildiray; Lee, Ritchie; Brat, Guillaume

2012-01-01

307

Kretzoiarctos gen. nov., the Oldest Member of the Giant Panda Clade  

PubMed Central

The phylogenetic position of the giant panda, Ailuropoda melanoleuca (Carnivora: Ursidae: Ailuropodinae), has been one of the most hotly debated topics by mammalian biologists and paleontologists during the last century. Based on molecular data, it is currently recognized as a true ursid, sister-taxon of the remaining extant bears, from which it would have diverged by the Early Miocene. However, from a paleobiogeographic and chronological perspective, the origin of the giant panda lineage has remained elusive due to the scarcity of the available Miocene fossil record. Until recently, the genus Ailurarctos from the Late Miocene of China (ca. 8–7 mya) was recognized as the oldest undoubted member of the Ailuropodinae, suggesting that the panda lineage might have originated from an Ursavus ancestor. The role of the purported ailuropodine Agriarctos, from the Miocene of Europe, in the origins of this clade has been generally dismissed due to the paucity of the available material. Here, we describe a new ailuropodine genus, Kretzoiarctos gen. nov., based on remains from two Middle Miocene (ca. 12–11 Ma) Spanish localities. A cladistic analysis of fossil and extant members of the Ursoidea confirms the inclusion of the new genus into the Ailuropodinae. Moreover, Kretzoiarctos precedes in time the previously-known, Late Miocene members of the giant panda clade from Eurasia (Agriarctos and Ailurarctos). The former can be therefore considered the oldest recorded member of the giant panda lineage, which has significant implications for understanding the origins of this clade from a paleobiogeographic viewpoint. PMID:23155439

Abella, Juan; Alba, David M.; Robles, Josep M.; Valenciano, Alberto; Rotgers, Cheyenn; Carmona, Raül; Montoya, Plinio; Morales, Jorge

2012-01-01

308

Canibacter oris gen. nov., sp. nov., isolated from an infected human wound.  

PubMed

A facultatively anaerobic, Gram-reaction-positive, catalase- and oxidase-negative, rod-shaped bacterium isolated from an infected human wound caused by a dog bite was characterized by phenotypic and molecular genetic methods. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain IMMIB Q2029717T was a member of the order Micrococcales of the class Actinobacteria, displaying 91.6% to 96% sequence similarity with members of the family Microbacteriaceae. Phylogentic trees generated by different algorithms indicated that the strain forms an independent phylogenetic line of descent that consistently clustered proximal to the base of the genus Leucobacter. Chemical studies revealed the presence of a cell-wall murein based on L-lysine (type B1?), major menaquinone (MK-10) and a DNA G+C content of 56.9 mol%. The distinct phylogenetic position, ribotyping and matrix-assisted laser desorption/ionization time-of-flight MS profiles and the significant phenotypic differences clearly separate strain IMMIB Q2029717T from its nearest phylogenetic neighbour and support its classification as a representative of a novel genus and species, with the suggested name Canibacter oris gen. nov., sp. nov. The type strain is IMMIB Q2029717T (=DSM 27064T=CCUG 64069T). PMID:24510975

Aravena-Román, M; Inglis, T J J; Siering, C; Schumann, P; Yassin, A F

2014-05-01

309

Pelagitalea pacifica gen. nov., sp. nov., a New Marine Bacterium Isolated from Seawater.  

PubMed

A strictly aerobic, Gram-negative, beige-pigmented, short-rod-shaped, non-motile and chemoheterotrophic bacteria, designated K2-48(T) was isolated from seawater collected in the Western North Pacific Ocean near Japan. Preliminary analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Oceanospirillaceae within the class Gammaproteobacteria and that it showed the highest sequence similarity (93.7 %) to Neptunomonas qingdaonensis P10-2-4(T). The strain could be differentiated phenotypically from recognized members of the family Oceanospirillaceae. The major fatty acids of strain K2-48(T) were identified as summed feature 3 (C16:1 ?7c and/or iso-C15:0 2-OH) and C16:0 as defined by the MIDI system. The DNA G+C content was determined to be 43.2 mol%, the major respiratory quinone was identified as ubiquinone 9 and a polar lipid profile was present consisting of phosphatidylethanolamine, a phosphatidylglycerol and an unidentified phospolipid. On the basis of polyphasic taxonomic studies, it was concluded that strain K2-48(T) represents a novel genus sp. We propose the name Pelagitalea pacifica gen. nov., sp. nov. for this strain; its type strain is K2-48(T) (=KCCM 90119(T)). PMID:25487119

Lee, Hyunsang; Yoshizawa, Susumu; Kogure, Kazuhiro; Kim, Hyun Soo; Yoon, Jaewoo

2015-04-01

310

A new tardigrade, Mutaparadoxipus duodigifinis gen. nov., sp. nov. (Heterotardigrada: Arthrotardigrada), from the Southeastern United States.  

PubMed

A new genus and species of Arthrotardigrada is described from Florida, USA based on its unique adhesive pad/claw combinations. Mutaparadoxipus duodigifinis gen. nov., sp. nov., is characterized by well-developed, ventral secondary clavae that are adjacent to the mouth, pointed lateral and caudal alae, seminal receptacles with coiled ducts opening lateral to the gonopore, and all legs with digits bearing proximal adhesive pads. Distal claws are present on digits I-III of legs I-III, but are missing from digit IV. On leg IV, distal claws are present only on digits II & III. A single accessory point is present on claws II & III only. This is the fourth species discovered to date with proximal adhesive pads, increasing support for a clade of adhesive-padded arthrotardigrades, and is likely the sister taxon of Paradoxipus orzeliscoides. The incomplete set of claws may represent an evolutionary step in a progressive loss of claws hypothesized to have occurred within the Halechiniscidae. The subfamily Orzeliscinae is amended as a result. PMID:25081448

Gross, Vladimir; Miller, William R; Hochberg, Rick

2014-01-01

311

Chryseoglobus frigidaquae gen. nov., sp. nov., a novel member of the family Microbacteriaceae.  

PubMed

A motile, rod-shaped, yellow-pigmented bacterium, designated strain CW1(T), was isolated from a water-cooling system in the Republic of Korea. Cells were Gram-stain-positive, aerobic, catalase-positive and oxidase-negative. Strain CW1(T) formed slender rods with unusual bulbous protuberances. The major fatty acids were iso-C(16 : 1) (33.7 %), anteiso-C(15 : 0) (27.2 %), iso-C(14 : 0) (13.3 %) and C(16 : 0) (10.8 %). The cell-wall peptidoglycan was of type B2beta, containing lysine as the diamino acid. The respiratory quinones were menaquinones with 12, 13 and 14 isoprene units. A phylogenetic tree based on 16S rRNA gene sequences showed that strain CW1(T) formed an evolutionary lineage within the radiation enclosing members of the family Microbacteriaceae and was related to, but distant from, members of the genera Microcella and Yonghaparkia. On the basis of the evidence presented, strain CW1(T) is considered to represent a novel species of a new genus in the family Microbacteriaceae, for which the name Chryseoglobus frigidaquae gen. nov., sp. nov. is proposed. The type strain of Chryseoglobus frigidaquae is CW1(T) (=KCTC 13142(T) =JCM 14730(T)). PMID:19667371

Baik, Keun Sik; Park, Seong Chan; Kim, Ho Jun; Lee, Kang Hyun; Seong, Chi Nam

2010-06-01

312

Eoetvoesia caeni gen. nov., sp. nov., isolated from an activated sludge system treating coke plant effluent.  

PubMed

A novel bacterium, PB3-7B(T), was isolated on phenol-supplemented inorganic growth medium from a laboratory-scale wastewater purification system that treated coke plant effluent. 16S rRNA gene sequence analysis revealed that strain PB3-7B(T) belonged to the family Alcaligenaceae and showed the highest pairwise sequence similarity to Parapusillimonas granuli Ch07(T) (97.5%), Candidimonas bauzanensis BZ59(T) (97.3%) and Pusillimonas noertemannii BN9(T) (97.2%). Strain PB3-7B(T) was rod-shaped, motile and oxidase- and catalase-positive. The predominant fatty acids were C(16?:?0), C(17?:?0) cyclo, C(19?:?0) cyclo ?8c and C(14?:?0) 3-OH, and the major respiratory quinone was Q-8. The G+C content of the genomic DNA of strain PB3-7B(T) was 59.7 mol%. The novel bacterium can be distinguished from closely related type strains based on its urease activity and the capacity for assimilation of glycerol and amygdalin. On the basis of the phenotypic, chemotaxonomic and molecular data, strain PB3-7B(T) is considered to represent a new genus and species, for which the name Eoetvoesia caeni gen. nov., sp. nov. is proposed. The type strain of Eoetvoesia caeni is PB3-7B(T) (?=?DSM 25520(T)?=?NCAIM B 02512(T)). PMID:24585374

Felföldi, Tamás; Vengring, Anita; Kéki, Zsuzsa; Márialigeti, Károly; Schumann, Peter; Tóth, Erika M

2014-06-01

313

Hapsidoxylon terpsichorum gen. et sp. nov., a stem with unusual anatomy from the Triassic of Antarctica.  

PubMed

The Middle Triassic flora of the Fremouw Formation in the central Transantarctic Mountains consists of conifers, cycads, ferns, pteridosperms, and sphenophytes. Stems with an unusual anatomy have been discovered within silicified peat from the same locality. The diameters of the stems range from 1.4 to 1.7 cm; the longest specimen is approximately 12 cm. In transverse section the vascular system consists of segments that occur as single traces or are connected in the center and anastomose at varying levels within the stem. Each segment contains a bifacial vascular cambium. Secondary tissues of each segment surround a central area of parenchyma and small tracheids presumed to represent primary xylem. Surrounding the stem is a periderm. Traces are produced near the periphery of the axis and consist of radially arranged secondary xylem and a thick periderm. The absence of leaves and reproductive organs leads to uncertain phylogenetic relationships. We are unaware of any Triassic plants with this type of vascular tissue organization, and those plants with a similar type of arrangement occur only in the Devonian and Carboniferous. Possible phylogenetic affinities with the Cladoxylales and Lycophyta are examined, but the anatomical differences, along with stratigraphic age, preclude formal assignment to any known taxon at this time. Therefore, we have assigned it to a new taxon: Hapsidoxylon terpsichorum gen. et sp. nov. PMID:21665624

McManus, Hilary A; Boucher, Lisa; Taylor, Edith L; Taylor, Thomas N

2002-12-01

314

Investigating contact toxicity of Geranium and Artemisia essential oils on Bemisia tabaci Gen.  

PubMed Central

Objective: Sweet potato whitefly, Bemisia tabaci Gen. (B. tabaci), is one of the most important pests of various greenhouse crops in Iran. Nowadays, chemical insecticides are broadly used for control of the pests that causes risk to consumer's health. For the first time, contact toxicity of Pelargonium roseum Andrews and Artemisia sieberi Besser essential oils on B. tabaci and its possible application against the whitefly was evaluated in 2012. Materials and Methods: Essential oil with concentrations of 2500, 1250, 125, and 12 ppm were used. Infested leaves of greenhouse cucumber were treated by mentioned concentrations. After 24 hours, mortality of B. tabaci was recorded and compared after correcting by Abbot's formula. Results: Results showed that all concentrations of the essential oil could significantly reduce population of B. tabaci compared with the control treatment. Phytotoxicity of the treated leaves were recorded after 24, 48, and 72 hours and compared with the control. Concentrations of 2500, 1250, and 125 ppm caused severe phytotoxicity on greenhouse cucumber leaves and therefore are not suitable for greenhouse application. Phytotoxicity of 12 ppm was relatively low. Conclusions: This data implicated suitable protective effects of the essential oils to the pest infestation. Therefore, essential oils distillated from Geranium and Artemisia could be applied to control B. tabaci in greenhouse cucumber at V/V 12 ppm. PMID:25050264

Yarahmadi, Fatemeh; Rajabpour, Ali; Zandi Sohani, Nooshin; Ramezani, Leila

2013-01-01

315

Mizugakiibacter sediminis gen. nov., sp. nov., isolated from a freshwater lake.  

PubMed

A novel, moderately thermophilic, bacterial strain (skMP5(T)) was isolated from sediment of a freshwater lake in Japan. The cells were rod-shaped, motile and Gram-stain-negative. Growth was observed at temperatures ranging from 25 to 52 °C, with optimum growth observed at 48-50 °C. The pH range for growth was pH 5.0-8.2, with optimum growth at pH 6.0-7.0. The G+C content of genomic DNA was 72 mol%. The major components in the fatty acid profile were iso-C17?:?0 and iso-C17?:?1?9c. The predominant isoprenoid quinone of the strain was ubiquinone Q-8. The strain was facultatively anaerobic, and reduced nitrate to nitrite under anoxic conditions. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate was a member of the family Xanthomonadaceae within the class Gammaproteobacteria, showing highest sequence similarity with Tahibacter aquaticus RaM5-2 (93.6?%) and Metallibacterium scheffleri DKE6(T) (93.3?%). On the basis of phylogenetic and phenotypic properties, strain skMP5(T) represents a novel species of a new genus, Mizugakiibacter sediminis gen. nov., sp. nov. The type strain of the type species is skMP5(T) (?=?DSM 27098(T)?=?NBRC 109608(T)). PMID:25212223

Kojima, Hisaya; Tokizawa, Riho; Fukui, Manabu

2014-12-01

316

A new captorhinid reptile, Gansurhinus qingtoushanensis, gen. et sp. nov., from the Permian of China  

NASA Astrophysics Data System (ADS)

Captorhinids, a clade of Paleozoic reptiles, are represented by a rich fossil record that extends from the Late Carboniferous into the Late Permian. Representatives of this clade dispersed from the equatorial regions of Laurasia into the temperate regions of Pangea during the Middle and Late Permian. This rich fossil record shows that there was an evolutionary trend from faunivorous to omnivorous and herbivorous feeding habits within this clade. The discovery of well-preserved captorhinid materials in the Middle Permian of China allows us to determine that the new taxon, Gansurhinus qingtoushanensis, gen. et sp. nov, is a member of Moradisaurinae, a clade of captorhinids with multiple tooth rows arranged in parallel. The presence of this moradisaurine in the Middle Permian of south central Asia leads us to suggest that paleogeographic changes during the Permian, with part of what is today China becoming a large peninsula of Pangea, allowed these early reptiles as well as other terrestrial vertebrates to extend their geographic ranges to this region of the Late Paleozoic supercontinent.

Reisz, Robert R.; Liu, Jun; Li, Jin-Ling; Müller, Johannes

2011-05-01

317

The experimenter's museum: GenBank, natural history, and the moral economies of biomedicine.  

PubMed

Today, the production of knowledge in the experimental life sciences relies crucially on the use of biological data collections, such as DNA sequence databases. These collections, in both their creation and their current use, are embedded in the experimentalist tradition. At the same time, however, they exemplify the natural historical tradition, based on collecting and comparing natural facts. This essay focuses on the issues attending the establishment in 1982 of GenBank, the largest and most frequently accessed collection of experimental knowledge in the world. The debates leading to its creation-about the collection and distribution of data, the attribution of credit and authorship, and the proprietary nature of knowledge-illuminate the different moral economies at work in the life sciences in the late twentieth century. They offer perspective on the recent rise of public access publishing and data sharing in science. More broadly, this essay challenges the big picture according to which the rise of experimentalism led to the decline of natural history in the twentieth century. It argues that both traditions have been articulated into a new way of producing knowledge that has become a key practice in science at the beginning of the twenty-first century. PMID:21667776

Strasser, Bruno J

2011-03-01

318

Methods for Estimating Environmental Effects and Constraints on NexGen: High Density Case Study  

NASA Technical Reports Server (NTRS)

This document provides a summary of the current methods developed by Metron Aviation for the estimate of environmental effects and constraints on the Next Generation Air Transportation System (NextGen). This body of work incorporates many of the key elements necessary to achieve such an estimate. Each section contains the background and motivation for the technical elements of the work, a description of the methods used, and possible next steps. The current methods described in this document were selected in an attempt to provide a good balance between accuracy and fairly rapid turn around times to best advance Joint Planning and Development Office (JPDO) System Modeling and Analysis Division (SMAD) objectives while also supporting the needs of the JPDO Environmental Working Group (EWG). In particular this document describes methods applied to support the High Density (HD) Case Study performed during the spring of 2008. A reference day (in 2006) is modeled to describe current system capabilities while the future demand is applied to multiple alternatives to analyze system performance. The major variables in the alternatives are operational/procedural capabilities for airport, terminal, and en route airspace along with projected improvements to airframe, engine and navigational equipment.

Augustine, S.; Ermatinger, C.; Graham, M.; Thompson, T.

2010-01-01

319

Pararotylenchus n. gen. (Pararotylenchinae n. subfam., Hoplolaimidae) with Six New Species and Two New Combinations  

PubMed Central

A new subfamily, Pararotylenchinae, (Hoplolaimidae) is described. It includes a single genus, Pararotylenchus n. gen., six new species, attd two new combinations, Pararotylenchus (syn. Tylenchorhynchus) brevicaudatus (Hopper, 1959) n. comb. and Pararotylenchus (syn. Rotylenchus) pint (Mamiya, 1968) n. comb. Pararotylenchinae is similar to certain other Hoplolaimidae, such as Rotylenchinae, with respect to most characters including the short tail, the position of the phasmids near the anus, and the relatively great distance of the dorsal gland orifice from the base of the stylet knobs. The lip region, as observed with the scanning electron microscope, conforms to the basic pattern for Hoplolaimidae. The labial disc is round with slit-like amphidial openings at the lateral sides of the periphery of the disc. The distinctive anterior-most lip annule is segmented into six sectors. Unlike other Hoplolaimidae, however, the esophageal glands of Pararotylenchinae form a basal bulb similar to that of Tylenchorhynchidae; other characters do not resemble Tylencborhynchidae. Specimens of Pararotylenchus have been recovered only from cool regions at high elevations and Pacific coastal areas in the Western United States, Japan, and Korea. PMID:19300732

Baldwin, J. G.; Bell, A. H.

1981-01-01

320

Chitiniphilus shinanonensis gen. nov., sp. nov., a novel chitin-degrading bacterium belonging to Betaproteobacteria.  

PubMed

A bacterial strain capable of degrading chitin, strain SAY3T, was isolated from moat water of Ueda Castle in Nagano Prefecture, Japan. The strain was gram-negative, curved rod-shaped, facultatively anaerobic, and motile with a single polar flagellum. It grew well with chitin as a sole carbon source. The cellular fatty acids profiles showed the presence of C16:1 omega7c and C16:0 as the major components. The G+C content of DNA was 67.6 mol% and Q-8 was the major respiratory quinone. A 16S rRNA gene sequence-based phylogenetic analysis showed the strain belonged to the family Neisseriaceae but was distantly related (94% identity) to any previously known species. Since the strain was clearly distinct from closely related genera in phenotypic and chemotaxonomic characteristics, it should be classified under a new genus and a new species. We propose the name Chitiniphilus shinanonensis gen. nov., sp. nov. The type strain is SAY3T (=NBRC 104970T=NICMB 14509T). PMID:19436131

Sato, Kazuaki; Kato, Yuichi; Taguchi, Goro; Nogawa, Masahiro; Yokota, Akira; Shimosaka, Makoto

2009-04-01

321

Mobilitalea sibirica gen. nov., sp. nov., a halotolerant polysaccharide-degrading bacterium.  

PubMed

A novel strictly anaerobic, halotolerant, organotrophic bacterium, strain P3M-3(T), was isolated from a microbial mat formed under the flow of hot water emerging from a 2775 m-deep well in Tomsk region (western Siberia, Russia). Cells of strain P3M-3(T) were straight and curved rods, 0.2-0.4 µm in width and 1.5-20 µm in length. Strain P3M-3(T) grew optimally at 37 °C, pH 7.0-7.5 and in a NaCl concentration of 15 g l(-1). Under optimum growth conditions, the doubling time was 1 h. The isolate was able to ferment a variety of mono-, di- and polysaccharides, including microcrystalline cellulose. Acetate, ethanol, H2 and CO2 were the main products of glucose fermentation. The DNA G+C content was 33.4 mol%. 16S rRNA gene-based phylogenetic analysis showed that strain P3M-3(T) was a member of family Lachnospiraceae, whose representatives are also found in Clostridium cluster XIVa. 16S rRNA gene sequence similarity with Clostridium jejuense HY-35-12(T), the closest relative, was 93.9%. A novel genus and species, Mobilitalea sibirica gen. nov., sp. nov., are proposed based on phylogenetic analysis and physiological properties of the novel isolate. The type strain of the type species is P3M-3(T) (?=?DSM 26468(T)?=?VKM B-2804(T)). PMID:24827706

Podosokorskaya, O A; Bonch-Osmolovskaya, E A; Beskorovaynyy, A V; Toshchakov, S V; Kolganova, T V; Kublanov, I V

2014-08-01

322

Phytohabitans suffuscus gen. nov., sp. nov., an actinomycete of the family Micromonosporaceae isolated from plant roots.  

PubMed

An actinomycete strain, K07-0523(T), was isolated from the roots of an orchid collected in Okinawa prefecture, Japan. 16S rRNA gene sequence analysis indicated that the new strain belonged to the family Micromonosporaceae and the similarity values between strain K07-0523(T) and the type species of 24 genera in the family Micromonosporaceae were 93.3-97.7?%. Strain K07-0523(T) contained D-glutamic acid, glycine, D-alanine, meso-diaminopimelic acid, hydroxydiaminopimelic acid and L-lysine in the cell wall. The major menaquinones were MK-9(H(6)), MK-10(H(4)) and MK-10(H(6)). Galactose, glucose, mannose, ribose and xylose were present in the whole-cell sugars. The acyl type of the peptidoglycan was glycolyl. Major fatty acids were anteiso-C(17?:?0), iso-C(17?:?0), iso-C(16?:?0) and iso-C(15?:?0). Phosphatidylethanolamine was detected as the major phospholipid and corresponded to phospholipid type II. The G+C content of the genomic DNA was 73 mol%. On the basis of phylogenetic and chemotaxonomic data, the new strain represents a member of a new genus and novel species, namely Phytohabitans suffuscus gen. nov., sp. nov., in the family Micromonosporaceae. The type strain of the type species is K07-0523(T) (=DSM 45306(T)=NBRC 105367(T)). PMID:20042750

Inahashi, Yuki; Matsumoto, Atsuko; Danbara, Hirofumi; Omura, Satoshi; Takahashi, Y?ko

2010-11-01

323

Bandoniozyma gen. nov., a Genus of Fermentative and Non-Fermentative Tremellaceous Yeast Species  

PubMed Central

Background Independent surveys across the globe led to the proposal of a new basidiomycetous yeast genus within the Bulleromyces clade of the Tremellales, Bandoniozyma gen. nov., with seven new species. Methodology/Principal Findings The species were characterized by multiple methods, including the analysis of D1/D2 and ITS nucleotide sequences, and morphological and physiological/biochemical traits. Most species can ferment glucose, which is an unusual trait among basidiomycetous yeasts. Conclusions/Significance In this study we propose the new yeast genus Bandoniozyma, with seven species Bandoniozyma noutii sp. nov. (type species of genus; CBS 8364T ?=? DBVPG 4489T), Bandoniozyma aquatica sp. nov. (UFMG-DH4.20T ?=? CBS 12527T ?=? ATCC MYA-4876T), Bandoniozyma complexa sp. nov. (CBS 11570T ?=? ATCC MYA-4603T ?=? MA28aT), Bandoniozyma fermentans sp. nov. (CBS 12399T ?=? NU7M71T ?=? BCRC 23267T), Bandoniozyma glucofermentans sp. nov. (CBS 10381T ?=? NRRL Y-48076T ?=? ATCC MYA-4760T ?=? BG 02-7-15-015A-1-1T), Bandoniozyma tunnelae sp. nov. (CBS 8024T ?=? DBVPG 7000T), and Bandoniozyma visegradensis sp. nov. (CBS 12505T ?=? NRRL Y-48783T ?=? NCAIM Y.01952T). PMID:23056233

Landell, Melissa Fontes; Crestani, Juliana; Pagnocca, Fernando Carlos; Sette, Lara Durães; Passarini, Michel Rodrigo Zambrano; Rosa, Carlos Augusto; Brandão, Luciana R.; Pimenta, Raphael S.; Ribeiro, José Roberto; Garcia, Karina Marques; Lee, Ching-Fu; Suh, Sung-Oui; Péter, Gábor; Dlauchy, Dénes; Fell, Jack W.; Scorzetti, Gloria; Theelen, Bart; Vainstein, Marilene H.

