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1

Sports haematology.  

PubMed

While the crucial role of haemoglobin in aerobic exercise has been well accepted, there is still a great deal of controversy about the optimal haematological parameters in the athletic population. The initial part of this review will examine the question of anaemia in athletes. The most common finding in athletes is a dilutional pseudoanaemia that is caused by a plasma volume expansion, rather than an actual blood loss. It is not a pathological state and normalises with training cessation in 3 to 5 days. This entity should be distinguished from conditions associated with lowered blood counts, such as intravascular haemolysis or iron deficiency anaemia. The evaluation of true anaemia states in the athlete must take into account not only blood losses secondary to exercise, such as foot strike haemolysis or iron losses through sweat, but non-athletic causes as well. Depending on the age and sex of the athlete, consideration must be given to evaluation of the gastrointestinal or genitourinary systems for blood loss. Finally, a comprehensive nutritional history must be taken, as athletes, especially women, are frequently not consuming adequate dietary iron. The second section of the paper will deal with the very contentious issue of sickle cell trait. While there have been studies demonstrating an increased risk of sudden death in people with sickle cell trait, it is still quite rare and should not be used as a restriction to activity. Further, studies have demonstrated that patients with sickle cell trait have an exercise capacity that is probably normal or near normal. However, in the cases of sudden death, it has been secondary to rhabdomyolysis occurring among sickle cell trait athletes performing at intense exertion under hot conditions, soon after arriving at altitude. The recommendations are that athletes with sickle cell trait adhere to compliance with the general guidelines for fluid replacement and acclimatisation to hot conditions and altitude. The final section of the paper examines the issue of haematological manipulation for the purposes of ergogenic improvement. Although experiments with blood doping revealed improvements in running time to exhaustion and maximal oxygen uptake, the introduction of recombinant erythropoietin has rendered blood doping little more than a historical footnote. However, the improvements in performance are not without risk, and the use of exogenous erythropoietin has the potential for increased viscosity of the blood and thrombosis with potentially fatal results. Until a definitive test is developed for detection of exogenous erythropoietin, it will continue to be a part of elite athletics. PMID:10688281

Shaskey, D J; Green, G A

2000-01-01

2

Haematology and neurology  

Microsoft Academic Search

This review aims to update the reader on advances in the understanding of haematological conditions that may arise in neurological practice. Thrombophilia, antiphospholipid antibody syndrome, thrombotic thrombocytopenic purpura, sickle cell and clonal disorders associated with neuropathy are discussed.

Steven Austin; Hannah Cohen; Nick Losseff

2007-01-01

3

Liver in haematological disorders.  

PubMed

Prothrombotic haematological disorders, in particular myeloproliferative disorders, are identified in a significant proportion of patients with Budd-Chiari syndrome and portal vein thrombosis (PVT). Multiple prothrombotic disorders may coexist. PVT is diagnosed in one fourth of patients with cirrhosis and is more common with advanced liver disease and hepatocellular carcinoma. PVT in cirrhosis can precipitate decompensation. Intrahepatic microthrombosis may play a role in the pathogenesis of hepatic fibrosis. Sinusoidal obstruction syndrome is usually a complication of myeloablative treatment before haematopoietic stem cell transplantation. Post-transplant lymphoproliferative disorders can complicate liver transplantation and are related to Epstein-Barr virus infection. Hepatitis B reactivation in patients receiving chemotherapy for haematological malignancies is very common without pre-emptive treatment, and can lead to liver failure. Liver involvement is common in primary haematological diseases, such as haemolytic anaemias, lymphomas and leukaemia. PMID:24090939

Pieri, Giulia; Theocharidou, Eleni; Burroughs, Andrew K

2013-08-01

4

An international haematological survey  

PubMed Central

Haematological surveys of adult population samples were conducted simultaneously in 12 countries, all but one of which are in Europe. Haematological estimations on samples from nine of the countries were made in one central laboratory. Differences between countries in the mean levels of haemoglobin (and haematocrit and red cell count) were found to be relatively small, and the prevalence of levels below the arbitrary WHO levels for anaemia were, on the whole, low. In males, the evidence suggests a fall in haemoglobin level throughout adult life, which increases slightly in advanced age. In females, there is no evidence of any important relationship between age and haemoglobin level.

Elwood, P. C.; Hughes, J.; Abernethy, M.; Davies, R.; Gough, R.; Johnson, A. P.; Dubourg, A. Y.

1976-01-01

5

Malaria: a haematological disease.  

PubMed

Plasmodium falciparum malaria remains a major cause of mortality throughout the tropical world. Haematological abnormalities are considered a hallmark of malaria, bearing an impact on final outcome and representing indices of prognostic and follow-up value. These include severe anaemia, coagulation disturbances, leukocyte numerical or functional changes and spleen involvement. Anaemia involves red blood cell lysis due to parasite invasion, as well as mechanisms of intravascular haemolysis and decreased erythropoiesis. Exchange or blood transfusion is mainly recommended in the management of these patients. Haemorrhagic complications in severe malaria are relatively rare despite prominent thrombocytopenia and dysfunction in the coagulation pathway. Numerical, as well as functional changes in the white blood cell are less dramatic than other blood cell series, but still, remain a significant index of disease progression and ultimate prognosis. Finally, the role of the spleen in severe malaria is multifactorial. Care and vigilance should be taken against splenic rupture which is fatal and can occur despite appropriate antimalarial prophylaxis and treatment. PMID:22664049

Akinosoglou, Karolina S; Solomou, Elena E; Gogos, Charalambos A

2012-03-01

6

Haematological disorders in Down syndrome  

Microsoft Academic Search

Haematological abnormalities are common in children with Down syndrome. These are mostly benign. Neonatal changes include polycythaemia, neutrophilia and thrombocytopenia. In later childhood changes include secondary polycythaemia, red-cell macrocytosis, increased red-cell distribution width, leukopenia, and immune dysfunction. Transient myeloproliferative disorder occurs in about 5% and indicates a group at particularly high risk of subsequent leukaemia. A full blood count should

Rebecca James; Sally Kinsey

2009-01-01

7

HAEMATOLOGY OF THE WOOD DUCK, Chenonetta jubata  

Microsoft Academic Search

Haematologic values have been determined for the wood duck, Chenonetta jubata, captured in the western fringes of Sydney. Values for females were usually slightly higher, and this follows the pattern for other species sampled in the pre- nesting period. The differences in haematological values between males and females were not statistically significant.

R. C. MULLEY

1980-01-01

8

Growth factors in haematological cancers.  

PubMed

Since their discovery just under a century ago, growth factors (GFs) have been used almost ubiquitously in haematology. Many haematological cancers are associated with bone marrow failure, either as a direct consequence of the disease or its treatment. Colony stimulating factors (CSFs) have been used to address the problems associated with the resulting cytopenias, however, concerns about the potential leukaemogenic effects of some of these CSFs led to a degree of initial hesitancy in usage, particularly in the management of acute myeloid leukaemia (AML). This has now been largely overcome. Other limitations have included cost and side effect profiles (the latter particularly with the multilineage factors). There has been wide variation locally, nationally and internationally in the usage of GFs. The American Society of Clinical Oncologists (ASCO) attempted to rationalise the usage of GFs by producing a consensus document enumerating the evidence-based indications for use of GFs. There is little information on cost effectiveness, this remains an important issue for the future. Peripheral blood stem cell transplantation (PBSCT) has revolutionised the management of many malignant conditions and has contributed to the increased use of growth factors. Many other indications are emerging for GFs used singly or in combination. Current clinical applications of GFs include: i) amelioration of cytopenias following chemotherapy and stem cell transplantation, ii) chemotherapy dose maintenance and escalation, iii) chemosensitisation and modification of disease states, iv) optimisation of methods for mobilisation of progenitor stem cells, v) immunotherapy, and vi) as therapeutic targets for treatment of haematolgical malignancies. PMID:15989543

Osuji, Nnenna; Pettengell, R

2002-05-01

9

Haematology of Persian Fallow Deer ( Dama mesopotamica )  

Microsoft Academic Search

:   The haematology of Persian fallow deer was studied and the means of various parameters were determined for sex and age groups.\\u000a For total samples, the mean ± standard deviation of haematological parameters were: red blood cells (RBC), 7.42 ± 1.27 × 1012\\/l; haematocrit (PCV), 38.83 ± 7.38%; haemoglobin (Hb), 148.0 ± 17.3 g\\/l; mean cell volume (MCV), 50.84 ±

M. Mohri; M. R. Aslani; N. Shahbazian

2001-01-01

10

Haematological parameters of the otrich (Struthio camelus)  

Microsoft Academic Search

Haematological reference values according to age and sex, were obtained from healthy ostriches (Struthio camelus) held under intensive conditions. Erythrocytic parameters and indices demonstrated an age?related trend: the packed cell volume, haemoglobin concentration and erythrocyte count were lowest in ostriches of 1 to 3 months of age. The mean corpuscular volume increased with age until adulthood. No sex?linked differences were

A. Levi; B. Perelman; T. Waner; M. Van Grevenbroek; C. Van Creveld; R. Yagil

1989-01-01

11

Automated haematology analysis to diagnose malaria  

PubMed Central

For more than a decade, flow cytometry-based automated haematology analysers have been studied for malaria diagnosis. Although current haematology analysers are not specifically designed to detect malaria-related abnormalities, most studies have found sensitivities that comply with WHO malaria-diagnostic guidelines, i.e. ? 95% in samples with > 100 parasites/?l. Establishing a correct and early malaria diagnosis is a prerequisite for an adequate treatment and to minimizing adverse outcomes. Expert light microscopy remains the 'gold standard' for malaria diagnosis in most clinical settings. However, it requires an explicit request from clinicians and has variable accuracy. Malaria diagnosis with flow cytometry-based haematology analysers could become an important adjuvant diagnostic tool in the routine laboratory work-up of febrile patients in or returning from malaria-endemic regions. Haematology analysers so far studied for malaria diagnosis are the Cell-Dyn®, Coulter® GEN·S and LH 750, and the Sysmex XE-2100® analysers. For Cell-Dyn analysers, abnormal depolarization events mainly in the lobularity/granularity and other scatter-plots, and various reticulocyte abnormalities have shown overall sensitivities and specificities of 49% to 97% and 61% to 100%, respectively. For the Coulter analysers, a 'malaria factor' using the monocyte and lymphocyte size standard deviations obtained by impedance detection has shown overall sensitivities and specificities of 82% to 98% and 72% to 94%, respectively. For the XE-2100, abnormal patterns in the DIFF, WBC/BASO, and RET-EXT scatter-plots, and pseudoeosinophilia and other abnormal haematological variables have been described, and multivariate diagnostic models have been designed with overall sensitivities and specificities of 86% to 97% and 81% to 98%, respectively. The accuracy for malaria diagnosis may vary according to species, parasite load, immunity and clinical context where the method is applied. Future developments in new haematology analysers such as considerably simplified, robust and inexpensive devices for malaria detection fitted with an automatically generated alert could improve the detection capacity of these instruments and potentially expand their clinical utility in malaria diagnosis.

2010-01-01

12

Automated haematology analysis to diagnose malaria.  

PubMed

For more than a decade, flow cytometry-based automated haematology analysers have been studied for malaria diagnosis. Although current haematology analysers are not specifically designed to detect malaria-related abnormalities, most studies have found sensitivities that comply with WHO malaria-diagnostic guidelines, i.e. ? 95% in samples with > 100 parasites/?l. Establishing a correct and early malaria diagnosis is a prerequisite for an adequate treatment and to minimizing adverse outcomes. Expert light microscopy remains the 'gold standard' for malaria diagnosis in most clinical settings. However, it requires an explicit request from clinicians and has variable accuracy. Malaria diagnosis with flow cytometry-based haematology analysers could become an important adjuvant diagnostic tool in the routine laboratory work-up of febrile patients in or returning from malaria-endemic regions. Haematology analysers so far studied for malaria diagnosis are the Cell-Dyn®, Coulter® GEN·S and LH 750, and the Sysmex XE-2100® analysers. For Cell-Dyn analysers, abnormal depolarization events mainly in the lobularity/granularity and other scatter-plots, and various reticulocyte abnormalities have shown overall sensitivities and specificities of 49% to 97% and 61% to 100%, respectively. For the Coulter analysers, a 'malaria factor' using the monocyte and lymphocyte size standard deviations obtained by impedance detection has shown overall sensitivities and specificities of 82% to 98% and 72% to 94%, respectively. For the XE-2100, abnormal patterns in the DIFF, WBC/BASO, and RET-EXT scatter-plots, and pseudoeosinophilia and other abnormal haematological variables have been described, and multivariate diagnostic models have been designed with overall sensitivities and specificities of 86% to 97% and 81% to 98%, respectively. The accuracy for malaria diagnosis may vary according to species, parasite load, immunity and clinical context where the method is applied. Future developments in new haematology analysers such as considerably simplified, robust and inexpensive devices for malaria detection fitted with an automatically generated alert could improve the detection capacity of these instruments and potentially expand their clinical utility in malaria diagnosis. PMID:21118557

Campuzano-Zuluaga, Germán; Hänscheid, Thomas; Grobusch, Martin P

2010-11-30

13

Haematological and morphological responses of broiler chicks to hypoxia  

Microsoft Academic Search

Broiler chicks were subjected to experimentally?induced hypoxia and the haematology, together with the histopathology and ultrastructure of heart, liver, lung and kidney and the ultracytochemistry of heart tissues were examined. The haemoglobin concentration, packed cell volume and red blood cell counts were significantly increased compared with controls. The results resembled the haematology of similar aged broilers with an ascitic syndrome

M. H. Maxwell; S. Spence; G. W. Robertson; M. A. Mitchell

1990-01-01

14

Use of Antibiotics in a Haematology Ward - An Audit  

Microsoft Academic Search

Introduction: Rising rates of antibiotic resistance prompted a review of antibiotic use policies hospitalwide. The Department of Haematology established a new set of consensus guidelines in 2002 for antibiotic use in febrile neutropenia. The aim of our study was to audit adherence to the guidelines established for febrile neutropenia in patients treated for haematologic malignancies. Materials and Methods: An antibiotic

Gee Chuan Wong; Ban Hock Tan; Wong Gee Chuan

2008-01-01

15

Interferon-alpha in childhood haematological malignancies.  

PubMed Central

The application of cytostatics has brought about a breakthrough in the treatment of childhood haematological malignancies in the past 20 years. Chemotherapy appears to be least successful in the rare, low and very high mitotic index diseases, which often have an enormous tumour-burden. The suitability of chemotherapy in minimal residual leukaemia is also of some doubt. In these situations a 'conservative' treatment may be more appropriate. Because interferon-alpha has a distinct mechanism of action, and a broad-spectrum haematopoietic inhibitory activity, it is relatively nontoxic and noncancerogenic, and it may have a role in the treatment of malignant haematological disorders, either as a mono- or combination therapy. The exact indications and dosages for interferon in childhood malignancies are far from clear. Up to now, it has proved to be most efficacious in small tumour masses, providing a theoretical basis for application in minimal residual disease. Controlled clinical data, however, are not yet available. It remains to be determined whether or not interferon can be added to current chemotherapy protocols without a significant reduction of dose. Hopefully, a deeper understanding of the activities of interferon will allow us to plan better trials with combination treatments.

Simko, R.; Nagy, K.

1996-01-01

16

Spleen in haematological malignancies: spectrum of imaging findings  

PubMed Central

Imaging morphology and metabolic activity of splenic lesions is of paramount importance in patients with haematological malignancies; it can alter tumour staging, treatment protocols and overall prognosis. CT, MRI and positron emission tomography (PET)/CT have been shown to be powerful tools for the non-invasive assessment of splenic involvement in various haematological malignancies. Since many haematological malignancies and non-neoplastic conditions can involve the spleen and imaging manifestations can overlap, imaging and clinical findings outside of the spleen should be looked for to narrow the differential diagnosis; confirmation can be obtained by pathological findings. Radiologists should be familiar with the cross-sectional imaging patterns of haematological malignancies involving the spleen as well as non-neoplastic splenic findings common in these patients to facilitate their care and follow-up. This pictorial review provides the common and uncommon imaging appearances and complications of various haematological malignancies involving the spleen on CT, MRI and PET/CT, and common pitfalls in diagnosis.

Saboo, S S; Krajewski, K M; O'Regan, K N; Giardino, A; Brown, J R; Ramaiya, N; Jagannathan, J P

2012-01-01

17

Zygomycosis in Immunocompromised non-Haematological Patients.  

PubMed

Zygomycoses caused by fungi of the mucorales order (mucormycoses) are emerging fungal diseases with a high fatality rate. The most important risk factors include neutropenia or functional neutropenia, diabetic ketoacidosis, iron overload, major trauma, prolonged use of corticosteroids, illicit intravenous drug (ID) use, neonatal prematurity, malnourishment, and maybe a previous exposure to antifungal agents with no activity against zygomycetes, such as voriconazole and echinocandins.A high index of suspicion is crucial for the diagnosis, as prompt and appropriate management can considerably reduce morbidity and mortality. Suspicion index can be increased through recognition of the differential patterns of clinical presentation. In the non- haematological immunocompromised patients, mucormycosis can manifest in various clinical forms, depending on the underlying condition: mostly as rhino-orbital or rhino-cerebral in diabetes patients, pulmonary infection in patients with malignancy or solid organ transplantation, disseminated infection in iron overloaded or deferoxamine treated patients, cerebral - with no sinus involvement - in ID users, gastrointestinal in premature infants or malnourishment, and cutaneous after direct inoculation in immunocompetent individuals with trauma or burns.Treating a patient's underlying medical condition and reducing immunosuppression are essential to therapy. Rapid correction of metabolic abnormalities is mandatory in cases such as uncontrolled diabetes, and corticosteroids or other immunosuppressive drugs should be discontinued where feasible. AmphotericinB or its newer and less toxic lipid formulations are the drugs of choice regarding antifungal chemotherapy, while extensive surgical debridement is essential to reduce infected and necrotic tissue. A high number of cases could be prevented through measures including diabetes control programmes and proper pre- and post-surgical hygiene. PMID:21625316

Petrikkos, George; Drogari-Apiranthitou, Miranda

2011-03-15

18

Normal and clinical haematology of captive cranes (gruiformes)  

Microsoft Academic Search

Fall blood counts on 56 clinically normal cranes of nine different species have provided reference values for the interpretation of haematological changes in 13 cranes presenting with abnormal clinical signs. Hypochromic anaemia, heterophilia and lymphocytosis were found in birds with probable Mycobacterium avium infection and heterophilia and lymphocytosis in birds with bumblefoot, arthritis, nephrosis and cardiac myo?pathy. In several of

Christine Hawkey; J. H. Samour; D. G. Ashton; M. G. Hart; R. N. Cindery; J. M. Ffinch; D. M. Jones

1983-01-01

19

Haematological and biochemical parameters during pregnancy and lactation in sows  

Microsoft Academic Search

The aim of this research was to describe changes in serum concentrations of some micronutrients, and haematological parameters during pregnancy and lactation of sows. Data presented here were obtained by using blood samples from healthy, conventionally managed sows from a breeding herd. The samples were taken at three different points in the physiological production process: I. from the 30th to

Vladimir Mrljak; Jelena Pompe Gotal

20

EFFECT OF CYPERMETHRIN ON CLINICO-HAEMATOLOGICAL PARAMETERS IN RABBITS  

Microsoft Academic Search

The aim of this study was to know the clinical and haematological changes in female rabbits following the treatment with cypermethrin (CY). In this study, 24 female rabbits were divided randomly into four equal groups A, B, C and D. Groups A, B and C were injected intraperitoneally CY @ 25, 50 and 75 mg\\/Kg b. wt., respectively, while the

M. KAMAL SHAH; A. KHAN; F. RIZVI; M. SIDDIQUE

21

Variation in haematological parameters among inbred strains of rat  

Microsoft Academic Search

Summary Haematological determinations were carried out on 7 inbred strains of rats using a standardized procedure. Significant genetic variation was found for many of the parameters although none of the strains had values which could be termed pathological. Measurements also varied significantly from day to day. Factorial analysis of variance is shown to be a powerful method for the investigation

D. P. Lovell; R. K. Archer; Joan Riley; R. K. Morgan

1981-01-01

22

The Potential Role of Aurora Kinase Inhibitors in Haematological Malignancies  

PubMed Central

Summary Aurora kinases play an important role in the control of the cell cycle and have been implicated in tumourigenesis in a number of cancers. Among the haematological malignancies, overexpression of Aurora kinases has been reported in acute myeloid leukaemia, chronic myeloid leukaemia, acute lymphoblastic leukaemia, multiple myeloma, aggressive non-Hodgkin lymphoma and Hodgkin lymphoma. A large number of Aurora kinase inhibitors are currently in different stages of clinical development. In addition to varying in their selectivity for the different Aurora kinases, some also have activity directed at other cellular kinases involved in important molecular pathways in cancer cells. This review summarizes the biology of Aurora kinases and discusses why they may be good therapeutic targets in different haematological cancers. We describe preclinical data that has served as the rationale for investigating Aurora kinase inhibitors in different haematological malignancies, and summarize published results from early phase clinical trials. While the anti-tumour effects of Aurora kinase inhibitors appear promising, we highlight important issues for future clinical research and suggest that the optimal use of these inhibitors is likely to be in combination with cytotoxic agents already in use for the treatment of various haematological cancers.

Farag, Sherif S.

2011-01-01

23

Haematology of clinically normal and abnormal captive llamas and guanacoes  

Microsoft Academic Search

Blood counts of healthy juvenile and adult llamas (Lama glama) and guanacoes (L guanacoe) showed that guanacoes have higher red cell counts, haemoglobin values and packed cell volumes than llamas. In both species, the numbers of lymphocytes and platelets were higher in juveniles than in adults. By reference to the values found in normal animals, abnormal haematological variations were detected

CM Hawkey; FM Gulland

1988-01-01

24

Haematological studies of Emblica officinalis formulation on Wistar Rats  

Microsoft Academic Search

Over three-quarters of the world population relies mainly on plants and plant extracts for health care. More than 30% of the entire plant species were used for medicinal purposes. Emblica officinalis is one of the widely used food supplement for the prevention of many ailments. A comprehensive account of the effect of Emblica officinalis formulation on some of the haematological

Narendra Kumar Savala; Parvathaneni Nagasri Haritha; Mangamoori Lakshmi Narasu

2012-01-01

25

Is intensive care justified for patients with haematological malignancies?  

Microsoft Academic Search

The course of 260 adults with haematological malignancies admitted to a medical intensive care unit was studied to evaluate the value of life support techniques and to research predictive factors. The overall in the medical intensive care unit (MICU) and hospital mortality rates were respectively 43% (113 patients) and 57% (148 patients). Among survivors, 64% (49 patients) were still alive

F. Brunet; J. J. Lanore; J. F. Dhainaut; F. Dreyfus; J. F. Vaxelaire; S. Nouira; T. Giraud; A. Armaganidis; J. F. Monsallier

1990-01-01

26

Advances in Haematological Pharmacotherapy in 21st Century  

PubMed Central

Last quarter of twentieth century and the first 10 years of 21st century has seen phenomenal development in haematological pharmacotherapy. Tailor made chemotherapeutic agents, vast array of monoclonal antibodies, epigenetic modifiers, growth factors for red cells white cells and platelets, peptidomimetics as growth factors, newer thrombin inhibitors, safer plasma derived protein molecules, recombinant molecules, newer immunomodulators, enzyme replacement therapy and above all a plethora of targetted molecules targeting innumerable pathways involved in cell division, growth, proliferation and apoptosis has given immense number of clinically usable molecules in the hand of modern haematologists to treat diverse hitherto untreatable haematological disorders effectively. In addition many old molecules are finding newer uses in diverse fields, thalidomide as an antiangiogenic molecule is a prime example of this genre. Present overview has tried to capture this rapidly evolving area in a broad canvas without going into details of indications and contraindications of the use of various drugs.

Ghosh, Kinjalka

2010-01-01

27

Moral distress in nurses in oncology and haematology units.  

PubMed

One of the difficulties nurses experience in clinical practice in relation to ethical issues in connection with young oncology patients is moral distress. In this descriptive correlational study, the Moral Distress Scale-Paediatric Version (MDS-PV) was translated from the original language and tested on a conventional sample of nurses working in paediatric oncology and haematology wards, in six north paediatric hospitals of Italy. 13.7% of the total respondents claimed that they had changed unit or hospital due to moral distress. The items with the highest mean intensity in the sample were almost all connected with medical and nursing competence and have considerably higher values than frequency. The instrument was found to be reliable. The results confirmed the validity of the MDS-PV (Cronbach's alpha = 0.959). This study represents the first small-scale attempt to validate MDS-PV for use in paediatric oncology-haematology nurses in Italy. PMID:22457383

Lazzarin, Michela; Biondi, Andrea; Di Mauro, Stefania

2012-03-01

28

Haematological parameters as bioindicators of insecticide exposure in teleosts  

Microsoft Academic Search

Haematological parameters, such as erythrocyte and leucocyte count, erythrocyte indices and thrombocyte number vis-a-vis coagulation\\u000a of blood has been considered bioindicators of toxicosis in fish following exposure to organochlorine, organophosphate, carbamate\\u000a and pyrethroid insecticides. This review deals with the effects of insecticides on the morphology of red blood cells, total\\u000a erythrocyte count, haemoglobin content, haematocrit, mean corpuscular volume, mean corpuscular

Narendra Nath Singh; Anil Kumar Srivastava

2010-01-01

29

Haematological changes of cats with Toxoplasma gondii -specific antibodies  

Microsoft Academic Search

The purpose of this study is to access the usefulness of haematological parameters in diagnosing of chronic toxoplasmosis\\u000a in cats. Antibodies of Toxoplasma gondii were detected in 50 cats using an enzyme-linked immunosorbent assay. Immunoglobulin M (IgM) and immunoglobulin G (IgG) were\\u000a measured with the cutoff point for T. gondii considered to be 1:64. Fifteen (30%) of the cats were

S. Javadi; S. Asri Rezaei; H. Tajik; M. Hadian; F. Shokouhi

2010-01-01

30

Modern molecular diagnostics and the management of haematological malignancies.  

PubMed

The advent of molecular biological techniques has led to a radical improvement in the evaluation of haematological disorders and offers exciting future prospects. In particular it has led to the improved recognition of distinct clinicopathological entities defined by a combination of morphological, immunophenotypic and chromosomal features. Antibody-based techniques, namely immunophenotyping and immunocytochemical techniques, are essential to malignant haematological diagnosis. More novel is the diagnostic use of antibodies against novel fusion proteins, for example in AML, M3 and anaplastic lymphoma. PCR-based techniques allow the identification of genetic mutations and translocations including such common translocations as t(14;18), t(9;22), inv 16 and t(8;21). Fish-based techniques are greatly improving the ability to detect genetic abnormalities including those conditions with low cell proliferation and current research-based techniques include the combination of FISH with cell surface markers (FICTION), FIBRE FISH and Comparative Genomic Hybridization. Molecular techniques are essential in monitoring residual disease which is best illustrated in CML. The development of real-time automated PCR offers exciting prospects in this field. The increasing number of tests, the need for an integrated approach to diagnosis and the need for cost effectiveness indicate that such services should be provided by a specialized haematology laboratory. PMID:9681227

Morgan, G J; Pratt, G

1998-06-01

31

Atlas of Genetics and Cytogenetics in Oncology and Haematology  

NSDL National Science Digital Library

The Atlas of Genetics and Cytogenetics in Oncology and Haematology, a peer-reviewed online journal and database provided by Infobiogen, is "devoted to genes, cytogenetics, and clinical entities in cancer, and cancer-prone diseases." Users can search the materials by genes, leukaemias, solid tumors, cancer prone diseases, and chromosomes. Researchers can find links to scientific societies and meetings. Students can explore educational materials on Mendelian and non-Mendelian Inheritance, chromosomes, population genetics, and additional human genetics topics. The website also offers reviews and case reports.

32

Invented fairy tales in groups with onco-haematological children.  

PubMed

BACKGROUND: The impact of an onco-haematological illness for children is a traumatic event that opens to pain, hospitalizations and interrupts the continuity of daily life. It is difficult for the child to make meaning, to share the pain or ask a question related to the illness because, often, the parents or doctors cannot find a way to communicate in a suitable way for the child who remains in a situation of 'unspoken', where, fear, anxiety and pain cannot find a space to express. METHODS: The present research-intervention uses the methodology of invented fairy tales in groups with onco-haematological children, in the hospital, in order to explore the organization of the meanings at the base of the tales co-constructed by the participants underlying weaknesses and strengths of the invented fairy tales in groups intervention. The invented fairy tales in groups is used as a tool, such as a play, to express, share and support the experience of the illness of children. Forty-nine children participated to the invented fairy tales in groups in an onco-haematological hospital. Within a quali-quantitative framework we performed a thematic analysis of elementary context, cluster analysis, on the fairy tales considered as a unique narrative corpus of the thought of the group. RESULTS: The analysis shows four thematic clusters: fantasy as search for a meaning, 29.71%, the group as a space for illusions, 27.90%, the illness as a family problem, 25.72%, anchoring reality, 16.67%. The results highlighted three main carriers of sense: the representation of illness/the relational world/the representation of the institution. CONCLUSIONS: The use of invented-fairy-tales groups allowed the onco-haematological children to tell and share the experience of illness through a different way, which let them express symbolically their pain. The invented fairy tale in groups becomes a mediator of psychic processes which offer new solutions while improving interpersonal relationships/communication between the participants in group. PMID:23672257

Margherita, G; Martino, M L; Recano, F; Camera, F

2013-05-14

33

Effect of long-term highly active antiretroviral therapy (HAART) on some haematological parameters of HIV-infected Nigerians  

Microsoft Academic Search

Haematologic abnormalities are among the most common manifestations of advanced HIV infection and AIDS. The aim of this case controlled study was to examine the long-term effect of HAART on the haematological profile of HIV-infected Nigerians. One hundred consecutive patients were randomized to take a fixed drug combination of stavudine, lamivudine and nevirapine. Haematological parameters were analyzed at baseline and

Osaro Erhabor; Emmanuel Kufre Uko; Osekhuiemen Adebayo Ejele; Confidence Wachukwu

34

Follow up study of haematological effects in workers exposed to 2-methoxyethanol  

PubMed Central

Aims: To examine the association between 2-methoxyethanol (2-ME) exposure and haematological effects, as well as the recovery from these haematological effects with continuous reduction in exposure to 2-ME. Methods: Twenty nine exposed and 90 non-exposed workers were recruited. Haematological parameters, eight hour full shift personal exposure to 2-ME, and urinary 2-methoxyacetic acid (MAA) were repeatedly measured in three consecutive surveys within six months. Results: Results of haematological examination in the first exposure survey showed that haemoglobin, packed cell volume, and red blood cell count in the male exposed workers were significantly lower than those in the comparison workers. The frequency of anaemia in the exposed group (42%) was significantly higher than that in the comparison group (3%). The haematological effects were significantly associated with the urinary MAA of exposed workers. The haematological effects had returned to normal in the first follow up survey 2.5 months later, when a reduction in 2-ME exposure was noted. Haematological results of the second follow up examination six months later remained normal. The mean airborne exposure of 2-ME in the three surveys dropped from 35.7 to 2.65, then to 0.55 ppm. The mean urinary MAA of exposed workers in the three surveys was reduced from 57.7 to 24.6, then to 13.5 mg/g creatinine (n = 29). The reduction in exposure through both inhalation and potential dermal contact with 2-ME might account for the haematological recovery. Conclusion: 2-ME is a haematological toxin which leads to anaemia in exposed workers. However, the toxic haematological effects of 2-ME persist for only a short period of time after cessation or reduction of exposure.

Shih, T; Hsieh, A; Chen, Y; Liao, G; Chen, C; Chou, J; Liou, S

2003-01-01

35

Haemodynamic and haematologic effects of Acanthaster planci venom in dogs.  

PubMed

This study was designed to examine haemodynamic and haematologic effects of the crown-of-thorns starfish venom (Acanthaster planci venom: APV) in dogs. Severe systemic hypotension, thrombocytopenia and leukopenia were induced by APV (1.0 mg protein/kg i.v.), followed by gradual return to the baseline level within 60 min. Hypotension was presumably caused by two factors: an early decrease in systemic vascular resistance and the large reduction in cardiac output due to reduced ventricular filling. Indomethacin, a cyclooxygenase inhibitor, remarkably suppressed systemic hypotension induced by APV. The peak reduction in systemic pressure was associated with concomitant rise of plasma 6-keto-PGF1 alpha, a major stable metabolite of prostacyclin. Thus, the hypotensive effect of APV may be caused primarily by prostacyclin and/or some vasodilating prostaglandins. In contrast, thrombocytopenia and leukopenia were not affected by cyclooxygenase inhibitor, 5-lipoxygenase inhibitor or platelet activating factor (PAF) receptor antagonist. When APV was administered repeatedly, tachyphylaxis was developed in haemodynamic effects, but not in haematologic effects. These findings suggest that APV-induced hypotensive effects may occur mainly through endogenous production of vasodilating prostaglandins including prostacyclin, although APV-induced thrombocytopenia and leukopenia may be caused by other mechanism(s) unrelated to arachidonate metabolites and/or PAF. PMID:7846692

Shiroma, N; Noguchi, K; Matsuzaki, T; Ojiri, Y; Hirayama, K; Sakanashi, M

1994-10-01

36

Intestinal helminths induce haematological changes in dogs from Jabalpur, India.  

PubMed

The effect of canine intestinal helminths on the haematological profile of 200 dogs, of both sexes and variable age, visiting university veterinary clinics for routine examination was investigated. The dogs were assigned to parasitized (n = 39) and non-parasitized (n = 161) groups of animals. Coprological examination revealed a 19.5% prevalence of different species of the helminths. Of these animals, 10.25% had mixed infections with Ancylostoma caninum, Toxascaris spp. and Dipylidium caninum. The intensity of A. caninum infection was the highest, with mean egg counts of 951.43 (standard error 88.66), followed by Toxascaris 283.33 (standard error 116.81) and D. caninum. The parasitized animals had significantly lower levels of haemoglobin, packed cell volume and total erythrocyte counts than non-parasitized animals (P < 0.01). Values of other parameters, except for lymphocytes and eosinophils, were not different between the two groups. Analyses of the haematological profile revealed normocytic hypochromic anaemia in the parasitized group of animals. PMID:21110909

Qadir, S; Dixit, A K; Dixit, P; Sharma, R L

2010-11-29

37

Long Non-Coding RNAs in Haematological Malignancies  

PubMed Central

Long non-coding RNAs (lncRNAs) are functional RNAs longer than 200 nucleotides in length. LncRNAs are as diverse as mRNAs and they normally share the same biosynthetic machinery based on RNA polymerase II, splicing and polyadenylation. However, lncRNAs have low coding potential. Compared to mRNAs, lncRNAs are preferentially nuclear, more tissue specific and expressed at lower levels. Most of the lncRNAs described to date modulate the expression of specific genes by guiding chromatin remodelling factors; inducing chromosomal loopings; affecting transcription, splicing, translation or mRNA stability; or serving as scaffolds for the organization of cellular structures. They can function in cis, cotranscriptionally, or in trans, acting as decoys, scaffolds or guides. These functions seem essential to allow cell differentiation and growth. In fact, many lncRNAs have been shown to exert oncogenic or tumor suppressor properties in several cancers including haematological malignancies. In this review, we summarize what is known about lncRNAs, the mechanisms for their regulation in cancer and their role in leukemogenesis, lymphomagenesis and hematopoiesis. Furthermore, we discuss the potential of lncRNAs in diagnosis, prognosis and therapy in cancer, with special attention to haematological malignancies.

Garitano-Trojaola, Andoni; Agirre, Xabier; Prosper, Felipe; Fortes, Puri

2013-01-01

38

[The spleen in non-malignant haematological disorders].  

PubMed

The spleen functions as a filter of the circulating blood, removing aging or abnormal red blood cells, intraerythrocyte inclusions as well as foreign particals. As the spleen is composed of lymphocytic tissue, circulatory elements and mononuclear phagocytic cells it plays an important role in the nonspecific as well as the specific immune response. Additionally, the spleen serves as a reservoir for circulating blood cells, especially platelet sequestration by the spleen is well do cumented. The spleen produces blood cells during fetal development and in certain haematological disorders such as myelofibrosis. The destruction of red blood cells within the splenic cords releases iron in the circulation, which is recycled and used to manufacture new erythrocytes in the bone marrow. In several non-malignant haematological disorders antibody-coated cells are cleared from the circulation by phagocytic cells of the spleen. This involves erythrocytes in autoimmunhaemolytic anaemias, platelets in immunthrombocytopenia and neutrophils in Felty syndrome. In hereditary spherocytosis the spleen destroys the resulting defective, spherical red cells. In pyruvate kinase deficiency impaired production of adenosine triphosphate leads to destruction of red blood cells in the spleen or in the liver. In sickle cell anaemia the defective erythrocytes cause sludging and thrombosis in small vessels with infarcts for instance in the spleen, which over time can result in autosplenectomy. In thalassaemia major abnormal haemoglobin forms protein precipitates in the red cells with development of a severe hypochromic anaemia with haemolysis and intramedullary inef fective erythropoiesis. Therapeutic splenectomy can be an option in all of these mentioned non-malignant haematological disorders. The rationale and the pathophysiology of its role in thrombotic thrombocytopenic purpura is probably at least well understood. The use of new and effective drugs such as the monoclonal antibody rituximab or thrombopoietin agonists in immunthrombocytopenia as well as the option of a laparoscopic rather than an open splenectomy have changed our view on benefits and risks of splenectomy in recent years. To minimize these risks such as overwhelming postsplenectomy infection or abdominal vein thrombosis, prophylactic vaccination, postoperative thromboembolism prophylaxis and patient education is mandatory. PMID:23454562

Rüfer, Axel; Wuillemin, Walter A

2013-03-01

39

Oncogenic properties of sphingosine kinases in haematological malignancies.  

PubMed

The sphingosine kinases (SphKs) have relatively recently been implicated in contributing to malignant cellular processes with particular interest in the oncogenic properties of SPHK1. Whilst SPHK1 has received considerable attention as a putative oncoprotein, SPHK2 has been much more difficult to study, with often conflicting data surrounding its role in cancer. Initial studies focused on non-haemopoietic malignancies, however a growing body of literature on the role of sphingolipid metabolism in haemopoietic malignancies is now emerging. This review provides an overview of the current state of knowledge of the SphKs and the bioactive lipid sphingosine 1-phosphate (S1P), the product of the reaction they catalyse. It then reviews the current literature regarding the roles of S1P and the SphKs in haemopoietic malignancies and discusses the compounds currently available that modulate sphingolipid metabolism and their potential and shortcomings as therapeutic agents for the treatment of haematological malignancies. PMID:23521541

Wallington-Beddoe, Craig T; Bradstock, Kenneth F; Bendall, Linda J

2013-03-25

40

Targeting Metabolism and Autophagy in the Context of Haematologic Malignancies  

PubMed Central

Autophagy is a cellular process that maintains the homeostasis of the normal cell. It not only allows for cell survival in times of metabolic stress with nutrient recycling but also is able to lead to cell death when required. During malignant transformation the cell is able to proliferate and survive. This is due to altered cell metabolism and the presence of altered genetic changes that maintain the cell survival. Metabolism was considered an innocent bystander that was a consequence of the increased nutrient requirement for the survival and proliferation of haematological malignancies. The interdependency of metabolism and cellular mechanisms such as autophagy are becoming more evident and important. This interdependence contributes to increased cancer progression and drug resistance. In this paper we aim to discuss autophagy, how it pertains to metabolism in the context of hematologic malignancies, and the implications for therapy.

Banerji, Versha; Gibson, Spencer B.

2012-01-01

41

Haematological and haemorheological changes associated with cigarette smoking.  

PubMed Central

A prospective study was undertaken to establish the haematological and haemorheological changes associated with long standing cigarette smoking in 20 heavy smokers and to assess whether any such changes were reversible after smoking was stopped. Highly significant differences were observed in whole blood viscosity, plasma viscosity, plasma fibrinogen concentrations, packed cell volume, and carboxyhaemoglobin concentrations between smokers and non-smokers. Ten of the subjects were followed up for two weeks after stopping smoking by which time whole blood viscosity and carboxyhaemoglobin concentrations had improved significantly and indications of improvement could be seen in all other measurements. Two of these subjects were further followed up for two months when all the measured variables were comparable with those in non-smokers. No correlation could be established between carboxyhaemoglobin concentration and any of the other variables. Although patients' compliance may be difficult to obtain, further prospective studies would be required to confirm our findings.

Galea, G; Davidson, R J

1985-01-01

42

CLINICAL AND HAEMATOLOGICAL STUDIES IN DOGS, EXPERIMENTALLY INFECTED WITH TRICHURIS VULPIS  

Microsoft Academic Search

Summary Kirkova, Z., P. Petkov & D. Goundasheva, 2005. Clinical and haematological studies in dogs, experimentally infected with Trichuris vulpis. Bulg. J. Vet. Med., 8, No 2, 141?148. The experiment was performed on 9 mixed-breed dogs (6 infected and 3 non-infected controls). The experimental infection was provoked with Trichuris vulpis (10000 eggs\\/kg). The clinical signs and the following haematological parameters

Z. KIRKOVA; P. PETKOV; D. GOUNDASHEVA

2005-01-01

43

Haematological health assessment in a passerine with extremely high proportion of basophils in peripheral blood  

Microsoft Academic Search

Haematological methods are widely utilised among avian ecologists as a means for individual health assessment. However, the\\u000a technical simplicity of some of the tests may easily lead to oversimplification of the evaluation. Here, we show in the Scarlet\\u000a Rosefinch (Carpodacus erythrinus) that haematological parameters other than the widely used heterophil\\/lymphocyte (H\\/L) ratio may be important to investigate.\\u000a We give the

Michal Vinkler; Jan Schnitzer; Pavel Munclinger; Jan Votýpka; Tomáš Albrecht

2010-01-01

44

Effect of altitude training on haematological variables in women volleyball players  

Microsoft Academic Search

BackgroundAthletes from many endurance disciplines include altitude training in their yearly training programme. In volleyball, it is necessary to clarify the haematological benefits and improvement in sports performance.ObjectiveTo investigate the influence of moderate altitude on haematological variables in a women's volleyball team (Honor Division – Spain).Design and settingWe assessed 14 elite female volleyball players for two weeks in normoxia (640

B Cueto-Martín; J C de la Cruz-Márquez; J C de la Cruz-Campos; A de la Cruz-Campos

2011-01-01

45

Thrombopoietin-receptor agonists in haematological disorders: the Danish experience.  

PubMed

The objective of this study was to investigate the use of thrombopoietin-receptor agonists (TPO-ra) in patients with refractory primary immune thrombocytopenia (ITP) as well as off-label use of TPO-ra in Danish haematology departments. Hospital medical records from 32 of the 39 patients having received TPO-ra from 2009 to 1 May 2011 were available for data collection and included in the study. Of these patients, 15 received TPO-ra for refractory primary ITP, 7 for secondary ITP (chronic lymphatic leukaemia, systemic lupus erythematosus, Evans syndrome, human immunodeficiency virus and celiac disease) and 10 were treated for non-ITP (chemotherapy-induced, acute myeloid leukaemia, myelodysplastic syndrome, hereditary spherocytosis and suspected chemically induced thrombocytopenia). Initial response to TPO-ra defined as platelet counts >30 × 10(9)/l after 4 weeks of treatment was found in 59% of primary ITP patients, 57% of patients with secondary ITP and 40% of patients with non-ITP. There were four deaths in the cohort, three of which were related to pre-existing medical conditions. Otherwise adverse effects were in general mild. This Danish retrospective registration study has demonstrated that in the off-protocol setting, the use of TPO-ra is associated with response rates largely similar to those seen in previous protocol-monitored studies and no new adverse events were reported. PMID:22185370

Gudbrandsdottir, Sif; Frederiksen, Henrik; Hasselbalch, Hans

2011-12-20

46

New category of probable invasive pulmonary aspergillosis in haematological patients.  

PubMed

The European Organization for Research and Treatment of Cancer and the Mycosis Study Group (EORTC-MSG) radiological definitions of invasive pulmonary aspergillosis (IPA) may lack diagnostic sensitivity. We evaluated applying less restrictive radiological criteria, when supported by specific microbiological findings, to define IPA in acute myeloid leukaemia (AML), lymphoproliferative diseases (LD) and allogeneic stem cell transplant (allo-SCT) patients. Overall, 109 consecutive episodes of proven/probable IPA in 56 AML, 31 LD and 22 allo-SCT patients diagnosed from February 2006 through to January 2011 were considered. IPA was diagnosed with EORTC-MSG criteria (control group, 76 patients) or without prespecified radiological criteria (study group, 33 patients). The latter differed from the former by the inclusion of patients with pulmonary infiltrates not fulfilling the three EORTC-MSG IPA specific findings of dense, well-circumscribed lesions with or without halo sign, air crescent sign or cavity. All the analysed clinical and mycological characteristics, 3-month response to antifungal therapy and 1- and 3-month cumulative survival were comparable in the control and study groups in AML, LD and allo-SCT patients. Seventeen of 33 (51.5%) patients of the study group fulfilled EORTC-MSG radiological criteria at subsequent imaging performed a median of 15 days (range, 6-40 days) after documentation of the pulmonary infection. Our study seems to confirm the possibility of revising the EORTC-MSG criteria by extending the radiological suspicion of IPA to less specific chest computerized tomography scan findings when supported by microbiological evidence of Aspergillus infection in high-risk haematological patients. PMID:21999366

Girmenia, C; Guerrisi, P; Frustaci, A M; Fama, A; Finolezzi, E; Perrone, S; Gentile, G; Collerone, F; Brocchieri, S; Guerrisi, V

2011-10-17

47

Haematological parameters in a neotropical fish, Corydoras paleatus (Jenyns, 1842) (Pisces, Callichthyidae), captured from pristine and polluted water  

Microsoft Academic Search

We report normal ranges of haematological indices in healthy Corydoras paleatus from an unpolluted area. Haematological parameters studied include: erythrocyte counts (Er), haematocrit (Ht), haemoglobin concentration (Hb), mean cell volume (MCV), mean cell haemoglobin (MCH) and mean cell haemoglobin concentration (MCHC). Normal red blood parameters did not change according to maturation stages, sex or seasons. Then, we compared them with

Jimena Cazenave; Daniel Alberto Wunderlin; Andrea Cecilia Hued

2005-01-01

48

Haematological and Biochemical Values or the Blood and Blood Serum of Captive Northern White Rhinoceroses (Ceratotherium simum cottoni)  

Microsoft Academic Search

Va h a I a 1., F. K d e and O. A. R Y d e r: Haematological and Biochemical Values of the Blood and Blood Serum of Captive Northern White Rhinoceroses (Ceratotherium simum cottoni). Acta vet. Brno, 63,1994: 99-102. Haematological and biochemical blood and blood serum values obtained from 7 northern white rhinoceroses (Ceratotherium simum cottoni) kept in

J. Váhala; V. Kaše; O. A. Ryder

1994-01-01

49

Catastrophic bleeds during end-of-life care in haematology: controversies from Australian research  

Microsoft Academic Search

Goals of work  This article seeks to address the lack of information in the literature, by providing preliminary findings on the experience\\u000a of managing catastrophic bleeds for haematology patients, from the perspective of health professionals involved in the care\\u000a of such patients and their families.\\u000a \\u000a \\u000a \\u000a Materials and methods  Insights from qualitative interviews with seventeen (n?=?17) haematological nurses, four (n?=?4) palliative care nurses

Pam McGrath; Michael Leahy

2009-01-01

50

Haematological and immunological responses to the tapeworm hymenolepis diminuta in man.  

PubMed

Self-infections with the tapeworm Hymenolepis diminuta were carried out to study the haematological and immunological responses in man. Infection caused pronounced eosinophilia, an increase in plasma viscosity and the production of parasite-specific IgG and IgM. Circulating IgE was not detected. There were no significant changes in erythrocyte values or serum transaminases. PMID:1162734

Turton, J A; Williamson, J R; Harris, W G

1975-06-01

51

Prevalence of rheumatic manifestations and antineutrophil cytoplasmic antibodies in haematological malignancies. A prospective study  

Microsoft Academic Search

Objective. To evaluate the prevalence of antineutrophil cytoplasmic antibodies (ANCA) and rheumatic manifestations associated with chronic haematological malignancies. Methods. Two groups of patients were prospectively studied (group I: 60 patients with myelodysplastic syndromes and group II: 140 patients with lymphoid malignancies) for clinical 'immune' manifestations and ANCA. Results. In the myelodysplastic group, six patients had ANCA-negative systemic medium- size vasculitis,

M. A. Hamidou; S. Derenne; M. A. P. Audrain; J. M. Berthelot; A. Boumalassa; J. Y. Grolleau

2000-01-01

52

Reversible phosphorylation in haematological malignancies: Potential role for protein tyrosine phosphatases in treatment?  

Microsoft Academic Search

Most aspects of leukocyte physiology are under the control of reversible tyrosine phosphorylation. It is clear that excessive phosphorylation of signal transduction elements is a pivotal element of many different pathologies including haematological malignancies and accordingly, strategies that target such phosphorylation have clinically been proven highly successful for treatment of multiple types of leukemias and lymphomas. Cellular phosphorylation status is

Roberta R. Ruela-de-Sousa; Karla C. S. Queiroz; Maikel P. Peppelenbosch; Gwenny M. Fuhler

2010-01-01

53

Effect of storage time on haematological parameters in mullet, Mugil cephalus.  

PubMed

The aim of the present study was to assess the effect of storage time at +4?°C on red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), white blood cell count (WBC), thrombocyte count (TC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in mullet (Mugil cephalus) using an automatic method. After blood collection (T0 ), all samples were analyzed using both the manual and automatic method. To test the validation of the automatic method, a paired t-test was applied, and no statistical difference was observed. The samples were successively divided into four different aliquots and stored at +4?°C to assess the haematological parameters using the automatic method. The first aliquot was refrigerated for 6 h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. One-way repeated-measures ANOVA showed a significant effect of storage time (P?haematological parameters can be assessed within 6 h from blood collection when samples are stored at +4?°C because long-term storage modifies the results of the analyses. Further studies on these parameters could be still needed in various fish species to validate an appropriate method for haematological analysis useful not only for the evaluation of the health status of animal living in captivity and in aquaculture but also to have reliability environmental haematological biomarkers. PMID:23097308

Faggio, C; Casella, S; Arfuso, F; Marafioti, S; Piccione, G; Fazio, F

2012-10-24

54

Haematological factors associated with proliferative retinopathy in homozygous sickle cell disease  

Microsoft Academic Search

Haematological indices were investigated in 261 patients with homozygous sickle cell disease, 29 of whom had developed proliferative sickle retinopathy (PSR). After allowing for age-related effects, male patients with high Hb (> 9 g\\/dl) and low HbF (< 5%) levels appeared to constitute a high risk group for PSR. This relationship was not evident in females, PSR being observed in

R. J. Hayes; P. I. Condon; G. R. Serjeant

1981-01-01

55

HAEMATOLOGY AND SERUM BIOCHEMISTRY OF CAPTIVE UNSEDATED CHITAL DEER (AXIS AXIS') IN AUSTRALIA  

Microsoft Academic Search

Haematological and serum biochemical values were measured in blood samples col- lected over a 12-mo period from 37 unsedated chital deer (Axis axis). Stags and hinds, ranging in age from birth to maturity, were sampled. Haemoglobmn, total erythrocyte and haematocrit values were low at birth and sex differences were not apparent in neonates and juveniles, but were in adults. Chital

R. S. Chapple; A. W. English; R. C. MulIey; E. E. Lepherd

1991-01-01

56

Where are we at with point-of-care testing in haematology?  

