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Sample records for grifola frondosa solving

  1. Isolation, purification and antioxidant activities of polysaccharides from Grifola frondosa.

    PubMed

    Chen, Gui-tang; Ma, Xue-mei; Liu, Sheng-to; Liao, Yan-li; Zhao, Guo-qang

    2012-06-01

    The crude polysaccharides (GFP) were isolated from the fruiting bodies of Grifola frondosa and purified by DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography in that order. Three main fractions, GFP-1, GFP-2 and GFP-3, were obtained through the isolation and purification steps. Then the antioxidant activities of these three fractions were investigated in vitro. The results showed that GFP-1, GFP-2 and GFP-3 possessed significant inhibitory effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical; their reducing power, ferrous ions chelating effect and the inhibition ability of the rat liver lipid oxidation where also strong. These results suggest that G. frondosa polysaccharides could be a suitable natural antioxidant and may be the functional foods for humans. PMID:24750604

  2. Immunomodulatory activity of macromolecular polysaccharide isolated from Grifola frondosa.

    PubMed

    Ma, Xiao-Lei; Meng, Meng; Han, Li-Rong; Li, Zheng; Cao, Xiao-Hong; Wang, Chun-Ling

    2015-12-01

    The present study was designed to evaluate the immune-modulating effects of the polysaccharide from Grifola frondosa (GFP) by using mouse peritoneal macrophage and cytoxan (CTX) induced immunosuppression models. Our results from the phagocytotic and mononuclear phagocytic system function assays showed that GFP-A (one component from GFP) stimulated the phagocytosis of the phagocytes. The splenocyte proliferation assay showed that GFP-A acted the effect combing ConA or LPS in splenocyte proliferation. The results showed that GFP-A increased indices of thymus and spleen, the levels of LDH and ACP in the spleen, the mRNA levels of IL-1β, IL-2, IL-6 and IFN-γ in splenocyte. And GFP-A also significantly increased the expression of CD4(+) and CD8(+) splenic T lymphocytes, which were suppressed by the CTX in peripheral blood. In conclusion, our results indicate that the GFP-A is involved in immunomodulatory effects leading to its modulatory effects on immunosuppression. PMID:26721709

  3. Antioxidant properties and antioxidant compounds of various extracts from the edible basidiomycete Grifola frondosa (Maitake).

    PubMed

    Yeh, Jan-Ying; Hsieh, Li-Hui; Wu, Kaun-Tzer; Tsai, Cheng-Fang

    2011-01-01

    Grifola frondosa is an edible mushroom currently available in Taiwan. Ethanolic, cold-water and hot-water extracts were prepared and their antioxidant properties were investigated. At 1 mg/mL, G. frondosa T1 and T2 cold-water extracts showed high reducing powers of 1.02 and 0.50, respectively. Chelating abilities on ferrous ions of G. frondosa T1 and T2 were higher for cold-water extracts than for ethanolic and hot-water extracts. For the scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, G. frondosa T1 and T2 extracts were effective in the following order: ethanolic > hot-water > cold-water. The G. frondosa hot-water extract showed high scavenging ability on superoxide anions. Total phenols, flavonoids, ascorbic acid and α-tocopherol are the major antioxidant components found in the various G. frondosa extracts. Based on EC(50) values (<20 mg/mL) obtained, the various extracts from G. frondosa investigated in this study display potent antioxidative properties. PMID:21499220

  4. The Mechanisms Underlying the Hypolipidaemic Effects of Grifola frondosa in the Liver of Rats.

    PubMed

    Ding, Yinrun; Xiao, Chun; Wu, Qingping; Xie, Yizhen; Li, Xiangmin; Hu, Huiping; Li, Liangqiu

    2016-01-01

    The present study investigated the hypolipidaemic effects of Grifola frondosa and its regulation mechanism involved in lipid metabolism in liver of rats fed a high-cholesterol diet. The body weights and serum lipid levels of control rats, of hyperlipidaemic rats, and of hyperlipidaemic rats treated with oral G. frondosa were determined. mRNA expression and concentration of key lipid metabolism enzymes were investigated. Serum cholesterol, triacylglycerol, and low-density lipoprotein cholesterol levels were markedly decreased in hyperlipidaemic rats treated with G. frondosa compared with untreated hyperlipidaemic rats. mRNA expression of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), acyl-coenzyme A: cholesterol acyltransferase (ACAT2), apolipoprotein B (ApoB), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC1) were significantly down-regulated, while expression of cholesterol 7-alpha-hydroxylase (CYP7A1) was significantly up-regulated in the livers of treated rats compared with untreated hyperlipidaemic rats. The concentrations of these enzymes also paralleled the observed changes in mRNA expression. Two-dimensional polyacrylamide gel electrophoresis (2-DE) and Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) were used to identify 20 proteins differentially expressed in livers of rats treated with G. frondosa compared with untreated hyperlipidemic rats. Of these 20 proteins, seven proteins were down-regulated, and 13 proteins were up-regulated. These findings indicate that the hypolipidaemic effects of G. frondosa reflected its modulation of key enzymes involved in cholesterol and triacylglycerol biosynthesis, absorption, and catabolic pathways. G. frondosa may exert anti-atherosclerotic effects by inhibiting LDL oxidation through down-regulation and up-regulating proteins expression in the liver of rats. Therefore, G. frondosa may produce both hypolipidaemic and anti-atherosclerotic effects, and potentially

  5. The Mechanisms Underlying the Hypolipidaemic Effects of Grifola frondosa in the Liver of Rats

    PubMed Central

    Ding, Yinrun; Xiao, Chun; Wu, Qingping; Xie, Yizhen; Li, Xiangmin; Hu, Huiping; Li, Liangqiu

    2016-01-01

    The present study investigated the hypolipidaemic effects of Grifola frondosa and its regulation mechanism involved in lipid metabolism in liver of rats fed a high-cholesterol diet. The body weights and serum lipid levels of control rats, of hyperlipidaemic rats, and of hyperlipidaemic rats treated with oral G. frondosa were determined. mRNA expression and concentration of key lipid metabolism enzymes were investigated. Serum cholesterol, triacylglycerol, and low-density lipoprotein cholesterol levels were markedly decreased in hyperlipidaemic rats treated with G. frondosa compared with untreated hyperlipidaemic rats. mRNA expression of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), acyl-coenzyme A: cholesterol acyltransferase (ACAT2), apolipoprotein B (ApoB), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC1) were significantly down-regulated, while expression of cholesterol 7-alpha-hydroxylase (CYP7A1) was significantly up-regulated in the livers of treated rats compared with untreated hyperlipidaemic rats. The concentrations of these enzymes also paralleled the observed changes in mRNA expression. Two-dimensional polyacrylamide gel electrophoresis (2-DE) and Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) were used to identify 20 proteins differentially expressed in livers of rats treated with G. frondosa compared with untreated hyperlipidemic rats. Of these 20 proteins, seven proteins were down-regulated, and 13 proteins were up-regulated. These findings indicate that the hypolipidaemic effects of G. frondosa reflected its modulation of key enzymes involved in cholesterol and triacylglycerol biosynthesis, absorption, and catabolic pathways. G. frondosa may exert anti-atherosclerotic effects by inhibiting LDL oxidation through down-regulation and up-regulating proteins expression in the liver of rats. Therefore, G. frondosa may produce both hypolipidaemic and anti-atherosclerotic effects, and potentially

  6. Biological activities of Fructus arctii fermented with the basidiomycete Grifola frondosa.

    PubMed

    Kim, Jin-Hwa; Bae, Jun-Tae; Song, Min-Hyeon; Lee, Geun-Su; Choe, Soo-Young; Pyo, Hyeong-Bae

    2010-12-01

    Fructus arctii extract containing phenolic glycosides was cultured with Grifola frondosa mycelia to produce β-glucosidase and its biological activities were studied. This β-glucosidase converted the glycosides (arctiin and caffeic acid derivatives) into aglycones (arctigenin and caffeic acid). Fermented Fructus arctii extract (G-FAE) with G. frondosa had antioxidant and 5-lipoxygenase inhibitory activities. The photoprotective potential of G-FAE was tested in human dermal fibroblasts (HDF) exposed to ultra-violet A (UVA). It was revealed that G-FAE had an inhibitory effect on human interstitial collagenase (matrix metalloproteinase, MMP-1) expression in UVA-irradiated HDF. The treatment of UVA-irradiated HDF with G-FAE resulted in a dose-dependent decrease in the expression level of MMP-1 mRNA. G-FAE also showed notable stimulation of collagen biosynthetic activity for fibroblasts. These diverse functionalities suggest that G-FAE could be a promising cosmetic ingredient. PMID:21191759

  7. Physical characteristics and antioxidant effect of polysaccharides extracted by boiling water and enzymolysis from Grifola frondosa.

    PubMed

    Fan, Yina; Wu, Xiangyang; Zhang, Min; Zhao, Ting; Zhou, Ye; Han, Liang; Yang, Liuqing

    2011-06-01

    Grifola frondosa has been widely consumed in China and other Asian countries. Recent studies on G. frondosa have focused on the activities of polysaccharides extracted by water, and the activities of polysaccharides extracted by enzymolysis have not been studied. In this work, the relationship between the physical properties and antioxidant activity of polysaccharides extracted from G. frondosa by boiling water and enzymolysis was studied. Five polysaccharide extracts from the fruit body of G. frondosa were prepared by different extracting methods including boiling water, single enzyme enzymolysis with three different single enzymes (cellulose, pectinase, and pancreatin), and combined enzyme enzymolysis (cellulose:pectinase:pancreatin; 2:2:1). Characteristics such as the viscosity, Mw, polysaccharide content, protein content, infrared spectra, and antioxidant activities of the extracts were evaluated. The highest antioxidant activity was exhibited by the extracts prepared by combined enzyme extraction. The correlation analysis between antioxidant activity and polysaccharide content, protein content, Mw or viscosity indicated that the Mw had a more important role in antioxidant activity. Overall, the results indicate that the combined enzyme polysaccharide extracts can be developed as a new potential natural antioxidant. PMID:21458482

  8. Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake).

    PubMed

    Montoya, Sandra; Orrego, Carlos Eduardo; Levin, Laura

    2012-04-01

    Cultivation of specialty mushrooms on lignocellulosic wastes represents one of the most economical organic recycling processes. Compared with other cultivated mushrooms, very little is known about the nature of the lignocellulolytic enzymes produced by the edible and medicinal fungus Grifola frondosa, the parameters affecting their production, and enzyme activity profiles during different stages of the developmental cycle. In this work we investigated the enzymes that enable G. frondosa, to colonize and deconstruct two formulations based on industrial lignocellulosic by-products. G. frondosa degraded both substrates (oak-sawdust plus corn bran, and oak/corn bran supplemented with coffee spent-ground) decreasing 67 and 50% of their lignin content, along with 44 and 37% of the polysaccharides (hemicellulose and cellulose) respectively. 35.3% biological efficiency was obtained when using oak sawdust plus corn bran as substrate. Coffee spent-ground addition inhibited mushroom production, decreased growth, xylanase and cellulase activities. However, taking into account that G. frondosa successfully colonized this residue; this substrate formula might be considered for its growth and medicinal polysaccharide production. Although G. frondosa tested positive for Azure B plate degradation, a qualitative assay for lignin-peroxidase, attempts to detect this activity during solid state fermentation were unsuccessful. Enzyme activities peaked during colonization but declined drastically during fruiting body formation. Highest activities achieved were: endoglucanase 12.3, exoglucanase 16.2, β-glucosidase 2.3, endoxylanase 20.3, amylase 0.26, laccase 14.8 and Mn-peroxidase 7.4 U/g dry substrate. PMID:22805935

  9. Structural characterization and antiviral activity of a novel heteropolysaccharide isolated from Grifola frondosa against enterovirus 71.

    PubMed

    Zhao, Chao; Gao, Luying; Wang, Chunyang; Liu, Bin; Jin, Yu; Xing, Zheng

    2016-06-25

    A novel heteropolysaccharide from Grifola frondosa mycelia was extracted and purified using DEAE Sephadex A-50 and Sephadex G-200 chromatography. Fourier transform infrared (FT-IR) spectroscopy and nuclear magnetic resonance ((1)H NMR and (13)C NMR) spectroscopy were used to decipher the structure of the purified G. frondosa polysaccharide (GFP1). Chemical and spectral analysis revealed that GFP1, with an average molecular weight of 40.5kDa, possessed a 1,6-β-d-glucan backbone with a single 1,3-α-d-fucopyranosyl side-branching unit. Enterovirus 71 (EV71) is the causative pathogen of hand-foot-and-mouth disease. GFP1 was tested for its anti-EV71 activity in cultured cells, which showed that EV71 viral replication was blocked and viral VP1 protein expression and genomic RNA synthesis were suppressed. Moreover, GFP1 exhibited apoptotic and other activities by suppressing the EV71-induced caspase-3 cleavage and IκBα down regulation. Our results demonstrate that the novel G. frondosa polysaccharide has antiviral activity, which could be valuable as a potentially new anti-EV71 therapeutic compound. PMID:27083830

  10. [Experimental study on intervention effect of Grifola frondosa on nonalcoholic steatohepatitis].

    PubMed

    Dai, Xian-wei; Chen, Zhi-yun; Yan, Mao-xiang; He, Bei-hui

    2015-05-01

    To study the preventive effect of Grifola frondosa on nonalcoholic steatohepatitis (NASH). The rat model of NASH was established by feeding high-fat diets for 12 weeks and intervened with 0.5 g · kg(-1) · d(-1) and 1.0 g · kg(-1) · d(-1) of C. frondosa powder suspensions. The degrees of hepatocyte fatty degeneration and inflammation were observed under the optical microscope with routine HE staining. The NAFLD activity scores (NAS) were calculated. Serum ALT, AST and hepatic TG and CHOL were tested by the biochemical method. The hepatic MDA was examined by thiobarbituric acid method. The hepatic SOD was tested by the xanthine oxidase test. The hepatic GSH-PX activity was determined by the dithio-nitrobenzoic acid method. Hepatic TNF-α and IL-6 were detected by the enzyme-linked immunosorbent assay (ELISA). The NASH model group induced by high-fat diets showed higher hepatic NAS, ser- um ALT, AST, CHOL and hepatic TG, CHOL, MDA, TNF-α, IL-6 (P < 0.01 or P < 0.05) and lower serum TG and hepatic SOD, GSH-PX (P < 0.01, P < 0.05) than the normal control group. After being intervened with different doses of G. frondosa, the NASH group revealed significantly lower hepatic NAS, serum ALT and hepatic TG, CHOL, MDA, TNF-α and IL-6 (P < 0.05) and higher hepatic SOD, GSH-PX (P < 0.05) than the model group. G. frondosa may prevent the further development of NASH by improving the disorder of lipid metabolism in rats with NASH induced by high-fat diets, relieving the level of oxidative stress and reducing the generation of inflammatory cytokines. PMID:26323153

  11. Lignin degradation, ligninolytic enzymes activities and exopolysaccharide production by Grifola frondosa strains cultivated on oak sawdust.

    PubMed

    Fernandes, Nona A; Isikhuemhen, Omoanghe S; Ohimain, Elijah I

    2011-07-01

    Fourteen strains of Grifola frondosa (Dicks.) S. F. Gray, originating from different regions (Asia, Europe and North America) were tested for lignin degradation, ligninolytic enzyme activities, protein accumulation and exopolysaccharide production during 55 days of cultivation on oak sawdust. Lignin degradation varied from 2.6 to7.1 % of dry weight of the oak sawdust substrate among tested strains. The loss of dry matter in all screened fungi varied between 11.7 and 33.0%, and the amount of crude protein in the dry substrate varied between 0.94 to 2.55%. The strain, MBFBL 596, had the highest laccase activity (703.3 U/l), and the maximum peroxidase activity of 22.6 U/l was shown by the strain MBFBL 684. Several tested strains (MBFBL 21, 638 and 662) appeared to be good producers of exopolysaccharides (3.5, 3.5 and 3.2 mg/ml respectively). PMID:24031728

  12. Immunomodulatory activities of medicinal mushroom Grifola frondosa extract and its bioactive constituent.

    PubMed

    Wu, Shu-Jing; Lu, Tzy-Ming; Lai, Min-Nan; Ng, Lean-Teik

    2013-01-01

    Grifola frondosa (GF), a high value medicinal mushroom in China and Japan, is popularly consumed as traditional medicines and health foods, especially for enhancing immune functions. In this study, our aim was to examine the immunomodulatory activities of GF and its bioactive compound ergosterol peroxide (EPO) in lipopolysaccharide (LPS)-induced human monocytic (THP-1) cells. At low concentrations, EPO but not other extracts showed a full protection against LPS-induced cell toxicity. EPO significantly blocked MyD88 and VCAM-1 expression, and cytokine (IL-1β, IL-6 and TNF-α) production in LPS-stimulated cells. It also effectively inhibited NF-κB activation, which was further confirmed with siRNA treatment. These results conclude that EPO may play an important role in the immunomodulatory activity of GF through inhibiting the production of pro-inflammatory mediators and activation of NF-κB signaling pathway. PMID:23336512

  13. Extraction, identification and antimicrobial activity of a new furanone, grifolaone A, from Grifola frondosa.

    PubMed

    He, Xiaoyun; Du, Xuezhu; Zang, Xiangyun; Dong, Lili; Gu, Zijian; Cao, Liping; Chen, Dafu; Keyhani, Nemat O; Yao, Lindan; Qiu, Junzhi; Guan, Xiong

    2016-04-01

    A furanone (1), (S)-methyl 2-(2-hydroxy-3,4-dimethyl-5-oxo-2,5-dihydrofuran-2-yl)acetate, was isolated from the edible mushroom Grifola frondosa. Mass spectrometry and NMR analyses were used to elucidate the structure of this compound, and its absolute configuration was determined using circular dichroism spectroscopy. Compound 1 exhibited specific antifungal activity against the plant pathogens, Fusarium oxysporum, Gibberella zeae and Piricularia oryzae and the opportunistic human pathogen, Pseudallescheria boydii, resulting in minimum inhibitory concentration values of 2.5, 2.5, 1.25 and 0.15 μg/mL, respectively. In contrast, the furanone showed only weak activity towards Aspergillus spp., Candida albicans and several other fungal strains tested as well as no appreciable antibacterial activity. PMID:26374926

  14. Optimization of subcritical water extraction of polysaccharides from Grifola frondosa using response surface methodology

    PubMed Central

    Yang, Liuqing; Qu, Hongyuan; Mao, Guanghua; Zhao, Ting; Li, Fang; Zhu, Bole; Zhang, Bingtao; Wu, Xiangyang

    2013-01-01

    Background: This research is among the few that has been conducted on the feasibility of subcritical water extraction (SWE) as a rapid and efficient extraction tool for polysaccharides. Objective: The aim of the study was to extractand optimize the parameter conditions of SWE of polysaccharides from Grifola frondosa using response surface methodology. Materials and Methods: In the study, SWEwas applied to extractbioactive compounds from G. frondosa. A preliminary analysis was made on the physical properties and content determination of extracts using SWE and hot water extraction (HWE). Analysis of the sample residues and antioxidant activities of the polysaccharides extracted by SWE and HWE were then evaluated. Results: The optimal extraction conditions include: extraction temperature of 210°C, extraction time of 43.65 min and the ratio of water to raw material of 26.15:1. Under these optimal conditions, the experimental yield of the polysaccharides (25.1 ± 0.3%) corresponded with the mean value predicted by the model and two times more than the mean value obtained by the traditional HWE. The antioxidant activities of polysaccharides extracted by SWE were generally higher than those extracted by HWE. From the study, the SWE technology could be a time-saving, high yield, and bioactive technique for production of polysaccharides. PMID:23772107

  15. Antitumor and immunomodulatory activity of a water-soluble polysaccharide from Grifola frondosa.

    PubMed

    Mao, Guang-Hua; Ren, Yi; Feng, Wei-Wei; Li, Qian; Wu, Hui-Yu; Jin, Dun; Zhao, Ting; Xu, Cai-Quan; Yang, Liu-Qing; Wu, Xiang-Yang

    2015-12-10

    Grifola frondosa has long been known and respected as a medically important fungus. This study investigated the characterization, antitumor and immunomodulatory activity of a polysaccharide named GP11 purified from G. frondosa. The results revealed that GP11 was composed of → 1)-D-Manp-(6 →,→ 1)-D-Glcp-(4 →,→ 1)-D-Galp-(6 → and → 2,3,6)-D-Glcp-(1 →, with branches attached at O-2,3 of 1,2,3,6-linked Glcp residues and terminal T-Glcp. GP11 exhibited indirect cytotoxic activity against HepG-2 cells in vitro, and it significantly inhibited the growth of Heps cells in vivo. GP11 increased the relative thymus and spleen weights as well as serum tumor necrosis factor-alpha and interleukin-2 levels. GP11 stimulated tumoricidal activity and the production of nitric oxide (NO), TNF-α and interleukin-1β, and it also stimulated the protein expression of iNOS and mRNA expression of iNOS and TNF-α. TLR-4 is a potential receptor for GP11-mediated macrophage activation. The results suggested that the antitumor activity of GP11 may be due to the improvement of immune functions through the TLR-4-mediated up-regulation of NO and TNF-α. PMID:26428141

  16. Decolorization of synthetic dyes and biodegradation of bisphenol a by laccase from the edible mushroom, Grifola frondosa.

    PubMed

    Nitheranont, Thitinard; Watanabe, Akira; Suzuki, Toshisada; Katayama, Takeshi; Asada, Yasuhiko

    2011-01-01

    A major laccase isozyme from Grifola frondosa (Lac 1) was found to be effective for decolorizing of synthetic dyes and degrading of bisphenol A. The oxidative capability of Lac 1 toward synthetic dyes and bisphenol A was enhanced in the presence of the redox mediator, 1-hydroxybenzotriazole. The major product from the degradation of bisphenol A by Lac 1 was determined to be 4-isopropenylphenol. PMID:21897011

  17. A polysaccharide from Grifola frondosa relieves insulin resistance of HepG2 cell by Akt-GSK-3 pathway.

    PubMed

    Ma, Xiaolei; Zhou, Fuchuan; Chen, Yuanyuan; Zhang, Yuanyuan; Hou, Lihua; Cao, Xiaohong; Wang, Chunling

    2014-07-01

    Grifola frondosa is an important fungal research resource. However, there was little report about hyperglycemic activity of Grifola frondosa polysaccharide on insulin resistance in vitro. In this study, the hypoglycemic activity of a polysaccharide obtained from Grifola frondosa (GFP) on HepG2 cell and hpyerglycemic mechanism were investigated. The purity of the isolated polysaccharides was examined by HPLC. In this research, it was found that GFP enhanced the absorption of glucose of HepG2 cells in a dose dependent manner at 24 h of 30 ugmL⁻¹. GC-MS and FT-IR spectroscopy analysis results showed that glucose and galactose were the dominant monosaccharides in GFP and the major component of GFP was β-pyranoside. Western-blotting results showed that the HepG2 cell model treated with GFP activated the insulin receptor protein (IRS) in the cell membrane and increased phosphorylated-AktSer473 expression, which had an inhibition of glycogen synthase kinase (GSK-3). The down-regulation of GSK-3 stimulated synthesis of intracellular glycogen. The results above suggested that the GFP increased the metabolism of glucose and stimulated synthesis of intracellular glycogen through the Akt/GSK-3 pathway. PMID:24908430

  18. Experimental analysis on the main contents of Rhizoma gastrodiae extract and inter-transformation throughout the fermentation process of Grifola frondosa.

    PubMed

    Wang, Na; Wu, Tian-xiang; Zhang, Yong; Xu, Xiao-bao; Tan, Sha; Fu, Hong-Wei

    2013-03-01

    Gastrodin (GA), p-hydroxylbenzaldehyde (HBA), p-hydroxybenzyl alcohol (gastrodigenin, HA) and parishin not only are the major active ingredients of Rhizoma gastrodiae, but exist transformed relations from each other throughout the fermentation process of Grifola frondosa in this work. We had found that parishin (non-free gastrodin) almost could completely transformed into gastrodin (GA, free gastrodin) after R. gastrodiae alcohol extract was sterilized by moist heat (121 °C, 30 min), but before was added into submerged cultivation of G. frondosa. However, interestingly and importantly, gastrodin re-synthesized of parishin after R. gastrodiae alcohol extract's addition into submerged cultivation of G. frondosa. In addition, the reduction of p-hydroxylbenzaldehyde and p-hydroxybenzyl alcohol in G. frondosa fermentation process reconfirmed that the G. frondosa strain 51616 really could synthesize gastrodin into parishin by submerged fermentation. This paper firstly also reported G. frondosa's effects on R. gastrodiae. PMID:23435908

  19. Extracellular laccase produced by an edible basidiomycetous mushroom, Grifola frondosa: purification and characterization.

    PubMed

    Nitheranont, Thitinard; Watanabe, Akira; Asada, Yasuhiko

    2011-01-01

    A major laccase isozyme (Lac 1) was isolated from the culture fluid of an edible basidiomycetous mushroom, Grifola frondosa. Lac 1 was revealed to be a monomeric protein with a molecular mass of 71 kDa. The N-terminal amino acid sequence of Lac 1 was highly similar to those of laccases of some other white-rot basidiomycetes. Lac 1 showed the typical absorption spectrum of a copper-containing enzyme. The enzyme was stable in a wide pH range (4.0 to 10.0), and lost no activity up to 60 °C for 60 min. The optimal pH of the enzyme activity varied among substrates. The K(m) values of Lac 1 toward 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), 2,6-dimethoxyphenol, guaiacol, catechol, and 3,4-dihydroxy-L-phenylalanine were 0.0137 mM, 0.608 mM, 0.531 mM, 2.51 mM, and 0.149 mM respectively. Lac 1 activity was remarkably inhibited by the chloride ion, in a reversible manner. Lac 1 activity was also inhibited by thiol compounds. PMID:21389619

  20. [Role of the NO Synthase System in Response to Abiotic Stress Factors for Basidiomycetes Lentinula edodes and Grifola frondosa].