2012-01-01

324

Halocafeteria seosinensis gen. et sp. nov. (Bicosoecida), a halophilic bacterivorous nanoflagellate isolated from a solar saltern.  

PubMed

Recently, heterotrophic nanoflagellates (HNF) have been reported to actively ingest prokaryotes in high salinity waters. We report the isolation and culture of an HNF from a Korean saltern pond of 300 per thousand salinity. The organism is biflagellated with an acronematic anterior flagellum and never glides on surfaces. The mitochondria have tubular cristae. Neither transitional helix nor spiral fiber were observed in the transition zones of the flagella. The cell has a cytostome supported by an arc of eight microtubules, suggesting that our isolate is a bicosoecid. Our isolate had neither mastigonemes, lorica, body scales, nor cytopharynx and thus could not be placed in any of the presently described bicosoecid genera. Phylogenetic analysis of 18S rRNA gene sequences from stramenopiles confirmed the bicosoecid affinities of our isolate, but did not place it within any established genus or family. Its closest relatives include Caecitellus and Cafeteria. The optimal range of growth temperature was 30-35 degrees C. The isolated HNF grew optimally at 150 per thousand salinity and tolerated up to 363 per thousand salinity, but it failed to grow below 75 per thousand salinity, indicating that it could be a borderline extreme halophile. On the basis of its morphological features and position in 18S rRNA trees we propose a novel genus for our isolate; Halocafeteria, n. gen. The species name Halocafeteria seosinensis sp. nov. is proposed. PMID:16874468

Park, Jong S; Cho, Byung C; Simpson, Alastair G B

2006-12-01

325

Sinibacillus soli gen. nov., sp. nov., a moderately thermotolerant member of the family Bacillaceae.  

PubMed

Two Gram-staining-positive, rod-shaped and endospore-forming bacteria that represent a single species, designated strains GD05T and GD051, were isolated from a tropical forest soil and a hot spring sediment, respectively. Cells of both strains were facultatively anaerobic, catalase- and oxidase-positive, and could grow optimally at 50 °C, pH 8.0 and with 1?% (w/v) NaCl. Analysis of the 16S rRNA gene sequence revealed that these two isolates belonged to the family Bacillaceae, but did not show sequence similarities of more than 95% to members of other related genera. The G+C content of the genomic DNA was 43.7-44.1 mol%. The major cellular fatty acids were anteiso-C15:0, iso-C15:0, iso-C16:0 and anteiso-C17:0. The main polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major menaquinone was MK-7. The peptidoglycan type was A1? (meso-diaminopimelic acid direct). On the basis of this polyphasic taxonomic analysis, the novel strains represent a novel species of a new genus in the family Bacillaceae, order Bacillales, for which the name Sinibacillus soli gen. nov., sp. nov. is proposed. The type strain is GD05T (=CCTCC AB 2013105T=KCTC 33117T). PMID:24510979

Yang, Guiqin; Zhou, Shungui

2014-05-01

326

Variibacter gotjawalensis gen. nov., sp. nov., isolated from soil of a lava forest.  

PubMed

A novel bacterial strain designated GJW-30(T) was isolated from soil of the lava forest, Gotjawal, located in Aewol, Jeju, Korea. Strain GJW-30(T) was found to be strictly aerobic, Gram-negative and to form pleomorphic, non-motile rods and white colonies on R2A agar. The major fatty acids were identified as C18:1?7c, C16:0 and C17:0, the predominant isoprenoid quinone as Q-10, the polar lipids as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid and an unidentified lipid. The cell-wall sugar pattern of strain GJW-30(T) was found to be composed of glucose, ribose and rhamnose and meso-DAP as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content of strain GJW-30(T) is 62.2 mol%. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, showed that strain GJW-30(T) forms a deep branch within the order Rhizobiales, sharing the highest level of sequence homology with Bradyrhizobium oligotrophicum LMG 10732(T) (93.6 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain GJW-30(T) is considered to represent a novel genus and species, for which the name Variibacter gotjawalensis gen. nov., sp. nov. (the type strain is GJW-30(T) = KCTC 32391(T) = CECT 8514(T) = LMG 28093(T)) is proposed. PMID:24599521

Kim, Kwang Kyu; Lee, Keun Chul; Eom, Mi Kyung; Kim, Jong-Shik; Kim, Dae-Shin; Ko, Suk-Hyung; Kim, Byung-Hyuk; Lee, Jung-Sook

2014-05-01

327

Control Control Control: A Reassessment and Comparison of GenBank and Chromatogram mtDNA Sequence Variation in Baltic Grey Seals (Halichoerus grypus)  

PubMed Central

Genetic data can provide a powerful tool for those interested in the biology, management and conservation of wildlife, but also lead to erroneous conclusions if appropriate controls are not taken at all steps of the analytical process. This particularly applies to data deposited in public repositories such as GenBank, whose utility relies heavily on the assumption of high data quality. Here we report on an in-depth reassessment and comparison of GenBank and chromatogram mtDNA sequence data generated in a previous study of Baltic grey seals. By re-editing the original chromatogram data we found that approximately 40% of the grey seal mtDNA haplotype sequences posted in GenBank contained errors. The re-analysis of the edited chromatogram data yielded overall similar results and conclusions as the original study. However, a significantly different outcome was observed when using the uncorrected dataset based on the GenBank haplotypes. We therefore suggest disregarding the existing GenBank data and instead using the correct haplotypes reported here. Our study serves as an illustrative example reiterating the importance of quality control through every step of a research project, from data generation to interpretation and submission to an online repository. Errors conducted in any step may lead to biased results and conclusions, and could impact management decisions. PMID:23977362

Fietz, Katharina; Graves, Jeff A.; Olsen, Morten Tange

2013-01-01

328

GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update  

PubMed Central

Summary: GenAlEx: Genetic Analysis in Excel is a cross-platform package for population genetic analyses that runs within Microsoft Excel. GenAlEx offers analysis of diploid codominant, haploid and binary genetic loci and DNA sequences. Both frequency-based (F-statistics, heterozygosity, HWE, population assignment, relatedness) and distance-based (AMOVA, PCoA, Mantel tests, multivariate spatial autocorrelation) analyses are provided. New features include calculation of new estimators of population structure: G?ST, G??ST, Jost’s Dest and F?ST through AMOVA, Shannon Information analysis, linkage disequilibrium analysis for biallelic data and novel heterogeneity tests for spatial autocorrelation analysis. Export to more than 30 other data formats is provided. Teaching tutorials and expanded step-by-step output options are included. The comprehensive guide has been fully revised. Availability and implementation: GenAlEx is written in VBA and provided as a Microsoft Excel Add-in (compatible with Excel 2003, 2007, 2010 on PC; Excel 2004, 2011 on Macintosh). GenAlEx, and supporting documentation and tutorials are freely available at: http://biology.anu.edu.au/GenAlEx. Contact: rod.peakall@anu.edu.au PMID:22820204

Peakall, Rod; Smouse, Peter E.

2012-01-01

329

Description of Alloprevotella rava gen. nov., sp. nov., isolated from the human oral cavity, and reclassification of Prevotella tannerae Moore et al. 1994 as Alloprevotella tannerae gen. nov., comb. nov.  

PubMed Central

Five strains of anaerobic, Gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group. Phylogenetic analysis of full-length 16S rRNA gene sequences showed that these strains represented a novel group within the family Prevotellaceae, and the most closely related species was Prevotella tannerae. P. tannerae and the novel taxon are deeply branched from the genus Prevotella, with sequence identities to the type strain of the type species of Prevotella, Prevotella melaninogenica, of 82.2 and 85.6?%, respectively. The novel genus Alloprevotella gen. nov. is proposed to accommodate the novel species Alloprevotella rava gen. nov., sp. nov. and the previously named Prevotella tannerae Moore et al. 1994 as Alloprevotella tannerae gen. nov., comb. nov. The type species is Alloprevotella tannerae. The type strain of Alloprevotella rava is 81/4-12T (?=?DSM 22548T ?=?CCUG 58091T) and the type strain of Alloprevotella tannerae is ATCC 51259T ?=?CCUG 34292T ?=?CIP 104476T ?=?NCTC 13073T. Alloprevotella rava is weakly to moderately saccharolytic and produces moderate amounts of acetic acid and major amounts of succinic acid as end products of fermentation. Strains are sensitive to 20?% bile and hydrolyse gelatin. The principal cellular long-chain fatty acids are anteiso-C15?:?0, iso-C15?:?0, C16?:?0, iso-C17?:?0 and iso-C17?:?0 3-OH. The G+C content of the DNA of the type strain is 47 mol%. PMID:22753527

Downes, Julia; Dewhirst, Floyd E.; Tanner, Anne C. R.

2013-01-01

330

Crenobacter luteus gen. nov., sp. nov., isolated from a hot spring.  

PubMed

A slightly thermophilic, Gram-staining-negative and strictly aerobic bacteria, designated strain YIM 78141(T), was isolated from a sediment sample collected at Hehua hot spring, Tengchong, Yunnan province, south-west China. Cells of the strain were short-rod-shaped and colonies were yellowish and circular. The strain grew at pH 6.0-10.0 (optimum, pH 8.0-9.0) and 10-55 °C (optimum, 40-50 °C). Phylogenetic analyses based on 16S rRNA gene sequence comparison demonstrated that strain YIM 78141(T) belongs to the family Neisseriaceae, and strain YIM 78141(T) also showed low levels of 16S rRNA gene sequence similarity (below 93.4%) with all other genera in this family. The only quinone was ubiquinone 8 and the genomic DNA G+C content was 67.3 mol%. Major fatty acids (>5%) were C12:0, C16:0, C18:1?7c and summed feature 3. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phospholipids of unknown structure containing aminoglycophospholipid and three unidentified polar lipids. On the basis of the morphological, physiological and biochemical characteristics as well as genotypic data, this strain should be classified as a representative of a novel genus and species of the family Neisseriaceae, for which the name Crenobacter luteus gen. nov., sp. nov. is proposed. The type strain is YIM 78141(T) (?=BCRC 80650(T)?=KCTC 32558(T)?=DSM 27258(T)). PMID:25332210

Dong, Lei; Ming, Hong; Zhou, En-Min; Yin, Yi-Rui; Liu, Lan; Feng, Hui-Geng; Xian, Wen-Dong; Nie, Guo-Xing; Li, Wen-Jun

2015-01-01

331

Visual Advantage of Enhanced Flight Vision System During NextGen Flight Test Evaluation  

NASA Technical Reports Server (NTRS)

Synthetic Vision Systems and Enhanced Flight Vision System (SVS/EFVS) technologies have the potential to provide additional margins of safety for aircrew performance and enable operational improvements for low visibility operations in the terminal area environment. Simulation and flight tests were jointly sponsored by NASA's Aviation Safety Program, Vehicle Systems Safety Technology project and the Federal Aviation Administration (FAA) to evaluate potential safety and operational benefits of SVS/EFVS technologies in low visibility Next Generation Air Transportation System (NextGen) operations. The flight tests were conducted by a team of Honeywell, Gulfstream Aerospace Corporation and NASA personnel with the goal of obtaining pilot-in-the-loop test data for flight validation, verification, and demonstration of selected SVS/EFVS operational and system-level performance capabilities. Nine test flights were flown in Gulfstream's G450 flight test aircraft outfitted with the SVS/EFVS technologies under low visibility instrument meteorological conditions. Evaluation pilots flew 108 approaches in low visibility weather conditions (600 feet to 3600 feet reported visibility) under different obscurants (mist, fog, drizzle fog, frozen fog) and sky cover (broken, overcast). Flight test videos were evaluated at three different altitudes (decision altitude, 100 feet radar altitude, and touchdown) to determine the visual advantage afforded to the pilot using the EFVS/Forward-Looking InfraRed (FLIR) imagery compared to natural vision. Results indicate the EFVS provided a visual advantage of two to three times over that of the out-the-window (OTW) view. The EFVS allowed pilots to view the runway environment, specifically runway lights, before they would be able to OTW with natural vision.

Kramer, Lynda J.; Harrison, Stephanie J.; Bailey, Randall E.; Shelton, Kevin J.; Ellis, Kyle K.

2014-01-01

332

Feasibility of a Networked Air Traffic Infrastructure Validation Environment for Advanced NextGen Concepts  

NASA Technical Reports Server (NTRS)

Abstract-Next Generation Air Transportation System (NextGen) applications reliant upon aircraft data links such as Automatic Dependent Surveillance-Broadcast (ADS-B) offer a sweeping modernization of the National Airspace System (NAS), but the aviation stakeholder community has not yet established a positive business case for equipage and message content standards remain in flux. It is necessary to transition promising Air Traffic Management (ATM) Concepts of Operations (ConOps) from simulation environments to full-scale flight tests in order to validate user benefits and solidify message standards. However, flight tests are prohibitively expensive and message standards for Commercial-off-the-Shelf (COTS) systems cannot support many advanced ConOps. It is therefore proposed to simulate future aircraft surveillance and communications equipage and employ an existing commercial data link to exchange data during dedicated flight tests. This capability, referred to as the Networked Air Traffic Infrastructure Validation Environment (NATIVE), would emulate aircraft data links such as ADS-B using in-flight Internet and easily-installed test equipment. By utilizing low-cost equipment that is easy to install and certify for testing, advanced ATM ConOps can be validated, message content standards can be solidified, and new standards can be established through full-scale flight trials without necessary or expensive equipage or extensive flight test preparation. This paper presents results of a feasibility study of the NATIVE concept. To determine requirements, six NATIVE design configurations were developed for two NASA ConOps that rely on ADS-B. The performance characteristics of three existing in-flight Internet services were investigated to determine whether performance is adequate to support the concept. Next, a study of requisite hardware and software was conducted to examine whether and how the NATIVE concept might be realized. Finally, to determine a business case, economic factors were evaluated and a preliminary cost-benefit analysis was performed.

McCormack, Michael J.; Gibson, Alec K.; Dennis, Noah E.; Underwood, Matthew C.; Miller,Lana B.; Ballin, Mark G.

2013-01-01

333

Natranaerobaculum magadiense gen. nov., sp. nov., an anaerobic, alkalithermophilic bacterium from soda lake sediment.  

PubMed

An obligately alkaliphilic, anaerobic, thermo- and halotolerant, spore-forming bacterium was isolated from sediments of soda lake Magadi (Kenya) and designated strain Z-1001(T). Cells of strain Z-1001(T) were straight, Gram-positive rods, slowly motile. Strain Z-1001(T) was found to be an obligate anaerobe. It grew within a pH range from 7.5 to 10.7 with an optimum at 9.25-9.5 (at 40 °C), a temperature range from 20 to 57 °C with an optimum at 45-50 °C, and a NaCl concentration range from 0 to 1.55 M with an optimum at 1.2-1.4 M. Peptides, such as meat and yeast extracts, peptone and tryptone, were fermented by Z-1001(T). Carbohydrates did not support growth. With yeast extract as an electron donor, strain Z-1001(T) reduced S(2)O(3)(2-), NO(-)(3), AsO(3-)(4), Fe(III) citrate and anthraquinone-2,6-disulfonate (AQDS) as electron acceptors. The isolate was able to grow oligotrophically with a very small amount of yeast extract: 0.03 g l(-1). The main fatty acids were C16?:?0, C16?:?1?7c, C18?:?0 and C18?:?1?9. The DNA G+C content of the isolate was 35.6 mol%. 16S rRNA gene sequence analysis showed that strain Z-1001(T) is a member of family Natranaerobiaceae, clustering with the type strain of Natranaerobius thermophilus (95.8-96.0?% sequence similarity). On the basis of physiological and phylogenetic data it is proposed that strain Z-1001(T) (?=?DSM 24923(T)?=?VKM B-2666(T)) represents a novel genus and species, Natranaerobaculum magadiense gen. nov., sp. nov. PMID:23859946

Zavarzina, Daria G; Zhilina, Tatyana N; Kuznetsov, Boris B; Kolganova, Tatyana V; Osipov, Georgy A; Kotelev, Mikhail S; Zavarzin, Georgy A

2013-12-01

334

Fonticella tunisiensis gen. nov., sp. nov., isolated from a hot spring.  

PubMed

A strictly anaerobic, moderately thermophilic, halotolerant rod, designated BELH25(T), was isolated from a water sample of a Tunisian hot spring. Cells were non-motile, 2-6 µm long and 0.4-0.6 µm wide, appearing singly or in pairs. The isolate grew at 45-70 °C (optimum 55 °C), at pH 6.2-8.0 (optimum pH 7.0) and with 0-4% NaCl (optimum 0-2.0%). Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Strain BELH25(T) used cellobiose, fructose, galactose, glucose, maltose, mannose, sucrose, starch and yeast extract as electron donors. The main fermentation products from glucose metabolism were formate, acetate, ethanol and CO2. The predominant cellular fatty acids were iso-C15:0, iso-C17:0 and anteiso-C15:0. The DNA G+C content was 37.2 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain BELH25(T) was most closely related to Caloramator viterbiensis JW/MS-VS5(T) and Fervidicella metallireducens AeB(T) (92.2 and 92.1% sequence similarity, respectively), and the isolate was positioned approximately equidistantly between these genera. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, strain BELH25(T) is proposed to be a member of a novel species of a novel genus within the order Clostridiales, family Clostridiaceae, for which the name Fonticella tunisiensis gen. nov., sp. nov. is proposed. The type strain of the type species is BELH25(T) (=DSM 24455(T)=JCM 17559(T)). PMID:23024143

Fraj, Belkis; Ben Hania, Wajdi; Postec, Anne; Hamdi, Moktar; Ollivier, Bernard; Fardeau, Marie-Laure

2013-06-01

335

Anaerosalibacter bizertensis gen. nov., sp. nov., a halotolerant bacterium isolated from sludge.  

PubMed

A strictly anaerobic, halotolerant and thermotolerant strain, designated C5BEL(T), was isolated in north Tunisia from storage tanks holding waste generated by the recycling of discarded motor oils. Cells of strain C5BEL(T) were Gram-stain-positive, motile by laterally inserted flagella, straight, and spore-forming. Their two major fatty acids were iso-C(15?:?0) and iso-C(15?:?0) dimethyl acetal. Growth was observed at temperatures of 25-55 °C (optimum, 40 °C) and at pH 6-9 (optimum, pH 7.5). The salinity range for growth was 0-100 g l(-1) NaCl (optimum, 5 g l(-1)). Yeast extract was required for growth. Strain C5BEL(T) was heterotrophic, able to use glucose, pyruvate, succinate, yeast extract, bio-trypticase and peptone, but unable to grow on Casamino acids. Sulfate, thiosulfate, sulfite, elemental sulfur, fumarate, nitrate and nitrite were not reduced. The DNA G+C content of strain C5BEL(T) was 31.1 mol%. 16S rRNA gene sequence analysis indicated that strain C5BEL(T) was a member of the family Clostridiaceae, class Clostridia, phylum Firmicutes and was most closely related to Sporanaerobacter acetigenes Lup33(T) (?=?DSM 13106(T)) (92.4?% similarity). On the basis of 16S rRNA gene sequence comparisons and physiological characteristics, strain C5BEL(T) can be classified as a novel species in a new genus, for which the name Anaerosalibacter bizertensis gen. nov., sp. nov. is proposed. The type strain of the type species is C5BEL(T) (?=?DSM 23801(T)?=?JCM 17239(T)). PMID:22140152

Rezgui, Raja; Maaroufi, Abderrazak; Fardeau, Marie-Laure; Ben Ali Gam, Zouhaier; Cayol, Jean-Luc; Ben Hamed, Saïd; Labat, Marc

2012-10-01

336

Chryseomicrobium imtechense gen. nov., sp. nov., a new member of the family Planococcaceae.  

PubMed

A Gram-stain-positive, rod-shaped, yellow, non-motile, non-spore-forming, strictly aerobic bacterial strain, designated MW 10(T), was isolated from seawater of the Bay of Bengal, India, and was subjected to a polyphasic taxonomic study. Analysis of the 16S rRNA gene sequence revealed that strain MW 10(T) showed highest similarity to the type strains of Psychrobacillus psychrodurans (96.15 %) and Psychrobacillus psychrotolerans (96.01 %) and showed less than 96 % similarity to members of the genera Paenisporosarcina, Planococcus, Sporosarcina and Planomicrobium. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain MW 10(T) formed a clade separate from members of closely related genera. The morphological, physiological and chemotaxonomic characteristics of strain MW 10(T) differed from those of members of closely related genera. The major fatty acid in strain MW 10(T) was iso-C(15 : 0) and the menaquinones were MK-7 (48.4 %), MK-8 (32.3 %), MK-7(H(2)) (13.7 %) and MK-6 (5.6 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unknown phospholipid, an unknown lipid and an unknown glycolipid. The cell-wall peptidoglycan type was l-Lys-d-Asp. The genomic DNA G+C content (53.4 mol%) of strain MW 10(T) was significantly different from those of members of closely related genera. On the basis of its morphological, physiological and chemotaxonomic characteristics as well as our phylogenetic analysis, we conclude that strain MW 10(T) is a member of a novel genus and species, for which the name Chryseomicrobium imtechense gen. nov., sp. nov., is proposed. The type strain of Chryseomicrobium imtechense is MW 10(T) (?= MTCC 10098(T) ?= JCM 16573(T)). PMID:20833890

Arora, Pankaj Kumar; Chauhan, Archana; Pant, Bhawana; Korpole, Suresh; Mayilraj, Shanmugam; Jain, Rakesh Kumar

2011-08-01

337

Halovenus aranensis gen. nov., sp. nov., an extremely halophilic archaeon from Aran-Bidgol salt lake.  

PubMed

A novel red-pigmented halophilic archaeon, strain EB27(T), was isolated from Aran-Bidgol salt lake, a hypersaline playa in Iran. Cells of strain EB27(T) were non-motile and pleomorphic (rods to triangular or disc-shaped). Strain EB27(T) required at least 2.5 M NaCl and 0.1 M MgCl(2) for growth. Optimal growth was achieved at 4 M NaCl and 0.5 M MgCl(2). The optimum pH and temperature for growth were pH 7.5 and 40 °C; it was able to grow at pH 6.0-8.0 and 25-50 °C. 16S rRNA gene sequence analysis showed that strain EB27(T) is a member of the family Halobacteriaceae; however, levels of 16S rRNA gene sequence similarity were as low as 90.0, 89.3 and 89.1 % to the most closely related haloarchaeal taxa, namely Halalkalicoccus tibetensis DS12(T), Halosimplex carlsbadense 2-9-1(T) and Halorhabdus utahensis AX-2(T), respectively. The DNA G+C content of strain EB27(T) was 61 mol%. Strain EB27(T) contained phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, common phospholipids found in haloarchaea, together with two minor phospholipids. The only quinone present was MK-8(II-H(2)). Physiological, biochemical and phylogenetic differences between strain EB27(T) and recognized genera of extremely halophilic archaea suggest that this strain represents a novel species in a new genus within the family Halobacteriaceae, for which the name Halovenus aranensis gen. nov., sp. nov. is proposed. The type strain of Halovenus aranensis, the type species of the new genus, is strain EB27(T) ( = IBRC-M 10015(T) = CGMCC 1.11001(T)). PMID:21828022

Makhdoumi-Kakhki, A; Amoozegar, M A; Ventosa, A

2012-06-01

338

Halopenitus persicus gen. nov., sp. nov., an archaeon from an inland salt lake.  