PubMed

Point-of-care testing (POCT) in haematology has continued to grow in popularity and uptake throughout the world. The increasing demand to reduce the turnaround time of test results, coupled with rapid improvements in technology, have led to the development of several devices that are designed for use in different clinical settings, with the hope of improving patient care. The most used POCT in haematology is measurement of haemoglobin concentration. Other POCT devices (used primarily in developing countries) for malaria screening and CD4+ T-lymphocytes for quantification of human-immunodeficiency-virus are becoming the cornerstone for the diagnosis and management of these disorders. New devices are also available for red cell indices, white blood cell count and platelets. In this review clinical studies that validate the use of such devices will be discussed, as well as the advantages and disadvantages of POCT in haematology. A disadvantage of POCT is a lack of training, poor standardization in obtaining blood samples and insufficient internal/external quality assessment. As there is every reason to expect that POCT use will increase in all pathology disciplines, including haematology, it is imperative that systems are put in place to oversee these issues. PMID:22765160

Briggs, Carol; Kimber, Simon; Green, Laura

2012-07-05

57

Studies on an ascitic syndrome in young broilers 1. haematology and pathology  

Microsoft Academic Search

In the present investigation, the haematology and pathology of young broiler chicks with an ascitic syndrome were examined.Gross changes included cardiomegaly, ascites, congested lungs, shrunken livers, enlarged kidneys, dark breast muscle and congested intestines. Many blood parameters were significantly raised including the packed cell volume (PCV), haemoglobin (Hb), red (Rbc) and white blood cell (Wbc) counts. The heterophils and monocytes

M. H. Maxwell; G. W. Robertson; S. Spence

1986-01-01

58

Seasonal variation in haematological parameters in male and female Tinca tinca  

Microsoft Academic Search

This study was designed to investigate any seasonal (spring, summer, autumn and winter) changes in haematological parameters in the blood of Tinca tinca measuring the number of red blood cells (RBC), haematocrit, white blood cells, and total plasma proteins.The results show significant changes in RBC and haematocrit in males comparing spring and summer with autumn and winter, whereas in females

María Eugenia Collazos; Eduardo Ortega; Carmen Barriga; Ana Beatriz Rodrěguez

1998-01-01

59

Toxic effects of some plants in the genus Euphorbia on haematological and biochemical parameters of rats  

Microsoft Academic Search

ADEDAPO, A. A., M. O. ABATAN, O. O. OLORUNSOGO: Toxic effects of some plants in the genus Euphorbia on haematological and biochemical parameters of rats. Vet. arhiv 74, 53-62, 2004. ABSTRACT The toxic effects of 5 suspected poisonous plants of the genus Euphorbia (Euphorbia balsamifera Aiton, E. heterophylla L., E. hirta L., E. hyssopifolia L., and E. lateriflora Schum and

Adeolu A. Adedapo; Matthew O. Abatan; Olufunso O. Olorunsogo

2004-01-01

60

Effects of oral iron supplementation on some haematological parameters and iron biochemistry in neonatal dairy calves  

Microsoft Academic Search

Since the iron requirements of ruminants are not well established and most recommendations are estimates, this study was performed to determine whether 150mg\\/day iron supplementation in neonatal dairy calves (heifer replacement system) fed roughage and concentrates could promote a healthy haematology and iron profile. A total of 40 calves was used in the present study. The animals were divided into

M. Mohri; F. Sarrafzadeh; H. A. Seifi; N. Farzaneh

2004-01-01

61

Changes in haematological parameters and iron metabolism associated with a 1600 kilometre ultramarathon  

Microsoft Academic Search

OBJECTIVE: To investigate haematological variations and iron related changes in the serum of participants in a 1600 kilometre ultramarathon run. PARTICIPANTS: Seven male and two female participants in a 1600 km foot race. METHODS: Blood samples were obtained from the participants before, after four and 11 days of running, and at the end of the event. Samples were analysed by

K. E. Fallon; G. Sivyer; K. Sivyer; A. Dare

1999-01-01

62

Effect of acute exercise on some haematological parameters and neutrophil functions in active and inactive subjects  

Microsoft Academic Search

In this work we studied the possible effects of acute exercise on some haematological parameters and on some functions of neutrophils in seven active and six inactive subjects. Physical exercise (10 min on a cycle ergometer at a heart rate of 150 beats · min–1) induced a significant increase in total leucocyte, lymphocyte and neutrophil concentrations in active subjects; serum

G. Benoni; P. Bellavite; A. Adami; S. Chirumbolo; G. Lippi; G. Brocco; L. Cuzzolin

1995-01-01

63

Haematological changes in two thoroughbred horses in training with confirmed equine herpesvirus 1 infections  

Microsoft Academic Search

An outbreak of respiratory disease among thoroughbred horses in training in Hong Kong was caused by equine herpesvirus 1 (EHV-1) subtype 1 (abortion strain). Two of the horses affected by EHV-1 were serially blood sampled over a period of several weeks and their haematological values measured. There was an increase in monocyte count in the first few days which steadily

DK Mason; KL Watkins; CM Luk

1989-01-01

64

Comparative haematological and plasma chemistry values in purpose-bred squirrel, cynomolgus and rhesus monkeys  

Microsoft Academic Search

Haematological and blood chemistry data have been compared for three species of purpose-bred primates, Macaca mulatta (rhesus monkey), Macaca fascicularis (cynomolgus monkey), Saimiri sciureus (squirrel monkey). These species were housed over a 9-month period in identical conditions (diet, tap water, room temperature and relative humidity, and lighting regime) in our primate facility. Blood and urine assays were conducted using the

T. Matsuzawa; Y. Nagai

1994-01-01

65

Oral health and quality of life among patients with head and neck cancer or haematological malignancies  

Microsoft Academic Search

The aim of the present study was to monitor the health-related quality of life (HRQOL) of patients with head and neck cancer or haematological malignancies during a period from before to after medical treatment, and to investigate the relationships between patient experiences of oral symptoms and HRQOL. The sample consisted of 41 consecutive patients. At the start, during and end

Kerstin E. O. Öhrn; Per-Olow Sjödén; Ylva-Britt Wahlin; Marie Elf

2001-01-01

66

Complementary and alternative medicine use in patients with haematological malignancies in Europe.  

PubMed

This study reports upon a descriptive cross-sectional survey assessing the use of complementary and alternative medicine (CAM) in patients with haematological cancers. Twelve European countries contributed data from patients with haematological cancers, as part of a larger study. Sixty-eight patients with haematological cancer participated. Among the participants, 26.5% used some form of CAM after the cancer diagnosis. The most common therapies used were homeopathy (38.9%), herbal medicine (22.2%) various psychic therapies, such as use of mediums, healers, rebirthing or past life regression therapy (22.2%). A particular profile of a CAM user was not evident in the sample. Moderate levels of satisfaction with CAM were reported. Patients commonly used CAM to increase the ability of their body to fight cancer and to improve physical and emotional well-being. Information about CAM was received mainly from friends or family. As CAM use in patients with haematological malignancies is common, clinicians should assist patients who want to use CAM to make an appropriate decision, and improve communication with them about CAM use in an open and non-judgemental dialogue. PMID:15955292

Molassiotis, Alexander; Margulies, Anita; Fernandez-Ortega, Paz; Pud, Dorit; Panteli, Vassiliki; Bruyns, Ingrid; Scott, Julia A; Gudmundsdottir, Gudbjorg; Browall, Maria; Madsen, Elin; Ozden, Gulten; Magri, Miriam; Selvekerova, Sarka; Platin, Nurgun; Kearney, Nora; Patiraki, Elisabeth

2005-05-01

67

Clinical, ultrasonography and haematology of aglepristone-induced mid-gestation pregnancy terminations in rabbits.  

PubMed

Aglepristone is a safe abortifacient in cats, dogs and rabbits. Although no serious side effects have been reported, there is no information available about the effects of the medicine on haematological parameters. For the first time clinical and ultrasonographic features and haematological profiles were evaluated in rabbits treated with aglepristone 15 and 16 days after mating. Ten healthy 10-14 month-old New Zealand White female rabbits were mated with fertile bucks and pregnancies were con?rmed by ultrasound 15 days later. Of these, 5 does were treated with aglepristone (test group, n = 5) whilst the remaining five (control group, n = 5) were treated with a saline solution (0.9% NaCl). The treatment dose was 10 mg/kg body weight, administered subcutaneously once daily on two consecutive days (day 15 and 16 post mating). Ultrasonographic, clinical and haematological assessments were performed daily. Aglepristone treatment induced embryonic fluid resorptions without foetal death in mid-gestation terminations. Following ultrasonographic and haematological examinations, it was established that aglepristone is a safe abortifacient in rabbits. PMID:23718772

Özalp, Gözde R; Temizel, Ethem M; Özocak-Batmaz, Elçin

2013-05-06

68

Dielectric, haematological and biochemical studies of detergent toxicity in fish blood  

Microsoft Academic Search

Blood characteristics in dielectric, haematological and biochemical terms of the fish Cyprinus carpio exposed to a sublethal concentration of sodium alkyl benzene sulphonate were compared with those from untreated control fish; recovery from the test solution was also checked. Trends of change in the majority of chosen parameters of blood with time of fish exposure to anionic detergent were significantly

I. Bielinska

1987-01-01

69

Arbitrary primed PCR rules out Clostridium difficile cross-infection among patients in a haematology unit  

Microsoft Academic Search

Eight out of 20 (40%) patients with haematological malignancies hospitalized in the same unit of our hospital from 24 January to 24 April 1995, suffered from diarrhoea due to Clostridium difficile. The C. difficile isolates were characterized by serotyping and by arbitrary primed polymerase chain reaction (AP-PCR) using three different 10-mer oligonucleotides. It was found by serotyping that five patients

F. Lemann; C. Chambon; F. Barbut; C. Gardin; J. Bričre; N. Lambert-Zechovsky; C. Branger

1997-01-01

70

Haematological findings in budgerigars with megabacterium and Trichomonas infections associated with 'going light'  

Microsoft Academic Search

Eighteen budgerigars with clinical signs of 'going light' were euthanased and examined post mortem; ingluvitis caused by Trichomonas gallinae infection was present in seven birds, proventriculitis associated with the presence of megabacteria in eight birds and in three birds both conditions were present. Haematological examinations of blood taken from the living birds showed that those with T gallinae infection had

GM Henderson; FM Gulland; CM Hawkey

1988-01-01

71

Herpes simplex virus and intraoral ulcers in immunocompromised patients with haematologic malignancies  

Microsoft Academic Search

Possible factors predisposing to the development of intraoral ulcers in immunocompromised patients with haematologic malignancies were investigated. Among 46 patients undergoing antineoplastic treatment, 18 developed an intraoral ulcer during the prospective study. Patients with or without ulcers were comparable with respect to underlying disease, presence of teeth, the qualitative composition of the aerobic and facultatively anaerobic oral microflora, herpes simplex

O. J. Bergmann; S. C. Mogensen; J. Ellegaard

1990-01-01

72

The haematological, biochemical and immunological profile of athletes suffering from the overtraining syndrome  

Microsoft Academic Search

To help clarify the overtraining syndrome (OTS), a combination of parameters were measured in ten athletes who were suffering from OTS. Blood samples were obtained at rest and a range of haematological, biochemical and immunological tests were carried out on the samples. For each parameter, the mean value for the group was compared to an established normal range amongst age-matched

David G. Rowbottom; David Keast; Carmel Goodman; Alan R. Morton

1995-01-01

73

Paul Ehrlich (1854-1915): founder of chemotherapy and pioneer of haematology, immunology and oncology.  

PubMed

The Jewish-German histologist, microbiologist, bio- chemist, immunologist and pharmacologist Paul Ehrlich, by his research founded chemotherapy and pioneered im- munology, haematology and oncology. For his contribu- tion in immunology he was awarded the Nobel Prize of Medicine and Physiology in 1908. PMID:17415859

Androutsos, G

74

Effects of 2Phenoxyethanol Anaesthesia on Haematological Profi le on Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss)  

Microsoft Academic Search

Velíšek J., Z. Svobodová, V. Pia?ková: Effects of 2-Phenoxyethanol Anaesthesia on Haematological Profi le on Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss). Acta Vet. Brno 2007, 76: 487-492. The aim of this study was to assess changes in haematological profi le of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) after the exposure to anaesthetic 2-phenoxyethanol. The

Z. SVOBODOVÁ

2007-01-01

75

Factors influencing haematological recovery after allogeneic bone marrow transplantation in leukaemia patients treated with methotrexate-containing GVHD prophylaxis  

Microsoft Academic Search

In the present single institution study of 66 leukaemia patients (28 AML, 23 ALL, 15 CML), the factors influencing haematological\\u000a recovery after allogeneic bone marrow transplantation (alloBMT) were analysed retrospectively to identify the optimal conditions\\u000a required for rapid haematological recovery after alloBMT. All patients received GVHD prophylaxis with cyclosporine A plus\\u000a methotrexate. The mean number of days required to achieve

H. T. Hassan; C. Krog; M. Stockschläder; W. Zeller; W. Krüger; R. Erttmann; A. R. Zander

1997-01-01

76

Haematological and blood biochemical characteristics of Glyptosternum maculatum (Siluriformes: Sisoridae) in Xizang (Tibet).  

PubMed

This study investigated the haematological and blood biochemical characteristics of Glyptosternum maculatum. The haematological and biochemical parameters were measured in 30 adult fish collected from Nyingchi Reach of Yarlung Zangbo River in Tibet. The red blood cell count (RBC), haemoglobin concentration (Hb), haematocrit (Hct), erythrocyte osmotic fragility (maxEof and minEof), the erythrocyte sedimentation rate, mean cell volume (MCV), mean cellular haemoglobin content (MCH), and mean cell haemoglobin concentration (MCHC) were determined. Compared with other Siluriformes fishes, G. maculatum showed similar mean values for Hct, Hb, MCH, and MCHC and had slightly lower RBC and higher MCV. The biochemical parameters were assayed including alanine aminotransferase, aspartate aminotransferase (AST), alkaline phosphatase, total protein, albumin, globulin, albumin/globulin ratio, total bilirubin, direct bilirubin, urea, creatinine, glucose, total cholesterol, and triglyceride. The result showed that the value of AST in G. maculatum was obviously higher than that in Rhamdia quelen as well as in Silurus merdionalis. PMID:19757131

Zhang, Huijuan; Xie, Congxin; Li, Dapeng; Xiong, Dongmei; Liu, Haiping; Suolang, Sizhu; Shang, Peng

2009-09-10

77

Leukaemic transformation in patients with haematological disease receiving tumour necrosis factor inhibitors.  

PubMed

In recent years, there has been an increase in the use of tumour necrosis factor (TNF) inhibitors as treatment for several inflammatory conditions. However, the question of whether TNF inhibitors increase the risk of malignancies (including lymphoma and leukaemia) in these diseases remains controversial. Despite this concern, anti-TNF therapy is being used experimentally in the management of haematological patients with risk of leukaemic transformation such as myeloproliferative neoplasms. We report here the first ever reported case of blastic transformation in a patient with myelofibrosis under etanercept treatment for a severe hidradenitis suppurativa. Although etanercept provided a sustained partial response of the skin disease, the patient developed an acute myeloid leukaemia after 27 months on exclusively etanercept therapy. According to the Dynamic International Prognostic Scoring System-plus score, the patient had a low risk for leukaemic transformation. We discuss here the potential of TNF inhibitors to increase the already elevated risk of leukaemic transformation of these haematological diseases. PMID:22550963

Ferrer-Marín, Francisca; Amigo, Maria Luz; Vicente, Vicente

2012-06-01

78

Haemoglobin Lepore Boston-Washington in Sicily: clinical, haematological, and biosynthetic studies.  

PubMed Central

In the south-east of Sicily 23 children from 14 unrelated families have been diagnosed as suffering from haemoglobin Lepore. Such a high incidence shows that Sicily is an important focus of haemoglobin Lepore. The results of haematological and biosynthetic studies in 18 carriers of Hb Lepore and in five double heterozygotes for Hb Lepore and beta-thalassaemia are presented. In the carriers the haematological and biosynthetic data are compared with carriers of beta-thalassaemia, while the five double heterozygotes are compared with beta o- and beta +-thalassaemia major subjects. In the carriers of Hb Lepore no synthesis of delta beta-chains was observed in peripheral blood cells; in fact we found a peak in the bone marrow. Double heterozygotes with circulating nucleated red cells showed delta beta-chain synthesis in peripheral blood.

Schiliro, G; Musumeci, S; Pizzarelli, G; Fischer, A; Romero, M A; Russo, G

1980-01-01

79

Increased bone marrow microvascular density in haematological malignancies is associated with differential regulation of angiogenic factors  

Microsoft Academic Search

Antiangiogenic drugs are currently tested in haematological malignancies. As these drugs target different angiogenic regulators, and as cancers are inherently heterogeneous, a detailed characterization of angiogenesis in individual cancers is needed. Hence, we measured bone marrow microvessel density (MVD), plasma concentrations of eight angiogenesis-related parameters and the expression in blood mononuclear cells of 40 angiogenesis-related mRNAs in 93 patients with

H F S Negaard; N Iversen; I M Bowitz-Lothe; P M Sandset; B Steinsvik; B Řstenstad; P O Iversen; HFS Negaard

2009-01-01

80

Haematological and spermatotoxic effects of ethylene glycol monomethyl ether in copper clad laminate factories  

PubMed Central

OBJECTIVES—To investigate the effects of ethylene glycol monomethyl ether (EGME) on haematology and reproduction in exposed workers.?METHODS—53 Impregnation workers from two factories that make copper clad laminate with EGME as a solvent were recruited as the exposed group. Another group of 121 lamination workers with indirect exposure to EGME was recruited as the control group. Environmental monitoring of concentrations of EGME in air and biological monitoring of urinary methoxyacetic acid (MAA) concentrations were performed. Venous blood was collected for routine and biochemical analyses. Semen was collected from 14 workers exposed to EGME for sperm analysis and was compared with 13 control workers.?RESULTS—Results of haematological examination showed that the haemoglobin, packed cell volume, and red blood cell count in the male workers exposed to EGME were significantly lower than in the controls. The frequency of anaemia in the exposed group (26.1%) was significantly higher than in the control group (3.2%). However, no differences were found between the female workers exposed and not exposed to EGME. After adjustment for sex, body mass index, and duration of employment, red blood cell count was significantly negatively associated with air concentrations of EGME, and haemoglobin, packed cell volume, and red blood cell count were significantly negatively associated with urinary concentrations of MAA. The pH of semen in the exposed workers was significantly lower than in the control workers, but there were no significant differences in the sperm count or sperm morphology between the exposed and control groups.?CONCLUSION—It can be concluded that EGME is a haematological toxin, which leads to anaemia in the exposed workers. However, the data from this study did not support the theory of a spermatotoxic effect of EGME.???Keywords: ethylene glycol monomethyl ether; haematological disorders; spermatotoxicity

Shih, T.; Hsieh, A.; Liao, G.; Chen, Y.; Liou, S.

2000-01-01

81

WELFARE AND HAEMATOLOGICAL INDICES OF WEANER RABBITS AS AFFECTED BY STOCKING DENSITY  

Microsoft Academic Search

Forty two weaner rabbits of mixed breeds and sexes with an average initial weight of 956.7±45.6 g were used to evaluate the effect of stocking densit y on welfare and haematological characteristics. Th e rabbits were allotted to four stocking densities of 10, 14.3, 20 and 25 rabbits\\/m 2. This corresponded to 2, 3, 4 and 5 rabbits per cage

82

Haematological and immunological response of calves to infection with Taenia saginata  

Microsoft Academic Search

Calves were infected with eggs ofTaenia saginata and the haematologic response and the antigen-induced responsiveness of their peripheral blood lymphocytes was followed. No changes were observed in the erythrocyte levels, but a leukocytosis developed. This was contributed to primarily by a lymphocytosis, but also by an eosinophilia. Antigen-induced lymphocyte blastogenesis measured the antigen-sensitive cell population in peripheral blood and this

S. Lloyd

1980-01-01

83

A data management software for the Sysmex NE 8000 haematology analyser  

Microsoft Academic Search

The Sysmex NE 8000 (TOA-Japan) is a haematology analyser that performs blood cells count and leukocyte differential count. For facilitating the work of technical validation, we developed a software adapted to any IBM® or compatible PC running under MS-DOS®, to manage the analyser. Data are automatically collected via the RS-232 interface from the analyser or keyed in for the other

J. P. Cambus; F. Nguyen; F. Cambus

1996-01-01

84

Effects of some plants of the spurge family on haematological and biochemical parameters in rats  

Microsoft Academic Search

AdEdApO, A. A., M. O. AbA t An, O. O. OlOrunsOgO: Effects of some plants of the spurge family on haematological and biochemical parameters in rats. Vet. arhiv 77, 29-38, 2007. AbstrAct The effects of five suspected poisonous plants of the spurge family ( euphorbiaceae ) i.e. alchornea cordifolia Schum and Thorn, Cnidoscolus acontifolius Mill, Phyllanthus amarus Schum and Thorn,

Adeolu Alex Adedapo; Matthew Oluwole Abatan; Olufunso Olabode Olorunsogo

85

Alterations in Coagulation Profiles and Biochemical and Haematological Parameters in Cattle with Traumatic Reticuloperitonitis  

Microsoft Academic Search

This study was designed to investigate alterations in coagulation, and in biochemical and haematological parameters in cattle\\u000a with traumatic reticuloperitonitis (TRP). In the study, 28 dairy cattle with TRP and 10 clinically healthy cattle (control)\\u000a of different ages and breeds were used. Cattle with TRP had prolonged prothrombin time (PT), thrombin time (TT) and activated\\u000a partial thromboplastin time (APTT). Erythrocytopenia,

H. I. Gokce; G. Gokce; M. Cihan

2007-01-01

86

Haematological Alterations Observed in Equine Routine Complete Blood Counts. A Retrospective Investigation  

Microsoft Academic Search

:   Results on more than 1000 complete blood counts (CBC) performed on hot-blooded horses, including Thoroughbred, Standardbred,\\u000a and Arabian, have been reviewed. All the data were processed and stored at the Laboratory of Haematology, Department Veterinary\\u000a Clinic, University of Pisa, over the last 2 years, and involved samples withdrawn from both inpatients and outpatients of\\u000a the clinic.\\u000a \\u000a ?The CBC is

A. GAVAZZA; A. J. DELGADILLO; B. GUGLIUCCI; A. PASQUINI; G. LUBAS

2002-01-01

87

Haematological and haematochemical responses to training and competition in standardbred horses  

Microsoft Academic Search

The aim of this study was to evaluate haematological and haematochemical responses to training and competition in standardbred\\u000a horses. We investigated the modifications that occur in athletic horses during different workloads of training and after official\\u000a competitions of 1,600 and 2,000 m. The study was carried out on 15 gelding clinically healthy horses. The animals were divided\\u000a into three groups: group

Giuseppe Piccione; Stefania Casella; Claudia Giannetto; Vanessa Messina; Vincenzo Monteverde; Giovanni Caola; Sergio Guttadauro

2010-01-01

88

Allogeneic transplant outcomes are not affected by body mass index (BMI) in patients with haematological malignancies  

Microsoft Academic Search

Bone marrow transplantation is frequently used as a consolidation therapy in patients with haematological malignancies to\\u000a improve the outcome of these patients. Obese individuals have larger absolute lean body and fat masses than non-obese individuals\\u000a of the same age, gender and height, which might lead to altered pharmacokinetics of chemotherapeutic agents. Data on the impact\\u000a of body mass on transplant

Emmanouil Nikolousis; Sandeep Nagra; Shankara Paneesha; Julio Delgado; Kathy Holder; Lynn Bratby; Sridhar Chaganti; Richard Lovell; Donald Milligan

2010-01-01

89

Erythropoietin acute reaction and haematological adaptations to short, intermittent hypobaric hypoxia  

Microsoft Academic Search

This study aimed to determine whether brief hypoxic stimuli in a hypobaric chamber are able to elicit erythropoietin (EPO)\\u000a secretion, and to effectively stimulate erythropoiesis in the short term. In two different experiments, a set of haematological,\\u000a biochemical, haemorheological, aerobic performance, and medical tests were performed in two groups of healthy subjects. In\\u000a the first experiment, the mean plasma concentration

Ferran A. Rodríguez; Josep L. Ventura; Mireia Casas; Héctor Casas; Teresa Pagés; Ramón Rama; Antoni Ricart; Luis Palacios; Ginés Viscor

2000-01-01

90

Haematological analysis conducted at the departure of the Tour de France 2001.  

PubMed

Since the introduction of blood analysis performed before major cycling events in 1997, there have been discussions concerning the quality of the results. The aim of our study therefore was to measure blood samples and compare the results obtained on the field with those obtained in the laboratory. For this it was necessary to have blood samples analysed with different instruments to determine the exactness of the results and evaluate the performances of these instruments in order to validate the haematological testing used to reveal athletes abusing recombinant erythropoietin. We report on the haematological analysis of 177 professional cyclists who took part in the Tour de France 2001. All the blood samples were withdrawn in the morning between 7 and 9 am in Dunkerque (France) and were analysed immediately with a transportable analyser. Then the samples were flown to Lausanne (Switzerland) and were reanalysed in two independent ISO 17 025 accredited laboratories with three different analysers. The results confirmed that the most effective haematological follow-up should be performed under standardized pre-analytical conditions and with identical analysers of the same manufacturer to avoid too many variations notably on the haematocrit level and the reticulocyte count. Furthermore, this study suggests that analyses performed on the site are good and could enable the federations to perform a urinary test to detect rhEPO abuse right after the blood analysis. This time saving is essential to fight efficiently recombinant erythropoietin doping, because the half life of the hormone is very short. PMID:15776340

Robinson, N; Schattenberg, L; Zorzoli, M; Mangin, P; Saugy, M

2005-04-01

91

The efficacy of nardostachys jatamansi against the radiation induced haematological damage in rats.  

PubMed

Introduction: Radiation is increasingly being used for medical purposes and it is an established weapon in the diagnosis and the therapy of cancer. An exposure to 1-2 Gys causes the NVD (Nausea, vomiting, diarrhoea) syndrome, whereas an exposure to 2-6 Gys causes the haematopoietic syndrome. The aim of the present study was to investigate the protective effect of the Nardostachys jatamansi root extract (NJE) on the radiation induced haematological damage in rats. Materials and Methods: EBR was performed at the Microtron Centre, Mangalore University, India. Rats were treated with NJE once daily for 15 days before and after the irradiation. After the irradiation, blood was collected for determining the peripheral blood counts (RBC and WBC), haemoglobin, the platelet count and the packed cell volume (PCV) at 6 hours, 12 hours, 24 hours, 48 hours and 5, 10 and 15 days post irradiation. The data was analyzed by one way ANOVA, followed by the Tukey's test for multiple comparisons. Result: NJE provided protection against the radiation induced haematological disorders. The rats treated with NJE exhibited a time dependent significant elevation in all the haematological parameters which were studied and its modulation upto the near normal level was recorded. Conclusion: From this study, we concluded that, NJE provides protection by modulating the radiation induced damage on the haematopoietic system. PMID:23905085

Gowda, Damodara K M; Shetty, Lathika; A P, Krishna; Kumari, Suchetha N; Sanjeev, Ganesh; P, Naveen

2013-04-26

92

Immune reconstitution in children following chemotherapy for haematological malignancies: a long-term follow-up.  

PubMed

Modern intensive chemotherapy for childhood haematological malignancies has led to high cure rates, but has detrimental effects on the immune system. There is little knowledge concerning long-term recovery of the adaptive immune system. Here we studied the long-term reconstitution of the adaptive immune system in 31 children treated for haematological malignancies between July 2000 and October 2006. We performed detailed phenotypical and functional analyses of the various B and T cell subpopulations until 5 years after chemotherapy. We show that recovery of newly-developed transitional B cells and naive B and T cells occurred rapidly, within months, whereas recovery of the different memory B and T cell subpopulations was slower and incomplete, even after 5 years post-chemotherapy. The speed of B and T cell recovery was age-independent, despite a significant contribution of the thymus to T cell recovery. Plasmablast B cell levels remained above normal and immunoglobulin levels normalised within 1 week. Functional T cell responses were normal, even within the first year post-chemotherapy. This study shows that after intensive chemotherapy for haematological malignancies in children, numbers of several memory B and T cell subpopulations were decreased on the long term, while functional T cell responses were not compromised. PMID:21114483

van Tilburg, Cornelis M; van Gent, Rogier; Bierings, Marc B; Otto, Sigrid A; Sanders, Elisabeth A M; Nibbelke, Elisabeth E; Gaiser, Jacobus F; Janssens-Korpela, Pirkko L; Wolfs, Tom F W; Bloem, Andries C; Borghans, José A M; Tesselaar, Kiki

2010-11-28

93

Clinical, haematological, biochemical and economic impacts of Trichinella spiralis infection in pigs.  

PubMed

The purpose of this work was to assess the clinical, haematological and biochemical responses of pigs experimentally inoculated with Trichinella spiralis. Groups of three pigs were inoculated per os with 100, 500 and 5000 T. spiralis muscle larvae, two pigs were used as control. Clinical evaluation of disease in pigs included daily examination, rectal temperature measurements and cardiac and respiration rates. Haematological studies included: hematocrit (%), hemoglobin (g/dl), and white cell, neutrophil, lymphocyte and eosinophil counts. Blood biochemistry included: bun (mg/dl), creatinine (mg/dl), AST (UI/l), ALT (UI/l), CPK (UI/l) and ALP (UI/l). No significant differences were observed in rectal temperature and in cardiac and respiration rates between inoculated animals and the control group (p> or =0.05). Significant differences were detected (p< or =0.05) in the values of % hemoglobin, and eosinophils, as well as in the values of CK, ALP, AST and ALT. The variations observed in some cases were related to the number of T. spiralis larvae inoculated and varied with the number of days post-infection. Inoculated pigs showed significant differences (p< or =0.05) in weight gain when compared with uninoculated controls. This study has clinical, haematological, and enzyme alterations in Trichinella infected pigs provides a better understanding of acute and chronic trichinellosis in pigs. PMID:17543458

Ribicich, M; Gamble, H R; Rosa, A; Sommerfelt, I; Marquez, A; Mira, G; Cardillo, N; Cattaneo, M L; Falzoni, E; Franco, A

2007-05-31

94

Alcohol drinking, tobacco smoking and subtypes of haematological malignancy in the UK Million Women Study  

PubMed Central

Background: Previous research suggests associations of lower alcohol intake and higher tobacco consumption with increased risks of haematological malignancy. The prospective Million Women Study provides sufficient power for reliable estimates of subtype-specific associations in women. Methods: Approximately 1.3 million middle-aged women were recruited in the United Kingdom during 1996–2001 and followed for death, emigration and cancer registration until 2009 (mean 10.3 years per woman); potential risk factors were assessed by questionnaire. Adjusted relative risks were estimated by Cox regression. Results: During follow-up, 9162 incident cases of haematological malignancy were recorded, including 7047 lymphoid and 2072 myeloid cancers. Among predominantly moderate alcohol drinkers, higher intake was associated with lower risk of lymphoid malignancies, in particular diffuse large B-cell lymphoma (relative risk 0.85 per 10?g alcohol per day (95% confidence interval 0.75–0.96)), follicular lymphoma (0.86 (0.76–0.98)) and plasma cell neoplasms (0.86 (0.77–0.96)). Among never- and current smokers, higher cigarette consumption was associated with increased risk of Hodgkin lymphoma (1.45 per 10 cigarettes per day (1.22–1.72)), mature T-cell malignancies (1.38 (1.10–1.73)) and myeloproliferative/myelodysplastic disease (1.42 (1.31–1.55)). Conclusion: These findings confirm and extend existing evidence for associations of subtypes of haematological malignancy with two common exposures in women.

Kroll, M E; Murphy, F; Pirie, K; Reeves, G K; Green, J; Beral, V

2012-01-01

95

Haematological and biochemical toxicity induced by methanol in rats: ameliorative effects of Opuntia vulgaris fruit extract.  

PubMed

The ameliorative effects of Opuntia vulgaris fruit extract (OE) were evaluated against methanol-induced haematological and biochemical toxicity in rats. The methanol-induced haematological and biochemical perturbation significantly decreased the levels of red blood cell (RBC), haemoglobin (Hb), haematocrit (Ht), serum total protein and increased glucose, cholesterol, and triglyceride levels in serum. Treatment of rats with methanol significantly increased lipid peroxidation (LPO) level and decreased the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in erythrocytes. OE treatment could increase significantly the levels of RBC, Hb, Ht and total protein, and decrease glucose, cholesterol and triglyceride levels in serum, and increase the activities of SOD, CAT and GPx in erythrocytes, when compared with methanol-treated group. Spleen histopathology showed that OE could significantly reduce the incidence of spleen lesion induced by methanol. These results suggested that OE could exhibit a potential source of natural antioxidants against methanol-induced haematological and biochemical disruption in rats. The protective effects of OE may be due to the modulation of antioxidant enzymes activities and inhibition of LPO. PMID:21422078

Saoudi, Mongi; Jebahi, Samira; Jamoussi, Kamel; Ben Salah, Ghada; Kallel, Choumous; El Feki, Abdelfattah

2011-03-21

96

The influence of administering "effective microorganisms" to pullets on chosen haematological and biochemical blood indexes.  

PubMed

"Effective Microorganisms" (EM)--a mixture of lactic acid bacteria, photosynthetic bacteria, yeasts and fungi are used mainly in agriculture and organic waste treatment. Recently, they have also been added to water and feed for animals, as well as to processing their excrements into compost and to eliminate the stench. The objective of the present study was to assess the influence of a 14-day administration of an EM solution in drinking water to layer hens on chosen haematological and biochemical indexes. The research was carried out on 120 hens divided into two equal groups. The birds in the experimental group were given drinking water with dissolved EM (5% solution), and those in the control group--water without the preparation. On the 64th day of the aviculture, the hens were weighted and their blood was taken from the wing vein for haematological and biochemical examinations. Administering EM with water to hens did not influence significantly their body weight nor chosen haematological and biochemical indexes. A significant increase was found only in the number of platelets, the level of albumins, the content of total cholesterol and the LDH activity, however, a decrease in the ALT activity was observed. PMID:20169927

Sokó?, R; Michalczyk, M; Spodniewska, A; Barski, D

2009-01-01

97

Incidence of haematological malignancies by ethnic group in England, 2001-7.  

PubMed

The aetiology of most haematological malignancies is largely unknown. Studies of migrant populations can provide insights into the relative importance of genetic and environmental risk factors for these diseases. This study compares incidence rates in British Indians, Pakistanis, Bangladeshis, Black Africans, Black Caribbeans, Chinese and Whites in England from 2001 to 2007. We analysed 134 302 haematological cancer registrations with ethnicity obtained by linkage to the Hospital Episodes Statistics database. Mid-year population estimates from 2001 to 2007 were used. Incidence rate ratios adjusted for age, sex and income were calculated, comparing the six ethnic groups to Whites and to each other. Whites had the highest rates for most subtypes. However, Blacks experienced more than double the incidence of plasma cell and mature T-cell neoplasms compared to other ethnic groups. There were also significant differences in incidence between Indians, Pakistanis and Bangladeshis for Hodgkin lymphoma and mature B-cell neoplasms and between Black African and Black Caribbeans for mature B-cell and other lymphoid neoplasms (all P < 0·001). Our results show that the risk of haematological cancers varies greatly by ethnic group, including within those groups that have traditionally been grouped together (South Asians and Blacks) with many of these differences not explicable by known risk factors. PMID:24033296

Shirley, Megan H; Sayeed, Shameq; Barnes, Isobel; Finlayson, Alexander; Ali, Raghib

2013-09-14

98

Stability of haematological parameters and its relevance on the athlete's biological passport model.  

PubMed

The stability of haematological parameters is crucial to guarantee accurate and reliable data for implementing and interpreting the athlete's biological passport (ABP). In this model, the values of haemoglobin, reticulocytes and out-of-doping period (OFF)-score (Hb-60?Ret) are used to monitor the possible variations of those parameters, and also to compare the thresholds developed by the statistical model for the single athlete on the basis of its personal values and the variance of parameters in the modal group. Nevertheless, a critical review of the current scientific literature dealing with the stability of the haematological parameters included in the ABP programme, and which are used for evaluating the probability of anomalies in the athlete's profile, is currently lacking. In addition, we collected information from published studies, in order to supply a useful, practical and updated review to sports physicians and haematologists. There are some parameters that are highly stable, such as haemoglobin and erythrocytes (red blood cells [RBCs]), whereas others, (e.g. reticulocytes, mean RBC volume and haematocrit) appear less stable. Regardless of the methodology, the stability of haematological parameters is improved by sample refrigeration. The stability of all parameters is highly affected from high storage temperatures, whereas the stability of RBCs and haematocrit is affected by initial freezing followed by refrigeration. Transport and rotation of tubes do not substantially influence any haematological parameter except for reticulocytes. In all the studies we reviewed that used Sysmex instrumentation, which is recommended for ABP measurements, stability was shown for 72 hours at 4 ° C for haemoglobin, RBCs and mean curpuscular haemoglobin concentration (MCHC); up to 48 hours for reticulocytes; and up to 24 hours for haematocrit. In one study, Sysmex instrumentation shows stability extended up to 72 hours at 4 ° C for all the parameters. There are significant differences among methods and instruments: Siemens Advia shows lower stability than Sysmex as regards to reticulocytes. However, the limit of 36 hours from blood collection to analysis as recommended by ABP scientists is reasonable to guarantee analytical quality, when samples are transported at 4 ° C and are accompanied by a certified steadiness of this temperature. There are some parameters that are highly stable, such as haemoglobin and RBCs; whereas others, such as reticulocytes, mean cell volume and haematocrit are more unstable. The stability of haematological parameters might be improved independently from the analytical methodology, by refrigeration of the specimens. PMID:22060177

Lombardi, Giovanni; Lanteri, Patrizia; Colombini, Alessandra; Lippi, Giuseppe; Banfi, Giuseppe

2011-12-01

99

High-dose carboplatin, etoposide and melphalan (CEM) with peripheral blood progenitor cell support as late intensification for high-risk cancer: non-haematological, haematological toxicities and role of growth factor administration.  

PubMed Central

The present report describes the non-haematological toxicity and the influence of growth factor administration on haematological toxicity and haematopoietic recovery observed after high-dose carboplatin (1200 mg m(-2)), etoposide (900 mg m(-2)) and melphalan (100 mg m(-2)) (CEM) followed by peripheral blood progenitor cell transplantation (PBPCT) in 40 patients with high-risk cancer during their first-line treatment. PBPCs were collected during the previous outpatient induction chemotherapy programme by leukaphereses. CEM administration with PBPCT was associated with low non-haematological toxicity and the only significant toxicity consisted of a reversible grade III/IV increase in liver enzymes in 32% of the patients. Haematopoietic recovery was very fast in all patients and the administration of granulocyte colony-stimulating factor (G-CSF) plus erythropoietin (EPO) or granulocyte-macrophage colony-stimulating factor (GM-CSF) plus EPO after PBPCT significantly reduced haematological toxicity, abrogated antibiotic administration during neutropenia and significantly reduced hospital stay and patient's hospital charge compared with patients treated with PBPCT only. None of the patients died early of CEM plus PBPCT-related complications. Low non-haematological toxicity and accelerated haematopoietic recovery renders CEM with PBPC/growth factor support an acceptable therapeutic approach in an adjuvant or neoadjuvant setting.

Benedetti Panici, P.; Pierelli, L.; Scambia, G.; Foddai, M. L.; Salerno, M. G.; Menichella, G.; Vittori, M.; Maneschi, F.; Caracussi, U.; Serafini, R.; Leone, G.; Mancuso, S.

1997-01-01

100

Haematological malignancies: the rationale for integrated haematopathology services, key elements of organization and wider contribution to patient care.  

PubMed

The National Institute for Clinical Excellence (NICE) defined the process of care for haematological malignancies in Improving outcomes in haematological cancers. The manual in 2003. The most challenging recommendation has been the requirement to develop integrated laboratory services for accurate diagnosis. This is an aim that has not been fully achieved. The unified concept of haematological malignancy is recent and based on an understanding of the cellular pathology of the bone marrow and immune systems. Historical UK pathology practice has previously resulted in the separation of laboratory haematology from histopathology and of liquid and tissue specimens. Proposals for reintegration and centralization with specialist-led, centralized diagnostic and reporting services challenge the fragmented historical model. Accuracy and certainty of diagnosis remains problematic, particularly for lymphomas, with evidence that the accuracy of diagnosis is slowly improving but still only approaches 85%. There is a potentially significant human and financial cost of diagnostic errors. No nationwide, validated and comparable epidemiology/population-based data exist for accurate and complete ascertainment of new cases of haematological cancer, service planning or clinical outcomes monitoring. This article examines the original rationale behind the NICE guidance and outlines the key components and processes of an integrated diagnostic service. PMID:21261689

Ireland, Robin

2011-01-01

101

Evaluation of sulfonamide detoxification pathways in haematologic malignancy patients prior to intermittent trimethoprim-sulfamethoxazole prophylaxis  

PubMed Central

AIMS Patients with haematologic malignancies have a reportedly high incidence of sulfamethoxazole (SMX) hypersensitivity. The objective of this study was to determine whether deficiencies in sulfonamide detoxification pathways, to include glutathione (GSH) and ascorbate (AA), and cytochrome b5 (b5) and cytochrome b5 reductase (b5R), were prevalent in these patients. A secondary pilot objective was to determine whether the incidence of drug hypersensitivity following intermittent trimethoprim-SMX (TMP-SMX) prophylaxis approached that reported for high dose daily regimens. METHODS Forty adult patients with haematologic malignancies (HM) and 35 healthy adults were studied; an additional 13 HM patients taking ascorbate supplements (HM-AA) were also evaluated. Twenty-two of 40 HM patients were prescribed and were compliant with TMP-SMX 960 mg three to four times weekly. RESULTS There were no significant differences between HM and healthy groups in plasma AA (median 37.2 µmvs. 33.9 µm) or red blood cell GSH (1.9 mmvs. 1.8 mm). However, plasma AA was correlated significantly with leucocyte b5/b5R reduction (r = 0.39, P = 0.002). Deficient b5/b5R activities were not found in HM patients. In fact, patients with chronic lymphocytic leukaemia or myeloma had significantly higher median activities (80.7 µmol mg–1 min?1) than controls (18.9 µmol mg–1 min?1, P = 0.008). After 3–4 weeks of treatment, no patients developed SMX-specific T cells and only one patient developed rash. CONCLUSIONS Deficiencies of blood antioxidants and b5/b5R reduction were not found in this population with haematologic malignancies, and the development of skin rash and drug-specific T cells appeared to be uncommon with intermittent TMP-SMX prophylaxis.

Abouraya, Mahmoud; Sacco, James C; Kahl, Brad S; Trepanier, Lauren A

2011-01-01

102

Monitoring trough voriconazole plasma concentrations in haematological patients: real life multicentre experience.  

PubMed

The objective of this retrospective study was to evaluate results from voriconazole therapeutic drug monitoring (TDM) in haematological patients in routine clinical practice. Between 2005 and 2010, 1228 blood samples were obtained from 264 haematological patients (median 3 samples/patient; range 1-27) receiving voriconazole for targeted/preemptive treatment of invasive aspergillosis (IA) (46.3% of samples), empirical therapy (12.9%) or prophylaxis (40.8%). A high-pressure liquid chromatography assay was used to analyse voriconazole concentrations. Clinical and laboratory data were analysed retrospectively. The median of the detected voriconazole plasma concentration was 1.00 ?g ml(-1) (range <0.20-13.47 ?g ml(-1)). Significant inter- and intra-patients variability of measured concentrations (81.9% and 50.5%) were identified. With the exception of omeprazole administration, there was no relevant relationship between measured voriconazole concentrations and drug dose, route administration, age, gender, CYP2C19*2 genotype, gastrointestinal tract abnormality, administration via nasogastric tube, serum creatinine, and liver enzymes. However, per patient analysis identified significant role of individual voriconazole dose and drug form change on measured plasma concentration. Measured voriconazole concentrations did not correlate with the treatment outcome of patients with IA. We only identified a limited number of adverse events related to voriconazole therapy; however, the median plasma concentration was not different from concentrations measured in samples without reported toxicity. Our retrospective study has suggested that routine monitoring of voriconazole plasma concentrations has probably only a limited role in daily haematological practice. PMID:22429709

Racil, Zdenek; Winterova, Jana; Kouba, Michal; Zak, Pavel; Malaskova, Ludmila; Buresova, Lucie; Toskova, Martina; Lengerova, Martina; Kocmanova, Iva; Weinbergerova, Barbora; Timilsina, Shira; Rolencova, Monika; Cetkovsky, Petr; Mayer, Jiri

2012-03-19

103

Focus on invasive mucormycosis in paediatric haematology oncology patients: a series of 11 cases.  

PubMed

Mucormycosis has emerged as an increasingly important infection in oncology centres with high mortality, especially in severely immunocompromised patients. We carried out a retrospective study of 11 children with mucormycosis treated in seven French oncology-haematology paediatric wards during the period from 1991 to 2011. Lichtheimia corymbifera and Mucor spp. were the predominant pathogens. Treatment regimens included antifungal therapy, reversal of underlying predisposing risk factors and surgical debridement. Although mucormycosis is associated with high mortality, this infection could be cured in eight of our cases of severely immunocompromised paediatric cancer patients. PMID:23020159

Phulpin-Weibel, Aurélie; Rivier, Alexandre; Leblanc, Thierry; Bertrand, Yves; Chastagner, Pascal

2012-10-01

104

New aspects of the insulin resistance syndrome: impact on haematological parameters  

Microsoft Academic Search

.\\u000a Aim\\/hypothesis:   Previous studies have shown that insulin has an important in vitro role in the regulation of human erythropoiesis. We investigated\\u000a whether in vivo hyperinsulinaemia\\/insulin resistance affects haematological parameters. \\u000a \\u000a \\u000a \\u000a Methods:   A total of 608 subjects between 22 and 99 years of age were enrolled in the Chianti study, an epidemiological study of factors\\u000a affecting mobility in old age. The

M. Barbieri; E. Ragno; E. Benvenuti; G. A. Zito; A. Corsi; L. Ferrucci; G. Paolisso

2001-01-01

105

Disseminated mucormycosis in haematological patients: CT and MRI findings with pathological correlation.  

PubMed

Disseminated mucormycosis is a rare, mostly fatal infectious complication in immunocompromised haematological patients. The purpose of our study was to describe the multiorgan manifestations of disseminated mucormycosis documented at CT and MRI in four patients and correlate these with the pathological findings and patient outcome. Irrespective of the site of infection, infarction or haemorrhage are the constant features of invasive mycosis. Identification of one or both of these two major imaging findings in immunocompromised patients should be regarded as an indicator of possible infection by angiotropic fungi, including the genre Mucorales. PMID:16940368

Horger, M; Hebart, H; Schimmel, H; Vogel, M; Brodoefel, H; Oechsle, K; Hahn, U; Mittelbronn, M; Bethge, W; Claussen, C D

2006-09-01

106

Fifteenth biannual report of the Cochrane Haematological Malignancies Group--focus on non-Hodgkin's lymphoma.  

PubMed

This fifteenth biannual report of the Cochrane Haematological Malignancies Group (CHMG) highlights recently published randomized controlled trials (RCTs) in the field of hemato-oncology, covering the publication period from October 2011 to May 2012. Implications for clinical practice and methodological aspects are the main principles for selecting trials for this report. Studies were identified by electronic search of MEDLINE using a broad search filter that covers all topics in hemato-oncology combined with a highly sensitive search filter for randomized trials (Cochrane Handbook for Systematic Reviews of Interventions). PMID:23852951

Rancea, Michaela; Will, Andrea; Borchmann, Peter; Monsef, Ina; Engert, Andreas; Skoetz, Nicole

2013-07-12

107

Twelfth biannual report of the Cochrane Haematological Malignancies Group--focus on tyrosine kinase inhibitors.  

PubMed

This 12th biannual report of the Cochrane Haematological Malignancies Group highlights recently published randomized controlled trials in the field of hemato-oncology, covering the publication period from September 1, 2009, through June 30, 2010. Implication for clinical practice and methodological aspects are the main principles used to select trials for this report. Studies on tyrosine kinase inhibitors for patients with chronic myeloid leukemia were identified through electronic search of MEDLINE with a broad search filter that covered all topics in hemato-oncology combined with a highly sensitive search filter for randomized studies as described in the Cochrane Handbook for Systematic Reviews of Interventions. PMID:21252264

Skoetz, Nicole; Kluge, Sabine; Monsef, Ina; Bauer, Kathrin; Siebert, Hendrik; Kreuzer, Karl-Anton; Engert, Andreas

2011-01-20

108

Activity of cyclosporins as resistance modifiers in primary cultures of human haematological and solid tumours.  

PubMed Central

The semiautomated fluorimetric microculture cytotoxicity assay (FMCA) was used for evaluation of the ability of cyclosporin A (CsA) and its novel non-immunosuppressive derivative SDZ PSC 833 (PSC) to modify the response to doxorubicin or vincristine in vitro in different haematological and solid human tumour types. Primary cultures of 322 tumour samples were analysed. Both cyclosporins showed resistance-modifying activity in all haematological tumours tested, and in solid tumours activity was observed in ovarian carcinoma and childhood tumours. Little or no effect was found in the remaining tumour types, including breast, renal and adrenal cortical carcinomas and adult sarcomas. In most of the responsive cases the interaction between the modifier and the cytotoxic drug was synergistic. There was a tendency to higher activity in samples from previously treated patients, and an inverse relationship between degree of cytotoxic drug resistance and resistance-modifying activity was noted. No difference in potency between CsA and PSC could be discerned. The results indicate differential in vitro resistance-modifying activity of the cyclosporins depending on tumour type. The results also suggest that treatment with resistance modifiers should be considered also for primary therapy of drug-sensitive tumours. Drug resistance assays such as the FMCA may become useful in preclinical evaluation of resistance modifiers.

Fridborg, H.; Jonsson, B.; Nygren, P.; Csoka, K.; Nilsson, K.; Oberg, G.; Kristensen, J.; Bergh, J.; Tholander, B.; Olsen, L.

1994-01-01

109

Guideline on the prevention of secondary central nervous system lymphoma: British Committee for Standards in Haematology.  

PubMed

The guideline group was selected to be representative of UK-based medical experts. Ovid MEDLINE, EMBASE and NCBI Pubmed were searched systematically for publications in English from 1980 to 2012 using the MeSH subheading 'lymphoma, CNS', 'lymphoma, central nervous system', 'lymphoma, high grade', 'lymphoma, Burkitt's', 'lymphoma, lymphoblastic' and 'lymphoma, diffuse large B cell' as keywords, as well as all subheadings. The writing group produced the draft guideline, which was subsequently revised by consensus by members of the Haemato-oncology Task Force of the British Committee for Standards in Haematology (BCSH). The guideline was then reviewed by a sounding board of ~50 UK haematologists, the BCSH and the British Society for Haematology (BSH) Committee and comments incorporated where appropriate. The 'GRADE' system was used to quote levels and grades of evidence, details of which can be found in Appendix I. The objective of this guideline is to provide healthcare professionals with clear guidance on the optimal prevention of secondary central nervous system (CNS) lymphoma. The guidance may not be appropriate to patients of all lymphoma sub-types and in all cases individual patient circumstances may dictate an alternative approach. Acronyms are defined at time of first use. PMID:24033102

McMillan, Andrew; Ardeshna, Kirit M; Cwynarski, Kate; Lyttelton, Matthew; McKay, Pam; Montoto, Silvia

2013-08-27

110

Haematological acclimation and re-acclimation to hypoxia in the mouse.  

PubMed

Haematological responses throughout 4w of initial acclimation (IA) and three paradigms of re-acclimation (RA) to hypoxia ( [Formula: see text] ) were examined in female mice. We hypothesised that (i) haematological responses would be increased during re-exposure, resulting in greater O2-carrying capacity in RA compared to IA; and (ii) further improvements would occur when abbreviating the de-acclimation period to 1w (RA?DA) or extending the IA period to 8w (RA?IA). The serum [EPO] response was blunted in all RA groups compared to IA but the resulting reticulocyte response was similar in all experimental groups. The [Hb] response was the same in RA and RA?DA as in IA but was blunted in RA?IA due to a reduction in mean corpuscular Hb. The sensitivity of EPO-producing cells appears blunted but the sensitivity of erythroid precursors to EPO is enhanced by recent hypoxic exposure. Erythropoietic regulation is altered during RA in a manner that is dependent on the paradigm of initial exposure. PMID:23891710

Macnutt, Meaghan J; Rupert, James L; Sheel, A William

2013-07-24

111

Haematological and blood biochemical studies in female domesticated Indian elephants (Elaphas maximus L.).  

PubMed

1. Some haematological and biochemical blood parameters in female Indian elephants were investigated. 2. Haematological data were as follows: Ht = 39.2 +/- 2.36%, Hb = 10.1 +/- 0.54 g%, RBC = 2.66 +/- 0.32 x 10(6)/mm3, WBC = 5.43 +/- 0.48 x 10(3)/mm3. Lymphocytes, determined on blood smears were mainly leucocytes from (67.0 +/- 1.59%). Data for MCV, MCH and MCHC are also given. 3. Blood plasma was separated into 5 main fractions, total plasma protein concentration was 6.98 +/- 0.53 g%, A/G ratio was 0.69 +/- 0.1. 4. Plasma minerals concentration was as follows: Na, 3044 +/- 194 micrograms/ml; K, 529 +/- 38.5 micrograms/ml; Mg, 33.0 +/- 3.43 micrograms/ml; Ca, 181.0 +/- 17.8 micrograms/ml; InP, 44.6 +/- 6.1 micrograms/ml. Ca: P ratio was 3.25 +/- 0.34. 5. Some seasonal differences in investigated parameters were observed. Ht values, WBC and neutrophils number as well as Ca, and Mg concentrations were higher during winter, whereas RBC and Na and InP concentrations were lower in winter. PMID:2896562

Gromadzka-Ostrowska, J; Jakubów, K; Zalewska, B; Krzywicki, Z

1988-01-01

112

Cell counting of body fluids: comparison between three automated haematology analysers and the manual microscope method.  

PubMed

INTRODUCTION: Haematological analysis of body fluids (BF) specimens can provide clinicians with valuable diagnostic information because it can indicate one of several serious medical conditions. Although up to now the microscopic counting and the differentiation of WBC in a BF smear have been used as a reference. The introduction of semiautomated and automated methods of analysis has reduced interoperator variability and improved turnaround time and precision. The aim of our study was to evaluate the accuracy and the correlation between the three methods and with the reference method. METHODS: We examined 110 body fluid samples. Total counting of each sample has been conducted with all systems: Pentra DX120, ADVIA 2120 and XE-2100 and the manual method. RESULTS: We found statistically significant correlation between the data obtained in the ascitic and pleuric liquid but not in the cerebrospinal fluid. CONCLUSION: The introduction of automated method for BF analysis is more and more useful in the routine job of a laboratory analysis. It is therefore very important to evaluate the performance of the different automated haematology technologies, because there is a lack of literature in this field. The comparison between the Pentra DX 120, the other technologies and the manual counting showed instrumental overlapping capabilities. PMID:23647736

Danise, P; Maconi, M; Rovetti, A; Avino, D; Di Palma, A; Gerardo Pirofalo, M; Esposito, C

2013-05-01

113

Haematological effects among silk screening workers exposed to 2-ethoxy ethyl acetate  

PubMed Central

Aims: To investigate the haematological effects in 2-EEA exposed workers. Methods: Workers from one silk screening shop (n = 29), using 2-EEA as the major cleaning and printing solvent, were recruited as a high exposure group. Workers with indirect and non-exposure to 2-EEA (n = 56) were recruited as the comparison group. Venous blood was collected for blood routine examination. Air concentration of 2-EEA in this plant was measured by eight hour personal sampling. Results: The geometric mean (GM) of air concentration of 2-EEA in the high exposure group was 7.41 ppm (range 1.35–16.5 pppm). The mean exposure of female workers (GM = 9.34 ppm) was significantly higher than that of male workers (GM = 4.87 ppm). The GM of air 2-EEA concentration in the comparison group was 0.07 ppm (range: non-detectable to 3.62 ppm, n = 26). The haemoglobin and haematocrit in the female high 2-EEA exposure workers were significantly lower than those of female workers in the comparison group. No difference was found between male 2-EEA high exposure and comparison group workers. The haemoglobin, haematocrit, and RBC count in the study population had a significant dose-response relation with air 2-EEA levels. Conclusion: Results suggest that 2-EEA is a haematological toxicant, which leads to anaemic status in high exposure female workers.

