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Sample records for high-resolution optical recording

  1. High-resolution non-contact measurement of the electrical activity of plants in situ using optical recording.

    PubMed

    Zhao, Dong-Jie; Chen, Yang; Wang, Zi-Yang; Xue, Lin; Mao, Tong-Lin; Liu, Yi-Min; Wang, Zhong-Yi; Huang, Lan

    2015-01-01

    The limitations of conventional extracellular recording and intracellular recording make high-resolution multisite recording of plant bioelectrical activity in situ challenging. By combining a cooled charge-coupled device camera with a voltage-sensitive dye, we recorded the action potentials in the stem of Helianthus annuus and variation potentials at multiple sites simultaneously with high spatial resolution. The method of signal processing using coherence analysis was used to determine the synchronization of the selected signals. Our results provide direct visualization of the phloem, which is the distribution region of the electrical activities in the stem and leaf of H. annuus, and verify that the phloem is the main action potential transmission route in the stems of higher plants. Finally, the method of optical recording offers a unique opportunity to map the dynamic bioelectrical activity and provides an insight into the mechanisms of long-distance electrical signal transmission in higher plants. PMID:26333536

  2. High-resolution non-contact measurement of the electrical activity of plants in situ using optical recording

    PubMed Central

    Zhao, Dong-Jie; Chen, Yang; Wang, Zi-Yang; Xue, Lin; Mao, Tong-Lin; Liu, Yi-Min; Wang, Zhong-Yi; Huang, Lan

    2015-01-01

    The limitations of conventional extracellular recording and intracellular recording make high-resolution multisite recording of plant bioelectrical activity in situ challenging. By combining a cooled charge-coupled device camera with a voltage-sensitive dye, we recorded the action potentials in the stem of Helianthus annuus and variation potentials at multiple sites simultaneously with high spatial resolution. The method of signal processing using coherence analysis was used to determine the synchronization of the selected signals. Our results provide direct visualization of the phloem, which is the distribution region of the electrical activities in the stem and leaf of H. annuus, and verify that the phloem is the main action potential transmission route in the stems of higher plants. Finally, the method of optical recording offers a unique opportunity to map the dynamic bioelectrical activity and provides an insight into the mechanisms of long-distance electrical signal transmission in higher plants. PMID:26333536

  3. Holographic high-resolution endoscopic image recording

    NASA Astrophysics Data System (ADS)

    Bjelkhagen, Hans I.

    1991-03-01

    Endoscopic holography or endoholography combines the features of endoscopy and holography. The purpose of endoholographic imaging is to provide the physician with a unique means of extending diagnosis by providing a life-like record of tissue. Endoholographic recording will provide means for microscopic examination of tissue and in some cases may obviate the need to excise specimens for biopsy. In this method holograms which have the unique properties of three-dimensionality large focal depth and high resolution are made with a newly designed endoscope. The endoscope uses a single-mode optical fiber for illumination and single-beam reflection holograms are recorded in close contact with the tissue at the distal end of the endoscope. The holograms are viewed under a microscope. By using the proper combinations of dyes for staining specific tissue types with various wavelengths of laser illumination increased contrast on the cellular level can be obtained. Using dyes such as rose bengal in combination with the 514. 5 nm line of an argon ion laser and trypan blue or methylene blue with the 647. 1 nm line of a krypton ion laser holograms of the stained colon of a dog showed the architecture of the colon''s columnar epithelial cells. It is hoped through chronological study using this method in-vivo an increased understanding of the etiology and pathology of diseases such as Crohn''s diseases colitis proctitis and several different forms of cancer will help

  4. High resolution optical DNA mapping

    NASA Astrophysics Data System (ADS)

    Baday, Murat

    Many types of diseases including cancer and autism are associated with copy-number variations in the genome. Most of these variations could not be identified with existing sequencing and optical DNA mapping methods. We have developed Multi-color Super-resolution technique, with potential for high throughput and low cost, which can allow us to recognize more of these variations. Our technique has made 10--fold improvement in the resolution of optical DNA mapping. Using a 180 kb BAC clone as a model system, we resolved dense patterns from 108 fluorescent labels of two different colors representing two different sequence-motifs. Overall, a detailed DNA map with 100 bp resolution was achieved, which has the potential to reveal detailed information about genetic variance and to facilitate medical diagnosis of genetic disease.

  5. Wavefront metrology for high resolution optical systems

    NASA Astrophysics Data System (ADS)

    Miyakawa, Ryan H.

    Next generation extreme ultraviolet (EUV) optical systems are moving to higher resolution optics to accommodate smaller length scales targeted by the semiconductor industry. As the numerical apertures (NA) of the optics become larger, it becomes increasingly difficult to characterize aberrations due to experimental challenges associated with high-resolution spatial filters and geometrical effects caused by large incident angles of the test wavefront. This dissertation focuses on two methods of wavefront metrology for high resolution optical systems. The first method, lateral shearing interferometry (LSI), is a self-referencing interferometry where the test wavefront is incident on a low spatial frequency grating, and the resulting interference between the diffracted orders is used to reconstruct the wavefront aberrations. LSI has many advantages over other interferometric tests such as phase-shifting point diffraction interferometry (PS/PDI) due to its experimental simplicity, stability, relaxed coherence requirements, and its ability to scale to high numerical apertures. While LSI has historically been a qualitative test, this dissertation presents a novel quantitative investigation of the LSI interferogram. The analysis reveals the existence of systematic aberrations due to the nonlinear angular response from the diffraction grating that compromises the accuracy of LSI at medium to high NAs. In the medium NA regime (0.15 < NA < 0.35), a holographic model is presented that derives the systematic aberrations in closed form, which demonstrates an astigmatism term that scales as the square of the grating defocus. In the high NA regime (0.35 < NA), a geometrical model is introduced that describes the aberrations as a system of transcendental equations that can be solved numerically. The characterization and removal of these systematic errors is a necessary step that unlocks LSI as a viable candidate for high NA EUV optical testing. The second method is a novel image

  6. High resolution bragg focusing optics for synchrotron monochromators and analyzers

    SciTech Connect

    Knapp, G.S.; Beno, M.A.; Gofron, K.J.

    1997-07-01

    A number of different applications for high resolution Bragg Focusing Optics are reviewed. Applications include Sagittal Focusing, Energy Dispersive optics for x-ray absorption and diffraction, a curved analyzer-multichannel detector method for efficient acquisition of powder and small angle scattering data, the use of Backscattering Analyzers for very high resolution inelastic scattering, and curved crystals for high energy applications.

  7. Invariant high resolution optical skin imaging

    NASA Astrophysics Data System (ADS)

    Murali, Supraja; Rolland, Jannick

    2007-02-01

    Optical Coherence Microscopy (OCM) is a bio-medical low coherence interferometric imaging technique that has become a topic of active research because of its ability to provide accurate, non-invasive cross-sectional images of biological tissue with much greater resolution than the current common technique ultrasound. OCM is a derivative of Optical Coherence Tomography (OCT) that enables greater resolution imposed by the implementation of an optical confocal design involving high numerical aperture (NA) focusing in the sample. The primary setback of OCM, however is the depth dependence of the lateral resolution obtained that arises from the smaller depth of focus of the high NA beam. We propose to overcome this limitation using a dynamic focusing lens design that can achieve quasi-invariant lateral resolution up to 1.5mm depth of skin tissue.

  8. Optical alignment of high resolution Fourier transform spectrometers

    NASA Technical Reports Server (NTRS)

    Breckinridge, J. B.; Ocallaghan, F. G.; Cassie, A. G.

    1980-01-01

    Remote sensing, high resolution FTS instruments often contain three primary optical subsystems: Fore-Optics, Interferometer Optics, and Post, or Detector Optics. We discuss the alignment of a double-pass FTS containing a cat's-eye retro-reflector. Also, the alignment of fore-optics containing confocal paraboloids with a reflecting field stop which relays a field image onto a camera is discussed.

  9. Optical multichannel analyzer techniques for high resolution optical spectroscopy

    SciTech Connect

    Chao, J.L.

    1980-06-01

    The development of optical multichannel analyzer techniques for UV/VIS spectroscopy is presented. The research focuses on the development of spectroscopic techniques for measuring high resolution spectral lineshape functions from the exciton phosphorescence in H/sub 2/-1,2,4,5-tetrachlorobenzene. It is found that the temperature dependent frequency shifts and widths confirm a theoretical model based on an exchange theory. The exchange of low energy phonon modes which couple with excited state exciton transitions is shown to display the proper temperature dependent behavior. In addition to the techniques for using the optical multichannel analyzer (OMA) to perform low light level target integration, the use of the OMA for capturing spectral information in transient pulsed laser applications is discussed. An OMP data acquisition system developed for real-time signal processng is described. Both hardware and software interfacing considerations for control and data acquisition by a microcomputer are described. The OMA detector is described in terms of the principles behind its photoelectron detection capabilities and its design is compared with other optoelectronic devices.

  10. High-Resolution Cellulose Oxygen Isotope Records From Indonesian Trees

    NASA Astrophysics Data System (ADS)

    Poussart, P. F.; Schrag, D. P.; Evans, M. N.; D'Arrigo, R. D.

    2002-12-01

    Tropical paleoclimate records such as those derived from ice cores, lake sediments, and corals have yielded valuable insights into the Holocene history of the major modes of climate variability, in particular the El Niño/Southern Oscillation. However, because tropical trees generally lack visible, annual growth rings, their potential as recorders of climate variability has not been fully explored. To circumvent this difficulty, we made high-resolution oxygen isotopic measurements on cellulose extracted from tree samples from Indonesia. First, we examined teak samples, which are one of the rare tropical trees that display well-defined annual growth rings. The samples cover the period from 1800 to the present and show annual to bi-annual cycles, coherent with the visible rings. We use these samples to explore intra-site and intra-tree variability. Second, we measured δ18O time series from three suar wood samples with no visible rings. The oxygen isotopic values are compared to radiocarbon-based age models and show a positive correlation between estimated growth rates and amplitude of the 18O seasonal cycle, suggesting a common environmental forcing. Although the isotopic seasonal cycles are not always regular enough for perfect chronological control, they provide encouraging support for the use of high-resolution isotope records from tropical trees as proxies for multi-century reconstructions of climate variability.

  11. High-resolution adaptive optics test bed for vision science

    NASA Astrophysics Data System (ADS)

    Wilks, Scott C.; Thompson, Charles A.; Olivier, Scot S.; Bauman, Brian J.; Flath, Laurence M.; Silva, Dennis A.; Sawvel, Robert M.; Barnes, Thomas B.; Werner, John S.

    2002-02-01

    We discuss the design and implementation of a low-cost, high-resolution adaptive optics test-bed for vision research. It is well known that high-order aberrations in the human eye reduce optical resolution and limit visual acuity. However, the effects of aberration-free eyesight on vision are only now beginning to be studied using adaptive optics to sense and correct the aberrations in the eye. We are developing a high-resolution adaptive optics system for this purpose using a Hamamatsu Parallel Aligned Nematic Liquid Crystal Spatial Light Modulator. Phase-wrapping is used to extend the effective stroke of the device, and the wavefront sensing and wavefront correction are done at different wavelengths. Issues associated with these techniques will be discussed.

  12. Adaptive optics high resolution spectroscopy: present status and future direction

    SciTech Connect

    Alcock, C; Angel, R; Ciarlo, D; Fugate, R O; Ge, J; Kuzmenko, P; Lloyd-Hart, M; Macintosh, B; Najita, J; Woolf, N

    1999-07-27

    High resolution spectroscopy experiments with visible adaptive optics (AO) telescopes at Starfire Optical Range and Mt. Wilson have demonstrated that spectral resolution can be routinely improved by a factor of - 10 over the seeing-limited case with no extra light losses at visible wavelengths. With large CCDs now available, a very wide wavelength range can be covered in a single exposure. In the near future, most large ground-based telescopes will be equipped with powerful A0 systems. Most of these systems are aimed primarily at diffraction-limited operation in the near IR. An exciting new opportunity will thus open up for high resolution IR spectroscopy. Immersion echelle gratings with much coarser grooves being developed by us at LLNL will play a critical role in achieving high spectral resolution with a compact and low cost IR cryogenically cooled spectrograph and simultaneous large wavelength coverage on relatively small IR detectors. We have constructed a new A0 optimized spectrograph at Steward Observatory to provide R = 200,000 in the optical, which is being commissioned at the Starfire Optical Range 3.5m telescope. We have completed the optical design of the LLNL IR Immersion Spectrograph (LISPEC) to take advantage of improved silicon etching technology. Key words: adaptive optics, spectroscopy, high resolution, immersion gratings

  13. High Resolution Imagery of Haarp-Induced Optical Emissions

    NASA Astrophysics Data System (ADS)

    Kendall, E. A.

    2012-12-01

    One powerful technique for diagnosing radio frequency interactions in the ionosphere is to use ground-based optical instrumentation. High-frequency (HF), heater-induced optical emission observations can be used to diagnose electron energies and distributions in the heated region, illuminate natural and/or artificially induced ionospheric irregularities, determine ExB plasma drifts, and measure quenching rates by neutral species. Optical emissions are caused by HF-accelerated electrons colliding with various atmospheric constituents, which in turn emit a photon. The most common emissions are 630.0 nm O(1D), 557.7 nm O(1S), and 427.8 nm N2+(1NG). Since fairly wide field-of-view imagers are typically deployed in airglow campaigns, it is not well-known what meter-scale features exist in the artificial airglow emissions. Telescopic imaging provides high resolution spatial coverage of ionospheric irregularities and goes hand in hand with other observing techniques such as GPS scintillation, radar, and ionosonde. Imaging can be used to verify the interpretation of data from these other instruments, and this in turn allows confidence in such measurements when airglow cannot be observed (high solar angle or cloud cover). Telescopic imaging of airglow is the only technique capable of simultaneously determining the properties of ionospheric irregularities at decameter resolution over a range of several kilometers. The HAARP telescopic imager consists of two cameras, a set of optics for each camera, and a robotic mount that supports and orients the system. The camera and optics systems are identical except for the camera lenses: one has a wide-angle lens (~19 degrees) and the other has a telescopic lens (~3 degrees). The telescopic imager has a resolution of ~20 m in the F layer and ~10 m in the E layer, which allows the observation of decameter- and kilometer-scale features. Telescopic data has been recorded at HAARP for several years and images will be presented showing

  14. Adaptive optics with pupil tracking for high resolution retinal imaging

    PubMed Central

    Sahin, Betul; Lamory, Barbara; Levecq, Xavier; Harms, Fabrice; Dainty, Chris

    2012-01-01

    Adaptive optics, when integrated into retinal imaging systems, compensates for rapidly changing ocular aberrations in real time and results in improved high resolution images that reveal the photoreceptor mosaic. Imaging the retina at high resolution has numerous potential medical applications, and yet for the development of commercial products that can be used in the clinic, the complexity and high cost of the present research systems have to be addressed. We present a new method to control the deformable mirror in real time based on pupil tracking measurements which uses the default camera for the alignment of the eye in the retinal imaging system and requires no extra cost or hardware. We also present the first experiments done with a compact adaptive optics flood illumination fundus camera where it was possible to compensate for the higher order aberrations of a moving model eye and in vivo in real time based on pupil tracking measurements, without the real time contribution of a wavefront sensor. As an outcome of this research, we showed that pupil tracking can be effectively used as a low cost and practical adaptive optics tool for high resolution retinal imaging because eye movements constitute an important part of the ocular wavefront dynamics. PMID:22312577

  15. A high-resolution record of Greenland mass balance

    NASA Astrophysics Data System (ADS)

    McMillan, Malcolm; Leeson, Amber; Shepherd, Andrew; Briggs, Kate; Armitage, Thomas W. K.; Hogg, Anna; Kuipers Munneke, Peter; Broeke, Michiel; Noël, Brice; Berg, Willem Jan; Ligtenberg, Stefan; Horwath, Martin; Groh, Andreas; Muir, Alan; Gilbert, Lin

    2016-07-01

    We map recent Greenland Ice Sheet elevation change at high spatial (5 km) and temporal (monthly) resolution using CryoSat-2 altimetry. After correcting for the impact of changing snowpack properties associated with unprecedented surface melting in 2012, we find good agreement (3 cm/yr bias) with airborne measurements. With the aid of regional climate and firn modeling, we compute high spatial and temporal resolution records of Greenland mass evolution, which correlate (R = 0.96) with monthly satellite gravimetry and reveal glacier dynamic imbalance. During 2011-2014, Greenland mass loss averaged 269 ± 51 Gt/yr. Atmospherically driven losses were widespread, with surface melt variability driving large fluctuations in the annual mass deficit. Terminus regions of five dynamically thinning glaciers, which constitute less than 1% of Greenland's area, contributed more than 12% of the net ice loss. This high-resolution record demonstrates that mass deficits extending over small spatial and temporal scales have made a relatively large contribution to recent ice sheet imbalance.

  16. In vivo high-resolution retinal imaging using adaptive optics.

    PubMed

    Seyedahmadi, Babak Jian; Vavvas, Demetrios

    2010-01-01

    Retinal imaging with conventional methods is only able to overcome the lowest order of aberration, defocus and astigmatism. The human eye is fraught with higher order of aberrations. Since we are forced to use the human optical system in retinal imaging, the images are degraded. In addition, all of these distortions are constantly changing due to head/eye movement and change in accommodation. Adaptive optics is a promising technology introduced in the field of ophthalmology to measure and compensate for these aberrations. High-resolution obtained by adaptive optics enables us to view and image the retinal photoreceptors, retina pigment epithelium, and identification of cone subclasses in vivo. In this review we will be discussing the basic technology of adaptive optics and hardware requirement in addition to clinical applications of such technology. PMID:21090998

  17. High-resolution extended source optical coherence tomography.

    PubMed

    Yu, Xiaojun; Liu, Xinyu; Chen, Si; Luo, Yuemei; Wang, Xianghong; Liu, Linbo

    2015-10-01

    High resolution optical coherence tomography (OCT) is capable of providing detailed tissue microstructures that are critical for disease diagnosis, yet its sensitivity is usually degraded since the system key components are typically not working at their respective center wavelengths. We developed a novel imaging system that achieves enhanced sensitivity without axial resolution degradation by the use of a spectrally encoded extended source (SEES) technique; it allows larger sample power without exceeding the maximum permissible exposure (MPE). In this study, we demonstrate a high-resolution extended source (HRES) OCT system, which is capable of providing a transverse resolution of 4.4 µm and an axial resolution of 2.1 µm in air with the SEES technique. We first theoretically show a sensitivity advantage of 6-dB of the HRES-OCT over that of its point source counterpart using numerical simulations, and then experimentally validate the applicability of the SEES technique to high-resolution OCT (HR-OCT) by comparing the HRES-OCT with an equivalent point-source system. In the HRES-OCT system, a dispersive prism was placed in the infinity space of the sample arm optics to spectrally extend the visual angle (angular subtense) of the light source to 10.3 mrad. This extended source allowed ~4 times larger MPE than its point source counterpart, which results in an enhancement of ~6 dB in sensitivity. Specifically, to solve the unbalanced dispersion between the sample and the reference arm optics, we proposed easy and efficient methods for system calibration and dispersion correction, respectively. With a maximum scanning speed reaching up to 60K A-lines/s, we further conducted imaging experiments with HRES-OCT using the human fingertip in vivo and the swine eye tissues ex vivo. Results demonstrate that the HRES-OCT is able to achieve significantly larger penetration depth than its conventional point source OCT counterpart. PMID:26480153

  18. Optical Histology: High-Resolution Visualization of Tissue Microvasculature

    NASA Astrophysics Data System (ADS)

    Moy, Austin Jing-Ming

    Mammalian tissue requires the delivery of nutrients, growth factors, and the exchange of oxygen and carbon dioxide gases to maintain normal function. These elements are delivered by the blood, which travels through the connected network of blood vessels, known as the vascular system. The vascular system consists of large feeder blood vessels (arteries and veins) that are connected to the small blood vessels (arterioles and venules), which in turn are connected to the capillaries that are directly connected to the tissue and facilitate gas exchange and nutrient delivery. These small blood vessels and capillaries make up an intricate but organized network of blood vessels that exist in all mammalian tissues known as the microvasculature and are very important in maintaining the health and proper function of mammalian tissue. Due to the importance of the microvasculature in tissue survival, disruption of the microvasculature typically leads to tissue dysfunction and tissue death. The most prevalent method to study the microvasculature is visualization. Immunohistochemistry (IHC) is the gold-standard method to visualize tissue microvasculature. IHC is very well-suited for highly detailed interrogation of the tissue microvasculature at the cellular level but is unwieldy and impractical for wide-field visualization of the tissue microvasculature. The objective my dissertation research was to develop a method to enable wide-field visualization of the microvasculature, while still retaining the high-resolution afforded by optical microscopy. My efforts led to the development of a technique dubbed "optical histology" that combines chemical and optical methods to enable high-resolution visualization of the microvasculature. The development of the technique first involved preliminary studies to quantify optical property changes in optically cleared tissues, followed by development and demonstration of the methodology. Using optical histology, I successfully obtained high

  19. All-Optical Ultrasound Transducers for High Resolution Imaging

    NASA Astrophysics Data System (ADS)

    Sheaff, Clay Smith

    High frequency ultrasound (HFUS) has increasingly been used within the past few decades to provide high resolution (< 200 mum) imaging in medical applications such as endoluminal imaging, intravascular imaging, ophthalmology, and dermatology. The optical detection and generation of HFUS using thin films offers numerous advantages over traditional piezoelectric technology. Circumvention of an electronic interface with the device head is one of the most significant given the RF noise, crosstalk, and reduced capacitance that encumbers small-scale electronic transducers. Thin film Fabry-Perot interferometers - also known as etalons - are well suited for HFUS receivers on account of their high sensitivity, wide bandwidth, and ease of fabrication. In addition, thin films can be used to generate HFUS when irradiated with optical pulses - a method referred to as Thermoelastic Ultrasound Generation (TUG). By integrating a polyimide (PI) film for TUG into an etalon receiver, we have created for the first time an all-optical ultrasound transducer that is both thermally stable and capable of forming fully sampled 2-D imaging arrays of arbitrary configuration. Here we report (1) the design and fabrication of PI-etalon transducers; (2) an evaluation of their optical and acoustic performance parameters; (3) the ability to conduct high-resolution imaging with synthetic 2-D arrays of PI-etalon elements; and (4) work towards a fiber optic PI-etalon for in vivo use. Successful development of a fiber optic imager would provide a unique field-of-view thereby exposing an abundance of prospects for minimally-invasive analysis, diagnosis, and treatment of disease.