    PubMed

    Loshchinina, E A; Nikitina, V E

    2016-01-01

    Effect of stressors (unfavorable pH and temperature or carbon and nitrogen limitation) on the synthesis of the components of the NO synthase signaling system was studied in submerged cultures of xylotrophic basidiomycetes Lentinula edodes and Grifola frondosa. Marker compounds of the NO synthase signaling system were found in both cultures. A simultaneous increase of the concentrations of NO and citrulline in the culture liquid of the basidiomycetes grown at superoptimal pH and in nitrogen-limited medium indicates the activation of the NO synthase signaling system under such stress conditions. PMID:27476203

  1. A novel breeding strategy for new strains of Hypsizygus marmoreus and Grifola frondosa based on ligninolytic enzymes.

    PubMed

    Sun, Shujing; Li, Xiaojing; Ruan, Lingyun; Zhang, Liaoyuan; Hu, Kaihui

    2014-07-01

    A novel breeding strategy for new strains of Hypsizygus marmoreus and Grifola frondosa using ligninolytic enzymes as markers was evaluated with the detection and analysis of activities and composition of 15 edible fungi. The results showed that the activity and composition of ligninolytic enzyme system varied in response to changes of fungal strains. By analyzing the growth rate of mycelia and their ability to produce ligninolytic enzymes, H. marmoreus and P. geesteranus, G. frondosa and P. sajor-caju were screened for further study. Three colonies of 26 regenerated colonies of H. marmoreus and P. geesteranus protoplast fusion and one colony of 48 regenerated colonies of G. frondosa and P. sajor-caju were selected respectively. At the same time, these four strains were identified using RAPD and ISSR molecular markers. The results showed that the strains HM5G1 and PS7F1 are new strains and have low similarity to parental strains H. marmoreus and G. frondosa. These results are supported by the results of antagonism tests. These two fusants were significantly higher in their ligninolytic enzyme activity than H. marmoreus and G. frondosa. The growth rates of strains HM5G1and PS7F1 were also noticeably higher than those of H. marmoreus and G. frondosa, by 1.36 and 1.5 times respectively. The biological efficiency of the strain HM5G1 was 11.5% higher than that of the parental strain H. marmoreus. This work suggests that it is an efficient way of breeding new strains to use the decolorization of ligninolytic enzymes as a preliminary screening marker. PMID:24535613

  2. Effect of dietary Maitake (Grifola frondosa) mushrooms on plasma cholesterol and hepatic gene expression in cholesterol-fed mice.

    PubMed

    Sato, Mayumi; Tokuji, Yoshihiko; Yoneyama, Shozo; Fujii-Akiyama, Kyoko; Kinoshita, Mikio; Chiji, Hideyuki; Ohnishi, Masao

    2013-01-01

    To investigate the effects of dietary Grifola frondosa on cholesterol, normal mice were fed a diet containing 1% cholesterol (HC group) or 1% cholesterol and 10% freeze-dried G. frondosa powder (HC+G group) for 4 weeks and hepatic and plasma lipid levels were compared with those of a cholesterol-free diet-fed mice (N group). Hepatic total cholesterol (TC), triacylglycerol contents were considerably increased and plasma TC / phospholipid (PL) was also increased significantly in the HC group compared with the N group. However, plasma TC content decreased in the HC+G group compared with the HC group. To characterize the mechanisms responsible for lowered plasma cholesterol in G. frondosa-supplemented mice, hepatic gene expression was profiled using DNA microarray and gene ontology. Genome analyses revealed that de novo cholesterol synthesis genes were suppressed following cholesterol intake. However, expression of bile acid biosynthesis and low-density lipoprotein receptor genes showed little change. Scarb1, Abcg5, and Abcg8, involved in cholesterol transport and excretion, were slightly upregulated in the HC+G group compared with the HC group. These data indicate the plasma cholesterol-lowering effect of G. frondosa. Moreover, fatty acid (FA) β-oxidation was promoted via adipocytokine signaling pathways, and Saa, encodes serum amyloid A related to arteriosclerosis, was suppressed in the HC+G group. PMID:24292357

  3. Purification and characterization of a novel small-molecule polysaccharide from the Maitake medicinal mushroom Grifola frondosa (higher Basidiomycetes).

    PubMed

    Zhou, Changyan; Qiao, Yanru; Tang, Qingjiu; Jia, Wei; Liu, Yanfang; Wu, Aizhong

    2013-01-01

    A novel small-molecule polysaccharide with a molecular mass of 2.6 kDa, was isolated from the culinary-medicinal Maitake mushroom Grifola frondosa. GFPS is composed of fucose, rhamnose, galactose, glucose, and mannose; galactose, glucose, and mannose were the dominant monosaccharides. Absorption peaks at 1077 cm-1, 1024 cm-1, and 873 cm-1, as revealed by infrared spectrum, suggesting that GFPS consists of pyranoside. GFPS significantly enhanced the production of nitric oxide and secretion of cytokines (tumor necrosis factor-α, interleukin-1β, and interleukin-δ) from macrophages in vitro. These results indicate that this novel small-molecule polysaccharide might be beneficial for immune defense. PMID:23557366

  4. Functional polysaccharides from Grifola frondosa aqueous extract inhibit atopic dermatitis-like skin lesions in NC/Nga mice.

    PubMed

    Park, Hyeon Soo; Hwang, Yong Hyeon; Kim, Mun Ki; Hong, Gyeong Eun; Lee, Ho Jeong; Nagappan, Arulkumar; Yumnam, Silvia; Kim, Eun Hee; Heo, Jeong Doo; Lee, Sang Joon; Won, Chung Kil; Kim, Gon Sup

    2015-01-01

    Grifola frondosa (GF), distributed widely in far east Asia including Korea, is popularly used as traditional medicines and health supplementary foods, especially for enhancing the immune functions of the body. To extend the application of GF polysaccharides (GFP) for atopic dermatitis (AD), we investigated the effects of GFP on the 2,4-dinitrochlorobenzene-induced AD-like skin lesion in NC/Nga mice. GFP treatment significantly reduced the dorsa skin dermatitis score and combination treatment with GFP, and dexamethasone has a synergistic effect in AD-like skin lesion by reduced Serum IgE, mast cells infiltration, and cytokines expression. These results indicate that GFP suppressed the AD-like skin lesions by controlling the Th-1/Th-2-type cytokines in NC/Nga mice. These findings strongly suggest that GFP can be useful for AD patients as a novel therapeutic agent and might be used for corticosteroids replacement or supplement agent. PMID:25248662

  5. Control of Grifola frondosa Morphology by Agitation and Aeration for Improving Mycelia and Exo-Polymer Production.

    PubMed

    Cui, Feng-Jie; Chen, Xiao-Xiao; Liu, Wei-Min; Sun, Wen-Jing; Huo, Shuhao; Yang, Yan

    2016-06-01

    The present study describes the improved mycelia and exo-polymer production under control of Grifola frondosa morphology by changing the aeration rate and agitation intensity in a 25-L stirred fermentor. The aeration rate of 1.0 vvm yielded a highest mycelia biomass of 24.754 g/L with the lowest pellet percentage of 20.5 %. The maximum exo-polymer (2.324 g/L) was achieved at 0.75 vvm with mycelia polysaccharide production (0.321 g/g), whereas clumps and filaments dominated the ratios of 45.6 and 33.9 %, respectively. The change of aeration rate and agitation intensity had slight influence on the monosaccharide compositions in exo-polymers and significantly affected glucose and mannose contents in the mycelia polysaccharides. These findings will provide a clue for exploring the relationship between fermentation parameters, morphologies, and polysaccharide synthesis pathway of G. frondosa. PMID:26861733

  6. Structural analysis of anti-tumor heteropolysaccharide GFPS1b from the cultured mycelia of Grifola frondosa GF9801.

    PubMed

    Cui, F J; Tao, W Y; Xu, Z H; Guo, W J; Xu, H Y; Ao, Z H; Jin, J; Wei, Y Q

    2007-01-01

    A 21-kDa heteropolysaccharide, coded as GFPS1b, was obtained from the cultured mycelia of Grifola frondosa GF9801 by hot-water extraction, ethanol precipitation, and fractioned by DEAE Sepharose Fast-flow, followed by the purification with Sephadex G-100 column chromatography using an AKTA purifier. It exhibited more potent anti-proliferative activity on MCF-7 cells than other polysaccharide fractions. GFPS1b was an acidic polysaccharide with approximately 16.60% protein and 4.3% uronic acid. Gas chromatography of absolute acid hydrolysate of GFPS1b suggested that it was composed of D-glucose, D-galactose, and L-arabinose with a molar ratio of 4:2:1. Periodate oxidation, Smith degradation, partial acid hydrolyzation, methylation analysis, FT-IR, and (1)H, (13)C NMR spectroscopy analysis revealed that GFPS1b had a backbone consisting of alpha-(1-->4)-linked D-galacopyranosyl and alpha-(1-->3)-linked D-glucopyranosyl residues substituted at O-6 with glycosyl residues composed of alpha-L-arabinose-(1-->4)-alpha-D-glucose (1--> linked residues. PMID:16459075

  7. Purification and Partial Characterization of a Novel Anti-tumor Glycoprotein from Cultured Mycelia of Grifola frondosa.

    PubMed

    Cui, Fengjie; Zan, Xinyi; Li, Yunhong; Yang, Yan; Sun, Wenjing; Zhou, Qiang; Yu, Silian; Dong, Ying

    2013-10-25

    A novel glycoprotein GFG-3a with the molecular weight of 88.01 kDa and potent anti-tumor activity was isolated from the cultured mycelia of Grifola frondosa GF9801. GFG-3a was heat-sensitive with the decreasing anti-proliferative activity after treated from 56°C to 100°C for 10-120min. GFG-3a was a glycoprotein with O-glycosylation and contained 6.20% carbohydrate composed of D-arabinose, D-fructose, D-mannose, and D-glucose with a molar ratio of 1.33:4.51:2.46:1.00. FT-IR and NMR spectra proved that GFG-3a contained protein and carbohydrate portions with 3-O-methyl-galactose residues, (1→4)-linked β- galactose residues, and β-linked glucose residues. Circular dichroism (CD) revealed that GFG-3a was a predominantly β-sheet glycoprotein with a relatively small α-helical content. Protein sequencing and 3D model of GFG-3a were finally obtained by using MALDI-TOF-MS, NCBI blast search and online SWISS-MODLE Workspace service. Our findings will be a reference for the further structure-activity relationship analysis of the mushroom glycoproteins. PMID:24512992

  8. Anti-tumor and immunomodulatory activity of selenium (Se)-polysaccharide from Se-enriched Grifola frondosa.

    PubMed

    Mao, Guang-Hua; Ren, Yi; Li, Qian; Wu, Hui-Yu; Jin, Dun; Zhao, Ting; Xu, Cai-Quan; Zhang, Deng-Hong; Jia, Qing-Dong; Bai, Yan-Peng; Yang, Liu-Qing; Wu, Xiang-Yang

    2016-01-01

    A polysaccharide termed Se-GP11 was extracted and purified from Se-enriched Grifola frondosa in our previous study. This study investigated the characterization, anti-tumor and immunomodulatory activity of Se-GP11. The results showed that Se-GP11 was composed of mannose, glucose and galactose with a molar ratio of 1:4.91:2.41. The weight-average molecular weight (Mw) and weight-average mean square radius (Rw) of Se-GP11 in 0.1M sodium chloride solution were 3.3×10(4)Da and 32.8 nm. Se-GP11 existed as a globular conformation with random coil structure. Se-GP11 had no anti-tumor activity against HepG-2 cells in vitro, and it significantly inhibited the growth of Heps tumor in vivo. Se-GP11 increased the relatively thymus and spleen weights as well as serum necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) levels. In addition, Se-GP11 promoted the phagocytosis and NO production of RAW264.7 as compared with that of the normal control group. The results revealed that the Se-GP11 may exhibit the anti-tumor through improving immunologic function of the tumor bearing mice. PMID:26522247

  9. Inhibitory potential of Grifola frondosa bioactive fractions on α-amylase and α-glucosidase for management of hyperglycemia.

    PubMed

    Su, Chun-Han; Lu, Tzy-Ming; Lai, Min-Nan; Ng, Lean-Teik

    2013-01-01

    This study examined the inhibitory effects of Grifola frondosa (GF), a medicinal mushroom popularly consumed in traditional medicine and health food, on digestive enzymes related to type 2 diabetes; chemical profiles and inhibitory kinetics of its bioactive fractions were also analyzed. Results showed that all GF extracts showed weak anti-α-amylase activity; however, strong anti-α-glucosidase activity was noted on GF n-hexane extract (GF-H). Further fractionation confirmed that compared with acarbose (a commercial α-glucosidase inhibitor), the nonpolar fraction of GF possessed a stronger anti-α-glucosidase activity but a weaker anti-α-amylase activity. These activities were not derived from ergosterol and ergosterol peroxide, two major compounds of this fraction. The inhibitory kinetics of GF-H on α-glucosidase was competitive inhibition. GF-H was as good as acarbose in inhibiting the starch digestion in vitro. Oleic acid and linoleic acid could be the major active constituents that have contributed to the potency of GF in inhibiting α-glucosidase activity. PMID:24033596

  10. Grifola frondosa Glycoprotein GFG-3a Arrests S phase, Alters Proteome, and Induces Apoptosis in Human Gastric Cancer Cells.

    PubMed

    Cui, Fengjie; Zan, Xinyi; Li, Yunhong; Sun, Wenjing; Yang, Yan; Ping, Lifeng

    2016-01-01

    GFG-3a is a novel glycoprotein previously purified from the fermented mycelia of Grifola frondosa with novel sugar compositions and protein sequencing. The present study aims to investigate its effects on the cell cycle, differential proteins expression, and apoptosis of human gastric cancer SGC-7901 cells. Our findings revealed that GFG-3a induced the cell apoptosis and arrested cell cycle at S phase. GFG-3a treatment resulted in the differential expression of 21 proteins in SGC-7901 cells by upregulating 10 proteins including RBBP4 associated with cell cycle arrest and downregulating 11 proteins including RUVBL1, NPM, HSP90AB1, and GRP78 involved in apoptosis and stress response. qRT-PCR and Western blot analysis also suggested that GFG-3a could increase the expressions of Caspase-8/-3, p53, Bax, and Bad while decrease the expressions of Bcl2, Bcl-xl, PI3K, and Akt1. These results indicated that the stress response, p53-dependent mitochondrial-mediated, Caspase-8/-3-dependent, and PI3k/Akt pathways were involved in the GFG-3a-induced apoptosis process in SGC-7901 cells. These findings might provide a basis to prevent or treat human gastric cancer with GFG-3a and understand the tumor-inhibitory molecular mechanisms of mushroom glycoproteins. PMID:27040446

  11. Characterization of a novel maitake (Grifola frondosa) protein that activates natural killer and dendritic cells and enhances antitumor immunity in mice.

    PubMed

    Tsao, Yao-Wei; Kuan, Yen-Chou; Wang, Jia-Lin; Sheu, Fuu

    2013-10-16

    Grifola frondosa, also known as maitake, is a culinary mushroom with immune-enhancing and antitumor effects. Numerous studies have investigated the activity of maitake polysaccharide extracts, but studies of maitake proteins are scarce. In this study, we purified and characterized a new G. frondosa protein, GFP, from maitake fruiting bodies. GFP is a nonglucan heterodimeric 83 kDa protein that consists of two 41 kDa subunits. GFP induced interferon-γ secretion by murine splenocytes and natural killer cells and activated the maturation of bone marrow-derived dendritic cells (BMDCs) via a TLR4-dependent mechanism. GFP-treated BMDCs promoted a Th1 response and exhibited significant antitumor activity when transferred into tumor-bearing mice. In conclusion, we are the first to reveal the critical role of GFP in modulating the immune response and to link the immune-enhancing effects of maitake to its antitumor activities. PMID:24020458

  12. Pharmacological and pharmacokinetic studies with agaricoglycerides, extracted from Grifola frondosa, in animal models of pain and inflammation.

    PubMed

    Han, Chuncho; Cui, Bo

    2012-08-01

    The objective of the present study was to demonstrate the anti-inflammatory and antinociceptive properties of agaricoglycerides of the fermented mushroom of Grifola frondosa (AGF). The effects of AGF on interleukin-1β (IL-1β) levels, tumor necrosis factor-α (TNF-α) levels, nuclear factor kappa B (NF-κB) levels, intercellular adhesion molecule-1 (ICAM-1) levels, cyclooxygenase-2 (COX-2) levels, and inducible nitric oxide synthase (iNOS) levels were determined by ELISA. The antinociceptive effects of AGF were also analyzed in acetic acid-induced pain model and formalin-induced inflammatory pain model, respectively. At the same time, the pharmacokinetic assay of AGF was also made. AGF at the dose level of 500 mg/kg significantly inhibited LPS-induced upregulation of NF-κB activation and the production of IL-1β, TNF-α, iNOS, ICAM-1, and COX-2. Moreover, AGF at the dose level of 500 mg/kg suppressed the acetic acid-induced abdominal constrictions (p < 0.05) and the formalin-induced spontaneous nociceptive behaviors (p < 0.05) in rats. The total plasma concentrations of drug after oral administration of AGF at the dose level of 500 mg/kg led to an improvement in oral bioavailability. It accounts for the anti-inflammatory and antinociceptive activity of AGF. The present study demonstrated that AGF at the dose level of 500 mg/kg has important anti-inflammatory and antinociceptive effects in preclinical models of inflammation and in some models of pain and thus may be used as an alternative medicine for inflammatory pain. PMID:22327864

  13. Hypoglycemic effects of Grifola frondosa (Maitake) polysaccharides F2 and F3 through improvement of insulin resistance in diabetic rats.

    PubMed

    Xiao, Chun; Wu, Qingping; Xie, Yizhen; Zhang, Jumei; Tan, Jianbin

    2015-11-01

    Our laboratory has previously demonstrated that Grifola frondosa polysaccharides (GFPs) showed hypoglycemic effects. This study aimed to investigate which polysaccharide-enriched fractions of GFPs were the main active constituents, and to disclose their hypoglycemic mechanism. F2 and F3 were obtained from GFPs and their hypoglycemic effects were investigated. Fasting serum glucose (FSG) levels, fasting serum insulin (FSI) levels and a homeostasis model assessment of insulin resistance (HOMA-IR) were measured, and the hepatic mRNA levels of insulin receptor (IR), insulin receptor substrate-1 (IRS-1), protein tyrosine phosphatase-1B (PTP1B), phosphatidylinositol 3-kinase (PI3K) and Akt/protein kinase B (PKB) were determined by a quantitative polymerase chain reaction (qPCR). The activity of IR and IRS-1 were determined by an enzyme-linked immunosorbent assay (ELISA), and their phospho-protein levels were analyzed with western blotting. F2 and F3 significantly decreased the levels of FSG, FSI and HOMA-IR compared with a diabetic control group (P < 0.05). F2 and F3 increased the activity and mRNA levels of IR, and the latter also increased the mRNA levels of IRS-1. As for the protein levels of phospho-IR and IRS-1, both F2 and F3 increased the protein levels of IR (Try 1361), but decreased IRS-1 (Ser307). In the PI3K/Akt pathway, F3 increased the mRNA levels of PI3K and Akt, however, F2 inhibited PTP1B expression. F2 and F3 are presumed to cause an improvement in insulin resistance, triggered by the reactivation of IR and IRS-1. PMID:26311233

  14. Submerged-Culture Mycelia and Broth of the Maitake Medicinal Mushroom Grifola frondosa (Higher Basidiomycetes) Alleviate Type 2 Diabetes-Induced Alterations in Immunocytic Function.

    PubMed

    Chen, Ya-Hui; Lee, Chien-Hsing; Hsu, Tai-Hao; Lo, Hui-Chen

    2015-01-01

    Type 2 Diabetes mellitus (T2DM), a disease with impaired glucose, protein and lipid metabolism, low-grade chronic inflammation, and immune dysfunction, is a global public health crisis. We previously demonstrated that Grifola frondosa has bioactivities in improving glycemic responses in diabetic rats. Herein, we investigated the immunomodulatory effects of the submerged-culture mycelia and broth of G. frondosa on the peripheral blood cells (PBL) and splenocytes. Male Wistar rats were administered with saline (normal rats) or streptozotocin plus nicotinamide (T2DM rats) and were intragastrically administered with placebo, fermented mycelia, broth, or mycelia plus broth (1 g kg-1 day-1) for two weeks. In normal rats, ingestion of mycelia significantly decreased monocytes and ingestion of mycelia and broth significantly decreased the productions of interferon (IFN)-γ and interleukin (IL)-4 from the PBL and splenocytes. In T2DM rats, ingestion of mycelia, broth, and mycelia plus broth significantly alleviated the increases in 2 h postprandial blood glucose and the productions of IFN-γ from the T-leukocytes, IL-4, and IL-6 from the monocytes and IL-4 from the T-splenocytes, as well as significantly improved the productions of tumor-necrosis factor-α from the macrophages. In conclusion, submerged-culture mycelia and broth of G. frondosa may decrease cell-medicated immunity in normal rats and improve hyperglycemia and diabetes-induced alterations in cell-medicated and innate immunities in T2DM rats. PMID:26349512

  15. Profiling of hepatic gene expression of mice fed with edible japanese mushrooms by DNA microarray analysis: comparison among Pleurotus ostreatus, Grifola frondosa, and Hypsizigus marmoreus.

    PubMed

    Sato, Mayumi; Tokuji, Yoshihiko; Yoneyama, Shozo; Fujii-Akiyama, Kyoko; Kinoshita, Mikio; Ohnishi, Masao

    2011-10-12

    To compare and estimate the effects of dietary intake of three kinds of mushrooms (Pleurotus ostreatus, Grifola frondosa, and Hypsizigus marmoreus), mice were fed a diet containing 10-14% of each mushroom for 4 weeks. Triacylglycerol in the liver and plasma decreased and plasma cholesterol increased in the P. ostreatus-fed group compared with those in the control group. Cholesterol in the liver was lower in the G. frondosa-fed group than in the control group, but no changes were found in the H. marmoreus-fed group. DNA microarray analysis of the liver revealed differences of gene expression patterns among mushrooms. Ctp1a and Fabp families were upregulated in the P. ostreatus-fed group, which were considered to promote lipid transport and β-oxidation. In the G. frondosa-fed group, not only the gene involved in signal transduction of innate immunity via TLR3 and interferon but also virus resistance genes, such as Mx1, Rsad2, and Oas1, were upregulated. PMID:21910414

  16. Immunomodulating activities of cultivated maitake medicinal mushroom Grifola frondosa (Dicks.: Fr.) S.F. Gray (higher Basidiomycetes) on peripheral blood mononuclear cells.

    PubMed

    Svagelj, Mirjan; Berovic, Marin; Gregori, Andrej; Wraber, Branka; Simcic, Sasa; Boh, Bojana

    2012-01-01

    Grifola frondosa is a culinary-medicinal mushroom that contains several physiologically active compounds, of which polysaccharides, specifically β-glucans, are known to possess immunomodulating properties. Its extracts are studied for application as adjuncts for chemotherapy, and experiments in animal models support the use of this mushroom for cancer treatment. The effect of extracts obtained from mushrooms cultivated on different substrates and their capacity of inducing the secretion of cytokines from human peripheral blood mononuclear cells were studied. The activity of extracts at concentrations 12.5, 100, and 200 μg/mL on induction of TNF-α, IFN-γ, and IL-12 was screened. Two extracts from substrates fortified with olive oil press cakes showed appreciable activity and induced the secretion of TNF-α, IL-12, and INF-γ. The extracts differed from the others in the amount of sugar, protein, and β-glucans, which can explain their higher activity. Present results show that different substrates and different source materials can reasonably modify the bioactivity of cultivated G. frondosa. PMID:23510175

  17. Structural characterization and immunomodulatory activity of Grifola frondosa polysaccharide via toll-like receptor 4-mitogen-activated protein kinases-nuclear factor κB pathways.

    PubMed

    Ma, Xiaolei; Meng, Meng; Han, Lirong; Cheng, Dai; Cao, Xiaohong; Wang, Chunling

    2016-06-15

    We isolated a neutral polysaccharide from the fruiting body of a mushroom Grifola frondosa (GFP-A). The aim of this study was to characterize a neutral α-d-polysaccharide derived from G. frondosa and evaluate its immunomodulatory effect on toll-like receptor 4, mitogen-activated protein kinases and nuclear factor κB pathways of protein expression in macrophages. The structural features of GFP-A were characterized by physicochemical and instrumental analyses. Its molecular weight was found to be 8.48 × 10(2) kDa. The main chain of GFP-A consisted of (1 → 4)-linked and (1 → 6)-linked α-d-glucopyranosyl, and (1 → 3,6)-linked α-d-mannopyranosyl residues, which branched at C-3. The branches consisted of (1 → 6)-linked α-d-galactopyranosyl and t-l-rhamnopyranosyl residues. An in vitro immunomodulatory assay for pro-inflammatory cytokines (interleukin-1β, interleukin-2, tumor necrosis factor alpha, etc.) using the macrophage cell line, RAW 264.7, revealed that GFP-A exhibited significant immunomodulatory activity by stimulating the toll-like receptor 4, mitogen-activated protein kinases to nuclear factor κB/pathway. PMID:27220562

  18. Solid-state cultivation of Grifola frondosa (Dicks: Fr) S.F. Gray biomass and immunostimulatory effects of fungal intra- and extracellular beta-polysaccharides.

    PubMed

    Svagelj, Mirjan; Berovic, Marin; Boh, Bojana; Menard, Anja; Simcic, Sasa; Wraber, Branka

    2008-01-01

    Grifola frondosa strain GF3, was cultivated on solid-state substrate consisting of milled whole corn plant (Zea mays) and olive press cake supplemented with mineral additives and olive oil. Maintenance of the moisture content in the solid substrate is of crucial importance. Moistures higher than 70% promote growth of G. frondosa mycelium and polysaccharide production. Four fractions of pure extracellular beta-D-glucans with total mass 127.2mg and four fractions of intracellular polysaccharides with total mass 47.2mg were isolated. Polysaccharides were further separated by ion-exchange, gel and affinity chromatography. Isolated polysaccharide fractions from fungal mycelium proved to induce moderate amounts of TNF-alpha in PBMC cells in vitro. The extent of TNF-alpha induction was up to 322pgmL(-1) at a polysaccharide concentration of 200microgmL(-1) for the intracellular fraction. The TNF-alpha inducing activity is comparable to romurtide, which has been used as a supporting therapy in cancer patients treated with radiotherapy and/or chemotherapy. PMID:18786661

  19. MT-α-glucan from the fruit body of the maitake medicinal mushroom Grifola frondosa (higher Basidiomyetes) shows protective effects for hypoglycemic pancreatic β-cells.

