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Sample records for helium radiation damage

  1. Interrogating the Effects of Radiation Damage Annealing on Helium Diffusion Kinetics in Apatite

    NASA Astrophysics Data System (ADS)

    Willett, C. D.; Fox, M.; Shuster, D. L.

    2015-12-01

    Apatite (U-Th)/He thermochronology is commonly used to study landscape evolution and potential links between climate, erosion and tectonics. The technique relies on a quantitative understanding of (i) helium diffusion kinetics in apatite, (ii) an evolving 4He concentration, (iii) accumulating damage to the crystal lattice caused by radioactive decay[1], and (iv) the thermal annealing of such damage[2],[3], which are each functions of both time and temperature. Uncertainty in existing models of helium diffusion kinetics has resulted in conflicting conclusions, especially in settings involving burial heating through geologic time. The effects of alpha recoil damage annealing are currently assumed to follow the kinetics of fission track annealing (e.g., reference [3]), although this assumption is difficult to fully validate. Here, we present results of modeling exercises and a suite of experiments designed to interrogate the effects of damage annealing on He diffusivity in apatite that are independent of empirical calibrations of fission track annealing. We use the existing experimental results for Durango apatite[2] to develop and calibrate a new function that predicts the effects of annealing temperature and duration on measured diffusivity. We also present a suite of experiments conducted on apatite from Sierra Nevada, CA granite to establish whether apatites with different chemical compositions have the same behavior as Durango apatite. Crystals were heated under vacuum to temperatures between 250 and 500°C for 1, 10, or 100 hours. The samples were then irradiated with ~220 MeV protons to produce spallogenic 3He, the diffusant then used in step-heating diffusion experiments. We compare the results of these experiments and model calibrations to existing models. Citations: [1]Shuster, D., Flowers R., and Farley K., (2006), EPSL 249(3-4), 148-161; [2]Shuster, D. and Farley, K., (2009), GCA 73 (1), 6183-6196; [3]Flowers, R., Ketcham, R., Shuster, D. and Farley, K

  2. Modeling radiation damage near grain boundary in helium-doped α-iron

    SciTech Connect

    Xu, C. P.; Liu, X. -Y.; Gao, F.; Li, Y. H.; Wang, Y. Q.

    2014-08-01

    Molecular dynamics (MD) simulations are performed to investigate how Σ3<110> (121) symmetric tilt grain boundary (GB) affects point defects and defect clusters in He-doped α-iron at 300 K in picosecond time scales. Molecular statics calculations are also performed and show that the formation energy is reduced in the GB, and the GB acts as a good sink for point defects especially for interstitial He and self-interstitial atoms (SIAs). It is observed that the average size of HenVm (m>n) clusters becomes smaller in the GB-containing Fe system. It is also found that the number of HenV (n=2, 3) clusters in the GB region decreases, while the number of the HeV clusters increases. The GBs loaded with substitutional or interstitial helium atoms are found to facilitate the growth of helium clusters in the GB region.

  3. Modeling radiation damage near grain boundary in helium-doped α-iron

    NASA Astrophysics Data System (ADS)

    Xu, C. P.; Liu, X.-Y.; Gao, F.; Li, Y. H.; Wang, Y. Q.

    2014-08-01

    Molecular dynamics (MD) simulations are performed to investigate how ∑3<1 1 0>(1 2 1) symmetric tilt grain boundary (GB) affects point defects and defect clusters in He-doped α-iron at 300 K in picosecond time scales. Molecular statics calculations are also performed and show that the formation energy is reduced in the GB, and the GB acts as a good sink for point defects, especially for interstitial He and self-interstitial atoms (SIAs). It is observed that the average size of HenVm (m > n) clusters becomes smaller in the GB-containing Fe system, where m and n represent the number of vacancies and He atoms in the cluster, respectively. It is also found that the number of HenV (n = 2, 3) clusters in the GB region decreases, while the number of the HeV clusters increases. The GBs loaded with substitutional or interstitial helium atoms are found to facilitate the growth of helium clusters in the GB region.

  4. Radiation source for helium magnetometers

    NASA Technical Reports Server (NTRS)

    Slocum, Robert E. (Inventor)

    1991-01-01

    A radiation source (12) for optical magnetometers (10) which use helium isotopes as the resonance element (30) includes an electronically pumped semiconductor laser (12) which produces a single narrow line of radiation which is frequency stabilized to the center frequency of the helium resonance line to be optically pumped. The frequency stabilization is accomplished using electronic feedback (34, 40, 42, 44) to control a current sources (20) thus eliminating the need for mechanical frequency tuning.

  5. Helium vs. Proton Induced Displacement Damage in Electronic Materials

    NASA Technical Reports Server (NTRS)

    Ringo, Sawnese; Barghouty, A. F.

    2010-01-01

    In this project, the specific effects of displacement damage due to the passage of protons and helium nuclei on some typical electronic materials will be evaluated and contrasted. As the electronic material absorbs the energetic proton and helium momentum, degradation of performance occurs, eventually leading to overall failure. Helium nuclei traveling at the same speed as protons are expected to impart more to the material displacement damage; due to the larger mass, and thus momentum, of helium nuclei compared to protons. Damage due to displacement of atoms in their crystalline structure can change the physical properties and hence performance of the electronic materials.

  6. A review of helium-hydrogen synergistic effects in radiation damage observed in fusion energy steels and an interaction model to guide future understanding

    NASA Astrophysics Data System (ADS)

    Marian, Jaime; Hoang, Tuan; Fluss, Michael; Hsiung, Luke L.

    2015-07-01

    Under fusion reactor conditions, large quantities of irradiation defects and transmutation gases are produced per unit time by neutrons, resulting in accelerated degradation of structural candidate ferritic (F) and ferritic/martensitic (F/M) steels. Due to the lack of a suitable fusion neutron testing facility, we must rely on high-dose-rate ion-beam experiments and present-day crude modeling estimates. Of particular interest is the possibility of synergistic (positive feedback) effects on materials properties due to the simultaneous action of He, H, and displacement damage (dpa) during operation. In this paper we discuss the state-of-the-art in terms of the experimental understanding of synergistic effects and carry out simulations of triple-species irradiation under ion-beam conditions using first-of-its-kind modeling techniques. Although, state-of-the-art modeling and simulation is not sufficiently well developed to shed light on the experimental uncertainties, we are able to conclude that it is not clear whether synergistic effects, the evidence of which is still not conclusive, will ultimately play a critical role in material performance under fusion energy conditions. We review here some of the evidence for the synergistic effects of hydrogen in the presence of helium and displacement damage, and also include some recent data from our research. While the experimental results to date suggest possible mechanisms for the observed synergistic effects, it is only with more advanced modeling that we can hope to understand the details underlying the experimental observations. By employing modeling and simulation we propose an interaction model that is qualitatively consistent with experimental observations of dpa/He/H irradiation behavior. Our modeling, the results of which should be helpful to researchers going forward, points to gaps and voids in the current understanding of triple ion-beam irradiation effects (displacement damage produced simultaneously with

  7. Krypton and helium irradiation damage in neodymium-zirconolite

    NASA Astrophysics Data System (ADS)

    Gilbert, M.; Davoisne, C.; Stennett, M.; Hyatt, N.; Peng, N.; Jeynes, C.; Lee, W. E.

    2011-09-01

    A leading candidate for the immobilisation of actinides, zirconolite's suitability as a potential ceramic host for plutonium disposition, both in storage and geological disposal, has been the subject of much research. One key aim of this study is to understand the effects of radiation damage and noble gas accommodation within the zirconolite material. To this end, a series of ex situ irradiations have been performed on polycrystalline (Ca 0.8Nd 0.2)Zr(Ti 1.8Al 0.2)O 7 zirconolite samples. Zirconolite samples, doped with Nd 3+ (as a Pu surrogate) on the Ca-site and charge-balanced by substituting Al 3+ onto the Ti-site, were irradiated with 36Kr + (2 MeV) ions at fluences of 1 × 10 14 and 5 × 10 15 cm -2 and 4He + (200 keV) ions at fluences of 1 × 10 14, 5 × 10 15 and 1 × 10 17 cm -2 to simulate the impact of alpha decay on the microstructure. Microstructural analysis revealed no damage present at the lower Kr + fluence, but that the higher 36Kr + fluence rendered the zirconolite completely amorphous. Similarly, evidence of helium accumulation was only seen at the highest 4He + fluence (1 × 10 17 cm -2). Monte Carlo simulations using the TRIM code predict the highest concentration of helium accumulating at a depth of 720 nm, in good agreement with the experimental observations.

  8. Modifying Radiation Damage

    PubMed Central

    Kim, Kwanghee; McBride, William H.

    2011-01-01

    Radiation leaves a fairly characteristic footprint in biological materials, but this is rapidly all but obliterated by the canonical biological responses to the radiation damage. The innate immune recognition systems that sense “danger” through direct radiation damage and through associated collateral damage set in motion a chain of events that, in a tissue compromised by radiation, often unwittingly result in oscillating waves of molecular and cellular responses as tissues attempt to heal. Understanding “nature’s whispers” that inform on these processes will lead to novel forms of intervention targeted more precisely towards modifying them in an appropriate and timely fashion so as to improve the healing process and prevent or mitigate the development of acute and late effects of normal tissue radiation damage, whether it be accidental, as a result of a terrorist incident, or of therapeutic treatment of cancer. Here we attempt to discuss some of the non-free radical scavenging mechanisms that modify radiation responses and comment on where we see them within a conceptual framework of an evolving radiation-induced lesion. PMID:20583981

  9. Radiation Damage Workshop

    NASA Technical Reports Server (NTRS)

    Stella, P. M.

    1984-01-01

    The availability of data regarding the radiation behavior of GaAs and silicon solar cells is discussed as well as efforts to provide sufficient information. Other materials are considered too immature for reasonable radiation evaluation. The lack of concern over the possible catastrophic radiation degradation in cascade cells is a potentially serious problem. Lithium counterdoping shows potential for removing damage in irradiated P-type material, although initial efficiencies are not comparable to current state of the art. The possibility of refining the lithium doping method to maintain high initial efficiencies and combining it with radiation tolerant structures such as thin BSF cells or vertical junction cells could provide a substantial improvement in EOL efficiencies. Laser annealing of junctions, either those formed ion implantation or diffusion, may not only improve initial cell performance but might also reduce the radiation degradation rate.

  10. Influence of displacement damage on deuterium and helium retention in austenitic and ferritic-martensitic alloys considered for ADS service

    NASA Astrophysics Data System (ADS)

    Voyevodin, V. N.; Karpov, S. A.; Kopanets, I. E.; Ruzhytskyi, V. V.; Tolstolutskaya, G. D.; Garner, F. A.

    2016-01-01

    The behavior of ion-implanted hydrogen (deuterium) and helium in austenitic 18Cr10NiTi stainless steel, EI-852 ferritic steel and ferritic/martensitic steel EP-450 and their interaction with displacement damage were investigated. Energetic argon irradiation was used to produce displacement damage and bubble formation to simulate nuclear power environments. The influence of damage morphology and the features of radiation-induced defects on deuterium and helium trapping in structural alloys was studied using ion implantation, the nuclear reaction D(3He,p)4He, thermal desorption spectrometry and transmission electron microscopy. It was found in the case of helium irradiation that various kinds of helium-radiation defect complexes are formed in the implanted layer that lead to a more complicated spectra of thermal desorption. Additional small changes in the helium spectra after irradiation with argon ions to a dose of ≤25 dpa show that the binding energy of helium with these traps is weakly dependent on the displacement damage. It was established that retention of deuterium in ferritic and ferritic-martensitic alloys is three times less than in austenitic steel at damage of ˜1 dpa. The retention of deuterium in steels is strongly enhanced by presence of radiation damages created by argon ion irradiation, with a shift in the hydrogen release temperature interval of 200 K to higher temperature. At elevated temperatures of irradiation the efficiency of deuterium trapping is reduced by two orders of magnitude.

  11. The expected radiation damage of CSNS target

    NASA Astrophysics Data System (ADS)

    Yin, W.; Yu, Q. Z.; Lu, Y. L.; Wang, S. L.; Tong, J. F.; Liang, T. J.

    2012-12-01

    The radiation damage to the tungsten target and its SS316 vessel for Chinese Spallation Neutron Source (CSNS) has been estimated with a Monte-Carlo simulation code MCNPX2.5.0. We compare the effects on the radiation damage due to two different proton beam profiles: a uniform distribution and a Gaussian distribution. We also discuss the dependence of the radiation damage estimation on different physics models. The results show the peak displacement productions in vessel and the fourth target plate are 2.5 and 5.5 dpa/y, respectively, under a Gaussian proton beam. The peak helium productions in the vessel and the fourth target are 305 and 353 appm/y, respectively, under the same proton beam. Based on these results and the allowable dpa values we have estimated the lifetime of the tungsten target and its vessel.

  12. Blackbody-radiation correction to the polarizability of helium

    SciTech Connect

    Puchalski, M.; Jentschura, U. D.; Mohr, P. J.

    2011-04-15

    The correction to the polarizability of helium due to blackbody radiation is calculated near room temperature. A precise theoretical determination of the blackbody radiation correction to the polarizability of helium is essential for dielectric gas thermometry and for the determination of the Boltzmann constant. We find that the correction, for not too high temperature, is roughly proportional to a modified hyperpolarizability (two-color hyperpolarizability), which is different from the ordinary hyperpolarizability of helium. Our explicit calculations provide a definite numerical result for the effect and indicate that the effect of blackbody radiation can be excluded as a limiting factor for dielectric gas thermometry using helium or argon.

  13. Synergistic Effect of Triple Ion Beams on Radiation Damage in CLAM Steel

    NASA Astrophysics Data System (ADS)

    Yuan, Da-Qing; Zheng, Yong-Nan; Zuo, Yi; Fan, Ping; Zhou, Dong-Mei; Zhang, Qiao-Li; Ma, Xiao-Qiang; Cui, Bao-Qun; Chen, Li-Hua; Jiang, Wei-Sheng; Wu, Yi-Can; Huang, Qun-Ying; Peng, Lei; Cao, Xing-Zhong; Wang, Bao-Yi; Wei, Long; Zhu, Sheng-Yun

    2014-04-01

    The synergistic effect of triple ion beams is investigated by simultaneous and sequential irradiations of gold, hydrogen and helium ions on the low activation martensitic steel (CLAM) developed in China. The depth profile measurements of the positron annihilation Doppler broadening S parameter are carried out as a function of slow-positron beam energy to examine the produced radiation damage. The synergistic effect of displacement damage and hydrogen and helium on the formation of radiation damage is clearly observed. In the preset case, this effect suppresses the radiation damage in the CLAM steel due to the helium and/or hydrogen filling of vacancy clusters.

  14. Plasmid DNA damage induced by helium atmospheric pressure plasma jet

    NASA Astrophysics Data System (ADS)

    Han, Xu; Cantrell, William A.; Escobar, Erika E.; Ptasinska, Sylwia

    2014-03-01

    A helium atmospheric pressure plasma jet (APPJ) is applied to induce damage to aqueous plasmid DNA. The resulting fractions of the DNA conformers, which indicate intact molecules or DNA with single- or double-strand breaks, are determined using agarose gel electrophoresis. The DNA strand breaks increase with a decrease in the distance between the APPJ and DNA samples under two working conditions of the plasma source with different parameters of applied electric pulses. The damage level induced in the plasmid DNA is also enhanced with increased plasma irradiation time. The reactive species generated in the APPJ are characterized by optical emission spectra, and their roles in possible DNA damage processes occurring in an aqueous environment are also discussed.

  15. Helium Bubble Injection Solution To The Cavitation Damage At The Spallation Neutron Source

    NASA Astrophysics Data System (ADS)

    Francis, M. W.; Ruggles, A. E.

    2009-03-01

    The Spallation Neutron Source (SNS) is one of the largest science projects in the United States, with total cost near 1.4 Billion Dollars. The limiting factor of the facility had always been assumed to be the lifetime of the target window due to radiation damage. After further investigation, the lifetime of the target was determined not to be limited by radiation damage but by cavitation damage. The cavitation damage derives from pressure waves caused by the beam energy deposition. Vapor bubbles form when low to negative pressures occur in the mercury near the stainless steel target window due to wave interaction with the structure. Collapse of these bubbles can focus wave energy in small liquid jets that erode the window surface. Compressibility of the mercury can be enhanced to reduce the amplitude of the pressure wave caused by the beam energy deposition. To enhance compressibility, small (10 to 30 micron diameter) gas bubbles could be injected into the bulk of the mercury. Solubility and diffusivity parameters of inert gas in mercury are required for a complete mechanical simulation and engineering of these strategies. Using current theoretical models, one obtains a theoretical Henry coefficient of helium in mercury on the order of 3.9E15 Pa-molHg/molHe at 300 K. This low solubility was confirmed by a direct, offline experimental method. Mercury was charged with helium and any pressure change was recorded. Any pressure change was attributed to gas going into solution. Therefore, with the sensitivity of the experiment, a lower limit of 9E12 Pa-molHg/molHe was placed on the mercury-helium system. These values guarantee a stable bubble lifetime needed within the SNS mercury target to mitigate cavitation issues.

  16. Helium Bubble Injection Solution To The Cavitation Damage At The Spallation Neutron Source

    SciTech Connect

    Francis, M. W.; Ruggles, A. E.

    2009-03-10

    The Spallation Neutron Source (SNS) is one of the largest science projects in the United States, with total cost near 1.4 Billion Dollars. The limiting factor of the facility had always been assumed to be the lifetime of the target window due to radiation damage. After further investigation, the lifetime of the target was determined not to be limited by radiation damage but by cavitation damage. The cavitation damage derives from pressure waves caused by the beam energy deposition. Vapor bubbles form when low to negative pressures occur in the mercury near the stainless steel target window due to wave interaction with the structure. Collapse of these bubbles can focus wave energy in small liquid jets that erode the window surface. Compressibility of the mercury can be enhanced to reduce the amplitude of the pressure wave caused by the beam energy deposition. To enhance compressibility, small (10 to 30 micron diameter) gas bubbles could be injected into the bulk of the mercury. Solubility and diffusivity parameters of inert gas in mercury are required for a complete mechanical simulation and engineering of these strategies. Using current theoretical models, one obtains a theoretical Henry coefficient of helium in mercury on the order of 3.9E15 Pa-molHg/molHe at 300 K. This low solubility was confirmed by a direct, offline experimental method. Mercury was charged with helium and any pressure change was recorded. Any pressure change was attributed to gas going into solution. Therefore, with the sensitivity of the experiment, a lower limit of 9E12 Pa-molHg/molHe was placed on the mercury-helium system. These values guarantee a stable bubble lifetime needed within the SNS mercury target to mitigate cavitation issues.

  17. Chemical Protection Against Radiation Damage

    ERIC Educational Resources Information Center

    Campaigne, Ernest

    1969-01-01

    Discusses potential war time and medical uses for chemical compounds giving protection against radiation damage. Describes compounds known to protect, research aimed at discovering such compounds, and problems of toxicity. (EB)

  18. Nuclear Radiation Damages Minds!

    ERIC Educational Resources Information Center

    Blai, Boris, Jr.

    Professors Ernest Sternglass (University of Pittsburgh) and Steven Bell (Berry College) have assembled cogent, conclusive evidence indicating that nuclear radiation is associated with impaired cognition. They suggest that Scholastic Aptitude Scores (SATs), which have declined steadily for 19 years, will begin to rise. Their prediction is based on…

  19. Radiation damage evolution in ceramics

    SciTech Connect

    Devanathan, Ramaswami

    2009-09-15

    A review is presented of recent results on radiation damage production, defect accumulation and dynamic annealing in a number of ceramics, such as silicon carbide, zircon and zirconia. Under energetic particle irradiation, ceramics can undergo amorphization by the accumulation of point defects and defect clusters (silicon carbide) or direct impact amorphization (zircon). Ceramics that resist radiation-induced amorphization have mechanisms to dissipate the primary knock-on atom energy, such as replacement collision sequences that leave the lattice undisturbed and low-energy cation site exchange. The presence of engineered mobile defects, such as structural vacancies in stabilized zirconia, can dynamically anneal radiation damage. Thus, defect engineering is a promising strategy to design radiation tolerance for applications such as nuclear waste disposal.

  20. STS-118 Radiator Impact Damage

    NASA Technical Reports Server (NTRS)

    Lear, Dana M.; Hyde, J.; Christiansen, E.; Herrin, J.; Lyons, F.

    2008-01-01

    During the August 2007 STS-118 mission to the International Space Station, a micro-meteoroid or orbital debris (MMOD) particle impacted and completely penetrated one of shuttle Endeavour s radiator panels and the underlying thermal control system (TCS) blanket, leaving deposits on (but no damage to) the payload bay door. While it is not unusual for shuttle orbiters to be impacted by small MMOD particles, the damage from this impact is larger than any previously seen on the shuttle radiator panels. A close-up photograph of the radiator impact entry hole is shown in Figure 1, and the location of the impact on Endeavour s left-side aft-most radiator panel is shown in Figure 2. The aft radiator panel is 0.5-inches thick and consists of 0.011 inch thick aluminum facesheets on the front and back of an aluminum honeycomb core. The front facesheet is additionally covered by a 0.005 inch thick layer of silver-Teflon thermal tape. The entry hole in the silver-Teflon tape measured 8.1 mm by 6.4 mm (0.32 inches by 0.25 inches). The entry hole in the outer facesheet measured 7.4 mm by 5.3 mm (0.29 inches by 0.21 inches) (0.23 inches). The impactor also perforated an existing 0.012 inch doubler that had been bonded over the facesheet to repair previous impact damage (an example that lightning can strike the same place twice, even for MMOD impact). The peeled-back edge around the entry hole, or lip , is a characteristic of many hypervelocity impacts. High velocity impact with the front facesheet fragmented the impacting particle and caused it to spread out into a debris cloud. The debris cloud caused considerable damage to the internal honeycomb core with 23 honeycomb cells over a region of 28 mm by 26 mm (1.1 inches by 1.0 inches) having either been completely destroyed or partially damaged. Figure 3 is a view of the exit hole in the rear facesheet, and partially shows the extent of the honeycomb core damage and clearly shows the jagged petaled exit hole through the backside

  1. Radiation damage of germanium detectors

    NASA Technical Reports Server (NTRS)

    Pehl, R. H.

    1978-01-01

    Energetic particles can produce interstitial-vacancy pairs in a crystal by knocking the atoms from their normal positions. Detectors are unique among semiconductor devices in depending on very low concentrations of electrically active impurities, and also on efficient transport of holes and electrons over relatively large distances. Because the dense regions of damage produced by energetic particles may result in donors and/or acceptors, and also provide trapping sites for holes and electrons, detectors are very sensitive to radiation damage. In addition to these effects occurring within the detector, radiation may also change the characteristics of the exposed surfaces causing unpredictable effects on the detector leakage current. Radiation-induced surface degradation has rarely, if ever, been observed for germanium detectors. The possibility of minimizing hole trapping in charge collection by the use of a high-purity germanium coaxial detector configured with the p (+) contact on the coaxial periphery is discussed.

  2. Damage thresholds for terahertz radiation

    NASA Astrophysics Data System (ADS)

    Dalzell, Danielle R.; McQuade, Jill; Vincelette, Rebecca; Ibey, Bennet; Payne, Jason; Thomas, Robert; Roach, W. P.; Roth, Caleb L.; Wilmink, Gerald J.

    2010-02-01

    Several international organizations establish minimum safety standards to ensure that workers and the general population are protected against adverse health effects associated with electromagnetic radiation. Suitable standards are typically defined using published experimental data. To date, few experimental studies have been conducted at Terahertz (THz) frequencies, and as a result, current THz standards have been defined using extrapolated estimates from neighboring spectral regions. In this study, we used computational modeling and experimental approaches to determine tissue-damage thresholds at THz frequencies. For the computational modeling efforts, we used the Arrhenius damage integral to predict damage-thresholds. We determined thresholds experimentally for both long (minutes) and short (seconds) THz exposures. For the long exposure studies, we used an in-house molecular gas THz laser (υ= 1.89 THz, 189.92 mW/cm2, 10 minutes) and excised porcine skin. For the short exposure studies, we used the Free Electron Laser (FEL) at Jefferson Laboratory (υ= 0.1-1.0 THz, 2.0-14.0 mW/cm2, 2 seconds) and wet chamois cloths. Thresholds were determined using conventional damage score determination and probit analysis techniques, and tissue temperatures were measured using infrared thermographic techniques. We found that the FEL was ideal for tissue damage studies, while our in-house THz source was not suitable to determine tissue damage thresholds. Using experimental data, the tissue damage threshold (ED50) was determined to be 7.16 W/cm2. This value was in well agreement with that predicted using our computational models. We hope that knowledge of tissue-damage thresholds at THz frequencies helps to ensure the safe use of THz radiation.

  3. Extreme ultraviolet radiation emitted by helium microwave driven plasmas

    NASA Astrophysics Data System (ADS)

    Espinho, S.; Felizardo, E.; Tatarova, E.; Alves, L. L.

    2016-06-01

    The extreme ultraviolet radiation emitted by helium microwave-driven (2.45 GHz) plasmas operating at low-pressure conditions was investigated. Novel data regarding emitted spectral lines of excited helium atoms and ions in the 20-33 nm wavelength range and their intensity behavior with variation of discharge operational conditions are presented. The intensity of all the spectral emissions was found to strongly increase with the microwave power delivered to the plasma. Furthermore, the intensity of the ionic spectral emissions decreases by nearly one order of magnitude as the pressure was raised from 0.2 to 0.5 mbar.

  4. Primordial helium and the cosmic background radiation

    SciTech Connect

    Steigman, Gary

    2010-04-01

    The products of primordial nucleosynthesis, along with the cosmic microwave background (CMB) photons, are relics from the early evolution of the Universe whose observations probe the standard model of cosmology and provide windows on new physics beyond the standard models of cosmology and of particle physics. According to the standard, hot big bang cosmology, long before any stars have formed a significant fraction ( ∼ 25%) of the baryonic mass in the Universe should be in the form of helium-4 nuclei. Since current observations of {sup 4}He are restricted to low redshift regions where stellar nucleosynthesis has occurred, an observation of high redshift, prestellar, truly primordial {sup 4}He would constitute a fundamental test of the hot, big bang cosmology. At recombination, long after big bang nucleosynthesis (BBN) has ended, the temperature anisotropy spectrum imprinted on the CMB depends on the {sup 4}He abundance through its connection to the electron density and the effect of the electron density on Silk damping. Since the relic abundance of {sup 4}He is relatively insensitive to the universal density of baryons, but is sensitive to a non-standard, early Universe expansion rate, the primordial mass fraction of {sup 4}He, Yp, offers a test of the consistency of the standard models of BBN and the CMB and, provides constraints on non-standard physics. Here, the WMAP seven year data (supplemented by other CMB experiments), which lead to an indirect determination of Yp at high redshift, are compared to the BBN predictions and to the independent, direct observations of {sup 4}He in low redshift, extragalactic HII regions. At present, given the very large uncertainties in the CMB-determined primordial {sup 4}He abundance (as well as for the helium abundances inferred from HII region observations), any differences between the BBN predictions and the CMB observations are small, at a level ∼<1.5σ.

  5. Method and means of reducing erosion of components of plasma devices exposed to helium and hydrogen isotope radiation

    DOEpatents

    Kaminsky, Manfred S.; Das, Santosh K.; Rossing, Thomas D.

    1977-01-25

    Surfaces of components of plasma devices exposed to radiation by atoms or ions of helium or isotopes of hydrogen can be protected from damage due to blistering by shielding the surfaces with a structure formed by sintering a powder of aluminum or beryllium and its oxide or by coating the surfaces with such a sintered metal powder.

  6. Protein damage, radiation sensitivity and aging.

    PubMed

    Radman, Miroslav

    2016-08-01

    This paper promotes a concept that protein damage determines radiation resistance and underlies aging and age-related diseases. The first bottleneck in cell recovery from radiation damage is functional (proteome) rather than informational (DNA), since prokaryotic and eukaryotic cell death correlates with incurred protein, but not DNA, damage. Proteome protection against oxidative damage determines survival after ionizing or UV irradiation, since sufficient residual proteome activity is required to turn on the DNA damage response activating DNA repair and protein renewal processes. Extreme radiation and desiccation resistance of rare bacterial and animal species is accounted for by exceptional constitutive proteome protection against oxidative damage. After excessive radiation their well-protected proteome faithfully reconstitutes a transcription-competent genome from hundreds of DNA fragments. The observation that oxidative damage targeted selectively to cellular proteins results in aging-like phenotypes suggests that aging and age-related diseases could be phenotypic consequences of proteome damage patterns progressing with age. PMID:27264559

  7. Radiation damage effects in zircon

    NASA Astrophysics Data System (ADS)

    Trachenko, Kostya; Dove, Martin; Salje, Ekhard

    2002-03-01

    Zircon, ZrSiO_4, is important for geology and geochronology, and has been proposed as a host material to immobilize highly radioactive materials from dismantled weapons and nuclear waste from power stations [1]. In these applications zircon is exposed to alpha-irradiation. Computer simulations have started to be employed to simulate radiation damage in zircon [2], but the origin and microscopic mechanisms of the most important structural changes in zircon - unit cell expansion and large macroscopic swelling at higher doses, strong shear deformation of the crystalline lattice, and polymerization of SiOn units [3], remain unknown. Here, we perform the molecular dynamics simulation of highly energetic recoils in zircon. Basing on the simulation results, we propose the simple picture of the density change in the damaged region that consists of the depleted and densified matter. We find that the experimentally observed structural changes originate from the interaction of the damaged region with the surrounding crystalline lattice: the shear of the lattice around the damaged region causes shear deformation and expansion of the unit cells. The polymers of connected SiOn polyhedra are most commonly present in the densified shell at the periphery of the damaged region. [1] R C Ewing et al, J. Mater. Res. 10, 243 (1995); W J Weber et al, B E Burakov et al, in Scientific Basis for Nuclear Waste Management XIX, 25-32 and 33-40 (Plenum, New York, 1996); R C Ewing, et al in Crystalline Ceramics: Waste Forms for the Disposal of Weapons Plutonium, NATO Workshop Proceedings 65 (Academic Publishers, Dordrecht, The Netherlands, 1996). [2] B Park et al, Phys. Rev. B, 64, 174108 (1-16) (2001); J P Crocombette and D Ghaleb, J. Nucl. Mater., 295, 167 (2001); K Trachenko et al, J. Appl. Phys., 87, 7702 (2000); K Trachenko et al, J. Phys.: Cond. Matt., 13, 1947 (2001). [3] T Murakami et al, Am. Min., 76, 1510 (1991); H D Holland and D Gottfried, Acta Cryst. 8, 291 (1955).; W J Weber, J. Am

  8. Dynamical interaction of helium bubbles with cascade damage in Fe-9Cr ferritic alloy.

    SciTech Connect

    Ono, K.; Miyamoto, M.; Arakawa, K.; Birtcher, R. C.; Materials Science Division; Shimane Univ.; Osaka Univ.

    2008-12-01

    Dynamic interaction of helium bubble with cascade damage in Fe-9Cr ferritic alloy has been studied using in situ irradiation and electron microscopy. During the irradiation of the alloy by 400 keV Fe{sup +} ions at temperatures where no thermal motion takes place, induced displacement of small helium bubbles was observed: the bubbles underwent sporadic and instant displacement. The displacement was of the order of a few nanometers. The experimentally determined displacement probability of helium bubbles is consistent with the calculated probability of their dynamic interaction with sub-cascades introduced by the irradiation. Furthermore, during the irradiation of the alloy at higher temperatures, both retarded and accelerated Brownian type motions were observed. These results are discussed on the basis of dynamic interaction of helium bubbles with point defects that survive through high-energy self-ion irradiation.

  9. Helium Reionization Simulations. I. Modeling Quasars as Radiation Sources

    NASA Astrophysics Data System (ADS)

    La Plante, Paul; Trac, Hy

    2016-09-01

    We introduce a new project to understand helium reionization using fully coupled N-body, hydrodynamics, and radiative transfer simulations. This project aims to capture correctly the thermal history of the intergalactic medium as a result of reionization and make predictions about the Lyα forest and baryon temperature–density relation. The dominant sources of radiation for this transition are quasars, so modeling the source population accurately is very important for making reliable predictions. In this first paper, we present a new method for populating dark matter halos with quasars. Our set of quasar models includes two different light curves, a lightbulb (simple on/off) and symmetric exponential model, and luminosity-dependent quasar lifetimes. Our method self-consistently reproduces an input quasar luminosity function given a halo catalog from an N-body simulation, and propagates quasars through the merger history of halo hosts. After calibrating quasar clustering using measurements from the Baryon Oscillation Spectroscopic Survey, we find that the characteristic mass of quasar hosts is {M}h∼ 2.5× {10}12 {h}-1 {M}ȯ for the lightbulb model, and {M}h∼ 2.3× {10}12 {h}-1 {M}ȯ for the exponential model. In the latter model, the peak quasar luminosity for a given halo mass is larger than that in the former, typically by a factor of 1.5–2. The effective lifetime for quasars in the lightbulb model is 59 Myr, and in the exponential case, the effective time constant is about 15 Myr. We include semi-analytic calculations of helium reionization, and discuss how to include these quasars as sources of ionizing radiation for full hydrodynamics with radiative transfer simulations in order to study helium reionization.

  10. Repair of radiation damage in mammalian cells

    SciTech Connect

    Setlow, R.B.

    1981-01-01

    The responses, such as survival, mutation, and carcinogenesis, of mammalian cells and tissues to radiation are dependent not only on the magnitude of the damage to macromolecular structures - DNA, RNA, protein, and membranes - but on the rates of macromolecular syntheses of cells relative to the half-lives of the damages. Cells possess a number of mechanisms for repairing damage to DNA. If the repair systems are rapid and error free, cells can tolerate much larger doses than if repair is slow or error prone. It is important to understand the effects of radiation and the repair of radiation damage because there exist reasonable amounts of epidemiological data that permits the construction of dose-response curves for humans. The shapes of such curves or the magnitude of the response will depend on repair. Radiation damage is emphasized because: (a) radiation dosimetry, with all its uncertainties for populations, is excellent compared to chemical dosimetry; (b) a number of cancer-prone diseases are known in which there are defects in DNA repair and radiation results in more chromosomal damage in cells from such individuals than in cells from normal individuals; (c) in some cases, specific radiation products in DNA have been correlated with biological effects, and (d) many chemical effects seem to mimic radiation effects. A further reason for emphasizing damage to DNA is the wealth of experimental evidence indicating that damages to DNA can be initiating events in carcinogenesis.

  11. Radiation effects on microstructure and hardness of a titanium aluminide alloy irradiated by helium ions at room and elevated temperatures

    NASA Astrophysics Data System (ADS)

    Wei, Tao; Zhu, Hanliang; Ionescu, Mihail; Dayal, Pranesh; Davis, Joel; Carr, David; Harrison, Robert; Edwards, Lyndon

    2015-04-01

    A 45XD TiAl alloy possessing a lamellar microstructure was irradiated using 5 MeV helium ions to a fluence of 5 × 1021 ion m-2 (5000 appm) with a dose of about 1 dpa (displacements per atom). A uniform helium ion stopping damage region about 17 μm deep from the target surface was achieved by applying an energy degrading wheel. Radiation damage defects including helium-vacancy clusters and small helium bubbles were found in the microstructure of the samples irradiated at room temperature. With increasing irradiation temperature to 300 °C and 500 °C helium bubbles were clearly observed in both the α2 and γ phases of the irradiated microstructure. By means of nanoindentation significant irradiation hardening was measured. For the samples irradiated at room temperature the hardness increased from 5.6 GPa to 8.5 GPa and the irradiation-hardening effect reduced to approximately 8.0 GPa for the samples irradiated at 300 °C and 500 °C.

  12. Radiation damage in barium fluoride detector materials

    SciTech Connect

    Levey, P.W.; Kierstead, J.A.; Woody, C.L.

    1988-01-01

    To develop radiation hard detectors, particularly for high energy physics studies, radiation damage is being studied in BaF/sub 2/, both undoped and doped with La, Ce, Nd, Eu, Gd and Tm. Some dopants reduce radiation damage. In La doped BaF/sub 2/ they reduce the unwanted long lifetime luminescence which interferes with the short-lived fluorescence used to detect particles. Radiation induced coloring is being studied with facilities for making optical measurements before, during and after irradiation with /sup 60/C0 gamma rays. Doses of 10/sup 6/ rad, or less, create only ionization induced charge transfer effects since lattice atom displacement damage is negligible at these doses. All crystals studied exhibit color center formation, between approximately 200 and 800 nm, during irradiation and color center decay after irradiation. Thus only measurements made during irradiation show the total absorption present in a radiation field. Both undoped and La doped BaF/sub 2/ develop damage at minimum detectable levels in the UV---which is important for particle detectors. For particle detector applications these studies must be extended to high dose irradiations with particles energetic enough to cause lattice atom displacement damage. In principle, the reduction in damage provided by dopants could apply to other applications requiring radiation damage resistant materials.

  13. Gamma Radiation Damage in Silicon

    NASA Astrophysics Data System (ADS)

    Chang, Chensen

    A theory for interpreting carrier removal in terms of trap production has been derived from the carrier distribution function, which provides a relationship between the carrier removal rate and trap production rates due to the radiation damage. The carrier removal rate is a function of trap production as well as Fermi level position. Also, the carrier removal rate depends on many parameters, which are the density of states of the valance band as well as the conduction band, density of doping impurities, temperature, location of donor and acceptor energy levels and location of trap energy levels. P-type and n-type silicon Schottky diodes are irradiated by cobalt 60 gamma rays. The experimental results show that the carrier removal rate is dependent on the initial carrier concentration. Carrier concentrations are determined by room temperature C-V measurements while the trap production rates are determined by DLTS from measurements from 50 K to room temperature. A model presented by Williams, et al. for the carrier concentration vs. fluence, has been rederived from simple semiconductor carrier statistical mechanics. This model has then been extended to yield an expression for the initial carrier removal rate which depends on the production rate of each defect trap level in the band gap. We have tested these models thoroughly for the first time by measuring the trap production rates by DLTS, and then, using this information to calculate carrier removal rate and carrier concentration vs. fluence, we have verified that the results of the model can explain these same relationships obtained experimentally by C-V measurements. We believe that this is the first time that DLTS results have been linked directly to such simple and useful measurements as carrier removal rate and carrier concentration vs. fluence in a convincing manner. The success of this procedure also suggests that there are no "hidden" levels or traps which contribute to carrier removal rate but which do not

  14. Seismic Radiation from Material Damage During Explosions

    NASA Astrophysics Data System (ADS)

    Rodgers, A. J.; Ben-Zion, Y.

    2010-12-01

    Recent theoretical results on seismic representation from regions undergoing rapid material damage indicate that changes of elastic moduli can produce radiation that may be, in some cases, a significant portion of (or even larger than) the radiation from the standard moment source (Ben-Zion and Ampuero, 2009). The additional radiation is associated with a “damage-related source term” involving the product of the changes in the elasticity tensor and the total elastic strain tensor. The damage source term is non-zero in a certain volume where brittle damage occurs. The generated seismic motion can be computed, as for the classical moment source, by a convolution of the damage density in the affected volume with the spatial derivative of a Green’s functions for an earth model. Here we attempt to provide estimates of the amount and types (isotropic and deviatoric) of the damage related radiation for explosion scenarios. Using both analytic solutions and three-dimensional elastic finite difference calculations, we compute and compare the waves generated by the classical moment and damage related source terms in a full space. We assume a purely isotropic explosion of size corresponding to low-yield nuclear explosions, a granite (hard-rock) whole-space, and that the damage occurs instantaneously. Using a simple iterative approach, we adjust the strain ɛij where it exceeds the yield strength of the rock ɛij_c and reduce the local elastic moduli in proportion to the difference (ɛij - ɛij_c). The calculated adjustments to the strain and reduction of elastic moduli are used to estimate the additional moment contribution due to excess strain (ɛij - ɛij_c) and the associated damage source term in the yielding region. Finally, the seismic radiation from the brittle damage process is computed and compared to the radiation generated by the moment of the explosion source and the additional inelastic relaxation in the yielding region.

  15. DNA Damage Signals and Space Radiation Risk

    NASA Technical Reports Server (NTRS)

    Cucinotta, Francis A.

    2011-01-01

    Space radiation is comprised of high-energy and charge (HZE) nuclei and protons. The initial DNA damage from HZE nuclei is qualitatively different from X-rays or gamma rays due to the clustering of damage sites which increases their complexity. Clustering of DNA damage occurs on several scales. First there is clustering of single strand breaks (SSB), double strand breaks (DSB), and base damage within a few to several hundred base pairs (bp). A second form of damage clustering occurs on the scale of a few kbp where several DSB?s may be induced by single HZE nuclei. These forms of damage clusters do not occur at low to moderate doses of X-rays or gamma rays thus presenting new challenges to DNA repair systems. We review current knowledge of differences that occur in DNA repair pathways for different types of radiation and possible relationships to mutations, chromosomal aberrations and cancer risks.

  16. Detection of Lyman-beta and Helium Resonance Radiation in the Night Sky.

    PubMed

    Young, J M; Carruthers, G R; Holmes, J C; Johnson, C Y; Patterson, N P

    1968-05-31

    A rocket-borne photometer has detected far ultraviolet night glow radiations that are identified as Lyman-beta (HI 1026 angstroms), and the helium lines at 304 or 584 angstroms, or at both. At an altitude of 227 kilometers the measured intensity for Lyman-beta was about 10 rayleighs. The discrimination characteristics of the broad-band helium radiation filter give helium line intensities, at 227 kilometers, of 4.8 and 12 rayleighs, respectively, pending identification of the wavelength of the radiation as 304 or 584 angstroms. These ultraviolet radiations appear sufficient to maintain the night E and F(1) regions of the ionosphere. PMID:17768892

  17. Effect of 800 keV argon ions pre-damage on the helium blister formation of tungsten exposed to 60 keV helium ions

    NASA Astrophysics Data System (ADS)

    Chen, Zhe; Han, Wenjia; Yu, Jiangang; Zhu, Kaigui

    2016-04-01

    This study aims to investigate the effect of Ar8+ ions pre-damage on the following He2+ irradiation behavior of polycrystalline tungsten. We compared the irradiation resistance performance against 60 keV He2+ ions of undamaged tungsten samples with that of pre-damaged samples which were preliminarily exposed to 800 keV Ar8+ ions at a fluence of 4 × 1019 ions m-2. The experimental results indicate that the helium blistering of tungsten could be effectively relieved by the Ar8+ ions pre-damage, while the retention of helium around low energy desorption sites in the pre-damaged tungsten was larger than that of the undamaged samples. A strong orientation dependence of blistering had been observed, with the blister occurred preferentially on the surface of grains with normal direction close to <111>. The Ar8+ ions irradiation-induced damage altered the morphology of helium bubbles in tungsten exposed to the following He2+ irradiation significantly. The intensity of helium release peaks at relatively low temperatures (<600 K) was enhanced due to Ar8+ ions pre-damage.

  18. Hydrogen and helium excitation by EUV radiation for the production of white-light flares

    NASA Technical Reports Server (NTRS)

    Poland, A. I.; Milkey, R. W.; Thompson, W. T.

    1988-01-01

    Non-LTE radiative transfer calculations for hydrogen and helium in a simple model atmosphere are used to demonstrate that EUV radiation cannot be the main energy source for white-light flares. The opacities in the Lyman continuum and the helium I and II continua are found to be much larger than the enhanced opacity in the visible hydrogen continuum. It is shown that the EUV radiation is absorbed before it can have a significant effect on the visible light continuum.

  19. The use of ion accelerators and synchrotron radiation to study the interactions of helium with metals

    SciTech Connect

    Donnelly, S.E.; Gilles, J.M.; Lucas, A.A.; Rife, J.C.

    1981-04-01

    A technique is described which examines the properties of helium trapped in bubbles in implanted metals. Helium implanted materials are characterized using resonant elastic proton backscattering and Transmission Electron Microscopy. Spectroscopic measurements using synchrotron radiation in the far vacuum ultraviolet are then performed to examine the density sensitive optical absorption resulting from the 1S - 2P transition in the implanted helium. Experimental data for helium implanted aluminum thin films are presented which indicate atomic densities in small (50A diameter) bubbles of the order of 10/sup 23/ atoms cm/sup -3/.

  20. Nanofoams Response to Radiation Damage

    SciTech Connect

    Fu, Engang; Serrano De Caro, Magdalena; Wang, Yongqiang; Nastasi, Michael; Zepeda-Ruiz, Luis; Bringa, Eduardo M.; Baldwin, Jon K.; Caro, Jose A.

    2012-07-30

    Conclusions of this presentation are: (1) np-Au foams were successfully synthesized by de-alloying process; (2) np-Au foams remain porous structure after Ne ion irradiation to 1 dpa; (3) SFTs were observed in irradiated np-Au foams with highest and intermediate flux, while no SFTs were observed with lowest flux; (4) SFTs were observed in irradiated np-Au foams at RT, whereas no SFTs were observed at LNT irradiation; (5) The diffusivity of vacancies in Au at RT is high enough so that the vacancies have enough time to agglomerate and thus collapse. As a result, SFTs were formed; (6) The high flux created much more damage/time, vacancies don't have enough time to diffuse or recombine. As a result, SFTs were formed.

  1. Radiation damage in MINP cells

    NASA Astrophysics Data System (ADS)

    Minahan, J. A.; Green, M. J.

    Experiments have been carried out to examine the effects of exposure to various fluence levels of 1 MeV electrons on 0.2 ohm-cm MINP silicon solar cell characteristics. Fluence levels ranged from 10 to the 14th e/sq cm to 3 x 10 to the 15th e/sq cm. Minority carrier diffusion lengths, Lbase, were derived from short circuit current calculations that included corrections for surface shadowing, reflection and emitter contribution to the short circuit current. From Lbase and fluences, a damage coefficient for diffusion length was calculated (1.4 x 10 to the -9th/electron) and compared with results obtained for other cell designs and base resistivities.

  2. Elastic softening of zircon by radiation damage

    SciTech Connect

    Salje, Ekhard K. H.

    2006-09-25

    The bulk modulus and the shear modulus of zircon soften by ca. 50% when zircon is amorphized by radiation damage. A theoretical description of the experimental findings is presented which shows that the elastic response on a zircon ceramics with radiation damage follows Hashin-Shtrikman [J. Mech. Phys. Solids 11, 127 (1963)] behavior with very narrow bounds. The elastic response depends, in good approximation, on the square of the volume fraction f{sub a} of the amorphized regions. In a slightly coarser approximation one finds an almost linear interpolation of the bulk and the shear modulus between those of the crystalline state and those of the fully amorphous state.

  3. Radiation damage effects in polarized deuterated ammonia

    SciTech Connect

    P.M. McKee

    2003-07-01

    Solid polarized targets utilizing deuterated ammonia, {sup 15}ND{sub 3}, offer an attractive combination of high polarization, high dilution factor and high resistance to polarization losses from radiation damage. Jefferson Laboratory Experiment E93-026 used {sup 15}ND{sub 3} as a target material in a five-month form factor measurement, allowing a detailed study of it's performance. The dependence of the deuteron polarization on received dose by the ammonia and the effectiveness of annealing the material to recover performance lost to radiation damage will be discussed.

  4. Probing Radiation Damage at the Molecular Level

    NASA Astrophysics Data System (ADS)

    Mason, N. J.; Smialek, M. A.; Moore, S. A.; Folkard, M.; Hoffmann, S. V.

    2006-12-01

    Radiation damage of DNA and other cellular components has traditionally been attributed to ionisation via direct impact of high-energy quanta or by complex radical chemistry. However recent research has shown that strand breaks in DNA may be initiated by secondary electrons and is strongly dependent upon the target DNA base identity. Such research provides the fascinating perspective that it is possible that radiation damage may be described and understood at an individual molecular level introducing new possibilites for therapy and perhaps providing an insight into the origins of life.

  5. Expansion of Collisional Radiative Model for Helium line ratio spectroscopy

    NASA Astrophysics Data System (ADS)

    Cinquegrani, David; Cooper, Chris; Forest, Cary; Milhone, Jason; Munoz-Borges, Jorge; Schmitz, Oliver; Unterberg, Ezekial

    2015-11-01

    Helium line ratio spectroscopy is a powerful technique of active plasma edge spectroscopy. It enables reconstruction of plasma edge parameters like electron density and temperature by use of suitable Collisional Radiative Models (CRM). An established approach is successful at moderate plasma densities (~1018m-3 range) and temperature (30-300eV), taking recombination and charge exchange to be negligible. The goal of this work is to experimentally explore limitations of this approach to CRM. For basic validation the Madison Plasma Dynamo eXperiment (MPDX) will be used. MPDX offers a very uniform plasma and spherical symmetry at low temperature (5-20 eV) and low density (1016 -1017m-3) . Initial data from MPDX shows a deviation in CRM results when compared to Langmuir probe data. This discrepancy points to the importance of recombination effects. The validated model is applied to first time measurement of electron density and temperature in front of an ICRH antenna at the TEXTOR tokamak. These measurements are important to understand RF coupling and PMI physics at the antenna limiters. Work supported in part by start up funds of the Department of Engineering Physics at the UW - Madison, USA and NSF CAREER award PHY-1455210.

  6. Neutron dosimetry, damage calculations, and helium measurements for the HFIR-MFE-60J-1 and MFE-330J-1 spectral tailoring experiments

    SciTech Connect

    Greenwood, L.R.; Baldwin, C.A.; Oliver, B.M.

    1995-04-01

    The objective is to provide dosimetry and damage analysis for fusion materials irradiation experiments. Neutron fluence measurements and radiation damage calculations are reported for the joint US -Japanese MFE-60J-1 and MFE-330J-1 experiments in the hafnium-lined removable beryllium (RB{sup *}) position of the High Flux Isotope Reactor (HFIR) at Oak Ridge National Laboratory. These experiments were continuations of the ORR-6J and 7J irradiations performed in the Oak Ridge Research Reactor. The combination of irradiations was designed to tailor the neutron spectrum in order to achieve fusion reactor helium/dpa levels in stainless steel. These experiments produced maximum helium (appm)/dpa(displacement per atom) levels of 10.2 at 18.5 dpa for the ORR-6J and HFIR-MFE-60J-1 combination and 11.8 at 19.0 dpa for the ORR-7J and HFIR-MFE-330J-1 combination. A helium measurement in one JPCA sample was in good agreement with helium calculations.

  7. Radiation-Induced Helium Nanobubbles Enhance Ductility in Submicron-Sized Single-Crystalline Copper.

    PubMed

    Ding, Ming-Shuai; Du, Jun-Ping; Wan, Liang; Ogata, Shigenobu; Tian, Lin; Ma, Evan; Han, Wei-Zhong; Li, Ju; Shan, Zhi-Wei

    2016-07-13

    The workability and ductility of metals usually degrade with exposure to irradiation, hence the phrase "radiation damage". Here, we found that helium (He) radiation can actually enhance the room-temperature deformability of submicron-sized copper. In particular, Cu single crystals with diameter of 100-300 nm and containing numerous pressurized sub-10 nm He bubbles become stronger, more stable in plastic flow and ductile in tension, compared to fully dense samples of the same dimensions that tend to display plastic instability (strain bursts). The sub-10 nm He bubbles are seen to be dislocation sources as well as shearable obstacles, which promote dislocation storage and reduce dislocation mean free path, thus contributing to more homogeneous and stable plasticity. Failure happens abruptly only after significant bubble coalescence. The current findings can be explained in light of Weibull statistics of failure and the beneficial effects of bubbles on plasticity. These results shed light on plasticity and damage developments in metals and could open new avenues for making mechanically robust nano- and microstructures by ion beam processing and He bubble engineering. PMID:27249672

  8. Role of interfaces i nthe design of ultra-high strength, radiation damage tolerant nanocomposites

    SciTech Connect

    Misra, Amit; Wang, Yongqiang; Nastasi, Michael A; Baldwin, Jon K; Wei, Qiangmin; Li, Nan; Mara, Nathan; Zhang, Xinghang; Fu, Engang; Anderoglu, Osman; Li, Hongqi; Bhattacharyya, Dhriti

    2010-12-09

    The combination of high strength and high radiation damage tolerance in nanolaminate composites can be achieved when the individual layers in these composites are only a few nanometers thick and contain special interfaces that act both as obstacles to slip, as well as sinks for radiation-induced defects. The morphological and phase stabilities and strength and ductility of these nano-composites under ion irradiation are explored as a function of layer thickness, temperature and interface structure. Magnetron sputtered metallic multilayers such as Cu-Nb and V-Ag with a range of individual layer thickness from approximately 2 nm to 50 nm and the corresponding 1000 nm thick single layer films were implanted with helium ions at room temperature. Cross-sectional Transmission Electron Microscopy (TEM) was used to measure the distribution of helium bubbles and correlated with the helium concentration profile measured vis ion beam analysis techniques to obtain the helium concentration at which bubbles are detected in TEM. It was found that in multilayers the minimum helium concentration to form bubbles (approximately I nm in size) that are easily resolved in through-focus TEM imaging was several atomic %, orders of magnitude higher than that in single layer metal films. This observation is consistent with an increased solubility of helium at interfaces that is predicted by atomistic modeling of the atomic structures of fcc-bcc interfaces. At helium concentrations as high as 7 at.%, a uniform distribution of I nm diameter bubbles results in negligible irradiation hardening and loss of deformability in multi layers with layer thicknesses of a few nanometers. The control of atomic structures of interfaces to produce high helium solubility at interfaces is crucial in the design of nano-composite materials that are radiation damage tolerant. Reduced radiation damage also leads to a reduction in the irradiation hardening, particularly at layer thickness of approximately 5 run

  9. The Status of Radiation Damage Experiments

    SciTech Connect

    Strachan, Denis M.; Scheele, Randall D.; Icenhower, Jonathan P.; Kozelisky, Anne E.; Sell, Richard L.; Legore, Virginia L.; Schaef, Herbert T.; O'Hara, Matthew J.; Brown, Christopher F.; Buchmiller, William C.

    2001-11-20

    Experiments have been on-going for about two years to determine the effects that radiation damage have on the physical and chemical properties of candidate titanate ceramics for the immobilization of plutonium. We summarize the results of these experiments in this document.

  10. Undulator Radiation Damage Experience at LCLS

    SciTech Connect

    Nuhn, H. D.; Field, C.; Mao, S.; Levashov, Y.; Santana, M.; Welch, J. N.; Wolf, Z.

    2015-01-06

    The SLAC National Accelerator Laboratory has been running the Linac Coherent Light Source (LCLS), the first x-ray Free Electron Laser since 2009. Undulator magnet damage from radiation, produced by the electron beam traveling through the 133-m long straight vacuum tube, has been and is a concern. A damage measurement experiment has been performed in 2007 in order to obtain dose versus damage calibrations. Radiation reduction and detection devices have been integrated into the LCLS undulator system. The accumulated radiation dose rate was continuously monitored and recorded. In addition, undulator segments have been routinely removed from the beamline to be checked for magnetic (50 ppm, rms) and mechanic (about 0.25 µm, rms) changes. A reduction in strength of the undulator segments is being observed, at a level, which is now clearly above the noise. Recently, potential sources for the observed integrated radiation levels have been investigated. The paper discusses the results of these investigation as well as comparison between observed damage and measured dose accumulations and discusses, briefly, strategies for the new LCLS-II upgrade, which will be operating at more than 300 times larger beam rate.

  11. Acoustic emission sensor radiation damage threshold experiment

    SciTech Connect

    Beeson, K.M.; Pepper, C.E.

    1994-09-01

    Determination of the threshold for damage to acoustic emission sensors exposed to radiation is important in their application to leak detection in radioactive waste transport and storage. Proper response to system leaks is necessary to ensure the safe operation of these systems. A radiation impaired sensor could provide ``false negative or false positive`` indication of acoustic signals from leaks within the system. Research was carried out in the Radiochemical Technology Division at Oak Ridge National Laboratory to determine the beta/gamma radiation damage threshold for acoustic emission sensor systems. The individual system consisted of an acoustic sensor mounted with a two part epoxy onto a stainless steel waveguide. The systems were placed in an irradiation fixture and exposed to a Cobalt-60 source. After each irradiation, the sensors were recalibrated by Physical Acoustics Corporation. The results were compared to the initial calibrations performed prior to irradiation and a control group, not exposed to radiation, was used to validate the results. This experiment determines the radiation damage threshold of each acoustic sensor system and verifies its life expectancy, usefulness and reliability for many applications in radioactive environments.

  12. Gallium arsenide solar cell radiation damage study

    NASA Technical Reports Server (NTRS)

    Maurer, R. H.; Herbert, G. A.; Kinnison, J. D.; Meulenberg, A.

    1989-01-01

    A thorough analysis has been made of electron- and proton- damaged GaAs solar cells suitable for use in space. It is found that, although some electrical parametric data and spectral response data are quite similar, the type of damage due to the two types of radiation is different. An I-V analysis model shows that electrons damage the bulk of the cell and its currents relatively more, while protons damage the junction of the cell and its voltages more. It is suggested that multiple defects due to protons in a strong field region such as a p/n junction cause the greater degradation in cell voltage, whereas the individual point defects in the quasi-neutral minority-carrier-diffusion regions due to electrons cause the greater degradation in cell current and spectral response.

  13. Density Functional Theory Models for Radiation Damage

    NASA Astrophysics Data System (ADS)

    Dudarev, S. L.

    2013-07-01

    Density functional theory models developed over the past decade provide unique information about the structure of nanoscale defects produced by irradiation and about the nature of short-range interaction between radiation defects, clustering of defects, and their migration pathways. These ab initio models, involving no experimental input parameters, appear to be as quantitatively accurate and informative as the most advanced experimental techniques developed for the observation of radiation damage phenomena. Density functional theory models have effectively created a new paradigm for the scientific investigation and assessment of radiation damage effects, offering new insight into the origin of temperature- and dose-dependent response of materials to irradiation, a problem of pivotal significance for applications.

  14. Gravitational and radiative effects on the escape of helium from the moon

    NASA Technical Reports Server (NTRS)

    Hodges, R. R., Jr.

    1978-01-01

    On the moon, and probably on Mercury and other similar regolith-covered bodies with tenuous atmosphere, the dominant gas is He-4. It arises as the radiogenic product of the decay of uranium and thorium within any planet, but its major source appears to be the alpha particle flux of the solar wind. The moon intercepts solar wind helium at an average rate of 1.1 times 10 to the 24th atom/sec, and loses it at the same rate. Some helium may escape directly as the result of the process of solar wind soil bombardment which may release previously trapped helium at superthermal speeds. Atmospheric models have been calculated with the total helium influx as source. Subsequent comparison of model and measured helium concentrations indicates that the fraction of helium escaping via the atmosphere may range from 20% to 100% of the solar wind influx. Of the escaping atmosphere, most of the helium (about 93%) becomes trapped in earth orbit, while about 5% gets trapped in satellite orbits about the moon. Owing to a 6 month lifetime for helium in solar radiation, the satellite atoms form a lunar corona that exceeds the lunar atmosphere in total abundance by a factor of 4 to 5.

  15. Radiation damage in nanostructured metallic films

    NASA Astrophysics Data System (ADS)

    Yu, Kaiyuan

    High energy neutron and charged particle radiation cause microstructural and mechanical degradation in structural metals and alloys, such as phase segregation, void swelling, embrittlement and creep. Radiation induced damages typically limit nuclear materials to a lifetime of about 40 years. Next generation nuclear reactors require materials that can sustain over 60 - 80 years. Therefore it is of great significance to explore new materials with better radiation resistance, to design metals with favorable microstructures and to investigate their response to radiation. The goals of this thesis are to study the radiation responses of several nanostructured metallic thin film systems, including Ag/Ni multilayers, nanotwinned Ag and nanocrystalline Fe. Such systems obtain high volume fraction of boundaries, which are considered sinks to radiation induced defects. From the viewpoint of nanomechanics, it is of interest to investigate the plastic deformation mechanisms of nanostructured films, which typically show strong size dependence. By controlling the feature size (layer thickness, twin spacing and grain size), it is applicable to picture a deformation mechanism map which also provides prerequisite information for subsequent radiation hardening study. And from the viewpoint of radiation effects, it is of interest to explore the fundamentals of radiation response, to examine the microstructural and mechanical variations of irradiated nanometals and to enrich the design database. More importantly, with the assistance of in situ techniques, it is appealing to examine the defect generation, evolution, annihilation, absorption and interaction with internal interfaces (layer interfaces, twin boundaries and grain boundaries). Moreover, well-designed nanostructures can also verify the speculation that radiation induced defect density and hardening show clear size dependence. The focus of this thesis lies in the radiation response of Ag/Ni multilayers and nanotwinned Ag

  16. Nonuniform radiation damage in permanent magnet quadrupoles

    SciTech Connect

    Danly, C. R.; Merrill, F. E.; Barlow, D.; Mariam, F. G.

    2014-08-15

    We present data that indicate nonuniform magnetization loss due to radiation damage in neodymium-iron-boron Halbach-style permanent magnet quadrupoles. The proton radiography (pRad) facility at Los Alamos uses permanent-magnet quadrupoles for magnifying lenses, and a system recently commissioned at GSI-Darmsdadt uses permanent magnets for its primary lenses. Large fluences of spallation neutrons can be produced in close proximity to these magnets when the proton beam is, intentionally or unintentionally, directed into the tungsten beam collimators; imaging experiments at LANL’s pRad have shown image degradation with these magnetic lenses at proton beam doses lower than those expected to cause damage through radiation-induced reduction of the quadrupole strength alone. We have observed preferential degradation in portions of the permanent magnet quadrupole where the field intensity is highest, resulting in increased high-order multipole components.

  17. Nonuniform radiation damage in permanent magnet quadrupoles.

    PubMed

    Danly, C R; Merrill, F E; Barlow, D; Mariam, F G

    2014-08-01

    We present data that indicate nonuniform magnetization loss due to radiation damage in neodymium-iron-boron Halbach-style permanent magnet quadrupoles. The proton radiography (pRad) facility at Los Alamos uses permanent-magnet quadrupoles for magnifying lenses, and a system recently commissioned at GSI-Darmsdadt uses permanent magnets for its primary lenses. Large fluences of spallation neutrons can be produced in close proximity to these magnets when the proton beam is, intentionally or unintentionally, directed into the tungsten beam collimators; imaging experiments at LANL's pRad have shown image degradation with these magnetic lenses at proton beam doses lower than those expected to cause damage through radiation-induced reduction of the quadrupole strength alone. We have observed preferential degradation in portions of the permanent magnet quadrupole where the field intensity is highest, resulting in increased high-order multipole components. PMID:25173260

  18. Radiation damage in Luna 20 soil.

    NASA Technical Reports Server (NTRS)

    Phakey, P. P.; Price, P. B.

    1973-01-01

    As an extension of previous studies of radiation damage produced by heavy solar flare ions in lunar soils, high-voltage electron microscopy and electron diffraction procedures were used to rank a Luna 20 sample among the other soils returned by Soviet and American lunar missions. Micron-sized soil grains from the Luna 20 mission are the most lightly irradiated, in contrast to micron-sized grains from Luna 16 soil, which are the most heavily irradiated.

  19. Radiation-induced electrical breakdown of helium in fusion reactor superconducting magnet systems

    SciTech Connect

    Perkins, L.J.

    1983-12-02

    A comprehensive theoretical study has been performed on the reduction of the electrical breakdown potential of liquid and gaseous helium under neutron and gamma radiation. Extension of the conventional Townsend breakdown theory indicates that radiation fields at the superconducting magnets of a typical fusion reactor are potentially capable of significantly reducing currently established (i.e., unirradiated) helium breakdown voltages. Emphasis is given to the implications of these results including future deployment choices of magnet cryogenic methods (e.g., pool-boiling versus forced-flow), the possible impact on magnet shielding requirements and the analogous situation for radiation-induced electrical breakdown in fusion RF transmission systems.

  20. Comparison of Carbon and Main Ion Radiation Profiles in Matched Helium and Deuterium Plasmas in JET

    SciTech Connect

    Fenstermacher, M E; Lawson, K D; Porter, G D; Erents, S K; Ingesson, C; Mathews, G F; McCracken, G M; Philipps, V; Pitts, A; Stamp, M F

    2002-05-17

    This paper examines the radiation profiles and corresponding ionization source profiles of various carbon and main plasma ions in matched helium and deuterium L-mode plasmas in JET. Operation in helium should reduce chemical sputtering of carbon substantially compared with deuterium. The radiation intensities from C{sup 1+}, C{sup 2+} and C{sup 3+} in the helium plasmas showed reduction by factors of 8, 10 and 25 respectively along the inner SOL and divertor leg compared with the deuterium cases. However, the emission in the outer divertor leg was less than a factor of 2 lower in helium. Using photon efficiencies calculated by the UEDGE code for the spectrometer lines of sight, the calculated source rates of C{sup 1+} and C{sup 3+} along the inner SOL and inner divertor were reduced by a factors of 4 and 20 respectively in the helium plasmas. In the outer divertor leg the source rate of C{sup 3+} was reduced by a factor of 10 but the C{sup 1+} source rate did not change in helium. These measurements are consistent with a model that chemical sputtering of carbon dominates the source from the inner wall and inner divertor in deuterium L-mode plasmas while physical sputtering appears to dominate the source from the outer divertor.

  1. Radiation damage calculations for the LANSCE degrader

    SciTech Connect

    Ferguson, P.D.; Sommer, W.F.; Dudziak, D.J.; Wechsler, M.S.; Barnett, M.H.; Corzine, R.K.

    1998-09-01

    The A-6 water degrader at the Los Alamos Neutron Science Center (LANSCE) linear proton accelerator has an outer shell of Inconel 718. The degrader was irradiated by 800-MeV protons during 1988--1993 to an exposure of 5.3 ampere-hours (A h). As described in Ref. 1, material from the Inconel is currently being cut into specimens for microhardness, three-point bending, ball punch, microscopy, and corrosion tests. This paper is devoted to calculations of radiation damage, particularly displacement and He production, sustained by the degrader Inconel.

  2. Radiation damage of transition metal carbides

    SciTech Connect

    Dixon, G.

    1991-01-01

    In this grant period we have investigated electrical properties of transition metal carbides and radiation-induced defects produced by low-temperature electron irradiation in them. Special attention has been given to the composition VC[sub 0.88] in which the vacancies on the carbon sublattice of this fcc crystal order to produce a V[sub 8]C[sub 7] superlattice. The existence of this superlattice structure was found to make the crystal somewhat resistant to radiation damage at low doses and/or at ambient temperature. At larger doses significant changes in the resistivity are produced. Annealing effects were observed which we believe to be connected with the reconstitution of the superlattice structure.

  3. Radiation damage in cubic-stabilized zirconia

    SciTech Connect

    Costantini, Jean-Marc; Beuneu, Francois; Weber, William J

    2013-01-01

    Cubic yttria-stabilized zirconia (YSZ) can be used for nuclear applications as an inert matrix for actinide immobilization or transmutation. Indeed, the large amount of native oxygen vacancies leads to a high radiation tolerance of this material owing to defect recombination occurring in the atomic displacements cascades induced by fast neutron irradiation or ion implantations, as showed by Molecular dynamics (MD) simulations. Amorphization cannot be obtained in YSZ either by nuclear-collision or electronic-excitation damage, just like in urania. A kind of polygonization structure with slightly disoriented crystalline domains is obtained in both cases. In the first steps of damage, specific isolated point defects (like F+-type color centers) and point-defect clusters are produced by nuclear collisions with charged particles or neutrons. Further increase of damage leads to dislocation-loop formation, then to collapse of the dislocation network into a polygonization structure. For swift heavy ion irradiations, a similar polygonization structure is obtained above a threshold stopping power value of about 20-30 keV nm-1.

  4. Radiation damage in zircon and monazite

    SciTech Connect

    Meldrum, A.; Boatner, L.A.; Weber, W.J.; Ewing, R.C.

    1998-07-01

    Monazite and zircon respond differently to ion irradiation and to thermal and irradiation-enhanced annealing. The damage process (i.e., elastic interactions leading to amorphization) in radioactive minerals (metamictization) is basically the same as for the ion-beam-irradiated samples with the exception of the dose rate which is much lower in the case of natural samples. The crystalline-to-metamict transition in natural samples with different degrees of damage, from almost fully crystalline to completely metamict, is compared to the sequence of microstructures observed for ion-beam-irradiated monazite and zircon. The damage accumulation process, representing the competing effects of radiation-induced structural disorder and subsequent annealing mechanisms (irradiation-enhanced and thermal) occurs at much higher temperatures for zircon than for monazite. The amorphization dose, expressed as displacements per atom, is considerably higher in the natural samples, and the atomic-scale process leading to metamictization appears to develop differently. Ion-beam-induced amorphization data were used to calculate the {alpha}-decay-event dose required for amorphization in terms of a critical radionuclide concentration, i.e., the concentration above which a sample of a given age will become metamict at a specific temperature. This equation was applied to estimate the reliability of U-Pb ages, to provide a qualitative estimate of the thermal history of high-U natural zircons, and to predict whether actinide-bearing zircon or monazite nuclear waste forms will become amorphous (metamict) over long timescales.

  5. Correlation between thermoluminescence and radiation damage in bismuth germanate

    SciTech Connect

    Melcher, C.L.

    1985-02-01

    Thermoluminescence properties of bismuth germanate and their relationship to radiation damage characteristics have been investigated. Thermoluminescence and radiation damage in bismuth germanate display several similar properties including similar responses as a function of radiation dose, similar saturation levels, and similar decay times. Also a correlation was found between the thermoluminescence sensitivities and radiation damage sensitivities of four different crystals. The traps responsible for the radiation damage and those which store the thermoluminescence signal appear to be either closely related or actually the same traps. Four trapping centers can be seen in the thermoluminescence glow curves. The depth of the dominant trap is 1.1 eV. 10 references.

  6. Optical diagnostics with radiation trapping effect in low density and low temperature helium plasma

    NASA Astrophysics Data System (ADS)

    Lee, Wonwook; Park, Kyungdeuk; Kwon, Duck-Hee; Oh, Cha-Hwan

    2016-06-01

    Low density (ne < 1011 cm-3) and low temperature (Te < 10 eV) helium plasma was generated by hot filament discharge. Electron temperature and density of neutral helium plasma were measured by Langmuir probe and were determined by line intensity ratio method using optical emission spectroscopy with population modelings. Simple corona model and collisional-radiative (CR) model without consideration for radiation trapping effect are applied. In addition, CR model taking into account the radiation trapping effect (RTE) is adopted. The change of single line intensity ratio as a function of electron temperature and density were investigated when the RTE is included and excluded. The changes of multi line intensity ratios as a function of electron temperature were scanned for various radiative-excitation rate coefficients from the ground state and the helium gas pressures related with the RTE. Our CR modeling with RTE results in fairly better agreement of the spectroscopic diagnostics for the plasma temperature or density with the Langmuir probe measurements for various helium gas pressures than corona modeling and CR modeling without RTE.

  7. Energy Distribution of Electrons in Radiation Induced-Helium Plasmas. Ph.D. Thesis

    NASA Technical Reports Server (NTRS)

    Lo, R. H.

    1972-01-01

    Energy distribution of high energy electrons as they slow down and thermalize in a gaseous medium is studied. The energy distribution in the entire energy range from source energies down is studied analytically. A helium medium in which primary electrons are created by the passage of heavy-charged particles from nuclear reactions is emphasized. A radiation-induced plasma is of interest in a variety of applications, such as radiation pumped lasers and gaseous core nuclear reactors.

  8. Radiation Damage In Reactor Cavity Concrete

    SciTech Connect

    Field, Kevin G; Le Pape, Yann; Naus, Dan J; Remec, Igor; Busby, Jeremy T; Rosseel, Thomas M; Wall, Dr. James Joseph

    2015-01-01

    License renewal up to 60 years and the possibility of subsequent license renewal to 80 years has established a renewed focus on long-term aging of nuclear generating stations materials, and recently, on concrete. Large irreplaceable sections of most nuclear generating stations include concrete. The Expanded Materials Degradation Analysis (EMDA), jointly performed by the Department of Energy, the Nuclear Regulatory Commission and Industry, identified the urgent need to develop a consistent knowledge base on irradiation effects in concrete. Much of the historical mechanical performance data of irradiated concrete does not accurately reflect typical radiation conditions in NPPs or conditions out to 60 or 80 years of radiation exposure. To address these potential gaps in the knowledge base, The Electric Power Research Institute and Oak Ridge National Laboratory are working to disposition radiation damage as a degradation mechanism. This paper outlines the research program within this pathway including: (i) defining the upper bound of the neutron and gamma dose levels expected in the biological shield concrete for extended operation (80 years of operation and beyond), (ii) determining the effects of neutron and gamma irradiation as well as extended time at temperature on concrete, (iii) evaluating opportunities to irradiate prototypical concrete under accelerated neutron and gamma dose levels to establish a conservative bound and share data obtained from different flux, temperature, and fluence levels, (iv) evaluating opportunities to harvest and test irradiated concrete from international NPPs, (v) developing cooperative test programs to improve confidence in the results from the various concretes and research reactors, (vi) furthering the understanding of the effects of radiation on concrete (see companion paper) and (vii) establishing an international collaborative research and information exchange effort to leverage capabilities and knowledge.