PubMed

A novel pale pink-pigmented halophilic archaeon, strain DC30(T), was isolated from Aran-Bidgol salt lake, a hypersaline playa in Iran. Cells of strain DC30(T) were non-motile and pleomorphic, from rods to triangular or disc-shaped. Strain DC30(T) required at least 1.7 M NaCl and 0.05 M MgCl(2) for growth (optimum, 3 M NaCl and 0.1 M MgCl(2)). The optimum pH and temperature for growth of strain DC30(T) were pH 7.5 and 40 °C, respectively, although it was capable of growth over pH and temperature ranges of 6.5-8.5 and 25-50 °C, respectively. Analysis of the 16S rRNA gene sequence showed that strain DC30(T) was a member of the family Halobacteriaceae. However, it had low 16S rRNA gene sequence similarities of 92.4%, 89.4% and 89.1% to the most closely related haloarchaeal taxa, the type species of the genera Halorubrum, Halogranum and Haloplanus, respectively. The DNA G+C content was 66.0 mol%. Phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, common phospholipids found in haloarchaea, were present. Three minor phospholipids and one unidentified glycolipid were also observed. The only quinone present was MK-8(II-H(2)). The physiological, biochemical and phylogenetic differences between strain DC30(T) and other previously described genera of extremely halophilic archaea suggest that strain DC30(T) represents a novel species in a new genus within the family Halobacteriaceae, for which the name Halopenitus persicus gen. nov., sp. nov. is proposed. The type strain of Halopenitus persicus is DC30(T) (?=?IBRC 10041(T)?=?KCTC 4046(T)). PMID:22003044

Amoozegar, Mohammad Ali; Makhdoumi-Kakhki, Ali; Shahzadeh Fazeli, Seyed Abolhassan; Azarbaijani, Reza; Ventosa, Antonio

2012-08-01

339

Namhaeicola litoreus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from seawater.  

PubMed

A Gram-staining-negative, non-flagellated, non-gliding and pleomorphic bacterial strain, designated DPG-25(T), was isolated from seawater in a seaweed farm in the South Sea in Korea and its taxonomic position was investigated by using a polyphasic approach. Strain DPG-25(T) grew optimally at 25 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. Flexirubin-type pigments were not produced. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DPG-25(T) formed a cluster with the type strains of Actibacter sediminis, Aestuariicola saemankumensis and Lutimonas vermicola. Strain DPG-25(T) exhibited 16S rRNA gene sequence similarity values of 95.3, 93.1 and 93.6?% to the type strains of Actibacter sediminis, Aestuariicola saemankumensis and L. vermicola, respectively. Strain DPG-25(T) contained MK-6 as the predominant menaquinone and iso-C(15 : 0) and iso-C(17 : 0) 3-OH as the major fatty acids. The major polar lipids detected in strain DPG-25(T) were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content was 39.9 mol%. Differential phenotypic properties and the phylogenetic distinctiveness of strain DPG-25(T) demonstrated that this strain is distinguishable from Actibacter sediminis, Aestuariicola saemankumensis and L. vermicola. On the basis of the data presented here, strain DPG-25(T) represents a novel species in a novel genus of the family Flavobacteriaceae, for which the name Namhaeicola litoreus gen. nov., sp. nov. is proposed. The type strain of Namhaeicola litoreus is DPG-25(T) (= KCTC 23702(T) = CCUG 61485(T)). PMID:22058323

Jung, Yong-Taek; Kim, Ji-Hoon; Kang, So-Jung; Oh, Tae-Kwang; Yoon, Jung-Hoon

2012-09-01

340

Boseongicola aestuarii gen. nov., sp. nov., isolated from a tidal flat sediment.  

PubMed

A Gram-stain-negative, aerobic, non-motile and pleomorphic (coccoid, ovoid or rod-shaped) bacterial strain, BS-W15(T), isolated from a tidal flat sediment at Boseong in South Korea, was characterized taxonomically. Strain BS-W15(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of approximately 2.0% (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees, based on 16S rRNA gene sequences, revealed that strain BS-W15(T) joined the cluster comprising the type strains of Profundibacterium mesophilum, Hwanghaeicola aestuarii, M. pelagius and M. salinus, showing 93.5-96.4% sequence similarities. Strain BS-W15(T) contained Q-10 as the predominant ubiquinone and C(18?:?1)?7c as the predominant fatty acid. The polar lipid profile of strain BS-W15(T) contained phosphatidylcholine and phosphatidylglycerol as major components, differentiating it from those of the type strains of P. mesophilum, H. aestuarii, M. pelagius and M. salinus. The DNA G+C content of strain BS-W15(T) was 58.7 mol%. The differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrate that strain BS-W15(T) is distinct from type strains of P. mesophilum, H. aestuarii, M. pelagius and M. salinus. On the basis of the data presented, strain BS-W15(T) is considered to represent a novel genus and species, for which the name Boseongicola aestuarii gen. nov., sp. nov. is proposed. The type strain is BS-W15(T) (?=?KCTC 32576(T)?=?CECT 8489(T)). PMID:24824636

Park, Sooyeon; Park, Ja-Min; Lee, Keun-Chul; Bae, Kyung Sook; Yoon, Jung-Hoon

2014-08-01

341

Falsirhodobacter halotolerans gen. nov., sp. nov., isolated from dry soils of a solar saltern.  

PubMed

Two bacterial strains (JA744(T) and JA745) were isolated from dry soil samples collected from solar salterns at Humma, Odisha, India. Both strains were Gram-stain-negative, catalase- and oxidase-positive, motile rods. Major fatty acids in both strains included C18:1?7c, C18:0 and C16:0, while minor amounts of C10:0 3-OH, C12:0, C12:0 3-OH, C14:0 and C16:0 were also present. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, an unidentified glycolipid, five unidentified lipids, an unidentified aminolipid and an unidentified phospholipid made up the polar lipids of both strains. Both strains had bacteriohopane derivatives (BHD1,2) and diploptene as major hopanoids. Mean genomic DNA G+C content was 75 ± 1 mol% and the two strains were closely related (mean DNA-DNA hybridization >90%). Phylogenetic analysis based on the 16S rRNA gene sequence showed that the two strains clustered with species of the genus Rhodobacter belonging to the family Rhodobacteraceae of the class Alphaproteobacteria. The highest sequence similarity was observed with Rhodobacter sphaeroides ATH2.4.1(T) (96%) and other members of the genera Rhodobacter and Pseudorhodobacter (<96%). However, the two strains were positioned distinctly outside the group formed by the other genera of the family Rhodobacteraceae. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of these isolates as representatives of a novel species in a new genus, for which the name Falsirhodobacter halotolerans gen. nov., sp. nov. is proposed. The type strain of Falsirhodobacter halotolerans is JA744(T) (=KCTC 32158(T) =NBRC 108897(T)). PMID:23104358

Subhash, Y; Tushar, L; Sasikala, Ch; Ramana, Ch V

2013-06-01

342

Imtechella halotolerans gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from estuarine water.  

PubMed

A novel Gram-negative, rod-shaped, non-motile, non-sporulating bacterium, designated strain K1(T), was isolated from an estuarine water sample collected from Kochi, Kerala, India. Colonies on marine agar were circular, 2.0-2.5 mm in diameter, shiny, yellow, translucent and convex with entire margins. Strain K1(T) was negative for ornithine decarboxylase, lysine decarboxylase, nitrate reduction and H(2)S production. The fatty acids were dominated by iso-branched components with a high abundance of iso-C(15:0), iso-C(15:1) G and iso-C(17:0) 3-OH; MK-6 (64%) and MK-7 (34%) were found as major respiratory quinones; and phosphatidylethanolamine, two unidentified aminolipids, four unidentified phospholipids and two unidentified lipids were major polar lipids. The DNA G+C content of strain K1(T) was 46.1 mol%. 16S rRNA gene sequence analysis indicated that strain K1(T) was related most closely to the type strain of Zhouia amylolytica (pairwise sequence similarity of 93.0%). Phylogenetic analysis showed that strain K1(T) formed a distinct branch within the family Flavobacteriaceae and clustered with the clade comprising species of the genera Zhouia, Coenonia and Capnocytophaga, being phylogenetically most closely related to the type strain of Zhouia amylolytica at a distance of 9.2% (90.8% similarity). Other species of the genera within the same clade were related to strain K1(T) at distances of 15.0-23.1%. Based on phenotypic and chemotaxonomic characteristics and on phylogenetic inference, strain K1(T) is considered to represent a novel species of a new genus in the family Flavobacteriaceae, for which the name Imtechella halotolerans gen. nov., sp. nov. is proposed. The type strain of Imtechella halotolerans is K1(T) (=MTCC 11055(T)=JCM 17677(T)). PMID:22199208

Surendra, Vikram; Bhawana, Pant; Suresh, Korpole; Srinivas, T N R; Kumar, Pinnaka Anil

2012-11-01

343

Spinactinospora alkalitolerans gen. nov., sp. nov., an actinomycete isolated from marine sediment.  

PubMed

A novel marine actinomycete, designated CXB654(T), was isolated from marine sediment collected at a depth of 17.5 m near the Yellow Sea Cold Water Mass, China. Optimal growth occurred at 37.0 °C, at pH 7.0-8.0 and in 3-8% (w/v) NaCl. Strain CXB654(T) formed branched substrate mycelium without fragmentation. Abundant aerial mycelium differentiated into long or short chains of spores and spores were elliptical and cylindrical with spiny surfaces. Strain CXB654(T) contained meso-diaminopimelic acid as the diagnostic diamino acid, and ribose and glucose as the major whole-cell components. Phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. MK-10(H(8)), MK-10(H(6)) and MK-9(H(8)) were the predominant menaquinones. The major fatty acids were i-C(16:0) (24.46%), ai-C(17:0) (20.66%) and C(18:0) (20.14%). The DNA G+C content was 71.1 mol%. Comparative analysis of 16S rRNA gene sequences showed that the novel strain was most closely related to genera within the family Nocardiopsaceae, but formed a separate lineage. Highest sequence similarities were to Murinocardiopsis flavida DSM 45312(T) (96.6%), Thermobifida halotolerans YIM 90462(T) (96.5%) and Marinactinospora thermotolerans DSM 45154(T) (96.1%). On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain CXB654(T) was considered to represent a novel species in a new genus in the family Nocardiopsaceae, and the name Spinactinospora alkalitolerans gen. nov., sp. nov. is proposed; the type strain is CXB654(T) (=DSM 45414(T)=LMG 25485(T)). PMID:21216917

Chang, Xianbo; Liu, Wenzheng; Zhang, Xiao-Hua

2011-12-01

344

Sinomicrobium oceani gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment.  

PubMed

A marine bacterium, designated SCSIO 03483(T), was isolated from a marine sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or marine agar 2216. Optimal growth occurred in the presence of 0-4?% (w/v) NaCl, at pH 7.0 and a temperature range of 28-37 °C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarity with Imtechella halotolerans K1(T) (92.7?%). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera Imtechella, Joostella and Zhouia. Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C15?:?0, iso-C17?:?0 3-OH and summed feature 3 (C16?:?1?6c/C16?:?1?7c). The DNA G+C content of strain SCSIO 03483(T) was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483(T) represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain of Sinobacterium oceani is SCSIO 03483(T) (?=?KCTC 23994(T)?=?CGMCC 1.12145(T)). PMID:22707529

Xu, Ying; Tian, Xin-Peng; Liu, Yu-Juan; Li, Jie; Kim, Chang-Jin; Yin, Hao; Li, Wen-Jun; Zhang, Si

2013-03-01

345

Visual advantage of enhanced flight vision system during NextGen flight test evaluation  

NASA Astrophysics Data System (ADS)

Synthetic Vision Systems and Enhanced Flight Vision System (SVS/EFVS) technologies have the potential to provide additional margins of safety for aircrew performance and enable operational improvements for low visibility operations in the terminal area environment. Simulation and flight tests were jointly sponsored by NASA's Aviation Safety Program, Vehicle Systems Safety Technology project and the Federal Aviation Administration (FAA) to evaluate potential safety and operational benefits of SVS/EFVS technologies in low visibility Next Generation Air Transportation System (NextGen) operations. The flight tests were conducted by a team of Honeywell, Gulfstream Aerospace Corporation and NASA personnel with the goal of obtaining pilot-in-the-loop test data for flight validation, verification, and demonstration of selected SVS/EFVS operational and system-level performance capabilities. Nine test flights were flown in Gulfstream's G450 flight test aircraft outfitted with the SVS/EFVS technologies under low visibility instrument meteorological conditions. Evaluation pilots flew 108 approaches in low visibility weather conditions (600 feet to 3600 feet reported visibility) under different obscurants (mist, fog, drizzle fog, frozen fog) and sky cover (broken, overcast). Flight test videos were evaluated at three different altitudes (decision altitude, 100 feet radar altitude, and touchdown) to determine the visual advantage afforded to the pilot using the EFVS/Forward-Looking InfraRed (FLIR) imagery compared to natural vision. Results indicate the EFVS provided a visual advantage of two to three times over that of the out-the-window (OTW) view. The EFVS allowed pilots to view the runway environment, specifically runway lights, before they would be able to OTW with natural vision.

Kramer, Lynda J.; Harrison, Stephanie J.; Bailey, Randall E.; Shelton, Kevin J.; Ellis, Kyle K. E.

2014-06-01

346

Jilinibacillus soli gen. nov., sp. nov., a novel member of the family Bacillaceae.  

PubMed

A Gram-positive, aerobic, rod-shaped, motile, endospore-forming bacterium, designated strain A12(T), was isolated from a saline and alkali soil samples in Baicheng City, western of Jilin Province, China. Growth occurred in 15-45 °C (optimum, 30 °C) and at pH 7.0-11.5 (optimum, pH 9.0) and in the presence of 0-10 % (w/v) NaCl [optimum, 1-3 % (w/v) NaCl]. Meso-DAP was present in the peptidoglycan. The predominant menaquinone was MK-7. The major polar lipid profile was phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidyl inositol-methyl and phosphotidylinositol dimannosid. The major fatty acid (>10 % of total fatty acids) was anteiso-C15:0. DNA G + C content was 36.2 mol %. The level of 16S rRNA gene sequence similarity between strain A12(T) and other recognized species of the family was below 95.6 %. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that the strain A12(T) fell with the family Bacillaceae and formed a distinct taxon. Based on physiological, chemotaxonomic and phylogenetic analyses, strain A12(T) was considered to represent a novel species of a new genus, for which the name Jilinibacillus soli gen. nov., sp. nov. was proposed. The type strain of Jilinibacillus soli was A12(T) (=GIMN1.014(T) = CCTCC M2011164(T) = KCTC 33417(T)). PMID:25193716

Liu, Jingying; Wang, Xiuran; Li, Meina; Du, Qian; Li, Qiyun; Ma, Pengda

2015-01-01

347

Pseudoscillatoria coralii gen. nov., sp. nov., a cyanobacterium associated with coral black band disease (BBD).  

PubMed

Black band disease (BBD) is a widespread coral disease which mainly infects massive framework-building corals. BBD is believed to be caused by a consortium of microorganisms and may not result from the actions of a primary pathogen. The BBD microbial community is dominated, in terms of biomass, by filamentous cyanobacteria. Here we describe a cyanobacterial strain, designated BgP10_4S(T), cultured from a BBD-affected Favia sp. 25 degreesoal from the northern Red Sea (Gulf of Eilat, Israel). This dark-green pigmented cyanobacterium showed optimal growth at salinities of 5.0 to 5.5% (w/v), pH of 7 to 8 and cultivation temperatures of 25 0C. Morphological examination revealed cylindrical, unbranched trichomes with tapering and blunt cells at the ends which leave a thin mucilaginous trail as they glide. No sheath was evident under these conditions. Inclusion bodies and straight thylakoids were clearly discerned by transmission electron microscopy. Pigment analysis revealed absorption spectra for phycocyanin, carotenoid and chlorophyll a. The sequence of the 16S rRNA gene in this cyanobac(t)erium isolate showed high similarity (99%) to cyanobacterial sequences retrieved from BBD-affected corals from different geographical sites (i.e. the Caribbean Sea, Palau and the Red Sea). The BgP10_4ST strain is observed to be a persisten(t) component of the BBD mat of Faviid corals and may thus be an important agent in the disease etiology. On the basis (of its morphological, physiological and phylogenetic distinctiveness, strain BgP10_4ST represents a novel genus and species of Subsection III (formerly Oscillatoriales), for which the name Pseudoscillatoria coralii gen. nov., sp. nov. is proposed. PMID:20095244

Rasoulouniriana, Diana; Siboni, Nachshon; Ben-Dov, Eitan; Kramarsky-Winter, Esti; Loya, Yossi; Kushmaro, Ariel

2009-11-16

348

Emcibacter nanhaiensis gen. nov. sp. nov., isolated from sediment of the South China Sea.  

PubMed

A new aerobic, Gram stain-negative, oxidase- and catalase- positive, ovoid or rod-shaped bacterial strain, designated HTCJW17(T), was isolated from a sediment collected from the South China Sea and subjected to a polyphasic taxonomic characterization. The isolate forms small, creamy-white, opaque and circular colonies on agar plates. Growth occurs between 15 and 45 °C, 1-7 % (w/v) NaCl and pH 6-9. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HTCJW17(T) belongs to the family Kordiimonadaceae of the order Kordiimonadales, with highest sequence similarity to Kordiimonas gwangyangensis GW14-5(T) (91.1 %). The major fatty acids were identified to be summed feature 8 (C18:1 ?7c/?6c; 56.6 %), C14:0 (8.5 %), C14:0 2-OH (8.4 %), summed feature 3 (C16:1 ?7c/C16:1 ?6c; 6.2 %), and C16:0 (5.2 %). The polar lipids were determined to be phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, six unidentified glycolipids and two unidentified phospholipids. The respiratory quinone was identified as ubiquinone Q-10. The genomic DNA G+C content was determined to be 56.3 mol%. On the basis of genotypic, chemotaxonomic and phenotypic distinctness, we propose a novel genus, Emcibacter gen. nov., with Emcibacter nanhaiensis sp. nov. as the type species. The type strain is HTCJW17(T) (=CGMCC 1.12471(T) = LMG 27419(T) = MCCC 1A06723(T)). PMID:25577204

Liu, Xiupian; Li, Guangyu; Lai, Qiliang; Sun, Fengqin; Du, Yaping; Shao, Zongze

2015-04-01

349

Proteiniborus ethanoligenes gen. nov., sp. nov., an anaerobic protein-utilizing bacterium.  

PubMed

A novel anaerobic, mesophilic, protein-utilizing bacterial strain, GW(T), was isolated from the mesophilic hydrogen-producing granular sludge used to treat food industry wastewater. The strain was a Gram-positive, non-spore-forming and non-motile rod. Growth of the strain was observed at 20-48 degrees C and at pH 6.4-10.0. The strain used yeast extract and peptone as carbon and energy sources. Weak growth was also observed with tryptone and Casamino acids as carbon and energy sources. The strain used none of the tested carbohydrates, alcohols or fatty acids. The fermentation products in peptone-yeast broth included ethanol, acetic acid, hydrogen and carbon dioxide. Gelatin was not hydrolysed. Nitrate was reduced. Indole was produced. NH(3) and H(2)S were not produced. The DNA G+C content of strain GW(T) was 38.0 mol%. The predominant cellular fatty acids were the saturated fatty acids C(14:0) (15.58%), C(16:0) (25.40%) and C(18:0) (12.03%). Phylogenetic analysis based on 16S rRNA gene sequence similarity revealed that strain GW(T) represented a new branch within cluster XII of the Clostridium subphylum, with <89.6% 16S rRNA gene sequence similarities to all described species. On the basis of polyphasic evidence from this study, strain GW(T) represents a new genus and novel species, for which the name Proteiniborus ethanoligenes gen. nov., sp. nov. is proposed. The type strain is GW(T) (=CGMCC 1.5055(T)=JCM 14574(T)). PMID:18175674

Niu, Lili; Song, Lei; Dong, Xiuzhu

2008-01-01

350

Petrimonas sulfuriphila gen. nov., sp. nov., a mesophilic fermentative bacterium isolated from a biodegraded oil reservoir.  

PubMed

A mesophilic, anaerobic, fermentative bacterium, strain BN3(T), was isolated from a producing well of a biodegraded oil reservoir in Canada. Cells were Gram-negative, non-motile rods that did not form spores. The temperature range for growth was 15-40 degrees C, with optimum growth at 37-40 degrees C. The strain grew with up 4 % NaCl, with optimum growth in the absence of NaCl. Tryptone was required for growth. Yeast extract and elemental sulfur stimulated growth. Growth was also enhanced during fermentation of glucose, arabinose, galactose, maltose, mannose, rhamnose, lactose, ribose, fructose, sucrose, cellobiose, lactate, mannitol and glycerol. Acetate, hydrogen and CO(2) were produced during glucose fermentation. Elemental sulfur and nitrate were used as electron acceptors and were reduced to sulfide and ammonium, respectively. The G + C content of the genomic DNA was 40.8 mol%. Phylogenetic analyses of the 16S rRNA gene sequence indicated that the strain was a member of the phylum 'Bacteroidetes', distantly related to the genera Bacteroides and Tannerella (similarity values of less than 90 %). The chemotaxonomic data (fatty acids, polar lipids and quinones composition) also indicated that strain BN3(T) could be clearly distinguished from its closest cultivated relatives. This novel organism possesses phenotypic, chemotaxonomic and phylogenetic traits that do not allow its classification as a member of any previously described genus; therefore, it is proposed that this isolate should be described as a member of a novel species of a new genus, Petrimonas gen. nov., of which Petrimonas sulfuriphila sp. nov. is the type species. The type strain is BN3(T) (= DSM 16547(T) = JCM 12565(T)). PMID:15879242

Grabowski, Agnès; Tindall, Brian J; Bardin, Véronique; Blanchet, Denis; Jeanthon, Christian

2005-05-01

351

Analysis of first proximity voltage on the property of GEN ? image intensifier  

NASA Astrophysics Data System (ADS)

First proximity voltage is the voltage between the cathode of Low Light Level image intensifier and the input surface of Micro-channel plate?MCP?. There are so many factors influencing the image intensifier performance, and the first proximity voltage is one of the most important factors that can not be ignored. Based on the theory analysis and test of different proximity voltage on the gain?signal-to-noise ratio and equivalent background noise, this test has studied on the important performance of Gen III image intensifier effected by the proximity voltage. By the experimental study, the increase of first proximity voltage to a certain extent can improve gain?signal-to-noise ratio and equivalent background noise at the same time. The main cause of this phenomenon is that the increase of proximity voltage can enlarge the incident electron energy, and then improve the quantum efficiency of the incident electron; meantime, stray electron produced by field emission at the action of the electric field of filmed-MCP will lead to equivalent background deterioration. Ultimately we conclude that: 1) Signal to noise is proportional to the square of he cathode sensitivity, increases with the first collision energy of the incident electron, especially at 200-500ev. 2)In the increasing process of voltage from 300v to 800v, the gain of filmed-MCP increases rapidly, but lower again when Upk increases further because of gain self-saturation; lgG and lgUpk are linear relationship, thus the curve can intuitively demonstrate the relationship between them. 3) Stray electron produced by field emission at the action of the electric field of filmed-MCP will lead to equivalent background deterioration, but will not exceed the requirements of technical specifications?2.5×10-7lx?.

Zhang, Ni; Zhu, Yu-feng; Li, Dan; Nie, Jing; Zhang, Tai-min; Liu, Xiao-jian; Liu, Zhao-lu; Cheng, Wei; Fu, Ling-yun

2013-08-01

352

Halotia gen. nov., a phylogenetically and physiologically coherent cyanobacterial genus isolated from marine coastal environments.  

PubMed

Nostoc is a common and well-studied genus of cyanobacteria and, according to molecular phylogeny, is a polyphyletic group. Therefore, revisions of this genus are urged in an attempt to clarify its taxonomy. Novel strains isolated from underexplored environments and assigned morphologically to the genus Nostoc are not genetically related to the 'true Nostoc' group. In this study, four strains isolated from biofilms collected in Antarctica and five strains originated from Brazilian mangroves were evaluated. Despite their morphological similarities to other morphotypes of Nostoc, these nine strains differed from other morphotypes in ecological, physiological and genetic aspects. Based on the phylogeny of the 16S rRNA gene, the Antarctic sequences were grouped together with the sequences of the Brazilian mangrove isolates and Nostoc sp. Mollenhauer 1?:?1-067 in a well-supported cluster (74?% bootstrap value, maximum-likelihood). This novel cluster was separated phylogenetically from the 'true Nostoc' clade and from the clades of the morphologically similar genera Mojavia and Desmonostoc. The 16S rRNA gene sequences generated in this study exhibited 96?% similarity to sequences from the nostocacean genera mentioned above. Physiologically, these nine strains showed the capacity to grow in a salinity range of 1-10?% NaCl, indicating their tolerance of saline conditions. These results provide support for the description of a new genus, named Halotia gen. nov., which is related morphologically to the genera Nostoc, Mojavia and Desmonostoc. Within this new genus, three novel species were recognized and described based on morphology and internal transcribed spacer secondary structures: Halotia branconii sp. nov., Halotia longispora sp. nov. and Halotia wernerae sp. nov., under the provisions of the International Code of Nomenclature for Algae, Fungi and Plants. PMID:25424485

Genuário, Diego Bonaldo; Vaz, Marcelo Gomes Marçal Vieira; Hentschke, Guilherme Scotta; Sant'Anna, Célia Leite; Fiore, Marli Fátima

2015-02-01

353

Brockia lithotrophica gen. nov., sp. nov., an anaerobic thermophilic bacterium from a terrestrial hot spring.  

