Sample records for haematology

  1. Haematology and neurology

    Microsoft Academic Search

    Steven Austin; Hannah Cohen; Nick Losseff

    2007-01-01

    This review aims to update the reader on advances in the understanding of haematological conditions that may arise in neurological practice. Thrombophilia, antiphospholipid antibody syndrome, thrombotic thrombocytopenic purpura, sickle cell and clonal disorders associated with neuropathy are discussed.

  2. Automated haematology analysis to diagnose malaria.

    PubMed

    Campuzano-Zuluaga, Germán; Hänscheid, Thomas; Grobusch, Martin P

    2010-01-01

    For more than a decade, flow cytometry-based automated haematology analysers have been studied for malaria diagnosis. Although current haematology analysers are not specifically designed to detect malaria-related abnormalities, most studies have found sensitivities that comply with WHO malaria-diagnostic guidelines, i.e. ? 95% in samples with > 100 parasites/?l. Establishing a correct and early malaria diagnosis is a prerequisite for an adequate treatment and to minimizing adverse outcomes. Expert light microscopy remains the 'gold standard' for malaria diagnosis in most clinical settings. However, it requires an explicit request from clinicians and has variable accuracy. Malaria diagnosis with flow cytometry-based haematology analysers could become an important adjuvant diagnostic tool in the routine laboratory work-up of febrile patients in or returning from malaria-endemic regions. Haematology analysers so far studied for malaria diagnosis are the Cell-Dyn®, Coulter® GEN·S and LH 750, and the Sysmex XE-2100® analysers. For Cell-Dyn analysers, abnormal depolarization events mainly in the lobularity/granularity and other scatter-plots, and various reticulocyte abnormalities have shown overall sensitivities and specificities of 49% to 97% and 61% to 100%, respectively. For the Coulter analysers, a 'malaria factor' using the monocyte and lymphocyte size standard deviations obtained by impedance detection has shown overall sensitivities and specificities of 82% to 98% and 72% to 94%, respectively. For the XE-2100, abnormal patterns in the DIFF, WBC/BASO, and RET-EXT scatter-plots, and pseudoeosinophilia and other abnormal haematological variables have been described, and multivariate diagnostic models have been designed with overall sensitivities and specificities of 86% to 97% and 81% to 98%, respectively. The accuracy for malaria diagnosis may vary according to species, parasite load, immunity and clinical context where the method is applied. Future developments in new haematology analysers such as considerably simplified, robust and inexpensive devices for malaria detection fitted with an automatically generated alert could improve the detection capacity of these instruments and potentially expand their clinical utility in malaria diagnosis. PMID:21118557

  3. Cancer stem cells in haematological malignancies

    PubMed Central

    Golab, Jakub

    2015-01-01

    At least several types of human haematological malignancies can now be seen as ‘stem-cell diseases’. The best-studied in this context is acute myeloid leukaemia (AML). It has been shown that these diseases are driven by a pool of ‘leukaemia stem cells (LSC)’, which remain in the quiescent state, have the capacity to survive and self-renew, and are responsible for the recurrence of cancer after classical chemotherapy. It has been understood that LSC must be eliminated in order to cure patients suffering from haematological cancers. Recent advances in LSC research have allowed for description of LSC phenotype and identification of potential targets for anti-LSC therapies. This concise review summarises the current view on LSC biology and targeted approaches against LSC. PMID:25691816

  4. Cancer stem cells in haematological malignancies.

    PubMed

    Zagozdzon, Radoslaw; Golab, Jakub

    2015-01-01

    At least several types of human haematological malignancies can now be seen as 'stem-cell diseases'. The best-studied in this context is acute myeloid leukaemia (AML). It has been shown that these diseases are driven by a pool of 'leukaemia stem cells (LSC)', which remain in the quiescent state, have the capacity to survive and self-renew, and are responsible for the recurrence of cancer after classical chemotherapy. It has been understood that LSC must be eliminated in order to cure patients suffering from haematological cancers. Recent advances in LSC research have allowed for description of LSC phenotype and identification of potential targets for anti-LSC therapies. This concise review summarises the current view on LSC biology and targeted approaches against LSC. PMID:25691816

  5. [Toward pertinent analytical objectives for haematological parameters].

    PubMed

    Iobagiu, Cristina; Nehar, Diane; Denis, Isabelle; de Saint-Trivier, Aurélie; Boyer, Michelle

    2014-01-01

    Usually, the blood cell counting activity in haematology laboratory uses the comparison of IQC values to the target values proposed by the manufacturer. We intended to improve the monitoring of the proper functioning of our analytical measure system for 17 main haematologic parameters. To set the allowable critical limits of IQC, we propose our reflection based on several elements: benchmark and expert recommendation, clinical requirements, statistical indicators of the laboratory calculated using IQC values (3 levels, 2 different lots, 2 haematology analysers and 2 passage modes) and the EEQ values, during four months. We exploited the reports obtained from the middleware (our own IQC values), and the interlaboratory comparison reports (obtained from SNCS and EuroCell websites) and we compared our performances to the Ricos objectives, to set clearly argued allowable limits for IQC values. Finally, the allowable limits correspond to the imprecision limits stated by Ricos for 14 parameters (desirable for 11 parametres and minimal for 3 parameters) and personalized limits (more exigent than desirable Ricos limits) for 3 parameters of blood cell counting. PMID:25486666

  6. Haematological and Immunological Data Data set n Input

    E-print Network

    Roberts, Stephen

    Haematological and Immunological Data Data set n Input 1 WBC 2 Hgb Haematology 3 MCV 4 Plat 5 Lymph 6 Neut 1 PanT% 2 CD4% Immunology 3 CD8% 4 PanT 5 CD4 6 CD8 slide­1 #12; Data Type Data set KS+ (class 1) KS-- (class 3) Haematology Training 24 82 Test 4 10 Immunology Training 24 81 Test 4 10 KS

  7. Recommendations for the management of the haematological and onco-haematological aspects of Gaucher disease1

    PubMed Central

    Hughes, Derralynn; Cappellini, Maria Domenica; Berger, Marc; Droogenbroeck, Jan Van; de Fost, Maaike; Janic, Dragana; Marinakis, Theodore; Rosenbaum, Hanna; Villarubia, Jesús; Zhukovskaya, Elena; Hollak, Carla

    2007-01-01

    Current knowledge of the haematological and onco-haematological complications of type 1 Gaucher disease has been reviewed with the aim of identifying best clinical practice for treatment and disease management. It was concluded that: (i) Awareness of typical patterns of cytopenia can help clinicians distinguish haematological co-morbidities. (ii) Red blood cell studies and complete iron metabolism evaluation at baseline are recommended. (iii) Haemoglobin levels defining anaemia should be raised and used in Gaucher disease treatment and monitoring. (iv) Surgeons should be aware of potential bleeding complications during surgery in Gaucher patients. The higher incidence of multiple myeloma in Gaucher disease suggests that Gaucher patients should have their immunoglobulin profile determined at diagnosis and monitored every 2 years (patients <50 years) or every year (patients >50 years). If monoclonal gammopathy of undetermined significance (MGUS) is found, general MGUS guidelines should be followed. Future studies should focus on the utility of early treatment to prevent immunoglobulin abnormalities and multiple myeloma. PMID:17655728

  8. Haematological and morphological responses of broiler chicks to hypoxia

    Microsoft Academic Search

    M. H. Maxwell; S. Spence; G. W. Robertson; M. A. Mitchell

    1990-01-01

    Broiler chicks were subjected to experimentally?induced hypoxia and the haematology, together with the histopathology and ultrastructure of heart, liver, lung and kidney and the ultracytochemistry of heart tissues were examined. The haemoglobin concentration, packed cell volume and red blood cell counts were significantly increased compared with controls. The results resembled the haematology of similar aged broilers with an ascitic syndrome

  9. Gaucher disease: haematological presentations and complications.

    PubMed

    Thomas, Alison S; Mehta, Atul; Hughes, Derralynn A

    2014-05-01

    Gaucher disease (GD) is an autosomal recessive lysosomal storage disease, caused by deficiency of the enzyme glucocerebrosidase, required for the degradation of glycosphingolipids. Clinical manifestations include hepatosplenomegaly, thrombocytopenia, bone disease and a bleeding diathesis, frequently resulting in presentation to haematologists. Historically managed by splenectomy, transfusions and orthopaedic surgery, the development of specific therapy in the form of intravenous enzyme replacement therapy in the 1990s has resulted in dramatic improvements in haematological and visceral disease. Recognition of complications, including multiple myeloma and Parkinson disease, has challenged the traditional macrophage-centric view of the pathophysiology of this disorder. The pathways by which enzyme deficiency results in the clinical manifestations of this disorder are poorly understood; altered inflammatory cytokine profiles, bioactive sphingolipid derivatives and alterations in the bone marrow microenvironment have been implicated. Further elucidating these pathways will serve to advance our understanding not only of GD, but of associated disorders. PMID:24588457

  10. Haematological cancer and quality of life: a systematic literature review

    PubMed Central

    Allart-Vorelli, P; Porro, B; Baguet, F; Michel, A; Cousson-Gélie, F

    2015-01-01

    The aim of this study is to examine the impact of haematological cancers on quality of life (QoL). A review of the international literature was conducted from the databases ‘PsycInfo' and 'Medline' using the keywords: 'haematological cancer', 'quality of life', 'physical', 'psychological', 'social', 'vocational', 'professional', 'economic', 'cognitive', and 'sexual'. Twenty-one reliable studies were analysed. Among these studies, 12 showed that haematological cancer altered overall QoL, 8 papers found a deterioration of physical dimension, 8 papers reported on functional and role dimensions, 11 papers reported on the psychological component and 9 on the social component. Moreover, one study and two manuscripts, respectively, reported deteriorated sexual and cognitive dimensions. Our review demonstrates that the different dimensions of QoL are deteriorated by haematological malignancies and, probably, by the side effects of treatment. PMID:25909835

  11. Haematological Reference Intervals in a Multiethnic Population

    PubMed Central

    Ambayya, Angeli; Su, Anselm Ting; Osman, Nadila Haryani; Nik-Samsudin, Nik Rosnita; Khalid, Khadijah; Chang, Kian Meng; Sathar, Jameela; Rajasuriar, Jay Suriar; Yegappan, Subramanian

    2014-01-01

    Introduction Similar to other populations, full blood count reference (FBC) intervals in Malaysia are generally derived from non-Malaysian subjects. However, numerous studies have shown significant differences between and within populations supporting the need for population specific intervals. Methods Two thousand seven hundred twenty five apparently healthy adults comprising all ages, both genders and three principal races were recruited through voluntary participation. FBC was performed on two analysers, Sysmex XE-5000 and Unicel DxH 800, in addition to blood smears and haemoglobin analysis. Serum ferritin, soluble transferrin receptor and C-reactive protein assays were performed in selected subjects. All parameters of qualified subjects were tested for normality followed by determination of reference intervals, measures of central tendency and dispersion along with point estimates for each subgroup. Results Complete data was available in 2440 subjects of whom 56% (907 women and 469 men) were included in reference interval calculation. Compared to other populations there were significant differences for haemoglobin, red blood cell count, platelet count and haematocrit in Malaysians. There were differences between men and women, and between younger and older men; unlike in other populations, haemoglobin was similar in younger and older women. However ethnicity and smoking had little impact. 70% of anemia in premenopausal women, 24% in postmenopausal women and 20% of males is attributable to iron deficiency. There was excellent correlation between Sysmex XE-5000 and Unicel DxH 800. Conclusion Our data confirms the importance of population specific haematological parameters and supports the need for local guidelines rather than adoption of generalised reference intervals and cut-offs. PMID:24642526

  12. Haematological evaluation of naphthalene intoxication in the tropical arcid blood clam Anadara granosa

    Microsoft Academic Search

    J. T. Eapen; B. Patel

    1989-01-01

    In vertebrates haematological parameters are of considerable importance in the diagnosis of pathological disorders due to pollutants. Similar haematological studies in invertebrates are lacking. This is partly because a very few species have erythrocytes; arcid clams, however, are unique in having erythrocytes. Our experiments were designed therefore to evaluate the effect of naphthalene (N) on haematological parameters in the tropical

  13. EFFECT OF CYPERMETHRIN ON CLINICO-HAEMATOLOGICAL PARAMETERS IN RABBITS

    Microsoft Academic Search

    M. KAMAL SHAH; A. KHAN; F. RIZVI; M. SIDDIQUE

    The aim of this study was to know the clinical and haematological changes in female rabbits following the treatment with cypermethrin (CY). In this study, 24 female rabbits were divided randomly into four equal groups A, B, C and D. Groups A, B and C were injected intraperitoneally CY @ 25, 50 and 75 mg\\/Kg b. wt., respectively, while the

  14. Original article Cardio-respiratory, haematological and biochemical

    E-print Network

    Paris-Sud XI, Université de

    period for their cardio-respiratory and metabolic adjustment to strenuous exercise, training and detrainOriginal article Cardio-respiratory, haematological and biochemical parameter adjustments to exercise: effect of a probiotic in horses during training T Art D Votion, K McEntee H Amory, A Linden, R

  15. The Potential Role of Aurora Kinase Inhibitors in Haematological Malignancies

    PubMed Central

    Farag, Sherif S.

    2011-01-01

    Summary Aurora kinases play an important role in the control of the cell cycle and have been implicated in tumourigenesis in a number of cancers. Among the haematological malignancies, overexpression of Aurora kinases has been reported in acute myeloid leukaemia, chronic myeloid leukaemia, acute lymphoblastic leukaemia, multiple myeloma, aggressive non-Hodgkin lymphoma and Hodgkin lymphoma. A large number of Aurora kinase inhibitors are currently in different stages of clinical development. In addition to varying in their selectivity for the different Aurora kinases, some also have activity directed at other cellular kinases involved in important molecular pathways in cancer cells. This review summarizes the biology of Aurora kinases and discusses why they may be good therapeutic targets in different haematological cancers. We describe preclinical data that has served as the rationale for investigating Aurora kinase inhibitors in different haematological malignancies, and summarize published results from early phase clinical trials. While the anti-tumour effects of Aurora kinase inhibitors appear promising, we highlight important issues for future clinical research and suggest that the optimal use of these inhibitors is likely to be in combination with cytotoxic agents already in use for the treatment of various haematological cancers. PMID:21980926

  16. Normal haematology of the houbara bustard ( Chlamydotis undulata macqueenii )

    Microsoft Academic Search

    J. H. Samour; J. C. Howlett; M. G. Hart; T. A. Bailey; J. Naldo; M. A. D'Aloia

    1994-01-01

    Blood samples were collected from 14 male and 20 female clinically normal captive houbara bustards (Chlamydotis undulata macqueenii). Standard haematology assays were carried out to establish normal reference values for the species. No significant statistical differences were observed between males and females. The results obtained are compared with similar studies conducted in the great bustard (Otis tarda).

  17. [Management of treatment-induced pain in paediatric haematology].

    PubMed

    Ben Hamadi, Donia; Calvet, Clémence

    2015-01-01

    Invasive procedures are frequent and painful in children treated in paediatric haematology. It is therefore essential to take into consideration and anticipate the pain induced by these procedures. The caregiver has various effective methods of providing a high quality care management. PMID:26183097

  18. Haematological parameters as bioindicators of insecticide exposure in teleosts

    Microsoft Academic Search

    Narendra Nath Singh; Anil Kumar Srivastava

    2010-01-01

    Haematological parameters, such as erythrocyte and leucocyte count, erythrocyte indices and thrombocyte number vis-a-vis coagulation\\u000a of blood has been considered bioindicators of toxicosis in fish following exposure to organochlorine, organophosphate, carbamate\\u000a and pyrethroid insecticides. This review deals with the effects of insecticides on the morphology of red blood cells, total\\u000a erythrocyte count, haemoglobin content, haematocrit, mean corpuscular volume, mean corpuscular

  19. [Haematological diseases with primary otorhinolaryngological manifestation (author's transl)].

    PubMed

    Tomity, I T; Sütö, M; Nagymajtényi, E; Martinovits, J

    1977-11-01

    There are reports of 10 haematological diseases with primary oto-rhino-laryngological manifestation. The clinical diagnoses were confirmed by laboratory results. The actual diseases were primarily hidden by a tonsillitis, bleeding of the nose after an eye-operation, by an extreme dyspnoe, or by a tumor-like change in the larynx. The authors stress the general necessity of immediate laboratory examinations in dept. of oto-rhinolaryngology. PMID:145533

  20. [Immuno-haematology and blood bank inventory and issue management].

    PubMed

    Madre, F; Benoist, F; Chandesris, C; Nicola, N

    2010-12-01

    Blood bank management must ensure the correct blood product issuance in the right time. For this purpose, patient clinical and immuno-haematological data have to be taken into consideration. Inventory composition, by blood group and phenotype, blood product providing possibilities and transport delays are determining factors. Finally, a good management relies also on the use of consensually written procedures and the monitoring of pertinent indicators. PMID:21067950

  1. Clinical haematology of the great bustard (Otis tarda)

    Microsoft Academic Search

    A. Jimenez; R. Barrera; J. Sanchez; Rafaela Cuenca; J. Rodriguez; S. Andres; María Cinta Mańe

    1991-01-01

    The haematological parameters of healthy great bustards (Otis tarda L.) have been determined. The values obtained were red cell count (3.0 × 10 ± 0.2 × 101), white cell count (33.0 × 10 ± 2.6 × 10\\/1), haematocrit value (0.51 ± 0.01 1\\/1), haemoglobin (13.0 ± 0.3 g\\/dl), mean corpuscular volume (178.7 ± 12.5 fl), mean cell haemoglobin concentration (25.0

  2. Quality Management for Clinical Trials within the German Competence Network Paediatric Oncology and Haematology

    Microsoft Academic Search

    R. Herold; G. Henze; U. Creutzig; M. Zimmermann; J. Hannemann; B. Pfistner

    2005-01-01

    The German ?Competence Network Paediatric Oncology and Haematology? aims at improving the structure of paediatric oncology and haematology as a whole, focussing in particular on the quality of clinical trials and study co-ordinating centres. This comprises the following measures: (1) Employment of research and trial assistants in order to improve the quality of documentation and study management in the participating

  3. Investigation of biochemical and haematological side-effects of cefquinome in healthy dogs.

    PubMed

    Maden, M; Tra?, B; Ba?, A L; Elmas, M; Yazar, E; Birdane, F M

    2001-01-01

    In the present study, the effects of cefquinome, a 4th generation cephalosporin, on clinical, biochemical, haematological, and blood gas variables were investigated. Five healthy dogs were injected with cefquinome (1 mg/kg body weight, IM, daily) for 14 days. Negative effects of cefquinome on clinical, biochemical, and haematological variables were not observed, but it did change some blood gas variables. PMID:11205999

  4. Pharmacology: Investigation of biochemical and haematological side?effects of cefquinome in healthy dogs

    Microsoft Academic Search

    M. Maden; B. Tra?; A. L. Ba?; M. Elmas; E. Yazar; F. M. Birdane

    2001-01-01

    In the present study, the effects of cefquinome, a 4 generation cephalosporin, on clinical, biochemical, haemato?logical, and blood gas variables were investigated. Five healthy dogs were injected with cefquinome (1 mg\\/kg body weight, IM, daily) for 14 days. Negative effects of cefquinome on clinical, biochemical, and haematological variables were not observed, but it did change some blood gas variables.

  5. Transition of care from paediatric to adult services in haematology

    PubMed Central

    Bolton?Maggs, Paula H B

    2007-01-01

    The need for adequate preparation for transition for young people with health care needs who require long term follow?up in the adult sector has long been recognised and is a required part of the national service framework for children. The Royal College of Paediatrics and Child Health and the Royal College of Nursing have endorsed this need for improvement in services for adolescents. In 2006 the Department of Health launched guidelines with a wealth of recommendations. Despite these initiatives only slow progress has been made (usually by enthusiasts) and much work is needed to develop good programmes in many specialties, including non?malignant haematology. PMID:17715443

  6. Atlas of Genetics and Cytogenetics in Oncology and Haematology

    NSDL National Science Digital Library

    The Atlas of Genetics and Cytogenetics in Oncology and Haematology, a peer-reviewed online journal and database provided by Infobiogen, is "devoted to genes, cytogenetics, and clinical entities in cancer, and cancer-prone diseases." Users can search the materials by genes, leukaemias, solid tumors, cancer prone diseases, and chromosomes. Researchers can find links to scientific societies and meetings. Students can explore educational materials on Mendelian and non-Mendelian Inheritance, chromosomes, population genetics, and additional human genetics topics. The website also offers reviews and case reports.

  7. Intestinal helminths induce haematological changes in dogs from Jabalpur, India.

    PubMed

    Qadir, S; Dixit, A K; Dixit, P; Sharma, R L

    2011-12-01

    The effect of canine intestinal helminths on the haematological profile of 200 dogs, of both sexes and variable age, visiting university veterinary clinics for routine examination was investigated. The dogs were assigned to parasitized (n = 39) and non-parasitized (n = 161) groups of animals. Coprological examination revealed a 19.5% prevalence of different species of the helminths. Of these animals, 10.25% had mixed infections with Ancylostoma caninum, Toxascaris spp. and Dipylidium caninum. The intensity of A. caninum infection was the highest, with mean egg counts of 951.43 (standard error 88.66), followed by Toxascaris 283.33 (standard error 116.81) and D. caninum. The parasitized animals had significantly lower levels of haemoglobin, packed cell volume and total erythrocyte counts than non-parasitized animals (P < 0.01). Values of other parameters, except for lymphocytes and eosinophils, were not different between the two groups. Analyses of the haematological profile revealed normocytic hypochromic anaemia in the parasitized group of animals. PMID:21110909

  8. Haematological changes associated with miliary tuberculosis of the bone marrow.

    PubMed

    Lombard, E H; Mansvelt, E P

    1993-04-01

    The peripheral blood and bone marrow findings in 25 patients found to have tuberculous granulomata on bone marrow examination were examined to determine whether specific haematological findings are associated with tuberculous infiltration of the bone marrow. All the patients had one abnormality or more on their full blood count. The presence of a peripheral lymphopaenia was the single factor common to all 25 patients studied. The peripheral lymphopaenia was only associated with bone marrow lymphopaenia in 14% of patients. Other findings of note were an association of peripheral monocytopaenia and absence of giant cells in the granulomata, and decreased iron stores in almost a third of the total number of patients. The relevance of the lymphopaenia, monocytopaenia and decreased iron stores are discussed and we propose that the absence of a peripheral lymphopaenia makes it very unlikely that there will be tuberculous localization in the bone marrow. PMID:8324206

  9. Haematology and red cell enzymes of the Australian orange-bellied parrot, Neophema chrysogaster

    Microsoft Academic Search

    W. D. Melrose; P. B. Brown; M. C. Holdsworth; S. L. Bryant

    1995-01-01

    The haematology, red cell enzymes, and haemoglobin electrophoresis of twelve captive bred Australian orange-bellied parrots (Neophema chrysogaster) were studied. The means of the haematological parameters were: haemoglobin 15.3 g\\/dl, PCV 54%, red cell count 3.4 × 1012\\/l, MCV 138.0 fl, MCH 44.7 pg, leucocyte count 7.2 × 109\\/l, heterophils 61%, lymphocytes 24%, monocytes 6.3%, eosinophils 0.7% and basophils 6.8%. The

  10. Comparison of some haematological parameters between three bird Comparison of some haematological parameters between three bird species from the species from the Columbidae Columbidae family - family - short communication

    Microsoft Academic Search

    Lubomir Lashev; Huben Hubenov; Yordan Nikolov; Valentina Lasheva; Radoslav Mihailov

    Investigations into some haematological values in birds from three Columbidae species e.g. pigeons, (Columba livia domestica), collared doves (Streptopelia decaocto) and African collared doves, (Streptopelia roseogrisea) have been performed. In all three species, high haemoglobin concentrations, and respectively high red blood cell counts were observed. In S. decaocto the red blood cell count was extremely high, corresponding to the high

  11. Peromyscus leucopus mice: a potential animal model for haematological studies.

    PubMed

    Sun, Yu; Desierto, Marie J; Ueda, Yasutaka; Kajigaya, Sachiko; Chen, Jichun; Young, Neal S

    2014-10-01

    Peromyscus leucopus mice share physical similarities with laboratory mice Mus musculus (MM) but have higher agility and longer lifespan. We compared domesticated P. leucopus linville (PLL) and M. musculus C57BL/6 (MMB6) mice for cellular composition of peripheral blood (PB), bone marrow (BM) and spleen. PLL mice had significantly fewer platelets and significantly more monocytes in the blood, and notably fewer megakaryocytes in the BM. Spleens of PLL mice were significantly smaller, with 50% fewer cells and reduced 'red pulp'. There was no obvious haematological change in PLL mice between 2-8 and 16-26 months of age, except for a significant increase in blood monocytes. Cellular reactive oxygen species (ROS) content showed no change with age but differed significantly between different cell types. Treating two to eight month-old PLL mice with antioxidant N-acetylcysteine in drinking water for three months did not affect cellular ROS content, but increased blood leucocytes especially the concentration of monocytes. The low platelets, low megakaryocytes, high monocytes and low splenic erythropoiesis in PLL mice resemble human measurements better than the values seen in MMB6. PMID:25116892

  12. Basic haematological values in carnivores--II. The Felidae.

    PubMed

    Pospísil, J; Kase, F; Váhala, J

    1987-01-01

    1. Basic haematological values in 34 animals of eight carnivorous species are reported. 2. In four Northern lynxs (Lynx lynx lynx), two male and two female animals, the mean values are given: erythrocyte counts 8.51 X 10(12)/l, haematocrit 0.392/l, haemoglobin content 148.0 g/l and leukocyte count 7.92 X 10(9)/l. 3. In six male pumas (Puma concolor missolensis) the mean values estimated are: erythrocyte count 9.35 X 10(12)/l, haematocrit 0.43/l, haemoglobin content 163.9 g/l and leukocyte count 7.73 X 10(9)/l. Individual values in one female puma are also given. 4. In six jaguars (Panthera onca), three male and three female animals, the mean values are given: erythrocyte count 8.27 X 10(12)/l, haematocrit 0.37/l, haemoglobin content 137.1 g/l and leukocyte count 15.15 X 10(9)/l. 5. Only individual values are reported in one clouded leopard (Neofelis nebulosa), in one leopard (Panthera pardus saxicolor), in one Corbett's tiger (Panthera tigris Corbetti) and in one Altaic tiger (Panthera tigris Altaica). 6. In four lions (Panthera leo leo), two male and two female animals, the mean estimated values are: erythrocyte count 10.14 X 10(12)/l, haematocrit 0.462/l, haemoglobin content 159.0 g/l and leukocyte count 11.05 X 10(9)/l. In six female cheetahs (Acinonox jubatus jubatus) the mean values estimated are: erythrocyte count 7.86 X 10(12)/l, haematocrit 0.373/l, haemoglobin content 142.8 g/l and leukocyte count 8.65 X 10(9)/l. For three male cheetahs only individual values are reported. 8. All results achieved are compared with those abstracted from the literature and discussed. PMID:2886279

  13. Haematological and Biochemical Values or the Blood and Blood Serum of Captive Northern White Rhinoceroses (Ceratotherium simum cottoni)

    Microsoft Academic Search

    J. Váhala; V. Kaše; O. A. Ryder

    1994-01-01

    Va h a I a 1., F. K d e and O. A. R Y d e r: Haematological and Biochemical Values of the Blood and Blood Serum of Captive Northern White Rhinoceroses (Ceratotherium simum cottoni). Acta vet. Brno, 63,1994: 99-102. Haematological and biochemical blood and blood serum values obtained from 7 northern white rhinoceroses (Ceratotherium simum cottoni) kept in

  14. Support vector machine versus logistic regression modeling for prediction of hospital mortality in critically ill patients with haematological malignancies

    Microsoft Academic Search

    T Verplancke; S Van Looy; D Benoit; S Vansteelandt; P Depuydt; F De Turck; J Decruyenaere

    2008-01-01

    BACKGROUND: Several models for mortality prediction have been constructed for critically ill patients with haematological malignancies in recent years. These models have proven to be equally or more accurate in predicting hospital mortality in patients with haematological malignancies than ICU severity of illness scores such as the APACHE II or SAPS II 1. The objective of this study is to

  15. The prevention and management of infections due to multidrug resistant organisms in haematology patients.

    PubMed

    Trubiano, Jason A; Worth, Leon J; Thursky, Karin A; Slavin, Monica A

    2015-02-01

    Infections due to resistant and multidrug resistant (MDR) organisms in haematology patients and haematopoietic stem cell transplant recipients are an increasingly complex problem of global concern. We outline the burden of illness and epidemiology of resistant organisms such as gram-negative pathogens, vancomycin-resistant Enterococcus faecium (VRE), and Clostridium difficile in haematology cohorts. Intervention strategies aimed at reducing the impact of these organisms are reviewed: infection prevention programmes, screening and fluoroquinolone prophylaxis. The role of newer therapies (e.g. linezolid, daptomycin and tigecycline) for treatment of resistant and MDR organisms in haematology populations is evaluated, in addition to the mobilization of older agents (e.g. colistin, pristinamycin and fosfomycin) and the potential benefit of combination regimens. PMID:24341410

  16. Haematological and serum biochemical responses of chickens to hydric stress.

    PubMed

    Chikumba, N; Swatson, H; Chimonyo, M

    2013-09-01

    Dehydration can be extremely damaging to the performance and welfare of indigenous chickens. The effect of water restriction on haematological and biochemical parameters was compared in Naked Neck (NNK) and Ovambo (OVB) chickens. A total of 54 8-week-old pullets each of NNK and OVB chickens with an initial average weight of 641 ± 10 g/bird were randomly assigned to three water intake treatments with three replications, each having six birds. The water restriction treatments were ad libitum, 70% and 40% of ad libitum intake. Nine experimental pens with a floor space of 3.3 m2 per strain were used. Feed was provided ad libitum. Packed cell volume (PCV), erythrocyte count (RBC), mean corpuscular volume (MCV) and total leucocyte count (WBC), and biochemical parameters (uric acid (UA)), creatinine (CREAT), total protein (TP), albumin (ALB), globulin (GLOB), triglyceride (TGA), total cholesterol (TC), high- (HDLC) and low- (LDLC) density lipoprotein cholesterol and activity of alanine transaminase (ALT), alkaline phosphatase (ALP) and aspartate transaminase (AST) were determined from blood collected after 60 days of water restriction. PCV was higher (P < 0.05) in NNK than OVB chickens offered water ad libitum, but similar in birds offered 70% and 40% of ad libitum. There were no differences in RBC and MCV values between strains, but MCV was higher in birds on 40% than 70% of ad libitum water intake, irrespective of strain. Naked neck chickens had higher (P < 0.05) WBC values than OVB at 40% restriction level, but lower WBC than OVB at 70% water restriction level. UA, CREAT, TGA, TC, LDLC, TP and GLOB increased (P < 0.05) with each increment in water restriction, but the increase in CREAT and TC was more pronounced in OVB than NNK chickens. The opposite was observed for UA. ALT activity indicated that liver function was not affected by water restriction. It was concluded that the two strains can withstand up to 40% of ad libitum water restriction, but NNK tolerated water stress better than OVB chickens. PMID:23764254

  17. Haematological evaluation of patients bitten by the jararaca, Bothrops jararaca, in Brazil

    Microsoft Academic Search

    Marcelo L. Santoro; Ida S. Sano-Martins; Hui W. Fan; Joăo L. C. Cardoso; R. David G. Theakston; David A. Warrell

    2008-01-01

    Complete blood counts are used frequently by physicians to assess and manage the development of complications of diseases. We studied 100 patients bitten by Bothrops jararaca snakes, and correlated their haematological values with the severity of envenoming and the development of complications. Patients who developed both local and systemic bleeding showed a greater drop in packed cell volume, red blood

  18. STUDY OF EFFECTS ON HAEMATOLOGICAL PARAMETERS OF RABBITS INTRATRACHEALLY EXPOSED TO COAL FLY ASH

    Microsoft Academic Search

    V. O. Ogugbuaja; P. A. Onyeyili; E. A. Moses

    2001-01-01

    This study was undertaken to evaluate the effects of the inorganic constituents of coal on the haematological parameters of rabbits. Coal obtained from mines in Enugu, Nigeria was ashed at temperature of 900°C and the ash was fractionated to obtain a size range of 4–5 ?m mean particle diameter. Rabbits were exposed to 50, 100 and 200 mg per kilogram

  19. Allogeneic haematopoietic stem cell transplantation (HSCT) is the only curative option for many haematological

    E-print Network

    Cai, Long

    haematological malignancies. However, the development of graft-versus- host disease (GVHD) limits the success, haematopoietic graft source and GVHD pro- phylaxis approaches. Steroids are the first line of treat- ment-HSCT -- is now known to have a characteristic clinical presentation, which resembles autoimmune vascular diseases

  20. Haematological response of curimbas Prochilodus lineatus, naturally infected with Neoechinorhynchus curemai.

    PubMed

    Belo, M A A; Souza, D G F; Faria, V P; Prado, E J R; Moraes, F R; Onaka, E M

    2013-04-01

    This study evaluated the haematological response of curimbas Prochilodus lineatus, naturally infected with Neoechinorhynchus curemai (Acanthocephala: Neoechinorhynchidae). Thirty-seven fish were captured in October 2010 from the Mogi Guaçu River, Porto Ferreira, SP, Brazil. Infected fish presented increased mean corpuscular volume of erythrocytes, and lower thrombocyte and higher monocyte counts than uninfected fish. PMID:23557315

  1. Effect of malarial infection on haematological parameters in population near Thailand-Myanmar border

    PubMed Central

    2014-01-01

    Background Malaria is a major mosquito-borne public health problem in Thailand with varied haematological consequences. The study sought to elucidate the haematological changes in people who suspected malaria infection and their possible predictive values of malaria infection. Methods Haematological parameters of 4,985 patients, including 703 malaria-infected and 4,282 non-malaria infected, who admitted at Phop Phra Hospital, Tak Province, an area of malaria endemic transmission in Thailand during 2009 were evaluated. Results The following parameters were significantly lower in malaria-infected patients; red blood cells (RBCs) count, haemoglobin (Hb), platelets count, white blood cells (WBCs) count, neutrophil, monocyte, lymphocyte and eosinophil counts, while mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), Mean corpuscular haemoglobin concentration (MCHC), neutrophil-lymphocyte ratio (NLR), and monocyte-lymphocyte ratio (MLR) were higher in comparison to non-malaria infected patients. Patients with platelet counts?haematological parameters with low platelet, WBCs, and lymphocyte counts being the most important predictors of malaria infection. When used in combination with other clinical and microscopy methods, these parameters could improve malaria diagnosis and treatment. PMID:24898891

  2. Evaluation of the Sysmex pocH-1001 haematology analyser in an outdoor oncology service.

    PubMed

    Van Hecke, I; Vanden Bempt, I; Malfait, R; Van Den Bossche, J

    2010-01-01

    Since rapid blood count analysis as near patient testing is expanding, we evaluated the use of a Sysmex pocH-100i compact haematology analyser in an outdoor oncology setting according to the recently published International Council for Standardization in Haematology (ICSH) guidelines. In total, 838 blood samples from oncology patients were analysed by pocH-100i and re-analysed by a high-throughput haematology analyser for comparison (Abbott CD-4000 or Sysmex XE-2100) to evaluate in use imprecision, comparability and vote-outs. Imprecision was less than 5%, except for platelet enumeration in the low range (within-run imprecision 7%). Good comparability was found even for platelet enumeration in the low range (r2 = 0.82). Vote-outs were found in 10.6% of examined samples. In conclusion, the Sysmex pocH-100i demonstrates good imprecision conform with former publications, produces reliable results in normal and in lower ranges comparable to the results of high throughput haematology analysers. In a well controlled management plan the Sysmex pocH-100i is suitable for near patient testing in oncology. PMID:20954464

  3. Haematological, coagulation and blood chemistry data in red-bellied tamarins Saguinus labiatus

    Microsoft Academic Search

    Peter F. Wadsworth; William A. Hiddleston; David V. Jones; John S. L. Fowler; Robert A. Ferguson

    1982-01-01

    Summary Haematology, coagulation and clinical chemistry data are reported for a group of male and female red-bellied tamarins (Saguinus labiatus). The tamarins were juvenile and young adults and were bred in captivity. High mean values for activities of alkaline phosphatase, alanine amino-transferase, aspartate aminotransferase and creatine kinase were noted. The findings are compared with data obtained from other members of

  4. Variation in survival of adult patients with haematological malignancies in Europe since 1978

    Microsoft Academic Search

    P. M. Carli; J. W. W. Coebergh; A. Verdecchia

    1998-01-01

    Data on 73070 patients for seven major haematological malignancies diagnosed in Europe between 1985 and 1989 from 39 population-based cancer registries in 17 countries are included in the EUROCARE database. Relative survival was analysed by country and age between 1985 and 1989 and time trends were analysed from 1978–1989 for 13 countries which collaborated in EUROCARE for this entire period.

  5. mTOR Inhibitors and Their Potential Role in Therapy in Leukemia and Other Haematologic Malignancies

    PubMed Central

    Teachey, David T.; Grupp, Stephan A.; Brown, Valerie I.

    2009-01-01

    Summary The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that functions as a key regulator of cell growth, protein synthesis, and cell-cycle progression through interactions with a number of signaling pathways, including PI3K/AKT, ras, TCL1, and BCR/ABL. Many haematologic malignancies have aberrant activation of the mTOR and related signaling pathways. Accordingly, mTOR inhibitors, a class of signal transduction inhibitors that were originally developed as immunosuppressive agents, are being investigated in preclinical models and clinical trials for a number of haematologic malignancies. Sirolimus and second generation mTOR inhibitors such as temsirolimus and everolimus, are safe and relatively well-tolerated, making them potentially attractive as single agents or in combination with conventional cytotoxics and other targeted therapies. Promising early clinical data suggests activity of mTOR inhibitors in a number of haematologic diseases, including acute lymphoblastic leukemia, chronic myelogenous leukemia, mantle cell lymphoma, anaplastic large cell lymphoma, and lymphoproliferative disorders. This review describes the rationale for using mTOR inhibitors in a variety of haematologic diseases with a focus on their use in leukemia. PMID:19344392

  6. Studies on an ascitic syndrome in young broilers 1. haematology and pathology

    Microsoft Academic Search

    M. H. Maxwell; G. W. Robertson; S. Spence

    1986-01-01

    In the present investigation, the haematology and pathology of young broiler chicks with an ascitic syndrome were examined.Gross changes included cardiomegaly, ascites, congested lungs, shrunken livers, enlarged kidneys, dark breast muscle and congested intestines. Many blood parameters were significantly raised including the packed cell volume (PCV), haemoglobin (Hb), red (Rbc) and white blood cell (Wbc) counts. The heterophils and monocytes

  7. Normal haematological values of European hedgehogs (Erinaceus europaeus) from an English rehabilitation centre.

    PubMed

    Lewis, J C M; Norcott, M R; Frost, L M; Cusdin, P

    2002-11-01

    Blood samples were taken from 29 male and 21 female clinically normal European hedgehogs (Erinaceus europaeus) that had been overwintered in an English rehabilitation centre, and the mean (sd) and ranges of their haematological values were determined. The mean cellular volume and lymphocyte counts of the female hedgehogs were slightly but significantly higher than those of the male hedgehogs. PMID:12452356

  8. Haematological factors associated with proliferative retinopathy in homozygous sickle cell disease

    Microsoft Academic Search

    R. J. Hayes; P. I. Condon; G. R. Serjeant

    1981-01-01

    Haematological indices were investigated in 261 patients with homozygous sickle cell disease, 29 of whom had developed proliferative sickle retinopathy (PSR). After allowing for age-related effects, male patients with high Hb (> 9 g\\/dl) and low HbF (< 5%) levels appeared to constitute a high risk group for PSR. This relationship was not evident in females, PSR being observed in

  9. Where are we at with point-of-care testing in haematology?

    PubMed

    Briggs, Carol; Kimber, Simon; Green, Laura

    2012-09-01

    Point-of-care testing (POCT) in haematology has continued to grow in popularity and uptake throughout the world. The increasing demand to reduce the turnaround time of test results, coupled with rapid improvements in technology, have led to the development of several devices that are designed for use in different clinical settings, with the hope of improving patient care. The most used POCT in haematology is measurement of haemoglobin concentration. Other POCT devices (used primarily in developing countries) for malaria screening and CD4+ T-lymphocytes for quantification of human-immunodeficiency-virus are becoming the cornerstone for the diagnosis and management of these disorders. New devices are also available for red cell indices, white blood cell count and platelets. In this review clinical studies that validate the use of such devices will be discussed, as well as the advantages and disadvantages of POCT in haematology. A disadvantage of POCT is a lack of training, poor standardization in obtaining blood samples and insufficient internal/external quality assessment. As there is every reason to expect that POCT use will increase in all pathology disciplines, including haematology, it is imperative that systems are put in place to oversee these issues. PMID:22765160

  10. Itraconazole for prophylaxis of systemic mycoses in neutropenic patients with haematological malignancies

    Microsoft Academic Search

    A. Bonnie; G. Just-Nübling; L. Bergmann; P. M. Shah; W. Stille; D. Hoelzer

    1996-01-01

    The efficacy of oral itraconazole 2 x 200 mg capsules daily for prevention of systemic mycoses was investigated in granulocytopenic patients with haematological malignancies. Of 241 patients, 197 were evaluable for prophylactic efficacy, and 214 for adverse events. Patients with similar characteristics receiving oral amphotencin B as antifungal prophylaxis, observed over 15 months before introduction of itraconazole, served as control

  11. Prevention of doxorubicin-induced myocardial and haematological toxicities in rats by the iron chelator desferrioxamine

    Microsoft Academic Search

    M. M. AI-Harbil; Naji M. Al-Gharably; Othman A. AI-Shabanahl; Abdullah M. Al-Bekairi; Abdel Moneim M. Osman; Hassan N. Tawfik

    1992-01-01

    Biochemical and histopathological evaluations of the protective effects of the iron-chelator desferrioxamine against the cardiac and haematological toxicities of doxorubicin in normal rats were carried out. A single dose of doxorubicin (15 mg\\/kg, i. v.) caused myocardial damage that manifested biochemically as an elevation of serum cardiac enzyme [glutamic oxaloacetic transaminase (GOT), lactic dehydrogenase (LDH) and creatine phosphokinase (CPK)] and

  12. Haematological and biochemical reference intervals for free-ranging brown bears (Ursus arctos) in Sweden

    PubMed Central

    2014-01-01

    Background Establishment of haematological and biochemical reference intervals is important to assess health of animals on individual and population level. Reference intervals for 13 haematological and 34 biochemical variables were established based on 88 apparently healthy free-ranging brown bears (39 males and 49 females) in Sweden. The animals were chemically immobilised by darting from a helicopter with a combination of medetomidine, tiletamine and zolazepam in April and May 2006–2012 in the county of Dalarna, Sweden. Venous blood samples were collected during anaesthesia for radio collaring and marking for ecological studies. For each of the variables, the reference interval was described based on the 95% confidence interval, and differences due to host characteristics sex and age were included if detected. To our knowledge, this is the first report of reference intervals for free-ranging brown bears in Sweden. Results The following variables were not affected by host characteristics: red blood cell, white blood cell, monocyte and platelet count, alanine transaminase, amylase, bilirubin, free fatty acids, glucose, calcium, chloride, potassium, and cortisol. Age differences were seen for the majority of the haematological variables, whereas sex influenced only mean corpuscular haemoglobin concentration, aspartate aminotransferase, lipase, lactate dehydrogenase, ?-globulin, bile acids, triglycerides and sodium. Conclusions The biochemical and haematological reference intervals provided and the differences due to host factors age and gender can be useful for evaluation of health status in free-ranging European brown bears. PMID:25139149

  13. A prospective, cohort, multicentre study of candidaemia in hospitalized adult patients with haematological malignancies.

    PubMed

    Gamaletsou, M N; Walsh, T J; Zaoutis, T; Pagoni, M; Kotsopoulou, M; Voulgarelis, M; Panayiotidis, P; Vassilakopoulos, T; Angelopoulou, M K; Marangos, M; Spyridonidis, A; Kofteridis, D; Pouli, A; Sotiropoulos, D; Matsouka, P; Argyropoulou, A; Perloretzou, S; Leckerman, K; Manaka, A; Oikonomopoulos, P; Daikos, G; Petrikkos, G; Sipsas, N V

    2014-01-01

    Invasive candidiasis is a life-threatening infection in patients with haematological malignancies. The objective of our study was to determine the incidence, microbiological characteristics and clinical outcome of candidaemia among hospitalized adult patients with haematological malignancies. This is a population-based, prospective, multicentre study of patients ? 18 years admitted to haematology and/or haematopoietic stem cell transplantation units of nine tertiary care Greek hospitals from January 2009 through to February 2012. Within this cohort, we conducted a nested case-control study to determine the risk factors for candidaemia. Stepwise logistic regression was used to identify independent predictors of 28-day mortality. Candidaemia was detected in 40 of 27,864 patients with haematological malignancies vs. 967 of 1,158,018 non-haematology patients for an incidence of 1.4 cases/1000 admissions vs. 0.83/1000 respectively (p <0.001). Candidaemia was caused predominantly (35/40, 87.5%) by non-Candida albicans species, particularly Candida parapsilosis (20/40, 50%). In vitro resistance to at least one antifungal agent was observed in 27% of Candida isolates. Twenty-one patients (53%) developed breakthrough candidaemia while receiving antifungal agents. Central venous catheters, hypogammaglobulinaemia and a high APACHE II score were independent risk factors for the development of candidaemia. Crude mortality at day 28 was greater in those with candidaemia than in control cases (18/40 (45%) vs. 9/80 (11%); p <0.0001). In conclusion, despite antifungal prophylaxis, candidaemia is a relatively frequent infection associated with high mortality caused by non-C. albicans spp., especially C. parapsilosis. Central venous catheters and hypogammaglobulinaemia are independent risk factors for candidaemia that provide potential targets for improving the outcome. PMID:23889746

  14. Occupational exposures and haematological abnormalities among ordnance factory workers: a case control study.

    PubMed

    West, R R; Stafford, D A

    1997-07-01

    The lifetime exposures to an extensive list of chemical and other potentially toxic hazards were estimated for all available employees at an ordnance factory by questionnaire-based interview. Exposure histories of 32 (of 33) previously diagnosed as haematologically abnormal (cases) were compared with 322 (of 345) normals (controls). Among 'ordnance factory chemicals', modestly increased odds ratios were observed for men for acetic anhydride (2.8), stearic acid (2.8), and possibly for resorcinol (2.9), TNT (2.4) and hydroxy terminated polybutadene (HTPB) (2.4). Increased odds ratios were also observed in exposures not directly related to ordnance manufacture, including mineral acids, welding fumes, exhaust gases and insecticides. While, small numbers in the case group limit the statistical significance of reported odds ratios, one should be cautious about committing a type II error. These findings may partly explain the previously reported unusual prevalence of haematological abnormalities within the factory. PMID:9301689

  15. Clinical, haematological, and neurocognitive findings in lead-exposed workers of a battery plant in Iran.

    PubMed

    Malekirad, Ali Akbar; Kalantari-Dehaghi, Razieh; Abdollahi, Mohammad

    2013-12-01

    The aim of this comparative cross-sectional study was to assess neurocognitive impairment, haematological findings, and clinical symptoms of lead exposure among 316 male battery plant workers aged between 20 and 61 years. Compared to 123 matched controls (matching in age and years of work), the exposed workers showed significantly higher mean blood lead level (BLL) and lower haematocrit, haemoglobin, and red blood cell count. Mean BLL significantly correlated with clinical symptoms such as nocturia, increased urination frequency, oedema, drop in deep tendon reflex, concentration impairment, agitation, headache, depression, abdominal pain, palpitation, fatigue, and diminished sex drive. Workers with clinical disorders had higher BLL and lower haematological parameters. These findings warn that silent toxicological problems caused by lead might go unnoticed by health professionals. PMID:24384755

  16. Clinical, haematological and therapeutic studies on tropical theileriosis in water buffaloes ( Bubalus bubalis) in Egypt

    Microsoft Academic Search

    Salama A. Osman; Magdy H. Al-Gaabary

    2007-01-01

    Thirty buffaloes naturally infected with Theileria annulata and 10 parasitologically free controls were used to determine the potential clinical, haematological and therapeutic impact of tropical theileriosis in Egypt. The clinical signs in the infected buffaloes were pyrexia (40.5–41.5°C), enlargement of superficial lymph nodes, slight nasal and ocular discharges, salivation, anaemia and respiratory distress. Eye lesions also were recorded. There was

  17. Effects of lichen extracts on haematological parameters of rats with experimental insulin-dependent diabetes mellitus.

    PubMed

    Colak, Suat; Geyiko?lu, Fatime; Aslan, Ali; Deniz, Gül?ah Y?ld?z

    2014-11-01

    The prevalence of diabetes mellitus in the world is steadily increasing. Oxidative stress contributes to the development of diabetic complications, including diabetic haematological changes. Lichens are used as food supplements and are also used as possible natural antioxidant, antimicrobial and anticancer agents. We hypothesized that antioxidant activity of lichens may decrease hyperglycaemia-induced oxidative stress and prevent the development of diabetic complications, including abnormality in haematological condition. Therefore, the effects of Cetraria islandica water extract (CIWE) and Pseudevernia furfuracea water extract (PFWE) on the haematological parameters of rats with type 1 DM were investigated for the first time in the present study. Control Sprague-Dawley or streptozotocin (STZ)-induced diabetic rats were either untreated or treated with water lichen extracts (5-500 mg/kg body weight (bw)/day) for 2 weeks, starting at 72 h after STZ injection. On day 14, animals were anaesthetized and haematological and metabolic parameters were determined between control and experimental groups. In addition, the total oxidative stress (TOS), a specific indicator of oxidative stress, and the total antioxidant capacity (TAC) were measured by biochemical studies. In diabetic rats, CIWE of 250-500 mg/kg bw dose showed more prominent results when compared with doses of PFWE for TAC. The results obtained in the present study suggested that the antioxidant activities of lichens might be the possible reason behind the observed antihaematological status. However, the protective effect of lichen extracts were inadequate on diabetes-induced microcytic hypochromic anaemia. In addition, the extracts have no effect on metabolic complications. Our experimental data showed that high doses of CIWE and PFWE alone have no detrimental effect on blood cells and TOS status of plasma. Hence, they are safe and suitable for different administration routes. PMID:23114377

  18. Clinical performance of leukocyte differential on the new Roche Cobas Vega haematological analyzer.

    PubMed

    Lippi, G; Nicoli, M; Modena, N; Guidi, G

    1997-02-01

    The Roche Cobas Vega is a five-part differential haematological analyzer evolving from the former Cobas Helios, Argos and Micros. As the leukocyte differential of Cobas Vega displays several interesting features, we analyzed its clinical performance and compared it either to three other commercial haematological analyzers (Technicon-Bayer H*2, Coulter STKS and Abbott CD-3500) or to the manual reference method, as described in the National Committee for Clinical Laboratory Standards (NCCLS) H20-A protocol. Within- and between-batch coefficients of variations (CVs) of the white blood cell differential were satisfactory and close, or even better than the ones reported for the other instruments: 0.81% (neutrophils), 1.87% (lymphocytes), 6.76% (monocytes), 7.73% (eosinophils) and 22.55% (basophils). The identification of abnormalities in the white blood cell differential was obtained either on the basis of the instrument-specific ranges or on the generation of one or more flags by comparison with results of the manual reference method. The Vega demonstrated remarkable performance in terms of specificity (90.0%), sensitivity (91.6%) and both positive (97.7%) and negative (69.1%) predictive values; likewise, the global efficiency was the best (90.3%) among the analyzers we evaluated. The flags generated in the presence of morphological abnormalities of the samples displayed excellent sensitivity (from 88.9% to 100%), specificity (from 93.5% to 98.3%) and satisfactory positive (from 51.1% to 66.7%) and negative (from 98.3% to 100%) predictive values. We conclude that the analytical performance of the new Cobas Vega haematological analyzer can significantly improve the identification of various haematological abnormalities as an important contribution to the accuracy is provided by the new staining for granulocytes. PMID:9056752

  19. Age-related haematology changes in captive-reared kori bustards ( Ardeotis kori )

    Microsoft Academic Search

    J. C. Howlett; J. H. Samour; T. A. Bailey; J. L. Naldo

    1998-01-01

    Blood samples were collected from clinically normal, captive-reared, chick and juvenile kori bustards (Ardeotis kori), at regular intervals, over a 15-month period. Full routine haematological analyses were carried out to establish reference\\u000a values for growing individuals of this species and to assess age-related changes. The results of this study showed significant\\u000a age-related differences in haematrocrit, haemoglobin and red blood cell

  20. Haematological Changes During Normal Pregnancy in New Zealand White Rabbits: A Longitudinal Study

    Microsoft Academic Search

    J.-C. KIM; H.-I. YUN; S.-W. CHA; K.-H. KIM; W. S. KOH; M.-K. CHUNG

    2002-01-01

    :   The present study was undertaken to investigate changes in haematology parameters over the course of normal pregnancy in\\u000a New Zealand White rabbits. Blood samples were collected on gestational days (GD) 0, 4, 8, 12, 16, 20, 24, and 28. Red blood\\u000a cell counts and haemoglobin concentrations on GD 20–28 were lower than those of normal non-pregnant rabbits. These values

  1. Virologic response and haematologic toxicity of boceprevir- and telaprevir-containing regimens in actual clinical settings.

    PubMed

    Butt, A A; Yan, P; Shaikh, O S; Freiberg, M S; Lo Re, V; Justice, A C; Sherman, K E

    2015-09-01

    Effectiveness, safety and tolerability of boceprevir (BOC) and telaprevir (TPV) in actual clinical settings remain unknown. We determined rates of sustained virologic response (SVR) and haematologic adverse effects among persons treated with BOC- or TPV-containing regimens, compared with pegylated interferon/ribavirin (PEG/RBV). Using an established cohort of hepatitis C virus (HCV)-infected persons, Electronically Retrieved Cohort of HCV Infected Veterans (ERCHIVES), we identified those treated with a BOC- or TPV-containing regimen and HCV genotype 1-infected controls treated with PEG/RBV. We excluded those with HIV coinfection and missing HCV RNA values to determine SVR. Primary endpoints were SVR (undetectable HCV RNA ?12 weeks after treatment completion) and haematologic toxicity (grade 3/4 anaemia, neutropenia and thrombocytopenia). We evaluated 2288 persons on BOC-, 409 on TPV-containing regimen and 6308 on PEG/RBV. Among these groups, respectively, 31%, 43% and 9% were treatment-experienced; 17%, 37% and 14% had baseline cirrhosis; 63%, 54% and 48% were genotype 1a. SVR rates among noncirrhotics were as follows: treatment naďve: 65% (BOC), 67% (TPV) and 31% (PEG/RBV); treatment experienced: 57% (BOC), 54% (TPV) and 13% (PEG/RBV); (P-value not significant for BOC vs TPV; P < 0.0001 for BOC or TPV vs PEG/RBV). Haematologic toxicities among BOC-, TPV- and PEG/RBV-treated groups were as follows: grade 3/4 anaemia 7%, 11% and 3%; grade 4 thrombocytopenia 2.2%, 5.4% and 1.7%; grade 4 neutropenia 8.2%, 5.6% and 3.4%. SVR rates are higher and closer to those reported in pivotal clinical trials among BOC- and TPV-treated persons compared with PEG/RBV-treated persons. Haematologic adverse events are frequent, but severe toxicity is uncommon. PMID:25524834

  2. Normal haematology and age-related findings in the rufous-crested bustard ( Eupodotis ruficrista )

    Microsoft Academic Search

    M. A. D'Aloia; J. C. Howlett; J. H. Samour; T. A. Bailey; J. Naldo

    1995-01-01

    Blood samples were collected from six juvenile (age 4–6 months) and 14 adult (two years and more) clinically normal captive rufous-crested bustards (Eupodotis ruficrista). Standard haematology assays were carried out to establish normal reference values for the species and to investigate any age-related differences. Compared with adults, juveniles showed low haemoglobin levels, mean cell haemoglobin and mean cell haemoglobin concentrations

  3. Incidence of haematological malignancies by ethnic group in England, 2001-7.

    PubMed

    Shirley, Megan H; Sayeed, Shameq; Barnes, Isobel; Finlayson, Alexander; Ali, Raghib

    2013-11-01

    The aetiology of most haematological malignancies is largely unknown. Studies of migrant populations can provide insights into the relative importance of genetic and environmental risk factors for these diseases. This study compares incidence rates in British Indians, Pakistanis, Bangladeshis, Black Africans, Black Caribbeans, Chinese and Whites in England from 2001 to 2007. We analysed 134,302 haematological cancer registrations with ethnicity obtained by linkage to the Hospital Episodes Statistics database. Mid-year population estimates from 2001 to 2007 were used. Incidence rate ratios adjusted for age, sex and income were calculated, comparing the six ethnic groups to Whites and to each other. Whites had the highest rates for most subtypes. However, Blacks experienced more than double the incidence of plasma cell and mature T-cell neoplasms compared to other ethnic groups. There were also significant differences in incidence between Indians, Pakistanis and Bangladeshis for Hodgkin lymphoma and mature B-cell neoplasms and between Black African and Black Caribbeans for mature B-cell and other lymphoid neoplasms (all P < 0.001). Our results show that the risk of haematological cancers varies greatly by ethnic group, including within those groups that have traditionally been grouped together (South Asians and Blacks) with many of these differences not explicable by known risk factors. PMID:24033296

  4. The influence of administering "effective microorganisms" to pullets on chosen haematological and biochemical blood indexes.

    PubMed

    Sokó?, R; Michalczyk, M; Spodniewska, A; Barski, D

    2009-01-01

    "Effective Microorganisms" (EM)--a mixture of lactic acid bacteria, photosynthetic bacteria, yeasts and fungi are used mainly in agriculture and organic waste treatment. Recently, they have also been added to water and feed for animals, as well as to processing their excrements into compost and to eliminate the stench. The objective of the present study was to assess the influence of a 14-day administration of an EM solution in drinking water to layer hens on chosen haematological and biochemical indexes. The research was carried out on 120 hens divided into two equal groups. The birds in the experimental group were given drinking water with dissolved EM (5% solution), and those in the control group--water without the preparation. On the 64th day of the aviculture, the hens were weighted and their blood was taken from the wing vein for haematological and biochemical examinations. Administering EM with water to hens did not influence significantly their body weight nor chosen haematological and biochemical indexes. A significant increase was found only in the number of platelets, the level of albumins, the content of total cholesterol and the LDH activity, however, a decrease in the ALT activity was observed. PMID:20169927

  5. Haematological analysis conducted at the departure of the Tour de France 2001.

    PubMed

    Robinson, N; Schattenberg, L; Zorzoli, M; Mangin, P; Saugy, M

    2005-04-01

    Since the introduction of blood analysis performed before major cycling events in 1997, there have been discussions concerning the quality of the results. The aim of our study therefore was to measure blood samples and compare the results obtained on the field with those obtained in the laboratory. For this it was necessary to have blood samples analysed with different instruments to determine the exactness of the results and evaluate the performances of these instruments in order to validate the haematological testing used to reveal athletes abusing recombinant erythropoietin. We report on the haematological analysis of 177 professional cyclists who took part in the Tour de France 2001. All the blood samples were withdrawn in the morning between 7 and 9 am in Dunkerque (France) and were analysed immediately with a transportable analyser. Then the samples were flown to Lausanne (Switzerland) and were reanalysed in two independent ISO 17 025 accredited laboratories with three different analysers. The results confirmed that the most effective haematological follow-up should be performed under standardized pre-analytical conditions and with identical analysers of the same manufacturer to avoid too many variations notably on the haematocrit level and the reticulocyte count. Furthermore, this study suggests that analyses performed on the site are good and could enable the federations to perform a urinary test to detect rhEPO abuse right after the blood analysis. This time saving is essential to fight efficiently recombinant erythropoietin doping, because the half life of the hormone is very short. PMID:15776340

  6. Fertility preservation in patients with haematological disorders: a retrospective cohort study.

    PubMed

    Senapati, Suneeta; Morse, Christopher B; Sammel, Mary D; Kim, Jayeon; Mersereau, Jennifer E; Efymow, Brenda; Gracia, Clarisa R

    2014-01-01

    This study investigated the factors associated with utilization of fertility preservation and the differences in treatments and outcomes by prior chemotherapy exposure in patients with haematological diseases. This study included all 67 women with haematological diseases seen for fertility preservation consultation at two university hospitals between 2006 and 2011. Of the total, 49% had lymphoma, 33% had leukaemia, 7% had myelodysplastic syndrome and 4% had aplastic anaemia; 46% had prior chemotherapy; and 33% were planning for bone marrow transplantation, 33% pursued ovarian stimulation and 7% used ovarian tissue banking; and 48% of patients did not pursue fertility preservation treatment. All five cycle cancellations were in the post-chemotherapy group: three patients with leukaemia and two with lymphoma. Patients with prior chemotherapy had lower baseline antral follicle count (10 versus 22) and received more gonadotrophins to achieve similar peak oestradiol concentrations, with no difference in oocyte yield (10.5 versus 10) after adjustment for age. Embryo yield was similar between those who had prior chemotherapy and those who had not. Half of the patients with haematological diseases who present for fertility preservation have been exposed to chemotherapy. While ovarian reserve is likely impaired in this group, oocyte yield may be acceptable. PMID:24140311

  7. Saprochaete capitata as an emerging fungus among patients with haematological malignencies.

    PubMed

    Ulu-Kilic, Aysegul; Atalay, Mustafa Altay; Metan, Gökhan; Cevahir, Fatma; Koç, Nedret; Eser, Bülent; Çetin, Mustafa; Kaynar, Leyla; Alp, Emine

    2015-08-01

    Saprochaete capitata is a very rare pathogen that causes invasive disease particularly in patients with haematological malignancies. We recognised a clustering of S. capitata fungaemia in recent years. So, we report our 6-year surveillance study of fungaemia among patients with haematological malignancies and haematopoietic stem cell transplant. We performed a retrospective and observational study. Hospitalised patients aged >18 years with haematological malignancies were included in the study. A total of 51 fungaemia episodes of 47 patients were analysed. The characteristics of fungaemia in patients with S. capitata compared to patients with candidemia. Median duration of neutropenia was 21.5 days in patients with S. capitata fungaemia, whereas this duration was significantly shorter in patients with candidemia (8 days). Interval between first and last positive culture was significantly longer in patients with S. capitata fungaemia (P < 0.05). Previous use of caspofungin was significantly more common in patients with S. capitata fungaemia. Thirty-day mortality was found 40% for patients with candidemia, whereas it was 39% for patients with S. capitata. In conclusion, despite its limitations this study showed that a novel and more resistant yeast-like pathogen become prevalent due to use of caspofungin in patients with long-lasting neutropenia which was the most noteworthy finding of this 6-year surveillance study. PMID:26155743

  8. The impact of co-infections on the haematological profile of East African Short-horn Zebu calves.

    PubMed

    Van Wyk, Ilana Conradie; Goddard, Amelia; de C Bronsvoort, B Mark; Coetzer, Jacobus A W; Handel, Ian G; Hanotte, Olivier; Jennings, Amy; Lesosky, Maia; Kiara, Henry; Thumbi, Sam M; Toye, Phil; Woolhouse, Mark W; Penzhorn, Banie L

    2014-03-01

    The cumulative effect of co-infections between pathogen pairs on the haematological response of East African Short-horn Zebu calves is described. Using a longitudinal study design a stratified clustered random sample of newborn calves were recruited into the Infectious Diseases of East African Livestock (IDEAL) study and monitored at 5-weekly intervals until 51 weeks of age. At each visit samples were collected and analysed to determine the infection status of each calf as well as their haematological response. The haematological parameters investigated included packed cell volume (PCV), white blood cell count (WBC) and platelet count (Plt). The pathogens of interest included tick-borne protozoa and rickettsias, trypanosomes and intestinal parasites. Generalized additive mixed-effect models were used to model the infectious status of pathogens against each haematological parameter, including significant interactions between pathogens. These models were further used to predict the cumulative effect of co-infecting pathogen pairs on each haematological parameter. The most significant decrease in PCV was found with co-infections of trypanosomes and strongyles. Strongyle infections also resulted in a significant decrease in WBC at a high infectious load. Trypanosomes were the major cause of thrombocytopenia. Platelet counts were also affected by interactions between tick-borne pathogens. Interactions between concomitant pathogens were found to complicate the prognosis and clinical presentation of infected calves and should be taken into consideration in any study that investigates disease under field conditions. PMID:24553080

  9. Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis

    PubMed Central

    2013-01-01

    Background Hepatitis E virus (HEV) has been described as an emerging pathogen in Brazil and seems to be widely disseminated among swine herds. An autochthonous human case of acute hepatitis E was recently reported. To obtain a better understanding of the phenotypic profiles of both human and swine HEV strains, a experimental study was conducted using the animal model, Macaca fascicularis. Methods Six cynomolgus monkeys (Macaca fascicularis) were inoculated intravenously with swine HEV genotype 3 that was isolated from naturally and experimentally infected pigs in Brazil and the Netherlands. Two other monkeys were inoculated with HEV genotype 3 that was recovered from Brazilian and Argentinean patients with locally acquired acute and fulminant hepatitis E. The haematological, biochemical, and virological parameters of all animals were monitored for 67 days. Results Subclinical hepatitis was observed in all monkeys after inoculation with HEV genotype 3 that was recovered from the infected swine and human patients. HEV RNA was detected in the serum and/or faeces of 6 out of the 8 cynomolgus monkeys between 5 and 53 days after inoculation. The mild inflammation of liver tissues and elevations of discrete liver enzymes were observed. Seroconversions to anti-HEV IgM and/or IgG were detected in 7 animals. Reactivities to anti-HEV IgA were also detected in the salivary samples of 3 animals. Interestingly, all of the infected monkeys showed severe lymphopenia and a trend toward monocytosis, which coincided with elevations in alanine aminotransferase and antibody titres. Conclusions The ability of HEV to cross the species barrier was confirmed for both the swine (Brazilian and Dutch) and human (Argentinean) strains, thus reinforcing the zoonotic risk of hepatitis E in South America. Cynomolgus monkeys that were infected with HEV genotype 3 developed subclinical hepatitis that was associated with haematological changes. Haematological approaches should be considered in future studies of HEV infection. PMID:24148233

  10. Physiological Response of Rabbit Bucks to Dietary Fumonisin: Performance, Haematology and Serum Biochemistry

    Microsoft Academic Search

    Emmanuel O. Ewuola; Francis A. Gbore; Jacob T. Ogunlade; Ranajit Bandyopadhyay; John Niezen; Gabriel N. Egbunike

    2008-01-01

    Maize grains contaminated with fumonisin, a metabolite of Fusarium\\u000a verticillioides was incorporated into matured male rabbits’ diet to evaluate its effects on performance, haematology and serum biochemistry\\u000a in rabbits. Thirty individually caged crossbred adult rabbit bucks averaging 1.36 ± 0.01 kg (about 22–24-week-old) were randomly\\u000a allotted to three treatment diets comprising a control diet (containing 0.35 ± 0.02 mg fumonisin\\/kg) and two test diets containing\\u000a 12.30 ± 0.16 and

  11. INFLUENCE OF D-Nil plus (A POLYHERBAL DRUG) ON HAEMATOLOGICAL AND BIOCHEMICAL CHANGES IN DIABETIC INDUCED RATS

    PubMed Central

    Vanithamani, J.; Selvi, V.; Krishnaswamy, B. G.