  9. Surface damage in ultrafine and multimodal grained tungsten materials induced by low energy helium irradiation

    NASA Astrophysics Data System (ADS)

    El-Atwani, Osman; Efe, Mert; Heim, Bryan; Allain, Jean Paul

    2013-03-01

    Although tungsten is considered the best candidate as a plasma facing component (PFC) in the divertor region in the International Thermonuclear Experimental Reactor (ITER), severe morphology changes such as cavities, blisters, bubbles and nanostructure formation are expected. Increasing defect sinks in the tungsten microstructure is one of the possible solutions to mitigate the irradiation damage. In this work, helium irradiation at low energy (50 and 200 eV) and temperatures of 600 (threshold of vacancy migration) and 950 °C were performed on multimodal and ultrafine grained tungsten prepared by spark plasma sintering and severe plastic deformation (SPD), respectively. The multimodal samples consisted of small grains (300-700 nm size) juxtaposed to larger grains (1-3 μm size). Detachment of the small grains was observed in the multimodal grained tungsten irradiated at 600 °C and a fluence of 1 × 1022 m-2 due to grain boundary grooving. On the same sample but at 950 °C, detachment and nanostructuring of the small grains were observed together with recrystallization of the large grains. Irradiation of the SPD samples at 200 eV and 950 °C to a fluence of about 2 × 1022 m-2, resulted in nanostructuring of the ultrafine grained shear bands in the microstructure.

  10. Synergistic damage by UVA radiation and pollutants.

    PubMed

    Burke, K E; Wei, H

    2009-01-01

    Not only is skin cancer by far the most common human cancer but also the incidence of skin cancer has been increasing at an alarming rate in recent decades. Fortunately, most people now realize that sun exposure causes unattractive photoaging and skin cancer, so they do apply sunscreens conscientiously. However, until recently, most sunscreens did not adequately protect against ultraviolet A (UVA) radiation. Although UVA is indeed less erythrogenic and less carcinogenic than UVB, UVA directly causes photoaging and enhances UVB-induced skin cancer. Furthermore, recent research demonstrates that UVA combined with environmental pollutants (including cigarette smoke) significantly increases the risk of skin cancer. Similarly, previous research demonstrated another synergy between environmental pollutants and UV: When ozone exposure precedes UV exposure, there is enhancement of UV-induced depletion of protective vitamin E from the skin's stratum corneum. This article reviews experimental evidence that environmental pollutants (such as benzo[a]pyrene (BaP), a commonly used index of environmental pollution) are photosensitizers that generate reactive oxygen species (ROS) when exposed to UVA radiation. This in turn causes oxidative and genetic damage, leading to unattractive photodamage and carcinogenesis. PMID:19651790

  11. Chemistry of radiation damage to wire chambers

    SciTech Connect

    Wise, J.

    1992-08-01

    Proportional counters are used to study aspects of radiation damage to wire chambers (wire aging). Principles of low-pressure, rf plasma chemistry are used to predict the plasma chemistry in electron avalanches (1 atm, dc). (1) Aging is studied in CF{sub 4}/iC{sub 4}H{sub 10} gas mixtures. Wire deposits are analyzed by Auger electron spectroscopy. An apparent cathode aging process resulting in loss of gain rather than in a self-sustained current is observed in CF{sub 4}-rich gases. A four-part model considering plasma polymerization of the hydrocarbon, etching of wire deposits by CF{sub 4}, acceleration of deposition processes in strongly etching environments, and reactivity of the wire surface is developed to understand anode wire aging in CF{sub 4}/iC{sub 4}H{sub 10} gases. Practical guidelines suggested by the model are discussed. (2) Data are presented to suggest that trace amounts of Freons do not affect aging rates in either dimethyl ether or Ar/C{sub 2}H{sub 6}. Apparent loss of gain is explained by attachment of primary electrons to a continuously increasing concentration of Freon 11 (CCl{sub 3}F) in the counter gas. An increase in the concentration of Freon 11 in dimethyl ether is caused by a distillation process in the gas supply bottle and is a natural consequence of the unequal volatilities of the two compounds.

  12. Radiation and gas conduction heat transport across a helium dewer multilayer insulation system

    SciTech Connect

    Green, M.A.

    1995-02-01

    This report describes a method for calculating mixed heat transfer through the multilayer insulation used to insulated a 4K liquid helium cryostat. The method described permits one to estimate the insulation potential for a multilayer insulation system from first principles. The heat transfer regimes included are: radiation, conduction by free molecule gas conduction, and conduction through continuum gas conduction. Heat transfer in the transition region between the two gas conduction regimes is also included.

  13. Radiation damage calculations for the APT materials test program

    SciTech Connect

    Corzine, R.K.; Wechsler, M.S.; Dudziak, D.J.; Ferguson, P.D.; James, M.R.

    1999-09-01

    A materials irradiation was performed at the Los Alamos Neutron Science Center (LANSCE) in the fall of 1996 and spring of 1997 in support of the Accelerator Production of Tritium (APT) program. Testing of the irradiated materials is underway. In the proposed APT design, materials in the target and blanket are to be exposed to protons and neutrons over a wide range of energies. The irradiation and testing program was undertaken to enlarge the very limited direct knowledge presently available of the effects of medium-energy protons ({approximately}1 GeV) on the properties of engineering materials. APT candidate materials were placed in or near the LANSCE accelerator 800-MeV, 1-mA proton beam and received roughly the same proton current density in the center of the beam as would be the case for the APT facility. As a result, the proton fluences achieved in the irradiation were expected to approach the APT prototypic full-power-year values. To predict accurately the performance of materials in APT, radiation damage parameters for the materials experiment must be determined. By modeling the experiment, calculations for atomic displacement, helium and hydrogen cross sections and for proton and neutron fluences were done for representative samples in the 17A, 18A, and 18C areas. The LAHET code system (LCS) was used to model the irradiation program, LAHET 2.82 within LCS transports protons > 1 MeV, and neutrons >20 MeV. A modified version of MCNP for use in LCS, HMCNP 4A, was employed to tally neutrons of energies <20 MeV.

  14. Resonant two-photon absorption of extreme-ultraviolet free-electron-laser radiation in helium

    SciTech Connect

    Nagasono, Mitsuru; Suljoti, Edlira; Pietzsch, Annette; Hennies, Franz; Wellhoefer, Michael; Hoeft, Jon-Tobias; Martins, Michael; Wurth, Wilfried; Foehlisch, Alexander; Treusch, Rolf; Feldhaus, Josef; Schneider, Jochen R.

    2007-05-15

    We have investigated the nonlinear response of helium to intense extreme-ultraviolet radiation from the free-electron laser in Hamburg (FLASH). We observe a spectral feature between 24 and 26 eV electron kinetic energy in photoemission which shows a quadratic fluence dependence. The feature is explained as a result of subsequent processes involving a resonant two-photon absorption process into doubly excited levels of even parity (N=5 and 6), radiative decay to the doubly excited states in the vicinity of the He{sup +} (N=2) ionization threshold and finally the photoionization of the inner electron by the radiation of the next microbunches. This observation suggests that even-parity states, which have been elusive to be measured with the low pulse energy of synchrotron radiation sources, can be investigated with the intense radiation of FLASH. This also demonstrates a first step to bring nonlinear spectroscopy into the xuv and soft-x-ray regime.

  15. UV Radiation Damage and Bacterial DNA Repair Systems

    ERIC Educational Resources Information Center

    Zion, Michal; Guy, Daniel; Yarom, Ruth; Slesak, Michaela

    2006-01-01

    This paper reports on a simple hands-on laboratory procedure for high school students in studying both radiation damage and DNA repair systems in bacteria. The sensitivity to ultra-violet (UV) radiation of both "Escherichia coli" and "Serratia marcescens" is tested by radiating them for varying time periods. Two growth temperatures are used in…

  16. DNA damage and repair after high LET radiation

    NASA Astrophysics Data System (ADS)

    O'Neill, Peter; Cucinotta, Francis; Anderson, Jennifer

    Predictions from biophysical models of interactions of radiation tracks with cellular DNA indicate that clustered DNA damage sites, defined as two or more lesions formed within one or two helical turns of the DNA by passage of a single radiation track, are formed in mammalian cells. These complex DNA damage sites are regarded as a signature of ionizing radiation exposure particularly as the likelihood of clustered damage sites arising endogenously is low. For instance, it was predicted from biophysical modelling that 30-40% of low LET-induced double strand breaks (DSB), a form of clustered damage, are complex with the yield increasing to >90% for high LET radiation, consistent with the reduced reparability of DSB with increasing ionization density of the radiation. The question arises whether the increased biological effects such as mutagenesis, carcinogenesis and lethality is in part related to DNA damage complexity and/or spatial distribution of the damage sites, which may lead to small DNA fragments. With particle radiation it is also important to consider not only delta-rays which may cause clustered damaged sites and may be highly mutagenic but the non-random spatial distribution of DSB which may lead to deletions. In this overview I will concentrate on the molecular aspects of the variation of the complexity of DNA damage on radiation quality and the challenges this complexity presents the DNA damage repair pathways. I will draw on data from micro-irradiations which indicate that the repair of DSBs by non-homologous end joining is highly regulated with pathway choice and kinetics of repair dependent on the chemical complexity of the DSB. In summary the aim is to emphasis the link between the spatial distribution of energy deposition events related to the track, the molecular products formed and the consequence of damage complexity contributing to biological effects and to present some of the outstanding molecular challenges with particle radiation.

  17. Thermal conductivity measurements via time-domain thermoreflectance for the characterization of radiation induced damage

    DOE PAGESBeta

    Cheaito, Ramez; Gorham, Caroline S.; Carnegie Mellon Univ., Pittsburgh, PA; Misra, Amit; Hattar, Khalid; Hopkins, Patrick E.

    2015-05-01

    The progressive build up of displacement damage and fission products inside different systems and components of a nuclear reactor can lead to significant defect formation, degradation, and damage of the constituent materials. This structural modification can highly influence the thermal transport mechanisms and various mechanical properties of solids. In this paper we demonstrate the use of time-domain thermoreflectance (TDTR), a non-destructive method capable of measuring the thermal transport in material systems from nano to bulk scales, to study the effect of radiation damage and the subsequent changes in the thermal properties of materials. We use TDTR to show that displacementmore » damage from ion irradiation can significantly reduce the thermal conductivity of Optimized ZIRLO, a material used as fuel cladding in several current nuclear reactors. We find that the thermal conductivity of copper-niobium nanostructured multilayers does not change with helium ion irradiation doses of up to 1015 cm-2 and ion energy of 200 keV suggesting that these structures can be used and radiation tolerant materials in nuclear reactors. We compare the effect of ion doses and ion beam energies on the measured thermal conductivity of bulk silicon. Results demonstrate that TDTR thermal measurements can be used to quantify depth dependent damage.« less

  18. Thermal conductivity measurements via time-domain thermoreflectance for the characterization of radiation induced damage

    SciTech Connect

    Cheaito, Ramez; Gorham, Caroline S.; Misra, Amit; Hattar, Khalid; Hopkins, Patrick E.

    2015-05-01

    The progressive build up of displacement damage and fission products inside different systems and components of a nuclear reactor can lead to significant defect formation, degradation, and damage of the constituent materials. This structural modification can highly influence the thermal transport mechanisms and various mechanical properties of solids. In this paper we demonstrate the use of time-domain thermoreflectance (TDTR), a non-destructive method capable of measuring the thermal transport in material systems from nano to bulk scales, to study the effect of radiation damage and the subsequent changes in the thermal properties of materials. We use TDTR to show that displacement damage from ion irradiation can significantly reduce the thermal conductivity of Optimized ZIRLO, a material used as fuel cladding in several current nuclear reactors. We find that the thermal conductivity of copper-niobium nanostructured multilayers does not change with helium ion irradiation doses of up to 1015 cm-2 and ion energy of 200 keV suggesting that these structures can be used and radiation tolerant materials in nuclear reactors. We compare the effect of ion doses and ion beam energies on the measured thermal conductivity of bulk silicon. Results demonstrate that TDTR thermal measurements can be used to quantify depth dependent damage.

  19. Radiation Damage to Artemia Cysts:Effects of Water Vapor.

    PubMed

    Snipes, W C; Gordy, W

    1963-10-25

    Water vapor altered the form and greatly increased the rate of decay of the electron-spin resonance pattern of long-lived free radicals obtained upon gamma irradiation of Artemia salina cysts ( brine shrimp eggs). These results, combined with data on radiation survival, indicate that the water vapor protects the cysts from radiation damage, or heals the damage. They also indicate that water protects the cysts from the effect of oxygen by neutralizing the radiation-induced free radicals before they can interact with oxygen to produce irreversible damage. PMID:17748168

  20. Influence of Detector Radiation Damage on CR Mammography Quality Control.

    PubMed

    Moriwaki, Atsumi; Ishii, Mie; Terazono, Shiho; Arao, Keiko; Ishii, Rie; Sanada, Taizo; Yoshida, Akira

    2016-05-01

    Recently, radiation damage to the detector apparatus employed in computed radiography (CR) mammography has become problematic. The CR system and the imaging plate (IP) applied to quality control (QC) program were also used in clinical mammography in our hospital, and the IP to which radiation damage has occurred was used for approximately 5 years (approximately 13,000 exposures). We considered using previously acquired QC image data, which is stored in a server, to investigate the influence of radiation damage to an IP. The mammography unit employed in this study was a phase contrast mammography (PCM) Mermaid (KONICA MINOLTA) system. The QC image was made newly, and it was output in the film, and thereafter the optical density of the step-phantom image was measured. An input (digital value)-output (optical density) conversion curve was plotted using the obtained data. The digital values were then converted to optical density values using a reference optical density vs. digital value curve. When a high radiation dose was applied directly, radiation damage occurred at a position on the IP where no object was present. Daily QC for mammography is conducted using an American College of Radiology (ACR) accreditation phantom and acrylic disc, and an environmental background density measurement is performed as one of the management indexes. In this study, the radiation damage sustained by the acrylic disc was shown to differ from that of the background. Thus, it was revealed that QC results are influenced by radiation damage. PMID:27211088

  1. Enhanced annealing of GaAs solar cell radiation damage

    NASA Technical Reports Server (NTRS)

    Loo, R.; Knechtli, R. C.; Kamath, G. S.

    1981-01-01

    Solar cells are degraded by radiation damage in space. Investigations have been conducted concerning possibilities for annealing this radiation damage in GaAs solar cells, taking into account the conditions favoring such annealing. It has been found that continuous annealing as well as the combination of injection annealing with thermal annealing can lead to recovery from radiation damage under particularly favorable conditions in GaAs solar cells. The damage caused by both electrons and protons in GaAs solar cells can be substantially reduced by annealing at temperatures as low as 150 C, under appropriate conditions. This possibility makes the GaAs solar cells especially attractive for long space missions, or for missions in severe radiation environments. Attention is given to results concerning periodic thermal annealing, continuous annealing, and injection annealing combined with thermal annealing.

  2. Computer simulation of radiation damage in gallium arsenide

    NASA Technical Reports Server (NTRS)

    Stith, John J.; Davenport, James C.; Copeland, Randolph L.

    1989-01-01

    A version of the binary-collision simulation code MARLOWE was used to study the spatial characteristics of radiation damage in proton and electron irradiated gallium arsenide. Comparisons made with the experimental results proved to be encouraging.

  3. Radiation damage of gallium arsenide production cells

    NASA Technical Reports Server (NTRS)

    Mardesich, N.; Garlick, G. F. J.

    1987-01-01

    High-efficiency gallium arsenide cells, made by the liquid epitaxy method (LPE), have been irradiated with 1-MeV electrons up to fluences of 10 to the 16th e/sq cm. Measurements have been made of cell spectral response and dark and light-excited current-voltage characteristics and analyzed using computer-based models to determine underlying parameters such as damage coefficients. It is possible to use spectral response to sort out damage effects in the different cell component layers. Damage coefficients are similar to other reported in the literature for the emitter and buffer (base). However, there is also a damage effect in the window layer and possibly at the window emitter interface similar to that found for proton-irradiated liquid-phase epitaxy-grown cells. Depletion layer recombination is found to be less than theoretically expected at high fluence.

  4. Radiation damage effects on solid state detectors

    NASA Technical Reports Server (NTRS)

    Trainor, J. H.

    1972-01-01

    Totally depleted silicon diodes are discussed which are used as nuclear particle detectors in investigations of galactic and solar cosmic radiation and trapped radiation. A study of radiation and chemical effects on the diodes was conducted. Work on electron and proton irradiation of surface barrier detectors with thicknesses up to 1 mm was completed, and work on lithium-drifted silicon devices with thicknesses of several millimeters was begun.

  5. Effects Of Dose Rates On Radiation Damage In CMOS Parts

    NASA Technical Reports Server (NTRS)

    Goben, Charles A.; Coss, James R.; Price, William E.

    1990-01-01

    Report describes measurements of effects of ionizing-radiation dose rate on consequent damage to complementary metal oxide/semiconductor (CMOS) electronic devices. Depending on irradiation time and degree of annealing, survivability of devices in outer space, or after explosion of nuclear weapons, enhanced. Annealing involving recovery beyond pre-irradiation conditions (rebound) detrimental. Damage more severe at lower dose rates.

  6. Assessment of Radiation Damage to the Structural Material of EAST Tokamak

    NASA Astrophysics Data System (ADS)

    Chen, Yixue; Wu, Yican

    2005-08-01

    Radiation damage to structural material of fusion facilities is of high concern for safety. The superconducting tokamak EAST will conduct D-D plasma experiments with the neutron production of 1015 neutrons per second. To evaluate the material radiation damage a programme system has been devised with the Monte Carlo transport code MCNP-4C, the inventory code FISPACT99, a specific interface, and the fusion evaluated nuclear data library FENDL-2. The key nuclear responses, i.e. fast neutron flux, displacement per atom, and the helium and hydrogen production, are calculated for the structural material SS-316L of the first wall, and the vacuum vessel, using this programme. The results demonstrate that the radiation damage to the structural material is so little that it will not lead to any significant change of material properties according to the reference design. This indicates that there is a large potential space for EAST to test advanced operation regime from the viewpoint of structural material safety.

  7. Collisional-Radiative Model for Spectroscopic Diagnostic of Optically Thick Helium Plasma

    NASA Astrophysics Data System (ADS)

    Sawada, Keiji; Yamada, Yusuke; Miyachika, Takamasa; Ezumi, Naomichi; Iwamae, Atsushi; Goto, Motoshi

    We have included the effect of radiation trapping in a collisional-radiative model of neutral helium atoms developed by Goto [M. Goto, JQSRT 76, 331 (2003)], which is used to determine the electron temperature and density in plasmas from visible emission line intensities of atoms. In addition to the electron temperature and density, photo-excitation events from the ground state 11S to the 21P, 31P, and 41P states per second per one atom are treated as fitting parameters to reproduce the population density obtained by spectroscopic measurement. The model has been applied to an RF plasma at Shinshu University, Japan. The electron temperature and density and the contribution of radiation trapping to the population density of excited states are evaluated.

  8. COSMOSPEC: fast and detailed computation of the cosmological recombination radiation from hydrogen and helium

    NASA Astrophysics Data System (ADS)

    Chluba, Jens; Ali-Haïmoud, Yacine

    2016-03-01

    We present the first fast and detailed computation of the cosmological recombination radiation released during the hydrogen (redshift z ≃1300) and helium (z ≃2500 and 6000) recombination epochs, introducing the code COSMOSPEC. Our computations include important radiative transfer effects, 500-shell bound-bound and free-bound emission for all three species, the effects of electron scattering and free-free absorption as well as interspecies (He II⇒ He I⇒ H I) photon feedback. The latter effect modifies the shape and amplitude of the recombination radiation and COSMOSPEC improves significantly over previous treatments of it. Utilizing effective multilevel atom and conductance approaches, one calculation takes only ≃15 s on a standard laptop as opposed to days for previous computations. This is an important step towards detailed forecasts and feasibility studies considering the detection of the cosmological recombination lines and what one may hope to learn from the ≃6.1 photons emitted per hydrogen atom in the three recombination eras. We briefly illustrate some of the parameter dependences and discuss remaining uncertainties in particular related to collisional processes and the neutral helium atom model.

  9. Radiation damage of gallium arsenide production cells

    NASA Technical Reports Server (NTRS)

    Mardesich, N.; Joslin, D.; Garlick, J.; Lillington, D.; Gillanders, M.; Cavicchi, B.; Scott-Monck, J.; Kachare, R.; Anspaugh, B.

    1987-01-01

    High efficiency liquid phase epitaxy (LPE) gallium arsenide cells were irradiated with 1 Mev electrons up to fluences of 1 times 10 to the 16th power cm-2. Measurements of spectral response and dark and illuminated I-V data were made at each fluence and then, using computer codes, the experimental data was fitted to gallium arsenide cell models. In this way it was possible to determine the extent of the damage, and hence damage coefficients in both the emitter and base of the cell.

  10. Radiation damage in charge-coupled devices.

    PubMed

    Bassler, Niels

    2010-08-01

    Due to their high sensitivity and signal-to-noise ratio, charge-coupled devices (CCDs) have been the preferred optical photon detectors of astronomers for several decades. CCDs are flown in space as the main detection instrument on several well-known missions, such as the Hubble Space Telescope, XMM-Newton or the Cassini Probe. Also, CCDs are frequently used in satellite star trackers which provide attitude information to the satellite orientation system. However, one major drawback is their extreme vulnerability to radiation, which is readily abundant in space. Here, we shall give a brief overview of the radiation effects on CCDs, and mention ways how to mitigate the effects in other ways than merely increase shielding, such as cooling and annealing. As an example, we have investigated the radiation hardness of a particular CCD, the so-called CCD47-20 from Marconi Applied Technologies (now E2V), by exposing it to radiation fields representing the radiation environment found in a highly elliptic orbit crossing the Van-Allen radiation belts. Two engineering-grade CCDs were irradiated with proton beams and photons, and effects of increased bulk dark current, surface dark current and inversion threshold voltage shifts were observed and are quantified. PMID:20238121

  11. Electronic effects in high-energy radiation damage in tungsten

    DOE PAGESBeta

    Zarkadoula, Eva; Duffy, Dorothy M.; Nordlund, Kai; Seaton, M. A.; Todorov, I. T.; Weber, William J.; Trachenko, Kostya

    2015-01-01

    Even though the effects of the electronic excitations during high-energy radiation damage processes are not currently understood, it is shown that their role in the interaction of radiation with matter is important. We perform molecular dynamics simulations of high-energy collision cascades in bcc-tungsten using the coupled two-temperature molecular dynamics (2T-MD) model that incorporates both the effects of electronic stopping and electron–phonon interaction. We compare the combination of these effects on the induced damage with only the effect of electronic stopping, and conclude in several novel insights. In the 2T-MD model, the electron–phonon coupling results in less damage production in themore » molten region and in faster relaxation of the damage at short times. We show these two effects lead to a significantly smaller amount of the final damage at longer times.« less

  12. Electronic effects in high-energy radiation damage in tungsten

    SciTech Connect

    Zarkadoula, Eva; Duffy, Dorothy M.; Nordlund, Kai; Seaton, M. A.; Todorov, I. T.; Weber, William J.; Trachenko, Kostya

    2015-01-01

    Even though the effects of the electronic excitations during high-energy radiation damage processes are not currently understood, it is shown that their role in the interaction of radiation with matter is important. We perform molecular dynamics simulations of high-energy collision cascades in bcc-tungsten using the coupled two-temperature molecular dynamics (2T-MD) model that incorporates both the effects of electronic stopping and electron–phonon interaction. We compare the combination of these effects on the induced damage with only the effect of electronic stopping, and conclude in several novel insights. In the 2T-MD model, the electron–phonon coupling results in less damage production in the molten region and in faster relaxation of the damage at short times. We show these two effects lead to a significantly smaller amount of the final damage at longer times.

  13. Release of helium from irradiation damage in Fe?9Cr ferritic alloy

    NASA Astrophysics Data System (ADS)

    Ono, K.; Arakawa, K.; Shibasaki, H.; Kurata, H.; Nakamichi, I.; Yoshida, N.

    2004-08-01

    Thermal desorption of helium from Fe-9Cr and pure Fe which were irradiated with 5 keV He + ions at 85 or 473 K have been studied by thermal desorption spectrometry (TDS) at temperatures from room temperature to 1270 K and related microstructure changes by TEM. It is found that five TDS peaks appear in Fe-9Cr specimen and these peaks are well correlated with microstructure changes. From these results, the five peaks I Cr, II Cr, III Cr, IV Cr and V Cr may be attributed to release of trapped helium atoms by the break-up of vacancy-helium-self-interstitial atom complexes or very tiny loops, glide to the specimen surface or coalescence of interstitial type dislocation loops, shrinkage of dislocation loops, bubble migration to the specimen surface and α-γ phase transformation, respectively. Precipitation of Cr around the loops and bubbles in Fe-9Cr is revealed by STEM-EELS and this could shift the related TDS peaks to higher temperatures.

  14. Radiation damage to scintillator in the D0 luminosity monitor

    SciTech Connect

    Casey, Brendan; DeVaughan, Kayle; Enari, Yuji; Partridge, Richard; Yacoob, Sahal; /Northwestern U.

    2006-12-01

    We report the result of evaluating radiation damage to Bicron BC408 plastic scintillator used in the D0 Luminosity Monitor during Run IIa. The Luminosity Monitor provides pseudo-rapidity coverage over the range 2.7 < |{eta}| < 4.4, with the radiation dose in Run IIa estimated to be 0.5 MRad for the region closest to the beams. We find the light yield is degraded by 10-15% due to radiation damage by comparing new and old scintillator in four observables: (1) visual inspection, (2) optical transmittance, (3) response to the radioactive source of {sup 90}Sr and (4) light yield for cosmic rays.

  15. Radiation damage to nucleoprotein complexes in macromolecular crystallography

    PubMed Central

    Bury, Charles; Garman, Elspeth F.; Ginn, Helen Mary; Ravelli, Raimond B. G.; Carmichael, Ian; Kneale, Geoff; McGeehan, John E.

    2015-01-01

    Significant progress has been made in macromolecular crystallography over recent years in both the understanding and mitigation of X-ray induced radiation damage when collecting diffraction data from crystalline proteins. In contrast, despite the large field that is productively engaged in the study of radiation chemistry of nucleic acids, particularly of DNA, there are currently very few X-ray crystallographic studies on radiation damage mechanisms in nucleic acids. Quantitative comparison of damage to protein and DNA crystals separately is challenging, but many of the issues are circumvented by studying pre-formed biological nucleoprotein complexes where direct comparison of each component can be made under the same controlled conditions. Here a model protein–DNA complex C.Esp1396I is employed to investigate specific damage mechanisms for protein and DNA in a biologically relevant complex over a large dose range (2.07–44.63 MGy). In order to allow a quantitative analysis of radiation damage sites from a complex series of macromolecular diffraction data, a computational method has been developed that is generally applicable to the field. Typical specific damage was observed for both the protein on particular amino acids and for the DNA on, for example, the cleavage of base-sugar N1—C and sugar-phosphate C—O bonds. Strikingly the DNA component was determined to be far more resistant to specific damage than the protein for the investigated dose range. At low doses the protein was observed to be susceptible to radiation damage while the DNA was far more resistant, damage only being observed at significantly higher doses. PMID:25723923

  16. Space solar cells - High efficiency and radiation damage

    NASA Technical Reports Server (NTRS)

    Brandhorst, H. W., Jr.; Bernatowicz, D. T.

    1980-01-01

    The proceedings of the Third Solar Cell High Efficiency and Radiation Damage Meeting are outlined. The topics covered included high efficiency silicon solar cells, silicon solar cell radiation damage, GaAs solar cell performance, and 30 percent conversion devices. The study of radiation damage from a fundamental defect-centered basis is discussed and evaluated as a focus of future work. 18% AM0 efficiency and 0.7 V open-circuit voltages are designated as achievable goals for silicon solar cells, and the potential for 30% AM0 efficiencies from monolithic tandem cell designs without sunlight concentration is noted. In addition to its potential for 20% AM0 efficiencies, the GaAs cell offers the possibility of a radiation-insensitive power supply when operated at temperatures near 200 C.

  17. Radiation-induced DNA damage and chromatin structure

    NASA Technical Reports Server (NTRS)

    Rydberg, B.; Chatterjee, A. (Principal Investigator)

    2001-01-01

    DNA lesions induced by ionizing radiation in cells are clustered and not randomly distributed. For low linear energy transfer (LET) radiation this clustering occurs mainly on the small scales of DNA molecules and nucleosomes. For example, experimental evidence suggests that both strands of DNA on the nucleosomal surface can be damaged in single events and that this damage occurs with a 10-bp modulation because of protection by histones. For high LET radiation, clustering also occurs on a larger scale and depends on chromatin organization. A particularly significant clustering occurs when an ionizing particle traverses the 30 nm chromatin fiber with generation of heavily damaged DNA regions with an average size of about 2 kbp. On an even larger scale, high LET radiation can produce several DNA double-strand breaks in closer proximity than expected from randomness. It is suggested that this increases the probability of misrejoining of DNA ends and generation of lethal chromosome aberrations.

  18. Probing Radiation Damage in Plutonium Alloys with Multiple Measurement Techniques

    SciTech Connect

    McCall, S K; Fluss, M J; Chung, B W

    2010-04-21

    A material subjected to radiation damage will usually experience changes in its physical properties. Measuring these changes in the physical properties provides a basis to study radiation damage in a material which is important for a variety of real world applications from reactor materials to semiconducting devices. When investigating radiation damage, the relative sensitivity of any given property can vary considerably based on the concentration and type of damage present as well as external parameters such as the temperature and starting material composition. By measuring multiple physical properties, these differing sensitivities can be leveraged to provide greater insight into the different aspects of radiation damage accumulation, thereby providing a broader understanding of the mechanisms involved. In this report, self-damage from {alpha}-particle decay in Pu is investigated by measuring two different properties: magnetic susceptibility and resistivity. The results suggest that while the first annealing stage obeys second order chemical kinetics, the primary mechanism is not the recombination of vacancy-interstitial close pairs.

  19. Radiation-damaged tyrosinase molecules are inactive

    SciTech Connect

    Kempner, E.S.; Miller, J.H.

    1989-01-01

    Target analysis of radiation inactivation of mushroom tyrosinase yields different target sizes for diphenoloxidase and monophenoloxidase activities, which correspond to the subunits H and HL2 (or HL), respectively. After gel electrophoresis of irradiated samples, all diphenoloxidase activity is observed at the same position as seen in the original material. Radiolytic fragments contain no detectable activity, consistent with a fundamental assumption of target theory.

  20. Spectrum of complex DNA damages depends on the incident radiation

    NASA Astrophysics Data System (ADS)

    Hada, M.; Sutherland, B.

    Ionizing radiation induces clustered DNA damages in DNA-two or more abasic sites oxidized bases and strand breaks on opposite DNA strands within a few helical turns Clustered damages are considered to be difficult to repair and therefore potentially lethal and mutagenic damages Although induction of single strand breaks and isolated lesions has been studied extensively little is known of factors affecting induction of clusters other than double strand breaks DSB The aim of the present study was to determine whether the type of incident radiation could affect yield or spectra of specific clusters Genomic T7 DNA a simple 40 kbp linear blunt-ended molecule was irradiated in non-scavenging buffer conditions with Fe 970 MeV n Ti 980 MeV n C 293 MeV n Si 586 MeV n ions or protons 1 GeV n at the NASA Space Radiation Laboratory or with 100 kVp X-rays Irradiated DNA was treated with homogeneous Fpg or Nfo proteins or without enzyme treatment for DSB quantitation then electrophoresed in neutral agarose gels DSB Fpg-OxyPurine clusters and Nfo-Abasic clusters were quantified by number average length analysis The results show that the yields of all these complex damages depend on the incident radiation Although LETs are similar protons induced twice as many DSBs than did X-rays Further the spectrum of damage also depends on the radiation The yield damage Mbp Gy of all damages decreased with increasing linear energy transfer LET of the radiation The relative frequencies of DSBs to Abasic- and OxyBase clusters were higher

  1. Damage-tolerant nanotwinned metals with nanovoids under radiation environments.

    PubMed

    Chen, Y; Yu, K Y; Liu, Y; Shao, S; Wang, H; Kirk, M A; Wang, J; Zhang, X

    2015-01-01

    Material performance in extreme radiation environments is central to the design of future nuclear reactors. Radiation induces significant damage in the form of dislocation loops and voids in irradiated materials, and continuous radiation often leads to void growth and subsequent void swelling in metals with low stacking fault energy. Here we show that by using in situ heavy ion irradiation in a transmission electron microscope, pre-introduced nanovoids in nanotwinned Cu efficiently absorb radiation-induced defects accompanied by gradual elimination of nanovoids, enhancing radiation tolerance of Cu. In situ studies and atomistic simulations reveal that such remarkable self-healing capability stems from high density of coherent and incoherent twin boundaries that rapidly capture and transport point defects and dislocation loops to nanovoids, which act as storage bins for interstitial loops. This study describes a counterintuitive yet significant concept: deliberate introduction of nanovoids in conjunction with nanotwins enables unprecedented damage tolerance in metallic materials. PMID:25906997

  2. Damage-tolerant nanotwinned metals with nanovoids under radiation environments

    PubMed Central

    Chen, Y.; Yu, K Y.; Liu, Y.; Shao, S.; Wang, H.; Kirk, M. A.; Wang, J.; Zhang, X.

    2015-01-01

    Material performance in extreme radiation environments is central to the design of future nuclear reactors. Radiation induces significant damage in the form of dislocation loops and voids in irradiated materials, and continuous radiation often leads to void growth and subsequent void swelling in metals with low stacking fault energy. Here we show that by using in situ heavy ion irradiation in a transmission electron microscope, pre-introduced nanovoids in nanotwinned Cu efficiently absorb radiation-induced defects accompanied by gradual elimination of nanovoids, enhancing radiation tolerance of Cu. In situ studies and atomistic simulations reveal that such remarkable self-healing capability stems from high density of coherent and incoherent twin boundaries that rapidly capture and transport point defects and dislocation loops to nanovoids, which act as storage bins for interstitial loops. This study describes a counterintuitive yet significant concept: deliberate introduction of nanovoids in conjunction with nanotwins enables unprecedented damage tolerance in metallic materials. PMID:25906997

  3. Damage-tolerant nanotwinned metals with nanovoids under radiation environments

    DOE PAGESBeta

    Chen, Y.; Yu, K. Y.; Liu, Y.; Shao, S.; Wang, H.; Kirk, M. A.; Wang, J.; Zhang, X.

    2015-04-24

    Material performance in extreme radiation environments is central to the design of future nuclear reactors. Radiation induces significant damage in the form of dislocation loops and voids in irradiated materials, and continuous radiation often leads to void growth and subsequent void swelling in metals with low stacking fault energy. Here we show that by using in situ heavy ion irradiation in a transmission electron microscope, pre-introduced nanovoids in nanotwinned Cu efficiently absorb radiation-induced defects accompanied by gradual elimination of nanovoids, enhancing radiation tolerance of Cu. In situ studies and atomistic simulations reveal that such remarkable self-healing capability stems from highmore » density of coherent and incoherent twin boundaries that rapidly capture and transport point defects and dislocation loops to nanovoids, which act as storage bins for interstitial loops. This study describes a counterintuitive yet significant concept: deliberate introduction of nanovoids in conjunction with nanotwins enables unprecedented damage tolerance in metallic materials.« less

  4. Damage-tolerant nanotwinned metals with nanovoids under radiation environments

    NASA Astrophysics Data System (ADS)

    Chen, Y.; Yu, K. Y.; Liu, Y.; Shao, S.; Wang, H.; Kirk, M. A.; Wang, J.; Zhang, X.

    2015-04-01

    Material performance in extreme radiation environments is central to the design of future nuclear reactors. Radiation induces significant damage in the form of dislocation loops and voids in irradiated materials, and continuous radiation often leads to void growth and subsequent void swelling in metals with low stacking fault energy. Here we show that by using in situ heavy ion irradiation in a transmission electron microscope, pre-introduced nanovoids in nanotwinned Cu efficiently absorb radiation-induced defects accompanied by gradual elimination of nanovoids, enhancing radiation tolerance of Cu. In situ studies and atomistic simulations reveal that such remarkable self-healing capability stems from high density of coherent and incoherent twin boundaries that rapidly capture and transport point defects and dislocation loops to nanovoids, which act as storage bins for interstitial loops. This study describes a counterintuitive yet significant concept: deliberate introduction of nanovoids in conjunction with nanotwins enables unprecedented damage tolerance in metallic materials.

  5. Minimizing radiation damage in nonlinear optical crystals

    DOEpatents

    Cooke, D.W.; Bennett, B.L.; Cockroft, N.J.

    1998-09-08

    Methods are disclosed for minimizing laser induced damage to nonlinear crystals, such as KTP crystals, involving various means for electrically grounding the crystals in order to diffuse electrical discharges within the crystals caused by the incident laser beam. In certain embodiments, electrically conductive material is deposited onto or into surfaces of the nonlinear crystals and the electrically conductive surfaces are connected to an electrical ground. To minimize electrical discharges on crystal surfaces that are not covered by the grounded electrically conductive material, a vacuum may be created around the nonlinear crystal. 5 figs.

  6. Minimizing radiation damage in nonlinear optical crystals

    DOEpatents

    Cooke, D. Wayne; Bennett, Bryan L.; Cockroft, Nigel J.

    1998-01-01

    Methods are disclosed for minimizing laser induced damage to nonlinear crystals, such as KTP crystals, involving various means for electrically grounding the crystals in order to diffuse electrical discharges within the crystals caused by the incident laser beam. In certain embodiments, electrically conductive material is deposited onto or into surfaces of the nonlinear crystals and the electrically conductive surfaces are connected to an electrical ground. To minimize electrical discharges on crystal surfaces that are not covered by the grounded electrically conductive material, a vacuum may be created around the nonlinear crystal.

  7. DNA damage in cells exhibiting radiation-induced genomic instability

    DOE PAGESBeta

    Keszenman, Deborah J.; Kolodiuk, Lucia; Baulch, Janet E.

    2015-02-22

    Cells exhibiting radiation induced genomic instability exhibit varied spectra of genetic and chromosomal aberrations. Even so, oxidative stress remains a common theme in the initiation and/or perpetuation of this phenomenon. Isolated oxidatively modified bases, abasic sites, DNA single strand breaks and clustered DNA damage are induced in normal mammalian cultured cells and tissues due to endogenous reactive oxygen species generated during normal cellular metabolism in an aerobic environment. While sparse DNA damage may be easily repaired, clustered DNA damage may lead to persistent cytotoxic or mutagenic events that can lead to genomic instability. In this study, we tested the hypothesismore » that DNA damage signatures characterised by altered levels of endogenous, potentially mutagenic, types of DNA damage and chromosomal breakage are related to radiation-induced genomic instability and persistent oxidative stress phenotypes observed in the chromosomally unstable progeny of irradiated cells. The measurement of oxypurine, oxypyrimidine and abasic site endogenous DNA damage showed differences in non-double-strand breaks (DSB) clusters among the three of the four unstable clones evaluated as compared to genomically stable clones and the parental cell line. These three unstable clones also had increased levels of DSB clusters. The results of this study demonstrate that each unstable cell line has a unique spectrum of persistent damage and lead us to speculate that alterations in DNA damage signaling and repair may be related to the perpetuation of genomic instability.« less

  8. DNA damage in cells exhibiting radiation-induced genomic instability

    SciTech Connect

    Keszenman, Deborah J.; Kolodiuk, Lucia; Baulch, Janet E.

    2015-02-22

    Cells exhibiting radiation induced genomic instability exhibit varied spectra of genetic and chromosomal aberrations. Even so, oxidative stress remains a common theme in the initiation and/or perpetuation of this phenomenon. Isolated oxidatively modified bases, abasic sites, DNA single strand breaks and clustered DNA damage are induced in normal mammalian cultured cells and tissues due to endogenous reactive oxygen species generated during normal cellular metabolism in an aerobic environment. While sparse DNA damage may be easily repaired, clustered DNA damage may lead to persistent cytotoxic or mutagenic events that can lead to genomic instability. In this study, we tested the hypothesis that DNA damage signatures characterised by altered levels of endogenous, potentially mutagenic, types of DNA damage and chromosomal breakage are related to radiation-induced genomic instability and persistent oxidative stress phenotypes observed in the chromosomally unstable progeny of irradiated cells. The measurement of oxypurine, oxypyrimidine and abasic site endogenous DNA damage showed differences in non-double-strand breaks (DSB) clusters among the three of the four unstable clones evaluated as compared to genomically stable clones and the parental cell line. These three unstable clones also had increased levels of DSB clusters. The results of this study demonstrate that each unstable cell line has a unique spectrum of persistent damage and lead us to speculate that alterations in DNA damage signaling and repair may be related to the perpetuation of genomic instability.

  9. Experimental study of the effects of helium-neon laser radiation on repair of injured tendon

    NASA Astrophysics Data System (ADS)

    Xu, Yong-Qing; Li, Zhu-Yi; Weng, Long-Jiang; An, Mei; Li, Kai-Yun; Chen, Shao-Rong; Wang, Jian-Xin; Lu, Yu

    1993-03-01

    Despite extensive research into the biology of tendon healing, predictably restoring normal function to a digit after a flexor tendon laceration remains one of the most difficult problems facing the hand surgeon. The challenge of simultaneously achieving tendon healing while minimizing the peritendinous scar formation, which limits tendon gliding, has captured the attention of investigators for many years. It has been said that low-power density helium-neon laser radiation had effects on anti-inflammation, detumescence, progressive wound healing, and reducing intestinal adhesions. This experimental study aims at whether helium-neon laser can reduce injured tendon adhesions and improve functional recovery of the injured tendon. Fifty white Leghorn hens were used. Ten were randomly assigned as a normal control group, the other forty were used in the operation. After anesthetizing them with Amytal, a half of the profundus tendons of the second and third foretoes on both sides of the feet were cut. Postoperatively, the hens moved freely in the cages. One side of the toes operated on were randomly chosen as a treatment group, the other side served as an untreated control group. The injured tendon toes in the treatment group were irradiated for twenty minutes daily with a fiber light needle of helium-neon laser therapeutic apparatus (wavelength, 6328 angstroms) at a constant power density of 12.74 mW/cm2, the first exposure taking place 24 hours after the operation. The longest course of treatment was 3 weeks. The control group was not irradiated. At 3 days, 1, 2, 3, and 5 weeks after surgery, 8 hens were sacrificed and their tendons were examined. The experimental results: (1) active, passive flexion and tendon gliding functional recovery were significantly better in the treatment group (p < 0.01); (2) width and thickness of the tendon at the cut site were significantly smaller in the treatment group (p < 0.01); (3) degrees of tendon adhesions were significantly lighter

  10. Opportunities for nutritional amelioration of radiation-induced cellular damage.

    PubMed

    Turner, Nancy D; Braby, Leslie A; Ford, John; Lupton, Joanne R

    2002-10-01

    The closed environment and limited evasive capabilities inherent in space flight cause astronauts to be exposed to many potential harmful agents (chemical contaminants in the environment and cosmic radiation exposure). Current power systems used to achieve space flight are prohibitively expensive for supporting the weight requirements to fully shield astronauts from cosmic radiation. Therefore, radiation poses a major, currently unresolvable risk for astronauts, especially for long-duration space flights. The major detrimental radiation effects that are of primary concern for long-duration space flights are damage to the lens of the eye, damage to the immune system, damage to the central nervous system, and cancer. In addition to the direct damage to biological molecules in cells, radiation exposure induces oxidative damage. Many natural antioxidants, whether consumed before or after radiation exposure, are able to confer some level of radioprotection. In addition to achieving beneficial effects from long-known antioxidants such as vitamins E and C and folic acid, some protection is conferred by several recently discovered antioxidant molecules, such as flavonoids, epigallocatechin, and other polyphenols. Somewhat counterintuitive is the protection provided by diets containing elevated levels of omega-3 polyunsaturated fatty acids, considering they are thought to be prone to peroxidation. Even with the information we have at our disposal, it will be difficult to predict the types of dietary modifications that can best reduce the risk of radiation exposure to astronauts, those living on Earth, or those enduring diagnostic or therapeutic radiation exposure. Much more work must be done in humans, whether on Earth or, preferably, in space, before we are able to make concrete recommendations. PMID:12361786

  11. Opportunities for nutritional amelioration of radiation-induced cellular damage

    NASA Technical Reports Server (NTRS)

    Turner, Nancy D.; Braby, Leslie A.; Ford, John; Lupton, Joanne R.

    2002-01-01

    The closed environment and limited evasive capabilities inherent in space flight cause astronauts to be exposed to many potential harmful agents (chemical contaminants in the environment and cosmic radiation exposure). Current power systems used to achieve space flight are prohibitively expensive for supporting the weight requirements to fully shield astronauts from cosmic radiation. Therefore, radiation poses a major, currently unresolvable risk for astronauts, especially for long-duration space flights. The major detrimental radiation effects that are of primary concern for long-duration space flights are damage to the lens of the eye, damage to the immune system, damage to the central nervous system, and cancer. In addition to the direct damage to biological molecules in cells, radiation exposure induces oxidative damage. Many natural antioxidants, whether consumed before or after radiation exposure, are able to confer some level of radioprotection. In addition to achieving beneficial effects from long-known antioxidants such as vitamins E and C and folic acid, some protection is conferred by several recently discovered antioxidant molecules, such as flavonoids, epigallocatechin, and other polyphenols. Somewhat counterintuitive is the protection provided by diets containing elevated levels of omega-3 polyunsaturated fatty acids, considering they are thought to be prone to peroxidation. Even with the information we have at our disposal, it will be difficult to predict the types of dietary modifications that can best reduce the risk of radiation exposure to astronauts, those living on Earth, or those enduring diagnostic or therapeutic radiation exposure. Much more work must be done in humans, whether on Earth or, preferably, in space, before we are able to make concrete recommendations.

  12. High-energy radiation damage in zirconia: Modeling results

    NASA Astrophysics Data System (ADS)

    Zarkadoula, E.; Devanathan, R.; Weber, W. J.; Seaton, M. A.; Todorov, I. T.; Nordlund, K.; Dove, M. T.; Trachenko, K.

    2014-02-01

    Zirconia is viewed as a material of exceptional resistance to amorphization by radiation damage, and consequently proposed as a candidate to immobilize nuclear waste and serve as an inert nuclear fuel matrix. Here, we perform molecular dynamics simulations of radiation damage in zirconia in the range of 0.1-0.5 MeV energies with account of electronic energy losses. We find that the lack of amorphizability co-exists with a large number of point defects and their clusters. These, importantly, are largely isolated from each other and therefore represent a dilute damage that does not result in the loss of long-range structural coherence and amorphization. We document the nature of these defects in detail, including their sizes, distribution, and morphology, and discuss practical implications of using zirconia in intense radiation environments.

  13. High-energy radiation damage in zirconia: modeling results

    SciTech Connect

    Zarkadoula, Evangelia; Devanathan, Ram; Weber, William J; Seaton, M; Todorov, I T; Nordlund, Kai; Dove, Martin T; Trachenko, Kostya

    2014-01-01

    Zirconia is viewed as a material of exceptional resistance to amorphization by radiation damage, and consequently proposed as a candidate to immobilize nuclear waste and serve as an inert nuclear fuel matrix. Here, we perform molecular dynamics simulations of radiation damage in zirconia in the range of 0.1-0.5 MeV energies with account of electronic energy losses. We nd that the lack of amorphizability co-exists with a large number of point defects and their clusters. These, importantly, are largely isolated from each other and therefore represent a dilute damage that does not result in the loss of long-range structural coherence and amorphization. We document the nature of these defects in detail, including their sizes, distribution and morphology, and discuss practical implications of using zirconia in intense radiation environments.

  14. High-energy radiation damage in zirconia: modeling results

    SciTech Connect

    Zarkadoula, Eva; Devanathan, Ram; Weber, William J.; Seaton, Michael; Todorov, Ilian; Nordlund, Kai; Dove, Martin T.; Trachenko, Kostya

    2014-02-28

    Zirconia has been viewed as a material of exceptional resistance to amorphization by radiation damage, and was consequently proposed as a candidate to immobilize nuclear waste and serve as a nuclear fuel matrix. Here, we perform molecular dynamics simulations of radiation damage in zirconia in the range of 0.1-0.5 MeV energies with the account of electronic energy losses. We find that the lack of amorphizability co-exists with a large number of point defects and their clusters. These, importantly, are largely disjoint from each other and therefore represent a dilute damage that does not result in the loss of long-range structural coherence and amorphization. We document the nature of these defects in detail, including their sizes, distribution and morphology, and discuss practical implications of using zirconia in intense radiation environments.

  15. High-energy radiation damage in zirconia: Modeling results

    SciTech Connect

    Zarkadoula, E.; Devanathan, R.; Weber, W. J.; Seaton, M. A.; Todorov, I. T.; Nordlund, K.; Dove, M. T.; Trachenko, K.

    2014-02-28

    Zirconia is viewed as a material of exceptional resistance to amorphization by radiation damage, and consequently proposed as a candidate to immobilize nuclear waste and serve as an inert nuclear fuel matrix. Here, we perform molecular dynamics simulations of radiation damage in zirconia in the range of 0.1–0.5 MeV energies with account of electronic energy losses. We find that the lack of amorphizability co-exists with a large number of point defects and their clusters. These, importantly, are largely isolated from each other and therefore represent a dilute damage that does not result in the loss of long-range structural coherence and amorphization. We document the nature of these defects in detail, including their sizes, distribution, and morphology, and discuss practical implications of using zirconia in intense radiation environments.

  16. Studying Radiation Damage in Structural Materials by Using Ion Accelerators

    NASA Astrophysics Data System (ADS)

    Hosemann, Peter

    2011-02-01

    Radiation damage in structural materials is of major concern and a limiting factor for a wide range of engineering and scientific applications, including nuclear power production, medical applications, or components for scientific radiation sources. The usefulness of these applications is largely limited by the damage a material can sustain in the extreme environments of radiation, temperature, stress, and fatigue, over long periods of time. Although a wide range of materials has been extensively studied in nuclear reactors and neutron spallation sources since the beginning of the nuclear age, ion beam irradiations using particle accelerators are a more cost-effective alternative to study radiation damage in materials in a rather short period of time, allowing researchers to gain fundamental insights into the damage processes and to estimate the property changes due to irradiation. However, the comparison of results gained from ion beam irradiation, large-scale neutron irradiation, and a variety of experimental setups is not straightforward, and several effects have to be taken into account. It is the intention of this article to introduce the reader to the basic phenomena taking place and to point out the differences between classic reactor irradiations and ion irradiations. It will also provide an assessment of how accelerator-based ion beam irradiation is used today to gain insight into the damage in structural materials for large-scale engineering applications.

  17. Raman study of radiation-damaged zircon under hydrostatic compression

    NASA Astrophysics Data System (ADS)

    Nasdala, Lutz; Miletich, Ronald; Ruschel, Katja; Váczi, Tamás

    2008-12-01

    Pressure-induced changes of Raman band parameters of four natural, gem-quality zircon samples with different degrees of self-irradiation damage, and synthetic ZrSiO4 without radiation damage, have been studied under hydrostatic compression in a diamond anvil cell up to ~10 GPa. Radiation-damaged zircon shows similar up-shifts of internal SiO4 stretching modes at elevated pressures as non-damaged ZrSiO4. Only minor changes of band-widths were observed in all cases. This makes it possible to estimate the degree of radiation damage from the width of the ν3(SiO4) band of zircon inclusions in situ, almost independent from potential “fossilized pressures” or compressive strain acting on the inclusions. An application is the non-destructive analysis of gemstones such as corundum or spinel: broadened Raman bands are a reliable indicator of self-irradiation damage in zircon inclusions, whose presence allows one to exclude artificial color enhancement by high-temperature treatment of the specimen.

  18. ANL/WSU radiation damage studies

    SciTech Connect

    Jankowski, D.; Lopiano, D.; Proudfoot, J.; Underwood, D.; Miles, L.; Neidiger, J.; Tripard, G.

    1993-12-31

    We report preliminary results for the radiation hardness of (polystryrene) plastic scintillator stacks using a spectrum of energy hardened neutrons from a MARK-III TRIGA reactor. The total dose ranged from 100 KRad to 3MRad. The corresponding fluence was 3.8 {times} 10{sup 13} to 3.8 {times} 10{sup 14} (n/cm/cm) with the gamma contribution on the order 2--3% (of fluence). The measurements used Li-6, Li-7 Thermo-luminescence dosimeters. Radiochromic/GaF- Chromic film, and activated foils simultaneously allowing an inter-comparison of these various methods of dosimetry.

  19. Proton radiation damage in optical filter glass

    NASA Technical Reports Server (NTRS)

    Grillot, Patrick N.; Rosenberg, William J.

    1989-01-01

    Samples of Schott BG-39 and Hoya CM-500 blue-green filter glass were subjected to proton radiation to determine their acceptability for spaceflight. Initial testing done with 2.7 MeV protons showed negligible change in optical transmittance with doses as high as 5.2 x 10 to the 14th protons per sq cm. Irradiation with protons of energy up to 63 MeV caused a significant reduction in transmittance in the Schott samples at doses of 5.3 x 10 to the 12th protons per sq cm, while negligible change occurred in the Hoya samples.

  20. Multiscale approach to the physics of radiation damage with ions

    SciTech Connect

    Surdutovich, Eugene; Solov'yov, Andrey V.

    2013-04-19

    We review a multiscale approach to the physics of ion-beam cancer therapy, an approach suggested in order to understand the interplay of a large number of phenomena involved in radiation damage scenario occurring on a range of temporal, spatial, and energy scales. We briefly overview its history and present the current stage of its development. The differences of the multiscale approach from other methods of understanding and assessment of radiation damage are discussed as well as its relationship to other branches of physics, chemistry and biology.

  1. Assessing the Effects of Radiation Damage on Ni-base Alloys for the Prometheus Space Reactor System

    SciTech Connect

    T. Angeliu

    2006-01-19

    Ni-base alloys were considered for the Prometheus space reactor pressure vessel with operational parameters of {approx}900 K for 15 years and fluences up to 160 x 10{sup 20} n/cm{sup 2} (E > 0.1 MeV). This paper reviews the effects of irradiation on the behavior of Ni-base alloys and shows that radiation-induced swelling and creep are minor considerations compared to significant embrittlement with neutron ,exposure. While the mechanism responsible for radiation-induced embrittlement is not fully understood, it is likely a combination of helium embrittlement and solute segregation that can be highly dependent on the alloy composition and exposure conditions. Transmutation calculations show that detrimental helium levels would be expected at the end of life for the inner safety rod vessel (thimble) and possibly the outer pressure vessel, primarily from high energy (E > 1 MeV) n,{alpha} reactions with {sup 58}Ni. Helium from {sup 10}B is significant only for the outer vessel due to the proximity of the outer vessel to the Be0 control elements. Recommendations for further assessments of the material behavior and methods to minimize the effects of radiation damage through alloy design are provided.

  2. Assessing the Effects of Radiation Damage on Ni-base Alloys for the Prometheus Space Reactor System

    SciTech Connect

    T Angeliu; J Ward; J Witter

    2006-04-04

    Ni-base alloys were considered for the Prometheus space reactor pressure vessel with operational parameters of {approx}900 K for 15 years and fluences up to 160 x 10{sup 20} n/cm{sup 2} (E > 0.1 MeV). This paper reviews the effects of irradiation on the behavior of Ni-base alloys and shows that radiation-induced swelling and creep are minor considerations compared to significant embrittlement with neutron exposure. While the mechanism responsible for radiation-induced embrittlement is not fully understood, it is likely a combination of helium embrittlement and solute segregation that can be highly dependent on the alloy composition and exposure conditions. Transmutation calculations show that detrimental helium levels would be expected at the end of life for the inner safety rod vessel (thimble) and possibly the outer pressure vessel, primarily from high energy (E > 1 MeV) n,{alpha} reactions with {sup 58}Ni. Helium from {sup 10}B is significant only for the outer vessel due to the proximity of the outer vessel to the BeO control elements. Recommendations for further assessments of the material behavior and methods to minimize the effects of radiation damage through alloy design are provided.

  3. Computer simulation radiation damages in condensed matters

    NASA Astrophysics Data System (ADS)

    Kupchishin, A. I.; Kupchishin, A. A.; Voronova, N. A.; Kirdyashkin, V. I.; Gyngazov, V. A.

    2016-02-01

    As part of the cascade-probability method were calculated the energy spectra of primary knocked-out atoms and the concentration of radiation-induced defects in a number of metals irradiated by electrons. As follows from the formulas, the number of Frenkel pairs at a given depth depends on three variables having certain physical meaning: firstly, Cd (Ea h) is proportional to the average energy of the considered depth of the PKA (if it is higher, than the greater number of atoms it will displace); secondly is inversely proportional to the path length λ2 for the formation of the PKA (if λ1 is higher than is the smaller the probability of interaction) and thirdly is inversely proportional to Ed. In this case calculations are in satisfactory agreement with the experimental data (for example, copper and aluminum).

  4. Gallium Arsenide solar cell radiation damage experiment

    NASA Technical Reports Server (NTRS)

    Maurer, R. H.; Kinnison, J. D.; Herbert, G. A.; Meulenberg, A.

    1991-01-01

    Gallium arsenide (GaAs) solar cells for space applications from three different manufactures were irradiated with 10 MeV protons or 1 MeV electrons. The electrical performance of the cells was measured at several fluence levels and compared. Silicon cells were included for reference and comparison. All the GaAs cell types performed similarly throughout the testing and showed a 36 to 56 percent power areal density advantage over the silicon cells. Thinner (8-mil versus 12-mil) GaAs cells provide a significant weight reduction. The use of germanium (Ge) substrates to improve mechanical integrity can be implemented with little impact on end of life performance in a radiation environment.

  5. RNA protects a nucleoprotein complex against radiation damage.

    PubMed

    Bury, Charles S; McGeehan, John E; Antson, Alfred A; Carmichael, Ian; Gerstel, Markus; Shevtsov, Mikhail B; Garman, Elspeth F

    2016-05-01

    Radiation damage during macromolecular X-ray crystallographic data collection is still the main impediment for many macromolecular structure determinations. Even when an eventual model results from the crystallographic pipeline, the manifestations of radiation-induced structural and conformation changes, the so-called specific damage, within crystalline macromolecules can lead to false interpretations of biological mechanisms. Although this has been well characterized within protein crystals, far less is known about specific damage effects within the larger class of nucleoprotein complexes. Here, a methodology has been developed whereby per-atom density changes could be quantified with increasing dose over a wide (1.3-25.0 MGy) range and at higher resolution (1.98 Å) than the previous systematic specific damage study on a protein-DNA complex. Specific damage manifestations were determined within the large trp RNA-binding attenuation protein (TRAP) bound to a single-stranded RNA that forms a belt around the protein. Over a large dose range, the RNA was found to be far less susceptible to radiation-induced chemical changes than the protein. The availability of two TRAP molecules in the asymmetric unit, of which only one contained bound RNA, allowed a controlled investigation into the exact role of RNA binding in protein specific damage susceptibility. The 11-fold symmetry within each TRAP ring permitted statistically significant analysis of the Glu and Asp damage patterns, with RNA binding unexpectedly being observed to protect these otherwise highly sensitive residues within the 11 RNA-binding pockets distributed around the outside of the protein molecule. Additionally, the method enabled a quantification of the reduction in radiation-induced Lys and Phe disordering upon RNA binding directly from the electron density. PMID:27139628

  6. RNA protects a nucleoprotein complex against radiation damage

    PubMed Central

    Bury, Charles S.; McGeehan, John E.; Antson, Alfred A.; Carmichael, Ian; Gerstel, Markus; Shevtsov, Mikhail B.; Garman, Elspeth F.

    2016-01-01

    Radiation damage during macromolecular X-ray crystallographic data collection is still the main impediment for many macromolecular structure determinations. Even when an eventual model results from the crystallographic pipeline, the manifestations of radiation-induced structural and conformation changes, the so-called specific damage, within crystalline macromolecules can lead to false interpretations of biological mechanisms. Although this has been well characterized within protein crystals, far less is known about specific damage effects within the larger class of nucleoprotein complexes. Here, a methodology has been developed whereby per-atom density changes could be quantified with increasing dose over a wide (1.3–25.0 MGy) range and at higher resolution (1.98 Å) than the previous systematic specific damage study on a protein–DNA complex. Specific damage manifestations were determined within the large trp RNA-binding attenuation protein (TRAP) bound to a single-stranded RNA that forms a belt around the protein. Over a large dose range, the RNA was found to be far less susceptible to radiation-induced chemical changes than the protein. The availability of two TRAP molecules in the asymmetric unit, of which only one contained bound RNA, allowed a controlled investigation into the exact role of RNA binding in protein specific damage susceptibility. The 11-fold symmetry within each TRAP ring permitted statistically significant analysis of the Glu and Asp damage patterns, with RNA binding unexpectedly being observed to protect these otherwise highly sensitive residues within the 11 RNA-binding pockets distributed around the outside of the protein molecule. Additionally, the method enabled a quantification of the reduction in radiation-induced Lys and Phe disordering upon RNA binding directly from the electron density. PMID:27139628

  7. Radiation damage studies of silicon photomultipliers

    NASA Astrophysics Data System (ADS)

    Bohn, P.; Clough, A.; Hazen, E.; Heering, A.; Rohlf, J.; Freeman, J.; Los, S.; Cascio, E.; Kuleshov, S.; Musienko, Y.; Piemonte, C.

    2009-01-01

    We report on the measurement of the radiation hardness of silicon photomultipliers (SiPMs) manufactured by Fondazione Bruno Kessler in Italy (1 and 6.2 mm2), Center of Perspective Technology and Apparatus in Russia (1 and 4.4 mm2), and Hamamatsu Corporation in Japan (1 mm2). The SiPMs were irradiated using a beam of 212 MeV protons at Massachusetts General Hospital, receiving fluences of up to 3×1010 protons per cm2 with the SiPMs at operating voltage. Leakage currents were read continuously during the irradiation. The delivery of the protons was paused periodically to record scope traces in response to calibrated light pulses to monitor the gains, photon detection efficiencies, and dark counts of the SiPMs. The leakage current and dark noise are found to increase with fluence. The leakage current is found to be proportional to the mean square deviation of the noise distribution, indicating the dark counts are due to increased random individual pixel activation, while SiPMs remain fully functional as photon detectors. The SiPMs are found to anneal at room temperature with a reduction in the leakage current by a factor of 2 in about 100 days.

  8. Radiation damage aspects of the chernobyl accident

    NASA Astrophysics Data System (ADS)

    Parmentier, N.; Nenot, J. C.

    During the night of 25 to 26 April 1986, the most severe nuclear accident occurred at the Chernobyl power station, about 150km north of Kiev, in the Ukraine. It resulted in the irradiation of 237 workers at dose levels justifying medical care. The most severe cases (115) were hospitalized in Moscow, with 20 patients with doses higher than 6 Gy. In most cases, the treatment was classical, based on transfusion of red cells and platelets, and heavy supportive therapy. For 19 patients with severe aplasia, transplantations of bone marrow (13) or foetal liver (6) were decided. Of these patients only one survived, which justifies the statement from U.S.S.R. physicians: after an accident the indications of grafting are limited and its risks may not justify its use. Most of the complications were related to radiation burns which involved 56 victims and resulted in fatal outcomes in at least 19 patients. The population was evacuated from a 30 km zone around the site; based on direct measurements and calculations, the collective dose was evaluated at 1.6 × 10 4 man Sv, with an individual average lower than 250 mSv. The European part of U.S.S.R. with 75 million persons is supposed to have received a collective dose likely to increase the natural mortality by less than 0.1%. The numbers with cancer in the Northern Hemisphere might increase by 0.004% over the next 50 years.

  9. Effect of Rosiglitazone on Radiation Damage in Bone Marrow Hemopoiesis

    NASA Astrophysics Data System (ADS)

    Benkő, Klára; Pintye, Éva; Szabó, Boglárka; Géresi, Krisztina; Megyeri, Attila; Benkő, Ilona

    2008-12-01

    To study radiobiological effects and drugs, which can modify radiation injury, has an importance if we would like to avoid harmful effects of radiation due to emergency situations or treat patients with malignant diseases by radiotherapy. During the long treatment schedules patients may be treated by not only anticancer but many other drugs because of accompanying diseases. These drugs may also modify radiobiological effects. Rosiglitazone pre-treatment proved to be myeloprotective and accelerated recovery of 5-fluorouracil-damaged bone marrow in our previous experiments. Our new studies are designed to evaluate whether rosiglitazone has similar beneficial effects in radiation-damaged hemopoiesis. Bone marrow damage was precipitated by total body irradiation (TBI) using single increasing doses (2-10 Gy) of γ—irradiation in groups of mice. Lethality was well correlated with damage in hemopoiesis measured by cellularity of bone marrow (LD50 values were 4.8 and 5.3 gray respectively). Rosiglitazone, an insulin-sensitizing drug, had no significant effect on bone marrow cellularity. Insulin resistance associated with obesity or diabetes mellitus type 2 is intensively growing among cancer patients requiring some kind of radiotherapy. Therefore it is important to know whether drugs used for their therapy can modify radiation effects.

  10. Neutron dosimetry and radiation damage calculations for HFBR

    SciTech Connect

    Greenwood, L.R.; Ratner, R.T.

    1998-03-01

    Neutron dosimetry measurements have been conducted for various positions of the High Flux Beam Reactor (HFBR) at Brookhaven National Laboratory (BNL) in order to measure the neutron flux and energy spectra. Neutron dosimetry results and radiation damage calculations are presented for positions V10, V14, and V15.

  11. On the Use of SRIM for Computing Radiation Damage Exposure

    SciTech Connect

    Stoller, Roger E.; Toloczko, Mychailo B.; Was, Gary S.; Certain, Alicia G.; Dwaraknath, S.; Garner, Frank A.

    2013-09-01

    The SRIM (formerly TRIM) Monte Carlo simulation code is widely used to compute a number of parameters relevant to ion beam implantation and ion beam processing of materials. It also has the capability to compute a common radiation damage exposure unit known as atomic displacements per atom (dpa). Since dpa is a standard measure of primary radiation damage production, most researchers who employ ion beams as a tool for inducing radiation damage in materials use SRIM to determine the dpa associated with their irradiations. The use of SRIM for this purpose has been evaluated and comparisons have been made with an internationally-recognized standard definition of dpa, as well as more detailed atomistic simulations of atomic displacement cascades. Differences between the standard and SRIM-based dpa are discussed and recommendations for future usage of SRIM in radiation damage studies are made. In particular, it is recommended that when direct comparisons between ion and neutron data are intended, the Kinchin-Pease option of SRIM should be selected.

  12. Effect of Rosiglitazone on Radiation Damage in Bone Marrow Hemopoiesis

    SciTech Connect

    Benko', Klara; Pintye, Eva; Szabo, Boglarka; Geresi, Krisztina; Megyeri, Attila; Benko, Ilona

    2008-12-08

    To study radiobiological effects and drugs, which can modify radiation injury, has an importance if we would like to avoid harmful effects of radiation due to emergency situations or treat patients with malignant diseases by radiotherapy. During the long treatment schedules patients may be treated by not only anticancer but many other drugs because of accompanying diseases. These drugs may also modify radiobiological effects. Rosiglitazone pre-treatment proved to be myeloprotective and accelerated recovery of 5-fluorouracil-damaged bone marrow in our previous experiments. Our new studies are designed to evaluate whether rosiglitazone has similar beneficial effects in radiation-damaged hemopoiesis. Bone marrow damage was precipitated by total body irradiation (TBI) using single increasing doses (2-10 Gy) of {gamma}--irradiation in groups of mice. Lethality was well correlated with damage in hemopoiesis measured by cellularity of bone marrow (LD{sub 50} values were 4.8 and 5.3 gray respectively). Rosiglitazone, an insulin-sensitizing drug, had no significant effect on bone marrow cellularity. Insulin resistance associated with obesity or diabetes mellitus type 2 is intensively growing among cancer patients requiring some kind of radiotherapy. Therefore it is important to know whether drugs used for their therapy can modify radiation effects.

  13. On the use of SRIM for computing radiation damage exposure

    NASA Astrophysics Data System (ADS)

    Stoller, R. E.; Toloczko, M. B.; Was, G. S.; Certain, A. G.; Dwaraknath, S.; Garner, F. A.

    2013-09-01

    The SRIM (formerly TRIM) Monte Carlo simulation code is widely used to compute a number of parameters relevant to ion beam implantation and ion beam processing of materials. It also has the capability to compute a common radiation damage exposure unit known as atomic displacements per atom (dpa). Since dpa is a standard measure of primary radiation damage production, most researchers who employ ion beams as a tool for inducing radiation damage in materials use SRIM to determine the dpa associated with their irradiations. The use of SRIM for this purpose has been evaluated and comparisons have been made with an internationally-recognized standard definition of dpa, as well as more detailed atomistic simulations of atomic displacement cascades. Differences between the standard and SRIM-based dpa are discussed and recommendations for future usage of SRIM in radiation damage studies are made. In particular, it is recommended that when direct comparisons between ion and neutron data are intended, the Kinchin-Pease option of SRIM should be selected.

  14. Radiation damage effects in candidate titanates for Pu disposition: Pyrochlore

    NASA Astrophysics Data System (ADS)

    Strachan, D. M.; Scheele, R. D.; Buck, E. C.; Icenhower, J. P.; Kozelisky, A. E.; Sell, R. L.; Elovich, R. J.; Buchmiller, W. C.

    2005-10-01

    Laboratory experiments on titanate ceramics were performed to verify whether certain assumptions are valid regarding the swelling, chemical durability, and microcracking that might occur as 239Pu decays. Titanate ceramics are the material of choice for the immobilization of surplus weapons-grade Pu. The short-lived isotope 238Pu, was incorporated into the ceramic formulation to accelerate the effects of radiation-induced damage. We report on the effects of this damage on the density (volumetric swelling <6%), crystal structure of pyrochlore-bearing specimens (amorphous after about 2 × 1018 α/g), and dissolution (no change from the fully crystalline specimen). Even though the specimens became amorphous during the tests, there was no evidence for microcracking in the photomicrographs from the scanning electron microscope. Thus, although pyrochlore is susceptible to radiation-induced damage, the material remains chemically and physically viable as a material for immobilizing surplus weapons-grade Pu.

  15. Proton-induced radiation damage in germanium detectors

    NASA Technical Reports Server (NTRS)

    Brueckner, J.; Koerfer, M.; Waenke, H.; Schroeder, A. N. F.; Filges, D.; Dragovitsch, P.; Englert, P. A. J.; Starr, R.; Trombka, J. I.

    1991-01-01

    High-purity germanium (HPGe) detectors will be used in future space missions for gamma-ray measurements and will be subject to interactions with energetic particles. To simulate this process, several large-volume n-type HPGe detectors were incrementally exposed to a particle fluence of up to 10 to the 8th protons/sq cm (proton energy: 1.5 GeV) at different operating temperatures (90 to 120 K) to induce radiation damage. Basic scientific and engineering data on detector performance were collected. During the incremental irradiation, the peak shape produced by the detectors showed a significant change from a Gaussian shape to a broad complex structure. After the irradiation, all detectors were thoroughly characterized by measuring many parameters. To remove the accumulated radiation damage, the detectors were stepwise-annealed at temperatures below 110 C, while kept in their specially designed cryostats. This study shows that n-type HPGe detectors can be used in charged-particle environments as high-energy resolution devices until a certain level of radiation damage is accumulated and that the damage can be removed at moderate annealing temperatures and the detector returned to operating condition.

  16. Proton-induced radiation damage in germanium detectors

    SciTech Connect

    Bruckner, J.; Korfer, M.; Wanke, H. , Mainz ); Schroeder, A.N.F. ); Figes, D.; Dragovitsch, P. ); Englert, P.A.J. ); Starr, R.; Trombka, J.I. . Goddard Space Flight Center); Taylor, I. ); Drake, D.M.; Shunk, E.R. )

    1991-04-01

    High-purity germanium (HPGe) detectors will be used in future space missions for gamma-ray measurements and will be subject to interactions with energetic particles. To simulate this process several large-volume n-type HPGe detectors were incrementally exposed to a particle fluence of up to 10{sub 8} protons cm{sup {minus}2} (proton energy: 1.5 GeV) at different operating temperatures (90 to 120 K) to induce radiation damage. Basic scientific as well as engineering data on detector performance were collected. During the incremental irradiation, the peak shape produced by the detectors showed a significant change from a Gaussian shape to a broad complex structure. After the irradiation all detectors were thoroughly characterized by measuring many parameters. To remove the accumulated radiation damage the detectors were stepwise annealed at temperatures T {le} 110{degrees}C while staying specially designed cryostats. This paper shows that n-type HPGe detectors can be used in charged particles environments as high-energy resolution devices until a certain level of radiation damage is accumulated and that the damage can be removed at moderate annealing temperatures and the detector returned to operating condition.

