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Sample records for high-frequency sedimentation cycles

  1. The impact of high-frequency sedimentation cycles on stratigraphic interpretation

    SciTech Connect

    Perlmutter, M.A.; Radovich, B.J.; Matthews, M.D.

    1997-01-01

    Global cyclostratigraphy, a methodology that utilizes climate change to evaluate sediment flux, characterizes the impact of sediment cycles on stratigraphy. Climatic succession, sediment yield cycles, and the phase relationship of sediment cycles to eustatic cycles are all determined in the early stages of basin analysis. Sedimentologic information is then used to assist in sequence evaluations. Climatic successions are intrinsically associated with global position (paleogeography) and are not necessarily synchronous with glacioeustatic sea-level cycles. A preliminary evaluation of the effect of climate on sediment supply from modem river systems indicates that sediment yield may vary by well over two orders of magnitude during one climate cycle. Consequently, basins in different climatic belts can have distinctly different volumes and lithologies for systems tracts that have similar base-level changes. The stratigraphic computer program Sedpak was utilized to examine the possible impact of different sedimentation cycles on sequence interpretation and reservoir forecasts. The effect of sedimentation cycles on reservoir distribution in real world sequences is demonstrated with a comparison of the Miocene section of the Surma basin, Bangladesh, and the Plio-Pleistocene section of the Gulf of Mexico. In the Surma basin, reservoirs are most likely to occur in transgressive and highstand systems tracts, while reservoirs in the Gulf of Mexico are more likely in lowstand prograding complexes.

  2. High Frequency Time-series of the Dynamic Sedimentation Processes on the Western Shelf of the Mississippi River Delta

    NASA Astrophysics Data System (ADS)

    Dail, M. B.; Corbett, D. R.; McKee, B.; Duncan, D.

    2004-12-01

    Rivers annually transport billions of tons of organic and inorganic sediment to coastal environments, making them an extremely important part of global biogeochemical cycles. However, the majority of the freshwater and suspended materials are delivered to the coastal ocean by only a few rivers. In these river-dominated ocean margins (RiOMar), sediments are deposited and re-suspended repeatedly before stable deposition. This sediment cycling is poorly understood and is critical to understanding how deltas and continental shelves, considered to be major repositories of organic carbon in marine sediments, manipulate the global carbon cycle and biogeochemical processes affecting coastal environments. During six cruises in the fall of 2003 (October, November, and December) and spring of 2004 (March, April, and May), on the shelf west of the Mississippi River Delta, sediment samples collected from cores were analyzed for particle reactive radionuclides (210Pb, 137Cs, and 234Th) to create a quantitative high frequency time-series of sediment deposition and erosion processes and evaluate the transport and fate of material on the shelf. Based on previous work completed by Corbett et al. (2004), seasonal variations in short-lived tracers could be explained by river flow and weather conditions. Inventories of the tracers collected during the fall cruises suggest increased deposition during the late summer months and that most sediment reworking and export occurs during the winter months, typically a period of low/increasing river discharge and increased weather forcing.

  3. Characterizing riverbed sediment using high-frequency acoustics 1: spectral properties of scattering

    USGS Publications Warehouse

    Buscombe, Daniel D.; Grams, Paul E.; Kaplinski, Matt A.

    2014-01-01

    Bed-sediment classification using high-frequency hydro-acoustic instruments is challenging when sediments are spatially heterogeneous, which is often the case in rivers. The use of acoustic backscatter to classify sediments is an attractive alternative to analysis of topography because it is potentially sensitive to grain-scale roughness. Here, a new method is presented which uses high-frequency acoustic backscatter from multibeam sonar to classify heterogeneous riverbed sediments by type (sand, gravel,rock) continuously in space and at small spatial resolution. In this, the first of a pair of papers that examine the scattering signatures from a heterogeneous riverbed, methods are presented to construct spatially explicit maps of spectral properties from geo-referenced point clouds of geometrically and radiometrically corrected echoes. Backscatter power spectra are computed to produce scale and amplitude metrics that collectively characterize the length scales of stochastic measures of riverbed scattering, termed ‘stochastic geometries’. Backscatter aggregated over small spatial scales have spectra that obey a power-law. This apparently self-affine behavior could instead arise from morphological- and grain-scale roughnesses over multiple overlapping scales, or riverbed scattering being transitional between Rayleigh and geometric regimes. Relationships exist between stochastic geometries of backscatter and areas of rough and smooth sediments. However, no one parameter can uniquely characterize a particular substrate, nor definitively separate the relative contributions of roughness and acoustic impedance (hardness). Combinations of spectral quantities do, however, have the potential to delineate riverbed sediment patchiness, in a data-driven approach comparing backscatter with bed-sediment observations (which is the subject of part two of this manuscript).

  4. Towards in situ and high frequency estimates of suspended sediment properties

    NASA Astrophysics Data System (ADS)

    Martínez-Carreras, Núria; Schwab, Michael Peter; Klaus, Julian; Hissler, Christophe

    2016-04-01

    Sediment properties, including sediment-associated chemical constituents and sediment physical properties (as colour), can exhibit significant variations within and between storm runoff events. However, the number of samples included in suspended sediment studies is often limited by the time consuming and expensive laboratory procedures for suspended sediment analysis after stream water sampling. This, in turn, restricts high frequency sampling campaigns to a limited number of events and reduces accuracy when aiming to estimate fluxes and loads of sediment-associated chemical constituents. Our contribution addresses the potential for portable ultraviolet-visible (UV-VIS) light spectrometers (220-730 nm) to estimate suspended sediment properties in situ and at high temporal resolution. As far as we know, these instruments have primarily been developed and used to quantify solute concentrations (e.g. DOC and NO3-N), total concentrations of dissolved and particulate forms (e.g. TOC) and turbidity. Here we argue that light absorbance values can be calibrated to estimate solely sediment properties. For our proof-of-concept experiment, we measured light absorbance at 15-min intervals at the Weierbach catchment (NW Luxembourg, 0.46 km2) from December 2013 to January 2015. We then performed a local calibration using suspended sediment loss-on-ignition (LOI) measurements (n=34). We assessed the performance of several regression models that relate light absorbance measurements with the percentage weight LOI. The robust regression method presented the lowest standard error of prediction (0.48{%}) and was selected for calibration (adjusted r2 = 0.76 between observed and predicted values). This study demonstrates that spectrometers can be used to estimate suspended sediment properties at high temporal resolution and for long time spans in a simple, non-destructive and affordable manner. The advantages and disadvantages of the method compared to traditional approaches will be

  5. Characterizing riverbed sediment using high-frequency acoustics 2: scattering signatures of Colorado River bed sediment in Marble and Grand Canyons

    USGS Publications Warehouse

    Buscombe, Daniel D.; Grams, Paul E.; Kaplinski, Matt A.

    2014-01-01

    In this, the second of a pair of papers on the statistical signatures of riverbed sediment in high-frequency acoustic backscatter, spatially explicit maps of the stochastic geometries (length- and amplitude-scales) of backscatter are related to patches of riverbed surfaces composed of known sediment types, as determined by geo-referenced underwater video observations. Statistics of backscatter magnitudes alone are found to be poor discriminators between sediment types. However, the variance of the power spectrum, and the intercept and slope from a power-law spectral form (termed the spectral strength and exponent, respectively) successfully discriminate between sediment types. A decision-tree approach was able to classify spatially heterogeneous patches of homogeneous sands, gravels (and sand-gravel mixtures), and cobbles/boulders with 95, 88, and 91% accuracy, respectively. Application to sites outside the calibration, and surveys made at calibration sites at different times, were plausible based on observations from underwater video. Analysis of decision trees built with different training data sets suggested that the spectral exponent was consistently the most important variable in the classification. In the absence of theory concerning how spatially variable sediment surfaces scatter high-frequency sound, the primary advantage of this data-driven approach to classify bed sediment over alternatives is that spectral methods have well understood properties and make no assumptions about the distributional form of the fluctuating component of backscatter over small spatial scales.

  6. Characterizing riverbed sediment using high-frequency acoustics: 2. Scattering signatures of Colorado River bed sediment in Marble and Grand Canyons

    NASA Astrophysics Data System (ADS)

    Buscombe, D.; Grams, P. E.; Kaplinski, M. A.

    2014-12-01

    In this, the second of a pair of papers on the statistical signatures of riverbed sediment in high-frequency acoustic backscatter, spatially explicit maps of the stochastic geometries (length and amplitude scales) of backscatter are related to patches of riverbed surfaces composed of known sediment types, as determined by georeferenced underwater video observations. Statistics of backscatter magnitudes alone are found to be poor discriminators between sediment types. However, the variance of the power spectrum and the intercept and slope from a power law spectral form (termed the spectral strength and exponent, respectively) successfully discriminate between sediment types. A decision tree approach was able to classify spatially heterogeneous patches of homogeneous sands, gravels (and sand-gravel mixtures), and cobbles/boulders with 95, 88, and 91% accuracy, respectively. Application to sites outside the calibration and surveys made at calibration sites at different times were plausible based on observations from underwater video. Analysis of decision trees built with different training data sets suggested that the spectral exponent was consistently the most important variable in the classification. In the absence of theory concerning how spatially variable sediment surfaces scatter high-frequency sound, the primary advantage of this data-driven approach to classify bed sediment over alternatives is that spectral methods have well-understood properties and make no assumptions about the distributional form of the fluctuating component of backscatter over small spatial scales.

  7. Quantifying Surface Processes and Stratigraphic Characteristics Resulting from Large Magnitude High Frequency and Small Magnitude Low Frequency Relative Sea Level Cycles: An Experimental Study

    NASA Astrophysics Data System (ADS)

    Yu, L.; Li, Q.; Esposito, C. R.; Straub, K. M.

    2015-12-01

    Relative Sea-Level (RSL) change, which is a primary control on sequence stratigraphic architecture, has a close relationship with climate change. In order to explore the influence of RSL change on the stratigraphic record, we conducted three physical experiments which shared identical boundary conditions but differed in their RSL characteristics. Specifically, the three experiments differed with respect to two non-dimensional numbers that compare the magnitude and periodicity of RSL cycles to the spatial and temporal scales of autogenic processes, respectively. The magnitude of RSL change is quantified with H*, defined as the peak to trough difference in RSL during a cycle divided by a system's maximum autogenic channel depth. The periodicity of RSL change is quantified with T*, defined as the period of RSL cycles divided by the time required to deposit one channel depth of sediment, on average, everywhere in the basin. Experiments performed included: 1) a control experiment lacking RSL cycles, used to define a system's autogenics, 2) a high magnitude, high frequency RSL cycles experiment, and 3) a low magnitude, low frequency cycles experiment. We observe that the high magnitude, high frequency experiment resulted in the thickest channel bodies with the lowest width-to-depth ratios, while the low magnitude, long period experiment preserves a record of gradual shoreline transgression and regression producing facies that are the most continuous in space. We plan to integrate our experimental results with Delft3D numerical experiments models that sample similar non-dimensional characteristics of RSL cycles. Quantifying the influence of RSL change, normalized as a function of the spatial and temporal scales of autogenic processes will strengthen our ability to predict stratigraphic architecture and invert stratigraphy for paleo-environmental conditions.

  8. High-frequency time-series of the dynamic sedimentation processes on the western shelf of the Mississippi River Delta

    NASA Astrophysics Data System (ADS)

    Reide Corbett, D.; Dail, Michael; McKee, Brent

    2007-06-01

    Multiple box cores were collected on the continental shelf in the Mississippi Deltaic Region adjacent to Southwest Pass and analyzed for particle reactive radionuclides 234Th and 7Be to examine seasonal sediment dynamics associated with variations of river discharge and hydrodynamics. Three stations located along a line west of Southwest Pass were cored and reoccupied in October, November, and December of 2003 and March, April, and May of 2004. High-frequency sampling (˜monthly) comparable to the short half-life of the radiotracers ( 234Th t1/2=24.1 d; 7Be t1/2=53.3) enabled us to isolate the relative influence that various forcing agents (river discharge, waves, currents) had on sediment inventories of 7Be and 234Th. In addition, the primary source of 7Be (fluvial) differs from 234Th (marine), providing further insight into processes affecting sediment transport and supply. Monthly 7Be inventories showed a significant positive relationship to river discharge ( P=0.03) proximal to Southwest Pass. Sites further from Southwest Pass exhibited little to no relationship between 7Be inventories and river flow. At these sites, monthly 7Be inventories demonstrated a significant positive relationship with average wave orbital velocity ( P<0.01). During our sampling period, the transport of 7Be-rich sediments to sites located on the middle to outer shelf were dependent on sea conditions not river discharge. Relatively high wave orbital velocities potentially allow particles to remain in suspension longer and travel further distances before initial deposition. In addition, 234Th inventories showed evidence of sediment focusing during periods of high wave orbital velocities.

  9. High-frequency thermal-electrical cycles for pyroelectric energy conversion

    SciTech Connect

    Bhatia, Bikram; Damodaran, Anoop R.; Cho, Hanna; Martin, Lane W.; King, William P.

    2014-11-21

    We report thermal to electrical energy conversion from a 150 nm thick BaTiO{sub 3} film using pyroelectric cycles at 1 kHz. A microfabricated platform enables temperature and electric field control with temporal resolution near 1 μs. The rapid electric field changes as high as 11 × 10{sup 5 }kV/cm-s, and temperature change rates as high as 6 × 10{sup 5 }K/s allow exploration of pyroelectric cycles in a previously unexplored operating regime. We investigated the effect of phase difference between electric field and temperature cycles, and electric field and temperature change rates on the electrical energy generated from thermal-electrical cycles based on the pyroelectric Ericsson cycle. Complete thermodynamic cycles are possible up to the highest cycle rates tested here, and the energy density varies significantly with phase shifts between temperature and electric field waveforms. This work could facilitate the design and operation of pyroelectric cycles at high cycle rates, and aid in the design of new pyroelectric systems.

  10. Zero Voltage Soft Switching Duty Cycle Pulse Modulated High Frequency Inverter-Fed

    NASA Astrophysics Data System (ADS)

    Ishitobi, Manabu; Matsushige, Takayuki; Nakaoka, Mutsuo; Bessyo, Daisuke; Omori, Hideki; Terai, Haruo

    The utility grid voltage of commercial AC power source in Japan and USA is 100V, but in other Asian and European countries, it is 220V. In recent years, in Japan 200V outputted single-phase three-wire system begins to be used for high power applications. In 100V utility AC power applications and systems, an active voltage clamped quasi-resonant inverter circuit topology sing IGBTs has been effectively used so far for the consumer microwave oven. In this paper, presented is a half bridge type voltage-clamped asymmetrical soft switching PWM high-frequency inverter type AC-DC converter using IGBTs which is designed for consumer magnetron drive used as the consumer microwave oven in 200V utility AC power system. The zero voltage soft switching inverter treated here can use the same power rated switching semiconductor devices and three-winding high frequency transformer as those of the active voltage clamped quasi-resonant inverter using the IGBTs that has already been used for 100V utility AC power source. The operating performances of the voltage source single ended push pull (SEPP) type soft switching PWM inverter are evaluated and discussed for 100V and 200V common use consumer microwave oven. The harmonic line current components in the utility AC power side of the AC-DC power converter with ZVS-PWM SEPP inverter are reduced and improved on the basis of sine wave like pulse frequency modulation and sine wave like pulse width modulation for the utility AC voltage source.

  11. High-frequency oscillations in human and monkey neocortex during the wake-sleep cycle.

    PubMed

    Le Van Quyen, Michel; Muller, Lyle E; Telenczuk, Bartosz; Halgren, Eric; Cash, Sydney; Hatsopoulos, Nicholas G; Dehghani, Nima; Destexhe, Alain

    2016-08-16

    Beta (β)- and gamma (γ)-oscillations are present in different cortical areas and are thought to be inhibition-driven, but it is not known if these properties also apply to γ-oscillations in humans. Here, we analyze such oscillations in high-density microelectrode array recordings in human and monkey during the wake-sleep cycle. In these recordings, units were classified as excitatory and inhibitory cells. We find that γ-oscillations in human and β-oscillations in monkey are characterized by a strong implication of inhibitory neurons, both in terms of their firing rate and their phasic firing with the oscillation cycle. The β- and γ-waves systematically propagate across the array, with similar velocities, during both wake and sleep. However, only in slow-wave sleep (SWS) β- and γ-oscillations are associated with highly coherent and functional interactions across several millimeters of the neocortex. This interaction is specifically pronounced between inhibitory cells. These results suggest that inhibitory cells are dominantly involved in the genesis of β- and γ-oscillations, as well as in the organization of their large-scale coherence in the awake and sleeping brain. The highest oscillation coherence found during SWS suggests that fast oscillations implement a highly coherent reactivation of wake patterns that may support memory consolidation during SWS. PMID:27482084

  12. New Seasonal Shift in In-Stream Diurnal Nitrate Cycles Identified by Mining High-Frequency Data.

    PubMed

    Aubert, Alice H; Breuer, Lutz

    2016-01-01

    The recent development of in-situ monitoring devices, such as UV-spectrometers, makes the study of short-term stream chemistry variation relevant, especially the study of diurnal cycles, which are not yet fully understood. Our study is based on high-frequency data from an agricultural catchment (Studienlandschaft Schwingbachtal, Germany). We propose a novel approach, i.e. the combination of cluster analysis and Linear Discriminant Analysis, to mine from these data nitrate behavior patterns. As a result, we observe a seasonality of nitrate diurnal cycles, that differs from the most common cycle seasonality described in the literature, i.e. pre-dawn peaks in spring. Our cycles appear in summer and the maximum and minimum shift to a later time in late summer/autumn. This is observed both for water- and energy-limited years, thus potentially stressing the role of evapotranspiration. This concluding hypothesis on the role of evapotranspiration on nitrate stream concentration, which was obtained through data mining, broadens the perspective on the diurnal cycling of stream nitrate concentrations. PMID:27073838

  13. New Seasonal Shift in In-Stream Diurnal Nitrate Cycles Identified by Mining High-Frequency Data

    PubMed Central

    2016-01-01

    The recent development of in-situ monitoring devices, such as UV-spectrometers, makes the study of short-term stream chemistry variation relevant, especially the study of diurnal cycles, which are not yet fully understood. Our study is based on high-frequency data from an agricultural catchment (Studienlandschaft Schwingbachtal, Germany). We propose a novel approach, i.e. the combination of cluster analysis and Linear Discriminant Analysis, to mine from these data nitrate behavior patterns. As a result, we observe a seasonality of nitrate diurnal cycles, that differs from the most common cycle seasonality described in the literature, i.e. pre-dawn peaks in spring. Our cycles appear in summer and the maximum and minimum shift to a later time in late summer/autumn. This is observed both for water- and energy-limited years, thus potentially stressing the role of evapotranspiration. This concluding hypothesis on the role of evapotranspiration on nitrate stream concentration, which was obtained through data mining, broadens the perspective on the diurnal cycling of stream nitrate concentrations. PMID:27073838

  14. Evolution of High-Frequency Turbulence During Limit-Cycle Oscillations on DIII-D

    NASA Astrophysics Data System (ADS)

    Rost, J. C.; Marinoni, A.; Davis, E. M.; Porkolab, M.; Burrell, K. H.

    2014-10-01

    Limit-cycle oscillations (LCO) can provide insight into the interplay between shear and turbulence in triggering the H-mode transition. The Phase Contrast Imaging (PCI) diagnostic on DIII-D is particularly sensitive to density fluctuations in the highly sheared flow in the H-mode/LCO edge due to sensitivity to finite radial wave number (kr ~kθ) and large bandwidth (10 kHz < f < 2 MHz). Each roughly 1 ms oscillation in the LCO (10s of ms) exhibits a period of highly Doppler shifted, highly sheared turbulence which terminates at a burst of low-f turbulence. As the Doppler backscattering (DBS) diagnostic records a gradual increase in fluctuation amplitude rather than a burst, the PCI signal can be explained by a sudden decrease in radial correlation length caused by a burst in zonal flows. Both diagnostics are consistent with results of 1D models. Comparison of LCOs of different durations reveals a threshold-like behavior in mean flow. Work supported by the US DOE under DE-FG02-94ER54235 and DE-FC02-04ER54698.

  15. How important is subsidence in evaluating high frequency cycles in the interior of isolated carbonate platforms?

    SciTech Connect

    Lomando, A.J.; Ginsburg, R.

    1995-08-01

    Differential regional subsidence can play a strong role in determining facies composition of adjacent isolated platforms. In the majority of platform oil & gas fields, reservoir architecture is dominated by the composition and stratial geometries in the platform interiors rather than the platform rims. Most work to develop analogues from modern reef-rimmed isolated platforms has focused on the platform margins where reef growths rates are capable of keeping pace with the Holocene sea level rise plus tectonic subsidence. We have focused on platform interiors where accumulation rate and style may be more sensitive to accommodation space generated by regional passive margin subsidence. The Belize platforms are located on a series of parallel ridges which extend progressively eastward, farther from the regional subsidence hingeline near the coast. Greater regional subsidence is reflected in the open, deeper, patch reef and sand rich platform interiors in the outermost platform (Lighthouse and Glovers Platform) in comparison to the mud-dominated interior within the innermost platform (Turneffe), which has filled up due to lesser subsidence rate. The facies response to a portion of a single eustatic cycle produces a {open_quotes}keep-up{close_quotes} transgressive systems tract appearance at Lighthouse and Glovers but a {open_quotes}choked-up{close_quotes} high stand or regressive systems tract appearance within Turneffe. Chinchorro Bank, offshore Yucatan, is a special case where subsidence changes along the length of the platforms. The entire windward margin has a well developed reef system which has uniformly kept pace with the Holocene transgression. The northern platform interior contains a patch reef/sand rich character similar to Lighthouse Platform whereas the southern platform interior is {open_quotes}drowning{close_quotes} due to subsidence along a series of northwest trending faults which downstep southward.

  16. Phase reduction of a limit cycle oscillator perturbed by a strong amplitude-modulated high-frequency force

    NASA Astrophysics Data System (ADS)

    Pyragas, Kestutis; Novičenko, Viktor

    2015-07-01

    The phase reduction method for a limit cycle oscillator subjected to a strong amplitude-modulated high-frequency force is developed. An equation for the phase dynamics is derived by introducing a new, effective phase response curve. We show that if the effective phase response curve is everywhere positive (negative), then an entrainment of the oscillator to an envelope frequency is possible only when this frequency is higher (lower) than the natural frequency of the oscillator. Also, by using the Pontryagin maximum principle, we have derived an optimal waveform of the perturbation that ensures an entrainment of the oscillator with minimal power. The theoretical results are demonstrated with the Stuart-Landau oscillator and model neurons.

  17. Evidence of Increasing Acoustic Emissivity at High Frequency with Solar Cycle 23 in Sun-as-a-star Observations

    SciTech Connect

    Simoniello, R.; Finsterle, W.

    2009-09-16

    We used long high-quality unresolved (Sun-as-a-star observations) data collected by GOLF and VIRGO instruments on board the ESA/NASA SOHO satellite to investigate the amplitude variation with solar cycle 23 in the high-frequency band (5.7<{nu}<6.3 mHz). We found an enhancement of acoustic emissivity over the ascending phase of about 18{+-}3 in velocity observations and a slight enhancement of 3{+-}2 in intensity. Mode conversion from fast acoustic to fast magneto-acoustic waves could explain the enhancement in velocity observations. These findings open up the possibility to apply the same technique to stellar intensity data, in order to investigate stellar-magnetic activity.

  18. Sulfur cycling in freshwater sediments

    NASA Technical Reports Server (NTRS)

    Klug, M. J.

    1985-01-01

    Organic sulfur containing compounds represent greater than 80% of the total sulfur in sediments of eutrophic freshwater lakes. Although sedimentary sulfur is predominantly in the form of organic compounds, more sulfur is transformed by sulfate reduction than by any other process. Rates of sulfate reduction in these sediments average 7 mmol/sq m/day. This rate is 19 times greater than the net rate of production of inorganic sulfur from organic compounds on an annual basis.

  19. Interpreting Organic Carbon Cycling from High-Frequency Stream FDOM, Turbidity, and CO2 Measurements at the USGS WEBB Watersheds

    NASA Astrophysics Data System (ADS)

    Shanley, J. B.; Saraceno, J.; Pellerin, B. A.; Dornblaser, M.; Clow, D. W.; Aulenbach, B. T.; Walker, J. F.; Aiken, G.

    2013-12-01

    At the five forested and/or alpine headwater sites of the U.S. Geological Survey (USGS) Water, Energy, and Biogeochemical Budgets (WEBB) program, we measure fluorescing dissolved organic matter (FDOM), turbidity, and dissolved CO2 at high frequency with in-stream sensors. Goals of this effort are to compute accurate stream fluxes of DOC, POC, and CO2 and compare them to conventional sample-based approaches, as well as to exploit the variability in the signals - over temporal scales from event to season - to infer processes controlling these carbon phases in the watershed and the stream. We take discrete samples over a range of hydrologic conditions to verify the field measurements and test the proxy power of FDOM for DOC, and turbidity for POC. After correcting FDOM for water temperature, turbidity, and the inner filter effect (attenuation of signal by DOC itself), field and laboratory FDOM values agree closely and DOC-FDOM and POC-turbidity relations typically have an r2 > 0.9. We will present four examples of interpretation: (1) Diurnal cycles of FDOM occur during the cold-water snowmelt period before canopy leafout, underscoring the importance of light to stimulate algal and microbial activity; (2) The nature of hysteresis in the FDOM-stream discharge relation (e.g. size and direction of hysteretic loop) can reveal DOM sources and travel times within the catchment/stream system; (3) Seasonal FDOM patterns reveal the shifting importance of flushing in spring versus new production in summer and especially autumn (leaf fall); and (4) Event and seasonal shifts in stream CO2 concentrations suggest shifts in relative contributions from discrete zones within the shallow aquifer.

  20. The effective Q values inferred from the high-frequency decay parameter for the sediments in Taipei basin, Taiwan

    NASA Astrophysics Data System (ADS)

    Huang, Ming-Wey; Wen, Kuo-Liang; Chang, Chi-Ling; Liu, Sheu-Yien

    2016-04-01

    In this study, the high-frequency decay parameter κ, proposed by Anderson and Hough (1984), are measured from the seismograms recorded by stations, which installed in the Taipei basin. The spectral amplitudes decay exponentially with frequency, f, which can be formulated as A(f)=A_0e‑πκf, for f > fe, where A(f) is the spectral amplitude, and A0 depends on the earthquake source and epicenter distance, and the value of κ is independent of frequency, unit in second. The time windows applied to seismograms are suggested to be shear waves that are transformed to spectra by the technique of Fourier transform. The seismograms from the downhole array in Taipei Basin by Academia Sinica since 1992, provide a good opportunity to estimate the attenuation factor of the sedimentary strata over the Tertiary base rock beneath the Taipei basin (Wang et al., 2004). The seismograms of 23 earthquakes with magnitude ranges of 5.1-7.1 over the period of 2003-2010 at 9 downhole array stations are taken into calculation of the κ values for the shear waves. The results show that the κ values vary with depth and are in the range of 0.009-0.095 sec. The averaged Δκ values from observations range +/- 0.02 seconds respective to Δκ values at surface of each of station. The effective Q values for the sedimentary layers are inferred from the varied Δκ at each downhole stations following the evaluation method of 1-D analytical transfer function (Safak, 1995).

  1. Temporal Variability and Annual Fluxes of Water, Sediment and Particulate Phosphorus from a Headwater River in the Tropical Andes: Results from a High-frequency Monitoring Program

    NASA Astrophysics Data System (ADS)

    Wemple, B. C.; Schloegel, C.

    2015-12-01

    The Mazar River Project, a high-frequency hydrological monitoring program, aims to generate ecohydrological information to inform watershed management in high-mountain areas of southern Ecuador. Rapid development of hydropower, accompanied by new and improved road networks, has resulted in swift changes in land-use and land cover in Ecuador's tropical Andes, all of which underscore the need for detailed information on flow and sediment production from these river systems. National and regional payment for the protection of ecosystem services (PES) programs seek to target critical areas, such as these, for watershed conservation, but are often informed by minimal information on sustainable flows and impacts of land use activities. As part of a program to inform conservation and sustainable water management in the region, we established a hydrological monitoring station in southern Ecuador on the Mazar River, a tributary of the Paute River Basin, situated on the eastern Andean cordillera. The station is equipped with sensors to continuously monitor stream stage and turbidity and an automated sampler for event-based collection of stream water samples, providing high frequency data that reduces the uncertainty of observations. Here, we report observations of continuous runoff and turbidity over the first year of observation, present relationships between turbidity and concentrations of total suspended solids (TSS) and total particulate phosphorus (TP), and provide estimates of annual loads of TSS and TP. Runoff was highly variable over the monitoring period with flows ranging from less than 3 m3/s during baseflow to nearly 80 m3/s during the flood of record. During measured storm events, TSS exceeded 1000 mg/l with maximum measured concentrations exceeding 13 g/l during storm peaks. Turbidity was highly correlated with TSS, which was in turn highly correlated with TP, providing a robust data set for load estimation. We compare our results to other montane rivers in the

  2. Pt/C catalyst degradation in proton exchange membrane fuel cells due to high-frequency potential cycling induced by switching power converters

    NASA Astrophysics Data System (ADS)

    Uno, Masatoshi; Tanaka, Koji

    Proton exchange membrane fuel cells are operated using switching power converters that produce high-frequency ripple currents. These ripples cause high-frequency potential cycling of cells, which is believed to lead premature deterioration in the electrochemical surface area (ECA) of Pt/C catalysts. The qualitative relationship between ECA losses and the frequency of potential cycling was investigated in the range of 1 Hz to 1 kHz. For frequencies higher than 100 Hz, ECA losses were comparable with those at the potential hold condition. However, for lower frequencies, ECA decreased significantly with decreasing frequency. TEM observations showed that there was marked Pt particle growth for the 1-Hz cycling condition, whereas particle size distributions at 100 Hz and potential hold conditions were comparable. The currents associated with Pt oxidation and reduction during potential cycling were also investigated at various potentials and frequencies, and the charges associated with Pt loss (Δ Q) were determined by integrating the measured current. A correlation between the ECA trend and Δ Q was observed. The results obtained in this study are considered informative for electrical engineering research, because it relates to the design of switching power converters that do not negatively influence the Pt/C catalyst durability.

  3. Relevance of different spectral techniques to describe estuarine suspended sediment dynamics based on a high-frequency, long-term turbidity dataset

    NASA Astrophysics Data System (ADS)

    Jalón Rojas, Isabel; Schmidt, Sabine; Sottolichio, Aldo

    2015-04-01

    Sediment dynamics in estuaries are complex and strongly variable over time scales ranging from seconds to years. Various forcings (turbulence, tides, river inflow, wind waves, morphological and climatic changes) may cause the temporal and spatial variability of suspended sediment (SS) concentrations. The evaluation of these SS dynamics by in-situ measurements have traditionally faced three difficulties: (1) the quantification of low-frequency variability that requires continuous measures over long time periods; (2) inevitable gaps in data limiting the post-processing; (3) the need for recording other environmental variables in the same period and at a coherent sampling frequency. To record a high-frequency and long-term turbidity dataset, an automatic monitoring network (MAGEST) has been implemented in the Gironde estuary, a macrotidal and highly turbid system in the South-West France, in 2004. This 10-year turbidity time series is rather unique in European estuaries, enabling the evaluation of SS dynamics at all the significant time scales in one single analysis of the dataset. To achieve this, several methodologies of data analysis using different approaches are available, but their relevance, especially for the more recently developed ones, is almost unexplored. In this work, we present the test of four spectral techniques to the analysis of a high-frequency turbidity time series of an estuary such as the Gironde, to discuss advantages and limitations of each method. We compare the Power Spectral Analysis (PSA), the Singular Spectral Analysis (SSA), the Wavelet Transform (WT) and the Empirical Mode Decomposition (EMD). Advantages and limitations of each method are evaluated on the basis of five criteria: efficiency for incomplete time series, appropriateness for time-varying analysis, ability to recognize processes without the need of complementary environmental variables, capacity to calculate the relative importance of processes, and capacity to identify long

  4. High-power high-frequency-induced Langmuir turbulence in the smooth ionosphere at Arecibo. II. Low duty cycle, altitude-resolved, observations

    SciTech Connect

    Cheung, P. Y.; Sulzer, M. P.; DuBois, D. F.; Russell, D. A.

    2001-03-01

    This is the second of two papers comprising a theoretical and observational study of new, altitude-resolved, observations at Arecibo of Langmuir turbulence induced in the ionosphere by a new, more powerful, high frequency heater operated at very low duty cycles. Altitude resolution of 150 m in incoherent scatter radar spectra is made possible by the coded-long-pulse method. Here we present the first observation at Arecibo of the well-developed parametric decay instability and the Langmuir decay instability cascade features in the Thomson scatter radar power spectrum, of the plasma line, at the unmodified matching altitudes under near-cold start conditions. The dependence of the plasma line spectra on altitude, pump power, and density scale length have been studied. The temporal growth and saturation of the spectra during heating and the decay of the spectra in the afterglow of heating has also been studied in detail. Comparisons are made here with the theoretical predictions of the companion paper I [DuBois , Phys. Plasmas 8, 791 (2001)]. From these comparisons and a comparison with recent observations at both Arecibo and Tromso, we conclude that all the predictions of modern Langmuir turbulence theory for the radar spectral signatures of the turbulence in a smooth ionosphere have now been verified.

  5. TRANSIENT BIOGEOCHEMICAL CYCLING AND SEDIMENT OXYGEN DEMAND

    EPA Science Inventory

    Through this research, the effects of variable sediment accumulation and oxygen concentration on SOD and soluble chemical fluxes will be quantified. This study will enable correct estimates of “diffuser-induced” SOD to be made that will facilitate appropriate desig...

  6. High frequency peritidal cycles of the upper Araras Group: Implications for disappearance of the neoproterozoic carbonate platform in southern Amazon Craton

    NASA Astrophysics Data System (ADS)

    Rudnitzki, Isaac Daniel; Romero, Guilherme Raffaeli; Hidalgo, Renata; Nogueira, Afonso Cesar Rodrigues

    2016-01-01

    The Araras Group is an extensive carbonate platform developed at the southeastern margin of the Amazon Craton during the Neoproterozoic. The Nobres Formation corresponds to the upper unit of the Neoproterozoic Araras Group. It is exposed in road cuts and quarries in the Northern Paraguay Belt, and is characterized by meter-scale shallowing upward cycles. Forty-four fourth-to fifth-order parasequence cycles are enclosed into three third order sequences/megacycles, unconformably overlain by siliciclastic deposits of the Alto Paraguay Group. The cycles are generally of peritidal type, limited by exposure surfaces composed of asymmetrical tidal flat/sabkha lithofacies in the basal Nobres Formation. They consist of fine dolostone, intraclastic dolostones with megaripples, stromatolites biostrome, sandy dolostone with enterolithic structures and silicified evaporite molds. Upsection, the cycles progressively become symmetrical, comprising arid tidal flat deposits with abundant stromatolite biostrome, fine-grained sandstone and rare evaporitic molds. The stacking patterns for hundreds of meters indicate continuous and recurrent generation of accommodation space, probably triggered by subsidence concomitant with relative sea-level changes. Palynomorphs found in the upper part of Nobres Formation comprehend spheroidal forms, such as Leiospharidia, rare filamentous and acanthomorphous acritarchs, mostly Tanarium correlated to the Ediacaran Complex Acantomorph Palynoflora of ˜580-570 Ma. Previous data of carbon isotopes and paleogeographic reconstructions, and also the presence of evaporites and storm-influenced deposits in the Araras Group, suggest a wet to tropical setting for Amazonia during the Mid-Ediacaran, which is incompatible with previous claims for Gaskiers-related glacial sedimentation in the region. During the final stages of evolution of the Araras carbonate platform, a progressive input of terrigenous has occurred in the peritidal setting likely due tectonic

  7. Sulfur and carbon cycling in organic-rich marine sediments

    NASA Technical Reports Server (NTRS)

    Martens, C. S.

    1985-01-01

    Nearshore, continental shelf, and slope sediments are important sites of microbially mediated carbon and sulfur cycling. Marine geochemists investigated the rates and mechanisms of cycling processes in these environments by chemical distribution studies, in situ rate measurements, and steady state kinetic modeling. Pore water chemical distributions, sulfate reduction rates, and sediment water chemical fluxes were used to describe cycling on a ten year time scale in a small, rapidly depositing coastal basin, Cape Lookout Bight, and at general sites on the upper continental slope off North Carolina, U.S.A. In combination with 210 Pb sediment accumulation rates, these data were used to establish quantitative carbon and sulfur budgets as well as the relative importance of sulfate reduction and methanogeneis as the last steps in the degradation of organic matter.

  8. Manganese cycles in Arctic marine sediments - Climate signals or diagenesis?

    NASA Astrophysics Data System (ADS)

    März, C.; Stratmann, A.; Eckert, S.; Schnetger, B.; Brumsack, H.-J.

    2009-04-01

    In comparison to sediments from other parts of the world ocean, the inorganic geochemistry of Arctic Ocean sediments is poorly investigated. However, marked light to dark brown layers are well-known features of Quaternary Arctic sediments, and have been related to variable Mn contents. Brown layers represent intervals relatively rich in Mn (often > 1 wt.%), while yellowish-greyish intervals contain less Mn. As these brown layers are widespread in pelagic Quaternary deposits of the Arctic Ocean, there are attempts to use them as stratigraphic, age-equivalent marker horizons that are genetically related to global climate changes (e.g. Jakobsson et al., 2000; Löwemark et al., 2008). In the Arctic Ocean, other conventional stratigraphic methods often fail, therefore the use of Mn-rich layers as a chemostratigraphic tool seems to be a promising approach. However, several inorganic-geochemical and modelling studies of Mn cycles in the Arctic as well as in other parts of the world ocean have shown that multiple Mn layers in marine sediments can be created by non-steady state diagenetic processes, i.e. secondary Mn redistribution in the sediment due to microbially mediated dissolution-reprecipitation reactions (e.g. Li et al., 1969; Gobeil et al., 1997; Burdige, 2006; Katsev et al., 2006). Such biogeochemical processes can lead to rapid migration or fixation of redox boundaries in the sediment, resulting in the formation or (partial) destruction of metal-rich layers several thousands of years after sediment deposition. As this clearly would alter primary paleoenvironmental signals recorded in the sediments, we see an urgent need to unravel the real stratigraphic potential of Arctic Mn cycles before they are readily established as standard tools. For this purpose, we are studying Mn cycles in Arctic Ocean sediments recovered during R/V Polarstern expedition ARK XXIII/3 on the Mendeleev Ridge (East Siberian Sea). First results of pore water and sediment composition

  9. Effects of sediments on the reproductive cycle of corals.

    PubMed

    Jones, R; Ricardo, G F; Negri, A P

    2015-11-15

    Dredging, river plumes and natural resuspension events can release sediments into the water column where they exert a range of effects on underlying communities. In this review we examine possible cause-effect pathways whereby light reduction, elevated suspended sediments and sediment deposition could affect the reproductive cycle and early life histories of corals. The majority of reported or likely effects (30+) were negative, including a suite of previously unrecognized effects on gametes. The length of each phase of the life-cycle was also examined together with analysis of water quality conditions that can occur during a dredging project over equivalent durations, providing a range of environmentally relevant exposure scenarios for future testing. The review emphasizes the need to: (a) accurately quantify exposure conditions, (b) identify the mechanism of any effects in future studies, and (c) recognize the close interlinking of proximate factors which could confound interpretation of studies. PMID:26384866

  10. Iron cycling microbial communities in sediments of the Baltic Sea

    NASA Astrophysics Data System (ADS)

    Reyes, Carolina; Delwig, Olaf; Noriega-Ortega, Beatriz; Dähnke, Kirstin; Böttcher, Michael E.; Friedrich, Michael W.

    2014-05-01

    The biogeochemical cycling of iron is a key early diagenetic process. However, limited information exists about the diversity and metabolic pathways of microorganisms linked to iron cycling in marine sediments. The goal of this study was to determine the bacterial community diversity in sediments showing ongoing dissimilatory iron reduction using 454-pyrosequencing as a first step in characterizing microorganisms potentially involved in iron reduction. For this purpose, two 35 cm cores were sampled from ferruginous sediments in the Skagerrak (SK) and the Bothnian Bay (BB) from the North-Sea Baltic Sea and the northern Baltic Sea respectively. Pore water profiles showed Fe2+ and Mn2+ levels of ~140-150 µM throughout the core below a 6 cm thick oxidized surface layer in SK sediments and ~300 µM below a 2 cm thick surface layer in BB sediments. Dissolved sulphide levels were below detection in both sediments. No significant depletion of SO42- occurred at both sites, further supported by stable S and O isotope analyses of dissolved sulfate at SK site. Only very minor net sulfate reduction is suggested here from the trend in sulphur isotope signatures, in agreement with previously reported gross microbial sulphate rate measurements (Canfield et al., 1993;GCA). Based on these biogeochemical constraints, Fe reduction in the studied sediments is therefore dominated by microbial dissimilatory iron reduction, while cryptic Fe-S-cycling can be largely excluded. 16S rRNA gene sequences indicate Proteobacteria as the dominating microbial group in these sediments. Potential iron and manganese reducing bacteria included Geobacteraceae, Pelobacteraceae, Shewanellaceae, and Oceanospirillales. Additionally, Actinobacteria and Bacteroidetes were present. Also, Fe-oxidizers were present and their occurrence correlated in depth with a Fe-oxide-rich layer, most likely a former buried Fe-oxidation front. Gene sequences point to the presence of Mariprofundus in SK sediments and

  11. SULFUR CYCLING IN THALASSIA TESTUDINUM SEAGRASS BED SEDIMENTS

    EPA Science Inventory

    Quarles, Robert L., Jessica A. Rivord and Richard Devereux. In press. Sulfur Cycling in Thalassia testudinum Seagrass Bed Sediments (Abstract). To be presented at the SWS/GERS Fall Joint Society Meeting: Communication and Collaboration: Coastal Systems of the Gulf of Mexico and S...

  12. Self-sedimentation of fossil phytoplankton blooms, laminated hemipelagic sediments and the oceanic carbon cycle

    SciTech Connect

    Grimm, K.A.; Lange, C.B.

    1996-12-31

    The flux of phytoplankton-derived organic carbon from the surface ocean to the deep sea and underlying sediments is a nonuniform process that significantly impacts biogeochemical cycles, atmospheric pCO{sub 2} / O{sub 2} and organic carbon enrichment in marine sediments. Some marine phytoplankton actively drive the sedimentation process by the formation of sticky transparent gels which facilitate aggregation, rapid sinking and efficient export flux. Here we present fossil evidence of unfragmented, low-diversity phytoplankton assemblages preserved as sedimentary laminations and as preserved aggregates that are attributable to a similar phytoplankton-driven sedimentary mechanism, here termed {open_quotes}self-sedimentation{close_quotes}. Heterogeneities in the texture and/or composition of sediment supply are necessary for the production of laminatedhemipelagic sediments; the absence of hydraulic and biological reworking permits preservation of these sedimentary laminae. Distinctly-laminated core intervals are characterized by large compositional contrasts between adjacent laminae; many such high-bimodality couplets are attributable to self-sedimentation of phytoplankton blooms. Self-sedimentation propels the formation of some conspicuous hemipelagic sedimentary laminations and results in efficient carbon and opal flux to the sediments. These records suggest that phytoplankton-mediated changes in the efficiency of the biological carbon pump may govern many accumulations of organic-rich hydrocarbon source rock as well as many abrupt changes in atmospheric pCO{sub 2} and climate.