    2006-01-01

    Diabetes mellitus, a metabolic disorder, is characterized by hyperglycemia and altered metabolism. The administration of D-Nil plus (a polyherbal drug) showed effective treatment for alloxan induced diabetes in rats. In diabetic rats, haematological profiles namely RBC, WBC, platlet count and haemoglobin were decreased whereas ESR was increased. Similarly biochemical parameters creatinine, urea and protein were decreased but cholesterol level was increased. After the treatment with D-Nil plus, haematological parameters and biochemical parameters were reversed. The results suggest that the D-Nil plus can be used for the treatment of diabetes. PMID:22557203

  12. Effect of artesunate plus sulfadoxine-pyrimethamine on haematological recovery and anaemia, in Kenyan children with uncomplicated, Plasmodium falciparum malaria.

    PubMed

    Obonyo, C O; Taylor, W; Ekvall, H; Kaneko, A; Ter Kuile, F; Olliaro, P; Bjorkman, A; Oloo, A J

    2007-06-01

    Malaria-associated anaemia is a major public-health problem. Although the treatment of uncomplicated, Plasmodium falciparum malaria aims to clear the parasites, relieve the symptoms and permit haematological recovery, data on the impact of antimalarial treatment on haematological recovery are few. Haematological recovery and the prevalence of anaemia were therefore evaluated in 600 Kenyan children with uncomplicated malaria who were randomly assigned to one of three treatment groups. The children were given sulfadoxine-pyrimethamine (SP) on day 0, SP plus artesunate on day 0 (AS1), or SP on day 0 and artesunate on each of days 0-2 (AS3). Haemoglobin (Hb) concentrations were measured on days 0, 7, 14, 21 and 28, with haematological recovery defined as a day-28 Hb concentration of at least 11 g/dl. Only 96 (18%) of the 543 children who were anaemic (i.e. with <11.0 g Hb/dl) at enrolment achieved haematological recovery. The prevalence of anaemia fell from 91% on day 0 to 74% (252/340) by day 28 (P=0.065). Compared with SP alone, neither artesunate regimen resulted in higher Hb concentrations on day 28 (with means of 10.2, 9.9 and 10.2 g/dl for AS3, AS1 and SP, respectively; P=0.254), a higher frequency of haematological recovery (19%, 14% and 20% for AS3, AS1 and SP, respectively; P=0.301) or a greater reduction in the prevalence of anaemia (prevalences in the AS3, AS1 and SP arms falling from 90%, 89% and 93%, respectively, on day 0, to corresponding values of 71%, 82% and 69% on day 28; P=0.40). In fact, between days 0 and 7, the children in the AS3 arm showed a larger drop in mean Hb than the children in the other two treatment arms. In general, haematological recovery was most likely in older children who had mild anaemia at presentation and were parasitologically cured. Overall, the frequencies of haematological recovery were modest and not influenced by the artesunate treatments. Other factors contributing to anaemia need to be explored more fully. PMID:17524243

  13. Prospective study of hepatic, renal, and haematological surveillance in hazardous materials firefighters

    PubMed Central

    Kales, S; Polyhronopoulos, G; Aldrich, J; Mendoza, P; Suh, J; Christiani, D

    2001-01-01

    OBJECTIVES—To evaluate possible health effects related to work with hazardous materials as measured by end organ effect markers in a large cohort over about 2 years, and in a subcohort over 5 years.?METHODS—Hepatic, renal, and haematological variables were analysed from 1996-98 in hazardous materials firefighters including 288 hazardous materials technicians (81%) and 68 support workers (19%). The same end organ effect markers in a subcohort of the technicians were also analysed (n=35) from 1993-98. Support workers were considered as controls because they are also firefighters, but had a low potential exposure to hazardous materials.?RESULTS—During the study period, no serious injuries or exposures were reported. For the end organ effect markers studied, no significant differences were found between technicians and support workers at either year 1 or year 3. After adjustment for a change in laboratory, no significant longitudinal changes were found within groups for any of the markers except for creatinine which decreased for both technicians (p<0.001) and controls (p<0.01).?CONCLUSIONS—Health effects related to work are infrequent among hazardous materials technicians. Haematological, hepatic, and renal testing is not required on an annual basis and has limited use in detecting health effects in hazardous materials technicians.???Keywords: hazardous materials; firefighters; medical surveillance PMID:11160986

  14. Reporting unit size and measurement uncertainty: current Australian practice in clinical chemistry and haematology.

    PubMed

    Hawkins, Robert C; Badrick, Tony

    2015-08-01

    In this study we aimed to compare the reporting unit size used by Australian laboratories for routine chemistry and haematology tests to the unit size used by learned authorities and in standard laboratory textbooks and to the justified unit size based on measurement uncertainty (MU) estimates from quality assurance program data.MU was determined from Royal College of Pathologists of Australasia (RCPA) - Australasian Association of Clinical Biochemists (AACB) and RCPA Haematology Quality Assurance Program survey reports. The reporting unit size implicitly suggested in authoritative textbooks, the RCPA Manual, and the General Serum Chemistry program itself was noted. We also used published data on Australian laboratory practices.The best performing laboratories could justify their chemistry unit size for 55% of analytes while comparable figures for the 50% and 90% laboratories were 14% and 8%, respectively. Reporting unit size was justifiable for all laboratories for red cell count, >50% for haemoglobin but only the top 10% for haematocrit. Few, if any, could justify their mean cell volume (MCV) and mean cell haemoglobin concentration (MCHC) reporting unit sizes.The reporting unit size used by many laboratories is not justified by present analytical performance. Using MU estimates to determine the reporting interval for quantitative laboratory results ensures reporting practices match local analytical performance and recognises the inherent error of the measurement process. PMID:26126036

  15. A panel of cancer-testis genes exhibiting broad-spectrum expression in haematological malignancies

    PubMed Central

    Liggins, Amanda P.; Lim, Seah H.; Soilleux, Elizabeth J.; Pulford, Karen

    2010-01-01

    Cancer-testis (CT) antigens/genes show restricted expression in normal tissues but widespread expression in many tumour types. This, coupled with their ability to induce cytotoxic T-lymphocyte responses, makes them attractive vaccine candidates. Following our identification of PASD1, we have used RT-PCR to analyse the mRNA expression profile of a large panel of CT genes in cell lines derived from haematological malignancies, and have studied Sp17 protein expression in the same cell lines and diffuse large B-cell lymphoma (DLBCL) biopsies. Our extensive analysis revealed multiple CT transcripts exhibiting widespread expression across cell lines derived from 21 B- and 4 T-cell malignancies. The broadest mRNA expression profiles were observed for the following eight CT genes: Sp17 (25/25), PRAME (25/25), CSAGE (24/25), PASD1 (22/25), CAGE/DDX53 (19/25), CTAGE1 (19/25), HAGE/DDX43 (16/25) and PLU-1/JARID1B (15/25). Cell lines derived from more aggressive lymphoma subtypes generally expressed CT transcripts at higher frequency. Sp17 protein was detected in a number of cell lines and in six of eleven (54.5%) DLBCL biopsies. Analysis of Sp17 protein expression, by immunohistochemistry and Western blotting, broadens the scope of this CT antigen as a potentially relevant clinical target in haematological malignancies. Further studies of protein expression are now needed to validate these antigens as vaccine candidates. PMID:20726502

  16. Age-related changes in the haematology of female F344 rats.

    PubMed

    Turton, J A; Hawkey, C M; Hart, M G; Gwynne, J; Hicks, R M

    1989-10-01

    As little comprehensive baseline data are available on age-related haematological changes in genetically-defined rat strains, the haematology of female F344 rats is described in animals sampled at 2, 4, 8, 20, 66 and 121 weeks of age. Values for Hb, RBC and PCV increased from 2 weeks of age to reach adult levels at 8 weeks, whereas MCV, MCH and reticulocyte counts were high initially but decreased to reach the adult range at 8 weeks. Between 66 and 121 weeks, reticulocyte counts were significantly increased and values for MCHC significantly decreased. Lymphocytes were the predominant white cell type in each age group. The absolute numbers of neutrophils and lymphocytes showed slight variations between 2 and 66 weeks and both cell types increased significantly between 66 and 121 weeks. Platelet counts showed no overall age-related trends. Fibrinogen values increased from 2 weeks of age to reach the adult level at 8 weeks. One animal of the 14 sampled at 121 weeks showed changes in the blood, liver and spleen consistent with a diagnosis of lymphoid leukaemia. PMID:2811267

  17. Bacillus spp. among hospitalized patients with haematological malignancies: clinical features, epidemics and outcomes.

    PubMed

    Ozkocaman, V; Ozcelik, T; Ali, R; Ozkalemkas, F; Ozkan, A; Ozakin, C; Akalin, H; Ursavas, A; Coskun, F; Ener, B; Tunali, A

    2006-10-01

    Between April 2000 and May 2005, 350 bacteraemic episodes occurred among patients treated in our haematology unit. Two hundred and twenty-eight of these episodes were caused by Gram-positive pathogens, most commonly coagulase-negative staphylococci and Staphylococcus aureus. One hundred and twenty-two episodes were due to Gram-negative pathogens, with a predominance of Escherichia coli, Acinetobacter baumannii and Pseudomonas aeruginosa. Bacillus bacteraemias constituted 12 of these episodes occurring in 12 patients, and accounted for 3.4% of all bacteraemic episodes. Of the 12 strains evaluated, seven were Bacillus licheniformis, three were Bacillus cereus and two were Bacillus pumilus. Seven episodes presented with bloodstream infection, three with pneumonia, one with severe abdominal pain and deterioration of liver function, and one with a catheter-related bloodstream infection. B. licheniformis was isolated from five patients who had been hospitalized at the same time. This outbreak was related to non-sterile cotton wool used during skin disinfection. B. cereus and B. licheniformis isolates were susceptible to cefepime, carbapenems, aminoglycosides and vancomycin, but B. pumilus isolates were resistant to all antibiotics except for quinolones and vancomycin. Two deaths were observed. In conclusion, Bacillus spp. may cause serious infections, diagnostic and therapeutic dilemmas, and high morbidity and mortality in patients with haematological malignancies. Both B. cereus and B. licheniformis may be among the 'new' Gram-positive pathogens to cause serious infection in patients with neutropenia. PMID:16891037

  18. Haematological malignancies in childhood in Croatia: Investigating the theories of depleted uranium, chemical plant damage and ‘population mixing’

    Microsoft Academic Search

    B. Labar; I. Rudan; D. Ivankovic; Z. Biloglav; M. Mrsic; M. Strnad; A. Fucic; A. Znaor; T. Bradic; H. Campbell

    2004-01-01

    Some of potential causes proposed to explain the reported increase of haematological malignancies in childhood during or after the war period in several countries include depleted uranium, chemical pollution and population mixing theory. The aim of this study was to define the population of Croatian children aged 0–14 years who were potentially exposed to each of those risks during the

  19. Effects of Oral Exposure of Acrylamide on Plasma Levels of Thyroid Hormones and Haematological Parameters in the Swiss Albino Mice

    Microsoft Academic Search

    Asha Sharma; Jyotsna Jain

    Effects of acrylamide on thyroid hormone levels and haematological parameters in Swiss albino mice were investigated by exposing them with 3 dose levels set below LD50. Mortality in animals was nil with a significant decrease in the mean body weight gain. The various toxic morphological effects noted were sluggish appearance, bizarre behaviour, stunted growth, reduced limb movements, diminished food and

  20. Allogeneic and autologous transplantation for haematological diseases, solid tumours and immune disorders: current practice in Europe 2009

    Microsoft Academic Search

    P. Ljungman; M. Bregni; M. Brune; J. Cornelissen; T. J. M. de Witte; G. Dini; H. Einsele; H. B. Gaspar; A. Gratwohl; J. Passweg; C. Peters; V. Rocha; R. Saccardi; H. Schouten; A. Sureda; A. Tichelli; A. Velardi; D. Niederwieser

    2010-01-01

    The European Group for Blood and Marrow Transplantation regularly publishes special reports on the current practice of haematopoietic SCT for haematological diseases, solid tumours and immune disorders in Europe. Major changes have occurred since the first report was published. HSCT today includes grafting with allogeneic and autologous stem cells derived from BM, peripheral blood and cord blood. With reduced-intensity conditioning

  1. Lamivudine treatment failure in preventing fatal outcome of de novo severe acute hepatitis B in patients with haematological diseases

    Microsoft Academic Search

    Enzo Petrelli; Maria Balducci; Carlo Pieretti; Marco B. Luigi Rocchi; Massimo Clementi; Aldo Manzin

    2001-01-01

    Background: Patients with malignant haematological diseases administered or no longer receiving immunosuppressive therapy are at high risk of reactivation or de novo hepatitis B infection and fulminant hepatitis. Despite promising results in the treatment of chronic hepatitis and its use in selected patients with acute hepatitis B, there is no consensus on lamivudine treatment in severe acute hepatitis portending a

  2. Haematological and biochemical changes in experimental Trypanosoma evansi infection in rabbits.

    PubMed

    Sivajothi, S; Rayulu, V C; Sudhakara Reddy, B

    2015-06-01

    New Zealand white rabbits (N = 4) were challenged with the local strain of Trypanosoma evansi. Each rabbit was infected with 5 × 10(5) trypanosomes subcutaneously. The infection was characterized by intermittent pyrexia, undulating parasitaemia, anorexia and emaciation. The infected rabbits were examined daily for development of clinical signs and infection status by wet blood-films made from the ear veins. Thick and thin blood smears were also examined daily until the end of the experiment for description of blood cells. Differential leukocyte count (DLC) was also done. The parasite was observed in the blood during the acute phase only. Leukocytosis in the acute phase followed by leukopenia during the chronic phase was recognized. Haematological studies revealed reduced TEC, Hb and PCV. The main changes in the erythrocytes were macrocytes, hypochromic cells, Howell-Jolly bodies, target cells, stomatocytes and burr cells. Serum chemistry revealed hypoproteinemia, hypocholesterolaemia, hypoglycemia, hyperbilirubinemia, elevated creatinine, BUN, increased AST and ALT. PMID:26064003

  3. Primary immunodeficiencies predisposed to Epstein-Barr virus-driven haematological diseases.

    PubMed

    Parvaneh, Nima; Filipovich, Alexandra H; Borkhardt, Arndt

    2013-09-01

    Epstein-Barr virus (EBV), a ubiquitous human herpesvirus, maintains lifelong subclinical persistent infections in humans. In the circulation, EBV primarily infects the B cells, and protective immunity is mediated by EBV-specific cytotoxic T cells (CTLs) and natural killer (NK) cells. However, EBV has been linked to several devastating diseases, such as haemophagocytic lymphohistiocytosis (HLH) and lymphoproliferative diseases in the immunocompromised host. Some types of primary immunodeficiencies (PIDs) are characterized by the development of EBV-associated complications as their predominant clinical feature. The study of such genetic diseases presents an ideal opportunity for a better understanding of the biology of the immune responses against EBV. Here, we summarize the range of PIDs that are predisposed to EBV-associated haematological diseases, describing their clinical picture and pathogenetic mechanisms. PMID:23758097

  4. Haematology and serum biochemistry of golden eagle ( Aquila chrysaetos) in Iran

    Microsoft Academic Search

    S. Nazifi; A. Nabinejad; M. Sepehrimanesh; S. L. Poorbaghi; F. Farshneshani; M. Rahsepar

    2008-01-01

    Haematological and serum biochemical values were estimated in blood samples collected from 21 apparently adult golden eagles\\u000a (Aquila chrysaetos) of both sexes. The mean values of red blood cells, packed cell volume, haemoglobin, white blood cells, heterophils, lymphocytes,\\u000a monocytes and eosinophils were 1.63?±?0.11?×?1012\\/l, 0.47?±?0.009 l\\/l, 91.73?±?1.52 g\\/l, 24.31?±?1.97?×?109\\/l, 4.40?±?0.22?×?109\\/l, 16.81?±?0.65?×?109\\/l, 0.99?±?0.19?×?109\\/l and 2.10?±?0.30?×?109\\/l, respectively. The leucocytes had 69.14%, 4.09%, 18.12% and 8.65%

  5. Tales from the Jazz ASH: highlights from the 2013 American Society of Haematology meeting

    PubMed Central

    Mazzarella, Luca

    2014-01-01

    The 55th annual ASH meeting was held in pleasant New Orleans and was the largest in its history, with 22,495 participants coming from 113 nations. A ‘bench-to-bedside and back’ attitude characterises haematology probably more than any other discipline in medicine and, as usual, this was reflected in the extremely wide breadth of the topics covered, including the last results from clinical trials and cutting-edge advancements in basic science. This year, the balance was arguably skewed: few truly clinical practice-changing results were presented. On the other hand, a great number of basic and translational studies significantly increased our understanding of the biology of numerous malignancies and heralded the coming of age of disruptive technologies. Namely, above all, next generation sequencing and T cell engineering-based cell therapy. PMID:24678345

  6. First-dose pharmacokinetics of aminoglycosides in critically ill haematological malignancy patients.

    PubMed

    Blackburn, Laura M; Tverdek, Frank P; Hernandez, Mike; Bruno, Jeffrey J

    2015-01-01

    The primary objective of this study was to determine the volume of distribution (Vd) (L/kg) of intravenous aminoglycosides (AGs) in critically ill haematological malignancy patients. Secondary objectives were to determine the body weight (actual, ideal, adjusted or lean) that yields the most precise estimate of Vd when normalised in L/kg as well as the frequency that current first-dose strategies result in post-distributional peak concentrations (C(peak)) within the target range (tobramycin 16-24 mg/L; amikacin 32-48 mg/L). In total, 58 AG doses were included (tobramycin, n = 34; amikacin, n = 24). Median Vd was 0.38 L/kg normalised per the most precise dose weight, which was actual body weight (ABW). The median dose was 445 mg (5.8 mg/kg ABW) for tobramycin and 1200 mg (13.8 mg/kg ABW) for amikacin. Target C(peak) (tobramycin 20mg/L; amikacin 40 mg/L) was achieved in only 25% of all AG episodes, with 4% exceeding the target and 71% falling below the target. Twenty-four organisms were isolated in the study sample; target C(peak) achievement (tobramycin 20 mg/L; amikacin 40 mg/L) would yield a peak:minimum inhibitory concentration of 10 in 75% and 52% of organisms, respectively. In conclusion, an increased Vd of AGs was identified in this critically ill haematological malignancy patient sample, and current dosing yielded a suboptimal C(peak) in the majority of patients. PMID:25455848

  7. Haematology and Serum Biochemistry Parameters and Variations in the Eurasian Beaver (Castor fiber)

    PubMed Central

    Girling, Simon J.; Campbell-Palmer, Roisin; Pizzi, Romain; Fraser, Mary A.; Cracknell, Jonathan; Arnemo, Jon; Rosell, Frank

    2015-01-01

    Haematology parameters (N = 24) and serum biochemistry parameters (N = 35) were determined for wild Eurasian beavers (Castor fiber), between 6 months – 12 years old. Of the population tested in this study, N = 18 Eurasian beavers were from Norway and N = 17 originating from Bavaria but now living extensively in a reserve in England. All blood samples were collected from beavers via the ventral tail vein. All beavers were chemically restrained using inhalant isoflurane in 100% oxygen prior to blood sampling. Results were determined for haematological and serum biochemical parameters for the species and were compared between the two different populations with differences in means estimated and significant differences being noted. Standard blood parameters for the Eurasian beaver were determined and their ranges characterised using percentiles. Whilst the majority of blood parameters between the two populations showed no significant variation, haemoglobin, packed cell volume, mean cell haemoglobin and white blood cell counts showed significantly greater values (p<0.01) in the Bavarian origin population than the Norwegian; neutrophil counts, alpha 2 globulins, cholesterol, sodium: potassium ratios and phosphorus levels showed significantly (p<0.05) greater values in Bavarian versus Norwegian; and potassium, bile acids, gamma globulins, urea, creatinine and total calcium values levels showed significantly (p<0.05) greater values in Norwegian versus Bavarian relict populations. No significant differences were noted between male and female beavers or between sexually immature (<3 years old) and sexually mature (?3 years old) beavers in the animals sampled. With Eurasian beaver reintroduction encouraged by legislation throughout Europe, knowledge of baseline blood values for the species and any variations therein is essential when assessing their health and welfare and the success or failure of any reintroduction program. This is the first study to produce base-line blood values and their variations for the Eurasian beaver. PMID:26066344

  8. The Effect of Cement Dust Exposure on Haematological Parameters of Cement Factory workers in Nalagonda, Andhra Pradesh.

    NASA Astrophysics Data System (ADS)

    Guguloth, Mohan Rao.; Sambanaik, A.; srinivasnaik, L.; Mude, Jagadishnaik.

    2012-10-01

    This study was measured on haematological parameters in workers exposed to cement dust in order to test the the hypothesis and to identify a simple, readily available, cost effective screening test that could help in identifying the presence of disease, its severity, that Cement dust exposure may perturb these functions related to their workplace.Assesment of haematological parameters were performed in 100exposed workers occupationally exposed to cement dust and 50 matched unexposed controls with ages ranging from 20-35, 35-50, 50-65 years. The blood samples were taken from them and percentage of hemoglobin, Lymphocytes / monocytes count were analysed.The hemoglobin percentage of exposed workers were significantly lower(P<0.05).Lymphocytes/Monocytes counts of exposed workers was insignificant (P<0.05).These results suggest that long term occupational exposure to cement dust may perturb haemopoietic function.

  9. Epidemiology of invasive fungal diseases among patients with haematological disorders in the Asia-Pacific: a prospective observational study.

    PubMed

    Hsu, L Y; Lee, D G; Yeh, S P; Bhurani, D; Khanh, B Q; Low, C Y; Norasetthada, L; Chan, T; Kwong, Y L; Vaid, A K; Alejandria, I; Mendoza, M; Chen, C Y; Johnson, A; Tan, T Y

    2015-06-01

    We conducted a 2-year multicentre prospective observational study to determine the epidemiology of and mortality associated with invasive fungal diseases (IFDs) among patients with haematological disorders in Asia. Eleven institutions from 8 countries/regions participated, with 412 subjects (28.2% possible, 38.3% probable and 33.5% proven IFDs) recruited. The epidemiology of IFDs in participating institutions was similar to Western centres, with Aspergillus spp. (65.9%) or Candida spp. (26.7%) causing the majority of probable and proven IFDs. The overall 30-day mortality was 22.1%. Progressive haematological disorder (odds ratio [OR] 5.192), invasive candidiasis (OR 3.679), and chronic renal disease (OR 6.677) were independently associated with mortality. PMID:25749561

  10. Haematological Safety of Perinatal Zidovudine in Pregnant HIV-1–Infected Women in Thailand: Secondary Analysis of a Randomized Trial

    PubMed Central

    Briand, Nelly; Lallemant, Marc; Jourdain, Gonzague; Techapalokul, Somnuek; Tunthanathip, Preecha; Suphanich, Surachet; Chanpoo, Truengta; Traisathit, Patrinee; McIntosh, Kenneth; Coeur, Sophie Le

    2007-01-01

    Objectives: To respond to the primary safety objective of the Perinatal HIV Prevention Trial 1 (PHPT-1) by studying the evolution of haematological parameters according to zidovudine exposure duration in HIV-1?infected pregnant women. Design: Multicenter, randomized, double-blind, controlled trial of different durations of zidovudine prophylaxis. Setting: 27 hospitals in Thailand. Participants: 1,436 HIV-infected pregnant women in PHPT-1. Intervention: Zidovudine prophylaxis initiation at 28 or 35 wk gestation. Outcome measures: Haemoglobin level, leucocytes, total lymphocyte counts, and absolute neutrophil counts were measured at 26, 32, and 35 wk and at delivery. The evolution of haematological parameters was estimated between 26 and 35 wk (zidovudine/placebo) and between 35 wk and delivery to compare a long versus short zidovudine exposure. For each parameter, linear mixed models were adjusted on baseline sociodemographic variables, HIV clinical stage, CD4 count, and viral load. Results: Between 26 and 35 wk, haemoglobin, leucocytes, and absolute neutrophil counts decreased in zidovudine-exposed compared to unexposed women (mean difference [95% CI] ?0.4 [?0.5 to ?0.3], ?423 [?703 to ?142], ?485 [?757 to ?213], respectively). However, between 35 wk and delivery, the haematological parameters increased faster in women exposed to long rather than short durations of zidovudine (0.1 [0.0 to 0.1]; 105 [18 to 191]; 147 [59 to 234], respectively). At delivery, the differences were not statistically significant, except for mean haemoglobin level, which remained slightly lower in the long zidovudine treatment group (difference: 0.2 g/dl). Zidovudine had no negative impact on the absolute lymphocyte counts. Conclusion: Zidovudine initiated at 28 wk gestation rather than 35 wk had a transient negative impact on the evolution of haematological parameters, which was largely reversed by delivery despite continuation of zidovudine. This result provides reassurance about the safety of early initiation of zidovudine prophylaxis during pregnancy to maximize prevention of perinatal HIV. PMID:17476315

  11. Iron requirements based upon iron absorption tests are poorly predicted by haematological indices in patients with inactive inflammatory bowel disease

    PubMed Central

    Lomer, Miranda CE; Cook, William B; Jan-Mohamed, Hamid Jan B; Hutchinson, Carol; Liu, Ding Yong; Hider, Robert C; Powell, Jonathan J

    2012-01-01

    Iron deficiency (ID) and iron deficiency anaemia (IDA) are common in patients with inflammatory bowel disease (IBD). Traditional clinical markers of iron status can be skewed in the presence of inflammation meaning that a patient’s iron status can be misinterpreted. Additionally, iron absorption is known to be down-regulated in patients with active IBD. However, whether this is the case for quiescent or mildly active disease has not been formally assessed. This study aimed to investigate the relationship between iron absorption, iron requirements and standard haematological indices in IBD patients without active disease. Twenty nine patients with quiescent or mildly active IBD and 28 control subjects undertook an iron absorption test which measured sequential rises in serum iron over four hours following ingestion of 200 mg ferrous sulphate. At baseline, serum iron, transferrin saturation, non-transferrin bound iron (NTBI), ferritin and soluble transferrin receptor were all measured. Thereafter (30-240 minutes) only serum iron and NTBI were measured. Iron absorption did not differ between the two groups (P=0.9; RM-ANOVA). In control subjects baseline haematological parameters predicted iron absorption (i.e. iron requirements) but this was not the case for patients with IBD. Iron absorption is normal in quiescent or mildly active IBD patients but standard haematological parameters do not accurately predict iron requirements. PMID:22152498

  12. Effect of Aqueous Extract of Telfairia occidentalis Leaf on the Performance and Haematological Indices of Starter Broilers

    PubMed Central

    P. N., Onu

    2012-01-01

    This experiment was conducted to determine the effect of aqueous extract of Telfairia occidentalis (Fluted Pumpkin) leaf on the performance and haematological indices of starter broilers. A total of 200, 8-day-old broiler chicks were randomly allotted to five (5) treatments, each with 4 replicate groups containing 10 chicks and fed with standard starter broiler diets. Telfaria occidentalis leaves extract (FPLE) was added at 0, 40, 80, 120, and 160?mL/litre of drinking water. Growth performance and haematological indices were evaluated. Results showed that there was significant (P < 0.05) difference in weight gain, feed conversion, and protein efficiency ratios of the birds among the treatments. Birds fed 80?ml FPLE/litre of water had significantly the highest weight gain and the best feed conversion and protein efficiency ratios. There was no significant (P > 0.05) variations in the feed and water intakes of the birds. Results also show no significant (P > 0.05) difference in haematological indices of birds among the treatments. The results of this study indicate that, for enhanced weight gain and feed conversion efficiency, birds should be fed 80?mL FPLE/litre of water. PMID:23738128

  13. Outbreak of Multi-Drug Resistant Pseudomonas aeruginosa Bloodstream Infection in the Haematology Unit of a South African Academic Hospital

    PubMed Central

    Mudau, Maanda; Jacobson, Rachael; Minenza, Nadia; Kuonza, Lazarus; Morris, Vida; Engelbrecht, Heather; Nicol, Mark P.; Bamford, Colleen

    2013-01-01

    Objective To describe an outbreak of multi-resistant Pseudomonas aeruginosa bloodstream infections (MRPA-BSI) that occurred in the haematology ward of a tertiary academic hospital in Cape Town, South Africa, and determine risk factors for acquisition of MRPA-BSI. Methods The outbreak investigation included a search for additional cases, review of patient records, environmental and staff screening, molecular typing using pulsed-field gel electrophoresis (PFGE) and Multi-locus sequencing (MLST) and a retrospective case-control study. Results Ten MRPA-BSI cases occurred in the haematology ward between January 2010 and January 2011. The case fatality rate was 80%. Staff screening specimens were negative for MRPA and an environmental source was not identified. PFGE showed that 9/10 isolates were related. MLST showed that 3 of these 9 isolates belonged to Sequence type (ST) 233 while the unrelated isolate belonged to ST260. Conclusion We have described an outbreak of MRPA-BSI occurring over an extended period of time among neutropenic haematology patients. Molecular typing confirms that the outbreak was predominantly due to a single strain. The source of the outbreak was not identified, but the outbreak appears to have been controlled following intensive infection control measures. PMID:23516393

  14. A new frontier in haematology - combining pharmacokinetic with pharmacodynamic factors to improve choice and dose of drug.

    PubMed

    Arpon, David Rey; Gandhi, Maher K; Martin, Jennifer H

    2014-08-01

    The issue of tailored dosing adjusted according to a range of patient-specific factors other than bodyweight or body surface area is of large and increasing clinical and financial concern. Even if it is known that dosing alterations are likely to be required for parameters such as body composition, gender and pharmacogenetics, the amount of dosing change is unknown. Thus, pharmacokinetically guided dosing is making a resurgence, particularly in areas of medicine where there are cost constraints or safety issues, such as in haematology medications. However, the evidence to support the behaviour is minimal, particularly when long-term outcomes are considered. In haematology, there are particular issues around efficacy, toxicity and overall cost. Newer targeted agents, such as the monoclonal antibody rituximab and the tyrosine kinase inhibitor imatinib, whilst clearly being highly effective, are dosed on a milligram per square metre (rituximab) or fixed dose basis (imatinib), regardless of body composition, tumour aspects or comorbidity. This review questions this practice and raises important clinical issues; specifically, the clinical potential for combined pharmacokinetically and pharmacodynamically guided dosing of new targeted agents in haematological malignancies. This pharmacokinetically and pharmacodynamically guided dosing is an emerging area of clinical pharmacology, driven predominantly by toxicity, efficacy and cost issues, but also because reasonable outcomes are being noted with more appropriately dosed older medications adjusted for patient-specific factors. Clinical trials to investigate the optimization of rituximab dose scheduling are required. PMID:24433338

  15. Changes in haematological profile of common pheasant (Phasianus colchicus) induced by transit to pheasantry.

    PubMed

    Voslarova, E; Bedanova, I; Vecerek, V; Pistekova, V; Chloupek, P; Suchy, P

    2006-10-01

    The aim of this study was to assess haematological changes in hand-reared pheasants (Phasianus colchicus) transported from intensive housing facilities to a pheasantry. Selected haematological parameters were monitored in a group of 100 pheasants (50 males and 50 females) aged of 9 weeks that were transported for 4 hours by a covered lorry in crates, with a total body weight of 12 +/- 0.5 kg per crate (Group C12 - floor space: 290 cm2/kg) and with a total body weight of 18 +/- 0.5 kg per crate (Group C18 - floor space: 195 cm2/kg). Blood samples were taken from 10 randomly selected males and 10 females before transport (CON group) and 20 hours after transport (C12 and C18 groups). Examinations consisted in determining the total erythrocyte and leukocyte counts, haematocrit values, haemoglobin levels and differential leukocyte counts, whereby the proportions of heterophil, basophil and eosinophil granulocytes, lymphocytes and monocytes of the total leukocytes were computed. The changes in the parameters of red blood cell count were manifested by an increase (P < 0.01) in the haemoglobin level, MCH (mean cell haemoglobin) and MCHC (mean cell haemoglobin concentration) values and a decrease (P < 0.01) in the total erythrocyte count and haematocrit level in both C12 and C18 pheasants, when compared with the control group of non-transported pheasants. C18 pheasants exhibited also a significant increase (P < 0.05) in MCV (mean cell value) value. When analyzing differential leukocyte counts, C18 pheasants showed a decrease (P < 0.01) in heterophil counts and H/L ratio, whereas values in C12 pheasants did not differ from the non-transported control group. Individual counts of lymphocytes were decreased (P < 0.05) in C12 pheasants, whereas basophil counts were increased (P < 0.01) in both C12 and C18 pheasants. Total leukocyte count was decreased (P < 0.01) in C12 and C18 pheasants. In conclusion, the specific requirements of pheasants, as primarily wild animals, for the density in crates should be respected during transportation and they should be transported at lower densities than other poultry species, at least 290 cm2/kg live weight should be provided. PMID:17078531

  16. Multivitamin supplementation improves haematologic status in children born to HIV-positive women in Tanzania

    PubMed Central

    Liu, Enju; Duggan, Christopher; Manji, Karim P; Kupka, Roland; Aboud, Said; Bosch, Ronald J; Kisenge, Rodrick R; Okuma, James; Fawzi, Wafaie W

    2013-01-01

    Introduction Anaemia is prevalent among children born to HIV-positive women, and it is associated with adverse effects on cognitive and motor development, growth, and increased risks of morbidity and mortality. Objective To examine the effect of daily multivitamin supplementation on haematologic status and mother-to-child transmission (MTCT) of HIV through breastfeeding. Methods A total of 2387 infants born to HIV-positive women from Dar es Salaam, Tanzania were enrolled in a randomized, double-blind, placebo-controlled trial, and provided a daily oral supplement of multivitamins (vitamin B complex, C and E) or placebo at age 6 weeks for 24 months. Among them, 2008 infants provided blood samples and had haemoglobin concentrations measured at baseline and during a follow-up period. Anaemia was defined as haemoglobin concentrations<11 g/dL and severe anaemia<8.5 g/dL. Results Haemoglobin concentrations among children in the treatment group were significantly higher than those in the placebo group at 12 (9.77 vs. 9.64 g/dL, p=0.03), 18 (9.76 vs. 9.57 g/dL, p=0.004), and 24 months (9.93 vs. 9.75 g/dL, p=0.02) of follow-up. Compared to those in the placebo group, children in the treatment group had a 12% lower risk of anaemia (hazard ratio (HR): 0.88; 95% CI: 0.79–0.99; p=0.03). The treatment was associated with a 28% reduced risk of severe anaemia among children born to women without anaemia (HR: 0.72; 95% CI: 0.56–0.92; p=0.008), but not among those born to women with anaemia (HR: 1.10; 95% CI: 0.79–1.54; p=0.57; p for interaction=0.007). One thousand seven hundred fifty three infants who tested HIV-negative at baseline and had HIV testing during follow-up were included in the analysis for MTCT of HIV. No association was found between multivitamin supplements and MTCT of HIV. Conclusions Multivitamin supplements improve haematologic status among children born to HIV-positive women. Further trials focusing on anaemia among HIV-exposed children are warranted in the context of antiretroviral therapy. PMID:23948440

  17. Infection control issues in patients with haematological malignancies in the era of multidrug-resistant bacteria.

    PubMed

    Ruhnke, Markus; Arnold, Renate; Gastmeier, Petra

    2014-12-01

    Drug-resistant Gram-negative and Gram-positive bacteria are now increasingly identified as a cause of infections in immunocompromised hosts. Bacteria identified include the multidrug-resistant (MDR) and even pandrug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa, as well as carbapenem-resistant Enterobacteriaceae spp. The threat from MDR pathogens has been well-documented in the past decade with warnings about the consequences of inappropriate use of antimicrobial drugs. Resistant bacteria can substantially complicate the treatment of infections in critically ill patients and can have a substantial effect on mortality. Inappropriate antimicrobial treatment can affect morbidity, mortality, and overall health-care costs. Evidence-based data for prevention and control of MDR pathogen infections in haematology are scarce. Although not yet established a bundle of infection control and prevention measures with an anti-infective stewardship programme is an important strategy in infection control, diagnosis, and antibiotic selection with optimum regimens to ensure a successful outcome for patients. PMID:25456379

  18. Haematological evaluation of patients bitten by the jararaca, Bothrops jararaca, in Brazil.

    PubMed

    Santoro, Marcelo L; Sano-Martins, Ida S; Fan, Hui W; Cardoso, Joăo L C; Theakston, R David G; Warrell, David A

    2008-06-15

    Complete blood counts are used frequently by physicians to assess and manage the development of complications of diseases. We studied 100 patients bitten by Bothrops jararaca snakes, and correlated their haematological values with the severity of envenoming and the development of complications. Patients who developed both local and systemic bleeding showed a greater drop in packed cell volume, red blood cell (RBC) count and haemoglobin concentration than those with who did not bleed. No morphological changes in RBCs were seen in blood films. Total white blood cell (WBC) counts were significantly higher in the clinically "more severe" group than in the "less severe" group on admission. Neutrophilic leucocytosis with left shift was present on admission, concurrently with a decrease in eosinophil and lymphocyte counts. These changes tend to become more marked 6h after antivenom therapy, and are greatest in "more severe" envenoming. Thrombocytopenia on admission is positively associated with the development of systemic bleeding and the severity of envenoming. Thrombocytopenia may also be a useful prognostic indicator for the development of local complications, such as necrosis. The intensity of neutrophilia and eosinopenia might be used to follow the progression of necrosis in victims of snake bite. PMID:18471839

  19. Atlas of Genetics and Cytogenetics in Oncology and Haematology in 2013

    PubMed Central

    Huret, Jean-Loup; Ahmad, Mohammad; Arsaban, Mélanie; Bernheim, Alain; Cigna, Jérémy; Desangles, François; Guignard, Jean-Christophe; Jacquemot-Perbal, Marie-Christine; Labarussias, Maureen; Leberre, Vanessa; Malo, Anne; Morel-Pair, Catherine; Mossafa, Hossein; Potier, Jean-Claude; Texier, Guillaume; Viguié, Franck; Yau Chun Wan-Senon, Sylvie; Zasadzinski, Alain; Dessen, Philippe

    2013-01-01

    The Atlas of Genetics and Cytogenetics in Oncology and Haematology (http://AtlasGeneticsOncology.org) is a peer-reviewed internet journal/encyclopaedia/database focused on genes implicated in cancer, cytogenetics and clinical entities in cancer and cancer-prone hereditary diseases. The main goal of the Atlas is to provide review articles that describe complementary topics, namely, genes, genetic abnormalities, histopathology, clinical diagnoses and a large iconography. This description, which was historically based on karyotypic abnormalities and in situ hybridization (fluorescence in situ hybridization) techniques, now benefits from comparative genomic hybridization and massive sequencing, uncovering a tremendous amount of genetic rearrangements. As the Atlas combines different types of information (genes, genetic abnormalities, histopathology, clinical diagnoses and external links), its content is currently unique. The Atlas is a cognitive tool for fundamental and clinical research and has developed into an encyclopaedic work. In clinical practice, it contributes to the cytogenetic diagnosis and may guide treatment decision making, particularly regarding rare diseases (because they are numerous and are frequently encountered). Readers as well as the authors of the Atlas are researchers and/or clinicians. PMID:23161685

  20. Automated counting of cells in cerebrospinal fluid using the CellDyn-4000 haematology analyser.

    PubMed

    Hoffmann, Johannes J M L; Janssen, Willy C M

    2002-11-01

    Counting of cells in cerebrospinal fluid is currently performed manually. Because of the inherent analytical and economical disadvantages, we attempted to introduce a fully automated method. Therefore, we validated the Abbott CellDyn-4000 haematology analyser for counting cells in cerebrospinal fluid. The analyser was used in its standard configuration with the simple precaution of a preceding blank sample. As for leukocyte counting the analyser yielded high precision (CV approximately 5% above the upper reference limit), good linearity, low limit of detection (2/microl) and excellent correlation (r > 0.99) with the counting chamber method. The differential leukocyte count was equally accurate and precise, even in the low concentration range. Performance of the erythrocyte count was impaired by its high limit of detection (6/nl) and it appeared satisfactory only for detecting blood admixture due to traumatic puncture. The specificity of the analyser is excellent, since it correctly classified non-viable leukocytes and excluded yeast cells from the leukocyte count in a patient with cryptococcal meningitis. We conclude that the CellDyn-4000 is well suited for quickly and reliably counting leukocytes in cerebrospinal fluid. Developing some software modifications might make the analyser useful also for performing erythrocyte counting in cerebrospinal fluid. PMID:12521237

  1. Chronic diclofenac (DCF) exposure alters both enzymatic and haematological profile of African catfish, Clarias gariepinus.

    PubMed

    Ajima, Malachy N O; Ogo, Ogo A; Audu, Bala S; Ugwoegbu, Kyrian C

    2014-11-01

    Abstract Pharmaceuticals are used extensively in human and veterinary medicine to eradicate or prevent diseases. The residues of these drugs have been detected in aquatic ecosystem; nevertheless, their toxicological effects on Clarias gariepinus have not been critically investigated. In this study, the toxic effects of diclofenac (DCF), a non-steroid anti-inflammatory drug, were studied in C. gariepinus by acute and chronic static renewable bioassay. The 96?h LC50 of DCF to C. gariepinus was 25.12?mg/L. Exposure to acute toxicity resulted in abnormal behavior and mortality of some fish. Compared with the control, chronic exposure of the fish to concentration (1.57, 3.14 and 6.28?mg/L) showed significantly higher mean corpuscular haemoglobin concentration (MCHC), mean corpuscular volume (MCV) and white blood cell (WBC), with significantly lower haemoglobin (Hb), haematocrit, red blood cell (RBC) and mean corpuscular haemoglobin (MCH) with increase in the concentration of the drug. Furthermore, the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and glucose values significantly increased while protein levels were reduced (p?haematological changes in the fish and recommends that these parameters be used as potential biomarkers for assessing residual pharmaceuticals available in aquatic ecosystem. PMID:25367777

  2. Evaluation of the Vega haematology analyser in a university hospital setting.

    PubMed

    Corberand, J X; Segonds, C; Fontanilles, A M; Cambus, J P; Fillola, G; Laharrague, P

    1999-02-01

    The performance of the ABX Vega haematology analyser was compared with that of the Sysmex NE-8000, with specific attention to flagging performance and ergonomics. Eight hundred routine samples underwent precision and interinstrument variability studies and 168 samples corresponding to various blood disorders were studied meanwhile. Results from the two instruments gave excellent correlation (r > 0.900) for most parameters except MCHC (r = 0.114), basophil and monocyte percentages (r = 0.617 and 0.552, respectively). The reproducibility, repeatability, linearity, carry-over and stability of the Vega were satisfactory; 'flagging' occurred in 31% of routine samples with sensitivity 88.8%, specificity 41.3% and positive predictive value 85.7%. Various flags appeared in 91% (42/46) of cases where blast cells were microscopically identified. In the four remaining cases, CBC anomalies would themselves have justified microscopic examination of a smear. On 'CBC only' mode reagent consumption was significantly reduced. In the laboratory the analyser was best appreciated for its user-friendliness. PMID:10197256

  3. Effect of aerobic exercise on premenstrual symptoms, haematological and hormonal parameters in young women.

    PubMed

    El-Lithy, A; El-Mazny, A; Sabbour, A; El-Deeb, A

    2015-05-01

    The objective of this study was to investigate the effect of aerobic exercise on premenstrual symptoms, haematological and hormonal parameters in young women. A total of 30 participants aged 16-20 years and complaining of premenstrual syndrome (PMS) were randomly assigned into two groups: a control group received vitamin B6 and Ca supplements once daily and a study group received the same medical treatment and participated in treadmill training three times per week for 3 months. A premenstrual syndrome questionnaire (MSQ), complete blood picture and hormone assays were performed for the assessment of all participants at the start and after the end of the treatment course. The study group showed a significant decrease in all post-treatment subscale symptoms, scores and total score. Haemoglobin, haematocrit, red cell count and platelet count were significantly increased, while mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and white blood cell count showed no significant differences. There was also a significant decrease in prolactin, oestradiol and progesterone levels. In conclusion, aerobic exercise increases haemoglobin, haematocrit, red cell count and platelet count, and decreases levels of prolactin, oestradiol and progesterone, resulting in improvement of fatigue, impaired concentration, confusion and most premenstrual symptoms. PMID:25279689

  4. Effect of Intensity of Cigarette Smoking on Haematological and Lipid Parameters

    PubMed Central

    Lakshmanan, Anandhi; P, Ganesh Kumar; A, Saravanan

    2014-01-01

    Background: Smoking is the single largest preventable cause of disease and premature death and major tobacco related diseases like cancer costs India more than Rs.308.33 billion per year. Cigarette smoking increases the total peripheral blood leukocyte count but its effect on other haematological parameters is largely unexplored.. Objective: To study the effect of smoking and its intensity on hematological and lipid parameters. Materials and Methods: This cross sectional study included 40 cigarette smokers and 40 non-smokers in the age range of 25 and 40 years at SRM Medical College, Kattangulathur, Tamilnadu, India. In this study the volunteers were otherwise healthy and the smokers were enquired about their intensity of smoking using smoking index. Common hematological and lipid parameters were measured in all subjects. Comparison of smoking status and its intensity among the study subjects were analysed using t-test and ANOVA.. Results: There is a significant increase in levels of haemoglobin, hematocrit, total leukocyte count, total cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL) and reduced levels of high density lipoprotein (HDL) among the smokers. Heavy smokers showed significant dyslipidemia,increase in red blood cell count, total leukocyte count and neutrophil count. Conclusion: Increase in hemoglobin, hematocrit, total leucocyte count and dyslipidemia were found significant among smokers and in heavy smokers there is dyslipidemia with increased RBC count, total leukocyte count with specific increase in neutrophils. As per the existing literature, these changes may lead to future fatal cardiac diseases among the smokers. PMID:25177557

  5. Cytogenetic, immunological, and haematological effects in workers in an ethylene oxide manufacturing plant.

    PubMed

    Van Sittert, N J; de Jong, G; Clare, M G; Davies, R; Dean, B J; Wren, L J; Wright, A S

    1985-01-01

    Samples of blood were collected from a group of plant workers engaged in the manufacture of ethylene oxide (EO) for periods of up to 14 years and also from a group of control personnel matched by age and smoking habits. Peripheral blood lymphocytes were cultured for cytogenetic analysis. Selected immune and haematological parameters were also investigated. The results of these studies showed no statistically significant difference between the group of plant workers and the control group in respect of any of the biological parameters investigated in this study. Nevertheless, duration of employment in EO manufacturing was positively correlated (p less than 0.05) with the frequency of chromosome breaks and with the percentage of neutrophils in a differential white blood cell count and negatively correlated (p less than 0.05) with the percentage of lymphocytes. As the values of these parameters remained within the normal limits of control populations, the correlations were considered to have no significance for health. Atmospheric concentrations of EO were determined using personnel air samplers and were generally below the detection limit (less than 0.05 ppm) during stable plant operations, although transient concentrations of up to 8 ppm were occasionally recorded. The amount of alkylation (2-hydroxyethyl groups) of the Nt atom of histidinyl residues in haemoglobin was also measured in an attempt to gauge recent individual exposures to EO. Variable but, in most instances, readily measurable amounts of Nt-(2'-hydroxyethyl)-L-histidine (Nt represents the N3 atom of histidine) were found in the haemoglobin of plant workers and in the control group who had not knowingly been exposed to an exogenous source of EO. There was no statistically significant difference between the results obtained in the control group and in the group of plant workers. PMID:3965011

  6. A case/control study of adult haematological malignancies in relation to overhead powerlines.

    PubMed Central

    Youngson, J. H.; Clayden, A. D.; Myers, A.; Cartwright, R. A.

    1991-01-01

    A population based case control study of adult haematological malignancy and distance from, and magnetic fields associated with, overhead (OH) power lines has been carried out in the North West and Yorkshire regions of England. Three-thousand, one hundred and forty-four cases with histologically proven disease were entered into the study. One control per case, matched for age, sex, year of diagnosis and health district of residence, was selected from hospital discharges. Seven per cent of cases and controls lived near to OH power lines as defined by the study protocol. The measure of exposure used was the calculated magnetic field strength at each of these addresses due to maximum load currents carried by OH power lines in the 5 years preceding diagnosis. The odds ratio (OR) for living within 50 m of an OH line was 1.29 with a 95% Confidence Interval (CI) of 0.99-1.68 but a chi 2 test for trend with distance was not statistically significant. The analysis of calculated magnetic fields, did not produce any statistically odds ratios. The OR for magnetic fields greater than or equal to 0.1 mG was 1.03 (95% CI 0.81 1.32). Analysis of magnetic fields greater than or equal to 3.0 mG gave an OR of 1.87 (95% CI 0.79 4.42), but this result is based on small numbers. No evidence was found for confounding by the type of dwelling which was used as a partial surrogate for socio-economic status. PMID:2069855

  7. Effect of recombinant human interleukin-3 on haematological recovery from chemotherapy-induced myelosuppression.

    PubMed

    Tepler, I; Elias, A; Kalish, L; Shulman, L; Strauss, G; Skarin, A; Lynch, T; Levitt, D; Resta, D; Demetri, G

    1994-08-01

    Patients with non-small-cell lung cancer (NSCLC) were treated with ICE chemotherapy (ifosfamide 2000 mg/m2, days 1-3; carboplatin 300 mg/m2, day 1; etoposide 75 mg/m2, days 1-3) intravenously (i.v.) during the first 3 d of a maximum of four 28 d treatment cycles. Interleukin-3 (IL-3) was administered in cycles 2 and 4 as a daily subcutaneous (s.c.) injection on days 5-18. Cohorts of three patients were treated at dosage levels of 0.5, 1.25, 2.5, 5.0, 10.0 and 15.0 micrograms/kg/d. At 15.0 micrograms/kg/d a 'flu-like' syndrome of myalgias, arthralgias and fatigue was considered dose-limiting. Other toxicities were headache, fever, urticaria, arrhythmia, chills and flushing. Subsequently, nine patients were added to the group receiving 10 micrograms/kg/d. 27 patients received IL-3 after their second course of ICE. At 10 and 15 micrograms/kg/d, IL-3 in cycle 2 was associated with enhanced haematological recovery. Depth of neutrophil nadir and days of neutropenia (ANC < 0.5 x 10(9)/l) were reduced in 9/13 patients and in 8/11 patients, respectively. No effect was seen on platelet nadir or days of thrombocytopenia. IL-3 was well tolerated up to 10 micrograms/kg/d when given as a daily s.c. injection. Results suggest IL-3 as a potential adjunct to chemotherapy, and further studies to explore administration of IL-3 in combination with other cytokines in this setting are warranted. PMID:7986706

  8. Pre-adaptation, adaptation and de-adaptation to high altitude in humans: cardio-ventilatory and haematological changes.

    PubMed

    Savourey, G; Garcia, N; Besnard, Y; Guinet, A; Hanniquet, A M; Bittel, J

    1996-01-01

    The aim of this study was first to investigate cardio-ventilatory and haematological responses induced by intermittent acclimation and second to study de-adaptation from high altitude observed after descent. To achieve these objectives nine subjects were submitted to intermittent acclimation in a low barometric chamber (8 h daily for 5 days, day 1 at 4500 m, day 5 at 8500 m) before an expedition to the Himalayas. Cardio-ventilatory changes were measured during a hypobaric poikilocapnic hypoxic test (4500 m, barometric pressure = 589 hPa) and haematological changes were studied at sea level. These measurements were performed before and after acclimation, after return to sea level, but also 1 and 2 months after the expedition. In addition, partial pressures of oxygen and carbon dioxide in arterial blood (PaO2, PaCO2) and arterial erythropoietin concentration [EPO] were measured at rest during the hypoxic test. Results suggested the pre-adaptation protocol was efficient since an increased PaO2 (+12%, P < 0.05), a smaller difference in alveolo-arterial PO2 ( -63%, P < 0.05) and a lower PaCO2 ( -11%, P < 0.05), subsequent to ventilatory changes, were observed after acclimation with a significant increase in reticulocytes and in sea level [EPO] (+44% and +62% respectively, P < 0.05). De-adaptation was characterized by a loss of these cardio-ventilatory changes 1 month after descent, whereas the haematological changes (increased red blood cells and packed cell volume, P < 0.05) persisted for 1 month before disappearing 2 months after descent. This study would also suggest that acute hypoxia performed after a sojourn at high altitude could induce significantly depressed EPO responses (P < 0.05). PMID:8817123

  9. Clinical chemistry and haematology historical data in control Sprague-Dawley rats from pre-clinical toxicity studies.

    PubMed

    Petterino, Claudio; Argentino-Storino, Alberta

    2006-01-01

    The purpose of this paper is to provide historical data pertaining to clinical chemistry and haematology parameters, obtained from control Sprague-Dawley rats, used in pre-clinical toxicity studies. Mean, standard deviation, minimum and maximum values for haematological and coagulative profiles, haemato-biochemistry and urine analysis data, and the differences per sex and study duration, 4 versus 13 weeks, are presented. The studies were conducted in agreement with the GLP (Good Laboratory Practice) regulations. Statistically significant differences, at the confidence level of 99%, for the red blood cell (RBC) parameters, the white blood cell (WBC) series parameters, plasmatic albumin/globulin (A/G), alanine amino-transferase (ALT), alkaline phosphatase (ALP), creatinine, globulin, glucose, sodium, total protein, tryglycerides, urea and urine volume were observed in males, when 4-week study values were compared with those obtained from 13-week studies. Female rats showed statistically significant variations, at the confidence level of 99% for RBC number and mean corpuscular haemoglobin (MCH), mean red blood cell volume (MCV), WBCs count and lymphocytes percentage, A/G, albumin, ALT, AST, ALP, creatinine, globulin, and sodium, when 4-week study values were compared to 13-week studies. Similar differences were observed comparing the female with male haematological and biochemical data for the two different times of the sample collection. These data could be useful as a reference for evaluation of background pathology in Sprague-Dawley rats, when used in studies performed to evaluate the toxicological profile of a new chemical entity (NCE) in agreement with requirements from international regulatory agencies. PMID:16343876

  10. Algorithm for pre-emptive glycopeptide treatment in patients with haematologic malignancies and an Enterococcus faecium bloodstream infection

    PubMed Central

    2013-01-01

    Introduction Nowadays Enterococcus faecium has become one of the most emerging and challenging nosocomial pathogens. The aim of this study was to determine risk factors in haematology patients who are at risk of an Enterococcus faecium bloodstream infection (BSI) and should be considered for pre-emptive glycopeptide treatment. With these identified risk factors a prediction model can be developed for clinical use. Methods Retrospectively clinical and microbiological data in 33 patients with an E. faecium BSI were compared to 66 control patients during a 5-year period at the haematology ward. Multivariate logistic regression was used to explore the independent risk factors and a prediction model was developed to determine the risk of an E. faecium BSI. Results E. faecium BSIs were found to be associated with high mortality rates. Independent risk factors for E. faecium BSI were colonization with E. faecium 30 days prior to blood culture (OR 5.71; CI 1.7-18.7), combination of neutropenia and abdominal focus (4.37; 1.4-13.4), age?>?58 years (4.01; 1.3-12.5), hospital stay prior to blood culture?>?14 days (3.55; 0.98-12.9) and CRP (C-reactive protein) level >125 mg/L (4.37; 1.1-10.2). Conclusion Using data from this study, risk stratification for the development of an E. faecium BSI in patients with haematological malignancies is possible. Pre-emptive treatment should be considered in those patients who are at high risk. Using a prediction model as designed in this study, antibiotic stewardship in terms of prudent use of glycopeptides can be improved and might be helpful in controlling further spread of VRE (vancomycin resistant enterococci). PMID:24025668

  11. Is admission screening for Pseudomonas aeruginosa useful in haematologic patients? A prospective study with 1310 patients.

    PubMed

    Sidler, J A; Frei, R; Tschudin-Sutter, S; Dangel, M; Battegay, M; Weisser, M; Passweg, J; Widmer, A F

    2015-06-01

    Screening for Pseudomonas aeruginosa is recommended to guide empirical antimicrobial therapy in patients on high-risk units. However, evidence for this approach is scarce. We therefore screened 1310 patients with severe haematologic diseases for P. aeruginosa colonization at admission: 108 (8.2%) were positive, but only nine (0.7%; six with the same clone as in the screening isolate) subsequently developed a P. aeruginosa bloodstream infection (positive predictive value of screening, 8.6%; negative predictive value of screening, 99.5%). Routine screening for P. aeruginosa at admission did not sufficiently predict subsequent bloodstream infections caused by P. aeruginosa. PMID:25680312

  12. Alternative agents versus prophylactic platelet transfusion for preventing bleeding in patients with haematological disorders after chemotherapy or stem cell transplantation

    PubMed Central

    Estcourt, Lise J; Gregg, Richard; Stanworth, Simon; Doree, Carolyn; Trivella, Marialena; Murphy, Michael F; Tinmouth, Alan

    2014-01-01

    This is the protocol for a review and there is no abstract. The objectives are as follows: To determine whether alternative agents (e.g. artificial platelet substitutes, platelet-poor plasma, fibrinogen, rFVIIa, thrombopoietin mimetics) are as effective and safe as the use of platelet transfusions for the prevention of bleeding (prophylactic platelet transfusion) in patients with haematological disorders who are undergoing myelosuppressive chemotherapy or stem cell transplantation. Antifibrinolytics (lysine analogues) will not be included in this review because they have been the focus of another Cochrane review (Wardrop 2013). PMID:25722650

  13. Risk Factors and Scoring System for Predicting Bacterial Resistance to Cefepime as Used Empirically in Haematology Wards

    PubMed Central

    El Maaroufi, Hicham; Goubard, Agathe; Redjoul, Rabah; Legrand, Patrick; Pautas, Cécile; Mikdame, Mohamed; Doghmi, Kamal; Toma, Andréa; Maury, Sébastien; Schwarzinger, Michael; Cordonnier, Catherine

    2015-01-01

    Objectives. Bacterial resistance is of growing concern in haematology wards. As the inappropriate administration of empirical antibacterial may alter survival, we studied risk factors for resistance to our usual empirical first-line antibacterial therapy, cefepime. Methods. We retrospectively studied 103 first episodes of bacteraemia recorded in our haematology department over 2.5 years. Risk factors for cefepime-resistance were identified by multivariate logistic regression with backward selection (P < 0.05). A scoring system for predicting cefepime-resistance was built on independent factor, with an internal validation by the bootstrap resampling technique. Results. 38 (37%) episodes were due to Gram-negative bacteria. Fifty (49%) were due to bacteria resistant to cefepime. Cefepime resistance was significantly associated with a decreased survival at day 30 (P < 0.05). Three risk factors were independently associated with cefepime-resistance: acute lymphoblastic leukaemia; ?18 days since hospital admission; and receipt of any ?-lactam in the last month. Patients with ?2 of these risk factors had a probability of 86% (CI 95%, 25 to 100%) to carry a cefepime-resistant strain. Conclusion. Using our scoring system should reduce the indication of very broad antibacterial regimens in the empirical, first-line treatment of febrile hematology patients in more than 80% of the cases.

  14. Consensus guidelines for the treatment of yeast infections in the haematology, oncology and intensive care setting, 2014.

    PubMed

    Chen, S C; Sorrell, T C; Chang, C C; Paige, E K; Bryant, P A; Slavin, M A

    2014-12-01

    Pathogenic yeast forms are commonly associated with invasive fungal disease in the immunocompromised host, including patients with haematological malignancies and patients of haemopoietic stem cell transplants. Yeasts include the Candida spp., Cryptococcus spp., Pneumocystis jirovecii and some lesser-known pathogens. Candida species remain the most common cause of invasive yeast infections (and the most common human pathogenic fungi). These guidelines present evidence-based recommendations for the antifungal management of established, invasive yeast infections in adult and paediatric patients in the haematology/oncology setting. Consideration is also given to the critically ill patient in intensive care units, including the neonatal intensive care unit. Evidence for 'pre-emptive' or 'diagnostic-driven antifungal therapy' is also discussed. For the purposes of this paper, invasive yeast diseases are categorised under the headings of invasive candidiasis, cryptococcosis and uncommon yeast infections. Specific recommendations for the management of Pneumocystis jirovecii are presented in an accompanying article (see consensus guidelines by Cooley et?al. appearing elsewhere in this supplement). PMID:25482743

  15. Towards optimal clinical and epidemiological registration of haematological malignancies: Guidelines for recording progressions, transformations and multiple diagnoses.

    PubMed

    Gavin, Anna; Rous, Brian; Marcos-Gragera, Rafael; Middleton, Richard; Steliarova-Foucher, Eva; Maynadie, Marc; Zanetti, Roberto; Visser, Otto

    2015-06-01

    Haematological malignancies (HM) represent over 6% of the total cancer incidence in Europe and affect all ages, ranging between 45% of all cancers in children and 7% in the elderly. Thirty per cent of childhood cancer deaths are due to HM, 8% in the elderly. Their registration presents specific challenges, mainly because HM may transform or progress in the course of the disease into other types of HM. In the context of cancer registration decisions have to be made about classifying subsequent notifications on the same patient as the same tumour (progression), a transformation or a new tumour registration. Allocation of incidence date and method of diagnosis must also be standardised. We developed European Network of Cancer Registries (ENCR) recommendations providing specific advice for cancer registries to use haematology and molecular laboratories as data sources, conserve the original date of incidence in case of change of diagnosis, make provision for recording both the original as well as transformed tumour and to apply precise rules for recording and counting multiple diagnoses. A reference table advising on codes which reflect a potential transformation or a new tumour is included. This work will help to improve comparability of data produced by population-based cancer registries, which are indispensable for aetiological research, health care planning and clinical research, an increasing important area with the application of targeted therapies. PMID:24630945

  16. Contribution of new diagnostic approaches to antifungal treatment plans in high-risk haematology patients.

    PubMed

    Einsele, H; Loeffler, J

    2008-05-01

    In high-risk patient cohorts, such as patients after solid-organ or allogeneic stem-cell transplantation, or patients with acute leukaemia, early diagnosis of invasive fungal infections (IFIs) is essential, as delayed or missing diagnosis of IFI results in increasing rates of mortality. However, diagnosis of most IFIs, especially of invasive aspergillosis, is difficult because classic tests have low sensitivity and specificity, and radiology often provides non-specific and transient results. The limited sensitivity and specificity of conventional assays for the detection of IFI and the growing number of immunocompromised patients who are at risk for opportunistic fungal infections have led to the development of new assays. These methods include antigen detection systems, such as ELISAs, and different molecular methods (PCR assays). Serological tests, such as the detection of the carbohydrate galactomannan, are standardised and commercially available. However, they still need to be evaluated in large patient cohorts, especially children. The benefit of antibody detection remains unclear if patients are under immune suppression or are heavily colonised but not infected. A range of different PCR assays (conventional, nested, real-time) have been developed, targeting different gene regions (cytochrome P450, heat-shock proteins, 18S, 5.8S, 28S, internal transcribed spacer), including a variety of amplicon detection methods, such as gel electrophoresis, hybridisation with specific probes, ELISA and restriction fragment length polymorphism. These molecular assays provide high potential in terms of sensitivity and specificity, but vary widely in their feasibility and up to now have not been standardised. Taken together, new non-culture-based diagnostic assays are appropriate as simple and rapid screening tests with high sensitivities and quick turnaround times. Thus, they might help to reduce empirical antifungal therapy and might be valuable tools to allow early initiation and monitoring of pre-emptive antifungal therapy. In this review, we assess the performance of a variety of non-culture-based tests for the detection of IFI in high-risk haematological patients, with emphasis on the impact of the assays on different management strategies. PMID:18430128

  17. The distribution patterns of trace elements in the blood and organs in a rabbit experimental model of copper pollution and study of haematology and biochemistry parameters

    Microsoft Academic Search

    Shi-Gang Shen; Hui Li; Yan-Yan Zhao; Qiu-Yan Zhang; Han-Wen Sun

    2005-01-01

    In a rabbit model of five copper overfeeding, we investigated the distribution pattern of trace elements of copper [Cu], zinc [Zn], chromium [Cr], manganese [Mn], and selenium [Se] in blood serum, red cell, and whole blood and in the organs: brain, gallbladder, liver, intestines, heart, kidney, lung, and spleen. Furthermore, their haematology and biochemistry parameters as well as feed consumption

  18. The effect of copper and zinc at neutral and acidic pH on the general haematology and osmoregulation of Oreochromis mossambicus

    Microsoft Academic Search

    G Nussey; J HJ van Vuren; H H du Preez

    2002-01-01

    The objective of this study was to determine the effect of sublethal copper and zinc concentrations at a neutral and an acidic pH, on selected haematological parameters as well as on the total osmolality and electrolyte concentrations of Oreochromis mossambicus. In general, at neutral pH copper and zinc caused blood acidosis, increases in circulating white blood cell numbers, causing stimulation

  19. Epidemiology and outcome of candidaemia in patients with oncological and haematological malignancies: results from a population-based surveillance in Spain.

    PubMed

    Puig-Asensio, M; Ruiz-Camps, I; Fernández-Ruiz, M; Aguado, J M; Muńoz, P; Valerio, M; Delgado-Iribarren, A; Merino, P; Bereciartua, E; Fortún, J; Cuenca-Estrella, M; Almirante, B

    2015-05-01

    A prospective, population-based surveillance on candidaemia was implemented in five metropolitan areas of Spain from May 2010 to April 2011. We aimed to describe the distribution and susceptibility pattern of Candida species, and to evaluate risk factors for mortality in patients with oncological (solid tumours) and haematological malignancies. Adults (?16 years) with cancer were included in the present report. Impact of therapeutic strategies on 7- and 30-day mortality were analysed by logistic regression, adjusting for propensity score by inverse weighting probability of receiving early antifungal treatment and catheter removal. We included 238 (32.6%) patients (195 oncological, 43 haematological). Compared with oncological patients, haematological patients were more likely to have received chemotherapy (53.5% versus 17.4%, p < 0.001) or corticosteroids (41.9% versus 21%, p < 0.001), and have neutropenia (44.2% versus 1.5%, p < 0.001). Overall, 14.8% of patients developed breakthrough candidaemia. Non-albicans Candida species (71.1% versus 55.6%, p 0.056) and Candida tropicalis (22.2% versus 7.6%, p 0.011) were more frequent in haematological patients. Based on EUCAST breakpoints, 27.6% of Candida isolates were non-susceptible to fluconazole. Resistance to echinocandins was negligible. Mortality at 7 and 30 days was 12.2% and 31.5%, respectively, and did not differ significantly between the patient groups. Prompt antifungal therapy together with catheter removal (?48 hours) was associated with lower mortality at 7 days (adjusted OR 0.05; 95% CI 0.01-0.42) and 30 days (adjusted OR 0.27; 95% CI 0.16-0.46). In conclusion, non-albicans species are emerging as the predominant isolates, particularly in haematological patients. Prompt, adequate antifungal treatment plus catheter removal may lead to a reduction in mortality. PMID:25703212

  20. Blood Haematology, Serum Thyroid Hormones and Glutathione Peroxidase Status in Kacang Goats Fed Inorganic Iodine and Selenium Supplemented Diets

    PubMed Central

    Aghwan, Z. A.; Sazili, A. Q.; Alimon, A. R.; Goh, Y. M.; Hilmi, M.

    2013-01-01

    The effects of dietary supplementation of selenium (Se), iodine (I), and a combination of both on the blood haematology, serum free thyroxine (FT4) and free triiodothyronine (FT3) hormones and glutathione peroxidase enzyme (GSH-Px) activity were examined on twenty four (7 to 8 months old, 22±1.17 kg live weight) Kacang crossbred male goats. Animals were randomly assigned to four dietary treatments (6 animals in each group). Throughout 100 d of feeding trial, the animals of control group (CON) received a basal diet, while the other three groups were offered basal diet supplemented with 0.6 mg/kg diet DM Se (SS), or 0.6 mg/kg diet DM I (PI), or a combination of both Se and I, each at 0.6 mg/kg diet DM (SSPI). The haematological attributes which are haemoglobin (Hb), red blood cell (RBC), packed cell volume (PCV), mean cell volume (MCV), white blood cells (WBC), band neutrophils (B Neut), segmented neutrophils (S Neut), lymphocytes (Lymph), monocytes (Mono), eosinophils (Eosin) and basophils (Baso) were similar among the four treatment groups, while serum levels of Se and I increased significantly (p<0.05) in the supplemented groups. The combined dietary supplementation of Se and I (SSPI) significantly increased serum FT3 in the supplemented animals. Serum GSH-Px activity increased significantly in the animals of SS and SSPI groups. It is concluded that the dietary supplementation of inorganic Se and I at a level of 0.6 mg/kg DM increased serum Se and I concentration, FT3 hormone and GSH-Px activity of Kacang crossbred male goats. PMID:25049744

  1. Growth and haematological response of indigenous Venda chickens aged 8 to 13 weeks to varying dietary lysine to energy ratios.