  17. GUI to Facilitate Research on Biological Damage from Radiation

    NASA Technical Reports Server (NTRS)

    Cucinotta, Frances A.; Ponomarev, Artem Lvovich

    2010-01-01

    A graphical-user-interface (GUI) computer program has been developed to facilitate research on the damage caused by highly energetic particles and photons impinging on living organisms. The program brings together, into one computational workspace, computer codes that have been developed over the years, plus codes that will be developed during the foreseeable future, to address diverse aspects of radiation damage. These include codes that implement radiation-track models, codes for biophysical models of breakage of deoxyribonucleic acid (DNA) by radiation, pattern-recognition programs for extracting quantitative information from biological assays, and image-processing programs that aid visualization of DNA breaks. The radiation-track models are based on transport models of interactions of radiation with matter and solution of the Boltzmann transport equation by use of both theoretical and numerical models. The biophysical models of breakage of DNA by radiation include biopolymer coarse-grained and atomistic models of DNA, stochastic- process models of deposition of energy, and Markov-based probabilistic models of placement of double-strand breaks in DNA. The program is designed for use in the NT, 95, 98, 2000, ME, and XP variants of the Windows operating system.

  18. DNA damage induced by the direct effect of radiation

    NASA Astrophysics Data System (ADS)

    Yokoya, A.; Shikazono, N.; Fujii, K.; Urushibara, A.; Akamatsu, K.; Watanabe, R.

    2008-10-01

    We have studied the nature of DNA damage induced by the direct effect of radiation. The yields of single- (SSB) and double-strand breaks (DSB), base lesions and clustered damage were measured using the agarose gel electrophoresis method after exposing to various kinds of radiations to a simple model DNA molecule, fully hydrated closed-circular plasmid DNA (pUC18). The yield of SSB does not show significant dependence on linear energy transfer (LET) values. On the other hand, the yields of base lesions revealed by enzymatic probes, endonuclease III (Nth) and formamidopyrimidine DNA glycosylase (Fpg), which excise base lesions and leave a nick at the damage site, strongly depend on LET values. Soft X-ray photon (150 kVp) irradiation gives a maximum yield of the base lesions detected by the enzymatic probes as SSB and clustered damage, which is composed of one base lesion and proximate other base lesions or SSBs. The clustered damage is visualized as an enzymatically induced DSB. The yields of the enzymatically additional damages strikingly decrease with increasing levels of LET. These results suggest that in higher LET regions, the repair enzymes used as probes are compromised because of the dense damage clustering. The studies using simple plasmid DNA as a irradiation sample, however, have a technical difficulty to detect multiple SSBs in a plasmid DNA. To detect the additional SSBs induced in opposite strand of the first SSB, we have also developed a novel technique of DNA-denaturation assay. This allows us to detect multiply induced SSBs in both strand of DNA, but not induced DSB.

  19. Induced swelling in radiation damaged ZrSiO 4

    NASA Astrophysics Data System (ADS)

    Exarhos, G. J.

    1984-02-01

    A hydrothermal gelation method was used to prepare phase pure polycrystalline ZrSiO 4 which was sintered to 95% theoretical density. Actinide doped samples containing 10 wt% 238Pu were prepared by an analogous procedure and incurred bulk radiation damage through internal alpha-decay processes. Undoped samples were subjected to external irradiation from 5.5 MeV alpha sources, and from a 60Co gamma source. Actinide doped ZrSiO 4 exhibits dose dependent swelling caused by displacement processes leading to ingrowth of amorphous regions. Bulk density and XRD measurements, as a function of dose, showed first order exponential ingrowth behavior similar to that observed in other actinide doped materials. Results are compared with reported data for naturally damaged crystals subjected to significantly lower alpha decay rates. No significant dose rate dependence on damage ingrowth has been observed. Kinetic models for the observed dose dependent swelling are proposed and rate constants for damage ingrowth in synthetic and natural crystals are compared. To study localized damage induced by both external alpha and gamma irradiation, vibrational Raman measurements were obtained for several accumulated doses. Results indicate that the initial stage of damage ingrowth is confined to the silicate sublattice. Vibrational results will be discussed in terms of microstructural changes which result from irradiation.

  20. Damage-tolerant nanotwinned metals with nanovoids under radiation environments

    SciTech Connect

    Chen, Y.; Yu, K. Y.; Liu, Y.; Shao, S.; Wang, H.; Kirk, M. A.; Wang, J.; Zhang, X.

    2015-04-24

    Material performance in extreme radiation environments is central to the design of future nuclear reactors. Radiation induces significant damage in the form of dislocation loops and voids in irradiated materials, and continuous radiation often leads to void growth and subsequent void swelling in metals with low stacking fault energy. Here we show that by using in situ heavy ion irradiation in a transmission electron microscope, pre-introduced nanovoids in nanotwinned Cu efficiently absorb radiation-induced defects accompanied by gradual elimination of nanovoids, enhancing radiation tolerance of Cu. In situ studies and atomistic simulations reveal that such remarkable self-healing capability stems from high density of coherent and incoherent twin boundaries that rapidly capture and transport point defects and dislocation loops to nanovoids, which act as storage bins for interstitial loops. This study describes a counterintuitive yet significant concept: deliberate introduction of nanovoids in conjunction with nanotwins enables unprecedented damage tolerance in metallic materials.

  1. Extreme radiation damage in soil from Mare Fecunditatis.

    NASA Technical Reports Server (NTRS)

    Phakey, P. P.; Price, P. B.

    1972-01-01

    High-voltage electron microscopy has been used to compare radiation effects in micron-size soil grains from the Luna 16 site (Mare Fecunditatis) and the four Apollo landing sites. Radiation damage by heavy solar particles is strikingly greater in the Luna 16 sample than in the other four samples. It is suggested that less movement of the soil at Mare Fecunditatis has taken place, perhaps because of its proximity to the limb and consequent lower exposure to energetic electrons in the earth's magnetospheric tail, which would cause electrostatic agitation of fine particles.

  2. Proton induced radiation damage in fast crystal scintillators

    NASA Astrophysics Data System (ADS)

    Yang, Fan; Zhang, Liyuan; Zhu, Ren-Yuan; Kapustinsky, Jon; Nelson, Ron; Wang, Zhehui

    2016-07-01

    This paper reports proton induced radiation damage in fast crystal scintillators. A 20 cm long LYSO crystal, a 15 cm long CeF3 crystal and four liquid scintillator based sealed quartz capillaries were irradiated by 800 MeV protons at Los Alamos up to 3.3 ×1014 p /cm2. Four 1.5 mm thick LYSO plates were irradiated by 24 GeV protons at CERN up to 6.9 ×1015 p /cm2. The results show an excellent radiation hardness of LYSO crystals against charged hadrons.

  3. Radiation damage studies of detector-compatible Si JFETs

    NASA Astrophysics Data System (ADS)

    Dalla Betta, Gian-Franco; Boscardin, Maurizio; Candelori, Andrea; Pancheri, Lucio; Piemonte, Claudio; Ratti, Lodovico; Zorzi, Nicola

    2007-03-01

    We have largely improved the performance of our detector-compatible Si JFETs by optimizing the fabrication technology. New devices feature thermal noise values close to the theoretical ones, and remarkably low 1/ f noise figures. In view of adopting these JFETs for X-ray imaging and HEP applications, bulk and surface radiation damage tests have been carried out by irradiating single transistors and test structures with neutrons and X-rays. Selected results from static and noise characterization of irradiated devices are discussed in this paper, and the impact of radiation effects on the performance of JFET-based circuits is addressed.

  4. Helium Plasma Damage of Low-k Carbon Doped Silica Film: the Effect of Si Dangling Bonds on the Dielectric Constant

    NASA Astrophysics Data System (ADS)

    Li, Hailing; Wang, Qing; Ba, Dechun

    2014-11-01

    The low-k carbon doped silica film has been modified by radio frequency helium plasma at 5 Pa pressure and 80 W power with subsequent XPS, FTIR and optical emission spectroscopy analysis. XPS data indicate that helium ions have broken Si-C bonds, leading to Si-C scission with C(1s) lost seriously. The Si(2p), O(1s), peak obviously shifted to higher binding energies, indicating an increasingly oxidized Si(2p). FTIR data also show that the silanol formation increased with longer exposure time up to a week. Contrarily, the CH3 stretch, Si-C stretching bond and the ratio of the Si-O-Si cage and Si-O-Si network peak sharply decreased upon exposure to helium plasma. The OES result indicates that monovalent helium ions in plasma play a key role in damaging carbon doped silica film. So it can be concluded that the monovalent helium ions besides VUV photons can break the weak Si-C bonds to create Si dangling bonds and free methyl radicals, and the latter easily reacts with O2 from the atmosphere to generate CO2 and H2O. The bonds change is due to the Si dangling bonds combining with H2O, thereby, increasing the dielectric constant k value.

  5. A stochastic model of radiation-induced bone marrow damage

    SciTech Connect

    Cotlet, G.; Blue, T.E.

    2000-03-01

    A stochastic model, based on consensus principles from radiation biology, is used to estimate bone-marrow stem cell pool survival (CFU-S and stroma cells) after irradiation. The dose response model consists of three coupled first order linear differential equations which quantitatively describe time dependent cellular damage, repair, and killing of red bone marrow cells. This system of differential equations is solved analytically through the use of a matrix approach for continuous and fractionated irradiations. The analytic solutions are confirmed through the dynamical solution of the model equations using SIMULINK. Rate coefficients describing the cellular processes of radiation damage and repair, extrapolated to humans from animal data sets and adjusted for neutron-gamma mixed fields, are employed in a SIMULINK analysis of criticality accidents. The results show that, for the time structures which may occur in criticality accidents, cell survival is established mainly by the average dose and dose rate.

  6. Radiation damage in zircon by high-energy electron beams

    SciTech Connect

    Jiang Nan; Spence, John C. H.

    2009-06-15

    Radiation damage induced by high-energy (200 keV) electron irradiation in zircon has been studied thoroughly using imaging, diffraction, and electron energy-loss spectroscopy techniques in transmission electron microscopy. Both structural and compositional changes during the damage were measured using the above techniques in real time. It was found that the damage was mainly caused by the preferential sputtering of O. The loss of O occurred initially within small sporadic regions with dimension of several nanometers, resulting in the direct transformation of zircon into Zr{sub x}Si{sub y}. These isolated patches gradually connect each other and eventually cover the whole area of the electron beam. These differ from the previous observations either in the self-irradiated natural and synthetic zircon or in ion-beam irradiated thin zircon specimen.

  7. Pluripotent stem cells and DNA damage response to ionizing radiations

    PubMed Central

    Mujoo, Kalpana; Butler, E. Brian; Pandita, Raj K.; Hunt, Clayton R.; Pandita, Tej K.

    2016-01-01

    Pluripotent stem cells (PSCs) hold great promise in regenerative medicine, disease modeling, functional genomics, toxicological studies and cell-based therapeutics due to their unique characteristics of self-renewal and pluripotency. Novel methods for generation of pluripotent stem cells and their differentiation to the specialized cell types such as neuronal cells, myocardial cells, hepatocytes, and beta cells of the pancreas and many other cells of the body are constantly being refined. Pluripotent stem cell derived differentiated cells, including neuronal cells or cardiac cells are ideal for stem cell transplantation as autologous or allogeneic cells from healthy donors due to their minimum risks of rejection. DNA damage induced by ionizing radiation (IR), ultraviolet (UV) light, genotoxic stress, and other intrinsic and extrinsic factors trigger a series of biochemical reactions termed as DNA damage response (DDR). In order to maintain genomic stability, and avoid transmission of mutations into progenitors cells, stem cells have robust DNA damage response signaling – a contrast to somatic cells. Stem cell transplantation may over come the late effects related to radiation. This review will particularly focus on differential DNA damage response between stem cells and derived differentiated cells and the possible pathways that determine such differences. PMID:27332952

  8. Multiscale approach to the physics of radiation damage with ions

    NASA Astrophysics Data System (ADS)

    Surdutovich, Eugene; Solov'yov, Andrey V.

    2014-11-01

    The multiscale approach to the assessment of biodamage resulting upon irradiation of biological media with ions is reviewed, explained and compared to other approaches. The processes of ion propagation in the medium concurrent with ionization and excitation of molecules, transport of secondary products, dynamics of the medium, and biological damage take place on a number of different temporal, spatial and energy scales. The multiscale approach, a physical phenomenon-based analysis of the scenario that leads to radiation damage, has been designed to consider all relevant effects on a variety of scales and develop an approach to the quantitative assessment of biological damage as a result of irradiation with ions. Presently, physical and chemical effects are included in the scenario while the biological effects such as DNA repair are only mentioned. This paper explains the scenario of radiation damage with ions, overviews its major parts, and applies the multiscale approach to different experimental conditions. On the basis of this experience, the recipe for application of the multiscale approach is formulated. The recipe leads to the calculation of relative biological effectiveness.

  9. Pluripotent Stem Cells and DNA Damage Response to Ionizing Radiations.

    PubMed

    Mujoo, Kalpana; Butler, E Brian; Pandita, Raj K; Hunt, Clayton R; Pandita, Tej K

    2016-07-01

    Pluripotent stem cells (PSCs) hold great promise in regenerative medicine, disease modeling, functional genomics, toxicological studies and cell-based therapeutics due to their unique characteristics of self-renewal and pluripotency. Novel methods for generation of pluripotent stem cells and their differentiation to the specialized cell types such as neuronal cells, myocardial cells, hepatocytes and beta cells of the pancreas and many other cells of the body are constantly being refined. Pluripotent stem cell derived differentiated cells, including neuronal cells or cardiac cells, are ideal for stem cell transplantation as autologous or allogeneic cells from healthy donors due to their minimal risk of rejection. Radiation-induced DNA damage, ultraviolet light, genotoxic stress and other intrinsic and extrinsic factors triggers a series of biochemical reactions known as DNA damage response. To maintain genomic stability and avoid transmission of mutations into progenitors cells, stem cells have robust DNA damage response signaling, a contrast to somatic cells. Stem cell transplantation may protect against radiation-induced late effects. In particular, this review focuses on differential DNA damage response between stem cells and derived differentiated cells and the possible pathways that determine such differences. PMID:27332952

  10. Effects of helium ion damage on the two-band superconductivity in MgB2 thin films

    NASA Astrophysics Data System (ADS)

    Greene, L. H.; Park, W. K.; Lu, X.; Moeckly, B.; Singh, R.; Newman, N.; Rowell, J. M.

    2006-03-01

    While the two-band superconductivity in MgB2 has been well established, it remains controversial whether disorder in the Mg and B planes causes enhanced interband scattering, band filling or both. To address this, we have performed electronic transport and point-contact spectroscopy measurements on helium-ion irradiated MgB2 thin films. Two sets of samples are prepared using: a) 1 MeV He^+ ions with uniform doses ranging from 1x10^15 to 1x10^17 ions/cm^2; b) 2 MeV alpha particles with gradient doses. The resistivity (Tc) is observed to increase (decrease) monotonically with increasing dose. The conductance spectra are taken from point-contact junctions between MgB2 thin films and Au tips. The Tc is determined by the onset of enhanced conductance at zero bias. Our preliminary results show that the two gaps tend to merge into one gap with increasing ion damage. Detailed results including Tc vs. energy gap will be presented and discussed in terms of the electronic structure change caused by ion-induced point defects. WKP acknowledges Pavel Krasnochtchekov and Robert Averback for ion irradiation experiments at UIUC. This work is supported by the DoE DEFG02-91ER45439, through the FSMRL and the Center for Microanalysis of Materials at UIUC.

  11. Radiation damage of a glass-bonded zeolite waste form using ion irradiation.

    SciTech Connect

    Allen, T. R.; Storey, B. G.

    1997-12-05

    Glass-bonded zeolite is being considered as a candidate ceramic waste form for storing radioactive isotopes separated from spent nuclear fuel in the electrorefining process. To determine the stability of glass-bonded zeolite under irradiation, transmission electron microscope samples were irradiated using high energy helium, lead, and krypton. The major crystalline phase of the waste form, which retains alkaline and alkaline earth fission products, loses its long range order under both helium and krypton irradiation. The dose at which the long range crystalline structure is lost is about 0.4 dpa for helium and 0.1 dpa for krypton. Because the damage from lead is localized in such a small region of the sample, damage could not be recognized even at a peak damage of 50 dpa. Because the crystalline phase loses its long range structure due to irradiation, the effect on retention capacity needs to be further evaluated.

  12. Radiation damage effects in candidate titanates for Pu disposition: Zirconolite

    NASA Astrophysics Data System (ADS)

    Strachan, D. M.; Scheele, R. D.; Buck, E. C.; Kozelisky, A. E.; Sell, R. L.; Elovich, R. J.; Buchmiller, W. C.

    2008-01-01

    Results from studies of radiation-induced damage from the alpha decay of 238Pu on the density and crystal structure of a nominally phase-pure zirconolite and two other zirconolite-bearing ceramics are discussed. Macro and micro swelling were found to be temperature independent, whereas the density determined with He gas pycnometry was temperature dependent. Approximately 2.6 × 10 18 α/g were needed to render the specimens X-ray amorphous- more to saturate the swelling. Unlike pyrochlore-based ceramics, we did not observe any phase changes associated with storage temperature and damage ingrowth. The forward dissolution rate at a pH value of 2 for material containing essentially all zirconolite is 1.7(4) × 10 -3 g/(m 2 d) with very little pH dependence and no dependence on the amount of radiation-induced damage. Even after the radiation-induced swelling saturated, the specimens remained physically intact with no evidence for microcracking. Thus, the material remains physically a viable material for the disposition of surplus weapons-grade Pu.

  13. Ascorbic acid (AA) metabolism in protection against radiation damage

    SciTech Connect

    Rose, R.C.; Koch, M.J.

    1986-03-05

    The possibility is considered that AA protects tissues against radiation damage by scavenging free radicals that result from radiolysis of water. A physiologic buffer (pH 6.7) was incubated with /sup 14/C-AA and 1 mM thiourea (to slow spontaneous oxidation of AA). Aliquots were assayed by HPLC and scintillation spectrometry to identify the /sup 14/C-label. Samples exposed to Cobalt-60 radiation had a half time of AA decay of < 3 minutes compared with nonirradiated samples (t/sub 1/2/ > 30 minutes) indicating that AA scavenges radiation-induced free radicals and forms the ascorbate free radical (AFR). Pairs of /sup 14/C-AFR disproportionate, with the net effect of /sup 14/C-dehydroascorbic acid formation from /sup 14/C-AA. Having established that AFR result from ionizing radiation in an aqueous solution, the possibility was evaluated that a tissue factor reduces AFR. Cortical tissue from the kidneys of male rats was minced, homogenized in buffer and centrifuged at 8000 xg. The supernatant was found to slow the rate of radiation-induced AA degradation by > 90% when incubated at 23/sup 0/C in the presence of 15 ..mu..M /sup 14/C-AA. Samples of supernatant maintained at 100/sup 0/C for 10 minutes or precipitated with 5% PCA did not prevent radiation-induced AA degradation. AA may have a specific role in scavenging free radicals generated by ionizing radiation and thereby protect body tissues.

  14. Radiative properties of molecular nitrogen ions produced by helium Penning ionization and argon effects

    NASA Technical Reports Server (NTRS)

    Miller, George, III; Song, Kyo-Dong

    1994-01-01

    The development of hypersonic aerospace vehicles requires a better understanding on the thermal and chemical nonequilibrium kinetics of participating species in shock layers. The computational fluid dynamic (CFD) codes developed for such flowfields overestimate the radiation in the spectral region of 300 - 600 nm. A speculation for this overestimation is that inclusion of Ar, CO2, and H2O at the upper atmosphere flight region makes a significant impact on radiative kinetics of molecular nitrogen ions. To define the effects of minority species on the radiative kinetics of N2(+), an experimental setup was made by using the helium Penning ionization. The vibrational and rotational temperature were measured by mapping the vibrational and rotational distributions of N2(+) emission with high spectroscopic resolution and absolute intensity measurements. Measured vibrational temperatures were in the range from 18,000 to 36,000 K, and rotational temperatures were in the range from 300 to 370 K. The irradiance of 391.44 nm line and rotational and vibrational temperatures were analyzed to define argon and CO2 effects on the N2(+) emission. When Ar or CO2 is injected with N2, the rotational temperature did not change. The irradiances were reduced by 34 percent and 78 percent for the 50 percent of mixture of Ar and CO2, respectively. The vibrational temperatures were increased by 24.1 percent and 82.9 percent for the 50 percent of mixture of Ar and CO2, respectively. It appears that there are no significant effects from small concentrations of Ar and CO2 at the upper atmosphere flight region.

  15. Collisional-radiative model of helium microwave discharges at atmospheric pressure

    NASA Astrophysics Data System (ADS)

    Santos, M.; Alves, L. L.; Gadonna, K.; Belmonte, T.

    2011-10-01

    This paper presents a stationary collisional-radiative model to describe the behavior of helium microwave discharges (2.45 GHz), produced in cylindrical geometry (1 mm radius) at atmospheric pressure. The model couples the rate balance equations for the charged particles (electrons, He+ and He2+ions), the He(n <= 6) excited states and the He2*excimers, to the two-term homogeneous and stationary electron Boltzmann equation,. The latter is solved using a coherent set of electron cross sections, adjusted to ensure good predictions of the swarm parameters and the Townsend ionization coefficient. The model was solved for typical 5x1014 cm-3 electron density and 2500 K gas temperature, yielding [He2+]/[He+] ~ 0.92 and [He2*]/[He] ~ 3.4x10-8. Results show also that the He2+ions are produced mainly from the 3-body conversion of He+ ions and lost by the corresponding reverse reaction together with diffusion and dissociative recombination. The He2*is produced by a 3-body reaction involving the 23P states and by the electron-stabilized recombination of He2+and is lost by electron dissociation. This paper presents a stationary collisional-radiative model to describe the behavior of helium microwave discharges (2.45 GHz), produced in cylindrical geometry (1 mm radius) at atmospheric pressure. The model couples the rate balance equations for the charged particles (electrons, He+ and He2+ions), the He(n <= 6) excited states and the He2*excimers, to the two-term homogeneous and stationary electron Boltzmann equation,. The latter is solved using a coherent set of electron cross sections, adjusted to ensure good predictions of the swarm parameters and the Townsend ionization coefficient. The model was solved for typical 5x1014 cm-3 electron density and 2500 K gas temperature, yielding [He2+]/[He+] ~ 0.92 and [He2*]/[He] ~ 3.4x10-8. Results show also that the He2+ions are produced mainly from the 3-body conversion of He+ ions and lost by the corresponding reverse reaction together

  16. Radiation damage effects in CZT drift strip detectors

    NASA Astrophysics Data System (ADS)

    Kuvvetli, Irfan; Budtz-Joergensen, Carl; Korsbech, Uffe; Jensen, H. J.

    2003-03-01

    At DSRI, in collaboration with the cyclotron facility at Copenhagen University Hospital, we have performed a study of radiation effects exposing a 2.7 mm thick CZT drift strip detector to 30 MeV protons. The detector characteristics were evaluated after exposure to a number of dose loads in the range from 2*108 to 60*108 p+/cm2. Even for the highest dose loads, which had a dramatic effect on the spectroscopic performance, we were able to recover the detectors after an appropriate annealing procedure. The radiation damage was studied as function of depth inside the detector material. A numerical model that emulates the physical processes of the charge transport in the CZT detector was used to derive the charge trapping parameter , μτe (the product of charge mobility and trapping time) as function of dose. The analysis showed that the electron trapping increased proportional with the proton dose. The radiation contribution to the electron trapping was found to obey the following relation: (μτe)-1rad =(2.5±0.2)*10-7*Φ (V/cm2) with the proton fluence, Φ in p+/cm2. The trapping depth dependence, however, did not agree well the damage profile calculated using the standard Monte Carlo simulations, TRIM for the proton induced radiation effects. The present results suggest that proton induced nuclear reactions contribute significantly to the radiation damage. Further work will elaborate on these effects. The detector energy resolution was investigated as function of proton dose. It was found that the observed degradation is well explained by the decrease of μτe when the fluctuations of the electron path length are taken into account. The proton irradiation produced In meta stable isotopes in the CZT material. Their decay and production yield as function of depth were analyzed.

  17. Contribution of endogenous and exogenous damage to the total radiation-induced damage in the bacterial spore

    SciTech Connect

    Jacobs, G.P.; Samuni, A.; Czapski, G.

    1980-01-01

    Radical scavengers such as polyethylene glycol 4000 and bovine albumin have been used to define the contribution of exogenous and endogenous damage to the total radiation-induced damage in aqueous buffered suspensions of Bacillus pumilus spores. The results indicate that this damage in the bacterial spore is predominantly endogenous.

  18. [The application of helium-neon laser radiation for the combined treatment of the patients with atrophic rhinitis].

    PubMed

    Sharipov, R A; Sharipova, E R

    2012-01-01

    The objective of the present study was to improve the efficacy of the treatment of the patients presenting with atrophic rhinitis (ozena) of the upper respiratory tract by the application of helium-neon laser radiation. A total of 120 patients aged from 15 to 53 years were treated based at the Department of Otorhinolaryngology, G.G. Kuvatov Republican Clinical Hospital, Ufa. All these patients underwent routine clinical, roentgenological, microbiological, and rheographic examination. The method for the treatment of atrophic rhinitis is described; it includes the application of helium-neon laser radiation in combination with the administration of the purified preparation of liquid polyvalent Klebsiella bacteriophage. The positive results of the treatment by the proposed method were documented in 90% of the patients. PMID:23268248

  19. PREFACE: Radiation Damage in Biomolecular Systems (RADAM07)

    NASA Astrophysics Data System (ADS)

    McGuigan, Kevin G.

    2008-03-01

    The annual meeting of the COST P9 Action `Radiation damage in biomolecular systems' took place from 19-22 June 2007 in the Royal College of Surgeons in Ireland, in Dublin. The conference was structured into 5 Working Group sessions: Electrons and biomolecular interactions Ions and biomolecular interactions Radiation in physiological environments Theoretical developments for radiation damage Track structure in cells Each of the five working groups presented two sessions of invited talks. Professor Ron Chesser of Texas Tech University, USA gave a riveting plenary talk on `Mechanisms of Adaptive Radiation Responses in Mammals at Chernobyl' and the implications his work has on the Linear-No Threshold model of radiation damage. In addition, this was the first RADAM meeting to take place after the Alexander Litvenenko affair and we were fortunate to have one of the leading scientists involved in the European response Professor Herwig Paretzke of GSF-Institut für Strahlenschutz, Neuherberg, Germany, available to speak. The remaining contributions were presented in the poster session. A total of 72 scientific contributions (32 oral, 40 poster), presented by 97 participants from 22 different countries, gave an overview on the current progress in the 5 different subfields. A 1-day pre-conference `Early Researcher Tutorial Workshop' on the same topic kicked off on 19 June attended by more than 40 postgrads, postdocs and senior researchers. Twenty papers, based on these reports, are included in this volume of Journal of Physics: Conference Series. All the contributions in this volume were fully refereed, and they represent a sample of the courses, invited talks and contributed talks presented during RADAM07. The interdisciplinary RADAM07 conference brought together researchers from a variety of different fields with a common interest in biomolecular radiation damage. This is reflected by the disparate backgrounds of the authors of the papers presented in these proceedings

  20. Molecular dynamics modelling of radiation damage in zircon

    NASA Astrophysics Data System (ADS)

    Grechanovsky, A. E.

    2009-04-01

    Zircon (ZrSiO4) is among actinide-bearing phases which has been proposed as a crystalline confinement matrix for nuclear waste management, especially for weapon-grade plutonium and UO2 spent fuel in the USA. Zircon is also widely used in geochronology. But, with accumulating α-decay damage, zircon undergoes a radiation induced transition to an amorphous (or metamict) state. So, in the present work molecular dynamics simulations (MD simulations) of zircon structure have been performed to study radiation damage in zircon. In this technique, one simulates the propagation of an energetic particle in a system of atoms interacting via model potentials, by integrating the Newton equations of motion. Author has used version 3.09 of the DL_POLY molecular simulation package. Zircon structure containing 181944 atoms (19x19x21 unit cells) was equilibrated at 300 K for 10 ps, and one Zr atom (usually called the primary knock-on atom, PKA) was given a velocity corresponding to an implantation energy of about 20 keV. MD simulations were performed in the microcanonical ensemble that is under conditions of constant particle number, volume and energy. Results of the MD simulations show that the number of interstitials is equal to 840 atoms. This is very close (4000-5000 atoms for 70 keV recoil atom 234Th) to what is measured in the diffuse x-ray scattering and NMR experiments on amorphous metamict samples (damaged by natural irradiation) of geological age. It has been shown that the damaged structure contains several depleted regions with characteristic sized up to 2,5 nm after single event and up to 4,5 nm after three overlapping events. Furthermore, these events produce channels of depleted matter between the overlapping damaged regions. These channels provide a high-diffusivity path for radiogenic Pb (percolation effect). Loss of radiogenic Pb may result in to incorrect dating of rocks.

  1. MD simulations of phase stability of PuGa alloys: Effects of primary radiation defects and helium bubbles

    DOE PAGESBeta

    Dremov, V. V.; Sapozhnikov, F. A.; Ionov, G. V.; Karavaev, A. V.; Vorobyova, M. A.; Chung, B. W.

    2013-05-14

    We present classical molecular dynamics (MD) with Modified Embedded Atom Model (MEAM) simulations to investigate the role of primary radiation defects and radiogenic helium as factors affecting the phase stability of PuGa alloys in cooling–heating cycles at ambient pressure. The models of PuGa alloys equilibrated at ambient conditions were subjected to cooling–heating cycles in which they were initially cooled down to 100 K and then heated up to 500 K at ambient pressure. The rate of temperature change in the cycles was 10 K/ns. The simulations showed that the initial FCC phase of PuGa alloys undergo polymorphous transition in coolingmore » to a lower symmetry α'-phase. All the alloys undergo direct and reverse polymorphous transitions in the cooling–heating cycles. The alloys containing vacancies shift in both transitions to lower temperatures relative to the defect-free alloys. The radiogenic helium has much less effect on the phase stability compared to that of primary radiation defects (in spite of the fact that helium concentration is twice of that for the primary radiation defects). Lastly, this computational result agrees with experimental data on unconventional stabilization mechanism of PuGa alloys.« less

  2. MD simulations of phase stability of PuGa alloys: Effects of primary radiation defects and helium bubbles

    SciTech Connect

    Dremov, V. V.; Sapozhnikov, F. A.; Ionov, G. V.; Karavaev, A. V.; Vorobyova, M. A.; Chung, B. W.

    2013-05-14

    We present classical molecular dynamics (MD) with Modified Embedded Atom Model (MEAM) simulations to investigate the role of primary radiation defects and radiogenic helium as factors affecting the phase stability of PuGa alloys in cooling–heating cycles at ambient pressure. The models of PuGa alloys equilibrated at ambient conditions were subjected to cooling–heating cycles in which they were initially cooled down to 100 K and then heated up to 500 K at ambient pressure. The rate of temperature change in the cycles was 10 K/ns. The simulations showed that the initial FCC phase of PuGa alloys undergo polymorphous transition in cooling to a lower symmetry α'-phase. All the alloys undergo direct and reverse polymorphous transitions in the cooling–heating cycles. The alloys containing vacancies shift in both transitions to lower temperatures relative to the defect-free alloys. The radiogenic helium has much less effect on the phase stability compared to that of primary radiation defects (in spite of the fact that helium concentration is twice of that for the primary radiation defects). Lastly, this computational result agrees with experimental data on unconventional stabilization mechanism of PuGa alloys.

  3. Radiation damage of the HEAO C-1 germanium detectors

    NASA Technical Reports Server (NTRS)

    Mahoney, W. A.; Ling, J. C.; Jacobson, A. S.

    1981-01-01

    The effects of radiation damage from proton bombardment of the four HEAO C-1 high purity germanium detectors have been measured and compared to predictions. Because of the presence of numerous gamma-ray lines in the detector background spectra and because of the relatively long exposure time of the HEAO 3 satellite to cosmic-ray and trapped protons, it has been possible to measure both the energy and time dependence of radiation damage. After 100 d in orbit, each of the four detectors has been exposed to approximately 3 x 10 to the 7th protons/sq cm, and the average energy resolution at 1460 keV had degraded from 3.2 keV fwhm to 8.6 keV fwhm. The lines were all broadened to the low energy side although the line profile was different for each of the four detectors. The damage-related contribution to the degradation in energy resolution was found to be linear in energy and proton influence.

  4. In Situ Measurement of Radiation Damage in Scintillating Fibers

    NASA Astrophysics Data System (ADS)

    Ziegler, Ar.; Holm, U.; Latuske, N.; Wick, K.; Zoufal, T.

    2002-11-01

    The radiation induced degradation of the optical transmission of the fibers SCSF-38M, SCSF-81M (Kuraray), BCF-60 and BCF-98 (Bicron) with polystyrene core (PS) was studied. During and after irradiation with a 100 kV X-ray source, a 137Cs source and a 60Co source the effects depend on the fiber type: (1) The permanent damage for BCF-98 (clear PS) is smaller than for the scintillators. (2) The BCF-60 is radiation harder than the other two scintillators but very light sensitive. Temperature treatments (up to 68°C) of SCSF-38M, without irradiation showed a transmission loss which clearly rises with the increasing temperature. This accelerated ageing phenomenon does not recover and the fiber is permanently damaged. In an additional experiment it was studied whether the transmission damage can be influenced by short illuminations with visible light during and after irradiation. For SCSF-38M a strong reduction of the permanent induced absorption remaining after the end of the recovery process was observed.

  5. Radiation damage in PVT (Polyvinyltoluene) induced by energetic ions

    NASA Astrophysics Data System (ADS)

    Torrisi, L.

    Polyvinyltoluene (PVT) is an organic polymer employed as base material for many plastic scintillators useful to detect charged particles. Radiation damage in PVT is investigated irradiating the polymer in vacuum with different ion beams (H+, He+, N+ and Ar+) as a function of their ion stopping power. The structural modifications indced in the polymer are deduced by monitoring in situ, during the ion irradiation, the molecular desorption from the polymer by a highly sensitive mass-quadrupole spectrometer. The desorbed molecules are detected in the mass range 1-100 amu and the chemical yields are measured with respect to the calibrated gas leaks. Main emitted species are H2, C2H2 and C3H5, the yields of which strongly depend on the ion stopping power. As will be discussed, the investigation of radiation damage in PVT permits to extend the results to the damage undergone by plastic scintillators during the detection of charged particles at high energy, such as protons of 10-100 MeV, an energy range useful in nuclear physics and in proton-therapy.

  6. Influence of radiation damage on xenon diffusion in silicon carbide

    NASA Astrophysics Data System (ADS)

    Friedland, E.; Gärtner, K.; Hlatshwayo, T. T.; van der Berg, N. G.; Thabethe, T. T.

    2014-08-01

    Diffusion of xenon in poly and single crystalline silicon carbide and the possible influence of radiation damage on it are investigated. For this purpose 360 keV xenon ions were implanted in commercial 6H-SiC and CVD-SiC wafers at room temperature, 350 °C and 600 °C. Width broadening of the implantation profiles and xenon retention during isochronal and isothermal annealing up to temperatures of 1500 °C was determined by RBS-analysis, whilst in the case of 6H-SiC damage profiles were simultaneously obtained by α-particle channelling. No diffusion or xenon loss was detected in the initially amorphized and eventually recrystallized surface layer of cold implanted 6H-SiC during annealing up to 1200 °C. Above that temperature serious erosion of the implanted surface occurred, which made any analysis impossible. No diffusion or xenon loss is detected in the hot implanted 6H-SiC samples during annealing up to 1400 °C. Radiation damage dependent grain boundary diffusion is observed at 1300 °C in CVD-SiC.

  7. Radiation damage and derivatization in macromolecular crystallography: a structure factor’s perspective

    PubMed Central

    Owen, Robin L.; Sherrell, Darren A.

    2016-01-01

    During, or even after, data collection the presence and effects of radiation damage in macromolecular crystallography may not always be immediately obvious. Despite this, radiation damage is almost always present, with site-specific damage occurring on very short time (dose) scales well before global damage becomes apparent. A result of both site-specific radiation damage and derivatization is a change in the relative intensity of reflections. The size and approximate rate of onset of X-ray-induced transformations is compared with the changes expected from derivatization, and strategies for minimizing radiation damage are discussed. PMID:26960125

  8. Recent Advances in Understanding Radiation Damage in Reactor Cavity Concrete

    SciTech Connect

    Rosseel, Thomas M; Field, Kevin G; Le Pape, Yann; Remec, Igor; Giorla, Alain B; Wall, Dr. James Joseph

    2015-01-01

    License renewal up to 60 years and the possibility of subsequent license renewal to 80 years has resulted in a renewed focus on long-term aging of materials at nuclear power plants (NPPs) including concrete. Large irreplaceable sections of most nuclear generating stations include concrete. The Expanded Materials Degradation Analysis, jointly performed by the Department of Energy, the Nuclear Regulatory Commission and Nuclear Industry, identified the urgent need to develop a consistent knowledge base on irradiation effects in concrete (Graves et al., (2014)). Much of the historical mechanical performance data of irradiated concrete (Hilsdorf et al., (1978)) does not accurately reflect typical radiation conditions in NPPs or conditions out to 60 or 80 years of radiation exposure (Kontani et al., (2011)). To address these potential gaps in the knowledge base, the Electric Power Research Institute and Oak Ridge National Laboratory, are working to better understand radiation damage as a degradation mechanism. This paper outlines recent progress toward: 1) assessing the radiation environment in concrete biological shields and defining the upper bound of the neutron and gamma dose levels expected in the biological shield for extended operation, and estimating adsorbed dose, 2) evaluating opportunities to harvest and test irradiated concrete from international NPPs, 3) evaluating opportunities to irradiate prototypical concrete and its components under accelerated neutron and gamma dose levels to establish conservative bounds and inform damage models, 4) developing improved models to enhance the understanding of the effects of radiation on concrete and 5) establishing an international collaborative research and information exchange effort to leverage capabilities and knowledge including developing cooperative test programs to improve confidence in data obtained from various concretes and from accelerated irradiation experiments.

  9. Torin2 Suppresses Ionizing Radiation-Induced DNA Damage Repair.

    PubMed

    Udayakumar, Durga; Pandita, Raj K; Horikoshi, Nobuo; Liu, Yan; Liu, Qingsong; Wong, Kwok-Kin; Hunt, Clayton R; Gray, Nathanael S; Minna, John D; Pandita, Tej K; Westover, Kenneth D

    2016-05-01

    Several classes of inhibitors of the mammalian target of rapamycin (mTOR) have been developed based on its central role in sensing growth factor and nutrient levels to regulate cellular metabolism. However, its ATP-binding site closely resembles other phosphatidylinositol 3-kinase-related kinase (PIKK) family members, resulting in reactivity with these targets that may also be therapeutically useful. The ATP-competitive mTOR inhibitor, Torin2, shows biochemical activity against the DNA repair-associated proteins ATM, ATR and DNA-PK, which raises the possibility that Torin2 and related compounds might radiosensitize cancerous tumors. In this study Torin2 was also found to enhance ionizing radiation-induced cell killing in conditions where ATM was dispensable, confirming the requirement for multiple PIKK targets. Moreover, Torin2 did not influence the initial appearance of γ-H2AX foci after irradiation but significantly delayed the disappearance of radiation-induced γ-H2AX foci, indicating a DNA repair defect. Torin2 increased the number of radiation-induced S-phase specific chromosome aberrations and reduced the frequency of radiation-induced CtIP and Rad51 foci formation, suggesting that Torin2 works by blocking homologous recombination (HR)-mediated DNA repair resulting in an S-phase specific DNA repair defect. Accordingly, Torin2 reduced HR-mediated repair of I-Sce1-induced DNA damage and contributed to replication fork stalling. We conclude that radiosensitization of tumor cells by Torin2 is associated with disrupting ATR- and ATM-dependent DNA damage responses. Our findings support the concept of developing combination cancer therapies that incorporate ionizing radiation therapy and Torin2 or compounds with similar properties. PMID:27135971

  10. Absolute Measurements of Radiation Damage in Nanometer Thick Films

    PubMed Central

    Alizadeh, Elahe; Sanche, Léon

    2013-01-01

    We address the problem of absolute measurements of radiation damage in films of nanometer thicknesses. Thin films of DNA (~ 2–160nm) are deposited onto glass substrates and irradiated with varying doses of 1.5 keV X-rays under dry N2 at atmospheric pressure and room temperature. For each different thickness, the damage is assessed by measuring the loss of the supercoiled configuration as a function of incident photon fluence. From the exposure curves, the G-values are deduced, assuming that X-ray photons interacting with DNA, deposit all of their energy in the film. The results show that the G-value (i.e., damage per unit of deposited energy) increases with film thickness and reaches a plateau at 30±5 nm. This thickness dependence provides a correction factor to estimate the actual G-value for films with thicknesses below 30nm thickness. Thus, the absolute values of damage can be compared with that of films of any thickness under different experimental conditions. PMID:22562941

  11. The role of nickel in radiation damage of ferritic alloys

    DOE PAGESBeta

    Osetskiy, Yury N.; Anento, Napoleon; Serra, Anna; Terentyev, Dmitry

    2014-11-26

    According to the modern theory damage evolution under neutron irradiation depends on the fraction of self interstitial atoms (SIAs) produced in the form of one-dimensionally (1-D) glissile clusters. These clusters, having a low interaction cross-section with other defects, sink mainly on grain boundaries and dislocations creating the so-called production bias. It is known empirically that addition of certain alloying elements affect many radiation effects, including swelling, however the mechanisms are unknown in many cases. In this paper we report the results of an extensive multi-technique atomistic level modeling of SIA clusters mobility in bcc Fe-Ni alloys with Ni content frommore » 0.8 to 10 at.%. We have found that Ni interacts strongly with periphery of clusters affecting their mobility. The total effect is defined by all Ni atoms interacting with the cluster at the same time and can be significant even in low-Ni alloys. Thus 1nm (37SIAs) cluster is practically immobile at T < 500K in the Fe-0.8at.% Ni alloy. Increasing cluster size and Ni content enhance cluster immobilization. Furthermore, this effect should have quite broad consequences in swelling rate, matrix damage accumulation, radiation induced hardening, etc. and the results obtained help in better understanding and prediction of radiation effects in Fe-Ni ferritic alloys.« less

  12. The evaluation of radiation damage parameter for CVD diamond

    NASA Astrophysics Data System (ADS)

    Grilj, V.; Skukan, N.; Jakšić, M.; Pomorski, M.; Kada, W.; Kamiya, T.; Ohshima, T.

    2016-04-01

    There are a few different phenomenological approaches that aim to track the dependence of signal height in irradiated solid state detectors on the fluence of damaging particles. However, none of them are capable to provide a unique radiation hardness parameter that would reflect solely the material capability to withstand high radiation environment. To extract such a parameter for chemical vapor deposited (CVD) diamond, two different diamond detectors were irradiated with proton beams in MeV energy range and subjected afterwards to ion beam induced charge (IBIC) analysis. The change in charge collection efficiency (CCE) due to defects produced was investigated in context of a theoretical model that was developed on the basis of the adjoint method for linearization of the continuity equations of electrons and holes. Detailed modeling of measured data resulted with the first known value of the kσ product for diamond, where k represents the number of charge carriers' traps created per one simulated primary lattice vacancy and σ represents the charge carriers' capture cross section. As discussed in the text, this product could be considered as a true radiation damage parameter.

  13. Radiation Damages in Aluminum Alloy SAV-1 under Neutron Irradiation

    NASA Astrophysics Data System (ADS)

    Salikhbaev, Umar; Akhmedzhanov, Farkhad; Alikulov, Sherali; Baytelesov, Sapar; Boltabaev, Azizbek

    2016-05-01

    The aim of this work was to study the effect of neutron irradiation on the kinetics of radiation damages in the SAV-1 alloy, which belongs to the group of aluminum alloys of the ternary system Al-Mg-Si. For fast-neutron irradiation by different doses up to fluence 1019 cm-2 the SAV-1 samples were placed in one of the vertical channels of the research WWR type reactor (Tashkent). The temperature dependence of the electrical resistance of the alloy samples was investigated in the range 290 - 490 K by the four-compensation method with an error about 0.1%. The experimental results were shown that at all the temperatures the dependence of the SAV-1 alloy resistivity on neutron fluence was nonlinear. With increasing neutron fluence the deviation from linearity and the growth rate of resistivity with temperature becomes more appreciable. The observed dependences are explained by means of martensitic transformations and the radiation damages in the studied alloy under neutron irradiation. The mechanisms of radiation modification of the SAV-1 alloy structure are discussed.

  14. A radiation damage repair model for normal tissues

    NASA Astrophysics Data System (ADS)

    Partridge, Mike

    2008-07-01

    A cellular Monte Carlo model describing radiation damage and repair in normal epithelial tissues is presented. The deliberately simplified model includes cell cycling, cell motility and radiation damage response (cell cycle arrest and cell death) only. Results demonstrate that the model produces a stable equilibrium system for mean cell cycle times in the range 24-96 h. Simulated irradiation of these stable equilibrium systems produced a range of responses that are shown to be consistent with experimental and clinical observation, including (i) re-epithelialization of radiation-induced lesions by a mixture of cell migration into the wound and repopulation at the periphery; (ii) observed radiosensitivity that is quantitatively consistent with both rate of induction of irreparable DNA lesions and, independently, with the observed acute oral and pharyngeal mucosal reactions to radiotherapy; (iii) an observed time between irradiation and maximum toxicity that is consistent with experimental data for skin; (iv) quantitatively accurate predictions of low-dose hyper-radiosensitivity; (v) Gomperzian repopulation for very small lesions (~2000 cells) and (vi) a linear rate of re-epithelialization of 5-10 µm h-1 for large lesions (>15 000 cells).

  15. The role of nickel in radiation damage of ferritic alloys

    SciTech Connect

    Osetskiy, Yury N.; Anento, Napoleon; Serra, Anna; Terentyev, Dmitry

    2014-11-26

    According to the modern theory damage evolution under neutron irradiation depends on the fraction of self interstitial atoms (SIAs) produced in the form of one-dimensionally (1-D) glissile clusters. These clusters, having a low interaction cross-section with other defects, sink mainly on grain boundaries and dislocations creating the so-called production bias. It is known empirically that addition of certain alloying elements affect many radiation effects, including swelling, however the mechanisms are unknown in many cases. In this paper we report the results of an extensive multi-technique atomistic level modeling of SIA clusters mobility in bcc Fe-Ni alloys with Ni content from 0.8 to 10 at.%. We have found that Ni interacts strongly with periphery of clusters affecting their mobility. The total effect is defined by all Ni atoms interacting with the cluster at the same time and can be significant even in low-Ni alloys. Thus 1nm (37SIAs) cluster is practically immobile at T < 500K in the Fe-0.8at.% Ni alloy. Increasing cluster size and Ni content enhance cluster immobilization. Furthermore, this effect should have quite broad consequences in swelling rate, matrix damage accumulation, radiation induced hardening, etc. and the results obtained help in better understanding and prediction of radiation effects in Fe-Ni ferritic alloys.

  16. Retinal damage from long-term exposure to laser radiation.

    PubMed

    Gibbons, W D; Allen, R G

    1977-06-01

    The maculae of rhesus monkeys were exposed to an argon-ion lazer operated in the TEM00 continuous wave mode at a wavelength of 514.5 nm. Both ophthalmoscopic and histopathologic evaluations of exposure sites were obtained. Threshold (ED50) values were obtained for 0.5, 5, 30, 120, and 1,000 sec. exposure times. Presence of minimum visible lesions was assessed ophthalmoscopically at both 1 hour and 24 hours after exposure. With increasing exposure times, a 24 hr. lesion-appearance criterion resulted in ED50 values too low to be consistent with a thermal damage mechanism. In contrast, exposure to neodymium laser radiation at a 1,060 nm. wavelength for 120 sec. produced only ED50 values consistent with those associated with thermal injury. These results suggest that the damage mechanisms for long-duration exposures to visible light may involve photochemical processes initiated by the interaction of visible light with the retinal photopigments. PMID:405344

  17. A Mathematical Model for Estimating Biological Damage Caused by Radiation

    NASA Astrophysics Data System (ADS)

    Manabe, Yuichiro; Ichikawa, Kento; Bando, Masako

    2012-10-01

    We propose a mathematical model for estimating biological damage caused by low-dose irradiation. We understand that the linear non threshold (LNT) hypothesis is realized only in the case of no recovery effects. In order to treat the realistic living objects, our model takes into account various types of recovery as well as proliferation mechanism, which may change the resultant damage, especially for the case of lower dose rate irradiation. It turns out that the lower the radiation dose rate, the safer the irradiated system of living object (which is called symbolically ``tissue'' hereafter) can have chances to survive, which can reproduce the so-called dose and dose-rate effectiveness factor (DDREF).

  18. Analytical modeling for gamma radiation damage on silicon photodiodes

    NASA Astrophysics Data System (ADS)

    Jafari, H.; Feghhi, S. A. H.

    2016-04-01

    Radiation-induced damage in PIN silicon photodiode induces degradation of the photodiode parameters. In this work, by presenting an analytical model, the effect of gamma dose on the dark current in a PIN photodiode array was investigated. Geant4 was used to obtain the damage constant as a result of primary incident particle fluence and NIEL distribution calculations. Experimental measurements as well as numerical simulation of the semiconductor with ATLAS were carried out to verify and parameterize the analytical model calculations. A reasonable agreement has been found between analytical results and experimental data for BPX65 silicon photodiodes irradiated by a Co-60 gamma source at total doses up to 500 krad under different reverse voltages. Moreover, the results showed that the dark current of each photodiode array pixel has considerably increased by gamma dose irradiation.

  19. Soft X-Ray Microscopy Radiation Damage On Fixed Cells Investigated With Synchrotron Radiation FTIR Microscopy

    NASA Astrophysics Data System (ADS)

    Gianoncelli, A.; Vaccari, L.; Kourousias, G.; Cassese, D.; Bedolla, D. E.; Kenig, S.; Storici, P.; Lazzarino, M.; Kiskinova, M.

    2015-05-01

    Radiation damage of biological samples remains a limiting factor in high resolution X-ray microscopy (XRM). Several studies have attempted to evaluate the extent and the effects of radiation damage, proposing strategies to minimise or prevent it. The present work aims to assess the impact of soft X-rays on formalin fixed cells on a systematic manner. The novelty of this approach resides on investigating the radiation damage not only with XRM, as often reported in relevant literature on the topic, but by coupling it with two additional independent non-destructive microscopy methods: Atomic Force Microscopy (AFM) and FTIR Microscopy (FTIRM). Human Embryonic Kidney 293 cells were exposed to different radiation doses at 1 keV. In order to reveal possible morphological and biochemical changes, the irradiated cells were systematically analysed with AFM and FTIRM before and after. Results reveal that while cell morphology is not substantially affected, cellular biochemical profile changes significantly and progressively when increasing dose, resulting in a severe breakdown of the covalent bonding network. This information impacts most soft XRM studies on fixed cells and adds an in-depth understanding of the radiation damage for developing better prevention strategies.

  20. Soft X-Ray Microscopy Radiation Damage On Fixed Cells Investigated With Synchrotron Radiation FTIR Microscopy

    PubMed Central

    Gianoncelli, A.; Vaccari, L.; Kourousias, G.; Cassese, D.; Bedolla, D. E.; Kenig, S.; Storici, P.; Lazzarino, M.; Kiskinova, M.

    2015-01-01

    Radiation damage of biological samples remains a limiting factor in high resolution X-ray microscopy (XRM). Several studies have attempted to evaluate the extent and the effects of radiation damage, proposing strategies to minimise or prevent it. The present work aims to assess the impact of soft X-rays on formalin fixed cells on a systematic manner. The novelty of this approach resides on investigating the radiation damage not only with XRM, as often reported in relevant literature on the topic, but by coupling it with two additional independent non-destructive microscopy methods: Atomic Force Microscopy (AFM) and FTIR Microscopy (FTIRM). Human Embryonic Kidney 293 cells were exposed to different radiation doses at 1 keV. In order to reveal possible morphological and biochemical changes, the irradiated cells were systematically analysed with AFM and FTIRM before and after. Results reveal that while cell morphology is not substantially affected, cellular biochemical profile changes significantly and progressively when increasing dose, resulting in a severe breakdown of the covalent bonding network. This information impacts most soft XRM studies on fixed cells and adds an in-depth understanding of the radiation damage for developing better prevention strategies. PMID:25974639

  1. Radiation damage and annealing of amorphous silicon solar cells

    NASA Technical Reports Server (NTRS)

    Byvik, C. E.; Slemp, W. S.; Smith, B. T.; Buoncristiani, A. M.

    1984-01-01

    Amorphous silicon solar cells were irradiated with 1 MeV electrons at the Space Environmental Effects Laboratory of the NASA Langley Research Center. The cells accumulated a total fluence of 10 to the 14th, 10 to the 15th, and 10 to the 16th electrons per square centimeter and exhibited increasing degradation with each irradiation. This degradation was tracked by evaluating the I-V curves for AM0 illumination and the relative spectral response. The observed radiation damage was reversed following an anneal of the cells under vacuum at 200 C for 2 hours.

  2. Effects of radiation damage on the silicon lattice

    NASA Technical Reports Server (NTRS)

    Dumas, Katherine A.; Lowry, Lynn; Russo, O. Louis

    1987-01-01

    Silicon was irradiated with both proton and electron particle beams in order to investigate changes in the structural and optical properties of the lattice as a result of the radiation damage. Lattice expansions occurred when large strain fields (+0.34 percent) developed after 1- and 3-MeV proton bombardment. The strain was a factor of three less after 1-MeV electron irradiation. Average increases of approximately 22 meV in the 3.46-eV interband energy gap and 14 meV in the Lorentz broadening parameter were measured after the electron irradiation.

  3. A new mechanism for radiation damage processes in alkali halides

    NASA Astrophysics Data System (ADS)

    Dubinko, V. I.; Turkin, A. A.; Vainshtein, D. I.; den Hartog, H. W.

    1999-12-01

    We present a theory of radiation damage formation in alkali halides based on a new mechanism of dislocation climb, which involves the production of VF centers (self-trapped hole neighboring a cation vacancy) as a result of the absorption of H centers of dislocation lines. We consider the evolution of all experimentally observed extended defects: metal colloids, gas bubbles, and vacancy voids. Voids are shown to arise and grow large due to the reaction between F and VF centers at the surface of halogen bubbles. Voids can ignite a back reaction between the radiolytic products resulting in decomposition of the irradiated material.

  4. Protection of radiation detectors from fast neutron damage

    SciTech Connect

    Kronenberg, S.

    1984-01-30

    A circuit for biasing a solid state crystal used as a radiation detector in which the passage of the initial gamma ray pulse from the explosion of a nearby tactical nuclear weapon is utilized to temporarily remove the bias from said crystal for a time sufficient to permit the fast neutron pulse from the same explosion to pass by without permanently damaging the counter crystal. The circuit comprises an RC circuit between the bias supply and the crystal with a reverse biased diode across the capacitor.

  5. Simulation of neutron radiation damage in silicon semiconductor devices.

    SciTech Connect

    Shadid, John Nicolas; Hoekstra, Robert John; Hennigan, Gary Lee; Castro, Joseph Pete Jr.; Fixel, Deborah A.

    2007-10-01

    A code, Charon, is described which simulates the effects that neutron damage has on silicon semiconductor devices. The code uses a stabilized, finite-element discretization of the semiconductor drift-diffusion equations. The mathematical model used to simulate semiconductor devices in both normal and radiation environments will be described. Modeling of defect complexes is accomplished by adding an additional drift-diffusion equation for each of the defect species. Additionally, details are given describing how Charon can efficiently solve very large problems using modern parallel computers. Comparison between Charon and experiment will be given, as well as comparison with results from commercially-available TCAD codes.

  6. Multiscale physics of ion-induced radiation damage

    NASA Astrophysics Data System (ADS)

    Surdutovich, Eugene; Solov'yov, Andrey V.

    2013-06-01

    A multiscale approach to the physics of ion-beam cancer therapy, an approach suggested in order to understand the interplay of a large number of phenomena involved in radiation damage scenario occurring on a range of temporal, spatial, and energy scales, is being reviewed. The scenario is described along with a variety of effects that take place on different temporal, spatial, and energy scales and play major roles in the scenario of interaction of ions with tissue. The understanding of these effects leads to a quantitative assessment of relative biological effectiveness that relates the physical quantities, such as dose, to the biological values, such as the probability of cell survival.

  7. The energy dependence of refractory metals and alloys radiation damageability

    NASA Astrophysics Data System (ADS)

    Mukashev, K. M.; Umarov, F. F.

    2015-04-01

    In this work the systematical investigation of the radiation defects distribution profile energy dependence in three different materials - tantalum, molybdenum and 10X18H10T-VD stainless steel irradiated by high energy protons has been performed. It has been shown that in stainless steel and tantalum, independently of proton energy, the vacancy complexes related by configuration appear which are described by the slightly expressed elastic channel. The defects are recovered in one annealing stage with different migration activation energy. At the same time the molybdenum radiation damageability is composed of two components in each of which its specified defects formation mechanism takes action. For high energy protons the inelastic channel of interaction is the basic and subcascades appearance is created by primary knocked-on atoms of considerable energies. For low energy protons the processes of elastic interaction with lattice atoms and atomic hydrogen in the end of run creation are major.

  8. Molecular dynamics simulation of radiation damage cascades in diamond

    SciTech Connect

    Buchan, J. T.; Robinson, M.; Christie, H. J.; Roach, D. L.; Ross, D. K.; Marks, N. A.

    2015-06-28

    Radiation damage cascades in diamond are studied by molecular dynamics simulations employing the Environment Dependent Interaction Potential for carbon. Primary knock-on atom (PKA) energies up to 2.5 keV are considered and a uniformly distributed set of 25 initial PKA directions provide robust statistics. The simulations reveal the atomistic origins of radiation-resistance in diamond and provide a comprehensive computational analysis of cascade evolution and dynamics. As for the case of graphite, the atomic trajectories are found to have a fractal-like character, thermal spikes are absent and only isolated point defects are generated. Quantitative analysis shows that the instantaneous maximum kinetic energy decays exponentially with time, and that the timescale of the ballistic phase has a power-law dependence on PKA energy. Defect recombination is efficient and independent of PKA energy, with only 50% of displacements resulting in defects, superior to graphite where the same quantity is nearly 75%.

  9. Medicinal protection with Chinese herb-compound against radiation damage

    SciTech Connect

    Zhang, R.J.; Qian, J.K.; Yang, G.H.; Wang, B.Z.; Wen, X.L. )

    1990-08-01

    Experiments were carried out on mice and the subjects irradiated for cancer therapy to evaluate the protective efficacy of a Chinese medicinal herb-compound (CMHC). The lethality and the degree of leucopenia caused by radiation in mice medicated with CMHC were significantly less in comparison with control mice (p less than 0.01 and p less than 0.001, respectively). CMHC significantly improved the WBC and the thrombocytes in irradiated workers (p less than 0.01 and p less than 0.001, respectively). The WBC count of 40 patients under radiotherapy while treated with CMHC recovered from 3450 +/- 77/c.mm to 5425 +/- 264/c.mm (p less than 0.001); whereas, in the control group, without any medication, the WBC count dropped significantly (p less than 0.001). Our results revealed the applicabilities of CMHC in protection against radiation damage in spaceflight and in other fields.

  10. Radiation-induced lung damage: dose-time-fractionation considerations.

    PubMed

    Van Dyk, J; Mah, K; Keane, T J

    1989-01-01

    The comparison of different dose-time-fractionation schedules requires the use of an isoeffect formula. In recent years, the NSD isoeffect formula has been heavily criticized. In this report, we consider an isoeffect formula which is specifically developed for radiation-induced lung damage. The formula is based on the linear-quadratic model and includes a factor for overall treatment time. The proposed procedures allow for the simultaneous derivation of an alpha/beta ratio and a gamma/beta time factor. From animal data in the literature, the derived alpha/beta and gamma/beta ratios for acute lung damage are 5.0 +/- 1.0 Gy and 2.7 +/- 1.4 Gy2/day respectively, while for late damage the suggested values are 2.0 Gy and 0.0 Gy2/day. Data from two clinical studies, one prospective and the other retrospective, were also analysed and corresponding alpha/beta and gamma/beta ratios were determined. For the prospective clinical study, with a limited range of doses per fraction, the resultant alpha/beta and gamma/beta ratios were 0.9 +/- 2.6 Gy and 2.6 +/- 2.5 Gy2/day. The combination of the retrospective and prospective data yielded alpha/beta and gamma/beta ratios of 3.3 +/- 1.5 Gy and 2.4 +/- 1.5 Gy2/day, respectively. One potential advantage of this isoeffect formalism is that it might possibly be applied to both acute and late lung damage. The results of this formulation for acute lung damage indicate that time-dependent effects such as slow repair or proliferation might be more important in determining isoeffect doses than previously predicted by the estimated single dose (ED) formula. Although we present this as an alternative approach, we would caution against its clinical use until its applicability has been confirmed by additional clinical data. PMID:2928557

  11. Role of Oxidative Damage in Radiation-Induced Bone Loss

    NASA Technical Reports Server (NTRS)

    Schreurs, Ann-Sofie; Alwood, Joshua S.; Limoli, Charles L.; Globus, Ruth K.

    2014-01-01

    During prolonged spaceflight, astronauts are exposed to both microgravity and space radiation, and are at risk for increased skeletal fragility due to bone loss. Evidence from rodent experiments demonstrates that both microgravity and ionizing radiation can cause bone loss due to increased bone-resorbing osteoclasts and decreased bone-forming osteoblasts, although the underlying molecular mechanisms for these changes are not fully understood. We hypothesized that excess reactive oxidative species (ROS), produced by conditions that simulate spaceflight, alter the tight balance between osteoclast and osteoblast activities, leading to accelerated skeletal remodeling and culminating in bone loss. To test this, we used the MCAT mouse model; these transgenic mice over-express the human catalase gene targeted to mitochondria, the major organelle contributing free radicals. Catalase is an anti-oxidant that converts reactive species, hydrogen peroxide into water and oxygen. This animal model was selected as it displays extended lifespan, reduced cardiovascular disease and reduced central nervous system radio-sensitivity, consistent with elevated anti-oxidant activity conferred by the transgene. We reasoned that mice overexpressing catalase in mitochondria of osteoblast and osteoclast lineage cells would be protected from the bone loss caused by simulated spaceflight. Over-expression of human catalase localized to mitochondria caused various skeletal phenotypic changes compared to WT mice; this includes greater bone length, decreased cortical bone area and moment of inertia, and indications of altered microarchitecture. These findings indicate mitochondrial ROS are important for normal bone-remodeling and skeletal integrity. Catalase over-expression did not fully protect skeletal tissue from structural decrements caused by simulated spaceflight; however there was significant protection in terms of cellular oxidative damage (MDA levels) to the skeletal tissue. Furthermore, we

  12. Identifying and quantifying radiation damage at the atomic level

    PubMed Central

    Gerstel, Markus; Deane, Charlotte M.; Garman, Elspeth F.

    2015-01-01

    Radiation damage impedes macromolecular diffraction experiments. Alongside the well known effects of global radiation damage, site-specific radiation damage affects data quality and the veracity of biological conclusions on protein mechanism and function. Site-specific radiation damage follows a relatively predetermined pattern, in that different structural motifs are affected at different dose regimes: in metal-free proteins, disulfide bonds tend to break first followed by the decarboxylation of aspartic and glutamic acids. Even within these damage motifs the decay does not progress uniformly at equal rates. Within the same protein, radiation-induced electron density decay of a particular chemical group is faster than for the same group elsewhere in the protein: an effect known as preferential specific damage. Here, B Damage, a new atomic metric, is defined and validated to recognize protein regions susceptible to specific damage and to quantify the damage at these sites. By applying B Damage to a large set of known protein structures in a statistical survey, correlations between the rates of damage and various physicochemical parameters were identified. Results indicate that specific radiation damage is independent of secondary protein structure. Different disulfide bond groups (spiral, hook, and staple) show dissimilar radiation damage susceptibility. There is a consistent positive correlation between specific damage and solvent accessibility. PMID:25723922

  13. Genetic damage in subjects exposed to radiofrequency radiation.

    PubMed

    Verschaeve, Luc

    2009-01-01

    Despite many research efforts and public debate there is still great concern about the possible adverse effects of radiofrequency (RF) radiation on human health. This is especially due to the enormous increase of wireless mobile telephones and other telecommunication devices throughout the world. The possible genetic effects of mobile phone radiation and other sources of radiofrequencies constitute one of the major points of concern. In the past several review papers were published on laboratory investigations that were devoted to in vitro and in vivo animal (cyto)genetic studies. However, it may be assumed that some of the most important observations are those obtained from studies with individuals that were exposed to relatively high levels of radiofrequency radiation, either as a result of their occupational activity or as frequent users of radiofrequency emitting tools. In this paper the cytogenetic biomonitoring studies of RF-exposed humans are reviewed. A majority of these studies do show that RF-exposed individuals have increased frequencies of genetic damage (e.g., chromosomal aberrations) in their lymphocytes or exfoliated buccal cells. However, most of the studies, if not all, have a number of shortcomings that actually prevents any firm conclusion. Radiation dosimetry was lacking in all papers, but some of the investigations were flawed by much more severe imperfections. Large well-coordinated multidisciplinary investigations are needed in order to reach any robust conclusion. PMID:19073278

  14. Radiation Damage Effects in Far Ultraviolet Filters and Substrates

    NASA Technical Reports Server (NTRS)

    Keffer, Charles E.; Torr, Marsha R.; Zukic, Muamer; Spann, James F.; Torr, Douglas G.; Kim, Jongmin

    1993-01-01

    New advances in VUV thin film filter technology have been made using filter designs with multilayers of materials such as Al2O3, BaF2, CaF2, HfO2, LaF3, MgF2, and SiO2. Our immediate application for these filters will be in an imaging system to be flown on a satellite where a 2 X 9 R(sub E) orbit will expose the instrument to approximately 275 krads of radiation. In view of the fact that no previous studies have been made on potential radiation damage of these materials in the thin film format, we report on such an assessment here. Transmittances and reflectances of BaF2, CaF2, HfO2, LaF3, MgF2, and SiO2 thin films on MgF2 substrates, Al2O3 thin films on fused silica substrates, uncoated fused silica and MgF2, and four multilayer filters made from these materials were measured from 120 nm to 180 nm before and after irradiation by 250 krads from a Co-60 gamma radiation source. No radiation-induced losses in transmittance or reflectance occurred in this wavelength range. Additional postradiation measurements from 160 nm to 300 nm indicated a 3 - 5% radiation-induced absorption near 260 nm in some of the samples with MgF2 substrates. From these measurements it is concluded that far ultraviolet filters made from the materials tested should experience less that 5% change from exposure to up to 250 krads of high energy radiation in space applications.

  15. [The effect of helium-neon laser radiation on the energy metabolic indices of the myocardium].

    PubMed

    Chizhov, G K; Koval'skaia, N I; Kozlov, V I

    1991-03-01

    It was shown in experiments on white rats, that intravenous and direct myocardium helium-neon laser irradiation leads to the some activation of lactate, glucose-6-phosphate, succinate and reduced NAD degydrogenases. During direct myocardium irradiation these changes are in a less degree. It is suggested that helium-neon laser irradiation displays some active influence on the energy metabolism enzymes of the myocardium, and the mechanisms of this action are discussed. PMID:2054512

  16. Grad-Level Radiation Damage of SIO2 Detectors

    SciTech Connect

    Simos, N.; Atoian, G.; Ludewig, H; White, S; O'Conor, J; Mokhov, N.V.

    2009-05-04

    Radiation effects and levels to detectors. SiO{sub 2} quartz fibers of the LHC ATLAS Zero-degree Calorimeter (ZDC) anticipated to experience integrated doses of a few Grad at their closest position were exposed to 200 MeV protons and neutrons at the Brookhaven National Laboratory (BNL) Linac. Specifically, 1 mm- and 2mm-diameter quartz (GE 124) rods were exposed to direct 200 MeV protons during the first phase of exposure leading to peak integrated dose of {approx}28 Grad. Exposure to a primarily neutron flux of 1mm-diameter SiO{sub 2} fibers was also achieved with a special neutron source arrangement. In a post-irradiation analysis the quartz fiber transmittance was evaluated as a function of the absorbed dose. Dramatic degradation of the transmittance property was observed with increased radiation damage. In addition, detailed evaluation of the fibers under the microscope revealed interesting micro-structural damage features and irradiation-induced defects.

  17. 3D radiative transfer simulations of Eta Carinae's inner colliding winds - II. Ionization structure of helium at periastron

    NASA Astrophysics Data System (ADS)

    Clementel, N.; Madura, T. I.; Kruip, C. J. H.; Paardekooper, J.-P.

    2015-06-01

    Spectral observations of the massive colliding wind binary Eta Carinae show phase-dependent variations, in intensity and velocity, of numerous helium emission and absorption lines throughout the entire 5.54-yr orbit. Approaching periastron, the 3D structure of the wind-wind interaction region (WWIR) gets highly distorted due to the eccentric (e ˜ 0.9) binary orbit. The secondary star (ηB) at these phases is located deep within the primary's dense wind photosphere. The combination of these effects is thought to be the cause of the particularly interesting features observed in the helium lines at periastron. We perform 3D radiative transfer simulations of η Car's interacting winds at periastron. Using the SIMPLEX radiative transfer algorithm, we post-process output from 3D smoothed particle hydrodynamic simulations of the inner 150 au of the η Car system for two different primary star mass-loss rates (dot{M}_{η A}). Using previous results from simulations at apastron as a guide for the initial conditions, we compute 3D helium ionization maps. We find that, for higher dot{M}_{η A}, ηB He0+-ionizing photons are not able to penetrate into the pre-shock primary wind. He+ due to ηB is only present in a thin layer along the leading arm of the WWIR and in a small region close to the stars. Lowering dot{M}_{η A} allows ηB's ionizing photons to reach the expanding unshocked secondary wind on the apastron side of the system, and create a low fraction of He+ in the pre-shock primary wind. With apastron on our side of the system, our results are qualitatively consistent with the observed variations in strength and radial velocity of η Car's helium emission and absorption lines, which helps better constrain the regions where these lines arise.

  18. Imperfection and radiation damage in protein crystals studied with coherent radiation

    PubMed Central

    Nave, Colin; Sutton, Geoff; Evans, Gwyndaf; Owen, Robin; Rau, Christoph; Robinson, Ian; Stuart, David Ian

    2016-01-01

    Fringes and speckles occur within diffraction spots when a crystal is illuminated with coherent radiation during X-ray diffraction. The additional information in these features provides insight into the imperfections in the crystal at the sub-micrometre scale. In addition, these features can provide more accurate intensity measurements (e.g. by model-based profile fitting), detwinning (by distinguishing the various components), phasing (by exploiting sampling of the molecular transform) and refinement (by distinguishing regions with different unit-cell parameters). In order to exploit these potential benefits, the features due to coherent diffraction have to be recorded and any change due to radiation damage properly modelled. Initial results from recording coherent diffraction at cryotemperatures from polyhedrin crystals of approximately 2 µm in size are described. These measurements allowed information about the type of crystal imperfections to be obtained at the sub-micrometre level, together with the changes due to radiation damage. PMID:26698068

  19. Positron annihilation lifetime study of radiation-damaged natural zircons

    NASA Astrophysics Data System (ADS)

    Roberts, J.; Gaugliardo, P.; Farnan, I.; Zhang, M.; Vance, E. R.; Davis, J.; Karatchevtseva, I.; Knott, R. B.; Mudie, S.; Buckman, S. J.; Sullivan, J. P.

    2016-04-01

    Zircons are a well-known candidate waste form for actinides and their radiation damage behaviour has been widely studied by a range of techniques. In this study, well-characterised natural single crystal zircons have been studied using Positron Annihilation Lifetime Spectroscopy (PALS). In some, but not all, of the crystals that had incurred at least half of the alpha-event damage of ∼1019 α/g required to render them structurally amorphous, PALS spectra displayed long lifetimes corresponding to voids of ∼0.5 nm in diameter. The long lifetimes corresponded to expectations from published Small-Angle X-ray Scattering data on similar samples. However, the non-observation by PALS of such voids in some of the heavily damaged samples may reflect large size variations among the voids such that no singular size can be distinguished or. Characterisation of a range of samples was also performed using scanning electron microscopy, optical absorption spectroscopy, Raman scattering and X-ray scattering/diffraction, with the degree of alpha damage being inferred mainly from the Raman technique and X-ray diffraction. The observed void diameters and intensities of the long lifetime components were changed somewhat by annealing at 700 °C; annealing at 1200 °C removed the voids entirely. The voids themselves may derive from He gas bubbles or voids created by the inclusion of small quantities of organic and hydrous matter, notwithstanding the observation that no voidage was evidenced by PALS in two samples containing hydrous and organic matter.