  20. Next generation high resolution adaptive optics fundus imager

    NASA Astrophysics Data System (ADS)

    Fournier, P.; Erry, G. R. G.; Otten, L. J.; Larichev, A.; Irochnikov, N.

    2005-12-01

    The spatial resolution of retinal images is limited by the presence of static and time-varying aberrations present within the eye. An updated High Resolution Adaptive Optics Fundus Imager (HRAOFI) has been built based on the development from the first prototype unit. This entirely new unit was designed and fabricated to increase opto-mechanical integration and ease-of-use through a new user interface. Improved camera systems for the Shack-Hartmann sensor and for the scene image were implemented to enhance the image quality and the frequency of the Adaptive Optics (AO) control loop. An optimized illumination system that uses specific wavelength bands was applied to increase the specificity of the images. Sample images of clinical trials of retinas, taken with and without the system, are shown. Data on the performance of this system will be presented, demonstrating the ability to calculate near diffraction-limited images.

  1. ASIC-enabled High Resolution Optical Time Domain Reflectometer

    NASA Astrophysics Data System (ADS)

    Skendzic, Sandra

    Fiber optics has become the preferred technology in communication systems because of what it has to offer: high data transmission rates, immunity to electromagnetic interference, and lightweight, flexible cables. An optical time domain reflectometer (OTDR) provides a convenient method of locating and diagnosing faults (e.g. break in a fiber) along a fiber that can obstruct crucial optical pathways. Both the ability to resolve the precise location of the fault and distinguish between two discrete, closely spaced faults are figures of merit. This thesis presents an implementation of a high resolution OTDR through the use of a compact and programmable ASIC (application specific integrated circuit). The integration of many essential OTDR functions on a single chip is advantageous over existing commercial instruments because it enables small, lightweight packaging, and offers low power and cost efficiency. Furthermore, its compactness presents the option of placing multiple ASICs in parallel, which can conceivably ease the characterization of densely populated fiber optic networks. The OTDR ASIC consists of a tunable clock, pattern generator, precise timer, electrical receiver, and signal sampling circuit. During OTDR operation, the chip generates narrow electrical pulse, which can then be converted to optical format when coupled with an external laser diode driver. The ASIC also works with an external photodetector to measure the timing and amplitude of optical reflections in a fiber. It has a 1 cm sampling resolution, which allows for a 2 cm spatial resolution. While this OTDR ASIC has been previously demonstrated for multimode fiber fault diagnostics, this thesis focuses on extending its functionality to single mode fiber. To validate this novel approach to OTDR, this thesis is divided into five chapters: (1) introduction, (2) implementation, (3), performance of ASIC-based OTDR, (4) exploration in optical pre-amplification with a semiconductor optical amplifier, and

  2. High-Resolution Optical Tweezers for Single-Molecule Manipulation

    PubMed Central

    Zhang, Xinming; Ma, Lu; Zhang, Yongli

    2013-01-01

    Forces hold everything together and determine its structure and dynamics. In particular, tiny forces of 1-100 piconewtons govern the structures and dynamics of biomacromolecules. These forces enable folding, assembly, conformational fluctuations, or directional movements of biomacromolecules over sub-nanometer to micron distances. Optical tweezers have become a revolutionary tool to probe the forces, structures, and dynamics associated with biomacromolecules at a single-molecule level with unprecedented resolution. In this review, we introduce the basic principles of optical tweezers and their latest applications in studies of protein folding and molecular motors. We describe the folding dynamics of two strong coiled coil proteins, the GCN4-derived protein pIL and the SNARE complex. Both complexes show multiple folding intermediates and pathways. ATP-dependent chromatin remodeling complexes translocate DNA to remodel chromatin structures. The detailed DNA translocation properties of such molecular motors have recently been characterized by optical tweezers, which are reviewed here. Finally, several future developments and applications of optical tweezers are discussed. These past and future applications demonstrate the unique advantages of high-resolution optical tweezers in quantitatively characterizing complex multi-scale dynamics of biomacromolecules. PMID:24058311

  3. High Resolution Optical and NIR Spectra of HBC 722

    NASA Astrophysics Data System (ADS)

    Lee, Jeong-Eun; Park, Sunkyung; Green, Joel D.; Cochran, William D.; Kang, Wonseok; Lee, Sang-Gak; Sung, Hyun-Il

    2015-07-01

    We present the results of high resolution (R ≥ 30,000) optical and near-IR spectroscopic monitoring observations of HBC 722, a recent FU Orionis object that underwent an accretion burst in 2010. We observed HBC 722 in the optical/near-IR with the Bohyunsan Optical Echelle Spectrograph, Hobby-Eberly Telescope-HRS, and Immersion Grating Infrared Spectrograph, at various points in the outburst. We found atomic lines with strongly blueshifted absorption features or P Cygni profiles, both evidence of a wind driven by the accretion. Some lines show a broad double-peaked absorption feature, evidence of disk rotation. However, the wind-driven and disk-driven spectroscopic features are anti-correlated in time; the disk features became strong as the wind features disappeared. This anti-correlation might indicate that the rebuilding of the inner disk was interrupted by the wind pressure during the first 2 years. The half-width at half-depth of the double-peaked profiles decreases with wavelength, indicative of the Keplerian rotation; the optical spectra with the disk feature are fitted by a G5 template stellar spectrum convolved with a rotation velocity of 70 km s-1 while the near-IR disk features are fitted by a K5 template stellar spectrum convolved with a rotation velocity of 50 km s-1. Therefore, the optical and near-IR spectra seem to trace the disk at 39 and 76 R⊙, respectively. We fit a power-law temperature distribution in the disk, finding an index of 0.8, comparable to optically thick accretion disk models. Based on observations obtained with the Hobby-Eberly Telescope, which is a joint project of the University of Texas at Austin, the Pennsylvania State University, Stanford University, Ludwig-Maximilians-Universität München, and Georg-August-Universität Göttingen.

  4. High Resolution Optical and NIR Spectra of HBC 722

    NASA Astrophysics Data System (ADS)

    Lee, Jeong-Eun; Park, Sunkyung; Green, Joel D.; Cochran, William D.; Kang, Wonseok; Lee, Sang-Gak; Sung, Hyun-Il

    2015-07-01

    We present the results of high resolution (R ≥ 30,000) optical and near-IR spectroscopic monitoring observations of HBC 722, a recent FU Orionis object that underwent an accretion burst in 2010. We observed HBC 722 in the optical/near-IR with the Bohyunsan Optical Echelle Spectrograph, Hobby–Eberly Telescope-HRS, and Immersion Grating Infrared Spectrograph, at various points in the outburst. We found atomic lines with strongly blueshifted absorption features or P Cygni profiles, both evidence of a wind driven by the accretion. Some lines show a broad double-peaked absorption feature, evidence of disk rotation. However, the wind-driven and disk-driven spectroscopic features are anti-correlated in time; the disk features became strong as the wind features disappeared. This anti-correlation might indicate that the rebuilding of the inner disk was interrupted by the wind pressure during the first 2 years. The half-width at half-depth of the double-peaked profiles decreases with wavelength, indicative of the Keplerian rotation; the optical spectra with the disk feature are fitted by a G5 template stellar spectrum convolved with a rotation velocity of 70 km s‑1 while the near-IR disk features are fitted by a K5 template stellar spectrum convolved with a rotation velocity of 50 km s‑1. Therefore, the optical and near-IR spectra seem to trace the disk at 39 and 76 R⊙, respectively. We fit a power-law temperature distribution in the disk, finding an index of 0.8, comparable to optically thick accretion disk models. Based on observations obtained with the Hobby–Eberly Telescope, which is a joint project of the University of Texas at Austin, the Pennsylvania State University, Stanford University, Ludwig-Maximilians-Universität München, and Georg-August-Universität Göttingen.

  5. Urban Ecosystems from Spaceborne High-Resolution Optical Data

    NASA Astrophysics Data System (ADS)

    Haas, Jan; Jacob, Alexander; Ban, Yifang

    2014-11-01

    The potential of high-resolution optical satellite images for mapping of ecologically important urban space is investigated in this study. Both a GeoEye-1 and a Landsat 8 scene over central Shanghai were first segmented by two different algorithms and then classified into seven urban classes by SVM. Shadows in the pan-sharpened GeoEye-1 image were masked out and replaced by the corresponding pan-sharpened classified Landsat 8 image. Largest confusions occurred between sealed and permeable but non-vegetated surfaces, and between low-rise residential and high-rise commercial buildings. Based on the classification result, ecosystem service balances, supply and demand was modelled for each particular land cover class. Classification accuracies of 88% and 91% could be reached, indicating the suitability of the underlying data and method for this application domain. The KTH-SEG segmentation algorithm slightly outperformed the one implemented in eCognition. The highest supply of ecosystem services was found in water bodies whereas high-rise built-up areas revealed largest demands.

  6. Large Field, High Resolution Full-Field Optical Coherence Tomography

    PubMed Central

    Assayag, Osnath; Antoine, Martine; Sigal-Zafrani, Brigitte; Riben, Michael; Harms, Fabrice; Burcheri, Adriano; Grieve, Kate; Dalimier, Eugénie; Le Conte de Poly, Bertrand; Boccara, Claude

    2014-01-01

    We present a benchmark pilot study in which high-resolution Full-Field Optical Coherence Tomography (FF-OCT) was used to image human breast tissue and is evaluated to assess its ability to aid the pathologist’s management of intra-operative diagnoses. FF-OCT imaging safety was investigated and agreement between FF-OCT and routinely prepared histopathological images was evaluated. The compact setup used for this study provides 1 µm3 resolution and 200 µm imaging depth, and a 2.25 cm2 specimen is scanned in about 7 minutes. 75 breast specimens were imaged from 22 patients (21 women, 1 man) with a mean age of 58 (range: 25-83). Pathologists blind diagnosed normal/benign or malignant tissue based on FF-OCT images alone, diagnosis from histopathology followed for comparison. The contrast in the FF-OCT images is generated by intrinsic tissue scattering properties, meaning that no tissue staining or preparation is required. Major architectural features and tissue structures of benign breast tissue, including adipocytes, fibrous stroma, lobules and ducts were characterized. Subsequently, features resulting from pathological modification were characterized and a diagnosis decision tree was developed. Using FF-OCT images, two breast pathologists were able to distinguish normal/benign tissue from lesional with a sensitivity of 94% and 90%, and specificity of 75% and 79% respectively. PMID:24000981

  7. Holographic recording aspects of high-resolution Bayfol HX photopolymer

    NASA Astrophysics Data System (ADS)

    Berneth, Horst; Bruder, Friedrich K.; Fäcke, Thomas; Hagen, Rainer; Hönel, Dennis; Jurbergs, David; Rölle, Thomas; Weiser, Marc-Stephan

    2011-02-01

    We have been developing a new class of recording materials for volume holography, offering the advantages of full color recording and depth tuning without any chemical or thermal processing, combined with low shrinkage and detuning. These photopolymers are based on the two-chemistry concept in which the writing chemistry is dissolved in a preformed polymeric network. This network gives the necessary mechanical stability to the material prior to recording. In this paper we describe several aspects of holographic recording into Bayfol® HX which are beneficial for its effective use and discuss them within a more elaborate reaction-diffusion model. Inhibition phenomena and the influence of precure are studied within this model and are investigated experimentally for single hologram recording and angular multiplexed hologram recordings. Also the dark reaction after the exposure period and the minimum allowable waiting time for full hologram formation are addressed. The proper understanding of these phenomena is important for the optimal usage of these new materials, in for example step-and-repeat mass production of holograms.

  8. Special issue on high-resolution optical imaging

    NASA Astrophysics Data System (ADS)

    Smith, Peter J. S.; Davis, Ilan; Galbraith, Catherine G.; Stemmer, Andreas

    2013-09-01

    The pace of development in the field of advanced microscopy is truly breath-taking, and is leading to major breakthroughs in our understanding of molecular machines and cell function. This special issue of Journal of Optics draws attention to a number of interesting approaches, ranging from fluorescence and imaging of unlabelled cells, to computational methods, all of which are describing the ever increasing detail of the dynamic behaviour of molecules in the living cell. This is a field which traditionally, and currently, demonstrates a marvellous interplay between the disciplines of physics, chemistry and biology, where apparent boundaries to resolution dissolve and living cells are viewed in ever more clarity. It is fertile ground for those interested in optics and non-conventional imaging to contribute high-impact outputs in the fields of cell biology and biomedicine. The series of articles presented here has been selected to demonstrate this interdisciplinarity and to encourage all those with a background in the physical sciences to 'dip their toes' into the exciting and dynamic discoveries surrounding cell function. Although single molecule super-resolution microscopy is commercially available, specimen preparation and interpretation of single molecule data remain a major challenge for scientists wanting to adopt the techniques. The paper by Allen and Davidson [1] provides a much needed detailed introduction to the practical aspects of stochastic optical reconstruction microscopy, including sample preparation, image acquisition and image analysis, as well as a brief description of the different variants of single molecule localization microscopy. Since super-resolution microscopy is no longer restricted to three-dimensional imaging of fixed samples, the review by Fiolka [2] is a timely introduction to techniques that have been successfully applied to four-dimensional live cell super-resolution microscopy. The combination of multiple high-resolution techniques

  9. Special issue on high-resolution optical imaging

    NASA Astrophysics Data System (ADS)

    Smith, Peter J. S.; Davis, Ilan; Galbraith, Catherine G.; Stemmer, Andreas

    2013-09-01

    The pace of development in the field of advanced microscopy is truly breath-taking, and is leading to major breakthroughs in our understanding of molecular machines and cell function. This special issue of Journal of Optics draws attention to a number of interesting approaches, ranging from fluorescence and imaging of unlabelled cells, to computational methods, all of which are describing the ever increasing detail of the dynamic behaviour of molecules in the living cell. This is a field which traditionally, and currently, demonstrates a marvellous interplay between the disciplines of physics, chemistry and biology, where apparent boundaries to resolution dissolve and living cells are viewed in ever more clarity. It is fertile ground for those interested in optics and non-conventional imaging to contribute high-impact outputs in the fields of cell biology and biomedicine. The series of articles presented here has been selected to demonstrate this interdisciplinarity and to encourage all those with a background in the physical sciences to 'dip their toes' into the exciting and dynamic discoveries surrounding cell function. Although single molecule super-resolution microscopy is commercially available, specimen preparation and interpretation of single molecule data remain a major challenge for scientists wanting to adopt the techniques. The paper by Allen and Davidson [1] provides a much needed detailed introduction to the practical aspects of stochastic optical reconstruction microscopy, including sample preparation, image acquisition and image analysis, as well as a brief description of the different variants of single molecule localization microscopy. Since super-resolution microscopy is no longer restricted to three-dimensional imaging of fixed samples, the review by Fiolka [2] is a timely introduction to techniques that have been successfully applied to four-dimensional live cell super-resolution microscopy. The combination of multiple high-resolution techniques

  10. High resolution mesospheric sodium properties for adaptive optics applications

    NASA Astrophysics Data System (ADS)

    Pfrommer, T.; Hickson, P.

    2014-05-01

    Context. The performance of laser guide star adaptive optics (AO) systems for large optical and infrared telescopes is affected by variability of the sodium layer, located at altitudes between 80 and 120 km in the upper mesosphere and lower thermosphere. The abundance and density structure of the atomic sodium found in this region is subject to local and global weather effects, planetary and gravity waves and magnetic storms, and is variable on time scales down to tens of milliseconds, a range relevant to AO. Aims: It is therefore important to characterize the structure and dynamical evolution of the sodium region on small, as well as large spatial and temporal scales. Parameters of particular importance for AO are the mean sodium altitude, sodium layer width and the temporal power spectrum of the centroid altitude. Methods: We have conducted a three-year campaign employing a high-resolution lidar system installed on the 6-m Large Zenith Telescope (LZT) located near Vancouver, Canada. During this period, 112 nights of useful data were obtained. Results: The vertical density profile of atomic sodium shows remarkable structure and variability. Smooth Gaussian-shaped profiles rarely occur. Multiple internal layers are frequently found. These layers often have sharp lower edges, with scale heights of just a few hundred meters, and tend to drift downwards at a typical rate of one kilometer every two to three hours. Individual layers can persist for many hours, but their density and internal structure can be highly variable. Sporadic layers are seen reaching peak densities several times the average, often in just a few minutes. Coherent vertical oscillations are often found, typically extending over tens of kilometers in altitude. Regions of turbulence are evident and Kelvin-Helmholtz instability are sometimes seen. The mean value of the centroid altitude is found to be 90.8 ± 0.1 km. The sodium layer width was determined by computing the altitude range that contains a

  11. High resolution interferometer with multiple-pass optical configuration.

    PubMed

    Ahn, Jeongho; Kim, Jong-Ahn; Kang, Chu-Shik; Kim, Jae-Wan; Kim, Soohyun

    2009-11-01

    An interferometer having fourteen times higher resolution than a conventional single-pass interferometer has been developed by making multiple-pass optical path. To embody the multiple-pass optical configuration, a two-dimensional corner cube array block was designed, and its symmetric structure minimized the measurement error. The effect from the alignment error and the imperfection of corner cube is calculated as picometer level. An experiment proves that the suggested interferometer has about 45 nm of optical resolution and its nonlinearity is about 0.5 nm in peak-to-valley. PMID:19997342

  12. Adaptive Optics Technology for High-Resolution Retinal Imaging

    PubMed Central

    Lombardo, Marco; Serrao, Sebastiano; Devaney, Nicholas; Parravano, Mariacristina; Lombardo, Giuseppe

    2013-01-01

    Adaptive optics (AO) is a technology used to improve the performance of optical systems by reducing the effects of optical aberrations. The direct visualization of the photoreceptor cells, capillaries and nerve fiber bundles represents the major benefit of adding AO to retinal imaging. Adaptive optics is opening a new frontier for clinical research in ophthalmology, providing new information on the early pathological changes of the retinal microstructures in various retinal diseases. We have reviewed AO technology for retinal imaging, providing information on the core components of an AO retinal camera. The most commonly used wavefront sensing and correcting elements are discussed. Furthermore, we discuss current applications of AO imaging to a population of healthy adults and to the most frequent causes of blindness, including diabetic retinopathy, age-related macular degeneration and glaucoma. We conclude our work with a discussion on future clinical prospects for AO retinal imaging. PMID:23271600

  13. High energy, high resolution X-ray optics

    NASA Technical Reports Server (NTRS)

    Weisskopf, Martin C.; Joy, Marshall; Kahn, Steven

    1990-01-01

    The scientific goals of X-ray astronomy are considered to evaluate the relative advantages of using classical Wolter-1 optics or using a different approach. The portion of the X-ray band over 10 keV is unexploited in the present X-ray optics technology, and focussing in this portion of the band is crucial because nonfocussed experiments are background limited. The basic design of 'hard' X-ray optics is described theoretically emphasizing the very small angles of incidence in the grazing-incidence optics. Optimization of the signal-to-noise ratio is found to occur at a finite angular resolution. In real applications, the effective area reduced by the efficiency of the two reflections is 80 percent at energies up to 40 keV, and the quality of the reflecting surface can be monitored to minimize scattering. Focussing optics are found to offer improvements in signal-to-noise as well as more effective scientific return because microelectronic focal-plane technology is employed.

  14. High-resolution optical frequency metrology with stabilized femtosecond lasers

    NASA Astrophysics Data System (ADS)

    Jones, Ronald Jason

    The merging of such seemingly disparate fields as optical frequency metrology and ultrafast physics over the past few years has had a revolutionary impact on both fields. Extensive research over the past several decades has focused on stabilizing cw lasers to atomic and molecular transitions. These transitions in the optical and near-infrared regimes provide some of the highest Q's accessible in spectroscopy due to their high resonant frequencies (Q ≡ nu o/deltanu). Modern experiments have enjoyed increasing levels of precision and accuracy due to such stabilized laser systems. A long standing problem in optical frequency metrology, however, is the difficulty to perform direct frequency measurements in the optical spectrum. Traditional optical frequency chains are complex, costly, and lack flexibility. Recent experiments based on mode-locked femtosecond (fs) lasers promise to eliminate this problem and make optical frequency measurements accessible as a general laboratory tool. The use of fs lasers now enables the direct measurement of optical transitions by simply linking these frequencies to the repetition rate of the fs laser. The ability of the femtosecond laser to link the optical and radio frequency regimes is ultimately limited by its stability. In this dissertation, we present a novel stabilization scheme in which the frequency, phase, and repetition rate of a Kerr-lens mode-locked (KLM) ti:sapphire laser are locked to that of an ultra-stable Fabry-Perot reference cavity. The large signal to noise ratio of the recovered cavity resonance allows the superb short term stability (tau < 1 second) of the passive reference cavity to be transferred to the femtosecond laser. This technique may find future application in any experiment involving the use of femtosecond pulses in which a resonant cavity is employed, such as intracavity studies of light-matter interactions with ultra-short pulses. The short term instability of the cavity stabilized femtosecond laser

  15. Bendable X-ray Optics for High Resolution Imaging

    NASA Technical Reports Server (NTRS)

    Gubarev, M.; Ramsey, B.; Kilaru, K.; Atkins, C.; Broadway, D.

    2014-01-01

    Current state-of the-art for x-ray optics fabrication calls for either the polishing of massive substrates into high-angular-resolution mirrors or the replication of thin, lower-resolution, mirrors from perfectly figured mandrels. Future X-ray Missions will require a change in this optics fabrication paradigm in order to achieve sub-arcsecond resolution in light-weight optics. One possible approach to this is to start with perfectly flat, light-weight surface, bend it into a perfect cone, form the desired mirror figure by material deposition, and insert the resulting mirror into a telescope structure. Such an approach is currently being investigated at MSFC, and a status report will be presented detailing the results of finite element analyses, bending tests and differential deposition experiments.

  16. On a possibility high resolution optical tomograph design

    NASA Astrophysics Data System (ADS)

    Khohlov, K. L.; Sokolov, V. K.

    2006-04-01

    An actual problem of the modern medicine is statement of the diagnosis of diseases of a thyroid gland of the person. Extreme sensitivity of this organ to a X-ray radiation and invasive to methods puts a problem about development of an optical tomograph of a high solution. For this purpose the method of coherent optical detection (CDT) in a combination to a posteriori handling can be used with the purpose of elimination of influence of a spread function of a figuring system.

  17. High resolution underwater fiber optic threat detection system

    NASA Astrophysics Data System (ADS)

    Berger, Alexander; Hermesh, Shalmon; Durets, Eugene; Kempen, Lothar U.