    PubMed

    Alabi, O J; Ng'ambi, J W; Mbajiorgu, E F; Norris, D; Mabelebele, M

    2015-06-01

    The effect of feeding varying dietary lysine to energy levels on growth and haematological values of indigenous Venda chickens aged 8 - 13 weeks was evaluated. Four hundred and twenty Venda chickens (BW 362 ± 10 g) were allocated to four dietary treatments in a completely randomized design. Each treatment was replicated seven times, and each replicate had fifteen chickens. Four maize-soya beans-based diets were formulated. Each diet had similar CP (150 g/kg DM) and lysine (8 g lysine/kg DM) but varying energy levels (11, 12, 13 and 14 MJ ME/kg DM). The birds were reared in a deep litter house; feed and water were provided ad libitum. Data on growth and haematological values were collected and analysed using one-way analysis of variance. Duncan's test for multiple comparisons was used to test the significant difference between treatment means (p < 0.05). A quadratic equation was used to determine dietary lysine to energy ratios for optimum parameters which were significant difference. Results showed that dietary energy level influenced (p < 0.05) feed intake, feed conversion ratio, live weight, haemoglobin and pack cell volume values of chickens. Dry matter digestibility, metabolizable energy and nitrogen retention not influenced by dietary lysine to energy ratio. Also, white blood cell, red blood cell, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration in female Venda chickens aged 91 days were not influenced by dietary lysine to energy ratio. It is concluded that dietary lysine to energy ratios of 0.672, 0.646, 0.639 and 0.649 optimized feed intake, growth rate, FCR and live weight in indigenous female Venda chickens fed diets containing 8 g of lysine/kg DM, 150 g of CP/kg DM and 11 MJ of ME/kg DM. This has implications in diet formulation for indigenous female Venda chickens. PMID:25495676

  2. Micro-organisms Associated with Febrile Neutropenia in Patients with Haematological Malignancies in a Tertiary Care Hospital in Eastern India.

    PubMed

    Mandal, Prakas Kumar; Maji, Suman Kumar; Dolai, Tuphan Kanti; De, Rajib; Dutta, Shyamali; Saha, Sandeep; Bhattacharyya, Maitreyee

    2015-03-01

    There is paucity of information from eastern India with regard to observed dominant micro-organisms causing febrile neutropenia (FN) in patients with haematological malignancies. To identify the prevalence of pathogenic microorganisms associated with FN. A total number of 268 episodes of FN were analysed from September'2010 to October'2013. The blood samples were inoculated into brain heart infusion broth, glucose broth, Hicombi dual performance media (Himedia, LQ-12) at 37° C for 168 h and Bactec method was also performed for these samples. Blood agar, chocolate agar, MacConkey's agar and cystine lactose electrolyte deficient agar were used for isolation of the microorganisms. A total number of 78 (29.10 %) episodes revealed positive growths. Gram negative bacilli and Gram positive cocci were isolated in 61.53 and 34.61 % cases respectively. The eight commonest isolates were Pseudomonas aeruginosa (14.10 %), methicillin resistant Staphylococcus aureus (MRSA-12.82 %), Acinetobacter sps (11.53 %), coagulase negative Staphylococcus (10.25 %), Klebsiella pneumoniae (8.97 %), Escherichia coli (8.97 %), ESBL E. coli (6.41 %), methicillin sensitive S. aureus (MSSA-6.41 %). Amongst other less common isolates were Citrobacter kosseri (3.84 %), Citrobacter freundii (2.56 %), Ralstonia paucula (2.56 %), Cedecia neteri (1.28 %), methicillin resistant coagulase negative Staphylococcus (2.56 %). Candida spp. including two cases of Candida non-albicans was isolated in 3.84 % of cases. P. aeruginosa was the commonest pathogenic isolates in FN patients associated with haematological malignancies in this study. Gram negative bacteria were the commonest isolates in FN including significant numbers of rare opportunistic micro-organisms. PMID:25548444

  3. Ethanol versus heparin locks for the prevention of central venous catheter-associated bloodstream infections: a randomized trial in adult haematology patients with Hickman devices.

    PubMed

    Worth, L J; Slavin, M A; Heath, S; Szer, J; Grigg, A P

    2014-09-01

    The effectiveness of ethanol locks for prevention of central venous catheter (CVC)-associated bloodstream infection (CLABSI) in adult haematology patients has not been thoroughly evaluated. This study aimed to compare prospectively heparinized saline with 70% ethanol locks using 2 h dwell time in patients with tunnelled CVCs. In saline (N = 43) and ethanol (N = 42) groups, CLABSI rates were 6.0 [95% confidence interval (CI): 3.4-9.8] and 4.1 (95% CI: 1.9-7.7) per 1000 CVC days, respectively (P = 0.42). In the ethanol group, two exit-site infections and one tunnel/pocket infection were observed. Reduction in device-associated infection was not achieved with prophylactic 70% ethanol locks in patients with haematological malignancy and tunnelled CVCs. PMID:25063013

  4. Fixed-dose single administration of Pegfilgrastim vs daily Filgrastim in patients with haematological malignancies undergoing autologous peripheral blood stem cell transplantation

    Microsoft Academic Search

    P B Staber; R Holub; W Linkesch; H Schmidt; P Neumeister

    2005-01-01

    Infectious complications are frequent events in patients undergoing high-dose cytotoxic chemotherapy with subsequent autologous peripheral blood stem cell transplantation (PBSCT). To evaluate whether a single subcutaneous injection of pegfilgrastim (6 mg) is as safe and effective as daily filgrastim (5 ?g\\/kg\\/day), 60 consecutive autologous stem cell transplantations performed for various haematological malignancies have been analysed. In total, 24 patients undergoing

  5. Influence of esterified-glucomannan on performance and organ morphology, serum biochemistry and haematology in broilers exposed to individual and combined mycotoxicosis (aflatoxin, ochratoxin and T-2 toxin)

    Microsoft Academic Search

    M. V. L. N. Raju; G. Devegowda

    2000-01-01

    1. A study was conducted to evaluate the individual and combined effects of aflatoxin B 1 (AF), ochratoxin A (OA) and T-2 toxin (T-2) on performance, organ morphology, serum biochemistry and haematology of broiler chickens and the efficacy of esterified-glucomannan (E-GM), a cell wall derivative of Saccharomyces cerevisiae 1026 in their counteraction. 2. Two dietary inclusion rates of AF (0

  6. Selected Haematological and Biochemical Parameters of Blood in Rats After Subchronic Administration of Vanadium and\\/or Magnesium in Drinking Water

    Microsoft Academic Search

    Agnieszka ?cibior; Halina Zaporowska; Jaros?aw Ostrowski

    2006-01-01

    The purpose of these studies was to evaluate the effect of selected vanadium and magnesium doses on certain haematological\\u000a and biochemical blood parameters in rats. Outbred 2-month-old, albino male Wistar rats received for a period of 6 weeks, as\\u000a a sole drinking liquid, the following water solutions: group II, sodium metavanadate (SMV) at a concentration of 0.125 mg\\u000a V\\/mL; group

  7. Protective role of propolis in chlorpyrifos-induced changes in the haematological parameters and the oxidative/antioxidative status of Cyprinus carpio carpio.

    PubMed

    Enis Yonar, M; Yonar, Serpil Mi?e; Ural, Mevlüt ?ener; Silici, Sibel; Dü?ükcan, Mustafa

    2012-08-01

    The protective effect of propolis on haematological parameters and antioxidant status were evaluated in the blood and various tissues of carp exposed to chlorpyrifos (CPF). The fish were exposed to sublethal concentrations of CPF (0.040 and 0.080 mg/L) for 10 days, and propolis (10 mg per kg of fish weight) was simultaneously administered. Samples of the blood and tissue (liver, kidney, and gill) were collected at the end of the experiment and analysed for their oxidant-antioxidant status, including the malondialdehyde (MDA) levels, and the superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activity. The samples were also measured for changes in the haematological parameters, such as the red blood cell (RBC) and white blood cell (WBC) counts, the haemoglobin concentration (Hb), the haematocrit (Ht) level, and the erythrocyte indices: the mean corpuscular volume (MCV), the mean corpuscular haemoglobin (MCH) and the mean corpuscular haemoglobin concentration (MCHC). The findings of this study demonstrated that CPF had a negative effect on the haematological parameters and the antioxidant enzyme activities of the fish; this toxic effect was neutralised by the administration of propolis. The present results suggest that propolis can be effective in the protection of CPF-induced toxicity in fish. PMID:22634289

  8. Effect of a Probiotic Containing Bacillus licheniformis and Bacillus subtilis and Ferroin Solution on Growth Performance, Body Composition and Haematological Parameters in Kutum (Rutilus frisii kutum) Fry.

    PubMed

    Azarin, Hajar; Aramli, Mohammad Sadegh; Imanpour, Mohammad Reza; Rajabpour, Mina

    2015-03-01

    This study aimed to assess the efficacy of BioPlus 2B, a probiotic containing Bacillus licheniformis and B. subtilis and Ferroin solution on growth performance, body composition and haematological parameters in kutum, Rutilus frisii kutum, fry. The fish were fed dry pellets containing various ratios of probiotics and Ferroin for 60 days after absorption of the yolk sac. At the end of the trial, growth indices (final weight, weight gain, specific growth rate, daily growth rate, food conversion ratio and condition factor), body composition (crude protein, crude lipid, ash and moisture) and haematological parameters [haematocrit (Hct), haemoglobin (Hb), red blood cells (RBC), white blood cells (WBC), neutrophils (NEUTR), lymphocytes (LYM), mean cell volume (MCV), mean cell haemoglobin (MCH) and mean cell haemoglobin concentration (MCHC)] were assessed. Regarding body composition, total protein levels were higher, and ash, moisture and lipid levels were lower in fish receiving the probiotic and Ferroin treatments compared with the control group (P < 0.05). Fish receiving diets supplemented with probiotics and Ferroin solution showed significantly better growth than those fed the basal diet (control). RBC, Hct, Hb, MCV, MCH and LYM were all highest in fish fed probiotic (1.6 × 10(9) CFU/g dry pellet) + Ferroin solution (7 mg/kg dry pellet) + dry pellets. These results indicate that the combination of probiotic and Ferroin solution represents an effective dietary supplement for improving carcass quality, growth performance and haematological parameters in kutum fry. PMID:25431124

  9. Molecular epidemiology of catheter-related bloodstream infections caused by coagulase-negative staphylococci in haematological patients with neutropenia.

    PubMed Central

    Müller-Premru, M.; Cernelc, P.

    2004-01-01

    Catheter-related bloodstream infection (CRBSI) caused by coagulase-negative staphylococci (CNS) is common in haematological patients with febrile neutropenia. As the clinical signs of CRBSI are usually scarce and it is difficult to differentiate from blood culture contamination, we tried to confirm CRBSI by molecular typing of CNS isolated from paired blood cultures (one from a peripheral vein and another from the central venous catheter hub). Blood cultures were positive in 59 (36%) out of 163 patients. CNS were isolated in 24 (40%) patients; in 14 from paired blood cultures (28 isolates) and in 10 from a single blood culture. CNS from paired blood cultures were identified as Staphylococcus epidermidis. Antimicrobial susceptibility was determined and bacteria were typed by pulsed-field gel electrophoresis (PFGE) of bacterial genomic DNA. In 13 patients, the antibiotic susceptibility of isolates was identical. The PFGE patterns from paired blood cultures were identical or closely related in 10 patients, thus confirming the presence of CRBSI. In the remaining four patients they were unrelated, and suggested a mixed infection or contamination. Since CNS isolates from three patients had identical PFGE patterns, they were probably nosocomially spread amongst them. PMID:15473156

  10. Epidemiology and sites of involvement of invasive fungal infections in patients with haematological malignancies: a 20-year autopsy study.

    PubMed

    Lewis, Russell E; Cahyame-Zuniga, Lizebeth; Leventakos, Konstantinos; Chamilos, Georgios; Ben-Ami, Ronen; Tamboli, Pheroze; Tarrand, Jeffrey; Bodey, Gerald P; Luna, Mario; Kontoyiannis, Dimitrios P

    2013-11-01

    Autopsy studies remain an essential tool for understanding the patterns of fungal disease not detected ante mortem with current diagnostic approaches. We collected data concerning the microbiological trends, patient clinical characteristics and sites of involvement for invasive fungal infections (IFIs) identified at autopsy in a single large cancer treatment centre over a 20-year period (1989-2008). The autopsy rate and IFI prevalence both declined significantly during the study period. The prevalence of Aspergillus spp. decreased significantly from the first 15 years of the study (from 0.12 to 0.14 cases per 100 autopsies to 0.07 in 2004-2008; P = 0.04), with only Mucorales accounting for a greater proportion of IFIs over the duration of the study period (0.06 to 0.2 cases per 100 autopsies, P = 0.04). After 2003, moulds accounted for the majority of infections identified at autopsy in the spleen, kidney, heart and gastrointestinal tract. Despite a trend of decreasing prevalence from 1989 to 2004, invasive candidiasis increased in prevalence during later periods 2004-2008 (0.02-0.05 per 100 autopsies) with decreasing kidney, heart and spleen involvement. Despite a declining autopsy rate, these data suggest a decreasing prevalence overall of IFIs with changing patterns of dissemination in patients with haematological malignancies. PMID:23551865

  11. The Effect of Mycotoxin Deoxynivalenol on Haematological and Biochemical Indicators and Histopathological Changes in Rainbow Trout (Oncorhynchus mykiss)

    PubMed Central

    Modra, Helena; Blahova, Jana; Franc, Ales; Fictum, Petr; Sevcikova, Marie; Svobodova, Zdenka

    2014-01-01

    Deoxynivalenol (DON), produced by the Fusarium genus, is a major contaminant of cereal grains used in the production of fish feed. The effect of mycotoxin deoxynivalenol on rainbow trout (Oncorhynchus mykiss) was studied using a commercial feed with the addition of DON in a dose of 2?mg/kg feed. The fish (n = 40) were exposed to the mycotoxin for 23 days. The trout were divided into two groups, control and experimental groups. Control groups were fed a commercial feed naturally contaminated with a low concentration of DON (225??g/kg feed); experimental groups were fed a commercial feed with the addition of DON (1964??g/kg feed). Plasma biochemical and haematological indices, biometric parameters, and histopathological changes were assessed at the end of the experiment. The experimental groups showed significantly lower values in MCH (P < 0.05). In biochemical indices, after 23-day exposure, a significant decrease in glucose, cholesterol (P < 0.05), and ammonia (P < 0.01) was recorded in the experimental group compared to the control group. Our assessment showed no significant changes in biometric parameters. The histopathological examination revealed disorders in the caudal kidney of the exposed fish. The obtained data show the sensitivity of rainbow trout (O. mykiss) to deoxynivalenol. PMID:24729967

  12. Dietary supplementation with chitin and chitosan on haematology and innate immune response in Epinephelus bruneus against Philasterides dicentrarchi.

    PubMed

    Harikrishnan, Ramasamy; Kim, Ju-Sang; Balasundaram, Chellam; Heo, Moon-Soo

    2012-05-01

    The present study investigated the effect of 1.0% chitin and chitosan supplementation diets on haematology and immune response in Kelp grouper, Epinephelus bruneus against protozoan parasite, Philasterides dicentrarchi. The red blood cells (RBC), white blood cells (WBC), haemoglobin levels, lymphocytes, monocytes, and neutrophils significantly increased in kelp grouper fed with chitin or chitosan enriched diets against P. dicentrarchi. The mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), and thrombocytes did not significantly change against pathogen. The phagocytic activity, respiratory burst activity, complement activity, antiprotease activity, and ?2-macroglobulin were significantly enhanced in fish fed with 1% chitin and chitosan diet on weeks 2 and 4. The lysozyme activity, total protein, and myeloperoxidase activity significantly increased in fish fed with chitin or chitosan supplementation diet from weeks 1 to 4 against pathogen. The cumulative mortality was found low in fish fed with chitin and chitosan enriched diets than those of control against pathogen. The present study suggests that supplementation of 1.0% chitin or chitosan in diets positively enhances immune response and affords disease resistance in kelp grouper, E. bruneus against P. dicentrarchi infection. PMID:22475776

  13. The effect of mycotoxin deoxynivalenol on haematological and biochemical indicators and histopathological changes in rainbow trout (Oncorhynchus mykiss).

    PubMed

    Matejova, Iveta; Modra, Helena; Blahova, Jana; Franc, Ales; Fictum, Petr; Sevcikova, Marie; Svobodova, Zdenka

    2014-01-01

    Deoxynivalenol (DON), produced by the Fusarium genus, is a major contaminant of cereal grains used in the production of fish feed. The effect of mycotoxin deoxynivalenol on rainbow trout (Oncorhynchus mykiss) was studied using a commercial feed with the addition of DON in a dose of 2 mg/kg feed. The fish (n=40) were exposed to the mycotoxin for 23 days. The trout were divided into two groups, control and experimental groups. Control groups were fed a commercial feed naturally contaminated with a low concentration of DON (225? ?g/kg feed); experimental groups were fed a commercial feed with the addition of DON (1964? ?g/kg feed). Plasma biochemical and haematological indices, biometric parameters, and histopathological changes were assessed at the end of the experiment. The experimental groups showed significantly lower values in MCH (P<0.05). In biochemical indices, after 23-day exposure, a significant decrease in glucose, cholesterol (P<0.05), and ammonia (P<0.01) was recorded in the experimental group compared to the control group. Our assessment showed no significant changes in biometric parameters. The histopathological examination revealed disorders in the caudal kidney of the exposed fish. The obtained data show the sensitivity of rainbow trout (O. mykiss) to deoxynivalenol. PMID:24729967

  14. Toxicity of anthraquinones: differential effects of rumex seed extracts on rat organ weights and biochemical and haematological parameters.

    PubMed

    Islam, Rabigul; Mamat, Yultuz; Ismayil, Ilyar; Yan, Ming; Kadir, Mahsutjan; Abdugheny, Abdujilil; Rapkat, Haximjan; Niyaz, Mardan; Ali, Yusupjan; Abay, Sirapil

    2015-05-01

    The genus Rumex and related species such as Rheum and Polygonum are widely used as medicinal herbs and foods. They contain anthraquinones (AQ) such as emodin and chrysophanol as active ingredients, and there is concern about the toxicity of these compounds. This study evaluated the chronic effects of Rumex patientia seed aqueous and ethanolic extracts, in male and female rats separately, on organ weights and over 30 haematological, biochemical and histological parameters, immediately after 14-week administration and after a further period of 15?days without drug treatment. Adverse changes were associated with long-term AQ administration, and these focussed on the liver, lung and kidney, but after 15-day convalescence, most had reverted to normal. In general, male rats appeared to be more susceptible than female rats at similar doses. The water extract produced no irreversible changes, which may reflect the lower dose of the AQ constituents or the presence of different ancillary compounds, and supports the traditional method of extracting Rumex seeds with water. In conclusion, ethanolic extracts of R. patientia caused irreversible pathological changes at very high doses (4000mg/kg), but lower doses and aqueous extracts produced either non-significant or reversible changes. Long-term administration of high doses of AQ extracts over a long period of time should be avoided until further assurances can be given, and given other existing reports of reproductive toxicity, should be avoided altogether during pregnancy. Copyright © 2015 John Wiley & Sons, Ltd. PMID:25753342

  15. Haematopoietic stem cell transplantation for Diamond Blackfan anaemia: a report from the Italian Association of Paediatric Haematology and Oncology Registry.

    PubMed

    Fagioli, Franca; Quarello, Paola; Zecca, Marco; Lanino, Edoardo; Corti, Paola; Favre, Claudio; Ripaldi, Mimmo; Ramenghi, Ugo; Locatelli, Franco; Prete, Arcangelo

    2014-06-01

    Allogeneic haematopoietic stem cell transplantation (HSCT) is the only curative option for patients with Diamond Blackfan anaemia (DBA). We report the transplantation outcome of 30 Italian DBA patients referred to the Italian Association of Paediatric Haematology and Oncology Registry between 1990 and 2012. This is one of the largest national registry cohorts of transplanted DBA patients. Most patients (83%) were allografted after 2000. A matched sibling donor was employed in 16 patients (53%), the remaining 14 patients (47%) were transplanted from matched unrelated donors. Twenty-eight of the 30 patients engrafted. One patient died at day +6 due to veno-occlusive disease without achieving neutrophil recovery and another patient remained transfusion-dependent despite the presence of a full donor chimerism. The 5-year overall survival and transplant-related mortality was 74·4% and 25·6%, respectively. Patients younger than 10 years as well as those transplanted after 2000 showed a significantly higher overall survival and a significantly lower risk of transplant-related mortality. No difference between donor type was observed. Our data suggest that allogeneic HSCT from a related or unrelated donor was a reasonable alternative to transfusion therapy in young and well chelated DBA patients. PMID:24611452

  16. Consensus guidelines for the use of empiric and diagnostic-driven antifungal treatment strategies in haematological malignancy, 2014.

    PubMed

    Morrissey, C O; Gilroy, N M; Macesic, N; Walker, P; Ananda-Rajah, M; May, M; Heath, C H; Grigg, A; Bardy, P G; Kwan, J; Kirsa, S W; Slavin, M; Gottlieb, T; Chen, S

    2014-12-01

    Invasive fungal disease (IFD) causes significant morbidity and mortality in patients undergoing allogeneic haemopoietic stem cell transplantation or chemotherapy for haematological malignancy. Much of these adverse outcomes are due to the limited ability of traditional diagnostic tests (i.e. culture and histology) to make an early and accurate diagnosis. As persistent or recurrent fevers of unknown origin (PFUO) in neutropenic patients despite broad-spectrum antibiotics have been associated with the development of IFD, most centres have traditionally administered empiric antifungal therapy (EAFT) to patients with PFUO. However, use of an EAFT strategy has not been shown to have an overall survival benefit and is associated with excessive antifungal therapy use. As a result, the focus has shifted to developing more sensitive and specific diagnostic tests for early and more targeted antifungal treatment. These tests, including the galactomannan enzyme-linked immunosorbent assay and Aspergillus polymerase chain reaction (PCR), have enabled the development of diagnostic-driven antifungal treatment (DDAT) strategies, which have been shown to be safe and feasible, reducing antifungal usage. In addition, the development of effective antifungal prophylactic strategies has changed the landscape in terms of the incidence and types of IFD that clinicians have encountered. In this review, we examine the current role of EAFT and provide up-to-date data on the newer diagnostic tests and algorithms available for use in EAFT and DDAT strategies, within the context of patient risk and type of antifungal prophylaxis used. PMID:25482742

  17. Real-world costs of autologous and allogeneic stem cell transplantations for haematological diseases: a multicentre study.

    PubMed

    Blommestein, H M; Verelst, S G R; Huijgens, P C; Blijlevens, N M A; Cornelissen, J J; Uyl-de Groot, C A

    2012-12-01

    Haematopoietic stem cell transplantation (SCT) is an expensive lifesaving procedure, which is increasingly performed in patients with haematological diseases. Developments in the protocol for SCT have resulted in cost estimates that require updating. We aimed to calculate actual costs for SCT and to identify major cost drivers by means of a daily practice cost study. We randomly selected 191 patients, treated at three university hospitals, who underwent an autologous (auto) SCT or allogeneic (allo) SCT in 2007, 2008 or 2009. Allo-SCT included sibling donors, matched unrelated donors (MUD) and umbilical cord blood (UCB). Resource use was collected from the hospital registration systems and medical files. The total costs included selection and harvesting of stem cells, transplantation and 1-year follow-up. The average costs per patient were 45,670 € for auto-SCT and 101,919 € for sibling allo-SCT. The costs of transplantations from unrelated donors were much higher: 171,478 € for allo-SCT-MUD and 254,689 € for allo-SCT-UCB. Hospital inpatient days together with laboratory and other activities were the main cost drivers across all types of SCT. Besides, donor search costs were a large cost component in allo-SCT-sib (18 %) and allo-SCT-MUD (12 %). Real-world costs were above routine reimbursement and appropriate financing is necessary to guarantee the continuation of SCT. The costs calculated in this study provide reliable up-to-date input for cost-effectiveness studies and budget revision. PMID:22864761

  18. Immunophenotypic analysis of cerebrospinal fluid cell populations with the Cell-Dyn Sapphire haematology analyser: method feasibility and preliminary observations.

    PubMed

    Adam, P; Sobek, O; Scott, C S; Dolezil, D; Kasik, J; Hajdukova, L; Adam, D

    2010-02-01

    Cerebrospinal fluid (CSF) samples (n=50) from patients with neurological disease (bacterial infection, viral infection, neuroborreliosis and multiple sclerosis) were analysed to characterize cell populations by fluorescent immunocytometry with the CD-Sapphire haematology analyser. Reagent combinations applied to all CSF samples comprised CD3/CD19/HLA-DR and CD4/CD8, with some being further analysed using CD3/CD4, CD3/CD16 and CD3/CD25 protocols. Of the 50 samples, 11 were excluded because of high proportions of nonviable cells (n=2) or insufficient cell numbers (n=9). Apart from bacterial infection with granulocytosis, all diagnostic groups showed high proportions (51.4-77.0%) of CD3+ T cells. There was a modest association between T-cell and B-cell counts, but absolute B-cell numbers exceeded 5 cells/microl in only 7/39 cases (neuroborreliosis, n=6; bacterial meningitis, n=1). CD3/Ia antigen (activation) co-expression was low and only exceeded 5% in 7/39 samples with no diagnostic correlation. Primary CD4+ and CD8+ T-cell subsets showed similar quantitative trends and CD4/CD8 co-analysis revealed the presence in all diagnostic groups (neuroborreliosis and multiple sclerosis in particular) of a CD4+CD8int fraction that was predominantly CD3+ and CD16- and had a morphological profile consistent with small lymphoid cells. Supplementary CD-Sapphire cellular immunological analysis of most CSF samples is feasible using the procedure detailed in this communication. PMID:19500178

  19. Diagnostic and therapeutic approach to persistent or recurrent fevers of unknown origin in adult stem cell transplantation and haematological malignancy.

    PubMed

    Morrissey, C O; Bardy, P G; Slavin, M A; Ananda-Rajah, M R; Chen, S C; Kirsa, S W; Ritchie, D S; Upton, A

    2008-06-01

    Persistent or recurrent fevers of unknown origin (PFUO) in neutropenic patients on broad-spectrum antibiotics have traditionally been treated with empirical antifungal therapy (EAFT). The lack of survival benefit seen with the use of amphotericin B deoxycholate (AmB-D) as EAFT has been attributed to its toxicities. More recently, newer, less toxic and more expensive antifungal agents such as the lipid formulations of AmB, the newer azoles (fluconazole, itraconazole and voriconazole) and caspofungin have been analysed in a number of EAFT trials. Compared with AmB-D the newer agents have superior safety but are of equivalent efficacy. This lack of survival advantage is related to the fact that the trigger for commencement of EAFT is late and non-specific. Thus, alternative approaches are required. New sensitive serological and molecular tests for the detection of Aspergillus antigens and genomic DNA have been developed and evaluated in accuracy studies. These tests have been incorporated into management strategies (i.e. pre-emptive strategies) to direct antifungal therapy. The pre-emptive approach has been shown to be safe and feasible but its impact on clinically important patient outcomes such as survival is less clear. Other advances include the introduction of effective, non-toxic mould-active antifungal prophylaxis and patient risk-group stratification. In this paper we provide new evidence-based algorithms for the diagnosis and treatment of PFUO in adult patients undergoing stem cell transplantation and chemotherapy for haematological malignancy which incorporate these newer diagnostic tests and are directed by the risk category of the patient and type of antifungal prophylaxis the patient is receiving. PMID:18588521

  20. Haematological and biochemical changes in horses competing in a 3 Star horse trial and 3-day-event.

    PubMed

    Andrews, F M; Geiser, D R; White, S L; Williamson, L H; Maykuth, P L; Green, E M

    1995-11-01

    Haematological and biochemical changes in horses competing in the Endurance Test (Phase T and D) of an advanced Horse Trial (HT, n = 22) and the Endurance Test (Phases A-D) of an advanced (CCI) 3-day-event (TD, n = 11) over a similar course on the same day were studied. Environmental conditions during the event were cool (5.5-11.1 degrees C). Blood samples were collected from the horses in each group the evening prior to the Endurance Test, within 60 s after, and 10 min after, completion of Phase D (cross-country jumping). The following were determined in the blood samples and compared between the 2 groups of horses: packed cell volume (PCV), serum total protein [TP], serum albumin [ALB], plasma lactate [lactate], serum total calcium [TCa], plasma ionised calcium [Ca+2], serum inorganic phosphate [PO4], plasma pH, plasma sodium [Na], plasma potassium [K], serum chloride [Cl], serum urea nitrogen [SUN], serum creatinine [Cr] and serum glucose concentrations and aspartate aminotransferase (AST) and creatine kinase (CK) activities. The PCV and [Cr] were higher in the TD group and approached significance (P = 0.063 and P = 0.057, respectively). The [TP], [ALB], [Na], glucose concentration and CK, and AST were significantly higher and [Cl] and [PO4] were significantly lower in the TD group after exercise when compared to the HT group. It was deduced from these data that the horses competing in the 3-day-event experienced greater fluid and electrolyte losses, reduced glomerular filtration, higher glycogenolysis and had greater leakage of enzymes from working muscles during competition than horses competing in the horse trial. PMID:8933086

  1. Dose-dependent changes in some haematological parameters during short-term administration of Hibiscus sabdariffa Calyx aqueous extract (Zobo) in Wistar albino rats.

    PubMed

    Adigun, M O; Ogundipe, O D; Anetor, J I; Odetunde, A O

    2006-03-01

    The extract of Hibiscus Sabdariffa L. (MALVACEAE) is popularly consumed and assumed to have haematological benefits, but no scientific investigations are known in the literature to have been conducted to corroborate this claim. The present study was therefore conducted to evaluate the effects of Hibiscus Sabdariffa L. Calyx extract on some haematological parameters (Haemoglobin, haematocrit, total white blood cells and differentials) in rats, with a view to determining its medicinal usefulness in the treatment of anaemia. Proximate analysis on dry matter basis, and mineral clement analysis were carried out on dried calyx of Hibiscus Sabdariffa L. Different doses of aqueous extract of Hibiscus Sabdariffa L calyx extract (200 - 1000 mg/ kg body wt.) were administered orally by intra Ocsophageal cannulation to four groups of six animals (rats) per group for 14 days. Venous blood samples were collected from each animal in all the groups including the control group on days 0 and 14 of the experiment for haematological investigations. Paired stat analysis of day 0 and day 14 results was done for each group using student's T-test. Proximate and mineral analysis of dry calyx confirmed the presence of some nutrients, e.g. protein, mineral elements (potassium) and Vitamin C in the calyx. After 14 days of the extract administration, significant elevations were observed in haematocrit (P = 0.03) and haemoglobin (P = 0.004) in the groups of animals given doses of 200 mg and 400 mg per kg (P < 0.05) while the groups given high doses revealed significant reductions (P 0.031) in the haematocrit but not in haemoglobin. This study suggests that, aqueous extract of Hibiscus Sabdariffa L. calyx used in this experiment had beneficial effects on the red cells at low doses (200 mg-400 mg/kg) which may not be sustained at higher doses. However, the long-term effects and the possible mechanism (s) of action of the extract should be studied before a recommendation could be made. PMID:17209331

  2. Variation in haematological parameters in children less than five years of age with asymptomatic Plasmodium infection: implication for malaria field studies

    PubMed Central

    Gansane, Adama; Ouedraogo, Issa Nebie; Henry, Noelie Bere; Soulama, Issiaka; Ouedraogo, Esperance; Yaro, Jean-Baptiste; Diarra, Amidou; Benjamin, Sombie; Konate, Amadou Tidiani; Tiono, Alfred; Sirima, Sodiomon Bienvenu

    2013-01-01

    During the season of high malaria transmission, most children are infected by Plasmodium, which targets red blood cells (RBCs), affecting haematological parameters. To describe these variations, we examined the haematological profiles of two groups of children living in a malaria-endemic area. A cross-sectional survey was conducted at the peak of the malaria transmission season in a rural area of Burkina Faso. After informed consent and clinical examination, blood samples were obtained from the participants for malaria diagnosis and a full blood count. Of the 414 children included in the analysis, 192 were not infected with Plasmodium, whereas 222 were asymptomatic carriers of Plasmodium infection. The mean age of the infected children was 41.8 months (range of 26.4-57.2) compared to 38.8 months (range of 22.4-55.2) for the control group (p = 0.06). The asymptomatic infected children tended to have a significantly lower mean haemoglobin level (10.8 g/dL vs. 10.4 g/dL; p < 0.001), mean lymphocyte count (4592/µL vs. 5141/µL; p = 0.004), mean platelet count (266 x 103/µL vs. 385 x 103/µL; p < 0.001) and mean RBC count (4.388 x 106/µL vs. 4.158 x 106/µL; p < 0.001) and a higher mean monocyte count (1403/µL vs. 1192/µL; p < 0.001) compared to the control group. Special attention should be applied when interpreting haematological parameters and evaluating immune responses in asymptomatic infected children living in malaria-endemic areas and enrolled in vaccine trials. PMID:23903982

  3. Biochemical and haematological assessment of toxic effects of the leaf ethanol extract of Petroselinum crispum (Mill) Nyman ex A.W. Hill (Parsley) in rats

    PubMed Central

    2013-01-01

    Background Petroselinum crispum, a bright green biennial shrub is widely used traditionally as a food additive and herbal remedies for many ailments. This study therefore aimed to assess the toxic effects of its leaf extract using some biochemical, haematological parameters. Methods The toxic effects were assessed by quantifying liver enzymes such as serum aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total serum protein and liver weight. Effects on haematological parameters were assessed by analysis of parked cell volume (PCV), red blood cell count (RBC), white blood cells (WBC) and haemoglobin (Hb) concentrations. Histopathological studies were done on the liver and kidneys. Results The extract caused significant increase in serum activity of alanine amino transferase and blood urea nitrogen (BUN) levels at the dose of 1000 mg/kg. Other biochemical and haematological parameters were not affected at lower doses. Conversely, the liver weight was not affected after eight weeks of treatment at the dose levels studied. The organs obtained for pathological study, were structurally unchanged under histopathological evaluation at lower doses but inflammatory and necrotic features were observed at doses???1000 mg/kg. Conclusion The results indicate that the leaf ethanol extract of Petroselinum crispum was hepatotoxic and nephrotoxic at continued oral doses equal to or more than 1000 mg/kg, but no obvious toxicity when used at lower doses. Therefore, there should be caution in its administration to avoid overdosing and known interaction with some medications. In addition, the plant should be kept away from pets and domestic animals and should not be cultivated on soil irrigated with waste water due to their ability to bio-accumulate toxic metals. PMID:23557241

  4. Mortality, morbidity, and haematological results from a cohort of long-term workers involved in 1,3-butadiene monomer production.

    PubMed Central

    Cowles, S R; Tsai, S P; Snyder, P J; Ross, C E

    1994-01-01

    A retrospective mortality analysis and prospective morbidity and haematological analyses were performed for Shell Deer Park Manufacturing Complex (DPMC) male employees who worked in jobs with potential exposure to 1,3-butadiene from 1948 to 1989. 614 employees qualified for the mortality study (1948-89), 438 of those were still employed during the period of the morbidity study (1982-9), and 429 of those had haematological data available for analysis. Industrial hygiene data from 1979 to 1992 showed that most butadiene exposures did not exceed 10 ppm (eight-hour time weighted average (8 hour TWA)), and most were below 1 ppm, with an arithmetic mean of 3.5 ppm. 24 deaths occurred during the mortality study period. For all causes of death, the standardised mortality ratio (SMR) was 48 (95% confidence interval (95% CI) = 31-72), and the all cancer SMR was 34 (95% CI = 9-87). There were only two deaths due to lung cancer (SMR 42, 95% CI = 5-151) and none due to lymphohaematopoietic cancer (expected = 1.2). Morbidity (illness absence) events of six days or more for the 438 butadiene employees were compared with the rest of the complex. No cause of morbidity was in excess for this group; the all cause standardised morbidity ratio (SMbR) was 85 (95% CI = 77-93) and the all neoplasms SMbR was 51 (95% CI = 22-100). Haematological results for the 429 with laboratory data were compared with results for the rest of the complex. No significant differences occurred between the two groups and the distributions of results between butadiene and non-butadiene groups were virtually identical. These results suggest that butadiene exposures at concentrations common at DPMC in the past 10-20 years do not pose a health hazard to employees. PMID:8199682

  5. Survey of antifungal prophylaxis and fungal diagnostic tests employed in malignant haematology and haemopoietic stem cell transplantation (HSCT) in Australia and New Zealand.

    PubMed

    van Hal, S J; Gilroy, N M; Morrissey, C O; Worth, L J; Szer, J; Tam, C S; Chen, S C; Thursky, K A; Slavin, M A

    2014-12-01

    This article reports the findings of a survey developed to assess the current use of antifungal prophylaxis among haematology and infectious disease clinicians across Australia and New Zealand, and their alignment with existing consensus guidelines for the use of antifungal agents in the haematology/oncology setting (published 2008). Surveyed clinicians largely followed the current recommendations for prophylaxis in the setting of induction chemotherapy for acute myeloid leukaemia, as well as autologous and low-risk allogeneic haemopoietic stem cell transplantation (HSCT). In keeping with guideline recommendations, posaconazole was the agent used by most centres for high-risk allogeneic HSCT. However, its routine continuation for 75-100 days post-transplantation without de-escalation suggested use beyond those indications described in the 2008 guidelines, namely pre-engraftment neutropenia and graft-versus-host disease. Variations in practice were observed in other settings, such as acute lymphoblastic leukaemia and myelodysplastic syndrome, reflecting the general lack of evidence for antifungal prophylaxis in these patient populations and changing perceptions of risk. With regard to the availability of testing in cases of suspected breakthrough IFD, 40% of centres did not have access to investigative bronchoscopy within 48?h of referral, and results of Aspergillus galactomannan (GM), fungal polymerase chain reaction and therapeutic drug monitoring (TDM) were not available within 48?h in 83%, 90% and 85% of centres respectively. The survey's findings will influence the recommendations provided in the updated 2014 consensus guidelines for the use of antifungal agents in the haematology/oncology setting. PMID:25482740

  6. Haematological and biochemical profile of growing Yankasa rams fed sorghum stover supplemented with graded levels of dried poultry droppings based diets.

    PubMed

    Bello, Abdul Waheed Adeyemi; Tsado, Daniel Nma

    2013-12-15

    This study was designed to determine the haematological and biochemical profiles of growing Yankasa rams fed sorghum stover supplemented with Sun-Dried Poultry Droppings diets (SDPD). Poultry dropping is a good source of protein supplement. Its high nitrogen content suggests feeding it to ruminant would be an excellent avenue to convert nutrients in the waste into animal products. But a major challenge for it utilization is the danger of pathogenic organisms. Sun-drying of the droppings can render the waste free of pathogens. Thirty growing Yankasa rams aged 9-12 months, weighing 11.5-15.5 kg were randomly divided into five groups (3 in each) and assigned to five experimental diets T1-T5 which contained 0, 20, 40, 60 and 80% SDPD. Blood samples were analyzed for haematological and biochemical parameters. Results showed that White Blood Cell (WBC), Haemoglobin (Hb) and Packed Cell Volume (PCV) were significantly influenced by Dried Poultry Droppings based diets (DPD). Their values were WBC, 10.6, 12.9, 9.5, 7.0 and 10.7 L(-1), Hb, 8.6, 9.3, 8.6, 8.4 and 9.7 g dL(-1) and PCV, 22.9, 29.4, 27.1, 23.6 and 21.5%, respectively. Additionally, urea, sodium and total protein were significantly influenced by treatment diet. Their values were urea, 6.1, 6.3, 6.8, 6.9 and 8.1 mg dL(-1), sodium, 102.9, 128.8, 129.2, 130.7 and 130.7 mmol L(-1). total protein, 6.3, 6.5, 7.1, 7.2 and 7.1 g dL(-1). Most haematological and biochemical values obtained were within the normal range for sheep. SDPS diet can satisfactorily supplement sorghum stover without any deleterious effect on the blood chemistry and haematological profile of growing Yankasa rams. PMID:24517007

  7. Malathion-induced changes in the haematological profile, the immune response, and the oxidative/antioxidant status of Cyprinus carpio carpio: protective role of propolis.

    PubMed

    Yonar, Serpil Mi?e; Ural, Mevlüt ?ener; Silici, Sibel; Yonar, M Enis

    2014-04-01

    The present study investigated the potential ameliorative effects of propolis against malathion toxicity in the blood and various tissues of carp. The fish were exposed to sublethal concentrations of malathion (0.5 and 1 mg/L) for 10 days, and propolis (10 mg/kg of fish weight) was simultaneously administered. Blood and tissue (liver, kidney, and gill) samples were collected at the end of the experiment and analysed to determine the haematological profile (red blood cell count, haemoglobin concentration, haematocrit level, and erythrocyte indices: mean corpuscular volume, mean corpuscular haemoglobin, and mean corpuscular haemoglobin concentration), immune response (white blood cell count, oxidative radical production, nitroblue tetrazolium (NBT) activity, total plasma protein and total immunoglobulin levels, and the phagocytic activity), and oxidant/antioxidant status (malondialdehyde and reduced glutathione levels and superoxide dismutase, catalase, and glutathione peroxidase activities) of the fish. The findings of this study demonstrate that malathion has a negative effect on the haematological parameters, immune response, and antioxidant enzyme activities of the fish. However, the administration of propolis ameliorated the malathion-induced toxic effects. PMID:24480596

  8. The use of body condition and haematology to detect widespread threatening processes in sleepy lizards (Tiliqua rugosa) in two agricultural environments.

    PubMed

    Smyth, Anita K; Smee, Elizabeth; Godfrey, Stephanie S; Crowther, Mathew; Phalen, David

    2014-12-01

    Agricultural practices, including habitat alteration and application of agricultural chemicals, can impact wildlife resulting in their decline. Determining which of these practices are contributing to declines is essential if the declines are to be reversed. In this study, the health of two geographically separated sleepy lizard (Tiliqua rugosa) populations was compared between a rangeland environment and cropping environment using linear body size index (LBSI) and haematology. Animals in the cropping site were smaller, suggesting genetic differences as the result of geographical isolation. The animals in the cropping site had a lower LBSI and many were experiencing a regenerative anaemia. The anaemia was postulated to be the cause of the low LBSI. The anaemia appeared to be the result of haemolysis and was likely to be caused by exposure to agricultural chemicals applied in the cropping site but not the rangeland site. Elevated white blood cell counts in lizards in the rangeland site suggested that they were experiencing an inflammatory disease of possible ecological significance. Together, these results demonstrate the value of combining physical and haematological parameters when studying the impact of agricultural practices on wildlife. They also show that reptiles may be useful as sentinel species for livestock and humans. PMID:26064571

  9. Isodicentric (X)(q13) in haematological malignancies: presentation of five new cases, application of fluorescence in situ hybridization (FISH) and review of the literature.

    PubMed

    Dierlamm, J; Michaux, L; Criel, A; Wlodarska, I; Zeller, W; Louwagie, A; Michaux, J L; Mecucci, C; Van den Berghe, H

    1995-12-01

    Idic(X)(q13) represents a rare but recurrent chromosomal abnormality in haematological malignancies. We present five new cases characterized by this particular aberration and review the literature on this subject. The patients were elderly females with a diagnosis of refractory anaemia (1/5), refractory anaemia with ringed sideroblasts (2/5), chronic myelomonocytic leukaemia (1/5), and Philadelphia chromosome-negative chronic myeloid leukaemia (1/5). Three out of the five patients demonstrated an increased proportion of bone marrow ringed sideroblasts. After a follow-up period of 30-57 months all patients but one are alive. Idic(X)(q13) always occurred as the sole chromosomal abnormality, either in one or in two copies. We confirmed the dicentric nature of the aberration by fluorescence in situ hybridization (FISH) on metaphases as well as interphase nuclei using an X-chromosome-specific alpha-satellite probe, and performed chromosome painting to visualize possible additional chromosomal changes involving the X chromosomes. Our findings and the data of 17 previously published cases indicate that idic(X)(q13): (1) may play a significant pathogenetic role in haematological malignancies affecting exclusively females and deriving predominantly from early progenitor cells; (2) is frequently associated with a pathological iron accumulation; (3) indicates a variable prognosis. PMID:8547134

  10. The use of body condition and haematology to detect widespread threatening processes in sleepy lizards (Tiliqua rugosa) in two agricultural environments

    PubMed Central

    Smyth, Anita K.; Smee, Elizabeth; Godfrey, Stephanie S.; Crowther, Mathew; Phalen, David

    2014-01-01

    Agricultural practices, including habitat alteration and application of agricultural chemicals, can impact wildlife resulting in their decline. Determining which of these practices are contributing to declines is essential if the declines are to be reversed. In this study, the health of two geographically separated sleepy lizard (Tiliqua rugosa) populations was compared between a rangeland environment and cropping environment using linear body size index (LBSI) and haematology. Animals in the cropping site were smaller, suggesting genetic differences as the result of geographical isolation. The animals in the cropping site had a lower LBSI and many were experiencing a regenerative anaemia. The anaemia was postulated to be the cause of the low LBSI. The anaemia appeared to be the result of haemolysis and was likely to be caused by exposure to agricultural chemicals applied in the cropping site but not the rangeland site. Elevated white blood cell counts in lizards in the rangeland site suggested that they were experiencing an inflammatory disease of possible ecological significance. Together, these results demonstrate the value of combining physical and haematological parameters when studying the impact of agricultural practices on wildlife. They also show that reptiles may be useful as sentinel species for livestock and humans.

  11. Splenectomy for haematological disorders.

    PubMed

    Jankulovski, N; Antovic, S; Kuzmanovska, B; Mitevski, A

    2014-01-01

    (Full text is available at http://www.manu.edu.mk/prilozi). Splenectomy is therapeutic for a large host of conditions. It is a consequence of expanding the list of disorders and liberalizing the indications for splenectomy in many diseases. Red blood cells disorders: autoimmune hemolytic anemia, hereditary spherocytosis, hemoglobinopathies and thalassemia are prone to splenectomy after failure of medical therapy. A variety of thrombocytopenic disorders are improved by splenectomy, and the most common indication for splenectomy is ITP (idiopathic thrombocytopenic purpura). Splenectomy is successful in reversing hypersplenism in a spectrum of disease called myeloproliferative disorders. Relief of symptoms from splenomegaly is also achieved, but it does not affect the inexorable course of the disorder. The role of splenectomy in white blood cells disorders (leukemias and lymphomas) is only palliative and facilitates chemotherapy. Splenectomy in patients with hemathologic disorders imparts a risk of fulminant and life threatening infection "overwhelming postsplenectomy sepsis" that can be obviated by appropriate treatment. Although splenectomy for hemathologic disorders is only therapeutic and not curative, the relief of symptoms and for some disorders facilitation of chemotherapy leads to better quality of life and longer survival. Key words: Splenectomy, laparoscopic splenectomy, hemathologic dsorders, hereditary spherocytosis, idio-pathic thrombocytopenic purpura, ITP, myeloproliferative disorders, lymphoma, overwhelming posts-plenectomy sepsis. PMID:24798604

  12. Body experience and mental representation of body image in patients with haematological malignancies and cancer as assessed with the Body Grid.

    PubMed

    Weber, C; Bronner, E; Thier, P; Schoeneich, F; Walter, O; Klapp, B F; Kingreen, D

    2001-12-01

    The domain of body image plays a central role in the quality of life of patients with haematological malignancies and metastasized cancer, since the disease itself as well as the enrolled therapies interfere with psychological and bodily well-being. We approached this highly subjective field by using the repertory grid technique and hypothesized that patients would display a restricted body image, focusing on functional aspects of the body. In all, 55 in-patients (27 men, 28 women, M age = 45.7 yrs, N = 46 with haematological malignancies, N = 9 with metastasized cancer), at the time of initial diagnosis, were included in the study and assessed with the Body Grid, an instrument specifically designed by us for the exploration of body image. The data were analysed by principal component analysis (PCA) and construct categorization. Further, 42 chronic tinnitus sufferers (20 male, 22 female, M age = 46.5 yrs) served as a comparison group. Based on the constructs elicited, six construct categories were formulated in the sense of a first attempt of a hierarchical model (emotion, control, activity, strength, function, appearance). The central constructs (373 construct pairs) were assigned to these categories by three inter-raters. The categories appeared in the following order of frequency: function (27.1%), emotion (20.4%), strength (20.1%), activity (15%), control (10.2%) and appearance (7.2%). PCA indicated that the patients mainly demonstrated a restricted view of their body. In the tinnitus group, the most frequent category proved to be activity (21.3%), closely followed by function (21.1%) and control (20.9%). The body image was also restricted (PCA). The restriction of body image, together with the specific construct choice, seen in the haematology and cancer patients reflects the existential threat of the disease and may serve as a coping strategy. The high percentage of emotional constructs may mirror the patients' need for further support. The distinct distribution of construct categories in the two different patient samples supports the applicability of the proposed preliminary model. PMID:11780798

  13. Rise of the mutants: report from the 19th conference of the European Haematology Association, Milan, 12–15 June 2014

    PubMed Central

    Mazzarella, Luca

    2014-01-01

    At the 19th conference of the European Haematology Association in Milan, we saw the true and dramatic changes brought about by the integration of extensive genomic information in clinical practice, and the dilemmas that accompany such a rapid increase in knowledge. Each disease is sliced more and more into smaller pieces, each with its own better-determined outcome and treatment. We also observed the rise of mutant-specific epigenetic agents, which benefit from knowing the underlying genetic abnormality to specifically assign an epigenetic drug where it is needed. In contrast to the ‘one mutation, one drug’ approach, others are pursuing the search for drugs targeting pathways fundamental for the survival of all or most cancer cells, sometimes looking at more ‘exotic’ pathways like neddylation or nuclear export. PMID:25183998

  14. Introduction to the updated Australian and New Zealand consensus guidelines for the use of antifungal agents in the haematology/oncology setting, 2014.

    PubMed

    Slavin, M A; Thursky, K A; Worth, L J; Chang, C C; Morrissey, C O; Blyth, C C; Chen, S C; Szer, J

    2014-12-01

    This article introduces the second revision of the Australian and?New?Zealand consensus guidelines for the use of antifungal agents in the haematology/oncology setting. The current update occurs within the context of a growing population at risk of invasive fungal disease, improved understanding of risk factors, availability of new diagnostic tests, a much-expanded evidence base and changing clinical paradigms. Here, we provide an overview of the history and purpose of the guidelines, including changes in scope since the last clinical update was published in 2008. The process for development, and for enabling review of draft recommendations by end-users and other relevant stakeholders, is described. The approach to assigning levels of evidence and grades of recommendation is also provided, along with a comparison to international grading systems. PMID:25482739

  15. Inhibition of bromodomain and extra-terminal proteins (BET) as a potential therapeutic approach in haematological malignancies: emerging preclinical and clinical evidence

    PubMed Central

    Chaidos, Aristeidis; Caputo, Valentina

    2015-01-01

    Post-translational modifications of the nucleosomal histone proteins orchestrate chromatin organization and gene expression in normal and cancer cells. Among them, the acetylation of N-terminal histone tails represents the fundamental epigenetic mark of open structure chromatin and active gene transcription. The bromodomain and extra-terminal (BET) proteins are epigenetic readers which utilize tandem bromodomains (BRD) modules to recognize and dock themselves on the acetylated lysine tails. The BET proteins act as scaffolds for the recruitment of transcription factors and chromatin organizers required in transcription initiation and elongation. The recent discovery of small molecules capable of blocking their lysine-binding pocket is the first paradigm of successful pharmacological inhibition of epigenetic readers. JQ1 is a prototype benzodiazepine molecule and a specific BET inhibitor with antineoplastic activity both in solid tumours and haematological malignancies. The quinolone I-BET151 and the suitable for clinical development I-BET762 benzodiazepine were introduced in parallel with JQ1 and have also shown potent antitumour activity in preclinical studies. I-BET762 is currently being tested in early phase clinical trials, along with a rapidly growing list of other BET inhibitors. Unlike older epigenetic therapies, the study of BET inhibitors has offered substantial, context-specific, mechanistic insights of their antitumour activity, which will facilitate optimal therapeutic targeting in future. Here, we review the development of this novel class of epigenetic drugs, the biology of BET protein inhibition, the emerging evidence from preclinical work and early phase clinical studies and we discuss their potential role in the treatment of haematological malignancies. PMID:26137204

  16. Mucormycosis in patients with haematological malignancies: a retrospective clinical study of 37 cases. GIMEMA Infection Program (Gruppo Italiano Malattie Ematologiche Maligne dell'Adulto).

    PubMed

    Pagano, L; Ricci, P; Tonso, A; Nosari, A; Cudillo, L; Montillo, M; Cenacchi, A; Pacilli, L; Fabbiano, F; Del Favero, A

    1997-11-01

    A retrospective study of 37 patients with haematological malignancy (21 acute myeloid leukaemia, 11 acute lymphoid leukaemia, two lymphoma, two hairy cell leukaemia, one Hodgkin's disease) and histologically documented mucormycosis was conducted to evaluate the clinical characteristics and ascertain the factors which influenced the outcome from mycotic infection. Patients were admitted to 18 haematology divisions in tertiary care or university hospitals in Italy between 1987 and 1995. Fever, thoracic pain, dyspnoea and cough were the most frequent presenting symptoms. At the onset, 89% patients were neutropenic (neutrophil counts < 0.5 x 10(9)/l) with a median duration of previous neutropenia of 14 d (range 6-60). The most frequent sites of infection were lungs (81%), CNS (27%), sinus (16%), liver (16%) and orbital space (10%). Only three patients were asymptomatic. A correct in vivo diagnosis was made in only 13 (35%) patients. When performed, thoracic and cranial CT scan were the most useful diagnostic investigations. Despite the fact that 26 febrile patients were treated with empirical antifungal treatment, 28 of the 37 patients (76%) died from fungal infection at a median time of 17 d from the onset of clinical symptoms. Nine patients were cured by antifungal therapy plus, in five cases, radical surgery procedures. An analysis of factors influencing outcome demonstrated that the resolution of chemotherapy-induced neutropenia and prolonged treatment with amphotericin B and, if feasible, radical surgical debridement treatment, were significantly correlated with recovery from infection. Mucormycosis, a rare filamentous fungal infection that occurs most frequently in neutropenic acute leukaemia patients, is characterized by a high mortality rate. Extensive and aggressive diagnostic and therapeutic procedures are essential to improve the prognosis in these patients. PMID:9375750

  17. Detection of (1?3)-?-D-glucan in same-day urine and serum samples obtained from patients with haematological malignancies.

    PubMed

    Raggam, Reinhard B; Fischbach, Lara M L; Prattes, Juergen; Duettmann, Wiebke; Eigl, Susanne; Reischies, Frederike; Wölfler, Albert; Rabensteiner, Jasmin; Prueller, Florian; Krause, Robert; Hoenigl, Martin

    2015-07-01

    Serum 1,3-beta-d-glucan (BDG) testing is an established diagnostic marker for invasive fungal infections (IFI) among patients with haematological malignancies. In contrast limited data exist regarding the application of urine BDG testing. Same-day midstream urine and serum screening samples were collected in adult patients with underlying haematological malignancies. A total of 80 urine samples from 46 patients were investigated: Twenty-six had positive corresponding serum BDG >120 pg ml(-1) , 27 intermediate (60-80 pg ml(-1) ), and 27 negative serum BDG (<25 pg ml(-1) ). A significant positive correlation between BDG in serum and urine samples was observed (P = 0.025; r = 0.252). Sensitivity, specificity, positive predictive value and negative predictive value (compared with same-day serum results) were: 42%, 76%, 46%, 73% when using an 80 pg ml(-1) urine cut-off, and 35%, 96%, 82%, 75% for a 250 pg ml(-1) cut-off. Urine BDG seemed to be higher in samples obtained from patients with probable IFI (n = 13, median 145, IQR 22-253) compared to those from patients without IFI (n = 56, median 24, IQR 15-88) but the difference was not significant (P = 0.069). Overall correlation of same-day urine BDG and serum BDG was moderate. However, urine BDG testing may warrant further investigation in larger studies, as high-positive urine results correlated with high-positive corresponding serum levels and clinical performance was comparable to serum BDG. PMID:25959065

  18. Experimental Studies on the Hypolipidemic and Haematological Properties of Aqueous Leaf Extract of Cleistopholis Patens Benth. & Diels. (Annonacae) in Hypercholesterolemic Rats

    PubMed Central

    Udem, SC; Ezeonuegbu, UC; Obidike, RI

    2011-01-01

    Background: Cleistopholis patens (Cp) (Annonacae) is a popular medicinal herb used in the the treatment of cardiovascular disorders in Nigerian ethnomedicine. Objectives: The present study investigated the effects of the aqueous leaf extract of Cp on lipid and haematological profile of hypercholesterolemic Albino rats. Methods: The animals were divided into 5 groups (A – E) of 5 animals per group. All the animals in groups A – D, along with their normal diet, also received 400 mg/kg of cholesterol suspended in 2 % Tween 80 (p.o) daily for 60 days. Six hours after each cholesterol feeding, the animals were treated respectively with a hypolipodemic drug, simvastatin; 10 mg/kg, C. patens extract; 400 mg/kg, 600 mg/kg and 2 % Tween 80 (negative control). The last group (positive control) received cholesterol free diet and 2 % Tween 80. All the treatments were administered orally for a period of 60 days. Thereafter, the lipid and haematological profile of serum and blood samples collected were determined respectively. Results: Treatment with simvastatin (10 mg/kg), the extract (400 and 600 mg/kg) significantly (P =0.033) lowered the total cholesterol, triglycerides, low density lipoproteins (LDL) levels and significantly (P =0.05) increased the level of high density lipoproteins (HDL) in comparison with the negative control group. There was no significant difference between the cholesterol lowering effect of the extract and that of simvastatin (P =0.991). The haemoglobin concentration, packed cell volume, red and white blood cell counts of treated animals did not produce any significant change (P =0.705). The LD50 showed that the extract has a wide margin of safety. Conclusion: The extract showed marked hypolipidemic activity. PMID:23209964

  19. Association of interferon regulatory factor 4 gene polymorphisms rs12203592 and rs872071 with skin cancer and haematological malignancies susceptibility: a meta-analysis of 19 case–control studies

    PubMed Central

    2014-01-01

    Background Research has indicated that the rs12203592 and rs872071 interferon regulatory factor 4 (IRF4) gene polymorphisms correlate with the risk of cancer, especially skin cancer and haematological malignancies, but the results remain controversial. To understand better the effects of these two polymorphisms on skin cancer and haematological malignancies susceptibility, a cumulative meta-analysis was performed. Methods We conducted a search using the PubMed and Web of Science databases for relevant case-control studies published before April 2014. Summary odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were estimated using fixed- or random-effects models where appropriate. Heterogeneity test, publication bias test, and sensitivity analysis were also performed. Results In total, 11 articles comprised of 19 case–control studies were identified; five focused on the rs12203592 polymorphism with 7,992 cases and 8,849 controls, and six were on the rs872071 polymorphism with 3108 cases and 8300 controls. As for rs12203592, a significant correlation with overall skin cancer and haematological malignancies risk was found with the homozygote comparison model (OR?=?1.566, 95% CI 1.087-2.256) and recessive model (OR?=?1.526, 95% CI 1.107-2.104). For rs872071, a significantly elevated haematological malignancies risk was observed in all genetic models (homozygote comparison: OR?=?1.805, 95% CI 1.402-2.323; heterozygote comparison: OR?=?1.427, 95% CI 1.203-1.692; dominant: OR?=?1.556, 95% CI 1.281-1.891; recessive: OR?=?1.432, 95% CI 1.293-1.587; additive: OR?=?1.349, 95% CI 1.201-1.515). Similarly, increased skin cancer and haematological malignancies risk was also identified after stratification of the SNP data by cancer type, ethnicity and source of controls for both polymorphisms. Conclusions Our meta-analysis indicated that the rs12203592 and rs872071 IRF4 gene polymorphisms are associated with individual susceptibility to skin cancer and haematological malignancies. Moreover, the effect of the rs12203592 polymorphism on skin cancer risk was particularly prominent among Caucasians. Further functional research should be performed to validate the association. PMID:24906573

  20. The effect of the combination of acids and tannin in diet on the performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs

    PubMed Central

    2010-01-01

    Background The abolition of in-feed antibiotics or chemotherapeutics as growth promoters have stimulated the swine industry to look for alternatives such as organic acids, botanicals, probiotics and tannin. The objective of the present study was to compare the effects of a combination of acids and tannin with diet with organic acids and diet without growth promoters on the growth performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs. Tannin is more natural and cheaper but possibly with the same effectiveness as organic acids with regard to growth performance. Methods Thirty-six 7 week old grower pigs, divided into three equal groups, were used in a three week feeding trial. Group I was fed basal diet, group II basal diet with added organic acids and group III basal diet with added organic and inorganic acids and tannin. Pigs were weighed before and after feeding and observed daily. Blood was collected before and after the feeding trial for the determination of selected biochemical, haematological and antioxidant enzyme parameters. One-way ANOVA was used to assess any diet related changes of all the parameters. Paired t-test was used to evaluate changes of blood parameters individually in each group of growers before and after feeding. Results No clinical health problems related to diet were noted during the three week feeding trial. The average daily gain (ADG) and selected blood parameters were not affected by the addition to basal diet of either acids and tannin or of organic acids alone. Selected blood parameters remained within the reference range before and after the feeding trial, with the exception of total serum proteins that were below the lower value of reference range at both times. The significant changes (paired t-test) observed in individual groups before and after the feeding trial are related to the growth of pigs. Conclusion Diet with acids and tannin did not improve the growth performance of grower pigs but had no deleterious effects on selected blood parameters. The possibility of beneficial effects of adding acids and tannin in diets on growth performance over a longer period, however, could not be excluded. PMID:20205921

  1. Drug- not carrier-dependent haematological and biochemical changes in a repeated dose study of cyclosporine encapsulated polyester nano- and micro-particles: size does not matter.

    PubMed

    Venkatpurwar, V P; Rhodes, S; Oien, K A; Elliott, M A; Tekwe, C D; Jřrgensen, H G; Kumar, M N V Ravi

    2015-04-01

    Biodegradable nanoparticles are being considered more often as drug carriers to address pharmacokinetic/pharmacodynamic issues, yet nano-product safety has not been systematically proven. In this study, haematological, biochemical and histological parameters were examined on 28 day daily dosing of rats with nano- or micro-particle encapsulated cyclosporine (CsA) to confirm if any changes observed were drug or carrier dependent. CsA encapsulated poly(lactide-co-glycolide) [PLGA] nano- (nCsA) and micro-particles (mCsA) were prepared by emulsion techniques. CsA (15, 30, 45 mg/kg) were administered by oral gavage to Sprague Dawley (SD) rats over 28 days. Haematological and biochemical metrics were followed with tissue histology performed on sacrifice. Whether presented as nCsA or mCsA, 45 mg/kg dose caused significant loss of body weight and lowered food consumption compared to untreated control. Across the doses, both nCsA and mCsA produce significant decreases in lymphocyte numbers compared to controls, commensurate with the proprietary product, Neoral(®) 15. Dosing with nCsA showed higher serum drug levels than mCsA presumably owing to the smaller particle size facilitating absorption. The treatment had no noticeable effects on inflammatory/oxidative stress markers or antioxidant enzyme levels, except an increase in ceruloplasmin (CP) levels for high dose nCsA/mCsA group. Further, only subtle, sub-lethal changes were observed in histology of nCsA/mCsA treated rat organs. Blank (drug-free) particles did not induce changes in the parameters studied. Therefore, it is extremely important that the encapsulated drug in the nano-products is considered when safety of the overall product is assessed rather than relying on just the particle size. This study has addressed some concerns surrounding particulate drug delivery, demonstrating safe delivery of CsA whilst achieving augmented serum concentrations. PMID:25637670

  2. Comparing changes in haematologic parameters occurring in patients included in randomized controlled trials of artesunate-amodiaquine vs single and combination treatments of uncomplicated falciparum in sub-Saharan Africa

    PubMed Central

    2012-01-01

    Background Artesunate-amodiaquine (AS&AQ) is a widely used artemisinin combination therapy (ACT) for falciparum malaria. A comprehensive appreciation of its effects on haematology vs other anti-malarials is needed in view of potential safety liabilities. Methods Individual-patient data analysis conducted on a database from seven randomized controlled trials conducted in sub-Saharan African comparing AS&AQ to reference treatments in uncomplicated falciparum malaria patients of all ages. Haematologic values (white cells total and neutrophil counts, haemoglobin/haematocrit, platelets) were analysed as both continuous and categorical variables for their occurrence, (severity grade 1-4) and changes during follow-up. Risks and trends were calculated using multivariate logistic random effect models. Results 4,502 patients (72% < 5 years old), from 13 sites in nine countries with 28-day follow-up were treated with AS&AQ (45%) or a comparator (other forms of ACT accounted for 27%, other combination 12%, mono-therapies 16%). Pre-treatment leucopaenia (3%) and neutropaenia (6%) were infrequent; anaemia was common (39%). The treatment-emergent adverse events incidence (TEAE = condition not present or less severe pre-treatment) was 11% for neutropaenia, 6% for thrombocytopaenia with AS&AQ and not different from treatment groups; anaemia was higher with AS&AQ (20%) or other forms of ACT (22%) than in non-artemisinin groups (4%, p = 0.001). Multivariate analysis showed that the risk of anaemia, thrombocytopaenia, and leucopaenia decreased with follow-up time, while neutropaenia increased; the risk of anaemia and thrombocytopaenia increased with higher baseline parasitaemia and parasitological reappearance. White cells total count was not a good surrogate for neutropaenia. No systematic significant difference between treatments was detected. Older patients were at lower risks. Conclusion The effects of AS&AQ on haematologic parameters were not different from those of other anti-malarial treatments used in sub-Saharan Africa. This analysis provides the basis for a broader evaluation of haematology following anti-malarial treatment. Continuing monitoring of haematologic safety on larger databases is required. PMID:22276858

  3. [Real-time PCR assay for detection and quantification of human adenoviruses in patients with haematological malignancies and symptoms of lower respiratory tract infection].