  20. Use of Displacement Damage Dose in an Engineering Model of GaAs Solar Cell Radiation Damage

    NASA Technical Reports Server (NTRS)

    Morton, T. L.; Chock, R.; Long, K. J.; Bailey, S.; Messenger, S. R.; Walters, R. J.; Summers, G. P.

    2005-01-01

    Current methods for calculating damage to solar cells are well documented in the GaAs Solar Cell Radiation Handbook (JPL 96-9). An alternative, the displacement damage dose (D(sub d)) method, has been developed by Summers, et al. This method is currently being implemented in the SAVANT computer program.

  1. Weak-localization effect in superconductors from radiation damage

    NASA Astrophysics Data System (ADS)

    Park, Mi-Ae; Kim, Yong-Jihn

    2000-06-01

    Large reductions of the superconducting transition temperature Tc and the accompanying loss of the thermal electrical resistivity (electron-phonon interaction) due to radiation damage have been observed for several A15 compounds, Chevrel phase and ternary superconductors, and NbSe2 in the high-fluence regime. We examine these behaviors based on a recent theory of the weak localization effect in superconductors. We find a good fitting to the experimental data. In particular, the weak localization correction to the phonon-mediated interaction is derived from the density correlation function. It is shown that weak localization has a strong influence on both the phonon-mediated interaction and the electron-phonon interaction, which leads to the universal correlation of Tc and the resistance ratio.

  2. Chromatin Compaction Protects Genomic DNA from Radiation Damage

    PubMed Central

    Takata, Hideaki; Hanafusa, Tomo; Mori, Toshiaki; Shimura, Mari; Iida, Yutaka; Ishikawa, Kenichi; Yoshikawa, Kenichi; Yoshikawa, Yuko; Maeshima, Kazuhiro

    2013-01-01

    Genomic DNA is organized three-dimensionally in the nucleus, and is thought to form compact chromatin domains. Although chromatin compaction is known to be essential for mitosis, whether it confers other advantages, particularly in interphase cells, remains unknown. Here, we report that chromatin compaction protects genomic DNA from radiation damage. Using a newly developed solid-phase system, we found that the frequency of double-strand breaks (DSBs) in compact chromatin after ionizing irradiation was 5–50-fold lower than in decondensed chromatin. Since radical scavengers inhibited DSB induction in decondensed chromatin, condensed chromatin had a lower level of reactive radical generation after ionizing irradiation. We also found that chromatin compaction protects DNA from attack by chemical agents. Our findings suggest that genomic DNA compaction plays an important role in maintaining genomic integrity. PMID:24130727

  3. Radiation Damage on Multiple Length Scales in Uranium Dioxide

    NASA Astrophysics Data System (ADS)

    Gupta, Mahima

    Radiation damage in UO2 has been well studied but there exists little correlation between point defect accumulation, lattice structure changes and microstructure. This is partly because irradiated nuclear fuel is highly radioactive and its defect chemistry is extremely complicated resulting from fission of the material and consequent fission products being embedded in the fuel matrix [Olander1976]. To adequately study the evolution of defects from point defects through to microstructure features, the resulting defects have to be intentionally simplified for characterization. Ion accelerators have the unique capability of creating simple microstructure features using specific ions, without the added complication of fission and neutron activation from nuclear reactors. As an example, H+ ions have been used to create (only) a distribution of dislocations that were studied using various techniques. The ability to tune the energy or type of the ion to achieve desirable implantation depth and ideally simple microstructure renders it a lucrative instrument for this type of analysis. X-ray diffraction (XRD) studies and transmission electron microscopy (TEM) have been utilized to study extended structure changes and microstructure evolution. Ion beam irradiations create displacements and displacement networks, voids, surface fracturing, gas bubbles and several other microstructure changes to model nuclear reactor damage [Noris1972]. Using an ion accelerator, it has been possible to isolate these radiation induced defects and study their subsequent evolution with increasing dose. Insofar, since all of the phenomena caused by radiation damage originate from point defects, the elucidation of radiation effects on the atomic scale is crucial. This is rendered complicated due to aperiodic irradiation defects. This lack of periodicity renders standard approaches, such as TEM and XRD ineffective, as these methods probe average structure over tens of Angstroms. Therefore, techniques

  4. Development of resistant materials to beam impact and radiation damage

    NASA Astrophysics Data System (ADS)

    Kawai, Masayoshi; Kokawa, Hiroyuki; Okamura, Hiroshi; Kawasaki, Akira; Yamamura, Tsutomu; Hara, Nobuyoshi; Akao, Noboru; Futakawa, Masatoshi; Kikuchi, Kenji

    2006-09-01

    Materials that have strong resistance to both beam impact (or shock-wave) and radiation damage are required for the beam target of an intense accelerator and space applications. Recently, Futakawa et al. found in their experiments that Kolsterising specimens have a stronger resistance to pitting than SS316 CW. A similar effect can be expected for other hardening treatments, and new material development is hopeful. Accordingly, we have started the development of high-performance materials by organizing the project team from KEK, JAEA and universities. In this paper, the scope of the project is introduced. Recent topics involve the development of intergranular crack (IGC)-resistant austenitic stainless-steel, AlN-TiN ceramics and cladding techniques of thin tantalum or CrN film on a tungsten target by means of a molten-salt method and ion-beam-enhanced deposition. New observations on corrosion resistance are presented.

  5. Proton radiation damage in bulk n-GaAs

    NASA Technical Reports Server (NTRS)

    Liu, D. C.; Blue, J. W.; Flood, D. J.; Stanchina, W. E.

    1980-01-01

    Bulk samples of Te-doped n-type GaAs were irradiated using 10 MeV to 24 MeV protons to fluences between 2 x 10 to the 11th power protons/sq cm and 2 x 10 to the 14th power protons/sq cm. Majority carrier electrical effects were measured using the vanderPauw techniques and it was observed that radiation damage was minimal at the 10 to the 11th power proton/sq cm fluence. For the higher fluences, carrier removal was proportional to Delta E/Delta x for the protons indicating ionization interactions between the protons and atoms. Thermal annealing was observed at 155 C.

  6. Dislocation dynamics modelling of radiation damage in thin films

    NASA Astrophysics Data System (ADS)

    Ferroni, Francesco; Tarleton, Edmund; Fitzgerald, Steven

    2014-06-01

    Transmission electron microscopy is a key tool for the extraction of information on radiation damage, the understanding of which is critical for materials development for nuclear fusion and fission reactors. Dislocations in TEM samples are subject to strong image forces, owing to the nanometric sample thicknesses, which may introduce artifacts in the damage analysis. Using dislocation dynamics, we elucidate the roles played by dislocation-surface interactions, dislocation-dislocation interactions and self-interactions due to climb for loop types observed in TEM. Comparisons with analytic solutions for a dislocation loop and an edge dislocation in a half-space are included, and the relationship between glide force and loop tilt examined. The parameters for convergence of the zero-traction boundary conditions are obtained, after which the evolution of dislocation structures in a thin film is studied. It is found that three main length scales govern the physical processes: the image force is governed by the distance of the loop from the surface and scales with the film thickness; the glide force is governed by the image stress as well as the loop-loop interaction stress which is in turn governed by the loop spacing L\\sim 1/\\sqrt{\\rho} , where ρ is the loop density; finally, the climb force depends on the loop size. The three forces compete and their relative magnitudes define the evolution pathway of the dislocation structure.

  7. Influence of radiation damage on krypton diffusion in silicon carbide

    NASA Astrophysics Data System (ADS)

    Friedland, E.; Hlatshwayo, T. T.; van der Berg, N. G.; Mabena, M. C.

    2015-07-01

    Diffusion of krypton in poly and single crystalline silicon carbide is investigated and compared with the previously obtained results for xenon, which pointed to a different diffusion mechanism than observed for chemically active elements. For this purpose 360 keV krypton ions were implanted in commercial 6H-SiC and CVD-SiC wafers at room temperature, 350 °C and 600 °C. Width broadening of the implantation profiles and krypton retention during isochronal and isothermal annealing up to temperatures of 1400 °C was determined by RBS-analysis, whilst in the case of 6H-SiC damage profiles were simultaneously obtained by α-particle channeling. Little diffusion and no krypton loss was detected in the initially amorphized and eventually recrystallized surface layer of cold implanted 6H-SiC during annealing up to 1200 °C. Above that temperature thermal etching of the implanted surface became increasingly important. No diffusion or krypton loss is detected in the hot implanted 6H-SiC samples during annealing up to 1400 °C. Radiation damage dependent grain boundary diffusion is observed at 1300 °C in CVD-SiC. The results seem to indicate, that the chemically inert noble gas atoms do not form defect-impurity complexes, which strongly influence the diffusion behavior of other diffusors in silicon carbide.

  8. Effect of electron excitation on radiation damage in fce metals

    NASA Astrophysics Data System (ADS)

    Iwase, A.; Iwata, T.

    1994-05-01

    Defect production, radiation annealing and defect recovery are studied in several fcc metals (Al, Cu, Ni, Ag and Pt) irradiated with low-energy (˜ 1 MeV) and high-energy (˜ 100 MeV) ions. Irradiation of the metals with strong electron-lattice interaction (Al, Ni and Pt) by ˜ 100 MeV ions causes an anomalous reduction, or even a complete disappearance of stage-I recovery. This experimental result shows that the energy transferred from excited electrons to lattice atoms through the electron-lattice interaction contributes to the annihilation of single interstitials. This effect is also observed in Ni as a large cross section for radiation annealing, and a decrease of the damage efficiency. On the other hand, in Cu and Ag thin foils, we find that lattice defects are produced not only through elastic interactions, but also through a process strongly associated with electron excitation. In the latter process, the defect production cross section is proportional to Se1.7 in Cu and Se1.5 in Ag. The nearly quadratic dependence of the cross section on Se suggests that the mutual Coulomb repulsion of ions positively charged by electron excitation causes the defect production.

  9. Acute radiation-induced pulmonary damage: a clinical study on the response to fractionated radiation therapy.

    PubMed

    Mah, K; Van Dyk, J; Keane, T; Poon, P Y

    1987-02-01

    Acute radiation-induced pulmonary damage can be a significant cause of morbidity in radiation therapy of the thorax. A prospective, clinical study was conducted to obtain dose-response data on acute pulmonary damage caused by fractionated radiation therapy. The endpoint was a visible increase in lung density within the irradiated volume on a computed tomographic (CT) examination as observed independently by three diagnostic radiologists. Fifty-four patients with various malignancies of the thorax completed the study. CT chest scans were taken before and at preselected times following radiotherapy. To represent different fractionation schedules of equivalent biological effect, the estimated single dose (ED) model, ED = D X N-0.377 X T-0.058 was used in which D was the average lung dose within the high dose region in cGy, N was the number of fractions, and T was the overall treatment time in days. Patients were grouped according to ED and the percent incidence of pulmonary damage for each group was determined. Total average lung doses ranged from 29.8 Gy to 53.6 Gy given in 10 to 30 fractions over a range of 12 to 60 days. Five patient groups with incidence ranging from 30% (ED of 930) to 90% (ED of 1150) were obtained. The resulting dose-response curve predicted a 50% incidence level at an ED value (ED50) of 1000 +/- 40 ED units. This value represents fractionation schedules equivalent to a total average lung dose of 32.9 Gy given in 15 fractions over 19 days. Over the linear portion of the dose-response curve, a 5% increase in ED (or total dose if N and T remain constant), predicts a 12% increase in the incidence of acute radiation-induced pulmonary damage. PMID:3818385

  10. INVESTIGATION OF MICROSCOPIC RADIATION DAMAGE IN WASTE FORMS USING ODNMR AND AEM TECHNIQUES

    EPA Science Inventory

    The proposed project addresses DOE nuclear waste problems that are currently intractable without achieving a fundamental understanding of radiation effects on high level waste (HLW) forms. This project will investigate the microscopic effects of radiation damage in crystalline an...

  11. Transmission electron microscope study of fusion-environment radiation damage in iron and iron-chromium alloys

    SciTech Connect

    Horton, L.L.S.

    1982-07-01

    A transmission electron microscopy study of radiation damage microstructures in iron and iron-chromium alloys has been performed. This study consisted of both qualitative and quantitative characterization of the dislocation and cavity microstructures, including determination of vacancy/interstitial character and Burgers vectors for dislocation loops and analysis of the cavity morphology. The effects of irradiation temperature, fluence, helium implantation, and chromium content were investigated. Neutron irradiation (iron specimens, 1 dpa, 455 to 1000 K) and triple-beam ion irradiation (Fe-10% Cr specimens, 10 dpa, 725 to 950 K; Fe-10% Cr specimens, 850 K, 0.3 to 100 dpa; and Fe, Fe-5% Cr, Fe-10% Cr specimens, 850 K, 10 dpa) were employed. In the triple-beam ion irradiation procedure, simultaneous bombardment with 4 MeV Fe/sup + +/ ions and energetic He/sup +/ and D/sub 2//sup +/ ions was used to simulate the fusion environment (10 at. ppM He/dpa and 41 at. ppM D/dpa). In addition, single-beam 4 MeV Fe/sup + +/ ion irradiations of Fe-10% Cr both with and without pre-injection of helium and deuterium were performed.

  12. A FLUORESCENCE BASED ASSAY FOR DNA DAMAGE: INDUCED BY RADIATION, CHEMICALS AND ENZYMES

    EPA Science Inventory

    A simple and rapid assay to detect DNA damage is reported. This assay is based on the ability of certain dyes to fluoresce upon intercalation with dsDNA. Damage caused by ultraviolet (UV) radiation, chemicals or restriction enzymes is detected using this assay. UV radiation at...

  13. Potential vascular damage from radiation in the space environment

    NASA Astrophysics Data System (ADS)

    Griem, M. L.; Robotewskyj, A.; Nagel, R. H.

    1994-10-01

    Cultured endothelial cells of blood vessels have a Do of 2 Gy for X-rays. A dose of 0.5 Gy of X-rays has an acute effect on vessel diameter. The vessels may show other acute effects such as change in permeability including a change in the blood brain barrier. Changes occurring from late effects of chronic exposure in vascular architecture include telangiectasia and decrease in vascular density. Changes in the perivascular connective tissue particularly collagen may play a role in these changes. After charged particle exposure of 15 and 30 Gy, radiation changes in the blood brain barrier and vascular changes are noted in the nervous system. These long term changes are recorded by PET, MRI, and CT imaging. Chronic exposure to alpha particles causes vascular damage in compact bone resulting in bone infarcts. Using tandem scanning confocal microscopy in-situ imaging of the capillaries and collagen of the papillary dermis provides a non-invasive method of serial recording of changes in irradiated microvasculature.

  14. UV radiation and freshwater zooplankton: damage, protection and recovery

    PubMed Central

    Rautio, Milla; Tartarotti, Barbara

    2011-01-01

    While many laboratory and field studies show that zooplankton are negatively affected when exposed to high intensities of ultraviolet radiation (UVR), most studies also indicate that zooplankton are well adapted to cope with large variations in their UVR exposure in the pelagic zone of lakes. The response mechanisms of zooplankton are diverse and efficient and may explain the success and richness of freshwater zooplankton in optically variable waters. While no single behavioural or physiological protection mechanism seems to be superior, and while several unexplained and contradictory patterns exist in zooplankton UVR ecology, recent increases in our understanding are consistent with UVR playing an important role for zooplankton. This review examines the variability in freshwater zooplankton responses to UVR, with a focus on crustacean zooplankton (Cladocera and Copepoda). We present an overview of UVR-induced damages, and the protection and recovery mechanisms freshwater zooplankton use when exposed to UVR. We review the current knowledge of UVR impact on freshwater zooplankton at species and community levels, and discuss briefly how global change over the last three decades has influenced the UVR milieu in lakes. PMID:21516254

  15. Radiation damage of BGO and CsI(Tl) crystals

    NASA Astrophysics Data System (ADS)

    Bieler, Ch.; Burkart, D.; Marks, J.; Riebesell, M.; Spitzer, H.; Wittenburg, K.; Winter, G.-G.

    1985-02-01

    We have measured the response of five 10-20 cm long BGO crystals from different manufacturers to irradiation with 137Cs γ-rays at doses of 40 and 85 rad. Immediately after irradiation the pulse height drops by 26-38% and recovers only partially with time. 110 d after irradiation the remaining damage is between 1 and 13%. A 10 cm long CsI (Tl) crystal shows pulse height reductions after irradiation which do not recover with time. The cumulative effect of a continuous irradiation on a BGO crystal (1 × 1× 15 cm 3) in a partially shielded position on the beam pipe of PETRA was measured over a period of 53 d. At an average daily dose of 1.9 rad the pulse height dropped continuously resulting in an overall pulse height loss of 9% in 7 weeks. This indicates that BGO when applied in long and narrow shapes is more sensitive to small radiation doses than previously assumed.

  16. Primary radiation damage of protein crystals by an intense synchrotron X-ray beam.

    PubMed

    Teng, T Y; Moffat, K

    2000-09-01

    X-ray radiation damage of a lysozyme single crystal by an intense monochromatic beam from a third-generation radiation source at the Advanced Photon Source has been studied. The results show that primary radiation damage is linearly dependent on the X-ray dose even when the crystal is at cryogenic temperatures. The existence of an upper limit for the primary radiation damage was observed. Above the threshold of approximately 1 x 10(7) Gy, excessive damage of the crystal develops which is interpreted as the onset of secondary and/or tertiary radiation damage. This upper limit of X-ray dose is compared with Henderson's limit [Henderson (1990). Proc. R. Soc. London, B241, 6-8], and its implication for the amount of useful X-ray diffraction data that can be obtained for crystals of a given scattering power is also discussed. PMID:16609214

  17. Clustered DNA damages induced in human hematopoietic cells by low doses of ionizing radiation

    NASA Technical Reports Server (NTRS)

    Sutherland, Betsy M.; Bennett, Paula V.; Cintron-Torres, Nela; Hada, Megumi; Trunk, John; Monteleone, Denise; Sutherland, John C.; Laval, Jacques; Stanislaus, Marisha; Gewirtz, Alan

    2002-01-01

    Ionizing radiation induces clusters of DNA damages--oxidized bases, abasic sites and strand breaks--on opposing strands within a few helical turns. Such damages have been postulated to be difficult to repair, as are double strand breaks (one type of cluster). We have shown that low doses of low and high linear energy transfer (LET) radiation induce such damage clusters in human cells. In human cells, DSB are about 30% of the total of complex damages, and the levels of DSBs and oxidized pyrimidine clusters are similar. The dose responses for cluster induction in cells can be described by a linear relationship, implying that even low doses of ionizing radiation can produce clustered damages. Studies are in progress to determine whether clusters can be produced by mechanisms other than ionizing radiation, as well as the levels of various cluster types formed by low and high LET radiation.

  18. Effect of boundary conditions on the kinetics of helium release from structural materials

    NASA Astrophysics Data System (ADS)

    Zaluzhnyi, A. G.

    2015-11-01

    Gaseous products of nuclear reactions (specifically, helium) play a significant part in altering the material properties upon irradiation. It is known that atoms of inert gases promote the generation and growth of pores in irradiated materials and affect phenomena such as swelling, high-temperature irradiation embrittlement, etc. Therefore, a study of the behavior of helium (its production, accumulation, retention, and release) within structural materials is fairly topical. In order to validate the methods of express imitation of accumulation and retention of helium within structural materials under reactor irradiation, we perform a comparative analysis of the spectra of the rate of gas release from samples of austenitic steel 0Kh16N15M3B that were saturated with helium in different ways, i.e., through irradiation in a cyclotron, a magnetic massseparation setup, the IRT-2000 reactor, the BOR-60 reactor, and using the so-called tritium trick technique. The effect of the presence of dislocations and grain boundaries on the release of helium from materials is evaluated. The results of the research conducted show that the kinetics of helium release from samples saturated with helium through the bombardment with alpha particles of different energies, which ensures the simultaneous introduction of helium and radiation defects (in wide ranges of helium concentration and radiation damage) into the material lattice, is similar to the kinetics of helium release from samples irradiated in reactors.

  19. In situ radiation test of silicon and diamond detectors operating in superfluid helium and developed for beam loss monitoring

    NASA Astrophysics Data System (ADS)

    Kurfürst, C.; Dehning, B.; Sapinski, M.; Bartosik, M. R.; Eisel, T.; Fabjan, C.; Rementeria, C. A.; Griesmayer, E.; Eremin, V.; Verbitskaya, E.; Zabrodskii, A.; Fadeeva, N.; Tuboltsev, Y.; Eremin, I.; Egorov, N.; Härkönen, J.; Luukka, P.; Tuominen, E.

    2015-05-01

    As a result of the foreseen increase in the luminosity of the Large Hadron Collider, the discrimination between the collision products and possible magnet quench-provoking beam losses of the primary proton beams is becoming more critical for safe accelerator operation. We report the results of ongoing research efforts targeting the upgrading of the monitoring system by exploiting Beam Loss Monitor detectors based on semiconductors located as close as possible to the superconducting coils of the triplet magnets. In practice, this means that the detectors will have to be immersed in superfluid helium inside the cold mass and operate at 1.9 K. Additionally, the monitoring system is expected to survive 20 years of LHC operation, resulting in an estimated radiation fluence of 1×1016 proton/cm2, which corresponds to a dose of about 2 MGy. In this study, we monitored the signal degradation during the in situ irradiation when silicon and single-crystal diamond detectors were situated in the liquid/superfluid helium and the dependences of the collected charge on fluence and bias voltage were obtained. It is shown that diamond and silicon detectors can operate at 1.9 K after 1×1016 p/cm2 irradiation required for application as BLMs, while the rate of the signal degradation was larger in silicon detectors than in the diamond ones. For Si detectors this rate was controlled mainly by the operational mode, being larger at forward bias voltage.

  20. Micro-inhomogeneity effects and radiation damage in semi-insulating GaAs radiation detectors

    SciTech Connect

    Bates, R.; O`Shea, V.; Raine, C.; Smith, K.M.; Didziulis, R.; Kazukauskas, V.; Rinkevicius, V.; Storasta, J.; Vaitkus, J.

    1998-06-01

    Thermally-stimulated current (TSC) measurements and a detailed analysis of current-voltage (I-V) characteristics have been made on semi-insulating GaAs (SI-GaAs) Schottky diode particle detectors, fabricated on substrates from several supplies, before and after irradiation with 24 GeV protons and 300 MeV pions. The analysis of I-V characteristics allows the determination of the barrier height and bulk resistance in detectors. Changes observed in I-V characteristics and TSC spectra after irradiation are described and a dislocation-net model of radiation-damaged devices is proposed.

  1. Research progress in radiation detectors, pattern recognition programs, and radiation damage determination in DNA

    NASA Technical Reports Server (NTRS)

    Baily, N. A.

    1973-01-01

    The radiological implications of statistical variations in energy deposition by ionizing radiation were investigated in the conduct of the following experiments: (1) study of the production of secondary particles generated by the passage of the primary radiation through bone and muscle; (2) the study of the ratio of nonreparable to reparable damage in DNA as a function of different energy deposition patterns generated by X rays versus heavy fast charged particles; (3) the use of electronic radiography systems for direct fluoroscopic tomography and for the synthesis of multiple planes and; (4) the determination of the characteristics of systems response to split fields having different contrast levels, and of minimum detectable contrast levels between the halves under realistic clinical situations.

  2. Radiation damage studies related to nuclear waste forms

    SciTech Connect

    Gray, W.J.; Wald, J.W.; Turcotte, R.P.

    1981-12-01

    Much of the previously reported work on alpha radiation effects on crystalline phases of importance to nuclear waste forms has been derived from radiation effects studies of composite waste forms. In the present work, two single-phase crystalline materials, Gd/sub 2/Ti/sub 2/O/sub 7/ (pyrochlore) and CaZrTi/sub 2/O/sub 7/ (zirconolite), of relative importance to current waste forms were studied independently by doping with /sup 244/Cm at the 3 wt % level. Changes in the crystalline structure measured by x-ray diffraction as a function of dose show that damage ingrowth follows an expected exponential relationship of the form ..delta..V/V/sub 0/ = A(1-exp(-BD)). In both cases, the materials became x-ray amorphous before the estimated saturation value was reached. The predicted magnitudes of the unit cell volume changes at saturation are 5.4% and 3.5%, respectively, for Gd/sub 2/Ti/sub 2/O/sub 7/ and CaZrTi/sub 2/O/sub 7/. The later material exhibited anisotropic behavior in which the expansion of the monoclinic cell in the c/sub 0/ direction was over five times that of the a/sub 0/ direction. The effects of transmutations on the properties of high-level waste solids have not been studied until now because of the long half-lives of the important fission products. This problem was circumvented in the present study by preparing materials containing natural cesium and then irradiating them with neutrons to produce /sup 134/Cs, which has only a 2y half-life. The properties monitored at about one year intervals following irradiation have been density, leach rate and microstructure. A small amount of x-ray diffraction work has also been done. Small changes in density and leach rate have been observed for some of the materials, but they were not large enough to be of any consequence for the final disposal of high level wastes.

  3. Recent radiation damage studies and developments of the Marlowe code

    NASA Astrophysics Data System (ADS)

    Ortiz, C. J.; Souidi, A.; Becquart, C. S.; Domain, C.; Hou, M.

    2014-07-01

    Radiation damage in materials relevant to applications evolves over time scales spanning from the femtosecond - the characteristic time for an atomic collision - to decades - the aging time expected for nuclear materials. The relevant kinetic energies of atoms span from thermal motion to the MeV range.The question motivating this contribution is to identify the relationship between elementary atomic displacements triggered by irradiation and the subsequent microstructural evolution of metals in the long term. The Marlowe code, based on the binary collision approximation (BCA) is used to simulate the sequences of atomic displacements generated by energetic primary recoils and the Object Kinetic Monte Carlo code LAKIMOCA, parameterized on a range of ab initio calculations, is used to predict the subsequent long-term evolution of point defect and clusters thereof. In agreement with full Molecular Dynamics, BCA displacement cascades in body-centered cubic (BCC) Fe and a face-centered cubic (FCC) Febond Nibond Cr alloy display recursive properties that are found useful for predictions in the long term.The case of defects evolution in W due to external irradiation with energetic H and He is also discussed. To this purpose, it was useful to extend the inelastic energy loss model available in Marlowe up to the Bethe regime. The last version of the Marlowe code (version 15) was delivered before message passing instructions softwares (such as MPI) were available but the structure of the code was designed in such a way to permit parallel executions within a distributed memory environment. This makes possible to obtain N different cascades simultaneously using N independent nodes without any communication between processors. The parallelization of the code using MPI was recently achieved by one author of this report (C.J.O.). Typically, the parallelized version of Marlowe allows simulating millions of displacement cascades using a limited number of processors (<64) within only

  4. Molecular responses of radiation-induced liver damage in rats

    PubMed Central

    CHENG, WEI; XIAO, LEI; AINIWAER, AIMUDULA; WANG, YUNLIAN; WU, GE; MAO, RUI; YANG, YING; BAO, YONGXING

    2015-01-01

    The aim of the present study was to investigate the molecular responses involved in radiation-induced liver damage (RILD). Sprague-Dawley rats (6-weeks-old) were irradiated once at a dose of 20 Gy to the right upper quadrant of the abdomen. The rats were then sacrificed 3 days and 1, 2, 4, 8 and 12 weeks after irradiation and rats, which were not exposed to irradiation were used as controls. Weight measurements and blood was obtained from the rats and liver tissues were collected for histological and apoptotic analysis. Immunohistochemistry, reverse transcription quantitative polymerase chain reaction (RT-qPCR) and western blot analysis were performed to measure the expression levels of mRNAs and proteins, respectively. The serum levels of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase were increased significantly in the RILD rats. Histological investigation revealed the proliferation of collagen and the formation of fibrotic tissue 12 weeks after irradiation. Apoptotic cells were observed predominantly 2 and 4 weeks after irradiation. The immunohistochemistry, RT-qPCR and western blot analysis all revealed the same pattern of changes in the expression levels of the molecules assessed. The expression levels of transforming growth factor-β1 (TGF-β1), nuclear factor (NF)-κB65, mothers against decapentaplegic homolog 3 (Smad3) and Smad7 and connective tissue growth factor were increased during the recovery period following irradiation up to 12 weeks. The expression levels of tumor necrosis factor-α, Smad7 and Smad4 were only increased during the early phase (first 4 weeks) of recovery following irradiation. In the RILD rat model, the molecular responses indicated that the TGF-β1/Smads and NF-κB65 signaling pathways are involved in the mechanism of RILD recovery. PMID:25483171

  5. Use of the tritium trick for analysis of the influence that hydrogen and helium exert on the mechanical properties of radiation-resistant precipitation-hardening FCC steels

    SciTech Connect

    Goshchitskii, B.N.; Sagaradze, V.V.; Arbuzov, V.L.; Zuev, Y.N.; Markelov, N.N.; Zimin, A.V.

    1995-10-01

    The aging austenitic stainless steel Crl6Nil5Mo3Til is shown to possess a high resistance to the radiation void formation under irradiation with fast neutrons (60 dpa, 753K). The influence of tritium introduced from the gaseous phase and radiogenic helium on the mechanical properties is analysed. 3 refs., 3 tabs.

  6. Influence of hydrogen isotopes and radiogenic helium on the structure and mechanical properties of radiation-resistant steels with a composite alpha/gamma structure

    SciTech Connect

    Sagaradze, V.V.; Arbuzov, V.L.; Lapin, S.S.; Zuev, Y.N.; Markelov, N.N.; Dolinski, Y.N.

    1995-10-01

    It is shown that the Cr-Ni-Mo stainless steel with a fine-plate austenitic-martensitic structure has a high resistance to radiation swelling. The influence of small amounts of tritium (0.015 at.%) and radiogenic helium (approx.2.5 appm) on the mechanical proper ties of the steel is analysed. 3 refs., 2 figs., 1 tab.

  7. Protection of radiation detectors from fast neutron damage

    SciTech Connect

    Kronenberg, S.

    1986-09-02

    A device is described for measuring radiation emitted from a nuclear explosion, the radiation having a comparatively fast moving gamma ray component and a comparatively slower neutron component. The device consists of: a solid state crystal radiation detector; a voltage source applied to bias the detector; and means responsive to the gamma ray component for removing the bias voltage for a predetermined time period whereby the crystal radiation detector is rendered less sensitive to the passage of the neutron radiation component.

  8. Infrared A radiation promotes survival of human melanocytes carrying ultraviolet radiation-induced DNA damage.

    PubMed

    Kimeswenger, Susanne; Schwarz, Agatha; Födinger, Dagmar; Müller, Susanne; Pehamberger, Hubert; Schwarz, Thomas; Jantschitsch, Christian

    2016-06-01

    The link between solar radiation and melanoma is still elusive. Although infrared radiation (IR) accounts for over 50% of terrestrial solar energy, its influence on human skin is not well explored. There is increasing evidence that IR influences the expression patterns of several molecules independently of heat. A previous in vivo study revealed that pretreatment with IR might promote the development of UVR-induced non-epithelial skin cancer and possibly of melanoma in mice. To expand on this, the aim of the present study was to evaluate the impact of IR on UVR-induced apoptosis and DNA repair in normal human epidermal melanocytes. The balance between these two effects is a key factor of malignant transformation. Human melanocytes were exposed to physiologic doses of IR and UVR. Compared to cells irradiated with UVR only, simultaneous exposure to IR significantly reduced the apoptotic rate. However, IR did not influence the repair of UVR-induced DNA damage. IR partly reversed the pro-apoptotic effects of UVR via modification of the expression and activity of proteins mainly of the extrinsic apoptotic pathway. In conclusion, IR enhances the survival of melanocytes carrying UVR-induced DNA damage and thereby might contribute to melanomagenesis. PMID:26844814

  9. Radiation-Induced Liver Damage: Correlation of Histopathology with Hepatobiliary Magnetic Resonance Imaging, a Feasibility Study

    SciTech Connect

    Seidensticker, Max; Burak, Miroslaw; Kalinski, Thomas; Garlipp, Benjamin; Koelble, Konrad; Wust, Peter; Antweiler, Kai; Seidensticker, Ricarda; Mohnike, Konrad; Pech, Maciej; Ricke, Jens

    2015-02-15

    PurposeRadiotherapy of liver malignancies shows promising results (radioembolization, stereotactic irradiation, interstitial brachytherapy). Regardless of the route of application, a certain amount of nontumorous liver parenchyma will be collaterally damaged by radiation. The functional reserve may be significantly reduced with an impact on further treatment planning. Monitoring of radiation-induced liver damage by imaging is neither established nor validated. We performed an analysis to correlate the histopathological presence of radiation-induced liver damage with functional magnetic resonance imaging (MRI) utilizing hepatobiliary contrast media (Gd-BOPTA).MethodsPatients undergoing local high-dose-rate brachytherapy for whom a follow-up hepatobiliary MRI within 120 days after radiotherapy as well as an evaluable liver biopsy from radiation-exposed liver tissue within 7 days before MRI were retrospectively identified. Planning computed tomography (CT)/dosimetry was merged to the CT-documentation of the liver biopsy and to the MRI. Presence/absence of radiation-induced liver damage (histopathology) and Gd-BOPTA uptake (MRI) as well as the dose applied during brachytherapy at the site of tissue sampling was determined.ResultsFourteen biopsies from eight patients were evaluated. In all cases with histopathological evidence of radiation-induced liver damage (n = 11), no uptake of Gd-BOPTA was seen. In the remaining three, cases no radiation-induced liver damage but Gd-BOPTA uptake was seen. Presence of radiation-induced liver damage and absence of Gd-BOPTA uptake was correlated with a former high-dose exposition.ConclusionsAbsence of hepatobiliary MRI contrast media uptake in radiation-exposed liver parenchyma may indicate radiation-induced liver damage. Confirmatory studies are warranted.

  10. Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

    PubMed Central

    Lomb, Lukas; Barends, Thomas R. M.; Kassemeyer, Stephan; Aquila, Andrew; Epp, Sascha W.; Erk, Benjamin; Foucar, Lutz; Hartmann, Robert; Rudek, Benedikt; Rolles, Daniel; Rudenko, Artem; Shoeman, Robert L.; Andreasson, Jakob; Bajt, Sasa; Barthelmess, Miriam; Barty, Anton; Bogan, Michael J.; Bostedt, Christoph; Bozek, John D.; Caleman, Carl; Coffee, Ryan; Coppola, Nicola; DePonte, Daniel P.; Doak, R. Bruce; Ekeberg, Tomas; Fleckenstein, Holger; Fromme, Petra; Gebhardt, Maike; Graafsma, Heinz; Gumprecht, Lars; Hampton, Christina Y.; Hartmann, Andreas; Hauser, Günter; Hirsemann, Helmut; Holl, Peter; Holton, James M.; Hunter, Mark S.; Kabsch, Wolfgang; Kimmel, Nils; Kirian, Richard A.; Liang, Mengning; Maia, Filipe R. N. C.; Meinhart, Anton; Marchesini, Stefano; Martin, Andrew V.; Nass, Karol; Reich, Christian; Schulz, Joachim; Seibert, M. Marvin; Sierra, Raymond; Soltau, Heike; Spence, John C. H.; Steinbrener, Jan; Stellato, Francesco; Stern, Stephan; Timneanu, Nicusor; Wang, Xiaoyu; Weidenspointner, Georg; Weierstall, Uwe; White, Thomas A.; Wunderer, Cornelia; Chapman, Henry N.; Ullrich, Joachim; Strüder, Lothar; Schlichting, Ilme

    2013-01-01

    X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects. PMID:24089594

  11. Radiation damage in protein crystals is reduced with a micron-sized X-ray beam

    PubMed Central

    Sanishvili, Ruslan; Yoder, Derek W.; Pothineni, Sudhir Babu; Rosenbaum, Gerd; Xu, Shenglan; Vogt, Stefan; Stepanov, Sergey; Makarov, Oleg A.; Corcoran, Stephen; Benn, Richard; Nagarajan, Venugopalan; Smith, Janet L.; Fischetti, Robert F.

    2011-01-01

    Radiation damage is a major limitation in crystallography of biological macromolecules, even for cryocooled samples, and is particularly acute in microdiffraction. For the X-ray energies most commonly used for protein crystallography at synchrotron sources, photoelectrons are the predominant source of radiation damage. If the beam size is small relative to the photoelectron path length, then the photoelectron may escape the beam footprint, resulting in less damage in the illuminated volume. Thus, it may be possible to exploit this phenomenon to reduce radiation-induced damage during data measurement for techniques such as diffraction, spectroscopy, and imaging that use X-rays to probe both crystalline and noncrystalline biological samples. In a systematic and direct experimental demonstration of reduced radiation damage in protein crystals with small beams, damage was measured as a function of micron-sized X-ray beams of decreasing dimensions. The damage rate normalized for dose was reduced by a factor of three from the largest (15.6 μm) to the smallest (0.84 μm) X-ray beam used. Radiation-induced damage to protein crystals was also mapped parallel and perpendicular to the polarization direction of an incident 1-μm X-ray beam. Damage was greatest at the beam center and decreased monotonically to zero at a distance of about 4 μm, establishing the range of photoelectrons. The observed damage is less anisotropic than photoelectron emission probability, consistent with photoelectron trajectory simulations. These experimental results provide the basis for data collection protocols to mitigate with micron-sized X-ray beams the effects of radiation damage. PMID:21444772

  12. Electron energy distribution in a helium plasma created by nuclear radiations

    NASA Technical Reports Server (NTRS)

    Lo, R. H.; Miley, G. H.

    1974-01-01

    An integral balance technique for calculation of the electron energy distribution in a radiation-induced plasma is described. Results predict W-values reasonably well and compare favorably with more complicated Monte-Carlo calculations. The distribution found differs from that in a normal electrical discharge and is of interest in radiation-pumped laser research.

  13. The Radiation environment and damage in the CDF tracking volume

    SciTech Connect

    R. J. Tesarek et al.

    2003-12-16

    The authors present direct measurements of the spatial distribution of ionizing radiation and low energy neutrons (E{sub n} < 200 keV) inside the tracking volume of the collider detector at Fermilab (CDF). Using data from multiple exposures, the radiation field can be separated into components from beam losses and collisions and can be checked for consistency between the measurements. They compare the radiation measurements with an increase in the leakage currents of the CDF silicon detectors and find reasonable agreement.

  14. Radiation damage to tetramethlysilane and tetramethlygermanium ionization chambers

    NASA Astrophysics Data System (ADS)

    Hoshi, Y.; Higuchi, M.; Oyama, K.; Akaishi, H.; Yuta, H.; Abe, K.; Hasegawa, K.; Suekane, F.; Nagamine, T.; Kawamura, N.

    1994-08-01

    Two detector media suitable for a warm liquid, ionization chamber filled with tetramethylsilane(TMS) and tetramethylgermanium(TMG) were exposed to y radiation from a (sup 60)Co source up to dose 579Gray and 902Gray, respectively. The electron lifetimes and the free ion yields were measured as a function of accumulated radiation dose. A similar behavior of the electron lifetimes and the free ion yields with increasing radiation dose was observed between the TMS and TMG ionization chambers.

  15. Annealing radiation damaged silicon solar cells with a copper halide laser

    NASA Technical Reports Server (NTRS)

    Pivirotto, T. J.

    1980-01-01

    The use of a multiply pulsed copper halide laser to significantly anneal out the damage to silicon solar cells caused by a simulated space radiation environment is investigated. Preliminary experiments demonstrate that the amount of damage can be decreased by 41% as measured by the maximum power generated.

  16. The Effects of Interfaces on Radiation Damage Production in Layered Metal Composites

    SciTech Connect

    Heinisch, Howard L.; Gao, Fei; Kurtz, Richard J.

    2003-12-07

    The objective of this research is to use molecular dynamics modeling to explore the effects of interfaces on cascade-producing radiation damage in nanolayered metal composites and to assess the resistance of these composites to damage by neutrons in fusion devices.

  17. Does iodinated contrast medium amplify DNA damage during exposure to radiation.

    PubMed

    Riley, Peter

    2015-01-01

    There is a recognized increased risk of cancer following exposure of humans to ionizing radiation; this is felt to be most likely due to damage to DNA strands during exposure. Damage to DNA strands can be demonstrated microscopically following exposure to X-rays, and new evidence is emerging that this effect may be compounded by administration of iodinated contrast agents. PMID:26234959

  18. Characterization of a compact ECR plasma source and its applications to studies of helium ion damage to tungsten

    NASA Astrophysics Data System (ADS)

    Donovan, D.; Buchenauer, D.; Whaley, J.; Friddle, R.

    2016-02-01

    Exposure of tungsten to low energy (<100 eV) helium plasmas at temperatures between 900-1900 K in both laboratory experiments and tokamaks has been shown to cause severe nanoscale modification of the near surface resulting in the growth of tungsten tendrils. Tendril formation can lead to non-sputtered erosion and dust formation. Here we report on characterization of a compact electron cyclotron resonance (ECR) He plasma source with an ion flux of ˜2.5 × 1019 ions m-2 s-1, average fluence of 3 × 1024 ions m-2, and the surface morphology changes seen on the exposed tungsten surfaces. Exposures of polished tungsten disks at temperatures up to 1270 K have been performed and characterized using scanning electron microscopy and atomic force microscopy (AFM) scans. Bubbles and craters have been seen on the exposed tungsten surface growing to up to 150 nm in diameter. The ECR source has been tested for eventual use on a scanning tunneling microscopy experiment intended to study the early stages of surface morphology change due to He ion exposure.

  19. Helium-neon laser radiation effect on fish embryos and larvae

    NASA Astrophysics Data System (ADS)

    Uzdensky, Anatoly B.

    1994-09-01

    Helium-neon laser irradiation (HNLI) is an effective biostimulating agent but its influence on embryonal processes is almost unknown. We have studied fish embryos and larvae development, viability, and growth after HNLI of fish eggs at different stages. With this aim carp, grass carp, sturgeon, and stellared sturgeon eggs were incubated in Petri plates or in fish-breeding apparatuses and were irradiated in situ with different exposures. Then we studied hutchling percentage, larvae survival and growth dynamics, and morphological anomalies percentage. HNLI effect depended on irradiation exposures and intensity, embryonal stages, and fish species. Laser eggs irradiation essentially affected larvae viability and growth in the postembryonal phase. For example, HNLI of sturgeon spawn at cleavage stage or grass carp at organogenesis decreased larvae survival rate. On the contrary HNLI at gastrulation or embryonal motorics stages markedly increased larvae survival rate and decreased the morphological anomalies percentage. We determined most effective irradiation regimes depending of fish species which may be used in practical fish-breeding.

  20. Evolution of defects in silicon carbide implanted with helium ions

    NASA Astrophysics Data System (ADS)

    Zhang, Chonghong; Song, Yin; Yang, Yitao; Zhou, Chunlan; Wei, Long; Ma, Hongji

    2014-05-01

    Effects of accumulation of radiation damage in silicon carbide are important concerns for the use of silicon carbide in advanced nuclear energy systems. In the present work lattice damage in silicon carbide crystal (4H type) implanted with 100 keV 4He+ ions was investigated with Rutherford backscattering spectrometry in channeling geometry (RBS/c) and positron beam Doppler broadening spectrometry (PBDB). Helium implantation was performed at the specimen temperature of 510 K to avoid amorphization of the SiC crystal. Fluences of helium ions were selected to be in the range from 1 × 1016 to 3 × 1016 ions cm-2, around the dose threshold for the formation of observable helium bubbles under transmission electron microscopes (TEM). The RBS/c measurements show distinctly different annealing behavior of displaced Si atoms at doses below or above the threshold for helium bubble formation. The RBS/c yield in the peak damage region of the specimen implanted to 3 × 1016 He-ions cm-2 shows an increase on the subsequently thermal annealing above 873 K, which is readily ascribed to the extra displacement of Si atoms due to helium bubble growth. The RBS/c yield in the specimen implanted to a lower ion fluence of 1.5 × 1016 He-ions cm-2 decreases monotonously on annealing from ambient temperatures up to 1273 K. The PBDB measurements supply evidence of clustering of vacancies at temperatures from 510 to 1173 K, and dissociation of vacancy clusters above 1273 K. The similarity of annealing behavior in PBDB profiles for helium implantation to 1 × 1016 and 3 × 1016 ions cm-2 is ascribed to the saturation of trapping of positrons in vacancy type defects in the damaged layers in the specimens helium-implanted to the two dose levels.

  1. Calculation of hydrogen and helium concentrations for CSNS target

    NASA Astrophysics Data System (ADS)

    Pan, Dong-Dong; Liang, Tai-Ran; Yin, Wen; Yao, Ze-En

    2016-03-01

    The China Spallation Neutron Source (CSNS) is driven by protons whose energies are about 1.6 GeV. At such high energies, the spallation neutrons lead to the formation of large amounts of helium, hydrogen and new heavier species in the form of transmutation products. These hydrogen, helium and transmutation products have a critical effect on the mechanical properties on the one hand and exacerbate the displacement radiation damage on the other hand. In this paper, the background hydrogen/helium concentrations and the maximum hydrogen/helium concentrations near cracks in a tungsten target for CSNS have been calculated at temperatures of 100°C and 300°C by applying a theoretical model. For the CSNS tungsten target plate, we find the maximum hydrogen concentration near the tips of cracks ranges from 3.0 × 10-2-2 × 10-1, which exceeds the hydrogen background concentration by 1.2-1.8 times; the maximum helium concentration near the tips of cracks ranges from 3.0 × 10-4 -1.2 × 10-3, which exceeds the helium background concentration by 2-4 times; the maximum hydrogen/helium concentration increases with the increase of the transfer length across the surfaces of the target and it decreases with the increase of temperature. Supported by National Science Foundation of China (51371195, 11174358)

  2. Radiation Damage to the Nervous System: a delayed therapeutic hazard

    SciTech Connect

    Gilbert, H.A.; Kagan, A.R.

    1980-01-01

    This volume represents a good overview of an important issue - late effects of radiation on the nervous system, a topic of interest to everybody who deals with neurooncologic problems. The book is well edited and includes almost all relevant subjects ranging from diagnostic and dosimetric considerations to treatment of radiation brain necrosis.

  3. Cranial Radiation Therapy and Damage to Hippocampal Neurogenesis

    ERIC Educational Resources Information Center

    Monje, Michelle

    2008-01-01

    Cranial radiation therapy is associated with a progressive decline in cognitive function, prominently memory function. Impairment of hippocampal neurogenesis is thought to be an important mechanism underlying this cognitive decline. Recent work has elucidated the mechanisms of radiation-induced failure of neurogenesis. Potential therapeutic…

  4. Depopulation of metastable helium by radiative association with hydrogen and lithium ions

    SciTech Connect

    Augustovičová, L.; Soldán, P.; Kraemer, W. P.

    2014-02-10

    Depopulation of metastable He(2{sup 3}S) by radiative association with hydrogen and lithium ions is investigated using a fully quantal approach. Rate coefficients for spontaneous and stimulated radiative association of the HeH{sup +}, HeD{sup +}, and LiHe{sup +} molecular ions on the spin-triplet manifold are presented as functions of temperature considering the association to rotational-vibrational states of the lowest triplet electronic states a {sup 3}Σ{sup +} and b {sup 3}Σ{sup +} from the continuum states of the b {sup 3}Σ{sup +} electronic state. Evaluation of the rate coefficients is based on highly accurate quantum calculations, taking into account all possible state-to-state transitions at thermal energies (for spontaneous association) or at higher background energies (stimulated association). As expected, calculations show that the rate coefficients for radiative association to the a state are several orders of magnitude larger than the one for the b state formation. A noticeable effect by blackbody background radiation on the radiative association is only obtained for the b → b process. Aspects of the formation and abundance of the metastable HeH{sup +}(a {sup 3}Σ{sup +}) in astrophysical environments are briefly discussed.

  5. Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation

    PubMed Central

    Zhang, Xurui; Ye, Caiyong; Sun, Fang; Wei, Wenjun; Hu, Burong; Wang, Jufang

    2016-01-01

    Persistent DNA damage is considered as a main cause of cellular senescence induced by ionizing radiation. However, the molecular bases of the DNA damage and their contribution to cellular senescence are not completely clear. In this study, we found that both heavy ions and X-rays induced senescence in human uveal melanoma 92–1 cells. By measuring senescence associated-β-galactosidase and cell proliferation, we identified that heavy ions were more effective at inducing senescence than X-rays. We observed less efficient repair when DNA damage was induced by heavy ions compared with X-rays and most of the irreparable damage was complex of single strand breaks and double strand breaks, while DNA damage induced by X-rays was mostly repaired in 24 hours and the remained damage was preferentially associated with telomeric DNA. Our results suggest that DNA damage induced by heavy ion is often complex and difficult to repair, thus presents as persistent DNA damage and pushes the cell into senescence. In contrast, persistent DNA damage induced by X-rays is preferentially associated with telomeric DNA and the telomere-favored persistent DNA damage contributes to X-rays induced cellular senescence. These findings provide new insight into the understanding of high relative biological effectiveness of heavy ions relevant to cancer therapy and space radiation research. PMID:27187621

  6. Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation.

    PubMed

    Zhang, Xurui; Ye, Caiyong; Sun, Fang; Wei, Wenjun; Hu, Burong; Wang, Jufang

    2016-01-01

    Persistent DNA damage is considered as a main cause of cellular senescence induced by ionizing radiation. However, the molecular bases of the DNA damage and their contribution to cellular senescence are not completely clear. In this study, we found that both heavy ions and X-rays induced senescence in human uveal melanoma 92-1 cells. By measuring senescence associated-β-galactosidase and cell proliferation, we identified that heavy ions were more effective at inducing senescence than X-rays. We observed less efficient repair when DNA damage was induced by heavy ions compared with X-rays and most of the irreparable damage was complex of single strand breaks and double strand breaks, while DNA damage induced by X-rays was mostly repaired in 24 hours and the remained damage was preferentially associated with telomeric DNA. Our results suggest that DNA damage induced by heavy ion is often complex and difficult to repair, thus presents as persistent DNA damage and pushes the cell into senescence. In contrast, persistent DNA damage induced by X-rays is preferentially associated with telomeric DNA and the telomere-favored persistent DNA damage contributes to X-rays induced cellular senescence. These findings provide new insight into the understanding of high relative biological effectiveness of heavy ions relevant to cancer therapy and space radiation research. PMID:27187621

  7. Iatrogenic Damage to the Periodontium Caused by Radiation and Radiotherapy

    PubMed Central

    Kassim, Najeeb; Sirajuddin, Syed; Biswas, Shriparna; Rafiuddin, Syed; Apine, Ashwini

    2015-01-01

    The radio-sensitivity of a tissue or organ is measured by its response to irradiation. Loss of moderate numbers of cells does not affect the function of most organs. However, with loss of large numbers of cells, all affected organisms display a clinical result. The severity of this change depends on the dosage and thus the extent of cell loss. Moderate doses to a localized area may lead to repairable damage. Comparable doses to a whole organism may result in death from damage to the most sensitive systems in the body. PMID:26312083

  8. Radiation Damage Calculations for the FUBR and BEATRIX Irradiations of Lithium Compounds in EBR-II and FFTF

    SciTech Connect

    LR Greenwood

    1999-06-17

    The Fusion Breeder Reactor (FUBR) and Breeder Exchange Matrix (BEATRIX) experiments were cooperative efforts by members of the International Energy Agency to investigate the irradiation behavior of solid breeder materials for tritium production to support future fusion reactors. Lithium ceramic materials including Li{sub 2}O, LiAlO{sub 2}, Li{sub 4}SiO{sub 4}, and Li{sub 2}ZrO{sub 3} with varying {sup 6}Li enrichments from 0 to 95% were irradiated in a series of experiments in the Experimental Breeder Reactor (EBR II) and in the Fast Flux Test Facility (FFTF) over a period of about 10 years from 1982 to 1992. These experiments were characterized in terms of the nominal fast neutron fluences and measured {sup 6}Li burnup factors, as determined by either mass spectrometry or helium measurements. Radiation damage in these compounds is caused by both the {sup 6}Li-burnup reaction and by all other possible neutron reactions with the atoms in the compound materials. In this report, displacements per atom (dpa) values have been calculated for each type of material in each of the various irradiations that were conducted. Values up to 11% {sup 6}Li-burnup and 130 dpa are predicted for the longest irradiations. The dpa cross sections were calculated for each compound using the SPECOMP computer code. Details of the dpa calculations are presented in the report. Total dpa factors were determined with the SPECTER computer code by averaging the dpa cross sections over the measured or calculated neutron flux spectra for each series of irradiations. Using these new calculations, previously measured radiation damage effects in these lithium compounds can be compared or correlated with other irradiation data on the basis of the dpa factor as well as {sup 6}Li-burnup.

  9. Evidence of Dopant Type-Inversion and Other Radiation Damage Effects of the CDF Silicon Detectors

    SciTech Connect

    Martinez-Ballarin, Roberto

    2010-06-01

    The aim of this document is to study the effect of radiation damage on the silicon sensors. The reflection of the effect of radiation can be observed in two fundamental parameters of the detector: the bias current and the bias voltage. The leakage current directly affects the noise, while the bias voltage is required to collect the maximum signal deposited by the charged particle.

  10. DETECTION OF LOW DOSE RADIATION INDUCED DNA DAMAGE USING TEMPERATURE DIFFERENNTIAL FLUORESENCE ASSAY

    EPA Science Inventory

    A rapid and sensitive fluorescence assay for radiation-induced DNA damage is reported. Changes in temperature-induced strand separation in both calf thymus DNA and plasmid DNA (puc 19 plasmid from Escherichia coli) were measured after exposure to low doses of radiation. Exposures...

  11. DETECTION OF LOW DOSE RADIATION INDUCED DNA DAMAGE USING TEMPERATURE DIFFERENTIAL FLUORESCENCE ASSAY

    EPA Science Inventory

    A rapid and sensitive fluorescence assay for radiation-induced DNA damage is reported. Changes in temperature-induced strand separation in both calf thymus DNA and plasmid DNA (puc 19 plasmid from Escherichia coli) were measured after exposure to low doses of radiation. Exposur...

  12. Effect of radiation-induced damage on deuterium retention in tungsten, tungsten coatings and Eurofer

    NASA Astrophysics Data System (ADS)

    Ogorodnikova, O. V.; Sugiyama, K.

    2013-11-01

    An influence of radiation-induced damage on hydrogen isotope retention and transport in a bulk tungsten (W), dense nano-structured W coatings and Eurofer was investigated under well-defined laboratory conditions. Radiation-induced defects in W materials and Eurofer were created by irradiation with 20 MeV W ions. Following the damage production, samples were exposed to low-energy deuterium plasma. The deuterium (D) retention in each sample was subsequently measured by nuclear reaction analysis (NRA) for the depth profiling up to 6 μm. It was shown that the D retention at radiation-induced damage is almost equivalent for different W grades after irradiation at high enough fluence. The kinetic of D migration and trapping in damaged area as well as recovery of radiation-induced damage were investigated by loading at different temperatures. It was shown that deuterium retention in tungsten in fusion environment will be dominated by radiation-induced effect in a wide range of investigated temperatures, namely, from room temperature to 1100 K. Whereas displacement damage produced in Eurofer has less pronounced effect on the deuterium accumulation.

  13. Periodic annealing of radiation damage in GaAs solar cells

    NASA Technical Reports Server (NTRS)

    Loo, R. Y.; Knechtli, R. C.; Kamath, G. S.

    1980-01-01

    Continuous annealing of GaAs solar cells is compared with periodic annealing to determine their relative effectiveness in minimizing proton radiation damage. It is concluded that continuous annealing of the cells in space at 150 C can effectively reduce the proton radiation damage to the GaAs solar cells. Periodic annealing is most effective if it can be initiated at relatively low fluences (approximating continuous annealing), especially if low temperatures of less than 200 C are to be used. If annealing is started only after the fluence of the damaging protons has accumulated to a high value 10 to the 11th power sq/pcm), effective annealing is still possible at relatively high temperatures. Finally, since electron radiation damage anneals even more easily than proton radiation damage, substantial improvements in GaAs solar cell life can be achieved by incorporating the proper annealing capabilities in solar panels for practical space missions where both electron and proton radiation damage have to be minimized.

  14. Prevent Eye Damage: Protect Yourself from UV Radiation

    MedlinePlus

    ... exposure to UV radiation from daily activities, including reflections off of snow, pavement, and other surfaces, can ... by a day at the beach without sunglasses; reflections off of snow, water, or concrete; or exposure ...

  15. Proton irradiation of stem cells: Radiation damage and chemical radioprotection

    NASA Technical Reports Server (NTRS)

    Riley, R. C.; Montour, J. L.; Gurney, C. W.

    1972-01-01

    Effects of high energy protons on erythropoietic stem cells and radioprotection by chemicals were investigated in NASA Space Radiation Effects Laboratory. The effects of a parallel beam of 600 MeV protons. The fluence, when converted to dose, were referenced to the synchrocyclotron beam monitors which were then used to administer radiation exposures. Mice were given graded doses to 300 rads to determine dose-response curve. Other mice received saline, AET, or 5-hydroxytryptamine 10 to 15 minutes before exposure.

  16. Dark progression reveals slow timescales for radiation damage between T = 180 and 240 K

    PubMed Central

    Warkentin, Matthew; Badeau, Ryan; Hopkins, Jesse; Thorne, Robert E.

    2011-01-01

    Can radiation damage to protein crystals be ‘outrun’ by collecting a structural data set before damage is manifested? Recent experiments using ultra-intense pulses from a free-electron laser show that the answer is yes. Here, evidence is presented that significant reductions in global damage at temperatures above 200 K may be possible using conventional X-ray sources and current or soon-to-be available detectors. Specifically, ‘dark progression’ (an increase in damage with time after the X-rays have been turned off) was observed at temperatures between 180 and 240 K and on timescales from 200 to 1200 s. This allowed estimation of the temperature-dependent timescale for damage. The rate of dark progression is consistent with an Arrhenius law with an activation energy of 14 kJ mol−1. This is comparable to the activation energy for the solvent-coupled diffusive damage processes responsible for the rapid increase in radiation sensitivity as crystals are warmed above the glass transition near 200 K. Analysis suggests that at T = 300 K data-collection times of the order of 1 s (and longer at lower temperatures) may allow significant reductions in global radiation damage, facilitating structure solution on crystals with liquid solvent. No dark progression was observed below T = 180 K, indicating that no important damage process is slowed through this timescale window in this temperature range. PMID:21904032

  17. Ionizing radiation, antioxidant response and oxidative damage: A meta-analysis.

    PubMed

    Einor, D; Bonisoli-Alquati, A; Costantini, D; Mousseau, T A; Møller, A P

    2016-04-01

    One mechanism proposed as a link between exposure to ionizing radiation and detrimental effects on organisms is oxidative damage. To test this hypothesis, we surveyed the scientific literature on the effects of chronic low-dose ionizing radiation (LDIR) on antioxidant responses and oxidative damage. We found 40 publications and 212 effect sizes for antioxidant responses and 288 effect sizes for effects of oxidative damage. We performed a meta-analysis of signed and unsigned effect sizes. We found large unsigned effects for both categories (0.918 for oxidative damage; 0.973 for antioxidant response). Mean signed effect size weighted by sample size was 0.276 for oxidative damage and -0.350 for antioxidant defenses, with significant heterogeneity among effects for both categories, implying that ionizing radiation caused small to intermediate increases in oxidative damage and small to intermediate decreases in antioxidant defenses. Our estimates are robust, as shown by very high fail-safe numbers. Species, biological matrix (tissue, blood, sperm) and age predicted the magnitude of effects for oxidative damage as well as antioxidant response. Meta-regression models showed that effect sizes for oxidative damage varied among species and age classes, while effect sizes for antioxidant responses varied among species and biological matrices. Our results are consistent with the description of mechanisms underlying pathological effects of chronic exposure to LDIR. Our results also highlight the importance of resistance to oxidative stress as one possible mechanism associated with variation in species responses to LDIR-contaminated areas. PMID:26851726

  18. Phytochemicals for prevention of solar ultraviolet radiation-induced damages.

    PubMed

    Adhami, Vaqar M; Syed, Deeba N; Khan, Naghma; Afaq, Farrukh

    2008-01-01

    While solar light is indispensable for sustenance of life, excessive exposure can cause several skin-related disorders. The UV part of solar radiation, in particular, is linked to disorders ranging from mild inflammatory effects of the skin to as serious as causing several different types of cancers. Changes in lifestyle together with depletion in the atmospheric ozone layer during the last few decades have led to an increase in the incidence of skin cancer. Skin cancers consisting of basal and squamous cell carcinomas are especially linked to the UVB part of solar radiation. Reducing excessive exposure to solar radiation is desirable; however, as this approach is unavoidable, it is suggested that other novel strategies be developed to reduce the effects of solar radiation to skin. One approach to reduce the harmful effects of solar radiation is through the use of phytochemicals, an approach that is popularly known as "Photochemoprotection." In recent years many phytochemicals with potential antioxidant properties have been identified and found to be photoprotective in nature. We describe here some of the most popular phytochemicals being studied that have the potential to reduce the harmful effects associated with solar UV radiation. PMID:18266816

  19. Comparative study of radiation damage accumulation in Cu and Fe

    NASA Astrophysics Data System (ADS)

    Caturla, M. J.; Soneda, N.; Alonso, E.; Wirth, B. D.; Díaz de la Rubia, T.; Perlado, J. M.

    2000-01-01

    Bcc and fcc metals exhibit significant differences in behavior when exposed to neutron or heavy ion irradiation. Transmission electron microscopy (TEM) observations reveal that damage in the form of stacking fault tetrahedra (SFT) is visible in copper irradiated to very low doses, but that no damage is visible in iron irradiated to the same total dose. In order to understand and quantify this difference in behavior, we have simulated damage production and accumulation in fcc Cu and bcc Fe. We use 20 keV primary knock-on atoms (PKAs) at a homologous temperature of 0.25 of the melting point. The primary damage state was calculated using molecular dynamics (MD) with empirical, embedded-atom interatomic potentials. Damage accumulation was modeled using a kinetic Monte Carlo (kMC) algorithm to follow the evolution of all defects produced in the cascades. The diffusivities and binding energies of defects are input data for this simulation and were either extracted from experiments, the literature, or calculated using MD. MD simulations reveal that vacancy clusters are produced within the cascade core in the case of copper. In iron, most of the vacancies do not cluster during cooling of the cascade core and are available for diffusion. In addition, self-interstitial atom (SIA) clusters are produced in copper cascades but those observed in iron are smaller in number and size. The combined MD/kMC simulations reveal that the visible cluster densities obtained as a function of dose are at least one order of magnitude lower in Fe than in Cu. We compare the results with experimental measurements of cluster density and find excellent agreement between the simulations and experiments when small interstitial clusters are considered to be mobile as suggested by recent MD simulations.

  20. The radiation damage of crystalline silicon PN diode in tritium beta-voltaic battery.

    PubMed

    Lei, Yisong; Yang, Yuqing; Liu, Yebing; Li, Hao; Wang, Guanquan; Hu, Rui; Xiong, Xiaoling; Luo, Shunzhong

    2014-08-01

    A tritium beta-voltaic battery using a crystalline silicon convertor composed of (100)Si/SiO2/Si3N4 film degrades remarkably with radiation from a high intensity titanium tritide film. Simulation and experiments were carried out to investigate the main factor causing the degradation. The radiation damages mainly comes from the x-ray emitted from the titanium tritide film and beta particle can relieve the damages. The x-ray radiation induced positive charges in the SiO2 film destroying the output property of the PN diode with the induction of an electric field. PMID:24751350

  1. A thermochemical model of radiation damage and annealing applied to GaAs solar cells

    NASA Technical Reports Server (NTRS)

    Conway, E. J.; Walker, G. H.; Heinbockel, J. H.

    1981-01-01

    Calculations of the equilibrium conditions for continuous radiation damage and thermal annealing are reported. The calculations are based on a thermochemical model developed to analyze the incorporation of point imperfections in GaAs, and modified by introducing the radiation to produce native lattice defects rather than high-temperature and arsenic atmospheric pressure. The concentration of a set of defects, including vacancies, divacancies, and impurity vacancy complexes, are calculated as a function of temperature. Minority carrier lifetimes, short circuit current, and efficiency are deduced for a range of equilibrium temperatures. The results indicate that GaAs solar cells could have a mission life which is not greatly limited by radiation damage.

  2. Proton radiation damage in high-resistivity n-type silicon CCDs

    SciTech Connect

    Bebek, C.J.; Groom, D.E.; Holland, S.E.; Karcher, A.; Kolbe, W.F.; Lee, J.; Levi, M.E.; Palaio, N.P.; Turko, B.T.; Uslenghi, M.C.; Wagner, M.T.; Wang, G.

    2001-12-20

    A new type of p-channel CCD constructed on high-resistivity n-type silicon was exposed to 12 MeV protons at doses up to 1x1011 protons/cm2. The charge transfer efficiency was measured as a function of radiation dose and temperature. We previously reported that these CCDs are significantly more tolerant to radiation damage than conventional n-channel devices. In the work reported here, we used pocket pumping techniques and charge transfer efficiency measurements to determine the identity and concentrations of radiation induced traps present in the damaged devices.

  3. DNA Damage by Ionizing Radiation: Tandem Double Lesions by Charged Particles

    NASA Technical Reports Server (NTRS)

    Huo, Winifred M.; Chaban, Galina M.; Wang, Dunyou; Dateo, Christopher E.

    2005-01-01

    Oxidative damages by ionizing radiation are the source of radiation-induced carcinogenesis, damage to the central nervous system, lowering of the immune response, as well as other radiation-induced damages to human health. Monte Carlo track simulations and kinetic modeling of radiation damages to the DNA employ available molecular and cellular data to simulate the biological effect of high and low LET radiation io the DNA. While the simulations predict single and double strand breaks and base damages, so far all complex lesions are the result of stochastic coincidence from independent processes. Tandem double lesions have not yet been taken into account. Unlike the standard double lesions that are produced by two separate attacks by charged particles or radicals, tandem double lesions are produced by one single attack. The standard double lesions dominate at the high dosage regime. On the other hand, tandem double lesions do not depend on stochastic coincidences and become important at the low dosage regime of particular interest to NASA. Tandem double lesions by hydroxyl radical attack of guanine in isolated DNA have been reported at a dosage of radiation as low as 10 Gy. The formation of two tandem base lesions was found to be linear with the applied doses, a characteristic of tandem lesions. However, tandem double lesions from attack by a charged particle have not been reported.

  4. Hot HB Stars in Globular Clusters: Physical Parameters and Consequences for Theory. 5; Radiative Levitation Versus Helium Mixing

    NASA Technical Reports Server (NTRS)

    Moehler, S.; Sweigart, A. V.; Landsman, W. B.; Heber, U.

    2000-01-01

    Atmospheric parameters (T(sub eff), log g), masses and helium abundances are derived for 42 hot horizontal branch (HB) stars in the globular cluster NGC6752. For 19 stars we derive magnesium and iron abundances as well and find that iron is enriched by a factor of 50 on average with respect to the cluster abundance whereas the magnesium abundances are consistent with the cluster abundance. Radiation pressure may levitate heavy elements like iron to the surface of the star in a diffusive process. Taking into account the enrichment of heavy elements in our spectroscopic analyses we find that high iron abundances can explain part, but not all, of the problem of anomalously low gravities along the blue HB. The blue HB stars cooler than about 15,100 K and the sdB stars (T(sub eff) greater than or = 20,000 K) agree well with canonical theory when analysed with metal-rich ([M/H] = +0.5) model atmospheres, but the stars in between these two groups remain offset towards lower gravities and masses. Deep Mixing in the red giant progenitor phase is discussed as another mechanism that may influence the position of the blue HB stars in the (T(sub eff), log g)-plane but not their masses.

  5. Atomistic studies of formation and diffusion of helium clusters and bubbles in BCC iron

    SciTech Connect

    Stewart, David M; Stoller, Roger E; Osetskiy, Yury N

    2011-01-01

    In fusion applications, helium created by transmutation plays an important role in the response of reduced-activation ferritic/martensitic (RAFM) steels to neutron radiation damage. We have performed extensive atomistic simulations using the ORNL 3-body Fe He interatomic potential combined with three interatomic potentials for the iron matrix. Some of the results obtained are summarized in this review. Interstitial helium is very mobile and coalesces together to form interstitial clusters. We have investigated the mobility of these clusters. When an interstitial He cluster reaches sufficient size, it punches out an Fe interstitial, creating an immobile helium vacancy cluster. If more helium atoms join it, more Fe interstitials can be created; the He V defect is a nascent bubble. These mechanisms are investigated together in simulations that examine the nucleation of He defects. Mobile interstitial He clusters and helium bubbles 1 to 6 nm across are also simulated separately. Results are compared based on temperature and interatomic potentials used.

  6. Helium-neon effects of laser radiation in rats infected with thromboxane B2

    NASA Astrophysics Data System (ADS)

    Juri, Hugo; Palma, J. A.; Campana, Vilma; Gavotto, A.; Lapin, R.; Yung, S.; Lillo, J.

    1991-06-01

    In previous investigations it was found that prostaglanding E1 (PGE1) and Bradiquinine (B), liberated in the inflammatory process, produced a significant increment on the Plasma fibrition Level (PFL), indicative of inflammatory process. The mentioned increment was completely abolished by the irradiation with HeNe laser in the area of infection of the mentioned substances. In the current investigation it was studied the effect of HeNe laser radiation on the P.F.L. of rats injected with another substance related with the inflammatory process and tissular injury: Thromboxane (Tx). It is though that the signal to increase P.F.L. is though an adrenal and an extra adrenal pathways. To study it we injected normal and medullectomized animals and both showed marked increment of P.F.L. Then we repeated the experiment but followed immediately by HeNe laser radiation and we noted a complete blockage of the P.F.L. increment in both groups which suggest that the effect is extra-adrenal. All substances were injected I.M. once daily X 3 days. Immediately after injection the area was irradiated with HeNe laser, 1.5 J total energy. In the normal, non injected non irradiated animals the P.F.L. reached 210.3 + 1.15 mg%. The single injection of Tx did not modify the P.F.L. compared with the previous group. The HeNe irradiation alone did not modify the P.F.L. in the animals injected with Tx only. The animals injected with PGE1+B showed a marked increment of P.F.L. to 337.6 + 14.5 mg%; the HeNe laser radiation completely abolished the increment (231 + 22.3 mg%). But in the animals injected with PGE1+B+Tx, the P.F.L. reached even larger values: 375.2 + 15.3 mg%. The HeNe laser radiation produced a partial blockage in P.F.L. increment (270.3 +/- 13.4 mg%). Showing a significant difference (p < 0.001) compared with normal rats or with rats injected with PGE1+B+Tx without radiation. In conclusion Tx potentiate the effect of PGE+B on the P.F.L. The HeNe laser blocks completely the interaction

  7. Experimental studies of radiation damage of silicon detectors. Internal report

    SciTech Connect

    Angelescu, T.; Ghete, V.M.; Ghiordanescu, N.; Lazanu, I.; Mihul, A.; Golutvin, I.; Lazanu, S.; Savin, I.; Vasilescu, A.; Biggeri, U.; Borchi, E.; Bruzzi, M. |; Li, Z.; Kraner, H.W.

    1994-02-01

    New particle physics experiments are correlated with high luminosity and/or high energy. The new generation of colliding beam machines which will be constructed will make an extrapolation of a factor of 100 in the center of mass energy and of 1000 in luminosity beyond present accelerators. The scientific community hopes that very exciting physics results could be achieved this way, from the solution to the problem of electroweak symmetry breaking to the possible discovery of new, unpredicted phenomena. The particles which compose the radiation field are: electrons, pions, neutrons, protons and photons. It has become evident that the problem of the radiation resistance of detectors in this severe environment is a crucial one. This situation is complicated more by the fact that detectors must work all the run time of the machine, and better all the time of the experiment, without replacement (part or whole). So, studies related to the investigation of the radiation hardness of all detector parts, are developing. The studies are in part material and device characterization after irradiation, and in part technological developments, made in order to find harder, cheaper technologies, for larger surfaces. Semiconductor detectors have proven to be a good choice for vertex and calorimeter. Both fixed target machines and colliders had utilized in the past silicon junction detectors as the whole or part of the detection system. Precision beam hodoscopes and sophisticated trigger devices with silicon are equally used. The associated electronics in located near the detectors, and is subjected to the same radiation fields. Studies of material and device radiation hardness are developing in parallel. Here the authors present results on the radiation hardness of silicon, both as a bulk material and as detectors, to neutron irradiation at high fluences.