    PubMed

    Lei, Hong; Zhang, Minmin; Wang, Qin; Guo, Shuzhen; Han, Juncheng; Sun, Hanju; Wu, Wutong

    2013-01-01

    The hypoglycemic effect of an α-glucan (designated here as MT-α-glucan) from the fruit body of the Maitake medicinal mushroom, Grifola frondosa, on a murine type 2 diabetes mellitus (T2DM) model was evaluated. Body weight and levels of fasting plasma glucose, glycosylated hemoglobin, triglycerides, cholesterol, free fatty acid, nitric oxide (NO), NO synthase, inducible NO synthase, and hepatic malondialdehyde content decreased significantly when MT-α-glucan was administered to T2DM mice. The content of serum insulin, hepatic glycogen, and reduced glutathione and the activity of superoxide dismutase and glutathione peroxidase increased significantly when MT-α-glucan was administered to T2DM mice. Histopathological changes of the pancreas were ameliorated in the treatment group. These data suggest that MT-α-glucan has a hypoglycemic effect on T2DM mice, which might be related to its protective effect of pancreatic β-cells exerted by decreasing levels of factors that destroy β-cells, such as oxidative stress and NO synthesis. PMID:23796219

  20. Investigation of the Optimum Farming Temperature for Grifola frondosa and Growth Promotion using the Bio-Electric Potential as an Index

    NASA Astrophysics Data System (ADS)

    Yanagibashi, Hideyuki; Hirama, Junji; Matsuda, Masato; Miyamoto, Toshio

    The purpose of this study was to investigate the optimum farming conditions for mushrooms from the view point of engineering field. As the bio-electric potential of mushrooms is considered to be closely related to the activation of mushroom cells, this relationship has been used to analyze the dependence of the morphogenetic characteristics of Grifola frondosa on farming temperatures (from 16 to 22 degree C). The experimental results indicated that a maximum response was exhibited, with correspondingly favorable morphogenesis obtained at 18 degree C. Based on the experimental results, including those in a previous study, it was assumed that the larger the bio-electric potential becomes, the higher the growth yield reaches. In order to support this assumption, growth promotion was conducted by intentionally activating the bio-electric potential within the mushrooms by stimulating them with short bursts of illumination. The resulting observation of growth promotion permitted the conclusion that the bio-electric potential can, indeed, be regarded as an index of growth.

  1. Oral administration of soluble β-glucans extracted from Grifola frondosa induces systemic antitumor immune response and decreases immunosuppression in tumor-bearing mice.

    PubMed

    Masuda, Yuki; Inoue, Hiroko; Ohta, Hiroya; Miyake, Ayumi; Konishi, Morichika; Nanba, Hiroaki

    2013-07-01

    Maitake D (MD)-Fraction is a highly purified soluble β-glucan derived from Grifola frondosa (an oriental edible mushroom). Intraperitoneal (i.p.) injection of MD-Fraction has been reported to inhibit tumor growth via enhancement of the host immune system. In this study, we demonstrated that oral administration of MD-Fraction as well as i.p. injection significantly inhibited tumor growth in murine tumor models. After oral administration, MD-Fraction was not transferred to the blood in its free form but was captured by antigen-presenting cells such as macrophages and dendritic cells (DCs) present in the Peyer's patch. The captured MD-Fraction was then transported to the spleen, thereby inducing the systemic immune response. Our study showed that MD-Fraction directly induced DC maturation via a C-type lectin receptor dectin-1 pathway. The therapeutic response of orally administered MD-Fraction was associated with (i) induced systemic tumor-antigen specific T cell response via dectin-1-dependent activation of DCs, (ii) increased infiltration of the activated T cells into the tumor and (iii) decreased number of tumor-caused immunosuppressive cells such as regulatory T cells and myeloid-derived suppressor cells. Our preclinical study suggests that MD-Fraction is a useful oral therapeutic agent in the management of patients with cancer. PMID:23280601

  2. A heteropolysaccharide, L-fuco-D-manno-1,6-α-D-galactan extracted from Grifola frondosa and antiangiogenic activity of its sulfated derivative.

    PubMed

    Wang, Ying; Shen, Xiaokun; Liao, Wenfeng; Fang, Jianping; Chen, Xia; Dong, Qun; Ding, Kan

    2014-01-30

    The tumor growth and metastasis are angiogenesis dependent, thus blockade of angiogenesis is a promising approach for treatment of cancer. Herein we reported the structural and biological features of a novel water-soluble polysaccharide named GFPW from the fruit body of Grifola frondosa. Chemical and spectral analysis revealed that GFPW with an average molecular weight of 15.7 kDa, possessed a backbone consisting of α-1,6-linked galactopyranosyl residues, with branches attached to O-2 of α-1,3-linked fucose residues and α-terminal mannose. By the chlorosulfonic acid-pyridine method, we prepared a sulfated derivative of GFPW, Sul-GFPW, with a substitution degree of 0.33. According to the (13)C NMR spectrum, the substitution position was deduced at C-2 and C-3. The angiogenesis assays in vitro showed that Sul-GFPW significantly inhibited endothelial cell proliferation in a dose- and time-dependent manner, and reduced endothelial cell migration and tube formation as well. PMID:24299820

  3. Cold-water extracts of Grifola frondosa and its purified active fraction inhibit hepatocellular carcinoma in vitro and in vivo.

    PubMed

    Lin, Chia-Hung; Chang, Ching-Yao; Lee, Kuan-Rong; Lin, Hui-Ju; Lin, Wu-Chou; Chen, Ter-Hsin; Wan, Lei

    2016-07-01

    Mushrooms are used in traditional Chinese medicine to treat a variety of diseases. Grifola frondosa (GF) is an edible mushroom indigenous to many Asian countries with a large fruiting body characterized by overlapping caps. In particular, GF is known for its anti-tumor activity, which has been targeted by scientific and clinical research. This study aimed to investigate the effects of the cold-water extract of GF (GFW) and its active fraction (GFW-GF) on autophagy and apoptosis, and the underlying mechanisms in vitro and in vivo Our results revealed that GFW and GFW-GF inhibited phosphatidylinositol 3-kinase (PI3K) and stimulated c-Jun N-terminal kinase (JNK) pathways, thereby inducing autophagy. We also demonstrated that GFW and GFW-GF inhibited proliferation, induced cell cycle arrest, and apoptosis in Hep3B hepatoma cells. GFW and GFW-GF markedly arrested cells in S phase and promoted cleavage of caspase-3 and -9. In addition, GFW and GFW-GF decreased the expression levels of the anti-apoptotic proteins protein kinase B and extracellular signal-regulated kinase. We also found that GFW significantly inhibited tumor growth in nude mice implanted with Hep3B cells. Our work demonstrates that GF and its active fraction inhibit hepatoma growth by inducing autophagy and apoptosis. PMID:27013543

  4. Inducement of cytokine release by GFPBW2, a novel polysaccharide from fruit bodies of Grifola frondosa , through dectin-1 in macrophages.

    PubMed

    Wang, Ying; Fang, Jianping; Ni, Xinyan; Li, Jie; Liu, Qin; Dong, Qun; Duan, Jinyou; Ding, Kan

    2013-11-27

    Polysaccharides, especially β-glucans isolated from various species of mushrooms, are considered as biological response modifiers (BRMs) to be widely used in the treatment of cancer, especially due to their immunostimulatory activity. We herein characterized the structure of a novel water-soluble homogeneous polysaccharide (GFPBW2) from the fruit bodies of mushroom Grifola frondosa and investigated its immunomodulatory activity in vitro. GFPBW2 was purified from the alkali-extracted fractions by stepwise elution with a molecular weight of 26.2 kDa. On the basis of infrared and NMR spectroscopy, methylation and monosaccharide composition analysis, partial acid hydrolysis, and Smith degradation, its structure was elucidated to possess a backbone consisting of β-d-1,3- and β-d-1,4-linked glucopyranosyl residues, with branches attached to O-6 of β-d-1,3-linked glucopyranosyl residues. Functionally, it is an effective inducer of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) secretion in murine resident peritoneal macrophages. Using quartz crystal microbalance (QCM) analysis, we found that GFPBW2 could bind dendritic cell-associated C-type lectin-1 (Dectin-1) with an affinity constant (Kd) value of 1.08 × 10 (-7) M, while it could activate Syk and enhance TNF-α production in RAW264.7 cells overexpressing wild type but not mutant Dectin-1. Furthermore, Syk, NF-κB signaling, and cytokine release in resident peritoneal macrophages induced by GFPBW2 could be significantly inhibited by a specific Dectin-1 blocking reagent, Laminarin. These data suggested that GFPBW2 might be a potential ligand of Dectin-1, and the potential of GFPBW2 to activate macrophage through triggering cytokine secretion might be attributed, at least in part, to the involvement of Dectin-1. PMID:24229406

  5. Soluble β-glucan from Grifola frondosa induces proliferation and Dectin-1/Syk signaling in resident macrophages via the GM-CSF autocrine pathway.

    PubMed

    Masuda, Yuki; Togo, Takuya; Mizuno, Shigeto; Konishi, Morichika; Nanba, Hiroaki

    2012-04-01

    MD-Fraction, a highly purified, soluble β-(1,3) (1,6)-glucan obtained from Grifola frondosa (an oriental edible mushroom), has been reported to inhibit tumor growth by modulating host immunity. β-Glucan, a major component of the fungal cell wall, is generally recognized by PRRs expressed on macrophages and DCs, such as Dectin-1, and the ability of β-glucans to modulate host immunity is influenced by their structure and purity. Most cellular studies have used particulate β-glucans, such as yeast zymosan (crude β-glucan) and curdlan (purified β-glucan). However, little is known about the cellular mechanism of soluble β-glucans, including MD-Fraction, despite significant therapeutic implications. In this study, we investigated the cellular mechanism of MD-Fraction in murine resident macrophages and compared it with two well-known β-glucan particles. MD-Fraction induced GM-CSF production rapidly through Dectin-1-independent ERK and p38 MAPK activation. Subsequently, MD-Fraction-induced GM-CSF enhanced proliferation and Dectin-1 expression, which permitted Dectin-1-mediated TNF-α induction through the Syk pathway. Curdlan induced not only the proliferation and activation of Dectin-1/Syk signaling in a manner similar to MD-Fraction but also the uncontrolled, proinflammatory cytokine response. Contrastingly, zymosan reduced proliferation and Dectin-1 expression significantly, indicating that the mechanism of macrophage activation by MD-Fraction differs from that of zymosan. This is the first study to demonstrate that purified β-glucans, such as MD-Fraction and curdlan, induce GM-CSF production directly, resulting in Dectin-1/Syk activation in resident macrophages. In conclusion, we demonstrated that MD-Fraction induces cell proliferation and cytokine production without excessive inflammation in resident macrophages, supporting its immunotherapeutic potential. PMID:22028332

  6. Structure of a β-glucan from Grifola frondosa and its antitumor effect by activating Dectin-1/Syk/NF-κB signaling.

    PubMed

    Fang, Jianping; Wang, Ying; Lv, Xiaofen; Shen, Xiaokun; Ni, Xinyan; Ding, Kan

    2012-08-01

    A soluble homogeneous β-glucan, GFPBW1, with a molecular mass of 300 kDa was purified from the fraction of the fruit bodies of Grifola frondosa extracted with 5% NaOH. Using various methods, such as infrared spectroscopy, NMR, methylation and monosaccharide composition analysis, its structure was determined to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue. It induced TNF-α and IL-6 production and the activation of Syk and NF-κB signaling in resident peritoneal macrophages from ICR mice, which could be significantly inhibited by the blocking reagent laminarin. A competitive phagocytosis assay with FITC-zymosan indicated that GFPBW1 could bind to DC-associated C-type lectin 1 (Dectin-1). The TNF-α secretion and activation of Syk/NF-κB signaling triggered by GFPBW1 were enhanced in RAW264.7 cells overexpressing wild but not mutant (Δ38 and Y15S) Dectin-1. Furthermore, GFPBW1 potentiated the Concanavalin A-induced proliferative response of splenocytes and inhibited Sarcoma-180 growth allografted in ICR mice but not in immunodeficient BALB/c nu/nu mice. These results suggested that the antitumor activity of GFPBW1 was partially associated with the activation of macrophages via the Dectin-1/Syk/NF-κB signaling pathway. This molecule could be a promising biological response modifier with clear application for antitumor therapies. PMID:22744837

  7. Soluble β-glucan from Grifola frondosa induces tumor regression in synergy with TLR9 agonist via dendritic cell-mediated immunity.

    PubMed

    Masuda, Yuki; Nawa, Daiki; Nakayama, Yoshiaki; Konishi, Morichika; Nanba, Hiroaki

    2015-12-01

    The maturation of dendritic cells into more-immunostimulatory dendritic cells by stimulation with different combinations of immunologic agents is expected to provide efficient, adoptive immunotherapy against cancer. Soluble β-glucan maitake D-fraction, extracted from the maitake mushroom Grifola frondosa, acts as a potent immunotherapeutic agent, eliciting innate and adoptive immune responses, thereby contributing to its antitumor activity. Here, we evaluated the efficacy of maitake D-fraction, in combination with a Toll-like receptor agonist, to treat tumors in a murine model. Our results showed that maitake D-fraction, in combination with the Toll-like receptor 9 agonist, cytosine-phosphate-guanine oligodeoxynucleotide, synergistically increased the expression of dendritic cell maturation markers and interleukin-12 production in dendritic cells, but it did not increase interleukin-10 production, generating strong effector dendritic cells with an augmented capacity for efficiently priming an antigen-specific, T helper 1-type T cell response. Maitake D-fraction enhances cytosine-phosphate-guanine oligodeoxynucleotide-induced dendritic cell maturation and cytokine responses in a dectin-1-dependent pathway. We further showed that a combination therapy using cytosine-phosphate-guanine oligodeoxynucleotide and maitake D-fraction was highly effective, either as adjuvants for dendritic cell vaccination or by direct administration against murine tumor. Therapeutic responses to direct administration were associated with increased CD11c(+) dendritic cells in the tumor site and the induction of interferon-γ-producing CD4(+) and CD8(+) T cells. Our results indicate that maitake D-fraction and cytosine-phosphate-guanine oligodeoxynucleotide synergistically activated dendritic cells, resulting in tumor regression via an antitumor T helper cell 1-type response. Our findings provide the basis for a potent antitumor therapy using a novel combination of immunologic agents for

  8. A phase I/II trial of a polysaccharide extract from Grifola frondosa (Maitake mushroom) in breast cancer patients: immunological effects

    PubMed Central

    Lin, Hong; Seidman, Andrew; Fornier, Monica; D’Andrea, Gabriella; Wesa, Kathleen; Yeung, Simon; Cunningham-Rundles, Susanna; Vickers, Andrew J.; Cassileth, Barrie

    2013-01-01

    Background Cancer patients commonly use dietary supplements to “boost immune function”. A polysaccharide extract from Grifola frondosa (Maitake extract) showed immunomodulatory effects in preclinical studies and therefore the potential for clinical use. Whether oral administration in human produces measurable immunologic effects, however, is unknown. Methods In a phase I/II dose escalation trial, 34 postmenopausal breast cancer patients, free of disease after initial treatment, were enrolled sequentially in five cohorts. Maitake liquid extract was taken orally at 0.1, 0.5, 1.5, 3, or 5 mg/kg twice daily for 3 weeks. Peripheral blood was collected at days −7, 0 (prior to the first dosing), 7, 14, and 21 for ex vivo analyses. The primary endpoints were safety and tolerability. Results No dose-limiting toxicity was encountered. Two patients withdrew prior to completion of the study due to grade I possibly related side effects: nausea and joint swelling in one patient; rash and pruritus in the second. There was a statistically significant association between Maitake and immunologic function (p < 0.0005). Increasing doses of Maitake increased some immunologic parameters and depressed others; the dose–response curves for many endpoints were non-monotonic with intermediate doses having either immune enhancing or immune suppressant effects compared with both high and low doses. Conclusions Oral administration of a polysaccharide extract from Maitake mushroom is associated with both immunologically stimulatory and inhibitory measurable effects in peripheral blood. Cancer patients should be made aware of the fact that botanical agents produce more complex effects than assumed, and may depress as well as enhance immune function. PMID:19253021

  9. Effects of Lentinus edodes, Grifola frondosa and Pleurotus ostreatus administration on cancer outbreak, and activities of macrophages and lymphocytes in mice treated with a carcinogen, N-butyl-N-butanolnitrosoamine.

    PubMed

    Kurashige, S; Akuzawa, Y; Endo, F

    1997-05-01

    ICR mice were treated with a carcinogen, N-butyl-N'-butanolnitrosoamine BBN), every day for 8 consecutive weeks and the effects of oral administration of edible mushrooms on the induction of urinary bladder carcinoma and on the activities of macrophages and lymphocytes were studied. Bladder carcinoma were found in all 10 mice (100%) treated with BBN alone, while we observed carcinoma only in 9 of 17 mice (52.9%), in 7 of 15 mice (46.7%) and 13 of 20 mice (65.0%) treated with Lentinus edodes, Grifola frondosa and Pleurotus ostreatus, respectively. Chemotactic activity of macrophages was suppressed in mice treated with BBN alone but maintained almost the normal level in mice treated with BBN plus Lentinus, Grifola or Pleurotus. Lymphocytes collected from mice treated with BBN plus each mushroom showed almost normal blastogenic response against concanavalin A, although those from mice treated with BBN alone completely retarded their response. Cytotoxic activity of lymphocytes against Yac-1 cells was also maintained at a normal level in mice treated with BBN plus each mushroom. Whereas in mice treated with BBN alone significant depression of NK cell activity occurred. Significantly higher cytotoxic activity against P-815 cells was observed in lymphocytes from mice treated with BBN plus each mushroom than that in lymphocytes from normal mice or mice treated with BBN alone. PMID:9130004

  10. In Vitro Studies of Secondary Metabolite-Related Responses in Some Species of Genus Grifola (Agaricomycetes) from Argentina.

    PubMed

    Postemsky, Pablo Daniel; Curvetto, Nestor Raul

    2016-01-01

    Grifola gargal Singer and Grifola sordulenta (Mont.) Singer mushrooms are related to Grifola frondosa (Dicks.) Gray, which is well known for its medicinal properties. In vitro studies were performed to find a useful guide for optimizing the environmental parameters through biotransformation of lignocellulosic materials and basidiome development, also considering secondary metabolism-related responses (SMRRs) associated with these processes and the variability among species and strains; this optimization is necessary to make the mushroom's industrial cultivation profitable. Morphological features of mycelial cultures revealed that intraspecific variability was of taxonomic relevance. A low ligninolytic capacity in studied Grifola species was observed when compared with 2 control species: G. frondosa and Ganoderma lucidum. Experiments with nutrient media containing different carbohydrate sources indicated that G. gargal mycelia grew better in xylulose and G. sordulenta, in xylulose or cellulose; in addition, the latter species presented cellobiose dehydrogenase activity. An additional study of SMRRs under different light conditions (aroma, pigmentation, and morphogenic manifestations) showed that white light was better than blue, green, or red-filtered light at inducing advanced SMRRs. The results of SMRR stimulation are proposed as useful guidance for optimizing the environmental parameters for bioprocesses aimed at metabolite production. PMID:27481302

  11. Enhancement of wheat grain antioxidant activity by solid state fermentation with Grifola spp.

    PubMed

    Postemsky, Pablo; Curvetto, Néstor

    2014-05-01

    Grifola frondosa, Grifola gargal, and Grifola sordulenta are edible and medicinal mushrooms with antioxidant properties. To obtain wheat flour (Wf ) with a higher antioxidant activity than the one exhibited by regular Wf, solid state fermentation (SSF) of wheat grains with mycelia of those Grifola spp. was used to obtain biotransformed wheat grain (BWG) flour. The methanolic extract of control Wf and BWG flour of G. gargal, G. sordulenta, and G. frondosa (GfWG, GgWG, and GsWG, respectively) were studied for their radical scavenging (RS) activity against 2,2-diphenyl-1-picrylhydracyl (DPPH) and their Fe(III) reducing power (RP). The values for RS-EC50 decreased in BWG flour, therefore presenting a higher antioxidant activity: GgWG (0.56 mg/mL), GfWG (0.81 mg/mL), and GsWG (5.80 mg/mL) in comparison to Wf (57.60 mg/mL). The antioxidant content for this RS activity in terms of ascorbic acid content (RS-EQAA) was highest in GfWG, followed by GgWG and GsWG (71.73, 14.46, and 3.02 mg/g, respectively) and lowest in Wf (0.25 mg/g). The RP-EC50 values in GgWG, GfWG, and GsWG were low (0.55, 0.64, and 4.20 mg/mL, respectively) with respect to Wf (55.00 mg/mL). Compared with Wf (0.56 mg/g), the RP capacity in terms of ascorbic acid content (RP-EQAA) was very high in GfWG (193.67 mg/g) followed by GgWG and GsWG (31.42 and 8.74 mg/g, respectively). The high content in gallic acid equivalents was consistent with RS-EQ(AA) and RP-EQ(AA) contents. TLC revealed that antioxidant activity in BWG could be related to the presence of phenolic compounds. Thus, a valuable food alternative can easily be obtained with wheat grains, that is, by markedly increasing their antioxidant value through SSF with Grifola spp. PMID:24552201

  12. Submerged Culture of Grifola gargal and G. sordulenta (Higher Basidiomycetes) from Argentina as a Source of Mycelia with Antioxidant Activity.

    PubMed

    Postemsky, Pablo Daniel; Curvetto, Néstor Raúl

    2015-01-01

    Submerged culture is an alternative mycelium source for Grifola gargal and G. sordulenta, two rare edible mushrooms related to Grifola frondosa. This work studies their mycelia as a source of antioxidants. The efficient concentrations of methanolic extracts in both radical scavenging (RS) and reducing power (RP) abilities in G. gargal and in G. sordulenta showed a high antioxidant activity. In the experimental design used, the antioxidant activity mainly depended on the culture conditions rather than on the media composition. Irrespective of the basal culture medium, mycelium methanolic extracts of G. sordulenta obtained from culture in Erlenmeyer flasks showed equivalents to ascorbic acid (EQ(AA)) RS-EQ(AA) and RP-EQ(AA) contents higher than the corresponding values obtained with jar cultures. Under stationary cultivation, G. sordulenta produced approximately 50% higher content in both RS-EQ(AA) and RP-EQ(AA) than the medicinal mushroom G. frondosa. Phenolics correlated with RS-EQ(AA) and RP-EQ(AA) in G. gargal and with RP-EQ(AA) in G. sordulenta; besides, thin-layer chromatography showed these compounds to be at least in part related to the RS capacity. It is concluded that G. gargal and G. sordulenta mycelia are excellent sources of antioxidant metabolites. PMID:25746407

  13. Immune-enhancing effects of Maitake (Grifola frondosa) and Shiitake (Lentinula edodes) extracts

    PubMed Central

    Vetvickova, Jana

    2014-01-01

    Background The role of glucan in stimulation of immune reactions has been studied for several decades. In this report, we focused on the effects of orally administered glucan Maitake and Shiitake on immune reactions. Materials and methods We measured phagocytosis, NK cell activity, and secretion of IL-6, IL-12, IFN-γ as well as C-reactive protein (CRP) after 14 days of oral application of tested glucans. For comparison, active hexose correlated compound (AHCC) was used in all reactions. Results We found significant stimulation of defense reaction. In all cases, the most active was the Maitake-Shiitake combination, with Maitake alone being the second strongest, followed by Shiitake on its own and AHCC. Conclusions Short-term oral application of natural immunomodulating glucans from Maitake and Shiitake mushrooms strongly stimulated both the cellular and humoral branch of immune reactions. These activities were significantly higher than those of AHCC. PMID:25332990

  14. Effect of Maitake (Grifola frondosa) D-Fraction on the activation of NK cells in cancer patients.

    PubMed

    Kodama, Noriko; Komuta, Kiyoshi; Nanba, Hiroaki

    2003-01-01

    Maitake D-Fraction, extracted from maitake mushroom, has been reported to exert its antitumor effect in tumor-bearing mice by enhancing the immune system through activation of macrophages, T cells, and natural killer (NK) cells. In a previous study, the combination of immunotherapy with the maitake D-Fraction and chemotherapy suggested that the D-Fraction may have the potential to decrease the size of lung, liver, and breast tumors in cancer patients. In the present study, we administered maitake D-Fraction to cancer patients without anticancer drugs, and at the same time NK cell activity was monitored to investigate whether the activity is closely related with disease progression. The numbers of CD4(+) and CD8(+) cells in the peripheral blood were measured in 10 patients, and NK cell activity was assessed using K-562 cells as target cells. Serum soluble interleukin-2 receptor (sIL-2R) levels in three patients and the expression of tumor markers in four patients were determined by enzyme-linked immunosorbent assay. The slight changes observed in the CD4(+) and CD8(+) cell numbers were independent of disease severity or stage as well as serum sIL-2R levels. In contrast, maitake D-Fraction hindered metastatic progress, lessened the expression of tumor markers, and increased NK cell activity in all patients examined. Thus maitake D-Fraction appears to repress cancer progression and primarily exerts its effect through stimulation of NK activity. In addition, we conclude that measurement of NK cell activity may be a useful clinical parameter in monitoring disease progression during and following immunotherapy with maitake D-Fraction. PMID:14977447