    2006-10-01

    Current underwater protection systems are complex expensive devices consisting of multiple electronic sensing elements. The detection and identification of divers and small submerged watercraft requires very high image resolution. The high price of an array of conventional piezoelectric transducers and associated electronic components makes this solution feasible for localized implementations, but the protection of large stretches of coastline requires a different approach. We present a novel multichannel sonar design that augments current active sonar transducers with a passive fiber-optic multichannel acoustic emission sensing array. The system provides continuous monitoring of the acoustic wave reflections emitted by a single projector, yielding information about the size and shape of approaching objects. A novel fiber hydrophone enclosure is utilized to dramatically enhance the sensor response to the sonar frequency, while suppressing out-of-band sound sources and noise. The ability of a fiber hydrophone to respond to acoustic emissions is based on established fiber Bragg grating sensing techniques. In this approach, the energy of an acoustic wave is converted into the modulation of the in-fiber optical transducer's optical properties. The obtained results demonstrate significant response of the designed fiber optic hydrophone to the incident acoustic wave over the frequency domain from 1-80 kHz. Our approach allows selective tuning of the sensor to a particular acoustic frequency, as well as potential extension of the spectral response to 300- 400kHz.2

  18. Optical system for high resolution spectrometer/monochromator

    DOEpatents

    Hettrick, Michael C.; Underwood, James H.

    1988-01-01

    An optical system for use in a spectrometer or monochromator employing a mirror which reflects electromagnetic radiation from a source to converge with same in a plane. A straight grooved, varied-spaced diffraction grating receives the converging electromagnetic radiation from the mirror and produces a spectral image for capture by a detector, target or like receiver.

  19. High resolution optical calorimetry for synchrotron microbeam radiation therapy

    NASA Astrophysics Data System (ADS)

    Ackerly, T.; Crosbie, J. C.; Fouras, A.; Sheard, G. J.; Higgins, S.; Lewis, R. A.

    2011-03-01

    We propose the application of optical calorimetry to measure the peak to valley ratio for synchrotron microbeam radiation therapy (MRT). We use a modified Schlieren approach known as reference image topography (RIT) which enables one to obtain a map of the rate of change of the refractive index in a water bath from which the absorbed dose can be determined with sufficient spatial accuracy to determine the peak to valley ratio. We modelled the calorimetric properties of X-rays using a heated wire in a water bath. Our RIT system comprised a light source, a textured reference object and a camera and lens combination. We measured temperature contours and showed a plume rising from the heated wire. The total temperature change in water was 12 degrees C, 500 times greater than the calculated change from a 1 ms exposure on a synchrotron. At 1.0 ms, thermal diffusion will be the major cause of uncertainty in determining the peak to valley ratio, and we calculate thermal diffusion will reduce the measured peak to valley ratio to 76% of its initial value, but the individual microbeams will still resolve. We demonstrate proof of concept for measuring X-ray dose using a modified RIT method.

  20. High-resolution proxy record of Holocene climate from a loess section in Southwestern Nebraska, USA

    USGS Publications Warehouse

    Miao, X.; Mason, J.A.; Johnson, W.C.; Wang, Hongfang

    2007-01-01

    Multi-proxy analysis was used to produce a high-resolution paleoclimatic record from an exceptionally thick section of the Holocene Bignell Loess near Wauneta, Southwestern Nebraska, in the central Great Plains. The Wauneta section has excellent age control, based on optically stimulated luminescence (OSL) and radiocarbon dating, and records multiple episodes of rapid loess deposition alternating with slower deposition and soil formation. The lowermost and uppermost OSL ages obtained from the Bignell Loess are 10,250 ?? 610??years (5.9??m depth) and 100 ?? 10??years (0.1??m depth), respectively. As a result, the Holocene has been temporally confined. Stratigraphically, the Bignell Loess overlies the Late Pleistocene Peoria Loess (deposited ??? 21-14??ka), and the two units are separated by the Brady Soil which is distinguished by its color and other pedogenic features. L*a*b* color parameters and organic carbon content of Bignell Loess are sensitive proxies to differentiate drought-induced aeolian sediment layers from the intercalated soil horizons. Soil organic carbon-derived ??13C data suggest that the C3-dominated floral environment during Peoria Loess deposition shifted dramatically to a C4-dominated environment during Brady Soil formation in response to a warming trend. Even greater C4 abundance characterized the late Holocene. High-resolution ??13C data support the contention that C3 vs. C4 vegetation change in the Holocene reflects ecosystem response to frequent vegetation disturbance under arid conditions. Time series analysis reveals that ??13C and color parameters display high frequency variation with periodicities of 103-118??years and 103??years, respectively. Similar periodicities were also reported in studies of North Dakota lakes, though the physical mechanism responsible is uncertain. Comparison of Bignell Loess color and tropical Pacific sea surface temperatures (SSTs) allows evaluation of a proposed teleconnection between drought in the Great

  1. High-resolution fracture aperture mapping using optical profilometry

    NASA Astrophysics Data System (ADS)

    Ameli, Pasha; Elkhoury, Jean E.; Detwiler, Russell L.

    2013-10-01

    Fractures play an important role in the Earth's crust, often controlling both mechanical and transport processes. Developing a mechanistic understanding of these processes requires quantifying the roughness of fracture surfaces and the contacts and void spaces between fracture surfaces at high spatial resolution (10s of microns) over a broad range of scales (centimeters to meters). Here we present a scalable method for measuring fracture surfaces and reconstructing fracture aperture fields using an optical profilometer. We evaluate the method by measuring two fractured limestone cores; one is a tensile fracture with strong cross correlation between the surfaces and the other is a saw-cut, sand-blasted fracture with negligible cross correlation between the surfaces. Results of repeated measurements of these two fractures suggest that well-correlated surfaces, where the correlation between the surfaces can aid reconstruction, can be reproduced with local uncertainties with median standard deviation of 8 μm . Poorly correlated surfaces, where reconstruction relies solely upon the precision of the placement of the halves of the core on the profilometer stage, can be reproduced with local uncertainties with median standard deviation of 20 μm . Additionally, we quantified the accuracy of the technique by comparing calculated aperture profiles of a fractured concrete core to thin sections cut from the core after impregnating it with epoxy. The median deviation between the two measurements, which includes errors due to residual misalignment of the profiles, was 29 μm supporting the accuracy of the method. Our results emphasize the potential for using noncontact surface measurement techniques to accurately and precisely reconstruct fracture apertures over a wide range of length scales.

  2. Advanced high-resolution mask processes using optical proximity correction

    NASA Astrophysics Data System (ADS)

    Chan, Y. David

    1999-08-01

    The benefits of incorporating some 'distortion' to the design data in order to produce the desired results on the wafers has been recognized for many years. This 'distortion' has come to be commonly referred to as optical proximity correction (OPC) by the lithography community. In today's era of high throughput laser reticle writing tools, line shortening and corner rounding has forced OPC up the lithography tree from wafer imaging to reticle imaging. With the increasing popularity of 4X systems, the comparatively large spot laser reticle writing systems in the field today need to be extended before being rendered useless for critical reticle requirements due to reticle corner rounding, line shortening and scatter bar resolution. These problems must be resolved in order to extend the use of laser tool for technology node below 0.25 micrometer. Some previous work has been done in adding corner serifs to eliminate corner rounding in contact holes. It was clear from the results that the optimal serifs sizes could be different when patterns were written on different tools. However, there is no clear understanding how the process may affect the outcome. A recent paper by W. Ziegler, et al shows the effect of adding small serifs to line ends on line end shortening based on aerial image and wafer measurement. This paper will discuss the effect of Laser Proximity correction (LPC) and the reticle manufacturing processes on pattern fidelity. CAPROX LPCTM is used to correct for distoritons during the mask exposure. Not only will the impact of lithographic tools on OPC be discussed, but an examination of the effect of wet and dry etched processes on corner rounding, image fidelity, and line end shortening will also be presented.

  3. High resolution hybrid optical and acoustic sea floor maps (Invited)

    NASA Astrophysics Data System (ADS)

    Roman, C.; Inglis, G.

    2013-12-01

    This abstract presents a method for creating hybrid optical and acoustic sea floor reconstructions at centimeter scale grid resolutions with robotic vehicles. Multibeam sonar and stereo vision are two common sensing modalities with complementary strengths that are well suited for data fusion. We have recently developed an automated two stage pipeline to create such maps. The steps can be broken down as navigation refinement and map construction. During navigation refinement a graph-based optimization algorithm is used to align 3D point clouds created with both the multibeam sonar and stereo cameras. The process combats the typical growth in navigation error that has a detrimental affect on map fidelity and typically introduces artifacts at small grid sizes. During this process we are able to automatically register local point clouds created by each sensor to themselves and to each other where they overlap in a survey pattern. The process also estimates the sensor offsets, such as heading, pitch and roll, that describe how each sensor is mounted to the vehicle. The end results of the navigation step is a refined vehicle trajectory that ensures the points clouds from each sensor are consistently aligned, and the individual sensor offsets. In the mapping step, grid cells in the map are selectively populated by choosing data points from each sensor in an automated manner. The selection process is designed to pick points that preserve the best characteristics of each sensor and honor some specific map quality criteria to reduce outliers and ghosting. In general, the algorithm selects dense 3D stereo points in areas of high texture and point density. In areas where the stereo vision is poor, such as in a scene with low contrast or texture, multibeam sonar points are inserted in the map. This process is automated and results in a hybrid map populated with data from both sensors. Additional cross modality checks are made to reject outliers in a robust manner. The final

  4. High resolution light intensity spectrum analyzer (LISA) based on Brillouin optical filter

    NASA Astrophysics Data System (ADS)

    Heras, C.; Subías, J.; Pelayo, J.; Villuendas, F.

    2007-04-01

    A high-resolution light intensity spectrum analyzer technique to derive the RF modulation spectrum of optical signals is presented and experimentally confirmed. It uses the XPM nonlinear effect in a dispersion shifted fiber to obtain the light intensity spectrum, and a Brillouin optical filtering method to implement the high resolution spectrometric analysis. Measured RF spectra of PRBS modulated optical signals at 2.5 Gb/s and 10 Gb/s are presented and compared with their corresponding ones obtained in the electrical detection domain to confirm the capabilities of the method.Influence of fiber electrostriction effect is measured and analyzed.

  5. High resolution optical surface metrology with the slope measuring portable optical test system

    NASA Astrophysics Data System (ADS)

    Maldonado, Alejandro V.

    New optical designs strive to achieve extreme performance, and continually increase the complexity of prescribed optical shapes, which often require wide dynamic range and high resolution. SCOTS, or the Software Configurable Optical Test System, can measure a wide range of optical surfaces with high sensitivity using surface slope. This dissertation introduces a high resolution version of SCOTS called SPOTS, or the Slope measuring Portable Optical Test System. SPOTS improves the metrology of surface features on the order of sub-millimeter to decimeter spatial scales and nanometer to micrometer level height scales. Currently there is no optical surface metrology instrument with the same utility. SCOTS uses a computer controlled display (such as an LCD monitor) and camera to measure surface slopes over the entire surface of a mirror. SPOTS differs in that an additional lens is placed near the surface under test. A small prototype system is discussed in general, providing the support for the design of future SPOTS devices. Then the SCOTS instrument transfer function is addressed, which defines the way the system filters surface heights. Lastly, the calibration and performance of larger SPOTS device is analyzed with example measurements of the 8.4-m diameter aspheric Large Synoptic Survey Telescope's (LSST) primary mirror. In general optical systems have a transfer function, which filters data. In the case of optical imaging systems the instrument transfer function (ITF) follows the modulation transfer function (MTF), which causes a reduction of contrast as a function of increasing spatial frequency due to diffraction. In SCOTS, ITF is shown to decrease the measured height of surface features as their spatial frequency increases, and thus the SCOTS and SPOTS ITF is proportional to their camera system's MTF. Theory and simulations are supported by a SCOTS measurement of a test piece with a set of lithographically written sinusoidal surface topographies. In addition, an

  6. Acoustic force mapping in a hybrid acoustic-optical micromanipulation device supporting high resolution optical imaging.

    PubMed

    Thalhammer, Gregor; McDougall, Craig; MacDonald, Michael Peter; Ritsch-Marte, Monika

    2016-04-12

    Many applications in the life-sciences demand non-contact manipulation tools for forceful but nevertheless delicate handling of various types of sample. Moreover, the system should support high-resolution optical imaging. Here we present a hybrid acoustic/optical manipulation system which utilizes a transparent transducer, making it compatible with high-NA imaging in a microfluidic environment. The powerful acoustic trapping within a layered resonator, which is suitable for highly parallel particle handling, is complemented by the flexibility and selectivity of holographic optical tweezers, with the specimens being under high quality optical monitoring at all times. The dual acoustic/optical nature of the system lends itself to optically measure the exact acoustic force map, by means of direct force measurements on an optically trapped particle. For applications with (ultra-)high demand on the precision of the force measurements, the position of the objective used for the high-NA imaging may have significant influence on the acoustic force map in the probe chamber. We have characterized this influence experimentally and the findings were confirmed by model simulations. We show that it is possible to design the chamber and to choose the operating point in such a way as to avoid perturbations due to the objective lens. Moreover, we found that measuring the electrical impedance of the transducer provides an easy indicator for the acoustic resonances. PMID:27025398

  7. High-resolution handheld rigid endomicroscope based on full-field optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Benoit a la Guillaume, Emilie; Martins, Franck; Boccara, Claude; Harms, Fabrice

    2016-02-01

    Full-field optical coherence tomography (FF-OCT) is a powerful tool for nondestructive assessment of biological tissue, i.e., for the structural examination of tissue in depth at a cellular resolution. Mostly known as a microscopy device for ex vivo analysis, FF-OCT has also been adapted to endoscopy setups since it shows good potential for in situ cancer diagnosis and biopsy guidance. Nevertheless, all the attempts to perform endoscopic FF-OCT imaging did not go beyond lab setups. We describe here, to the best of our knowledge, the first handheld FF-OCT endoscope based on a tandem interferometry assembly using incoherent illumination. A common-path passive imaging interferometer at the tip of an optical probe makes it robust and insensitive to environmental perturbations, and a low finesse Fabry-Perot processing interferometer guarantees a compact system. A good resolution (2.7 μm transverse and 6 μm axial) is maintained through the long distance, small diameter relay optics of the probe, and a good signal-to-noise ratio is achieved in a limited 100 ms acquisition time. High-resolution images and a movie of a rat brain slice have been recorded by moving the contact endoscope over the surface of the sample, allowing for tissue microscopic exploration at 20 μm under the surface. These promising ex vivo results open new perspectives for in vivo imaging of biological tissue, in particular, in the field of cancer and surgical margin assessment.

  8. High-resolution handheld rigid endomicroscope based on full-field optical coherence tomography.

    PubMed

    Benoit a la Guillaume, Emilie; Martins, Franck; Boccara, Claude; Harms, Fabrice

    2016-02-01

    Full-field optical coherence tomography (FF-OCT) is a powerful tool for nondestructive assessment of biological tissue, i.e., for the structural examination of tissue in depth at a cellular resolution. Mostly known as a microscopy device for ex vivo analysis, FF-OCT has also been adapted to endoscopy setups since it shows good potential for in situ cancer diagnosis and biopsy guidance. Nevertheless, all the attempts to perform endoscopic FF-OCT imaging did not go beyond lab setups. We describe here, to the best of our knowledge, the first handheld FF-OCT endoscope based on a tandem interferometry assembly using incoherent illumination. A common-path passive imaging interferometer at the tip of an optical probe makes it robust and insensitive to environmental perturbations, and a low finesse Fabry-Perot processing interferometer guarantees a compact system. A good resolution (2.7 μm transverse and 6 μm axial) is maintained through the long distance, small diameter relay optics of the probe, and a good signal-to-noise ratio is achieved in a limited 100 ms acquisition time. High-resolution images and a movie of a rat brain slice have been recorded by moving the contact endoscope over the surface of the sample, allowing for tissue microscopic exploration at 20 m under the surface. These promising ex vivo results open new perspectives for in vivo imaging of biological tissue, in particular, in the field of cancer and surgical margin assessment. PMID:26857471

  9. High-Resolution Holocene Records of Paleoceanographic and Paleoclimatic Variability from the Southern Alaskan Continental Margin

    NASA Astrophysics Data System (ADS)

    Finney, B. P.; Jaeger, J. M.; Mix, A. C.; Cowan, E. A.; Gulick, S. S.; Mayer, L. A.; Pisias, N. G.; Powell, R. D.; Prahl, F.; Stoner, J. S.

    2004-12-01

    We are investigating sediments from the fjords and continental margin of southern Alaska to develop high-resolution climatic and oceanographic records for the Late Quaternary. Our goal is to better understand linkages between climatic, terrestrial and oceanic systems in this tectonically active and biologically productive region. A field program was conducted aboard the R/V Maurice Ewing in August/September 2004 utilizing geophysical surveys (high-resolution swath bathymetric and backscatter imaging, shallow sub-bottom profiling, and where permitted, high-resolution seismic reflection profiling), piston and multi-coring, and CTD/water sampling at about 30 sites in this region. Cores are being analyzed for sedimentological, microfossil, geochemical and stable isotopic proxies, with chronologies constrained by Pb-210, AMS radiocarbon, tephrochronolgic and paleomagnetic dating. Our preliminary results demonstrate that these rapidly accumulating sedimentary archives can resolve environmental changes on annual to decadal timescales. Records of recent changes in lithogenic sediment accumulation and biological productivity on the Gulf of Alaska shelf track historical climatic data that extends to the early 20th century in this region. The records also correlate with multi-decadal climate regimes during the Little Ice Age as suggested by tree-ring, glacial advance and salmon abundance records from nearby coastal sites. Jack Dymond's enthusiasm for collaborative, interdisciplinary research will help guide us in unraveling the fingerprints of key processes in this relatively unexplored region.

  10. Computational high-resolution optical imaging of the living human retina

    NASA Astrophysics Data System (ADS)

    Shemonski, Nathan D.; South, Fredrick A.; Liu, Yuan-Zhi; Adie, Steven G.; Scott Carney, P.; Boppart, Stephen A.

    2015-07-01

    High-resolution in vivo imaging is of great importance for the fields of biology and medicine. The introduction of hardware-based adaptive optics (HAO) has pushed the limits of optical imaging, enabling high-resolution near diffraction-limited imaging of previously unresolvable structures. In ophthalmology, when combined with optical coherence tomography, HAO has enabled a detailed three-dimensional visualization of photoreceptor distributions and individual nerve fibre bundles in the living human retina. However, the introduction of HAO hardware and supporting software adds considerable complexity and cost to an imaging system, limiting the number of researchers and medical professionals who could benefit from the technology. Here we demonstrate a fully automated computational approach that enables high-resolution in vivo ophthalmic imaging without the need for HAO. The results demonstrate that computational methods in coherent microscopy are applicable in highly dynamic living systems.

  11. Computational high-resolution optical imaging of the living human retina

    PubMed Central

    Shemonski, Nathan D.; South, Fredrick A.; Liu, Yuan-Zhi; Adie, Steven G.; Carney, P. Scott; Boppart, Stephen A.

    2015-01-01

    High-resolution in vivo imaging is of great importance for the fields of biology and medicine. The introduction of hardware-based adaptive optics (HAO) has pushed the limits of optical imaging, enabling high-resolution near diffraction-limited imaging of previously unresolvable structures1,2. In ophthalmology, when combined with optical coherence tomography, HAO has enabled a detailed three-dimensional visualization of photoreceptor distributions3,4 and individual nerve fibre bundles5 in the living human retina. However, the introduction of HAO hardware and supporting software adds considerable complexity and cost to an imaging system, limiting the number of researchers and medical professionals who could benefit from the technology. Here we demonstrate a fully automated computational approach that enables high-resolution in vivo ophthalmic imaging without the need for HAO. The results demonstrate that computational methods in coherent microscopy are applicable in highly dynamic living systems. PMID:26877761

  12. Observation of thermomagnetically recorded domains with high-resolution magnetic soft x-ray microscopy

    NASA Astrophysics Data System (ADS)

    Tsunashima, S.; Takagi, Nayuki; Yamaguchi, Atushi; Kume, Minoru; Fischer, P.; Kumazawa, M.

    2003-04-01

    Magnetic domains were thermomagnetically recorded on TbFeCo films using laser pulsed magnetic field modulation (LP-MFM) and light intensity modulation (LIM). The domains were observed with high resolution magnetic transmission X-ray microscopy (M-TXM) before and after the heat treatment in order to clarify the recording characteristics and the thermal stability of recorded domains. From the results of M-TXM images, it was found that isolated single marks whose lengths are much smaller than 100 nm can be recorded by LP-MFM but their mark lengths become often longer than designed. It was further confirmed that the heat treatment at 120 degree C for 50 hours does not influence significantly the crescent-shaped magnetic domains of 100 nm in width recorded using LP-MFM method and circular domains of 150 nm in diameter recorded using LIM method.

  13. High resolution 900 yr volcanic and climatic record from the Vostok area, East Antarctica

    NASA Astrophysics Data System (ADS)

    Osipov, E. Yu.; Khodzher, T. V.; Golobokova, L. P.; Onischuk, N. A.; Lipenkov, V. Ya.; Ekaykin, A. A.; Osipova, O. P.

    2013-05-01

    Detailed volcanic record of the last 900 yr (1093-2010 AD) has been received using high resolution (2-3 samples per accumulation year) sulfate measurements in four snow/firn cores from the Vostok station area, East Antarctica. Totally, 33 volcanic events have been identified in the record, including well-known low latitude eruption signals found in many polar ice cores (e.g., Pinatubo 1991, Agung 1963, Krakatoa 1883, Tambora 1815, Huanaputina 1600, Kuwae 1452), however in comparison with other Antarctic sites the record has more events covering the last 900 yr. The strongest volcanic signals occurred during mid-13th, mid-15th and 18th centuries. The largest volcanic signal of Vostok (both in sulfate concentration and flux) is the 1452 AD Kuwae eruption. Average snow accumulation rate calculated for the period 1093-2010 AD is 21.3 ± 2.3 mm H2O. Accumulation record demonstrates a slight positive trend, however sharply increased accumulation rate during the periods from 1600 to 1815 AD (by 11% from long-term mean) and from 1963 to 2010 AD (by 15%) are typical features of the site. Na+ record shows strong decadal-scale variability probably connected with coupled changes in atmospheric transport patterns over Antarctica (meridional circulation change) and local glaciology. The obtained high resolution climatic records suggest a high sensitivity of the Vostok location to environmental changes in Southern Hemisphere.

