Note: This page contains sample records for the topic haematology from Science.gov.
While these samples are representative of the content of Science.gov,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of Science.gov
to obtain the most current and comprehensive results.
Last update: August 15, 2014.
1

Sports haematology.  

PubMed

While the crucial role of haemoglobin in aerobic exercise has been well accepted, there is still a great deal of controversy about the optimal haematological parameters in the athletic population. The initial part of this review will examine the question of anaemia in athletes. The most common finding in athletes is a dilutional pseudoanaemia that is caused by a plasma volume expansion, rather than an actual blood loss. It is not a pathological state and normalises with training cessation in 3 to 5 days. This entity should be distinguished from conditions associated with lowered blood counts, such as intravascular haemolysis or iron deficiency anaemia. The evaluation of true anaemia states in the athlete must take into account not only blood losses secondary to exercise, such as foot strike haemolysis or iron losses through sweat, but non-athletic causes as well. Depending on the age and sex of the athlete, consideration must be given to evaluation of the gastrointestinal or genitourinary systems for blood loss. Finally, a comprehensive nutritional history must be taken, as athletes, especially women, are frequently not consuming adequate dietary iron. The second section of the paper will deal with the very contentious issue of sickle cell trait. While there have been studies demonstrating an increased risk of sudden death in people with sickle cell trait, it is still quite rare and should not be used as a restriction to activity. Further, studies have demonstrated that patients with sickle cell trait have an exercise capacity that is probably normal or near normal. However, in the cases of sudden death, it has been secondary to rhabdomyolysis occurring among sickle cell trait athletes performing at intense exertion under hot conditions, soon after arriving at altitude. The recommendations are that athletes with sickle cell trait adhere to compliance with the general guidelines for fluid replacement and acclimatisation to hot conditions and altitude. The final section of the paper examines the issue of haematological manipulation for the purposes of ergogenic improvement. Although experiments with blood doping revealed improvements in running time to exhaustion and maximal oxygen uptake, the introduction of recombinant erythropoietin has rendered blood doping little more than a historical footnote. However, the improvements in performance are not without risk, and the use of exogenous erythropoietin has the potential for increased viscosity of the blood and thrombosis with potentially fatal results. Until a definitive test is developed for detection of exogenous erythropoietin, it will continue to be a part of elite athletics. PMID:10688281

Shaskey, D J; Green, G A

2000-01-01

2

Capillary blood collection in haematology  

PubMed Central

A capillary blood collection technique which facilitates the estimation of routine haematological parameters, including platelet count and sedimentation rate, is described. The technique requires 0·5 ml of blood, allows closer reproducibility than pipette collection methods, is suitable for monitoring blood counts in patients receiving cytotoxic agents, and can be integrated with semi-and fully-automated production lines. Images

Stuart, J.; Barrett, B. A.; Prangnell, D. R.

1974-01-01

3

Digital imaging of haematological morphology  

Microsoft Academic Search

Summary Microscopic images of haematological cells are now routinely photographed using digital cameras. Advances in technology mean that the quality of such digital images can now approach that viewed through a microscope. At the same time there is an emerging appreciation that such images can be used in many roles: digital images are now being used to construct digital virtual

C. V. HUTCHINSON; M. L. BRERETON; J. BURTHEM

2005-01-01

4

Improving haematology care in Wales.  

PubMed

Care for patients with a malignant haematological disorder in Wales has been revised significantly since the mid-1990s. Recommendations from expert groups stated that a uniformly high standard of care should be provided as close to the patient's home as possible and variations in practice that had been causing concern and distress to patients and their families be reduced. The All Wales Haematology Nurses Group was established to promote and disseminate standards and guidelines that would contribute to achieving these aims. The basis for the group's work was transformational leadership and collaborative practice. Work is ongoing and the group is planning to consolidate achievements to date through country-wide audit and professional education. PMID:11998241

Perett, Shelagh

2002-04-17

5

Primary immunoglobulin deficiency and haematological disorders.  

PubMed Central

Nine patients with immunoglobulin deficiency and various haematological disorders are presented. In all patients, recurrent infections had antedated the onset of the haematological disorder but, in most, the possibility of primary immunodeficiency had not been considered until after the haematological diagnosis had been established. The recognition of immunodeficiency is important since such patients may require steroids, immunosuppressive therapy or splenectomy. Gammaglobulin would appear to be the appropriate therapy in this situation. Infections were reduced in all 6 patients so treated.

French, M. A.; Dawkins, R. L.; Jackson, J. M.

1983-01-01

6

Seasonal variations of haematological parameters in athletes  

Microsoft Academic Search

The influence of training and competition workloads is crucial for evaluation of longitudinal haematological data in athletes.\\u000a There are only a few papers on the variation of haematological parameters during long-lasting periods and, especially, during\\u000a an entire competitive season. We summarized that some haematological parameters can be influenced by long-term training and\\u000a competition periods. Haemoglobin (Hb) and haematocrit (Ht) are

Giuseppe Banfi; Carsten Lundby; Paul Robach; Giuseppe Lippi

2011-01-01

7

Haematology of Persian Fallow Deer ( Dama mesopotamica )  

Microsoft Academic Search

:   The haematology of Persian fallow deer was studied and the means of various parameters were determined for sex and age groups.\\u000a For total samples, the mean ± standard deviation of haematological parameters were: red blood cells (RBC), 7.42 ± 1.27 × 1012\\/l; haematocrit (PCV), 38.83 ± 7.38%; haemoglobin (Hb), 148.0 ± 17.3 g\\/l; mean cell volume (MCV), 50.84 ±

M. Mohri; M. R. Aslani; N. Shahbazian

2001-01-01

8

Automated haematology analysis to diagnose malaria  

Microsoft Academic Search

For more than a decade, flow cytometry-based automated haematology analysers have been studied for malaria diagnosis. Although current haematology analysers are not specifically designed to detect malaria-related abnormalities, most studies have found sensitivities that comply with WHO malaria-diagnostic guidelines, i.e. ? 95% in samples with > 100 parasites\\/?l. Establishing a correct and early malaria diagnosis is a prerequisite for an

Germán Campuzano-Zuluaga; Thomas Hänscheid; Martin P Grobusch

2010-01-01

9

Clostridium difficile in haematological malignancy.  

PubMed Central

Twenty patients with haematological malignancies who developed Clostridium difficile bowel infection or colonisation are described. All isolates of C difficile were toxigenic in vitro and faecal cytotoxin (toxin B) was detected in 20/26 episodes. Ten of 20 episodes with detectable faecal cytotoxin were associated with typical antibiotic associated diarrhoea. In the other 10 episodes (nine patients), there was a severe unusual illness which was associated with detection of C difficile. The unusual features of the illness were pronounced jaundice (total bilirubin greater than or equal to 44 mumol/l), abdominal pain and distension, and initial constipation followed either by diarrhoea or by large bowel stasis. Four of these patients died within seven days. Bacteraemia was often a presenting feature in neutropenic patients subsequently shown to have C difficile. This was not the case in non-neutropenic patients. Bacteraemia was commonly polymicrobial and in two cases C difficile was isolated from blood culture. The clinical implications of recognition of this atypical C difficile associated syndrome are discussed.

Rampling, A; Warren, R E; Bevan, P C; Hoggarth, C E; Swirsky, D; Hayhoe, F G

1985-01-01

10

Interferon-alpha in childhood haematological malignancies  

Microsoft Academic Search

The application of cytostatics has brought about a breakthrough in the treatment of childhood haematological malignancies in the past 20 years. Chemotherapy appears to be least successful in the rare, low and very high mitotic index diseases, which often have an enormous tumour-burden. The suitability of chemotherapy in minimal residual leukaemia is also of some doubt. In these situations a

R. Simkó; K. Nagy

1996-01-01

11

Automated haematology analysis to diagnose malaria  

PubMed Central

For more than a decade, flow cytometry-based automated haematology analysers have been studied for malaria diagnosis. Although current haematology analysers are not specifically designed to detect malaria-related abnormalities, most studies have found sensitivities that comply with WHO malaria-diagnostic guidelines, i.e. ? 95% in samples with > 100 parasites/?l. Establishing a correct and early malaria diagnosis is a prerequisite for an adequate treatment and to minimizing adverse outcomes. Expert light microscopy remains the 'gold standard' for malaria diagnosis in most clinical settings. However, it requires an explicit request from clinicians and has variable accuracy. Malaria diagnosis with flow cytometry-based haematology analysers could become an important adjuvant diagnostic tool in the routine laboratory work-up of febrile patients in or returning from malaria-endemic regions. Haematology analysers so far studied for malaria diagnosis are the Cell-Dyn®, Coulter® GEN·S and LH 750, and the Sysmex XE-2100® analysers. For Cell-Dyn analysers, abnormal depolarization events mainly in the lobularity/granularity and other scatter-plots, and various reticulocyte abnormalities have shown overall sensitivities and specificities of 49% to 97% and 61% to 100%, respectively. For the Coulter analysers, a 'malaria factor' using the monocyte and lymphocyte size standard deviations obtained by impedance detection has shown overall sensitivities and specificities of 82% to 98% and 72% to 94%, respectively. For the XE-2100, abnormal patterns in the DIFF, WBC/BASO, and RET-EXT scatter-plots, and pseudoeosinophilia and other abnormal haematological variables have been described, and multivariate diagnostic models have been designed with overall sensitivities and specificities of 86% to 97% and 81% to 98%, respectively. The accuracy for malaria diagnosis may vary according to species, parasite load, immunity and clinical context where the method is applied. Future developments in new haematology analysers such as considerably simplified, robust and inexpensive devices for malaria detection fitted with an automatically generated alert could improve the detection capacity of these instruments and potentially expand their clinical utility in malaria diagnosis.

2010-01-01

12

Demodicosis of goats: Haematological and biochemical changes  

Microsoft Academic Search

iEDTA (2 mg ml- ) for haematology and sodium fluoride for glucose measurement (Hawk, 1965). Serum samples were assayed for total protein (Wooton, 1974), calcium (Clark and Collip, 1925) and inorganic phosphorus (Fiske and Subba Row, 1925). Hair samples from both control and mite-infested goats were collected in sterilized tubes and washed successively with de-ionized water, ethanol and ethyl ether

M. R. Dalapati; M. K. Bhowmik; S. Sarkar; N. K. Sasmal

1997-01-01

13

Immunomodulation in the treatment of haematological malignancies  

Microsoft Academic Search

Despite the continuous advances in immunology and cancer biology, haematological malignancies are often incurable. Conventional\\u000a chemotherapy and radiation are efficacious for some lymphoma and leukaemia, however relapse and progressive disease often\\u000a occurs. The evidence that the immune system can play an essential role in controlling cancer progression provide a basis for\\u000a the development of active therapies, such as immunization, aimed

Michela Cesco-Gaspere; Emma Morris; Hans J. Stauss

2009-01-01

14

Age-related changes in selected haematology parameters in rabbits  

Microsoft Academic Search

Even though there is an abundance of information on the reference values of haematological parameters in adult rabbits, a little is known about the changes in haematology in newborn rabbits or during their postnatal development. Therefore, the aim of our study was to investigate changes in red blood cells (RBC), white blood cells (WBC) and differential leukocyte counts in SPF

E. Jeklova; L. Leva; P. Knotigova; M. Faldyna

2009-01-01

15

[Zygomycosis (mucormycosis) in patients with haematologic malignancy].  

PubMed

Zygomycosis is an invasive and increasingly emerging life-threatening infection. Diabetes is the most common risk factor; however, zygomycosis has increased among patients with haematologic malignancy, which is now the second most common risk factor with an incidence of 16%. Rapid diagnosis and treatment are essential for patient survival. In the Danish literature, only a few cases have been reported. We present two cases of zygomycosis, both with a fatal outcome, and we discuss the pathogenesis and treatment of invasive zygomycosis. PMID:21276400

Jensen, Eva Magrethe Precht; Clemmensen, Stine; Bjørndal, Kristine; Krogdahl, Annelise

2011-01-31

16

Some haematological parameters in Malawian neonates.  

PubMed

Studies of haematological parameters were performed on 366 (177 male and 189 female) normal Malawian neonates with mean +/- s.d. birthweight of 2.99 +/- 0.37 (range 2.1-4.0) kg using a Nova Cell Track, Model Nova CT11. Cord anaemia (Cord Hb < 13.5g dl-1) was detected in 100 (27.3%) of the neonates. It was also shown that although the male babies had a significantly higher erythrocyte protoporphyrin level (p < 0.001) than the females, there were no significant differences (p > 0.05) in the red cell, white cell and platelet indices between the two sexes. When the haematological parameters of the 266 (72.7%) non-anaemic (Cord Hb > 13.5g dl-1) neonates were analysed, the mean +/- s.d. values which may serve as local reference standards were: Hb 16.0 +/- 1.7 (range 13.5-21.3) g dl-1, Hct 47.0 +/- 6.0 (range 36.5-67.5) percent, MCV 112.6 +/- 8.9 (range 72.2-131.0) fl, MCH 31.9 +/- 5.5 (range 24.4-48.5) pg, MCHC 33.5 +/- 2.8 (range 29.1-48.9) g dl-1 reticulocyte count 6.9 +/- 3.6 (range 1.2-25.0) percent, free erythrocyte protoporphyrin 3.3 +/- 0.9 (range 1.9-7.7) mgs ZPP gm-1 Hb, platelet count 269.9 +/- 57.7 (range 134.0-454.0) x 10(9) l-1 and total leucocyte count 12.3 +/- 4.8 (range 5.5-35.3) x 10(9) l-1. Further analysis of the differential wbc count disclosed normal levels of eosinophils and neutrophils similar to those given in standard haematology textbooks for Caucasian neonates; thus strengthening the belief that eosinophilia and relative neutropenia previously reported in adult Africans is not of genetic origin, but rather an acquired phenomena. PMID:7781547

Mukiibi, J M; Mtimavalye, L A; Broadhead, R; Mzula, E; Dzinyemba, W E; Merrick, R; Khoromana, C O; Ching'ani, G W

1995-01-01

17

Laboratory evaluation of the Sysmex SE9500 automated haematology analyser  

Microsoft Academic Search

Summary The Sysmex SE-9c500 is a new, fully automated haematology analyser, providing a complete blood count (CBC), including a five-part differential count (DC), with flagging of morphological abnormalities. The SE-9500 was evaluated according to guidelines published by the International Committee for Standardisation in Haematology (ICSH). The results demonstrated minimal carryover (< 0.01%) and excellent linearity for WBC, RBC, HGB and

Liming Peng; Xingjun Gao; Hong Jiang; Zhiyong Peng; Jun Su

2001-01-01

18

Tuberculosis in Patients with Haematological Malignancies  

PubMed Central

Tuberculosis (TB) is an infectious disease that causes more than 1 million deaths worldwide every year. In addition, it is estimated that one third of the world population is infected with M. tuberculosis in a latent state, which involves an eventual risk of progressing to active TB disease. Patients with immunodeficiencies, such as those suffering from haematological malignancies, have a greater risk of progressing to TB disease once infected. It is estimated that the Relative Risk of TB disease in patients with hematologic malignancies is 2–40 times that of the general population. The diagnosis of TB in these patients is often challenging as they often present clinical characteristics that are distinct to those of patients without any other underlying disease. Mortality due to TB is higher. Therefore, it is recommended to diagnose latent TB infection and consider preventive therapy that could avoid the progression from a latent state to active TB disease. There are currently two methods for diagnosing latent TB infection: the Tuberculin Skin Test (TST) and the Interferon-Gamma Release Assays (IGRA). Due to the lack of sensitivity in patients with immunodeficient conditions, a combined TST-IGRA testing is probably the best way for latent TB diagnosis in order to gain sensitivity. Treatment of latent TB infection and TB disease should follow the general principles to that in the general population.

Anibarro, Luis; Pena, Alberto

2014-01-01

19

Spleen in haematological malignancies: spectrum of imaging findings  

PubMed Central

Imaging morphology and metabolic activity of splenic lesions is of paramount importance in patients with haematological malignancies; it can alter tumour staging, treatment protocols and overall prognosis. CT, MRI and positron emission tomography (PET)/CT have been shown to be powerful tools for the non-invasive assessment of splenic involvement in various haematological malignancies. Since many haematological malignancies and non-neoplastic conditions can involve the spleen and imaging manifestations can overlap, imaging and clinical findings outside of the spleen should be looked for to narrow the differential diagnosis; confirmation can be obtained by pathological findings. Radiologists should be familiar with the cross-sectional imaging patterns of haematological malignancies involving the spleen as well as non-neoplastic splenic findings common in these patients to facilitate their care and follow-up. This pictorial review provides the common and uncommon imaging appearances and complications of various haematological malignancies involving the spleen on CT, MRI and PET/CT, and common pitfalls in diagnosis.

Saboo, S S; Krajewski, K M; O'Regan, K N; Giardino, A; Brown, J R; Ramaiya, N; Jagannathan, J P

2012-01-01

20

Haematology of clinically normal and sick captive reindeer (Rangifer tarandus).  

PubMed

A retrospective analysis of haematological values from clinically normal captive reindeer (Rangifer tarandus) showed that the red cell count, haemoglobin level, packed cell volume and lymphocyte count were higher and the erythrocyte sedimentation rate and eosinophil count were lower in juveniles than in adults. Newborn animals were anaemic compared with juveniles and adults and had high reticulocyte counts. The values from healthy reindeer were used to identify abnormal haematological variations in a number of sick animals. It was shown that reindeer exhibit similar haemopathological responses to those of other artiodactyla, with increases in the erythrocyte sedimentation rate and fibrinogen level being of particular diagnostic significance. Eosinophilia was the only abnormal haematological finding in individuals with subclinical infections of intestinal parasites. PMID:2321339

Catley, A; Kock, R A; Hart, M G; Hawkey, C M

1990-03-10

21

Age-related changes in selected haematology parameters in rabbits.  

PubMed

Even though there is an abundance of information on the reference values of haematological parameters in adult rabbits, a little is known about the changes in haematology in newborn rabbits or during their postnatal development. Therefore, the aim of our study was to investigate changes in red blood cells (RBC), white blood cells (WBC) and differential leukocyte counts in SPF New Zealand White rabbits from the age of one day to 20 weeks. Significant age-related changes during the first four weeks of life were detected. These included an increase of RBC and WBC, reversal of the neutrophil/lymphocyte ratio and increase of total counts of eosinophils and basophils. From the age of six weeks of life, all of the studied haematological parameters were comparable to those of adult rabbits. PMID:19041105

Jeklova, E; Leva, L; Knotigova, P; Faldyna, M

2009-06-01

22

Normal and clinical haematology of captive cranes (gruiformes)  

Microsoft Academic Search

Fall blood counts on 56 clinically normal cranes of nine different species have provided reference values for the interpretation of haematological changes in 13 cranes presenting with abnormal clinical signs. Hypochromic anaemia, heterophilia and lymphocytosis were found in birds with probable Mycobacterium avium infection and heterophilia and lymphocytosis in birds with bumblefoot, arthritis, nephrosis and cardiac myo?pathy. In several of

Christine Hawkey; J. H. Samour; D. G. Ashton; M. G. Hart; R. N. Cindery; J. M. Ffinch; D. M. Jones

1983-01-01

23

Is intensive care justified for patients with haematological malignancies?  

Microsoft Academic Search

The course of 260 adults with haematological malignancies admitted to a medical intensive care unit was studied to evaluate the value of life support techniques and to research predictive factors. The overall in the medical intensive care unit (MICU) and hospital mortality rates were respectively 43% (113 patients) and 57% (148 patients). Among survivors, 64% (49 patients) were still alive

F. Brunet; J. J. Lanore; J. F. Dhainaut; F. Dreyfus; J. F. Vaxelaire; S. Nouira; T. Giraud; A. Armaganidis; J. F. Monsallier

1990-01-01

24

Haematological and clinicochemical blood profiles in slaughter pigs  

Microsoft Academic Search

In a cohort study, 40 pig finishing herds were selected twenty pig herds with a low and twenty pig herds with a high prevalence of several pathological lesions recorded at slaughter in a six?month period before the start of the study. Blood samples were taken from 20 pigs per herd at the end of the finishing period to investigate haematological

A. R. W. Elbers; G. H. M. Counotte; M. J. M. Tielen

1992-01-01

25

Advances in Haematological Pharmacotherapy in 21st Century  

PubMed Central

Last quarter of twentieth century and the first 10 years of 21st century has seen phenomenal development in haematological pharmacotherapy. Tailor made chemotherapeutic agents, vast array of monoclonal antibodies, epigenetic modifiers, growth factors for red cells white cells and platelets, peptidomimetics as growth factors, newer thrombin inhibitors, safer plasma derived protein molecules, recombinant molecules, newer immunomodulators, enzyme replacement therapy and above all a plethora of targetted molecules targeting innumerable pathways involved in cell division, growth, proliferation and apoptosis has given immense number of clinically usable molecules in the hand of modern haematologists to treat diverse hitherto untreatable haematological disorders effectively. In addition many old molecules are finding newer uses in diverse fields, thalidomide as an antiangiogenic molecule is a prime example of this genre. Present overview has tried to capture this rapidly evolving area in a broad canvas without going into details of indications and contraindications of the use of various drugs.

Ghosh, Kinjalka

2010-01-01

26

Molecular cytogenetics in haematological malignancy: current technology and future prospects  

Microsoft Academic Search

Cytogenetics has played a pivotal role in haematological malignancy, both as an aid to diagnosis and in identifying recurrent\\u000a chromosomal rearrangements, an essential prerequisite to identifying genes involved in leukaemia and lymphoma pathogenesis.\\u000a In the late 1980s, a series of technologies based around fluorescence in situ hybridisation (FISH) revolutionised the field.\\u000a Interphase FISH, multiplex-FISH (M-FISH, SKY) and comparative genomic hybridisation

Lyndal Kearney; Sharon W. Horsley

2005-01-01

27

Rheumatologic manifestations of benign and malignant haematological disorders  

Microsoft Academic Search

Diseases of blood and lymphoreticular system can have multisystem manifestations. Rheumatologic involvement has been reported\\u000a in association with many benign and malignant haematological disorders; these patients are equally likely to present to both\\u000a clinical rheumatologists and haematologists. This review focuses on the well-described rheumatologic features, other occasionally\\u000a reported rheumatologic manifestations and unusual musculoskeletal complications related to the treatment in patients

Vinod Ravindran; Parameswaran Anoop

28

Haematological changes in experimental trypanosomiasis in Barbari goats  

Microsoft Academic Search

Haematological changes due to Trypanosoma evansi infection were studied in 12 Barbari male goats of 6–9 months of age. These were divided in two groups, A and B, consisting of eight infected and four control animals, respectively. The animals were kept in strict hygienic conditions and on a zero grazing schedule. Animals of group A were exposed to 1×106T. evansi

D. K. Sharma; Chauhan; V. K. Saxena; R. D. Agrawal

2000-01-01

29

MicroRNAs and haematology: small molecules, big function.  

PubMed

MicroRNAs are a recently discovered class of small ( approximately 22nt) endogenously expressed translational-repressor RNAs that play key roles in many cellular pathways and whose aberrant expression appears to be a common feature of malignancy. MicroRNAs are expressed in specific haematological cell types and play important regulatory roles in early haematopoietic differentiation, erythropoiesis, granulocytosis, megakaryocytosis and lymphoid development. Additionally, there is an emerging body of research to suggest that microRNAs play an important role in the pathology of haematological malignancies. MicroRNAs have been found to act as both tumour suppressor molecules [e.g. MIRN15A (miR-15a), MIRN16-1 (miR-16-1)] in leukaemias and have oncogenic properties [e.g. MIRN155 (miR-155) and MIRN17-92 (miR-17-miR-92) cluster] in lymphomas. This review discusses the rapidly accumulating research that points to the major role microRNAs play in both haematopoiesis and haematological malignancy. PMID:17539773

Lawrie, Charles H

2007-06-01

30

Haematological findings in children with inborn errors of metabolism.  

PubMed

Early detection and therapy of haematological abnormalities and/or diseases may improve the prognosis of metabolic disorders. Accordingly, we aimed to evaluate the frequency and types of haematological abnormalities in children[-31pc] with various inherited metabolic disorders. The study group comprised 46 children with metabolic disorders who were followed at the Pediatric Metabolism Unit and were referred to the Pediatric Hematology Unit for evaluation of anaemia between June 2000 and 2005. The mean age of the children was 55.2 +/- 64.8 months at haematological evaluation (range 1 month-18 years, median 22.0 months); 16 were female and 30 were male. Of these 46 patients with anaemia, 25 of (54.3%) had anaemia of chronic disease (ACD), 9 (19.6%) had iron-deficiency anaemia (IDA), 7 (15.2%) had megaloblastic anaemia due to vitamin B(12) deficiency, 3 (6.5%) had chronic haemolytic anaemia, 2 (4.3%) had autoimmune haemolytic anaemia, 1 had beta-thalassaemia major, and 1 had hereditary spherocytosis. In addition to the anaemia, bicytopenia or pancytopenia was found in 8 of 46 children (17.4%). The study indicated that in organic acidaemias including methylmalonic acidaemia, propionic acidaemia, isovaleric acidaemia, and argininosuccinic acidaemia, the majority of patients had ACD (75%), which was followed by vitamin B(12) deficiency anaemia and IDA (p < 0.001). In PKU, both nutritional anaemias and ACD were present at about same frequency: 46.7% and 40%, respectively (p > 0.05). This study suggested that congenital anaemias such as hereditary spherocytosis or thalassaemias should be kept in mind as a coexisting haematological diseases in young patients with inborn errors of metabolism. In conclusion, ACD and nutritional anaemias are the most prevalent anaemias seen in patients with inborn errors of metabolism. Early detection of the disease, early administration of specific diet, and close monitoring of the patients are very important factors to prevent the development of haematological diseases in patients with inborn errors of metabolism. PMID:16906472

Tavil, Betul; Sivri, Hatice Serapl Kalkanoglu; Coskun, Turgay; Gurgey, Aytemiz; Ozyurek, Emel; Dursun, Ali; Tokatli, Aysegul; Altay, Cigdem; Gumruk, Fatma

2006-10-01

31

Haematological response of fish Channa punctatus (Bloch) to chronic zinc exposure.  

PubMed

Sub-lethal concentrations of zinc administered to freshwater teleost, Channa punctatus for a period of 135 days brought about significant haematological alterations. The RBC count, haemoglobin and haematocrit content progressively decreased while WBC count, MCV, MCH and MCHC increased. Alterations in the haematological parameters were dose and duration dependent and can thus serve as a useful physiological index. PMID:16334279

Tyagi, Anupama; Srivastava, Neera

2005-06-01

32

Haematological and clinical biochemistry findings in captive juvenile guanacos ( Lama guanicoe Müller 1776) in central Chile  

Microsoft Academic Search

The purpose of this study was to describe haematological and blood biochemistry findings of farmed guanacos in central Chile, in order to establish reference values for this species in captivity. Haematological and clinical biochemical measurements were performed on blood and plasma respectively, from 40 clinically healthy guanacos (20 females and 20 castrated males), aged between 2 and 3 years. The

B. Zapata; V. Fuentes; C. Bonacic; B. González; G. Villouta; F. Bas

2003-01-01

33

Haematological profiles in pure bred cattle naturally infected with Theileria annulata in Saudi Arabia  

Microsoft Academic Search

The Abbott Cell Dyn 3500 haematology analyzer was employed to study haematological parameters in 41 adult and young Friesian cattle naturally infected with Theileria annulata in the Qassim region of Saudi Arabia. Comparison was made with clinically healthy adult and young Friesian cattle. Changes in blood parameters in T. annulata-infected cattle indicated severe macrocytic hypochromic anaemia, panleukopenia, lymphocytopenia, eosinopenia, neutropenia

O. H Omer; K. H El-Malik; O. M Mahmoud; E. M Haroun; A Hawas; D Sweeney; M Magzoub

2002-01-01

34

Atlas of Genetics and Cytogenetics in Oncology and Haematology  

NSDL National Science Digital Library

The Atlas of Genetics and Cytogenetics in Oncology and Haematology, a peer-reviewed online journal and database provided by Infobiogen, is "devoted to genes, cytogenetics, and clinical entities in cancer, and cancer-prone diseases." Users can search the materials by genes, leukaemias, solid tumors, cancer prone diseases, and chromosomes. Researchers can find links to scientific societies and meetings. Students can explore educational materials on Mendelian and non-Mendelian Inheritance, chromosomes, population genetics, and additional human genetics topics. The website also offers reviews and case reports.

35

Haematological and biochemical studies in tigers ( Panthera tigris tigris )  

Microsoft Academic Search

Haematological and biochemical studies were conducted on 12 clinically healthy tigers of Central India. The range and mean\\u000a (with one standard deviation), respectively for the parameters examined were: red blood cells, 4.66 to 9.15, 7.9?±?1.42, 106\\/?l; haemoglobin, 7.8 to 13.8, 12.8?±?1.65 g\\/dl; packed cell volume, 36 to 45, 38?±?2.54; icterus index, 2 to 5, 2?±?1.51 U;\\u000a erythrocyte sedimentation rate, 14 to 26,

A. B. Shrivatav; K. P. Singh; S. K. Mittal; P. K. Malik

36

Long Non-Coding RNAs in Haematological Malignancies  

PubMed Central

Long non-coding RNAs (lncRNAs) are functional RNAs longer than 200 nucleotides in length. LncRNAs are as diverse as mRNAs and they normally share the same biosynthetic machinery based on RNA polymerase II, splicing and polyadenylation. However, lncRNAs have low coding potential. Compared to mRNAs, lncRNAs are preferentially nuclear, more tissue specific and expressed at lower levels. Most of the lncRNAs described to date modulate the expression of specific genes by guiding chromatin remodelling factors; inducing chromosomal loopings; affecting transcription, splicing, translation or mRNA stability; or serving as scaffolds for the organization of cellular structures. They can function in cis, cotranscriptionally, or in trans, acting as decoys, scaffolds or guides. These functions seem essential to allow cell differentiation and growth. In fact, many lncRNAs have been shown to exert oncogenic or tumor suppressor properties in several cancers including haematological malignancies. In this review, we summarize what is known about lncRNAs, the mechanisms for their regulation in cancer and their role in leukemogenesis, lymphomagenesis and hematopoiesis. Furthermore, we discuss the potential of lncRNAs in diagnosis, prognosis and therapy in cancer, with special attention to haematological malignancies.

Garitano-Trojaola, Andoni; Agirre, Xabier; Prosper, Felipe; Fortes, Puri

2013-01-01

37

Can haematological tests predict cardiovascular risk? The 2005 Kettle Lecture.  

PubMed

The risk of venous or arterial thrombosis is routinely assessed by clinical variables (risk factors) supplemented by measurement of blood lipids and glucose for arterial thrombotic events. Haematological tests that might play a role in risk prediction include haemostatic variables, haematocrit and inflammatory markers (erythrocyte sedimentation rate, plasma viscosity, white cell count). Recent epidemiological studies of these phenotypes and related genotypes are reviewed. For the risk prediction of first venous thrombosis, screening for thrombophilias in 'high-risk' situations does not appear clinically effective or cost-effective; with the possible exception of women considering oral hormone replacement therapy. General screening after a first venous event to predict recurrence (or risk in asymptomatic relatives) does not appear effective; with the possible exception of d-dimer, which requires further study. For risk prediction of first arterial thrombosis, screening adds little to prediction by current clinical risk scores. Screening of persons after a first arterial event, or with atrial fibrillation (e.g. with D-dimer for stroke prediction), requires further study. In conclusion, haematological tests have very limited roles in the prediction of cardiovascular risk, and should only be used according to evidence-based guidelines. The need for management studies is highlighted. PMID:16643425

Lowe, Gordon D O

2006-05-01

38

Haematology in the Republic of Macedonia: present situation and brief history.  

PubMed

(Full text is available at http://www.manu.edu.mk/prilozi). The development of clinical haematology in Macedonia has taken place over the past nine decades. The greatest expansion of its development took place in the second half of the 20th century. The oficial start of clinical haematology dates from 1956, when the Department of Haematology was founded within the fra-mework of the Internal Medicine Clinic in Skopje. In the beginning, haematology represented a form of virtual sub-specialty, but its expansion was so progressive and rapid that it reached the highest peaks of Yugoslav haematology in those times. The period from 1968 to 1979 was a period of integral development of haema-tology and blood-transfusion science in Macedonia. Nowadays, the autonomous Public Health Institution, the University Hematology Clinic, is a unique healthcare, educational and scientific establishment in the Republic of Macedonia in its field of work. The diagnostics algorithm comprises cyto-morphologic and cyto-chemical analysis, through immunologic characterization with the assistance of a flow cytometer, to sophisticated molecular analysis for detecting genetic abnormalities. The therapeutic approach is based upon modern poly-haemotherapeutic protocols, application of monoclonal antibodies, immuno-modulatory agents, molecular target therapy and the use of alogeneic and autologous transplantation of fresh bone-marrow and frozen haemopoietic stem-cells. The current motto of the Haematology Clinic is: always help those who seek help, provide precise and early diagnostics, and apply all up-to-date therapeutic strategies, scientific research, continual education and day-to-day implementation of the latest achievements in the field of haematology in daily practice. Key words: haematology, history, chemotherapy, flow-cytometry, molecular analysis, stem cell transplant, target therapy, immuno-modulatory agents. PMID:23921481

Panovska-Stavridis, I; Cevreska, L

2013-01-01

39

Targeting Metabolism and Autophagy in the Context of Haematologic Malignancies  

PubMed Central

Autophagy is a cellular process that maintains the homeostasis of the normal cell. It not only allows for cell survival in times of metabolic stress with nutrient recycling but also is able to lead to cell death when required. During malignant transformation the cell is able to proliferate and survive. This is due to altered cell metabolism and the presence of altered genetic changes that maintain the cell survival. Metabolism was considered an innocent bystander that was a consequence of the increased nutrient requirement for the survival and proliferation of haematological malignancies. The interdependency of metabolism and cellular mechanisms such as autophagy are becoming more evident and important. This interdependence contributes to increased cancer progression and drug resistance. In this paper we aim to discuss autophagy, how it pertains to metabolism in the context of hematologic malignancies, and the implications for therapy.

Banerji, Versha; Gibson, Spencer B.

2012-01-01

40

Haematological and haemorheological changes associated with cigarette smoking.  

PubMed Central

A prospective study was undertaken to establish the haematological and haemorheological changes associated with long standing cigarette smoking in 20 heavy smokers and to assess whether any such changes were reversible after smoking was stopped. Highly significant differences were observed in whole blood viscosity, plasma viscosity, plasma fibrinogen concentrations, packed cell volume, and carboxyhaemoglobin concentrations between smokers and non-smokers. Ten of the subjects were followed up for two weeks after stopping smoking by which time whole blood viscosity and carboxyhaemoglobin concentrations had improved significantly and indications of improvement could be seen in all other measurements. Two of these subjects were further followed up for two months when all the measured variables were comparable with those in non-smokers. No correlation could be established between carboxyhaemoglobin concentration and any of the other variables. Although patients' compliance may be difficult to obtain, further prospective studies would be required to confirm our findings.

Galea, G; Davidson, R J

1985-01-01

41

Haematological reference values for east African wild ungulates.  

PubMed

Basic haematological values were obtained in the field from shot, clinically normal, wild East African ungulates. The species studied were: Thomson's gazelle (Gazella thomsonii), Grant's gazelle (Gazella grantii), blue wildebeest (Conochaetes taurinus), Coke's hartebeest (Alcelaphus buselaphus cokii), impala (Aepyceros melampus) and Burchell's zebra (Equus burchelli). Red cell parameters compared well with previous reports although the mean cell haemoglobin concentrations were higher. The white cell counts were considerably lower in all species. The red and white cells of all species studied had standard mammalian morphology. Generally, the field equipment used worked well under the conditions encountered, although the field microscope made cell counting troublesome and the heat made the preservation of fixed slides difficult. PMID:8460271

Sleeman, J M; Widdowson, M A

1993-03-01

42

Haematological and blood chemical values from Bothrops ammodytoides (ophidia-crotalidae) in captivity  

Microsoft Academic Search

In order to establish reference haematological and blood chemistry parameters, blood samples were obtained from 50 healthy\\u000a specimens ofBothrops ammodytoides kept in captivity. The haematological parameters determined were: red blood cell count (RBC); total leucocyte (WBC) and differential\\u000a leucocyte cell count; thrombocyte count; haematocrit (PCV); haemoglobin concentration; mean corpuscular volume (MCV); mean\\u000a corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration

J. C. Troiano; J. C. Vidal; E. F. Gould; G. Malinskas; J. Gould; M. Scaglione; L. Scaglione; J. J. Heker; C. Simoncini; H. Dinápoli

1999-01-01

43

CLINICAL AND HAEMATOLOGICAL STUDIES IN DOGS, EXPERIMENTALLY INFECTED WITH TRICHURIS VULPIS  

Microsoft Academic Search

Summary Kirkova, Z., P. Petkov & D. Goundasheva, 2005. Clinical and haematological studies in dogs, experimentally infected with Trichuris vulpis. Bulg. J. Vet. Med., 8, No 2, 141?148. The experiment was performed on 9 mixed-breed dogs (6 infected and 3 non-infected controls). The experimental infection was provoked with Trichuris vulpis (10000 eggs\\/kg). The clinical signs and the following haematological parameters

Z. KIRKOVA; P. PETKOV; D. GOUNDASHEVA

2005-01-01

44

Statin Use Is Associated with Reduced Risk of Haematological Malignancies: Evidence from a Meta-Analysis  

PubMed Central

Background Several observational studies have shown that statin use may modify the risk of haematological malignancies. To quantify the association between statin use and risk for haematological malignancies, we performed a detailed meta-analysis of published studies regarding this subject. Methods We conducted a systematic search of multiple databases including PubMed, Embase, and Cochrane Library Central database up to July 2013. Fixed-effect and random-effect models were used to estimate summary relative risks (RR) and the corresponding 95% confidence intervals (CIs). Potential sources of heterogeneity were detected by meta-regression. Subgroup analyses and sensitivity analysis were also performed. Results A total of 20 eligible studies (ten case-control studies, four cohort studies, and six RCTs) reporting 1,139,584 subjects and 15,297 haematological malignancies cases were included. Meta-analysis showed that statin use was associated with a statistically significant 19% reduction in haematological malignancies incidence (RR?=?0.81, 95% CI [0.70, 0.92]). During subgroup analyses, statin use was associated with a significantly reduced risk of haematological malignancies among observational studies (RR?=?0.79, 95% CI [0.67, 0.93]), but not among RCTs (RR?=?0.92, 95% CI [0.77, 1.09]). Conclusions Based on this comprehensive meta-analysis, statin use may have chemopreventive effects against haematological malignancies. More studies, especially definitive, randomized chemoprevention trials are needed to confirm this association.

Yi, Xiao; Jia, Wei; Jin, Yin; Zhen, Shang

2014-01-01

45

Clinical haematology of the great bustard (Otis tarda).  

PubMed

The haematological parameters of healthy great bustards (Otis tarda L.) have been determined. The values obtained were red cell count (3.0 x 10(12) +/- 0.2 x 10(12/)1), white cell count (33.0 x 10(9) +/- 2.6 x 10(9)/1), haematocrit value (0.51 +/- 0.01 1/1), haemoglobin (13.0 +/- 0.3 g/dl), mean corpuscular volume (178.7 +/- 12.5 fl), mean cell haemoglobin concentration (25.0 +/- 0.6 g/dl), mean corpuscular haemoglobin (42.5 +/- 3.2 pg), differential white cell count: heterophils (22.5 x 10(9) +/- 0.7 x 10(9)/1), lymphocytes (6.0 x 10(9)+/-0.7 x 10(9)/1), eosinophils (2.7 x 10(9) +/- 0.3 x 10(9)/1) and monocytes (1.8 x 10(9)+/-0.2 x 10(9)/1). PMID:18680064

Jimenez, A; Barrera, R; Sanchez, J; Cuenca, R; Rodriguez, J; Andres, S; Mane, M C

1991-12-01

46

Endotoxin-triggered haematological interactions in Fusobacterium necrophorum infections.  

PubMed

The haematological mechanisms in the course of liver abscess formation were evaluated. They were examined by employing viable cells of Fusobacterium necrophorum subsp. necrophorum and Fusobacterium necrophorum subsp. funduliforme in comparison with their endotoxins. Whole cell infection with F.n. necrophorum led to neutrophilia and to a concomitant monocytosis in parallel with those responses induced by the in vivo injection of its endotoxin. Viable infection with F.n. funduliforme was characterized by a sustained endotoxin-related monocytosis against neutropenia. The stimulatory impact of endotoxin on monocytes when released from a viable F.n. funduliforme infection suggested an inherently peculiar mechanism which differed from the induction of both neutrophilia and monocytosis when F.n. funduliforme endotoxin was administered alone. The neutrophilic inducing capacity of the F.n. necrophorum endotoxin was equally illustrated by its positive chemotactic effect on polymorphonuclear neutrophils in vitro. The data presented here emphasize the virulence of F.n. necrophorum viewed in reference to changes in leucocyte trafficking and as complemented by a relatively high endotoxin content. PMID:10817519

Garcia, G G; Goto, Y; Shinjo, T

2000-01-01

47

Guidelines on Vaccinations in Paediatric Haematology and Oncology Patients  

PubMed Central

Objective. Vaccinations are the most important tool to prevent infectious diseases. Chemotherapy-induced immune depression may impact the efficacy of vaccinations in children. Patients and Methods. A panel of experts of the supportive care working group of the Italian Association Paediatric Haematology Oncology (AIEOP) addressed this issue by guidelines on vaccinations in paediatric cancer patients. The literature published between 1980 and 2013 was reviewed. Results and Conclusion. During intensive chemotherapy, vaccination turned out to be effective for hepatitis A and B, whilst vaccinations with toxoid, protein subunits, or bacterial antigens should be postponed to the less intensive phases, to achieve an adequate immune response. Apart from varicella, the administration of live-attenuated-virus vaccines is not recommended during this phase. Family members should remain on recommended vaccination schedules, including toxoid, inactivated vaccine (also poliomyelitis), and live-attenuated vaccines (varicella, measles, mumps, and rubella). By the time of completion of chemotherapy, insufficient serum antibody levels for vaccine-preventable diseases have been reported, while immunological memory appears to be preserved. Once immunological recovery is completed, usually after 6 months, response to booster or vaccination is generally good and allows patients to be protected and also to contribute to herd immunity.

Cesaro, Simone; Giacchino, Mareva; Fioredda, Francesca; Barone, Angelica; Battisti, Laura; Bezzio, Stefania; Frenos, Stefano; De Santis, Raffaella; Livadiotti, Susanna; Marinello, Serena; Zanazzo, Andrea Giulio; Caselli, Desiree

2014-01-01

48

Characteristics of invasive aspergillosis in neutropenic haematology patients (Sousse, Tunisia).  

PubMed

Although scarce, available data suggest that the epidemiology of invasive aspergillosis (IA) in North Africa differs from northern countries, where more than 80 % is caused by Aspergillus fumigatus. This study aimed at describing the epidemiology of IA in the region of Sousse, Tunisia, and at assessing the usefulness of the available diagnostic tools. For 2 years, clinical and mycological data were prospectively collected from 175 neutropenia episodes of 91 patients hospitalised in the haematology department at the Farhat Hached hospital in Sousse (Tunisia). Screening for galactomannan antigen was positive in 40 % of neutropenia episodes; Aspergillus PCR was positive in 42 % of the tested sera. Nine patients were classified as probable and two as possible IA according to the EORTC/MSG criteria. Twelve patients who prematurely died, had no CT scan and could not be classified. Fifty-six Aspergillus spp. were isolated in 53 (6.5 %) sputa collected from 35 (20 %) patients. The following species were identified with MALDI-TOF mass spectrometry and DNA sequencing: A. niger, 35 %; A. flavus, 38 %; A. tubingensis, 19 %; A. fumigatus, 4 %; A. westerdijkiae, 2 % and A. ochraceus, 2 %. Our findings highlight the epidemiological features of IA in Tunisia, which is characterised by the predominance of Aspergillus spp. from sections Nigri and Flavi. PMID:24728707

Gheith, Soukeina; Saghrouni, Fatma; Bannour, Wadiaa; Ben Youssef, Yosra; Khelif, Abderrahim; Normand, Anne-Cécile; Ben Said, Moncef; Piarroux, Renaud; Njah, Mansour; Ranque, Stéphane

2014-06-01

49

Haematology of foals up to one year old.  

PubMed

Packed cell volume, haemoglobin concentration, erythrocyte counts, erythrocyte indices, serum iron, iron binding capacities, total and differential leucocyte counts, platelet counts, total plasma protein, fibrinogen, haptoglobin and icterus index values were determined at 14 different ages in eight Thoroughbred and 14 Quarterhorse foals during the first year of life. Absolute neutrophil numbers in blood decreased and lymphocyte numbers increased during the first months. Absolute eosinophil numbers tended to increase until three months old. Haemoglobin concentration and packed cell volume decreased significantly during the first two weeks and generally stayed in the lower portion of adult horse normal ranges during the remainder of the first year. Mean cell volume decreased to minimum values at approximately four months and then gradually increased. Serum iron was high at birth and decreased rapidly to a minimum at three days old. Total iron-binding capacity increased to a maximum at one month. Serum haptoglobin was generally within the adult normal range at birth. A moderate, but significant, decrease occurred at one week old. Fibrinogen concentration increased to a maximum at five months. Icterus index values decreased rapidly during the first two weeks of life. Minor changes occurred in other parameters measured. Possible causes for the various changes in haematological parameters are discussed. PMID:6479131

Harvey, J W; Asquith, R L; McNulty, P K; Kivipelto, J; Bauer, J E

1984-07-01

50

Incidence and pattern of liver involvement in haematological malignancies.  

PubMed

The incidence and pattern of liver involvement in 127 liver specimens (2 biopsy and 125 autopsy specimens) from cases of acute myelogenous leukaemia (25), chronic myelogenous leukaemia (7), acute lymphatic leukaemia (5), chronic lymphatic leukaemia (9), multiple myeloma (25), low-grade non-Hodgkin's lymphoma (25), high-grade non-Hodgkin's lymphoma (24) and myeloproliferative diseases (7) were investigated histologically and immunohistochemically. Liver infiltration was found frequently in chronic leukaemia and myeloproliferative diseases (80-100%), acute leukaemia (60-70%) and non-Hodgkin's lymphoma (50-60%), but was significantly less common in multiple myeloma (32%) than in any of the other diagnostic groups. Hepatomegaly was found in over 50% of cases in all the diagnostic groups, but was not always associated with infiltration. Diffuse, non-destructive infiltration was most common: in acute myelogenous leukaemia, both the portal triads and sinusoids were usually involved; in chronic myelogenous leukaemia, multiple myeloma and myeloproliferative diseases, infiltration was mainly sinusoidal; and in lymphatic leukaemia and non-Hodgkin's lymphoma the portal triads were mainly involved. Nodular infiltration was seen in multiple myeloma and non-Hodgkin's lymphoma. The primary tumours and liver infiltrates generally exhibited the same immunophenotype, although reactivity with the antibody L26 (CD20) was only found in the primary lesion in many high-grade B-cell lymphomas. Thus, liver involvement is common in haematological malignancies, but the incidence and pattern of infiltration vary amongst the different types. PMID:9842637

Walz-Mattmüller, R; Horny, H P; Ruck, P; Kaiserling, E

1998-01-01

51

Diurnal variations in serum biochemical and haematological measurements.  

PubMed

Twenty five biochemical and haematological measurements were determined on nonfasting blood and serum samples collected between 9 am and 7 pm from a representative group of 7685 British middle-aged men. Most measurements showed significant diurnal variations, but only for bilirubin, phosphate, and triglyceride did time of day account for more than 5% of the between subject variance. Serum bilirubin concentrations showed a pronounced downward trend in the afternoon, the mean value after 6 pm being 30% lower than the mean value in the morning. Mean serum triglyceride and phosphate concentrations increased steadily through the day. Mean concentrations of potassium, haemoglobin, and haematocrit and red cell count were higher in the morning, while urea and creatinine concentrations and white cell count had higher means in the afternoon. Glucose showed a pattern consistent with short term response to meals. The effects of these diurnal trends on routine use of biochemical tests needs careful consideration, and a greater understanding of their biological mechanisms is required. PMID:2921359

Pocock, S J; Ashby, D; Shaper, A G; Walker, M; Broughton, P M

1989-02-01

52

Clinical, haematological and biochemical responses of sheep undergoing autologous blood transfusion  

PubMed Central

Background This study aimed to evaluate the clinical, haematological and biochemical responses to autologous blood transfusion and the feasibility of this practice in sheep. Thus, we used eight male, 8?months old sheep, weighing on average 30?kg, from which 15?mL/kg of whole blood was collected and stored in CPDA-1 bags. Blood samples were refrigerated for 8?days and subsequently re-infused. The clinical, haematological and biochemical parameters were evaluated before blood collection and reinfusion, after 10 minutes of collection and reinfusion, after 3, 6, 12, 24, 48, 96 and 192 hours after collection and reinfusion. Results With respect to clinical parameters, we observed a decrease in heart rate after 24, 48 and 196 hours from reinfusion compared to basal values (p?Haematological variables including globular volume and erythrocyte counts showed a significant decrease (p?haematological responses of sheep, indicating that the technique proposed is safe and can be applied in the clinical practice of this species. The 8 d period was not sufficient for complete recovery of the haematological parameters after blood collection.

2012-01-01

53

Biochemical and haematological profile of pheasant hens during the laying period.  

PubMed

The present paper provides new experimental data on the biochemical and haematological profile of blood in pheasant hens, and points out the changes in both biochemical and haematological parameters that occur during the laying period. Significant effects of egg laying on both the biochemical and the haematological blood parameters of pheasant hens were found. Biochemical analyses revealed a significant increase in the metabolites cholesterol, uric acid, lactate, the enzyme aspartate aminotransferase (AST) and the minerals calcium and phosphorous, as well as a significant decrease in total protein, albumin and glucose in the course of the laying period. Haematological analyses revealed a significant increase in the count of leukocytes, lymphocytes, eosinophils, basophils and monocytes due to egg laying. In addition, the erythrocyte count and haemoglobin content significantly decreased in the middle of the laying period and then rebounded at the end of the laying period. The haematocrit content gradually decreased till the end of the laying period. All together, the results of this study underline the impact of the reproduction status of pheasant hens on basic blood parameters. The biochemical and haematological values presented in this study may be of help in assessing disease conditions in laying pheasant hens. PMID:24724469

Schumann, J; Bedanova, I; Voslarova, E; Hrabcakova, P; Chloupek, J; Pistekova, V

2014-01-01

54

Guideline: the laboratory diagnosis of malaria. General Haematology Task Force of the British Committee for Standards in Haematology.  

PubMed

UK National External Quality Assessment Service surveys indicate continuing problems in malaria diagnosis: inaccurate calculation of parasitaemia or failure to estimate it altogether, difficulty distinguishing Plasmodium vivax from P. ovale, reporting malaria parasites when none were present and misidentification of P. falciparum as another species still occur. Therefore, the British Committee for Standards in Haematology Guidelines for the Laboratory Diagnosis of Malaria have been revised. They are intended for use in the UK but may also prove useful in other non-endemic areas. Routine use of thick and thin films is advised for malaria diagnosis. Thick films should be stained using Giemsa or Field stain. Thin films should be stained with Giemsa stain or Leishman stain. Thick films should be examined by two observers, each viewing a minimum of 200 high power fields. If thick films are positive, the species should be determined by examination of a thin film. In the case of P. falciparum or P. knowlesi infection, the percentage of parasitized cells or the number of parasites per microlitre (/?l) should be estimated and reported. Rapid diagnostic tests (RDTs) for malarial antigen cannot replace microscopy but are indicated as a supplementary test when malaria diagnosis is performed by relatively inexperienced staff. Malaria RDTs are negative in babesiosis. PMID:24219330

Bailey, J Wendi; Williams, John; Bain, Barbara J; Parker-Williams, John; Chiodini, Peter L

2013-12-01

55

Effects of constant light on haematological parameters of cultured rainbow trout ( Oncorhynchus mykiss ) in the Southern Hemisphere  

Microsoft Academic Search

The effects of artificial photoperiod regimes on reproductive patterns have been studied in several species, as have haematological parameters. However, information on how artificial photoperiods may affect blood components is scarce, especially under field conditions. We have assessed the effects of constant light [long day (LD) photoperiod: 24 h (light):0 h (dark)] on haematological parameters of cultured rainbow trout in Chile

Ariel E. Valenzuela; Victor M. Silva; Alfredo E. Klempau

2006-01-01

56

Support vector machine versus logistic regression modeling for prediction of hospital mortality in critically ill patients with haematological malignancies  

Microsoft Academic Search

BACKGROUND: Several models for mortality prediction have been constructed for critically ill patients with haematological malignancies in recent years. These models have proven to be equally or more accurate in predicting hospital mortality in patients with haematological malignancies than ICU severity of illness scores such as the APACHE II or SAPS II 1. The objective of this study is to

T Verplancke; S Van Looy; D Benoit; S Vansteelandt; P Depuydt; F De Turck; J Decruyenaere

2008-01-01

57

Basic haematological values in carnivores--II. The Felidae.  

PubMed

1. Basic haematological values in 34 animals of eight carnivorous species are reported. 2. In four Northern lynxs (Lynx lynx lynx), two male and two female animals, the mean values are given: erythrocyte counts 8.51 X 10(12)/l, haematocrit 0.392/l, haemoglobin content 148.0 g/l and leukocyte count 7.92 X 10(9)/l. 3. In six male pumas (Puma concolor missolensis) the mean values estimated are: erythrocyte count 9.35 X 10(12)/l, haematocrit 0.43/l, haemoglobin content 163.9 g/l and leukocyte count 7.73 X 10(9)/l. Individual values in one female puma are also given. 4. In six jaguars (Panthera onca), three male and three female animals, the mean values are given: erythrocyte count 8.27 X 10(12)/l, haematocrit 0.37/l, haemoglobin content 137.1 g/l and leukocyte count 15.15 X 10(9)/l. 5. Only individual values are reported in one clouded leopard (Neofelis nebulosa), in one leopard (Panthera pardus saxicolor), in one Corbett's tiger (Panthera tigris Corbetti) and in one Altaic tiger (Panthera tigris Altaica). 6. In four lions (Panthera leo leo), two male and two female animals, the mean estimated values are: erythrocyte count 10.14 X 10(12)/l, haematocrit 0.462/l, haemoglobin content 159.0 g/l and leukocyte count 11.05 X 10(9)/l. In six female cheetahs (Acinonox jubatus jubatus) the mean values estimated are: erythrocyte count 7.86 X 10(12)/l, haematocrit 0.373/l, haemoglobin content 142.8 g/l and leukocyte count 8.65 X 10(9)/l. For three male cheetahs only individual values are reported. 8. All results achieved are compared with those abstracted from the literature and discussed. PMID:2886279

Pospísil, J; Kase, F; Váhala, J

1987-01-01

58

Clinical biochemistry, haematology and body weight in piglets.  

PubMed

Reference ranges for clinical biochemical parameters commonly investigated in pigs were determined in one- (day 1), 21- and 35-day old piglets. The mean and standard deviation were also estimated for body weight, and haematological and clinical biochemical parameters at these ages. The piglets were divided into 2 investigation groups according to whether they had a haemoglobin concentration < or = 80 g/l ("anaemic group") or > 80 g/l ("normal group") on days 14, 21 and 28. The "anaemic group" was compared to the "normal group" on days 21 and 35. Many of the clinical biochemical parameters varied according to age. Some of the enzymes had high average values and wide reference ranges in piglets, especially on day 1, compared to the reference ranges for sows given in the literature. The reference ranges for some of the metabolic parameters were broader on day 1 than later in the preweaning period. The reference ranges for albumin, total iron-binding capacity and serum iron were, however, lower and more narrow on day 1. On days 21 and 35, relatively high values for phosphorus must be considered "normal" compared to the figures given in the literature for adult pigs. The other minerals seemed to be quite unaffected of age, but some were affected by anaemia. The anaemic piglets had lower average serum iron but higher total iron-binding capacity than the "normal" piglets on days 21 and 35. However, variation between piglets gave wide reference ranges, indicating that these parameters will only have limited usefulness in detecting iron deficiency anaemia in piglets. The electrolytes seemed also to be affected by the existence of anaemia. The body weight and leukocyte counts were significantly lower in the "anaemic group" than the "normal group" on day 35, while the greatest differences in clinical biochemical parameters between the groups were found on day 21, when the piglets in the "anaemic group" were most severely anaemic. Although these piglets suffered from severe iron-deficiency anaemia, only a few clinical biochemical parameters were affected, and the differences between groups were mostly small. PMID:9787501

Egeli, A K; Framstad, T; Morberg, H

1998-01-01

59

Distinct haematological disorder with deletion of long arm of No. 5 chromosome  

Microsoft Academic Search

So far, the only specific chromosome abnormality in haematological disorders is the Ph1-chromosome. Chromosome abnormalities have been described in so-called idiopathic side-roblastic or refractory anaemias1 but a constant cytogenetic abnormality associated with a defined clinical syndrome has not been reported.

Herman van den Berghe; Jean-Jacques Cassiman; Guido David; Jean-Pierre Fryns; JEAN-LOUIS MICHAUX; GERARD SOKAL

1974-01-01

60

A cluster of suspected Pneumocystis carinii Pneumonia following intensive chemotherapy in a Belfast haematology unit.  

PubMed Central

Five cases of pneumocystis carinii pneumonia were diagnosed in adult patients following intensive chemotherapy in the Royal Group of Hospitals haematology unit, Belfast, within a space of six months. The common features and the risk factors contributing to the increased susceptibility of these patients are discussed, as are the likely mechanisms of transmission of infection.

Ong, Y. L.; Jones, F. G.

1998-01-01

61

Relationship between environmental fungal contamination and the incidence of invasive aspergillosis in haematology patients  

Microsoft Academic Search

Invasive aspergillosis (IA) is a major opportunistic infection in haematology patients. Spore inhalation is the usual route of Aspergillus infection, suggesting a determining role of environmental contamination by spores in the epidemiology of IA. We prospectively examined the relationship between environmental contamination by Aspergillus and other fungal species and the incidence of invasive nosocomial aspergillosis (INA) in a bone marrow

C. Alberti; A. Bouakline; P. Ribaud; C. Lacroix; P. Rousselot; T. Leblanc; F. Derouin

2001-01-01

62

Impact of Plasmodium falciparum infection on haematological parameters in children living in Western Kenya  

Microsoft Academic Search

BACKGROUND: Malaria is the commonest cause of childhood morbidity in Western Kenya with varied heamatological consequences. The t study sought to elucidate the haemotological changes in children infected with malaria and their impact on improved diagnosis and therapy of childhood malaria. METHODS: Haematological parameters in 961 children, including 523 malaria-infected and 438 non-malaria infected, living in Kisumu West District, an

Robert N Maina; Douglas Walsh; Charla Gaddy; Gordon Hongo; John Waitumbi; Lucas Otieno; David Jones; Bernhards R Ogutu

2010-01-01

63

Prevalence of rheumatic manifestations and antineutrophil cytoplasmic antibodies in haematological malignancies. A prospective study  

Microsoft Academic Search

Objective. To evaluate the prevalence of antineutrophil cytoplasmic antibodies (ANCA) and rheumatic manifestations associated with chronic haematological malignancies. Methods. Two groups of patients were prospectively studied (group I: 60 patients with myelodysplastic syndromes and group II: 140 patients with lymphoid malignancies) for clinical 'immune' manifestations and ANCA. Results. In the myelodysplastic group, six patients had ANCA-negative systemic medium- size vasculitis,

M. A. Hamidou; S. Derenne; M. A. P. Audrain; J. M. Berthelot; A. Boumalassa; J. Y. Grolleau

2000-01-01

64

Haematological response of curimbas Prochilodus lineatus, naturally infected with Neoechinorhynchus curemai.  