  15. Anti-Inflammatory Constituents from Bidens frondosa.

    PubMed

    Le, Jiamei; Lu, Wenquan; Xiong, Xiaojuan; Wu, Zhijun; Chen, Wansheng

    2015-01-01

    A new polyacetylene glucoside (3E,5E,11E)-tridecatriene-7,9-diyne-1,2,13-triol-2-O-β-D-glucopyranoside (1), a new phenylpropanoid glucoside 2'-butoxyethylconiferin (2), and a new flavonoid glycoside 8,3',4'-trihydroxyflavone-7-O-(6''-O-p-coumaroyl)-β-D-glucopyranoside (3), have been isolated from Bidens frondosa together with fifty-three known compounds 4-56. The structures of these compounds were established by spectroscopic methods. mainly ESIMS, 1D- and 2D-NMR spectroscopic data. and comparison with literature data. Compounds 1-34, 36, 39, 43, 47, 51, and 52 were tested for inhibition of nuclear factor kappa B (NF-κB) in 293-NF-κB-luciferase report cell line induced by lipopolysaccharide (LPS), and compounds 1, 2, 3, 9, 15, 21, 24 and 51 were tested for the production of TNF-α, IL-1β, IL-6, IL-10 in RAW 264.7 macrophages induced by LPS. In conclusion, the isolated compounds 1, 2, 3, 9, 15, 21, 24 and 51 exhibited significant activity in anti-inflammatory activity assays. PMID:26473814

  16. A chemically sulfated polysaccharide from Grifola frondos induces HepG2 cell apoptosis by notch1-NF-κB pathway.

    PubMed

    Wang, Chun-ling; Meng, Meng; Liu, Sheng-bin; Wang, Li-rui; Hou, Li-hua; Cao, Xiao-hong

    2013-06-01

    Sulfated polysaccharides have been known to inhibit proliferation in tumor cells. However, the molecular mechanisms involved in sulfated polysaccharides-induced apoptosis are still uncharacterized. In this study, the effect of a chemically sulfated polysaccharide obtained from Grifola frondosa (S-GFB) on HepG2 cell proliferation and apoptosis-related mechanism were investigated. It was found that S-GFB inhibited proliferation of HepG2 cells in a dose-dependent manner with IC50 at 48 h of 61 μg ml(-1). The results of scanning electron micrographs indicated that S-GFB induced typical apoptotic morphological feature in HepG2 cells. Flow cytometric analysis demonstrated that S-GFB caused apoptosis of HepG2 cells through cells arrested at S phase. Western-blotting results showed that S-GFB inhibited notch1 expression, IκB-α degradation and NF-κB/p65 translocation from cytoplasm into nucleus. Simultaneously, the apoptotic mechanism of HepG2 cells induced by S-GFB was associated with down regulation of FLIP, and activation of caspase-3 and caspase-8. Taken together, these findings suggest that the S-GFB induces apoptosis through a notch1/NF-κB/p65-mediated caspase pathway. PMID:23618270

  17. Amelioration of Atherosclerosis by the New Medicinal Mushroom Grifola gargal Singer.

    PubMed

    Harada, Etsuko; D'Alessandro-Gabazza, Corina N; Toda, Masaaki; Morizono, Toshihiro; Chelakkot-Govindalayathil, Ayshwarya-Lakshmi; Roeen, Ziaurahman; Urawa, Masahito; Yasuma, Taro; Yano, Yutaka; Sumiya, Toshimitsu; Gabazza, Esteban C

    2015-08-01

    The beneficial effects of edible mushrooms for improving chronic intractable diseases have been documented. However, the antiatherogenic activity of the new medicinal mushroom Grifola gargal is unknown. Therefore, we evaluated whether Grifola gargal can prevent or delay the progression of atherosclerosis. Atherosclerosis was induced in ApoE lipoprotein-deficient mice by subcutaneous infusion of angiotensin II. Grifola gargal extract (GGE) was prepared and intraperitoneally injected. The weight of heart and vessels, dilatation/atheroma formation of thoracic and abdominal aorta, the percentage of peripheral granulocytes, and the blood concentration of MCP-1/CCL2 were significantly reduced in mice treated with GGE compared to untreated mice. By contrast, the percentage of regulatory T cells and the plasma concentration of SDF-1/CXCL12 were significantly increased in mice treated with the mushroom extract compared to untreated mice. In vitro, GGE significantly increased the secretion of SDF-1/CXCL12, VEGF, and TGF-β1 from fibroblasts compared to control. This study demonstrated for the first time that Grifola gargal therapy can enhance regulatory T cells and ameliorate atherosclerosis in mice. PMID:25799023

  18. Amelioration of Atherosclerosis by the New Medicinal Mushroom Grifola gargal Singer

    PubMed Central

    Harada, Etsuko; D'Alessandro-Gabazza, Corina N.; Toda, Masaaki; Morizono, Toshihiro; Chelakkot-Govindalayathil, Ayshwarya-Lakshmi; Roeen, Ziaurahman; Urawa, Masahito; Yasuma, Taro; Yano, Yutaka; Sumiya, Toshimitsu

    2015-01-01

    ABSTRACT The beneficial effects of edible mushrooms for improving chronic intractable diseases have been documented. However, the antiatherogenic activity of the new medicinal mushroom Grifola gargal is unknown. Therefore, we evaluated whether Grifola gargal can prevent or delay the progression of atherosclerosis. Atherosclerosis was induced in ApoE lipoprotein-deficient mice by subcutaneous infusion of angiotensin II. Grifola gargal extract (GGE) was prepared and intraperitoneally injected. The weight of heart and vessels, dilatation/atheroma formation of thoracic and abdominal aorta, the percentage of peripheral granulocytes, and the blood concentration of MCP-1/CCL2 were significantly reduced in mice treated with GGE compared to untreated mice. By contrast, the percentage of regulatory T cells and the plasma concentration of SDF-1/CXCL12 were significantly increased in mice treated with the mushroom extract compared to untreated mice. In vitro, GGE significantly increased the secretion of SDF-1/CXCL12, VEGF, and TGF-β1 from fibroblasts compared to control. This study demonstrated for the first time that Grifola gargal therapy can enhance regulatory T cells and ameliorate atherosclerosis in mice. PMID:25799023

  19. Role of Butea Frondosa in Ameliorating Gastric Markers in Induced Gastric Lesions of Rats

    PubMed Central

    Banji, D.; Banji, Otilia J. F.; Singh, Meenu; Annamalai, A. R.

    2011-01-01

    The study evaluated the ability of the alcohol extract of Butea frondosa to protect the gastro-duodenal lining from injury inflicted by acetic acid and pyloric ligation in rats. The induced gastric lesions lead to the generation of alkaline phosphatase and pepsin, which serve as important markers of gastric damage. Alcohol extract of Butea frondosa was administered in doses of 10, 100, 300 and 500 mg/kg as a single schedule and for the time dependent studies in a dose of 100 mg/kg for 7, 14, 21 and 28 days, respectively. Our studies reveal a decline in the formation of alkaline phosphatase and pepsin with 300 and 500 mg/kg of the extract and following treatment for 21 and 28 days, respectively. Extract of Butea frondosa produces significant diminution in the formation of gastric markers implying possible gastro-protective action. PMID:22457555

  20. Antibacterial activity in Strongylocentrotus droebachiensis (Echinoidea), Cucumaria frondosa (Holothuroidea), and Asterias rubens (Asteroidea).

    PubMed

    Haug, Tor; Kjuul, Anita K; Styrvold, Olaf B; Sandsdalen, Erling; Olsen, Ørjan M; Stensvåg, Klara

    2002-10-01

    A search for antibacterial activity in different body parts of the green sea urchin Strongylocentrotus droebachiensis, the common starfish Asterias rubens, and the sea cucumber Cucumaria frondosa was conducted. Antibacterial activity was detected in extracts from several tissues in all species tested, but mainly in the coelomocyte and body wall extracts. Relatively high antibacterial activity could also be detected in gastrointestinal organs and eggs from A. rubens and in eggs from C. frondosa. Differences between active extracts regarding hydrophobicity and sensitivity to heat and proteinase K treatment indicated that several different compounds were responsible for the antibacterial activities detected. Lysozyme-like activity could be detected in several tissues from A. rubens. Haemolytic activity could be detected in all species tested, especially in the body wall extracts. Results from the current study suggest that marine echinoderms are a potential source for the discovery of novel antibiotics. PMID:12445793

  1. Biological and physicochemical properties of two polysaccharides from the mycelia of Grifola umbellate.

    PubMed

    Bi, Yunpeng; Miao, Ye; Han, Yan; Xu, Jian; Wang, Qing

    2013-06-20

    In the present study, we firstly reported the antioxidant, antitumor and immunomodulatory effects of two polysaccharides (GUMP-1-1 and GUMP-1-2) isolated from Grifola umbellata mycelia. Chemical analysis indicated that two polysaccharide fractions contained different content of neutral sugar, uronic acid and protein, as well as varying monosaccharide compositions and average molecular weight. We found that they could significantly inhibit the growth of H22 implanted tumor and enhance the spleen index and splenocyte proliferation of H22 tumor-bearing mice. In addition, GUMP-1-2 had the stronger free radicals scavenging and ferrous ion chelating abilities than GUMP-1-1 in vitro. These results indicated that antitumor activity of two purified polysaccharides might be achieved by improving immune response and the different chemical composition and average molecular weight could affect their antitumor, antioxidant and immunomodulatory activities. PMID:23648036

  2. Allelopathy of the invasive plant Bidens frondosa on the seed germination of Geum japonicum var. chinense.

    PubMed

    Wang, X F; Hassani, D; Cheng, Z W; Wang, C Y; Wu, J

    2014-01-01

    Five gradient concentrations (0.02, 0.04, 0.06, 0.08, and 0.10 g/mL) of leaching liquors from the roots, stems, and leaves of the invasive plant Bidens frondosa were used as conditioning fluid to examine its influence on seed germination conditions of the native plant Geum japonicum var. chinense in Huangshan. All leaching liquors of organs suppressed the seed germination of Geum japonicum var. chinense and reduced the final germination percentage and rate, and increased the germination inhibition rate, with a bimodal dependence on concentration. The leaching liquor inhibited the seed germination significantly at the concentration of 0.02 g/mL respectively. The seed germination was also inhibited as the concentration reached to 0.04 g/mL and beyond. Hence the allelopathic effects of the organs were significantly enhanced respectively. This phenomenon represented the presence of allelopathy substances in the root, stem and leaf of Bidens frondosa. PMID:25511044

  3. NASA Solve

    NASA Video Gallery

    NASA Solve lists opportunities available to the general public to contribute to solving tough problems related to NASA’s mission through challenges, prize competitions, and crowdsourcing activities...

  4. Purification, characterization and cloning of tensilin, the collagen-fibril binding and tissue-stiffening factor from Cucumaria frondosa dermis.

    PubMed

    Tipper, Jennifer P; Lyons-Levy, Gillian; Atkinson, Mark A L; Trotter, John A

    2002-12-01

    The inner dermis of the sea cucumber, Cucumaria frondosa, is a mutable collagenous tissue characterized by rapid and reversible changes in its mechanical properties regulated by one or more protein effectors that are released from neurosecretory cells. One such effector, tensilin, is a collagen-fibril binding protein, named for its ability to induce dermis stiffening. Tensilin was purified using an affinity column constructed from C. frondosa collagen-fibrils. The protein migrates as a single band on SDS-PAGE (Mr approximately 33 kDa) and has an isoelectric point of 5.8. Equilibrium sedimentation experiments suggest a molecular mass of approximately 28.5-29.4 kDa. Carbohydrate analysis of tensilin revealed no measurable sugar content. The molar amount of tensilin was determined to be 0.38% that of collagen and 47% that of stiparin, a constitutive matrix glycoprotein. A full-length cDNA clone for tensilin was obtained from a C. frondosa inner dermis cDNA expression library. Predicted properties derived from the deduced peptide sequence were in agreement with those of the native protein. A noted feature of tensilin's deduced peptide sequence, particularly in its N-terminal domain, is its homology to tissue inhibitor of metalloproteinases. Tensilin's C-terminal tail has no known homology to other proteins but contains a putative collagen-fibril binding site. PMID:12524049

  5. Compositional characteristics and antioxidant properties of fresh and processed sea cucumber (Cucumaria frondosa).

    PubMed

    Zhong, Ying; Khan, Muhammad Ahmad; Shahidi, Fereidoon

    2007-02-21

    The antioxidant activity of fresh and rehydrated sea cucumber (Cucumaria frondosa) samples with/without internal organs was evaluated for the first time. In addition, their proximate, amino acid, and fatty acid compositions were examined. Rehydrated sea cucumber samples in distilled water were prepared from oven-dried products. All samples contained 83-90% moisture, but showed a significant difference among groups in their protein and lipid contents. Glutamic acid was the predominant amino acid in sea cucumber, followed by glycine and aspartic acid. Essential amino acids such as leucine and lysine were also present at high levels. The trend for free amino acid was different from that of total amino acids and varied among groups. Lipids in sea cucumber were dominated by eicosapentaenoic acid (EPA, C20:5n-3), ranging from 43.2 to 56.7% of the total fatty acids. Docosahexaenoic acid (DHA, C22:6n-3) was present at a much lower concentration of 2.0-5.8%. All sea cucumber samples exhibited radical scavenging property against 2,2'-azobis(2-aminopropane) dihydrochloride (AAPH) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, with rehydrated samples, especially those with internal organs, possessing higher antioxidant activity than their fresh counterparts. No correlation existed between radical scavenging capacity and total phenolics content, suggesting that other components, in addition to phenolic compounds, contribute to the antioxidant activity of sea cucumber. PMID:17243707

  6. Solving Quartics

    ERIC Educational Resources Information Center

    Kulkarni, R. G.

    2007-01-01

    A technique is presented, which is different from the well-known Ferrari's method, to solve a general quartic equation. Formulae for the four roots of quartic are derived. A numerical example verifies the formulae obtained.

  7. Solving Triangles

    ERIC Educational Resources Information Center

    Jarrrett, Joscelyn

    2005-01-01

    This article discusses the four categories of triangles that are standard in most textbooks when "solving" triangles: (a) Given the lengths of two sides and the measure of an angle opposite one of the two given sides, (b) Given the lengths of two sides and the measure of the included angle, (c) Given the lengths of all three sides, d) Given the…

  8. Novel Use of PIT Tags in Sea Cucumbers: Promising Results with the Commercial Species Cucumaria frondosa.

    PubMed

    Gianasi, Bruno L; Verkaik, Katie; Hamel, Jean-François; Mercier, Annie

    2015-01-01

    The lack of a reliable and innocuous mark-recapture method has limited studies that would provide essential information for the management of commercial sea cucumbers. Tagging sea cucumbers is notoriously difficult because of their plastic nature and autolysis capacities. The markers that have so far been tested, mainly on or through the body wall, were either lost rapidly or had major drawbacks (e.g. suitable only for batch identification, requiring complex analysis, causing infections, necrosis, behavioural changes and mortality). The present study explored the efficacy of passive integrated transponder (PIT) tags for individually marking sea cucumbers by assessing retention rates and long-term side effects of tags inserted in previously unstudied tissues/organs. Individuals of the species Cucumaria frondosa were tagged in the body wall, aquapharyngeal bulb and at the base of the oral tentacles. They were monitored closely for evidence of stress, infection, change in feeding and spawning behaviour and tag retention rate. Implanting the tag in an oral tentacle to reach the hydrovascular system of the aquapharyngeal bulb achieved the best retention rates in full-size individuals: from a maximum of 92% after 30 days to 68% at the end of the experimental period (300 days). Efficacy was lower in smaller individuals (84% after 30 d and 42% after 300 d). Following a slight increase in cloacal movements for 15 h post tagging, no side effect was noted in sea cucumbers tagged in the aquapharyngeal bulb via the tentacles. Feeding and spawning behaviours were not affected and no signs of infections or abnormal cell development in the vicinity of the tags were observed. This study indicates that marking sea cucumbers with 8.2 mm long PIT tags implanted via the oral tentacle is an effective technique, yielding relatively high retention rates over long periods without any detectable physiological or behavioural effects. PMID:26011165

  9. Novel Use of PIT Tags in Sea Cucumbers: Promising Results with the Commercial Species Cucumaria frondosa

    PubMed Central

    Gianasi, Bruno L.; Verkaik, Katie; Hamel, Jean-François; Mercier, Annie

    2015-01-01

    The lack of a reliable and innocuous mark-recapture method has limited studies that would provide essential information for the management of commercial sea cucumbers. Tagging sea cucumbers is notoriously difficult because of their plastic nature and autolysis capacities. The markers that have so far been tested, mainly on or through the body wall, were either lost rapidly or had major drawbacks (e.g. suitable only for batch identification, requiring complex analysis, causing infections, necrosis, behavioural changes and mortality). The present study explored the efficacy of passive integrated transponder (PIT) tags for individually marking sea cucumbers by assessing retention rates and long-term side effects of tags inserted in previously unstudied tissues/organs. Individuals of the species Cucumaria frondosa were tagged in the body wall, aquapharyngeal bulb and at the base of the oral tentacles. They were monitored closely for evidence of stress, infection, change in feeding and spawning behaviour and tag retention rate. Implanting the tag in an oral tentacle to reach the hydrovascular system of the aquapharyngeal bulb achieved the best retention rates in full-size individuals: from a maximum of 92% after 30 days to 68% at the end of the experimental period (300 days). Efficacy was lower in smaller individuals (84% after 30 d and 42% after 300 d). Following a slight increase in cloacal movements for 15 h post tagging, no side effect was noted in sea cucumbers tagged in the aquapharyngeal bulb via the tentacles. Feeding and spawning behaviours were not affected and no signs of infections or abnormal cell development in the vicinity of the tags were observed. This study indicates that marking sea cucumbers with 8.2 mm long PIT tags implanted via the oral tentacle is an effective technique, yielding relatively high retention rates over long periods without any detectable physiological or behavioural effects. PMID:26011165

  10. In Vivo Anti-Cancer Mechanism of Low-Molecular-Weight Fucosylated Chondroitin Sulfate (LFCS) from Sea Cucumber Cucumaria frondosa.

    PubMed

    Liu, Xiaoxiao; Liu, Yong; Hao, Jiejie; Zhao, Xiaoliang; Lang, Yinzhi; Fan, Fei; Cai, Chao; Li, Guoyun; Zhang, Lijuan; Yu, Guangli

    2016-01-01

    The low-molecular-weight fucosylated chondroitin sulfate (LFCS) was prepared from native fucosylated chondroitin sulfate (FCS), which was extracted and isolated from sea cucumber Cucumaria frondosa, and the anti-cancer mechanism of LFCS on mouse Lewis lung carcinoma (LLC) was investigated. The results showed that LFCS remarkably inhibited LLC growth and metastasis in a dose-dependent manner. LFCS induced cell cycle arrest by increasing p53/p21 expression and apoptosis through activation of caspase-3 activity in LLC cells. Meanwhile, LFCS suppressed the expression of vascular endothelial growth factor (VEGF), increased the expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) and downregulated the matrix metalloproteinases (MMPs) level. Furthermore, LFCS significantly suppressed the activation of ERK1/2/p38 MAPK/NF-κB pathway, which played a prime role in expression of MMPs. All of these data indicate LFCS may be used as anti-cancer drug candidates and deserve further study. PMID:27187337

  11. Structure of Sphingolipids From Sea Cucumber Cucumaria frondosa and Structure-Specific Cytotoxicity Against Human HepG2 Cells.

    PubMed

    Jia, Zicai; Song, Yu; Tao, Suyuan; Cong, Peixu; Wang, Xiaoxu; Xue, Changhu; Xu, Jie

    2016-03-01

    To investigate the relationship between structure and activity, three glucocerebroside series (CFC-1, CFC-2 and CFC-3), ceramides (CF-Cer) and long-chain bases (CF-LCB) of sea cucumber Cucumaria frondosa (C. frondosa) were isolated and evaluated in HepG2 cells. The molecular species of CFC-1, CFC-2 and CFC-3 and CF-Cer were identified using reversed-phase liquid chromatography with heated electrospray ionization coupled to high-resolution mass spectrometry (RPLC-HESI-HRMS), and determined on the basis of chemical and spectroscopic evidence: For the three glucocerebroside series, fatty acids (FA) were mainly saturated (18:0 and 22:0), monounsaturated (22:1, 23:1 and 24:1) and 2-hydroxyl FA (2-HFA) (23:1 h and 24:1 h), the structure of long-chain bases (LCB) were dihydroxy (d17:1, d18:1 and d18:2) and trihydroxy (t16:0 and t17:0), and the glycosylation was glucose; For CF-Cer, FA were primarily saturated (17:0) and monounsaturated (16:1 and 19:1), the structure of LCB were dihydroxy (d17:1 and d18:1), and trihydroxy (t16:0). The results of cell experiment indicated that all of three glucocerebroside series, CF-Cer and CF-LCB exhibited an inhibitory effects on cell proliferation. Moreover, CFC-3 was most effective in three glucocerebrosides to HepG-2 cell viability. The inhibition effect of CF-LCB was the strongest, and the inhibition effect of CF-Cer was much stronger than glucocerebrosides. PMID:26861868

  12. Protective effects of new medicinal mushroom, Grifola gargal singer (higher Basidiomycetes), on induced DNA damage in somatic cells of Drosophila melanogaster.

    PubMed

    Postemsky, Pablo Daniel; Palermo, Ana Maria; Curvetto, Néstor Raúl

    2011-01-01

    Grifola gargal is an edible mushroom with attributed antioxidant properties. Different sources of G. gargal materials, i.e., fruit bodies and mycelia grown in liquid or solid media, were used to study its potential protective capacity when somatic mutation and recombination is induced in Drosophila melanogaster using DMBA (7-12-dimethyl-benz(α)anthracene) as promutagen. Heterozygote larvae (white/white+) were grown in media with different concentrations of DMBA. Grifola gargal fruit bodies (GgFB) or mycelia from liquid culture (GgLC) or from solid culture (GgWG), i.e., biotransformed wheat kernel flour, were added to the culture media in combined treatments with DMBA. Water, DMBA solvent, or wheat flour (WF) plus DMBA solvent were used as negative controls. Larval mortality increased from 9% to 11% in negative controls to 31% to 36% in DMBA treatments. The addition of GgFB, GgLC, or GgWG materials produced a protective effect on 25 μmol/vial DMBA-induced mortality. Mutations observed in SMART, as light spots per 100 eyes (LS/100 eyes), increased with increasing doses of DMBA; this was also true when considering the mutation incidence expressed as percentage of eyes exhibiting light spots (% eyes with LS). Interestingly, mycelia from GgFB, GgLC, or GgWG, in the presence of 25 μmol/vial DMBA, showed lower values in SMART of both the total LS/100 eyes and the percentage of eyes with LS. Thus, Grifola gargal materials were not only nontoxic, but in combination with 25 μmol/vial DMBA lowered the mortality induced by the promutagen and showed antimutagenic effects. Protective effects of G. gargal against DMBA are discussed in terms of the onset of desmutagenic and/or bioantimutagenic mechanisms of detoxification in the host organism, probably due to some bioactive compounds known to occur in higher mushrooms. PMID:22181846

  13. Immunomodulatory properties of frondoside A, a major triterpene glycoside from the North Atlantic commercially harvested sea cucumber Cucumaria frondosa.

    PubMed

    Aminin, D L; Agafonova, I G; Kalinin, V I; Silchenko, A S; Avilov, S A; Stonik, V A; Collin, P D; Woodward, C

    2008-09-01

    Frondoside A, a major triterpene glycoside from North Atlantic commercially harvested sea cucumber Cucumaria frondosa, possesses strong immunomodulatory properties in subtoxic doses. Frondoside A stimulates lysosomal activity of mouse macrophages in vivo at a maximal effective stimulatory dose of 0.2 microg per mouse and is maintained over 10 days. This glycoside also shows a 30% stimulation of lysosomal activity in mouse macrophages in vitro at concentrations of 0.1-0.38 microg/mL. Frondoside A enhances macrophage phagocytosis of the bacterium Staphylococcus aureus in vitro at a maximal effective concentration of 0.001 microg/mL. Frondoside A stimulates reactive oxygen species formation in macrophages in vitro at a maximal effective concentration of 0.001 microg/mL. Frondoside A stimulates an increase in the number of antibody plaque-forming cells (normally B-cells in spleen) in vivo with a maximal stimulatory effect at a concentration of 0.2 microg per mouse (stimulatory index, 1.86). Frondoside A has a weak effect upon immunoglobulin (Ig) M production after immunization with sheep erythrocytes in mice. Frondoside A does not stimulate Ig production in mice and does not significantly enhance the ovalbumin-stimulated IgM and IgG antibody levels in ovalbumin-immunized mice. Hence frondoside A is an immunostimulant of cell-based immunity including phagocytosis without a significant effect on amplification of humoral immune activity or adjuvant properties. Therefore, frondoside A may provide curative and/or preventive treatment options against diseases wherein a depleted immune status contributes to the pathological processes. PMID:18800890

  14. TRAINING IN PROBLEM SOLVING.

    ERIC Educational Resources Information Center

    DEVANE, J.R.; RIMOLDI, H.J.A.

    CHANGES WERE STUDIED IN THE PROBLEM-SOLVING BEHAVIOR OF HIGH SCHOOL STUDENTS AS A FUNCTION OF A CAREFULLY DESIGNED TRAINING PROGRAM. TRAINING WAS DEFINED AS THE DEVELOPMENT OF STUDENT AWARENESS OF PROBLEM-SOLVING STRATEGIES USED. INSTRUMENTS WERE DEVELOPED AND REFINED TO MEASURE PROBLEM-SOLVING BEHAVIOR. SPECIFICALLY TESTED WAS THE FOLLOWING…

  15. Isolation of cytotoxic glucoerebrosides and long-chain bases from sea cucumber Cucumaria frondosa using high speed counter-current chromatography.