  8. Radiation damage in proton irradiated indium phosphide solar cells

    NASA Technical Reports Server (NTRS)

    Weinberg, I.; Swartz, C. K.; Hart, R. E., Jr.; Yamaguchi, Masafumi

    1986-01-01

    Indium phosphide solar cells exposed to 10 MeV proton irradiations were found to have significantly greater radiation resistance than either GaAs or Si. Performance predictions were obtained for two proton dominated orbits and one in which both protons and electrons were significant cell degradation factors. Array specific power was calculated using lightweight blanket technology, a SEP array structure, and projected cell efficiencies. Results indicate that arrays using fully developed InP cells should out-perform those using GaAs or Si in orbits where radiation is a significant cell degradation factor.

  9. Asymmetry of radiation damage properties in Al-Ti nanolayers

    SciTech Connect

    Setyawan, Wahyu; Gerboth, Matthew D.; Yao, Bo; Henager, Charles H.; Devaraj, Arun; Vemuri, Venkata Rama Ses; Thevuthasan, Suntharampillai; Shutthanandan, V.

    2014-02-01

    Molecular dynamics (MD) simulations were employed with empirical potentials to study the effects of multilayer interfaces and interface spacing in Al-Ti nanolayers. Several model interfaces derived from stacking of close-packed layers or face-centered cubic \\{100\\} layers were investigated. The simulations reveal significant and important asymmetries in defect production with $\\sim$60\\% of vacancies created in Al layers compared to Ti layers within the Al-Ti multilayer system. The asymmetry in the creation of interstitials is even more pronounced. The asymmetries cause an imbalance in the ratio of vacancies and interstitials in films of dissimilar materials leading to $>$90\\% of the surviving interstitials located in the Al layers. While in the close-packed nanolayers the interstitials migrate to the atomic layers adjacent to the interface of the Al layers, in the \\{100\\} nanolayers the interstitials migrate to the center of the Al layers and away from the interfaces. The degree of asymmetry and defect ratio imbalance increases as the layer spacing decreases in the multilayer films. Underlying physical processes are discussed including the interfacial strain fields and the individual elemental layer stopping power in nanolayered systems. In addition, experimental work was performed on low-dose (10$^{16}$ atoms/cm$^2$) helium (He) irradiation on Al/Ti nanolayers (5 nm per film), resulting in He bubble formation $\\sim$1 nm in diameter in the Ti film near the interface. The correlation between the preferential flux of displaced atoms from Ti films to Al films during the defect production that is revealed in the simulations and the morphology and location of He bubbles from the experiments is discussed.

  10. Simultaneous quiet time observations of energetic radiation belt protons and helium ions - The equatorial alpha/p ratio near 1 MeV

    NASA Technical Reports Server (NTRS)

    Fritz, T. A.; Spjeldvik, W. N.

    1979-01-01

    Simultaneous monitoring of energetic helium ions and protons in the earth's radiation belts has been conducted with Explorer 45 in the immediate vicinity of the equatorial plane. Protons were measured from less than 1 keV to 1.6 MeV and also above 3.3 MeV in a channel responsive up to 22 MeV; helium ions were monitored in three passbands: 910 keV to 3.15 MeV, 590 to 910 keV, and 2.0 to 3.99 MeV. Alpha/proton flux ratios were found to vary significantly with energy and location in the radiation belts. At equal energy per nucleon a range of variability for alpha/p from 0.0001 to well above 0.001 was found, and at equal energy per ion the corresponding variability was from 0.001 to above 10. The latter findings emphasize the relative importance of the very energetic helium ions in the overall radiation belt ion populations.

  11. Clustered DNA damages induced by high and low LET radiation, including heavy ions

    NASA Technical Reports Server (NTRS)

    Sutherland, B. M.; Bennett, P. V.; Schenk, H.; Sidorkina, O.; Laval, J.; Trunk, J.; Monteleone, D.; Sutherland, J.; Lowenstein, D. I. (Principal Investigator)

    2001-01-01

    Clustered DNA damages--here defined as two or more lesions (strand breaks, oxidized purines, oxidized pyrimidines or abasic sites) within a few helical turns--have been postulated as difficult to repair accurately, and thus highly significant biological lesions. Further, attempted repair of clusters may produce double strand breaks (DSBs). However, until recently, there was no way to measure ionizing radiation-induced clustered damages, except DSB. We recently described an approach for measuring classes of clustered damages (oxidized purine clusters, oxidized pyrimidine clusters, abasic clusters, along with DSB). We showed that ionizing radiation (gamma rays and Fe ions, 1 GeV/amu) does induce such clusters in genomic DNA in solution and in human cells. These studies also showed that each damage cluster results from one radiation hit (and its track), thus indicating that they can be induced by very low doses of radiation, i.e. two independent hits are not required for cluster induction. Further, among all complex damages, double strand breaks comprise--at most-- 20%, with the other clustered damages being at least 80%.

  12. Radiation damage in MOS integrated circuits, Part 1

    NASA Technical Reports Server (NTRS)

    Danchenko, V.

    1971-01-01

    Complementary and p-channel MOS integrated circuits made by four commercial manufacturers were investigated for sensitivity to radiation environment. The circuits were irradiated with 1.5 MeV electrons. The results are given for electrons and for the Co-60 gamma radiation equivalent. The data are presented in terms of shifts in the threshold potentials and changes in transconductances and leakages. Gate biases of -10V, +10V and zero volts were applied to individual MOS units during irradiation. It was found that, in most of circuits of complementary MOS technologies, noticable changes due to radiation appear first as increased leakage in n-channel MOSFETs somewhat before a total integrated dose 10 to the 12th power electrons/sg cm is reached. The inability of p-channel MOSFETs to turn on sets in at about 10 to the 13th power electrons/sq cm. Of the circuits tested, an RCA A-series circuit was the most radiation resistant sample.

  13. Thermal and radiation damage to SL/1 EREP films

    NASA Technical Reports Server (NTRS)

    Perry, L.

    1973-01-01

    Tests were conducted to determine the present sensitometric characteristics of the SL/1 EREP films stored in Skylab. These films underwent the high temperature environment at the beginning of the mission and have since been stored outside the film vault. As a result, the films will have received a radiation dose estimated at approximately 12 rads by the end of SL/3.

  14. Feasibility of OCT to detect radiation-induced esophageal damage in small animal models (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Jelvehgaran, Pouya; Alderliesten, Tanja; Salguero, Javier; Borst, Gerben; Song, Ji-Ying; van Leeuwen, Ton G.; de Boer, Johannes F.; de Bruin, Daniel M.; van Herk, Marcel B.

    2016-03-01

    Lung cancer survival is poor and radiotherapy patients often suffer serious treatment side effects. The esophagus is particularly sensitive leading to reduced food intake or even fistula formation. Only few direct techniques exist to measure radiation-induced esophageal damage, for which knowledge is needed to improve the balance between risk of tumor recurrence and complications. Optical coherence tomography (OCT) is a minimally-invasive imaging technique that obtains cross-sectional, high-resolution (1-10µm) images and is capable of scanning the esophageal wall up to 2-3mm depth. In this study we investigated the feasibility of OCT to detect esophageal radiation damage in mice. In total 30 mice were included in 4 study groups (1 main and 3 control groups). Mice underwent cone-beam CT imaging for initial setup assessment and dose planning followed by single-fraction dose delivery of 4, 10, 16, and 20Gy on 5mm spots, spaced 10mm apart. Mice were repeatedly imaged using OCT: pre-irradiation and up to 3 months post-irradiation. The control groups received either OCT only, irradiation only, or were sham-operated. We used histopathology as gold standard for radiation-induced damage diagnosis. The study showed edema in both the main and OCT-only groups. Furthermore, radiation-induced damage was primarily found in the highest dose region (distal esophagus). Based on the histopathology reports we were able to identify the radiation-induced damage in the OCT images as a change in tissue scattering related to the type of induced damage. This finding indicates the feasibility and thereby the potentially promising role of OCT in radiation-induced esophageal damage assessment.

  15. Analyses of the Secondary Particle Radiation and the DNA Damage it Causes to Human Keratinocytes

    SciTech Connect

    Lebel E. A.; Tafrov S.; Rusek, A.; Sivertz, M. B.; Yip, K.; Thompson, K. H.

    2011-11-01

    High-energy protons, and high mass and energy ions, along with the secondary particles they produce, are the main contributors to the radiation hazard during space explorations. Skin, particularly the epidermis, consisting mainly of keratinocytes with potential for proliferation and malignant transformation, absorbs the majority of the radiation dose. Therefore, we used normal human keratinocytes to investigate and quantify the DNA damage caused by secondary radiation. Its manifestation depends on the presence of retinol in the serum-free media, and is regulated by phosphatidylinositol 3-kinases. We simulated the generation of secondary radiation after the impact of protons and iron ions on an aluminum shield. We also measured the intensity and the type of the resulting secondary particles at two sample locations; our findings agreed well with our predictions. We showed that secondary particles inflict DNA damage to different extents, depending on the type of primary radiation. Low-energy protons produce fewer secondary particles and cause less DNA damage than do high-energy protons. However, both generate fewer secondary particles and inflict less DNA damage than do high mass and energy ions. The majority of cells repaired the initial damage, as denoted by the presence of 53BPI foci, within the first 24 hours after exposure, but some cells maintained the 53BP1 foci longer.

  16. Analyses of the secondary particle radiation and the DNA damage it causes to human keratinocytes

    SciTech Connect

    Lebel E.; Rusek A.; Sivertz, M.; Yip, K.; Thompson, K.; Tafrov, S.

    2011-11-22

    High-energy protons, and high mass and energy ions, along with the secondary particles they produce, are the main contributors to the radiation hazard during space explorations. Skin, particularly the epidermis, consisting mainly of keratinocytes with potential for proliferation and malignant transformation, absorbs the majority of the radiation dose. Therefore, we used normal human keratinocytes to investigate and quantify the DNA damage caused by secondary radiation. Its manifestation depends on the presence of retinol in the serum-free media, and is regulated by phosphatidylinositol 3-kinases. We simulated the generation of secondary radiation after the impact of protons and iron ions on an aluminum shield. We also measured the intensity and the type of the resulting secondary particles at two sample locations; our findings agreed well with our predictions. We showed that secondary particles inflict DNA damage to different extents, depending on the type of primary radiation. Low-energy protons produce fewer secondary particles and cause less DNA damage than do high-energy protons. However, both generate fewer secondary particles and inflict less DNA damage than do high mass and energy ions. The majority of cells repaired the initial damage, as denoted by the presence of 53BPI foci, within the first 24 hours after exposure, but some cells maintained the 53BP1 foci longer.

  17. Mitochondria regulate DNA damage and genomic instability induced by high LET radiation

    NASA Astrophysics Data System (ADS)

    Zhang, Bo; Davidson, Mercy M.; Hei, Tom K.

    2014-04-01

    High linear energy transfer (LET) radiation including α particles and heavy ions is the major type of radiation found in space and is considered a potential health risk for astronauts. Even though the chance that these high LET particles traversing through the cytoplasm of cells is higher than that through the nuclei, the contribution of targeted cytoplasmic irradiation to the induction of genomic instability and other chromosomal damages induced by high LET radiation is not known. In the present study, we investigated whether mitochondria are the potential cytoplasmic target of high LET radiation in mediating cellular damage using a mitochondrial DNA (mtDNA) depleted (ρ0) human small airway epithelial (SAE) cell model and a precision charged particle microbeam with a beam width of merely one micron. Targeted cytoplasmic irradiation by high LET α particles induced DNA oxidative damage and double strand breaks in wild type ρ+ SAE cells. Furthermore, there was a significant increase in autophagy and micronuclei, which is an indication of genomic instability, together with the activation of nuclear factor kappa-B (NF-κB) and mitochondrial inducible nitric oxide synthase (iNOS) signaling pathways in ρ+ SAE cells. In contrast, ρ0 SAE cells exhibited a significantly lower response to these same endpoints examined after cytoplasmic irradiation with high LET α particles. The results indicate that mitochondria are essential in mediating cytoplasmic radiation induced genotoxic damage in mammalian cells. Furthermore, the findings may shed some light in the design of countermeasures for space radiation.

  18. Mitochondria regulate DNA damage and genomic instability induced by high LET radiation

    PubMed Central

    Zhang, Bo; Davidson, Mercy M.; Hei, Tom K.

    2014-01-01

    High linear energy transfer (LET) radiation including α particles and heavy ions is the major type of radiation find in space and is considered a potential health risk for astronauts. Even though the chance that these high LET particles traversing through the cytoplasm of cells is higher than that through the nuclei, the contribution of targeted cytoplasmic irradiation, to the induction of genomic instability and other chromosomal damages induced by high LET radiation is not known. In the present study, we investigated whether mitochondria are the potential cytoplasmic target of high LET radiation in mediating cellular damage using a mitochondrial DNA (mtDNA) depleted (ρ0) human small airway epithelial (SAE) cell model and a precision charged particle microbeam with a beam width of merely one micron. Targeted cytoplasmic irradiation by high LET α particles induced DNA oxidative damage and double strand breaks in wild type ρ+ SAE cells. Furthermore, there was a significant increase in autophagy, micronuclei, which is an indication of genomic instability, together with the activation of nuclear factor kappa-B (NF-κB) and mitochondrial inducible nitric oxide synthase (iNOS) signaling pathways in ρ+ SAE cells. In contrast, ρ0 SAE cells exhibited a significantly lower response to these same endpoints examined after cytoplasmic irradiation with high LET α particles. The results indicate that mitochondria are essential in mediating cytoplasmic radiation induced genotoxic damage in mammalian cells. Furthermore, the findings may shed some light in the design of countermeasures for space radiation. PMID:25072018

  19. Radiation damage in diagnostic window materials for the TFTR

    SciTech Connect

    Primak, W.

    1981-07-01

    The general problem of evaluating diagnostic window materials for the TFTR at the tank wall location is described. Specific evaluations are presented for several materials: vitreous silica, crystal quartz, sapphire, zinc selenide, and several fluorides: lithium fluoride, magnesium fluoride, and calcium fluoride; and seal glasses are discussed. The effects of the neutrons will be minimal. The major problems arise from the high flux of ionizing radiation, mainly the soft x rays which are absorbed near the surface of the materials. Additionally, this large energy deposition causes a significant thermal pulse with attendant thermal stresses. It is thus desirable to protect the windows with cover slips where this is feasible or to reduce the incident radiation by mounting the windows on long pipes. A more detailed summary is given at the end of this report.

  20. An Assessment of Radiation Damage Models and Methods

    SciTech Connect

    Stoller, Roger E; Mansur, Louis K

    2005-05-01

    The current state of development of the primary models used for investigating and simulating irradiation effects in structural alloys of interest to the U.S. DOE's Generation-IV reactor program are discussed. The underlying theory that supports model development is also described where appropriate. First, the key processes that underlie radiation-induced changes in material properties are summarized, and the types of radiation effects that subsequently arise are described. Future development work needed in order for theory, modeling, and computational materials science to support and add value to the Gen IV reactor materials program are then outlined. The expected specific outcomes and overall benefits of the required effort are: the knowledge to extrapolate material behavior to conditions for which there are no experimental data; systematic understanding of mechanisms and processes to enable confident interpolation between point-by-point experimental observations; acceleration of the development, selection, and qualification of materials for reactor service; and prediction of material response to real-world operating load histories which often involve a complicated superposition of time, temperature, radiation dose rate, and mechanical loading conditions. Opportunities for international collaboration to accelerate progress in all of the required research areas are briefly discussed, particularly in the context of two well coordinated, broad-based research projects on modeling and simulation of radiation effects on materials that are currently funded in Europe. In addition to providing the opportunity for substantial leveraging of the DOE-funded activities in this area, these projects may serve as models for future development within the Gen-IV program. The larger of these two projects, which involves 12 European research laboratories and 16 universities, is called PERFECT and is funded by the European Union. A smaller effort focusing on developing predictive

  1. Radiative HELIUM-3 Capture by CARBON-12 to Excited States in OXYGEN-15

    NASA Astrophysics Data System (ADS)

    Abduljalil, Amir M.

    The radiative ^3He capture reaction on ^{12}C was measured over the energy region E_{ rm 3_{He}} = 12-25 MeV. The gamma-ray was measured by two 25.4 cm long and 25.4 cm in diameter NaI(Tl) spectrometers surrounded by anticoincidence plastic shields. Excitation functions at theta_{rm lab} = 60^circ and 105 ^circ have been measured for captures populating the ground and various excited states of ^{15}O. Angular distribution have been measured at E_{rm 3 _{He}} = 18.9 MeV. The existence of giant resonances built on the excited states of ^{15}O was investigated. Calculations from a phenomenological direct-semidirect cluster model were performed to bound and unbound final states in ^{15}O to determine the magnitude and the energy dependence of the cross section as well as the angular distributions. The calculations showed a reasonable agreement with the excitation function.

  2. Potential for radiation damage to carbon steel storage tanks for high level radioactive waste

    SciTech Connect

    Caskey, G.R. Jr.; Sindelar, R.L.; Thomas, J.K.

    1993-07-30

    A low intensity radiation field is generated by the high level waste that is stored within carbon steel lined tanks at the Savannah River Site (SRS). The highest level of radiation damage to the tank walls from gamma and spontaneous neutron emissions is estimated to be less than 1.0E-6 displacements per atom (DPA) for a 100 year exposure to fresh, ``high heat`` SRS waste assuming continuous replenishment of the radionuclides. This damage level is below the limit for measurable radiation damage to the mechanical properties of carbon steel. Structural assessment of tanks for storage of high level waste may be based on nominal or code values of the mechanical properties of the steels from which the tanks were constructed.

  3. Review of radiation damage in GaN-based materials and devices

    SciTech Connect

    Pearton, Stephen J.; Deist, Richard; Ren, Fan; Liu, Lu; Polyakov, Alexander Y.; Kim, Jihyun

    2013-09-15

    A review of the effects of proton, neutron, γ-ray, and electron irradiation on GaN materials and devices is presented. Neutron irradiation tends to create disordered regions in the GaN, while the damage from the other forms of radiation is more typically point defects. In all cases, the damaged region contains carrier traps that reduce the mobility and conductivity of the GaN and at high enough doses, a significant degradation of device performance. GaN is several orders of magnitude more resistant to radiation damage than GaAs of similar doping concentrations. In terms of heterostructures, preliminary data suggests that the radiation hardness decreases in the order AlN/GaN > AlGaN/GaN > InAlN/GaN, consistent with the average bond strengths in the Al-based materials.

  4. Modification of high LET radiation-induced damage and its repair in yeast by hypoxia.

    PubMed

    Subrahmanyam, P; Rao, B S; Reddy, N M; Murthy, M S; Madhvanath, U

    1979-11-01

    The lethal response of a diploid yeast strain BZ34 to densely ionizing radiations from the reaction 10B(n, alpha)7 Li was studied. The values for relative biological effectiveness (r.b.e.) and oxygen enhancement ratio (o.e.r.) for this radiation compare favourably with the data obtained with charged particles on the same strain of yeast. Recovery from potentially lethal damage was also studied by post-irradiation holding under non-nutrient conditions. In order to understand the role of oxygen in the recovery process, the investigation covered the following treatment regimens: (a) aerobic irradiation and aerobic holding (A-A), (b) aerobic irradiation and hypoxic holding (A-H), (c) hypoxic irradiation and hypoxic holding (H-H) and (d) hypoxic irradiation and aerobic holding (H-A). It has been found that the presence of oxygen is essential for recovery from the damage induced by both gamma rays and high linear energy transfer (LET) radiations. The extent of recovery was larger for gamma-induced damage than for damage induced by high LET radiation (alpha + 7Li) for the A-A condition. In the H-H condition, while only a slight recovery was seen for gamma-induced damage, it was totally absent for high LET damage. For the modality A-H, it was found that there is not recovery from the sparsely ionising gamma radiation-induced damage. The implications of these results for the treatment of malignant tumours by radiotherapy are briefly discussed. PMID:397200

  5. Nonlinear Ultrasonic Techniques to Monitor Radiation Damage in RPV and Internal Components

    SciTech Connect

    Jacobs, Laurence; Kim, Jin-Yeon; Qu, Jisnmin; Ramuhalli, Pradeep; Wall, Joe

    2015-11-02

    The objective of this research is to demonstrate that nonlinear ultrasonics (NLU) can be used to directly and quantitatively measure the remaining life in radiation damaged reactor pressure vessel (RPV) and internal components. Specific damage types to be monitored are irradiation embrittlement and irradiation assisted stress corrosion cracking (IASCC). Our vision is to develop a technique that allows operators to assess damage by making a limited number of NLU measurements in strategically selected critical reactor components during regularly scheduled outages. This measured data can then be used to determine the current condition of these key components, from which remaining useful life can be predicted. Methods to unambiguously characterize radiation related damage in reactor internals and RPVs remain elusive. NLU technology has demonstrated great potential to be used as a material sensor – a sensor that can continuously monitor a material’s damage state. The physical effect being monitored by NLU is the generation of higher harmonic frequencies in an initially monochromatic ultrasonic wave. The degree of nonlinearity is quantified with the acoustic nonlinearity parameter, β, which is an absolute, measurable material constant. Recent research has demonstrated that nonlinear ultrasound can be used to characterize material state and changes in microscale characteristics such as internal stress states, precipitate formation and dislocation densities. Radiation damage reduces the fracture toughness of RPV steels and internals, and can leave them susceptible to IASCC, which may in turn limit the lifetimes of some operating reactors. The ability to characterize radiation damage in the RPV and internals will enable nuclear operators to set operation time thresholds for vessels and prescribe and schedule replacement activities for core internals. Such a capability will allow a more clear definition of reactor safety margins. The research consists of three tasks: (1

  6. Radiation Damage Study in Natural Zircon Using Neutrons Irradiation

    SciTech Connect

    Lwin, Maung Tin Moe; Amin, Yusoff Mohd.; Kassim, Hasan Abu; Mohamed, Abdul Aziz; Karim, Julia Abdul

    2011-03-30

    Changes of atomic displacements in crystalline structure of natural zircon (ZrSiO{sub 4}) can be studied by using neutron irradiation on the surface of zircon and compared the data from XRD measurements before and after irradiation. The results of neutron irradiation on natural zircon using Pneumatic Transfer System (PTS) at PUSPATI TRIGA Research Reactor in the Malaysian Nuclear Agency are discussed in this work. The reactor produces maximum thermal power output of 1 MWatt and the neutron flux of up to 1x10{sup 13} ncm{sup -2}s{sup -1}. From serial decay processes of uranium and thorium radionuclides in zircon crystalline structure, the emission of alpha particles can produce damage in terms of atomic displacements in zircon. Hence, zircon has been extensively studied as a possible candidate for immobilization of fission products and actinides.

  7. Radiation Damage Study in Natural Zircon Using Neutrons Irradiation

    NASA Astrophysics Data System (ADS)

    Lwin, Maung Tin Moe; Amin, Yusoff Mohd.; Kassim, Hasan Abu; Mohamed, Abdul Aziz; Karim, Julia Abdul

    2011-03-01

    Changes of atomic displacements in crystalline structure of natural zircon (ZrSiO4) can be studied by using neutron irradiation on the surface of zircon and compared the data from XRD measurements before and after irradiation. The results of neutron irradiation on natural zircon using Pneumatic Transfer System (PTS) at PUSPATI TRIGA Research Reactor in the Malaysian Nuclear Agency are discussed in this work. The reactor produces maximum thermal power output of 1 MWatt and the neutron flux of up to 1×1013 ncm-2s-1. From serial decay processes of uranium and thorium radionuclides in zircon crystalline structure, the emission of alpha particles can produce damage in terms of atomic displacements in zircon. Hence, zircon has been extensively studied as a possible candidate for immobilization of fission products and actinides.

  8. Repair of ionizing radiation DNA base damage in ataxia-telangiectasia cells

    SciTech Connect

    Fornace, A.J. Jr.; Kinsella, T.J.; Dobson, P.P.; Mitchell, J.B.

    1986-04-01

    Micrococcus luteus endonuclease sensitive sites were measured by alkaline elution in normal human and ataxia-telangiectasia (AT) fibroblasts after ionizing radiation. Due to the sensitivity of this assay, repair of base damage after 3 to 6 kilorads has been measured after oxic or hypoxic radiation. With 5.5 kilorads of oxic radiation, more than 50% of the base damage was removed after 1.5 h of repair incubation in all cells, including exr+ and exr- AT cells, and approximately 75% was removed by 4 h. After 3 or 4.5 kilorads of hypoxic X-irradiation, repair was equivalent in normal and exr- AT cells. This study included three exr- AT strains which have been reported to be deficient in the removal of gamma-ray base damage at higher doses. Since these strains repaired ionizing radiation base damage normally at lower doses, which are more relevant to survival, it is concluded that the X-ray hypersensitivity of AT cells is probably not related to the repair of base damage.

  9. Autophagy confers DNA damage repair pathways to protect the hematopoietic system from nuclear radiation injury

    PubMed Central

    Lin, Weiwei; Yuan, Na; Wang, Zhen; Cao, Yan; Fang, Yixuan; Li, Xin; Xu, Fei; Song, Lin; Wang, Jian; Zhang, Han; Yan, Lili; Xu, Li; Zhang, Xiaoying; Zhang, Suping; Wang, Jianrong

    2015-01-01

    Autophagy is essentially a metabolic process, but its in vivo role in nuclear radioprotection remains unexplored. We observed that ex vivo autophagy activation reversed the proliferation inhibition, apoptosis, and DNA damage in irradiated hematopoietic cells. In vivo autophagy activation improved bone marrow cellularity following nuclear radiation exposure. In contrast, defective autophagy in the hematopoietic conditional mouse model worsened the hematopoietic injury, reactive oxygen species (ROS) accumulation and DNA damage caused by nuclear radiation exposure. Strikingly, in vivo defective autophagy caused an absence or reduction in regulatory proteins critical to both homologous recombination (HR) and non-homologous end joining (NHEJ) DNA damage repair pathways, as well as a failure to induce these proteins in response to nuclear radiation. In contrast, in vivo autophagy activation increased most of these proteins in hematopoietic cells. DNA damage assays confirmed the role of in vivo autophagy in the resolution of double-stranded DNA breaks in total bone marrow cells as well as bone marrow stem and progenitor cells upon whole body irradiation. Hence, autophagy protects the hematopoietic system against nuclear radiation injury by conferring and intensifying the HR and NHEJ DNA damage repair pathways and by removing ROS and inhibiting apoptosis. PMID:26197097

  10. Radiation damage studies for the SDC electromagnetic calorimeter

    NASA Astrophysics Data System (ADS)

    Fazely, A. R.; Gunasingha, R.; Imlay, R. L.; Khosravi, E. S.; Lim, Jit-Ning; Lyndon, C.; McMills, G.; McNeil, R. R.; Metcalf, W. J.; Courtney, J. C.; Tashakkori, R.; Vegara, B. J.

    1993-01-01

    We report the results from a year long study aimed at radiation resistance and optical performance of scintillator tile with green wave shifter fiber readout. A careful investigation of several rad-hard plastic scintillators from Bicron and Kuraray, studies indicate that for a specific rad-hard Bicron scintillator, it is possible to build a tile/fiber EM calorimeter that can operate in the design luminosity of SSC. This calorimeter with excellent optical response would only have a light loss of about 5% after being exposed to 1 Mrad.

  11. Radiation damage of heavy crystalline detector materials by 24 GeV protons

    NASA Astrophysics Data System (ADS)

    Barysevich, A.; Dormenev, V.; Fedorov, A.; Glaser, M.; Kobayashi, M.; Korjik, M.; Maas, F.; Mechinski, V.; Rusack, R.; Singovski, A.; Zoueyski, R.

    2013-02-01

    Samples of three heavy crystalline materials: PbWO4, Bi4Si3O12, and PbF2 were irradiated in a high-intensity 24 GeV proton beam at the CERN PS to fluencies of 3.8×1013 protons/cm2. The optical transmission radiation damage was measured and all crystals show a shift of the cutoff in the transmission spectrum that is not observed when the crystals are irradiated with γ radiation. This shift of the cutoff under proton irradiation seems to be a general property of the heavy crystalline materials. A mechanism for this proton-induced transmission damage is discussed.

  12. Prediction and measurement of radiation damage to CMOS devices on board spacecraft

    NASA Technical Reports Server (NTRS)

    Cliff, R. A.; Danchenko, V.; Stassinopoulos, E. G.; Sing, M.; Brucker, G. J.; Ohanian, R. S.

    1976-01-01

    The initial results obtained from the Complementary Metal Oxide Semiconductors Radiation Effects Measurement experiment are presented. Predictions of radiation damage to C-MOS devices are based on standard environment models and computational techniques. A comparison of the shifts in CMOS threshold potentials, that is, those measured in space to those obtained from the on the ground simulation experiment with Co 60, indicated that the measured space damage is greater than predicted by a factor of two for shields thicker than 100 mils (2.54 mm), but agrees well with predictions for the thinner shields.

  13. Amelioration of radiation-induced liver damage in partially hepatectomized rats by hepatocyte transplantation.

    PubMed

    Guha, C; Sharma, A; Gupta, S; Alfieri, A; Gorla, G R; Gagandeep, S; Sokhi, R; Roy-Chowdhury, N; Tanaka, K E; Vikram, B; Roy-Chowdhury, J

    1999-12-01

    Hepatic tumors often recur in the liver after surgical resection. Postoperative radiotherapy (RT) could improve survival, but curative RT may induce delayed life-threatening radiation-induced liver damage. Because RT inhibits liver regeneration, we hypothesized that unirradiated, transplanted hepatocytes would proliferate preferentially in a partially resected and irradiated liver, providing metabolic support. We subjected F344 rats to hepatic RT and partial hepatectomy with/without a single intrasplenic, syngeneic hepatocyte transplantation. Hepatocyte transplantation ameliorated radiation-induced liver damage and improved survival of rats receiving RT after partial hepatectomy. We further demonstrated that transplanted hepatocytes extensively repopulate and function in a heavily irradiated rat liver. PMID:10606225

  14. Reference data file for neutron spectrum adjustment and related radiation damage calculations

    SciTech Connect

    Zsolnay, E.M. ); Nolthenius, H.J.; Greenwood, L.R.; Szondi, E.J. )

    1990-08-01

    The REAL-88 interlaboratory exercise organized by IAEA resulted in a neutron metrology file. (NMF-90) comprising problem dependent data for benchmark neutron fields, furthermore, nuclear data and computer programs for neutron spectrum adjustment and radiation damage parameter calculations for the service life assessment of nuclear facilities. Calculation results of some experienced laboratories are also present. This paper describes and analyses the content of the neutron metrology file and outlines the most important problems and tasks to be solved in the field of radiation damage parameter calculations. 14 refs., 2 figs., 1 tab.

  15. Radiation damage in silicon due to albedo neutrons emitted from hadronic beam dumps (Fe and U)

    SciTech Connect

    Gabriel, T.A.; Bishop, B.L.

    1987-01-01

    Calculations have been carried out to determine the level of radiation damage that can be expected from albedo neutrons when 1- and 5-GeV negative pions are incident on iron and uranium beam dumps. The calculated damage data are presented in several ways including neutron fluence above 0.111 MeV, 1 MeV equivalent neutron fluence, damage energy deposition, and DPA or displacements per atom. Details are presented as to the method of calculation. 14 refs., 1 fig., 1 tab.

  16. Modification of radiation-induced oxidative damage in liposomal and microsomal membrane by eugenol

    NASA Astrophysics Data System (ADS)

    Pandey, B. N.; Lathika, K. M.; Mishra, K. P.

    2006-03-01

    Radiation-induced membrane oxidative damage, and their modification by eugenol, a natural antioxidant, was investigated in liposomes and microsomes. Liposomes prepared with DPH showed decrease in fluorescence after γ-irradiation, which was prevented significantly by eugenol and correlated with magnitude of oxidation of phospholipids. Presence of eugenol resulted in substantial inhibition in MDA formation in irradiated liposomes/microsomes, which was less effective when added after irradiation. Similarly, the increase in phospholipase C activity observed after irradiation in microsomes was inhibited in samples pre-treated with eugenol. Results suggest association of radio- oxidative membrane damage with alterations in signaling molecules, and eugenol significantly prevented these membrane damaging events.

  17. Modification of radiation damage in rat spinal cord by mitotane

    SciTech Connect

    Glicksman, A.S.; Bliven, S.F.; Leith, J.T.

    1982-07-01

    Modification of the paralytic response in rats after 6-MV photon irradiation of the spinal cord with either single or split exposures (two equal fractions given in a 24-hour period) by mitotane was investigated. Mitotane was administered as a suspension in physiologic saline (300 mg/kg/day) for either 5 days prior to or 5 days after irradiation. For rats receiving split doses of 6-MV photons, either the last two doses of mitotane were given 2 hours prior to each radiation fraction or mitotane was begun 2 hours after the second fraction and continued for 5 days. The data to 6 months after irradiation indicate that, in rats given mitotane for 5 days prior to single-dose photon irradiation, the paralytic response (as defined by the dose needed to produce paralysis in 50% of the irradiated groups of rats) was enhanced by a dose-enhancement factor (DEF) of 1.40. The DEF in the group of rats given mitotane after single doses of 6-MV photons was 1.15. In the split-dose irradiation experiments, the DEF for the groups of rats given mitotane prior to each radiation fraction was 1.36; while the DEF for the group of rats receiving mitotane beginning after the second fraction was 1.18. These data indicate that mitotane can potentiate the effects of 6-MV photon irradiation to the central nervous system, with mitotane administered prior to irradiation being the most effective sequence.

  18. Radiation damage in high voltage silicon solar cells

    NASA Technical Reports Server (NTRS)

    Weinberg, I.; Brandhorst, H., Jr.; Swartz, C. K.; Weizer, V. G.

    1980-01-01

    Three high open-circuit voltage cell designs based on 0.1 ohm-cm p-type silicon were irradiated with 1 MeV electrons and their performance determined to fluences as high as 10 to the 15th power/sq cm. Of the three cell designs, radiation induced degradation was greatest in the high-low emitter (HLE cell). The diffused and ion implanted cells degraded approximately equally but less than the HLE cell. Degradation was greatest in an HLE cell exposed to X-rays before electron irradiation. The cell regions controlling both short-circuit current and open-circuit voltage degradation were defined in all three cell types. An increase in front surface recombination velocity accompanied time dependent degradation of an HLE cell after X-irradiation. It was speculated that this was indirectly due to a decrease in positive charge at the silicon-oxide interface. Modifications aimed at reducing radiation induced degradation are proposed for all three cell types.

  19. Depletion layer recombination effects on the radiation damage hardness of gallium arsenide cells

    NASA Technical Reports Server (NTRS)

    Garlick, G. F. J.

    1985-01-01

    The significant effect of junction depletion layer recombination on the efficiency of windowed GaAs cells was demonstrated. The effect becomes more pronounced as radiation damage occurs. The depletion is considered for 1 MeV electron fluences up to 10 to the 16th power e/sq m. The cell modeling separates damage in emitter and base or buffer layers using different damage coefficients is reported. The lower coefficient for the emitter predicts less loss of performance at fluences greater than 10 to the 15th power e/sq cm. A method for obtaining information on junction recombination effects as damage proceeds is described; this enables a more complete diagnosis of damage to be made.

  20. Radiation-induced renal damage: the effects of hyperfractionation. [Mice

    SciTech Connect

    Stewart, F.A.; Soranson, J.A.; Alpen, E.L.; Williams, M.V.; Denekamp, J.

    1984-05-01

    The response of mouse kidneys to multifraction irradiation was assessed using three nondestructive functional end points. A series of schedules was investigated giving 1, 2, 4, 8, 16, 32, or 64 equal X-ray doses, using doses per fraction in the range of 0.9 to 16 Gy. The overall treatment time was kept constant at 3 weeks. Kidney function was assessed from 19 to 48 weeks after irradiation by measuring changes in isotope clearance, urine output, and hematocrit. All three assays yielded steep dose-effect curves from which the repair capacity of kidney could be estimated by comparing the isoeffective doses in different schedules. There was a marked influence of fractionation, with increasing dose being required to achieve the same level of damage for increasing fraction number, even between 32 and 64 fractions. The data are well fitted by a linear quadratic dose-response equation, and analysis of the data would suggest that hyperfractionation, using extremely small X-ray doses per fraction, would spare kidneys relative to tumors and acutely responding tissues.

  1. Radiation damage study using small-angle neutron scattering

    NASA Astrophysics Data System (ADS)

    Rétfalvi, E.; Török, Gy; Rosta, L.

    2000-03-01

    Nuclear radiation provides important changes in the microstructure of metallic components of nuclear power plant and research reactors, influencing their mechanical properties. The investigation of this problem has primary interest for the safety and life-time of such nuclear installations. For the characterization of this kind of nanostructures small angle neutron scattering technique is a very useful tool. We have carried out experiments on samples of irradiated reactor vessel material and welded components of VVER-440-type reactors on the SANS instrument at the Budapest Research Reactor. In our measurements irradiated as well as non-irradiated samples were compared and magnetic field was applied for viewing the magnetic structure effects of the materials. A clear modification of the structure due to irradiation was obtained. Our data were analyzed by the ITP92 code, the inverse Fourier transform program of O. Glatter [1].

  2. Ion-counting nanodosimetry: a new method for assessing radiation damage to DNA

    NASA Astrophysics Data System (ADS)

    Shchemelinin, S.; Garty, G.; Breskin, A.; Chechik, R.; Schulte, R. W. M.

    2002-01-01

    A novel nanodosimeter is described, based on ion counting. It provides precise model-evaluation of radiation-induced ionization patterns in small condensed-matter volumes of nanometric size. The nanodosimeter consists of a millimetric, low-pressure, wall-less gas cell, serving as an expanded model of a nanometric condensed-matter volume. The method can also be employed for the assessment of radiation damage to advanced nanoelectronics.

  3. Human umbilical cord mesenchymal stem cells alleviate nasal mucosa radiation damage in a guinea pig model.

    PubMed

    Duan, Hong-Gang; Ji, Fang; Zheng, Chun-Quan; Wang, Chun-Hua; Li, Jing

    2015-02-01

    Nasal complications after radiotherapy severely affect the quality of life of nasopharyngeal carcinoma patients, and there is a compelling need to find novel therapies for nasal epithelial cell radiation damage. Therefore, we investigated the therapeutic effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) in guinea pig model of nasal mucosa radiation damage and explored its therapeutic mechanism. Cultured hUC-MSCs were injected intravenously immediately after radiation in the nasal mucosa-radiation-damage guinea pig model. Migration of hUC-MSCs into the nasal mucosa and the potential for differentiation into nasal epithelial cells were evaluated by immunofluorescence. The therapeutic effects of hUC-MSCs were evaluated by mucus clearance time (MCT), degree of nasal mucosa edema, and the nasal mucosa cilia form and coverage ratio. Results indicate that the hUC-MSCs migrated to the nasal mucosa lamina propria and did not differentiate into nasal epithelial cells in this model. The MCT and degree of mucosal edema were improved at 1 week and 1 month after radiation, respectively, but no difference was found at 3 months and 6 months after radiation. The nasal mucosa cilia form and coverage ratio was not improved 6 months after radiation. Thus, hUC-MSCs can migrate to the nasal mucosa lamina propria and improve MCT and mucosa edema within a short time period, but these cells are unable to differentiate into nasal epithelial cells and improve nasal epithelial regeneration in the nasal mucosa radiation damage guinea pig model. PMID:25209829

  4. Spectromicroscopy of Polymers: Comparison of Radiation Damage with Electron and Photon Core Excitation Spectroscopy Techniques

    NASA Astrophysics Data System (ADS)

    Ade, H.; Smith, A. P.; Rightor, E. G.; Hitchcock, A. P.; Urquhart, S.; Leapman, R.

    1997-03-01

    Core excitation microspectroscopy has become a powerful tool for the characterization of polymeric materials due to its sensitivity to chemical functionality. However, the excitations utilized in electron energy loss spectroscopy performed in a scanning transmission electron microscope (TEM-EELS) and near edge x-ray absorption fine structure (NEXAFS) spectroscopy can introduce radiation damage and chemically modify the sample. In order to understand the radiation damage associated with TEM-EELS and NEXAFS spectroscopy we have studied the radiation damage of the common polymer poly(ethylene terephthalate) (PET) as exhibited by changes in the acquired C K-edge excitation spectra. By fitting gaussian functions to the spectral intensity changes as a function of dose, we have determined the critical radiation dose of PET for both NEXAFS spectroscopy and TEM-EELS under typical operating conditions. This critical radiation dose for TEM-EELS is found to be 1.7 ± 0.2 x 10^8 grey (1.7 ± 0.2 x 10^4 Mrad) compared to a critical radiation dose for NEXAFS spectroscopy of 1.4 ± 0.7 x 10^9 grey (1.4 ± 0.7 x 10^5 Mrad). By considering the G factors of the two techniques and the critical radiation dose, a rule of thumb was derived that indicates that with typical present operating conditions, NEXAFS spectroscopy can analyze areas 500 times smaller than TEM-EELS given the same amount of radiation damage. Work supported by: NSF Young Investigator Award (DMR-9458060) and Dow Chemical

  5. Concurrent Transient Activation of Wnt/{beta}-Catenin Pathway Prevents Radiation Damage to Salivary Glands

    SciTech Connect

    Hai Bo; Yang Zhenhua; Shangguan Lei; Zhao Yanqiu; Boyer, Arthur; Liu, Fei

    2012-05-01

    Purpose: Many head and neck cancer survivors treated with radiotherapy suffer from permanent impairment of their salivary gland function, for which few effective prevention or treatment options are available. This study explored the potential of transient activation of Wnt/{beta}-catenin signaling in preventing radiation damage to salivary glands in a preclinical model. Methods and Materials: Wnt reporter transgenic mice were exposed to 15 Gy single-dose radiation in the head and neck area to evaluate the effects of radiation on Wnt activity in salivary glands. Transient Wnt1 overexpression in basal epithelia was induced in inducible Wnt1 transgenic mice before together with, after, or without local radiation, and then saliva flow rate, histology, apoptosis, proliferation, stem cell activity, and mRNA expression were evaluated. Results: Radiation damage did not significantly affect activity of Wnt/{beta}-catenin pathway as physical damage did. Transient expression of Wnt1 in basal epithelia significantly activated the Wnt/{beta}-catenin pathway in submandibular glands of male mice but not in those of females. Concurrent transient activation of the Wnt pathway prevented chronic salivary gland dysfunction following radiation by suppressing apoptosis and preserving functional salivary stem/progenitor cells. In contrast, Wnt activation 3 days before or after irradiation did not show significant beneficial effects, mainly due to failure to inhibit acute apoptosis after radiation. Excessive Wnt activation before radiation failed to inhibit apoptosis, likely due to extensive induction of mitosis and up-regulation of proapoptosis gene PUMA while that after radiation might miss the critical treatment window. Conclusion: These results suggest that concurrent transient activation of the Wnt/{beta}-catenin pathway could prevent radiation-induced salivary gland dysfunction.

  6. Optimization of radiation damage to proteins using X-ray nanofocusing optics

    NASA Astrophysics Data System (ADS)

    Boularaoui, Selwa; Evans-Lutterodt, K.; Lee, S.; Isakovic, A. F.

    2013-03-01

    The need to understand protein structure and perform treatment lead to the use of X-ray and particle-based radiation. Since the use of such radiation has undesirable side effects, mostly through the damage to proteins, it is important to continuously work on decreasing radiation damage. We outline the proposal to use the kinoform refractive optics to focus X-rays on the nanoscale to minimize the radiation damage to protein crystals under study. These optics devices are nanofabricated from low-Z elements (silicon, diamond) and can be used at synchrotron X-ray radiation facilities. We discuss the automated setup that performs nanopositioning of the nanofocusing element, and collects the chemical and structural protein solution under study. We offer simple mathematical models in irradiation and in treatment that help optimize the radiation parameters. This work is supported in part by Khalifa University IRF-Level 1 Fund. The work at BNL-NSLS is supported through US DOE, Office of Basic Energy Sciences.

  7. Near infrared radiation damage mechanism in the lens

    NASA Astrophysics Data System (ADS)

    Söderberg, Per G.; Talebizadeh, Nooshin; Galichanin, Konstantin; Kronschläger, Martin; Schulmeister, Karl; Yu, Zhaohua

    2015-03-01

    The current data strongly indicates that there is no photochemical effect of in vivo exposure to 1090 nm near IRR radiation within the pupil. Four groups of 20 Sprague-Dawley rats were unilaterally exposed in vivo to 96 W·cm-2 centered inside the pupil for 10, 18, 33 and 60 min, respectively depending on group belonging. This resulted in radiant exposure doses of 57, 103, 198 and 344 kJ·cm-2. Temperature evolution at the limbus during the exposure and difference of intensity of forward light scattering between the exposed and the contralateral not exposed eye was measured at 1 week after exposure. The temperature at the limbus was found to increase exponentially towards an asymptote with an asymptote temperature of around 7 °C and a time constant (1/k) of around 15 s. No increase of light scattering was found despite that the cumulated radiant exposure dose was [80;250] times the threshold for photochemically induced cataract suggested by previous empirical data. It is concluded that at 1090 nm near IRR there is no photochemical effect.

  8. TEM study of radiation damage and annealing of neutron irradiated zirconolite

    SciTech Connect

    Lumpkin, G.R.; Smith, K.L.; Blake, R.G.

    1996-08-01

    Neutron irradiation was used to simulate alpha-decay damage in zirconolite, resulting in a transformation from the crystalline to the amorphous state at doses of 4--25 {times} 10{sup 19} n/cm{sup 2} (E {ge} 1 MeV). With increasing dose, the radiation damage microstructures resemble damage caused by: (1) alpha-decay of {sup 232}Th and {sup 238}U in natural zirconolites, (2) alpha-decay of {sup 238}Pu or {sup 244}Cm in synthetic samples, and (3) collision cascades in samples irradiated with heavy ions. Heavily damaged zirconolite recovers to a defect fluorite phase on annealing at temperatures up to 1,000 C. The main stage of structural recovery was found to occur at temperatures of 600--800 C. The microstructures after heating depend on the initial level of damage: zirconolite grains with low to moderate levels of damage anneal to imperfect single crystals, whereas heavily damaged grains recrystallize to a polycrystalline microstructure. Complications encountered in this work include the production of fission tracks (due to trace amounts of U) and a non-uniform distribution of damage at higher dose levels (possibly due to electron beam heating).

  9. Can radiation damage to protein crystals be reduced using small-molecule compounds?

    PubMed Central

    Kmetko, Jan; Warkentin, Matthew; Englich, Ulrich; Thorne, Robert E.

    2011-01-01

    Recent studies have defined a data-collection protocol and a metric that provide a robust measure of global radiation damage to protein crystals. Using this protocol and metric, 19 small-molecule compounds (introduced either by cocrystalliz­ation or soaking) were evaluated for their ability to protect lysozyme crystals from radiation damage. The compounds were selected based upon their ability to interact with radiolytic products (e.g. hydrated electrons, hydrogen, hydroxyl and perhydroxyl radicals) and/or their efficacy in protecting biological molecules from radiation damage in dilute aqueous solutions. At room temperature, 12 compounds had no effect and six had a sensitizing effect on global damage. Only one compound, sodium nitrate, appeared to extend crystal lifetimes, but not in all proteins and only by a factor of two or less. No compound provided protection at T = 100 K. Scavengers are ineffective in protecting protein crystals from global damage because a large fraction of primary X-ray-induced excitations are generated in and/or directly attack the protein and because the ratio of scavenger molecules to protein molecules is too small to provide appreciable competitive protection. The same reactivity that makes some scavengers effective radioprotectors in protein solutions may explain their sensitizing effect in the protein-dense environment of a crystal. A more productive focus for future efforts may be to identify and eliminate sensitizing compounds from crystallization solutions. PMID:21931220

  10. 3D imaging of radiation damage in silicon sensor and spatial mapping of charge collection efficiency

    NASA Astrophysics Data System (ADS)

    Jakubek, M.; Jakubek, J.; Zemlicka, J.; Platkevic, M.; Havranek, V.; Semian, V.

    2013-03-01

    Radiation damage in semiconductor sensors alters the response and degrades the performance of many devices ultimately limiting their stability and lifetime. In semiconductor radiation detectors the homogeneity of charge collection becomes distorted while decreasing the overall detection efficiency. Moreover the damage can significantly increase the detector noise and degrade other electrical properties such as leakage current. In this work we present a novel method for 3D mapping of the semiconductor radiation sensor volume allowing displaying the three dimensional distribution of detector properties such as charge collection efficiency and charge diffusion rate. This technique can visualize the spatially localized changes of local detector performance after radiation damage. Sensors used were 300 μm and 1000 μm thick silicon bump-bonded to a Timepix readout chip which serves as an imaging multichannel microprobe (256 × 256 square pixels with pitch of 55 μm, i.e. all together 65 thousand channels). Per pixel energy sensitivity of the Timepix chip allows to evaluate the local charge collection efficiency and also the charge diffusion rate. In this work we implement an X-ray line scanning technique for systematic evaluation of changes in the performance of a silicon sensor intentionally damaged by energetic protons.

  11. Variable-Temperature Cryostat For Radiation-Damage Testing Of Germanium Detectors

    NASA Technical Reports Server (NTRS)

    Floyd, Samuel R.; Puc, Bernard P.

    1992-01-01

    Variable-temperature cryostats developed to study radiation damage to, and annealing of, germanium gamma-ray detectors. Two styles: one accommodates large single detector and one accommodates two medium-sized detectors. New cryostats allow complete testing of large-volume germanium gamma-ray detectors without breaking cryostat vacuum and removing detectors for annealing.

  12. TGF-.beta. antagonists as mitigators of radiation-induced tissue damage

    DOEpatents

    Barcellos-Hoff, Mary H.

    1997-01-01

    A method for treating tissue damage caused by radiation is described by use of a TGF-.beta. antagonist, such as an anti-TGF-.beta. antibody or a TGF-.beta. latency associated protein. It is administered not more than a week after exposure, and is particularly useful in mitigating the side effects of breast cancer therapy.

  13. Geraniin down regulates gamma radiation-induced apoptosis by suppressing DNA damage.

    PubMed

    Bing, So Jin; Ha, Danbee; Kim, Min Ju; Park, Eunjin; Ahn, Ginnae; Kim, Dae Seung; Ko, Ryeo Kyeong; Park, Jae Woo; Lee, Nam Ho; Jee, Youngheun

    2013-07-01

    Gamma ray irradiation triggers DNA damage and apoptosis of proliferating stem cells and peripheral immune cells, resulting in the destruction of intestinal crypts and lymphoid system. Geraniin is a natural compound extracts from an aquatic plant Nymphaea tetragona and possesses good antioxidant property. In this study, we demonstrate that geraniin rescues radiosensitive splenocytes and jejunal crypt cells from radiation-induced DNA damage and apoptosis. Isolated splenocytes from C57BL/6 mice treated with geraniin were protected against radiation injury of 2 Gy irradiation through the enhancement of the proliferation and attenuation of DNA damage. Also, geraniin inhibited apoptosis in radiosensitive splenocytes by reducing the expression level and immunoreactivity of proapoptotic p53 and Bax and increasing those of anti-apoptotic Bcl-2. In mice exposed to radiation, geraniin treatment protected splenocytes and intestinal crypt cells from radiation-induced cell death. Our results suggest that geraniin presents radioprotective effects by regulating DNA damage on splenocytes, exerting immunostimulatory capacities and inhibiting apoptosis of radiosensitive immune cells and jejunal crypt cells. Therefore, geraniin can be a radioprotective agent against γ-irradiation exposure. PMID:23541438

  14. TGF-{beta} antagonists as mitigators of radiation-induced tissue damage

    DOEpatents

    Barcellos-Hoff, M.H.

    1997-04-01

    A method for treating tissue damage caused by radiation is described by use of a TGF-{beta} antagonist, such as an anti-TGF-{beta} antibody or a TGF-{beta} latency associated protein. It is administered not more than a week after exposure, and is particularly useful in mitigating the side effects of breast cancer therapy.

  15. Measurement of high-voltage and radiation-damage limitations to advanced solar array performance

    NASA Technical Reports Server (NTRS)

    Guidice, D. A.; Severance, P. S.; Keinhardt, K. C.

    1991-01-01

    A description is given of the reconfigured Photovoltaic Array Space Power (PASP) Plus experiment: its objectives, solar-array complement, and diagnostic sensors. Results from a successful spaceflight will lead to a better understanding of high-voltage and radiation-damage limitations in the operation of new-technology solar arrays.

  16. Ultraviolet radiation-specific DNA damage and embryonic viability in sea urchins from Kasitsna Bay, Alaska

    SciTech Connect

    Theodorakis, C.; Anderson, S.; Shugart, L.R.

    1995-12-31

    Ripe ova and sperm were obtained from Green Sea Urchins (Strongvlocentrotus drochbachiensis) collected from Kasitsna Bay, Alaska, and ova were fertilized in vitro. Embryos were immediately placed in plastic bags secured to floating racks deployed in the bay. The bags were suspended just below the surface of the water and at 1 and 2 meter depths for up to 120 hours. Bags were either left uncovered, covered with Mylar plastic (which blocks out UV-B but not UV-A radiations), or covered with dark plastic. The number of damaged DNA sites was determined by digesting the DNA with enzymes isolated from the bacterium Micrococcus luteus which cleave the DNA at damaged sites. It was found that DNA damage was present in a dose-dependent fashion with the amount of damage in embryos from the uncovered bags > Mylar covered bags > dark covered bags. No dimers were detected from embryos at 1 or 2 m. depths. Also, the number of damaged sites varied from day to day. Finally, the number of damaged sites was positively correlated with percent abnormal embryos in each bag. The results are discussed with relation to monitoring UV-B effects and ecological consequences of enhanced UV-B radiation.

  17. Determining the Radiation Damage Effect on Glovebox Glove Material.

    SciTech Connect

    Cournoyer, M. E.; Balkey, J. J.; Andrade, R.M.

    2005-01-01

    The Nuclear Material Technology (NMT) Division has the largest inventory of glove box gloves at Los Alamos National Laboratory. The minimization of unplanned breaches in the glovebox, e.g., glove failures, is a primary concern in the daily operations in NMT Division facilities, including the Plutonium Facility (PF-4) at TA-55 and Chemical and Metallurgy Research (CMR) Facility. Glovebox gloves in these facilities are exposed to elevated temperatures and exceptionally aggressive radiation environments (particulate {sup 239}Pu and {sup 238}Pu). Predictive models are needed to estimate glovebox glove service lifetimes, i.e. change-out intervals. Towards this aim aging studies have been initiated that correlate changes in mechanical (physical) properties with degradation chemistry. This present work derives glovebox glove change intervals based on previously reported mechanical data of thermally aged hypalon glove samples. Specifications for 30 mil tri-layered hypalon/lead glovebox gloves (TLH) and 15 mil hypalon gloves (HYP) have already been established. The relevant mechanical properties are shown on Table 1. Tensile strength is defined as the maximum load applied in breaking a tensile test piece divided by the original cross-sectional area of the test piece (Also termed maximum stress and ultimate tensile stress). Ultimate elongation is the elongation at time of rupture (Also termed maximum strain). The specification for the tensile test and ultimate elongation are the minimum acceptable values. In addition, the ultimate elongation must not vary 20% from the original value. In order to establish a service lifetimes for glovebox gloves in a thermal environment, the mechanical properties of glovebox glove materials were studied.

  18. Radiation-damage-induced phasing: a case study using UV irradiation with light-emitting diodes.

    PubMed

    de Sanctis, Daniele; Zubieta, Chloe; Felisaz, Franck; Caserotto, Hugo; Nanao, Max H

    2016-03-01

    Exposure to X-rays, high-intensity visible light or ultraviolet radiation results in alterations to protein structure such as the breakage of disulfide bonds, the loss of electron density at electron-rich centres and the movement of side chains. These specific changes can be exploited in order to obtain phase information. Here, a case study using insulin to illustrate each step of the radiation-damage-induced phasing (RIP) method is presented. Unlike a traditional X-ray-induced damage step, specific damage is introduced via ultraviolet light-emitting diodes (UV-LEDs). In contrast to UV lasers, UV-LEDs have the advantages of small size, low cost and relative ease of use. PMID:26960126

  19. Spontaneous perseverative turning in rats with radiation-induced hippocampal damage

    SciTech Connect

    Mickley, G.A.; Ferguson, J.L.; Nemeth, T.J.; Mulvihill, M.A.; Alderks, C.E. )

    1989-08-01

    This study found a new behavioral correlate of lesions specific to the dentate granule cell layer of the hippocampus: spontaneous perseverative turning. Irradiation of a portion of the neonatal rat cerebral hemispheres produced hypoplasia of the granule cell layer of the hippocampal dentate gyrus while sparing the rest of the brain. Radiation-induced damage to the hippocampal formation caused rats placed in bowls to spontaneously turn in long, slow bouts without reversals. Irradiated subjects also exhibited other behaviors characteristic of hippocampal damage (e.g., perseveration in spontaneous exploration of the arms of a T-maze, retarded acquisition of a passive avoidance task, and increased horizontal locomotion). These data extend previously reported behavioral correlates of fascia dentata lesions and suggest the usefulness of a bout analysis of spontaneous bowl turning as a measure of nondiscrete-trial spontaneous alternation and a sensitive additional indicator of radiation-induced hippocampal damage.

  20. Study of terahertz-radiation-induced DNA damage in human blood leukocytes

    SciTech Connect

    Angeluts, A A; Esaulkov, M N; Kosareva, O G; Solyankin, P M; Shkurinov, A P; Gapeyev, A B; Pashovkin, T N; Matyunin, S N; Nazarov, M M; Cherkasova, O P

    2014-03-28

    We have carried out the studies aimed at assessing the effect of terahertz radiation on DNA molecules in human blood leukocytes. Genotoxic testing of terahertz radiation was performed in three different oscillation regimes, the blood leukocytes from healthy donors being irradiated for 20 minutes with the mean intensity of 8 – 200 μW cm{sup -2} within the frequency range of 0.1 – 6.5 THz. Using the comet assay it is shown that in the selected regimes such radiation does not induce a direct DNA damage in viable human blood leukocytes. (biophotonics)

  1. Study of terahertz-radiation-induced DNA damage in human blood leukocytes

    NASA Astrophysics Data System (ADS)

    Angeluts, A. A.; Gapeyev, A. B.; Esaulkov, M. N.; Kosareva, O. G.; Matyunin, S. N.; Nazarov, M. M.; Pashovkin, T. N.; Solyankin, P. M.; Cherkasova, O. P.; Shkurinov, A. P.

    2014-03-01

    We have carried out the studies aimed at assessing the effect of terahertz radiation on DNA molecules in human blood leukocytes. Genotoxic testing of terahertz radiation was performed in three different oscillation regimes, the blood leukocytes from healthy donors being irradiated for 20 minutes with the mean intensity of 8 - 200 μW cm-2 within the frequency range of 0.1 - 6.5 THz. Using the comet assay it is shown that in the selected regimes such radiation does not induce a direct DNA damage in viable human blood leukocytes.

  2. Can radiation damage to protein crystals be reduced using small-molecule compounds?

    SciTech Connect

    Kmetko, Jan; Warkentin, Matthew; Englich, Ulrich; Thorne, Robert E.

    2011-10-01

    Free-radical scavengers that are known to be effective protectors of proteins in solution are found to increase global radiation damage to protein crystals. Protective mechanisms may become deleterious in the protein-dense environment of a crystal. Recent studies have defined a data-collection protocol and a metric that provide a robust measure of global radiation damage to protein crystals. Using this protocol and metric, 19 small-molecule compounds (introduced either by cocrystallization or soaking) were evaluated for their ability to protect lysozyme crystals from radiation damage. The compounds were selected based upon their ability to interact with radiolytic products (e.g. hydrated electrons, hydrogen, hydroxyl and perhydroxyl radicals) and/or their efficacy in protecting biological molecules from radiation damage in dilute aqueous solutions. At room temperature, 12 compounds had no effect and six had a sensitizing effect on global damage. Only one compound, sodium nitrate, appeared to extend crystal lifetimes, but not in all proteins and only by a factor of two or less. No compound provided protection at T = 100 K. Scavengers are ineffective in protecting protein crystals from global damage because a large fraction of primary X-ray-induced excitations are generated in and/or directly attack the protein and because the ratio of scavenger molecules to protein molecules is too small to provide appreciable competitive protection. The same reactivity that makes some scavengers effective radioprotectors in protein solutions may explain their sensitizing effect in the protein-dense environment of a crystal. A more productive focus for future efforts may be to identify and eliminate sensitizing compounds from crystallization solutions.

  3. Protecting the radiation-damaged skin from friction: a mini review

    SciTech Connect

    Herst, Patries M

    2014-06-15

    Radiation-induced skin reactions are an unavoidable side effect of external beam radiation therapy, particularly in areas prone to friction and excess moisture such as the axilla, head and neck region, perineum and skin folds. Clinical studies investigating interventions for preventing or managing these reactions have largely focussed on formulations with moisturising, anti-inflammatory, anti-microbial and wound healing properties. However, none of these interventions has emerged as a consistent candidate for best practice. Much less emphasis has been placed on evaluating ways to protect the radiation-damaged skin from friction and excess moisture. This mini review analyses the clinical evidence for barrier products that form a protective layer by adhering very closely to the skin folds and do not cause further trauma to the radiation-damaged skin upon removal. A database search identified only two types of barrier products that fitted these criteria and these were tested in two case series and six controlled clinical trials. Friction protection was most effective when the interventions were used from the start of treatment and continued for several weeks after completion of treatment. Soft silicone dressings (Mepilex Lite and Mepitel Film) and Cavilon No Sting Barrier Film, but not Cavilon Moisturizing Barrier Cream, decreased skin reaction severity, most likely due to differences in formulation and skin build-up properties. It seems that prophylactic use of friction protection of areas at risk could be a worthwhile addition to routine care of radiation-damaged skin.

  4. Recovery of Deinococcus radiodurans from radiation damage was enhanced under microgravity.

    PubMed

    Kobayashi, Y; Kikuchi, M; Nagaoka, S; Watanabe, H

    1996-09-01

    Effect of microgravity on recovery of bacterial cells from radiation damage was examined on the IML-2 mission in 1994 using extremely radioresistant bacterium Deinococcus radiodurans. The cells were lyophilized and exposed to 60Co gamma-rays with doses 2 to 12 kGy before the space flight. At the end of the mission, the cells were mixed on board with liquid nutrient medium to allow the cells to start recovery process from the radiation damage. Afterwards the cells were stored at 4 degrees C until landing. The influence of cosmic radiation was negligible, because total absorbed dose of space radiation measured during the mission was less than 2 mGy and this bacterium does not decrease its viability after both gamma-rays and high-LET heavy charged particles irradiation with doses up to 5 kGy. The survival of the cells incubated in space increased significantly compared with the ground controls, suggesting that the recovery of this bacterium from radiation damage was enhanced under microgravity. PMID:11785538

  5. Protecting the radiation-damaged skin from friction: a mini review

    PubMed Central

    Herst, Patries M

    2014-01-01

    Radiation-induced skin reactions are an unavoidable side effect of external beam radiation therapy, particularly in areas prone to friction and excess moisture such as the axilla, head and neck region, perineum and skin folds. Clinical studies investigating interventions for preventing or managing these reactions have largely focussed on formulations with moisturising, anti-inflammatory, anti-microbial and wound healing properties. However, none of these interventions has emerged as a consistent candidate for best practice. Much less emphasis has been placed on evaluating ways to protect the radiation-damaged skin from friction and excess moisture. This mini review analyses the clinical evidence for barrier products that form a protective layer by adhering very closely to the skin folds and do not cause further trauma to the radiation-damaged skin upon removal. A database search identified only two types of barrier products that fitted these criteria and these were tested in two case series and six controlled clinical trials. Friction protection was most effective when the interventions were used from the start of treatment and continued for several weeks after completion of treatment. Soft silicone dressings (Mepilex Lite and Mepitel Film) and Cavilon No Sting Barrier Film, but not Cavilon Moisturizing Barrier Cream, decreased skin reaction severity, most likely due to differences in formulation and skin build-up properties. It seems that prophylactic use of friction protection of areas at risk could be a worthwhile addition to routine care of radiation-damaged skin. PMID:26229646

  6. Protecting the radiation-damaged skin from friction: a mini review.

    PubMed

    Herst, Patries M

    2014-06-01

    Radiation-induced skin reactions are an unavoidable side effect of external beam radiation therapy, particularly in areas prone to friction and excess moisture such as the axilla, head and neck region, perineum and skin folds. Clinical studies investigating interventions for preventing or managing these reactions have largely focussed on formulations with moisturising, anti-inflammatory, anti-microbial and wound healing properties. However, none of these interventions has emerged as a consistent candidate for best practice. Much less emphasis has been placed on evaluating ways to protect the radiation-damaged skin from friction and excess moisture. This mini review analyses the clinical evidence for barrier products that form a protective layer by adhering very closely to the skin folds and do not cause further trauma to the radiation-damaged skin upon removal. A database search identified only two types of barrier products that fitted these criteria and these were tested in two case series and six controlled clinical trials. Friction protection was most effective when the interventions were used from the start of treatment and continued for several weeks after completion of treatment. Soft silicone dressings (Mepilex Lite and Mepitel Film) and Cavilon No Sting Barrier Film, but not Cavilon Moisturizing Barrier Cream, decreased skin reaction severity, most likely due to differences in formulation and skin build-up properties. It seems that prophylactic use of friction protection of areas at risk could be a worthwhile addition to routine care of radiation-damaged skin. PMID:26229646

  7. Clustered DNA damages induced in isolated DNA and in human cells by low doses of ionizing radiation

    NASA Technical Reports Server (NTRS)

    Sutherland, B. M.; Bennett, P. V.; Sidorkina, O.; Laval, J.; Lowenstein, D. I. (Principal Investigator)

    2000-01-01

    Clustered DNA damages-two or more closely spaced damages (strand breaks, abasic sites, or oxidized bases) on opposing strands-are suspects as critical lesions producing lethal and mutagenic effects of ionizing radiation. However, as a result of the lack of methods for measuring damage clusters induced by ionizing radiation in genomic DNA, neither the frequencies of their production by physiological doses of radiation, nor their repairability, nor their biological effects are known. On the basis of methods that we developed for quantitating damages in large DNAs, we have devised and validated a way of measuring ionizing radiation-induced clustered lesions in genomic DNA, including DNA from human cells. DNA is treated with an endonuclease that induces a single-strand cleavage at an oxidized base or abasic site. If there are two closely spaced damages on opposing strands, such cleavage will reduce the size of the DNA on a nondenaturing gel. We show that ionizing radiation does induce clustered DNA damages containing abasic sites, oxidized purines, or oxidized pyrimidines. Further, the frequency of each of these cluster classes is comparable to that of frank double-strand breaks; among all complex damages induced by ionizing radiation, double-strand breaks are only about 20%, with other clustered damage constituting some 80%. We also show that even low doses (0.1-1 Gy) of high linear energy transfer ionizing radiation induce clustered damages in human cells.

  8. Non-randomized mtDNA damage after ionizing radiation via charge transport

    NASA Astrophysics Data System (ADS)

    Zhou, Xin; Liu, Xinguo; Zhang, Xin; Zhou, Rong; He, Yang; Li, Qiang; Wang, Zhenhua; Zhang, Hong

    2012-10-01

    Although it is well known that there are mutation hot spots in mtDNA, whether there are damage hot spots remain elusive. In this study, the regional DNA damage of mitochondrial genome after ionizing radiation was determined by real-time quantitative PCR. The mtDNA damage level was found to be dose-dependent and regional unequal. The control region was the most susceptible region to oxidative damage. GGG, as an typical hole trap during charge transport, was found to be disproportionally enriched in the control region. A total of 107 vertebrate mitochondrial genomes were then analyzed to testify whether the GGG enrichment in control region was evolutionary conserved. Surprisingly, the triple G enrichment can be observed in most of the homeothermal animals, while the majority of heterothermic animals showed no triple G enrichment. These results indicated that the triple G enrichment in control region was related to the mitochondrial metabolism during evolution.

  9. Image dissector photocathode solar damage test program. [solar radiation shielding using a fast optical lens

    NASA Technical Reports Server (NTRS)

    Smith, R. A.

    1977-01-01

    Image dissector sensors of the same type which will be used in the NASA shuttle star tracker were used in a series of tests directed towards obtaining solar radiation/time damage criteria. Data were evaluated to determine the predicted level of operability of the star tracker if tube damage became a reality. During the test series a technique for reducing the solar damage effect was conceived and verified. The damage concepts are outlined and the test methods and data obtained which were used for verification of the technique's feasibility are presented. The ability to operate an image dissector sensor with the solar image focussed on the photocathode by a fast optical lens under certain conditions is feasible and the elimination of a mechanical protection device is possible.

  10. Radiation damage in front and back illuminated high resistivity silicon solar cells

    NASA Technical Reports Server (NTRS)

    Weinberg, I.; Goradia, C. P.; Swartz, C. K.; Brandhorst, H. W., Jr.

    1982-01-01

    Radiation induced degradation, in front and back illuminated 84 and 1250 ohm-cm n+pp+ silicon solar cells, was determined and cell performance interpreted using calculated optically injected charge distributions and cell voltage components. The 84 ohm-cm cell degraded less when illuminated from the front or n+ side compared to that when illuminated from the back or p+ side. On the other hand, the 1250 ohm-cm cell degraded less when back illuminated. It is concluded that, in addition to the usual mechanisms leading to decreased collection efficiencies, loss of conductivity modulation is a major cause of radiation damage in high resistivity silicon solar cells. These results suggest that radiation damage to high resistivity n+pp+ cells can be decreased by increasing cell collection efficiency and illuminating the cells from the p+ side.

  11. Prediction and measurement of radiation damage to CMOS devices on board spacecraft

    NASA Technical Reports Server (NTRS)

    Cliff, R. A.; Danchenko, V.; Stassinopoulos, E. G.; Sing, M.; Brucker, G. J.; Ohanian, R. S.

    1976-01-01

    The CMOS Radiation Effects Measurement (CREM) experiment is presently being flown on the Explorer-55. The purpose of the experiment is to evaluate device performance in the actual space radiation environment and to correlate the respective measurements to on-the-ground laboratory irradiation results. The experiment contains an assembly of C-MOS and P-MOS devices shielded in front by flat slabs of aluminum and by a practically infinite shield in the back. Predictions of radiation damage to C-MOS devices are based on standard environment models and computational techniques. A comparison of the shifts in CMOS threshold potentials, that is, those measured in space to those obtained from the on-the-ground simulation experiment with Co-60, indicates that the measured space damage is smaller than predicted by about a factor of 2-3 for thin shields, but agrees well with predictions for thicker shields.

  12. A simple model of space radiation damage in GaAs solar cells

    NASA Technical Reports Server (NTRS)

    Wilson, J. W.; Stith, J. J.; Stock, L. V.

    1983-01-01

    A simple model is derived for the radiation damage of shallow junction gallium arsenide (GaAs) solar cells. Reasonable agreement is found between the model and specific experimental studies of radiation effects with electron and proton beams. In particular, the extreme sensitivity of the cell to protons stopping near the cell junction is predicted by the model. The equivalent fluence concept is of questionable validity for monoenergetic proton beams. Angular factors are quite important in establishing the cell sensitivity to incident particle types and energies. A fluence of isotropic incidence 1 MeV electrons (assuming infinite backing) is equivalent to four times the fluence of normal incidence 1 MeV electrons. Spectral factors common to the space radiations are considered, and cover glass thickness required to minimize the initial damage for a typical cell configuration is calculated. Rough equivalence between the geosynchronous environment and an equivalent 1 MeV electron fluence (normal incidence) is established.

  13. An Automated Method to Quantify Radiation Damage in Human Blood Cells

    SciTech Connect

    Gordon K. Livingston, Mark S. Jenkins and Akio A. Awa

    2006-07-10

    Cytogenetic analysis of blood lymphocytes is a well established method to assess the absorbed dose in persons exposed to ionizing radiation. Because mature lymphocytes circulate throughout the body, the dose to these cells is believed to represent the average whole body exposure. Cytogenetic methods measure the incidence of structural aberrations in chromosomes as a means to quantify DNA damage which occurs when ionizing radiation interacts with human tissue. Methods to quantify DNA damage at the chromosomal level vary in complexity and tend to be laborious and time consuming. In a mass casualty scenario involving radiological/nuclear materials, the ability to rapidly triage individuals according to radiation dose is critically important. For high-throughput screening for dicentric chromosomes, many of the data collection steps can be optimized with motorized microscopes coupled to automated slide scanning platforms.

  14. Atomic simulations of Fe/Ni multilayer nanocomposites on the radiation damage resistance

    NASA Astrophysics Data System (ADS)

    Chen, Feida; Tang, Xiaobin; Yang, Yahui; Huang, Hai; Liu, Jian; Li, Huan; Chen, Da

    2016-01-01

    We investigated the radiation damage resistance of the Fe/Ni multilayer nanocomposites by molecular dynamics. In the paper, two types of interface configuration with different orientation relationship were constructed. Their morphology evolution and number of final surviving defects induced by cascade collisions were discussed respectively. The interfaces of the two types of Fe/Ni multilayers kept distinct during the long-time relaxation before cascade. The comparison of surviving defects number produced by PKA with 5 keV at 100 K showed that the Fe/Ni multilayers have greater radiation tolerance than that of the bulk materials. However, the orientation relationship of the interface influences the defects self-healing capability greatly when the multilayers are irradiated by higher energy PKA or at high temperature. The radiation damage resistance of the Nishiyama - Wassermann type Fe/Ni multilayers which have larger lattice misfit is more stable than that of the Kurdjumov - Sachs type.