PubMed

This study evaluated the haematological response of curimbas Prochilodus lineatus, naturally infected with Neoechinorhynchus curemai (Acanthocephala: Neoechinorhynchidae). Thirty-seven fish were captured in October 2010 from the Mogi Guaçu River, Porto Ferreira, SP, Brazil. Infected fish presented increased mean corpuscular volume of erythrocytes, and lower thrombocyte and higher monocyte counts than uninfected fish. PMID:23557315

Belo, M A A; Souza, D G F; Faria, V P; Prado, E J R; Moraes, F R; Onaka, E M

2013-04-01

65

Incidence of haematological malignancies in French Polynesia between 1990 and 1995  

Microsoft Academic Search

To determine the incidence of haematological malignancies in French Polynesia from 1990 to 1995, we collected cases from the local cancer registry, sanitary evacuation files and all the histopathology and clinical biology laboratories. All leukaemias, non Hodgkin’s lymphomas, and multiple myelomas incidence was slightly lower among French Polynesians than among Maoris from New-Zealand and Hawaiians of Hawaii. Standardised Incidence Ratio

Laurent Roda; Florent de Vathaire; Bernard Rio; Agnes Le Tourneau; Patrice Petididier; François Laudon; Robert Zittoun

1999-01-01

66

Oncology\\/haematology nurses: a study of job satisfaction, burnout, and intention to leave the specialty  

Microsoft Academic Search

The impact of the current nursing shortage on the health care system is receiving attention by both state and federal governments. This study, using a convenience sample of 243 oncology\\/haematology nurses working in 11 Queensland health care facilities, explored factors that influence the quality of nurses' working lives. Although nurses reported high levels of personal satisfaction and personal accomplishment, results

Linda Barrett; Patsy Yates

2002-01-01

67

PCR as a screening test for invasive aspergillosis in haematological patients: a pilot study.  

PubMed

Invasive aspergillosis is a leading cause of morbidity and mortality in immunocompromised patients, particularly in individuals with haematological malignancy and in haematopoietic stem cell transplant recipients. Nowadays, the galactomannan (GM) assay has been widely used as an indication of invasive aspergillosis, even though the test is known to generate false-positive results. The aim of this study was to compare the performance of GM and real-time PCR (qPCR) to detected Aspergillus in blood samples obtained from high-risk haematological patients. Haematological patients were screened twice weekly with GM testing, which was performed by the Platelia ELISA kit. An additional sample of whole blood (4 ml) was obtained for the purpose of qPCR testing. Sixty-four samples from 12 patients with haematopoietic stem cell transplant or haematological malignancy were studied. The overall accordance between GM and qPCR tests was 96.9 % (62 samples). Only two samples showed contradictory results, with positive GM test and negative real-time PCR results. Based on the high concordance between GM and qPCR in terms of negative results, the main utility of qPCR could be in the confirmation of positive results seen with GM testing. PMID:24309908

da Silva, Thomas Victor Maciel; Carneiro, Lilian Carla; Ramos, Francine dos Santos; Baethgen, Ludmila Fiorenzano; Paz, Alessandra Aparecida; Larentis, Daniela Zilio; Daudt, Liane Esteves; Tusset, Cintia; Lopes-Bezerra, Leila Maria; Pasqualotto, Alessandro C

2014-02-01

68

Toxic effects of some plants in the genus Euphorbia on haematological and biochemical parameters of rats  

Microsoft Academic Search

ADEDAPO, A. A., M. O. ABATAN, O. O. OLORUNSOGO: Toxic effects of some plants in the genus Euphorbia on haematological and biochemical parameters of rats. Vet. arhiv 74, 53-62, 2004. ABSTRACT The toxic effects of 5 suspected poisonous plants of the genus Euphorbia (Euphorbia balsamifera Aiton, E. heterophylla L., E. hirta L., E. hyssopifolia L., and E. lateriflora Schum and

Adeolu A. Adedapo; Matthew O. Abatan; Olufunso O. Olorunsogo

2004-01-01

69

Changes of the immunological and haematological parameters in rabbits after bendiocarbamate application  

Microsoft Academic Search

The effect of bendiocarbamate application (5 mg\\/kg b.w.) on the haematological and immunological parameters in rabbits was evaluated. Total leukocyte cell count, erythrocyte cell count, differential cell count were determined during the period of three months of bendiocarbamate application and compared with those in healthy animals. The immunotoxic effect was evaluated by the test of ingestion ability of phagocytes (phagocytic

Jana Mojzisova; Peter Massányi; Jan Danko; Alexandra Trbolova; Eva Petrovova; David Mazensky; Katarina Vdoviakova; Lenka Luptakova; Norbert Torma

2012-01-01

70

Haematological analyses in rainbow trout Oncorhynchus mykiss affected by viral haemorrhagic septicaemia (VHS)  

Microsoft Academic Search

Rainbow trout Oncorhynchus mykiss weighing 87 ± 15 g (mean ± SD) were infected with viral haemorrhagic septicaemia virus (VHSV) and the haematological and biochemical profiles of peripheral blood examined. Depending on the clinical signs and gross pathology, the fish were divided into 2 groups: Group A included fish in the acute stage, Group B comprised fish in the chronic

2003-01-01

71

Haematological and spermatotoxic effects of ethylene glycol monomethyl ether in copper clad laminate factories  

Microsoft Academic Search

OBJECTIVESTo investigate the effects of ethylene glycol monomethyl ether (EGME) on haematology and reproduction in exposed workers.METHODS53 Impregnation workers from two factories that make copper clad laminate with EGME as a solvent were recruited as the exposed group. Another group of 121 lamination workers with indirect exposure to EGME was recruited as the control group. Environmental monitoring of concentrations of

Tung-Sheng Shih; An-Tsz Hsieh; Guo-Dong Liao; Yeong-Hwang Chen; Saou-Hsing Liou

2000-01-01

72

Risk factors for pneumothorax during percutaneous hickman line insertion in patients with solid and haematological tumours  

Microsoft Academic Search

The purpose of this study was to identify risk factors for pneumothorax during percutaneous subclavian Hickman line insertion in patients with haematological (HT) or solid tumours (ST). One hundred and twelve patients (55 HT, 57 ST) had 132 subclavian Hickman lines inserted under fluoroscopic control. Lines were inserted on the left on 116 occasions and the right in 16. Thirty-five

K. J. Harrington; H. S. Pandha; J. S. Hollyer; S. A. Kelly; A. R. Bateman; C. Lewanski; P. Morris; J. E. Jackson

1995-01-01

73

Trypanosoma evansi infection and the influence of some hormones on haematological indices in albino rats  

Microsoft Academic Search

Changes in the haematological indices like haemoglobin, mean corpuscular haemoglobin, colour index, iron, total red blood\\u000a corpuscles, white blood corpuscles and blood glucose in albino rats duringTrypanosoma evansi infection and the influence of some hormones like hydrocortisone, insulin and thyroxine on these changes have been investigated.

M Lemalatha; L Narasimha Rao; M Kameswari

1986-01-01

74

Haematologic determinants of left atrial spontaneous echo contrast in mitral stenosis  

Microsoft Academic Search

Objectives: To determine if a relationship exists in mitral stenosis, in patients with either sinus rhythm or atrial fibrillation, between left atrial spontaneous echo contrast and the haematologic indices haematocrit, red cell concentration, mean corpuscular volume, platelet count and volume. Methods: Left atrial spontaneous echo contrast severity was graded on a scale of 0–4 in 163 patients with symptomatic mitral

Roger E Peverill; Richard Graham; John Gelman; Lynette A Yates; Richard W Harper; Joseph J Smolich

2001-01-01

75

Malaria Parasitemia Associated with Febrile Neutropenia in African Patients Undergoing Chemotherapy for Haematological Malignancies  

Microsoft Academic Search

Background: Very little is documented in the medical literature on the association between malaria parasitemia and febrile neutropenia in patients undergoing cancer chemotherapy. Methods: This report will concentrate on the clinical presentation and outcome of 3 patients with haematological malignancies undergoing chemotherapy who developed febrile neutropenia and malaria parasitemia concurrently. Results: Three patients infected with documented malaria during a febrile

Bernardo L. Rapoport; Almarie Uys

2008-01-01

76

Haematological characteristics predicting susceptibility for ascites. 1. High carbon dioxide tensions in juvenile chickens  

Microsoft Academic Search

1. Male broilers of two different genetic stocks, a pure broiler sire line (A) and commercially available Ross broilers (B), were used to study the effect of haematological characteristics in juvenile chickens on the development of clinical ascitic signs. Production performance (body weight gain (BWG), feed intake (FI), feed conversion ratio (FCR)) from 448 birds per stock was measured from

C. W. Scheele; J. D. Van Der Klis; C. Kwakernaak; N. Buys; E. Decuypere

2003-01-01

77

Haematological changes in active chronic hepatitis with reference to the role of the spleen  

Microsoft Academic Search

The haematological role of the spleen has been investigated in a series of 22 patients with active chronic hepatitis. Severe pancytopenia occurred in one patient after three years of steroid therapy and this episode was associated with an increase in spleen size and a high splenic index of red cell destruction. Although the spleen was usually enlarged in the remainder

P J Toghill; S Green

1975-01-01

78

Effect of malarial infection on haematological parameters in population near Thailand-Myanmar border  

PubMed Central

Background Malaria is a major mosquito-borne public health problem in Thailand with varied haematological consequences. The study sought to elucidate the haematological changes in people who suspected malaria infection and their possible predictive values of malaria infection. Methods Haematological parameters of 4,985 patients, including 703 malaria-infected and 4,282 non-malaria infected, who admitted at Phop Phra Hospital, Tak Province, an area of malaria endemic transmission in Thailand during 2009 were evaluated. Results The following parameters were significantly lower in malaria-infected patients; red blood cells (RBCs) count, haemoglobin (Hb), platelets count, white blood cells (WBCs) count, neutrophil, monocyte, lymphocyte and eosinophil counts, while mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), Mean corpuscular haemoglobin concentration (MCHC), neutrophil-lymphocyte ratio (NLR), and monocyte-lymphocyte ratio (MLR) were higher in comparison to non-malaria infected patients. Patients with platelet counts?haematological parameters with low platelet, WBCs, and lymphocyte counts being the most important predictors of malaria infection. When used in combination with other clinical and microscopy methods, these parameters could improve malaria diagnosis and treatment.

2014-01-01

79

mTOR Inhibitors and Their Potential Role in Therapy in Leukemia and Other Haematologic Malignancies  

PubMed Central

Summary The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that functions as a key regulator of cell growth, protein synthesis, and cell-cycle progression through interactions with a number of signaling pathways, including PI3K/AKT, ras, TCL1, and BCR/ABL. Many haematologic malignancies have aberrant activation of the mTOR and related signaling pathways. Accordingly, mTOR inhibitors, a class of signal transduction inhibitors that were originally developed as immunosuppressive agents, are being investigated in preclinical models and clinical trials for a number of haematologic malignancies. Sirolimus and second generation mTOR inhibitors such as temsirolimus and everolimus, are safe and relatively well-tolerated, making them potentially attractive as single agents or in combination with conventional cytotoxics and other targeted therapies. Promising early clinical data suggests activity of mTOR inhibitors in a number of haematologic diseases, including acute lymphoblastic leukemia, chronic myelogenous leukemia, mantle cell lymphoma, anaplastic large cell lymphoma, and lymphoproliferative disorders. This review describes the rationale for using mTOR inhibitors in a variety of haematologic diseases with a focus on their use in leukemia.

Teachey, David T.; Grupp, Stephan A.; Brown, Valerie I.

2009-01-01

80

Risk of disability pension for patients diagnosed with haematological malignancies: A register-based cohort study.  

PubMed

Abstract Patients with haematological malignancies are at increased risk of experiencing work-related problems. The aims of this study were to compare the risk of disability pension (DP) among patients diagnosed with eight subtypes of haematological malignancies to a reference cohort, and to determine if relative risks differ between these subtypes; to evaluate the influence of socioeconomic factors, demographic factors, and clinical factors on the risk of DP; and to investigate if these associations differ between the reference cohort and the patient cohort. Material and methods. We combined data from national registers on Danish patients diagnosed with haematological malignancies between 2000 and 2007 and a reference cohort without a history of these diseases. A total of 3194 patients and 28 627 reference individuals were followed until DP, emigration, old age pension or anticipatory pension, death or 26 February 2012, whichever came first. Results. A total of 550 (17%) patients and 1511 (5%) reference individuals were granted DP. Age- and gender-adjusted relative risks differed significantly between the subgroups of haematological malignancies and ranged from 2.64 (95% CI 1.84-3.78) for patients with Hodgkin lymphoma to 12.53 (95% CI 10.57-14.85) for patients with multiple myeloma. In the patient cohort we found that gender, age, comorbidity, ethnicity, educational level, household income, history of long-term sick leave, and need of treatment with anxiolytics or antidepressants after diagnosis were associated with receiving DP. However, most of these associations were stronger in the reference cohort. Conclusion. All eight subtypes of haematological malignancies were associated with an increased risk of DP compared to the reference cohort. The relative risks differed according to subtype, and patients with multiple myeloma had the highest risk of DP. Furthermore, most socioeconomic, demographic and clinical factors had a stronger impact on the risk of DP in the reference cohort than in the patient cohort. PMID:24456498

Horsboel, Trine A; Nielsen, Claus V; Andersen, Niels T; Nielsen, Bendt; de Thurah, Annette

2014-06-01

81

Serious haematological toxicity during and after ipilimumab treatment: a case series  

PubMed Central

Introduction Immunotherapy with the anti-cytotoxic T-lymphocyte antigen-4 monoclonal antibody ipilimumab has been shown to improve overall survival in previously treated and treatment-naïve patients with unresectable stage III or IV melanoma. Consistent with its proposed immunomodulating mechanism of action, the most common toxicities associated with ipilimumab therapy are immune-related in nature and include those related to the skin and gastrointestinal tract, with endocrine and hepatic events also frequent. Other rare adverse events, including haematological aberrations, may also occur and can have serious consequences if unrecognised. Here we describe three patients who developed serious haematological adverse events during or after treatment with ipilimumab. Case presentation Three Caucasian patients (two women aged 68 and 49 years and one man aged 70 years) with metastatic melanoma experienced anaemia and/or leukopenia (neutropenia) with toxicity of various grades during or after treatment with ipilimumab, without significant changes to other haematological values. Two of the patients stopped treatment after the third ipilimumab dose, one because of severe anaemia that required blood transfusion and the other due to febrile neutropenia that was treated with antibiotics and granulocyte-macrophage colony-stimulating factor stimulation. The third patient developed anaemia and leukopenia after treatment during the follow-up period. The results of autoimmunity tests performed were positive and corticosteroids were used to treat these events as per side-effects treatment algorithms specifically developed for the management of immune-related adverse events associated with ipilimumab, an approach that was safe and effective. Conclusions Haematological toxicity is a rare but potentially serious immune-related side effect of ipilimumab therapy. However, if promptly recognised and treated, haematological toxicity is manageable and can be reversed with standard corticosteroid treatment as recommended for other ipilimumab immune-related side effects.

2014-01-01

82

Biochemical and haematological changes in HIV subjects receiving winniecure antiretroviral drug in Nigeria  

PubMed Central

Background Hematological and biochemical abnormalities are among the most common clinicopathological manifestations of HIV patients on ART. Consequently, the development and assessment of indigenous antiretroviral drugs with minimal abnormalities becomes a necessity. The objective of this investigation was to assess potential haematological and biochemical abnormalities that may be associated with the administration of Winniecure ART in HIV patients undergoing treatment in Nigeria. Fifty (50) confirmed HIV positive ART naïve patients aged 36?±?10 were observed for haematological and biochemical responses for 12 weeks. Haematological responses were assessed thrice at 6 weeks interval using coulter Ac-T differential analyser and biochemical indicators (bilirubin, creatine, urea, amylase, ALT, ALP, AST, albumin) assayed spectrophotometrically. Results The biochemical parameters ALP (P??0.002). Haematological results showed consistent reduction of ESR, eosinophil, absolute and differential lymphocytes, granulocytes and total WBC in the test subjects throughout the assessment period. Conversely, haemoglobin, platelet and PCV increased significantly (P?haematological abnormalities and normal kidney function was unaffected though there were signs of possible abnormal levels of hepatic enzymes beyond 12 weeks of treatment.

2013-01-01

83

Evaluation of the Sysmex XT-2000iV haematology analyser for rat, dog and mouse whole blood samples  

Microsoft Academic Search

The Sysmex XT-2000iV is a dedicated haematology analyser with full blood count, reticulocyte and five-population differential\\u000a leucocyte capabilities for several laboratory animal species. The laboratory animal species chosen for this study were rat,\\u000a dog and mouse. Results from dipotassium ethylenediamine tetraacetic acid (K2EDTA) samples from these species were compared with the laboratory’s established haematology analyser, the Bayer (now Siemens)\\u000a Advia

R. A. Mathers; G. O. Evans; J. Bleby; T. Tornow

2008-01-01

84

Factors influencing haematological recovery after allogeneic bone marrow transplantation in leukaemia patients treated with methotrexate-containing GVHD prophylaxis  

Microsoft Academic Search

In the present single institution study of 66 leukaemia patients (28 AML, 23 ALL, 15 CML), the factors influencing haematological\\u000a recovery after allogeneic bone marrow transplantation (alloBMT) were analysed retrospectively to identify the optimal conditions\\u000a required for rapid haematological recovery after alloBMT. All patients received GVHD prophylaxis with cyclosporine A plus\\u000a methotrexate. The mean number of days required to achieve

H. T. Hassan; C. Krog; M. Stockschläder; W. Zeller; W. Krüger; R. Erttmann; A. R. Zander

1997-01-01

85

“I was never like that”: Australian findings on the psychological and psychiatric sequelae of corticosteroids in haematology treatments  

Microsoft Academic Search

Goals of work  Corticosteroid treatments have been well documented to cause severe emotional and even psychiatric disturbances. Despite that\\u000a corticosteroid use is at the core of most treatment protocols for haematological malignancies, there is a dearth of published\\u000a research (and controversy in the existing research) on the emotional and psychiatric sequelae of corticosteroid use for haematology\\u000a patients and its connection with

Pam McGrath; Mary Anne Patton; Sarah James

2009-01-01

86

Influence of zinc on cadmium induced haematological and biochemical responses in a freshwater teleost fish Catla catla  

Microsoft Academic Search

The haematological (Hb, RBC, WBC) and biochemical (protein and glucose) profiles of a freshwater fish Catla catla were studied under sub-lethal toxicity of cadmium chloride for 25 days (Treatment I). In addition, the influence of zinc\\u000a on cadmium toxicity was investigated for haematological and biochemical parameters (Treatment II). In both the experiments,\\u000a blood haemoglobin and plasma protein level decreased in the

Sweety R. Remyla; Mathan Ramesh; Kenneth S. Sajwan; Kurunthachalam Senthil Kumar

2008-01-01

87

Haematological responses to exposure to sublethal concentration of cadmium in air breathing fish, Channa punctatus (Bloch).  

PubMed

The effect of sublethal concentration (29 mg/l) of cadmium on haematological parameters of Channa punctatus after the exposure periods of 7, 15 and 30 days were studied. In treated groups of cadmium exposed fish have been found to significantly decreased in total erythrocyte count, haemoglobin content, packed cell volume, mean corpuscular haemoglobin concentration and oxygen carrying capacity of blood, and at the same time the white blood cell count, mean cell volume and mean corpuscular haemoglobin were found to significantly increased. Both increasing and decreasing of the above mentioned haematological characteristics of blood cells of cadmium intoxicant are mainly with time dependent effects. With regard to the pathological characteristics of blood cells of cadmium intoxicant fish shows fragility and rupture of erythrocytic membrane and leads to haemolysis, which are very severe on 30 day of exposure group. PMID:16114472

Karuppasamy, R; Subathra, S; Puvaneswari, S

2005-01-01

88

Incidence of haematological malignancies in French Polynesia between 1990 and 1995.  

PubMed

To determine the incidence of haematological malignancies in French Polynesia from 1990 to 1995, we collected cases from the local cancer registry, sanitary evacuation files and all the histopathology and clinical biology laboratories. All leukaemias, non Hodgkin's lymphomas, and multiple myelomas incidence was slightly lower among French Polynesians than among Maoris from New-Zealand and Hawaiians of Hawaii. Standardised Incidence Ratio (SIR) for Hodgkin's disease among females was 0.08 when comparing to Hawaiians and 0.33 when comparing to Maoris. Other salient features were a high proportion of high grade and Burkitt's lymphoma, the absence of Hodgkin's disease after 40 years of age, a low incidence of chronic lymphoid leukaemia, and a high non lymphoblastic/lymphoblastic acute leukaemia ratio in childhood. This study stresses the peculiar incidence of some haematological malignancies in this south pacific area. PMID:10229320

Roda, L; de Vathaire, F; Rio, B; Le Tourneau, A; Petididier, P; Laudon, F; Zittoun, R

1999-04-01

89

Normal haematology and blood biochemistry of wild Nile crocodiles (Crocodylus niloticus) in the Okavango Delta, Botswana.  

PubMed

Wild Nile crocodiles (Crocodylus niloticus) of various size classes were captured in the Okavango Delta, Botswana. Blood was collected from the post occipital sinus and used for the determination of a wide range of haematological and biochemical parameters. These values were compared between the sexes and between 3 size classes. The values were also compared with the limited data available from farmed Nile crocodiles, as well as from other wild Nile crocodiles. The Okavango crocodiles were comparatively anaemic, and had comparatively low total protein and blood glucose levels. There was a high prevalence of Hepatozoon pettiti infection, however, there was no significant difference in haematological values between the infected and uninfected crocodiles. The values reported here will be useful in diagnostic investigations in both zoo and farmed Nile crocodiles. PMID:18237036

Lovely, C J; Pittman, J M; Leslie, A J

2007-09-01

90

Haematological and serological data from dogs raised worm-free and monospecifically infected with helminths.  

PubMed

Pups free from helminth infection were obtained by treating the bitch prior to parturition, and the bitch and her pups after whelping, with oxfendazole and praziquantel. They were then housed in an area free from contamination with helminth eggs. Freedom from infection was confirmed by necropsy examinations and by serological tests using the Enzyme Linked Immunosorbent Assay (ELISA). Groups of pups were infected with Toxocara canis, Echinococcus granulosus, Taenia ovis, T. pisiformis and T. hydatigena and bled at 5-day intervals. Haematological examinations and measurement of serum proteins were performed on blood samples obtained throughout 30 days of infection. Levels of serum proteins were not significantly altered in any infection. Pups infected with T. canis showed a marked increase in numbers of circulating eosinophils during the phase of larval migration through the liver and lungs; they also showed marked antibody responses to T. canis larval ES antigen. Haematological values were not significantly altered in any other pups. PMID:6525114

Jenkins, D J; Rickard, M D

1984-10-01

91

Non-malignant haematology research in the UK: looking forward to new opportunities.  

PubMed

Over the last few years there has been rapid and radical change in the way clinical research in the UK is funded and supported within the NHS. This has resulted from restructuring and major new investment in research infrastructure, co-ordinated through Clinical Local Research Networks (CLRNs) and equivalent organisations in the devolved nations. CLRNs have resources to support local researchers undertake studies that have been adopted on to the national research portfolio. For example, CLRNs can help with gaining local approvals or provide research nurses to recruit patients, undertake study procedures and perform data entry. CLRNs can establish Local Speciality Groups in a number of areas of medicine, including nonmalignant haematology. These new networks offer non-malignant haematology access to significant new resources and a major opportunity to support clinical research for the benefit of our patients. PMID:21105240

Collins, P W; Baglin, T P; Dang, R; Evans, G; Greaves, M; Laffan, M; Pasi, K J; Rose, P; Stanworth, S; Toh, C H

2010-09-01

92

Studies on experimental Jembrana disease in Bali cattle. II. Clinical signs and haematological changes.  

PubMed

The clinical and haematological changes which occurred in 18 Bali cattle (Bos javanicus) experimentally infected with Jembrana disease are described. The major clinical signs were an elevated rectal body temperature persisting for 7 days (range 5 to 12 days), lethargy, anorexia, enlargement of the superficial lymph nodes, a mild ocular and nasal discharge, diarrhoea with blood in the faeces and pallor of the mucous membranes. Not all of these changes occurred in all affected cattle. The major haematological changes included leucopenia, lymphopenia, eosinopenia and a slight neutropenia, a mild thrombocytopenia, a normocytic normochromic anaemia, elevated blood urea concentrations and reduced total plasma protein. The mortality rate in the experimentally infected cattle was 17 per cent. The similarity of Jembrana disease to malignant catarrhal fever and to diseases of cattle associated with Ehrlichia is discussed. PMID:2394847

Soesanto, M; Soeharsono, S; Budiantono, A; Sulistyana, K; Tenaya, M; Wilcox, G E

1990-07-01

93

A data management software for the Sysmex NE 8000 haematology analyser  

Microsoft Academic Search

The Sysmex NE 8000 (TOA-Japan) is a haematology analyser that performs blood cells count and leukocyte differential count. For facilitating the work of technical validation, we developed a software adapted to any IBM® or compatible PC running under MS-DOS®, to manage the analyser. Data are automatically collected via the RS-232 interface from the analyser or keyed in for the other

J. P. Cambus; F. Nguyen; F. Cambus

1996-01-01

94

Haematological Alterations Observed in Equine Routine Complete Blood Counts. A Retrospective Investigation  

Microsoft Academic Search

:   Results on more than 1000 complete blood counts (CBC) performed on hot-blooded horses, including Thoroughbred, Standardbred,\\u000a and Arabian, have been reviewed. All the data were processed and stored at the Laboratory of Haematology, Department Veterinary\\u000a Clinic, University of Pisa, over the last 2 years, and involved samples withdrawn from both inpatients and outpatients of\\u000a the clinic.\\u000a \\u000a ?The CBC is

A. GAVAZZA; A. J. DELGADILLO; B. GUGLIUCCI; A. PASQUINI; G. LUBAS

2002-01-01

95

Outbreak of cutaneous zygomycosis associated with the use of adhesive tape in haematology patients.  

PubMed

We report an outbreak of cutaneous Rhizopus oryzae infection associated with adhesive polyethylene tapes used to stabilize peripheral venous catheters in four patients. All patients were suffering from haematological diseases; the infection severity was proportional to the duration of neutropenia. Intervention with systemic antifungal treatment and surgical debridement was required for resolution of the infection. The entire batch of tapes was withdrawn and the outbreak subsided. PMID:22633275

Lalayanni, C; Baliakas, P; Xochelli, A; Apostolou, C; Arabatzis, M; Velegraki, A; Anagnostopoulos, A

2012-07-01

96

Selected haematological and plasma chemistry parameters in juvenile and adult degus (Octodon degus)  

Microsoft Academic Search

Thirty-five juvenile (mean age 6.3 weeks) and 35 adult (mean age 2.0 years) healthy degus (Octodon degus) were studied to investigate selected haematological and plasma biochemistry parameters. Animals were anaesthetised with isoflurane, and blood was withdrawn from the cranial vena cava. Erythrocyte, haematocrit and neutrophil counts (including the percentage of neutrophils) were significantly higher in the adult degus than in

V. Jekl; K. Hauptman; E. Jeklova; Z. Knotek

2011-01-01

97

A study on tuberculosis in buffaloes: some epidemiological aspects, along with haematological and serum protein changes  

Microsoft Academic Search

The study was conducted to ascertain the epidemiology, together with effects of bovine tuberculosis, on certain haematological parameters and serum proteins at two Livestock Experiment Stations in Pakistan. The results on prevalence of tuberculosis in buffaloes on the basis of comparative intradermal tuberculin test revealed it to be from as high as 8.48% (14\\/165) to as low as 2.45% (4\\/163)

Muhammad Tariq Javed; Mahmood Usman; Muhammad Irfan; Monica Cagiola

2006-01-01

98

Haematological reference values for adult pumas, lions, tigers, leopards, jaguars and cheetahs.  

PubMed

Normal haematological values and fibrinogen levels were obtained from a number of healthy adult Felidae in the collection of the Zoological Society of London. The group comprised 29 pumas (Felis concolor), 32 lions (Panthera leo), 27 tigers (P tigris), 19 leopards (P pardus), 18 jaguars (P onca) and 22 cheetahs (Acinonyx jubatus). The values provided a basis for identifying abnormalities in the blood of sick individuals of these species and for undertaking interspecies comparisons. PMID:3775118

Hawkey, C M; Hart, M G

1986-09-01

99

Voriconazole associated torsades de pointes in two adult patients with haematological malignancies  

PubMed Central

Voriconazole can prolong the QT interval contributing to life-threatening cardiac arrhythmia. Torsades de pointes is an uncommon but serious complication of voriconazole use which may be under-recognised. We present torsades de pointes in two patients with underlying haematological malignancy being treated for invasive fungal infection with voriconazole. Patients receiving voriconazole should be screened and monitored for evidence of QT prolongation, and if prolongation detected, consideration given to alternative treatments or more intensive cardiac monitoring.

Brown, Jeremy D.; Lim, Lyn-li; Koning, Sonia

2014-01-01

100

Comparison of haematological changes and strongyle faecal egg counts in donkeys in Kiambu district of Kenya  

Microsoft Academic Search

Haematological data were obtained through analysis of blood samples taken from sixteen donkeys randomly selected from a donkey population in Kiambu District, Kenya. Nine out of sixteen of the donkeys examined were anaemic, with low RBC counts (ranging between 3.2 - 4.18 x 106\\/ml) and low Hb concentrations (ranging between 6.6 - 9.7 gm%). Seven out of nine of these

A. K. Lew; T. A. Ngati; W. K. Munyua; N. E. Maingi

101

Changes in period and cohort effects on haematological cancer mortality in Spain, 1952-2006  

PubMed Central

Background In contrast to other haematological cancers, mortality from non-Hodgkin’s lymphoma and multiple myeloma increased dramatically during the second half of the 20th century in most developed countries. This widespread upward trend remains controversial, as it may be attributable either to progressive improvements in diagnosis and certification or to increasing exposures to little-known but relevant risk factors. Methods To assess the relative contribution of these factors, we analysed the independent effects of age, death period, and birth cohort on haematological cancer mortality rates in Spain across the period 1952-2006. Weighted joinpoint regression analyses were performed to detect and estimate changes in period and cohort curvatures. Results Although mortality rates were consistently higher among men, trends across periods and cohorts were virtually identical in both sexes. There was an early period trend reversal in the 1960s for Hodgkin’s disease and leukaemia, which was delayed to the 1980s for multiple myeloma and the 1990s for non-Hodgkin’s lymphoma. Birth cohort patterns showed a first downturn for generations born in the 1900s and 1910s for all haematological cancers, and a second trend reversal for more recent cohorts born in the 1950s and 1960s for non-Hodgkin’s lymphoma and leukaemia. Conclusions The sustained decline in Hodgkin’s disease mortality and the levelling off in leukaemia seem to be driven by an early period effect linked to improvements in disease treatment, whereas the steep upward trends in non-Hodgkin’s lymphoma and multiple myeloma mortality in Spain are more likely explained by a cohort effect linked to better diagnosis and death certification in the elderly. The consistent male excess mortality across all calendar periods and age groups points to the importance of possible sex-related genetic markers of susceptibility in haematological cancers.

2014-01-01

102

Experimental coronary vein obstruction in sheep: changes in haematological and inflammatory markers  

Microsoft Academic Search

It has been suggested that obstruction of coronary veins can induce myocardial infarction similar to coronary artery obstruction.\\u000a In this study, the coronary veins of the sheep heart were blocked experimentally and haematological and inflammatory indices\\u000a (haptoglobin, serum amyloid A, tumour necrosis factor-? and interferon-?) alterations were studied. Twenty sheep were used\\u000a for this study. Anaesthesia was induced by ketamine

Fatemeh Dehghani Nazhvani; M. B. Sharifkazemi; S. N. Dehghani; S. Nazifi; M. Shafa

103

Can food affect the bioavailability of chlorambucil in patients with haematological malignancies?  

Microsoft Academic Search

Pharmacokinetic studies in ten patients with haematological disorders were undertaken on the first and second days of one course of chemotherapy. Patients received chlorambucil under fasting and non-fasting conditions. Plasma concentrations of chlorambucil were determined by a reversed-phase high-performance liquid chromatography assay. Statistical analysis by the Wilcoxon signed rank test for non-parametric data indicated that food caused a significant reduction

C. G. Adair; J. M. Bridges; Z. R. Desai

1986-01-01

104

Allogeneic transplant outcomes are not affected by body mass index (BMI) in patients with haematological malignancies  

Microsoft Academic Search

Bone marrow transplantation is frequently used as a consolidation therapy in patients with haematological malignancies to\\u000a improve the outcome of these patients. Obese individuals have larger absolute lean body and fat masses than non-obese individuals\\u000a of the same age, gender and height, which might lead to altered pharmacokinetics of chemotherapeutic agents. Data on the impact\\u000a of body mass on transplant

Emmanouil Nikolousis; Sandeep Nagra; Shankara Paneesha; Julio Delgado; Kathy Holder; Lynn Bratby; Sridhar Chaganti; Richard Lovell; Donald Milligan

2010-01-01

105

Haematological characterization of loach Misgurnus anguillicaudatus: Comparison among diploid, triploid and tetraploid specimens  

Microsoft Academic Search

The purpose of this study was to determine whether diploid, triploid and tetraploid loach (Misgurnus anguillicaudatus) differed in terms of their main haematological and physiological characteristics. Diploid and tetraploid fish were produced by crossing of natural diploids (2n×2n) and natural tetraploids (4n×4n), respectively. Triploid fish were produced by hybridization between diploid males and tetraploid females. The blood cells were significantly

Zexia Gao; Weimin Wang; Khalid Abbas; Xiaoyun Zhou; Yi Yang; James S. Diana; Hanping Wang; Huanling Wang; Yang Li; Yuhua Sun

2007-01-01

106

Effects of iron glycine chelate on growth, haematological and immunological characteristics in weanling pigs  

Microsoft Academic Search

The study was conducted to determine the effects of iron glycine chelate (Fe-Gly) on growth, haematological and immunological characteristics in weanling pigs. One hundred and eighty pigs (initial weight of 7.81±0.72kg) were allotted to six treatments based on live weight and litter origin. Treatments consisted of: (1) control (no Fe supplementation); (2) 30mgFe\\/kg diet from Fe-Gly; (3) 60mgFe\\/kg diet from

J. Feng; W. Q. Ma; Z. R. Xu; Y. Z. Wang; J. X. Liu

2007-01-01

107

WELFARE AND HAEMATOLOGICAL INDICES OF WEANER RABBITS AS AFFECTED BY STOCKING DENSITY  

Microsoft Academic Search

Forty two weaner rabbits of mixed breeds and sexes with an average initial weight of 956.7±45.6 g were used to evaluate the effect of stocking densit y on welfare and haematological characteristics. Th e rabbits were allotted to four stocking densities of 10, 14.3, 20 and 25 rabbits\\/m 2. This corresponded to 2, 3, 4 and 5 rabbits per cage

108

CLINICAL AND HAEMATOLOGICAL STUDIES ON EXPERIMENTALLY INDUCED SELENOSIS IN CROSSBRED COW CALVES  

Microsoft Academic Search

The effect of sodium selenite induced subacute and chronic toxicity on clinical and haematological parameters was studied in cross-bred cow calves. Subacute and chronic selenosis was induced by oral administration of sodium selenite at dose rate of 2.5 mg\\/kg for 21 days and 0.25 mg\\/kg for 16 weeks, respectively. Toxic manifestations in subacute selenium toxicity included anorexia, salivation, redness of

R. Kaur; S. Rampal; H. S. Sandhu

109

The Efficacy of Nardostachys Jatamansi Against The Radiation Induced Haematological Damage In Rats  

PubMed Central

Introduction: Radiation is increasingly being used for medical purposes and it is an established weapon in the diagnosis and the therapy of cancer. An exposure to 1-2 Gys causes the NVD (Nausea, vomiting, diarrhoea) syndrome, whereas an exposure to 2-6 Gys causes the haematopoietic syndrome. The aim of the present study was to investigate the protective effect of the Nardostachys jatamansi root extract (NJE) on the radiation induced haematological damage in rats. Materials and Methods: EBR was performed at the Microtron Centre, Mangalore University, India. Rats were treated with NJE once daily for 15 days before and after the irradiation. After the irradiation, blood was collected for determining the peripheral blood counts (RBC and WBC), haemoglobin, the platelet count and the packed cell volume (PCV) at 6 hours, 12 hours, 24 hours, 48 hours and 5, 10 and 15 days post irradiation. The data was analyzed by one way ANOVA, followed by the Tukey’s test for multiple comparisons. Result: NJE provided protection against the radiation induced haematological disorders. The rats treated with NJE exhibited a time dependent significant elevation in all the haematological parameters which were studied and its modulation upto the near normal level was recorded. Conclusion: From this study, we concluded that, NJE provides protection by modulating the radiation induced damage on the haematopoietic system.

Gowda, Damodara K M; Shetty, Lathika; A P, Krishna; Kumari, Suchetha N; Sanjeev, Ganesh; P, Naveen

2013-01-01

110

The efficacy of nardostachys jatamansi against the radiation induced haematological damage in rats.  

PubMed

Introduction: Radiation is increasingly being used for medical purposes and it is an established weapon in the diagnosis and the therapy of cancer. An exposure to 1-2 Gys causes the NVD (Nausea, vomiting, diarrhoea) syndrome, whereas an exposure to 2-6 Gys causes the haematopoietic syndrome. The aim of the present study was to investigate the protective effect of the Nardostachys jatamansi root extract (NJE) on the radiation induced haematological damage in rats. Materials and Methods: EBR was performed at the Microtron Centre, Mangalore University, India. Rats were treated with NJE once daily for 15 days before and after the irradiation. After the irradiation, blood was collected for determining the peripheral blood counts (RBC and WBC), haemoglobin, the platelet count and the packed cell volume (PCV) at 6 hours, 12 hours, 24 hours, 48 hours and 5, 10 and 15 days post irradiation. The data was analyzed by one way ANOVA, followed by the Tukey's test for multiple comparisons. Result: NJE provided protection against the radiation induced haematological disorders. The rats treated with NJE exhibited a time dependent significant elevation in all the haematological parameters which were studied and its modulation upto the near normal level was recorded. Conclusion: From this study, we concluded that, NJE provides protection by modulating the radiation induced damage on the haematopoietic system. PMID:23905085

Gowda, Damodara K M; Shetty, Lathika; A P, Krishna; Kumari, Suchetha N; Sanjeev, Ganesh; P, Naveen

2013-06-01

111

C-reactive protein: associations with haematological variables, cardiovascular risk factors and prevalent cardiovascular disease.  

PubMed

C-reactive protein (CRP) has been proposed as a risk factor for cardiovascular disease; however, this association is confounded by mutual relationships with both classical and haematological cardiovascular risk factors. We, therefore, measured CRP with a high-sensitivity assay in stored plasma samples from 414 men and 515 women in the north Glasgow MONICA (MONItoring trends in CArdiovascular diseases) survey, to study its correlation with haematological variables, classical risk factors and prevalent cardiovascular disease. CRP correlated with age, oral contraceptive use, menopause and most classical cardiovascular risk factors (except blood pressure). CRP also correlated with plasma levels of the pro-inflammatory cytokine interleukin 6, and haematocrit, viscosity, red cell aggregation, white cell count, and coagulation factors [fibrinogen, factor (F) VII in women, FVIII, FIX] and inhibitors (antithrombin and protein C in women; protein S) but not coagulation activation markers. CRP was significantly associated with prevalent cardiovascular disease in both men (P = 0.03) and women (P = 0.009), however, the association became non-significant after adjustment for firstly classical risk factors, then fibrinogen. We conclude that correlations with classical and haematological risk factors account for a substantial component of the association of CRP with prevalent cardiovascular disease, but there is evidence of a residual, independent effect among women. PMID:12823355

Woodward, Mark; Rumley, Ann; Lowe, Gordon D O; Tunstall-Pedoe, Hugh

2003-07-01

112

Guidelines on the provision of facilities for the care of adult patients with haematological malignancies (including leukaemia and lymphoma and severe bone marrow failure). British Committee for Standards in Haematology Clinical Haematology Task Force.  

PubMed

This report defines four levels of care required for the management of adult patients with haematological malignancies and marrow failure (acute and chronic leukaemias, lymphomas, myelodysplastic and myeloproliferative disorders, myeloma and severe aplastic anaemia). The higher levels of care require increasing specialist expertise, staffing and resources. Staffing includes both the medical, nursing and scientific/laboratory professions and other support staff. Resources include ward provision, bed numbers, equipment, laboratory and radiotherapy facilities, pharmacy, support services and research. Blood transfusion services and their organisation are discussed separately. The guidelines indicate to providers and purchasers the issues to be considered in placing contracts for the care of these patients. A glossary of terms is provided for purchasers. PMID:7621625

1995-03-01

113

Influence of trypanocidal therapy on the haematology of vervet monkeys experimentally infected with Trypanosoma brucei rhodesiense.  

PubMed

The aim of this study was to characterise the sequential haematological changes in vervet monkeys infected with Trypanosoma brucei rhodesiense and subsequently treated with sub-curative diminazene aceturate (DA) and curative melarsoprol (MelB) trypanocidal drugs. Fourteen vervet monkeys, on a serial timed-kill pathogenesis study, were infected intravenously with 10(4) trypanosomes of a stabilate T. b. rhodesiense KETRI 2537. They were treated with DA at 28 days post infection (dpi) and with MelB following relapse of infection at 140 dpi. Blood samples were obtained from the monkeys weekly, and haematology conducted using a haematological analyser. All the monkeys developed a disease associated with macrocytic hypochromic anaemia characterised by a reduction in erythrocytes (RBC), haemoglobin (HB), haematocrit (HCT), mean cell volume (MCV), platelet count (PLT), and an increase in the red cell distribution width (RDW) and mean platelet volume (MPV). The clinical disease was characteristic of human African trypanosomiasis (HAT) with a pre-patent period of 3 days. Treatment with DA cleared trypanosomes from both the blood and cerebrospinal fluid (CSF). The parasites relapsed first in the CSF and later in the blood. This treatment normalised the RBC, HCT, HB, PLT, MCV, and MPV achieving the pre-infection values within two weeks while RDW took up to 6 weeks to attain pre-infection levels after treatment. Most of the parameters were later characterised by fluctuations, and declined at one to two weeks before relapse of trypanosomes in the haemolymphatic circulation. Following MelB treatment at 140 dpi, most values recovered within two weeks and stabilised at pre-infection levels, during the 223 days post treatment monitoring period. It is concluded that DA and MelB treatments cause similar normalising changes in the haematological profiles of monkeys infected with T. b. rhodesiense, indicating the efficacy of the drugs. The infection related changes in haematology parameters, further characterise the vervet monkey as an optimal induced animal model of HAT. Serial monitoring of these parameters can be used as an adjunct in the diagnosis and prognosis of the disease outcome in the vervet monkey model. PMID:21420376

Ngotho, Maina; Kagira, John M; Kariuki, Christopher; Maina, Naomi; Thuita, John K; Mwangangi, David M; Farah, Idle O; Hau, Jann

2011-07-01

114

Stability of haematological parameters and its relevance on the athlete's biological passport model.  

PubMed

The stability of haematological parameters is crucial to guarantee accurate and reliable data for implementing and interpreting the athlete's biological passport (ABP). In this model, the values of haemoglobin, reticulocytes and out-of-doping period (OFF)-score (Hb-60?Ret) are used to monitor the possible variations of those parameters, and also to compare the thresholds developed by the statistical model for the single athlete on the basis of its personal values and the variance of parameters in the modal group. Nevertheless, a critical review of the current scientific literature dealing with the stability of the haematological parameters included in the ABP programme, and which are used for evaluating the probability of anomalies in the athlete's profile, is currently lacking. In addition, we collected information from published studies, in order to supply a useful, practical and updated review to sports physicians and haematologists. There are some parameters that are highly stable, such as haemoglobin and erythrocytes (red blood cells [RBCs]), whereas others, (e.g. reticulocytes, mean RBC volume and haematocrit) appear less stable. Regardless of the methodology, the stability of haematological parameters is improved by sample refrigeration. The stability of all parameters is highly affected from high storage temperatures, whereas the stability of RBCs and haematocrit is affected by initial freezing followed by refrigeration. Transport and rotation of tubes do not substantially influence any haematological parameter except for reticulocytes. In all the studies we reviewed that used Sysmex instrumentation, which is recommended for ABP measurements, stability was shown for 72 hours at 4 ° C for haemoglobin, RBCs and mean curpuscular haemoglobin concentration (MCHC); up to 48 hours for reticulocytes; and up to 24 hours for haematocrit. In one study, Sysmex instrumentation shows stability extended up to 72 hours at 4 ° C for all the parameters. There are significant differences among methods and instruments: Siemens Advia shows lower stability than Sysmex as regards to reticulocytes. However, the limit of 36 hours from blood collection to analysis as recommended by ABP scientists is reasonable to guarantee analytical quality, when samples are transported at 4 ° C and are accompanied by a certified steadiness of this temperature. There are some parameters that are highly stable, such as haemoglobin and RBCs; whereas others, such as reticulocytes, mean cell volume and haematocrit are more unstable. The stability of haematological parameters might be improved independently from the analytical methodology, by refrigeration of the specimens. PMID:22060177

Lombardi, Giovanni; Lanteri, Patrizia; Colombini, Alessandra; Lippi, Giuseppe; Banfi, Giuseppe

2011-12-01

115

High-dose carboplatin, etoposide and melphalan (CEM) with peripheral blood progenitor cell support as late intensification for high-risk cancer: non-haematological, haematological toxicities and role of growth factor administration.  

PubMed Central

The present report describes the non-haematological toxicity and the influence of growth factor administration on haematological toxicity and haematopoietic recovery observed after high-dose carboplatin (1200 mg m(-2)), etoposide (900 mg m(-2)) and melphalan (100 mg m(-2)) (CEM) followed by peripheral blood progenitor cell transplantation (PBPCT) in 40 patients with high-risk cancer during their first-line treatment. PBPCs were collected during the previous outpatient induction chemotherapy programme by leukaphereses. CEM administration with PBPCT was associated with low non-haematological toxicity and the only significant toxicity consisted of a reversible grade III/IV increase in liver enzymes in 32% of the patients. Haematopoietic recovery was very fast in all patients and the administration of granulocyte colony-stimulating factor (G-CSF) plus erythropoietin (EPO) or granulocyte-macrophage colony-stimulating factor (GM-CSF) plus EPO after PBPCT significantly reduced haematological toxicity, abrogated antibiotic administration during neutropenia and significantly reduced hospital stay and patient's hospital charge compared with patients treated with PBPCT only. None of the patients died early of CEM plus PBPCT-related complications. Low non-haematological toxicity and accelerated haematopoietic recovery renders CEM with PBPC/growth factor support an acceptable therapeutic approach in an adjuvant or neoadjuvant setting.

Benedetti Panici, P.; Pierelli, L.; Scambia, G.; Foddai, M. L.; Salerno, M. G.; Menichella, G.; Vittori, M.; Maneschi, F.; Caracussi, U.; Serafini, R.; Leone, G.; Mancuso, S.

1997-01-01

116

Incidence and outcome of critical illness amongst hospitalised patients with haematological malignancy: a prospective observational study of ward and intensive care unit based care  

Microsoft Academic Search

Summary To determine the incidence and outcome of critical illness amongst the total population of hospital patients with haematological malignancy (including patients treated on the ward as well as those admitted to the intensive care unit), consecutive patients with haematological malign- ancy were prospectively studied. One hundred and one of the 1437 haemato-oncology admis- sions (7%) in 2001 were complicated

A. C. Gordon; H. E. Oakervee; B. Kaya; J. M. Thomas; M. J. Barnett; A. Z. S. Rohatiner; T. A. Lister; J. D. Cavenagh; C. J. Hinds

2005-01-01

117

Haematological parameters in stray dogs seropositive and seronegative to Ehrlichia canis in North Trinidad.  

PubMed

In view of the fact that stray dogs are a reservoir for many diseases, this study was undertaken to determine the prevalence of Ehrlichia canis in stray dogs in North Trinidad and to evaluate the diagnostic implications of haematological alterations associated with seropositivity. Overall, 41 (44.6%) of 92 stray dogs were seropositive to E. canis by the indirect immunofluorescent antibody test. Dogs, one year of age and older (59.7%) were more likely to be seropositive than dogs less than one year old (13.3%) (p<0.001). No significant differences in seropositivity between females and males were found. The odds ratios showed that seropositive dogs were 3.34 (CI 95%; 1.33-8.59) and 5.17 (CI 95%; 0.19-1.26) times more likely to have low platelet counts and elevated total serum protein concentrations (p=0.014 and p<0.001, respectively) than seronegative dogs. Lower mean platelet counts and a higher mean total protein concentration were associated with seropositivity (p<0.01). Mean eosinophil and segmented neutrophil counts were elevated in dogs that tested negative for E. canis antibodies (p=0.002 and p<0.005, respectively). Other haematological parameters were not different between the 2 groups. The high percentage of stray dogs infected with E. canis should alert veterinarians to the potential risk of transmission of the disease. A comprehensive study possibly using molecular methods such as nested PCR should be undertaken to determine how co-infection with other pathogens may alter haematological profiles. In general, control of ticks and stray dog populations may help to control the spread of tick-borne diseases. PMID:22658916

Asgarali, Zinora; Pargass, Indira; Adam, Judy; Mutani, Alexander; Ezeokoli, Chukwudozie

2012-09-01

118

Haematological and biochemical alterations caused by epidural and intramuscular administration of xylazine hydrochloride in dromedary camels (Camelus dromedarius).  

PubMed

This study was conducted in 16 healthy immature dromedary camels weighing 120-150 kg to evaluate and compare the effects of epidural and intramuscular injections of xylazine administered at 0.1 mg/kg and 0.2 mg/kg. Haematological parameters included haemoglobin, packed cell volume, total erythrocyte count and total leukocyte count. Biochemical parameters included alkaline phosphates, alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen, creatinine and glucose. Parameters were measured at different intervals before (baseline) and after the administration of drugs. Our study showed that the effect of xylazine on haematological and biochemical parameters is dose-dependant and is also related to the route of administration. The low dose of xylazine administered using both intramuscular and epidural methods showed minimal effects, whereas high doses of the drug, especially when injected intramuscularly, caused greater changes in haematological and biochemical parameters. PMID:23038078

Azari, Omid; Molaei, Mohammad Mahdi; Emadi, Ladan; Sakhaee, Ehsanollah; Sharifi, Hamid; Mehdizadeh, Sara

2012-01-01

119

Therapy-related classical Hodgkin lymphoma after a primary haematological malignancy: a report on 13 cases.  

PubMed

The risk of developing Hodgkin lymphoma (HL) is increased in immunodeficiencies or during the treatment of some autoimmune diseases. The development of new therapeutic agents has highlighted the risk of unusual lymphoid proliferations, particularly classical HL (cHL). We report the clinicopathological findings of 13 cHL arising in patients treated for a primary haematological malignancy. Eight patients had received an immunomodulator, protein tyrosine-kinase inhibitor or monoclonal antibody, which may have contributed to the cHL development. Most patients had disseminated disease with poor prognostic factors at cHL diagnosis. Despite the initial presentation, good outcomes were achieved with standard cHL chemotherapy. PMID:22734472

Cheminant, Morgane; Galicier, Lionel; Brière, Josette; Boutboul, David; Micléa, Jean-Michel; Venon, Marie-Dominique; Robin, Marie; Thieblemont, Catherine; Brice, Pauline

2012-09-01

120

Haematological and Biochemical Reference Values for Healthy Adults in the Middle Belt of Ghana  

PubMed Central

Background Reference values are very important in clinical management of patients, screening participants for enrolment into clinical trials and for monitoring the onset of adverse events during these trials. The aim of this was to establish gender-specific haematological and biochemical reference values for healthy adults in the central part of Ghana. Methods A total of 691 adults between 18 and 59 years resident in the Kintampo North Municipality and South District in the central part of Ghana were randomly selected using the Kintampo Health and Demographic Surveillance System and enrolled in this cross-sectional survey. Out of these, 625 adults made up of 316 males and 309 females were assessed by a clinician to be healthy. Median values and nonparametric 95% reference values for 16 haematology and 22 biochemistry parameters were determined for this population based on the Clinical Laboratory and Standards Institute guidelines. Values established in this study were compared with the Caucasian values being used currently by our laboratory as reference values and also with data from other African and western countries. Results Reference values established include: haemoglobin 113–164 g/L for males and 88–144 g/L for females; total white blood cell count 3.4–9.2×109/L; platelet count 88–352×109/L for males and 89–403×109/L for females; alanine aminotransferase 8–54 U/L for males and 6–51 U/L for females; creatinine 56–119 µmol/L for males and 53–106 µmol/L for females. Using the haematological reference values based on the package inserts would have screened out up to 53% of potential trial participants and up to 25% of the population using the biochemical parameters. Conclusion We have established a panel of locally relevant reference parameters for commonly used haematological and biochemical tests. This is important as it will help in the interpretation of laboratory results both for clinical management of patients and safety monitoring during a trial.

Dosoo, David K.; Kayan, Kingsley; Adu-Gyasi, Dennis; Kwara, Evans; Ocran, Josephine; Osei-Kwakye, Kingsley; Mahama, Emmanuel; Amenga-Etego, Stephen; Bilson, Philip; Asante, Kwaku P.; Koram, Kwadwo A.; Owusu-Agyei, Seth

2012-01-01

121

Focus on invasive mucormycosis in paediatric haematology oncology patients: a series of 11 cases.  

PubMed

Mucormycosis has emerged as an increasingly important infection in oncology centres with high mortality, especially in severely immunocompromised patients. We carried out a retrospective study of 11 children with mucormycosis treated in seven French oncology-haematology paediatric wards during the period from 1991 to 2011. Lichtheimia corymbifera and Mucor spp. were the predominant pathogens. Treatment regimens included antifungal therapy, reversal of underlying predisposing risk factors and surgical debridement. Although mucormycosis is associated with high mortality, this infection could be cured in eight of our cases of severely immunocompromised paediatric cancer patients. PMID:23020159

Phulpin-Weibel, Aurélie; Rivier, Alexandre; Leblanc, Thierry; Bertrand, Yves; Chastagner, Pascal

2013-05-01

122

Effect in vivo of atrazine on haematology and O2 consumption in fish, Tilapia mossambica.  

PubMed

Studies were conducted on haematological constituents such as Red blood cells (RBC), White blood cells (WBC), Haemoglobin (Hb), Packed cell volume (PCV), Mean cell volume (MCV), Mean corpuscular haemoglobin (MCH), Mean corpuscular haemoglobin concentration (MCHC), Blood volume (BV), Blood water content (BWC) and Whole animal oxygen consumption (WAOC) in the fish exposed to sublethal concentration of atrazine. Significant changes were seen in the constituents of the blood and O2 consumption of fish suggesting the existence of respiratory distress in the fish as a consequence of atrazine toxicity. PMID:1863269

Prasad, T A; Srinivas, T; Rafi, G M; Reddy, D C

1991-01-01

123

Fifteenth biannual report of the Cochrane Haematological Malignancies Group--focus on non-Hodgkin's lymphoma.  

PubMed

This fifteenth biannual report of the Cochrane Haematological Malignancies Group (CHMG) highlights recently published randomized controlled trials (RCTs) in the field of hemato-oncology, covering the publication period from October 2011 to May 2012. Implications for clinical practice and methodological aspects are the main principles for selecting trials for this report. Studies were identified by electronic search of MEDLINE using a broad search filter that covers all topics in hemato-oncology combined with a highly sensitive search filter for randomized trials (Cochrane Handbook for Systematic Reviews of Interventions). PMID:23852951

Rancea, Michaela; Will, Andrea; Borchmann, Peter; Monsef, Ina; Engert, Andreas; Skoetz, Nicole

2013-08-01

124

The role of primary antifungal prophylaxis in patients with haematological malignancies.  

PubMed

Invasive fungal infections (IFIs) represent important complications in patients with haematological malignancies. Chemoprevention of IFIs may play an important role in this setting, but in the past decades the majority of antifungal drugs utilized demonstrated poor efficacy, particularly in the prevention of invasive aspergillosis. The new triazoles are very useful antifungal drugs, more suitable for prophylaxis of IFIs than amphotericin B and echinocandins. In this review, the main clinical data about antifungal prophylaxis with fluconazole, itraconazole, voriconazole and posaconazole are analysed. At present, posaconazole appears to be the most efficacious azole in antifungal prophylaxis, particularly in patients with acute myeloid leukaemia. PMID:24372659

Pagano, L; Caira, M

2014-06-01

125

Haematological and iron-related parameters in male and female athletes according to different metabolic energy demands  

Microsoft Academic Search

We investigated the iron-related haematological parameters in both male and female athletes participating in different sporting\\u000a disciplines necessitating different metabolic energy demands. A total of 873 athletes (514 males, mean age: 22.08 ± 4.95 years\\u000a and 359 females, mean age: 21.38 ± 3.88 years) were divided according to gender and to the predominant energy system required\\u000a for participation in sport (aerobic, anaerobic or mixed) and haematological

Radoje Milic; Jelena Martinovic; Milivoj Dopsaj; Violeta Dopsaj

2011-01-01

126

Establishment of haematological and immunological reference values for healthy Tanzanian children in Kilimanjaro Region  

PubMed Central

Summary Objective To determine the normal haematological and immunological reference intervals for healthy Tanzanian children. Methods We analysed data from 655 HIV-seronegative, healthy children from 1 month to 18 years of age from the Kilimanjaro Region of Tanzania for this cross-sectional study. Median and 95% reference ranges were determined for haematological and immunological parameters and analysed by age cohorts, and by gender for adolescents. Results Median haemoglobin (Hb) and haematocrit (Hct) for all age groups were higher than established East African reference intervals. Compared to U.S. intervals, reference ranges encompassed lower values for Hb, Hct, mean corpuscular volume, and platelets. Applying the U.S. National Institute of Health Division of AIDS (DAIDS) adverse event grading criteria commonly used in clinical trials to the reference range participants, 128 (21%) of 619 children would be classified as having an adverse event related to Hb level. CD4-positive T-lymphocyte absolute counts declined significantly with increasing age (P < 0.0001). For those aged under five years, CD4-positive T-lymphocyte percentages are lower than established developed country medians. Conclusions Country-specific reference ranges are needed for defining normal laboratory parameters among children in Africa. Knowledge of appropriate reference intervals is critical not only for providing optimal clinical care, but also for enrolling children in medical research. Knowledge of normal CD4-positive T-lymphocyte parameters in this population is especially important for guiding the practice of HIV medicine in Tanzania.