    PubMed

    Xu, Jie; Guo, Shuang; Du, Lei; Wang, Yu-Ming; Sugawara, Tatsuya; Hirata, Takashi; Xue, Chang-Hu

    2013-01-01

    Total glucocerebrosides of the sea cucumber Cucumaria frondosa (CFC) have been isolated from the less polar lipid fraction of the chloroform-methanol extract using high speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of petroleum ether-methanol-water (5:4:1, v/v). Three glucocerebroside molecular species (CFC-1, CFC-2 and CFC-3) were isolated from crude total cerebrosides with repeated column chromatography. The structures of these three glucocerebroside molecular species were determined on the basis of chemical and spectroscopic evidence: fatty acids were mainly saturated (C22:0 and C18:0), monounsaturated (C24:1 and C20:1) and α-hydroxyl fatty acids (C24:1h, C23:0h, C23:1h and C22:0h), the structures of long-chain bases were dihydroxy (d17:1, d18:2 and d18:1) and trihydroxy (t17:0 and t16:0), and the glycosylation was glucose. High purity long-chain bases of sea cucumber Cucumaria frondosa (CF-LCB) were prepared from total lipids by HSCCC with a two-phase solvent system composed of n-hexane-methyl tert butyl ether-methanol-water (1:1:2:1, v/v). Compare with traditional preparative methods, the method of HSCCC is short cycle, high yield and less solvent consumption. The composition analysis of CF-LCB showed that the ratio of d18:2 and d17:1 was approximately 2:1. The four glucocerebrosides and long-chain bases from sea cucumber Cucumaria frondosa were evaluated for activity in vitro assays for the cytotoxic activities against Caco-2 colon cancer cells. The results indicated that both glucocerebrosides and long-chain bases exhibited an inhibitory effect on cell proliferation. Moreover, CFC-3 was most effective in four glucocerebrosides to Caco-2 cell viability. The inhibition effect of CF-LCB was much stronger than glucocerebrosides. PMID:23470440

  16. Teaching through Problem Solving

    ERIC Educational Resources Information Center

    Fi, Cos D.; Degner, Katherine M.

    2012-01-01

    Teaching through Problem Solving (TtPS) is an effective way to teach mathematics "for" understanding. It also provides students with a way to learn mathematics "with" understanding. In this article, the authors present a definition of what it means to teach through problem solving. They also describe a professional development vignette that…

  17. Chemical Reaction Problem Solving.

    ERIC Educational Resources Information Center

    Veal, William

    1999-01-01

    Discusses the role of chemical-equation problem solving in helping students predict reaction products. Methods for helping students learn this process must be taught to students and future teachers by using pedagogical skills within the content of chemistry. Emphasizes that solving chemical reactions should involve creative cognition where…

  18. Problem Solving and Intelligence.

    ERIC Educational Resources Information Center

    Resnick, Lauren B.; Glaser, Robert

    This paper argues that a major aspect of intelligence is the ability to solve problems and that careful analysis of problem-solving behavior is a means of specifying many of the psychological processes that make up intelligence. The focus is on the mechanisms involved when, in the absence of complete instruction, a person must "invent" a new…

  19. Problem Solving in Nursing.

    ERIC Educational Resources Information Center

    Karns, Phyllis Spear

    The relationship of educational preparation to the problem- solving performance of 55 hospital employed baccalaureate and associate degree nurses working in Wyoming hospitals was studied. Participant data were collected that might correlate with problem-solving ability: age, years of experience in nursing, years of work experience in a…

  20. Techniques of Problem Solving.

    ERIC Educational Resources Information Center

    Krantz, Steven G.

    The purpose of this book is to teach the basic principles of problem solving in both mathematical and non-mathematical problems. The major components of the book consist of learning to translate verbal discussion into analytical data, learning problem solving methods for attacking collections of analytical questions or data, and building a…

  1. Effect of the Medicinal Mushroom, Grifola gargal (Agaricomycetes), on Bone Turnover Markers and Serum Lipids in Middle-Aged and Elderly Japanese Women.

    PubMed

    Harada, Etsuko; Morizono, Toshihiro; Sumiya, Toshimitsu; Kawagishi, Hirokazu

    2016-01-01

    A clinical study was performed to examine the effect of the edible mushroom, Grifola gargal, on bone turnover markers and serum lipids in middle-aged and elderly Japanese women. Postmenopausal women aged 51-73 years (mean age, 61 years) received daily oral administration of 5 g G. gargal fruiting bodies (hot air-dried and powdered; G. gargal powder [GGP]). Serum levels of bone alkaline phosphatase (BAP) and lipids and urinary deoxypyridinoline (DPD) levels were measured before and 2 weeks after the start of GGP treatment. As a result, urinary DPD bone resorption marker levels in women treated with GGP decreased significantly. Serum levels of the BAP bone formation marker also tended to increase, but the difference was not significant. By contrast, the atherogenic index decreased and the high-density lipoprotein (HDL) ratio increased significantly. However, there were no statistically significant differences in serum lipids of total cholesterol, HDL cholesterol, and low-density lipoprotein cholesterol. In addition, this study demonstrated for the first time that G. gargal is safe for human consumption. PMID:27279439

  2. NAEP Note: Problem Solving.

    ERIC Educational Resources Information Center

    Carpenter, Thomas P.; And Others

    1980-01-01

    Student weaknesses on problem-solving portions of the NAEP mathematics assessment are discussed using Polya's heuristics as a framework. Recommendations for classroom instruction are discussed. (MP) Aspect of National Assessment (NAEP) dealt with in this document: Results (Interpretation).

  3. Solving Tommy's Writing Problems.

    ERIC Educational Resources Information Center

    Burdman, Debra

    1986-01-01

    The article describes an approach by which word processing helps to solve some of the writing problems of learning disabled students. Aspects considered include prewriting, drafting, revising, and completing the story. (CL)

  4. Solving Word Problems.

    ERIC Educational Resources Information Center

    Karrison, Joan; Carroll, Margaret Kelly

    1991-01-01

    Students with language and learning disabilities may have difficulty solving mathematics word problems. Use of a sequential checklist, identifying clues and keywords, and illustrating a problem can all help the student identify and implement the correct computational process. (DB)

  5. Problem-Solving Software

    NASA Technical Reports Server (NTRS)

    1992-01-01

    CBR Express software solves problems by adapting sorted solutions to new problems specified by a user. It is applicable to a wide range of situations. The technology was originally developed by Inference Corporation for Johnson Space Center's Advanced Software Development Workstation. The project focused on the reuse of software designs, and Inference used CBR as part of the ACCESS prototype software. The commercial CBR Express is used as a "help desk" for customer support, enabling reuse of existing information when necessary. It has been adopted by several companies, among them American Airlines, which uses it to solve reservation system software problems.

  6. Problem Solving Techniques Seminar.

    ERIC Educational Resources Information Center

    Massachusetts Career Development Inst., Springfield.

    This booklet is one of six texts from a workplace literacy curriculum designed to assist learners in facing the increased demands of the workplace. Six problem-solving techniques are developed in the booklet to assist individuals and groups in making better decisions: problem identification, data gathering, data analysis, solution analysis,…

  7. Circumference and Problem Solving.

    ERIC Educational Resources Information Center

    Blackburn, Katie; White, David

    The concept of pi is one of great importance to all developed civilization and one that can be explored and mastered by elementary students through an inductive and problem-solving approach. Such an approach is outlined and discussed. The approach involves the following biblical quotation: "And he made a moltin sea ten cubits from one brim to the…

  8. Universal Design Problem Solving

    ERIC Educational Resources Information Center

    Sterling, Mary C.

    2004-01-01

    Universal design is made up of four elements: accessibility, adaptability, aesthetics, and affordability. This article addresses the concept of universal design problem solving through experiential learning for an interior design studio course in postsecondary education. Students' experiences with clients over age 55 promoted an understanding of…

  9. What Is Problem Solving?

    ERIC Educational Resources Information Center

    Martinez, Michael E.

    1998-01-01

    Many important human activities involve accomplishing goals without a script. There is no formula for true problem-solving. Heuristic, cognitive "rules of thumb" are the problem-solver's best guide. Learners should understand heuristic tools such as means-end analysis, working backwards, successive approximation, and external representation. Since…

  10. Inquiry and Problem Solving.

    ERIC Educational Resources Information Center

    Thorson, Annette, Ed.

    1999-01-01

    This issue of ENC Focus focuses on the topic of inquiry and problem solving. Featured articles include: (1) "Inquiry in the Everyday World of Schools" (Ronald D. Anderson); (2) "In the Cascade Reservoir Restoration Project Students Tackle Real-World Problems" (Clint Kennedy with Advanced Biology Students from Cascade High School); (3) "Project…

  11. [Problem Solving Activities.

    ERIC Educational Resources Information Center

    Wisconsin Univ. - Stout, Menomonie. Center for Vocational, Technical and Adult Education.

    The teacher directed problem solving activities package contains 17 units: Future Community Design, Let's Build an Elevator, Let's Construct a Catapult, Let's Design a Recreational Game, Let's Make a Hand Fishing Reel, Let's Make a Wall Hanging, Let's Make a Yo-Yo, Marooned in the Past, Metrication, Mousetrap Vehicles, The Multi System…

  12. On Solving Linear Recurrences

    ERIC Educational Resources Information Center

    Dobbs, David E.

    2013-01-01

    A direct method is given for solving first-order linear recurrences with constant coefficients. The limiting value of that solution is studied as "n to infinity." This classroom note could serve as enrichment material for the typical introductory course on discrete mathematics that follows a calculus course.

  13. Electric Current Solves Mazes

    ERIC Educational Resources Information Center

    Ayrinhac, Simon

    2014-01-01

    We present in this work a demonstration of the maze-solving problem with electricity. Electric current flowing in a maze as a printed circuit produces Joule heating and the right way is instantaneously revealed with infrared thermal imaging. The basic properties of electric current can be discussed in this context, with this challenging question:…

  14. Solving Problems in Genetics

    ERIC Educational Resources Information Center

    Aznar, Mercedes Martinez; Orcajo, Teresa Ibanez

    2005-01-01

    A teaching unit on genetics and human inheritance using problem-solving methodology was undertaken with fourth-level Spanish Secondary Education students (15 year olds). The goal was to study certain aspects of the students' learning process (concepts, procedures and attitude) when using this methodology in the school environment. The change…

  15. Problem Solving Using Calculators.

    ERIC Educational Resources Information Center

    Billings, Karen; Moursund, David

    1978-01-01

    The first part in the serialized version of a book on the use of calculators for problem solving is presented. It contains prefaces for teachers and students and a chapter on getting started which includes topics such as symmetries, operations, powers, and chaining. (MP)

  16. Solving Common Mathematical Problems

    NASA Technical Reports Server (NTRS)

    Luz, Paul L.

    2005-01-01

    Mathematical Solutions Toolset is a collection of five software programs that rapidly solve some common mathematical problems. The programs consist of a set of Microsoft Excel worksheets. The programs provide for entry of input data and display of output data in a user-friendly, menu-driven format, and for automatic execution once the input data has been entered.

  17. Problem Solving with Patents

    ERIC Educational Resources Information Center

    Moore, Jerilou; Sumrall, William J.

    2008-01-01

    Exploring our patent system is a great way to engage students in creative problem solving. As a result, the authors designed a teaching unit that uses the study of patents to explore one avenue in which scientists and engineers do science. Specifically, through the development of an idea, students learn how science and technology are connected.…

  18. Solving Problems through Circles

    ERIC Educational Resources Information Center

    Grahamslaw, Laura; Henson, Lisa H.

    2015-01-01

    Several problem-solving interventions that utilise a "circle" approach have been applied within the field of educational psychology, for example, Circle Time, Circle of Friends, Sharing Circles, Circle of Adults and Solution Circles. This research explored two interventions, Solution Circles and Circle of Adults, and used thematic…

  19. Problem Solving in Electricity.

    ERIC Educational Resources Information Center

    Caillot, Michel; Chalouhi, Elias

    Two studies were conducted to describe how students perform direct current (D-C) circuit problems. It was hypothesized that problem solving in the electricity domain depends largely on good visual processing of the circuit diagram and that this processing depends on the ability to recognize when two or more electrical components are in series or…

  20. Mathematics as Problem Solving.

    ERIC Educational Resources Information Center

    Soifer, Alexander

    This book contains about 200 problems. It is suggested that it be used by students, teachers or anyone interested in exploring mathematics. In addition to a general discussion on problem solving, there are problems concerned with number theory, algebra, geometry, and combinatorics. (PK)

  1. Problem Solving by Design

    ERIC Educational Resources Information Center

    Capobianco, Brenda M.; Tyrie, Nancy

    2009-01-01

    In a unique school-university partnership, methods students collaborated with fifth graders to use the engineering design process to build their problem-solving skills. By placing the problem in the context of a client having particular needs, the problem took on a real-world appeal that students found intriguing and inviting. In this article, the…

  2. Fucoidan from sea cucumber Cucumaria frondosa exhibits anti-hyperglycemic effects in insulin resistant mice via activating the PI3K/PKB pathway and GLUT4.

    PubMed

    Wang, Yiming; Wang, Jingfeng; Zhao, Yanlei; Hu, Shiwei; Shi, Di; Xue, Changhu

    2016-01-01

    The present study investigated the anti-hyperglycemic properties and mechanisms of fucoidan, isolated from Cucumaria frondosa (Cf-FUC), in insulin resistant mice. Male C57BL/6J mice were fed regular diet or high-fat/high-sucrose diet for 19 weeks. Model animals were dietary administrated either rosiglitazone (RSG, 1 mg/kg·bw), fucoidan (Cf-FUC, 80 mg/kg·bw) or their combinations. Results showed that Cf-FUC significantly reduced fasting blood glucose and insulin levels, and enhanced glucose tolerance and insulin tolerance in insulin-resistant mice. Quantitative real-time PCR analysis showed that Cf-FUC increased the mRNA expressions of insulin receptors (IR), insulin receptor substrate 1 (IRS-1), phosphatidylinositol 3 kinase (PI3K), protein kinase B (PKB), and glucose transporter 4 (GLUT4). Western blot assays demonstrated that Cf-FUC showed no effect on total protein expression but nevertheless enhanced the phosphorylation of proteins listed above and increased translocation of GLUT4 to the cell membrane. Furthermore, Cf-FUC enhanced the effects of RSG. These results indicated that Cf-FUC exhibited significant anti-hyperglycemic effects via activating PI3K/PKB pathway and GLUT4 in skeletal muscle and adipose tissue. PMID:26194305

  3. Cytotoxic and apoptosis-inducing activity of triterpene glycosides from Holothuria scabra and Cucumaria frondosa against HepG2 cells.

    PubMed

    Wang, Juanjuan; Han, Hua; Chen, Xiangfeng; Yi, Yanghua; Sun, Hongxiang

    2014-08-01

    The cytotoxic effects of thirteen triterpene glycosides from Holothuria scabra Jaeger and Cucumaria frondosa Gunnerus (Holothuroidea) against four human cell lines were detected and their cytotoxicity-structure relationships were established. The apoptosis-inducing activity of a more potent glycoside echinoside A (1) in HepG2 cells was further investigated by determining its effect on the morphology, mitochondrial transmembrane potential (Δψm) and mRNA expression levels of the apoptosis-related genes. The results showed that the number of glycosyl residues in sugar chains and the side chain of aglycone could affect their cytotoxicity towards tumor cells and selective cytotoxicity. 1 significantly inhibited cell viability and induced apoptosis in HepG2 cells. 1 also markedly decreased the Δψm and Bcl-2/Bax mRNA express ratio, and up-regulated the mRNA expression levels of Caspase-3, Caspase-8 and Caspase-9 in HepG2 cells. Therefore, 1 induced apoptosis in HepG2 cells through both intrinsic and extrinsic pathway. These findings could potentially promote the usage of these glycosides as leading compounds for developing new antitumor drugs. PMID:25062508

  4. Cytotoxic and Apoptosis-Inducing Activity of Triterpene Glycosides from Holothuria scabra and Cucumaria frondosa against HepG2 Cells

    PubMed Central

    Wang, Juanjuan; Han, Hua; Chen, Xiangfeng; Yi, Yanghua; Sun, Hongxiang

    2014-01-01

    The cytotoxic effects of thirteen triterpene glycosides from Holothuria scabra Jaeger and Cucumaria frondosa Gunnerus (Holothuroidea) against four human cell lines were detected and their cytotoxicity-structure relationships were established. The apoptosis-inducing activity of a more potent glycoside echinoside A (1) in HepG2 cells was further investigated by determining its effect on the morphology, mitochondrial transmembrane potential (Δψm) and mRNA expression levels of the apoptosis-related genes. The results showed that the number of glycosyl residues in sugar chains and the side chain of aglycone could affect their cytotoxicity towards tumor cells and selective cytotoxicity. 1 significantly inhibited cell viability and induced apoptosis in HepG2 cells. 1 also markedly decreased the Δψm and Bcl-2/Bax mRNA express ratio, and up-regulated the mRNA expression levels of Caspase-3, Caspase-8 and Caspase-9 in HepG2 cells. Therefore, 1 induced apoptosis in HepG2 cells through both intrinsic and extrinsic pathway. These findings could potentially promote the usage of these glycosides as leading compounds for developing new antitumor drugs. PMID:25062508

  5. The WNT/β-catenin pathway is involved in the anti-adipogenic activity of cerebrosides from the sea cucumber Cucumaria frondosa.

    PubMed

    Xu, Hui; Wang, Fei; Wang, Jingfeng; Xu, Jie; Wang, Yuming; Xue, Changhu

    2015-07-01

    Both adipocyte hypertrophy and hyperplasia lead to obesity. Here, we isolated cerebrosides from the sea cucumber Cucumaria frondosa (CFC) and examined its anti-adipogenic activity in vitro. CFC inhibited the lipid accumulation of 3T3-L1 cells and suppressed PPARγ and C/EBPα expressions, proving its anti-adipogenic activity. Furthermore, CFC suppressed lipogenesis in mature adipocytes. The WNT/β-catenin pathway acts as an anti-adipogenic factor. CFC enhanced β-catenin expression, promoted its nuclear translocation and up-regulated the expression of CCND1 and c-myc, two target genes of β-catenin. Moreover, after cells were treated with the β-catenin inhibitor 21H7, β-catenin nuclear translocation and transcription activity can be recovered by CFC. These findings suggested that CFC promoted the activation of the WNT/β-catenin pathway. Besides, CFC enhanced the expressions of Fz1, LRP5 and LRP6, while it had no effect on the expressions of Wnt10b and GSK3β. These findings indicated that CFC exhibits anti-adipogenic activity through enhancing the activation of the WNT/β-catenin pathway, which was mediated by FZ and LRPs. PMID:26091058

  6. Electric current solves mazes

    NASA Astrophysics Data System (ADS)

    Ayrinhac, Simon

    2014-07-01

    We present in this work a demonstration of the maze-solving problem with electricity. Electric current flowing in a maze as a printed circuit produces Joule heating and the right way is instantaneously revealed with infrared thermal imaging. The basic properties of electric current can be discussed in this context, with this challenging question: how can the electric current choose the right way and avoid dead ends?

  7. Solving crimes with hypnosis.

    PubMed

    Wester, William C; Hammond, D Corydon

    2011-04-01

    Following a brief review of the literature on hypnosis and memory, this paper overviews the procedures that are used in conducting forensic hypnosis interviews. Ten forensic hypnosis cases are then described. These real world cases are in stark contrast to research done in an artificial laboratory setting where the information to be recalled lacks personal relevance and was not associated with emotionally arousing situations. These cases illustrate how forensic hypnosis can result in obtaining important additional investigative leads which lead to the solving of crimes. PMID:21598840

  8. Eicosapentaenoic acid-enriched phosphatidylcholine isolated from Cucumaria frondosa exhibits anti-hyperglycemic effects via activating phosphoinositide 3-kinase/protein kinase B signal pathway.

    PubMed

    Hu, Shiwei; Xu, Leilei; Shi, Di; Wang, Jingfeng; Wang, Yuming; Lou, Qiaoming; Xue, Changhu

    2014-04-01

    Eicosapentaenoic acid-enriched phosphatidylcholine was isolated from the sea cucumber Cucumaria frondosa (Cucumaria-PC) and its effects on streptozotocin (STZ)-induced hyperglycemic rats were investigated. Male Sprague-Dawley rats were randomly divided into normal control, model control (STZ), low- and high-dose Cucumaria-PC groups (STZ + Cucumaria-PC at 25 and 75 mg/Kg·b·wt, intragastrically, respectively). Blood glucose, insulin, glycogen in liver and gastrocnemius were determined over 60 days. Insulin signaling in the rats' gastrocnemius was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting. The results showed that Cucumaria-PC significantly decreased blood glucose level, increased insulin secretion and glycogen synthesis in diabetic rats. RT-PCR analysis revealed that Cucumaria-PC significantly promoted the expressions of glycometabolism-related genes of insulin receptor (IR), insulin receptor substrate-1 (IRS-1), phosphoinositide 3-kinase (PI3K), protein kinase B (PKB), and glucose transporter 4 (GLUT4) in gastrocnemius. Western blotting assay demonstrated that Cucumaria-PC remarkably enhanced the proteins abundance of IR-β, PI3K, PKB, GLUT4, as well as phosphorylation of Tyr-IR-β, p85-PI3K, Ser473-PKB (P < 0.05 and P < 0.01). These findings suggested that Cucumaria-PC exhibited significant anti-hyperglycemic activities through up-regulating PI3K/PKB signal pathway mediated by insulin. Nutritional supplementation with Cucumaria-PC, if validated for human studies, may offer an adjunctive therapy for diabetes mellitus. PMID:24168893

  9. The protective effect of eicosapentaenoic acid-enriched phospholipids from sea cucumber Cucumaria frondosa on oxidative stress in PC12 cells and SAMP8 mice.

    PubMed

    Wu, Feng-Juan; Xue, Yong; Liu, Xiao-Fang; Xue, Chang-Hu; Wang, Jing-Feng; Du, Lei; Takahashi, Koretaro; Wang, Yu-Ming

    2014-01-01

    Alzheimer's disease (AD) is a common neurodegenerative disorders, in which oxidative stress plays an important role. The present study investigated the effect of eicosapentaenoic acid-enriched phospholipids (EPA-enriched PL) from the sea cucumber Cucumaria frondosa on oxidative injury in PC12 cells induced by hydrogen peroxide (H2O2) and tert-butylhydroperoxide (t-BHP). We also studied the effect of EPA-enriched PL on learning and memory functions in senescence-accelerated prone mouse strain 8 (SAMP8) in vivo. Pretreatment with EPA-enriched PL resulted in an enhancement of survival in a dose-dependent manner in H2O2 or t-BHP damaged PC12 cells. EPA-enriched PL pretreatment could also reduce the leakage of lactate dehydrogenase (LDH), and increase the intracellular total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity compared with the H2O2 or t-BHP group. The down-regulated Bcl-2 mRNA level and up-regulated Bax, Caspase-9, and Caspase-3 mRNA expression induced by H2O2 or t-BHP could be restored by EPA-enriched PL pretreatment. These results demonstrated that EPA-enriched PL exhibited its neuroprotective effects by virtue of its antioxidant activity, which might be achieved by inhibiting the mitochondria-dependent apoptotic pathway. The neuroprotective effect of EPA-enriched PL was also verified in vivo test: the EPA-enriched PL administration prevented the development of learning and memory impairments in SAMP8 mice. Our results indicated that EPA-enriched PL could offer an efficient and novel strategy to explore novel drugs or functional food for neuronprotection and cognitive improvement. PMID:24231470

  10. Solving Differential Equations in R: Package deSolve

    EPA Science Inventory

    In this paper we present the R package deSolve to solve initial value problems (IVP) written as ordinary differential equations (ODE), differential algebraic equations (DAE) of index 0 or 1 and partial differential equations (PDE), the latter solved using the method of lines appr...

  11. Solving Quasars (Part 1)

    SciTech Connect

    Elvis, Martin

    2003-10-29

    Quasars are a frustratingly unsolved problem in astrophysics. For 40 years quasars and AGN have presented an ever-proliferating list of diverse and confusing phenomenology from optical to X-ray wavelengths. The result has been that despite being the most powerful objects in the universe, quasars have been increasingly sidelined in astrophysics. This is unfortunate, as quasars pose major physics, as well as astrophysics puzzles, and should be important in cosmology. The problem, I believe, is not that we do not know enough physics, but rather that we do not have the right geometry. I have proposed a simple geometric and kinematic model that allows all of the quasar diversity to be understood as part of a single structure, the 'Quasar Atmosphere'. This structure suggests a natural physical interpretation, that builds on our understanding of stars (the only fully solved problem in astrophysics), and offers the prospect that we can use quasars for cosmology at last.

  12. The Identity of Problem Solving

    ERIC Educational Resources Information Center

    Mamona-Downs, Joanna; Downs, Martin

    2005-01-01

    This paper raises issues motivated by considering the "identity" of problem solving. This means that we are concerned with how other mathematics education topics impinge on problem solving, and with themes that naturally arise within the problem-solving agenda. We claim that some of these issues need more attention by educational research, while…

  13. The Problem-Solving Revolution.

    ERIC Educational Resources Information Center

    Bardige, Art

    1983-01-01

    Discusses the use of microcomputers and software as problem-solving tools, including comments on "TK! Solver," automatic problem-solving program (reviewed in detail on pp.84-86 in this same issue). Also discusses problem-solving approaches to bridge the disciplines, such as music/physics, junior high science/mathematics (genetics),…

  14. Solving the Antibiotic Crisis.

    PubMed

    Wright, Gerard D

    2015-02-13

    Antibiotics are essential for both treating and preventing infectious diseases. Paradoxically, despite their importance as pillars of modern medicine, we are in danger of losing antibiotics because of the evolution and dissemination of resistance mechanisms throughout all pathogenic microbes. This fact, coupled with an inability to bring new drugs to market at a pace that matches resistance, has resulted in a crisis of global proportion. Solving this crisis requires the actions of many stakeholders, but chemists, chemical biologists, and microbiologists must drive the scientific innovation that is required to maintain our antibiotic arsenal. This innovation requires (1) a deep understanding of the evolution and reservoirs of resistance; (2) full knowledge of the molecular mechanisms of antibiotic action and resistance; (3) the discovery of chemical and genetic probes of antibiotic action and resistance; (4) the integration of systems biology into antibiotic discovery; and (5) the discovery of new antimicrobial chemical matter. Addressing these pressing scientific gaps will ensure that we can meet the antibiotic crisis with creativity and purpose. PMID:27622298

  15. Computer Problem-Solving Coaches

    NASA Astrophysics Data System (ADS)

    Hsu, Leon; Heller, Kenneth

    2005-09-01

    Computers might be able to play an important role in physics instruction by coaching students to develop good problem-solving skills. Building on previous research on student problem solving and on designing computer programs to teach cognitive skills, we are developing a prototype computer coach to provide students with guided practice in solving problems. In addition to helping students become better problem solvers, such programs can be useful in studying how students learn to solve problems and how and if problem-solving skills can be transferred from a computer to a pencil-and-paper environment.

  16. Chemopreventive effects of Frondanol A5, a Cucumaria frondosa extract, against rat colon carcinogenesis and inhibition of human colon cancer cell growth.