  15. The effect of space radiation on the induction of chromosome damage

    NASA Technical Reports Server (NTRS)

    George, K.; Wu, H.; Willingham, V.; Cucinotta, F. A.

    2001-01-01

    To obtain information on the cytogenetic damage caused by space radiation, chromosome exchanges in lymphocytes from crewmembers of long-term Mir missions, and a shorter duration shuttle mission, were examined using fluorescence in situ hybridization. A significant increase in chromosomal aberrations was observed after the long duration flights. The ratio of aberrations identified as complex was higher post-flight for some crewmembers, which is thought to be an indication of exposure to high-LET radiation. Ground-based studies have shown that the frequency of aberrations measured post-flight could be influenced by a mitotic delay in cells damaged by high-LET radiation and this effect could lower biological dose estimates. To counteract this effect, prematurely condensed chromosome (PCC) spreads were collected. Frequencies of aberrations in PCC were compared with those in metaphase spreads.

  16. Radiation induced apoptosis and initial DNA damage are inversely related in locally advanced breast cancer patients

    PubMed Central

    2010-01-01

    Background DNA-damage assays, quantifying the initial number of DNA double-strand breaks induced by radiation, have been proposed as a predictive test for radiation-induced toxicity. Determination of radiation-induced apoptosis in peripheral blood lymphocytes by flow cytometry analysis has also been proposed as an approach for predicting normal tissue responses following radiotherapy. The aim of the present study was to explore the association between initial DNA damage, estimated by the number of double-strand breaks induced by a given radiation dose, and the radio-induced apoptosis rates observed. Methods Peripheral blood lymphocytes were taken from 26 consecutive patients with locally advanced breast carcinoma. Radiosensitivity of lymphocytes was quantified as the initial number of DNA double-strand breaks induced per Gy and per DNA unit (200 Mbp). Radio-induced apoptosis at 1, 2 and 8 Gy was measured by flow cytometry using annexin V/propidium iodide. Results Radiation-induced apoptosis increased in order to radiation dose and data fitted to a semi logarithmic mathematical model. A positive correlation was found among radio-induced apoptosis values at different radiation doses: 1, 2 and 8 Gy (p < 0.0001 in all cases). Mean DSB/Gy/DNA unit obtained was 1.70 ± 0.83 (range 0.63-4.08; median, 1.46). A statistically significant inverse correlation was found between initial damage to DNA and radio-induced apoptosis at 1 Gy (p = 0.034). A trend toward 2 Gy (p = 0.057) and 8 Gy (p = 0.067) was observed after 24 hours of incubation. Conclusions An inverse association was observed for the first time between these variables, both considered as predictive factors to radiation toxicity. PMID:20868468

  17. Reduction of arsenite-enhanced ultraviolet radiation-induced DNA damage by supplemental zinc

    SciTech Connect

    Cooper, Karen L.; King, Brenee S.; Sandoval, Monica M.; Liu, Ke Jian; Hudson, Laurie G.

    2013-06-01

    Arsenic is a recognized human carcinogen and there is evidence that arsenic augments the carcinogenicity of DNA damaging agents such as ultraviolet radiation (UVR) thereby acting as a co-carcinogen. Inhibition of DNA repair is one proposed mechanism to account for the co-carcinogenic actions of arsenic. We and others find that arsenite interferes with the function of certain zinc finger DNA repair proteins. Furthermore, we reported that zinc reverses the effects of arsenite in cultured cells and a DNA repair target protein, poly (ADP-ribose) polymerase-1. In order to determine whether zinc ameliorates the effects of arsenite on UVR-induced DNA damage in human keratinocytes and in an in vivo model, normal human epidermal keratinocytes and SKH-1 hairless mice were exposed to arsenite, zinc or both before solar-simulated (ss) UVR exposure. Poly (ADP-ribose) polymerase activity, DNA damage and mutation frequencies at the Hprt locus were measured in each treatment group in normal human keratinocytes. DNA damage was assessed in vivo by immunohistochemical staining of skin sections isolated from SKH-1 hairless mice. Cell-based findings demonstrate that ssUVR-induced DNA damage and mutagenesis are enhanced by arsenite, and supplemental zinc partially reverses the arsenite effect. In vivo studies confirm that zinc supplementation decreases arsenite-enhanced DNA damage in response to ssUVR exposure. From these data we can conclude that zinc offsets the impact of arsenic on ssUVR-stimulated DNA damage in cells and in vivo suggesting that zinc supplementation may provide a strategy to improve DNA repair capacity in arsenic exposed human populations. - Highlights: • Low levels of arsenite enhance UV-induced DNA damage in human keratinocytes. • UV-initiated HPRT mutation frequency is enhanced by arsenite. • Zinc supplementation offsets DNA damage and mutation frequency enhanced by arsenite. • Zinc-dependent reduction of arsenite enhanced DNA damage is confirmed in vivo.

  18. Triple ion-beam studies of radiation damage effects in a 316LN austenitic alloy for a high power spallation neutron source

    SciTech Connect

    Lee, E.H.; Rao, G.R.; Hunn, J.D.; Rice, P.M.; Lewis, M.B.; Cook, S.W.; Farrell, K.; Mansur, L.K.

    1997-09-01

    Austenitic 316LN alloy was ion-irradiated using the unique Triple Ion Beam Facility (TIF) at ORNL to investigate radiation damage effects relevant to spallation neutron sources. The TIF was used to simulate significant features of GeV proton irradiation effects in spallation neutron source target materials by producing displacement damage while simultaneously injecting helium and hydrogen at appropriately high gas/dpa ratios. Irradiations were carried out at 80, 200, and 350 C using 3.5 MeV Fe{sup ++}, 360 keV He{sup +}, and 180 keV H{sup +} to accumulate 50 dpa by Fe, 10,000 appm of He, and 50,000 appm of H. Irradiations were also carried out at 200 C in single and dual ion beam modes. The specific ion energies were chosen to maximize the damage and the gas accumulation at a depth of {approximately} 1 {micro}m. Variations in microstructure and hardness of irradiated specimens were studied using transmission electron microscopy (TEM) and a nanoindentation technique, respectively. TEM investigation yielded varying damage defect microstructures, comprising black dots, faulted and unfaulted loops, and a high number density of fine bubbles (typically less than 1 nm in diameter). With increasing temperature, faulted loops had a tendency to unfault, and bubble microstructure changed from a bimodal size distribution to a unimodal distribution. Triple ion irradiations at the three temperatures resulted in similar increases in hardness of approximately a factor of two. Individually, Fe and He ions resulted in a similar magnitude of hardness increase, whereas H ions showed only a very small effect. The present study has yielded microstructural information relevant to spallation neutron source conditions and indicates that the most important concern may be radiation induced hardening and associated ductility loss.

  19. Resistance of (Fe,Ni)/sub 3/V long-range-ordered alloys to radiation damage

    SciTech Connect

    Braski, D.N.

    1980-01-01

    The (Fe,Ni)/sub 3/V long-range-ordered (LRO) alloys (path D) are being developed at ORNL for possible application as a first-wall material for a fusion reactor. Alloys with different Fe/Ni ratios have been screened for their resistance to radiation by irradiating them with 4 MeV Ni ions to 70 dpa at temperatures from 525 to 680/sup 0/C. Helium (8 at. ppM/dpa) and deuterium ions (28 at. ppM/dpa) were simultaneously injected to better simulate fusion reactor conditions. Alloy LRO-16 (31 wt % Ni) contained sigma phase and showed swelling behavior similar to the 20%-cold-worked 316 stainless steel that was used as an internal standard. LRO-20 (39.5 wt % Ni), without sigma phase, swelled slightly less than the 316 stainless steel. Both alloys demonstrated noticeably lower swelling behavior when their composition was changed to include 0.4 wt % ti.

  20. Helium-3 and Helium-4 acceleration by high power laser pulses for hadron therapy

    SciTech Connect

    Bulanov, S. S.; Esarey, E.; Schroeder, C. B.; Leemans, W. P.; Bulanov, S. V.; Margarone, D.; Korn, G.; Haberer, T.

    2015-06-24

    The laser driven acceleration of ions is considered a promising candidate for an ion source for hadron therapy of oncological diseases. Though proton and carbon ion sources are conventionally used for therapy, other light ions can also be utilized. Whereas carbon ions require 400 MeV per nucleon to reach the same penetration depth as 250 MeV protons, helium ions require only 250 MeV per nucleon, which is the lowest energy per nucleon among the light ions. This fact along with the larger biological damage to cancer cells achieved by helium ions, than that by protons, makes this species an interesting candidate for the laser driven ion source. Two mechanisms (Magnetic Vortex Acceleration and hole-boring Radiation Pressure Acceleration) of PW-class laser driven ion acceleration from liquid and gaseous helium targets are studied with the goal of producing 250 MeV per nucleon helium ion beams that meet the hadron therapy requirements. We show that He3 ions, having almost the same penetration depth as He4 with the same energy per nucleon, require less laser power to be accelerated to the required energy for the hadron therapy.

  1. Nanocrystal Ghosting: Extensive radiation damage in MgO induced by low-energy electrons

    NASA Astrophysics Data System (ADS)

    Sawyer, William; Frankenfield, Zachery; Kane, Kenneth

    Radiation damage in magnesium oxide has been an ongoing source of investigation. Early work was motivated by its simple cubic structure and its excellent electrical insulating properties over a wide range of temperatures and mechanical conditions. The goal was to determine its suitability as an electrical insulator in radiation intense environments including nuclear reactors and proposed nuclear fusion devices. During this period experimental results for irradiation of MgO using electrons with energies less than 500 keV produced very limited damage. These results, supported by theoretical arguments, lead to the conclusion that MgO was relatively impervious to damage from electrons with energies below this threshold. More recently its excellent insulating properties and relative mechanical stability combined with an increased interest in nanomaterials applications have created renewed interest in MgO. In this paper direct evidence is presented for extensive radiation damage in MgO nanocrystals from intense irradiation by electrons (2 x 10 4electrons/nm2 sec) with beam energies between 120 keV and 60 keV.

  2. The interaction of melanin with ionizing and UVC radiations: Characterization of thymine damage

    SciTech Connect

    Huselton, C.A.

    1988-01-01

    These studies were undertaken to determine whether melanin could protect DNA against the harmful effects of ionizing or UVC radiations. A simple, in vitro, model system was developed to evaluate eumelanin (Sigma melanin) as a radioprotector of solutions of 0.1 mM thymine or thymidine exposed to 570Gy of ionizing radiation. Sigma melanin was compared to several amino acids, other biomolecules or to other forms of melanin. To investigate the role of melanin as a passive screen of UVC radiation, melanotic (I{sub 3}), amelanotic (AMEL) cells (both derived from a Cloudman S91 melanoma) and non-melanotic (EMT6) cells were labelled with radioactive dTHd and exposed to 0, 1, 5 or 10KJ/m{sup 2} of UVC. The DNA was extracted; the bases hydrolyzed with concentrated HCl. Thymine bases were separated by reverse phase HPLC. No difference in dimer content was observed between I{sub 3} and AMEL cells, but EMT6 cells had nearly twice the amount of dimer. Overall thymine degradation was more pronounced in I{sub 3} cells than in the other two cell lines, due to the production of non-dimer thymine damage. This damage was identified as thymine glycol by HPLC and mass spectrometry. Melanin, upon exposure to UVC, appears to enhance thymine damage by producing oxidative damage.

  3. DNA Damage and Repair in Plants under Ultraviolet and Ionizing Radiations

    PubMed Central

    Gill, Sarvajeet S.; Gill, Ritu; Jha, Manoranjan; Tuteja, Narendra

    2015-01-01

    Being sessile, plants are continuously exposed to DNA-damaging agents present in the environment such as ultraviolet (UV) and ionizing radiations (IR). Sunlight acts as an energy source for photosynthetic plants; hence, avoidance of UV radiations (namely, UV-A, 315–400 nm; UV-B, 280–315 nm; and UV-C, <280 nm) is unpreventable. DNA in particular strongly absorbs UV-B; therefore, it is the most important target for UV-B induced damage. On the other hand, IR causes water radiolysis, which generates highly reactive hydroxyl radicals (OH•) and causes radiogenic damage to important cellular components. However, to maintain genomic integrity under UV/IR exposure, plants make use of several DNA repair mechanisms. In the light of recent breakthrough, the current minireview (a) introduces UV/IR and overviews UV/IR-mediated DNA damage products and (b) critically discusses the biochemistry and genetics of major pathways responsible for the repair of UV/IR-accrued DNA damage. The outcome of the discussion may be helpful in devising future research in the current context. PMID:25729769

  4. High and Low LET Radiation Differentially Induce Normal Tissue Damage Signals

    SciTech Connect

    Niemantsverdriet, Maarten; Goethem, Marc-Jan van; Bron, Reinier; Hogewerf, Wytse; Brandenburg, Sytze; Langendijk, Johannes A.; Luijk, Peter van; Coppes, Robert P.

    2012-07-15

    Purpose: Radiotherapy using high linear energy transfer (LET) radiation is aimed at efficiently killing tumor cells while minimizing dose (biological effective) to normal tissues to prevent toxicity. It is well established that high LET radiation results in lower cell survival per absorbed dose than low LET radiation. However, whether various mechanisms involved in the development of normal tissue damage may be regulated differentially is not known. Therefore the aim of this study was to investigate whether two actions related to normal tissue toxicity, p53-induced apoptosis and expression of the profibrotic gene PAI-1 (plasminogen activator inhibitor 1), are differentially induced by high and low LET radiation. Methods and Materials: Cells were irradiated with high LET carbon ions or low LET photons. Cell survival assays were performed, profibrotic PAI-1 expression was monitored by quantitative polymerase chain reaction, and apoptosis was assayed by annexin V staining. Activation of p53 by phosphorylation at serine 315 and serine 37 was monitored by Western blotting. Transfections of plasmids expressing p53 mutated at serines 315 and 37 were used to test the requirement of these residues for apoptosis and expression of PAI-1. Results: As expected, cell survival was lower and induction of apoptosis was higher in high -LET irradiated cells. Interestingly, induction of the profibrotic PAI-1 gene was similar with high and low LET radiation. In agreement with this finding, phosphorylation of p53 at serine 315 involved in PAI-1 expression was similar with high and low LET radiation, whereas phosphorylation of p53 at serine 37, involved in apoptosis induction, was much higher after high LET irradiation. Conclusions: Our results indicate that diverse mechanisms involved in the development of normal tissue damage may be differentially affected by high and low LET radiation. This may have consequences for the development and manifestation of normal tissue damage.

  5. Rapamycin‐induced autophagy sensitizes A549 cells to radiation associated with DNA damage repair inhibition

    PubMed Central

    Li, Yong; Liu, Fen; Wang, Yong; Li, Donghai; Guo, Fei; Xu, Liyao; Zeng, Zhengguo; Zhong, Xiaojun

    2016-01-01

    Abstract Background Autophagy has been reported to increase in cancer cells after radiation. However, it remains unknown whether increased autophagy as a result of radiation affects DNA damage repair and sensitizes cancer cells. In this study, the radiosensitization effect of rapamycin, a mammalian target of rapamycin inhibitor that induces autophagy, on human lung adenocarcinoma A549 cells was investigated. Methods A549 cells were treated with different concentrations of rapamycin. Cell viability was evaluated by methyl‐thiazolyl‐tetrazolium assay. Survival fraction values of A549 cells after radiotherapy were detected by colony formation assay. Autophagosome was observed by a transmission electron microscope. Furthermore, Western blot was employed to examine alterations in autophagy protein LC3 and p62, DNA damage protein γ–H2AX, and DNA damage repair proteins Rad51, Ku70, and Ku80. Rad51, Ku70, and Ku80 messenger ribonucleic acid (mRNA) expression levels were examined by real‐time polymerase chain reaction. Results Rapamycin suppressed A549 cell proliferation in dose and time‐dependent manners. An inhibitory concentration (IC) 10 dose of rapamycin could induce autophagy in A549 cells. Rapamycin combined with radiation significantly decreased the colony forming ability of cells, compared with rapamycin or radiation alone. Rapamycin and radiation combined increased γ–H2AX expression levels and decreased Rad51 and Ku80 expression levels, compared with single regimens. However, rapamycin treatment did not induce any change in Rad51, Ku70, and Ku80 mRNA levels, regardless of radiation. Conclusions These findings indicate that increasing autophagy sensitizes lung cancer cells to radiation. PMID:27385978

  6. Radiation damage in ferritic/martensitic steels for fusion reactors: a simulation point of view

    NASA Astrophysics Data System (ADS)

    Schäublin, R.; Baluc, N.

    2007-12-01

    Low activation ferritic/martensitic steels are good candidates for the future fusion reactors, for, relative to austenitic steels, their lower damage accumulation and moderate swelling under irradiation by the 14 MeV neutrons produced by the fusion reaction. Irradiation of these steels, e.g. EUROFER97, is known to produce hardening, loss of ductility, shift in ductile to brittle transition temperature and a reduction of fracture toughness and creep resistance starting at the lowest doses. Helium, produced by transmutation by the 14 MeV neutrons, is known to impact mechanical properties, but its effect at the microstructure level is still unclear. The mechanisms underlying the degradation of mechanical properties are not well understood, despite numerous studies on the evolution of the microstructure under irradiation. This impedes our ability to predict materials' behaviour at higher doses for use in the future fusion reactors. Simulations of these effects are now essential. An overview is presented on molecular dynamics simulations of the primary state of damage in iron and of the mobility of a dislocation, vector of plasticity, in the presence of a defect.

  7. Amelioration of radiation-induced hematopoietic and gastrointestinal damage by Ex-RAD® in mice

    PubMed Central

    Ghosh, Sanchita P.; Kulkarni, Shilpa; Perkins, Michael W.; Hieber, Kevin; Pessu, Roli L.; Gambles, Kristen; Maniar, Manoj; Kao, Tzu-Cheg; Seed, Thomas M.; Kumar, K. Sree

    2012-01-01

    The aim of the present study was to assess recovery from hematopoietic and gastrointestinal damage by Ex-RAD®, also known as ON01210.Na (4-carboxystyryl-4-chlorobenzylsulfone, sodium salt), after total body radiation. In our previous study, we reported that Ex-RAD, a small-molecule radioprotectant, enhances survival of mice exposed to gamma radiation, and prevents radiation-induced apoptosis as measured by the inhibition of radiation-induced protein 53 (p53) expression in cultured cells. We have expanded this study to determine best effective dose, dose-reduction factor (DRF), hematological and gastrointestinal protection, and in vivo inhibition of p53 signaling. A total of 500 mg/kg of Ex-RAD administered at 24 h and 15 min before radiation resulted in a DRF of 1.16. Ex-RAD ameliorated radiation-induced hematopoietic damage as monitored by the accelerated recovery of peripheral blood cells, and protection of granulocyte macrophage colony-forming units (GM-CFU) in bone marrow. Western blot analysis on spleen indicated that Ex-RAD treatment inhibited p53 phosphorylation. Ex-RAD treatment reduces terminal deoxynucleotidyl transferase mediated dUTP nick end labeling assay (TUNEL)-positive cells in jejunum compared with vehicle-treated mice after radiation injury. Finally, Ex-RAD preserved intestinal crypt cells compared with the vehicle control at 13 and 14 Gy. The results demonstrated that Ex-RAD ameliorates radiation-induced peripheral blood cell depletion, promotes bone marrow recovery, reduces p53 signaling in spleen and protects intestine from radiation injury. PMID:22843617

  8. From the Cover: Specific chemical and structural damage to proteins produced by synchrotron radiation

    NASA Astrophysics Data System (ADS)

    Weik, Martin; Ravelli, Raimond B. G.; Kryger, Gitay; McSweeney, Sean; Raves, Maria L.; Harel, Michal; Gros, Piet; Silman, Israel; Kroon, Jan; Sussman, Joel L.

    2000-01-01

    Radiation damage is an inherent problem in x-ray crystallography. It usually is presumed to be nonspecific and manifested as a gradual decay in the overall quality of data obtained for a given crystal as data collection proceeds. Based on third-generation synchrotron x-ray data, collected at cryogenic temperatures, we show for the enzymes Torpedo californica acetylcholinesterase and hen egg white lysozyme that synchrotron radiation also can cause highly specific damage. Disulfide bridges break, and carboxyl groups of acidic residues lose their definition. Highly exposed carboxyls, and those in the active site of both enzymes, appear particularly susceptible. The catalytic triad residue, His-440, in acetylcholinesterase, also appears to be much more sensitive to radiation damage than other histidine residues. Our findings have direct practical implications for routine x-ray data collection at high-energy synchrotron sources. Furthermore, they provide a direct approach for studying the radiation chemistry of proteins and nucleic acids at a detailed, structural level and also may yield information concerning putative "weak links" in a given biological macromolecule, which may be of structural and functional significance.

  9. From electrons to stars : modelling and mitigation of radiation damage effects on astronomical CCDs

    NASA Astrophysics Data System (ADS)

    Prod'homme, Thibaut

    2011-11-01

    The work presented in this thesis is part of an on-going effort to understand and mitigate the effects of radiation damage in astronomical CCDs. My research was motivated by and took place in the challenging context of the European Space Agency's (ESA) astrometric mission, Gaia, for which radiation damage has been considered since its conception as one of the most important threats to its scientific performance. In this context my research focused primarily on the modelling of the effects of radiation-induced Charge Transfer Inefficiency (CTI) supported by the analysis of the experimental test data. I developed the most detailed model to date of CTI in CCDs that enables simulating the operation of irradiated devices (Chapter 2). Using this model I have been able to verify and enhance our current understanding of CTI as well as support the characterization of CCDs and the understanding of experimental results (Chapters 3 and 6). As part of this research I conducted the comprehensive re-assessment of the performance of Gaia taking into account radiation damage (Chapters 3 and 4). Finally I took part in the effort of countering CTI by elaborating, testing, and improving a forward modelling approach at the image processing level to mitigate the CTI effects on the Gaia easurements (Chapters 3 and 5), as well as test and explore the potential of a specific hardware mitigation tool (Chapter 6).

  10. Early and Late Damages in Chromosome 3 of Human Lymphocytes After Radiation Exposure

    NASA Technical Reports Server (NTRS)

    Sunagawa, Mayumi; Mangala, Lingegowda; Zhang, Ye; Kahdim, Munira; Wilson, Bobby; Cucinotta, Francis A.; Wu, Honglu

    2011-01-01

    Tumor formation in humans or animals is a multi-step process. An early stage of cancer development is believed to be genomic instability (GI) which accelerates the mutation rate in the descendants of the cells surviving radiation exposure. GI is defined as elevated or persistent genetic damages occurring many generations after the cells are exposed. While early studies have demonstrated radiation-induced GI in several cell types as detected in endpoints such as mutation, apoptosis and damages in chromosomes, the dependence of GI on the quality of radiation remains uncertain. To investigate GI in human lymphocytes induced by both low- and high-LET radiation, we initially exposed white blood cells collected from healthy subjects to gamma rays in vitro, and cultured the cells for multiple generations. Chromosome aberrations were analyzed in cells collected at first mitosis post irradiation and at several intervals during the culture period. Among a number of biological endpoints planned for the project, the multi-color banding fluorescent in situ hybridization (mBAND) allows identification of inversions that were expected to be stable. We present here early and late chromosome aberrations detected with mBAND in chromosome 3 after gamma exposure. Comparison of chromosome damages in between human lymphocytes and human epithelial cells is also discussed

  11. New Modeling Approaches to Study DNA Damage by the Direct and Indirect Effects of Ionizing Radiation

    NASA Technical Reports Server (NTRS)

    Plante, Ianik; Cucinotta, Francis A.

    2012-01-01

    DNA is damaged both by the direct and indirect effects of radiation. In the direct effect, the DNA itself is ionized, whereas the indirect effect involves the radiolysis of the water molecules surrounding the DNA and the subsequent reaction of the DNA with radical products. While this problem has been studied for many years, many unknowns still exist. To study this problem, we have developed the computer code RITRACKS [1], which simulates the radiation track structure for heavy ions and electrons, calculating all energy deposition events and the coordinates of all species produced by the water radiolysis. In this work, we plan to simulate DNA damage by using the crystal structure of a nucleosome and calculations performed by RITRACKS. The energy deposition events are used to calculate the dose deposited in nanovolumes [2] and therefore can be used to simulate the direct effect of the radiation. Using the positions of the radiolytic species with a radiation chemistry code [3] it will be possible to simulate DNA damage by indirect effect. The simulation results can be compared with results from previous calculations such as the frequencies of simple and complex strand breaks [4] and with newer experimental data using surrogate markers of DNA double ]strand breaks such as . ]H2AX foci [5].

  12. [Use of vilosen in the treatment of radiation damage of the immune system].

    PubMed

    Tron'ko, M D; Sydorenko, D S; Bykova, L M; Goidash, M M; Boiko, M G; Synel'nikova, G L

    2001-01-01

    The possibility of vilosen usage for the immune system damage liquidation was studied. Rats obtained discrete rentgen irradiation during 1 month in the total dose of 4 Gr. Mice obtained internal 131I irradiation in a dose of 9.25 kBk/g. It was established that thymus and spleen masses, quantity of their cells, blood leukocytes and antibody production decreased by as external and internal irradiation. Irradiated animals treated with vilosen restored their immune system functional state partly or completely. The preparation was assumed to be used for the correction of immune system radiation damage. PMID:11296565

  13. Atomic and Molecular Data Needs for Radiation Damage Modeling: Multiscale Approach

    NASA Astrophysics Data System (ADS)

    Yakubovich, Alexander V.; Surdutovich, Eugene; Solov'yov, Andrey V.

    2011-05-01

    We present a brief overview of the multiscale approach towards understanding of the processes responsible for the radiation damage caused by energetic ions. This knowledge is very important, because it can be utilized in the ion-beam cancer therapy, which is one of the most advanced modern techniques to cure certain type of cancer. The central element of the multiscale approach is the theoretical evaluation and quantification of the DNA damage within cell environment. To achieve this goal one needs a significant amount of data on various atomic and molecular processes involved into the cascade of events starting with the ion entering and propagation in the biological medium and resulting in the DNA damage. The discussion of the follow up biological processes are beyond the scope of this brief overview. We consider different paths of the DNA damage and focus on the the illustration of the thermo-mechanical effects caused by the propagation of ions through the biological environment and in particular on the possibility of the creation of the shock waves in the vicinity of the ion tracks. We demonstrate that at the initial stages after ion's passage the shock wave is so strong that it can contribute to the DNA damage due to large pressure gradients developed at the distances of a few nanometers from the ionic tracks. This novel mechanism of the DNA damage provides an important contribution to the cumulative biodamage caused by low-energy secondary electrons, holes and free radicals.

  14. Atomic and Molecular Data Needs for Radiation Damage Modeling: Multiscale Approach

    SciTech Connect

    Yakubovich, Alexander V.; Solov'yov, Andrey V.; Surdutovich, Eugene

    2011-05-11

    We present a brief overview of the multiscale approach towards understanding of the processes responsible for the radiation damage caused by energetic ions. This knowledge is very important, because it can be utilized in the ion-beam cancer therapy, which is one of the most advanced modern techniques to cure certain type of cancer. The central element of the multiscale approach is the theoretical evaluation and quantification of the DNA damage within cell environment. To achieve this goal one needs a significant amount of data on various atomic and molecular processes involved into the cascade of events starting with the ion entering and propagation in the biological medium and resulting in the DNA damage. The discussion of the follow up biological processes are beyond the scope of this brief overview. We consider different paths of the DNA damage and focus on the the illustration of the thermo-mechanical effects caused by the propagation of ions through the biological environment and in particular on the possibility of the creation of the shock waves in the vicinity of the ion tracks. We demonstrate that at the initial stages after ion's passage the shock wave is so strong that it can contribute to the DNA damage due to large pressure gradients developed at the distances of a few nanometers from the ionic tracks. This novel mechanism of the DNA damage provides an important contribution to the cumulative biodamage caused by low-energy secondary electrons, holes and free radicals.

  15. Ideal sinks are not always ideal. Radiation damage accumulation in nanocomposites

    DOE PAGESBeta

    Uberuaga, Blas Pedro; Choudhury, Samrat; Caro, Alfredo

    2014-11-27

    Designing radiation tolerant materials is one of the primary challenges associated with advanced nuclear energy systems. One attractive route that has received much attention world-wide is to introduce a high density of sinks, often in the form of interfaces or secondary phases. Here, we develop a simple model of such nanocomposites and examine the ramifications of various factors on the overall radiation stability of the material. In particular, we determine how the distribution of secondary phases, the relative sink strength of those phases, and the irradiation temperature influence the radiation tolerance of the matrix. We find that the best scenariomore » is one in which the sinks have intermediate strength, transiently trapping defects before releasing them back into the matrix.This provides new insight into the optimal properties of nanocomposites for radiation damage environments.« less

  16. Mechanism for radiation damage resistance in yttrium oxide dispersion strengthened steels

    NASA Astrophysics Data System (ADS)

    Brodrick, J.; Hepburn, D. J.; Ackland, G. J.

    2014-02-01

    ODS steels based on yttrium oxide have been suggested as potential fusion reactor wall materials due to their observed radiation resistance properties. Presumably this radiation resistance can be related to the interaction of the particle with vacancies, self-interstitial atoms (SIAs) and other radiation damage debris. Density functional theory has been used to investigate this at the atomic scale. Four distinct interfaces, some based on HRTEM observations, between iron and yttrium oxide were investigated. It is been shown that the Y2O3-Fe interface acts as a strong trap with long-range attraction for both interstitial and vacancy defects, allowing recombination without altering the interface structure. The catalytic elimination of defects without change to the microstructure explains the improved behaviour of ODS steels with respect to radiation creep and swelling.

  17. Ideal sinks are not always ideal. Radiation damage accumulation in nanocomposites

    SciTech Connect

    Uberuaga, Blas Pedro; Choudhury, Samrat; Caro, Alfredo

    2014-11-27

    Designing radiation tolerant materials is one of the primary challenges associated with advanced nuclear energy systems. One attractive route that has received much attention world-wide is to introduce a high density of sinks, often in the form of interfaces or secondary phases. Here, we develop a simple model of such nanocomposites and examine the ramifications of various factors on the overall radiation stability of the material. In particular, we determine how the distribution of secondary phases, the relative sink strength of those phases, and the irradiation temperature influence the radiation tolerance of the matrix. We find that the best scenario is one in which the sinks have intermediate strength, transiently trapping defects before releasing them back into the matrix.This provides new insight into the optimal properties of nanocomposites for radiation damage environments.

  18. Radiation damage of PbWO 4 crystals due to irradiation by 60Co gamma rays

    NASA Astrophysics Data System (ADS)

    Kozma, Peter; Bajgar, Robert; Kozma, Petr

    2002-09-01

    Radiation resistivity of large tungstate crystals PbWO 4 from three suppliers has been studied for doses 10 4 Gy (10 6 rad) and 10 5 Gy (10 7 rad). Radiation resistivity was examined by the measurement of optical transmission through tungstate crystals before and after 60Co gamma-ray irradiations. The absolute degradation of transmission for 10 4 and 10 5 Gy doses at 480 nm wavelength of the peak emission of PbWO 4 doped with La 2+, was found to be lower than 12.3% and 14.2%, respectively. The results have been also compared with radiation hardness measurements for a large volume CeF 3 scintillation crystal. Complete recovery of radiation damage was observed between 10 and 15 days after irradiations.

  19. Radioactive transitions in the helium isoelectronic sequence

    NASA Technical Reports Server (NTRS)

    Dalgarno, A.

    1971-01-01

    The principles of the atomic spectrum theory are used to quantitatively analyze radiation transitions in two-electron helium-like atomic systems. Quantum theoretical methods, describing absorption and emission of a single photon in a radiative transition between two stationary states of an atomic system, reproduced the energy level diagram for the low lying states of helium. Reliable values are obtained from accurate variationally determined two-electron nonrelativistic wave functions for radiative transition probabilities of 2 3p states in the helium isoelectric sequence, and for the 2 1s and 2 3s1 states of the helium sequence.

  20. Nuclear radiation interference and damage effects in charged particle experiments for extended space missions.

    NASA Technical Reports Server (NTRS)

    Trainor, J. H.; Teegarden, B. J.

    1971-01-01

    Demonstration that meaningful galactic and solar cosmic radiation measurements can be carried out on deep space missions. The radioisotopic thermoelectric generators (RTGs) which must be used as a source of power and perhaps of heat are a problem, but with proper separation from the experiments, with orientation, and with some shielding the damage effects can be reduced to an acceptable level. The Pioneer spacecraft are crucial in that they are targeted at the heart of Jupiter's radiation belts, and should supply the details of those belts. The subsequent Grand Tour opportunities can be selected for those periods which result in larger distances of closest approach to Jupiter if necessary.

  1. How Magnetotactic Bacteria Respond to Radiation Induced Stress and Damage: Comparative Genomics Evidences for Evolutionary Adaptation

    NASA Astrophysics Data System (ADS)

    Wang, Y.; Pan, Y.

    2015-12-01

    Solar radiation and galactic cosmic radiation is believed to be major restriction factors influencing survival and evolution of life. On planet earth, geomagnetic field along with atmosphere protect living beings from the harmful radiation. During a geomagnetic reversal or excursion, however, the efflux of charged particles on earth surface would increase as the shielding effect of magnetic field decrease. The stratospheric ozone can also be partially stripped away by solar wind when the strength of the field is weak, leading to an increasing ultraviolet radiation penetration to the earth surface. However, studies on the mechanism of radiation induced stress and damage are focused only on bacteria that have no response to magnetic field. This study was motivated by the need to fill the gap upon knowledge of that on magnetic field sensitive microorganism. Magnetotactic bacteria (MTB) are a group of microbes that are able to synthesis intracellular nano-sized magnetic particles (named magnetosomes). These chain-arranged magnetosomes help MTB sense and swim along the magnetic field to find their optimal living environment efficiently. In this paper, in silico prediction of stress and damage repair genes in response to different radiation were carried out on the complete genome of four nonmagnetotactic and four magnetotactic spirilla. In silico analyses of the genomes of magnetic field sensitive and non-sensitive spirilla revealed: 1) all strains contain genes for regulate responses superoxide and peroxide stress, DNA pyrimidine dimer and string breaks; 2) non-magnetotactic spirilla have more genes dealing with oxidative stress, while magnetotactic spirilla may benefit from magnetotaxis by swimming into oxic-anoxic zone away from oxidative stress and direct radiation damage; yet, the lipid hydroperoxide peroxidase gene in MTB may be responsible for possible ROS generated by the membrane enveloped magnetite magnetosome; 3) magnetotactic spirilla possess SOS rec

  2. Study of the effect of dose-rate on radiation-induced damage to human erythrocytes

    NASA Astrophysics Data System (ADS)

    Krokosz, Anita; Koziczak, Renata; Gonciarz, Marta; Szweda-Lewandowska, Zofia

    2006-01-01

    Human erythrocytes suspended in an isotonic Na-phosphate buffer, pH 7.4 (hematocrit of 2%) were irradiated with γ-rays at three dose-rates of 66.7, 36.7, 25 Gy min -1 in order to investigate the influence of the dose-rate on radiation-induced membrane damage, hemoglobin oxidation and loss of reduced glutathione. The obtained results showed that such processes as erythrocyte hemolysis, lipid and protein destruction depend on the radiation dose-rate. The parameter values describing these processes showed an inverse dose-rate effect.

  3. Radiation damage in undoped CsI and CsI(Tl)

    SciTech Connect

    Woody, C.L.; Kierstead, J.A.; Levy, P.W.; Stoll, S.

    1992-12-01

    Radiation damage has been studied in undoped CsI and CsI(TI) crystals using {sup 60}Co gamma radiation for doses up to {approximately} 4.2 {times} 10{sup 6}. Samples from various manufacturers were measured ranging in size from 2.54 cm long cylinders to a 30 cm long block. Measurements were made on the change in optical transmission and scintillation light output as a function of dose. Although some samples showed a small change in transmission, a significant change in light output was observed for all samples. Recovery from damage was also studied as a function of time and exposure to UV light. A short lived phosphorescence was observed in undoped CsI, similar to the phosphorescence seen in CsI(TI).

  4. Radiation damage in undoped CsI and CsI(Tl)

    SciTech Connect

    Woody, C.L.; Kierstead, J.A.; Levy, P.W.; Stoll, S.

    1992-01-01

    Radiation damage has been studied in undoped CsI and CsI(TI) crystals using [sup 60]Co gamma radiation for doses up to [approximately] 4.2 [times] 10[sup 6]. Samples from various manufacturers were measured ranging in size from 2.54 cm long cylinders to a 30 cm long block. Measurements were made on the change in optical transmission and scintillation light output as a function of dose. Although some samples showed a small change in transmission, a significant change in light output was observed for all samples. Recovery from damage was also studied as a function of time and exposure to UV light. A short lived phosphorescence was observed in undoped CsI, similar to the phosphorescence seen in CsI(TI).

  5. Exploring long-time response to radiation damage in MgO

    NASA Astrophysics Data System (ADS)

    Uberuaga, B. P.; Smith, R.; Cleave, A. R.; Henkelman, G.; Grimes, R. W.; Voter, A. F.; Sickafus, K. E.

    2005-01-01

    Using a variety of computational modeling and simulation methods, we examine the production and long-time evolution of damage created in irradiated MgO. We find that the damage produced in low energy (order 1 keV) collision cascades typically consists of point defects and small defect clusters. Over long times, interstitials annihilate with vacancies and aggregate with other interstitials, forming larger clusters that exhibit surprising behavior. For example, a six-atom interstitial cluster is found to have extremely high mobility. The implications of highly-mobile large clusters are explored via a rate theory model and comparison to other materials. We conclude that successful modeling of radiation damage evolution in MgO requires explicit treatment of large interstitial clusters.

  6. Low doses of ionizing radiation to mammalian cells may rather control than cause DNA damage

    SciTech Connect

    Feinendegen, L.E.; Bond, V.P.; Sondhaus, C.A.; Altman, K.I.

    1998-12-31

    This report examines the origin of tissue effects that may follow from different cellular responses to low-dose irradiation, using published data. Two principal categories of cellular responses are considered. One response category relates to the probability of radiation-induced DNA damage. The other category consists of low-dose induced metabolic changes that induce mechanisms of DNA damage mitigation, which do not operate at high levels of exposure. Modeled in this way, tissue is treated as a complex adaptive system. The interaction of the various cellular responses results in a net tissue dose-effect relation that is likely to deviate from linearity in the low-dose region. This suggests that the LNT hypothesis should be reexamined. This paper aims at demonstrating tissue effects as an expression of cellular responses, both damaging and defensive, in relation to the energy deposited in cell mass, by use of microdosimetric concepts.

  7. Topical vitamin C protects porcine skin from ultraviolet radiation-induced damage.

    PubMed

    Darr, D; Combs, S; Dunston, S; Manning, T; Pinnell, S

    1992-09-01

    Ultraviolet radiation damage to the skin is due, in part, to the generation of reactive oxygen species. Vitamin C (L-ascorbic acid) functions as a biological co-factor and antioxidant due to its reducing properties. Topical application of vitamin C has been shown to elevate significantly cutaneous levels of this vitamin in pigs, and this correlates with protection of the skin from UVB damage as measured by erythema and sunburn cell formation. This protection is biological and due to the reducing properties of the molecule. Further, we provide evidence that the vitamin C levels of the skin can be severely depleted after UV irradiation, which would lower this organ's innate protective mechanism as well as leaving it at risk of impaired healing after photoinduced damage. In addition, vitamin C protects porcine skin from UVA-mediated phototoxic reactions (PUVA) and therefore shows promise as a broad-spectrum photoprotectant. PMID:1390169

  8. Measurement of DNA damage after exposure to 2450 MHz electromagnetic radiation.

    PubMed

    Malyapa, R S; Ahern, E W; Straube, W L; Moros, E G; Pickard, W F; Roti Roti, J L

    1997-12-01

    Recent reports suggest that exposure to 2450 MHz electromagnetic radiation causes DNA single-strand breaks (SSBs) and double-strand breaks (DSBs) in cells of rat brain irradiated in vivo (Lai and Singh, Bioelectromagnetics 16, 207-210, 1995; Int. J. Radiat. Biol. 69, 513-521, 1996). Therefore, we endeavored to determine if exposure of cultured mammalian cells in vitro to 2450 MHz radiation causes DNA damage. The alkaline comet assay (single-cell gel electrophoresis), which is reportedly the most sensitive method to assay DNA damage in individual cells, was used to measure DNA damage after in vitro 2450 MHz irradiation. Exponentially growing U87MG and C3H 10T1/2 cells were exposed to 2450 MHz continuous-wave (CW) radiation in specially designed radial transmission lines (RTLs) that provided relatively uniform microwave exposure. Specific absorption rates (SARs) were calculated to be 0.7 and 1.9 W/kg. Temperatures in the RTLs were measured in real time and were maintained at 37 +/- 0.3 degrees C. Every experiment included sham exposure(s) in an RTL. Cells were irradiated for 2 h, 2 h followed by a 4-h incubation at 37 degrees C in an incubator, 4 h and 24 h. After these treatments samples were subjected to the alkaline comet assay as described by Olive et al. (Exp. Cell Res. 198, 259-267, 1992). Images of comets were digitized and analyzed using a PC-based image analysis system, and the "normalized comet moment" and "comet length" were determined. No significant differences were observed between the test group and the controls after exposure to 2450 MHz CW irradiation. Thus 2450 MHz irradiation does not appear to cause DNA damage in cultured mammalian cells under these exposure conditions as measured by this assay. PMID:9399707

  9. Neutron flux spectra and radiation damage parameters for the Russian Bor-60 and SM-2 reactors

    SciTech Connect

    Karasiov, A.V.; Greenwood, L.R.

    1995-04-01

    The objective is to compare neutron irradiation conditions in Russian reactors and similar US facilities. Neutron fluence and spectral information and calculated radiation damage parameters are presented for the BOR-60 (Fast Experimental Reactor - 60 MW) and SM-2 reactors in Russia. Their neutron exposure characteristics are comparable with those of the Experimental Breeder Reactor (ERB-II), the Fast Flux Test Facility (FFTF), and the High Flux Isotope Reactor (HFIR) in the United States.

  10. Space Photovoltaic Research and Technology 1983. High Efficiency, Radiation Damage, and Blanket Technology

    NASA Technical Reports Server (NTRS)

    1984-01-01

    This three day conference, sixth in a series that began in 1974, was held at the NASA Lewis Research Center on October 18-20, 1983. The conference provided a forum for the discussion of space photovoltaic systems, their research status, and program goals. Papers were presented and workshops were held in a variety of technology areas, including basic cell research, advanced blanket technology, and radiation damage.

  11. Performance characteristics and radiation damage results from the Fermilab E706 silicon microstrip detector system

    NASA Astrophysics Data System (ADS)

    Engels, E.; Mani, S.; Orris, D.; Shepard, P. F.; Weerasundara, P. D.; Choudhary, B. C.; Joshi, U.; Kapoor, V.; Shivpuri, R.; Baker, W.; Berg, D.; Carey, D.; Johnstone, C.; Nelson, C.; Bromberg, C.; Brown, D.; Huston, J.; Miller, R.; Nguyen, A.; Benson, R.; Lukens, P.; Ruddick, K.; Alverson, G.; Faissler, W.; Garelick, D.; Glaubman, M.; Kourbanis, I.; Lirakis, C.; Pothier, E.; Sinanidis, A.; Wu, G.-H.; Yasuda, T.; Yosef, C.; Easo, S.; Hartman, K.; Oh, B. Y.; Toothacker, W.; Whitmore, J.; Ballocchi, G.; Debarbaro, L.; Desoi, W.; Fanourakis, G.; Ferbel, T.; Ginther, G.; Gutierrez, P.; Lanaro, A.; Lobkowicz, F.; Mansour, J.; Pedeville, G.; Prebys, E.; Skow, D.; Slattery, P.; Varelas, N.; Zielinski, M.

    1989-07-01

    A charged particle spectrometer containing a 7120-channel silicon microstrip detector system, one component of Fermilab experiment E706 to study direct photon production in hadron-hadron collisions, was utilized in a run in which 6 million events were recorded. We describe the silicon system, provide early results of track and vertex reconstruction, and present data on the radiation damage to the silicon wafers resulting from the narrow high intensity beam.

  12. QUANTIFICATION OF ACTINIDE ALPHA-RADIATION DAMAGE IN MINERALS AND CERAMICS

    SciTech Connect

    Farnan, Ian E.; Cho, Herman M.; Weber, William J.

    2007-01-11

    There are large amounts of heavy alpha-emitters in nuclear waste and nuclear materials inventories stored in various sites around the world. These include plutonium and minor actinides such as americium and curium. In preparation for geological disposal there is a consensus that actinides that have been separated from spent nuclear fuel should be immobilised within mineral-based ceramics rather than glass. Over the long-term, the alpha-decay taking place in these ceramics will severely disrupt their crystalline structure and reduce their durability. A fundamental property in predicting cumulative radiation damage is the number of atoms permanently displaced per alpha–decay. Currently, this number is estimated as 1000-2000 atoms/alpha decay event. Here, we report nuclear magnetic resonance, spin-counting experiments that measure close to 5000 atoms/alpha decay event in radiation damaged natural zircons. New radiological NMR measurements on highly radioactive, 239Pu zircon show damage similar to that created by 238U and 232Th in mineral zircons at the same dose, indicating no significant effect of dose rate. Based on these measurements, the initially crystalline structure of a 10 wt% 239Pu zircon would be amorphous after only 1400 years in a geological repository. These measurements establish a basis for assessing the long-term structural durability of actinide-containing ceramics based on an atomistic understanding of the fundamental damage event.

  13. Recombinant Human Epidermal Growth Factor Accelerates Recovery of Mouse Small Intestinal Mucosa After Radiation Damage

    SciTech Connect

    Lee, Kang Kyoo; Jo, Hyang Jeong; Hong, Joon Pio; Lee, Sang-wook Sohn, Jung Sook; Moon, Soo Young; Yang, Sei Hoon; Shim, Hyeok; Lee, Sang Ho; Ryu, Seung-Hee; Moon, Sun Rock

    2008-07-15

    Purpose: To determine whether systemically administered recombinant human epidermal growth factor (rhEGF) accelerates the recovery of mouse small intestinal mucosa after irradiation. Methods and Materials: A mouse mucosal damage model was established by administering radiation to male BALB/c mice with a single dose of 15 Gy applied to the abdomen. After irradiation, rhEGF was administered subcutaneously at various doses (0.04, 0.2, 1.0, and 5.0 mg/kg/day) eight times at 2- to 3-day intervals. The evaluation methods included histologic changes of small intestinal mucosa, change in body weight, frequency of diarrhea, and survival rate. Results: The recovery of small intestinal mucosa after irradiation was significantly improved in the mice treated with a high dose of rhEGF. In the mice that underwent irradiation without rhEGF treatment, intestinal mucosal ulceration, mucosal layer damage, and severe inflammation occurred. The regeneration of villi was noticeable in mice treated with more than 0.2 mg/kg rhEGF, and the villi recovered fully in mice given more than 1 mg/kg rhEGF. The frequency of diarrhea persisting for more than 3 days was significantly greater in the radiation control group than in the rhEGF-treated groups. Conclusions: Systemic administration of rhEGF accelerates recovery from mucosal damage induced by irradiation. We suggest that rhEGF treatment shows promise for the reduction of small intestinal damage after irradiation.

  14. Summary of the radiation damage studies of the SDC dopants in polystyrene

    SciTech Connect

    Pla-Dalmau, A.; Foster, G.W.; Zhang, G.

    1993-12-22

    Approximately 80 commercially available fluorescent organic compounds were studied as dopants in a polystyrene matrix for possible use in wavelength shifting (WLS) fibers. The goal was to find a new green- emitting WLS fiber which would outperform in light yield and decay time the currently available fiber doped with K-27. Therefore the fluorescent compounds of interest should exhibit the following spectroscopic characteristics in polystyrene: {lambda}{sub abs} = 400--450 nm,{lambda}{sub em} = 450--550 nm, {tau} = 3--7 ns and quantum efficiency of minimum 0.7. Polystyrene samples doped with different fluorescent compounds were prepared and characterized. Of all the compounds tested, only a series of coumarins exhibited the spectroscopic characteristics of interest. Radiation damage studies had to be performed on these samples in order to condusively determine if they were better candidates than K-27 for green WLS fibers. AU samples except those showing opacity or deep coloration were irradiated. They were, however, separated in two sets. Radiation damage set No. 20 was mainly formed by the coumarin derivatives. Radiation damage set No. 22 was based on the remaining samples. The irradiations were performed at the Phoenix Memorial Laboratory using a {sup 60}Co source. Both sets were exposed to a total dose of 10 Mrad in air, at a dose rate of 1.8 Mrad/h. Transmittance measurements were recorded before and after irradiation, and after annealing. After irradiation, the samples were annealed in oxygen to accelerate the recovery process.

  15. Radiation-Induced Cytogenetic Damage as a Predictor of Cancer Risk for Protons and Fe Ions

    NASA Technical Reports Server (NTRS)

    Williams, Jerry R.

    1999-01-01

    We have successfully completed the series of experiments planned for year 1 and the first part of year 2 measuring the induction of chromosome aberrations induced in multiple cell types by three model space radiations: Fe-ions, protons and photons. Most of these data have now been compiled and a significant part subjected to detailed data analyses, although continuing data analysis is an important part of our current and future efforts. These analyses are directed toward defining the patterns of chromosomal damage induction by the three radiations and the extent to which such patterns are dependent on the type of cell irradiated. Our studies show significant differences, both quantitatively and qualitatively, between response of different cell types to these radiations however there is an overall pattern that characterizes each type of radiation in most cell lines. Thus our data identifies general dose-response patterns for each radiation for induction of multiple types of chromosomal aberrations but also identifies significant differences in response between some cell types. Specifically, we observe significant resistance for induction of aberrations in rat mammary epithelial cells when they are irradiated in vivo and assayed in vitro. Further, we have observed some remarkable differences in susceptibility to certain radiation-induced aberrations in cells whose genome has been modulated for two cancer- relevant genes, TP53 and CDKNIA. This data, if confirmed, may represent the first evidence of gene-specific differences in cellular metabolism of damage induced by densely-ionizing radiation that confers substantial sensitivity to protons compared to photons.

  16. A Role for Lsm1p in Response to Ultraviolet-Radiation Damage in Saccharomyces cerevisiae

    PubMed Central

    Spicakova, Tatiana; McCann, Kelly; Brown, J. Martin

    2008-01-01

    A genome-wide screen in Saccharomyces cerevisiae identified LSM1 as a new gene affecting sensitivity to ultraviolet (UV) radiation. Lsm1p is a member of a cytoplasmic complex composed of Lsm1p–7p that interacts with the yeast mRNA degradation machinery. To investigate the potential role of Lsm1p in response to UV radiation, we constructed double mutant strains in which LSM1 was deleted in combination with a representative gene from each of three known yeast DNA repair pathways. Our results show that lsm1Δ increases the UV-radiation sensitivity of the rad1Δ and rad51Δ mutants, but not the rad18Δ mutant, placing LSM1 within the post-replication repair/damage tolerance pathway (PRR). When combined with other deletions affecting PRR, lsm1Δ increases the UV-radiation sensitivity of the rev3Δ, rad30Δ and pol30-K164R mutants but not rad5Δ. Furthermore, the UV-radiation sensitivity phenotype of lsm1Δ is partially rescued by mutations in genes involved in 3′ to 5′ mRNA degradation, and mutations predicted to function in RNA interactions confer the most UV-radiation sensitivity. Together, these results suggest that Lsm1p may confer protection against UV-radiation damage by protecting the 3′ ends of mRNAs from exosome-dependent 3′ to 5′ degradation as part of a novel RAD5-mediated, PCNA-K164 ubiquitylation-independent subpathway of PRR. PMID:19024647

  17. Radiation damage and luminescence properties of gamma aluminum oxynitride transparent ceramic

    NASA Astrophysics Data System (ADS)

    Du, Xinhua; Yao, Shiyue; Jin, Xihai; Chen, Haohong; Li, Weifeng; Liang, Bo

    2015-09-01

    This paper reports on the radiation damage of gamma aluminum oxynitride (γ-AlON) transparent ceramic, which remarkably degrades UV-vis transparency and hence limits its applications in optoelectronic devices. The radiation-induced optical absorption of the as-sintered γ-AlON consists of at least two subbands: one is in the UV region with a peak at 270 nm and the other optical absorption band centers at 550 nm, covering the whole visible light spectrum, which makes the sample colored. Interestingly, all the radiation-induced color centers can be completely ‘bleached’ by low temperature annealing. In the thermoluminescence curve, we observed a broad luminescence in the range of 25-300 °C with the peak at 120 °C. Furthermore, the x-ray excited luminescence spectra revealed that there exist multiple emission centers in the γ-AlON. Based on this experimental fact, the radiation damage and luminescent mechanisms were studied. These optical properties of the γ-AlON are considered to be related to defect states. In the as-sintered γ-AlON, charge balancing is realized by the co-existence of \\text{V}\\text{Al}\\prime\\prime \\prime and \\text{O}\\text{N}\\bullet , and the predominant defect form is ≤ft[\\text{V}\\text{Al}\\prime\\prime \\prime-\\text{3O}\\text{N}\\bullet\\right] , which is optically inactive and no optical absorption occurs. However, isolated \\text{V}\\text{Al}\\prime\\prime \\prime and \\text{O}\\text{N}\\bullet can be formed by irradiation and it is these that are responsible for the radiation damage of γ-AlON transparent ceramic. In the end, the UV absorption and visible-light absorption in the irradiated sample were ascribed to VAl-related and ON-related intrinsic defects, respectively.

  18. Hopping diffusion of helium isotopes from samples of lunar soil

    NASA Astrophysics Data System (ADS)

    Anufriev, G. S.

    2010-10-01

    This paper reports on a detailed study of diffusion of helium isotopes from a sample of lunar soil (weight, 3.3 mg; bulk grain size, <74 μm; sampling depth, 118 cm in a 1.6-m-long core of lunar soil brought from the Moon by the Soviet automatic station Luna-24). The studies have been performed using step heating in the temperature range 300-1000°C in combination with a mass spectrometric isotope analysis of helium extracted at each temperature step. It has been demonstrated that the diffusion does not obey Fick’s law, which should be attributed to a large number of radiation damages in crystals of lunar soil minerals and can be described in terms of the formalism accepted for jump diffusion. The diffusion activation energy for both helium isotopes (4He, 3He) has been found to be identical and equal to 0.5 eV, and the frequency factors amount to 0.51 and 0.59 s-1, respectively. The random errors σ in the determination of these parameters are approximately equal to 5%. The lunar soil delivered to the Earth loses helium during the storage. At the beginning of the storage at room temperature, one gram of the lunar material under investigation loses approximately 3 × 109 helium atoms every second. It has been revealed that the jump diffusion of helium exhibits a strong isotopic effect: the light isotope 3He escapes at substantially higher rates. In order to prevent helium losses accompanied by isotope fractionation, the brought lunar soil should be stored at a low temperature.

  19. Spallation radiation damage and dosimetry for accelerator transmutation of waste applications

    SciTech Connect

    Wechsler, M.S.; Lin, C.; Ferguson, P.D.; Sommer, W.F.

    1993-10-01

    Proposals are currently being made for systems to treat radioactive waste based on the use of accelerator-driven neutron sources. A linear proton accelerator with energies as high as 1600 MeV and currents up to 250 ma are anticipated for the driver. The neutron fluxes may reach up to 10{sup 20} neutrons/m{sup 2}s as generated by the spallation reactions that occur when the protons strike target materials. Calculations are described to determine radiation fluxes and flux spectra inherent in such systems and to estimate likely radiation effects on system components. The calculations use LAHET, a Monte Carlo high-energy transport code, and MCNP, a generalized-geometry, coupled neutron-photon Monte Carlo transport code. Cross sections for displacement and helium production are presented for spallation neutrons of energies from 21 MeV to 1600 MeV for Inconel 718 (Ni plus 18.5, 18.5, 5.1, and 3 wt % of Cr, Fe, Nb, and Mo, respectively), an alloy that is used for the proton beam entry window in several accelerators. In addition, results for this alloy are presented for the primary knocked-on atom (PKA) spectrum and the transmutation yield for 1600 MeV incident neutrons.

  20. Dissecting the molecular mechanism of ionizing radiation-induced tissue damage in the feather follicle.

    PubMed

    Chen, Xi; Liao, Chunyan; Chu, Qiqi; Zhou, Guixuan; Lin, Xiang; Li, Xiaobo; Lu, Haijie; Xu, Benhua; Yue, Zhicao

    2014-01-01

    Ionizing radiation (IR) is a common therapeutic agent in cancer therapy. It damages normal tissue and causes side effects including dermatitis and mucositis. Here we use the feather follicle as a model to investigate the mechanism of IR-induced tissue damage, because any perturbation of feather growth will be clearly recorded in its regular yet complex morphology. We find that IR induces defects in feather formation in a dose-dependent manner. No abnormality was observed at 5 Gy. A transient, reversible perturbation of feather growth was induced at 10 Gy, leading to defects in the feather structure. This perturbation became irreversible at 20 Gy. Molecular and cellular analysis revealed P53 activation, DNA damage and repair, cell cycle arrest and apoptosis in the pathobiology. IR also induces patterning defects in feather formation, with disrupted branching morphogenesis. This perturbation is mediated by cytokine production and Stat1 activation, as manipulation of cytokine levels or ectopic Stat1 over-expression also led to irregular feather branching. Furthermore, AG-490, a chemical inhibitor of Stat1 signaling, can partially rescue IR-induced tissue damage. Our results suggest that the feather follicle could serve as a useful model to address the in vivo impact of the many mechanisms of IR-induced tissue damage. PMID:24586618

  1. Inactivation of NADPH Oxidases NOX4 and NOX5 Protects Human Primary Fibroblasts from Ionizing Radiation-Induced DNA Damage

    PubMed Central

    Weyemi, Urbain; Redon, Christophe E.; Aziz, Towqir; Choudhuri, Rohini; Maeda, Daisuke; Parekh, Palak R.; Bonner, Michael Y.; Arbiser, Jack L.; Bonner, William M.

    2015-01-01

    Human exposure to ionizing radiation from medical procedures has increased sharply in the last three decades. Recent epidemiological studies suggest a direct relationship between exposure to ionizing radiation and health problems, including cancer incidence. Therefore, minimizing the impact of radiation exposure in patients has become a priority in the development of future clinical practices. Crucial players in radiation-induced DNA damage include reactive oxygen species (ROS), but the sources of these have remained elusive. To the best of our knowledge, we show here for the first time that two members of the ROS-generating NADPH oxidase family (NOXs), NOX4 and NOX5, are involved in radiation-induced DNA damage. Depleting these two NOXs in human primary fibroblasts resulted in reduced levels of DNA damage as measured by levels of radiation-induced foci, a marker of DNA double-strand breaks (DSBs) and the comet assay coupled with increased cell survival. NOX involvement was substantiated with fulvene-5, a NOXs-specific inhibitor. Moreover, fulvene-5 mitigated radiation-induced DNA damage in human peripheral blood mononuclear cells ex vivo. Our results provide evidence that the inactivation of NOXs protects cells from radiation-induced DNA damage and cell death. These findings suggest that NOXs inhibition may be considered as a future pharmacological target to help minimize the negative effects of radiation exposure for millions of patients each year. PMID:25706776

  2. Reduction of arsenite-enhanced ultraviolet radiation-induced DNA damage by supplemental zinc.

    PubMed

    Cooper, Karen L; King, Brenee S; Sandoval, Monica M; Liu, Ke Jian; Hudson, Laurie G

    2013-06-01

    Arsenic is a recognized human carcinogen and there is evidence that arsenic augments the carcinogenicity of DNA damaging agents such as ultraviolet radiation (UVR) thereby acting as a co-carcinogen. Inhibition of DNA repair is one proposed mechanism to account for the co-carcinogenic actions of arsenic. We and others find that arsenite interferes with the function of certain zinc finger DNA repair proteins. Furthermore, we reported that zinc reverses the effects of arsenite in cultured cells and a DNA repair target protein, poly (ADP-ribose) polymerase-1. In order to determine whether zinc ameliorates the effects of arsenite on UVR-induced DNA damage in human keratinocytes and in an in vivo model, normal human epidermal keratinocytes and SKH-1 hairless mice were exposed to arsenite, zinc or both before solar-simulated (ss) UVR exposure. Poly (ADP-ribose) polymerase activity, DNA damage and mutation frequencies at the Hprt locus were measured in each treatment group in normal human keratinocytes. DNA damage was assessed in vivo by immunohistochemical staining of skin sections isolated from SKH-1 hairless mice. Cell-based findings demonstrate that ssUVR-induced DNA damage and mutagenesis are enhanced by arsenite, and supplemental zinc partially reverses the arsenite effect. In vivo studies confirm that zinc supplementation decreases arsenite-enhanced DNA damage in response to ssUVR exposure. From these data we can conclude that zinc offsets the impact of arsenic on ssUVR-stimulated DNA damage in cells and in vivo suggesting that zinc supplementation may provide a strategy to improve DNA repair capacity in arsenic exposed human populations. PMID:23523584

  3. Radiation damage characterization in reactor pressure vessel steels with nonlinear ultrasound

    SciTech Connect

    Matlack, K. H.; Kim, J.-Y.; Wall, J. J.; Qu, J.; Jacobs, L. J.

    2014-02-18

    Nuclear generation currently accounts for roughly 20% of the US baseload power generation. Yet, many US nuclear plants are entering their first period of life extension and older plants are currently undergoing assessment of technical basis to operate beyond 60 years. This means that critical components, such as the reactor pressure vessel (RPV), will be exposed to higher levels of radiation than they were originally intended to withstand. Radiation damage in reactor pressure vessel steels causes microstructural changes such as vacancy clusters, precipitates, dislocations, and interstitial loops that leave the material in an embrittled state. The development of a nondestructive evaluation technique to characterize the effect of radiation exposure on the properties of the RPV would allow estimation of the remaining integrity of the RPV with time. Recent research has shown that nonlinear ultrasound is sensitive to radiation damage. The physical effect monitored by nonlinear ultrasonic techniques is the generation of higher harmonic frequencies in an initially monochromatic ultrasonic wave, arising from the interaction of the ultrasonic wave with microstructural features such as dislocations, precipitates, and their combinations. Current findings relating the measured acoustic nonlinearity parameter to increasing levels of neutron fluence for different representative RPV materials are presented.

  4. Ionizing radiation-induced DNA injury and damage detection in patients with breast cancer

    PubMed Central

    Borrego-Soto, Gissela; Ortiz-López, Rocío; Rojas-Martínez, Augusto

    2015-01-01

    Abstract Breast cancer is the most common malignancy in women. Radiotherapy is frequently used in patients with breast cancer, but some patients may be more susceptible to ionizing radiation, and increased exposure to radiation sources may be associated to radiation adverse events. This susceptibility may be related to deficiencies in DNA repair mechanisms that are activated after cell-radiation, which causes DNA damage, particularly DNA double strand breaks. Some of these genetic susceptibilities in DNA-repair mechanisms are implicated in the etiology of hereditary breast/ovarian cancer (pathologic mutations in the BRCA 1 and 2 genes), but other less penetrant variants in genes involved in sporadic breast cancer have been described. These same genetic susceptibilities may be involved in negative radiotherapeutic outcomes. For these reasons, it is necessary to implement methods for detecting patients who are susceptible to radiotherapy-related adverse events. This review discusses mechanisms of DNA damage and repair, genes related to these functions, and the diagnosis methods designed and under research for detection of breast cancer patients with increased radiosensitivity. PMID:26692152

  5. Repair of clustered DNA damage caused by high LET radiation in human fibroblasts

    NASA Technical Reports Server (NTRS)

    Rydberg, B.; Lobrich, M.; Cooper, P. K.; Chatterjee, A. (Principal Investigator)

    1998-01-01

    It has recently been demonstrated experimentally that DNA damage induced by high LET radiation in mammalian cells is non-randomly distributed along the DNA molecule in the form of clusters of various sizes. The sizes of such clusters range from a few base-pairs to at least 200 kilobase-pairs. The high biological efficiency of high LET radiation for induction of relevant biological endpoints is probably a consequence of this clustering, although the exact mechanisms by which the clustering affects the biological outcome is not known. We discuss here results for induction and repair of base damage, single-strand breaks and double-strand breaks for low and high LET radiations. These results are discussed in the context of clustering. Of particular interest is to determine how clustering at different scales affects overall rejoining and fidelity of rejoining of DNA double-strand breaks. However, existing methods for measuring repair of DNA strand breaks are unable to resolve breaks that are close together in a cluster. This causes problems in interpretation of current results from high LET radiation and will require new methods to be developed.

  6. Protective Effects of Polysaccharides from Soybean Meal Against X-ray Radiation Induced Damage in Mouse Spleen Lymphocytes

    PubMed Central

    Yao, Lei; Wang, Zhenyu; Zhao, Haitian; Cheng, Cuilin; Fu, Xiaoyi; Liu, Jiaren; Yang, Xin

    2011-01-01

    The aim of this study was to investigate radioprotective effect of the polysaccharides from soybean meal (SMP) against X-ray radiation-induced damage in mouse spleen lymphocytes. MTT and comet assay were performed to evaluate SMP’s ability to prevent cell death and DNA damage induced by radiation. The results show that, X-ray radiation (30 KV, 10 mA, 8 min (4 Gy)) can significantly increase cell death and DNA fragmentation of mouse spleen lymphocytes. Pretreatment with SMP for 2 h before radiation could increase cell viability, moreover, the SMP can reduce X-ray radiation-induced DNA damage. The percentage of tail DNA and the tail moment of the SMP groups were significantly lower than those of the radiation alone group (p < 0.05). These results suggest SMP may be a good candidate as a radioprotective agent. PMID:22174652

  7. Oxidative Lung Damage Resulting from Repeated Exposure to Radiation and Hyperoxia Associated with Space Exploration

    PubMed Central

    Pietrofesa, Ralph A; Turowski, Jason B; Arguiri, Evguenia; Milovanova, Tatyana N; Solomides, Charalambos C; Thom, Stephen R; Christofidou-Solomidou, Melpo

    2013-01-01

    Background Spaceflight missions may require crewmembers to conduct Extravehicular Activities (EVA) for repair, maintenance or scientific purposes. Pre-breathe protocols in preparation for an EVA entail 100% hyperoxia exposure that may last for a few hours (5-8 hours), and may be repeated 2-3 times weekly. Each EVA is associated with additional challenges such as low levels of total body cosmic/galactic radiation exposure that may present a threat to crewmember health and therefore, pose a threat to the success of the mission. We have developed a murine model of combined, hyperoxia and radiation exposure (double-hit) in the context of evaluating countermeasures to oxidative lung damage associated with space flight. In the current study, our objective was to characterize the early and chronic effects of repeated single and double-hit challenge on lung tissue using a novel murine model of repeated exposure to low-level total body radiation and hyperoxia. This is the first study of its kind evaluating lung damage relevant to space exploration in a rodent model. Methods Mouse cohorts (n=5-15/group) were exposed to repeated: a) normoxia; b) >95% O2 (O2); c) 0.25Gy single fraction gamma radiation (IR); or d) a combination of O2 and IR (O2+IR) given 3 times per week for 4 weeks. Lungs were evaluated for oxidative damage, active TGFβ1 levels, cell apoptosis, inflammation, injury, and fibrosis at 1, 2, 4, 8, 12, 16, and 20 weeks post-initiation of exposure. Results Mouse cohorts exposed to all challenge conditions displayed decreased bodyweight compared to untreated controls at 4 and 8 weeks post-challenge initiation. Chronic oxidative lung damage to lipids (malondialdehyde levels), DNA (TUNEL, cleaved Caspase 3, cleaved PARP positivity) leading to apoptotic cell death and to proteins (nitrotyrosine levels) was elevated all treatment groups. Importantly, significant systemic oxidative stress was also noted at the late phase in mouse plasma, BAL fluid, and urine. Importantly

  8. Postmortem examination of 22 pancreatic carcinoma patients treated with helium ion irradiation

    SciTech Connect

    Woodruff, K.H.; Castro, J.R.; Quivey, J.M.; Saunders, W.M.; Chen, G.T.; Lyman, J.T.; Pitluck, S.; Tobias, C.A.; Walton, R.E.; Peters, T.C.

    1984-02-01

    Postmortem findings are available in this report in 22 patients with pancreatic carcinoma treated with helium ions at Lawrence Berkeley Laboratory; California. This represents the largest group evaluated histologically in the literature and is the first report evaluating effects of particle radiation in pancreatic tissue. Patient survival after therapy averaged 9 months. Most died of infection and/or pulmonary emboli. Local control was achieved in 27%. The pancreatic tumors had histologically more severe radiation changes than nontumor bearing pancreas. Irradiated bone marrow was severely hypocellular, and irradiated skin was atrophic. Five patients had radiation injury in the gastrointestinal tract. The spinal cord, liver, and kidneys showed no damage. This study demonstrates the safety of helium particle irradiation with present therapeutic planning. Injury to tumor was seen without excessive damage to adjacent tissues.

  9. Dose Rate Effects on Damage and Recovery of Radiation Hard Glass Under Gamma Irradiation

    NASA Astrophysics Data System (ADS)

    Menchini, Francesca; Baccaro, Stefania; Cemmi, Alessia; di Sarcina, Ilaria; Fiore, Salvatore; Piegari, Angela

    2014-06-01

    Optical systems employed in space missions are subjected to high fluxes of energetic particles. Their optical properties should be stable throughout the whole mission, to avoid a possible failure of the experiments. Radiation hard glasses are widely used as substrates or windows in high-energy applications, due to their resistance in hostile environments where energetic particles and γ rays are present. In this work we have irradiated radiation resistant glass windows by γ rays from a 60Co source at several doses, from 50 to 3×l05 Gy, and at two different dose rates. The optical properties of the samples have been monitored and the effects of radiations have been measured. Moreover, a partial recovery of the damage has been observed after the end of irradiation. The effects depend on the irradiation dose rate.

  10. An evaluation of radiation damage to solid state components flown in low earth orbit satellites.

    PubMed

    Shin, Myung-Won; Kim, Myung-Hyun

    2004-01-01

    The effects of total ionising radiation dose upon commercial off-the-shelf semiconductors fitted to satellites operating in low Earth orbit (LEO) conditions was evaluated. The evaluation was performed for the Korea Institute of Technology SATellite-1, (KITSAT-1) which was equipped with commercial solid state components. Two approximate calculation models for space radiation shielding were developed. Verification was performed by comparing the results with detailed three-dimensional calculations using the Monte-Carlo method and measured data from KITSAT-1. It was confirmed that the developed approximate models were reliable for satellite shielding calculations. It was also found that commercial semiconductor devices, which were not radiation hardened, could be damaged within their lifetime due to the total ionising dose they are subject to in the LEO environment. To conclude, an intensive shielding analysis should be considered when commercial devices are used. PMID:15103058

  11. Stability of Radiation Induced Chromosome Damage in Human Peripheral Blood Lymphocytes

    NASA Technical Reports Server (NTRS)

    Cucinotta, F. A.; George, K.; Willingham, V.

    2006-01-01

    Chromosome damage in an individual's peripheral blood lymphocytes can be an indicator of radiation exposure and this data can be used to evaluate dose after accidental or occupational exposure. Evidence suggests that the yield of chromosome damage in lymphocytes is also a relevant biomarker of cancer risk in humans that reflects individual cancer susceptibility. It follows that biomonitoring studies can be used to uncover subjects who are particularly susceptible to radiation damage and therefore at higher risk of cancer. Translocations and other stable aberrations are commonly believed to persist in peripheral blood cells for many years after exposure, and it has been suggested that translocations can be used for assessing retrospective radiation doses or chronic exposures. However, recent investigations suggest that translocations might not always persist indefinitely. We measured chromosome aberrations in the blood lymphocytes of six astronauts before their respective missions of approximately 3 to 6 months onboard the international space station, and again at various intervals up to 5 years after flight. In samples collected a few days after return to earth, the yield of chromosome translocations had significantly increased compared with preflight values, and results indicate that biodosimetry estimates lie within the range expected from physical dosimetry. However, for five of the astronauts, follow up analysis revealed a temporal decline in translocations with half-lives ranging from 10 to 58 months. The yield of exchanges remained unchanged for the sixth astronaut during an observation period of 5 months post-flight. These results may indicate complications with the use of stable aberrations for retrospective dose reconstruction and could affect cancer risk predictions that are estimated from yields of chromosome damage obtained shortly after exposure.