Loh, C; Shih, T; Liou, S; Lin, Y; Hsieh, A; Chen, C; Liao, G

2003-01-01

114

Prospective study of hepatic, renal, and haematological surveillance in hazardous materials firefighters  

PubMed Central

OBJECTIVES—To evaluate possible health effects related to work with hazardous materials as measured by end organ effect markers in a large cohort over about 2 years, and in a subcohort over 5 years.?METHODS—Hepatic, renal, and haematological variables were analysed from 1996-98 in hazardous materials firefighters including 288 hazardous materials technicians (81%) and 68 support workers (19%). The same end organ effect markers in a subcohort of the technicians were also analysed (n=35) from 1993-98. Support workers were considered as controls because they are also firefighters, but had a low potential exposure to hazardous materials.?RESULTS—During the study period, no serious injuries or exposures were reported. For the end organ effect markers studied, no significant differences were found between technicians and support workers at either year 1 or year 3. After adjustment for a change in laboratory, no significant longitudinal changes were found within groups for any of the markers except for creatinine which decreased for both technicians (p<0.001) and controls (p<0.01).?CONCLUSIONS—Health effects related to work are infrequent among hazardous materials technicians. Haematological, hepatic, and renal testing is not required on an annual basis and has limited use in detecting health effects in hazardous materials technicians.???Keywords: hazardous materials; firefighters; medical surveillance

Kales, S; Polyhronopoulos, G; Aldrich, J; Mendoza, P; Suh, J; Christiani, D

2001-01-01

115

Patients with haematological malignancies requiring invasive mechanical ventilation: differences between survivors and non-survivors in intensive care unit  

Microsoft Academic Search

Background: Mortality of patients with haematological malig- nancies requiring intensive therapy is high. We wanted to establish reasons for intensive care unit (ICU) admis- sion and treatment as well as outcome in subjects who required invasive mechanical ventilation. We were also interested in differences between ICU survivors and non-survivors at the moment of admission. Patients and methods: Forty patients (21

Radoslaw Owczuk; Magdalena A. Wujtewicz; Wioletta Sawicka; Anna Wadrzyk; Maria Wujtewicz

2005-01-01

116

Morphological and haematological parameters in the Algerian mouse ( Mus spretus) inhabiting an area contaminated with heavy metals  

Microsoft Academic Search

In the present study morphological (ratio of body weight to body length, weight of internal organs) and haematological parameters (cells count, haemoglobin concentration, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration) were used for monitoring potential effects of metal contaminants on the Algerian mouse (Mus spretus). The study also provided the opportunity to evaluate the use of

A. C Nunes; M da Luz Mathias; A. M Crespo

2001-01-01

117

The investigation of parvovirus B19 infection in patients with haematological disorders by using PCR and ELISA techniques  

Microsoft Academic Search

Parvovirus B19 has a marked tropism for erythroid progenitor cells. This may lead to chronic anemia in predisposed individuals. The purpose of the study was to investigate the frequency of parvovirus B19 infections in patients with diagnosis of haematological disorders. In order to determine the diagnostic use of different markers of parvovirus B19 infection, serum specimens obtained from 79 patients

Tercan Us; Leyla Ozunel; Nilgun Kasifoglu; Yurdanur Akgun

2007-01-01

118

The control of hyperendemic glycopeptide-resistant Enterococcus spp. on a haematology unit by changing antibiotic usage  

Microsoft Academic Search

The rectal carriage of glycopeptide-resistant Enterococcus spp. (GRE) had been established at approximately 50% in a series of prevalence studies on a busy haematological malignancy unit. The aim of this study was to reduce the chance of patients acquiring GRE. A prospective three- phase sequential study was performed. In Phase 1, the acquisition rate of GRE detectable by rectal swab

Susan J. Bradley; Angela L. T. Wilson; Michael C. Allen; Hilary A. Sher; Anthony H. Goldstone; Geoffrey M. Scott

1999-01-01

119

Integrating a Primer Course in Biostatistics into the Haematology Practicals of First-Year Medical Students in India  

Microsoft Academic Search

Context: In India, biostatistics in the medical curriculum is usually taught as a separate course, using either ``imaginary'' data or data from the literature. Knowledge of statistics is particularly important in the context of ``evidence-based medicine''. Objective: To evaluate the ef® cacy of a biostatistics course integrated into the practical haematology ® rst-year medical course with the following special characteristics:

Mario Vaz

2001-01-01

120

Some Biochemical, Haematological and Histological Responses to a Long Term Consumption of Telfairia occidentalis-Supplemented Diet in Rats  

Microsoft Academic Search

2 Abstract: Some biochemical, haematological and histological responses were studied in rats undergoing a long term feeding with a Telfairia occidentalis-supplemented diet. Biochemical and hematological parameters investigated included serum protein, total cholesterol, lipid peroxidation, haemoglobin, white blood cells, Aspartate aminotransferase, Alanine aminotransferase, Alkaline phosphatase, Glutathione-s- transferase and Superoxide dismutase. Histological changes associated with Telfairia occidentalis- supplemented diet on the liver,

Onyechi Obidoa

2009-01-01

121

Comparison of the clinical, microbiological, radiological and haematological features of foals with pneumonia caused by Rhodococcus equi and other bacteria  

Microsoft Academic Search

The purpose of this study was to compare the clinical, microbiological, radiological, haematological and cytological features of foals with pneumonia caused by Rhodococcus equi infection and with other bacteria, in order to provide markers for early diagnosis and treatment. A retrospective study of 113 cases of bacterial pneumonia was undertaken.Although there was considerable overlap in the affected populations, foals with

Mathilde Leclere; K. Gary Magdesian; Philip H. Kass; Nicola Pusterla; Diane M. Rhodes

2011-01-01

122

The relationship between cardiac and liver iron evaluated by MR imaging in haematological malignancies and chronic liver disease  

PubMed Central

Although iron overload is clinically significant, only limited data have been published on iron overload in haematological diseases. We investigated cardiac and liver iron accumulation by magnetic resonance imaging (MRI) in a cohort of 87 subjects who did not receive chelation, including 59 haematological patients. M-HIC (MRI-based hepatic iron concentration, normal values <36??mol/g) is a non-invasive, liver biopsy-calibrated method to analyse iron concentration. This method, calibrated to R2 (transverse relaxation rate), was used as a reference standard (M-HIC(R2)). Transfusions and ferritin were evaluated. Mean M-HIC(R2) and cardiac R* of all patients were 142??mol/g (95% CI, 114–170) and 36.4?1/s (95% CI, 34.2–38.5), respectively. M-HIC(R2) was higher in haematological patients than in patients with chronic liver disease or normal controls (P<0.001). Clearly elevated cardiac R2* was found in two myelodysplastic syndrome (MDS) patients with severe liver iron overload. A poor correlation was found between liver and cardiac iron (n=82, r=0.322, P=0.003), in contrast to a stronger correlation in MDS (n=7, r=0.905, P=0.005). In addition to transfusions, MDS seemed to be an independent factor in iron accumulation. In conclusion, the risk for cardiac iron overload in haematological diseases other than MDS is very low, despite the frequently found liver iron overload.

Virtanen, J M; Remes, K J; Itala-Remes, M A; Saunavaara, J P; Komu, M E; Partanen, A M; Parkkola, R K

2012-01-01

123

Malaria and haematologic parameters of pupils at different altitudes along the slope of Mount Cameroon: a cross-sectional study  

PubMed Central

Background Haematologic abnormalities are features in Plasmodium falciparum infection, and anaemia is an inevitable outcome. This study examines the influence of malaria status and altitude on haematologic parameters in school-aged pupils. Methods A cross-sectional study was conducted among 728 school pupils aged between four and 15 years at three different altitudinal ranges along the slope of the Mount Cameroon region. The investigative methods included the use of questionnaire, clinical evaluation and laboratory investigations. Blood sample collected from each child was used for the preparation of blood films for detection of malaria parasites and assessment of malaria parasite density as well as full blood count determination using an automated haematology analyzer. Results The prevalence of malaria in the study population was 33.8% and 64.2% (158/246) of these were asymptomatic (AM). Pupils in lowlands had a significantly (P <0.05) prevalence (95% confidence interval, CI) of malaria (60.6%, CI?=?54.6–65.9%) than those in middle belt (29.1%, CI?=?23.9–34.8%) and highlands (7.7%, CI?=?6.1–9.8%), while those in middle belt had significantly higher geometric mean parasite density (475) than those in lowlands (233) and highlands (388). The prevalence of malaria was significantly higher in children that presented with fever (40.4%, CI?=?33.8–47.2%) when compared with afebrile subjects (31%, CI?=?27–35.2%). Pupils with AM had a higher prevalence of leucopaenia (43.7%, CI?=?35.8–51.8%), microcytosis (27.2%, CI?=?20.5–34.9%), hypochromasia (27.8%, CI?=?21–35.5%) and thrombocytopaenia (14.9%, CI?=?8.9–22.8%) when compared with those with clinical malaria (CM). All mean haematological parameters were comparable in pupils with CM and AM, except for the mean white blood cell (WBC) counts. Pupils with AM had significantly lower (P?=?0.02) mean WBC counts (5.1?±?2.5?×?109/L) than those with CM (5.9?±?2.3?×?109/L). Age, altitude and malaria parasitaemia was of significant influence on several haematological parameters. Conclusion Altitude influenced the distribution and density of malaria parasites and was of confounding influence on the haematologic profiles. These results highlight the insidious effects of AM on the haematologic components.

2013-01-01

124

Clinical, haematological and therapeutic studies on tropical theileriosis in water buffaloes (Bubalus bubalis) in Egypt.  

PubMed

Thirty buffaloes naturally infected with Theileria annulata and 10 parasitologically free controls were used to determine the potential clinical, haematological and therapeutic impact of tropical theileriosis in Egypt. The clinical signs in the infected buffaloes were pyrexia (40.5-41.5 degrees C), enlargement of superficial lymph nodes, slight nasal and ocular discharges, salivation, anaemia and respiratory distress. Eye lesions also were recorded. There was a significant decrease in erythrocyte counts and haemoglobin content and a significant decrease in total leucocyte counts in infected buffaloes compared to controls. Early treatment with buparvaquone was 100% effective in eliminating the protozoan parasites from the blood and lymph nodes and led to an improvement in the clinical state whereas treatment in the later stages of the disease whilst eliminating the parasites failed to improve the clinical condition of the animal. PMID:17420101

Osman, Salama A; Al-Gaabary, Magdy H

2007-04-08

125

Effects of Aflatoxin on Some Haematological Parameters and Protective Effectiveness of Esterified Glucomannan in Merino Rams  

PubMed Central

The objective of the present study was to evaluate the toxic effects of aflatoxin on some hematological parameters and to determine the preventive effectiveness of added glucomannan. In the study, 32 Merino rams were used, and the rams were separated equally to four groups as control (C), glucomannan (G), glucomannan?+?aflatoxin (AG), and aflatoxin (A). Erythrocyte, leukocyte count, hemoglobin, and hematocrit levels were decreased in A group compared with the other groups, and there was a reduction in similar parameters in AG group compared to control values. On the other hand, these parameters were tended to increase in AG group compared to A group values. Aflatoxicosis caused the lymphocytopenia and monocytopenia but increased percentage of neutrophil counts. In conclusion, the results determined in the study might be important to demonstrate the effects of aflatoxicosis and glucomannan on some haematological parameters before the clinical symptoms appear.

Donmez, Nurcan; Donmez, H. H.; Keskin, E.; K?sadere, I.

2012-01-01

126

Analysis of some biochemical and haematological parameters for Mucuna pruriens (DC) seed powder in male rats.  

PubMed

The biochemical and haematological effects of the seed powder of Mucuna pruriens in male rats were evaluated to establish some biological properties of this potential biopesticide currently undergoing investigation. The result showed that Mucuna pruriens seed extract produced a significant (p<0.05) increase in white blood cell (WBC) count, as well as in bilirubin concentrations, alkaline phosphatase (ALP), protein and creatinine levels measured. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly reduced (P<0.05) in comparison with the experimental control. PCV, Hb, albumin level and WBC differential counts gave no significant difference between treated and control groups. The results revealed metabolic imbalance in the rats which suggests a mild cholestasis effect of the extract. PMID:21959815

Chukwudi, Ndukwe Henry; Simeon, Omale; Chinyere, Aguiyi John

2011-10-01

127

Sickle cell disease in Sierra Leone: a clinical and haematological analysis in older children and adults.  

PubMed

A prospective study of the clinical and haematological features of sickle cell disease in 121 Sierra Leonean patients (106 SS: sickle cell anaemia; 15 SC: sickle cell haemoglobin C disease) was undertaken. Fifty-five per cent of the patients were Creoles who were descendants of liberated slaves and who therefore had strong ethnic and genetic relationships with West Indians and black Americans. No essential differences were noted between the Creoles and non-Creole Sierra Leonean patients. The general clinical features and the patterns of vaso-occlusive crises were similar to those of black American, Jamaican and other West Africans (Ghanaians and Nigerians). However the incidence of certain complications such as avascular necrosis of the femoral head was higher in Sierra Leoneans (8.5% SS, 20% SC) than in other West Africans e.g. Ghanaians (2.8% SS, 6.6% SC) but similar to that of Jamaicans (8.2% SS, 18% SC). Also the frequency of leg ulceration in Sierra Leonean sickle cell patients (13.2% SS, 13.3% SC) was relatively low when compared to patients in the United States of America (73.6% SS) and Jamaica (63.3% SS) but relatively high in comparison to Nigerian (5.4% SS + SC + S beta-thal) and Ghanaian (10.6% SS, 2.2% SC) patients. The causes for such varying manifestations are probably environmental. The haematological features of the Sierra Leonean patients were similar to those of other population groups in all essential aspects. PMID:6639186

Knox-Macaulay, H H

1983-08-01

128

Exploring haematology nurses' perceptions of specialist education's contribution to care delivery and the development of expertise.  

PubMed

The role that educational preparation may play in the delivery of care and the development of expertise is a point of some debate [Manley, K., Garbett, R., 2000. Paying Peter and Paul reconciling concepts of expertise with competency for a clinical career structure. Journal of Clinical Nursing 9 (3), 347; King, L., Macleod Clark, J., 2002. Intuition and the development of expertise in surgical ward and intensive care nurses. Journal of Advanced Nursing 37(4), 322-329; Bonner, A., 2003. Recognition of expertise: an important concept in the acquisition of nephrology nursing expertise. Nursing & Health Sciences Journal 5, 123-131; Dunphy, B.C., Williamson, S.L., 2004. In pursuit of expertise. Advances in Health Sciences Education 9, 107-127]. Though education is a concept that may be universally valued, it may be more difficult to clearly discern the significance it has for practitioners who are developing their expertise. This research project employed an interpretive phenomenological design to explore the perceptions of specialist haematology nursing staff on the extent to which specialist education contributes to care delivery and the development of expert practice. A non-representative purposive sample of qualified nurses who had undertaken specialist education in haemopoiesis and work in specialist haematology participated in a focus group and semi-structured interviews. The report concludes that, for these specialist practitioners, specialist educational input had a beneficial impact on their levels of knowledge and confidence. Further to this, involvement in higher education had enabled them to become more active in the learning process. Perhaps the key finding of the study was the assertion by respondents that specialist educational input had enabled them to develop their specialist practice to a level that experience alone could not achieve. PMID:17126955

Atkinson, Joanne; Tawse, Stephen

2006-11-28

129

The effects of probiotic and mannanoligosaccharide on some haematological and immunological parameters in turkeys.  

PubMed

The study was conducted to investigate the effects of mannanoligosaccharide (MOS) and probiotic supplementation on haematological and immunological parameters in turkeys. A total of 72, 15-day-old white hybrid converter turkey poults were used in this study. Poults were assigned into three groups, each group consisted of six poults and the trial was repeated four times. The control group was fed a basal diet without supplemented probiotic and MOS, and treatment groups were fed either 1 g/kg MOS or probiotic added diets. At the end of the 15-week treatment period, samples of blood were collected to determine immunological and haematological values. The comparison among the groups showed that both the probiotic and MOS supplementation resulted in significant increases (P < 0.05) in the serum IgG and IgM levels, and significant decreases (P < 0.05) in the peripheral blood T lymphocyte percentage compared with those of the control group. Mean serum IgG level (7.06 g/l) was significantly higher (P < 0.05) in poults supplemented with probiotic compared with MOS (6.76 g/l). It was observed that the probiotic supplementation caused statistically significant increases (P < 0.05) in the erythrocyte count, haemoglobin concentration and haematocrit values, but MOS supplementation did not have a significant effect (P > 0.05) on these parameters. Total leucocyte and differential leucocyte counts were not affected by dietary MOS and probiotic supplementation. These results show that MOS or probiotic may elevate IgG and IgM levels in turkey. The MOS and probiotic that enhance immunoglobulin levels will have a more positive effect on growth performance, production and turkeys' ability to resist disease. PMID:16050905

Cetin, N; Güçlü, B K; Cetin, E

2005-08-01

130

Platelet count evaluation using three automated haematology analysers compared with the immunoplatelet reference method, and estimation of possible inadequate platelet transfusion.  

PubMed

The accuracy of three automated haematology analysers [Sysmex XE-2100 (both optical and impedance mode), Bayer Advia 120, and Beckman Coulter LH-750] was compared with the immunoplatelet reference method for platelet measurement. A total of 165 blood specimens were obtained from patients and platelet counts were determined using the four-automated haematology analyser methods and the immunoplatelet reference method. The coefficients of determination (R(2)) between the automated haematology analyser methods and the immunoplatelet reference method for the overall platelet range were >0.98. A bias study, however, showed some disagreement. The use of a coincidence correction calculation for the immunoplatelet method did not improve the correlation between the immunoplatelet method and the automated haematology analyser methods. To estimate the possibility of inadequate platelet transfusion, the number of prophylactic platelet transfusion indications determined by the automated haematology analyser platelet counts were compared with the number of transfusion indications according to the platelet counts determined by the immunoplatelet method. An additional 48 blood specimens were included in this analysis. All of the automated haematology analysers showed some disagreement in the transfusion indications when compared with the immunoplatelet method, suggesting the possibility of inadequate platelet transfusion. PMID:18294237

Hong, K H; Kim, M J; Lee, K W; Park, K U; Kim, H S; Song, J

2008-02-21

131

The Effect of Cement Dust Exposure on Haematological Parameters of Cement Factory workers in Nalagonda, Andhra Pradesh.  

NASA Astrophysics Data System (ADS)

This study was measured on haematological parameters in workers exposed to cement dust in order to test the the hypothesis and to identify a simple, readily available, cost effective screening test that could help in identifying the presence of disease, its severity, that Cement dust exposure may perturb these functions related to their workplace.Assesment of haematological parameters were performed in 100exposed workers occupationally exposed to cement dust and 50 matched unexposed controls with ages ranging from 20-35, 35-50, 50-65 years. The blood samples were taken from them and percentage of hemoglobin, Lymphocytes / monocytes count were analysed.The hemoglobin percentage of exposed workers were significantly lower(P<0.05).Lymphocytes/Monocytes counts of exposed workers was insignificant (P<0.05).These results suggest that long term occupational exposure to cement dust may perturb haemopoietic function.

Guguloth, Mohan Rao.; Sambanaik, A.; srinivasnaik, L.; Mude, Jagadishnaik.

2012-10-01

132

Effect of 12-hour road transportation on physiological, immunological and haematological parameters in bulls housed at different space allowances  

Microsoft Academic Search

The effects of transporting Holstein Friesian bulls (n=72; bodyweight 403±3.5kg) for 12h by road were examined. Adrenal, haematological and immune responses, body temperature and performance were recorded. The animals had been previously housed for 96 days at three space allowances (1.2, 2.7 or 4.2m2 per bull). The bulls were allocated to one of two treatments: T (transport for 12h; n=16

S. Gupta; B. Earley; M. A. Crowe

2007-01-01

133

Nitrite toxicity in Cirrhinus mrigala (Ham.): acute toxicity and sub-lethal effect on selected haematological parameters  

Microsoft Academic Search

The acute toxicity and primary stress effect of the sub-lethal concentrations of nitrite (NO2?) on certain haematological parameters were studied in static bioassay systems in fingerlings of mrigal, Cirrhinus mrigala (Ham.), a species of major importance for aquaculture in India. The 96-h LC50 was 10.4 mg l?1. In sub-lethal test, the changes in the total erythrocyte count (TEC), total leukocyte

P. C. Das; S. Ayyappan; J. K. Jena; B. K. Das

2004-01-01

134

Growth performance, haematology and serum biochemistry of African catfish ( Clarias gariepinus ) fingerlings fed graded levels of dietary fumonisin B 1  

Microsoft Academic Search

Fingerlings of Clarias gariepinus were used to evaluate the effect of dietary fumonisin B1 (FB1), a mycotoxin produced by Fusarium verticillioides, on growth, haematological and serum biochemical parameters. The fingerlings were sorted, weighed and randomly stocked in\\u000a 16 plastic tanks at the rate of 20 fingerlings per tank. Fusarium-cultured maize grains containing FB1 were used to formulate three diets containing

Francis A. Gbore; Adeyemo M. Adewole; Olatunde Oginni; Mercy F. Oguntolu; Ayodeji M. Bada; Olatunbosun Akele

2010-01-01

135

Effects of ethanolic extracts of Garcinia kola seeds on growth and haematology of catfish (Clarias gariepinus) broodstock  

Microsoft Academic Search

A 56 day study was undertaken to evaluate the effects of dietary ethanolic extracts of Garcinia kola (Bitter kola) in African catfish, Clarias gariepinus broodstock on growth performance and basic haematological indices. Catfish broodstock (mean weight, 245.20 - 255.00 g) were randomly distributed into concrete tanks (2 x 2 x 1.2 m) at 10 fish\\/tank in triplicate treatments. 5 diets

A. A. Dada; M. Ikuerowo

136

Haematological and Biochemical Changes in Japanese Quails Coturnix coturnix Japonica and Chickens Due to Ascaridia galli Infection  

Microsoft Academic Search

2 Abstract: A study was carried out in Japanese quails Coturnix coturnix japonica and chickens (Rhode Island Red) for comparative haematological and biochemical changes occurring due to Ascaridia galli infection. Quails as well as chickens were divided into four small groups, each consisting six numbers of birds in each group and were marked as q1, q2, q3, qC and p1,

K. Deka; J. Borah

2008-01-01

137

A randomised, prospective comparison of allogeneic bone marrow and peripheral blood progenitor cell transplantation in the treatment of haematological malignancies  

Microsoft Academic Search

We present the results of a prospective, randomised study comparing PBPC and BM focusing on engraftment, acute and chronic GVHD and survival. Forty patients with haematological malignancies received HLA-identical sibling BM (group A) or PBPC (group B). Evaluable patients were 19 (A) and 18 (B). Median age was 35 (17–56) in A and 29.5 (9–51) in B. Conditioning was mainly

AC Vigorito; WM Azevedo; JFC Marques; AM Azevedo; KAB Eid; FJP Aranha; I Lorand-Metze; GB Oliveira; MEP Correa; ARC Reis; ECM Miranda; CA de Souza

1998-01-01

138

Role of Haematological Parameters as an Indicator of Acute Malarial Infection in Uttarakhand State of India  

PubMed Central

Background Malaria may be associated with complications which may be avoided by early diagnosis and treatment. Microscopic diagnosis showing presence of malarial parasites is needed for confirmation which at times may be unreliable and requires technical expertise. The present study was conducted to statistically analyze the haematological parameters including platelet indices which can give initial hint for malarial infection and therefore prompt the laboratory physician for active search of the parasite microscopically. Methods A retrospective study was conducted which included 334 cases of acute malaria caused by Plasmodium vivax, falciparum and dual infection along with 100 cases of control. Routine haematological parameters along with platelet indices (MPV and PDW) which are easily available on automated cell counter were statistically analyzed to assess their role as indicators for malaria. Results Leukocyte count and platelet count were significantly lower in cases of acute malaria in comparison to controls (p value <0.001). Platelet count<150×109/l showed 87.2% sensitivity, 65% specificity, 89.3% positive predictive value and 2.49 likelihood ratio for the infection. PDW of 6–10 and MPV>8 fl had 71.9% and 61.5% sensitivity and 78.2% and 77.7% positive predictive value respectively for infection. Platelet count <150×109/l and MPV>8 fl was comparatively more sensitive indicator for vivax (88% and 70.8% respectively) than falciparum (84.8% and 50.4% respectively) and PDW 6–10 was more sensitive indicator for falciparum (82.6%) than vivax (69.5%) infection. Conclusion Thrombocytopenia (<150×109/l) and low leukocyte count (<4×109/l) may be used as probable indicator for malaria in endemic countries. Higher MPV (>8 fl) and PDW of 6–10 also show considerable sensitivity for malarial infection. In addition, thrombocytopenia (<150×109/l) and higher MPV (>8 fl) was more sensitive for vivax infection while PDW 6–10 was more sensitive for falciparum infection.

Chandra, Smita; Chandra, Harish

2013-01-01

139

Outbreak of Multi-Drug Resistant Pseudomonas aeruginosa Bloodstream Infection in the Haematology Unit of a South African Academic Hospital  

PubMed Central

Objective To describe an outbreak of multi-resistant Pseudomonas aeruginosa bloodstream infections (MRPA-BSI) that occurred in the haematology ward of a tertiary academic hospital in Cape Town, South Africa, and determine risk factors for acquisition of MRPA-BSI. Methods The outbreak investigation included a search for additional cases, review of patient records, environmental and staff screening, molecular typing using pulsed-field gel electrophoresis (PFGE) and Multi-locus sequencing (MLST) and a retrospective case-control study. Results Ten MRPA-BSI cases occurred in the haematology ward between January 2010 and January 2011. The case fatality rate was 80%. Staff screening specimens were negative for MRPA and an environmental source was not identified. PFGE showed that 9/10 isolates were related. MLST showed that 3 of these 9 isolates belonged to Sequence type (ST) 233 while the unrelated isolate belonged to ST260. Conclusion We have described an outbreak of MRPA-BSI occurring over an extended period of time among neutropenic haematology patients. Molecular typing confirms that the outbreak was predominantly due to a single strain. The source of the outbreak was not identified, but the outbreak appears to have been controlled following intensive infection control measures.

Mudau, Maanda; Jacobson, Rachael; Minenza, Nadia; Kuonza, Lazarus; Morris, Vida; Engelbrecht, Heather; Nicol, Mark P.; Bamford, Colleen

2013-01-01

140

Effect of Aqueous Extract of Telfairia occidentalis Leaf on the Performance and Haematological Indices of Starter Broilers  

PubMed Central

This experiment was conducted to determine the effect of aqueous extract of Telfairia occidentalis (Fluted Pumpkin) leaf on the performance and haematological indices of starter broilers. A total of 200, 8-day-old broiler chicks were randomly allotted to five (5) treatments, each with 4 replicate groups containing 10 chicks and fed with standard starter broiler diets. Telfaria occidentalis leaves extract (FPLE) was added at 0, 40, 80, 120, and 160?mL/litre of drinking water. Growth performance and haematological indices were evaluated. Results showed that there was significant (P < 0.05) difference in weight gain, feed conversion, and protein efficiency ratios of the birds among the treatments. Birds fed 80?ml FPLE/litre of water had significantly the highest weight gain and the best feed conversion and protein efficiency ratios. There was no significant (P > 0.05) variations in the feed and water intakes of the birds. Results also show no significant (P > 0.05) difference in haematological indices of birds among the treatments. The results of this study indicate that, for enhanced weight gain and feed conversion efficiency, birds should be fed 80?mL FPLE/litre of water.

P. N., Onu

2012-01-01

141

Effect of azadirachtin on haematological and biochemical parameters of Argulus-infested goldfish Carassius auratus (Linn. 1758).  

PubMed

Argulosis hampers aquaculture production and alters the host physiology and growth. Azadirachtin is recognized as a potential antiparasitic agent against Argulus sp. The present study aimed to investigate the effect of different concentration of azadirachtin solution on haematological and serum biochemical parameters of Argulus-infested goldfish Carassius auratus. Ninety Argulus-infested goldfish were randomly divided into six equal groups. Fish of group 1-5 were treated with azadirachtin solution through bath of 1, 5, 10, 15 and 20 mg L(-1) as T1, T2, T3, T4 and T5, respectively, and group 6 was exposed to 2% DMSO solution without azadirachtin and considered as negative control T0(-). Along with six treatment groups, a positive control T0(+) of healthy goldfish free from Argulus infestation was also maintained. Parasitic mortality was evaluated after 3 days of consecutive bath treatment. After 7 days of post-treatment, the blood and serum were drawn from each of the treatment groups and haematological and serum biochemical parameters were evaluated. Total leucocyte count (TLC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), blood glucose, total protein (TP), globulin, serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) were significantly (p < 0.05) high in negative control group when compared with positive control group. It could be concluded that Argulus infestation altered marked haematological and serum biochemical parameters. However, in treated groups complete elimination of Argulus was found in T4 and T5 groups. Also significant (p < 0.05) reduction in haematological and serum biochemical parameters of all the treatment groups were recorded in comparison with negative control group. In addition, T4 and T5 groups showed significantly (p < 0.05) high superoxide dismutase (SOD), catalase, total erythrocyte count (TEC) and haemoglobin (Hb). However, higher mean corpuscular haemoglobin concentration (MCHC), blood glucose and lactate dehydrogenase (LDH) levels in T5 group revealed that higher concentration of azadirachtin have notable effects on activity of vital tissues function and physiology of the host. Argulus spp. from infested goldfish could be eliminated using bath treatment with solution of azadirachtin having concentration of 15 mg L(-1) and that also shifted haematological and serum biochemical parameters towards homeostasis. PMID:23090629

Kumar, Saurav; Raman, R P; Kumar, Kundan; Pandey, P K; Kumar, Neeraj; Mallesh, B; Mohanty, Snatashree; Kumar, Abhay

2012-10-23

142

Assessment of haematological parameters in HIV-infected and uninfected Rwandan women: a cross-sectional study  

PubMed Central

Objectives Although haematological abnormalities are common manifestations of HIV infection, few studies on haematological parameters in HIV-infected persons have been undertaken in sub-Saharan Africa. The authors assessed factors associated with haematological parameters in HIV-infected antiretroviral-naďve and HIV-uninfected Rwandan women. Study design Cross-sectional analysis of a longitudinal cohort. Setting Community-based women's associations. Participants 710 HIV-infected (HIV+) antiretroviral-naďve and 226 HIV-uninfected (HIV?) women from the Rwanda Women's Interassociation Study Assessment. Haematological parameters categorised as (abnormal vs normal) were compared by HIV status and among HIV+ women by CD4 count category using proportions. Multivariate logistic regression models using forward selection were fit. Results Prevalence of anaemia (haemoglobin (Hb) <12.0?g/dl) was higher in the HIV+ group (20.5% vs 6.3%; p<0.001), and increased with lower CD4 counts: ?350 (7.6%), 200–349 (16%) and <200 cells/mm3 (32.2%). Marked anaemia (Hb <10.0?g/dl) was found in 4.2% of HIV+ and none of the HIV? women (p<0.001), and was highest in HIV+ women with CD4 <200 cells/mm3 (8.4%). The HIV+ were more likely than HIV? women (4.2 vs 0.5%, respectively, p=0.002) to have moderate neutropenia with white blood cells <2.0×103 cells/mm3 and 8.4% of HIV+ women with CD4 <200 cells/mm3 had moderate neutropenia. In multivariate logistic regression analysis, BMI (OR 0.87/kg/m2, 95% CI 0.82 to 0.93; p<0.001), CD4 200–350 vs HIV? (OR 3.59, 95% CI 1.89 to 6.83; p<0.001) and CD4 <200 cells/mm3 vs HIV? (OR 8.09, 95% CI 4.37 to 14.97; <0.001) had large independent associations with anaemia. There were large independent associations of CD4 <200 cells/mm3 vs HIV? (OR 7.18, 95% CI 0.78 to 65.82; p=0.081) and co-trimoxazole and/or dapsone use (OR 5.69, 95% CI 0.63 to 51.45; p=0.122) with moderate neutropenia. Conclusions Anaemia was more common than neutropenia or thrombocytopenia in the HIV-infected Rwandan women. Future comparisons of haematological parameters in HIV-infected patients before and after antiretroviral therapy initiation are warranted.

Munyazesa, Elisaphane; Emile, Ivan; Mutimura, Eugene; Hoover, Donald R; Shi, Qiuhu; McGinn, Aileen P; Musiime, Stephenson; Muhairwe, Fred; Rutagengwa, Alfred; Dusingize, Jean Claude; Anastos, Kathryn

2012-01-01

143

Optimising a curriculum for clinical haematology and biochemistry in sports medicine: a Delphi approach  

PubMed Central

Objectives To investigate issues of curriculum in the context of a postgraduate sports medicine training programme, specifically in the field of clinical biochemistry and haematology. Methods Following the Delphi methodology, a series of sequential questionnaires was administered to curriculum developers, clinical teachers, examiners, and registrars. Results Agreement on a core syllabus for this subject with an indication of the core aims and objectives of teaching and learning in this field and the associated required skills and competencies. An indication of current and ideal teaching and learning methods and reasons for these preferences. A consensus of key features of a teaching module for this field and of appropriate methods of examination. Conclusions The data derived from this study, as well as the experience of engaging in it, will better inform curriculum developers, teachers, and students of one another's perceptions as to what is important in and appropriate to teaching and learning in this field of sports medicine. Engagement in the exercise and broader consideration of the outcomes by those who develop the curriculum, teach, and formulate the examination process will facilitate attainment of the ideal of well aligned teaching, learning, and examination in this specific field.

Fallon, K E; Trevitt, A C

2006-01-01

144

Serum galactomannan assay for the diagnosis of invasive aspergillosis in children with haematological malignancies.  

PubMed

Diagnostic efficacy of Galactomannan (GM) assay for invasive aspergillosis (IA) is variably reported. Data from developing countries are scant. Children with haematological malignancies and fever were enrolled prospectively. Blood sample for GM was drawn on the day of admission; levels were measured with Platellia Aspergillus enzyme immunoassay. Diagnostic criteria were adapted from EORTC-MSG-2002. Proven, probable and possible episodes were considered as the disease group. One hundred febrile episodes in 78 patients were evaluated. The mean age was 6.1?years. Majority (75%) episodes were in patients with acute lymphoblastic leukaemia. One episode each was diagnosed with proven and probable IA, while 23 were diagnosed with possible IA. Best results were obtained with a cut-off value of 1.0, with sensitivity, specificity, positive and negative predictive value of 60%, 93%, 75 and 87 respectively. The sensitivity dropped to 40%, at cut-off value of 1.5 and specificity was 38%, at a cut-off of 0.5. A higher value of GM correlated with pulmonary nodules (P?=?0.037) and mortality (P?=?0.001). GM assay is adjunctive to clinical/radiological evidence. A negative GM assay may not reassure the physician against the use of amphotericin in patients with febrile neutropenia, as it does not exclude the diagnosis of clinically relevant other fungal infections, particular mucormycosis. PMID:23369047

Jha, Ajaya K; Bansal, Deepak; Chakrabarti, Arunaloke; Shivaprakash, M R; Trehan, Amita; Marwaha, Ram K

2013-02-01

145

Lead poisoning: clinical, biochemical, and haematological aspects of a recent outbreak.  

PubMed Central

The clinical, biochemical, and haematological aspects of a recent outbreak of lead poisoning, in which exposure was related to the oxyacetylene cutting of red lead painted ironwork, were investigated. Initial suspicion was raised when a blood film showed punctate basophilia which remains a simple and useful method of picking up lead toxicity. Estimations of blood lead concentration and conventional laboratory data confirmed the diagnosis. Although there was prominent punctate basophilia, spectrophotometric analysis showed only negligible accumulation of pyrimidine-5'-nucleotides despite severe suppression of pyrimidine-5'-nucleotidase activity. The pattern of the red cell glycolytic intermediates, investigated for the first time, suggested that lead may also affect glycolysis at the hexokinase step. Once the diagnosis was made intravenous chelation treatment was begun with a rapid improvement in symptoms. Long term follow up is required to assess any sequelae of intoxication. These cases emphasise the classic features of lead poisoning, and despite the currently available diagnostic tests, lead intoxication may still go unrecognised unless a thorough occupational history is taken. Images

Pagliuca, A; Mufti, G J; Baldwin, D; Lestas, A N; Wallis, R M; Bellingham, A J

1990-01-01

146

Serum selenium concentration at diagnosis and outcome in patients with haematological malignancies.  

PubMed

We have previously reported presentation serum selenium level to be predictive of outcome in diffuse large B-cell lymphoma. This has now been studied in a further 430 patients, 163 with acute myeloid leukaemia (AML), 156 with Hodgkin Lymphoma (HL), and 111 with Follicular Lymphoma (FL). Serum selenium was below the UK normal reference range in 45% of patients, and correlated with serum albumin (r=0·24-0·46, P<0·001-0·003) in all tumour types. Independent predictors of presentation selenium were; French-American-British subtype and albumin (P<0·001 for both) in AML, haemoglobin (P=0·002) and B-symptoms (P=0·01) in HL, and albumin (P<0·001) in FL. In AML and HL, response to first line therapy was lower in patients with low serum selenium, but selenium was no longer predictive of response when other variables were entered into a multivariate model. Low selenium was also associated with a worse overall survival in FL [Hazard Ratio (HR) 2·3, 95% confidence interval (CI) 1·4, 4·0] and a trend to a worse overall survival in AML (HR 1·43, 95% CI 0·96, 2·13) by univariate Cox regression analysis, but not by multivariate analysis. In conclusion, low serum selenium is associated with a worse outcome in patients with haematological malignancies, but is not independently predictive, suggesting that it reflects other factors. PMID:21770918

Stevens, Jane; Waters, Rachel; Sieniawska, Christina; Kassam, Shireen; Montoto, Silvia; Fitzgibbon, Jude; Rohatiner, Ama; Lister, Andrew; Joel, Simon

2011-07-20

147

Effect of sample volume on yield of positive blood cultures from adult patients with haematological malignancy.  

PubMed Central

Six hundred and sixteen blood samples from patients with haematological malignancy were each distributed equally among three identical cells in a Malthus Microbiological Growth Analyser. The mean (SD) volumes inoculated into sets in which one, two, or three of the three bottles were positive were 37.7 (10.1) ml, 37.4 (12.9) ml, and 37.7 (10.5) ml, respectively. Overall, clinically important organisms were isolated from one bottle only with 18 cultures, from two bottles only with 19 cultures, and from all three bottles in a set with 104 cultures. If the yield from a single bottle inoculated with a mean volume of 12.6 ml blood is taken as 100%, the yield from 25.2 ml in two bottles was 110.7% and the yield from 37.7 ml in three bottles was 115.6%. The increased yield from increased volume was considerably lower than that reported from unselected groups of patients, which suggests that the magnitude of bacteriaemia is greater in patients with neutropenia. The isolation of infecting organisms from the blood of patients with neutropenia is, however, particularly important in directing chemotherapy and consequently 45 ml blood samples from these patients continue to be requested.

Brown, D F; Warren, R E

1990-01-01

148

Reversible phosphorylation in haematological malignancies: potential role for protein tyrosine phosphatases in treatment?  

PubMed

Most aspects of leukocyte physiology are under the control of reversible tyrosine phosphorylation. It is clear that excessive phosphorylation of signal transduction elements is a pivotal element of many different pathologies including haematological malignancies and accordingly, strategies that target such phosphorylation have clinically been proven highly successful for treatment of multiple types of leukemias and lymphomas. Cellular phosphorylation status is dependent on the resultant activity of kinases and phosphatases. The cell biology of the former is now well understood; for most cellular phosphoproteins we now know the kinases responsible for their phosphorylation and we understand the principles of their aberrant activity in disease. With respect to phosphatases, however, our knowledge is much patchier. Although the sequences of whole genomes allow us to identify phosphatases using in silico methodology, whereas transcription profiling allows us to understand how phosphatase expression is regulated during disease, most functional questions as to substrate specificity, dynamic regulation of phosphatase activity and potential for therapeutic intervention are still to a large degree open. Nevertheless, recent studies have allowed us to make meaningful statements on the role of tyrosine phosphatase activity in the three major signaling pathways that are commonly affected in leukemias, i.e. the Ras-Raf-ERK1/2, the Jak-STAT and the PI3K-PKB-mTOR pathways. Lessons learned from these pathways may well be applicable elsewhere in leukocyte biology as well. PMID:20659529

Ruela-de-Sousa, Roberta R; Queiroz, Karla C S; Peppelenbosch, Maikel P; Fuhler, Gwenny M

2010-07-24

149

Herpes simplex virus and intraoral ulcers in immunocompromised patients with haematologic malignancies.  

PubMed

Possible factors predisposing to the development of intraoral ulcers in immunocompromised patients with haematologic malignancies were investigated. Among 46 patients undergoing antineoplastic treatment, 18 developed an intraoral ulcer during the prospective study. Patients with or without ulcers were comparable with respect to underlying disease, presence of teeth, the qualitative composition of the aerobic and facultatively anaerobic oral microflora, herpes simplex virus (HSV) titer positivity, a past history of herpes labialis, leukocyte and thrombocyte counts, and duration of fever. Viral cultures from saliva revealed an association between the presence of HSV in saliva and the presence of intraoral ulcers. HSV was not isolated from the saliva of any patient without ulcers. Viral cultures from the ulcers revealed growth of HSV in 11 (61%) of the 18 patients with ulcers. HSV-positive ulcers were located more often on the alveolar process than elsewhere in the oral cavity. Ulcers on the lateral borders of the tongue and on the buccal mucosa were found only in dentate patients. Development of intraoral ulcers was not associated with an increase of the HSV titer in serum in any patient. It is concluded that some intraoral ulcers, especially when located on the alveolar process, are associated with and probably caused by HSV, while mechanical trauma during mastication seems likely to be involved in the pathogenesis of ulcers elsewhere in the oral cavity. PMID:2338087

Bergmann, O J; Mogensen, S C; Ellegaard, J

1990-03-01

150

Evaluation of the monocyte counting by two automated haematology analysers compared with flow cytometry.  

PubMed

The aim is to determine the monocyte count performance of the Bayer Diagnostics ADVIA120 and Coulter LH 750 automated haematology analysers and the results obtained by these two instruments were compared with those provided by Becton Dickinson FACScan flow cytometer using the combination of CD45/CD14 MoAb. Linearity and imprecision were also evaluated. The linearity of both instruments was good. Coulter LH 750 showed better precision (4.3%) than ADVIA 120 (9.0%) both within and between batch. A significant correlation (r = 0.973) was found between the LH 750 and the flow cytometry method, while a modest one was observed between the latter and the ADVIA 120 (r = 0.880). When comparing the percentage of monocytes by means of one-way anova and Tukey test, it was found that the LH 750 provided the closest results in comparison with flow cytometry, with no statistical difference between the means (mean difference MO% = 0.6); however the difference was statistically different between the ADVIA 120 and flow cytometry (mean difference MO% = -4.06). These data were confirmed by Altman-Bland and Deming regression analyses. PMID:15784123

Grimaldi, E; Carandente, P; Scopacasa, F; Romano, M F; Pellegrino, M; Bisogni, R; De Caterina, M

2005-04-01

151

Basic haematological values in antelopes--II. The Hippotraginae and the Tragelaphinae.  

PubMed

Basic haematological values in 49 animals of five species of the subfamily Hippotraginae, namely the roan antelope Hippotragus equinus, sable antelope Hippotragus niger, adax antelope Addax nasomaculatus, gemsbok oryx Oryx gazella gazella and scimitar horned oryx Oryx damah and in 51 individuals of five species in the subfamily Tragelaphinae, including the bushbuck Tragelaphus scriptus, nyala Tragelaphus angasi, greater kudu Tragelaphus strepsiceros, cape eland Tautotragus oryx and bongo Bocercus euryceros are reported. The erythrocyte counts were in the range of 12.62 X 10(12)/l in the gemsbok oryx to 6.44 X 10(12)/l in the bongo, the haematocrit values varied from 0.488 in the nyala to 0.380 in the roan antelope and for the haemoglobin highest levels (164.0 g/l) were noted in the adax antelope, and the lowest (105.5 g/l) in the bongo. Leukocyte counts were found mostly in the normal human range and varied from 7.17 X 10(9)/l in the adax antelope to 4.05 X 10(9)/l in the nyala, only in the greater kudu decreased values of 3.02 X 10(9) were estimated. These results are compared with findings taken from the literature, and with the normal human range. PMID:6149054

Pospísil, J; Kase, F; Vahala, J; Mouchová, I

1984-01-01

152

Detection of invasive pulmonary aspergillosis in haematological malignancy patients by using lateral-flow technology.  

PubMed

Invasive pulmonary aspergillosis (IPA) is a leading cause of morbidity and mortality in haematological malignancy patients and hematopoietic stem cell transplant recipients(1). Detection of IPA represents a formidable diagnostic challenge and, in the absence of a 'gold standard', relies on a combination of clinical data and microbiology and histopathology where feasible. Diagnosis of IPA must conform to the European Organization for Research and Treatment of Cancer and the National Institute of Allergy and Infectious Diseases Mycology Study Group (EORTC/MSG) consensus defining "proven", "probable", and "possible" invasive fungal diseases(2). Currently, no nucleic acid-based tests have been externally validated for IPA detection and so polymerase chain reaction (PCR) is not included in current EORTC/MSG diagnostic criteria. Identification of Aspergillus in histological sections is problematic because of similarities in hyphal morphologies with other invasive fungal pathogens(3), and proven identification requires isolation of the etiologic agent in pure culture. Culture-based approaches rely on the availability of biopsy samples, but these are not always accessible in sick patients, and do not always yield viable propagules for culture when obtained. An important feature in the pathogenesis of Aspergillus is angio-invasion, a trait that provides opportunities to track the fungus immunologically using tests that detect characteristic antigenic signatures molecules in serum and bronchoalveolar lavage (BAL) fluids. This has led to the development of the Platelia enzyme immunoassay (GM-EIA) that detects Aspergillus galactomannan and a 'pan-fungal' assay (Fungitell test) that detects the conserved fungal cell wall component (1 ?3)-?-D-glucan, but not in the mucorales that lack this component in their cell walls(1,4). Issues surrounding the accuracy of these tests(1,4-6) has led to the recent development of next-generation monoclonal antibody (MAb)-based assays that detect surrogate markers of infection(1,5). Thornton(5) recently described the generation of an Aspergillus-specific MAb (JF5) using hybridoma technology and its use to develop an immuno-chromatographic lateral-flow device (LFD) for the point-of-care (POC) diagnosis of IPA. A major advantage of the LFD is its ability to detect activity since MAb JF5 binds to an extracellular glycoprotein antigen that is secreted during active growth of the fungus only(5). This is an important consideration when using fluids such as lung BAL for diagnosing IPA since Aspergillus spores are a common component of inhaled air. The utility of the device in diagnosing IPA has been demonstrated using an animal model of infection, where the LFD displayed improved sensitivity and specificity compared to the Platelia GM and Fungitell (1 ? 3)-?-D-glucan assays(7). Here, we present a simple LFD procedure to detect Aspergillus antigen in human serum and BAL fluids. Its speed and accuracy provides a novel adjunct point-of-care test for diagnosis of IPA in haematological malignancy patients. PMID:22473419

Thornton, Christopher; Johnson, Gemma; Agrawal, Samir

2012-03-22

153

A data management software for the Sysmex NE 8000 haematology analyser.  

PubMed

The Sysmex NE 8000 (TOA-Japan) is a haematology analyser that performs blood cells count and leukocyte differential count. For facilitating the work of technical validation, we developed a software adapted to any IBM or compatible PC running under MS-DOS, to manage the analyser. Data are automatically collected via the RS-232 interface from the analyser or keyed in for the other techniques. The software deals with 64 different analyses entirely "user defined". Six technical alarms of the analyser are taken in account for red or white cells and platelets. An "electronic worksheet" presents the results or alarms with 10 patients to a page. This enables the lab technician to assess the coherence of the various data and to perform verifications or complementary tests if necessary. As an option, a blinking asterisk can signal any results out of predetermined range. By moving the cursor through the table, a test result can be deleted, modified or added. A function displays the patient previous files in a window because the data are recorded in long term archives at the end of the day. This long term recording allows a search of previous files to decide additional tests if the patient is unknown. If the patient is known, with additional tests previously performed, this procedure is time saving. A daily archive function classifies and prints the whole day's work in alphabetical order. A protocol of communication allows a connection to a mainframe computer Bayer-Technicon. This program and the user's manual are free of charge, available on request from J. P. Cambus. PMID:8814394

Cambus, J P; Nguyen, F; Cambus, F

1996-07-01

154

Some changes in the haematological parameters of rainbow trout ( Oncorhynchus mykiss ) exposed to three artificial photoperiod regimes  

Microsoft Academic Search

Haematological parameters are often used as health status and stress indicators in fish. However, information on the effects\\u000a of artificial photoperiods on these parameters is scarce and ambiguous. The consequences of three different artificial photoperiod\\u000a regimes [light\\/day 12 h:12 h (LD 12:12) for 150 days as controls; LD 14:10 and LD 24:0, both for an initial 60 days, and then\\u000a LD 12:12 for the remaining 90 days] were evaluated

A. E. Valenzuela; V. M. Silva; A. E. Klempau

2007-01-01

155

Evolution of clinical, haematological and biochemical findings in young dogs naturally infected by vector-borne pathogens.  

PubMed

Longitudinal studies evaluating the evolution of clinical, haematological, biochemical findings in young dogs exposed for the first time to multiple vector-borne pathogens have not been reported. With the objective of assessing the evolution of clinical, haematological and biochemical findings, these parameters were serially monitored in naturally infected dogs throughout a 1-year follow-up period. Young dogs, infected by vector-borne pathogens based on cytology or polymerase chain reaction, were examined clinically and blood samples were obtained at seven different follow-up time points. Dogs were randomized to group A (17 dogs treated with a spot-on formulation of imidacloprid 10% and permethrin 50%) or to group B (17 dogs untreated). In addition, 10 4-month-old beagles were enrolled in each group and used as sentinel dogs. At baseline, Anaplasma platys was the most frequently detected pathogen, followed by Babesia vogeli, Bartonella spp., Ehrlichia canis and Hepatozoon canis. Co-infections with A. platys and B. vogeli, followed by E. canis and B. vogeli, A. platys and H. canis and A. platys and Bartonella spp. were also diagnosed. In dogs from group B, abnormal clinical signs were recorded at different time points throughout the study. No abnormal clinical signs were recorded in group A dogs. Thrombocytopenia was the most frequent haematological alteration recorded in A. platys-infected dogs, B. vogeli-infected dogs and in dogs co-infected with A. platys and B. vogeli or A. platys and Bartonella spp. Lymphocytosis was frequently detected among dogs infected with B. vogeli or co-infected with A. platys and B. vogeli. Beagles were often infected with a single pathogen rather than with multiple canine vector-borne pathogens. There was a significant association (p<0.01) between tick infestation and A. platys or B. vogeli, as single infections, and A. platys and B. vogeli or A. platys and Bartonella spp. co-infections. This study emphasizes the clinical difficulties associated with assigning a specific clinical sign or haematological abnormality to a particular canine vector-borne disease. PMID:21106311

de Caprariis, Donato; Dantas-Torres, Filipe; Capelli, Gioia; Mencke, Norbert; Stanneck, Dorothee; Breitschwerdt, Edward B; Otranto, Domenico

2010-10-16

156

Could interleukin-15 potentiate histone deacetylase inhibitor effects in haematological malignancy?  

PubMed

Despite significant progress in cancer therapy, prognosis in acute leukaemia remains dismal, and the development of new therapies is urgently warranted: in acute myeloid leukaemia, the current cure rate is of 30-40% in younger and much less in older patients. Chromatin remodeling through histone acetylation is one of the major mechanisms of transcriptional control of genes, and is involved in 'gene silencing' of antioncogenes in various tumour cells. Chromatin remodeling is also involved in transcriptional control of other genes, such as NKG2D ligand genes. Histone deacetylases and acetyltransferases are involved in the epigenetic regulation of gene expression, and increased/decreased activity of histone deacetylases has been reported in several cancer types. Histone deacetylase inhibitors were reportedly active in many cancers including hematological malignancies, and have been shown in numerous experiments to reduce cancer cell growth and enhance cell differentiation, growth arrest and apoptosis. In acute myeloid leukaemia, histone deacetylase inhibitors alone had limited efficacy, but their combination with other anticancer agents yielded promising results. Interleukin (IL)-15 is regarded with great hope in the immunotherapy of cancer, and IL-15-activated cytokine-induced killer cells showed potent antileukemic activity both in vitro and in vivo. IL-15 increases expression of NKG2D and its ligands and can increase natural killer cell mediated cytotoxicity against tumour cells. The administration of IL-15 was recently shown to be safe in preclinical models, and there are ongoing clinical trials of IL-15 in patients with cancer and HIV infection. We hypothesise that IL-15 will synergise with histone deacetylase inhibitors in increasing the levels of activatory NKG2D receptors on natural killer and CD8(+) T cells and of their ligands, the MHC class I related molecule A and B, on tumor cells, and will enhance innate immune antitumour responses in acute myeloid leukaemia and other haematological malignancies. Up-regulation of NKG2D-NKG2D-ligand antitumour immune response by combining histone deacetylase inhibitors with IL-15 has the potential to improve the efficacy of acute myeloid leukaemia treatment. PMID:23669372

Zdrenghea, Mihnea T

2013-05-10

157

Biochemical and haematological evaluation of repeated dose exposure of male Wistar rats to an ethanolic extract of Artemisia annua.  

PubMed

Artemisia annua is widely used for the treatment of malaria and other disorders. In a previous study, the artemisinin concentration in the dry leaves of A. annua grown under humid tropical conditions was determined to be 1.098% using reversed phase high performance liquid chromatography. In the current study, biochemical and haematological evaluations of ethanolic leaf extracts derived from such plants (EAA) were carried out in 20 male Wistar rats. Rats were divided into four study groups of saline-treated (control) and test groups exposed orally to graded doses of EAA for 28 days. The results showed that the liver function and haematological indices, and testosterone levels were not adversely affected. High density lipoprotein -cholesterol was reduced at 100?mg/kg of EAA, atherogenic index as well as low density lipoprotein -cholesterol was raised, and glucose concentration was reduced significantly at the 100 and 200?mg/kg of EAA (p?

Eteng, Mbeh U; Abolaji, Amos O; Ebong, Patrick E; Brisibe, Ebiamadon Andi; Dar, Ahsana; Kabir, Nurul; Iqbal Choudhary, M

2012-06-27

158

Development and application of biochemical and haematological reference intervals to identify unhealthy green sea turtles (Chelonia mydas).  