    PubMed

    Janakiram, Naveena B; Mohammed, Altaf; Zhang, Yuting; Choi, Chang-In; Woodward, Carl; Collin, Peter; Steele, Vernon E; Rao, Chinthalapally V

    2010-01-01

    Sea cucumber extracts have been widely used to treat individuals with inflammatory conditions in East Asia. The present study has been designed to test potential colon cancer-preventive properties of Frondanol A5, a glycolipid extract from the sea cucumber, Cucumaria frondosa, using in vivo and in vitro models of colon cancer. Chemopreventive efficacy of Frondanol A5 was evaluated on azoxymethane-induced rat colon carcinogenesis using colonic aberrant crypt foci (ACF) as efficacy marker. At 7 weeks of age, groups of rats (12 per group) were fed the AIN-76A diet, and ACFs were induced by azoxymethane (15 mg/kg body weight). Three days after azoxymethane treatment, rats were fed with the diets containing 0, 150, and 450 ppm of Frondanol A5 and continued on the diets for 8 weeks, at which time ACFs were evaluated. Expression levels of proliferating cell nuclear antigen and p21(WAF1/CIP1) were determined in ACFs. Further, Frondanol A5 (10-120 microg/mL) was studied for its growth-inhibitory and apoptotic effects in the HCT-116 cell line. Dietary administration of 150 and 450 ppm of Frondanol A5 significantly suppressed azoxymethane-induced total colonic ACF formation, approximately 34% to 55% (P < 0.01 to P < 0.0001), and multicrypt aberrant foci (48-68.5%, P < 0.0001) in a dose-dependent manner. ACFs in rats treated with Frondanol A5 showed significant upregulation of p21(WAF1/CIP1) and downregulation of proliferating cell nuclear antigen compared with control group. Frondanol A5 showed growth inhibition at S and G(2)-M phase with a decrease in Cdc25c and an increase in p21(WAF1/CIP) with significant apoptosis associated with H2AX phosphorylation and caspase-2 cleavage in HCT116 cells. Overall, Frondanol A5 exhibits potential chemopreventive properties for colon carcinogenesis, which suggests further development of this sea cucumber extract. PMID:20051375

  17. Parent Problem Solving: Analysis of Problem Solving in Parenthood Transition.

    ERIC Educational Resources Information Center

    Alpert, Judith L.; And Others

    The general purpose of this study was to explore the possibility of adapting the Means-Ends Problem-Solving procedure (MEPS) to the investigation of the individual's transition to parenthood. Specific purposes were to determine (1) the internal consistency of the Parent Problem-Solving Scale (PPSS), of its subclasses, and of a combined subscale;…

  18. Pulsating Star Mystery Solved

    NASA Astrophysics Data System (ADS)

    2010-11-01

    By discovering the first double star where a pulsating Cepheid variable and another star pass in front of one another, an international team of astronomers has solved a decades-old mystery. The rare alignment of the orbits of the two stars in the double star system has allowed a measurement of the Cepheid mass with unprecedented accuracy. Up to now astronomers had two incompatible theoretical predictions of Cepheid masses. The new result shows that the prediction from stellar pulsation theory is spot on, while the prediction from stellar evolution theory is at odds with the new observations. The new results, from a team led by Grzegorz Pietrzyński (Universidad de Concepción, Chile, Obserwatorium Astronomiczne Uniwersytetu Warszawskiego, Poland), appear in the 25 November 2010 edition of the journal Nature. Grzegorz Pietrzyński introduces this remarkable result: "By using the HARPS instrument on the 3.6-metre telescope at ESO's La Silla Observatory in Chile, along with other telescopes, we have measured the mass of a Cepheid with an accuracy far greater than any earlier estimates. This new result allows us to immediately see which of the two competing theories predicting the masses of Cepheids is correct." Classical Cepheid Variables, usually called just Cepheids, are unstable stars that are larger and much brighter than the Sun [1]. They expand and contract in a regular way, taking anything from a few days to months to complete the cycle. The time taken to brighten and grow fainter again is longer for stars that are more luminous and shorter for the dimmer ones. This remarkably precise relationship makes the study of Cepheids one of the most effective ways to measure the distances to nearby galaxies and from there to map out the scale of the whole Universe [2]. Unfortunately, despite their importance, Cepheids are not fully understood. Predictions of their masses derived from the theory of pulsating stars are 20-30% less than predictions from the theory of the

  19. Multiple Ways to Solve Proportions

    ERIC Educational Resources Information Center

    Ercole, Leslie K.; Frantz, Marny; Ashline, George

    2011-01-01

    When solving problems involving proportions, students may intuitively draw on strategies that connect to their understanding of fractions, decimals, and percents. These two statements--"Instruction in solving proportions should include methods that have a strong intuitive basis" and "Teachers should begin instruction with more intuitive…

  20. Learning Impasses in Problem Solving

    NASA Technical Reports Server (NTRS)

    Hodgson, J. P. E.

    1992-01-01

    Problem Solving systems customarily use backtracking to deal with obstacles that they encounter in the course of trying to solve a problem. This paper outlines an approach in which the possible obstacles are investigated prior to the search for a solution. This provides a solution strategy that avoids backtracking.

  1. Problem Solving, Scaffolding and Learning

    ERIC Educational Resources Information Center

    Lin, Shih-Yin

    2012-01-01

    Helping students to construct robust understanding of physics concepts and develop good solving skills is a central goal in many physics classrooms. This thesis examine students' problem solving abilities from different perspectives and explores strategies to scaffold students' learning. In studies involving analogical problem solving…

  2. Creative Thinking and Problem Solving.

    ERIC Educational Resources Information Center

    Lacy, Grace

    The booklet considers the nature of creativity in children and examines classroom implications. Among the topics addressed are the following: theories about creativity; research; developments in brain research; the creative process; creative problem solving; the Structure of Intellect Problem Solving (SIPS) model; a rationale for creativity in the…

  3. Problem Solving in the Professions.

    ERIC Educational Resources Information Center

    Jackling, Noel; And Others

    1990-01-01

    It is proposed that algorithms and heuristics are useful in improving professional problem-solving abilities when contextualized within the academic discipline. A basic algorithm applied to problem solving in undergraduate engineering education and a similar algorithm applicable to legal problems are used as examples. Problem complexity and…

  4. Problem Solving vis Soap Bubbles

    ERIC Educational Resources Information Center

    Bader, William A.

    1975-01-01

    Describes the use of a scientific phenomenon related to the concept of surface tension as an intriguing vehicle to direct attention to useful problem solving techniques. The need for a definite building process in attempts to solve mathematical problems is stressed. (EB)

  5. Contextual Problem Solving Model Origination

    ERIC Educational Resources Information Center

    Ernst, Jeremy V.

    2009-01-01

    Problem solving has become a central focus of instructional activity in technology education classrooms at all levels (Boser, 1993). Impact assessment considerations incorporating society, culture, and economics are factors that require high-level deliberation involving critical thinking and the implementation of problem solving strategy. The…

  6. Difficulties in Genetics Problem Solving.

    ERIC Educational Resources Information Center

    Tolman, Richard R.

    1982-01-01

    Examined problem-solving strategies of 30 high school students as they solved genetics problems. Proposes a new sequence of teaching genetics based on results: meiosis, sex chromosomes, sex determination, sex-linked traits, monohybrid and dihybrid crosses (humans), codominance (humans), and Mendel's pea experiments. (JN)

  7. A Method for Solving Problems.

    ERIC Educational Resources Information Center

    Knoop, Robert

    1987-01-01

    Problem solving and decision making are considered to be keys to successful management. A normative method for problem solving is presented, suggesting that the analysis of the problem be structured along a five-step procedure: problem identification, analysis, decision alternatives, decision making, and decision implementation. Follow-up…

  8. Problem Solving with General Semantics.

    ERIC Educational Resources Information Center

    Hewson, David

    1996-01-01

    Discusses how to use general semantics formulations to improve problem solving at home or at work--methods come from the areas of artificial intelligence/computer science, engineering, operations research, and psychology. (PA)

  9. Robot, computer problem solving system

    NASA Technical Reports Server (NTRS)

    Becker, J. D.

    1972-01-01

    The development of a computer problem solving system is reported that considers physical problems faced by an artificial robot moving around in a complex environment. Fundamental interaction constraints with a real environment are simulated for the robot by visual scan and creation of an internal environmental model. The programming system used in constructing the problem solving system for the simulated robot and its simulated world environment is outlined together with the task that the system is capable of performing. A very general framework for understanding the relationship between an observed behavior and an adequate description of that behavior is included.

  10. Quantitative Reasoning in Problem Solving

    ERIC Educational Resources Information Center

    Ramful, Ajay; Ho, Siew Yin

    2015-01-01

    In this article, Ajay Ramful and Siew Yin Ho explain the meaning of quantitative reasoning, describing how it is used in the to solve mathematical problems. They also describe a diagrammatic approach to represent relationships among quantities and provide examples of problems and their solutions.

  11. Customer Service & Team Problem Solving.

    ERIC Educational Resources Information Center

    Martin, Sabrina Budasi

    This curriculum guide provides materials for a six-session, site-specific training course in customer service and team problem solving for the Claretian Medical Center. The course outline is followed the six lesson plans. Components of each lesson plan include a list of objectives, an outline of activities and discussion topics for the lesson,…

  12. Robot computer problem solving system

    NASA Technical Reports Server (NTRS)

    Becker, J. D.; Merriam, E. W.

    1974-01-01

    The conceptual, experimental, and practical aspects of the development of a robot computer problem solving system were investigated. The distinctive characteristics were formulated of the approach taken in relation to various studies of cognition and robotics. Vehicle and eye control systems were structured, and the information to be generated by the visual system is defined.

  13. Solving Problems by Reading Mathematics.

    ERIC Educational Resources Information Center

    Witkowski, Joseph C.

    1988-01-01

    A course at the University of Georgia is described that helps students acquire problem-solving skills so that ultimately the entire remedial program would be improved, giving students with major deficiencies in basic skills a better chance to succeed in their regular university courses. (MLW)

  14. Robot computer problem solving system

    NASA Technical Reports Server (NTRS)

    Becker, J. D.; Merriam, E. W.

    1974-01-01

    The conceptual, experimental, and practical phases of developing a robot computer problem solving system are outlined. Robot intelligence, conversion of the programming language SAIL to run under the THNEX monitor, and the use of the network to run several cooperating jobs at different sites are discussed.

  15. Problem-Solving Test: Pyrosequencing

    ERIC Educational Resources Information Center

    Szeberenyi, Jozsef

    2013-01-01

    Terms to be familiar with before you start to solve the test: Maxam-Gilbert sequencing, Sanger sequencing, gel electrophoresis, DNA synthesis reaction, polymerase chain reaction, template, primer, DNA polymerase, deoxyribonucleoside triphosphates, orthophosphate, pyrophosphate, nucleoside monophosphates, luminescence, acid anhydride bond,…

  16. Supporting Problem Solving in PBL

    ERIC Educational Resources Information Center

    Jonassen, David

    2011-01-01

    Although the characteristics of PBL (problem focused, student centered, self-directed, etc.) are well known, the components of a problem-based learning environment (PBLE) and the cognitive scaffolds necessary to support learning to solve different kinds of problems with different learners is less clear. This paper identifies the different…

  17. Problem Solving through Paper Folding

    ERIC Educational Resources Information Center

    Wares, Arsalan

    2014-01-01

    The purpose of this article is to describe a couple of challenging mathematical problems that involve paper folding. These problem-solving tasks can be used to foster geometric and algebraic thinking among students. The context of paper folding makes some of the abstract mathematical ideas involved relatively concrete. When implemented…

  18. Students' Problem Solving and Justification

    ERIC Educational Resources Information Center

    Glass, Barbara; Maher, Carolyn A.

    2004-01-01

    This paper reports on methods of students' justifications of their solution to a problem in the area of combinatorics. From the analysis of the problem solving of 150 students in a variety of settings from high-school to graduate study, four major forms of reasoning evolved: (1) Justification by Cases, (2) Inductive Argument, (3) Elimination…

  19. Time Out for Problem Solving.

    ERIC Educational Resources Information Center

    Champagne, Audrey B.; And Others

    Teachers in elementary schools, supervisors of instruction, and other educational practitioners are the primary audience for this publication. The paper presents philosophical, psychological, and practical reasons for including a problem-solving approach in elementary school instruction. It draws on the writings of John Dewey, Jean Piaget, James…

  20. Genetics problem solving and worldview

    NASA Astrophysics Data System (ADS)

    Dale, Esther

    The research goal was to determine whether worldview relates to traditional and real-world genetics problem solving. Traditionally, scientific literacy emphasized content knowledge alone because it was sufficient to solve traditional problems. The contemporary definition of scientific literacy is, "The knowledge and understanding of scientific concepts and processes required for personal decision-making, participation in civic and cultural affairs and economic productivity" (NRC, 1996). An expanded definition of scientific literacy is needed to solve socioscientific issues (SSI), complex social issues with conceptual, procedural, or technological associations with science. Teaching content knowledge alone assumes that students will find the scientific explanation of a phenomenon to be superior to a non-science explanation. Formal science and everyday ways of thinking about science are two different cultures (Palmer, 1999). Students address this rift with cognitive apartheid, the boxing away of science knowledge from other types of knowledge (Jedege & Aikenhead, 1999). By addressing worldview, cognitive apartheid may decrease and scientific literacy may increase. Introductory biology students at the University of Minnesota during fall semester 2005 completed a written questionnaire-including a genetics content-knowledge test, four genetic dilemmas, the Worldview Assessment Instrument (WAI) and some items about demographics and religiosity. Six students responded to the interview protocol. Based on statistical analysis and interview data, this study concluded the following: (1) Worldview, in the form of metaphysics, relates to solving traditional genetic dilemmas. (2) Worldview, in the form of agency, relates to solving traditional genetics problems. (3) Thus, worldview must be addressed in curriculum, instruction, and assessment.

  1. Quantum Computing: Solving Complex Problems

    ScienceCinema

    DiVincenzo, David [IBM Watson Research Center

    2009-09-01

    One of the motivating ideas of quantum computation was that there could be a new kind of machine that would solve hard problems in quantum mechanics. There has been significant progress towards the experimental realization of these machines (which I will review), but there are still many questions about how such a machine could solve computational problems of interest in quantum physics. New categorizations of the complexity of computational problems have now been invented to describe quantum simulation. The bad news is that some of these problems are believed to be intractable even on a quantum computer, falling into a quantum analog of the NP class. The good news is that there are many other new classifications of tractability that may apply to several situations of physical interest.

  2. An NOy* Algorithm for SOLVE

    NASA Technical Reports Server (NTRS)

    Loewenstein, M.; Greenblatt. B. J.; Jost, H.; Podolske, J. R.; Elkins, Jim; Hurst, Dale; Romanashkin, Pavel; Atlas, Elliott; Schauffler, Sue; Donnelly, Steve; Condon, Estelle (Technical Monitor)

    2000-01-01

    De-nitrification and excess re-nitrification was widely observed by ER-2 instruments in the Arctic vortex during SOLVE in winter/spring 2000. Analyses of these events requires a knowledge of the initial or pre-vortex state of the sampled air masses. The canonical relationship of NOy to the long-lived tracer N2O observed in the unperturbed stratosphere is generally used for this purpose. In this paper we will attempt to establish the current unperturbed NOy:N2O relationship (NOy* algorithm) using the ensemble of extra-vortex data from in situ instruments flying on the ER-2 and DC-8, and from the Mark IV remote measurements on the OMS balloon. Initial analysis indicates a change in the SOLVE NOy* from the values predicted by the 1994 Northern Hemisphere NOy* algorithm which was derived from the observations in the ASHOE/MAESA campaign.

  3. Quantum Computing: Solving Complex Problems

    SciTech Connect

    DiVincenzo, David

    2007-04-12

    One of the motivating ideas of quantum computation was that there could be a new kind of machine that would solve hard problems in quantum mechanics. There has been significant progress towards the experimental realization of these machines (which I will review), but there are still many questions about how such a machine could solve computational problems of interest in quantum physics. New categorizations of the complexity of computational problems have now been invented to describe quantum simulation. The bad news is that some of these problems are believed to be intractable even on a quantum computer, falling into a quantum analog of the NP class. The good news is that there are many other new classifications of tractability that may apply to several situations of physical interest.

  4. Quantum Computing: Solving Complex Problems

    SciTech Connect

    DiVincenzo, David

    2007-04-11

    One of the motivating ideas of quantum computation was that there could be a new kind of machine that would solve hard problems in quantum mechanics. There has been significant progress towards the experimental realization of these machines (which I will review), but there are still many questions about how such a machine could solve computational problems of interest in quantum physics. New categorizations of the complexity of computational problems have now been invented to describe quantum simulation. The bad news is that some of these problems are believed to be intractable even on a quantum computer, falling into a quantum analog of the NP class. The good news is that there are many other new classifications of tractability that may apply to several situations of physical interest.

  5. Maze Solving by Chemotactic Droplets

    SciTech Connect

    Lagzi, Istvan; Soh, Siowling; Wesson, Paul J.; Browne, Kevin P.; Grzybowski, Bartosz A.

    2010-01-11

    Droplets emitting surface-active chemicals exhibit chemotaxis toward low-pH regions. Such droplets are self-propelled and navigate through a complex maze to seek a source of acid placed at one of the maze’s exits. In doing so, the droplets find the shortest path through the maze. Chemotaxis and maze solving are due to an interplay between acid/base chemistry and surface tension effects.

  6. Journey toward Teaching Mathematics through Problem Solving

    ERIC Educational Resources Information Center

    Sakshaug, Lynae E.; Wohlhuter, Kay A.

    2010-01-01

    Teaching mathematics through problem solving is a challenge for teachers who learned mathematics by doing exercises. How do teachers develop their own problem solving abilities as well as their abilities to teach mathematics through problem solving? A group of teachers began the journey of learning to teach through problem solving while taking a…

  7. Problem Solving in the Context of Medicine.

    ERIC Educational Resources Information Center

    Woods, Donald R.

    1997-01-01

    Reviews the book "Medical Problem Solving: An Analysis of Clinical Reasoning," a seminal book whose conclusions on problem solving in medical fields are still valid today. Discusses major problem-solving findings of this book, the application of the findings to education, and relating knowledge to problem-solving skills. (JRH)

  8. Assessing Algebraic Solving Ability: A Theoretical Framework

    ERIC Educational Resources Information Center

    Lian, Lim Hooi; Yew, Wun Thiam

    2012-01-01

    Algebraic solving ability had been discussed by many educators and researchers. There exists no definite definition for algebraic solving ability as it can be viewed from different perspectives. In this paper, the nature of algebraic solving ability in terms of algebraic processes that demonstrate the ability in solving algebraic problem is…

  9. Solving the Dark Matter Problem

    ScienceCinema

    Baltz, Ted

    2009-09-01

    Cosmological observations have firmly established that the majority of matter in the universe is of an unknown type, called 'dark matter'. A compelling hypothesis is that the dark matter consists of weakly interacting massive particles (WIMPs) in the mass range around 100 GeV. If the WIMP hypothesis is correct, such particles could be created and studied at accelerators. Furthermore they could be directly detected as the primary component of our galaxy. Solving the dark matter problem requires that the connection be made between the two. We describe some theoretical and experimental avenues that might lead to this connection.

  10. Solving Equations of Multibody Dynamics

    NASA Technical Reports Server (NTRS)

    Jain, Abhinandan; Lim, Christopher

    2007-01-01

    Darts++ is a computer program for solving the equations of motion of a multibody system or of a multibody model of a dynamic system. It is intended especially for use in dynamical simulations performed in designing and analyzing, and developing software for the control of, complex mechanical systems. Darts++ is based on the Spatial-Operator- Algebra formulation for multibody dynamics. This software reads a description of a multibody system from a model data file, then constructs and implements an efficient algorithm that solves the dynamical equations of the system. The efficiency and, hence, the computational speed is sufficient to make Darts++ suitable for use in realtime closed-loop simulations. Darts++ features an object-oriented software architecture that enables reconfiguration of system topology at run time; in contrast, in related prior software, system topology is fixed during initialization. Darts++ provides an interface to scripting languages, including Tcl and Python, that enable the user to configure and interact with simulation objects at run time.

  11. Modeling Applied to Problem Solving

    NASA Astrophysics Data System (ADS)

    Pawl, Andrew; Barrantes, Analia; Pritchard, David E.

    2009-11-01

    We describe a modeling approach to help students learn expert problem solving. Models are used to present and hierarchically organize the syllabus content and apply it to problem solving, but students do not develop and validate their own Models through guided discovery. Instead, students classify problems under the appropriate instructor-generated Model by selecting a system to consider and describing the interactions that are relevant to that system. We believe that this explicit System, Interactions and Model (S.I.M.) problem modeling strategy represents a key simplification and clarification of the widely disseminated modeling approach originated by Hestenes and collaborators. Our narrower focus allows modeling physics to be integrated into (as opposed to replacing) a typical introductory college mechanics course, while preserving the emphasis on understanding systems and interactions that is the essence of modeling. We have employed the approach in a three-week review course for MIT freshmen who received a D in the fall mechanics course with very encouraging results.

  12. SOLV-DB: Solvents Data

    DOE Data Explorer

    SOLV-DB provides a specialized mix of information on commercially available solvents. The development of the database was funded under the Strategic Environmental Research and Development Program (SERDP) with funds from EPA and DOE's Office of Industrial Technologies in EE. The information includes: • Health and safety considerations involved in choosing and using solvents • Chemical and physical data affecting the suitability of a particular solvent for a wide range of potential applications • Regulatory responsibilities, including exposure and effluent limits, hazard classification status with respect to several key statutes, and selected reporting requirements • Environmental fate data, to indicate whether a solvent is likely to break down or persist in air or water, and what types of waste treatment techniques may apply to it • CAS numbers (from Chemical Abstracts Service) and Sax Numbers (from Sax, et.al., Dangerous Properties of Industrial Materials) Supplier Information See help information at http://solvdb.ncms.org/welcome.htm (Specialized Interface)

  13. Robot computer problem solving system

    NASA Technical Reports Server (NTRS)

    Merriam, E. W.; Becker, J. D.

    1973-01-01

    A robot computer problem solving system which represents a robot exploration vehicle in a simulated Mars environment is described. The model exhibits changes and improvements made on a previously designed robot in a city environment. The Martian environment is modeled in Cartesian coordinates; objects are scattered about a plane; arbitrary restrictions on the robot's vision have been removed; and the robot's path contains arbitrary curves. New environmental features, particularly the visual occlusion of objects by other objects, were added to the model. Two different algorithms were developed for computing occlusion. Movement and vision capabilities of the robot were established in the Mars environment, using LISP/FORTRAN interface for computational efficiency. The graphical display program was redesigned to reflect the change to the Mars-like environment.

  14. Maze-solving by chemotaxis

    NASA Astrophysics Data System (ADS)

    Reynolds, A. M.

    2010-06-01

    Here, we report on numerical simulations showing that chemotaxis will take a body through a maze via the shortest possible route to the source of a chemoattractant. This is a robust finding that does not depend on the geometrical makeup of the maze. The predictions are supported by recent experimental studies which have shown that by moving down gradients in pH , a droplet of organic solvent can find the shortest of multiple possible paths through a maze to an acid-soaked exit. They are also consistent with numerical and experimental evidence that plant-parasitic nematodes take the shortest route through the labyrinth of air-filled pores within soil to preferred host plants that produce volatile chemoattractants. The predictions support the view that maze-solving is a robust property of chemotaxis and is not specific to particular kinds of maze or to the fractal structure of air-filled channels within soils.

  15. Solving the structure of metakaolin

    SciTech Connect

    Proffen, Thomas E; White, Claire E; Provis, John L; Riley, Daniel P; Van Deventer, Jannie S J

    2009-01-01

    Metakaolin has been used extensively as a cement additive and paint extender, and recently as a geopolymer precursor. This disordered layered aluminosilicate is formed via the dehydroxylation of kaolinite. However, an accurate representation of its atomic structure has bever before been presented. Here, a novel synergy between total scattering and density functional modeling is presented to solve the structure of metakaolin. The metastable structure is elucidated by iterating between least-squares real-space refinement using neutron pair distribution function data, and geometry optimization using density functional modeling. The resulting structural representation is both energetically feasible and in excellent agreement with experimental data. This accurate structure of metakaolin provides new insight into the local environment of the aluminum atoms, with evidence of the existence of tri-coordinated aluminum. By the availability of this detailed atomic description, there exists the opportunity to tailor chemical and mechanical processes involving metakaolin at the atomic level to obtain optimal performance at the macro-scale.

  16. Problem Solving in the General Mathematics Classroom

    ERIC Educational Resources Information Center

    Troutman, Andria Price; Lichtenberg, Betty Plunkett

    1974-01-01

    Five steps common to different problem solving models are listed. Next, seven specific abilities related to solving problems are discussed and examples given. Sample activities, appropriate to help in developing these specific abilities, are suggested. (LS)

  17. Using Logo to Develop Problem Solving Skills.

    ERIC Educational Resources Information Center

    Denenberg, Stewart A.

    1993-01-01

    Proposes using computer programing teaching problem solving. Describes the problem-solving technique of Top-Down Design, discusses its application to LOGO, and provides examples of programs using LOGO. (MDH)

  18. Developing Creativity through Collaborative Problem Solving

    ERIC Educational Resources Information Center

    Albert, Lillie R.; Kim, Rina

    2013-01-01

    This paper discusses an alternative approach for developing problem solving experiences for students. The major argument is that students can develop their creativity by engaging in collaborative problem solving activities in which they apply a variety of mathematical methods creatively to solve problems. The argument is supported by: considering…

  19. King Oedipus and the Problem Solving Process.

    ERIC Educational Resources Information Center

    Borchardt, Donald A.