Buchanan, Ann M.; Muro, Florida J.; Gratz, Jean; Crump, John A.; Musyoka, Augustine M.; Sichangi, Moses W.; Morrissey, Anne B.; M'rimberia, Jane K.; Njau, Boniface N.; Msuya, Levina J.; Bartlett, John A.; Cunningham, Coleen K.

2010-01-01

127

Guideline on the prevention of secondary central nervous system lymphoma: British Committee for Standards in Haematology.  

PubMed

The guideline group was selected to be representative of UK-based medical experts. Ovid MEDLINE, EMBASE and NCBI Pubmed were searched systematically for publications in English from 1980 to 2012 using the MeSH subheading 'lymphoma, CNS', 'lymphoma, central nervous system', 'lymphoma, high grade', 'lymphoma, Burkitt's', 'lymphoma, lymphoblastic' and 'lymphoma, diffuse large B cell' as keywords, as well as all subheadings. The writing group produced the draft guideline, which was subsequently revised by consensus by members of the Haemato-oncology Task Force of the British Committee for Standards in Haematology (BCSH). The guideline was then reviewed by a sounding board of ~50 UK haematologists, the BCSH and the British Society for Haematology (BSH) Committee and comments incorporated where appropriate. The 'GRADE' system was used to quote levels and grades of evidence, details of which can be found in Appendix I. The objective of this guideline is to provide healthcare professionals with clear guidance on the optimal prevention of secondary central nervous system (CNS) lymphoma. The guidance may not be appropriate to patients of all lymphoma sub-types and in all cases individual patient circumstances may dictate an alternative approach. Acronyms are defined at time of first use. PMID:24033102

McMillan, Andrew; Ardeshna, Kirit M; Cwynarski, Kate; Lyttelton, Matthew; McKay, Pam; Montoto, Silvia

2013-10-01

128

Alfalfa dodder (Cuscuta campestris) toxicity in horses: clinical, haematological and serum biochemical findings.  

PubMed

The objective of this observational study is to describe clinical, haematological and serum biochemical findings of horses affected with alfalfa dodder (Cuscuta campestris) toxicity. Twenty horses naturally exposed to alfalfa dodder toxicity were examined and information was collected on history and clinical signs. Physical examination was done on horses in the premises (n=20), and venous blood samples of 12 horses were submitted for haematology and serum biochemical examination for each horse. Abnormal clinical signs started around 36 hours after horses were fed the contaminated alfalfa. Abnormal signs were seen in 11 horses and those included diarrhoea (n=8), decreased appetite (n=7), neurological signs (n=4) and abdominal pain (n=1). Some horses had multiple clinical signs of the above. The results of complete blood cell count revealed leukocytopenia, neutropenia and thrombocytopenia. Serum biochemical analysis revealed decreased ALP, AST and CPK levels and increased direct bilirubin level. The used alfalfa was stopped immediately and a different alfalfa from a new container that did not contain any weeds was fed. Horses on the premises were observed closely, and the abnormal clinical signs resolved within three days. No treatment was implemented. Knowledge about toxicity of horses by Cuscuta species is scarce in the English veterinary literature and very limited. PMID:23800626

Abutarbush, S M

2013-07-27

129

Biochemical and haematologic effects of intake of Macrotermes nigeriensis fortified functional diet.  

PubMed

Twenty-four male albino rats, 4 weeks old were randomly distributed into 4 groups (A-D) and fed growers' mash as the control diet and 25, 50 and 75% oven-dried, ground Macrotermes nigeriensis fortified diets respectively for a total period of 28 days. The effects of the diets on hepatic, renal and haematologic function indices of albino rats were studied. The animals fed the fortified diets had non-significantly (p > 0.05) lesser body weight gains than the control animals. The fortified diets elicited dose-dependent increases in the levels of blood total protein, albumin, packed cell volume, hemoglobin and red blood cell counts as well as on the calculated red cell indices. The fortified diets did not significantly (p > 0.05) alter the activities of plasma alanine and aspartate aminotransferases as well as the concentrations of total bilirubin, urea and creatinine. The results indicate that the fortified diets do not have detrimental hepatic, renal or haematologic effects but rather may be recommended for fortification of human and animal food, especially in weaning diets of growing children and nursing mothers to combat food insecurity and malnutrition. PMID:24783815

Igwe, C U; Ojiako, A O; Okwara, J E; Emejulu, A A; Nwaoguikpe, R N

2014-01-15

130

Monitoring environmental Aspergillus spp. contamination and meteorological factors in a haematological unit.  

PubMed

The opportunistic pathogens belonging to the Aspergillus genus are present in almost all seasons of the year, and their concentration is related to meteorological conditions. The high density of Aspergillus spp. conidia in a haematological hospital ward may be a significant risk factor for developing invasive fungal diseases in immunocompromised patients. Aim of the present study was to evaluate the variability of airborne Aspergillus spp. conidia contamination in a Haematological Unit (HU) within a period of 16 months in relation with some meteorological parameters. An environmental Aspergillus surveillance was conducted in the HU in four rooms and their bathrooms, in the corridor and in three external sites using an agar impact sampler. During each sampling, temperature and relative humidity at each site were recorded and current wind speed and rainfall events were taken from the official weather service. Aspergillus spp. conidia concentration differed significantly across the sampling sites. Internal Aspergillus spp. loads were significantly dependent on temperature, internal relative humidity and rain. External conidia concentrations were significantly influenced by outdoor temperature and relative humidity. A suitable indicator was introduced to evaluate the seasonal distribution of Aspergillus spp. conidia in the sampling sites, and a significant dependence on this indicator was observed inside the HU. Seventeen different fungal species belonging to the Aspergillus genus were detected during the sampling period. Aspergillus fumigatus was the most frequently isolated species and its distribution depended significantly on the seasonal indicator both inside and outside the hospital ward. PMID:24158616

Cavallo, M; Andreoni, S; Martinotti, M G; Rinaldi, M; Fracchia, L

2013-12-01

131

Serial haematology results in transfused and non-transfused dogs naturally infected with Babesia rossi.  

PubMed

This prospective longitudinal study investigated the progression of haematological changes in 32 transfused and 54 non-transfused dogs naturally infected with Babesia rossi over the 1st 6 days following diagnosis and treatment. The effect of patient age on the results of complete blood counts was determined. Haematology data were analysed at presentation and at 24 hours, 3 days and 6 days after presentation. Dogs were treated with diminazene aceturate at diagnosis and a blood transfusion was given if deemed clinically required. Mildly to moderately regenerative normocytic normochromic anaemia was observed in all dogs throughout the study period. Transfused dogs more often had an inflammatory leukogram at presentation and at 24 hours, than dogs that were not transfused. In dogs with a left shift, a concurrent normal or decreased segmented neutrophil count was found more commonly than neutrophilia. Severe thrombocytopenia that resolved within a week was common. Blood transfusion alleviated the anaemia, but had no significant effect on white blood cell or platelet responses. Blood cell responses were not significantly influenced by age. In conclusion, the red blood cell and white blood cell responses were less than expected in dogs with babesiosis, given the degree of anaemia and inflammation present. The magnitude of thrombocytopenia and rapid return of the platelet count to normal suggested a possible immune-mediated mechanism for the thrombocytopenia. PMID:22332296

Scheepers, E; Leisewitz, A L; Thompson, P N; Christopher, M M

2011-09-01

132

Haematological and biochemical values for grey seal pups (Halichoerus grypus) during early rehabilitation.  

PubMed

Haematological and biochemical data were collected over a period of six years from grey seal (Halichoerus grypus) pups undergoing rehabilitation. Pups bled during the first three days were allocated retrospectively to one of seven clinical groups and statistical analyses were carried out on six of these groups (130 pups). Compared with reference ranges, all the groups had lower mean packed-cell volumes (pcv), red blood cell (rbc) counts haemoglobin and albumin levels, pups with severe trauma had higher total white blood cell (wbc) and neutrophil counts, pups with severe trauma and malnourished pups had higher total bilirubin levels, and pups in all the clinical groups except those with severe trauma had lower sodium levels. There were significant differences (P<0.05) between some clinical groups for mean pcv, rbc counts, all wbc counts except monocytes, haemoglobin, total bilirubin, creatine kinase, amylase and potassium levels. Forty-nine pups were bled more than once during the first 10 days of rehabilitation for haematology, and 11 were bled more than once for biochemistry. There were significant decreases (P<0.05) during this period in pcv, and in the activities of alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, creatine kinase and lactate dehydrogenase, and significant increases in all wbc counts, total protein, globulin, amylase and calcium levels. PMID:17906226

Barnett, J E F; Turner, L; Booth, P A; Hunt, A E

2007-09-29

133

Haematology and serum biochemistry of captive unsedated chital deer (Axis axis) in Australia.  

PubMed

Haematological and serum biochemical values were measured in blood samples collected over a 12-mo period from 37 unsedated chital deer (Axis axis). Stags and hinds, ranging in age from birth to maturity, were sampled. Haemoglobin, total erythrocyte and haematocrit values were low at birth and sex differences were not apparent in neonates and juveniles, but were in adults. Chital stags had higher erythrocyte parameters (P less than 0.001) and lower erythrocyte indices than hinds, and the total leucocyte count was higher in stags (P less than 0.01). Some parameters (erythrocytes, muscle enzymes, glucose, cortisol) decreased over successive serial sampling. The differential leucocyte count of older stags decreased during the initial handling period. The major rutting period in February and March was characterised by changes in the differential leucocyte count, elevations in serum muscle enzymes, and lower serum cortisol levels. Alkaline phosphatase activity in serum reflected the annual antler cycle of chital stags. Serial sampling over many weeks, either weekly or tri-weekly, produced haematological and biochemical changes in successive samples which may have reflected a reduction in stress and excitement associated with restraint. PMID:1920658

Chapple, R S; English, A W; Mulley, R C; Lepherd, E E

1991-07-01

134

Activity of cyclosporins as resistance modifiers in primary cultures of human haematological and solid tumours.  

PubMed Central

The semiautomated fluorimetric microculture cytotoxicity assay (FMCA) was used for evaluation of the ability of cyclosporin A (CsA) and its novel non-immunosuppressive derivative SDZ PSC 833 (PSC) to modify the response to doxorubicin or vincristine in vitro in different haematological and solid human tumour types. Primary cultures of 322 tumour samples were analysed. Both cyclosporins showed resistance-modifying activity in all haematological tumours tested, and in solid tumours activity was observed in ovarian carcinoma and childhood tumours. Little or no effect was found in the remaining tumour types, including breast, renal and adrenal cortical carcinomas and adult sarcomas. In most of the responsive cases the interaction between the modifier and the cytotoxic drug was synergistic. There was a tendency to higher activity in samples from previously treated patients, and an inverse relationship between degree of cytotoxic drug resistance and resistance-modifying activity was noted. No difference in potency between CsA and PSC could be discerned. The results indicate differential in vitro resistance-modifying activity of the cyclosporins depending on tumour type. The results also suggest that treatment with resistance modifiers should be considered also for primary therapy of drug-sensitive tumours. Drug resistance assays such as the FMCA may become useful in preclinical evaluation of resistance modifiers.

Fridborg, H.; Jonsson, B.; Nygren, P.; Csoka, K.; Nilsson, K.; Oberg, G.; Kristensen, J.; Bergh, J.; Tholander, B.; Olsen, L.

1994-01-01

135

Survival following relapse in childhood haematological malignancies diagnosed in 1974-2003 in Yorkshire, UK  

PubMed Central

We examined population-based information on relapsed childhood haematological cancers, investigating factors that might influence both overall survival and survival following relapse among the 1177 children (0–14 years) diagnosed with a haematological malignancy in Yorkshire from 1974 to 2003, of whom 342 (29%) relapsed at least once. Leukaemia patients from more deprived areas were significantly less likely to relapse (odds ratio=0.54, 95% confidence interval 0.32–0.93 for most deprived quintile vs least deprived quintile; Ptrend=0.06), especially those with acute myeloid leukaemia (P=0.04). Neither ethnic group nor distance to the main treatment centre was associated with risk of relapse. Overall, patients who relapsed at least once had 5-year survival rates of 46% (41–51%) compared with 79% (76–81%) of those who did not. Five-year survival rates from the time of first relapse increased from 20% in 1974–1983 to 45% in 1984–2003. Length of first remission was a strong predictor of survival for leukaemia with a 46% reduced risk of death for every additional year of event-free survival. Of children who experienced a relapse, 46% survived at least 5 years, whereas just under half of patients survived 5 years beyond disease recurrence. This provides a baseline for future comparisons and demonstrates that relapsed childhood cancer need not imply a poor outcome.

Feltbower, R G; Kinsey, S E; Richards, M; Shenton, G; Michelagnoli, M P; McKinney, P A

2007-01-01

136

Performance evaluation of the Sysmex XS-1000i automated haematology analyser.  

PubMed

The Sysmex XS-1000i is a compact new, fully automated haematology analyser, designed to generate complete blood counts with five-part leucocyte differential. In our study, a Sysmex XS-1000i instrument was evaluated according to Clinical Laboratory Standards Institute (CLSI) and International Council for Standardization in Haematology (ICSH) guidelines. Precision, carry-over and linearity were determined. Using a total of 700 patient samples, results from the Sysmex XS-1000i were compared with those from a Sysmex XE-2100, an Abbott Cell Dyn 4000 and the manual reference leucocyte differential. Using quality control material, total and within-run imprecision was less than 3% except for platelets. The system demonstrated good linearity over the entire reporting range and no carry-over (<0.5%). The Sysmex XS-1000i showed good correlation with XE-2100, CD-4000 and the manual reference leucocyte differential. Overall flagging sensitivity and specificity were 91% and 48%, respectively. In conclusion, the Sysmex XS-1000i demonstrated good analytical performance, is able to generate a complete blood count with five-part differential on low blood volumes and has considerable back-up capacity. PMID:18616754

Ghys, T; Malfait, R; VAN den Bossche, J

2009-10-01

137

Dielectric, haematological and biochemical studies of detergent toxicity in fish blood.  

PubMed

Blood characteristics in dielectric, haematological and biochemical terms of the fish Cyprinus carpio exposed to a sublethal concentration of sodium alkyl benzene sulphonate were compared with those from untreated control fish; recovery from the test solution was also checked. Trends of change in the majority of chosen parameters of blood with time of fish exposure to anionic detergent were significantly linear. A decline was noted in erythrocyte count, haematocrit, blood haemoglobin concentration, mean corpuscular haemoglobin concentration and mean corpuscular haemoglobin with the exception of mean cellular volume. An uptake of sodium into red blood cells and a rise of intracellular potassium were seen. Practical indicators of the presence of detergent in fish blood were obtained from dielectric beta dispersion measurements. It has been shown that dielectric parameters could be correlated with haematological parameters but not with a biochemical one. Results indicate that the changes in haematocrit induce corresponding variation of the maximum of the dielectric loss factor tan delta. An increase in the electrical conductivity and permittivity of the erythrocytes' interior in exposed fish was associated with a decline of mean corpuscular haemoglobin. PMID:3035591

Bielinska, I

1987-05-01

138

Alterations in cytokines and haematological parameters during the acute and convalescent phases of Plasmodium falciparum and Plasmodium vivax infections.  

PubMed

Haematological and cytokine alterations in malaria are a broad and controversial subject in the literature. However, few studies have simultaneously evaluated various cytokines in a single patient group during the acute and convalescent phases of infection. The aim of this study was to sequentially characterise alterations in haematological patters and circulating plasma cytokine and chemokine levels in patients infected with Plasmodium vivax or Plasmodium falciparum from a Brazilian endemic area during the acute and convalescent phases of infection. During the acute phase, thrombocytopaenia, eosinopaenia, lymphopaenia and an increased number of band cells were observed in the majority of the patients. During the convalescent phase, the haematologic parameters returned to normal. During the acute phase, P. vivax and P. falciparum patients had significantly higher interleukin (IL)-6, IL-8, IL-17, interferon-?, tumour necrosis factor (TNF)-?, macrophage inflammatory protein-1? and granulocyte-colony stimulating factor levels than controls and maintained high levels during the convalescent phase. IL-10 was detected at high concentrations during the acute phase, but returned to normal levels during the convalescent phase. Plasma IL-10 concentration was positively correlated with parasitaemia in P. vivax and P. falciparum-infected patients. The same was true for the TNF-? concentration in P. falciparum-infected patients. Finally, the haematological and cytokine profiles were similar between uncomplicated P. falciparum and P. vivax infections. PMID:24676654

Rodrigues-da-Silva, Rodrigo Nunes; Lima-Junior, Josué da Costa; e Fonseca, Bruna de Paula Fonseca; Antas, Paulo Renato Zuquim; Baldez, Arlete; Storer, Fabio Luiz; Santos, Fátima; Banic, Dalma Maria; de Oliveira-Ferreira, Joseli

2014-04-01

139

ICSH Guideline for worldwide point-of-care testing in haematology with special reference to the complete blood count  

Microsoft Academic Search

SUMMARY These guidelines provide information on how to develop and manage a point-of-care (POCT) service so that reliable haematology results are produced regardless of where the test is performed. Many of the issues addressed here are relevant to POCT within hospitals or health centres; however, the principles are equally applicable to care in the commu- nity and doctors' offices. Other

C. BRIGGS; J. CARTER; S.-H. LEE; L. SANDHAUS; R. SIMON-LOPEZ; J.-L. VIVES CORRONS

2008-01-01

140

Iron, but not folic acid, combined with effective antimalarial therapy promotes haematological recovery in African children after acute falciparum malaria  

Microsoft Academic Search

Whether children with malarial anaemia should receive supplementation with iron or folic acid is uncertain. Therefore, the effects of supplementary treatment with iron or folic acid, given together with chloroquine or pyrimethamine-sulfadoxine (Fansidar®), has been assessed in 600 Gambian children with uncomplicated falciparum malaria. After one month, haematological recovery was significantly better in the group treated with Fansidar® than in

Michaël Boele van Hensbroek; Stephen Morris-Jones; Sarah Meisner; Shabbar Jaffar; Lang Bayo; Raduwan Dackour; Christine Phillips; Brian M. Greenwood

1995-01-01

141

Pre-adaptation, adaptation and de-adaptation to high altitude in humans: cardio-ventilatory and haematological changes  

Microsoft Academic Search

The aim of this study was first to investigate cardio-ventilatory and haematological responses induced by intermittent acclimation and second to study de-adaptation from high altitude observed after descent. To achieve these objectives nine subjects were submitted to intermittent acclimation in a low barometric chamber (8 h daily for 5 days, day 1 at 4500 m, day 5 at 8500 m)

Gustave Savourey-Nathalie; Nathalie Garcia; Yves Besnard; Angélique Guinet; Anne-Marie Hanniquet; Jacques Bittel

1996-01-01

142

Effects of vitamin E, age and sex on performance of Japanese quail. 1. Haematological indices and liver function  

Microsoft Academic Search

1. A trial was conducted to examine the effects of dietary vitamin E content, age and sex on haematological indices and liver enzymes of Japanese quails. A total of 800 1-d-old quail chicks were assigned at random into 4 equal groups and fed on starter and layer diets containing 0, 1, 5 or 10 times the NRC recommended supplements of

M. A. Elaroussi; M. A. Fattah; N. H. Meky; I. E. Ezzat; M. M. Wakwak

2007-01-01

143

Vanadium toxicology – an assessment of general health, haematological aspects and energy response in an Indian catfish Clarias batrachus (Linn)  

Microsoft Academic Search

The pervasive occurrence of vanadium in nature and its use in various industrial processes has increased its inputs in the environment. This has prompted us to elucidate the impact of vanadium on aquatic environ-ment, the primary body for industrial effluent discharge. The energy response of the fish, Clarias batra-chus, its haematological status including haemoglobin (Hb), haematocrit (Ht), leutocrit (Lt), mean

Anindita Chakraborty; Somita Oinam; Ranajit Karmakar; Malay Chatterjee

1998-01-01

144

Haematological measurements as an aid to early diagnosis and prognosis of respiratory viral infections in thoroughbred horses  

Microsoft Academic Search

In late November 1988 large numbers of thoroughbred horses in training in Hong Kong developed a transient pyrexia with, in some cases, the clinical signs of viral respiratory disease. Serial blood samples for haematological examination were taken from 10 of the horses which were stabled in six different blocks. They had developed a high temperature within three days of each

DK Mason; KL Watkins; JT McNie; CM Luk

1990-01-01

145

CHANGES IN HAEMATOLOGICAL INDICES AND PROTEIN CONCENTRATIONS IN TRYPANOSOMA BRUCEI INFECTED RATS TREATED WITH HOMIDIUM CHLORIDE AND DIMINAZENE ACETURATE  

Microsoft Academic Search

Anaemia and immunosuppresion have been shown to be a cardinal feature in African try- panosomosis. In this study, we have evaluated and compared the capacity of two registered veterinary trypanocides Novidium® (homidium chloride) and Berenil® (diminazene acetu- rate) to reduce haematological and biochemical lesions in rats experimentally infected with T. brucei. Packed cell volume (PCV), lymphocyte and eosinophil values in

Sulaiman Faoziyat Adenike; Adeyemi Oluyomi Stephen

2010-01-01

146

Alterations in cytokines and haematological parameters during the acute and convalescent phases of Plasmodium falciparum and Plasmodium vivax infections  

PubMed Central

Haematological and cytokine alterations in malaria are a broad and controversial subject in the literature. However, few studies have simultaneously evaluated various cytokines in a single patient group during the acute and convalescent phases of infection. The aim of this study was to sequentially characterise alterations in haematological patters and circulating plasma cytokine and chemokine levels in patients infected with Plasmodium vivax or Plasmodium falciparum from a Brazilian endemic area during the acute and convalescent phases of infection. During the acute phase, thrombocytopaenia, eosinopaenia, lymphopaenia and an increased number of band cells were observed in the majority of the patients. During the convalescent phase, the haematologic parameters returned to normal. During the acute phase, P. vivax and P. falciparum patients had significantly higher interleukin (IL)-6, IL-8, IL-17, interferon-?, tumour necrosis factor (TNF)-?, macrophage inflammatory protein-1? and granulocyte-colony stimulating factor levels than controls and maintained high levels during the convalescent phase. IL-10 was detected at high concentrations during the acute phase, but returned to normal levels during the convalescent phase. Plasma IL-10 concentration was positively correlated with parasitaemia in P. vivax and P. falciparum-infected patients. The same was true for the TNF-? concentration in P. falciparum-infected patients. Finally, the haematological and cytokine profiles were similar between uncomplicated P. falciparum and P. vivax infections.

Rodrigues-da-Silva, Rodrigo Nunes; Lima-Junior, Josue da Costa; Fonseca, Bruna de Paula Fonseca e; Antas, Paulo Renato Zuquim; Baldez, Arlete; Storer, Fabio Luiz; Santos, Fatima; Banic, Dalma Maria; de Oliveira-Ferreira, Joseli

2014-01-01

147

Quality control of the total lymphocyte count parameter obtained from routine haematology analyzers, and its relevance in HIV management.  

PubMed

Lylmphocyte subsets/CD4 T Helper cell enumeration in HIV care and treatment in resource constrained settings can be difficult to ascertain as a result of the lack of the necessary instrumentation, capacity and infrastructure. However. it is imperative to gain such information for patient monitoring in HIV. The Total Lymphocyte Count (TLC) is useful as a surrogate marker for CD4 count as recommended by the World Health Organisation (WHO) and to calculate CD4% for pacdiatric use. This study therefore sets out to determine and compare the accuracy of the total lymphocyte counts obtained from three haematology analysers designated A. B and C. that are in regular use for routine haemnatological parameters at the main referral hospital in Barbados. West Indies. The TLC of 263 HIV treatment naive individuals attending the HIV Reference Unit in Barbados were enumnerated on the three haematology analysers. The lymphosumn (Sum of lymphocyte subsets: T-helper cell. T-cytotoxic cells. B lymphocytes and Natural killer cells) should be equal to the TLC. and is derived by immunophenotypic analysis on a 4-colour flowcytometer. Machine C had the highest positive correlation between the TLC and the lymphosumn with and R' of 0.9031 compared to machine A with an R values of 0.7119 and Machine B with R(2) values of 0.637. These results show that there can be dramatic inaccuracies when using routine haematology analysers for both routine use. as a surrogate marker of CD4 or for derivation of CD4% in HIV management. It further demonstrates that all haematology analyzers require some form of Quality control. The possible lack of accuracy of the TLC by haematology analysers should be taken into consideration when following the recommendations of the WHO in resource poor settings or using it as a denominator for calculating CD4%. PMID:17209312

Marshall, S; Sippy, N; Broome, H; Abayomi, A

2006-06-01

148

Support vector machine versus logistic regression modeling for prediction of hospital mortality in critically ill patients with haematological malignancies  

PubMed Central

Background Several models for mortality prediction have been constructed for critically ill patients with haematological malignancies in recent years. These models have proven to be equally or more accurate in predicting hospital mortality in patients with haematological malignancies than ICU severity of illness scores such as the APACHE II or SAPS II [1]. The objective of this study is to compare the accuracy of predicting hospital mortality in patients with haematological malignancies admitted to the ICU between models based on multiple logistic regression (MLR) and support vector machine (SVM) based models. Methods 352 patients with haematological malignancies admitted to the ICU between 1997 and 2006 for a life-threatening complication were included. 252 patient records were used for training of the models and 100 were used for validation. In a first model 12 input variables were included for comparison between MLR and SVM. In a second more complex model 17 input variables were used. MLR and SVM analysis were performed independently from each other. Discrimination was evaluated using the area under the receiver operating characteristic (ROC) curves (± SE). Results The area under ROC curve for the MLR and SVM in the validation data set were 0.768 (± 0.04) vs. 0.802 (± 0.04) in the first model (p = 0.19) and 0.781 (± 0.05) vs. 0.808 (± 0.04) in the second more complex model (p = 0.44). SVM needed only 4 variables to make its prediction in both models, whereas MLR needed 7 and 8 variables in the first and second model respectively. Conclusion The discriminative power of both the MLR and SVM models was good. No statistically significant differences were found in discriminative power between MLR and SVM for prediction of hospital mortality in critically ill patients with haematological malignancies.

Verplancke, T; Van Looy, S; Benoit, D; Vansteelandt, S; Depuydt, P; De Turck, F; Decruyenaere, J

2008-01-01

149

[Therapeutic observance in haematology: the particular case of chronic myeloid leukemia].  

PubMed

Therapeutic adhesion is essential because it modulates the efficiency of treatment, the occurrence of side effects and the socioeconomic costs possibly associated with it. In haematology, in the particular case of Chronic Myeloid Leukemia, the "ADAGIO" study performed by Noens and co-workers demonstrated that therapeutic observance is lower than the practitioners, the patients and the family members believe it is. This lack of adhesion can engender a decrease of the efficiency of treatment, in particular by imatinib. Factors such as the chronicity of the disease and the length of treatment modify therapeutic adhesion over time. Simple methods, addressing both the patient and the doctor should be developed to improve this therapeutic adhesion. PMID:20684429

Roufosse, R; Beguin, Y

2010-01-01

150

Effects of two diets on the haematology, plasma chemistry and intestinal flora of budgerigars (Melopsittacus undulatus).  

PubMed

Two groups of 22 budgerigars (Melopsittacus undulatus) were housed for 12 months under identical conditions. One group was fed a commercial seed mixture plus carrots and a mineral supplement, and the other group was fed a commercially formulated diet plus carrots. Samples of blood and faeces were collected initially and after three, six, nine and 12 months. There were no significant differences between the haematological values of the two groups. The group fed the seed mixture had significantly higher concentrations of glucose, albumin, triglycerides and uric acid, and higher activity of aspartate aminotransferase, but the values were within the published reference ranges for normal birds. There were no significant differences between the faecal samples from the two groups, except that the fungus Macrorhabdus ornithogaster was identified in 48.3 per cent of the samples from the group fed the commercially formulated diet but from only 3.4 per cent of the samples from the group fed the seed mixture. PMID:17028249

Fischer, I; Christen, C; Lutz, H; Gerlach, H; Hässig, M; Hatt, J-M

2006-10-01

151

Haematological alterations in Rattus norvegicus (Wistar) experimentally infected with Echinostoma paraensei (Trematoda: Echinostomatidae).  

PubMed

Laboratory rats (Rattus norvegicus) were infected with Echinostoma paraensei (Trematoda: Echinostomatidae). The rodents received 150 metacercariae each and blood samples were collected weekly until the fifth week of infection. The blood samples were analyzed for determination of haematocrit, total red blood cells with their dimensions, haemoglobin and haematimetric index (mean corpuscular volume, MCV; mean corpuscular haemoglobin, MCH; and mean corpuscular haemoglobin concentration, MCHC) and platelets. Red blood cells, haematocrit and haemoglobin in the first week had significantly lower levels than those of uninfected (control) rats, suggesting the development of normocytic and normocromic anaemia with anisocytic alteration. The number of eosinophils did not increase significantly among the groups. We concluded that E. paraensei produces haematological alterations in R. norvegicus, causing regenerative anaemia. This system can therefore be a useful model to study the direct and indirect effects of gastrointestinal infections. PMID:22579577

Garcia, J S; Pinheiro, J; Hooper, C S; Simões, R O; Ferraz, J S; Maldonado, A

2012-07-01

152

Sixteenth Biannual Report of the Cochrane Haematological Malignancies Group: Focus on Non-Hodgkin's Lymphoma.  

PubMed

This sixteenth biannual report of the Cochrane Haematological Malignancies Group highlights recently published randomized controlled trials (RCTs) in the field of hemato-oncology, with special focus on non-Hodgkin's lymphoma. The report covers the publication period June 2012 to July 2013. Trials are selected regarding their methodology and implication for clinical practice. Studies were identified by electronic search of MEDLINE using a broad search filter that covers all topics in hemato-oncology combined with a highly sensitive search filter for randomized trials (Cochrane Handbook for Systematic Reviews of Interventions). Four RCTs are presented in detail, followed by two further RCTs of high importance in a short version. The report is finalized with an overview of new and updated Cochrane Reviews. PMID:25031306

Rancea, Michaela; Will, Andrea; Borchmann, Peter; Monsef, Ina; Engert, Andreas; Skoetz, Nicole

2014-08-01

153

Changes of the immunological and haematological parameters in rabbits after bendiocarbamate application.  

PubMed

The effect of bendiocarbamate application (5 mg/kg b.w.) on the haematological and immunological parameters in rabbits was evaluated. Total leukocyte cell count, erythrocyte cell count, differential cell count were determined during the period of three months of bendiocarbamate application and compared with those in healthy animals. The immunotoxic effect was evaluated by the test of ingestion ability of phagocytes (phagocytic activity and index of phagocytic activity) and proliferation activity of lymphocytes after mitogen stimulation. The significant decrease of total leukocytes, lymphocytosis and neutropaenia were found after bendiocarbamate application. The functional activities of phagocytes (expressed as phagocytic activity) and lymphocytes (proliferative activity) were significantly suppressed in rabbits treated with bendiocarbamate compared with those in control groups and values before the experiment. PMID:22540647

Mojzisova, Jana; Massányi, Peter; Danko, Jan; Trbolova, Alexandra; Petrovova, Eva; Mazensky, David; Vdoviakova, Katarina; Luptakova, Lenka; Torma, Norbert

2012-01-01

154

Haematological and biochemical measurements in a population of wild Eurasian badgers (Meles meles).  

PubMed

Blood samples were collected from a high density population of wild badgers in Woodchester Park, Gloucestershire, England, where animals were routinely captured and examined as part of a long-term ecological study, and a selection of haematological and biochemical variables were measured. The badger cubs had lower red blood cell counts and haemoglobin concentrations than the adults, consistent with physiological anaemia, and lower serum protein concentrations. Growth of muscle and active bone formation in the cubs probably accounted for their higher serum concentrations of creatinine and calcium, and higher activities of alkaline phosphatase. Only triglyceride concentrations varied between the sexes. The serum concentration of urea was higher than observed in other mustelids, consistent with a protein-rich diet and possibly related to the consumption of earthworms. PMID:18441351

Winnacker, H; Walker, N J; Brash, M G I; MacDonald, J A; Delahay, R J

2008-04-26

155

Hepatosplenic candidiasis, a late manifestation of Candida septicaemia in neutropenic patients with haematologic malignancies.  

PubMed

Systemic candidiasis with Candida-induced abscesses, predominantly in the liver and the spleen, was diagnosed in 27 patients with haematologic malignancies after intensive cytostatic therapy. Specific features included septic fever unresponsive to antimicrobial therapy, hepatosplenomegaly with multiple lesions in the liver and spleen (diameter up to 2 cm) as detected by computed tomography (CT) or ultrasound, and an elevation in liver enzymes. During treatment, induced neutropenia, hepatic and splenic foci were poorly defined histologically and were not identified by imaging procedures. After granulocyte recovery these foci showed characteristic histological patterns. Ultrasound and/or CT investigations of the abdomen now revealed characteristic lesions in the liver and the spleen. Gamma-GT and alkaline phosphatase were early indicators of hepatic involvement in Candida septicaemia and were often already elevated in aplasia. PMID:2337685

von Eiff, M; Essink, M; Roos, N; Hiddemann, W; Büchner, T; van de Loo, J

1990-04-01

156

Effects of bifenthrin on some haematological, biochemical and histopathological parameters of common carp (Cyprinus carpio L.).  

PubMed

The aim of this study was to assess the effect of bifenthrin on common carp (Cyprinus carpio L.). The 96-h LC50 value of Talstar EC 10 (active substance 100 g l(-1) bifenthrin) was found to be 57.5 microg l(-1). Examination of haematological and biochemical profiles and histological tissue examination was performed on common carp after 96 h of exposure to Talstar EC 10 (57.5 microg l(-1)). The experimental group showed significantly higher (P < 0.01) values of plasma glucose, ammonia, aspartate aminotransferase and creatine kinase as well as the relative and absolute monocyte count, compared with the control group. Histological examination revealed teleangioectasiae of secondary gill lamellae and degeneration of hepatocytes. The bifenthrin-based Talstar EC 10 pesticide preparation was classified as a substance strongly toxic for fish. PMID:18766454

Velisek, J; Svobodova, Z; Machova, J

2009-11-01

157

Tales from the Jazz ASH: highlights from the 2013 American Society of Haematology meeting.  

PubMed

The 55th annual ASH meeting was held in pleasant New Orleans and was the largest in its history, with 22,495 participants coming from 113 nations. A 'bench-to-bedside and back' attitude characterises haematology probably more than any other discipline in medicine and, as usual, this was reflected in the extremely wide breadth of the topics covered, including the last results from clinical trials and cutting-edge advancements in basic science. This year, the balance was arguably skewed: few truly clinical practice-changing results were presented. On the other hand, a great number of basic and translational studies significantly increased our understanding of the biology of numerous malignancies and heralded the coming of age of disruptive technologies. Namely, above all, next generation sequencing and T cell engineering-based cell therapy. PMID:24678345

Mazzarella, Luca

2014-01-01

158

Pathoanatomical aspects of malignant haematological disorders among Danish patients exposed to thorium dioxide.  

PubMed

The Danish Thorotrast Study was recently reestablished and improved. The cohort has been reidentified and followed up, and now comprises 1003 Thorotrast-exposed patients. For all suspected haematological cases, cytological and histological material has been revised and malignant diseases have been reclassified. The numbers of cases of leukemia and other related haematological disorders were as follows: 16 acute myeloid leukemia (AML); 8 myelodysplastic syndrome (MDS); 1 acute lymphatic leukemia (ALL); 3 chronic myeloid leukemia (CML); 4 non-Hodgkin's lymphoma (NHL); 2 multiple myeloma (MM); 2 myelofibrosis (MF); 2 chronic lymphatic leukemia (CLL). Except for CLL, all cases might be Thorotrast-induced. (Expected number of leukemias: < 2.5.) The findings in the German, Japanese, Portuguese and Danish studies are very similar. Some of the characteristic features include a high incidence of AML with several erythroleukemias, many cases of MDS, and a relatively low incidence of CML. In several studies of leukemia induced by alkylating agents, erythroleukemia is also described as a prominent feature. The possibility exists that a phase of relative predominance of erythroid elements in the bone marrow may be a common and not an unusual feature in the pathogenesis of these secondary leukemias. The findings are also compared with histopathological data from a Danish control group of de novo leukemia patients and from atomic bomb survivors with radiation-induced leukemia. The relative frequency of AML is higher among the Thorotrast-exposed patients than among the Danish control group and the A-bomb survivors. In contrast, low relative frequencies are seen for ALL and CML in Thorotrast cases in comparison with de novo leukemia cases and A-bomb survivors. It can be concluded that differences in relative and absolute frequency of leukemias and myelodysplastic syndrome exist not only between the irradiated populations and the unexposed control group, but even between groups exposed to low-LET (linear energy transfer) and high-LET radiation. PMID:7695889

Visfeldt, J; Andersson, M

1995-01-01

159

Exploring haematology nurses' perceptions of specialist education's contribution to care delivery and the development of expertise.  

PubMed

The role that educational preparation may play in the delivery of care and the development of expertise is a point of some debate [Manley, K., Garbett, R., 2000. Paying Peter and Paul reconciling concepts of expertise with competency for a clinical career structure. Journal of Clinical Nursing 9 (3), 347; King, L., Macleod Clark, J., 2002. Intuition and the development of expertise in surgical ward and intensive care nurses. Journal of Advanced Nursing 37(4), 322-329; Bonner, A., 2003. Recognition of expertise: an important concept in the acquisition of nephrology nursing expertise. Nursing & Health Sciences Journal 5, 123-131; Dunphy, B.C., Williamson, S.L., 2004. In pursuit of expertise. Advances in Health Sciences Education 9, 107-127]. Though education is a concept that may be universally valued, it may be more difficult to clearly discern the significance it has for practitioners who are developing their expertise. This research project employed an interpretive phenomenological design to explore the perceptions of specialist haematology nursing staff on the extent to which specialist education contributes to care delivery and the development of expert practice. A non-representative purposive sample of qualified nurses who had undertaken specialist education in haemopoiesis and work in specialist haematology participated in a focus group and semi-structured interviews. The report concludes that, for these specialist practitioners, specialist educational input had a beneficial impact on their levels of knowledge and confidence. Further to this, involvement in higher education had enabled them to become more active in the learning process. Perhaps the key finding of the study was the assertion by respondents that specialist educational input had enabled them to develop their specialist practice to a level that experience alone could not achieve. PMID:17126955

Atkinson, Joanne; Tawse, Stephen

2007-08-01

160

Biochemical and haematological changes in Sydney's the Sun City-to-Surf fun runners.  

PubMed

Before this study was undertaken, biochemical and haematological changes in collapsed fun runners have been interpreted only by comparison with changes observed in trained marathon runners, or by comparison with normal subjects. This study was undertaken to establish the pattern of biochemical and haematological changes to be expected in non-collapsed fun runners as a result of competition, and to use this pattern as the basis for comparison with changes observed in collapsed fun runners after similar competition. Forty-three fun runners (28 males, 15 females) who competed in the 1978 City-to-Surf run volunteered as non-collapsed "controls", while 10 runners (nine males, one female) who collapsed during the 1978 City-to-Surf run became "casualties" for comparison. Blood for detailed analysis was collected from the controls two weeks before the 1978 City-to-Surf run, immediately after the run and one month later; blood from the casualties was collected immediately after the run, and one month later. As a result of competition, three patterns of changes were observed, namely, haemoconcentration; tissue damage and altered organ system function; and alteration in carbohydrate metabolism. More intense tissue damage probably accounts for disproportionate changes of some variables in male controls compared with female controls, and in casualties compared with controls. Casualties differed most from controls in degree fo depression of serum bicarbonate levels, and degree of elevation of blood urea nitrogen, serum creatinine, uric acid and bilirubin levels. A wide scatter of blood sugar levels immediately after run both in controls and in casualties reflects the stress of prolonged exertion. PMID:522770

Richards, D; Richards, R; Schofield, P J; Ross, V

1979-11-01

161

Evaluacion hematologica en trabajadores expuestos a radiaciones ionizantes de un servicio de radioterapia. (Haematological evaluation in workers exposed to ionizing radiation from a radiotherapy service.).  

National Technical Information Service (NTIS)

Evaluation of haematological indicators is carried out as routing in medical surveillance of workers exposed to ionizing radiation, in order to assest the initial and continuing fitness for their intended tasks. The purpose of this work is to analize the ...

G. M. Lopez Pumar I. Frometa Suarez

1997-01-01

162

Guidelines for the prevention and treatment of infection in patients with an absent or dysfunctional spleen. Working Party of the British Committee for Standards in Haematology Clinical Haematology Task Force.  

PubMed Central

Overwhelming postsplenectomy infection should be preventable if simple precautions are taken. An ad hoc working party of the British Committee for Standards in Haematology has reviewed recommendations for patients without a spleen and drawn up a consensus. Members of the working party were selected for their personal expertise and to represent relevant professional bodies. The guidelines, which are set out below, include and extend the chief medical officer's 1994 update.

1996-01-01

163

Modulatory effect of N-acetylcysteine on pro-antioxidant status and haematological response in healthy men  

Microsoft Academic Search

The aim of this study was to follow up whether the modification of pro-antioxidant status by 8-day oral application of N-acetylcysteine\\u000a (NAC) in healthy men affects the haematological response, whether there is a direct relationship between antioxidant defences\\u000a and erythropoietin (EPO) secretion and whether NAC intake enhances exercise performance. Fifteen healthy men were randomly\\u000a assigned to one of two groups:

A. Zembron-Lacny; M. Slowinska-Lisowska; Z. Szygula; Z. Witkowski; K. Szyszka

2010-01-01

164

Age and sex-related differences in haematological values of captive white-tailed gnu ( Connochaetes gnou )  

Microsoft Academic Search

During an 8-year period (1980–1989), 199 samples were taken from 52 clinically healthy white-tailed gnu (Connochaetes gnou), 24 males and 28 females, to estimate basic haematological values and to find possible age- and sex-related differences.\\u000a In nine of the animals under investigation, four males and five females, the effect of sedating agents on blood parameters\\u000a was studied.\\u000a \\u000a A decrease of

J. Vahala; F. Kase

1993-01-01

165

Hema e-Chart registry of invasive fungal infections in haematological patients: improved outcome in recent years in mould infections.  

PubMed

The electronic surveillance system Hema e-Chart allowed us to prospectively collect data and to perform an analysis of invasive fungal infections (IFI) diagnosed in febrile patients as well as the procedures allowing their diagnosis and outcome according to the treatment given. Every patient admitted to 26 Italian Haematology Units with a new diagnosis of haematological malignancy and who was a candidate for chemotherapy was consecutively registered between March 2007 and March 2009. In all, 147 haematological patients with mycoses were identified. Yeasts were found in 23 infections; moulds were diagnosed in 17 proven, 35 probable and 72 possible mycoses. Galactomannan (GM) antigen was the most important test to diagnose probable mould infection; it was positive (cut-off >0.5) in 27 (77%) probable and in nine (53%) proven mould infections. Among patients with probable/proven mould infection who received no prophylaxis or non-mould-active prophylaxis with fluconazole, more patients (n = 26, 78.8%) had GM antigen positivity compared with patients (n = 10, 52.6%) given prophylaxis with mould-active drugs (p <0.05). First-line antifungal therapy was effective in 11/23 (48%) yeast infections and in 37/52 (71.2%) proven/probable mould infections. Twenty patients (14%) died within 12 weeks. The fungal attributable mortality was 30.4% and 17.3% in yeast and proven/probable mould infections, respectively. Among risk factors only age was independently associated (p 0.013) with mortality; sex, underlying haematological malignancy, previous prophylaxis and presence of neutropenia at diagnosis were not significant. A diagnosis of mould infection seemed to have a trend for a better outcome than the diagnosis of yeast infection (p 0.064). PMID:23279327

Nosari, A M; Caira, M; Pioltelli, M L; Fanci, R; Bonini, A; Cattaneo, C; Castagnola, C; Capalbo, S F; De Fabritiis, P; Mettivier, V; Morselli, M; Pastore, D; Aversa, F; Rossi, G; Pagano, L

2013-08-01

166

Protective effects of vitamin C against haematological and biochemical toxicity induced by deltamethrin in male Wistar rats  

Microsoft Academic Search

Deltamethrin is a synthetic pyrethroid insecticide. It is known for its wide toxic manifestations. The present experiment pertains to the protective role of vitamin C against haematological and biochemical toxicity induced by deltamethrin during 4 weeks. Male Wistar rats were divided into four groups of eight each: Group I served as control rats; Group II received deltamethrin (1.28mg\\/kg BW) in

Saoudi Mongi; Messarah Mahfoud; Boumendjel Amel; Jamoussi Kamel; El Feki Abdelfattah

2011-01-01

167

Haematological and serum biochemical profile of the upside-down catfish, Synodontis membranacea Geoffroy Saint Hilaire from Jebba Lake, Nigeria  

Microsoft Academic Search

Haematological and serum biochemical studies of natural population of Synodontis membranacea from Jebba Lake, North Central Nigeria were investigated in order to establish their mean and reference values. Bi-monthly\\u000a collection of 1,408 live fish samples was carried out between April 2002 and March 2004, using gill nets of various mesh sizes\\u000a ranging from 5.08 to 10.16 cm. The mean baseline value

Olufemi D. Owolabi

2011-01-01

168

Cytotoxic activity of calcein acetoxymethyl ester (calcein\\/AM) on primary cultures of human haematological and solid tumours  

Microsoft Academic Search

The aim of this study was to determine the in vitro cytotoxicity of calcein acetoxymethyl ester (Calcein\\/AM) on primary cultures derived from solid and haematological human tumours. Calcein\\/AM is a fluorescent dye that localises intracellularly after esterase-dependent cellular trapping and which has shown cytotoxic activity against various established human tumour cell lines at relatively low concentrations. The semi-automated fluorometric microculture

B. Jonsson; G. Liminga; K. Csoka; H. Fridborg; S. Dhar; P. Nygren; R. Larsson

1996-01-01

169

Increased risk of zoonotic Salmonella and Campylobacter gastroenteritis in patients with haematological malignancies: a population-based study  

Microsoft Academic Search

We hypothesised that haematological malignancies increase the risk of acquiring zoonotic Salmonella or Campylobacter gastroenteritis. The population-based study comprised all first-time Salmonella\\/Campylobacter gastroenteritis cases in two Danish counties (1991–2003), with age- and gender-matched controls from the background population.\\u000a We linked the study cohort to registries to obtain data on malignancies, chemotherapy (yes\\/no), and main comorbidities diagnosed\\u000a before Salmonella\\/Campylobacter gastroenteritis. Based

Kim O. Gradel; Mette Nørgaard; Claus Dethlefsen; Henrik C. Schønheyder; Brian Kristensen; Tove Ejlertsen; Henrik Nielsen

2009-01-01

170

Effect of Aqueous Extract of Telfairia occidentalis Leaf on the Performance and Haematological Indices of Starter Broilers.  

PubMed

This experiment was conducted to determine the effect of aqueous extract of Telfairia occidentalis (Fluted Pumpkin) leaf on the performance and haematological indices of starter broilers. A total of 200, 8-day-old broiler chicks were randomly allotted to five (5) treatments, each with 4 replicate groups containing 10 chicks and fed with standard starter broiler diets. Telfaria occidentalis leaves extract (FPLE) was added at 0, 40, 80, 120, and 160?mL/litre of drinking water. Growth performance and haematological indices were evaluated. Results showed that there was significant (P < 0.05) difference in weight gain, feed conversion, and protein efficiency ratios of the birds among the treatments. Birds fed 80?ml FPLE/litre of water had significantly the highest weight gain and the best feed conversion and protein efficiency ratios. There was no significant (P > 0.05) variations in the feed and water intakes of the birds. Results also show no significant (P > 0.05) difference in haematological indices of birds among the treatments. The results of this study indicate that, for enhanced weight gain and feed conversion efficiency, birds should be fed 80?mL FPLE/litre of water. PMID:23738128

P N, Onu

2012-01-01

171

Reference range variation in haematological indices amongst five different age groups of less than one year in Islamabad, Pakistan.  

PubMed

Objective: The objective of the current study was to establish the reference ranges of haematological indices amongst five healthy infantile (<1 year) age groups. Methodology: It was a descriptive cross sectional study carried out at the Department of Haematology, Armed Forces Institute of Pathology (AFIP), Rawalpindi. Non probability convenience sampling was adopted for the proceedings for the study. A sample size was 2000 which was equally distributed as 400 samples for all the five age groups i.e. <27 days, 3 month, 6 month, 9 month and one year. One thousands were males and 1000 were the females. An informed consent from the guardian was the pre requisite of study, while those candidates having an evidence of any systemic illness were not excluded. Results : The values of haematological indices i.e. MCV, MCHC, MCH, PCV and RDW varies with growing age of an infant. A decrease in all these values was observed from <27days to one year of age infants. Conclusion : A decrease in all these values was observed from <27days to one year of age infants. The values reported in this study can be used as a local reference for the newborn aged between <27 days and 1 year of age. PMID:24353580

Tauseef Bukhari, Kiran; Zafar, Humaira

2013-04-01

172

Reference range variation in haematological indices amongst five different age groups of less than one year in Islamabad, Pakistan  

PubMed Central

Objective: The objective of the current study was to establish the reference ranges of haematological indices amongst five healthy infantile (<1 year) age groups. Methodology: It was a descriptive cross sectional study carried out at the Department of Haematology, Armed Forces Institute of Pathology (AFIP), Rawalpindi. Non probability convenience sampling was adopted for the proceedings for the study. A sample size was 2000 which was equally distributed as 400 samples for all the five age groups i.e. <27 days, 3 month, 6 month, 9 month and one year. One thousands were males and 1000 were the females. An informed consent from the guardian was the pre requisite of study, while those candidates having an evidence of any systemic illness were not excluded. Results : The values of haematological indices i.e. MCV, MCHC, MCH, PCV and RDW varies with growing age of an infant. A decrease in all these values was observed from <27days to one year of age infants. Conclusion : A decrease in all these values was observed from <27days to one year of age infants. The values reported in this study can be used as a local reference for the newborn aged between <27 days and 1 year of age.

Tauseef Bukhari, Kiran; Zafar, Humaira

2013-01-01

173

Prevalence of Plasmodium falciparum Parasitaemia and Its Correlation with Haematological Parameters among HIV-Positive Individuals in Nigeria  

PubMed Central

Malaria and HIV are the two most important health challenges of our time. Haematologic abnormalities are features in Plasmodium falciparum infection, and anaemia is a well-known outcome. The prevalence and haematological impact of P. falciparum parasitaemia were determined among HIV-infected individuals in Nigeria. Parasite detection was carried out using microscopy and Polymerase Chain Reaction (PCR). Haemoglobin concentration was determined using an automated machine while CD4+ T-cells count was analyzed using flow cytometer. Thirty-seven (18.5%) out of the 200 HIV individuals enrolled had malaria parasites detected in their blood. All the positive cases were detected by PCR while only 20 (10%) were detected by thick blood microscopy. The mean haemoglobin concentration and packed cell volume (PCV) of HIV individuals with malaria parasitaemia were lower compared to those without malaria parasitaemia but the difference was not statistically significant. Also no significant difference was observed in malaria positivity in respect to sex and mean CD4+ cell count. The study highlights the effects of P. falciparum parasitaemia on the haematologic and immune components of HIV individuals.

Ojurongbe, Olusola; Oyeniran, Oluwatoyin Adeola; Alli, Oyebode Armstrong Terry; Taiwo, Sunday Samuel; Ojurongbe, Taiwo Adetola; Olowe, Adekunle Olugbenga; Opaleye, Oluyinka Oladele; Adeyeba, Oluwaseyi Adegboyega

2014-01-01

174

Multivitamin supplementation improves haematologic status in children born to HIV-positive women in Tanzania  

PubMed Central

Introduction Anaemia is prevalent among children born to HIV-positive women, and it is associated with adverse effects on cognitive and motor development, growth, and increased risks of morbidity and mortality. Objective To examine the effect of daily multivitamin supplementation on haematologic status and mother-to-child transmission (MTCT) of HIV through breastfeeding. Methods A total of 2387 infants born to HIV-positive women from Dar es Salaam, Tanzania were enrolled in a randomized, double-blind, placebo-controlled trial, and provided a daily oral supplement of multivitamins (vitamin B complex, C and E) or placebo at age 6 weeks for 24 months. Among them, 2008 infants provided blood samples and had haemoglobin concentrations measured at baseline and during a follow-up period. Anaemia was defined as haemoglobin concentrations<11 g/dL and severe anaemia<8.5 g/dL. Results Haemoglobin concentrations among children in the treatment group were significantly higher than those in the placebo group at 12 (9.77 vs. 9.64 g/dL, p=0.03), 18 (9.76 vs. 9.57 g/dL, p=0.004), and 24 months (9.93 vs. 9.75 g/dL, p=0.02) of follow-up. Compared to those in the placebo group, children in the treatment group had a 12% lower risk of anaemia (hazard ratio (HR): 0.88; 95% CI: 0.79–0.99; p=0.03). The treatment was associated with a 28% reduced risk of severe anaemia among children born to women without anaemia (HR: 0.72; 95% CI: 0.56–0.92; p=0.008), but not among those born to women with anaemia (HR: 1.10; 95% CI: 0.79–1.54; p=0.57; p for interaction=0.007). One thousand seven hundred fifty three infants who tested HIV-negative at baseline and had HIV testing during follow-up were included in the analysis for MTCT of HIV. No association was found between multivitamin supplements and MTCT of HIV. Conclusions Multivitamin supplements improve haematologic status among children born to HIV-positive women. Further trials focusing on anaemia among HIV-exposed children are warranted in the context of antiretroviral therapy.

Liu, Enju; Duggan, Christopher; Manji, Karim P; Kupka, Roland; Aboud, Said; Bosch, Ronald J; Kisenge, Rodrick R; Okuma, James; Fawzi, Wafaie W

2013-01-01

175

Assessment of haematological parameters in HIV-infected and uninfected Rwandan women: a cross-sectional study  

PubMed Central

Objectives Although haematological abnormalities are common manifestations of HIV infection, few studies on haematological parameters in HIV-infected persons have been undertaken in sub-Saharan Africa. The authors assessed factors associated with haematological parameters in HIV-infected antiretroviral-naïve and HIV-uninfected Rwandan women. Study design Cross-sectional analysis of a longitudinal cohort. Setting Community-based women's associations. Participants 710 HIV-infected (HIV+) antiretroviral-naïve and 226 HIV-uninfected (HIV?) women from the Rwanda Women's Interassociation Study Assessment. Haematological parameters categorised as (abnormal vs normal) were compared by HIV status and among HIV+ women by CD4 count category using proportions. Multivariate logistic regression models using forward selection were fit. Results Prevalence of anaemia (haemoglobin (Hb) <12.0?g/dl) was higher in the HIV+ group (20.5% vs 6.3%; p<0.001), and increased with lower CD4 counts: ?350 (7.6%), 200–349 (16%) and <200 cells/mm3 (32.2%). Marked anaemia (Hb <10.0?g/dl) was found in 4.2% of HIV+ and none of the HIV? women (p<0.001), and was highest in HIV+ women with CD4 <200 cells/mm3 (8.4%). The HIV+ were more likely than HIV? women (4.2 vs 0.5%, respectively, p=0.002) to have moderate neutropenia with white blood cells <2.0×103 cells/mm3 and 8.4% of HIV+ women with CD4 <200 cells/mm3 had moderate neutropenia. In multivariate logistic regression analysis, BMI (OR 0.87/kg/m2, 95% CI 0.82 to 0.93; p<0.001), CD4 200–350 vs HIV? (OR 3.59, 95% CI 1.89 to 6.83; p<0.001) and CD4 <200 cells/mm3 vs HIV? (OR 8.09, 95% CI 4.37 to 14.97; <0.001) had large independent associations with anaemia. There were large independent associations of CD4 <200 cells/mm3 vs HIV? (OR 7.18, 95% CI 0.78 to 65.82; p=0.081) and co-trimoxazole and/or dapsone use (OR 5.69, 95% CI 0.63 to 51.45; p=0.122) with moderate neutropenia. Conclusions Anaemia was more common than neutropenia or thrombocytopenia in the HIV-infected Rwandan women. Future comparisons of haematological parameters in HIV-infected patients before and after antiretroviral therapy initiation are warranted.

Munyazesa, Elisaphane; Emile, Ivan; Mutimura, Eugene; Hoover, Donald R; Shi, Qiuhu; McGinn, Aileen P; Musiime, Stephenson; Muhairwe, Fred; Rutagengwa, Alfred; Dusingize, Jean Claude; Anastos, Kathryn

2012-01-01

176

Effect of azadirachtin on haematological and biochemical parameters of Argulus-infested goldfish Carassius auratus (Linn. 1758).  