    PubMed

    Rynans, Sylwia; Dzieciatkowski, Tomasz; Krenke, Rafa?; Grabczak, Magdalena; Ko?kowska-Le?niak, Agnieszka; Przybylski, Maciej; Sulowsk, Agata; Chazan, Ryszarda; Warzocha, Krzysztof; M?ynarczyk, Grazyna

    2011-01-01

    Human adenoviruses (HAdV) are one of the im-portant infectious etiological factors that affect immunocompromised patients. Because of the large number of HAdV serotypes and their genomic variations, they present a lot of difficulty in laboratory diagnostics. The recent introduction of real-time PCR (qPCR)-based assays has opened new ways to rapid, specific, and highly sensitive HAdV detection. For detection and quantification of HAdV DNA we retrospectively tested serum and bronchoalveolar lavage fluid (BALF) samples obtained from a cohort of 60 adult patients with haematological malignancies presenting clinical and radiological symptoms of lower respiratory tract infections. Human adenoviruses DNA was detected by qPCR method, using primers targeting a conserved region of the adenoviral hexon gene and a specific TaqMan probe. Adenovirus infection occurred with a high incidence in our study group patients. Using qPCR we found that a 21,7% and 15,0% of patients had adenoviral DNA in BALF and serum samples, respectively. The high level of sensitivity, specificity and accuracy provided by real-time PCR assay are favorable for the use in the detection of adenoviral DNA in clinical specimens, especially in immunocompromised patients. PMID:21913485

  4. Measurement of CD4+ T cells in point-of-care settings with the Sysmex pocH-100i haematological analyser

    PubMed Central

    BRIGGS, C; MACHIN, S; MÜLLER, M; HAASE, W; HOFMANN, K; FORSTREUTER, F; HINZMANN, R

    2009-01-01

    The decision to provide antiretroviral therapy to HIV-positive patients mainly depends on the CD4+ T-cell count, with therapy indicated at a cut-off value of <350–200 CD4+ T cells/?l blood. Monitoring patients is still a major problem in countries with limited resources where blood samples often have to be transported over long distances to regional referral centres in which the count can be performed on flow cytometers. We have evaluated a newly developed simple and inexpensive method for CD4+ T-cell quantification. It is a variation of the Invitrogen T4 Quant kit, with manual isolation of nuclei from CD4+ T cells and subsequent counting on the small haematology analyser pocH-100i, Sysmex. We have demonstrated that this new method is highly reproducible and gives stable and linear results over a wide range of CD4+ T-cell concentrations. Method comparison to two different flow cytometers showed excellent correlation with concordances of about 93%. Overall, this method is rapid, easy to perform and offers a good reliable alternative to measurement by flow cytometry. The pocH-100i has the additional benefit of providing a complete blood count with a three-part white blood cell differential and software for patient data storage and handling. PMID:18177434

  5. Haematological and biochemical reference intervals for three species of hydrophiine sea snakes (Hydrophis curtus, H. elegans and H. peronii) in Australia.

    PubMed

    Gillett, Amber K; Flint, Mark; Hulse, Lyndal; Hanger, Jon; Mills, Paul C

    2015-06-01

    This study presents the first set of comprehensive reference intervals (RIs) for plasma biochemistry and haematology for three species of sea snakes common to the Indo-Pacific waters of Australia. In total 98 snakes, composed of Hydrophis curtus (n=?60), H. elegans (n?=?27) and H. peronii (n?=?11), were captured, clinically examined and had venous blood samples collected. All snakes were deemed healthy and in good to excellent body condition with snout to vent lengths of 40.7-73.9?cm (H. curtus), 68.9-131.4?cm (H. elegans) and 55.0-83.0?cm (H. peronii), respectively. Lymphocyte numbers, alkaline phosphatase, alanine aminotransferase, creatine kinase and lactate dehydrogenase levels were species-dependent. All other parameters are presented as a single range for the three species. Gender ratio was evenly distributed for H. curtus and H. elegans, but 8/11 (73%) of H. peronii were males. No significant differences were detected between males and females for any of the measured blood parameters. Lymph contamination was considered and accounted for. Although only three species of sea snakes are represented in this study, the RIs generated may be useful in the clinical assessment of other sea snake species. PMID:25933825

  6. Effects of doxycycline on haematology, blood chemistry and peripheral blood lymphocyte subsets of healthy dogs and dogs naturally infected with Ehrlichia canis.

    PubMed

    Villaescusa, A; García-Sancho, M; Rodríguez-Franco, F; Tesouro, M Á; Sainz, Á

    2015-06-01

    Canine monocytic ehrlichiosis (CME), caused by Ehrlichia canis, is a vector-borne disease with a worldwide distribution. It has been proposed that the pathogenesis, clinical severity and outcome of disease caused by Ehrlichia spp. can be attributed to the immune response rather than to any direct rickettsial effect. Moreover, doxycycline, the antimicrobial of choice for the treatment of CME, has immunomodulatory and anti-inflammatory properties associated with blood leukocyte proliferation function, cytokine synthesis, and matrix metalloproteinase activity. In order to assess the potential effects of doxycycline, dependent and independent of its antimicrobial activity, the present study compared changes in haematology, blood chemistry and circulating lymphocyte subpopulations in 12 healthy dogs and 20 dogs with CME after doxycycline therapy. Some changes were recorded only in the CME affected dogs, probably due to the antimicrobial effect of doxycycline. However, increases in mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, platelet count and ?2-globulins, and decreased plasma creatinine were observed in both healthy and CME affected dogs. The absolute count of B lymphocytes (CD21(+)) increased initially, but then decreased until the end of the study period in both groups. A potential effect of doxycycline unrelated to its antimicrobial activity against E.?canis is suggested, taking into account the results observed both in healthy dogs and in dogs with CME. PMID:25957920

  7. Influence of selected Indian immunostimulant herbs against white spot syndrome virus (WSSV) infection in black tiger shrimp, Penaeus monodon with reference to haematological, biochemical and immunological changes.

    PubMed

    Citarasu, Thavasimuthu; Sivaram, Veeramani; Immanuel, Grasian; Rout, Namita; Murugan, Vadivel

    2006-10-01

    Immunostimulants are the substances, which enhance the non-specific defence mechanism and provide resistance against the invading pathogenic micro-organism. In order to increase the immunity of shrimps against the WSSV, the methanolic extracts of five different herbal medicinal plants like Cyanodon dactylon, Aegle marmelos, Tinospora cordifolia, Picrorhiza kurooa and Eclipta alba were selected and mixed thoroughly in equal proportion. The mixed extract was supplemented with various concentrations viz. 100 (A), 200 (B), 400 (C), and 800 (D) mgkg(-1) through artificial diets individually. The prepared diets (A-D) were fed individually to WSSV free healthy shrimp Penaeus monodon with an average weight of 8.0+/-0.5g for 25 days. Control diet (E), devoid of herbal extract was also fed to shrimps simultaneously. After 25 days of feeding experiment, the shrimps were challenged with WSSV, which were isolated and propagated from the infected crustaceans. The shrimps succumbed to death within 7 days when fed on no herbal immunostimulant diet (E). Among the different concentrations of herbal immunostimulant supplemented diets, the shrimps fed on diet D (800mgkg(-1)) significantly (P<0.0001) had more survival (74%) and reduction in the viral load. Also the better performance of haematological, biochemical and immunological parameters was found in the immunostimulant incorporated diets fed shrimps. The present work revealed that the application of herbal immunostimulants will be effective against shrimp viral pathogenesis and they can be recommended for shrimp culture. PMID:16698283

  8. Clinical utility of Aspergillus galactomannan and PCR in bronchoalveolar lavage fluid for the diagnosis of invasive pulmonary aspergillosis in patients with haematological malignancies.

    PubMed

    Heng, Siow-Chin; Chen, Sharon C-A; Morrissey, C Orla; Thursky, Karin; Manser, Renee L; De Silva, Harini D; Halliday, Catriona L; Seymour, John F; Nation, Roger L; Kong, David C M; Slavin, Monica A

    2014-07-01

    Interpretation of Aspergillus galactomannan (GM) and PCR results in bronchoalveolar lavage (BAL) fluid for the diagnosis of invasive pulmonary aspergillosis (IPA) in patients with haematological malignancies requires clarification. A total of 116 patients underwent BAL for investigation of new lung infiltrates: 40% were neutropenic, 68% and 36% were receiving mould-active antifungal agents and ?-lactam antibiotics. The diagnosis of proven IPA (n = 3), probable IPA (n = 15), and possible invasive fungal disease (IFD, n = 50) was made without inclusion of GM results. BAL GM (at cut-off of 0.8) had lower diagnostic sensitivity for IPA than PCR (61% versus 78%) but higher specificity (93% versus 79%). Both tests had excellent negative predictive values (85-90%), supporting their utility in excluding IPA. The use of BAL GM and PCR results increased the certainty of Aspergillus aetiology in 7 probable IPA cases where fungal hyphae were detected in respiratory samples by microscopy, and upgraded 24 patients from possible IFD to probable IPA. Use of BAL GM and PCR improves the diagnosis of IPA. PMID:24768294

  9. Primary prophylaxis of invasive fungal infections in patients with hematologic malignancies. Recommendations of the Infectious Diseases Working Party of the German Society for Haematology and Oncology

    PubMed Central

    Cornely, Oliver A.; Böhme, Angelika; Buchheidt, Dieter; Einsele, Hermann; Heinz, Werner J.; Karthaus, Meinolf; Krause, Stefan W.; Krüger, William; Maschmeyer, Georg; Penack, Olaf; Ritter, Jörg; Ruhnke, Markus; Sandherr, Michael; Sieniawski, Michal; Vehreschild, Jörg-Janne; Wolf, Hans-Heinrich; Ullmann, Andrew J.

    2009-01-01

    There is no widely accepted standard for antifungal prophylaxis in patients with hematologic malignancies. The Infectious Diseases Working Party of the German Society for Haematology and Oncology assigned a committee of hematologists and infectious disease specialists to develop recommendations. Literature data bases were systematically searched for clinical trials on antifungal prophylaxis. The studies identified were shared within the committee. Data were extracted by two of the authors (OAC and MSi). The consensus process was conducted by email communication. Finally, a review committee discussed the proposed recommendations. After consensus was established the recommendations were finalized. A total of 86 trials were identified including 16,922 patients. Only a few trials yielded significant differences in efficacy. Fluconazole 400 mg/d improved the incidence rates of invasive fungal infections and attributable mortality in allogeneic stem cell recipients. Posaconazole 600 mg/d reduced the incidence of IFI and attributable mortality in allogeneic stem cell recipients with severe graft versus host disease, and in patients with acute myelogenous leukemia or myelodysplastic syndrome additionally reduced overall mortality. Aerosolized liposomal amphotericin B reduced the incidence rate of invasive pulmonary aspergillosis. Posaconazole 600 mg/d is recommended in patients with acute myelogenous leukemia/myelodysplastic syndrome or undergoing allogeneic stem cell recipients with graft versus host disease for the prevention of invasive fungal infections and attributable mortality (Level A I). Fluconazole 400 mg/d is recommended in allogeneic stem cell recipients until development of graft versus host disease only (Level A I). Aerosolized liposomal amphotericin B is recommended during prolonged neutropenia (Level B II). PMID:19066334

  10. Variable presence of KITD816V in clonal haematological non-mast cell lineage diseases associated with systemic mastocytosis (SM-AHNMD).

    PubMed

    Sotlar, Karl; Colak, Sema; Bache, Anja; Berezowska, Sabina; Krokowski, Manuela; Bültmann, Burkhard; Valent, Peter; Horny, Hans-Peter

    2010-04-01

    In a substantial number of patients with systemic mastocytosis (SM), an associated clonal haematological non-mast cell lineage disease (AHNMD) is detectable. Although most of these patients display KIT mutations, especially KIT(D816V), little is known about their exact frequency and their distribution in AHNMD subtypes. We examined 48 patients with SM-AHNMD for the presence of mutant KIT in the SM and AHNMD components of the disease. Mast cells and AHNMD cells were obtained from immunostained bone marrow sections by laser microdissection and examined by melting point analysis of nested-PCR products. KIT(D816V) was found in AHNMD cells in the vast majority of patients with SM-chronic myelomonocytic leukaemia (CMML, 89%). Unexpectedly, KIT(D816V) was far less frequently detectable in AHNMD cells in patients with SM-myeloproliferative neoplasm (MPN, 20%) and SM-acute myeloid leukaemia (AML, 30%). None of the patients with lymphoproliferative AHNMDs displayed KIT codon 816 mutations in AHNMD cells (0/8). In FIP1L1/PDGFRA-positive chronic eosinophilic leukaemia (CEL), neither the SM nor the CEL component of the disease exhibited the KIT mutation. Our findings demonstrate that KIT codon 816 mutations are variably present in AHNMD cells in patients with SM-AHNMD, depending on the subtype of AHNMD. The high frequency of KIT(D816V) in neoplastic mast cells and leukaemic myelomonocytic cells in SM-CMML may point to a common precursor in these patients, and may have implications for the biology of the disease and the development of KIT-targeting therapies. PMID:20112369

  11. Hygienic safety of reusable tap water filters (Germlyser®) with an operating time of 4 or 8 weeks in a haematological oncology transplantation unit

    PubMed Central

    Daeschlein, Georg; Krüger, William H; Selepko, Christian; Rochow, Markus; Dölken, Gottfried; Kramer, Axel

    2007-01-01

    Background Microbial safe tap water is crucial for the safety of immunosuppressed patients. Methods To evaluate the suitability of new, reusable point-of-use filters (Germlyser®, Aquafree GmbH, Hamburg, Germany), three variations of a reusable filter with the same filter principle but with different outlets (with and without silver) and inner surface coating of the filter encasements (with and without nano-crystalline silver) were tested. The filter efficacy was monitored over 1, 4 and 8 weeks operating time in a haematological oncology transplantation unit equipped with 18 water outlets (12 taps, 6 showers). Results The filtered water fulfilled the requirements of absence of pathogens over time. From 348 samples, 8 samples (2.3%) exceeded 100 cfu/ml (no sample ? 500 cfu/ml). As no reprocessed filter exhibited 100% filter efficacy in the final quality control after each reprocessing, these contaminations could be explained by retrograde contamination during use. Conclusion As a consequence of the study, the manufacturer recommends changing filters after 4 weeks in high risk areas and after 8 weeks in moderate infectious risk areas, together with routine weekly alcohol-based surface disinfection and additionally in case of visible contamination. The filter efficacy of the 3 filters types did not differ significantly regarding total bacterial counts. Manual reprocessing proved to be insufficient. Using a validated reprocessing in a washer/disinfector with alkaline, acid treatment and thermic disinfection, the filters were effectively reprocessable and now provide tap water meeting the German drinking water regulations as well as the WHO guidelines, including absence of pathogens. PMID:17521416

  12. Selected haematological and biochemical parameters of blood in rats after subchronic administration of vanadium and/or magnesium in drinking water.

    PubMed

    Scibior, Agnieszka; Zaporowska, Halina; Ostrowski, Jaros?aw

    2006-08-01

    The purpose of these studies was to evaluate the effect of selected vanadium and magnesium doses on certain haematological and biochemical blood parameters in rats. Outbred 2-month-old, albino male Wistar rats received for a period of 6 weeks, as a sole drinking liquid, the following water solutions: group II, sodium metavanadate (SMV) at a concentration of 0.125 mg V/mL; group III, magnesium sulphate (MS) at a concentration of 0.06 mg Mg/mL; and group IV, SMV-MS solution at the same concentrations. The control group received at this time deionized water to drink. It was calculated that group II ingested with drinking water about 10.7 mg V/kg b. w./24 h, group III 6 mg Mg/kg b. w./24 h, and group IV about 9 mg V and 4.5 mg Mg/kg b. w./24 h. The exposure to vanadium alone (group II) led to a statistically significant decrease in body weight gain, food and fluid intakes. Moreover, in the same group of rats a statistically significant decrease in the RBC count, Hb concentration, MCV, and MCH values was demonstrated. Additionally, a statistically significant decrease in the plasma L-ascorbic acid concentration and a significant increase in MDA concentration in blood in this group were found. Instead, after the administration of magnesium alone (group III), a statistically significant decrease in the fluid intake and in the L-ascorbic acid concentration in plasma was noted. Furthermore, in the same group of rats a statistically significant increase in Hb level and in the plasma magnesium concentration was demonstrated. Two-way analysis of variance (ANOVA) did not reveal the interactions between V and Mg. PMID:16783625

  13. In vitro susceptibility to amphotericin B, itraconazole, voriconazole, posaconazole and caspofungin of Aspergillus spp. isolated from patients with haematological malignancies in Tunisia.

    PubMed

    Gheith, Soukeina; Saghrouni, Fatma; Bannour, Wadiaa; Ben Youssef, Yosra; Khelif, Abderrahim; Normand, Anne-Cécile; Piarroux, Renaud; Ben Said, Moncef; Njah, Mansour; Ranque, Stéphane

    2014-01-01

    The resistance of Aspergillus species to antifungal is increasingly reported and the knowledge of the local epidemiology and antifungal susceptibility pattern is pivotal to define adequate treatment policies. Our study aimed to: 1) describe the in vitro antifungal susceptibility profile of the Aspergillus species isolated from patients with haematological malignancies in Tunisia; 2) compare the E-test and Sensititre Yeast-One assays for the detection of paradoxical growth and trailing effect, both phenotypes commonly exhibited by Aspergillus spp. upon exposure to caspofungin and 3) to evaluate the mortality rate in patients according to the causative Aspergillus species and the antifungal treatment. We tested amphotericin B, itraconazole, voriconazole, posaconazole and caspofungin against 48 Aspergillus isolates (17, A. niger; 18, A. flavus; 9, A. tubingensis; 1, A. westerdijkiae; and 1, A. ochraceus) with the E-test. Minimal inhibition concentrations were above the epidemiological cut-off values for amphotericin B in 67% of A. flavus strains; for caspofungin in 22% of A. flavus strains; and for itraconazole in 22% of A. tubingensis strains, voriconazole and posaconazole MICs were below the epidemiological cut-off values for all strains. When exposed to caspofungin, 42% of the strains exhibited trailing effect and 38% paradoxical growth. Trailing effect occurred in 61% of A. flavus strains and paradoxical growth in 62% of Aspergillus section Nigri strains. E-test and Sensititre Yeast-One assays were only fairly concordant for the detection of these phenotypes. Repeatability of both assays was high for trailing effect but poor for paradoxical growth. The relatively high frequency of amphotericin B resistant strains makes voriconazole best adapted as a first-line treatment of invasive aspergillosis from amphotericin B to voriconazole in this hospital. PMID:26034655

  14. The effects of inulin supplementation of diets with or without hydrolysed protein sources on digestibility, faecal characteristics, haematology and immunoglobulins in dogs.

    PubMed

    Verlinden, A; Hesta, M; Hermans, J M; Janssens, G P J

    2006-11-01

    Dogs with food allergy are often treated by giving a diet with hydrolysed protein sources. Prebiotics might also be successful in prevention and treatment of allergic disease through their effect on the colonic microflora, analogous to studies on probiotics in allergic children. The present study was set up to investigate the effect of supplementing inulin (IN) to commercial hypoallergenic dog diets on apparent nutrient digestibility, faecal characteristics, haematology and Ig in dogs. Supplementation of 3 % IN did not affect faecal pH, food and water intake and urine production. Compared with the intact protein diet with a limited number of ingredients (L), the diet with a hydrolysed protein source (H) resulted in an increased water intake (P<0.001), which could be due to the osmotic effect of free amino acids. Faeces production was increased by IN due to increased faecal moisture content. Increased faeces production on the H diet was mainly due to a higher DM excretion. Subsequently, the apparent digestibility coefficient (ADC) of DM was lower in the H diet group. A similar result was noted for ADC of diethyl ether extract and crude ash. The ADC of crude protein was higher in the H diet group, whereas IN decreased the ADC of crude protein. Differences in the ADC of crude protein among the different diets disappeared after correction for a higher faecal biomass, except for the dogs fed the L+IN diet. Total faecal IgA concentrations were lower in the H group (P<0.05) because of lower antigenic stimulation of hydrolysed protein, which implies that hydrolysed protein is really hypoallergenic. The present study indicates that the use of hydrolysed protein diets for canine food allergy treatment can affect digestibility and that combination with IN affected apparent protein digestibility but not IgA response. PMID:17092385

  15. Novel role of triazenes in haematological malignancies: pilot study of Temozolomide, Lomeguatrib and IL-2 in the chemo-immunotherapy of acute leukaemia.

    PubMed

    Caporaso, Patrizia; Turriziani, Mario; Venditti, Adriano; Marchesi, Francesco; Buccisano, Francesco; Tirindelli, Maria Cristina; Alvino, Ester; Garbin, Alberto; Tortorelli, Grazia; Toppo, Laura; Bonmassar, Enzo; D'Atri, Stefania; Amadori, Sergio

    2007-08-01

    Previous studies indicated that dacarbazine and Temozolomide could be highly effective against refractory acute leukaemia. Their activity relies mainly on the generation of methyl adducts at the O(6)-position of guanine in DNA. High levels of O(6)-methylguanine-DNA methyltransferase (MGMT) or a defective mismatch repair (MMR) system, are associated with cellular resistance to triazenes. The MGMT inhibitor, O(6)-(4-bromothenyl)guanine (Lomeguatrib), can restore in vitro sensitivity to Temozolomide in MMR-proficient blasts. In the early 1970s we discovered that, in vivo, triazene compounds induce the appearance of novel transplantation antigens in murine leukaemia ("Chemical Xenogenization", CX). Non-self peptides presented by class I MHC molecules are generated by triazene-induced somatic mutations, affecting retroviral sequences that are detectable in the mouse genome. Moreover, preliminary experiments suggested that human cancer cells can also undergo CX. Therefore, we designed a chemo-immunotherapy strategy in leukaemic patients as follows: (a) cytoreduction and a hypothetical CX phase, i.e. treatment with Lomeguatrib (to suppress MGMT activity) and Temozolomide (to kill sensitive blasts and to presumably induce CX in resistant leukaemic cells); (b) immune response recovery phase using interleukin-2 (to possibly restore an immune response and take advantage of the hypothetical, triazene-induced CX). Here we present the results of pilot study which is in progress in patients with refractory/relapsed acute leukaemia. In all tested cases, Lomeguatrib suppressed MGMT activity in vivo. Six out of eight patients showed partial or complete disappearance of blast cells in peripheral blood or in bone marrow. We observed severe and long-lasting myelosuppression, accompanied by limited non-haematological toxicity. Up to now, two patients are alive (after 9 and 10 months, respectively), four died of opportunistic infections and two of progressive disease. This investigation confirms the potential role of triazenes in leukaemia and highlights the contribution of Lomeguatrib in overcoming drug resistance. Further studies are required to establish whether Temozolomide can induce CX in human leukaemia, and thus offer a new approach to control minimal residual disease. PMID:17500047

  16. Feeding a diet contaminated with ochratoxin A for chickens at the maximum level recommended by the EU for poultry feeds (0.1 mg/kg). 1. Effects on growth and slaughter performance, haematological and serum traits.

    PubMed

    Pozzo, L; Salamano, G; Mellia, E; Gennero, M S; Doglione, L; Cavallarin, L; Tarantola, M; Forneris, G; Schiavone, A

    2013-05-01

    The European Commission Recommendation 2006/576/EC, suggests that the maximum level of Ochratoxin A (OTA) in poultry feeds should be set at 0.1 mg OTA/kg. Thirty-six one-day-old male Hubburd broiler chickens were divided into two groups, a Control (basal diet) and an Ochratoxin A (basal diet + 0.1 mg OTA/kg) group. The growth and slaughter performance traits were recorded. The liver, spleen, bursa of Fabricius and thymus weights were measured. The erythrocyte and leukocyte numbers were assayed in blood samples, and the heterophils to lymphocytes (H/L) ratio was determined. Alpha-1-acid glycoprotein (AGP), lysozyme, the total protein and the electrophoretic pattern were evaluated in serum samples. Liver enzymes (alanino aminotransferase, ALT and aspartate aminotransferase, AST) and kidney function parameters (uric acid and creatinine) were quantified. The results revealed that feeding a 0.1 mg OTA/kg contaminated diet to chicks caused a decrease in the absolute thymus weight (p < 0.05) and a lower total protein (p < 0.01), albumin (p < 0.01), alpha (p < 0.05), beta (p = 0.001) and gamma (p = 0.001) globulins serum concentration in the Ochratoxin A group. Moreover, the albumin-to-globulin (A/G) ratio of the OTA-treated animals resulted to be higher (p < 0.05). Feeding broiler chickens, a diet contaminated with the maximum level admitted by the European Commission Recommendation (0.1 mg OTA/kg), did not affect the animal performance, slaughter traits, organ weights, haematological parameters, liver enzyme or renal function parameters concentrations but had an overall immunosuppressant effect, with reduction in the thymus weight and of the total serum protein, albumin, alpha, beta and gamma globulins concentration. PMID:23639013

  17. One for the road! A study to assess the efficacy of single low-dose regimen of rasburicase in controlling hyperuricaemia in patients with tumour lysis syndrome due to haematological malignancies

    PubMed Central

    Azim, Hamdy A; Bahr, Sherif Ahmed; Kamal, Nermine Shawky; Koura, Mohamed Adel; Tolba, Rehab; Gad, Heba Abdelmoneem; Morsy, Ahmad; Attia, Hossameldin Mohsen; Iskander, Ibraheem; Hammad, Ahmed; Hemed, Mohammed Farouk; Abdallah, Mohammed Fathy; Sadek, Kareem Ahmed; Taha, Alaa Hamdi

    2013-01-01

    We conducted a retrospective audit of six patients with various haematological malignancies (two acute lymphoblastic leukaemia, one acute myeloid leukaemia, and three non-Hodgkin lymphoma); these patients were eligible to receive rasburicase, being at high risk of development of tumour lysis syndrome (TLS). They received a fixed single low-dose regimen of rasburicase (7.5 mg) mainly due to financial restriction, as patients were not supported by the National Health Service and did not have health insurance. We compared uric acid, creatinine levels, and electrolytes (i.e. phosphate, potassium, and calcium) before and after rasburicase administration and also assessed the need for renal replacement therapy after treatment. All six patients had a significant reduction in uric acid levels on the first day, achieving a response rate of 100% (p = 0.008994); creatinine, phosphate, and potassium were reduced significantly as well, with the p values of 0.0439, 0.014326, and 0.002008, respectively; only one patient needed renal replacement therapy in the form of haemodialysis, due to concerns about hyperphosphataemia. Financial difficulties faced either because patients lacked insurance or because of the restricted National Health Service budget in Egypt have resulted in the unavailability of certain modalities of treatment in cancer care and the need to consider more economic yet efficient approaches. Our experience suggests that a single low-dose rasburicase injection (7.5 mg) is an efficient and cost-effective method to control hyperuricaemia in patients with a high risk of developing TLS when compared with the more expensive and extended standard regimen and doses recommended. PMID:24324529

  18. Genetic Disorders in Haematological Practice in India

    Microsoft Academic Search

    D. Mohanty; R. B. Colah; A. C. Gorakshakar; A. H. Nadkarni; S. P. Phanasgaonkar; S. Shetty; K. Ghosh; M. B. Mukherjee

    2002-01-01

    Haemoglobinopathies represent a significant national health burden in India. The distribution of specific disorders varies geographically and by community. Heterozygote frequencies of ?-thalassaemia range from 1 to 15%, resulting in an estimated 20 million carriers. HbS is mainly present in tribal and non-caste communities, with carrier prevalences of up to 40%. By comparison, ?-thalassaemia carriers are found in both the

  19. British Committee for Standards in Haematology

    Microsoft Academic Search

    Y. L. Chee; J. C. Crawford; H. G. Watson; M. Greaves

    Summary Unselected coagulation testing is widely practiced in the process of assessing bleeding risk prior to surgery. This may delay surgery inappropriately and cause unnecessary concern in patients who are found to have 'abnormal' tests. In addition it is associated with a significant cost. This systematic review was performed to determine whether patient bleeding history and unselected coagulation testing predict

  20. Use of radioactive phosphorus in haematology.

    PubMed

    Roberts, B E; Smith, A H

    1997-09-01

    Following the development of the cyclotron in 1932, radio-isotopes became available for use in medicine both as tracer substances and therapeutic agents. The father of nuclear medicine, Dr J. H. Lawrence, pioneered their use in a range of disease states and found that radio-isotopes were of enormous value in the diagnosis and treatment of haemopoetic disease, particularly the myeloproliferative disorders. Radioactive phosphorus 32P emerged as the radio-isotope of choice for the myelosuppressive treatment of myeloproliferative disorders. This article also describes the use of radio-isotopes in the treatment of other disorders: chronic myeloid leukaemia, chronic lymphocytic leukaemia and myeloma, work that is now largely forgotten. All myeloproliferative disorders may evolve without treatment into myelodysplastic syndrome or blast-cell transformation. It is accepted that life is prolonged in myeloproliferative disorders treated with 32P or alkylating agents, yet both are leukaemogenic. The ideal form of treatment for polycythaemia vera is unknown and will remain so, for patients with this disorder often outlive their physician and achieve 90% of normal life expectation. 32P remains the treatment of choice for elderly patients with polycythaemia vera. PMID:9370045

  1. Use of radiactive phosphorus in haematology

    Microsoft Academic Search

    B. E. Roberts; A. H. Smith

    1997-01-01

    Following the development of the cyclotron in 1932, radio-isotopes became available for use in medicine both as tracer substances and therapeutic agents. The father of nuclear medicine, Dr J. H. Lawrence, pioneered their use in a range of disease states and found that radio-isotopes were of enormous value in the diagnosis and treatment of haemopoetic disease, particularly the myeloproliferative disorders.

  2. Original article Early haematological and pathological abnormalities

    E-print Network

    Paris-Sud XI, Université de

    experimentally infected with feline immunodeficiency virus (FIV) F Femenia F Crespeau JJ Fontaine C Boucheix AL intraperitoneally with feline immunodeficiency virus, or with blood from inoculated cats. Three cats of the same age in the lymph nodes, but no viral particles could be observed in them. cat / feline immunodeficiency virus (FIV

  3. Pediatric lupus: varied haematological picture and presentation.

    PubMed

    Thakur, Neha; Chandra, Jagdish; Dhingra, Bhavna; Singh, V

    2015-03-01

    Systemic lupus erythematosus (SLE) is a chronic, multisystem, autoimmune disease characterized by periods of increased disease activity caused by inflammation of blood vessels and connective tissue. Pediatric patients with SLE have a more severe clinical course when compared with adults. Patients commonly present with rash, fever, and arthritis, although the presentation may be unpredictable. Hematological findings are more predominant in children than adults. Thirty-nine percent of children with SLE will develop hematological abnormalities, one of the American Rheumatic Association criteria for classifying the disease. In our case series we found varied hematological picture and presentation. We present here four case reports of SLE cases with interesting hematological features. Our first case is a 13 month old female child who was initially diagnosed as Evans syndrome and 2 years later diagnosed as SLE. Second case is a 3 year old male child who had SLE with warm antibody AIHA. Third case is a 6 year old female child who presented with AIHA and was diagnosed with SLE 6 years later. Fourth case is a 6 year old female child diagnosed as SLE with aplastic anemia. Hematological findings should be carefully assessed and treated in order to decrease disease related morbidity. PMID:25548448

  4. [2013: The Seville document on consensus on the alternatives to allogenic blood transfusion. Update to the Seville document. Spanish Societies of Anaesthesiology (SEDAR), Haematology and Haemotherapy (SEHH), Hospital Pharmacy (SEFH), Critical Care Medicine (SEMICYUC), Thrombosis and Haemostasis (SETH) and Blood Transfusion (SETS)].

    PubMed

    Leal-Noval, S R; Muńoz, M; Asuero, M; Contreras, E; García-Erce, J A; Llau, J V; Moral, V; Páramo, J A; Quintana, M

    2013-01-01

    As allogeneic blood transfusion (ABT) is not harmless, multiple alternatives to TSA (AABT) have emerged, but there is a huge variability with respect to their indications and appropriate use. This variability results from the interplay of a number of factors, which include physicians specialty, knowledge and preferences, degree of anaemia, transfusion policy, and AABT availability. Since the ABBT are not harmless and may not meet costeffectiveness criteria, such avariability is unacceptable. The Spanish Societies of Anaesthesiology (SEDAR), Haematology and Haemotherapy (SEHH), Hospital Pharmacy (SEFH), Critical Care Medicine (SEMICYUC), Thrombosis and Haemostasis (SETH) and Blood Transfusion (SETS) have developed a Consensus Document for the proper use of AABTs. A panel of experts convened by these six Societies have conducted a systematic review of the medical literature and developed the «2013. Seville Document of Consensus on Alternatives to Allogeneic Blood Transfusion», which only considers those AABT aimed to decrease the transfusion of packed red cells. The AABTs are defined as any pharmacological and non-pharmacological measure aimed to decrease the transfusion of of red blood cell concentrates, while preserving the patient safety. For each AABT, the main question is formulated, positively or negatively, as: «Does or does not this particular AABT reduce the transfusion rate?» All the recommendations on the use of AABTs were formulated according to the GRADE (Grades of Recommendation Assessment, Development and Evaluation) methodology. PMID:23789799

  5. Haematological abnormalities in human immunodeficiency virus (HIV) disease.

    PubMed Central

    Costello, C

    1988-01-01

    Peripheral blood and bone marrow changes are commonly seen in disease associated with human immunodeficiency virus (HIV). This annotation aims to summarise these changes and to suggest possible factors entailed in their occurrence. PMID:3045157

  6. Haematological responses of acute nitrite exposure in walleye ( Sander vitreus)

    Microsoft Academic Search

    Barry N. Madison; Yuxiang S. Wang

    2006-01-01

    Nitrite (NO2?) is a toxic intermediary of the bacterial oxidation of nitrogenous wastes (e.g. ammonia) in an aquatic environment. It becomes most lethal when oxygen becomes limited due to high fish densities or in the presence of high bacterial activity due to waste build-up—both situations commonly found in intensive aquaculture. To date however, little is known about how this toxin

  7. Haematological responses of acute nitrite exposure in walleye (Sander vitreus).

    PubMed

    Madison, Barry N; Wang, Yuxiang S

    2006-08-12

    Nitrite (NO2-) is a toxic intermediary of the bacterial oxidation of nitrogenous wastes (e.g. ammonia) in an aquatic environment. It becomes most lethal when oxygen becomes limited due to high fish densities or in the presence of high bacterial activity due to waste build-up-both situations commonly found in intensive aquaculture. To date however, little is known about how this toxin affects the physiology of walleye, an intended culture species, particularly in intensive re-circulating systems. This study aims to define threshold concentrations of nitrite that affect haemoglobin-oxygen affinity and carrying capacity in walleye. During in vivo tests, fish (N=20) were subjected to a medium effective concentration (EC50) of nitrite (0.9 mmol L(-1)) for 48 h while the effects of nitrite accumulation on blood properties were measured. The effects of oxygenation state on red blood cell (RBC) nitrite uptake and metHb formation was further investigated by in vitro tonometry. In vitro nitrite exposure to 3 mmol L(-1) resulted in a significantly higher methaemoglobin formation in 50% air saturated than 100% air saturated RBCs. Both cell water content and haematocrit decreased with time in 50% air saturated treatments, whereas total Hb remained constant, suggesting a reduction in RBC volume. Similar effects were observed during 48 h in vivo and in vitro nitrite exposure tests, indicating the reduction in RBC volume likely was not the result of a catecholamine response. Walleye were found to be tolerant to an accumulation of blood-NO2- levels similar to common carp, a highly Mean Cellular Volume (MCV) tolerant species, before succumbing to methaemoglobinemia. The elevated tolerance to nitrite of walleye is a beneficial characteristic for successful rearing in a culture setting, where reduced oxygen and elevated MCV levels are prevalent. The findings from this study may be used in developing guidelines for species-specific management of nitrogenous wastes in aquaculture. PMID:16806526

  8. Genetic reference materials and their application to haematology

    Microsoft Academic Search

    J. R. Hawkins; M. Hawkins; J. Boyle; E. Gray; P. Matejtschuk; P. Metcalfe

    2010-01-01

    Genetic investigations are becoming increasingly useful and widespread in many areas of human health. However, there is a worldwide lack of certified reference materials for use in genetic testing, meaning that tests are being run without well validated controls and new assays are more difficult to develop and validate. We have responded to this challenge by starting a programme of

  9. Cutaneous mast cell tumor (Mastocytoma): Cyto- histopathological and haematological investigations

    PubMed Central

    2014-01-01

    Cutaneous mast cell tumours (MCTs) are the most common skin tumours in dogs. Due to the prevalence of canine MCTs and the variable biologic behavior of this disease, accurate prognostication and a thorough understanding of MCT biology are critical for the treatment of this disease. A cytologic diagnosis of mast cell tumor with evidence of prior hemorrhage was made, and the masses were surgically removed. Cytological evaluation of fine-needle aspirates from the cutaneous mass from the axillary comprised many well-differentiated, highly granulated mast cells with moderate numbers of eosinophils. Nuclei were varied in size and shape with high nuclear’to’cytoplasmic ratio, prominent nucleoli, marked atypical and mitotic figures. Microscopically, mass consisted of sheets of neoplastic round cells that formed nonencapsulated nodules in the dermis and infiltrated into the adjacent dermal collagen, and also there was diffuse subcutis invasion of round to pleomorphic tumor cells. Tumor cells had moderate to abundant cytoplasm, round to ovoid nuclei with scattered chromatin, and mitotic figures. In this tumor, cytoplasmic granules showed atypical metachromasia. In addition, eosinophils were scattered among the mast cells at the periphery of the nodules. The presence of eosinophils and the observation, at high magnification, of cells with cytoplasmic metachromatic granules. Invasion of the deep subcutaneous fat or cutaneous muscles were a common feature of grade III tumour. Finally, a diagnosis of grade III cutaneous mast cell tumor was made. Virtual slides The virtual slide(s) of this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4755249151157024. PMID:24444100

  10. Predictors of parenting stress in patients with haematological cancer.

    PubMed

    Fernandes, Cheryl; Muller, Robert; Rodin, Gary

    2012-01-01

    This study was designed to identify potential determinants of parenting stress in parents with leukemia or lymphoma and with children younger than age 18 years. Participants recruited at a comprehensive cancer center completed self-report measures of parenting stress, attachment style, parenting alliance, depression, and anxiety. Depression was positively correlated with parenting stress. Anxious and avoidant attachment styles, but not the degree of physical distress or the quality of the parenting alliance, significantly predicted parenting stress. These findings underscore the relationship of parenting stress to psychological well-being and the contribution of individual characteristics in parents to this stress. PMID:22269077

  11. HAEMATOLOGIC PARAMETERS ON VARIOUS SPECIES OF STRIGIFORMES AND FALCONIFORMES

    Microsoft Academic Search

    ELIZABETH E. SMITH

    1978-01-01

    Normal mean values for packed cell volume, total erythrocyte count, total leukocyte count, total protein and mean corpuscular volume were obtained from 37 species of Strigiforines and Falconiformes representing 207 individuals.

  12. Overview of Histone Deacetylase Inhibitors in Haematological Malignancies

    PubMed Central

    Bishton, Mark J.; Johnstone, Ricky W.; Dickinson, Michael; Harrison, Simon; Prince, H. Miles

    2010-01-01

    Histone deacetylase inhibitors (HDACi) can induce hyperacetylation of both histone and non-histone target resulting in epigenetic reprogramming and altered activity, stability and localisation of non-histone proteins to ultimately mediate diverse biological effects on cancer cells and their microenvironment. Clinical trials have demonstrated single agent HDACi to have activity in hematological malignancies, in particular T-cell lymphoma and Hodgkin lymphoma. Combination strategies with standard therapies based on pre-clinical data are being employed with significant success due to their excellent side effect profile. Correlative studies will provide valuable information on the sub-groups of patients more likely to respond or be resistant to HDACi therapy, while long-term monitoring for toxicities is also needed.

  13. Guidelines on product liability for the hospital blood bank. The British Committee for Standards in Haematology.

    PubMed

    1990-01-01

    This report aims to clarify the position of the hospital blood bank in the light of product liability legislation contained in the Consumer Protection Act of 1987. Blood has been defined a 'product' under this Act. The potential for the blood bank to be seen in the role of 'supplier', 'keeper' or even 'producer' in the chain of product supply to the patient is explained and advice given on the resulting implications for blood bank practice. It will be necessary to define, adopt and implement standard operating procedures (SOP) for all blood bank activities. Guidance is given on the format, preparation and content of SOPs and specimen examples offered. The fundamental importance of quality assurance is emphasized. PMID:2272161

  14. NUTRIENT DIGESTIBILITY, HAEMATOLOGICAL AND SERUM BIOCHEMICAL INDICES OF RABBITS FED GRADED LEVELS OF Acacia Albida PODS

    Microsoft Academic Search

    In a 12-week feeding trial, the nutrient digestibility and blood parameters of crossbred (Dutch x New Zealand white) rabbits fed graded levels of Acacia albida pods (AAP) were investigated during weeks 8 and 12 of the study period. The AAP were included at 0, 10, 20, 30 and 40% levels in diets 1, 2, 3, 4 and 5, respectively. The

  15. Effects of Fagonia cretica L. constituents on various haematological parameters in rabbits

    Microsoft Academic Search

    M Asif Saeed; A Wahid Sabir

    2003-01-01

    Effects of powdered Fagonia cretica plant and its two major triterpenoid saponins (saponin-I and saponin-II), isolated from its ethanolic extract, on red blood cells (RBC) count, haemoglobin concentration (HC), mean corpuscular haemoglobin (MCH) and on total leucocyte (WBC) count of normal male rabbits were investigated. Saponins treated three dose groups of animals indicated significant decrease in RBC count during the

  16. Epigenetic inactivation of the hsa-miR-203 in haematological malignancies

    PubMed Central

    Chim, Chor Sang; Wong, Kwan Yeung; Leung, Chung Ying; Chung, Lap Ping; Hui, Pak Kwan; Chan, Sau Yan; Yu, Li

    2011-01-01

    Abstract miR-203 is a tumour suppressor microRNA (miRNA). We studied the methylation of hsa-miR-203 in 150 samples including acute myeloid leukaemia (AML), acute lymphoblastic leukaemia (ALL), chronic myeloid leukaemia (CML), chronic lymphocytic leukaemia (CLL) and non-Hodgkin’s lymphoma (NHL) by methylation-specific PCR, and miRNA expression by stem-loop RT-qPCR. hsa-miR-203 promoter was unmethylated in normal controls but homozygously methylated in two AML and four lymphoma cell lines, in which 5-Aza-2?-deoxycytidine treatment led to promoter demethylation and miR-203 re-expression. Restoration of miR-203 expression in lymphoma cells inhibited cellular proliferation and increased cell death, suggesting an inherent tumour suppressor activity. In primary samples, hsa-miR-203 methylation was absent in CML but detected in 5.0% ALL, 10.0% AML, 42.0% CLL and 38.8% of NHL (including six [60.0%] natural killer-cell, nine [40.9%] B-cell and four [23.5%] T cell NHL). Moreover, hsa-miR-203 methylation was associated with hypermethylation of hsa-miR-34a, -124a and -196b in NHL but not CLL. In CLL, hsa-miR-203 methylation was associated with a higher presenting Hb level (P = 0.033). The projected 10 year overall survival of the CLL patients was 58.2%, which was impacted by Rai stage and high-risk karyotypes but not hsa-miR-203 methylation. hsa-miR-203 was more frequently methylated in lymphoid than myeloid malignancies (P = 0.002). In conclusion, miR-203, a tumour suppressor gene, was hypermethylated in a tumour-specific manner with gene silencing. hsa-miR-203 was more frequently hypermethylated in lymphoid than myeloid malignancies. In NHL, hsa-miR-203 methylation was associated with concomitant methylation of other tumour suppressor miRNAs. The frequent hsa-miR-203 methylation in lymphoid malignancies suggested a pathogenetic role of hsa-miR-203 methylation. PMID:21323860

  17. Hepatic respiratory compensation and haematological changes in the cave cyprinid, Phreatichthys andruzzii

    Microsoft Academic Search

    Giuliano Frangioni; Roberto Berti; Gianfranco Borgioli

    1997-01-01

    In several ectotherms, including all members of the Osteichthyes studied so far, the spleen is capable of storing and releasing\\u000a erythrocytes according to the animal's respiratory needs. The tropical cave cyprinid Phreatichthys andruzzii uses its liver rather than the spleen as the site of accumulation in the respiratory compensation process, like the amphibian\\u000a Rana esculenta. The reversible process of erythrocyte

  18. Haematologic and growth response to prepartum administration of vitamin A in calves.

    PubMed

    Salam Abdullah, A; Vijchulata, P; Sivarajasingam, S; Ragavan, K

    1987-01-01

    Injectable vitamin A was given to six pregnant beef cows in their last third of pregnancy to study the effect of this vitamin in their calves. Average birth weight and growth rate of calves from the treated cows were higher than that of calves from the nontreated cows. Prepartum vitamin A injections also resulted in a significant increase (P less than 0.05) in the mean corpuscular volume (MCV), total serum protein and globulin fraction of serum protein in calves of treated cows. PMID:3678931

  19. Haematology and Plasma Biochemistry of Wild Black Flying-Foxes, (Pteropus alecto) in Queensland, Australia.

    PubMed

    McMichael, Lee; Edson, Daniel; McLaughlin, Amanda; Mayer, David; Kopp, Steven; Meers, Joanne; Field, Hume

    2015-01-01

    This paper establishes reference ranges for hematologic and plasma biochemistry values in wild Black flying-foxes (Pteropus alecto) captured in South East Queensland, Australia. Values were found to be consistent with those of other Pteropus species. Four hundred and forty-seven animals were sampled over 12 months and significant differences were found between age, sex, reproductive and body condition cohorts in the sample population. Mean values for each cohort fell within the determined normal adult reference range, with the exception of elevated levels of alkaline phosphatase in juvenile animals. Hematologic and biochemistry parameters of injured animals showed little or no deviation from the normal reference values for minor injuries, while two animals with more severe injury or abscessation showed leucocytosis, anaemia, thrombocytosis, hyperglobulinemia and hypoalbuminemia. PMID:25938493

  20. Oncogene expression in primary myelodysplasia: correlation with haematological, karyotypic, and clinical progression.

    PubMed Central

    Hutchinson, R. M.; Pringle, J. H.; Knight, S. C.; Lauder, I.; Potter, A.; Jagger, C.

    1992-01-01

    AIMS: To see if the relative expressions of proto-oncogenes that are increased in acute myeloid leukaemia are raised in patients with myelodysplastic syndromes (MDS), and to see if they increase with progression to leukaemia. To note if there is a correlation between morphology, karyotype, and these proto-oncogene expressions and if any one proto-oncogene can predict prognosis. METHOD: Bone marrow from 130 patients was analysed at six monthly intervals over two years for relative mRNA expression of seven oncogenes, karyotype, and morphology. The technique used slot blot hybridisation and densitometric analysis. The results were compared with 14 surgical controls and 30 people with vitamin deficiency anaemia. RESULTS: Six of seven oncogenes showed increased expression which progressed with time, but did not correlate with morphological or karyotypic changes. Expression of four of the seven oncogenes was increased in megaloblastic and iron deficiency anaemia. C-mos showed differences among the five morphological subgroups; it correlated with abnormal location (p = 0.025) and seemed to influence prognosis. CONCLUSION: Increased proto-oncogenes reflect the overall marrow perturbation in MDS. C-mos may reflect persistence of monocyte pathway which confirms marrow stability. PMID:1577972

  1. Some selected haematological indices in Wistar rats in the vanadium-ethanol interaction.

    PubMed

    Zaporowska, H; Wasilewski, W

    1990-01-01

    1. Wistar rats of both sexes daily received an ethanol solution of ammonium metavanadate (AMV) of 0.3 mg V/cm3 5% ethanol concentration as sole drinking liquid, for a period of 4 weeks. 2. The reference groups received for drinking aqueous AMV solution, or 5% ethanol, or water. 3. In animals drinking both water and ethanol AMV solution a decrease in the erythrocyte count and haemoglobin level was noted together with an increase of the percentage of reticulocytes and polychromatophilic erythrocytes in the peripheral blood. 4. A small rise of the percentage of polychromatophilic and orthochromatic erythroblasts was at the same time noted in the bone marrow of animals receiving ethanol AMV solution. 5. In the group of animals drinking 5% ethanol a fall of the erythrocyte count was observed and a rise of the leukocyte count, particularly of lymphocytes. 6. Substitution of water by 5% ethanol solution as solvent for AMV did not have any distinct influence on the toxicity of the tested compound. PMID:1980881

  2. Oral and Intravenous Itraconazole for Systemic Fungal Infections in Neutropenic Haematological Patients: Meeting Report

    Microsoft Academic Search

    H. G. Prentice; D. Caillot; B. Dupont; F. Menichetti; U. Schuler

    1999-01-01

    Effective prevention, or treatment, of invasive fungal infection in the neutropenic patient has hitherto been unsatisfactory because of either an inadequate anti-fungal spectrum of the agent or important toxicity. Itraconazole is effective against a broad spectrum of the opportunistic pathogens seen in Europe and North America. Prior problems with absorption, e.g. in the marrow transplant recipient, have been overcome with

  3. Screening for adenoviruses in haematological neoplasia: High prevalence in mantle cell lymphoma.

    PubMed

    Kosulin, Karin; Rauch, Margit; Ambros, Peter F; Pötschger, Ulrike; Chott, Andreas; Jäger, Ulrich; Drach, Johannes; Nader, Alexander; Lion, Thomas

    2014-02-01

    Human adenoviruses possess oncogenic capacity which is well documented in mammalian animal models, but their possible implication in human malignancy has remained enigmatic. Following primary infection, adenoviruses can persist in a latent state in lymphocytes where the virus is apparently able to evade immune surveillance. In the present study, we have employed a broad-spectrum adenovirus polymerase chain reaction (PCR) assay to systematically screen more than 200 diagnostic specimens of different lymphoid malignancies including acute lymphocytic leukaemia (n=50), chronic lymphocytic leukaemia (n=50), various types of malignant lymphoma (n=100) and multiple myeloma (n=11) for the presence of adenoviral sequences. While most entities analysed revealed negative findings in virtually all specimens tested, adenoviral DNA was detected in 15/36 (42%) mantle cell lymphomas investigated. The most prevalent adenoviral species detected was C, and less commonly B. Adenovirus-positive findings in patients with mantle cell lymphoma were made at different sites including bone marrow (n=7), intestine (n=5), lymph nodes (n=2) and tonsillar tissue (n=1). The presence of adenoviral sequences identified by PCR was confirmed in individual cells by fluorescence in-situ hybridisation (FISH). The frequent observation of adenoviruses in mantle cell lymphoma is intriguings, and raises questions about their possible involvement in the pathogenesis of this lymphoid malignancy. PMID:24246703

  4. Cobalamin deficiency resulting in a rare haematological disorder: a case report

    PubMed Central

    2009-01-01

    Introduction We present the case of a patient with a cobalamin deficiency resulting in pancytopaenia, emphasizing the importance to define, diagnose and treat cobalamin deficiency. Case presentation A 52-year-old man from the Democratic Republic of Congo presented to the emergency department with shortness of breath and a sore tongue. Physical examination was unremarkable. His haemoglobin was low and the peripheral blood smear revealed pancytopaenia with a thrombotic microangiopathy. The findings were low cobalamin and folate levels, and high homocysteine and methylmalonate levels. Pernicious anaemia with chronic atrophic gastritis was confirmed by gastric biopsy and positive antiparietal cell and anti-intrinsic factor antibodies. Cobalamin with added folate was given. Six months later, the patient was asymptomatic. Conclusion Cobalamin deficiency should always be ruled out in a patient with pancytopaenia. Our case report highlights a life-threatening cobalamin deficiency completely reversible after treatment. PMID:19946556

  5. [The role of the nurse and child health nurse in a haematology day hospital].

    PubMed

    Perray, Stéphanie; Costes, Élodie; Héritier, Sébastien

    2015-01-01

    The paediatric haemato-oncology nurse and the child health nurse in a day hospital have specific technical skills and in-depth knowledge in order to be able to provide global care for children with leukaemia. Close collaboration between the different professionals working in this care unit is essential. PMID:26183096

  6. Observing a fictitious stressful event: haematological changes, including circulating leukocyte activation.

    PubMed

    Mian, Rubina; Shelton-Rayner, Graham; Harkin, Brendan; Williams, Paul

    2003-03-01

    The aim of this study was to assess the effect of watching a psychological stressful event on the activation of leukocytes in healthy human volunteers. Blood samples were obtained from 32 healthy male and female subjects aged between 20 and 26 years before, during and after either watching an 83-minute horror film that none of the subjects had previously seen (The Texas Chainsaw Massacre, 1974) or by sitting quietly in a room (control group). Total differential cell counts, leukocyte activation as measured by the nitroblue tetrazolium (NBT) test, heart rate and blood pressure (BP) measurements were taken at defined time points. There were significant increases in peripheral circulating leukocytes, the number of activated circulating leukocytes, haemoglobin (Hb) concentration and haematocrit (Hct) in response to the stressor. These were accompanied by significant increases in heart rate, systolic and diastolic BP (P<0.05 from baseline). This is the first reported study on the effects of observing a psychologically stressful, albeit fictitious event on circulating leukocyte numbers and the state of leukocyte activation as determined by the nitrotetrazolium test. PMID:12637206

  7. Our recommendations for avoiding exposure to fungi outside the hospital for patients with haematological cancers.

    PubMed

    Ariza-Heredia, Ella J; Kontoyiannis, Dimitrios P

    2014-06-01

    Despite several chemotherapeutic and preventative advances, opportunistic fungal infections remain common unintended consequences of cancer treatment. Currently, cancer patients spend most of their time between treatments at home, where they can inadvertently come across potential hazards from environmental and food sources. Therefore, infection prevention measures are of the utmost importance for these patients. Although clinicians closely observe patients throughout their treatment courses in the hospital, the focus of clinical visits is predominantly on cancer care, and clinicians seldom provide recommendations for prevention of such infections. Herein, we provide practical recommendations for busy clinicians to help them educate patients regarding potential sources of fungal infections outside the hospital. PMID:24446760

  8. Guidelines for the investigation of invasive fungal infections in haematological malignancy and solid organ transplantation

    Microsoft Academic Search

    D. W. Denning; E. G. V. Evans; C. C. Kibbler; M. D. Richardson; M. M. Roberts; T. R. Rogers; D. W. Warnock; R. E. Warren

    1997-01-01

    Invasive fungal infections are increasing in incidence and now affect as many as 50% of neutropenic\\/bone marrow transplant\\u000a patients and 5 to 20% of solid organ transplant recipients. Unfortunately, many of the diagnostic tests available have a low\\u000a sensitivity. The guidelines presented here have been produced by a working party of the British Society for Medical Mycology\\u000a in an attempt

  9. Consensus guidelines for antifungal prophylaxis in haematological malignancy and haemopoietic stem cell transplantation, 2014.

    PubMed

    Fleming, S; Yannakou, C K; Haeusler, G M; Clark, J; Grigg, A; Heath, C H; Bajel, A; van Hal, S J; Chen, S C; Milliken, S T; Morrissey, C O; Tam, C S; Szer, J; Weinkove, R; Slavin, M A

    2014-12-01

    There is a strong argument for the use of antifungal prophylaxis in high-risk patients given the significant mortality associated with invasive fungal disease, the late identification of these infections, and the availability of safe and well-tolerated prophylactic medications. Clinical decisions about which patients should receive prophylaxis and choice of antifungal agent should be guided by risk stratification, knowledge of local fungal epidemiology, the efficacy and tolerability profile of available agents, and estimates such as number needed to treat and number needed to harm. There have been substantial changes in practice since the 2008 guidelines were published. These include the availability of new medications and/or formulations, and a focus on refining and simplifying patient risk stratification. Used in context, these guidelines aim to assist clinicians in providing optimal preventive care to this vulnerable patient demographic. PMID:25482741

  10. Haematological findings in healthy and sick African grey parrots (Psittacus erithacus)

    Microsoft Academic Search

    CM Hawkey; MG Hart; JA Knight; JH Samour; DM Jones

    1982-01-01

    Full blood counts and fibrinogen estimations were carried out on 11 clinically normal, adult African grey parrots. Reference values derived from these tests were used to identify abnormalities in the blood counts of 11 sick parrots thought to be suffering from bacterial infections. All sick birds showed higher than normal heterophil counts, paralleled in some cases by lymphocytosis, monocytosis, thrombocytosis

  11. Viral purging of haematological autografts: should we sneeze on the graft?

    Microsoft Academic Search

    C M Thirukkumaran; J A Russell; D A Stewart; D G Morris

    2007-01-01

    High-dose cytotoxic chemotherapy followed by autologous haematopoietic stem cell transplantation (ASCT) is extensively used for the treatment of many haematopoietic, as well as several epithelial cancers. Disease relapse may be the result of tumour contamination within autograft as evidenced by gene marking studies. The multiple purging strategies that have been described to date have not proven effective in most ASCT

  12. The Use of Hirudin as Universal Anticoagulant in Haematology, Clinical Chemistry and Blood Grouping

    Microsoft Academic Search

    Hans D. Menssen; Karl Melber; Natascha Brandt; Eckhard Thiel

    2001-01-01

    RheinBiotech GmbH Düsseldorf (K.M.), Düsseldorf, Ger- many Undesirable interactions between anticoagulants and diagnostic test kit procedures so far have prevented the development of a single uniform blood sampling tube. Contrary to K2-EDTA, heparin and other antico- agulants, hirudin only minimally alters blood cells and dissolved blood constituents, thus qualifying as a uni- versal anticoagulant for diagnostic purposes. Auto- mated complete

  13. Haematology and Plasma Biochemistry of Wild Black Flying-Foxes, (Pteropus alecto) in Queensland, Australia

    PubMed Central

    McMichael, Lee; Edson, Daniel; McLaughlin, Amanda; Mayer, David; Kopp, Steven; Meers, Joanne; Field, Hume

    2015-01-01

    This paper establishes reference ranges for hematologic and plasma biochemistry values in wild Black flying-foxes (Pteropus alecto) captured in South East Queensland, Australia. Values were found to be consistent with those of other Pteropus species. Four hundred and forty-seven animals were sampled over 12 months and significant differences were found between age, sex, reproductive and body condition cohorts in the sample population. Mean values for each cohort fell within the determined normal adult reference range, with the exception of elevated levels of alkaline phosphatase in juvenile animals. Hematologic and biochemistry parameters of injured animals showed little or no deviation from the normal reference values for minor injuries, while two animals with more severe injury or abscessation showed leucocytosis, anaemia, thrombocytosis, hyperglobulinemia and hypoalbuminemia. PMID:25938493

  14. Influence of Ascorbic Acid Supplementation on the Haematological and Clinical Biochemistry Parameters of Male Rabbits Exposed to Aflatoxin B1

    Microsoft Academic Search

    M. I. Yousef; M. H. Salem; K. I. Kamel; G. A. Hassan

    2003-01-01

    This study was undertaken to evaluate the effectiveness of L?ascorbic acid (AA) in alleviating the toxicity of aflatoxin B1 (AFB1) in male New?Zealand white rabbits. Five rabbits (6 months of age and mean body weight 3.12 kg) per group were assigned to 1 of 6 treatment groups: 0 mg AA and 0 mg AFB1\\/kg BW (control); 20 mg AA\\/kg BW;

  15. Effects of the aqueous extract of fresh leaves of Calotropis procera on haematological and biochemical parameters in female rabbits

    Microsoft Academic Search

    Jato Denis Mbako; Zainab Adamu; Jacob Kotei Afutu; Atiku Aliyu; Shamaki David; M. B. Umar; Chudy Nduaka

    Calotropis procera has been reported to be medicinal and toxic in animals. A toxicological evaluation of the aqueous extract of its fresh leaves was conducted in female rabbits (Oryctolagus cuniculus). Low levels of phytochemicals (alkaloids, saponins, tannins, cardiac glycosides, and flavonoids) were found while 0.23, 0.03, 0.82 and 9.5 mg\\/g of iron, lead, sodium and potassium, respectively, were detected. Acute

  16. Spatial variation in mortality risk for haematological malignancies near a petrochemical refinery: a population-based case-control study

    PubMed Central

    Di Salvo, Francesca; Meneghini, Elisabetta; Vieira, Veronica; Baili, Paolo; Mariottini, Mauro; Baldini, Marco; Micheli, Andrea; Sant, Milena

    2015-01-01

    Introduction The study investigated the geographic variation of mortality risk for hematological malignancies (HMs) in order to identify potential high-risk areas near an Italian petrochemical refinery. Material and methods A population-based case-control study was conducted and residential histories for 171 cases and 338 sex- and age-matched controls were collected. Confounding factors were obtained from interviews with consenting relatives for 109 HM deaths and 267 controls. To produce risk mortality maps, two different approaches were applied. We mapped (1) adptive kernel density relative risk estimation (KDE) for case-control studies which estimates a spatial relative risk function using the ratio between cases and controls’ densities, and (2) estimated odds ratios for case-control study data using generalized additive models (GAMs) to smooth the effect of location, a proxy for exposure, while adjusting for confounding variables. Results No high-risk areas for HM mortality were identified among all subjects (men and women combined), by applying both approaches. Using the adaptive KDE approach, we found a significant increase in death risk only among women in a large area 2–6 km southeast of the refinery and the application of GAMs also identified a similarly-located significant high-risk area among women only (global p-value<0.025). Potential confounding risk factors we considered in the GAM did not alter the results. Conclusion Both approaches identified a high-risk area close to the refinery among women only. Those spatial methods are useful tools for public policy management to determine priority areas for intervention. Our findings suggest several directions for further research in order to identify other potential environmental exposures that may be assessed in forthcoming studies based on detailed exposure modeling. PMID:26073202

  17. Influence of granite-grit on nutrient digestibility and haematological parameters of broiler chickens fed rice offal based diets.

    PubMed

    Idachaba, C U; Abeke, F O; Olugbemi, T S; Ademu, L A

    2013-10-01

    A total of 270 broiler chickens were used for the study. The birds were fed common diet containing 23% Crude protein and 2864 kcal kg(-1) Metabolizable energy at the starter phase while 20% Crude protein and 2923 kcal kg(-1) Metabolizable energy was fed at the finisher phase. Starter and finisher diets contained 10 and 15% inclusion levels of rice offal respectively. Granite grit was added to the basal diet at 0.0, 2.0, 4.0, 6.0, 8.0 and 10.0 g per bird per month thus making a total of six treatments. Each treatment was replicated three times with 15 birds per replicate in a completely randomized design. Packed cell volume and haemoglobin level were not significantly (p>0.05) affected by dietary grit levels while total protein increased across the graded levels of granite grit. Crude protein, crude fibre and nitrogen free extract significantly (p<0.05) improved with increasing grit levels. These parameters improved up to the highest level of grit addition (10.0 g) granite-grit. It was concluded that 10.0 g granite grit per bird per month is beneficial to broiler chickens as it allows for efficient nutrient utilization. Further study to determine the optimum level of granite grit in broiler diet is encouraged since result obtained showed the optimum level was not attained. PMID:24502172

  18. Cardiorespiratory, hormonal and haematological responses to submaximal cycling performed 2 days after eccentric or concentric exercise bouts

    Microsoft Academic Search

    Michael Gleeson; Andrew K. Blannin; Bin Zhu; Stephen Brooks; Robert Cave

    1995-01-01

    Eccentric muscle actions are known to induce delayed?onset muscle soreness (DOMS) and muscle weakness (reduced static strength and dynamic peak power output) that may persist for several days. The aim of the present study was to determine whether DOMS?inducing exercise affects physiological responses to subsequent submaximal dynamic exercise. Physiological and metabolic responses to a standardized exercise task were measured 2

  19. Haematological and phenoloxidase activity changes in the shrimp Penaeus stylirostris in relation with the moult cycle: protection against vibriosis

    Microsoft Academic Search

    GILLES LE MOULLAC; MARC LE GROUMELLEC; DOMINIQUE ANSQUER; SÉBASTIEN FROISSARD; PEVA LEVY

    1997-01-01

    This paper describes the changes in the total and differential haemocyte counts during the moult cycle of the shrimp,Penaeus stylirostris. Correlative measures of phenoloxidase (PO) activity and resistance to infection against vibriosis were also investigated during the moult cycle. Three types of cells are identified: hyaline cells (HC), which are small with a big nucleus, represent 80% of the total;

  20. Integrated Haematological Profiles of Redox Status, Lipid, and Inflammatory Protein Biomarkers in Benign Obesity and Unhealthy Obesity with Metabolic Syndrome

    PubMed Central

    Lubrano, Carla; Valacchi, Giuseppe; Specchia, Palma; Gnessi, Lucio; Rubanenko, Elizaveta P.; Shuginina, Elena A.; Trukhanov, Arseny I.; Korkina, Liudmila G.; De Luca, Chiara

    2015-01-01

    The pathogenesis of obesity (OB) and metabolic syndrome (MetS) implies free radical-, oxidized lipid- (LOOH-), and inflammatory cytokine-mediated altered pathways in target organs. Key elements of the transition from benign OB to unhealthy OB+MetS remain unclear. Here, we measured a panel of redox, antioxidant, and inflammation markers in the groups of OB patients (67 with, 45 without MetS) and 90 controls. Both OB groups displayed elevated levels of adipokines and heavy oxidative stress (OS) evidenced by reduced levels of glutathione, downregulated glutathione-S-transferase, increased 4-hydroxynonenal-protein adducts, reactive oxygen species, and membrane-bound monounsaturated fatty acids (MUFA). Exclusively in OB+MetS, higher-than-normal glutathione peroxidase activity, tumor necrosis factor-?, and other proinflammatory cytokines/chemokines/growth factors were observed; a combination of high adipokine plasminogen activator inhibitor-1 and MUFA was consistent with increased cardiovascular risk. The uncomplicated OB group showed features of adaptation to OS such as decreased levels of vitamin E, activated superoxide dismutase, and inhibited catalase, suggesting H2O2 hyperproduction. Proinflammatory cytokine pattern was normal, except few markers like RANTES, a suitable candidate for therapeutic approaches to prevent a setting of MetS by inhibition of LOOH-primed leukocyte chemotaxis/recruitment to target tissues.

  1. Folic acid supplemented goat milk has beneficial effects on hepatic physiology, haematological status and antioxidant defence during chronic Fe repletion.

    PubMed

    Alférez, María J M; Rivas, Emilio; Díaz-Castro, Javier; Hijano, Silvia; Nestares, Teresa; Moreno, Miguel; Campos, Margarita S; Serrano-Reina, Jose A; López-Aliaga, Inmaculada

    2015-02-01

    The aim of the current study was to asses the effect of goat or cow milk-based diets, either normal or Fe-overloaded and folic acid supplement on some aspects of hepatic physiology, enzymatic antioxidant defence and lipid peroxidation in liver, brain and erythrocyte of control and anaemic rats after chronic Fe repletion. 160 male Wistar rats were placed on 40 d in two groups, a control group receiving normal-Fe diet and the Fe-deficient group receiving low Fe diet. Lately, the rats were fed with goat and cow milk-based diets during 30 d, with normal-Fe content or Fe-overload and either with normal folic or folic acid supplemented. Fe-overload increased plasma alanine transaminase and aspartate transaminase levels when cow milk was supplied. Dietary folate supplementation reduced plasma transaminases levels in animals fed goat milk with chronic Fe overload. A remarkable increase in the superoxide dismutase activity was observed in the animals fed cow milk. Dietary folate supplement lead to a decrease on the activity of this enzyme in all the tissues studied with both milk-based diets. A concomitant increment in catalase was also observed. The increase in lipid peroxidation products levels in rats fed cow milk with Fe-overload, suggest an imbalance in the functioning of the enzymatic antioxidant defence. In conclusion, dietary folate-supplemented goat milk reduces both plasma transaminases levels, suggesting a hepatoprotective effect and has beneficial effects in situation of Fe-overload, improving the antioxidant enzymes activities and reducing lipid peroxidation. PMID:25394837

  2. Case-control study of multiple chemical sensitivity, comparing haematology, biochemistry, vitamins and serum volatile organic compound measures

    Microsoft Academic Search

    Cornelia Johanna Baines; Gail Elizabeth; Nicole Riley; David Edward; C. Cole; Lynn Marshall; Barry Loescher; Vartouhi Jazmaji

    Background Multiple chemical sensitivity (MCS), although poorly understood, is associated with considerable morbidity. Aim To investigate potential biological mechanisms underlying MCS in a case-control study. Methods Two hundred and twenty-three MCS cases and 194 controls (urban females, aged 30-64 years) fulfilled reproducible eligibility criteria with discriminant validity. Routine laboratory results and serum levels of volatile organic compounds (VOCs) were compared.