PubMed

Biochemical and haematological reference intervals (RIs) have been reported for sea turtles, but their value for ante-mortem disease diagnosis may be limited due to small sample sizes and outdated statistical analyses. In the present study, 290 green sea turtles (Chelonia mydas) were captured, clinically assessed and blood sampled. Of these, 211 were classified as 'clinically healthy' and 25 as 'clinically unhealthy'. RIs were estimated using data from the healthy turtles and compared with blood values from the unhealthy animals. All of the unhealthy animals had plasma biochemical and haematological values outside one or more RIs (albumin, 48% of unhealthy animals; alkaline phosphatase, 35%; aspartate transaminase, 13%; creatinine, 30%; globulin, 3%; glucose, 34%; lactic dehydrogenase, 26%; phosphorus, 22%; sodium, 13%; thrombocytes, 57%; and monocytes, 5%). Among small immature turtles, those with Chelonibia testudinaria plastron barnacle counts 20 were three times more likely to be unhealthy than those with no barnacles. In addition, small immature and mature turtles were more likely to be unhealthy than large immature turtles. PMID:19709912

Flint, Mark; Morton, John M; Limpus, Colin J; Patterson-Kane, Janet C; Murray, Peter J; Mills, Paul C

2009-08-25

159

Comparison of the performance, carcass characteristics and haematological parameters of broiler chicks reared in cage and floor.  

PubMed

This study was conducted to determine the effect of housing systems on the performance, carcass characteristics and the haematology of 150 four-week old Anak titan strains of broiler chicks in a completely randomized design. The birds were allocated randomly into two treatment (cage and floor) groups of three replications containing 25 birds per replicate. They were maintained for a period of 4 weeks and data were collected on; final body weight, weight gain, feed intake, feed:gain and mortality. At the 56th week, carcass yield, cut-up parts and organs weights as well as some parameters were measured. The results showed that feed intake and feed:gain were significantly (p < 0.05) higher in the cage system. Dressing percentage and breast weight were higher (p < 0.05) in the floor housing system while drumstick and lung weight were higher in the cage system. No significant (p > 0.05) difference was obtained in the haematology. It was then concluded that an improvement in the floor system could result into a better performance and an increased carcass yield. PMID:18817178

Sogunle, O M; Egbeyale, L T; Bajomo, T T; Bamigboje, O V; Fanimo, A O

2008-02-01

160

Protective effects of vitamin C against haematological and biochemical toxicity induced by deltamethrin in male Wistar rats.  

PubMed

Deltamethrin is a synthetic pyrethroid insecticide. It is known for its wide toxic manifestations. The present experiment pertains to the protective role of vitamin C against haematological and biochemical toxicity induced by deltamethrin during 4 weeks. Male Wistar rats were divided into four groups of eight each: Group I served as control rats; Group II received deltamethrin (1.28 mg/kg BW) in drinking water. Group III received both deltamethrin and vitamin C (200mg/kg BW; by i.p. injection); Group IV received vitamin C (200mg/kg BW). Exposure of rats to deltamethrin caused significant changes of some haematological parameters (red blood cells (RBC), haemoglobin (Hb), haematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet (Plt) and white blood cells (WBC)) in treated rats compared to controls. Significant increases in the levels of hepatic markers enzymes (alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), ?-Glutamyl transpeptidase (?-GT)). Furthermore, renal markers such as urea and creatinine were increased in deltamethrin treated rats. Additionally, serum cholesterol and lipid peroxidation were significantly enhanced. Co-administration of vitamin C to the group III restored all the parameters cited above to near-normal values. Therefore, our investigation revealed that vitamin C appeared to be a promising agent for protection against deltamethrin-induced toxicity. PMID:21514672

Mongi, Saoudi; Mahfoud, Messarah; Amel, Boumendjel; Kamel, Jamoussi; Abdelfattah, El Feki

2011-04-22

161

Genetic analysis of haematological and plasma biochemical parameters in the Spanish purebred horse exercised on a treadmill.  

PubMed

The novel aim of this study was to describe the reference values of different haematological and biochemical parameters in the Spanish purebred horse (Andalusian, SPB) in each of the stages of a programmed exercise on a treadmill system, and to establish heritability and genetic correlations for these haematological and biochemical parameters. For this, 94 young SPB male horses (4.22 ± 2.27 years old) were used. An increasing intensity exercise test at 4, 5, 6 and 7 m/s was carried out on a treadmill (6% inclination). Total red blood cells, total white blood cells, neutrophils and lymphocytes counts; haematocrit, haemoglobin, lactate, uric acid, creatinine and total plasma proteins concentrations and aspartate transaminase, lactate dehydrogenase, creatine-quinase activities were determined. To conclude: (i) the reference values for each parameter were determined for each of the exercise test stages (ii) all the parameters analysed manifested a medium-high heritability and a high repeatability. These results will, in the near future, determine the measuring guidelines for improving the SPB horse's athletic ability on an objective treadmill system and for selecting these animals in response to those parameters. PMID:23702348

Escribano, B M; Molina, A; Valera, M; Tovar, P; Agüera, E I; Santisteban, R; Vivo, R; Agüera, S; Rubio, M D

2013-05-24

162

Haematological profile of crossbred dairy cattle to monitor herd health status at medium elevation in Central Himalayas.  

PubMed

Haematological profile-haemoglobin concentration (Hb), total erythrocytes count (TEC), packed cell volume (PCV), erythrocyte indices-mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were studied in crossbred dairy cattle (Holstein Friesian x Sahiwal) under various physiological states: non-pregnant heifers (NPH), pregnant heifers (PH), empty dry cows (EDC), pregnant lactating cows (PLC), medium yield early lactating cows (MYELC) and high yield early lactating cows (HYELC) during summer and winter seasons at 1700 metres altitude from mean sea level in the Central Himalayas. On comparison of annual means, the highest values of Hb and PCV were recorded in PH and of TEC in NPH, whereas the lowest values of these parameters were found in EDC. The Hb and TEC tended to decrease with increasing milk yield. Comparison of annual means of erythrocyte indices revealed the highest MCV and MCH in EDC, which simultaneously showed the lowest MCHC. Significant seasonal variations in haematological profile were recorded. The overall group mean (OGM) of Hb, MCV, MCH and MCHC was found to be significantly higher (P < 0.01) during summer whereas the TEC and PCV showed higher OGM (P < 0.01) during the winter season. PMID:11020365

Kumar, B; Pachauri, S P

2000-10-01

163

Investigation on Plasmodium falciparum and Plasmodium vivax infection influencing host haematological factors in tribal dominant and malaria endemic population of Jharkhand.  

PubMed

The study was undertaken to elucidate the association of host haematological and biochemical indices in Plasmodium falciparum and Plasmodium vivax malaria in order to explore whether these parameters are unique to disease or act as a potential diagnostic marker. Haematological and biochemical parameters in 106 malarial patients and 33 healthy subjects were evaluated. Following parameters were significantly lower in all infection types (P. vivax, P. falciparum and mixed infection); haemoglobin, blood sugar, PCV and blood urea, while ESR is significantly higher in all types of infection whereas serum bilirubin and creatinine are significantly higher except mixed and vivax infection, respectively. Interestingly, parasitaemia, temperature and age are significantly correlated with blood urea, blood sugar and ESR respectively in vivax infection whereas parasitaemia with PCV and blood sugar and age with PCV in falciparum infection. Malaria infected subjects exhibited alterations in some haematological parameters with low haemoglobin, blood sugar and PCV whereas elevated ESR and serum bilirubin being the important findings observed in our study. These evaluations could be considered to be reliable clinical and biochemical markers for promising diagnostic potential during clinical malarial infection in combination with other genetic and classical microscopic parameters. Haematological evaluation could help in prompt and accurate diagnosis and prevent disease progression by facilitating physicians in clinical correlation for better drug regime. PMID:23961236

Hussain, Mohammad Mobassir; Sohail, Mohammad; Abhishek, Kumar; Raziuddin, Mohammad

2013-02-01

164

Effect of Ramadan fasting on some biochemical and haematological parameters in Tunisian youth soccer players undertaking their usual training and competition schedule  

Microsoft Academic Search

Competitive Muslim athletes often have to train and compete during the holy month of Ramadan when they abstain from food and drink from sunrise to sunset. In this study, we investigated the effect of Ramadan fasting on some biochemical and haematological parameters in 78 Tunisian junior male soccer players aged 16–19 years who continued their usual schedule of daily training

Ronald J. Maughan; John B. Leiper; Zakia Bartagi; Rym Zrifi; Yacine Zerguini; Jiri Dvorak

2008-01-01

165

Effects of prolonged strenuous exercise (marathon running) on biochemical and haematological markers used in the investigation of patients in the emergency department  

Microsoft Academic Search

Objectives: To investigate the effects of strenuous exercise on commonly used biochemical and haematological variables in subjects running the 2002 London marathon.Methods: 34 healthy volunteers (7 female, 27 male) were recruited for the study. Blood was taken before the start (at registration) and immediately after completion of the marathon. Samples were analysed for urea and electrolytes, liver function tests, creatine

J E Smith; G Garbutt; P Lopes; D Tunstall Pedoe

2004-01-01

166

Evaluation of cinnamon and garlic as antibiotic growth promoter substitutions on performance, immune responses, serum biochemical and haematological parameters in broiler chicks  

Microsoft Academic Search

This study was performed to investigate the effect of dietary supplementation with cinnamon and garlic powder as growth promoter agents on performance, carcase traits, immune responses, serum biochemistry, haematological parameters and thigh meat sensory evaluation in broilers. A total of 288, day-old male broilers (Ross 308) were randomly assigned to six treatments with four replicates. The dietary treatments consisted of

Mehdi Toghyani; Majid Toghyani; Abbasali Gheisari; Gholamreza Ghalamkari; Shahin Eghbalsaied

2011-01-01

167

Responses to submaximal treadmill exercise and training in the horse: changes in haematology, arterial blood gas and acid base measurements, plasma biochemical values and heart rate  

Microsoft Academic Search

Four standardbred horses with subcutaneously relocated carotid arteries were given a seven week training programme of treadmill exercise at a gradient of 19 per cent in order to assess if there were any effects of exercise and training on haematology, arterial blood gas and acid base measurements, plasma biochemistry and heart rate. The exercise consisted of one minute walking at

RJ Rose; JR Allen; JH Stewart; W Chan

1983-01-01

168

Haematological and serum biochemical parameters of West African Dwarf goats fed dried cassava leaves-based concentrate diets.  

PubMed

The study was conducted to investigate the effects of feeding different levels of dried cassava leaves at 0%, 20%, 40% and 60%, respectively, using guinea grass as basal feed, on the haematological and serum biochemical parameters of West African Dwarf (WAD) goats. The study lasted for 116 days during which haematological and serum biochemical parameters were monitored in 40 male goats before and after, using a completely randomized design. At the start of the experiment, packed cell volume (PCV) ranged from 21.5% to 25.5% while haemoglobin concentration (Hb) and RBC significantly (P??0.05). Lymphocyte reduced significantly (P??0.05) at the 0% to 40% levels and reduced at the 60% level of dried cassava leaves inclusion. At the start of the experiment, values for glucose significantly (P?haematological and serum biochemical parameters of WAD goats and could therefore be included in ruminant diets up to 60%. PMID:21744028

Oni, Adebayo Olusoji; Arigbede, Oluwasanmi Moses; Sowande, Olusiji Sunday; Anele, Uchenna Young; Oni, Oluwakemi Oluremilekun; Onwuka, Chryss Friday Ijeoma; Onifade, Olufemi Sunday; Yusuf, Kafayat Omowumi; Dele, Peter Aniwe; Aderinboye, Ronke Yemisi

2011-07-09

169

Repeated transfusions of autologous cytokine-induced killer cells for treatment of haematological malignancies in elderly patients: a pilot clinical trial.  

PubMed

The elderly population is susceptible to haematological malignancies, and these elderly patients are intolerant to cytotoxic drugs. Therefore, the exploration of a safe and reliable strategy exclusive of chemotherapy is critical in improving the prognosis of elderly patients with haematological malignancies. We evaluated the safety and the efficacy of autologous cytokine-induced killer (CIK) cells combined with recombinant human interleukin 2 (rhIL-2) in the treatment of haematological malignancies in elderly patients. Peripheral blood mononuclear cells were isolated from 20 elderly patients with haematological malignancies, then augmented by priming with interferon gamma, rhIL-2 and CD3 monoclonal antibody. The autologous CIK cells (2-3 × 10(9)) were transfused back to patients, followed by a subcutaneous injection of IL-2 (1 mU/day) for 10 consecutive days. The regimen was repeated every 4 weeks. The host cellular immune function, tumour-related biological parameters, imaging characteristics, disease condition, quality of life and survival time were assessed. Fourteen patients received 8 cycles of transfusion and 6 received 4 cycles. No adverse effects were observed. The percentages of CD3(+), CD3(+) CD8(+) and CD3(+) CD56(+) cells were significantly increased (p < 0.05), and the levels of serum ?2 microglobulin and lactate dehydrogenase (LDH) were markedly decreased (p < 0.05) after autologous CIK cell transfusion. Cancer-related symptoms were profoundly alleviated, as demonstrated by the improved quality of life (p < 0.01). Complete remission was observed in 11 patients, persistent partial remission in 7 patients and stable disease in 2 patients. At the end of follow-up, the mean survival time was 20 months. Transfusion with autologous CIK cells plus rhIL-2 treatment is safe and effective for treating haematological malignancies in elderly patients. PMID:22972689

Yang, Bo; Lu, Xue-chun; Yu, Rui-li; Chi, Xiao-hua; Liu, Yang; Wang, Yao; Dai, Han-ren; Zhu, Hong-li; Cai, Li-li; Han, Wei-dong

2011-08-23

170

Clinical and haematological evaluation of beta thalassaemia intermedia characterised by unusually low Hb F and increased Hb A2: beta thalassaemia intermedia II.  

PubMed Central

A total of 15 patients from different families with thalassaemia intermedia was studied. Haematological studies showed that the fetal haemoglobin was only slightly raised, being between 2 and 11.5% of the total haemoglobin. Haemoglobin A2 was high in all cases. The family study indicated that homozygosity or compound heterozygosity for beta thalassaemia was present in five patients, while dominant inheritance was observed in three. In seven patients family studies were not sufficient to predict the genotype. Haematological findings in the parents of the homozygous patients were as severe as those seen in common Hb A2 beta thalassaemia traits. The decrease in MCH and MCV was more severe and the Hb A2 higher in homozygous patients than in cases of common beta thalassaemia major (p less than 0.01, p less than 0.01, and p less than 0.001 respectively). The imbalance in in vitro globin synthesis was more severe in classical beta thalassaemia major than in homozygous patients in this study (p less than 0.01). However, the imbalance in alpha/non-alpha synthetic ratios showed variation among the homozygous and heterozygous patients in this study (2.1 to 4.0). Haematological severity and Hb F value showed some slight variation among affected persons of the same family in the case of patients with severe beta thalassaemia heterozygosity. The G gamma/A gamma ratio of haemoglobin F was found to be close to that of the adult level. Haematological studies suggested that clinical and haematological findings were more severe in patients with homozygous beta thalassaemia than in patients with heterozygosity for beta thalassaemia. The prevalence of thalassaemia intermedia with low Hb F and increased Hb A2 was found to account for 3% of the Turkish beta thalassaemic patients diagnosed before the age of 8 years. Images

Gurgey, A; Kayin, S; Kansu, E; Altay, C

1985-01-01

171

Parameters of haematology, clinical chemistry and lipid metabolism in the common marmoset and alterations under stress conditions.  

PubMed

Common marmosets are suitable non-human primate models for many human diseases. Standard values for blood parameters are required to evaluate physiological and pathological situations. Two studies were conducted: study I to determine standard values and study II to examine these under changed housing conditions. In study I, all parameters for clinical chemistry were similar in range for both genders with these specifics: male marmosets had significantly higher total and LDL cholesterol levels than females, whereas the mean corpuscular volume and the mean corpuscular haemoglobin were significantly lower than in females. In study II, glucose, lymphocytes and salivary cortisol were significantly lower, and faecal cortisol was increased during the change of housing conditions. In conclusion, standard values for haematology and clinical chemistry for the common marmoset were determined. Further on, parameters that are influenced by relocation stress and its importance for experimental results are described. PMID:22765494

Kuehnel, F; Grohmann, J; Buchwald, U; Koeller, G; Teupser, D; Einspanier, A

2012-07-06

172

The concentrations of methaemoglogin, carboxyhaemoglobin and some haematological parameters in tobacco snuff addicts in Igbo of Nigeria.  

PubMed

Methaemoglobin, carboxyhaemoglobin concentrations and some haematological parameters were studied in fifty tobacco snuff addicts (40 males and 10 females) in some villages of Anambra State, Nigeria. The aim was to investigate possible adverse effects of tobacco snuff in addicts in Igbos of Anambra State. Fifty apparently healthy persons (25 males and 25 females) who do not inhale snuff were used as controls. The age range of tests and control subjects was 25-65 years. The results showed no statistically significant difference when the tests group was compared with the control group. A comparison of the results on the basis of sex, age and period of exposure, showed no significant differences. Blood picture in test and control groups was normocytic and normochromic. The results suggest that tobacco snuff may not have any adverse effect on haemoglobin metabolism and erythropoiesis. PMID:18379614

Ureme, S O; Ibeagha, I D; Maduka, I G; Ibeagbulam, O G

173

Allogeneic and autologous transplantation for haematological diseases, solid tumours and immune disorders: definitions and current practice in Europe.  

PubMed

The Accreditation Subcommittee of the EBMT regularly publishes special reports on current practice of haemopoietic stem cell transplantation for haematological diseases, solid tumours and immune disorders in Europe. Major changes have occurred since the first report was published in 1996. Haemopoietic stem cell transplantation today includes grafting with allogeneic and autologous stem cells derived from bone marrow, peripheral blood and cord blood. With reduced intensity conditioning regimens in allogeneic transplantation, the age limit has increased, permitting the inclusion of older patients. New indications have emerged such as autoimmune disorders and AL amyloidosis for autologous, and solid tumours for allogeneic transplants. The introduction of alternative therapies has challenged well-established indications such as imatinib for chronic myeloid leukaemia. An updated report with revised tables and operating definitions is presented here. PMID:16444286

Ljungman, P; Urbano-Ispizua, A; Cavazzana-Calvo, M; Demirer, T; Dini, G; Einsele, H; Gratwohl, A; Madrigal, A; Niederwieser, D; Passweg, J; Rocha, V; Saccardi, R; Schouten, H; Schmitz, N; Socie, G; Sureda, A; Apperley, J

2006-03-01

174

Allogeneic and autologous transplantation for haematological diseases, solid tumours and immune disorders: current practice in Europe 2009.  

PubMed

The European Group for Blood and Marrow Transplantation regularly publishes special reports on the current practice of haematopoietic SCT for haematological diseases, solid tumours and immune disorders in Europe. Major changes have occurred since the first report was published. HSCT today includes grafting with allogeneic and autologous stem cells derived from BM, peripheral blood and cord blood. With reduced-intensity conditioning regimens in allogeneic transplantation, the age limit has increased, permitting the inclusion of older patients. New indications have emerged, such as autoimmune disorders and AL amyloidosis for autologous HSCT and solid tumours, myeloproliferative syndromes and specific subgroups of lymphomas for allogeneic transplants. The introduction of alternative therapies, such as imatinib for CML, has challenged well-established indications. An updated report with revised tables and operating definitions is presented. PMID:19584824

Ljungman, P; Bregni, M; Brune, M; Cornelissen, J; de Witte, T; Dini, G; Einsele, H; Gaspar, H B; Gratwohl, A; Passweg, J; Peters, C; Rocha, V; Saccardi, R; Schouten, H; Sureda, A; Tichelli, A; Velardi, A; Niederwieser, D

2009-07-06

175

Allogeneic and autologous transplantation for haematological diseases, solid tumours and immune disorders: definitions and current practice in Europe.  

PubMed

The Accreditation Sub-Committee of the EBMT regularly publishes special reports on current practice of haemopoietic stem cell transplantation for haematological diseases, solid tumours and immune disorders. Major changes have occurred since the last report in 1998. Haemopoietic stem cell transplantation today includes allogeneic and autologous stem cells derived from bone marrow, peripheral blood and cord blood. With reduced intensity conditioning regimens in allogeneic transplantation, the age limit has increased, permitting the inclusion of older patients. New indications have emerged, such as autoimmune disorders and AL amyloidosis for autologous, and solid tumours for allogeneic transplants. Other indications, such as autologous transplantation for breast cancer have been challenged. An updated report with revised tables and operating definitions is presented here. PMID:12180107

Urbano-Ispizua, A; Schmitz, N; de Witte, T; Frassoni, F; Rosti, G; Schrezenmeier, H; Gluckman, E; Friedrich, W; Cordonnier, C; Socie, G; Tyndall, A; Niethammer, D; Ljungman, P; Gratwohl, A; Apperley, J; Niederwieser, D; Bacigalupo, A

2002-04-01

176

Chlorpyrifos-induced changes in oxidant/antioxidant status and haematological parameters of Cyprinus carpio carpio: ameliorative effect of lycopene.  

PubMed

The present study was carried out to investigate the potential ameliorative effects of lycopene against chlorpyrifos (CPF) toxicity in carp. The fish were divided into 7 different experimental groups and received the following treatments: Group 1, control; Group 2, orally administered corn oil; Group 3, oral lycopene (10 mg kg(-1) body weight); Group 4, exposure to 0.040 mg L(-1) CPF; Group 5, exposure to 0.040 mg L(-1) CPF plus oral administration of 10 mg kg(-1) lycopene; Group 6, exposure to 0.080 mg L(-1) CPF; and Group 7, exposure to 0.040 mg L(-1) CPF plus oral administration of 10 mg kg(-1) lycopene. Treatment was continued for 14 d and samples of the blood and tissue (liver, kidney, and gill) were collected at the end of the experiment and analysed for their oxidant-antioxidant status, including the malondialdehyde (MDA) levels, and the superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activity. The samples were also measured for changes in the haematological parameters, such as the red blood cell (RBC) and white blood cell (WBC) counts, the haemoglobin concentration (Hb), the haematocrit (Ht) level, and the erythrocyte indices: the mean corpuscular volume (MCV), the mean corpuscular haemoglobin (MCH) and the mean corpuscular haemoglobin concentration (MCHC). The findings of this study demonstrated that CPF had a negative effect on the haematological parameters and the antioxidant enzyme activities of the fish; this toxic effect was neutralised by the administration of lycopene. The present results suggest that lycopene (10 mg kg(-1)) can be effective in the protection of CPF-induced toxicity in fish. PMID:23312461

Ural, Mevlüt ?ener

2013-01-09

177

The protective effect of hydrated sodium calcium aluminosilicate against haematological, biochemical and pathological changes induced by Zearalenone in mice.  

PubMed

Hydrated sodium calcium aluminosilicate (HSCAS), an anticaking agent for mixed feed, was added alone or simultaneously with a toxic Zearalenone (ZEN) dose to balb/c mice and was evaluated for its ability to restore damages induced by ZEN. The latter is a mycotoxin produced by fusarium genera; it is mainly known to induce several toxic effects such as hepatotoxicity, immunotoxicity and nephrotoxicity on animals and humans. The experimental approach consisted of eight treatments of six mice each by 400 mg/kg bw or 5 g/kg bw of HSCAS. Two experimental groups have received respectively ZEN alone at 40 (8% of LD50) and at 500 mg/kg bw (LD50). Two other groups have received ZEN at 40 or 500 mg/kg bw combined respectively with HSCAS at 400 mg/kg bw and 5 g/kg bw. The control groups received water or olive oil. Forty-eight hours after treatment, blood samples were collected for haematological and serum biochemical parameters measurements. ZEN treatment significantly increased hematocrit, haemoglobin, white blood cells: lymphocytes, eosinophils, neutrophils, monocytes and the most of biochemical serum parameters; it significantly reduced platelets and induced degenerative changes in the hepatic and renal tissues; while, the mixture of HSCAS with ZEN induced a reestablishment of haematological parameters, levels of serum biochemical enzyme activities and histological pictures of both liver and kidney. It also prevented general toxicity of ZEN. This was observed by the shift of LD50 for this toxin. Thus, our data strongly suggested that deleterious effects of ZEN could be overcome or, at least, significantly were diminished by HSCAS. Moreover, this sorbent by itself did not show any toxic effects. PMID:16563452

Abbčs, Samir; Ouanes, Zouhour; ben Salah-Abbčs, Jalila; Houas, Zohra; Oueslati, Ridha; Bacha, Hassen; Othman, Omar

2006-03-24

178

Visualising the Cross-Level Relationships between Pathological and Physiological Processes and Gene Expression: Analyses of Haematological Diseases  

PubMed Central

The understanding of pathological processes is based on the comparison between physiological and pathological conditions, and transcriptomic analysis has been extensively applied to various diseases for this purpose. However, the way in which the transcriptomic data of pathological cells relate to the transcriptomes of normal cellular counterparts has not been fully explored, and may provide new and unbiased insights into the mechanisms of these diseases. To achieve this, it is necessary to develop a method to simultaneously analyse components across different levels, namely genes, normal cells, and diseases. Here we propose a multidimensional method that visualises the cross-level relationships between these components at three different levels based on transcriptomic data of physiological and pathological processes, by adapting Canonical Correspondence Analysis, which was developed in ecology and sociology, to microarray data (CCA on Microarray data, CCAM). Using CCAM, we have analysed transcriptomes of haematological disorders and those of normal haematopoietic cell differentiation. First, by analysing leukaemia data, CCAM successfully visualised known relationships between leukaemia subtypes and cellular differentiation, and their characteristic genes, which confirmed the relevance of CCAM. Next, by analysing transcriptomes of myelodysplastic syndromes (MDS), we have shown that CCAM was effective in both generating and testing hypotheses. CCAM showed that among MDS patients, high-risk patients had transcriptomes that were more similar to those of both haematopoietic stem cells (HSC) and megakaryocyte-erythroid progenitors (MEP) than low-risk patients, and provided a prognostic model. Collectively, CCAM reveals hidden relationships between pathological and physiological processes and gene expression, providing meaningful clinical insights into haematological diseases, and these could not be revealed by other univariate and multivariate methods. Furthermore, CCAM was effective in identifying candidate genes that are correlated with cellular phenotypes of interest. We expect that CCAM will benefit a wide range of medical fields.

Ono, Masahiro; Tanaka, Reiko J.; Kano, Manabu; Sugiman, Toshio

2013-01-01

179

Selected Haematological and Biochemical Parameters of Blood in Rats After Subchronic Administration of Vanadium and\\/or Magnesium in Drinking Water  

Microsoft Academic Search

The purpose of these studies was to evaluate the effect of selected vanadium and magnesium doses on certain haematological\\u000a and biochemical blood parameters in rats. Outbred 2-month-old, albino male Wistar rats received for a period of 6 weeks, as\\u000a a sole drinking liquid, the following water solutions: group II, sodium metavanadate (SMV) at a concentration of 0.125 mg\\u000a V\\/mL; group

Agnieszka ?cibior; Halina Zaporowska; Jaros?aw Ostrowski

2006-01-01

180

The effects of clofibrate on plasma glucose, lipoproteins, fibrinogen, and other biochemical and haematological variables in patients with mature onset diabetes mellitus  

Microsoft Academic Search

We have studied the effects of clofibrate treatment on glucose tolerance and plasma insulin, plasma triglyceride, cholesterol and non-esterified fatty acid (NEFA) levels, and on various haematological variables (including plasma fibrinogen level, red cell flexibility, whole blood viscosity, and plasma ß-thromboglobulin level) in patients with mature-onset diabetes. Twenty-two patients (11 men and 11 women) were randomly allotted to treatment with

G. D. Calvert; L. Blight; J. Franklin; J. Oliver; P. Wise; A. S. Gallus

1980-01-01

181

Application of linear discriminant analysis to the biochemical and haematological differentiation of opiate addicts from healthy subjects: a case–control study  

Microsoft Academic Search

Objective: Biochemical and haematological parameters of nutritional interest were determined in the serum of opiate addicts in order to compare them with those obtained in healthy subjects. Linear discriminant analysis was applied for the differentiation of the opiate addicts.Subjects: Sera of 106 opiate addicts in detoxification treatment (n=19) or in Methadone Maintenance Treatment Program (MMTP) (n=87) were studied.Design: The determination

J F Díaz-Flores Estévez; F Díaz-Flores Estévez; C Hernández Calzadilla; E M Rodríguez Rodríguez; C Díaz Romero; L Serra-Majem

2004-01-01

182

Effects of transporting bulls at different space allowances on physiological, haematological and immunological responses to a 12-h journey by road  

Microsoft Academic Search

The effects of space allowance during transportation on physiological, haematological and immunological responses in nine-month old bulls (250 kg) were assessed before and after a 12-h road journey. Following transport, animals transported at a spatial allowance of 1.27 m2 had higher (P ? 0.001) non-esterified fatty acid concentrations than control. The stimulated production of interferon-?, in response to concanavalin-A and

B. Earley; E. G. O'Riordan

183

Haematological changes in the three Indian major carps, Catla catla (Hamilton), Labeo rohita (Hamilton) and Cirrhinus mrigala (Hamilton) exposed to acidic and alkaline water pH  

Microsoft Academic Search

The effect of water pH on certain haematological parameters was studied in the three species of Indian major carps, catla (Catla catla), rohu (Labeo rohita) and mrigal (Cirrhinus mrigala) for 21 days by subjecting fingerlings of these fishes to acidic (pH 5.5, 6.5) and alkaline (pH 8.0, 8.5 and 9.0) water, control groups were maintained at neutral pH. A change

P. C. Das; S. Ayyappan; J. K. Jena

2006-01-01

184

Physiological responses to starvation in the European eel ( Anguilla anguilla ): effects on haematological, biochemical, non-specific immune parameters and skin structures  

Microsoft Academic Search

The physiological effects of short-term starvation on some haematological, biochemical and non-specific immune response parameters\\u000a together with the histological structure of the skin, were investigated in the European eel, Anguilla anguilla. Blood haemoglobin and haematocrit, serum glucose and cortisol, hemolysins, haemagglutinins, and lysozyme in the plasma,\\u000a kidney and epidermal extract, were measured in fish after 31, 42 and 58 days of starvation,

G. Caruso; G. Maricchiolo; V. Micale; L. Genovese; R. Caruso; M. G. Denaro

2010-01-01

185

Influence of esterified-glucomannan on performance and organ morphology, serum biochemistry and haematology in broilers exposed to individual and combined mycotoxicosis (aflatoxin, ochratoxin and T-2 toxin)  

Microsoft Academic Search

1. A study was conducted to evaluate the individual and combined effects of aflatoxin B 1 (AF), ochratoxin A (OA) and T-2 toxin (T-2) on performance, organ morphology, serum biochemistry and haematology of broiler chickens and the efficacy of esterified-glucomannan (E-GM), a cell wall derivative of Saccharomyces cerevisiae 1026 in their counteraction. 2. Two dietary inclusion rates of AF (0

M. V. L. N. Raju; G. Devegowda

2000-01-01

186

Inulin and probiotics in newly weaned piglets: effects on intestinal morphology, mRNA expression levels of inflammatory marker genes and haematology  

Microsoft Academic Search

The study aimed at determining the effect of inulin and\\/or a multispecies probiotic formulation on gastrointestinal tract (GIT) morphology, immunological and haematological parameters. Forty-eight newly weaned piglets were assigned to four feeding groups, receiving a standard basal diet (control), supplemented with 0.4% inulin, probiotics (1 · 10 CFU\\/kg as fed, enterococci, lactobacilli, bifidobacteria) or a combination of both (synbiotic). After four weeks

Christiane Mair; Christian Plitzner; Michael W. Pfaffl; Karl Schedle; Heinrich H. D. Meyer; Wilhelm Windisch

2010-01-01

187

Cavernosal arterial insufficiency is a major component of erectile dysfunction in some recipients of high-dose chemotherapy\\/chemo-radiotherapy for haematological malignancies  

Microsoft Academic Search

We studied 24 male patients aged 26–62 years (median 41) prospectively presenting over a 5 year period with clinical features of hypogonadism and erectile dysfunction (ED), who had been treated with autologous or allogeneic bone marrow\\/stem cell transplant for a variety of haematological malignancies and had received either high-dose chemotherapy or high-dose chemotherapy combined with total body irradiation (TBI). Ten

R Chatterjee; HO Andrews; HH McGarrigle; PD Kottaridis; WR Lees; S Mackinnon; DJ Ralph; AH Goldstone

2000-01-01

188

Loci Associated with N-Glycosylation of Human Immunoglobulin G Show Pleiotropy with Autoimmune Diseases and Haematological Cancers  

PubMed Central

Glycosylation of immunoglobulin G (IgG) influences IgG effector function by modulating binding to Fc receptors. To identify genetic loci associated with IgG glycosylation, we quantitated N-linked IgG glycans using two approaches. After isolating IgG from human plasma, we performed 77 quantitative measurements of N-glycosylation using ultra-performance liquid chromatography (UPLC) in 2,247 individuals from four European discovery populations. In parallel, we measured IgG N-glycans using MALDI-TOF mass spectrometry (MS) in a replication cohort of 1,848 Europeans. Meta-analysis of genome-wide association study (GWAS) results identified 9 genome-wide significant loci (P<2.27×10?9) in the discovery analysis and two of the same loci (B4GALT1 and MGAT3) in the replication cohort. Four loci contained genes encoding glycosyltransferases (ST6GAL1, B4GALT1, FUT8, and MGAT3), while the remaining 5 contained genes that have not been previously implicated in protein glycosylation (IKZF1, IL6ST-ANKRD55, ABCF2-SMARCD3, SUV420H1, and SMARCB1-DERL3). However, most of them have been strongly associated with autoimmune and inflammatory conditions (e.g., systemic lupus erythematosus, rheumatoid arthritis, ulcerative colitis, Crohn's disease, diabetes type 1, multiple sclerosis, Graves' disease, celiac disease, nodular sclerosis) and/or haematological cancers (acute lymphoblastic leukaemia, Hodgkin lymphoma, and multiple myeloma). Follow-up functional experiments in haplodeficient Ikzf1 knock-out mice showed the same general pattern of changes in IgG glycosylation as identified in the meta-analysis. As IKZF1 was associated with multiple IgG N-glycan traits, we explored biomarker potential of affected N-glycans in 101 cases with SLE and 183 matched controls and demonstrated substantial discriminative power in a ROC-curve analysis (area under the curve?=?0.842). Our study shows that it is possible to identify new loci that control glycosylation of a single plasma protein using GWAS. The results may also provide an explanation for the reported pleiotropy and antagonistic effects of loci involved in autoimmune diseases and haematological cancer.

Muzinic, Ana; Novokmet, Mislav; Polasek, Ozren; Gornik, Olga; Kristic, Jasminka; Keser, Toma; Vitart, Veronique; Scheijen, Blanca; Uh, Hae-Won; Molokhia, Mariam; Patrick, Alan Leslie; McKeigue, Paul; Kolcic, Ivana; Lukic, Ivan Kresimir; Swann, Olivia; van Leeuwen, Frank N.; Ruhaak, L. Renee; Houwing-Duistermaat, Jeanine J.; Slagboom, P. Eline; Beekman, Marian; de Craen, Anton J. M.; Deelder, Andre M.; Zeng, Qiang; Wang, Wei; Hastie, Nicholas D.; Gyllensten, Ulf; Wilson, James F.; Wuhrer, Manfred; Wright, Alan F.; Rudan, Igor

2013-01-01

189

A Study on Course of Infection and Haematological Changes in falciparum-Infected in Comparison with Artemisinin(s)-Treated Mice.  

PubMed

To find out the efficacy and effect of artemisinin derivatives on haematological indices, C57BL/6J mice were challenged with Plasmodium falciparum and treated with therapeutic doses of AS, AE, and AL. Course of infection was studied in the infected and treated groups up to day 42. Peak level of parasitaemia (38%) was observed on day 11 in infected group. Haematological indices indicated significant (P < 0.05) decrease in RBC, WBC, haemoglobin, packed cell volume, mean cell volume, and platelet counts in infected mice. But all the parameters were restored to normal values, and significant (P < 0.05) changes were observed in all drug-treated groups. Insignificant changes were observed for MCHC (P > 0.05) in all drug-treated groups. Percent of peak parasitaemia was much reduced in AL- (3.2% on day 3) treated group in comparison with AE- (2.4% on day 4) and AS- (4% on day 2) treated groups. Parasites were completely cleared on day 6 in AS group, day 5 in AE group, and day 4 in AL group. Hence, our results strongly support that combination therapy has high efficacy rates than monotherapy. No adverse effects were observed on haematological parameters when animals were treated with therapeutic dosages. PMID:23841019

Kuthala, Kalyan Kumar; Meka, Sowjanya; Kanikaram, Sunita

2013-06-11

190

A Study on Course of Infection and Haematological Changes in falciparum-Infected in Comparison with Artemisinin(s)-Treated Mice  

PubMed Central

To find out the efficacy and effect of artemisinin derivatives on haematological indices, C57BL/6J mice were challenged with Plasmodium falciparum and treated with therapeutic doses of AS, AE, and AL. Course of infection was studied in the infected and treated groups up to day 42. Peak level of parasitaemia (38%) was observed on day 11 in infected group. Haematological indices indicated significant (P < 0.05) decrease in RBC, WBC, haemoglobin, packed cell volume, mean cell volume, and platelet counts in infected mice. But all the parameters were restored to normal values, and significant (P < 0.05) changes were observed in all drug-treated groups. Insignificant changes were observed for MCHC (P > 0.05) in all drug-treated groups. Percent of peak parasitaemia was much reduced in AL- (3.2% on day 3) treated group in comparison with AE- (2.4% on day 4) and AS- (4% on day 2) treated groups. Parasites were completely cleared on day 6 in AS group, day 5 in AE group, and day 4 in AL group. Hence, our results strongly support that combination therapy has high efficacy rates than monotherapy. No adverse effects were observed on haematological parameters when animals were treated with therapeutic dosages.

Kuthala, Kalyan Kumar; Meka, Sowjanya; Kanikaram, Sunita

2013-01-01

191

Effects of urea-treated fibrous diets on nutrient intake, digestibility, performance and haematological parameters of Yankasa rams.  

PubMed

An experiment was conducted to investigate the effects of urea-treated fibrous diets on the intake, nutrient digestibility, performance and haematological parameters of Yankasa rams. A total of 48 Yankasa rams (BW 10.00 ± 1.50 kg; 6-8 months old) were allocated into four treatment groups in a completely randomised design (12 rams per treatment). Animals were placed on complete rations of yam peels, maize bran and rice husk treated with 0, 1.0, 1.5 or 2.0% urea. The experiment lasted for 18 weeks. Yankasa rams fed with urea-treated diets had higher feed intake (949.88 and 938.04 g/day for U?? and U??, respectively), daily weight gain (227.67 and 181.00 g/day for U?? and U??, respectively) and better feed conversion ratio (4.17 and 5.18 for U?? and U??, respectively). Rams on urea-treated diets had higher haemoglobin and red blood cell contents and higher weight gains, indicating that urea treatment enhanced nutrient supply and utilisation at the tissue level. It was concluded that urea treatment of fibrous farm by-products is a promising feeding strategy especially during the dry season when there is scarcity of high-quality forages. Addition of 1.5% urea to roughage diets and farm by-products to form a total mixed ration may preclude the search for supplements. PMID:23397549

Alabi, John Olushola; Arigbede, Moses; Ng'ambi, Jones; Norris, David; Shiawoya, Emma; Onyekachi, Sunday

2013-02-09

192

Dietary supplementation with chitin and chitosan on haematology and innate immune response in Epinephelus bruneus against Philasterides dicentrarchi.  

PubMed

The present study investigated the effect of 1.0% chitin and chitosan supplementation diets on haematology and immune response in Kelp grouper, Epinephelus bruneus against protozoan parasite, Philasterides dicentrarchi. The red blood cells (RBC), white blood cells (WBC), haemoglobin levels, lymphocytes, monocytes, and neutrophils significantly increased in kelp grouper fed with chitin or chitosan enriched diets against P. dicentrarchi. The mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), and thrombocytes did not significantly change against pathogen. The phagocytic activity, respiratory burst activity, complement activity, antiprotease activity, and ?2-macroglobulin were significantly enhanced in fish fed with 1% chitin and chitosan diet on weeks 2 and 4. The lysozyme activity, total protein, and myeloperoxidase activity significantly increased in fish fed with chitin or chitosan supplementation diet from weeks 1 to 4 against pathogen. The cumulative mortality was found low in fish fed with chitin and chitosan enriched diets than those of control against pathogen. The present study suggests that supplementation of 1.0% chitin or chitosan in diets positively enhances immune response and affords disease resistance in kelp grouper, E. bruneus against P. dicentrarchi infection. PMID:22475776

Harikrishnan, Ramasamy; Kim, Ju-Sang; Balasundaram, Chellam; Heo, Moon-Soo

2012-03-27

193

Real-world costs of autologous and allogeneic stem cell transplantations for haematological diseases: a multicentre study.  

PubMed

Haematopoietic stem cell transplantation (SCT) is an expensive lifesaving procedure, which is increasingly performed in patients with haematological diseases. Developments in the protocol for SCT have resulted in cost estimates that require updating. We aimed to calculate actual costs for SCT and to identify major cost drivers by means of a daily practice cost study. We randomly selected 191 patients, treated at three university hospitals, who underwent an autologous (auto) SCT or allogeneic (allo) SCT in 2007, 2008 or 2009. Allo-SCT included sibling donors, matched unrelated donors (MUD) and umbilical cord blood (UCB). Resource use was collected from the hospital registration systems and medical files. The total costs included selection and harvesting of stem cells, transplantation and 1-year follow-up. The average costs per patient were 45,670 € for auto-SCT and 101,919 € for sibling allo-SCT. The costs of transplantations from unrelated donors were much higher: 171,478 € for allo-SCT-MUD and 254,689 € for allo-SCT-UCB. Hospital inpatient days together with laboratory and other activities were the main cost drivers across all types of SCT. Besides, donor search costs were a large cost component in allo-SCT-sib (18 %) and allo-SCT-MUD (12 %). Real-world costs were above routine reimbursement and appropriate financing is necessary to guarantee the continuation of SCT. The costs calculated in this study provide reliable up-to-date input for cost-effectiveness studies and budget revision. PMID:22864761

Blommestein, H M; Verelst, S G R; Huijgens, P C; Blijlevens, N M A; Cornelissen, J J; Uyl-de Groot, C A

2012-08-05

194

Epidemiology and sites of involvement of invasive fungal infections in patients with haematological malignancies: a 20-year autopsy study.  

PubMed

Autopsy studies remain an essential tool for understanding the patterns of fungal disease not detected ante mortem with current diagnostic approaches. We collected data concerning the microbiological trends, patient clinical characteristics and sites of involvement for invasive fungal infections (IFIs) identified at autopsy in a single large cancer treatment centre over a 20-year period (1989-2008). The autopsy rate and IFI prevalence both declined significantly during the study period. The prevalence of Aspergillus spp. decreased significantly from the first 15 years of the study (from 0.12 to 0.14 cases per 100 autopsies to 0.07 in 2004-2008; P = 0.04), with only Mucorales accounting for a greater proportion of IFIs over the duration of the study period (0.06 to 0.2 cases per 100 autopsies, P = 0.04). After 2003, moulds accounted for the majority of infections identified at autopsy in the spleen, kidney, heart and gastrointestinal tract. Despite a trend of decreasing prevalence from 1989 to 2004, invasive candidiasis increased in prevalence during later periods 2004-2008 (0.02-0.05 per 100 autopsies) with decreasing kidney, heart and spleen involvement. Despite a declining autopsy rate, these data suggest a decreasing prevalence overall of IFIs with changing patterns of dissemination in patients with haematological malignancies. PMID:23551865

Lewis, Russell E; Cahyame-Zuniga, Lizebeth; Leventakos, Konstantinos; Chamilos, Georgios; Ben-Ami, Ronen; Tamboli, Pheroze; Tarrand, Jeffrey; Bodey, Gerald P; Luna, Mario; Kontoyiannis, Dimitrios P

2013-04-04

195

Haematological, biochemical and inflammatory parameters in inactive Behçet's disease. Its association with red blood cell distribution width.  

PubMed

Red blood cell distribution width (RDW) has been shown to be associated with disease activity in several inflammatory disorders. However only one study to show this has been conducted in patients with Behçet's disease (BD). The aim of the present study was to analyse the association of RDW with BD and its main complications; i.e.; thrombosis and posterior uveitis. A second aim was to analyse the possible correlation between RDW and both haematological and inflammatory parameters. Eighty-nine patients with BD (48 males/41 females) and 94 controls (49 males/45 females) were included in the study. Patients were in an inactive phase of the disease, showing only minimum activity. RDW was statistically higher in patients than in controls (14.02 ± 1.32 vs. 13.15 ± 0.75; p < 0.001) as were CRP, fibrinogen, leucocytes and neutrophils (p < 0.001). No differences in haematimetric indices (MCV, MCH, MCHC) were observed (p > 0.05). RDW correlated negatively with haemoglobin, MCH and MCHC (p < 0.05), and directly with homocysteine (p < 0.01). No correlation was found between RDW and the several inflammatory parameters analysed (p > 0.05). The multivariate regression analysis revealed that haemoglobin and homocysteine were independent predictors of RDW (beta coefficient: -0.310; p = 0.003, beta coefficient: 0.379; p < 0.001, respectively). RDW >14 was associated with neither thrombosis nor uveitis (p = 0.935; p = 0.553, respectively). Our results indicate that BD patients show increased RDW when compared with controls. This increase seems to be related with haematimetric indices and with homocysteine levels. Lack of correlation with inflammatory markers may be due to the fact that patients were in an inactive phase of the disease. PMID:23719420

Vayá, Amparo; Rivera, Leonor; Todolí, José; Hernandez, Jose Luis; Laiz, Begońa; Ricart, José M

2013-05-29

196

Variation in haematological parameters in children less than five years of age with asymptomatic Plasmodium infection: implication for malaria field studies.  

PubMed

During the season of high malaria transmission, most children are infected by Plasmodium, which targets red blood cells (RBCs), affecting haematological parameters. To describe these variations, we examined the haematological profiles of two groups of children living in a malaria-endemic area. A cross-sectional survey was conducted at the peak of the malaria transmission season in a rural area of Burkina Faso. After informed consent and clinical examination, blood samples were obtained from the participants for malaria diagnosis and a full blood count. Of the 414 children included in the analysis, 192 were not infected with Plasmodium, whereas 222 were asymptomatic carriers of Plasmodium infection. The mean age of the infected children was 41.8 months (range of 26.4-57.2) compared to 38.8 months (range of 22.4-55.2) for the control group (p = 0.06). The asymptomatic infected children tended to have a significantly lower mean haemoglobin level (10.8 g/dL vs. 10.4 g/dL; p < 0.001), mean lymphocyte count (4592/µL vs. 5141/µL; p = 0.004), mean platelet count (266 x 10ł/µL vs. 385 x 10ł/µL; p < 0.001) and mean RBC count (4.388 x 10(6)/µL vs. 4.158 x 10(6)/µL; p < 0.001) and a higher mean monocyte count (1403/µL vs. 1192/µL; p < 0.001) compared to the control group. Special attention should be applied when interpreting haematological parameters and evaluating immune responses in asymptomatic infected children living in malaria-endemic areas and enrolled in vaccine trials. PMID:23903982

Gansane, Adama; Ouedraogo, Issa Nebie; Henry, Noelie Bere; Soulama, Issiaka; Ouedraogo, Esperance; Yaro, Jean-Baptiste; Diarra, Amidou; Benjamin, Sombie; Konate, Amadou Tidiani; Tiono, Alfred; Sirima, Sodiomon Bienvenu

2013-08-01

197

Mortality, morbidity, and haematological results from a cohort of long-term workers involved in 1,3-butadiene monomer production.  

PubMed Central

A retrospective mortality analysis and prospective morbidity and haematological analyses were performed for Shell Deer Park Manufacturing Complex (DPMC) male employees who worked in jobs with potential exposure to 1,3-butadiene from 1948 to 1989. 614 employees qualified for the mortality study (1948-89), 438 of those were still employed during the period of the morbidity study (1982-9), and 429 of those had haematological data available for analysis. Industrial hygiene data from 1979 to 1992 showed that most butadiene exposures did not exceed 10 ppm (eight-hour time weighted average (8 hour TWA)), and most were below 1 ppm, with an arithmetic mean of 3.5 ppm. 24 deaths occurred during the mortality study period. For all causes of death, the standardised mortality ratio (SMR) was 48 (95% confidence interval (95% CI) = 31-72), and the all cancer SMR was 34 (95% CI = 9-87). There were only two deaths due to lung cancer (SMR 42, 95% CI = 5-151) and none due to lymphohaematopoietic cancer (expected = 1.2). Morbidity (illness absence) events of six days or more for the 438 butadiene employees were compared with the rest of the complex. No cause of morbidity was in excess for this group; the all cause standardised morbidity ratio (SMbR) was 85 (95% CI = 77-93) and the all neoplasms SMbR was 51 (95% CI = 22-100). Haematological results for the 429 with laboratory data were compared with results for the rest of the complex. No significant differences occurred between the two groups and the distributions of results between butadiene and non-butadiene groups were virtually identical. These results suggest that butadiene exposures at concentrations common at DPMC in the past 10-20 years do not pose a health hazard to employees.

Cowles, S R; Tsai, S P; Snyder, P J; Ross, C E

1994-01-01

198

Variations in Haematological Parameters and Erythrocyte Osmotic Fragility of Pigs during Hot-Dry and Harmattan Season in Northern Guinea Savanna Zone of Nigeria.  

PubMed

Experiments were performed with the aim of investigating the effect of season on haematological parameters and erythrocyte osmotic fragility (EOF) of pigs. A total of 23 local pigs including males, non-pregnant and non-nursing females, aged 9 to 12 months were used for the study, ten animals were used during the hot-dry season and thirteen during the harmattan season. Blood sample was taken from each animal for the determination of EOF and other haematological parameters as well as total protein. The PCV value of 39.7±1.9 % obtained during the hot-dry season was significantly higher than 32.00 ± 0.9 % obtained during the harmattan season. Total leucocyte count of 18,836.5±1727.1 obtained during the harmattan season was higher than the value 15,920.00±1119.1 recorded during the hot-dry season. The neutrophil:lymphocyte ratio value was significantly higher during the harmattan season, with a value of 0.61±0.0 than the recorded value of 0.43±0.0 during the hot-dry season. The percentage haemolysis values obtained during the harmattan season at NaCl concentration of 0.5-0.9 % with a value of 92.03±0.02 % respectively were significantly higher than those recorded during the hot-dry season. In conclusion, the haematological values showed that harmattan season was more stressful to pigs than the hot-dry season in the Northern Guinea Savanna zone of Nigeria. PMID:22547178

Adenkola, A Y; Ayo, J O; Asala, O O

2011-12-20

199

White blood cell count in women: relation to inflammatory biomarkers, haematological profiles, visceral adiposity, and other cardiovascular risk factors.  

PubMed

The role of white blood cell (WBC) count in pathogenesis of diabetes, cardiovascular disease, and obesity-related disorders has been reported earlier. Recent studies revealed that higher WBC contributes to atherosclerotic progression and impaired fasting glucose. However, it is unknown whether variations in WBC and haematologic profiles can occur in healthy obese individuals. The aim of this study is to further evaluate the influence of obesity on WBC count, inflammatory biomarkers, and metabolic risk factors in healthy women to establish a relationship among variables analyzed. The sample of the present study consisted of 84 healthy women with mean age of 35.56 +/- 6.83 years. They were categorized into two groups based on their body mass index (BMI): obese group with BMI > 30 kg/m2 and non-obese group with BMI < 30 kg/m2. We evaluated the relationship between WBC and platelet count (PLT) with serum interleukin 6 (IL-6), C-reactive protein (CRP), angiotensin pi (Ang pi), body fat percentage (BF %), waist-circumference (WC), and lipid profile. WBC, PLT, CRP, and IL-6 in obese subjects were significantly higher than in non-obese subjects (p < 0.05). The mean WBC count in obese subjects was 6.4 +/- 0.3 (x10(9)/L) compared to 4.4 +/- 0.3 (x10(9)/L) in non-obese subjects (p = 0.035). WBC correlated with BF% (r = 0.31, p = 0.004), CRP (r = 0.25, P = 0.03), WC (r = 0.22, p = 0.04), angiotensin 11 (r = 0.24, p = 0.03), triglyceride (r = 0.24, p = 0.03), and atherogenic index of plasma (AIP) levels (r = 0.3, p = 0.028) but not with IL-6. Platelet count was also associated with WC and waist-to-hip ratio (p < 0.05). Haemoglobin and haematocrit were in consistent relationship with LDL-cholesterol (p < 0.05). In conclusion, obesity was associated with higher WBC count and inflammatory parameters. There was also a positive relationship between WBC count and several inflammatory and metabolic risk factors in healthy women. PMID:23617205

Farhangi, Mahdieh Abbasalizad; Keshavarz, Seyyed-Ali; Eshraghian, Mohammadreza; Ostadrahimi, Alireza; Saboor-Yaraghi, Ali-Akbar

2013-03-01

200

White Blood Cell Count in Women: Relation to Inflammatory Biomarkers, Haematological Profiles, Visceral Adiposity, and Other Cardiovascular Risk Factors  

PubMed Central

The role of white blood cell (WBC) count in pathogenesis of diabetes, cardiovascular disease, and obesity-related disorders has been reported earlier. Recent studies revealed that higher WBC contributes to atherosclerotic progression and impaired fasting glucose. However, it is unknown whether variations in WBC and haematologic profiles can occur in healthy obese individuals. The aim of this study is to further evaluate the influence of obesity on WBC count, inflammatory biomarkers, and metabolic risk factors in healthy women to establish a relationship among variables analyzed. The sample of the present study consisted of 84 healthy women with mean age of 35.56±6.83 years. They were categorized into two groups based on their body mass index (BMI): obese group with BMI >30 kg/m2 and non-obese group with BMI <30 kg/m2. We evaluated the relationship between WBC and platelet count (PLT) with serum interleukin 6 (IL-6), C-reactive protein (CRP), angiotensin ? (Ang ?), body fat percentage (BF %), waist-circumference (WC), and lipid profile. WBC, PLT, CRP, and IL-6 in obese subjects were significantly higher than in non-obese subjects (p< 0.05). The mean WBC count in obese subjects was 6.4±0.3 (×109/L) compared to 4.4±0.3 (×109/L) in non-obese subjects (p=0.035). WBC correlated with BF% (r=0.31, p=0.004), CRP (r=0.25, P=0.03), WC (r=0.22, p=0.04), angiotensin ? (r=0.24, p=0.03), triglyceride (r=0.24, p=0.03), and atherogenic index of plasma (AIP) levels (r=0.3, p=0.028) but not with IL-6. Platelet count was also associated with WC and waist-to-hip ratio (p<0.05). Haemoglobin and haematocrit were in consistent relationship with LDL-cholesterol (p<0.05). In conclusion, obesity was associated with higher WBC count and inflammatory parameters. There was also a positive relationship between WBC count and several inflammatory and metabolic risk factors in healthy women.