  13. Self-sedimentation of fossil phytoplankton blooms, laminated hemipelagic sediments and the oceanic carbon cycle

    SciTech Connect

    Grimm, K.A. ); Lange, C.B. )

    1996-01-01

    The flux of phytoplankton-derived organic carbon from the surface ocean to the deep sea and underlying sediments is a nonuniform process that significantly impacts biogeochemical cycles, atmospheric pCO[sub 2] / O[sub 2] and organic carbon enrichment in marine sediments. Some marine phytoplankton actively drive the sedimentation process by the formation of sticky transparent gels which facilitate aggregation, rapid sinking and efficient export flux. Here we present fossil evidence of unfragmented, low-diversity phytoplankton assemblages preserved as sedimentary laminations and as preserved aggregates that are attributable to a similar phytoplankton-driven sedimentary mechanism, here termed [open quotes]self-sedimentation[close quotes]. Heterogeneities in the texture and/or composition of sediment supply are necessary for the production of laminatedhemipelagic sediments; the absence of hydraulic and biological reworking permits preservation of these sedimentary laminae. Distinctly-laminated core intervals are characterized by large compositional contrasts between adjacent laminae; many such high-bimodality couplets are attributable to self-sedimentation of phytoplankton blooms. Self-sedimentation propels the formation of some conspicuous hemipelagic sedimentary laminations and results in efficient carbon and opal flux to the sediments. These records suggest that phytoplankton-mediated changes in the efficiency of the biological carbon pump may govern many accumulations of organic-rich hydrocarbon source rock as well as many abrupt changes in atmospheric pCO[sub 2] and climate.

  14. Geochemical Evidence of Cryptic Sulfur Cycling in Salt Marsh Sediments

    NASA Astrophysics Data System (ADS)

    Mills, J. V.; Antler, G.; Turchyn, A. V.

    2014-12-01

    In modern marine and marginal marine sediments, bacterial sulfate reduction dominates the subsurface oxidation of organic carbon due to the abundance of sulfate in many surface environments. While bacterial sulfate reduction may control anaerobic organic carbon oxidation, there is increasing evidence that iron redox chemistry may be intimately linked to sulfur redox chemistry in the anoxic subsurface, with iron species acting as catalysts or electron shuttles for the microbial use of sulfur, and vice versa. We use stable isotope and geochemical techniques to explore the coupling of the iron and sulfur cycles in salt marsh sediments in North Norfolk, UK. Unique among previously studied environments, these sediments contain high concentrations of both sulfate (20-40mM) and ferrous iron (1-3mM). High ferrous iron concentrations require extended regions of bacterial iron reduction. Within these zones of iron reduction we would predict no sulfate reduction, and lack of change in sulfur isotopes and no loss of sulfate suggest that there is no net sulfate reduction in this zone. However, coincident with the increase in ferrous iron concentrations, the δ18Osulfate exhibits significant increases of up to 5‰. The decoupling of the sulfur and oxygen isotopes of sulfate is suggestive of a cryptic sulfur cycle in which sulfate is reduced to an intermediate valence state sulfur species and subsequently reoxidized to sulfate; this cycle must by quasi-quantitative to produce the suite of geochemical observations. We further explore the nature of this cycling through a series of batch reactor incubation experiments. When sediments are incubated in 18O-enriched water, significant shifts (>15‰) in the δ18Osulfate are observed with no corresponding shift in sulfur isotopes. This provides direct evidence that microbial assemblages in these salt marsh sediments facilitate a cryptic cycling of sulfur, potentially mediated by iron species in the zone of iron reduction. We contrast

  15. Sediment flux modeling: Simulating nitrogen, phosphorus, and silica cycles

    NASA Astrophysics Data System (ADS)

    Testa, Jeremy M.; Brady, Damian C.; Di Toro, Dominic M.; Boynton, Walter R.; Cornwell, Jeffrey C.; Kemp, W. Michael

    2013-10-01

    Sediment-water exchanges of nutrients and oxygen play an important role in the biogeochemistry of shallow coastal environments. Sediments process, store, and release particulate and dissolved forms of carbon and nutrients and sediment-water solute fluxes are significant components of nutrient, carbon, and oxygen cycles. Consequently, sediment biogeochemical models of varying complexity have been developed to understand the processes regulating porewater profiles and sediment-water exchanges. We have calibrated and validated a two-layer sediment biogeochemical model (aerobic and anaerobic) that is suitable for application as a stand-alone tool or coupled to water-column biogeochemical models. We calibrated and tested a stand-alone version of the model against observations of sediment-water flux, porewater concentrations, and process rates at 12 stations in Chesapeake Bay during a 4-17 year period. The model successfully reproduced sediment-water fluxes of ammonium (NH4+), nitrate (NO3-), phosphate (PO43-), and dissolved silica (Si(OH)4 or DSi) for diverse chemical and physical environments. A root mean square error (RMSE)-minimizing optimization routine was used to identify best-fit values for many kinetic parameters. The resulting simulations improved the performance of the model in Chesapeake Bay and revealed (1) the need for an aerobic-layer denitrification formulation to account for NO3- reduction in this zone, (2) regional variability in denitrification that depends on oxygen levels in the overlying water, (3) a regionally-dependent solid-solute PO43- partitioning that accounts for patterns in Fe availability, and (4) a simplified model formulation for DSi, including limited sorption of DSi onto iron oxyhydroxides. This new calibration balances the need for a universal set of parameters that remain true to biogeochemical processes with site-specificity that represents differences in physical conditions. This stand-alone model can be rapidly executed on a

  16. Life cycle assessment for dredged sediment placement strategies.

    PubMed

    Bates, Matthew E; Fox-Lent, Cate; Seymour, Linda; Wender, Ben A; Linkov, Igor

    2015-04-01

    Dredging to maintain navigable waterways is important for supporting trade and economic sustainability. Dredged sediments are removed from the waterways and then must be managed in a way that meets regulatory standards and properly balances management costs and risks. Selection of a best management alternative often results in stakeholder conflict regarding tradeoffs between local environmental impacts associated with less expensive alternatives (e.g., open water placement), more expensive measures that require sediment disposal in constructed facilities far away (e.g., landfills), or beneficial uses that may be perceived as risky (e.g., beach nourishment or island creation). Current sediment-placement decisions often focus on local and immediate environmental effects from the sediment itself, ignoring a variety of distributed and long-term effects from transportation and placement activities. These extended effects have implications for climate change, resource consumption, and environmental and human health, which may be meaningful topics for many stakeholders not currently considered. Life-Cycle Assessment (LCA) provides a systematic and quantitative method for accounting for this wider range of impacts and benefits across all sediment management project stages and time horizons. This paper applies a cradle-to-use LCA to dredged-sediment placement through a comparative analysis of potential upland, open water, and containment-island placement alternatives in the Long Island Sound region of NY/CT. Results suggest that, in cases dealing with uncontaminated sediments, upland placement may be the most environmentally burdensome alternative, per ton-kilometer of placed material, due to the emissions associated with diesel fuel combustion and electricity production and consumption required for the extra handling and transportation. These results can be traded-off with the ecosystem impacts of the sediments themselves in a decision-making framework. PMID:25553545

  17. [High frequency ultrasound].

    PubMed

    Sattler, E

    2015-07-01

    Diagnostic ultrasound has become a standard procedure in clinical dermatology. Devices with intermediate high frequencies of 7.5-15 MHz are used in dermato-oncology for the staging and postoperative care of skin tumor patients and in angiology for improved vessel diagnostics. In contrast, the high frequency ultrasound systems with 20-100 MHz probes offer a much higher resolution, yet with a lower penetration depth of about 1 cm. The main indications are the preoperative measurements of tumor thickness in malignant melanoma and other skin tumors and the assessment of inflammatory and soft tissue diseases, offering information on the course of these dermatoses and allowing therapy monitoring. This article gives an overview on technical principles, devices, mode of examination, influencing factors, interpretation of the images, indications but also limitations of this technique. PMID:25636803

  18. High frequency reference electrode

    DOEpatents

    Kronberg, J.W.

    1994-05-31

    A high frequency reference electrode for electrochemical experiments comprises a mercury-calomel or silver-silver chloride reference electrode with a layer of platinum around it and a layer of a chemically and electrically resistant material such as TEFLON around the platinum covering all but a small ring or halo' at the tip of the reference electrode, adjacent to the active portion of the reference electrode. The voltage output of the platinum layer, which serves as a redox electrode, and that of the reference electrode are coupled by a capacitor or a set of capacitors and the coupled output transmitted to a standard laboratory potentiostat. The platinum may be applied by thermal decomposition to the surface of the reference electrode. The electrode provides superior high-frequency response over conventional electrodes. 4 figs.

  19. High frequency reference electrode

    DOEpatents

    Kronberg, James W.

    1994-01-01

    A high frequency reference electrode for electrochemical experiments comprises a mercury-calomel or silver-silver chloride reference electrode with a layer of platinum around it and a layer of a chemically and electrically resistant material such as TEFLON around the platinum covering all but a small ring or "halo" at the tip of the reference electrode, adjacent to the active portion of the reference electrode. The voltage output of the platinum layer, which serves as a redox electrode, and that of the reference electrode are coupled by a capacitor or a set of capacitors and the coupled output transmitted to a standard laboratory potentiostat. The platinum may be applied by thermal decomposition to the surface of the reference electrode. The electrode provides superior high-frequency response over conventional electrodes.

  20. Molybdenum Cycling in Upwelling Sediments: An Example from Namibian Margin Sediments

    NASA Astrophysics Data System (ADS)

    Arnold, G. L.; Goldhammer, T.; Formolo, M.; Brunner, B.; Ferdelman, T.

    2008-12-01

    The paleo-redox application of molybdenum (Mo) isotopes is strongly tied to our knowledge of the modern marine Mo cycle. Elemental mass balance indicates that ~47% of the Mo supplied to the oceans is removed to deep sea sediments, leaving the remaining Mo to "near-shore" reducing sediments (1). The Black Sea is likely the best studied reducing environment with regards to Mo isotopes, yet accounts for only a small fraction of the Mo mass balance. The accumulation of Mo in continental margin sediments has been recently re-assessed and may account for a larger fraction of the marine Mo reservoir than previously thought (2). In the presence of sulfide, the molybdate anion is transformed, by the replacement of oxygen with sulfur, to particle reactive oxy-thiomolybdates (3). This is often cited as the mechanism by which Mo removal proceeds in the Black Sea where sulfide concentrations in the water are high. In contrast, in continental margin settings, the removal mechanism is poorly understood, and the extent to which sulfur cycling plays a role remains un-quantified. To better understand removal/cycling processes in a continental margin setting, where sulfide may only be present in the pore waters and not in the water column, Mo was studied in an array of marine settings off the Namibian coast. Surface sediments were collected across a transect from near-shore/high productivity to deep water/low productivity sediments. These sediments were incubated in bag experiments to study the relationship between sulfur and Mo cycling. Molybdenum concentrations in the Namibian sediments range from detrital values at the lowest productivity site to 25 ppm in surface sediments with high productivity. Preliminary results allude to a correlation between sulfate reduction rates and Mo accumulation in these sediments. Detailed studies of Mo, Mo isotopes, other trace metals, and sulfur investigations from both sediment cores and bag experiments will be presented. (1)Bertine and Turekian

  1. Working memory impairment in calcineurin knock-out mice is associated with alterations in synaptic vesicle cycling and disruption of high-frequency synaptic and network activity in prefrontal cortex.

    PubMed

    Cottrell, Jeffrey R; Levenson, Jonathan M; Kim, Sung Hyun; Gibson, Helen E; Richardson, Kristen A; Sivula, Michael; Li, Bing; Ashford, Crystle J; Heindl, Karen A; Babcock, Ryan J; Rose, David M; Hempel, Chris M; Wiig, Kjesten A; Laeng, Pascal; Levin, Margaret E; Ryan, Timothy A; Gerber, David J

    2013-07-01

    Working memory is an essential component of higher cognitive function, and its impairment is a core symptom of multiple CNS disorders, including schizophrenia. Neuronal mechanisms supporting working memory under normal conditions have been described and include persistent, high-frequency activity of prefrontal cortical neurons. However, little is known about the molecular and cellular basis of working memory dysfunction in the context of neuropsychiatric disorders. To elucidate synaptic and neuronal mechanisms of working memory dysfunction, we have performed a comprehensive analysis of a mouse model of schizophrenia, the forebrain-specific calcineurin knock-out mouse. Biochemical analyses of cortical tissue from these mice revealed a pronounced hyperphosphorylation of synaptic vesicle cycling proteins known to be necessary for high-frequency synaptic transmission. Examination of the synaptic vesicle cycle in calcineurin-deficient neurons demonstrated an impairment of vesicle release enhancement during periods of intense stimulation. Moreover, brain slice and in vivo electrophysiological analyses showed that loss of calcineurin leads to a gene dose-dependent disruption of high-frequency synaptic transmission and network activity in the PFC, correlating with selective working memory impairment. Finally, we showed that levels of dynamin I, a key presynaptic protein and calcineurin substrate, are significantly reduced in prefrontal cortical samples from schizophrenia patients, extending the disease relevance of our findings. Our data provide support for a model in which impaired synaptic vesicle cycling represents a critical node for disease pathologies underlying the cognitive deficits in schizophrenia. PMID:23825400

  2. Manganese, iron, and sulfur cycling in Louisiana continental shelf sediments

    NASA Astrophysics Data System (ADS)

    Devereux, Richard; Lehrter, John C.; Beddick, David L.; Yates, Diane F.; Jarvis, Brandon M.

    2015-05-01

    Sulfate reduction is considered the primary pathway for organic carbon remineralization on the northern Gulf of Mexico Louisiana continental shelf (LCS) where bottom waters are seasonally hypoxic, yet limited information is available on the importance of iron and manganese cycling in the region. Sedimentary manganese, iron, and sulfur cycling were investigated on the LCS using a combined chemical analysis and sediment diagenesis modeling approach. Three stations situated 320 km across the LCS along the 20 m isobath were sampled up to five times between the spring of 2006 and summer of 2007. Bottom water oxygen levels at the stations ranged from 203 mmol m-3 in spring to 2.5 mmol m-3 in summer. Porewater Mn and Fe2+ concentrations (up to 275 and 300 μmol L-1, respectively), sulfate reduction rates (1.0-8.4 mmol m-2 d-1), and the fraction of total oxalate extracted iron obtained as Fe(II) (0.25-0.52) differed between station and season. Sediments at station Z02 on the eastern LCS, south of Terrebonne Bay, had higher organic matter content and sulfate reduction rates than sediments at Z03, 160 km further west. Sulfate reduction rates were higher in summer than spring at station Z02 but not at Z03 where porewater Mn and Fe concentrations were highest in summer. Porewater Fe2+ concentrations, solid phase oxalate-extractable Fe concentrations, and sediment incubation experiments suggested iron reduction at Z03 may account for 20% or more of organic carbon remineralization. LCS Fe(III) concentrations decreased and sulfate reduction rates increased in model simulations by lowering interfacial dissolved oxygen levels and increasing the rates of organic matter deposited on the sediment surface. Results from this study demonstrate that LCS sedimentary metal oxide cycling may be more important in organic carbon mineralization pathways than previously recognized.

  3. Arctic ocean sediment texture and the Pleistocene climate cycle

    SciTech Connect

    Clark, D.L.; Morris, T.H.

    1985-01-01

    Arctic Ocean sediment texture accurately reflects the Plio-Pleistocene climate cycle. The precision of paleoclimate interpretation is improved when deglaciation is recognized as a distinct climate stage, overlapping both glacial and interglacial stages, and for the later Pleistocene, perhaps never completed. Oxygen isotope stratigraphy and foraminifera productivity are out of phase but can be understood in the context of the transitional nature of the glacial, deglacial and interglacial climate stages of the Arctic Ocean.

  4. Ni cycling in mangrove sediments from New Caledonia

    NASA Astrophysics Data System (ADS)

    Noël, Vincent; Morin, Guillaume; Juillot, Farid; Marchand, Cyril; Brest, Jessica; Bargar, John R.; Muñoz, Manuel; Marakovic, Grégory; Ardo, Sandy; Brown, Gordon E.

    2015-11-01

    sediment layers. Ni-incorporation in pyrite is especially observed beneath an inland Avicennia stand where anoxic conditions are dominant. In contrast, beneath a Rhizophora stand closer to the ocean, where the redox cycle is intensified due to the tide cycle, partial re-oxidation of Ni-bearing pyrites favors nickel mobility, as confirmed by Ni-mass balance estimates and by higher Ni concentration in the pore waters. These findings have important environmental implications for better evaluating the protective role of mangroves against trace metal dispersion into marine ecosystems. They may also help in predicting the response of mangrove ecosystems to increasing anthropogenic pressure on coastal areas.

  5. High frequency electromagnetic tomography

    SciTech Connect

    Daily, W.; Ramirez, A.; Ueng, T.; Latorre, R.

    1989-09-01

    An experiment was conducted in G Tunnel at the Nevada Test Site to evaluate high frequency electromagnetic tomography as a candidate for in situ monitoring of hydrology in the near field of a heater placed in densely welded tuff. Tomographs of 200 MHz electromagnetic permittivity were made for several planes between boreholes. Data were taken before the heater was turned on, during heating and during cooldown of the rockmass. This data is interpreted to yield maps of changes in water content of the rockmass as a function of time. This interpretation is based on laboratory measurement of electromagnetic permittivity as a function of water content for densely welded tuff. 8 refs., 6 figs.

  6. High-frequency ventilation.

    PubMed

    Crawford, M R

    1986-08-01

    Over the last six years high-frequency ventilation has been extensively evaluated both in the clinical and laboratory settings. It is now no longer the great mystery it once was, and it is now no longer believed (as many had hoped), that it will solve all the problems associated with mechanical pulmonary ventilation. Although the technique is safe and appears to cause no harm even in the long term, it has not yet been shown to offer any major advantages over conventional mechanical ventilation. PMID:3530042

  7. Temporal variability of exchange between groundwater and surface water based on high-frequency direct measurements of seepage at the sediment-water interface

    NASA Astrophysics Data System (ADS)

    Rosenberry, Donald O.; Sheibley, Richard W.; Cox, Stephen E.; Simonds, Frederic W.; Naftz, David L.

    2013-05-01

    Seepage at the sediment-water interface in several lakes, a large river, and an estuary exhibits substantial temporal variability when measured with temporal resolution of 1 min or less. Already substantial seepage rates changed by 7% and 16% in response to relatively small rain events at two lakes in the northeastern USA, but did not change in response to two larger rain events at a lake in Minnesota. However, seepage at that same Minnesota lake changed by 10% each day in response to withdrawals from evapotranspiration. Seepage increased by more than an order of magnitude when a seiche occurred in the Great Salt Lake, Utah. Near the head of a fjord in Puget Sound, Washington, seepage in the intertidal zone varied greatly from -115 to +217 cm d-1 in response to advancing and retreating tides when the time-averaged seepage was upward at +43 cm d-1. At all locations, seepage variability increased by one to several orders of magnitude in response to wind and associated waves. Net seepage remained unchanged by wind unless wind also induced a lake seiche. These examples from sites distributed across a broad geographic region indicate that temporal variability in seepage in response to common hydrological events is much larger than previously realized. At most locations, seepage responded within minutes to changes in surface-water stage and within minutes to hours to groundwater recharge associated with rainfall. Likely implications of this dynamism include effects on water residence time, geochemical transformations, and ecological conditions at and near the sediment-water interface.

  8. σ2-Adaptin Facilitates Basal Synaptic Transmission and Is Required for Regenerating Endo-Exo Cycling Pool Under High-Frequency Nerve Stimulation in Drosophila.

    PubMed

    Choudhury, Saumitra Dey; Mushtaq, Zeeshan; Reddy-Alla, Suneel; Balakrishnan, Sruthi S; Thakur, Rajan S; Krishnan, Kozhalmannom S; Raghu, Padinjat; Ramaswami, Mani; Kumar, Vimlesh

    2016-05-01

    The functional requirement of adapter protein 2 (AP2) complex in synaptic membrane retrieval by clathrin-mediated endocytosis is not fully understood. Here we isolated and functionally characterized a mutation that dramatically altered synaptic development. Based on the aberrant neuromuscular junction (NMJ) synapse, we named this mutation angur (a Hindi word meaning "grapes"). Loss-of-function alleles of angur show more than twofold overgrowth in bouton numbers and a dramatic decrease in bouton size. We mapped the angur mutation to σ2-adaptin, the smallest subunit of the AP2 complex. Reducing the neuronal level of any of the subunits of the AP2 complex or disrupting AP2 complex assembly in neurons phenocopied the σ2-adaptin mutation. Genetic perturbation of σ2-adaptin in neurons leads to a reversible temperature-sensitive paralysis at 38°. Electrophysiological analysis of the mutants revealed reduced evoked junction potentials and quantal content. Interestingly, high-frequency nerve stimulation caused prolonged synaptic fatigue at the NMJs. The synaptic levels of subunits of the AP2 complex and clathrin, but not other endocytic proteins, were reduced in the mutants. Moreover, bone morphogenetic protein (BMP)/transforming growth factor β (TGFβ) signaling was altered in these mutants and was restored by normalizing σ2-adaptin in neurons. Thus, our data suggest that (1) while σ2-adaptin facilitates synaptic vesicle (SV) recycling for basal synaptic transmission, its activity is also required for regenerating SVs during high-frequency nerve stimulation, and (2) σ2-adaptin regulates NMJ morphology by attenuating TGFβ signaling. PMID:26920756

  9. Tempo and scale of biogenic effects on high-frequency acoustic propagation near the marine sediment-water interface in shallow water

    NASA Astrophysics Data System (ADS)

    Jumars, Peter

    2003-04-01

    Organisms have natural scales, such as lifetimes, body sizes, frequencies of movement to new locations, and residence times of material in digestive systems, and each scale has potential implications for acoustic effects. The effects of groups of organisms, like organisms themselves, aggregate in space and time. This review, including an assortment of unpublished information, examines examples of such aggregations, many of them documented acoustically. Light synchronizes many activities. Macroscopic animals forage primarily under cover of darkness. This phasing applies both to animals that extend appendages above the sediment-water interface and to animals that leave the seabed at night. Whereas their bottom-modifying activities are concentrated in nocturnal or crepuscular fashion, the bottom-modifying activities of the visual feeders follow a different phasing and often dominate the rate of change in acoustic backscatter from the interface. Light also acts through its effects on primary production, often concentrated in a very thin surficial layer atop the seabed. The supersaturation of oxygen does, and microbubble nucleation may, result. Where tidal velocities are large, light-set patterns are often tidally modulated. Activities of animals living below the seabed, however, remain a mystery, whose primary hope for solution is acoustic. [Work supported by ONR and DEPSCoR.

  10. Linking soil and sediment properties for research on biogeochemical cycles

    NASA Astrophysics Data System (ADS)

    Kuhn, Nikolaus J.

    2013-04-01

    Conventional perspectives on soil erosion include the on-site damage to soil and reductions in crop yield, as well as the resulting off-site effects on water quality, runoff and sediment loads in rivers. Our evolving understanding of the Earth System has added a new dimension to the role of soil erosion within the global geochemical cycles. First, the relevance of soil as a nutrient and Carbon (C) pool was recognized. Initially, the role of soils in the global C cycle was largely considered to be limited to a vertical exchange of greenhouse house gases (GHG) between vegetation, soil and atmosphere and thus mostly studied by soil scientists, plant ecologists and climatologists. Even Critical Zone research focused mostly on weathering and regolith properties and ignored lateral fluxes of dissolved or particulate organic matter. Since the late 1990s, a wider role of soils in biogeochemical cycles has emerged. Recent estimates place the lateral movement of C between soil and sediment pools in terrestrial ecosystems (including rivers and lakes) at approximately 0.6 to 1.5 Gt per year. Some of the eroded C is replaced by photosynthesis from the atmosphere, but at a cost of additional emissions, for example due to fertilizer production. The long-term fate of the eroded and deposited soil organic matter is subject to an open debate and suffers from a lack of reliable spatial information on lateral C fluxes and its subsequent fate in terrestrial ecosystems. The connection between soil C pool, GHG emissions and erosion illustrates the relevance of surface processes for the C fluxes between Earth's spheres. Accordingly, soil is now considered as mobile system to make accurate predictions about the consequences of global change for terrestrial biogeochemical cycles and climate feedbacks. This expanded perspective on soils as dynamic pool of weathering regolith, sediment, nutrients and C at the interface between the geospheres requires the analysis of relevant soil properties

  11. Linking Soil and Sediment Properties for research on Biogeochemical Cycles

    NASA Astrophysics Data System (ADS)

    Kuhn, N. J.

    2012-04-01

    Conventional perspectives on soil erosion include the on-site damage to soil and reductions in crop yield, as well as the resulting off-site effects on water quality, runoff and sediment loads in rivers. Our evolving understanding of the Earth System has added a new dimension to the role of soil erosion within the global geochemical cycles. First, the relevance of soil as a nutrient and Carbon (C) pool was recognized. Initially, the role of soils in the global C cycle was largely considered to be limited to a vertical exchange of greenhouse house gases (GHG) between vegetation, soil and atmosphere and thus mostly studied by soil scientists, plant ecologists and climatologists. Even Critical Zone research focused mostly on weathering and regolith properties and ignored lateral fluxes of dissolved or particulate organic matter. Since the late 1990s, a wider role of soils in biogeochemical cycles has emerged. Recent estimates place the lateral movement of C between soil and sediment pools in terrestrial ecosystems (including rivers and lakes) at approximately 0.6 to 1.5 Gt per year. Some of the eroded C is replaced by photosynthesis from the atmosphere, but at a cost of additional emissions, for example due to fertilizer production. The long-term fate of the eroded and deposited soil organic matter is subject to an open debate and suffers from a lack of reliable spatial information on lateral C fluxes and its subsequent fate in terrestrial ecosystems. The connection between soil C pool, GHG emissions and erosion illustrates the relevance of surface processes for the C fluxes between Earth's spheres. Accordingly, soil is now considered as mobile system to make accurate predictions about the consequences of global change for terrestrial biogeochemical cycles and climate feedbacks. This expanded perspective on soils as dynamic pool of weathering regolith, sediment, nutrients and C at the interface between the geospheres requires the analysis of relevant soil properties

  12. Resource quality affects carbon cycling in deep-sea sediments.

    PubMed

    Mayor, Daniel J; Thornton, Barry; Hay, Steve; Zuur, Alain F; Nicol, Graeme W; McWilliam, Jenna M; Witte, Ursula F M

    2012-09-01

    Deep-sea sediments cover ~70% of Earth's surface and represent the largest interface between the biological and geological cycles of carbon. Diatoms and zooplankton faecal pellets naturally transport organic material from the upper ocean down to the deep seabed, but how these qualitatively different substrates affect the fate of carbon in this permanently cold environment remains unknown. We added equal quantities of (13)C-labelled diatoms and faecal pellets to a cold water (-0.7 °C) sediment community retrieved from 1080 m in the Faroe-Shetland Channel, Northeast Atlantic, and quantified carbon mineralization and uptake by the resident bacteria and macrofauna over a 6-day period. High-quality, diatom-derived carbon was mineralized >300% faster than that from low-quality faecal pellets, demonstrating that qualitative differences in organic matter drive major changes in the residence time of carbon at the deep seabed. Benthic bacteria dominated biological carbon processing in our experiments, yet showed no evidence of resource quality-limited growth; they displayed lower growth efficiencies when respiring diatoms. These effects were consistent in contrasting months. We contend that respiration and growth in the resident sediment microbial communities were substrate and temperature limited, respectively. Our study has important implications for how future changes in the biochemical makeup of exported organic matter will affect the balance between mineralization and sequestration of organic carbon in the largest ecosystem on Earth. PMID:22378534

  13. Anaerobic Redox Cycling of Iron by Freshwater Sediment Microorganisms

    SciTech Connect

    Weber, Karrie A.; Urrutia, Matilde M.; Churchill, Perry F.; Kukkadapu, Ravi K.; Roden, Eric E.

    2006-01-01

    The potential for microbially-mediated anaerobic redox cycling of iron (Fe) was examined in a first-generation enrichment culture of freshwater wetland sediment microorganisms. MPN enumerations revealed the presence of significant populations of Fe(III)-reducing (ca. 108 cells mL-1) and Fe(II)-oxidizing, nitrate-reducing organisms (ca. 105 cells mL-1) in the sediment used to inoculate the enrichment cultures. Nitrate reduction commenced immediately following inoculation of acetate-containing (ca. 1 mM) medium with a small quantity (1% vol/vol) of wetland sediment, and resulted in the transient accumulation of NO2- and production of a mixture of end-products including NH4+. Fe(III) oxide (high surface area goethite) reduction took place - after NO3- was depleted and continued until all the acetate was utilized. Addition of NO3 after Fe(III) reduction ceased resulted in the immediate oxidation of Fe(II) coupled to reduction of + NO3-to NH4 . No significant NO2- accumulation was observed during nitrate-dependent Fe(II) oxidation. No Fe(II) oxidation occurred in pasteurized controls. Microbial community structure in the enrichment was monitored by DGGE analysis of PCR amplified 16s rDNA and RT-PCR amplified 16S rRNA, as well as by construction of 16S rDNA clone libraries for four different time points during the experiment. Strong similarities in dominant members of the microbial community were observed in the Fe(III) reduction and nitrate-dependent Fe(II) oxidation phases of the experiment, specifically the common presence of organisms closely related (= 95% sequence similarity) to the genera Geobacter and Dechloromonas. These results indicate that the wetland sediments contained organisms such as Geobacter sp. which are capable of both + dissimilatory Fe(III) reduction and oxidation of Fe(II) with reduction of NO3-reduction to NH4 . Our findings suggest that microbially-catalyzed nitrate-dependent Fe(II) oxidation has the potential to contribute to a dynamic

  14. High-frequency ECG

    NASA Technical Reports Server (NTRS)

    Tragardh, Elin; Schlegel, Todd T.

    2006-01-01

    The standard ECG is by convention limited to 0.05-150 Hz, but higher frequencies are also present in the ECG signal. With high-resolution technology, it is possible to record and analyze these higher frequencies. The highest amplitudes of the high-frequency components are found within the QRS complex. In past years, the term "high frequency", "high fidelity", and "wideband electrocardiography" have been used by several investigators to refer to the process of recording ECGs with an extended bandwidth of up to 1000 Hz. Several investigators have tried to analyze HF-QRS with the hope that additional features seen in the QRS complex would provide information enhancing the diagnostic value of the ECG. The development of computerized ECG-recording devices that made it possible to record ECG signals with high resolution in both time and amplitude, as well as better possibilities to store and process the signals digitally, offered new methods for analysis. Different techniques to extract the HF-QRS have been described. Several bandwidths and filter types have been applied for the extraction as well as different signal-averaging techniques for noise reduction. There is no standard method for acquiring and quantifying HF-QRS. The physiological mechanisms underlying HF-QRS are still not fully understood. One theory is that HF-QRS are related to the conduction velocity and the fragmentation of the depolarization wave in the myocardium. In a three-dimensional model of the ventricles with a fractal conduction system it was shown that high numbers of splitting branches are associated with HF-QRS. In this experiment, it was also shown that the changes seen in HF-QRS in patients with myocardial ischemia might be due to the slowing of the conduction velocity in the region of ischemia. This mechanism has been tested by Watanabe et al by infusing sodium channel blockers into the left anterior descending artery in dogs. In their study, 60 unipolar ECGs were recorded from the entire

  15. Characterizing seasonal variability of storm events based on very high frequency monitoring of hydrological and chemical variables: comparing patterns in hot spots and hot moments for nutrient and sediment export

    NASA Astrophysics Data System (ADS)

    Fovet, Ophelie; Thelusma, Gilbert; Humbert, Guillaume; Dupas, Rémi; Faucheux, Mikael; Gilliet, Nicolas; Hamon, Yannick; Jaffrezic, Anne; Grimaldi, Catherine; Gruau, Gerard

    2016-04-01

    Storm events are critical hot moments of emission for several dissolved and particulate chemical species at major stake for water quality (e.g. dissolved organic carbon DOC, suspended sediments, phosphorus). During such events, the solutes or particles are exported from heterogeneous sources through various pathways to stream leading to specific integrated signals at the outlet characterized by very short dynamics. This is merely true in headwater catchments where the total duration of such events ranges over 10h to 3 days, with very quick variations in stream flow and concentrations at the outlet occurring in a few hours. Thus for investigating properly event processes, high frequency monitoring of flow and water quality is required. We analysed 103 storm events in a 5 km2 agricultural headwater catchment, part of the AgrHys Observatory, on the basis of a 3-year-long data set which combined meterological (Rainfall), hydrological (flow and piezometry), and water quality (turbidity, conductivity, DOC and NO3 concentrations) data recorded at very high frequencies (from 1 to 20 min) thanks to dedicated sensors. We proposed a range of quantitative storm descriptors for characterizing input (rainfall), antecedent and initial conditions (groundwater levels and saturated area), and stream response in terms of level and dynamics of flow (Q), groundwater levels, and concentrations (C) but also the C-Q relationships. Three intra annual periods have been previously defined for base flow dynamic according to shallow groundwater table variations so that they correspond to different connectivity status in the catchment. The seasonal and inter-annual variability of the storm events have been analysed using the descriptors and based on these predefined periods. Results show that the hydrological flowpaths and the consequent storm chemistry were controlled by the hydrological base flow regime rather than by the rain input characteristics. This highlights that the exports of NO3

  16. Carbon and nitrogen cycling in thermally heated sediments

    NASA Astrophysics Data System (ADS)

    Meyer-Dombard, D. R.; Burton, M.; Vennelakanti, S.; Havig, J. R.; Shock, E.

    2009-12-01

    Hydrothermally heated sediment environments, such as are found in abundance throughout Yellowstone National Park, host fully functional microbial ecosystems. As with any ecosystem, both sources and sinks of carbon, nitrogen, and a myriad of other nutrients and energy-driving factors must be supplied. While we know microbial communities in hydrothermal environments can be surprisingly diverse, we know little about basic ecological functions such as carbon and nitrogen cycling. Previous work has shown that carbon cycling in one hot spring in Yellowstone National Park [“Bison Pool”] and its associated runoff channel functions as a complex system. Analysis of carbon and nitrogen isotopes in sediments and biofilms across a temperature and chemical gradient at this location revealed that the four best studied carbon fixation pathways [Calvin, reverse tricarboxylic acid, acetyl-CoA, 3-hydroxypropionate cycles] may all be functioning in this system, and nitrogen fixation varies across the chemosynthetic/photosynthetic ecotone [1]. Microcosm experiments using biofilms from this hot spring as inoculae with 13C labeled carbon substrates indicate heterotrophic growth [2]. In addition, metagenomic analysis of environmental DNA has indicated the presence of genes involved in carbon fixation [both phototrophic and autotrophic], and heterotrophy, as well as nitrogen fixation [3]. Studies from other Yellowstone locations have also found genetic evidence for carbon and nitrogen fixation [4, 5]. Of particular interest is the role of individuals in carbon and nitrogen cycling as environmental conditions suitable for chemosynthetic and photosynthetic growth vary. This study explores the diversity of cbbM/cbbL [Calvin cycle], aclB/oor/porA [rTCA cycle], nifH [nitrogen fixation], nirK [nitrite reduction] and amoA [ammonia oxidation] genes across a variety of Yellowstone environments. The transition of genetic diversity within sediments and biofilms is focused on the chemosynthetic

  17. Nitrogen cycling in Hot Spring Sediments and Biofilms (Invited)

    NASA Astrophysics Data System (ADS)

    Meyer-Dombard, D. R.; Burton, M. S.; Havig, J. R.; Shock, E.

    2010-12-01

    Over the past several decades, gene-targeted analyses have revealed that microbial communities in hydrothermal environments can be surprisingly diverse. However, we know shockingly little about basic ecological functions such as carbon and nitrogen cycling or community shifts over time, or environmental parameters such as growth criteria. Previous work has shown that carbon cycling in one hot spring in Yellowstone National Park [“Bison Pool”] and its associated runoff channel functions as a complex system. Analysis of carbon and nitrogen isotopes in biofilms across a temperature and chemical gradient at this location revealed that multiple autotrophic carbon fixation pathways are functioning in this system, and nitrogen fixation varies across the chemosynthetic/photosynthetic ecotone [1]. Further, sequencing of metagenomes from multiple locations at “Bison Pool” has indicated the presence of genes involved in carbon fixation [both phototrophic and autotrophic], and heterotrophy, as well as nitrogen fixation [2]. Studies from other Yellowstone locations have also found genetic evidence for carbon and nitrogen fixation [3-5]. The role of individual microbes in nitrogen cycling as environmental conditions vary over space and time is the focus of this study. Here, we explore the diversity of nifH [nitrogen fixation], nirK [nitrite reduction] and amoA [ammonia oxidation] genes across a variety of Yellowstone environments. Environmental nucleic acids were extracted, and the presence/absence of Bacteria and Archaea determined by PCR. In addition, PCR-directed screens reveal the presence or absence of the aforementioned functional genes, indicating genetic capacity for nitrogen cycling. We have examined the transition of genetic diversity and genetic capacity within sediments and biofilms at the chemosynthetic/photosynthetic ecotone in several hot springs spanning ranges of pH and geochemical conditions. By sampling across this ecotone, changes in the genetic

  18. Nitrogen cycling in different types of sediments from Danish waters

    SciTech Connect

    Blackburn, T.H.; Henridsen, K.

    1983-05-01

    Variations in sediment N:C ratios were correlated with water depth and season. /sup 14/NH/sub 4//sup +/ was used to measure the rates of NH/sub 4//sup +/ production (d) and incorporation into bacterial cells (i) in sediments from different stations, at different seasons. The validity of the rates d and i was indicated by the predicted correlation of d:i ratios with N:C ratios of the sediment, and the predicted N:C ratio at which net NH/sub 4//sup +/; pore water NH/sub 4//sup +/, flux of NH/sub 4//sup +/ from sediment, and flux of NH/sub 4//sup +/ into exchangeable pool. The NO/sub 3//sup -/ flux from sediment was correlated with nitrification rate and with season. Benthic infauna increased the flux of NH/sub 4//sup +/ from the sediment by 50%. The rates of transfer of nitrogen (NO/sub 3//sup -/, NH/sub 4//sup +/, N/sub 2/) from sediment to water were 44-66% of the net rates of organic nitrogen mineralization (d-i). Flux of NO/sub 3//sup -/ + NH/sub 4//sup +/ from the sediment could supply 30-82% of the nitrogen requirement of the planktonic primary producers.

  19. Influence of the submersed plant, Potamogeton perfoliatus, on nitrogen cycling in estuarine sediments

    USGS Publications Warehouse

    Caffrey, J.M.; Kemp, W.M.

    1993-01-01

    Using 15N isotope techniques P. perfoliatus is shown to have a significant influence on sediment N cycling by direct uptake of NH4+ and NO3- and by indirect mechanisms leading to enhanced nitrification and denitrification. -from Authors

  20. Biogeochemical Cycle of Methanol in Anoxic Deep-Sea Sediments

    PubMed Central

    Yanagawa, Katsunori; Tani, Atsushi; Yamamoto, Naoya; Hachikubo, Akihiro; Kano, Akihiro; Matsumoto, Ryo; Suzuki, Yohey

    2016-01-01

    The biological flux and lifetime of methanol in anoxic marine sediments are largely unknown. We herein reported, for the first time, quantitative methanol removal rates in subsurface sediments. Anaerobic incubation experiments with radiotracers showed high rates of microbial methanol consumption. Notably, methanol oxidation to CO2 surpassed methanol assimilation and methanogenesis from CO2/H2 and methanol. Nevertheless, a significant decrease in methanol was not observed after the incubation, and this was attributed to the microbial production of methanol in parallel with its consumption. These results suggest that microbial reactions play an important role in the sources and sinks of methanol in subseafloor sediments. PMID:27301420

  1. High-Frequency Gated Oscillator

    NASA Technical Reports Server (NTRS)

    Berard, C. A.

    1982-01-01

    New gated oscillator generates bursts of high-frequency sine waves, square waves, and triangular waves in response to control signals. Each burst starts at zero phase, with tight tolerances on signal amplitude and frequency. Frequencies in megahertz range are made possible by using high-speed comparators and high-speed flip-flop as fast-response threshold detector.

  2. High frequency integrated MOS filters

    NASA Technical Reports Server (NTRS)

    Peterson, C.

    1990-01-01

    Several techniques exist for implementing integrated MOS filters. These techniques fit into the general categories of sampled and tuned continuous-time filters. Advantages and limitations of each approach are discussed. This paper focuses primarily on the high frequency capabilities of MOS integrated filters.

  3. The carbon cycle and biogeochemical dynamics in lake sediments

    USGS Publications Warehouse

    Dean, W.E.

    1999-01-01

    The concentrations of organic carbon (OC) and CaCO3 in lake sediments are often inversely related. This relation occurs in surface sediments from different locations in the same lake, surface sediments from different lakes, and with depth in Holocene sediments. Where data on accumulation rates are available, the relation holds for organic carbon and CaCO3 accumulation rates as well. An increase of several percent OC is accompanied by a decrease of several tens of percent CaCO3 indicating that the inverse relation is not due to simple dilution of one component by another. It appears from core data that once the OC concentration in the sediments becomes greater than about 12%, the CO2 produced by decomposition of that OC and production of organic acids lowers the pH of anoxic pore waters enough to dissolve any CaCO3 that reaches the sediment-water interface. In a lake with a seasonally anoxic hypolimnion, processes in the water column also can produce an inverse relation between OC and CaCO3 over time. If productivity of the lake increases, the rain rate of OC from the epilimnion increases. Biogenic removal of CO2 and accompanying increase in pH also may increase the production of CaCO3. However, the decomposition of organic matter in the hypolimnion will decrease the pH of the hypolimnion causing greater dissolution of CaCO3 and therefore a decrease in the rain rate of CaCO3 to the sediment-water interface.

  4. The impact of electrogenic sulfide oxidation on elemental cycling and solute fluxes in coastal sediment

    NASA Astrophysics Data System (ADS)

    Rao, Alexandra M. F.; Malkin, Sairah Y.; Hidalgo-Martinez, Silvia; Meysman, Filip J. R.