PubMed

A novel thermophilic bacterium, strain Kam1851(T), was isolated from a terrestrial hot spring of the Uzon Caldera, Kamchatka Peninsula, Russia. Cells of strain Kam1851(T) were spore-forming rods with a gram-positive type of cell wall. Growth was observed between 46 and 78 °C, and pH 5.5-8.5. The optimal growth (doubling time, 6.0 h) was at 60-65 °C and pH 6.5. The isolate was an obligate anaerobe growing in pre-reduced medium only. It grew on mineral medium with molecular hydrogen or formate as electron donors, and elemental sulfur, thiosulfate or polysulfide as electron acceptors. The main cellular fatty acids were C(16?:?0) (34.2?%), iso-C(16?:?0) (18?%), C(18?:?0) (12.8?%) and iso-C(17?:?0) (11.1?%). The G+C content of the genomic DNA of strain Kam1851(T) was 63 mol%. 16S rRNA gene sequence analysis showed that strain Kam1851(T) belonged to the order Thermoanaerobacterales, but it was not closely related to representatives of any genera with validly published names. The most closely related strains, which had no more than 89.2?% sequence similarity, were members of the genera Ammonifex and Caldanaerobacter. On the basis of its phylogenetic position and novel phenotypic features, isolate Kam1851(T) is proposed to represent a novel species in a new genus, Brockia lithotrophica gen. nov., sp. nov.; the type strain of Brockia lithotrophica is Kam1851(T) (?=?DSM 22653(T)?=?VKM B-2685(T)). PMID:22493174

Perevalova, Anna A; Kublanov, Ilya V; Baslerov, R V; Zhang, Gengxin; Bonch-Osmolovskaya, Elizaveta A

2013-02-01

354

Stomatobaculum longum gen. nov., sp. nov., an obligately anaerobic bacterium from the human oral cavity  

PubMed Central

A strictly anaerobic Gram-stain-variable but positive by structure, non-spore-forming bacterium designated Lachnospiraceae bacterium ACC2 strain DSM 24645T was isolated from human subgingival dental plaque. Bacterial cells were 4–40 µm long non-motile rods, often swollen and forming curved filaments up to 200 µm. Cells contained intracellular, poorly crystalline, nanometre-sized iron- and sulfur-rich particles. The micro-organism was able to grow on yeast extract, trypticase peptone, milk, some sugars and organic acids. The major metabolic end-products of glucose fermentation were butyrate, lactate, isovalerate and acetate. The growth temperature and pH ranges were 30–42 °C and 4.9–7.5, respectively. Major fatty acids were C14?:?0, C14?:?0 DMA (dimethyl aldehyde), C16?:?0, C16?:?1?7c DMA. The whole-cell hydrolysate contained meso-diaminopimelic acid, indicating peptidoglycan type A1?. The DNA G+C content was calculated to be 55.05 mol% from the whole-genome sequence and 55.3 mol% as determined by HPLC. There were no predicted genes responsible for biosynthesis of respiratory lipoquinones, mycolic acids and lipopolysaccharides. Genes associated with synthesis of teichoic and lipoteichoic acids, diaminopimelic acid, polar lipids and polyamines were present. According to the 16S rRNA gene sequence phylogeny, strain DSM 24645T formed, together with several uncultured oral clones, a separate branch within the family Lachnospiraceae, with the highest sequence similarity to the type strain of Moryella indoligenes at 94.2?%. Based on distinct phenotypic and genotypic characteristics, we suggest that strain DSM 24645T represents a novel species in a new genus, for which the name Stomatobaculum longum gen. nov., sp. nov. is proposed. The type strain of Stomatobaculum longum is DSM 24645T (?=?HM-480T; deposited in BEI Resources, an NIH collection managed by the ATCC). PMID:22843721

Muller, Paul; Panikov, Nicolai; Mandalakis, Manolis; Hohmann, Tine; Hazen, Amanda; Fowle, William; Prozorov, Tanya; Bazylinski, Dennis A.

2013-01-01

355

Geothermomicrobium terrae gen. nov., sp. nov., a novel member of the family Thermoactinomycetaceae.  

PubMed

Strains YIM 77562(T) and YIM 77580, two novel Gram-staining-positive, filamentous bacterial isolates, were recovered from the Rehai geothermal field, Tengchong, Yunnan province, south-west China. Good growth was observed at 50-55 °C and pH 7.0. Aerial mycelium was absent on all media tested. Substrate mycelium was well-developed, long and moderately flexuous, and formed abundant, single, warty, ornamented endospores. Phylogenetic analysis of the 16S rRNA gene sequences of the two strains indicated that they belong to the family Thermoactinomycetaceae. Similarity levels between the 16S rRNA gene sequences of the two strains and those of type strains of members of the Thermoactinomycetaceae were 88.33-93.24?%; the highest sequence similarity was with Hazenella coriacea DSM 45707(T). In both strains, the predominant menaquinone was MK-7, the diagnostic diamino acid was meso-diaminopimelic acid and the major cellular fatty acids were iso-C14?:?0, iso-C15?:?0 and iso-C16?:?0. The major polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, unidentified polar lipids and unidentified phospholipids. The genomic DNA G+C contents of strains YIM 77562(T) and YIM 77580 were 45.5 and 44.2 mol%, respectively. DNA-DNA relatedness data suggest that the two isolates represent a single species. Based on phylogenetic analyses and physiological and biochemical characteristics, it is proposed that the two strains represent a single novel species in a new genus, Geothermomicrobium terrae gen. nov., sp. nov. The type strain of Geothermomicrobium terrae is YIM 77562(T) (?=?CCTCC AA 2011022(T)?=?JCM 18057(T)). PMID:24907264

Zhou, En-Min; Yu, Tian-Tian; Liu, Lan; Ming, Hong; Yin, Yi-Rui; Dong, Lei; Tseng, Min; Nie, Guo-Xing; Li, Wen-Jun

2014-09-01

356

Cnuella takakiae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from Takakia lepidozioides.  

PubMed

A Gram-staining-negative, rod-shaped and non-spore-forming bacterium, designated strain RG1-1(T), was isolated from Takakia lepidozioides collected from Gawalong glacier in Tibet, China, and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain RG1-1(T) were iso-C(15 : 0) (19.8%), summed feature 3 (C(16 : 1)?7c and/or C(16 : 1)?6c, 17.0%), C(16 : 0 (9.9)%) and iso-C(17 : 0) 3-OH (9.4%); its major polar lipids were phosphatidylethanolamine, four unidentified aminolipids, one unidentified phospholipid, one unidentified aminoglycolipid, one unidentified glycolipid, and three unidentified lipids. Strain RG1-1(T) contained MK-7 as the dominant menaquinone, and the G+C content of its genomic DNA was 49.1 mol%. Strain RG1-1(T) exhibited the highest 16S rRNA gene sequence similarity (91.8%) with Flavisolibacter ginsengiterrae Gsoil 492(T) and Flavisolibacter ginsengisoli Gsoil 643(T). Phylogenetic analysis showed that strain RG1-1(T) was a member of the family Chitinophagaceae, phylum Bacteroidetes. On the basis of 16S rRNA gene sequence analysis, and phenotypic and chemotaxonomic data, strain RG1-1(T) is considered to represent a novel species of a novel genus, for which the name Cnuella takakiae gen. nov., sp. nov. is proposed. The type strain is RG1-1(T) (?=?CGMCC 1.12492(T)?=?DSM 26897(T)). PMID:24198053

Zhao, Ran; Chen, Xin Yao; Li, Xue Dong; Tian, Yang; Kong, Bi He; Chen, Zhi Ling; Li, Yan Hong

2014-02-01

357

Association of Comorbidity Burden With Abnormal Cardiac Mechanics: Findings From the HyperGEN Study  

PubMed Central

Background Comorbidities are common in heart failure (HF), and the number of comorbidities has been associated with poor outcomes in HF patients. However, little is known about the effect of multiple comorbidities on cardiac mechanics, which could impact the pathogenesis of HF. We sought to determine the relationship between comorbidity burden and adverse cardiac mechanics. Methods and Results We performed speckle?tracking analysis on echocardiograms from the HyperGEN study (n=2150). Global longitudinal, circumferential, and radial strain, and early diastolic (e') tissue velocities were measured. We evaluated the association between comorbidity number and cardiac mechanics using linear mixed effects models to account for relatedness among subjects. The mean age was 51±14 years, 58% were female, and 47% were African American. Dyslipidemia and hypertension were the most common comorbidities (61% and 58%, respectively). After adjusting for left ventricular (LV) mass index, ejection fraction, and several potential confounders, the number of comorbidities remained associated with all indices of cardiac mechanics except global circumferential strain (eg, ?=?0.32 [95% CI ?0.44, ?0.20] per 1?unit increase in number of comorbidities for global longitudinal strain; ?=?0.16 [95% CI ?0.20, ?0.11] for e' velocity; P?0.0001 for both comparisons). Results were similar after excluding participants with abnormal LV geometry (P<0.05 for all comparisons). Conclusions Higher comorbidity burden is associated with worse cardiac mechanics, even in the presence of normal LV geometry. The deleterious effect of multiple comorbidities on cardiac mechanics may explain both the high comorbidity burden and adverse outcomes in patients who ultimately develop HF. PMID:24780206

Selvaraj, Senthil; Aguilar, Frank G.; Martinez, Eva E.; Beussink, Lauren; Kim, Kwang?Youn A.; Peng, Jie; Rasmussen?Torvik, Laura; Sha, Jin; Irvin, Marguerite R.; Gu, C. Charles; Lewis, Cora E.; Hunt, Steven C.; Arnett, Donna K.; Shah, Sanjiv J.

2014-01-01

358

Tropicihabitans flavus gen. nov., sp. nov., a new member of the family Cellulomonadaceae.  

PubMed

Two novel Gram-stain positive actinobacteria, designated PS-14-16(T) and RS-7-1, were isolated from the rhizosphere of a mangrove and sea sediment, respectively, and their taxonomic positions were investigated by a polyphasic approach. Both strains were observed to form vegetative hyphae in the early phase of growth but the hyphae eventually fragment into short rods to coccoid cells. The peptidoglycan type of both strains was found to be A4?. Their predominant menaquinone was identified as MK-9(H4) and the major fatty acid as anteiso-C15:0. The DNA G+C content was determined to be 68.4-68.5 mol%. 16S rRNA gene sequencing revealed that strains PS-14-16(T) and RS-7-1 were related to members of the family Cellulomonadaceae. Their nearest phylogenetic neighbour was found to be Sediminihabitans luteus, which is currently the only species of the genus Sediminihabitans, with a similarity of 97.94 %. However, strains PS-14-16(T) and RS-7-1 were distinguishable from the members of the genus Sediminihabitans and the other genera within the family Cellulomonadaceae in terms of chemotaxonomic characteristics and phylogenetic relationship. The results of DNA-DNA hybridization experiments indicated that strains PS-14-16(T) and RS-7-1 belong to the same species. Strains PS-14-16(T) and RS-7-1 are concluded to represent a novel genus and species of the family Cellulomonadaceae, for which the name Tropicihabitans flavus gen. nov., sp. nov. is proposed. The type strain of T. flavus is PS-14-16(T) (=NBRC 110109(T) = IanCC A 516(T)). PMID:25761861

Hamada, Moriyuki; Shibata, Chiyo; Nurkanto, Arif; Ratnakomala, Shanti; Lisdiyanti, Puspita; Tamura, Tomohiko; Suzuki, Ken-Ichiro

2015-05-01

359

Rhodoluna lacicola gen. nov., sp. nov., a planktonic freshwater bacterium with stream-lined genome  

PubMed Central

A pure culture of an actinobacterium previously described as ‘Candidatus Rhodoluna lacicola’ strain MWH-Ta8 was established and deposited in two public culture collections. Strain MWH-Ta8T represents a free-living planktonic freshwater bacterium obtained from hypertrophic Meiliang Bay, Lake Taihu, PR China. The strain was characterized by phylogenetic and taxonomic investigations, as well as by determination of its complete genome sequence. Strain MWH-Ta8T is noticeable due to its unusually low values of cell size (0.05 µm3), genome size (1.43 Mbp), and DNA G+C content (51.5 mol%). Phylogenetic analyses based on 16S rRNA gene and RpoB sequences suggested that strain MWH-Ta8T is affiliated with the family Microbacteriaceae with Pontimonas salivibrio being its closest relative among the currently described species within this family. Strain MWH-Ta8T and the type strain of Pontimonas salivibrio shared a 16S rRNA gene sequence similarity of 94.3?%. The cell-wall peptidoglycan of strain MWH-Ta8T was of type B2? (B10), containing 2,4-diaminobutyric acid as the diamino acid. The predominant cellular fatty acids were anteiso-C15?:?0 (36.5?%), iso-C16?:?0 (16.5?%), iso-C15?:?0 (15.6?%) and iso-C14?:?0 (8.9?%), and the major (>10?%) menaquinones were MK-11 and MK-12. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The combined phylogenetic, phenotypic and chemotaxonomic data clearly suggest that strain MWH-Ta8T represents a novel species of a new genus in the family Microbacteriaceae, for which the name Rhodoluna lacicola gen. nov., sp. nov. is proposed. The type strain of the type species is MWH-Ta8T (?=?DSM 23834T?=?LMG 26932T). PMID:24984700

Schmidt, Johanna; Taipale, Sami J.; Doolittle, W. Ford; Koll, Ulrike

2014-01-01

360

Rhodoluna lacicola gen. nov., sp. nov., a planktonic freshwater bacterium with stream-lined genome.  

PubMed

A pure culture of an actinobacterium previously described as 'Candidatus Rhodoluna lacicola' strain MWH-Ta8 was established and deposited in two public culture collections. Strain MWH-Ta8(T) represents a free-living planktonic freshwater bacterium obtained from hypertrophic Meiliang Bay, Lake Taihu, PR China. The strain was characterized by phylogenetic and taxonomic investigations, as well as by determination of its complete genome sequence. Strain MWH-Ta8(T) is noticeable due to its unusually low values of cell size (0.05 µm(3)), genome size (1.43 Mbp), and DNA G+C content (51.5 mol%). Phylogenetic analyses based on 16S rRNA gene and RpoB sequences suggested that strain MWH-Ta8(T) is affiliated with the family Microbacteriaceae with Pontimonas salivibrio being its closest relative among the currently described species within this family. Strain MWH-Ta8(T) and the type strain of Pontimonas salivibrio shared a 16S rRNA gene sequence similarity of 94.3?%. The cell-wall peptidoglycan of strain MWH-Ta8(T) was of type B2? (B10), containing 2,4-diaminobutyric acid as the diamino acid. The predominant cellular fatty acids were anteiso-C15?:?0 (36.5?%), iso-C16?:?0 (16.5?%), iso-C15?:?0 (15.6?%) and iso-C14?:?0 (8.9?%), and the major (>10?%) menaquinones were MK-11 and MK-12. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The combined phylogenetic, phenotypic and chemotaxonomic data clearly suggest that strain MWH-Ta8(T) represents a novel species of a new genus in the family Microbacteriaceae, for which the name Rhodoluna lacicola gen. nov., sp. nov. is proposed. The type strain of the type species is MWH-Ta8(T) (?=?DSM 23834(T)?=?LMG 26932(T)). PMID:24984700

Hahn, Martin W; Schmidt, Johanna; Taipale, Sami J; Doolittle, W Ford; Koll, Ulrike

2014-09-01

361

Magnetovibrio blakemorei gen. nov., sp. nov., a magnetotactic bacterium (Alphaproteobacteria: Rhodospirillaceae) isolated from a salt marsh.  

PubMed

A magnetotactic bacterium, designated strain MV-1(T), was isolated from sulfide-rich sediments in a salt marsh near Boston, MA, USA. Cells of strain MV-1(T) were Gram-negative, and vibrioid to helicoid in morphology. Cells were motile by means of a single polar flagellum. The cells appeared to display a transitional state between axial and polar magnetotaxis: cells swam in both directions, but generally had longer excursions in one direction than the other. Cells possessed a single chain of magnetosomes containing truncated hexaoctahedral crystals of magnetite, positioned along the long axis of the cell. Strain MV-1(T) was a microaerophile that was also capable of anaerobic growth on some nitrogen oxides. Salinities greater than 10?% seawater were required for growth. Strain MV-1(T) exhibited chemolithoautotrophic growth on thiosulfate and sulfide with oxygen as the terminal electron acceptor (microaerobic growth) and on thiosulfate using nitrous oxide (N2O) as the terminal electron acceptor (anaerobic growth). Chemo-organoautotrophic and methylotrophic growth was supported by formate under microaerobic conditions. Autotrophic growth occurred via the Calvin-Benson-Bassham cycle. Chemo-organoheterotrophic growth was supported by various organic acids and amino acids, under microaerobic and anaerobic conditions. Optimal growth occurred at pH 7.0 and 26-28 °C. The genome of strain MV-1(T) consisted of a single, circular chromosome, about 3.7 Mb in size, with a G+C content of 52.9-53.5 mol%.Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MV-1(T) belongs to the family Rhodospirillaceae within the Alphaproteobacteria, but is not closely related to the genus Magnetospirillum. The name Magnetovibrio blakemorei gen. nov., sp. nov. is proposed for strain MV-1(T). The type strain of Magnetovibrio blakemorei is MV-1(T) (?=?ATCC BAA-1436(T) ?=?DSM 18854(T)). PMID:22984137

Bazylinski, Dennis A; Williams, Timothy J; Lefèvre, Christopher T; Trubitsyn, Denis; Fang, Jiasong; Beveridge, Terrence J; Moskowitz, Bruce M; Ward, Bruce; Schübbe, Sabrina; Dubbels, Bradley L; Simpson, Brian

2013-05-01

362

High quality draft genome sequence and description of Occidentia massiliensis gen. nov., sp. nov., a new member of the family Rickettsiaceae  

PubMed Central

The family Rickettsiaceae currently includes two genera: Orientia that contains one species, Orientiatsutsugamushi, and Rickettsia that contains 28 species. Occidentia massiliensis gen. nov., sp. nov. strain OS118T is the type strain of O. massiliensis gen. nov., sp. nov., the type species of the new genus Occidentia gen. nov. within the family Rickettsiaceae. This strain, whose genome is described here, was isolated in France from the soft tick Ornithodoros sonrai collected in Senegal. O. massiliensis is an aerobic, rod-shaped, Gram-negative, obligate intracellular bacillus that may be cultivated in BME/CTVM2 cells. Here we describe the features of O. massiliensis, together with the complete genomic sequencing and annotation. The 1,469,252 bp long genome (1 chromosome but no plasmid) contains 1,670 protein-coding and 41 RNA genes, including one rRNA operon.

2014-01-01

363

WallGen, Software to Construct Layered Cellulose-Hemicellulose Networks and Predict Their Small Deformation Mechanics1  

PubMed Central

We understand few details about how the arrangement and interactions of cell wall polymers produce the mechanical properties of primary cell walls. Consequently, we cannot quantitatively assess if proposed wall structures are mechanically reasonable or assess the effectiveness of proposed mechanisms to change mechanical properties. As a step to remedying this, we developed WallGen, a Fortran program (available on request) building virtual cellulose-hemicellulose networks by stochastic self-assembly whose mechanical properties can be predicted by finite element analysis. The thousands of mechanical elements in the virtual wall are intended to have one-to-one spatial and mechanical correspondence with their real wall counterparts of cellulose microfibrils and hemicellulose chains. User-defined inputs set the properties of the two polymer types (elastic moduli, dimensions of microfibrils and hemicellulose chains, hemicellulose molecular weight) and their population properties (microfibril alignment and volume fraction, polymer weight percentages in the network). This allows exploration of the mechanical consequences of variations in nanostructure that might occur in vivo and provides estimates of how uncertainties regarding certain inputs will affect WallGen's mechanical predictions. We summarize WallGen's operation and the choice of values for user-defined inputs and show that predicted values for the elastic moduli of multinet walls subject to small displacements overlap measured values. “Design of experiment” methods provide systematic exploration of how changed input values affect mechanical properties and suggest that changing microfibril orientation and/or the number of hemicellulose cross-bridges could change wall mechanical anisotropy. PMID:20007450

Kha, Hung; Tuble, Sigrid C.; Kalyanasundaram, Shankar; Williamson, Richard E.

2010-01-01

364

WallGen, software to construct layered cellulose-hemicellulose networks and predict their small deformation mechanics.  

PubMed

We understand few details about how the arrangement and interactions of cell wall polymers produce the mechanical properties of primary cell walls. Consequently, we cannot quantitatively assess if proposed wall structures are mechanically reasonable or assess the effectiveness of proposed mechanisms to change mechanical properties. As a step to remedying this, we developed WallGen, a Fortran program (available on request) building virtual cellulose-hemicellulose networks by stochastic self-assembly whose mechanical properties can be predicted by finite element analysis. The thousands of mechanical elements in the virtual wall are intended to have one-to-one spatial and mechanical correspondence with their real wall counterparts of cellulose microfibrils and hemicellulose chains. User-defined inputs set the properties of the two polymer types (elastic moduli, dimensions of microfibrils and hemicellulose chains, hemicellulose molecular weight) and their population properties (microfibril alignment and volume fraction, polymer weight percentages in the network). This allows exploration of the mechanical consequences of variations in nanostructure that might occur in vivo and provides estimates of how uncertainties regarding certain inputs will affect WallGen's mechanical predictions. We summarize WallGen's operation and the choice of values for user-defined inputs and show that predicted values for the elastic moduli of multinet walls subject to small displacements overlap measured values. "Design of experiment" methods provide systematic exploration of how changed input values affect mechanical properties and suggest that changing microfibril orientation and/or the number of hemicellulose cross-bridges could change wall mechanical anisotropy. PMID:20007450

Kha, Hung; Tuble, Sigrid C; Kalyanasundaram, Shankar; Williamson, Richard E

2010-02-01

365

Cooperative Ge-N Bond activation in aluminium-functionalised aminogermanes and spontaneous imine elimination via an intermediate germyl cation.  

PubMed

Hydrometallation of iPr2 N-Ge(CMe3 )(C?C-CMe3 )2 with H-M(CMe3 )2 (M=Al, Ga) affords alkenyl-alkynylgermanes in which the Lewis-acidic metal atoms are not coordinated by the amino N atoms but by the ?-C atoms of the ethynyl groups. These interactions result in a lengthening of the Ge-C bonds by approximately 10?pm and a comparably strong deviation of the Ge-C?C angle from linearity (154.3(1)°). This unusual behaviour may be caused by steric shielding of the N atoms. Coordination of the metal atoms by the amino groups is observed upon hydrometallation of Et2 N-Ge(C6 H5 )(C?C-CMe3 )2 , bearing a smaller NR2 group. Strong M-N interactions lead to a lengthening of the Ge-N bonds by 10 to 15?pm and a strong deviation of the M atoms from the MC3 plane by 52 and 47?pm, for Al and Ga, respectively. Dual hydrometallation is achieved only with HAl(CMe3 )2 . In the product, there is a strong Al-N bond with converging Al-N and Ge-N distances (208 vs. 200?pm) and an interaction of the second Al atom to the phenyl group. Addition of chloride anions terminates the latter interaction while the activated Ge-N bond undergoes an unprecedented elimination of EtN=C(H)Me at room temperature, leading to a germane with a Ge-H bond. State-of-the-art DFT calculations reveal that the unique mechanism comprises the transfer of the amino group from Ge to Al to yield an intermediate germyl cation as a strong Lewis acid, which induces ?-hydride elimination, with chloride binding being crucial for providing the thermodynamic driving force. PMID:25521391

Uhl, Werner; Tannert, Jens; Honacker, Christian; Layh, Marcus; Qu, Zheng-Wang; Risthaus, Tobias; Grimme, Stefan

2015-02-01

366

Sumatrella chelonica gen. n., sp. n., a new remarkable genus and species from Indonesia, Sumatra (Acari, Uropodina, Oplitidae)  

PubMed Central

Abstract A new genus Sumatrella gen. n. is described and illustrated based on the new species Sumatrella chelonica sp. n. collected in Sumatra, Indonesia. The new genus belongs to the family Oplitidae based on its hypertrichous internal malae and the absence of strongly sclerotized structures on the dorsal shield. The new genus is closely related to the genus Chelonuropoda Sellnick, 1954 but the transverse furrow on ventral idiosoma close to coxae IV and the strongly sclerotized C-shaped dorsal line are missing in the new genus. These characters can be found in species of Chelonuropoda.