Farhangi, Mahdieh Abbasalizad; Keshavarz, Seyyed-Ali; Eshraghian, Mohammadreza; Ostadrahimi, Alireza

2013-01-01

201

Gastrointestinal nematode burden in working equids from humid tropical areas of central Veracruz, Mexico, and its relationship with body condition and haematological values.  

PubMed

The east coast of Veracruz, Mexico, has an important equine population used for working in rural production systems. The objectives of this study were (1) to calculate the prevalence of tropical working equids (donkeys, mules and horses) infected with gastrointestinal nematodes (GINs) and the GINs involved, and (2) to measure the body condition score (BCS) and haematological values for each working equid and its relationship with faecal worm egg count (EPG). One hundred and forty working equids were randomly selected from five different villages along the central coast of the state of Veracruz and faecal and blood samples were obtained from each animal. Gastrointestinal parasite burdens were determined using the McMaster technique. Packed cell volume, total plasma proteins, red blood cell count and white blood cell count were measured from each blood sample. Prevalence of infected equids was higher than 90 %. Mules had the highest median faecal worm egg counts (875 EPG), followed by horses and donkeys with 400 EPG. There was no correlation between EPG and BCS or haematological values (p > 0.05). Results suggest that despite the high prevalence and parasite burdens, equids involved in this trial are not being seriously affected. This study provides information which might help in designing future strategies to control nematode infections in working equids in the Mexican tropics; more emphasis should be placed on other inputs (nutrition perhaps), with individual anthelminthic treatment to those animals with the highest EPG or when signs present themselves. PMID:22992947

Valdéz-Cruz, Maura Pilar; Hernández-Gil, Mariano; Galindo-Rodríguez, Leticia; Alonso-Díaz, Miguel Angel

2012-09-20

202

Effects of prolonged strenuous exercise (marathon running) on biochemical and haematological markers used in the investigation of patients in the emergency department  

PubMed Central

Objectives: To investigate the effects of strenuous exercise on commonly used biochemical and haematological variables in subjects running the 2002 London marathon. Methods: 34 healthy volunteers (7 female, 27 male) were recruited for the study. Blood was taken before the start (at registration) and immediately after completion of the marathon. Samples were analysed for urea and electrolytes, liver function tests, creatine kinase (CK), CK-MB isoenzyme, myoglobin, troponin I, full blood count, a clotting screen, and D-dimers. The results before and after exercise were compared. Pearson's correlation coefficients were calculated for all variables. Results: Significant increases were found in CK, CK-MB, aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and myoglobin following the marathon. However, there was no significant change in the level of troponin I. There was also evidence of activation of the coagulation and fibrinolytic cascades following the marathon, with a reduction in activated partial thromboplastin time, a reduction in fibrinogen, and an increase in D-dimers. Conclusions: The results confirm previous individual studies on marathon running and the biochemical and haematological tests routinely carried out in hospital. These are affected by prolonged exercise, and "abnormal" results in these tests may be normal after prolonged exercise and therefore not diagnostic of a disease process. The results of investigations in patients who have been exercising should be interpreted with caution.

Smith, J; Garbutt, G; Lopes, P; Pedoe, D

2004-01-01

203

[Proteins in haematology].  

PubMed

The introduction of monoclonal antibodies has been a milestone in the treatment of hematologic neoplasms. The CD20 antibody rituximab has been a trailblazer and represents meanwhile a fixed combination partner of different first- and second-line chemotherapies in CD20-positive B-cell-non-Hodgkin's lymphoma (NHL). Rituximab maintenance is established in follicular lymphoma. The drug is also used for the treatment of non-malignant hematologic diseases (i.e. immunthrombocytopenia). Rituximab was followed by the CD52 antibody alemtuzumab and recently by the CD20 antibody ofatumumab for the therapy of CLL and the radioimmunconjugate ibritumomab-tiuxetan indicated for the treatment of refractory or relapsed CD20-positive follicular lymphoma or as consolidation after induction chemotherapy. In the near future, approval of several new antibodies is expected. For the treatment of refractory or relapsed Hodgkin's lymphoma and relapsed systemic anaplastic large cell lymphoma, the CD30 antibody brentuximab vedotin has been shown to be a highly active new treatment option. PMID:22045523

Clarenbach, Ricarda E; Mey, Ulrich

2011-11-01

204

Assessment of chronic administration of aloe vera gel on haematology, plasma biochemistry, lipid profiles and erythrocyte osmotic resistance in wistar rats.  

PubMed

The study was designed to investigate the effects of chronic administration of Aloe vera gel extract on markers of hepatic damage, lipid profiles and erythrocyte osmotic fragility using the Wistar rats. Forty male Wistar rats divided into four groups of ten rats per group were used in the study. Group I which served as the control received 0.9% physiological saline while those in groups II, III and IV received Aloe vera gel (100, 250 and 500mg/kg), respectively, for four weeks. There was significant increase in the haemoglobin concentration while the PCV, RBC count, MCH and MCHC though showed some marginal increases but the increases were not significant in all the treated rats. No significant change was also observed in the erythrocyte osmotic fragility. However, there were significant reductions in plasma ALT, AST and ALP levels in animals that received the gel compared with the control while the plasma albumin and total protein values were higher than those of the control. All the animals that received the gel also showed significant reduction in plasma total cholesterol, triglycerides and LDL-cholesterol ratio compared with the control. In a similar manner, those animals that were administered with 500mg/kg gel had significantly higher HDL-cholesterol ratio than those of the control. This study showed that, chronic administration of Aloe vera gel extract had no significant effects on the haematological parameters of the rats and did not affect erythrocyte osmotic resistance. It however showed some cholesterol lowering action.keywords: Aloe vera, Haematology, Osmotic fragility, Plasma biochemistry. PMID:22314947

Iji, O T; Oyagbemi, A A; Azeez, O I

2010-11-28

205

Effects of Exercise on Haematologic Parameters, Serum Iron, Serum Ferritin, Red Cell 2,3Diphosphoglycerate and Creatine Contents, and Serum Erythropoietin in Long-Distance Runners during Basal Training  

Microsoft Academic Search

We studied 18 well-trained male long-distance runners during the basal training. Haematologic parameters, serum iron and ferritin, red cell 2,3-diphosphoglycerate (2,3-DPG) and creatine contents, serum erythropoietin were investigated before and after the daily training and were compared with a group of healthy untrained controls. Red blood cell parameters did not change with the training, even though they were significantly lower

G. Ricci; M. Masotti; De Paoli Vitali; M. Vedovato; G. Z. Zanotti

1988-01-01

206

The effect of the combination of acids and tannin in diet on the performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs  

PubMed Central

Background The abolition of in-feed antibiotics or chemotherapeutics as growth promoters have stimulated the swine industry to look for alternatives such as organic acids, botanicals, probiotics and tannin. The objective of the present study was to compare the effects of a combination of acids and tannin with diet with organic acids and diet without growth promoters on the growth performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs. Tannin is more natural and cheaper but possibly with the same effectiveness as organic acids with regard to growth performance. Methods Thirty-six 7 week old grower pigs, divided into three equal groups, were used in a three week feeding trial. Group I was fed basal diet, group II basal diet with added organic acids and group III basal diet with added organic and inorganic acids and tannin. Pigs were weighed before and after feeding and observed daily. Blood was collected before and after the feeding trial for the determination of selected biochemical, haematological and antioxidant enzyme parameters. One-way ANOVA was used to assess any diet related changes of all the parameters. Paired t-test was used to evaluate changes of blood parameters individually in each group of growers before and after feeding. Results No clinical health problems related to diet were noted during the three week feeding trial. The average daily gain (ADG) and selected blood parameters were not affected by the addition to basal diet of either acids and tannin or of organic acids alone. Selected blood parameters remained within the reference range before and after the feeding trial, with the exception of total serum proteins that were below the lower value of reference range at both times. The significant changes (paired t-test) observed in individual groups before and after the feeding trial are related to the growth of pigs. Conclusion Diet with acids and tannin did not improve the growth performance of grower pigs but had no deleterious effects on selected blood parameters. The possibility of beneficial effects of adding acids and tannin in diets on growth performance over a longer period, however, could not be excluded.

2010-01-01

207

Influence of selected Indian immunostimulant herbs against white spot syndrome virus (WSSV) infection in black tiger shrimp, Penaeus monodon with reference to haematological, biochemical and immunological changes.  

PubMed

Immunostimulants are the substances, which enhance the non-specific defence mechanism and provide resistance against the invading pathogenic micro-organism. In order to increase the immunity of shrimps against the WSSV, the methanolic extracts of five different herbal medicinal plants like Cyanodon dactylon, Aegle marmelos, Tinospora cordifolia, Picrorhiza kurooa and Eclipta alba were selected and mixed thoroughly in equal proportion. The mixed extract was supplemented with various concentrations viz. 100 (A), 200 (B), 400 (C), and 800 (D) mgkg(-1) through artificial diets individually. The prepared diets (A-D) were fed individually to WSSV free healthy shrimp Penaeus monodon with an average weight of 8.0+/-0.5g for 25 days. Control diet (E), devoid of herbal extract was also fed to shrimps simultaneously. After 25 days of feeding experiment, the shrimps were challenged with WSSV, which were isolated and propagated from the infected crustaceans. The shrimps succumbed to death within 7 days when fed on no herbal immunostimulant diet (E). Among the different concentrations of herbal immunostimulant supplemented diets, the shrimps fed on diet D (800mgkg(-1)) significantly (P<0.0001) had more survival (74%) and reduction in the viral load. Also the better performance of haematological, biochemical and immunological parameters was found in the immunostimulant incorporated diets fed shrimps. The present work revealed that the application of herbal immunostimulants will be effective against shrimp viral pathogenesis and they can be recommended for shrimp culture. PMID:16698283

Citarasu, Thavasimuthu; Sivaram, Veeramani; Immanuel, Grasian; Rout, Namita; Murugan, Vadivel

2006-05-12

208

Modifications of haematology analyzers to improve cell counting and leukocyte differentiating in cerebrospinal fluid controls of the Joint German Society for Clinical Chemistry and Laboratory Medicine.  

PubMed

Flow cytometry (FCM) is used with haematology analyzers (HAs) to count cells and differentiate leukocytes in cerebrospinal fluid (CSF). To evaluate the FCM techniques of HAs, 10 external DGKL trials with CSF controls were carried out in 2004 to 2008. Eight single platform HAs with and without CSF equipment were evaluated with living blood leukocytes and erythrocytes in CSF like DGKL controls: Coulter (LH750,755), Abbott CD3200, CD3500, CD3700, CD4000, Sapphire, ADVIA 120(R) CSF assay, and Sysmex XE-2100(R). Results were compared with visual counting of native cells in Fuchs-Rosenthal chamber, unstained, and absolute values of leukocyte differentiation, assayed by dual platform analysis with immune-FCM (FACSCalibur, CD45, CD14) and the chamber counts. Reference values X were compared with HA values Y by statistical evaluation with Passing/Bablock (P/B) linear regression analysis to reveal conformity of both methods. The HAs, studied, produced no valid results with DGKL CSF controls, because P/B regression revealed no conformity with the reference values due to:-blank problems with impedance analysis,-leukocyte loss with preanalytical erythrocyte lysis procedures, especially of monocytes,-inaccurate results with ADVIA cell sphering and cell differentiation with algorithms and enzyme activities (e.g., peroxidase). HA techniques have to be improved, e.g., using no erythrocyte lysis and CSF adequate techniques, to examine CSF samples precise and accurate. PMID:19526484

Kleine, Tilmann O; Nebe, C Thomas; Löwer, Christa; Lehmitz, Reinhard; Kruse, Rolf; Geilenkeuser, Wolf-Jochen; Dorn-Beineke, Alexandra

2009-08-01

209

Pandemic A/H1N1/2009 influenza in a paediatric haematology and oncology unit: successful management of a sudden outbreak.  

PubMed

Viral respiratory infections are potentially life-threatening among children treated for cancer. We report a nosocomial outbreak of six cases of pandemic influenza A/H1N1/2009 on a paediatric haematology and oncology ward. Three patients developed pneumonia and two of them sustained haemodynamic collapse. The source was probably a relative of the first infected patient. The outbreak was probably spread by cross-infection between patients during communal activities. A few days' delay in identifying the outbreak promoted spread of the influenza. Infection control measures included the use of oral oseltamivir treatment for all hospitalised patients, isolation of the infected patients, strict personal protective controls and a restriction on visitors. No new cases occurred after implementation of these containment measures. At the time when the outbreak was identified, all the patients were already isolated for other reasons. We conclude that A/H1N1/2009 influenza may spread rapidly and cause severe infection in paediatric cancer patients but can be efficiently contained. Identification of isolated or clustered cases should lead to the rapid implementation of appropriate infection control measures. PMID:21783276

Buchbinder, N; Dumesnil, C; Pinquier, D; Merle, V; Filhon, B; Schneider, P; Vannier, J P

2011-07-23

210

Central venous catheter-associated bloodstream infection and colonisation of insertion site and catheter tip. What are the rates and risk factors in haematology patients?  

PubMed

Skin colonisation is an important source for central venous catheter (CVC) colonisation and infection. This study intended to identify risk factors for skin colonisation prior to CVC placement (baseline colonisation) and within 10 days after CVC insertion (subsequent colonisation), for CVC-tip colonisation and for bloodstream infection (BSI). Within a randomised clinical trial, data of 219 patients with haematological malignancies and inserted CVC (with a total of 5,501 CVC-days and 4,275 days at risk) in two university hospitals were analysed. Quantitative skin cultures were obtained from the insertion site before CVC placement and at regular intervals afterwards. CVC-tip cultures were taken on CVC removal and data collection was performed. Statistical analysis included linear and logistic regression models. Age was an independent risk factor for colonisation prior to CVC placement (baseline colonisation). Independent risk factors for subsequent colonisation were baseline colonisation and male gender. High level of subsequent skin colonisation at the insertion site was a predictor of CVC-tip colonisation, and a predictor of BSI. High level of skin colonisation predicts catheter tip colonisation and possibly subsequent infection. Sustained reduction of bacterial growth at the CVC insertion site is therefore indispensable. Male patients are at particular risk for skin colonisation and may be a target population for additional insertion-site care before and during catheterisation. PMID:20532506

Luft, Dirk; Schmoor, Claudia; Wilson, Christine; Widmer, Andreas F; Bertz, Hartmut; Frei, Reno; Heim, Dominik; Dettenkofer, Markus

2010-06-08

211

Information for decision makers at hospital laboratory level: an example of a graphical method of representing costs and effects for a replacement automated technology in a haematology laboratory.  

PubMed

The costs and effects on patient management following the introduction of a fully automated blood count analyser have been evaluated for a hospital haematology laboratory in England, during 1985. In the case of the relatively high throughput observed in the laboratory studied, the automated technology was judged to be cheaper if considering laboratory-centred costs alone. But this was no longer necessarily the case once wider costs, including extra medical workload generated for hospital and community physicians, were included. Effects on patient management were measured for all types of patient, including hospital in-patients, out-patients and general practice patients. A graphical method allowing comparison of the costs and effects of the replacement automated system, termed a Technology MAP (Management Awareness Profile) is presented in the paper. MAPs provide a flexible information display which can be tailored to an individual laboratory's situation producing an overall picture of expected costs and effects on patient management following the introduction of a new technology--a full value-in-use assessment. The authors argue that there is a need for a central source of such value-in-use assessments, especially for new medium-priced diagnostic technologies which are likely to become widely spread in Britain. PMID:3128877

Szczepura, A K; Stilwell, J A

1988-01-01

212

Comparison of conventional and organic management conditions on growth performance, carcass characteristics and haematological parameters in Karacabey Merino and Kivircik breeds.  

PubMed

Growth performance, carcass characteristics, post-slaughtering and haematological parameters of Kivircik and Karacabey Merino male lambs in conventional and organic management systems were compared. The animals which were weaned at 7 weeks of age were divided into Kivircik conventional, Kivircik organic (KO), Karacabey Merino conventional and Karacabey Merino organic (MO) groups containing 12 lambs each. Fattening was ended when lambs attained 35 kg of live weight. The time to attain the determined fattening weight was significantly different among the groups, and Merino lambs having higher live weight gain were earlier than Kivircik lambs (p?

Soysal, Deniz; Cibik, Recep; Aydin, Cenk; Ak, ?brahim

2010-12-15

213

Protective effect of herbal and probiotics enriched diet on haematological and immunity status of Oplegnathus fasciatus (Temminck & Schlegel) against Edwardsiella tarda.  

PubMed

This study determines the effect of diet enriched with the herb Baical skullcap Scutellaria baicalensis, and/or probiotics Lactobacillus sakei BK19 in rock bream, Oplegnathus fasciatus (32 ± 3 g) against Edwardsiella tarda. The changes in haematological parameters, innate immune response, and disease resistance were investigated after 1, 3, and 6 weeks. The white blood cell count (WBC: 10(4) mm(-3)), red blood cell count (RBC: 10(6) mm(-3)), and haemoglobin (Hb: g dl(-1)) levels significant increased (P < 0.05) with mixed diet on 3rd and 6th week and probiotics enriched diet on 6th week. The haematocrit (Ht: %) level significantly increased (P < 0.05) when fed with mixed diet on weeks 1-6. Interestingly, in mixed diet group the lymphocytes (LYM), monocytes (MON), and neutrophils (NEU) significantly increased from week 1-6. The eosinophils (EOS) significantly increased in all the treated groups. In the probiotics or mixed diet groups the total protein (TP: g dl(-1)) increased significantly on weeks 3 and 6. The serum lysozyme activity significantly was enhanced in all the treated groups indicating an increase in the innate immunity level. Serum complement, antiprotease activities, reactive oxygen species (ROS) and reactive nitrogen species (RNS) production significantly increased from week 1-6 with mixed diet. The maximum protection against E. tarda was recorded in mixed diet group with a minimum cumulative mortality of 20% and a high relative percent survival (RPS) of 72.84. In the probiotics and herbal diet groups the cumulative mortality was 25% and 35% and RPS was 68.63 and 59.42, respectively. This study indicates that administration of probiotics or mixed diets can effectively minimize the mortality and restore the altered hematological parameters and enhancing the innate immunity in O. fasciatus against E. tarda. PMID:21272648

Harikrishnan, Ramasamy; Kim, Man-Chul; Kim, Ju-Sang; Balasundaram, Chellam; Heo, Moon-Soo

2011-01-25

214

A comparative study of clinical manifestations, haematological and serological responses after experimental infection with Anaplasma phagocytophilum in two Norwegian sheep breeds  

PubMed Central

Background It has been questioned if the old native Norwegian sheep breed, Old Norse Sheep (also called Norwegian Feral Sheep), normally distributed on coastal areas where ticks are abundant, is more protected against tick-borne infections than other Norwegian breeds due to a continuously high selection pressure on pasture. The aim of the present study was to test this hypothesis in an experimental infection study. Methods Five-months-old lambs of two Norwegian sheep breeds, Norwegian White (NW) sheep and Old Norse (ON) sheep, were experimentally infected with a 16S rRNA genetic variant of Anaplasma phagocytophilum (similar to GenBank accession number M73220). The experiment was repeated for two subsequent years, 2008 and 2009, with the use of 16 lambs of each breed annually. Ten lambs of each breed were inoculated intravenously each year with 0.4 ml A. phagocytophilum-infected blood containing approximately 0.5 × 106 infected neutrophils/ml. Six lambs of each breed were used as uninfected controls. Half of the primary inoculated lambs in each breed were re-challenged with the same infectious dose at nine (2008) and twelve (2009) weeks after the first challenge. The clinical, haematological and serological responses to A. phagocytophilum infection were compared in the two sheep breeds. Results The present study indicates a difference in fever response and infection rate between breeds of Norwegian sheep after experimental infection with A. phagocytophilum. Conclusion Although clinical response seems to be less in ON-lambs compared to NW-lambs, further studies including more animals are needed to evaluate if the ON-breed is more protected against tick-borne infections than other Norwegian breeds.

2011-01-01

215

Evaluation of the coulter LH 750 haematology analyzer compared with flow cytometry as the reference method for WBC, platelet and nucleated RBC count.  

PubMed

The Coulter LH 750 is a new haematology analyser with several new features: a count of nucleated red blood cells (NRBCs), automated WBC correction in presence of a flag indicating a cellular interference and a lower incidence of platelet or WBC interference flags when compared with the GEN.S, our current instrument. We had three main goals in our study: evaluating the LH 750 WBC counts when a GEN.S flag suggests a risk of WBC interference, ascertaining whether the platelet counts not flagged by the LH 750 were accurately assessed in samples flagged by the GEN.S and evaluating the NRBC assay provided by the LH 750. Flow cytometry, using CD45 and CD41, respectively for WBC and platelet labelling, was used as a reference method to assess the accuracy of the LH 750 counts. NRBC were identified by double labelling with propidium iodide (PI) and CD45, NRBCs being CD45-/PI+. A significant relationship was found between LH 750 and flow cytometric WBC counts, whether a WBC correction was made by the LH 750 (r = 0.9809, n = 54) or not (r = 0.9901, n = 23). A highly significant relationship was observed for platelets not only in the range from 0 to 450 x 10(9)/l (r = 0.981, n = 108) but also in cases of thrombocytopenia (range: 0-80 x 10(9)/l; r = 0.956, n = 51). In samples with NRBCs, the NRBC percentages given by the LH 750 and by flow cytometry were highly correlated (r = 0.977, n = 60) and WBC counts were accurate. In conclusion, the reduction in flagging by the LH 750, the accuracy of the results, and the availability of a NRBC count, constitute major advantages. PMID:14738430

Igout, J; Fretigny, M; Vasse, M; Callat, M P; Silva, M; Willemont, L; Gelle, M; Lenormand, B

2004-02-01

216

A prospective molecular study of chimaerism in patients with haematological malignancies receiving unrelated cord blood or bone marrow transplants: detection of mixed chimaerism predicts graft failure with or without early autologous reconstitution in cord blood recipients.  

PubMed

We prospectively studied the chimaerism status in the bone marrow (BM) and peripheral blood (PB) of 23 patients receiving umbilical cord (UCB, 14 cases) or BM (nine cases) transplants from unrelated donors by PCR amplification of four individual-specific VNTR genetic loci. Haematological engraftment, with persistent full donor pattern. was observed in 10/14 (72%) patients receiving UCB and in 9/9 (100%) patients transplanted with marrow from an unrelated donor (MUD). In contrast, the remaining four patients converted to an autologous pattern. Three out of these four patients had an early autologous haematological reconstitution reaching a neutrophil level >0.5 x 10(9)/l at days 27, 33 and 37 after transplant, respectively. In all three of these patients, chimaerism analysis demonstrated an early appearance of donor cells (i.e. within 35 d after UCB transplant) showing a transient full donor (one case) or mixed chimaerism condition (two cases). Despite the early autologous haemopoietic reconstitution, one of the three patients died of GVHD at day 60, which was explained by the demonstration of low levels of donor lymphoid cells. In the MUD group all nine patients converted to a persistent full donor pattern with haematological reconstitution, accompanied in two of them by transient mixed chimaerism lasting to days 60 and 270 after transplant. Our data show that monitoring of chimaerism may predict graft failure with or without early autologous haemopoietic reconstitution in patients receiving unrelated UCB transplants. Furthermore, chimaerism analysis may identify, in patients with autologous reconstitution, those at risk of severe GVHD in whom immunosuppressive therapy should not be discontinued. PMID:10192439

Cimino, G; Rapanotti, M C; Elia, L; Iori, A P; Guglielmi, C; Screnci, M; Carmini, D; De Felice, L; Moleti, M L; Mengarelli, A; Mandelli, F; Arcese, W

1999-03-01

217

In vitro activities of amphotericin B and AmBisome against Aspergillus isolates recovered from Italian patients treated for haematological malignancies.  

PubMed

Although there is evidence that liposomal amphotericin B (AmBisome) is non-inferior to amphotericin B (AmB) in terms of in vivo efficacy, in vitro data regarding the activity of AmBisome against clinical isolates of Aspergillus are rare. In this study, the susceptibilities to AmB and AmBisome of 103 Aspergillus complex isolates (48 Aspergillus flavus, 33 Aspergillus fumigatus, 13 Aspergillus terreus and 9 Aspergillus niger) recovered from haematological patients with invasive infection were compared. Minimum inhibitory concentrations (MICs) were determined by the broth microdilution (BMD) method according to the Clinical and Laboratory Standards Institute (CLSI), whilst AmB susceptibility was also determined by Etest. Using a susceptible/resistant MIC cut-off of 1mg/L, all A. fumigatus and A. niger complexes isolates were susceptible to both AmB and AmBisome. In contrast, 38.5% and 30.8% of the A. terreus complex isolates were resistant to AmB and AmBisome, respectively, with good agreement between BMD and Etest methods. With respect to A. flavus complex isolates, 43.7% and 16.7% were resistant by the BMD method to AmBisome and AmB, respectively. For isolates with discrepant results, AmB MICs obtained by Etest were higher than those obtained for AmB by the BMD method and they were closer to those obtained for AmBisome by BMD. Aspergillus flavus AmB MICs ranged from 0.5 mg/L to 2 mg/L by the BMD method and from 1 mg/L to >16 mg/L by the Etest method, and AmBisome MICs ranged from 0.06 mg/L to >16 mg/L by the BMD method. Etest appears to be superior to the CLSI BMD method using AmB in detecting AmB resistance of Aspergillus spp., although the CLSI BMD method might be a suitable procedure if AmBisome is used as the test drug. PMID:22429723

Colozza, Camilla; Posteraro, Brunella; Santilli, Stefania; De Carolis, Elena; Sanguinetti, Maurizio; Girmenia, Corrado

2012-03-17

218

Endocannabinoids and the haematological system  

PubMed Central

Endocannabinoids are blood borne and may also be secreted by the endothelium. Accordingly, there has been interest in the interactions between (endo)cannabinoids and blood cells. There is certainly evidence that (endo)cannabinoids may promote platelet activation, indicating that they may be thrombogenic. Platelets are involved both in the metabolism and release of endocannabinoids, and so it is possible that their circulating levels may be regulated by platelets. This process is altered in disease states such that platelet-derived endocannabinoids contribute towards hypotension in cardiovascular shock. Not only may endocannabinoids regulate platelet function and possibly lead to thrombogenesis, but they may also influence haematopoiesis. Given these emerging roles, the aim of this review is to examine the interactions between cannabinoids and blood.

Randall, M D

2007-01-01

219

Long-term complete haematological and molecular remission after allogeneic bone marrow transplantation in a patient with a stem cell myeloproliferative disorder associated with t(8;13)(p12;q12).  

PubMed

A rare atypical myeloproliferative disorder (aMPD) associated with chromosomal translocations involving the short arm of chromosome 8, region p11-p12 has been described. In most patients, the cytogenetic abnormality is a t(8;13)(p12;q12) that fuses fibroblast growth factor receptor 1, the 8p12 key gene, to FIM/ZNF198 gene. Prognosis is poor with frequent evolution to acute myeloid leukaemia within 1 year of diagnosis. We report a new patient with aMPD with a t(8;13) translocation. Complete haematological, cytogenetic and molecular remission was demonstrated 39 months after allogeneic bone marrow transplantation. This is the first report to demonstrate a molecular remission in this disorder. PMID:12694254

Suzan, Florence; Guasch, Géraldine; Terre, Christine; Garcia, Isabel; Bastie, Jean-Noël; Maarek, Odile; Ribaud, Patricia; Gluckman, Eliane; Daniel, Marie-Thérčse; Pébusque, Marie-Josčphe; Castaigne, Sylvie

2003-04-01

220

Cardiovascular, haematological and neurological effects of the venom of the Papua New Guinean small-eyed snake (Micropechis ikaheka) and their neutralisation with CSL polyvalent and black snake antivenoms.  

PubMed

Cardiovascular and haematological effects of venom of the small-eyed Snake (Micropechis ikaheka) were examined in ventilated anaesthetised piglets. Neurotoxic effects were examined in chick biventer cervicis nerve-muscle preparations. Immunoreactivity of venom was tested against the monovalent antivenom components in a CSL Ltd Venom Detection Kit. Neutralisation was tested in vivo and in vitro with CSL Ltd polyvalent snake and Black Snake (Pseudechis australis) antivenoms. Venom in 0.1% bovine serum albumin in saline was infused into piglets in doses 1-2000 microg/kg. Pulmonary hypertension (P= 0.0007) and depression of cardiac output (P= 0.002) were observed up to 3 h after 150-160 microg/kg. The concentration of plasma free-haemoglobin increased more than 50-fold, indicating haemolysis. Neither coagulopathy nor thrombocytopenia occurred. Creatine phosphokinase and serum potassium levels did not increase suggesting absence of acute rhabdomyolysis. The venom caused post-synaptic neurotoxicty. Immunoreactivity of venom with Black Snake antivenom was observed at very high venom concentrations. Cardiovascular effects were absent and haemolysis was less after venom was pre-incubated at 37 degrees C for 30 min with polyvalent antivenom. Neutralisation by Black Snake antivenom was less effective. The neurotoxicity was neutralised by polyvalent or Black Snake antivenoms. Human envenomation may be treated with CSL Ltd polyvalent snake antivenom. PMID:14602120

Tibballs, J; Kuruppu, S; Hodgson, W C; Carroll, T; Hawdon, G; Sourial, M; Baker, T; Winkel, K

2003-11-01

221

Evaluation of growth performance, serum biochemistry and haematological parameters on broiler birds fed with raw and processed samples of Entada scandens, Canavalia gladiata and Canavalia ensiformis seed meal as an alternative protein source.  

PubMed

The experiment was carried out to investigate the inclusion of underutilised legumes, Entada scandens, Canavalia gladiata and Canavalia ensiformis, seed meal in soybean-based diet in broilers. The utilisation of these wild legumes is limited by the presence of antinutrient compounds. Processing methods like soaking followed by autoclaving in sodium bicarbonate solution in E. scandens and C. gladiata and soaking followed by autoclaving in ash solution in C. ensiformis were adopted. The proximate composition of raw and processed samples of E. scandens, C. gladiata and C. ensiformis were determined. The protein content was enhanced in processed sample of E. scandens (46 %) and C. ensiformis (16 %). This processing method had reduced the maximum number of antinutrients such as tannins (10-100 %), trypsin inhibitor activity (99 %), chymotrypsin inhibitor activity (72-100 %), canavanine (60-62 %), amylase inhibitor activity (73-100 %), saponins (78-92 %), phytic acid (19-40 %) and lectins. Hence, the raw samples at 15 % and processed samples at 15 and 30 % were replaced with soybean protein in commercial broiler diet respectively. Birds fed with 30 % processed samples of E. scandens, C. gladiata and C. ensiformis showed significantly similar results of growth performance, carcass characteristics, organ weight, haematological parameters and serum biochemical parameters (cholesterol, protein, bilirubin, albumin, globulin and liver and kidney function parameters) without any adverse effects after 42 days of supplementation. The proper utilisation of these underutilised legumes may act as an alternative protein ingredient in poultry diets. PMID:23076820

Sasipriya, Gopalakrishnan; Siddhuraju, Perumal

2012-10-18

222

Feeding a diet contaminated with ochratoxin A for chickens at the maximum level recommended by the EU for poultry feeds (0.1 mg/kg). 1. Effects on growth and slaughter performance, haematological and serum traits.  

PubMed

The European Commission Recommendation 2006/576/EC, suggests that the maximum level of Ochratoxin A (OTA) in poultry feeds should be set at 0.1 mg OTA/kg. Thirty-six one-day-old male Hubburd broiler chickens were divided into two groups, a Control (basal diet) and an Ochratoxin A (basal diet + 0.1 mg OTA/kg) group. The growth and slaughter performance traits were recorded. The liver, spleen, bursa of Fabricius and thymus weights were measured. The erythrocyte and leukocyte numbers were assayed in blood samples, and the heterophils to lymphocytes (H/L) ratio was determined. Alpha-1-acid glycoprotein (AGP), lysozyme, the total protein and the electrophoretic pattern were evaluated in serum samples. Liver enzymes (alanino aminotransferase, ALT and aspartate aminotransferase, AST) and kidney function parameters (uric acid and creatinine) were quantified. The results revealed that feeding a 0.1 mg OTA/kg contaminated diet to chicks caused a decrease in the absolute thymus weight (p < 0.05) and a lower total protein (p < 0.01), albumin (p < 0.01), alpha (p < 0.05), beta (p = 0.001) and gamma (p = 0.001) globulins serum concentration in the Ochratoxin A group. Moreover, the albumin-to-globulin (A/G) ratio of the OTA-treated animals resulted to be higher (p < 0.05). Feeding broiler chickens, a diet contaminated with the maximum level admitted by the European Commission Recommendation (0.1 mg OTA/kg), did not affect the animal performance, slaughter traits, organ weights, haematological parameters, liver enzyme or renal function parameters concentrations but had an overall immunosuppressant effect, with reduction in the thymus weight and of the total serum protein, albumin, alpha, beta and gamma globulins concentration. PMID:23639013

Pozzo, L; Salamano, G; Mellia, E; Gennero, M S; Doglione, L; Cavallarin, L; Tarantola, M; Forneris, G; Schiavone, A

2013-05-01

223

Iron overload: accuracy of in-phase and out-of-phase MRI as a quick method to evaluate liver iron load in haematological malignancies and chronic liver disease  

PubMed Central

Objectives The purpose of this prospective study was to evaluate the accuracy of in-phase and out-of-phase imaging to assess hepatic iron concentration in patients with haematological malignancies and chronic liver disease. Methods MRI-based hepatic iron concentration (M-HIC, ?mol g–1) was used as a reference standard. 42 patients suspected of having iron overload and 12 control subjects underwent 1.5 T in- and out-of-phase and M-HIC liver imaging. Two methods, semi-quantitative visual grading made by two independent readers and quantitative relative signal intensity (rSI) grading from the signal intensity differences of in-phase and out-of-phase images, were used. Statistical analyses were performed using the Spearman and Kruskal–Wallis tests, receiver operator curves and ? coefficients. Results The correlations between M-HIC and visual gradings of Reader 1 (r=0.9534, p<0.0001) and Reader 2 (r=0.9456, p<0.0001) were higher than the correlations of the rSI method (r=0.7719, p<0.0001). There was excellent agreement between the readers (weighted ?=0.9619). Both visual grading and rSI were similar in detecting liver iron overload: rSI had 84.85% sensitivity and 100% specificity; visual grading had 85% sensitivity and 100% specificity. The differences between the grades of visual grading were significant (p<0.0001) and the method was able to distinguish different degrees of iron overload at the threshold of 151 ?mol g–1 with 100% positive predictive value and negative predictive value. Conclusion Detection and grading of liver iron can be performed reliably with in-phase and out-of-phase imaging. Liver fat is a potential pitfall, which limits the use of rSI.

Virtanen, J M; Pudas, T K; Ratilainen, J A; Saunavaara, J P; Komu, M E; Parkkola, R K

2012-01-01

224

Communicating with haematology patients: a reflective account.  

PubMed

Nurses are required to keep their knowledge and skills up to date to ensure their practice is evidence-based. Reflection in and on practice can be used to ensure care provision is evidence-based, to increase self-awareness and to promote positive change. Timely communication of high-quality information tailored to patients' needs is required. When a breakdown in communication occurs in practice, it can have a negative effect on patients and the professionals providing their care. Using an example from my practice experience, this article aims to highlight the importance of communication, reflection and knowledge in nursing practice. PMID:24063485

Savage, Melanie

2013-09-25

225

Fluoride-induced haematological changes in rabbits  

Microsoft Academic Search

Although the effect of fluoride ingestion on the blood cell profile in experimental animals was studied earlier by HIRA0 (1972) who pointed out changes in hemoglobin content, population of erythrocytes, reticulocytes, platelets, leucocyte, and hematocrit, the information is inadequate to provide a scientific rationale for the wide spectrum of deviations observed both in experimental and human fluorosis. According to HIRA0

A. K. Susheela; S. K. Jain

1983-01-01

226

Some haematological data on pregnant Gurkha women.  

PubMed

In a retrospective survey of 285 pregnancies in Gurkha women living in the New Territories of Hong Kong, the mean values of Haemoglobin, Mean Cell Volume and Mean Cell Haemoglobin Concentration were examined with the aim of establishing basic data. PMID:6716356

Jefferson, T O; Reidy, A J; Kennedy, P M

1984-02-01

227

British Committee for Standards in Haematology  

Microsoft Academic Search

Summary Unselected coagulation testing is widely practiced in the process of assessing bleeding risk prior to surgery. This may delay surgery inappropriately and cause unnecessary concern in patients who are found to have 'abnormal' tests. In addition it is associated with a significant cost. This systematic review was performed to determine whether patient bleeding history and unselected coagulation testing predict

Y. L. Chee; J. C. Crawford; H. G. Watson; M. Greaves

228

Improving TCR Gene Therapy for Treatment of Haematological Malignancies  

PubMed Central

Adoptive immunotherapy using TCR gene modified T cells may allow separation of beneficial Graft versus tumour responses from harmful GvHD. Improvements to this include methods to generate high avidity or high affinity TCR, improvements in vector design and reduction in mispairing. Following adoptive transfer, TCR transduced T cells must be able to survive and persist in vivo to give most effective antitumour responses. Central memory or naive T cells have both been shown to be more effective than effector cells at expanding and persisting in vivo. Lymphodepletion may enhance persistence of transferred T cell populations. TCR gene transfer can be used to redirect CD4 helper T cells, and these could be used in combination with CD8+ tumour specific T cells to provide help for the antitumour response. Antigen specific T regulatory T cells can also be generated by TCR gene transfer and could be used to suppress unwanted alloresponses.

Nicholson, Emma; Ghorashian, Sara; Stauss, Hans

2012-01-01

229

Haematological findings in pregnant, postparturient and nursing mares  

Microsoft Academic Search

Venous blood samples were collected from 16 Thoroughbred and Quarter Horse mares during late pregnancy (approximately one month before parturition), approximately 6 h after parturition, one month after parturition and 4 months after parturition when the foals were weaned. There were no significant differences over time for haematocrit, mean cell volume, mean cell haemoglobin concentration, platelet count or mean platelet

J. W. Harvey; R. L. Asquith; M. G. Patel; J. Kivipelto; C. L. Chen; E. A. Ott

1994-01-01

230

Effects of electroplating effluent on haematological parameters of Oreochromis mossambicus  

NASA Astrophysics Data System (ADS)

Fresh water fish, Oreochromis mossambicus was exposed to Electroplating effluent with a view to check the hematological changes as a function of time and effluent concentration. Increase in time and concentration always produced a declining value of RBC, Hb, Ht and WBC. This level is statistically significant in most of the test concentrations and in the experimental duration. The calculated MCV, MCH and MCHC also exhibit the same trend in all the selected sub lethal concentrations of the effluent, which is also found to be time and dose dependent.

Navaraj, P. S.; Kumaraguru, A. K.

2003-05-01

231

HAEMATOLOGIC PARAMETERS ON VARIOUS SPECIES OF STRIGIFORMES AND FALCONIFORMES  

Microsoft Academic Search

Normal mean values for packed cell volume, total erythrocyte count, total leukocyte count, total protein and mean corpuscular volume were obtained from 37 species of Strigiforines and Falconiformes representing 207 individuals.

ELIZABETH E. SMITH

1978-01-01

232

Zearalenone induces modifications of haematological and biochemical parameters in rats.  

PubMed

Zearalenone produced by the fungus Fusarium roseum causes important perturbations in the gestation cycle of the rat with hormonal disorders and infertility. In order to find out other eventual toxic effects, female rats were given intraperitoneally (i.p.) (1.5, 3 and 5 mg/kg) zearalenone in sterile olive oil. Forty-eight hours later, some blood parameters changed (hematocrit, MCV, the number of platelets and WBC) as well as some biochemical markers such as aminotransferases (ALT, AST), alkaline phosphatase (ALP), serum creatinine, bilirubin, indicating liver toxicity, and likely impairment of blood coagulation process. PMID:8783448

Maaroufi, K; Chekir, L; Creppy, E E; Ellouz, F; Bacha, H

1996-05-01

233

Haematological and biochemical observations during a 750 mile relay  

Microsoft Academic Search

During a marathon relay from John O'Groats to Lands End (JOGLE) various blood and urine parameters were measured in the runners. Samples of blood and urine were taken from each runner immediately prior to the event and on two occasions during it. Subsequently a three point plot was made for each parameter measured. No special diet was taken. Food and

C R Nyman

1985-01-01

234

Predictors of Parenting Stress in Patients with Haematological Cancer  

Microsoft Academic Search

This study was designed to identify potential determinants of parenting stress in parents with leukemia or lymphoma and with children younger than age 18 years. Participants recruited at a comprehensive cancer center completed self-report measures of parenting stress, attachment style, parenting alliance, depression, and anxiety. Depression was positively correlated with parenting stress. Anxious and avoidant attachment styles, but not the

Cheryl Fernandes; Robert Muller; Gary Rodin

2012-01-01

235

Malaria during pregnancy and foetal haematological status in Blantyre, Malawi  

Microsoft Academic Search

BACKGROUND: Although maternal anaemia often stems from malaria infection during pregnancy, its effects on foetal haemoglobin levels are not straightforward. Lower-than-expected cord haemoglobin values in malarious versus non-malarious regions were noted by one review, which hypothesized they resulted from foetal immune activation to maternal malaria. This study addressed this idea by examining cord haemoglobin levels in relation to maternal malaria,

Elizabeth T Abrams; Jesse J Kwiek; Victor Mwapasa; Deborah D Kamwendo; Eyob Tadesse; Valentino M Lema; Malcolm E Molyneux; Stephen J Rogerson; Steven R Meshnick

2005-01-01

236

Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies  

Microsoft Academic Search

The rapid diagnosis of an infectious cause in the course of fever of unknown origin plays a pivotal role in the correct management of neutropenic patients. In this study, blood samples from febrile oncohaematological patients were tested using a novel commercial real-time PCR assay (LightCycler SeptiFast; Roche Molecular Systems) and blood culture (BacT\\/Alert 3D; bioMerieux). Twenty-one (20.4%) and 34 (33%)

Nicasio Mancini; Daniela Clerici; Roberta Diotti; Mario Perotti; Nadia Ghidoli; Donata De Marco; Beatrice Pizzorno; Thomas Emrich; Roberto Burioni; Fabio Ciceri; Massimo Clementi

2008-01-01

237

Progress in fighting systemic fungal infections in haematological neoplasia.  

PubMed

Considering the limited data available, there is clearly a need for thorough, well-designed clinical research on the epidemiology, diagnosis, treatment and prevention of invasive fungal infection in patients who are treated for cancer. Our knowledge has increased, but the information obtained so far is patchy and not generally applicable, as it is influenced by local problems and circumstances. New diagnostic tools have become available, but they are still insufficient in many cases. Until the value of the presently available chemoprophylaxis has been established beyond doubt, the strategy should be one of wait-and-see for patients with a low or moderate risk of developing infection. In bone marrow transplant recipients fluconazole has shown favourable results in eliminating yeast infections, but in patients at high risk of mould infections early initiation of intravenous treatment with amphotericin B at a therapeutic dose remains the best approach. The question of the optimal time point to start empirical antifungal treatment remains and has even been extended by the dispute about what antifungal drugs should be used for this purpose. Amphotericin B is still the drug of choice for the treatment of disseminated fungal infection, but its lipid formulations seem to offer a safer, though far more expensive, alternative. Head-to-head comparisons between the different formulations are required before a final conclusion on their respective efficacies and toxicities can be drawn, and it is questionable whether a higher dose will produce better results. Fluconazole appears very useful against the majority of Candida infections, whereas itraconazole is effective against both yeast and moulds, providing that adequate resorption can be ensured. The results of the first clinical trial of voriconazole in pulmonary aspergillosis have proved very promising. PMID:9458534

De Pauw, B E; Meis, J F

1998-01-01

238

Predictors of parenting stress in patients with haematological cancer.  

PubMed

This study was designed to identify potential determinants of parenting stress in parents with leukemia or lymphoma and with children younger than age 18 years. Participants recruited at a comprehensive cancer center completed self-report measures of parenting stress, attachment style, parenting alliance, depression, and anxiety. Depression was positively correlated with parenting stress. Anxious and avoidant attachment styles, but not the degree of physical distress or the quality of the parenting alliance, significantly predicted parenting stress. These findings underscore the relationship of parenting stress to psychological well-being and the contribution of individual characteristics in parents to this stress. PMID:22269077

Fernandes, Cheryl; Muller, Robert; Rodin, Gary

2012-01-01

239

Platelet transfusion therapy in thrombocytopaenia of haematologic malignancies.  

PubMed

Thirty thrombocytopaenic patients of acute leukaemias and myelodysplastic syndrome were transfused platelets collected from ABO-matched donors using Haemonetics V30 and V50 blood processors. Twenty-seven patients had septicaemia and/or splenomegaly; 2 patients had disseminated intravascular coagulation (DIC). Pre-transfusion platelet count was 11.0 +/- 4.0 X 10(9)/L. The mean corrected count increments (CCI) 1 hour and 18 hours post-transfusion were 13.02 X 10(9)/L and 3.88 X 10(9)/L respectively, in the absence of DIC. Active bleeding stopped when platelet count was above 15.0 X 10(9)/L. There was no difference between the platelet yield from two blood processors. PMID:2768161

Charak, B S; Rao, K; Parikh, P M; Rawat, R S; Nair, C N; Advani, S H

1989-03-01

240

Not so benign haematology: anaemia of the elderly  

PubMed Central

Summary Developed countries, such as the United Kingdom, are experiencing a change in demographics resulting in the largest proportion of adults over 65 years of age that our health systems have ever experienced. As such, haematologists must be prepared to evaluate and treat anaemia in a more complicated patient population, but sufficient evidence-based guidelines are lacking. Critical next steps that must be taken to ensure the best care of this population include the determination of appropriate haemoglobin concentrations for older adults in light of age, gender, race, and comorbidities; the development of interventional trials that address physical performance outcomes in addition to haemoglobin targets; and translational studies which address the molecular pathogenesis of anaemia in older adults with the most advanced scientific approaches.

Merchant, Akil A.; Roy, Cindy N.

2011-01-01

241

Splenectomy for non-haematological metastatic malignant disease  

Microsoft Academic Search

Purpose  Multiple cases and small series of patients who have undergone splenectomy for metastatic malignant disease have been reported.\\u000a This study examines the outcome of patients with metastatic malignant disease to the spleen treated by splenectomy at a tertiary\\u000a oncology centre and a review of cases published in the last 10 years.\\u000a \\u000a \\u000a \\u000a \\u000a Methods  The hospital histopathology database was searched over a 25-year period

Piers A. C. Gatenby; Satvinder S. Mudan; Andrew C. Wotherspoon

2011-01-01

242

Disseminated intravascular coagulation in obstetric disorders and its acute haematological management.  

PubMed

As activation of the coagulation pathway is a physiological response to injury, the development of disseminated intravascular coagulation (DIC) is a warning signal to the clinician that the primary pathological disease state is decompensating. In pregnancy, DIC can occur in several settings, which include emergencies such as placental abruption and amniotic fluid embolism as well as complications such as pre-eclampsia. Whilst the acuteness of the event and the proportionality in the coagulant and fibrinolytic responses may vary between these different conditions, a common theme for pregnancy-associated DIC is the pivotal role played by the placenta. Removal of the placenta is the linchpin to treatment in most cases but appropriate blood product support is also key to management. This is necessary because DIC itself can have pathological consequences that translate clinically into a worse prognosis for affected patients. This article will describe how pregnancy-associated DIC can be diagnosed promptly and how treatment should be managed strategically. It also discusses the latest developments in our understanding of haemostatic mechanisms within the placenta and how these may have relevance to new diagnostic approaches as well as novel therapeutic modalities. PMID:19442424

Thachil, Jecko; Toh, Cheng-Hock

2009-05-12

243

Perinatal and reproductive factors: a report on haematological malignancies from the UKCCS  

Microsoft Academic Search

The United Kingdom Childhood Cancer Study was designed to examine the potential aetiological role of a range of perinatal and reproductive factors. Our use of clinical records permitted a more exact characterisation of reproductive events than is possible in investigations that rely on self-reporting; and the increased specificity with which antecedent events were measured produced more precise risk estimates, albeit

E. Roman; J. Simpson; P. Ansell; T. Lightfoot; C. Mitchell; T. O. B. Eden

2005-01-01

244

Effects of fasting on haematology and clinical chemistry values in the rat and dog  

Microsoft Academic Search

Many regulatory toxicity guidelines and the recommendation of AACC-DACC\\/ASVCP joint task force of the USA on clinical pathology testing require overnight fasting for rats and non-rodents before blood sampling. However, the reason why animals must be fasted before blood sampling is unclear in toxicology studies.

T. Matsuzawa; M. Sakazume

1994-01-01

245

Red blood cell function and haematology in two tropical freshwater fishes from Australia  

Microsoft Academic Search

Salmon catfish and tarpon occur in habitats that periodically become deficient in oxygen resulting in high mortalities of other fish species. The water-breathing catfish, Arius leptaspis, and the facultative air-breathing tarpon, Megalops cyprinoides, both have high haemoglobin and haematocrit, and the oxygen carrying capacity in the air-breather is exceptionally high (15.6±1.2 vol%). Iso-pH oxygen equilibria of the red blood cells

R. M. G. Wells; J. Baldwin; R. S. Seymour; K. Christian; T. Brittain

2005-01-01

246

Red blood cell function and haematology in two tropical freshwater fishes from Australia.  

PubMed

Salmon catfish and tarpon occur in habitats that periodically become deficient in oxygen resulting in high mortalities of other fish species. The water-breathing catfish, Arius leptaspis, and the facultative air-breathing tarpon, Megalops cyprinoides, both have high haemoglobin and haematocrit, and the oxygen carrying capacity in the air-breather is exceptionally high (15.6+/-1.2 vol%). Iso-pH oxygen equilibria of the red blood cells at 25 degrees C revealed high affinity (P(50)=9 mmHg, pH 7.4) and co-operativity (n(50)>2.2, pH 7.4) in the catfish, and contrasted with low affinity (P(50)=32 mmHg, pH 7.4) and co-operativity (n(50) approximately 1) in the air-breathing tarpon. Oxygen binding was further distinguished by relative pH insensitivity (Bohr factor, Ř=Deltalog P(50)/Deltalog pH=-0.22) in the catfish, compared with a significant Bohr effect in the tarpon (Ř=-0.96). The potential for modulation of haemoglobin-oxygen affinity was indicated by a high ratio of GTP to ATP in the erythrocytes of the catfish, whereas regulation in the tarpon appeared due to ATP alone. Differences in blood respiratory functions between the two species are likely to reflect reduced opportunity for activity under extreme hypoxia in the catfish. PMID:15893948

Wells, R M G; Baldwin, J; Seymour, R S; Christian, K; Brittain, T

2005-05-01

247

Posaconazole prophylaxis - impact on incidence of invasive fungal disease and antifungal treatment in haematological patients.  

PubMed

Since two large-scale, randomised studies on posaconazole prophylaxis have demonstrated a clear benefit for patients at high risk for contracting invasive fungal disease (IFD), posaconazole prophylaxis has been adopted as standard of care for this patient collective. Several years on from implementation at our institution, we wanted to evaluate its impact on the incidence and use of empirical antifungal therapy in a real-life setting. We analysed retrospectively incidence and severity of IFD in high-risk patients with prophylaxis, using a historical cohort as comparator. A total of 200 patients had either received the extended spectrum triazole posaconazole in prophylactic dosage of 200 mg tid or empirical antifungal therapy. Disease events were analysed by application of the revised EORTC/MSG definitions for IFD. Before posaconazole prophylaxis, we recorded 57/100 cases of IFD which was reduced to 28/100 with prophylaxis. The empirical use of antifungal drugs was reduced to 41% from 91% in the non-prophylaxis cohort. Furthermore, we observed a shift in the categorisation of IFD according to EORTC/MSG criteria. Our data suggest that posaconazole was effective in reducing the rate and probability of invasive fungal disease in high-risk patients. PMID:23710592

Peterson, Lisa; Ostermann, Julia; Rieger, Heidi; Ostermann, Helmut; Rieger, Christina Theresa

2013-05-26

248

89 Sr radionuclide therapy: Dosimetry and haematological toxicity in two patients with metastasising prostatic carcinoma  

Microsoft Academic Search

We present dosimetry for spinal metastases and red bone marrow in two patients who received 89Sr therapy for disseminated prostatic carcinoma. Absorbed dose to metastases was estimated by combining 85Sr gamma camera studies with computed tomographic measurements of bone mass, and doses of 20 cGy\\/MBq and 24 cGy\\/MBq were found for vertebral metastases that uniformly involved the bodies of L3

G. M. Blake; M. A. Zivanovic; A. J. McEwan; V. B. Batty; D. M. Ackery

1987-01-01

249

Haematological changes following systemic injection of purified carrageenans (kappa, lambda and iota).  