    2016-01-01

    Filamentous sulfide oxidizing cable bacteria are capable of linking the oxidation of free sulfide in deep anoxic layers of marine sediments to the reduction of oxygen or nitrate in surface sediments by conducting electrons over centimeter-scale distances. Previous studies have shown that this newly discovered microbial process, referred to as electrogenic sulfide oxidation (e-SOx), may alter elemental cycling in sediments, but the nature and rates of the resulting biogeochemical transformations and their influence on benthic-pelagic coupling remain largely unknown. Here we quantify changes in sediment geochemistry and solute fluxes at the sediment-water interface as e-SOx develops and declines over time in laboratory incubations of organic-rich sediments from a seasonally hypoxic coastal basin (Marine Lake Grevelingen, The Netherlands). Our results show that e-SOx enhanced sediment O2 consumption and acidified subsurface sediment, resulting in the dissolution of calcium carbonate and iron sulfide minerals in deeper sediment horizons and the associated accumulation of dissolved iron, manganese, and calcium in porewater. Remobilized Fe diffusing upward was reoxidized at the sediment-water interface, producing an amorphous Fe oxide crust, while dissolved Fe diffusing downward was reprecipitated in the form of FeS as it encountered the free sulfide horizon. The development of e-SOx enhanced the diffusive release of dissolved Mn at the sediment-water interface, capped the phosphate efflux, generated a buildup of organic matter in surface sediments, and strongly stimulated the release of alkalinity from the sediment. About 75% of this alkalinity production was associated with net CaCO3 dissolution, while the remaining 25% was attributed to a pumping mechanism that transfers alkalinity from anodic H2S oxidation (an alkalinity sink) in deeper sediments to cathodic O2 reduction (an alkalinity source) near the sediment-water interface. The resulting sediment alkalinity

  5. Viral activities and life cycles in deep subseafloor sediments.

    PubMed

    Engelhardt, Tim; Orsi, William D; Jørgensen, Bo Barker

    2015-12-01

    Viruses are highly abundant in marine subsurface sediments and can even exceed the number of prokaryotes. However, their activity and quantitative impact on microbial populations are still poorly understood. Here, we use gene expression data from published continental margin subseafloor metatranscriptomes to qualitatively assess viral diversity and activity in sediments up to 159 metres below seafloor (mbsf). Mining of the metatranscriptomic data revealed 4651 representative viral homologues (RVHs), representing 2.2% of all metatranscriptome sequence reads, which have close translated homology (average 77%, range 60-97% amino acid identity) to viral proteins. Archaea-infecting RVHs are exclusively detected in the upper 30 mbsf, whereas RVHs for filamentous inoviruses predominate in the deepest sediment layers. RVHs indicative of lysogenic phage-host interactions and lytic activity, notably cell lysis, are detected at all analysed depths and suggest a dynamic virus-host association in the marine deep biosphere studied here. Ongoing lytic viral activity is further indicated by the expression of clustered, regularly interspaced, short palindromic repeat-associated cascade genes involved in cellular defence against viral attacks. The data indicate the activity of viruses in subsurface sediment of the Peruvian margin and suggest that viruses indeed cause cell mortality and may play an important role in the turnover of subseafloor microbial biomass. PMID:26109514

  6. [Geochemical cycling of mercury in the sediment of Hongfeng Reservior].

    PubMed

    He, Tian-Rong; Feng, Xin-Bin; Guo, Yan-Na; Meng, Bo; Li, Zhong-Gen; Qiu, Guang-Le; Liang, Lian

    2008-07-01

    Spatial and temporal distributions of total and methyl mercury and controlling factors were investigated based on cold vapor atomic fluorescence detection. Total mercury levels in the whole sediments are (0.392 +/- 0.070) microg/g, without significant variations between different seasons, but generally increase toward the sediment-water interface. Total mercury levels are higher compared to data reported in other uncontaminated reservoirs and Wujiangdu Reservoir. This indicates there are mercury contaminations in Hongfeng Reservoir. Methyl mercury concentrations are highest in spring, without significant variations in other seasons. The peak values of methyl mercury typically appear in the upper 8 cm of the sediment profiles which are also the zones of sulfate-reducing bacteria activities. The seasonal variation and maximum peak value distributions of methyl mercury in sediment are mainly controlled by seasonally migration of oxic/anoxic boundary layer. Total mercury concentrations in the pore water and partition coefficients for THg in solid phase and water phase are mainly controlled by temperature or redox potential. Total mercury concentrations in the pore water have no relationship with total mercury concentrations in solid phase. However, the methyl mercury concentrations in the pore water have a strong relationship with those in solid phase (r = 0.70, p < 0.001). The methyl mercury concentrations in solid phase and pore water are controlled by solid/water partition coefficient, as well as methyl mercury production. PMID:18828352

  7. Enzymatic assays of sediments from North Pond (IODP Expedition 336) to elucidate microbial phosphorus cycling strategies

    NASA Astrophysics Data System (ADS)

    Defforey, D.; Paytan, A.

    2015-12-01

    Phosphorus (P) is a key macronutrient for living cells and its availability is limited in the deep subseafloor environment, a habitat estimated to contain up to 1% of Earth's total biomass. The existence and activity of deep subseafloor microbial populations have profound implications on global biogeochemical cycles and our understanding of the limits of life. However, little is known about the impact of the deep biosphere on sedimentary P cycling and P diagenetic processes. Our previous work has shown that sedimentary P at North Pond is mainly present in mineral phases, and that refractory organic P is detectable throughout the sediment column. The latter could constitute a P source to the deep biosphere. Alternatively, microorganisms could have mechanisms to harvest P from recalcitrant mineral phases. The aim of this study is to determine the presence and maximum potential activity of enzymes involved in microbial P uptake in deep-sea sediments. These include phosphomonoesterases, such as alkaline phosphatase, phosphodiesterases, pyrophosphatase and phosphonatases. The sediment samples used for this study were collected at North Pond, a sediment pond located on the western flank of the Mid-Atlantic Ridge, during IODP Expedition 336. This work will provide key insights into the microbial P uptake mechanisms at play in open ocean sediments, and their effects on sedimentary P cycling. These results, in conjunction with our previous work investigating P geochemistry at North Pond, will yield valuable information regarding the impact of the deep biosphere on P cycling in open ocean sediments.

  8. The Effect of Sediment Disturbance on the Carbon and Nitrogen Cycles Within Coastal Marine Lakes

    NASA Astrophysics Data System (ADS)

    Mills, A. R.; Wilson, S. R.; Jolley, D. F.

    2006-12-01

    A system allowing the ex situ quantification of the nitrogen cycle and various carbonaceous species (Organic Carbon, carbon dioxide, methane) in the air, water and sediment has been developed and used to monitor the effects of sediment disturbance on the nitrogen and carbon cycles. Measurements are made using standard wet chemical techniques for the sediment and water phases, and FTIR spectroscopy for the real-time monitoring of gas concentrations. Sediment collected from two temperate marine lakes on the eastern coast of Australia has been examined. In the laboratory it was found that the methane flux peaked three days after the initial disturbance; nitrous oxide flux peaked after 12 days while carbon dioxide flux varied throughout the experiment. Nitrite and nitrate concentrations in the overlying water peaked at the end of the second and third weeks, respectively. The total quantity of nitrogen within the system increased by ~ 35%. Sediment disturbance led to an initial release of nitrogenous nutrients from the sediment to the overlying water that were rapidly readsorbed to sediment particles and sequestered back into the sediment phase. The same technology was also adapted to allow this research to be undertaken in the field and used to study three new temperate marine sites within Lake Illawarra, New South Wales, Australia.

  9. Mobile high frequency vibrator system

    SciTech Connect

    Fair, D.W.; Buller, P.L.

    1985-01-08

    A carrier mounted seismic vibrator system that is primarily adapted for generation of high force, high frequency seismic energy into an earth medium. The apparatus includes first and second vibrators as supported by first and second lift systems disposed in tandem juxtaposition generally centrally in said vehicle, and the lift systems are designed to maintain equal hold-down force on the vibrator coupling baseplates without exceeding the weight of the carrier vehicle. The juxtaposed vibrators are then energized in synchronized relationship to propagate increased amounts of higher frequency seismic energy into an earth medium.

  10. Evaluation of shallow sediment methane cycling in a pockmark field on the Chatham Rise, New Zealand

    NASA Astrophysics Data System (ADS)

    Coffin, R. B.; Rose, P. S.; Klaucke, I.; Bialas, J.; Pecher, I. A.; Gorman, A. R.

    2014-12-01

    Seismic studies have identified an extensive field (>20,000 km2) of seafloor depressions, or pockmarks, on the southwestern flank of the Chatham Rise, New Zealand. It has been suggested that these pockmarks result from gas hydrate dissociation linked to sea-level changes during glacial-interglacial cycles. Gas hydrates are predominately composed of methane (CH4), a potent greenhouse gas. Surface sediment cores (~ 8 m) were collected from the pockmark field on the Chatham Rise during a research cruise in February 2013 to evaluate the association of the features with CH4 releases. A suite of geochemical parameters are interpreted to determine the methane contribution to solid phase sediment and pore water. The upward flux of CH4 in sediments is often quantified using pore water sulfate (SO42-) profiles, assuming steady-state consumption of SO42- and CH4 by anaerobic oxidation of methane (AOM): CH4 + SO42- → HCO3- + HS- + H2O. This reaction is one of the primary controls on CH4 distributions in sediments. This work will present pore water SO42-, sulfide (HS-) and chloride (Cl-) depth profiles in sediment collected from the pockmark field. Theoretical SO42- distributions in the absence of AOM are compared to observed SO42- profiles as a preliminary assessment of the influence of CH4 on sediment geochemistry in and around the seafloor depressions. In addition isotopically-light CH4 is incorporated into sediment carbon pools via AOM and subsequent CO2 fixation. Stable carbon isotope distributions in the organic and inorganic carbon pools are presented to determine the influence of CH4 in sediments in the vicinty of the pockmarks. Collectively, the geochemical data are used to assess the role of gas hydate dissociation in pockmark formation on the Chatham Rise. Despite sesimic data interpretation in this region there is no modern day contribution of CH4 to shallow sediment carbon cycling and data are presented to assess paleogeochemical methane cycling.

  11. Ni cycling in mangrove sediments from New Caledonia

    NASA Astrophysics Data System (ADS)

    Noel, V. S.; Morin, G.; Juillot, F.; Marchand, C.; Brest, J.; Bargar, J.; Munoz, M.; Ardo, S.; Brown, G. E.

    2014-12-01

    In New Caledonia, mangroves receive large inputs of lateritic materials eroded from massive ultramafic deposits enriched in Fe, Ni, Mn, Cr, and Co. Because of the major physicochemical gradients, especially redox gradients, that characterize these ecosystems, mineralogical transformations may influence the crystal-chemistry and bioavailability of Ni and its mobility towards a lagoon of over 20,000 km2. Bulk and spatially resolved chemical analyses by SEM-EDXS were coupled with Ni K-edge X-ray absorption fine structure (XAFS) spectroscopy analysis to characterize the vertical and lateral changes in Ni speciation across the intertidal zone of a mangrove forest in the Vavouto Bay (New Caledonia) where Ni concentrations range from 1000 to 5300 mg•kg-1. XAFS results indicate that phyllosilicates and goethite inherited from the eroded lateritic materials are the dominant Ni-bearing phases in the surface horizons of the mangrove sediments. They are fully preserved at depth in the dry and oxic salt flat area, located on the inland side of the coast. In contrast, beneath the vegetated Rhizophoras and Avicennias stands Ni-bearing goethites rapidly diminish with increasing depth in the anoxic horizons of the sediments, and pyrite and organic complexes become the dominant Ni-containing species. Moreover, Ni incorporation in pyrite is more developed in the sediments beneath the intermediate Avicennia stand than beneath the Rhizophora stand that is closest to the shore. Such lateral changes in Ni speciation may be related to reoxidation of Ni-bearing pyrites in the Rhizophora stand, which is subject to periodic alternation of reducing and oxidizing events due to tidal fluctuations. These major changes in Ni speciation could significantly influence Ni mobility across the interidal zone. Indeed, as estimated with respect to Ti concentration, which is taken as a geochemical invariant, Ni is found to be immobile in the salt flat, to accumulate beneath the Avicennia stand, and to

  12. Constraining magnesium cycling in marine sediments using magnesium isotopes

    NASA Astrophysics Data System (ADS)

    Higgins, J. A.; Schrag, D. P.

    2010-09-01

    Magnesium concentrations in deep-sea sediment pore-fluids typically decrease down core due to net precipitation of dolomite or clay minerals in the sediments or underlying crust. To better characterize and differentiate these processes, we have measured magnesium isotopes in pore-fluids and sediment samples from Ocean Drilling Program sites (1082, 1086, 1012, 984, 1219, and 925) that span a range of oceanographic settings. At all sites, magnesium concentrations decrease with depth. At sites where diagenetic reactions are dominated by the respiration of organic carbon, pore-fluid δ 26Mg values increase with depth by as much as 2‰. Because carbonates preferentially incorporate 24Mg (low δ 26Mg), the increase in pore-fluid δ 26Mg values at these sites is consistent with the removal of magnesium in Mg-carbonate (dolomite). In contrast, at sites where the respiration of organic carbon is not important and/or weatherable minerals are abundant, pore-fluid δ 26Mg values decrease with depth by up to 2‰. The decline in pore-fluid δ 26Mg at these sites is consistent with a magnesium sink that is isotopically enriched relative to the pore-fluid. The identity of this enriched magnesium sink is likely clay minerals. Using a simple 1D diffusion-advection-reaction model of pore-fluid magnesium, we estimate rates of net magnesium uptake/removal and associated net magnesium isotope fractionation factors for sources and sinks at all sites. Independent estimates of magnesium isotope fractionation during dolomite precipitation from measured δ 26Mg values of dolomite samples from sites 1082 and 1012 are very similar to modeled net fractionation factors at these sites, suggesting that local exchange of magnesium between sediment and pore-fluid at these sites can be neglected. Our results indicate that the magnesium incorporated in dolomite is 2.0-2.7‰ depleted in δ 26Mg relative to the precipitating fluid. Assuming local exchange of magnesium is minor at the rest of the

  13. Terrestrial sedimentation and the carbon cycle: coupling weathering and erosion to carbon burial

    USGS Publications Warehouse

    Stallard, R.F.

    1998-01-01

    This paper examines the linkages between the carbon cycle and sedimentary processes on land. Available data suggest that sedimentation on land can bury vast quantities of organic carbon, roughly 1015 g C yr-1. To evaluate the relative roles of various classes of processes in the burial of carbon on land, terrestrial sedimentation was modeled as a series of 864 scenarios. Each scenario represents a unique choice of intensities for seven classes of processes and two different global wetland distributions. Comparison was made with presumed preagricultural conditions. The classes of processes were divided into two major component parts: clastic sedimentation of soil-derived carbon and organic sedimentation of autochthonous carbon. For clastic sedimentation, masses of sediment were considered for burial as reservoir sediment, lake sediment, and combined colluvium, alluvium, and aeolian deposits. When the ensemble of models is examined, the human-induced burial of 0.6-1.5.1015 g yr-1 of carbon on land is entirely plausible. This sink reaches its maximum strength between 30 ?? and 50??N. Paddy lands stand out as a type of land use that warrants future study, but the many faces of rice agriculture limit generalization. In an extreme scenario, paddy lands alone could be made to bury about 1.1015 g C yr-1. Arguing that terrestrial sedimentation processes could be much of the sink for the so called 'missing carbon' is reasonable. Such a hypothesis, however, requires major redesign of how the carbon cycle is modeled. Unlike ecosystem processes that are amenable to satellite monitoring and parallel modeling, many aspects of terrestrial sedimentation are hidden from space.

  14. H2 cycling and microbial bioenergetics in anoxic sediments

    NASA Technical Reports Server (NTRS)

    Hoehler, Tori M.; DeVincenzi, Donald (Technical Monitor)

    2001-01-01

    The simple biochemistry of H2 is central to a large number of microbial processes, affecting the interaction of organisms with each other and with the environment. In anoxic sediments, the great majority of microbial redox processes involve H2 as a reactant, product, or potential by-product, and the thermodynamics of these processes are thus highly sensitive to fluctuations in environmental H2 concentrations. In turn, H2 concentrations are controlled by the activity of H2-consuming microorganisms, which efficiently utilize this substrate down to levels which correspond to their bioenergetic limitations. Consequently, any environmental change which impacts the thermodynamics of H2-consuming organisms is mirrored by a corresponding change in H2 concentrations. This phenomenon is illustrated in anoxic sediments from Cape Lookout Bight, NC, USA: H2 concentrations are controlled by a suite of environmental parameters (e.g., temperature, sulfate concentrations) in a fashion which can be quantitatively described by a simple thermodynamic model. These findings allow us to calculate the apparent minimum quantity of biologically useful energy in situ. We find that sulfate reducing bacteria are not active at energy yields below -18 kJ per mole sulfate, while methanogenic archaea exhibit a minimum close to -10 kJ per mole methane.

  15. CYCLING OF XENOBIOTICS THROUGH MARINE AND ESTUARINE SEDIMENTS

    EPA Science Inventory

    The results of five broadly defined projects are reported. Cycling of xenobiotics was studied with a photo-bioassay system that used time-lapse photography to evaluate effects of Kepone and sodium pentachlorophenate on feeding activity of the lugworm, Arenicola cristata. Radio-la...

  16. Sea-level responses to sediment transport over the last ice age cycle

    NASA Astrophysics Data System (ADS)

    Ferrier, K.; Mitrovica, J. X.

    2013-12-01

    Sea-level changes over the last ice age cycle were instrumental in steering Earth's topographic evolution. These sea-level variations were driven by changes in surface mass loads, including not only ice and ocean mass variations but also the transfer of rock from eroding mountains to sedimentary deposits. Here we use an extended numerical model of ice age sea level (Dalca et al., 2013) to explore how sediment erosion and deposition affected global sea-level variations over the last ice age cycle. The model takes histories of ice and sediment loads as inputs, and it computes gravitationally self-consistent sea level responses by accounting for the deformational, gravitational, and rotational perturbations in the Earth's viscoelastic form. In these model simulations, we use published estimates of erosion rates, sedimentation rates, and ice sheet variations to constrain sediment and ice loading since the Last Interglacial. We explore sea-level responses to several erosional and depositional scenarios, and in each we quantify the relative contributions of crustal deformation and gravitational perturbation to the computed sea-level change. We also present a case study to illustrate the effects that sediment transfer can have on sea level at the regional scale. In particular, we focus on the region surrounding the Indus River, where fluvial sediment fluxes are among the highest on Earth. Preliminary model results suggest that sediment fluxes from Asia to the ocean are large enough to produce a significant response in sea level along the northeastern coast of the Arabian Sea. Moreover, they suggest that modeled sea-level histories are sensitive to the timing and spatial distribution of sediment erosion and deposition. For instance, sediment deposition along the continental shelf - which may have been the primary site of Indus River sediment deposition during the Holocene - produces a different sea-level response than sediment deposition on the deep-sea Indus Fan, where

  17. High frequency power distribution system

    NASA Technical Reports Server (NTRS)

    Patel, Mikund R.

    1986-01-01

    The objective of this project was to provide the technology of high frequency, high power transmission lines to the 100 kW power range at 20 kHz frequency. In addition to the necessary design studies, a 150 m long, 600 V, 60 A transmission line was built, tested and delivered for full vacuum tests. The configuration analysis on five alternative configurations resulted in the final selection of the three parallel Litz straps configuration, which gave a virtually concentric design in the electromagnetic sense. Low inductance, low EMI and flexibility in handling are the key features of this configuration. The final design was made after a parametric study to minimize the losses, weight and inductance. The construction of the cable was completed with no major difficulties. The R,L,C parameters measured on the cable agreed well with the calculated values. The corona tests on insulation samples showed a safety factor of 3.

  18. New chronology for the southern Kalahari Group sediments with implications for sediment-cycle dynamics and early hominin occupation

    NASA Astrophysics Data System (ADS)

    Matmon, Ari; Hidy, Alan J.; Vainer, Shlomy; Crouvi, Onn; Fink, David; Erel, Yigal; Arnold, M.; Aumaître, G.; Bourlès, D.; Keddadouche, K.; Horwitz, Liora K.; Chazan, Michael

    2015-07-01

    Kalahari Group sediments accumulated in the Kalahari basin, which started forming during the breakup of Gondwana in the early Cretaceous. These sediments cover an extensive part of southern Africa and form a low-relief landscape. Current models assume that the Kalahari Group accumulated throughout the entire Cenozoic. However, chronology has been restricted to early-middle Cenozoic biostratigraphic correlations and to OSL dating of only the past ~ 300 ka. We present a new chronological framework that reveals a dynamic nature of sedimentation in the southern Kalahari. Cosmogenic burial ages obtained from a 55 m section of Kalahari Group sediments from the Mamatwan Mine, southern Kalahari, indicate that the majority of deposition at this location occurred rapidly at 1-1.2 Ma. This Pleistocene sequence overlies the Archaean basement, forming a significant hiatus that permits the possibility of many Phanerozoic cycles of deposition and erosion no longer preserved in the sedimentary record. Our data also establish the existence of a shallow early-middle Pleistocene water body that persisted for > 450 ka prior to this rapid period of deposition. Evidence from neighboring archeological excavations in southern Africa suggests an association of high-density hominin occupation with this water body.

  19. New chronology for the southern Kalahari Group sediments - implications for sediment-cycle dynamics and basin development

    NASA Astrophysics Data System (ADS)

    Matmon, Ari; Hidy, Alan; Vainer, Shlomy; Crouvi, Onn; Fink, David; Erel, Yigal; Aster Team; Horwitz, Liora; Chazan, Michael

    2016-04-01

    Kalahari Group sediments accumulated in the Kalahari basin, which started forming during the breakup of Gondwana in the early Cretaceous. These sediments cover an extensive part of southern Africa and form a low-relief landscape. Current models assume that the Kalahari Group accumulated throughout the entire Cenozoic. However, chronology has been restricted to early-middle Cenozoic biostratigraphic correlations and to OSL dating of only the past ~300 ka. We present a new chronological framework that reveals a dynamic nature of sedimentation in the southern Kalahari. Cosmogenic burial ages obtained from a 55 m section of Kalahari Group sediments from the Mamatwan Mine, southern Kalahari, indicate that the majority of deposition at this location occurred rapidly at 1-1.2 Ma. This Pleistocene sequence overlies the Archaean basement, forming a significant hiatus that permits the possibility of many Phanerozoic cycles of deposition and erosion no longer preserved in the sedimentary record. Our data also establish the existence of a shallow early-middle Pleistocene water body that persisted for >450 ka prior to this rapid period of deposition and suggesting an Okavango-like environment. Evidence from neighboring archaeological excavations in southern Africa suggests an association of high-density hominin occupation with this water body.

  20. Temperature and Cyanobacterial Bloom Biomass Influence Phosphorous Cycling in Eutrophic Lake Sediments

    PubMed Central

    Chen, Mo; Ye, Tian-Ran; Krumholz, Lee R.; Jiang, He-Long

    2014-01-01

    Cyanobacterial blooms frequently occur in freshwater lakes, subsequently, substantial amounts of decaying cyanobacterial bloom biomass (CBB) settles onto the lake sediments where anaerobic mineralization reactions prevail. Coupled Fe/S cycling processes can influence the mobilization of phosphorus (P) in sediments, with high releases often resulting in eutrophication. To better understand eutrophication in Lake Taihu (PRC), we investigated the effects of CBB and temperature on phosphorus cycling in lake sediments. Results indicated that added CBB not only enhanced sedimentary iron reduction, but also resulted in a change from net sulfur oxidation to sulfate reduction, which jointly resulted in a spike of soluble Fe(II) and the formation of FeS/FeS2. Phosphate release was also enhanced with CBB amendment along with increases in reduced sulfur. Further release of phosphate was associated with increases in incubation temperature. In addition, CBB amendment resulted in a shift in P from the Fe-adsorbed P and the relatively unreactive Residual-P pools to the more reactive Al-adsorbed P, Ca-bound P and organic-P pools. Phosphorus cycling rates increased on addition of CBB and were higher at elevated temperatures, resulting in increased phosphorus release from sediments. These findings suggest that settling of CBB into sediments will likely increase the extent of eutrophication in aquatic environments and these processes will be magnified at higher temperatures. PMID:24682039

  1. Pyrosequencing evidence for iron-cycling microbial communities in sediments of the Skagerrak and Bothnian Bay

    NASA Astrophysics Data System (ADS)

    Reyes, Carolina; Dellwig, Olaf; Noriega-Ortega, Beatriz; Dähnke, Kirstin; Gehre, Matthias; Böttcher, Michael E.; Friedrich, Michael W.

    2015-04-01

    The diversity and metabolic pathways of microorganisms linked to Fe cycling in marine sediments are still poorly understood. Marine microorganisms in general are difficult to isolate and those that have been successfully isolated may not represent the main endogenous population. Various culture-independent techniques have been applied to characterize marine microbial communities, but only recently, has high throughput pyrosequencing been applied in marine sediment studies. Initial results are promising in capturing the full complexity of microbial communities in sediments. We performed a pyrosequencing-based study in marine and brackish sediments of the Baltic Sea; to our knowledge this is the first pyrosequencing study focused on the zone of Fe cycling. The goal of this study was to determine the bacterial and archaeal community composition near the sediment surface showing ongoing Fe cycling as a first step in characterizing the microorganisms potentially involved in Fe cycling. Two 35-cm-cores were sampled from ferruginous sediments in the Skagerrak, SK, North-Baltic Sea and the Bothnian Bay, BB, Northern Baltic Sea. Porewater (Fe2+, Mn2+, SO42-) and solid phase (Fe, Mn, total S) concentrations were measured and 16S rRNA genes were analysed using 454-pyrosequencing. Additionally, stable S and O isotope signatures of dissolved sulfate were measured at SK site. Sediment biogeochemistry indicated an intense suboxic zone with accumulation of dissolved Fe in the top 30 cm but only minor net sulfate (SO42-) reduction at both sites. Pore water profiles showed Fe2+ and Mn2+ levels of ~140-150 µM throughout the core below a 6 cm thick oxidized surface layer in SK sediments and ~300 µM below a 2 cm thick surface layer in BB sediments. Dissolved sulfide levels were below the detection limit in both sediments. Stable S and O isotope signatures suggest only minor net sulfate reduction. Fe reduction in the studied sediments is dominated by microbial dissimilatory Fe

  2. MODELING NITROGEN-CARBON CYCLING AND OXYGEN CONSUMPTION IN BOTTOM SEDIMENTS

    EPA Science Inventory

    A model framework is presented for simulating nitrogen and carbon cycling at the sediment–water interface, and predicting oxygen consumption by oxidation reactions inside the sediments. Based on conservation of mass and invoking simplifying assumptions, a coupled system of diffus...

  3. High frequency dynamic nuclear polarization.

    PubMed

    Ni, Qing Zhe; Daviso, Eugenio; Can, Thach V; Markhasin, Evgeny; Jawla, Sudheer K; Swager, Timothy M; Temkin, Richard J; Herzfeld, Judith; Griffin, Robert G

    2013-09-17

    During the three decades 1980-2010, magic angle spinning (MAS) NMR developed into the method of choice to examine many chemical, physical, and biological problems. In particular, a variety of dipolar recoupling methods to measure distances and torsion angles can now constrain molecular structures to high resolution. However, applications are often limited by the low sensitivity of the experiments, due in large part to the necessity of observing spectra of low-γ nuclei such as the I = 1/2 species (13)C or (15)N. The difficulty is still greater when quadrupolar nuclei, such as (17)O or (27)Al, are involved. This problem has stimulated efforts to increase the sensitivity of MAS experiments. A particularly powerful approach is dynamic nuclear polarization (DNP) which takes advantage of the higher equilibrium polarization of electrons (which conventionally manifests in the great sensitivity advantage of EPR over NMR). In DNP, the sample is doped with a stable paramagnetic polarizing agent and irradiated with microwaves to transfer the high polarization in the electron spin reservoir to the nuclei of interest. The idea was first explored by Overhauser and Slichter in 1953. However, these experiments were carried out on static samples, at magnetic fields that are low by current standards. To be implemented in contemporary MAS NMR experiments, DNP requires microwave sources operating in the subterahertz regime, roughly 150-660 GHz, and cryogenic MAS probes. In addition, improvements were required in the polarizing agents, because the high concentrations of conventional radicals that are required to produce significant enhancements compromise spectral resolution. In the last two decades, scientific and technical advances have addressed these problems and brought DNP to the point where it is achieving wide applicability. These advances include the development of high frequency gyrotron microwave sources operating in the subterahertz frequency range. In addition, low

  4. High Frequency Dynamic Nuclear Polarization

    PubMed Central

    Ni, Qing Zhe; Daviso, Eugenio; Can, Thach V.; Markhasin, Evgeny; Jawla, Sudheer K.; Swager, Timothy M.; Temkin, Richard J.; Herzfeld, Judith; Griffin, Robert G.

    2013-01-01

    Conspectus During the three decades 1980–2010, magic angle spinning (MAS) NMR developed into the method of choice to examine many chemical, physical and biological problems. In particular, a variety of dipolar recoupling methods to measure distances and torsion angles can now constrain molecular structures to high resolution. However, applications are often limited by the low sensitivity of the experiments, due in large part to the necessity of observing spectra of low-γ nuclei such as the I = ½ species 13C or 15N. The difficulty is still greater when quadrupolar nuclei, like 17O or 27Al, are involved. This problem has stimulated efforts to increase the sensitivity of MAS experiments. A particularly powerful approach is dynamic nuclear polarization (DNP) which takes advantage of the higher equilibrium polarization of electrons (which conventionally manifests in the great sensitivity advantage of EPR over NMR). In DNP, the sample is doped with a stable paramagnetic polarizing agent and irradiated with microwaves to transfer the high polarization in the electron spin reservoir to the nuclei of interest. The idea was first explored by Overhauser and Slichter in 1953. However, these experiments were carried out on static samples, at magnetic fields that are low by current standards. To be implemented in contemporary MAS NMR experiments, DNP requires microwave sources operating in the subterahertz regime — roughly 150–660 GHz — and cryogenic MAS probes. In addition, improvements were required in the polarizing agents, because the high concentrations of conventional radicals that are required to produce significant enhancements compromise spectral resolution. In the last two decades scientific and technical advances have addressed these problems and brought DNP to the point where it is achieving wide applicability. These advances include the development of high frequency gyrotron microwave sources operating in the subterahertz frequency range. In addition, low

  5. Special Aspects in Designing High - Frequency Betatron

    NASA Astrophysics Data System (ADS)

    Filimonov, A. A.; Kasyanov, S. V.; Kasyanov, V. A.

    2016-01-01

    The article is devoted to designing the high - frequency betatron. In high - frequency betatron most important problem is overheating of the elements of the body radiator unit. In an article some directions of solving this problem are shown.

  6. Hydraulic and sediment transport properties of autogenic avulsion cycles on submarine fans with supercritical distributaries

    NASA Astrophysics Data System (ADS)

    Hamilton, Paul B.; Strom, Kyle B.; Hoyal, David C. J. D.

    2015-07-01

    Submarine fans, like other distributive systems, are built by repeated avulsion cycles. However, relative to deltas and alluvial fans, much less is known about avulsions in subaqueous settings. In this study, we ran a set of subaqueous fan experiments to investigate the mechanics associated with autogenic avulsion cycles of self-formed channels and lobe deposits on steep slopes. The experiments used saline density currents with crushed plastic to emulate sustained turbidity currents and bed load transport. We collected detailed hydraulic and bathymetric measurements and made use of a 1-D laterally expanding density current model to better understand different aspects of the avulsion cycle. Our results reveal three major components of the avulsion cycles: (1) distributary channel incision, extension, and stagnation; (2) mouth bar aggradation and hydraulic jump initiation; and (3) hydraulic jump sedimentation and upstream retreat. Interestingly, in all but one experiment, the avulsion cycles led to fans that remained perched above the basin slope break. Experimental data and hydraulic theory were used to unravel actual mechanics associated with cycles. We found that channels stopped extending into the basin due to a decay in sediment transport capacity relative to sediment supply and that the reduction in capacity was primarily an outcome of expansion-driven velocity reduction; dilution played a secondary role. Once channel extension ceased, mouth bar deposits aggraded to a thickness approximately equal to the critical step height needed to create a choked flow condition. The choke then initiated a hydraulic jump on the upstream side of the bar. Once formed, the jump detained a majority of the incoming sediment and forced the channel-to-lobe transition upstream, filling the channel with steep backset bedding and capping the entire channel with a mounded lobate deposit. These intrinsic processes repeated through multiple avulsion cycles to build the fan.

  7. Short-chain alkane cycling in deep Gulf of Mexico cold-seep sediments

    NASA Astrophysics Data System (ADS)

    Sibert, R.; Joye, S. B.; Hunter, K.

    2015-12-01

    Mixtures of light hydrocarbon gases are common in deep Gulf of Mexico cold-seep sediments, and are typically dissolved in pore fluids, adsorbed to sediment particles, trapped in methane ice, or as free gas. The dominant component in these natural gas mixtures is usually methane (>80% C1), but ethane (C2) and propane (C3) are nearly always present in trace amounts (<1% total). The processes that control the concentration and isotopic signature of these gases in sediments are well explained for methane, but the controls for C2/C3 cycling are still a relative mystery. Methane production proceeds in deep anoxic sediments by either 1) thermocatalytic cracking of fossil organic matter, or 2) as a direct product of microbial metabolism, i.e. methanogenesis. In surface sediments, it appears that both microbial consumption and chemical deposition of methane (i.e. as methane clathrate) ensures that >95% of the methane produced at depth never reaches the water column. Production of C1 and C2 in deep-sea sediments has been historically attributed only to thermocatalytic processes, though limited data suggests production of C2/C3 compounds through the activity of archaea at depth. Furthermore, carbon isotopic data on ethane and propane from deep cores of Gulf of Mexico sediments suggest alkanogenesis at >3 m depth in the sediment column and alkane oxidation in uppermost oxidant-rich sediments. Additional studies have also isolated microorganisms capable of oxidizing ethane and propane in the laboratory, but field studies of microbial-driven dynamics of C2/C3 gases in cold-seep sediments are rare. Here, we present the results of a series of incubation experiments using sediment slurries culled from surface sediments from one of the most prolific natural oil and gas seeps in the Gulf of Mexico. Rates of alkane oxidation were measured under a variety of conditions to assess the surface-driven microbial controls on C2/C3 cycling in cold-seep environments. Such microbial processes

  8. Organic matter remineralization predominates phosphorus cycling in the mid-Bay sediments in the Chesapeake Bay.

    PubMed

    Joshi, Sunendra R; Kukkadapu, Ravi K; Burdige, David J; Bowden, Mark E; Sparks, Donald L; Jaisi, Deb P

    2015-05-19

    Chesapeake Bay, the largest and most productive estuary in the U.S., suffers from varying degrees of water quality issues fueled by both point and nonpoint nutrient sources. Restoration of the Bay is complicated by the multitude of nutrient sources, their variable inputs, and complex interaction between imported and regenerated nutrients. These complexities not only restrict formulation of effective restoration plans but also open up debates on accountability issues with nutrient loading. A detailed understanding of sediment phosphorus (P) dynamics provides information useful in identifying the exchange of dissolved constituents across the sediment-water interface as well as helps to better constrain the mechanisms and processes controlling the coupling between sediments and the overlying waters. Here we used phosphate oxygen isotope ratios (δ(18)O(P)) in concert with sediment chemistry, X-ray diffraction, and Mössbauer spectroscopy on sediments retrieved from an organic rich, sulfidic site in the mesohaline portion of the mid-Bay to identify sources and pathway of sedimentary P cycling and to infer potential feedbacks on bottom water hypoxia and surface water eutrophication. Authigenic phosphate isotope data suggest that the regeneration of inorganic P from organic matter degradation (remineralization) is the predominant, if not sole, pathway for authigenic P precipitation in the mid-Bay sediments. This indicates that the excess inorganic P generated by remineralization should have overwhelmed any pore water and/or bottom water because only a fraction of this precipitates as authigenic P. This is the first research that identifies the predominance of remineralization pathway and recycling of P within the Chesapeake Bay. Therefore, these results have significant implications on the current understanding of sediment P cycling and P exchange across the sediment-water interface in the Bay, particularly in terms of the sources and pathways of P that sustain hypoxia

  9. High-Frequency Inductor Materials

    NASA Astrophysics Data System (ADS)

    Varga, L. K.

    2014-01-01

    The Finemet-type nanocrystalline alloy represents an advanced soft-magnetic metal-metal-type nanocomposite with an eddy-current-determined high- frequency limit. A survey of different heat treatments under tensile stress is presented to tailor the hysteresis loop by induced transversal anisotropy. The flattened loop having reduced effective permeability enhances the eddy- current limit in the MHz region; For example, continuous stress annealing in a tubular furnace of 1 m length at 650°C, pulling the ribbon with a velocity of 4 m/min under a tensile stress of 200 MPa, results in a wound core having a permeability of 120 and a frequency limit of 10 MHz. Careful annealing preserves the static coercivity below 10 A/m. The power loss at 0.1 T and 100 kHz is only 82 mW/cm3, which is an order of magnitude lower then the values obtained for Sendust™ cores in similar conditions.

  10. High frequency-heated air turbojet

    NASA Technical Reports Server (NTRS)

    Miron, J. H. D.

    1986-01-01

    A description is given of a method to heat air coming from a turbojet compressor to a temperature necessary to produce required expansion without requiring fuel. This is done by high frequency heating, which heats the walls corresponding to the combustion chamber in existing jets, by mounting high frequency coils in them. The current transformer and high frequency generator to be used are discussed.

  11. Organic Matter Remineralization Predominates Phosphorus Cycling in the Mid-Bay Sediments in the Chesapeake Bay

    SciTech Connect

    Sunendra, Joshi R.; Kukkadapu, Ravi K.; Burdige, David J.; Bowden, Mark E.; Sparks, Donald L.; Jaisi, Deb P.

    2015-05-19

    The Chesapeake Bay, the largest and most productive estuary in the US, suffers from varying degrees of water quality issues fueled by both point and non–point source nutrient sources. Restoration of the bay is complicated by the multitude of nutrient sources, their variable inputs and hydrological conditions, and complex interacting factors including climate forcing. These complexities not only restrict formulation of effective restoration plans but also open up debates on accountability issues with nutrient loading. A detailed understanding of sediment phosphorus (P) dynamics enables one to identify the exchange of dissolved constituents across the sediment- water interface and aid to better constrain mechanisms and processes controlling the coupling between the sediments and the overlying waters. Here we used phosphate oxygen isotope ratios (δ18Op) in concert with sediment chemistry, XRD, and Mössbauer spectroscopy on the sediment retrieved from an organic rich, sulfidic site in the meso-haline portion of the mid-bay to identify sources and pathway of sedimentary P cycling and to infer potential feedback effect on bottom water hypoxia and surface water eutrophication. Isotope data indicate that the regeneration of inorganic P from organic matter degradation (remineralization) is the predominant, if not sole, pathway for authigenic P precipitation in the mid-bay sediments. We interpret that the excess inorganic P generated by remineralization should have overwhelmed any bottom-water and/or pore-water P derived from other sources or biogeochemical processes and exceeded saturation with respect to authigenic P precipitation. It is the first research that identifies the predominance of remineralization pathway against remobilization (coupled Fe-P cycling) pathway in the Chesapeake Bay. Therefore, these results are expected to have significant implications for the current understanding of P cycling and benthic-pelagic coupling in the bay, particularly on the

  12. Pressurized high frequency thermoacoustic engines

    NASA Astrophysics Data System (ADS)

    Webb, Nicholas D.

    Acoustic heat engines show much promise for converting waste heat to electricity. Since most applications require high power levels, high frequency thermoacoustic engines can reach such performance by operating with a pressurized working gas. Results on a 3 kHz prime mover, consisting of a quarter-wave resonator and a random stack material between two heat exchangers, show that the acoustic power from such a device is raised substantially as the working gas is pressurized. At pressures up to approximately 10 bar, the increase in acoustic power is approximately linear to the increase in pressure, and thus is an effective way to increase the power output of thermoacoustic engines. Since the heat input was not changed during the experiments, the increases in acoustic power translate directly to increases in engine efficiency which is calculated as the output acoustic power divided by the input heat power. In most experiments run in this study, the engine efficiency increased by a factor of at least 4 as the pressure was increased from 2 bar up to about 10 bar. Further increases in pressure lead to acoustic power saturation and eventual attenuation. This is most likely due to a combination of several factors including the shrinking thermal penetration depth, and the fact that the losses increase faster with pressure in a random stack material than in traditional parallel plates. Pressurization also leads to a lower DeltaT for onset of oscillations in the range of 10 bar of mean pressure, potentially opening up even more heat sources that can power a thermoacoustic engine. Results from another 3 kHz engine, one that was pressurized itself as opposed to being placed in a pressurized chamber, are also presented. The configuration of this engine solves the problem of how to simultaneously pressurize the engine and inject heat into the hot heat exchanger. It was also noted that the geometry of the resonator cavity in the quarter wavelength pressurized engine plays an

  13. LOSCAR: Long-term Ocean-atmosphere-Sediment CArbon cycle Reservoir Model

    NASA Astrophysics Data System (ADS)

    Zeebe, R. E.

    2011-06-01

    The LOSCAR model is designed to efficiently compute the partitioning of carbon between ocean, atmosphere, and sediments on time scales ranging from centuries to millions of years. While a variety of computationally inexpensive carbon cycle models are already available, many are missing a critical sediment component, which is indispensable for long-term integrations. One of LOSCAR's strengths is the coupling of ocean-atmosphere routines to a computationally efficient sediment module. This allows, for instance, adequate computation of CaCO3 dissolution, calcite compensation, and long-term carbon cycle fluxes, including weathering of carbonate and silicate rocks. The ocean component includes various biogeochemical tracers such as total carbon, alkalinity, phosphate, oxygen, and stable carbon isotopes. We have previously published applications of the model tackling future projections of ocean chemistry and weathering, pCO2 sensitivity to carbon cycle perturbations throughout the Cenozoic, and carbon/calcium cycling during the Paleocene-Eocene Thermal Maximum. The focus of the present contribution is the detailed description of the model including numerical architecture, processes and parameterizations, tuning, and examples of input and output. Typical CPU integration times of LOSCAR are of order seconds for several thousand model years on current standard desktop machines. The LOSCAR source code in C can be obtained from the author by sending a request to loscar.model@gmail.com.

  14. Use of life cycle assessments to evaluate the environmental footprint of contaminated sediment remediation.

    PubMed

    Sparrevik, Magnus; Saloranta, Tuomo; Cornelissen, Gerard; Eek, Espen; Fet, Annik Magerholm; Breedveld, Gijs D; Linkov, Igor

    2011-05-15

    Ecological and human risks often drive the selection of remedial alternatives for contaminated sediments. Traditional human and ecological risk assessment (HERA) includes assessing risk for benthic organisms and aquatic fauna associated with exposure to contaminated sediments before and after remediation as well as risk for human exposure but does not consider the environmental footprint associated with implementing remedial alternatives. Assessment of environmental effects over the whole life cycle (i.e., Life Cycle Assessment, LCA) could complement HERA and help in selecting the most appropriate sediment management alternative. Even though LCA has been developed and applied in multiple environmental management cases, applications to contaminated sediments and marine ecosystems are in general less frequent. This paper implements LCA methodology for the case of the polychlorinated dibenzo-p-dioxins and -furans (PCDD/F)-contaminated Grenland fjord in Norway. LCA was applied to investigate the environmental footprint of different active and passive thin-layer capping alternatives as compared to natural recovery. The results showed that capping was preferable to natural recovery when analysis is limited to effects related to the site contamination. Incorporation of impacts related to the use of resources and energy during the implementation of a thin layer cap increase the environmental footprint by over 1 order of magnitude, making capping inferior to the natural recovery alternative. Use of biomass-derived activated carbon, where carbon dioxide is sequestered during the production process, reduces the overall environmental impact to that of natural recovery. The results from this study show that LCA may be a valuable tool for assessing the environmental footprint of sediment remediation projects and for sustainable sediment management. PMID:21520943

  15. High frequency microbubble-switched oscillations modulated by microfluidic transistors

    NASA Astrophysics Data System (ADS)

    Yang, Fanghao; Dai, Xianming; Li, Chen

    2012-08-01

    Creating high frequency two-phase oscillations (HF-TPOs) remains an important goal in advancing microscale fluidic logic devices, micro-mixers, micro-actuators, and flow controls. However, thermally driven TPO frequency has been hindered by confinements of compressible vapor bubbles and low thermal diffusivity in microfluidic systems. In this study, a mechanism creating high frequency microbubbles growth/collapse cycle has been developed to achieve HF-TPOs. A "microfluidic transistor" was conceptualized and fabricated to passively sustain and modulate HF-TPOs. Three orders of magnitude higher TPO frequency has been achieved compared to TPOs reported in literatures under similar working conditions.