  14. High-resolution visualization of mouse cardiac microvasculature using optical histology

    PubMed Central

    Moy, Austin J.; Lo, Patrick C.; Choi, Bernard

    2013-01-01

    Cardiovascular disease typically is associated with dysfunction of the coronary vasculature and microvasculature. The study of cardiovascular disease typically involves imaging of the large coronary vessels and quantification of cardiac blood perfusion. These methods, however, are not well suited for imaging of the cardiac microvasculature. We used the optical histology method, which combines chemical optical clearing and optical imaging, to create high-resolution, wide-field maps of the cardiac microvasculature in ventral slices of mouse heart. We have demonstrated the ability of the optical histology method to enable wide-field visualization of the cardiac microvasculature in high-resolution and anticipate that optical histology may have significant impact in studying cardiovascular disease. PMID:24466477

  15. A high-resolution record of atmospheric 14C based on Hulu Cave speleothem H82

    NASA Astrophysics Data System (ADS)

    Southon, John; Noronha, Alexandra L.; Cheng, Hai; Edwards, R. Lawrence; Wang, Yongjin

    2012-02-01

    The development of a calibration of atmospheric radiocarbon (∆14C) is a significant scientific goal because it provides the means to link the numerous 14C dated paleoclimate records to a common timescale with absolutely dated records, and thereby improve our understanding the relationships between the carbon cycle and climate change. Currently, few calibration datasets that directly sample the atmospheric 14C reservoir are available beyond the end of the dendro-dated Holocene tree ring record at 12.6 kyr BP (Before 1950 AD). In the absence of suitable true atmospheric records, 14C calibrations beyond this age limit are based largely on marine data, that are complicated by the marine reservoir effect, which may have varied over the glacial cycle. In this paper, we present a high-resolution record of U-Th series and 14C measurements from Hulu Cave speleothem H82, spanning 10.6-26.8 kyr BP. Corrections for detrital 230Th are negligible, and the contribution of 14C-free geologic carbon to the speleothem calcite is small (5-6%) and is stable across major climate shifts. The time series provides a 16 kyr record of atmospheric ∆14C as well as an updated age model for the existing Hulu Cave δ18O record. The 14C data are in good overall agreement with existing marine and terrestrial 14C records, but comparisons with the Cariaco Basin marine ∆14C record through the deglacial interval reveal that the Cariaco reservoir age appears to have varied during parts of the Younger Dryas and Heinrich Stadial 1 cold events. This highlights the importance of developing extended high-resolution marine and terrestrial 14C records as a means of detecting changes in ocean circulation over the glacial cycle.

  16. Optic for an endoscope/borescope having high resolution and narrow field of view

    DOEpatents

    Stone, Gary F.; Trebes, James E.

    2003-10-28

    An optic having optimized high spatial resolution, minimal nonlinear magnification distortion while at the same time having a limited chromatic focal shift or chromatic aberrations. The optic located at the distal end of an endoscopic inspection tool permits a high resolution, narrow field of view image for medical diagnostic applications, compared to conventional optics for endoscopic instruments which provide a wide field of view, low resolution image. The image coverage is over a narrow (<20 degrees) field of view with very low optical distortion (<5% pin cushion or barrel distortion. The optic is also optimized for best color correction as well as to aid medical diagnostics.

  17. High resolution atomic coherent control via spectral phase manipulation of an optical frequency comb.

    PubMed

    Stowe, Matthew C; Cruz, Flavio C; Marian, Adela; Ye, Jun

    2006-04-21

    We demonstrate high resolution coherent control of cold atomic rubidium utilizing spectral phase manipulation of a femtosecond optical frequency comb. Transient coherent accumulation is directly manifested by the enhancement of signal amplitude and spectral resolution via the pulse number. The combination of frequency comb technology and spectral phase manipulation enables coherent control techniques to enter a new regime with natural linewidth resolution. PMID:16712153

  18. High Resolution Atomic Coherent Control via Spectral Phase Manipulation of an Optical Frequency Comb

    SciTech Connect

    Stowe, Matthew C.; Cruz, Flavio C.; Marian, Adela; Ye Jun

    2006-04-21

    We demonstrate high resolution coherent control of cold atomic rubidium utilizing spectral phase manipulation of a femtosecond optical frequency comb. Transient coherent accumulation is directly manifested by the enhancement of signal amplitude and spectral resolution via the pulse number. The combination of frequency comb technology and spectral phase manipulation enables coherent control techniques to enter a new regime with natural linewidth resolution.

  19. Flexible high-resolution film recorder system. [in NASA image processing facility for remote sensor data

    NASA Technical Reports Server (NTRS)

    Heffner, P.; Connell, E.

    1980-01-01

    The paper describes a high-resolution film recorder (HRFR) system capable of meeting the requirements of all of the imaging sensors for the recording support of NASA missions. The technical requirements imposed by sensor constraints and end users of the film product are examined, along with the implementation techniques to satisfy these requirements. The recorder can produce annotated imagery with array sizes ranging from 1 to 400 million picture elements and a programmable radiometric transfer function provided by the recorder. The HRFR requirements were grouped into three categories: (1) front end (input) requirements defined by the input medium, (2) operational requirements based on the volume, throughput, and changeover time from one mode to another, and (3) film product requirements determined by the needs of the end product user.

  20. All-optical control of cardiac excitation: combined high-resolution optogenetic actuation and optical mapping.

    PubMed

    Entcheva, Emilia; Bub, Gil

    2016-05-01

    Cardiac tissue is an excitable system that can support complex spatiotemporal dynamics, including instabilities (arrhythmias) with lethal consequences. While over the last two decades optical mapping of excitation (voltage and calcium dynamics) has facilitated the detailed characterization of such arrhythmia events, until recently, no precise tools existed to actively interrogate cardiac dynamics in space and time. In this work, we discuss the combined use of new methods for space- and time-resolved optogenetic actuation and simultaneous fast, high resolution optical imaging of cardiac excitation waves. First, the mechanisms, limitations and unique features of optically induced responses in cardiomyocytes are outlined. These include the ability to bidirectionally control the membrane potential using depolarizing and hyperpolarizing opsins; the ability to induce prolonged sustained voltage changes; and the ability to control refractoriness and the shape of the cardiac action potential. At the syncytial tissue level, we discuss optogenetically enabled experimentation on cell-cell coupling, alteration of conduction properties and termination of propagating waves by light. Specific attention is given to space- and time-resolved application of optical stimulation using dynamic light patterns to perturb ongoing activation and to probe electrophysiological properties at desired tissue locations. The combined use of optical methods to perturb and to observe the system can offer new tools for precise feedback control of cardiac electrical activity, not available previously with pharmacological and electrical stimulation. These new experimental tools for all-optical electrophysiology allow for a level of precise manipulation and quantification of cardiac dynamics comparable in robustness to the computational setting, and can provide new insights into pacemaking, arrhythmogenesis and suppression or cardioversion. PMID:26857427

  1. High-Resolution Adaptive Optics Test-Bed for Vision Science

    SciTech Connect

    Wilks, S C; Thomspon, C A; Olivier, S S; Bauman, B J; Barnes, T; Werner, J S

    2001-09-27

    We discuss the design and implementation of a low-cost, high-resolution adaptive optics test-bed for vision research. It is well known that high-order aberrations in the human eye reduce optical resolution and limit visual acuity. However, the effects of aberration-free eyesight on vision are only now beginning to be studied using adaptive optics to sense and correct the aberrations in the eye. We are developing a high-resolution adaptive optics system for this purpose using a Hamamatsu Parallel Aligned Nematic Liquid Crystal Spatial Light Modulator. Phase-wrapping is used to extend the effective stroke of the device, and the wavefront sensing and wavefront correction are done at different wavelengths. Issues associated with these techniques will be discussed.

  2. High-resolution imaging of biological tissue with full-field optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Zhu, Yue; Gao, Wanrong

    2015-03-01

    A new full-field optical coherence tomography system with high-resolution has been developed for imaging of cells and tissues. Compared with other FF-OCT (Full-field optical coherence tomography, FF-OCT) systems illuminated with optical fiber bundle, the improved Köhler illumination arrangement with a halogen lamp was used in the proposed FF-OCT system. High numerical aperture microscopic objectives were used for imaging and a piezoelectric ceramic transducer (PZT) was used for phase-shifting. En-face tomographic images can be obtained by applying the five-step phase-shifting algorithm to a series of interferometric images which are recorded by a smart camera. Three-dimensional images can be generated from these tomographic images. Imaging of the chip of Intel Pentium 4 processor demonstrated the ultrahigh resolution of the system (lateral resolution is 0.8μm ), which approaches the theoretical resolution 0.7 μm× 0.5 μm (lateral × axial). En-face images of cells of onion show an excellent performance of the system in generating en-face images of biological tissues. Then, unstained pig stomach was imaged as a tissue and gastric pits could be easily recognized using FF-OCT system. Our study provides evidence for the potential ability of FFOCT in identifying gastric pits from pig stomach tissue. Finally, label-free and unstained ex vivo human liver tissues from both normal and tumor were imaged with this FFOCT system. The results show that the setup has the potential for medical diagnosis applications such liver cancer diagnosis.

  3. [Measurement of OH radicals in flame with high resolution differential optical absorption spectroscopy].

    PubMed

    Liu, Yu; Liu, Wen-Qing; Kan, Rui-Feng; Si, Fu-Qi; Xu, Zhen-Yu; Hu, Ren-Zhi; Xie, Pin-Hua

    2011-10-01

    The present paper describes a new developed high resolution differential optical absorption spectroscopy instrument used for the measurement of OH radicals in flame. The instrument consists of a Xenon lamp for light source; a double pass high resolution echelle spectrometer with a resolution of 3.3 pm; a multiple-reflection cell of 20 meter base length, in which the light reflects in the cell for 176 times, so the whole path length of light can achieve 3 520 meters. The OH radicals'6 absorption lines around 308 nm were simultaneously observed in the experiment. By using high resolution DOAS technology, the OH radicals in candles, kerosene lamp, and alcohol burner flames were monitored, and their concentrations were also inverted. PMID:22250529

  4. A high-resolution integrated optical spectrometer with applications to fibre sensor signal processing

    NASA Astrophysics Data System (ADS)

    Varasi, M.; Signorazzi, M.; Vannucci, A.; Dunphy, Jim

    1996-02-01

    An integrated optical device has been developed to realize the instrumentation for the processing of the optical signal such as those from fibre optic Bragg grating sensors (FBG) embedded in composite materials. The optical circuit integrates on X-cut 0957-0233/7/2/009/img7 an acousto-optical TE - TM converter included between two crossed polarizers, in order to realize a tunable high-resolution optical filter. The design and fabrication process parameters and solutions adopted with the aim of obtaining very-high-resolution filters ( FWHM < 0.45 nm at 1300 nm) are discussed. The device has been demonstrated to allow the polarization-independent spectrum analysis of in-fibre optical radiation, exploiting the heterodyne detection of the optical signals from the output of the acousto-optical tunable filter. The application as instrumentation for the processing of optical signals from such sensors is described and the experimental results are presented for the monitoring of static and dynamic deformations of composite material structures such as those in which the FBG sensors have been embedded.

  5. Application of high-resolution single-channel recording to functional studies of cystic fibrosis mutants.

    PubMed

    Cai, Zhiwei; Sohma, Yoshiro; Bompadre, Silvia G; Sheppard, David N; Hwang, Tzyh-Chang

    2011-01-01

    The patch-clamp technique is a powerful and versatile method to investigate the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel, its malfunction in disease and modulation by small molecules. Here, we discuss how the molecular behaviour of CFTR is investigated using high-resolution single-channel recording and kinetic analyses of channel gating. We review methods used to quantify how cystic fibrosis (CF) mutants perturb the biophysical properties and regulation of CFTR. By explaining the relationship between macroscopic and single-channel currents, we demonstrate how single-channel data provide molecular explanations for changes in CFTR-mediated transepithelial ion transport elicited by CF mutants. PMID:21594800

  6. Application of High-Resolution Single-Channel Recording to Functional Studies of Cystic Fibrosis Mutants

    PubMed Central

    Cai, Zhiwei; Sohma, Yoshiro; Bompadre, Silvia G.; Sheppard, David N.; Hwang, Tzyh-Chang

    2016-01-01

    The patch-clamp technique is a powerful and versatile method to investigate the cystic fibrosis transmem-brane conductance regulator (CFTR) Cl− channel, its malfunction in disease and modulation by small molecules. Here, we discuss how the molecular behaviour of CFTR is investigated using high-resolution single-channel recording and kinetic analyses of channel gating. We review methods used to quantify how cystic fibrosis (CF) mutants perturb the biophysical properties and regulation of CFTR. By explaining the relationship between macroscopic and single-channel currents, we demonstrate how single-channel data provide molecular explanations for changes in CFTR-mediated transepithelial ion transport elicited by CF mutants. PMID:21594800

  7. Characterising the Last Interglacial: High-resolution palaeoclimatic records from the Mediterranean

    NASA Astrophysics Data System (ADS)

    Milner, Alice; Martrat, Belen; Skinner, Luke; Tzedakis, Polychronis; Grimalt, Joan

    2013-04-01

    Evidence of abrupt climate variability during the Last Interglacial (approximately equivalent to MIS 5e) and glacial inception is detected in a variety of palaeoarchives but the nature of the variability is not yet satisfactorily resolved, nor are the causes fully understood. To improve our understanding of the climatic forcing and response to abrupt variability, detailed palaeoclimate data from a range of sites are required. The sensitivity of the Mediterranean region to global climate change makes it an ideal location from which to investigate variability during interglacials on decadal to millennial- timescales. We present a high-resolution palaeoclimatic record from the Central Mediterranean Sea with an aim of better understanding interglacial climate evolution and variability in the Mediterranean and its links to high- and low-latitude climate systems. High-resolution alkenone- and faunal-based sea surface temperature and pollen data are presented for the mid-latitude ODP Site 963 in the Strait of Sicily. This coupled terrestrial-marine record allows in situ assessment of the leads and lags of climate change in the terrestrial and marine environments. The new data provide evidence for intra-interglacial variability both on land and in the ocean, with a similar level of climate variability to that seen in the North Atlantic marine and Greenland ice core records. Our results suggests a close relation between the high northern latitudes and the Mediterranean during the Last Interglacial.

  8. Fiber optic cable-based high-resolution, long-distance VGA extenders

    NASA Astrophysics Data System (ADS)

    Rhee, Jin-Geun; Lee, Iksoo; Kim, Heejoon; Kim, Sungjoon; Koh, Yeon-Wan; Kim, Hoik; Lim, Jiseok; Kim, Chur; Kim, Jungwon

    2013-02-01

    Remote transfer of high-resolution video information finds more applications in detached display applications for large facilities such as theaters, sports complex, airports, and security facilities. Active optical cables (AOCs) provide a promising approach for enhancing both the transmittable resolution and distance that standard copper-based cables cannot reach. In addition to the standard digital formats such as HDMI, the high-resolution, long-distance transfer of VGA format signals is important for applications where high-resolution analog video ports should be also supported, such as military/defense applications and high-resolution video camera links. In this presentation we present the development of a compressionless, high-resolution (up to WUXGA, 1920x1200), long-distance (up to 2 km) VGA extenders based on serialized technique. We employed asynchronous serial transmission and clock regeneration techniques, which enables lower cost implementation of VGA extenders by removing the necessity for clock transmission and large memory at the receiver. Two 3.125-Gbps transceivers are used in parallel to meet the required maximum video data rate of 6.25 Gbps. As the data are transmitted asynchronously, 24-bit pixel clock time stamp is employed to regenerate video pixel clock accurately at the receiver side. In parallel to the video information, stereo audio and RS-232 control signals are transmitted as well.

  9. Optimal site selection for a high-resolution ice core record in East Antarctica

    NASA Astrophysics Data System (ADS)

    Vance, Tessa R.; Roberts, Jason L.; Moy, Andrew D.; Curran, Mark A. J.; Tozer, Carly R.; Gallant, Ailie J. E.; Abram, Nerilie J.; van Ommen, Tas D.; Young, Duncan A.; Grima, Cyril; Blankenship, Don D.; Siegert, Martin J.

    2016-03-01

    Ice cores provide some of the best-dated and most comprehensive proxy records, as they yield a vast and growing array of proxy indicators. Selecting a site for ice core drilling is nonetheless challenging, as the assessment of potential new sites needs to consider a variety of factors. Here, we demonstrate a systematic approach to site selection for a new East Antarctic high-resolution ice core record. Specifically, seven criteria are considered: (1) 2000-year-old ice at 300 m depth; (2) above 1000 m elevation; (3) a minimum accumulation rate of 250 mm years-1 IE (ice equivalent); (4) minimal surface reworking to preserve the deposited climate signal; (5) a site with minimal displacement or elevation change in ice at 300 m depth; (6) a strong teleconnection to midlatitude climate; and (7) an appropriately complementary relationship to the existing Law Dome record (a high-resolution record in East Antarctica). Once assessment of these physical characteristics identified promising regions, logistical considerations (for site access and ice core retrieval) were briefly considered. We use Antarctic surface mass balance syntheses, along with ground-truthing of satellite data by airborne radar surveys to produce all-of-Antarctica maps of surface roughness, age at specified depth, elevation and displacement change, and surface air temperature correlations to pinpoint promising locations. We also use the European Centre for Medium-Range Weather Forecast ERA 20th Century reanalysis (ERA-20C) to ensure that a site complementary to the Law Dome record is selected. We find three promising sites in the Indian Ocean sector of East Antarctica in the coastal zone from Enderby Land to the Ingrid Christensen Coast (50-100° E). Although we focus on East Antarctica for a new ice core site, the methodology is more generally applicable, and we include key parameters for all of Antarctica which may be useful for ice core site selection elsewhere and/or for other purposes.

  10. Optimal site selection for a high resolution ice core record in East Antarctica

    NASA Astrophysics Data System (ADS)

    Vance, T.; Roberts, J.; Moy, A.; Curran, M.; Tozer, C.; Gallant, A.; Abram, N.; van Ommen, T.; Young, D.; Grima, C.; Blankenship, D.; Siegert, M.

    2015-11-01

    Ice cores provide some of the best dated and most comprehensive proxy records, as they yield a vast and growing array of proxy indicators. Selecting a site for ice core drilling is nonetheless challenging, as the assessment of potential new sites needs to consider a variety of factors. Here, we demonstrate a systematic approach to site selection for a new East Antarctic high resolution ice core record. Specifically, seven criteria are considered: (1) 2000 year old ice at 300 m depth, (2) above 1000 m elevation, (3) a minimum accumulation rate of 250 mm yr-1 IE, (4) minimal surface re-working to preserve the deposited climate signal, (5) a site with minimal displacement or elevation change of ice at 300 m depth, (6) a strong teleconnection to mid-latitude climate and (7) an appropriately complementary relationship to the existing Law Dome record (a high resolution record in East Antarctica). Once assessment of these physical characteristics identified promising regions, logistical considerations (for site access and ice core retrieval) were briefly considered. We use Antarctic surface mass balance syntheses, along with ground-truthing of satellite data by airborne radar surveys to produce all-of-Antarctica maps of surface roughness, age at specified depth, elevation and displacement change and surface air temperature correlations to pinpoint promising locations. We also use the European Centre for Medium-Range Weather Forecast ERA 20th Century reanalysis (ERA-20C) to ensure a site complementary to the Law Dome record is selected. We find three promising sites in the Indian Ocean sector of East Antarctica in the coastal zone from Enderby Land to the Ingrid Christensen Coast (50-100° E). Although we focus on East Antarctica for a new ice core site, the methodology is more generally applicable and we include key parameters for all of Antarctica which may be useful for ice core site selection elsewhere and/or for other purposes.

  11. Quasi-static high-resolution magnetic-field detection based on dielectric optical resonators

    NASA Astrophysics Data System (ADS)

    Ioppolo, Tindaro; Rubino, Edoardo

    2013-06-01

    In this paper we present a high resolution magnetic field sensor that is based on the perturbation of the optical modes (whispering gallery mode, WGM) of a spherical dielectric resonator. The optical resonator is side coupled to a tapered single mode optical fiber. One side of the optical fiber is coupled to a distribute feedback diode laser, while the other end is connected to a photodiode. The optical modes of the dielectric cavity are perturbed using a metglas sheet that is in contact with the resonator. When the metglas sheet is exposed to an external magnetic field it elongates perturbing the optical modes of the dielectric cavity. This in turn leads to a shift in the optical resonances. By measuring the induced WGM shift the magnetic field can be measured. Preliminary results show sensor resolution of a few nanoteslas.

  12. Optical design of a versatile FIRST high-resolution near-IR spectrograph

    NASA Astrophysics Data System (ADS)

    Zhao, Bo; Ge, Jian

    2012-09-01

    We report the update optical design of a versatile FIRST high resolution near IR spectrograph, which is called Florida IR Silicon immersion grating spectromeTer (FIRST). This spectrograph uses cross-dispersed echelle design with white pupils and also takes advantage of the image slicing to increase the spectra resolution, while maintaining the instrument throughput. It is an extremely high dispersion R1.4 (blazed angle of 54.74°) silicon immersion grating with a 49 mm diameter pupil is used as the main disperser at 1.4μm -1.8μm to produce R=72,000 while an R4 echelle with the same pupil diameter produces R=60,000 at 0.8μm -1.35μm. Two cryogenic Volume Phase Holographic (VPH) gratings are used as cross-dispersers to allow simultaneous wavelength coverage of 0.8μm -1.8μm. The butterfly mirrors and dichroic beamsplitters make a compact folding system to record these two wavelength bands with a 2kx2k H2RG array in a single exposure. By inserting a mirror before the grating disperser (the SIG and the echelle), this spectrograph becomes a very efficient integral field 3-D imaging spectrograph with R=2,000-4,000 at 0.8μm-1.8μm by coupling a 10x10 telescope fiber bundle with the spectrograph. Details about the optical design and performance are reported.