    An analysis of the problem solving process reveals at least three options: (1) finding the cause, (2) solving the problem, and (3) anticipating potential problems. These methods may be illustrated by examining "Oedipus Tyrannus," a play in which a king attempts to deal with a problem that appears to be beyond his ability to solve, and applying…

  20. Disciplinary Foundations for Solving Interdisciplinary Scientific Problems

    ERIC Educational Resources Information Center

    Zhang, Dongmei; Shen, Ji

    2015-01-01

    Problem-solving has been one of the major strands in science education research. But much of the problem-solving research has been conducted on discipline-based contexts; little research has been done on how students, especially individuals, solve interdisciplinary problems. To understand how individuals reason about interdisciplinary problems, we…

  1. Some Applications of Algebraic System Solving

    ERIC Educational Resources Information Center

    Roanes-Lozano, Eugenio

    2011-01-01

    Technology and, in particular, computer algebra systems, allows us to change both the way we teach mathematics and the mathematical curriculum. Curiously enough, unlike what happens with linear system solving, algebraic system solving is not widely known. The aim of this paper is to show that, although the theory lying behind the "exact solve"…

  2. Research on Computers and Problem Solving.

    ERIC Educational Resources Information Center

    Burton, John K.; And Others

    1988-01-01

    Eight articles review and report on research involving computers and problem solving skills. Topics discussed include research design; problem solving skills and programing languages, including BASIC and LOGO; computer anxiety; diagnostic programs for arithmetic problems; and relationships between ability and problem solving scores and between…

  3. Theoretical and Philosophical Perspectives to Problem Solving.

    ERIC Educational Resources Information Center

    Sherman, Thomas M.; And Others

    1988-01-01

    Five articles explore various theoretical aspects of problems and problem solving skills. Highlights include strategies to learn problem solving skills; knowledge structures; metacognition; behavioral processes and cognitive psychology; erotetic logic; creativity as an aspect of computer problem solving; and programing as a problem-solving…

  4. Perspectives on Problem Solving and Instruction

    ERIC Educational Resources Information Center

    van Merrienboer, Jeroen J. G.

    2013-01-01

    Most educators claim that problem solving is important, but they take very different perspective on it and there is little agreement on how it should be taught. This article aims to sort out the different perspectives and discusses problem solving as a goal, a method, and a skill. As a goal, problem solving should not be limited to well-structured…

  5. Collis-Romberg Mathematical Problem Solving Profiles.

    ERIC Educational Resources Information Center

    Collis, K. F.; Romberg, T. A.

    Problem solving has become a focus of mathematics programs in Australia in recent years, necessitating the assessment of students' problem-solving abilities. This manual provides a problem-solving assessment and teaching resource package containing four elements: (1) profiles assessment items; (2) profiles diagnostic forms for recording individual…

  6. Affect and Problem Solving: Two Theoretical Perspectives.

    ERIC Educational Resources Information Center

    McLeod, Douglas B.

    Cognitive factors related to problem solving have been explored, but affective factors also play an important role in the teaching of mathematical problem solving. This paper outlines the theories of George Mandler and Bernard Weiner, providing a useful background for research related to affect and problem solving. Data related to the two theories…

  7. Kindergarten Students Solving Mathematical Word Problems

    ERIC Educational Resources Information Center

    Johnson, Nickey Owen

    2013-01-01

    The purpose of this study was to explore problem solving with kindergarten students. This line of inquiry is highly significant given that Common Core State Standards emphasize deep, conceptual understanding in mathematics as well as problem solving in kindergarten. However, there is little research on problem solving with kindergarten students.…

  8. LEGO Robotics: An Authentic Problem Solving Tool?

    ERIC Educational Resources Information Center

    Castledine, Alanah-Rei; Chalmers, Chris

    2011-01-01

    With the current curriculum focus on correlating classroom problem solving lessons to real-world contexts, are LEGO robotics an effective problem solving tool? This present study was designed to investigate this question and to ascertain what problem solving strategies primary students engaged with when working with LEGO robotics and whether the…

  9. Community-powered problem solving.

    PubMed

    Gouillart, Francis; Billings, Douglas

    2013-04-01

    Traditionally, companies have managed their constituencies with specific processes: marketing to customers, procuring from vendors, developing HR policies for employees, and so on. The problem is, such processes focus on repeatability and compliance, so they can lead to stagnation. Inviting your constituencies to collectively help you solve problems and exploit opportunities--"co-creation"--is a better approach. It allows you to continually tap the skills and insights of huge numbers of stakeholders and develop new ways to produce value for all. The idea is to provide stakeholders with platforms (physical and digital forums) on which they can interact, get them to start exploring new experiences and connections, and let the system grow organically. A co-creation initiative by a unit of Becton, Dickinson and Company demonstrates how this works. A global leader in syringes, BD set out to deepen its ties with hospital customers and help them reduce the incidence of infections from unsafe injection and syringe disposal practices. The effort began with a cross-functional internal team, brought in the hospital procurement and supply managers BD had relationships with, and then reached out to hospitals' infection-prevention and occupational health leaders. Eventually product designers, nurses, sustainability staffers, and even hospital CFOs were using the platform, contributing data that generated new best practices and reduced infections. PMID:23593769

  10. Distance Measurement Solves Astrophysical Mysteries

    NASA Astrophysics Data System (ADS)

    2003-08-01

    Location, location, and location. The old real-estate adage about what's really important proved applicable to astrophysics as astronomers used the sharp radio "vision" of the National Science Foundation's Very Long Baseline Array (VLBA) to pinpoint the distance to a pulsar. Their accurate distance measurement then resolved a dispute over the pulsar's birthplace, allowed the astronomers to determine the size of its neutron star and possibly solve a mystery about cosmic rays. "Getting an accurate distance to this pulsar gave us a real bonanza," said Walter Brisken, of the National Radio Astronomy Observatory (NRAO) in Socorro, NM. Monogem Ring The Monogem Ring, in X-Ray Image by ROSAT satellite CREDIT: Max-Planck Institute, American Astronomical Society (Click on Image for Larger Version) The pulsar, called PSR B0656+14, is in the constellation Gemini, and appears to be near the center of a circular supernova remnant that straddles Gemini and its neighboring constellation, Monoceros, and is thus called the Monogem Ring. Since pulsars are superdense, spinning neutron stars left over when a massive star explodes as a supernova, it was logical to assume that the Monogem Ring, the shell of debris from a supernova explosion, was the remnant of the blast that created the pulsar. However, astronomers using indirect methods of determining the distance to the pulsar had concluded that it was nearly 2500 light-years from Earth. On the other hand, the supernova remnant was determined to be only about 1000 light-years from Earth. It seemed unlikely that the two were related, but instead appeared nearby in the sky purely by a chance juxtaposition. Brisken and his colleagues used the VLBA to make precise measurements of the sky position of PSR B0656+14 from 2000 to 2002. They were able to detect the slight offset in the object's apparent position when viewed from opposite sides of Earth's orbit around the Sun. This effect, called parallax, provides a direct measurement of

  11. Analog Processor To Solve Optimization Problems

    NASA Technical Reports Server (NTRS)

    Duong, Tuan A.; Eberhardt, Silvio P.; Thakoor, Anil P.

    1993-01-01

    Proposed analog processor solves "traveling-salesman" problem, considered paradigm of global-optimization problems involving routing or allocation of resources. Includes electronic neural network and auxiliary circuitry based partly on concepts described in "Neural-Network Processor Would Allocate Resources" (NPO-17781) and "Neural Network Solves 'Traveling-Salesman' Problem" (NPO-17807). Processor based on highly parallel computing solves problem in significantly less time.

  12. Solving global optimization problems on GPU cluster

    NASA Astrophysics Data System (ADS)

    Barkalov, Konstantin; Gergel, Victor; Lebedev, Ilya

    2016-06-01

    The paper contains the results of investigation of a parallel global optimization algorithm combined with a dimension reduction scheme. This allows solving multidimensional problems by means of reducing to data-independent subproblems with smaller dimension solved in parallel. The new element implemented in the research consists in using several graphic accelerators at different computing nodes. The paper also includes results of solving problems of well-known multiextremal test class GKLS on Lobachevsky supercomputer using tens of thousands of GPU cores.

  13. Mobile serious games for collaborative problem solving.

    PubMed

    Sanchez, Jaime; Mendoza, Claudia; Salinas, Alvaro

    2009-01-01

    This paper presents the results obtained from the implementation of a series of learning activities based on mobile serious games (MSG) for the development of problem-solving and collaborative skills in Chilean 8th grade students. Three MSGs were developed and played by teams of four students, who had to solve the problems posed by the game collaboratively. The data shows that the experimental group had a higher perception of their own skills of collaboration and of the plan execution dimension of problem solving than the control group, providing empirical evidence regarding the contribution of MSGs to the development of collaborative problem-solving skills. PMID:19592762

  14. Fibonacci's Triangle: A Vehicle for Problem Solving.

    ERIC Educational Resources Information Center

    Ouellette, Hugh

    1979-01-01

    A method for solving certain types of problems is illustrated by problems related to Fibonacci's triangle. The method involves pattern recognition, generalizing, algebraic manipulation, and mathematical induction. (MP)

  15. Applications of Symmetry to Problem Solving.

    ERIC Educational Resources Information Center

    Leikin, Roza; Berman, Abraham; Zaslavsky, Orit

    2000-01-01

    Symmetry is an important mathematical concept that plays an extremely important role as a problem solving technique. Presents examples of problems from several branches of mathematics that can be solved using different types of symmetry. Discusses teachers' attitudes and beliefs regarding the use of symmetry in the solutions of these problems.…

  16. Student Modeling Based on Problem Solving Times

    ERIC Educational Resources Information Center

    Pelánek, Radek; Jarušek, Petr

    2015-01-01

    Student modeling in intelligent tutoring systems is mostly concerned with modeling correctness of students' answers. As interactive problem solving activities become increasingly common in educational systems, it is useful to focus also on timing information associated with problem solving. We argue that the focus on timing is natural for certain…

  17. Metacognition: Student Reflections on Problem Solving

    ERIC Educational Resources Information Center

    Wismath, Shelly; Orr, Doug; Good, Brandon

    2014-01-01

    Twenty-first century teaching and learning focus on the fundamental skills of critical thinking and problem solving, creativity and innovation, and collaboration and communication. Metacognition is a crucial aspect of both problem solving and critical thinking, but it is often difficult to get students to engage in authentic metacognitive…

  18. Distributed problem solving by pilots and dispatchers

    NASA Technical Reports Server (NTRS)

    Orasanu, Judith; Wich, Mike; Fischer, Ute; Jobe, Kim; Mccoy, Elaine; Beatty, Roger; Smith, Phil

    1993-01-01

    The study addressed the following question: Are flight planning problems solved differently by PILOTS and DISPATCHERS when they work alone versus when they work together? Aspect of their performance that were of interest include the following: Problem perception and definition; Problem solving strategies and information use; Options considered; Solution and rational; and errors.

  19. Mathematical Problem Solving through Sequential Process Analysis

    ERIC Educational Resources Information Center

    Codina, A.; Cañadas, M. C.; Castro, E.

    2015-01-01

    Introduction: The macroscopic perspective is one of the frameworks for research on problem solving in mathematics education. Coming from this perspective, our study addresses the stages of thought in mathematical problem solving, offering an innovative approach because we apply sequential relations and global interrelations between the different…

  20. General Description of Human Problem Solving.

    ERIC Educational Resources Information Center

    Klein, Gary A.; Weitzenfeld, Julian

    A theoretical model relating problem identification to problem solving is presented. The main purpose of the study is to increase understanding of decision making among Air Force educators. The problem-solving process is defined as the generation and evaluation of alternatives that will accomplish what is needed and the reidentification of what is…

  1. Problem Solving and Technology. ACESIA Monograph 2.

    ERIC Educational Resources Information Center

    Lomon, Earle L.; And Others

    1977-01-01

    The two articles dealing with problem solving and technology in this publication should be useful to those developing the kinds of materials, experiences, and thinking that elementary school industrial arts offers children. The first article accepts problem solving as an educational goal and reports a timely and universally acceptable approach.…

  2. Geographic Information Systems: Implications for Problem Solving.

    ERIC Educational Resources Information Center

    Audet, Richard H.; Abegg, Gerald L.

    1996-01-01

    Compares expert-/novice-based problem-solving behaviors with a Geographic Information Systems program. Uses naturalistic methods to analyze problem-solving strategies for occurrence of thematic elements. Reports that experts relied on logical formulations to query the database while novices used trial-and-error methods and midlevel cognitive…

  3. Children Solving Problems. The Developing Child Series.

    ERIC Educational Resources Information Center

    Thornton, Stephanie

    The developmental increase in the ability to solve problems is a puzzle. Does it come from basic changes in mental skills, or is it a matter of practice? This book from the Developing Child series synthesizes recent research examining children's problem-solving skills development. Chapter 1 presents the major themes: (1) there is increasing…

  4. Dynamic Problem Solving: A New Assessment Perspective

    ERIC Educational Resources Information Center

    Greiff, Samuel; Wustenberg, Sascha; Funke, Joachim

    2012-01-01

    This article addresses two unsolved measurement issues in dynamic problem solving (DPS) research: (a) unsystematic construction of DPS tests making a comparison of results obtained in different studies difficult and (b) use of time-intensive single tasks leading to severe reliability problems. To solve these issues, the MicroDYN approach is…

  5. Could HPS Improve Problem-Solving?

    ERIC Educational Resources Information Center

    Coelho, Ricardo Lopes

    2013-01-01

    It is generally accepted nowadays that History and Philosophy of Science (HPS) is useful in understanding scientific concepts, theories and even some experiments. Problem-solving strategies are a significant topic, since students' careers depend on their skill to solve problems. These are the reasons for addressing the question of whether problem…

  6. Problem Solving Interactions on Electronic Networks.

    ERIC Educational Resources Information Center

    Waugh, Michael; And Others

    Arguing that electronic networking provides a medium which is qualitatively superior to the traditional classroom for conducting certain types of problem solving exercises, this paper details the Water Problem Solving Project, which was conducted on the InterCultural Learning Network in 1985 and 1986 with students from the United States, Mexico,…

  7. Taking "From Scratch" out of Problem Solving

    ERIC Educational Resources Information Center

    Brown, Wayne

    2007-01-01

    Solving problems and creating processes and procedures from the ground up has long been part of the IT department's way of operating. IT staffs will continue to encounter new problems to solve and new technologies to be implemented. They also must involve their constituents in the creation of solutions. Nonetheless, for many issues they no longer…

  8. A Problem-Solving Model for Instruction.

    ERIC Educational Resources Information Center

    Scott, Roger O.

    This second in a series of three papers on the Associated Staff Training Program of the Foreign Language Innovative Curriculum Study concentrates on the problem solving strategy employed by the program's specially trained innovative agents--the Instructional Systems Consultants (ISC). The problem-solving method used is first illustrated by citing…

  9. Error Detection Processes in Problem Solving.

    ERIC Educational Resources Information Center

    Allwood, Carl Martin

    1984-01-01

    Describes a study which analyzed problem solvers' error detection processes by instructing subjects to think aloud when solving statistical problems. Effects of evaluative episodes on error detection, detection of different error types, error detection processes per se, and relationship of error detection behavior to problem-solving proficiency…

  10. Conceptual Problem Solving in High School Physics

    ERIC Educational Resources Information Center

    Docktor, Jennifer L.; Strand, Natalie E.; Mestre, José P.; Ross, Brian H.

    2015-01-01

    Problem solving is a critical element of learning physics. However, traditional instruction often emphasizes the quantitative aspects of problem solving such as equations and mathematical procedures rather than qualitative analysis for selecting appropriate concepts and principles. This study describes the development and evaluation of an…

  11. Solving Problems in Genetics II: Conceptual Restructuring

    ERIC Educational Resources Information Center

    Orcajo, Teresa Ibanez; Aznar, Mercedes Martinez

    2005-01-01

    This paper presents the results of part of an investigation carried out with fourth-level Spanish secondary education students (15 years old), in which we implemented a teaching unit based on problem-solving methodology as an investigation to teach genetics and human inheritance curricular contents. By solving open problems, the students…

  12. Teaching Top-Down Problem Solving.

    ERIC Educational Resources Information Center

    Patrick, Charles

    Top-down problem solving is a methodical approach to obtaining real solutions for open-ended problems common in the realms of engineering and science. The technique provides a means for logically understanding a problem prior to attempting a solution. Steps in the top-down problem-solving method include the following: (1) identifying a need; (2)…

  13. Problem Solving Software for Math Classes.

    ERIC Educational Resources Information Center

    Troutner, Joanne

    1987-01-01

    Described are 10 computer software programs for problem solving related to mathematics. Programs described are: (1) Box Solves Story Problems; (2) Safari Search; (3) Puzzle Tanks; (4) The King's Rule; (5) The Factory; (6) The Royal Rules; (7) The Enchanted Forest; (8) Gears; (9) The Super Factory; and (10) Creativity Unlimited. (RH)

  14. Solving Problems with Charts & Tables. Pipefitter.

    ERIC Educational Resources Information Center

    Greater Baton Rouge Chamber of Commerce, LA.

    Developed as part of the ABCs of Construction National Workplace Literacy Project, this instructional module is designed to help individuals employed as pipefitters learn to solve problems with charts and tables. Outlined in the first section is a five-step procedure for solving problems involving tables and/or charts: identifying the question to…

  15. Problem Solving Software: What Does It Teach?

    ERIC Educational Resources Information Center

    Duffield, Judith A.

    The purpose of this study was to examine the potential of computer-assisted instruction (CAI) for teaching problem solving skills. It was conducted in three phases. During the first phase, two pieces of problem solving software, "The King's Rule" and "Safari Search," were identified and analyzed. During the second phase, two groups of six…

  16. Beyond Computation: Improving Mathematical Problem Solving.

    ERIC Educational Resources Information Center

    Anderson, Jennifer M.; Olson, Jennifer S.; Wrobel, Margaret L.

    This action research describes a program for improving mathematical problem solving skills. The targeted population consisted of first grade students in a transient, middle class community as well as third and sixth grade students from a growing, middle to upper class in Illinois. The concerns of problem solving were documented through teacher…

  17. Parallel Algorithm Solves Coupled Differential Equations

    NASA Technical Reports Server (NTRS)

    Hayashi, A.

    1987-01-01

    Numerical methods adapted to concurrent processing. Algorithm solves set of coupled partial differential equations by numerical integration. Adapted to run on hypercube computer, algorithm separates problem into smaller problems solved concurrently. Increase in computing speed with concurrent processing over that achievable with conventional sequential processing appreciable, especially for large problems.

  18. Measuring Problem Solving Skills in "Portal 2"

    ERIC Educational Resources Information Center

    Shute, Valerie J.; Wang, Lubin

    2013-01-01

    This paper examines possible improvement to problem solving skills as a function of playing the video game "Portal 2." Stealth assessment is used in the game to evaluate students' problem solving abilities--specifically basic and flexible rule application. The stealth assessment measures will be validated against commonly accepted…

  19. Developing Legal Problem-Solving Skills.

    ERIC Educational Resources Information Center

    Nathanson, Stephen

    1994-01-01

    A law professor explains how he came to view legal problem solving as the driving concept in law school curriculum design and draws on personal experience and a survey of students concerning teaching methods in a commercial law course. He outlines six curriculum design principles for teaching legal problem solving. (MSE)

  20. Solving Cubic Equations by Polynomial Decomposition

    ERIC Educational Resources Information Center

    Kulkarni, Raghavendra G.

    2011-01-01

    Several mathematicians struggled to solve cubic equations, and in 1515 Scipione del Ferro reportedly solved the cubic while participating in a local mathematical contest, but did not bother to publish his method. Then it was Cardano (1539) who first published the solution to the general cubic equation in his book "The Great Art, or, The Rules of…

  1. A Multivariate Model of Physics Problem Solving

    ERIC Educational Resources Information Center

    Taasoobshirazi, Gita; Farley, John

    2013-01-01

    A model of expertise in physics problem solving was tested on undergraduate science, physics, and engineering majors enrolled in an introductory-level physics course. Structural equation modeling was used to test hypothesized relationships among variables linked to expertise in physics problem solving including motivation, metacognitive planning,…

  2. Problem solving in health services organizations.

    PubMed

    Rakich, J S; Krigline, A B

    1996-01-01

    Health services organization managers at all levels are constantly confronted with problems. Conditions encountered that initiate the need for problem solving are opportunity, threat, crisis, deviation, and improvement. A general problem-solving model presenting an orderly process by which managers can approach this important task is described. An example of the model applied to the current strategic climate is presented. PMID:10158720

  3. Teaching and Learning through Problem Solving

    ERIC Educational Resources Information Center

    Ollerton, Mike

    2007-01-01

    In this article, the author relates some problem solving work with primary schools to Department for Children, Schools, and Families (DfES) support. In four primary schools in the West Midlands, the focus was teaching mathematics through problem solving, based on materials published on the DfES "standards" website. The author noticed the way…

  4. Super 7: Daily Exercises in Problem Solving.

    ERIC Educational Resources Information Center

    Hamilton, Octavia

    This book is a year-long program of daily exercises in problem solving for 2nd and 3rd grade students that presents 144 lessons, each with seven problems. The problems cover number sense, computation, measurements, geometry, problem solving, and patterns. The material is presented in a sequential fashion with concepts repeated and expanded, and…

  5. Enigma of Runaway Stars Solved

    NASA Astrophysics Data System (ADS)

    1997-01-01

    Supernova Propels Companion Star through Interstellar Space The following success story is a classical illustration of scientific progress through concerted interplay of observation and theory. It concerns a 35-year old mystery which has now been solved by means of exciting observations of a strange double star. An added touch is the successive involvement of astronomers connected to the European Southern Observatory. For many years, astronomers have been puzzled by the fact that, among the thousands of very young, hot and heavy stars which have been observed in the Milky Way, there are some that move with exceptionally high velocities. In some cases, motions well above 100 km/sec, or ten times more than normal for such stars, have been measured. How is this possible? Which mechanism is responsible for the large amounts of energy needed to move such heavy bodies at such high speeds? Could it be that these stars are accelerated during the powerful explosion of a companion star as a supernova? Such a scenario was proposed in 1961 by Adriaan Blaauw [1], but until now, observational proof has been lacking. Now, however, strong supporting evidence for this mechanism has become available from observations obtained at the ESO La Silla observatory. The mysterious runaway stars OB-runaway stars [2] are heavy stars that travel through interstellar space with an anomalously high velocity. They have been known for several decades, but it has always been a problem to explain their high velocities. Although most OB-runaway stars are located at distances of several thousands of lightyears, their high velocity results in a measurable change in position on sky photos taken several years apart. The velocity component in the direction of the Earth can be measured very accurately from a spectrogram. From a combination of such observations, it is possible to measure the space velocity of OB-runaways. Bow shocks reveal runaway stars It has also been found that some OB-runaways display

  6. [Antiviral properties of basidiomycetes metabolites].

    PubMed

    Avtonomova, A V; Krasnopolskaya, L M

    2014-01-01

    The data on the antiviral action of the Ganoderma lucidum, Lentinus edodes, Grifola frondosa, Agaricus brasiliensis and other basidiomycetes metabolites are summurized. The metabolites of these species of basidiomycetes exhibit a direct antiviral effect on herpes simplex virus types I and II, human immunodeficiency virus (HIV), hepatitis B virus, vesicular stomatitis virus, influenza virus, Epstein-Barr virus, and others. Moreover, metabolites of basidiomycetes increased antiviral immunity. PMID:25975107

  7. WickSolve Version 1.0

    SciTech Connect

    Andraka, Charles E.

    2011-09-27

    WickSolve is a code used to design wick structures for sodium heat pipe receivers for dish Stirling systems. WickSolve can model distributed pore size wicks in domes and cylinders, and interfaces with CIRCE2, another Sandia code, to determine flux boundary conditions to the wick. WickSolve is available as an executable for Windows computer systems. Wicksolve is used for design of wick structures for high temperature sodium heat pipes for solar applications. These wick structures may contain a distribution of pore sizes (felt metal, sintered metal powders, etc) to enhance heat transfer by allowing partial wick dryout. WickSolve incorporates a 2-D liquid flow model (finite differencing) to model in-plane liquid flow, and a 1-D vapor flow finite difference model for vapor flow perpendicular to the liquid flow surface. The models are coupled by the liquid void fraction in the wick, and solved iteratively.

  8. WickSolve Version 1.0

    Energy Science and Technology Software Center (ESTSC)

    2011-09-27

    WickSolve is a code used to design wick structures for sodium heat pipe receivers for dish Stirling systems. WickSolve can model distributed pore size wicks in domes and cylinders, and interfaces with CIRCE2, another Sandia code, to determine flux boundary conditions to the wick. WickSolve is available as an executable for Windows computer systems. Wicksolve is used for design of wick structures for high temperature sodium heat pipes for solar applications. These wick structures maymore » contain a distribution of pore sizes (felt metal, sintered metal powders, etc) to enhance heat transfer by allowing partial wick dryout. WickSolve incorporates a 2-D liquid flow model (finite differencing) to model in-plane liquid flow, and a 1-D vapor flow finite difference model for vapor flow perpendicular to the liquid flow surface. The models are coupled by the liquid void fraction in the wick, and solved iteratively.« less

  9. Pre-Service Class Teacher' Ability in Solving Mathematical Problems and Skills in Solving Daily Problems

    ERIC Educational Resources Information Center

    Aljaberi, Nahil M.; Gheith, Eman

    2016-01-01

    This study aims to investigate the ability of pre-service class teacher at University of Petrain solving mathematical problems using Polya's Techniques, their level of problem solving skills in daily-life issues. The study also investigates the correlation between their ability to solve mathematical problems and their level of problem solving…

  10. The Cyclic Nature of Problem Solving: An Emergent Multidimensional Problem-Solving Framework

    ERIC Educational Resources Information Center

    Carlson, Marilyn P.; Bloom, Irene

    2005-01-01

    This paper describes the problem-solving behaviors of 12 mathematicians as they completed four mathematical tasks. The emergent problem-solving framework draws on the large body of research, as grounded by and modified in response to our close observations of these mathematicians. The resulting "Multidimensional Problem-Solving Framework" has four…

  11. Disciplinary Foundations for Solving Interdisciplinary Scientific Problems

    NASA Astrophysics Data System (ADS)

    Zhang, Dongmei; Shen, Ji

    2015-10-01

    Problem-solving has been one of the major strands in science education research. But much of the problem-solving research has been conducted on discipline-based contexts; little research has been done on how students, especially individuals, solve interdisciplinary problems. To understand how individuals reason about interdisciplinary problems, we conducted an interview study with 16 graduate students coming from a variety of disciplinary backgrounds. During the interviews, we asked participants to solve two interdisciplinary science problems on the topic of osmosis. We investigated participants' problem reasoning processes and probed in their attitudes toward general interdisciplinary approach and specific interdisciplinary problems. Through a careful inductive content analysis of their responses, we studied how disciplinary, cognitive, and affective factors influenced their interdisciplinary problems-solving. We found that participants' prior discipline-based science learning experiences had both positive and negative influences on their interdisciplinary problem-solving. These influences were embodied in their conceptualization of the interdisciplinary problems, the strategies they used to integrate different disciplinary knowledge, and the attitudes they had toward interdisciplinary approach in general and specific interdisciplinary problems. This study sheds light on interdisciplinary science education by revealing the complex relationship between disciplinary learning and interdisciplinary problem-solving.