  16. Lightweight, high-frequency transformers

    NASA Technical Reports Server (NTRS)

    Schwarze, G. E.

    1983-01-01

    The 25-kVA space transformer was developed under contract by Thermal Technology Laboratory, Buffalo, N. Y. The NASA Lewis transformer technology program attempted to develop the baseline technology. For the 25-kVA transformer the input voltage was chosen as 200 V, the output voltage as 1500 V, the input voltage waveform as square wave, the duty cycle as continuous, the frequency range (within certain constraints) as 10 to 40 kHz, the operating temperatures as 85 deg. and 130 C, the baseplate temperature as 50 C, the equivalent leakage inductance as less than 10 micro-h, the operating environment as space, and the life expectancy as 10 years. Such a transformer can also be used for aircraft, ship and terrestrial applications.

  17. Effects of global climate change and organic pollution on nutrient cycling in marine sediments

    NASA Astrophysics Data System (ADS)

    Sanz-Lázaro, C.; Valdemarsen, T.; Holmer, M.

    2015-01-01

    Increasing ocean temperature due to climate change is an important anthropogenic driver of ecological change in coastal systems, where sediments play a major role in nutrient cycling. Our ability to predict ecological consequences of climate change is enhanced by simulating real scenarios especially when the interactions among drivers may not be just additive. Based on predicted climate change scenarios, we tested the effect of temperature and organic pollution on nutrient release from coastal sediments to the water column in a mesocosm experiment. PO43- release rates from sediments followed the same trends as organic matter mineralization rates, and increased linearly with temperature and were significantly higher under organic pollution than under non-polluted conditions. NH4+ release only increased significantly when the temperature rise was above 6 °C, and was significantly higher in organic polluted compared to non-polluted sediments. Nutrient release to the water column was only a fraction from the mineralized organic matter, suggesting PO43- retention and NH4+ oxidation in the sediment. Bioturbation and bioirrigation appeared to be key processes responsible of this behaviour. Considering that the primary production of most marine basins is N-limited, the excess release of NH4+ at temperature rise >6 ° could enhance water column primary productivity, which may lead to the deterioration of the environmental quality. Climate change effects are expected to be accelerated in areas affected by organic pollution.

  18. Effects of temperature and organic pollution on nutrient cycling in marine sediments

    NASA Astrophysics Data System (ADS)

    Sanz-Lazaro, C.; Valdemarsen, T.; Holmer, M.

    2015-08-01

    Increasing ocean temperature due to climate change is an important anthropogenic driver of ecological change in coastal systems. In these systems sediments play a major role in nutrient cycling. Our ability to predict ecological consequences of climate change is enhanced by simulating real scenarios. Based on predicted climate change scenarios, we tested the effect of temperature and organic pollution on nutrient release from coastal sediments to the water column in a mesocosm experiment. PO43- release rates from sediments followed the same trends as organic matter mineralization rates, increased linearly with temperature and were significantly higher under organic pollution than under nonpolluted conditions. NH4+ release only increased significantly when the temperature rise was above 6 °C, and it was significantly higher in organic polluted compared to nonpolluted sediments. Nutrient release to the water column was only a fraction from the mineralized organic matter, suggesting PO43- retention and NH4+ oxidation in the sediment. Bioturbation and bioirrigation appeared to be key processes responsible for this behavior. Considering that the primary production of most marine basins is N-limited, the excess release of NH4+ at a temperature rise > 6 °C could enhance water column primary productivity, which may lead to the deterioration of the environmental quality. Climate change effects are expected to be accelerated in areas affected by organic pollution.

  19. Downslope Eulerian mean flow associated with high-frequency current fluctuations observed on the outer continental shelf and upper slope along the northeastern United States continental margin: implications for sediment transport

    USGS Publications Warehouse

    Butman, B.

    1988-01-01

    Eulerian current measurements made 5-7 m above bottom at six stations along the United States east coast continental margin show a net downslope flow of 1-5 cm s-1. Although the scalar current speed decreases with water depth and toward the bottom, fluctuations in the cross-isobath flow were stronger and increasingly asymmetric near the bottom. Maximum downslope flow exceeded maximum upslope flow by a factor of two to three. The strength of the low-passed downslope flow was proportional to the upslope Reynolds flux of density as well as to the amplitude of the current fluctuations that have periods shorter than 30 h. These flow characteristics may be caused by differential vertical mixing in the bottom boundary layer where a stratified fluid flows upslope (unstable) and downslope (stable). The asymmetry in current strength clearly favors net downslope transport of sediments that move as bedload. ?? 1988.

  20. The Link between Microbial Diversity and Nitrogen Cycling in Marine Sediments Is Modulated by Macrofaunal Bioturbation

    PubMed Central

    Yazdani Foshtomi, Maryam; Braeckman, Ulrike; Derycke, Sofie; Sapp, Melanie; Van Gansbeke, Dirk; Sabbe, Koen; Willems, Anne; Vincx, Magda; Vanaverbeke, Jan

    2015-01-01

    Objectives The marine benthic nitrogen cycle is affected by both the presence and activity of macrofauna and the diversity of N-cycling microbes. However, integrated research simultaneously investigating macrofauna, microbes and N-cycling is lacking. We investigated spatio-temporal patterns in microbial community composition and diversity, macrofaunal abundance and their sediment reworking activity, and N-cycling in seven subtidal stations in the Southern North Sea. Spatio-Temporal Patterns of the Microbial Communities Our results indicated that bacteria (total and β-AOB) showed more spatio-temporal variation than archaea (total and AOA) as sedimentation of organic matter and the subsequent changes in the environment had a stronger impact on their community composition and diversity indices in our study area. However, spatio-temporal patterns of total bacterial and β-AOB communities were different and related to the availability of ammonium for the autotrophic β-AOB. Highest bacterial richness and diversity were observed in June at the timing of the phytoplankton bloom deposition, while richness of β-AOB as well as AOA peaked in September. Total archaeal community showed no temporal variation in diversity indices. Macrofauna, Microbes and the Benthic N-Cycle Distance based linear models revealed that, independent from the effect of grain size and the quality and quantity of sediment organic matter, nitrification and N-mineralization were affected by respectively the diversity of metabolically active β-AOB and AOA, and the total bacteria, near the sediment-water interface. Separate models demonstrated a significant and independent effect of macrofaunal activities on community composition and richness of total bacteria, and diversity indices of metabolically active AOA. Diversity of β-AOB was significantly affected by macrofaunal abundance. Our results support the link between microbial biodiversity and ecosystem functioning in marine sediments, and provided

  1. Plant Responses to High Frequency Electromagnetic Fields.

    PubMed

    Vian, Alain; Davies, Eric; Gendraud, Michel; Bonnet, Pierre

    2016-01-01

    High frequency nonionizing electromagnetic fields (HF-EMF) that are increasingly present in the environment constitute a genuine environmental stimulus able to evoke specific responses in plants that share many similarities with those observed after a stressful treatment. Plants constitute an outstanding model to study such interactions since their architecture (high surface area to volume ratio) optimizes their interaction with the environment. In the present review, after identifying the main exposure devices (transverse and gigahertz electromagnetic cells, wave guide, and mode stirred reverberating chamber) and general physics laws that govern EMF interactions with plants, we illustrate some of the observed responses after exposure to HF-EMF at the cellular, molecular, and whole plant scale. Indeed, numerous metabolic activities (reactive oxygen species metabolism, α- and β-amylase, Krebs cycle, pentose phosphate pathway, chlorophyll content, terpene emission, etc.) are modified, gene expression altered (calmodulin, calcium-dependent protein kinase, and proteinase inhibitor), and growth reduced (stem elongation and dry weight) after low power (i.e., nonthermal) HF-EMF exposure. These changes occur not only in the tissues directly exposed but also systemically in distant tissues. While the long-term impact of these metabolic changes remains largely unknown, we propose to consider nonionizing HF-EMF radiation as a noninjurious, genuine environmental factor that readily evokes changes in plant metabolism. PMID:26981524

  2. Plant Responses to High Frequency Electromagnetic Fields

    PubMed Central

    Vian, Alain; Davies, Eric; Gendraud, Michel; Bonnet, Pierre

    2016-01-01

    High frequency nonionizing electromagnetic fields (HF-EMF) that are increasingly present in the environment constitute a genuine environmental stimulus able to evoke specific responses in plants that share many similarities with those observed after a stressful treatment. Plants constitute an outstanding model to study such interactions since their architecture (high surface area to volume ratio) optimizes their interaction with the environment. In the present review, after identifying the main exposure devices (transverse and gigahertz electromagnetic cells, wave guide, and mode stirred reverberating chamber) and general physics laws that govern EMF interactions with plants, we illustrate some of the observed responses after exposure to HF-EMF at the cellular, molecular, and whole plant scale. Indeed, numerous metabolic activities (reactive oxygen species metabolism, α- and β-amylase, Krebs cycle, pentose phosphate pathway, chlorophyll content, terpene emission, etc.) are modified, gene expression altered (calmodulin, calcium-dependent protein kinase, and proteinase inhibitor), and growth reduced (stem elongation and dry weight) after low power (i.e., nonthermal) HF-EMF exposure. These changes occur not only in the tissues directly exposed but also systemically in distant tissues. While the long-term impact of these metabolic changes remains largely unknown, we propose to consider nonionizing HF-EMF radiation as a noninjurious, genuine environmental factor that readily evokes changes in plant metabolism. PMID:26981524

  3. Ecophysiology of Fe-Cycling Bacteria in Acidic Sediments ▿ †

    PubMed Central

    Lu, Shipeng; Gischkat, Stefan; Reiche, Marco; Akob, Denise M.; Hallberg, Kevin B.; Küsel, Kirsten

    2010-01-01

    Using a combination of cultivation-dependent and -independent methods, this study aimed to elucidate the diversity of microorganisms involved in iron cycling and to resolve their in situ functional links in sediments of an acidic lignite mine lake. Using six different media with pH values ranging from 2.5 to 4.3, 117 isolates were obtained that grouped into 38 different strains, including 27 putative new species with respect to the closest characterized strains. Among the isolated strains, 22 strains were able to oxidize Fe(II), 34 were able to reduce Fe(III) in schwertmannite, the dominant iron oxide in this lake, and 21 could do both. All isolates falling into the Gammaproteobacteria (an unknown Dyella-like genus and Acidithiobacillus-related strains) were obtained from the top acidic sediment zones (pH 2.8). Firmicutes strains (related to Bacillus and Alicyclobacillus) were only isolated from deep, moderately acidic sediment zones (pH 4 to 5). Of the Alphaproteobacteria, Acidocella-related strains were only isolated from acidic zones, whereas Acidiphilium-related strains were isolated from all sediment depths. Bacterial clone libraries generally supported and complemented these patterns. Geobacter-related clone sequences were only obtained from deep sediment zones, and Geobacter-specific quantitative PCR yielded 8 × 105 gene copy numbers. Isolates related to the Acidobacterium, Acidocella, and Alicyclobacillus genera and to the unknown Dyella-like genus showed a broad pH tolerance, ranging from 2.5 to 5.0, and preferred schwertmannite to goethite for Fe(III) reduction. This study highlighted the variety of acidophilic microorganisms that are responsible for iron cycling in acidic environments, extending the results of recent laboratory-based studies that showed this trait to be widespread among acidophiles. PMID:20971876

  4. Microbial Populations Involved in Cycling of Dimethyl Sulfide and Methanethiol in Freshwater Sediments

    PubMed Central

    Lomans, Bart P.; Luderer, Rianne; Steenbakkers, Peter; Pol, Arjan; van der Drift, Chris; Vogels, Godfried D.; Op den Camp, Huub J. M.

    2001-01-01

    Although several microorganisms that produce and degrade methanethiol (MT) and dimethyl sulfide (DMS) have been isolated from various habitats, little is known about the numbers of these microorganisms in situ. This study reports on the identification and quantification of microorganisms involved in the cycling of MT and DMS in freshwater sediments. Sediment incubation studies revealed that the formation of MT and DMS is well balanced with their degradation. MT formation depends on the concentrations of both sulfide and methyl group-donating compounds. A most-probable number (MPN) dilution series with syringate as the growth substrate showed that methylation of sulfide with methyl groups derived from syringate is a commonly occurring process in situ. MT appeared to be primarily degraded by obligately methylotrophic methanogens, which were found in the highest positive dilutions on DMS and mixed substrates (methanol, trimethylamine [TMA], and DMS). Amplified ribosomal DNA restriction analysis (ARDRA) and 16S rRNA gene sequence analysis of the total DNA isolated from the sediments and of the DNA isolated from the highest positive dilutions of the MPN series (mixed substrates) revealed that the methanogens that are responsible for the degradation of MT, DMS, methanol, and TMA in situ are all phylogenetically closely related to Methanomethylovorans hollandica. This was confirmed by sequence analysis of the product obtained from a nested PCR developed for the selective amplification of the 16S rRNA gene from M. hollandica. The data from sediment incubation experiments, MPN series, and molecular-genetics detection correlated well and provide convincing evidence for the suggested mechanisms for MT and DMS cycling and the common presence of the DMS-degrading methanogen M. hollandica in freshwater sediments. PMID:11229890

  5. Impacts of bioturbation on temporal variation in bacterial and archaeal nitrogen-cycling gene abundance in coastal sediments.

    PubMed

    Laverock, B; Tait, K; Gilbert, J A; Osborn, A M; Widdicombe, S

    2014-02-01

    In marine environments, macrofauna living in or on the sediment surface may alter the structure, diversity and function of benthic microbial communities. In particular, microbial nitrogen (N)-cycling processes may be enhanced by the activity of large bioturbating organisms. Here, we study the effect of the burrowing mud shrimp Upogebia deltaura upon temporal variation in the abundance of genes representing key N-cycling functional guilds. The abundance of bacterial genes representing different N-cycling guilds displayed different temporal patterns in burrow sediments in comparison with surface sediments, suggesting that the burrow provides a unique environment where bacterial gene abundances are influenced directly by macrofaunal activity. In contrast, the abundances of archaeal ammonia oxidizers varied temporally but were not affected by bioturbation, indicating differential responses between bacterial and archaeal ammonia oxidizers to environmental physicochemical controls. This study highlights the importance of bioturbation as a control over the temporal variation in nitrogen-cycling microbial community dynamics within coastal sediments. PMID:24596269

  6. Impacts of bioturbation on temporal variation in bacterial and archaeal nitrogen-cycling gene abundance in coastal sediments

    PubMed Central

    Laverock, B; Tait, K; Gilbert, J A; Osborn, A M; Widdicombe, S

    2014-01-01

    In marine environments, macrofauna living in or on the sediment surface may alter the structure, diversity and function of benthic microbial communities. In particular, microbial nitrogen (N)-cycling processes may be enhanced by the activity of large bioturbating organisms. Here, we study the effect of the burrowing mud shrimp Upogebia deltaura upon temporal variation in the abundance of genes representing key N-cycling functional guilds. The abundance of bacterial genes representing different N-cycling guilds displayed different temporal patterns in burrow sediments in comparison with surface sediments, suggesting that the burrow provides a unique environment where bacterial gene abundances are influenced directly by macrofaunal activity. In contrast, the abundances of archaeal ammonia oxidizers varied temporally but were not affected by bioturbation, indicating differential responses between bacterial and archaeal ammonia oxidizers to environmental physicochemical controls. This study highlights the importance of bioturbation as a control over the temporal variation in nitrogen-cycling microbial community dynamics within coastal sediments. PMID:24596269

  7. Source of the Magnetic Susceptibility Variations in Southern Ocean Sediments Over the Last Glacial Cycle

    NASA Astrophysics Data System (ADS)

    Maher, B.; Thompson, R.

    2014-12-01

    Changes in the sources, mineralogy and rates of iron supply to the Southern Ocean may have global impact and significance, by influencing plankton growth rates and nutrient take-up in this, the largest of the high nutrient low chlorophyll (HNLC) regions of the world ocean. Iron 'fertilization' in the Southern Ocean may increase rates of carbon export production and thus the ocean uptake flux of atmospheric CO2, and also diminish the northward flow of residual nutrients to the extra-polar ocean, especially the HNLC regions of the Pacific. Changes in Southern Ocean export production may contribute to global climate change over glacial-interglacial timescales. The key sources of iron for the Southern Ocean are reported to be windblown dust and sedimentary supply; their relative significance an issue of much long-standing debate. Links between aeolian dust fluxes to the Southern Ocean and to the Antarctic ice cores have been proposed for the Scotia Sea region of the Southern Ocean, downwind from the South American land mass. Regional downcore variations in the magnetic susceptibility of sediments from the Scotia Sea show remarkable similarity to variations in dust concentration and flux in East Antarctic ice cores (with glacial stages characterised by increases in ice dust and sediment magnetic susceptibility). Indeed, the strength of the ice dust/sediment magnetism correlations (r ~ 0.7) provides a pragmatic basis for use of the sedimentary magnetic susceptibility records as a chronostratigraphic proxy, a boon in the carbonate-free deep-sea sediments of the Southern Ocean. However, the source and causal basis of the sediment magnetism/ice dust co-variations remain controversial; aeolian dust, bacterial magnetite and wind-driven current transport of marine sediment have all been invoked as possible key sources. Here, we use magnetic and isotopic methods to resolve this debate, and identify and quantify the sources of magnetic material to the Scotia Sea for the last

  8. Natural Organobromine in Marine Sediments: New Evidence of Biogeochemical Br Cycling

    SciTech Connect

    A Leri; J Hakala; M Marcus; A Lanzirotti; C Reddy; S Myneni

    2011-12-31

    Organobromine (Br{sub org}) compounds, commonly recognized as persistent, toxic anthropogenic pollutants, are also produced naturally in terrestrial and marine systems. Several enzymatic and abiotic bromination mechanisms have been identified, as well as an array of natural Br{sub org} molecules associated with various marine organisms. The fate of the carbon-bromine functionality in the marine environment, however, remains largely unexplored. Oceanographic studies have noted an association between bromine (Br) and organic carbon (C{sub org}) in marine sediments. Even so, there has been no direct chemical evidence that Br in the sediments exists in a stable form apart from inorganic bromide (Br{sub inorg}), which is widely presumed conservative in marine systems. To investigate the scope of natural Br{sub org} production and its fate in the environment, we probed Br distribution and speciation in estuarine and marine sediments using in situ X-ray spectroscopy and spectromicroscopy. We show that Br{sub org} is ubiquitous throughout diverse sedimentary environments, occurring in correlation with C{sub org} and metals such as Fe, Ca, and Zn. Analysis of sinking particulate carbon from the seawater column links the Br{sub org} observed in sediments to biologically produced Br{sub org} compounds that persist through humification of natural organic matter (NOM). Br speciation varies with sediment depth, revealing biogeochemical cycling of Br between organic and inorganic forms as part of the burial and degradation of NOM. These findings illuminate the chemistry behind the association of Br with Corg in marine sediments and cast doubt on the paradigmatic classification of Br as a conservative element in seawater systems.

  9. Iron Cycling in Marine Sediments - New Insights from Isotope Analysis on Sequentially Extracted Fe Fractions

    NASA Astrophysics Data System (ADS)

    Henkel, S.; Kasten, S.; Poulton, S.; Hartmann, J.; Staubwasser, M.

    2014-12-01

    Reactive Fe (oxyhydr)oxides preferentially undergo early diagenetic cycling and may cause a diffusive flux of dissolved Fe2+ from sediments towards the sediment-water interface. The partitioning of Fe in sediments has traditionally been studied by applying sequential extractions based on reductive dissolution of Fe minerals. We complemented the sequential leaching method by Poulton and Canfield [1] in order to be able to gain δ56Fe data for specific Fe fractions, as such data are potentially useful to study Fe cycling in marine environments. The specific mineral fractions are Fe-carbonates, ferrihydrite + lepidocrocite, goethite + hematite, and magnetite. Leaching was performed with acetic acid, hydroxylamine-HCl, Na-dithionite and oxalic acid. The processing of leachates for δ56Fe analysis involved boiling the samples in HCl/HNO3/H2O2, Fe precipitation and anion exchange column chromatography. The new method was applied to short sediment cores from the North Sea and a bay of King George Island (South Shetland Islands, Antarctica). Downcore mineral-specific variations in δ56Fe revealed differing contributions of Fe (oxyhydr)oxides to redox cycling. A slight decrease in easily reducible Fe oxides correlating with a slight increase in δ56Fe for this fraction with depth, which is in line with progessive dissimilatory iron reduction [2,3], is visible in the top 10 cm of the North Sea core, but not in the antarctic sediments. Less reactive (dithionite and oxalate leachable) fractions did not reveal isotopic trends. The acetic acid-soluble fraction displayed pronounced δ56Fe trends at both sites that cannot be explained by acid volatile sulfides that are also extracted by acetic acid [1]. We suggest that low δ56Fe values in this fraction relative to the pool of easily reducible Fe oxides result from adsorbed Fe(II) that was open to isotopic exchange with oxide surfaces, affirming the experimental results of Crosby el al. [2]. Hence, δ56Fe analyses on marine

  10. Mechanisms for high-frequency cyclicity in the Upper Jurassic limestone of northeastern Mexico

    SciTech Connect

    Johnson, C.R.; Ward, W.C. ); Goldhammer, R.K. )

    1991-03-01

    The 520 m of Upper Jurassic Zuloaga Limestone exposed in the Sierra de Bunuelos in southern Coahuila comprise 118 cycles of peritidal carbonate rock deposited on a gently dipping ramp. Field studies with Fischer plots and time-series analysis suggest that a Milankovitchian glacioeustasy mechanism is inadequate to describe the Zuloaga cycles. Autocyclic progradation may have been the major influence on depositional cyclicity. Depositional cycles in the Zuloaga Formation typically are a few meters thick and asymmetric with subtidal wackestone and packstone grading upward into subtidal grainstone or into intertidal stromatolites. Width of the carbonate ramp is estimated to have been about 150 km. Sedimentation rates for these peritidal carbonate environments apparently exceeded subsidence rates inasmuch as most of the carbonate platform remained near sea level during Zuloaga deposition. The area was tectonically quiescent during the late Jurassic. Autocyclic shoreline progradation is a feasible mechanism for producing the high-frequency cycles, as suggested by (1) poor correlation with predicted Milankovitch periodicity shown by time-series analysis, (2) little evidence of subaerial exposure, (3) development of complete peritidal cycles, (4) general progradational sequences within each third-order unit, and (5) absence of polar glaciation during Late Jurassic.

  11. High frequency oscillations in the intact brain

    PubMed Central

    Buzsáki, György; da Silva, Fernando Lopes

    2016-01-01

    High frequency oscillations (HFOs) constitute a novel trend in neurophysiology that is fascinating neuroscientists in general, and epileptologists in particular. But what are HFOs? What is the frequency range of HFOs? Are there different types of HFOs, physiological and pathological? How are HFOs generated? Can HFOs represent temporal codes for cognitive processes? These questions are pressing and this symposium volume attempts to give constructive answers. As a prelude to this exciting discussion, we summarize the physiological high frequency patterns in the intact brain, concentrating mainly on hippocampal patterns, where the mechanisms of high frequency oscillations are perhaps best understood. PMID:22449727

  12. Genomic reconstruction of novel sediment phyla enlightens roles in sedimentary biogeochemical cycling

    NASA Astrophysics Data System (ADS)

    Baker, B.; Lazar, C.; Seitz, K.; Teske, A.; Hinrichs, K. U.; Dick, G.

    2015-12-01

    Estuaries are among the most productive habitats on the planet. Microbes in estuary sediments control the turnover of organic carbon, and the anaerobic cycling of nitrogen and sulfur. These communities are complex and primarily made up of uncultured lineages, thus little is known about how ecological and metabolic processes are partitioned in sediments. We reconstructed 82 bacterial and 24 archaeal high-quality genomes from different redox regimes (sulfate-rich, sulfate-methane transition zone, and methane-rich zones) of estuary sediments. These bacteria belong to 23 distinct groups, including uncultured candidate phyla (eg. KSB1, TA06, and KD3-62), and three newly described phyla (WOR-1, and -2, and -3). The archaea encompass 8 widespread sediment lineages including MGB-D, RC-III and IV, Z7ME43, Parvarchaeota, Lokiarchoaeta (MBG-B), SAGMEG, Bathyarchaeota (groups MCG-1, -6, -7, and -15) and previously unrecognized deeply branched phylum "Thorarchaeota". The uncultured phyla mediate essential biogeochemical processes of the estuarine environment. Z7ME43 archaea have genes for S disproportionation (S0 reduction and thiosulfate reduction and oxidation). SAGMEG appear to be strict anaerobes capable of coupling CO/H2 oxidation to either S0 or nitrite reduction and have novel RubisCO genes for carbon fixation. Thorarchaeota contain pathways for acetate production from the degradation of detrital proteins and intermediate S cycling. Furthermore, the gene content of this group revealed links in the evolutionary histories of archaea and eukaryotes. This dataset extents our knowledge of the metabolic potential of several uncultured phyla. We were able to chart the flow of carbon and nutrients through the multiple layers of bacterial processing and reveal potential ecological interactions within the communities.

  13. Self-stressing test structures used for high-frequency electromigration

    SciTech Connect

    Snyder, E.S.; Pierce, D.G.; Campbell, D.V.; Swanson, S.E.

    1994-02-01

    We demonstrate for the first time high frequency (500 mhz) electromigration at the wafer-level using on-chip, self-stressing test structures. Since the stress temperature, frequency, duty cycle and current are controlled by DC signals in these structures, we used conventional DC test equipment without any special modifications (such as high frequency cabling, high temperature probe cards, etc.). This structure significantly reduces the cost of performing realistic high frequency electromigration experiments.

  14. Oxygen, a regulating factor for nitrogen cycling in continental shelf sediments?

    NASA Astrophysics Data System (ADS)

    Neubacher, E.; Parker, R.; Trimmer, M.

    2009-04-01

    Oxygen is a fundamental parameter in regulating the cycling of carbon and nitrogen in continental shelf seas. Recent studies and models have shown that some regions of coastal seas are prone to a lack of oxygen and under such conditions, the dynamics of the nitrogen cycle could be altered. We measured rates of denitrification, anaerobic ammonium oxidation (anammox), oxygen uptake, nutrient exchange and pore water profiles of oxygen in sediments of the southern North Sea experimentally exposed to different oxygen saturations. The incubation of sediment at 33% (of air-saturation) for oxygen reduced the penetration and consumption of oxygen by the sediment by of approximately 50%, with a new steady state being reached after approximately 75 min. The rates of the various processes showed strong seasonality over the survey period (2007-2008), with denitrification ranging from 0.6 to 21.2 mol N m-2 h-1, anammox 0.2 to 4.4 mol N m-2 h-1 and oxygen uptake 46.5 to 631.8 mol O2 m-2 h-1. At the reduced saturation for oxygen (~ 33%), denitrification increased significantly (30%) while anammox remained constant. On average anammox accounted for 14-28% of the total production of di-nitrogen (N2) gas. Under ambient oxygen saturation, the rates of oxygen uptake and total production of N2 were positively correlated with each other (r = 0.73, p = 0.01, n = 230). The increase in denitrification was coupled to a change in NO3- flux. Under ambient oxygen there was a net release of 10.5 mol NO3- m-2 h-1 from the sediment to the water column, whereas under reduced oxygen, the sediments became a sink for NO3- (-1.2 mol NO3- m-2 h-1) from the overlaying water. These results suggest that facultative denitrifying bacteria were able to exploit the newly extended suboxic sediment layer, while the monophyletic group of anammox bacteria were not as flexible.

  15. A microbial arsenic cycle in sediments of an acidic mine impoundment: Herman Pit, Clear Lake, California

    USGS Publications Warehouse

    Blum, Jodi S.; McCann, Shelley; Bennett, S.; Miller, Laurence G.; Stolz, J. R.; Stoneburner, B.; Saltikov, C.; Oremland, Ronald S.

    2015-01-01

    The involvement of prokaryotes in the redox reactions of arsenic occurring between its +5 [arsenate; As(V)] and +3 [arsenite; As(III)] oxidation states has been well established. Most research to date has focused upon circum-neutral pH environments (e.g., freshwater or estuarine sediments) or arsenic-rich “extreme” environments like hot springs and soda lakes. In contrast, relatively little work has been conducted in acidic environments. With this in mind we conducted experiments with sediments taken from the Herman Pit, an acid mine drainage impoundment of a former mercury (cinnabar) mine. Due to the large adsorptive capacity of the abundant Fe(III)-rich minerals, we were unable to initially detect in solution either As(V) or As(III) added to the aqueous phase of live sediment slurries or autoclaved controls, although the former consumed added electron donors (i.e., lactate, acetate, hydrogen), while the latter did not. This prompted us to conduct further experiments with diluted slurries using the live materials from the first incubation as inoculum. In these experiments we observed reduction of As(V) to As(III) under anoxic conditions and reduction rates were enhanced by addition of electron donors. We also observed oxidation of As(III) to As(V) in oxic slurries as well as in anoxic slurries amended with nitrate. We noted an acid-tolerant trend for sediment slurries in the cases of As(III) oxidation (aerobic and anaerobic) as well as for anaerobic As(V) reduction. These observations indicate the presence of a viable microbial arsenic redox cycle in the sediments of this extreme environment, a result reinforced by the successful amplification of arsenic functional genes (aioA, and arrA) from these materials.

  16. Nitrite isotope dynamics in coastal sediments: An intricate link between nitrogen and oxygen cycling

    NASA Astrophysics Data System (ADS)

    Charoenpong, C.; Buchwald, C.; Ziebis, W.; Wankel, S. D.

    2014-12-01

    Marine sediments often exhibit strong redox gradients, hosting a range of important nitrogen transformation processes. While the interplay among these microbially catalyzed nitrogen transformations has been well studied in the water column, the sharp redox transition in sediments often makes it far more difficult to unravel the complexity underpinning the cycling of nitrogen. Although often low in concentration, nitrite represents an important 'crossroad' in the nitrogen cycle as a reactive intermediate of both reductive and oxidative N transformations, including nitrification, dissimilatory nitrate reduction to ammonium and denitrification. Here we focus on the dual isotopic composition of nitrite (δ15N and δ18O), in concert with nitrate and ammonium data, as a means for constraining the sedimentary N cycling. Intact flow-through core incubations were performed on sediments collected from intertidal flats on the island of Sylt, Germany. Three types of substrate (i.e., sand, silt, and clay) were collected and subjected to different oxygen (i.e., ambient vs depleted) and nitrogen (i.e., ambient vs highly loaded) regimes. In addition to the measurement of natural abundance N and O stable isotopes, we also amended cores with nitrate having a positive ∆17O in our high nitrogen treatment, which offers yet an additional tracer to further constrain these transformations. While the concentration and isotopic composition (δ15N and δ18O) of nitrite act to integrate the influence of major N redox reactions, the N and O isotope systematics are decoupled. Although nitrogen atoms are generally conserved among these transformations, oxygen isotopes of nitrite are subject to a different set of processes. For example, the loss of an oxygen atom during the reductive processes of NO3- and NO2- reduction, the gain of oxygen atoms from O2 and water during nitrification, and oxygen isotopic equilibration between nitrite and water are all reflected in the δ18O of NO2-. Thus, the

  17. Frequency shifts of high frequency p-modes

    NASA Technical Reports Server (NTRS)

    Jain, Rekha

    1995-01-01

    Frequency shifts of high frequency p-modes during the solar cycle are calculated for a non-magnetic polytrope convection zone model. An isothermal chromospheric atmosphere threaded by a uniform horizontal magnetic field is correlated to this model. The relevant observations of such frequency changes are discussed. The calculated simultaneous changes in the field strength and chromospheric temperature result in the frequency shifts that are similar to those of the observations.

  18. Nitrogen cycling processes and microbial community composition in bed sediments in the Yukon River at Pilot Station

    NASA Astrophysics Data System (ADS)

    Repert, Deborah A.; Underwood, Jennifer C.; Smith, Richard L.; Song, Bongkeun

    2014-12-01

    Information on the contribution of nitrogen (N)-cycling processes in bed sediments to river nutrient fluxes in large northern latitude river systems is limited. This study examined the relationship between N-cycling processes in bed sediments and N speciation and loading in the Yukon River near its mouth at the Bering Sea. We conducted laboratory bioassays to measure N-cycling processes in sediment samples collected over distinct water cycle seasons. In conjunction, the microbial community composition in the bed sediments using genes involved in N-cycling (narG, napA, nosZ, and amoA) and 16S rRNA gene pyrosequences was examined. Temporal variation was observed in net N mineralization, nitrate uptake, and denitrification rate potentials and correlated strongly with sediment carbon (C) and extractable N content and microbial community composition rather than with river water nutrient concentrations. The C content of the bed sediment was notably impacted by the spring flood, ranging from 1.1% in the midst of an ice-jam to 0.1% immediately after ice-out, suggesting a buildup of organic material (OM) prior to scouring of the bed sediments during ice break up. The dominant members of the microbial community that explained differences in N-processing rates belonged to the genera Crenothrix, Flavobacterium, and the family of Comamonadaceae. Our results suggest that biogeochemical processing rates in the bed sediments appear to be more coupled to hydrology, nutrient availability in the sediments, and microbial community composition rather than river nutrient concentrations at Pilot Station.

  19. Nitrogen cycling processes and microbial community composition in bed sediments in the Yukon River at Pilot Station

    USGS Publications Warehouse

    Repert, Deborah A.; Underwood, Jennifer C.; Smith, Richard L.; Song, Bongkeun

    2014-01-01

    Information on the contribution of nitrogen (N)-cycling processes in bed sediments to river nutrient fluxes in large northern latitude river systems is limited. This study examined the relationship between N-cycling processes in bed sediments and N speciation and loading in the Yukon River near its mouth at the Bering Sea. We conducted laboratory bioassays to measure N-cycling processes in sediment samples collected over distinct water cycle seasons. In conjunction, the microbial community composition in the bed sediments using genes involved in N-cycling (narG, napA, nosZ, and amoA) and 16S rRNA gene pyrosequences was examined. Temporal variation was observed in net N mineralization, nitrate uptake, and denitrification rate potentials and correlated strongly with sediment carbon (C) and extractable N content and microbial community composition rather than with river water nutrient concentrations. The C content of the bed sediment was notably impacted by the spring flood, ranging from 1.1% in the midst of an ice-jam to 0.1% immediately after ice-out, suggesting a buildup of organic material (OM) prior to scouring of the bed sediments during ice break up. The dominant members of the microbial community that explained differences in N-processing rates belonged to the genera Crenothrix,Flavobacterium, and the family of Comamonadaceae. Our results suggest that biogeochemical processing rates in the bed sediments appear to be more coupled to hydrology, nutrient availability in the sediments, and microbial community composition rather than river nutrient concentrations at Pilot Station.

  20. Sediment trapping in the Changjiang Estuary: Observations in the North Passage over a spring-neap tidal cycle

    NASA Astrophysics Data System (ADS)

    Li, Xiangyu; Zhu, Jianrong; Yuan, Rui; Qiu, Cheng; Wu, Hui

    2016-08-01

    Water current, salinity, and suspended sediment concentration (SSC) were measured at three anchored boat sites along the North Passage (NP) of the Changjiang Estuary over a spring-neap tidal cycle, in order to study sediment trapping and siltation in the estuary. Pronounced stratification was observed during the late flood tide and the following early ebb tide, along with an advancing and retreating salt wedge, whereas strong vertical mixing occurred during the late ebb when the effect of the salt wedge faded. Therefore, the SSC in the flood-ebb tidal cycle tended to be asymmetric. In the upper reach of the NP, the seaward advective near-bed sediment transport dominated the total near-bed sediment transport, whereas in the middle reach of the NP, the landward tidal pumping component dominated. Accordingly, a notable convergent near-bed residual sediment transport was generated near the middle reach. Because the convergence of residual sediment transport in the region of a salt wedge is generally recognized as sediment trapping, convergent near-bed residual sediment transport is the cause of the high sedimentation rate in the NP.

  1. Real-time, high frequency QRS electrocardiograph

    NASA Technical Reports Server (NTRS)

    Schlegel, Todd T. (Inventor); DePalma, Jude L. (Inventor); Moradi, Saeed (Inventor)

    2006-01-01

    Real time cardiac electrical data are received from a patient, manipulated to determine various useful aspects of the ECG signal, and displayed in real time in a useful form on a computer screen or monitor. The monitor displays the high frequency data from the QRS complex in units of microvolts, juxtaposed with a display of conventional ECG data in units of millivolts or microvolts. The high frequency data are analyzed for their root mean square (RMS) voltage values and the discrete RMS values and related parameters are displayed in real time. The high frequency data from the QRS complex are analyzed with imbedded algorithms to determine the presence or absence of reduced amplitude zones, referred to herein as RAZs. RAZs are displayed as go, no-go signals on the computer monitor. The RMS and related values of the high frequency components are displayed as time varying signals, and the presence or absence of RAZs may be similarly displayed over time.

  2. Overview of the Advanced High Frequency Branch

    NASA Technical Reports Server (NTRS)

    Miranda, Felix A.

    2015-01-01

    This presentation provides an overview of the competencies, selected areas of research and technology development activities, and current external collaborative efforts of the NASA Glenn Research Center's Advanced High Frequency Branch.

  3. High frequency testing of rubber mounts.

    PubMed

    Vahdati, Nader; Saunders, L Ken Lauderbaugh

    2002-04-01

    Rubber and fluid-filled rubber engine mounts are commonly used in automotive and aerospace applications to provide reduced cabin noise and vibration, and/or motion accommodations. In certain applications, the rubber mount may operate at frequencies as high as 5000 Hz. Therefore, dynamic stiffness of the mount needs to be known in this frequency range. Commercial high frequency test machines are practically nonexistent, and the best high frequency test machine on the market is only capable of frequencies as high as 1000 Hz. In this paper, a high frequency test machine is described that allows test engineers to study the high frequency performance of rubber mounts at frequencies up to 5000 Hz. PMID:12071247

  4. High frequency stimulation can block axonal conduction.

    PubMed

    Jensen, Alicia L; Durand, Dominique M

    2009-11-01

    High frequency stimulation (HFS) is used to control abnormal neuronal activity associated with movement, seizure, and psychiatric disorders. Yet, the mechanisms of its therapeutic action are not known. Although experimental results have shown that HFS suppresses somatic activity, other data has suggested that HFS could generate excitation of axons. Moreover it is unclear what effect the stimulation has on tissue surrounding the stimulation electrode. Electrophysiological and computational modeling literature suggests that HFS can drive axons at the stimulus frequency. Therefore, we tested the hypothesis that unlike cell bodies, axons are driven by pulse train HFS. This hypothesis was tested in fibers of the hippocampus both in-vivo and in-vitro. Our results indicate that although electrical stimulation could activate and drive axons at low frequencies (0.5-25 Hz), as the stimulus frequency increased, electrical stimulation failed to continuously excite axonal activity. Fiber tracts were unable to follow extracellular pulse trains above 50 Hz in-vitro and above 125 Hz in-vivo. The number of cycles required for failure was frequency dependent but independent of stimulus amplitude. A novel in-vitro preparation was developed, in which, the alveus was isolated from the remainder of the hippocampus slice. The isolated fiber tract was unable to follow pulse trains above 75 Hz. Reversible conduction block occurred at much higher stimulus amplitudes, with pulse train HFS (>150 Hz) preventing propagation through the site of stimulation. This study shows that pulse train HFS affects axonal activity by: (1) disrupting HFS evoked excitation leading to partial conduction block of activity through the site of HFS; and (2) generating complete conduction block of secondary evoked activity, as HFS amplitude is increased. These results are relevant for the interpretation of the effects of HFS for the control of abnormal neural activity such as epilepsy and Parkinson's disease. PMID

  5. Microbial cycling of mercury in contaminated pelagic and wetland sediments of San Pablo Bay, California

    USGS Publications Warehouse

    Marvin-DiPasquale, M. C.; Agee, J.L.; Bouse, R.M.; Jaffe, B.E.

    2003-01-01

    San Pablo Bay is an estuary, within northern San Francisco Bay, containing elevated sediment mercury (Hg) levels because of historic loading of hydraulic mining debris during the California gold-rush of the late 1800s. A preliminary investigation of benthic microbial Hg cycling was conducted in surface sediment (0-4 cm) collected from one salt-marsh and three open-water sites. A deeper profile (0-26 cm) was evaluated at one of the open-water locations. Radiolabeled model Hg-compounds were used to measure rates of both methylmercury (MeHg) production and degradation by bacteria. While all sites and depths had similar total-Hg concentrations (0.3-0.6 ppm), and geochemical signatures of mining debris (as eNd, range: -3.08 to -4.37), in-situ MeHg was highest in the marsh (5.4??3.5 ppb) and ??? 0.7 ppb in all open-water sites. Microbial MeHg production (potential rate) in 0-4 surface sediments was also highest in the marsh (3.1 ng g-1 wet sediment day-1) and below detection (<0.06 ng g-1 wet sediment day-1) in open-water locations. The marsh exhibited a methylation/demethylation (M/D) ratio more than 25x that of all open-water locations. Only below the surface 0-4-cm horizon was significant MeHg production potential evident in the open-water sediment profile (0.2-1.1 ng g-1 wet sediment day-1). In-situ Hg methylation rates, calculated from radiotracer rate constants, and in-situ inorganic Hg(II) concentrations compared well with potential rates. However, similarly calculated in-situ rates of MeHg degradation were much lower than potential rates. These preliminary data indicate that wetlands surrounding San Pablo Bay represent important zones of MeHg production, more so than similarly Hg-contaminated adjacent open-water areas. This has significant implications for this and other Hg-impacted systems, where wetland expansion is currently planned.

  6. Magnetic Properties of Bermuda Rise Sediments Controlled by Glacial Cycles During the Late Pleistocene

    NASA Astrophysics Data System (ADS)

    Roud, S.

    2015-12-01

    Sediments from ODP site 1063 (Bermuda Rise, North Atlantic) contain a high-resolution record of geomagnetic field behavior during the Brunhes Chron. We present rock magnetic data of the upper 160 mcd (<900 ka) from hole 1063D that show magnetic properties vary in concert with glacial cycles. Magnetite appears to be the main magnetic carrier in the carbonate-dominated interglacial horizons, yet exhibits contrasting grain size distributions depending on the redox state of the horizons. Higher contributions of single domain magnetite exist above the present day sulfate reduction zone (ca. 44 mcd) with relatively higher multidomain magnetite components below that likely arise from the partial dissolution of SD magnetite in the deeper, anoxic horizons. Glacial horizons on the other hand, characterized by enhanced terrigenous deposition, show no evidence for diagenetic dissolution but do indicate the presence of authigenic greigite close to glacial maxima (acquisition of gyro-remanence, strong magnetostatic interactions and SD properties). Glacial horizons contain hematite (maxima in HIRM and S-Ratio consistent with a reddish hue) and exhibit higher ARM anisotropy and pronounced sedimentary fabrics. We infer that post depositional processes affected the magnetic grain size and mineralogy of Bermuda rise sediments deposited during the late Pleistocene. Hematite concentration is interpreted to reflect primary terrigenous input that is likely derived from the Canadian Maritime Provinces. A close correlation between HIRM and magnetic foliation suggests that changes in sediment composition (terrigenous vs. marine biogenic) were accompanied by changes in the depositional processes at the site.