PubMed

Argulosis hampers aquaculture production and alters the host physiology and growth. Azadirachtin is recognized as a potential antiparasitic agent against Argulus sp. The present study aimed to investigate the effect of different concentration of azadirachtin solution on haematological and serum biochemical parameters of Argulus-infested goldfish Carassius auratus. Ninety Argulus-infested goldfish were randomly divided into six equal groups. Fish of group 1-5 were treated with azadirachtin solution through bath of 1, 5, 10, 15 and 20 mg L(-1) as T1, T2, T3, T4 and T5, respectively, and group 6 was exposed to 2% DMSO solution without azadirachtin and considered as negative control T0(-). Along with six treatment groups, a positive control T0(+) of healthy goldfish free from Argulus infestation was also maintained. Parasitic mortality was evaluated after 3 days of consecutive bath treatment. After 7 days of post-treatment, the blood and serum were drawn from each of the treatment groups and haematological and serum biochemical parameters were evaluated. Total leucocyte count (TLC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), blood glucose, total protein (TP), globulin, serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) were significantly (p < 0.05) high in negative control group when compared with positive control group. It could be concluded that Argulus infestation altered marked haematological and serum biochemical parameters. However, in treated groups complete elimination of Argulus was found in T4 and T5 groups. Also significant (p < 0.05) reduction in haematological and serum biochemical parameters of all the treatment groups were recorded in comparison with negative control group. In addition, T4 and T5 groups showed significantly (p < 0.05) high superoxide dismutase (SOD), catalase, total erythrocyte count (TEC) and haemoglobin (Hb). However, higher mean corpuscular haemoglobin concentration (MCHC), blood glucose and lactate dehydrogenase (LDH) levels in T5 group revealed that higher concentration of azadirachtin have notable effects on activity of vital tissues function and physiology of the host. Argulus spp. from infested goldfish could be eliminated using bath treatment with solution of azadirachtin having concentration of 15 mg L(-1) and that also shifted haematological and serum biochemical parameters towards homeostasis. PMID:23090629

Kumar, Saurav; Raman, R P; Kumar, Kundan; Pandey, P K; Kumar, Neeraj; Mallesh, B; Mohanty, Snatashree; Kumar, Abhay

2013-08-01

177

Optimising a curriculum for clinical haematology and biochemistry in sports medicine: a Delphi approach  

PubMed Central

Objectives To investigate issues of curriculum in the context of a postgraduate sports medicine training programme, specifically in the field of clinical biochemistry and haematology. Methods Following the Delphi methodology, a series of sequential questionnaires was administered to curriculum developers, clinical teachers, examiners, and registrars. Results Agreement on a core syllabus for this subject with an indication of the core aims and objectives of teaching and learning in this field and the associated required skills and competencies. An indication of current and ideal teaching and learning methods and reasons for these preferences. A consensus of key features of a teaching module for this field and of appropriate methods of examination. Conclusions The data derived from this study, as well as the experience of engaging in it, will better inform curriculum developers, teachers, and students of one another's perceptions as to what is important in and appropriate to teaching and learning in this field of sports medicine. Engagement in the exercise and broader consideration of the outcomes by those who develop the curriculum, teach, and formulate the examination process will facilitate attainment of the ideal of well aligned teaching, learning, and examination in this specific field.

Fallon, K E; Trevitt, A C

2006-01-01

178

To what extent will contaminated water affect physical, haematological and lipid properties of Clariasgariepinus?  

PubMed

Some haematological and physical properties of Clarias gariepinus cultivated in water contaminated with phthalate, benzene and cyclohexane over a period of 56 days were examined. The haemoglobin (Hb) and packed cell volume (PCV) concentrations of test animals were found to be significantly lower than those of control (p<0.05). The gain in body weight of male C. gariepinus cultivated in uncontaminated water (control), water contaminated with phthalate, benzene and cyclohexane was found to be 11.88 ± 0.55, 13.5 ± 0.62, 21.60 ± 1.03 and 27.54 ± 1.22 g, respectively. Serum cholesterol concentrations of fish cultivated in contaminated water were found to be significantly higher than those of control (p<0.05). The experimental results suggested that the pollutants may cause anaemia in the fish as evident in the Hb and PCV results. It could also be inferred that the gain in body weight may be due to impaired fat metabolism which might have led to elevated levels of serum cholesterol and triglycerides in the fish. It is our view that consumption of such fish may cause hypercholesterolemia. PMID:19801186

Adeyemi, Olalekan; Oginni, Olatunde; Igbakin, Ayanfe P; Adeyemi, Oyeyemi; Osubor, Chris C

2011-01-01

179

Berberine-induced haemolysis revisited: safety of Rhizoma coptidis and Cortex phellodendri in chronic haematological diseases.  

PubMed

Two commonly used berberine-containing Chinese herbs, Rhizoma coptidis (RC) and Cortex phellodendri (CP), have been banned in Singapore for the past three decades due to implication of berberine in aggravating jaundice and kernicterus in neonates with glucose-6-phosphate dehydrogenase deficiency. Here we conducted a single arm, phase I/II clinical study on Chinese herbal medicine for patients with chronic cytopenic haematological conditions and we analysed a subset of 20 patients who also had RC, CP or both in their herbal concoction. We found no organ toxicity or electrolyte imbalance in these 20 patients where RC was administered for 1055 patient-days and CP for 1252 patient-days. In three patients with thalassemia intermedia, transient elevation in serum bilirubin level was observed but this was not associated with any aggravation of anaemia or liver dysfunction. A review of the literature found conflicting evidence of varying levels either supporting or refuting the allegation of neonatal jaundice and kernicterus caused by berberine. There were, however, very few clinical reports of adverse reaction attributable to RC or CP in oral TCM concoction. We conclude that based on traditional dosage and indication, the use of RC and CP in oral concoction is safe. PMID:22002596

Linn, Yeh-ching; Lu, Jiahui; Lim, Lay-Cheng; Sun, Huili; Sun, Jue; Zhou, Yongming; Ng, Han-seong

2012-05-01

180

[Inclusion Body Disease (IBD of Boids)--a haematological, histological and electron microscopical study].  

PubMed

Our objective was to evaluate diagnostic tools for the detection of Inclusion Body Disease (IBD) in bold snakes. The aetiology of IBD is unknown, and the disease has non-specific clinical signs, hence there is a need for a clinically-applicable, specific diagnostic method. We examined blood smears and liver biopsies from 26 bold snakes (17 boas and nine pythons; some of which were suspected of having IBD) for the presence of characteristic inclusion bodies. We used haematology, histology and electron microscopy to characterise samples as IBD-positive or -negative. Our results indicate that examination of a simple blood smear is sufficient to diagnose IBD in boas. Inclusion bodies in lymphocytes, erythrocytes and thrombocytes were observed. In both, boas and pythons, we detected inclusion bodies within hepatocytes. We demonstrated also that IBD was more common in boas than in pythons: only samples from two Ball Pythons (Python regius) tested positive, whereas no other Pythonidae were positive. We consider that blood smears represents a rapid, non-invasive technique for detection of IBD. PMID:23045804

Keilwerth, Melanie; Bühler, Ilina; Hoffmann, Rudolf; Soliman, Hatem; El-Matbouli, Mansour

2012-01-01

181

Specificity and sensitivity of immunocytochemistry for detecting P-glycoprotein in haematological malignancies.  

PubMed Central

AIMS--To determine the optimal working conditions of the alkaline phosphatase-antialkaline phosphatase (APAAP) method to establish a specific and sensitive assay for the detection of low numbers of MDR positive cells in patients with hematological malignancies. METHODS--Three monoclonal antibodies (C-219, JSB-1, MRK-16) were used for the detection of P-glycoprotein (P-gp) in cell lines and in samples from 43 patients with haematological malignancies. The results of the APAAP method were compared with western blotting for specificity and sensitivity. RESULTS--Excellent correlation was obtained between optimised APAAP and western blotting, except in the case of multiple myeloma. JSB-1 seemed to be the more useful monoclonal antibody for the APAAP which was more sensitive than western blotting in its ability to detect single P-gp positive cells. CONCLUSIONS--Methods for P-gp detection, as defined by multidrug resistant (MDR) cell lines, are not necessarily optimal and specific for clinical samples and may lead to higher false positive and negative results, according to the conditions and the monoclonal antibodies used. Images

Gala, J L; McLachlan, J M; Bell, D R; Michaux, J L; Ma, D D

1994-01-01

182

Effects of tiletamine-zolazepam anaesthesia on plasma antioxidative status and some haematological parameters in sheep.  

PubMed

It is not clear whether the anaesthetic agents tiletamine and zolazepam have antioxidant or pro-oxidant effects. Therefore, this study was carried out to investigate the effects of tiletamine-zolazepam anaesthesia on oxidant/antioxidant status in blood plasma and on haematological parameters in 10 healthy Awassi ewes. The tiletamine-zolazepam combination was administrated in a dose of 7.5 mg/kg intramuscularly. The animals were spontaneously breathing air during the procedure. Blood samples were collected by jugular venipuncture before induction and at 30, 60, 120 min, 24 h and 3 days after anaesthesia. Malondialdehyde concentration, an index of lipid peroxidation, was higher at 30, 60, 120 min and 24 h (P < 0.05) than the baseline value in the plasma. The level of glutathione decreased (P < 0.05) at 30, 60 and 120 min, then returned to the baseline level. Beta-carotene concentration was lower (P < 0.05) than the baseline value during anaesthesia with the exception of its level at 120 min. Glutathione peroxidase and catalase activities decreased (P < 0.05) at the onset of anaesthesia, then returned to baseline values. There was no significant change in vitamin A level. Red blood cell count, haematocrit and haemoglobin concentration significantly decreased (P < 0.05) only at 30 min and thereafter they gradually returned to the baseline values. Based on the results tiletamine-zolazepam anaesthesia seems to accelerate lipid peroxidation and to impair the enzymatic antioxidant defence in the blood plasma. PMID:17555283

Ceylan, C; Aydilek, N; Ipek, H

2007-06-01

183

Effect of copper deficiency on blood lipid profile and haematological parameters in broilers.  

PubMed

The aim of the study was to observe the changes in haematological parameters and blood lipid profile through copper (Cu) deficiency. Eighty broiler chicks were used in the study. The chicks were separated into two equal groups (n = 40), feed treatment according to the groups started on day 7. The animals in the control group were fed with normal feed. Those in the Cu-deficient group were fed with feed that did not contain supplemental Cu. Blood samples were taken from all the animals on days 7, 21 and 49. Fibrinogen and prothrombin time were assayed in plasma; total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol (LDL-cholesterol), triglyceride and ceruloplasmin were assayed in sera; and haematocrit and thrombocyte levels were assayed in the blood samples. Total cholesterol, triglyceride and LDL-cholesterol levels were significantly higher in the Cu-deficient group than in the control group. Serum ceruloplasmin levels were lower in the Cu-deficient group than in the control group. Prothrombin time was higher in the Cu-deficient group than in the control group. Haematocrit levels were lower in the Cu-deficient group than in the control group. It was concluded that Cu deficiency created hypertriglyceridaemia, hypercholesterolaemia, insufficient growth and anaemia in broilers. PMID:16970628

Kaya, A; Altiner, A; Ozpinar, A

2006-10-01

184

Atlas of Genetics and Cytogenetics in Oncology and Haematology in 2013  

PubMed Central

The Atlas of Genetics and Cytogenetics in Oncology and Haematology (http://AtlasGeneticsOncology.org) is a peer-reviewed internet journal/encyclopaedia/database focused on genes implicated in cancer, cytogenetics and clinical entities in cancer and cancer-prone hereditary diseases. The main goal of the Atlas is to provide review articles that describe complementary topics, namely, genes, genetic abnormalities, histopathology, clinical diagnoses and a large iconography. This description, which was historically based on karyotypic abnormalities and in situ hybridization (fluorescence in situ hybridization) techniques, now benefits from comparative genomic hybridization and massive sequencing, uncovering a tremendous amount of genetic rearrangements. As the Atlas combines different types of information (genes, genetic abnormalities, histopathology, clinical diagnoses and external links), its content is currently unique. The Atlas is a cognitive tool for fundamental and clinical research and has developed into an encyclopaedic work. In clinical practice, it contributes to the cytogenetic diagnosis and may guide treatment decision making, particularly regarding rare diseases (because they are numerous and are frequently encountered). Readers as well as the authors of the Atlas are researchers and/or clinicians.

Huret, Jean-Loup; Ahmad, Mohammad; Arsaban, Melanie; Bernheim, Alain; Cigna, Jeremy; Desangles, Francois; Guignard, Jean-Christophe; Jacquemot-Perbal, Marie-Christine; Labarussias, Maureen; Leberre, Vanessa; Malo, Anne; Morel-Pair, Catherine; Mossafa, Hossein; Potier, Jean-Claude; Texier, Guillaume; Viguie, Franck; Yau Chun Wan-Senon, Sylvie; Zasadzinski, Alain; Dessen, Philippe

2013-01-01

185

Prenatal ultrasound heating impacts on fluctuations in haematological analysis of Oryctolagus cuniculus  

PubMed Central

Prenatal Ultrasound (US) is commonly used as a routine procedure on pregnant women. It is generally perceived as a safe procedure due to the use of non-ionizing radiation. However, the neurotoxicity of diagnostic prenatal US was detected to have a correlation with high susceptibility to early developing fetus. This research involved in vivo experimental model by using 3rd trimester pregnant Oryctolagus cuniculus and exposing them to US exposures for 30, 60, and 90 minutes at their gestational day (GD) 28-29. The output power and intensities, spatial peak temporal average intensity (ISPTA) of US were varied from 0.4 to 0.7 W and 0.13 to 0.19 W/cm2 respectively were tested initially in free-field, water. Haematological analysis was carried out to detect any changes in blood constituents. Statistically significant differences were detected in red blood cell (RBC) count (P<0.001), haemoglobin (Hb) concentration (P<0.001) and also platelet (PLT) count (P<0.001) in newborn of Oryctolagus cuniculus. These findings indicate the possibility of US heating in causing defects on studied animal.

Md. Dom, Sulaiman; Abdul Razak, Hairil Rashmizal; Hassan, Hamzah Fansuri

2013-01-01

186

Psychosocial factors associated with impact of cancer in longterm haematological cancer survivors.  

PubMed

To assess the impact of cancer (IOC) on subsequent quality of life (QOL), 718 long-term haematological cancer survivors completed validated psychosocial, functional and QOL scales, including IOC. Fifteen percent reported significant psychological distress, 18% high levels of fatigue and 10% moderate to severe functional impairment. These groups of participants also showed poorer QOL. There were no significant differences in psychological distress (P = 0·76), fatigue (P = 0·23) or functional impairment (P = 0·74) across different cancer subtypes. Two separate hierarchical regression analyses examined the combined association of disease-type, psychosocial and other factors on negative and positive IOC scores respectively. Higher negative IOC scores were significantly associated (P ? 0·001) with medical comorbidity, psychological distress, lower social support, high fatigue levels and functional impairment. Paediatric patients (diagnosed at <17 years) had significantly higher negative IOC scores than adult patients (P = 0·001); greater years since diagnosis was significantly (P < 0·001) associated with less negative IOC. Higher positive IOC was associated with acute leukaemia (P = 0·01); lower positive IOC with paediatric patients (P < 0·001), white ethnicity (P < 0·001), higher education (P = 0·003), no partner (P = 0·01) and lower social support (P = 0·01). Screening for medical comorbidity, psychological distress and fatigue identifies those needing most support and should allow earlier interventions to address negative and positive IOC to improve the well-being of cancer survivors. PMID:24372352

Korszun, Ania; Sarker, Shah-Jalal; Chowdhury, Kashfia; Clark, Charlotte; Greaves, Paul; Johnson, Rachel; Kingston, Judith; Levitt, Gill; Matthews, Janet; White, Peter; Lister, Andrew; Gribben, John

2014-03-01

187

Comparative haematological changes following Trypanosoma vivax and T. congolense infections in Zebu bulls.  

PubMed

A comparative study of haematological changes subsequent to Trypanosoma vivax and Trypanosoma congolense infections was carried out using 24 Zebu bulls during a period of 12 weeks. Eight bulls were infected with T. vivax, another eight with T. congolense and eight served as controls. Infected bulls developed chronic trypanosomiasis which was characterized by many clinical manifestations including intermittent pyrexia. Elevated rectal temperatures of up to 105 and 106 degrees F were recorded, respectively, in all bulls infected with T. vivax or T. congolense. Mean parasitaemia was higher and more chronic in T. congolense-infected bulls and ranged between means of 0 and 3.06 in all infected bulls. There was a slight and transient drop in packed cell volume (PCV) of T. vivax-infected bulls as against a marked and more chronic drop in T. congolense-infected bulls. Mean PCV of T. vivax- and T. congolense-infected bulls and controls ranged between 28 and 38, 17 and 38, and 31 and 38%, respectively. Haemoglobin (Hb) concentrations also decreased in infected bulls. The decrease was greater and more chronic in the T. congolense-infected bulls. Values ranged between means of 10.47 and 13.84, 5.44 and 14.16, and 10.24 and 14.12 g dl-1 in T. vivax- and T. congolense-infected and control bulls, respectively. Total plasma proteins also decreased in infected bulls; this was more marked in the T. congolense-infected group. Values for T. vivax-infected, T. congolense-infected and control bulls ranged between means of 7.66 and 8.99, 6.26 and 8.81, and 7.94 and 8.78 g dl-1, respectively.2+ that the indigenous T. vivax strains are more PMID:2343522

Sekoni, V O; Saror, D I; Njoku, C O; Kumi-Diaka, J; Opaluwa, G I

1990-02-01

188

Clinical and haematological characterisation of Mycoplasma suis infections in splenectomised and non-splenectomised pigs.  

PubMed

Mycoplasma suis causes infectious anaemia in pigs (IAP), which can manifest in various degrees of severity depending on the virulence and the host's susceptibility. As M. suis cannot be cultured in vitro experimental infections of splenectomised animals play an essential role for pathogenesis research. The aim of the present study was to characterise the course of experimental infection using the highly virulent and red blood cell (RBC-) invasive M. suis strain KI3806, to compare the experimental course in splenectomised and non-splenectomised pigs and to correlate clinical and haematological parameters with M. suis blood loads. All infected splenectomised pigs (n=7) were PCR-positive 2 days post infection (DPI) with maximum mean bacterial loads of 1.61×10(10)M. suis/mL on 8 DPI. They developed severe anaemia and massive hypoglycaemia by 8 DPI and had to be euthanised preterm (until 8 DPI) without seroconversion. The non-splenectomised pigs (n=7) became PCR-positive within 23 DPI and reached a maximum mean M. suis load of 1.64×10(5)M. suis/mL on 8 DPI. They developed mild anaemia, massive skin alterations with petechiae and haemorrhagic diathesis and seroconverted within 35 DPI. The study demonstrated that experimental infection of splenectomised pigs with the highly virulent M. suis strain KI3806 induces a fulminant course of infection. In contrast, M. suis strain KI3806 induces a mild course of disease in non-splenectomised pigs, which resembles the situation in naturally infected pigs. Therefore, these infection models are valuable for future pathogenesis studies on acute and chronic M. suis infections. PMID:24933162

Stadler, J; Jannasch, C; Mack, S L; Dietz, S; Zöls, S; Ritzmann, M; Hoelzle, K; Hoelzle, L E

2014-08-01

189

Distribution of haematological indices among subjects with Blastocystis hominis infection compared to controls  

PubMed Central

Introduction Some studies suggest Blastocystis hominis is a potentially pathogenic protozoa. Blastocystis hominis contributed to anaemia in children aged 8–10 years old in one study. Aim To compare haematological indices in cases with blastocystis hominis infection with healthy controls. Material and methods From 2001 to 2012, 97600 stool examinations were done in 4 university hospitals. Parasites were observed in 46,200 specimens. Of these cases, subjects with complete laboratory investigation (complete blood count – CBC, ferritin, total iron binding capacity – TIBC, and serum) and blastocystis hominis infection were included in this study as the case group. Of these cases, 6851 cases had only B. hominis infection. In the control group, 3615 subjects without parasite infestation were included. Age, haemoglobin (Hb), serum iron, TIBC, white blood cell (WBC), platelet (PLT), mean corpuscular volume (MCV), haematocrit (HCT) and erythrocyte sedimentation rate (ESR) were recorded for cases and controls. SPSS software version 13.0 was used for analysis. Independent sample t-test and ?2 tests were used for comparison. Results Erythrocyte sedimentation rate level was significantly higher in cases with B. hominis infection (p < 0.05). C-reactive protein level was positive in 1.46% of cases and 0.5% of controls, which was statistically significant (p < 0.05). Frequency of serum iron < 120 was significantly higher in cases with B. hominis infection compared to controls. Occult blood was positive in 0.93% of cases and in none of the controls (p < 0.05). Conclusions The ESR, CRP and occult blood was significantly higher in cases with B. hominis infection.

Javaherizadeh, Hazhir; Soltani, Shahrzad; Torabizadeh, Mehdi; Yousefi, Elham

2014-01-01

190

Fish and fish oil intake: effect on haematological variables related to cardiovascular disease.  

PubMed

To investigate the effects of practical amounts of n-3 highly-unsaturated fatty acids (HUFA) on a number of parameters involved in haemostasis, 12 healthy men were fed three diets in a 3 x 3 cross-over design. The diets, fed as the evening meal for 6-week periods, were: Control diet (essentially fish-free), Fish diet (200 g/d of lean Australian fish flesh) and the same fish-based diet but supplemented with 5 g/d fish oil (Fish + Oil). The diets supplied about 0, 0.6, and 2.0 g n-3 HUFA/d, respectively. Relative to the Control diet, the number of circulating leukocytes was significantly reduced after both the Fish and Fish + Oil diets (by 13% and 15%, respectively). This reduction occurred in the number of neutrophils, although this did not reach significance on the Fish diet alone. Platelet count fell on the Fish + Oil diet (by 6%) but not on the Fish diet alone. There was an apparent enhancement of fibrinolytic potential after both fish-containing diets, which tended to be accentuated with the fish oil supplementation. The mechanisms underlying some if not all of these observations may be eicosanoid-mediated as indicated by a diminution in the platelet arachidonic acid (20:4n-6) to eicosapentaenoic acid (20:5n-3) ratios after the Fish + Oil (13.7 +/- 1.8) and Fish (34.4 +/- 8.9) diets relative to the Control diet (66.1 +/- 15.6). These data suggest that a practical amount of lean fish can improve certain haematological parameters implicated in the etiology of cardiovascular disease. PMID:1811337

Brown, A J; Roberts, D C

1991-10-15

191

Effects of sodium butyrate on growth performance, haematological and immunological characteristics of weanling piglets.  

PubMed

The experiment was conducted to study the effects of sodium butyrate (SB) on growth, haematological and immunological characteristics in weanling pigs. A total of 100 male piglets (Duroc×Landrace×Yorkshire) with a body weight of 8.0 ± 0.2 kg weaned at the age of 28 days were randomly assigned to two treatments with five replicates and 10 pigs per replicate. Piglets received a basal diet (control group) or diets supplemented with 1000 mg/kg SB. The feeding trial lasted for 21 days. The results showed that dietary SB significantly decreased (p < 0.05) diarrhoea incidence of weaned piglets, but did not affect (p > 0.05) the average daily feed intake (ADFI), average daily gain (ADG) and feed to gain (F/G). Furthermore, piglets fed dietary SB had higher (p < 0.05) serum concentrations of glucose and triglycerides and lower (p < 0.05) serum concentrations of urea nitrogen, cortisol, D-lactic acid and diamine oxidase when compared with the control group. However, dietary SB did not affect concentrations of serum albumin, total protein, insulin and glucagon (p > 0.05). There were no significant (p > 0.05) treatment effects on serum IgA and IgM, whereas serum IgG concentration and IgA+ cell count in jejunum from pigs fed SB were significantly higher (p < 0.05) than in those given the basal diet. In conclusion, the present study indicated that dietary SB significantly decreased diarrhoea incidence of weaned piglets and increased the efficiency of nitrogen utilization. Also, dietary SB could regulate and enhance the immune function of piglets by increasing the serum IgG concentration and IgA+ cell count in jejunum. Our results suggest that SB may reduce some of the adverse effects of weaning stress and play an important role in maintaining the integrity of intestinal mucosa. PMID:24024579

Fang, C L; Sun, H; Wu, J; Niu, H H; Feng, J

2014-08-01

192

Effects of additive iron on growth, tissue distribution, haematology and immunology of gilthead sea bream, Sparus aurata  

Microsoft Academic Search

The comparative effects of iron-supplemented levels on growth, tissue distribution, haematology and immunology of gilthead\\u000a sea bream, Sparus aurata (2 g) were investigated, using four organic (50, 100, 200, 300 mg ORG\\/kg diet) and one inorganic iron source (200 INOR mg\\/kg diet).\\u000a Fish were treated for 12 weeks with the experimental diets and maintained at a water temperature of 19–22°C. Growth (final\\u000a weight and specific growth

George RigosAlexandros; Alexandros Samartzis; Morgane Henry; Eleni Fountoulaki; Efthimia Cotou; John Sweetman; Simon Davies; Ioannis Nengas

2010-01-01

193

Some changes in the haematological parameters of rainbow trout ( Oncorhynchus mykiss ) exposed to three artificial photoperiod regimes  

Microsoft Academic Search

Haematological parameters are often used as health status and stress indicators in fish. However, information on the effects\\u000a of artificial photoperiods on these parameters is scarce and ambiguous. The consequences of three different artificial photoperiod\\u000a regimes [light\\/day 12 h:12 h (LD 12:12) for 150 days as controls; LD 14:10 and LD 24:0, both for an initial 60 days, and then\\u000a LD 12:12 for the remaining 90 days] were evaluated

A. E. Valenzuela; V. M. Silva; A. E. Klempau

2007-01-01

194

Are published standards for haematological indices in pregnancy applicable across populations: an evaluation in healthy pregnant Jamaican women  

PubMed Central

Background The haematological profile of the pregnant woman has an impact on the outcome of the pregnancy. Published guidelines indicate acceptable levels for haematological indices in pregnancy but they are population specific. Indicators of haemoglobin concentration are the most commonly utilized of the indices. These published international norms are used across populations, however, there is no evidence confirming their applicability to a population such as the Jamaican pregnant woman. This study was therefore undertaken with the intent of documenting the haematological profile of pregnant primigravid Jamaican women and comparing these to the established norms to determine whether the norms apply or whether there was a need to establish local norms. Methods This was a longitudinal study done on a cohort of 157 healthy primigravid women ages 15 to 25 and without anaemia, and who were recruited from the antenatal clinic of the University Hospital of the West Indies, Kingston, Jamaica. The haemoglobin concentration, packed cell volume, mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, white blood cell count, red blood cell count and platelet count were measured on samples of blood obtained from each consenting participant during each of the three trimesters. The results were analysed using SPSS for windows (Version 11) and the data expressed as means ± S.D. Means were compared using the student's paired t-test. Comparison was then made with the international norms as recommended by the United States Center for Disease Control (1989). Ethical approval for this study was obtained from the University Hospital of the West Indies/University of the West Indies Ethics Committee. Results The results showed changes by trimester in all measured variables. For most of the indices the changes achieved levels of significance across trimesters. These changes were however in keeping with the expected physiological response in pregnancy and the values were similar to the published international norms. Conclusion The findings suggest that the international norms for haematological indices in pregnancy are applicable across populations and to the pregnant Jamaican primigravid woman. This finding may be reassuring to others with a similar population and stage of development as Jamaica.

James, Tameika R; Reid, Harvey L; Mullings, Anthony M

2008-01-01

195

The Survivor Unmet Needs Survey (SUNS) for haematological cancer survivors: a cross-sectional study assessing the relevance and psychometric properties  

PubMed Central

Background Relevant and psychometrically sound needs assessment tools are necessary for accurate assessment of haematological cancer survivors unmet needs. No previous study has developed nor psychometrically evaluated a comprehensive needs assessment tool for use with population-based samples of haematological cancer survivors. This study aimed to assess the validity and reliability of the Survivor Unmet Needs Survey (SUNS) with haematological cancer survivors. Methods The relevance, content and face validity of the SUNS to haematological cancer survivors was assessed using qualitative interviews. Psychometric evaluation was conducted using data collected from haematological cancer survivors, aged 18–80 years at recruitment and recruited from four Australian cancer registries. Construct, convergent and discriminant validity; internal reliability and floor and ceiling effects were assessed. A second survey was completed by a sub-sample of survivors recruited from two of the four registries to assess test-retest reliability. Results Results from 17 qualitative interviews confirmed the relevance, face and content validity of the original items of the SUNS for use with haematological cancer survivors. Overall, 1,957 eligible haematological cancer survivors were contacted by the cancer registries. Of these 1,280 were sent a survey, and 715 returned a survey (37% of eligible survivors contacted and 56% of survivors sent a survey). A total of 529 survivors completed all 89 items of the SUNS and were included in the exploratory factor analysis. Exploratory factor analysis supported the original five-factor structure of the SUNS. Evidence for convergent validity was established, with all five domains of the SUNS illustrating a moderate positive correlation with all three subscales of the Depression Anxiety and Stress Scale (DASS-21). All Cronbach’s alpha values were above 0.9 and all corrected item-total correlations were acceptable (>0.2). Criteria for discriminant validity was not met, with only 10 of the 15 (67%) a-priori hypotheses supported. Test-retest reliability was acceptable for 40 of the 89 items (45%) and for three of the five domains. Significant floor effects were evident for all five domains. Conclusions The SUNS demonstrates evidence for multiple features of validity and reliability as a measure of unmet needs for haematological cancer survivors. However, evidence supporting some psychometric properties was limited.

2014-01-01

196

Preliminary study of the fungal ecology at the haematology and medical-oncology ward in bamako, mali.  

PubMed

Data on fungal epidemiology in sub-Saharan African countries are scarce. This exploratory study aimed to characterize the fungal flora at the Onco-Haematology ward of the National Teaching Hospital of Point G in Bamako, Mali. A cross-sectional survey was conducted in the dry and in the rainy seasons. Nasal swab and sputum samples were collected from the hospitalized patients while airborne fungal spores were collected using electrostatic dust-fall collectors. Fungi were identified by their morphological characteristics and MALDI-TOF mass spectrometry. Candida albicans was the most frequent yeast species colonizing patients; Aspergillus species were isolated in 86 % of the patients and were the main airborne environmental contaminants. Overall, airborne fungal contamination rates increased from 33.8 % in the dry to 66.2 % in the rainy season (p < 0.001). The most frequent Aspergillus species were Aspergillus niger (36.6 %) and Aspergillus flavus (32.92 %). In contrast, Aspergillus fumigatus (5.43 %) was relatively rare. This high level of fungal exposure raises concern regarding the management of at-risk patients in this Onco-Haematology ward and stresses the need for strengthening the mycological diagnostic capacities to accompany the implementation of adapted fungal infection prevention and management policies. PMID:24889723

Niaré-Doumbo, Safiatou; Normand, Anne Cécile; Diallo, Yacouba Lazarre; Dembelé, Abdoul Karim; Thera, Mahamadou A; Diallo, Dapa; Piarroux, Renaud; Doumbo, Ogobara; Ranque, Stéphane

2014-08-01

197

Preventing invasive fungal disease in patients with haematological malignancies and the recipients of haematopoietic stem cell transplantation: practical aspects.  

PubMed

Invasive fungal disease (IFD), predominantly aspergillosis, is associated with significant morbidity and mortality in immunocompromised patients, especially those with haematological malignancies and recipients of allogeneic haematopoietic stem cell transplantation. There has been a great deal of scientific debate as to the effectiveness of antifungal prophylaxis in preventing infection in different patient groups and in which patients it is an appropriate management option. Deciding on an appropriate prophylaxis regimen for IFD is challenging as the incidence varies among different patient groups, due to the varied nature of their underlying haematological disease, and in different regions and centres. Attempts have been made to define risk factors and include them in treatment protocols. Impaired immune status of the patient, especially neutropenia, is a key risk factor for IFD and can sometimes be related to specific polymorphisms of genes controlling innate immunity. Risk factors also vary according to the type of fungal pathogen. Consequently, prophylaxis needs to be tailored to individual patient groups. Furthermore, the choice of antifungal agent for prophylaxis depends on the potential for drug-drug interactions with the patients' concomitant medications. Additional challenges are optimal timing of antifungal prophylaxis, when to change from prophylaxis to antifungal treatment and how to prevent recurrence of IFD. This article considers the use of antifungal prophylaxis for patients at risk of IFD in daily clinical practice, with clinical profiles that may be distinct from those covered by guidelines, and aims to provide practical advice for treatment of these patient groups. PMID:24155144

Akan, Hamdi; Antia, Vistasp P; Kouba, Michal; Sinkó, János; T?nase, Alina Daniela; Vrhovac, Radovan; Herbrecht, Raoul

2013-11-01

198

Biochemical and haematological evaluation of repeated dose exposure of male Wistar rats to an ethanolic extract of Artemisia annua.  

PubMed

Artemisia annua is widely used for the treatment of malaria and other disorders. In a previous study, the artemisinin concentration in the dry leaves of A. annua grown under humid tropical conditions was determined to be 1.098% using reversed phase high performance liquid chromatography. In the current study, biochemical and haematological evaluations of ethanolic leaf extracts derived from such plants (EAA) were carried out in 20 male Wistar rats. Rats were divided into four study groups of saline-treated (control) and test groups exposed orally to graded doses of EAA for 28 days. The results showed that the liver function and haematological indices, and testosterone levels were not adversely affected. High density lipoprotein -cholesterol was reduced at 100?mg/kg of EAA, atherogenic index as well as low density lipoprotein -cholesterol was raised, and glucose concentration was reduced significantly at the 100 and 200?mg/kg of EAA (p?

Eteng, Mbeh U; Abolaji, Amos O; Ebong, Patrick E; Brisibe, Ebiamadon Andi; Dar, Ahsana; Kabir, Nurul; Iqbal Choudhary, M

2013-04-01

199

Contribution of new diagnostic approaches to antifungal treatment plans in high-risk haematology patients.  

PubMed

In high-risk patient cohorts, such as patients after solid-organ or allogeneic stem-cell transplantation, or patients with acute leukaemia, early diagnosis of invasive fungal infections (IFIs) is essential, as delayed or missing diagnosis of IFI results in increasing rates of mortality. However, diagnosis of most IFIs, especially of invasive aspergillosis, is difficult because classic tests have low sensitivity and specificity, and radiology often provides non-specific and transient results. The limited sensitivity and specificity of conventional assays for the detection of IFI and the growing number of immunocompromised patients who are at risk for opportunistic fungal infections have led to the development of new assays. These methods include antigen detection systems, such as ELISAs, and different molecular methods (PCR assays). Serological tests, such as the detection of the carbohydrate galactomannan, are standardised and commercially available. However, they still need to be evaluated in large patient cohorts, especially children. The benefit of antibody detection remains unclear if patients are under immune suppression or are heavily colonised but not infected. A range of different PCR assays (conventional, nested, real-time) have been developed, targeting different gene regions (cytochrome P450, heat-shock proteins, 18S, 5.8S, 28S, internal transcribed spacer), including a variety of amplicon detection methods, such as gel electrophoresis, hybridisation with specific probes, ELISA and restriction fragment length polymorphism. These molecular assays provide high potential in terms of sensitivity and specificity, but vary widely in their feasibility and up to now have not been standardised. Taken together, new non-culture-based diagnostic assays are appropriate as simple and rapid screening tests with high sensitivities and quick turnaround times. Thus, they might help to reduce empirical antifungal therapy and might be valuable tools to allow early initiation and monitoring of pre-emptive antifungal therapy. In this review, we assess the performance of a variety of non-culture-based tests for the detection of IFI in high-risk haematological patients, with emphasis on the impact of the assays on different management strategies. PMID:18430128

Einsele, H; Loeffler, J

2008-05-01

200

Neonatal haematology in Zimbabwe. II: The red cell and white cell parameters.  

PubMed

Red cell and white cell parameters were determined in 600 (310 male and 290 female) normal Black Zimbabwean neonates with a mean +/- sd birth weight of 3.0 +/- 0.4 (range 2.04 to 4.50, median 3.0) kg using a Coulter Counter Model S Plus. Cord anaemia (cord Hb < 13.5 gdl-1) was detected in 60 (10 pc) of the neonates. Although the male babies were significantly heavier than the females (p = 0.004), there were no significant differences (p > 0.05) in the red cell and white cell indices between the two sexes. When the haematological parameters of the 540 (90 pc) non-anaemic (cord Hb > or = 13.5 gdl-1) neonates were analysed, the mean +/- sd values which may serve as local reference standards were: Hb 15.2 +/- 1.8 (range 13.5 to 19.4) gdl-1, Hct 47.3 4.9 (range 38.6 to 60.3) pc, MCV 107.8 +/- 9.4 (range 88.8 to 134.3) fl, MCH 31.9 +/- 3.4 (range 25.2 to 45.2) pg, MCHC 32.9 +/- 1.3 (range 30.0 to 38.3) gdl-1, nucleated red blood cells 6.1 +/- 5.9 (range 0 to 55) per 100 white blood cells, reticulocyte count 5.0 +/- 1.9 (range 0 to 24.0) pc, total leucocyte count 13.8 +/- 4.4 (range 4.6 to 132.8) x 10(9)l-1; differential count: neutrophils 7.30 +/- 2.90 (range 1.72 to 18.02) x 10(9) l-1, lymphocytes 5.67 +/- 2.47 (range 0.98 to 16.14) x 10(9) l-1, monocytes 0.81 +/- 0.83 (range 0 to 5.58) x 10(9) l-1, 1 and eosinophils 0.08 +/- 0.13 (range 0 to 0.72) x 10(9)l-1.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:7788674

Mukiibi, J M; Nkrumah, F K; Kaur, M; Pollard, R; Akino, V; Nhembe, M

1995-03-01

201

Effects of Trypanosoma congolense and nutritional supplements in Djallonké ewes on live weight during pregnancy, post partum weight, haematology parameters and lamb performance  

Microsoft Academic Search

The effects of Trypanosoma congolense infection and nutritional supplements on live weight changes during pregnancy, haematology traits and offspring performance were studied in 42 Djallonké ewes. A randomised block design was used to allocate ewes to four treatment combinations, of which two were on a restricted diet (L) and the remainder on an unrestricted diet (H). Half of each nutritional

I. Jeffcoate; P. Holmes

1998-01-01

202

Effect of Ramadan fasting on some biochemical and haematological parameters in Tunisian youth soccer players undertaking their usual training and competition schedule  

Microsoft Academic Search

Competitive Muslim athletes often have to train and compete during the holy month of Ramadan when they abstain from food and drink from sunrise to sunset. In this study, we investigated the effect of Ramadan fasting on some biochemical and haematological parameters in 78 Tunisian junior male soccer players aged 16–19 years who continued their usual schedule of daily training

Ronald J. Maughan; John B. Leiper; Zakia Bartagi; Rym Zrifi; Yacine Zerguini; Jiri Dvorak

2008-01-01

203

Homozygous PMS2 germline mutations in two families with early-onset haematological malignancy, brain tumours, HNPCC-associated tumours, and signs of neurofibromatosis type 1  

Microsoft Academic Search

Heterozygous germline mutations in mismatch repair (MMR) genes MLH1, PMS2, MSH2, and MSH6 cause Lynch syndrome. New studies have indicated that biallelic mutations lead to a distinctive syndrome, childhood cancer syndrome (CCS), with haematological malignancies and tumours of brain and bowel early in childhood, often associated with signs of neurofibromatosis type 1. We provide further evidence for CCS reporting on

Stefan Krüger; Miriam Kinzel; Constanze Walldorf; Sven Gottschling; Andrea Bier; Sigrid Tinschert; Arend von Stackelberg; Wolfram Henn; Heike Görgens; Stephanie Boue; Konrad Kölble; Reinhard Büttner; Hans K Schackert

2008-01-01

204

A comparison of the effect of bleeding site on haematological and plasma chemistry values of F344 rats: The inferior vena cava, abdominal aorta and orbital venous plexus  

Microsoft Academic Search

Of paramount importance to most toxicity studies in rats is the evaluation of haematological, coagulation and clinical chemistry parameters. In European and North American countries, the orbital venous plexus (OVP) is currently the most common route for obtaining blood, whereas in Japan the inferior vena cava (IVC) and abdominal aorta (AA) are the preferred routes. In order to compare clinical

T. Matsuzawa; H. Tabata; M. Sakazume; S. Yoshida; S. Nakamura

1994-01-01

205

The Effects of Pollution on Haematological Parameters of Black Goby (Gobius niger L., 1758) in Foça and Alia?a Bays  

Microsoft Academic Search

In this study, the haematological parameters (Red blood cells, haemoglobin, mean celllular haemoglobin content, mean cellular volume, mean cellular haemoglobin concentration, platelet) of Gobius niger collected from several selected stations in Foça and Alia?a Bays, where a petroleum refinery is located, have been measured. The statistically important differences were found between the measured red blood cells haemoglobin, haematocrit, mean cell

Selma Katalay; Hatice Parlak

206

Haematological and serum biochemical changes in pigs administered with ascorbic acid and transported by road for four hours during the harmattan season  

Microsoft Academic Search

This study was conducted with the aim of investigating the effect of ascorbic acid (AA) on haematology and serum biochemical responses of pigs transported for short journey (4 h) during the harmattan season. Sixteen pigs administered with AA at a dose of 250 mg\\/kg per os and individually served as experimental animals, and 13 others each administered orally with sterile

A. Y. Adenkola; J. O. Ayo; A. K. B. Sackey; A. B. Adelaiye

207

Mastocytaemia in cats: prevalence, detection and quantification methods, haematological associations and potential implications in 30 cats with mast cell tumours.  

PubMed

The significance of mastocytaemia in cats is different from that in dogs because it appears exclusively associated with mast cell neoplasia. The prevalence of mastocytaemia was 0.05% of all feline submissions to a private laboratory and 43% in cats with mast cell neoplasia. None of 30 healthy cats had mastocytaemia. There was no sex bias or significant age difference between mastocytaemic and non-mastocytaemic cats with mast cell tumours (MCT). Buffy coat (BC) examination was the best screening method for detection of mastocytaemia but direct blood film examination was more accurate for quantifying degree of mastocytaemia. BC examination should be performed in all cases of suspected/known mast cell neoplasia as mastocytaemia was missed on nearly 30% of occasions when direct film examination only was used. Mastocytaemia was associated with decreased haematocrit (HCT) but not with other haematological parameters. Mastocytaemic cats can survive significant lengths of time (up to 27 months) even when splenectomy is not performed. PMID:21050784

Skeldon, Nicola C A; Gerber, Karen L; Wilson, Randy J; Cunnington, Sophie J

2010-12-01

208

Haematological alterations induced by oral subacute exposure to fenvalerate, nitrate and their combination in domestic buffalo, Bubalus bubalis.  

PubMed

The present study investigated haematological alterations induced by oral subacute exposure to fenvalerate, nitrate and their combination in the domestic buffalo, Bubalus bubalis. Fenvalerate exposure produced significant declines in haemoglobin (Hb), total leukocyte count (TLC), total erythrocyte count (TEC) and mean corpuscular haemoglobin concentration (MCHC), and a corresponding elevation in mean corpuscular volume (MCV). Following oral exposure to sodium nitrate, significant declines in blood Hb, TLC, TEC, MCH and MCHC, and a significant elevation in MCV occurred. Combined exposure to fenvalerate and sodium nitrate produced severe effects with an appreciably more prominent decline in Hb, TLC, TEC and MCHC and a significant elevation in MCV. The percentage of methaemoglobin was observed to follow an elevating trend in animals exposed to sodium nitrate alone (0.69 %-13.8 %) and in combination with fenvalerate (0.75 %-13.7 %). PMID:24687223

Gill, Kamalpreet Kaur; Sandhu, H S; Kaur, Rajdeep

2014-06-01

209

Growth performance, haematology and serum biochemistry of African catfish (Clarias gariepinus) fingerlings fed graded levels of dietary fumonisin B1.  

PubMed

Fingerlings of Clarias gariepinus were used to evaluate the effect of dietary fumonisin B1 (FB1), a mycotoxin produced by Fusarium verticillioides, on growth, haematological and serum biochemical parameters. The fingerlings were sorted, weighed and randomly stocked in 16 plastic tanks at the rate of 20 fingerlings per tank. Fusarium-cultured maize grains containing FB1 were used to formulate three diets containing approximately 5.0, 10.0 and 15.0 mg FB1/kg, constituting diets 2, 3, and 4 respectively. These three diets, plus diet 1, which contained non-Fusarium cultured maize grains that served as the control, were used in a 6-week feeding trial. The final weight gains by the fingerlings were significantly (P?haematological parameters and serum protein constituents in the fingerlings. These may have a significant impact on physiological activities and may be vital in immunosuppression in the fingerlings with a strong negative impact on subsequent performance of the fish. PMID:23605483

Gbore, Francis A; Adewole, Adeyemo M; Oginni, Olatunde; Oguntolu, Mercy F; Bada, Ayodeji M; Akele, Olatunbosun

2010-11-01

210

Blood Haematology, Serum Thyroid Hormones and Glutathione Peroxidase Status in Kacang Goats Fed Inorganic Iodine and Selenium Supplemented Diets  

PubMed Central

The effects of dietary supplementation of selenium (Se), iodine (I), and a combination of both on the blood haematology, serum free thyroxine (FT4) and free triiodothyronine (FT3) hormones and glutathione peroxidase enzyme (GSH-Px) activity were examined on twenty four (7 to 8 months old, 22±1.17 kg live weight) Kacang crossbred male goats. Animals were randomly assigned to four dietary treatments (6 animals in each group). Throughout 100 d of feeding trial, the animals of control group (CON) received a basal diet, while the other three groups were offered basal diet supplemented with 0.6 mg/kg diet DM Se (SS), or 0.6 mg/kg diet DM I (PI), or a combination of both Se and I, each at 0.6 mg/kg diet DM (SSPI). The haematological attributes which are haemoglobin (Hb), red blood cell (RBC), packed cell volume (PCV), mean cell volume (MCV), white blood cells (WBC), band neutrophils (B Neut), segmented neutrophils (S Neut), lymphocytes (Lymph), monocytes (Mono), eosinophils (Eosin) and basophils (Baso) were similar among the four treatment groups, while serum levels of Se and I increased significantly (p<0.05) in the supplemented groups. The combined dietary supplementation of Se and I (SSPI) significantly increased serum FT3 in the supplemented animals. Serum GSH-Px activity increased significantly in the animals of SS and SSPI groups. It is concluded that the dietary supplementation of inorganic Se and I at a level of 0.6 mg/kg DM increased serum Se and I concentration, FT3 hormone and GSH-Px activity of Kacang crossbred male goats.

Aghwan, Z. A.; Sazili, A. Q.; Alimon, A. R.; Goh, Y. M.; Hilmi, M.

2013-01-01

211

Effect of Maternal HIV-1 Status and Antiretroviral Drugs on Haematological Profiles of South African Infants in Early Life  

PubMed Central

Maternal HIV-1 status and antiretroviral drug exposure may influence the haematological profiles of infants. We recruited infants from 118 uninfected control women and from 483 HIV-1 infected women who received no antiretroviral drugs (n=28), or received single-dose Nevirapine (sdNVP) (n=424) or triple-drug combination therapy (n=31) to reduce HIV-1 transmission. Blood was drawn from infants within 24 hours of delivery or 6-12 weeks post-delivery and full blood counts performed using a fully automated AcT-5-diff haematology analyser and reference controls. Exposed uninfected (EU; no NVP) differed from control infants only in having lower basophil counts and percentages. In all infant groups, leukocyte profiles showed characteristic quantitative changes with age in the first 6 weeks of life. HIV-1 infected infants displayed by 6 weeks elevations in white blood cells, lymphocyte, monocyte and basophil counts, and monocyte and basophil percentages, when compared to EU infants. At birth EU NVP-treated infants exhibited elevated monocyte percentages and counts and basophil counts that did not persist at 6 weeks. Interestingly, EU newborns of mothers with high CD4 counts (> 500 cells/?l) that had taken sdNVP had significantly elevated white blood cell, monocyte and basophil counts when compared to newborn infants of mothers with similar CD4 counts that had not taken sdNVP; this was not evident in infants of mothers with CD4 counts <200 cells/?l. These previously undescribed features may affect immune response capability in early life and clinical consequences of such changes need to be further investigated.

Schramm, Diana B; Anthony, Fiona; Mathebula, Busani; Sherman, Gayle; Coovadia, Ashraf; Gray, Glenda E; Kuhn, Louise; Tiemessen, Caroline T

2010-01-01

212

Visualising the cross-level relationships between pathological and physiological processes and gene expression: analyses of haematological diseases.  

PubMed

The understanding of pathological processes is based on the comparison between physiological and pathological conditions, and transcriptomic analysis has been extensively applied to various diseases for this purpose. However, the way in which the transcriptomic data of pathological cells relate to the transcriptomes of normal cellular counterparts has not been fully explored, and may provide new and unbiased insights into the mechanisms of these diseases. To achieve this, it is necessary to develop a method to simultaneously analyse components across different levels, namely genes, normal cells, and diseases. Here we propose a multidimensional method that visualises the cross-level relationships between these components at three different levels based on transcriptomic data of physiological and pathological processes, by adapting Canonical Correspondence Analysis, which was developed in ecology and sociology, to microarray data (CCA on Microarray data, CCAM). Using CCAM, we have analysed transcriptomes of haematological disorders and those of normal haematopoietic cell differentiation. First, by analysing leukaemia data, CCAM successfully visualised known relationships between leukaemia subtypes and cellular differentiation, and their characteristic genes, which confirmed the relevance of CCAM. Next, by analysing transcriptomes of myelodysplastic syndromes (MDS), we have shown that CCAM was effective in both generating and testing hypotheses. CCAM showed that among MDS patients, high-risk patients had transcriptomes that were more similar to those of both haematopoietic stem cells (HSC) and megakaryocyte-erythroid progenitors (MEP) than low-risk patients, and provided a prognostic model. Collectively, CCAM reveals hidden relationships between pathological and physiological processes and gene expression, providing meaningful clinical insights into haematological diseases, and these could not be revealed by other univariate and multivariate methods. Furthermore, CCAM was effective in identifying candidate genes that are correlated with cellular phenotypes of interest. We expect that CCAM will benefit a wide range of medical fields. PMID:23301083

Ono, Masahiro; Tanaka, Reiko J; Kano, Manabu; Sugiman, Toshio

2013-01-01

213

Description of outcomes of experimental infection with feline haemoplasmas: Copy numbers, haematology, Coombs' testing and blood glucose concentrations  

PubMed Central

The aim of this study was to compare blood copy, haematological and glucose values between cats experimentally infected with either Mycoplasma haemofelis (Group HF: 10 cats), ‘Candidatus M. haemominutum’ (Group HM: 3 cats) or ‘Candidatus M. turicensis’ (Group TU: 3 cats). Blood samples were collected regularly up to 85 days post-infection (DPI) for haemoplasma real-time quantitative PCR, haematology, Coombs’ testing and blood glucose measurement. Statistical analysis was performed using a general linear model (ANOVA) appropriate for a repeated measures experiment with significance set as P < 0.05. Cats in Group TU had significantly lower blood copy numbers than cats in Group HF (P < 0.001) and HM (P < 0.001). All Group HF cats developed anaemia (often severe), macrocytosis and evidence of erythrocyte-bound antibodies whereas Groups HM and TU cats did not. Group HF had significantly lower PCVs, haemoglobin concentrations and red blood cell counts, and significantly higher mean cell volumes, than Groups HM and TU. In Group HF, erythrocyte-bound antibodies reactive at 4 °C (both IgM and IgG) appeared between 8 and 22 DPI and persisted for two to four weeks, whereas those reactive at 37 °C (primarily IgG) appeared between 22 and 29 DPI and persisted for one to five weeks. In most cats antibodies appeared after the fall in haemoglobin started. Although Group TU had significantly lower glucose concentrations than Groups HF (P = 0.006) and HM (P = 0.027), mean blood glucose concentrations remained within the reference range in all groups. This study demonstrates that M. haemofelis infection, in contrast to ‘Candidatus M. haemominutum’ and ‘Candidatus M. turicensis’ infection, can result in a severe macrocytic anaemia and the development of cold and warm reactive erythrocyte-bound antibodies.

Tasker, Severine; Peters, Iain R.; Papasouliotis, Kostas; Cue, Simon M.; Willi, Barbara; Hofmann-Lehmann, Regina; Gruffydd-Jones, Timothy J.; Knowles, Toby G.; Day, Michael J.; Helps, Chris R.

2009-01-01

214

Ascorbate2-sulfate as a dietary vitamin C source for atlantic salmon ( Salmo salar ): 1. Growth, bioactivity, haematology and humoral immune response  

Microsoft Academic Search

The present experiment shows that ascorbate-2-sulfate (AS) is not equivalent to ascorbic acid (AA) as a dietary vitamin C\\u000a source for Atlantic salmon (Salmo salar). Within reasonable feed supplemental levels AS does not provide the tissues with adequate supplies of vitamin C to secure\\u000a optimal physiological functions as demonstrated by biochemical and haematological analyses.\\u000a \\u000a AS could not be detected in

K. Sandnes; T. Hansen; J. E. A. Killie; R. Waagbø

1990-01-01

215

Changes in menstrual and haematological indices among norplant® acceptors 1 1 Norplant is a registered trademark of the Population Council for levonorgestrel subdermal implants  

Microsoft Academic Search

A longitudinal study of women using Norplant® was conducted to determine the effects of levonorgestrel implants on menstrual and haematological indices among the acceptors. Packed cell volume (PCV), white blood cell (WBC) concentrations (total and differential), and platelet concentration were performed at pre-insertion and at the 12-month follow-up. Each acceptor received a menstrual calendar to chart all bleeding, and spotting

A. O Aisien; A. S Sagay; G. E Imade; I. A. O Ujah; O. U Nnana

2000-01-01

216

Some haematological changes in hybrid catfish (Heterobranchus longifilis x Clarias gariepinus) fed different dietary levels of raw and boiled jackbean (Canavalia ensiformis) seed meal  

Microsoft Academic Search

Changes were observed in some haematological parameters of juvenile hybrid catfish (Heterobranchus longifilis x Clarias gariepinus) fed raw and 60 min-boiled jackbean seed meal (JBSM) at different dietary levels for 56 days. The haematocrit (packed cell volume, PCV), red blood cell (RBC) count, white blood cell (WBC) count and haemoglobin (Hb) concentration decreased significantly (P<0.05) with increasing dietary JBSM level.

D. I. Osuigwe; A. I. Obiekezie; G. C. Onuoha

217

Influence of esterified-glucomannan on performance and organ morphology, serum biochemistry and haematology in broilers exposed to individual and combined mycotoxicosis (aflatoxin, ochratoxin and T-2 toxin)  

Microsoft Academic Search

1. A study was conducted to evaluate the individual and combined effects of aflatoxin B 1 (AF), ochratoxin A (OA) and T-2 toxin (T-2) on performance, organ morphology, serum biochemistry and haematology of broiler chickens and the efficacy of esterified-glucomannan (E-GM), a cell wall derivative of Saccharomyces cerevisiae 1026 in their counteraction. 2. Two dietary inclusion rates of AF (0

M. V. L. N. Raju; G. Devegowda

2000-01-01

218

Influence of ?-1,3\\/1,6 glucan applications on some non-specific cellular immune response and haematologic parameters of healthy Nile tilapia (Oreochromis niloticus L., 1758)  

Microsoft Academic Search

In this study, the effects of ?-glucan administration at different dosages on non-specific immune response and haematological parameters in healthy Nile tilapia (Oreochromis niloticus L.) were investigated. Fish were fed with diets containing 0.5% and 0.1% glucan (experiment) and without glucan (control) for 2 weeks. After feeding for 2 weeks with 2 different dosages, erythrocyte (RBC), leukocyte (WBC), hematocrit (Hct),

Selçuk DUMAN

2010-01-01

219

Effects of Saccharomyces cerevisiae extract on haematological parameters, immune function and the antioxidant defence system in breeder hens fed aflatoxin contaminated diets  

Microsoft Academic Search

1. The study was conducted to investigate the efficacy of Saccharomyces cerevisiae extract (SC) on haematological parameters, immune function, and the antioxidant defence system in breeder hens fed a diet contaminated with low level aflatoxin (AF).2. Forty-eight Ross 308 breeder hens were fed on diets containing AF (0 or 100?µg\\/kg) and SC (0 or 1?g\\/kg) in a 2?×?2 factorial arrangement.

E. Matur; E. Ergul; I. Akyazi; E. Eraslan; G. Inal; S. Bilgic; H. Demircan

2011-01-01

220

Comparison of conventional and organic management conditions on growth performance, carcass characteristics and haematological parameters in Karacabey Merino and Kivircik breeds  

Microsoft Academic Search

Growth performance, carcass characteristics, post-slaughtering and haematological parameters of Kivircik and Karacabey Merino\\u000a male lambs in conventional and organic management systems were compared. The animals which were weaned at 7 weeks of age were\\u000a divided into Kivircik conventional, Kivircik organic (KO), Karacabey Merino conventional and Karacabey Merino organic (MO)\\u000a groups containing 12 lambs each. Fattening was ended when lambs attained 35 kg

Deniz Soysal; Recep Cibik; Cenk Aydin; ?brahim Ak

2011-01-01

221

Selected Haematological and Biochemical Parameters of Blood in Rats After Subchronic Administration of Vanadium and\\/or Magnesium in Drinking Water  

Microsoft Academic Search

The purpose of these studies was to evaluate the effect of selected vanadium and magnesium doses on certain haematological\\u000a and biochemical blood parameters in rats. Outbred 2-month-old, albino male Wistar rats received for a period of 6 weeks, as\\u000a a sole drinking liquid, the following water solutions: group II, sodium metavanadate (SMV) at a concentration of 0.125 mg\\u000a V\\/mL; group

Agnieszka ?cibior; Halina Zaporowska; Jaros?aw Ostrowski

2006-01-01

222

Effects of continuous supplementations of ascorbic acid, aspirin, vitamin E and selenium on some haematological parameters and serum superoxide dismutase level in broiler chickens  

Microsoft Academic Search

1. This study was conducted using male broiler chickens to determine the effects of ascorbic acid, aspirin, ascorbic acid+aspirin, vitamin E+selenium and ascorbic acid+aspirin+vitamin E+selenium supplementations on haematological parameters and serum superoxide dismutase concentration. 2. One hundred and twenty day-old male Hubbunt broiler chicks were randomly divided into 6 experimental groups of 20 chicks each and placed in different pens.

B. Tras; F. Inal; A. L. Bas; V. Altunok; M. Elmas; E. Yazar

2000-01-01

223

Physiological responses to starvation in the European eel ( Anguilla anguilla ): effects on haematological, biochemical, non-specific immune parameters and skin structures  

Microsoft Academic Search

The physiological effects of short-term starvation on some haematological, biochemical and non-specific immune response parameters\\u000a together with the histological structure of the skin, were investigated in the European eel, Anguilla anguilla. Blood haemoglobin and haematocrit, serum glucose and cortisol, hemolysins, haemagglutinins, and lysozyme in the plasma,\\u000a kidney and epidermal extract, were measured in fish after 31, 42 and 58 days of starvation,

G. Caruso; G. Maricchiolo; V. Micale; L. Genovese; R. Caruso; M. G. Denaro

2010-01-01

224

Pathological, biochemical and haematological investigations on the protective effect of alpha-lipoic acid in experimental aflatoxin toxicosis in chicks.  