  12. Lesion mapping of social problem solving.

    PubMed

    Barbey, Aron K; Colom, Roberto; Paul, Erick J; Chau, Aileen; Solomon, Jeffrey; Grafman, Jordan H

    2014-10-01

    Accumulating neuroscience evidence indicates that human intelligence is supported by a distributed network of frontal and parietal regions that enable complex, goal-directed behaviour. However, the contributions of this network to social aspects of intellectual function remain to be well characterized. Here, we report a human lesion study (n = 144) that investigates the neural bases of social problem solving (measured by the Everyday Problem Solving Inventory) and examine the degree to which individual differences in performance are predicted by a broad spectrum of psychological variables, including psychometric intelligence (measured by the Wechsler Adult Intelligence Scale), emotional intelligence (measured by the Mayer, Salovey, Caruso Emotional Intelligence Test), and personality traits (measured by the Neuroticism-Extraversion-Openness Personality Inventory). Scores for each variable were obtained, followed by voxel-based lesion-symptom mapping. Stepwise regression analyses revealed that working memory, processing speed, and emotional intelligence predict individual differences in everyday problem solving. A targeted analysis of specific everyday problem solving domains (involving friends, home management, consumerism, work, information management, and family) revealed psychological variables that selectively contribute to each. Lesion mapping results indicated that social problem solving, psychometric intelligence, and emotional intelligence are supported by a shared network of frontal, temporal, and parietal regions, including white matter association tracts that bind these areas into a coordinated system. The results support an integrative framework for understanding social intelligence and make specific recommendations for the application of the Everyday Problem Solving Inventory to the study of social problem solving in health and disease. PMID:25070511

  13. Lesion mapping of social problem solving

    PubMed Central

    Colom, Roberto; Paul, Erick J.; Chau, Aileen; Solomon, Jeffrey; Grafman, Jordan H.

    2014-01-01

    Accumulating neuroscience evidence indicates that human intelligence is supported by a distributed network of frontal and parietal regions that enable complex, goal-directed behaviour. However, the contributions of this network to social aspects of intellectual function remain to be well characterized. Here, we report a human lesion study (n = 144) that investigates the neural bases of social problem solving (measured by the Everyday Problem Solving Inventory) and examine the degree to which individual differences in performance are predicted by a broad spectrum of psychological variables, including psychometric intelligence (measured by the Wechsler Adult Intelligence Scale), emotional intelligence (measured by the Mayer, Salovey, Caruso Emotional Intelligence Test), and personality traits (measured by the Neuroticism-Extraversion-Openness Personality Inventory). Scores for each variable were obtained, followed by voxel-based lesion–symptom mapping. Stepwise regression analyses revealed that working memory, processing speed, and emotional intelligence predict individual differences in everyday problem solving. A targeted analysis of specific everyday problem solving domains (involving friends, home management, consumerism, work, information management, and family) revealed psychological variables that selectively contribute to each. Lesion mapping results indicated that social problem solving, psychometric intelligence, and emotional intelligence are supported by a shared network of frontal, temporal, and parietal regions, including white matter association tracts that bind these areas into a coordinated system. The results support an integrative framework for understanding social intelligence and make specific recommendations for the application of the Everyday Problem Solving Inventory to the study of social problem solving in health and disease. PMID:25070511

  14. Assertiveness and problem solving in midwives

    PubMed Central

    Yurtsal, Zeliha Burcu; Özdemir, Levent

    2015-01-01

    Background: Midwifery profession is required to bring solutions to problems and a midwife is expected to be an assertive person and to develop midwifery care. This study was planned to examine the relationship between assertiveness and problem-solving skills of midwives. Materials and Methods: This cross-sectional study was conducted with 201 midwives between July 2008 and February 2009 in the city center of Sivas. The Rathus Assertiveness Schedule (RAS) and Problem Solving Inventory (PSI) were used to determine the level of assertiveness and problem-solving skills of midwives. Statistical methods were used as mean, standard deviation, percentage, Student's T, ANOVA and Tukey HSD, Kruskal Wallis, Fisher Exact, Pearson Correlation and Chi-square tests and P < 0.05. Results: The RAS mean scores and the PSI mean scores showed statistically significant differences in terms of a midwife's considering herself as a member of the health team, expressing herself within the health care team, being able to say “no” when necessary, cooperating with her colleagues, taking part in problem-solving skills training. A statistically significant negative correlation was found between the RAS and PSI scores. The RAS scores decreased while the problem-solving scores increased (r: -0451, P < 0.01). Conclusions: There were significant statistical differences between assertiveness levels and problem solving skills of midwives, and midwives who were assertive solved their problems better than did others. Assertiveness and problem-solving skills training will contribute to the success of the midwifery profession. Midwives able to solve problems, and display assertive behaviors will contribute to the development of midwifery profession. PMID:26793247

  15. Solving the Schroedinger equation using Smolyak interpolants

    SciTech Connect

    Avila, Gustavo; Carrington, Tucker Jr.

    2013-10-07

    In this paper, we present a new collocation method for solving the Schroedinger equation. Collocation has the advantage that it obviates integrals. All previous collocation methods have, however, the crucial disadvantage that they require solving a generalized eigenvalue problem. By combining Lagrange-like functions with a Smolyak interpolant, we device a collocation method that does not require solving a generalized eigenvalue problem. We exploit the structure of the grid to develop an efficient algorithm for evaluating the matrix-vector products required to compute energy levels and wavefunctions. Energies systematically converge as the number of points and basis functions are increased.

  16. Using Problem Solving to Teach the Disabled.

    ERIC Educational Resources Information Center

    Cardon, Phillip L.; Scott, Michael L.

    2000-01-01

    Technology education can and should play a role in the development of ideas through problem solving to assist teachers of students with disabilities. Technology teachers can adapt the Engineering for Success model in working with these students. (JOW)

  17. Organizational Structure and Complex Problem Solving

    ERIC Educational Resources Information Center

    Becker, Selwyn W.; Baloff, Nicholas

    1969-01-01

    The problem-solving efficiency of different organization structures is discussed in relation to task requirements and the appropriate organizational behavior, to group adaptation to a task over time, and to various group characteristics. (LN)

  18. Physics: Quantum problems solved through games

    NASA Astrophysics Data System (ADS)

    Maniscalco, Sabrina

    2016-04-01

    Humans are better than computers at performing certain tasks because of their intuition and superior visual processing. Video games are now being used to channel these abilities to solve problems in quantum physics. See Letter p.210

  19. Spatial visualization in physics problem solving.

    PubMed

    Kozhevnikov, Maria; Motes, Michael A; Hegarty, Mary

    2007-07-01

    Three studies were conducted to examine the relation of spatial visualization to solving kinematics problems that involved either predicting the two-dimensional motion of an object, translating from one frame of reference to another, or interpreting kinematics graphs. In Study 1, 60 physics-naíve students were administered kinematics problems and spatial visualization ability tests. In Study 2, 17 (8 high- and 9 low-spatial ability) additional students completed think-aloud protocols while they solved the kinematics problems. In Study 3, the eye movements of fifteen (9 high- and 6 low-spatial ability) students were recorded while the students solved kinematics problems. In contrast to high-spatial students, most low-spatial students did not combine two motion vectors, were unable to switch frames of reference, and tended to interpret graphs literally. The results of the study suggest an important relationship between spatial visualization ability and solving kinematics problems with multiple spatial parameters. PMID:21635308

  20. Problem-solving test: Tryptophan operon mutants.

    PubMed

    Szeberényi, József

    2010-09-01

    Terms to be familiar with before you start to solve the test: tryptophan, operon, operator, repressor, inducer, corepressor, promoter, RNA polymerase, chromosome-polysome complex, regulatory gene, cis-acting element, trans-acting element, plasmid, transformation. PMID:21567855

  1. Problem Solving, Patterns, Probability, Pascal, and Palindromes.

    ERIC Educational Resources Information Center

    Hylton-Lindsay, Althea Antoinette

    2003-01-01

    Presents a problem-solving activity, the birth order problem, and several solution-seeking strategies. Includes responses of current and prospective teachers and a comparison of various strategies. (YDS)

  2. Neural Network Solves "Traveling-Salesman" Problem

    NASA Technical Reports Server (NTRS)

    Thakoor, Anilkumar P.; Moopenn, Alexander W.

    1990-01-01

    Experimental electronic neural network solves "traveling-salesman" problem. Plans round trip of minimum distance among N cities, visiting every city once and only once (without backtracking). This problem is paradigm of many problems of global optimization (e.g., routing or allocation of resources) occuring in industry, business, and government. Applied to large number of cities (or resources), circuits of this kind expected to solve problem faster and more cheaply.

  3. Conceptual problem solving in high school physics

    NASA Astrophysics Data System (ADS)

    Docktor, Jennifer L.; Strand, Natalie E.; Mestre, José P.; Ross, Brian H.

    2015-12-01

    Problem solving is a critical element of learning physics. However, traditional instruction often emphasizes the quantitative aspects of problem solving such as equations and mathematical procedures rather than qualitative analysis for selecting appropriate concepts and principles. This study describes the development and evaluation of an instructional approach called Conceptual Problem Solving (CPS) which guides students to identify principles, justify their use, and plan their solution in writing before solving a problem. The CPS approach was implemented by high school physics teachers at three schools for major theorems and conservation laws in mechanics and CPS-taught classes were compared to control classes taught using traditional problem solving methods. Information about the teachers' implementation of the approach was gathered from classroom observations and interviews, and the effectiveness of the approach was evaluated from a series of written assessments. Results indicated that teachers found CPS easy to integrate into their curricula, students engaged in classroom discussions and produced problem solutions of a higher quality than before, and students scored higher on conceptual and problem solving measures.

  4. Could HPS Improve Problem-Solving?

    NASA Astrophysics Data System (ADS)

    Coelho, Ricardo Lopes

    2013-05-01

    It is generally accepted nowadays that History and Philosophy of Science (HPS) is useful in understanding scientific concepts, theories and even some experiments. Problem-solving strategies are a significant topic, since students' careers depend on their skill to solve problems. These are the reasons for addressing the question of whether problem solving could be improved by means of HPS. Three typical problems in introductory courses of mechanics—the inclined plane, the simple pendulum and the Atwood machine—are taken as the object of the present study. The solving strategies of these problems in the eighteenth and nineteenth century constitute the historical component of the study. Its philosophical component stems from the foundations of mechanics research literature. The use of HPS leads us to see those problems in a different way. These different ways can be tested, for which experiments are proposed. The traditional solving strategies for the incline and pendulum problems are adequate for some situations but not in general. The recourse to apparent weights in the Atwood machine problem leads us to a new insight and a solving strategy for composed Atwood machines. Educational implications also concern the development of logical thinking by means of the variety of lines of thought provided by HPS.

  5. Innovative problem solving by wild spotted hyenas

    PubMed Central

    Benson-Amram, Sarah; Holekamp, Kay E.

    2012-01-01

    Innovative animals are those able to solve novel problems or invent novel solutions to existing problems. Despite the important ecological and evolutionary consequences of innovation, we still know very little about the traits that vary among individuals within a species to make them more or less innovative. Here we examine innovative problem solving by spotted hyenas (Crocuta crocuta) in their natural habitat, and demonstrate for the first time in a non-human animal that those individuals exhibiting a greater diversity of initial exploratory behaviours are more successful problem solvers. Additionally, as in earlier work, we found that neophobia was a critical inhibitor of problem-solving success. Interestingly, although juveniles and adults were equally successful in solving the problem, juveniles were significantly more diverse in their initial exploratory behaviours, more persistent and less neophobic than were adults. We found no significant effects of social rank or sex on success, the diversity of initial exploratory behaviours, behavioural persistence or neophobia. Our results suggest that the diversity of initial exploratory behaviours, akin to some measures of human creativity, is an important, but largely overlooked, determinant of problem-solving success in non-human animals. PMID:22874748

  6. Innovative problem solving by wild spotted hyenas.

    PubMed

    Benson-Amram, Sarah; Holekamp, Kay E

    2012-10-01

    Innovative animals are those able to solve novel problems or invent novel solutions to existing problems. Despite the important ecological and evolutionary consequences of innovation, we still know very little about the traits that vary among individuals within a species to make them more or less innovative. Here we examine innovative problem solving by spotted hyenas (Crocuta crocuta) in their natural habitat, and demonstrate for the first time in a non-human animal that those individuals exhibiting a greater diversity of initial exploratory behaviours are more successful problem solvers. Additionally, as in earlier work, we found that neophobia was a critical inhibitor of problem-solving success. Interestingly, although juveniles and adults were equally successful in solving the problem, juveniles were significantly more diverse in their initial exploratory behaviours, more persistent and less neophobic than were adults. We found no significant effects of social rank or sex on success, the diversity of initial exploratory behaviours, behavioural persistence or neophobia. Our results suggest that the diversity of initial exploratory behaviours, akin to some measures of human creativity, is an important, but largely overlooked, determinant of problem-solving success in non-human animals. PMID:22874748

  7. Why students still can't solve physics problems after solving over 2000 problems

    NASA Astrophysics Data System (ADS)

    Byun, Taejin; Lee, Gyoungho

    2014-09-01

    This study investigates the belief that solving a large number of physics problems helps students better learn physics. We investigated the number of problems solved, student confidence in solving these problems, academic achievement, and the level of conceptual understanding of 49 science high school students enrolled in upper-level physics classes from Spring 2010 to Summer 2011. The participants solved an average of 2200 physics problems before entering high school. Despite having solved so many problems, no statistically significant correlation was found between the number of problems solved and academic achievement on either a mid-term or physics competition examination. In addition, no significant correlation was found between the number of physics problems solved and performance on the Force Concept Inventory (FCI). Lastly, four students were selected from the 49 participants with varying levels of experience and FCI scores for a case study. We determined that their problem solving and learning strategies was more influential in their success than the number of problems they had solved.

  8. AI tools in computer based problem solving

    NASA Technical Reports Server (NTRS)

    Beane, Arthur J.

    1988-01-01

    The use of computers to solve value oriented, deterministic, algorithmic problems, has evolved a structured life cycle model of the software process. The symbolic processing techniques used, primarily in research, for solving nondeterministic problems, and those for which an algorithmic solution is unknown, have evolved a different model, much less structured. Traditionally, the two approaches have been used completely independently. With the advent of low cost, high performance 32 bit workstations executing identical software with large minicomputers and mainframes, it became possible to begin to merge both models into a single extended model of computer problem solving. The implementation of such an extended model on a VAX family of micro/mini/mainframe systems is described. Examples in both development and deployment of applications involving a blending of AI and traditional techniques are given.

  9. Solving inversion problems with neural networks

    NASA Technical Reports Server (NTRS)

    Kamgar-Parsi, Behzad; Gualtieri, J. A.

    1990-01-01

    A class of inverse problems in remote sensing can be characterized by Q = F(x), where F is a nonlinear and noninvertible (or hard to invert) operator, and the objective is to infer the unknowns, x, from the observed quantities, Q. Since the number of observations is usually greater than the number of unknowns, these problems are formulated as optimization problems, which can be solved by a variety of techniques. The feasibility of neural networks for solving such problems is presently investigated. As an example, the problem of finding the atmospheric ozone profile from measured ultraviolet radiances is studied.

  10. Problem solving in a distributed environment

    NASA Technical Reports Server (NTRS)

    Rashid, R. F.

    1980-01-01

    Distributed problem solving is anayzed as a blend of two disciplines: (1) problem solving and ai; and (2) distributed systems (monitoring). It may be necessary to distribute because the application itself is one of managing distributed resources (e.g., distributed sensor net) and communication delays preclude centralized processing, or it may be desirable to distribute because a single computational engine may not satisfy the needs of a given task. In addition, considerations of reliability may dictate distribution. Examples of multi-process language environment are given.

  11. Problem solving and decisionmaking: An integration

    NASA Technical Reports Server (NTRS)

    Dieterly, D. L.

    1980-01-01

    An attempt was made to redress a critical fault of decisionmaking and problem solving research-a lack of a standard method to classify problem or decision states or conditions. A basic model was identified and expanded to indicate a possible taxonomy of conditions which may be used in reviewing previous research or for systematically pursuing new research designs. A generalization of the basic conditions was then made to indicate that the conditions are essentially the same for both concepts, problem solving and decisionmaking.

  12. Problem solving with genetic algorithms and Splicer

    NASA Technical Reports Server (NTRS)

    Bayer, Steven E.; Wang, Lui

    1991-01-01

    Genetic algorithms are highly parallel, adaptive search procedures (i.e., problem-solving methods) loosely based on the processes of population genetics and Darwinian survival of the fittest. Genetic algorithms have proven useful in domains where other optimization techniques perform poorly. The main purpose of the paper is to discuss a NASA-sponsored software development project to develop a general-purpose tool for using genetic algorithms. The tool, called Splicer, can be used to solve a wide variety of optimization problems and is currently available from NASA and COSMIC. This discussion is preceded by an introduction to basic genetic algorithm concepts and a discussion of genetic algorithm applications.

  13. Partitioning SAT Instances for Distributed Solving

    NASA Astrophysics Data System (ADS)

    Hyvärinen, Antti E. J.; Junttila, Tommi; Niemelä, Ilkka

    In this paper we study the problem of solving hard propositional satisfiability problem (SAT) instances in a computing grid or cloud, where run times and communication between parallel running computations are limited.We study analytically an approach where the instance is partitioned iteratively into a tree of subproblems and each node in the tree is solved in parallel.We present new methods for constructing partitions which combine clause learning and lookahead. The methods are incorporated into the iterative approach and its performance is demonstrated with an extensive comparison against the best sequential solvers in the SAT competition 2009 as well as against two efficient parallel solvers.

  14. Solving coiled-coil protein structures

    DOE PAGESBeta

    Dauter, Zbigniew

    2015-02-26

    With the availability of more than 100,000 entries stored in the Protein Data Bank (PDB) that can be used as search models, molecular replacement (MR) is currently the most popular method of solving crystal structures of macromolecules. Significant methodological efforts have been directed in recent years towards making this approach more powerful and practical. This resulted in the creation of several computer programs, highly automated and user friendly, that are able to successfully solve many structures even by researchers who, although interested in structures of biomolecules, are not very experienced in crystallography.

  15. Assessing Affect after Mathematical Problem Solving Tasks: Validating the Chamberlin Affective Instrument for Mathematical Problem Solving

    ERIC Educational Resources Information Center

    Chamberlin, Scott A.; Powers, Robert A.

    2013-01-01

    The focus of the article is the validation of an instrument to assess gifted students' affect after mathematical problem solving tasks. Participants were 225 students identified by their district as gifted in grades four to six. The Chamberlin Affective Instrument for Mathematical Problem Solving was used to assess feelings, emotions, and…

  16. The Effect of Learning Environments Based on Problem Solving on Students' Achievements of Problem Solving

    ERIC Educational Resources Information Center

    Karatas, Ilhan; Baki, Adnan

    2013-01-01

    Problem solving is recognized as an important life skill involving a range of processes including analyzing, interpreting, reasoning, predicting, evaluating and reflecting. For that reason educating students as efficient problem solvers is an important role of mathematics education. Problem solving skill is the centre of mathematics curriculum.…

  17. Encouraging Sixth-Grade Students' Problem-Solving Performance by Teaching through Problem Solving

    ERIC Educational Resources Information Center

    Bostic, Jonathan D.; Pape, Stephen J.; Jacobbe, Tim

    2016-01-01

    This teaching experiment provided students with continuous engagement in a problem-solving based instructional approach during one mathematics unit. Three sections of sixth-grade mathematics were sampled from a school in Florida, U.S.A. and one section was randomly assigned to experience teaching through problem solving. Students' problem-solving…

  18. Students' Errors in Solving the Permutation and Combination Problems Based on Problem Solving Steps of Polya

    ERIC Educational Resources Information Center

    Sukoriyanto; Nusantara, Toto; Subanji; Chandra, Tjang Daniel

    2016-01-01

    This article was written based on the results of a study evaluating students' errors in problem solving of permutation and combination in terms of problem solving steps according to Polya. Twenty-five students were asked to do four problems related to permutation and combination. The research results showed that the students still did a mistake in…

  19. Preschoolers' Cooperative Problem Solving: Integrating Play and Problem Solving

    ERIC Educational Resources Information Center

    Ramani, Geetha B.; Brownell, Celia A.

    2014-01-01

    Cooperative problem solving with peers plays a central role in promoting children's cognitive and social development. This article reviews research on cooperative problem solving among preschool-age children in experimental settings and social play contexts. Studies suggest that cooperative interactions with peers in experimental settings are…

  20. Problem-Solving Models for Computer Literacy: Getting Smarter at Solving Problems. Student Lessons.

    ERIC Educational Resources Information Center

    Moursund, David

    This book is intended for use as a student guide. It is about human problem solving and provides information on how the mind works, placing a major emphasis on the role of computers as an aid in problem solving. The book is written with the underlying philosophy of discovery-based learning based on two premises: first, through the appropriate…

  1. Understanding Individual Problem-Solving Style: A Key to Learning and Applying Creative Problem Solving

    ERIC Educational Resources Information Center

    Treffinger, Donald J.; Selby, Edwin C.; Isaksen, Scott G.

    2008-01-01

    More than five decades of research and development have focused on making the Creative Problem Solving process and tools accessible across a wide range of ages and contexts. Recent evidence indicates that when individuals, in both school and corporate settings, understand their own style of problem solving, they are able to learn and apply process…

  2. Instruction Emphasizing Effort Improves Physics Problem Solving

    ERIC Educational Resources Information Center

    Li, Daoquan

    2012-01-01

    Effectively using strategies to solve complex problems is an important educational goal and is implicated in successful academic performance. However, people often do not spontaneously use the effective strategies unless they are motivated to do so. The present study was designed to test whether educating students about the importance of effort in…

  3. A SURVEY OF PROBLEM-SOLVING COURSES.

    ERIC Educational Resources Information Center

    EDWARDS, M.O.

    TO DETERMINE THE DESIRABILITY OF HAVING ITS OWN CREATIVITY COURSE, THE STANFORD RESEARCH INSTITUTE SURVEYED 43 ORGANIZATIONS ON THEIR PROBLEM SOLVING COURSES. FIFTEEN INDUSTRIAL CORPORATIONS, FOUR CONSULTING FIRMS, A GOVERNMENT AGENCY, A RESEARCH CORPORATION, AND NINE COLLEGES AND UNIVERSITIES RESPONDED WITH SPECIFIC INFORMATION ON COURSE LENGTH…

  4. Reflect ... for Better Problem Solving and Reasoning.

    ERIC Educational Resources Information Center

    Krulik, Stephen; Rudnick, Jesse A.

    1994-01-01

    Elaborates the final step of Polya's heuristic model, reflecting, to improve students' problem-solving performance. After checking answers for accuracy, the following steps are suggested: (1) test reasonableness and practicality; (2) write a summary paragraph; (3) find other solutions; (4) change the conditions; and (5) extend the problem. (MKR)

  5. Raise the Bar on Problem Solving

    ERIC Educational Resources Information Center

    Englard, Lisa

    2010-01-01

    In a 1981 diagnostic test, the Ministry of Education in Singapore found its country facing a challenge: Only 46 percent of students in grades 2-4 could solve word problems that were presented without such key words as "altogether" or "left." Yet today, according to results from the Trends in International Mathematics and Science Study (TIMSS…

  6. Problem Solving and Behavior Therapy Revisited

    ERIC Educational Resources Information Center

    Nezu, Arthur M.

    2004-01-01

    Over three decades ago, D'Zurilla and Goldfried (1971) published a seminal article delineating a model of problem-solving training geared to enhance social competence and decrease psychological distress. Since that time, a substantial amount of research has been conducted to test various hypotheses that this model has engendered. Much of this…

  7. Should Children Learn to Solve Problems?

    ERIC Educational Resources Information Center

    Watras, Joseph

    2011-01-01

    In this comparative essay, the author discusses the opposing educational theories of John Dewey and Gregory Bateson. While Dewey believed that the scientific method was the dominant method of solving problems and thereby acquiring knowledge that mattered, Bateson warned that this one-sided approach would lead to actions that could destroy the…

  8. Synthesizing Strategies Creatively: Solving Linear Equations

    ERIC Educational Resources Information Center

    Ponce, Gregorio A.; Tuba, Imre

    2015-01-01

    New strategies can ignite teachers' imagination to create new lessons or adapt lessons created by others. In this article, the authors present the experience of an algebra teacher and his students solving linear and literal equations and explain how the use of ideas found in past NCTM journals helped bring this lesson to life. The…

  9. Taylor series to solve friction problems

    NASA Astrophysics Data System (ADS)

    Béchet, Fabien; Lejeune, Arnaud; Potier-Ferry, Michel

    2010-06-01

    Thin metallic sheet transportation appears in numerous manufacturing processes such as continuous annealing, levelling or galvanization. It involves various nonlinear phenomena and, in particular, contact with friction. We develop a numerical method to solve this kind of mechanical problem, using shell finite elements and the Asymptotic Numerical Method (ANM). This article focuses on the treatment of the friction equations with ANM.

  10. Assessing Mathematical Problem Solving Using Comparative Judgement

    ERIC Educational Resources Information Center

    Jones, Ian; Swan, Malcolm; Pollitt, Alastair

    2015-01-01

    There is an increasing demand from employers and universities for school leavers to be able to apply their mathematical knowledge to problem solving in varied and unfamiliar contexts. These aspects are however neglected in most examinations of mathematics and, consequentially, in classroom teaching. One barrier to the inclusion of mathematical…