  7. Sulfur and iron cycling in deep-subsurface, coal bed-containing sediments off Shimokita (Japan)

    NASA Astrophysics Data System (ADS)

    Riedinger, N.; Smirnoff, M. N.; Gilhooly, W.; Phillips, S. C.; Lyons, T. W.; 337 Scientific Party, I.

    2013-12-01

    The main goal of IODP Expedition 337 was the identification and characterization of the deep coal bed biosphere and hydrocarbon system off the Shimokita Peninsula (Japan) in the northwestern Pacific using the D/V Chikyu. To accomplish this scientific objective, it was also necessary to investigate the inorganic biogeochemistry in order to identify possible electron acceptors and bio-essential nutrients. These biogeochemical parameters greatly influence both, the composition and abundance of microbial communities as well as the organic carbon cycle. In turn, the microbially mediated carbon cycle influences the diagenetic reactions in the subsurface, thus, altering geochemical and physical characteristics of the material. Here we present results from metal and sulfur geochemical analyses from the deep-subsurface sediments (about 1250 to 2466 mbsf) at Site C0020 off Shimokita. The measured concentrations of acid volatile sulfur (AVS) as well as chromium reducible sulfur (CRS) reflect the alteration of iron oxides to iron sulfides and indicate that the main sulfur-bearing phase in the investigated sediments is pyrite. Concentrations of intermediate sulfur species are minor and occur mainly in the coal-bearing interval. Our data show that the uppermost sediments contain higher amounts of pyrite (up to 1.2 wt.%) with an average of 0.5 wt.% compared to the deeper deposits (below about 1800 mbsf), which show an average of 0.16 wt.%. In contrast, iron oxide concentrations are highest in the deeper sediment sections (up to 0.4%), where pyrite concentrations are low. The alteration of iron oxides to sulfides in theses lower section was probably governed by the amount of available sulfide in the pore water. The occurrence of (bio-)reactive iron phases in these deeply buried sediments has implications for the deep biosphere as those minerals have the potential to serve as electron acceptors during burial, including reactions involving deep sourced electron donors, such as

  8. Biogeography and diversity of methane and sulfur-cycling ecotypes in deep subsurface sediments

    NASA Astrophysics Data System (ADS)

    Adams, M. M.; Biddle, J.; Girguis, P. R.

    2013-12-01

    The microbially mediated anaerobic oxidation of methane (AOM) is critical for regulating the flux of methane from the ocean. AOM is coupled to sulfate availability in many anoxic marine environments, which has been extensively studied at cold seeps, hydrothermal vents, and the sulfate-methane transition zone at the seafloor. The microbes known to catalyze AOM form phylogenetically distinct anaerobic methanotroph (ANME) clusters and sometimes live in concert with sulfate-reducing bacteria (SRB). Strikingly, certain ANME groups and subgroups have been shown to occupy different ecological niches in both hydrocarbon seep and hydrothermal vent sediments. However, the environmental parameters that select for certain phylogenetic variants or 'ecotypes' in a wide range of marine systems are still unknown. A marine environment that remains elusive to characterization of potential ANME and SRB ecotype diversity is methane hydrate formations in the deep subsurface. Current estimates indicate that seafloor hydrates may exceed 10,000 GtC at standard temperature and pressure conditions. However, only a handful of studies have investigated the potential for AOM in the deep subsurface associated with methane hydrates. To gain a better understanding of the distribution of methane- and sulfur- cycling ecotypes in biogeochemically distinct marine subsurface ecosystems, we generated a substantial library of 16S rRNA gene sequences for these uncultivable deep sea microorganisms using Illumina sequencing. Sediment strata were collected from the methane-hydrate associated deep subsurface of Hydrate Ridge (30 - 100 mbsf), hydrocarbon cold seeps of Monterey Bay, metalliferous sedimented hydrothermal vents of Juan de Fuca Ridge, and organic-rich hydrothermally influenced sediments of Guaymas Basin. We used the Illumina MiSeq sequencing platform to assess Archaeal and Bacterial richness in a total of 36 deep sea sediment samples followed by qPCR for quantification of ANME and SRB phylotype

  9. The effects of wastewater discharge on the microbiological nitrogen cycle of the lake sediments

    NASA Astrophysics Data System (ADS)

    Saarenheimo, Jatta; Aalto, Sanni L.; Tiirola, Marja

    2016-04-01

    Anthropogenic wastewater inputs alter the natural dynamics of nitrogen (N) cycle by providing high concentrations of nitrate and organic matter to the sediment microbes. It can also change the microbial community composition and N removal potential but this is currently not that well studied. To study these aspects, we conducted ecosystem-scale experiment in Lake Keurusselkä, Finland. In the experiment, the wastewater discharge to the recipient lake was optimized with sediment filtration, which increased the surface and retention time of the nitrified wastewater with the sediment. In addition to N transformation rates, which showed that optimization enhanced denitrification, we studied the microbial responses at the sediment. Genetic potential of nitrogen transformation processes, such as denitrification, dissimilatory nitrate reduction to ammonium (DNRA) and nitrification were studied by targeting the functional genes (i.e. nirS, nirK, nosZI, nosZII, nrfA, amoAarchaea and amoAbacteria) with quantitative PCR and digital droplet PCR. In addition, changes in the microbial community composition along the wastewater gradient were examined by using next generation sequencing of the 16S rRNA genes. In line with our hypothesis, the relative abundance of denitrifying genes followed the observed denitrification rates, being highest near the nitrate-rich wastewater discharge. Furthermore the microbial community composition in the discharge point differed clearly from the control and downstream sites, having also the highest numbers of rare OTUs. Abundance of nitrifying bacteria was higher than nitrifying archaea near the waste water discharge, whereas the opposite was seen at the control site. The results indicate that wastewater is not only increasing the denitrification rates, but can also alter the structure and genetic potential microbial communities.

  10. Time-dependent behavior of a placed bed of cohesive sediment subjected to erosion and deposition cycles

    NASA Astrophysics Data System (ADS)

    Mariotti, G.; Valentine, K.; Fagherazzi, S.

    2015-02-01

    This study aims to explore the behavior of a cohesive sediment bed that undergoes cycles of erosion and deposition under diluted conditions. A bed of bentonite (montmorillonite) sediment was placed in two annular flumes and subjected to daily erosion tests for a period of 80 days, mimicking intermittent moderate-energy disturbances like tidal currents and wind waves. After each erosion test, the suspended sediment was allowed to settle back in the flumes. The amount of suspended sediment measured at the top of the water column at the end of each erosion test decreased in the first 5 days, concurrently with an increase in the bulk-settling velocity near the bed. This pattern is explained by turbulence-induced flocculation of clay particles and consequent formation of a surface floc layer. After about 20 days, the amount of suspended sediment measured at the top of the water column at the end of each erosion test increased and the settling velocity decreased, whereas the suspended sediment concentration measured near the bed remained nearly constant. We explain such trend by the cumulative release of slow-settling particles from the bed. This experiment suggests that the superficial layer of a placed bed that is periodically eroded and redeposited experiences competing processes: the sediment that is resuspended at every cycle becomes less erodible, but prolonged exposure to shear stress increases the pool of eroded sediment over time. The total amount of resuspended sediment seems to become constant after several tens of cycle, suggesting that the release of particles from the bed by cumulative erosion is balanced by the binding of such particles to the bed.

  11. Life Cycle Sustainability Assessment of Sediment Remediation at the London Olympic Park

    NASA Astrophysics Data System (ADS)

    Hou, D.; Al-Tabbaa, A.

    2013-12-01

    In recent years, there is an emerging 'green and sustainable remediation' (GSR) movement. It is drawing increasing attention from both the government and the industry, because this GSR movement is promising in accelerating process in addressing the contaminated land issue, by overcoming regulatory barriers, encouraging technological innovation, and balancing life cycle environmental stewardship with economic vitality and social well-being. Life cycle assessment (LCA) has been increasingly used by both researchers and industrial practitioners in an initiative to make environmental remediation greener and more sustainable. Life cycle sustainability assessment (LCSA), aiming at expanding the traditional LCA model in both breadth and depth (e.g. to incorporate both environmental and social-economic sustainability), is an important research direction in the existing LCA research field. The present study intends to develop a LCSA method based on a hybrid LCA model and economic input-output (EIO) data. The LCSA method is applied to a contaminated sediment remediation project conducted at the London Olympic Park site.

  12. A geomorphic-geochemical framework for quantifying the cycling of sediment-associated contaminants in fluvial systems

    NASA Astrophysics Data System (ADS)

    Byrne, Patrick; Lopez-Tarazon, Jose; Williams, Richard

    2016-04-01

    Recent high-profile contamination events linked to extreme floods have underlined the persistent environmental risk posed by legacy metals stored in fluvial systems worldwide. While we understand that the fate of sediment-associated metals is largely determined by the dynamics of the fluvial transport system, we still lack a process-based understanding of the spatial and temporal mechanisms that affect the physical and geochemical transfer of metals through catchments. This interdisciplinary project will exploit advances in geomorphic and geochemical analyses to develop a methodological approach and conceptual framework to answer key questions related to the dynamics and timescales of metal cycling in fluvial systems. The approach will be tested in two reaches of the mining-impacted Afon Twymyn, Wales. The main objectives are: (i) quantify the physical transport of sediment and metals over a range of river flows and model sediment pathways; (ii) establish the geochemical mobility and speciation of sediment-associated metals and how this is modified through the sediment pathways. To achieve these objectives a geomorphic-geochemical combined methodology will be applied. It includes: (i) Aerial imagery that will be acquired from UAV surveys pre- and post-high flows and transformed into high-resolution DEMs using Structure-from-Motion; (ii) suspended sediment flux will be estimated indirectly by field calibration with a logging turbidimeter; (iii) 2D hydraulic and sediment transport model (Delft3D) will be used to quantify the transport of sediment and associated metals and to map the source, pathway and sink of contaminated sediment; (iv) soil and sediment samples (including suspended sediment) will be collected pre- and post-high flows for geochemical (concentration, speciation) and mineralogical (XRD, SEM) analyses; (v) finally, a geochemical model (Geochemists Workbench) will be developed to generate hypotheses that explain observed geochemical change as a function

  13. High frequency pressure oscillator for microcryocoolers.

    PubMed

    Vanapalli, S; ter Brake, H J M; Jansen, H V; Zhao, Y; Holland, H J; Burger, J F; Elwenspoek, M C

    2008-04-01

    Microminiature pulse tube cryocoolers should operate at a frequency of an order higher than the conventional macro ones because the pulse tube cryocooler operating frequency scales inversely with the square of the pulse tube diameter. In this paper, the design and experiments of a high frequency pressure oscillator is presented with the aim to power a micropulse tube cryocooler operating between 300 and 80 K, delivering a cooling power of 10 mW. Piezoelectric actuators operate efficiently at high frequencies and have high power density making them good candidates as drivers for high frequency pressure oscillator. The pressure oscillator described in this work consists of a membrane driven by a piezoelectric actuator. A pressure ratio of about 1.11 was achieved with a filling pressure of 2.5 MPa and compression volume of about 22.6 mm(3) when operating the actuator with a peak-to-peak sinusoidal voltage of 100 V at a frequency of 1 kHz. The electrical power input was 2.73 W. The high pressure ratio and low electrical input power at high frequencies would herald development of microminiature cryocoolers. PMID:18447548

  14. Transponder System for High-Frequency Ranging

    NASA Technical Reports Server (NTRS)

    Lichtenberg, C. L.; Shores, P. W.; Kobayashi, H. S.

    1986-01-01

    Transponder system uses phase difference between transmitted and reflected high-frequency radio waves to measure distance to target. To suppress spurious measurements of reflections from objects near target at transmitted frequency and its harmonics, transponder at target generates return signal at half transmitted frequency. System useful in such applications as surveying, docking of ships, and short-range navigation.

  15. Psychophysical tuning curves at very high frequencies

    NASA Astrophysics Data System (ADS)

    Yasin, Ifat; Plack, Christopher J.

    2005-10-01

    For most normal-hearing listeners, absolute thresholds increase rapidly above about 16 kHz. One hypothesis is that the high-frequency limit of the hearing-threshold curve is imposed by the transmission characteristics of the middle ear, which attenuates the sound input [Masterton et al., J. Acoust. Soc. Am. 45, 966-985 (1969)]. An alternative hypothesis is that the high-frequency limit of hearing is imposed by the tonotopicity of the cochlea [Ruggero and Temchin, Proc. Nat. Acad. Sci. U.S.A. 99, 13206-13210 (2002)]. The aim of this study was to test these hypotheses. Forward-masked psychophysical tuning curves (PTCs) were derived for signal frequencies of 12-17.5 kHz. For the highest signal frequencies, the high-frequency slopes of some PTCs were steeper than the slope of the hearing-threshold curve. The results also show that the human auditory system displays frequency selectivity for characteristic frequencies (CFs) as high as 17 kHz, above the frequency at which absolute thresholds begin to increase rapidly. The findings suggest that, for CFs up to 17 kHz, the high-frequency limitation in humans is imposed in part by the middle-ear attenuation, and not by the tonotopicity of the cochlea.

  16. Linking Sediment Microbial Communities to Carbon Cycling in High-Latitude Lakes

    NASA Astrophysics Data System (ADS)

    Emerson, J. B.; Varner, R. K.; Johnson, J. E.; Owusu-Dommey, A.; Binder, M.; Woodcroft, B. J.; Wik, M.; Freitas, N. L.; Boyd, J. A.; Crill, P. M.; Saleska, S. R.; Tyson, G. W.; Rich, V. I.

    2015-12-01

    It is well recognized that thawing permafrost peatlands are likely to provide a positive feedback to climate change via CH4 and CO2 emissions. High-latitude lakes in these landscapes have also been identified as sources of CH4 and CO2 loss to the atmosphere. To investigate microbial contributions to carbon loss from high-latitude lakes, we characterized sediment geochemistry and microbiota via cores collected from deep and shallow regions of two lakes (Inre Harrsjön and Mellersta Harrsjön) in Arctic Sweden in July, 2012. These lakes are within the Stordalen Mire long-term ecological area, a focal site for investigating the impacts of climate change-related permafrost thaw, and the lakes in this area are responsible for ~55% of the CH4 loss from this hydrologically interconnected system. Across 40 samples from 4 to 40 cm deep within four sediment cores, Illumina 16S rRNA gene sequencing revealed that the sedimentary microbiota was dominated by candidate phyla OP9 and OP8 (Atribacteria and Aminicenantes, respectively, including putative fermenters and anaerobic respirers), predicted methanotrophic Gammaproteobacteria, and predicted methanogenic archaea from the Thermoplasmata Group E2 clade. We observed some overlap in community structure with nearby peatlands, which tend to be dominated by methanogens and Acidobacteria. Sediment microbial communities differed significantly between lakes, by overlying lake depth (shallow vs. deep), and by depth within a core, with each trend corresponding to parallel differences in biogeochemical measurements. Overall, our results support the potential for significant microbial controls on carbon cycling in high-latitude lakes associated with thawing permafrost, and ongoing metagenomic analyses of focal samples will yield further insight into the functional potential of these microbial communities and their dominant members.

  17. Impacts of shrimp farming cultivation cycles on macrobenthic assemblages and chemistry of sediments.

    PubMed

    Ribeiro, Luisa F; Eça, Gilmara F; Barros, Francisco; Hatje, Vanessa

    2016-04-01

    The aim of this study was to evaluate the influence of a shrimp farm cultivation cycle in the composition of sediments and on the structure of macrobenthic assemblages. Concentrations of nutrients, Zn and Cu were significantly higher in impact than control areas. In general, the level of contaminants was highest during the harvesting period and in sites closest to the discharge of effluents. Abundances and number of taxa of benthic invertebrates were at least one order of magnitude smaller in impacted areas than in controls. The structure of the benthic assemblages was significantly different at these two treatments. The combined use of biological and chemical data showed to be efficient to provide precise answers regarding the extent of impacts caused by shrimp cultivation. The results provide the basis for a better understanding of impacts of this activity and can subsidize the development of better management practices for coastal areas. PMID:26774777

  18. Marine Snow and Gels: Hot Spots of Biogeochemical Cycling, Biological Activity, and Sedimentation in the Sea

    NASA Astrophysics Data System (ADS)

    Alldredge, A. L.

    2004-12-01

    Much of the organic carbon sequestered in the deep sea and ocean bottom sediments as relatively rare, large detrital particles generically known as marine snow. Because they are enriched in organic matter, microbes, and nutrients, these large particles also serve as hot spots for biological and chemical process in the water column. Recent evidence reveals that abundant carbohydrate gel particles in the ocean, formed from the dissolved exudates of phytoplankton and bacteria, are intricately involved in the formation of marine snow. These discoveries are changing the way we conceptualize the pelagic zone on small scales. We no longer imagine seawater as a relatively homogeneous fluid in which float a spectrum of dispersed molecules, particles, and organisms, but instead see it as a rich hydrated matrix of transparent organic gels, detritus, and cob-web like surfaces which provide microscale physical, chemical, and biological structure. This talk will focus on the origins, fate, and significance of marine snow and gels in the sea, including their role in carbon cycling, sedimentation and carbon flux , food webs, and chemical and biological transformation.

  19. The link between marine sediment records and changes in Holocene Saharan landscape: simulating the dust cycle

    NASA Astrophysics Data System (ADS)

    Egerer, Sabine; Claussen, Martin; Reick, Christian; Stanelle, Tanja

    2016-04-01

    Marine sediment records reveal an abrupt and strong increase in dust deposition in the North Atlantic at the end of the African Humid Period about 4.9 to 5.5 ka ago. The change in dust flux has been attributed to varying Saharan land surface cover. Alternatively, the enhanced dust accumulation is linked to enhanced surface winds and a consequent intensification of coastal upwelling. Here we demonstrate for the first time the direct link between dust accumulation in marine cores and changes in Saharan land surface. We simulate the mid-Holocene (6 ka BP) and pre-industrial (1850 AD) dust cycle as a function of Saharan land surface cover and atmosphere-ocean conditions using the coupled atmosphere-aerosol model ECHAM6.1-HAM2.1. Mid-Holocene surface characteristics, including vegetation cover and lake surface area, are derived from proxy data and simulations. In agreement with data from marine sediment cores, our simulations show that mid-Holocene dust deposition fluxes in the North Atlantic were two to three times lower compared with pre-industrial fluxes. We identify Saharan land surface characteristics to be the main control on dust transport from North Africa to the North Atlantic. We conclude that the increase in dust accumulation in marine cores is directly linked to a transition of the Saharan landscape during the Holocene and not due to changes in atmospheric or ocean conditions alone.

  20. Intense nitrogen cycling in permeable intertidal sediment revealed by a nitrous oxide hot spot

    NASA Astrophysics Data System (ADS)

    Schutte, Charles A.; Joye, Samantha B.; Wilson, Alicia M.; Evans, Tyler; Moore, Willard S.; Casciotti, Karen

    2015-10-01

    Approximately 40% of the total global rate of nitrogen fixation is the result of human activities, and most of this anthropogenic nitrogen is used to fertilize agricultural fields. Approximately 23% of the applied agricultural nitrogen is delivered to the coastal zone, often reducing water quality and driving eutrophication. Nitrogen cycling in coastal sediments can mitigate eutrophication by removing bioavailable nitrogen. However, some of these processes generate nitrous oxide, a potent greenhouse gas, as a by-product. Here we report the discovery of a nitrous oxide production hot spot in shallow barrier island sands. Nitrous oxide concentrations, production and consumption rates, vertical diffusion fluxes, and flux to the atmosphere were measured across triplicate depth profiles. Using a mass balance approach, rates of net nitrous oxide production were estimated to be 40 µmol m-2 d-1. This production was driven by a hot spot of nitrate consumption that removed bioavailable nitrogen from the coastal environment at a rate of 10 mmol m-2 d-1, a rate that is comparable with the highest rates of denitrification reported for coastal sediments.

  1. Possible roles of uncultured archaea in carbon cycling in methane-seep sediments

    NASA Astrophysics Data System (ADS)

    Yoshinaga, Marcos Y.; Lazar, Cassandre S.; Elvert, Marcus; Lin, Yu-Shih; Zhu, Chun; Heuer, Verena B.; Teske, Andreas; Hinrichs, Kai-Uwe

    2015-09-01

    Studies on microbial carbon cycling uniformly confirm that anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria represent the dominant and most active fraction of the sedimentary microbial community in methane-seep sediments. However, little is known about other frequently observed and abundant microbial taxa, their role in carbon cycling and association with the anaerobic oxidation of methane (AOM). Here, we provide a comprehensive characterization of stable carbon isotopes (δ13C) from several intact polar lipid (IPL) classes and metabolite pools in a downcore profile at a cold seep within the oxygen minimum zone off Pakistan. We aimed to evaluate microbial carbon metabolism using IPLs in relation to redox conditions, metabolites and 16S rRNA gene libraries. The 13C-depleted signature of carbon pools and microbial metabolites in pore waters (e.g., dissolved inorganic carbon, lactate and acetate) demonstrated high accumulation of AOM-associated biomass and subsequent turnover thereof. ANMEs accounted for a small fraction of the archaeal 16S rRNA gene survey, whereas sequences of other uncultured benthic archaea dominated the clone libraries, particularly the Marine Benthic Group D. On the basis of lipid diversity and carbon isotope information, we suggest that structurally diverse phospho- and glycolipids, including the recently identified unsaturated tetraethers that are particularly abundant in this setting, are likely derived from archaea other than ANMEs. Through the evaluation of δ13C values of individual IPL, our results indicate heterotrophy as a possible metabolic pathway of archaea in these AOM-dominated sediments.

  2. Source-to-sink cycling of aeolian sediment in the north polar region of Mars

    NASA Astrophysics Data System (ADS)

    Ewing, R. C.; Kocurek, G.

    2012-12-01

    Aeolian sand dunes are prominent features on the landscapes of Earth, Mars, Venus and Titan and sedimentary deposits interpreted as aeolian in origin are found in the rock records of Earth and Mars. The widespread occurrence of aeolian dunes on the surface of these worlds and within their deep-time depositional records suggests that aeolian systems are and likely have been a default depositional environment for the Solar System. Within an aeolian source-to-sink context, we hypothesize that planet-specific boundary conditions strongly impact production, transport, accumulation and preservation of aeolian sediment, whereas dunes and dune-field patterns remain largely similar. This hypothesis is explored within the north polar region of Mars, which hosts the most extensive aeolian dune fields and aeolian sedimentary deposits yet recognized on Mars and appears to be a region of dynamic source-to-sink cycling of aeolian sediments. The Planum Boreum Cavi Unit rests beneath north polar ice cap of Mars and is composed of several hundred meters of niveo-aeolian dune cross-stratification. The overall architecture of the unit consists of sets of preserved dune topography with an upward increase in the abundance of ice. Dune sets are defined by stabilized, polygonally fractured bounding surfaces, erosional bounding surfaces and typical internal lee foresets made of sediment and ice. The accumulation of the Cavi Unit is interpreted as occurring through freezing and serves as an example of a cold temperature boundary condition on aeolian sediment accumulation. Preservation of the Cavi Unit arises because of deposition of the overlying ice cap and contrasts with preservation of aeolian sediment on Earth, which is largely driven by eustasy and tectonics. The Cavi Unit is thought to be one source of sediment for the north polar Olympia Undae Dune Field. The region of Olympia Undae near the Cavi Unit shows a reticulate dune field pattern composed of two sets of nearly orthogonal

  3. Microbial Fe cycling and mineralization in sediments of an acidic, hypersaline lake (Lake Tyrell, Victoria, Australia)

    NASA Astrophysics Data System (ADS)

    Roden, E. E.; Blöthe, M.; Shelobolina, E.

    2009-12-01

    Lake Tyrrell is a variably acidic, hypersaline, Fe-rich lake located in Victoria, Australia. Terrestrial acid saline lakes like Lake Tyrrell may be analogs for ancient Martian surface environments, as well as possible extant subsurface environments. To investigate the potential for microbial Fe cycling under acidic conditions and high salt concentration, we collected sediment core samples during three field trips between 2006 and 2008 from the southern, acidic edge of the lake. Materials from the cores were used for chemical and mineralogical analyses, as well as for molecular (16S rRNA genes) and culture-based microbiological studies. Near-surface (< 1 m depth) pore fluids contained low but detectable dissolved oxygen (ca. 50 uM), significant dissolved Fe(II) (ca. 500 uM), and nearly constant pH of around 4 - conditions conducive to enzymatic Fe(II) oxidation. High concentrations of Fe(III) oxides begin accumulate at a depth of ca. 10 cm, and may reflect the starting point for formation of massive iron concretions that are evident at and beneath the sediment surface. MPN analyses revealed low (10-100 cells/mL) but detectable populations of aerobic, halophilic Fe(II)-oxidizing organisms on the sediment surface and in the near-surface ground water. With culture-dependent methods at least three different halotolerant lithoautotrophic cultures growing on Fe(II), thiosulfate, or tetrathionate from different acidic sites were obtained. Analysis of 16S rRNA gene sequences revealed that these organisms are similar to previous described gamma proteobacteria Thiobacillus prosperus (95%), Halothiobacillus kellyi (99%), Salinisphaera shabanense (95%) and a Marinobacter species. (98%). 16S rRNA gene pyrosequencing data from two different sites with a pH range between 3 and 4.5 revealed a dominance of gamma proteobacteria. 16S rRNA gene pyrosequencing libraries from both cores were dominated by sequences related to the Ectothiorhodospiraceae family, which includes the taxa

  4. Biostimulation induces syntrophic interactions that impact C, S and N cycling in a sediment microbial community

    PubMed Central

    Handley, Kim M; VerBerkmoes, Nathan C; Steefel, Carl I; Williams, Kenneth H; Sharon, Itai; Miller, Christopher S; Frischkorn, Kyle R; Chourey, Karuna; Thomas, Brian C; Shah, Manesh B; Long, Philip E; Hettich, Robert L; Banfield, Jillian F

    2013-01-01

    Stimulation of subsurface microorganisms to induce reductive immobilization of metals is a promising approach for bioremediation, yet the overall microbial community response is typically poorly understood. Here we used proteogenomics to test the hypothesis that excess input of acetate activates complex community functioning and syntrophic interactions among autotrophs and heterotrophs. A flow-through sediment column was incubated in a groundwater well of an acetate-amended aquifer and recovered during microbial sulfate reduction. De novo reconstruction of community sequences yielded near-complete genomes of Desulfobacter (Deltaproteobacteria), Sulfurovum- and Sulfurimonas-like Epsilonproteobacteria and Bacteroidetes. Partial genomes were obtained for Clostridiales (Firmicutes) and Desulfuromonadales-like Deltaproteobacteria. The majority of proteins identified by mass spectrometry corresponded to Desulfobacter-like species, and demonstrate the role of this organism in sulfate reduction (Dsr and APS), nitrogen fixation and acetate oxidation to CO2 during amendment. Results indicate less abundant Desulfuromonadales, and possibly Bacteroidetes, also actively contributed to CO2 production via the tricarboxylic acid (TCA) cycle. Proteomic data indicate that sulfide was partially re-oxidized by Epsilonproteobacteria through nitrate-dependent sulfide oxidation (using Nap, Nir, Nos, SQR and Sox), with CO2 fixed using the reverse TCA cycle. We infer that high acetate concentrations, aimed at stimulating anaerobic heterotrophy, led to the co-enrichment of, and carbon fixation in Epsilonproteobacteria. Results give an insight into ecosystem behavior following addition of simple organic carbon to the subsurface, and demonstrate a range of biological processes and community interactions were stimulated. PMID:23190730

  5. Determining the Effects of Oiled Sediment on Fish Life Cycle Endpoints using the Sheepshead Minnow (Cyprinodon variegatus)

    EPA Science Inventory

    Determination of long-term effects of exposure to crude oil is critical for ascertaining population-level risk following spill events. A 19-week life-cycle experiment was conducted with the estuarine sheepshead minnow exposed to natural sediment spiked with weathered Louisiana S...

  6. High-current, high-frequency capacitors

    NASA Technical Reports Server (NTRS)

    Renz, D. D.

    1983-01-01

    The NASA Lewis high-current, high-frequency capacitor development program was conducted under a contract with Maxwell Laboratories, Inc., San Diego, California. The program was started to develop power components for space power systems. One of the components lacking was a high-power, high-frequency capacitor. Some of the technology developed in this program may be directly usable in an all-electric airplane. The materials used in the capacitor included the following: the film is polypropylene, the impregnant is monoisopropyl biphenyl, the conductive epoxy is Emerson and Cuming Stycast 2850 KT, the foil is aluminum, the case is stainless steel (304), and the electrode is a modified copper-ceramic.

  7. Turbulence in unsteady flow at high frequencies

    NASA Technical Reports Server (NTRS)

    Kuhn, Gary D.

    1990-01-01

    Turbulent flows subjected to oscillations of the mean flow were simulated using a large-eddy simulation computer code for flow in a channel. The objective of the simulations was to provide better understanding of the effects of time-dependent disturbances on the turbulence of a boundary layer and of the underlying physical phenomena regarding the basic interaction between the turbulence and external disturbances. The results confirmed that turbulence is sensitive to certain ranges of frequencies of disturbances. However, no direct connection was found between the frequency of imposed disturbances and the characteristic 'burst' frequency of turbulence. New insight into the nature of turbulence at high frequencies was found. Viscous phenomena near solid walls were found to be the dominant influence for high-frequency perturbations.

  8. Apparatus for measuring high frequency currents

    NASA Technical Reports Server (NTRS)

    Hagmann, Mark J. (Inventor); Sutton, John F. (Inventor)

    2003-01-01

    An apparatus for measuring high frequency currents includes a non-ferrous core current probe that is coupled to a wide-band transimpedance amplifier. The current probe has a secondary winding with a winding resistance that is substantially smaller than the reactance of the winding. The sensitivity of the current probe is substantially flat over a wide band of frequencies. The apparatus is particularly useful for measuring exposure of humans to radio frequency currents.

  9. [High-frequency oscillatory ventilation in neonates].

    PubMed

    2002-09-01

    High-frequency oscillatory ventilation (HFOV) may be considered as an alternative in the management of severe neonatal respiratory failure requiring mechanical ventilation. In patients with diffuse pulmonary disease, HFOV can applied as a rescue therapy with a high lung volume strategy to obtain adequate alveolar recruitment. We review the mechanisms of gas exchange, as well as the indications, monitoring and special features of the use HVOF in the neonatal period. PMID:12199947

  10. Extremely high frequency RF effects on electronics.

    SciTech Connect

    Loubriel, Guillermo Manuel; Vigliano, David; Coleman, Phillip Dale; Williams, Jeffery Thomas; Wouters, Gregg A.; Bacon, Larry Donald; Mar, Alan

    2012-01-01

    The objective of this work was to understand the fundamental physics of extremely high frequency RF effects on electronics. To accomplish this objective, we produced models, conducted simulations, and performed measurements to identify the mechanisms of effects as frequency increases into the millimeter-wave regime. Our purpose was to answer the questions, 'What are the tradeoffs between coupling, transmission losses, and device responses as frequency increases?', and, 'How high in frequency do effects on electronic systems continue to occur?' Using full wave electromagnetics codes and a transmission-line/circuit code, we investigated how extremely high-frequency RF propagates on wires and printed circuit board traces. We investigated both field-to-wire coupling and direct illumination of printed circuit boards to determine the significant mechanisms for inducing currents at device terminals. We measured coupling to wires and attenuation along wires for comparison to the simulations, looking at plane-wave coupling as it launches modes onto single and multiconductor structures. We simulated the response of discrete and integrated circuit semiconductor devices to those high-frequency currents and voltages, using SGFramework, the open-source General-purpose Semiconductor Simulator (gss), and Sandia's Charon semiconductor device physics codes. This report documents our findings.

  11. Ionospheric modifications in high frequency heating experiments

    SciTech Connect

    Kuo, Spencer P.

    2015-01-15

    Featured observations in high-frequency (HF) heating experiments conducted at Arecibo, EISCAT, and high frequency active auroral research program are discussed. These phenomena appearing in the F region of the ionosphere include high-frequency heater enhanced plasma lines, airglow enhancement, energetic electron flux, artificial ionization layers, artificial spread-F, ionization enhancement, artificial cusp, wideband absorption, short-scale (meters) density irregularities, and stimulated electromagnetic emissions, which were observed when the O-mode HF heater waves with frequencies below foF2 were applied. The implication and associated physical mechanism of each observation are discussed and explained. It is shown that these phenomena caused by the HF heating are all ascribed directly or indirectly to the excitation of parametric instabilities which instigate anomalous heating. Formulation and analysis of parametric instabilities are presented. The results show that oscillating two stream instability and parametric decay instability can be excited by the O-mode HF heater waves, transmitted from all three heating facilities, in the regions near the HF reflection height and near the upper hybrid resonance layer. The excited Langmuir waves, upper hybrid waves, ion acoustic waves, lower hybrid waves, and field-aligned density irregularities set off subsequent wave-wave and wave-electron interactions, giving rise to the observed phenomena.

  12. Abrupt Climate Change During the Last Glacial Cycle Based on Gulf of Mexico Sediments

    NASA Astrophysics Data System (ADS)

    Flower, B. P.; Hastings, D. W.; Hill, H.; Quinn, T. M.

    2003-12-01

    Evidence is emerging that the tropical climate system played a major role in past global climate change during the last glacial cycle. However, existing studies indicate asynchronous temperature variability in the western equatorial Atlantic, complicating the identification of causal mechanisms. Because the Gulf of Mexico (GOM) is linked to the equatorial Atlantic, sea-surface temperature (SST) records from the GOM help assess the phasing between low- and high-latitude Atlantic climate. High sedimentation rates of >40 cm/k.y. and laminated sediments in Orca Basin allow sub-centennial-scale resolution. Paired δ 18O and Mg/Ca data on the planktic foraminifer Globigerinoides ruber from core EN32-PC6 are used to separate deglacial changes in SST and δ 18Oseawater due to low-salinity meltwater from the Laurentide Ice Sheet (LIS). Mg-SST increases by >3.0° C between 17.2 and 15.2 ka (calendar years) encompassing Heinrich Event 1 in the North Atlantic. Comparison to polar ice core records indicates GOM SST was not in phase with Greenland air temperature, consistent with thermohaline circulation modulation of Atlantic climate. This warming represents the bulk of the 4.2+/-0.9° C increase from the last glacial maximum (24.0+/-0.8° C) to early Holocene (29.0+/-0.4° C). Subtracting temperature and ice-volume effects from Gs. ruber δ 18O reveals two episodes of LIS meltwater input, one of >1.5% from ca. 16.2-15.7 ka and a second major spike of >2% from ca. 15.2-13.0 ka that encompassed meltwater pulse 1A (mwp-1A) and peaked at ca. 13.4 ka. These results suggest that (1) subtropical Atlantic SST warming preceded peak LIS decay and mwp-1A by >2 k.y., (2) thermohaline circulation may have modulated Atlantic climate on the millennial scale during the last deglaciation, and (3) major LIS meltwater input to the GOM ended before North Atlantic Deep Water suppression during the Younger Dryas. A new 31.79 m Calypso piston core collected in July 2002 on the R/V Marion Dufresne

  13. Fe-phyllosilicate redox cycling organisms from a redox transition zone in Hanford 300 Area sediments

    PubMed Central

    Benzine, Jason; Xiong, Mai Yia; Kennedy, David W.; McKinley, James P.; Lin, Xueju; Roden, Eric E.

    2013-01-01

    Microorganisms capable of reducing or oxidizing structural iron (Fe) in Fe-bearing phyllosilicate minerals were enriched and isolated from a subsurface redox transition zone at the Hanford 300 Area site in eastern Washington, USA. Both conventional and in situ “i-chip” enrichment strategies were employed. One Fe(III)-reducing Geobacter (G. bremensis strain R1, Deltaproteobacteria) and six Fe(II) phyllosilicate-oxidizing isolates from the Alphaproteobacteria (Bradyrhizobium japonicum strains 22, is5, and in8p8), Betaproteobacteria (Cupriavidus necator strain A5-1, Dechloromonas agitata strain is5), and Actinobacteria (Nocardioides sp. strain in31) were recovered. The G. bremensis isolate grew by oxidizing acetate with the oxidized form of NAu-2 smectite as the electron acceptor. The Fe(II)-oxidizers grew by oxidation of chemically reduced smectite as the energy source with nitrate as the electron acceptor. The Bradyrhizobium isolates could also carry out aerobic oxidation of biotite. This is the first report of the recovery of a Fe(II)-oxidizing Nocardioides, and to date only one other Fe(II)-oxidizing Bradyrhizobium is known. The 16S rRNA gene sequences of the isolates were similar to ones found in clone libraries from Hanford 300 sediments and groundwater, suggesting that such organisms may be present and active in situ. Whole genome sequencing of the isolates is underway, the results of which will enable comparative genomic analysis of mechanisms of extracellular phyllosilicate Fe redox metabolism, and facilitate development of techniques to detect the presence and expression of genes associated with microbial phyllosilicate Fe redox cycling in sediments. PMID:24379809

  14. Fe-phyllosilicate redox cycling organisms from a redox transition zone in Hanford 300 Area sediments

    SciTech Connect

    Benzine, Jason; Shelobolina, Evgenya S.; Xiong, Mai Yia; Kennedy, David W.; McKinley, James P.; Lin, Xueju; Roden, Eric E.

    2013-01-01

    Microorganisms capable of reducing or oxidizing structural iron (Fe) in Fe-bearing phyllosilicate minerals were enriched and isolated from a subsurface redox transition zone at the Hanford 300 Area site in eastern Washington, USA. Both conventional and in situ "i-chip" enrichment strategies were employed. One Fe(III)-reducing Geobacter (G. bremensis strain R1, Deltaproteobacteria) and six Fe(II) phyllosilicate-oxidizing isolates from the Alphaproteobacteria (Bradyrhizobium japonicum strains 22, is5, and in8p8), Betaproteobacteria (Cupriavidus necator strain A5-1, Dechloromonas agitata strain is5), and Actinobacteria (Nocardioides sp. strain in31) were recovered. The G. bremensis isolate grew by oxidizing acetate with the oxidized form of NAu-2 smectite as the electron acceptor. The Fe(II)-oxidizers grew by oxidation of chemically reduced smectite as the energy source with nitrate as the electron acceptor. The Bradyrhizobium isolates could also carry out aerobic oxidation of biotite. This is the first report of the recovery of a Fe(II)-oxidizing Nocardioides, and to date only one other Fe(II)-oxidizing Bradyrhizobium is known. The 16S rRNA gene sequences of the isolates were similar to ones found in clone libraries from Hanford 300 sediments and groundwater, suggesting that such organisms may be present and active in situ. Whole genome sequencing of the isolates is underway, the results of which will enable comparative genomic analysis of mechanisms of extracellular phyllosilicate Fe redox metabolism, and facilitate development of techniques to detect the presence and expression of genes associated with microbial phyllosilicate Fe redox cycling in sediments.

  15. Stable isotope biogeochemistry of the sulfur cycle in modern marine sediments: I. Seasonal dynamics in a temperate intertidal sandy surface sediment.

    PubMed

    Böttcher, Michael; Hespenheide, Britta; Brumsack, Hans-Jürgen; Bosselmann, Katja

    2004-12-01

    A biogeochemical and stable isotope geochemical study was carried out in surface sediments of an organic-matter poor temperate intertidal sandy surface sediment (German Wadden Sea of the North Sea) to investigate the activity of sulfate-reducing bacteria and the dynamics of the vertical partitioning of sedimentary sulfur, iron, and manganese species in relation to the availability of total organic carbon (TOC) and mud contents. The contents and stable isotopic compositions ((34)S/(32)S) of total reduced inorganic sulfur species (TRIS) and dissolved sulfate were measured. Maximum oxygen penetration depths were estimated from the onset of a blackening of the sediments due to FeS accumulation and ranged from 5 to 10 mm below surface (mmbsf). A zone of relatively moderate relative organic-matter enrichment was found between 5 and 20 mmbsf leading to enhanced activities of sulfate-reducing bacteria with sulfate-reduction rates (SRR) up to 350 nmol cm(-3) d(-1). Below this zone, microbial SRR dropped significantly. Depth integrated SRR seem to depend not only on temperature but also on the availability of reactive organic matter. The sulfur-isotopic composition of TRIS was depleted in (34)S by 33-40 per thousand with respect to coexisting dissolved sulfate (constant at about +21 per thousand vs. Vienna-Canyon Diablo Troilite (V-CDT)). Since sulfate reduction is not limited by dissolved sulfate (open system), depth variations of the isotopic composition of TRIS reflect changes in overall isotope effect due to superimposed microbial and abiotic reactions. Most of the solid-phase iron and manganese was bonded to (non-reactive) heavy minerals. However, a layer of reactive Fe(III) and Mn(IV) oxi(hydroxi)des was found in the uppermost sediment section due to re-oxidation of dissolved Fe(II) and Mn(II) species at the sediment-water interface. Metal cycling below the surface is at least partially coupled to intense sulfur cycling. PMID:15621745

  16. Role of freeze-thaw cycles and chlorpyrifos insecticide use on diffuse Cd loss and sediment accumulation

    NASA Astrophysics Data System (ADS)

    Wang, Fangli; Ouyang, Wei; Hao, Fanghua; Jiao, Wei; Shan, Yushu; Lin, Chunye

    2016-06-01

    Freeze-thaw cycles are predicted to increase in cold temperate regions. The potential influence of the interactions of freeze-thaw cycles and agrochemicals on the release of Cd into river water is unknown. In this study, the interactions of freeze-thaw cycles and chlorpyrifos (FC) on Cd mobility in soils were analysed. The spatial variability of soil Cd under long-term intensive tillage in a freeze-thaw agro-system was also identified. The temporal variation of sediment Cd was detected based on analysis of the sediment geochemistry. The results showed that FC increased soil Cd mobility, with an increase of approximately 10% in CaCl2-extractable Cd. The increased mobile fractions of water-soluble and exchangeable Cd originated from the decreased fraction of Fe-Mn-oxide-associated Cd and organic matter-bound Cd. The total Cd content in the surface soil followed the zonally decreasing trend of dry land > paddy land > natural land. The Cd concentrations and sedimentation rates of the sediment core generally increased from 1943 to 2013 due to agricultural exploration and farmland irrigation system construction, indicating an increase of the Cd input flux into water. The results provide valuable information about the soil Cd transport response to the influence of climatic and anthropogenic factors in cold intensive agro-systems.