  12. Spatiotemporal characterization of ionizing radiation induced DNA damage foci and their relation to chromatin organization

    SciTech Connect

    Costes, Sylvain V; Chiolo, Irene; Pluth, Janice M.; Barcellos-Hoff, Mary Helen; Jakob, Burkhard

    2009-09-15

    DNA damage sensing proteins have been shown to localize to the sites of DSB within seconds to minutes following ionizing radiation (IR) exposure, resulting in the formation of microscopically visible nuclear domains referred to as radiation-induced foci (RIF). This review characterizes the spatio-temporal properties of RIF at physiological doses, minutes to hours following exposure to ionizing radiation, and it proposes a model describing RIF formation and resolution as a function of radiation quality and nuclear densities. Discussion is limited to RIF formed by three interrelated proteins ATM (Ataxia telangiectasia mutated), 53BP1 (p53 binding protein 1) and ?H2AX (phosphorylated variant histone H2AX). Early post-IR, we propose that RIF mark chromatin reorganization, leading to a local nuclear scaffold rigid enough to keep broken DNA from diffusing away, but open enough to allow the repair machinery. We review data indicating clear kinetic and physical differences between RIF emerging from dense and uncondensed regions of the nucleus. At later time post-IR, we propose that persistent RIF observed days following exposure to ionizing radiation are nuclear ?scars? marking permanent disruption of the chromatin architecture. When DNA damage is resolved, such chromatin modifications should not necessarily lead to growth arrest and it has been shown that persistent RIF can replicate during mitosis. Thus, heritable persistent RIF spanning over tens of Mbp may affect the transcriptome of a large progeny of cells. This opens the door for a non DNA mutation-based mechanism of radiation-induced phenotypes.

  13. Intermediate structures in radiation damaged titanite (CaTiSiO5): a Raman spectroscopic study.

    PubMed

    Zhang, Ming; Salje, Ekhard K H; Redfern, Simon A T; Bismayer, Ulrich; Groat, Lee A

    2013-03-20

    Effects of radiation damage and thermal annealing on the crystal structure of natural titanite (CaTiSiO(5)) were studied using Raman spectroscopy. The results show that well crystallized natural titanites generally have the P2(1)/a structure at the unit cell level, in contrast to the A2/a symmetry reported previously. Radiation caused by naturally incorporated impurities (such as U and Th) leads to structural damage and amorphization in titanite, as evidenced by a significant loss of band intensity, spectral broadening and frequency shifts. Additional bands (e.g. near 574 and 650 cm(-1)) occur in weakly or partially metamict titanite due to the formation of an intermediate phase (with the A2/a symmetry). Raman spectra of titanite thermal glasses showed features different from those of metamict titanite, especially in the Ti-O and Si-O stretching regions. The effect of thermal annealing is strongly affected by the initial degrees of damage that the sample experienced. Weakly damaged titanite samples showed that annealing leads to a structural recovery, and the spectral patterns of these recovered crystals are consistent with the P2(1)/a symmetry. Highly damaged titanite starts to recrystallize into an A2/a phase near 700-800 K, and additional structural modification occurs when annealed at 1300-1400 K, which involves significant change in broad Ti-O features. However, in terms of bandwidths, the metamict samples are far from fully recovered even on being annealed at 1300-1400 K. PMID:23407062

  14. Global radiation damage at 300 and 260 K with dose rates approaching 1 MGy s{sup −1}

    SciTech Connect

    Warkentin, Matthew; Badeau, Ryan; Hopkins, Jesse B.; Mulichak, Anne M.; Keefe, Lisa J.; Thorne, Robert E.

    2012-02-01

    Approximately half of global radiation damage to thaumatin crystals can be outrun at 260 K if data are collected in less than 1 s. Global radiation damage to 19 thaumatin crystals has been measured using dose rates from 3 to 680 kGy s{sup −1}. At room temperature damage per unit dose appears to be roughly independent of dose rate, suggesting that the timescales for important damage processes are less than ∼1 s. However, at T = 260 K approximately half of the global damage manifested at dose rates of ∼10 kGy s{sup −1} can be outrun by collecting data at 680 kGy s{sup −1}. Appreciable sample-to-sample variability in global radiation sensitivity at fixed dose rate is observed. This variability cannot be accounted for by errors in dose calculation, crystal slippage or the size of the data sets in the assay.

  15. a Study of Biophysical Mechanisms of Damage by Ionizing Radiation to Mammalian Cells in Vitro.

    NASA Astrophysics Data System (ADS)

    Chen, Chun-Zhang

    Available from UMI in association with The British Library. An extensive survey made of published survival data of damage by ionizing radiation to mammalian cells in vitro has led to the new conclusion that the damage is determined by the specific ionization or the mean free path between ionizing events along the charged particle tracks. The optimum damage is observed when the mean free path is equivalent to the DNA double strand spacing of 1.8 nm. Therefore, the biological mechanism of ionizing radiation to mammalian cells in vitro is intra track dominant. A 100 keV electron accelerator has been constructed and commissioned to produce a broad beam irradiation field of greater than 1 cm diameter. The fluence rate may be adjusted from 10^8cm^ {-2}sec^{-1} downwards to enable further development as a chronic irradiation facility. Another new feature of the accelerator is that it incorporates a differential vacuum system which permits irradiation of the monolayer cell cultures to be carried out in normal pressure. Experiments of irradiation to Chinese hamster cells, by ^{241}Am alpha particles at low fluence rate, have supplied satisfactory data for testing a new DNA-rupture model which is under development. For V79 cells irradiated at a low fluence rate of 10^5cm^{ -2}min^{-1}, when survival data were fitted into the model, new biophysical parameters were extracted and a proposal was made that the repair phenomenon of cellular survival at very low doses is determined by three time factors: the irradiation time, the damage fixation time and the repair time. The values obtained were 3-4 hours for the mean repair time, and more than 10 hours for the damage to be considered permanent. Details of the monolayer cell culture technique developed and used in the present experiments are described. Consideration has been given to the significance of the results obtained from the study in radiation protection and in radiotherapy. In future studies it is recommended that more

  16. Effect of high power CO2 and Yb:YAG laser radiation on the characteristics of TIG arc in atmospherical pressure argon and helium

    NASA Astrophysics Data System (ADS)

    Wu, Shikai; Xiao, Rongshi

    2015-04-01

    The effects of laser radiation on the characteristics of the DC tungsten inert gas (TIG) arc were investigated by applying a high power slab CO2 laser and a Yb:YAG disc laser. Experiment results reveal that the arc voltage-current curve shifts downwards, the arc column expands, and the arc temperature rises while the high power CO2 laser beam vertically interacts with the TIG arc in argon. With the increase of the laser power, the voltage-current curve of the arc shifts downwards more significantly, and the closer the laser beam impingement on the arc to the cathode, the more the decrease in arc voltage. Moreover, the arc column expansion and the arc temperature rise occur mainly in the region between the laser beam incident position and the anode. However, the arc characteristics hardly change in the cases of the CO2 laser-helium arc and YAG laser-arc interactions. The reason is that the inverse Bremsstrahlung absorption coefficients are greatly different due to the different electron densities of the argon and helium arcs and the different wave lengths of CO2 and YAG lasers.

  17. Study the radiation damage effects in Si microstrip detectors for future HEP experiments

    NASA Astrophysics Data System (ADS)

    Lalwani, Kavita; Jain, Geetika; Dalal, Ranjeet; Ranjan, Kirti; Bhardwaj, Ashutosh

    2016-07-01

    Silicon (Si) detectors are playing a key role in High Energy Physics (HEP) experiments due to their superior tracking capabilities. In future HEP experiments, like upgrade of the Compact Muon Solenoid (CMS) experiment at the Large Hadron Collider (LHC), CERN, the silicon tracking detectors will be operated in a very intense radiation environment. This leads to both surface and bulk damage in Si detectors, which in turn will affect the operating performance of Si detectors. It is important to complement the measurements of the irradiated Si strip detectors with device simulation, which helps in understanding of both the device behavior and optimizing the design parameters needed for the future Si tracking system. An important ingredient of the device simulation is to develop a radiation damage model incorporating both bulk and surface damage. In this work, a simplified two-trap model is incorporated in device simulation to describe the type-inversion. Further, an extensive simulation of effective doping density as well as electric field profile is carried out at different temperatures for various fluences.

  18. Imaging of radiation damage using complementary field ion microscopy and atom probe tomography.

    PubMed

    Dagan, Michal; Hanna, Luke R; Xu, Alan; Roberts, Steve G; Smith, George D W; Gault, Baptiste; Edmondson, Philip D; Bagot, Paul A J; Moody, Michael P

    2015-12-01

    Radiation damage in tungsten and a tungsten-tantalum alloy, both of relevance to nuclear fusion research, has been characterized using a combination of field ion microscopy (FIM) imaging and atom probe tomography (APT). While APT provides 3D analytical imaging with sub-nanometer resolution, FIM is capable of imaging the arrangements of single atoms on a crystal lattice and has the potential to provide insights into radiation induced crystal damage, all the way down to its smallest manifestation--a single vacancy. This paper demonstrates the strength of combining these characterization techniques. In ion implanted tungsten, it was found that atomic scale lattice damage is best imaged using FIM. In certain cases, APT reveals an identifiable imprint in the data via the segregation of solute and impurities and trajectory aberrations. In a W-5at%Ta alloy, a combined APT-FIM study was able to determine the atomic distribution of tantalum inside the tungsten matrix. An indirect method was implemented to identify tantalum atoms inside the tungsten matrix in FIM images. By tracing irregularities in the evaporation sequence of atoms imaged with FIM, this method enables the benefit of FIM's atomic resolution in chemical distinction between the two species. PMID:25794822

  19. Energy Thresholds of DNA Damage Induced by UV Radiation: An XPS Study.

    PubMed

    Gomes, P J; Ferraria, A M; Botelho do Rego, A M; Hoffmann, S V; Ribeiro, P A; Raposo, M

    2015-04-30

    This work stresses on damage at the molecular level caused by ultraviolet radiation (UV) in the range from 3.5 to 8 eV, deoxyribonucleic acid (DNA) films observed by X-ray photoelectron spectroscopy (XPS). Detailed quantitative XPS analysis, in which all the amounts are relative to sodium-assumed not to be released from the samples, of the carbon, oxygen, and particularly, nitrogen components, reveals that irradiation leads to sugar degradation with CO-based compounds release for energies above 6.9 eV and decrease of nitrogen groups which are not involved in hydrogen bonding at energies above 4.2 eV. Also the phosphate groups are seen to decrease to energies above 4.2 eV. Analysis of XPS spectra allowed to conclude that the damage on bases peripheral nitrogen atoms are following the damage on phosphates. It suggests that very low kinetic energy photoelectrons are ejected from the DNA bases, as a result of UV light induced breaking of the phosphate ester groups which forms a transient anion with resonance formation and whereby most of the nitrogen DNA peripheral groups are removed. The degree of ionization of DNA was observed to increase with radiation energy, indicating that the ionized phosphate groups are kept unchanged. This result was interpreted by the shielding of phosphate groups caused by water molecules hydration near sodium atoms. PMID:25844940

  20. Molecular dynamics study of the bulk temperature effect on primary radiation damage in uranium dioxide

    NASA Astrophysics Data System (ADS)

    Martin, G.; Sabathier, C.; Wiktor, J.; Maillard, S.

    2015-06-01

    The effect of bulk temperature on the primary damage induced by a displacement cascade was investigated in uranium dioxide using classical molecular dynamics simulations. In this study, the Morelon potentials were used to model the middle-range interactions between the atoms that constitute the host matrix during the radiation events. Cascades were initiated by accelerating a uranium primary knock-on atom at 10keV inside a perfect UO2 lattice at a temperature between 700K and 1800K , a range which comprises in-pile temperatures of oxide fuels in light water reactors in standard operating conditions. Cascade overlap sequences were also simulated at 700K and 1400K in order to study the radiation damage accumulation in the oxide fuel. This study reveals the maximum damage level which the material can accommodate for decreases with the temperature. Furthermore the direct formation of vacancy clusters under irradiation is considerably slowed down above 1000K , notably during cascade overlap sequences.

  1. Prevention of UVB Radiation-induced Epidermal Damage by Expression of Heat Shock Protein 70*

    PubMed Central

    Matsuda, Minoru; Hoshino, Tatsuya; Yamashita, Yasuhiro; Tanaka, Ken-ichiro; Maji, Daisuke; Sato, Keizo; Adachi, Hiroaki; Sobue, Gen; Ihn, Hironobu; Funasaka, Yoko; Mizushima, Tohru

    2010-01-01

    Irradiation with UV light, especially UVB, causes epidermal damage via the induction of apoptosis, inflammatory responses, and DNA damage. Various stressors, including UV light, induce heat shock proteins (HSPs) and the induction, particularly that of HSP70, provides cellular resistance to such stressors. The anti-inflammatory activity of HSP70, such as its inhibition of nuclear factor kappa B (NF-κB), was recently revealed. These in vitro results suggest that HSP70 protects against UVB-induced epidermal damage. Here we tested this idea by using transgenic mice expressing HSP70 and cultured keratinocytes. Irradiation of wild-type mice with UVB caused epidermal damage such as induction of apoptosis, which was suppressed in transgenic mice expressing HSP70. UVB-induced apoptosis in cultured keratinocytes was suppressed by overexpression of HSP70. Irradiation of wild-type mice with UVB decreased the cutaneous level of IκB-α (an inhibitor of NF-κB) and increased the infiltration of leukocytes and levels of pro-inflammatory cytokines and chemokines in the epidermis. These inflammatory responses were suppressed in transgenic mice expressing HSP70. In vitro, the overexpression of HSP70 suppressed the expression of pro-inflammatory cytokines and chemokines and increased the level of IκB-α in keratinocytes irradiated with UVB. UVB induced an increase in cutaneous levels of cyclobutane pyrimidine dimers and 8-hydroxy-2′-deoxyguanosine, both of which were suppressed in transgenic mice expressing HSP70. This study provides genetic evidence that HSP70 protects the epidermis from UVB-induced radiation damage. The findings here also suggest that the protective action of HSP70 is mediated by anti-apoptotic, anti-inflammatory, and anti-DNA damage effects. PMID:20018843

  2. Angular distributions and polarization fractions of helium resonance radiation (n 1P - 1 1S) in the extreme ultraviolet

    NASA Technical Reports Server (NTRS)

    Mumma, M. J.; Misakian, M.; Jackson, W. M.; Faris, J. L.

    1973-01-01

    Angular intensity distributions of helium (n 1P - 1 1S) resonance photons with respect to the exciting electron beam are presented. The angular intensity distributions were measured at selected electron impact energies from 25 eV (near threshold) to 150 eV. Polarization fractions (Pi) were obtained by analyzing the data in terms of the theoretical relation between angular intensity distribution and Pi, i.e. Iota (theta) = Iota (90) (1 - Pi sq cos theta). The experimental values for Pi are compared with recent theoretical results and with previous experimental values for the (3 1P - 2 1S) transition.

  3. Repair Welding of Irradiated Materials: Modeling of Helium Bubble Distributions for Determining Crack-Free Welding Procedures

    SciTech Connect

    Feng, Zhili; Wilkowski, Gery

    2002-07-01

    In this paper, a computational simulation study is presented on the prediction of helium bubble evolution during repair welding of irradiated 304 stainless steel. Realistic spatial and temporal temperature and stress evolution during welding were obtained from simulation of the repair welding operation using the finite element model approach. The helium bubble evolution model by Kawano et al. was adopted as a user subroutine in the finite element model to predict the spatial distribution and temporal evolution of the helium bubble size and density in the heat-affected zone (HAZ) of partial penetration welds. Comparisons with experimental results available in open literature show that the predicted average helium bubble sizes were consistent with those observed experimentally under similar conditions. In addition, the computer simulation revealed strong spatial variation of helium bubble size due to the differences in combined thermal and stress conditions experienced in different locations in the HAZ. The predicted location of the maximum helium bubble agreed well with the observed helium-induced cracking site. The effect of welding heat input and welding speed was also investigated numerically. The modeling approach adopted in this study could be used as a cost-effective tool to quantitatively correlate the welding condition, radiation damage, and the likelihood of cracking, under the influence of welding-induced thermal and stress cycles. The model will also be useful in studying the degradation of properties from helium bubble formation of post-welded structures, even if a successful weld is made. (authors)

  4. Persistence of Space Radiation Induced Cytogenetic Damage in the Blood Lymphocytes of Astronauts

    NASA Technical Reports Server (NTRS)

    George, Kerry; Cucinotta, Francis A.

    2008-01-01

    Cytogenetic damage in astronaut's peripheral blood lymphocytes is a useful in vivo marker of space radiation induced damage. Moreover, if radiation induced chromosome translocations persist in peripheral blood lymphocytes for many years, as has been assumed, they could potentially be used to measure retrospective doses or prolonged low dose rate exposures. However, as more data becomes available, evidence suggests that the yield of translocations may decline with time after exposure, at least in the case of space radiation exposures. We present our latest follow-up measurements of chromosome aberrations in astronauts blood lymphocytes assessed by FISH painting and collected a various times beginning directly after return from space to several years after flight. For most individuals the analysis of individual time-courses for translocations revealed a temporal decline of yields with different half-lives. Since the level of stable aberrations depends on the interplay between natural loss of circulating T-lymphocytes and replenishment from the stem or progenitor cells, the differences in the rates of decay could be explained by inter-individual variation in lymphocyte turn over. Biodosimetry estimates derived from cytogenetic analysis of samples collected a few days after return to earth lie within the range expected from physical dosimetry. However, a temporal decline in yields may indicate complications with the use of stable aberrations for retrospective dose reconstruction, and the differences in the decay time may reflect individual variability in risk from space radiation exposure. In addition, limited data on multiple flights show a lack of correlation between time in space and translocation yields. Data from one crewmember who has participated in two separate long-duration space missions and has been followed up for over 10 years provides limited information on the effect of repeat flights and show a possible adaptive response to space radiation exposure.

  5. Space Radiation Induced Cytogenetic Damage in the Blood Lymphocytes of Astronauts

    NASA Technical Reports Server (NTRS)

    George, K.; Cucinotta, F. A.

    2008-01-01

    Cytogenetic analysis of astronauts blood lymphocytes provides a direct in vivo measurement of space radiation damage, which takes into account individual radiosensitivity and considers the influence of microgravity and other stress conditions. We present our latest analyses of chromosome damage in astronauts blood lymphocytes assessed by fluorescence in situ hybridization (FISH) chromosome painting and collected at various times beginning directly after return from space to several years after flight. Dose was derived from frequencies of chromosome exchanges using preflight calibration curves, and the Relative Biological Effect (RBE) was estimated by comparison with individually measured physically absorbed doses. Values for average RBE were compared to the average quality factor (Q), from direct measurements of the lineal energy spectra using a tissue-equivalent proportional counter (TEPC) and radiation transport codes. Results prove that cytogenetic biodosimetry analyses on blood collected within a week or two of return from space provides a reliable estimate of equivalent radiation dose and risk after protracted exposure to space radiation of a few months or more. However, data collected several months or years after flight suggests that the yield of chromosome translocations may decline with time after the mission, indicating that retrospective doses may be more difficult to estimate. In addition, limited data on multiple flights show a lack of correlation between time in space and translocation yields. Data from one crewmember, who has participated in two separate long-duration space missions and has been followed up for over 10 years, provide limited information on the effect of repeat flights and show a possible adaptive response to space radiation exposure.

  6. Influence of complex impurity centres on radiation damage in wide-gap metal oxides

    NASA Astrophysics Data System (ADS)

    Lushchik, A.; Lushchik, Ch.; Popov, A. I.; Schwartz, K.; Shablonin, E.; Vasil'chenko, E.

    2016-05-01

    Different mechanisms of radiation damage of wide-gap metal oxides as well as a dual influence of impurity ions on the efficiency of radiation damage have been considered on the example of binary ionic MgO and complex ionic-covalent Lu3Al5O12 single crystals. Particular emphasis has been placed on irradiation with ∼2 GeV heavy ions (197Au, 209Bi, 238U, fluence of 1012 ions/cm2) providing extremely high density of electronic excitations within ion tracks. Besides knock-out mechanism for Frenkel pair formation, the additional mechanism through the collapse of mobile discrete breathers at certain lattice places (e.g., complex impurity centres) leads to the creation of complex defects that involve a large number of host atoms. The experimental manifestations of the radiation creation of intrinsic and impurity antisite defects (Lu|Al or Ce|Al - a heavy ion in a wrong cation site) have been detected in LuAG and LuAG:Ce3+ single crystals. Light doping of LuAG causes a small enhancement of radiation resistance, while pair impurity centres (for instance, Ce|Lu-Ce|Al or Cr3+-Cr3+ in MgO) are formed with a rise of impurity concentration. These complex impurity centres as well as radiation-induced intrinsic antisite defects (Lu|Al strongly interacting with Lu in a regular site) tentatively serve as the places for breathers collapse, thus decreasing the material resistance against dense irradiation.

  7. Direct Radiation Damage to Crystalline DNA: What is the Source of Unaltered Base Release?

    PubMed Central

    Razskazovskiy, Yuriy; Debije, Michael G.; Bernhard, William A.

    2008-01-01

    The radiation chemical yields of unaltered base release have been measured in three crystalline double-stranded DNA oligomers after X irradiation at 4 K. The yields of released bases are between 10 and 20% of the total free radical yields measured at 4 K. Using these numbers, we estimate that the yield of DNA strand breaks due to the direct effect is about 0.1 μmol J−1. The damage responsible for base release is independent of the base type (C, G, A or T) and is not scavenged by anthracycline drugs intercalated in the DNA. For these reasons, reactions initiated by the hydroxyl radical have been ruled out as the source of base release. Since the intercalated anthracycline scavenges electrons and holes completely but does not inhibit base release, the possibility for damage transfer from the bases to the sugars can also be ruled out. The results are consistent with a model in which primary radical cations formed directly on the sugar-phosphate backbone react by two competing pathways: deprotonation, which localizes the damage on the sugar, and hole tunneling, which transfers the damage to the base stack. Quantitative estimates indicate that these two processes are approximately equally efficient. PMID:10761004

  8. Radiation damage of the PCO Pixelfly VGA CCD camera of the BES system on KSTAR tokamak

    NASA Astrophysics Data System (ADS)

    Náfrádi, Gábor; Kovácsik, Ákos; Pór, Gábor; Lampert, Máté; Un Nam, Yong; Zoletnik, Sándor

    2015-01-01

    A PCO Pixelfly VGA CCD camera which is part a of the Beam Emission Spectroscopy (BES) diagnostic system of the Korea Superconducting Tokamak Advanced Research (KSTAR) used for spatial calibrations, suffered from serious radiation damage, white pixel defects have been generated in it. The main goal of this work was to identify the origin of the radiation damage and to give solutions to avoid it. Monte Carlo N-Particle eXtended (MCNPX) model was built using Monte Carlo Modeling Interface Program (MCAM) and calculations were carried out to predict the neutron and gamma-ray fields in the camera position. Besides the MCNPX calculations pure gamma-ray irradiations of the CCD camera were carried out in the Training Reactor of BME. Before, during and after the irradiations numerous frames were taken with the camera with 5 s long exposure times. The evaluation of these frames showed that with the applied high gamma-ray dose (1.7 Gy) and dose rate levels (up to 2 Gy/h) the number of the white pixels did not increase. We have found that the origin of the white pixel generation was the neutron-induced thermal hopping of the electrons which means that in the future only neutron shielding is necessary around the CCD camera. Another solution could be to replace the CCD camera with a more radiation tolerant one for example with a suitable CMOS camera or apply both solutions simultaneously.

  9. Trans-Differentiation of Neural Stem Cells: A Therapeutic Mechanism Against the Radiation Induced Brain Damage

    PubMed Central

    Kang, Bong Gu; Lee, Se Jeong; Kim, Kang Ho; Yang, Heekyoung; Lee, Young-Ae; Cho, Yu Jin; Im, Yong-Seok; Lee, Dong-Sup; Lim, Do-Hoon; Kim, Dong Hyun; Um, Hong-Duck; Lee, Sang-Hun; Lee, Jung-II; Nam, Do-Hyun

    2012-01-01

    Radiation therapy is an indispensable therapeutic modality for various brain diseases. Though endogenous neural stem cells (NSCs) would provide regenerative potential, many patients nevertheless suffer from radiation-induced brain damage. Accordingly, we tested beneficial effects of exogenous NSC supplementation using in vivo mouse models that received whole brain irradiation. Systemic supplementation of primarily cultured mouse fetal NSCs inhibited radiation-induced brain atrophy and thereby preserved brain functions such as short-term memory. Transplanted NSCs migrated to the irradiated brain and differentiated into neurons, astrocytes, or oligodendrocytes. In addition, neurotrophic factors such as NGF were significantly increased in the brain by NSCs, indicating that both paracrine and replacement effects could be the therapeutic mechanisms of NSCs. Interestingly, NSCs also differentiated into brain endothelial cells, which was accompanied by the restoration the cerebral blood flow that was reduced from the irradiation. Inhibition of the VEGF signaling reduced the migration and trans-differentiation of NSCs. Therefore, trans-differentiation of NSCs into brain endothelial cells by the VEGF signaling and the consequential restoration of the cerebral blood flow would also be one of the therapeutic mechanisms of NSCs. In summary, our data demonstrate that exogenous NSC supplementation could prevent radiation-induced functional loss of the brain. Therefore, successful combination of brain radiation therapy and NSC supplementation would provide a highly promising therapeutic option for patients with various brain diseases. PMID:22347993

  10. DNA base damage by reactive oxygen species, oxidizing agents, and UV radiation.

    PubMed

    Cadet, Jean; Wagner, J Richard

    2013-02-01

    Emphasis has been placed in this article dedicated to DNA damage on recent aspects of the formation and measurement of oxidatively generated damage in cellular DNA in order to provide a comprehensive and updated survey. This includes single pyrimidine and purine base lesions, intrastrand cross-links, purine 5',8-cyclonucleosides, DNA-protein adducts and interstrand cross-links formed by the reactions of either the nucleobases or the 2-deoxyribose moiety with the hydroxyl radical, one-electron oxidants, singlet oxygen, and hypochlorous acid. In addition, recent information concerning the mechanisms of formation, individual measurement, and repair-rate assessment of bipyrimidine photoproducts in isolated cells and human skin upon exposure to UVB radiation, UVA photons, or solar simulated light is critically reviewed. PMID:23378590

  11. DNA Base Damage by Reactive Oxygen Species, Oxidizing Agents, and UV Radiation

    PubMed Central

    Cadet, Jean; Wagner, J. Richard

    2013-01-01

    Emphasis has been placed in this article dedicated to DNA damage on recent aspects of the formation and measurement of oxidatively generated damage in cellular DNA in order to provide a comprehensive and updated survey. This includes single pyrimidine and purine base lesions, intrastrand cross-links, purine 5′,8-cyclonucleosides, DNA–protein adducts and interstrand cross-links formed by the reactions of either the nucleobases or the 2-deoxyribose moiety with the hydroxyl radical, one-electron oxidants, singlet oxygen, and hypochlorous acid. In addition, recent information concerning the mechanisms of formation, individual measurement, and repair-rate assessment of bipyrimidine photoproducts in isolated cells and human skin upon exposure to UVB radiation, UVA photons, or solar simulated light is critically reviewed. PMID:23378590

  12. Resonance-enhanced two-photon ionization of helium using an Ar{sup +} mode-locked laser synchronized with VUV synchrotron radiation pulses

    SciTech Connect

    Lacoursiere, Jean; Meyer, Michael; Nahon, Laurent; Morin, Paul; Larzilliere, Michel

    1995-04-01

    We report a new experimental set-up consisting in the synchronization of 74.9094 MHz pulses from a mode-locked Ar{sup +} laser with 8.32 MHz pulses of vacuum ultraviolet (VUV) synchrotron radiation from the Super-ACO storage ring of the Laboratorie pour l'Utilisation du Rayonnement Electromagnetique (LURE). The capabilities of the set-up are demonstrated in a time-resolved pump-probe (VUV+visible) experiment in which free helium atoms are resonantly ionized via the short-lived 1s3p ({sup 1}P) state. This experiment allowed us to show the relevance of this technique for the investigation of nanosecond dynamics on gas phase species.

  13. Resonance-enhanced two-photon ionization of helium using an Ar+ mode-locked laser synchronized with VUV synchrotron radiation pulses

    NASA Astrophysics Data System (ADS)

    Lacoursière, Jean; Meyer, Michael; Nahon, Laurent; Morin, Paul; Larzillière, Michel

    1995-04-01

    We report a new experimental set-up consisting in the synchronization of 74.9094 MHz pulses from a mode-locked Ar+ laser with 8.32 MHz pulses of vacuum ultraviolet (VUV) synchrotron radiation from the Super-ACO storage ring of the Laboratorie pour l'Utilisation du Rayonnement Electromagnetique (LURE). The capabilities of the set-up are demonstrated in a time-resolved pump-probe (VUV+visible) experiment in which free helium atoms are resonantly ionized via the short-lived 1s3p (1P) state. This experiment allowed us to show the relevance of this technique for the investigation of nanosecond dynamics on gas phase species.

  14. Time-resolved pump-probe photoelectron spectroscopy of helium using a mode-locked laser synchronized with synchrotron radiation pulses

    NASA Astrophysics Data System (ADS)

    Lacoursière, Jean; Meyer, Michael; Nahon, Laurent; Morin, Paul; Larzillière, Michel

    1994-12-01

    We report a new experimental setup consisting in the synchronization of 74.9094 MHz pulses from a mode-locked Ar + laser with 8.32 MHz pulses of vacuum ultraviolet (VUV) synchrotron radiation from the Super-ACO storage ring of the Laboratoire pour l'Utilisation du Rayonnement Electromagnétique (LURE). The capabilities of the setup are demonstrated in a time-resolved pump-probe (VUV + visible) experiment in which free helium atoms are resonantly ionized via the short-lived 1s3p ( 1P) state. This experiment allowed us to obtain the value of 1.12 ns for the temporal resolution (FWHM) of the experimental setup and to show the relevance of this technique for the investigation of nanosecond dynamics on gas phase species.

  15. Measurement of the helium 23S metastable atom density by observation of the change in the 23S-23P emission line shape due to radiation reabsorption

    NASA Astrophysics Data System (ADS)

    Shikama, T.; Ogane, S.; Iida, Y.; Hasuo, M.

    2016-01-01

    In helium discharge plasmas, the relative emission intensities of the fine-structure transitions belonging to the HeI 23S-23P transition can be affected by radiation reabsorption. Since the magnitude of the reabsorption depends on the density and temperature of the 23S metastable atoms, their density can be determined by measuring the 23S-23P emission line shape using a high wavelength-resolution spectrometer. In this study, the applicable conditions of the method in terms of the opacity and line broadening are revealed, and possible causes of errors in the measurement, i.e. spatial distributions of the density and temperature and the effects of external magnetic and electric fields, are investigated. The effect of reabsorption under an external magnetic field is experimentally confirmed using a glow discharge plasma installed in a superconducting magnet.

  16. Oscillator strengths and radiative decay rates for spin-changing S-P transitions in helium: finite nuclear mass effects

    NASA Astrophysics Data System (ADS)

    Morton, Donald C.; Schulhoff, Eva E.; Drake, G. W. F.

    2015-12-01

    We have calculated the electric dipole (E1) and magnetic quadrupole (M2) oscillator strengths and spontaneous decay rates for 24 spin-changing transitions of atomic helium. We included the effects of the finite nuclear mass and the anomalous magnetic moment of the electron augmented by the recently derived Pachucki term. The specific transitions for 4He are n{ }1{{{S}}}0-{n}\\prime { }3{{{P}}}{1,2} and n{ }3{{{S}}}1-{n}\\prime { }1{{{P}}}1 with n,{n}\\prime ≤slant 3 and n≤slant 10 for {n}\\prime =n. For the E1 calculations we used the Breit approximation and pseudostate expansions to perform the perturbation sums over intermediate states in both the length and velocity gauge as a check on both numerical accuracy and validity of the transition operators. The corrections for the nuclear mass and the electron anomaly tend to cancel, indicating that if one is included, then so should be the other. The tables give mass- and anomaly-dependent coefficients permitting the easy generation of results for the other isotopes of helium.

  17. Electrode level Monte Carlo model of radiation damage effects on astronomical CCDs

    NASA Astrophysics Data System (ADS)

    Prod'homme, T.; Brown, A. G. A.; Lindegren, L.; Short, A. D. T.; Brown, S. W.

    2011-07-01

    Current optical space telescopes rely upon silicon charge-coupled devices (CCDs) to detect and image the incoming photons. The performance of a CCD detector depends on its ability to transfer electrons through the silicon efficiently, so that the signal from every pixel may be read out through a single amplifier. This process of electron transfer is highly susceptible to the effects of solar proton damage (or non-ionizing radiation damage). This is because charged particles passing through the CCD displace silicon atoms, introducing energy levels into the semiconductor band gap which act as localized electron traps. The reduction in charge transfer efficiency (CTE) leads to signal loss and image smearing. The European Space Agency's astrometric Gaia mission will make extensive use of CCDs to create the most complete and accurate stereoscopic map to date of the Milky Way. In the context of the Gaia mission CTE is referred to with the complementary quantity charge transfer inefficiency (CTI = 1-CTE). CTI is an extremely important issue that threatens Gaia's performances: the CCDs are very large so that the electrons need to be transferred a long way; the focal plane is also very large and difficult to shield; the mission will operate at second Lagrange point where the direct solar protons are highly energetic (penetrating) and the science requirements on image quality are very stringent. In order to tackle this issue, in depth experimental studies and modelling efforts are being conducted to explore the possible consequences and to mitigate the anticipated effects of radiation damage. We present here a detailed Monte Carlo model that has been developed to simulate the operation of a damaged CCD at the pixel electrode level. This model implements a new approach to both the charge density distribution within a pixel and the charge capture and release probabilities, which allows the reproduction of CTI effects on a variety of measurements for a large signal level range

  18. Quantification of actinide alpha-radiation damage in minerals and ceramics.

    PubMed

    Farnan, Ian; Cho, Herman; Weber, William J

    2007-01-11

    There are large amounts of heavy alpha-emitters in nuclear waste and nuclear materials inventories stored in various sites around the world. These include plutonium and minor actinides such as americium and curium. In preparation for geological disposal there is consensus that actinides that have been separated from spent nuclear fuel should be immobilized within mineral-based ceramics rather than glass because of their superior aqueous durability and lower risk of accidental criticality. However, in the long term, the alpha-decay taking place in these ceramics will severely disrupt their crystalline structure and reduce their durability. A fundamental property in predicting cumulative radiation damage is the number of atoms permanently displaced per alpha-decay. At present, this number is estimated to be 1,000-2,000 atoms/alpha in zircon. Here we report nuclear magnetic resonance, spin-counting experiments that measure close to 5,000 atoms/alpha in radiation-damaged natural zircons. New radiological nuclear magnetic resonance measurements on highly radioactive, 239Pu zircon show damage similar to that caused by 238U and 232Th in mineral zircons at the same dose, indicating no significant effect of half-life or loading levels (dose rate). On the basis of these measurements, the initially crystalline structure of a 10 weight per cent 239Pu zircon would be amorphous after only 1,400 years in a geological repository (desired immobilization timescales are of the order of 250,000 years). These measurements establish a basis for assessing the long-term structural durability of actinide-containing ceramics in terms of an atomistic understanding of the fundamental damage event. PMID:17215840

  19. Exploiting radiation damage control on apatite (U Th)/He dates in cratonic regions

    NASA Astrophysics Data System (ADS)

    Flowers, Rebecca M.

    2009-01-01

    Apatites from four pairs of samples of Precambrian basement from the western Canadian shield were analyzed by (U-Th)/He thermochronometry to test for the influence of radiation damage on apatite (U-Th)/He dates in this cratonic region. Recent studies have demonstrated that the accumulation of radiation damage increases the apatite He retentivity, so that apatites with a span of effective U concentrations, eU, that experienced the same thermal history may be characterized by a range of closure temperatures. In this investigation, each sample pair consisted of a mafic dike cross-cutting felsic gneisses from a single outcrop or nearby outcrops that contained apatites with a span of eU. The apatites yielded (U-Th)/He dates from 846 to 123 Ma, and were positively correlated with eU within each sample pair. These results can be explained using a model that tracks the evolution of He mobility in response to the accumulation of radiation damage. When coupled with regional geological constraints, the data appear to require partial to complete He loss due to burial and reheating in Phanerozoic time. New apatite fission- track dates and length data were obtained for five of these samples. The apatite fission- track dates are Proterozoic regardless of apatite eU. Thermal history simulations indicate that the apatite fission-track data are compatible with the (U-Th)/He results, although the thermal histories are not identical in detail and the fission-track results alone do not require Phanerozoic heating. Together the data are consistent with burial of this region by ≥ 1 km of Phanerozoic strata that were subsequently denuded, thus pointing toward significant Phanerozoic deposition in the North American cratonic interior hundreds of kilometers east of where previously documented. The results suggest that exploiting radiation damage control on apatite (U-Th)/He dates through investigation of surface sample apatites with a span of closure temperatures can impose tighter

  20. Large-scale transient sensitivity analysis of a radiation damaged bipolar junction transistor.

    SciTech Connect

    Hoekstra, Robert John; Gay, David M.; Bartlett, Roscoe Ainsworth; Phipps, Eric Todd

    2007-11-01

    Automatic differentiation (AD) is useful in transient sensitivity analysis of a computational simulation of a bipolar junction transistor subject to radiation damage. We used forward-mode AD, implemented in a new Trilinos package called Sacado, to compute analytic derivatives for implicit time integration and forward sensitivity analysis. Sacado addresses element-based simulation codes written in C++ and works well with forward sensitivity analysis as implemented in the Trilinos time-integration package Rythmos. The forward sensitivity calculation is significantly more efficient and robust than finite differencing.

  1. Neutron Fluences and Radiation Damage Parameters for the HFIR-MFE-RB-17J Experiment

    SciTech Connect

    Greenwood, Lawrence R.; Glasgow, David C.; Baldwin, Charles A.

    2010-08-23

    The HFIR-MFE-RB-17J experiment was conducted in the removable beryllium (RB) position of HFIR with a Eu2O2 shield. The irradiation was conducted from April 27, 2004, to May 18, 2005. The total exposure was for 353.6 FPD (full power days). Reactor dosimetry capsules were analyzed and the activation data were used to provide the best estimates of the neutron fluences and radiation damage parameters as a function of height relative to midplane of the reactor.

  2. Radiation damage studies of cerium-doped radiation-resistant lead glass detectors

    NASA Astrophysics Data System (ADS)

    Adams, M. R.; Engelmann, R.; Grannis, P. D.; Horstkotte, J.; Godfrey, L.; Linn, S. L.; Marx, M. D.; Timms, J.; Tuts, P. M.; Willins, J.; Ahrens, L.; Aronson, S.; Levy, P. W.; Yamin, P.; Franzini, P.; Youssef, S.; Cutts, D.; Callas, J.

    1985-08-01

    Optical absorption measurements have been made on unirradiated and irradiated samples of cerium oxide doped lead silicate particle detector glasses. The addition of CeO 2 introduces one prominent and one weak absorption band near the ultraviolet transmission limit. However the CeO 2 greatly reduces the rate of formation of the radiation induced absorption. Detector size blocks of lead glass with 0.0, 0.25, and 1.2 weight percent CeO 2 were used to determine the energy resolution for 5 GeV electrons. The resolution deteriorates from 12 to 33% as the CeO 2 changes from 0.0 to 1.2%. At these CeO 2 levels, calculations indicate the yield of doped glass becomes superior to that of undoped glass for doses greater than 500 rad.

  3. Space Radiation Effects on Human Cells: Modeling DNA Breakage, DNA Damage Foci Distribution, Chromosomal Aberrations and Tissue Effects

    NASA Technical Reports Server (NTRS)

    Ponomarev, A. L.; Huff, J. L.; Cucinotta, F. A.

    2011-01-01

    Future long-tem space travel will face challenges from radiation concerns as the space environment poses health risk to humans in space from radiations with high biological efficiency and adverse post-flight long-term effects. Solar particles events may dramatically affect the crew performance, while Galactic Cosmic Rays will induce a chronic exposure to high-linear-energy-transfer (LET) particles. These types of radiation, not present on the ground level, can increase the probability of a fatal cancer later in astronaut life. No feasible shielding is possible from radiation in space, especially for the heavy ion component, as suggested solutions will require a dramatic increase in the mass of the mission. Our research group focuses on fundamental research and strategic analysis leading to better shielding design and to better understanding of the biological mechanisms of radiation damage. We present our recent effort to model DNA damage and tissue damage using computational models based on the physics of heavy ion radiation, DNA structure and DNA damage and repair in human cells. Our particular area of expertise include the clustered DNA damage from high-LET radiation, the visualization of DSBs (DNA double strand breaks) via DNA damage foci, image analysis and the statistics of the foci for different experimental situations, chromosomal aberration formation through DSB misrepair, the kinetics of DSB repair leading to a model-derived spectrum of chromosomal aberrations, and, finally, the simulation of human tissue and the pattern of apoptotic cell damage. This compendium of theoretical and experimental data sheds light on the complex nature of radiation interacting with human DNA, cells and tissues, which can lead to mutagenesis and carcinogenesis later in human life after the space mission.

  4. Radiation damage and annealing of lithium-doped silicon solar cells

    NASA Technical Reports Server (NTRS)

    Statler, R. L.

    1971-01-01

    Evidence has been presented that a lithium-diffused crucible-grown silicon solar cell can be made with better efficiency than the flight-quality n p 10 ohms-cm solar cell. When this lithium cell is exposed to a continuous radiation evironment at 60 C (electron spectrum from gamma rays) it has a higher power output than the N/P cell after a fluence equivalent to 1 MeV. A comparison of annealing of proton- and electron-damage in this lithium cell reveals a decidedly faster rate of recovery and higher level of recoverable power from the proton effects. Therefore, the lithium cell shows a good potential for many space missions where the proton flux is a significant fraction of the radiation field to be encountered.

  5. Ultraviolet radiation effects on the infrared damage rate of a thermal control coating

    NASA Technical Reports Server (NTRS)

    Bass, J. A.

    1972-01-01

    The effects of ultraviolet radiation on the infrared reflectance of ZnO silicone white thermal coatings were investigated. Narrow band ultraviolet radiation for wavelengths in the 2200A to 3500A range by a monochromator and a high pressure, 150-W Eimac xenon lamp. The sample was irradiated while in a vacuum of at least 0.000001 torr, and infrared reflectance was measured in situ with a spectroreflectometer at 19,500A. Reflectance degradation was studied as a function of wavelength, time, intensity, and dose. Damage was wavelength dependent at constant exposure, but no maximum was evident above the shortest wavelength investigated here. The degradation rate at constant intensity was an exponential function of time and varies with intensity.

  6. Infrared response measurements on radiation-damaged Si/Li/ detectors.

    NASA Technical Reports Server (NTRS)

    Sher, A. H.; Liu, Y. M.; Keery, W. J.

    1972-01-01

    The improved infrared response (IRR) technique has been used to qualitatively compare radiation effects on Si(Li) detectors with energy levels reported for silicon in the literature. Measurements have been made on five commercial silicon detectors and one fabricated in-house, both before and after irradiation with fast neutrons, 1.9-MeV protons, and 1.6-MeV electrons. Effects dependent upon the extent of radiation damage have been observed. It seems likely that the photo-EMF, or photo-voltage, effect is the basic mechanism for the observation of IRR in p-i-n diodes with a wide i-region. Experimental characteristics of the IRR measurement are in agreement with those of the photovoltage effect.

  7. DETECTION OF LOW DOSE RADIATION-AND CHEMICALLY-INDUCED DNA DAMAGE USING TEMPERATURE DIFFERENTIAL FLUORESCENCE ASSAYS

    EPA Science Inventory

    Rapid, sensitive and simple assays for radiation- and chemically-induced DNA damage can be of significant benefit to a number of fields including radiation biology, clinical research, and environmental monitoring. Although temperature-induced DNA strand separation has been use...

  8. Debris and radiation-induced damage effects on EUV nanolithography source collector mirror optics performance.

    SciTech Connect

    Allain, J. P.; Nieto, M.; Hendricks, M.; Harilal, S. S.; Hassanein, A.; Mathematics and Computer Science

    2007-01-01

    Exposure of collector mirrors facing the hot, dense pinch plasma in plasma-based EUV light sources to debris (fast ions, neutrals, off-band radiation, droplets) remains one of the highest critical issues of source component lifetime and commercial feasibility of nanolithography at 13.5-nm. Typical radiators used at 13.5-nm include Xe and Sn. Fast particles emerging from the pinch region of the lamp are known to induce serious damage to nearby collector mirrors. Candidate collector configurations include either multi-layer mirrors (MLM) or single-layer mirrors (SLM) used at grazing incidence. Studies at Argonne have focused on understanding the underlying mechanisms that hinder collector mirror performance at 13.5-nm under fast Sn or Xe exposure. This is possible by a new state-of-the-art in-situ EUV reflectometry system that measures real time relative EUV reflectivity (15-degree incidence and 13.5-nm) variation during fast particle exposure. Intense EUV light and off-band radiation is also known to contribute to mirror damage. For example offband radiation can couple to the mirror and induce heating affecting the mirror's surface properties. In addition, intense EUV light can partially photo-ionize background gas (e.g., Ar or He) used for mitigation in the source device. This can lead to local weakly ionized plasma creating a sheath and accelerating charged gas particles to the mirror surface and inducing sputtering. In this paper we study several aspects of debris and radiation-induced damage to candidate EUVL source collector optics materials. The first study concerns the use of IMD simulations to study the effect of surface roughness on EUV reflectivity. The second studies the effect of fast particles on MLM reflectivity at 13.5-nm. And lastly the third studies the effect of multiple energetic sources with thermal Sn on 13.5-nm reflectivity. These studies focus on conditions that simulate the EUVL source environment in a controlled way.

  9. Pudendal Nerve and Internal Pudendal Artery Damage May Contribute to Radiation-Induced Erectile Dysfunction

    SciTech Connect

    Nolan, Michael W.; Marolf, Angela J.; Ehrhart, E.J.; Rao, Sangeeta; Kraft, Susan L.; Engel, Stephanie; Yoshikawa, Hiroto; Golden, Anne E.; Wasserman, Todd H.; LaRue, Susan M.

    2015-03-15

    Purpose/Objectives: Erectile dysfunction is common after radiation therapy for prostate cancer; yet, the etiopathology of radiation-induced erectile dysfunction (RI-ED) remains poorly understood. A novel animal model was developed to study RI-ED, wherein stereotactic body radiation therapy (SBRT) was used to irradiate the prostate, neurovascular bundles (NVB), and penile bulb (PB) of dogs. The purpose was to describe vascular and neurogenic injuries after the irradiation of only the NVB or the PB, and after irradiation of all 3 sites (prostate, NVB, and PB) with varying doses of radiation. Methods and Materials: Dogs were treated with 50, 40, or 30 Gy to the prostate, NVB, and PB, or 50 Gy to either the NVB or the PB, by 5-fraction SBRT. Electrophysiologic studies of the pudendal nerve and bulbospongiosus muscles and ultrasound studies of pelvic perfusion were performed before and after SBRT. The results of these bioassays were correlated with histopathologic changes. Results: SBRT caused slowing of the systolic rise time, which corresponded to decreased arterial patency. Alterations in the response of the internal pudendal artery to vasoactive drugs were observed, wherein SBRT caused a paradoxical response to papaverine, slowing the systolic rise time after 40 and 50 Gy; these changes appeared to have some dose dependency. The neurofilament content of penile nerves was also decreased at high doses and was more profound when the PB was irradiated than when the NVB was irradiated. These findings are coincident with slowing of motor nerve conduction velocities in the pudendal nerve after SBRT. Conclusions: This is the first report in which prostatic irradiation was shown to cause morphologic arterial damage that was coincident with altered internal pudendal arterial tone, and in which decreased motor function in the pudendal nerve was attributed to axonal degeneration and loss. Further investigation of the role played by damage to these structures in RI-ED is

  10. Involvement of inducible nitric oxide synthase in radiation-induced vascular endothelial damage.

    PubMed

    Hong, Chang-Won; Kim, Young-Mee; Pyo, Hongryull; Lee, Joon-Ho; Kim, Suwan; Lee, Sunyoung; Noh, Jae Myoung

    2013-11-01

    The use of radiation therapy has been linked to an increased risk of cardiovascular disease. To understand the mechanisms underlying radiation-induced vascular dysfunction, we employed two models. First, we examined the effect of X-ray irradiation on vasodilation in rabbit carotid arteries. Carotid arterial rings were irradiated with 8 or 16 Gy using in vivo and ex vivo methods. We measured the effect of acetylcholine-induced relaxation after phenylephrine-induced contraction on the rings. In irradiated carotid arteries, vasodilation was significantly attenuated by both irradiation methods. The relaxation response was completely blocked by 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, a potent inhibitor of soluble guanylate cyclase. Residual relaxation persisted after treatment with L-N(ω)-nitroarginine (L-NA), a non-specific inhibitor of nitric oxide synthase (NOS), but disappeared following the addition of aminoguanidine (AG), a selective inhibitor of inducible NOS (iNOS). The relaxation response was also affected by tetraethylammonium, an inhibitor of endothelium-derived hyperpolarizing factor activity. In the second model, we investigated the biochemical events of nitrosative stress in human umbilical-vein endothelial cells (HUVECs). We measured iNOS and nitrotyrosine expression in HUVECs exposed to a dose of 4 Gy. The expression of iNOS and nitrotyrosine was greater in irradiated HUVECs than in untreated controls. Pretreatment with AG, L-N(6)-(1-iminoethyl) lysine hydrochloride (a selective inhibitor of iNOS), and L-NA attenuated nitrosative stress. While a selective target of radiation-induced vascular endothelial damage was not definitely determined, these results suggest that NO generated from iNOS could contribute to vasorelaxation. These studies highlight a potential role of iNOS inhibitors in ameliorating radiation-induced vascular endothelial damage. PMID:23704776

  11. Anisotropic mechanical properties of zircon and the effect of radiation damage

    NASA Astrophysics Data System (ADS)

    Beirau, Tobias; Nix, William D.; Bismayer, Ulrich; Boatner, Lynn A.; Isaacson, Scott G.; Ewing, Rodney C.

    2016-06-01

    This study provides new insights into the relationship between radiation-dose-dependent structural damage due to natural U and Th impurities and the anisotropic mechanical properties (Poisson's ratio, elastic modulus and hardness) of zircon. Natural zircon samples from Sri Lanka (see Muarakami et al. in Am Mineral 76:1510-1532, 1991) and synthetic samples, covering a dose range of zero up to 6.8 × 1018 α-decays/g, have been studied by nanoindentation. Measurements along the [100] crystallographic direction and calculations, based on elastic stiffness constants determined by Özkan (J Appl Phys 47:4772-4779, 1976), revealed a general radiation-induced decrease in stiffness (~54 %) and hardness (~48 %) and an increase in the Poisson's ratio (~54 %) with increasing dose. Additional indentations on selected samples along the [001] allowed one to follow the amorphization process to the point that the mechanical properties are isotropic. This work shows that the radiation-dose-dependent changes of the mechanical properties of zircon can be directly correlated with the amorphous fraction as determined by previous investigations with local and global probes (Ríos et al. in J Phys Condens Matter 12:2401-2412, 2000a; Farnan and Salje in J Appl Phys 89:2084-2090, 2001; Zhang and Salje in J Phys Condens Matter 13:3057-3071, 2001). The excellent agreement, revealed by the different methods, indicates a large influence of structural and even local phenomena on the macroscopic mechanical properties. Therefore, this study indicates the importance of acquiring better knowledge about the mechanical long-term stability of radiation-damaged materials.

  12. Cell damage by UVA radiation of a mercury microscopy lamp probed by autofluorescence modifications, cloning assay, and comet assay

    NASA Astrophysics Data System (ADS)

    Koenig, Karsten; Krasieva, Tatiana B.; Bauer, Eckhard; Fiedler, Ursula; Berns, Michael W.; Tromberg, Bruce J.; Greulich, Karl O.

    1996-04-01

    Cell damage by low-power 365-nm radiation of a 50-W high-pressure mercury microscopy lamp was studied. Exposure of Chinese hamster ovary cells to ultraviolet-A (UVA) radiation > 10 kJ/m2 resulted in significant modifications of nicotinamide adenine dinucleotide attributed autofluorescence and inhibition of cell division. Single-cell gel electrophoresis (comet assay) revealed UVA-induced single-strand DNA breaks. According to these results, UVA excitation radiation in fluorescence microscopy may damage cells. This has to be considered in vital cell microscopy, e.g., in calcium measurements.

  13. Differential protection by nitroxides and hydroxylamines to radiation-induced and metal ion-catalyzed oxidative damage.

    PubMed

    Xavier, Sandhya; Yamada, Ken-ichi; Samuni, Ayelet M; Samuni, Amram; DeGraff, William; Krishna, Murali C; Mitchell, James B

    2002-11-14

    Modulation of radiation- and metal ion-catalyzed oxidative-induced damage using plasmid DNA, genomic DNA, and cell survival, by three nitroxides and their corresponding hydroxylamines, were examined. The antioxidant property of each compound was independently determined by reacting supercoiled DNA with copper II/1,10-phenanthroline complex fueled by the products of hypoxanthine/xanthine oxidase (HX/XO) and noting the protective effect as assessed by agarose gel electrophoresis. The nitroxides and their corresponding hydroxylamines protected approximately to the same degree (33-47% relaxed form) when compared to 76.7% relaxed form in the absence of protectors. Likewise, protection by both the nitroxide and corresponding hydroxylamine were observed for Chinese hamster V79 cells exposed to hydrogen peroxide. In contrast, when plasmid DNA damage was induced by ionizing radiation (100 Gy), only nitroxides (10 mM) provide protection (32.4-38.5% relaxed form) when compared to radiation alone or in the presence of hydroxylamines (10 mM) (79.8% relaxed form). Nitroxide protection was concentration dependent. Radiation cell survival studies and DNA double-strand break (DBS) assessment (pulse field electrophoresis) showed that only the nitroxide protected or prevented damage, respectively. Collectively, the results show that nitroxides and hydroxylamines protect equally against the damage mediated by oxidants generated by the metal ion-catalyzed Haber-Weiss reaction, but only nitroxides protect against radiation damage, suggesting that nitroxides may more readily react with intermediate radical species produced by radiation than hydroxylamines. PMID:12399020

  14. Scintillation mechanism and radiation damage in Ce{sub x}La{sub 1-x}F{sub 3} crystals

    SciTech Connect

    Wojtowicz, A.J.; Wisniewski, D. |; Lempicki, A.; Brecher, C.; Bartram, R.H.; Woody, C.; Levy, P.; Stoll, S.; Kierstead, J.; Pedrini, C.

    1994-08-01

    Recent spectroscopic and radiation damage experiments on a series of Ce{sub x}La{sub 1{minus}x}F{sub 3} crystals suggest that the scintillation light output is limited by an unusual quenching mechanism, which also plays a major role in minimizing radiation-induced damage. The intensity of the radiation-induced absorptions is a strong function of the Ce content x, reaching a maximum for x = 0.03 and a minimum for x = 1. This peculiar dependence appears to be due to the influence of deep-lying Ce levels on both scintillation mechanism and radiation damage. The authors suggest that various charge transfer processes can explain many aspects of the performance of Ce{sub x}La{sub 1{minus}x}F{sub 3} scintillators.

  15. Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation

    PubMed Central

    Amaro-Ortiz, Alexandra; Yan, Betty; D’Orazio, John A.

    2015-01-01

    Being the largest and most visible organ of the body and heavily influenced by environmental factors, skin is ideal to study long-term effects of aging. Throughout our lifetime, we accumulate damage generated by UV radiation. UV causes inflammation, immune changes, physical changes, impaired wound healing and DNA damage that promotes cellular senescence and carcinogenesis. Melanoma is the deadliest form of skin cancer and among the malignancies of highest increasing incidence over the last several decades. Melanoma incidence is directly related to age, with highest rates in individuals over the age of 55 years, making it a clear age-related disease. In this review, we will focus on UV-induced carcinogenesis and photo aging along with natural protective mechanisms that reduce amount of “realized” solar radiation dose and UV-induced injury. We will focus on the theoretical use of forskolin, a plant-derived pharmacologically active compound to protect the skin against UV injury and prevent aging symptoms by up-regulating melanin production. We will discuss its use as a topically-applied root-derived formulation of the Plectranthus barbatus (Coleus forskolii) plant that grows naturally in Asia and that has long been used in various Aryuvedic teas and therapeutic preparations. PMID:24838074

  16. Modelling electron distributions within ESA's Gaia satellite CCD pixels to mitigate radiation damage

    NASA Astrophysics Data System (ADS)

    Seabroke, G. M.; Holland, A. D.; Burt, D.; Robbins, M. S.

    2009-08-01

    The Gaia satellite is a high-precision astrometry, photometry and spectroscopic ESA cornerstone mission, currently scheduled for launch in 2012. Its primary science drivers are the composition, formation and evolution of the Galaxy. Gaia will achieve its unprecedented positional accuracy requirements with detailed calibration and correction for radiation damage. At L2, protons cause displacement damage in the silicon of CCDs. The resulting traps capture and emit electrons from passing charge packets in the CCD pixel, distorting the image PSF and biasing its centroid. Microscopic models of Gaia's CCDs are being developed to simulate this effect. The key to calculating the probability of an electron being captured by a trap is the 3D electron density within each CCD pixel. However, this has not been physically modelled for the Gaia CCD pixels. In Seabroke, Holland & Cropper (2008), the first paper of this series, we motivated the need for such specialised 3D device modelling and outlined how its future results will fit into Gaia's overall radiation calibration strategy. In this paper, the second of the series, we present our first results using Silvaco's physics-based, engineering software: the ATLAS device simulation framework. Inputting a doping profile, pixel geometry and materials into ATLAS and comparing the results to other simulations reveals that ATLAS has a free parameter, fixed oxide charge, that needs to be calibrated. ATLAS is successfully benchmarked against other simulations and measurements of a test device, identifying how to use it to model Gaia pixels and highlighting the affect of different doping approximations.

  17. The contribution made by lattice vacancies to the Wigner effect in radiation-damaged graphite.

    PubMed

    Latham, C D; Heggie, M I; Alatalo, M; Oberg, S; Briddon, P R

    2013-04-01

    Models for radiation damage in graphite are reviewed and compared, leading to a re-examination of the contribution made by vacancies to annealing processes. A method based on density functional theory, using large supercells with orthorhombic and hexagonal symmetry, is employed to calculate the properties and behaviour of lattice vacancies and displacement defects. It is concluded that annihilation of intimate Frenkel defects marks the onset of recovery in electrical resistivity, which occurs when the temperature exceeds about 160 K. The migration of isolated monovacancies is estimated to have an activation energy of E(a) ≈ 1.1 eV. Coalescence into divacancy defects occurs in several stages, with different barriers at each stage, depending on the path. The formation of pairs ultimately yields up to 8.9 eV energy, which is nearly 1.0 eV more than the formation energy for an isolated monovacancy. Processes resulting in vacancy coalescence and annihilation appear to be responsible for the main Wigner energy release peak in radiation-damaged graphite, occurring at about 475 K. PMID:23470497

  18. The contribution made by lattice vacancies to the Wigner effect in radiation-damaged graphite

    NASA Astrophysics Data System (ADS)

    Latham, C. D.; Heggie, M. I.; Alatalo, M.; Öberg, S.; Briddon, P. R.

    2013-04-01

    Models for radiation damage in graphite are reviewed and compared, leading to a re-examination of the contribution made by vacancies to annealing processes. A method based on density functional theory, using large supercells with orthorhombic and hexagonal symmetry, is employed to calculate the properties and behaviour of lattice vacancies and displacement defects. It is concluded that annihilation of intimate Frenkel defects marks the onset of recovery in electrical resistivity, which occurs when the temperature exceeds about 160 K. The migration of isolated monovacancies is estimated to have an activation energy of Ea ≈ 1.1 eV. Coalescence into divacancy defects occurs in several stages, with different barriers at each stage, depending on the path. The formation of pairs ultimately yields up to 8.9 eV energy, which is nearly 1.0 eV more than the formation energy for an isolated monovacancy. Processes resulting in vacancy coalescence and annihilation appear to be responsible for the main Wigner energy release peak in radiation-damaged graphite, occurring at about 475 K.

  19. Effects of Ionizing Radiation on Biological Molecules—Mechanisms of Damage and Emerging Methods of Detection

    PubMed Central

    Reisz, Julie A.; Bansal, Nidhi; Qian, Jiang; Zhao, Weiling

    2014-01-01

    Abstract Significance: The detrimental effects of ionizing radiation (IR) involve a highly orchestrated series of events that are amplified by endogenous signaling and culminating in oxidative damage to DNA, lipids, proteins, and many metabolites. Despite the global impact of IR, the molecular mechanisms underlying tissue damage reveal that many biomolecules are chemoselectively modified by IR. Recent Advances: The development of high-throughput “omics” technologies for mapping DNA and protein modifications have revolutionized the study of IR effects on biological systems. Studies in cells, tissues, and biological fluids are used to identify molecular features or biomarkers of IR exposure and response and the molecular mechanisms that regulate their expression or synthesis. Critical Issues: In this review, chemical mechanisms are described for IR-induced modifications of biomolecules along with methods for their detection. Included with the detection methods are crucial experimental considerations and caveats for their use. Additional factors critical to the cellular response to radiation, including alterations in protein expression, metabolomics, and epigenetic factors, are also discussed. Future Directions: Throughout the review, the synergy of combined “omics” technologies such as genomics and epigenomics, proteomics, and metabolomics is highlighted. These are anticipated to lead to new hypotheses to understand IR effects on biological systems and improve IR-based therapies. Antioxid. Redox Signal. 21: 260–292. PMID:24382094

  20. High-energy proton radiation damage of high-purity germanium detectors

    NASA Technical Reports Server (NTRS)

    Pehl, R. H.; Varnell, L. S.; Metzger, A. E.

    1978-01-01

    Quantitative studies of radiation damage in high-purity germanium gamma-ray detectors due to high-energy charged particles have been carried out; two 1.0 cm thick planar detectors were irradiated by 6 GeV/c protons. Under proton bombardment, degradation in the energy resolution was found to begin below 7 x 10 to the 7th protons/sq cm and increased proportionately in both detectors until the experiment was terminated at a total flux of 5.7 x 10 to the 8th protons/sq cm, equivalent to about a six year exposure to cosmic-ray protons in space. At the end of the irradiation, the FWHM resolution measured at 1332 keV stood at 8.5 and 13.6 keV, with both detectors of only marginal utility as a spectrometer due to the severe tailing caused by charge trapping. Annealing these detectors after proton damage was found to be much easier than after neutron damage.

  1. Ozone depletion and UVB radiation: impact on plant DNA damage in southern South America.

    PubMed

    Rousseaux, M C; Ballaré, C L; Giordano, C V; Scopel, A L; Zima, A M; Szwarcberg-Bracchitta, M; Searles, P S; Caldwell, M M; Díaz, S B

    1999-12-21

    The primary motivation behind the considerable effort in studying stratospheric ozone depletion is the potential for biological consequences of increased solar UVB (280-315 nm) radiation. Yet, direct links between ozone depletion and biological impacts have been established only for organisms of Antarctic waters under the influence of the ozone "hole;" no direct evidence exists that ozone-related variations in UVB affect ecosystems of temperate latitudes. Indeed, calculations based on laboratory studies with plants suggest that the biological impact of ozone depletion (measured by the formation of cyclobutane pyrimidine dimers in DNA) is likely to be less marked than previously thought, because UVA quanta (315-400 nm) may also cause significant damage, and UVA is unaffected by ozone depletion. Herein, we show that the temperate ecosystems of southern South America have been subjected to increasingly high levels of ozone depletion during the last decade. We found that in the spring of 1997, despite frequent cloud cover, the passages of the ozone hole over Tierra del Fuego (55 degrees S) caused concomitant increases in solar UV and that the enhanced ground-level UV led to significant increases in DNA damage in the native plant Gunnera magellanica. The fluctuations in solar UV explained a large proportion of the variation in DNA damage (up to 68%), particularly when the solar UV was weighted for biological effectiveness according to action spectra that assume a sharp decline in quantum efficiency with increasing wavelength from the UVB into the UVA regions of the spectrum. PMID:10611381

  2. The damage repair role of He-Ne laser on plants exposed to different intensities of ultraviolet-B radiation.

    PubMed

    Qi, Zhi; Yue, Ming; Han, Rong; Wang, Xun-Ling

    2002-06-01

    Light-grown broad bean (Vicia faba L.) seedlings were subjected to different intensities of UV-B radiation (0, 0.05, 0.15, 0.45, 0.90, 1.45 and 1.98 W m(-2)) for 7 h under photosynthetically active radiation (70 micromol m(-2) s(-1)) and then exposed to He-Ne laser (632.8 nm, 5.43 mW mm(-2)) radiation for 5 min or red light radiation for 4 h without ambient light radiation. When He-Ne laser radiated leaves were treated using lower intensity UV-B, the activities of superoxide dismutase (EC 1.15.1.1), ascorbate peroxidase (EC 1.11.1.11) and catalase (EC 1.11.1.6) improved significantly. Moreover, the UV-B-injured plants treated with laser light recovered faster from UV-B treatment because the concentration of malondialdehyde and the rate of electrolyte leakage from leaf disks reached control levels (no UV-B or laser treatment) early compared with those exposed only to ambient light or in dark conditions. Laser treatment, however, had no repair effect on seedling damage induced by higher UV-B radiation (1.45 and 1.98 W m(-2)), even with higher laser flux rates and longer laser treatment. In addition, the red light treatment had no repair effect on UV-B-induced damage. Meanwhile, the long-term physiological effect of He-Ne laser treatment on UV-B damaged plants was presented and evaluated. The results showed that the laser had a long-term positive physiological effect on the growth of UV-B-damaged plants. With the exception of the severe damage caused by higher UV-B radiation, a laser with the proper flux rate and treatment time can repair UV-B-induced damage and shorten the recovery time. PMID:12081332

  3. Pharmaceutical drugs supporting regeneration of small-intestinal mucosa severely damaged by ionizing radiation in mice

    PubMed Central

    Ishihara, Hiroshi; Tanaka, Izumi; Yakumaru, Haruko; Tanaka, Mika; Yokochi, Kazuko; Akashi, Makoto

    2013-01-01

    Accidental exposure of the abdomen to high-dose radiation leads to severe consequences initiated by disruption of the mucosa in the small intestine. Therapeutic options are limited, even though various treatments have been investigated, particularly in the field of regenerative therapy. In order to identify readily available treatment methods, we included several current pharmaceutical drugs, for which the clinical trials have already been completed, in tests on mice that had undergone severe mucosal damage by radiation. The drugs were injected into mice 24 h after exposure to 15.7 Gy X-rays. The effects of the drugs on the damaged mucosa of the small intestine were evaluated using early regeneration indices [the expression of c-myb mRNA, and proliferation of epithelial cells in the form of microcolonies (MCs) by Days 4 and 5 post-irradiation] and the survival rate of the mice. Enhancement of mucosal regeneration at Day 4 (c-myb: P < 0.01, MC: P < 0.05) and improvement of the survival rate (P < 0.05) were observed when a clinical dose of gonadotropin, a stimulator of androgen, was injected. Similarly, a clinical dose of thiamazole (which prevents secretion of thyroid hormone) stimulated mucosal growth by Day 5 (c-myb: P < 0.01, MC: P < 0.05) and also improved the survival rate (P < 0.05). The nonclinical drugs histamine and high-dose octreotide (a growth hormone antagonist) also gave significant survival-enhancing benefits (P < 0.01 and P < 0.05, respectively). These results can be used to construct therapeutic programs and applied in various experimental studies to control the regeneration of damaged mucosa. PMID:23728323

  4. Diffusion of fission products and radiation damage in SiC

    NASA Astrophysics Data System (ADS)

    Malherbe, Johan B.

    2013-11-01

    A major problem with most of the present nuclear reactors is their safety in terms of the release of radioactivity into the environment during accidents. In some of the future nuclear reactor designs, i.e. Generation IV reactors, the fuel is in the form of coated spherical particles, i.e. TRISO (acronym for triple coated isotropic) particles. The main function of these coating layers is to act as diffusion barriers for radioactive fission products, thereby keeping these fission products within the fuel particles, even under accident conditions. The most important coating layer is composed of polycrystalline 3C-SiC. This paper reviews the diffusion of the important fission products (silver, caesium, iodine and strontium) in SiC. Because radiation damage can induce and enhance diffusion, the paper also briefly reviews damage created by energetic neutrons and ions at elevated temperatures, i.e. the temperatures at which the modern reactors will operate, and the annealing of the damage. The interaction between SiC and some fission products (such as Pd and I) is also briefly discussed. As shown, one of the key advantages of SiC is its radiation hardness at elevated temperatures, i.e. SiC is not amorphized by neutrons or bombardment at substrate temperatures above 350 °C. Based on the diffusion coefficients of the fission products considered, the review shows that at the normal operating temperatures of these new reactors (i.e. less than 950 °C) the SiC coating layer is a good diffusion barrier for these fission products. However, at higher temperatures the design of the coated particles needs to be adapted, possibly by adding a thin layer of ZrC.

  5. Accelerated radiation damage test facility using a 5 MV tandem ion accelerator

    NASA Astrophysics Data System (ADS)

    Wady, P. T.; Draude, A.; Shubeita, S. M.; Smith, A. D.; Mason, N.; Pimblott, S. M.; Jimenez-Melero, E.

    2016-01-01

    We have developed a new irradiation facility that allows to perform accelerated damage tests of nuclear reactor materials at temperatures up to 400 °C using the intense proton (<100 μA) and heavy ion (≈10 μA) beams produced by a 5 MV tandem ion accelerator. The dedicated beam line for radiation damage studies comprises: (1) beam diagnosis and focusing optical components, (2) a scanning and slit system that allows uniform irradiation of a sample area of 0.5-6 cm2, and (3) a sample stage designed to be able to monitor in-situ the sample temperature, current deposited on the sample, and the gamma spectrum of potential radio-active nuclides produced during the sample irradiation. The beam line capabilities have been tested by irradiating a 20Cr-25Ni-Nb stabilised stainless steel with a 3 MeV proton beam to a dose level of 3 dpa. The irradiation temperature was 356 °C, with a maximum range in temperature values of ±6 °C within the first 24 h of continuous irradiation. The sample stage is connected to ground through an electrometer to measure accurately the charge deposited on the sample. The charge can be integrated in hardware during irradiation, and this methodology removes uncertainties due to fluctuations in beam current. The measured gamma spectrum allowed the identification of the main radioactive nuclides produced during the proton bombardment from the lifetimes and gamma emissions. This dedicated radiation damage beam line is hosted by the Dalton Cumbrian Facility of the University of Manchester.

  6. Integrated Molecular Analysis Indicates Undetectable Change in DNA Damage in Mice after Continuous Irradiation at ~ 400-fold Natural Background Radiation

    PubMed Central

    Olipitz, Werner; Wiktor-Brown, Dominika; Shuga, Joe; Pang, Bo; McFaline, Jose; Lonkar, Pallavi; Thomas, Aline; Mutamba, James T; Greenberger, Joel S; Samson, Leona D; Dedon, Peter C; Yanch, Jacquelyn C

    2012-01-01

    Background: In the event of a nuclear accident, people are exposed to elevated levels of continuous low dose-rate radiation. Nevertheless, most of the literature describes the biological effects of acute radiation. Objectives: DNA damage and mutations are well established for their carcinogenic effects. We assessed several key markers of DNA damage and DNA damage responses in mice exposed to low dose-rate radiation to reveal potential genotoxic effects associated with low dose-rate radiation. Methods: We studied low dose-rate radiation using a variable low dose-rate irradiator consisting of flood phantoms filled with 125Iodine-containing buffer. Mice were exposed to 0.0002 cGy/min (~ 400-fold background radiation) continuously over 5 weeks. We assessed base lesions, micronuclei, homologous recombination (HR; using fluorescent yellow direct repeat mice), and transcript levels for several radiation-sensitive genes. Results: We did not observe any changes in the levels of the DNA nucleobase damage products hypoxanthine, 8-oxo-7,8-dihydroguanine, 1,N6-ethenoadenine, or 3,N4-ethenocytosine above background levels under low dose-rate conditions. The micronucleus assay revealed no evidence that low dose-rate radiation induced DNA fragmentation, and there was no evidence of double strand break–induced HR. Furthermore, low dose-rate radiation did not induce Cdkn1a, Gadd45a, Mdm2, Atm, or Dbd2. Importantly, the same total dose, when delivered acutely, induced micronuclei and transcriptional responses. Conclusions: These results demonstrate in an in vivo animal model that lowering the dose-rate suppresses the potentially deleterious impact of radiation and calls attention to the need for a deeper understanding of the biological impact of low dose-rate radiation. PMID:22538203

  7. Genetic compensation of high dose radiation-induced damage in an anhydrobiotic insect

    NASA Astrophysics Data System (ADS)

    Gusev, Oleg; Nakahara, Yuichi; Sakashita, Tetsuya; Kikawada, Takahiro; Okuda, Takashi

    Anhydrobiotic larvae of African chironomid Polypedilum vanderplanki are known to show an extremely high tolerance against a range of stresses. The tolerance against various extreme environments exhibited by that insect might be due to being almost completely desiccated replacing water with trehalose, a state where little or no chemical reactions occur. From 2005 dried larvae of this insect are being used in a number of space experiments, both inside and outside of ISS as a model organism for estimation the limits of higher organisms' resistance to space environment stresses and long-term storage of the alive anhydrobiotic organisms during continues spaceflight. We have shown previously that both hydrated and dried larvae of Polypedilum vanderplanki have very higher tolerance against both highand low-linear energy transfer (LET), surviving after 7000Gy irradiation. It was suggested that the larvae would have effective DNA-reparation system in addition to artificial protection provided by glass-stage without water. In the present study we conducted analysis of stress-related gene expression in the larvae after 70-2000 Gy irradiations. Both DNA damage level and activity of DNA-reparation, anti-apoptotic and protein-damage related genes were analyzed. Direct visualization of DNA damage in the larvae fat body cells using Comet Assay showed that fragmented by radiation DNA is re-arranged within 76-98 hours after exposure. We found that massive overexpression of hsp and anti-oxidant genes occur in larvae entering anhydrobiosis , and provides refolding of proteins after rehydration. In the irradiated larvae overexpression of DNA-reparation enzymes anti-apoptotic genes was confirmed, suggesting that survival after high-dose irradiation is a result of combination of highly effective blocking of entering the apoptosis after severe DNA damage and DNA reparation.