  13. Optimizing planar lipid bilayer single-channel recordings for high resolution with rapid voltage steps.

    PubMed Central

    Wonderlin, W F; Finkel, A; French, R J

    1990-01-01

    We describe two enhancements of the planar bilayer recording method which enable low-noise recordings of single-channel currents activated by voltage steps in planar bilayers formed on apertures in partitions separating two open chambers. First, we have refined a simple and effective procedure for making small bilayer apertures (25-80 micrograms diam) in plastic cups. These apertures combine the favorable properties of very thin edges, good mechanical strength, and low stray capacitance. In addition to enabling formation of small, low-capacitance bilayers, this aperture design also minimizes the access resistance to the bilayer, thereby improving the low-noise performance. Second, we have used a patch-clamp headstage modified to provide logic-controlled switching between a high-gain (50 G omega) feedback resistor for high-resolution recording and a low-gain (50 M omega) feedback resistor for rapid charging of the bilayer capacitance. The gain is switched from high to low before a voltage step and then back to high gain 25 microseconds after the step. With digital subtraction of the residual currents produced by the gain switching and electrostrictive changes in bilayer capacitance, we can achieve a steady current baseline within 1 ms after the voltage step. These enhancements broaden the range of experimental applications for the planar bilayer method by combining the high resolution previously attained only with small bilayers formed on pipette tips with the flexibility of experimental design possible with planar bilayers in open chambers. We illustrate application of these methods with recordings of the voltage-step activation of a voltage-gated potassium channel. PMID:1698470

  14. Clinical in vivo two-photon microendoscopy for intradermal high-resolution imaging with GRIN optics

    NASA Astrophysics Data System (ADS)

    König, Karsten; Ehlers, Alexander; Riemann, Iris; Schenkl, Selma; Messerschmidt, Bernhard; Bückle, Rainer; Le Harzic, Ronan; Elsner, Peter; Kaatz, Martin

    2007-02-01

    Multiphoton tomography with the clinical femtosecond laser system DermaInspect has become an important non-invasive high resolution imaging tool for skin research, melanoma detection, and in situ drug monitoring of pharmaceutical and cosmetical components. The detection of endogenous fluorophores and SHG active biostructures such as mitochondrial NAD(P)H, melanin in melancytes and basal cells, as well as the extracellular matrix components elastin and collagen is achieved with submicron resolution when using high NA focusing optics. So far, the working distance was limited to 200 µm. In addition, the focusing optics was large in diameter (2-3 cm). Here we report for the first time on clinical deep-tissue high-resolution imaging with a novel high NA rigid GRIN microendoscope which extends the potential of clinical multiphoton tomography significantly. We performed the very first clinical in vivo measurements with two-photon endoscopes and studied wounds of patients with ulcus cruris.

  15. Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities

    PubMed Central

    Butterfield, Jacqualine; Hong, Weili; Mershon, Leslie; Vershinin, Michael

    2013-01-01

    High resolution microscope systems with optical traps allow for precise manipulation of various refractive objects, such as dielectric beads 1 or cellular organelles 2,3, as well as for high spatial and temporal resolution readout of their position relative to the center of the trap. The system described herein has one such "traditional" trap operating at 980 nm. It additionally provides a second optical trapping system that uses a commercially available holographic package to simultaneously create and manipulate complex trapping patterns in the field of view of the microscope 4,5 at a wavelength of 1,064 nm. The combination of the two systems allows for the manipulation of multiple refractive objects at the same time while simultaneously conducting high speed and high resolution measurements of motion and force production at nanometer and piconewton scale. PMID:23629580

  16. InSAR Forensics: Tracing InSAR Scatterers in High Resolution Optical Image

    NASA Astrophysics Data System (ADS)

    Wang, Yuanyuan; Zhu, XiaoXiang

    2015-05-01

    This paper presents a step towards a better interpretation of the scattering mechanism of different objects and their deformation histories in SAR interferometry (InSAR). The proposed technique traces individual SAR scatterer in high resolution optical images where their geometries, materials, and other properties can be better analyzed and classified. And hence scatterers of a same object can be analyzed in group, which brings us to a new level of InSAR deformation monitoring.

  17. A new high-resolution record of Holocene geomagnetic secular variation from New Zealand

    NASA Astrophysics Data System (ADS)

    Turner, G. M.; Howarth, J. D.; de Gelder, G. I. N. O.; Fitzsimons, S. J.

    2015-11-01

    We present the first full Holocene palaeomagnetic secular variation record from New Zealand. The 11 500 year-long record, from the sediments of Mavora Lakes, comprises composite declination, inclination and relative palaeointensity logs, compiled from two six-metre long cores and the uppermost 1.5 m of another. An age model has been developed from 28 AMS radiocarbon age determinations on fragments of southern beech (Lophozonia menziesii and Fuscospora cliffortioides) leaves. The excellent between-core correlation in all three components of the field results in a high-resolution palaeosecular variation record, with precise and accurate age control. The variations change in character from high amplitude in-phase declination and inclination swings in the earliest part of the record to low amplitude variations in the middle part and declination and inclination swings that are 90° out of phase, leading to broad looping of the vector in the upper part of the record, that is consistent with westward drifting sources in the outer core. The present-day field at the site (Dec = 24.2°E, Inc = - 70.7 °, F = 59 μT) represents a rare steep and easterly extreme direction, but close to average intensity. The palaeointensity is inferred to have varied between about 40 and 90 μT, with variations that, to some extent, mirror variations in the virtual axial geomagnetic dipole moment seen from global data, but also show some notable differences, particularly in the past few thousand years.

  18. The Potential for High-Resolution Palaeoclimate Records of the Pliocene from Speleothems

    NASA Astrophysics Data System (ADS)

    Drysdale, R.; Woodhead, J. D.; Hellstrom, J. C.

    2014-12-01

    The Pliocene is widely regarded as a useful analogue for future greenhouse warming and is thus an important interval for palaeoclimate study. Much of what we know about climate through the Pliocene comes from marine sediments. However, we know relatively little about interannual to interdecadal variability, yet reconstructing Pliocene climate at such resolution has the potential to yield important information for testing climate models. Recent advances in uranium-lead (U-Pb) dating have paved the way for investigating Pliocene (and older) high-resolution palaeoclimate records from speleothems. We present such an example from the Nullarbor Plain, Australia. The Nullabor is an emerged, karstified platform of Eocene to Miocene limestones situated on the arid southern fringe of the Australian continent. Caves developed in the karst preserve a large archive of ancient calcite speleothems, in stark contrast to the virtual lack of calcite speleothems today. U-Pb radiometric dating of these speleothems reveals that most the growth occurred during the Pliocene. The geochemistry of these speleothems suggests that the Nullarbor was a wetter and more-vegetated environment at the time. High-resolution geochemical analyses and fluorescence microscopy show that some of the speleothems are almost certainly annually laminated, and contain multi-proxy signatures similar to Holocene speleothems that are commonly associated with hydrological processes. We present a series of encouraging preliminary results from several specimens.

  19. High-resolution records of non-dipole variations derived from volcanic edifices

    NASA Astrophysics Data System (ADS)

    de Groot, L. V.; Biggin, A. J.; Dekkers, M. J.

    2013-12-01

    Our understanding of the short-term behavior of the Earth's magnetic field is currently mainly hampered by a lack of coeval high-resolution records of geomagnetic intensity variations that are well distributed over the globe. Lavas are the only recorder of short-term fluctuations of the geomagnetic field that are available for all parts of the world and on geological time scales. Therefore, many efforts have been made to improve the methodology to obtain reliable estimates of the paleointensity from igneous rocks over the past decades. It is well known that some paleointensity methods will work on certain lavas with specific thermomagnetic behavior, but fail for others. We therefore propose a ';multi-method paleointensity approach' that consists of Thellier-style, multispecimen-style, and calibrated pseudo-Thellier-style (AGU fall 2012 contribution GP43A-1122, submitted) experiments to construct high-resolution records of regional variations in the intensity of the Earth's magnetic field. With our new approach we obtain a reliable estimate of the paleointensity for 60-70 percent of all cooling units sampled. By applying our new approach to suites of lavas from Hawai'i (USA), and the Canary Islands (Spain) we obtain important constraints for short-term local geomagnetic intensity highs, and insight into their possible driving mechanisms. Our new record for Hawai'i indicates that, approximately 1000 years ago, the local field intensity increased on the order of 50% for 200 years - a qualitatively similar phenomenon observed 200 years earlier in western Europe (Gallet et al., 2005; Gómez-Paccard et al., 2012) and 500 years later in southwestern USA (Bowles et al., 2002). When these records are combined with a record for Japan (Yu, 2012), a coherent picture emerges that includes the dipole component decaying steadily since at least 1000 years ago. Superimposed onto this decay are strong but shorter-term intensity variations at a regional level whose asynchronicity

  20. High-resolution records of non-dipole variations derived from volcanic edifices

    NASA Astrophysics Data System (ADS)

    de Groot, Lennart; Biggin, Andrew; Dekkers, Mark

    2014-05-01

    Our understanding of the short-term behavior of the Earth's magnetic field is currently mainly hampered by a lack of coeval high-resolution records of geomagnetic intensity variations that are well distributed over the globe. Lavas are the only recorder of short-term fluctuations of the geomagnetic field that are available for all parts of the world and on geological time scales. Therefore, many efforts have been made to improve the methodology to obtain reliable estimates of the paleointensity from igneous rocks over the past decades. It is well known that some paleointensity methods will work on certain lavas with specific thermomagnetic behaviour, but fail for others. We therefore propose a 'multi-method paleointensity approach' that consists of Thellier-style, multispecimen-style, and calibrated pseudo-Thellier-style experiments to construct high-resolution records of regional variations in the intensity of the Earth's magnetic field. With our new approach we obtain a reliable estimate of the paleointensity for 60-70 percent of all cooling units sampled. By applying our new approach to suites of lavas from Hawaii (USA), and the Canary Islands (Spain) we obtain important constraints for short-term local geomagnetic intensity highs, and insight into their possible driving mechanisms. Our new record for Hawai'i indicates that, approximately 1000 years ago, the local field intensity increased on the order of 50% for 200 years - a qualitatively similar phenomenon observed 200 years earlier in western Europe (Gallet et al., 2005; Gomez-Paccard et al., 2012) and 500 years later in southwestern USA (Bowles et al., 2002). When these records are combined with a record for Japan (Yu, 2012), a coherent picture emerges that includes the dipole component decaying steadily since at least 1000 years ago. Superimposed onto this decay are strong but shorter-term intensity variations at a regional level whose asynchronicity necessitates a highly non-dipolar nature. Our

  1. High-resolution record of pyrogenic polycyclic aromatic hydrocarbon deposition during the 20th century.

    PubMed

    Lima, Ana Lúcia C; Eglinton, Timothy I; Reddy, Christopher M

    2003-01-01

    A high-resolution record of polycyclic aromatic hydrocarbon (PAH) deposition in Rhode Island over the past approximately 180 years was constructed using a sediment core from the anoxic Pettaquamscutt River basin. The record showed significantly more structure than has hitherto been reported and revealed four distinct maxima in PAH flux. The characteristic increase in PAH flux at the turn of the 20th century was captured in detail, leading to an initial maximum prior to the Great Depression. The overall peak in PAH flux in the 1950s was followed by a maximum that immediately preceded the 1973 Organization of Petroleum Exporting Countries (OPEC) oil embargo. During the most recent portion of the record, an abrupt increase in PAH flux between 1996 and 1999 has been found to follow a period of near constant fluxes. Because source-diagnostic ratios indicate that petrogenic inputs are minor throughout the record, these trends are interpreted in terms of past variations in the magnitude and type of combustion processes. For the most recent PAH maximum, energy consumption data suggest that diesel fuel combustion, and hence traffic of heavier vehicles, is the most probable cause for the increase in PAH flux. Systematic variations in the relative abundance of individual PAHs in conjunction with the above changes in flux are interpreted in relation to the evolution of combustion processes. Coronene, retene, and perylene are notable exceptions, exhibiting unique down-core profiles. PMID:12542290

  2. High-resolution palaeomagnetic records of the Laschamp geomagnetic excursion from ODP Sites 1061 and 1062

    NASA Astrophysics Data System (ADS)

    Bourne, M. D.; Henderson, G. M.; Thomas, A. L.; Mac Niocaill, C.

    2012-12-01

    The Laschamp geomagnetic excursion (~41 ka) was a brief global deviation in geomagnetic field behaviour from that expected during normal secular variation. Previously published records suggest rapid changes in field direction and a concurrent substantial decrease in field intensity. We present here high-resolution palaeomagnetic records of the Laschamp excursion obtained from two Ocean Drilling Program (ODP) Sites 1061 and 1062 on the Blake-Bahama Outer Ridge (ODP Leg 172) and compare this record with previously published records of the Blake and Iceland Basin Excursions. Relatively high sedimentation rates (>10 cm kyr-1) at these locations allow the determination of transitional field behaviour during the excursion. Rather than assuming a constant sedimentation rate between assigned age tie-points, we employ measurements of 230Thxs concentration in the sediment to assess variations in the sedimentation rates through the core sections of interest. This allows us to better determine the temporal behaviour of the Laschamp excursion with greater accuracy and known uncertainty. The Laschamp excursion at this location appears to be much shorter in duration than the Blake and Iceland Basin excursions. Palaeomagnetic measurements of discrete samples from four cores reveal a single excursional feature, across an interval of 30 cm, associated with a broader palaeointensity low. The excursion is characterised by rapid transitions (less than 500 years) between a stable normal polarity and a partially-reversed, polarity. Peaks in inclination either side of the directional excursion indicate periods of time when the local field is dominated by vertical flux patches. Similar behaviour has been observed in records of the Iceland Basin Excursion from the same region. The palaeointensity record is in good agreement between the two sites. The palaeointensity record shows two minima, where the second dip in intensity is associated with a more limited directional deviation. Similar

  3. Low-power high-resolution 32-channel neural recording system.

    PubMed

    Yun, Xiao; Kim, Donghwi; Stanaćević, Milutin; Mainen, Zachary

    2007-01-01

    A design of low-power 32-channel neural recording system with on-chip high-resolution A/D converters is presented. A neural front-end including low-noise fully differential pre-amplifier, gain stage, and buffer consumes only 56 mu W. Two 13-bits extended counting A/D converters running at 512KHz sampling rate are integrated with 32 neural front-ends on a chip. The experimental prototype was designed in 0.6 microm CMOS process. With a 3.3V power supply, total power consumption of a chip is 22mW and the whole system occupies an area of 3mm x 3mm. PMID:18002470

  4. In Situ Visualization of Tears on Contact Lens Using Ultra High Resolution Optical Coherence Tomography

    PubMed Central

    Wang, Jianhua; Jiao, Shuliang; Ruggeri, Marco; Shousha, Mohammed Abou; Chen, Qi

    2012-01-01

    Objective To demonstrate the capability of directly visualizing the tear film on contact lenses using optical coherence tomography (OCT). Methods Six eyes of three healthy subjects wearing PureVision and ACUVUE Advance soft and Boston RGP hard contact lenses were imaged with a custom built, high speed, ultra-high resolution spectral domain optical coherence tomograph. Refresh Liquigel was used to demonstrate the effect of artificial tears on the tear film. Results Ultra high resolution images of the pre- and post-lens films were directly visualized when each lens was inserted onto the eye. After the instillation of artificial tears during lens wear, the tear film was thicker. The post-lens tear film underneath the lens edge was clearly shown. Interactions between the lens edges and the ocular surface were obtained for each of the lens types and base curves. With a contrast enhancement agent, tear menisci on the contact lenses around the upper and lower eyelids were highlighted. With hard contact lenses, the tear film was visualized clearly and changed after a blink when the lens was pulled up by the lid. Conclusions Ultra-high resolution OCT is a potentially promising technique for imaging tears around contact lenses. This successful demonstration of in situ post-lens tear film imaging suggests that OCT could open a new era in studying tear dynamics during contact lens wear. The novel method may lead to new ways of evaluating contact lens fitting. PMID:19265323

  5. Far-field high resolution effects and manipulating of electromagnetic waves based on transformation optics

    NASA Astrophysics Data System (ADS)

    Ji, XueBin; Zang, XiaoFei; Li, Zhou; Shi, Cheng; Chen, Lin; Cai, Bin; Zhu, YiMing

    2015-05-01

    Based on the transformation optics (TO) and the effective medium theory (EMT), a new illusion media with homogeneous and isotropic materials is proposed to realize the far-field high resolution effects. When two point sources with the separation distance of λ0 / 4 are covered with the illusion media (λ0 is the free-space wavelength), the corresponding far-field pattern is equivalent to the case of two point sources with the separation distance larger than λ0 / 2 in free space, leading to the far-field high resolution effects (in free space, the separation distance of λ0 / 4 is less than half-wavelength, and thus the two point sources cannot be distinguished from each other). Furthermore, such illusion media can be applied to design tunable high-directivity antenna and an angle-dependent floating carpet cloak. Full wave simulations are carried out to verify the performance of our device.

  6. High-resolution and wide-bandwidth light intensity fiber optic displacement sensor for MEMS metrology.

    PubMed

    Orłowska, Karolina; Świątkowski, Michał; Kunicki, Piotr; Kopiec, Daniel; Gotszalk, Teodor

    2016-08-01

    We report on the design, properties, and applications of a high-resolution and wide-bandwidth light intensity fiber optic displacement sensor for microelectromechanical system (MEMS) metrology. There are two types of structures that the system is dedicated to: vibrating with both high and low frequencies. In order to ensure high-frequency and high-resolution measurements, frequency down mixing and selective signal processing were applied. The obtained effective measuring bandwidth ranges from single hertz to 1 megahertz. The achieved resolution presented here is 116  pm/Hz1/2 and 138  pm/Hz1/2 for low-frequency and high-frequency operation modes, respectively, whereas the measurement of static displacement is 100 μm. PMID:27505377

  7. High-resolution full-field optical coherence microscopy using a broadband light-emitting diode.

    PubMed

    Ogien, Jonas; Dubois, Arnaud

    2016-05-01

    High-resolution full-field optical coherence microscopy (FF-OCM) is demonstrated using a single broadband light-emitting diode (LED). The characteristics of the LED-illumination FF-OCM system are measured and compared to those obtained using a halogen lamp, the light source of reference in FF-OCM. Both light sources yield identical performance in terms of spatial resolution and detection sensitivity, using the same setup and camera. In particular, an axial resolution of 0.7 μm (in water) is reached. A Xenopus laevis tadpole and ex-vivo human skin have been imaged using both sources, resulting in similar images, showing for the first time that LEDs could favorably replace halogen lamps in high-resolution FF-OCM for biomedical imaging. PMID:27137603

  8. A high-resolution δ18O record and Mediterranean climate variability

    NASA Astrophysics Data System (ADS)

    Taricco, C.; Vivaldo, G.; Alessio, S.; Rubinetti, S.; Mancuso, S.

    2015-03-01

    A high-resolution, well-dated foraminiferal δ18O record from a shallow-water core drilled from the Gallipoli Terrace in the Gulf of Taranto (Ionian Sea), previously measured over the last two millennia, has been extended to cover 707 BC-AD 1979. Spectral analysis of this series, performed using singular-spectrum analysis (SSA) and other classical and advanced methods, strengthens the results obtained analysing the shorter δ18O profile, detecting the same highly significant oscillations of about 600, 380, 170, 130 and 11 years, respectively explaining about 12, 7, 5, 2 and 2% of the time series total variance, plus a millennial trend (18% of the variance). The comparison with the results of multi-channel singular-spectrum analysis (MSSA) applied to a data set of 26 Northern Hemisphere (NH) temperature-proxy records shows that NH temperature anomalies share with our local record a~long-term trend and a bicentennial (170-year period) cycle. These two variability modes, previously identified as temperature-driven, are the most powerful modes in the NH temperature data set. Both the long-term trends and the bicentennial oscillations, when reconstructed locally and hemispherically, show coherent phases. Furthermore, the corresponding local and hemispheric amplitudes are comparable if changes in the precipitation-evaporation balance of the Ionian sea, presumably associated with temperature changes, are taken into account.

  9. A high-resolution δ18O record and Mediterranean climate variability

    NASA Astrophysics Data System (ADS)

    Taricco, C.; Vivaldo, G.; Alessio, S.; Rubinetti, S.; Mancuso, S.

    2014-10-01

    A~high-resolution, well-dated foraminiferal δ18O record from a shallow-water core drilled from the Gallipoli Terrace in the Gulf of Taranto (Ionian Sea), previously measured over the last two millennia, has been extended to cover 707 BC-1979 AD. Spectral analysis of this series, performed by Singular Spectrum Analysis (SSA) and other classical and advanced methods, strengthens the results obtained analysing the shorter δ18O profile, detecting the same highly significant oscillations of about 600 yr, 380 yr, 170 yr, 130 yr, and 11 yr, respectively explaining about 12%, 7%, 5%, 2% and 2% of the time series total variance, plus a millennial trend (18% of the variance). The comparison with the results of Multi-channel Singular Spectrum Analysis (MSSA) applied to a data set of 26 Northern Hemisphere (NH) temperature-proxy records shows that NH temperature anomalies share with our local record a long-term trend and a bicentennial cycle. These two variability modes, previously identified as temperature-driven, are the most powerful modes in the NH temperature data set. Both the long-term trends and the bicentennial oscillations, when reconstructed locally and hemispherically, show coherent phases. Also the corresponding local and hemispheric amplitudes are comparable, if changes in the precipitation-evaporation balance of the Ionian sea, presumably associated with temperature changes, are taken into account.

  10. Low Frequency Error Analysis and Calibration for High-Resolution Optical Satellite's Uncontrolled Geometric Positioning

    NASA Astrophysics Data System (ADS)

    Wang, Mi; Fang, Chengcheng; Yang, Bo; Cheng, Yufeng

    2016-06-01

    The low frequency error is a key factor which has affected uncontrolled geometry processing accuracy of the high-resolution optical image. To guarantee the geometric quality of imagery, this paper presents an on-orbit calibration method for the low frequency error based on geometric calibration field. Firstly, we introduce the overall flow of low frequency error on-orbit analysis and calibration, which includes optical axis angle variation detection of star sensor, relative calibration among star sensors, multi-star sensor information fusion, low frequency error model construction and verification. Secondly, we use optical axis angle change detection method to analyze the law of low frequency error variation. Thirdly, we respectively use the method of relative calibration and information fusion among star sensors to realize the datum unity and high precision attitude output. Finally, we realize the low frequency error model construction and optimal estimation of model parameters based on DEM/DOM of geometric calibration field. To evaluate the performance of the proposed calibration method, a certain type satellite's real data is used. Test results demonstrate that the calibration model in this paper can well describe the law of the low frequency error variation. The uncontrolled geometric positioning accuracy of the high-resolution optical image in the WGS-84 Coordinate Systems is obviously improved after the step-wise calibration.