  17. Role of freeze-thaw cycles and chlorpyrifos insecticide use on diffuse Cd loss and sediment accumulation

    PubMed Central

    Wang, Fangli; Ouyang, Wei; Hao, Fanghua; Jiao, Wei; Shan, Yushu; Lin, Chunye

    2016-01-01

    Freeze-thaw cycles are predicted to increase in cold temperate regions. The potential influence of the interactions of freeze-thaw cycles and agrochemicals on the release of Cd into river water is unknown. In this study, the interactions of freeze-thaw cycles and chlorpyrifos (FC) on Cd mobility in soils were analysed. The spatial variability of soil Cd under long-term intensive tillage in a freeze-thaw agro-system was also identified. The temporal variation of sediment Cd was detected based on analysis of the sediment geochemistry. The results showed that FC increased soil Cd mobility, with an increase of approximately 10% in CaCl2-extractable Cd. The increased mobile fractions of water-soluble and exchangeable Cd originated from the decreased fraction of Fe-Mn-oxide-associated Cd and organic matter-bound Cd. The total Cd content in the surface soil followed the zonally decreasing trend of dry land > paddy land > natural land. The Cd concentrations and sedimentation rates of the sediment core generally increased from 1943 to 2013 due to agricultural exploration and farmland irrigation system construction, indicating an increase of the Cd input flux into water. The results provide valuable information about the soil Cd transport response to the influence of climatic and anthropogenic factors in cold intensive agro-systems. PMID:27250820

  18. Role of freeze-thaw cycles and chlorpyrifos insecticide use on diffuse Cd loss and sediment accumulation.

    PubMed

    Wang, Fangli; Ouyang, Wei; Hao, Fanghua; Jiao, Wei; Shan, Yushu; Lin, Chunye

    2016-01-01

    Freeze-thaw cycles are predicted to increase in cold temperate regions. The potential influence of the interactions of freeze-thaw cycles and agrochemicals on the release of Cd into river water is unknown. In this study, the interactions of freeze-thaw cycles and chlorpyrifos (FC) on Cd mobility in soils were analysed. The spatial variability of soil Cd under long-term intensive tillage in a freeze-thaw agro-system was also identified. The temporal variation of sediment Cd was detected based on analysis of the sediment geochemistry. The results showed that FC increased soil Cd mobility, with an increase of approximately 10% in CaCl2-extractable Cd. The increased mobile fractions of water-soluble and exchangeable Cd originated from the decreased fraction of Fe-Mn-oxide-associated Cd and organic matter-bound Cd. The total Cd content in the surface soil followed the zonally decreasing trend of dry land > paddy land > natural land. The Cd concentrations and sedimentation rates of the sediment core generally increased from 1943 to 2013 due to agricultural exploration and farmland irrigation system construction, indicating an increase of the Cd input flux into water. The results provide valuable information about the soil Cd transport response to the influence of climatic and anthropogenic factors in cold intensive agro-systems. PMID:27250820

  19. High-frequency Rayleigh-wave method

    USGS Publications Warehouse

    Xia, J.; Miller, R.D.; Xu, Y.; Luo, Y.; Chen, C.; Liu, J.; Ivanov, J.; Zeng, C.

    2009-01-01

    High-frequency (???2 Hz) Rayleigh-wave data acquired with a multichannel recording system have been utilized to determine shear (S)-wave velocities in near-surface geophysics since the early 1980s. This overview article discusses the main research results of high-frequency surface-wave techniques achieved by research groups at the Kansas Geological Survey and China University of Geosciences in the last 15 years. The multichannel analysis of surface wave (MASW) method is a non-invasive acoustic approach to estimate near-surface S-wave velocity. The differences between MASW results and direct borehole measurements are approximately 15% or less and random. Studies show that simultaneous inversion with higher modes and the fundamental mode can increase model resolution and an investigation depth. The other important seismic property, quality factor (Q), can also be estimated with the MASW method by inverting attenuation coefficients of Rayleigh waves. An inverted model (S-wave velocity or Q) obtained using a damped least-squares method can be assessed by an optimal damping vector in a vicinity of the inverted model determined by an objective function, which is the trace of a weighted sum of model-resolution and model-covariance matrices. Current developments include modeling high-frequency Rayleigh-waves in near-surface media, which builds a foundation for shallow seismic or Rayleigh-wave inversion in the time-offset domain; imaging dispersive energy with high resolution in the frequency-velocity domain and possibly with data in an arbitrary acquisition geometry, which opens a door for 3D surface-wave techniques; and successfully separating surface-wave modes, which provides a valuable tool to perform S-wave velocity profiling with high-horizontal resolution. ?? China University of Geosciences (Wuhan) and Springer-Verlag GmbH 2009.

  20. Biostimulation induces syntrophic interactions that impact C, S and N cycling in a sediment microbial community

    SciTech Connect

    Handley, KM; Verberkmoes, Nathan C; Steefel, Carl I; Sharon, I; Williams, Ken; Miller, CS; Frischkorn, Kyle C; Chourey, Karuna; Thomas, Brian; Shah, Manesh B; Long, Phil; Hettich, Robert {Bob} L; Banfield, Jillian F.

    2013-01-01

    Stimulation of subsurface microorganisms to induce reductive immobilization of metals is a promising approach for bioremediation, yet the overall microbial community response is typically poorly understood. Here we used community proteogenomics to test the hypothesis that excess input of acetate activates syntrophic interactions among autotrophs and heterotrophs. A flow-through sediment column was incubated in a groundwater well of an acetate-amended aquifer. Genomic sequences from the community recovered during microbial sulfate reduction were used to econstruct, de novo, near-complete genomes for Desulfobacter (Deltaproteobacteria) and relatives of Sulfurovum and Sulfurimonas (Epsilonproteobacteria), and Bacteroidetes. Partial genomes were obtained for Clostridiales (Firmicutes) and Desulfuromonadales-like Deltaproteobacteria. The majority of proteins identified by mass spectrometry corresponded to Desulfobacter-like species, and demonstrate the role of this organism in sulfate reduction (Dsr and APS), nitrogen-fixation (Nif) and acetate oxidation to CO2 during amendment. Results suggest less abundant Desulfuromonadales and Bacteroidetes also actively contributed to CO2 production via the TCA cycle. Proteomic data indicate that sulfide was partially re-oxidized by Epsilonproteobacteria through nitrate-dependent sulfide oxidation (using Nap, Nir, Nos, SQR and Sox), with CO2 fixed using the reverse TCA cycle. Modeling shows that this reaction was thermodynamically possible, and kinetically favorable relative to acetate-dependent denitrification. We conclude that high-levels of carbon amendment aimed to stimulate anaerobic heterotrophy led to carbon fixation in co-dependent chemoautotrophs. These results have implications for understanding complex ecosystem behavior, and show that high levels of organic carbon supplementation can expand the range of microbial functionalities accessible for ecosystem manipulation.

  1. High frequency ultrasonic mitigation of microbial corrosion

    NASA Astrophysics Data System (ADS)

    Almahamedh, Hussain H.; Meegan, G. Douglas; Mishra, Brajendra; Olson, David L.; Spear, John R.

    2012-05-01

    Microbiologically Influenced Corrosion (MIC) is a major problem in oil industry facilities, and considerable effort has been spent to mitigate this costly issue. More environmentally benign methods are under consideration as alternatives to biocides, among which are ultrasonic techniques. In this study, a high frequency ultrasonic technique (HFUT) was used as a mitigation method for MIC. The killing percentages of the HFUT were higher than 99.8 percent and their corrosivity on steel was reduced by more than 50 percent. The practice and result will be discussed.

  2. The LASI high-frequency ellipticity system

    SciTech Connect

    Sternberg, B.K.; Poulton, M.M.

    1995-12-31

    A high-frequency, high-resolution, electromagnetic (EM) imaging system has been developed for environmental geophysics surveys. Some key features of this system include: (1) rapid surveying to allow dense spatial sampling over a large area, (2) high-accuracy measurements which are used to produce a high-resolution image of the subsurface, (3) measurements which have excellent signal-to-noise ratio over a wide bandwidth (31 kHz to 32 MHz), (4) large-scale physical modeling to produce accurate theoretical responses over targets of interest in environmental geophysics surveys, (5) rapid neural network interpretation at the field site, and (6) visualization of complex structures during the survey.

  3. Inviscid fluid in high frequency excitation field

    NASA Technical Reports Server (NTRS)

    Zak, M.

    1984-01-01

    The influence of high frequency excitations (HFE) on a fluid is investigated. The response to these excitations is decomposed in two parts: 'slow' motion, which practically remains unchanged during the vanishingly small period tau, and 'fast' motion whose value during this period is negligible in terms of displacements, but is essential in terms of the kinetic energy. After such a decomposition the 'slow' and 'fast' motions become nonlinearly coupled by the corresponding governing equations. This coupling leads to an 'effective' potential energy which imparts some 'elastic' properties to the fluid and stabilizes laminar flows.

  4. The LASI high-frequency ellipticity system

    SciTech Connect

    Sternberg, B.K.; Poulton, M.M.

    1995-10-01

    A high-frequency, high-resolution, electromagnetic (EM) imaging system has been developed for environmental geophysics surveys. Some key features of this system include: (1) rapid surveying to allow dense spatial sampling over a large area, (2) high-accuracy measurements which are used to produce a high-resolution image of the subsurface, (3) measurements which have excellent signal-to-noise ratio over a wide bandwidth (31 kHz to 32 MHz), (4) large-scale physical modeling to produce accurate theoretical responses over targets of interest in environmental geophysics surveys, (5) rapid neural network interpretation at the field site, and (6) visualization of complex structures during the survey.

  5. Inverter design for high frequency power distribution

    NASA Technical Reports Server (NTRS)

    King, R. J.

    1985-01-01

    A class of simple resonantly commutated inverters are investigated for use in a high power (100 KW - 1000 KW) high frequency (10 KHz - 20 KHz) AC power distribution system. The Mapham inverter is found to provide a unique combination of large thyristor turn-off angle and good utilization factor, much better than an alternate 'current-fed' inverter. The effects of loading the Mapham inverter entirely with rectifier loads are investigated by simulation and with an experimental 3 KW 20 KHz inverter. This inverter is found to be well suited to a power system with heavy rectifier loading.

  6. LOSCAR: Long-term Ocean-atmosphere-Sediment CArbon cycle Reservoir Model v2.0.4

    NASA Astrophysics Data System (ADS)

    Zeebe, R. E.

    2012-01-01

    The LOSCAR model is designed to efficiently compute the partitioning of carbon between ocean, atmosphere, and sediments on time scales ranging from centuries to millions of years. While a variety of computationally inexpensive carbon cycle models are already available, many are missing a critical sediment component, which is indispensable for long-term integrations. One of LOSCAR's strengths is the coupling of ocean-atmosphere routines to a computationally efficient sediment module. This allows, for instance, adequate computation of CaCO3 dissolution, calcite compensation, and long-term carbon cycle fluxes, including weathering of carbonate and silicate rocks. The ocean component includes various biogeochemical tracers such as total carbon, alkalinity, phosphate, oxygen, and stable carbon isotopes. LOSCAR's configuration of ocean geometry is flexible and allows for easy switching between modern and paleo-versions. We have previously published applications of the model tackling future projections of ocean chemistry and weathering, pCO2 sensitivity to carbon cycle perturbations throughout the Cenozoic, and carbon/calcium cycling during the Paleocene-Eocene Thermal Maximum. The focus of the present contribution is the detailed description of the model including numerical architecture, processes and parameterizations, tuning, and examples of input and output. Typical CPU integration times of LOSCAR are of order seconds for several thousand model years on current standard desktop machines. The LOSCAR source code in C can be obtained from the author by sending a request to loscar.model@gmail.com.

  7. Molecular biological and isotopic biogeochemical prognoses of the nitrification-driven dynamic microbial nitrogen cycle in hadopelagic sediments.

    PubMed

    Nunoura, Takuro; Nishizawa, Manabu; Kikuchi, Tohru; Tsubouchi, Taishi; Hirai, Miho; Koide, Osamu; Miyazaki, Junichi; Hirayama, Hisako; Koba, Keisuke; Takai, Ken

    2013-11-01

    There has been much progress in understanding the nitrogen cycle in oceanic waters including the recent identification of ammonia-oxidizing archaea and anaerobic ammonia oxidizing (anammox) bacteria, and in the comprehensive estimation in abundance and activity of these microbial populations. However, compared with the nitrogen cycle in oceanic waters, there are fewer studies concerning the oceanic benthic nitrogen cycle. To further elucidate the dynamic nitrogen cycle in deep-sea sediments, a sediment core obtained from the Ogasawara Trench at a water depth of 9760 m was analysed in this study. The profiles obtained for the pore-water chemistry, and nitrogen and oxygen stable isotopic compositions of pore-water nitrate in the hadopelagic sediments could not be explained by the depth segregation of nitrifiers and nitrate reducers, suggesting the co-occurrence of nitrification and nitrate reduction in the shallowest nitrate reduction zone. The abundance of SSU rRNA and functional genes related to nitrification and denitrification are consistent with the co-occurrence of nitrification and nitrate reduction observed in the geochemical analyses. This study presents the first example of cooperation between aerobic and anaerobic nitrogen metabolism in the deep-sea sedimentary environments. PMID:23718903

  8. High Frequency Plasma Generators for Ion Thrusters

    NASA Technical Reports Server (NTRS)

    Divergilio, W. F.; Goede, H.; Fosnight, V. V.

    1981-01-01

    The results of a one year program to experimentally adapt two new types of high frequency plasma generators to Argon ion thrusters and to analytically study a third high frequency source concept are presented. Conventional 30 cm two grid ion extraction was utilized or proposed for all three sources. The two plasma generating methods selected for experimental study were a radio frequency induction (RFI) source, operating at about 1 MHz, and an electron cyclotron heated (ECH) plasma source operating at about 5 GHz. Both sources utilize multi-linecusp permanent magnet configurations for plasma confinement. The plasma characteristics, plasma loading of the rf antenna, and the rf frequency dependence of source efficiency and antenna circuit efficiency are described for the RFI Multi-cusp source. In a series of tests of this source at Lewis Research Center, minimum discharge losses of 220+/-10 eV/ion were obtained with propellant utilization of .45 at a beam current of 3 amperes. Possible improvement modifications are discussed.

  9. High frequency inductive lamp and power oscillator

    DOEpatents

    MacLennan, Donald A.; Turner, Brian P.; Dolan, James T.; Kirkpatrick, Douglas A.; Leng, Yongzhang

    2000-01-01

    A high frequency inductively coupled electrodeless lamp includes an excitation coil with an effective electrical length which is less than one half wavelength of a driving frequency applied thereto, preferably much less. The driving frequency may be greater than 100 MHz and is preferably as high as 915 MHz. Preferably, the excitation coil is configured as a non-helical, semi-cylindrical conductive surface having less than one turn, in the general shape of a wedding ring. At high frequencies, the current in the coil forms two loops which are spaced apart and parallel to each other. Configured appropriately, the coil approximates a Helmholtz configuration. The lamp preferably utilizes an bulb encased in a reflective ceramic cup with a pre-formed aperture defined therethrough. The ceramic cup may include structural features to aid in alignment and/or a flanged face to aid in thermal management. The lamp head is preferably an integrated lamp head comprising a metal matrix composite surrounding an insulating ceramic with the excitation integrally formed on the ceramic. A novel solid-state oscillator preferably provides RF power to the lamp. The oscillator is a single active element device capable of providing over 70 watts of power at over 70% efficiency. Various control circuits may be employed to match the driving frequency of the oscillator to a plurality of tuning states of the lamp.

  10. High frequency inductive lamp and power oscillator

    DOEpatents

    MacLennan, Donald A.; Dymond, Jr., Lauren E.; Gitsevich, Aleksandr; Grimm, William G.; Kipling, Kent; Kirkpatrick, Douglas A.; Ola, Samuel A.; Simpson, James E.; Trimble, William C.; Tsai, Peter; Turner, Brian P.

    2001-01-01

    A high frequency inductively coupled electrodeless lamp includes an excitation coil with an effective electrical length which is less than one half wavelength of a driving frequency applied thereto, preferably much less. The driving frequency may be greater than 100 MHz and is preferably as high as 915 MHz. Preferably, the excitation coil is configured as a non-helical, semi-cylindrical conductive surface having less than one turn, in the general shape of a wedding ring. At high frequencies, the current in the coil forms two loops which are spaced apart and parallel to each other. Configured appropriately, the coil approximates a Helmholtz configuration. The lamp preferably utilizes an bulb encased in a reflective ceramic cup with a pre-formed aperture defined therethrough. The ceramic cup may include structural features to aid in alignment and I or a flanged face to aid in thermal management. The lamp head is preferably an integrated lamp head comprising a metal matrix composite surrounding an insulating ceramic with the excitation integrally formed on the ceramic. A novel solid-state oscillator preferably provides RF power to the lamp. The oscillator is a single active element device capable of providing over 70 watts of power at over 70% efficiency. Various control circuits may be employed to adjust the driving frequency of the oscillator.

  11. Noise temperature in graphene at high frequencies

    NASA Astrophysics Data System (ADS)

    Rengel, Raúl; Iglesias, José M.; Pascual, Elena; Martín, María J.

    2016-07-01

    A numerical method for obtaining the frequency-dependent noise temperature in monolayer graphene is presented. From the mobility and diffusion coefficient values provided by Monte Carlo simulation, the noise temperature in graphene is studied up to the THz range, considering also the influence of different substrate types. The influence of the applied electric field is investigated: the noise temperature is found to increase with the applied field, dropping down at high frequencies (in the sub-THz range). The results show that the low-frequency value of the noise temperature in graphene on a substrate tends to be reduced as compared to the case of suspended graphene due to the important effect of remote polar phonon interactions, thus indicating a reduced emitted noise power; however, at very high frequencies the influence of the substrate tends to be significantly reduced, and the differences between the suspended and on-substrate cases tend to be minimized. The values obtained are comparable to those observed in GaAs and semiconductor nitrides.

  12. Growth responses of an estuarine fish exposed to mixed trace elements in sediments over a full life cycle.

    PubMed

    Rowe, Christopher L

    2003-02-01

    Hatchling Cyprinodon variegatus were raised in the presence or absence of sediments contaminated with mixed trace elements to examine lethal and sublethal bioenergetic effects (metabolic rate, lipid storage, growth, reproduction) over a full life cycle (>1 year). Contaminated sediments were derived from a site receiving coal combustion residues (CCR) and were elevated in numerous trace elements including Al, As, Ba, Cd, Cu, Se, and V. Exposures were conducted at two levels of salinity (5 and 36 ppt) to examine the potential interaction of this variable with contaminants. Salinity had no effect on responses measured. Over the course of the study, fish exposed to contaminated sediment accumulated several CCR-related trace elements, including As, Cd, Se, and V. There were no differences in fish survival for contaminated sediment treatments and uncontaminated sediment treatments, nor were there differences in metabolic expenditures. However, growth, male condition factor, and storage lipid content in females were reduced due to contaminant exposure. No significant effects on fecundity or the proportion of females that were gravid at the end of the study were observed, yet females raised under control conditions produced 12% larger eggs than did females raised on contaminated sediments. During the presumably most-sensitive early life stages, individuals were not noticeably affected by contaminants, but rather the effects of exposure became apparent later in life. Because many species inhabit contaminated areas for long periods of time, often encompassing the entire life cycle, exposures focused only on specific life stages may substantially underestimate the overall responses elicited by individuals. PMID:12550102

  13. Distribution and preservation of black carbon in the East China Sea sediments: Perspectives on carbon cycling at continental margins

    NASA Astrophysics Data System (ADS)

    Huang, Liang; Zhang, Jing; Wu, Ying; Wang, Jinlong

    2016-02-01

    We determined the concentrations and radiocarbon (14C) compositions of black carbon (BC) in the sediments of the East China Sea (ECS). The BC concentrations, which were in the range of 0.30-1.52 mg/g, accounted for 12-65% of the total organic carbon (TOC). The distribution of BC in ECS sediments was controlled by factors such as grain size, distance from the coast, and deposition rate. Radiocarbon measurements of BC yielded ages of 6350-10,440 years before present (BP), suggesting that the percentage of BC derived from biomass combustion was in the range of 29-48%. The BC burial flux in sediments of the ECS was estimated to be ∼1.39×106 t/yr, which was similar to burial fluxes reported for shelf sediments in other areas. However, the magnitude of the total BC sink was far greater than that of any other shelf regions studied to date, indicating the global importance of BC accumulation in the ECS, and the magnitude of BC input from large rivers (e.g., the Changjiang). The riverine delivery of BC to the ECS (73%) was far greater than that of atmospheric flux (27%). Further study of the BC cycle and the interactions of BC with other organic compounds in marginal seas was required to better understand the role of BC in the global carbon cycle.

  14. Plasma effects in high frequency radiative transfer

    SciTech Connect

    Alonso, C.T.

    1981-02-08

    This paper is intended as a survey of collective plasma processes which can affect the transfer of high frequency radiation in a hot dense plasma. We are rapidly approaching an era when this subject will become important in the laboratory. For pedagogical reasons we have chosen to examine plasma processes by relating them to a particular reference plasma which will consist of fully ionized carbon at a temperature kT=1 KeV (10/sup 70/K) and an electron density N = 3 x 10/sup 23/cm/sup -3/, (which corresponds to a mass density rho = 1 gm/cm/sup 3/ and an ion density N/sub i/ = 5 x 10/sup 22/ cm/sup -3/). We will consider the transport in such a plasma of photons ranging from 1 eV to 1 KeV in energy. Such photons will probably be frequently used as diagnostic probes of hot dense laboratory plasmas.

  15. Inline high frequency ultrasonic particle sizer

    NASA Astrophysics Data System (ADS)

    Lefebvre, F.; Petit, J.; Nassar, G.; Debreyne, P.; Delaplace, G.; Nongaillard, B.

    2013-07-01

    This paper reports the development of a new method of particle sizing in a liquid. This method uses high frequency focused ultrasounds to detect particles crossing the focal zone of an ultrasonic sensor and to determine their size distribution by processing the reflected echoes. The major advantage of this technique compared to optical sizing methods is its ability to measure the size of particles suspended in an opaque liquid without any dedicated sample preparation. Validations of ultrasonic measurements were achieved on suspensions of polymethyl methacrylate beads in a size range extending from a few micrometer to several hundred micrometer with a temporal resolution of 1 s. The inline detection of aggregate formation was also demonstrated.

  16. High frequency electromagnetic response of the moon

    NASA Technical Reports Server (NTRS)

    Schubert, G.; Schwartz, K.

    1971-01-01

    It is shown that the contribution of higher harmonics to the lunar transfer functions for the tangential components of the surface magnetic field is significant at frequencies greater than 0.01 Hz. The inclusion of the higher harmonics shows that there are two distinct transfer functions corresponding to the components of the tangential surface magnetic field perpendicular and parallel to the direction of the wave vector of the external disturbance forcing the lunar induction. The dependences of these transfer functions on frequency and location are determined. The effects of the higher harmonics can: (1) account for a hitherto unexplained feature in the Apollo 12-Explorer 35 transfer functions, namely the rolloff at high frequencies; and (2) offer a possible explanation for the frequency dependence of the difference between the transfer functions for the two orthogonal components of the surface magnetic field. The harmonic response of a simple current layer model of the moon is derived.

  17. High-frequency resonant-tunneling oscillators

    NASA Technical Reports Server (NTRS)

    Brown, E. R.; Parker, C. D.; Calawa, A. R.; Manfra, M. J.; Chen, C. L.

    1991-01-01

    Advances in high-frequency resonant-tunneling-diode (RTD) oscillators are described. Oscillations up to a frequency of 420 GHz have been achieved in the GaAs/AlAs system. Recent results obtained with In0.53Ga0.47As/AlAs and InAs/AlSb RTDs show a greatly increased power density and indicate the potential for fundamental oscillations up to about 1 THz. These results are consistent with a lumped-element equivalent circuit model of the RTD. The model shows that the maximum oscillation frequency of the GaAs/AlAs RTDs is limited primarily by series resistance, and that the power density is limited by low peak-to-valley current ratio.

  18. The linkage between marine sediment records and changes in Holocene Saharan landscape: simulating the dust cycle

    NASA Astrophysics Data System (ADS)

    Egerer, Sabine; Claussen, Martin; Reick, Christian; Stanelle, Tanja

    2016-04-01

    Marine sediment records reveal an abrupt and strong increase in dust deposition in the North Atlantic at the end of the African Humid Period about 4.9 ka to 5.5 ka ago (deMenocal et al., 2000; McGee et al., 2013). The change in dust flux has been attributed to varying Saharan land surface cover. Alternatively, the enhanced dust accumulation is linked to enhanced surface winds and a consequent intensification of coastal upwelling. We present simulation results from a recent sensitivity study, where we demonstrate for the first time the direct link between dust accumulation in marine cores and changes in Saharan land surface during the Holocene. We have simulated timeslices of he mid-Holocene (6 ka BP) and pre-industrial (1850 AD) dust cycle as a function of Saharan land surface cover and atmosphere-ocean conditions using the coupled atmosphere-aerosol model ECHAM6.1-HAM2.1. We prescribe mid-Holocene vegetation cover based on a vegetation reconstruction from pollen data (Hoelzmann et al., 1998) and mid-Holocene lake surface area is determined using a water routing and storage model (Tegen et al., 2002). In agreement with data from marine sediment cores, our simulations show that mid-Holocene dust deposition fluxes in the North Atlantic were two to three times lower compared with pre-industrial fluxes. We identify Saharan land surface characteristics to be the main control on dust transport from North Africa to the North Atlantic. We conclude that the variation in dust accumulation in marine cores is likely related to a transition of the Saharan landscape during the Holocene and not due to changes in atmospheric or ocean conditions alone. Reference: deMenocal, P., Ortiz, J., Guilderson, T., Adkins, J., Sarnthein, M., Baker, L., and Yarusinsky, M.: Abrupt onset and termination of the African Humid Period:: rapid climate responses to gradual insolation forcing, Quaternary Science Reviews, 19, 347-361, 2000. Hoelzmann, P., Jolly, D., Harrison, S. P., Laarif, F

  19. Seasonal cycles in biogenic production and export in Northern Bay of Bengal sediment traps

    NASA Astrophysics Data System (ADS)

    Stoll, Heather M.; Arevalos, Alicia; Burke, Andrea; Ziveri, Patrizia; Mortyn, Graham; Shimizu, Nobumichi; Unger, Daniela

    2007-03-01

    Sediment traps in the northern and north central Bay of Bengal are characterized by highly seasonal fluxes and unusually high efficiency of organic carbon export. However, the mechanism for high export production remains under debate. To evaluate the relationships between production in the photic zone and export processes responsible for the fluxes into the traps, over an annual cycle we examine a series of indicators of production regime set in surface waters. These indicators include communities of planktic foraminifera and coccolithophores, stable isotopic chemistry of foraminifera and coccoliths, and the Sr/Ca ratios in coccoliths. Coccolith and foraminiferal assemblages confirm that the Bay of Bengal is a region of high productivity. Coccolithophore communities are dominated to an unusually high degree (90%) by the lower photic zone dweller Florisphaera profunda, a species adapted to high-nutrient and low-light conditions typical of stratified waters like those induced by the strong halocline in the Bay of Bengal. Cyclonic eddy pumping and strong winds during the southwest monsoon (SWM) increase the relative abundance of upwelling indicator species like foraminifera Globigerina bulloides and the upper photic coccolithophores Globigerina oceanica and Emiliania huxleyi. However, while upwelling and eddy pumping do coincide with high opal and coccolith export, in both traps peak organic carbon export precedes the onset of eddy pumping and upwelling indicators. These data suggest an alternate mode of production in the Bay of Bengal, which is not driven by upwelling but rather high production deeper in the water column, probably by taxa adapted to lower light levels. In both traps, the pulses of organic carbon export coincide with elevated fluxes of planktonic foraminifera, which likely reflect increased primary production. Consequently, while major export pulses of organic carbon coincide with pulses of lithogenic export, the pulses of organic carbon export are

  20. Effects of conventional and biodegradable microplastics on a marine ecosystem engineer (Arenicola marina) and sediment nutrient cycling.

    PubMed

    Green, Dannielle Senga; Boots, Bas; Sigwart, Julia; Jiang, Shan; Rocha, Carlos

    2016-01-01

    Effects of microplastic pollution on benthic organisms and ecosystem services provided by sedimentary habitats are largely unknown. An outdoor mesocosm experiment was done to realistically assess the effects of three different types of microplastic pollution (one biodegradable type; polylactic acid and two conventional types; polyethylene and polyvinylchloride) at increasing concentrations (0.02, 0.2 and 2% of wet sediment weight) on the health and biological activity of lugworms, Arenicola marina (Linnaeus, 1758), and on nitrogen cycling and primary productivity of the sediment they inhabit. After 31 days, A. marina produced less casts in sediments containing microplastics. Metabolic rates of A. marina increased, while microalgal biomass decreased at high concentrations, compared to sediments with low concentrations or without microplastics. Responses were strongest to polyvinylchloride, emphasising that different materials may have differential effects. Each material needs to be carefully evaluated in order to assess their risks as microplastic pollution. Overall, both conventional and biodegradable microplastics in sandy sediments can affect the health and behaviour of lugworms and directly or indirectly reduce primary productivity of these habitats. PMID:26552519

  1. Corrosion monitoring using high-frequency guided ultrasonic waves

    NASA Astrophysics Data System (ADS)

    Fromme, Paul

    2014-02-01

    Corrosion develops due to adverse environmental conditions during the life cycle of a range of industrial structures, e.g., offshore oil platforms, ships, and desalination plants. Both pitting corrosion and generalized corrosion leading to wall thickness loss can cause the degradation of the structural integrity. The nondestructive detection and monitoring of corrosion damage in difficult to access areas can be achieved using high frequency guided waves propagating along the structure from accessible areas. Using standard ultrasonic transducers with single sided access to the structure, guided wave modes were generated that penetrate through the complete thickness of the structure. The wave propagation and interference of the different guided wave modes depends on the thickness of the structure. Laboratory experiments were conducted and the wall thickness reduced by consecutive milling of the steel structure. Further measurements were conducted using accelerated corrosion in a salt water bath and the damage severity monitored. From the measured signal change due to the wave mode interference the wall thickness reduction was monitored. The high frequency guided waves have the potential for corrosion damage monitoring at critical and difficult to access locations from a stand-off distance.

  2. Corrosion monitoring using high-frequency guided waves

    NASA Astrophysics Data System (ADS)

    Fromme, P.

    2016-04-01

    Corrosion can develop due to adverse environmental conditions during the life cycle of a range of industrial structures, e.g., offshore oil platforms, ships, and desalination plants. Generalized corrosion leading to wall thickness loss can cause the reduction of the strength and thus degradation of the structural integrity. The monitoring of corrosion damage in difficult to access areas can be achieved using high frequency guided waves propagating along the structure from accessible areas. Using standard ultrasonic wedge transducers with single sided access to the structure, guided wave modes were selectively generated that penetrate through the complete thickness of the structure. The wave propagation and interference of the different guided wave modes depends on the thickness of the structure. Laboratory experiments were conducted for wall thickness reduction due to milling of the steel structure. From the measured signal changes due to the wave mode interference the reduced wall thickness was monitored. Good agreement with theoretical predictions was achieved. The high frequency guided waves have the potential for corrosion damage monitoring at critical and difficult to access locations from a stand-off distance.

  3. Community genomic analyses constrain the distribution of metabolic traits across the Chloroflexi phylum and indicate roles in sediment carbon cycling

    PubMed Central

    2013-01-01

    Background Sediments are massive reservoirs of carbon compounds and host a large fraction of microbial life. Microorganisms within terrestrial aquifer sediments control buried organic carbon turnover, degrade organic contaminants, and impact drinking water quality. Recent 16S rRNA gene profiling indicates that members of the bacterial phylum Chloroflexi are common in sediment. Only the role of the class Dehalococcoidia, which degrade halogenated solvents, is well understood. Genomic sampling is available for only six of the approximate 30 Chloroflexi classes, so little is known about the phylogenetic distribution of reductive dehalogenation or about the broader metabolic characteristics of Chloroflexi in sediment. Results We used metagenomics to directly evaluate the metabolic potential and diversity of Chloroflexi in aquifer sediments. We sampled genomic sequence from 86 Chloroflexi representing 15 distinct lineages, including members of eight classes previously characterized only by 16S rRNA sequences. Unlike in the Dehalococcoidia, genes for organohalide respiration are rare within the Chloroflexi genomes sampled here. Near-complete genomes were reconstructed for three Chloroflexi. One, a member of an unsequenced lineage in the Anaerolinea, is an aerobe with the potential for respiring diverse carbon compounds. The others represent two genomically unsampled classes sibling to the Dehalococcoidia, and are anaerobes likely involved in sugar and plant-derived-compound degradation to acetate. Both fix CO2 via the Wood-Ljungdahl pathway, a pathway not previously documented in Chloroflexi. The genomes each encode unique traits apparently acquired from Archaea, including mechanisms of motility and ATP synthesis. Conclusions Chloroflexi in the aquifer sediments are abundant and highly diverse. Genomic analyses provide new evolutionary boundaries for obligate organohalide respiration. We expand the potential roles of Chloroflexi in sediment carbon cycling beyond

  4. Interactions Between Microbial Iron Reduction and Metal Geochemistry: Effect of Redox Cycling on Transition Metal Speciation in Iron Bearing Sediments

    SciTech Connect

    D. Craig Cooper; Flynn W. Picardal; Aaron J. Coby

    2006-02-01

    Microbial iron reduction is an important biogeochemical process that can affect metal geochemistry in sediments through direct and indirect mechanisms. With respect to Fe(III) (hydr)oxides bearing sorbed divalent metals, recent reports have indicated that (1) microbial reduction of goethite/ferrihydrite mixtures preferentially removes ferrihydrite, (2) this process can incorporate previously sorbed Zn(II) into an authigenic crystalline phase that is insoluble in 0.5 M HCl, (3) this new phase is probably goethite, and (4) the presence of nonreducible minerals can inhibit this transformation. This study demonstrates that a range of sorbed transition metals can be selectively sequestered into a 0.5 M HCl insoluble phase and that the process can be stimulated through sequential steps of microbial iron reduction and air oxidation. Microbial reduction experiments with divalent Cd, Co, Mn, Ni, Pb, and Zn indicate that all metals save Mn experienced some sequestration, with the degree of metal incorporation into the 0.5 M HCl insoluble phase correlating positively with crystalline ionic radius at coordination number = 6. Redox cycling experiments with Zn adsorbed to synthetic goethite/ferrihydrite or iron-bearing natural sediments indicate that redox cycling from iron reducing to iron oxidizing conditions sequesters more Zn within authigenic minerals than microbial iron reduction alone. In addition, the process is more effective in goethite/ferrihydrite mixtures than in iron-bearing natural sediments. Microbial reduction alone resulted in a ~3× increase in 0.5 M HCl insoluble Zn and increased aqueous Zn (Zn-aq) in goethite/ferrihydrite, but did not significantly affect Zn speciation in natural sediments. Redox cycling enhanced the Zn sequestration by ~12% in both goethite/ferrihydrite and natural sediments and reduced Zn-aq to levels equal to the uninoculated control in goethite/ferrihydrite and less than the uninoculated control in natural sediments. These data suggest

  5. Biostimulation of Iron Reduction and Subsequent Oxidation of Sediment Containing Fe-silicates and Fe-oxides: Effect of Redox Cycling on Fe(III) Bioreduction

    SciTech Connect

    Komlos, John; Kukkadapu, Ravi K.; Zachara, John M.; Jaffe, Peter R.

    2007-07-01

    Microbial reduction of iron has been shown to be important in the transformation and remediation of contaminated sediments. Re-oxidation of microbially reduced iron may occur in sediments that experience oxidation-reduction cycling and can thus impact the extent of contaminant remediation. The purpose of this research was to quantify iron oxidation in a flow-through column filled with biologically-reduced sediment and to compare the iron phases in the re-oxidized sediment to both the pristine and biologically-reduced sediment. The sediment contained both Fe(III)-oxides (primarily goethite) and silicate Fe (illite/vermiculite) and was biologically reduced in phosphate buffered (PB) medium during a 497 day column experiment with acetate supplied as the electron donor. Long-term iron reduction resulted in partial reduction of silicate Fe(III) without any goethite reduction, based on Mössbauer spectroscopy measurements. This reduced sediment was treated with an oxygenated PB solution in a flow-through column resulting in the oxidation of 38% of the biogenic Fe(II). Additional batch experiments showed that the Fe(III) in the oxidized sediment was more quickly reduced compared to the pristine sediment, indicating that oxidation of the sediment not only regenerated Fe(III) but also enhanced iron reduction compared to the pristine sediment. Oxidation-reduction cycling may be a viable method to extend iron-reducing conditions during in-situ bioremediation.

  6. Active Control of High-Frequency Combustor Instability Demonstrated

    NASA Technical Reports Server (NTRS)

    DeLaat, John C.; Chang, Clarence T.

    2003-01-01

    To reduce the environmental impact of aerospace propulsion systems, extensive research is being done in the development of lean-burning (low fuel-to-air ratio) combustors that can reduce emissions throughout the mission cycle. However, these lean-burning combustors have an increased susceptibility to thermoacoustic instabilities-high-pressure oscillations much like sound waves that can cause severe high-frequency vibrations in the combustor. These pressure waves can fatigue the combustor components and even the downstream turbine blades. This can significantly decrease the combustor and turbine safe operating life. Thus, suppression of the thermoacoustic combustor instabilities is an enabling technology for lean, low-emissions combustors. Under the Propulsion and Power Program, the NASA Glenn Research Center in partnership with Pratt & Whitney, United Technologies Research Center, and Georgia Institute of Technology is developing technologies for the active control of combustion instabilities.

  7. Specimen Design for Fatigue Testing at Very High Frequencies

    NASA Astrophysics Data System (ADS)

    MATIKAS, T. E.

    2001-11-01

    Components in rotational machinery such as turbine blades used in military aircraft engines are subjected to low-amplitude, high-frequency loads in the kHz range. Under high cycle fatigue (HCF), the initiation state of a crack consumes most of the life of the component. Vibratory stresses may therefore result in unexpected failures of the material. Hence, there is a need for HCF studies to address HCF-related failures of turbine engines and to develop a life prediction methodology. Ultrasonic fatigue provides accelerated HCF testing enabling the simulation of realistic loading conditions for testing materials used in structural components subjected to vibratory stresses. Specimen design is critical for optimum ultrasonic fatigue testing. The objective of this study is therefore to develop analytical modelling necessary for the design of test coupons to be fatigue tested at ultrasonic frequencies.

  8. Structure and diversity of functional guilds in the microbial nitrogen cycle of estuarine sediments

    NASA Astrophysics Data System (ADS)

    Ward, B. B.; Francis, C. A.; Taroncher-Oldenburg, G.; Cornwell, J.

    2002-12-01

    Denitrification is a major flux of nitrogen in Chesapeake Bay, an estuary with a long residence time and high organic and inorganic nutrient inputs from the large surrounding watershed. The estuarine system spans a complex gradient of salinity and many correlated chemical constituents, from its upper bay and river freshwater end members to its nearly full strength seawater lower Bay. Denitrification rates in sediments, computed from net nitrogen fluxes in simulated in situ core incubations, show distinct reproducible patterns along the Bay. Highest rates are observed in sediments from the low salinity, high nitrate upper stations in the Bay and Choptank River. Lower rates occur in the low nitrate, oxygen depleted mid bay sediments and in the metabolically less active south Bay sediments. Gene sequences for nitrite reductase, the key enzyme in denitrification, show very high diversity in Bay and River sediments. On the basis of clone library sequences alone, however, there are distinct clades and patterns indicating highest diversity in the upper Bay and River sediments and lower diversity in the lower Bay sediments. Using a DNA microarray containing many individual nitrite reductase sequences, we investigated the population structure of denitrification genes along the estuarine gradient. Evaluation of gene expression patterns, in addition to presence/absence or abundance of individual genes, will allow a direct assessment of the links between diversity and biogeochemical transformation rates for particular functional guilds. The rate of denitrification and its regulation by environmental variables may be reflected in patterns of guild composition and activity.

  9. Accelerated Rates of Nitrogen Cycling and N2O Production in Salt Marsh Sediments due to Long-Term Fertilization

    NASA Astrophysics Data System (ADS)

    Peng, X.; Ji, Q.; Angell, J.; Kearns, P.; Bowen, J. L.; Ward, B. B.

    2014-12-01

    Intensified sedimentary production of nitrous oxide (N2O), one of the most potent greenhouse gases, is one of the many possible environmental consequences of elevated nitrogen (N) loading into estuarine ecosystems. This study investigates the response to over 40 years of fertilization of nitrogen removal processes in the sediments of the Great Sippewissett Marsh in Falmouth, MA. Sediment slurries were incubated (1.5 hr) with trace amounts (< 10% of ambient concentration) of 15NH4+ + 14NO3- or 15NO3- + 14NH4+. An additional parallel incubation with 15NH4+ + 14NO3- and 1 mM of allylthiourea (ATU) was included to measure rates of anaerobic ammonia oxidation (anammox). Well-homogenized slurries filled about 10% of the volume in the gas-tight incubation vials, and the rest of the volume was replaced with an O2/He (20%/80%) mixture. The production of 29N2, 44N2O and 45N2O were determined using isotope ratio mass spectrometry. The rate of total N2O production in fertilized sediments (0.89 nmol hr-1 g-1 wet weight) was 30-fold higher than in unfertilized sediments. The ratio of N2O to N2 production was also significantly higher in fertilized sediments (2.9%) than in unfertilized sediments (1.2%). This highlights the disproportionally large effect of long-term fertilization on N2O production in salt marsh sediments. The reduced oxygen level and higher ammonium concentrations in situ probably contributed to the significant rise in N2O production as a result of long-term fertilization. When detected, anammox and coupled nitrification-denitrification accounted for 10% and 14% of the total N2 production in fertilized sediments (30.5 nmol hr-1 g-1 wet weight), respectively, whereas neither was detected in unfertilized sediments. Thus these experiments indicate that N loading has important effects on multiple N cycle processes that result in N loss and N2O production.

  10. Linking sediment structure, hydrological functioning and biogeochemical cycling in disturbed coastal saltmarshes and implications for vegetation development

    NASA Astrophysics Data System (ADS)

    Spencer, Kate; Harvey, Gemma; James, Tempest; Simon, Carr; Michelle, Morris

    2014-05-01

    with preferential horizontal flows. The undisturbed saltmarsh displayed typical vertical geochemical sediment profiles. However, in the restored sites total Fe and Mn are elevated at depth indicating an absence of diagenetic cycling, whilst porewater sulphate and nitrate increased at depth suggesting that vertical solute transport is impeded in restored sites. In surface sediments, though total Hg concentrations are similar, Hg methylation rates are significantly higher than in the undisturbed saltmarsh suggesting that surface anoxia and poor drainage may result in increased mobilization and bioavailability of Hg. These findings have implications for the wider biogeochemical ecosystem services offered by saltmarsh restoration and the water-logged, anoxic conditions produced are unsuitable for seedling germination and plant growth. This highlights the need for integrated understanding of physical and biogeochemical processes.

  11. Aerodynamics of high frequency flapping wings

    NASA Astrophysics Data System (ADS)

    Hu, Zheng; Roll, Jesse; Cheng, Bo; Deng, Xinyan

    2010-11-01

    We investigated the aerodynamic performance of high frequency flapping wings using a 2.5 gram robotic insect mechanism developed in our lab. The mechanism flaps up to 65Hz with a pair of man-made wing mounted with 10cm wingtip-to-wingtip span. The mean aerodynamic lift force was measured by a lever platform, and the flow velocity and vorticity were measured using a stereo DPIV system in the frontal, parasagittal, and horizontal planes. Both near field (leading edge vortex) and far field flow (induced flow) were measured with instantaneous and phase-averaged results. Systematic experiments were performed on the man-made wings, cicada and hawk moth wings due to their similar size, frequency and Reynolds number. For insect wings, we used both dry and freshly-cut wings. The aerodynamic force increase with flapping frequency and the man-made wing generates more than 4 grams of lift at 35Hz with 3 volt input. Here we present the experimental results and the major differences in their aerodynamic performances.

  12. High frequency, high power capacitor development

    NASA Technical Reports Server (NTRS)

    White, C. W.; Hoffman, P. S.