PubMed

1. The purpose of this study was to investigate the protective effect of alpha-lipoic acid (LA) on aflatoxin (AF) toxicosis in chicks. 2. Groups of 10 Ross PM3 chicks were given, for 21 d, no AF (C), 60 mg/kg/bwt of alpha-lipoic acid (LA), 150 ppb of aflatoxin (AF1), 150 ppb of aflatoxin plus 60 mg/kg/bwt of alpha-lipoic acid (AF1 + LA), 300 ppb of aflatoxin (AF2), and 300 ppb of aflatoxin plus 60 mg/kg/bwt of alpha-lipoic acid (AF2 + LA). Before the animals were killed, blood samples were drawn for haematological analysis, and then tissue samples were collected for histopathological investigation. Immunohistochemical staining was performed against inducible nitric oxide synthase (iNOS) and nitrotyrosine on liver samples. Apoptotic cell death in liver was assessed by in situ TUNEL assay. The malondialdehyde (MDA) and reduced glutathione (GSH) concentrations in liver and kidney were also determined. 3. Hydropic degeneration and occasional necrosis, bile duct hyperplasia and periportal fibrosis were observed in the livers of AF-treated groups. The severity of these changes was reduced in LA-supplemented AF groups. Occasionally, thymic cortical atrophy, lymphoid depletion in spleen and bursa of Fabricius, and degeneration in the kidney tubule epitheliums were detected in AF groups. The severity of these degenerative changes was slightly reduced in LA supplemented groups. 4. There was moderate to strong iNOS and nitrotyrosine immunoreactivity in the livers of AF groups, while decreased immunoreactivity was observed against both antibodies in the LA supplemented groups. Apoptotic cells were numerous in the AF groups, while greatly reduced in LA supplemented groups. 5. In the liver and kidney of AF-treated groups given 300 ppb of aflatoxin, MDA concentrations were increased as GSH decreased, compared to the control group. LA supplementation of AF-treated birds improved the results compared to the AF only groups, however a statistical difference was observed only in liver tissues between AF2 + LA and AF2 groups. Haematological variables showed no differences among the groups. 6. In conclusion, supplementation of feed with the antioxidant LA, might ameliorate the degenerative effects caused by aflatoxin due to lipid peroxidation. PMID:20390578

Karaman, M; Ozen, H; Tuzcu, M; Ci?remi?, Y; Onder, F; Ozcan, K

2010-02-01

225

Loci Associated with N-Glycosylation of Human Immunoglobulin G Show Pleiotropy with Autoimmune Diseases and Haematological Cancers  

PubMed Central

Glycosylation of immunoglobulin G (IgG) influences IgG effector function by modulating binding to Fc receptors. To identify genetic loci associated with IgG glycosylation, we quantitated N-linked IgG glycans using two approaches. After isolating IgG from human plasma, we performed 77 quantitative measurements of N-glycosylation using ultra-performance liquid chromatography (UPLC) in 2,247 individuals from four European discovery populations. In parallel, we measured IgG N-glycans using MALDI-TOF mass spectrometry (MS) in a replication cohort of 1,848 Europeans. Meta-analysis of genome-wide association study (GWAS) results identified 9 genome-wide significant loci (P<2.27×10?9) in the discovery analysis and two of the same loci (B4GALT1 and MGAT3) in the replication cohort. Four loci contained genes encoding glycosyltransferases (ST6GAL1, B4GALT1, FUT8, and MGAT3), while the remaining 5 contained genes that have not been previously implicated in protein glycosylation (IKZF1, IL6ST-ANKRD55, ABCF2-SMARCD3, SUV420H1, and SMARCB1-DERL3). However, most of them have been strongly associated with autoimmune and inflammatory conditions (e.g., systemic lupus erythematosus, rheumatoid arthritis, ulcerative colitis, Crohn's disease, diabetes type 1, multiple sclerosis, Graves' disease, celiac disease, nodular sclerosis) and/or haematological cancers (acute lymphoblastic leukaemia, Hodgkin lymphoma, and multiple myeloma). Follow-up functional experiments in haplodeficient Ikzf1 knock-out mice showed the same general pattern of changes in IgG glycosylation as identified in the meta-analysis. As IKZF1 was associated with multiple IgG N-glycan traits, we explored biomarker potential of affected N-glycans in 101 cases with SLE and 183 matched controls and demonstrated substantial discriminative power in a ROC-curve analysis (area under the curve?=?0.842). Our study shows that it is possible to identify new loci that control glycosylation of a single plasma protein using GWAS. The results may also provide an explanation for the reported pleiotropy and antagonistic effects of loci involved in autoimmune diseases and haematological cancer.

Muzinic, Ana; Novokmet, Mislav; Polasek, Ozren; Gornik, Olga; Kristic, Jasminka; Keser, Toma; Vitart, Veronique; Scheijen, Blanca; Uh, Hae-Won; Molokhia, Mariam; Patrick, Alan Leslie; McKeigue, Paul; Kolcic, Ivana; Lukic, Ivan Kresimir; Swann, Olivia; van Leeuwen, Frank N.; Ruhaak, L. Renee; Houwing-Duistermaat, Jeanine J.; Slagboom, P. Eline; Beekman, Marian; de Craen, Anton J. M.; Deelder, Andre M.; Zeng, Qiang; Wang, Wei; Hastie, Nicholas D.; Gyllensten, Ulf; Wilson, James F.; Wuhrer, Manfred; Wright, Alan F.; Rudan, Igor

2013-01-01

226

Effects of urea-treated fibrous diets on nutrient intake, digestibility, performance and haematological parameters of Yankasa rams.  

PubMed

An experiment was conducted to investigate the effects of urea-treated fibrous diets on the intake, nutrient digestibility, performance and haematological parameters of Yankasa rams. A total of 48 Yankasa rams (BW 10.00 ± 1.50 kg; 6-8 months old) were allocated into four treatment groups in a completely randomised design (12 rams per treatment). Animals were placed on complete rations of yam peels, maize bran and rice husk treated with 0, 1.0, 1.5 or 2.0% urea. The experiment lasted for 18 weeks. Yankasa rams fed with urea-treated diets had higher feed intake (949.88 and 938.04 g/day for U?? and U??, respectively), daily weight gain (227.67 and 181.00 g/day for U?? and U??, respectively) and better feed conversion ratio (4.17 and 5.18 for U?? and U??, respectively). Rams on urea-treated diets had higher haemoglobin and red blood cell contents and higher weight gains, indicating that urea treatment enhanced nutrient supply and utilisation at the tissue level. It was concluded that urea treatment of fibrous farm by-products is a promising feeding strategy especially during the dry season when there is scarcity of high-quality forages. Addition of 1.5% urea to roughage diets and farm by-products to form a total mixed ration may preclude the search for supplements. PMID:23397549

Alabi, John Olushola; Arigbede, Moses; Ng'ambi, Jones; Norris, David; Shiawoya, Emma; Onyekachi, Sunday

2013-08-01

227

The Effect of Mycotoxin Deoxynivalenol on Haematological and Biochemical Indicators and Histopathological Changes in Rainbow Trout (Oncorhynchus mykiss)  

PubMed Central

Deoxynivalenol (DON), produced by the Fusarium genus, is a major contaminant of cereal grains used in the production of fish feed. The effect of mycotoxin deoxynivalenol on rainbow trout (Oncorhynchus mykiss) was studied using a commercial feed with the addition of DON in a dose of 2?mg/kg feed. The fish (n = 40) were exposed to the mycotoxin for 23 days. The trout were divided into two groups, control and experimental groups. Control groups were fed a commercial feed naturally contaminated with a low concentration of DON (225??g/kg feed); experimental groups were fed a commercial feed with the addition of DON (1964??g/kg feed). Plasma biochemical and haematological indices, biometric parameters, and histopathological changes were assessed at the end of the experiment. The experimental groups showed significantly lower values in MCH (P < 0.05). In biochemical indices, after 23-day exposure, a significant decrease in glucose, cholesterol (P < 0.05), and ammonia (P < 0.01) was recorded in the experimental group compared to the control group. Our assessment showed no significant changes in biometric parameters. The histopathological examination revealed disorders in the caudal kidney of the exposed fish. The obtained data show the sensitivity of rainbow trout (O. mykiss) to deoxynivalenol.

Modra, Helena; Blahova, Jana; Franc, Ales; Fictum, Petr; Sevcikova, Marie; Svobodova, Zdenka

2014-01-01

228

Comparison of the clinical, microbiological, radiological and haematological features of foals with pneumonia caused by Rhodococcus equi and other bacteria.  

PubMed

The purpose of this study was to compare the clinical, microbiological, radiological, haematological and cytological features of foals with pneumonia caused by Rhodococcus equi infection and with other bacteria, in order to provide markers for early diagnosis and treatment. A retrospective study of 113 cases of bacterial pneumonia was undertaken. Although there was considerable overlap in the affected populations, foals with R. equi pneumonia were significantly younger and had higher respiratory rates. Radiological evidence of thoracic abscessation had a sensitivity of 71% and a specificity of 85% for the diagnosis of R. equi pneumonia. Foals positive for R. equi also had higher peripheral white cell counts and fibrinogen concentrations than animals not infected with this pathogen. Respiratory rate, fibrinogen concentration and the log of the neutrophil count were retained in the final multivariate analysis. Using microbiological culture as the 'gold standard', identification of Gram-positive coccobacilli in tracheal aspirates was highly specific (91%), but poorly sensitive (35%) for R. equi infection. White cell counts >20,000cells/?L (86% specificity), fibrinogen concentrations >700mg/dL (92% specificity), radiological evidence of thoracic abscessation (85% specificity), and the presence of Gram-positive coccobacilli in tracheal aspirates (91% specificity) in pneumonic foals are highly suggestive of R. equi infection and justify early targeted antimicrobial intervention while awaiting culture results. PMID:19932977

Leclere, Mathilde; Magdesian, K Gary; Kass, Philip H; Pusterla, Nicola; Rhodes, Diane M

2011-01-01

229

Haematopoietic stem cell transplantation for Diamond Blackfan anaemia: a report from the Italian Association of Paediatric Haematology and Oncology Registry.  

PubMed

Allogeneic haematopoietic stem cell transplantation (HSCT) is the only curative option for patients with Diamond Blackfan anaemia (DBA). We report the transplantation outcome of 30 Italian DBA patients referred to the Italian Association of Paediatric Haematology and Oncology Registry between 1990 and 2012. This is one of the largest national registry cohorts of transplanted DBA patients. Most patients (83%) were allografted after 2000. A matched sibling donor was employed in 16 patients (53%), the remaining 14 patients (47%) were transplanted from matched unrelated donors. Twenty-eight of the 30 patients engrafted. One patient died at day +6 due to veno-occlusive disease without achieving neutrophil recovery and another patient remained transfusion-dependent despite the presence of a full donor chimerism. The 5-year overall survival and transplant-related mortality was 74·4% and 25·6%, respectively. Patients younger than 10 years as well as those transplanted after 2000 showed a significantly higher overall survival and a significantly lower risk of transplant-related mortality. No difference between donor type was observed. Our data suggest that allogeneic HSCT from a related or unrelated donor was a reasonable alternative to transfusion therapy in young and well chelated DBA patients. PMID:24611452

Fagioli, Franca; Quarello, Paola; Zecca, Marco; Lanino, Edoardo; Corti, Paola; Favre, Claudio; Ripaldi, Mimmo; Ramenghi, Ugo; Locatelli, Franco; Prete, Arcangelo

2014-06-01

230

Experimental infection of chickens with an australian strain of reticuloendotheliosis virus. I. Clinical, pathological and haematological effects.  

PubMed

A wide range of clinical, pathological and haematological effects were found over a 40-week period in chickens inoculated at 1-day-old with a low-passage, cell-culture preparation of an Australian strain of reticuloendotheliosis virus. Feathering defects and statistically significant depression of body weights occurred in chickens up to 8 weeks of age. Other findings in birds that died or were culled during the 40-week experimental period included mild anaemia, leucopenia, heterophilia, hypoplasia of immune system organs, inflammation in visceral and nervous system organs, and bacterial or fungal infections. These results suggested that ill-thrift and death in some chickens infected with reticuloendotheliosis virus may be due to secondary infections with microorganisms subsequent to damage of immune system organs by that virus. Lymphoreticular-cell tumours of the liver, kidney or spleen were found in two birds aged 22 and 24 weeks. These results establish reticuloendotheliosis virus as a possible cause of tumours in adult fowls. Horizontal transmission of virus was demonstrated but the only abnormalities detected in the in-contact chickens were feathering defects. PMID:18770427

Grimes, T M; Bagust, T J; Dimmock, C K

1979-01-01

231

Effect of Aqueous Stem Bark Extract of Khaya senegalensis on Some Biochemical, Haematological, and Histopathological Parameters of Rats  

PubMed Central

The subchronic effect of aqueous stem bark extract of Khaya senegalensis on some biochemical, haematological, and histopathological parameters of rats was investigated. The rats were divided into six groups of five rats per group. Groups I to VI were administered graded doses of 0, 400, 800, 1200, 1600, and 2000?mg/kg?bw, respectively. The result of study revealed that administration of the Khaya senegalensis for twenty-eight days at the experimental dose resulted in significant (P < 0.05) increase in urea, electrolytes (Na+, K+), and creatinine levels. The extract also significantly (P < 0.05) increased serum activity of ALT, AST, and ALP. The levels of protein, albumin, and bilirubin were significantly changed when compared to their control values, but they were not dose dependent. The hematological indices assayed in this study were not significantly affected at the experimental dose when compared to the control values. Histological studies of the liver showed cellular degeneration and necrosis and bile duct hyperplasia and fibrosis with lymphocytic infiltration of the hepatocyte, providing supportive evidence for discussing the biochemical findings, indicative of functional derangement. The histological architecture of the kidney and that of the heart were however preserved. The result of this study indicates that the aqueous stem bark extract of K. senegalensis may affect the cellular integrity of vital organs of the body.

Onu, A.; Saidu, Y.; Ladan, M. J.; Bilbis, L. S.; Aliero, A. A.; Sahabi, S. M.

2013-01-01

232

Effects of growth hormone on growth performance, haematology, metabolites and hormones in iron-deficient veal calves.  

PubMed

Effects of subcutaneous (s.c.) administration of 50 micrograms/kg body weight of recombinant bovine growth hormone (rbGH) or saline were studied for 11 weeks in 40 intact male veal calves supplied 50 mg or 10 mg of iron (Fe)/kg of milk replacer (MR). Feed intake, average daily gain and growth: feed ratio were reduced in Fe-deficient calves, but not significantly influenced by rbGH. Plasma Fe and haemoglobin concentration, red-cell number and packed cell volume were decreased in Fe-deficient calves (P < 0.05) and rbGH further reduced red-cell number in Fe-deficient calves (P < 0.05). The age-dependent increase of total Fe binding capacity was greater in Fe-deficient calves and enhanced by rbGH (P < 0.05). Plasma urea concentrations increased, whereas glucose (G) and triiodothyronine (T3) levels decreased in Fe-deficient calves. rbGH significantly increased G in calves fed MR containing 50 mg/kg (P < 0.05) and influenced urea concentrations (P < 0.05). Plasma insulin (I) and IGF-I concentrations were lower in Fe-deficient calves (P < 0.05). Plasma GH in the first hours after rbGH injections increased (P < 0.05) to higher levels in calves fed 10 than in those fed 50 mg Fe/kg MR, but incremental changes were comparable. In conclusion, low Fe intake caused haematologic, metabolic and endocrine changes. Plasma IGF-I, I and T3 concentrations after rbGH administration and effects of rbGH on IGF-I in Fe-deficient calves were reduced, even though plasma GH levels were increased. PMID:7863735

Ceppi, A; Blum, J W

1994-08-01

233

Haematological, biochemical and inflammatory parameters in inactive Behçet's disease. Its association with red blood cell distribution width.  

PubMed

Red blood cell distribution width (RDW) has been shown to be associated with disease activity in several inflammatory disorders. However only one study to show this has been conducted in patients with Behçet's disease (BD). The aim of the present study was to analyse the association of RDW with BD and its main complications; i.e.; thrombosis and posterior uveitis. A second aim was to analyse the possible correlation between RDW and both haematological and inflammatory parameters. Eighty-nine patients with BD (48 males/41 females) and 94 controls (49 males/45 females) were included in the study. Patients were in an inactive phase of the disease, showing only minimum activity. RDW was statistically higher in patients than in controls (14.02 ± 1.32 vs. 13.15 ± 0.75; p < 0.001) as were CRP, fibrinogen, leucocytes and neutrophils (p < 0.001). No differences in haematimetric indices (MCV, MCH, MCHC) were observed (p > 0.05). RDW correlated negatively with haemoglobin, MCH and MCHC (p < 0.05), and directly with homocysteine (p < 0.01). No correlation was found between RDW and the several inflammatory parameters analysed (p > 0.05). The multivariate regression analysis revealed that haemoglobin and homocysteine were independent predictors of RDW (beta coefficient: -0.310; p = 0.003, beta coefficient: 0.379; p < 0.001, respectively). RDW >14 was associated with neither thrombosis nor uveitis (p = 0.935; p = 0.553, respectively). Our results indicate that BD patients show increased RDW when compared with controls. This increase seems to be related with haematimetric indices and with homocysteine levels. Lack of correlation with inflammatory markers may be due to the fact that patients were in an inactive phase of the disease. PMID:23719420

Vayá, Amparo; Rivera, Leonor; Todolí, José; Hernandez, Jose Luis; Laiz, Begoña; Ricart, José M

2014-01-01

234

Exogenous Sphingosine-1-Phosphate Boosts Acclimatization in Rats Exposed to Acute Hypobaric Hypoxia: Assessment of Haematological and Metabolic Effects  

PubMed Central

Background The physiological challenges posed by hypobaric hypoxia warrant exploration of pharmacological entities to improve acclimatization to hypoxia. The present study investigates the preclinical efficacy of sphingosine-1-phosphate (S1P) to improve acclimatization to simulated hypobaric hypoxia. Experimental Approach Efficacy of intravenously administered S1P in improving haematological and metabolic acclimatization was evaluated in rats exposed to simulated acute hypobaric hypoxia (7620m for 6 hours) following S1P pre-treatment for three days. Major Findings Altitude exposure of the control rats caused systemic hypoxia, hypocapnia (plausible sign of hyperventilation) and respiratory alkalosis due to suboptimal renal compensation indicated by an overt alkaline pH of the mixed venous blood. This was associated with pronounced energy deficit in the hepatic tissue along with systemic oxidative stress and inflammation. S1P pre-treatment improved blood oxygen-carrying-capacity by increasing haemoglobin, haematocrit, and RBC count, probably as an outcome of hypoxia inducible factor-1? mediated erythropoiesis and renal S1P receptor 1 mediated haemoconcentation. The improved partial pressure of oxygen in the blood could further restore aerobic respiration and increase ATP content in the hepatic tissue of S1P treated animals. S1P could also protect the animals from hypoxia mediated oxidative stress and inflammation. Conclusion The study findings highlight S1P’s merits as a preconditioning agent for improving acclimatization to acute hypobaric hypoxia exposure. The results may have long term clinical application for improving physiological acclimatization of subjects venturing into high altitude for occupational or recreational purposes.

Chawla, Sonam; Rahar, Babita; Singh, Mrinalini; Bansal, Anju; Saraswat, Deepika; Saxena, Shweta

2014-01-01

235

Variation in haematological parameters in children less than five years of age with asymptomatic Plasmodium infection: implication for malaria field studies  

PubMed Central

During the season of high malaria transmission, most children are infected by Plasmodium, which targets red blood cells (RBCs), affecting haematological parameters. To describe these variations, we examined the haematological profiles of two groups of children living in a malaria-endemic area. A cross-sectional survey was conducted at the peak of the malaria transmission season in a rural area of Burkina Faso. After informed consent and clinical examination, blood samples were obtained from the participants for malaria diagnosis and a full blood count. Of the 414 children included in the analysis, 192 were not infected with Plasmodium, whereas 222 were asymptomatic carriers of Plasmodium infection. The mean age of the infected children was 41.8 months (range of 26.4-57.2) compared to 38.8 months (range of 22.4-55.2) for the control group (p = 0.06). The asymptomatic infected children tended to have a significantly lower mean haemoglobin level (10.8 g/dL vs. 10.4 g/dL; p < 0.001), mean lymphocyte count (4592/µL vs. 5141/µL; p = 0.004), mean platelet count (266 x 103/µL vs. 385 x 103/µL; p < 0.001) and mean RBC count (4.388 x 106/µL vs. 4.158 x 106/µL; p < 0.001) and a higher mean monocyte count (1403/µL vs. 1192/µL; p < 0.001) compared to the control group. Special attention should be applied when interpreting haematological parameters and evaluating immune responses in asymptomatic infected children living in malaria-endemic areas and enrolled in vaccine trials.

Gansane, Adama; Ouedraogo, Issa Nebie; Henry, Noelie Bere; Soulama, Issiaka; Ouedraogo, Esperance; Yaro, Jean-Baptiste; Diarra, Amidou; Benjamin, Sombie; Konate, Amadou Tidiani; Tiono, Alfred; Sirima, Sodiomon Bienvenu

2013-01-01

236

Biochemical and haematological assessment of toxic effects of the leaf ethanol extract of Petroselinum crispum (Mill) Nyman ex A.W. Hill (Parsley) in rats  

PubMed Central

Background Petroselinum crispum, a bright green biennial shrub is widely used traditionally as a food additive and herbal remedies for many ailments. This study therefore aimed to assess the toxic effects of its leaf extract using some biochemical, haematological parameters. Methods The toxic effects were assessed by quantifying liver enzymes such as serum aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total serum protein and liver weight. Effects on haematological parameters were assessed by analysis of parked cell volume (PCV), red blood cell count (RBC), white blood cells (WBC) and haemoglobin (Hb) concentrations. Histopathological studies were done on the liver and kidneys. Results The extract caused significant increase in serum activity of alanine amino transferase and blood urea nitrogen (BUN) levels at the dose of 1000 mg/kg. Other biochemical and haematological parameters were not affected at lower doses. Conversely, the liver weight was not affected after eight weeks of treatment at the dose levels studied. The organs obtained for pathological study, were structurally unchanged under histopathological evaluation at lower doses but inflammatory and necrotic features were observed at doses???1000 mg/kg. Conclusion The results indicate that the leaf ethanol extract of Petroselinum crispum was hepatotoxic and nephrotoxic at continued oral doses equal to or more than 1000 mg/kg, but no obvious toxicity when used at lower doses. Therefore, there should be caution in its administration to avoid overdosing and known interaction with some medications. In addition, the plant should be kept away from pets and domestic animals and should not be cultivated on soil irrigated with waste water due to their ability to bio-accumulate toxic metals.

2013-01-01

237

Haematological and biochemical profile of growing Yankasa rams fed sorghum stover supplemented with graded levels of dried poultry droppings based diets.  

PubMed

This study was designed to determine the haematological and biochemical profiles of growing Yankasa rams fed sorghum stover supplemented with Sun-Dried Poultry Droppings diets (SDPD). Poultry dropping is a good source of protein supplement. Its high nitrogen content suggests feeding it to ruminant would be an excellent avenue to convert nutrients in the waste into animal products. But a major challenge for it utilization is the danger of pathogenic organisms. Sun-drying of the droppings can render the waste free of pathogens. Thirty growing Yankasa rams aged 9-12 months, weighing 11.5-15.5 kg were randomly divided into five groups (3 in each) and assigned to five experimental diets T1-T5 which contained 0, 20, 40, 60 and 80% SDPD. Blood samples were analyzed for haematological and biochemical parameters. Results showed that White Blood Cell (WBC), Haemoglobin (Hb) and Packed Cell Volume (PCV) were significantly influenced by Dried Poultry Droppings based diets (DPD). Their values were WBC, 10.6, 12.9, 9.5, 7.0 and 10.7 L(-1), Hb, 8.6, 9.3, 8.6, 8.4 and 9.7 g dL(-1) and PCV, 22.9, 29.4, 27.1, 23.6 and 21.5%, respectively. Additionally, urea, sodium and total protein were significantly influenced by treatment diet. Their values were urea, 6.1, 6.3, 6.8, 6.9 and 8.1 mg dL(-1), sodium, 102.9, 128.8, 129.2, 130.7 and 130.7 mmol L(-1). total protein, 6.3, 6.5, 7.1, 7.2 and 7.1 g dL(-1). Most haematological and biochemical values obtained were within the normal range for sheep. SDPS diet can satisfactorily supplement sorghum stover without any deleterious effect on the blood chemistry and haematological profile of growing Yankasa rams. PMID:24517007

Bello, Abdul Waheed Adeyemi; Tsado, Daniel Nma

2013-12-15

238

The relationship between haematological indices, serum gamma-glutamyl transferase and glutamate dehydrogenase, visual hepatic damage and worm burden in cattle infected with Fasciola gigantica.  

PubMed

The association between visual hepatic damage, burden of Fasciola gigantica, serum levels of gamma glutamyl transferase (GGT) and glutamate dehydrogenase (GLDH) is described from an abattoir study of 70 cattle in the Philippines. In another abattoir study of 60 cattle, the relationship between burden of F. gigantica and haematological indices was investigated. The degree of visual hepatic damage and burden of F. gigantica were significantly positively related to levels of GGT and GLDH. Red blood cell counts and packed cell volume were significantly inversely related to worm burden, but animals compensated for reduced numbers of red blood cells by increasing red cell haemoglobin content. PMID:16923272

Molina, E C; Lozano, S P; Barraca, A P

2006-09-01

239

Effect of single doses of methoxypolyethylene glycol-epoetin beta (CERA, Mircera™) and epoetin delta (Dynepo™) on isoelectric erythropoietin profiles and haematological parameters.  

PubMed

Erythropoietin (EPO) has been misused in sports for many years due to its performance-enhancing effect. In the last decade, detection of abuse has been possible with isoelectric focusing (IEF) based on the different isoform profiles of endogenous and recombinant EPO. The release of new EPOs on the market, such as the recombinant erythropoietin epoetin delta (Dynepo™) and the chemically modified EPO, CERA (Mircera™) potentially represents analytical challenges to the fight against doping. This study set out to investigate the possibility of and the time window for detecting the administration of a single dose of Dynepo™ and CERA. Our results are in agreement with earlier findings that detection of Dynepo™ is best achieved by combining IEF with SDS-PAGE. Haematological parameters were monitored for possible effects due to the long half-life (130 hours) of CERA in blood. Interestingly, although several haematological parameters were significantly changed after the injection of CERA, the endogenous EPO signal was still present in all collected samples. Due to the long half-life and the large size of the CERA molecule (about 60 kDa), it was uncertain whether CERA would be excreted into urine in detectable amounts unless urine collection was preceded by strenuous physical exercise. We find that CERA can be detected in urine without prior exercise in several, but not all, subjects. CERA is nevertheless best detected in serum with regard to both probability and length of detection, in addition to stability in matrix over time. PMID:21387570

Dehnes, Yvette; Hemmersbach, Peter

2011-05-01

240

Effect of starving and feeding on some haematological and physiological responses of the Nile catfish, Clarias gariepinus exposed to copper at extreme seasons.  

PubMed

The lethal concentration for 50% of fish for 96h (96h LC(50)) of copper (Cu(2+)) was estimated for the Nile catfish (Clarias gariepinus) in extreme seasons, winter and summer, 4.31 and 4.79 mg/l, respectively. The Nile catfish was exposed to 96h LC(50) of copper for 7 days in extreme winter and summer. The body indices, haematological parameters as well as some plasma and liver enzyme activities and metabolite level were significantly differed in fish exposed to copper over than those of the control fish. Most of the tested parameters were not significantly different between the control fish of winter and summer (winter, water temperature 18 ± 2°C and summer, 27 ± 2°C). The effect of two ration sizes on copper toxicity in two different seasons on C. gariepinus was justified. It was found that the haematological parameters and the tested plasma activities of enzymes were significantly valid due to season differences. The blood parameters as well as plasma activities of enzymes were significantly differed in fishes fed elevated ration (3%) and exposed to copper challenge. On the other hand, the exploit of low feeding ration (0.5%) along with copper exposure during the examined seasons induced non-significant differences of the tested parameters, from those of the corresponding control. Therefore, the low feeding ration provides some tolerance against the possible water-borne copper exposure. PMID:21562772

Abdel-Hameid, Nassr-Allah H

2011-12-01

241

Gastrointestinal nematode burden in working equids from humid tropical areas of central Veracruz, Mexico, and its relationship with body condition and haematological values.  

PubMed

The east coast of Veracruz, Mexico, has an important equine population used for working in rural production systems. The objectives of this study were (1) to calculate the prevalence of tropical working equids (donkeys, mules and horses) infected with gastrointestinal nematodes (GINs) and the GINs involved, and (2) to measure the body condition score (BCS) and haematological values for each working equid and its relationship with faecal worm egg count (EPG). One hundred and forty working equids were randomly selected from five different villages along the central coast of the state of Veracruz and faecal and blood samples were obtained from each animal. Gastrointestinal parasite burdens were determined using the McMaster technique. Packed cell volume, total plasma proteins, red blood cell count and white blood cell count were measured from each blood sample. Prevalence of infected equids was higher than 90 %. Mules had the highest median faecal worm egg counts (875 EPG), followed by horses and donkeys with 400 EPG. There was no correlation between EPG and BCS or haematological values (p > 0.05). Results suggest that despite the high prevalence and parasite burdens, equids involved in this trial are not being seriously affected. This study provides information which might help in designing future strategies to control nematode infections in working equids in the Mexican tropics; more emphasis should be placed on other inputs (nutrition perhaps), with individual anthelminthic treatment to those animals with the highest EPG or when signs present themselves. PMID:22992947

Valdéz-Cruz, Maura Pilar; Hernández-Gil, Mariano; Galindo-Rodríguez, Leticia; Alonso-Díaz, Miguel Angel

2013-02-01

242

Physiological responses to starvation in the European eel (Anguilla anguilla): effects on haematological, biochemical, non-specific immune parameters and skin structures.  

PubMed

The physiological effects of short-term starvation on some haematological, biochemical and non-specific immune response parameters together with the histological structure of the skin, were investigated in the European eel, Anguilla anguilla. Blood haemoglobin and haematocrit, serum glucose and cortisol, hemolysins, haemagglutinins, and lysozyme in the plasma, kidney and epidermal extract, were measured in fish after 31, 42 and 58 days of starvation, and compared to those of fed fish. Starvation did not affect haemoglobin and haematocrit values, while an increase in glucose and cortisol levels was found in starved eels by day 42. Haemolytic and haemagglutinating activities decreased in starved eels. On the other hand, starvation caused an increase in the lysozyme content in the epidermal extracts, while no significant variations were observed in kidney and plasma. On the whole, no major changes in metabolic, haematological and non-specific immune parameters were observed when short-term (less than 2 months) starvation was applied to the European eel, suggesting an adaptive response to starvation, rather than a typical alarm-stress response, allowing this species to withstand food deprivation. PMID:19093221

Caruso, G; Maricchiolo, G; Micale, V; Genovese, L; Caruso, R; Denaro, M G

2010-03-01

243

White Blood Cell Count in Women: Relation to Inflammatory Biomarkers, Haematological Profiles, Visceral Adiposity, and Other Cardiovascular Risk Factors  

PubMed Central

The role of white blood cell (WBC) count in pathogenesis of diabetes, cardiovascular disease, and obesity-related disorders has been reported earlier. Recent studies revealed that higher WBC contributes to atherosclerotic progression and impaired fasting glucose. However, it is unknown whether variations in WBC and haematologic profiles can occur in healthy obese individuals. The aim of this study is to further evaluate the influence of obesity on WBC count, inflammatory biomarkers, and metabolic risk factors in healthy women to establish a relationship among variables analyzed. The sample of the present study consisted of 84 healthy women with mean age of 35.56±6.83 years. They were categorized into two groups based on their body mass index (BMI): obese group with BMI >30 kg/m2 and non-obese group with BMI <30 kg/m2. We evaluated the relationship between WBC and platelet count (PLT) with serum interleukin 6 (IL-6), C-reactive protein (CRP), angiotensin ? (Ang ?), body fat percentage (BF %), waist-circumference (WC), and lipid profile. WBC, PLT, CRP, and IL-6 in obese subjects were significantly higher than in non-obese subjects (p< 0.05). The mean WBC count in obese subjects was 6.4±0.3 (×109/L) compared to 4.4±0.3 (×109/L) in non-obese subjects (p=0.035). WBC correlated with BF% (r=0.31, p=0.004), CRP (r=0.25, P=0.03), WC (r=0.22, p=0.04), angiotensin ? (r=0.24, p=0.03), triglyceride (r=0.24, p=0.03), and atherogenic index of plasma (AIP) levels (r=0.3, p=0.028) but not with IL-6. Platelet count was also associated with WC and waist-to-hip ratio (p<0.05). Haemoglobin and haematocrit were in consistent relationship with LDL-cholesterol (p<0.05). In conclusion, obesity was associated with higher WBC count and inflammatory parameters. There was also a positive relationship between WBC count and several inflammatory and metabolic risk factors in healthy women.

Farhangi, Mahdieh Abbasalizad; Keshavarz, Seyyed-Ali; Eshraghian, Mohammadreza; Ostadrahimi, Alireza

2013-01-01

244

White blood cell count in women: relation to inflammatory biomarkers, haematological profiles, visceral adiposity, and other cardiovascular risk factors.  

PubMed

The role of white blood cell (WBC) count in pathogenesis of diabetes, cardiovascular disease, and obesity-related disorders has been reported earlier. Recent studies revealed that higher WBC contributes to atherosclerotic progression and impaired fasting glucose. However, it is unknown whether variations in WBC and haematologic profiles can occur in healthy obese individuals. The aim of this study is to further evaluate the influence of obesity on WBC count, inflammatory biomarkers, and metabolic risk factors in healthy women to establish a relationship among variables analyzed. The sample of the present study consisted of 84 healthy women with mean age of 35.56 +/- 6.83 years. They were categorized into two groups based on their body mass index (BMI): obese group with BMI > 30 kg/m2 and non-obese group with BMI < 30 kg/m2. We evaluated the relationship between WBC and platelet count (PLT) with serum interleukin 6 (IL-6), C-reactive protein (CRP), angiotensin pi (Ang pi), body fat percentage (BF %), waist-circumference (WC), and lipid profile. WBC, PLT, CRP, and IL-6 in obese subjects were significantly higher than in non-obese subjects (p < 0.05). The mean WBC count in obese subjects was 6.4 +/- 0.3 (x10(9)/L) compared to 4.4 +/- 0.3 (x10(9)/L) in non-obese subjects (p = 0.035). WBC correlated with BF% (r = 0.31, p = 0.004), CRP (r = 0.25, P = 0.03), WC (r = 0.22, p = 0.04), angiotensin 11 (r = 0.24, p = 0.03), triglyceride (r = 0.24, p = 0.03), and atherogenic index of plasma (AIP) levels (r = 0.3, p = 0.028) but not with IL-6. Platelet count was also associated with WC and waist-to-hip ratio (p < 0.05). Haemoglobin and haematocrit were in consistent relationship with LDL-cholesterol (p < 0.05). In conclusion, obesity was associated with higher WBC count and inflammatory parameters. There was also a positive relationship between WBC count and several inflammatory and metabolic risk factors in healthy women. PMID:23617205

Farhangi, Mahdieh Abbasalizad; Keshavarz, Seyyed-Ali; Eshraghian, Mohammadreza; Ostadrahimi, Alireza; Saboor-Yaraghi, Ali-Akbar

2013-03-01

245

Hand-held dynamometry in patients with haematological malignancies: Measurement error in the clinical assessment of knee extension strength  

PubMed Central

Background Hand-held dynamometry is a portable and inexpensive method to quantify muscle strength. To determine if muscle strength has changed, an examiner must know what part of the difference between a patient's pre-treatment and post-treatment measurements is attributable to real change, and what part is due to measurement error. This study aimed to determine the relative and absolute reliability of intra and inter-observer strength measurements with a hand-held dynamometer (HHD). Methods Two observers performed maximum voluntary peak torque measurements (MVPT) for isometric knee extension in 24 patients with haematological malignancies. For each patient, the measurements were carried out on the same day. The main outcome measures were the intraclass correlation coefficient (ICC ± 95%CI), the standard error of measurement (SEM), the smallest detectable difference (SDD), the relative values as % of the grand mean of the SEM and SDD, and the limits of agreement for the intra- and inter-observer '3 repetition average' and the 'highest value of 3 MVPT' knee extension strength measures. Results The intra-observer ICCs were 0.94 for the average of 3 MVPT (95%CI: 0.86–0.97) and 0.86 for the highest value of 3 MVPT (95%CI: 0.71–0.94). The ICCs for the inter-observer measurements were 0.89 for the average of 3 MVPT (95%CI: 0.75–0.95) and 0.77 for the highest value of 3 MVPT (95%CI: 0.54–0.90). The SEMs for the intra-observer measurements were 6.22 Nm (3.98% of the grand mean (GM) and 9.83 Nm (5.88% of GM). For the inter-observer measurements, the SEMs were 9.65 Nm (6.65% of GM) and 11.41 Nm (6.73% of GM). The SDDs for the generated parameters varied from 17.23 Nm (11.04% of GM) to 27.26 Nm (17.09% of GM) for intra-observer measurements, and 26.76 Nm (16.77% of GM) to 31.62 Nm (18.66% of GM) for inter-observer measurements, with similar results for the limits of agreement. Conclusion The results indicate that there is acceptable relative reliability for evaluating knee strength with a HHD, while the measurement error observed was modest. The HHD may be useful in detecting changes in knee extension strength at the individual patient level.

Knols, Ruud H; Aufdemkampe, Geert; de Bruin, Eling D; Uebelhart, Daniel; Aaronson, Neil K

2009-01-01

246

Haematological changes at altitude.  

PubMed

Hypobaric hypoxia is associated with an increase in erythropoesis and an increased thrombotic risk. This is true of long haul air travel, mountaineering expeditions and longer stays at altitude. Studies looking at clotting on mountaineering expeditions are further complicated by the effects of exercise, plasma volume changes and the catecholamine response to hypoxia. This review examines the evidence for changes in clotting factors and functional clotting at altitude and considers the implications of altitude travel for those with pre-existing medical conditions. PMID:21465909

Wheatley, K; Creed, M; Mellor, A

2011-03-01

247

Splenectomy for haematological disorders.  

PubMed

(Full text is available at http://www.manu.edu.mk/prilozi). Splenectomy is therapeutic for a large host of conditions. It is a consequence of expanding the list of disorders and liberalizing the indications for splenectomy in many diseases. Red blood cells disorders: autoimmune hemolytic anemia, hereditary spherocytosis, hemoglobinopathies and thalassemia are prone to splenectomy after failure of medical therapy. A variety of thrombocytopenic disorders are improved by splenectomy, and the most common indication for splenectomy is ITP (idiopathic thrombocytopenic purpura). Splenectomy is successful in reversing hypersplenism in a spectrum of disease called myeloproliferative disorders. Relief of symptoms from splenomegaly is also achieved, but it does not affect the inexorable course of the disorder. The role of splenectomy in white blood cells disorders (leukemias and lymphomas) is only palliative and facilitates chemotherapy. Splenectomy in patients with hemathologic disorders imparts a risk of fulminant and life threatening infection "overwhelming postsplenectomy sepsis" that can be obviated by appropriate treatment. Although splenectomy for hemathologic disorders is only therapeutic and not curative, the relief of symptoms and for some disorders facilitation of chemotherapy leads to better quality of life and longer survival. Key words: Splenectomy, laparoscopic splenectomy, hemathologic dsorders, hereditary spherocytosis, idio-pathic thrombocytopenic purpura, ITP, myeloproliferative disorders, lymphoma, overwhelming posts-plenectomy sepsis. PMID:24798604

Jankulovski, N; Antovic, S; Kuzmanovska, B; Mitevski, A

2014-01-01

248

Effects of preoperative administration of meloxicam on whole blood platelet aggregation, buccal mucosal bleeding time, and haematological indices in dogs undergoing elective ovariohysterectomy.  

PubMed

The purpose of the study was to evaluate the effect of preoperative administration of meloxicam, a non-steroidal anti-inflammatory drug used for pain control, on primary haemostasis in dogs. Twenty healthy female dogs undergoing elective ovariohysterectomy were enrolled in the study. Sixty minutes before pre-anaesthesia, a single dose of meloxicam (0.2 mg/kg) was randomly administered intravenously (IV) to 10 dogs (treatment group) while control dogs received an equivalent volume of saline solution IV. Platelet aggregation, buccal mucosa bleeding time, platelet count and haematological indices were measured at 0, 1, 6 and 24 h after administration of meloxicam. Since significant differences between groups were not observed for any of the measured parameters, preoperative administration of meloxicam may be used for pain control before elective ovariohysterectomy in healthy dogs, without compromising primary haemostasis. PMID:15993799

Fresno, Laura; Moll, Javier; Peñalba, Begoña; Espada, Yvonne; Andaluz, Anna; Prandi, David; Ruiz de Gopegui, Rafael; García, Félix

2005-07-01

249

Object-oriented business process analysis of the cooperative soft tissue sarcoma trial of the german society for paediatric oncology and haematology (GPOH).  

PubMed

The German Society for Paediatric Oncology and Haematology (GPOH) runs nation-wide multicentre clinical trials to improve the treatment of children suffering from malignant diseases. We want to provide methods and tools to support the centres of these trials in developing trial specific modules for the computer-based DOcumentation System for Paediatric Oncology (DOSPO). For this we carried out an object-oriented business process analysis for the Cooperative Soft Tissue Sarcoma Trial at the Olgahospital Stuttgart for Child and Adolescent Medicine. The result is a comprehensive business process model consisting of UML-diagrams and use case specifications. We recommend the object-oriented business process analysis as a method for the definition of requirements in information processing projects in the field of clinical trials in general. For this our model can serve as basis because it slightly can be adjusted to each type of clinical trial. PMID:11604706

Weber, R; Knaup, P; Knietitg, R; Haux, R; Merzweiler, A; Mludek, V; Schilling, F H; Wiedemann, T

2001-01-01

250

Changes in haematology measurements with the Sysmex XT-2000iV during storage of feline blood sampled in EDTA or EDTA plus CTAD.  

PubMed

In veterinary medicine a complete blood cell count (CBC) cannot always be performed within 24 h as usually recommended, particularly for specimens shipped to a reference laboratory. This raises the question of the stability of the variables, especially in ethylenediamine tetra-acetic acid (EDTA) feline blood specimens, known to be prone to in vitro platelet aggregation. Citrate, theophylline, adenosine and dipyridamole (CTAD) has been reported to limit platelet aggregation in feline blood specimens. The aim of this study was to measure the stability of the haematological variables and the platelet aggregation score in EDTA and EDTA plus CTAD (EDCT) feline blood specimens during 48 h of storage at room temperature. Forty-six feline EDTA and EDCT blood specimens were analysed with a Sysmex XT-2000iV analyser, and the platelet count and score of platelet aggregation were estimated immediately and after 24 and 48 h of storage. A significant increase in mean corpuscular volume, haematocrit, reticulocyte and eosinophil counts, and a significant decrease in mean corpuscular haemoglobin concentration and monocyte count were observed. Haemoglobin, mean corpuscular haemoglobin, and red blood cell, white blood cell, neutrophil and lymphocyte counts remained stable. Changes in reticulocyte indexes with time (low fluorescence ratio, medium fluorescence ratio, high fluorescence ratio and immature reticulocyte fraction) were not significant. Changes were generally more pronounced in EDTA than in EDCT. Platelet aggregation decreased markedly in initially highly aggregated EDTA specimens, and increased slightly in initially non- or mildly-aggregated EDTA or EDCT specimens. Platelet counts increased and decreased, or remained stable, respectively. CTAD can reduce storage-induced changes of the haematological variables in feline samples, thus improving the reliability of a CBC and limiting clinical misinterpretations. PMID:23264612

Granat, Fanny; Geffré, Anne; Bourgès-Abella, Nathalie; Braun, Jean-Pierre; Trumel, Catherine

2013-06-01

251

Anticancer Effect of dl-Glyceraldehyde and 2-Deoxyglucose in Ehrlich Ascites Carcinoma Bearing Mice and Their Effect on Liver, Kidney and Haematological Parameters.  

PubMed

Cancer cells generally exhibit increased glycolysis for ATP generation (the Warburg effect). Compounds that inhibit glycolysis have potential applications in cancer treatment. dl-glyceraldehyde (DLG) and 2-Deoxyglucose (2-DG) have been proven effective in the inhibition of glucose metabolism. Ehrlich ascites carcinoma (EAC) cells were injected intraperitoneally (i.p) in 10-12 weeks old Swiss albino mice, weighing between 20 and 30 g. The anticancer activity of DLG and 2-DG were determined by tumor volume, tumor weight, viable and nonviable tumor cell count, average survival time, percentage increase in life span and tumor inhibition ratio. The blood samples were obtained for biochemical analysis after 9 days of treatment to study the effect on liver, kidney and haematological parameters. Histopathological examination of liver and kidney was also performed. One-way ANOVA test and Dunnett's test were used for comparisons of parameters in study groups. Both DLG and 2-DG individually decreased the tumor weight, tumor volume, viable tumor cell count and significantly increased the life span of treated mice, however the combination was found to be better. The biochemical parameters of liver and kidney functions and haematological parameters were restored close to control group as compared with the EAC bearing mice. Histopathological examination of liver and kidney in EAC control group showed large areas of necrosis, congestion and mononuclear cell infiltration but such changes were not observed in liver and kidney sections observed after i.p injection of DLG and 2-DG for 9 days. Improvement was much better in the group where combination of these two drugs were used. PMID:24757305

Kapoor, R; Gundpatil, D B; Somani, B L; Saha, T K; Bandyopadhyay, S; Misra, P

2014-04-01

252

Experimental Studies on the Hypolipidemic and Haematological Properties of Aqueous Leaf Extract of Cleistopholis Patens Benth. & Diels. (Annonacae) in Hypercholesterolemic Rats  

PubMed Central

Background: Cleistopholis patens (Cp) (Annonacae) is a popular medicinal herb used in the the treatment of cardiovascular disorders in Nigerian ethnomedicine. Objectives: The present study investigated the effects of the aqueous leaf extract of Cp on lipid and haematological profile of hypercholesterolemic Albino rats. Methods: The animals were divided into 5 groups (A – E) of 5 animals per group. All the animals in groups A – D, along with their normal diet, also received 400 mg/kg of cholesterol suspended in 2 % Tween 80 (p.o) daily for 60 days. Six hours after each cholesterol feeding, the animals were treated respectively with a hypolipodemic drug, simvastatin; 10 mg/kg, C. patens extract; 400 mg/kg, 600 mg/kg and 2 % Tween 80 (negative control). The last group (positive control) received cholesterol free diet and 2 % Tween 80. All the treatments were administered orally for a period of 60 days. Thereafter, the lipid and haematological profile of serum and blood samples collected were determined respectively. Results: Treatment with simvastatin (10 mg/kg), the extract (400 and 600 mg/kg) significantly (P =0.033) lowered the total cholesterol, triglycerides, low density lipoproteins (LDL) levels and significantly (P =0.05) increased the level of high density lipoproteins (HDL) in comparison with the negative control group. There was no significant difference between the cholesterol lowering effect of the extract and that of simvastatin (P =0.991). The haemoglobin concentration, packed cell volume, red and white blood cell counts of treated animals did not produce any significant change (P =0.705). The LD50 showed that the extract has a wide margin of safety. Conclusion: The extract showed marked hypolipidemic activity.

Udem, SC; Ezeonuegbu, UC; Obidike, RI

2011-01-01

253

Left ventricular remodelling, and systolic and diastolic function in young adults with ? thalassaemia major: a Doppler echocardiographic assessment and correlation with haematological data  

PubMed Central

Objective: To evaluate left ventricular morphology and function in a large population of patients with ? thalassaemia. Design: Echo Doppler assessment of left ventricular function and correlation of cardiovascular data with haematological data. Setting: Thalassaemia unit in a tertiary referral centre. Patients: 197 young adults with ? thalassaemia, following an adequate transfusional and chelation treatment regimen, without clinical signs of cardiopulmonary involvement. The control group consisted of 213 healthy subjects. Results: Left ventricular volumes, mass index, and mass/volume ratio were increased. Diastolic and systolic shapes were different, the left ventricle maintaining an ellipsoidal shape. The ejection fraction was reduced, and was < 50% in 33 patients. Stroke volume and cardiac index were increased, and systemic vascular resistance was decreased. Fractional shortening and mean velocity of circumferential shortening were decreased. Meridional end systolic and peak systolic stress were increased, as was circumferential end systolic stress. The contractile state was reduced while the functional preload index did not differ. Left ventricular diastolic function, evaluated from the mitral inflow, showed a slightly prolonged isovolumic relaxation time, increased flow velocity integrals, and an increased E/A ratio. Among the haematological data, only serum ferritin showed a weak negative correlation with left ventricular ejection fraction. The patients with the highest serum ferritin (> 2500 ng/ml) had the lowest ejection fraction. Conclusions: Patients with ? thalassaemia on an adequate transfusion and chelation treatment regimen show abnormal left ventricular remodelling with increased volumes, mass, and mass/volume ratio. Systolic chamber function and contractile state are reduced, with a slightly increased afterload. These findings seem mainly to be related to the increased cardiac output caused by chronic anaemia. Left ventricular performance is better preserved when chelation treatment is adjusted to maintain the serum ferritin concentration at < 1000 ng/ml.

Bosi, G; Crepaz, R; Gamberini, M R; Fortini, M; Scarcia, S; Bonsante, E; Pitscheider, W; Vaccari, M

2003-01-01

254

Mucormycosis in patients with haematological malignancies: a retrospective clinical study of 37 cases. GIMEMA Infection Program (Gruppo Italiano Malattie Ematologiche Maligne dell'Adulto).  

PubMed

A retrospective study of 37 patients with haematological malignancy (21 acute myeloid leukaemia, 11 acute lymphoid leukaemia, two lymphoma, two hairy cell leukaemia, one Hodgkin's disease) and histologically documented mucormycosis was conducted to evaluate the clinical characteristics and ascertain the factors which influenced the outcome from mycotic infection. Patients were admitted to 18 haematology divisions in tertiary care or university hospitals in Italy between 1987 and 1995. Fever, thoracic pain, dyspnoea and cough were the most frequent presenting symptoms. At the onset, 89% patients were neutropenic (neutrophil counts < 0.5 x 10(9)/l) with a median duration of previous neutropenia of 14 d (range 6-60). The most frequent sites of infection were lungs (81%), CNS (27%), sinus (16%), liver (16%) and orbital space (10%). Only three patients were asymptomatic. A correct in vivo diagnosis was made in only 13 (35%) patients. When performed, thoracic and cranial CT scan were the most useful diagnostic investigations. Despite the fact that 26 febrile patients were treated with empirical antifungal treatment, 28 of the 37 patients (76%) died from fungal infection at a median time of 17 d from the onset of clinical symptoms. Nine patients were cured by antifungal therapy plus, in five cases, radical surgery procedures. An analysis of factors influencing outcome demonstrated that the resolution of chemotherapy-induced neutropenia and prolonged treatment with amphotericin B and, if feasible, radical surgical debridement treatment, were significantly correlated with recovery from infection. Mucormycosis, a rare filamentous fungal infection that occurs most frequently in neutropenic acute leukaemia patients, is characterized by a high mortality rate. Extensive and aggressive diagnostic and therapeutic procedures are essential to improve the prognosis in these patients. PMID:9375750

Pagano, L; Ricci, P; Tonso, A; Nosari, A; Cudillo, L; Montillo, M; Cenacchi, A; Pacilli, L; Fabbiano, F; Del Favero, A

1997-11-01

255

Modification of endotoxin-induced haemodynamic and haematological changes in the rabbit by methylprednisolone, F(ab')2 fragments and rosmarinic acid.  

PubMed Central

The effects of methylprednisolone, F(ab')2 fragments of human gamma globulins and rosmarinic acid, an inhibitor of complement activation, were tested on endotoxin-induced haemodynamic and haematological changes in the rabbit. Their effects were compared with complement depletion by cobra venom factor (CVF) pretreatment. The results provide further evidence for the role of complement activation and the concomitant triggering of the arachidonic acid cascade in the early phase of shock. The formation of vasoactive prostanoids (prostacyclin and thromboxane A2), the arterial hypotension and the thrombocytopenia were largely dependent on the presence of the intact complement system. F(ab')2 fragments (150 mg kg-1, i.v.) diminished the second fall in blood pressure to some extent but failed to alter any of the other endotoxin-induced changes. Methylprednisolone (40 mg kg-1, i.v.) given 10 min before endotoxin significantly reduced the activation of complement, the second rise of prostacyclin and the secondary hypotension, but was without effect on the early thromboxane peak of the haematological features of endotoxin shock. Rosmarinic acid (20 mg kg-1, i.v.) may be of potential interest for treatment of septic shock, since the drug suppressed the endotoxin-induced activation of complement, the formation of prostacyclin, both hypotensive phases, the thrombocytopenia and the concomitant release of thromboxane A2. The role of leukocytes and their arachidonic acid metabolites in plasma exudation deserves further investigation, because leukopenia and pulmonary oedema were not complement-dependent and were not affected by any of the treatments. Our results indicate that drugs, interfering with complement activation and/or prostaglandin biosynthesis, may be beneficial in endotoxin shock, provided that they are administered at an early stage.

Bult, H.; Herman, A. G.; Rampart, M.

1985-01-01

256

Association of interferon regulatory factor 4 gene polymorphisms rs12203592 and rs872071 with skin cancer and haematological malignancies susceptibility: a meta-analysis of 19 case-control studies  

PubMed Central

Background Research has indicated that the rs12203592 and rs872071 interferon regulatory factor 4 (IRF4) gene polymorphisms correlate with the risk of cancer, especially skin cancer and haematological malignancies, but the results remain controversial. To understand better the effects of these two polymorphisms on skin cancer and haematological malignancies susceptibility, a cumulative meta-analysis was performed. Methods We conducted a search using the PubMed and Web of Science databases for relevant case-control studies published before April 2014. Summary odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were estimated using fixed- or random-effects models where appropriate. Heterogeneity test, publication bias test, and sensitivity analysis were also performed. Results In total, 11 articles comprised of 19 case–control studies were identified; five focused on the rs12203592 polymorphism with 7,992 cases and 8,849 controls, and six were on the rs872071 polymorphism with 3108 cases and 8300 controls. As for rs12203592, a significant correlation with overall skin cancer and haematological malignancies risk was found with the homozygote comparison model (OR?=?1.566, 95% CI 1.087-2.256) and recessive model (OR?=?1.526, 95% CI 1.107-2.104). For rs872071, a significantly elevated haematological malignancies risk was observed in all genetic models (homozygote comparison: OR?=?1.805, 95% CI 1.402-2.323; heterozygote comparison: OR?=?1.427, 95% CI 1.203-1.692; dominant: OR?=?1.556, 95% CI 1.281-1.891; recessive: OR?=?1.432, 95% CI 1.293-1.587; additive: OR?=?1.349, 95% CI 1.201-1.515). Similarly, increased skin cancer and haematological malignancies risk was also identified after stratification of the SNP data by cancer type, ethnicity and source of controls for both polymorphisms. Conclusions Our meta-analysis indicated that the rs12203592 and rs872071 IRF4 gene polymorphisms are associated with individual susceptibility to skin cancer and haematological malignancies. Moreover, the effect of the rs12203592 polymorphism on skin cancer risk was particularly prominent among Caucasians. Further functional research should be performed to validate the association.

2014-01-01

257

Immuno-haematologic and virologic responses and predictors of virologic failure in HIV-1 infected adults on first-line antiretroviral therapy in Cameroon  

PubMed Central

Background Contemporary data on the immunologic, haematologic and virologic responses and predictors of virologic failure after initiation of free antiretroviral treatment in Cameroon are needed to evaluate the current treatment-monitoring algorithm and to complement efforts to scale-up and improve on the management of HIV infections. Methods This was a cross-sectional study conducted between October 2010 and June 2012. A total of 951 participants aged 18–74 years were recruited from selected approved HIV treatment centres of the Northwest and Southwest regions. This comprised 247 males and 704 females. Demographic, self-reported risk behaviours and socioeconomic data were obtained using a structured questionnaire. Full blood and CD4?+?T-cell counts were done using standard automated techniques. Determination of viral load (VL) was done using Abbott RealTime HIV-1?m2000™ system. Data was analysed using SPSS version 17. The statistical significance level was P??500 cells/?L respectively. Anaemia was present in 26.2% of the participants with 62.3%, 25.7% and 12% described as mild, moderate and severe anaemia respectively. Virologic failure occurred in 23.2% of the participants with 12.3% having VL?>?10,000 RNA copies/mL. Meanwhile 76.8% of patients attained adequate viral suppression with 40.8% having undetectable viral load. The age group 18–29 years (p?=?0.024), co-infection with tuberculosis (p?=?0.014), anaemia (p?=?0.028) and distance from the treatment centre (p?=?0.011) independently predicted virologic failure. Conclusion The majority of the participants achieved adequate viral suppression after???6 months of ART. Despite these favourable immuno-haematologic and virologic outcomes, the National AIDS Control Program should step-up efforts to improve on antiretroviral drug distribution, as well as proper assessment and management of anaemia, foster early diagnosis and treatment of tuberculosis and enhance treatment adherence counselling especially in younger patients.

2014-01-01

258

The role of surgery in the treatment of invasive fungal infection in paediatric haematology patients: a retrospective single-centre survey.  

PubMed

Surgery may improve the control of fungal disease and patient survival. The aim of this study was to report a single-centre experience in using surgery for the treatment of paediatric invasive fungal infection (IFI). From 2001 to 2009, 18 paediatric onco-haematology patients underwent 24 surgical procedures as treatment of IFI. At surgery, severe thrombocytopenia and neutropenia were present in four and one episodes respectively. Complications were one pleural effusion, one pleural effusion and surgical wound infection, one pneumothorax with wound dehiscence and one wound dehiscence. None of them required repeat surgery. The median duration of hospitalisation for four complicated procedures was 11 days, range 3-16, and 7 days, range 2-13, for the 20 uncomplicated procedures. No surgery-related deaths occurred. Fourteen patients resumed chemotherapy after a median of 26 days, range 9-77, whereas nine patients underwent hematopoietic stem cell transplantation after a median of 42 days, range 27-110. At 3 months from IFI, 17 patients were alive (94%) and one patient (6%) died from mycosis; the 3-month overall survival (OS) being 94.4%, CI 66.6-99.2. After a median follow-up of 7.1 years (CI 2.8-7.5), the OS was 54.5%, CI 29.2-74.2. Surgery is a feasible and valuable option in paediatric patients because it is associated with a low incidence of complications and an acceptable delay in resuming the chemotherapeutic plan. PMID:24438353

Cesaro, Simone; Pegoraro, Anna; Tridello, Gloria; Pillon, Marta; Cannata, Elisa; Faggin, Stefano; Cecchetto, Giovanni

2014-07-01

259

Novel methicillin-resistant coagulase-negative Staphylococcus clone isolated from patients with haematological diseases at the Blood Bank Centre of Amazon, Brazil.  

PubMed

Methicillin-resistant Staphylococcus remains a severe public health problem worldwide. This research was intended to identify the presence of methicillin-resistant coagulase-negative staphylococci clones and their staphylococcal cassette chromosome mec (SCCmec)-type isolate from patients with haematologic diseases presenting bacterial infections who were treated at the Blood Bank of the state of Amazonas in Brazil. Phenotypic and genotypic tests, such as SCCmec types and multilocus sequence typing (MLST), were developed to detect and characterise methicillin-resistant isolates. A total of 26 Gram-positive bacteria were isolated, such as: Staphylococcus epidermidis (8/27), Staphylococcus intermedius (4/27) and Staphylococcus aureus (4/27). Ten methicillin-resistant staphylococcal isolates were identified. MLST revealed three different sequence types: S. aureus ST243, S. epidermidis ST2 and a new clone of S. epidermidis, ST365. These findings reinforce the potential of dissemination presented by multi-resistant Staphylococcus and they suggest the introduction of monitoring actions to reduce the spread of pathogenic clonal lineages of S. aureus and S. epidermidis to avoid hospital infections and mortality risks. PMID:23579805

Ferreira, Cristina Motta; Naveca, Felipe Gomes; Ferreira, William Antunes; de Oliveira, Cíntia Mara Costa; Barbosa, Maria das Graças Vale

2013-04-01

260

Volume-dependent collection of peripheral blood progenitor cells during large-volume leukapheresis for patients with solid tumours and haematological malignancies.  