  8. Theoretical versus Ex Vivo Assessment of Radiation Damage Repair: An Investigation in Normal Breast Tissue.

    PubMed

    Ebert, Martin A; Dhal, Bipina; Prunster, Janelle; McLaren, Sally; Zeps, Nikolajs; House, Michael; Reniers, Brigitte; Verhaegen, Frank; Corica, Tammy; Saunders, Christobel; Joseph, David J

    2016-04-01

    In vivo validation of models of DNA damage repair will enable their use for optimizing clinical radiotherapy. In this study, a theoretical assessment was made of DNA double-strand break (DSB) induction in normal breast tissue after intraoperative radiation therapy (IORT), which is now an accepted form of adjuvant radiotherapy for selected patients with early breast cancer. DSB rates and relative biological effectiveness (RBE) were calculated as a function of dose, radiation quality and dose rate, each varying based on the applicator size used during IORT. The spectra of primary electrons in breast tissue adjacent to each applicator were calculated using measured X-ray spectra and Monte Carlo methods, and were used to inform a Monte Carlo damage simulation code. In the absence of repair, asymptotic RBE values (relative to (60)Co) were approximately 1.5. Beam-quality changes led to only minor variations in RBE among applicators, though differences in dose rate and overall dose delivery time led to larger variations and a rapid decrease in RBE. An experimental assessment of DSB induction was performed ex vivo using pre- and postirradiation tissue samples from patients receiving breast intraoperative radiation therapy. Relative DSB rates were assessed via γ-H2AX immunohistochemistry using proportional staining. Maximum-likelihood parameter estimation yielded a DSB repair halftime of 25.9 min (95% CI, 21.5-30.4 min), although the resulting model was not statistically distinguishable from one where there was no change in DSB yield among patients. Although the model yielded an in vivo repair halftime of the order of previous estimates for in vitro repair halftimes, we cannot conclude that it is valid in this context. This study highlights some of the uncertainties inherent in population analysis of ex vivo samples, and of the quantitative limitations of immunohistochemistry for assessment of DSB repair. PMID:27023258

  9. HSPB1 polymorphisms might be associated with radiation-induced damage risk in lung cancer patients treated with radiotherapy.

    PubMed

    Li, Xiaofeng; Xu, Sheng; Cheng, Yu; Shu, Jun

    2016-05-01

    Several studies investigating the association between heat shock protein beta-1 (HSPB1) polymorphisms and radiation-induced damage in lung cancer patients administrated with radiotherapy have derived conflicting results. This meta-analysis aimed to assess the association between the HSPB1 genes' (rs2868370 and rs2868371) polymorphisms and the risk of radiation-induced damage in lung cancer patients. After an electronic literature search, four articles including six studies were found to be eligible for this meta-analysis. No association was observed between rs2868370 genotypes and radiation-induced damage risk. However, rs2868371 showed a statistically increased risk of radiation-induced damage under CC vs. CG/GG model (OR = 1.59, 95 % CI = 1.10-2.29). Subgroup analysis by ethnicity showed that the genotypes of rs2868371 were also associated with a significantly increased risk of radiation-induced damage in CC vs. CG/GG model (OR = 1.86, 95 % CI = 1.21-2.83) among mixed ethnicities which are mainly comprised of white people. When the data was stratified by organ-damaged, a significant association was only observed in the esophagus group (OR = 2.94, 95 % CI = 1.35-6.37, for CC vs. CG/GG model). In conclusion, the present study demonstrated that the rs2868371 genotypes of HSPB1 might be associated with radiation-induced esophagus damage risk, especially in Caucasians but not in the Asian population. PMID:26874728

  10. DIADDHEM set-up: New IBA facility for studying the helium behavior in nuclear glasses

    NASA Astrophysics Data System (ADS)

    Chamssedine, F.; Sauvage, T.; Peuget, S.

    2010-06-01

    The immobilization of fission products and minor actinides by vitrification is the reference process for industrial management of high-level radioactive wastes generated from spent fuel reprocessing. The glassy matrix is subjected to radiation damage and radiogenic helium generation due to the alpha decays of minor actinides. A specific experimental study has been conducted to better understand the behavior of helium and its diffusion mechanisms in the borosilicate glass. Helium production is simulated by external irradiation with 3He + ions at a concentration (2 × 10 15 He cm -2) equivalent to the one obtained after 1000 years of glass storage. He diffusion coefficients as function of temperature are extracted from the evolution of the depth profiles after annealing. The 3He(d, α) 1H Nuclear Reaction Analysis (NRA) technique is successfully used for in situ low-temperature measurements of depth profiles. Its high depth resolution allows detecting helium mobility at a temperature as low as 250 K and the presence of a trapped helium fraction. The good agreement of our first values of diffusion coefficients with the literature data highlights the relevance of the implantation technique in the study of helium diffusion mechanisms in borosilicate glasses.

  11. Molecular dynamics study of radiation damage and microstructure evolution of zigzag single-walled carbon nanotubes under carbon ion incidence

    NASA Astrophysics Data System (ADS)

    Li, Huan; Tang, Xiaobin; Chen, Feida; Huang, Hai; Liu, Jian; Chen, Da

    2016-07-01

    The radiation damage and microstructure evolution of different zigzag single-walled carbon nanotubes (SWCNTs) were investigated under incident carbon ion by molecular dynamics (MD) simulations. The radiation damage of SWCNTs under incident carbon ion with energy ranging from 25 eV to 1 keV at 300 K showed many differences at different incident sites, and the defect production increased to the maximum value with the increase in incident ion energy, and slightly decreased but stayed fairly stable within the majority of the energy range. The maximum damage of SWCNTs appeared when the incident ion energy reached 200 eV and the level of damage was directly proportional to incident ion fluence. The radiation damage was also studied at 100 K and 700 K and the defect production decreased distinctly with rising temperature because radiation-induced defects would anneal and recombine by saturating dangling bonds and reconstructing carbon network at the higher temperature. Furthermore, the stability of a large-diameter tube surpassed that of a thin one under the same radiation environments.

  12. Fundamental Processes of Coupled Radiation Damage and Mechanical Behavior in Nuclear Fuel Materials for High Temperature Reactors

    SciTech Connect

    Phillpot, Simon; Tulenko, James

    2011-09-08

    The objective of this work has been to elucidate the relationship among microstructure, radiation damage and mechanical properties for nuclear fuel materials. As representative nuclear materials, we have taken an hcp metal (Mg as a generic metal, and Ti alloys for fast reactors) and UO2 (representing fuel). The degradation of the thermo-mechanical behavior of nuclear fuels under irradiation, both the fissionable material itself and its cladding, is a longstanding issue of critical importance to the nuclear industry. There are experimental indications that nanocrystalline metals and ceramics may be more resistant to radiation damage than their coarse-grained counterparts. The objective of this project look at the effect of microstructure on radiation damage and mechanical behavior in these materials. The approach to be taken was state-of-the-art, large-scale atomic-level simulation. This systematic simulation program of the effects of irradiation on the structure and mechanical properties of polycrystalline Ti and UO2 identified radiation damage mechanisms. Moreover, it will provided important insights into behavior that can be expected in nanocrystalline microstructures and, by extension, nanocomposites. The fundamental insights from this work can be expected to help in the design microstructures that are less susceptible to radiation damage and thermomechanical degradation.

  13. Mechanism of Radiation Damage Reduction in Equiatomic Multicomponent Single Phase Alloys.

    PubMed

    Granberg, F; Nordlund, K; Ullah, Mohammad W; Jin, K; Lu, C; Bei, H; Wang, L M; Djurabekova, F; Weber, W J; Zhang, Y

    2016-04-01

    Recently a new class of metal alloys, of single-phase multicomponent composition at roughly equal atomic concentrations ("equiatomic"), have been shown to exhibit promising mechanical, magnetic, and corrosion resistance properties, in particular, at high temperatures. These features make them potential candidates for components of next-generation nuclear reactors and other high-radiation environments that will involve high temperatures combined with corrosive environments and extreme radiation exposure. In spite of a wide range of recent studies of many important properties of these alloys, their radiation tolerance at high doses remains unexplored. In this work, a combination of experimental and modeling efforts reveals a substantial reduction of damage accumulation under prolonged irradiation in single-phase NiFe and NiCoCr alloys compared to elemental Ni. This effect is explained by reduced dislocation mobility, which leads to slower growth of large dislocation structures. Moreover, there is no observable phase separation, ordering, or amorphization, pointing to a high phase stability of this class of alloys. PMID:27081990

  14. Mechanism of Radiation Damage Reduction in Equiatomic Multicomponent Single Phase Alloys

    NASA Astrophysics Data System (ADS)

    Granberg, F.; Nordlund, K.; Ullah, Mohammad W.; Jin, K.; Lu, C.; Bei, H.; Wang, L. M.; Djurabekova, F.; Weber, W. J.; Zhang, Y.

    2016-04-01

    Recently a new class of metal alloys, of single-phase multicomponent composition at roughly equal atomic concentrations ("equiatomic"), have been shown to exhibit promising mechanical, magnetic, and corrosion resistance properties, in particular, at high temperatures. These features make them potential candidates for components of next-generation nuclear reactors and other high-radiation environments that will involve high temperatures combined with corrosive environments and extreme radiation exposure. In spite of a wide range of recent studies of many important properties of these alloys, their radiation tolerance at high doses remains unexplored. In this work, a combination of experimental and modeling efforts reveals a substantial reduction of damage accumulation under prolonged irradiation in single-phase NiFe and NiCoCr alloys compared to elemental Ni. This effect is explained by reduced dislocation mobility, which leads to slower growth of large dislocation structures. Moreover, there is no observable phase separation, ordering, or amorphization, pointing to a high phase stability of this class of alloys.

  15. Anisotropic mechanical properties of zircon and the effect of radiation damage

    DOE PAGESBeta

    Beirau, Tobias; Nix, William D.; Bismayer, Ulrich; Boatner, Lynn A.; Isaacson, Scott G.; Ewing, Rodney C.

    2016-06-02

    Our study provides new insights into the relationship between radiation-dose-dependent structural damage, due to natural U and Th impurities, and the anisotropic mechanical properties (Poisson s ratio, elastic modulus and hardness) of zircon. Natural zircon samples from Sri Lanka (see Muarakami et al. 1991) and synthetic samples, covering a dose range of zero up to 6.8 x 1018 -decays/g, have been studied by nanoindentation. Measurements along the [100] crystallographic direction and calculations, based on elastic stiffness constants determined by zkan (1976), revealed a general radiation-induced decrease in stiffness (~ 54 %) and hardness (~ 48 %) and an increase ofmore » the Poisson s ratio (~ 54 %) with increasing dose. Additional indentations on selected samples along the [001] allowed one to follow the amorphization process to the point that the mechanical properties are isotropic. This work shows that the radiation-dose-dependent changes of the mechanical properties of zircon can be directly correlated with the amorphous fraction as determined by previous investigations with local and global probes (Rios et al. 2000a; Farnan and Salje 2001; Zhang and Salje 2001). This agreement, revealed by the different methods, indicates a huge influence of structural and even local phenomena on the macroscopic mechanical properties.« less

  16. Radiation damage studies on STAR250 CMOS sensor at 300 keV for electron microscopy

    NASA Astrophysics Data System (ADS)

    Faruqi, A. R.; Henderson, R.; Holmes, J.

    2006-09-01

    There is a pressing need for better electronic detectors to replace film for recording high-resolution images using electron cryomicroscopy. Our previous work has shown that direct electron detection in CMOS sensors is promising in terms of resolution and efficiency at 120 keV [A.R. Faruqi, R. Henderson, M. Prydderch, R. Turchetta, P. Allport, A. Evans, Nucl. Instr. and Meth. 546 (2005) 170], but in addition, the detectors must not be damaged by the electron irradiation. We now present new measurements on the radiation tolerance of a 25 μm pitch CMOS active-pixel sensor, the STAR250, which was designed by FillFactory using radiation-hard technology for space applications. Our tests on the STAR250 aimed to establish the imaging performance at 300 keV following irradiation. The residual contrast, measured on shadow images of a 300 mesh grid, was >80% after corrections for increased dark current, following irradiation with up to 5×10 7 electrons/pixel (equivalent to 80,000 electron/μm 2). A CMOS sensor with this degree of radiation tolerance would survive a year of normal usage for low-dose electron cryomicroscopy, which is a very useful advance.

  17. Biophysical modelling of early and delayed radiation damage at chromosome level

    NASA Astrophysics Data System (ADS)

    Andreev, S.; Eidelman, Y.

    Exposure by ionising radiation increases cancer risk in human population Cancer is thought to originate from an altered expression of certain number of specific genes It is now widely recognised that chromosome aberrations CA are involved in stable change in expression of genes by gain or loss of their functions Thus CA can contribute to initiation or progression of cancer Therefore understanding mechanisms of CA formation in the course of cancer development might be valuable tool for quantification and prognosis of different stages of radiation carcinogenesis Early CA are defined as aberrations induced in first post-irradiation mitotic cycle The present work describes the original biophysical technique for early CA modelling It includes the following simulation steps the ionising particle track structure the structural organisation of all chromosomes in G 0 G 1 cell nucleus spatial distribution of radiation induced DNA double-strand breaks dsb within chromosomes dsb rejoining and misrejoining modelling cell cycle taking into account mitotic delay which results in complex time dependence of aberrant cells in first mitosis The results on prediction of dose-response curves for simple and complex CA measured in cells undergoing first division cycle are presented in comparison with recent experimental data There is increasing evidence that CA are also observed in descendents of irradiated cells many generations after direct DNA damage These delayed CA or chromosome instability CI are thought to be a manifestation of genome

  18. Radiation damage to Kokchetav UHPM diamonds in zircon: Variations in Raman, photoluminescence, and cathodoluminescence spectra

    NASA Astrophysics Data System (ADS)

    Shimizu, Rentaro; Ogasawara, Yoshihide

    2014-10-01

    We conducted detailed in-situ Raman, photoluminescence (PL) and cathodoluminescence (CL) studies on microdiamonds in a tourmaline-rich quartzofeldspathic rock from the Kokchetav Massif, Kazakhstan. The microdiamonds occur as inclusions in the cores of K-tourmaline and in zoned zircons with varying U contents. The results of 2D Raman mapping of zircon showed that the U-rich parts were more metamictized than the U-poor parts. All the diamonds showed a strong Raman band at approximately 1332 cm- 1, however, the features of the Raman bands were distinctly different depending on the host minerals. On the one hand, diamonds in tourmaline had a sharp Raman band that is similar to that of kimberlite diamonds [full width at half maximum (FWHM): 2-3 cm- 1]. On the other hand, diamonds in zircon had a broad and downshifted band compared to those in tourmaline. In particular, diamonds in U-rich cores of zircons (up to 0.15 wt.% UO2) showed broader and more downshifted Raman bands (FWHMs and peak positions varied up to 9.3 cm- 1 and 1328 cm- 1, respectively), with additional small bands at approximately 1490 cm- 1 and 1630 cm- 1. A negative correlation was observed between the peak position and the FWHM of the principal Raman band of microdiamonds. Furthermore, the PL and CL spectra showed systematic variations. Diamonds in zircons with low to moderate U-concentrations had very strong PL and CL compared to diamonds in U-rich zircons and in tourmalines. Several characteristic peaks appeared in the PL and CL spectra, indicating the presence of irradiation and/or nitrogen-related point defects in the diamonds. PL and CL of microdiamonds in high-U zircon were weak, but still showed irradiation-related peaks. The relationship between the occurrence of microdiamonds (i.e., corresponding to the estimated total α-dose since crystallization) and the Raman, PL, and CL spectral characteristics of microdiamonds strongly suggests that radiation damage predominantly caused by

  19. REC-2006-A Fractionated Extract of Podophyllum hexandrum Protects Cellular DNA from Radiation-Induced Damage by Reducing the Initial Damage and Enhancing Its Repair In Vivo.

    PubMed

    Chaudhary, Pankaj; Shukla, Sandeep Kumar; Sharma, Rakesh Kumar

    2011-01-01

    Podophyllum hexandrum, a perennial herb commonly known as the Himalayan May Apple, is well known in Indian and Chinese traditional systems of medicine. P. hexandrum has been widely used for the treatment of venereal warts, skin infections, bacterial and viral infections, and different cancers of the brain, lung and bladder. This study aimed at elucidating the effect of REC-2006, a bioactive fractionated extract from the rhizome of P. hexandrum, on the kinetics of induction and repair of radiation-induced DNA damage in murine thymocytes in vivo. We evaluated its effect on non-specific radiation-induced DNA damage by the alkaline halo assay in terms of relative nuclear spreading factor (RNSF) and gene-specific radiation-induced DNA damage via semi-quantitative polymerase chain reaction. Whole body exposure of animals with gamma rays (10 Gy) caused a significant amount of DNA damage in thymocytes (RNSF values 17.7 ± 0.47, 12.96 ± 1.64 and 3.3 ± 0.014) and a reduction in the amplification of β-globin gene to 0, 28 and 43% at 0, 15 and 60 min, respectively. Administrating REC-2006 at a radioprotective concentration (15 mg kg(-1) body weight) 1 h before irradiation resulted in time-dependent reduction of DNA damage evident as a decrease in RNSF values 6.156 ± 0.576, 1.647 ± 0.534 and 0.496 ± 0.012, and an increase in β-globin gene amplification 36, 95 and 99%, at 0, 15 and 60 min, respectively. REC-2006 scavenged radiation-induced hydroxyl radicals in a dose-dependent manner stabilized DPPH free radicals and also inhibited superoxide anions. Various polyphenols and flavonoides present in REC-2006 might contribute to scavenging of radiation-induced free radicals, thereby preventing DNA damage and stimulating its repair. PMID:20008078

  20. Global radiation damage at 300 and 260 K with dose rates approaching 1 MGy s[superscript -1

    SciTech Connect

    Warkentin, Matthew; Badeau, Ryan; Hopkins, Jesse B.; Mulichak, Anne M.; Keefe, Lisa J.; Thorne, Robert E.

    2012-02-27

    Global radiation damage to 19 thaumatin crystals has been measured using dose rates from 3 to 680 kGy s{sup -1}. At room temperature damage per unit dose appears to be roughly independent of dose rate, suggesting that the timescales for important damage processes are less than {approx}1 s. However, at T = 260 K approximately half of the global damage manifested at dose rates of {approx}10 kGy s{sup -1} can be outrun by collecting data at 680 kGy s{sup -1}. Appreciable sample-to-sample variability in global radiation sensitivity at fixed dose rate is observed. This variability cannot be accounted for by errors in dose calculation, crystal slippage or the size of the data sets in the assay.

  1. Joint modeling of thermal creep and radiation damage interaction with gas permeability and release dynamics: The role of percolation

    NASA Astrophysics Data System (ADS)

    Ovaska, M.; Alava, M. J.

    2015-10-01

    Nuclear fuel material is an example of a sintered, porous ceramic material. We formulate a two-dimensional model which couples three physical mechanisms in the material: (scalar) damage accumulation by thermal creep and radiation effects, porosity changes due to the damage, and the time-dependent diffusion of (radiation-induced) gases in the pore system thus created. The most important effect in the dynamics arises from the process where the pore system is swept through the percolation transition. The main conclusions that can be drawn concern the fractional gas release and its dependence on the three effects present in the damage dynamics: creep, radiation-induced bubble formation, and recovery due to bubble closure. In the main, the model reproduces the experimentally observed quick gas release phenomenon qualitatively.

  2. Frozen human cells can record radiation damage accumulated during space flight: mutation induction and radioadaptation.

    PubMed

    Yatagai, Fumio; Honma, Masamitsu; Takahashi, Akihisa; Omori, Katsunori; Suzuki, Hiromi; Shimazu, Toru; Seki, Masaya; Hashizume, Toko; Ukai, Akiko; Sugasawa, Kaoru; Abe, Tomoko; Dohmae, Naoshi; Enomoto, Shuichi; Ohnishi, Takeo; Gordon, Alasdair; Ishioka, Noriaki

    2011-03-01

    To estimate the space-radiation effects separately from other space-environmental effects such as microgravity, frozen human lymphoblastoid TK6 cells were sent to the "Kibo" module of the International Space Station (ISS), preserved under frozen condition during the mission and finally recovered to Earth (after a total of 134 days flight, 72 mSv). Biological assays were performed on the cells recovered to Earth. We observed a tendency of increase (2.3-fold) in thymidine kinase deficient (TK(-)) mutations over the ground control. Loss of heterozygosity (LOH) analysis on the mutants also demonstrated a tendency of increase in proportion of the large deletion (beyond the TK locus) events, 6/41 in the in-flight samples and 1/17 in the ground control. Furthermore, in-flight samples exhibited 48% of the ground-control level in TK(-) mutation frequency upon exposure to a subsequent 2 Gy dose of X-rays, suggesting a tendency of radioadaptation when compared with the ground-control samples. The tendency of radioadaptation was also supported by the post-flight assays on DNA double-strand break repair: a 1.8- and 1.7-fold higher efficiency of in-flight samples compared to ground control via non-homologous end-joining and homologous recombination, respectively. These observations suggest that this system can be used as a biodosimeter, because DNA damage generated by space radiation is considered to be accumulated in the cells preserved frozen during the mission, Furthermore, this system is also suggested to be applicable for evaluating various cellular responses to low-dose space radiation, providing a better understanding of biological space-radiation effects as well as estimation of health influences of future space explores. PMID:21161544

  3. Cell to Cell Variability of Radiation-Induced Foci: Relation between Observed Damage and Energy Deposition.

    PubMed

    Gruel, Gaëtan; Villagrasa, Carmen; Voisin, Pascale; Clairand, Isabelle; Benderitter, Marc; Bottollier-Depois, Jean-François; Barquinero, Joan Francesc

    2016-01-01

    Most studies that aim to understand the interactions between different types of photon radiation and cellular DNA assume homogeneous cell irradiation, with all cells receiving the same amount of energy. The level of DNA damage is therefore generally determined by averaging it over the entire population of exposed cells. However, evaluating the molecular consequences of a stochastic phenomenon such as energy deposition of ionizing radiation by measuring only an average effect may not be sufficient for understanding some aspects of the cellular response to this radiation. The variance among the cells associated with this average effect may also be important for the behaviour of irradiated tissue. In this study, we accurately estimated the distribution of the number of radiation-induced γH2AX foci (RIF) per cell nucleus in a large population of endothelial cells exposed to 3 macroscopic doses of gamma rays from 60Co. The number of RIF varied significantly and reproducibly from cell to cell, with its relative standard deviation ranging from 36% to 18% depending on the macroscopic dose delivered. Interestingly, this relative cell-to-cell variability increased as the dose decreased, contrary to the mean RIF count per cell. This result shows that the dose effect, in terms of the number of DNA lesions indicated by RIF is not as simple as a purely proportional relation in which relative SD is constant with dose. To analyse the origins of this observed variability, we calculated the spread of the specific energy distribution for the different target volumes and subvolumes in which RIF can be generated. Variances, standard deviations and relative standard deviations all changed similarly from dose to dose for biological and calculated microdosimetric values. This similarity is an important argument that supports the hypothesis of the conservation of the association between the number of RIF per nucleus and the specific energy per DNA molecule. This comparison allowed us to

  4. Cell to Cell Variability of Radiation-Induced Foci: Relation between Observed Damage and Energy Deposition

    PubMed Central

    Voisin, Pascale; Clairand, Isabelle; Benderitter, Marc; Bottollier-Depois, Jean-François; Barquinero, Joan Francesc

    2016-01-01

    Most studies that aim to understand the interactions between different types of photon radiation and cellular DNA assume homogeneous cell irradiation, with all cells receiving the same amount of energy. The level of DNA damage is therefore generally determined by averaging it over the entire population of exposed cells. However, evaluating the molecular consequences of a stochastic phenomenon such as energy deposition of ionizing radiation by measuring only an average effect may not be sufficient for understanding some aspects of the cellular response to this radiation. The variance among the cells associated with this average effect may also be important for the behaviour of irradiated tissue. In this study, we accurately estimated the distribution of the number of radiation-induced γH2AX foci (RIF) per cell nucleus in a large population of endothelial cells exposed to 3 macroscopic doses of gamma rays from 60Co. The number of RIF varied significantly and reproducibly from cell to cell, with its relative standard deviation ranging from 36% to 18% depending on the macroscopic dose delivered. Interestingly, this relative cell-to-cell variability increased as the dose decreased, contrary to the mean RIF count per cell. This result shows that the dose effect, in terms of the number of DNA lesions indicated by RIF is not as simple as a purely proportional relation in which relative SD is constant with dose. To analyse the origins of this observed variability, we calculated the spread of the specific energy distribution for the different target volumes and subvolumes in which RIF can be generated. Variances, standard deviations and relative standard deviations all changed similarly from dose to dose for biological and calculated microdosimetric values. This similarity is an important argument that supports the hypothesis of the conservation of the association between the number of RIF per nucleus and the specific energy per DNA molecule. This comparison allowed us to

  5. A computational investigation on radiation damage and activation of structural material for C-ADS

    NASA Astrophysics Data System (ADS)

    Liang, Tairan; Shen, Fei; Yin, Wen; Yu, Quanzhi; Liang, Tianjiao

    2015-11-01

    The C-ADS (China Accelerator-Driven Subcritical System) project, which aims at transmuting high-level radiotoxic waste (HLW) and power generation, is now in the research and development stage. In this paper, a simplified ADS model is set up based on the IAEA Th-ADS benchmark calculation model, then the radiation damage as well as the residual radioactivity of the structural material are estimated using the Monte Carlo simulation method. The peak displacement production rate, gas productions, activity and residual dose rate of the structural components like beam window and outer casing of subcritical reactor core are calculated. The calculation methods and the corresponding results provide the basic reference for making reasonable predictions for the lifetime and maintenance operations of the structural material of C-ADS.

  6. Radiation Damage in Nuclear Fuel for Advanced Burner Reactors: Modeling and Experimental Validation

    SciTech Connect

    Jensen, Niels Gronbech; Asta, Mark; Ozolins, Nigel Browning'Vidvuds; de Walle, Axel van; Wolverton, Christopher

    2011-12-29

    The consortium has completed its existence and we are here highlighting work and accomplishments. As outlined in the proposal, the objective of the work was to advance the theoretical understanding of advanced nuclear fuel materials (oxides) toward a comprehensive modeling strategy that incorporates the different relevant scales involved in radiation damage in oxide fuels. Approaching this we set out to investigate and develop a set of directions: 1) Fission fragment and ion trajectory studies through advanced molecular dynamics methods that allow for statistical multi-scale simulations. This work also includes an investigation of appropriate interatomic force fields useful for the energetic multi-scale phenomena of high energy collisions; 2) Studies of defect and gas bubble formation through electronic structure and Monte Carlo simulations; and 3) an experimental component for the characterization of materials such that comparisons can be obtained between theory and experiment.

  7. Radiation damage mechanisms for luminescence in Eu-doped GaN

    SciTech Connect

    Tringe, J. W.; Felter, T. E.; Talley, C. E.; Morse, J. D.; Stevens, C. G.; Castelaz, J. M.; Wetzel, C.

    2007-03-01

    Thin films of Eu-doped GaN were irradiated with 500 keV He{sup +} ions to understand radiation damage mechanisms and to quantify luminescence efficiency. The dependence of ion-beam-induced luminescence intensity on ion fluence was consistent with the simultaneous creation of nonradiative defects and the destruction of luminescent centers associated with 4f-4f core-level transitions in Eu{sup 3+}. This model contrasts with a previous description which takes into account only nonradiative defect generation in GaN:Eu. Based on light from a BaF{sub 2} scintillator standard, the luminescent energy generation efficiency of GaN:Eu films doped to {approx}3x10{sup 18} cm{sup -3} Eu is estimated to be {approx}0.1%.

  8. Resistance of holograms made in Polaroid DMP128 photopolymer to ionizing radiation damage

    SciTech Connect

    Golden, J.P.; Summers, G.P.; Carter, W.H.

    1988-11-01

    Because of their light weight and general wave-front-transforming ability, holograms appear potentially useful as beam correctors and collimators for diode-laser arrays in intersatellite optical data links. However, to survive in space a hologram must withstand damage from electrons and protons trapped in the Van Allen belts. We have found that holograms made with Polaroid DMP128 photopolymer on Suprasil-2 can withstand 63-MeV protons up to a total dose of 2 Mrad in (Si) and withstand /sup 60/Co gamma rays up to a total dose of 2 Mrad in (Si) without loss of diffraction efficiency. It appears that these holograms are sufficiently radiation hard for space application.

  9. Study of radiation damage in ODS steels by positron annihilation spectroscopy

    NASA Astrophysics Data System (ADS)

    Bartošová, I.; Bouhaddane, A.; Dománková, M.; Slugeň, V.; Wall, D.; Selim, F. A.

    2016-01-01

    Microstructure of various oxide-dispersion-strengthened (ODS) steels with 15% chromium content was studied in term of vacancy defects presence and their accumulation after defined irradiation treatment, respectively. Studied materials originated from Kyoto University and studied via IAEA collaborative project. Samples were characterized “as received” by positron annihilation lifetime spectroscopy and their microstructure was examined by transmission electron microscopy as well. Samples were afterwards irradiated in Washington State University Nuclear Radiation Center via a strong gamma source (6TBq). Damage induced by gamma irradiation was evaluated by positron lifetime measurements in emphasis on defect accumulation in the materials. We have demonstrated strong defect production induced by gamma irradiation which results from positron measurement data.

  10. On radiation damage in FIB-prepared softwood samples measured by scanning X-ray diffraction.

    PubMed

    Storm, Selina; Ogurreck, Malte; Laipple, Daniel; Krywka, Christina; Burghammer, Manfred; Di Cola, Emanuela; Müller, Martin

    2015-03-01

    The high flux density encountered in scanning X-ray nanodiffraction experiments can lead to severe radiation damage to biological samples. However, this technique is a suitable tool for investigating samples to high spatial resolution. The layered cell wall structure of softwood tracheids is an interesting system which has been extensively studied using this method. The tracheid cell has a complex geometry, which requires the sample to be prepared by cutting it perpendicularly to the cell wall axis. Focused ion beam (FIB) milling in combination with scanning electron microscopy allows precise alignment and cutting without splintering. Here, results of a scanning X-ray diffraction experiment performed on a biological sample prepared with a focused ion beam of gallium atoms are reported for the first time. It is shown that samples prepared and measured in this way suffer from the incorporation of gallium atoms up to a surprisingly large depth of 1 µm. PMID:25723928

  11. Influence of XRCC1 Genetic Polymorphisms on Ionizing Radiation-Induced DNA Damage and Repair

    PubMed Central

    Sterpone, Silvia; Cozzi, Renata

    2010-01-01

    It is well known that ionizing radiation (IR) can damage DNA through a direct action, producing single- and double-strand breaks on DNA double helix, as well as an indirect effect by generating oxygen reactive species in the cells. Mammals have evolved several and distinct DNA repair pathways in order to maintain genomic stability and avoid tumour cell transformation. This review reports important data showing a huge interindividual variability on sensitivity to IR and in susceptibility to developing cancer; this variability is principally represented by genetic polymorphisms, that is, DNA repair gene polymorphisms. In particular we have focussed on single nucleotide polymorphisms (SNPs) of XRCC1, a gene that encodes for a scaffold protein involved basically in Base Excision Repair (BER). In this paper we have reported and presented recent studies that show an influence of XRCC1 variants on DNA repair capacity and susceptibility to breast cancer. PMID:20798883

  12. Microbeam Radiation-Induced Tissue Damage Depends on the Stage of Vascular Maturation

    SciTech Connect

    Sabatasso, Sara; Laissue, Jean Albert; Hlushchuk, Ruslan; Graber, Werner; Bravin, Alberto; Braeuer-Krisch, Elke; Corde, Stephanie; Blattmann, Hans; Gruber, Guenther; Djonov, Valentin

    2011-08-01

    Purpose: To explore the effects of microbeam radiation (MR) on vascular biology, we used the chick chorioallantoic membrane (CAM) model of an almost pure vascular system with immature vessels (lacking periendothelial coverage) at Day 8 and mature vessels (with coverage) at Day 12 of development. Methods and Materials: CAMs were irradiated with microplanar beams (width, {approx}25 {mu}m; interbeam spacing, {approx}200 {mu}m) at entrance doses of 200 or 300 Gy and, for comparison, with a broad beam (seamless radiation [SLR]), with entrance doses of 5 to 40 Gy. Results: In vivo monitoring of Day-8 CAM vasculature 6 h after 200 Gy MR revealed a near total destruction of the immature capillary plexus. Conversely, 200 Gy MR barely affected Day-12 CAM mature microvasculature. Morphological evaluation of Day-12 CAMs after the dose was increased to 300 Gy revealed opened interendothelial junctions, which could explain the transient mesenchymal edema immediately after irradiation. Electron micrographs revealed cytoplasmic vacuolization of endothelial cells in the beam path, with disrupted luminal surfaces; often the lumen was engorged with erythrocytes and leukocytes. After 30 min, the capillary plexus adopted a striated metronomic pattern, with alternating destroyed and intact zones, corresponding to the beam and the interbeam paths within the array. SLR at a dose of 10 Gy caused growth retardation, resulting in a remarkable reduction in the vascular endpoint density 24 h postirradiation. A dose of 40 Gy damaged the entire CAM vasculature. Conclusions: The effects of MR are mediated by capillary damage, with tissue injury caused by insufficient blood supply. Vascular toxicity and physiological effects of MR depend on the stage of capillary maturation and appear in the first 15 to 60 min after irradiation. Conversely, the effects of SLR, due to the arrest of cell proliferation, persist for a longer time.

  13. Atomistic investigation of Cr influence on primary radiation damage in Fe-12 at.% Cr grain boundaries

    NASA Astrophysics Data System (ADS)

    Esfandiarpour, A.; Feghhi, S. A. H.; Arjhangmehr, A.

    2016-08-01

    In this paper, we investigate the influence of Cr on the primary radiation damage in Fe-12 at.% Cr with different atomic grain boundaries (GBs). Four different GB structures, two twists and two symmetric tilt boundaries are selected as the model structures. The primary radiation damage near each GB in α-Fe and Fe-12 at.% Cr is simulated using Molecular Dynamics for 9 keV primary knock-on atoms with velocity vectors perpendicular to the GB plane. In agreement with previous works, the results indicate that the atomic GBs are biased toward interstitials and due to the reduction of ‘in-cascade’ interstitial-vacancy annihilation rates, vacancies accumulate in the bulk grains. The minimum defect production occurs when the overlap between cascade center and GB plane is maximum; in contrast, the number of residual defects in the bulk (vacancies and interstitials) increases when the overlap decreases. Moreover, we find that the presence of Cr hardly affects the number of residual defects in the grain interiors, and causes a Cr-enrichment in the surviving self-interstitial atoms in bulk during relaxation of the primary cascades—also in agreement with previous studies. Further, in order to study the effect of 12 at.% Cr on the energetic and kinetic properties of vacancies near the atomic GBs, we calculate formation energies and diffusion barriers of defects using Molecular Static and climbing-Nudged Elastic Band methods. The results reveal that the vacancies energetically and kinetically tend to form and cluster around the GB plane due to the substantial reduction of their formation energies and migration barriers in layers close to the GB center and are immobile on the simulated time frame (~ps).

  14. Preparation and characterization of {sup 238}Pu-ceramics for radiation damage experiments

    SciTech Connect

    DM Strachan; RD Scheele; WC Buchmiller; JD Vienna; RL Sell; RJ Elovich

    2000-06-15

    As a result of treaty agreements between Russia and the US, portions of their respective plutonium and nuclear weapons stockpiles have been declared excess. In support of the US Department of Energy's 1998 decision to pursue immobilization of a portion of the remaining Pu in a titanate-based ceramic, the authors prepared nearly 200 radiation-damage test specimens of five Pu- and {sup 238}Pu-ceramics containing 10 mass% Pu to determine the effects of irradiation from the contained Pu and U on the ceramic. The five Pu-ceramics were (1) phase-pure pyrochlore [ideally, Ca(U, Pu)Ti{sub 2}O{sub 7}], (2) pyrochlore-rich baseline, (3) pyrochlore-rich baseline with impurities, (4) phase-pure zirconolite [ideally Ca(U, Pu)Ti{sub 2}O{sub 7}], and (5) a zirconolite-rich baseline. These ceramics were prepared with either normal weapons-grade Pu, which is predominantly {sup 239}Pu, or {sup 238}Pu. The {sup 238}Pu accelerates the radiation damage relative to the {sup 239}Pu because of its much higher specific activity. The authors were unsuccessful in preparing phase-pure (Pu, U) brannerite, which is the third crystalline phase present in the baseline immobilization form. Since these materials will contain {approximately}10 mass% Pu and about 20 mass% U, radiation damage to the crystalline structure of these materials will occur overtime. As the material becomes damaged from the decay of the Pu and U, it is possible for the material to swell as both the alpha particles and recoiling atoms rupture chemical bonds within the solid. As the material changes density, cracking, perhaps in the form of microcracks, may occur. If cracking occurs in ceramic that has been placed in a repository, the calculated rate of radionuclide release if the can has corroded would increase proportionately to the increase in surface area. To investigate the effects of radiation damage on the five ceramics prepared, the authors are storing the specimens at 20, 125, and 250 C until the {sup 238}Pu specimens

  15. Protection against radiation-induced DNA damage by amino acids: a DFT study.

    PubMed

    Jena, N R; Mishra, P C; Suhai, S

    2009-04-23

    Direct and indirect radiation-induced DNA damage is associated with the formation of radical cations (G(+)) and radical anions (G(-)) of guanine, respectively. Deprotonation of G(+) and dehydrogenation of G(-) generate guanine neutral radical [G(-H)] and guanine anion [G(-H)(-)], respectively. These products are of worrisome concern, as they are involved in reactions that are related to certain lethal diseases. It has been observed that guanyl radicals can be repaired by amino acids having strong reducing properties that are believed to be the residues of DNA-bound proteins such as histones. As a result, repair of G(-H) and G(-H)(-) by the amino acids cysteine and tyrosine has been studied here in detail by density functional theory in both the gas phase and aqueous medium using the polarized continuum and Onsager solvation models of self-consistent reaction field theory. Solvation in aqueous medium using three explicit water molecules was also studied. Four equivalent tautomers of each the above radical and anion that will be formed through proton and hydrogen loss from all of the nitrogen centers of guanine radical cation and guanine radical anion, respectively, were considered in the present study. It was found that in both the gas phase and aqueous medium, normal guanine can be retrieved from its radical-damaged form by a hydrogen-atom-transfer (HT) mechanism. Normal guanine can also be retrieved from its anionic damaged form in both the gas phase and aqueous medium through a two-electron-coupled proton-transfer (TECPT) mechanism or a one-step hydrogen-atom- and electron-transfer (OSHET) mechanism. The present results are discussed in light of the experimental findings. PMID:19334703

  16. Ozone depletion and UVB radiation: Impact on plant DNA damage in southern South America

    PubMed Central

    Rousseaux, M. Cecilia; Ballaré, Carlos L.; Giordano, Carla V.; Scopel, Ana L.; Zima, Ana M.; Szwarcberg-Bracchitta, Mariela; Searles, Peter S.; Caldwell, Martyn M.; Díaz, Susana B.

    1999-01-01

    The primary motivation behind the considerable effort in studying stratospheric ozone depletion is the potential for biological consequences of increased solar UVB (280–315 nm) radiation. Yet, direct links between ozone depletion and biological impacts have been established only for organisms of Antarctic waters under the influence of the ozone “hole;” no direct evidence exists that ozone-related variations in UVB affect ecosystems of temperate latitudes. Indeed, calculations based on laboratory studies with plants suggest that the biological impact of ozone depletion (measured by the formation of cyclobutane pyrimidine dimers in DNA) is likely to be less marked than previously thought, because UVA quanta (315–400 nm) may also cause significant damage, and UVA is unaffected by ozone depletion. Herein, we show that the temperate ecosystems of southern South America have been subjected to increasingly high levels of ozone depletion during the last decade. We found that in the spring of 1997, despite frequent cloud cover, the passages of the ozone hole over Tierra del Fuego (55° S) caused concomitant increases in solar UV and that the enhanced ground-level UV led to significant increases in DNA damage in the native plant Gunnera magellanica. The fluctuations in solar UV explained a large proportion of the variation in DNA damage (up to 68%), particularly when the solar UV was weighted for biological effectiveness according to action spectra that assume a sharp decline in quantum efficiency with increasing wavelength from the UVB into the UVA regions of the spectrum. PMID:10611381

  17. The Effect of a Grape Seed Extract on Radiation-Induced DNA Damage in Human Lymphocytes

    NASA Astrophysics Data System (ADS)

    Dicu, Tiberius; Postescu, Ion D.; Foriş, Vasile; Brie, Ioana; Fischer-Fodor, Eva; Cernea, Valentin; Moldovan, Mircea; Cosma, Constantin

    2009-05-01

    Plant-derived antioxidants due to their phenolic compounds content are reported as potential candidates for reducing the levels of oxidative stress in living organisms. Grape seed extracts are very potent antioxidants and exhibit numerous interesting pharmacologic activities. Hydroethanolic (50/50, v/v) standardized extract was obtained from red grape seed (Vitis vinifera, variety Burgund Mare—BM). The total polyphenols content was evaluated by Folin-Ciocalteu procedure and expressed as μEq Gallic Acid/ml. The aim of this study was to evaluate the potential antioxidant effects of different concentrations of BM extract against 60Co γ-rays induced DNA damage in human lymphocytes. Samples of human lymphocytes were incubated with BM extract (12.5, 25.0 and 37.5 μEq GA/ml, respectively) administered at 30 minutes before in vitro irradiation with γ-rays (2 Gy). The DNA damage and repair in lymphocytes were evaluated using alkaline comet assay. Using the lesion score, the radiation-induced DNA damage was found to be significantly different (p<0.05) from control, both in the absence and presence of BM extract (except the lymphocytes treated with 37.5 μEq GA/ml BM extract). DNA repair analyzed by incubating the irradiated cells at 37° C and 5% CO2 atmosphere for 2 h, indicated a significant difference (p<0.05) in the lymphocytes group treated with 25.0 μEq GA/ml BM extract, immediately and two hours after irradiation. These results suggest radioprotective effects after treatment with BM extract in human lymphocytes.

  18. On the validity of empirical potentials for simulating radiation damage in graphite: a benchmark

    NASA Astrophysics Data System (ADS)

    Latham, C. D.; McKenna, A. J.; Trevethan, T. P.; Heggie, M. I.; Rayson, M. J.; Briddon, P. R.

    2015-08-01

    In this work, the ability of methods based on empirical potentials to simulate the effects of radiation damage in graphite is examined by comparing results for point defects, found using ab initio calculations based on density functional theory (DFT), with those given by two state of the art potentials: the Environment-Dependent Interatomic Potential (EDIP) and the Adaptive Intermolecular Reactive Empirical Bond Order potential (AIREBO). Formation energies for the interstitial, the vacancy and the Stone-Wales (5775) defect are all reasonably close to DFT values. Both EDIP and AIREBO can thus be suitable for the prompt defects in a cascade, for example. Both potentials suffer from arefacts. One is the pinch defect, where two α-atoms adopt a fourfold-coordinated sp3 configuration, that forms a cross-link between neighbouring graphene sheets. Another, for AIREBO only, is that its ground state vacancy structure is close to the transition state found by DFT for migration. The EDIP fails to reproduce the ground state self-interstitial structure given by DFT, but has nearly the same formation energy. Also, for both potentials, the energy barriers that control diffusion and the evolution of a damage cascade, are not well reproduced. In particular the EDIP gives a barrier to removal of the Stone-Wales defect as 0.9 eV against DFT's 4.5 eV. The suite of defect structures used is provided as supplementary information as a benchmark set for future potentials.

  19. Radiation-induced oxidative damage to the DNA-binding domain of the lactose repressor.

    PubMed

    Gillard, Nathalie; Goffinont, Stephane; Buré, Corinne; Davidkova, Marie; Maurizot, Jean-Claude; Cadene, Martine; Spotheim-Maurizot, Melanie

    2007-05-01

    Understanding the cellular effects of radiation-induced oxidation requires the unravelling of key molecular events, particularly damage to proteins with important cellular functions. The Escherichia coli lactose operon is a classical model of gene regulation systems. Its functional mechanism involves the specific binding of a protein, the repressor, to a specific DNA sequence, the operator. We have shown previously that upon irradiation with gamma-rays in solution, the repressor loses its ability to bind the operator. Water radiolysis generates hydroxyl radicals (OH* radicals) which attack the protein. Damage of the repressor DNA-binding domain, called the headpiece, is most likely to be responsible of this loss of function. Using CD, fluorescence spectroscopy and a combination of proteolytic cleavage with MS, we have examined the state of the irradiated headpiece. CD measurements revealed a dose-dependent conformational change involving metastable intermediate states. Fluorescence measurements showed a gradual degradation of tyrosine residues. MS was used to count the number of oxidations in different regions of the headpiece and to narrow down the parts of the sequence bearing oxidized residues. By calculating the relative probabilities of reaction of each amino acid with OH. radicals, we can predict the most probable oxidation targets. By comparing the experimental results with the predictions we conclude that Tyr7, Tyr12, Tyr17, Met42 and Tyr47 are the most likely hotspots of oxidation. The loss of repressor function is thus correlated with chemical modifications and conformational changes of the headpiece. PMID:17263689

  20. Measurement of radiation damage of water-based liquid scintillator and liquid scintillator

    DOE PAGESBeta

    Bignell, L. J.; Diwan, M. V.; Hans, S.; Jaffe, D. E.; Rosero, R.; Vigdor, S.; Viren, B.; Worcester, E.; Yeh, M.; Zhang, C.

    2015-10-19

    Liquid scintillating phantoms have been proposed as a means to perform real-time 3D dosimetry for proton therapy treatment plan verification. We have studied what effect radiation damage to the scintillator will have upon this application. We have performed measurements of the degradation of the light yield and optical attenuation length of liquid scintillator and water-based liquid scintillator after irradiation by 201 MeV proton beams that deposited doses of approximately 52 Gy, 300 Gy, and 800 Gy in the scintillator. Liquid scintillator and water-based liquid scintillator (composed of 5% scintillating phase) exhibit light yield reductions of 1.74 ± 0.55 % andmore » 1.31 ± 0.59 % after ≈ 800 Gy of proton dose, respectively. Some increased optical attenuation was observed in the irradiated samples, the measured reduction to the light yield is also due to damage to the scintillation light production. Based on our results and conservative estimates of the expected dose in a clinical context, a scintillating phantom used for proton therapy treatment plan verification would exhibit a systematic light yield reduction of approximately 0.1% after a year of operation.« less

  1. Radiation damage induced by MeV alpha particles in polycrystalline diamond films

    NASA Astrophysics Data System (ADS)

    Gonon, P.; Prawer, S.; Nugent, K. W.; Jamieson, D. N.

    1996-11-01

    Radiation damage induced by 2 MeV alpha particles in polycrystalline diamond films has been studied as a function of the irradiation dose D (1012≤D≤1017 cm-2). The films were characterized using Raman/photoluminescence spectroscopy and I(V,T) measurements. The results can be summarized as follows. In undoped samples the H3 luminescent center (N-V-N) is observed for D≥1014 cm-2. The Raman diamond peak is broadened and shifted to lower frequencies for D≳1015 cm-2. No new graphitic component is detected after irradiation. On the contrary graphitic sp2 defects are annealed by irradiation. For D=3×1016 cm-2 new Raman defect peaks are detected at 1496 and 1635 cm-1. I(V,T) characteristics remain unaffected for D≤1016 cm-2. An increase in the conductivity is observed for D=3×1016 cm-2. At this dose we observe an activation energy of 0.4 eV and thermally stimulated currents related to defect levels at about 0.3 eV. A boron-doped sample (100 Ω cm) has been irradiated at 1017 cm-2 for comparison. After irradiation the conductivity of this sample is reduced and the activation energy of the conductivity is also reduced. Less damage is detected by Raman spectroscopy in the B-doped material.

  2. Differential DNA damage signalling and apoptotic threshold correlate with mouse epiblast-specific hypersensitivity to radiation.

    PubMed

    Laurent, Audrey; Blasi, Francesco

    2015-11-01

    Between implantation and gastrulation, mouse pluripotent epiblast cells expand enormously in number and exhibit a remarkable hypersensitivity to DNA damage. Upon low-dose irradiation, they undergo mitotic arrest followed by p53-dependent apoptosis, whereas the other cell types simply arrest. This protective mechanism, active exclusively after E5.5 and lost during gastrulation, ensures the elimination of every mutated cell before its clonal expansion and is therefore expected to greatly increase fitness. We show that the insurgence of apoptosis relies on the epiblast-specific convergence of both increased DNA damage signalling and stronger pro-apoptotic balance. Although upstream Atm/Atr global activity and specific γH2AX phosphorylation are similar in all cell types of the embryo, 53BP1 recruitment at DNA breaks is immediately amplified only in epiblast cells after ionizing radiation. This correlates with rapid epiblast-specific activation of p53 and its transcriptional properties. Moreover, between E5.5 and E6.5 epiblast cells lower their apoptotic threshold by enhancing the expression of pro-apoptotic Bak and Bim and repressing the anti-apoptotic Bcl-xL. Thus, even after low-dose irradiation, the cytoplasmic priming of epiblast cells allows p53 to rapidly induce apoptosis via a partially transcription-independent mechanism. PMID:26395482

  3. Measurement of radiation damage of water-based liquid scintillator and liquid scintillator

    SciTech Connect

    Bignell, L. J.; Diwan, M. V.; Hans, S.; Jaffe, D. E.; Rosero, R.; Vigdor, S.; Viren, B.; Worcester, E.; Yeh, M.; Zhang, C.

    2015-10-19

    Liquid scintillating phantoms have been proposed as a means to perform real-time 3D dosimetry for proton therapy treatment plan verification. We have studied what effect radiation damage to the scintillator will have upon this application. We have performed measurements of the degradation of the light yield and optical attenuation length of liquid scintillator and water-based liquid scintillator after irradiation by 201 MeV proton beams that deposited doses of approximately 52 Gy, 300 Gy, and 800 Gy in the scintillator. Liquid scintillator and water-based liquid scintillator (composed of 5% scintillating phase) exhibit light yield reductions of 1.74 ± 0.55 % and 1.31 ± 0.59 % after ≈ 800 Gy of proton dose, respectively. Some increased optical attenuation was observed in the irradiated samples, the measured reduction to the light yield is also due to damage to the scintillation light production. Based on our results and conservative estimates of the expected dose in a clinical context, a scintillating phantom used for proton therapy treatment plan verification would exhibit a systematic light yield reduction of approximately 0.1% after a year of operation.

  4. On the validity of empirical potentials for simulating radiation damage in graphite: a benchmark.

    PubMed

    Latham, C D; McKenna, A J; Trevethan, T P; Heggie, M I; Rayson, M J; Briddon, P R

    2015-08-12

    In this work, the ability of methods based on empirical potentials to simulate the effects of radiation damage in graphite is examined by comparing results for point defects, found using ab initio calculations based on density functional theory (DFT), with those given by two state of the art potentials: the Environment-Dependent Interatomic Potential (EDIP) and the Adaptive Intermolecular Reactive Empirical Bond Order potential (AIREBO). Formation energies for the interstitial, the vacancy and the Stone-Wales (5775) defect are all reasonably close to DFT values. Both EDIP and AIREBO can thus be suitable for the prompt defects in a cascade, for example. Both potentials suffer from arefacts. One is the pinch defect, where two α-atoms adopt a fourfold-coordinated sp(3) configuration, that forms a cross-link between neighbouring graphene sheets. Another, for AIREBO only, is that its ground state vacancy structure is close to the transition state found by DFT for migration. The EDIP fails to reproduce the ground state self-interstitial structure given by DFT, but has nearly the same formation energy. Also, for both potentials, the energy barriers that control diffusion and the evolution of a damage cascade, are not well reproduced. In particular the EDIP gives a barrier to removal of the Stone-Wales defect as 0.9 eV against DFT's 4.5 eV. The suite of defect structures used is provided as supplementary information as a benchmark set for future potentials. PMID:26202454

  5. Resveratrol and its methoxy-derivatives as modulators of DNA damage induced by ionising radiation.

    PubMed

    Traversi, Gianandrea; Fiore, Mario; Leone, Stefano; Basso, Emiliano; Di Muzio, Elena; Polticelli, Fabio; Degrassi, Francesca; Cozzi, Renata

    2016-07-01

    Various naturally occurring stilbene-like compounds that are related to resveratrol (RSV) possess some of the beneficial effects of the parent molecule and provide even further benefits. Therefore, a series of methoxylated analogues of RSV were prepared with the aim of increasing antitumour and proapoptotic activity. In a previous article, we studied two methoxy-derivatives, pterostilbene (PTERO) and trimethoxystilbene (TRIMETHOXY), in which the first was formed by the substitution of two hydroxyl groups with two methoxy groups (trans-3,5-dimethoxy-4'-hydroxystilbene) and the second was formed by the replacement of all three OH groups with methoxy groups (trans-3,5,4'-trimethoxystilbene). Both methoxy-derivatives showed stronger antioxidant activity when compared with RSV. In the present article, we focused on the analysis of the ability of RSV and its two methoxylated derivatives to protect proliferating non-tumoural cells from the damage induced by ionising radiation (IR). First we showed that the methoxy derivatives, contrary to their parental compound, are unable to affect topoisomerase enzyme and consequently are not clastogenic per se Second we showed that both PTERO and TRIMETHOXY more efficiently reduce the chromosome damage induced by IR. Furthermore, TRIMETHOXY, but not PTERO, causes a delay in cell proliferation, particularly in mitosis progression increasing the number of cells in metaphase at the expense of prophases and ana/telophases. PMID:26819346

  6. Endothelial cells mitigate DNA damage and promote the regeneration of hematopoietic stem cells after radiation injury

    PubMed Central

    Zachman, Derek K.; Leon, Ronald P.; Das, Prerna; Goldman, Devorah C.; Hamlin, Kimberly L.; Guha, Chandan; Fleming, William H.

    2014-01-01

    Endothelial cells (ECs) are an essential component of the hematopoietic microenvironment, which maintains and regulates hematopoietic stem cells (HSCs). Although ECs can support the regeneration of otherwise lethally-irradiated HSCs, the mechanisms are not well understood. To further understand this phenomenon, we studied HSC regeneration from irradiated bone marrow using co-culture with human aortic endothelial cells (HAECs). Co-culture with HAECs induced a 24-fold expansion of long-term HSCs (CD150+, lineagelo, Sca-1+, c-Kit+; CD150+LSK cells) in vitro. These cells gave rise to functional hematopoietic stem and progenitor cells (HSPCs) with colony-forming activity, multilineage reconstitution and serial transplantation potential. Furthermore, HAECs significantly reduced DNA damage in irradiated LSK cells within 24 hours. Remarkably, we were able to delay the exposure of irradiated bone marrow to the regenerative, HAEC-derived signals for up to 48 hours and still rescue functional HSCs. G-CSF is the gold standard for promoting hematopoietic regeneration in vivo. However, when compared to HAECs, in vitro G-CSF treatment promoted lineage differentiation and regenerated 5-fold fewer CD150+LSK cells. Together, our results show that HAECs are powerful, direct mitigators of HSC injury and DNA damage. Identification of the HAEC-derived factors that rescue HSCs may lead to improved therapies for hematopoietic regeneration after radiation injury. PMID:23939266

  7. Regulation of homologous recombinational repair by lamin B1 in radiation-induced DNA damage.

    PubMed

    Liu, Ning-Ang; Sun, Jiying; Kono, Kazuteru; Horikoshi, Yasunori; Ikura, Tsuyoshi; Tong, Xing; Haraguchi, Tokuko; Tashiro, Satoshi

    2015-06-01

    DNA double-strand breaks (DSBs) are the major lethal lesion induced by ionizing radiation (IR). RAD51-dependent homologous recombination (HR) is one of the most important pathways in DSB repair and genome integrity maintenance. However, the mechanism of HR regulation by RAD51 remains unclear. To understand the mechanism of RAD51-dependent HR, we searched for interacting partners of RAD51 by a proteomics analysis and identified lamin B1 in human cells. Lamins are nuclear lamina proteins that play important roles in the structural organization of the nucleus and the regulation of chromosome functions. Immunoblotting analyses revealed that siRNA-mediated lamin B1 depletion repressed the DNA damage-dependent increase of RAD51 after IR. The repression was abolished by the proteasome inhibitor MG132, suggesting that lamin B1 stabilizes RAD51 by preventing proteasome-mediated degradation in cells with IR-induced DNA damage. We also showed that lamin B1 depletion repressed RAD51 focus formation and decreased the survival rates after IR. On the basis of these results, we propose that lamin B1 promotes DSB repair and cell survival by maintaining the RAD51 protein levels for HR upon DSB induction after IR. PMID:25733566

  8. Radiation-induced oxidative damage to the DNA-binding domain of the lactose repressor

    PubMed Central

    Gillard, Nathalie; Goffinont, Stephane; Buré, Corinne; Davidkova, Marie; Maurizot, Jean-Claude; Cadene, Martine; Spotheim-Maurizot, Melanie

    2007-01-01

    Understanding the cellular effects of radiation-induced oxidation requires the unravelling of key molecular events, particularly damage to proteins with important cellular functions. The Escherichia coli lactose operon is a classical model of gene regulation systems. Its functional mechanism involves the specific binding of a protein, the repressor, to a specific DNA sequence, the operator. We have shown previously that upon irradiation with γ-rays in solution, the repressor loses its ability to bind the operator. Water radiolysis generates hydroxyl radicals (OH· radicals) which attack the protein. Damage of the repressor DNA-binding domain, called the headpiece, is most likely to be responsible of this loss of function. Using CD, fluorescence spectroscopy and a combination of proteolytic cleavage with MS, we have examined the state of the irradiated headpiece. CD measurements revealed a dose-dependent conformational change involving metastable intermediate states. Fluorescence measurements showed a gradual degradation of tyrosine residues. MS was used to count the number of oxidations in different regions of the headpiece and to narrow down the parts of the sequence bearing oxidized residues. By calculating the relative probabilities of reaction of each amino acid with OH· radicals, we can predict the most probable oxidation targets. By comparing the experimental results with the predictions we conclude that Tyr7, Tyr12, Tyr17, Met42 and Tyr47 are the most likely hotspots of oxidation. The loss of repressor function is thus correlated with chemical modifications and conformational changes of the headpiece. PMID:17263689

  9. Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging

    DOE PAGESBeta

    Coughlan, H. D.; Darmanin, C.; Phillips, N. W.; Hofmann, F.; Clark, J. N.; Harder, R. J.; Vine, D. J.; Abbey, B.

    2015-04-29

    For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals where the weaker signal often necessitates the use of higher intensity beams to obtain the relevant data. Here, we employ a combination of techniques, including Bragg coherent diffractive imaging to characterise the radiation induced damage in a micron-sized protein crystal over time. The approach we adopt here could help screen for potential protein crystal candidates for measurement at X-ray free election laser sources.

  10. Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging

    PubMed Central

    Coughlan, H. D.; Darmanin, C.; Phillips, N. W.; Hofmann, F.; Clark, J. N.; Harder, R. J.; Vine, D. J.; Abbey, B.

    2015-01-01

    For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals where the weaker signal often necessitates the use of higher intensity beams to obtain the relevant data. Here, we employ a combination of techniques, including Bragg coherent diffractive imaging to characterise the radiation induced damage in a micron-sized protein crystal over time. The approach we adopt here could help screen for potential protein crystal candidates for measurement at X-ray free election laser sources. PMID:26798804

  11. Biomolecular Damage Induced by Ionizing Radiation: The Direct and Indirect Effects of Low-Energy Electrons on DNA

    NASA Astrophysics Data System (ADS)

    Alizadeh, Elahe; Orlando, Thomas M.; Sanche, Léon

    2015-04-01

    Many experimental and theoretical advances have recently allowed the study of direct and indirect effects of low-energy electrons (LEEs) on DNA damage. In an effort to explain how LEEs damage the human genome, researchers have focused efforts on LEE interactions with bacterial plasmids, DNA bases, sugar analogs, phosphate groups, and longer DNA moieties. Here, we summarize the current understanding of the fundamental mechanisms involved in LEE-induced damage of DNA and complex biomolecule films. Results obtained by several laboratories on films prepared and analyzed by different methods and irradiated with different electron-beam current densities and fluencies are presented. Despite varied conditions (e.g., film thicknesses and morphologies, intrinsic water content, substrate interactions, and extrinsic atmospheric compositions), comparisons show a striking resemblance in the types of damage produced and their yield functions. The potential of controlling this damage using molecular and nanoparticle targets with high LEE yields in targeted radiation-based cancer therapies is also discussed.

  12. Influence of radiation damage and isochronal annealing on the magnetic susceptibility of Pu(1-x)Am(x) alloys

    SciTech Connect

    McCall, S; Fluss, M; Chung, B; Haire, R

    2008-05-02

    Results of radiation damage in Pu and Pu{sub 1-x}Am{sub x} alloys studied with magnetic susceptibility, {chi}(T), and resistivity are presented. Damage accumulated at low temperatures increases {chi}(T) for all measured alloys, with the trend generally enhanced as the lattice expands. There is a trend towards saturation observable in the damage induced magnetic susceptibility data, that is not evident in similar damage induced resistivity data taken on the same specimen. A comparison of isochronal annealing curves measured by both resistivity and magnetic susceptibility on a 4.3at% Ga stabilized {delta}-Pu specimen show that Stage I annealing, where interstitials begin to move, is largely transparent to the magnetic measurement. This indicates that interstitials have little impact on the damage induced increase in the magnetic susceptibility. The isochronal annealing curves of the Pu{sub 1-x}Am{sub x} alloys do not show distinct annealing stages as expected for alloys. However, samples near 20% Am concentration show an unexpected increase in magnetization beginning when specimens are annealed to 35K. This behavior is also reflected in a time dependent increase in the magnetic susceptibility of damaged specimens indicative of first order kinetics. These results suggest there may be a metastable phase induced by radiation damage and annealing in Pu{sub 1-x}Am{sub x} alloys.

  13. Protection of radiation induced DNA and membrane damages by total triterpenes isolated from Ganoderma lucidum (Fr.) P. Karst.

    PubMed

    Smina, T P; Maurya, D K; Devasagayam, T P A; Janardhanan, K K

    2015-05-25

    The total triterpenes isolated from the fruiting bodies of Ganoderma lucidum was examined for its potential to prevent γ-radiation induced membrane damage in rat liver mitochondria and microsomes. The effects of total triterpenes on γ-radiation-induced DNA strand breaks in pBR 322 plasmid DNA in vitro and human peripheral blood lymphocytes ex vivo were evaluated. The protective effect of total triterpenes against γ-radiation-induced micronuclei formations in mice bone marrow cells in vivo were also evaluated. The results indicated the significant effectiveness of Ganoderma triterpenes in protecting the DNA and membrane damages consequent to the hazardous effects of radiation. The findings suggest the potential use of Ganoderma triterpenes in radio therapy. PMID:25824410

  14. Thermal annealing of radiation damage in CMOS ICs in the temperature range -140 C to +375 C

    NASA Technical Reports Server (NTRS)

    Danchenko, V.; Fang, P. H.; Brashears, S. S.

    1982-01-01

    Annealing of radiation damage was investigated in the commercial, Z- and J-processes of the RCA CD4007A ICs in the temperature range from -140 C to +375 C. Tempering curves were analyzed for activation energies of thermal annealing, following irradiation at -140 C. It was found that at -140 C, the radiation-induced shifts in the threshold potentials were similar for all three processes. The radiation hardness of the Z- and J-process is primarily due to rapid annealing of radiation damage at room temperature. In the region -140 to 20 C, no dopant-dependent charge trapping is seen, similar to that observed at higher temperatures. In the unbiased Z-process n-channels, after 1 MeV electron irradiation, considerable negative charge remains in the gate oxide.

  15. Scale-Dependent Friction and Damage Interface law: implications for effective earthquake rupture dynamics and radiation

    NASA Astrophysics Data System (ADS)

    Festa, Gaetano; Vilotte, Jean-Pierre; Raous, Michel; Henninger, Carole

    2010-05-01

    Propagation and radiation of an earthquake rupture is commonly considered as a friction dominated process on fault surfaces. Friction laws, such as the slip weakening and the rate-and-state laws are widely used in the modeling of the earthquake rupture process. These laws prescribe the traction evolution versus slip, slip rate and potentially other internal variables. They introduce a finite cohesive length scale over which the fracture energy is released. However faults are finite-width interfaces with complex internal structures, characterized by highly damaged zones embedding a very thin principal slip interface where most of the dynamic slip localizes. Even though the rupture process is generally investigated at wavelengths larger than the fault zone thickness, which should justify a formulation based upon surface energy, a consistent homogeneization, a very challenging problem, is still missing. Such homogeneization is however be required to derive the consistent form of an effective interface law, as well as the appropriate physical variables and length scales, to correctly describe the coarse-grained dissipation resulting from surface and volumetric contributions at the scale of the fault zone. In this study, we investigate a scale-dependent law, introduced by Raous et al. (1999) in the context of adhesive material interfaces, that takes into account the transition between a damage dominated and a friction dominated state. Such a phase-field formalism describes this transition through an order parameter. We first compare this law to standard slip weakening friction law in terms of the rupture nucleation. The problem is analyzed through the representation of the solution of the quasi-static elastic problem onto the Chebyshev polynomial basis, generalizing the Uenishi-Rice solution. The nucleation solutions, at the onset of instability, are then introduced as initial conditions for the study of the dynamic rupture propagation, in the case of in-plane rupture

  16. Vacuum-ultraviolet laser uses superfluid helium

    NASA Technical Reports Server (NTRS)

    Zmuidzinas, J. S.

    1979-01-01

    Vacuum ultra violet laser in wavelength around 0.800 microns is produced by using optical pumping to increase lifetimes of excited metastable molecules in super fluid helium. In method, super fluid helium is pumped electronically to produce excited HE2, and then pumped by circularly polarized 0.9096 - micron radiation to aline excited HE2 molecular spins. High power ultraviolet radiation has potential applications in molecular reaction studies, power transmission in space, and biomedical research.

  17. Strength Loss in MA-MOX Green Pellets from Radiation Damage to Binders

    SciTech Connect

    Paul A. Lessing; W.R. Cannon; Gerald W. Egeland; Larry D. Zuck; James K. Jewell; Douglas W. Akers; Gary S. Groenewold

    2013-06-01

    The fracture strength of green Minor Actinides (MA)-MOX pellets containing 75 wt.% DUO2, 20 wt. % PuO2, 3 wt. % AmO2 and 2 wt. % NpO2 was studied as a function of storage time, after mixing in the binder and before sintering, to test the effect of radiation damage on binders. Fracture strength degraded continuously over the 10 days of the study for all three binders studied: PEG binder (Carbowax 8000), microcrystalline wax (Mobilcer X) and Styrene-acrylic copolymer (Duramax B1022) but the fracture strength of Duramax B1022 degraded the least. For instance, for several hours after mixing Carbowax 8000 with MA MOX, the fracture strength of a pellet was reasonably high and pellets were easily handled without breaking but the pellets were too weak to handle after 10 days. Strength measured using diametral compression test showed strength degradation was more rapid in pellets containing 1.0 wt. % Carbowax PEG 8000 compared to those containing only 0.2 wt. %, suggesting that irradiation not only left the binder less effective but also reduced the pellet strength. In contrast the strength of pellets containing Duramax B1022 degraded very little over the 10 day period. It was suggested that the styrene portion of the Duramax B1022 copolymer provided the radiation resistance.

  18. Late skin damage in rabbits and monkeys after exposure to particulate radiations

    NASA Astrophysics Data System (ADS)

    Bergtold, D. S.; Cox, A. B.; Lett, J. T.; Su, C. M.

    Skin biopsies were taken from the central regions of the ears of New Zealand white rabbits following localized exposure of one ear of each rabbit to 530 MeV/amu Ar or 365 MeV/amu Ne ions. The unirradiated ears served as controls. Biopsies were taken also from the chests and inner thighs of rhesus monkeys after whole-body exposure to 32 MeV protons and from unirradiated control animals. The linear energy transfers (LET∞'s) for the radiations were 90 +/- 5, 35 +/- 3, and ~1.2 keV/μm, respectively. In the rabbit studies, explants were removed with a 2 mm diameter dermal punch at post-irradiation times up to five years after exposure. Similar volumes of monkey tissue were taken from skin samples excised surgically 16-18 years following proton irradiation. Fibroblast cultures were initiated from the explants and were propagated in vitro until terminal senescence (cessation of cell division) occurred. Cultures from irradiated tissue exhibited decreases in doubling potential that were dependent on radiation dose and LET∞ and seemed to reflect damage to stem cell populations. The implications of these results for astronauts exposed to heavy ions and/or protons in space include possible manifestations of residual effects in the skin many years after exposure (e.g. unsatisfactory responses to trauma or surgery).

  19. Protection from radiation-induced damage to spermatogenesis by hormone treatment

    SciTech Connect

    Kurdoglu, B.; Wilson, G.; Parchuri, N.; Ye, W.; Meistrich, M.L.

    1994-07-01

    Infertility caused by killing of the spermatogonial stem cells occurs frequently in men treated for cancer with radiotherapy and chemotherapy. We investigated whether pretreatment of rats with testosterone plus estradiol, which reversibly inhibits the completion of spermatogenesis and protects spermatogonial stem cells from procarbazine-induced damage, would also protect these cells from radiation. Adult male LBNF rats were implanted for 6 weeks with capsules containing testosterone and estradiol and then irradiated with doses from 2.5-7.0 Gy. Controls were irradiated with 1.8-3.5 Gy. Implants were removed 1 day after irradiation, and all animals were killed 10 weeks later for assessment of stem cell survival by counting repopulating tubules in histological sections and by sperm head counts. At doses of 2.5 and 3.5 Gy the repopulation indices and sperm head counts were significantly higher (P < 0.001) in the rats treated with testosterone and estradiol than in the controls. Protection factors calculated from the dose-response curves were in the range of 1.5-2.2. Elucidation of the mechanism of protection is essential to apply it to clinical situations. The fact that the spermatogonia are protected against radiation as well as procarbazine indicates that the mechanism does not involve drug delivery or metabolism. 32 refs., 3 figs.

  20. Nuclear heating, radiation damage, and waste management options for the HYLIFE-II final focus magnets

    SciTech Connect

    Latkowski, J F; Moir, R W; House, P A

    1999-08-09

    Heavy-ion fusion (HIF) designs for inertial fusion energy (XFE) power plants typically require final focusing magnets just outside the reaction chamber and blanket. Due to penetrations within the chamber and blanket, the magnets are exposed to a radiation environment. Although the magnet bores would be sized to avoid line-of-sight irradiation, the magnets still would be susceptible to nuclear heating and radiation damage from neutrons and y-rays. Additionally, the magnets must be included in waste management considerations due to neutron activation. Modified versions of the HYLIFE-II IFE power plant featuring two-sided illumination by arrays of 32 or 96 beams from each side are presented. A simple, point-of-departure quadrupole magnet design is assumed, and a three-dimensional neutronics model is created for the Flibe pocket, first wall, blanket, shield, and final two focusing magnets. This work details state-of-the-art neutronics calculations and shows that the final focus system needs to be included in the economic and environmental considerations for the driver-chamber interface of any HIF IFE power plant design.