  11. Ultra-high resolution spectral domain optical coherence tomography using supercontinuum light source

    NASA Astrophysics Data System (ADS)

    Lim, Yiheng; Yatagai, Toyohiko; Otani, Yukitoshi

    2016-04-01

    An ultra-high resolution spectral domain optical coherence tomography (SD-OCT) was developed using a cost-effective supercontinuum laser. A spectral filter consists of a dispersive prism, a cylindrical lens and a right-angle prism was built to transmit the wavelengths in range 680-940 nm to the OCT system. The SD-OCT has achieved 1.9 μm axial resolution and the sensitivity was estimated to be 91.5 dB. A zero-crossing fringes matching method which maps the wavelengths to the pixel indices of the spectrometer was proposed for the OCT spectral calibration. A double sided foam tape as a static sample and the tip of a middle finger as a biological sample were measured by the OCT. The adhesive and the internal structure of the foam of the tape were successfully visualized in three dimensions. Sweat ducts was clearly observed in the OCT images at very high resolution. To the best of our knowledge, this is the first demonstration of ultra-high resolution visualization of sweat duct by OCT.

  12. North Atlantic Holocene climate evolution recorded by high-resolution terrestrial and marine biomarker records

    NASA Astrophysics Data System (ADS)

    Moossen, Heiko; Bendle, James; Seki, Osamu; Quillmann, Ursula; Kawamura, Kimitaka

    2015-12-01

    Holocene climatic change is driven by a plethora of forcing mechanisms acting on different time scales, including: insolation, internal ocean (e.g. Atlantic Meridional Overturning Circulation; AMOC) and atmospheric (e.g. North Atlantic Oscillation; NAO) variability. However, it is unclear how these driving mechanisms interact with each other. Here we present five, biomarker based, paleoclimate records (air-, sea surface temperature and precipitation), from a fjordic sediment core, revealing North Atlantic terrestrial and marine climate in unprecedented detail. The Early Holocene (10.7-7.8 kyrs BP) is characterised by relatively high air temperatures while SSTs are dampened by melt water events, and relatively low precipitation. The Middle Holocene (7.8-3.2 kyrs BP) is characterised by peak SSTs, declining air temperatures and high precipitation. A pronounced marine thermal maximum occurs between ∼7-5.5 kyrs BP, 3000 years after the terrestrial thermal maximum, driven by melt water cessation and an accelerating AMOC. The neoglacial cooling, between 5.8 and 3.2 kyrs BP leads into the late Holocene. We demonstrate that an observed modern link between Icelandic precipitation variability during different NAO phases, may have existed from ∼7.5 kyrs BP. A simultaneous decoupling of both air, and sea surface temperature records from declining insolation at ∼3.2 kyrs BP may indicate a threshold, after which internal feedback mechanisms, namely the NAO evolved to be the primary drivers of Icelandic climate on centennial time-scales.

  13. High-resolution record of the Laschamp geomagnetic excursion at the Blake-Bahama Outer Ridge

    NASA Astrophysics Data System (ADS)

    Bourne, Mark D.; Mac Niocaill, Conall; Thomas, Alex L.; Henderson, Gideon M.

    2013-12-01

    Geomagnetic excursions are brief deviations of the geomagnetic field from behaviour expected during `normal secular' variation. The Laschamp excursion at ˜41 ka was one such deviation. Previously published records suggest rapid changes in field direction and a concurrent substantial decrease in field intensity associated with this excursion. Accurate dating of excursions, and determination of their durations from multiple locations, is vital to our understanding of global field behaviour during these deviations. We present here high-resolution palaeomagnetic records of the Laschamp excursion obtained from two Ocean Drilling Program (ODP) Sites, 1061 and 1062 on the Blake-Bahama Outer Ridge (ODP Leg 172). High sedimentation rates (˜30-40 cm kyr-1) at these locations allow determination of transitional field behaviour during the excursion. Palaeomagnetic measurements of discrete samples from four cores reveal a single excursional feature, across an interval of 30 cm, associated with a broader palaeointensity low. We determine the age and duration of the Laschamp excursion using a stratigraphy linked to the δ18O record from the Greenland ice cores. This chronology dates the Laschamp excursion at the Blake Ridge to 41.3 ka. The excursion is characterized by rapid transitions (less than 200 yr) between stable normal polarity and a partially reversed polarity state. The palaeointensity record is in good agreement between the two sites, revealing two prominent minima. The first minimum is associated with the Laschamp excursion at 41 ka and the second corresponds to the Mono Lake excursion at ˜35.5 ka. We determine that the directional excursion during the Laschamp at this location was no longer than ˜400 yr, occurring within a palaeointensity minimum that lasted 2000 yr. The Laschamp excursion at this location is much shorter in duration than the Blake and Iceland Basin excursions.

  14. High-resolution isotopic records from corals using ocean temperature and mass-spawning chronometers

    NASA Astrophysics Data System (ADS)

    Gagan, Michael K.; Chivas, Allan R.; Isdale, Peter J.

    1994-02-01

    We present a 6-year-long (1978-1984) delta O-18 and delta C-13 record from a Great Barrier Reef (Pandora Reef) Porites lutea coral based on near-weekly sample intervals. A sampling technique was designed to minimise any smoothing or distortion of the isotopic record due to complex coral growth, calyx architecture, and calcification at depth within the tissue layer. The arrival-time of the mid-winter minimum sea-surface temperature is very consistent (+/- 2 weeks) near Pandora Reef and provides an annual time-marker offering more precision than the traditional density band chronometer. The improved chronology and high-resolution record demonstrate that signal distortion in Porites, due to calcification within the tissue layer and variable intra-annual coral extension, is generally negligible. Also confirmed is that the arrival-time of monsoonal floods is precisely preserved (+/- 1 week) within the skeleton of Porites. The sensitivity of weekly sampling allows the detection of a subtle warm-to-cool sea-surface temperature anomaly which preceded, by more than one year, a similar temperature anomaly associated with the 1982-83 El Nino in the east Pacific. Sharply higher delta C-13 values coincide with the time of the annual coral mass-spawning event in the Great Barrier Reef. Recognition of this mass-spawning signal should simplify the interpretation of coral delta C-13 records and provides an additional, precise time-marker with which to adjust chronologies when intra-annual coral extension is not constant.

  15. Ultra-high resolution filter and optical field modulator based on a surface plasmon polariton.

    PubMed

    Wu, Wenjun; Yang, Junbo; Zhang, Jingjing; Huang, Jie; Chen, Dingbo; Wang, Hongqing

    2016-05-15

    A new filter structure and optical field modulator with ultra-high resolution based on plasmonic nano-cavity resonators is proposed and numerically investigated. The structure consists of a square nano-cavity resonator connected with several waveguides. All waveguides and cavity are etched on a silver film whose size is 1.1×0.75  μm. Compared with traditional filters, the FWHM (full width at half-maximum) of this structure's spectrum curve can be less than 7 nm; namely, the resolution has been greatly improved. The structure also presents the feature of an optical field modulator when both inputs are working simultaneously, and it provides a promising way to design and manufacture future optical logical device. PMID:27176990

  16. High-resolution X-ray imaging by polycapillary optics and lithium fluoride detectors combination

    NASA Astrophysics Data System (ADS)

    Hampai, D.; Dabagov, S. B.; Della Ventura, G.; Bellatreccia, F.; Magi, M.; Bonfigli, F.; Montereali, R. M.

    2011-12-01

    Novel results on high-resolution X-ray imaging by a table-top laboratory system based on lithium fluoride (LiF) imaging radiation detectors and a X-ray tube combined with polycapillary optics are reported for the first time. In this paper, imaging experiments of reference objects, as well as thick geological samples, show some of the potentialities of this approach for the development of a compact laboratory X-ray microscopy apparatus. The high spatial resolution and dynamic range of versatile LiF imaging detectors, based on optical reading of photoluminescence from X-ray-induced color centers in LiF crystals and films, allow us to use very simple contact imaging techniques. Promising applications can be foreseen in the fields of bio-medical imaging diagnostics, characterization of X-ray sources and optical elements, material science and photonics.

  17. Fusing electro-optic and infrared signals for high resolution night images

    NASA Astrophysics Data System (ADS)

    Huang, Xiaopeng; Netravali, Ravi; Man, Hong; Lawrence, Victor

    2012-03-01

    Electro-optic (EO) images exhibit the properties of high resolution and low noise level, while it is a challenge to distinguish objects at night through infrared (IR) images, especially for objects with a similar temperature. Therefore, we will propose a novel framework of IR image enhancement based on the information (e.g., edge) from EO images, which will result in high resolution IR images and help us distinguish objects at night. Superimposing the detected edge of the EO image onto the corresponding transformed IR image is our principal idea for the proposed framework. In this framework, we will adopt the theoretical point spread function (PSF) proposed by Russell C. Hardie et al. for our IR image system, which is contributed by the modulation transfer function (MTF) of a uniform detector array and the incoherent optical transfer function (OTF) of diffraction-limited optics. In addition, we will design an inverse filter in terms of the proposed PSF to conduct the IR image transformation. The framework requires four main steps, which are inverse filter-based IR image transformation, EO image edge detection, registration and superimposing of the obtained image pair. Simulation results will show the superimposed IR images.

  18. Optical design for a breadboard high-resolution spectrometer for SIRTF/IRS

    NASA Astrophysics Data System (ADS)

    Brown, Robert J.; Houck, James R.; van Cleve, Jeffrey E.

    1996-11-01

    The optical design of a breadboard high resolution infrared spectrometer for the IRS instrument on the SIRTF mission is discussed. The spectrometer uses a crossed echelle grating configuration to cover the spectral region from 10 to 20 micrometer with a resolving power of approximately equals 600. The all reflective spectrometer forms a nearly diffraction limited image of the two dimensional spectrum on a 128 multiplied by 128 arsenic doped silicon area array with 75 micrometer pixels. The design aspects discussed include, grating numerology, image quality, packaging and alignment philosophy.

  19. Progress on the Gemini High-Resolution Optical SpecTrograph (GHOST) design

    NASA Astrophysics Data System (ADS)

    Ireland, Michael; Anthony, Andre; Burley, Greg; Chisholm, Eric; Churilov, Vladimir; Dunn, Jennifer; Frost, Gabriella; Lawrence, Jon; Loop, David; McGregor, Peter; Martell, Sarah; McConnachie, Alan; McDermid, Richard M.; Pazder, John; Reshetov, Vlad; Robertson, J. G.; Sheinis, Andrew; Tims, Julia; Young, Peter; Zhelem, Ross

    2014-07-01

    The Gemini High-Resolution Optical SpecTrograph (GHOST) is the newest instrument being developed for the Gemini telescopes, in a collaboration between the Australian Astronomical Observatory (AAO), the NRC - Herzberg in Canada and the Australian National University (ANU). We describe the process of design optimisation that utilizes the unique strengths of the new partner, NRC - Herzberg, the design and need for the slit viewing camera system, and we describe a simplification for the lenslet-based slit reformatting. Finally, we out- line the updated project plan, and describe the unique scientific role this instrument will have in an international context, from exoplanets through to the distant Universe.

  20. High-resolution deep-tissue optical imaging using anti-Stokes phosphors

    NASA Astrophysics Data System (ADS)

    Popov, A. P.; Karmenyan, A. V.; Bykov, A. V.; Khaydukov, E. V.; Nechaev, A. V.; Bibikova, O. A.; Panchenko, V. Y.; Semchishen, V. A.; Seminogov, V. N.; Akhmanov, A. S.; Sokolov, V. I.; Kinnunen, M. T.; Tuchin, V. V.; Zvyagin, A. V.

    2013-06-01

    We report on the high-resolution deep-tissue imaging using novel water-dispersible upconversion nanoparticles (UCNPs) β-NaYF4:Yb3+:Tm3+. Luminescence from the UCNP embedded into tissue-mimicking phantoms at the depth of 4 mm epi-illuminated with 975-nm laser radiation was detected. Fiber-optic detection shows 2-times better resolution compared with that obtained using CCD-based imaging modality. The conversion efficiency of upconversion particles and their cytotoxicity to HeLa cells were also investigated and reported.

  1. Developmental and morphological studies in Japanese medaka with ultra-high resolution optical coherence tomography

    PubMed Central

    Gladys, Fanny Moses; Matsuda, Masaru; Lim, Yiheng; Jackin, Boaz Jessie; Imai, Takuto; Otani, Yukitoshi; Yatagai, Toyohiko; Cense, Barry

    2015-01-01

    We propose ultra-high resolution optical coherence tomography to study the morphological development of internal organs in medaka fish in the post-embryonic stages at micrometer resolution. Different stages of Japanese medaka were imaged after hatching in vivo with an axial resolution of 2.8 µm in tissue. Various morphological structures and organs identified in the OCT images were then compared with the histology. Due to the medaka’s close resemblance to vertebrates, including humans, these morphological features play an important role in morphogenesis and can be used to study diseases that also occur in humans. PMID:25780725

  2. High Resolution Spectroscopy of Naphthalene Calibrated by AN Optical Frequency Comb

    NASA Astrophysics Data System (ADS)

    Nishiyama, Akiko; Nakashima, Kazuki; Matsuba, Ayumi; Misono, Masatoshi

    2015-06-01

    In high-resolution molecular spectroscopy, the precise measure of the optical frequency is crucial to evaluate minute shifts and splittings of the energy levels. On the other hand, in such spectroscopy, thousands of spectral lines distributed over several wavenumbers have to be measured by a continuously scanning cw laser. Therefore, the continuously changing optical frequency of the scanning laser has to be determined with enough precision. To satisfy these contradictory requirements, we have been developed two types of high-resolution spectroscopic systems employing an optical frequency comb. One of the systems employs RF band-pass filters to generate equally spaced frequency markers for optical frequency calibration, and is appropriate for wide wavelength-range measurement with relatively high scanning rate.^a In the other system, the beat frequency between the optical frequency comb and the scanning laser is controlled by an acousto-optic frequency shifter. This system is suitable for more precise measurement, and enables detailed analyses of frequency characteristics of scanning laser.^b In the present study, we observe Doppler-free two-photon absorption spectra of A^1B1u (v_4 = 1) ← X^1A_g (v = 0) transition of naphthalene around 298 nm. The spectral lines are rotationally resolved and the resolution is about 100 kHz. For ^qQ transition, the rotational lines are assigned, and molecular constants in the excited state are determined. In addition, we analyze the origin of the measured linewidth and Coriolis interactions between energy levels. To determine molecular constants more precisely, we proceed to measure and analyze spectra of other transitions, such as ^sS transitions. ^a A. Nishiyama, D. Ishikawa, and M. Misono, J. Opt. Soc. Am. B 30, 2107 (2013). ^b A. Nishiyama, A. Matsuba, and M. Misono, Opt. Lett. 39, 4923 (2014).

  3. High-Resolution Glacial Discharge Records From Deep-Water Tidal Rhythmites in an Alaskan Fjord

    NASA Astrophysics Data System (ADS)

    Jackolski, C. L.; Cowan, E. A.; Jaeger, J. M.; Powell, R. D.

    2006-12-01

    In this study we have compiled and analyzed two high-resolution records of deep-water tidal rhythmites derived from glacial discharges. The rhythmites contain an average of 1 cm of sediment thickness per week during the melt-season. Two sediment cores over 17-m-long were collected from Muir Inlet, Glacier Bay National Park, Southeast Alaska, aboard the R/V Maurice Ewing in 2004 (EW0408). One core (core 60JC) was collected just north of the mouth of Wachusett Inlet. The other (core 62JC) was collected 3.8 km due north of 60JC in a separate basin more proximal to Muir and McBride Glaciers. In Glacier Bay, glacial retreat since the Little Ice Age Maximum is well constrained by historical mapping of glacial temini, and local climate records exist as well. The next step after establishing glacial discharge records is to test hypotheses relating glacial discharge to temperature and rainfall. The cores were subsampled with ODP-style u-channels and scanned for magnetic susceptibility and bulk density. X-radiographs of u-channels were collected to observe small-scale (42 microns/pixel) density differences between silt and mud laminae. Spring-neap tidal packages, representing 2-week periods during summer, are visible because of closely-spaced bounding silt laminae. Seasonality is marked by winter gravelly mud (diamicton) beds and/or spring plankton blooms. Spring-neap packages and seasonal markers were considered jointly in order to construct two mostly continuous records of spring-neap packages and melt- season deposits in each core. Secondarily, melt-season duration, which we define as the number of spring- neap packages occurring in a melt-season, was determined. We found that core 60JC extends back 83 years. 75 of those years were identified as having complete, contiguous melt-season deposits that are, on average, 17 cm thick and contain 8.3 spring-neap packages. Core 62JC extends back 62 years. 57 of those years were identified as having complete, contiguous melt

  4. A High Resolution Stable Isotope Record of the lower Danian from the Caravaca Section (SE Spain)

    NASA Astrophysics Data System (ADS)

    Moretti, M.; Galeotti, S.; Lanci, L.; Pagani, M.

    2014-12-01

    Early Eocene hyperthermals represent large perturbations of the global carbon cycle characterized by significant global temperature increases. The best known and largest episode is represented by the Paleocene Eocene Thermal Maximum (PETM) also known as the Eocene Thermal Maximum 1 (ETM1), which occurs at ~55.7 Ma. Two other major episodes include the ETM2 (~55.7 Ma) and the ETM3 (~54.3 Ma). Available geochemical and sedimentary records indicates that other hyperthermal events might have occurred during the Paleocene, including the DanC2 event at ~ 65.4 Ma (Quillévéré et al., 2008), Top C27N event at ~61.6 Ma (Westerhold et al., 2008) and the Lower C29N event (Coccioni et al., 2012). A key feature of hyperthermals is the abrupt negative shift in δ13C values, which is interpreted to reflect the release of a large mass of 13C-depleted carbon in the form of CH4 and/or CO2 into the atmosphere-ocean system, causing dramatic warming and ocean acidification (Zachos et al., 2005). Here we present a high resolution biostratigraphic, magnetic susceptibility, and geochemical record of the lower Danian from the Caravaca section (SE Spain). By combining inorganic and organic geochimistry proxies we were able to identify the Dan-C2 event in our section and the Lower C29N event. A sampling spacing of 5 cm corresponding to ~3 kyr in the time domain based on the average sedimentation rate of 1.5 cm/kyr (Smit, 2004) provides a well resolved record of the two events. This resolution can be used to detect short-term periodicity and, therefore, accurately determine the timing of the two events and their duration.

  5. Automatic Near-Real-Time Image Processing Chain for Very High Resolution Optical Satellite Data

    NASA Astrophysics Data System (ADS)

    Ostir, K.; Cotar, K.; Marsetic, A.; Pehani, P.; Perse, M.; Zaksek, K.; Zaletelj, J.; Rodic, T.

    2015-04-01

    In response to the increasing need for automatic and fast satellite image processing SPACE-SI has developed and implemented a fully automatic image processing chain STORM that performs all processing steps from sensor-corrected optical images (level 1) to web-delivered map-ready images and products without operator's intervention. Initial development was tailored to high resolution RapidEye images, and all crucial and most challenging parts of the planned full processing chain were developed: module for automatic image orthorectification based on a physical sensor model and supported by the algorithm for automatic detection of ground control points (GCPs); atmospheric correction module, topographic corrections module that combines physical approach with Minnaert method and utilizing anisotropic illumination model; and modules for high level products generation. Various parts of the chain were implemented also for WorldView-2, THEOS, Pleiades, SPOT 6, Landsat 5-8, and PROBA-V. Support of full-frame sensor currently in development by SPACE-SI is in plan. The proposed paper focuses on the adaptation of the STORM processing chain to very high resolution multispectral images. The development concentrated on the sub-module for automatic detection of GCPs. The initially implemented two-step algorithm that worked only with rasterized vector roads and delivered GCPs with sub-pixel accuracy for the RapidEye images, was improved with the introduction of a third step: super-fine positioning of each GCP based on a reference raster chip. The added step exploits the high spatial resolution of the reference raster to improve the final matching results and to achieve pixel accuracy also on very high resolution optical satellite data.

  6. High-resolution temperature sensing in the Dead Sea using fiber optics

    NASA Astrophysics Data System (ADS)

    Arnon, A.; Lensky, N. G.; Selker, J. S.

    2014-02-01

    The thermal stratification of the Dead Sea was observed in high spatial and temporal resolution by means of fiber-optics temperature sensing. The aim of the research was to employ the novel high-resolution profiler in studying the dynamics of the thermal structure of the Dead Sea and the related processes including the investigation of the metalimnion fluctuations. The 18 cm resolution profiling system was placed vertically through the water column supported by a buoy 450 m from shore, from 2 m above to 53 m below the water surface (just above the local seafloor), covering the entire seasonal upper layer (the metalimnion had an average depth of ˜20 m). Temperature profiles were recorded every 5 min. The May to July 2012 data set allowed quantitative investigation of the thermal morphology dynamics, including objective definitions of key locations within the metalimnion based on the temperature depth profile and its first and second depth derivatives. Analysis of the fluctuation of the defined metalimnion locations showed strong anticorrelation to measured sea level fluctuations. The slope of the sea level versus metalimnion depth was found to be related to the density ratio of the upper layer and the underlying main water body, according to the prediction of a two-layer model. The heat content of the entire water column was calculated by integrating the temperature profiles. The vertically integrated apparent heat content was seen to vary by 50% in a few hours. These fluctuations were not correlated to the atmospheric heat fluxes, nor to the momentum transfer, but were highly correlated to the metalimnion and the sea level fluctuations (r = 0.84). The instantaneous apparent heat flux was 3 orders of magnitude larger than that delivered by radiation, with no direct correlation to the frequency of radiation and wind in the lake. This suggests that the source of the momentary heat flux is lateral advection due to internal waves (with no direct relation to the diurnal

  7. Experimental validation of a high-resolution diffuse optical imaging modality: photomagnetic imaging

    NASA Astrophysics Data System (ADS)

    Nouizi, Farouk; Luk, Alex; Thayer, Dave; Lin, Yuting; Ha, Seunghoon; Gulsen, Gultekin

    2016-01-01

    We present experimental results that validate our imaging technique termed photomagnetic imaging (PMI). PMI illuminates the medium under investigation with a near-infrared light and measures the induced temperature increase using magnetic resonance imaging. A multiphysics solver combining light and heat propagation is used to model spatiotemporal distribution of temperature increase. Furthermore, a dedicated PMI reconstruction algorithm has been developed to reveal high-resolution optical absorption maps from temperature measurements. Being able to perform measurements at any point within the medium, PMI overcomes the limitations of conventional diffuse optical imaging. We present experimental results obtained on agarose phantoms mimicking biological tissue with inclusions having either different sizes or absorption contrasts, located at various depths. The reconstructed images show that PMI can successfully resolve these inclusions with high resolution and recover their absorption coefficient with high-quantitative accuracy. Even a 1-mm inclusion located 6-mm deep is recovered successfully and its absorption coefficient is underestimated by only 32%. The improved PMI system presented here successfully operates under the maximum skin exposure limits defined by the American National Standards Institute, which opens up the exciting possibility of its future clinical use for diagnostic purposes.