  3. Lymphoma incidence, survival and prevalence 2004–2014: sub-type analyses from the UK's Haematological Malignancy Research Network

    PubMed Central

    Smith, A; Crouch, S; Lax, S; Li, J; Painter, D; Howell, D; Patmore, R; Jack, A; Roman, E

    2015-01-01

    Background: Population-based information about cancer occurrence and survival are required to inform clinical practice and research; but for most lymphomas data are lacking. Methods: Set within a socio-demographically representative UK population of nearly 4 million, lymphoma data (N=5796) are from an established patient cohort. Results: Incidence, survival (overall and relative) and prevalence estimates for >20 subtypes are presented. With few exceptions, males tended to be diagnosed at younger ages and have significantly (P<0.05) higher incidence rates. Differences were greatest at younger ages: the <15 year male/female rate ratio for all subtypes combined being 2.2 (95% CI 1.3–3.4). These gender differences impacted on prevalence; most subtype estimates being significantly (P<0.05) higher in males than females. Outcome varied widely by subtype; survival of patients with nodular lymphocyte predominant Hodgkin lymphoma approached that of the general population, whereas less than a third of those with other B-cell (e.g., mantle cell) or T-cell (e.g., peripheral-T) lymphomas survived for ?5 years. No males/female survival differences were detected. Conclusions: Major strengths of our study include completeness of ascertainment, world-class diagnostics and generalisability. The marked variations demonstrated confirm the requirement for ‘real-world' data to inform aetiological hypotheses, health-care planning and the future monitoring of therapeutic changes. PMID:25867256

  4. Myeloablative radioimmunotherapy in conditioning prior to haematological stem cell transplantation: closing the gap between benefit and toxicity?

    PubMed

    Buchmann, Inga; Meyer, Ralf G; Mier, Walter; Haberkorn, Uwe

    2009-03-01

    High-dose radio-/chemotherapy in the context of autologous and allogeneic haematopoietic stem cell transplantation is a double-edged sword. The requirement for dose intensification is linked to an increase in toxicity to noninvolved organs. Particularly for older patients and patients with comorbidities, efficient but toxicity-reduced schemes are needed. Myeloablative radioimmunotherapy is a targeted, internal radiotherapy that uses radiolabelled monoclonal antibodies (mAb) with affinity to the bone marrow. It involves the administration of high radiation doses (up to 30 Gy) to the bone marrow and spleen but without exposing radiosensitive organs to doses higher than 1-7 Gy. Added to conventional or intensity-reduced conditioning, myeloablative radioimmunotherapy may achieve a pronounced antileukaemic effect with tolerable toxicities. A rational and individual design of the ideal nuclide-antibody combination optimizes therapy. The anti-CD33, anti-CD45 and anti-CD66 mAbs appear to be ideal tracers so far. The beta-emitter (90)Y is coupled by DTPA and is the best nuclide for myeloablation. Approval trials for DTPA anti-CD66 mAb are underway in Europe, and in the near future these therapies may become applicable in practice. This review gives an overview of current myeloablative conditioning radioimmunotherapy. We discuss the selection of the optimal radioimmunoconjugate and discuss how radioimmunotherapy might be optimized in the future by individualization of therapy protocols. We also highlight the potential advantages of combination therapies. PMID:19130053

  5. Haematological and biochemical changes in Fasciola gigantica infected Buffaloes fed on diet containing deoiled mahua (Bassia latifolia) seed cake

    Microsoft Academic Search

    P. Singh; A. K. Verma; Ani Bency Jacob; S. C. Gupta; U. R. Mehra

    2011-01-01

    A study was conducted to evaluate the effect of condensed tannins and saponins from deoiled mahua seed cake (DMSC) on the development of fasciolosis. Fifteen male buffaloes were randomly divided into 3 groups of five each and were orally infected with F. gigantica metacercaria @ 800\\/animal. DMSC was included in the concentrate mixture of group 2 (M5) and 3 animals

  6. The validation and application of the technicon H * 1 for the complete automated evaluation of laboratory animal haematology

    Microsoft Academic Search

    D. T. Davies; G. V. Fisher

    1991-01-01

    The Technicon H*1 has been used in human medicine for many years. Recently, Technicon have modified the instrument's software to allow the evaluation of rodent and dog blood samples. This article presents the results of the validation studies we performed with the Technicon H*1 for use with animal blood and describes aspects of precision, accuracy, sample storage and the ability

  7. Comparative Haematological Screening of Urban and Rural Pregnant Women Attending Antenatal Clinics in Lagos and Its Environs.

    ERIC Educational Resources Information Center

    Abidoye, R. O.; Olukoya, A. A.

    1993-01-01

    Compared blood screening data for 200 urban and rural pregnant women in Nigeria. Found that rural subjects had a greater incidence of moderate anemia than did urban subjects, and corpuscular hemoglobin concentrations fell with increased gestational age. No relationship was found between hemoglobin counts and nutrition habits. (HTH)

  8. Clinico-pathological profile of acute promyelocytic leukaemia at Al-Amal Oncology-Haematology Centre, Qatar.

    PubMed

    Ibrahim, F A; Yassin, M A; El-Ayoubi, H R; Alhiji, I A; Albinali, A S; Almansour, S M; Qafoud, F M

    2010-09-01

    This cases series describes the profile of adult patients with acute promyelocytic leukaemia (APt) at a referral hospital in Qatar. Of 34 acute myeloid leukaemia (AML) cases diagnosed, 11(32%) were classified as APt. Disseminated intravascular coagulation was common at presentation (91%). Severe thrombocytopenia was seen in 73%, leukocytosis in 55% and severe anaemia in 45%. Only 2 patients were of the classic hypergranular type. In the remaining 9 patients, 3 morphological subtypes were recognized: microgranular variant (6 patients), hyperbasophilic (2 patients) and regular nuclear outline M3r (1 patient). Translocation t(15;17) was detected in 63% of cases. APL constitutes a high proportion of AML cases in Qatar, with considerable morphological heterogeneity and a oredominance of APL variants with unfavourable oresenting features. PMID:21218723

  9. Comparison of carbonic anhydrase activity and other haematological values of two Eutherian and three Australian marsupial mammals.

    PubMed

    Dodgson, S J; Holland, R A

    1982-01-01

    1. Carbonic anhydrase activity and 2,3-diphosphoglycerate (2,3-DPG) concentration were determined in whole blood from humans (Homo sapiens), rabbits (Oryctolagus cuniculus), eastern grey kangaroos (Macropus giganteus), pademelons (Thylogale billardierii) and brush-tailed possums (Trichosurus vulpecula). 2. Marsupial blood carbonic anhydrase activity increased as species body size decreased. 3. T. billardierii haemoglobin was found to have a polymorphism which may be the same (beta 2 = histidine or glutamine) as that of M. giganteus. 4. The concentration of 2,3-DPG int e red cells of T. billardierii was approximately equal to that of the haemoglobin tetramer. Levels of 2,3-DPG in the other species were similar to those previously reported. PMID:6125313

  10. Antihepatotoxic potential of Trianthema portulacastrum in carbon tetrachloride-induced chronic hepatocellular injury in mice: Reflection in haematological, histological and biochemical characteristics

    Microsoft Academic Search

    Animesh Mandal; Ranajit Karmakar; Subrata Bandyopadhyay; Malay Chatterjee

    1998-01-01

    The effect of an ethanolic extract of the plantTrianthema portulacastrum L. on the CCI4-induced chronic hepatocellular damage of Swiss albino mice has been investigated. The normal mice received olive oil (0.2\\u000a ml\\/mouse) for five weeks. The CCI4 control mice, on the other hand, received CCI4 (0.05 ml\\/mouse) in olive oil for five weeks. The extract was administered at the dose

  11. The spectrum of Fusarium infection in immunocompromised patients with haematological malignancies and in non-immunocompromised patients: a single institution experience over 10 years.

    PubMed

    Musa, M O; Al Eisa, A; Halim, M; Sahovic, E; Gyger, M; Chaudhri, N; Al Mohareb, F; Seth, P; Aslam, M; Aljurf, M

    2000-03-01

    Fusarium is a newly emerging fungal pathogen associated with significant morbidity and mortality in the immunocompromised host. We have reviewed our hospital's experience with Fusarium between 1985 and 1995. Fusarium species were isolated from 22 specimens, representing 11 patients. Cases were not clustered by time period. The median age of the patients was 36.5 years (range 17-69 years). The sources of the organism were 12 skin lesions from eight patients, seven blood cultures from two patients and one specimen each from a Hickman catheter tip, nail clippings and a bronchoalveolar lavage. Seven of the patients had chemotherapy-induced neutropenia when the Fusarium was isolated. Five of them developed invasive fusarosis during acute leukaemia induction treatment. They remained neutropenic, and none survived. The other two patients recovered from neutropenia and were treated successfully for this infection. The remaining four patients were not neutropenic or immunocompromised. Three grew Fusarium from skin or nail clippings and one from bronchial alveolar lavage (BAL). There was no evidence of invasive disease in any of the four. None of them received antifungal therapy, and they were all alive at last follow-up. We conclude that Fusarium is a newly emerging infection in neutropenic patients. A high index of suspicion, especially for skin lesions, will help in early diagnosis before systemic and visceral dissemination. Excision of the initial focus of infection and antifungal therapy, aided by speedy neutrophil recovery, are likely to protect patients threatened with these fatal infections. Fusarium isolated from non-neutropenic, non-immunosuppressed patients is not significant and does not merit systemic antifungal treatment. PMID:10759712

  12. Clinical, haematological and biochemical alterations in heat intolerance (panting) syndrome in Egyptian cattle following natural foot-and-mouth disease (FMD).

    PubMed

    Ghanem, Mohamed M; Abdel-Hamid, Omnia M

    2010-08-01

    Clinical signs of heat intolerance (panting) syndrome were observed in Holstein cows in a private farm in Egypt. There were heat intolerance (fever), panting, profuse salivation, hirsutism, lameness and reduced milk production. Blood and serum samples were collected from ten diseased cows and five apparently healthy cows as control. Serological tests confirmed the presence of non-structural protein of foot-and-mouth disease (FMD) infection. There were significant reductions in the total red blood cell count with increased leucocytic and lymphocytic counts in diseased group compared to control. The serum Na, Cl, Ca, Mg, Zn and Fe were significantly reduced but P was increased in diseased animals compared to control. The total protein, albumin, cholesterol and cortisol were significantly reduced but the glucose and malonaldehyde were significantly increased in diseased cows. This was the first report in Egypt to describe the clinical and haemato-biochemical changes in panting syndrome following FMD. PMID:20229223

  13. Impact of environmental enrichment in mice. 1: Effect of housing conditions on body weight, organ weights and haematology in different strains

    Microsoft Academic Search

    P.-P. Tsai; U. Pachowsky; H. D. Stelzer; H. Hackbarth

    2002-01-01

    Summary Currently, environmental enrichment is a very common means of improving animal well- being, especially for laboratory animals. Although environmental enrichment seems to be a possible way for improving the well-being of animals, the consideration of housing laboratory animals should not only focus solely on animal well-being, manpower and economics but also on the precision and accuracy of the experimental

  14. Ivermectin treatment of free-ranging endangered Australian sea lion (Neophoca cinerea) pups: effect on hookworm and lice infection status, haematological parameters, growth, and survival.

    PubMed

    Marcus, Alan D; Higgins, Damien P; Gray, Rachael

    2015-07-01

    A placebo-controlled study was used to investigate the effectiveness of ivermectin to treat hookworm (Uncinaria sanguinis) and lice (Antarctophthirus microchir) infections in free-ranging Australian sea lion (Neophoca cinerea) pups and to test the hypotheses that these parasitic infections cause anaemia, systemic inflammatory responses, and reduced growth, and contribute towards decreased pup survival. Ivermectin was identified as an effective and safe anthelmintic in this species. Pups administered ivermectin had significantly higher erythrocyte counts and significantly lower eosinophil counts compared to controls at 1-2 months post-treatment, confirming that U. sanguinis and/or A. microchir are causatively associated with disease and demonstrating the positive effect of ivermectin treatment on clinical health parameters. Higher growth rates were not seen in ivermectin-treated pups and, unexpectedly, relatively older pups treated with ivermectin demonstrated significantly reduced growth rates when compared to matched saline-control pups. Differences in survival were not identified between treatment groups; however, this was attributed to the unexpectedly low mortality rate of recruited pups, likely due to the unintended recruitment bias towards pups >1-2 months of age for which mortality due to hookworm infection is less likely. This finding highlights the logistical and practical challenges associated with treating pups of this species shortly after birth at a remote colony. This study informs the assessment of the use of anthelmintics as a tool for the conservation management of free-ranging wildlife and outlines essential steps to further the development of strategies to ensure the effective conservation of the Australian sea lion and its parasitic fauna. PMID:25911163

  15. Hygienic safety of reusable tap water filters (Germlyser®) with an operating time of 4 or 8 weeks in a haematological oncology transplantation unit

    Microsoft Academic Search

    Georg Daeschlein; William H Krüger; Christian Selepko; Markus Rochow; Gottfried Dölken; Axel Kramer

    2007-01-01

    BACKGROUND: Microbial safe tap water is crucial for the safety of immunosuppressed patients. METHODS: To evaluate the suitability of new, reusable point-of-use filters (Germlyser®, Aquafree GmbH, Hamburg, Germany), three variations of a reusable filter with the same filter principle but with different outlets (with and without silver) and inner surface coating of the filter encasements (with and without nano-crystalline silver)

  16. Diagnosis and antimicrobial therapy of lung infiltrates in febrile neutropenic patients: Guidelines of the infectious diseases working party of the German Society of Haematology and Oncology.

    PubMed

    Maschmeyer, Georg; Beinert, Thomas; Buchheidt, Dieter; Cornely, Oliver A; Einsele, Hermann; Heinz, Werner; Heussel, Claus Peter; Kahl, Christoph; Kiehl, Michael; Lorenz, Joachim; Hof, Herbert; Mattiuzzi, Gloria

    2009-09-01

    Patients with neutropenia lasting for more than 10d, who develop fever and pulmonary infiltrates, are at risk of treatment failure under conventional broad-spectrum antibacterial therapy. Filamentous fungi are predominant causes of failure, however, multi-resistant gram-negative rods such as Pseudomonas aeruginosa or Stenotrophomonas maltophilia may be involved. Prompt addition of mould-active systemic antifungal therapy, facilitated by early thoracic computed tomography, improves clinical outcome. Non-culture-based diagnostic procedures to detect circulating antigens such as galactomannan or 1,3-beta-d-glucan, or PCR techniques to amplify circulating fungal DNA from blood, bronchoalveolar lavage or tissue specimens, may facilitate the diagnosis of invasive pulmonary aspergillosis. CT-guided bronchoalveolar lavage is useful in order to identify causative microorganisms such as multidrug-resistant bacteria, filamentous fungi or Pneumocystis jiroveci. For pre-emptive antifungal treatment, voriconazole or liposomal amphotericin B is preferred. In patients given broad-spectrum azoles for antifungal prophylaxis, non-azole antifungals or antifungal combinations might become first choice in this setting. Antifungal treatment should be continued for at least 14 d before non-response and treatment modification are considered. Microbial isolates from blood cultures, bronchoalveolar lavage or respiratory secretions must be critically interpreted with respect to their aetiological relevance for pulmonary infiltrates. PMID:19467584

  17. A comprehensive diagnostic approach using galactomannan, targeted ?-d-glucan, baseline computerized tomography and biopsy yields a significant burden of invasive fungal disease in at risk haematology patients.

    PubMed

    Ceesay, M Mansour; Desai, Sujal R; Berry, Lisa; Cleverley, Joanne; Kibbler, Christopher C; Pomplun, Sabine; Nicholson, Andrew G; Douiri, Abdel; Wade, Jim; Smith, Melvyn; Mufti, Ghulam J; Pagliuca, Antonio

    2015-01-01

    Invasive fungal disease (IFD) is difficult to diagnose. We investigated the incidence of IFD and risk factors using the revised European Organization for Research and Treatment of Cancer (EORTC) and the Mycoses Study Group (MSG) definitions. Patients (N = 203) undergoing intensive therapy with expected neutropenia ?10 d were recruited prospectively and followed for a median (range) of 556 (12-730) d. Baseline chest computerized tomography (CT) was performed pre-therapy. Twice-weekly surveillance with galactomannan (GM) was combined with targeted ?-d-glucan (BDG) testing on patients with possible IFD or who were GM-positive. Tissue diagnosis was obtained whenever possible. The cumulative incidence of proven/probable IFD among the 202 evaluable cases after 2 years follow-up was 21%, including 14 proven and 30 probable IFDs. Using either GM or BDG as the sole biomarker (plus host and clinical evidence) the apparent overall incidence of proven/probable IFD was 11% and 16%, respectively. Combined GM/BDG detected all biopsy-proven mould IFD. Baseline CT abnormalities were found in 76/202 (38%) patients. Baseline CT abnormalities and Karnofsky score <90, monocytopenia >10 d and bacteraemia were independent risk factors associated with greater than twofold increased IFD risk. This combined diagnostic approach identified a high incidence of IFD and important risk factors in this cohort. PMID:25179933

  18. Osmotic, ionic and haematological response of the Port Jackson shark Heterodontus portusjacksoni and the common stingaree Trygonoptera testacea upon exposure to diluted seawater

    Microsoft Academic Search

    A. R. Cooper; S. Morris

    1998-01-01

    The osmo-ionoregulatory responses of sub-adult Port Jackson sharks, Heterodontus portusjacksoni (Meyer, 1793), and the stingaree Trygonoptera testacea (Mller and Henle, 1841), were investigated following the transfer from full-strength seawater (100% SW) to either 75 or 50%\\u000a SW for up to 168 h. The plasma osmotic pressure was similarly reduced in both species and remained slightly hyper-osmotic\\u000a to the diluted seawater

  19. Extensive early apoptosis in frozen–thawed CD34-positive stem cells decreases threshold doses for haematological recovery after autologous peripheral blood progenitor cell transplantation

    Microsoft Academic Search

    F. de Boer; AM Dräger; H. M. Pinedo; F. L. Kessler; E. van der Wall; A. R. Jonkhoff; J. van der Lelie; P. C. Huijgens; G. J. Ossenkoppele; G. J. Schuurhuis

    2002-01-01

    Stem cell doses necessary for engraftment after myelo-ablative therapy as defined for fresh transplants vary largely. Loss of CD34+ cell quality after cryopreservation might contribute to this variation. With a new early apoptosis assay including the vital stain Syto16, together with the permeability marker 7-AAD, CD34+ cell viability in leucapheresis samples of 49 lymphoma patients receiving a BEAM regimen was

  20. Allogeneic stem cell transplantation for mantle cell lymphoma--final report from the prospective trials of the East German Study Group Haematology/Oncology (OSHO).

    PubMed

    Krüger, William H; Hirt, Carsten; Basara, Nadezda; Sayer, Herbert G; Behre, Gerhard; Fischer, Thomas; Grobe, Norbert; Maschmeyer, Georg; Niederwieser, Dietger; Dölken, Gottfried

    2014-09-01

    This study was conducted in order to evaluate allogeneic stem cell transplantation (alloSCT) as consolidation for patients with mantle cell lymphoma (MCL). Patients with MCL were included into two prospective trials OSHO #060 (refractory/relapsed) and #074 (de novo). Induction was rituximab and chemotherapy. Responding patients proceeded to alloSCT. Minimal residual disease was monitored by quantitative RT-PCR detecting either t(11;14) or clonospecific CDR-III regions. In case of circulating lymphoma cells, immunomodulation (cyclosporine A withdrawal, rituximab, donor lymphocyte infusion) was initiated. Thirty-three of 39 patients underwent alloSCT after myeloablative (n?=?7) or toxicity-reduced (n?=?26) conditioning. Leukocytes engrafted at day +16 (median, range 0-101) and platelets at day +14 (0-142). Acute graft-versus-host disease stages I-II occurred in 42 % and stages III-IV in 15 %. Five patients have relapsed after SCT. The overall mortality after SCT was 24 % (n?=?8). Median follow-up after SCT was 2.8 years (range 0.0-10.9). Five-year progression-free survival was 67 %, and overall survival 73 % after SCT. The results were comparable for primary MCL and refractory/relapsed disease as well as for related vs. unrelated SCT. Younger patients had a significantly better outcome than the elderly. AlloSCT is a feasible and promising consolidation therapy for relapsed and refractory disease and an attractive option for young patients with de novo MCL of high risk. PMID:24782119

  1. Effect of 12-week vanadate and magnesium co-administration on chosen haematological parameters as well as on some indices of iron and copper metabolism and biomarkers of oxidative stress in rats.

    PubMed

    Scibior, Agnieszka; Adamczyk, Agnieszka; Go??biowska, Dorota; Nied?wiecka, Irmina

    2012-09-01

    Changes in some blood parameters after 12-week administration of sodium metavanadate (SMV; 0.125mgV/ml) or/and magnesium sulphate (MS; 0.06mgMg/ml) in drinking water were studied in outbred male Wistar rats (16 rats/each group) to explore the probable mechanism(s) underlying SMV toxicity and check whether Mg at the level selected during SMV co-administration can protect, at least in part, from a possible deleterious action of SMV. Exposure to SMV alone and in combination with MS (a) led to a decrease in fluid and food intake and body weight gain; (b) predisposed the animals to the development of microcytic-hypochromic anaemia (with excessive liver and spleen Fe deposition, unaltered plasma Fe level and enhanced Zn concentration in the erythrocytes (RBCs) characterized by a reduced haematocrit (Ht) index and haemoglobin (Hb) level, unchanged erythrocyte and reticulocyte count, anisocytosis, lowered total iron binding capacity (TIBC) and elevated transferrin saturation (TS); (c) disturbed Cu homeostasis, but (d) did not influence the leukocyte count and the plasma total antioxidant status (TAS). We suggest that abnormal metabolism and accumulation of Fe as well as an altered Cu status and the RBC Zn level might lead to defective Fe utilization and be a factor promoting the development of Fe-utilization anaemia. The disturbances in the antioxidative capacity reported previously in rats' RBCs after SMV intoxication (?cibior, Zaporowska, Environ. Toxicol. Pharmacol. 30 (2010) 153-161) may suggest that oxidative stress (OS) could also be, in part, involved in the mechanism responsible for the development of anaemia. The Mg dose ingested in combination with V under SMV-MS co-administration (a) was able to decrease, to some extent, the V concentration in the blood, (b) normalized the RBC Mg and Fe levels and (c) restored the values of some parameters of the Fe status near the control values. These results allow a supposition that a higher Mg dose consumed during SMV exposure could have better protective potential and be more effective in limiting the SMV toxicity observed. PMID:22561110

  2. A three-arm phase III randomised trial assessing, in patients with extensive-disease small-cell lung cancer, accelerated chemotherapy with support of haematological growth factor or oral antibiotics

    Microsoft Academic Search

    J P Sculier; M Paesmans; J Lecomte; O Van Cutsem; J J Lafitte; T Berghmans; G Koumakis; M C Florin; J Thiriaux; J Michel; V Giner; M C Berchier; P Mommen; V Ninane; J Klastersky

    2001-01-01

    The European Lung Cancer Working Party (ELCWP) designed a 3-arm phase III randomised trial to determine the role of accelerated chemotherapy in extensive-disease (ED) small-cell lung cancer (SCLC). Eligible patients were randomised between the 3 following arms: (A) Standard chemotherapy with 6 courses of EVI (epirubicin 60 mg m–2, vindesine 3 mg m–2, ifosfamide 5 g m–2; all drugs given

  3. Fatal haemoptysis in pulmonary filamentous mycosis: an underevaluated cause of death in patients with acute leukaemia in haematological complete remission. A retrospective study and review of the literature. Gimema Infection Program (Gruppo Italiano Malattie Ematologiche dell'Adulto)

    PubMed

    Pagano, L; Ricci, P; Nosari, A; Tonso, A; Buelli, M; Montillo, M; Cudillo, L; Cenacchi, A; Savignana, C; Melillo, L

    1995-03-01

    A retrospective study on a consecutive series of 116 patients affected by acute leukaemia with documented pulmonary filamentous mycosis (FM) admitted between 1987 and 1992 to 14 tertiary-care hospitals in Italy was made in order to evaluate the characteristics of those patients who developed fatal massive haemoptysis. In 59/116 cases of pulmonary FM the infection was the principal cause of death and in 12 of these patients a massive haemoptysis was responsible for death. The diagnosis of FM infection was made ante-mortem in only four out of these 12 patients. The autopsy was performed in 11/12 patients and documented a FM infection. The mycetes isolated were: Hyphomycetes spp. (three patients), Mucorales spp. (two patients), Aspergillus spp. (seven patients). At the time of the massive haemoptysis the mean neutrophil count was 7.2 x 10(9)/l, and no patient had relevant thrombocytopenia (mean 184 x 10(9)/l, range 28-350) or coagulative abnormalities. The mean time which elapsed between resolution of chemotherapy-induced neutropenia (WBC < 10(9)/l) and occurrence of haemoptysis was 7 d. No signs or symptoms predictive of this fatal complication were identified. Massive haemoptysis can be the cause of death in patients with acute leukaemia and pulmonary FM which in the majority of patients was not diagnosed in vivo. This complication occurs most frequently shortly after the recovery from chemotherapy-induced aplasia. The mechanism of lesion is unknown, but it may involve the vascular tropism of FM and the release of leucocyte enzymes. Better preventive and therapeutic antifungal treatments are needed to avoid this serious, albeit rare, complication. PMID:7734347

  4. Compliance with home-based fortification strategies for delivery of iron and zinc: its effect on haematological and growth markers among 6-24 months old children in north India.

    PubMed

    Sazawal, Sunil; Dhingra, Pratibha; Dhingra, Usha; Gupta, Shilpi; Iyengar, Venkatesh; Menon, Venugopal P; Sarkar, Archana; Black, Robert E

    2014-06-01

    Compliance is a key component in successful implementation of the delivery of micronutrients among children. The present study evaluates the compliance with two home-based food fortification strategies (fortified complementary food or sprinkle) for providing iron and zinc among children aged 6-24 months. A total of 292 children were randomly allocated to receive either rice-based fortified complementary food and nutrition education (Cf = 101), sprinkle and nutrition education (Mp = 97), or nutrition education alone as control (Ed = 94). All the enrolled children were breastfed at the beginning of the study and were advised to continue breastfeeding. Biweekly information on compliance and anthropometry was collected. Complete haemogram estimation was conducted at baseline and end of the study. Compliance with the fortified complementary food was higher compared to sprinkle (Cf = 81%, Mp = 64% child-days). Consumption of the fortified complementary food for 6 months resulted in a significant increase in mean haemoglobin in the intervention group compared to control group (Cf 1.29 +/- 1.6 g/dL; Ed 0.23 +/- 1.3 g/dL; p < 0.001). Our results showed that fortified complementary food had higher compliance than sprinkle and is a suitable delivery mechanism for iron and zinc in preschool children. PMID:25076659

  5. An initial investigation into the effects of isolation and enrichment on the welfare of labratory pigs housed in the PigTurn® system, assessed using tear staining, behaviour, physiology and haematology

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The pig is an increasingly important laboratory animal species. However, a laboratory often requires individual, sterile housing, which may impose stress. Our objective was to determine the effects of isolation and enrichment in pigs housed for 7 days within the PigTurn™ - a novel penning system wit...

  6. Somatic mutation screening using archival formalin-fixed paraffin-embedded tissues by Fluidigm multiplex PCR and Illumina sequencing

    E-print Network

    Wang, Ming; Escudero-Ibarz, Leire; Moody, Sarah; Zeng, Naiyan; Clipson, Alexandra; Huang, Yuanxue; Xue, Xuemin; Grigoropoulos, Nicholas F.; Barrans, Sharon; Worrillow, Lisa; Forshew, Tim; Su, Jing; Firth, Andrew; Martin, Howard; Jack, Andrew; Brugger, Kim; Du, Ming-Qing

    2015-01-01

    of Cambridge, Cambridge, UK; 2Haematological Malignancy Diagnostic Service, St. James’s Institute of Oncology, Leeds, UK; 3Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK; 4Division of Virology, Department of Pathology...

  7. Influence of space flight conditions on phenotypes and functionality of nephritic immune cells of fish (Xiphophorus helleri)

    Microsoft Academic Search

    K. Piepenbreier; J. Renn; R. Fischer; R. Goerlich

    2004-01-01

    Microgravity is considered to directly perturb a number of immunological and haematological parameters in mammalians, and therefore is of fundamental importance in space biology. The viviparous teleost Xiphophorus helleri (swordtail) was used as a \\

  8. IAN F. TANNOCK nitial or acquired resistance to anticancer

    E-print Network

    Cai, Long

    -blood-cell count stimulates the production of haematological growth factors, whichsignalslowlyproliferatingbone succes- sive courses of treatment can lead to tumour remission then regrowth, as is observed in patients

  9. Comeallyescientists,busy andexhausted.Ocomeye,

    E-print Network

    Graur, Dan

    , the thermodynamics of hell, the aerodynamics of angel wings, and the physiology and haematology of resurrection duplication in bacterial evolution, but were engaged in a heated argument on the virtues of old-Earth versus

  10. Am. J.Trop. Med. Hyg., 80(1), 2009, pp. 2427 Copyright 2009 by The American Society of Tropical Medicine and Hygiene

    E-print Network

    Paris-Sud XI, Université de

    suffering from haematologic malignancies (HM) (8 chronic myeloid leukaemia, 8 myeloma, 7 acute lymphoid leukaemia, 6 chronic lymphoid leukaemia, and 6 Hodgkin disease). Fifty-four patients were diarrheic

  11. ANALYSIS OF THE VOLUME OF RED BLOOD CELLS: APPLICATION OF THE EXPECTATION-MAXIMIZATION ALGORITHM TO GROUPED DATA FROM THE DOUBLY-TRUNCATED LOGNORMAL DISTRIBUTION

    EPA Science Inventory

    In accordance with general principles recommended by the International Committee for Standardization in Haematology (1982, Journal of Clinical Pathology 35, 1320-1322), the authors have developed statistical methods for the analysis of red cell volume distributions. To select an ...

  12. Heart failure: not a single organ disease but a multisystem syndrome.

    PubMed

    Warriner, David; Sheridan, Paul; Lawford, Patricia

    2015-06-01

    Heart failure is not simply a single organ disease; rather it is a complex multi-system clinical syndrome, with impairment of endocrine, haematological, musculoskeletal, renal, respiratory and vascular systems, which influence morbidity and mortality. PMID:26053903

  13. Acquired Amegakaryocytic Thrombocytopenic Purpura Associated with Immunoglobulin Deficiency

    Microsoft Academic Search

    Montserrat Rovira; Evarist Feliu; Lourdes Florensa; Soledad Woessner; Dolors Tassies; Emili Montserrat; Joan Llouis Vives-Corrons; Ciril Rozman

    1991-01-01

    Acquired amegakaryocytic thrombocytopenic purpura (AATP) is a haematological disorder characterized by severe thrombocytopenia due to an immunologically induced absence of megakaryocytes in an otherwise normal-appearing bone marrow. A 57-year-old male with a 6-month history of rectal and cutaneous bleeding is reported. Platelet count was 10 × 109\\/l, while other haematological values were within the normal range, except for the presence

  14. Infectivity of promastigotes and amastigotes of Leishmania infantum in a canine model for leishmaniosis

    Microsoft Academic Search

    L Campino; G Santos-Gomes; M. J Riça Capela; S Cortes; P Abranches

    2000-01-01

    Seven dogs experimentally infected with amastigotes or culture promastigotes of Leishmaniainfantum MON-1 were observed for a period of up to 38 months. The course of infection was monitored by clinical and parasitological examinations, haematological and serum protein analysis, and by anti-leishmania antibody levels. Two of the three amastigote-inoculated dogs developed a symptomatic infection with haematological and protein alterations, and a

  15. Persistence of neurological damage induced by dietary vitamin B12 deficiency in infancy

    Microsoft Academic Search

    Ursula von Schenck; Christine Bender-Götze; Berthold Koletzko

    1997-01-01

    A case is reported of a 14 month old boy with severe dietary vitamin B-12 deficiency caused by his mother’s vegan diet. Cinical, electroencephalography (EEG), and haematological findings are described. Cranial magnetic resonance imaging (MRI) showed severe frontal and frontoparietal cranial atrophy. Vitamin B-12 supplements led to a rapid improvement of haematological and neurological symptoms. Serum vitamin B-12 and urinary

  16. Altitude exposure in sports: the Athlete Biological Passport standpoint.

    PubMed

    Sanchis-Gomar, Fabian; Pareja-Galeano, Helios; Brioche, Thomas; Martinez-Bello, Vladimir; Lippi, Giuseppe

    2014-03-01

    The Athlete Biological Passport (ABP) is principally founded on monitoring an athlete's biological variables over time, to identify abnormal biases on a longitudinal basis. Several factors are known to influence the results of these markers. However, the manner in which the altitude factor is taken into account still needs to be standardized. Causal relationships between haematological variables should be correctly integrated into ABP software. In particular, modifications of haematological parameters during and after exposure to different altitudes/hypoxic protocols need to be properly included within detection models. PMID:24115763

  17. Prolonged remission in a child with chronic myeloid leukemia following Parvo virus B19 (B19V) infection.

    PubMed

    Kumar, A; Moulik, N Roy; Kishore, J; Kumar, A; Jain, A

    2015-01-01

    Parvovirus B19 (B19V) has been associated with a wide spectrum of clinico-pathological disorders in human beings depending upon the host immunity. The present report describes a child with chronic myeloid leukemia ( CML) on hydroxyurea in haematological remission, who developed profound erythroid suppression following B19V infection requiring multiple transfusions and withdrawal of hydroxyurea. Despite being off-therapy the child remained in complete clinical and haematological remission till anti B19V antibodies appeared. This case illustrates the ability of B19V infection in suppressing neoplastic myeloid clone, a phenomenon not described earlier. PMID:26068352

  18. Phase I trial and pharmacological study of a 3-hour paclitaxel infusion in children with refractory solid tumours: a SFOP study

    PubMed Central

    Doz, F; Gentet, J C; Pein, F; Frappaz, D; Chastagner, P; Moretti, S; Vassal, G; Arditti, J; Tellingen, O Van; Iliadis, A; Catalin, J

    2001-01-01

    The maximum tolerated dose of paclitaxel administered by 24-hour continuous infusion in children is known. Short infusion might offer equivalent antitumour efficacy and reduced haematological toxicity, without increasing the allergic risk. Our aims were to determine the maximum tolerated dose and the pharmacokinetics of paclitaxel in children when administered in 3-h infusion every 3 weeks. Patients older than 6 months, younger than 20 years with refractory malignant solid tumours were eligible when they satisfied standard haematological, renal, hepatic and cardiologic inclusion criteria with life expectancy exceeding 8 weeks. Paclitaxel was administered as a 3-hour infusion after premedication (dexamethasone, dexchlorpheniramine). Pharmacokinetic analysis and solvent assays (ethanol, cremophor) were performed during the first course. 20 courses were studied in 17 patients; 4 dosage levels were investigated (240 to 420?mg/m2). No dose-limiting haematological toxicity was observed. Severe acute neurological and allergic toxicity was encountered. One treatment-related death occurred just after the infusion at the highest dosage. Delayed peripheral neurotoxicity and moderate allergic reactions were also encountered. Pharmacokinetic analysis showed dose-dependent clearance of paclitaxel and elevated blood ethanol and Cremophor EL levels. Although no limiting haematological toxicity was reached, we do not recommend this paclitaxel schedule in children because of its acute neurological toxicity. © 2001 Cancer Research Campaign http://www.bjcancer.com PMID:11237379

  19. Non-epithelial malignancies and metastatic tumours of the breast

    Microsoft Academic Search

    Mark E O'Donnell; Mark McCavert; Jim Carson; Fred J Mullan; Michael W Whiteside; W Ian

    Introduction: Non-epithelial breast malignancies include primary lymphomas, sarcomas, haematological malignancies, melanomas as well as secondary metastases to the breast. They account for less than 1% of all breast tumours. The demographics and clinical features are similar to epithelial breast cancers but the prognosis and management options are often very different. Most reported series are small with limited follow-up. The main

  20. Somatic mitochondrial DNA mutations in adult-onset leukaemia

    Microsoft Academic Search

    L He; L Luo; S J Proctor; P G Middleton; E L Blakely; R W Taylor; D M Turnbull

    2003-01-01

    Mitochondrial genome instability has recently been demonstrated in a wide variety of human tumours and is implicated in the development of the myelodysplastic syndromes, a heterogeneous group of haematological disorders with an increased risk of malignant transformation. We therefore investigated the incidence of somatic mitochondrial DNA (mtDNA) mutations in patients with adult-onset leukaemia. We sequenced the entire mitochondrial genome from

  1. Severe encephalopathy with epilepsy in an infant caused by subclinical maternal pernicious anaemia: case report and review of the literature

    Microsoft Academic Search

    G. Christoph Korenke; Donald H. Hunneman; Stefan Eber; Folker Hanefeld

    2004-01-01

    Vitamin B 12 deficiency is one of the major causes of megaloblastic anaemia with or without neurological symptoms. We report on a patient manifesting acute encephalopathy, epilepsy, microcephaly and megaloblastic anaemia at the age of 4 months. Vitamin B 12 deficiency in the patient was due to subclinical pernicious anaemia of the mother who exhibited neither haematological nor neurological symptoms.

  2. Serum microRNA screening for DICER1-associated pleuropulmonary blastoma

    E-print Network

    Bailey, Shivani; Raby, Katie L.; Coleman, Nicholas; Murray, Matthew J.

    2014-08-31

    for pediatric oncology patients. Pediatrics 116 2010:126(4):e851-858. 117 118 ... Haematology and Oncology, Addenbrooke’s Hospital, Hills 6 Road, Cambridge, CB2 0QQ, UK 7 2 Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, 8 CB2 1QP, UK 9 3 Department of Histopathology, Addenbrooke’s Hospital, Hills Road...

  3. The ferret, Mustela putorius furo , as a new species in toxicology

    Microsoft Academic Search

    P. C. Thornton; Pauline A. Wright; P. J. Sacra; T. E. W. GOODIERt

    1979-01-01

    Summary Comprehensive studies on the haematology, urine chemistry, serum chemistry and gross pathology have been carried out and reported. Seasonal weight changes were confirmed and were initiated, together with sexual activity, when ferrets were brought in to a constant temperature (18-19°C) and 14-hour daylight cycle during the winter months. Levels of haemoglobin, erythrocytes and haematocrit were higher than in most

  4. Treatment of fungal infections in hematology and oncology

    Microsoft Academic Search

    Angelika Böhme; Markus Ruhnke; Dieter Buchheidt; Meinolf Karthaus; Hermann Einsele; Stefan Guth; Gudrun Heussel; Claus-Peter Heussel; Christian Junghanss; Winfried K. Kern; Thomas Kubin; Georg Maschmeyer; Orhan Sezer; Gerda Silling; Thomas Südhoff; Andrew J. Ullmann

    2003-01-01

    The Infectious Diseases Working Party of the German Society of Haematology and Oncology presents their guidelines for the treatment of fungal infections in patients with hematological and oncological malignancies. These guidelines are evidence-based, considering study results, case reports and expert opinions, using the evidence criteria of the Infectious Diseases Society of America (IDSA). The recommendations for major fungal complications in

  5. Article original Infection exprimentale du canard mulard

    E-print Network

    Paris-Sud XI, Université de

    a été réalisée sur des canards mulards femelles (Cairina mos- chata x Anas platyrhynchos) recevant haematological and biochemical parameters. Eleven-day-old female Mule ducks (Cairina moschata x Anas platyrhynchos) were individually infected per os with a single inoculation contain- * Correspondance et tirés ŕ

  6. Hair Zinc Level in Down Syndrome

    ERIC Educational Resources Information Center

    Yenigun, Ayse; Ozkinay, Ferda; Cogulu, Ozgur; Coker, Canan; Cetiner, Nurten; Ozden, Gonca; Aksu, Oguz; Ozkinay, Cihangir

    2004-01-01

    Immunological, endocrinological, and haematological abnormalities are relatively common in people with Down syndrome (Cuadrado & Barrena, 1996; Decoq & Vincker, 1995; Hestnes et al., 1991; Sustrova & Strbak, 1994; Nespoli, Burgio, Ugazio & Maccario, 1993; Kempski, Chessells & Reeves, 1997; Kivivuori, Rajantie, & Siimes, 1996; David et al., 1996;…

  7. Experimental Mycotoxic Nephropathy in Pigs Provoked by a Diet Containing Ochratoxin A and Penicillic Acid

    Microsoft Academic Search

    S. D. Stoev; S. Vitanov; G. Anguelov; T. Petkova-Bocharova; E. E. Creppy

    2001-01-01

    Mycotoxic nephropathy was induced in 18 young pigs by diets contaminated with strains of Aspergillus ochraceus containing ochratoxin A (OTA) and penicillic acid (PA) at levels corresponding to those naturally encountered in animal feeds in Bulgaria. Haematological and biochemical parameters, as well as the morphological and ultrastructural changes in various internal organs, and especially in the kidneys, were examined at

  8. Investigations on the aetiology of pinching off syndrome in four white-tailed sea eagles (Haliaeetus albicilla) from Germany

    Microsoft Academic Search

    Kerstin Müller; Elvira Schettler; Helga Gerlach; Leo Brunnberg; Hafez Mohamed Hafez; Kim Hattermann; Reimar Johne; Rainer Kollmann; Oliver Krone; Michael Lierz; Sonja Linke; Dörte Lueschow; Annette Mankertz; Hermann Müller; Christina Prusas; Rüdiger Raue; Dirk Soike; Stephanie Speck; Petra Wolf; Kai Frölich

    2007-01-01

    The purpose of this study was to investigate the aetiology of the pinching off syndrome (POS), a generalized feather abnormality affecting free-living nestling of the white-tailed sea eagle (Haliaeetus albicilla) in Europe. For the first time, extensive clinical, haematological, biochemical, virological, bacteriological, nutritional, histopathological, parasitological and electron microscopical examinations were performed on three females and one male suffering from POS.

  9. Automated quantitation of peripheral blood neutrophil activation in patients with myocardial ischaemia

    Microsoft Academic Search

    M. J Leckie; A. H Gomma; I. F Purcell; B Nyawo; A Dewar; D Okrongly; J. F Burman; J Hooper; P. J Barnes; J. R Clague; Trevor T Hansel

    2004-01-01

    Background: Coronary ischaemic syndromes are associated with neutrophil activation. The Bayer automated haematology analysers can detect increased light scatter of neutrophil populations, which correlates with neutrophil activation. We aimed to assess the role of an automated analyser in detecting systemic neutrophil activation in peripheral blood samples of patients with coronary ischaemia. Methods: A prospective cross-sectional study was undertaken in 18

  10. Case Report. Prevention of Clozapine-Induced Granulocytopenia/Agranulocytosis with Granulocyte-Colony Stimulating Factor (G-CSF) in an Intellectually Disabled Patient with Schizophrenia

    ERIC Educational Resources Information Center

    Rajagopal, G.; Graham, J. G.; Haut, F. F. A.

    2007-01-01

    Background: While clozapine is an effective treatment for refractory schizophrenia, its use is limited by haematological side effects. Treatment options that allow continued prescription of clozapine by tackling these side effects will greatly aid patients for whom this medication is all too often their only hope of recovery. Method: In this case…

  11. Leptospirosis: a globally increasing zoonotic disease

    Microsoft Academic Search

    Clare Rock; Deirdre Brady; Patrick Forde; Patricia Lucey; Mary Horgan

    2010-01-01

    A 27-year-old previously healthy man was admitted to the intensive care unit with severe jaundice, dyspnoea with haemoptysis, anaemia, thrombocytopenia and acute renal injury. He had no recent history of foreign travel but had been building a shed in his back garden in Cork, Ireland, for the preceding week. The patient's history, clinical observations, haematological and radiological results were all

  12. A novel galactosyl-binding lectin from the plasma of the blood clam, Anadara granosa (L) and a study of its combining site

    Microsoft Academic Search

    Tarun K. Dam; Manju Sarkar; Jharna Ghosal; Amalesh Choudhury

    1992-01-01

    The marine blood clam species Anadara granosa (L) belong to arcidae, a family with some extraordinary haematological features. The plasma of this species exhibited strong haemagglutinating activities, from which a galactosyl binding lectin, Anadarin P, was purified in a single step affinity chromatography using Sepharose 4B-asialofetuin as an affinity matrix. The purified lectin, eluted with lactose, was found to be

  13. MICROANGIOPATHIC HAEMOLYTIC ANAEMIA AND SYSTEMIC VASCULITIS

    Microsoft Academic Search

    C. N. ROSS; H. REUTER; D. SCOTT; D. V. HAMILTON

    1996-01-01

    SUMMARY Two cases of systemic vasculitis complicated by microangiopathic haemolytic anaemia (MAHA) are described: this association has not previously been reported. Both patients had atypical presentations of their primary disease, one with parotitis and one with a Guillain-Barri syndrome. Other causes of MAHA were excluded and a possible link with macromolecular von Willebrands factor is speculated upon. HAEMATOLOGICAL abnormalities in

  14. Faculty of Health and Medical Sciences School of Health & Social Care

    E-print Network

    Doran, Simon J.

    and Immunology Epidemiology of cancer Cellular, molecular and genetics of cancer Cancer metastasis Tumour in research and practice Site specific cancer: haematological, lung, and GI cancer Pre-requisites of StudyFaculty of Health and Medical Sciences School of Health & Social Care The Science of Cancer Module

  15. Hair zinc level in Down syndrome

    Microsoft Academic Search

    Ayse Yenigun; Ferda Ozkinay; Ozgur Cogulu; Canan Coker; Nurten Cetiner; Gonca Ozden; Oguz Aksu; Cihangir Ozkinay

    2004-01-01

    Immunological, endocrinological, and haematological abnormalities are relatively common in people with Down syndrome (Cuadrado & Barrena, 1996; Decoq & Vincker, 1995; Hestnes et al., 1991; Sustrova & Strbak, 1994; Nespoli, Burgio, Ugazio & Maccario, 1993; Kempski, Chessells & Reeves, 1997; Kivivuori, Rajantie, & Siimes, 1996; David et al., 1996; Gjertson, Sturm & Berger, 1999). Zinc is one of the elements

  16. West Syndrome in an Infant with Vitamin B[subscript 12] Deficiency in the Absence of Macrocytic Anaemia

    ERIC Educational Resources Information Center

    Erol, Ilknur; Alehan, Fusun; Gumus, Ayten

    2007-01-01

    Vitamin B[subscript 12] deficiency in infants often produces haematological and neurological deficits, including macrocytic anaemia, neurodevelopmental delay or regression, irritability, weakness, hypotonia, ataxia, apathy, tremor, and seizures. The diagnosis of vitamin B[subscript 12] deficiency can be difficult when the typical macrocytic…

  17. Dietary copper exposure in the African walking catfish, Clarias gariepinus: Transient osmoregulatory disturbances and oxidative stress

    Microsoft Academic Search

    I. Hoyle; B. J. Shaw; R. D. Handy

    2007-01-01

    There are few dietary Copper (Cu) toxicity studies on warm water fish. We used the African walking catfish (Clarias gariepinus) to perform the first in vivo dietary Cu toxicity study on this species. We measured end points normally associated with metal toxicity (nutritional performance, haematology, histology, tissue Cu, Na+, and K+, Na+K+-ATPase activity) and add to the limited data on

  18. [Veterinary aspects of the raising of mountain goats (Oreamnos americanus)].

    PubMed

    Seidel, B

    1991-03-01

    An empirical report outlines veterinary problems in Rocky Mountain Goats kept in climatic conditions of Central Europe. Described are therapeutic findings in the treatment of infections, parasitosis, disorders of the extremities, disturbances of reproduction, and injuries as well as haematological findings and experiences made during immobilization of Rocky Mountain Goats. PMID:2049057

  19. A Comparison of the Susceptibility of Growing Mukota and Large White Pigs to Infection with Ascaris suum

    Microsoft Academic Search

    J. Zanga; M. Chimonyo; A. Kanengoni; K. Dzama; S. Mukaratirwa

    2003-01-01

    The influence of A. suum infection on the haematology, liver-related serum enzymes, blood urea and live weight gain in Mukota and Large White (LW) weaner pigs was compared. Six pigs of each genotype were infected with a single dose of 4000 A. suum eggs per pig and another six were not. The pigs were kept for 100 days. Blood was

  20. Liminal Biopolitics: Towards a Political Anthropology of the Umbilical Cord and the Placenta

    Microsoft Academic Search

    Pablo Santoro

    2011-01-01

    One of the most intriguing bio-objects in the emerging field of regenerative medicine is umbilical cord blood. Employed in existing haematological therapies, but also loaded with potentialities for future uses, cord blood has been lately the focus of a regulatory debate which confronts public and private forms of biobanking. This article explores the political and anthropological side of this debate,

  1. Automated detection of micro-organisms in blood cultures by means of the Malthus Microbiological Growth Analyser

    Microsoft Academic Search

    D F Brown; M Warner; C E Taylor; R E Warren

    1984-01-01

    A prototype Malthus Microbiological Growth Analyser was compared with conventional methods for examining blood cultures in a trial of 651 cultures mostly from patients with haematological malignancy or undergoing haemodialysis or renal transplantation. Of 100 significantly positive cultures, organisms from 82 grew in the conventional aerobic (+ CO2) bottle, 78 in the conventional anaerobic bottle and 71 in the Malthus

  2. Expression Profile of Genes Representing Varied Spectra of Cell Lineages in Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells

    Microsoft Academic Search

    Young Joon Moon; Myoung Woo Lee; Mal Sook Yang; Sun Kyung Kim; Joon Seong Park; Hugh C. Kim; Hy-Sook Kim; Kwang-Ho Lee; Young-Jin Kim; Jeongeun Choi

    2005-01-01

    Bone marrow (BM) transplantation is a well-established classical treatment for children with malignant haematologic diseases including leukaemia or fatal metabolic diseases [1]. Mononuclear cells (MNCs) in human BM undergo haemopoiesis into distinct cell lineages such as haemopoietic stem cells and mesenchymal stem cells (MSCs), which are capable of differentiating into varied spectra of cell lineages [2, 3]. Human umbilical cord

  3. 363Vet. Res. 35 (2004) 363368 INRA, EDP Sciences, 2004

    E-print Network

    Paris-Sud XI, Université de

    2004-01-01

    . Therefore, 238 dogs admitted to the Small animal clinic in Ljubljana from the years 2000 to 2002 were tested, 14 dogs (5.9%) were determined as being infected with babesiae. Clinical signs relating to acute haemolysis, fever, anorexia, depression and haematological abnormalities such as anaemia and thrombocytopenia

  4. Facteurs pronostiques des leucémies aiguës et des lymphomes

    Microsoft Academic Search

    S de Botton; P Fenaux; B Quesnel

    2002-01-01

    During the last two decades, striking advances in characterization and risk-adapted treatments of haematological malignancies have led to a substantial improvement in long-term survival rates. Thus, patients admitted in intensive care unit, either at diagnosis or during the first line therapy, should be managed as patients that could be potentially cured. The main prognostic factors are detailed in this review.

  5. [Disorders of the larynx and chronic inflammatory diseases].

    PubMed

    Pickhard, A; Smith, E; Rottscholl, R; Brosch, S; Reiter, R

    2012-12-01

    Chronic inflammatory diseases including tuberculosis, rheumatic disorders (rheumatoid arthritis, sarcoidosis, systemic lupus erythematosus, Wegeners's granulomatosis, relapsing polychondritis) and reflux disease are considered as systemic diseases, and may affect the larynx. The clinical symptoms are often unspecific, leading to prolonged intervals to diagnosis. Solid and haematological tumours should be considered in differential diagnosis and may require bioptic sampling. Treatment may require interdisciplinary approach. PMID:23044789

  6. An Audit of Clinic Consultation Times in a Cancer Centre: Implications for National Manpower Planning

    Microsoft Academic Search

    R. J. Benson; N. G. Burnet; M. V. Williams; L. T. Tan

    2001-01-01

    A departmental audit was conducted to assess the frequency, extent and causes of late completion of oncology clinics. Data were collected prospectively from clinical, medical, haematological and multidisciplinary oncology clinics. The data recorded included: clinic start and finish times, number of patients seen, type of consultation, number of doctors in each clinic, time spent by the doctor with the patient,

  7. Development of a new MLST scheme for differentiation of Fusarium solani Species Complex (FSSC) isolates

    Microsoft Academic Search

    Anne Debourgogne; Cécile Gueidan; Christophe Hennequin; Nelly Contet-Audonneau; Sybren de Hoog; Marie Machouart

    2010-01-01

    Fungi belonging to the Fusarium solani Species Complex (FSSC) are well known plant pathogens. In addition to being the causative agent of some superficial infections, FSSC has recently emerged as a group of common opportunistic moulds, mainly in patients with haematological malignancies. Molecular typing methods are essential in order to better understand the epidemiology of such opportunistic agents with the

  8. Changes in non-specific biomarkers in the Mediterranean barbel (Barbus meridionalis) exposed to sewage effluents in a Mediterranean stream (Catalonia, NE Spain).

    PubMed

    Maceda-Veiga, Alberto; Monroy, Mario; Viscor, Ginés; De Sostoa, Adolfo

    2010-11-01

    The effluents from sewage treatment plants strongly influence the water quality and flow of Mediterranean streams. These effluents play a crucial role in maintaining the aquatic communities of these ecosystems, particularly in the absence of natural flow resulting from climate constraints or intensive water use. To detect the ecological effects of these effluents, we used non-lethal biomarkers in Barbus meridionalis and traditional assessment protocols in the Ripoll River (NE of Spain). Our results demonstrate the utility of haematological parameters. In comparison with fish at reference sites, we detected a decrease in haematocrit and haemoglobin, neutrophilia, lymphopenia, monocytosis, a rise in the nucleo-cytoplasmatic ratio of erythrocytes and an increase in the frequency of abnormal, immature and senescent erythrocytes. Many haematological parameters correlated significantly with the environmental parameters measured. In addition to these haematological alterations, histopathological examination also revealed damage in fish livers but no impact was detected by the regional index of biotic integrity using fish as bioindicators. To the best of our knowledge, this study is the first to report this battery of haematological parameters as biomarkers on a freshwater fish in a Mediterranean stream. In a region in which more than 50% of native fish species are classified as endangered or vulnerable, there is an urgent need to find biomarkers that do not harm the animals. PMID:20810176

  9. Gloriosa superba ingestion: Hair loss and acute renal failure

    PubMed Central

    Khanam, P. S.; Sangeetha, B.; Kumar, B. V.; Kiran, U.; Priyadarshini, P. I.; Ram, R.; Sridhar, M. S.; Kumar, V. S.

    2015-01-01

    Gloriosa superba is a plant that grows wild in several parts of South India. Tubers of this plant contain several alkaloids. Acute intoxication following the ingestion of G. superba results in gastrointestinal and haematological abnormalities, hepatic and renal insufficiency, cardiotoxicity and hair loss. We present a case with typical features of G superba toxicity.

  10. Effect of inositol hexaphosphate (IP6) on human normal and leukaemic haematopoietic cells

    Microsoft Academic Search

    Giorgio Lambertenghi Deliliers; Federica Servida; Nicola S. Fracchiolla; Clara Ricci; Chiara Borsotti; Gualtiero Colombo; Davide Soligo

    2002-01-01

    Summary. Inositol hexaphosphate (IP6), a naturally poly- phosphorylated carbohydrate, has been reported to have significant in vivo and in vitro anticancer activity against numerous tumours, such as colon, prostate, breast, liver and rhabdomyosarcomas. To confirm this activity in haematological malignancies and to characterize some of the mechanisms of IP6 action, we analysed its effects on human leukaemic cell lines and

  11. Shwachman's syndrome. A review of 21 cases

    Microsoft Academic Search

    P J Aggett; N P Cavanagh; D J Matthew; J R Pincott; J Sutcliffe; J T Harries

    1980-01-01

    21 patients (10 male, 11 female) aged between 11 months and 29 years with Shwachman's syndrome are reviewed. All patients had exocrine pancreatic insufficiency. Haematological features included neutropenia in 19 (95%), anaemia in 10 (50%), and thrombocytopenia in 14 (70%); one patient developed erythroleukaemia. Severe infections occurred in 17 (85%) from which 3 (15%) died. Only one child exceeded the

  12. This has been a difficult month with too many problems all coming together and rather a lot of sickness just to make life interesting. All the kids and I have spent 5 to 10 days each down

    E-print Network

    Bushman, Frederic

    by the warranty. It means at present we can't do CD4 counts on our children. Then the water-bath broke... By which time I was crying! And then a new machine arrived ­ a Haematology Analyser for one of the research

  13. Dissemination via the lymphatic or angiogenic route impacts the pathology, microenvironment and hypoxia-related drug response of lung metastases.

    PubMed

    Gieling, Roben G; Fitzmaurice, Richard J; Telfer, Brian A; Babur, Muhammad; Williams, Kaye J

    2015-08-01

    Complications associated with the development of lung metastases have a detrimental effect on the overall survival rate of many cancer patients. Preclinical models that mimic the clinical aspects of lung metastases are an important tool in developing new therapy options for these patients. The commonly used intravenous models only recapitulate dissemination of cancer cells to the lungs via the haematological route. Here we compared spontaneous and intravenous lung metastases of the highly metastatic KHT mouse fibrosarcoma cells after injecting KHT cells into the subcutaneous layer of the skin or directly into the tail vein. In contrast to the intravenous model, metastases spontaneously arising from the subcutaneous tumours disseminated most consistent with the lymph nodes/lymphatics route and were more hypoxic than the metastases observed following tail-vein administration and haematological spread. To ascertain whether this impacted on drug response, we tested the effectiveness of the hypoxia-sensitive cytotoxin AQ4N (Banoxantrone) in both models. AQ4N was more effective as an anti-metastatic drug in mice with subcutaneous KHT tumours, significantly reducing the metastatic score. Complementing the KHT studies, pathology studies in additional models of spontaneous lung metastases showed haematological (HCT116 intrasplenic implant) or mixed haematological/lymphatic (B16 intradermal implant) spread. These data suggest that preclinical models can demonstrate differing, clinically relevant dissemination patterns, and that careful selection of preclinical models is required when evaluating new strategies for targeting metastatic disease. PMID:26112891

  14. Theileria ( Babesia ) equi and Babesia caballi Infections in Horses in Galicia, Spain

    Microsoft Academic Search

    A. T. Camacho; F. J. Guitian; E. Pallas; J. J. Gestal; A. S. Olmeda; M. A. Habela; S. R. Telford III; A. Spielman

    2005-01-01

    The control of equine piroplasmosis is becoming increasingly important to maintain the international market open to the horse industry. The purpose of this study was to demonstrate the occurrence of equine piroplasmosis (Theileria equi and Babesia caballi) in Galicia, north-west Spain, and to compare haematological and serum biochemistry parameters between non-parasitaemic horses and horses parasitaemic with T. equi and B.

  15. Effects of azathioprine on response of renal anaemia to subcutaneous recombinant human erythropoietin

    Microsoft Academic Search

    J E Howarth; H M Waters; D Shanks; K Hyde; J A Yin; C G Geary; E Anastassiades; D Howarth; R Gokal

    1993-01-01

    AIMS: To determine the effect of concomitant azathioprine treatment on the response of patients with renal failure to treatment with subcutaneous recombinant human erythropoietin (r-HuEPO). METHODS: Two groups of patients with renal failure not receiving haemodialysis were studied. One comprised seven patients receiving erythropoietin alone, the second consisted of nine patients who were also treated with azathioprine. The haematological changes

  16. Experimental Study on Haematotoxic and Leukaemogenic Effects of Trichlorophone and Dimethoate

    Microsoft Academic Search

    R. Stieglitz; W. Gibel; W. Werner; H. Stobbe

    1974-01-01

    The haematological effects of the economically important alkylphosphates, trichlorophone and dimethoate, were tested experimentally on rats. A pronounced haematotoxic action of these pesticidal organophosphate compounds was found, including severe hyperplasia of the haematopoietic parenchyma of bone marrow and extra-osseous myeloid metaplasia, particularly in liver and spleen. The interpretation of the results in terms of a myeloproliferation (panmyelosis) and myeloid leukosis

  17. Life-threatening complications of transient abnormal myelopoiesis in neonates with Down syndrome

    Microsoft Academic Search

    Sabine Dormann; Marcus Krüger; Roland Hentschel; Regina Rasenack; Brigitte Strahm; Udo Kontny; Charlotte Niemeyer

    2004-01-01

    Neonates with Down syndrome can present with a haematological disorder called transient abnormal myelopoiesis (TAM). While TAM is usually a self-limiting disease, patients with severe complications such as hydrops fetalis, cardiorespiratory failure and liver fibrosis have been described. Here, we present five consecutive neonates with trisomy 21 and TAM, four of whom were critically ill and were therefore treated with

  18. Particulate air pollution and the blood

    Microsoft Academic Search

    Anthony Seaton; Anne Soutar; Vivienne Crawford; Robert Elton; Susan McNerlan; John Cherrie; Monika Watt; Raymond Agius; Robert Stout

    1999-01-01

    BACKGROUNDParticulate air pollution has been associated with excess deaths from, and increases in hospital admissions for, cardiovascular disease among older people. A study was undertaken to determine whether this may be a consequence of alterations in the blood, secondary to pulmonary inflammation caused by the action of fine particles on alveolar cells, by repeatedly measuring haematological factors in older people

  19. Comparison of two formulae for the calculation of glomerular filtration in the dosage of zoledronic acid

    Microsoft Academic Search

    L. Ortega; C. Rodríguez; E. Ramírez Herráiz; N. Garrido; R. Díez Fernández; A. M. Iglesias Bolańos; M. Arteta Jiméneza; G. Segrelles; L. Jiménez; J. Nebot; E. Fernández; M. Longoni; C. Estany; R. Albornoz; R. Arias; M. Arteta Jiménez; Servicio de Farmacia

    2011-01-01

    Objective: To validate the use of a formula that does not require the patient's weight (Levey formula) for calculating creatinine clearance in the adjustment of the dosage of zoledronic acid. Method: Prospective observational study in which zoledronic acid prescriptions in the Oncology and Haematology departments were recorded over the course of 8 months. The adjustment of the dose of zoledronic

  20. Protective effects of fruit extracts of Hippophae rhamnoides L. against arsenic toxicity in Swiss albino mice

    Microsoft Academic Search

    R Gupta; S JS Flora

    2006-01-01

    Seabuckthorn (Hippophae rhamnoides L.) berry has a long history of applications as a food and medicinal ingredient in eastern countries. The present study was carried out to investigate the effect of different fruit extracts of H. rhamnoides on altered biochemical parameters indicative of haematological alterations, tissue oxidative stress, and arsenic concentration in arsenicexposed mice (2.5 mg\\/kg body weight, intraperitoneally). Two

  1. Effects of social isolation and environmental enrichment on laboratory housed pigs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The pig is becoming an increasingly important laboratory animal species. However, a laboratory setting often requires individual and sterile housing, which may impose stress. The objective of this study was to determine physiological, haematological and behavioral effects of isolation and environmen...

  2. Original article Effects of intramuscular injections of folic acid

    E-print Network

    Paris-Sud XI, Université de

    Original article Effects of intramuscular injections of folic acid on serum folates, haematological each, were used to determine the effect of intramuscular injections of folic acid on serum folates containing 5 mg/ml folic acid, while in the other the animals were non-injected controls. The concen- tration

  3. Acute admissions of patients with sickle cell disease who live in Britain

    Microsoft Academic Search

    M Brozovi?; S C Davies; A I Brownell

    1987-01-01

    All acute admissions of patients with sickle cell disease who lived in the London borough of Brent and attended this hospital were analysed for a period of one year. Sixty three of the 211 patients who were followed up by the haematology department required 161 acute admissions during the year. Most admissions (126) were for the 42 patients with homozygous

  4. A phase I\\/II study of gemcitabine and fractionated cisplatin in an outpatient setting using a 21-day schedule in patients with advanced and metastatic bladder cancer

    Microsoft Academic Search

    S A Hussain; D D Stocken; P Riley; D H Palmer; D R Peake; J I Geh; D Spooner; N D James

    2004-01-01

    A randomised phase III trial of MVAC (methotrexate, vincristine, doxorubicin, cisplatin) vs gemcitabine and cisplatin (GC) (G 1000 mg m?2 days 1, 8, and 15 plus C 70 mg m?2 day 2, q 4 wks) indicated GC had similar efficacy and lower toxicity (JCO 2000). Significant haematologic toxicities in the GC arm occurred on day 15, necessitating dose adjustments in

  5. Cyclosporin A Monotherapy in Young Indian Aplastic Anaemia Patients

    Microsoft Academic Search

    Subhash Varma; Neelam Varma; Pankaj Malhotra; Sanjay Singh; Deepika R

    Patients of AA (bone marrow proven) attending haematology clinic of Internal Medicine, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh who were not suitable or unable to afford hone marrow transplantation or who were excluded from antithymocyte globulin therapy because of poor response seen in young age were recruited in the study. Written informed consent was taken before enrollment.

  6. Low concentration effects of endosulfan insecticide on reproductive behaviour in the tropical cichlid fish Sarotherodon mossambicus

    Microsoft Academic Search

    Peter Matthiessen; James W. M. Logan

    1984-01-01

    Endosulfan insecticide is used in the tropics for killing tsetse flies, being applied from aircraft as an aerosol at doses between 6 and 25 g\\/ha\\/application. Dose rates such as these in the Okavango Delta of Botswana have caused an average 1% mortality in shallowwater fish populations (Fox & Matthiessen 1982), and produced short-term pathological and haematological changes in survivors (Matthiessen

  7. number of returns to oestrus was larger with R2, whereas more abortions were observed with Ri, especially during the 3rd and 4th cycles.