PubMed

We investigated the efficacy of peripheral blood progenitor cell (PBPC) collection during large-volume leukapheresis (LVL) in patients with solid tumours and haematological malignancies (n = 18). The time- and volume-dependent harvest of leucocytes (WBC), mononuclear cells (MNC), CD34+ cells and colony-forming cells (CFU-GM) during LVL was analysed in six sequentially filled collection bags processing four times the patient's blood volumes. The amounts of leucocytes (WBC) and the purity of mononuclear cells (MNC%) did not show any significant changes during LVL. The percentage of CD34+ cells remained constant for the first three bags but consecutively decreased from initially 1.71% CD34+ cells in the beginning of LVL to finally 1.34% CD34+ cells (P = 0.02). The mean numbers of colony-forming cells (CFU-GM) decreased from 74 microL-1 to 59 microL-1 during LVL (P = 0.16). Furthermore, the comparison of volume-dependent PBPC collection for patients with high, medium and low total yields of CD34+ cells showed similar kinetics on different levels for the three groups. We concluded that - relative to the initial total amount of PBPC harvested - comparable numbers of progenitor cells can be collected during all stages of LVL with a slight decreasing trend processing four times the patient's blood volumes. PMID:10583885

Cassens, U; Ostkamp-Ostermann, P; van der Werf, N; Garritsen, H; Ostermann, H; Sibrowski, W

1999-12-01

261

A rescue therapy with a combination of caspofungin and liposomal amphotericin B or voriconazole in children with haematological malignancy and refractory invasive fungal infections.  

PubMed

Combination treatment of paediatric invasive fungal infections (IFIs) has rarely been reported. A total of 17 children with 19 IFI episodes were enrolled in the study. The median age of the patients was 5.3 (range 0.5-17) years. IFI was classified as proven in 4, probable in 12 and possible in 3 episodes. These patients received empiric antifungal treatment, which consisted of liposomal amphotericin B (LAmB) monotherapy for a median duration of 12 days (range 3-69 days). All patients were refractory to LAmB; therefore, caspofungin was added to the therapy in 11 patients. In the remaining six patients, LAmB was ceased and a combination of caspofungin and voriconazole was started. Among the patients who received caspofungin + LAmB, four did not show favourable response and the combination was switched to caspofungin + voriconazole. The median (range) and total duration of the therapy were 7 (3-14) days and 91 patient days for LAmB + caspofungin combination and 49 (7-126) days and 516 patient days for caspofungin + voriconazole combination. We found a favourable response rate of 68.4% in 16 proven or probable IFI episodes. Twelve-week survival rate of these patients was 75%. No serious side effect was observed among the patients. Our data suggest that combination antifungal therapy is safe and effective in children with haematological malignancies. PMID:19906090

Yilmaz, Deniz; Balkan, Can; Ay, Yilmaz; Akin, Mehmet; Karapinar, Bulent; Kavakli, Kaan

2011-05-01

262

A phase I first-in-human study with tefinostat - a monocyte/macrophage targeted histone deacetylase inhibitor - in patients with advanced haematological malignancies.  

PubMed

Tefinostat (CHR-2845) is a monocyte/macrophage targeted histone deacetylase inhibitor (HDACi). This first-in-human, standard 3 + 3 dose escalating trial of oral, once daily tefinostat was conducted to determine the safety, tolerability, pharmacokinetic and pharmacodynamic profile of tefinostat in relapsed/refractory haematological diseases. Eighteen patients were enrolled at doses of 20-640 mg. Plasma concentrations of tefinostat exceeded those demonstrated to give in vitro anti-proliferative activity. Flow cytometric pharmacodynamic assays demonstrated monocyte-targeted increases in protein acetylation, without corresponding changes in lymphocytes. Dose-limiting toxicities (DLTs) were not observed and dose escalation was halted at 640 mg without identification of the maximum tolerated dose. Drug-related toxicities were largely Common Toxicity Criteria for Adverse Events grade 1/2 and included nausea, anorexia, fatigue, constipation, rash and increased blood creatinine. A patient with chronic monomyelocytic leukaemia achieved a bone marrow response, with no change in peripheral monocytes. An acute myeloid leukaemia type M2 patient showed a >50% decrease in bone marrow blasts and clearance of peripheral blasts. In conclusion, tefinostat produces monocyte-targeted HDACi activity and is well tolerated, without the DLTs, e.g. fatigue, diarrhoea, thrombocytopenia, commonly seen with non-targeted HDACi. The early signs of efficacy and absence of significant toxicity warrant further evaluation of tefinostat in larger studies. (clinicaltrials.gov identifier: NCT00820508). PMID:23647373

Ossenkoppele, Gert J; Lowenberg, Bob; Zachee, Pierre; Vey, Norbert; Breems, Dimitri; Van de Loosdrecht, Arjan A; Davidson, Alan H; Wells, Graham; Needham, Lindsey; Bawden, Lindsay; Toal, Martin; Hooftman, Leon; Debnam, Phillip M

2013-07-01

263

Susceptibility to levofloxacin of clinical isolates of bacteria from intensive care and haematology/oncology patients in Switzerland: a multicentre study.  

PubMed

The objective of this study was to examine the susceptibility of clinical isolates to levofloxacin, a fluoroquinolone with extended activity against Gram-positive bacteria, and other antibiotics in 12 Swiss clinical microbiology laboratories using the NCCLS disc diffusion technique. Isolates were prospectively collected from intensive care units (ICUs (59%), oncology wards (7%) and other units with haematology/oncology patients (34%) from June 1995 to March 1996. The levofloxacin breakpoints used were as recommended by the manufacturer. A total of 310 Gram-positive and 580 Gram-negative isolates from the respiratory tract (36%), skin/wounds (12%), blood (16%), urine (17%) and other sources (19%) were tested. The percentage of isolates susceptible to levofloxacin was 100% for Enterococcus spp. (38 strains), Streptococcus agalactiae (13), Streptococcus pneumoniae (65), Acinetobacter spp. (11), Citrobacter diversus (6), Citrobacter freundii (17), Klebsiella oxytoca (39), Morganella morganii (16), Proteus mirabilis (20), Proteus vulgaris (23), Serratia spp. (19), Stenotrophomonas maltophilia (10) and Haemophilus influenzae (41). The percentage of isolates susceptible to levofloxacin for Staphylococcus aureus (95 strains, including 2% MRSA) was 94%, coagulase-negative staphylococci (85) 65%, Enterobacter spp. (75) 99%, Escherichia coli (111) 97%, Klebsiella pneumoniae (45) 98% and Pseudomonas aeruginosa (124) 87%. In conclusion, levofloxacin is a new fluoroquinolone to which the most common clinical isolates in Switzerland are susceptible. The susceptibility of Enterococcus spp. and S. pneumoniae to levofloxacin was particularly remarkable. This compound appears to be a promising therapeutic alternative for the treatment of Gram-positive infections. PMID:10404338

Siegrist, H H; Nepa, M C; Jacquet, A

1999-06-01

264

Response to short term starvation of growth, haematological, biochemical and non-specific immune parameters in European sea bass (Dicentrarchus labrax) and blackspot sea bream (Pagellus bogaraveo).  

PubMed

Growth, haematological (haematocrit), biochemical (serum cortisol and glucose), and non-specific immune (lysozyme, serum haemolytic and haemagglutinating activities, extracellular respiratory burst activity) parameters, were monitored in European sea bass Dicentrarchus labrax and blackspot sea bream Pagellus bogaraveo subjected to a 31 days starvation compared to fed fish, to assess the responses to feed deprivation of these health status indicators. While haematocrit, serum cortisol, glucose and haemolytic activity of both species did not undergo significant variation following starvation, probably due to the short period applied, some non-specific immune parameters were affected significantly. In the starved sea bass, mucus lysozyme content doubled (1.8 U/mL) compared to the initial value. Haemagglutinating activity was significantly lower in starved sea bass than in fed fish after 31 days. In blackspot sea bream, a slight, not significant, reduction in haemagglutinating activity occurred 11 days after starvation. Respiratory burst activity decreased significantly in the starved fish. In spite of the limited number of examined parameters, the opportunity to use a panel of several indicators to obtain a more complete picture of health status in fish was underlined. PMID:21664688

Caruso, Gabriella; Denaro, Maria Gabriella; Caruso, Rosalba; Mancari, Ferdinando; Genovese, Lucrezia; Maricchiolo, Giulia

2011-07-01

265

Central venous catheter-associated bloodstream infection and colonisation of insertion site and catheter tip. What are the rates and risk factors in haematology patients?  

PubMed

Skin colonisation is an important source for central venous catheter (CVC) colonisation and infection. This study intended to identify risk factors for skin colonisation prior to CVC placement (baseline colonisation) and within 10 days after CVC insertion (subsequent colonisation), for CVC-tip colonisation and for bloodstream infection (BSI). Within a randomised clinical trial, data of 219 patients with haematological malignancies and inserted CVC (with a total of 5,501 CVC-days and 4,275 days at risk) in two university hospitals were analysed. Quantitative skin cultures were obtained from the insertion site before CVC placement and at regular intervals afterwards. CVC-tip cultures were taken on CVC removal and data collection was performed. Statistical analysis included linear and logistic regression models. Age was an independent risk factor for colonisation prior to CVC placement (baseline colonisation). Independent risk factors for subsequent colonisation were baseline colonisation and male gender. High level of subsequent skin colonisation at the insertion site was a predictor of CVC-tip colonisation, and a predictor of BSI. High level of skin colonisation predicts catheter tip colonisation and possibly subsequent infection. Sustained reduction of bacterial growth at the CVC insertion site is therefore indispensable. Male patients are at particular risk for skin colonisation and may be a target population for additional insertion-site care before and during catheterisation. PMID:20532506

Luft, Dirk; Schmoor, Claudia; Wilson, Christine; Widmer, Andreas F; Bertz, Hartmut; Frei, Reno; Heim, Dominik; Dettenkofer, Markus

2010-12-01

266

Survival of children with cancer in Italy, 1989-98. A report from the hospital based registry of the Italian Association of Paediatric Haematology and Oncology (AIEOP).  

PubMed

We describe the survival patterns of 10,791 Italian children (age 0-14) diagnosed with cancer during 1989-1998 and who were included in the hospital-based registry of the Italian Association of Paediatric Haematology and Oncology. Five-year cumulative survival percentages were 76% for lymphoproliferative disorders and 68% for solid tumours. Survival rates in 1994-1998 significantly improved for acute lymphocytic leukaemia (ALL), acute non-lymphocytic leukaemia, Hodgkin's lymphoma and Wilms' tumour. Gender and age were determinants of survival for some specific types of cancer. Girls with ALL and neuroblastoma exhibited a significant advantage (hazard ratio HR 0.72, 0.62-0.83) and disadvantage (HR 0.73, 0.59-0.90) over boys, respectively. Children with a Wilms' tumour diagnosed above age 3 had a worse prognosis than younger children (HR 2.3, 1.4-4.1). The persisting gender-related difference in survival rate for ALL requires understanding as to whether it is attributable to delays in the adoption of more recent therapeutic protocols, while the corresponding findings for Wilms' tumour and neuroblastoma deserve further biological interpretation. PMID:18430564

Pession, Andrea; Dama, Elisa; Rondelli, Roberto; Magnani, Corrado; De Rosa, Marisa; Locatelli, Franco; Fagioli, Franca; Haupt, Riccardo; Jankovic, Momcilo; Terracini, Benedetto; Merletti, Franco; Pastore, Guido

2008-06-01

267

Clinical utility of Aspergillus galactomannan and PCR in bronchoalveolar lavage fluid for the diagnosis of invasive pulmonary aspergillosis in patients with haematological malignancies.  

PubMed

Interpretation of Aspergillus galactomannan (GM) and PCR results in bronchoalveolar lavage (BAL) fluid for the diagnosis of invasive pulmonary aspergillosis (IPA) in patients with haematological malignancies requires clarification. A total of 116 patients underwent BAL for investigation of new lung infiltrates: 40% were neutropenic, 68% and 36% were receiving mould-active antifungal agents and ?-lactam antibiotics. The diagnosis of proven IPA (n=3), probable IPA (n=15), and possible invasive fungal disease (IFD, n=50) was made without inclusion of GM results. BAL GM (at cut-off of 0.8) had lower diagnostic sensitivity for IPA than PCR (61% versus 78%) but higher specificity (93% versus 79%). Both tests had excellent negative predictive values (85-90%), supporting their utility in excluding IPA. The use of BAL GM and PCR results increased the certainty of Aspergillus aetiology in 7 probable IPA cases where fungal hyphae were detected in respiratory samples by microscopy, and upgraded 24 patients from possible IFD to probable IPA. Use of BAL GM and PCR improves the diagnosis of IPA. PMID:24768294

Heng, Siow-Chin; Chen, Sharon C-A; Morrissey, C Orla; Thursky, Karin; Manser, Renee L; De Silva, Harini D; Halliday, Catriona L; Seymour, John F; Nation, Roger L; Kong, David C M; Slavin, Monica A

2014-07-01

268

Novel methicillin-resistant coagulase-negative Staphylococcus clone isolated from patients with haematological diseases at the Blood Bank Centre of Amazon, Brazil  

PubMed Central

Methicillin-resistant Staphylococcus remains a severe public health problem worldwide. This research was intended to identify the presence of methicillin-resistant coagulase-negative staphylococci clones and their staphylococcal cassette chromosome mec (SCCmec)-type isolate from patients with haematologic diseases presenting bacterial infections who were treated at the Blood Bank of the state of Amazonas in Brazil. Phenotypic and genotypic tests, such as SCCmec types and multilocus sequence typing (MLST), were developed to detect and characterise methicillin-resistant isolates. A total of 26 Gram-positive bacteria were isolated, such as: Staphylococcus epidermidis (8/27), Staphylococcus intermedius (4/27) and Staphylococcus aureus (4/27). Ten methicillin-resistant staphylococcal isolates were identified. MLST revealed three different sequence types: S. aureus ST243, S. epidermidis ST2 and a new clone of S. epidermidis, ST365. These findings reinforce the potential of dissemination presented by multi-resistant Staphylococcus and they suggest the introduction of monitoring actions to reduce the spread of pathogenic clonal lineages of S. aureus and S. epidermidis to avoid hospital infections and mortality risks.

Ferreira, Cristina Motta; Naveca, Felipe Gomes; Ferreira, William Antunes; de Oliveira, Cintia Mara Costa; Barbosa, Maria das Gracas Vale

2013-01-01

269

Virulence determinants in vancomycin-resistant Enterococcus faecium vanB: clonal distribution, prevalence and significance of esp and hyl in Australian patients with haematological disorders.  

PubMed

European studies have suggested that the esp gene and other virulence factors have roles in vancomycin-resistant Enterococcus faecium (VREfm) infections. The aim of this study was to examine the relationship between the spectrum of clinical disease and putative virulence factors in vanB VREfm isolates. A multiplex polymerase chain reaction was used to amplify potential virulence genes (asa1, gel E, cylA, esp and hyl) in VREfm isolates obtained from an Australian population of haematology patients. Clonality was assessed by pulsed-field gel electrophoresis (PFGE) and automated ribotyping. Infection, requirement for intensive care unit (ICU) admission and all-cause 30-day mortality were used as clinical indicators of organism virulence. Forty-one VREfm vanB isolates (41 patients; 14 infected and 27 colonised only) were analysed. Thirty-five of these isolates were typed by PFGE, 31 of which were represented by three clusters. The esp gene was identified in 22 of 27 (81.5%) screening and 11 of 14 (78.6%) infection-associated isolates. One isolate was hyl gene positive, and no isolate contained asa1, gel E or cylA genes. VREfm infection was independently associated with host factors (underlying diagnosis of acute myeloid leukaemia, age

Worth, L J; Slavin, M A; Vankerckhoven, V; Goossens, H; Grabsch, E A; Thursky, K A

2008-02-01

270

A comparative study of clinical manifestations, haematological and serological responses after experimental infection with Anaplasma phagocytophilum in two Norwegian sheep breeds  

PubMed Central

Background It has been questioned if the old native Norwegian sheep breed, Old Norse Sheep (also called Norwegian Feral Sheep), normally distributed on coastal areas where ticks are abundant, is more protected against tick-borne infections than other Norwegian breeds due to a continuously high selection pressure on pasture. The aim of the present study was to test this hypothesis in an experimental infection study. Methods Five-months-old lambs of two Norwegian sheep breeds, Norwegian White (NW) sheep and Old Norse (ON) sheep, were experimentally infected with a 16S rRNA genetic variant of Anaplasma phagocytophilum (similar to GenBank accession number M73220). The experiment was repeated for two subsequent years, 2008 and 2009, with the use of 16 lambs of each breed annually. Ten lambs of each breed were inoculated intravenously each year with 0.4 ml A. phagocytophilum-infected blood containing approximately 0.5 × 106 infected neutrophils/ml. Six lambs of each breed were used as uninfected controls. Half of the primary inoculated lambs in each breed were re-challenged with the same infectious dose at nine (2008) and twelve (2009) weeks after the first challenge. The clinical, haematological and serological responses to A. phagocytophilum infection were compared in the two sheep breeds. Results The present study indicates a difference in fever response and infection rate between breeds of Norwegian sheep after experimental infection with A. phagocytophilum. Conclusion Although clinical response seems to be less in ON-lambs compared to NW-lambs, further studies including more animals are needed to evaluate if the ON-breed is more protected against tick-borne infections than other Norwegian breeds.

2011-01-01

271

Comparative effects of using black seed (Nigella sativa), cumin seed (Cuminum cyminum), probiotic or prebiotic on growth performance, blood haematology and serum biochemistry of broiler chicks.  

PubMed

A 42-day trial was conducted to compare the effects of the following seven experimental diets, which varied in black seed, cumin seed, probiotic or prebiotic concentrations, on the broiler chicks: control (no additives), diet BS1 (4 g/kg black seed), diet BS2 (8 g/kg black seed), diet CS1 (4 g/kg cumin seed), diet CS2 (8 g/kg cumin seed), diet Pro (1 g/kg probiotic Primalac(®) ) and diet Pre (2 g/kg prebiotic Fermacto(®) ). A total of 420 1-day-old male broiler chicks, initially weighing an average of 43 g, were distributed into 28 floor pens at a stocking density of 15 birds per pen. At 28 day of age, the body weight in the birds fed diets BS2 , CS2 and Pro was significantly higher than in the control group, but final body weight was not affected. Additionally, the birds fed diets BS2 , Pro and Pre exhibited better feed conversion ratio than control birds from 0 to 42 day of age. Diets BS2 , CS2 and Pro also statistically increased the relative weight of thymus and bursa of Fabricius, whereas only diet Pro decreased the abdominal fat percentage compared with control diet. Regarding the haematological parameters, feeding diet BS2 yielded a significant increase in red blood cell count, haemoglobin concentration and haematocrit percentage compared with control diet. Serum total cholesterol and low-density lipoprotein cholesterol levels in the birds fed diets BS2 , Pro and Pre were also significantly lower than in the birds fed the control diet. Without exception, no diets affected feed intake, internal organs weights, carcass characteristics, antibody titres against Newcastle and influenza viruses and leucocyte subsets. In general, current study showed promising results regarding the use of spice additives as growth and health promoters, especially at higher levels of their incorporation in the diets, which were comparable to the probiotic- or prebiotic-containing diets. PMID:23909469

Alimohamadi, K; Taherpour, K; Ghasemi, H A; Fatahnia, F

2014-06-01

272

An open study of the comparative efficacy and safety of caspofungin and liposomal amphotericin B in treating invasive fungal infections or febrile neutropenia in patients with haematological malignancy.  

PubMed

In a clinical non-trial setting, the efficacy and safety of caspofungin was compared with liposomal amphotericin B for the management of febrile neutropenia or invasive fungal infections in 73 episodes in patients with haematological malignancy. There were fewer episodes of drug toxicity with caspofungin than liposomal amphotericin B (58.3 vs 83.7 %, P=0.02). The favourable response rate for episodes of febrile neutropenia treated with caspofungin or liposomal amphotericin B was similar at 37.5 and 53.8 %, respectively, but more breakthrough fungal infections occurred with caspofungin than with liposomal amphotericin B (33.3 vs 0 %, P<0.05) in these patients who did not receive antifungal prophylaxis. None of four episodes of candidaemia or hepatosplenic candidiasis responded to caspofungin compared with three of four episodes treated with liposomal amphotericin B. Mortality was significantly higher with caspofungin treatment compared with liposomal amphotericin B (6/24 vs 2/49, P=0.01), mainly due to an excess of fungal infections (P=0.04). Caspofungin treatment was a significant independent predictor of mortality [odds ratio=7.6 (95 % confidence interval 1.2-45.5)] when sepsis severity, prolonged neutropenia and length of antifungal therapy were considered in a multiple logistic regression model. In clinical practice, there is a suggestion that caspofungin may not be as effective as liposomal amphotericin B in preventing breakthrough invasive fungal infections in febrile neutropenia or in preventing fungus-related deaths. Because of the potential biases in this observational study, these preliminary findings should be interpreted with caution and clarified with a larger cohort of patients. PMID:17005784

Ellis, Michael; Frampton, Chris; Joseph, Jose; Alizadeh, Hussain; Kristensen, Jorgen; Hauggaard, Anders; Shammas, Fuad

2006-10-01

273

A 30-year epidemiologic survey (1974-2003) of haematological malignancies on the island of Sardinia: temporal changes in incidence and a geographic comparison of incidence rates.  

PubMed

In a previous survey of newly diagnosed haematological malignancies (HMs) in Sardinia from 1974 to 1993, we observed a marked increase in the incidence of many HMs that we chiefly attributed to improvements in case ascertainment. To better define the nature of this increase, we extended the survey by an additional decade (1994-2003), applying the same previously used methods. The incidence of HMs further increased from 1994 to 2003. The incidence rate of total HMs (THMs), standardised to the world population, was 30.15?×?10(5) person-years vs. 21.58 from 1984 to 1993 and 15.26 from 1974 to 1983. The temporal variations in the incidence differed in different HMs and were correlated with the diseases clinical characteristics and the increased availability of diagnostic tools and skills in Sardinia. These observations support the hypothesis that the temporal differences in the incidence rates observed for many HMs in Sardinia over the 30-year survey period were caused by temporal differences in diagnostic efficiency rather than by disease occurrence. An important exception was the increase in non-Hodgkin's lymphoma, which represents a true increase in occurrence, similarly to most Western countries. The incidence rates of HMs already having or reaching stable values in the decade 1994-2003 were similar to those of most Western countries. No significant evidence emerged to suggest that Sardinian particularities influenced the occurrence of HMs. This study demonstrates the extent to which diagnostic efficiency can influence incidence evaluations and emphasises the importance of prolonged observation to determine the validity of incidence rates for both temporal and geographic comparisons. PMID:24464318

Broccia, G; Longinotti, M; Gabbas, A; Porcu, Caterina; Chessa, E; Giannico, Barbara

2014-06-01

274

Long-term follow-up of bone density, general and reproductive health in female survivors after treatment for haematological malignancies.  

PubMed

The purpose of this study was to assess the ovarian function, fertility and bone mineral density in women who previously had treatment for different haematological malignancies (HMs). The overall survival and cure rates of patients with HMs have improved dramatically. The treatment affects fertility and bone density. Fifty-two premenopausal women, from Stockholm region, were included in the study between 1998 and 2002, followed until 2011. The diagnoses were acute lymphoblastic leukaemia (n = 6), acute myeloid leukaemia (n = 10), chronic lymphocytic leukaemia (n = 1), chronic myeloid leukaemia (n = 12), Hodgkin lymphoma (n = 12) and non-Hodgkin lymphoma (n = 11). Before treatment, women without children (43/52), when possible, were offered fertility preservation options. The mean age at diagnosis was 27, at final evaluation 39 yr. Thirty-seven patients received HSCT; 26 allogeneic, 11 autologous. Before allogeneic HSCT, nineteen patients had myeloablative conditioning; seven had reduced-intensity conditioning. Eleven patients got total body irradiation. Eight patients were transplanted with grafts from an HLA-identical sibling donor, while 18 had unrelated donors. All women were in a menopausal state post-therapy. Hormone replacement therapy (HRT) was given, and bone mineral density (BMD) was measured every other year. The serum levels of parathyroid hormone (PTH), free and bound calcium was within normal range. BMD measurements showed a slight increase over time in the spine with a mean of 0.015 g/cm(2) /yr. Four spontaneous pregnancies resulted in two babies and two discontinued pregnancies; two pregnancies were achieved with oocyte donation and surrogacy and one woman adopted a child. HRT sustains BMD in long-term survivors from HMs. This study highlights the importance of HRT and fertility issues in this patient group. PMID:24649942

Naessén, Sabine; Bergström, Ingrid; Ljungman, Per; Landgren, Britt-Marie

2014-08-01

275

Hygienic safety of reusable tap water filters (Germlyser®) with an operating time of 4 or 8 weeks in a haematological oncology transplantation unit  

PubMed Central

Background Microbial safe tap water is crucial for the safety of immunosuppressed patients. Methods To evaluate the suitability of new, reusable point-of-use filters (Germlyser®, Aquafree GmbH, Hamburg, Germany), three variations of a reusable filter with the same filter principle but with different outlets (with and without silver) and inner surface coating of the filter encasements (with and without nano-crystalline silver) were tested. The filter efficacy was monitored over 1, 4 and 8 weeks operating time in a haematological oncology transplantation unit equipped with 18 water outlets (12 taps, 6 showers). Results The filtered water fulfilled the requirements of absence of pathogens over time. From 348 samples, 8 samples (2.3%) exceeded 100 cfu/ml (no sample ? 500 cfu/ml). As no reprocessed filter exhibited 100% filter efficacy in the final quality control after each reprocessing, these contaminations could be explained by retrograde contamination during use. Conclusion As a consequence of the study, the manufacturer recommends changing filters after 4 weeks in high risk areas and after 8 weeks in moderate infectious risk areas, together with routine weekly alcohol-based surface disinfection and additionally in case of visible contamination. The filter efficacy of the 3 filters types did not differ significantly regarding total bacterial counts. Manual reprocessing proved to be insufficient. Using a validated reprocessing in a washer/disinfector with alkaline, acid treatment and thermic disinfection, the filters were effectively reprocessable and now provide tap water meeting the German drinking water regulations as well as the WHO guidelines, including absence of pathogens.

Daeschlein, Georg; Kruger, William H; Selepko, Christian; Rochow, Markus; Dolken, Gottfried; Kramer, Axel

2007-01-01

276

Multicentre surveillance study on feasibility, safety and efficacy of antifungal combination therapy for proven or probable invasive fungal diseases in haematological patients: the SEIFEM real-life combo study.  

PubMed

This multicentre observational study evaluated the feasibility, efficacy and toxicity of antifungal combination therapy (combo) as treatment of proven or probable invasive fungal diseases (IFDs) in patients with haematological malignancies. Between January 2005 and January 2010, 84 cases of IFDs (39 proven and 45 probable) treated with combo were collected in 20 Hematological Italian Centres, in patients who underwent chemotherapy or allogeneic haematopoietic stem cell transplantation for haematological diseases. Median age of patients was 34 years (range 1-73) and 37% had less than 18 years. Acute leukaemia was the most common underlying haematological disease (68/84; 81%). The phase of treatment was as follows: first induction in 21/84 (25%), consolidation phase in 18/84 (21%) and reinduction/salvage in 45/84 (54%). The main site of infection was lung with or without other sites. The principal fungal pathogens were as follows: Aspergillus sp. 68 cases (81%), Candida sp. six cases (8%), Zygomycetes four cases (5%) and Fusarium sp. four cases (5%). The most used combo was caspofungin+voriconazole 35/84 (42%), caspofungin + liposomal amphotericin B (L-AmB) 20/84 (24%) and L-AmB+voriconazole 15/84 (18%). The median duration of combo was 19 days (range 3-180). The overall response rate (ORR) was 73% (61/84 responders) without significant differences between the combo regimens. The most important factor that significantly influenced the response was granulocyte (PMN) recovery (P 0.009). Only one patient discontinued therapy (voriconazole-related neurotoxicity) and 22% experienced mild and reversible adverse events (hypokalaemia, ALT/AST increase and creatinine increase). The IFDs-attributable mortality was 17%. This study indicates that combo was both well tolerated and effective in haematological patients. The most used combo regimens were caspofungin + voriconazole (ORR 80%) and caspofungin + L-AmB (ORR 70%). The ORR was 73% and the mortality IFD related was 17%. PMN recovery during combo predicts a favourable outcome. Clinical Trials Registration: NCT00906633. PMID:24373120

Candoni, A; Caira, M; Cesaro, S; Busca, A; Giacchino, M; Fanci, R; Delia, M; Nosari, A; Bonini, A; Cattaneo, C; Melillo, L; Caramatti, C; Milone, G; Scime', R; Picardi, M; Fanin, R; Pagano, L

2014-06-01

277

Routine application of a novel MLPA-based first-line screening test uncovers clinically relevant copy number aberrations in haematological malignancies undetectable by conventional cytogenetics.  

PubMed

Objective The presence of numerical and/or structural chromosomal abnormalities is a frequent finding in clonal hematopoietic malignant disease, typically diagnosed through routine karyotyping and/or fluorescent in situ hybridization (FISH) analysis. Recently, the application of array comparative genomic hybridization (aCGH) has uncovered many new cryptic genomic copy number imbalances, most of which are now recognized as clinically useful markers of haematological malignancies. In view of the limitations of both FISH and aCGH techniques, in terms of their routine application as a first line screening test, we designed a new multiple ligation-dependent probe amplification (MLPA) probemix for use in addition to classic karyotype analysis. Methods A novel MLPA probemix was developed to interrogate copy number changes involving chromosomal regions: 2p23-24 (MYCN, ALK), 5q32-34 (MIR145A, EBF1, MIR146A), 6q21-27, 7p12.2 (IKZF1), 7q21-36, 8q24.21 (MYC), 9p24 (JAK2 V617F point mutation), 9p21.3 (CDKN2A/2B), 9p13.2 (PAX5), 10q23 (PTEN), 11q22.3 (ATM), 12p13.2 (ETV6), 13q14 (RB1, MIR15A, DLEU2, DLEU1), 17p13.1 (TP53), and 21q22.1 (RUNX1/AML1) and was applied to DNA extracted from 313 consecutive bone marrow patient samples, referred for routine karyotype analysis. Results More than half of the samples originated from newly investigated patients. We discovered clinically relevant genomic aberrations, involving a total of 24 patients (8%) all with a normal karyotype, which would have remained undiagnosed. Discussion Our data clearly indicate that routine application of this MLPA screening panel, as an adjunct to karyotype analysis, provides a sensitive, robust, rapid and low-cost approach for uncovering clinically important genomic abnormalities, which would have otherwise remained undetected. PMID:23928116

Konialis, Christopher; Savola, Suvi; Karapanou, Sophia; Markaki, Aggeliki; Karabela, Maria; Polychronopoulou, Sophia; Ampatzidou, Maria; Voulgarelis, Michael; Viniou, Nora-Athina; Variami, Eleni; Koumarianou, Argyro; Zoi, Katerina; Hagnefelt, Birgitta; Schouten, Jan P; Pangalos, Constantinos

2014-06-01

278

Severe malnutrition with and without HIV-1 infection in hospitalised children in Kampala, Uganda: differences in clinical features, haematological findings and CD4+ cell counts  

PubMed Central

Background The aim of this study was to describe the clinical features, haematological findings and CD4+ and CD8+ cell counts of severely malnourished children in relation to human immunodeficiency virus (HIV) infection. Methods The study was conducted in the paediatric wards of Mulago hospital, which is Uganda's national referral and teaching hospital. We studied 315 severely malnourished children (presence of oedema and/or weight-for-height: z-score < -3) and have presented our findings. At admission, the CD4+ and CD8+ cells were measured by the flow cytometry and HIV serology was confirmed by Enzyme linked Immunoassay for children >18 months of age, and RNA PCR was performed for those ?18 months. Complete blood count, including differential counts, was determined using a Beckman Coulter counter. Results Among the 315 children, 119 (38%) were female; the median age of these children was 17 months (Interquartile range 12–24 months), and no difference was observed in the HIV status with regard to gender or age. The children showed a high prevalence of infections: pneumonia (68%), diarrhoea (38%), urinary tract infection (26%) and bacteraemia (18%), with no significant difference with regard to the HIV status (HIV-positive versus HIV-negative children). However, the HIV-positive children were more likely to have persistent diarrhoea than the HIV-uninfected severely malnourished children (odds ratio (OR) 2.0, 95% confidence interval (CI) 1.2–3.6). When compared with the HIV-negative children, the HIV-positive children showed a significantly lower median white blood cell count (10700 versus 8700) and lymphocyte count (4033 versus 2687). The CD4+ cell percentages were more likely to be lower in children with non-oedematous malnutrition than in those with oedematous malnutrition even after controlling for the HIV infection. The novel observation of this study is that the CD4+ percentages in both HIV-positive and HIV-negative children without oedema were lower that those in children with oedema. These observations appear to imply that the development of oedema requires a certain degree of immunocompetence, which is an interesting clue to the pathophysiology of oedema in severe malnutrition.

Bachou, Hanifa; Tylleskar, Thorkild; Downing, Robert; Tumwine, James K

2006-01-01

279

Highly sensitive and specific detection of P-glycoprotein function for haematological and solid tumour cells using a novel nucleic acid stain.  

PubMed Central

Progress in our understanding of the contribution of P-glycoprotein (P-gp)-mediated resistance to chemotherapy failure in haematological as well as solid tumours has been hampered by the lack of highly sensitive, reliable methods for the detection of P-gp function in fresh human tumour cells. The present study identifies the novel nucleic acid stain SYTO16 as a highly sensitive and specific dye to assess P-gp function. The effect of P-gp is expressed here as the ratio of dye fluorescence (RF) from cells incubated with dye with or without 2 microM of the P-gp inhibitor PSC 833. Using flow cytometric analysis, an RF of 0.9 was found for SYTO16 in the KB3-1 (P-gp-) and 1.6 in KB8 (P-gp+) cells. Three types of patients' cells were studied: (1) in haematopoietic CD34+ cells, which are known to express P-gp, the RF was 6.0 for SYTO16 compared with 2.5 for rhodamine 123 and 1.3 for daunorubicin (mean of five individuals); (2) in acute myeloid leukaemia cells, the RF for SYTO16 was 1.0 in P-gp- and 4.5 in P-gp+ samples; (3) for the first time, we have quantitated P-gp function in fresh human solid tumour (sarcoma) cells. We found, in a P-gp+ leiomyosarcoma, an RF of 16 for SYTO16 and 2.7 for daunorubicin. This means that complete inhibition of P-gp function in these sarcoma cells would lead to an increase of daunorubicin accumulation with 170% compared with 30% in the CD34+ cells. Next, we showed that SYTO16 could be fixed in nuclei by 3.6% formaldehyde treatment, allowing quantification of the nuclear fluorescence on cytospins by laser scanning microscopy. In conclusion, SYTO16 proved to have a combination of favourable properties: it can be excited at 488 nm and has large fluorescence enhancement upon binding to nucleic acids, allowing the use of low, nontoxic (< 10 nM) concentrations. Because the RF for SYTO16 is much higher than for daunorubicin, it can be applied for the determination of P-gp function in relatively small numbers of low-P-gp-expressing tumour cells by laser scanning microscopy. Individual sarcomas were found to have high P-gp function compared with CD34+ cells. This assay may be used to select patients for P-gp modulation protocols. Images Figure 2 Figure 4

Broxterman, H. J.; Schuurhuis, G. J.; Lankelma, J.; Oberink, J. W.; Eekman, C. A.; Claessen, A. M.; Hoekman, K.; Poot, M.; Pinedo, H. M.

1997-01-01

280

Haematological changes in HIV infection.  

PubMed

HIV infection is associated with a wide range of hematological abnormalities. The peripheral blood findings and the morphological abnormalities in the bone marrow can simulate myelodysplastic syndrome, myeloproliferative disorders, and T cell lymphoma. We studied the peripheral blood smear and bone marrow findings of 42 patients with HIV infection over a 3-year period with the aim of recognising the morphological findings sufficiently characteristic of HV infection. The salient peripheral blood smear findilngs were anemia, bicytopenia and pancytopenia. The bone marrow revealed trilineage dysplasia, plasma cells and eosinophils, increased megakaryocytes, increased iron and reticulin fibrosis. In two cases the bone marrow revealed granulomata. PMID:15022904

Sitalakshmi, S; Srikrishna, Anuradha; Damodar, Prema

2003-04-01

281

Characterization of haematological parameters with bortezomib-melphalan-prednisone versus melphalan-prednisone in newly diagnosed myeloma, with evaluation of long-term outcomes and risk of thromboembolic events with use of erythropoiesis-stimulating agents: analysis of the VISTA trial.  

PubMed

Although haematological toxicities, such as anaemia, are common in multiple myeloma (MM), no clear consensus exists on the use and impact of erythropoiesis-stimulating agents (ESA) on outcomes in MM. This analysis characterizes haematological toxicities and associated interventions in the phase III VISTA (Velcade(®) as Initial Standard Therapy in Multiple Myeloma: Assessment with Melphalan and Prednisone) study of bortezomib plus melphalan/prednisone (VMP, n?=?344) versus MP (n?=?338) in previously untreated MM patients ineligible for high-dose therapy, and evaluates the impact of ESA use or red-blood-cell (RBC) transfusions on outcomes and thromboembolic risk. Incidence of haematological toxicities was similar with VMP and MP; similar rates of interventions and associated complications (e.g. bleeding, febrile neutropenia) were observed. Two hundred thirty three patients received ESA; 204 had RBC transfusions. Frequency of thromboembolic events was low and not affected by ESA use. Median time-to progression (TTP) was similar between ESA/non-ESA [hazard ratio: 1·03 (95% confidence interval 0·76-1·39); P?=?0·8478] in both arms (VMP: 19·9/not reached; MP: 15·0/17·5?months). Three-year overall survival (OS) rates were similar between ESA/non-ESA in each arm. Patients receiving RBC transfusions had significantly shorter OS (P?haematological toxicity. Concomitant ESA use with VMP/MP in previously untreated MM patients did not adversely affect TTP or OS, or increase thromboembolic risk. However, RBC transfusion was associated with significantly shorter survival. PMID:21375521

Richardson, Paul; Schlag, Rudolf; Khuageva, Nuriet; Dimopoulos, Meletios; Shpilberg, Ofer; Kropff, Martin; Vekemans, Marie-Christiane; Petrucci, Maria Teresa; Rossiev, Viktor; Hou, Jian; Robak, Tadeusz; Mateos, Maria-Victoria; Anderson, Kenneth; Esseltine, Dixie-Lee; Cakana, Andrew; Liu, Kevin; Deraedt, William; van de Velde, Helgi; San Miguel, Jesús F

2011-04-01

282

Outcomes of hematopoietic stem cell transplantation in primary immunodeficiency: a report from the Australian and New Zealand Children's Haematology Oncology Group and the Australasian Bone Marrow Transplant Recipient Registry.  

PubMed

We performed a retrospective analysis on the outcomes of 135 hematopoietic stem cell transplantations (HSCTs) for primary immunodeficiency disorders in Australian and New Zealand Children's Haematology Oncology Group transplantation centers between 1992 and 2008. The most common indications for HSCT were severe combined immunodeficiency, Wiskott-Aldrich syndrome, and chronic granulomatous disease. Five-year overall survival (OS) was 72% for the entire cohort. Disease-specific 5-year OS was 70% for severe combined immunodeficiency, 81% for Wiskott-Aldrich syndrome, and 69% for chronic granulomatous disease. Transplantation-related mortality (TRM) was 10% at day +100. TRM and OS were equivalent in recipients of related and unrelated donor transplants. Source of stem cells had no impact on TRM or OS with outcomes following unrelated umbilical cord blood similar to unrelated bone marrow. The presence of interstitial pneumonitis, active cytomegalovirus infection, or veno-occlusive disease were all independent variables that significantly decreased OS. This large series supports the use of HSCT as curative therapy for a range of primary immunodeficiency disorders, demonstrating excellent survival after both related and unrelated donor transplantation. PMID:23228588

Mitchell, Richard; Nivison-Smith, Ian; Anazodo, Antoinette; Tiedemann, Karin; Shaw, Peter; Teague, Lochie; Fraser, Chris; Carter, Tina; Tapp, Heather; Alvaro, Frank; O'Brien, Tracey A

2013-03-01

283

Long-term outcome and prognostic factors of unrelated cord blood transplantation in children with haematological malignancies: a retrospective study using the Spanish Working Party for BMT in Children (GETMON) database.  

PubMed

Outcomes of unrelated cord blood transplants (UCBT) were assessed in 172 consecutive children, median age 5 years (range: 0.5-18), with haematological malignancies treated at nine Spanish hospitals between February 1996 and April 2009. Data were collected from the Spanish Working Party for Blood and Marrow Transplantation in Children (GETMON) database. ALL was diagnosed in 125 patients, AML in 43 and myelodysplastic syndrome in 4. Myeloid engraftment (ANC?0.5 × 10(9)/L) occurred in 87.2% at a median of 22 days and was associated with the total nucleated cell (TNC) dose infused and use of a TT-containing conditioning regimen. Cumulative incidence of relapse was 20% at 1 year post transplant and 29% at 3 years, being higher in patients with a diagnosis of ALL, very high risk disease and GVHD grades 0-1. Cumulative incidence of non-relapse mortality (NRM) was 19% at 100 days post transplant and 39% at 1 year. BU-FLU-TT-ATG-conditioned patients had lower NRM. Disease-free survival (DFS) was 40% at 2 years post transplant (for patients transplanted since 2006). On multivariate analysis, TNC dose infused, AML and BU-FLU-TT-ATG-conditioning regimen increased the probability of DFS. It is of paramount importance to select cord blood units with the highest cell dose. As the BU-FLU-TT-ATG-conditioning regimen was associated with better DFS owing to lower NRM, further prospective studies testing this regimen are warranted. PMID:24614843

Díaz de Heredia, C; González, M; Verdeguer, A; Elorza, I; Rodriguez, A; Martinez, A; Pérez, J M; Badell, I; Gonzalez, M E; Olivé, T; Fernández, J M; Maldonado, M S; Díaz, M A; Sánchez de Toledo, J

2014-06-01

284

Evaluation of growth performance, serum biochemistry and haematological parameters on broiler birds fed with raw and processed samples of Entada scandens, Canavalia gladiata and Canavalia ensiformis seed meal as an alternative protein source.  

PubMed

The experiment was carried out to investigate the inclusion of underutilised legumes, Entada scandens, Canavalia gladiata and Canavalia ensiformis, seed meal in soybean-based diet in broilers. The utilisation of these wild legumes is limited by the presence of antinutrient compounds. Processing methods like soaking followed by autoclaving in sodium bicarbonate solution in E. scandens and C. gladiata and soaking followed by autoclaving in ash solution in C. ensiformis were adopted. The proximate composition of raw and processed samples of E. scandens, C. gladiata and C. ensiformis were determined. The protein content was enhanced in processed sample of E. scandens (46 %) and C. ensiformis (16 %). This processing method had reduced the maximum number of antinutrients such as tannins (10-100 %), trypsin inhibitor activity (99 %), chymotrypsin inhibitor activity (72-100 %), canavanine (60-62 %), amylase inhibitor activity (73-100 %), saponins (78-92 %), phytic acid (19-40 %) and lectins. Hence, the raw samples at 15 % and processed samples at 15 and 30 % were replaced with soybean protein in commercial broiler diet respectively. Birds fed with 30 % processed samples of E. scandens, C. gladiata and C. ensiformis showed significantly similar results of growth performance, carcass characteristics, organ weight, haematological parameters and serum biochemical parameters (cholesterol, protein, bilirubin, albumin, globulin and liver and kidney function parameters) without any adverse effects after 42 days of supplementation. The proper utilisation of these underutilised legumes may act as an alternative protein ingredient in poultry diets. PMID:23076820

Sasipriya, Gopalakrishnan; Siddhuraju, Perumal

2013-03-01

285

The effect of oyster mushroom ?-1.3/1.6-D-glucan and oxytetracycline antibiotic on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in common carp (Cyprinus carpio L.).  

PubMed

The aim of the study was to evaluate the effect of micronized ?-1.3/1.6-D-glucan (BG) derived from the oyster mushroom Pleurotus ostreatus Hiratake and tetracycline antibiotic oxytetracycline (OTC) on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in tissues of one- to two-year-old common carp (Cyprinus carpio L.). The fish tested were divided into five experimental groups and one control. Carp in the control group were fed commercial carp feed pellets. Fish in the five experimental groups were fed the same pellets supplemented with either OTC, a combination of OTC and BG, or BG as follows: 75 mg oxytetracycline kg(-1) bw (OTC group), 75 mg oxytetracycline kg(-1) bw and 0.5% ?-glucan (OTC + 0.5% BG group), 75 mg oxytetracycline kg(-1) bw and 2.0% ?-glucan (OTC + 2.0% BG group), 0.5% ?-glucan (0.5% BG group), and 2.0% ?-glucan (2.0% BG group). OTC- and BG-supplemented diets and the control diet were administered to experimental and control carp for 50 days (i.e. samplings 1-3, the exposure period); for the following 14 days, fish were fed only control feed pellets with no OTC or BG supplementation (i.e. sampling 4, the recovery period). Blood and tissue samples were collected both during, and at the end of the study. No significant changes in biometrical indices (i.e. total length, standard length, total weight, hepatosomatic and spleen somatic index, and Fulton's condition factor) were found in experimental carp compared to control in any sampling. In haematological indices, significant changes were found only in sampling 2, in which shifts in PCV (P < 0.01), Hb (P < 0.01), and WBC (P < 0.01), and in the counts of lymphocytes (P < 0.01), monocytes (P < 0.01), and neutrophil granulocytes-segments (P < 0.05) were revealed. As for biochemical profiling, plasma concentrations of glucose, albumins, cholesterol, natrium, and chlorides (all P < 0.01), and total proteins, lactate, phosphorus, and potassium (all P < 0.05) as well as the catalytic activity of ALP (P < 0.05) were altered in common carp. A significant change in induced (opsonizedzymosan particles, OZP) chemiluminescence (P < 0.05) in sampling 3 and no shifts in serum immunoglobulins concentration were found in the immunological analysis. Histopathological examination of skin, gills, liver, spleen, and cranial and caudal kidneys revealed no obvious specific changes in any tissue analysed. The use of ?-glucans in clinically healthy aquaculture remains an issue. Nevertheless, their use in breeding endangered by stress stimuli, infectious disease, or adverse environmental factors is defensible. PMID:24041844

Dobšíková, Radka; Blahová, Jana; Mikulíková, Ivana; Modrá, Helena; Prášková, Eva; Svobodová, Zde?ka; Skori?, Mišo; Jarkovský, Ji?í; Siwicki, Andrzej-Krzysztof

2013-12-01

286

Fertility and sexual function in long-term survivors of haematological malignancy: using patient-reported outcome measures to assess a neglected area of need in the late effects clinic.  

PubMed

Problems of sexual function and fertility in long-term survivors (?5 years) of haematological malignancy are often neglected in clinic. Our centre carried out a questionnaire study in this population addressing patient-perceived fertility and sexual function. 718 patients responded (56% of those invited; 39% Hodgkin, 45% non-Hodgkin lymphoma, 16% acute leukaemia). Respondent women were more likely to remain childless than a normal control population. Self-reported infertility was more likely in men than women [odds ratio (OR) 1·77, P = 0·001]. Myeloablative therapy increased the likelihood of childlessness (OR 2·48, P = 0·004). Few attended fertility support services (12%). 24% of men banked sperm and 29% of these used the sample, of which 46% resulted in successful pregnancy. Fertility clinic attendance and sperm storage was more likely post-1990 (OR 4·05, P < 0·001; OR 5·05, P < 0·001 respectively). Reporting a negative impact of cancer on sexual function was more common in women than men (OR 2·20, P < 0·001), and increased with current age and age at diagnosis (by 3-4% per year, P ? 0·001) but decreased with longer follow-up (by 2%/year, P = 0·005). Patients on anti-depressants and those reporting cancer-related body change/appearance concerns more frequently reported a negative impact (P < 0·04 and P < 0·03 respectively). These self-reported outcomes confirm literature findings, suggest improvement over time, but highlight a need for involvement of support services. PMID:24236665

Greaves, Paul; Sarker, Shah-Jalal; Chowdhury, Kashfia; Johnson, Rachel; Matthews, Janet; Matthews, Rebecca; Smith, Matthew; Korszun, Ania; Gribben, John G; Lister, T Andrew

2014-02-01

287

The influence of Sacoglottis gabonensis stem bark extract and its isolate bergenin, Nigerian alcoholic beverage additives, on the metabolic and haematological side effects of 2,4-dinitrophenyl hydrazine-induced tissue damage.  

PubMed

This study was designed to study the influence of Sacoglottis gabonensis stem bark extract on the metabolic and cytotoxic side effects of 2,4-dinitrophenyl hydrazine (2,4-DNPH) on the brain and blood using male weaving rats as the experimental model. This was after the effect of the bark extract and bergenin, its isolate, on membrane lipid peroxidation and tissue natural antioxidant defences was reported. Lipid peroxidation was induced experimentally with a single intraperitoneal phenylhydrazine (2,4-DNPH) administration at the end of 3 days exposure to the bark extract or bergenin in drinking water. Three hours later, the brain, liver and red blood cells of the experimental animals were analysed for glucose level and the blood was analysed for selected key indices of oxidative stress: red blood cell (RBC) count haemoglobin (Hb), packed cell volume (PCV) and white blood cell (WBC) count (total and differential). The bark extract exhibited a protective action on brain glucose, significantly inhibiting the glucose-depleting action of both 2,4-DNPH and ethanol. It also inhibited the lowering action of DNPH and ethanol on PCV, RBC and Hb concentration of rat blood, but inhibited proliferation of white blood cells (total and differential). The data on the effect of bergenin, on the side effects of 2,4-DNPH experimental lipid peroxidation and on ethanol followed an essentially similar trend to those of the bark extract on brain glucose. Bergenin, similar to the bark extract, exerted a protective action on the brain tissue, though to a lesser extent, against the oxidants, 2,4-DNPH and ethanol. It is evident that aqueous ethanol extract of S. gabonensis stem bark has biological antioxidant properties against 2,4-DNPH and ethanol-induced tissue damage exerting its action on the haematological and metabolic side effects of the oxidants. By virtue of its essentially similar activity under the same conditions, bergenin appears to be the phytochemical constituent that is largely responsible for the observed action of the bark extract. PMID:14567070

Maduka, H C; Okoye, Z S; Eje, A

2002-12-01

288

British Committee for Standards in Haematology  

Microsoft Academic Search

Summary Unselected coagulation testing is widely practiced in the process of assessing bleeding risk prior to surgery. This may delay surgery inappropriately and cause unnecessary concern in patients who are found to have 'abnormal' tests. In addition it is associated with a significant cost. This systematic review was performed to determine whether patient bleeding history and unselected coagulation testing predict

Y. L. Chee; J. C. Crawford; H. G. Watson; M. Greaves

289

Adverse haematological outcome and environmental lead poisoning  

Microsoft Academic Search

The South-eastern Area (SA) of the Municipality of La Spezia (Liguria Region, Italy) is characterised by a heavy environmental lead (Pb) contamination, chiefly due to the emissions of a Pb-processing plant in operation since 1930. In order to assess the risk of Pb poisoning of residents of SA, and to estimate the degree of association between the blood Pb level

Vincenzo Fontana; Roberta Baldi; Michela Franchini; Paola Gridelli; Roberto Neri; Franco Palmieri; Riccardo Puntoni; Umberto Ricco; Stefano Parodi

2004-01-01

290

Not so benign haematology: anaemia of the elderly  

PubMed Central

Summary Developed countries, such as the United Kingdom, are experiencing a change in demographics resulting in the largest proportion of adults over 65 years of age that our health systems have ever experienced. As such, haematologists must be prepared to evaluate and treat anaemia in a more complicated patient population, but sufficient evidence-based guidelines are lacking. Critical next steps that must be taken to ensure the best care of this population include the determination of appropriate haemoglobin concentrations for older adults in light of age, gender, race, and comorbidities; the development of interventional trials that address physical performance outcomes in addition to haemoglobin targets; and translational studies which address the molecular pathogenesis of anaemia in older adults with the most advanced scientific approaches.

Merchant, Akil A.; Roy, Cindy N.

2011-01-01

291

Cutaneous mast cell tumor (Mastocytoma): Cyto- histopathological and haematological investigations  

PubMed Central

Cutaneous mast cell tumours (MCTs) are the most common skin tumours in dogs. Due to the prevalence of canine MCTs and the variable biologic behavior of this disease, accurate prognostication and a thorough understanding of MCT biology are critical for the treatment of this disease. A cytologic diagnosis of mast cell tumor with evidence of prior hemorrhage was made, and the masses were surgically removed. Cytological evaluation of fine-needle aspirates from the cutaneous mass from the axillary comprised many well-differentiated, highly granulated mast cells with moderate numbers of eosinophils. Nuclei were varied in size and shape with high nuclear’to’cytoplasmic ratio, prominent nucleoli, marked atypical and mitotic figures. Microscopically, mass consisted of sheets of neoplastic round cells that formed nonencapsulated nodules in the dermis and infiltrated into the adjacent dermal collagen, and also there was diffuse subcutis invasion of round to pleomorphic tumor cells. Tumor cells had moderate to abundant cytoplasm, round to ovoid nuclei with scattered chromatin, and mitotic figures. In this tumor, cytoplasmic granules showed atypical metachromasia. In addition, eosinophils were scattered among the mast cells at the periphery of the nodules. The presence of eosinophils and the observation, at high magnification, of cells with cytoplasmic metachromatic granules. Invasion of the deep subcutaneous fat or cutaneous muscles were a common feature of grade III tumour. Finally, a diagnosis of grade III cutaneous mast cell tumor was made. Virtual slides The virtual slide(s) of this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4755249151157024.

2014-01-01

292

Effects of electroplating effluent on haematological parameters of Oreochromis mossambicus  

NASA Astrophysics Data System (ADS)

Fresh water fish, Oreochromis mossambicus was exposed to Electroplating effluent with a view to check the hematological changes as a function of time and effluent concentration. Increase in time and concentration always produced a declining value of RBC, Hb, Ht and WBC. This level is statistically significant in most of the test concentrations and in the experimental duration. The calculated MCV, MCH and MCHC also exhibit the same trend in all the selected sub lethal concentrations of the effluent, which is also found to be time and dose dependent.

Navaraj, P. S.; Kumaraguru, A. K.

2003-05-01

293

Trial of Haploidentical Stem Cell Transplantation for Haematological Cancers  

ClinicalTrials.gov

Hodgkin's Lymphoma; Non-Hodgkin's Lymphoma; Acute Myeloid Leukaemia; Acute Lymphoblastic Leukaemia; Myelodysplastic Syndrome; Chronic Myeloid Leukaemia; Chronic Lymphocytic Leukaemia; Acquired Bone Marrow Failure Syndromes

2012-05-10

294

Neonatal haematology in Zimbabwe. I: The platelet parameters.  

PubMed

Platelet parameters were determined in 600 full term healthy Zimbabwean neonates using a Coulter Counter Model S Plus. The mean +/- s.d. values obtained with ranges were: Platelet count 235 +/- 67 (range 100-410) x 10(9) l-1; Mean Platelet Volume (MPV) 9.5 +/- 1.0 (range 7.1-13.9) fl; Plateletcrit (PCT) 0.269 +/- 0.058 (range 0.142-0.524) and Platelet Distribution Width (PDW) 16.8 +/- 1.0 (range 12.2-19.8). Although platelet counts in this study were comparable to those established by others in both Black and Caucasian infants, the female Zimbabwean neonates had significantly higher counts than the males (p < 0.001). An inverse relationship between the number of circulating platelets and their MPV was also observed. Further studies are needed to delineate the range of normality of these platelet measurements in the African neonate so that abnormal cases can be adequately screened, investigated and appropriately treated. PMID:7954714

Mukiibi, J M; Nkrumah, F K; Kaur, M; Akino, V; Nhembe, M

1994-04-01

295

Haematological Parameters and Altered Erythrocyte Metabolism in Anaemic Dogs  

Microsoft Academic Search

In previous studies, an increase in the activity of pyruvate kinase (PK) and glucose-6-phosphate dehydrogenase (G6PDH), an increase in the concentration of 2,3-diphosphoglycerate (2,3DPG), and alterations in osmotic fragility were found in dogs with haemolytic anaemia. These changes were mainly caused by the presence of immature red cells found in regenerative anaemias. In the present study, the same parameters were

S Paltrinieri; S Comazzi; F Agnes

2000-01-01

296

Haematological findings in pregnant, postparturient and nursing mares  

Microsoft Academic Search

Venous blood samples were collected from 16 Thoroughbred and Quarter Horse mares during late pregnancy (approximately one month before parturition), approximately 6 h after parturition, one month after parturition and 4 months after parturition when the foals were weaned. There were no significant differences over time for haematocrit, mean cell volume, mean cell haemoglobin concentration, platelet count or mean platelet

J. W. Harvey; R. L. Asquith; M. G. Patel; J. Kivipelto; C. L. Chen; E. A. Ott

1994-01-01

297

Study on biological variability of haematological components in dogs  

Microsoft Academic Search

The aim of the present study was to estimate the between-dog, within-dog and analytical components of variance for red blood\\u000a cell (RBC) concentration, white blood cell (WBC) concentration, haematocrit (HT) and haemoglobin (HGB) in clinically healthy\\u000a dogs and to use these estimates to calculate the critical difference for significance between serial results, to assess the\\u000a utility of the conventional population-based

A. L. Jensen; L. Iversen; T. K. Petersen

1998-01-01

298

Microscopic image analysis using mathematical morphology: Application to haematological cytology  

Microsoft Academic Search

The mathematical morphology is a non-linear image processing approach which is based on the application of lattice theory to spatial structures. This technique is proven to be a very powerful tool in microscopic image analysis. In this chapter, we present the architecture of a system of quantitative microscopy which yields an integrated platform for the morphological image analysis, pattern recognition,

Jesús Angulo; Georges Flandrin

299

Metastatic haematological malignancy presenting as a sellar mass.  