  8. New optical sensor systems for high-resolution satellite, airborne and terrestrial imaging systems

    NASA Astrophysics Data System (ADS)

    Eckardt, Andreas; Börner, Anko; Lehmann, Frank

    2007-10-01

    The department of Optical Information Systems (OS) at the Institute of Robotics and Mechatronics of the German Aerospace Center (DLR) has more than 25 years experience with high-resolution imaging technology. The technology changes in the development of detectors, as well as the significant change of the manufacturing accuracy in combination with the engineering research define the next generation of spaceborne sensor systems focusing on Earth observation and remote sensing. The combination of large TDI lines, intelligent synchronization control, fast-readable sensors and new focal-plane concepts open the door to new remote-sensing instruments. This class of instruments is feasible for high-resolution sensor systems regarding geometry and radiometry and their data products like 3D virtual reality. Systemic approaches are essential for such designs of complex sensor systems for dedicated tasks. The system theory of the instrument inside a simulated environment is the beginning of the optimization process for the optical, mechanical and electrical designs. Single modules and the entire system have to be calibrated and verified. Suitable procedures must be defined on component, module and system level for the assembly test and verification process. This kind of development strategy allows the hardware-in-the-loop design. The paper gives an overview about the current activities at DLR in the field of innovative sensor systems for photogrammetric and remote sensing purposes.

  9. Ship detection for high resolution optical imagery with adaptive target filter

    NASA Astrophysics Data System (ADS)

    Ju, Hongbin

    2015-10-01

    Ship detection is important due to both its civil and military use. In this paper, we propose a novel ship detection method, Adaptive Target Filter (ATF), for high resolution optical imagery. The proposed framework can be grouped into two stages, where in the first stage, a test image is densely divided into different detection windows and each window is transformed to a feature vector in its feature space. The Histograms of Oriented Gradients (HOG) is accumulated as a basic feature descriptor. In the second stage, the proposed ATF highlights all the ship regions and suppresses the undesired backgrounds adaptively. Each detection window is assigned a score, which represents the degree of the window belonging to a certain ship category. The ATF can be adaptively obtained by the weighted Logistic Regression (WLR) according to the distribution of backgrounds and targets of the input image. The main innovation of our method is that we only need to collect positive training samples to build the filter, while the negative training samples are adaptively generated by the input image. This is different to other classification method such as Support Vector Machine (SVM) and Logistic Regression (LR), which need to collect both positive and negative training samples. The experimental result on 1-m high resolution optical images shows the proposed method achieves a desired ship detection performance with higher quality and robustness than other methods, e.g., SVM and LR.

  10. An optical NMR spectrometer for Larmor-beat detection and high-resolution POWER NMR

    NASA Astrophysics Data System (ADS)

    Kempf, J. G.; Marohn, J. A.; Carson, P. J.; Shykind, D. A.; Hwang, J. Y.; Miller, M. A.; Weitekamp, D. P.

    2008-06-01

    Optical nuclear magnetic resonance (ONMR) is a powerful probe of electronic properties in III-V semiconductors. Larmor-beat detection (LBD) is a sensitivity optimized, time-domain NMR version of optical detection based on the Hanle effect. Combining LBD ONMR with the line-narrowing method of POWER (perturbations observed with enhanced resolution) NMR further enables atomically detailed views of local electronic features in III-Vs. POWER NMR spectra display the distribution of resonance shifts or line splittings introduced by a perturbation, such as optical excitation or application of an electric field, that is synchronized with a NMR multiple-pulse time-suspension sequence. Meanwhile, ONMR provides the requisite sensitivity and spatial selectivity to isolate local signals within macroscopic samples. Optical NMR, LBD, and the POWER method each introduce unique demands on instrumentation. Here, we detail the design and implementation of our system, including cryogenic, optical, and radio-frequency components. The result is a flexible, low-cost system with important applications in semiconductor electronics and spin physics. We also demonstrate the performance of our systems with high-resolution ONMR spectra of an epitaxial AlGaAs /GaAs heterojunction. NMR linewidths down to 4.1Hz full width at half maximum were obtained, a 103-fold resolution enhancement relative any previous optically detected NMR experiment.

  11. High-resolution speleothem record of precipitation from the Yucatan Peninsula spanning the Maya Preclassic Period

    NASA Astrophysics Data System (ADS)

    Medina-Elizalde, Martín; Burns, Stephen J.; Polanco-Martínez, Josué M.; Beach, Timothy; Lases-Hernández, Fernanda; Shen, Chuan-Chou; Wang, Hao-Cheng

    2016-03-01

    We produced a new high-resolution absolute U-Th dated stalagmite oxygen isotope record (δ18O) from Río Secreto, Playa del Carmen, Yucatan Peninsula (YP). This new 1434-year stalagmite record (named Itzamna after the Maya god of creation) spans the time interval between BCE 1037 and CE 397 with an average resolution of 8 ± 2 years. It provides a novel view of climate evolution over the Preclassic and early Classic periods in Maya history. To understand the controls of regional precipitation δ18O on seasonal time scales, we characterized the amount effect between precipitation amount (P) and precipitation δ18O (δP). We found that precipitation δ18O in the Yucatan Peninsula is controlled by the amount effect on seasonal scales (δP/ΔP = - 0.0137 ± 0.0031‰ per mm, r = 0.9), as suspected but never before demonstrated. Cave drip δ18O is consistent with the annual amount-weighted δ18O composition of precipitation. Multiple lines of evidence suggest that stalagmite δ18O reflects isotopic equilibrium conditions and thus stalagmite δ18O changes are interpreted to reflect precipitation amount. We determined quantitative precipitation changes from the stalagmite δ18O record following previous methods (Medina-Elizalde and Rohling, 2012). The stalagmite precipitation record suggests twelve periods of anomalous precipitation reductions ranging between about 30 and 70% below mean conditions at the time and with durations from 6 years to 31 years. Between BCE 520 and 166, the speleothem precipitation record suggests that the YP experienced an interval of high precipitation labeled the Late Preclassic Humid Period (LPHP) with precipitation maxima of up to + 86 ± 20%. Preclassic Maya cultural expansion in El Mirador Basin, located in northern Guatemala, took place while the peninsula transitioned from the LPHP to an interval with below average precipitation. We find that the Preclassic abandonment of major centers in the Mirador Basin and others around the Maya

  12. Optical contrast enhancement of high-resolution ocular fundus imaging in vivo using polarimetry

    NASA Astrophysics Data System (ADS)

    Yang, Hansheng; Rao, Xuejun; Zhang, Yudong

    2007-11-01

    The adaptive optics (AO) retina imaging was performed with contrast enhancement by characterizing polarization parameters of the living retina. A removable pair of polarization state generating unit near the optical source and analysis unit near the CCD camera was incorporated into the basic 37-channle deformable mirror AO microscopic ophthalmoscope. Double-pass imaging polarimetry of the human eye was carried out, then incomplete Mueller matrix was calculated and analyzed to optimize the retina imaging condition using polarized light, which caused the subretinal structures with different polarization properties to emerge from the scattering light background, so the contrast of the image can be substantially enhanced. This method is demonstrated briefly and its validity was tested in the laboratory. The high-resolution images of ocular fundus are compared with 8-frame-averaging images we obtained prior to this method. The experiment results now show improved visualization of fundus structures to some extent without greatly sacrificing image resolution.

  13. High resolution Holocene sea ice records from Herald Canyon, Chukchi Sea

    NASA Astrophysics Data System (ADS)

    Pearce, Christof; Jakobsson, Martin; O'Regan, Matt; Rattray, Jayne; Barrientos, Natalia; Muchitiello, Francesco; Smittenburg, Rienk; Cronin, Tom; Coxall, Helen; Semiletov, Igor

    2016-04-01

    Arctic Ocean sea ice plays a critical role in the Earth's climate system because of the positive ice-albedo feedback mechanisms as well as its control on ocean-atmospheric heat exchange and potential influence on the thermohaline circulation. Key to improving our understanding of Arctic sea ice cover and its reaction to external forcing is the reconstruction of past variability through paleo-records such as marine sediment cores. Although the observed recent sea ice loss seems to be the strongest of the last millennia, it is still uncertain whether the shift from perennial to seasonal ice cover expected for the near future was unprecedented during the current interglacial. High resolution sea ice reconstructions from the Arctic Ocean are rare, and specifically records from the Russian Arctic are underrepresented. In this study, we present results from marine sediment cores from the Herald Canyon in the East Siberian Sea. The area is one of the major conduits of Pacific water entering the Arctic Ocean basin from the Bering Strait and is thus an ideal place to study past variability of the inflow of these nutrient rich waters. Radiocarbon dating of mollusks indicates very high sedimentation rates at the coring sites which allowed for analyses at centennial resolution up to decadal resolution in the late Holocene. Core samples were analyzed for the biomarker IP25, which is produced by diatoms living in sea ice and is used as a proxy of past seasonal sea ice concentrations. Preliminary results indicate the presence of seasonal sea ice during the entire Late Holocene and show a significant increase of sea ice concentrations during the last millennia.

  14. High resolution Holocene sea ice records from Herald Canyon, East Siberian Sea

    NASA Astrophysics Data System (ADS)

    Pearce, C.; Rattray, J.; Jakobsson, M.; Barrientos, N.; Muschitiello, F.; Smittenberg, R.; O'Regan, M.; Coxall, H.

    2015-12-01

    Arctic Ocean sea ice plays a critical role in the Earth's climate system because of the positive ice-albedo feedback mechanisms as well as its control on ocean-atmospheric heat exchange and potential influence on the thermohaline circulation. Key to improving our understanding of Arctic sea ice cover and its reaction to external forcing is the reconstruction of past variability through paleo-records such as marine sediment cores. Although the observed recent sea ice loss seems to be the strongest of the last millennia, it is still uncertain whether the shift from perennial to seasonal ice cover expected for the near future was unprecedented during the current interglacial. High resolution sea ice reconstructions from the Arctic Ocean are rare, and specifically records from the Russian Arctic are underrepresented. In this study, we present results from marine sediment cores from the Herald Canyon in the East Siberian Sea. The area is one of the major conduits of Pacific water entering the Arctic Ocean basin from the Bering Strait and is thus an ideal place to study past variability of the inflow of these nutrient rich waters. Radiocarbon dating of mollusks indicates very high sedimentation rates at the coring sites which allowed for analyses at centennial resolution up to decadal resolution in the late Holocene. Core samples were analyzed for the biomarker IP25, which is produced by diatoms living in sea ice and is used as a proxy of past seasonal sea ice concentrations. Preliminary results indicate the presence of seasonal sea ice during the entire Late Holocene and show a significant increase of sea ice concentrations during the last millennia.

  15. High resolution Holocene paleomagnetic secular variation records from Bay of Bengal

    NASA Astrophysics Data System (ADS)

    Usapkar, A.; Dewangan, P.; Badesab, F. K.; Mazumdar, A.; Ramprasad, T.; Krishna, K. S.; Basavaiah, N.

    2016-03-01

    We present high resolution paleosecular variation (PSV) records up to 8 cal. kyr BP from three piston cores, MD161/8, MD161/11 and MD161/13 acquired in the Krishna-Godavari (KG) basin, Bay of Bengal. During the Holocene period, high sedimentation rates are recorded at MD161/8 (38.8-248.3 cm/kyr), MD161/11 (137-336 cm/kyr) and MD161/13 (∼573 cm/kyr). Rock magnetic data analysis suggests that the remanence signal is carried by titanomagnetite/titanohematite grains in stable single domain (SSD)/pseudo single domain (PSD) state. The PSV records of MD161/11 and MD161/13 show good correlation in the uppermost sediments despite significant variation in the sedimentation rates; however, poor correlation of PSV records is observed for the core MD161/8 probably due to local effects. Paleoinclination records of MD161/8, MD161/11 and MD161/13 show a low between ∼2.4 and 2.0 cal. kyr BP, an increase between 2.0 and 1.4 cal. kyr BP and a decrease towards the present. To varying degrees these trends can be observed in the other Asian PSV records of Shuangchiling (SCL) and Biwa lakes. However, the magnitude of the observed inclination anomaly in KG basin is higher (∼40°) compared to those reported from SCL (∼25°) and Biwa (∼10°) lakes. Paleodeclination records of MD161/11 and MD161/13 show a decline between ∼4.0 and 2.9 cal. kyr BP, an increase between 2.9 and 2.1 cal. kyr BP, a substantial decrease between ∼2.1 and 1.0 cal. kyr BP and an increase towards the present. Similar trends can be observed in the other Asian PSV records of SCL and Biwa lakes with a minor age offset of 0.2-0.5 kyr. The available models CALS7k.2 and CALS10k.1 are evaluated for their capability in predicting the inclination and declination anomalies from the Asian regions. The CALS7k.2 model can predict most of the inclination anomalies while the CALS10k.1 is unable to predict many of them. The CALS7k.2 model shows that the observed inclination anomalies can be attributed to the presence of

  16. A high-resolution speleothem record of western equatorial Pacific rainfall: Implications for Holocene ENSO evolution

    NASA Astrophysics Data System (ADS)

    Chen, Sang; Hoffmann, Sharon S.; Lund, David C.; Cobb, Kim M.; Emile-Geay, Julien; Adkins, Jess F.

    2016-05-01

    coupled air-sea dynamics that are sensitive to precessional insolation forcing. Isolating the exact mechanisms that drive long-term ENSO evolution will require additional high-resolution paleoclimatic reconstructions and further investigation of Holocene tropical climate evolution using coupled climate models.

  17. A New High-Resolution Record of the Blake Geomagnetic Excursion from ODP Site 1062

    NASA Astrophysics Data System (ADS)

    Bourne, M. D.; Mac Niocaill, C.; Henderson, G. M.; Thomas, A. L.; Faurschou Knudsen, M.

    2010-12-01

    We present a high resolution record of the Blake geomagnetic excursion from Ocean Drilling Program (ODP) Site 1062 on the Blake-Bahama Outer Ridge. The excursion is recorded in three separate cores, with the high sedimentation rate (~10 cm/ka) at this location allowing the determination of transitional field behaviour during the excursion. A complex geometry is observed for the excursional geomagnetic field at the site. The directional records show an initial deviation from the expected directions across an interval of 1 m that achieves a completely reversed state, and then returns to normal polarity. A second, although less well-defined, short-lived phase of anomalous directions is observed immediately following the first event in two of the three cores. Measurements of the magnetic susceptibility show little variation through the core indicating that the concentration and grain size of the remanence carriers remain relatively constant during the studied interval. Measurements of the S-Ratio and remanence coercivity also remain constant through the sections of interest, and indicate magnetite to be the primary remanence carrier. The relatively homogeneous sediment enables the determination of two relative palaeointensity proxies by normalizing natural remanent magnetization measurements using artificially induced magnetizations (anhysteretic remanence, ARM and isothermal remanence, IRM). These records are consistent between all three cores. The relative palaeointensity proxies suggest that the Earth's magnetic field decreased substantially in intensity several tens of kyr prior to the initial event, before reaching an intensity minimum coinciding with the directional excursion maximum. A second palaeointensity minimum is also observed after the excursional event with no associated directional change. These features are consistent with global palaeointensity stacks. Our age model uses a new oxygen isotope stratigraphy. However, rather than assuming a constant

  18. High-resolution in-depth imaging of optically cleared thick samples using an adaptive SPIM

    PubMed Central

    Masson, Aurore; Escande, Paul; Frongia, Céline; Clouvel, Grégory; Ducommun, Bernard; Lorenzo, Corinne

    2015-01-01

    Today, Light Sheet Fluorescence Microscopy (LSFM) makes it possible to image fluorescent samples through depths of several hundreds of microns. However, LSFM also suffers from scattering, absorption and optical aberrations. Spatial variations in the refractive index inside the samples cause major changes to the light path resulting in loss of signal and contrast in the deepest regions, thus impairing in-depth imaging capability. These effects are particularly marked when inhomogeneous, complex biological samples are under study. Recently, chemical treatments have been developed to render a sample transparent by homogenizing its refractive index (RI), consequently enabling a reduction of scattering phenomena and a simplification of optical aberration patterns. One drawback of these methods is that the resulting RI of cleared samples does not match the working RI medium generally used for LSFM lenses. This RI mismatch leads to the presence of low-order aberrations and therefore to a significant degradation of image quality. In this paper, we introduce an original optical-chemical combined method based on an adaptive SPIM and a water-based clearing protocol enabling compensation for aberrations arising from RI mismatches induced by optical clearing methods and acquisition of high-resolution in-depth images of optically cleared complex thick samples such as Multi-Cellular Tumour Spheroids. PMID:26576666

  19. High-resolution in-depth imaging of optically cleared thick samples using an adaptive SPIM

    NASA Astrophysics Data System (ADS)

    Masson, Aurore; Escande, Paul; Frongia, Céline; Clouvel, Grégory; Ducommun, Bernard; Lorenzo, Corinne

    2015-11-01

    Today, Light Sheet Fluorescence Microscopy (LSFM) makes it possible to image fluorescent samples through depths of several hundreds of microns. However, LSFM also suffers from scattering, absorption and optical aberrations. Spatial variations in the refractive index inside the samples cause major changes to the light path resulting in loss of signal and contrast in the deepest regions, thus impairing in-depth imaging capability. These effects are particularly marked when inhomogeneous, complex biological samples are under study. Recently, chemical treatments have been developed to render a sample transparent by homogenizing its refractive index (RI), consequently enabling a reduction of scattering phenomena and a simplification of optical aberration patterns. One drawback of these methods is that the resulting RI of cleared samples does not match the working RI medium generally used for LSFM lenses. This RI mismatch leads to the presence of low-order aberrations and therefore to a significant degradation of image quality. In this paper, we introduce an original optical-chemical combined method based on an adaptive SPIM and a water-based clearing protocol enabling compensation for aberrations arising from RI mismatches induced by optical clearing methods and acquisition of high-resolution in-depth images of optically cleared complex thick samples such as Multi-Cellular Tumour Spheroids.

  20. High-resolution palaeomagnetic records of the Laschamp geomagnetic excursion from the Blake Ridge

    NASA Astrophysics Data System (ADS)

    Mac Niocaill, C.; Bourne, M. D.; Thomas, A. L.; Henderson, G. M.

    2013-05-01

    Geomagnetic excursions are brief (1000s of years) deviations in geomagnetic field behaviour from that expected during 'normal secular' variation. The Laschamp excursion (~41 ka) was a global deviation in geomagnetic field behaviour. Previously published records suggest rapid changes in field direction and a concurrent substantial decrease in field intensity. Accurate dating of excursions and determinations of their durations from multiple locations is vital to our understanding to global field behaviour during these deviations. We present here high-resolution palaeomagnetic records of the Laschamp excursion obtained from two Ocean Drilling Program (ODP) Sites 1061 and 1062 on the Blake-Bahama Outer Ridge (ODP Leg 172) Relatively high sedimentation rates (~30-40 cm kyr-1) at these locations allow the determination of transitional field behaviour during the excursion. Despite their advantages, sedimentary records can be limited by the potential for unrecognized variations in sedimentation rates between widely spaced age-constrained boundaries. Rather than assuming a constant sedimentation rate between assigned age tie-points, we employ measurements of the concentration of 230Thxs in the sediment. 230Thxs is a constant flux proxy and may be used to assess variations in the sedimentation rates through the core sections of interest. Following this approach, we present a new age model for Site 1061 that allows us to better determine the temporal behaviour of the Laschamp excursion with greater accuracy and known uncertainty. Palaeomagnetic measurements of discrete samples from four cores reveal a single excursional feature, across an interval of 30 cm, associated with a broader palaeointensity low. The excursion is characterised by rapid transitions (less than 200 years) between a stable normal polarity and a partially-reversed, polarity. Peaks in inclination either side of the directional excursion indicate periods of time when the local field is dominated by vertical

  1. Development of a flexible optical fiber based high resolution integrated PET/MRI system

    SciTech Connect

    Yamamoto, Seiichi; Watabe, Hiroshi; Kanai, Yasukazu; Watabe, Tadashi; Aoki, Masaaki; Sugiyama, Eiji; Kato, Katsuhiko; Hatazawa, Jun

    2012-11-15

    Purpose: The simultaneous measurement of PET and magnetic resonance imaging (MRI) is an emerging field for molecular imaging research. Although optical fiber based PET/MRI systems have advantages on less interference between PET and MRI, there is a drawback in reducing the scintillation light due to the fiber. To reduce the problem, the authors newly developed flexible optical fiber bundle based block detectors and employed them for a high resolution integrated PET/MRI system. Methods: The flexible optical fiber bundle used 0.5 mm diameter, 80 cm long double clad fibers which have dual 12 mm Multiplication-Sign 24 mm rectangular inputs and a single 24 mm Multiplication-Sign 24 mm rectangular output. In the input surface, LGSO scintillators of 0.025 mol.% (decay time: {approx}31 ns: 0.9 mm Multiplication-Sign 1.3 mm Multiplication-Sign 5 mm) and 0.75 mol.% (decay time: {approx}46 ns: 0.9 mm Multiplication-Sign 1.3 mm Multiplication-Sign 6 mm) were optically coupled in depth direction to form depth-of-interaction detector, arranged in 11 Multiplication-Sign 13 matrix and optically coupled to the fiber bundle. The two inputs of the bundle are bent for 90 Degree-Sign , bound to one, and are optically coupled to a Hamamatsu 1-in. square position sensitive photomultiplier tube. Results: Light loss due to the fiber bundle could be reduced and the performance of the block detectors was improved. Eight optical fiber based block detectors (16 LGSO blocks) were arranged in a 56 mm diameter ring to form a PET system. Spatial resolution and sensitivity were 1.2 mm full-width at half-maximum and 1.2% at the central field-of-view, respectively. Sensitivity change was less than 1% for 2 Degree-Sign C temperature changes. This PET system was integrated with a 0.3 T permanent magnet MRI system which has 17 cm diameter hole at the yoke area for insertion of the PET detector ring. There was no observable interference between PET and MRI. Simultaneous imaging of PET and MRI was

  2. Multimodal adaptive optics for depth-enhanced high-resolution ophthalmic imaging

    NASA Astrophysics Data System (ADS)

    Hammer, Daniel X.; Mujat, Mircea; Iftimia, Nicusor V.; Lue, Niyom; Ferguson, R. Daniel

    2010-02-01

    We developed a multimodal adaptive optics (AO) retinal imager for diagnosis of retinal diseases, including glaucoma, diabetic retinopathy (DR), age-related macular degeneration (AMD), and retinitis pigmentosa (RP). The development represents the first ever high performance AO system constructed that combines AO-corrected scanning laser ophthalmoscopy (SLO) and swept source Fourier domain optical coherence tomography (SSOCT) imaging modes in a single compact clinical prototype platform. The SSOCT channel operates at a wavelength of 1 μm for increased penetration and visualization of the choriocapillaris and choroid, sites of major disease activity for DR and wet AMD. The system is designed to operate on a broad clinical population with a dual deformable mirror (DM) configuration that allows simultaneous low- and high-order aberration correction. The system also includes a wide field line scanning ophthalmoscope (LSO) for initial screening, target identification, and global orientation; an integrated retinal tracker (RT) to stabilize the SLO, OCT, and LSO imaging fields in the presence of rotational eye motion; and a high-resolution LCD-based fixation target for presentation to the subject of stimuli and other visual cues. The system was tested in a limited number of human subjects without retinal disease for performance optimization and validation. The system was able to resolve and quantify cone photoreceptors across the macula to within ~0.5 deg (~100-150 μm) of the fovea, image and delineate ten retinal layers, and penetrate to resolve targets deep into the choroid. In addition to instrument hardware development, analysis algorithms were developed for efficient information extraction from clinical imaging sessions, with functionality including automated image registration, photoreceptor counting, strip and montage stitching, and segmentation. The system provides clinicians and researchers with high-resolution, high performance adaptive optics imaging to help

  3. Ultra-high resolution Fourier domain optical coherence tomography for old master paintings.

    PubMed

    Cheung, C S; Spring, M; Liang, H

    2015-04-20

    In the last 10 years, Optical Coherence Tomography (OCT) has been successfully applied to art conservation, history and archaeology. OCT has the potential to become a routine non-invasive tool in museums allowing cross-section imaging anywhere on an intact object where there are no other methods of obtaining subsurface information. While current commercial OCTs have shown potential in this field, they are still limited in depth resolution (> 4 μm in paint and varnish) compared to conventional microscopic examination of sampled paint cross-sections (~1 μm). An ultra-high resolution fiber-based Fourier domain optical coherence tomography system with a constant axial resolution of 1.2 μm in varnish or paint throughout a depth range of 1.5 mm has been developed. While Fourier domain OCT of similar resolution has been demonstrated recently, the sensitivity roll-off of some of these systems are still significant. In contrast, this current system achieved a sensitivity roll-off that is less than 2 dB over a 1.2 mm depth range with an incident power of ~1 mW on the sample. The high resolution and sensitivity of the system makes it convenient to image thin varnish and glaze layers with unprecedented contrast. The non-invasive 'virtual' cross-section images obtained with the system show the thin varnish layers with similar resolution in the depth direction but superior clarity in the layer interfaces when compared with conventional optical microscope images of actual paint sample cross-sections obtained micro-destructively. PMID:25969057

  4. High Resolution Environmental Magnetic Study of a Holocene Sedimentary Record from Zaca Lake, Ca

    NASA Astrophysics Data System (ADS)

    Platzman, E. S.; Lund, S.; Kirby, M. E.; Feakins, S. J.