    1983-01-01

    A program to develop a special high energy density, high power transfer capacitor to operate at frequency of 40 kHz, 600 V rms at 125 A rms plus 600 V dc bias for space operation. The program included material evaluation and selection, a capacitor design was prepared, a thermal analysis performed on the design. Fifty capacitors were manufactured for testing at 10 kHz and 40 kHz for 50 hours at Industrial Electric Heating Co. of Columbus, Ohio. The vacuum endurance test used on environmental chamber and temperature plate furnished by Maxwell. The capacitors were energized with a special power conditioning apparatus developed by Industrial Electric Heating Co. Temperature conditions of the capacitors were monitored by IEHCo test equipment. Successful completion of the vacuum endurance test series confirmed achievement of the main goal of producing a capacitor or reliable operation at high frequency in an environment normally not hospitable to electrical and electronic components. The capacitor developed compared to a typical commercial capacitor at the 40 kHz level represents a decrease in size and weight by a factor of seven.

  13. A high frequency electromagnetic impedance imaging system

    SciTech Connect

    Tseng, Hung-Wen; Lee, Ki Ha; Becker, Alex

    2003-01-15

    Non-invasive, high resolution geophysical mapping of the shallow subsurface is necessary for delineation of buried hazardous wastes, detecting unexploded ordinance, verifying and monitoring of containment or moisture contents, and other environmental applications. Electromagnetic (EM) techniques can be used for this purpose since electrical conductivity and dielectric permittivity are representative of the subsurface media. Measurements in the EM frequency band between 1 and 100 MHz are very important for such applications, because the induction number of many targets is small and the ability to determine the subsurface distribution of both electrical properties is required. Earlier workers were successful in developing systems for detecting anomalous areas, but quantitative interpretation of the data was difficult. Accurate measurements are necessary, but difficult to achieve for high-resolution imaging of the subsurface. We are developing a broadband non-invasive method for accurately mapping the electrical conductivity and dielectric permittivity of the shallow subsurface using an EM impedance approach similar to the MT exploration technique. Electric and magnetic sensors were tested to ensure that stray EM scattering is minimized and the quality of the data collected with the high-frequency impedance (HFI) system is good enough to allow high-resolution, multi-dimensional imaging of hidden targets. Additional efforts are being made to modify and further develop existing sensors and transmitters to improve the imaging capability and data acquisition efficiency.

  14. A High Frequency Model of Cascade Noise

    NASA Technical Reports Server (NTRS)

    Envia, Edmane

    1998-01-01

    Closed form asymptotic expressions for computing high frequency noise generated by an annular cascade in an infinite duct containing a uniform flow are presented. There are two new elements in this work. First, the annular duct mode representation does not rely on the often-used Bessel function expansion resulting in simpler expressions for both the radial eigenvalues and eigenfunctions of the duct. In particular, the new representation provides an explicit approximate formula for the radial eigenvalues obviating the need for solutions of the transcendental annular duct eigenvalue equation. Also, the radial eigenfunctions are represented in terms of exponentials eliminating the numerical problems associated with generating the Bessel functions on a computer. The second new element is the construction of an unsteady response model for an annular cascade. The new construction satisfies the boundary conditions on both the cascade and duct walls simultaneously adding a new level of realism to the noise calculations. Preliminary results which demonstrate the effectiveness of the new elements are presented. A discussion of the utility of the asymptotic formulas for calculating cascade discrete tone as well as broadband noise is also included.

  15. High-frequency graphene voltage amplifier.

    PubMed

    Han, Shu-Jen; Jenkins, Keith A; Valdes Garcia, Alberto; Franklin, Aaron D; Bol, Ageeth A; Haensch, Wilfried

    2011-09-14

    While graphene transistors have proven capable of delivering gigahertz-range cutoff frequencies, applying the devices to RF circuits has been largely hindered by the lack of current saturation in the zero band gap graphene. Herein, the first high-frequency voltage amplifier is demonstrated using large-area chemical vapor deposition grown graphene. The graphene field-effect transistor (GFET) has a 6-finger gate design with gate length of 500 nm. The graphene common-source amplifier exhibits ∼5 dB low frequency gain with the 3 dB bandwidth greater than 6 GHz. This first AC voltage gain demonstration of a GFET is attributed to the clear current saturation in the device, which is enabled by an ultrathin gate dielectric (4 nm HfO(2)) of the embedded gate structures. The device also shows extrinsic transconductance of 1.2 mS/μm at 1 V drain bias, the highest for graphene FETs using large-scale graphene reported to date. PMID:21805988

  16. High-Frequency Observations of Blazars

    NASA Technical Reports Server (NTRS)

    Marscher, A. P.; Marchenko-Jorstad, S. G.; Mattox, J. R.; Wehrle, A. E.; Aller, M. F.

    2000-01-01

    We report on the results of high-frequency VLBA observations of 42 gamma-ray bright blazars monitored at 22 and 43 GHz between 1993.9 and 1997.6. In 1997 the observations included polarization-sensitive imaging. The cores of gamma-ray blazars are only weakly polarized, with EVPAs (electric-vector position angles) usually within 40 deg of the local direction of the jet. The EVPAs of the jet components are usually within 20 deg of the local jet direction. The apparent speeds of the gamma-ray bright blazars are considerably faster than in the general population of bright compact radio sources. Two X-ray flares (observed with RXTE) of the quasar PKS 1510-089 appear to be related to radio flares, but with the radio leading the X-ray variations by about 2 weeks. This can be explained either by synchrotron self-Compton emission in a component whose variations are limited by light travel time or by the Mirror Compton model.

  17. High-frequency plasma-heating apparatus

    DOEpatents

    Brambilla, Marco; Lallia, Pascal

    1978-01-01

    An array of adjacent wave guides feed high-frequency energy into a vacuum chamber in which a toroidal plasma is confined by a magnetic field, the wave guide array being located between two toroidal current windings. Waves are excited in the wave guide at a frequency substantially equal to the lower frequency hybrid wave of the plasma and a substantially equal phase shift is provided from one guide to the next between the waves therein. For plasmas of low peripheral density gradient, the guides are excited in the TE.sub.01 mode and the output electric field is parallel to the direction of the toroidal magnetic field. For exciting waves in plasmas of high peripheral density gradient, the guides are excited in the TM.sub.01 mode and the magnetic field at the wave guide outlets is parallel to the direction of the toroidal magnetic field. The wave excited at the outlet of the wave guide array is a progressive wave propagating in the direction opposite to that of the toroidal current and is, therefore, not absorbed by so-called "runaway" electrons.

  18. Mercury cycling in stream ecosystems. 2. Benthic methylmercury production and bed sediment - Pore water partitioning

    USGS Publications Warehouse

    Marvin-DiPasquale, M.; Lutz, M.A.; Brigham, M.E.; Krabbenhoft, D.P.; Aiken, G.R.; Orem, W.H.; Hall, B.D.

    2009-01-01

    Mercury speciation, controls on methylmercury (MeHg) production, and bed sediment - pore water partitioning of total Hg (THg) and MeHg were examined in bed sediment from eight geochemically diverse streams where atmospheric deposition was the predominant Hg input. Across all streams, sediment THg concentrations were best described as a combined function of sediment percent fines (%fines; particles < 63 ??m) and organic content. MeHg concentrations were best described as a combined function of organic content and the activity of the Hg(II)-methylating microbial community and were comparable to MeHg concentrations in streams with Hg inputs from industrial and mining sources. Whole sediment tin-reducible inorganic reactive Hg (Hg(II)R) was used as a proxy measure for the Hg(II) pool available for microbial methylation. In conjunction with radiotracer-derived rate constants of 203Hg(II) methylation, Hg(II)R was used to calculate MeHg production potential rates and to explain the spatial variability in MeHg concentration. The %Hg(II)R (of THg) was low (2.1 ?? 5.7%) and was inversely related to both microbial sulfate reduction rates and sediment total reduced sulfur concentration. While sediment THg concentrations were higher in urban streams, %MeHg and %Hg(II)R were higher in nonurban streams. Sediment pore water distribution coefficients (log Kd's) for both THg and MeHg were inversely related to the log-transformed ratio of pore water dissolved organic carbon (DOC) to bed sediment %fines. The stream with the highest drainage basin wetland density also had the highest pore water DOC ?? 2009 American Chemical Society.

  19. The nature of high frequency sister chromatid exchange cells (HFCs).

    PubMed

    Ponzanelli, I; Landi, S; Bernacchi, F; Barale, R

    1997-09-01

    We employed the three-way differential staining technique (TWD), which allows SCEs to be distinguished on a per generation basis by scoring third metaphases (M3), in order to study the spontaneous levels of SCEs in normal and high frequency cells (HFCs) that occurred in the first (S1), second (S2) and third (S3) S phases. Fifty one of 900 lymphocytes from 37 healthy donors were defined as HFCs by calculating the 95th percentile of the distribution of SCEs in S1 + S2. 'Normal' cells presented almost the same number of SCEs after the first, second and third cell cycles (SCE averages of 2.43, 2.04 and 3.53 respectively). In contrast, HFCs showed a higher SCE count in S1, which decreased rapidly through the cycles and reached baseline level at S3 (SCE averages of 7.18, 4.29 and 3.45 respectively). This would suggest that the lesions responsible for the higher SCE frequency in HFCs were effectively removed after two cell cycles and strongly support the hypothesis that HFCs are lymphocytes which accumulate higher levels of DNA lesions through time. PMID:9379910

  20. Analysis of high frequency geostationary ocean colour data using DINEOF

    NASA Astrophysics Data System (ADS)

    Alvera-Azcárate, Aida; Vanhellemont, Quinten; Ruddick, Kevin; Barth, Alexander; Beckers, Jean-Marie

    2015-06-01

    DINEOF (Data Interpolating Empirical Orthogonal Functions), a technique to reconstruct missing data, is applied to turbidity data obtained through the Spinning Enhanced Visible and Infrared Imager (SEVIRI) on board Meteosat Second Generation 2. The aim of this work is to assess if the tidal variability of the southern North Sea in 2008 can be accurately reproduced in the reconstructed dataset. Such high frequency data have not previously been analysed with DINEOF and present new challenges, like a strong tidal signal and long night-time gaps. An outlier detection approach that exploits the high temporal resolution (15 min) of the SEVIRI dataset is developed. After removal of outliers, the turbidity dataset is reconstructed with DINEOF. In situ Smartbuoy data are used to assess the accuracy of the reconstruction. Then, a series of tidal cycles are examined at various positions over the southern North Sea. These examples demonstrate the capability of DINEOF to reproduce tidal variability in the reconstructed dataset, and show the high temporal and spatial variability of turbidity in the southern North Sea. An analysis of the main harmonic constituents (annual cycle, daily cycle, M2 and S2 tidal components) is performed, to assess the contribution of each of these modes to the total variability of turbidity. The variability not explained by the harmonic fit, due to the natural processes and satellite processing errors as noise, is also assessed.

  1. Anaerobic oxidation of short-chain alkanes in hydrothermal sediments: potential influences on sulfur cycling and microbial diversity

    SciTech Connect

    Adams, MM; Hoarfrost, AL; Bose, A; Joye, SB; Girguis, PR

    2013-05-14

    Short-chain alkanes play a substantial role in carbon and sulfur cycling at hydrocarbon-rich environments globally, yet few studies have examined the metabolism of ethane (C-2), propane (C-3), and butane (C-4) in anoxic sediments in contrast to methane (C-1). In hydrothermal vent systems, short-chain alkanes are formed over relatively short geological time scales via thermogenic processes and often exist at high concentrations. The sediment-covered hydrothermal vent systems at Middle Valley (MV Juan de Fuca Ridge) are an ideal site for investigating the anaerobic oxidation of C-1-C-4 alkanes, given the elevated temperatures and dissolved hydrocarbon species characteristic of these metalliferous sediments. We examined whether MV microbial communities oxidized C-1-C-4 alkanes under mesophilic to thermophilic sulfate-reducing conditions. Here we present data from discrete temperature (25, 55, and 75 degrees C) anaerobic batch reactor incubations of MV sediments supplemented with individual alkanes. Co-registered alkane consumption and sulfate reduction (SR) measurements provide clear evidence for C-1-C-4 alkane oxidation linked to SR over time and across temperatures. In these anaerobic batch reactor sediments, 16S ribosomal RNA pyrosequencing revealed that Deltaproteobacteria, particularly a novel sulfate-reducing lineage, were the likely phylotypes mediating the oxidation of C-2-C-4 alkanes. Maximum C-1-C-4 alkane oxidation rates occurred at 55 degrees C, which reflects the mid-core sediment temperature profile and corroborates previous studies of rate maxima for the anaerobic oxidation of methane (AOM). Of the alkanes investigated, C-3 was oxidized at the highest rate over time, then C-4, C-2, and C-1, respectively. The implications of these results are discussed with respect to the potential competition between the anaerobic oxidation of C-2-C(4)alkanes with AOM for available oxidants and the influence on the fate of C-1 derived from these hydrothermal systems.

  2. Effects of Deepwater Algae on C-N-P Cycling in Permeable Sediments

    NASA Astrophysics Data System (ADS)

    Sansone, F. J.; Spalding, H. L.; Smith, C. M.

    2007-12-01

    Extensive deepwater (50-100 m depth) meadows of native and introduced macroalgae occur offshore of the main Hawaiian Islands. Sediment porewater (to 25 cm sediment depth) and overlying seawater were collected during September and December 2004, and November and December 2006 from 13 vegetated and non-vegetated deepwater sites on sand and muddy-sand seafloors offshore from Oahu to Kaho'olawe. Porewater dissolved nutrient concentrations were comparable to those in nearshore permeable (sandy) sediments, with highly elevated concentrations at sediment depths available to algal holdfasts. Maximum observed concentrations were: 2.3 uM phosphate, 30 uM nitrate, 0.65 uM nitrite, 105 uM ammonium, and 88 uM silica. The sediment organic matter content was not correlated with the presence or absence of macroalgae. Vegetated sediments were consistently more oxidizing than non-vegetated sediments, judged from (nitrate + nitrite)/ammonium ratios. Halimeda-vegetated sediments had low DIN levels compared to other sites, suggesting algal uptake. These sands also exhibited dissolved inorganic carbon depletion at depth, perhaps also related to algal metabolism. In contrast, Udotea-vegetated sediments had high levels of DIN, indicating little or no algal DIN uptake and/or the presence of active N-fixation; these sands also had no DIC depletion at depth. Udotea-vegetated sediments also appeared highly oxidizing, evidenced by very high levels of dissolved nitrate and nitrite, along with low levels of ammonium. The calculated tracer N* was used to verify the conclusions regarding net DIN uptake; a similar calculated tracer, computed using Redfield-type N:Si ratios, was used to confirm the N* results. Unvegetated, nutrient- and organic-rich sandy mud along the flanks of Haleakala volcano showed near complete dissolved phosphate removal, perhaps due to adsorption onto freshly weathered iron oxy-hydroxides. Such phosphate removal was not seen at other sites, including a nearby non

  3. The Effect of Nitrogen Enrichment on C1-Cycling Microorganisms and Methane Flux in Salt Marsh Sediments

    PubMed Central

    Irvine, Irina C.; Vivanco, Lucía; Bentley, Peris N.; Martiny, Jennifer B. H.

    2012-01-01

    Methane (CH4) flux from ecosystems is driven by C1-cycling microorganisms – the methanogens and the methylotrophs. Little is understood about what regulates these communities, complicating predictions about how global change drivers such as nitrogen enrichment will affect methane cycling. Using a nitrogen addition gradient experiment in three Southern California salt marshes, we show that sediment CH4 flux increased linearly with increasing nitrogen addition (1.23 μg CH4 m−2 day−1 for each g N m−2 year−1 applied) after 7 months of fertilization. To test the reason behind this increased CH4 flux, we conducted a microcosm experiment altering both nitrogen and carbon availability under aerobic and anaerobic conditions. Methanogenesis appeared to be both nitrogen and carbon (acetate) limited. N and C each increased methanogenesis by 18%, and together by 44%. In contrast, methanotrophy was stimulated by carbon (methane) addition (830%), but was unchanged by nitrogen addition. Sequence analysis of the sediment methylotroph community with the methanol dehydrogenase gene (mxaF) revealed three distinct clades that fall outside of known lineages. However, in agreement with the microcosm results, methylotroph abundance (assayed by qPCR) and composition (assayed by terminal restriction fragment length polymorphism analysis) did not vary across the experimental nitrogen gradient in the field. Together, these results suggest that nitrogen enrichment to salt marsh sediments increases methane flux by stimulating the methanogen community. PMID:22470369

  4. The Effects of Zebra Mussels on Nitrogen Cycling and Sediment Physiochemical Characteristics: A Laboratory Experiment.

    NASA Astrophysics Data System (ADS)

    Bruesewitz, D. A.; Tank, J. L.; Bernot, M. J.

    2005-05-01

    Many ecosystems experiencing anthropogenic nitrogen loading have also been invaded by zebra mussels (ZM), therefore it is important to understand how these human-induced changes interact. In particular, ZM may impact biogeochemical transformations. We hypothesized that high NH4+ waste from ZM will increase sediment nitrification rates, and increase NO3--N available for denitrification. To test this prediction, aquaria were stocked with either no ZM or 10,000 individuals m-2. Sediment nitrification (via nitrapyrin-inhibition technique), denitrification (via chloramphenicol-amended acetylene block technique), and sediment O2 profiles (using microelectrodes) were measured weekly for 1 month. The presence of ZM increased nitrification and denitrification rates over initial conditions throughout all weeks (RM ANOVA, p<0.05). Sediment nitrification in the ZM aquaria increased to 47 ± 2.1 μ g NH4+ -NgAFDM-1h-1 by the 3rd week in comparison to 24 ± 4.4 μ g NH4+ -NgAFDM-1h-1 in control aquaria. Sediment denitrification peaked at week 2 in the ZM aquaria at 330 ± 24 μ g N2O gAFDM-1h-1 but remained low in control aquaria throughout. There was a stronger correlation between nitrification and denitrification rates in the presence of ZM (R=0.32, 0.12 respectively). These results, in addition to more frequent hypoxia in sediment oxygen profiles, suggest closer coupling between nitrification and denitrification when ZM are present.

  5. High frequency homogenization for structural mechanics

    NASA Astrophysics Data System (ADS)

    Nolde, E.; Craster, R. V.; Kaplunov, J.

    2011-03-01

    We consider a net created from elastic strings as a model structure to investigate the propagation of waves through semi-discrete media. We are particularly interested in the development of continuum models, valid at high frequencies, when the wavelength and each cell of the net are of similar order. Net structures are chosen as these form a general two-dimensional example, encapsulating the essential physics involved in the two-dimensional excitation of a lattice structure whilst retaining the simplicity of dealing with elastic strings. Homogenization techniques are developed here for wavelengths commensurate with the cellular scale. Unlike previous theories, these techniques are not limited to low frequency or static regimes, and lead to effective continuum equations valid on a macroscale with the details of the cellular structure encapsulated only through integrated quantities. The asymptotic procedure is based upon a two-scale approach and the physical observation that there are frequencies that give standing waves, periodic with the period or double-period of the cell. A specific example of a net created by a lattice of elastic strings is constructed, the theory is general and not reliant upon the net being infinite, none the less the infinite net is a useful special case for which Bloch theory can be applied. This special case is explored in detail allowing for verification of the theory, and highlights the importance of degenerate cases; the specific example of a square net is treated in detail. An additional illustration of the versatility of the method is the response to point forcing which provides a stringent test of the homogenized equations; an exact Green's function for the net is deduced and compared to the asymptotics.

  6. Performance of annular high frequency thermoacoustic engines

    NASA Astrophysics Data System (ADS)

    Rodriguez, Ivan A.

    This thesis presents studies of the behavior of miniature annular thermoacoustic prime movers and the imaging of the complex sound fields using PIV inside the small acoustic wave guides when driven by a temperature gradient. Thermoacoustic engines operating in the standing wave mode are limited in their acoustic efficiency by a high degree of irreversibility that is inherent in how they work. Better performance can be achieved by using traveling waves in the thermoacoustic devices. This has led to the development of an annular high frequency thermoacoustic prime mover consisting of a regenerator, which is a random stack in-between a hot and cold heat exchanger, inside an annular waveguide. Miniature devices were developed and studied with operating frequencies in the range of 2-4 kHz. This corresponds to an average ring circumference of 11 cm for the 3 kHz device, the resonator bore being 6 mm. A similar device of 11 mm bore, length of 18 cm was also investigated; its resonant frequency was 2 kHz. Sound intensities as high as 166.8 dB were generated with limited heat input. Sound power was extracted from the annular structure by an impedance-matching side arm. The nature of the acoustic wave generated by heat was investigated using a high speed PIV instrument. Although the acoustic device appears symmetric, its performance is characterized by a broken symmetry and by perturbations that exist in its structure. Effects of these are observed in the PIV imaging; images show axial and radial components. Moreover, PIV studies show effects of streaming and instabilities which affect the devices' acoustic efficiency. The acoustic efficiency is high, being of 40% of Carnot. This type of device shows much promise as a high efficiency energy converter; it can be reduced in size for microcircuit applications.

  7. High frequency of tumours in Mulibrey nanism.

    PubMed

    Karlberg, Niklas; Karlberg, Susann; Karikoski, Riitta; Mikkola, Sakari; Lipsanen-Nyman, Marita; Jalanko, Hannu

    2009-06-01

    Mulibrey nanism (MUL) is a monogenic disorder with prenatal-onset growth failure, typical clinical characteristics, cardiopathy and tendency for a metabolic syndrome. It is caused by recessive mutations in the TRIM37 gene encoding for the peroxisomal TRIM37 protein with ubiquitin-ligase activity. In this work, the frequency and pathology of malignant and benign tumours were analysed in a national cohort of 89 Finnish MUL patients aged 0.7-76 years. The subjects had a clinical and radiological evaluation, and histological and immunohistocemical analyses on specimens obtained from biopsy, surgery or autopsy, were performed. The results show that the MUL patients have disturbed architecture with ectopic tissues and a high frequency of both benign and malignant tumours detectable in several internal organs. A total of 210 tumorous lesions were detected in 66/89 patients (74%). Fifteen malignancies occurred in 13 patients (15%), seven of them in the kidney (five Wilms' tumours), three in the thyroid gland, two gynaecological cancers, one gastrointestinal carcinoid tumour, one neuropituitary Langerhans cell histiocytosis and one case of acute lymphoblastic leukaemia (ALL). Tumours detected by radiology in the liver and other organs mainly comprised strongly dilated blood vessels (peliosis), vascularized cysts and nodular lesions. The lesions showed strong expression of the endothelial cell markers CD34 and CD31 as well as the myocyte marker alpha-smooth muscle actin (alpha-SMA). Our findings show that MUL is associated with frequent malignant tumours and benign adenomatous and vascular lesions, as well as disturbed organ development. PMID:19334051

  8. High frequency variability of particle size distribution and its dependency on turbulence over the sea bottom during re-suspension processes

    NASA Astrophysics Data System (ADS)

    Renosh, P. R.; Schmitt, François G.; Loisel, Hubert; Sentchev, Alexei; Mériaux, Xavier

    2014-04-01

    The impact of tidal current, waves and turbulence on particles re-suspension over the sea bottom is studied through Eulerian high frequency measurements of velocity and particle size distribution (PSD) during 5 tidal cycles (65 h) in a coastal environment of the eastern English Channel. High frequency variability of PSD is observed along with the velocity fluctuations. Power spectral analysis shows that turbulent velocity and PSD parameters have similarities in their spectral behaviour over the whole range of examined temporal scales. The low frequency variability of particles is controlled by turbulence (β≃-5/3) and the high frequency is partly driven by dynamical processes impacted by the sea bottom interactions with turbulence (wall turbulence). Stokes number (St), rarely measured in situ, exhibits very low values, emphasizing that these particles can be considered as passive tracers. The effect of tide and waves on turbidity and PSD is highlighted. During slack tide, when the current reaches its minimum value, we observe a higher proportion of small particles compared to larger ones. To a lower extent, high significant wave heights are also associated with a greater concentration of suspended sediments and the presence of larger particles (larger Sauter's diameter DA, and lower PSD slope ξ).

  9. Biotic and abiotic pathways of phosphorus cycling in minerals and sediments: insights from oxygen isotopes in phosphate

    SciTech Connect

    Jaisi, Deb P.; Kukkadapu, Ravi K.; Stout, Lisa M.; Varga, Tamas; Blake, Ruth E.

    2011-07-06

    A key question to address in the development of oxygen isotope ratios in phosphate (18Op) as a tracer of biogeochemical cycling of phosphorus in ancient and modern environments is the nature of isotopic signatures associated with uptake and cycling of mineral-bound phosphate by microorganisms. Here we present experimental results aimed at understanding the biotic and abiotic pathway of P cycling during biological uptake of phosphate sorbed to ferrihydrite and the selective uptake of specific sedimentary phosphate phases by Escherichia coli, Vibrio fischeri and Marinobacter aquaeolei. Results indicate that a significant fraction of ferrihydrite-bound phosphate is biologically available. The fraction of phosphate taken up by E. coli attained an equilibrium isotopic composition in a short time (<50 hrs) due to efficient O-isotope exchange between phosphate and water (biotic pathway). The difference in isotopic composition between newly equilibrated aqueous and residual sorbed phosphate promoted the exchange of intact phosphate radicals (abiotic pathway) so that this difference gradually became negligible. In sediment containing different P phases, E. coli and V. fischeri ‘extracted’ loosely sorbed phosphate first while M. aquaeolei preferred iron-oxide bound phosphate. Each bacterium imprinted a biotic isotopic signature on each P phase that it took up and cycled. For example, the 18Op value of the sorbed phosphate phase shifted gradually towards equilibrium isotopic composition and the value of Fe oxide-bound phosphate showed slight changes at first, but when new iron oxides were formed, co-precipitated/occluded phosphate retained 18O values of aqueous phosphate at that time. Concentrations and isotopic compositions of authigenic and detrital phosphates did not change, suggesting that these phosphate phases were not utilized by bacteria. These findings support burgeoning applications of 18Op as a tracer of phosphorus cycling in sediments, soils and aquatic

  10. Three cycles of sedimentation in ancient sedimentary basins of southern Ireland: insights from detrital zircon U-Pb ages

    NASA Astrophysics Data System (ADS)

    Fairey, Brenton; Kerrison, Aidan; Meere, Patrick; Mulchrone, Kieran; Linnemann, Ulf; Hofmann, Mandy; Gaertner, Andreas; Sonntag, Benita-Lisette; Byrne, Keith

    2016-04-01

    Previous work has shown that sedimentary rocks from the Lower Devonian Dingle Basin were uplifted and recycled by Acadian orogenic activity into the Upper Devonian Munster Basin. This is particularly applicable for sediments deposited in the western part of the Munster Basin. In the present study, a new dataset of U-Pb ages for detrital zircons has been established that spans a large geographic area which includes the Dingle and Munster basins as well as the offshore Mesozoic North Celtic Sea, South Celtic Sea, 'Goban Spur' and Fastnet basins. The study is the first of its kind in any of these sedimentary basins. The aim is to investigate whether sediments deposited in the offshore basins during the Mesozoic reflect three erosion-deposition cycles. Detritus that has undergone three sedimentary cycles would yield super-mature sediments suitable for hydrocarbon storage. Detrital zircon age spectra for Lower Devonian Dingle Basin samples indicate strong sediment input from Avalonian (~600 Ma) and Laurentian (~1.7 Ga and ~1.1 Ga) sources with some input from Caledonian orogenic sources (400-480 Ma). Detrital zircon age spectra in the western Munster Basin largely reflect input from Caledonian-aged igneous crustal input (400-480 Ma) and Laurentian sources. An Avalonian component is not detected in any of the samples from the western Munster Basin. In the central and eastern parts of the Munster Basin, detrital zircon age spectra indicate that the dominant sources of detritus are derived from Laurentia and from Caledonian igneous rocks. In contrast to the western part of the basin, age components around 600 Ma are present in some samples and represent an Avalonian source. These signals are echoed, at varying degrees, in detrital age spectra from Jurassic and Cretaceous samples of the central North Celtic Sea Basin. These age spectra also indicate a significant contribution of detritus from Avalonian terrane. The Avalonian signature is completely absent from Jurassic

  11. Deposit-Feeding Sea Cucumbers Enhance Mineralization and Nutrient Cycling in Organically-Enriched Coastal Sediments

    PubMed Central

    MacTavish, Thomas; Stenton-Dozey, Jeanie; Vopel, Kay; Savage, Candida

    2012-01-01

    Background Bioturbators affect multiple biogeochemical interactions and have been suggested as suitable candidates to mitigate organic matter loading in marine sediments. However, predicting the effects of bioturbators at an ecosystem level can be difficult due to their complex positive and negative interactions with the microbial community. Methodology/Principal Findings We quantified the effects of deposit-feeding sea cucumbers on benthic algal biomass (microphytobenthos, MPB), bacterial abundance, and the sediment–seawater exchange of dissolved oxygen and nutrients. The sea cucumbers increased the efflux of inorganic nitrogen (ammonium, NH4+) from organically enriched sediments, which stimulated algal productivity. Grazing by the sea cucumbers on MPB (evidenced by pheopigments), however, caused a net negative effect on primary producer biomass and total oxygen production. Further, there was an increased abundance of bacteria in sediment with sea cucumbers, suggesting facilitation. The sea cucumbers increased the ratio of oxygen consumption to production in surface sediment by shifting the microbial balance from producers to decomposers. This shift explains the increased efflux of inorganic nitrogen and concordant reduction in organic matter content in sediment with bioturbators. Conclusions/Significance Our study demonstrates the functional role and potential of sea cucumbers to ameliorate some of the adverse effects of organic matter enrichment in coastal ecosystems. PMID:23209636

  12. High Frequency QRS ECG Accurately Detects Cardiomyopathy

    NASA Technical Reports Server (NTRS)

    Schlegel, Todd T.; Arenare, Brian; Poulin, Gregory; Moser, Daniel R.; Delgado, Reynolds

    2005-01-01

    High frequency (HF, 150-250 Hz) analysis over the entire QRS interval of the ECG is more sensitive than conventional ECG for detecting myocardial ischemia. However, the accuracy of HF QRS ECG for detecting cardiomyopathy is unknown. We obtained simultaneous resting conventional and HF QRS 12-lead ECGs in 66 patients with cardiomyopathy (EF = 23.2 plus or minus 6.l%, mean plus or minus SD) and in 66 age- and gender-matched healthy controls using PC-based ECG software recently developed at NASA. The single most accurate ECG parameter for detecting cardiomyopathy was an HF QRS morphological score that takes into consideration the total number and severity of reduced amplitude zones (RAZs) present plus the clustering of RAZs together in contiguous leads. This RAZ score had an area under the receiver operator curve (ROC) of 0.91, and was 88% sensitive, 82% specific and 85% accurate for identifying cardiomyopathy at optimum score cut-off of 140 points. Although conventional ECG parameters such as the QRS and QTc intervals were also significantly longer in patients than controls (P less than 0.001, BBBs excluded), these conventional parameters were less accurate (area under the ROC = 0.77 and 0.77, respectively) than HF QRS morphological parameters for identifying underlying cardiomyopathy. The total amplitude of the HF QRS complexes, as measured by summed root mean square voltages (RMSVs), also differed between patients and controls (33.8 plus or minus 11.5 vs. 41.5 plus or minus 13.6 mV, respectively, P less than 0.003), but this parameter was even less accurate in distinguishing the two groups (area under ROC = 0.67) than the HF QRS morphologic and conventional ECG parameters. Diagnostic accuracy was optimal (86%) when the RAZ score from the HF QRS ECG and the QTc interval from the conventional ECG were used simultaneously with cut-offs of greater than or equal to 40 points and greater than or equal to 445 ms, respectively. In conclusion 12-lead HF QRS ECG employing

  13. The high frequency fatigue behavior of continuous-fiber-reinforced ceramic matrix composites

    NASA Astrophysics Data System (ADS)

    Chawla, Nikhilesh

    Many potential applications for continuous fiber ceramic matrix composites (CFCMCs), such as gas turbines and heat exchangers, will involve high frequency cyclic loading (75 Hz or higher). While most of the work in the area of fatigue of CFCMCs has concentrated on low frequency behavior, it has been shown that fatigue at high frequencies can exacerbate the accumulation of microstructural damage and significantly decrease fatigue life. "Soft" matrix composites with strong interface bonding provided superior resistance to high frequency fatigue damage. Nicalon/SiCON composites with strong interfacial bonding between the fibers and matrix exhibited very little internal heating during high frequency fatigue loading. This composite system exhibited excellent fatigue life, with fatigue runout at 10sp7 cycles occurring for stresses close to 80% of the ultimate strength (at a loading frequency of 100 Hz). Thick fiber coatings may be more effective in reducing the amount of fiber wear and damage which occur during high frequency fatigue. More effective lubrication at the fiber/matrix interface was achieved with thicker carbon coatings in Nicalon/C/SiC composites subjected to high frequency fatigue loading. Composites with thicker coatings exhibited substantially lower frictional heating and had much higher fatigue lives. The effect of laminate stacking sequence had a significant effect on the high frequency fatigue behavior of CFCMCs. In SCS-6/Sisb3Nsb4 composites, frictional heating in angle-ply laminates (±45) was substantially higher than that in cross-ply laminates (0/90). Since the angle-ply had a lower stiffness, matrix microcracking in this composite was more predominant. Finally, preliminary fatigue damage mechanism maps for CFCMCs were developed. These maps provided a means to identify which fatigue mechanisms were operating at a given stress level and number of cycles.

  14. High power, high frequency helix TWT's

    NASA Astrophysics Data System (ADS)

    Sloley, H. J.; Willard, J.; Paatz, S. R.; Keat, M. J.

    The design and performance characteristics of a 34-GHz pulse tube capable of 75 W peak power output at 30 percent duty cycle and a broadband CW tube are presented. Particular attention is given to the engineering problems encountered during the development of the tubes, including the suppression of backward wave oscillation, the design of electron guns for small-diameter high-current beams, and the thermal capability of small helix structures. The discussion also covers the effects of various design parameters and choice of engineering materials on the ultimate practical limit of power and gain at the operating frequencies. Measurements are presented for advanced experimental tubes.

  15. Development of a Complete Life Cycle Sediment Toxicity Test for the Sheepshead Minnow (Cyprinodon variegatus)

    EPA Science Inventory

    Existing sediment toxicity test methods are limited to acute and chronic exposure of invertebrates and acute exposure of vertebrates, with limited guidance on the chronic exposure of vertebrates, specifically fishes. A series of life stage-specific studies were conducted to dete...

  16. Numerical modelling of subglacial erosion and sediment transport and its application to the North American ice sheets over the Last Glacial cycle

    NASA Astrophysics Data System (ADS)

    Melanson, Alexandre; Bell, Trevor; Tarasov, Lev

    2013-05-01

    Present-day sediment distribution offers a potentially strong constraint on past ice sheet evolution. Glacial system models (GSMs), however, cannot address this constraint while lacking appropriate representations of subglacial sediment production and transport. Incorporating these elements in GSMs is also required in order to quantify the impact of a changing sediment cover on glacial cycle dynamics. Towards these goals, we present a subglacial process model (hereafter referred to as the sediment model) that incorporates mechanisms for sediment production, entrainment, transport, and deposition. Bedrock erosion is calculated by both Hallet's and Boulton's abrasion laws separately, and by a novel quarrying law parametrized as a function of subglacial cavity extent. These process-oriented erosion laws are compared against a simple empirical relationship between erosion rate and the work done by basal stress. Sediment entrainment is represented by Philip's law for regelation intrusion and soft-bed deformation is included as a subglacial sediment transport mechanism. The model is driven by the data-calibrated MUN (3D) GSM and a newly developed subglacial hydrology module. The sediment model is applied to the last North American glacial cycle and predicts sediment thickness and cumulative erosion patterns. Results are obtained in the context of a sensitivity analysis and are compared against the present-day distribution of glacigenic sediment and geological estimates of Laurentide Ice Sheet erosion. Given plausible ranges for the sensitivity parameters, chosen a priori based on available literature or on heuristic arguments, the calculated erosion depths overlap with the geological estimates of Laurentide erosion. Most of the runs in the sensitivity set produce unrealistically thick and continuous moraines along the eastern, southern and western margins of the North American ice complex, which suggests that the model overestimates sediment entrainment and thus

  17. Microbial Nitrogen Cycling Associated with the Early Diagenesis of Organic Matter in Subseafloor Sediments

    NASA Astrophysics Data System (ADS)

    Zhao, R.

    2015-12-01

    The early diagenesis of organic matter is the major energy source of marine sedimentary biosphere and thus controls its population size; however, the vertical distribution of any functional groups along with the diagenesis of organic matter is remained unclear, especially for those microbes involved in nitrogen transformation which serve as a major control on the nitrogen flux between reservoirs. Here we investigated the vertical distributions of various functional groups in five sediment cores retrieved from Arctic Mid-Ocean Ridge (AMOR), with emphasis on the nitrifiers, denitrifiers and anaerobic ammonium oxidizing bacteria (anammox). We observed the clear geochemical zonation associated with organic matter diagenesis in the sediments based on the pore water profiles of oxygen, nitrate, ammonium, manganese and sulfate, with distinct geochemical transition zones at the boundaries of geochemical zones, including oxic-anoxic transition zone (OATZ) and nitrate-manganese reduction zone (NMTZ). Nitrate was produced in surface oxygenated sediments and nitrate consumption mainly took place at the NMTZ, splitted between re-oxidation of ammonium and manganese (II). Abundances of ammonia oxidizers, nitrite oxidizers, and denitrifiers, estimated through quantitative PCR targeting their respective functional genes, generally decrease with depth, but constantly elevated around the OATZ, NMTZ, and manganese-reduction zone as well. Anammox bacteria were only detected around the NMTZ where both nitrate/nitrite and ammonium are available. These depth profiles of functional groups were also confirmed by the community structure profiling by prokaryotic 16S rRNA gene tag pyrosequencing. Cell-specific rates of nitrification and denitrification, calculated from the bulk net reaction rates divided by functional group abundances, were similar to those values from oligotrophic sediments like North Pond and thus suggested that nitrifiers and denitirifiers populations were in maintenance

  18. Carbon, nutrient and trace metal cycling in sandy sediments: A comparison of high-energy beaches and backbarrier tidal flats

    NASA Astrophysics Data System (ADS)

    Reckhardt, Anja; Beck, Melanie; Seidel, Michael; Riedel, Thomas; Wehrmann, Achim; Bartholomä, Alexander; Schnetger, Bernhard; Dittmar, Thorsten; Brumsack, Hans-Jürgen

    2015-06-01

    In order to evaluate the importance of coastal sandy sediments and their contribution to carbon, nutrient and metal cycling we investigated two beach sites on Spiekeroog Island, southern North Sea, Germany, and a tidal flat margin, located in Spiekeroog's backbarrier area. We also analyzed seawater and fresh groundwater on Spiekeroog Island, to better define endmember concentrations, which influence our study sites. Intertidal sandy flats and beaches are characterized by pore water advection. Seawater enters the sediment during flood and pore water drains out during ebb and at low tide. This pore water circulation leads to continuous supply of fresh organic substrate to the sediments. Remineralization products of microbial degradation processes, i.e. nutrients, and dissolved trace metals from the reduction of particulate metal oxides, are enriched in the pore water compared to open seawater concentrations. The spatial distribution of dissolved organic carbon (DOC), nutrients (PO43-, NO3-, NO2-, NH4+, Si(OH)4 and total alkalinity), trace metals (dissolved Fe and Mn) as well as sulfate suggests that the exposed beach sites are subject to relatively fast pore water advection, which leads to organic matter and oxygen replenishment. Frequent pore water exchange further leads to comparatively low nutrient concentrations. Sulfate reduction does not appear to play a major role during organic matter degradation. High nitrate concentrations indicate that redox conditions are oxic within the duneward freshwater influenced section, while ammonification, denitrification, manganese and iron reduction seem to prevail in the ammonium-dominated seawater circulation zone. In contrast, the sheltered tidal flat margin site exhibits a different sedimentology (coarser beach sands versus finer tidal flat sands) and nutrients, dissolved manganese and DOC accumulate in the pore water. Ammonium is the dominant pore water nitrogen species and intense sulfate reduction leads to the formation

  19. Life of fluorescent lamps operated at high frequencies with solid-state ballasts

    NASA Astrophysics Data System (ADS)

    Verderber, R. R.; Morse, O.; Rubinstein, F. M.

    1985-07-01

    Standard 40-watt, F-40, rapid-start, fluorescent lamps were operated with solid-state ballasts following the standard life-testing cycle of 3 hours on and 20 minutes off for more than 20,000 hours at high frequency. Lamp operating characteristics (starting voltage, filament voltage, arc current, and current-crest factor) were studied as factors affecting lamp life. Measurements show that fluorescent lamps can attain rated life at high frequency using solid-state ballasts. When lamps are operated in the dimmed mode, full filament power is required to sustain lamplife. The rate of lamp lumen depreciation is dependent on the lamp loading and not the operating frequency.

  20. S and O Isotope Studies of Microbial S Cycling in the Deep Biosphere of Marine Sediments: Eastern Equatorial Pacific Ocean

    NASA Astrophysics Data System (ADS)

    Blake, R. E.; Bottcher, M. E.; Surkov, A. V.; Ferdelman, T. G.; Jorgensen, B. B.

    2004-12-01

    We have determined the oxygen (18O/16O) and sulfur (34S/32S) isotope ratios of porewater sulfate to depths of over 400 mbsf in sediments from open-ocean and upwelling sites in the Eastern Equatorial Pacific ocean. Sulfate δ 18O ranges from near-normal seawater values (9.5 permil) at organic-poor open-ocean sites, to approximately 30 permil at sites with higher organic matter content and higher associated microbial activity. Depth-correlative trends of δ 18O, δ 34S, alkalinity, methane, ammonium and the presence of sulfide, indicate significant oxidation of sedimentary organic matter by sulfate-reducing microbial populations as well as anaerobic oxidation of methane. δ 18O-SO4 values at low-activity sites reveal the presence of significant microbial sulfur-cycling activity despite relatively flat sulfate concentration and δ 34S profiles. This activity may include contributions from several processes including: enzyme-catalyzed equilibration between oxygen in sulfate and water superimposed upon microbial sulfate reduction, sulfide oxidation, and bacterial disproportionation of sulfur intermediates. Large isotope enrichment factors observed at low-activity sites (40-80 permil) likely reflect concurrent processes of: kinetic isotope fractionation, equilibrium fractionation between sulfate and water, and sulfide oxidation at low rates of sulfate reduction. Results of this study indicate that coupled measurements of S and O isotope ratios of porewater sulfate are a powerful tool for tracing microbial activity and sulfur cycling in marine sediments.

  1. Bacterial communities potentially involved in iron-cycling in Baltic Sea and North Sea sediments revealed by pyrosequencing.