PubMed Central

Kappa, lambda and iota carrageenans were administered i.p. (125 mg/kg) to groups of Sprague-Dawley rats. Each carrageenan (but especially kappa and lambda) caused thrombocytopenia and red-cell damage, particularly burring, within 2 days. This was followed by rebound thrombocytosis and persistent anaemia, accompanied by a reticulocytosis. A 2-fold increase in fibrinogen was observed at 24 or 48 h. All groups showed a leucopenia at 1 h, then a progressive leucocytosis, maximal at 48 h (kappa and lambda) or Day 7 (iota). Between 1 and 24 h there was a significant lymphopenia, followed by lymphocytosis (kappa and lambda) including Turk cells and pronounced neutrophilia in all groups. Monocytosis occurred in response to each carrageenan on Days 2-4 (kappa) or Day 7 (lambda and iota). Injection of kappa carrageenan was characterized by the presence (up to Day 7) of carrageenan-positive material, in the form of floccules, within the peripheral blood, and by Day 7 the appearance of histiocyte-type macrophages which exhibited haemo-phagocytosis. In the lambda group, carrageenan-positive granules were observed in the cytoplasm of mononuclear cells throughout the experimental period. No intracellular carrageenan was demonstrable in peripheral blood in the iota group or within neutrophils of any of the injected animals. Overall marrow cellularity was not altered by carrageenan, but small numbers of kappa- and lambda-containing macrophages were identified. Splenomegaly was consistently observed and in histological section carrageenan-positive macrophages were detected in the red pulp, particularly in the lambda group. Images Fig. 3 Fig. 5 Fig. 6 Fig. 7 Fig. 8 Fig. 9 Fig. 10

Davidson, R. J.; Simpson, J. G.; Whiting, P. H.; Milton, J. I.; Thomson, A. W.

1981-01-01

250

Haematological Parameters of Fayoumi, Assil and Local Chickens Reared in Sylhet Region in Bangladesh  

Microsoft Academic Search

2 Abstract: An experiment was conducted to study the hematological parameters in Fayoumi, Assil and Local Chickens of different ages reared in Sylhet region. 250 chickens of three breeds (100 Fayoumi, 50 Assil and 100 Local) were tested at different ages (1, 3, 6, 9 and 12 months) to observe the hematological parameters: i) Total erythrocyte Count (TEC) ii) Packed

2004-01-01

251

Effect of superoxide dismutase on haematologic parameters and urinary changes after burn injury in rats  

Microsoft Academic Search

This study was designed to examine the possible benefits that superoxide dismutase (SOD), a free radical scavenger, could induce upon some of the hematologic and urinary alterations occurring during the burn shock syndrome. The experiment was conducted by comparing two groups of rats treated with SOD and saline solution respectively, immediately after inducing a scald burn injury. Statistically significant differences

T. Lozano; F. Guemes; F. X. Santos; J. M. Obispo

1993-01-01

252

Clinical, haematological and biochemical findings in milk-fed calves with chronic indigestion.  

PubMed

The principal clinical signs in 59 milk-fed calves with chronic indigestion were general malaise and depression, poor appetite, poor body condition, dehydration, a dull and scaly hair coat, alopecia and clay-like faeces. All the calves had metabolic acidosis, which was associated with an inability to stand up in more than half of them. There were significant differences in the severity of acidosis between the calves that could stand and those that could not. Other signs in some of the calves were dehydration, leucocytosis, and increased activities of liver enzymes. PMID:10515617

Stocker, H; Lutz, H; Rüsch, P

1999-09-11

253

Excluding medical and haematological conditions as a cause of bruising in suspected non-accidental injury  

Microsoft Academic Search

A mistaken diagnosis of child abuse can occur in a number of medical conditions, many of which can be readily diagnosed by experienced paediatricians. Bleeding disorders offer a greater challenge, especially when court proceedings may demand their exclusion. Some of these disorders are rare but more prevalent in areas which have a high incidence of consanguinity. We advocate two stages

A M B Minford; E M Richards

2010-01-01

254

Clinico-haematology and pathology of caprine mycoplasmal pneumonia in rain fed tropics of West Bengal  

Microsoft Academic Search

A total of 4500 suspected mycoplasma infected swabs samples were cultivated bacteriologically both in pleuropneumonia like organism (PPLO) broth and agar of which 310 were found positive for mycoplasma infection. Biochemical characterisation and growth inhibition test with known mycoplasmal antisera revealed involvement of three species of mycoplasma namely Mycoplasma agalactiae (Ma, 102), Mycoplasma mycoides subsp. capri (Mmc, 124) and M.

D Mondal; A. K Pramanik; D. K Basak

2004-01-01

255

Cobalamin deficiency resulting in a rare haematological disorder: a case report  

PubMed Central

Introduction We present the case of a patient with a cobalamin deficiency resulting in pancytopaenia, emphasizing the importance to define, diagnose and treat cobalamin deficiency. Case presentation A 52-year-old man from the Democratic Republic of Congo presented to the emergency department with shortness of breath and a sore tongue. Physical examination was unremarkable. His haemoglobin was low and the peripheral blood smear revealed pancytopaenia with a thrombotic microangiopathy. The findings were low cobalamin and folate levels, and high homocysteine and methylmalonate levels. Pernicious anaemia with chronic atrophic gastritis was confirmed by gastric biopsy and positive antiparietal cell and anti-intrinsic factor antibodies. Cobalamin with added folate was given. Six months later, the patient was asymptomatic. Conclusion Cobalamin deficiency should always be ruled out in a patient with pancytopaenia. Our case report highlights a life-threatening cobalamin deficiency completely reversible after treatment.

2009-01-01

256

Clinical, haematological and biochemical studies of babesiosis in native goats in Mosul  

Microsoft Academic Search

The study included examination of 175 native goats, 27 were infected with Babesia ovis , B. motasi , B. foliata and B. taylori , (recorded in Mosul for the first time) and 25 were clinically normal and served as control. Results indicated that the percentage of the infection with Babesiosis was 15.42% and the percentage of parasitemia ranged between 3.5-10.4%

E. G. Sulaiman; S. H. Arslan; Q. T. Al-Obaidi; E. Daham

257

Gastric ulceration in Persian Arab horses in Iran: frequency, haematology and biochemistry  

Microsoft Academic Search

Summary Gastric endoscopy was performed in the 24 Persian Arab horses from several race training in Tehran and Tabriz for detection of gastric ulcer. Gastric ulcer was evident in 14 Persian Arab horses (58.3%). Ten out of 14 ulcers were in nonglandular region of the stomach. The horses with the history of long term treatment with non-steroidal anti-inflammatory drugs (NSAIDs)

Mokhber Dezfouli; A. Hassanpour; M. R. Mokhber Dezfouli

258

Disseminated infection by Scedosporium Prolificans : an emerging fatality among haematology patients. Case report and review  

Microsoft Academic Search

We describe a case of proven disseminated infection by Scedosporium prolificans in a profoundly neutropenic patient. The patient presented with a fever unresponsive to broad-spectrum antibiotics, endophthalmitis,\\u000a respiratory failure and a renal abscess. The organism was isolated from bronchoalveolar lavage fluid and from pus obtained\\u000a through a sterile puncture. Review of the English-language literature identified 28 additional cases; these occurred

J. Maertens; K. Lagrou; H. Deweerdt; I. Surmont; G. E. G. Verhoef; J. Verhaegen; M. A. Boogaerts

2000-01-01

259

Viral purging of haematological autografts: should we sneeze on the graft?  

Microsoft Academic Search

High-dose cytotoxic chemotherapy followed by autologous haematopoietic stem cell transplantation (ASCT) is extensively used for the treatment of many haematopoietic, as well as several epithelial cancers. Disease relapse may be the result of tumour contamination within autograft as evidenced by gene marking studies. The multiple purging strategies that have been described to date have not proven effective in most ASCT

C M Thirukkumaran; J A Russell; D A Stewart; D G Morris

2007-01-01

260

The ability of biochemical and haematological tests to predict recovery in periparturient recumbent cows  

Microsoft Academic Search

Blood samples from 433 periparturient recumbent cows submitted by veterinary practitioners to Ruakura Animal Health Laboratory during 1983 and 1984 were analysed and results related to whether cows recovered, died or were euthanased. Generally cows were sampled only once and the time varied from 15 minutes to 20 days after becoming recumbent.During 1983 serum calcium, magnesium, phosphorus, creatine phosphokinase (CK),

R. G. Clark; H. V. Henderson; G. K. Hoggard; R. S. Ellison; B. J. Young

1987-01-01

261

Acute megakaryoblastic leukaemia: a clinico-haematological profile of five cases.  

PubMed

Herein we are presenting the clinical, morphological and cytochemical characteristics of five cases of acute megakaryoblastic leukaemia (AML-M7) seen by us over a period of five years (Jan 1996-Dec 2000). Morphological assessment revealed marked polymorphism of blast cells and platelets both in the peripheral blood and bone marrow smears in all cases. Size of the blast cells ranged from very small to very large multinucleated cells, with variable chromatin pattern and number of nucleoli. More differentiated megakaryocytic cells showing cytoplasmic blebs, protrusions and platelet budding with bizarre platelet morphology were characteristic features suggesting the diagnosis. Cytochemical stains like myeloperoxidase, sudan black and PAS were positive in 5-15% of blast cell. Coagulation studies revealed a normal coagulation profile, whereas platelet studies showed marked impairment in aggregation of platelets with ADP and adrenalin with a normal PF-3 availability. PMID:16295495

Shukla, Jyoti; Rai, Sunita; Singh, V P

2004-04-01

262

Variation of 3?-to-2? ratio from 18F in haematological components measured using the GAMMASPHERE  

NASA Astrophysics Data System (ADS)

The potential of incorporating three-quanta annihilation into positron emission tomography (PET) to detect local tissue chemistry was investigated using the GAMMASPHERE facility, which features a spherical array of 110 Compton-suppressed high-purity germanium (HPGe) detectors. Fluoro-2-deoxyglucose (FDG) containing F18 was introduced into 11 samples of haemolysed blood, serum, cell concentrate and whole blood, some of which had been either oxygenated or deoxygenated. The relative three-quanta yield was estimated from the reduced counts in the full-energy photopeak at 511 keV. Compton-suppression produced larger effects in the calculations than time-gating, suggesting its importance for this method of estimation. The relative three-quanta yields were found to vary as much as 11% between the samples. This level of sensitivity to different biological samples commends three-quanta annihilation for molecular imaging.

Chin, M. P. W.; Seweryniak, D.; Alkhorayef, M.; Spyrou, N. M.

2009-06-01

263

Clinical presentation, haematological indices and management of children with severe and uncomplicated malaria in Douala, Cameroon.  

PubMed

This study carried out from January to June 2007, was undertaken to describe the clinical presentation of childhood malaria in Douala, a meso-endemic area as far as malaria transmission is concerned. One hundred and seventy eight children were enrolled after informed consent of their parents. The sample characteristics were recorded and clinical as well as preliminary laboratory investigations were performed. Thirty eight children coming for vaccination and counselling was targeted to serve as control. According to the results obtained, cerebral malaria (CM) seems to be associated with young age, whilst Malaria anaemia (MA) was predominant among older children. Hyperpyrexia and hyperparasitaemia were high among CM patients and 11.1% of them died, however, no neurological squeal was noticed immediately after discharge on those who survived. Haemoglobin and glycaemia were low on MA and CM patients; these groups had low percentage in bed nets utilization as well. These results suggest that the clinical presentation of the disease differ with the geographic location and malaria disease features varies according to the severity. Such studies could contribute to the management of the disease. PMID:19137849

Mfonkeu, J B Pankoui; Gouado, I; Kuate, H Fotso; Zambou, O; Grau, G; Combes, V; Zollo, P H Amvam

2008-10-15

264

Some Haematological and Biochemical Investigations on Duck Virus Hepatitis following Administration of Glycyrrhizin  

PubMed Central

The present study aimed to investigate the protective effect of glycyrrhizin (locally isolated and purified from licorice root) against duck hepatitis virus through the assessment of some hematological and biochemical parameters. One hundred and sixty white Pekin ducklings—one day old—were randomly divided into four equal groups. Group (1) was kept as normal control. Group (2) was inoculated I/P with 10?mg glycyrrhizin/kg BW, three times per week for four weeks. Group (3) was inoculated I/M with 0.5?ml of live attenuated DHV vaccine. Group (4) was inoculated with both glycyrrhizin (10?mg/kg BW I/P, three times per week for four weeks) and live attenuated DHV vaccine (0.5?ml, I/M). Then, all groups of treatment were challenged using virulent DHV except for 20 ducklings from the normal control group which were continued to be kept as negative control. The results revealed that duck hepatitis virus (DHV) caused macrocytic hypochromic anemia, leukopenia, hypoproteinemia, hypoalbuminemia, hyperglycemia, hypercholesterolemia, and marked elevation of liver enzymes and renal parameters. In conclusion, glycyrrhizin injected alone or in combination with DHV vaccine protected or ameliorated the deteriorating effects induced by DHV vaccine and/or duck hepatitis virus infection by improvement of erythrogram and leukogram, as well as liver and kidney functions.

Okda, Faten A.; Yassein, Safaa; Ahmed, Alaa R.; Nasr, Soad M.

2013-01-01

265

Influence of sex, source, health status and acclimation on the haematology of Clarias gariepinus (Burch,1822)  

Microsoft Academic Search

The influence of sex, source (pond and wild) acclimation and health status on some blood parameters of C. gariepinus was studied. There were no significant differences between the blood parameters (haemoglobin (Ht), packed cell volume (PCV), erythrocyte sedimentation rate (ESR), red blood cells (RBC), RBC indices ( MCHC;, MCH: and MCV), white blood cells (WBC) and differential counts (neutrophils, lymphocytes,

G. N. O. Ezeri

266

Observing a fictitious stressful event: haematological changes, including circulating leukocyte activation.  

PubMed

The aim of this study was to assess the effect of watching a psychological stressful event on the activation of leukocytes in healthy human volunteers. Blood samples were obtained from 32 healthy male and female subjects aged between 20 and 26 years before, during and after either watching an 83-minute horror film that none of the subjects had previously seen (The Texas Chainsaw Massacre, 1974) or by sitting quietly in a room (control group). Total differential cell counts, leukocyte activation as measured by the nitroblue tetrazolium (NBT) test, heart rate and blood pressure (BP) measurements were taken at defined time points. There were significant increases in peripheral circulating leukocytes, the number of activated circulating leukocytes, haemoglobin (Hb) concentration and haematocrit (Hct) in response to the stressor. These were accompanied by significant increases in heart rate, systolic and diastolic BP (P<0.05 from baseline). This is the first reported study on the effects of observing a psychologically stressful, albeit fictitious event on circulating leukocyte numbers and the state of leukocyte activation as determined by the nitrotetrazolium test. PMID:12637206

Mian, Rubina; Shelton-Rayner, Graham; Harkin, Brendan; Williams, Paul

2003-03-01

267

Diagnosis of invasive fungal infections in hematology and oncology--guidelines from the Infectious Diseases Working Party in Haematology and Oncology of the German Society for Haematology and Oncology (AGIHO).  

PubMed

Invasive fungal infections (IFIs) are a primary cause of morbidity and mortality in patients with hematological malignancies. Establishing a definite diagnosis of IFI in immunocompromised patients is particularly challenging and time consuming, but delayed initiation of antifungal treatment increases mortality. The limited overall outcome has led to the strategy of initiating either 'empirical' or 'preemptive' antifungal therapy before the final diagnosis. However, diagnostic procedures have been vastly improved in recent years. Particularly noteworthy is the introduction of newer imaging techniques and non-culture methods, including antigen-based assays, metabolite detection and molecular detection of fungal DNA from body fluid samples. Though varying widely in cancer patients, the risk of IFI is highest in those with allogeneic stem cell transplantation and those with acute leukemia. The AGIHO presents recommendations for the diagnosis of IFIs with risk-adapted screening concepts for febrile episodes in patients with haemato-oncological disorders. PMID:21948809

Ruhnke, M; Böhme, A; Buchheidt, D; Cornely, O; Donhuijsen, K; Einsele, H; Enzensberger, R; Hebart, H; Heussel, C P; Horger, M; Hof, H; Karthaus, M; Krüger, W; Maschmeyer, G; Penack, O; Ritter, J; Schwartz, S

2011-09-23

268

Retrospective study of the epidemiological, clinical, haematological and biochemical findings in 109 dogs poisoned by Vipera xanthina palestinae  

Microsoft Academic Search

One hundred and nine dogs were diagnosed as having been poisoned by viper (Vipera xanthina palestinae) venom between 1989 and 1996. Most of the cases occurred between April and September (86.2 per cent), with peaks in May (25.7 per cent) and July (20.2 per cent), and very few between November and February (3.6 per cent). Forty-two per cent of the

I. Aroch; S. Harrus

1999-01-01

269

Haematologic values of Hyla rabbits fed different levels of by-product arising from the propagation of Pleurotus pulmonarius.  

PubMed

The nutritive influence of different levels of by-product arising from the propagation of the mushroom Pleurotus pulmonarius on the red and white blood cell count of rabbits was studied in the course of fattening. The experiment was carried out on 4 groups of male Hyla rabbits, a control group (C) and 3 experimental groups (E1, E2, E3), with 15 rabbits per group. The control group was fed a standard fodder and the three experimental groups were fed the standard mix with 10%, 20% or 30% dried by-product added, respectively. At the end of the 9th week of the experiment, blood samples were obtained by cardiac puncture, and the red blood cell (RBC) count, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), haemoglobin, haematocrit, thrombocyte count, white blood cell (WBC) count and the differential WBC were determined. The different levels of byproduct interwoven by mycelium added to the feed did not change the RBC count but significantly increased the MCV, MCH, haemoglobin concentration and the haematocrit value. The different diets had no influence on either the WBC count or the differential WBC count, but the number of thrombocytes decreased significantly. PMID:8826702

Rupi?, V; Bozac, R; Muzic, S; Romi?, Z; Liker, B

1996-01-01

270

Mucosal barrier injury: biology, pathology, clinical counterparts and consequences of intensive treatment for haematological malignancy: an overview.  

PubMed

Mucositis is an inevitable side-effect of the conditioning regimens used for haematopoietic stem cell transplantation. The condition is better referred to as mucosal barrier injury (MBI) since it is primarily the result of toxicity and is a complex and dynamic pathobiological process manifested not only in the mouth but also throughout the entire digestive tract. A model has been proposed for oral MBI and consists of four phases, namely inflammatory, epithelial, ulcerative and healing phases. A variety of factors are involved in causing and modulating MBI including the nature of the conditioning regimen, the elaboration of pro-inflammatory and other cytokines, translocation of the resident microflora and their products, for example, endotoxins across the mucosal barrier, exposure to antimicrobial agents and whether or not the haematopoietic stem cell graft is from a donor. Neutropenic typhlitis is the most severe gastrointestinal manifestation of MBI, but it also influences the occurrence of other major transplant-related complications including acute GVHD, veno-occlusive disease and systemic infections. The pathobiology, clinical counterparts and the means of measuring MBI are discussed together with potential approaches for prevention, amelioration and, perhaps, even cure. Bone Marrow Transplantation (2000) 25, 1269-1278. PMID:10871732

Blijlevens, N M; Donnelly, J P; De Pauw, B E

2000-06-01

271

THE EFFECT OF XYLAZINE AND XYLAZINE-ETORPHINE- ACEPROMAZINE COMBINATION ON SOME CLINICAL AND HAEMATOLOGICAL PARAMETERS IN IMPALA AND ELANDW  

Microsoft Academic Search

Impala (Aepyceros me\\/ampus) and eland (Taurotragus onyx) were im- mobilized with a xylazine-etorphine-acepromazine combination or with xylazine alone. Clinical observations were made and blood samples were taken at intervals to determine the drug effects on clinical and blood parameters. During the immobi- lization period heart rate and body temperature decreased, as well as numbers of circulating erythrocytes and leukocytes, and

S. DREVEMO; L. KARSTADLU

1974-01-01

272

Hexavalent chromium: Toxicity and impact on carbohydrate metabolism and haematological parameters of carp ( Cyprinus carpio L.) from Saudi Arabia  

Microsoft Academic Search

The effect of hexavalent chromium on the carp,Cyprinus carpio was assessed using static bioassay. The 96h LC50 was found to be 93.6mg · l-1. In carp exposed to sublethal concentrations of chromium (15 and 25 mg · l-1), significant depletion of liver and muscle glycogen and decreasing leucocyte counts were recorded during prolonged exposure and with increasing concentration of chromium.

A. S. Al-Akel; M. J. K. Shamsi

1996-01-01

273

Effect of caffeine supplementation on haematological and biochemical variables in elite soccer players under physical stress conditions  

PubMed Central

Objective To evaluate the effect of caffeine on white cell distribution and muscle injury markers in professional soccer players during exercise. Methods 22 male athletes completed a placebo controlled double blind test protocol to simulate a soccer match, followed by a Yo?Yo intermittent recovery test. Results Exercise caused an increase in packed cell volume that was enhanced by caffeine. Caffeine and exercise had a synergistic effect on the blood lymphocyte count, which increased by about 38% after exercise, and by an additional 35% when combined with caffeine. Caffeine promoted an exercise independent rise in circulating monocytes, and a synergistic action of exercise and caffeine was observed on segmented neutrophils. Caffeine promoted thrombocytosis. Plasma adenosine deaminase, aspartate aminotransferase, and lactate dehydrogenase concentrations were enhanced by exercise, and alanine transaminase concentration was enhanced in both groups, with a synergistic effect of caffeine. Conclusions The pronounced increase in the white cell count in the group receiving caffeine appeared to be caused by greater muscle stress and consequently more intense endothelial and muscle cell injury. The use of caffeine may augment the risk of muscle damage in athletes.

Bassini-Cameron, Adriana; Sweet, Eric; Bottino, Altamiro; Bittar, Christina; Veiga, Carlos; Cameron, Luiz-Claudio

2007-01-01

274

Invasive Candida Infections in Patients With Haematological Malignancies and Hematopoietic Stem Cell Transplant Recipients: Current Epidemiology and Therapeutic Options.  

PubMed Central

In the last decades, the global epidemiological impact of invasive candidiasis (IC) in patients with hematologic malignancies (HM) and in hematopoietic stem cell transplant (HSCT) recipients has decreased and the incidence of invasive aspergillosis exceeded that of Candida infections. The use of prevention strategies, first of all antifungal prophylaxis with triazoles, contributed to the reduction of IC in these populations as demonstrated by several epidemiological studies. However, relatively little is known about the current epidemiological patterns of IC in HM and HSCT populations, because recent epidemiological data almost exclusively derive from retrospective experiences and few prospective data are available. Several prospective, controlled studies in the prophylaxis of invasive fungal diseases have been conducted in both the HM and HSCT setting. On the contrary, most of the prospective controlled trials that demonstrated the efficacy of the antifungal drugs echinocandins and voriconazole in the treatment of candidemia and invasive candidiasis mainly involved patients with underlying conditions other than HM or HSCT. For these reasons, international guidelines provided specific indications for the prophylaxis strategies in HM and HSCT patients, whereas the recommendations on therapy of documented Candida infections are based on the results observed in the general population and should be considered with caution.

Girmenia, Corrado; Finolezzi, Erica; Federico, Vincenzo; Santopietro, Michelina; Perrone, Salvatore

2011-01-01

275

Effect of caffeine supplementation on haematological and biochemical variables in elite soccer players under physical stress conditions  

Microsoft Academic Search

Objective: To evaluate the effect of caffeine on white cell distribution and muscle injury markers in professional soccer players during exercise.Methods: 22 male athletes completed a placebo controlled double blind test protocol to simulate a soccer match, followed by a Yo-Yo intermittent recovery test.Results: Exercise caused an increase in packed cell volume that was enhanced by caffeine. Caffeine and exercise

Adriana Bassini-Cameron; Eric Sweet; Altamiro Bottino; Christina Bittar; Carlos Veiga; Luiz-Claudio Cameron; E. H M Dantas

2007-01-01

276

Measurement of immature platelets with Abbott CD-Sapphire and Sysmex XE-5000 in haematology and oncology patients.  

PubMed

Abstract Background: Measurement of immature platelets was introduced into routine diagnostics by Sysmex as immature platelet fraction (IPF) some years ago and recently by Abbott as reticulated platelet fraction (rPT). Here, we compare both methods. Methods: We evaluated the precision and agreement of these parameters between Sysmex XE-5000 and Abbott CD-Sapphire in three distinct thrombocytopaenic cohorts: 30 patients with beginning thrombocytopaenia and 64 patients with recovering platelets (PLT) after chemotherapy, 16 patients with immune thrombocytopaenia (ITP) or heparin-induced thrombocytopaenia type 2 (HIT) and 110 additional normal controls. Furthermore, we analysed, how IPF/rPT differed between these thrombocytopaenic cohorts and controls. Results: Both analysers demonstrated acceptable overall precision (repeatability) of IPF/rPT with lower precision at low PLT counts. IPF/rPT artificially increased during storage of blood samples overnight. Inter-instrument comparison showed a moderate correlation (Pearson r˛=0.38) and a systematic bias of 1.04 towards higher IPF-values with the XE-5000. IPF/rPT was highest in recovering thrombopoesis after chemotherapy and moderately increased in ITP/HIT. The normal range deduced from control samples was much narrower with CD-Sapphire (1.0%-3.8%, established here for the first time) in comparison to XE-5000 (0.8%-7.9%) leading to a smaller overlap of samples with increased PLT turnover and normal controls. Conclusions: IPF and rPT both give useful information on PLT turnover, although the two analysers only show a moderate inter-instrument correlation and have different reference ranges. A better separation of patient groups with high PLT turnover like ITP/HIT from normal controls is obtained by CD-Sapphire. PMID:23800658

Meintker, Lisa; Haimerl, Maria; Ringwald, Jürgen; Krause, Stefan W

2013-11-01

277

Haematological and phenoloxidase activity changes in the shrimp Penaeus stylirostris in relation with the moult cycle: protection against vibriosis  

Microsoft Academic Search

This paper describes the changes in the total and differential haemocyte counts during the moult cycle of the shrimp,Penaeus stylirostris. Correlative measures of phenoloxidase (PO) activity and resistance to infection against vibriosis were also investigated during the moult cycle. Three types of cells are identified: hyaline cells (HC), which are small with a big nucleus, represent 80% of the total;

GILLES LE MOULLAC; MARC LE GROUMELLEC; DOMINIQUE ANSQUER; SÉBASTIEN FROISSARD; PEVA LEVY

1997-01-01

278

Combined antifungal therapy, iron chelation and surgical resection as treatment of hepatic zygomycosis in a patient with haematological malignancy.  

PubMed

We describe the case of a 19-year-old boy with acute leukaemia who developed primary hepatic zygomycosis. The patient presented with febrile neutropenia and severe abdominal tenderness. Despite the administration of antibiotics and liposomal Amphotericin-B (L-AmB), the CT scan demonstrated an increase in the size of liver lesions. A wide surgical resection was carried out and liver specimens demonstrated a branching, filamentous fungus that was identified as Rhizomucor pusillus by both phenotypic and molecular methods. The patient was treated with L-AmB combined with posaconazole, and deferasirox was subsequently added given the potential synergistic effect of this iron chelator in combination with L-AmB. Three months after surgical intervention, an allogeneic stem-cell transplantation was successfully carried out. The present case confirms that an early surgical management combined with antifungal agents is crucial to optimise the outcome of patients with zygomycosis and the use of deferasirox is a promising alternative. PMID:19302458

Busca, Alessandro; Marmont, Filippo; Locatelli, Franco; Limerutti, Giorgio; Sorrentino, Maria Teresa; Barbui, Anna; Patrono, Damiano; Salizzoni, Mauro; David, Ezio; De Rosa, Francesco

2009-03-17

279

Comparison of carbonic anhydrase activity and other haematological values of two Eutherian and three Australian marsupial mammals.  

PubMed

1. Carbonic anhydrase activity and 2,3-diphosphoglycerate (2,3-DPG) concentration were determined in whole blood from humans (Homo sapiens), rabbits (Oryctolagus cuniculus), eastern grey kangaroos (Macropus giganteus), pademelons (Thylogale billardierii) and brush-tailed possums (Trichosurus vulpecula). 2. Marsupial blood carbonic anhydrase activity increased as species body size decreased. 3. T. billardierii haemoglobin was found to have a polymorphism which may be the same (beta 2 = histidine or glutamine) as that of M. giganteus. 4. The concentration of 2,3-DPG int e red cells of T. billardierii was approximately equal to that of the haemoglobin tetramer. Levels of 2,3-DPG in the other species were similar to those previously reported. PMID:6125313

Dodgson, S J; Holland, R A

1982-01-01

280

Physiological, haematological and histopathological responses in common carp (Cyprinus carpio L.) fingerlings fed with differently detoxified Jatropha curcas kernel meal.  

PubMed

Protein rich Jatropha curcas kernel meal is toxic. It was detoxified using heat treatment and solvent extraction. Two duration of detoxification process were investigated: shorter (30 min) and longer (60 min) and the detoxified meals so obtained were designated as J(a) and J(b) respectively. Common carp fingerlings (252 fish; 3.2+/-0.07 g) were fed with the following diets: Control containing fishmeal (FM); S(50,) J(a50) and J(b50): 50% of FM protein replaced by soybean meal (SBM), detoxified Jatropha kernel meal (DJ(a)KM and DJ(b)KM); S(75), J(a75) and J(b75): 75% of FM protein replaced by SBM, DJ(a)KM and DJ(b)KM. White blood cells count, mean cell volume and mean cell haemoglobin concentration, calcium and sodium ions and total bilirubin in blood did not differ significantly among the groups. Higher (P>0.05) RBC count was observed in plant protein fed groups compared to control group. Highest alkaline phosphatase and alanine transaminase activities in blood were observed in J(a75), which were not different (P>0.05) from those in J(a50) group, but were higher than in the other groups. No adverse histopathological changes in liver and muscle of any group were observed, but intestinal mucosa of J(a75) groups showed severe pathological lesions. The results demonstrate that Jb was completely detoxified. Since the performance of J(b50) group was similar to control group and better than the other groups, optimum inclusion level of J(b) is 50% replacement of FM protein. PMID:20457206

Kumar, Vikas; Makkar, Harinder P S; Amselgruber, Werner; Becker, Klaus

2010-05-08

281

Comparative Haematological Screening of Urban and Rural Pregnant Women Attending Antenatal Clinics in Lagos and Its Environs.  

ERIC Educational Resources Information Center

|Compared blood screening data for 200 urban and rural pregnant women in Nigeria. Found that rural subjects had a greater incidence of moderate anemia than did urban subjects, and corpuscular hemoglobin concentrations fell with increased gestational age. No relationship was found between hemoglobin counts and nutrition habits. (HTH)|

Abidoye, R. O.; Olukoya, A. A.

1993-01-01

282

Routine screening for psychological distress on an Australian inpatient haematology and oncology ward: impact on use of psychosocial services  

Microsoft Academic Search

patients need to be hospitalised to establish a diagnosis, begin treatment or receive pallia- tive care, the loss of involvement in family, work or leisure roles can compound the distress experienced. 2 Between 35% and 47% of cancer patients experience significant psychological distress, with a higher prevalence in conditions where the risk of death is increased. 3,4 Psychological distress can

Stuart J Lee; Lynda J Katona; Sue E De Bono; Katrina L Lewis

2010-01-01

283

A comparative study on the adverse effects of flunixin, ketoprofen and phenylbutazone in miniature donkeys: Haematological, biochemical and pathological findings  

Microsoft Academic Search

AIM: To evaluate the adverse effects of flunixin, ketoprofen and phenylbutazone when administered I\\/V to clinically normal miniature donkeys.METHODS: Twenty clinically normal adult (2.0–2.5 years old) male miniature donkeys weighing 113–136 kg and 0.81–0.86 m tall were randomly assigned to one of four groups, and administered either saline (n=5), 1.0 mg\\/kg flunixin (n=5), 2.2 mg\\/kg ketoprofen (n=5), or 4.4 mg\\/kg

AA Mozaffari; A Derakhshanfar; A Alinejad; M Morovati

2010-01-01

284

Allogeneic peripheral blood stem cell transplantation for haematological malignancies – an analysis of kinetics of engraftment and GVHD risk  

Microsoft Academic Search

We have carried out an analysis of 44 patients undergoing allogeneic PBSC transplants from fully HLA-matched related donors with particular emphasis on engraftment kinetics and the incidence and severity of GVHD. The recipients had a median age of 37 years (range 5–56 years), 16 patients had standard-risk disease and 28 had poor-risk disease. GVHD prophylaxis was with cyclosporin A and

G Miflin; NH Russell; RM Hutchinson; G Morgan; M Potter; A Pagliuca; J Marsh; A Bell; D Milligan; M Lumley; G Cook; I Franklin

1997-01-01

285

Effect of intermittent normobaric hypoxic exposure at rest on haematological, physiological, and performance parameters in multi-sport athletes  

Microsoft Academic Search

The aim of this study was to determine whether 3 weeks of intermittent normobaric hypoxic exposure at rest was able to elicit changes that would benefit multi-sport athletes. Twenty-two multi-sport athletes of mixed ability were exposed to either a normobaric hypoxic gas (intermittent hypoxic training group) or a placebo gas containing normal room air (placebo group). The participants breathed the

Michael John Hamlin; John Hellemans

2007-01-01

286

Mucosal barrier injury: biology, pathology, clinical counterparts and consequences of intensive treatment for haematological malignancy: an overview  

Microsoft Academic Search

Mucositis is an inevitable side-effect of the conditioning regimens used for haematopoietic stem cell transplantation. The condition is better referred to as mucosal barrier injury (MBI) since it is primarily the result of toxicity and is a complex and dynamic pathobiological process manifested not only in the mouth but also throughout the entire digestive tract. A model has been proposed

NMA Blijlevens; JP Donnelly; BE De Pauw

2000-01-01

287

Is cancer-related fatigue more strongly correlated to haematological or to psychological factors in cancer patients?  

Microsoft Academic Search

Goals of work  Cancer-related fatigue is a very frequent problem in cancer, interfering with many patients’ life activities. Anaemia is present\\u000a in a large number of fatigued patients. The aim of this study was to evaluate cancer-related fatigue, in relation with haematic\\u000a haemoglobin levels and the presence of anxiety and depression.\\u000a \\u000a \\u000a \\u000a Materials and methods  The Functional Assessment Of Chronic Illness Therapy—Fatigue scale

F. Romito; R. Montanaro; C. Corvasce; M. Di Bisceglie; V. Mattioli

2008-01-01

288

The protective effect of hydrated sodium calcium aluminosilicate against haematological, biochemical and pathological changes induced by Zearalenone in mice  

Microsoft Academic Search

Hydrated sodium calcium aluminosilicate (HSCAS), an anticaking agent for mixed feed, was added alone or simultaneously with a toxic Zearalenone (ZEN) dose to balb\\/c mice and was evaluated for its ability to restore damages induced by ZEN. The latter is a mycotoxin produced by fusarium genera; it is mainly known to induce several toxic effects such as hepatotoxicity, immunotoxicity and

Samir Abbčs; Zouhour Ouanes; Jalila ben Salah-Abbčs; Zohra Houas; Ridha Oueslati; Hassen Bacha; Omar Othman

2006-01-01

289

Comparison of haematological values in restricted?and ad libitum?fed domestic fowls: White blood cells and thrombocytes  

Microsoft Academic Search

1. White blood cells and thrombocyte values were examined in normal domestic fowls of layer and broiler strains fed restricted or ad libitum diets.2. Basophils were the only white blood cells to increase significantly in number after the period of food restriction. It is suggested that the increase may have been associated with stress.3. There were more basophils in broilers

M. H. Maxwell; G. W. Robertson; S. Spence; C. C. McCorquodale

1990-01-01

290

Cardiorespiratory, hormonal and haematological responses to submaximal cycling performed 2 days after eccentric or concentric exercise bouts  

Microsoft Academic Search

Eccentric muscle actions are known to induce delayed?onset muscle soreness (DOMS) and muscle weakness (reduced static strength and dynamic peak power output) that may persist for several days. The aim of the present study was to determine whether DOMS?inducing exercise affects physiological responses to subsequent submaximal dynamic exercise. Physiological and metabolic responses to a standardized exercise task were measured 2

Michael Gleeson; Andrew K. Blannin; Bin Zhu; Stephen Brooks; Robert Cave

1995-01-01

291

Effect of gavage treatment with pulverised Garcinia kola seeds on erythrocyte membrane integrity and selected haematological indices in male albino Wistar rats.  

PubMed

This study examines the effect of the whole seed of Garcinia kola [GKS] on various blood parameters, in adult male albino rats. Five groups, of 6 animals per group, were treated by gavage with suspensions of graded concentrations of GKS daily for 5 weeks. The animals were then sacrificed and blood was obtained for estimation of the data herein presented. Packed red cell volume [PCV], hemoglobin concentration [Hb], and red blood cell count [RBC] showed significantly [P<0.05], increased response to treatment with GKS; while the platelet and white blood cell [WBC] counts showed no corresponding increase with increasing GKS dosage. The mean red blood cell volume [MCV] and mean cell hemoglobin [MCH] levels decreased with increasing GKS dosage. Prothrombin time [PT] and activated partial thromboplastin time [APPT] were both prolonged with increased GKS dosage; while the serum lipids (cholesterol and triglycerides) decreased significantly [P<0.05] with increased GKS dosage. PMID:19826463

Ahumibe, A A; Braide, V B

2009-06-01

292

The effect of ethyl acetate extract of Ficus thonningii (Blume) leaves on erythrocyte osmotic fragility and haematological parameters in acetaminophen-treated rats  

Microsoft Academic Search

Ficus thonningii (Blume) (Moraceae) known as wild fig is a large economic tree indigenous to the savannah part of Nigeria. Its leaves have\\u000a been of interest to researchers because of its increased use in diets and folkloric medicine against liver and related problems.\\u000a The effect of the ethyl acetate extract of F. thonningii leaves on erythrocyte osmotic fragility in acetaminophen-treated

Victor Masekaven Ahur; Ifeanyi Madubunyi; Adesina Y. Adenkola; Samuel C. Udem

293

Studies on Parasitologic and Haematologic Activities of an Enaminone Derivative of 4-Hydroxyquinolin-2(1H)-one Against Murine Schistosomiasis Mansoni  

PubMed Central

The activity of a novel enaminone derivative of 4–hydroxyquinoline, BDHQ, was screened for its effectiveness against murine schistosomiasis by electron microscopy and parasitologic studies. The correlation of these studies with serum levels of IFN–gamma and IgE is described. Two groups of 10 mice each were treated with different doses of BDHQ, and their results were correlated with the control and praziquantel (PZQ)–treated groups. Parasitologic study revealed significant reduction in mature worms and tissue egg loads in BDHQ– and PZQ–treated groups, whereas immature worms revealed significant reduction in BDHQ groups only. The group treated with a higher dose of BDHQ showed significant reductions in intestinal ova count when compared with the PZQ–treated group. Ultrastructural examination of the worm revealed significant degeneration of the spines and tegument in all treated groups, while the genital system was affected in BDHQ–treated groups only. BDHQ showed considerable effect on cellular activation where serum levels of IFN–gamma were significantly increased in comparison to control, while anti–soluble worm antigen preparation (SWAP) IgE was significantly increased in comparison to both the control and PZQ–treated groups. Ultrastructural examination revealed cellular activation in buffy coat and the liver in both the BDHQ– and PZQ–treated groups in comparison to the untreated one, whereas in the bone marrow and spleen, evidence of cellular activation was remarkable in the BDHQ–treated groups. In conclusion, BDHQ exhibits high levels of activity against adult and juvenile stages of these parasites, which may be due to its mixed cellular and humoral immunologic mechanisms, as demonstrated by the significant increase of serum levels of IgE and IFN–gamma shown on electron microscopy. Therefore, our results support the comparative advantage that BDHQ has over PZQ.

El-Shennawy, Amal M.; Mohamed, Amira H.; Mohamed Abass

2007-01-01

294

Studies on parasitologic and haematologic activities of an enaminone derivative of 4-hydroxyquinolin-2(1H)-one against murine schistosomiasis mansoni.  

PubMed

The activity of a novel enaminone derivative of 4-hydroxyquinoline, BDHQ, was screened for its effectiveness against murine schistosomiasis by electron microscopy and parasitologic studies. The correlation of these studies with serum levels of IFN-gamma and IgE is described. Two groups of 10 mice each were treated with different doses of BDHQ, and their results were correlated with the control and praziquantel (PZQ)-treated groups. Parasitologic study revealed significant reduction in mature worms and tissue egg loads in BDHQ- and PZQ-treated groups, whereas immature worms revealed significant reduction in BDHQ groups only. The group treated with a higher dose of BDHQ showed significant reductions in intestinal ova count when compared with the PZQ-treated group. Ultrastructural examination of the worm revealed significant degeneration of the spines and tegument in all treated groups, while the genital system was affected in BDHQ-treated groups only. BDHQ showed considerable effect on cellular activation where serum levels of IFN-gamma were significantly increased in comparison to control, while anti-soluble worm antigen preparation (SWAP) IgE was significantly increased in comparison to both the control and PZQ-treated groups. Ultrastructural examination revealed cellular activation in buffy coat and the liver in both the BDHQ- and PZQ-treated groups in comparison to the untreated one, whereas in the bone marrow and spleen, evidence of cellular activation was remarkable in the BDHQ-treated groups. In conclusion, BDHQ exhibits high levels of activity against adult and juvenile stages of these parasites, which may be due to its mixed cellular and humoral immunologic mechanisms, as demonstrated by the significant increase of serum levels of IgE and IFN-gamma shown on electron microscopy. Therefore, our results support the comparative advantage that BDHQ has over PZQ. PMID:17435624

El-Shennawy, Amal M; Mohamed, Amira H; Abass, Mohamed

2007-01-23

295

Influence of selected Indian immunostimulant herbs against white spot syndrome virus (WSSV) infection in black tiger shrimp, Penaeus monodon with reference to haematological, biochemical and immunological changes  

Microsoft Academic Search

Immunostimulants are the substances, which enhance the non-specific defence mechanism and provide resistance against the invading pathogenic micro-organism. In order to increase the immunity of shrimps against the WSSV, the methanolic extracts of five different herbal medicinal plants like Cyanodon dactylon, Aegle marmelos, Tinospora cordifolia, Picrorhiza kurooa and Eclipta alba were selected and mixed thoroughly in equal proportion. The mixed

Thavasimuthu Citarasu; Veeramani Sivaram; Grasian Immanuel; Namita Rout; Vadivel Murugan

2006-01-01

296

Comparative Study of some Haematological Parameters in European Eel (Anguilla anguilla L., 1758) Caught from Different Regions of Ceyhan River (Adana, Turkey)  

Microsoft Academic Search

Özet: Ceyhan Nehri (Adana, Türkiye)'nin farkli bölgelerinden yakalanan avrupa yilan bali?i (Anguilla anguilla L., 1758)'nda bazi hematolojik parametrelerin kar?ila?tirmali çali?masi. Ara?tirma, 2004 yilinin yaz döneminde Ceyhan nehri'nin tarimsal, sanayi, mezbaha ve evsel atiklarinin de?arj oldu?u bölge (Büyükmangit köyü) ile ayni nehir üzerinde kurulu olan Aslanta? baraji kret alti bölgesi (Osmaniye)'nde yapilmi?tir. ?stasyonlardaki su kalitesinin olasi farkliliklarinin hematolojik parametreler üzerine kar?ila?tirmali

Tülay Altun; Fatma Çevik; Ercüment Genç

297

A comparative study of clinical manifestations, haematological and serological responses after experimental infection with Anaplasma phagocytophilum in two Norwegian sheep breeds  

Microsoft Academic Search

BACKGROUND: It has been questioned if the old native Norwegian sheep breed, Old Norse Sheep (also called Norwegian Feral Sheep), normally distributed on coastal areas where ticks are abundant, is more protected against tick-borne infections than other Norwegian breeds due to a continuously high selection pressure on pasture. The aim of the present study was to test this hypothesis in

Snorre Stuen; Lise Grřva; Erik G Granquist; Karin Sandstedt; Ingrid Olesen; Hĺvard Steinshamn

2011-01-01

298

Inhalation toxicity studies of Thimet ( Phorate) in the male swiss albino mouse, Mus musculus: II. Lung histopathology, pseudocholinesterase level and haematological studies  

Microsoft Academic Search

Sub-chronic exposure of the male swiss albino mouse, Mus musculus to the recommended field dose of Thimet (Phorate; 6728.5 mg m?3) in a whole-body inhalation chamber was studied on the 2nd, 4th, 6th, 8th, 10th and 12th week of exposure. A significant depression of pseudocholinesterase (Che) level was observed throughout the experimental period. A significant decrease in haemoglobin concentration (Hb

M Morowati

1998-01-01

299

Clinical and haematological evaluation of beta thalassaemia intermedia with increased Hb F and Hb A2 in heterozygotes: beta thalassaemia intermedia I.  

PubMed Central

Family studies were performed in 10 patients from seven different families with homozygous beta zero thalassaemia intermedia and in three patients with homozygous beta+ or compound heterozygous beta+ and beta zero thalassaemia intermedia. In nine of the 10 families at least one of the parents was found to have raised Hb A2 and Hb F. In the heterozygotes with increased Hb A2 and Hb F, the means of Hb F and MCV were significantly higher than those observed in regular Hb A2 thalassaemia heterozygotes. However, the severity of imbalance in in vitro haemoglobin synthesis was similar in these two groups. The imbalance in the alpha/non-alpha synthetic ratio was heterogeneous in the patients, being 2.1 and 4.0. Segregation of the raised Hb F from the Hb A2 beta thalassaemia determinant was found to be possible in only one of the 36 heterozygotes. This may exclude the possibility of the presence of an additional determinant responsible for the activation of the gamma chain. The G gamma/A gamma ratio of Hb F was that of the fetal type (G gamma was between 50 and 71% of the total gamma chain). The A gamma T variant of gamma chain was not detected in cis of the beta zero thalassaemia determinant characterised by increased Hb F and Hb A2. A retrospective study of 180 patients with beta thalassaemia and their parents indicated that the combined rise in Hb A2 and Hb F was more common in the heterozygous parents (11 out of 30 parents) of the patients with beta zero thalassaemia than it was in the parents of patients with beta+ thalassaemia (three out of 140 parents). The presence of increased Hb A2 and Hb F in the heterozygote may in some cases determine the relative mildness of the disease.

Altay, C; Gurgey, A

1985-01-01

300

Physiological dysfunction of the haemopoietic system in a fresh water teleost, Labeo rohita , following chronic chlordane exposure. Part I—Alterations in certain haematological parameters  

Microsoft Academic Search

Chemical pesticides are used globally on an increasing scale in copious quentities te increase agricultural output. Public health use of these pesticides accounts for about 10 per cent of annual production, the remainder being used in agriculture, forestry, horticulture and the prevention and storage of food products. Their contribution te increased agricultural production and prevention of untold misery in many

S. K. Bansal; S. R. Verma; A. K. Gupta; R. C. Dalela

1979-01-01

301

Overcoming resistance to molecularly targeted anticancer therapies: rational drug combinations based on EGFR and MAPK inhibition for solid tumours and haematologic malignancies  

PubMed Central

Accumulating evidence suggests that cancer can be envisioned as a “signaling disease”, in which alterations in the cellular genome affect the expression and/or function of oncogenes and tumour suppressor genes. This ultimately disrupts the physiologic transmission of biochemical signals that normally regulate cell growth, differentiation and programmed cell death (apoptosis). From a clinical standpoint, signal transduction inhibition as a therapeutic strategy for human malignancies has recently achieved remarkable success. However, as additional drugs move forward into the clinical arena, intrinsic and acquired resistance to “targeted” agents becomes an issue for their clinical utility. One way to overcome resistance to targeted agents is to identify genetic and epigenetic aberrations underlying sensitivity/resistance, thus enabling the selection of patients that will most likely benefit from a specific therapy. Since resistance often ensues as a result of the concomitant activation of multiple, often overlapping, signaling pathways, another possibility is to interfere with multiple, cross-talking pathways involved in growth and survival control in a rational, mechanism-based, fashion. These concepts may be usefully applied, among others, to agents that target two major signal transduction pathways: the one initiated by epidermal growth factor receptor (EGFR) signaling and the one converging on mitogen-activated protein kinase (MAPK) activation. Here we review the molecular mechanisms of sensitivity/resistance to EGFR inhibitors, as well as the rationale for combining them with other targeted agents, in an attempt to overcome resistance. In the second part of the paper, we review MAPK-targeted agents, focusing on their therapeutic potential in hematologic malignancies, and examine the prospects for combinations of MAPK inhibitors with cytotoxic agents or other signal transduction-targeted agents to obtain synergistic anti-tumour effects.

Tortora, Giampaolo; Bianco, Roberto; Daniele, Gennaro; Ciardiello, Fortunato; McCubrey, James A; Ricciardi, Maria Rosaria; Ciuffreda, Ludovica; Cognetti, Francesco; Tafuri, Agostino; Milella, Michele

2007-01-01

302

The effect of the combination of acids and tannin in diet on the performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs  

Microsoft Academic Search

BACKGROUND: The abolition of in-feed antibiotics or chemotherapeutics as growth promoters have stimulated the swine industry to look for alternatives such as organic acids, botanicals, probiotics and tannin. The objective of the present study was to compare the effects of a combination of acids and tannin with diet with organic acids and diet without growth promoters on the growth performance

Marina Štukelj; Zdravko Valen?ak; Mladen Krsnik; Alenka Nemec Svete

2010-01-01

303

Parenteral nutrition following intensive cytotoxic therapy: an exploratory study on the need for parenteral nutrition after various treatment approaches for haematological malignancies  

Microsoft Academic Search

Patients receiving intensive cytotoxic therapy are traditionally supported with parenteral nutrition (PN), although it is unclear whether all patients benefit from PN. This study aimed to identify regimen-associated differences in PN requirements, to reveal discrepancies between the number of PN indications and the frequency with which PN was actually given, and to describe characteristics of patients who met nutritional goals

JA Iestra; WE Fibbe; AH Zwinderman; JA Romijn; D Kromhout

1999-01-01

304

Three-day treatment with imipenem for unexplained fever during prolonged neutropaenia in haematology patients receiving fluoroquinolone and fluconazole prophylaxis: A prospective observational safety study  

Microsoft Academic Search

BackgroundGuidelines advocate >7d of broad-spectrum antibiotics for unexplained fever (UF) during neutropaenia. However, effective antimicrobial prophylaxis reduces the incidence of gram-negative infections, which may allow shorter treatment. This study evaluates the safety of discontinuing empirical broad-spectrum antibiotics if no microbial source is documented after an initial work-up of 72h.

Lennert Slobbe; Loes van der Waal; Lydia R. Jongman; Pieternella J. Lugtenburg; Bart J. A. Rijnders

2009-01-01

305

FEED INTAKE, WEIGHT GAIN AND HAEMATOLOGY IN NILI-RAVI BUFFALO HEIFERS FED ON MOTT GRASS AND BERSEEM FODDER SUBSTITUTED WITH SALTBUSH (ATRIPLEX AMNICOLA)  

Microsoft Academic Search

A feeding management experiment was conducted to determine the effect of Mott grass and berseem fodder substituted with saltbush on the performance of Nili-Ravi buffalo heifers. Fifteen buffalo heifers of 8 months age and 120 kg average initial body weight were divided into five equal groups and fed on T1, T2, T3, T4 and T5 having Mott (Pennisetum purpurium), Berseem

J. A. BHATTI; M. YOUNAS; M. ABDULLAH; M. E. BABAR; H. NAWAZ

2009-01-01

306

Effects of maternal vitamin B12 deficiency from end of gestation to weaning on the growth and haematological and immunological parameters in mouse dams and offspring  

Microsoft Academic Search

Vitamin B12-deficiency may induce specific symptoms as neurological alterations and unspecific symptoms such as anaemia and growth retardation. In this study, maternal vitamin B12 deficiency from end of gestation to weaning was evaluated in mouse dams, which was provoked by feeding a vitamin B12-deficient diet. The animals were divided into two groups (control and deficient). The control group received the

Verónica Molina; Marta Medici; María Pía Taranto

2008-01-01

307

Physiological and haematological indices suggest superior heat tolerance of white-coloured West African Dwarf sheep in the hot humid tropics.  

PubMed

Coat colour contributes to physiological adaptation in mammals and mediates response to thermal stress. Twenty-four adult West African Dwarf sheep of both sexes and with different coat colour types were used in this study. We measured rectal temperature (RT), respiratory rate (RR) and pulse rate (PR) before sunrise and sunset during the late dry season (January-March) and early rainy season (April-June) as well as packed cell volume (PCV), red blood cell (RBC) count, white blood cell (WBC) count, plasma sodium (Na(+)) and potassium (K(+)). Animals with black coat colour had the highest (P < 0.05) mean values of 38.92 ± 0.03 °C, 65.09 ± 1.06 breaths/min, 81.35 ± 0.78 beats/min, 1.70 ± 0.01 for RT, RR, PR and heat stress index (HSI), respectively, followed by brown mouflon and brown with extensive white, while the Badger Face coloured sheep had the least mean values. There were significant (P < 0.05) differences between male and female sheep for RT, RR, PR and HSI. Season had a significant (P < 0.05) effect on RT, RR, PR and HSI. Coat colour and sex also significantly (P < 0.01) affected RBC, WBC, Na(+) and K(+). Seasonal variation (P < 0.05) in all the blood parameters was observed, with the exception of PCV. Interaction effect of coat colour and sex was significant (P < 0.05) on RT and HSI. Correlation coefficients among the measured traits ranged from positive to negative values. These results indicate that selection of white-coloured sheep to attenuate heat stress is desirable in the hot humid tropics. PMID:22639037

Fadare, Adelodun O; Peters, Sunday O; Yakubu, Abdulmojeed; Sonibare, Adekayode O; Adeleke, Matthew A; Ozoje, Michael O; Imumorin, Ikhide G

2012-05-26

308

Haematological Effect of Using Jackbean (Canavalia ensiformis (L.) D.C) Seed Meal as An Alternative Protein Source for Clarias gariepinus (Burchell, 1822)  

Microsoft Academic Search

Jackbean seed meal has crude protein and amino acid profile that recommend it for use as a substitute for fish meal in fish feed. It however, has some anti-nutritional factors some of which can be reduced to a large extend by processing (Udedibie, 1990). The study was carried out to evaluate the effect of Jackbean Seed Meal (JBSM) on the

D. I Osuigwe; A. I. Obiekezie; Johnny O. Ogunji

309

The XmnI polymorphic site 5' to the gene G(?) in a Brazilian patient with sickle cell anaemia - fetal haemoglobin concentration, haematology and clinical features.  