    E-print Network

    Boyer, Edmond

    and haematological profiles in reproductive sows influence of the physiological stage and of the reproductive cycle management system (2.5 cycles per sow and per year) and in satisfactory sanitary conditions, blood samplings, urea and cholesterol contents as compared with late lactation. The influence of the reproductive cycle

  8. Can primary immunodeficiencies help to provide insights into infectious risks

    E-print Network

    Cai, Long

    lymphoma and various B cell-dependent autoimmune and inflammatory disorders14 . It causes prolonged B cell and autoimmune diseases and haematological malignancies. However, inhibit- ing the expression or blocking that are involved in various innate and adap- tive immunological processes7,8 . It has been shown that autoimmunity

  9. Ultrastructure of Hepatozoon boigae (Mackerras, 1961) nov. comb. from brown tree snakes, Boiga irregularis , from northern Australia

    Microsoft Academic Search

    K. A. Jakes; P. J. O'Donoghue; J. Whittier

    2003-01-01

    Intraerythrocytic bodies identified as haemogregarine gamonts were found in 29% of 97 brown tree snakes (Boiga irregularis) examined during a haematological survey of reptiles in Australasia during 1994–1998. The morphological characteristics of the parasites were consistent with those of Haemogregarina boigae Mackerras, 1961, although the gamonts were slightly larger and lacked red caps but contained distinctive polar grey capsules. Gamonts did

  10. Distribution of Different PML/RAR? bcr Isoforms in Indian Acute Promyelocytic Leukemia (APL) Patients and Clinicohematological Correlation

    PubMed Central

    Chatterjee, Tathagata; Gupta, Srishti; Sharma, Sanjeevan; Ganguli, Prosenjit

    2014-01-01

    Context Acute promyelocytic leukemia (APL), an AML subtype, is characterized morphologically by abnormal promyelocytes. Molecular studies show three possible bcr isoforms of PML-RAR? fusion gene. This study undertakes analysis of PML-RAR? bcr isoforms and their correlation with haematological parameters and response to treatment in Indian patients. Aims To study different PML-RAR? bcr isoforms in Indian patients and to find any correlation with various haematological parameters and response to treatment Settings and Design Patients diagnosed as APL on morphology or flowcytometry and confirmed by RQ PCR were included in the study. Treated APL patients or patients with relapse and on follow-up were excluded from the study. Methods and Material Twenty patients over thirty one months period were included. The clinical, haematological & morphological features were analysed, the latter using routine & special cytochemical stains on blood and bone marrow. Flow cytometric evaluation using 4-color Beckman Coulter FC 500 and molecular studies using RT PCR Fusion Quant® kits for bcr-1, bcr-2 and bcr-3 of PML-RAR? bcr isoforms on the instrument Rotor Gene™ 3000 were performed. Statistical analysis used Student t test was applied to correlate different bcr isoforms with various haematological parameters and response to treatment. Results In our study, M:F ratio was 1.5:1 with median age 42 years, Hb - 8.0 g/dl, TLC-7900/?l, and platelet – 35000/?l and varied clinical presentation. Four patients were microgranular variants, and the rest were hypergranular. MPO and CAE positivity were100% and for NSE it was 33.33%. Molecular analysis revealed PML-RAR? isoforms of bcr1 in 42.85%, bcr2 in 14.28% and bcr3 in 38.09% patients. No correlation was found between PML-RAR? bcr isoforms, different haematological parameters and response to treatment. Conclusions Higher incidence of PML-RAR? bcr-1 isoform was found in Indian APL patients with no significant correlation between different haematological parameters and response to treatment. PMID:24455113

  11. Pathophysiology of white-nose syndrome in bats: a mechanistic model linking wing damage to mortality.

    PubMed

    Warnecke, Lisa; Turner, James M; Bollinger, Trent K; Misra, Vikram; Cryan, Paul M; Blehert, David S; Wibbelt, Gudrun; Willis, Craig K R

    2013-08-23

    White-nose syndrome is devastating North American bat populations but we lack basic information on disease mechanisms. Altered blood physiology owing to epidermal invasion by the fungal pathogen Geomyces destructans (Gd) has been hypothesized as a cause of disrupted torpor patterns of affected hibernating bats, leading to mortality. Here, we present data on blood electrolyte concentration, haematology and acid-base balance of hibernating little brown bats, Myotis lucifugus, following experimental inoculation with Gd. Compared with controls, infected bats showed electrolyte depletion (i.e. lower plasma sodium), changes in haematology (i.e. increased haematocrit and decreased glucose) and disrupted acid-base balance (i.e. lower CO2 partial pressure and bicarbonate). These findings indicate hypotonic dehydration, hypovolaemia and metabolic acidosis. We propose a mechanistic model linking tissue damage to altered homeostasis and morbidity/mortality. PMID:23720520

  12. Pathophysiology of white-nose syndrome in bats: a mechanistic model linking wing damage to mortality

    USGS Publications Warehouse

    Warnecke, Lisa; Turner, James M.; Bollinger, Trent K.; Misra, Vikram; Cryan, Paul M.; Blehert, David S.; Wibbelt, Gudrun; Willis, Craig K.R.

    2013-01-01

    White-nose syndrome is devastating North American bat populations but we lack basic information on disease mechanisms. Altered blood physiology owing to epidermal invasion by the fungal pathogen Geomyces destructans (Gd) has been hypothesized as a cause of disrupted torpor patterns of affected hibernating bats, leading to mortality. Here, we present data on blood electrolyte concentration, haematology and acid–base balance of hibernating little brown bats, Myotis lucifugus, following experimental inoculation with Gd. Compared with controls, infected bats showed electrolyte depletion (i.e. lower plasma sodium), changes in haematology (i.e. increased haematocrit and decreased glucose) and disrupted acid–base balance (i.e. lower CO2 partial pressure and bicarbonate). These findings indicate hypotonic dehydration, hypovolaemia and metabolic acidosis. We propose a mechanistic model linking tissue damage to altered homeostasis and morbidity/mortality.

  13. Current clinical efficacy of chloroquine for the treatment of Plasmodium falciparum infections in urban Dar es Salaam, United Republic of Tanzania.

    PubMed Central

    Premji, Z.; Makwaya, C.; Minjas, J. N.

    1999-01-01

    Reported is the use of a 14-day WHO protocol, which takes into account the clinical, parasitological and haematological responses to antimalarial drugs, to determine the efficacy of chloroquine in the treatment of uncomplicated malaria in young children (n = 200) in urban Dar es Salaam. Chloroquine failure was found in 43% of the children. Of these, 12.5% were considered to be early treatment failures and were given a single dose of sulfadoxine-pyrimethamine. Fever subsided in all children treated with sulfadoxine-pyrimethamine and there were no parasitological failures. In addition, children treated with sulfadoxine-pyrimethamine because of early treatment failure with chloroquine had better haematological recovery than the chloroquine-sensitive group. It is concluded that chloroquine can no longer be considered an effective therapy for P. falciparum malaria in young children in Dar es Salaam. PMID:10534897

  14. Cerebral atrophy in a vitamin B12-deficient infant of a vegetarian mother.

    PubMed

    Kocaoglu, Celebi; Akin, Fatih; Caksen, Hüseyin; Böke, Saltuk Bu?ra; Arslan, Sükrü; Aygün, Serhat

    2014-06-01

    In developed countries, vitamin B12 (cobalamin) deficiency usually occurs in children, exclusively breastfed ones whose mothers are vegetarian, causing low body stores of vitamin B12. The haematologic manifestation of vitamin B12 deficiency is pernicious anaemia. It is a megaloblastic anaemia with high mean corpuscular volume and typical morphological features, such as hyperlobulation of the nuclei of the granulocytes. In advanced cases, neutropaenia and thrombocytopaenia can occur, simulating aplastic anaemia or leukaemia. In addition to haematological symptoms, infants may experience weakness, fatigue, failure to thrive, and irritability. Other common findings include pallor, glossitis, vomiting, diarrhoea, and icterus. Neurological symptoms may affect the central nervous system and, in severe cases, rarely cause brain atrophy. Here, we report an interesting case, a 12-month old infant, who was admitted with neurological symptoms and diagnosed with vitamin B12 deficiency. PMID:25076673

  15. Time-dependent biodistribution, clearance and biocompatibility of magnetic fibrin nanoparticles: an in vivo study

    NASA Astrophysics Data System (ADS)

    Prabu, Periyathambi; Vedakumari, Weslen S.; Sastry, Thotapalli P.

    2015-05-01

    Recently, bioretention and toxicity of injected nanoparticles in the body has drawn much attention in biomedical research. In the present study, 5 mg Fe per kg body weight of magnetic fibrin nanoparticles (MFNPs) were injected into mice intravenously and investigated for their blood clearance profile, biodistribution, haematology and pathology studies for a time period of 28 days. Moderately long circulation of MFNPs in blood was observed with probable degradation and excretion into the bloodstream via monoatomic iron forms. Inductively coupled plasma optical emission spectrometry (ICP-OES) and Prussian blue staining results showed increased accumulation of MFNPs in the liver, followed by spleen and other organs. Body weight, spleen/thymus indexes, haematology, serum biochemistry and histopathology studies demonstrated that MFNPs were biocompatible. These results suggest the feasibility of using MFNPs for drug delivery and imaging applications.

  16. Persistence of neurological damage induced by dietary vitamin B-12 deficiency in infancy.

    PubMed

    von Schenck, U; Bender-Götze, C; Koletzko, B

    1997-08-01

    A case is reported of a 14 month old boy with severe dietary vitamin B-12 deficiency caused by his mother's vegan diet. Clinical, electroencephalography (EEG), and haematological findings are described. Cranial magnetic resonance imaging (MRI) showed severe frontal and frontoparietal cranial atrophy. Vitamin B-12 supplements led to a rapid improvement of haematological and neurological symptoms. Serum vitamin B-12 and urinary methylmalonate excretion were normal 10 days after treatment began. After six weeks, EEG was normal and cranial MRI after 10 weeks showed complete disappearance of all structural abnormalities. Cognitive and language development, however, remained seriously retarded at the age of 2 years. It is concluded that infantile vitamin B-12 deficiency induced by maternal vegan diets may cause lasting neurodisability even though vitamin B-12 supplementation leads to rapid resolution of cerebral atrophy and electroencephalographic abnormality. PMID:9301352

  17. Pathophysiology of white-nose syndrome in bats: a mechanistic model linking wing damage to mortality

    PubMed Central

    Warnecke, Lisa; Turner, James M.; Bollinger, Trent K.; Misra, Vikram; Cryan, Paul M.; Blehert, David S.; Wibbelt, Gudrun; Willis, Craig K. R.

    2013-01-01

    White-nose syndrome is devastating North American bat populations but we lack basic information on disease mechanisms. Altered blood physiology owing to epidermal invasion by the fungal pathogen Geomyces destructans (Gd) has been hypothesized as a cause of disrupted torpor patterns of affected hibernating bats, leading to mortality. Here, we present data on blood electrolyte concentration, haematology and acid–base balance of hibernating little brown bats, Myotis lucifugus, following experimental inoculation with Gd. Compared with controls, infected bats showed electrolyte depletion (i.e. lower plasma sodium), changes in haematology (i.e. increased haematocrit and decreased glucose) and disrupted acid–base balance (i.e. lower CO2 partial pressure and bicarbonate). These findings indicate hypotonic dehydration, hypovolaemia and metabolic acidosis. We propose a mechanistic model linking tissue damage to altered homeostasis and morbidity/mortality. PMID:23720520

  18. Hematological parameters associated with parasitism in pike, Esox lucius caught from Anzali wetland.

    PubMed

    Fallah, Farzin Jamalzad; Khara, Hossein; Rohi, Javad Daghigh; Sayadborani, Mohammad

    2015-06-01

    This study involved 120 pike, Esox lucius, captured from Anzali wetland. Parasite fauna were identified in captured fish. Also, changes of haematological parameters were compared both infected and uninfected fish. Parasitological inspections revealed the following infestations: Skin: Lernea cyprinacea, Argulus foliaceus (Crustacean) and Tricodina sp. (Ciliatea). Gill: Dactylogyrus sp. (Digenea) and Tetraonchus monenteron (Monogenea). Eye: Diplostomum spathaceum (Digenea). Gut: Eustrongylides exises, Rhipdocotyle illense, Raphidascaris acus (Nematode), Corynosoma Strumosum (Acanthocephala). Most prevalence and intensity were related to Eustrongylides exises and Rhipdocotyle illense. Following haematological parameters were evaluated: haematocrit, haemoglobin concentration, erythrocyte and leukocyte counts, mean cell volume (MCV), mean cell haemoglobin, mean cell haemoglobin concentration lymphocytes, monocytes and eosinophils. Significant difference was found for MCV between infected and uninfected fish. PMID:26064009

  19. Frequent de novo mutations of the ANK1 gene mimic a recessive mode of transmission in hereditary spherocytosis: three new ANK1 variants: ankyrins Bari, Napoli II and Anzio.

    PubMed

    Randon, J; Miraglia del Giudice, E; Bozon, M; Perrotta, S; De Vivo, M; Iolascon, A; Delaunay, J; Morle, L

    1997-03-01

    A subset of spherocytosis cases associated with mutations of the ANK1 gene present an apparently recessive inheritance pattern on a clinical and haematological basis. We identified three novel out-of-frame deletions in the ANK1 gene: allele Bari (1361delG), Napoli II (2883delC) and Anzio (3032delCA) in three Italian patients, two of whom have been splenectomized. Analysis of the cDNA showed small or trace amounts of ankyrin mRNAs in Bari, Napoli II and Anzio. The parents were normal clinically and haematologically and did not carry the mutations exhibited by their children. We confirmed the de novo character of the HS mutations based on paternity testing. Recessive HS associated with the ANK1 gene is probably rarer than initially thought, and spherocytosis may often be due to de novo mutations. PMID:9054656

  20. A double blind study to evaluate the tolerability of gadodiamide injection and its effect on renal function in patients undergoing cerebral magnetic resonance imaging.

    PubMed

    Lundby, B; Berg, K J; Lien, H H; Aamdal, S

    1993-10-01

    Gadodiamide injection was administered intravenously to 28 patients with cancer undergoing cerebral magnetic resonance imaging (MRI). Two parallel groups were used to evaluate the safety of single doses of 0.1 and 0.3 mmol per kilogram body weight (kgbw). Adverse events, vital signs, blood chemistry, haematology and urinalysis were the principal measures of safety. Four patients, all in the 0.1 mmol kgbw-1 group, experienced a total of six adverse events. No adverse events were reported in the 0.3 mmol kgbw-1 group. No clinically significant changes in blood chemistry, haematology or urinalysis occurred. No significant changes in renal tubular function or glomerular filtration rate were observed after injection at either dose. Overall, this study suggests that gadodiamide injection is a safe and effective contrast medium for use in patients undergoing cerebral MRI at both the 0.1 and 0.3 mmol kgbw-1 doses. PMID:8220968

  1. Four cases of bacteraemia caused by Fusobacterium nucleatum in febrile, neutropenic patients.

    PubMed

    Terhes, Gabriella; Piukovics, Klára; Urbán, Edit; Nagy, Elisabeth

    2011-07-01

    Although bacteraemia caused by obligate anaerobic bacteria is a rare event, this phenomenon will be an emerging problem among oncohaematological patients. We report four cases of bacteraemia caused by Fusobacterium nucleatum in febrile, neutropenic patients over a 10 month period. All patients had haematological malignancy and severe neutropenia, and three of them suffered from oral mucositis or oedema of the oral mucosal surfaces, which was the probable portal of entry. All isolated strains were susceptible to standard anti-anaerobic antibiotics. PMID:21415204

  2. Spreading and staining of human metaphase chromosomes on aminoalkylsilane-treated glass slides

    Microsoft Academic Search

    A. C. Van Prooijen-Knegt; A. K. Raap; M. J. M. van der Burg; J. Vrolijk; M. van der Ploeg

    1982-01-01

    Summary  The properties of aminoalkylsilane-treated glass slides for the preparation of metaphase spreads and their staining quality have been studied and compared with those of slides which had only been cleaned in ethanol\\/ether. The parameters investigated were: (1) the average area of metaphases from cultures of blood from both healthy donors and haematology patients; (2) the influence of the positively charged

  3. A fixed drug eruption to Baralgin (dipyrone) tablets.

    PubMed

    Pinto Pereira, L M

    1994-12-01

    Dipyrone-induced adverse skin reactions appear within the first seven days of drug administration, and the aetiology is often missed. A patient who regularly consumed dipyrone for dysmenorrhoea is presented. The adverse cutaneous manifestations may be predictive of the occurrence of severe haematological adverse reactions. Adverse drug reactions (ADR) should be documented, and initiation of ADR monitoring units in the Caribbean will be a step in establishing a data base of these events in Caribbean populations. PMID:7900381

  4. Patient participation in the medical decision-making process in haemato-oncology--a qualitative study.

    PubMed

    Ernst, J; Berger, S; Weißflog, G; Schröder, C; Körner, A; Niederwieser, D; Brähler, E; Singer, S

    2013-09-01

    Cancer patients are showing increased interest in shared decision-making. Patients with haematological illnesses, however, express considerably less desire for shared decision-making as compared with other oncological patient groups. The goal of the current project was to identify the reasons for the lower desire for shared decision-making among patients with haematological illness. We conducted qualitative, semi-structured interviews with 11 haematological patients (39-70 years old) after the beginning of therapy concerning the course and evaluation of medical shared decision-making. The patients were often overwhelmed by the complexity of the illness and the therapy and did not want to assume any responsibility in medical decision-making. They reported a great deal of distress and very traditional paternalistic role expectations with regards to their health care providers, which limited the patients' ability to partake in the decision-making process. In contrast to the socio-cultural support for many other oncological diseases, haematological diseases are not as well supported, e.g. there is a lack of self-help materials, systematic provision of information and support groups for patients, which may be related to a lower empowerment of this patient population. Results show the limits of patient participation in the context of highly complicated medical conditions. In addition to already researched preferences of the physicians and patients for shared decision-making, future research should pay greater attention to the process and other variables relevant to this aspect of the doctor-patient relationship. PMID:23731258

  5. Haematotoxicity of integrated textile mill effluent to an air-breathing fish Heteropneustes fossilis (Bloch).

    PubMed

    Murugesan, A G; Ramathilaga, A; Haniffa, M A

    2013-05-01

    The effect of integrated textile mill effluent on the haematological characteristic of the fish Heteropneustes fossilis was studied. LC50 values were found to be 34%-38% on 96-24 h duration. Four sub-lethal concentrations were selected for this study. Exposure to effluent caused an increase in large lymphocytes, monocytes, neutrophils and basiphil granulocytes and decrease in small lymphocytes in H.fossilis. PMID:23334568

  6. Analysis and quantification of prescribing and transcription errors in a paediatric oncology service

    Microsoft Academic Search

    R. Pichon; L. Vodoz; G. L. Zelger; P. Wacker; J. Humbert

    2002-01-01

    Objective:To analyse and quantify the omission errors linked to physicians' prescribing and errors linked to nurses' transcription and to identify the type and frequency of factors associated. No pharmacological factors are taken into account.Design: Analysis of physicians' prescriptions and nurses' transcriptions in chemotherapeutic treatments (20 patient files) and non-chemotherapeutic treatments (20 patient files) retrospective study.Setting:Paediatric onco-haematology unit.Main outcome measures :categorisation

  7. No correlation between estimated and actual glomerular filtration rates in pediatric oncology patients

    E-print Network

    Uzunova, Lena; Wong, Anny; Messahel, Boo; Murray, Matthew J.

    2015-03-17

    No correlation between estimated and actual glomerular filtration rates in pediatric oncology patients. Lena Uzunova1, Anny Wong1, Boo Messahel1, Matthew J. Murray1,2 1Department of Paediatric Haematology and Oncology, Addenbrooke’s Hospital... institution study comparing estimated glomerular filtration rate (eGFR) with actual GFR in children with cancer. As glomerular filtration is a key route of excretion of many chemotherapy agents, its accurate determination is essential in order to allow...

  8. Perinatal stroke: a case-based review

    Microsoft Academic Search

    Arvind Sehgal

    Neonatal stroke is a diverse clinical entity. Terminology and aetiology described in the literature are very varied. While\\u000a numerous risk factors are cited, only few case–control studies have investigated them in a systematic fashion. This equipoise\\u000a extends to the investigational and management profile of perinatal stroke too. Controversy persists about the suitability\\u000a of detailed haematological thrombophilia workup in the neonatal

  9. CYPERMETHRIN INDUCED ANAEMIA IN MALE RABBITS

    Microsoft Academic Search

    L. AHMAD; A. KHAN; M. Z. KHAN; I. HUSSAIN

    The effects on haematology of 48 rabbits (24 of each sex) were studied by administering cypermethrin (CY) intraperitoneally. In each sex, animals were divided into four equal groups A, B, C and D. The animals in groups B, C and D received low (50 mg.kg-1 b. wt.), medium (100 mg.kg-1 b. wt.) and high (150 mg.kg -1 b. wt.) CY

  10. Putative clinical piroplasmosis in a Burchell's zebra (Equus quagga burchelli).

    PubMed

    Lampen, F; Bhoora, R; Collins, N E; Penzhorn, B L

    2009-12-01

    A 10-year-old tame zebra gelding was presented after suffering from lethargy, nervousness, reported anaemia and icterus as well as a decreased appetite. These symptoms were seen over some months, with changing severity. The animal was immobilised, treated, and blood specimens were submitted for haematology and biochemistry. This report describes molecular characterisation of Theileria equi recovered from this animal, as well as the clinical findings, treatment and historical relevance of piroplasmosis in zebra in southern Africa. PMID:20458869

  11. Cytokine expression during the outcome of canine experimental infection by Leishmania infantum

    Microsoft Academic Search

    Gabriela M Santos-Gomes; Ricardo Rosa; Clara Leandro; Sofia Cortes; Pedro Romăo; Henrique Silveira

    2002-01-01

    In this study, the cytokines, interferon-gamma (IFN-?), interleukin-2 (IL-2), IL-12 p40, IL-6 and IL-10, expressed by peripheral blood mononuclear cells of 13 beagle dogs inoculated with Leishmania infantum amastigotes, were analysed during a period of up to 23 months. The course of infection was monitored through clinical and parasitological examinations, haematological alterations and serum antileishmania antibody levels. Dogs developed symptomatic

  12. Near fatal acute colchicine intoxication in a child. A case report

    Microsoft Academic Search

    Aviv Goldbart; Shaul Sofer; Joseph Kapelushnik

    2000-01-01

    An 8-year-old child with familial Mediterranean fever exhibited signs of colchicine intoxication while receiving prophylactic\\u000a doses of the drug. She developed gastro-intestinal, central nervous system, cardiovascular and haematological disturbances.\\u000a Over 2 months she had been drinking high doses of natural grapefruit juice which, combined with long-term colchicine therapy\\u000a and a viral upper respiratory tract infection, increased her susceptibility to the

  13. Histological and Molecular Classification of Chronic Myeloproliferative Disorders in the Age of JAK2: Persistence of Old Questions despite New Answers

    Microsoft Academic Search

    Kais Hussein; Oliver Bock; Hans Kreipe

    2007-01-01

    The discovery of the Janus kinase 2 gain-of-function V617F mutation (JAK2V617F) provided a major breakthrough in the understanding of Philadelphia chromosome-negative chronic myeloproliferative disorders (Ph– CMPD). Among haematologic neoplasm the mutation appears to be almost specific for Ph– CMPD but the different entities comprising polycythaemia vera (PV), essential thrombocythaemia and chronic idiopathic myelofibrosis (CIMF) are not discriminated by the mutation.

  14. Evaluation of chlorhexidine and silver-sulfadiazine impregnated central venous catheters for the prevention of bloodstream infection in leukaemic patients: a randomized controlled trial

    Microsoft Academic Search

    C. Logghe; Ch. Van Ossel; W. D'Hoore; H. Ezzedine; G. Wauters; J. J. Haxhe

    1997-01-01

    It has been suggested that central venous catheters impregnated with antiseptics such as chlohexidine and silver-sulfadiazine reduce the risk of catheter-related bacteraemia in intensive care patients. Patients suffering from haematologic malignancy treated by chemotherapy through a central venous catheter are at even greater sisk of catheter-related bacteraemia. A prospective double-blind randomized controlled trial was performed in order to investigate the

  15. Epoetin alfa in platinum-treated ovarian cancer patients: results of a multinational, multicentre, randomised trial

    Microsoft Academic Search

    P M Wilkinson; M Antonopoulos; M Lahousen; M Lind; P Kosmidis

    2006-01-01

    This multicentre, open-label, controlled clinical trial assessed the effects of epoetin alfa treatment on haematologic and quality of life (QOL) parameters in 182 anaemic (Hb?12 g dl?1) ovarian cancer patients receiving platinum chemotherapy. Patients were randomised 2 : 1 to receive epoetin alfa 10 000–20 000 IU three times weekly plus best standard treatment (BST) or BST only. Main study

  16. For better or for worse: the role of Pim oncogenes in tumorigenesis

    Microsoft Academic Search

    Martijn C. Nawijn; Andrej Alendar; Anton Berns

    2010-01-01

    Pim oncogenes are overexpressed in a wide range of tumours from a haematological and epithelial origin. Pim genes encode serine\\/threonine kinases that have been shown to counteract the increased sensitivity to apoptosis induction that is associated with MYC-driven tumorigenesis. Recently, considerable progress has been made in characterizing the pathways of PIM-mediated survival signalling. Given the unique structure of their active

  17. EANM procedure guideline for 32 P phosphate treatment of myeloproliferative diseases

    Microsoft Academic Search

    Jan Tennvall; Boudewijn Brans

    2007-01-01

    Introduction  \\u000a 32P phosphate was the first therapeutic radioisotope, used in leukaemia about 70 years ago. Since then, many new agents for\\u000a haematological proliferations have been introduced successfully. Today there remains a distinct subgroup of elderly patients\\u000a with polycythaemia vera and essential thrombocythaemia for whom 32P is the most optimal treatment option, an assertion supported by two large studies with long follow-up.

  18. Effects of Epidural Ketamine–Xylazine Combination on the Clinicophysiological and Haematobiochemical Parameters of Uraemic and Healthy Goats

    Microsoft Academic Search

    K. Singh; P. Kinjavdekar; Amarpal; H. P. Aithal; A. Gopinathan; G. R. Singh; A. M. Pawde; K. Pratap

    2007-01-01

    Xylazine–ketamine combination was evaluated for its efficacy and safety after epidural administration in uraemic and healthy\\u000a goats. The combination (xylazine 0.025 mg\\/kg and ketamine 2.5 mg\\/kg) was administered to uraemic (n = 6) and healthy (n = 6) animals in the lumbosacral epidural space. The combination was evaluated in terms of clinical, physiological, haematological\\u000a and biochemical parameters. The onset of

  19. Long-term results of NOPHO ALL92 and ALL2000 studies of childhood acute lymphoblastic leukemia

    Microsoft Academic Search

    K Schmiegelow; E Forestier; M Hellebostad; M Heyman; J Kristinsson; S Söderhäll; M Taskinen

    2010-01-01

    Analysis of 2668 children with acute lymphoblastic leukemia (ALL) treated in two successive Nordic clinical trials (Nordic Society of Paediatric Haematology and Oncology (NOPHO) ALL-92 and ALL-2000) showed that 75% of all patients are cured by first-line therapy, and 83% are long-term survivors. Improvements in systemic and intrathecal chemotherapy have reduced the use of central nervous system (CNS) irradiation to

  20. The effects of naturally deoxynivalenol-contaminated oats on the clinical condition, blood parameters, performance and carcass composition of growing pigs

    Microsoft Academic Search

    B. Bergsjř; W. Langseth; I. Nafstad; J. Hřgset Jansen; H. J. S. Larsen

    1993-01-01

    A feeding trial with naturally deoxynivalenol (DON)-contaminated oats included in feed mixtures at graded levels was conducted in growing pigs. The DON concentrations were 0, 0.7, 1.7, and 3.5 mg\\/kg of complete feed mixture givenad libitum to different groups. The data recorded were feed consumption, body weight gain, slaughter weight, biochemical and haematological data including serum immunoglobulin A, clinical condition

  1. Heinz body haemolytic anaemia in a dog secondary to ingestion of a zinc toy: A case report

    Microsoft Academic Search

    Nicholas Bexfield; Joy Archer; Michael Herrtage

    2007-01-01

    A 6-year-old Labrador retriever was referred for investigation of severe lethargy and suspected immune-mediated haemolytic anaemia. Clinical examination revealed pale mucous membranes and jaundice. Haematology demonstrated large numbers of Heinz bodies and a marked anaemia, which was strongly regenerative. Serum zinc concentrations were markedly elevated. Analysis of a metal toy vomited by the dog 3 days prior to presentation revealed

  2. Epidemiology of Invasive Pulmonary Aspergillosis

    Microsoft Academic Search

    Carol A. Kauffman; Nelson P. Nicolasora

    \\u000a The epidemiology of IA, the major invasive mould infection in immunocompromised patients, has evolved over the last several\\u000a decades. During the 1990s, increasing morbidity and mortality from these infections, particularly amongst the increasing numbers\\u000a of patients being treated for haematological malignancies and those undergoing allogeneic haematopoietic stem cell transplantation,\\u000a became a universal experience in many tertiary care medical centres. Changes

  3. Sweet syndrome: a lesson in the management of the cutaneous lesions of malignancy.

    PubMed

    Leighton, Philippa; Amirfeyz, Rouin

    2015-01-01

    We present the case of a lady with rapidly growing haemorraghic bullae in the digits of both hands. There was no effect of antibiotics and surgical debridement was detrimental. The lesions regressed following steroid therapy. We discuss the cutaneous complications of haematological malignancies. We emphasize the importance of avoiding surgery in these cases and explain that immunosuppressive therapy should form the basis of treatment. PMID:25672975

  4. Pegfilgrastim compared with filgrastim after autologous peripheral blood stem cell transplantation in patients with solid tumours and lymphomas

    Microsoft Academic Search

    Alberto Ballestrero; Davide Boy; Roberta Gonella; Maurizio Miglino; Marino Clavio; Valentina Barbero; Alessio Nencioni; Marco Gobbi; Franco Patrone

    2008-01-01

    To evaluate the safety and efficacy of pegfilgrastim administered as haematological support after autologous peripheral blood\\u000a stem cell transplantation, we compared 44 patients with solid tumours and lymphomas receiving a 6-mg single dose of pegfilgrastim\\u000a on day +5 after transplantation to a historical control group of 25 patients receiving filgrastim 5 ?g kg?1 day?1 starting on day +5. There were no

  5. Expression of basic fibroblast growth factor is associated with poor outcome in non-Hodgkin's lymphoma

    Microsoft Academic Search

    I Pazgal; Y Zimra; C Tzabar; E Okon; E Rabizadeh; M Shaklai; O Bairey

    2002-01-01

    It is now clear that angiogenesis and angiogenesis factors are important in the pathogenesis of haematological malignancies. High pretreatment levels of serum basic fibroblast growth factor have been shown to be associated with poor prognosis in patients with non-Hodgkin's lymphoma. The aim of this study was to evaluate whether non-Hodgkin's lymphoma cells express basic fibroblast growth factor and\\/or its receptor

  6. Transcoelomic spread and metastasis of a squamous cell carcinoma of presumed pancreatic duct origin in a cat

    Microsoft Academic Search

    RC Gebbie; SA Hunter; AC Hartman

    2011-01-01

    CASE HISTORY: A 13-year-old female spayed domestic shorthaired cat was examined because of lethargy, inappetance and weight loss.CLINICAL FINDINGS: No clinically significant haematological or biochemical abnormalities were detected, but an abdominal mass was palpated. Abdominal examination using ultrasonography revealed soft tissue masses in the cranial abdomen, involving the spleen, as well as the liver and abdominal wall; the pancreas was

  7. Transcoelomic spread and metastasis of a squamous cell carcinoma of presumed pancreatic duct origin in a cat

    Microsoft Academic Search

    RC Gebbie; SA Hunter; AC Hartman

    2012-01-01

    CASE HISTORY: A 13-year-old female spayed domestic shorthaired cat was examined because of lethargy, inappetance and weight loss.CLINICAL FINDINGS: No clinically significant haematological or biochemical abnormalities were detected, but an abdominal mass was palpated. Abdominal examination using ultrasonography revealed soft tissue masses in the cranial abdomen, involving the spleen, as well as the liver and abdominal wall; the pancreas was

  8. Biostatistical evidence for two distinct chronic open angle glaucoma populations

    Microsoft Academic Search

    M Schulzer; S M Drance; C J Carter; D E Brooks; G R Douglas; W Lau

    1990-01-01

    Twenty-six eyes of 26 patients with low-tension glaucoma and 34 eyes of 34 patients with high-tension glaucoma were studied. Fifty-one measurements were available on each patient, including visual field indices, finger blood flow measurements, as well as haematological, coagulation, and biochemical and rheological variables. Multivariate analysis revealed two statistically distinct groups of patients, with low and high tension glaucoma cases

  9. Current markers of the Athlete Blood Passport do not flag microdose EPO doping

    Microsoft Academic Search

    Michael Ashenden; Clare E. Gough; Andrew Garnham; Christopher J. Gore; Ken Sharpe

    The Athlete Blood Passport is the most recent tool adopted by anti-doping authorities to detect athletes using performance-enhancing\\u000a drugs such as recombinant human erythropoietin (rhEPO). This strategy relies on detecting abnormal variations in haematological\\u000a variables caused by doping, against a background of biological and analytical variability. Ten subjects were given twice weekly\\u000a intravenous injections of rhEPO for up to 12 weeks.

  10. Primary invasive aspergillosis with disseminated intravascular coagulation as a presenting feature of non-Hodgkin's lymphoma.

    PubMed

    Balsitis, Margaret; Elgoweini, Maha; Martin, Sarah J; Shankland, Gillian S; Paxton, Jane; Bal, Abhijit M

    2015-09-01

    Invasive aspergillosis (IA) is a life-threatening infection. IA is usually seen in severely immunocompromised patients. However, IA as a presenting feature of non-Hodgkin's lymphoma is rare. The patient we describe had no signs or symptoms of lymphoma prior to hospital admission. A. fumigatus was isolated from respiratory tract specimens on the day of admission and fungal elements were detected on autopsy. Isolation of Aspergillus in patients with severe sepsis should trigger a search haematological malignancy. PMID:26199867

  11. Evaluation of ultra-short dialysis

    Microsoft Academic Search

    J A Trafford; P Sharpstone; R Evans; R Ireland

    1979-01-01

    A study was carried out to compare the effects of three dialysis regimens of different durations. Nineteen patients dialysed over 1975--7 for an average of only 11.2 hours per week were compared with nine dialysed for 24 hours per week (1974--6) and 10 dialysed for 27 hours (1971--3). Clinical, biochemical, and haematological results showed that the short dialysis regimen was

  12. A complementary component to atypical haemolytic uraemic syndrome

    PubMed Central

    Gandhi, Vanita; Burns, Aine; Goodship, Timothy

    2013-01-01

    We describe a case of a 22-year-old man who presented with clinical features of atypical haemolytic uraemic syndrome and in whom genetic analysis of complement regulatory proteins demonstrated a CD46 mutation. He was treated with plasma exchange, intermittent haemodialysis and blood transfusions. At 6-month follow-up his haematological parameters and renal function showed complete resolution, and he experienced no further disease recurrence in the subsequent year. PMID:23780777

  13. T-cell lymphocytic leukaemia and lymphoma in a horse

    Microsoft Academic Search

    JC Thompson

    2003-01-01

    ExtractA 10-year-old Thoroughbred gelding showed rapid weight loss over about 1 week. Clinically, it had increased respiration and heart rates, dark red mucous membranes with petechial haemorrhages, and a bilateral keratitis. Routine haematological examination showed a lymphocytosis with increased numbers of small hyperchromatic lymphocytes in circulation, but no anaemia. On necropsy, many enlarged abdominal lymph nodes were seen and there

  14. Ancillary techniques in bone marrow pathology: molecular diagnostics on bone marrow trephine biopsies

    Microsoft Academic Search

    Falko Fend; Oliver Bock; Markus Kremer; Katja Specht; Leticia Quintanilla-Martinez

    2005-01-01

    Pathologic examination of trephine bone marrow (BM) biopsies plays a central role in the diagnosis and staging of haematological\\u000a neoplasms and other disorders affecting haematopoiesis. Haematopathology has been profoundly influenced by the advent of molecular\\u000a genetic techniques suitable for paraffin-embedded tissues, and certain applications, such as the determination of B- and T-cell\\u000a clonality, belong to its standard diagnostic repertoire. Many

  15. Exercise?induced muscle cramp: A prospective biochemical study in marathon runners

    Microsoft Academic Search

    R. J. Maughan

    1986-01-01

    Exercise?induced muscle cramp has been considered to result from disturbances of fluid and electrolyte balance resulting from excessive sweat loss. Serum biochemical and haematological measurements were made on 82 male marathon runners before and after a 42.2?km race. Fifteen (18%) of the runners reported an attack of muscle cramp which occurred after 35 ± 6 km (mean±s.d.) had been covered.

  16. Primary invasive aspergillosis with disseminated intravascular coagulation as a presenting feature of non-Hodgkin's lymphoma

    PubMed Central

    Balsitis, Margaret; Elgoweini, Maha; Martin, Sarah J; Shankland, Gillian S.; Paxton, Jane; Bal, Abhijit M

    2015-01-01

    Invasive aspergillosis (IA) is a life-threatening infection. IA is usually seen in severely immunocompromised patients. However, IA as a presenting feature of non-Hodgkin's lymphoma is rare. The patient we describe had no signs or symptoms of lymphoma prior to hospital admission. A. fumigatus was isolated from respiratory tract specimens on the day of admission and fungal elements were detected on autopsy. Isolation of Aspergillus in patients with severe sepsis should trigger a search haematological malignancy. PMID:26199867

  17. Experimental Tribulus terrestris poisoning in goats

    Microsoft Academic Search

    M. R. Aslani; A. R. Movassaghi; M. Mohri; V. Ebrahim-pour; A. N. Mohebi

    2004-01-01

    Seven, 1–2-year-old native goats were fed dried Tribulus terrestris from Sabzevar district of Khorasan province for 8 weeks. Two goats showed clinical signs of toxicity including weight loss, depression, ruminal stasis, icterus and elevation of body temperature. Haematological and biochemical trails revealed a declining of packed cell volume (PCV) and plasma total protein and elevation of total and direct bilirubin,

  18. Chelates and Stability

    Microsoft Academic Search

    Sameh K. Morcos

    Extracellular gadolinium-based MRI contrast media are all chelates containing Gd ions (Gd3+). Free gadolinium is highly toxic and can cause splenic degeneration, central lobular necrosis of the liver, enzyme inhibition,\\u000a calcium channel blocking and a variety of haematological abnormalities (\\u000a Dawson 1999; \\u000a Desreux and Gilsoul 1999). Therefore, it is crucially important that Gd3+ should be strongly attached to a chelate

  19. Intravenous immune globulin in lysinuric protein intolerance

    Microsoft Academic Search

    C. Dionisi-Vici; L. De Felice; M. El Hachem; S. Bottero; C. Rizzo; A. Paoloni; B. Goffredo; G. Sabetta; M. Caniglia

    1998-01-01

    In addition to systemic manifestations with skeletal, pulmonary, renal, and haematological signs, lysinuric protein intolerance (LPI), a membrane transport defect of cationic amino acids, is often complicated by severe life-threatening immunological manifestations. A 10-year-old boy with LPI who exhibited a severe systemic immunohaematological disease is described here. This patient showed cutaneous lesions similar to the subacute form of systemic lupus

  20. The prevention and treatment of cytomegalovirus infection in haematopoietic stem cell transplantation

    Microsoft Academic Search

    Mark Tuthill; Frederick Chen; Samantha Paston; Hugo De La Peńa; Sylvie Rusakiewicz; Alejandro Madrigal

    2009-01-01

    Allogeneic haematopoietic stem cell transplantation (HSCT) is an intensive medical treatment involving myeloablative chemo-radiotherapy\\u000a followed by stem cell rescue using allogeneic haematopoietic stem cells harvested from HLA-matched donors, which is primarily\\u000a used for the treatment of haematological malignancies. Cytomegalovirus (CMV) infection is one of the major causes of morbidity\\u000a and death after HSCT. This focused research review highlights the advances

  1. Pathogenic mechanisms of caprine arthritis-encephalitis virus

    Microsoft Academic Search

    E. G. Mdurvwa; P. O. Ogunbiyi; H. S. Gakou; P. G. Reddy

    1994-01-01

    Goats infected with caprine arthritis-encephalitis virus (CAEV) show chronic arthritis and cachexia, which are progressive in nature. The immunopathogenic mechanisms responsible for these progressive clinical symptoms have not been fully elucidated. Various haematological and immunological parameters were evaluated in experimentally-infected goats showing typical signs of CAEV-induced disease. Infected goats showed recurrent lymphocytosis that may be due to constant presentation of

  2. Dose escalation study of rhenium-186 hydroxyethylidene diphosphonate in patients with metastatic prostate cancer

    Microsoft Academic Search

    John M. H. Klerk; Bernard A. Zonnenberg; Alfred D. Schip; Aalt Dijk; Shiuw H. Han; Jacques M. S. R. Quirijnen; Geert H. Blijham; Peter R Rijk

    1994-01-01

    Rhenium-186 hydroxyethylidene diphosphonate (186Re-HEDP) has been used for the palliative treatment of metastatic bone pain. A phase 1 dose escalation study was performed using 186Re-HEDP Twenty-four patients with hormone-resistant prostate cancer entered the study. Each patient had at least four bone metastases and adequate haematological function. Groups of at least three consecutive patients were treated with doses starting at 1295

  3. Bmi1 is essential for cerebellar development and is overexpressed in human medulloblastomas

    Microsoft Academic Search

    Carly Leung; Merel Lingbeek; Olga Shakhova; James Liu; Ellen Tanger; Parvin Saremaslani; Maarten van Lohuizen; Silvia Marino

    2004-01-01

    Overexpression of the polycomb group gene Bmi1 promotes cell proliferation and induces leukaemia through repression of Cdkn2a (also known as ink4a\\/Arf) tumour suppressors. Conversely, loss of Bmi1 leads to haematological defects and severe progressive neurological abnormalities in which de-repression of the ink4a\\/Arf locus is critically implicated. Here, we show that Bmi1 is strongly expressed in proliferating cerebellar precursor cells in

  4. Hepatitis B transmission from contaminated cryopreservation tank

    Microsoft Academic Search

    R. S Tedder; M. A Zuckerman; N. S Brink; A. H Goldstone; A Fielding; S Blair; K. G Patterson; A. E Hawkins; A. M Gormon; J Heptonstall; D Irwin

    1995-01-01

    Over a 25-month period, six multiply transfused patients undergoing cytotoxic treatment for haematological or other malignant disorders developed icteric acute hepatitis B virus (HBV) infection. Bone marrow or peripheral-blood stem cells had been harvested from all six patients and stored in the same cryopreservation tank for possible future transplantation. Human DNA, HBsAg, and HBV DNA with sequences identical to those

  5. Routine versus clinically driven laboratory monitoring of HIV antiretroviral therapy in Africa (DART): a randomised non-inferiority trial

    Microsoft Academic Search

    2010-01-01

    Methods In this open, non-inferiority trial in three centres in Uganda and one in Zimbabwe, 3321 symptomatic, ART- naive, HIV-infected adults with CD4 counts less than 200 cells per ?L starting ART were randomly assigned to laboratory and clinical monitoring (LCM; n=1659) or clinically driven monitoring (CDM; n=1662) by a computer- generated list. Haematology, biochemistry, and CD4-cell counts were done

  6. Deferasirox chelation therapy in patients with transfusion-dependent MDS: a 'real-world' report from two regional Italian registries: Gruppo Romano Mielodisplasie and Registro Basilicata.

    PubMed

    Maurillo, Luca; Breccia, Massimo; Buccisano, Francesco; Voso, Maria Teresa; Niscola, Pasquale; Trapč, Giulio; Tatarelli, Caterina; D'Addosio, Ada; Latagliata, Roberto; Fenu, Susanna; Piccioni, Anna Lina; Fragasso, Alberto; Aloe Spiriti, Maria A; Refrigeri, Marco; Criscuolo, Marianna; Musto, Pellegrino; Venditti, Adriano

    2015-07-01

    Deferasirox (DFX) is an orally administered iron chelator approved for use in patients with transfusion-dependent iron overload due to myelodysplastic syndromes (MDS). The safety and efficacy of DFX has been explored in clinical trial settings, but there is little data on unselected patients with MDS. The aim of this study was to retrospectively evaluate the safety, compliance, efficacy and effect on haematopoiesis of DFX in a large 'real-world' MDS population. One hundred and eighteen patients with transfusion-dependent MDS were treated with DFX across 11 centres in Italy. Serum ferritin levels, haematological response, dosing, adverse events and transfusion dependence were recorded at baseline, 3, 6, 12 and 24 months following initiation of treatment. DFX reduced mean serum ferritin levels from 1790 to 1140 ng/mL (P < 0.001), with 7.1% of patients achieving transfusion independence. Significant haematological improvement was seen in erythroid (17.6%), platelet (5.9%) and neutrophil counts (7.1%). Adverse events were reported in 47.5% of patients, including gastrointestinal and renal toxicity. Regression analysis showed that higher starting doses of DFX are associated with transfusion independence at 24 months. DFX is a safe, effective treatment for transfusion-dependent MDS that can lead to transfusion independence and haematological improvement in a subset of patients. PMID:25764148

  7. Diagnosis of the Haemoglobinopathies

    PubMed Central

    Trent, Ronald J A

    2006-01-01

    Despite having been extensively studied at both the biochemical, haematological and molecular levels, the haemoglobinopathies continue to provide a diagnostic challenge particularly in the multiethnic communities seen in Australia. Early detection and characterisation of the haemoglobinopathies is essential so that appropriate counselling can be provided to couples and families who may be at risk of severe haematological consequences. Although DNA diagnostics have made a major impact on our understanding and detection of the haemoglobinopathies, DNA mutation testing should never be considered a short cut or the test of first choice in the workup of a haemoglobinopathy. A careful three tier approach involving: (1) Full blood count (2) Special haematological tests, followed by (3) DNA mutation analysis, provides the most effective way in which to detect primary gene mutations as well as gene-gene interactions that can influence the overall phenotype. Just as important as the laboratory investigations is the family work up. Often, the first and most helpful clue to gene gene interactions comes from the family study. In Australia, there are many different forms of ? and ? thalassaemia. Increasingly, different Hb Variants are being detected, and their effects per se, or in combination with the thalassaemias, provide additional diagnostic challenges. PMID:16886045

  8. A survey of feline leukaemia virus infection of domestic cats from selected areas in Harare, Zimbabwe.

    PubMed

    Muchaamba, Francis; Mutiringindi, Takudzwa H; Tivapasi, Musavenga T; Dhliwayo, Solomon; Matope, Gift

    2014-01-01

    A cross-sectional study was conducted to detect the feline leukaemia virus (FeLV) p27 antigen and to determine risk factors and the haematological changes associated with infection in domestic cats in Zimbabwe. Sera were collected for detection of the p27 antigen, urea, creatinine, alanine aminotransferase and gamma-glutamyl transferase levels, whilst whole blood was collected for haematology. FeLV p27 antigen was detected using a rapid enzyme-linked immunosorbent assay (ELISA) test kit. Data on risk factors were analysed using a logistic regression model. Of the 100 cats tested, 41% (95% CI: 31.19% - 50.81%) (41/100) were positive for the FeLV p27 antigen. Sex and health status of cats were not significantly (p > 0.05) associated with infection. Intact cats (OR = 9.73), those living in multicat housing (OR = 5.23) and cats that had access to outdoor life (OR = 35.5) were found to have higher odds of infection compared with neutered cats, those living in single-cat housing, and without access to outdoor life, respectively. Biochemistry and haematology revealed no specific changes. The results showed that FeLV infection was high in sampled cats, providing evidence of active infection. Thus, it would be prudent to introduce specific control measures for FeLV infection in Zimbabwe. PMID:25686080

  9. Retrospective study of clinical and hematological aspects associated with dogs naturally infected by Hepatozoon canis in Ludhiana, Punjab, India

    PubMed Central

    Chhabra, Sushma; Uppal, Sanjeev Kumar; Singla, Lachhman Das

    2013-01-01

    Objective To evaluate clinical and hematological aspects of dogs naturally infected with Hepatozoon canis (H. canis) presented at the Small Animal Clinics of Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana. Methods Blood films of 34 naturally infected dogs were examined for haematological alterations and parasitaemia. Signalment and clinical signs were recorded from the animals. Clinical histories were filled out during the consultation. Results Of the 34 positive dogs by Giemsa stained peripheral blood films, 88.23% presented parasitaemia by H. canis only, while 11.77% had the combination of H. canis, Babesia sp. and Ehrlichia sp. Young male dogs less than one-year-old, of non-descript breed, were the most commonly affected. And 26.47% were presented with anorexia/inappetence as the only clinical symptom. Other clinical symptoms were mild to moderate fever, pale mucosae and lethargy; a few were also showing the signs of vomiting and diarrhoea. Haematological alterations showed mainly normochromic-normocytic anaemia, leukocytosis and neutrophilia. Conclusions The findings of this study substantiate that H. canis caused clinical and haematological alterations of the varied intensity in dogs, even with low parasitaemia, should be taken into consideration. PMID:23730562

  10. Epidemiology of candidaemia in a tertiary care university hospital: 10-year experience with 381 candidaemia episodes between 2001 and 2010.

    PubMed

    Alp, Sehnaz; Arikan-Akdagli, Sevtap; Gulmez, Dolunay; Ascioglu, Sibel; Uzun, Omrum; Akova, Murat

    2015-08-01

    Defining the epidemiology of and risk factors for candidaemia is necessary to guide empirical treatment. The objectives of this study were to determine the ranking of Candida among positive blood cultures, to define the epidemiology of candidaemia and to investigate patient characteristics and their relationship with C. albicans vs. non-albicans Candida (NAC) candidaemia. Candidaemia episodes between January 2001 and December 2010 were evaluated retrospectively. Patient characteristics were compared across Candida species. Candida ranked as the fifth most frequently isolated pathogen. Among 381 candidaemia episodes, 58.3% were due to C. albicans, followed by C. parapsilosis (15.2%), C. tropicalis (13.4%) and C. glabrata (6.8%). No statistically significant difference was observed in the distribution of C. albicans vs. NAC (P = 0.432). Patients with NAC had significantly higher rates of haematological disorders (P < 0.001) and neutropenia (P = 0.003), and were older (P = 0.024) than patients with C. albicans, whereas patients with urinary catheters had higher rates of C. albicans (P = 0.007). On species basis, C. tropicalis was more frequently isolated from patients with haematological disorders (P < 0.001) and neutropenia (P = 0.008). Patients with urinary catheters were less likely to have C. parapsilosis (P = 0.043). C. glabrata was most prevalent among patients with solid organ tumours (P = 0.038), but not evident in patients with haematological disorders. Local epidemiological features and risk factors may have important implications for the management of candidaemia. PMID:26155849

  11. Splenectomy in a general hospital.

    PubMed Central

    Glass, J M; Gilbert, J M

    1996-01-01

    Splenectomy is often performed in patients with malignant disease or trauma who are at a high risk of complications. In the long term, it increases the risk of infection by encapsulated bacteria. An audit was performed to determine the reasons for splenectomy in a district general hospital, to review the results and complications of surgery, and to see how often the patients were prescribed antibacterial prophylaxis. Twenty-eight patients underwent splenectomy in 3 years. The indication was haematological disease in 13 and trauma in four. In the remaining nine the spleen was removed either as part of a radical gastrectomy or during some other abdominal procedure. Six of the 28 patients had died, one within 30 days from disseminated intravascular coagulopathy following an emergency gastrectomy and splenectomy for haematemesis, two from progressive haematological malignant disease, two from non-haematological malignancy, and one from bronchopneumonia. Of the nine patients (32%) with complications, three required a further laparotomy. Most patients had been prescribed pneumococcal vaccine (85%) and prophylactic antibiotics (93%). PMID:8676317

  12. Evaluation of antiarthritic activity of Strychnos potatorum Linn seeds in Freund's adjuvant induced arthritic rat model

    PubMed Central

    2010-01-01

    Background Strychnos potatorum Linn (Loganiaceae) is a moderate sized tree found in southern and central parts of India, Sri Lanka and Burma. In traditional system of medicine, Strychnos potatorum Linn seeds were used for various ailments including inflammation, diabetes etc. To investigate the folkloric use of the seeds the present study was carried out on Freund's adjuvant induced arthritic rats. Methods The present study states the effect of the aqueous extract (SPE) and the whole seed powder (SPP) of Strychnos potatorum Linn seeds on the Freund's complete adjuvant (FCA) induced arthritic rat paw edema, body weight changes and alterations in haematological and biochemical parameters in both developing and developed phases of arthritis. Histopathology of proximal interphalangeal joints and radiology of hind legs were studied. Results In FCA induced arthritic rats, there was significant increase in rat paw volume and decrease in body weight increment, whereas SPP and SPE treated groups, showed significant reduction in paw volume and normal gain in body weight. The altered haematological parameters (Hb, RBC, WBC and ESR) and biochemical parameters (blood urea, serum creatinine, total proteins and acute phase proteins) in the arthritic rats were significantly brought back to near normal by the SPP and SPE treatment at the dose of 200 mg/kg/p.o in both developing and developed phases of arthritis. Further the histopathological and radiological studies revealed the antiarthritic activity of SPP and SPE by indicating fewer abnormalities in these groups when compared to the arthritic control group. Conclusion In conclusion, both SPP and SPE at the specified dose level of 200 mg/kg, p.o. showed reduction in rat paw edema volume and it could significantly normalize the haematological and biochemical abnormalities in adjuvant induced arthritic rats in both developing and developed phases of FCA induced arthritis. Further the histopathological and radiological studies confirmed the antiarthritic activity of SPP and SPE. PMID:20939932

  13. Neuropathy and paraproteins: review of a complex association.

    PubMed

    Rajabally, Y A

    2011-11-01

    Coexistence of neuropathy and monoclonal gammopathy represents a common but complex problem in clinical practice. This association is here reviewed considering latest available literature. The association is not infrequent, and various possible syndromes need to be distinguished. However, coincidental co-occurrence also needs to be recognized. The monoclonal gammopathy may be a 'monoclonal gammopathy of uncertain significance' (MGUS) or occur in a context of malignancy such as multiple myeloma or Waldenström's macroglobulinaemia. IgM paraproteins can bind to myelin-associated glycoprotein (MAG) in peripheral nerve. In this case, the paraprotein is directly linked to the neuropathy, causing a specific phenotype. One randomized controlled trial of this ('Anti-MAG') neuropathy showed possible moderate effect of rituximab on disability. Results of another trial are awaited. IgM/G/A paraproteins can be associated with a polyneuropathy indistinguishable from chronic inflammatory demyelinating polyneuropathy. Axonal neuropathies may coexist with IgM/G/A MGUS. There is insufficient evidence about causality or effective treatment in such cases. Pain/dysautonomia with an axonal neuropathy and serum paraprotein raises the possibility of amyloidosis. Specific haematological treatment is required for malignant disorders, although caution is required with neurotoxic agents. Polyneuropathy, organomegaly, endocrinopathy, M-protein, skin changes syndrome and chronic ataxic neuropathy with ophthalmoplegia, M-protein, cold agglutinins and disialosyl antibodies represent rare separate entities for which evidence-based treatment options are still lacking. The association of monoclonal gammopathy and neuropathy requires the appropriate neurological/haematological investigations for a precise diagnosis. Causality is only established in few cases. Adequate management ideally requires joint neurological/haematological input for diagnosis, monitoring and treatment. PMID:21418441

  14. National survey on intra-laboratory turnaround time for some most common routine and stat laboratory analyses in 479 laboratories in China

    PubMed Central

    Fei, Yang; Zeng, Rong; Wang, Wei; He, Falin; Zhong, Kun

    2015-01-01

    Introduction To investigate the state of the art of intra-laboratory turnaround time (intra-TAT), provide suggestions and find out whether laboratories accredited by International Organization for Standardization (ISO) 15189 or College of American Pathologists (CAP) will show better performance on intra-TAT than non-accredited ones. Materials and methods 479 Chinese clinical laboratories participating in the external quality assessment programs of chemistry, blood gas, and haematology tests organized by the National Centre for Clinical Laboratories in China were included in our study. General information and the median of intra-TAT of routine and stat tests in last one week were asked in the questionnaires. Results The response rate of clinical biochemistry, blood gas, and haematology testing were 36% (479 / 1307), 38% (228 / 598), and 36% (449 / 1250), respectively. More than 50% of laboratories indicated that they had set up intra-TAT median goals and almost 60% of laboratories declared they had monitored intra-TAT generally for every analyte they performed. Among all analytes we investigated, the intra-TAT of haematology analytes was shorter than biochemistry while the intra-TAT of blood gas analytes was the shortest. There were significant differences between median intra-TAT on different days of the week for routine tests. However, there were no significant differences in median intra-TAT reported by accredited laboratories and non-accredited laboratories. Conclusions Many laboratories in China are aware of intra-TAT control and are making effort to reach the target. There is still space for improvement. Accredited laboratories have better status on intra-TAT monitoring and target setting than the non-accredited, but there are no significant differences in median intra-TAT reported by them.

  15. Interpretation of the full blood count in systemic disease--a guide for the physician.

    PubMed

    Leach, M

    2014-01-01

    The full blood count (FBC) is perhaps the single most common investigation performed in medical patients. It has the potential, when interpreted carefully and in relation to the clinical history, to provide very useful information to assist in diagnosis and management. Clinicians are often alerted to the presence of a primary haematological disorder by abnormalities in the FBC. For the purpose of this review these diseases will not be discussed in detail but the reader will be alerted to pointers which might indicate primary blood disorders throughout the text. The haematology laboratory in large teaching hospitals will often provide up to 1,500 automated FBC analyses each day. These are individually checked for 'flags' provided by the analyser which indicate values outside the normal range. It is clearly essential that clinical information is provided with the request as this will influence how the result is handled by scientific and medical staff. Furthermore, significant abnormalities will generate a blood film request and the report will be most useful when interpreted in light of the patient's working diagnosis. In cases where a diagnosis is not yet known, even brief information on presentation, for example 'collapse with hypotension', 'fever on return to UK', 'weight loss and anorexia', can all be important and help the lab provide clinicians with guidance. This short review aims to provide physicians with a workable guide to the interpretation of some of the commoner findings in the full blood count. Some of these will be very familiar to you but some will not. This review is not meant to be exhaustive as the rare minutiae will obscure the essential core material. Your haematology colleagues are always happy to help and available for assistance in difficult or problematic cases. I have not specified normal ranges in relation to each entity as these will be defined by your local laboratory. PMID:24995446

  16. Brief Tale of a Bacteraemia by Rhodococcus equi, With Concomitant Lung Mass: What Came First, the Chicken or The Egg?

    PubMed Central

    Savini, Vincenzo; Salutari, Prassede; Sborgia, Marco; Mancini, Iole; Masciarelli, Gioviana; Catavitello, Chiara; Astolfi, Daniela; D’Amario, Claudio; Fioritoni, Giuseppe; Spadaro, Antonio; D’Antonio, Domenico

    2011-01-01

    Rhodococcus equi is an uncommon Gram positive, variably acid-fast pathogen, that appears as hard to treat mostly owing to the establishment of intracellular niches. Lack of interpretive criteria for susceptibility testing may lead to under-reporting or overestimation of resistances, whereas knowledge about this pathogen’s clinical impact may be affected by erroneous phenotype-based characterization at a genus and species level. We present the case of a bacteraemia with a concomitant lung mass in a lymphoma patient, that further highlights the emergence of rhodococcal diseases as a matter for concern in the fields of infectious diseases and haematology. PMID:21625310

  17. Danazol: an effective option in acquired amegakaryocytic thrombocytopaenic purpura.

    PubMed

    Mulroy, E; Gleeson, S; Chiruka, S

    2015-01-01

    Acquired amegakaryocytic thrombocytopaenic purpura (AATP) is a rare haematological condition characterised by isolated thrombocytopaenia with normal other cell lines. It is often initially misdiagnosed as immune thrombocytopaenic purpura but has characteristic bone marrow findings of reduced megakaryocyte numbers. The optimal treatment of AATP is not clearly defined but revolves around immunosuppressive therapies. We report a case of successful treatment of AATP with danazol, an antioestrogenic medication. We also review the aetiologies and pathogenesis of the disorder and suggest that danazol should be considered as an effective alternative to potent immunosuppression in AATP. PMID:25945269

  18. Anaemia in systemic lupus erythematosus: from pathophysiology to clinical assessment

    PubMed Central

    Giannouli, S; Voulgarelis, M; Ziakas, P D; Tzioufas, A G

    2006-01-01

    Haematological abnormalities are common in systemic lupus erythematosus. Anaemia is found in about 50% of patients, with anaemia of chronic disease being the most common form. Impaired erythropoietin response and presence of antibodies against erythropoietin may contribute to the pathogenesis of this type of anaemia. Patients with autoimmune haemolytic anaemia usually belong to a distinct category, which is associated with anticardiolipin antibodies, thrombosis, thrombocytopenia, and renal disease, often in the context of secondary antiphospholipid syndrome. Autoantibodies, T lymphocytes, and deregulation of the cytokine network can affect bone marrow erythropoiesis, leading to anaemia. PMID:16079164

  19. Nodular regenerative hyperplasia of the liver, CREST syndrome and primary biliary cirrhosis: an overlap syndrome?

    PubMed Central

    McMahon, R F; Babbs, C; Warnes, T W

    1989-01-01

    Nodular regenerative hyperplasia of the liver (NRHL) has been found in association with collagen vascular diseases, after drug therapy, with autoimmune disease, and with a variety of haematological disorders. The association of NRHL with the syndrome of Calcinosis cutis, Raynaud's phenomenon, oesophageal dysfunction, sclerodactyly and telangiectasia (CREST syndrome) has only been reported on two previous occasions. The liver disease usually associated with CREST syndrome is primary biliary cirrhosis (PBC) and recently nodular hyperplasia of the liver has been reported in patients with early stage PBC. We present a case of NRHL with CREST syndrome and serological and biochemical features of PBC, a newly recognised overlap syndrome. Images Figure PMID:2583572

  20. Plasmodium simium/Plasmodium vivax infections in southern brown howler monkeys from the Atlantic Forest

    PubMed Central

    Costa, Daniela Camargos; da Cunha, Vanessa Pecini; de Assis, Gabriela Maria Pereira; de Souza, Júlio César; Hirano, Zelinda Maria Braga; de Arruda, Mércia Eliane; Kano, Flora Satiko; Carvalho, Luzia Helena; de Brito, Cristiana Ferreira Alves

    2014-01-01

    Blood infection by the simian parasite, Plasmodium simium, was identified in captive (n = 45, 4.4%) and in wild Alouatta clamitans monkeys (n = 20, 35%) from the Atlantic Forest of southern Brazil. A single malaria infection was symptomatic and the monkey presented clinical and haematological alterations. A high frequency of Plasmodium vivax-specific antibodies was detected among these monkeys, with 87% of the monkeys testing positive against P. vivax antigens. These findings highlight the possibility of malaria as a zoonosis in the remaining Atlantic Forest and its impact on the epidemiology of the disease. PMID:25099335

  1. Plasmodium simium/Plasmodium vivax infections in southern brown howler monkeys from the Atlantic Forest.

    PubMed

    Costa, Daniela Camargos; da Cunha, Vanessa Pecini; de Assis, Gabriela Maria Pereira; de Souza Junior, Júlio César; Hirano, Zelinda Maria Braga; de Arruda, Mércia Eliane; Kano, Flora Satiko; Carvalho, Luzia Helena; de Brito, Cristiana Ferreira Alves

    2014-08-01

    Blood infection by the simian parasite, Plasmodium simium, was identified in captive (n = 45, 4.4%) and in wild Alouatta clamitans monkeys (n = 20, 35%) from the Atlantic Forest of southern Brazil. A single malaria infection was symptomatic and the monkey presented clinical and haematological alterations. A high frequency of Plasmodium vivax-specific antibodies was detected among these monkeys, with 87% of the monkeys testing positive against P. vivax antigens. These findings highlight the possibility of malaria as a zoonosis in the remaining Atlantic Forest and its impact on the epidemiology of the disease. PMID:25099335

  2. [Cytokines. Transmittor substances of the immune system].

    PubMed

    Pisa, P; Söder, O

    1995-07-12

    Cytokines are hormone-like proteins and peptides whose principal function is that of signalling substances within the immunoinflammatory and haematopoietic systems. Since the first cytokines were characterised 15 years ago, over 50 cytokines have been strictly defined and characterised. Cytokines are classified mainly on the basis of functional criteria into families--e.g, interleukins, interferons, colony stimulating factors, and chemokines. The article provides a broad review of cytokine physiology and pathophysiology with an emphasis on recent findings of their involvement in various diseases such as infections, autoimmune and haematological disorders, and cancer. Different treatment modalities that affect cytokine activity are discussed. PMID:7637466

  3. The ninth International Conference of Anticancer Research, 6–10 October 2014, Sithonia, Greece

    PubMed Central

    Sansom, Clare E

    2014-01-01

    The ninth conference of the International Institute for Anticancer Research, held in Sithonia, Greece in October 2014, included over 700 abstracts presented in 79 separate sessions and featured a wide range of topics in basic and clinical cancer research. This report describes a small but representative sample of these sessions. It covers some recent developments in research into the basic signal transduction pathways involved in carcinogenesis; a special session on the role of homeobox genes in cancer development; and clinical sessions covering advances in breast cancer, haematological cancers, and chemotherapy. PMID:25525461

  4. Clinical presentation of coeliac disease in adult gastroenterological practice

    PubMed Central

    Boyd, S; Collins, B J; Bell, P M; Love, A H G

    1985-01-01

    Clinical features, complications and results of investigations are analysed in 50 patients diagnosed by jejunal biopsy as having coeliac disease at the Adult Gastroenterology Unit, Royal Victoria Hospital, Belfast, between 1969 and 1983. Only one patient was entirely asymptomatic, but 22% had no disturbance of bowel habit, and 50% had not lost weight. There were relatively few physical abnormalities on clinical examination. Screening tests using standard haematological and biochemical methods were positive only in between 8% and 52% of patients. More specific tests for malabsorption were positive in between 54% and 84% of patients. Jejunal biopsy remains the definitive procedure to identify patients with coeliac disease. PMID:4095802

  5. Remission of aHUS neurological damage with eculizumab

    PubMed Central

    Ávila, Ana; Vizcaíno, Belén; Molina, Pablo; Gavela, Eva; Perez-Ebri, Maria; Pallardó, Luís

    2015-01-01

    Atypical haemolytic uraemic syndrome (aHUS) is a rare disease characterized by haemolytic microangiopathic anaemia, thrombocytopaenia and acute onset of renal failure, in the absence of Escherichia coli infection. Renal damage usually progresses to end-stage renal disease (ESRD), sometimes being accompanied by signs of extrarenal thrombotic microangiopathy (TMA). We report a case of full neurological and haematological recovery after eculizumab treatment in a patient with ESRD secondary to chronic aHUS refractory to plasmatherapy while she was under dialysis. It highlights the use of eculizumab for controlling extrarenal manifestations of aHUS in this population. PMID:25815183

  6. Transfusion medicine education session.

    PubMed

    Avent, Neil D

    2009-12-01

    The European Haematology Association annual meeting is the second largest conference organized specifically for hematologists. This meeting report gives an overview of one particular session within this meeting, that of the transfusion medicine subject speciality, which included two speakers from the USA and one from the UK. The following lectures will be reviewed here: Jo-Ann Moulds--Transfusion policies and practices in alloimmunized sickle cell patients; Derwood Pamphilon--Transfusion problems in stem cell transplant patients; and Naomi Luban--Transfusion issues in neonatal and pediatric hematology. The session, therefore, included a significant discourse in clinical practice of transfusing the difficult to transfuse patient. PMID:21082955

  7. The prevention and treatment of cytomegalovirus infection in haematopoietic stem cell transplantation.

    PubMed

    Tuthill, Mark; Chen, Frederick; Paston, Samantha; De La Peńa, Hugo; Rusakiewicz, Sylvie; Madrigal, Alejandro

    2009-09-01

    Allogeneic haematopoietic stem cell transplantation (HSCT) is an intensive medical treatment involving myeloablative chemo-radiotherapy followed by stem cell rescue using allogeneic haematopoietic stem cells harvested from HLA-matched donors, which is primarily used for the treatment of haematological malignancies. Cytomegalovirus (CMV) infection is one of the major causes of morbidity and death after HSCT. This focused research review highlights the advances made with research into CMV in the HSCT setting. It provides the reader with an overview of current CMV research into the prevention and management of CMV infection. PMID:19466407

  8. Moynihan Lecture, 1980. Major surgery in haemophilia.

    PubMed Central

    Rudowski, W. J.