Kontschán, Jen?

2015-01-01

367

Accuracy evaluation of ClimGen weather generator and daily to hourly disaggregation methods in tropical conditions  

NASA Astrophysics Data System (ADS)

Daily and sub-daily weather data are often required for hydrological and environmental modeling. Various weather generator programs have been used to generate synthetic climate data where observed climate data are limited. In this study, a weather data generator, ClimGen, was evaluated for generating information on daily precipitation, temperature, and wind speed at four tropical watersheds located in Hawai`i, USA. We also evaluated different daily to sub-daily weather data disaggregation methods for precipitation, air temperature, dew point temperature, and wind speed at M?kaha watershed. The hydrologic significance values of the different disaggregation methods were evaluated using Distributed Hydrology Soil Vegetation Model. MuDRain and diurnal method performed well over uniform distribution in disaggregating daily precipitation. However, the diurnal method is more consistent if accurate estimates of hourly precipitation intensities are desired. All of the air temperature disaggregation methods performed reasonably well, but goodness-of-fit statistics were slightly better for sine curve model with 2 h lag. Cosine model performed better than random model in disaggregating daily wind speed. The largest differences in annual water balance were related to wind speed followed by precipitation and dew point temperature. Simulated hourly streamflow, evapotranspiration, and groundwater recharge were less sensitive to the method of disaggregating daily air temperature. ClimGen performed well in generating the minimum and maximum temperature and wind speed. However, for precipitation, it clearly underestimated the number of extreme rainfall events with an intensity of >100 mm/day in all four locations. ClimGen was unable to replicate the distribution of observed precipitation at three locations (Honolulu, Kahului, and Hilo). ClimGen was able to reproduce the distributions of observed minimum temperature at Kahului and wind speed at Kahului and Hilo. Although the weather data generation and disaggregation methods were concentrated in a few Hawaiian watersheds, the results presented can be used to similar mountainous location settings, as well as any specific locations aimed at furthering the site-specific performance evaluation of these tested models.

Safeeq, Mohammad; Fares, Ali

2011-12-01

368

GIDL: a rule based expert system for GenBank Intelligent Data Loading into the Molecular Biodiversity database  

PubMed Central

Background In the scientific biodiversity community, it is increasingly perceived the need to build a bridge between molecular and traditional biodiversity studies. We believe that the information technology could have a preeminent role in integrating the information generated by these studies with the large amount of molecular data we can find in bioinformatics public databases. This work is primarily aimed at building a bioinformatic infrastructure for the integration of public and private biodiversity data through the development of GIDL, an Intelligent Data Loader coupled with the Molecular Biodiversity Database. The system presented here organizes in an ontological way and locally stores the sequence and annotation data contained in the GenBank primary database. Methods The GIDL architecture consists of a relational database and of an intelligent data loader software. The relational database schema is designed to manage biodiversity information (Molecular Biodiversity Database) and it is organized in four areas: MolecularData, Experiment, Collection and Taxonomy. The MolecularData area is inspired to an established standard in Generic Model Organism Databases, the Chado relational schema. The peculiarity of Chado, and also its strength, is the adoption of an ontological schema which makes use of the Sequence Ontology. The Intelligent Data Loader (IDL) component of GIDL is an Extract, Transform and Load software able to parse data, to discover hidden information in the GenBank entries and to populate the Molecular Biodiversity Database. The IDL is composed by three main modules: the Parser, able to parse GenBank flat files; the Reasoner, which automatically builds CLIPS facts mapping the biological knowledge expressed by the Sequence Ontology; the DBFiller, which translates the CLIPS facts into ordered SQL statements used to populate the database. In GIDL Semantic Web technologies have been adopted due to their advantages in data representation, integration and processing. Results and conclusions Entries coming from Virus (814,122), Plant (1,365,360) and Invertebrate (959,065) divisions of GenBank rel.180 have been loaded in the Molecular Biodiversity Database by GIDL. Our system, combining the Sequence Ontology and the Chado schema, allows a more powerful query expressiveness compared with the most commonly used sequence retrieval systems like Entrez or SRS. PMID:22536971

2012-01-01

369

Practical aspects to realize data transfer between a patient administration mastersystem and clinical subsystems using ProtoGen/HL7.  

PubMed

At the Medizinische Einrichtungen Bonn the module IS-H of SAP R/3 is used for patient administration. All clinical subsystems will be provided with patient data using HL7 2.3 messages. These messages are generated from HCM messages a proprietary format of IS-H by means of a communication server. Interface application for subsystems which are not capable of processing HL7 messages are written using the open source C++ class library ProtoGen/HL7. First experiences and remaining problems are discussed. PMID:11187602

Jung, B; Goldschmidt, A; Langenberg, S

2000-01-01

370

Modeling and Evaluating Pilot Performance in NextGen: Review of and Recommendations Regarding Pilot Modeling Efforts, Architectures, and Validation Studies  

NASA Technical Reports Server (NTRS)

NextGen operations are associated with a variety of changes to the national airspace system (NAS) including changes to the allocation of roles and responsibilities among operators and automation, the use of new technologies and automation, additional information presented on the flight deck, and the entire concept of operations (ConOps). In the transition to NextGen airspace, aviation and air operations designers need to consider the implications of design or system changes on human performance and the potential for error. To ensure continued safety of the NAS, it will be necessary for researchers to evaluate design concepts and potential NextGen scenarios well before implementation. One approach for such evaluations is through human performance modeling. Human performance models (HPMs) provide effective tools for predicting and evaluating operator performance in systems. HPMs offer significant advantages over empirical, human-in-the-loop testing in that (1) they allow detailed analyses of systems that have not yet been built, (2) they offer great flexibility for extensive data collection, (3) they do not require experimental participants, and thus can offer cost and time savings. HPMs differ in their ability to predict performance and safety with NextGen procedures, equipment and ConOps. Models also vary in terms of how they approach human performance (e.g., some focus on cognitive processing, others focus on discrete tasks performed by a human, while others consider perceptual processes), and in terms of their associated validation efforts. The objectives of this research effort were to support the Federal Aviation Administration (FAA) in identifying HPMs that are appropriate for predicting pilot performance in NextGen operations, to provide guidance on how to evaluate the quality of different models, and to identify gaps in pilot performance modeling research, that could guide future research opportunities. This research effort is intended to help the FAA evaluate pilot modeling efforts and select the appropriate tools for future modeling efforts to predict pilot performance in NextGen operations.

Wickens, Christopher; Sebok, Angelia; Keller, John; Peters, Steve; Small, Ronald; Hutchins, Shaun; Algarin, Liana; Gore, Brian Francis; Hooey, Becky Lee; Foyle, David C.

2013-01-01

371

search GenBank: interactive orchestration and ad-hoc choreography of Web services in the exploration of the biomedical resources of the National Center For Biotechnology Information  

PubMed Central

Background Due to the growing number of biomedical entries in data repositories of the National Center for Biotechnology Information (NCBI), it is difficult to collect, manage and process all of these entries in one place by third-party software developers without significant investment in hardware and software infrastructure, its maintenance and administration. Web services allow development of software applications that integrate in one place the functionality and processing logic of distributed software components, without integrating the components themselves and without integrating the resources to which they have access. This is achieved by appropriate orchestration or choreography of available Web services and their shared functions. After the successful application of Web services in the business sector, this technology can now be used to build composite software tools that are oriented towards biomedical data processing. Results We have developed a new tool for efficient and dynamic data exploration in GenBank and other NCBI databases. A dedicated search GenBank system makes use of NCBI Web services and a package of Entrez Programming Utilities (eUtils) in order to provide extended searching capabilities in NCBI data repositories. In search GenBank users can use one of the three exploration paths: simple data searching based on the specified user’s query, advanced data searching based on the specified user’s query, and advanced data exploration with the use of macros. search GenBank orchestrates calls of particular tools available through the NCBI Web service providing requested functionality, while users interactively browse selected records in search GenBank and traverse between NCBI databases using available links. On the other hand, by building macros in the advanced data exploration mode, users create choreographies of eUtils calls, which can lead to the automatic discovery of related data in the specified databases. Conclusions search GenBank extends standard capabilities of the NCBI Entrez search engine in querying biomedical databases. The possibility of creating and saving macros in the search GenBank is a unique feature and has a great potential. The potential will further grow in the future with the increasing density of networks of relationships between data stored in particular databases. search GenBank is available for public use at http://sgb.biotools.pl/. PMID:23452691

2013-01-01

372

A new genus and species of isanthid sea anemone (Cnidaria: Anthozoa: Actiniaria) from Chilean Patagonia, Anthoparactis fossii n. gen. et sp  

NASA Astrophysics Data System (ADS)

We describe a new genus and species of sea anemone from Chilean Patagonia. Anthoparactis fossii n. gen. et sp. adds another acontiate genus and species to the family Isanthidae Carlgren, 1938. Anthoparactis n. gen. differs from the other isanthid genera in having the same number of mesenteries distally and proximally, acontia with basitrichs only, and a column with verrucae distally. Anthoparactis fossii n. sp. differs from the most similar species, Isoparactis fionae Lauretta et al., 2013, in the number of cycles of mesenteries and tentacles, structures of the column, colour pattern of the oral disc, cnidae, and geographical distribution. Isanthidae now includes seven genera and 11 species.

Häussermann, Verena; Rodríguez, Estefanía

2014-09-01

373

Kilianites gen. nov., Himalayitidae (Ammonitina) nouveau du Berriasien de la région de Cabra (province de Cordoba, SE Espagne): descendance de Protacanthodiscus  

NASA Astrophysics Data System (ADS)

A new ammonite genus of the himalayitids is described for the group of Hoplites bergeroni Kilian, 1889. Kilianites gen. nov. is only known by its macroconchs (M), until now ascribed to Protacanthodiscus, macroconch of Durangites (pars), or/and Neocosmoceras. The relationships with Protacanthodiscus and Neocosmoceras are discussed, taking into account characters of the ontogenesis and stratigraphic position: Kilianites gen. nov., of the Berriasian Euxinus Zone, Jacobi Subzone (= calpionellids B zone) succeeds the Durangites-Protacanthodiscus acme in the Upper Tithonian Durangites Zone (= A2 and A3 calpionellids subzones).

Enay, Raymond; Boughdiri, Mabrouk; Hégarat, Gérard Le

1998-10-01

374

Iranotrichia gen. n., a new genus of Scenopinidae (Diptera) from Iran, with a key to window fly genera of the world  

PubMed Central

Abstract An unusual new genus of Scenopinidae, Iranotrichia gen. n., comprising two newly discovered species (Iranotrichia insolita sp. n. and Iranotrichia nigra sp. n.), is described from Iran. Iranotrichia gen. n. are scenopinine window flies with a habitus reminiscent of certain bee fly genera (Bombyliidae), based on colouration and elongate mouthparts and antennae. The phylogenetic placement of this distinctive new genus is discussed and a dichotomous key to world genera is presented. The genus name Kelseyana nom. n. is proposed to replace Caenoneura Kröber, 1924, which was found to be preoccupied by Thomson (1870: 270) (Hymenoptera) and Kirby (1890: 136) (Odonata). PMID:22144855

Winterton, Shaun L.; Gharali, Babak

2011-01-01

375

Reclassification of the polyphyletic genus Prosthecomicrobium to form two novel genera, Vasilyevaea gen. nov. and Bauldia gen. nov. with four new combinations: Vasilyevaea enhydra comb. nov., Vasilyevaea mishustinii comb. nov., Bauldia consociata comb. nov. and Bauldia litoralis comb. nov.  

PubMed Central

Species of the genus Prosthecomicrobium are noted for their numerous cellular appendages or prosthecae that extend from the cells. This investigation confirms that the genus is polyphyletic based on an extensive analysis of the 16S rRNA gene sequences of several named species of the genus. The analyses indicate that some Prosthecomicrobium species are more closely related to non-prosthecate genera, including Devosia, Labrenzia, Blastochloris, Methylosinus, Mesorhizobium and Kaistia, than they are to other species of the genus Prosthecomicrobium. For this reason, two of the Prosthecomicrobium clades which are polyphyletic with the type species, Prosthecomicrobium pneumaticum, are renamed as new genera. The currently named species Prosthecomicrobium enhydrum, Prosthecomicrobium mishustinii, Prosthecomicrobium consociatum and Prosthecomicrobium litoralum are reclassified in two new genera, Vasilyevaea gen. nov. and Bauldia gen. nov. with four new combinations: Vasilyevaea enhydra comb. nov. (the type species) and Vasilyevaea mishustinii comb. nov., and Bauldia consociata comb. nov. and Bauldia litoralis comb. nov. (the type species). The type strain of Vasilyevaea enhydra is strain 9bT (=ATCC 23634T =VKM B-1376T). The type strain of the other species in this genus is Vasilyevaea mishustinii strain 17T (=VKM B-2499T =CCM 7569T). The type strain of Bauldia litoralis is strain 524-16T (= NCIB 2233T =ATCC 35022T). The type strain of the other species in this genus is Bauldia consociata strain 11T (=VKM B-2498T =CCM 7594T). PMID:20118292

Yee, Benjamin; Oertli, Gary E.; Fuerst, John A.; Staley, James T.

2010-01-01

376

A Robust Protocol to Increase NimbleGen SeqCap EZ Multiplexing Capacity to 96 Samples  

PubMed Central

Contemporary genetic studies frequently involve sequencing of a targeted gene panel, for instance consisting of a set of genes associated with a specific disease. The NimbleGen SeqCap EZ Choice kit is commonly used for the targeted enrichment of sequencing libraries comprising a target size up to 7 Mb. A major drawback of this commercially available method is the exclusive use of single-indexing, meaning that at most 24 samples can be multiplexed in a single reaction. In case of relatively small target sizes, this will lead to excessive amounts of data per sample. We present an extended version of the NimbleGen SeqCap EZ protocol which allows to robustly multiplex up to 96 samples. We achieved this by incorporating Illumina dual-indexing based custom adapters into the original protocol. To further extend the optimization of cost-efficient sequencing of custom target panels, we studied the effect of higher pre-enrichment pooling factors and show that pre-enrichment pooling of up to 12 samples does not affect the quality of the data. To facilitate evaluation of capture efficiency in custom design panels, we also provide a detailed reporting tool. PMID:25875648

van der Werf, Ilse M.; Kooy, R. Frank; Vandeweyer, Geert

2015-01-01

377

Gen-2 RFID compatible, zero down-time, programmable mechanical strain-monitors and mechanical impact detectors  

NASA Astrophysics Data System (ADS)

A key challenge in structural health monitoring (SHM) sensors embedded inside civil structures is that elec- tronics need to operate continuously such that mechanical events of interest can be detected and appropriately analyzed. Continuous operation however requires a continuous source of energy which cannot be guaranteed using conventional energy scavenging techniques. The paper describes a hybrid energy scavenging SHM sensor which experiences zero down-time in monitoring mechanical events of interest. At the core of the proposed sensor is an analog floating-gate storage technology that can be precisely programmed at nano-watt and pico- watt power levels. This facilitates self-powered, non-volatile data logging of the mechanical events of interest by scavenging energy directly from the mechanical events itself. Remote retrieval of the stored data is achieved using a commercial off-the-shelf Gen-2 radio-frequency identification (RFID) reader which periodically reads an electronic product code (EPC) that encapsulates the sensor data. The Gen-2 interface also facilitates in simultaneous remote access to multiple sensors and also facilitates in determining the range and orientation of the sensor. The architecture of the sensor is based on a token-ring topology which enables sensor channels to be dynamically added or deleted through software control.

Chakrabartty, Shantanu; Feng, Tao; Aono, Kenji

2013-04-01

378

Chalicogloea cavernicola gen. nov., sp. nov. (Chroococcales, Cyanobacteria), from low-light aerophytic environments: combined molecular, phenotypic and ecological criteria.  

PubMed

This work characterizes a unicellular cyanobacterium with nearly spherical cells and thin-outlined sheaths that divide irregularly, forming small packets immersed in a diffluent mucilaginous layer. It was isolated growing on calcite speleothems and walls in a show cave in Collbató (Barcelona, Spain). Spectral confocal laser and transmission electron microscopy were used to describe the morphology, fine structure and thylakoid arrangement. The pigments identified were phycoerythrin, phycocyanin, allophycocyanin and chlorophyll a. Three-dimensional reconstructions, generated from natural fluorescence z-stacks, revealed a large surface area of nearly flat, arm-like thylakoidal membranes connected to each other and forming a unified structure in a way that, to our knowledge, has never been described before. Phylogenetic analyses using the 16S rRNA gene sequence showed 95% similarity to strain Chroococcus sp. JJCM (GenBank accession no. AM710384). The diacritical phenotypic features do not correspond to any species currently described, and the genetic traits support the strain being classified as the first member of an independent genus in the order Chroococcales and the family Chroococcaceae. Hence, we propose the name Chalicogloea cavernicola gen. nov., sp. nov. under the provisions of the International Code of Nomenclature for Algae, Fungi and Plants. The type strain of Chalicogloea cavernicola is COLL 3(T) (=CCALA 975(T) =CCAP 1424/1(T)). PMID:23178723

Roldán, M; Ramírez, M; del Campo, J; Hernández-Mariné, M; Komárek, J

2013-06-01

379

Phylotranscriptomics: Saturated Third Codon Positions Radically Influence the Estimation of Trees Based on Next-Gen Data  

PubMed Central

Recent advancements in molecular sequencing techniques have led to a surge in the number of phylogenetic studies that incorporate large amounts of genetic data. We test the assumption that analyzing large number of genes will lead to improvements in tree resolution and branch support using moths in the superfamily Bombycoidea, a group with some interfamilial relationships that have been difficult to resolve. Specifically, we use a next-gen data set that included 19 taxa and 938 genes (?1.2M bp) to examine how codon position and saturation might influence resolution and node support among three key families. Maximum likelihood, parsimony, and species tree analysis using gene tree parsimony, on different nucleotide and amino acid data sets, resulted in largely congruent topologies with high bootstrap support compared with prior studies that included fewer loci. However, for a few shallow nodes, nucleotide and amino acid data provided high support for conflicting relationships. The third codon position was saturated and phylogenetic analysis of this position alone supported a completely different, potentially misleading sister group relationship. We used the program RADICAL to assess the number of genes needed to fix some of these difficult nodes. One such node originally needed a total of 850 genes but only required 250 when synonymous signal was removed. Our study shows that, in order to effectively use next-gen data to correctly resolve difficult phylogenetic relationships, it is necessary to assess the effects of synonymous substitutions and third codon positions. PMID:24148944

Breinholt, Jesse W.; Kawahara, Akito Y.

2013-01-01

380

New Australian stiletto flies: revision of Manestella Metz and description of Medomega gen. n. (Diptera, Therevidae, Agapophytinae)  

PubMed Central

Abstract The previously monotypic genus Manestella Metz, 2003 is revised with a single species, Manestella tristriata (Mann, 1933), redescribed and an additional 14 new species described: Manestella caesia sp. n., Manestella campestris sp. n., Manestella canities sp. n., Manestella cooloola sp. n., Manestella fumosa sp. n., Manestella incompleta sp. n., Manestella nubis sp. n., Manestella obscura sp. n., Manestella ocellaris sp. n., Manestella persona sp. n., Manestella poecilothorax sp. n., Manestella umbrapennis sp. n., Manestella vasta sp. n. and Manestella vespera sp. n. The putative sister genus to Manestella, Medomega gen. n., is described containing six new species: Medomega averyi sp. n., Medomega bailmeup sp. n., Medomega chlamydos sp. n., Medomega danielsi sp. n., Medomega gigasathe sp. n., and Medomega nebrias sp. n. Complete taxonomic descriptions were generated from a character matrix developed in Lucid Builder from which natural language descriptions (NLD) were parsed. Images of all species of Manestella and Medomega gen. n. are included, along with dichotomous keys to species. PMID:23226714

Winterton, Shaun L.; Lambkin, Christine L.

2012-01-01

381

Hemodynamic and Echocardiographic Profiles in African American Compared With White Offspring of Hypertensive Parents: The HyperGEN Study  

PubMed Central

BACKGROUND Alterations in cardiovascular structure and function have been shown to precede the finding of elevated blood pressure. METHODS This study is part of the Hypertension Genetic Epidemiologic Network (HyperGEN) in which genetic and environmental determinants of hypertension were investigated in 5 geographical field centers. All nonhypertensive offspring (n = 1,035) were included from the entire HyperGEN study population that consists of 2,225 hypertensive patients and 1,380 nonhypertensive patients who had adequate echocardiographic left ventricular (LV) mass measurements. Participants were compared by self-declared race (African American and white). RESULTS Nonhypertensive African American offspring were younger (aged 31 years vs. 38 years), more likely to be female, and had a higher body mass index (BMI) and higher systolic blood pressure (SBP) than their white counterparts. After adjusting for age, sex, SBP, pulse pressure (PP), BMI, diabetes status, and family effects, we observed statistically significant and potentially pathophysiological differences (all with P ? 0.001) with greater LV mass/height, relative wall thickness, and posterior wall thickness and with lesser midwall shortening, PP/stroke volume, and (PP/stroke volume)/fat-free body mass. CONCLUSION This study shows that ethnic differences in hemodynamic and echocardiographic profiles exist in a large, population-based cohort of nonhypertensive offspring of hypertensive parents. PMID:24242823

2014-01-01

382

Rhopalobdella japonica n. gen., n. sp. (Hirudinea, Piscicolidae) from Dasyatis akajei (Chondrichthyes: Dasyatididae) in the northwestern Pacific.  

PubMed

A new genus and species of piscicolid leech in the Platybdellinae inhabits the oral cavity of Dasyatis akajei in the northwestern Pacific Ocean near Tanabe, Japan. The genus Rhopalobdella n. gen. is characterized externally by very small oral and caudal suckers and a smooth body that is widest just posterior to the clitellum. Eyespots and ocelli are lacking. The coelom is spacious with large segmental connecting sinuses between dorsal and ventral sinuses. There are 5 pairs of testisacs, an unusually extensive epididymis, and a very large bursa. Conducting tissue is absent. There are 2 pairs of esophageal diverticula and very well developed nephridia. Rhopalobdella japonica n. gen. n. sp. is characterized by a urosome that tapers strongly to the caudal sucker and by a single gonopore; the common oviduct opens into the posterior portion of the bursa. The coelomic and excretory systems resemble Aestabdella, but in other respects the genera are quite different. This is the first marine leech reported from rays in the northwestern Pacific. PMID:10958442

Burreson, E M; Kearn, G C

2000-08-01

383

Mongoliicoccus roseus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake.  