PubMed

We report a 20-year-old female with sickle cell anaemia and with an HbF concentration of 15.8%. The patient was not using hydroxyurea and was not receiving regular blood transfusions. The patient never had chronic manifestations of sickle cell anaemia, only pain crises of a mild intensity. After laboratory tests, we found that she was homozygous for HbS with the Bantu/atypical haplotype, and was heterozygous for the XmnI site. The influence of the XmnI site on the expression of HbF can explain the amelioration in clinical features in this haplotype association in a case of sickle cell anaemia. PMID:22419945

Belini Júnior, Edis; Cançado, Rodolfo D; Domingos, Claudia R B

2010-10-26

310

The XmnI polymorphic site 5' to the gene G? in a Brazilian patient with sickle cell anaemia - fetal haemoglobin concentration, haematology and clinical features  

PubMed Central

We report a 20-year-old female with sickle cell anaemia and with an HbF concentration of 15.8%. The patient was not using hydroxyurea and was not receiving regular blood transfusions. The patient never had chronic manifestations of sickle cell anaemia, only pain crises of a mild intensity. After laboratory tests, we found that she was homozygous for HbS with the Bantu/atypical haplotype, and was heterozygous for the XmnI site. The influence of the XmnI site on the expression of HbF can explain the amelioration in clinical features in this haplotype association in a case of sickle cell anaemia.

Cancado, Rodolfo D.; Domingos, Claudia R.B.

2010-01-01

311

Congress report: 49th Annual Meeting of the American Society of Haematology, Georgia World Congress Center, December 8–11 2007, Atlanta, Georgia  

Microsoft Academic Search

Summary  This report dwells on selected abstracts presented at the 49th Annual Meeting of the American Society of Hematology (ASH), which was held at the Georgia World Congress Center in Atlanta,\\u000a Georgia in December 2007. The aim of this report is not to discuss the very details of distinct scientific questions or clinical\\u000a problems but rather to inform the practising haematologist

S. Schmidt; M. Steurer

2008-01-01

312

Preventive effects of Egyptian sweet marjoram (Origanum majorana L.) leaves on haematological changes and cardiotoxicity in isoproterenol-treated albino rats.  

PubMed

We made an attempt to evaluate/compare the cardioprotective activity of two different doses (50 and 100 mg/kg body weight, given orally for 30 consecutive days) of Egyptian sweet marjoram leaf powder (MLP) and marjoram leaf aqueous extract (MLE) against isoproterenol (ISO)-induced myocardial infarcted rats (150 mg/kg body weight, twice at an interval of 24 h on days 29 and 30). The present study showed (probably for the first time) that both MLP and MLE (especially the high dose) significantly alleviated (P < 0.05-0.001) erythrocytosis, granulocytosis, thrombocytosis, shortened clotting time, the increase in relative heart weight, myocardial oxidative stress and the leakage of heart enzymes (creatine phosphokinase (CPK), CPK-MB isoenzyme, lactate dehydrogenase and aminotransferase) in ISO-treated rats through reactivating non-enzymic (reduced glutathione) and enzymic (catalase, glutathione peroxidase, glutathione S-transferase, superoxide dismutase) antioxidant defence system and inhibiting the production of nitric oxide and lipid peroxidation in heart tissues. The modulatory effects of marjoram leaves shown in the present study were dose-dependent in most cases and much higher in MLE (4.3-20.3 % for all parameters taken together). In addition, the doses used in the present study were considered safe. In conclusion, this study may have a significant impact on myocardial infarcted patients. PMID:23054890

Ramadan, Gamal; El-Beih, Nadia M; Arafa, Nadia M S; Zahra, Mai M

2013-06-01

313

Hygienic safety of reusable tap water filters (Germlyser®) with an operating time of 4 or 8 weeks in a haematological oncology transplantation unit  

Microsoft Academic Search

BACKGROUND: Microbial safe tap water is crucial for the safety of immunosuppressed patients. METHODS: To evaluate the suitability of new, reusable point-of-use filters (Germlyser®, Aquafree GmbH, Hamburg, Germany), three variations of a reusable filter with the same filter principle but with different outlets (with and without silver) and inner surface coating of the filter encasements (with and without nano-crystalline silver)

Georg Daeschlein; William H Krüger; Christian Selepko; Markus Rochow; Gottfried Dölken; Axel Kramer

2007-01-01

314

[Improvement of the performances of the messages for the identification of the lymphocyte population with the Beckman Coulter LH750 haematology analyser].  

PubMed

We have compared the efficiency of the detection for lymphocyte abnormalities using the LH 750 analyzer according to standard CLSI recommendations (Clinical and Laboratory Standards Institute) and three new rules dedicated to abnormal lymphocyte recognition. These new rules are defined by combining quantitative and qualitative criteria. They improve the detection of lymphocytes with abnormal morphology with an efficiency of 97.7% as compared to 81% with the standard protocol and a sensitivity of 92.8% compared to 23% initially. Regarding the detection of large granular lymphocytes, the new rules demonstrate an efficiency of 97.6%, a specificity of 81.8% and a specificity of 98.2%. These new criterias, which can be easily implemented in the settings of the automatic counter improve the technical efficiency of the validation process with an expected decrease of the number of manual slide reviews. PMID:19189889

Hue, M; Jung, G; Drénou, B

315

Major improvement of the reference method of the French drug resistance network for P-glycoprotein detection in human haematological malignancies  

Microsoft Academic Search

The aim of this study was to improve significantly the sensitivity and specificity of the flow cytometric assay of P-glycoprotein (Pgp) implemented and validated by the laboratories of the French Drug Resistance Network [Huet S, Marie JP, Gualde N, Robert J. Reference method for detection of Pgp mediated multidrug resistance in human hematological malignancies: a method validated by the laboratories

Sylvie Huet; Jean-Pierre Marie; Armelle Laurand; Jacques Robert

2005-01-01

316

Outcomes of haematopoietic stem cell transplantation for inherited metabolic disorders: a report from the Australian and New Zealand Children's Haematology Oncology Group and the Australasian Bone Marrow Transplant Recipient Registry.  

PubMed

We report a retrospective analysis of 53 haematopoietic stem cell transplants for inherited metabolic disorders performed at ANZCHOG transplant centres between 1992 and 2008. Indications for transplant included Hurler syndrome, ALD, and MLD. The majority of transplants utilized unrelated donor stem cells (66%) with 65% of those being unrelated cord blood. Conditioning therapy was largely myeloablative, with Bu plus another cytotoxic agent used in 89% of recipients. Primary graft failure was rare, occurring in three patients, all of whom remain long-term survivors following the second transplant. The CI of grade II-IV and grade III-IV acute GVHD at day +100 was 39% and 14%, respectively. Chronic GVHD occurred in 17% of recipients. TRM was 12% at day +100 and 19% at one yr post-transplant. OS at five yr was 78% for the cohort, 73% for patients with ALD and 83% for patients with Hurler syndrome. There was no statistically significant difference in overall survival between unrelated marrow and unrelated cord blood donor groups. The development of interstitial pneumonitis was an independent variable shown to significantly impact on TRM and OS. In summary, we report a large cohort of patients with inherited metabolic disorders with excellent survival post-allogeneic transplant. PMID:23802616

Mitchell, R; Nivison-Smith, I; Anazodo, A; Tiedemann, K; Shaw, P J; Teague, L; Fraser, C J; Carter, T L; Tapp, H; Alvaro, F; O'Brien, T A

2013-06-27

317

Vitamin B12 Supplementation to Mink (Mustela vison) in the Prevention of Feed-Induced Iron Deficiency Anaemia: II. Effect on Haematological Parameters and Mineral Content of the Liver  

Microsoft Academic Search

An effect of oral vitamin B12 administration on growth performance and fur quality characteristics in mink kits fed an anaemiogenic diet has earlier been found. The present data confirmed that kits fed the unsupplemented anaemiogenic diet developed anaemia with low haemoglobin, haematocrit and erythrocyte counts, microcytosis, anisocytosis, and low iron and cobalt contents in the liver. Oral vitamin B12 supplementation,

Anne-Helene Tauson; Maria Neil

1993-01-01

318

Tumour lysis syndrome and acute kidney injury in high-risk haematology patients in the rasburicase era. A prospective multicentre study from the Groupe de Recherche en Réanimation Respiratoire et Onco-Hématologique.  

PubMed

In tumour lysis syndrome (TLS), metabolic alterations caused by the destruction of malignant cells manifest as laboratory abnormalities with (clinical TLS) or without (laboratory TLS) organ dysfunction. This prospective multicentre cohort study included 153 consecutive patients with malignancies at high risk for TLS (median age 54 years (interquartile range, 38-66). Underlying malignancies were acute leukaemia (58%), aggressive non-Hodgkin lymphoma (29.5%), and Burkitt leukaemia/lymphoma (12.5%). Laboratory TLS developed in 17 (11.1%) patients and clinical TLS with acute kidney injury (AKI) in 30 (19.6%) patients. After adjustment for confounders, admission phosphates level (odds ratio [OR] per mmol/l, 5.3; 95% confidence interval [95% CI], 1.5-18.3), lactic dehydrogenase (OR per x normal, 1.1; 95%CI, 1.005-1.25), and disseminated intravascular coagulation (OR, 4.1; 95%CI, 1.4-12.3) were associated with clinical TLS; and TLS was associated with day-90 mortality (OR, 2.45; 95%CI, 1.09-5.50; P = 0.03). In this study, TLS occurred in 30.7% of high-risk patients. One third of all patients experienced AKI, for which TLS was an independent risk factor. TLS was associated with increased mortality, indicating a need for interventional studies aimed at decreasing early TLS-related deaths in this setting. PMID:23772757

Darmon, Michael; Vincent, François; Camous, Laurent; Canet, Emmanuel; Bonmati, Caroline; Braun, Thorsten; Caillot, Denis; Cornillon, Jérôme; Dimicoli, Sophie; Etienne, Anne; Galicier, Lionel; Garnier, Alice; Girault, Stéphane; Hunault-Berger, Mathilde; Marolleau, Jean-Pierre; Moreau, Philippe; Raffoux, Emmanuel; Recher, Christian; Thiebaud, Anne; Thieblemont, Catherine; Azoulay, Elie

2013-06-15

319

Guideline on the selection and use of therapeutic products to treat haemophilia and other hereditary bleeding disorders. A United Kingdom Haemophilia Center Doctors' Organisation (UKHCDO) guideline approved by the British Committee for Standards in Haematology.  

PubMed

Evidence-based guidelines are presented on the selection and use of therapeutic products to treat haemophilia and other hereditary bleeding disorders. They include details of therapeutic products available in the UK and they update and replace previous United Kingdom Haemophilia Centre Doctors' Organisation guidelines. PMID:18422612

Keeling, D; Tait, C; Makris, M

2008-04-04

320

Effect of 12-week vanadate and magnesium co-administration on chosen haematological parameters as well as on some indices of iron and copper metabolism and biomarkers of oxidative stress in rats.  

PubMed

Changes in some blood parameters after 12-week administration of sodium metavanadate (SMV; 0.125mgV/ml) or/and magnesium sulphate (MS; 0.06mgMg/ml) in drinking water were studied in outbred male Wistar rats (16 rats/each group) to explore the probable mechanism(s) underlying SMV toxicity and check whether Mg at the level selected during SMV co-administration can protect, at least in part, from a possible deleterious action of SMV. Exposure to SMV alone and in combination with MS (a) led to a decrease in fluid and food intake and body weight gain; (b) predisposed the animals to the development of microcytic-hypochromic anaemia (with excessive liver and spleen Fe deposition, unaltered plasma Fe level and enhanced Zn concentration in the erythrocytes (RBCs) characterized by a reduced haematocrit (Ht) index and haemoglobin (Hb) level, unchanged erythrocyte and reticulocyte count, anisocytosis, lowered total iron binding capacity (TIBC) and elevated transferrin saturation (TS); (c) disturbed Cu homeostasis, but (d) did not influence the leukocyte count and the plasma total antioxidant status (TAS). We suggest that abnormal metabolism and accumulation of Fe as well as an altered Cu status and the RBC Zn level might lead to defective Fe utilization and be a factor promoting the development of Fe-utilization anaemia. The disturbances in the antioxidative capacity reported previously in rats' RBCs after SMV intoxication (?cibior, Zaporowska, Environ. Toxicol. Pharmacol. 30 (2010) 153-161) may suggest that oxidative stress (OS) could also be, in part, involved in the mechanism responsible for the development of anaemia. The Mg dose ingested in combination with V under SMV-MS co-administration (a) was able to decrease, to some extent, the V concentration in the blood, (b) normalized the RBC Mg and Fe levels and (c) restored the values of some parameters of the Fe status near the control values. These results allow a supposition that a higher Mg dose consumed during SMV exposure could have better protective potential and be more effective in limiting the SMV toxicity observed. PMID:22561110

Scibior, Agnieszka; Adamczyk, Agnieszka; Go??biowska, Dorota; Nied?wiecka, Irmina

2012-04-16

321

Novel immunochemotherapy approaches towards improved targeting of Multiple Myeloma  

Microsoft Academic Search

Multiple myeloma (MM) is a haematological cancer originating from antibody producing plasma cells. Clinical symptoms may include bone pain, infections, renal failure and fatigue due to anemia. MM has an important worldwide clinical impact: it accounts for 10% of all haematological disorders and 1% of all cancers. Despite several achievements in treatment modalities in the past years, MM is still

M. S. van der Veer

2012-01-01

322

Characterization of acute promyelocytic leukemia cases lacking the classic t(15;17): results of the European Working Party. Groupe Français de Cytogénétique Hématologique, Groupe de Français d'Hematologie Cellulaire, UK Cancer Cytogenetics Group and BIOMED 1 European Community-Concerted Action "Molecular Cytogenetic Diagnosis in Haematological Malignancies".  

PubMed

Acute promyelocytic leukemia (APL) is typified by the t(15;17), generating the PML-RAR alpha fusion and predicting a beneficial response to retinoids. However, a sizeable minority of APL cases lack the classic t(15;17), prompting the establishment of the European Working Party to further characterize this group. Such cases were referred to a workshop held in Monza, Italy and subjected to morphologic, cytogenetic, and molecular review, yielding 60 evaluable patients. In the majority (42 of 60), molecular analyses revealed underlying PML/RAR alpha rearrangements due to insertions (28 of 42) or more complex mechanisms, including 3-way and simple variant translocations (14 of 42). Metaphase fluorescence in situ hybridization (FISH) demonstrated that insertions most commonly led to formation of the PML-RAR alpha fusion gene on 15q. In 11 of 60 workshop patients, PLZF/RAR alpha rearrangements were identified, including 2 patients lacking the t(11;17)(q23;q21). In one case with a normal karyotype, FISH analysis revealed insertion of RAR alpha into 11q23, and PLZF-RAR alpha was the sole fusion gene formed. Two patients were found to have t(5;17), one with a diffuse nuclear NPM staining pattern and with NPM-RAR alpha and RAR alpha-NPM transcripts detected. In the other with an unbalanced der(5)t(5;17)(q13;q21) and a nucleolar NPM localization pattern, an NPM/RAR alpha rearrangement was excluded, and FISH revealed deletion of one RAR alpha allele. In the remaining 5 workshop patients, no evidence was found for a rearrangement of RAR alpha, indicating that in rare instances, alternative mechanisms could mediate the differentiation block that typifies this disease. This study highlights the importance of combining morphologic, cytogenetic, and molecular analyses for optimal management of APL patients and better understanding of the pathogenesis of the disease. (Blood. 2000;96:1297-1308) PMID:10942371

Grimwade, D; Biondi, A; Mozziconacci, M J; Hagemeijer, A; Berger, R; Neat, M; Howe, K; Dastugue, N; Jansen, J; Radford-Weiss, I; Lo Coco, F; Lessard, M; Hernandez, J M; Delabesse, E; Head, D; Liso, V; Sainty, D; Flandrin, G; Solomon, E; Birg, F; Lafage-Pochitaloff, M

2000-08-15

323

Amegakaryocytic thrombocytopenia of nutritional vitamin B12 deficiency.  

PubMed

Description of a case of amegakaryocytic thrombocytopenia due to nutritional vitamin B12 deficiency. Megakaryocytes reappeared in the bone marrow after specific treatment and other haematological responses were prompt. PMID:3407007

Ghosh, K; Sarode, R; Varma, N; Varma, S; Garewal, G

1988-04-01

324

Early and unexpectedly severe start of influenza epidemic in the Czech Republic during influenza season 2012-13.  

PubMed

A sudden increase in severe influenza has been registered in the Czech Republic since the end of 2012, with 264 cases requiring intensive care, including 51 deaths. Most patients had at least one risk factor. Severe influenza in patients with obesity, smoking and/or haematological disorders including haematological cancers was more frequent than in the pre-pandemic period. The seasonal influenza vaccination status of the cases indicates indirect efficiency of the current vaccine in preventing severe influenza. PMID:23410257

Kyncl, J; Havlickova, M; Nagy, A; Jirincova, H; Piskova, I

2013-02-07

325

Persistence of neurological damage induced by dietary vitamin B12 deficiency in infancy  

Microsoft Academic Search

A case is reported of a 14 month old boy with severe dietary vitamin B-12 deficiency caused by his mother’s vegan diet. Cinical, electroencephalography (EEG), and haematological findings are described. Cranial magnetic resonance imaging (MRI) showed severe frontal and frontoparietal cranial atrophy. Vitamin B-12 supplements led to a rapid improvement of haematological and neurological symptoms. Serum vitamin B-12 and urinary

Ursula von Schenck; Christine Bender-Götze; Berthold Koletzko

1997-01-01

326

Rituximab-induced nonspecific interstitial pneumonia like reaction in a patient with idiopathic thrombocytopenic purpura  

Microsoft Academic Search

Rituximab, a chimeric anti-CD20 IgG1 monoclonal antibody, is an effective treatment for haematological autoimmune diseases such as idiopathic thrombocytopenic purpura (ITP). A 72-year-old man was diagnosed with idiopathic thrombocytopenic purpura (ITP). After receiving 4 cycles of rituximab in one month complete response was achieved. However, three weeks after the last infusion he presented to the haematology department with fever, productive

Charalambos Protopapadakis; Katerina M. Antoniou; Argiro Voloudaki; Katerina Samara; Athanasia Proklou; Giorgos Margaritopoulos; Nikolaos M. Siafakas

2009-01-01

327

Noninfectious pulmonary complications after bone marrow transplantation  

Microsoft Academic Search

Bone marrow transplantation (BMT) is a successful and recognised treatment option for patients with a number of haematological and non-haematological malignant and non-malignant conditions. Pulmonary complications both infectious and non-infectious are common after BMT. Multiple factors are thought to contribute to pulmonary complications, including the type and duration of immunological defects produced by the underlying disease and treatment, the development

I Khurshid; L C Anderson

2002-01-01

328

Rémission complčte sous imatinib mésylate (Glivec ®) d’un syndrome hyperéosinophilique myéloprolifératif associé ŕ une mastocytose cutanée résistant ŕ l’interféron alpha  

Microsoft Academic Search

Introduction. – The idiopathic hypereosinophilic syndrome is a rare haematologic disorder difficult to treat. We report the efficacy of imatinib mesylate in this pathology.Exegesis. – An idiopathic hypereosinophilic syndrome associated with a cutaneous mastocytosis was diagnosed in a 32-year-old man presenting with pulmonary, neurological and haematological manifestations. After failure of interferon alpha and hydroxy-urea therapy, a durable and complete clinical

P Pottier; B Planchon; O Grossi

2003-01-01

329

Pyoderma gangrenosum in association with Janus kinase 2 (JAK2V617F) mutation.  

PubMed

Pyoderma gangrenosum (PG) commonly occurs in association with various haematological and inflammatory disorders. We report a new association, a Janus kinase (JAK)2 mutation, in a 63-year-old patient with PG. We hypothesise that PG occurs by direct activation of JAK along with signal transducers and activators of transcription (STAT), a common mechanism involved in the pathogenesis of inflammatory and haematological diseases. PMID:22607468

Palanivel, J A; Macbeth, A E; Levell, N J

2012-05-21

330

Case–control study of canine infection by a newly recognised Babesia microti-like piroplasm  

Microsoft Academic Search

We did a case–control study to identify risk factors for prevalent infection of dogs by a newly recognised Babesia microti-like piroplasm. Clinical manifestations and haematology of infected dogs also were described. Forty-three laboratory-based cases and 86 individually matched controls were studied. Information on clinical signs and on risk factors was collected by a questionnaire and telephone interviews. Haematology was carried

F. J. Guitián; A. T. Camacho; S. R. Telford

2003-01-01

331

An outbreak of respiratory syncytial virus infection in a bone marrow transplant unit: effect on engraftment and outcome of pneumonia without specific antiviral treatment  

Microsoft Academic Search

Immunocompromised haematological patients are at high risk for severe, often fatal, respiratory syncytial virus (RSV) pneumonia. In the 2001 winter season, 16 of 195 (8.2%) adult haematological in-patients were diagnosed with RSV infection. Eight patients had undergone stem cell transplantation. The median age was 53 years (range 20–67). A total of 11 patients had nosocomial RSV infection while the rest

A Abdallah; K E Rowland; S K Schepetiuk; L B To; P Bardy

2003-01-01

332

Clinicopathologic spectrum of cutaneous diseases in patients with hematologic malignancies with or without allogeneic bone marrow transplantation: an observational cohort study in 101 patients.  

PubMed

Aim: Objective of the study was to determine the most common cutaneous lesions in patients with haematologic malignancies observed at dermatologic consultation and to identify the impact parameters related to the haematologic condition, like disease type/duration, remission, chemotherapy and transplantation, have on skin manifestations. Methods: A total of 101 consecutive patients with onco-haematological malignancies referred for dermatological consultation over a two-year period were included in this prospective single-centre observational cohort study. Results: The most common finding was infection (19.8%), followed by drug adverse reactions (16.8%) and malignant neoplasia (11.9%). Elderly patients and those with a longer disease duration had a higher frequency of cutaneous neoplasia. Squamous cell carcinoma was the most frequent cutaneous neoplasia; three cases of melanoma were diagnosed and had a high Breslow thickness. Cutaneous involvement due to the haematological malignancies was observed in 5 patients. Common chronic dermatoses (psoriasis and eczema) were found in 10% of patients. Transplant had no effect on the percentage of infections or tumours. Conclusion: Patients with haematological malignancies have a higher incidence of adverse drug reactions with peculiar morphologic features and a lower incidence of common chronic dermatoses than patients referred for dermatological consultation by their general practitioner or other hospital services. Infectious dermatoses were less frequent than in solid organ transplanted patients. The complex variety of cutaneous lesions, the differential diagnostic pitfalls and the prognostic relevance of early skin tumour diagnosis, evidence the importance of a correct dermatological approach. PMID:24005137

Quaglino, P; Nardň, T; Fierro, M T; Massaia, M; Orsucci, L; Fava, P; Marenco, F; Marra, E; Savoia, P; Vitolo, U; Boccadoro, M; Bernengo, M

2013-10-01

333

Myeloid sarcoma of the maxillary bone.  

PubMed

Myeloid sarcoma (MS) is a malignant tumour of myeloblasts rarely occurring in the maxillary bone. The tumour may precede or be concurrent with leukaemic infiltration of the bone marrow or herald blastic transformation of a myelodysplastic syndrome or a chronic myeloproliferative disorder. Myeloid sarcoma is uncommon in the oral cavity, but it can involve the palate, gingiva, extraction socket, and cheek. Recognition and diagnosis of myeloid sarcoma involving the soft tissues of the oral cavity in an otherwise asymptomatic patient is important and mandates an appropriate haematological diagnostic workup. We herein report on a new case without any evidence of haematological disorders. We discuss the pathological diagnosis and the therapeutical approaches. PMID:16519776

Goteri, G; Ascani, G; Messi, M; Filosa, A; Segura-Egea, J J; Rubini, C; Bullon, P

2006-04-01

334

An unusual cause for recurrent jaundice in an otherwise healthy male  

PubMed Central

A 41-year-old Asian-Indian male presented with recurrent episodes of jaundice over the past six months. Physical examination was normal, barring mild icterus. Laboratory parameters revealed indirect hyperbilirubinemia. Further evaluation yielded a diagnosis of severe nutritional vitamin B12 deficiency. Indirect hyperbilirubinemia was ascribed to ineffective erythropoiesis. Underlying Gilbert's syndrome was ruled out by provocative testing with lipid-restricted diet. Presentation of severe vitamin B12 deficiency with isolated hyperbilirubinemia without concomitant major haematologic or neurologic dysfunction is unusual and potentially underdiagnosed. Awareness of this possibility can permit early diagnosis of vitamin B12 deficiency and forestall development of severe haematologic and neurologic sequelae.

Dasari, Sowjanya; Naha, Kushal; Prabhu, Mukhyaprana

2012-01-01

335

An unusual cause for recurrent jaundice in an otherwise healthy male.  

PubMed

A 41-year-old Asian-Indian male presented with recurrent episodes of jaundice over the past six months. Physical examination was normal, barring mild icterus. Laboratory parameters revealed indirect hyperbilirubinemia. Further evaluation yielded a diagnosis of severe nutritional vitamin B12 deficiency. Indirect hyperbilirubinemia was ascribed to ineffective erythropoiesis. Underlying Gilbert's syndrome was ruled out by provocative testing with lipid-restricted diet. Presentation of severe vitamin B12 deficiency with isolated hyperbilirubinemia without concomitant major haematologic or neurologic dysfunction is unusual and potentially underdiagnosed. Awareness of this possibility can permit early diagnosis of vitamin B12 deficiency and forestall development of severe haematologic and neurologic sequelae. PMID:23173018

Dasari, Sowjanya; Naha, Kushal; Prabhu, Mukhyaprana

2012-10-31

336

Corynebacterium jeikeium bacteraemia and pulmonary infiltrates in a patient with acute myelogenous leukaemia.  

PubMed

The authors used vancomycin (VCM) to treat Corynebacterium jeikeium bacteraemia and pulmonary infiltrates in a patient with acute myelogenous leukaemia. Pulmonary infiltrates are found in about one-third of patients with haematological pathologies accompanying C jeikeium bacteraemia. Although linezolid and daptomycin are antimicrobial alternatives for treating Corynebacterium infections, the authors believe that VCM is the best choice for immediate treatment success and to prevent pulmonary infiltrates from developing in the presence of bloodstream infection with Corynebacteria in patients with haematological malignancies. PMID:22605704

Sato, Kota; Uchiyama, Michihiro

2012-03-20

337

[Use of pharmacological and non-pharmacological techniques to control pain and states of suffering].  

PubMed

In 1993, World Health Organization has assumered pain like pathology and in 1998 began the spreading of guidelines specifications for cancer pain relief control and palliative care in children. Thanks to experience that we acquired in law in Oncology and Haematology paediatric Unit of "A. Meyer" Hospital of Florence, we have been invited to carry to the last congress of Florence of the AIEOP (Italian haematology-oncology paediatric association) an ours contribution in matter. So knowing the "state of the art" of the AIEOP centers has become necessary. With this work the results of such cognitive investigation will be presented. PMID:12219386

Baggiani, A; Fabbrini, F

338

[Neuropathies associated with paraproteinemia (monoclonal gammopathy)].  

PubMed

Coexistence of neuropathy and paraproteinemia (monoclonal gammopathy) is a common and complex problem seen in clinical practice and requires the distinction of specific syndromes. The clinical courses of these neuropathies are typically chronic and progressive. A precise distinction of the type of haematologic disorder associated (benign or malignant), investigation of other organs manifestations, and assessment of specific markers are mandatory. These steps are important to initiate an appropriate therapy that may include chemotherapy and/or immunosuppressive treatment targeting the neuropathy and the haematological dysfunction. PMID:23717902

Lalive, P H; Kuntzer, T; Dietrich, P-Y

2013-05-01

339

Case Report. Prevention of Clozapine-Induced Granulocytopenia/Agranulocytosis with Granulocyte-Colony Stimulating Factor (G-CSF) in an Intellectually Disabled Patient with Schizophrenia  

ERIC Educational Resources Information Center

|Background: While clozapine is an effective treatment for refractory schizophrenia, its use is limited by haematological side effects. Treatment options that allow continued prescription of clozapine by tackling these side effects will greatly aid patients for whom this medication is all too often their only hope of recovery. Method: In this case…

Rajagopal, G.; Graham, J. G.; Haut, F. F. A.

2007-01-01

340

Different aspects of thalidomide treatment and stem cell transplantation in multiple myeloma patients  

Microsoft Academic Search

Multiple myeloma (MM) is an haematological malignancy caused by an unrestrained proliferation of plasma cells (monoclonally differentiated B-cells), and part of the white blood cell count. This proliferation infiltrates the blood forming skeletal bone marrow, producing osteoclastic factors, causing local bone destruction and bone resorption, causing bone pain as one of the major symptoms. The clonally transformed plasma cells all

A. M. W. van Marion

2006-01-01

341

Killer cell Immunoglobulin-like Receptor (KIR) polymorphism in Haematopoietic Stem Cell Transplantation (HSCT) : The effect of KIR gene and genotype polymorphism on clinical outcome after HSCT  

Microsoft Academic Search

Haematopoietic stem cell transplantation (HSCT) often is a final treatment option for patients suffering from haematological malignancies and metabolic disorders. When HSCT is applied to treat leukaemia, relapse of the disease is a recurrent complication. Donor lymphocyte infusion (DLI) with T cells is then administered to eliminate these tumour cells. The disadvantage of this treatment is the increased risk of

A. J. Schellekens

2008-01-01

342

Acute admissions of patients with sickle cell disease who live in Britain  

Microsoft Academic Search

All acute admissions of patients with sickle cell disease who lived in the London borough of Brent and attended this hospital were analysed for a period of one year. Sixty three of the 211 patients who were followed up by the haematology department required 161 acute admissions during the year. Most admissions (126) were for the 42 patients with homozygous

M Brozovi?; S C Davies; A I Brownell

1987-01-01

343

Assessment of renal vascular resistance and blood pressure in dogs and cats with renal disease  

Microsoft Academic Search

This study investigated the possible relationships between renal resistive index (RI) or pulsatility index (PI) and systolic blood pressure and biochemical and haematological parameters in dogs and cats with renal disease. The study included 50 dogs and 20 cats with renal disease. RI and PI were significantly higher in both dogs and cats with renal disease than in 27 healthy

R. Novellas; R. Ruiz de Gopegui; Y. Espada

2010-01-01

344

Hepatic enzymes have a role in the diagnosis of hepatic injury after blunt abdominal trauma  

Microsoft Academic Search

IntroductionDelayed diagnosis of patients with severe liver injuries is associated with an adverse outcome. As computed tomographic (CT) scan is not always available in the management of blunt abdominal trauma worldwide, the present study was undertaken to determine the accuracy of selected haematological markers in predicting the presence of hepatic injury and its severity after blunt abdominal trauma.

Ker-Kan Tan; Shieh-Ling Bang; Appasamy Vijayan; Ming-Terk Chiu

2009-01-01

345

The Austrian Bone Marrow Donor Registry: Providing Patients in Austria with Unrelated Donors for Transplant - a Worldwide  

Microsoft Academic Search

Background: The Austrian Bone Marrow Donor Registry is the central search coordinating unit in charge of national and international donor searches in Austria. Patients and Methods: Between 1988 and 2010, a worldwide search for an unrelated donor of blood stem cells (URD) was initiated for 2,166 Austrian patients with haematological disorders, 1,671 adults and 495 children, by the Austrian Bone

M. Margit; M. Martina; A. Andrea; B. Barbara; L. Ljiljana; S. Sonja; H. Heinz; I. Ingrid; G. Gottfried

346

REGISTRI KOSTNEGA MOZGA IN REGISTRI POPKOVNI?NE KRVI DOMA IN PO SVETU BONE MARROW DONOR AND CORD BLOOD REGISTRIES IN SLOVENIA AND WORLDWIDE  

Microsoft Academic Search

Background. The use of cord blood to treat haematological malignancies, bone marrow failures and congenital immunodeficiencies is rapidly increasing. This increase led to the establishment of Cord Blood Banks which are collecting and storing cord blood units. Conclusions. HLA typing results of those units are collected in national bone marrow registries, they can internationally be searched for in Bone Marrow

Miha Tonejc

347

[Disorders of the larynx and chronic inflammatory diseases].  

PubMed

Chronic inflammatory diseases including tuberculosis, rheumatic disorders (rheumatoid arthritis, sarcoidosis, systemic lupus erythematosus, Wegeners's granulomatosis, relapsing polychondritis) and reflux disease are considered as systemic diseases, and may affect the larynx. The clinical symptoms are often unspecific, leading to prolonged intervals to diagnosis. Solid and haematological tumours should be considered in differential diagnosis and may require bioptic sampling. Treatment may require interdisciplinary approach. PMID:23044789

Pickhard, A; Smith, E; Rottscholl, R; Brosch, S; Reiter, R

2012-10-08

348

Use of V H, D and J H immunoglobulin gene segments in Brazilian patients with chronic lymphocytic leukaemia (CLL)  

Microsoft Academic Search

Chronic lymphocytic leukaemia (CLL) is a haematological malignancy for which reliable prognostic markers are needed in view of its clinical heterogeneity. In approximately 50% of CLL patients, immunoglobulin (Ig) rearrange- ments are modified by somatic hypermutation (SHM), a process that represents a reliable prognostic indicator of fa- vourable progression. In this study, we investigated SHM in 37 Brazilian CLL patients

Beatriz Jatobá Pimentel; Cláudio Gustavo Stefanoff; Aline Santos Moreira; Héctor N. Seuánez; Ilana Renault Zalcberg

2008-01-01

349

Changes in peripheral blood leukocyte populations in pigs with naturally occurring exudative epidermitis  

Microsoft Academic Search

The objective of the study was to analyze changes in peripheral blood leukocyte subsets in cases of naturally occurring exudative epidermitis (EE) in pigs. Five of ten piglets developed the chronic clinical form of EE 2–5 days after weaning (PW). Blood samples were obtained at 7, 14 and 21 days from both normal and clinically affected piglets for routine haematology

M. Nofrarías; J. Pujols; J. Segalés; X. Gibert; N. Majó

2006-01-01

350

In vitro toxicity of trichothecenes on human erythroblastic progenitors  

Microsoft Academic Search

Trichothecenes are mycotoxins produced by various species of fungi which can occur on various agricultural products. Among these compounds, T-2 toxin, HT-2 toxin, diacetoxyscirpenol (DAS) and deoxynivalenol (DON) are the most naturally encountered and potent trichothe cenes. Consumption of trichothecene contaminated foods by farm animals and humans leads to mycotoxicoses. Trichothecenes are known to induce haematologic dis orders such as

B. Rio; S. Lautraite; D. Parent-Massin

1997-01-01

351

Haematotoxicity of trichothecenes  

Microsoft Academic Search

Human toxicosis induced by consumption of foodstuffs contaminated with trichothecenes presents one common major symptom: a haematological perturbation manifesting principally as thrombocytopenia and leukopenia. The patients have rapidly progressing coagulation problems, and compromised resistance to infections. Consequently, they are subject to septicaemia and massive haemorrhages. In horses, cattle, poultry, cats, mice and guinea pigs, subacute and subchronic ingestion of trichothecenes

D. Parent-Massin

2004-01-01

352

Trichothecene toxicity on human megakaryocyte progenitors (CFU-MK)  

Microsoft Academic Search

Trichothecenes are mycotoxins produced by various species of fungi, which can occur on various agricultural products. Among these compounds, T-2 toxin, HT-2 toxin, diacetoxyscirpenol (DAS) and deoxynivalenol (DON) are the most naturally encountered and the most potent trichothecenes. Consumption of trichothecene contaminated foods by farm animals and humans leads to mycotoxicosis. Trichothecenes are known to induce haematological disorders such as

R. Froquet; Y. Sibiril; D. Parent-Massin

2001-01-01

353

Autologous haematopoietic stem cell transplants for autoimmune disease – feasibility and transplant-related mortality  

Microsoft Academic Search

This ongoing multicentre prospective phase I\\/II trial enrolled 74 consecutive patients from 22 centres worldwide with severe autoimmune disease, 35 with rheumatological disorders, 31 with neurological, five with haematological and three with vasculitides. They were treated with autologous peripheral blood or bone marrow transplants according to predetermined criteria. Two patients died after mobilisation before transplant. Seventy-two patients were given 73

A Tyndall; A Fassas; J Passweg; C Ruiz de Elvira; M Attal; P Brooks; C Black; P Durez; J Finke; S Forman; L Fouillard; D Furst; JA Holmes; D Joske; JP Jouet; I Kötter; F Locatelli; HG Prentice; AM Marmont; P McSweeney; M Musso; HH Peter; JA Snowden; K Sullivan; A Tichelli; J Vavriec; NM Wulffraat; N Schmitz; A Gratwohl

1999-01-01

354

Polymorphonuclear function in naturally occurring renal failure in dogs  

Microsoft Academic Search

Chronic renal failure causes immunosuppression in people and is thought to be one of the causes of non-infectious secondary immunosuppression in dogs. The purpose of this study was to evaluate changes in counts and activity of polymorphonuclears in dogs with chronic renal failure in various stages. Haematological, biochemical examinations and examination of non-specific immune response cells (total and differential leukocyte

S. Kralova; L. Leva; M. Toman

355

Effects of Prolonged Intravenous of Flunixin Meglumine in Healthy Dogs  

Microsoft Academic Search

Erdogan H. M., V. Gunes, H. I. Gokce, M. Uzun, M. Citil, N. Yuksek: Effects of Prolonged Intravenous of Flunixin Meglumine in Healthy Dogs. Acta Vet. Brno 2003, 72: 71-78. This study was designed to evaluate possible side effects on liver and kidney functions and haematological indices, associated with long-term intravenous (IV) administration of flunixin meglumine in healthy dogs. For

H. M. ERDOGAN; V. GUNES; H. I. GOKCE; M. UZUN; M. CITIL; N. YUKSEK

356

Characteristics of Umbilical Cord Blood Units Collected from Preterm Deliveries  

Microsoft Academic Search

Background: Umbilical cord blood (UCB) banking is a well-established activity supporting the increasing number of UCB transplantations in haematological diseases. Our aim was to analyse the UCB characteristics of UCB units from preterm deliveries and compare them to full-term deliveries. Material and Methods: A prospective study in 194 preterm deliveries occurring at the La Fe University Hospital in Valencia was

Pilar Solves; Mara López; Vicente Mirabet; Amando Blanquer; Roberto Roig; Alfredo Perales

2009-01-01

357

Clinical utility of blood tests in elite athletes with short term fatigue  

Microsoft Academic Search

Objective: To determine, in a population of elite athletes at their initial presentation with tiredness or fatigue, whether a set of haematological and biochemical investigations enhances the diagnostic process over and above the information gained from clinical history and examination.Methods: A sequential series of 50 elite athletes were studied at the initial consultation for a primary complaint of fatigue, tiredness,

K E Fallon

2006-01-01

358

The carcinogenic risk of high dose total body irradiation in non-human primates  

Microsoft Academic Search

Purpose: High dose total body irradiation (TBI) in combination with chemotherapy, followed by rescue with bone marrow transplantation (BMT), is increasingly used for the treatment of haematological malignancies. With the increasing success of this treatment and its current introduction for treating refractory autoimmune diseases the risk of radiation carcinogenesis is of growing concern. Studies on tumour induction in non-human primates

Johan J Broerse; Rolf W Bartstra; Dirk W van Bekkum; Marein H van der Hage; Chris Zurcher; Mathew J van Zwieten; Carel F Hollander

2000-01-01

359

Neurophysiological and neuropathological characterization of new murine models of chemotherapy-induced chronic peripheral neuropathies  

Microsoft Academic Search

Cisplatin, paclitaxel and bortezomib belong to some of the most effective families of chemotherapy drugs for solid and haematological cancers. Epothilones represent a new family of very promising antitubulin agents. The clinical use of all these drugs is limited by their severe peripheral neurotoxicity. Several in vivo rat models have reproduced the characteristics of the peripheral neurotoxicity of these drugs.

V. A. Carozzi; A. Canta; N. Oggioni; B. Sala; A. Chiorazzi; C. Meregalli; M. Bossi; P. Marmiroli; G. Cavaletti

2010-01-01

360

Measuring Treatment Preferences and Willingness to Pay for Docetaxel in Advanced Ovarian Cancer  

Microsoft Academic Search

Background: Docetaxel is an equally active alternative to paclitaxel in advanced ovarian cancer but has a different adverse effect profile. Whilst paclitaxel is associated with less haematological toxicity, such as febrile neutropenia and anaemia, docetaxel causes less sensory and motor neuropathy. Objective: To measure the economic value and preference scores for docetaxel as an alternative to paclitaxel in patients with

George Dranitsaris; Julia Elia-Pacitti; Wayne Cottrell

2004-01-01

361

Effects of Clove Oil Anaesthesia on European Catfish (Silurus glanis L.)  

Microsoft Academic Search

Velí‰ek J., T. Wlasow, P. Gomulka, Z. Svobodová, L. Novotn?, E. Ziomek: Effects of Clove Oil Anaesthesia on European Catfish (Silurus glanis L.). Acta Vet. Brno 2006, 75: 99-106. The aim of the study was to investigate acute toxicity of clove oil for European catfish and, using values of haematological and biochemical profiles of blood and histological tissue examinations, to

J. Velíšek; T. Wlasow; P. Gomulka; Z. Svobodová; L. Novotný; E. Ziomek

2006-01-01

362

Effects of Clove Oil Anaesthesia on Rainbow Trout (Oncorhynchus mykiss)  

Microsoft Academic Search

Velí‰ek J., Z. Svobodová, V. Piaăková: Effects of Clove Oil Anaesthesia on Rainbow Trout (Oncorhynchus mykiss). Acta Vet. Brno 2005, 74: 139-146. The aim of the study was to investigate acute toxicity of clove oil for rainbow trout and, using values of haematological and biochemical profiles of blood and histological tissue examinations, to assess the effects of the fish exposure

Z. SVOBODOVÁ; V. PIAâKOVÁ

2005-01-01

363

Migration of bone marrow stem cells in ischaemic brain  

Microsoft Academic Search

Stem cell therapy has been demonstrated to be effective in the management of haematological malignancy and solid cancer, but its role in neurodegenerative conditions remains uncertain. We hypothesize that: (1) ventricular delivery of bone marrow stem cells improves functional outcome in experimental ischaemia of the mouse brain; and (2) this improved outcome is due to migration of bone marrow stem

W. S. Poon; G. Lu; K. S. Tsang; X. L. Zhu; G. G. Chen; H. K. Ng

364

Oncogenic tyrosine kinases and the dna-damage response  

Microsoft Academic Search

Oncogenic tyrosine kinases (OTKs) are involved in the induction of many types of tumour, including haematological malignancies and cancers of the breast, prostate, colon and lung. Neoplastic cells that express OTKs are usually resistant to apoptosis that is induced by DNA-damaging agents, such as cytostatic drugs and irradiation, and they display genomic instability. So, what are the mechanisms involved, and

Tomasz Skorski

2002-01-01

365

Carboxylesterase 1 (Ces1): from monocyte marker to major player  

Microsoft Academic Search

There are few, if any, enzymes that have been studied by, and have importance in, so many and varied disciplines as has monocyte esterase\\/Ces 1. In this review its involvement in the fields of histochemistry, haematology, toxicology, pharmacology, therapeutics, and tumour cell killing, immune surveillance and malignant disease, is briefly described.

Geraldine M Markey

2010-01-01

366

[Self-hypnosis, a resource for children undergoing painful treatment].  

PubMed

Learning self-hypnosis enables children to partially or totally manage the sensory and emotional components of pain, especially that linked to the use of a central line in paediatric onco-haematology. Specially trained paediatric nurses can teach this special technique. PMID:20518243

Restif, Anne-Sophie

367

Erythrocyte Vitamin B12 Activity in Lactovegetarian Pregnant Indian Women  

Microsoft Academic Search

Plasma and erythrocyte vitamin B12 levels were studied in pregnant Indian women consuming very little food of animal origin and compared with healthy non-pregnant women from the same dietetic group. The comparison of the mean values for routine haematological parameters revealed distinct fall in haemoglobin, packed cell volume, plasma vitamin B12, and albumin levels in pregnant women. However, the mean

V. S. Jathar; A. B. Inamdar-Deshmukh

1981-01-01

368

Neurological changes in fruit bats deficient in vitamin B12  

Microsoft Academic Search

HAEMATOLOGICAL and neurological sequelae are the two major effects of vitamin B12 deficiency in man. In the haemopoietic system, megaloblastic change leading to anaemia seems to be caused by deranged DNA synthesis1. The cause of central nervous system demyelination, however, is unknown and progress in this area has been hampered by the lack of a suitable experimental animal model. We

Ralph Green; Susan V. van Tonder; G. Julien Oettle; Gillian Cole; Jack Metz

1975-01-01

369

The Contributions of Paul Ehrlich to Pharmacology: A Tribute on the Occasion of the Centenary of His Nobel Prize  

Microsoft Academic Search

On the centenary of Paul Ehrlich’s Nobel Prize, this German researcher deserves to be remembered as a pioneer in a large number of scientific disciplines. As a result of his enthusiasm and scientific abilities, dedication, and contacts with other scientists of his time, he was able to make countless contributions in fields as diverse as histology, haematology, immunology, oncology, microbiology

Fčlix Bosch; Laia Rosich

2008-01-01

370

Causes of ischaemic stroke in the young  

Microsoft Academic Search

The causes of ischaemic stroke in young adults are many and diverse. Such patients usually require more extensive investigations in order to find an underlying cause than more elderly patients. It is important that a comprehensive search is made since many of the underlying disorders are treatable. Principal causes are extracranial arterial dissection, cardioembolism, premature atherosclerosis, haematological and immunological disorders

P. J. Martin; T. P. Enevoldson; P. R. Humphrey

1997-01-01

371

Comparison of Pigeon Guillemot, Cepphus columba, Blood Parameters from Oiled and Unoiled Areas of Alaska Eight Years After the Exxon Valdez Oil Spill  

Microsoft Academic Search

In 1997, we compared the haematological and plasma biochemical profiles among populations of pigeon guillemots, Cepphus columba, in areas oiled and not oiled by the 1989 Exxon Valdez oil spill (EVOS) that occurred in Prince William Sound (PWS), Alaska. Pigeon guillemot populations in PWS were injured by EVOS and have not returned to pre-spill levels. If oil contamination is limiting

Pamela E Seiser; Lawrence K Duffy; A David McGuire; Daniel D Roby; Gregory H Golet; Michael A Litzow

2000-01-01

372

Copper deficiency myeloneuropathy and pancytopenia secondary to overuse of zinc supplementation.  

PubMed

The haematological complications of acquired copper deficiency have been well documented, but the neurological complications have only recently been reported. An illustrative case of copper deficiency myeloneuropathy with pancytopenia is presented and the potential aetiologies and neurological manifestations of this deficiency state discussed. PMID:15834043

Rowin, J; Lewis, S L

2005-05-01

373

A case of kala-azar diagnosed by bone marrow aspiration  

Microsoft Academic Search

A 35-year-old man from Bangladesh, who had been in Malaysia for approximately a year, was extensively investigated formore than two months in a state hospital for pyrexia with hepatosplenomegaly . However, no obvious cause of his illness was found. He was treated with multiple antibiotics with no resolution of pyrexia and hepatosplenomegaly. He was later referred to the Haematology Unit,

Noor Hussin; Jeffrey ABU HASSAN

374

Satisfactory recovery and viability of stem cells cryopreserved at high cell concentration  

Microsoft Academic Search

Peripheral Blood Stem Cells (PBSC) are being extensively used in both oncology-haematology and in solid tumor therapy schedules. The latest generation of cell separators allow the collection of greater numbers of nucleated cells (NC) than is usually obtained in bone marrow transplantation (BMT) settings. Thus, larger volumes of components would be stored if the same NC concentration employed in BMT

P Perseghin; R Epis; M Vigano; A Malacrida; A Pastorini; G Camerone

1997-01-01

375

Androgenic steroid effects on liver and red cells  

Microsoft Academic Search

Haematological and hepatic effects of testosterone\\/anabolic steroid self-administration were investigated in five power athletes during 26 weeks of training. During steroid administration blood haematocrit had increased 9.6% (p less than .05) in the study group (n = 5), but not in the control group (n = 6). This erythropoietic phenomenon was supported by increased (p less than .05) RBC and

M Alén

1985-01-01

376

Protective effects of fruit extracts of Hippophae rhamnoides L. against arsenic toxicity in Swiss albino mice  

Microsoft Academic Search

Seabuckthorn (Hippophae rhamnoides L.) berry has a long history of applications as a food and medicinal ingredient in eastern countries. The present study was carried out to investigate the effect of different fruit extracts of H. rhamnoides on altered biochemical parameters indicative of haematological alterations, tissue oxidative stress, and arsenic concentration in arsenicexposed mice (2.5 mg\\/kg body weight, intraperitoneally). Two

R Gupta; S JS Flora

2006-01-01

377

Maduramicin and tiamulin compatibility in broiler chickens  

Microsoft Academic Search

A total of 480 1?day?old Hybro broiler chickens were divided into five treatment groups (A: unmedicated control, B: maduramicin, C: maduramicin + tiamulin, D: monensin + tiamulin and E: tiamulin) to study the effect on performance parameters, organ weights, blood haematology and biochemistry, and histopathology of liver and selected striated muscles, when maduramicin at 5 parts\\/10 and monensin at 100

J. J. Badiola; D. F. Luco; V. Pérez; M. A. Vargas; L. Luján; J. F. García Marín

1994-01-01

378

Thalassaemia in Cyprus  

Microsoft Academic Search

Frequencies of the thalassaemias in Cyprus were examined by a survey of hospital inpatients and haematological investigations of adult and newborn population samples. The data indicate that 15% of the Greek and Turkish Cypriots are carriers of beta-thalassaemia genes, while 10% of the population carry alpha-thalassaemia genes. These are the highest frequencies of thalassaemia genes found today in any Caucasian

Th. Ashiotis; Z. Zachariadis; K. Sofroniadou; D. Loukopoulos; G. Stamatoyannopoulos

1973-01-01

379

Comparison of pigeon guillemot, Cepphus columba, blood parameters from oiled and Unoiled areas of Alaska eight years after the Exxon Valdez oil spill  

Microsoft Academic Search

In 1997, we compared the haematological and plasma biochemical profiles among populations of pigeon guille- mots, Cepphus columba, in areas oiled and not oiled by the 1989 Exxon Valdez oil spill (EVOS) that occurred in Prince William Sound (PWS), Alaska. Pigeon guillemot populations in PWS were injured by EVOS and have not returned to pre-spill levels. If oil contamination is

PAMELA E. SEISER; LAWRENCE K. DUFFY; A. DAVID MCGUIRE; DANIEL D. ROBY; GREGORY H. GOLET; MICHAEL A. LITZOW

2000-01-01

380

Parasitic crustaceans of Adriatic fishes.  

PubMed

45 species of Copepoda and 14 of Isopoda have been found on the South Adriatic (Montenegrian coast) fishes during the 10 years study. In addition to the check-list, some results of pathological and haematological investigations, showing that these parasites have a considerable impact on the growth, condition index and physiological state of hosts, are given. PMID:1823479

Radujkovi?, B M

1991-01-01

381

Mutations of the Kirsten-ras proto-oncogene in human preleukaemia  

Microsoft Academic Search

The myelodysplastic syndrome (MDS) or preleukaemia is a haematological disorder characterized by low blood counts, bone marrow cells of abnormal appearance and progression to acute leukaemia in as many as 30% of patients1. The distinctive pre-leukaemic and leukaemic phases of this disease make it an attractive model for neoplastic progression in human tumours. We reasoned that, because dominantly transforming genes

Edison Liu; Brian Hjelle; Rodman Morgan; Frederick Hecht; J. Michael Bishop

1987-01-01

382

Chronic Psychosis Associated with Vitamin B12 Deficiency  

Microsoft Academic Search

B12 deficiency is widely prevalent and usually presents with haematologic and neuropsychiatric manifestations. Psychiatric symptoms seldom precede anaemia and present as the principal manifestation of B12 deficiency. A report an unusual presentation of long standing psychotic symptoms without anaemia in a 31 year old male, who presented to a tertiary care psychiatric facility. His physical examination revealed hyper pigmentation of

AP Rajkumar; P Jebaraj

2008-01-01

383

Effect of Therapy with Vitamin B12 and Folic Acid on Elderly Patients with Low Concentrations of Serum Vitamin B12 or Erythrocyte Folate but Normal Blood Counts  

Microsoft Academic Search

To help understand the haematological significance of the low concentrations of serum vitamin B12 and erythrocyte folate occurring in elderly patients, 17 acute admissions to a geriatric unit with a low concentration of serum vitamin B12 or erythrocyte folate but a normal blood count were treated with vitamin B12 and folic acid for 3 months. Bone marrow deoxyuridine supression was

J. H. Matthews; D. M. Clark; G. M. Abrahamson

1988-01-01

384

Paralysis of Upward Gaze as a Presenting Symptom of Vitamin B12 Deficiency  

Microsoft Academic Search

A 47-year-old woman presented with paralysis of upward gaze associated with Addison’s pernicious anaemia. Administration of cyanocobalamin resulted in significant improvement of eye movements and haematological parameters. Isolated paralysis of upward gaze may be a feature of vitamin B12 deficiency.Copyright © 1984 S. Karger AG, Basel

R. Sandyk

1984-01-01

385

Zoological Parks  

Microsoft Academic Search

In order to determine hematological reference values, a total of 104 blood samples were obtained from captive birds of Ara and Amazona genera in different Venezuelan zoos. Red Blood Cell (RBC), Packed Cell Volume (PCV), Mean Corpuscular Vo- lume (MCV), White Blood Cell (WBC) and leukocytes differen- tial counts were measured using manual haematological methods. Absolute leukocytes values were calculated

Mary Cruz Alvarado; Cruz Arraga-Alvarado; María Rincón Rincon; Gibson Fernández; Yenen Villasmil-Ontiveros; Orlando Gómez; Antmar Henriquez

2008-01-01

386

A three-year longitudinal study on the effects of a diet containing genetically modified Bt176 maize on the health status and performance of sheep  

Microsoft Academic Search

This study shows that a diet including insect-resistant Bt176 maize, fed to 53 ewes and their progeny for 3 years, did not have adverse effects on their health or performance and that no horizontal gene transfer to ruminal microorganisms or animal tissues was detected. No differences were observed regarding performance, reproductive traits, haematological parameters, antioxidant defences, lymphocyte proliferative capacity, phagocytosis and

Massimo Trabalza-Marinucci; Giorgio Brandi; Cristina Rondini; Luca Avellini; Camilla Giammarini; Silva Costarelli; Gabriele Acuti; Chiara Orlandi; Giovanni Filippini; Elisabetta Chiaradia; Manuela Malatesta; Silvia Crotti; Chiara Antonini; Giulia Amagliani; Elisabetta Manuali; Anna Rita Mastrogiacomo; Livia Moscati; Mohamed Naceur Haouet; Alberto Gaiti; Mauro Magnani

2008-01-01

387

Expression Profile of Genes Representing Varied Spectra of Cell Lineages in Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells  

Microsoft Academic Search

Bone marrow (BM) transplantation is a well-established classical treatment for children with malignant haematologic diseases including leukaemia or fatal metabolic diseases [1]. Mononuclear cells (MNCs) in human BM undergo haemopoiesis into distinct cell lineages such as haemopoietic stem cells and mesenchymal stem cells (MSCs), which are capable of differentiating into varied spectra of cell lineages [2, 3]. Human umbilical cord

Young Joon Moon; Myoung Woo Lee; Mal Sook Yang; Sun Kyung Kim; Joon Seong Park; Hugh C. Kim; Hy-Sook Kim; Kwang-Ho Lee; Young-Jin Kim; Jeongeun Choi

2005-01-01

388

Severe encephalopathy with epilepsy in an infant caused by subclinical maternal pernicious anaemia: case report and review of the literature  

Microsoft Academic Search

Vitamin B 12 deficiency is one of the major causes of megaloblastic anaemia with or without neurological symptoms. We report on a patient manifesting acute encephalopathy, epilepsy, microcephaly and megaloblastic anaemia at the age of 4 months. Vitamin B 12 deficiency in the patient was due to subclinical pernicious anaemia of the mother who exhibited neither haematological nor neurological symptoms.