    1981-01-01

    The experience of the Department of Surgery of the Institute of Haematology, Warsaw, in surgery in haemophilic patients is discussed. From 1961 to 1980 131 surgical procedures were carried out on 110 patients with a mortality of 4.5%. The principles of replacement therapy, advantages of factor VIII concentrates, and factors influencing the haemorrhagic complications are described. The management of patients with inhibitors of factor VIII and the effect of the 'Feiba' preparation is discussed. Finally the importance of new work on an antihaemophilic globulin-liposome compound for oral administration to haemophilic patients is emphasised. PMID:6787973

  9. Treatment-related deaths in second complete remission in childhood acute myeloid leukaemia.

    PubMed

    Molgaard-Hansen, Lene; Möttönen, Merja; Glosli, Heidi; Jónmundsson, Guđmundur K; Abrahamsson, Jonas; Hasle, Henrik

    2011-03-01

    The frequency and causes of treatment-related deaths (TRD) in second complete remission (CR2) in acute myeloid leukaemia (AML) were investigated in a historical, prospective cohort study of 429 children included in the Nordic Society of Paediatric Haematology and Oncology (NOPHO)-AML-88 and -93 trials. Relapse occurred in 158 children (39%). Seventeen (18%) of the 96 patients entering CR2 suffered TRD. The main causes were infection (59%) and complications from graft-versus-host disease (22%). Fourteen (82%) of 17 TRDs occurred in children undergoing haematopoietic stem cell transplantations (HSCT). Optimal supportive care after HSCT is essential, and studies on risk factors for TRD are needed. PMID:21241281

  10. Influence of gastrointestinal trichostrongylidosis on ram fertility.

    PubMed

    Gaglio, G; Poglayen, G; Capelli, G; Gruner, L; Maras, L; Giannetto, S; Scala, A

    2010-01-01

    A study on the influence of gastrointestinal trichostrongyles on ram fertility was performed. Two groups of semen donor rams (Sarda breed) were utilized; one was experimentally infected with Haemonchus contortus, Trichostrongylus colubriformis and Teladorsagia circumcincta and the other was used as a control group. In all animals, coprological, haematological and reproductive parameters were studied. The results suggest that the parasites had a limited effect with some changes in phosphorus, cholesterol and chlorine levels. In our experience the parasitic burden produced no significant change on ram fertility. PMID:21370756

  11. Rituximab-associated interstitial lung disease in fibrillary glomerulonephritis

    PubMed Central

    Sainz-Prestel, Valeria; Hernandez-Perez, Jesus; Rojas-Rivera, Jorge; Milicua-Muńoz, José María; Egido, Jesus; Ortiz, Alberto

    2013-01-01

    Rituximab (RTX) is a chimeric monoclonal antibody against CD20+ B cells increasingly used to treat kidney disorders. RTX-induced pulmonary disease has been reported in patients treated for haematological disorders, and a few cases have been observed in patients with underlying rheumatological conditions. We report a case of non-infectious interstitial pneumonitis associated with RTX use in a 49-year-old patient with primary (fibrillary) glomerulonephritis. As typically observed, discontinuation of the drug and prompt initiation of glucocorticoids led to resolution of pulmonary manifestations. However, fatalities have been reported and nephrologists treating glomerulonephritis patients with RTX should be aware of the existence of this potentially lethal complication.

  12. Outbreak of botulism in a dairy herd in Turkey

    PubMed Central

    2007-01-01

    In this study, the clinical findings and results of haematological and biochemical analyses of 26 cattle with botulism were evaluated. The most important clinical signs in the affected cattle included: decreased appetite, ataxia, difficulty to rise, loss of tongue tone, salivation and bradycardia. A definitive diagnosis of botulism was based on demonstration of the preformed toxin in ruminal and intestinal contents and feed materials including poultry litter, by mouse inoculation test. This study is the first confirmation, by direct toxin isolation, of Clostridium botulinum type C and Clostridium botulinum type D in cattle, in Turkey. PMID:21851698

  13. Antiviral treatment including entecavir plus tenofovir disoproxil fumarate for HBV reactivation following a rituximab-based regimen.

    PubMed

    Rago, Angela; Lichtner, Miriam; Mecarocci, Sergio; Marocco, Raffaella; Cenfra, Natalia; Belvisi, Valeria; Del Borgo, Cosmo; Cimino, Giuseppe; Mastroianni, Claudio M

    2010-01-01

    Entecavir and tenofovir disoproxil fumarate are potent and effective antiviral drugs that now represent recommended treatment options for chronic HBV infection. However, no or very limited clinical evidence is currently available on these drugs for the management of HBV reactivation in patients with haematological malignancies. Herein, we report a case of HBV reactivation in a patient with non-Hodgkin's lymphoma following a rituximab-based regimen, and who was successfully treated with a combination antiviral treatment including entecavir and tenofovir disoproxil fumarate. PMID:20834107

  14. Paralysis in hedgehogs (Erinaceus europaeus) associated with demyelination.

    PubMed

    Palmer, A C; Blakemore, W F; Franklin, R J; Frost, L M; Gough, R E; Lewis, J C; Macdougall, D F; O'Leary, M T; Stocker, L R

    1998-11-14

    Paraplegia affected 14 hedgehogs (Erinaceus europaeus) in a wildlife rescue hospital over a period of six months. Postmortem examination revealed demyelination in the brain and spinal cord and an inflammatory response in the meninges, choroid plexus and CNS. The peripheral nervous system was not affected. In the spleen, lungs and liver there was an accumulation of megakaryocytes and other evidence of extramedullary haemopoiesis, but there was no haematological evidence of anaemia. The pattern of disease incidence and the nature of the changes in the CNS suggest they were of viral origin, but no causal agent was isolated and the possibility of a neurotoxin cause cannot be ruled out. PMID:9854316

  15. A cross sectional study of employees with potential occupational exposure to ethylene oxide.

    PubMed Central

    Currier, M F; Carlo, G L; Poston, P L; Ledford, W E

    1984-01-01

    A cross sectional study was conducted on 84 employees potentially exposed to ethylene oxide (EO) to determine whether they had a higher prevalence of abnormalities of the haematopoietic, hepatic, or renal systems than a control group individually matched on age, hire date, race, smoking habits, alcohol history, and date of examination. Potential exposures were estimated to be generally below the current threshold limit value of 10 ppm, with most below 1 ppm. Results of the haematological and biochemical studies were similar in the two groups. A statistically significant (p = 0.035) increase in the prevalence of proteinuria was noted among the EO group which warrants further investigation. PMID:6333892

  16. [Reference values for biological parameters in occupational and environmental medicine].

    PubMed

    Xue, H; Huang, J

    1997-03-01

    Biological parameters are extensively used in occupational and environmental medicine. They are traditionally evaluated by comparison with normal range. Since 1970's, with the growing skepticism about normal range and researches' progress, the replacing notion of reference values and related theory had gradually come into being with the one which is the most representative, published by International Federation of Clinical Chemistry and International Committee for Standardization in Haematology. This paper introduces those results with emphatic discussion on data processing and statistical procedures. The process of establishing reference limits is illustrated by an example and issues on reference and decision limits, reference band, multivariate reference values are also briefly addressed. PMID:10325620

  17. Prospective study of aetiological factors in burning mouth syndrome

    PubMed Central

    Lamey, P-J; Lamb, A B

    1988-01-01

    A prospective study of 150 consecutive patients with burning mouth syndrome and with a minimum follow up period of 18 months is reported. Factors related to dentures, to vitamin B complex deficiency, and to psychological abnormalities were found to be important, and undiagnosed diabetes mellitus, reduced salivary gland function, haematological deficiencies, candidal infection, parafunctional habits, and allergy might also play a part. Given a protocol for management which takes all these factors into account, some two thirds of patients can be cured or have their symptoms improved. PMID:3133028

  18. Danazol: An Effective Option in Acquired Amegakaryocytic Thrombocytopaenic Purpura

    PubMed Central

    Mulroy, E.; Gleeson, S.; Chiruka, S.

    2015-01-01

    Acquired amegakaryocytic thrombocytopaenic purpura (AATP) is a rare haematological condition characterised by isolated thrombocytopaenia with normal other cell lines. It is often initially misdiagnosed as immune thrombocytopaenic purpura but has characteristic bone marrow findings of reduced megakaryocyte numbers. The optimal treatment of AATP is not clearly defined but revolves around immunosuppressive therapies. We report a case of successful treatment of AATP with danazol, an antioestrogenic medication. We also review the aetiologies and pathogenesis of the disorder and suggest that danazol should be considered as an effective alternative to potent immunosuppression in AATP. PMID:25945269

  19. Invasive Myceliophthora thermophila infection mimicking invasive aspergillosis in a neutropenic patient: a new cause of cross-reactivity with the Aspergillus galactomannan serum antigen assay.

    PubMed

    Morio, Florent; Fraissinet, François; Gastinne, Thomas; Le Pape, Patrice; Delaunay, Jacques; Sigler, Lynne; Gibas, Connie Fe C; Miegeville, Michel

    2011-11-01

    Myceliophthora thermophila is a thermophilic mould widely found in the environment but rarely responsible for human infections. We describe a case of invasive Myceliophthora thermophila infection mimicking invasive aspergillosis in a neutropenic patient with haematological malignancy. Cross-reactivity with Aspergillus galactomannan assay (GM) was demonstrated by repeated positive results and confirmed by cross-reaction between the fungal isolate and the GM assay. The patient was successfully treated with voriconazole. Potential GM cross-reactivity must be considered in future studies including patients categorized as having probable invasive aspergillosis using the GM as the only mycological criterion. PMID:21619496

  20. Quality of Life and Supportive Care in Multiple Myeloma

    PubMed Central

    Cömert, Melda; Güne?, Ajda Ersoy; ?ahin, Fahri; Saydam, Güray

    2013-01-01

    Multiple myeloma is the second most common haematological malignancy. Novel therapies have led to improvement in survival. Current myeloma management is matching the progress made in improved survival through disease control while optimising quality of life with effective supportive care. Supportive treatment is an essential part of the therapeutic management of myeloma patients because it is directed towards improving the patient’s quality of life and also can improve survival. The aim of this review is to highlight the relationship among life of quality, supportive care, and improvement in survival. Conflict of interest:None declared. PMID:24385802

  1. Bone marrow aspiration in resource poor environment: our experience in Kenya.

    PubMed

    Busarla, Satya Vara Prasad

    2015-06-01

    Bone marrow aspiration examination is performed mainly to evaluate haematological disorders. Several bone marrow aspiration needles are available that include the Salah, Klima, Jamshidi and Islam. However, cost is an issue in our local environment as most of our patients are not medically insured. We describe our experience of bone marrow aspiration using an 18-gauge lumbar puncture needle at the posterior superior iliac spine. The technique is safe and cost effective and the site is easily accessible, even in obese patients. The crush preparations provide good morphology, therefore avoiding repeats. Additional training is not required for the procedural technique. We recommend this technique for use in resource challenged settings. PMID:25823365

  2. Clinical pharmacology in normal volunteers of praziquantel, a new drug against schistosomes and cestodes

    Microsoft Academic Search

    G. Leopold; W. Ungethiim; E. Groll; H. W. Diekmann; H. Nowak; D. H. G. Wegner

    1978-01-01

    The tolerance of Praziquantel (2-cyclohexylcarbonyl-1, 3, 4, 6, 7, 11b-hexahydro-2H-pyrazino-[2, 1-a]isoquinoline-4-one) in oral doses of 1×20 mg\\/kg, 1×50 mg\\/kg, 3×10 mg\\/kg and 3×25 mg\\/kg body weight (t=4 h) was tested in a complex study involving 36 healthy volunteers. In addition to the usual assessment of clinical chemistry, haematology, coagulation physiology, urinalysis, clinico-physiological examination including EEG, and medical examination, clinico-psychological parameters

  3. Protective effects of fenugreek (Trigonella foenum graecum L.) upon dieldrin-induced toxicity in male rat.

    PubMed

    Hfaiedh, Najla; Alimi, Hichem; Murat, Jean-Claude; Elfeki, Abdelfattah

    2012-12-01

    The purpose of this study was to evaluate the protective effects of fenugreek (Trigonella foenum graecum L.) upon dieldrin-induced perturbations of haematological parameters and damages to liver and kidney of male Wistar rats. Under our experimental conditions, dieldrin poisoning resulted in 1) an alteration of several haematological parameters, 2) an oxidative stress evidenced by an increase of lipids peroxidation level associated with an increase of superoxide dismutase activity and a decrease of glutathione peroxidase and catalase activities in hepatic and renal tissues, 3) increased levels of glucose, total cholesterol, triglycerides, creatinine, urea, uric acid and proteins in blood, 4) increased activities of lactate dehydrogenase, alkaline phosphatase and transaminases in blood. Previous administration of fenugreek was found to hinder these dieldrin-induced damages: all hematological, renal and hepatic biomarkers, level of lipids peroxidation and activities of catalase and glutathione-peroxidase in liver and kidney were kept close to control values. This protective effect is mainly attributed to antioxidant properties of fenugreek. PMID:23255669

  4. Gemcitabine-oxaliplatin combination for ovarian cancer resistant to taxane-platinum treatment: a phase II study from the GINECO group

    PubMed Central

    Ray-Coquard, I; Weber, B; Cretin, J; Haddad-Guichard, Z; Lévy, E; Hardy-Bessard, A C; Gouttebel, M C; Geay, J-F; Aleba, A; Orfeuvre, H; Agostini, C; Provencal, J; Ferrero, J M; Fric, D; Dohollou, N; Paraiso, D; Salvat, J; Pujade-Lauraine, É

    2009-01-01

    Advanced ovarian carcinoma in early progression (<6 months) (AOCEP) is considered resistant to most cytotoxic drugs. Gemcitabine (GE) and oxaliplatin (OXA) have shown single-agent activity in relapsed ovarian cancer. Their combination was tested in patients with AOCEP in phase II study. Fifty patients pre-treated with platinum–taxane received q3w administration of OXA (100?mg?m–2, d1) and GE (1000?mg?m–2, d1, d8, 100-min infusion). Patient characteristics were a : median age 64 years (range 46–79),and 1 (84%) or 2 (16%) earlier lines of treatment. Haematological toxicity included grade 3–4 neutropaenia (33%), anaemia (8%), and thrombocytopaenia (19%). Febrile neutropaenia occurred in 3%. Non-haematological toxicity included grade 2–3 nausea or vomiting (34%), grade 3 fatigue (25%),and grade 2 alopecia (24%). Eighteen (37%) patients experienced response. Median progression-free (PF) and overall survivals (OS) were 4.6 and 11.4 months, respectively. The OXA–GE combination has high activity and acceptable toxicity in AOCEP patients. A comparison of the doublet OXA–GE with single-agent treatment is warranted. PMID:19190632

  5. Acquired amegakaryocytic thrombocytopenic purpura associated with immunoglobulin deficiency.

    PubMed

    Rovira, M; Feliu, E; Florensa, L; Woessner, S; Tassies, D; Montserrat, E; Vives-Corrons, J L; Rozman, C

    1991-01-01

    Acquired amegakaryocytic thrombocytopenic purpura (AATP) is a haematological disorder characterized by severe thrombocytopenia due to an immunologically induced absence of megakaryocytes in an otherwise normal-appearing bone marrow. A 57-year-old male with a 6-month history of rectal and cutaneous bleeding is reported. Platelet count was 10 X 10(9)/l, while other haematological values were within the normal range, except for the presence of hypogammaglobulinaemia with decreased IgA and IgG. Both platelet median volume and half-life span were normal, and antiplatelet IgG determinations were negative. Bone marrow aspiration and biopsy showed no megakaryocytes, with a normal appearance of erythroblastic and granulopoietic series. An in vitro culture for megakaryocytic progenitor cells did not show any growth of megakaryocyte colonies. No inhibitory effect on the growth of normal marrow megakaryocytic colonies was observed when serum and lymphocytes of the patient were added. Following 4 weeks of prednisone therapy, the platelet count rose to 127 X 10(9)/l and the bone marrow aspirate showed some megakaryocytes. The possible pathogenetic mechanisms of this entity are discussed. PMID:2011928

  6. Toxic effects of malathion in carp, Cyprinus carpio carpio: protective role of lycopene.

    PubMed

    Yonar, Serpil Mi?e

    2013-11-01

    The present study was carried out in order to investigate the potential protective effects of lycopene against malathion-induced toxicity in carp. The fish were exposed to sublethal concentrations of malathion (0.5 and 1mgL(-1)) for 14 days, and lycopene (10mgkg(-1) of fish weight) was simultaneously administered. Samples of the blood and tissue (liver, kidneys, and gills) were collected at the end of the experimental period and their haematological profiles [red blood cell (RBC) counts, haemoglobin (Hb) concentrations, haematocrit (Ht) levels, and erythrocyte indices, including the mean corpuscular volume (MCV), the mean corpuscular haemoglobin (MCH) and the mean corpuscular haemoglobin concentration (MCHC)], immune responses [white blood cell (WBC) counts, oxidative radical production (nitroblue tetrazolium (NBT) activity), total plasma protein (TP) and total immunoglobulin (TI) levels and phagocytic activities (PA)] and oxidant/antioxidant statuses [malondialdehyde (MDA) levels, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities, and reduced glutathione (GSH) concentrations] were analysed. The findings of the present study demonstrated that the exposure of carp to malathion resulted in alterations in the haematological profiles and immune responses, and lead to increased reactive oxygen species formation, resulting in oxidative damage and inhibition of the antioxidant capacities. However, the administration of lycopene prevented malathion-induced toxic effects. PMID:23932509

  7. Temozolomide and Radiotherapy versus Radiotherapy Alone in High Grade Gliomas: A Very Long Term Comparative Study and Literature Review

    PubMed Central

    Parisi, Salvatore; Corsa, Pietro; Raguso, Arcangela; Perrone, Antonio; Cossa, Sabrina; Munafň, Tindara; Sanpaolo, Gerardo; Donno, Elisa; Clemente, Maria Antonietta; Piombino, Michele; Parisi, Federico; Valle, Guido

    2015-01-01

    Temozolomide (TMZ) is the first line drug in the care of high grade gliomas. The combined treatment of TMZ plus radiotherapy is more effective in the care of brain gliomas then radiotherapy alone. Aim of this report is a survival comparison, on a long time (>10 years) span, of glioma patients treated with radiotherapy alone and with radiotherapy + TMZ. Materials and Methods. In this report we retrospectively reviewed the outcome of 128 consecutive pts with diagnosis of high grade gliomas referred to our institutions from April 1994 to November 2001. The first 64?pts were treated with RT alone and the other 64 with a combination of RT and adjuvant or concomitant TMZ. Results. Grade 3 (G3) haematological toxicity was recorded in 6 (9%) of 64?pts treated with RT and TMZ. No G4 haematological toxicity was observed. Age, histology, and administration of TMZ were statistically significant prognostic factors associated with 2 years overall survival (OS). PFS was for GBM 9 months, for AA 11. Conclusions. The combination of RT and TMZ improves long term survival in glioma patients. Our results confirm the superiority of the combination on a long time basis. PMID:25815327

  8. Reversible antifertility effect of aqueous leaf extract of Allamanda cathartica L. in male laboratory mice.

    PubMed

    Singh, A; Singh, S K

    2008-12-01

    The efficacy of oral administration of aqueous leaf extract of Allamanda cathartica (150 mg kg(-1) body weight day(-1) for 14, 28 and 42 days) in inducing infertility and changes in various male reproductive endpoints was evaluated in Parkes strain mice. The effect of the treatment on organ weight, histopathology, sperm parameters, testosterone level, haematology, serum biochemistry and on fertility indices was assessed. Histologically, testes in extract-treated mice showed nonuniform degenerative changes in the seminiferous tubules as both affected and normal tubules were observed in the same sections. The treatment also had adverse effects on motility, viability, morphology and on number of spermatozoa in the cauda epididymidis. Serum levels of testosterone, alanine aminotransferase, aspartate aminotransferase and creatinine, haematological parameters and liver and kidney histoarchitecture were, however, not affected by the treatment. Fertility of the extract-treated males was also suppressed, although the libido remained unaffected. By 56 days of treatment withdrawal, however, the above parameters recovered to control levels. Our results thus suggest that A. cathartica treatment causes reversible suppression of fertility in male mice, without causing detectable toxic effects. PMID:19032682

  9. Mild haemorheological changes induced by a moderate endurance exercise in patients with sickle cell anaemia.

    PubMed

    Balayssac-Siransy, Edwige; Connes, Philippe; Tuo, Nalourgo; Danho, Clotaire; Diaw, Mor; Sanogo, Ibrahima; Hardy-Dessources, Marie-Dominique; Samb, Abdoulaye; Ballas, Samir K; Bogui, Pascal

    2011-08-01

    The levels and duration of physical activity that can be considered as completely safe in patients with sickle cell anaemia (SCA) is unknown. The present study compared the haemorheological and haematological profile, cell density distribution and basic biochemistry between a group of 17 patients with SCA and 21 healthy subjects before and after a 20 min duration submaximal cycling exercise at the same absolute workload. Blood was sampled at rest and 3 min after the end of exercise for measurement of biological parameters. Exercise did not affect the haematocrit and blood viscosity in the two groups. Plasma viscosity was not different between the two groups at rest and similarly increased with exercise. The proportion of intermediary dense cells (with density between 1·11 and 1·12 g/ml) decreased with exercise in the SCA group resulting in an increase in the proportion of red blood cells with a density >1·12 g/ml. No change was observed in the control group. The present study suggests that mild-moderate exercise is not very harmful for SCA patients. The haemorheological and haematological changes were very mild, except for the formation of dense cells but no clinically significant signs of medical complication were present in any of the patients. PMID:21569006

  10. Current markers of the Athlete Blood Passport do not flag microdose EPO doping.

    PubMed

    Ashenden, Michael; Gough, Clare E; Garnham, Andrew; Gore, Christopher J; Sharpe, Ken

    2011-09-01

    The Athlete Blood Passport is the most recent tool adopted by anti-doping authorities to detect athletes using performance-enhancing drugs such as recombinant human erythropoietin (rhEPO). This strategy relies on detecting abnormal variations in haematological variables caused by doping, against a background of biological and analytical variability. Ten subjects were given twice weekly intravenous injections of rhEPO for up to 12 weeks. Full blood counts were measured using a Sysmex XE-2100 automated haematology analyser, and total haemoglobin mass via a carbon monoxide rebreathing test. The sensitivity of the passport to flag abnormal deviations in blood values was evaluated using dedicated Athlete Blood Passport software. Our treatment regimen elicited a 10% increase in total haemoglobin mass equivalent to approximately two bags of reinfused blood. The passport software did not flag any subjects as being suspicious of doping whilst they were receiving rhEPO. We conclude that it is possible for athletes to use rhEPO without eliciting abnormal changes in the blood variables currently monitored by the Athlete Blood Passport. PMID:21336951

  11. Haematobiochemical parameters of goats fed tannin rich Psidium guajava and Carissa spinarum against Haemonchus contortus infection in India.

    PubMed

    Jan, Owais Qadir; Kamili, Neyaz; Ashraf, Ajmal; Iqbal, Asif; Sharma, R K; Rastogi, Ankur

    2015-03-01

    The antihelminthic properties of tannin-rich plants are being explored as an alternative to chemical drugs to minimise the effects of gastro intestinal nematodes (GIN). The present study was, therefore, conducted to investigate the effect of condensed tannins (CT), obtained from regional tanniferous tree leaves, in the Haemonchus contortus infected goats on the heamatobiochemical parameters to assess the goat health. Twelve adult male goats were randomly divided into three equal groups, namely negative control, infected control and treatment. H. contortus infected goats were allocated into infected control and treatment groups and their feeds contained 0 and 1.96 % of CT, respectively. Feeding trial was conducted for the duration of 90 days during which haematological and serum biochemical parameters were monitored on fortnightly basis. The animals ingesting the CT-rich leaf meal mixture had increased levels of haemoglobin, packed cell volume, total protein, globulin, glucose and calcium, and decreased levels of blood urea; indicating a beneficial effect of CT supplementation at the selected level. However, the phosphorus balance, serum albumin levels and serum enzyme activity were not affected significantly. The study revealed that inclusion of CT in the diets of the adult male goats did not pose any threat to the health of the goats. Further, the CT based diet had beneficial impact on the haematological parameters and could therefore be included in small ruminant diets to minimize the impact of GIN. PMID:25698858

  12. Experimental mycotoxic nephropathy in pigs provoked by a diet containing ochratoxin A and penicillic acid.

    PubMed

    Stoev, S D; Vitanov, S; Anguelov, G; Petkova-Bocharova, T; Creppy, E E

    2001-04-01

    Mycotoxic nephropathy was induced in 18 young pigs by diets contaminated with strains of Aspergillus ochraceus containing ochratoxin A (OTA) and penicillic acid (PA) at levels corresponding to those naturally encountered in animal feeds in Bulgaria. Haematological and biochemical parameters, as well as the morphological and ultrastructural changes in various internal organs, and especially in the kidneys, were examined at different stages of development of the disease. A mottled surface of the kidneys was only seen in pigs exposed to a mouldy diet containing 180 ppb OTA for 3 months, but microscopic lesions, as well as changes in various haematological and biochemical parameters, were observed in all groups exposed to the same mouldy diet containing only 90 or 180 ppb OTA. Histological examination showed two types of change: degenerative changes affecting the epithelial cells of the proximal tubules, which predominated at the initial stage, and proliferative changes in the interstitium, which predominated at the later stage of the disease. Telangiectasis and lymph stasis were also seen, as well as degenerative changes in the capillary endothelium. The characteristic renal lesions were similar to those observed in spontaneous cases of mycotoxic porcine nephropathy in Bulgaria, but they were a little different from the classic Danish porcine nephropathy. The enhanced toxicity of OTA in our study may be due to a synergistic effect between OTA and PA or to some other unknown metabolites produced by the same ochratoxinogenic strains of A. ochraceus. PMID:11334150

  13. Influence of ochratoxin A and an extract of artichoke on the vaccinal immunity and health in broiler chicks.

    PubMed

    Stoev, S D; Anguelov, G; Ivanov, I; Pavlov, D

    2000-03-01

    The combined effect of ochratoxin A (at diet levels of 130, 305 and 790 ppb) and penicillic acid was studied in 100 broiler chicks. Serological investigations revealed significantly lower haemagglutination inhibiting antibody titers in the experimental chicks immunized with vaccine against Newcastle disease. A statistically significant decrease of the body weight and the relative weight of lymphoid organs as well as a significant increase of the relative weight of kidneys and liver were seen. The main degenerative changes were observed in the proximal convoluted tubules in kidneys and slight degenerative changes were found in the hepatocytes. Degenerative changes and depletion of lymphoid cells were observed in the bursa Fabricii, thymus, spleen and Peyer's patches of intestinal mucosa. Serum analyses revealed significant decreases of the total protein and cholesterol, and significant increases of the uric acid and glucose. Haematological analyses showed a slight anaemia, leucocytosis and slightly decompensated metabolic acidosis. A statistically significant protective effect of 5% total water extract of artichoke on humoral immune response (increase of haemaglutination inhibiting antibody titer), relative organ weight as well as on pathomorphological, haematological and biochemical changes induced by ochratoxin A, was established. PMID:10779152

  14. Low frequency of anti-D alloimmunization following D+ platelet transfusion: the Anti-D Alloimmunization after D-incompatible Platelet Transfusions (ADAPT) study.

    PubMed

    Cid, Joan; Lozano, Miguel; Ziman, Alyssa; West, Kamille A; O'Brien, Kerry L; Murphy, Michael F; Wendel, Silvano; Vázquez, Alejandro; Ortín, Xavier; Hervig, Tor A; Delaney, Meghan; Flegel, Willy A; Yazer, Mark H

    2015-02-01

    The reported frequency of D alloimmunization in D- recipients after transfusion of D+ platelets varies. This study was designed to determine the frequency of D alloimmunization, previously reported to be an average of 5 ± 2%. A primary anti-D immune response was defined as the detection of anti-D ? 28 d following the first D+ platelet transfusion. Data were collected on 485 D- recipients of D+ platelets in 11 centres between 2010 and 2012. Their median age was 60 (range 2-100) years. Diagnoses included: haematological (203/485, 42%), oncological (64/485, 13%) and other diseases (218/485, 45%). Only 7/485 (1·44%; 95% CI 0·58-2·97%) recipients had a primary anti-D response after a median serological follow-up of 77 d (range: 28-2111). There were no statistically significant differences between the primary anti-D formers and the other patients, in terms of gender, age, receipt of immunosuppressive therapy, proportion of patients with haematological/oncological diseases, transfusion of whole blood-derived or apheresis platelets or both, and total number of transfused platelet products. This is the largest study with the longest follow-up of D alloimmunization following D+ platelet transfusion. The low frequency of D alloimmunization should be considered when deciding whether to administer Rh Immune Globulin to D- males and D- females without childbearing potential after transfusion of D+ platelets. PMID:25283094

  15. Initial effects of the grounding of the tanker Braer on health in Shetland. The Shetland Health Study Group.

    PubMed Central

    Campbell, D; Cox, D; Crum, J; Foster, K; Christie, P; Brewster, D

    1993-01-01

    OBJECTIVE--To determine if the oil spillage from the tanker Braer had any immediate health effects on the exposed resident population. DESIGN--Cohort study with a comparison against controls, exposure status being assigned on the basis of geographical location. SETTING--Rural Shetland. SUBJECTS--All those resident on or after 5 January 1993 (day 0) within 4.5 km of the site of tanker's grounding. Controls matched for sex and age were drawn from a general practice list 95 km distant. OUTCOME MEASURES--Demographic details; smoking and alcohol consumption; perception of health and reported presence or absence of specific symptoms; peak expiratory flow; results of haematology, liver and renal function tests, and blood and urine toxicology. RESULTS--Of subjects contacted, 420 (66%) exposed people and 92 (68%) controls were studied; 56 non-attenders were surveyed. Principal health effects arose on days 1 and 2 and were headache, throat irritation, and itchy eyes. No significant differences between those exposed and controls were found for any of the biological markers. Toxicological studies did not show any exposures that are known to affect human health. CONCLUSIONS--The study confirmed the anecdotal reports of certain acute symptoms. No evidence of pulmonary, haematological, renal, or hepatic damage was detected at the population level. Toxicological samples from exposed people did not find levels known to affect human health. Further studies are required to ascertain whether there have been any long term effects on the population. PMID:8281057

  16. Bendamustine in combination with thalidomide and dexamethasone is a viable salvage option in myeloma relapsed and/or refractory to bortezomib and lenalidomide.

    PubMed

    Lau, I-Jun; Smith, Dean; Aitchison, Robin; Blesing, Norbert; Roberts, Pamela; Peniket, Andrew; Yong, Kwee; Rabin, Neil; Ramasamy, Karthik

    2015-04-01

    Treatment options are limited in myeloma relapsed or refractory to both bortezomib and lenalidomide (double-relapsed/refractory multiple myeloma; DRMM). Bendamustine is an antitumour agent that has efficacy in relapsed myeloma. We retrospectively analysed data from 30 DRMM patients who received a combination of bendamustine, thalidomide and dexamethasone (BTD) in 28-day treatment cycles. Bendamustine was administered with a cumulative dose of up to 200 mg/m(2). Thalidomide (50-150 mg) was given daily as tolerated, and dexamethasone was given at an equivalent dose of up to 160 mg per cycle. A median of 5 (2-9) treatment cycles were administered per patient. Twenty-six patients (87 %) achieved stable disease or better. At a median follow-up time of 12.1 (2.3-21.5) months, median (95 % CI) progression-free survival and overall survival were 4.0 (2.6-5.3) months and 7.2 (5.2-9.2) months, respectively. The most common grade 3-4 adverse events were haematological: anaemia (n?=?8, 34.8 %), neutropenia (n?=?16, 69.6 %) and thrombocytopenia (n?=?10, 43.5 %). Non-haematological toxicities included pain (n?=?3, 13.0 %), infection (n?=?7, 30.4 %) and sensory neuropathy (n?=?1, 4.3 %). We propose that BTD is a viable salvage treatment option for DRMM patients. PMID:25345871

  17. Tanespimycin monotherapy in relapsed multiple myeloma: results of a phase 1 dose-escalation study.

    PubMed

    Richardson, Paul G; Chanan-Khan, Asher A; Alsina, Melissa; Albitar, Maher; Berman, David; Messina, Marianne; Mitsiades, Constantine S; Anderson, Kenneth C

    2010-08-01

    Tanespimycin, a heat shock protein 90 (HSP90) inhibitor, induces apoptosis in drug-sensitive and -resistant MM cell lines and in tumour cells from patients with relapsed MM. In this phase 1 dose-escalation study, the safety, plasma pharmacokinetics, and biological/antitumour activity of tanespimycin were evaluated in heavily pretreated patients with relapsed/refractory MM. Tanespimycin (150-525 mg/m(2)) was given on days 1, 4, 8, and 11 of each 3-week cycle for up to 8 cycles. Non-haematological AEs included diarrhoea (59%), back pain (35%), fatigue (38%), and nausea (35%); haematological AEs included anaemia (24%) and thrombocytopenia (21%). One patient (3%) achieved minimal response (MR), with a progression-free survival (PFS) of 3 months, a 41% decrease from baseline in urine M protein, and a 33% decrease from baseline in serum M protein. Fifteen patients (52%) achieved SD with a median PFS of 2.1 months; 5/15 had reductions in serum M protein ranging from 7% to 38% and in urine M protein ranging from 6% to 91%. Mean HSP70 levels increased from day 1 h 0 to day 1 h 4 with further increases on day 11 h 0 and day 11 h 4, consistent with a therapeutic treatment effect. Tanespimycin monotherapy was well tolerated and demonstrated activity across all doses tested. PMID:20618337

  18. Blood film review by biomedical scientists.

    PubMed

    Sinclair, G J

    2005-01-01

    This study aims to provide evidence of biomedical scientists' competence in blood film cytomorphology and to improve continuing assessment and training. Twenty photomicrographs are prepared from historical teaching slides, and a slide projector timer is designed and built to trigger slide advance automatically every 30 sec. Haematology staff in local district general hospitals take part in the study. During each visit, the test is explained and participants mark their observations on individual results sheets. Two sets (accepted and rejected) of observations are generated, which are validated by peer review. The accepted set is scored. No evidence is found to prove that members of each department were drawn from separate groups (P=0.36). Seniors and routine cytomorphologists are compared for each department and a non-zero difference in score was found (P=0.00). A comparison is made between the number of times a scored or non-scored observation is recorded, resulting in a probability that approaches zero. Thus, it is highly unlikely that the observations are drawn from one pool, and the test score and ability in cytomorphology would appear to be directly related. This study challenges the notion that unrestricted time, clinical data and a microscope are required for haematology blood film cytomorphology tests. Introduction of this type of test would provide biomedical science with a valuable assessment tool. PMID:15997881

  19. Dental Treatment in Patients with Leukemia

    PubMed Central

    Meurer, Maria Inęs; Grando, Liliane Janete; Gonzaga Del Moral, Joanita Ângela; da Silva Rath, Inęs Beatriz; Schaefer Tavares, Silvia

    2015-01-01

    Dental treatment of patients with leukemia should be planned on the basis of antineoplastic therapy which can be chemotherapy with or without radiotherapy and bone marrow transplantation. Many are the oral manifestations presented by these patients, arising from leukemia and/or treatment. In addition, performing dental procedures at different stages of treatment (before, during, or after) must follow certain protocols in relation to the haematological indices of patients, aimed at maintaining health and contributing to the effectiveness of the results of antineoplastic therapy. Through a literature review, the purpose of this study was to report the hematological abnormalities present in patients with leukemia, trying to correlate them with the feasibility of dental treatment at different stages of the disease. It is concluded in this paper that dental treatment in relation to haematological indices presented by patients with leukemia must follow certain protocols, mainly related to neutrophil and platelet counts, and the presence of the dentist in a multidisciplinary team is required for the health care of this patient. PMID:25784937

  20. Oral toxicity evaluation of kefir-isolated Lactobacillus kefiranofaciens M1 in Sprague-Dawley rats.

    PubMed

    Owaga, E E; Chen, M J; Chen, W Y; Chen, C W; Hsieh, R H

    2014-08-01

    Lactobacilli kefiranofaciens M1 has shown novel immunomodulation and anti-allergy probiotic attributes in cell and animal models. An acute oral toxicity assessment of L. kefiranofaciens M1 was evaluated in Sprague-Dawley rats. The rats were randomly assigned to four groups (12 rats/sex/group): the low dose group was orally gavaged with L. kefiranofaciens M1 at 3.0×10(8)cfu/kg bw while the medium dose and high dose groups received 9.0×10(9)cfu/kg bw and 1.8×10(10)cfu/kg bw, respectively, for 28days. The control group received phosphate buffer saline. The body weights were measured weekly while blood samples were collected for haematology and serum biochemistry tests. Histopathology of the organs (heart, liver, kidney, adrenal glands, spleen, ovary, testis), and urinalysis were conducted on study termination. The body weight gain of the L. kefiranofaciens M1 and control groups were comparable during the administration period. Overall, L. kefiranofaciens M1 did not induce adverse effects on haematology, serum biochemistry, and urinalysis parameters. Gross and microscopic histopathology of the organs revealed no toxicity effect of L. kefiranofaciens M1. In conclusion, 1.8×10(10)cfu/kg bw of L. kefiranofaciens M1 was considered as the no-observed-adverse-effect-level (NOAEL), which was the highest dose tested in the present study. PMID:24842838

  1. Phase I clinical trial of oral rigosertib in patients with myelodysplastic syndromes.

    PubMed

    Komrokji, Rami S; Raza, Azra; Lancet, Jeffrey E; Ren, Chen; Taft, David; Maniar, Manoj; Wilhelm, Francois; List, Alan F

    2013-08-01

    The multi-kinase inhibitor rigosertib (ON 01910.Na) induces mitotic arrest and apoptosis in myeloblasts, while sparing normal cells. The purpose of this study was to determine the pharmacokinetic profile, maximum-tolerated dose (MTD), safety, and clinical activity of an oral formulation of rigosertib in patients with myelodysplastic syndromes (MDS). For pharmacokinetic studies, patients received rigosertib in single escalating weekly doses. To determine the MTD, patient cohorts received escalating doses of rigosertib twice daily for 14 d of a 21-d cycle. Overall, 37 patients were treated. Rigosertib exposure increased with escalating oral doses. Mean absolute oral bioavailability ranged from 13·9% (fed) to 34·8% (fasting) in 12 patients treated at the 560 mg b.i.d. dose level. Dose-limiting toxicity (grade 3 dysuria and shortness of breath) occurred at the 700 mg b.i.d. dose. Five patients experienced grade 3 non-haematological toxicity, including symptoms of urothelial inflammation, hypotension and syncope, fatigue and abdominal pain. Encouraging signs of clinical activity included two bone marrow complete remissions in refractory anaemia with excess blasts type 1 patients previously treated with azacitidine. In addition, four patients each achieved transfusion independence and haematological improvements. In conclusion, oral rigosertib is bioavailable and well tolerated, and has clinical activity in patients with MDS. PMID:23789936

  2. Response to Androgen Therapy in Patients with Dyskeratosis Congenita

    PubMed Central

    Khincha, Payal P.; Wentzensen, Ingrid M.; Giri, Neelam; Alter, Blanche P.; Savage, Sharon A.

    2014-01-01

    Dyskeratosis congenita (DC) is an inherited bone marrow failure syndrome and telomere biology disorder characterized by dysplastic nails, reticular skin pigmentation and oral leucoplakia. Androgens are a standard therapeutic option for bone marrow failure in those patients with DC who are unable to undergo haematopoietic stem cell transplantation, but there are no systematic data on its use in those patients. We evaluated haematological response and side effects of androgen therapy in 16 patients with DC in our observational cohort study. Untreated DC patients served as controls. Seventy percent of treated DC patients had a haematological response with red blood cell and/or platelet transfusion independence. The expected age-related decline in telomere length was noted in androgen-treated patients. All treated DC patients had at least one significant lipid abnormality. Additional treatment-related findings included a significant decrease in thyroid binding globulin, accelerated growth in pre-pubertal children and splenic peliosis in two patients. Liver enzymes were elevated in both androgen-treated and untreated patients, suggesting underlying liver involvement in DC. This study suggests that androgen therapy can be effectively used to treat bone marrow failure in DC, but that side effects need to be closely monitored. PMID:24666134

  3. Ferric chondroitin 6-sulfate (Condrofer): a new potent antianaemic agent with a favourable pharmacokinetic profile.

    PubMed

    Barone, D; Orlando, L; Vigna, E; Baroni, S; Borghi, A M

    1988-01-01

    One and 3 mg/kg iron as Condrofer**, a new soluble formulation of this metal, and 1 mg/kg iron as Proteoferrina*** or ferritin were given orally for 4 weeks to male rats in which severe experimental anaemia had previously been induced (by iron-deficient diet and repeated bleedings). Haematological (erythrocyte count, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, reticulocytes and leukocytes) and blood chemistry (sodium, potassium, iron and total protein) parameters were checked weekly and at the end of the drug administration period. Clinical and behavioral signs, body weight, food intake and necroscopic observations were also recorded. Condrofer time- and dose-dependently improved the general blood picture, the clinical data and the autoptic findings to the point of making these animals significantly approach control rats, save for one parameter, sideremia, which after 4 weeks of treatment remained lower than normal. The most plausible explanation would seem that the severe anaemia interfered both with the physiological iron storage and with the iron-dependent mitochondrial enzymatic systems. Iron (1 mg/kg) daily as Proteoferrina or ferritin was significantly less effective than when this metal was administered as Condrofer, since all the haematological parameters and the clinical, behavioral signs and necroscopic observations were less favourable. The more complete reversal of anaemia in the rats that received Condrofer is, most probably, due to the higher bioavailability of iron administered under this formulation, as demonstrated by iron kinetics after equidoses of iron as Condrofer and Proteoferrina. PMID:3148451

  4. Molecular Prevalence of Babesia bigemina and Trypanosoma evansi in Dairy Animals from Punjab, India, by Duplex PCR: A Step Forward to the Detection and Management of Concurrent Latent Infections

    PubMed Central

    Sharma, Amrita; Singla, Lachhman Das; Tuli, Ashuma; Kaur, Paramjit; Batth, Balwinder Kaur; Javed, Mohammed; Juyal, Prayag Dutt

    2013-01-01

    Specific duplex polymerase chain reaction (PCR) was employed on 411 (386 cattle and 25 buffaloes) blood samples of dairy animals from 9 districts of Punjab, India, for simultaneous detection of Babesia bigemina and Trypanosoma evansi. The results were compared and correlated with conventional Giemsa stained thin blood smear (GSTBS) examination and haematological alterations to know the clinical status and pathogenicity of infections. The Bg3/Bg4 and TR3/TR4 primers were used in duplex PCR for B. bigemina and T. evansi amplified products of 689?bp and 257?bp, respectively. The overall prevalence by duplex PCR was found to be 36.49, 2.43, and 3.41% for T. evansi, B. bigemina, and dual infection, respectively. A more significant difference was observed for dual infection status (P ? 0.005) as compared to T. evansi (P ? 0.05) and B. bigemina (P ? 0.01) among various districts under study. A very low prevalence of T. evansi (0.73%) and B. bigemina (0.48%) was seen by GSTBS. The highly sensitive, specific, and cost-effective duplex PCR was able to detect latent T. evansi and B. bigemina infection in cattle and buffaloes. Haematological evaluation revealed marked pathology in B. bigemina infected group and in dual infected group in contrast to that infected with T. evansi alone. PMID:24069605

  5. Sub-clinical diseases affecting performance in Standardbred trotters: diagnostic methods and predictive parameters.

    PubMed

    Richard, Eric A; Fortier, Guillaume D; Pitel, Pierre-Hugues; Dupuis, Marie-Capucine; Valette, Jean-Paul; Art, Tatiana; Denoix, Jean-Marie; Lekeux, Pierre M; Erck, Emmanuelle Van

    2010-06-01

    The objectives of this study were to determine the prevalence of sub-clinical diseases in poorly-performing Standardbred horses, compare their physiological response to exercise with control horses, and identify predictive parameters of poor-performance. Fifty horses underwent thorough clinical and ancillary examinations, including haematological and biochemical evaluation, Doppler echocardiography, standardised exercise tests (SETs) on both treadmill and racetrack, treadmill video-endoscopy and collection of respiratory fluids. Most of the poorly-performing horses exhibited many concomitant diseases. The most frequently diagnosed problems involved the lower and upper respiratory tract and the musculoskeletal system. Poor-performers had lower speeds at a blood lactate (LA) concentration of 4mmol/L (V(LA4)) and a heart rate (HR) of 200bpm (V(200)) on treadmill and racetrack, as well as lower values for haematological parameters, plasma angiotensin-converting enzyme and antioxidants, compared to control horses. Problems of the respiratory system were the most frequently diagnosed sub-clinical diseases affecting performance. SETs, together with some blood markers, may be useful as a non-specific diagnostic tool for early detection of diseases that may affect performance. PMID:19477143

  6. Investigation of diseases through red blood cells' shape using photoacoustic response technique

    NASA Astrophysics Data System (ADS)

    Biswas, Deblina; Gorey, Abhijeet; Chen, Goerge C. K.; Sharma, Norman; Vasudevan, Srivathsan

    2015-03-01

    Photoacoustic (PA) imaging is a non-invasive real-time technique, widely applied to many biomedical imaging studies in the recent years. While most of these studies have been focussed on obtaining an image after reconstruction, various features of time domain signal (e.g. amplitude, width, rise and relaxation time) would provide very high sensitivity in detecting morphological changes in cells during a biological study. Different haematological disorders (e.g., sickle cell anaemia, thalassemia) exhibit significant morphological cellular changes. In this context, this study explores the possibility of utilizing the developed photoacoustic response technique to apply onto blood samples. Results of our preliminary study demonstrate that there is a significant change in signal amplitude due to change in concentration of the blood. Thus it shows the sensitivity of the developed photoacoustic technique towards red blood cell count (related to haematological disease like anaemia). Subsequently, morphological changes in RBC (i.e. swollen and shrunk compared to normal RBC) induced by hypotonic and hypertonic solutions respectively were also experimented. The result shows a distinct change in PA signal amplitude. This would serve as a diagnostic signature for many future studies on cellular morphological disorders.

  7. Raised levels of interleukin 6 in systemic lupus erythematosus correlate with anaemia

    PubMed Central

    Ripley, B; Goncalves, B; Isenberg, D; Latchman, D; Rahman, A

    2005-01-01

    Objectives: Raised levels of the cytokines interleukin (IL) 6 and IL10 have been reported in patients with systemic lupus erythematosus (SLE). Objective: To determine if levels of IL6 and IL10 correlate with organ/system-specific disease activity in SLE, using the British Isles Lupus Assessment Group (BILAG) Disease Activity Index. Methods: Levels of IL6 and IL10 in serum samples from 171 patients with SLE and 50 normal controls were determined by enzyme linked immunosorbent assay (ELISA). Levels of cytokines in individual patients with SLE were compared with the presence or absence of active disease in eight organ/systems using the BILAG index. Results: Levels of IL6 were significantly higher (p = 0.005) in patients with active compared with inactive haematological disease, as scored by the BILAG index. Further analysis showed that this association was dependent on an inverse correlation (p = 0.002, r = –0.26) between IL6 levels and haemoglobin levels in patients with SLE. In contrast, IL10 levels did not correlate with individual organ/system disease activity. Conclusions: Raised levels of IL6 in SLE may influence the development of anaemia in this disease. These findings are in agreement with an increasing number of studies, which support physiological links between IL6 and anaemia. Importantly, with the exception of the haematological system, our studies do not provide evidence of any individual organ/system which would respond to therapeutic manipulation of either IL6 or IL10 levels. PMID:15897306

  8. Hypolipidaemic effect of crude extract from Carpobrotus rossii (pigface) in healthy rats.

    PubMed

    Pirie, Adam D; Davies, Noel W; Ahuja, Kiran D K; Adams, Murray J; Shing, Cecilia M; Narkowicz, Christian; Jacobson, Glenn A; Geraghty, Dominic P

    2014-04-01

    Carpobrotus rossii (CR) was used by the Aboriginal population and early European settlers both as a food and therapeutic agent. Based on the presence of flavonoids in CR and results from our previous in vitro investigations, this study aimed to determine whether consumption of CR crude leaf extract: (a) affected lipoprotein profile, resting glucose, systolic blood pressure and vascular function, and (b) produced toxic effects (haematological measures, organ weight) in healthy rats. Male Hooded-Wistar rats (~230 g) were supplemented for 4 weeks with CR extract in their drinking water (35 mg/kg body weight daily). CR extract produced a significant decrease (18%, p=0.033) in atherogenic lipoproteins (but not high density lipoprotein). CR supplemented animals showed no signs of haematological toxicity and body and organ weight, daily fluid and food consumption and in vitro vascular responsiveness were similar for both groups. CR also increased (40%, p=0.049) the renal concentration of 3-hydroxy-3-methylglutaric acid (HMG), consistent with HMG-containing CR flavonoids being bioavailable, and therefore possessing the potential to interfere with cholesterol synthesis pathways. CR extract appears to be safe to ingest and may reduce cardiovascular risk. PMID:24480043

  9. Eltrombopag: a review of its use in patients with severe aplastic anaemia.

    PubMed

    McCormack, Paul L

    2015-04-01

    Eltrombopag (Promacta(®)) is an orally active thrombopoietin receptor agonist recently approved in the US for the treatment of patients with severe aplastic anaemia who have had an insufficient response to immunosuppressive therapy. This article reviews the efficacy and tolerability of eltrombopag in this indication and overviews its pharmacological properties. Eltrombopag does not compete with thrombopoietin and binds to a different site on the receptor, producing additive effects. It stimulates haematopoietic stem cells and promotes haematopoietic recovery in patients with aplastic bone marrow. Eltrombopag increased platelet counts and can also increase red blood cell and neutrophil counts. In patients with severe aplastic anaemia refractory to prior immunosuppressive therapy, oral eltrombopag at dosages ?150 mg once daily for 12-16 weeks produced a haematological response in at least one cell lineage in 40 % of patients. Trilineage responses were achieved in nearly one-half of the responders during extended treatment. In robust responders, stable haematological counts were maintained after eltrombopag discontinuation. Eltrombopag was generally well tolerated, with increased liver transaminases as the only dose-limiting toxicity. Clonal cytogenetic abnormalities were observed in 19 % of patients and dysplasia in 5 % of patients. PMID:25700916

  10. Rapidly progressive cerebellar ataxia in West Wales.

    PubMed

    Ali, Khalid; Amin, Reem; Yoganathan, Kathir G; Powell, Rob

    2013-01-01

    Progressive multifocal leucoencephalopathy (PML) is a severe demyelinating disease of the central nervous system that is caused by the JC virus infection. It is often fatal or severely disabling. PML exclusively happens in the context of cell-mediated immunosuppression. Prior to the era of HIV, PML was mainly confined to patients with haematological malignancies and rheumatological diseases. The HIV epidemic in the early eighties led to massive expansion in the incidence and prevalence of the disease. PML has also been recognised to happen due to treatment with monoclonal antibodies such as natalizumab, which is used as a disease-modifying agent for relapsing remitting multiple sclerosis and other monoclonal antibodies used in dermatological and haematological conditions. The clinical picture is that of cognitive decline, visual disturbance and hemiparesis. The correct clinicoradiological picture combined with demonstrating the JC virus DNA in the cerebrospinal fluid (CSF) using PCR (PMR) is enough to establish the diagnosis. Brain biopsy is rarely needed. Immune reconstitution represents the mainstay in the treatment of PML. We present a case of a 47-year-old man who presented with progressive cerebellar ataxia. Investigations confirmed PML. He was found to be HIV positive. We also review the literature. PMID:24265345

  11. Immune checkpoint inhibition in lymphoid disease.

    PubMed

    Eyre, Toby A; Collins, Graham P

    2015-08-01

    It has long been understood that the immune system has intrinsic anti-tumour activity in humans, and that a key mechanism of tumour progression is the ability of a tumour to escape this immune surveillance. A number of attempts have been made to harness this anti-tumour immunity in both solid tumour oncology and haematological malignancies with variable success. Examples include the use of allogeneic stem cell transplantation and donor lymphocyte infusion in haematological cancer and vaccine studies in solid tumours. Enhanced signalling of the Programmed cell death-1 (PDCD1, PD-1)/cytotoxic T-lymphocyte-associated protein 4 (CTLA4) 'immune checkpoint' pathway has emerged recently as a critical mechanism by which tumours can escape the natural anti-tumour immune response. As such, novel therapies have been developed to help enhance this natural immunity by switching off the PDCD1/CTLA4 immune checkpoint pathway. The following review will discuss the pathobiology of these pathways and the exciting new data now available in lymphoid malignancies. PMID:25824455

  12. Epidemiology and outcomes of patients with invasive mould infections: a retrospective observational study from a single centre (2005-2009).

    PubMed

    Klingspor, Lena; Saaedi, Baharak; Ljungman, Per; Szakos, Attila

    2015-08-01

    Invasive mould infection (IMI) is an important cause of morbidity and mortality in immunocompromised patients. However, Swedish epidemiology data are lacking. The aim of this study was to investigate the epidemiology and outcome of IMI. Cases of proven/probable IMI at Karolinska University Hospital, Stockholm, from 2005 to 2009, were included. A total of 100 patients with 104 episodes of IMI were enrolled. Identified isolates included 101 mould isolates. The majority of the isolates were Aspergillus spp. (74.3%), followed by Mucorales spp. (13.9%), Fusarium spp. (4.9%) and other mould spp. (6.9%). In 13% of the episodes, more than one mould caused the IMI. The lung was most often affected (88.5%). The most frequent underlying disease was haematological malignancies (70%). Following diagnosis, 83.7% initially received antifungal monotherapy, 9.6% received combination therapy and 6.7% no treatment. The overall 90-day and 1-year overall survival was 49% and 46% respectively. Survival at 90 days post diagnosis was 71.4% in the solid tumour cohort, 62.5% in patients with solid organ transplants, 43.5% in haematological malignancy (HMs) and 37% in those undergoing allogeneic haematopoietic stem cell transplantation (HSCT). Overall survival was poor in the studied cohort, but is variable among different host categories, with particular opportunities for improvement in patients with underlying HMs and allogeneic HSCT. PMID:26152371

  13. Long-term follow-up and second malignancies in 487 patients with hairy cell leukaemia.

    PubMed

    Cornet, Edouard; Tomowiak, Cécile; Tanguy-Schmidt, Aline; Lepretre, Stéphane; Dupuis, Jehan; Feugier, Pierre; Devidas, Alain; Mariette, Clara; Leblond, Véronique; Thiéblemont, Catherine; Validire-Charpy, Patricia; Sutton, Laurent; Gyan, Emmanuel; Eisenmann, Jean-Claude; Cony-Makhoul, Pascale; Ysebaert, Loďc; Troussard, Xavier

    2014-08-01

    A large, multicentre, retrospective survey of patients with hairy cell leukaemia (HCL) was conducted in France to determine the frequency of second malignancies and to analyse the long-term effects of the established purine nucleoside analogues (PNAs), cladribine and pentostatin. The survey retrospectively reviewed the medical history of patients and their immediate family, clinical and biological presentation at the time of HCL diagnosis, treatment choice, response to treatment, time to relapse and cause of death. Data were collected for 487 patients with HCL. Of the patients included in the survey, 18% (88/487) had a familial history of cancers, 8% (41/487) presented with malignancies before HCL diagnosis and 10% (48/487) developed second malignancies after HCL was diagnosed. An excess incidence of second malignancies was observed, with a standardized incidence ratio (SIR) of 1·86 (95% confidence interval (CI): 1·34-2·51), with no significant difference between PNAs. For second haematological malignancies alone, the SIR was markedly increased at 5·32 (95% CI: 2·90-8·92). This study highlights the high frequency of cancers in HCL patients and their family members. The frequency of second malignancies is notably increased, particularly for haematological malignancies. The respective role of pentostatin and cladribine in the development of second malignancies is debatable. PMID:24749839

  14. Preclinical antitumor activity of ST7612AA1: a new oral thiol-based histone deacetylase (HDAC) inhibitor

    PubMed Central

    Milazzo, Ferdinando Maria; De Santis, Rita; Gaudio, Eugenio; Kwee, Ivo; Rinaldi, Andrea; Pace, Silvia; Carollo, Valeria; Giannini, Giuseppe; Bertoni, Francesco

    2015-01-01

    ST7612AA1 (property of Sigma-Tau), a thioacetate-? (?-lactam amide) derivative, is a potent, second generation, oral pan-histone deacetylase inhibitor (HDACi). Aim of the study was to assess the efficacy of ST7612AA1 in solid and haematological tumors, and to characterize its mechanism of action. In vitro, ST7612AA1 potently inhibited different class I and class II HDACs, leading to restore the balance of both histone and non-histone protein acetylation. In vivo, it induced significant anti-tumor effects in xenograft models of lung, colon, breast and ovarian carcinomas, leukemia and lymphoma. This was likely due to the modulation of different HDAC substrates and induction of transcriptional changes with respect to several genes involved in key processes, such as cell cycle regulation, DNA damage checkpoints, immune response, cell adhesion and epithelial-to-mesenchymal transition. PK analysis confirmed the pro-drug nature of ST7612AA1, which is rapidly absorbed and converted to ST7464AA1 after a single oral dose in mice. ST7612AA1 was selected from a novel generation of oral HDAC inhibitors. Its high efficacy correlated with its potent and selective inhibitory activity of HDAC and was combined with a favorable pharmacodynamics profile. These aspects support a clinical development of ST7612AA1 towards a broad spectrum of human solid and haematologic malignancies. PMID:25671299

  15. Inhibition of Ehrlich ascites carcinoma by Manilkara zapota L. stem bark in Swiss albino mice

    PubMed Central

    Osman, M Abu; Rashid, M Mamunur; Aziz, M Abdul; Habib, M Rowshahul; karim, M Rezaul

    2011-01-01

    Objective To evaluate the antitumor activity of Manilkara zapota (M. zapota) L. stem bark against Ehrlich ascites carcinoma (EAC) in Swiss albino mice. Methods The in vivo antitumour activity of the ethyl acetate extract of stem bark of M. zapota L. (EASM) was evaluated at 50, 100 and 200 mg/kg bw against EAC using mean survival time. After administration of the extract of M. zapota, viable EAC cell count and body weight in the EAC tumour hosts were observed. The animal was also observed for improvement in the haematological parameters (e.g., heamoglobin content, red and white blood cells count and differential cell count) after EASM treatment. Results Intraperitoneal administration of EASM reduced viable EAC cells, increased the survival time, and restored altered haematological parameters. Significant efficacy was observed for EASM at 100 mg/kg dose (P<0.05). Conclusions It can be concluded that the ethyl acetate extract of stem bark of M. zapota L. possesses significant antitumour activity. PMID:23569811

  16. Response to lenalidomide in myelodysplastic syndromes with del(5q): influence of cytogenetics and mutations.

    PubMed

    Mallo, Mar; Del Rey, Mónica; Ibáńez, Mariam; Calasanz, M José; Arenillas, Leonor; Larráyoz, M José; Pedro, Carmen; Jerez, Andrés; Maciejewski, Jaroslaw; Costa, Dolors; Nomdedeu, Meritxell; Diez-Campelo, María; Lumbreras, Eva; González-Martínez, Teresa; Marugán, Isabel; Such, Esperanza; Cervera, José; Cigudosa, Juan C; Alvarez, Sara; Florensa, Lourdes; Hernández, Jesús M; Solé, Francesc

    2013-07-01

    Lenalidomide is an effective drug in low-risk myelodysplastic syndromes (MDS) with isolated del(5q), although not all patients respond. Studies have suggested a role for TP53 mutations and karyotype complexity in disease progression and outcome. In order to assess the impact of complex karyotypes on treatment response and disease progression in 52 lenalidomide-treated patients with del(5q) MDS, conventional G-banding cytogenetics (CC), single nucleotide polymorphism array (SNP-A), and genomic sequencing methods were used. SNP-A analysis (with control sample, lymphocytes CD3+, in 30 cases) revealed 5q losses in all cases. Other recurrent abnormalities were infrequent and were not associated with lenalidomide responsiveness. Low karyotype complexity (by CC) and a high baseline platelet count (>280 × 10(9) /l) were associated with the achievement of haematological response (P = 0·020, P = 0·013 respectively). Unmutated TP53 status showed a tendency for haematological response (P = 0·061). Complete cytogenetic response was not observed in any of the mutated TP53 cases. By multivariate analysis, the most important predictor for lenalidomide treatment failure was a platelet count <280 × 10(9) /l (Odds Ratio = 6·17, P = 0·040). This study reveals the importance of a low baseline platelet count, karyotypic complexity and TP53 mutational status for response to lenalidomide treatment. It supports the molecular study of TP53 in MDS patients treated with lenalidomide. PMID:23614682

  17. 2-Arylbenzimidazoles as antiviral and antiproliferative agents. Part 1.

    PubMed

    Vitale, G; Carta, A; Loriga, M; Paglietti, G; La Colla, P; Busonera, B; Collu, D; Loddo, R

    2008-11-01

    Being involved in an anti-Flaviviridae Project, and because of the role played by benzimidazole derivatives as promising inhibitors of the HCV helicase and RNA polymerase, as well as of the Zn finger transcription factor, we synthesized a new series of 2-arylbenzimidazoles and evaluated them for antiviral activity, as well as for antiproliferative activity. Compounds were tested in cell-based assays against viruses representative of: i) two of the three genera of the Flaviviridae family, i.e. Flaviviruses and Pestiviruses; ii) other RNA virus families, such as Retroviridae, Picornaviridae, Paramyxoviridae, Rhabdoviridae and Reoviridae; iii) two DNA virus families (Herpesviridae and Poxviridae). Compounds 15, 28 and 29 resulted moderately active only against Yellow Fever Virus (a Flavivirus) (range 6-27 microM), whereas none of the title benzimidazoles showed any antiviral activity at concentrations not cytotoxic for the resting cell monolayers. Compounds were also tested for antiproliferative activity against a panel of exponentially growing cell lines derived from human haematological and solid tumors. Several new benzimidazoles turned out active. Among them, compound 27 was the most potent against human haematologic and solid tumor cells and turned out to be as potent as Etoposide and more potent than 6-mercaptopurine (6-MP), used as reference antitumor agents. PMID:18991746

  18. Toxic effects of lead exposure in Wistar rats: involvement of oxidative stress and the beneficial role of edible jute (Corchorus olitorius) leaves.

    PubMed

    Dewanjee, Saikat; Sahu, Ranabir; Karmakar, Sarmila; Gangopadhyay, Moumita

    2013-05-01

    Lead (Pb) is considered to be a multi-target toxicant. The present study was undertaken to evaluate the protective effect of aqueous extract of Corchorus olitorius leaves against Pb-acetate induced toxic manifestation in blood, liver, kidney, brain and heart of Wistar rats. The Pb-acetate (5mg/kg body weight) treated rats exhibited a significant inhibition of co-enzymes Q, antioxidant enzymes and reduced glutathione levels in the tissues. In addition, the extent of lipid peroxidation, DNA fragmentation and haematological parameters were significantly altered in the Pb-acetate treated rats as compared to control. Simultaneous administration of test extract (25, 50 and 100mg/kg body weight), could significantly restore the biochemical and haematological parameters near to the normal status through antioxidant activity and/or by preventing bioaccumulation of Pb within the tissues of experimental rats. Presence of substantial quantity of phenolics and flavonoids in the extract may be responsible for the observed protective role against Pb-intoxication. PMID:23291325

  19. Cadmium induced pathophysiology: prophylactic role of edible jute (Corchorus olitorius) leaves with special emphasis on oxidative stress and mitochondrial involvement.