PubMed

Two pink, non-motile, aerobic, alkaliphilic, halotolerant, Gram-negative cocci, designated MIM28(T) and MIM29, were isolated from the surface water of a haloalkaline lake on the Mongolia Plateau. The isolates grew optimally at 30-33 °C, at pH 8-9 and with 3-4 % (w/v) NaCl. The isolates were chemoheterotrophic and could assimilate carbohydrates, organic acids and amino acids. The major respiratory quinone was menaquinone MK-7. The major polar lipids were phosphatidylcholine and phosphatidylethanolamine. The predominant cellular fatty acids were iso-C(15 : 0) (13.8-17.5 %), anteiso-C(15 : 0) (10.5-11.2 %), iso-C(16 : 0) (9.9-13.0 %), C(16 : 0) (4.3-4.6 %), iso-C(17 : 0) (3.8-5.3 %), anteiso-C(17 : 0) (3.7-7.1 %), C(17 : 1)?6c (4.6-6.4 %), iso-C(17 : 0) 3-OH (4.6-5.8 %), summed feature 3 (C(16 : 1)?7c and/or C(16 : 1)?6c; 4.0-6.4 %) and summed feature 9 (iso-C(17 : 1)?9c and/or C(16 : 0) 10-methyl; 10.4-12.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates were most closely related to Litoribacter ruber YIM CH208(T) (93.6 % 16S rRNA gene sequence similarity), the genus Echinicola (90.4-92 %) and other members of the family Cyclobacteriaceae (87.8-90 %). The DNA G+C contents of strains MIM28(T) and MIM29 were 62.8 and 62.2 mol%. On the basis of morphology, physiology, fatty acid composition, phylogeny and 16S rRNA gene sequence analysis, the isolates are assigned to a novel species of a new genus, for which we propose the name Mongoliicoccus roseus gen. nov., sp. nov.; the type strain of the type species is MIM28(T) (= ACCC 05511(T) = KCTC 19808(T)). PMID:22081716

Liu, Ya Peng; Wang, Yong Xia; Li, Yun Xu; Feng, Fu Ying; Liu, Hui Rong; Wang, Jia

2012-09-01

384

Desmozoon lepeophtherii n. gen., n. sp., (Microsporidia: Enterocytozoonidae) infecting the salmon louse Lepeophtheirus salmonis (Copepoda: Caligidae)  

PubMed Central

Background A microsporidian was previously reported to infect the crustacean parasite, Lepeophtheirus salmonis (Krøyer, 1837) (Copepoda, Caligidae), on farmed Atlantic salmon (Salmo salar L.) in Scotland. The microsporidian was shown to be a novel species with a molecular phylogenetic relationship to Nucleospora (Enterocytozoonidae), but the original report did not assign it to a genus or species. Further studies examined the development of the microsporidian in L. salmonis using electron microscopy and re-evaluated the molecular findings using new sequence data available for the group. Here we report a full description for the microsporidian and assign it to a new genus and species. Results The microsporidian infects subcuticular cells that lie on the innermost region of the epidermal tissue layer beneath the cuticle and along the internal haemocoelic divisions. The mature spores are sub-spherical with a single nucleus and an isofilar polar filament with 5-8 turns in a double coil. The entire development is in direct contact with the host cell cytoplasm and is polysporous. During early merogony, a diplokaryotic nuclear arrangement exists which is absent throughout the rest of the developmental cycle. Large merogonial plasmodia form which divide to form single uninucleate sporonts. Sporogonial plasmodia were not observed; instead, binucleate sporonts divide to form two sporoblasts. Prior to final division, there is a precocious development of the polar filament extrusion apparatus which is associated with large electron lucent inclusions (ELIs). Analyses of DNA sequences reveal that the microsporidian is robustly supported in a clade with other members of the Enterocytozoonidae and confirms a close phylogenetic relationship with Nucleospora. Conclusion The ultrastructural findings of the precocious development of the polar filament and the presence of ELIs are consistent with those of the Enterocytozoonidae. However, the confirmed presence of an early diplokaryotic stage and a merogonial plasmodium that divides to yield uninucleate sporonts instead of transforming into a sporogonial syncitium, are features not currently associated with the family. Yet, analyses of DNA sequence data clearly place the microsporidian within the Enterocytozoonidae. Therefore, due to the novelty of the copepod host, the ultrastructural findings and the robust nature of the phylogenetic analyses, a new genus should be created within the Enterocytozoonide; Desmozoon lepeophtherii n. gen. n. sp. is proposed. PMID:19943930

2009-01-01

385

Bombella intestini gen. nov., sp. nov., an acetic acid bacterium isolated from bumble bee crop.  

PubMed

In the frame of a bumble bee gut microbiota study, acetic acid bacteria (AAB) were isolated using a combination of direct isolation methods and enrichment procedures. MALDI-TOF MS profiling of the isolates and a comparison of these profiles with profiles of established AAB species identified most isolates as Asaia astilbis or as 'Commensalibacter intestini', except for two isolates (R-52486 and LMG 28161(T)) that showed an identical profile. A nearly complete 16S rRNA gene sequence of strain LMG 28161(T) was determined and showed the highest pairwise similarity to Saccharibacter floricola S-877(T) (96.5%), which corresponded with genus level divergence in the family Acetobacteraceae. Isolate LMG 28161(T) was subjected to whole-genome shotgun sequencing; a 16S-23S rRNA internal transcribed spacer (ITS) sequence as well as partial sequences of the housekeeping genes dnaK, groEL and rpoB were extracted for phylogenetic analyses. The obtained data confirmed that this isolate is best classified into a new genus in the family Acetobacteraceae. The DNA G+C content of strain LMG 28161(T) was 54.9 mol%. The fatty acid compositions of isolates R-52486 and LMG 28161(T) were similar to those of established AAB species [with C18:1?7c (43.1%) as the major component], but the amounts of fatty acids such as C19:0 cyclo ?8c, C14:0 and C14:0 2-OH enabled to differentiate them. The major ubiquinone was Q-10. Both isolates could also be differentiated from the known genera of AAB by means of biochemical characteristics, such as their inability to oxidize ethanol to acetic acid, negligible acid production from melibiose, and notable acid production from d-fructose, sucrose and d-mannitol. In addition, they produced 2-keto-d-gluconate, but not 5-keto-d-gluconate from d-glucose. Therefore, the name Bombella intestini gen nov., sp. nov. is proposed for this new taxon, with LMG 28161(T) (?=DSM 28636(T)?=R-52487(T)) as the type strain of the type species. PMID:25336723

Li, Leilei; Praet, Jessy; Borremans, Wim; Nunes, Olga C; Manaia, Célia M; Cleenwerck, Ilse; Meeus, Ivan; Smagghe, Guy; De Vuyst, Luc; Vandamme, Peter

2015-01-01

386

Oceanirhabdus sediminicola gen. nov., sp. nov., an anaerobic bacterium isolated from sea sediment.  

PubMed

A novel anaerobic bacterium, designated NH-JN4(T) was isolated from a sediment sample collected in the South China Sea. Cells were Gram-stain-positive, spore-forming, peritrichous and rod-shaped (0.5-1.2×2.2-7 µm). The temperature and pH ranges for growth were 22-42 °C and pH 6.0-8.5. Optimal growth occurred at 34-38 °C and pH 6.5-7.0. The NaCl concentration range for growth was 0.5-6?% (w/v) with an optimum of 2.5?%. Catalase and oxidase were not produced. Substrates which could be utilized were peptone, tryptone, yeast extract, beef extract and glycine. Main fermentation products from PYG medium were formate, acetate, butyrate and ethanol. Strain NH-JN4(T) could utilize sodium sulfite as an electron acceptor. No respiratory quinone was detected. The predominant fatty acids were anteiso-C15?:?0, C16?:?0, iso-C15?:?0, anteiso-C17?:?0 and C16?:?0 DMA. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and glycolipids. The DNA G+C content was 35.8 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain NH-JN4(T) was a member of family Clostridiaceae, and was most closely related to Clostridium limosum ATCC 25620(T), Clostridium proteolyticum DSM 3090(T), Clostridium histolyticum ATCC 19401(T) and Clostridium tepidiprofundi SG 508(T), showing 94.0, 93.0, 92.9 and 92.3?% sequence similarity, respectively. On the basis of phenotypic, genotypic and chemotaxonomic properties, strain NH-JN4(T) represents a novel species of a new genus in the family Clostridiaceae, for which the name Oceanirhabdus sediminicola gen. nov., sp. nov. is proposed. The type strain of the type species is NH-JN4(T) (?=?JCM 18501(T)?=?CCTCC AB 2013103(T)?=?KCTC 15322(T)). PMID:23811141

Pi, Ruo-Xi; Zhang, Wen-Wu; Fang, Ming-Xu; Zhang, Yan-Zhou; Li, Tian-Tian; Wu, Min; Zhu, Xu-Fen

2013-11-01

387

Telmatocola sphagniphila gen. nov., sp. nov., a Novel Dendriform Planctomycete from Northern Wetlands  

PubMed Central

Members of the phylum Planctomycetes are common inhabitants of northern wetlands. We used barcoded pyrosequencing to survey bacterial diversity in an acidic (pH 4.0) Sphagnum peat sampled from the peat bog Obukhovskoye, European North Russia. A total of 21189 bacterial 16S rRNA gene sequences were obtained, of which 1081 reads (5.1%) belonged to the Planctomycetes. Two-thirds of these sequences affiliated with planctomycete groups for which characterized representatives have not yet been available. Here, we describe two organisms from one of these previously uncultivated planctomycete groups. One isolate, strain OB3, was obtained from the peat sample used in our molecular study, while another strain, SP2T (=DSM 23888T?=?VKM B-2710T), was isolated from the peat bog Staroselsky moss. Both isolates are represented by aerobic, budding, pink-pigmented, non-motile, spherical cells that are arranged in unusual, dendriform-like structures during growth on solid media. These bacteria are moderately acidophilic and mesophilic, capable of growth at pH 4.0–7.0 (optimum pH 5.0–5.5) and at 6–30°C (optimum 20–26°C). The preferred growth substrates are various heteropolysaccharides and sugars, the latter being utilized only if provided in low concentrations (?0.025%). In contrast to other described planctomycetes, strains SP2T and OB3 possess weak cellulolytic potential. The major fatty acids are C16:1?5c, C18:1?5c, C16:0, and C18:0. Characteristic lipids are the n-C31 polyunsaturated alkene (9–10 double bonds) and C30:1/C32:1 (?-1) hydroxy fatty acids. The G?+?C content of the DNA is 58.5–59.0?mol%. Strains SP2T and OB3 share identical 16S rRNA gene sequences, which exhibit only 86 and 87% similarity to those of Gemmata obscuriglobus and Zavarzinella formosa. Based on the characteristics reported here, we propose to classify these novel planctomycetes as representatives of a novel genus and species, Telmatocola sphagniphila gen. nov., sp. nov. PMID:22529844

Kulichevskaya, Irina S.; Serkebaeva, Yulia M.; Kim, Yongkyu; Rijpstra, W. Irene C.; Damsté, Jaap S. Sinninghe; Liesack, Werner; Dedysh, Svetlana N.

2012-01-01

388

Kosmotoga olearia gen. nov., sp. nov., a thermophilic, anaerobic heterotroph isolated from an oil production fluid.  

PubMed

A novel thermophilic, heterotrophic bacterium, strain TBF 19.5.1(T), was isolated from oil production fluid at the Troll B oil platform in the North Sea. Cells of strain TBF 19.5.1(T) were non-motile rods with a sheath-like structure, or toga. The strain was Gram-negative and grew at 20-80 degrees C (optimum 65 degrees C), pH 5.5-8.0 (optimum pH 6.8) and NaCl concentrations of 10-60 g l(-1) (optimum 25-30 g l(-1)). For a member of the order Thermotogales, the novel isolate is capable of unprecedented growth at low temperatures, with an optimal doubling time of 175 min (specific growth rate 0.24 h(-1)) and a final optical density of >1.4 when grown on pyruvate at 37 degrees C. Various carbohydrates, proteinaceous compounds and pyruvate served as growth substrates. Thiosulfate, but not elemental sulfur, enhanced growth of the isolate. Sulfate also enhanced growth, but sulfide was not produced. The strain grew in the presence of up to approximately 15 % oxygen, but only if cysteine was included in the medium. Growth of the isolate was inhibited by acetate, lactate and propionate, while butanol and malate prevented growth. The major fermentation products formed on maltose were hydrogen, carbon dioxide and acetic acid, with traces of ethanol and propionic acid. The G+C content of the genomic DNA was 42.5 mol%. Phylogenetic analyses of the 16S and 23S rRNA gene sequences as well as 29 protein-coding ORFs placed the strain within the bacterial order Thermotogales. Based on the phylogenetic analyses and the possession of a variety of physiological characteristics not previously found in any species of this order, it is proposed that the strain represents a novel species of a new genus within the family Thermotogaceae, order Thermotogales. The name Kosmotoga olearia gen. nov., sp. nov. is proposed. The type strain of Kosmotoga olearia is TBF 19.5.1(T) (=DSM 21960(T) =ATCC BAA-1733(T)). PMID:19643902

Dipippo, Jonathan L; Nesbø, Camilla L; Dahle, Håkon; Doolittle, W Ford; Birkland, Nils-Kåre; Noll, Kenneth M

2009-12-01

389

INSTITUTE OF PHYSICS PUBLISHING JOURNAL OF PHYSICS A: MATHEMATICAL AND GENERAL J. Phys. A: Math. Gen. 36 (2003) 90539066 PII: S0305-4470(03)59357-X  

E-print Network

force failed to provide a good approximation for helium. But in 1921, Irving Langmuir (who would receive. Gen. 36 (2003) 9053­9066 PII: S0305-4470(03)59357-X On the dynamics of the Langmuir problem Florin set of the classical Coulomb atom, which generalizes the one Langmuir proposed for helium in 1921

Diacu, Florin

2003-01-01

390

Poster Session--Predicting Patterns of Alien Plant Invasion--Underwood, Klinger, Moore USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 361  

E-print Network

Poster Session--Predicting Patterns of Alien Plant Invasion--Underwood, Klinger, Moore USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 361 Predicting Patterns of Alien Plant Invasions in Areas of alien plant species. This is particularly problematic in areas which have experienced disturbances

Standiford, Richard B.

391

Poster Session--Fuel Consumption During Prescribed Fires in Big Sage--Wright, Ottmar USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 363  

E-print Network

Poster Session--Fuel Consumption During Prescribed Fires in Big Sage--Wright, Ottmar USDA Forest conditions can be mitigated in some cases, by adjustments to firing techniques. Methods Relationships gleaned Service Gen. Tech. Rep. PSW-GTR-189. 2008. 363 Fuel Consumption During Prescribed Fires in Big Sage

Standiford, Richard B.

392

Gen. Tech. Rep. PSW-101 Berkeley, CA. Pacific Southwest Forest and Range Experiment Station, Forest Service, U.S. Department of Agriculture; 1987.  

E-print Network

governments and fire services have underutilized existing authorities in describing and mitigating fireGen. Tech. Rep. PSW-101 Berkeley, CA. Pacific Southwest Forest and Range Experiment Station, Forest-structures-wildland that is different from the traditional "urban-wildland interface." The fire and structural environment for seven

Standiford, Richard B.

393

USDA Forest Service Gen. Tech. Rep. RMRS-GTR-42-vol. 6. 2008 33 The alteration of fire regimes is one of the most  

E-print Network

USDA Forest Service Gen. Tech. Rep. RMRS-GTR-42-vol. 6. 2008 33 The alteration of fire regimes). Directinterferenceofinvaderswithnativeplantscan be mitigated by removing or controlling the invading species. In contrast, when invaders cause in which invasions by nonnative plant species can change fuel conditions and fire regimes, and discuss what

394

CSU, FULLERTON CREDIT FOR EXTERNAL EXAMS (AP, IB, CLEP) ADVANCED PLACEMENT EXAMS (AP) SCORE UNITS COURSE CREDIT GEN EDUC BEGIN DATE END DATE  

E-print Network

CSU, FULLERTON CREDIT FOR EXTERNAL EXAMS (AP, IB, CLEP) ADVANCED PLACEMENT EXAMS (AP) SCORE UNITS ENGL 101 GE I.B 3 ENGL 105 GE III.B.2 Updated 11/30/10 #12;CSU, FULLERTON CREDIT FOR EXTERNAL EXAMS (AP, IB, CLEP) ADVANCED PLACEMENT EXAMS (AP) SCORE UNITS COURSE CREDIT GEN EDUC BEGIN DATE END DATE AP

de Lijser, Peter

395

Session B--Small-Diameter Timber Alchemy--Fried, Barbour, Fight, Christensen, Pinjuv USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 29  

E-print Network

Session B--Small-Diameter Timber Alchemy--Fried, Barbour, Fight, Christensen, Pinjuv USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 29 Small-Diameter Timber Alchemy: Can Utilization Pay the Way- mail: Jeremy.Fried@fs.fed.us. #12;Session B--Small-Diameter Timber Alchemy--Fried, Barbour, Fight

Standiford, Richard B.

396

Print this Meridian Article Gen Y Goes to College: Perceptions of Former Middle School Students and the Graduate Students They Mentored  

Microsoft Academic Search

This paper describes the perceptions and attitudes of former middle school (now high school) students and the graduate students they assisted during university multimedia classes offered over a five-year period. Gen Y focuses on today's youth, enabling them to become contributing partners - and often leaders - in bringing technology into classrooms and communities. This project encouraged authentic participation of

Alice Christie; Valerie Naish; Jayme Kelter; Joey Wycoff; Cory Pearman

397

J. Phys. A: Math. Gen. 30 (1997) 4199--4214. Printed in the UK PII: S03054470(97)814825 Dynamics of lattice vibrations for onedimensional  

E-print Network

. The incommensurability of the composites comes neither from the modulation of a basic three­dimensional (3D) crystallineJ. Phys. A: Math. Gen. 30 (1997) 4199--4214. Printed in the UK PII: S0305­4470(97)81482­5 Dynamics quasicrystals. 1. Introduction The acceptance of the name `composite' in materials science is large, applying

Radulescu, Ovidiu

398

Genome-Wide Detection of Allele Specific Copy Number Variation Associated with Insulin Resistance in African Americans from the HyperGEN Study  

Microsoft Academic Search

African Americans have been understudied in genome wide association studies of diabetes and related traits. In the current study, we examined the joint association of single nucleotide polymorphisms (SNPs) and copy number variants (CNVs) with fasting insulin and an index of insulin resistance (HOMA-IR) in the HyperGEN study, a family based study with proband ascertainment for hypertension. This analysis is

Marguerite R. Irvin; Nathan E. Wineinger; Treva K. Rice; Nicholas M. Pajewski; Edmond K. Kabagambe; Charles C. Gu; Jim Pankow; Kari E. North; Jemma B. Wilk; Barry I. Freedman; Nora Franceschini; Uli Broeckel; Hemant K. Tiwari; Donna K. Arnett; Giorgio Sesti

2011-01-01

399

J. Phys. A: Math. Gen. 17 (1984) L843-L847. Printed in Great Britain L E m R TO THE EDITOR  

E-print Network

J. Phys. A: Math. Gen. 17 (1984) L843-L847. Printed in Great Britain L E m R TO THE EDITOR Received 31 July 1984 Abstract. Numerical simulationof the two-dimensionalbond percolationat 1980, Pike and Stanley 1981, Hong and Stanley 1983a,b, Hermann et a1 1984), as opposed to the shapes

Kantor, Yacov

400

J. Phys. A: Math. Gen. 17 (1984) L933-L938. Printed in Great Britain LE'ITER TO THE EDITOR  

E-print Network

J. Phys. A: Math. Gen. 17 (1984) L933-L938. Printed in Great Britain LE'ITER TO THE EDITOR for Polymer Studies4 and Department of Physics, Boston University, Boston MA 02215, USA Received 2 August 1984 (Cardy 1984a, b). In this letter we test some of these predictions for self-avoiding random walks (SAWS

Redner, Sidney

401

J. Phys. A: Math. Gen. 17 (1984) L665-L670. Printed in Great Britain LE'ITER TO THE EDITOR  

E-print Network

J. Phys. A: Math. Gen. 17 (1984) L665-L670. Printed in Great Britain LE'ITER TO THE EDITOR Received 22 May 1984 Abstract. We present a scaling approach to investigate the kinetics of the diffusion 1984,Meakin and Stanley 1984). On the other hand, the rate equation predicts a decay of l/(kt) or exp

Redner, Sidney

402

J. Phys. A: Math. Gen. 17 (1984) 3223-3236. Printed in Great Britain Renormalisation group study of a disordered model  

E-print Network

J. Phys. A: Math. Gen. 17 (1984) 3223-3236. Printed in Great Britain Renormalisation group study, France Received 1 1 April 1984 Abstract. We study the Potts model on a diamond hierarchical latticeKay and Berker 1984, Kaufman and Andelman 1984)in particular to distinguish between the features which

Derrida, Bernard

403

Appl Nbr Full TiFirst Name Middle Name Last Name Sex Category PH Category 00036777 F ADITI GUPTA F GEN N  

E-print Network

Appl Nbr Full TiFirst Name Middle Name Last Name Sex Category PH Category 00036777 F ADITI GUPTA F 00036674 F SRISHTI VIVEK SHARMA F GEN N Appl Nbr Full TiFirst Name Middle Name Last Name Sex Category PH) Appl Nbr Full TiFirst Name Middle Name Last Name Sex Category PH Category 00032299 F SANDEEP KUMAR

Ribeiro, Vinay

404

Session I--External Influences in Presuppression Planning--Wiitala, Wilson USDA Forest Service Gen. Tech. Rep. PSW-GTR-189 2008 247  

E-print Network

Session I--External Influences in Presuppression Planning--Wiitala, Wilson USDA Forest Service Gen. Tech. Rep. PSW-GTR-189 2008 247 The Importance of Considering External Influences During Presuppression of nationally shared resources impacted by wildfire activity at the regional and national scale. These external

Standiford, Richard B.

405

Session H--NEPA Disclosure of Air Quality Impacts--Ahuja, Perrot USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008.  

E-print Network

Session H--NEPA Disclosure of Air Quality Impacts--Ahuja, Perrot USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. . 193 National Environmental Policy Act Disclosure of Air Quality Impacts air quality and has the potential to impact human health and quality of life. Public concern about

Standiford, Richard B.

406

Session K--Assessing the Effectiveness of Landscape Fuel Treatments--Stratton USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 309  

E-print Network

Session K--Assessing the Effectiveness of Landscape Fuel Treatments--Stratton USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 309 Assessing the Effectiveness of Landscape Fuel Treatments for assessing the effectiveness of landscape fuel treatments on fire growth and behavior. Treatment areas were

Standiford, Richard B.

407

Session L--Effects of Fire on Serpentine Vegetation--Safford, Harrison USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 321  

E-print Network

Session L--Effects of Fire on Serpentine Vegetation--Safford, Harrison USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 321 The Effects of Fire on Serpentine Vegetation and Implications fire effects on adjacent serpentine and non-serpentine soils in two vegetation types, grassland

Standiford, Richard B.

408

329 J. Gen. Physiol. The Rockefeller University Press 0022-1295/2001/04/329/15 $5.00 Volume 117 April 2001 329343  

E-print Network

329 J. Gen. Physiol. © The Rockefeller University Press · 0022-1295/2001/04/329/15 $5.00 Volume 117 abstract Intracellular organelles have characteristic pH ranges that are set and maintained by a balance be in pH regulation; however, recent experiments show that cellular compartments are not in thermodynamic

Oster, George

409

Poster Session--Effectiveness of Regreen Seeding for Erosion Control--Beyers USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008.330  

E-print Network

Poster Session--Effectiveness of Regreen Seeding for Erosion Control--Beyers USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008.330 Growth of Regreen, Seeded for Erosion Control, in the Manter controlled on September 6 after burning 32,074 ha (79,244 ac) of National Forest System, Bureau of Land

Standiford, Richard B.

410

Poster Session--Prescribed Fire Effects on California's Oaks--Paysen USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008.352  

E-print Network

. Figure 1--Post fire erosion problem. Figure 2--U.S. Forest Service prescribed burning program. 1 A poster of studies are in place (figs: 4-8). Figure 4--Thin and understory burn to produce a shaded fuel break Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008.354 Figure 5--Prescribed burn of invasive grass in oak

Standiford, Richard B.

411

Evaluation of the Gen-Probe Aptima HIV-1 RNA Qualitative Assay as an Alternative to Western Blot Analysis for Confirmation of HIV Infection?  

PubMed Central

The Gen-Probe Aptima HIV-1 RNA qualitative assay was evaluated as an alternative to Western blot analysis for the confirmation of HIV infection using serum samples that were repeatedly reactive for HIV antibodies. The Aptima HIV assay readily discriminated between HIV-1-infected and -uninfected individuals and effectively reduced the number of indeterminate results relative to Western blot analysis. PMID:21346052

Pierce, Virginia M.; Neide, Brandy; Hodinka, Richard L.