PubMed

A man in his 60s with a history of multiple myeloma diagnosed 6 months previously was referred with a diagnosis of a sellar mass. He reported a 3-month history of headaches associated with double vision which appeared to improve with corticosteroid treatment. An MRI scan revealed a 3.7 cm × 3 cm × 2.6 cm sellar mass with erosion into the sphenoid sinus and extending to the right cavernous sinus. Pituitary function tests were normal apart from hypogonadotrophic hypogonadism. A diagnosis of plasmacytoma was considered and so transphenoidal biopsy of the sellar lesion was organised, which revealed sheets of mature appearing plasma cells, staining exclusively for ?-light chain immunoglobulins. He was to be started on chemotherapy for the myeloma and radiotherapy for the plasmacytoma. The patient's clinical course was complicated by community-acquired pneumonia and renal failure resulting in his death a month after the diagnosis of plasmacytoma. PMID:23175000

Udiawar, Maneesh; Bejnariu, Christina; Davies, Stephen

2012-01-01

300

Cyclic nucleotide phosphodiesterases as targets for treatment of haematological malignancies  

PubMed Central

The cAMP signalling pathway has emerged as a key regulator of haematopoietic cell proliferation, differentiation and apoptosis. In parallel, general understanding of the biology of cyclic nucleotide PDEs (phosphodiesterases) has advanced considerably, revealing the remarkable complexity of this enzyme system that regulates the amplitude, kinetics and location of intracellular cAMP-mediated signalling. The development of therapeutic inhibitors of specific PDE gene families has resulted in a growing appreciation of the potential therapeutic application of PDE inhibitors to the treatment of immune-mediated illnesses and haematopoietic malignancies. This review summarizes the expression and function of PDEs in normal haematopoietic cells and the evidence that family-specific inhibitors will be therapeutically useful in myeloid and lymphoid malignancies.

Lerner, Adam; Epstein, Paul M.

2005-01-01

301

Overview of Histone Deacetylase Inhibitors in Haematological Malignancies  

PubMed Central

Histone deacetylase inhibitors (HDACi) can induce hyperacetylation of both histone and non-histone target resulting in epigenetic reprogramming and altered activity, stability and localisation of non-histone proteins to ultimately mediate diverse biological effects on cancer cells and their microenvironment. Clinical trials have demonstrated single agent HDACi to have activity in hematological malignancies, in particular T-cell lymphoma and Hodgkin lymphoma. Combination strategies with standard therapies based on pre-clinical data are being employed with significant success due to their excellent side effect profile. Correlative studies will provide valuable information on the sub-groups of patients more likely to respond or be resistant to HDACi therapy, while long-term monitoring for toxicities is also needed.

Bishton, Mark J.; Johnstone, Ricky W.; Dickinson, Michael; Harrison, Simon; Prince, H. Miles

2010-01-01

302

Comparison of haematological parameters determined by the Sysmex KX - 2IN automated haematology analyzer and the manual counts  

Microsoft Academic Search

BACKGROUND: This study was designed to determine the correlation between heamatological parameters by Sysmex KX-21N automated hematology analyzer with the manual methods. METHOD: Sixty (60) subjects were randomly selected from both apparently healthy subjects and those who have different blood disorders from the University of Teaching Hospital (UNTH), Ituku-Ozalla, Enugu, Enugu State, Nigeria. Three (3)mls of venous blood sample was

Samuel O Ike; Thomas Nubila; Ernest O Ukaejiofo; Imelda N Nubila; Elvis N Shu; Ifeyinwa Ezema

2010-01-01

303

Detection of EPO doping and blood doping: the haematological module of the Athlete Biological Passport.  

PubMed

The increase of the body's capacity to transport oxygen is a prime target for doping athletes in all endurance sports. For this pupose, blood transfusions or erythropoiesis stimulating agents (ESA), such as erythropoietin, NESP, and CERA are used. As direct detection of such manipulations is difficult, biomarkers that are connected to the haematopoietic system (haemoglobin concentration, reticulocytes) are monitored over time (Athlete Biological Passport (ABP)) and analyzed using mathematical models to identify patterns suspicious of doping. With this information, athletes can either be sanctioned directly based on their profile or targeted with conventional doping tests. Key issues for the appropriate use of the ABP are correct targeting and use of all available information (e.g. whereabouts, cross sectional population data) in a forensic manner. Future developments of the passport include the correction of all concentration-based variables for shifts in plasma volume, which might considerably increase sensitivity. New passport markers from the genomic, proteomic, and metabolomic level might add further information, but need to be validated before integration into the passport procedure. A first assessment of blood data of federations that have implemented the passport show encouraging signs of a decreased blood-doping prevalence in their athletes, which adds scientific credibility to this innovative concept in the fight against ESA- and blood doping. PMID:22374784

Schumacher, Yorck Olaf; Saugy, Martial; Pottgiesser, Torben; Robinson, Neil

2012-11-01

304

Desmopressin in treatment of haematological disorders and in prevention of surgical bleeding.  

PubMed

Stimulation with the vasopressin analogue desmopressin (DDAVP) of extrarenal arginine vasopressin (AVP) V2-receptors in endothelial cells and possible in platelets increases the circulating levels of coagulation factor VIII (FVIII), von Willebrand factor (VWF) and tissue plasminogen activator (t-PA). The purpose of this paper is to provide an updated review of current information on the efficacy and safety of DDAVP in the treatment of haemophilia, von Willebrand disease (VWD), uremia, liver cirrhosis, and in congenital or drug-induced platelet dysfunction - under surgical or non-surgical conditions. In summary, desmopressin is an effective haemostatic drug that when administered i.v., s.c. or intranasally increases plasma levels of FVIII and VWF 2-6 times and improves platelet function. It has a proven haemostatic efficacy in mild haemophilia A and VWD as well as in uremia, liver cirrhosis and in congenital and acquired, drug induced platelet dysfunction. Desmopressin has few side effects but observation is advised in small children and elderly. PMID:24703870

Svensson, Peter J; Bergqvist, Peter B F; Juul, Kristian Vinter; Berntorp, Erik

2014-05-01

305

Special considerations for haematology patients in relation to end-of-life care: Australian findings.  

PubMed

Recent hematology clinical guidelines recommend that palliative care specialists should have central roles in hemato-oncology teams. However, the available research evidence indicates there are presently significant obstacles to the integration of palliative care in hematology. The following discussion presents findings from an Australian study designed to address the problems associated with lack of referral of hematology patients to the palliative system through the development of a best-practice model for end-of-life care for these diagnostic groups. The preliminary step in the development of such a model is to document the factors that denote the special characteristics of the end-of-life stage of hematological conditions and their treatments. This article presents the list of special considerations from a nursing perspective, including issues associated with the high-tech nature of treatments, the speed of change to a terminal event, the need for blood products and possibility of catastrophic bleeds, the therapeutic optimism based on a myriad of treatment options and the clinical indices of the terminal trajectory. The nursing insights provide an important foundation for building a practical, patient-centred model for terminal care in hematology. PMID:17371426

McGrath, P; Holewa, H

2007-03-01

306

Observing a fictitious stressful event: haematological changes, including circulating leukocyte activation.  

PubMed

The aim of this study was to assess the effect of watching a psychological stressful event on the activation of leukocytes in healthy human volunteers. Blood samples were obtained from 32 healthy male and female subjects aged between 20 and 26 years before, during and after either watching an 83-minute horror film that none of the subjects had previously seen (The Texas Chainsaw Massacre, 1974) or by sitting quietly in a room (control group). Total differential cell counts, leukocyte activation as measured by the nitroblue tetrazolium (NBT) test, heart rate and blood pressure (BP) measurements were taken at defined time points. There were significant increases in peripheral circulating leukocytes, the number of activated circulating leukocytes, haemoglobin (Hb) concentration and haematocrit (Hct) in response to the stressor. These were accompanied by significant increases in heart rate, systolic and diastolic BP (P<0.05 from baseline). This is the first reported study on the effects of observing a psychologically stressful, albeit fictitious event on circulating leukocyte numbers and the state of leukocyte activation as determined by the nitrotetrazolium test. PMID:12637206

Mian, Rubina; Shelton-Rayner, Graham; Harkin, Brendan; Williams, Paul

2003-03-01

307

Automated analysis of bone marrow aspirates from dogs with haematological disorders.  

PubMed

Automated analysis of bone marrow (BM) aspirates is a useful 'pre-microscopical' screen to identify hypocellular samples and those with potentially abnormal cells. In order to determine whether automated analysis could also be used to identify haemopoietic abnormalities, EDTA-anticoagulated BM aspirates from 43 dogs were analysed using the Advia 2120 instrument. Corresponding Wright-stained BM smears were evaluated microscopically to determine smear quality, cell composition and 500-cell differential counts, and correlation to automated analysis parameters was computed. Leucocyte cytograms generated by the automated analyzer were scrutinized and compared with those of 'normal' BM. Twenty-three neoplastic and 20 non-neoplastic samples were analysed, including samples from 10 cases of acute myeloid leukaemia, four cases of acute lymphocytic leukaemia, four cases of chronic lymphocytic leukaemia, one case of chronic neutrophilic leukaemia, three cases of multiple myeloma, one case of myelodysplastic syndrome, five cases of non-regenerative immune-mediated haemolytic anaemia, one case of immune-mediated neutropenia, three cases of immune-mediated thrombocytopenia, six cases of inflammatory disease, three samples with myelotoxicity and two samples analysed for staging of neoplasia. Automated white blood cell (WBC) counts correlated significantly with smear cellularity, particle cellularity and particle number. There was a significant difference in WBC counts of samples with insufficient versus sufficient particles. Significant correlations between Advia percent neutrophils and microscopical determination of marrow segmented neutrophils/neutrophilic granulocyte reserve, Advia percent lymphocytes and microscopical determination of lymphocytes/rubricytes, Advia percent large unstained cells and microscopical determination of myeloblasts/promyelocytes and between Advia percent eosinophils and manual determination of eosinophils were identified. This suggested that Advia WBC counts may be used to approximate BM sample quality and that Advia differential counts may predict marrow granulocyte reserve and lymphocyte/rubricyte stores. Distinct and consistent alterations in cytogram patterns were observed in cases of acute leukaemia, but were less obvious in chronic leukaemia. Complete automated BM analysis was performed in approximately 2 min, while staining and coverslipping of BM slides required approximately 30 min. Hence, although automated analysis should not supplant microscopical evaluation of BM, it can provide useful ancillary information in a short time and flag potentially inadequate or abnormal samples. PMID:24726417

Tan, E; Abrams-Ogg, A C G; Defarges, A; Bienzle, D

2014-07-01

308

Variation of 3 ?-to-2 ? ratio from 18F in haematological components measured using the GAMMASPHERE  

NASA Astrophysics Data System (ADS)

The potential of incorporating three-quanta annihilation into positron emission tomography (PET) to detect local tissue chemistry was investigated using the GAMMASPHERE facility, which features a spherical array of 110 Compton-suppressed high-purity germanium (HPGe) detectors. Fluoro-2-deoxyglucose (FDG) containing F18 was introduced into 11 samples of haemolysed blood, serum, cell concentrate and whole blood, some of which had been either oxygenated or deoxygenated. The relative three-quanta yield was estimated from the reduced counts in the full-energy photopeak at 511 keV. Compton-suppression produced larger effects in the calculations than time-gating, suggesting its importance for this method of estimation. The relative three-quanta yields were found to vary as much as 11% between the samples. This level of sensitivity to different biological samples commends three-quanta annihilation for molecular imaging.

Chin, M. P. W.; Seweryniak, D.; Alkhorayef, M.; Spyrou, N. M.

2009-06-01

309

Impact of copper on haematological profile of freshwater fish, Channa punctatus.  

PubMed

The present study was to evaluate heavy metal toxicity stress symptoms in fish blood during long-term exposure of sublethal concentration of copper sulphate (CuSO4). The effects of copper on various heamatological parameters were evaluated exposing Indian fresh water fish, Channa punctatus to a sub-lethal concentration of copper sulphate (0.36 mg l(-1)) for different periods. Exposure of fish to copper showed a significant decrease in the haemoglobin (Hb) content from 10.73 to 6.60%, red blood cells (RBC) from 2.86 to 1.84 x 10(6)/mm3 and packed cell volume (PCV) from 31.00 to 23.33% at the end of 45th day as compared to control. Whereas the white blood cells (WBC) increased from 60.00 to 92.48 x10(3)/mm3, clotting time (CT) from 27.66 to 43.00 second, erythrocyte sedimentation rate from 5.0 to 13.66 mm/hr and mean corpuscular volume from 108.11 to 126.85 cmu, significantly, with increase in exposure periods. Although mean corpuscular haemoglobin concentration (MCHC) and mean corpuscular haemoglobin (MCH) values showed significant increase during 15 and 30 days exposures, both the values were found significantly increased at the end of the experimental period. The differential leucocytes count (DLC) showed a significant increase in the populations of lymphocytes and eosinophils, whereas neutrophils, monocytes and basophiles were found decreased after exposure to copper. PMID:18831385

Singh, Dharam; Nath, Kamlesh; Trivedi, S P; Sharma, Y K

2008-03-01

310

Current methods to reduce the adverse haematological consequences of cardiopulmonary bypass.  

PubMed

Bleeding remains a significant cause of morbidity after cardiac surgery and results in a major demand on blood transfusion capacity. The aetiology is multifactorial, but platelet dysfunction is the most important cause. Because of the potential risks of heterologous blood transfusion, a number of techniques have been developed to reduce its need. These can broadly be divided into three categories. First, modification of bypass methods and the use of agents such as aprotinin can reduce blood loss. Second, more use can be made of the patient's own blood through predonation of autologous blood or blood conservation techniques. Finally, transfusion policy can be modified so that a lower postoperative haemoglobin level is accepted. However, a method with which to preoperatively predict those patients who will bleed significantly remains elusive. PMID:8759566

John, L C; Deverall, P B

1996-06-01

311

Haematological and serum biochemical profiles of buffalo heifers as influenced by levamisole  

Microsoft Academic Search

Serum was separated from blood samples of six healthy buffalo heifers (1–1.5 years) on day 0 (control) and after 1, 7 and 14 days of levamisole (15 mg\\/kg body weight) administered orally. Significant differences were observed among monocytes, ESR (1 h) and of percentage PCV (Packed Cell Volume) after 1 and 7 days of levamisole administration. Mean corpuscular volume (MCV)

Zia-ur-Rahman Æ; M. A. Sandhu; T. Ahmad

2003-01-01

312

[Cryptosporidiosis in newborn calves in Ankara region: clinical, haematological findings and treatment with Lasalocid-NA].  

PubMed

In the first part of the present study, a total of 109 faeces samples collected from calves suffering from diarrhoea were examined for Cryptosporidium parvum oocytes and 39 (35.8%) of them were found to be positive. On the basis of oocyte counts, 14 (36%) samples were assessed as mildly infected and 25 (64%) samples as heavily infected. The occurrence of the disease was more common in winter (56.4%) than during other seasons (autuma 0%, summer 15.4% spring 28.2%. In the present study, the ionophore polyetherantibiotic Lasalocid-Na, that is licensed as a feed additive (Bovatec, 15% Lasalocid-Na, Roche AG) in Turkey, was administered to 11 calves naturally infected with Cryptosporidium and its therapeutic effect was evaluated. Lasalocid-Na (8 mg/ kg BW) was given once daily for 3 days added to the milk. The clinical parameters of infected calves were evaluated before and 3 days after the treatment in 24 hour intervals. The oocyst counts of faeces of calves with cryptosporidiosis were between 15 x 10(6) and 96 x 10(6)/mL before treatment. No oocystes were found in faecal samples of 3 calves (27.3%) after 48 hours and 4 (40%) calves after 72 hours of treatment, respectively. The number of oocytes in the faeces of the remaining calves varied between 90 and 1.2 x 10(6)/mL during the respective period. The number of oocystes before treatment was significantly higher than the number of oocytes after treatment. One of the calves died 56 h after the first treatment despite the treatment. The pH of venous blood was decreased prior to treatment as expected. The lowest pH was 6.83, the lowest bicarbonate concentration was 3.80 mmol/l and the lowest base excess was -31.2 mmol/l. After the treatment, pH, pCO2, HCO3- and BE values of the venous blood increased significantly and reached physiological values before discharge. The differences between the values assessed before the treatment and at the 2nd, 3rd, and 4th sampling time were statistically significant. Number of leucocyte and haemoglobin concentration before the treatment were significantly higher than the values after treatment (p < 0.01). These values returned back to physiological ranges 72 hours after first treatment. Lasalocid-Na was rather well tolerated. Side effects such as the decline of the suckling reflex and intoxication symptoms in respect to present administration form and dosage were found only in one calf (9%). Treatment procedure in the present study with Lasalocid-Na was found to be suitable in combination with an adequate infusion therapy for the treatment of calves on farms with problems related to cryptosporidiosis. Although the use of Lasolacid-Na for the treatment of Cryptosporidium infection in the EU is banned, it might be used as an alternative drug outside of the EU since it has a successful effect for preventing reinfections. PMID:16028484

Sahal, M; Karaer, Z; Yasa Duru, S; Cizmeci, S; Tanyel, B

2005-06-01

313

Haematological Parameters of Fayoumi, Assil and Local Chickens Reared in Sylhet Region in Bangladesh  

Microsoft Academic Search

2 Abstract: An experiment was conducted to study the hematological parameters in Fayoumi, Assil and Local Chickens of different ages reared in Sylhet region. 250 chickens of three breeds (100 Fayoumi, 50 Assil and 100 Local) were tested at different ages (1, 3, 6, 9 and 12 months) to observe the hematological parameters: i) Total erythrocyte Count (TEC) ii) Packed

2004-01-01

314

Guidelines on product liability for the hospital blood bank. The British Committee for Standards in Haematology.  

PubMed

This report aims to clarify the position of the hospital blood bank in the light of product liability legislation contained in the Consumer Protection Act of 1987. Blood has been defined a 'product' under this Act. The potential for the blood bank to be seen in the role of 'supplier', 'keeper' or even 'producer' in the chain of product supply to the patient is explained and advice given on the resulting implications for blood bank practice. It will be necessary to define, adopt and implement standard operating procedures (SOP) for all blood bank activities. Guidance is given on the format, preparation and content of SOPs and specimen examples offered. The fundamental importance of quality assurance is emphasized. PMID:2272161

1990-01-01

315

Chronic toxicity of nonylphenol and ethinylestradiol: haematological and histopathological effects in juvenile Common carp ( Cyprinus carpio)  

Microsoft Academic Search

In recent ecotoxicological research, there is an increasing concern about alkylphenolic industrial chemicals, such as nonylphenol (NP), because of their estrogenic properties. Data on the general fish toxicity of these wide spread aquatic pollutants are scarce. In order to evaluate sublethal toxic effects of environmentally relevant concentrations of NP, juvenile Common carp (Cyprinus carpio) were exposed to NP concentrations ranging

J. Schwaiger; O. H Spieser; C. Bauer; H. Ferling; U. Mallow; W. Kalbfus; R. D. Negele

2000-01-01

316

Alfred Francois Donné (1801-78): a pioneer of microscopy, microbiology and haematology.  

PubMed

Alfred François Donné is widely known in the scientific community as the discoverer of Trichomonas vaginalis, since he was the first to illustrate the parasite that later was recognized to cause vaginal infections. However, his other, less-known findings are equally important: he was also the inventor of the photoelectric microscope, with the assistance of his student Léon Foucault, as well as the first to apply photography to microscopic preparations (Daguerreotype). His research in microscopy extended to almost all human fluids that could be investigated and culminated in his famous Atlas, which was illustrated with numerous photographs. Donné was also the first to describe the microscopic appearances of leukaemia based on blood preparations acquired from patients. Finally, his work in the hygiene of child upbringing and nutrition is very significant. PMID:19401511

Diamantis, Aristidis; Magiorkinis, Emmanouil; Androutsos, George

2009-05-01

317

Some Haematological and Biochemical Investigations on Duck Virus Hepatitis following Administration of Glycyrrhizin.  

PubMed

The present study aimed to investigate the protective effect of glycyrrhizin (locally isolated and purified from licorice root) against duck hepatitis virus through the assessment of some hematological and biochemical parameters. One hundred and sixty white Pekin ducklings-one day old-were randomly divided into four equal groups. Group (1) was kept as normal control. Group (2) was inoculated I/P with 10?mg glycyrrhizin/kg BW, three times per week for four weeks. Group (3) was inoculated I/M with 0.5?ml of live attenuated DHV vaccine. Group (4) was inoculated with both glycyrrhizin (10?mg/kg BW I/P, three times per week for four weeks) and live attenuated DHV vaccine (0.5?ml, I/M). Then, all groups of treatment were challenged using virulent DHV except for 20 ducklings from the normal control group which were continued to be kept as negative control. The results revealed that duck hepatitis virus (DHV) caused macrocytic hypochromic anemia, leukopenia, hypoproteinemia, hypoalbuminemia, hyperglycemia, hypercholesterolemia, and marked elevation of liver enzymes and renal parameters. In conclusion, glycyrrhizin injected alone or in combination with DHV vaccine protected or ameliorated the deteriorating effects induced by DHV vaccine and/or duck hepatitis virus infection by improvement of erythrogram and leukogram, as well as liver and kidney functions. PMID:23984091

Okda, Faten A; Yassein, Safaa; Ahmed, Alaa R; Soufy, Hamdy; Nasr, Soad M

2013-01-01

318

Guidelines for the investigation of invasive fungal infections in haematological malignancy and solid organ transplantation  

Microsoft Academic Search

Invasive fungal infections are increasing in incidence and now affect as many as 50% of neutropenic\\/bone marrow transplant\\u000a patients and 5 to 20% of solid organ transplant recipients. Unfortunately, many of the diagnostic tests available have a low\\u000a sensitivity. The guidelines presented here have been produced by a working party of the British Society for Medical Mycology\\u000a in an attempt

D. W. Denning; E. G. V. Evans; C. C. Kibbler; M. D. Richardson; M. M. Roberts; T. R. Rogers; D. W. Warnock; R. E. Warren

1997-01-01

319

The effects of air quality on haematological and clinical parameters in children with sickle cell anaemia  

Microsoft Academic Search

Sickle cell anaemia (SCA; HbSS) is characterised by its clinical variability, which is only partly explained by known genetic\\u000a factors. Environmental factors are known to contribute to acute problems but their importance in chronic complications has\\u000a not been analysed. We have studied 93 children with SCA in a single institution, who underwent transcranial Doppler scanning\\u000a and steady-state blood tests in

Hrishi Mittal; Lara Roberts; Gary W. Fuller; Sandra O’Driscoll; Moira C. Dick; Sue E. Height; Swee Lay Thein; David C. Rees

2009-01-01

320

Guidelines for the management of thrombophilia. Department of Haematology, The Royal London Hospital, Whitechapel, London, UK.  

PubMed Central

Although there are numerous risk factors for venous thromboembolic disease, the term 'thrombophilia' refers only to those familial or acquired disorders of the haemostatic system that result in an increased risk of thrombosis. The inherited thrombophilias include antithrombin III deficiency, resistance to activated protein C (factor V Leiden), protein C and protein S deficiencies as well as some rare forms of dysfibrinogenaemia. It is possible that other inherited conditions might also predispose to thrombosis. In contrast, when using the above definition, the antiphospholipid syndrome is the only genuine acquired thrombophilic state. Patients who have thromboembolic disease at a young age with no provoking event or who have a positive family history or whose thrombosis involves an unusual site should be investigated for thrombophilia. The management of a patient identified as having a laboratory abnormality associated with thrombophilia will depend on a variety of factors such as the patient's individual and family thrombotic history, the site of the thrombosis and the presence of other prothrombotic risk factors. The use of prophylactic anticoagulation during pregnancy and the puerperium requires particularly careful consideration in thrombophilic women. As more becomes known about the thrombophilias it will become possible to formulate more exact guidelines as to the management of these conditions.

Cavenagh, J. D.; Colvin, B. T.

1996-01-01

321

[Surveillance study of vancomycin resistant enterococci in pediatric haematology and oncology patients].  

PubMed

In this study, the prevalence and risk factors of fecal carriage of vancomycin resistant enterococci (VRE) among patients in Ondokuz Mayis University Pediatric Hematology and Oncology Clinics have been investigated. During two months, rectal swab samples were collected weekly from all of the patients hospitalized in Pediatric Hematology and Oncology Clinics, for the surveillance cultures. During the study a total of 85 rectal swabs were obtained from 34 patients who had been hospitalized in the inpatient clinic with 20 bed capacity. The number of samples obtained from the patients varied between 1-6 cultures. All of the patients (100%) had peripheral venous catheters and 27(79%) of patients had used antibiotics. All of the samples were inoculated onto 8 microg/ mL gentamicin containing blood agar media, and enterococci were identified by Gram staining, catalase test and at species level by VITEK 2 (bioMérieux, France) automated system. Vancomycin resistance was screened by using 6 microg/mL vancomycin containing brain-heart infusion agar according to CLSI guidelines. The vancomycin MIC values of the strains grown in this medium were determined by microdilution test proposed by CLSI. As a result, a total of 50 samples (59%) belonging to 24 patients yielded enterococci, and the species distribution was as follows; E. faecium (in 16 cases), E. faecalis (in 8 cases), E. casseliflavus (in 6 cases), E. avium (in 3 cases) and E. durans (in 1 case). In our study no vancomycin resistance nor VRE colonization was detected in the patients. PMID:17001854

Ta?delen Fi?gin, Nuriye; Darka, Ozge; Fi?gin, Tunç; Hepsert, Serkan; Coban, Ahmet Yilmaz; Elli, Murat

2006-07-01

322

[Inflammatory response and haematological disorders in cardiac surgery: toward a more physiological cardiopulmonary bypass].  

PubMed

The systemic inflammatory response in cardiac surgery is closely related to the haemostasis disturbances. It is responsible of a significant morbidity and mortality that was previously suspected to be caused by cardiopulmonary bypass alone. However, it is time now to clearly identify the factors that are material-dependent from that material-independent. From this point of view, off-pump surgery allowed for better comprehension of the multiple sources of the inflammatory response. Numerous pathways are activated, involving complement, platelets, neutrophiles and monocytes. The tissue pathway of the coagulation system, through tissue factor, is of major importance and has to be surgically considered in order to reduce the whole body inflammatory response postoperatively. The quality of the extracorporeal perfusion through its consequences on organ perfusion, particularly in the splanchnic area, also participates to this pathophysiological process. Beyond the progress of technology provided by the industry, particularly the minimally extracorporeal circulation derived from off-pump surgery evolution, the surgical approach is of major importance in the control of the systemic inflammatory response and must not be ignored yet. PMID:16488106

Baufreton, C; Corbeau, J-J; Pinaud, F

2006-05-01

323

Haematological characteristics predicting susceptibility for ascites. 2. High haematocrit values in juvenile chickens  

Microsoft Academic Search

1. Male broilers of two different genetic stocks, a pure broiler sire line (A) and commercially available Ross broilers (B), were used to study the effect of differences in haematocrit values in 12-d-old chickens on the development of ascitic symptoms. Body weight gain (BWG), feed intake (FI) and feed conversion ratio (FRC) were measured from 2 to 5 weeks of

C. W. Scheele; J. D. Van Der Klis; C. Kwakernaak; N. Buys; E. Decuypere

2003-01-01

324

Clinical, haematological and biochemical studies of babesiosis in native goats in Mosul  

Microsoft Academic Search

The study included examination of 175 native goats, 27 were infected with Babesia ovis , B. motasi , B. foliata and B. taylori , (recorded in Mosul for the first time) and 25 were clinically normal and served as control. Results indicated that the percentage of the infection with Babesiosis was 15.42% and the percentage of parasitemia ranged between 3.5-10.4%

E. G. Sulaiman; S. H. Arslan; Q. T. Al-Obaidi; E. Daham

325

Heterogeneity and Variation of Clinical and Haematological Expression of Haemoglobin S in Saudi Arabs  

Microsoft Academic Search

Sickle cell haemoglobin (Hb S) occurs at a high frequency in the Eastern (EP), South-Western (SWP) and North-Western (NWP) Provinces of Saudi Arabia and the presentation of the Hb S is believed to exhibit clinical diversity in the different regions. Three areas of Saudi Arabia were screened to determine the frequency of Hb S and ?- and ?-thalassaemias and glucose-6-phosphate

Mohsen A. F. El-Hazmi

1992-01-01

326

Haematological parameters of Lake Baikal oilfish (golomyanka) ( Comephorus dybowskii and Comephorus baicalensis )  

Microsoft Academic Search

Two endemic Baikal species of oilfish, Comephorus dybowskii (“small golomyanka”) and Comephorou baicalensis (“big golomyanka”) live in the pelagic zone down to the bottom (1600 m). Both have numerous adaptations to the pelagic habitat.\\u000a In this regard, the paper gives results of studies of blood under various natural conditions. The cell composition of peripheral\\u000a blood does not differ between the

V. M. Yakhnenko; M. S. Yakhnenko

2006-01-01

327

Estimation of the haematological toxicity of minor groove alkylators using tests on human cord blood cells  

Microsoft Academic Search

We evaluated the myelotoxicity and the anti-tumor potential of tallimustine, three of its analogues and carzelesin, with melphalan as reference substance. Tallimustine was tested by clonogenic assays on both human bone marrow (BM) and cord blood (hCB) cells, the other compounds on hCB only. The degree of inhibition of the haemopoietic progenitors GM-CFC, CFC-E and BFU-E was evaluated after exposure

M Ghielmini; G Bosshard; L Capolongo; MC Geroni; E Pesenti; V Torri; M D'Incalci; F Cavalli; C Sessa

1997-01-01

328

Association of heterocellular HPFH, beta(+)-thalassaemia, and delta beta(0)-thalassaemia: haematological and molecular aspects  

Microsoft Academic Search

An Italian family in which heterocellular hereditary persistence of fetal haemoglobin (HPFH) interacts with both beta(+)- and delta beta-thalassaemia is described. The index case was an 8 year old girl who was presumed to inherit both heterocellular HPFH and beta (+)-thalassaemia from her mother and delta beta-thalassaemia from her father. She was healthy and never needed blood transfusions. The possible

L Cianetti; A Care; N M Sposi; A Giampaolo; M Calandrini; M Petrini; A Massa; M Marinucci; F Mavilio; M Ceccanti

1984-01-01

329

Risk factors for early mortality in haematological malignancy patients with pulmonary mucormycosis.  

PubMed

Pulmonary mucormycosis (PM) is a life-threatening opportunistic mycosis with a variable clinical evolution and few prognostic markers for outcome assessment. Several clinical risk factors for poor outcome present at the diagnosis of PM were analyzed in 75 consecutive hematology patients from 2000-2012. Significant variables (P < 0.1) were entered into a multivariate Cox-proportional hazard regression model adjusting for baseline APACHE II to identify independent risk factors for mortality within 28 days. Twenty-eight of 75 patients died within 4-week follow up. A lymphocyte count < 100/mm³ at the time of diagnosis (adjusted hazard ratio 4.0, 1.7-9.4, P = 0.01) and high level of lactate dehydrogenase (AHR 3.7, 1.3-10.2, P = 0.015) were independent predictors along with APACHE II score for 28-day mortality. A weighted risk score based on these 3 baseline variables accurately identified non-surviving patients at 28 days (area under the receiver-operator curve of 0.87, 0.77-0.93, P < 0.001). A risk score > 22 was associated with 8-fold high rates of mortality (P < 0.0001) within 28 days of diagnosis and median survival of 7 days versus ?28 days in patients with risk scores ?22. We found that APACHE II score, severe lymphocytopenia and high LDH levels at the time of PM diagnosis were independent markers for rapid disease progression and death. PMID:23905713

Lewis, Russell E; Georgiadou, Sarah P; Sampsonas, Fotis; Chamilos, George; Kontoyiannis, Dimitrios P

2014-01-01

330

Does habitat fragmentation cause stress in the agile antechinus? A haematological approach  

Microsoft Academic Search

Although the vertebrate stress response is essential for survival, frequent or prolonged stress responses can result in chronic\\u000a physiological stress, which is associated with a suite of conditions that can impair survivorship and reproductive output.\\u000a Anthropogenic habitat fragmentation and degradation are potential stressors of free-living vertebrates, and in theory could\\u000a result in chronic stress. To address this issue, we compared

Christopher P. Johnstone; Alan Lill; Richard D. Reina

331

Notch signalling in T cell lymphoblastic leukaemia/lymphoma and other haematological malignancies  

PubMed Central

Notch receptors participate in a highly conserved signalling pathway that regulates normal development and tissue homeostasis in a context- and dose-dependent manner. Deregulated Notch signalling has been implicated in many diseases, but the clearest example of a pathogenic role is found in T cell lymphoblastic leukaemia/lymphoma (T-LL), in which the majority of human and murine tumours have acquired mutations that lead to aberrant increases in Notch1 signalling. Remarkably, it appears that the selective pressure for Notch mutations is virtually unique among cancers to T-LL, presumably reflecting a special context-dependent role for Notch in normal T cell progenitors. Nevertheless, there are some recent reports suggesting that Notch signalling has subtle yet important roles in other forms of hematologic malignancy as well. Here, we review the role of Notch signalling in various blood cancers, focusing on T-LL with an eye toward targeted therapeutics.

Aster, Jon C.; Blacklow, Stephen C.; Pear, Warren S.

2010-01-01

332

Periodontal infection in adult-onset Still's disease patient: clinical and haematological considerations  

PubMed Central

In this case report, the authors described the first case of a patient with adult-onset Still’s disease (AOSD) who presents advanced periodontal infection. AOSD is a rare systemic inflammatory disorder of unknown aetiology, characterised by spiking fever, usually exceeding 39°C, an evanescent salmon pink rash, arthritis and multiorgan involvement. Periodontal infection is a pathogen-induced oral inflammatory disease affecting the supporting tissues of teeth and is currently considered as a risk factor for cardiovascular disease. Several cytokines capable of inducing systemic effects are produced during the course of this infection and the values of serum markers of inflammation, such as C reactive protein (CRP), may significantly decrease after periodontal treatment. Although AOSD can produce elevations in CRP, similar increase may be produced by periodontal infection, suggesting the need for medical and dental diagnosis when evaluating the sources of acute-phase responses in systemic autoimmune disease patients.

Pessoa, Larissa; Galvao, Virgilio; Ferreira, Clarissa; Neto, Leopoldo Santos

2011-01-01

333

Evaluation of the CELL-DYN ® 3500 haematology instrument for the analysis of the mouse and rat blood  

Microsoft Academic Search

The objective of this study was to evaluate the performance of the CELL-DYN® 3500 for rat and mouse blood analysis in a routine environment. The WBC (white blood cells), RBC (red blood cells), PLT (platelets)\\u000a counts and the WBC differential were determined. In addition, the following aspects were studied: within-run precision, day-to-day\\u000a precision, biasfree paired difference precision; extended ranges of

J. Kieffer; G. Winkler; L. Van Hove; A. Walsh; P. Thomann; S. Wyss; E. Eggenberger; H. Lutz

1999-01-01

334

Physiological, haematological and histopathological responses in common carp (Cyprinus carpio L.) fingerlings fed with differently detoxified Jatropha curcas kernel meal.  

PubMed

Protein rich Jatropha curcas kernel meal is toxic. It was detoxified using heat treatment and solvent extraction. Two duration of detoxification process were investigated: shorter (30 min) and longer (60 min) and the detoxified meals so obtained were designated as J(a) and J(b) respectively. Common carp fingerlings (252 fish; 3.2+/-0.07 g) were fed with the following diets: Control containing fishmeal (FM); S(50,) J(a50) and J(b50): 50% of FM protein replaced by soybean meal (SBM), detoxified Jatropha kernel meal (DJ(a)KM and DJ(b)KM); S(75), J(a75) and J(b75): 75% of FM protein replaced by SBM, DJ(a)KM and DJ(b)KM. White blood cells count, mean cell volume and mean cell haemoglobin concentration, calcium and sodium ions and total bilirubin in blood did not differ significantly among the groups. Higher (P>0.05) RBC count was observed in plant protein fed groups compared to control group. Highest alkaline phosphatase and alanine transaminase activities in blood were observed in J(a75), which were not different (P>0.05) from those in J(a50) group, but were higher than in the other groups. No adverse histopathological changes in liver and muscle of any group were observed, but intestinal mucosa of J(a75) groups showed severe pathological lesions. The results demonstrate that Jb was completely detoxified. Since the performance of J(b50) group was similar to control group and better than the other groups, optimum inclusion level of J(b) is 50% replacement of FM protein. PMID:20457206

Kumar, Vikas; Makkar, Harinder P S; Amselgruber, Werner; Becker, Klaus

2010-01-01

335

Serum Copper and Haematological Values of Sheep of Different Physiological Stages in the Dry and Wet Seasons of Central Trinidad  

PubMed Central

A study was conducted to determine serum copper (Cu) concentrations and hematological values of 174 sheep from four medium sized farms, pertaining to physiological stage, in the late dry and late wet seasons of Central Trinidad. Serum Cu was significantly lower in the dry than in the wet season (P < 0.001) with a corresponding high percentage of samples with low Cu levels in the former. 31% of dry nonpregnant sheep exhibited a nonregenerative anaemia. Also, hemoglobin and packed cell volume values varied (P < 0.001) including lymphocyte (P < 0.01) counts, among growing animals compared with other physiological stages. Significant variations also occurred among neutrophil (P < 0.05) and eosinophil (P < 0.05) values in sheep. Highest haemoglobin and packed cell volume, white blood cell counts, and lymphocyte values in growing sheep compared with other stages were probably age related.

Mohammed, A.; Campbell, M.; Youssef, F. G.

2014-01-01

336

Pharmacokinetics and effects on bowel and throat microflora of oral levofloxacin as antibacterial prophylaxis in neutropenic patients with haematological malignancies  

Microsoft Academic Search

Gram-positive breakthrough infections pose a major drawback to the use of quinolones for antibacterial prophylaxis in neutropenic patients. Levofloxacin offers the advantage of an augmented Gram-positive spectrum and may potentially overcome this problem. In an open-label, clinical pilot study, we investigated the effects on throat and bowel microflora and pharmacokinetics of a once-daily oral dose of 500 mg levofloxacin, during

G. J. Timmers; Y. Dijstelbloem; A. M. Simoons-Smit; A J van Winkelhoff; D J Touw; C. M. J. E. Vandenbroucke-Grauls; P C Huijgens

2004-01-01

337

Evaluation of the nutritional and inflammatory status in cancer patients for the risk assessment of severe haematological toxicity following chemotherapy  

Microsoft Academic Search

Results: Patients experiencing SHT had a higher PS (P <0.001), inflammatory serum protein levels (P <0.001) and NIS ratio (P <0.0001), but lower haemoglobin (P <0.05) and serum-albumin levels (P <0.0001). Using a cut-off of 0 or 1 for PS and 1 for NIS, sensitivity was 98%, 43% and 89%; specificity was 38%, 90% and 66%, respectively. In 37 patients

J. Alexandre; M. Gross-Goupil; B. Falissard; M.-L. Nguyen; J.-M. Gornet; J.-L. Misset; F. Goldwasser

2003-01-01

338

Concurrent infections with vector-borne pathogens associated with fatal anaemia in cattle: haematology and blood chemistry  

Microsoft Academic Search

An outbreak of a fatal haemolytic anaemia in a dairy herd of cattle in Switzerland was shown to be associated with infections\\u000a with five vector-borne pathogens, namely Anaplasma marginale, A. phagocytophilum, Babesia bigemina, a Theileria spp belonging to the buffeli\\/sergenti\\/orientalis complex and haemotrophic Mycoplasma spp. The latter three had not been documented before this outbreak in Switzerland. To characterise the

Barbara Riond; Marina L. Meli; Ueli Braun; Peter Deplazes; Kaspar Joerger; Rudolf Thoma; Hans Lutz; Regina Hofmann-Lehmann

2008-01-01

339

Zinc toxicosis of holstein veal calves and its relationship to haematological change and an associated thrombotic state  

Microsoft Academic Search

Zinc toxicosis has been reported with increasing frequency in livestock species. This report describes changes in haemograms, prothrombin time, activated partial thromboplastin time (APTT), fibrinogen concentration and antithrombin III activities in zinc-toxic calves (706 µg zinc\\/g diet), compared to control calves (100 µ zinc\\/g diet). When comparing differential leucocyte counts in calves before and during exposure to high zinc, there

T. W. Graham; B. F. Feldman; T. B. Farver; F. Labavitch; S. L. O'Nei; M. C. Thurmond; C. L. Keen; C. A. Holmberg

1991-01-01

340

Cardiorespiratory, hormonal and haematological responses to submaximal cycling performed 2 days after eccentric or concentric exercise bouts  

Microsoft Academic Search

Eccentric muscle actions are known to induce delayed?onset muscle soreness (DOMS) and muscle weakness (reduced static strength and dynamic peak power output) that may persist for several days. The aim of the present study was to determine whether DOMS?inducing exercise affects physiological responses to subsequent submaximal dynamic exercise. Physiological and metabolic responses to a standardized exercise task were measured 2

Michael Gleeson; Andrew K. Blannin; Bin Zhu; Stephen Brooks; Robert Cave

1995-01-01

341

Cardiorespiratory, hormonal and haematological responses to submaximal cycling performed 2 days after eccentric or concentric exercise bouts.  

PubMed

Eccentric muscle actions are known to induce delayed-onset muscle soreness (DOMS) and muscle weakness (reduced static strength and dynamic peak power output) that may persist for several days. The aim of the present study was to determine whether DOMS-inducing exercise affects physiological responses to subsequent submaximal dynamic exercise. Physiological and metabolic responses to a standardized exercise task were measured 2 days after the performance of an eccentric or concentric exercise bout. Six healthy, untrained male subjects aged 30 +/- 7 years (mean +/- S.D.) performed repeated eccentric contractions during 30 min of bench stepping (47-cm step, 15 steps min-1). On another occasion, they performed concentric contractions by walking uphill (8% incline) for 30 min at 5 km h-1, which elicited a similar heart rate response to bench stepping. Two days after the eccentric or concentric exercise, the subjects cycled for 15 min on an electrically braked cycle ergometer at a work rate (172 +/- 37 W) equivalent to 80% VO2 max. The order of the preceding treatments was randomized and the treatments were carried out 2 weeks apart. Two days after the eccentric exercise, all subjects reported leg muscle soreness and exhibited elevated levels of serum creatine kinase activity (P < 0.01) and plasma cortisol concentration (P < 0.05). After uphill walking, the subjects were not sore and serum creatine kinase activity was unchanged. Minute volume, breathing frequency, respiratory exchange ratio, heart rate, rating of perceived exertion, venous blood lactate concentration and plasma cortisol concentration were all higher (P < 0.05) during cycling after eccentric exercise compared with after uphill walking. Increases in plasma catecholamine concentrations and numbers of circulating leucocytes after cycling at 80% VO2 max for 15 min were similar under both experimental conditions, but the delayed leucocytosis (at 150 min post-exercise) was significantly greater (P < 0.01) for the post-eccentric exercise condition. We conclude that dynamic submaximal exercise performed 2 days following exercise with a large eccentric component produces physiological responses that are indicative of a higher relative exercise stress. It is likely that such effects will significantly limit the level and duration of exercise that can be achieved in subsequent training bouts over several days. PMID:8850573

Gleeson, M; Blannin, A K; Zhu, B; Brooks, S; Cave, R

1995-12-01

342

The influence of enterosorption on some haematological and biochemical indices of the normal rats after single injection of melphalan.  

PubMed

Aim: One of the most prominent side effects of intensive cancer chemotherapy is bone marrow suppression which is an independent negative prognostic factor for the time to tumor progression. The aim of the study was to evaluate the myeloprotective possibilities of carbon enterosorbents in the case of usage of alkilating drug melphalan (L-PAM). Materials and Methods: L-PAM was injected intravenously to healthy inbred rats to cause the myelosuppression. 3 days before and 7 days after this, suspension of two types of carbon granulated enterosorbents were administered per os one time per day. On 8(th) day after L-PAM injection, the rats were weighted and blood and liver tissue were taken under Ketamine general anesthesia for biochemical examination. Peripheral blood smears were made also. Results: Melphalan at a dose of 3 mg/kg causes expressed myelotoxic reaction: leucopenia, decre-asing of erythrocytes, hemoglobin and platelets counts. Even on 8(th) day after single injection of this cytostatic we can detect expressed signs of oxidative stress like increasing of hydroperoxides, TBA-reactive substances, and decreasing of activity and level of main endogenic antioxidants - superoxide dismutase (SOD), catalase and reduced glutathione. L-PAM causes also the violation of kidney function such as increase of urea and creatinine level; and rising of endogenic intoxication with elevation of middle mass molecules level. In a dose of 3 mg/kg melphalan has no negative influence on liver function on 8(th) day of experiment. Enterosorption with carbon enterosorbents C1 (bulk density ? = 0.28 g/cm(3), granules diameter 0.15-0.25 mm, BET pore surface 1719 m(2)/g, therapeutic dosage 1400 mg/kg) and C2 (bulk density ? = 0.18 g/cm(3), granules diameter 0.15-0.25 mm, BET pore surface 2162 m(2)/g, therapeutic dosage 900 mg/kg) diminishes and mitigates negative side effects caused by single intravenous injection of melphalan. Carbon enterosorbent C2 have rather more expressed positive effect than C1 for practically all indices. The most important curative effect due to C2 administration is prominent myeloprotection of bone marrow of experimental animals. Conclusion: Carbon enterosorbent C2 is promising and perspective sorbent for prophylaxis and treatment of side effects of cytostatic chemotherapy including myelotoxicity, mucositis, kidney injuries, gonadotoxicity, etc. PMID:24980763

Shevchuk, O O; Posokhova, K A; Sidorenko, A S; Bardakhivskaya, K I; Maslenny, V M; Yushko, L A; Chekhun, V F; Nikolaev, V G

2014-06-01

343

Comparative Haematological Screening of Urban and Rural Pregnant Women Attending Antenatal Clinics in Lagos and Its Environs.  

ERIC Educational Resources Information Center

Compared blood screening data for 200 urban and rural pregnant women in Nigeria. Found that rural subjects had a greater incidence of moderate anemia than did urban subjects, and corpuscular hemoglobin concentrations fell with increased gestational age. No relationship was found between hemoglobin counts and nutrition habits. (HTH)

Abidoye, R. O.; Olukoya, A. A.

1993-01-01

344

Comparison of haematological values in restricted?and ad libitum?fed domestic fowls: White blood cells and thrombocytes  

Microsoft Academic Search

1. White blood cells and thrombocyte values were examined in normal domestic fowls of layer and broiler strains fed restricted or ad libitum diets.2. Basophils were the only white blood cells to increase significantly in number after the period of food restriction. It is suggested that the increase may have been associated with stress.3. There were more basophils in broilers

M. H. Maxwell; G. W. Robertson; S. Spence; C. C. McCorquodale

1990-01-01

345

Haematological and immunological effects of repeated dose exposure of rats to integerrimine N-oxide from Senecio brasiliensis  

Microsoft Academic Search

This study is the first in the literature to focus attention on the possible immunotoxic effect of integerrimine N-oxide content in the butanolic residue (BR) of Senecio brasiliensis, a poisonous hepatotoxic plant that contains pyrrolizidine alkaloids (PAs). PAs have been reported as a pasture and food contaminant and as herbal medicine used worldwide and are responsible for poisoning events in

Fabiana Elias; Andreia O. Latorre; Fernando Pípole; Mitsue Haraguchi; Silvana L. Górniak; Isis M. Hueza

2011-01-01

346

Acute intoxication of deltamethrin in monosex Nile tilapia, Oreochromis niloticus with special reference to the clinical, biochemical and haematological effects  

Microsoft Academic Search

Deltamethrin, a synthetic pyrethroid pesticide, potential toxic pollutant and significant direct risk to the aquatic ecosystems, was investigated in the present study for its toxic impact on adult monosex Nile tilapia on the basis of acute static bioassay test, and comparison of clinical, biochemical and haemato logical examinations with deltamethrin-free control group. The 96hLC50 value of deltamethrin for monosex tilapia

Y. S. El-Sayed; T. T. Saad; S. M. El-Bahr

2007-01-01

347

Effect of caffeine supplementation on haematological and biochemical variables in elite soccer players under physical stress conditions  

Microsoft Academic Search

Objective: To evaluate the effect of caffeine on white cell distribution and muscle injury markers in professional soccer players during exercise.Methods: 22 male athletes completed a placebo controlled double blind test protocol to simulate a soccer match, followed by a Yo-Yo intermittent recovery test.Results: Exercise caused an increase in packed cell volume that was enhanced by caffeine. Caffeine and exercise

Adriana Bassini-Cameron; Eric Sweet; Altamiro Bottino; Christina Bittar; Carlos Veiga; Luiz-Claudio Cameron; E. H M Dantas

2007-01-01

348

Relation between some haematological abnormalities, degree of immunosuppression and viral load in treatment-naïve HIV-infected patients.  

PubMed

This cross-sectional study at a teaching hospital in north-eastern Nigeria estimated the prevalence of anaemia, leukopenia and thrombocytopenia in treatment-naïve HIV-infected patients (177 males and 316 females), and the associations with virological and immunological markers. The overall prevalences of anaemia, leukopenia and thrombocytopenia were 49.5%, 5.5% and 4.5% respectively. The prevalence of anaemia was significantly higher in males than females (61.6% versus 42.7%), while the rates of leukopenia (5.1% versus 5.7%) and thrombocytopenia (5.7% versus 3.8%) were similar. Almost two-thirds of the HIV treatment-naïve studied patients, 293/493 (59.4%), had cytopenia and would require antiretroviral drugs. AIDS was diagnosed by clinical or immunological criteria in 70% of patients. The degree of cytopenia was directly related to the degree of immunosuppression and clinical AIDS status. No relationship was observed between cytopenia and viral load. PMID:23882962

Denue, B A; Gashau, W; Bello, H S; Kida, I M; Bakki, B; Ajayi, B

2013-04-01

349

Haematological complete remission by ponatinib and bortezomib in a patient with relapsed, Ph+ pre-B acute lymphoblastic leukaemia.  

PubMed

A 74-year-old man was previously diagnosed with BCR-ABL1-positive pre-B cell acute lymphoblastic leukaemia (pre-B ALL) based on bone marrow cytology, flow cytometry, cytogenetics and fluorescent in situ hybridisation findings. Following a highly complicated hospital course, the patient achieved cytogenetic remission by consolidated chemotherapy and the tyrosine kinase inhibitor dasatinib. He subsequently presented with relapsed pre-B ALL after 3 years in remission. In consideration of his challenging clinical history, he was started on concurrent ponatinib (45 mg daily) and bortezomib (1.3 mg/m(2) intravenous weekly). The major molecular response was achieved (<0.0893% BCR-ABL1 transcripts) after 3 months. Bone marrow now demonstrates a BCR-ABL1-negative, complete cytogenetic response. The patient continues to do well with mild thrombocytopenia and improved anaemia on bortezomib and 30 mg daily ponatinib. Our experience with a single patient suggests the feasibility of combined targeted therapy with ponatinib and bortezomib. This novel treatment approach achieved clinical remission with a manageable toxicity profile. PMID:24729118

Robinson, Sara; Levy, Yair; Maisel, Christopher; Tong, Alex W

2014-01-01

350

Distribution of filamentous fungi causing invasive fungal disease at the Haematological Unit, Hospital de Clínicas de Porto Alegre, Brazil.  

PubMed

Very limited data are available in the literature to elucidate the aetiology of invasive mould infections in Latin America. Here we report that Aspergillus species caused only half of such cases in a cohort study conducted over 21 months in a university hospital in Porto Alegre, Southern Brazil. Fusarium spp. were the second most prevalent moulds (20.7%), followed by Zygomycetes (13.8%). The importance of obtaining local epidemiological data for adequately guiding empirical antifungal therapy is reinforced. PMID:20835512

Aquino, Valério Rodrigues; Verçosa, Emanuelle Bergonsi; Falhauber, Gustavo; Lunardi, Luciano Werle; Silla, Lucia; Pasqualotto, Alessandro Comarú

2010-01-01

351

Haematological and biochemical changes in Fasciola gigantica infected Buffaloes fed on diet containing deoiled mahua (Bassia latifolia) seed cake  

Microsoft Academic Search

A study was conducted to evaluate the effect of condensed tannins and saponins from deoiled mahua seed cake (DMSC) on the development of fasciolosis. Fifteen male buffaloes were randomly divided into 3 groups of five each and were orally infected with F. gigantica metacercaria @ 800\\/animal. DMSC was included in the concentrate mixture of group 2 (M5) and 3 animals

P. Singh; A. K. Verma; Ani Bency Jacob; S. C. Gupta; U. R. Mehra

2011-01-01

352

Invasive Candida Infections in Patients With Haematological Malignancies and Hematopoietic Stem Cell Transplant Recipients: Current Epidemiology and Therapeutic Options.  

PubMed Central

In the last decades, the global epidemiological impact of invasive candidiasis (IC) in patients with hematologic malignancies (HM) and in hematopoietic stem cell transplant (HSCT) recipients has decreased and the incidence of invasive aspergillosis exceeded that of Candida infections. The use of prevention strategies, first of all antifungal prophylaxis with triazoles, contributed to the reduction of IC in these populations as demonstrated by several epidemiological studies. However, relatively little is known about the current epidemiological patterns of IC in HM and HSCT populations, because recent epidemiological data almost exclusively derive from retrospective experiences and few prospective data are available. Several prospective, controlled studies in the prophylaxis of invasive fungal diseases have been conducted in both the HM and HSCT setting. On the contrary, most of the prospective controlled trials that demonstrated the efficacy of the antifungal drugs echinocandins and voriconazole in the treatment of candidemia and invasive candidiasis mainly involved patients with underlying conditions other than HM or HSCT. For these reasons, international guidelines provided specific indications for the prophylaxis strategies in HM and HSCT patients, whereas the recommendations on therapy of documented Candida infections are based on the results observed in the general population and should be considered with caution.

Girmenia, Corrado; Finolezzi, Erica; Federico, Vincenzo; Santopietro, Michelina; Perrone, Salvatore

2011-01-01

353

HISTOLOGICAL CHANGES AND CHRONOLOGICAL TRANSITIONS OF THE BONE MARROW OF A- BOMB SURVIVORS DYING OF NON-HAEMATOLOGICAL DISORDERS  

Microsoft Academic Search

Bone marrow from the vertebrae and sternum of 35 cases of cardiovascu1ar ; disease, 33 of pulmonary tuberculosis, 18 of gastric carcinoma, 18 of carcinoma ; of other organs, and 7 of bronchopneumonia among A-bomb-exposed persons was ; compared with the marrow of non-exposed patients. Ce1lular elements were ; markedly increased, especially premature cells of the granulocytic series, and ;

S. Matsuoka; Y. Katsura

1959-01-01

354

Trigonella foenum-graecum protection against deltamethrin-induced toxic effects on haematological, biochemical, and oxidative stress parameters in rats.  

PubMed

Trigonella foenum-graecum L. is enriched with many active ingredients. TFG oil was evaluated for its protective effect against deltamethrin toxicity in rats. Rats of the control group were administered saline. The 2nd group was administered deltamethrin (DLM) orally at a concentration of 15 mg/kg body mass. The 3rd and 4th groups were administered DLM at a concentration of 15 mg/kg body mass and were fed diets containing 2.5% and 5% TFG oil, respectively. DLM intoxication reduced red blood cell and platelet counts, hemoglobin concentration, and hematocrit value while it induced leucocytosis. Furthermore, it increased serum levels of lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, ?-glutamyltransferase, triglycerides, cholesterol, uric acid, urea, and creatinine; increased hepatic, renal, and brain lipid peroxidation; decreased serum acetylcholine esterase level; and decreased hepatic, renal, and brain antioxidant markers' activities. However, TFG oil kept the studied hematological and biochemical parameters within normal ranges. In addition, it prevented lipid peroxidation and oxidative stress induced by DLM intoxication in a dose-dependent manner. Therefore, these results indicated that TFG oil inhibited the toxic effects of DLM on hematological and biochemical parameters as well as oxidative status by its free radical scavenging and potent antioxidant activities, and it appeared to be a promising protective agent against DLM-induced toxicity. PMID:25029214

Abdel-Daim, Mohamed M; Abd Eldaim, Mabrouk A; Mahmoud, Mohamed M

2014-08-01

355

Sex, morph and size-specific susceptibility to stress measured by haematological variables in captive common wall lizard Podarcis muralis  

Microsoft Academic Search

In polymorphic species of animals, colour morphs may show alternative physiological properties, and hence evolve or be maintained as an indirect response to selection exerted on these physiological attributes. In this study, we investigated if different colour morphs (white, red and yellow) of the polymorphic common wall lizard differed in their physiological responses to a long-term stress by determining variation

Paolo Galeotti; Daniele Pellitteri-Rosa; Roberto Sacchi; Augusto Gentilli; Fabio Pupin; Diego Rubolini; Mauro Fasola

2010-01-01

356

Prevalence, patterns of disease and outcome in patients with systemic lupus erythematosus who develop severe haematological problems  

Microsoft Academic Search

Objective. To evaluate the prevalence of major haemolytic disease—severe autoimmune haemolytic anaemia and severe thrombocytopenia—and to assess when these features develop. We also sought to analyse the clinical and serological outcomes of patients with haemolytic anaemia and thrombocytopenia with systemic lupus erythematosus (SLE) as compared with patients without these cytopenias. Methods. We reviewed retrospectively all the available case notes from

S. M. Sultan; S. Begum; D. A. Isenberg

2003-01-01

357

Differences in the haematological profile of healthy 70 year old men and women: normal ranges with confirmatory factor analysis  

Microsoft Academic Search

BACKGROUND: Reference ranges are available for different blood cell counts. These ranges treat each cell type independently and do not consider possible correlations between cell types. METHODS: Participants were identified from the Community Health Index as survivors of the 1947 Scottish Mental Survey, all born in 1936, who were resident in Lothian (potential n = 3,810) and invited to participate

Rowan McIlhagger; Alan J Gow; Caroline E Brett; Janie Corley; Michelle Taylor; Ian J Deary; John M Starr

2010-01-01

358

Effect of Trachelobdella lubrica (Hirudinea: Piscicolidae) on biochemical and haematological characteristics of black scorpion fish ( Scorpaena porcus , Linnaeus 1758)  

Microsoft Academic Search

Black Scorpion fish (Scorpaena porcus Linnaeus 1758) caught during monthly sampling carried out in 2003 in the Dardanelles, Turkey, were examined for the parasite. Trachelobdella lubrica. Infested fish were caught only during December 2003, and the clinical conditions of the 12 fish naturally infested with T. lubrica included fin rot, swollen foci of skin and haemorrhages of the gill and

Ekrem Sanver Celik; Seyit Aydin

2006-01-01

359

Effect of aqueous extract of Bulbine natalensis Baker stem on haematological and serum lipid profile of male Wistar rats  

Microsoft Academic Search

Bulbine natalensis stem extract (25, 50 and 100 mg\\/kg body weight for 14 days) did not significantly alter the red blood cell count, haemoglobin, packed cell volume, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and red cell distribution width in male Wistar rats. In contrast, the white blood cell count increased by the end of the experimental

M T Yakubu; A J Afolayan

2009-01-01

360

Serum copper and haematological values of sheep of different physiological stages in the dry and wet seasons of central trinidad.  