    PubMed

    Dewanjee, Saikat; Gangopadhyay, Moumita; Sahu, Ranabir; Karmakar, Sarmila

    2013-10-01

    The present study was undertaken to evaluate the protective effect of aqueous extract of Corchorus olitorius leaves (AECO) against CdCl? intoxication. In vitro bioassay on isolated mice hepatocytes confirmed dose dependent cytoprotective effect of AECO. The CdCl? (30 ?M) exhibited a significantly increased levels of lipid peroxidation, protein carbonylation along with the reduction of antioxidant enzymes and reduced glutathione levels in hepatocytes. AECO (200 and 400 ?g/ml) + CdCl? (30 ?M) could significantly restore the aforementioned oxidation parameters in hepatocytes. Beside this, AECO could significantly reduce Cd-induced increase in Bad/Bcl-2 ratio and the over-expression of NF-?B, caspase 3 and caspase 9. In in vivo assay, CdCl? (4 mg/kg body weight, for 6 days) treated rats exhibited a significantly increased intracellular Cd accumulation, oxidative stress and DNA fragmentation in the organs. In addition, the haematological parameters were significantly altered in the CdCl? treated rats. Simultaneous administration of AECO (50 and 100 mg/kg body weight), could significantly restore the biochemical, antioxidant and haematological parameters near to the normal status. Histological studies of the organs supported the protective role of jute leaves. Presence of substantial quantity of phenolic compounds and flavonoids in extract may be responsible for overall protective effect. PMID:23891759

  20. Burkitt's lymphoma presenting with hypopituitarism: a case report and review of literature

    PubMed Central

    Sobah, Shahada A H

    2014-01-01

    Summary Hypopituitarism is a rare presentation of Burkitt's lymphoma (BL). The purpose of this report is to present a case of BL presenting with panhypopituitarism and to review other case reports of lymphoma presenting with pituitary dysfunction to highlight the distinguishing features of these cases from other benign aetiologies of pituitary dysfunction such as non-functioning pituitary adenomas. We reviewed a total of 11 cases of lymphoma presenting with pituitary dysfunction published from 1998 to 2013 including the present case. The demographics, clinical presentations, laboratory features, radiological findings, histological diagnosis, treatment administered and outcomes were described. Of the total number of patients, 45.5% of the cases had diffuse large B-cell lymphoma while 27.3% had BL. Anterior pituitary dysfunction was more common than posterior pituitary dysfunction at presentation. The other common associated presenting symptoms were painful ophthalmoplegia, cranial nerve palsies and constitutional symptoms. Hypothalamic–pituitary abnormalities were often demonstrated radiologically to be associated with cavernous sinus and/or stalk involvement. All patients who completed immunochemotherapy responded haematologically. Pituitary dysfunction also improved in most cases although the recovery tended to be partial. In conclusion, a high index of suspicion of underlying malignancy, such as lymphoma, should be present in patients presenting with acute pituitary dysfunction associated with painful ophthalmoplegia, rapidly evolving neurological features, radiological features atypical of a pituitary adenoma and constitutional symptoms. An early diagnosis is essential as prompt initiation of definitive therapy will induce disease remission and recovery of pituitary dysfunction. Learning points Hypopituitarism may be the presenting symptom of lymphoma in the absence of associated overt symptoms or signs of a haematological malignancy resulting in delay in diagnosis and institution of treatment.Pituitary dysfunction due to tumour infiltration has a greater tendency to involve the posterior pituitary and infundibulum resulting in diabetes insipidus and hyperprolactinaemia compared with a non-functioning pituitary adenoma.The common associated symptoms of hypopituitarism due to lymphoma infiltration of the hypothalamic–pituitary system include painful ophthalmoplegia, cranial nerve palsies and constitutional symptoms.Radiological abnormalities of the hypothalamic–pituitary region are usually present and often associated with cavernous sinus or stalk involvement.With early institution of definitive treatment, both haematological response and improvement of pituitary dysfunction are expected although the reversal of hypopituitarism tends to be partial and delayed.A high index of suspicion of underlying malignancy such as lymphoma should be present in patients presenting with acute pituitary dysfunction associated with painful ophthalmoplegia, radiological features atypical of pituitary adenomas and constitutional symptoms to enable early diagnosis and prompt initiation of definitive therapy. PMID:25045522

  1. A multidisciplinary approach for improving services in primary care: randomised controlled trial of screening for haemoglobin disorders

    PubMed Central

    Modell, Michael; Wonke, Beatrix; Anionwu, Elizabeth; Khan, Maren; Tai, Sharon See; Lloyd, Michael; Modell, Bernadette

    1998-01-01

    Objective: To investigate the feasibility of improving screening for carriers of haemoglobin disorders in general practice by using a nurse facilitator to work with primary care teams and the relevant haematology laboratories; to identify problems in communication between all those involved in delivering the service, and to implement solutions. Design: Two year, practice based randomised controlled trial. Setting: North London area where 29% of residents and 43% of births are in ethnic groups at risk for haemoglobin disorders. Subjects: 26 of the 93 practices using the services of the area’s haematology laboratory agreed to take part and were randomly divided into control and intervention practices. Main outcome measure: Change in number of requests for screening tests for haemoglobin disorders made by control and intervention practices in baseline and intervention years. Results: The number of screening tests requested varied from 0-150 in the 93 practices in the baseline year. Study practices tended to have made a moderate number of requests (10-50) during this period. During the intervention year intervention practices made 292 more requests (99% increase) and control practices made 74 fewer requests (23% decrease; P=0.001 for difference in median change). Four practices, three of which were singlehanded, accounted for 75% of the increase. The number of requests from intervention practices, adjusted for baseline requests, was 3.2 times higher than control practices (P<0.0001). Conclusion: General practitioners and practice nurses are willing to undertake a new genetic screening service (or expand an existing one) if they are persuaded that it benefits the health of a significant proportion of their practice population. They need appropriate tools (for example, information materials for carriers and groups at risk), and the laboratory must be sensitive to their needs. Preconceptional carrier screening and counselling need to be coupled with antenatal screening. Key messagesMany couples at risk of conceiving a child with a haemoglobin disorder are identified too late in pregnancy for prenatal diagnosis to be acceptable to themThese couples need to be identified before conception, or as soon as they report a pregnancy to their general practitionerScreening for haemoglobin disorders in primary care can be enhancedA multidisciplinary approach and a haematology laboratory responsive to the needs of local practitioners are importantThe nurse facilitator was particularly successful in persuading singlehanded practitioners practising in areas with a high prevalence of ethnic minorities to increase their testing for carriers PMID:9740569

  2. A subchronic toxicity study of Rokanol B-2 in rats.

    PubMed

    Krysiak, B; Rydzy?ski, K; Stetkiewicz, J; Stetkiewicz, I

    1996-01-01

    The toxicity of Rokanol B-2 was assessed following its administration to rats via oral gavage. A preliminary study of acute toxicity was performed to determine suitable dosing regimen/dose levels for future repeated-dose toxicity studies. For this purpose rats (15 males and 7 females) received single doses of 2,000, 1,500, 1,000 or 500 mg/kg and were killed after 2 or 14 days. No deaths occurred during the observation time. Dose-related irritation to the stomach mucosa was found. For the subchronic toxicity assessment, Rokanol B-2 was administered at daily doses of 8, 40 or 200 mg/kg to 59 male and 56 female Wistar rats by oral gavage, 5 days per week for 90 days. An interim experiment was performed after 28 days of dosing. Five animals/sex/group were terminated and necropsied. Blood samples for clinical chemistry and haematology were obtained and lungs, heart, adrenals, kidneys, liver, spleen, stomach, intestine, testes (males), uterus and ovaries (females) were weighed and prepared for histopathological examination. After 90 days of dosing all remaining animals were killed and necropsied. Blood samples were taken for evaluation of haematology and clinical chemistry, and selected organs (same as above) prepared for subsequent histological examination. In the high-dose group (200 mg/kg/day) a statistically significant reduction in body weight gain and food consumption, an increased weight of the liver in the males and disturbances in haematological parameters in the females were observed. Ulcerations of the mucous membrane of the stomach and hyperkeratosis, and in a few cases, pseudopapillomatous epithelial proliferation of foregaster and exudate in the submucosa of the stomach were noted. In the mid-dose group (40 mg/kg/day) some disturbances in hematological parameters in females and histopathological changes in rats of both sexes similar, but to a lesser degree, to changes observed in high-dose animals were indicated. In the low-dose group (8 mg/kg/day) no significant treatment-related effects were observed. The results of this study indicate that Rokanol B-2 possessed an overall low degree of systemic toxicity when administered orally to rats for 90 days. The NOAEL, observed in this study, was estimated as 8 mg/kg/day. PMID:9117194

  3. A Study of Alternate Biomarkers in HIV Disease and Evaluating their Efficacy in Predicting T CD4+ Cell Counts and Disease Progression in Resource Poor Settings in Highly Active Antiretroviral Therapy (HAART) Era

    PubMed Central

    Ramana, K V; Sabitha, V; Rao, Ratna

    2013-01-01

    Introduction: Human Immunodeficiency Virus (HIV), the causative agent of AIDS, has been a challenge to medical fraternity since it was first discovered in 1983. About 40 million people are living with HIV infection globally and 99% of the infected people are in south East Asia (SEA). Traditionally, HIV disease and progression, initiation of HAART and response to therapy is monitored by assessing in regular intervals, the T CD4+ cell counts and plasma HIV/RNA viral load. Resource poor, low and low – middle income group countries still have no finances to acquire infrastructure and scientific technology for performing such tests. Objectives: Since very few studies are available, they have demonstrated the role of alternate biomarkers that can be used to predict CD4 cell counts and thereby, monitor HIV disease progression and HAART. We aimed to measure certain haematological parameters in HIV seropositive patients and to evaluate their efficacy in predicting TCD4+ cell counts. Methods: The study group included 250 HIV seropositive patients with an age range of 18-65 years. 140(56%) males and 110(44%) females were included in the study. Absolute TCD4+cell counts and CD8+T cell counts were measured by using a flow cytometer. (MMWR Recommendations and Reports, 1992) TLC; HB%, AEC and ESR were estimated by using conventional haematological methods. CRP was evaluated by latex agglutination test (Immuno CRP Latex Agglutination Test). Results: Among the tested haematological markers, a TLC of <1800 cells/mm3 showed high specificity (100%) in predicting CD4 counts of < 200 cells/mm3, with an accuracy of 61.46%. Haemoglobin and Absolute Eosinophilic counts showed high specificities of 84.09% and 94.32% respectively in predicting CD4 counts which were below 350 cells/mm3. ESR with 98.98% sensitivity and AEC which had 83.67% sensitivity were able to predict CD4 counts of <200 cells/mm3. Conclusion: Among the tested biomarkers, it was seen that Absolute Eosinophilic counts of more than 550 cells/mm3, Blood Haemoglobin which was less than 10 g%, ESR which measured more than 20 mm, CRP values of >1.2 and TLC of <1800 cells/mm3 could be helpful in predicting CD4 cell counts of < 350 and <200 cells/mm3. PMID:23998059

  4. Ingestion of colostrum from specific cows induces Bovine Neonatal Pancytopenia (BNP) in some calves

    PubMed Central

    2011-01-01

    Background Since 2006, cases of haemorrhagic diathesis in young calves have been observed with a much higher incidence than previously known. The syndrome, now uniformly called Bovine Neonatal Pancytopenia (BNP), is characterized by multiple (external and internal) haemorrhages, thrombocytopenia, leukocytopenia, and bone marrow depletion. Although various infectious and toxicological causes of bleeding disorders in calves have been ruled out, the aetiology of BNP remains unknown. However, field observations have led to the hypothesis that the aetiological principle may be transmitted to calves via colostrum. The objective of the present study was to verify whether ingestion of colostrum from dams of known BNP calves can elicit signs of BNP and typical haematological findings in conveniently selected neonatal calves. Six such calves received one feeding of colostrum (or a mixture of colostrum batches) from dams of known BNP calves. As controls, another six conveniently selected calves from herds which had never had a BNP case received one feeding of colostrum from their own dams. Haematological and clinical parameters were monitored. Results One of the six experimental calves never showed any haematological, clinical or pathological evidence of BNP. In the other five calves, thrombocyte and leukocyte counts dropped within a few hours following ingestion of colostrum. Of those, three calves developed clinical signs of BNP, their post-mortem examination revealed bone marrow depletion. Of the remaining two calves, a pair of mixed twins, marked thrombocytopenia and recurrent leukocytopenia was evident in one, in which only slight changes in the bone marrow were detected, while in the other thrombocyte counts dropped, but rebounded later, and no bone marrow changes were noted. Thrombocyte counts of the experimental calves were statistically significantly lower than those of the control calves at 2 hours post ingestion of colostrum and at every sampling point between 9 hours and 8 days postcolostral. Leucocyte counts of the experimental calves were statistically significantly lower than those of control calves at 2 hours post ingestion of colostrum and 3-7 days postcolostral. Conclusions BNP can be induced in some calves by ingestion of colostrum from cows that have given birth to BNP calves. PMID:21333009

  5. Haematopoietic cancer mortality among vehicle mechanics.

    PubMed Central

    Hunting, K L; Longbottom, H; Kalavar, S S; Stern, F; Schwartz, E; Welch, L S

    1995-01-01

    OBJECTIVE AND METHODS--This historical cohort study investigated causes of death among car and mobile equipment mechanics in the District of Columbia's Department of Public Works. Men who were employed for at least one year between 1977 and 1989 were eligible for inclusion in the cohort; follow up was up to the end of 1991. Three cases of leukaemia (index cases) had been reported among these workers before the inception of this study. This research was undertaken to estimate the relative risk of haematological cancer among mechanics working for the District of Columbia. RESULTS--Among the 335 male fleet maintenance workers, the all cause standardised mortality ratio (SMR) was 0.50 (33 observed deaths, 95% confidence interval (95% CI) 0.35-0.70), and the all cancer SMR was 0.55 (nine deaths, 95% CI 0.25-1.05). Three deaths from lymphatic and haematopoietic cancer were observed; the SMR was 3.63 (95% CI 0.75-10.63). In the subgroup with highest potential for exposure to fuels and solvents, the SMR for leukaemia and aleukaemia was 9.26 (two deaths, 95% CI 1.12-33.43), and the SMR for other lymphatic and haematopoietic neoplasms was 2.57 (one death from malignant lymphoma, 95% CI 0.06-14.27). All three lymphatic and haematopoietic cancer deaths were among car and mobile equipment mechanics (one was an index case). The two additional index cases were a fourth mechanic who died of leukaemia in 1992, after mortality follow up ended, and a fifth mechanic who was diagnosed with leukaemia in 1988 and is still alive. CONCLUSION--Many garage mechanics in this cohort regularly used petrol to clean parts and to wash their hands; some workers would occasionally siphon petrol by mouth. Benzene, a recognised cause of haematological cancer, is a component of petrol. Previous research indicates that garage mechanics may be at risk of leukaemia and other haematological cancers, presumably due to exposure to petrol; this study supports those findings. PMID:7489058

  6. Decompression sickness in caisson workers

    PubMed Central

    Ghawabi, Samir H. El; Mansour, Mohamed B.; Youssef, Fatma L.; Ghawabi, Mohamed H. El; Latif, Mohamed M. Abd El

    1971-01-01

    El Ghawabi, S. H., Mansour, M. B., Youssef, F. L., El Ghawabi, M. H., and Abd El Latif, M. M. (1971).Brit. J. industr. Med.,28, 323-329. Decompression sickness in caisson workers. An investigation of 55 bridge construction workers is reported. The overall bends rate was 0·97%. (The term `bends' as used in this study is defined in the paper.) Chokes were encountered in 67·27% of workers. A clinical, haematological, and radiological study was performed. Definite bony changes were found in 43·6% of all workers; 91·6% of these had lesions around the elbow. The presence of dense areas in the neck of the scapula is reported in two cases for the first time. The relatively high haematocrit value is thought to play a part in the pathogenesis of bone infarction through its relation with blood viscosity. Images PMID:5124832

  7. Combining hypoxic methods for peak performance.

    PubMed

    Millet, Gregoire P; Roels, B; Schmitt, L; Woorons, X; Richalet, J P

    2010-01-01

    New methods and devices for pursuing performance enhancement through altitude training were developed in Scandinavia and the USA in the early 1990s. At present, several forms of hypoxic training and/or altitude exposure exist: traditional 'live high-train high' (LHTH), contemporary 'live high-train low' (LHTL), intermittent hypoxic exposure during rest (IHE) and intermittent hypoxic exposure during continuous session (IHT). Although substantial differences exist between these methods of hypoxic training and/or exposure, all have the same goal: to induce an improvement in athletic performance at sea level. They are also used for preparation for competition at altitude and/or for the acclimatization of mountaineers. The underlying mechanisms behind the effects of hypoxic training are widely debated. Although the popular view is that altitude training may lead to an increase in haematological capacity, this may not be the main, or the only, factor involved in the improvement of performance. Other central (such as ventilatory, haemodynamic or neural adaptation) or peripheral (such as muscle buffering capacity or economy) factors play an important role. LHTL was shown to be an efficient method. The optimal altitude for living high has been defined as being 2200-2500 m to provide an optimal erythropoietic effect and up to 3100 m for non-haematological parameters. The optimal duration at altitude appears to be 4 weeks for inducing accelerated erythropoiesis whereas <3 weeks (i.e. 18 days) are long enough for beneficial changes in economy, muscle buffering capacity, the hypoxic ventilatory response or Na(+)/K(+)-ATPase activity. One critical point is the daily dose of altitude. A natural altitude of 2500 m for 20-22 h/day (in fact, travelling down to the valley only for training) appears sufficient to increase erythropoiesis and improve sea-level performance. 'Longer is better' as regards haematological changes since additional benefits have been shown as hypoxic exposure increases beyond 16 h/day. The minimum daily dose for stimulating erythropoiesis seems to be 12 h/day. For non-haematological changes, the implementation of a much shorter duration of exposure seems possible. Athletes could take advantage of IHT, which seems more beneficial than IHE in performance enhancement. The intensity of hypoxic exercise might play a role on adaptations at the molecular level in skeletal muscle tissue. There is clear evidence that intense exercise at high altitude stimulates to a greater extent muscle adaptations for both aerobic and anaerobic exercises and limits the decrease in power. So although IHT induces no increase in VO(2max) due to the low 'altitude dose', improvement in athletic performance is likely to happen with high-intensity exercise (i.e. above the ventilatory threshold) due to an increase in mitochondrial efficiency and pH/lactate regulation. We propose a new combination of hypoxic method (which we suggest naming Living High-Training Low and High, interspersed; LHTLHi) combining LHTL (five nights at 3000 m and two nights at sea level) with training at sea level except for a few (2.3 per week) IHT sessions of supra-threshold training. This review also provides a rationale on how to combine the different hypoxic methods and suggests advances in both their implementation and their periodization during the yearly training programme of athletes competing in endurance, glycolytic or intermittent sports. PMID:20020784

  8. Pyoderma gangrenosum in myelodysplasia and acute leukaemia.

    PubMed Central

    Jacobs, P.; Palmer, S.; Gordon-Smith, E. C.

    1985-01-01

    Pyoderma gangrenosum is a rare occurrence in patients with haematological malignancy. This characteristic but nonspecific inflammatory process with skin destruction occurred in 4 patients with myelodysplasia, in one with acute leukaemic transformation of myelofibrosis, and in de novo acute myeloblastic leukaemia in another. Clinically, the cutaneous lesion in these patients differed from that associated with inflammatory bowel disease, arthritis, or the idiopathic type of pyoderma gangrenosum by having the vesiculo-bullous borders. Histopathological differences were also evident since more superficial layers of the skin were involved in the ulceration than typically encountered in patients with non-malignant systemic disease. Despite the less penetrating nature of this variant, treatment of the pyoderma gangrenosum is unsatisfactory and in the absence of effective therapy for the underlying disease, healing occurred only in the patient with acute leukaemia who achieved complete remission in response to chemotherapy. Images Figure 1 Figure 2 PMID:4034458

  9. Treatment of plasmablastic lymphoma with multiple organ involvement

    PubMed Central

    Liang, Rong; Wang, Zhe; Chen, Xie-qun; Bai, Qiang-xian

    2014-01-01

    We herein report the case of a 50-year-old woman who presented with persistent fever and a large mass in her right breast. Haematology, liver function, and other blood test results were abnormal. Computed tomography and positron emission tomography indicated that the lesion had spread to multiple organs. Immunohistochemical staining confirmed that the patient had plasmablastic lymphoma, an invasive and rare form of diffuse large B-cell lymphoma, and an underlying infection by the Epstein-Barr virus. After three rounds of CHOPE chemotherapy, followed by hyperCVAD and ESHAP, the patient achieved rapid and complete remission. This case is unusual in that the patient presented with a large breast mass and her recovery was extremely rapid. PMID:25630325

  10. The importance of cleanrooms for the treatment of haemato-oncological patients

    PubMed Central

    Matoušková, Ivanka

    2012-01-01

    The main purpose of cleanrooms in health care centres is to prevent hospital infections or leakage of a highly infectious agent (the source of haemorrhagic fevers, SARS, etc.) into the ambient environment and subsequently possibly threatening other individuals. Patients with haematological malignancies or after autologous or allogeneic haematopoietic stem cell transplantation (HSCT) rank among immunosuppressed individuals. Prolonged and deep neutropenia is considered a key risk factor of the occurrence of an exogenous infection. One of the possibilities of preventing an exogenous infection in these patients is to place them in a “cleanroom” for the crucial period of time. Cleanrooms are intensive care units with reverse isolation. The final part of the general article below provides an overview of the technology and types of cleanrooms for immunosuppressed patients in compliance with the current recommendations and technical standards. PMID:23788892

  11. Effects of oral ursodeoxycholic acid in healthy cats on clinicopathological parameters, serum bile acids and light microscopic and ultrastructural features of the liver.

    PubMed

    Nicholson, B T; Center, S A; Randolph, J F; Rowland, P J; Thompson, M B; Yeager, A E; Erb, H N; Corbett, J; Watrous, D

    1996-11-01

    A blind, placebo-controlled study evaluated the effects of ursodeoxycholic acid (UDCA) given orally, at a dose of 15 mg kg-1 per day for eight weeks, on the physical condition, haematological and serum biochemical profiles, urinalysis, total serum bile acids (TSBA) and hepatic histology of four healthy cats. There were no clinically important significant differences between the groups or within the treatment groups in clinicopathological parameters. TSBA concentrations or histology. A significant lower concentration/proportion of taurochenodeoxycholic acid was observed in the treated cats (P = 0.05). Only one treated cat accumulated measurable quantities of UDCA, and the compound appeared to be non-toxic. It did not increase the concentration of TSBA, and accumulated minimally in the serum. It should be investigated for therapeutic use in cats with hepatobiliary disease. PMID:8938858

  12. Advances in the Understanding of Cobalamin Assimilation and Metabolism

    PubMed Central

    Quadros, Edward V.

    2009-01-01

    Summary The haematological and neurological consequences of cobalamin deficiency define the essential role of this vitamin in key metabolic reactions. The identification of cubilin-amnionless as the receptors for intestinal absorption of intrinsic factor-bound cobalamin and the plasma membrane receptor for cellular uptake of transcobalamin bound cobalamin have provided a clearer understanding of the absorption and cellular uptake of this vitamin. As the genes involved in the intracellular processing of cobalamins and genetic defects of these pathways are identified, the metabolic disposition of cobalamins and the proteins involved are being recognized. The synthesis of methylcobalamin and 5’deoxyadenosylcobalamin, their utilization in conjunction with methionine synthase and methylmalonylCoA mutase, respectively, and the metabolic consequences of defects in these pathways could provide insights into the clinical presentation of cobalamin deficiency. PMID:19832808

  13. Molecular characterisation of Babesia gibsoni infection from a pit-bull terrier pup recently imported into South Africa.

    PubMed

    Matjila, P T; Penzhorn, B L; Leisewitz, A L; Bhoora, R; Barker, R

    2007-03-01

    Canine babesiosis caused by Babesia gibsoni was diagnosed in a 3-month-old Pit-bull pup during a routine clinical examination. Diagnosis was confirmed by way of smear examination, PCR, Reverse Line Blot (RLB) and sequence analysis which showed 100% homology with B. gibsoni (Japan AB118032) and Babesia sp. (Oklahoma) (AF205636). Haematology showed moderate anaemia and severe thrombocytopenia. Treatment was initiated with diminazene aceturate (Berenil RTU) followed by 2 doses of imidocarb diproprionate (Forray-65) 3 days and 14 days later, respectively. Babesia gibsoni DNA was still detectable 2 weeks post-treatment on the PCR/RLB test. A 10-day course of combination drug therapy using atovaquone and azithromycin was initiated. Blood samples taken on Day 1 and Day 40 after completion of treatment were negative for B. gibsoni DNA on PCR/RLB test. The implications of a possible introduction of B. gibsoni into South Africa are discussed. PMID:17665757

  14. Blood phagocyte activity after race training sessions in Thoroughbred and Arabian horses.

    PubMed

    Cywinska, Anna; Szarska, Ewa; Degorski, Andrzej; Guzera, Maciej; Gorecka, Renata; Strzelec, Katarzyna; Kowalik, Sylwester; Schollenberger, Antoni; Winnicka, Anna

    2013-10-01

    Intensive exercise and exertion during competition promote many changes that may result in the impairment of immunity and increased susceptibility to infections. The aim of this study was to evaluate the activity of "the first line of defense": neutrophils and monocytes in racing Thoroughbred and Arabian horses after routine training sessions. Twenty-three (12 Thoroughbred and 11 Arabian) horses were examined. Routine haematological (number of red blood cells - RBC, haemoglobin concentration - HGB, haematocrit - HCT, total number of white blood cells - WBC), biochemical (creatine phosphokinase activity - CPK and total protein concentration - TP) parameters, cortisol concentration as well as phagocytic and oxidative burst activity of neutrophils and monocytes were determined. The values of basic parameters and the activity of phagocytes differed between breeds and distinct patterns of exercise-induced changes were observed. The training sessions did not produce the decrease in phagocyte activity that might lead to the suppression of immunity. PMID:23664016

  15. Secondary Haemochromatosis in a Patient with Thalassemia Intermedia

    PubMed Central

    ROTARU, IONELA; GAMAN, AMELIA; GAMAN, G.

    2014-01-01

    Haemochromatosis is due to excessive accumulation of iron in tissues and organs impairing their function. The most common haematologic disorders that are subject to an intensive transfusion regimen bringing excess iron in the body are: thalassemia and myelodysplastic syndrome. The value of serum ferritin in these patients (indicator of iron stores condition) reaches high values. Red cell substitution bringing additional iron intake must be accompanied by administration of chelation therapy in order to prevent haemochromatosis and related complications. We present the case of a patient with thalassemia intermedia, integumentary secondary haemochromatosis, cirrhosis with haemochromatosis, and secondary diabetes, who died at the age of 33 years because of upper gastrointestinal bleeding due to the rupture of oesophageal varices. PMID:24791210

  16. Probable cutaneous hypersensitivity to carboplatin single-agent chemotherapy in a dog.

    PubMed

    Lanore, D; Sayag, D

    2010-12-01

    Carboplatin is usually a well-tolerated drug and has many applications in veterinary oncology. The side effects of carboplatin described in the veterinary literature include myelotoxicity, nephrotoxicity, digestive and appetite disorders. In 114 dogs treated by single-agent chemotherapy with carboplatin, we observed a rate of non-haematological toxicities of 19·3% (personal observation). This case report describes the first case of cutaneous delayed-hypersensitivity to carboplatin in a dog, diagnosed according to the official ABON-system, which determines a causal association between a suspected product and a reported reaction (A=probable, B=possible, O=unclassifiable and N=unlikely), and an experimental intradermal skin test. Antihistamines were used to treat the reaction, and future carboplatin treatments were adjusted by premedication with corticosteroids, prolonged infusion and a reduction of 20% of the first dose of carboplatin. No further reactions were noted during the following treatments. PMID:21121921

  17. Efficacy of Ficus spp. on renal injury induced by hypercholesterolaemia.

    PubMed

    Awad, Nagwa E; Hamed, Manal A; Seida, Ahmed A; Elbatanony, Marwa M

    2012-01-01

    The ethanol and hexane extracts of Ficus microcarpa, Ficus religiosa and Ficus mysorensis leaves were evaluated against renal injury induced by hypercholesterolaemia. Phytochemical screening of the investigated plants was undertaken. For the in vivo study, all rats were orally given cholesterol (30?mg?kg?ą body weight, BW) and leaves extract (500?mg?kg?ą BW) five times per week for 9 weeks. Hypercholesterolaemic rats showed significant increases in urea nitrogen and creatinine while serum protein and albumin levels, nitric oxide (NO), Na?-K?-ATPase and phospholipids in kidney tissue were all decreased. Treatment with leaves extract improved kidney function indices (urea nitrogen, creatinine, serum protein and albumin), kidney disorder biochemical parameters (NO, Na?-K?-ATPase and phospholipids), haematological profile (haemoglobin, RBCs and WBCs) and kidney histopathology. In conclusion, Ficus spp. succeeded in improving renal injury induced by hypercholesterolaemia, with the most potent effects seen while using Ficus microcarpa hexane extract. PMID:22432976

  18. Coagulopathy and placental abruption: changing management with ROTEM-guided fibrinogen concentrate therapy.

    PubMed

    McNamara, H; Mallaiah, S; Barclay, P; Chevannes, C; Bhalla, A

    2015-05-01

    Placental abruption may cause significant haemorrhage and coagulopathy that can progress rapidly due to simultaneous consumption and depletion of clotting factors. Plasma fibrinogen levels are predictive of further haemorrhage. Rapid detection and treatment of hypofibrinogenaemia is essential in the evolving clinical and haematological situation. The use of near-patient testing of coagulation using rotational thromboelastometry (ROTEM) allows dynamic monitoring of coagulopathy. Following the introduction of fibrinogen concentrate into our unit, a ROTEM-guided algorithm was developed for use in obstetric haemorrhage. We describe four cases of placental abruption, haemorrhage and severe coagulopathy that span the introduction of the algorithm. Three cases were associated with intrauterine death and the fourth with delivery of an extremely premature neonate. Rotational thromboelastometry was used in all cases but methods of fibrinogen replacement differ, illustrating evolving management of the condition in our unit. PMID:25659517

  19. Peripheral blood film - a review.

    PubMed

    Adewoyin, A S; Nwogoh, B

    2014-12-01

    The peripheral blood film (PBF) is a laboratory work-up that involves cytology of peripheral blood cells smeared on a slide. As basic as it is, PBF is invaluable in the characterization of various clinical diseases. This article highlights the basic science and art behind the PBF. It expounds its laboratory applications, clinical indications and interpretations in the light of various clinical diseases. Despite advances in haematology automation and application of molecular techniques, the PBF has remained a very important diagnostic test to the haematologist. A good quality smear, thorough examination and proper interpretation in line with patient's clinical state should be ensured by the haemato-pathologist. Clinicians should be abreast with its clinical utility and proper application of the reports in the management of patients. PMID:25960697

  20. Rapid screening for human-pathogenic Mucorales using rolling circle amplification.

    PubMed

    Dolatabadi, S; Najafzadeh, M J; de Hoog, G S

    2014-12-01

    Mucormycosis has emerged as a relatively common severe mycosis in patients with haematological and allogeneic stem cell transplantation. Source of transmission is from unidentified sources in the environment. Early diagnosis of infection and its source of contamination are paramount for rapid and appropriate therapy. In this study, rolling circle amplification (RCA) is introduced as a sensitive, specific and reproducible isothermal DNA amplification technique for rapid molecular identification of six of the most virulent species (Rhizopus microsporus, R. arrhizus var. arrhizus, R. arrhizus var. delemar, Mucor irregularis, Mucor circinelloides, Lichtheimia ramosa, Lichtheimia corymbifera). DNAs of target species were successfully amplified, with no cross reactivity between species. RCA can be considered as a rapid detection method with high specificity and sensitivity, suitable for large screening. PMID:25187214

  1. Rationale and efficacy of CD52 targeting in HTLV-1-associated myositis.

    PubMed

    Cochereau, Delphine; Georgin-Lavialle, Sophie; Maisonobe, Thierry; Dubourg, Odile; Melboucy-Belkhir, Sara; Hermine, Olivier; Aouba, Achille

    2014-07-01

    We retrospectively analysed two selected patients, referred to our Haematology Department for refractory HTLV-1 associated myositis with circulating pathologic T-cell population with ATL phenotype. They respectively presented also HTLV-1 associated Crohn-like disease and myelopathy. Muscle biopsy of both patients was analysed to determine the pathologic infiltrate. Alemtuzumab was proposed as salvage therapy. Targeting CD52 with alemtuzumab showed good efficacy on myopathy of both patients for respectively 11 and 10 months. Interestingly, this treatment showed also efficacy on circulating pathologic T-cell population and on concomitant digestive and neurological diseases. The double infected cells ablation and immunosuppressive propriety of alemtuzumab probably explains its interest in this infectious and dysimmunitary disorder. Even though alemtuzumab probably remains a suspensive treatment, its place should be assessed in controlled trial in this difficult to treat rare disease. PMID:24709000

  2. Novel Cytogenetic Aberrations in a Patient of Chronic Myeloid Leukemia with Blast Crisis

    PubMed Central

    Shah, Bhoumik; Gajendra, Smeeta; Sharma, Atul

    2015-01-01

    Chronic myeloid leukaemia (CML) is a clonal haematological disease which is characterized by a diagnostic karyotypic abnormality t (9;22)(q34;q11) called as Philadelphia (Ph) chromosome. Occurrence of additional chromosomal abnormalities besides the Ph chromosome is defined as clonal evolution (CE) and considered to be a marker of disease progression. A 67-year-old male who was initially evaluated at a private hospital where a diagnosis of acute promyelocytic leukaemia was made on bone marrow aspirate with ambiguous RT-PCR report referred to our centre for further evaluation and treatment. On conventional karyotyping, Ph chromosome along with translocations t(5;13)(q12;p13), t(15;20)(q22;p13) and monosomy 13 was observed in all 20 metaphases. A final diagnosis of CML-myeloid blast crisis with complex cytogenetics was made. Patient succumbed to death within one month of initiation of imatinib therapy.

  3. Pharmacological targets in the ubiquitin system offer new ways of treating cancer, neurodegenerative disorders and infectious diseases

    PubMed Central

    Edelmann, Mariola J.; Nicholson, Benjamin; Kessler, Benedikt M.

    2011-01-01

    Recent advances in the development and discovery of pharmacological interventions within the ubiquitin–proteasome system (UPS) have uncovered an enormous potential for possible novel treatments of neurodegenerative disease, cancer, immunological disorder and microbial infection. Interference with proteasome activity, although initially considered unlikely to be exploitable clinically, has already proved to be very effective against haematological malignancies, and more specific derivatives that target subsets of proteasomes are emerging. Recent small-molecule screens have revealed inhibitors against ubiquitin-conjugating and -deconjugating enzymes, many of which have been evaluated for their potential use as therapeutics, either as single agents or in synergy with other drugs. Here, we discuss recent advances in the characterisation of novel UPS modulators (in particular, inhibitors of ubiquitin-conjugating and -deconjugating enzymes) and how they pave the way towards new therapeutic approaches for the treatment of proteotoxic disease, cancer and microbial infection. PMID:22088887

  4. Piroplasm parasites of white rhinoceroses (Ceratotherium simum) in the Kruger National Park, and their relation to anaemia.

    PubMed

    Govender, D; Oosthuizen, M C; Oosthuisen, M C; Penzhorn, B L

    2011-03-01

    As part of a larger survey to map the geographical distribution of Babesia and Theileria parasites in the southern African rhinoceros population, white rhinoceroses were sampled during routine immobilisations in the Kruger National Park. Polymerase chain reaction (PCR) and reverse line blot (RLB) hybridisation assays were used to screen for the presence of piroplasms and complete blood counts were used to assess associated changes in clinical parameters. Of the 195 rhinoceroses sampled, 71 (36.4 %) tested positive for the presence of Theileria bicornis, with no significant change in the haematological parameters measured, while 18 (9.2 %) tested positive for Theileria equi. None of the rhinoceroses sampled tested positive for Babesia bicornis, a parasite associated with mortalities in black rhinoceroses. PMID:21826836

  5. Virtual anthropology and forensic identification using multidetector CT

    PubMed Central

    Savall, F; Mokrane, F-Z; Rousseau, H; Crubézy, E; Rougé, D; Telmon, N

    2014-01-01

    Virtual anthropology is made possible by modern cross-sectional imaging. Multislice CT (MSCT) can be used for comparative bone and dental identification, reconstructive identification and lesion identification. Comparative identification, the comparison of ante- and post-mortem imaging data, can be performed on both teeth and bones. Reconstructive identification, a considerable challenge for the radiologist, identifies the deceased by determining sex, geographical origin, stature and age at death. Lesion identification combines virtual autopsy and virtual anthropology. MSCT can be useful in palaeopathology, seeking arthropathy, infection, oral pathology, trauma, tumours, haematological disorders, stress indicators or occupational stress in bones and teeth. We examine some of the possibilities offered by this new radiological subspeciality that adds a new dimension to the work of the forensic radiologist. A multidisciplinary approach is crucial and involves communication and data exchange between radiologists, forensic pathologists, anthropologists and radiographers. PMID:24234584

  6. Multiple myeloma.

    PubMed Central

    MacLennan, I. C.; Drayson, M.; Dunn, J.

    1994-01-01

    Multiple myeloma occurs in over 2000 new patients in England and Wales each year. It presents most frequently as bone pain and patients tend to become dehydrated and may develop renal failure. No available treatment is curative, but about two thirds of patients achieve a stable response with low dose combination chemotherapy. Combination chemotherapy including doxorubicin and carmustine with the alkylating agents cyclophosphamide and melphalan achieve a higher stable response rate than conventional treatment with melphalan and prednisone without additional haematological toxicity. These responses are associated with loss of bone pain and patients remain symptom free for months without further treatment. Relapse occurs on average in a little under two years and, though second responses are frequently obtained, the disease eventually becomes refractory. This paper looks at who should be treated and the benefits that may be expected from the treatments available. PMID:8068084

  7. Review of retrospective dosimetry techniques for external ionising radiation exposures.

    PubMed

    Ainsbury, E A; Bakhanova, E; Barquinero, J F; Brai, M; Chumak, V; Correcher, V; Darroudi, F; Fattibene, P; Gruel, G; Guclu, I; Horn, S; Jaworska, A; Kulka, U; Lindholm, C; Lloyd, D; Longo, A; Marrale, M; Monteiro Gil, O; Oestreicher, U; Pajic, J; Rakic, B; Romm, H; Trompier, F; Veronese, I; Voisin, P; Vral, A; Whitehouse, C A; Wieser, A; Woda, C; Wojcik, A; Rothkamm, K

    2011-11-01

    The current focus on networking and mutual assistance in the management of radiation accidents or incidents has demonstrated the importance of a joined-up approach in physical and biological dosimetry. To this end, the European Radiation Dosimetry Working Group 10 on 'Retrospective Dosimetry' has been set up by individuals from a wide range of disciplines across Europe. Here, established and emerging dosimetry methods are reviewed, which can be used immediately and retrospectively following external ionising radiation exposure. Endpoints and assays include dicentrics, translocations, premature chromosome condensation, micronuclei, somatic mutations, gene expression, electron paramagnetic resonance, thermoluminescence, optically stimulated luminescence, neutron activation, haematology, protein biomarkers and analytical dose reconstruction. Individual characteristics of these techniques, their limitations and potential for further development are reviewed, and their usefulness in specific exposure scenarios is discussed. Whilst no single technique fulfils the criteria of an ideal dosemeter, an integrated approach using multiple techniques tailored to the exposure scenario can cover most requirements. PMID:21183550

  8. Subclinical diseases underlying poor performance in endurance horses: diagnostic methods and predictive tests.

    PubMed

    Fraipont, A; Van Erck, E; Ramery, E; Richard, E; Denoix, J-M; Lekeux, P; Art, T

    2011-08-01

    Thirty-eight endurance horses underwent clinical and ancillary examinations, including haematological and biochemical evaluation, standardised exercise tests both on a treadmill and in the field, Doppler echocardiography, impulse oscillometry, video endoscopy and collection of respiratory fluids. All of the examined poorly performing horses were affected by subclinical diseases, and most of them had multiple concomitant disorders. On the contrary, the well-performing horses were free of any subclinical disease. The most frequently diagnosed diseases were respiratory disorders, followed by musculoskeletal and cardiac problems. Poor performers exhibited lower speeds at blood lactate concentration of 4 mmol/l (VLA4) and at heart rates of 160 (V160) and 200 bpm (V200) on the treadmill and in the field, as well as slower recovery of heart rate. PMID:21795311

  9. A case of cranial multinevritis: from the onset to the diagnosis of primary neurolymphomatosis

    PubMed Central

    Vecchio, Domizia; Mittino, Daniela; Terazzi, Emanuela; Luca, Nassi; Conconi, Annarita; Monaco, Francesco

    2012-01-01

    Neurolymphomatosis (NL) is a rare peripheral or cranial neuropathy caused by non-Hodgkin’s lymphoma (NHL). Diagnosis is often delayed and prognosis is poor. The authors described a woman in her 70s with a facial left peripheral palsy, complete right abducent palsy, left hypoacusia and balance deficit. Then she presented with low progressive hyposthenia at four limbs and cognitive impairment, sudden facial right peripheral palsy and complete left abducent palsy. The authors performed brain and spinal MRI, cerebrospinal fluid (CSF) analysis and extensive haematological examinations for infections, autoimmune and neoplastic diseases. All the results were not diagnostic. Only repeating for the third time a spinal tap, CSF presented neoplastic B cells suggestive for large B-NHL. The authors diagnosed primary NL. The patient was treated with R-CHOP but she died 2 months later. In front of rapidly progressive neuropathy, a NL has to be considered performing different examinations, especially and repeating them after a short period. PMID:22707692

  10. ICSH recommendations for the standardization of nomenclature and grading of peripheral blood cell morphological features.

    PubMed

    Palmer, L; Briggs, C; McFadden, S; Zini, G; Burthem, J; Rozenberg, G; Proytcheva, M; Machin, S J

    2015-06-01

    These guidelines provide information on how to reliably and consistently report abnormal red blood cells, white blood cells and platelets using manual microscopy. Grading of abnormal cells, nomenclature and a brief description of the cells are provided. It is important that all countries in the world use consistent reporting of blood cells. An international group of morphology experts have decided on these guidelines using consensus opinion. For some red blood cell abnormalities, it was decided that parameters produced by the automated haematology analyser might be more accurate and less subjective than grading using microscopy or automated image analysis and laboratories might like to investigate this further. A link is provided to show examples of many of the cells discussed in this guideline. PMID:25728865

  11. [What is the role of other complementary examination in amyotrophic lateral sclerosis?].

    PubMed

    Tranchant, C

    2006-06-01

    Amotrophic lateral sclerosis diagnosis is based on clinical and electrophysiological findings. Transcranial magnetic stimulation and MRI can show abnormalities which are not specific, but which can confirm upper motor neuron involvement. The other tests are performed to exclude differential diagnosis. Tests which should be performed in every cases are: medullar MRI, blood counts, erythrocyte sedimentation, serum protein electrophoresis, calcium, phosphore, serological tests for HIV, siphylis, Lyme disease. Other tests are made in some clinical circonstances to exclude genetical disease or metabolic disorders (SMN gene, Kennedy gene, Hexosaminidase A, very long chaine fatty acids), haematological or paraneoplasic disorders (anti-neurons antibodies, PSA, CT of chest and abdomen, mammography, bone marrow biopsy) or inclusion myositis (muscle biopsy). PMID:17128090

  12. Tolerance studies with brotizolam in hospitalized patients

    PubMed Central

    von Delbrück, Orla; Goetzke, Edda; Nagel, Cornelia

    1983-01-01

    1 A long-term study of brotizolam (minimum 4 weeks: maximum 26 weeks) was carried out in hospitalized patients (29 to 95 years) who complained of sleep disturbance. 3.0% of the patients used 0.125 mg, 86.4% used 0.25 mg, and 10.0% used 0.5 mg daily. During the trial there was no evidence of tolerance. 2 There were no symptoms of overdosage, physical and psychological dependency or withdrawal, and there were no interactions with the concurrently prescribed drugs. 3 There were no changes in vital functions, haematology, or in the biochemical investigations of blood or urine which could be attributed to the drug. PMID:6362697

  13. Human parvovirus B19 infection within a family and risk for pregnant women.

    PubMed Central

    Azzi, A.; Trotta, M.; Zakrzewska, K.; Balzer, E.; Rogasi, P. G.; Carocci, A.; Leoncini, F.

    1996-01-01

    During an outbreak of parvovirus B19 infection among four related families at least 70% of the household contacts, including a woman at the 33rd week of pregnancy, became infected. Twins were born at the 39th week of pregnancy, both with B19 infection. B19 DNA was detected in their sera by a nested PCR, anti-B19 IgM was detectable only by an immunofluorescence assay, and low levels of maternal anti-B19 IgG were demonstrable by an immunoenzymatic test in the serum of both children. All the haematological parameters were normal at birth and 6 months later, when B19 DNA and anti-B19 antibody were no longer detectable in serum samples. This observation emphasizes the high risk of B19 infection among household contacts and the possibility of a favourable outcome of the foetal infection, possibly related to infection late in the pregnancy. PMID:8870639

  14. Is it time to revisit contraindications to organ donation from donors with a JAK-2 mutation? Safe use of a liver allograft from a donor with essential thrombocythaemia.

    PubMed

    Haldar, Debashis; Chen, Frederick; Byron, Jane; Elsharkawy, Ahmed Mohamed; Perera, M Thamara Pr

    2015-07-01

    Transplantation can cure end-stage liver disease and hepatocellular carcinoma. However, the balance of organ demand and provision is heavily tipped to the detriment of patients. Patients awaiting transplantation rely on the greater use of marginal donors that may carry a risk to the recipient. UK authorities have decreed donor haematological malignancy an absolute contraindication. The authors describe the first report of a patient being safely transplanted with a liver from a donor who suffered from JAK2 V617F mutation-driven essential thrombocythaemia to a patient with a critical burden of hepatocellular carcinoma. A year after transplantation, the patient has neither evidence of acquisition of the donor's pathology, nor evidence of carcinoma recurrence. The case highlights the responsibility of the recipient team to maximize the use of organs by expert risk assessment. Dissemination of experience should inform future decisions, benefit patients and bolster utility in an era of growing waiting-list mortality. PMID:25778631

  15. Retinopathy in chronic hepatitis C patients during interferon treatment with ribavirin

    PubMed Central

    Jain, K; Lam, W; Waheeb, S; Thai, Q; Heathcote, J

    2001-01-01

    AIM—To assess the ocular effect of interferon alfa 2b prescribed with ribavirin in patients undergoing therapy for chronic hepatitis C.?METHODS—19 patients with chronic hepatitis C who satisfied the follow up criteria were assessed for ocular complications using slit lamp biomicroscopy and indirect ophthalmoscopy before, during, and after the treatment at regular intervals.?RESULTS—8/19 patients, while on treatment, developed an asymptomatic retinopathy. Among these 3/8 were relapsers and 5/9 were non-responders to interferon monotherapy. All retinal changes faded, often while the patients continued the therapy. There was no significant association in occurrence of retinopathy with haematological and/or biochemical changes.?CONCLUSION—Retinopathy was more common in interferon monotherapy non-responders than relapsers when treated with interferon alfa 2b with the addition of ribavirin. The changes were transient, disappearing while the patients were still being treated.?? PMID:11567959

  16. Exposure of workers to a mixture of toluene and xylenes. II. Effects.

    PubMed Central

    Chen, Z; Liu, S J; Cai, S X; Yao, Y M; Yin, H; Ukai, H; Uchida, Y; Nakatsuka, H; Watanabe, T; Ikeda, M

    1994-01-01

    The health effects of exposure to a mixture of toluene and xylene isomers was studied on the fourth or fifth days of a working week in factories in China. The study population comprised 233 subjects (122 men and 111 women), who were exposed to the time weighted geometric mean (maximum) concentrations of toluene (3 (203) ppm) and xylenes (4 (103) ppm). For comparison, 241 non-exposed controls (116 men and 125 women) were recruited from the same regions. The prevalence of some subjective symptoms significantly increased in the exposed population, and the symptom profiles were similar to those found after exposure to toluene or xylenes alone. Haematology and serum biochemistry did not show notable changes. It seems reasonable to conclude that the effects of the toxicities of toluene and xylenes in combination are additive. PMID:8124463

  17. Filamentous fungi infection in patients with myelodysplastic syndrome. A report of twelve cases.

    PubMed

    Mele, Luca; Ricci, Paolo; Nosari, Annamaria; Tonso, Anna; Fianchi, Luana; Cudillo, Laura; Pagano, Livio

    2002-07-01

    In this report we analyse the risk factors, the clinical characteristics and outcome of patients with myelodysplastic syndrome (MDS) who developed an Invasive Fungi Infection (IFI). This was a multicentric study involving 14 Italian Haematological Divisions during a 10-year period whose object was to identify the characteristics of patients with this infection. The study recorded 391 consecutive documented IFI, 12 of which (3%) occurred in MDS patients, from 5 of the participating centres. The primary localization of infection was lung in 10 cases and skin and paranasal sinus in 1 case each. Ten patients died at the end of follow up. The death was mainly attributable to IFI progression in nine of them. The factors that appeared related to an unfavourable outcome were intensive chemotherapy within 30 days before IFI diagnosis, presence of multiple localization at chest X-ray in patients with isolated pulmonary IFI and multiple sites of infection. PMID:12389623

  18. Invasive fungal infection in patients with myelodysplastic syndrome: a report of twelve cases.

    PubMed

    Mele, Luca; Ricci, Paolo; Nosari, Annamaria; Tonso, Anna; Fianci, Luana; Cudillo, Laura; Pagano, Livio

    2002-08-01

    In this report we analyse the risk factors, clinical characteristics and outcome of patients with myelodysplastic syndrome (MDS) who developed a invasive fungal infection (IFI). This was a multicentric study involving 14 Italian Haematological Divisions during a 10-year-period whose object was to identify the characteristics of patients with this infection. The study recorded 391 consecutive documented IF, 12 of which (3%) occurred in MDS patients from five of the participating centres. The primary localisation of infection was the lung in 10 cases and skin and paranasal sinus in one case each. Ten patients died at the end of the follow up. The death was mainly attributable to IFI progression in nine of them. The factors which appeared related to an unfavourable outcome were intensive chemotherapy within 30 days before IFI diagnosis, presence of multiple localisation at chest X-ray in patients with isolated pulmonary IFI and multiple sites of infection. PMID:12400604

  19. New diagnostic possibilities in systemic neonatal infections: metabolomics.

    PubMed

    Dessě, Angelica; Corsello, Giovanni; Stronati, Mauro; Gazzolo, Diego; Caboni, Pierluigi; Carboni, Roberta; Fanos, Vassilios

    2014-03-01

    Systemic neonatal infection is a serious complication in preterm and term infants and is defined as a complex clinical syndrome caused by bacteria, fungi and virus. Sepsis remains among the leading causes of death in both developed and underdeveloped countries above all in the neonatal period. Earlier diagnosis may offer the ability to initiate treatment to prevent adverse outcomes. There have been many studies on various diagnostic haematological markers like acute phase reactants, C-reactive protein, procalcitonin, interleukins and presepsin. However, there is still no single test that satisfies the criteria as being the ideal marker for the early diagnosis of neonatal sepsis. In this regard, metabolomic analysis seems to be a promising method for determining metabolic variations correlated with systemic neonatal infections. PMID:24709449

  20. 46,XY hypergonadotropic hypogonadism and myasthenia gravis.

    PubMed

    Lichiardopol, Corina; Herlea, V; Ioan, Virginia; Tomulescu, V; Mixich, F

    2006-01-01

    Both hypergonadotropic hypogonadism and myasthenia gravis can be parts of type II autoimmune polyendocrine syndrome and association between the two disorders has been reported in few cases. A 14 year old male patient with a personal history of bilateral cryptorchidism and ptosis was referred for delayed puberty. Clinical examination revealed eunuchoid habitus, small, soft testes, gynecomastia, ptosis, a myasthenic deficit score of 22.5 points and an IQ of 84 points. Decreased testosterone (0.064 ng/mL) and elevated LH (64.5 mUI/mL) were consistent with hypergonadotropic hypogonadism and karyotype was normal: 46,XY. Thyroid function, haematologic evaluation, BUN, electrolytes, and glycemia were in the normal range. Therapy consisted of anticholinesterase inhibitors, immunosuppressants, corticotherapy, testosterone; thoracoscopic thymectomy was performed showing thymic lymphoid hyperplasia on histopathologic examination. Myasthenic score improved (12.5 points), progressive virilization occurred, and a year later the patient presented with cushingoid features and obesity. PMID:17308692

  1. WITH: a system to write clinical trials using XML and RDBMS.

    PubMed Central

    Fazi, Paola; Luzi, Daniela; Manco, Mariarosaria; Ricci, Fabrizio L.; Toffoli, Giovanni; Vignetti, Marco

    2002-01-01

    The paper illustrates the system WITH (Write on Internet clinical Trials in Haematology) which supports the writing of a clinical trial (CT) document. The requirements of this system have been defined analysing the writing process of a CT and then modelling the content of its sections together with their logical and temporal relationships. The system WITH allows: a) editing the document text; b) re-using the text; and c) facilitating the cooperation and the collaborative writing. It is based on XML mark-up language, and on a RDBMS. This choice guarantees: a) process standardisation; b) process management; c) efficient delivery of information-based tasks; and d) explicit focus on process design. PMID:12463823

  2. Heinz body haemolytic anaemia in a dog secondary to ingestion of a zinc toy: a case report.

    PubMed

    Bexfield, Nicholas; Archer, Joy; Herrtage, Michael

    2007-09-01

    A 6-year-old Labrador retriever was referred for investigation of severe lethargy and suspected immune-mediated haemolytic anaemia. Clinical examination revealed pale mucous membranes and jaundice. Haematology demonstrated large numbers of Heinz bodies and a marked anaemia, which was strongly regenerative. Serum zinc concentrations were markedly elevated. Analysis of a metal toy vomited by the dog 3 days prior to presentation revealed it to be composed of almost pure zinc. A diagnosis of haemolytic anaemia secondary to acute zinc toxicity was made and supportive therapy instigated. There was a subsequent decrease in numbers of Heinz bodies and a rise in the haematocrit, and the dog made an uneventful recovery. Acute zinc toxicity resulting in haemolytic anaemia is rarely observed, and this case was also unusual in that the main clinicopathological finding was the presence of numerous Heinz bodies without other evidence of oxidative damage to red blood cells. PMID:17307371

  3. A Case of Fulminant Hepatitis due to Echovirus 9 in a Patient on Maintenance Rituximab Therapy for Follicular Lymphoma

    PubMed Central

    Thomson, S. J.; Legg, Joanne; Narat, Santosh

    2015-01-01

    Rituximab is a CD20 monoclonal antibody commonly used in the treatment of haematological malignancies. It causes lymphopenia with subsequent compromised humoral immunity resulting in an increased risk of infection. A number of infections and viral reactivations have been described as complicating Rituximab therapy. We report an apparently unique case of echovirus 9 (an enterovirus) infection causing an acute hepatitis and significant morbidity in an adult patient on maintenance treatment of Rituximab for follicular lymphoma. We also describe potential missed opportunities to employ more robust screening for viral infections which may have prevented delays in the appropriate treatment and thus may have altered the patient's clinical course. We also make suggestions for lowering the threshold of viral testing in similar patients in the future. PMID:26106492

  4. Numb Chin Syndrome as First Symptom of Diffuse Large B-Cell Lymphoma

    PubMed Central

    Carbone, Mario; Della Ferrera, Francesco; Carbone, Lucio; Gatti, Gaia; Carrozzo, Marco

    2014-01-01

    Numb chin syndrome is a rare sensory neuropathy of the mental nerve characterized by numbness, hypoesthesia, paraesthesia, and very rarely pain. Dental causes, especially iatrogenic ones, maxillofacial trauma, or malignant neoplasm are etiologic factors for this rare syndrome. Many malignant and metastatic neoplasms are causing this syndrome, like primary osteosarcoma, squamous cell carcinoma, and mandibular metastasis of primary carcinoma of breast, lung, thyroid, kidney, prostate, and nasopharynx. Haematological malignancies like acute lymphocytic leukaemia, Hodgkin and non-Hodgkin lymphoma, and myeloma can cause this neuropathy. The authors report a case of a 71-year-old woman in which the numb chin syndrome was the first symptom of the diffuse large B-cell lymphoma, which caused infiltration and reabsorption of the alveolar ridge and lower mandibular cortex. A biopsy of the mass was performed on fragments of tissue collected from the mandibular periosteum, medullary and cortical mandibular bone, and inferior alveolar nerve. PMID:25580308

  5. Idelalisib: first global approval.

    PubMed

    Markham, Anthony

    2014-09-01

    Idelalisib (Zydelig(®)) is a highly specific small-molecule phosphatidylinositol-3-kinase (PI3K?) inhibitor that has been developed as an oral treatment for B cell haematological cancers.It has received its first approval in the US in July 2014 for the treatment of relapsed chronic lymphocytic leukaemia(CLL), relapsed follicular B-cell non-Hodgkin lymphoma(NHL) and relapsed small lymphocytic lymphoma (SLL) [corrected]. Idelalisib is under regulatory review in the EU-where it has received a positive opinion from the European Medicines Agency Committee for Medicinal Products for Human Use-and in clinical development for CLL in Australia and Canada. This article summarizes the milestones in the development of Idelalisib leading to this first approval for relapsed CLL, NHL and SLL. PMID:25187123

  6. No evidence or no alternative? Taking responsibility for off-label prescribing.

    PubMed

    Ghinea, N; Lipworth, W; Kerridge, I; Day, R

    2012-03-01

    Recombinant activated factor VII (rFVIIa) is registered for patients with rare haematological disorders, but is used 'off-label' in many other situations, including intracranial haemorrhage, cardiac surgery, trauma, transplantation and prostatectomy. Lack of systematic evidence to support these off-label uses has not slowed the growth of off-label prescribing of rFVIIa. We use the case of rFVIIa to illustrate the issues raised by off-label prescribing, and the kind of impasse that can arise when views about evidence, expertise and clinical necessity are in conflict. We argue that clinicians, hospital drug committees and regulators all need to acknowledge the complexity of prescribing decisions, and ensure that decisions to prescribe off-label are sufficiently justified. PMID:22432984

  7. Raised concentration of plasma creatine kinase BB isoenzyme in myelodysplasia.

    PubMed Central

    Crook, M; Williams, A; Sankaralingam, A; Tutt, P

    1994-01-01

    A 72 year old woman presented with a suspected myocardial infarction. An echocardiograph showed no acute changes but her plasma creatine kinase (CK) activity was increased at 343 U/l (< 175 normal range). The apparent creatine kinase-MB activity by a CK-M subunit immunoinhibition assay was 350 U/l. In view of the discrepancy between the total creatine kinase and CK-MB activity plasma creatine kinase electrophoresis studies were performed which showed not only a band of creatine kinase-MM but also a band of creatine kinase-BB, 53% of the total creatine kinase activity. No band of CK-MB was seen. It later transpired that the woman had myelodysplasia. It is suggested that premalignant and malignant haematological conditions should be considered in patients with an unexplained increase in plasma CK-BB. PMID:8063940

  8. Hepatitis viruses and non-Hodgkin’s lymphoma: A review

    PubMed Central

    Datta, Sibnarayan; Chatterjee, Soumya; Policegoudra, Rudragoud S; Gogoi, Hemant K; Singh, Lokendra

    2012-01-01

    Non-Hodgkin’s lymphoma (NHL) is among the haematological malignancies with high prevalence worldwide, causing estimated 355?900 new cases and 191?400 deaths in 2008. High prevalence of NHL is documented in economically more developed areas while low prevalence is observed in less developed areas of the globe. A wide array of environmental factors have been reported to be either directly involved or in modifying the risk of NHL development. In addition to these factors, a number of infectious agents, chiefly viruses have also been implicated in the development of NHL. This article reviews the available literature to discuss the role of hepatitis viruses in NHL development, possible mechanisms of lymphomagenesis and also identify the areas in which further research is required to better understand this disease. A brief discussion on the clinical aspects such as classification, staging, treatment approaches have also been included in this article. PMID:24175222

  9. Antemortem diagnosis with multiple random skin biopsies and transbronchial lung biopsy in a patient with intravascular large B-cell lymphoma, the so-called Asian variant lymphoma.

    PubMed

    Nishizawa, Tomotaka; Saraya, Takeshi; Ishii, Haruyuki; Goto, Hajime

    2014-01-01

    A 59-year-old, previously healthy man presented to our hospital, with a 3-month history of high fever, nocturnal sweating and exertional dyspnoea. Aggressive diagnostic procedures such as multiple random skin biopsies and transbronchial lung biopsy (TBLB) led to an antemortem diagnosis of intravascular large B-cell lymphoma (IVLBCL), which showed abundant CD20 atypical lymphocytes aggregated in lumina of small vessels. The 29 cases diagnosed with IVLBCL during their lifetime by TBLB were reviewed. Their clinical features included respiratory symptoms (hypoxaemia, dyspnoea and dry cough) and persistent fever. IVLBCL patients show various radiological patterns (ground glass opacities, multiple centrilobular nodules, interlobular septal thickening, interstitial shadows and thickening of bronchovascular bundles), suggesting lymphatic or haematological spread. Antemortem diagnosis of IVLBCL is difficult, but a multidisciplinary approach, with aggressive multiple random skin biopsies and/or TBLB, should be considered in patients with respiratory symptoms that are refractory to antibiotics or prednisolone treatment. PMID:24632902

  10. Evaluation of Erythrocytes, Platelets, and Serum Iron Profile in Dogs with Chronic Enteropathy

    PubMed Central

    Marchetti, Veronica; Lubas, George; Lombardo, Andrea; Corazza, Michele; Guidi, Grazia; Cardini, Giovanni

    2010-01-01

    The aim of this study is to evaluate iron status, erythrocyte, and platelet modifications in dogs with chronic enteropathy (CE). Dogs were grouped as food-responsive diarrhea (FRD, n = 11), antibiotic-responsive diarrhea (ARD, n = 5), and steroid-responsive diarrhea (SRD, n = 6) relating to therapeutic-response. Clinical and haematological findings, evidence of gastrointestinal blood loss, and iron metabolism were evaluated before and after treatment. A mild normocytic or microcytic anemia and thrombocytosis were identified, respectively in 18.0% and 31.8% of CE dogs. No significant differences between pre- and posttreatment of hematocrit, haemoglobin, and mean corpuscular volume, platelet count and mean platelet volume were found. Statistical analysis pointed out significant differences between pre- and posttreatment in serum iron (P < .03) and unsaturated iron binding capacity (UIBC) (P < .01). No significant correlations were found between these parameters and canine Inflammatory Bowel Disease activity index and pattern of CE as well. PMID:20798868

  11. [Effectiveness and disposition of the newly developed cephalosporin cefquinome in puerperal septicemia and toxemia in gilts].

    PubMed

    Heinritzi, K; Hagn, J

    1999-04-01

    Epizootiological, clinical, bacteriological and haematological studies were carried out to assess the effectiveness of the recently developed cephalosporin preparation Cefquinome in the treatment of the puerperal septicaemia and toxaemia syndrome. Cefquinome was administered at three different doses (1, 2 and 4 mg/kg BW) to 188 sows with feverish puerperal illness. Amoxicillin (7 mg/kg BW) was used as a control drug. In 41% of cases endometritis was a monoinfection whereas in 70% of mammary infections mixed infections were diagnosed. Results showed that for therapy of puerperal septicaemia and toxaemia Cefquinome at doses of 2 mg/kg BW and 4 mg/kg BW is clearly more effective than the control drug Amoxicillin and Cefquinome at its lowest dose of 1 mg/kg BW. PMID:10326238

  12. A phase II study of paclitaxel in heavily pretreated patients with small-cell lung cancer.

    PubMed Central

    Smit, E. F.; Fokkema, E.; Biesma, B.; Groen, H. J.; Snoek, W.; Postmus, P. E.

    1998-01-01

    The purpose of the study was to delineate the efficacy and toxicity of paclitaxel (Taxol, Bristol Myers Squibb) in the treatment of drug resistant small-cell lung cancer (SCLC). Patients with SCLC relapsing within 3 months of cytotoxic therapy received paclitaxel 175 mg m(-2) intravenously over 3 h every 3 weeks. The dose of paclitaxel was adjusted to the toxicity encountered in the previous cycle. Of 24 patients entered into the study, 24 and 21 were assessable for response and toxicity respectively. There were two early deaths and two toxic deaths. No complete and seven partial responses (29%) (95%CI 12-51%) were observed and five patients had disease stabilization. The median survival (n = 21) was 100 days. Life-threatening toxicity occurred in four patients; in others (non)-haematological toxicity was manageable. Paclitaxel is active in drug-resistant SCLC. Further investigation in combination with other active agents in this poor prognosis group is appropriate. PMID:9461009

  13. The myelodysplastic syndromes: the era of understanding.

    PubMed

    Meers, Stef

    2015-05-01

    Myelodysplastic syndromes are a heterogeneous group of clonal haematological stem cell disorders. Allogeneic stem cells transplantation remains the only curative treatment but only a minority of patients are eligible for this treatment. In spite of this, it has become clear that treatment with lenalidomide and azanucleotides can lead to increased overall survival in particular subsets of patients with MDS. The relative silence on the therapeutic side is counter-balanced by major advances in the understanding of this heterogeneous disease. The introduction of high-throughput molecular techniques has resulted in the discovery that most patients harbour molecular aberrations, including pathways such as the spliceosome machinery previously not known to be involved. These newly discovered pathways will undoubtedly result in new therapeutic strategies for this difficult to treat disease. PMID:25186093

  14. [A literature review of equine piroplasmosis after an episode of acute babesiosis in a Dutch Standardbred foal after a stay in Normandy].

    PubMed

    Butler, C M; van Gils, J A M; van der Kolk, J H

    2005-12-01

    Piroplasmosis, a disease endemic to most tropical and subtropical areas, appears to be spreading to more temperate zones. This article gives a review of equine piroplasmosis and describes an acute case of infection with Babesia caballi in a Dutch Standard bred foal after a short stay at a stud in Normandy (France). A 3-month-old stallion foal was presented with lethargy, fever of 41 degrees C, and pale mucosal membranes. Haematology revealed a low packed cell volume (14 l/l) leucytosis (25 G/l) and a high blood urea nitrogen concentration (20.1mmol/l). Infection with B. caballi was diagnosed on the basis of Giemsa staining blood smears and was confirmed by polymerase chain reaction in combination with RLB. Treatment with imidocarb dipropionate and a blood transfusion resolved the haemolytic crisis. PMID:16363205

  15. Effects of homozygosity of the nude (rnu) gene in an inbred strain of rats: studies of lymphoid and non--lymphoid organs in different age groups of nude rats of LEW background at a stage in the gene transfer.

    PubMed

    Hougen, H P; Klausen, B

    1984-01-01

    Several age groups of nude homozygous rnu/rnu and heterozygous rnu/+ rats of the same genetic background at an early stage of back-crossing (LEW/Mol) were compared as to body and organ weights, histological appearance and cell density of lymphoid organs, haematological values and differential counts of bone marrow and peripheral blood. No thymic tissue was found in the nude animals. 7-week-old nudes were smaller than control animals and had relatively larger non-lymphoid organs and cell-depleted peripheral lymphoid organs. Other age groups showed little difference. Peripheral blood of nude rats showed no signs of lymphopaenia in contrast with the findings in nude mice. The number of thoracic duct lymphocytes was, however, significantly smaller in all age groups of the nude rats, and the bone marrow tended to contain fewer lymphocytes. PMID:10628778

  16. Can lenalidomide play a role in the management of scleritis?

    PubMed Central

    Al-Jafar, Hassan A; Abul, Nadia; Kumar, Niranjan; Al-Awadhi, Adel

    2013-01-01

    Lenalidomide is an immunomodulatory agent that was approved for the treatment of a monoclonal bone marrow disorders, myelodysplastic syndrome del(5q)(MDS del(5q)), in 2005; the drug was subsequently also approved for the treatment of refractory multiple myeloma, a bone marrow malignancy of the B-lymphocyte lineage. The purpose of this study is to report a case of MDS del(5q) in a female patient, which was most likely secondary to the immunosuppressive drugs that the patient was taking for scleritis. After lenalidomide treatment, the patient's haematological symptoms rapidly resolved and she became transfusion independent, with normal haemoglobin levels. This medication also helped control her dependence on high doses of oral prednisolone. The patient continued to receive treatment with low-dose lenalidomide, and her scleritis has been in long-term remission for 3?years. A larger prospective study can further define the role of lenalidomide in the management of scleritis. PMID:23780766

  17. Angiogenic factors in chronic lymphocytic leukaemia (CLL): Where do we stand?

    PubMed

    Aguirre Palma, Luis Mario; Gehrke, Iris; Kreuzer, Karl-Anton

    2015-03-01

    The role of angiogenesis in haematological malignancies such as chronic lymphocytic leukaemia (CLL) is difficult to envision, because leukaemia cells are not dependent on a network of blood vessels to support basic physiological requirements. Regardless, CLL cells secrete high levels of major angiogenic factors, such as vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and platelet derived growth factor (PDGF). Nonetheless, it remains unclear how most angiogenic factors regulate accumulation and delayed apoptosis of CLL cells. Angiogenic factors such as leptin, granulocyte colony-stimulating factor (G-CSF), follistatin, angiopoietin-1 (Ang1), angiogenin (ANG), midkine (MK), pleiotrophin (PTN), progranulin (PGRN), proliferin (PLF), placental growth factor (PIGF), and endothelial locus-1 (Del-1), represent novel therapeutic targets of future CLL research but have remained widely overlooked. This review aims to outline our current understanding of angiogenic growth factors and their relationship with CLL, a still uncured haematopoietic malignancy. PMID:25459668

  18. Immunotherapy of Malignant Disease Using Chimeric Antigen Receptor Engrafted T Cells

    PubMed Central

    Maher, John

    2012-01-01

    Chimeric antigen receptor- (CAR-) based immunotherapy has been under development for almost 25 years, over which period it has progressed from a new but cumbersome technology to an emerging therapeutic modality for malignant disease. The approach involves the genetic engineering of fusion receptors (CARs) that couple the HLA-independent binding of cell surface target molecules to the delivery of a tailored activating signal to host immune cells. Engineered CARs are delivered most commonly to peripheral blood T cells using a range of vector systems, most commonly integrating viral vectors. Preclinical refinement of this approach has proceeded over several years to the point that clinical testing is now being undertaken at several centres, using increasingly sophisticated and therapeutically successful genetic payloads. This paper considers several aspects of the pre-clinical and clinical development of CAR-based immunotherapy and how this technology is acquiring an increasing niche in the treatment of both solid and haematological malignancies. PMID:23304553

  19. Cutaneous neonatal lupus with cutis marmorata telangiectatica congenita-like lesions*

    PubMed Central

    Trevisan, Flávia; Cunha, Paulo Rowilson; Pinto, Clóvis Antonio Lopes; Cattete, Fernanda Gomes

    2013-01-01

    Neonatal lupus is a rare disease caused by the transplacental transfer of maternal autoantibodies to the foetus, characterized by transient clinical manifestations such as cutaneous, haematological, and hepatobiliary events or permanent such as congenital heart block. The typical cutaneous manifestations include erythematous, scaly, annular or arched lesions on the face, with slight central atrophy and photosensitivy, clinically and histologically similar to subacute cutaneous lupus. However, in some cases, the lesions may resemble those in cutis marmorata telangiectatica congenita, although this phenomenon is rare and only eight such cases have been reported to date. We report a case of cutaneous neonatal lupus with atypical lesions on the limbs, which had a reddish-purple marbled appearance, resembling the lesions in cutis marmorata telangiectatica congenita. PMID:23793203

  20. Demodicosis in nine prairie dogs (Cynomys ludovicianus).

    PubMed

    Jekl, Vladimir; Hauptman, Karel; Jeklova, Edita; Knotek, Zdenek

    2006-08-01

    This case report describes the clinical signs and treatment of demodicosis in nine pet prairie dogs (Cynomys ludovicianus). Clinical records including history, clinical examination, microscopic evaluation and fungal culture of the skin lesions were available for all nine animals. In seven animals, blood was obtained for haematological and biochemical analyses. Clinical signs comprised bilaterally symmetrical alopecia extending from the middle part of the back down to the skin of the thighs and the dorsal and ventral parts of the tail. Demodex mites were found in skin scrapings from all cases. Treatment with amitraz at 250 p.p.m. resulted in resolution of lesions and hair regrowth. This is the first report of demodicosis in this species. PMID:16827672