2011-01-01

412

Session A--Equipping Tomorrow's Fire Managers--Dicus USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 1  

E-print Network

Session A--Equipping Tomorrow's Fire Managers--Dicus USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 1 Equipping Tomorrow's Fire Managers1 Christopher A. Dicus2 Abstract Fire managers's managers must be equipped with the skills necessary to understand and liaise with a burgeoning group

Standiford, Richard B.

413

Session I--Communication of GIS Data in Fire Management--Boykin and others USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 209  

E-print Network

. For the inclusion of these tools, fire management and research personnel must be effective in communication of personnel can be involved within fire management including land managers, landowners, natural resourceSession I-- Communication of GIS Data in Fire Management--Boykin and others USDA Forest Service Gen

Standiford, Richard B.

414

Film and Language Learning in Victorian Schools: A Study of the 2009 Next Gen Program of the "Melbourne International Film Festival"  

ERIC Educational Resources Information Center

This article investigates the role of film in language education based on a study of the 2009 "Melbourne International Film Festival" (MIFF). It is structured around a literature review and results from surveys of students and teachers who participated in the young people's section of the program, Next Gen. The article argues that film can provide…

Lo Bianco, Hana

2010-01-01

415

DIETS CONTAINING SOY PROTEIN ISOLATE (SPI) OR GENISTEIN (GEN) FED TO FEMALE RATS DURING PREGNANCY DO NOT SUPPRESS AZOXYMETHANE (AOM)-INDUCED INTESTINAL CANCERS IN THE MALE PROGENY  

Technology Transfer Automated Retrieval System (TEKTRAN)

Previous studies showed that lifetime dietary SPI inhibits AOM-induced colon tumorigenesis when compared to casein (CAS)-containing control diet. The aim of this study was to investigate if cancer protection by dietary SPI or the soy isoflavone GEN is afforded in utero to progeny via dietary exposu...

416

Session K--Fuel Loadings in Forests, Woodlands, and Savannas--Ffolliott, Gottfried, DeBano USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 271  

E-print Network

Session K--Fuel Loadings in Forests, Woodlands, and Savannas--Ffolliott, Gottfried, DeBano USDA Forest Service Gen. Tech. Rep. PSW-GTR-189. 2008. 271 Fuel Loadings in Forests, Woodlands, and Savannas to increase the loadings of downed woody fuels. Ecologists and land managers are reintroducing fire

Standiford, Richard B.

417

29USDA Forest Service Gen. Tech. Rep. PSW-GTR-155. 1996. Statistical Considerations for Plot Design, Sampling Procedures, Analysis... Arbaugh and Bednar  

E-print Network

Statistician,Pacific NorthwestResearchStation,P.O. Box 3890, Portland, OR, 97208 Statistical Considerations for Plot Design29USDA Forest Service Gen. Tech. Rep. PSW-GTR-155. 1996. Statistical Considerations for Plot Design present recommendations in this chapter to develop plot design and sampling strategies that follow

Standiford, Richard B.

418

Toxopsis calypsus gen. nov., sp. nov. (Cyanobacteria, Nostocales) from cave 'Francthi', Peloponnese, Greece: a morphological and molecular evaluation.  

PubMed

Representatives of a new cyanobacterial genus, Toxopsis Lamprinou & Pantazidou gen. nov., were found in fresh material from Cave 'Francthi' (Peloponnese, Greece) and isolated in cultures. Ecological data relating to the environmental parameters of the sampling sites are provided, such as the photosynthetically active radiation (PAR), temperature and relative humidity. Morphological characteristics and the life cycle of the type species Toxopsis calypsus Lamprinou & Pantazidou sp. nov. were studied using light microscopy and scanning and transmission microscopy. Molecular analysis based on the 16S rRNA gene sequence was also conducted. Toxopsis calypsus sp. nov. is a false-branched nostocalean cyanobacterium with both isopolar and heteropolar filaments bearing mono-pored and bi-pored heterocysts, and also hormogonia and akinetes. Isopolar filaments adhere by the centre to the substrate and are found mainly in fresh material and in young cultures; heteropolar filaments bearing a basic mono-pore heterocyst are dominant in aged (more than one-year-old) cultures. According to the revised taxonomic classification system of Komárek & Anagnostidis (1989) [Komárek, J. & Anagnostidis, K. (1989). Algol Stud, 56, 247-345] based mainly on morphological data, the new genus described here shares morphological characters with both nostocalean families Scytonemataceae and Microchaetaceae, showing similarities in particular to Scytonematopsis contorta [Vaccarino, M. A. & Johansen, J. R. (2011). Fottea 11, 149-161], Microchaetaceae. Molecular data from the 16S rRNA sequence determined in this paper showed that Toxopsis calypsus sp. nov. is more related to the family Microchaetaceae, and the five phylotypes analysed by PCR showed that the closest nostocalean relatives are Tolypothrix distorta SAG 93.79 (GenBank accession no. GQ287651) and Coleodesmium sp. ANT.L52B.5 (AY493596) with 95-96% and 96% similarity, respectively. In contrast, the five phylotypes showed a distant similarity to Scytonematopsis contorta (<91%). The phenotypic and genetic traits strongly supported the classification of the five phylotypes as a new taxon for which the name Toxopsis calypsus Lamprinou & Pantazidou gen. nov., sp. nov. is proposed. PMID:22247210

Lamprinou, V; Skaraki, K; Kotoulas, G; Economou-Amilli, A; Pantazidou, A

2012-12-01

419

Proposal to unify Clostridium orbiscindens Winter et al. 1991 and Eubacterium plautii (Séguin 1928) Hofstad and Aasjord 1982, with description of Flavonifractor plautii gen. nov., comb. nov., and reassignment of Bacteroides capillosus to Pseudoflavonifractor capillosus gen. nov., comb. nov.  

PubMed

We isolated several strains from various clinical samples (five samples of blood, four of intra-abdominal pus and one of infected soft tissue) that were anaerobic, motile or non-motile and Gram-positive rods. Some of the strains formed spores. Phylogenetic analysis of the 16S rRNA gene sequence showed that these organisms could be placed within clostridial cluster IV as defined by Collins et al. [(1994). Int J Syst Bacteriol 44, 812-826] and shared more than 99 % sequence similarity with Clostridium orbiscindens DSM 6740(T) and Eubacterium plautii DSM 4000(T). Together, they formed a distinct cluster, with Bacteroides capillosus ATCC 29799(T) branching off from this line of descent with sequence similarities of 97.1-97.4 %. The next nearest neighbours of these organisms were Clostridium viride, Oscillibacter valericigenes, Papillibacter cinnamivorans and Sporobacter termitidis, with sequence similarities to the respective type strains of 93.1-93.4, 91.2-91.4, 89.8-90 and 88.7-89.3 %. On the basis of biochemical properties, phylogenetic position, DNA G+C content and DNA-DNA hybridization, it is proposed to unify Clostridium orbiscindens and Eubacterium plautii in a new genus as Flavonifractor plautii gen. nov., comb. nov., with the type strain Prévot S1(T) (=ATCC 29863(T) =VPI 0310(T) =DSM 4000(T)), and to reassign Bacteroides capillosus to Pseudoflavonifractor capillosus gen. nov., comb. nov., with the type strain CCUG 15402A(T) (=ATCC 29799(T) =VPI R2-29-1(T)). PMID:19654357

Carlier, Jean-Philippe; Bedora-Faure, Marie; K'ouas, Guylène; Alauzet, Corentine; Mory, Francine

2010-03-01

420

Mooreia alkaloidigena gen. nov., sp. nov. and Catalinimonas alkaloidigena gen. nov., sp. nov., alkaloid-producing marine bacteria in the proposed families Mooreiaceae fam. nov. and Catalimonadaceae fam. nov. in the phylum Bacteroidetes  

PubMed Central

Bacterial strains CNX-216T and CNU-914T were isolated from marine sediment samples collected from Palmyra Atoll and off Catalina Island, respectively. Both strains were Gram-negative and aerobic and produce deep-orange to pink colonies and alkaloid secondary metabolites. Cells of strain CNX-216T were short, non-motile rods, whereas cells of strain CNU-914T were short, curved rods with gliding motility. The DNA G+C contents of CNX-216T and CNU-914T were respectively 57.7 and 44.4 mol%. Strains CNX-216T and CNU-914T contained MK-7 as the predominant menaquinone and iso-C15?:?0 and C16?:?1?5c as the major fatty acids. Phylogenetic analyses revealed that both strains belong to the order Cytophagales in the phylum Bacteroidetes. Strain CNX-216T exhibited low 16S rRNA gene sequence identity (87.1?%) to the nearest type strain, Cesiribacter roseus 311T, and formed a well-supported lineage that is outside all currently described families in the order Cytophagales. Strain CNU-914T shared 97.6?% 16S rRNA gene sequence identity with ‘Porifericola rhodea’ N5EA6-3A2B and, together with ‘Tunicatimonas pelagia’ N5DB8-4 and four uncharacterized marine bacteria isolated as part of this study, formed a lineage that is clearly distinguished from other families in the order Cytophagales. Based on our polyphasic taxonomic characterization, we propose that strains CNX-216T and CNU-914T represent novel genera and species, for which we propose the names Mooreia alkaloidigena gen. nov., sp. nov. (type strain CNX-216T ?=?DSM 25187T ?=?KCCM 90102T) and Catalinimonas alkaloidigena gen. nov., sp. nov. (type strain CNU-914T ?=?DSM 25186T ?=?KCCM 90101T) within the new families Mooreiaceae fam. nov. and Catalimonadaceae fam. nov. PMID:22753528

Choi, Eun Ju; Beatty, Deanna S.; Paul, Lauren A.; Fenical, William

2013-01-01

421

Vulgatibacter incomptus gen. nov., sp. nov. and Labilithrix luteola gen. nov., sp. nov., two myxobacteria isolated from soil in Yakushima Island, and the description of Vulgatibacteraceae fam. nov., Labilitrichaceae fam. nov. and Anaeromyxobacteraceae fam. nov.  

PubMed

Two myxobacterial strains (designated B00001(T) and B00002(T)) were isolated from forest soil samples collected from Yakushima Island, Kagoshima, Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains B00001(T) and B00002(T) respectively formed independent branches within the suborders Cystobacterineae and Sorangiineae and were most closely related to Cystobacter armeniaca DSM 14710(T) (90.4% similarity) and Byssovorax cruenta DSM 14553(T) (91.3%). Neither strain showed typical features of myxobacteria such as bacteriolytic action or fruiting body formation, but both had high DNA G+C contents (66.3-68.3 mol%). Swarming motility was observed in strain B00002(T) only. Cells of both strains were vegetative, chemoheterotrophic, mesophilic, strictly aerobic, Gram-negative, motile rods, and both strains exhibited esterase lipase (C8), leucine arylamidase, naphthol-AS-BI-phosphohydrolase and ?-galactosidase activities. Strain B00001(T) contained MK-7 as the predominant respiratory quinone and the major fatty acid was iso-C15:0. In contrast, strain B00002(T) contained MK-8 as the major cellular quinone and the major fatty acids were C16?:?1?5c and iso-C17?:?0. Based on the phenotypic and genotypic data presented, strains B00001(T) and B00002(T) represent novel genera and species, for which we propose the names Vulgatibacter incomptus gen. nov., sp. nov. and Labilithrix luteola gen. nov., sp. nov., respectively. The type strains of Vulgatibacter incomptus and Labilithrix luteola are B00001(T) (?=?NBRC 109945(T) = DSM 27710(T)) and B00002(T) ( = NBRC 109946(T) = DSM 27648(T)), respectively. The new genera are assigned to the new families Vulgatibacteraceae fam. nov. and Labilitrichaceae fam. nov., respectively. In addition, Anaeromyxobacteraceae fam. nov., is proposed to accommodate the genus Anaeromyxobacter, which is related to the genus Vulgatibacter. PMID:25048208

Yamamoto, Eisaku; Muramatsu, Hideyuki; Nagai, Koji

2014-10-01

422

Plastorhodobacter daqingensis gen. nov., sp. nov.: A Non-phototrophic Bacterium Isolated from Daqing Oilfield.  

PubMed

Two aerobic Gram staining negative, non-motile, and rod-shaped strains, DQW12E81-30(T) and DQW12E6-37-1, were isolated from an oil production mixture from Daqing Oilfield, northeastern China. Phylogenetic analysis based on the nearly complete 16S rRNA gene sequences revealed that strains DQW12E81-30(T) and DQW12E6-37-1 were members of family Rhodobacteraceae, which showed 95.6-95.9 % of 16S rRNA gene sequence similarities with Pararhodobacter aggregans DSM 18938(T), Rhodobacter veldkampii CGMCC 1.5006(T), and Roseinatronobacter thiooxidans DSM 13087(T), and lower similarities (<95.1 %) with all the left type species. Growth of strains DQW12E81-30(T) and DQW12E6-37-1 occurred at pH 7-8, 15-45 °C, and 0-4 % (w/v) of NaCl. The strains could grow both in dark and in light, but neither photosynthetic pigments nor photosynthetic reaction center gene pufM were detected in the strains. These photosynthesis-related features of the two isolates were different from those of Rhodobacter and Roseinatronobacter bacteria, but similar with those of Pararhodobacter. The genomic DNA G+C contents of strains DQW12E81-30(T) and DQW12E6-37-1 were 66.9 and 63.7 mol%, respectively. The predominant ubiquinone was Q-10 for both the strains. The major polar lipids of strain DQW12E81-30(T) were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, unidentified aminolipid, unidentified glycolipid, and unidentified phospholipid. The two strains had C18:1 ?7c, C18:0, and C18:1 ?7c 11-methyl as the major fatty acids. In addition, the strains DQW12E81-30(T) and DQW12E6-37-1 had C16:1 ?7c/C16:1 ?6c, C12:0, C14:0, C14:0 3-OH/C16:1 iso I, C10:0 3-OH, which were remarkably different from those of Pararhodobacter and Roseinatronobacter. The results of phenotypic, genotypic, and chemotaxonomic characteristics analyses indicated that strains DQW12E81-30(T) and DQW12E6-37-1 were readily different from their most phylogenetically closely related genera. Plastorhodobacter daqingensis gen. nov, sp. nov. is proposed for strains DQW12E81-30(T) and DQW12E6-37-1. The type strain is DQW12E81-30(T) (=LMG 27732(T)=CGMCC 1.12750(T)). PMID:25572494

Xie, Bai-Sheng; Lv, Xiang-Lin; Cai, Man; Tang, Yue-Qin; Wang, Ya-Nan; Cui, Heng-Lin; Liu, Xue-Ying; Tan, Yan; Wu, Xiao-Lei

2015-05-01

423

Novel chemolithotrophic, thermophilic, anaerobic bacteria Thermolithobacter ferrireducens gen. nov., sp. nov. and Thermolithobacter carboxydivorans sp. nov.  

PubMed

Three thermophilic strains of chemolithoautotrophic Fe(III)-reducers were isolated from mixed sediment and water samples (JW/KA-1 and JW/KA-2(T): Calcite Spring, Yellowstone N.P., WY, USA; JW/JH-Fiji-2: Savusavu, Vanu Levu, Fiji). All were Gram stain positive rods (approximately 0.5 x 1.8 microm). Cells occurred singly or in V-shaped pairs, and they formed long chains in complex media. All utilized H(2) to reduce amorphous iron (III) oxide/hydroxide to magnetite at temperatures from 50 to 75 degrees C (opt. approximately 73 degrees C). Growth occurred within the pH(60C) range of 6.5-8.5 (opt. pH(60C) 7.1-7.3). Magnetite production by resting cells occurred at pH(60C) 5.5-10.3 (opt. 7.3). The iron (III) reduction rate was 1.3 mumol Fe(II) produced x h(-1) x ml(-1) in a culture with 3 x 10(7) cells, one of the highest rates reported. In the presence or absence of H(2), JW/KA-2(T) did not utilize CO. The G + C content of the genomic DNA of the type strain is 52.7 +/- 0.3 mol%. Strains JW/KA-1 and JW/KA-2(T) each contain two different 16S rRNA gene sequences. The 16S rRNA gene sequences from JW/KA-1, JW/KA-2(T), or JW/JH-Fiji-2 possessed >99% similarity to each other but also 99% similarity to the 16S rRNA gene sequence from the anaerobic, thermophilic, hydrogenogenic CO-oxidizing bacterium 'Carboxydothermus restrictus' R1. DNA-DNA hybridization between strain JW/KA-2(T) and strain R1(T) yielded 35% similarity. Physiological characteristics and the 16S rRNA gene sequence analysis indicated that the strains represent two novel species and are placed into the novel genus Thermolithobacter within the phylum 'Firmicutes'. In addition, the levels of 16S rRNA gene sequence similarity between the lineage containing the Thermolithobacter and well-established members of the three existing classes of the 'Firmicutes' is less than 85%. Therefore, Thermolithobacter is proposed to constitute the first genus within a novel class of the 'Firmicutes', Thermolithobacteria. The Fe(III)-reducing Thermolithobacter ferrireducens gen. nov., sp. nov. is designated as the type species with strain JW/KA-2(T) (ATCC 700985(T), DSM 13639(T)) as its type strain. Strain R1(T) is the type strain for the hydrogenogenic, CO-oxidizing Thermolithobacter carboxydivorans sp. nov. (DSM 7242(T), VKM 2359(T)). PMID:17021657

Sokolova, T; Hanel, J; Onyenwoke, R U; Reysenbach, A-L; Banta, A; Geyer, R; González, J M; Whitman, W B; Wiegel, J

2007-01-01

424

Progress reports for Gen IV sodium fast reactor activities FY 2007.  

SciTech Connect

An important goal of the US DOE Sodium Fast Reactor (SFR) program is to develop the technology necessary to increase safety margins in future fast reactor systems. Although no decision has been made yet about who will build the next demonstration fast reactor, it seems likely that the construction team will include a combination of international companies, and the safety design philosophy for the reactor will reflect a consensus of the participating countries. A significant amount of experience in the design and safety analysis of Sodium Fast Reactors (SFR) using oxide fuel has been developed in both Japan and France during last few decades. In the US, the traditional approach to reactor safety is based on the principle of defense-in-depth, which is usually expressed in physical terms as multiple barriers to release of radioactive material (e.g. cladding, reactor vessel, containment building), but it is understood that the 'barriers' may consist of active systems or even procedures. As implemented in a reactor design, defense-in-depth is classed in levels of safety. Level 1 includes measures to specify and build a reliable design with significant safety margins that will perform according to the intentions of the designers. Level 2 consists of additional design measures, usually active systems, to protect against unlikely accidental events that may occur during the life of the plant. Level 3 design measures are intended to protect the public in the event of an extremely unlikely accident not foreseen to occur during the plant's life. All of the design measures that make up the first three levels of safety are within the design basis of the plant. Beyond Level 3, and beyond the normal design basis, there are accidents that are not expected to occur in a whole generation of plants, and it is in this class that severe accidents, i.e. accidents involving core melting, are included. Beyond design basis measures to address severe accidents are usually identified as being for prevention of progression into severe accident conditions (prevention of core melting) or for mitigation of severe accident consequences (mitigation of the impact of core melting to protect public health and safety). Because design measures for severe accident prevention and mitigation are beyond the normal design basis, established regulatory guidelines and codes do not provide explicit identification of the design performance requirements for severe accident accommodation. The treatment of severe accidents is one of the key issues of R&D plans for the Gen IV systems in general, and for the Sodium Fast Reactor (SFR) in particular. Despite the lack of an unambiguous definition of safety approach applicable for severe accidents, there is an emerging consensus on the need for their consideration for the design. The US SFR program and Argonne National Laboratory (ANL) in particular have actively studied the potential scenarios and consequences of Hypothetical Core Disruptive Accidents (HCDA) for SFRs with oxide fuel during the Fast Flux Test Facility (FFTF) and Clinch River Breeder Reactor Plant (CRBRP) programs in the 70s and 80s. Later, the focus of the US SFR safety R&D activities shifted to the prevention of all HCDAs through passive safety features of the SFRs with metal fuel in the Integral Fast Reactor (IFR) program, and the study of severe accident consequences was de-emphasized. The goal of this paper is to provide an overview of the current SFR safety approach and the role of severe accidents in Japan and France, in preparation for an expected and more active collaboration in this area between the US, Japan, and France.

Cahalan, J. E.; Tentner, A. M.; Nuclear Engineering Division

2007-10-04

425

Predominant Catalase-negative Soil Bacteria. III. Agromyces, gen. n., Microorganisms Intermediary to Actinomyces and Nocardia1  

PubMed Central

The occurrence of filamentous, branching, catalase-negative bacteria as a numerically predominant microflora of various soils was demonstrated by using a dilution frequency isolation procedure. The major characteristics of these organisms were those of the order Actinomycetales. However, they could not be placed in any of the present genera of this order and, therefore, a new genus, Agromyces, was proposed for these organisms. This genus includes catalase-negative, nutritionally-fastidious microorganisms whose cells produce a true branching mycelium that fragments into coccoid and diphtheroid forms. Also, they have an oxidative metabolism, are microaerophilic to aerobic, and contain neither diaminopimelic acid nor lysine as major constituents of the cell wall glycopeptide. The type species would be Agromyces ramosus, gen. n., sp. n. The possible importance of these organisms in clarifying certain phylogenetic relationships of the Actinomycetales is discussed. Images PMID:16349860

Gledhill, William E.; Casida, L. E.

1969-01-01

426

Characterization of some actinomyces-like isolates from human clinical sources: description of Varibaculum cambriensis gen nov, sp nov.  

PubMed

Fifteen strains of an anaerobic, catalase-negative, gram-positive diphtheroid-shaped bacterium recovered from human sources were characterized by phenotypic and molecular chemical and molecular genetic methods. The unidentified bacterium showed some resemblance to Actinomyces species and related taxa, but biochemical testing, polyacrylamide gel electrophoresis analysis of whole-cell proteins, and amplified 16S ribosomal DNA restriction analysis indicated the strains were distinct from all currently named Actinomyces species and related taxa. Comparative 16S rRNA gene sequencing studies showed that the bacterium represents a hitherto-unknown phylogenetic line that is related to but distinct from Actinomyces, Actinobaculum, Arcanobacterium, and Mobiluncus: We propose, on the basis of phenotypic and phylogenetic evidence, that the unknown bacterium from human clinical specimens should be classified as a new genus and species, Varibaculum cambriensis gen. nov., sp. nov. The type strain of Varibaculum cambriensis sp. nov. is CCUG 44998(T) = CIP 107344(T). PMID:12574260

Hall, Val; Collins, Matthew D; Lawson, Paul A; Hutson, Roger A; Falsen, Enevold; Inganas, Elisabeth; Duerden, Brian

2003-02-01

427

Leptohelia flexibilis gen. nov. et sp. nov., a remarkable deep-sea stylasterid (Cnidaria: Hydrozoa: Stylasteridae) from the southwest Pacific.  

PubMed

Leptohelia flexibilis gen. nov. et sp. nov., the first stylasterid with a combined calcified and non-calcified skeleton, is described from seamounts and the slope off the islands of New Caledonia, in the southwestern Pacific. The new species is distinguished from all other species of the family Stylasteridae by having a non-calcified organic axis, internal to the basal portion of the calcified corallum. The internal axis is flexible and enclosed by a series of up to 10 calcified annuli, allowing passive lateral bending of the colony. Molecular phylogenetic analyses confirm that Leptohelia flexibilis is a stylasterid coral and reveal that the species is closely related to Leptohelia microstylus comb. nov., a southwestern Pacific stylasterid that lacks an internal axis. PMID:25543758

Lindner, Alberto; Cairns, Stephen D; Zibrowius, Helmut

2014-01-01

428

Haootia quadriformis n. gen., n. sp., interpreted as a muscular cnidarian impression from the Late Ediacaran period (approx. 560 Ma).  