PubMed

A study was conducted to determine serum copper (Cu) concentrations and hematological values of 174 sheep from four medium sized farms, pertaining to physiological stage, in the late dry and late wet seasons of Central Trinidad. Serum Cu was significantly lower in the dry than in the wet season (P < 0.001) with a corresponding high percentage of samples with low Cu levels in the former. 31% of dry nonpregnant sheep exhibited a nonregenerative anaemia. Also, hemoglobin and packed cell volume values varied (P < 0.001) including lymphocyte (P < 0.01) counts, among growing animals compared with other physiological stages. Significant variations also occurred among neutrophil (P < 0.05) and eosinophil (P < 0.05) values in sheep. Highest haemoglobin and packed cell volume, white blood cell counts, and lymphocyte values in growing sheep compared with other stages were probably age related. PMID:24895542

Mohammed, A; Campbell, M; Youssef, F G

2014-01-01

361

Hand-held dynamometry in patients with haematological malignancies: Measurement error in the clinical assessment of knee extension strength  

Microsoft Academic Search

BACKGROUND: Hand-held dynamometry is a portable and inexpensive method to quantify muscle strength. To determine if muscle strength has changed, an examiner must know what part of the difference between a patient's pre-treatment and post-treatment measurements is attributable to real change, and what part is due to measurement error. This study aimed to determine the relative and absolute reliability of

Ruud H Knols; Geert Aufdemkampe; Eling D de Bruin; Daniel Uebelhart; Neil K Aaronson

2009-01-01

362

Influence of granite-grit on nutrient digestibility and haematological parameters of broiler chickens fed rice offal based diets.  

PubMed

A total of 270 broiler chickens were used for the study. The birds were fed common diet containing 23% Crude protein and 2864 kcal kg(-1) Metabolizable energy at the starter phase while 20% Crude protein and 2923 kcal kg(-1) Metabolizable energy was fed at the finisher phase. Starter and finisher diets contained 10 and 15% inclusion levels of rice offal respectively. Granite grit was added to the basal diet at 0.0, 2.0, 4.0, 6.0, 8.0 and 10.0 g per bird per month thus making a total of six treatments. Each treatment was replicated three times with 15 birds per replicate in a completely randomized design. Packed cell volume and haemoglobin level were not significantly (p>0.05) affected by dietary grit levels while total protein increased across the graded levels of granite grit. Crude protein, crude fibre and nitrogen free extract significantly (p<0.05) improved with increasing grit levels. These parameters improved up to the highest level of grit addition (10.0 g) granite-grit. It was concluded that 10.0 g granite grit per bird per month is beneficial to broiler chickens as it allows for efficient nutrient utilization. Further study to determine the optimum level of granite grit in broiler diet is encouraged since result obtained showed the optimum level was not attained. PMID:24502172

Idachaba, C U; Abeke, F O; Olugbemi, T S; Ademu, L A

2013-10-01

363

A positioning pillow to improve lumbar puncture success rate in paediatric haematology-oncology patients: a randomized controlled trial  

PubMed Central

Background Lumbar punctures (LPs) are common in children with cancer. Although pain management during the lumbar puncture has been well standardized, dealing with stress and anxiety is not well addressed yet. Our objective was to evaluate the potential improvement of the LP success rate using a positioning pillow, to ensure maximum lumbar flexion, and allow paravertebral muscles to relax, in children who are awake, with either conscious sedation or no sedation. Methods Children aged 2–18 years undergoing LP were randomly assigned to a positioning pillow or no intervention. The primary outcome was the rate of success, i.e. achieving the LP (sampling or injection) at the first attempt, without bleeding (RBC < 50/mm3). The secondary outcomes included: the child's pain, assessed by a self-administered visual analogical scales (VAS) for children over 6 years of age; the parents' and caregivers' perception of the child's pain; the satisfaction of the children, the parents, the caregivers and the physician. The child's cooperation and the occurrence of post-LP syndrome were also evaluated. Results 124 children (62 in each group) were included. The LP pillow tended to increase the success rate of LPs (67% vs. 57%, p = 0.23), and decreased the post-LP syndromes (15% vs. 24%, p = 0.17) but the differences were not statistically significant. In children over 6-year of age (n = 72), the rate of success was significantly higher in the pillow group (58.5% vs. 41.5%, p = 0.031), with a tendency to feel less pain (median VAS 25 vs. 15 mm, p = 0.39) and being more satisfied (84.4% vs. 75.0%, p = 0.34). Conclusion Overall results do not demonstrate a benefit in using this pillow for lumbar punctures. This study results also suggest a benefit in the sub group of children over 6-year of age; this result needs confirmation. Trial Registration The trial was registered with Clinical Trials.gov (number NCT00775112).

2009-01-01

364

Impact of environmental enrichment in mice. 1: Effect of housing conditions on body weight, organ weights and haematology in different strains  

Microsoft Academic Search

Summary Currently, environmental enrichment is a very common means of improving animal well- being, especially for laboratory animals. Although environmental enrichment seems to be a possible way for improving the well-being of animals, the consideration of housing laboratory animals should not only focus solely on animal well-being, manpower and economics but also on the precision and accuracy of the experimental

P.-P. Tsai; U. Pachowsky; H. D. Stelzer; H. Hackbarth

2002-01-01

365

Effect of the plant Cupressus macro-carpa (Cupressacea) on some haematolo-gical and biochemical parameters of Biomphalaria alexandrina snails.  

PubMed

The dry powder of the plant aereal part; Cupressus macro-carpa (Cupressacea) was tested against Biomphalaria alexandrina. LC50 & LC90 values were 59.5 & 98.8 ppm, respec-tively. Exposure of B. alexandrina to sublethal concentrations (LC0, LC10 & LC25) of C. macrocarpa for three weeks signi-ficantly decreased the number of circulating hemocytes. The magnitude of reduction was increased with increasing of the tested concentration. The main type of cell in the hemolymph of B. alexandrina was the granulocyte (71.8%), followed by large round cells or hyalinocytes (19.0%) and small round cells or undifferentiate cells (9.2%). The percentage of different hemocyte categories was changed in treated snails. In snails maintained at LC25, showed significantly higher percentages of small round cells than controls, 56.2% & 9.2% respectively. Maintainence of B. alexandrina in sublethal concentrations for three weeks significantly reduced protein & hemoglobin content in the hemolymph. Reduction in enzyme activities occurred in the hemolymph and tissues of treated snails. The enzymes were pyruvate kinase (PK), lactat dehydrogenase (LDH), hexokinase (HK) and phosphoenol pyruvate carboxy kinase (PEPCK) which are very important in metabolism of the protein and carbohydrate. The infectivity of Schistosoma mansoni miracidia was greatly reduced by exposure to the sublethal concentrations (LC0, LC10 & LC25) of Cupressus. Infection rate of B. alexandrina reached to 54.5%, 37.5% & 16.7%, respectively compared to control (81.25%). Duration of cercarial shedding and the total periodic cercarial production/snail showed significant reduction while the parasite incubation period was significantly longer (p<0.05). PMID:17153702

El-Sayed, Kamelia A

2006-12-01

366

Parenteral nutrition following intensive cytotoxic therapy: an exploratory study on the need for parenteral nutrition after various treatment approaches for haematological malignancies.  

PubMed

Patients receiving intensive cytotoxic therapy are traditionally supported with parenteral nutrition (PN), although it is unclear whether all patients benefit from PN. This study aimed to identify regimen-associated differences in PN requirements, to reveal discrepancies between the number of PN indications and the frequency with which PN was actually given, and to describe characteristics of patients who met nutritional goals without PN. PN indications were defined as: (1) severe malnutrition at admission; (2) a prolonged period (7-10 days) of minimal oral intake; or (3) clinical weight loss >10%. PN was found to be needed in only 35% of consolidation courses, compared with 80% during remission induction and 55% during BMT. Significant differences were also seen between BMT protocols: PN was required in only 37% of autologous BMT recipients conditioned without total body irradiation (for lymphoma) vs 92% of recipients of a mismatched graft. A high body mass index was the only significant characteristic of patients who could do without PN. In conclusion, PN is not required for all patients undergoing intensive cytotoxic therapy. Screening of nutritional status at the start of therapy and monitoring oral intake following cytotoxic treatment may allow more appropriate identification of patients requiring PN. PMID:10338050

Iestra, J A; Fibbe, W E; Zwinderman, A H; Romijn, J A; Kromhout, D

1999-05-01

367

Osmotic, ionic and haematological response of the Port Jackson shark Heterodontus portusjacksoni and the common stingaree Trygonoptera testacea upon exposure to diluted seawater  

Microsoft Academic Search

The osmo-ionoregulatory responses of sub-adult Port Jackson sharks, Heterodontus portusjacksoni (Meyer, 1793), and the stingaree Trygonoptera testacea (Mller and Henle, 1841), were investigated following the transfer from full-strength seawater (100% SW) to either 75 or 50%\\u000a SW for up to 168 h. The plasma osmotic pressure was similarly reduced in both species and remained slightly hyper-osmotic\\u000a to the diluted seawater

A. R. Cooper; S. Morris

1998-01-01

368

Haematological, Biochemical and Coagulation Changes in Mice, Guinea-pigs and Monkeys Infected with a Mouse-adapted Variant of Ebola Zaire Virus  

Microsoft Academic Search

Ebola Zaire virus from the 1976 outbreak (EBO-Z) was recently adapted to the stage of lethal virulence in BALB\\/c mice through serial passage. In the present study, various parameters were examined in groups of mice and guinea-pigs and in three rhesus monkeys after infection with mouse-adapted EBO-Z. The virus caused fatal disease not only in mice but also in guinea-pigs,

M. Bray; S. Hatfill; L. Hensley; J. W. Huggins

2001-01-01

369

The XmnI polymorphic site 5' to the gene G? in a Brazilian patient with sickle cell anaemia - fetal haemoglobin concentration, haematology and clinical features  

PubMed Central

We report a 20-year-old female with sickle cell anaemia and with an HbF concentration of 15.8%. The patient was not using hydroxyurea and was not receiving regular blood transfusions. The patient never had chronic manifestations of sickle cell anaemia, only pain crises of a mild intensity. After laboratory tests, we found that she was homozygous for HbS with the Bantu/atypical haplotype, and was heterozygous for the XmnI site. The influence of the XmnI site on the expression of HbF can explain the amelioration in clinical features in this haplotype association in a case of sickle cell anaemia.

Cancado, Rodolfo D.; Domingos, Claudia R.B.

2010-01-01

370

Evaluation of Biochemical, Haematological, and Histopathological Responses and Recovery Ability of Common Carp (Cyprinus carpio L.) after Acute Exposure to Atrazine Herbicide  

PubMed Central

The aim of study was to evaluate the effect of atrazine exposure (5, 15, 20, and 30?mg·L?1) on common carp and the ability of regeneration. During 96?h exposure we observed abnormal behavior in fish exposed to 20 and 30?mg·L?1. Mortality and histological alterations were noticed only in the group exposed to 30?mg·L?1. Most experimental groups showed significantly (P < 0.05) lower values of haemoglobin, haematocrit, leukocyte, and lymphocyte and significantly higher values of monocytes, segmented and band neutrophile granulocytes, and also metamyelocytes and myelocytes. A significantly lower (P < 0.05) leukocyte count was also recorded in experimental groups (5 and 15?mg·L?1) after recovery period. Statistically significant (P < 0.05) alterations in glucose, total protein, lactate, phosphorus, calcium, and biopterin as well as in activities of ALT, AST, ALP, and LDH were found in most experimental groups. These changes were most apparent in the groups exposed to 20 and 30?mg·L?1. Most of the indices were found to be restored after the 7-day recovery period with the exception of LDH, ALT, and lactate in the group exposed to 15?mg·L?1. Our results showed that atrazine exposure had a profound negative influence on selected indices and also on histological changes of common carp.

Blahova, Jana; Modra, Helena; Sevcikova, Marie; Marsalek, Petr; Zelnickova, Lenka; Skoric, Misa; Svobodova, Zdenka

2014-01-01

371

Effect of low dietary lipid on the development of Sj?gren's syndrome and haematological abnormalities in (NZB x NZW)F1 mice.  

PubMed Central

A diet low in fat was found to retard the development of autoimmune disease in (NZB x NZW)F1 mice, whereas diets high in fat content were associated with more severe disease. The ability of a reduced lipid intake to ameliorate the progression of autoimmune disease was indicated by preserved lacrimal gland secretion (measured by a modified Schirmer test), decreased infiltration of inflammatory cells into the exocrine tissue, and decreased severity of immunohaemolytic anaemia as indicated by near-normal packed cell volume and reticulocyte values. These results suggest that nutritional intervention may be of some help in reducing the severity of pathological abnormalities associated with human systemic lupus erythematosus and Sjögren's syndrome. Images

Swanson, C A; Levy, J A; Morrow, W J

1989-01-01

372

Studies on Parasitologic and Haematologic Activities of an Enaminone Derivative of 4-Hydroxyquinolin-2(1H)-one Against Murine Schistosomiasis Mansoni  

PubMed Central

The activity of a novel enaminone derivative of 4–hydroxyquinoline, BDHQ, was screened for its effectiveness against murine schistosomiasis by electron microscopy and parasitologic studies. The correlation of these studies with serum levels of IFN–gamma and IgE is described. Two groups of 10 mice each were treated with different doses of BDHQ, and their results were correlated with the control and praziquantel (PZQ)–treated groups. Parasitologic study revealed significant reduction in mature worms and tissue egg loads in BDHQ– and PZQ–treated groups, whereas immature worms revealed significant reduction in BDHQ groups only. The group treated with a higher dose of BDHQ showed significant reductions in intestinal ova count when compared with the PZQ–treated group. Ultrastructural examination of the worm revealed significant degeneration of the spines and tegument in all treated groups, while the genital system was affected in BDHQ–treated groups only. BDHQ showed considerable effect on cellular activation where serum levels of IFN–gamma were significantly increased in comparison to control, while anti–soluble worm antigen preparation (SWAP) IgE was significantly increased in comparison to both the control and PZQ–treated groups. Ultrastructural examination revealed cellular activation in buffy coat and the liver in both the BDHQ– and PZQ–treated groups in comparison to the untreated one, whereas in the bone marrow and spleen, evidence of cellular activation was remarkable in the BDHQ–treated groups. In conclusion, BDHQ exhibits high levels of activity against adult and juvenile stages of these parasites, which may be due to its mixed cellular and humoral immunologic mechanisms, as demonstrated by the significant increase of serum levels of IgE and IFN–gamma shown on electron microscopy. Therefore, our results support the comparative advantage that BDHQ has over PZQ.

El-Shennawy, Amal M.; Mohamed, Amira H.; Mohamed Abass

2007-01-01

373

Clinical, haematological and biochemical alterations in heat intolerance (panting) syndrome in Egyptian cattle following natural foot-and-mouth disease (FMD)  

Microsoft Academic Search

Clinical signs of heat intolerance (panting) syndrome were observed in Holstein cows in a private farm in Egypt. There were\\u000a heat intolerance (fever), panting, profuse salivation, hirsutism, lameness and reduced milk production. Blood and serum samples\\u000a were collected from ten diseased cows and five apparently healthy cows as control. Serological tests confirmed the presence\\u000a of non-structural protein of foot-and-mouth disease

Mohamed M. Ghanem; Omnia M. Abdel-Hamid

2010-01-01

374

The selenium accumulation and its effect on growth, and haematological parameters in red sea bream, Pagrus major, exposed to waterborne selenium.  

PubMed

Juvenile Pagrus major (mean length 15.8±1.6cm, and mean weight 90.4±4.7g) were exposed for 4 weeks with waterborne selenium concentration (0, 50, 100, 200, and 400µg/L). The profile of Se accumulation among tissue in P. major is dependent on the exposure periods and Se concentration. After 4 weeks, the highest accumulation of Se was observed in the kidney, and the order of Se accumulation in tissues was kidney?liver>spleen>intestine>gill>muscle. Se decreased the growth rate, and there was an inverse proportion between growth and Se concentration. The major hematological findings were significant decrease in the RBC count, Ht value, and Hb concentration exposed to ?100µg/L Se concentrations. Se exposure (?100µg/L) led to significant increase in the glucose, GOT, and GPT levels, whereas the levels of calcium, magnesium, cholesterol, and total protein did not increase. The results suggest that waterborne Se exposure can induce significant Se accumulation in tissues, inhibition of growth, and hematological alterations. PMID:24636952

Kim, Jun-Hwan; Kang, Ju-Chan

2014-06-01

375

Evaluation of Biochemical, Haematological, and Histopathological Responses and Recovery Ability of Common Carp (Cyprinus carpio L.) after Acute Exposure to Atrazine Herbicide.  

PubMed

The aim of study was to evaluate the effect of atrazine exposure (5, 15, 20, and 30?mg·L(-1)) on common carp and the ability of regeneration. During 96?h exposure we observed abnormal behavior in fish exposed to 20 and 30?mg·L(-1). Mortality and histological alterations were noticed only in the group exposed to 30?mg·L(-1). Most experimental groups showed significantly (P < 0.05) lower values of haemoglobin, haematocrit, leukocyte, and lymphocyte and significantly higher values of monocytes, segmented and band neutrophile granulocytes, and also metamyelocytes and myelocytes. A significantly lower (P < 0.05) leukocyte count was also recorded in experimental groups (5 and 15?mg·L(-1)) after recovery period. Statistically significant (P < 0.05) alterations in glucose, total protein, lactate, phosphorus, calcium, and biopterin as well as in activities of ALT, AST, ALP, and LDH were found in most experimental groups. These changes were most apparent in the groups exposed to 20 and 30?mg·L(-1). Most of the indices were found to be restored after the 7-day recovery period with the exception of LDH, ALT, and lactate in the group exposed to 15?mg·L(-1). Our results showed that atrazine exposure had a profound negative influence on selected indices and also on histological changes of common carp. PMID:24791011

Blahova, Jana; Modra, Helena; Sevcikova, Marie; Marsalek, Petr; Zelnickova, Lenka; Skoric, Misa; Svobodova, Zdenka

2014-01-01

376

Clinical characteristics of 15 children with juvenile myelomonocytic leukaemia who developed blast crisis: MDS Committee of Japanese Society of Paediatric Haematology/Oncology.  

PubMed

Juvenile myelomonocytic leukaemia (JMML) is a rare haematopoietic stem cell disease of early childhood, which can progress to blast crisis in some children. A total of 153 children diagnosed with JMML were reported to the Myelodysplastic Syndrome Committee in Japan between 1989 and 2007; 15 of them (9·8%) had 20% or more blasts in the bone marrow (blast crisis) during the disease course. Blast crisis occurred during observation without therapy (n = 3) or with oral 6-mercaptopurine treatment (n = 9) and in relapse after haematopoietic stem cell transplantation (HSCT; n = 3). Six patients had a complex karyotype (5 including monosomy 7) and an additional three patients had isolated monosomy 7 at blast crisis. Seven patients received HSCT after blast crisis and four of them achieved remission. Eleven out of the 15 patients died; the cause of death was disease progression in 10 patients and transplant-related complication in one patient. In summary, patients with blast crisis have poor prognosis and can be cured only by HSCT. The emergence of monosomy 7 and complex karyotype may be characteristic of blast crisis in a substantial subset of children. PMID:24588411

Honda, Yuko; Tsuchida, Masahiro; Zaike, Yuji; Masunaga, Atsuko; Yoshimi, Ayami; Kojima, Seiji; Ito, Masafumi; Kikuchi, Akira; Nakahata, Tatsutoshi; Manabe, Atsushi

2014-06-01

377

In vivo effects of microcystins and complex cyanobacterial biomass on rats (Rattus norvegicus var. alba): changes in immunological and haematological parameters.  

PubMed

Toxic cyanobacteria represent a serious health and ecological problem in drinking and recreational waters worldwide. Some previous toxicological studies investigated effects of isolated microcystins on laboratory rodents including mice and rats. However, much less attention has been paid to more realistic exposure situations such as the effects of MCs accumulated in food. The objectives of the present study were to provide a simple model simulation of the food chain in order to evaluate impacts of microcystins (MCs) on rat immune and haematologicalparameters. Impacts of feeding experimental rats with a diet containing fish meat with and without microcystins and complex toxic biomass have been studied during a 28 day exposure. Red blood cell parameters (RBC counts, haematocrit values, MCH, MCV and MCHC) showed significant differences in experimental groups (p ? 0.05, p ? 0.01) in comparison with the control group. We also detected an immunomodulatory effect in the experimental groups. NK cells and ??+ T lymphocytes were significantly increased in peripheral blood in the group exposed to isolated microcystin in the food. Significant change in the ratio of CD4+ and CD8+ cells (increase of CD4+ and a drop in CD8+) was found in the group with added cyanobacterial biomass with low concentration of MCs. The greatest changes in lymphoid organs were observed in the same groups. There was an increase of spleen subpopulations of ??+ T lymphocytes as well as of IgM+ lymphocytes (B lymphocytes) and CD8+ T lymphocytes. Indeed, the modulation of CD4+ and CD8+ of peripheral lymphocytes was associated with similar changes in thymic lymphocytic subpopulations. In summary, food containing fish meat with considerable doses of microcystins (or toxic cyanobacterial biomass) induces significant changes in RBC parameters and influence preferably innate part of the immune system represented by NK cells and by gamma-delta T cells, which are known to play role as a bridge between adaptive and innate immune response. PMID:23831285

Palikova, Miroslava; Ondrackova, Petra; Mares, Jan; Adamovsky, Ondrej; Pikula, Jiri; Kohoutek, Jiri; Navratil, Stanislav; Blaha, Ludek; Kopp, Radovan

2013-10-01

378

Cardiac remodelling, blood chemistry, haematology and oxygen consumption of Atlantic cod, Gadus morhua L., induced by experimental haemolytic anaemia with phenylhydrazine  

Microsoft Academic Search

Anaemia is a common pathology associated with many infectious and non-infectious diseases. The effects of haemolytic anaemia\\u000a induced by i.p. injection of phenylhydrazine (PHZ) were studied in Atlantic cod. Phenylhydrazine injection (0.3 mg kg?1) in a DMSO and saline vehicle induced a reproducible and stable anaemia reducing haematocrit, (Hct) by 62% over 3 weeks.\\u000a Controls consisted of fish injected with saline and DMSO\\/saline

Mark D. Powell; Melissa S. Burke; Dalia Dahle

2011-01-01

379

Second malignant neoplasms after cancer in childhood or adolescence. Nordic Society of Paediatric Haematology and Oncology Association of the Nordic Cancer Registries  

Microsoft Academic Search

OBJECTIVE--To assess the relative risk of developing a second malignant neoplasm in people with a diagnosis of cancer in childhood and adolescence. DESIGN--Register based follow up study. SETTING--Populations of Nordic countries. SUBJECTS--30,880 people under the age of 20 with a first malignant neoplasm diagnosed during the period 1943-87. MAIN OUTCOME MEASURES--Relative and attributable risks of second malignant neoplasms by type

J H Olsen; S Garwicz; H Hertz; G Jonmundsson; F Langmark; M Lanning; S O Lie; P J Moe; T Møller; R Sankila

1993-01-01

380

Parenteral nutrition following intensive cytotoxic therapy: an exploratory study on the need for parenteral nutrition after various treatment approaches for haematological malignancies  

Microsoft Academic Search

Patients receiving intensive cytotoxic therapy are traditionally supported with parenteral nutrition (PN), although it is unclear whether all patients benefit from PN. This study aimed to identify regimen-associated differences in PN requirements, to reveal discrepancies between the number of PN indications and the frequency with which PN was actually given, and to describe characteristics of patients who met nutritional goals

JA Iestra; WE Fibbe; AH Zwinderman; JA Romijn; D Kromhout

1999-01-01

381

Galactomannan detection in computerized tomography-based broncho-alveolar lavage fluid and serum in haematological patients at risk for invasive pulmonary aspergillosis  

Microsoft Academic Search

Summary. We determined the value of galactomannan (GM) detection in computerized tomography (CT)-based broncho-alveolar lavage (BAL) fluid and serum for the diagnosis of invasive pulmonary aspergillosis (IPA) in hae- mato-oncological patients with neutropenia. CT of the thorax and BAL were performed systematically at prede- fined clinical indications. GM was determined by sandwich enzyme-linked immunosorbent assay; the clinicians were unaware of

Martin J. Becker; Elly J. Lugtenburg; Jan J. Cornelissen; Cindy van der Schee; Henk C. Hoogsteden; Siem de Marie

2003-01-01

382

Effect of a rosemary and oregano volatile oil mixture on performance, lipid oxidation of meat and haematological parameters in Pharaoh quails  

Microsoft Academic Search

1. This study was conducted to determine the utility of a volatile oil (VO) mixture in quail diets as a natural growth promoter. Different levels of VO mixtures, derived from rosemary and oregano, were added to a basal diet to determine the effects of the mixture on live weight (LW), live weight gain (LWG), feed intake, feed conversion ratio (FCR),

D. Yesilbag; S. S. Gezen; H. Biricik; T. Bulbul

2012-01-01

383

Haematological effects of rhGM-CSF in dogs exposed to total-body irradiation with a dose of 2.4 Gy.  

PubMed

It was the specific aim of this study to test the stimulatory effects of recombinant human GM-CSF (rhGM-CSF) on haemopoietic regeneration in dogs which had received total-body irradiation (TBI) with a dose of 2.4 Gy. In normal dogs rhGM-CSF given subcutaneously at 10 microgram/kg per day or 30 microgram/kg per day for 21 days caused strong but transient increases in the peripheral blood neutrophils. The monocyte counts also showed a transient rise during treatment in a dose-dependent fashion, whereas the lymphocyte counts increased only at the higher dose of rhGM-CSF and the platelet counts were transiently depressed during the course of the treatment. In the irradiated animals treatment with rhGM-CSF decreased the severity and shortened the duration of neutropenia but had no significant influence on monocyte or lymphocyte recovery. The granulocyte values showed a characteristic pattern of fluctuations with the first peak occurring at the same time (day 10 to day 13) when the abortive rise was observed in the untreated dogs. In contrast the GM-CFC in the peripheral blood remained depressed during the whole treatment course, similar to the untreated irradiated controls. These results indicate that treatment with GM-CSF can be an effective biological monotherapy for radiation-induced bone marrow failure, but that for higher radiation doses the number of GM-CSF responsive target cells will become a critical determinant of therapeutic efficacy. PMID:1349334

Nothdurft, W; Selig, C; Fliedner, T M; Hintz-Obertreis, P; Kreja, L; Krumwieh, D; Kurrle, R; Seiler, F R; Weinsheimer, W

1992-04-01

384

Alternative treatment for Anacanthorus penilabiatus (Monogenea: Dactylogyridae) infection in cultivated pacu, Piaractus mesopotamicus (Osteichthyes: Characidae) in Brazil and its haematological effects.  

PubMed

The present work, studied the effect of 0, 1,000, 1,500 and 2,000 mg of garlic powder/kg dry ration for Piaractus mesopotamicus (Osteichthyes: Characidae), weighting 73.6 +/- 39.4 g and measuring 15.0 +/- 2.7 cm, fed for a period of 15, 30 and 45 days. Fifteen days after treatment with 1,000 and 2,000 mg of garlic/kg dry ration, significant reduction of Anacanthorus penilabiatus (Monogenea: Dactylogyridae) in the gills was related. Nevertheless, the addition of garlic to the ration caused significant increase in the erythrocyte number and in the thrombocyte percentage in the circulating blood. However, a decrease in the lymphocyte percentage was also observed. After 45 days, fish fed with garlic showed significant increase in the erythrocyte number, leucocyte, haemoglobin rate, hematocrit and thrombocyte. PMID:12116864

Martins, M L; Moraes, F R; Miyazaki, D M Y; Brum, C D; Onaka, E M; Fenerick, J; Bozzo, F R

2002-06-01

385

Acute effects of chemically dispersed crude oil on gill ion regulation, plasma ion levels and haematological parameters in tambaqui (Colossoma macropomum).  

PubMed

The main goal of this study was to investigate the toxicological effects of the chemical dispersant Corexit 9500, crude oil and the combination of the two components in the form of chemically dispersed crude oil (CO+DIS) on the ion regulation of the tropical fish tambaqui (Colossoma macropomum). Gill ion regulation was evaluated on the basis of unidirectional flux measurements (influx-J(in), efflux-J(out) and net flux-J(net)) of Na(+), Cl(-) and K(+). Plasma ion composition, haematocrit, haemoglobin and glucose concentrations in the blood of tambaqui were determined by classical methods. The exposure of fish to chemically dispersed crude oil promoted a significant increase in J(out) Na(+) across the gills, which, together with the inability of fish to stimulate Na(+) uptake to compensate for these losses resulted in significantly higher J(net) Na(+) outward, particularly within the first 3h of exposure. Increased outward J(net) Cl(-) was also seen in fish that were exposed to dispersed crude oil, whereas outward J(net) K(+) was only increased at crude oil dispersed in higher concentration of Corexit 9500. Plasma Na(+) and Cl(-) concentrations decreased between 6 and 12h of exposure, whereas Ca(2+) concentrations remained significantly lower than those of the control group over the entire experimental period. There were significant increases in plasma K(+) concentrations and in the haematocrit after 6 and 24h of exposure to dispersed crude oil, suggesting significant changes in the permeability of the erythrocytic membrane. Collectively, our results suggest that chemically dispersed crude oil promotes a more extensive impairment of gill ion regulation, in addition to changes in plasma ion levels and blood parameters, in tambaqui compared with exposure to Urucu crude oil or Corexit 9500 alone. Thus, in the event of an oil spill in Amazonian waters, the chemical dispersion of Urucu crude oil could represent a great risk to tambaqui, challenging their ability to maintain ionic and osmotic gradients in native ion-poor waters. PMID:20097436

Duarte, Rafael Mendonça; Honda, Rubens Tomio; Val, Adalberto Luis

2010-04-15

386

Effect of 12-week vanadate and magnesium co-administration on chosen haematological parameters as well as on some indices of iron and copper metabolism and biomarkers of oxidative stress in rats.  

PubMed

Changes in some blood parameters after 12-week administration of sodium metavanadate (SMV; 0.125mgV/ml) or/and magnesium sulphate (MS; 0.06mgMg/ml) in drinking water were studied in outbred male Wistar rats (16 rats/each group) to explore the probable mechanism(s) underlying SMV toxicity and check whether Mg at the level selected during SMV co-administration can protect, at least in part, from a possible deleterious action of SMV. Exposure to SMV alone and in combination with MS (a) led to a decrease in fluid and food intake and body weight gain; (b) predisposed the animals to the development of microcytic-hypochromic anaemia (with excessive liver and spleen Fe deposition, unaltered plasma Fe level and enhanced Zn concentration in the erythrocytes (RBCs) characterized by a reduced haematocrit (Ht) index and haemoglobin (Hb) level, unchanged erythrocyte and reticulocyte count, anisocytosis, lowered total iron binding capacity (TIBC) and elevated transferrin saturation (TS); (c) disturbed Cu homeostasis, but (d) did not influence the leukocyte count and the plasma total antioxidant status (TAS). We suggest that abnormal metabolism and accumulation of Fe as well as an altered Cu status and the RBC Zn level might lead to defective Fe utilization and be a factor promoting the development of Fe-utilization anaemia. The disturbances in the antioxidative capacity reported previously in rats' RBCs after SMV intoxication (?cibior, Zaporowska, Environ. Toxicol. Pharmacol. 30 (2010) 153-161) may suggest that oxidative stress (OS) could also be, in part, involved in the mechanism responsible for the development of anaemia. The Mg dose ingested in combination with V under SMV-MS co-administration (a) was able to decrease, to some extent, the V concentration in the blood, (b) normalized the RBC Mg and Fe levels and (c) restored the values of some parameters of the Fe status near the control values. These results allow a supposition that a higher Mg dose consumed during SMV exposure could have better protective potential and be more effective in limiting the SMV toxicity observed. PMID:22561110

Scibior, Agnieszka; Adamczyk, Agnieszka; Go??biowska, Dorota; Nied?wiecka, Irmina

2012-09-01

387

A three-arm phase III randomised trial assessing, in patients with extensive-disease small-cell lung cancer, accelerated chemotherapy with support of haematological growth factor or oral antibiotics  

PubMed Central

The European Lung Cancer Working Party (ELCWP) designed a 3-arm phase III randomised trial to determine the role of accelerated chemotherapy in extensive-disease (ED) small-cell lung cancer (SCLC). Eligible patients were randomised between the 3 following arms: (A) Standard chemotherapy with 6 courses of EVI (epirubicin 60?mg m?2, vindesine 3?mg m?2, ifosfamide 5?g m?2; all drugs given on day 1 repeated every three weeks. (B) Accelerated chemotherapy with EVI administered every 2 weeks and GM-CSF support. (C) Accelerated chemotherapy with EVI and oral antibiotics (cotrimoxazole). Primary endpoint was survival. 233 eligible patients were randomised. Chemotherapy could be significantly accelerated in arm B with increased absolute dose-intensity. Best response rates, in the population of evaluable patients, were, respectively for arm A, B and C, 59%, 76% and 70%. The response rate was significantly higher in arm B in comparison to arm A (P = 0.04). There was, however, no survival difference with respective median duration and 2-year rate of 286 days and 5% for arm A, 264 days and 6% for arm B and 264 days and 6% for arm C. Severe thrombopenia occurred more frequently in arm B but without an increased rate of bleeding. Non-severe infections were more frequent in arm B and severe infections were less frequent in arm C. Our trial failed to demonstrate, in ED-SCLC, a survival benefit of chemotherapy acceleration by using GM-CSF support. ??http://www.bjcancer.com

Sculier, J P; Paesmans, M; Lecomte, J; Van Cutsem, O; Lafitte, J J; Berghmans, T; Koumakis, G; Florin, M C; Thiriaux, J; Michel, J; Giner, V; Berchier, M C; Mommen, P; Ninane, V; Klastersky, J

2001-01-01

388

Fatal haemoptysis in pulmonary filamentous mycosis: an underevaluated cause of death in patients with acute leukaemia in haematological complete remission. A retrospective study and review of the literature. Gimema Infection Program (Gruppo Italiano Malattie Ematologiche dell'Adulto)  

PubMed

A retrospective study on a consecutive series of 116 patients affected by acute leukaemia with documented pulmonary filamentous mycosis (FM) admitted between 1987 and 1992 to 14 tertiary-care hospitals in Italy was made in order to evaluate the characteristics of those patients who developed fatal massive haemoptysis. In 59/116 cases of pulmonary FM the infection was the principal cause of death and in 12 of these patients a massive haemoptysis was responsible for death. The diagnosis of FM infection was made ante-mortem in only four out of these 12 patients. The autopsy was performed in 11/12 patients and documented a FM infection. The mycetes isolated were: Hyphomycetes spp. (three patients), Mucorales spp. (two patients), Aspergillus spp. (seven patients). At the time of the massive haemoptysis the mean neutrophil count was 7.2 x 10(9)/l, and no patient had relevant thrombocytopenia (mean 184 x 10(9)/l, range 28-350) or coagulative abnormalities. The mean time which elapsed between resolution of chemotherapy-induced neutropenia (WBC < 10(9)/l) and occurrence of haemoptysis was 7 d. No signs or symptoms predictive of this fatal complication were identified. Massive haemoptysis can be the cause of death in patients with acute leukaemia and pulmonary FM which in the majority of patients was not diagnosed in vivo. This complication occurs most frequently shortly after the recovery from chemotherapy-induced aplasia. The mechanism of lesion is unknown, but it may involve the vascular tropism of FM and the release of leucocyte enzymes. Better preventive and therapeutic antifungal treatments are needed to avoid this serious, albeit rare, complication. PMID:7734347

Pagano, L; Ricci, P; Nosari, A; Tonso, A; Buelli, M; Montillo, M; Cudillo, L; Cenacchi, A; Savignana, C; Melillo, L

1995-03-01

389

A comparative study of the toxicity of lead and its impact on the carbohydrate metabolism and some haematological parameters of cichlid fish, oreochromis niloticus and catfish, clarius gariepinus from saudi arabia  

Microsoft Academic Search

Fresh and healthy specimens of Oreochromis niloticus (mean weight 70.5± 1.23 g and mean length, 12.3± 1.5cm) and Clarias gariepinus (mean weight, 41.8±2.3 g and mean length 13.78 ± 1.2 cm) were exposed to heavy metal lead, ranging in concentration from 10 to 18 mg L for O. niloticus and 20 to 28 mg L for C. gariepinus respectively. The

Mohd. Javed Kamal Shamsi

2000-01-01

390

Organisation von Studienteilnahmen in der hämatoonkologischen Praxis  

Microsoft Academic Search

SummaryOrganisation of Study Inclusion in Private Practices for Haematology and OncologyHaemato-oncological studies in private practices for haematology and oncology have been identified as an important instrument for quality assurance in the treatment of haemato-oncological patients. As the organisational structures of private practices for haematology and oncology are usually highly complex the inclusion into studies needs a high motivation and binds

F. Aly; L. Böning; H. Köppler; H. T. Steinmetz; S. Schmitz

2003-01-01

391

[Centralization versus decentralization. Hematology].  

PubMed

Clinical haematology is the result of teamwork among dedicated specialists in pathology, molecular diagnostics, imaging, radiotherapy and the haematologist, who in turn can focus on only a limited fraction of the various and highly complex diseases that together constitute clinical haematology. The treatment of patients should be centralized in departments large enough to permit internal subspecialization and to provide expert service focused on haematology. No more than three such hematology centers are needed in Denmark. PMID:16640979

Borregaard, Niels

2006-04-10

392

High acquisition and environmental contamination rates of CC17 ampicillin-resistant Enterococcus faecium in a Dutch hospital  

Microsoft Academic Search

Methods: In a 3 month study, 210 of 358 admissions (59%) to haematology and gastroenterology\\/ nephrology were screened for rectal ARE colonization on admission (<48 h) and 148 of 210 (70%) also at discharge (<72 h). In a second (3 month) study, environmental swabs from eight predetermined sites were obtained from ARE-colonized haematology patients once weekly. All ARE isolates were

Marieke J. A. de Regt; Lotte E. van der Wagen; Janetta Top; Hetty E. M. Blok; Titia E. M. Hopmans; Adriaan W. Dekker; Ronald J. Hene ´; Peter D. Siersema; Rob J. L. Willems; Marc J. M. Bonten

2008-01-01

393

Fluconazole versus itraconazole for the prevention of fungal infections in haemato-oncology  

Microsoft Academic Search

AIMS: To compare the efficacy of and tolerance to oral fluconazole and intraconazole in preventing fungal infection in neutropenic patients with haematological malignancies. PATIENTS: 213 consecutive, afebrile adult patients treated with or without autologous stem cell transplantation for haematological malignancies. METHODS: A randomised, double blind, single centre study. Patients were randomly assigned to receive fluconazole 50 mg or itraconazole 100

P. C. Huijgens; A. M. Simoons-Smit; A. C. van Loenen; E. Prooy; H. van Tinteren; G. J. Ossenkoppele; A. R. Jonkhoff

1999-01-01

394

Effects of parasitic helminths and ivermectin treatment on clinical parameters in the European wild boar ( Sus scrofa )  

Microsoft Academic Search

Limited information exists on serum biochemistry and haematology of the European wild boar, and few correlations have been found between parasitic burden and clinical parameters in this species. Naturally infected wild boars were experimentally treated to study the effect of nematode parasites and ivermectin treatment on phytohaemagglutinin (PHA) skin reaction and haematological and serum biochemical parameters. White blood cells decreased

Jorge R. López-Olvera; Ursula Höfle; Joaquín Vicente; Isabel G. Fernández-de-Mera; Christian Gortázar

2006-01-01

395

EFFECT OF SUB CHRONIC ADMINISTRATION OF ETHANOLIC LEAF EXTRACT OF CROTON ZAMBESICUS ON HEMATOLOGICAL PARAMETERS OF RATS  

Microsoft Academic Search

Summary: Ethanolic leaf extract of Croton zambesicus was administered to rats at doses of 100 - 400mg \\/ kg for 21 days to investigate its effect on the haematological indices of rats. Haematological indices, namely packed cell volume (PCV), Haemoglobin concentration (Hb) Red blood cell count (RBC), Mean cell Haemoglobin concentration (MCHC), Mean cell volume (MCV), and Mean Corpuscular Haemoglobin

J. E. OKOKON; K. C. IYADI; C. O. EFFIONG

396

EFEITOS DO TRATAMENTO COM ANTI-HELMÍNTICO EM CORDEIROS NATURALMENTE INFECTADOS COM HELMINTOS GASTRINTESTINAIS SOBRE OS PARÂMETROS HEMATOLÓGICOS, GANHO DE PESO E QUALIDADE DA CARCAÇA THE EFFECTS OF ANTHELMINTIC TREATMENT IN LAMBS, NATURALLY INFECTED WITH GASTRINTESTINAL HELMINTHS, ON THE HAEMATOLOGICAL PARAMETERS, WEIGHT GAIN AND CARCASS QUALITY  

Microsoft Academic Search

Sixteen male, crossed-breed lambs were randomly allocated in two groups of 8 animals each. The Group 1 was composed of treated animals with anthelmintic (doramectinÒ) and Group 2 of non- treated animals. The lambs remained with their moth- ers on a pasture of coast-cross (Cynodon dactylon). The lambs were controlled every 14 days by means of eggs counts, packed cell

E. L. KAWANO; M. H. YAMAMURA; E. L. A. RIBEIRO

397

Chronic Industrial Exposure to Lead in 63 Subjects. I. Clinical and Erythrokinetic Findings. II. Evaluation of Chelation Therapy.  

National Technical Information Service (NTIS)

Clinical, biochemical, haematological and erythrokinetic studies were performed on 63 adult males with prolonged lead exposure. Their most common symptoms and findings were abdominal pain (62%), gingival lead lines (48%), headache and/or dizziness (33%), ...

Y. F. Hwang G. T. Strickland N. K. Chang W. M. Beckner R. Q. Blackwell

1976-01-01

398

Haemoglobin H Disease among Chinese Residents of Taiwan.  

National Technical Information Service (NTIS)

Six cases of haemoglobin H disease among 5 Chinese families in Taiwan were studied. The haematological findings resembled those of previous reports and included the occurrence of the electrophoretically fast H haemoglobin component; a slight elevation in ...

K. S. Lin T. C. Lee T. C. Lu J. T. H. Huang R. Q. Blackwell

1965-01-01

399

Stem Cell Transplant Patients and Fungal Infections  

MedlinePLUS

... MA, Donnelly JP. Antifungal prophylaxis during treatment for haematological malignancies: are we there yet? British journal of ... Waterborne, and Environmental Diseases (DFWED) Home A-Z Index Policies Using this Site Link to Us Contact ...

400

ANALYSIS OF THE VOLUME OF RED BLOOD CELLS: APPLICATION OF THE EXPECTATION-MAXIMIZATION ALGORITHM TO GROUPED DATA FROM THE DOUBLY-TRUNCATED LOGNORMAL DISTRIBUTION  

EPA Science Inventory

In accordance with general principles recommended by the International Committee for Standardization in Haematology (1982, Journal of Clinical Pathology 35, 1320-1322), the authors have developed statistical methods for the analysis of red cell volume distributions. To select an ...

401

Parameters of immune and antioxidative systems can be considered as surgical stress markers for gastric cancer patients  

Microsoft Academic Search

Cytokines, haematological indices, lipid peroxidation product and antioxidative system parameters were investigated for surgically treated and red blood cells transfused stages 3 and 4 gastric cancer patients. Serum of 58 patients was analysed at pre-surgical and post-surgical periods (after 7 and 14 days). TGF-beta1 and TNF-alpha were tested by ELISA. Haematological indices were analyzed by standard methods. Malondialdehyde level and

Milasiene Vida; Surinenaite Birute; Prasmickiene Grazina; Didziapetriene Janina; Stratilatovas Eugenijus

2009-01-01

402

Hypertriglyceridaemia and malignancy.  

PubMed

Severe hypertriglyceridaemia is a rare presentation usually associated with acute pancreatitis. We present two case reports of severe hypertriglyceridaemia occurring in patients with previous autoimmune disease and haematological and solid malignancies but no evidence of prior or concurrent pancreatitis or hyperlipidaemia. These case reports illustrate that haematological and solid organ malignancies and autoimmunity may be relevant as exacerbating factors in the presentation of severe type IV or V hypertriglyceridaemia. PMID:15854204

Wijeratne, S; Wray, R; Collinson, P O; Wierzbicki, A S

2005-02-01

403

Persistence of neurological damage induced by dietary vitamin B12 deficiency in infancy  

Microsoft Academic Search

A case is reported of a 14 month old boy with severe dietary vitamin B-12 deficiency caused by his mother’s vegan diet. Cinical, electroencephalography (EEG), and haematological findings are described. Cranial magnetic resonance imaging (MRI) showed severe frontal and frontoparietal cranial atrophy. Vitamin B-12 supplements led to a rapid improvement of haematological and neurological symptoms. Serum vitamin B-12 and urinary

Ursula von Schenck; Christine Bender-Götze; Berthold Koletzko

1997-01-01

404

Early and unexpectedly severe start of influenza epidemic in the Czech Republic during influenza season 2012-13.  

PubMed

A sudden increase in severe influenza has been registered in the Czech Republic since the end of 2012, with 264 cases requiring intensive care, including 51 deaths. Most patients had at least one risk factor. Severe influenza in patients with obesity, smoking and/or haematological disorders including haematological cancers was more frequent than in the pre-pandemic period. The seasonal influenza vaccination status of the cases indicates indirect efficiency of the current vaccine in preventing severe influenza. PMID:23410257

Kyncl, J; Havlickova, M; Nagy, A; Jirincova, H; Piskova, I

2013-01-01

405

Rule based processing of the CD4000, CD3200 and CD Sapphire analyser output using the Cerner Discern Expert Module.  

PubMed

The latest version of our Laboratory Information System haematology laboratory expert system that handles the output of Abbott Cell-Dyn Sapphires, CD4000s and a CD3200 full blood count analyser in three high-volume haematology laboratories is described. The three hospital laboratories use Cerner Millennium Version 2007.02 software and the expert system uses Cerner Millennium Discern Expert rules and some small Cerner Command Language in-house programs. The entire expert system is totally integrated with the area-wide database and has been built and maintained by haematology staff members, as has the haematology database. Using patient demographic data, analyser numeric results, analyser error and morphology flags and previous results for the patient, this expert system decides whether to validate the main full blood count indices and white cell differential, or if the analyser results warrant further operator intervention/investigation before verifying, whether a blood film is required for microscopic review and if abnormal results require phoning to the staff treating the patient. The principles of this expert system can be generalized to different haematology analysers and haematology laboratories that have different workflows and different software. PMID:18691345

Burgess, P; Robin, H; Langshaw, M; Kershaw, G; Pathiraja, R; Yuen, S; Coad, C; Xiros, N; Mansy, G; Coleman, R; Brown, R; Gibson, J; Holman, R; Hubbard, J; Wick, V; Lammers, M; Johnson, R; Huffman, K; Bell, J; Ibrahim, A; Estepa, F; Lovegrove, J; Joshua, D

2009-12-01

406

Effect of dietary Spirulina on reduction of copper toxicity and improvement of growth, blood parameters and phosphatases activities in carp, Cirrhinus mrigala (Hamilton, 1822).  

PubMed

The effect of Spirulina supplementation on reduction of copper toxicity based on food utilization, phosphatases activities and selected haematological parameters was studied in a freshwater cultivable fish C. mrigala. Metal concentration in medium, body tissues and fecal matter was also analysed. Sublethal exposure of C. mrigala fed Spirulina-free diet significantly reduced the consumption and growth rates, phosphatases activities and haematological parameters. However, the above parameters enhanced in the copper exposed fish fed with Spirulina supplemented diets. A significant positive correlation was obtained for the relationship between supplementation of dietary Spirulina and copper defecation through feces. Among the doses of Spirulina supplementation, 6% addition has been considered optimum for C. mrigala since this dose produces maximum elimination of copper from the body and feces and better improvement on growth, phosphatases activities and haematological parameters. PMID:19957889

James, R; Sampath, K; Nagarajan, R; Vellaisamy, P; Manikandan, M Maripandi

2009-09-01

407

Scar sarcoidosis with bone marrow involvement and associated musculoskeletal symptoms  

PubMed Central

Sarcoidosis affects the lungs most commonly and can present with cutaneous lesions. It can also involve the bone marrow in rare instances, often presenting with non-specific symptoms such as fever and malaise, with occasional haematological abnormalities. The authors present the case of a 41-year-old caucasian female who was diagnosed with scar sarcoidosis, but who also reported fatigue, night sweats and polyarthralgia. No haematological abnormalities or lung involvement were detected. A magnetic resonance scan of the spine, performed due to disc prolapses, demonstrated areas of bone oedema and stress response that were reported as a possible sarcoid infiltration of the bone marrow. An improvement occurred after 8 weeks, despite conservative treatment. Bone marrow involvement in sarcoidosis is rarely reported, and was an accidental finding here. Due to the non-specific presentation, cases such as this are potentially underdiagnosed and should be considered in patients with systemic symptoms, regardless of their haematological status.

Hameed, Omair Akhtar; Skibinska, Malgorzata

2011-01-01

408

Altitude exposure in sports: the Athlete Biological Passport standpoint.  

PubMed

The Athlete Biological Passport (ABP) is principally founded on monitoring an athlete's biological variables over time, to identify abnormal biases on a longitudinal basis. Several factors are known to influence the results of these markers. However, the manner in which the altitude factor is taken into account still needs to be standardized. Causal relationships between haematological variables should be correctly integrated into ABP software. In particular, modifications of haematological parameters during and after exposure to different altitudes/hypoxic protocols need to be properly included within detection models. PMID:24115763

Sanchis-Gomar, Fabian; Pareja-Galeano, Helios; Brioche, Thomas; Martinez-Bello, Vladimir; Lippi, Giuseppe

2014-03-01

409

Hepatosplenic candidiasis in the era of new antifungal drugs: a study in Paris 2000-2007.  

PubMed

We report a retrospective study of 24 patients with haematological malignancy and hepatosplenic candidiasis. Clinical and biological features were similar to previous reports. No patient previously received antifungal prophylaxis. Liver or spleen histological examination revealed yeasts in 6/24 patients (25%) on direct examination but all cultures were negative. After a median duration of 7 months, antifungal treatment was discontinued in 58% of the patients with no relapse. Eleven (46%) patients died during follow up. After multivariate analysis, independent factors associated with death were the duration of neutropenia (p 0.022) and relapsing haematological malignancy (p 0.015). PMID:22458883

De Castro, N; Mazoyer, E; Porcher, R; Raffoux, E; Suarez, F; Ribaud, P; Lortholary, O; Molina, J-M

2012-06-01

410

Tyrosine kinase inhibitors and modifications of thyroid function tests: a review  

Microsoft Academic Search

Tyrosine kinase inhibitors (TKI) belong to new molecular multi-targeted therapies that are approved for the treatment of haematological and solid tumours. They interact with a large variety of protein tyrosine kinases involved in oncogenesis. In 2005, the first case of hypothyroidism was described and since then, some data have been published and have confirmed that TKI can affect the thyroid

Frederic Illouz; Sandrine Laboureau-Soares; Vincent Rohmer; Patrice Rodien; CHU d'Angers

2009-01-01

411

Effect of dietary Spirulina on reduction of copper toxicity and improvement of growth, blood parameters and phosphatases activities in carp, Cirrhinus mrigala (Hamilton, 1822)  

Microsoft Academic Search

The effect of Spirulina supplementation on reduction of copper toxicity based on food utilization, phosphatases activities and selected haematological parameters was studied in a freshwater cultivable fish C. mrigala. Metal concentration in medium, body tissues and fecal matter was also analysed. Sublethal exposure of C. mrigala fed Spirulina - free diet significantly reduced the consumption and growth rates, phosphatases activities

R James; K Sampath; R Nagarajan; P. Vellaisamy; M Maripandi Manikandan

412

Lupus érythémateux systémique  

Microsoft Academic Search

The systemic lupus is an autoimmune disease characterized by cutaneous-articular, visceral (kidney, nervous system, serous membranes), and haematological lesions. Due to the various groups of symptoms that have been observed, classification criteria have been proposed; these are useful for cohort comparisons, but are not adequate for individual diagnosis. Grouping main clinical manifestations allows to distinguish benign forms – mainly the

O. Meyer

2005-01-01

413

Increased Withdrawal Volume per Deposit for Pre–Operative Autologous Blood Donation in Adolescents  

Microsoft Academic Search

Objectives: This study was undertaken to assess the feasibility, tolerance, haemodynamic and haematologic effects of an aggressive phlebotomy schedule for autologous blood donation (ABD) in adolescents undergoing major orthopaedic surgery. Methods: Twenty adolescents were studied prospectively; 10 patients in group A donated 20% of the circulating blood volume on 2 occasions, whereas 10 patients in group B donated 10% on

T. Erb; R. Möller; P. Christen; E. Signer; F. J. Frei

2000-01-01

414

Etude Clinique et Symptomatologique Chez le Porc Soumis a Une Irradiation gamma Totale a Dose Letale (Clinical and Symptomatological Study of Pigs Subjected to a Lethal Dose of Integral Gamma Irradiation).  

National Technical Information Service (NTIS)

Results are given of a clinical and haematological study made on a corsican species of pigs wholly exposed to an approximately lethal dose of irradiation. The aim of this work was to examine the changes in the irradiation syndrome of irradiation for pigs ...

M. Vaiman J. L. Guenet J. Maas P. Nizza

1966-01-01

415

Natural killer cells and malignant haemopathies: a model for the interaction of cancer with innate immunity.  

PubMed

Despite recent progress in the therapeutic approach of malignant haemopathies, their prognoses remain frequently poor. Immunotherapy offers an alternative of great interest in this context but defect or abnormal expression of human leukocyte antigens (HLA), frequently observed in cancer cells, limits its efficiency. Natural killer (NK) cells, which are able to kill target cells in a HLA-independent way, represent a novel tool in the treatment of haematological malignancies. Abnormal NK cytolytic function is observed in all the haematological malignancies studied, such as acute leukaemia, myelodysplastic syndromes or chronic myeloid/lymphoid leukaemia. Several mechanisms are involved in the alterations of NK cytotoxicity: decreased expression of activating receptors, increased expression of inhibitory receptors or defective expression of NK ligands on target cells. Further studies are needed to identify how each type of haematological malignancy escapes from the innate immune response. Attempts to increase the expression of activating receptors, to counteract inhibitory receptors expression, or to increase NK cell cytotoxic capacities could overcome tumour escape from innate immunity. These therapies are based on monoclonal antibodies or culture of NK cells in presence of cytokines or dendritic cells. Moreover, many novel drugs used in haematological malignancies [tyrosine kinase inhibitors, IMIDs(®), proteasome inhibitors, demethylating agents, histone deacetylase inhibitors (HDACis), histamine dihydrochloride] display interesting immunomodulatory properties that affect NK cells. These data suggest that combined modalities associating cytotoxic drugs with innate immunity modulators may represent a major breakthrough in tumour eradication. PMID:20697893

Sanchez, C J; Le Treut, T; Boehrer, A; Knoblauch, B; Imbert, J; Olive, D; Costello, R T

2011-01-01

416

The Characterisation of Leukaemias with the Sysmex NE8000  

Microsoft Academic Search

Blood samples from 118 patients with acute and chronic leukaemia and with more than 50% leukaemic cells were processed with the automated haematology analyser Sysmex NE-8000TM. For 92 out of these 118 the differences in the histograms and scattergrams of the NE-8000 could be used in an attempt to characterise the leukaemia. This interpretation of the histograms and scattergrams appeared

Wim van der Meer; Dorine W. Swinkels; Hans L. Willems

1997-01-01

417

Évaluation de l'automate d'hématologie Sysmex SE 9500 ®  

Microsoft Academic Search

The Sysmex SE 9500® evaluation was carried out during three weeks in the Teaching Hospitals in Nice, France. It is a new generation automated haematology analyser performing the complete blood count and differential leukocyte count.It is designed to use electrical impedance, radiofrequency, absorbance spectrophotometry, cytochemical differential and a specific IMI channel for immature granulocytes.The results (linearity, carryover, repeatability, reproductibility and

Laurence Ollier; Karine Maerfeld; Corinne Ferrero-Vacher; Mireille Fraye; Isabelle Sudaka; Danièle Nakul-Aquaronne; Frédéric Berthier; Jacques Bayle

2001-01-01

418

New Red Cell Parameters on the Sysmex XE2100TM as Potential Markers of Functional Iron Deficiency  

Microsoft Academic Search

SummaryBackground:The ideal approach to monitor the haematological response of patients with anaemia associated with chronic renal failure during erythropoietin therapy is controversial. The reticulocyte haemoglobin content (CHr) and percentage hypochromic red cells (% Hypo) may be used as sensitive and specific indicators of functional iron deficiency in clinical situations with otherwise normal red cell indices. In the reticulocyte channel of

C. Briggs; R. Rogers; B. Thompson; S. J. Machin

2001-01-01

419

Tolerance of Ciprofloxacin at Injection Site, Systemic Safety and Effect on Electroencephalogram  

Microsoft Academic Search

The safety of ciprofloxacin, given via 30- and 60-min intravenous infusions at a dose of 300 mg every 12 h for 4 days, was studied in 12 healthy subjects (6 females, 6 males). Local effects of the drug were assessed by frequent examination at the infusion site while systemic safety was determined by haematological and biochemical tests and by careful

Sigurdur B. Thorsteinsson; Tom Bergan; Gudjon Johannesson; Hrund S. Thorsteinsson; Rolf Rohwedder

1987-01-01

420

A rapid high-precision flow cytometry based technique for total white blood cell counting in chickens  

Microsoft Academic Search

The automated analysis of total white blood cell count and white blood cell differentials is routine in research and clinical diagnosis in mammalian species. In contrast, in avian haematology these parameters are still estimated by conventional microscopic procedures due to technical difficulties associated with the morphological peculiarities of avian erythrocytes and thrombocytes. Both cell types are nucleated and fairly resistant

Christian Seliger; Beatrice Schaerer; Marina Kohn; Helene Pendl; Steffen Weigend; Bernd Kaspers; Sonja Härtle

421

Automated counting of nucleated red blood cells in blood samples of newborns  

Microsoft Academic Search

Summary Nucleated red blood cells (NRBC) in blood samples interfere with the white blood cell (WBC) count on many types of automated haematology analysers. This makes it necessary to correct the WBC count by counting NRBC microscopically. This report describes the evaluation of two analysers, the Cell-Dyn 4000 and the Sysmex XE-2100, which use new techniques to recognize and enumerate

M. H. De Keijzer; W. Van Der Meer

2002-01-01