G. Christoph Korenke; Donald H. Hunneman; Stefan Eber; Folker Hanefeld

2004-01-01

389

Maternal vegan diet causing a serious infantile neurological disorder due to vitamin B 12 deficiency  

Microsoft Academic Search

We present a 9-month-old exclusively breastfed baby of a strict vegetarian mother who had excluded all animal proteins from her diet. The patient's symptoms included dystrophy, weakness, muscular atrophy, loss of tendon reflexes, psychomotor regression and haematological abnormalities. Biochemical investigations revealed severe methylmalonic aciduria and homocystinuria in the patient, slight methylmalonic aciduria in the mother and low concentrations of serum

T. Kiihne; R. Bubl; R. Baumgartner

1991-01-01

390

Bilateral juvenile renal dysplasia in a Norwegian Forest Cat  

Microsoft Academic Search

Renal dysplasia is defined as a condition of disorganised development of renal parenchyma due to abnormal differentiation. The case of a 5-month-old intact male Norwegian Forest Cat with a history of polyuria and polydipsia is reported. Ultrasonographic examination showed a slight enlargement of kidneys. Biochemical parameters, haematological examinations and clinical signs were compatible with chronic renal failure (CRF). Histological examination

Luca Aresu; Renato Zanatta; Paola Pregel; Diego Caliari; Massimiliano Tursi; Federico Valenza; Alberto Tarducci

2009-01-01

391

Frequency and clinical spectrum of rare inherited coagulopathies--a tricenter study  

Microsoft Academic Search

OBJECTIVE: To determine the frequency of rare inherited coagulopathies at three centers of haematology in Karachi and to study the clinical spectrum and laboratory data of these coagulopathies.METHODS: This was a descriptive study conducted from September 2003 to December 2004 on subjects from Aga Khan University Hospital, Husaini Blood Bank and Fatimid Blood Transfusion Centre. All the subjects with bleeding

Safoorah Khalid; Fareena Bilwani; Salman Naseem Adil; Mohammad Khurshid

2008-01-01

392

Audit of start of anticoagulation treatment in inpatients  

Microsoft Academic Search

AIMS: To develop a method for evaluating the start of anticoagulation treatment in inpatients. METHODS: One hundred case notes were audited using a proforma based on local guidelines in accordance with British Society for Haematology recommendations. RESULTS: Confirmatory investigations were done in 93% and 79% of patients with symptomatic deep venous thrombosis and pulmonary embolism, respectively. Identification of patients' risk

G Tan; H Cohen; F Taylor; J Gabbay

1993-01-01

393

Hair zinc level in Down syndrome  

Microsoft Academic Search

Immunological, endocrinological, and haematological abnormalities are relatively common in people with Down syndrome (Cuadrado & Barrena, 1996; Decoq & Vincker, 1995; Hestnes et al., 1991; Sustrova & Strbak, 1994; Nespoli, Burgio, Ugazio & Maccario, 1993; Kempski, Chessells & Reeves, 1997; Kivivuori, Rajantie, & Siimes, 1996; David et al., 1996; Gjertson, Sturm & Berger, 1999). Zinc is one of the elements

Ayse Yenigun; Ferda Ozkinay; Ozgur Cogulu; Canan Coker; Nurten Cetiner; Gonca Ozden; Oguz Aksu; Cihangir Ozkinay

2004-01-01

394

Life-threatening complications of transient abnormal myelopoiesis in neonates with Down syndrome  

Microsoft Academic Search

Neonates with Down syndrome can present with a haematological disorder called transient abnormal myelopoiesis (TAM). While TAM is usually a self-limiting disease, patients with severe complications such as hydrops fetalis, cardiorespiratory failure and liver fibrosis have been described. Here, we present five consecutive neonates with trisomy 21 and TAM, four of whom were critically ill and were therefore treated with

Sabine Dormann; Marcus Krüger; Roland Hentschel; Regina Rasenack; Brigitte Strahm; Udo Kontny; Charlotte Niemeyer

2004-01-01

395

Comparative effects of MS 222 and 2-phenoxyethanol on gilthead sea bream ( Sparus aurata L.) during confinement  

Microsoft Academic Search

The effects of two anaesthetics, ethyl m-aminobenzoate methanosulphonate (MS 222) and 2-phenoxyethanol, at three different dosage levels, were examined on gilthead sea bream (Sparus aurata) during a confinement similar to that in transport. Changes in plasma cortisol, glucose, lactate and haematological parameters were analysed. Confinement without anaesthesia produces a smaller stress response than with the two anaesthetics. Exposure to MS

A. Molinero; J. Gonzalez

1995-01-01

396

Ideal body weight correlates better with engraftment after PBSC autograft than actual body weight, but is under-estimated in myeloma patients possibly due to disease-related height loss  

Microsoft Academic Search

Stem cell dose is important in determining rate of engraftment following autograft. We show closer correlation with haematopoietic reconstitution when the CD34+ cell dose is calculated using ideal (IBW) rather than actual (ABW) body weight in 218 patients receiving peripheral blood stem cell (PBSC) autograft for haematological malignancy. ABW was 21% greater than IBW thus the median CD34+ dose of

P S Maclean; A N Parker; I G McQuaker; A D Clark; E Farrell; K W Douglas

2007-01-01

397

A case of clozapine-induced paralytic ileus  

Microsoft Academic Search

Clozapine is a prototypical second-generation antipsychotic, which has been found to be associated with various adverse effects. Although emphasis has mainly been placed on its haematological and metabolic side effects, less attention has been paid to its anticholinergic effects. One such problem is that of a paralytic ileus. A case of a young man with psychosis who developed a paralytic

Sandeep Rondla; Steven Crane

2007-01-01

398

A case report: Primary amiloidosis associated with immunoglobulin A type multiple myeloma  

Microsoft Academic Search

Primary (AL type) amyloidosis is the most common form of systemic amyloidosis. The morbidity arises from extracellular deposition of immunoglobulin light chain (LC) fibrils in some organs such as the kidneys, heart and bowel. Primary amyloidosis and multiple myeloma both involve clonal plasma cell proliferation. Distinctive haematological and biochemical laboratory findings may help in early diagnosis. Here we present a

Nuri Karadurmus; ?smail Erturk; Mustafa Çakar; Battal Altun; Kenan Saglam; Fikret Arpaci

2011-01-01

399

Multiple myeloma: evolving genetic events and host interactions  

Microsoft Academic Search

Multiple myeloma is a neoplasm of terminally differentiated B cells (plasma cells) in which chromosome translocations frequently place oncogenes under the control of immunoglobulin enhancers. Unlike most haematopoietic cancers, multiple myeloma often has complex chromosomal abnormalities that are reminiscent of epithelial tumours. What causes full-blown myeloma? And can our molecular understanding of this common haematological malignancy be used to develop

W. Michael Kuehl; P. Leif Bergsagel

2002-01-01

400

Hair Zinc Level in Down Syndrome  

ERIC Educational Resources Information Center

|Immunological, endocrinological, and haematological abnormalities are relatively common in people with Down syndrome (Cuadrado & Barrena, 1996; Decoq & Vincker, 1995; Hestnes et al., 1991; Sustrova & Strbak, 1994; Nespoli, Burgio, Ugazio & Maccario, 1993; Kempski, Chessells & Reeves, 1997; Kivivuori, Rajantie, & Siimes, 1996; David et al., 1996;…

Yenigun, Ayse; Ozkinay, Ferda; Cogulu, Ozgur; Coker, Canan; Cetiner, Nurten; Ozden, Gonca; Aksu, Oguz; Ozkinay, Cihangir

2004-01-01

401

INTESTINAL BLOOD FLOW IN SWIMMING CHINOOK SALMON ONCORHYNCHUS TSHAWYTSCHA AND THE EFFECTS OF HAEMATOCRIT ON BLOOD FLOW DISTRIBUTION  

Microsoft Academic Search

Summary Blood flow in the intestinal artery ( qIA), the rate of oxygen consumption (VO•) and a number of haematological variables were measured in chinook salmon, Oncorhynchus tshawytscha, while they swam up to the critical swimming velocity ( Ucrit). The fish used in this study had previously been exposed to one of two different exercise-training regimes, swimming for 8 months

H. THORARENSEN; P. E. GALLAUGHER; A. K. KIESSLING; A. P. FARRELL

1993-01-01

402

A neuropsychotoxicological assessment of workers in a sodium azide production plant  

Microsoft Academic Search

Objective. Despite its known toxicity and extensive current industrial use, the occupational neuropsychotoxicology of sodium azide has not yet been investigated. Method. Neuropsychological and psychological tests, a symptom self-report questionnaire and haematological and cardiac measurements were gathered from 41 exposed workers and 42 unexposed workers in a chemical production plant yearly for 3 years. Results. The exposed workers presented significantly

Sylvain Miljours; Claude M. J. Braun

2003-01-01

403

Theileria ( Babesia ) equi and Babesia caballi Infections in Horses in Galicia, Spain  

Microsoft Academic Search

The control of equine piroplasmosis is becoming increasingly important to maintain the international market open to the horse industry. The purpose of this study was to demonstrate the occurrence of equine piroplasmosis (Theileria equi and Babesia caballi) in Galicia, north-west Spain, and to compare haematological and serum biochemistry parameters between non-parasitaemic horses and horses parasitaemic with T. equi and B.

A. T. Camacho; F. J. Guitian; E. Pallas; J. J. Gestal; A. S. Olmeda; M. A. Habela; S. R. Telford III; A. Spielman

2005-01-01

404

Pyoderma gangrenosum after totally implanted central venous access device insertion  

Microsoft Academic Search

BACKGROUND: Pyoderma gangrenosum is an aseptic skin disease. The ulcerative form of pyoderma gangrenosum is characterized by a rapidly progressing painful irregular and undermined bordered necrotic ulcer. The aetiology of pyoderma gangrenosum remains unclear. In about 70% of cases, it is associated with a systemic disorder, most often inflammatory bowel disease, haematological disease or arthritis. In 25–50% of cases, a

Ihsan Inan; Patrick O Myers; Rolf Braun; Monica E Hagen; Philippe Morel

2008-01-01

405

Nutritional vitamin B 12 deficiency: Two cases detected by routine newborn urinary screening  

Microsoft Academic Search

We describe two asymptomatic newborns with nutritional vitamin B12 deficiency in whom increased urinary methylmalonic acid was detected by routine neonatal screening at 3 weeks of age. Both infants were exclusively breast-fed. One mother suffered from pernicious anaemia, and the other was a strict vegetarian. Both mothers had no clinical or haematological abnormality, aside from a borderline mean corpuscular volume

J. L. Michaud; B. Lemieux; H. Ogier; M. A. Lambert

1992-01-01

406

The effect of short term feeding with organic and conventional diets on selected immune parameters in rat  

Microsoft Academic Search

There is currently no evidence for beneficial health impacts being associated with the consumption of organic rather than conventional foods. This preliminary study was therefore aimed at using haematological parameters, white blood cell (WBC) number and splenocyte proliferation as sensitive assays to evaluate influence of the organic, low input and conventional components in the diet on rats' immune system function.

K. Brandt; L. Lueck; C. Leifert

407

Molecular heterogeneity of breast carcinomas and the cancer stem cell hypothesis  

Microsoft Academic Search

Human breast cancers are heterogeneous, both in their pathology and in their molecular profiles. This suggests the hypothesis that breast cancers can initiate in different cell types, either breast epithelial stem cells or their progeny (transit amplifying cells or committed differentiated cells). In this respect, breast cancer could be viewed as being similar to haematological malignancies for which an analogous

John Stingl; Carlos Caldas

2007-01-01

408

New pharmacokinetic and pharmacodynamic tools for interferon-alpha (IFN-a) treatment of human cancer  

Microsoft Academic Search

Interferon ? (IFN-?) has been widely used in the treatment of human solid and haematologic malignancies. Although the antitumour activity of IFN-? is well recognised at present, no major advances have been achieved in the last few years. Recent findings have provided new information on the molecular mechanisms of the antitumour activity of the cytokine. In fact, IFN-? appears to

Pierosandro Tagliaferri; Michele Caraglia; Alfredo Budillon; Monica Marra; Giovanni Vitale; Caterina Viscomi; Serena Masciari; Pierfrancesco Tassone; Alberto Abbruzzese; Salvatore Venuta

2005-01-01

409

Sensitive PCR method for the detection and real-time quantification of human cells in xenotransplantation systems  

Microsoft Academic Search

The sensitive detection of human cells in immunodeficient rodents is a prerequisite for the monitoring of micrometastasis of solid tumours, dissemination of leukaemic cells, or engraftment of haematological cells. We developed a universally applicable polymerase chain reaction method for the detection of a human-specific 850-bp fragment of the ?-satellite DNA on human chromosome 17. The method allows the detection of

M Becker; A Nitsche; C Neumann; J Aumann; I Junghahn; I Fichtner

2002-01-01

410

Role of nucleophosmin in embryonic development and tumorigenesis  

Microsoft Academic Search

Nucleophosmin (also known as NPM, B23, NO38) is a nucleolar protein directly implicated in cancer pathogenesis, as the NPM1 gene is found mutated and rearranged in a number of haematological disorders. Furthermore, the region of chromosome 5 to which NPM1 maps is deleted in a proportion of de novo human myelodysplastic syndromes (MDS), and loss of chromosome 5 is extremely

Silvia Grisendi; Rosa Bernardi; Marco Rossi; Ke Cheng; Luipa Khandker; Katia Manova; Pier Paolo Pandolfi

2005-01-01

411

Lymphoma and Myeloma Cell Resistance to Cytotoxic Agents and Ionizing Radiations Is Not Affected by Exposure to Anti–IL6 Antibody  

Microsoft Academic Search

Background: Production of high levels of IL-6 is often correlated with resistance to cytotoxics or ionizing radiations, in cancer cell lines as in various cancer patients. We investigated whether monoclonal antibodies directed against IL-6 may enable to reverse resistance of cancer cell lines. Methodology\\/Principal Findings: We exposed ten haematological cancer cells from lymphoma, myeloma, or leukemia origins to cytotoxics or

Angélique Gougelet; Adeline Mansuy; Jean-Yves Blay; Laurent Alberti; Claudine Vermot-Desroches

2009-01-01

412

Adult stem cell therapy beyond haemopoietic stem cell transplantation? An update  

Microsoft Academic Search

The lifesaving potential of haemopoietic stem cell transplantation for the treatment of haematological malignancies and other life threatening disorders of the haemopoietic stem cell is universally accepted. In contrast, the use of adult marrow derived stem cells for tissue repair strategies in degenerative disease or after tissue damage are only in the early stages of evolution. A range of opinion

Jill Hows

2005-01-01

413

CsA exposure is associated with acute GVHD and relapse in children after SCT  

Microsoft Academic Search

CsA is commonly used after haematological SCT (HSCT) as GVHD prophylaxis. In solid organ transplantation, area under the blood concentration vs time curve (AUC) correlates with clinical outcome. However, in HSCT, it has not been determined whether the AUC is superior to trough level monitoring to optimize clinical efficacy of CsA therapy. Therefore, the aim of this study was to

A J Willemze; A C Lankester; R M Egeler; J den Hartigh; J M Vossen

2010-01-01

414

Therapeutic implications of recombinant human erythropoietin in anaemic related clinical manifestations  

Microsoft Academic Search

The introduction of recombinant human erythropoietin (RHUEPO) has revolutionised the treatment strategies for patients suffering with anaemia of chronic renal disease and chronic heart failure. Clinical studies and several observational evidences have demonstrated that RHUEPO is also useful in various non-uraemic conditions including haematological and oncological disorders, prematurity, HIV infection and preoperative therapies. The successful treatment of all the anaemic

D. Annieruth Helena; A. Jyothsna; N. Lakshmi; G. Venkateswara Rao; K. R. S. Sambasiva Rao

2006-01-01

415

The No-Effect Level of Sodium Bromide in Healthy Volunteers  

Microsoft Academic Search

1 A total of 0, 4 and 9 mg kg-1 body weight sodium bromide was administered orally to 45 healthy female volunteers.2 The experiment lasted for six menstrual cycles: only during the first three cycles was bromide administered;3 At the start, at the end of the administration period and at the end of the experiment a physical examination and haematological

C. E. M. van Gelderen; T. J. F. Savelkoul; J. L. Blom; W. van Dokkum; R. Kroes

1993-01-01

416

Automated measurement of schistocytes after bone marrow transplantation  

Microsoft Academic Search

Bone marrow transplantation-related thrombotic microangiopathy (BMT-TMA) is a severe complication partly suspected on the evidence of a microangiopathic haemolysis. Microscopic schistocyte observation confirms the mechanical origin of the haemolysis, but remains a tedious procedure that lacks standardization. Direct measurement of abnormal red blood cell (RBC) fragments is now available on some automated haematology systems. We compared in 131 patients (69

J-F Lesesve; S Salignac; T Lecompte; P Bordigoni

2004-01-01

417

The burden of the ‘RA RA’ positive: survivors’ and hospice patients’ reflections on maintaining a positive attitude to serious illness  

Microsoft Academic Search

Goals This article seeks to make a contribution to fostering work on the neglected area of research about how individuals maintain a positive outlook during their confrontation with serious illness. Insights are presented from both haematology survivors and hospice patients about the factors that hinder and facilitate their capacity to ‘be positive’. Patients and methods Open-ended interviews with 14 hospice

Pam McGrath

2004-01-01

418

Computer-Analysen der Erythrocytenvolumenverteilungskurven  

Microsoft Academic Search

Summary The erythrocyte diameter curve of Price-Jones exhibits symmetrical, normal distribution. In contrast, the volume distribution curve of erythrocytes shows an asymmetrical course with some skewness to the right, which with different haematological diseases may vary. With respect to differential diagnosis as well as to therapy it is of importance to have available an objective means of comparing curves with

A. Schäfer; W. Schäfer

1980-01-01

419

Deprivation of Drinking Water for up to 48 Hours Does Not Affect the Osmotic Fragility of Erythrocytes from Captive Helmeted Guinea Fowl (Numida meleagris)  

Microsoft Academic Search

Poor management practices may result in poultry having inadequate drinking water for prolonged periods and consequently adverse haematological effects. The effects of water deprivation for up to 48 hours on osmotic fragility of erythrocytes, plasma osmolarity and erythrocyte indices was investigated in eighteen, female Guinea fowl. Six birds had drinking water ad libitum and the other 12 were deprived of

B. Mafuvadze; M. Nyanungo; H. Saina; B. Gorejena; T. Mashayamombe; K. H. Erlwanger

2008-01-01

420

Antiangiogenic activity of aplidine, a new agent of marine origin  

Microsoft Academic Search

The antineoplastic compound aplidine, a new marine-derived depsipeptide, has shown preclinical activity in vitro on haematological and solid tumour cell lines. It is currently in early phase clinical trials. The exact mechanism of action of this anticancer agent still needs to be clarified. We have previously reported that aplidine blocks the secretion of the angiogenic factor vascular endothelial growth factor

G Taraboletti; M Poli; R Dossi; L Manenti; P Borsotti; G T Faircloth; M Broggini; M D’Incalci; D Ribatti; R Giavazzi

2004-01-01

421

Risk assessment in Nile tilapia (Oreochromis niloticus) and African mud catfish (Clarias gariepinus) exposed to cassava effluent  

Microsoft Academic Search

Toxicity of cassava effluent in water on aquatic lives was examined via acute and chronic bioassay experiments on Clarias gariepinus (I) and Oreochromis niloticus (II) under laboratory conditions, using standard procedures. The effluent cyanide concentration exceeded the WHO limit for wastewater. Fish body weights and haematological parameters (HMP) significantly decreased with increasing effluent concentration at p?0.05. Toxicity on HMP for

I. M. Adekunle; T. A. Arowolo; I. T. Omoniyi; O. T. Olubambi

2007-01-01

422

Neutrophils and immunity: challenges and opportunities  

Microsoft Academic Search

Scientists who study neutrophils often have backgrounds in cell biology, biochemistry, haematology, rheumatology or infectious disease. Paradoxically, immunologists seem to have a harder time incorporating these host-defence cells into the framework of their discipline. The recent literature discussed here indicates that it is appropriate for immunologists to take as much interest in neutrophils as in their lymphohaematopoietic cousins with smooth

Carl Nathan

2006-01-01

423

[Radioecological problems of aquatic ecosystems of the Chernobyl exclusion zone].  

PubMed

The results of radioactive contamination dynamics in the main components of aquatic ecosystems and the absorbed dose rate for hydrobionts within the Chernobyl accident exclusion zone was analysed. Some cytogenetical and haematological effects of long-term irradiation on aquatic organisms as well as damage of higher aquatic plants by parasitic fungi and gall-producing arthropods were considered. PMID:19507688

Gudkov, D I; Kuz'menko, M I; Kireev, S I; Nazarov, A B; Shevtsova, N L; Dziubenko, E V; Kaglian, A E

424

West Syndrome in an Infant with Vitamin B[subscript 12] Deficiency in the Absence of Macrocytic Anaemia  

ERIC Educational Resources Information Center

|Vitamin B[subscript 12] deficiency in infants often produces haematological and neurological deficits, including macrocytic anaemia, neurodevelopmental delay or regression, irritability, weakness, hypotonia, ataxia, apathy, tremor, and seizures. The diagnosis of vitamin B[subscript 12] deficiency can be difficult when the typical macrocytic…

Erol, Ilknur; Alehan, Fusun; Gumus, Ayten

2007-01-01

425

Increased blood oxygen affinity during digestion in the snake Python molurus  

Microsoft Academic Search

Many snakes exhibit large increases in metabolic rate during digestion that place extensive demands on efficient oxygen transport. In the present study, we describe blood oxygen affinity following three weeks of fasting and 48 h after feeding in the Burmese python (Python molurus). We also report simultaneous measurements of arterial blood gases and haematological parameters. Arterial blood was obtained from

Johannes Overgaard; Tobias Wang

426

Monocyte–macrophage system as targets for immunomodulation by intravenous immunoglobulin  

Microsoft Academic Search

Pooled human intravenous immunoglobulin (IVIg) has been used successfully to treat or ameliorate the clinical manifestations of humoral immune deficiencies, haematological disorders, HIV infection and many other diseases states. However, the mechanism of action of IVIg remains unclear. Several mechanisms of action of IVIg have been proposed. These include Fc? receptor blockade, accelerated clearance of endogenous pathogenic auto-antibodies, inhibition of

C. J. Rhoades; M. A. Williams; S. M. Kelsey; A. C. Newland

2000-01-01

427

Quality of life and factors related to perceived satisfaction with quality of life after allogeneic bone marrow transplantation  

Microsoft Academic Search

The quality of life after bone marrow transplantation (BMT) was studied in 109 adult allogeneic BMT recipients transplanted on at the Helsinki University Central hospital for a haematological malignancy. Physical, functional, emotional and social well-being was measured on the Functional Assessment of Cancer Therapy Scale (FACT-BMT) and a shortened version of the Profile of Mood States Scale (POMS) and by

Heikki Heinonen; Liisa Volin; Antti Uutela; Michael Zevon; Chris Barrick; Tapani Ruutu

2001-01-01

428

Dengue and other Febrile Illnesses among Children in the Philippines  

Microsoft Academic Search

In view of the increasing trend of DHF in the Philippines, a prospective study was undertaken to define the clinical and haematological differences between DF\\/DHF and other febrile illnesses (OFI) between 1999 and 2001. A total of 503 paediatric patients, who had acute febrile illness without an apparent focus of infection and who were admitted to St. Luke's Medical Center

Kazunori Oishia; Cynthia A. Mapuae; Celia C. Carlosd; Maria T. D. D. Cinco-Abanesd; Mariko Saitob; Shingo Inouec; Kouichi Moritac; Filipinas F. Natividade

429

[Reactivation of hepatitis B associated with immunosuppression].  

PubMed

The hepatitis B virus is an important cause of viral reactivation and flares in immunosuppressed patients. Factors associated with viral reactivation include positive HBs antigen, rituximab treatment, onco-haematological pathology and bone marrow transplantation. In situations at high risk of viral reactivation prophylactic antiviral therapy is indicated and reduces morbidity and mortality related to viral hepatitis flares. PMID:24066463

Goossens, N; Negro, F

2013-09-01

430

Nodular regenerative hyperplasia of the liver, CREST syndrome and primary biliary cirrhosis: an overlap syndrome?  

Microsoft Academic Search

Nodular regenerative hyperplasia of the liver (NRHL) has been found in association with collagen vascular diseases, after drug therapy, with autoimmune disease, and with a variety of haematological disorders. The association of NRHL with the syndrome of Calcinosis cutis, Raynaud's phenomenon, oesophageal dysfunction, sclerodactyly and telangiectasia (CREST syndrome) has only been reported on two previous occasions. The liver disease usually

R F McMahon; C Babbs; T W Warnes

1989-01-01

431

Ensuring accurate oral mucositis assessment in the European Group for Blood and Marrow Transplantation Prospective Oral Mucositis Audit  

Microsoft Academic Search

Oral mucositis (OM) has substantial negative clinical, quality-of-life, and economic consequences for patients with haematologic malignancies who require myeloablative chemotherapy or radiotherapy. Uniform training in OM assessment is infrequent in clinical practice, so the true incidence and duration of OM are unknown. Nurses and physicians from the European Group for Blood and Marrow Transplantation recently undertook an audit of 214

Barry Quinn; Rebecca Stone; Mary Uhlenhopp; Shaun McCann; Nicole Blijlevens

2007-01-01

432

Effect of iron supplementation on mild to moderate anaemia in pulmonary tuberculosis  

Microsoft Academic Search

Anaemia is a common complication of pulmonary tuberculosis. The precise mechanism of anaemia in pulmonary tuberculosis is not clearly known, but anaemia of inflammation as well as of Fe deficiency has been implicated. Both are common in developing countries. It is extremely difficult to distinguish anaemia of Fe deficiency from anaemia of inflammation with the haematological indices used rou- tinely.

Uma Devi; C. Mohan Rao; Vinod K. Srivastava; Pramod K. Rath; Bhabani S. Das

2003-01-01

433

Amegakaryocytic thrombocytopenia and immune-mediated haemolytic anaemia in a cat  

Microsoft Academic Search

An eleven-month-old female spayed domestic shorthair cat was presented for investigation of pruritic skin lesions and haemorrhage. Haematological evaluation revealed a markedly regenerative anaemia, neutrophilia with left shift, monocytosis, basophilia and severe thrombocytopenia. The direct antiglobulin test and serum antinuclear antibody tests were positive. Serologic and immunofluorescent antibody tests for feline leukaemia virus were negative. Bone marrow cytology revealed a

F. P. Gaschenl; B. Smith Meyer; John W. Harvey

1992-01-01

434

Paclitaxel, ifosfamide and cisplatin with granulocyte colony-stimulating factor or recombinant human interleukin 3 and granulocyte colony-stimulating factor in ovarian cancer: a feasibility study.  

PubMed Central

The tolerability and efficacy of four courses of paclitaxel and ifosfamide plus cisplatin every 3 weeks was evaluated in patients with residual or refractory ovarian cancer. Additionally, supportive haematological effects of recombinant human interleukin 3 (rhIL-3) and recombinant human granulocyte colony-stimulating factor (G-CSF) were studied. Paclitaxel starting dose was 135 mg m(-2) (day 1), with ifosfamide dose 1.2 g m(-2) day(-1) (days 2-4) and cisplatin dose 30 mg m(-2) day(-1) (days 2-4). All 16 patients received 5.0 microg kg(-1) day(-1) G-CSF (days 7-16) and, in addition, eight patients were randomized to receive 10 microg kg(-1) day(-1) rhIL-3 (days 5-9). Paclitaxel and ifosfamide doses were reduced when grade IV haematological toxicity occurred. In the absence of grade IV haematological toxicity and normal recovery of haematopoiesis, paclitaxel dose was escalated. Toxicity was evaluable in 56 courses, with haematological effects in 52. Despite antiemetic treatment, nausea and vomiting (> or = grade I) occurred in 50 courses. Five patients had persisting peripheral neuropathy. Renal and liver function were not affected. Grade IV neutropenia occurred in 12 out of 52 courses, with neutropenic fever in two patients, both of whom died from fatal septicaemia. Grade IV thrombocytopenia without bleeding was observed in 15 courses. Grade IV haematological toxicity was associated with hepatic metastases and concurrent increases in alkaline phosphatase (P <0.001) and gamma-glutamyltransferase (P=0.007). No relation was found between haematological toxicity and pharmacokinetic parameters of paclitaxel. Patients treated with rhIL-3 showed a tendency to a faster platelet recovery (not affecting platelet nadir), and the cisplatin dose intensity was higher (P=0.025). Six of the nine evaluable patients had a tumour response. The overall median progression-free survival was 7 months and the overall mean survival was 13 months. In conclusion, this potentially interesting combination as second-line treatment showed a low tolerability with unexpected mortality, while rhIL-3 administration tended to induce a more rapid platelet recovery.

Veldhuis, G. J.; Willemse, P. H.; Beijnen, J. H.; Boonstra, H.; Piersma, H.; van der Graaf, W. T.; de Vries, E. G.

1997-01-01

435

Pathophysiology of white-nose syndrome in bats: a mechanistic model linking wing damage to mortality.  

PubMed

White-nose syndrome is devastating North American bat populations but we lack basic information on disease mechanisms. Altered blood physiology owing to epidermal invasion by the fungal pathogen Geomyces destructans (Gd) has been hypothesized as a cause of disrupted torpor patterns of affected hibernating bats, leading to mortality. Here, we present data on blood electrolyte concentration, haematology and acid-base balance of hibernating little brown bats, Myotis lucifugus, following experimental inoculation with Gd. Compared with controls, infected bats showed electrolyte depletion (i.e. lower plasma sodium), changes in haematology (i.e. increased haematocrit and decreased glucose) and disrupted acid-base balance (i.e. lower CO2 partial pressure and bicarbonate). These findings indicate hypotonic dehydration, hypovolaemia and metabolic acidosis. We propose a mechanistic model linking tissue damage to altered homeostasis and morbidity/mortality. PMID:23720520

Warnecke, Lisa; Turner, James M; Bollinger, Trent K; Misra, Vikram; Cryan, Paul M; Blehert, David S; Wibbelt, Gudrun; Willis, Craig K R

2013-05-29

436

Rare association of Visceral leishmaniasis with Hodgkin's disease: A case report.  

PubMed

We present here a case of young male with complaints of fever and swelling in the neck for eight months. History of progressive weakness associated with weight loss was present. Physical examination revealed pallor, multiple enlarged cervical lymph nodes and hepatosplenomegaly. Investigations showed pancytopenia, hyperglobinemia and Leishman-Donovan bodies on bone marrow aspiration. Serological test confirmed diagnosis of visceral leishmaniasis. However, cervical lymph node aspiration and biopsy were suggestive of Mixed cellularity Hodgkin's disease. This made it a very rare case of Leishmaniasis as an opportunistic infection in a patient of pre-chemotherapy Hodgkin's disease. There was marked improvement in haematological profile and regression of hepatosplenomegaly with Amphotericin B treatment followed by favourable response to chemotherapy. The case emphasizes the suspicion for leishmaniasis as a masquerader and as an opportunistic infection in haematological malignancies. PMID:22011565

Kumar, Rakshit; Daga, Mradul K; Kamble, Nitish L; Sothwal, Arpit; Singh, Tejinder; Nayak, Hemanta K; Raizada, Nishant

2011-10-20

437

Smoking--a major cause of polycythaemia.  

PubMed Central

The importance of smoking in the aetiology of polycythaemia has been assessed in a group of patients referred to a general haematology clinic. All patients with true and relative polycythaemia (excluding those with polycythaemia rubra vera) were studied. Of the 21 such patients evaluated, 14 were smokers and had raised carboxyhaemoglobin levels and had no other demonstrable cause for their polycythaemia. The commonest physiological abnormality in these patients was a raised red cell mass combined with a low plasma volume. Six of the 14 patients were able to reduce their smoking with subsequent improvement in their haematocrits. These results suggest that smoking is a major cause of polycythaemia in an unselected series of referrals to a general haematology clinic. The early identification of these patients may be useful in planning therapy.

Aitchison, R; Russell, N

1988-01-01

438

Pathophysiology of white-nose syndrome in bats: a mechanistic model linking wing damage to mortality  

USGS Publications Warehouse

White-nose syndrome is devastating North American bat populations but we lack basic information on disease mechanisms. Altered blood physiology owing to epidermal invasion by the fungal pathogen Geomyces destructans (Gd) has been hypothesized as a cause of disrupted torpor patterns of affected hibernating bats, leading to mortality. Here, we present data on blood electrolyte concentration, haematology and acid–base balance of hibernating little brown bats, Myotis lucifugus, following experimental inoculation with Gd. Compared with controls, infected bats showed electrolyte depletion (i.e. lower plasma sodium), changes in haematology (i.e. increased haematocrit and decreased glucose) and disrupted acid–base balance (i.e. lower CO2 partial pressure and bicarbonate). These findings indicate hypotonic dehydration, hypovolaemia and metabolic acidosis. We propose a mechanistic model linking tissue damage to altered homeostasis and morbidity/mortality.

Warnecke, Lisa; Turner, James M.; Bollinger, Trent K.; Misra, Vikram; Cryan, Paul M.; Blehert, David S.; Wibbelt, Gudrun; Willis, Craig K. R.

2013-01-01

439

Rare association of Visceral leishmaniasis with Hodgkin's disease: A case report  

PubMed Central

We present here a case of young male with complaints of fever and swelling in the neck for eight months. History of progressive weakness associated with weight loss was present. Physical examination revealed pallor, multiple enlarged cervical lymph nodes and hepatosplenomegaly. Investigations showed pancytopenia, hyperglobinemia and Leishman-Donovan bodies on bone marrow aspiration. Serological test confirmed diagnosis of visceral leishmaniasis. However, cervical lymph node aspiration and biopsy were suggestive of Mixed cellularity Hodgkin's disease. This made it a very rare case of Leishmaniasis as an opportunistic infection in a patient of pre-chemotherapy Hodgkin's disease. There was marked improvement in haematological profile and regression of hepatosplenomegaly with Amphotericin B treatment followed by favourable response to chemotherapy. The case emphasizes the suspicion for leishmaniasis as a masquerader and as an opportunistic infection in haematological malignancies.

2011-01-01

440

Antitumour effect of Diospyros cordifolia bark on Ehrlich ascites carcinoma-bearing Swiss albino mice.  

PubMed

Diospyros cordifolia Roxb. (Ebenaceae), commonly known as Indian ebony, is used traditionally for several medicinal purposes. In this study, the methanol extract of D. cordifolia bark (MEDC) was evaluated for its antitumour effect against Ehrlich ascites carcinoma (EAC)-bearing Swiss albino mice. Twenty-four hours after intraperitoneal inoculation of tumour (EAC) cells in mice, MEDC was administered intraperitoneally at 25 and 50?mg kg?ą bodyweight for 9 consecutive days. On the 10th day, half of the mice were sacrificed to determine the tumour volume, viable and non-viable tumour cell counts, and rest were kept alive for the assessment of median survival time and increase in life span. Haematological profiles were also determined. MEDC exhibited a marked decrease in tumour growth parameters and increased the survival rate of EAC-bearing animals. MEDC normalised the haematological parameters as compared with the EAC control mice. Therefore, this study demonstrated that D. cordifolia bark possessed remarkable antitumour efficacy. PMID:21985607

Das, Sudipta; Bhattacharya, Sanjib; Pramanik, Goutam; Haldar, Pallab Kanti

2011-10-10

441

Benchmark dose approach for low-level lead induced haematogenesis inhibition and associations of childhood intelligences with ALAD activity and ALA levels.  

PubMed

Lead (Pb) levels, delta-aminolevulinic acid dehydratase (ALAD) activities, zinc protoporphyrin (ZPP) levels in blood, and urinary delta-aminolevulinic acid (ALA) and coproporphyrin (CP) concentrations were measured for 318 environmental Pb exposed children recruited from an area of southeast China. The mean of blood lead (PbB) levels was 75.0?g/L among all subjects. Benchmark dose (BMD) method was conducted to present a lower PbB BMD (lower bound of BMD) of 32.4?g/L (22.7) based on ALAD activity than those based on the other three haematological indices, corresponding to a benchmark response of 1%. Childhood intelligence degrees were not associated significantly with ALAD activities or ALA levels. It was concluded that blood ALAD activity is a sensitive indicator of early haematological damage due to low-level Pb exposures for children. PMID:21334730

Wang, Q; Ye, L X; Zhao, H H; Chen, J W; Zhou, Y K

2011-02-21

442

Use of Hematopoietic Growth Factor in the Management of Hematological Side Effects Associated to Antiviral Treatment for Hcv Hepatitis  

PubMed Central

Haematological abnormalities are common during combination antiviral therapy for chronic hepatitis C. Although dose reduction or discontinuation can easily treat these side effects, they can adversely affect the efficacy of combination antiviral therapy reducing the likelihood of a sustained viral response (SVR). To avoid potentially diminishing a patient’s chance of response, many physicians have begun using growth factors off-label to manage anaemia and neutropenia in hepatitis C. Haematopoietic growth factors are generally well tolerated and they may be useful for managing haematological side effects of anti-HCV therapy improving patients’ quality of life. To date, the role and benefit of these agents during anti-HCV therapy and their positive impact on SVR have not conclusively determined in the published studies. However, the possibility of a benefit to individual outpatients remains, and an individualized approach is recommended. This review explores the incidence, clinical significance, and management of anaemia, neutropenia and thrombocytopenia associated with combination therapy for HCV infection.

Mancino, Paola; Falasca, Katia; Ucciferri, Claudio; Pizzigallo, Eligio; Vecchiet, Jacopo

2010-01-01

443

Persistence of neurological damage induced by dietary vitamin B-12 deficiency in infancy  

PubMed Central

Accepted 9 April 1997? A case is reported of a 14 month old boy with severe dietary vitamin B-12 deficiency caused by his mother's vegan diet. Cinical, electroencephalography (EEG), and haematological findings are described. Cranial magnetic resonance imaging (MRI) showed severe frontal and frontoparietal cranial atrophy. Vitamin B-12 supplements led to a rapid improvement of haematological and neurological symptoms. Serum vitamin B-12 and urinary methylmalonate excretion were normal 10 days after treatment began. After six weeks, EEG was normal and cranial MRI after 10 weeks showed complete disappearance of all structural abnormalities. Cognitive and language development, however, remained seriously retarded at the age of 2 years. It is concluded that infantile vitamin B-12 deficiency induced by maternal vegan diets may cause lasting neurodisability even though vitamin B-12 supplementation leads to rapid resolution of cerebral atrophy and electroencephalographic abnormality.??

von Schenck, U.; Bender-Gotze, C.; Koletzko, B.

1997-01-01

444

Catheter-related thrombosis in cancer patients.  

PubMed

Central venous catheters are commonly used in haematology departments for the administration of chemotherapy, blood products and parenteral nutrition in patients with haematological malignancy. Thrombosis is a recognized complication of such devices especially in oncology patients. Catheter-related thrombi (CRT) may lead to pulmonary embolism and infection, as well as catheter failure and potential delays in treatment. The vast majority of CRT are asymptomatic, thus a high index of suspicion is required in making the diagnosis. Doppler ultrasound or venography may be employed to identify CRT. Once confirmed, the initiation of treatment is a balance between the risks of anticoagulation against potential complications of CRT. A number of risk factors for CRT are discussed along with an overview of catheter types, their appropriate choice and intravascular positioning. PMID:23848991

Murray, Jim; Precious, Elizabeth; Alikhan, Raza

2013-07-15

445

Moving cages further offshore: effects on southern bluefin tuna, T. maccoyii, parasites, health and performance.  

PubMed

The effects of offshore aquaculture on SBT health (particularly parasitic infections and haematology) and performance were the main aim of this study. Two cohorts of ranched Southern Bluefin tuna (SBT) (Thunnus maccoyii) were monitored throughout the commercial season, one maintained in the traditional near shore tuna farming zone and one maintained further offshore. SBT maintained offshore had reduced mortality, increased condition index at week 6 post transfer, reduced blood fluke and sealice loads, and haematological variables such as haemoglobin or lysozyme equal to or exceeding near shore maintained fish. The offshore cohort had no Cardicola forsteri and a 5% prevalence of Caligus spp., compared to a prevalence of 85% for Cardicola forsteri and 55% prevalence for Caligus spp. near shore at 6 weeks post transfer. This study is the first of its kind to examine the effects of commercial offshore sites on farmed fish parasites, health and performance. PMID:21901129

Kirchhoff, Nicole T; Rough, Kirsty M; Nowak, Barbara F

2011-08-25

446

Outcome of match related allogeneic stem cell transplantation procedures performed from 2004 till 2011  

PubMed Central

We present our initial experience of allogeneic stem cell transplant procedure performed between April 2004 and August 2011 for various haematological disorders. All patients with non-malignant and malignant haematological disorders with HLA matched donors were selected after pre-transplant workup. Ninety seven patients underwent the procedure. Most common indications for transplant were aplastic anaemia in n?=?34 (35%), followed by ?-Thalassemia major in n?=?21 (21.6%) and chronic myeloid leukemia in n?=?11 patients (11.3%). Primary graft failure present was present in 2.06%. Incidence of graft versus host disease (GvHD) in our patients was 34%. After median follow-up of five years the overall survival was 71.3% with a mean survival time of 51.2?±?3.3?months.

2012-01-01

447

Long-term air pollution exposure and risk factors for cardiovascular diseases among the elderly in Taiwan  

Microsoft Academic Search

ObjectivesTo investigate changes in blood pressure, blood lipids, blood sugar and haematological markers of inflammation associated with changes in long-term exposure to ambient air pollutants.MethodsWe conducted secondary analyses of data on blood pressure and blood biochemistry markers from the Social Environment and Biomarkers of Aging Study in Taiwan and air pollution data from the Taiwan Environmental Protection Administration in 2000.

Kai-Jen Chuang; Yuan-Horng Yan; Shu-Yi Chiu; Tsun-Jen Cheng

2010-01-01

448

Amphotericin B colloidal dispersion (Amphocil) vs fluconazole for the prevention of fungal infections in neutropenic patients: data of a prematurely stopped clinical trial  

Microsoft Academic Search

We conducted an open label, randomised clinical trial to compare amphotericin B colloidal dispersion (ABCD, Amphocil) 2 mg\\/kg\\/day intravenously with fluconazole 200 mg\\/day orally, for the prevention of fungal disease in neutropenic patients with haematological malignancies. In the event of unresolved fever after 4 days of empirical antibacterial therapy, patients in both treatment groups were to receive ABCD, 4 mg\\/kg\\/day.

GJ Timmers; S Zweegman; AM Simoons-Smit; AC van Loenen; D Touw; PC Huijgens

2000-01-01

449

Osmoregulation in juvenile Siberian sturgeon ( Acipenser baerii )  

Microsoft Academic Search

The osmoregulation capabilities of juvenile Siberian sturgeon exposed to three experimental osmolalities (22, 250 and 387 mOsmol kg?1) were studied over a 45-day period. Growth performance, haematological parameters, ion concentrations, gill and spiral valve\\u000a Na+-K+-ATPase activities, as well as gill and spiral valve histology, were measured. At the end of the period, the plasma osmolality\\u000a of fish kept in 250 and 387 mOsmol kg?1

A. Rodríguez; M. A. Gallardo; E. Gisbert; S. Santilari; A. Ibarz; J. Sánchez; F. Castelló-Orvay

2002-01-01

450

Efficacy of Amodiaquine in uncomplicated falciparum malaria in Nigeria in an area with high-level resistance to Chloroquine and Sulphadoxine\\/Pyrimethamine  

Microsoft Academic Search

Falciparum Malaria is hyperendemic in southern Nigeria and chloroquine resistance is an increasing problem. Therefore, the parasitological and haematological response to treatment with amodiaquine was studied in children under 5 years during a 14-day follow-up. Of 105 children who accomplished the study (out of 114 who were enrolled), 95.3% were parasite-negative on thick blood film on day 7, which decreased to

Jens Graupner; Klaus Göbels; Martin P. Grobusch; Anne Lund; Joachim Richter; Dieter Häussinger

2005-01-01

451

Anaemia associated with vitamin D deficiency rickets  

Microsoft Academic Search

AimTo elucidate the relation between vitamin D deficiency rickets and anaemia.MethodsInfants with rickets or hypocalcaemia seen in a 200 bed children's hospital were haematologically studied. All cases were divided into active or healed rickets according to radiological evidence.ResultsThe Hb ranged from 5 to 14 g\\/l and 48% of the cases of active rickets were considered in the range of anaemia,

A T H Elidrissy; M A Zolali; Z Hawsawi

2011-01-01

452

Effects of Epidural Ketamine–Xylazine Combination on the Clinicophysiological and Haematobiochemical Parameters of Uraemic and Healthy Goats  

Microsoft Academic Search

Xylazine–ketamine combination was evaluated for its efficacy and safety after epidural administration in uraemic and healthy\\u000a goats. The combination (xylazine 0.025 mg\\/kg and ketamine 2.5 mg\\/kg) was administered to uraemic (n = 6) and healthy (n = 6) animals in the lumbosacral epidural space. The combination was evaluated in terms of clinical, physiological, haematological\\u000a and biochemical parameters. The onset of

K. Singh; P. Kinjavdekar; Amarpal; H. P. Aithal; A. Gopinathan; G. R. Singh; A. M. Pawde; K. Pratap

2007-01-01

453

Epidemiology of Invasive Pulmonary Aspergillosis  

Microsoft Academic Search

\\u000a The epidemiology of IA, the major invasive mould infection in immunocompromised patients, has evolved over the last several\\u000a decades. During the 1990s, increasing morbidity and mortality from these infections, particularly amongst the increasing numbers\\u000a of patients being treated for haematological malignancies and those undergoing allogeneic haematopoietic stem cell transplantation,\\u000a became a universal experience in many tertiary care medical centres. Changes

Carol A. Kauffman; Nelson P. Nicolasora

454

Partial splenectomy in cystic fibrosis patients with hypersplenism  

PubMed Central

We report three cystic fibrosis (CF) patients with hypersplenism who underwent partial splenectomy. The postoperative course was uneventful in two patients; one patient developed a complication necessitating resection of the rest of the spleen. Haematological parameters improved and oesophageal varices regressed in all patients. On follow up, one patient showed a normal spleen, the other a normally functioning accessory spleen; the third patient again developed splenomegaly with hypersplenism. Partial splenectomy is a promising therapeutic option for CF patients with hypersplenism.

Thalhammer, G; Eber, E; Uranus, S; Pfeifer, J; Zach, M; Kelly, D; de Ville, de Goyet J

2003-01-01

455

Partial splenectomy in cystic fibrosis patients with hypersplenism.  

PubMed

We report three cystic fibrosis (CF) patients with hypersplenism who underwent partial splenectomy. The postoperative course was uneventful in two patients; one patient developed a complication necessitating resection of the rest of the spleen. Haematological parameters improved and oesophageal varices regressed in all patients. On follow up, one patient showed a normal spleen, the other a normally functioning accessory spleen; the third patient again developed splenomegaly with hypersplenism. Partial splenectomy is a promising therapeutic option for CF patients with hypersplenism. PMID:12538318

Thalhammer, G H; Eber, E; Uranüs, S; Pfeifer, J; Zach, M S

2003-02-01

456

Penicillin prophylaxis in children with sickle cell disease in Brent  

Microsoft Academic Search

OBJECTIVE--To assess compliance with oral penicillin prophylaxis in children with sickle cell disease and identify possible reasons for poor compliance. DESIGN--Closed questionnaires given to parents of children with sickle cell disease and general practitioners in Brent. Urine samples from 23 children were tested for penicillin. SETTING--Paediatric haematology clinic, Central Middlesex Hospital, and general practices in Brent. SUBJECTS--50 children (aged less

D Cummins; R Heuschkel; S C Davies

1991-01-01

457

ChlVPP/ABVVP, a first line 'hybrid' combination chemotherapy for advanced Hodgkin's lymphoma: a retrospective analysis.  

PubMed

We retrospectively analysed toxicities and clinical results of 61 Hodgkin's lymphoma patients treated with chlorambucil, vinblastine, procarbazine, doxorubicin, bleomycin, vincristine and etoposide (ChlVPP/ABVVP), delivered in a weekly alternate schedule. Of 61 patients, 33 were in stages III-IV, 21 in stage IIB and seven in stage IIA with bulky disease or extranodal presentation. ChlVPP/ABVVP was administered for 6-8 cycles. Involved field radiotherapy (IFRT) (30-35 Gy) was delivered to 31 patients with residual disease after chemotherapy or bulky disease at diagnosis. Of 61 patients, 58 (95%) achieved complete clinical or radiological remission after chemotherapy and IFRT. With a median follow-up of 60 months, 5-year overall survival, relapse- and event-free survival were 78.8% (95% CI 68.2-91.1%), 81% (95% CI 70.6-92.2%) and 71.9% (95% CI 68.2-82.2%) respectively. Grades 3-4 neutropenia was the most relevant haematological toxicity and occurred in 82% of patients. Non-haematological toxicities were mild and reversible. No toxic deaths were recorded. One patient developed secondary acute myeloid leukaemia 1 year after ChlVPP/ABVVP. Due to the retrospective nature of this study, no definitive conclusions could be drawn about the clinical activity of ChlVPP/ABVVP. Nonetheless, clinical results seem better than those reported with standard regimens [ABVD (doxorubicin, bleomycin, vincristine, dacarbazine), MOPP (methotrexate, vincristine, procarbazine, prednisone), MOPP/ABVD] and as good as those reported using standard or escalated BEACOPP (bleomycin, etoposide, doxorubicin, cyclophosphamide, vincristine, procarbazine and prednisone), with a lower degree of haematological and non-haematological toxicity. Long-term results of the ongoing randomized trial, comparing ABVD versus high-dose intensity weekly regimens will be useful to confirm our results. PMID:15147373

Martinelli, G; Cocorocchio, E; Peccatori, F; Zucca, E; Saletti, P C; Calabrese, L; Pastano, R; Pruneri, G; Mazzetta, C; Ghielmini, M; Cavalli, F

2004-06-01

458

The Austrian Bone Marrow Donor Registry: Providing Patients in Austria with Unrelated Donors for Transplant – a Worldwide Cooperation  

Microsoft Academic Search

SummaryBackground: The Austrian Bone Marrow Donor Registry is the central search coordinating unit in charge of national and international donor searches in Austria. Patients and Methods: Between 1988 and 2010, a worldwide search for an unrelated donor of blood stem cells (URD) was initiated for 2,166 Austrian patients with haematological disorders, 1,671 adults and 495 children, by the Austrian Bone

Agathe Rosenmayr; Margit Pointner-Prager; Martina Winkler; Andrea Mitterschiffthaler; Barbara Pelzmann; Ljiljana Bozic; Sonja Pichler-Kurzweil; Heinz Tüchler; Ingrid Fae; Gottfried Fischer

2011-01-01

459

HLA matching affects clinical outcome of adult patients undergoing haematopoietic SCT from unrelated donors: a study from the Gruppo Italiano Trapianto di Midollo Osseo and Italian Bone Marrow Donor Registry  

Microsoft Academic Search

The importance of HLA donor–recipient matching in unrelated haematopoietic SCT (HSCT) is the subject of debate. In this retrospective study, we analyzed 805 adult patients from the Italian Registry receiving HSCT for a haematological malignancy from January 1999 to June 2006 and correlated the degree of HLA matching with transplant outcome. All patient–donor pairs had high-resolution typing at HLA-A, -B,

R Crocchiolo; F Ciceri; K Fleischhauer; R Oneto; B Bruno; S Pollichieni; N Sacchi; M P Sormani; R Fanin; G Bandini; F Bonifazi; A Bosi; A Rambaldi; P E Alessandrino; M Falda; A Bacigalupo

2009-01-01

460

Current markers of the Athlete Blood Passport do not flag microdose EPO doping  

Microsoft Academic Search

The Athlete Blood Passport is the most recent tool adopted by anti-doping authorities to detect athletes using performance-enhancing\\u000a drugs such as recombinant human erythropoietin (rhEPO). This strategy relies on detecting abnormal variations in haematological\\u000a variables caused by doping, against a background of biological and analytical variability. Ten subjects were given twice weekly\\u000a intravenous injections of rhEPO for up to 12 weeks.

Michael Ashenden; Clare E. Gough; Andrew Garnham; Christopher J. Gore; Ken Sharpe