PubMed

Muscle tissue is a fundamentally eumetazoan attribute. The oldest evidence for fossilized muscular tissue before the Early Cambrian has hitherto remained moot, being reliant upon indirect evidence in the form of Late Ediacaran ichnofossils. We here report a candidate muscle-bearing organism, Haootia quadriformis n. gen., n. sp., from approximately 560 Ma strata in Newfoundland, Canada. This taxon exhibits sediment moulds of twisted, superimposed fibrous bundles arranged quadrilaterally, extending into four prominent bifurcating corner branches. Haootia is distinct from all previously published contemporaneous Ediacaran macrofossils in its symmetrically fibrous, rather than frondose, architecture. Its bundled fibres, morphology, and taphonomy compare well with the muscle fibres of fossil and extant Cnidaria, particularly the benthic Staurozoa. Haootia quadriformis thus potentially provides the earliest body fossil evidence for both metazoan musculature, and for Eumetazoa, in the geological record. PMID:25165764

Liu, Alexander G; Matthews, Jack J; Menon, Latha R; McIlroy, Duncan; Brasier, Martin D

2014-10-22

429

Chaulioleptos haywardi n. gen., n. sp. (Digenea: Sanguinicolidae) from Filimanus heptadactyla (Perciformes: Polynemidae) of Moreton Bay, Australia.  

PubMed

Here we describe the first species of sanguinicolid blood fluke (Trematoda: Digenea) from a polynemid fish. Chaulioleptos haywardi n. gen., n. sp. is described from Filimanus heptadactyla Cuvier, 1829 (Perciformes: Polynemidae), the sevenfinger threadfin from Sandgate, Moreton Bay (southeast Queensland, Australia). Chaulioleptos haywardi differs from existing sanguinicolid genera in the combined possession of the following 7 characters: 2 testes, an entirely postovarian uterus, a uterine chamber, separate genital pores, an H-shaped intestine with abbreviated anterior caeca, tegumental spines in incomplete ventro-marginal transverse rows that are continuous along the length of the body, and vitelline follicles that are tightly compacted and subsequently appear to form a solid branching mass occupying the area anterior to intestinal bifurcation and extending posteriorly to the level of the posterior margin of the anterior testis. Chaulioleptos haywardi is most closely related to Paracardicola Martin, 1960 and Adelomyllos Nolan and Cribb, 2004. PMID:16108558

Nolan, Matthew J; Cribb, Thomas H

2005-06-01

430

Morphology, ultrastructure and life cycle of Vitrella brassicaformis n. sp., n. gen., a novel chromerid from the Great Barrier Reef.  

PubMed

Chromerida are photoautotrophic alveolates so far only isolated from corals in Australia. It has been shown that these secondary plastid-containing algae are closely related to apicomplexan parasites and share various morphological and molecular characters with both Apicomplexa and Dinophyta. So far, the only known representative of the phylum was Chromera velia. Here we provide a formal description of another chromerid, Vitrella brassicaformis gen. et sp. nov., complemented with a detailed study on its ultrastructure, allowing insight into its life cycle. The novel alga differs significantly from the related chromerid C. velia in life cycle, morphology as well as the plastid genome. Analysis of photosynthetic pigments on the other hand demonstrate that both chromerids lack chlorophyll c, the hallmark of phototrophic chromalveolates. Based on the relatively high divergence between C. velia and V. brassicaformis, we propose their classification into distinct families Chromeraceae and Vitrellaceae. Moreover, we predict a hidden and unexplored diversity of the chromerid algae. PMID:22055836

Oborník, Miroslav; Modrý, David; Lukeš, Martin; Cernotíková-St?íbrná, Eva; Cihlá?, Jaromír; Tesa?ová, Martina; Kotabová, Eva; Vancová, Marie; Prášil, Ond?ej; Lukeš, Julius

2012-03-01

431

Third-Party Evaluation of Petro Tex Hydrocarbons, LLC, ReGen Lubricating Oil Re-refining Process  

SciTech Connect

This report presents an assessment of market, energy impact, and utility of the PetroTex Hydrocarbons, LLC., ReGen process for re-refining used lubricating oil to produce Group I, II, and III base oils, diesel fuel, and asphalt. PetroTex Hydrocarbons, LLC., has performed extensive pilot scale evaluations, computer simulations, and market studies of this process and is presently evaluating construction of a 23 million gallon per year industrial-scale plant. PetroTex has obtained a 30 acre site in the Texas Industries RailPark in Midlothian Texas. The environmental and civil engineering assessments of the site are completed, and the company has been granted a special use permit from the City of Midlothian and air emissions permits for the Texas Commission on Environmental Quality.

Compere, A L [ORNL; Griffith, William {Bill} L [ORNL

2009-04-01

432

A new genus of Strepsiptera, Rozenia gen. n. (Stylopidae), a parasite of bee genera Acamptopoeum and Calliopsis (Andrenidae, Panurginae, Calliopsini)  

PubMed Central

Abstract A new Strepsiptera genus from South America is described, Rozenia gen. n., with three new species: Rozenia calliopsidis sp. n. (type species), Rozenia peruana sp. n. and Rozenia platicephala sp. n. These three new species are parasites of bees belonging to the tribe Calliopsini (Andrenidae, Panurginae). Rozenia calliopsidis sp. n. is a parasite of the bee genus Calliopsis Smith, 1853 and Rozenia peruana sp. n. and Rozenia platicephala sp. n. are parasites of the bee genus Acamptopoeum Cockerell, 1905. Diagnoses and descriptions of female puparia are presented for all three species. Diagnoses and descriptions of first instars (triungulinids) are presented for Rozenia calliopsidis sp. n. and Rozenia platicephala sp. n. The first case of increased number of setae on the body of the first instars and augmentation of chaetotaxy of Strepsiptera are discussed. PMID:25349488

Straka, Jakub; J?zová, Katerina; Batelka, Jan

2014-01-01

433

Characterization of Some Actinomyces-Like Isolates from Human Clinical Sources: Description of Varibaculum cambriensis gen. nov., sp. nov.  

PubMed Central

Fifteen strains of an anaerobic, catalase-negative, gram-positive diphtheroid-shaped bacterium recovered from human sources were characterized by phenotypic and molecular chemical and molecular genetic methods. The unidentified bacterium showed some resemblance to Actinomyces species and related taxa, but biochemical testing, polyacrylamide gel electrophoresis analysis of whole-cell proteins, and amplified 16S ribosomal DNA restriction analysis indicated the strains were distinct from all currently named Actinomyces species and related taxa. Comparative 16S rRNA gene sequencing studies showed that the bacterium represents a hitherto-unknown phylogenetic line that is related to but distinct from Actinomyces, Actinobaculum, Arcanobacterium, and Mobiluncus. We propose, on the basis of phenotypic and phylogenetic evidence, that the unknown bacterium from human clinical specimens should be classified as a new genus and species, Varibaculum cambriensis gen. nov., sp. nov. The type strain of Varibaculum cambriensis sp. nov. is CCUG 44998T = CIP 107344T. PMID:12574260

Hall, Val; Collins, Matthew D.; Lawson, Paul A.; Hutson, Roger A.; Falsen, Enevold; Inganas, Elisabeth; Duerden, Brian

2003-01-01

434

Enhanced/synthetic vision and head-worn display technologies for terminal maneuvering area NextGen operations  

NASA Astrophysics Data System (ADS)

NASA is researching innovative technologies for the Next Generation Air Transportation System (NextGen) to provide a "Better-Than-Visual" (BTV) capability as adjunct to "Equivalent Visual Operations" (EVO); that is, airport throughputs equivalent to that normally achieved during Visual Flight Rules (VFR) operations rates with equivalent and better safety in all weather and visibility conditions including Instrument Meteorological Conditions (IMC). These new technologies build on proven flight deck systems and leverage synthetic and enhanced vision systems. Two piloted simulation studies were conducted to access the use of a Head-Worn Display (HWD) with head tracking for synthetic and enhanced vision systems concepts. The first experiment evaluated the use a HWD for equivalent visual operations to San Francisco International Airport (airport identifier: KSFO) compared to a visual concept and a head-down display concept. A second experiment evaluated symbology variations under different visibility conditions using a HWD during taxi operations at Chicago O'Hare airport (airport identifier: KORD). Two experiments were conducted, one in a simulated San Francisco airport (KSFO) approach operation and the other, in simulated Chicago O'Hare surface operations, evaluating enhanced/synthetic vision and head-worn display technologies for NextGen operations. While flying a closely-spaced parallel approach to KSFO, pilots rated the HWD, under low-visibility conditions, equivalent to the out-the-window condition, under unlimited visibility, in terms of situational awareness (SA) and mental workload compared to a head-down enhanced vision system. There were no differences between the 3 display concepts in terms of traffic spacing and distance and the pilot decision-making to land or go-around. For the KORD experiment, the visibility condition was not a factor in pilot's rating of clutter effects from symbology. Several concepts for enhanced implementations of an unlimited field-of-regard BTV concept for low-visibility surface operations were determined to be equivalent in pilot ratings of efficacy and usability.

Arthur, Jarvis J., III; Prinzel, Lawrence J., III; Williams, Steven P.; Bailey, Randall E.; Shelton, Kevin J.; Norman, R. Mike

2011-06-01

435

Evaluating the Suitability for CO2 Storage at the FutureGen 2.0 Site, Morgan County, Illinois, USA  

SciTech Connect

FutureGen 2.0 site will be the first near-zero emission power plant with fully integrated long-term storage in a deep, non-potable saline aquifer in the United States. The proposed FutureGen 2.0 CO2 storage site is located in northeast Morgan County, Illinois, U.S.A., forty-eight kilometres from the Meredosia Energy Center where a large-scale oxy-combustion demonstration will be conducted. The demonstration will involve > 90% carbon capture, which will produce more than one million metric tons (MMT) of CO2 per year. The CO2 will be compressed at the power plant and transported via pipeline to the storage site. To examine CO2 storage potential of the site, a 1,467m characterization well (FGA#1) was completed in December 2011. The target reservoir for CO2 storage is the Mt. Simon Sandstone and Elmhurst Sandstone Member of the lower Eau Claire Formation for a combined thickness of 176 m. Confining beds of the overlying Lombard and Proviso Members (upper Eau Claire Formation) reach a thickness of 126 m. Characterization of the target injection zone and the overlying confining zone was based on wellbore data, cores, and geophysical logs, along with surface geophysical (2-D seismic profiles, magnetic and gravity), and structural data collected during the initial stage of the project . Based on this geological model, 3D simulations of CO2 injection and redistribution were conducted using STOMP-CO2, a multiphase flow and transport simulator. After this characterization stage, it appears that the injection site is a suitable geologic system for CO2 sequestration and that the injection zone is sufficient to receive up to 33 MMT of CO2 at a rate of 1.1 MMT/yr. GHGT-11 conference

Bonneville, Alain; Gilmore, Tyler J.; Sullivan, E. C.; Vermeul, Vincent R.; Kelley, Mark E.; White, Signe K.; Appriou, Delphine; Bjornstad, Bruce N.; Gerst, Jacqueline L.; Gupta, Neeraj; Horner, Jacob A.; McNeil, Caitlin; Moody, Mark A.; Rike, William M.; Spane, Frank A.; Thorne, Paul D.; Zeller, Evan R.; Zhang, Z. F.; Hoffman, Jeffrey; Humphreys, Kenneth K.

2013-08-05

436

Fukuyoa paulensis gen. et sp. nov., a New Genus for the Globular Species of the Dinoflagellate Gambierdiscus (Dinophyceae)  

PubMed Central

The marine epiphytic dinoflagellate Gambierdiscus is a toxicologically important genus responsible for ciguatera fish poisoning, the principal cause of non-bacterial illness associated with fish consumption. The genus currently contains species exhibiting either globular or anterior-posteriorly compressed morphologies with marked differences in cell shape and plate arrangement. Here we report a third globular, epiphytic and tychoplanktonic species from the coasts of Ubatuba, Brazil. The new species can be distinguished from G. yasumotoi and G. ruetzleri by its broader first apical plate that occupies a larger portion of the epitheca. Accordingly, phylogenetic trees from small subunit (SSU) and large subunit (LSU) ribosomal DNA sequences also showed strongly supported separation of the new species from the G. yasumotoi / G. ruetzleri group albeit with short distance. The molecular phylogenies, which included new sequences of the planktonic species Goniodoma polyedricum, further indicated that the globular species of Gambierdiscus formed a tight clade, clearly separated (with strong bootstrap support) from the clade of lenticular species including the type for Gambierdiscus. The morphological and molecular data in concert support the split of Gambierdiscus sensu lato into two genera. Gambierdiscus sensu stricto should be reserved for the species with lenticular shapes, highly compressed anterioposteriorly, with short-shank fishhook apical pore plate, large 2' plate, low and ascending cingular displacement, and pouch-like sulcal morphology. The new genus name Fukuyoa gen. nov. should be applied to the globular species, slightly laterally compressed, with long-shank fishhook apical pore plate, large 1' plate, greater and descending cingular displacement, and not pouch-like vertically-oriented sulcal morphology. Fukuyoa contains the new species Fukuyoa paulensis gen. et sp. nov., and F. yasumotoi comb. nov. and F. ruetzleri comb. nov. PMID:25831082

Gómez, Fernando; Qiu, Dajun; Lopes, Rubens M.; Lin, Senjie

2015-01-01

437

Enhanced/Synthetic Vision and Head-Worn Display Technologies for Terminal Maneuvering Area NextGen Operations  

NASA Technical Reports Server (NTRS)

NASA is researching innovative technologies for the Next Generation Air Transportation System (NextGen) to provide a "Better-Than-Visual" (BTV) capability as adjunct to "Equivalent Visual Operations" (EVO); that is, airport throughputs equivalent to that normally achieved during Visual Flight Rules (VFR) operations rates with equivalent and better safety in all weather and visibility conditions including Instrument Meteorological Conditions (IMC). These new technologies build on proven flight deck systems and leverage synthetic and enhanced vision systems. Two piloted simulation studies were conducted to access the use of a Head-Worn Display (HWD) with head tracking for synthetic and enhanced vision systems concepts. The first experiment evaluated the use a HWD for equivalent visual operations to San Francisco International Airport (airport identifier: KSFO) compared to a visual concept and a head-down display concept. A second experiment evaluated symbology variations under different visibility conditions using a HWD during taxi operations at Chicago O'Hare airport (airport identifier: KORD). Two experiments were conducted, one in a simulated San Francisco airport (KSFO) approach operation and the other, in simulated Chicago O'Hare surface operations, evaluating enhanced/synthetic vision and head-worn display technologies for NextGen operations. While flying a closely-spaced parallel approach to KSFO, pilots rated the HWD, under low-visibility conditions, equivalent to the out-the-window condition, under unlimited visibility, in terms of situational awareness (SA) and mental workload compared to a head-down enhanced vision system. There were no differences between the 3 display concepts in terms of traffic spacing and distance and the pilot decision-making to land or go-around. For the KORD experiment, the visibility condition was not a factor in pilot's rating of clutter effects from symbology. Several concepts for enhanced implementations of an unlimited field-of-regard BTV concept for low-visibility surface operations were determined to be equivalent in pilot ratings of efficacy and usability.

Arthur, Jarvis J., III; Prinzell, Lawrence J.; Williams, Steven P.; Bailey, Randall E.; Shelton, Kevin J.; Norman, R. Mike

2011-01-01

438

Abstract. The contentious and litigious history associated with managing Northern Goshawks (Accipiter gen-tilis) has focused much research attention toward understanding this species' life history. Results from these  

E-print Network

Words: Accipiter gentilis, goshawk ecology, goshawk management, Northern Goshawk. ECOLOGÍA DEL GAVILÁN (Accipiter gen- tilis) has focused much research attention toward understanding this species' life history CONSERVACIÓN Resumen. La contenciosa y discordante historia asociada al manejo del Gavilán Azor (Accipiter

439

Ge Migration Effect of Si\\/Gen\\/Si(100) Heterostructure Films Probed by Grazing Incidence Fluorescence X-Ray Absorption Fine Structure  

Microsoft Academic Search

The local structures of Si(20 monolayer)\\/Gen\\/Si(100) (n=1, 2, 4 and 8 monolayer) heterostructure films grown by molecular beam epitaxy (MBE) at 400 °C are investigated by grazing incidence fluorescence x-ray absorption fine structure. The Ge K-edge EXAFS analysis shows that the local structure of Ge atoms in the 1 or 2 monolayer (ML) Ge heterostructure film where the Ge atoms

Zhiyun Pan; Hiroyuki Oyanagi; Zhihu Sun; Zhi Xie; Jiangwei Fan; Shiqiang Wei

2007-01-01

440

Emwadea microcarpa gen. et sp. nov.—anatomically preserved araucarian seed cones from the Winton Formation (late Albian), western Queensland, Australia  

Microsoft Academic Search

Dettmann, M.E., Clifford, H.T., Peters, M., iFirst article. Emwadea microcarpa gen. et sp. nov.—anatomically preserved araucarian seed cones from the Winton Formation (late Albian), western Queensland, Australia. Alcheringa, 1–21. ISSN 0311-5518. A new genus and species, Emwadea microcarpa Dettmann, Clifford & Peters, is established for ovulate\\/seed cones with helically arranged cone scales bearing a centrally positioned, inverted ovule from the

Mary E. Dettmann; H. Trevor Clifford; Mark Peters

2012-01-01

441

On the Emsian (Early Devonian) arthropods of the Rhenish Slate Mountains: 4. The eurypterids Alkenopterus and Vinetopterus n. gen. (Arthropoda: Chelicerata)  

Microsoft Academic Search

Recently collected material of Early Devonian eurypterids (Chelicerata: Eurypterida) from the Rhenish Slate Mountains is described\\u000a and compared to the reexamined type material ofDrepanopterus struvei\\u000a Størmer, 1974 andAlkenopterus brevitelson\\u000a Størmer, 1974, both from the Emsian of Alken an der Mosel.Vinetopterus martini n. gen., n. sp., from Siegenian strata of the newly detected eurypterid localities Burglahr and Hombach in the Westerwald,

Markus Poschmann; O. Erik Tetlie

2004-01-01

442

VARIA?'ES GEN…TICAS PARA CARACTER?STICAS DO SISTEMA RADICULAR DE MUDAS DE BARU (Dipteryx alata Vog.)1  

Microsoft Academic Search

RESUMO ñ Este estudo objetivou estimar os par‚metros genØticos relativos ‡ qualidade do sistema radicular de mudas de baru. Para isso, foram utilizadas mudas provenientes de sementes colhidas em 21 Ærvores-matriz de polinizaÁªo livre no MunicÌpio de CapinÛpolis, Minas Gerais. Quarenta e cinco dias apÛs a germinaÁªo, as imagens das raÌzes de 20 mudas de cada progŒnie foram obtidas por

Adelson Nascimento Oliveira; Ana Carolina da Silva; Carlos da Silva Rosado; Crespo Rodrigues

2006-01-01

443

Evaluation of Performance of the Gen-Probe Human Immunodeficiency Virus Type 1 Viral Load Assay Using Primary Subtype A, C, and D Isolates from Kenya  

PubMed Central

Accurate and sensitive quantification of human immunodeficiency virus type 1 (HIV-1) RNA has been invaluable as a marker for disease prognosis and for clinical monitoring of HIV-1 disease. The first generation of commercially available HIV-1 RNA tests were optimized to detect the predominant HIV-1 subtype found in North America and Europe, subtype B. However, these tests are frequently suboptimal in detecting HIV-1 genetic forms or subtypes found in other parts of the world. The goal of the present study was to evaluate the performance of a new viral load assay with non-subtype B viruses. A transcription-mediated amplification method for detection and quantitation of diverse HIV-1 subtypes, called the Gen-Probe HIV-1 viral load assay, is under development. In this study we examined the performance of the Gen-Probe HIV-1 viral load assay relative to that of the commonly used commercial HIV-1 RNA assays using a panel of primary isolates from Kenya. For comparison, we included several subtype B cloned viruses, and we quantified each virus using an in-house quantitative-competitive reverse transcriptase PCR (QC-RT-PCR) method and gagp24 antigen capture. The Gen-Probe HIV-1 viral load assay and a version of the Roche AMPLICOR HIV-1 MONITOR test (version 1.5) that was designed to detect a broader range of subtypes were both sensitive for the quantification of Kenyan primary isolates, which represented subtype A, C, and D viruses. The Gen-Probe HIV-1 viral load assay was more sensitive for the majority of viruses than the Roche AMPLICOR HIV-1 MONITOR test version 1.0, the Bayer Quantiplex HIV RNA 3.0 assay, or a QC-RT-PCR method in use in our laboratory, suggesting that it provides a useful method for quantifying HIV-1 RNAs from diverse parts of the world, including Africa. PMID:10878065

Emery, Sandra; Bodrug, Sharon; Richardson, Barbra A.; Giachetti, Cristina; Bott, Martha A.; Panteleeff, Dana; Jagodzinski, Linda L.; Michael, Nelson L.; Nduati, Ruth; Bwayo, Job; Kreiss, Joan K.; Overbaugh, Julie

2000-01-01

444

Loricifera from the deep sea at the Galápagos Spreading Center, with a description of Spinoloricus turbatio gen. et sp. nov. (Nanaloricidae)  

Microsoft Academic Search

Specimens of a new species of Loricifera, Spinoloricus turbatio gen. et sp. nov., have been collected at the Galápagos Spreading Center (GSC) during the cruise SO 158, which is a part of\\u000a the MEGAPRINT project. The new genus is positioned in the family Nanaloricidae together with the three already described genera\\u000a Nanaloricus, Armorloricus and Phoeniciloricus. The postlarvae and adults of

Iben Heiner; Birger Neuhaus

2007-01-01

445

GenExP, un logiciel simulateur de paysages agricoles pour l'etude de la diffusion de transg`enes  

E-print Network

dispersion des pollens et des graines d'OGM `a diff´erentes ´echelles. Abstract. The software GenExP allows ou les transports m´ecaniques, des transg`enes issus des cultures g´en´etiquement modifi´ees (OGM'offre "Impact des OGM" du Minist`ere de la recherche (2002). Nous avons d´evelopp´e un simulateur de paysages

Boyer, Edmond

446

A new African soft scale genus, Pseudocribrolecanium gen. nov. (Hemiptera: Coccoidea: Coccidae), erected for two species, including the citrus pest P. andersoni (Newstead) comb. nov  

PubMed Central

A new African genus of soft scale insects, Pseudocribrolecanium gen. nov. is erected to accommodate Akermes colae Green & Laing and Cribrolecanium andersoni (Newstead). The adult females and first-instar nymphs of the two species are redescribed and illustrated. Taxonomic keys to separate the adult females and first-instar nymphs are provided. The affinity of Pseudocribrolecanium with the tribe Paralecaniini in the subfamily Coccinae is discussed. PMID:19537997

Kondo, Takumasa

2006-01-01

447

Intraspecific Variation in Small-Subunit rRNA Sequences in GenBank: Why Single Sequences May Not Adequately Represent Prokaryotic Taxa  

Microsoft Academic Search

Small-subunit rRNA (SSU rRNA) sequencing is a powerful tool to detect, identify, and classify prokaryotic organisms, and there is currently an explosion of SSU rRNA sequencing in the microbiology community. We report unexpectedly high levels of intraspecific variation (within and between strains) of prokaryote SSU rRNA sequences deposited in GenBank. A total of 82% of the prokaryote species with two

REBECCA A. CLAYTON; GRANGER SUTTON; PAUL S. HINKLE; CAROL BULT

448

AtGEN1 and AtSEND1, Two Paralogs in Arabidopsis, Possess Holliday Junction Resolvase Activity1[W][OPEN  

PubMed Central

Holliday junctions (HJs) are physical links between homologous DNA molecules that arise as central intermediary structures during homologous recombination and repair in meiotic and somatic cells. It is necessary for these structures to be resolved to ensure correct chromosome segregation and other functions. In eukaryotes, including plants, homologs of a gene called XPG-like endonuclease1 (GEN1) have been identified that process HJs in a manner analogous to the HJ resolvases of phages, archaea, and bacteria. Here, we report that Arabidopsis (Arabidopsis thaliana), a eukaryotic organism, has two functional GEN1 homologs instead of one. Like all known eukaryotic resolvases, AtGEN1 and Arabidopsis single-strand DNA endonuclease1 both belong to class IV of the Rad2/XPG family of nucleases. Their resolvase activity shares the characteristics of the Escherichia coli radiation and UV sensitive C paradigm for resolvases, which involves resolving HJs by symmetrically oriented incisions in two opposing strands. This leads to ligatable products without the need for further processing. The observation that the sequence context influences the cleavage by the enzymes can be interpreted as a hint for the existence of sequence specificity. The two Arabidopsis paralogs differ in their preferred sequences. The precise cleavage positions observed for the resolution