    PubMed

    Reyes, Carolina; Dellwig, Olaf; Dähnke, Kirstin; Gehre, Matthias; Noriega-Ortega, Beatriz E; Böttcher, Michael E; Meister, Patrick; Friedrich, Michael W

    2016-04-01

    To gain insight into the bacterial communities involved in iron-(Fe) cycling under marine conditions, we analysed sediments with Fe-contents (0.5-1.5 wt %) from the suboxic zone at a marine site in the Skagerrak (SK) and a brackish site in the Bothnian Bay (BB) using 16S rRNA gene pyrosequencing. Several bacterial families, including Desulfobulbaceae, Desulfuromonadaceae and Pelobacteraceae and genera, includingDesulfobacterandGeobacter, known to reduce Fe were detected and showed highest abundance near the Fe(III)/Fe(II) redox boundary. Additional genera with microorganisms capable of coupling fermentation to Fe-reduction, includingClostridiumandBacillus, were observed. Also, the Fe-oxidizing families Mariprofundaceae and Gallionellaceae occurred at the SK and BB sites, respectively, supporting Fe-cycling. In contrast, the sulphate (SO4 (2-)) reducing bacteriaDesulfococcusandDesulfobacteriumwere more abundant at greater depths concurring with a decrease in Fe-reducing activity. The communities revealed by pyrosequencing, thus, match the redox stratification indicated by the geochemistry, with the known Fe-reducers coinciding with the zone of Fe-reduction. Not the intensely studied model organisms, such asGeobacterspp., but rather versatile microorganisms, including sulphate reducers and possibly unknown groups appear to be important for Fe-reduction in these marine suboxic sediments. PMID:26960392

  2. On-clip high frequency reliability and failure test structures

    DOEpatents

    Snyder, E.S.; Campbell, D.V.

    1997-04-29

    Self-stressing test structures for realistic high frequency reliability characterizations. An on-chip high frequency oscillator, controlled by DC signals from off-chip, provides a range of high frequency pulses to test structures. The test structures provide information with regard to a variety of reliability failure mechanisms, including hot-carriers, electromigration, and oxide breakdown. The system is normally integrated at the wafer level to predict the failure mechanisms of the production integrated circuits on the same wafer. 22 figs.

  3. On-clip high frequency reliability and failure test structures

    DOEpatents

    Snyder, Eric S.; Campbell, David V.

    1997-01-01

    Self-stressing test structures for realistic high frequency reliability characterizations. An on-chip high frequency oscillator, controlled by DC signals from off-chip, provides a range of high frequency pulses to test structures. The test structures provide information with regard to a variety of reliability failure mechanisms, including hot-carriers, electromigration, and oxide breakdown. The system is normally integrated at the wafer level to predict the failure mechanisms of the production integrated circuits on the same wafer.

  4. Reversible tobramycin-induced bilateral high-frequency vestibular toxicity.

    PubMed

    Walsh, R M; Bath, A P; Bance, M L

    2000-01-01

    We report an unusual case of tobramycin-induced bilateral high-frequency vestibular toxicity with subsequent clinical and objective evidence of functional recovery. In those patients with a clinical presentation suggestive of aminoglycoside-induced bilateral vestibular toxicity (ataxia and oscillopsia) and normal low-frequency (ENG-caloric) responses, high-frequency rotation chair testing should be performed to exclude a high-frequency vestibular deficit. PMID:10810261

  5. High-frequency Probing Diagnostic for Hall Current Plasma Thrusters

    SciTech Connect

    A.A. Litvak; Y. Raitses; N.J. Fisch

    2001-10-25

    High-frequency oscillations (1-100 MHz) in Hall thrusters have apparently eluded significant experimental scrutiny. A diagnostic setup, consisting of a single Langmuir probe, a special shielded probe connector-positioner, and an electronic impedance-matching circuit, was successfully built and calibrated. Through simultaneous high-frequency probing of the Hall thruster plasma at multiple locations, high-frequency plasma waves have been identified and characterized for various thruster operating conditions.

  6. Sensor Measurements and Sediment Incubations Indicate Diurnal Redox Cycling Associate With Arsenic Mobilization at a Bangladeshi Rice Paddy

    NASA Astrophysics Data System (ADS)

    Lin, T.; Lin, C.; Ramanathan, N.; Neumann, R.; Harvey, C.; Jay, J.

    2007-12-01

    The presence of arsenic in the groundwater has led to the largest environmental poisoning in history; tens of millions of people in the Ganges Delta continue to drink groundwater that is dangerously contaminated with arsenic (As). Rice fields receive large loads of arsenic with irrigation water and provide recharge to the underlying aquifer. It is currently not known whether rice fields are a sink or source of arsenic in the hydrologic system. In the dry season, as As(III)-containing minerals are oxidized, As(V) is released and will adhere to Fe hydr(oxide) minerals. When sediments are inundated with water, reducing conditions will then drive reduction of Fe hydr(oxides) and release of As. We have been intensively studying a field site in Munshiganj, Bangladesh with extremely high levels of arsenic in groundwater (up to 1.2 mg/L). To better understand geochemical and microbial processes leading to As mobilization in surface sediment, we deployed sensors to take temporally dense measurements across our experimental rice paddy. Data collected in both 2006 and 2007 showed trends in geochemical parameters indicating that diurnal, possibly plant-induced, processes may be important. Over a two month period, nitrate concentrations decrease consistently each day as ammonium levels increase, presumably through temperature driven reductive processes. Nitrate concentrations in the subsurface then increase while ammonium levels decrease, possibly due to root oxygen leakage or rapid infiltration of oxygen rich surface water. Using sediment from the rice paddy and artificial irrigation water, laboratory microcosms were constructed to simulate the diurnal cycles observed at the field site. In carbon-ammended treatments, Fe and As cycling can occur on the order of days. Oscillations in redox conditions on diurnal as well as seasonal time scales may be important in the mobilization of arsenic into aquifers. By elucidating As mobilization mechanisms at an experimental rice paddy

  7. Effects of sediment dredging on nitrogen cycling in Lake Taihu, China: Insight from mass balance based on a 2-year field study.

    PubMed

    Yu, Juhua; Fan, Chengxin; Zhong, Jicheng; Zhang, Lu; Zhang, Lei; Wang, Changhui; Yao, Xiaolong

    2016-02-01

    Sediment dredging can permanently remove pollutants from an aquatic ecosystem, which is considered an effective approach to aquatic ecosystem restoration. In this work, a 2-year field simulation test was carried out to investigate the effect of dredging on nitrogen cycling across the sediment-water interface (SWI) in Lake Taihu, China. The results showed that simulated dredging applied to an area rich in total organic carbon (TOC) and total nitrogen (TN) slightly reduced the NH4(+)-N release from sediments while temporarily enhanced the NH4(+)-N release in an area with lower TOC and/or TN (in the first 180 days), although the application had a limited effect on the fluxes of NO2(-)-N and NO3(-)-N in both areas. Further analysis indicated that dredging induced decreases in nitrification, denitrification, and anaerobic ammonium oxidation (anammox) in sediments, notably by 76.9, 49.0, and 89.9%, respectively, in the TOC and/or TN-rich area. Therefore, dredging slowed down nitrogen cycling rates in sediments but did not increase N loading to overlying water. The main reason for the above phenomenon could be attributed to the removal of the surface sediments enriched with more TOC and/or TN (compared with the bottom sediments). Overall, to minimize internal N pollution, dredging may be more applicable to nutrient-rich sediments. PMID:26499196

  8. Anaerobic oxidation of short-chain alkanes in hydrothermal sediments: potential influences on sulfur cycling and microbial diversity

    PubMed Central

    Adams, Melissa M.; Hoarfrost, Adrienne L.; Bose, Arpita; Joye, Samantha B.; Girguis, Peter R.

    2013-01-01

    Short-chain alkanes play a substantial role in carbon and sulfur cycling at hydrocarbon-rich environments globally, yet few studies have examined the metabolism of ethane (C2), propane (C3), and butane (C4) in anoxic sediments in contrast to methane (C1). In hydrothermal vent systems, short-chain alkanes are formed over relatively short geological time scales via thermogenic processes and often exist at high concentrations. The sediment-covered hydrothermal vent systems at Middle Valley (MV, Juan de Fuca Ridge) are an ideal site for investigating the anaerobic oxidation of C1–C4 alkanes, given the elevated temperatures and dissolved hydrocarbon species characteristic of these metalliferous sediments. We examined whether MV microbial communities oxidized C1–C4 alkanes under mesophilic to thermophilic sulfate-reducing conditions. Here we present data from discrete temperature (25, 55, and 75°C) anaerobic batch reactor incubations of MV sediments supplemented with individual alkanes. Co-registered alkane consumption and sulfate reduction (SR) measurements provide clear evidence for C1–C4 alkane oxidation linked to SR over time and across temperatures. In these anaerobic batch reactor sediments, 16S ribosomal RNA pyrosequencing revealed that Deltaproteobacteria, particularly a novel sulfate-reducing lineage, were the likely phylotypes mediating the oxidation of C2–C4 alkanes. Maximum C1–C4 alkane oxidation rates occurred at 55°C, which reflects the mid-core sediment temperature profile and corroborates previous studies of rate maxima for the anaerobic oxidation of methane (AOM). Of the alkanes investigated, C3 was oxidized at the highest rate over time, then C4, C2, and C1, respectively. The implications of these results are discussed with respect to the potential competition between the anaerobic oxidation of C2–C4alkanes with AOM for available oxidants and the influence on the fate of C1 derived from these hydrothermal systems. PMID:23717305

  9. On the Importance of High Frequency Gravity Waves for Ice Nucleation in the Tropical Tropopause Layer

    NASA Technical Reports Server (NTRS)

    Jensen, Eric J.

    2016-01-01

    Recent investigations of the influence of atmospheric waves on ice nucleation in cirrus have identified a number of key processes and sensitivities: (1) ice concentrations produced by homogeneous freezing are strongly dependent on cooling rates, with gravity waves dominating upper tropospheric cooling rates; (2) rapid cooling driven by high-frequency waves are likely responsible for the rare occurrences of very high ice concentrations in cirrus; (3) sedimentation and entrainment tend to decrease ice concentrations as cirrus age; and (4) in some situations, changes in temperature tendency driven by high-frequency waves can quench ice nucleation events and limit ice concentrations. Here we use parcel-model simulations of ice nucleation driven by long-duration, constant-pressure balloon temperature time series, along with an extensive dataset of cold cirrus microphysical properties from the recent ATTREX high-altitude aircraft campaign, to statistically examine the importance of high-frequency waves as well as the consistency between our theoretical understanding of ice nucleation and observed ice concentrations. The parcel-model simulations indicate common occurrence of peak ice concentrations exceeding several hundred per liter. Sedimentation and entrainment would reduce ice concentrations as clouds age, but 1-D simulations using a wave parameterization (which underestimates rapid cooling events) still produce ice concentrations higher than indicated by observations. We find that quenching of nucleation events by high-frequency waves occurs infrequently and does not prevent occurrences of large ice concentrations in parcel simulations of homogeneous freezing. In fact, the high-frequency variability in the balloon temperature data is entirely responsible for production of these high ice concentrations in the simulations.

  10. Trace element cycling in a subterranean estuary: Part 1. Geochemistry of the permeable sediments

    NASA Astrophysics Data System (ADS)

    Charette, Matthew A.; Sholkovitz, Edward R.; Hansel, Colleen M.

    2005-04-01

    Subterranean estuaries are characterized by the mixing of terrestrially derived groundwater and seawater in a coastal aquifer. Subterranean estuaries, like their river water-seawater counterparts on the surface of the earth, represent a major, but less visible, hydrological and geochemical interface between the continents and the ocean. This article is the first in a two-part series on the biogeochemistry of the subterranean estuary at the head of Waquoit Bay (Cape Cod, MA, USA). The pore-water distributions of salinity, Fe and Mn establish the salt and redox framework of this subterranean estuary. The biogeochemistry of Fe, Mn, P, Ba, U and Th will be addressed from the perspective of the sediment composition. A second article will focus on the groundwater and pore-water chemistries of Fe, Mn, U and Ba. Three sediment cores were collected from the head of Waquoit Bay where the coastal aquifer consists of permeable sandy sediment. A selective dissolution method was used to measure the concentrations of P, Ba, U and Th that are associated with "amorphous (hydr)oxides of iron and manganese" and "crystalline Fe and Mn (hydr)oxides." The deeper sections of the cores are characterized by large amounts of iron (hydr)oxides that are precipitated onto organic C-poor quartz sand from high-salinity pore waters rich in dissolved ferrous iron. Unlike Fe (hydr)oxides, which increase with depth, the Mn (hydr)oxides display midcore maxima. This type of vertical stratification is consistent with redox-controlled diagenesis in which Mn (hydr)oxides are formed at shallower depths than iron (hydr)oxides. P and Th are enriched in the deep sections of the cores, consistent with their well-documented affinity for Fe (hydr)oxides. In contrast, the downcore distribution of Ba, especially in core 3, more closely tracks the concentration of Mn (hydr)oxides. Even though Mn (hydr)oxides are 200-300 times less abundant than Fe (hydr)oxides in the cores, Mn (hydr)oxides are known to have an

  11. Cycling of trace metals (Mn, Fe, Mo, U, V, Cr) in deep pore waters of intertidal flat sediments

    NASA Astrophysics Data System (ADS)

    Beck, Melanie; Dellwig, Olaf; Schnetger, Bernhard; Brumsack, Hans-Jürgen

    2008-06-01

    Trace metals (Mn, Fe, Mo, U, Cr, V) were studied in pore waters of an intertidal flat located in the German Wadden Sea. The study system is an example of a permeable tidal flat system where pore water exchange is affected by tidal driven pressure gradients besides diffusion. Permanently installed in situ samplers were used to extract pore waters down to 5 m depth throughout one year. The samplers were either located close to the tidal flat margin or in central parts of the tidal flat. Despite dynamic sedimentological and hydrological conditions, the general trends with depth in deep tidal flat pore waters are remarkably similar to those observed in deep sea environments. Rates of trace metal cycling must be comparably large in order to maintain the observed pore water profiles. Trace metals further show similar general trends with depth close to the margin and in central parts of the tidal flat. Seasonal sampling revealed that V and Cr vary concurrent with seasonal changes in dissolved organic carbon (DOC) concentration. This effect is most notable close to the tidal flat margin where sulphate, DOC, and nutrients vary with season down to some metres depth. Seasonal variations of Mn, Fe, Mo, and U are by contrast limited to the upper decimetres of the sediment. Their seasonal patterns depend on organic matter supply, redox stratification, and particulate matter deposited on sediment surfaces. Pore water sampling within one tidal cycle provides evidence for pore water advection in margin sediments. During low tide pore water flow towards the creekbank is generated by a hydraulic gradient suggesting that deep pore waters may be seeping out of creekbank sediments. Owing to the enrichment of specific elements like Mn in pore water compared to sea water, seeping pore waters may have an impact on the chemistry of the open water column. Mass balance calculations reveal that the impact of deep pore waters on the Mn budget in the open water column is below 4%. Mn deep pore

  12. High Frequency Monitoring of the Aigion Fault Activity

    NASA Astrophysics Data System (ADS)

    Cornet, Francois; Bourouis, Seid

    2013-04-01

    In 2007, a high frequency monitoring system was deployed in the 1000 m deep AIG10 well that intersects the Aigion fault at a depth of 760 m. This active 15 km long fault is located on the south shore of the Corinth rift, some 40 km east from Patras, in western central Greece. The borehole intersects quaternary sediments down to 495 m, then cretaceous and tertiary heavily tectonized deposits from the Pindos nappe. Below the fault encountered at 760 m, the borehole remains within karstic limestone of the Gavrovo Tripolitza nappe. The monitoring system involved two geophones located some 15 m above the fault, and two hydrophones located respectively at depths equal to 500 m and 250 m. The frequency domain for the data acquisition system ranged from a few Hz to 2500 Hz. The seismic velocity structure close to the borehole was determined through both sonic logs and vertical seismic profiles. This monitoring system has been active during slightly over six months and has recorded signals from microseismic events that occurred in the rift, the location of which was determined thanks to the local 11 stations, three components, short period (2 Hz), monitoring system. In addition, the borehole monitoring system has recorded more than 1000 events not identified with the regional network. Events were precisely correlated with pressure variations associated with two human interventions. These extremely low magnitude events occurred at distances that reached at least up to 1500 m from the well. They were associated, some ten days later, with some local rift activity. A tentative model is proposed that associates local short slip instabilities in the upper part of the fault close to the well, with a longer duration pore pressure diffusion process. Results demonstrate that the Aigion fault is continuously creeping down to a depth at least equal to 5 km but probably deeper.

  13. Effects of high frequency current in welding aluminum alloy 6061

    NASA Technical Reports Server (NTRS)

    Fish, R. E.

    1968-01-01

    Uncontrolled high frequency current causes cracking in the heat-affected zone of aluminum alloy 6061 weldments during tungsten inert gas ac welding. Cracking developed when an improperly adjusted superimposed high frequency current was agitating the semimolten metal in the areas of grain boundary.

  14. Monitoring method and apparatus using high-frequency carrier

    DOEpatents

    Haynes, H.D.

    1996-04-30

    A method and apparatus for monitoring an electrical-motor-driven device by injecting a high frequency carrier signal onto the power line current. The method is accomplished by injecting a high frequency carrier signal onto an AC power line current. The AC power line current supplies the electrical-motor-driven device with electrical energy. As a result, electrical and mechanical characteristics of the electrical-motor-driven device modulate the high frequency carrier signal and the AC power line current. The high frequency carrier signal is then monitored, conditioned and demodulated. Finally, the modulated high frequency carrier signal is analyzed to ascertain the operating condition of the electrical-motor-driven device. 6 figs.

  15. Monitoring method and apparatus using high-frequency carrier

    DOEpatents

    Haynes, Howard D.

    1996-01-01

    A method and apparatus for monitoring an electrical-motor-driven device by injecting a high frequency carrier signal onto the power line current. The method is accomplished by injecting a high frequency carrier signal onto an AC power line current. The AC power line current supplies the electrical-motor-driven device with electrical energy. As a result, electrical and mechanical characteristics of the electrical-motor-driven device modulate the high frequency carrier signal and the AC power line current. The high frequency carrier signal is then monitored, conditioned and demodulated. Finally, the modulated high frequency carrier signal is analyzed to ascertain the operating condition of the electrical-motor-driven device.

  16. Redox conditions and trace metal cycling in coastal sediments from the maritime Antarctic

    NASA Astrophysics Data System (ADS)

    Monien, Patrick; Lettmann, Karsten Alexander; Monien, Donata; Asendorf, Sanja; Wölfl, Anne-Cathrin; Lim, Chai Heng; Thal, Janis; Schnetger, Bernhard; Brumsack, Hans-Jürgen

    2014-09-01

    Redox-sensitive trace metals (Mn, Fe, U, Mo, Re), nutrients and terminal metabolic products (NO3-, NH4+, PO43-, total alkalinity) were investigated for the first time in pore waters of Antarctic coastal sediments. The results of this study reveal a high spatial variability in redox conditions in surface sediments from Potter Cove, King George Island, western Antarctic Peninsula. Particularly in the shallower areas of the bay the significant correlation between sulphate depletion and total alkalinity, the inorganic product of terminal metabolism, indicates sulphate reduction to be the major pathway of organic matter mineralisation. In contrast, dissimilatory metal oxide reduction seems to be prevailing in the newly ice-free areas and the deeper troughs, where concentrations of dissolved iron of up to 700 μM were found. We suggest a combination of several factors to be responsible for the domination of metal oxide reduction over sulphate reduction in these areas. These include the increased accumulation of fine-grained material with high amounts of reducible metal oxides, a reduced availability of metabolisable organic matter and an enhanced physical and biological disturbance by bottom water currents, ice scouring and burrowing organisms. Based on modelled iron fluxes we calculate the contribution of the Antarctic shelf to the pool of potentially bioavailable iron (Feb) to be 6.9 × 103 to 790 × 103 t yr-1. Consequently, these shelf sediments would provide an Feb flux of 0.35-39.5 mg m-2 yr-1 (median: 3.8 mg m-2 yr-1) to the Southern Ocean. This contribution is in the same order of magnitude as the flux provided by icebergs and significantly higher than the input by aeolian dust. For this reason suboxic shelf sediments form a key source of iron for the high nutrient-low chlorophyll (HNLC) areas of the Southern Ocean. This source may become even more important in the future due to rising temperatures at the WAP accompanied by enhanced glacier retreat and the

  17. Nitrogen cycling between sediment and the shallow-water column in the transition zone of the Potomac River and estuary. I. Nitrate and ammonium fluxes

    NASA Astrophysics Data System (ADS)

    Simon, N. S.

    1988-05-01

    A three-year study of seasonal variation in water-column and sediment nitrogen species was conducted in the transition zone of the Potomac River 35 m from the Virginia shore at a site with an average water-column depth of approximately 1 m over sandy sediment. A diffusion-controlled sampler was used to collect water samples from the water column, at the interface between the water column and sediment, and at several tens of centimeters into the sediment. Nitrate was the predominant dissolved nitrogen species in the water column. The importance of denitrification was inferred by nitrate fluxes which were directed into the sediment from the water column during approximately 75% of the sampling periods and ranged from 0·02 to 0·69 mmol m -2 day -1. Flux of nitrate from the sediment into the water column, ⩾0·1 mmol m -2 day -1, due possibly to nitrification in surficial sediment, occurred during one spring and two summer sampling periods. Ammonium fluxes were less than 0·1 mmol m -2 day -1 during 90% of the sampling periods. Of the ammonium fluxes that were >0·05 mmol m -2 day -1, all were fluxes into the sediment during sampling periods when sediment resuspension occurred, and all were into the water column during periods of calm. The mean value of ammonium flux (0·005 ± 0·05 mmol m -2 day -1) from the sandy, shallow-water sediments was two orders of magnitude less than the ammonium fluxes from the deeper, silty channel sediments in the same reach of the river. Diffusive flux calculations suggest that approximately one order of magnitude more nitrate than ammonium is cycled between the shallow-water column and the sandy sediment in the transition zone of the Potomac River.

  18. Nitrogen cycling between sediment and the shallow-water column in the transition zone of the Potomac River and estuary. I. Nitrate and ammonium fluxes

    USGS Publications Warehouse

    Simon, N.S.

    1988-01-01

    A three-year study of seasonal variation in water-column and sediment nitrogen species was conducted in the transition zone of the Potomac River 35 m from the Virginia shore at a site with an average water-column depth of approximately 1 m over sandy sediment. A diffusion-controlled sampler was used to collect water samples from the water column, at the interface between the water column and sediment, and at several tens of centimeters into the sediment. Nitrate was the predominant dissolved nitrogen species in the water column. The importance of denitrification was inferred by nitrate fluxes which were directed into the sediment from the water column during approximately 75% of the sampling periods and ranged from 0??02 to 0??69 mmol m-2 day-1. Flux of nitrate from the sediment into the water column, ???0??1 mmol m-2 day-1, due possibly to nitrification in surficial sediment, occurred during one spring and two summer sampling periods. Ammonium fluxes were less than 0??1 mmol m-2 day-1 during 90% of the sampling periods. Of the ammonium fluxes that were >0??05 mmol m-2 day-1, all were fluxes into the sediment during sampling periods when sediment resuspension occurred, and all were into the water column during periods of calm. The mean value of ammonium flux (0??005 ?? 0??05 mmol m-2 day-1) from the sandy, shallow-water sediments was two orders of magnitude less than the ammonium fluxes from the deeper, silty channel sediments in the same reach of the river. Diffusive flux calculations suggest that approximately one order of magnitude more nitrate than ammonium is cycled between the shallow-water column and the sandy sediment in the transition zone of the Potomac River. ?? 1988.

  19. High-frequency cyclicity in the latest Messinian Adriatic foreland basin: Insight into palaeoclimate and palaeoenvironments of the Mediterranean Lago-Mare episode

    NASA Astrophysics Data System (ADS)

    Cosentino, D.; Cipollari, P.; Lo Mastro, S.; Giampaolo, C.

    2005-07-01

    Late Messinian Lago-Mare deposits show high-frequency cyclicity in the whole Mediterranean Basin. Both millimeter- and centimeter-scale cyclicities have been observed in several ODP sites as well as in stratigraphic sections from the Mediterranean borderland. We have analyzed a well-exposed late Messinian Lago-Mare section from the Adriatic side of the central Apennines (Italy). At the Fonte dei Pulcini section (SE Majella Mts.), millimeter- and centimeter-scale white-and-dark couplets have been observed in the field. A 50 cm regular-spaced sampling has been performed in the uppermost 53 m of the late Messinian Lago-Mare clays. On the 107 collected samples, geochemical (CaCO 3 content), mineralogical (XRD analyses), and micropalaeontological investigations have been performed. In addition, SEM and microprobe investigations as well as mineralogical and micropalaeontological analyses have been carried out on single lamina from a 34-cm-thick interval of millimeter-scale laminites. Besides the 10 3 cycles/m and 10 2 cycles/m frequencies observed in the field, spectral analyses performed on the CaCO 3 data set indicated other high-frequency cyclicities: 0.47 cycles/m, 0.35 cycles/m, and 0.17 cycles/m. Taking into account the estimated sedimentation rate, these frequencies correspond, respectively, to periodicities of: 1 year, 10 years, 2.1 kyr, 2.8 kyr, and 5.6 kyr. These sub-Milankovitch cyclicities have been related to annual and sunspot solar activity. The millimeter-scale couplets are interpreted as varves sedimented in an ephemeral water environment marginal to a perennial brackish water lagoonal basin. These varved sediments reflect a marked seasonality characterized by the alternation of arid and more humid climatic phases. The presence of high values of smectite (60-80%) in the clay minerals of the analyzed samples could be a consequence of these climatic oscillations from drier to moister conditions. The climatic scenario suggested in this paper for the late

  20. Polluted harbor sediment and the annual reproductive cycle of the female flounder, Platichthys fiesus (L.)

    SciTech Connect

    Janssen, P.A.H.; Lambert, J.G.D.; Goos, H.J.T.; Wezel, A.P. van; Opperhuizen, A.

    1995-12-31

    Compounds such as polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs),and pesticides are metabolized by enzyme systems, which are also involved in steroid metabolism. Disturbances of reproduction may therefore occur through the interference of these compounds with the endocrine system. Several aspects of reproduction were studied in the flounder, Platichthys fiesus (L.), an euryhaline flatfish which inhabits coastal waters and is therefore a suitable biomonitor for the effects of chemical pollutants. Fish were kept during three years in mesocosm systems of which the first provided a control, while the second one contained polluted sediment, derived from the Rotterdam harbor. In November, all ovaries from both mesocosms contained vitellogenic oocytes. In May, all the control fish were previtellogenic, while the ovaries of fish from the polluted mesocosm contained, besides previtellogenic oocytes, a large number of vitellogenic oocytes, indicating that an estrogenic induction had occurred. The in vitro tissue incubations with androstenedione as precursor revealed that the ovarian capacity to synthesize testosterone (T), estrone (E{sub 2}) and 17{beta}-estradiol (E{sub 2}) didn`t differ between both mesocosms. In May, however, the levels of T and E{sub 2} as well as the level of the yolk-precursor vitellogenin were significantly higher in the polluted mesocosm. The conclusion from this study was that polluted harbor sediment contains compounds that effect normal reproductive development, i.e. the induction of premature vitellogenesis.

  1. Acid-Tolerant Sulfate-Reducing Bacteria Play a Major Role in Iron Cycling in Acidic Iron Rich Sediments

    NASA Astrophysics Data System (ADS)

    Enright, K. A.; Moreau, J. W.

    2008-12-01

    Climate change drives drying and acidification of many rivers and lakes. Abundant sedimentary iron in these systems oxidizes chemically and biologically to form iron-ox(yhydrox)ide crusts and "hardpans". Given generally high sulfate concentrations, the mobilization and cycling of iron in these environments can be strongly influenced by bacterial sulfate reduction. Sulfate-reducing bacteria (SRB) induce reductive dissolution of oxidized iron phases by producing the reductant bisulfide as a metabolic product. These environmentally ubiquitous microbes also recycle much of the fixed carbon in sediment-hosted microbial mat communities. With prevalent drying, the buffering capacity for protons liberated from iron oxidation is exceeded, and the activity of sulfate-reducers is restricted to those species capable of tolerating low pH (and generally highly saline, i.e. sulfate-rich) conditions. These species will sustain the recycling of iron from more crystalline phases to more bioavailable species, as well as act as the only source of bisulfide for photosynthesizing microbial communities. The phylogeny and physiology of acid-tolerant SRB is therefore important to Fe, S and C cycling in iron-rich sedimentary environments, particularly those on a geochemical trajectory towards acidification. Previous studies have shown that these SRB species tend to be highly novel. We studied two distinct environments along a geochemical continuum towards acidification. In both settings, iron redox transformations exert a major, if not controlling, influence on reduction potential. An acidified, iron- rich tidal marsh receiving acid-mine drainage (San Francisco Bay, CA, USA) contained abundant textural evidence for reductive dissolution of Fe(III) in sediments with pH values varying from 2.4 - 3.8. From these sediments, full-length novel dsrAB gene sequences from acid-tolerant SRB were recovered, and sulfur isotope profiles reflected biological fractionation of sulfur under even the most

  2. Sedimentation in Lake El'gygytgyn, Northeastern Siberia, During the Past Three Climate Cycles

    NASA Astrophysics Data System (ADS)

    Melles, M.; Anderson, P. M.; Apfelbaum, M.; Asikainen, C.; Brigham-Grette, J.; Cherepanova, M.; Kopsch, C.; Forman, S.; Juschus, O.; Lozhkin, A. V.; Minyuk, P.; Niessen, F.; Nowaczyk, N. R.; Snyder, J.

    2004-12-01

    Lake El'gygytgyn, located in central Chukotka, NE Siberia, is a 3.6 million year old impact crater lake with a diameter of 12 km and a water depth of 170 m. Sediment cores of 13 and 16 m length from the deepest part of the lake were investigated for chronology, physical properties, sedimentology, biogeochemistry, inorganic geochemistry, mineralogy, palynology and diatom assemblages. The cores are undisturbed and complete. With basal ages of approx. 250 and 300 ka, respectively, they represent the longest continuous climate records as yet available from the Arctic continent. Besides two ash layers and a number of distal, fine-grained turbidites, four "pelagic" sediment units of different composition can be distinguished. During warm periods (units 1 and 2), summer melt of the ice cover leads to high aquatic primary production, ventilation of the entire water column by annual turnover, decomposition of most of the settling particulate organic matter, and bioturbation of the sediments. Among these periods, MIS 5.5 (Eemian) and to a smaller degree late MIS 1 (Holocene) exhibited significantly higher organic matter accumulation (unit 1) than MIS 3, 5.1, 5.3, 6.1, 6.3, 6.5, 7.1, 7.3, and 7.5 (unit 2). This was probably due to enhanced nutrient and organic matter supply from a more dense vegetation cover in the catchment. The density and composition of the regional vegetation, in turn, was not only controlled by insolation, but also by precipitation and/or temperature changes due to alterations in the atmospheric circulation pattern. During cold periods (units 3 and 4), a persistent ice cover hampers primary production and leads to a stratified water column with anoxic bottom waters, good preservation of the settling organic matter, and the formation of laminated sediments due to the absence of bioturbating organisms. Among these periods, cold and particularly dry climates lead to the widespread absence of blanketing snow on the lake ice cover, enabling the formation of

  3. Impact of glacial/interglacial changes in water column geochemistry on the diagenetic cycling of barium in Black Sea sediments

    NASA Astrophysics Data System (ADS)

    Kasten, S.; Henkel, S.; Mogollón, J. M.; Nöthen, K.; Franke, C.; Bogus, K.; Robin, E.; Bahr, A.; Blumenberg, M.; Pape, T.; Seifert, R.; Marz, C.; De Lange, G. J.

    2012-12-01

    Changes in depositional conditions and redox environment over time affect biogeochemical processes in the seabed and in this way control the variable and selective preservation, alteration and formation of various sediment constituents and attributes - including particulate organic matter, mineral assemblages and magnetic properties. As many of these solid-phase compounds are used as paleo-environmental tracers or stratigraphic tools an assessment of diagenetic influences on the sedimentary record is crucial for accurate environmental reconstructions. We present an integrated approach of pore-water and solid-phase geochemistry as well as transport reaction modeling for sediments of the Black Sea to assess the biogeochemical history of these deposits with particular emphasis on post-depositional redistribution of barium as a consequence of changes in water column geochemistry and redox (Henkel et al., 2012). High-resolution sedimentary records of major and minor elements (Al, Ba, Ca, Sr, Ti), total organic carbon (TOC), and profiles of pore-water constituents (SO42-, CH4, Ca2+, Ba2+, Mg2+, alkalinity) were obtained for two gravity cores (core 755, 501 m water depth and core 214, 1686 m water depth) from the northwestern Black Sea. The records were examined in order to gain insight into the cycling of Ba in anoxic marine sediments characterized by a shallow sulfate-methane transition (SMT) as well as the applicability of barite as a primary productivity proxy in such a setting. The Ba records are strongly overprinted by diagenetic barite (BaSO4) remobilization and precipitation; authigenic Ba enrichments were found at both sites at and slightly above the current SMT. Transport reaction modeling was applied to simulate the migration of the SMT during the changing geochemical conditions after the Holocene seawater intrusion into the Black Sea. Based on this, sediment intervals affected by diagenetic Ba redistribution were identified. Results reveal that the intense

  4. Variation in numbers and behaviour of waders during the tidal cycle: implications for the use of estuarine sediment flats

    NASA Astrophysics Data System (ADS)

    Granadeiro, José P.; Dias, Maria P.; Martins, Ricardo C.; Palmeirim, Jorge M.

    2006-05-01

    Estuarine sediment flats are essential feeding areas for waders, but their exploitation is constrained by the movements of tides. In this cyclic environment the exposure period of sediment flats decreases several fold from upper to lower flats, and the moving tidal waterline briefly creates particular conditions for waders and their prey. This study attempts to determine how the exposure period and the movement of the tide line influence the use of space and food resources by waders across the sediment flats. Wader counts and observations of feeding behaviour were carried out in all phases of the tidal cycle, in plots forming a transect from upper to lower flats, thus representing a gradient of exposure periods. Pecking, prey intake, and success rates varied little along the gradient. Some species actively followed the tide line while foraging, whereas others are evenly spread over the exposed flats. Black-tailed Godwit, Dunlin and Avocet were 'tide followers', whereas Grey Plover, Redshank and Bar-tailed Godwit were 'non-followers'. Densities of 'followers' near the tide line were up to five times higher than elsewhere. Species differed markedly in the way they used space on the flats, but in general the rate of biomass acquisition (in grams of ash-free dry weight per time exposed) was much higher in lower flats. However, this preference was insufficient to counter the much longer exposure of the upper flats, so the total amount of biomass consumed on the latter was greater. Therefore, it was in these upper flats that waders fulfilled most of their energetic needs. Consequently, upper flats are of particular importance for the conservation of wader assemblages, but because they are usually closer to shore they tend to suffer the highest pressure from disturbance and land reclamation.

  5. Mercury cycling and sequestration in salt marshes sediments: an ecosystem service provided by Juncus maritimus and Scirpus maritimus.

    PubMed

    Marques, B; Lillebø, A I; Pereira, E; Duarte, A C

    2011-07-01

    In this study two time scales were looked at: a yearlong study was completed, and a 180-day decay experiment was done. Juncus maritimus and Scirpus maritimus have different life cycles, and this seems to have implications in the Hg-contaminated salt marsh sediment chemical environment, namely Eh and pH. In addition, the belowground biomass decomposition rates were faster for J. maritimus, as well as the biomass turnover rates. Results show that all these species-specific factors have implications in the mercury dynamics and sequestration. Meaning that J. maritimus belowground biomass has a sequestration capacity for mercury per square metre approximately 4-5 times higher than S. maritimus, i.e., in S. maritimus colonized areas Hg is more extensively exchange between belowground biomass and the rhizosediment. In conclusion, J. maritimus seems to provide a comparatively higher ecosystem service through phytostabilization (Hg complexation in the rhizosediment) and through phytoaccumulation (Hg sequestration in the belowground biomass). PMID:21514707

  6. Hydrologic controls on nitrogen cycling processes and functional gene abundance in sediments of a groundwater flow-through lake

    USGS Publications Warehouse

    Stoliker, Deborah L.; Repert, Deborah A.; Smith, Richard L.; Song, Bongkeun; LeBlanc, Denis R.; McCobb, Timothy D.; Conaway, Christopher; Hyun, Sung Pil; Koh, Dong-Chan; Moon, Hee Sun; Kent, Douglas B.

    2016-01-01

    The fate and transport of inorganic nitrogen (N) is a critically important issue for human and aquatic ecosystem health because discharging N-contaminated groundwater can foul drinking water and cause algal blooms. Factors controlling N-processing were examined in sediments at three sites with contrasting hydrologic regimes at a lake on Cape Cod, MA. These factors included water chemistry, seepage rates and direction of groundwater flow, and the abundance and potential rates of activity of N-cycling microbial communities. Genes coding for denitrification, anaerobic ammonium oxidation (anammox), and nitrification were identified at all sites regardless of flow direction or groundwater dissolved oxygen concentrations. Flow direction was, however, a controlling factor in the potential for N-attenuation via denitrification in the sediments. Potential rates of denitrification varied from 6 to 4500 pmol N/g/h from the inflow to the outflow side of the lake, owing to fundamental differences in the supply of labile organic matter. The results of laboratory incubations suggested that when anoxia and limiting labile organic matter prevailed, the potential existed for concomitant anammox and denitrification. Where oxic lake water was downwelling, potential rates of nitrification at shallow depths were substantial (1640 pmol N/g/h). Rates of anammox, denitrification, and nitrification may be linked to rates of organic N-mineralization, serving to increase N-mobility and transport downgradient.

  7. Hydrologic Controls on Nitrogen Cycling Processes and Functional Gene Abundance in Sediments of a Groundwater Flow-Through Lake.

    PubMed

    Stoliker, Deborah L; Repert, Deborah A; Smith, Richard L; Song, Bongkeun; LeBlanc, Denis R; McCobb, Timothy D; Conaway, Christopher H; Hyun, Sung Pil; Koh, Dong-Chan; Moon, Hee Sun; Kent, Douglas B

    2016-04-01

    The fate and transport of inorganic nitrogen (N) is a critically important issue for human and aquatic ecosystem health because discharging N-contaminated groundwater can foul drinking water and cause algal blooms. Factors controlling N-processing were examined in sediments at three sites with contrasting hydrologic regimes at a lake on Cape Cod, MA. These factors included water chemistry, seepage rates and direction of groundwater flow, and the abundance and potential rates of activity of N-cycling microbial communities. Genes coding for denitrification, anaerobic ammonium oxidation (anammox), and nitrification were identified at all sites regardless of flow direction or groundwater dissolved oxygen concentrations. Flow direction was, however, a controlling factor in the potential for N-attenuation via denitrification in the sediments. Potential rates of denitrification varied from 6 to 4500 pmol N/g/h from the inflow to the outflow side of the lake, owing to fundamental differences in the supply of labile organic matter. The results of laboratory incubations suggested that when anoxia and limiting labile organic matter prevailed, the potential existed for concomitant anammox and denitrification. Where oxic lake water was downwelling, potential rates of nitrification at shallow depths were substantial (1640 pmol N/g/h). Rates of anammox, denitrification, and nitrification may be linked to rates of organic N-mineralization, serving to increase N-mobility and transport downgradient. PMID:26967929

  8. The multiproxy analysis of a lacustrine-palustrine sediment core from Lebanon reveals four climate cycles

    NASA Astrophysics Data System (ADS)

    Gasse, F. A.; van Campo, E.; Demory, F.; Develle, A.; Tachikawa, K.; Buchet, N.; Sonzogni, C.; Thouveny, N.; Bard, E. G.; Vidal, L.

    2013-12-01

    The study of a sediment core retrieved from the small Yammouneh basin (34.06°N-34.09°N, 36.0°E-36.03°E, 1360 m a.s.l.), Lebanon, provides for the first time a nearly continuous record spanning approximately 360 ka in northern Levant. The basin, located on the eastern flank of Mount Lebanon, is mainly supplied by karstic springs which discharge snowmelt water infiltrated through the western highlands. Part of its water inputs is lost by seepage through its faulted bottom. The core, 73 m long, consists of four whitish carbonated intervals rich in lacustrine organism remains, interrupting a thick accumulation of colored silty clays almost devoid of shells but for ostracods. We analyzed sediment features (mineralogical and elemental composition, light microscopy and SEM observations, grain size), magnetic properties, pollen and calcite oxygen isotopes (δc) derived from ostracod shell composition. The chronological framework is based on 14C ages of wood fragments, U/Th dating, and a high resolution reconstruction of relative paleointensity variations correlated with regional (Portuguese margin) and global (Sint-800) master curves down to about 360 ka. Although the chronology still needs improvement, the 3 upper carbonated intervals undoubtedly fit Interglacials MIS 1, MIS 5.5 and MIS 7, respectively. The deepest one (49-60 m) is assigned to MIS 9 by its proxy analogies with dated Interglacials. The sequence covers a large part of MIS 10. Relationships between individual indicators are explored, in addition to visual comparisons of individual records, from the multiproxy matrix after resampling at a common depth scale of 25 cm. We compute simple linear coefficients between 20 variables, perform Principal Component Analyses based on all variables, on terrestrial pollen biomes, on all sedimentological proxies, and cross-correlations between them and δc. During Interglacial maxima, high local and regional efficient moisture is evidenced by dense arboreal vegetation of

  9. The effects of hypoxia on sediment nitrogen cycling in the Baltic Sea.

    PubMed

    Jäntti, Helena; Hietanen, Susanna

    2012-03-01

    Primary production in the eutrophic Baltic Sea is limited by nitrogen availability; hence denitrification (natural transformation of nitrate to gaseous N(2)) in the sediments is crucial in mitigating the effects of eutrophication. This study shows that dissimilatory nitrate reduction to ammonium (DNRA) process, where nitrogen is not removed but instead recycled in the system, dominates nitrate reduction in low oxygen conditions (O(2) <110 μM), which have been persistent in the central Gulf of Finland during the past decade. The nitrogen removal rates measured in this study show that nitrogen removal has decreased in the Gulf of Finland compared to rates measured in mid-1990s and the decrease is most likely caused by the increased bottom water hypoxia. PMID:22246635

  10. Anodes Stimulate Anaerobic Toluene Degradation via Sulfur Cycling in Marine Sediments

    PubMed Central

    Daghio, Matteo; Vaiopoulou, Eleni; Patil, Sunil A.; Suárez-Suárez, Ana; Head, Ian M.

    2015-01-01

    Hydrocarbons released during oil spills are persistent in marine sediments due to the absence of suitable electron acceptors below the oxic zone. Here, we investigated an alternative bioremediation strategy to remove toluene, a model monoaromatic hydrocarbon, using a bioanode. Bioelectrochemical reactors were inoculated with sediment collected from a hydrocarbon-contaminated marine site, and anodes were polarized at 0 mV and +300 mV (versus an Ag/AgCl [3 M KCl] reference electrode). The degradation of toluene was directly linked to current generation of up to 301 mA m−2 and 431 mA m−2 for the bioanodes polarized at 0 mV and +300 mV, respectively. Peak currents decreased over time even after periodic spiking with toluene. The monitoring of sulfate concentrations during bioelectrochemical experiments suggested that sulfur metabolism was involved in toluene degradation at bioanodes. 16S rRNA gene-based Illumina sequencing of the bulk anolyte and anode samples revealed enrichment with electrocatalytically active microorganisms, toluene degraders, and sulfate-reducing microorganisms. Quantitative PCR targeting the α-subunit of the dissimilatory sulfite reductase (encoded by dsrA) and the α-subunit of the benzylsuccinate synthase (encoded by bssA) confirmed these findings. In particular, members of the family Desulfobulbaceae were enriched concomitantly with current production and toluene degradation. Based on these observations, we propose two mechanisms for bioelectrochemical toluene degradation: (i) direct electron transfer to the anode and/or (ii) sulfide-mediated electron transfer. PMID:26497463