    2012-12-01

    Magnetic studies of Holocene lake sediments recovered from Zaca lake have yielded a 3000-year high resolution record of environmental variability and paleolimnology. Zaca lake is a small oligomictic lake ~12m deep situated 730 m above sea level in the steep canyons of the San Rafael mountains, NW of Santa Barbara. Throughout much of the year Zaca lake is anaerobic below 7m. Hydrogen sulfide, fed into the lake via runoff and local sulphur springs, is present throughout the hypolimnion with concentrations sometime exceeding 30 mg/ l. During the summer months when the lake is stratified, light colored carbonate rich microlaminae are formed; and often during the winter months when the lake overturns, killing the anaerobic bacteria, black microlamina rich in iron sulfide are deposited on the lake floor, creating a stratigraphy reflecting patterns of environmental variability on annual to millennial scales. Samples for magnetic analysis were obtained from 8.5 m of core recovered from the central region of Zaca lake. Ages, constrained using radiocarbon chronostratigraphy, yielded sedimentation rates of 2-10 mm/yr with an average rate of 3 mm per yr over the 3000 yr interval. Parameters reflecting decadal scale variability in magnetic concentration (susceptibility, ARM, SIRM) and grainsize (ARM/Chi) were measured every 2 cm. Additional rock magnetic tests, including thermal demagnetization of three component IRM, were applied at selected intervals to constrain the magnetic mineralogy. These data were combined with analyses of clastic grain size, % calcium carbonate and % organics to create a multiproxy record of environmental variability. Results show that Zaca lake has had a complex depositional history. Anthropogenic effects associated with European colonization are present in the upper meters. Most notable, however, is a dramatic shift in the magnetic parameters and mineralogy between the upper and lower half of the core (circa 1300 ybp) indicating a shift in regime

  5. First Complete High-Resolution Record of the Cretaceous Normal Superchron (Invited)

    NASA Astrophysics Data System (ADS)

    Dyment, J.; Gallet, Y.; Hoise, E.

    2009-12-01

    The geomagnetic field behaviour during the Cretaceous Normal Superchron (CNS) remains poorly known, in particular on two points: (1) whether the CNS is really a period of constant, or instead of dominant normal polarity, i.e. if any short reversed polarity interval exists within the CNS, as some magnetostratigraphy works have suggested the presence of at least one short event named ISEA; (2) whether the geomagnetic intensity during the CNS is higher, lower, displays stronger variations, or exhibits a behaviour similar to the present-day field, as contradictory results have emerged from studies of individual volcanic samples, sedimentary and volcanic sections, and sea-surface magnetic anomalies. In this paper, we present the first complete high-resolution record of the CNS. This record was collected during cruise Magofond 08 of R/V Le Suroît in the Central Atlantic Ocean, using a deep-tow marine magnetometer towed 500 to 1000 m above the seafloor across anomalies M4-M0, the CNS, anomaly 33r and a part of anomaly 33. The 900 km-long deep-tow profile was acquired at a speed of 1.5 knot during not less than 14 days, along a flowline of the Mid-Atlantic Ridge on the African flank, west of the Canary Islands, at ~26-29°N. Seismic data along the same profile collected during a previous cruise have been used to remove the effect of the basement topography. Comparison with shorter mid-tow (1000-1500 m altitude) profiles collected 300 to 500 km away in the same area shows a good correlation and some variations related to seafloor spreading processes. The record displays strong and uninterrupted variations of the magnetic anomaly before, during and after the CNS (the wavelength of which ultimately depending on the altitude of the measurement above the basement), suggesting similar variations of the geomagnetic intensity during the whole time span. At least five short wavelength anomalies within the CNS are as strong as or stronger than those associated to known reversals

  6. Mid-infrared high-resolution absorption spectroscopy by use of a semimonolithic entangled-cavity optical parametric oscillator.

    PubMed

    Desormeaux, A; Lefebvre, M; Rosencher, E; Huignard, J P

    2004-12-15

    By recording low-pressure absorption lines of N2O around 3.9 microm, we fully qualify a pulsed entangled-cavity doubly resonant optical parametric oscillator as a power tool for high-resolution spectroscopy. This compact source runs at a high repetition rate (>10 kHz) with a low threshold of oscillation (<8 microJ), is mode-hop-free tunable over 5 cm(-1), and displays single-frequency Fourier-transformed-limited operation (linewidth <0.005 cm(-1)). A high potential for nonlinear spectroscopy is also expected given the high peak power (70 W) and the good quality (M2 < 2) of the output beam. PMID:15645813

  7. High-resolution conodont oxygen isotope record of Ordovician climate change

    NASA Astrophysics Data System (ADS)

    Chen, J.; Chen, Z.; Algeo, T. J.

    2013-12-01

    The Ordovician Period was characterized by several major events, including a prolonged 'super greenhouse' during the Early Ordovician, the 'Great Ordovician Biodiversification Event (GOBE)' of the Middle and early Late Ordovician, and the Hirnantian ice age and mass extinction of the latest Ordovician (Webby et al., 2004, The Great Ordovician Biodiversification Event, Columbia University Press). The cause of the rapid diversification of marine invertebrates during the GOBE is not clear, however, and several scenarios have been proposed including widespread development of shallow cratonic seas, strong magmatic and tectonic activity, and climate moderation. In order to investigate relationships between climate change and marine ecosystem evolution during the Ordovician, we measured the oxygen isotopic composition of single coniform conodonts using a Cameca secondary ion mass spectrometer. Our δ18O profile shows a shift at the Early/Middle Ordovician transition that is indicative of a rapid 6 to 8 °C cooling. This cooling event marks the termination of the Early Ordovician 'super greenhouse' and may have established cooler tropical seawater temperatures that were more favorable for invertebrate animals, setting the stage for the GOBE. Additional cooling episodes occurred during the early Sandbian, early Katian, and Hirnantian, the last culminating in a short-lived (<1-Myr) end-Ordovician ice age. The much cooler conditions that prevailed at that time may have been an important factor in the end-Ordovician mass extinction. Our results differ from those of Trotter et al. (2008, 'Did cooling oceans trigger Ordovician biodiversification? Evidence from conodont thermometry,' Science 321:550-554). Instead of a slow, protracted cooling through the Early and Middle Ordovician, our high-resolution record shows that cooling occurred in several discrete steps, with the largest step being at the Early/Middle Ordovician transition.

  8. The Results of CYG X-1 High-Resolution Optical Spectral Monitoring

    NASA Astrophysics Data System (ADS)

    Karitskaya, Eugenia A.

    2007-08-01

    Selected results of optical high-resolution spectral observations 2002-2004 are briefly reviewed. Optical line profile variations were detected during X-ray flare. The comparison of observed and non-LTE model profiles for HI, HeI and MgII is given taking into account tidal distortion of Cyg X-1 optical component and its illumination by X-ray emission of secondary one. We set limits on the optical component main characteristics Teff = 30400±500K, log g = 3.31±0.07 and overabundance of He and C, N, O, Mg, Al, Si, S, Fe and Zn by using spectra of 2003-2004. The Doppler images were reconstructed by an improved Doppler tomography method on the base of HeII4686A profiles of 2003 (“soft” X-ray state) and 2004 (“hard” X-ray state). It allowed putting a limitation on the black hole to supergiant mass ratio 1/4 < M/MO < 1/3. The photometric and spectral variations point to the supergiant parameters changes on the time scale of tens of years. Line profile non-LTE simulations lead to the conclusion that the star radius has grown about 1-4% from 1997 to 2003-2004 and the temperature decreased by 1300-2400K.

  9. High-resolution dual-trap optical tweezers with differential detection: alignment of instrument components.

    PubMed

    Bustamante, Carlos; Chemla, Yann R; Moffitt, Jeffrey R

    2009-10-01

    Optical traps or "optical tweezers" have become an indispensable tool in understanding fundamental biological processes. Using our design, a dual-trap optical tweezers with differential detection, we can detect length changes to a DNA molecule tethering the trapped beads of 1 bp. By forming two traps from the same laser and maximizing the common optical paths of the two trapping beams, we decouple the instrument from many sources of environmental and instrumental noise that typically limit spatial resolution. The performance of a high-resolution instrument--the formation of strong traps, the minimization of background signals from trap movements, or the mitigation of the axial coupling, for example--can be greatly improved through careful alignment. This procedure, which is described in this article, starts from the laser and advances through the instrument, component by component. Alignment is complicated by the fact that the trapping light is in the near infrared (NIR) spectrum. Standard infrared viewing cards are commonly used to locate the beam, but unfortunately, bleach quickly. As an alternative, we use an IR-viewing charge-coupled device (CCD) camera equipped with a C-mount telephoto lens and display its image on a monitor. By visualizing the scattered light on a pair of irises of identical height separated by >12 in., the beam direction can be set very accurately along a fixed axis. PMID:20147041

  10. A New High-Resolution Record of the Blake Geomagnetic Excursion from ODP Site 1062

    NASA Astrophysics Data System (ADS)

    Bourne, Mark; Mac Niocaill, Conall; Henderson, Gideon; Thomas, Alex; Knudsen, Mads

    2010-05-01

    We present a high resolution record of the Blake geomagnetic excursion from Ocean Drilling Program (ODP) Site 1062 on the Blake-Bahama Outer Ridge. The excursion is recorded in three separate cores, with the high average sedimentation rate (10 cm ka-1) at this location allowing the determination of transitional field behaviour during the excursion. A complex geometry is observed for the excursional geomagnetic field. The directional records show an initial deviation from the expected directions across an interval of 1 m that achieves a completely reversed state, and then returns to normal polarity. A second, although less well-defined, short-lived phase of anomalous directions is observed immediately following the first event in two of the three cores. Measurements of the magnetic susceptibility show little variation through the core indicating that the concentration and grain size of the remanence carriers remains relatively constant throughout the studied interval. Measurements of the S-Ratio and remanence coercivity also remain constant through the core sections of interest, and indicate magnetite to be the primary remanence carrier. The relatively homogeneous sediment enables the determination of two relative palaeointensity proxies by normalizing natural remanent magnetization measurements using artificially induced magnetizations (anhysteretic remanence, ARM and isothermal remanence, IRM). These records are consistent between all three cores. The relative palaeointensity proxies suggest that the Earth's magnetic field decreased substantially in intensity up to 70 ka prior to the initial event, before reaching an intensity minimum coinciding with the directional excursion maximum. A second palaeointensity minimum is also observed after the excursional event with no associated directional change. These features are consistent with global palaeointensity stacks. A preliminary age model based on an oxygen isotope stratigraphy, and an average sedimentation rate

  11. Optical coherence tomography for high-resolution imaging of mouse development in utero

    NASA Astrophysics Data System (ADS)

    Syed, Saba H.; Larin, Kirill V.; Dickinson, Mary E.; Larina, Irina V.

    2011-04-01

    Although the mouse is a superior model to study mammalian embryonic development, high-resolution live dynamic visualization of mouse embryos remain a technical challenge. We present optical coherence tomography as a novel methodology for live imaging of mouse embryos through the uterine wall thereby allowing for time lapse analysis of developmental processes and direct phenotypic analysis of developing embryos. We assessed the capability of the proposed methodology to visualize structures of the living embryo from embryonic stages 12.5 to 18.5 days postcoitus. Repetitive in utero embryonic imaging is demonstrated. Our work opens the door for a wide range of live, in utero embryonic studies to screen for mutations and understand the effects of pharmacological and toxicological agents leading to birth defects.

  12. “Ultra-high resolution optical trap with single fluorophore sensitivity”

    PubMed Central

    Comstock, Matthew J; Ha, Taekjip; Chemla, Yann R

    2013-01-01

    We present a single-molecule instrument that combines a timeshared ultra-high resolution dual optical trap interlaced with a confocal fluorescence microscope. In a demonstration experiment, individual single-fluorophore labeled DNA oligonucleotides were observed to bind and unbind to complementary DNA suspended between two trapped beads. Simultaneous with the single-fluorophore detection, coincident angstrom-scale changes in tether extension could be clearly observed. Fluorescence readout allowed us to determine the duplex melting rate as a function of force. The new instrument will enable the simultaneous measurement of angstrom-scale mechanical motion of individual DNA-binding proteins (e.g., single base pair stepping of DNA translocases) along with the detection of fluorescently labeled protein properties (e.g., internal configuration). PMID:21336286

  13. High-resolution refractive index anisotropy measurement in optical fibers through phase retardation modulation

    NASA Astrophysics Data System (ADS)

    Sévigny, Benoit; Busque, François; Godbout, Nicolas; Lacroix, Suzanne; Faucher, Mathieu

    2008-03-01

    We present an improved, high-resolution method for the measurement of phase retardation induced by the material birefringence of optical fibers. Such a method can be used to retrieve information about the spatial distribution of refractive index anisotropy in the fiber by comparing the accumulated phase of a polarization component oriented along the fiber transmission axis and another located in the transverse plane. The method is based on the nonlinear regression of a phase modulated signal of known modulation amplitude altered by the sample. Experimental results obtained with our method for a standard telecommunications fiber (the Corning SMF-28) as well as photosensitive fibers (Fibercore PS1250 and PS1500) are presented with a noise-limited phase resolution below 10-4 radians and a spatial resolution below 1 μm. An analysis of the limitation of such measurement methods is also presented including diffraction by the fibers.

  14. Optical coherence tomography for high-resolution imaging of mouse development in utero

    PubMed Central

    Syed, Saba H.; Larin, Kirill V.; Dickinson, Mary E.; Larina, Irina V.

    2011-01-01

    Although the mouse is a superior model to study mammalian embryonic development, high-resolution live dynamic visualization of mouse embryos remain a technical challenge. We present optical coherence tomography as a novel methodology for live imaging of mouse embryos through the uterine wall thereby allowing for time lapse analysis of developmental processes and direct phenotypic analysis of developing embryos. We assessed the capability of the proposed methodology to visualize structures of the living embryo from embryonic stages 12.5 to 18.5 days postcoitus. Repetitive in utero embryonic imaging is demonstrated. Our work opens the door for a wide range of live, in utero embryonic studies to screen for mutations and understand the effects of pharmacological and toxicological agents leading to birth defects. PMID:21529073

  15. A comparison of high resolution optical and radio observations of W3

    NASA Technical Reports Server (NTRS)

    Dickel, H. R.; Harten, R. H.; Gull, T. R.

    1983-01-01

    High resolution maps of the W3 complex for radio continuum and selected optical emission lines are used to derive the visual extinction A(V), the excitation, and the evolutionary state of the component H II regions. A large diffuse H II region in the east, which is the most highly evolved of this type, is found, together with a southern complex of H II regions and a northern complex of young and compact H II regions still embedded in the W3 molecular core. A comparison of the observed dust distribution with ionized and atomic hydrogen and several other molecules in W3 shows the functional relationship between the C-130 column density and A(V) to be in accord with Strohacker's (1978) result for dark clouds.

  16. A low-cost, high-resolution, video-rate imaging optical radar

    SciTech Connect

    Sackos, J.T.; Nellums, R.O.; Lebien, S.M.; Diegert, C.F.; Grantham, J.W.; Monson, T.

    1998-04-01

    Sandia National Laboratories has developed a unique type of portable low-cost range imaging optical radar (laser radar or LADAR). This innovative sensor is comprised of an active floodlight scene illuminator and an image intensified CCD camera receiver. It is a solid-state device (no moving parts) that offers significant size, performance, reliability, and simplicity advantages over other types of 3-D imaging sensors. This unique flash LADAR is based on low cost, commercially available hardware, and is well suited for many government and commercial uses. This paper presents an update of Sandia`s development of the Scannerless Range Imager technology and applications, and discusses the progress that has been made in evolving the sensor into a compact, low, cost, high-resolution, video rate Laser Dynamic Range Imager.

  17. High resolution record of the Last Glacial Maximum in eastern Australia

    NASA Astrophysics Data System (ADS)

    Petherick, Lynda; Moss, Patrick; McGowan, Hamish

    2010-05-01

    A continuous, high resolution (average ca. 22 year) record encompassing the Last Glacial Maximum (LGM) has been developed using multiple proxies (aeolian sediment flux, grain size, pollen and charcoal) in lake sediment from Tortoise Lagoon (TOR), North Stradbroke Island, Queensland, Australia. The presence of Asteraceae tubilifloreae and spineless Asteraceae (common indicators of glacial conditions in Australia) at TOR indicates significantly cooler temperatures (mean annual temperature up to 6oC lower than today). In addition to the palaeoclimatic reconstruction, a record of palaeodust transport pathways for eastern Australia was developed using ICP-MS trace element analysis and geochemical "fingerprinting" of TOR aeolian sediment to continental dust source areas. Vectors between dominant dust source areas and North Stradbroke Island allowed the reconstruction of the position and intensity of LGM dust transport pathways. Furthermore, changes in likely synpotic scale conditions can be postulated based on the position of the dust transport corridors. Similarities between the vegetation at TOR during the LGM and that at temperate sites e.g. Caledonia Fen, Victoria (Kershaw et al. 2007), Redhead Lagoon, New South Wales (Williams et al. 2006) and Barrington Tops, New South Wales (Sweller and Martin 2001) suggests that this record reflects regional conditions across southeastern Australia. The TOR record also correlates well with that from nearby Native Companion Lagoon which suggests that the LGM was actually an extended period of ca. 8 - 10 kyr, characterised by 2 periods of increased aridity (ca. 30 - 26.5 kyr and 21 - 19.5 kyr) (Petherick et al. 2008). A growing number of records from across the Southern Hemisphere e.g. New Zealand (Suggate and Almond 2003; Alloway et al. 2007; Newnham et al. 2007), Chile (Denton et al. 1999), Antarctica (Röthlisberger et al. 2002; EPICA 2006) and Australia (Smith 2009) also show evidence that the LGM encompassed a longer period of

  18. Quaternary stratigraphy of Bermuda: A high-resolution pre-Sangamonian rock record

    NASA Astrophysics Data System (ADS)

    Hearty, Paul J.; Vacher, H. Leonard

    Carbonate islands such as Bermuda are created by climatic change. Warm climates and high sea levels stimulate carbonate sediment production that may ultimately result in island growth, while cold glacials expose the platforms to weathering, dissolution and soil formation. Of great importance in Quaternary studies is the ability to decipher this climatic history. Mapping and geochronologic studies have established that Bermuda may have one of the most continuous and detailed Quaternary interglacial depositional records on a carbonate platform. Advances in racemization dating (AAR) have offered a means of deciphering this climatic history and generating a high-resolution stratigraphic and age framework for the Quaternary. Bermudian interglacial units consist predominantly of eolianites, with less voluminous occurrences of beach deposits and calcarenite protosols (Entisols). Glacial or stadial-age terra rossa (aluminous laterite) paleosols, whose degree of development is a function of time of exposure, form boundaries between interglacial units. D-alloiso-leucine/ L-isoleucine ( {A}/{I}) ratios have been determined on marine pelecypods, land snails and whole-rock samples from mapped sections; aminozones have been defined for two Sangamonian and at least five pre-Sangamonian depositional intervals. From kinetic models based on calibration with previously published U-series coral dates, estimated ages of middle Pleistocene and older aminozones are: F = 190,000-265,000 years; G = 300,000-400,000 years; H = 400,000-500,000 years; J = >700,000 years; and K = > 900,000 years. Aminozone G, which is correlated with the upper Town Hill Formation and Isotope Stage 9, is volumetrically the most important depositional event of the middle Pleistocene. The great mass of sediment deposited during this period suggests an interglacial of significant duration and prolonged shelf submergence, during which the island grew to over half its present size. Only the Sangamonian ( sensu lato

  19. High-Resolution Continental Records of Early Paleogene Hyperthermals from the Bighorn Basin Coring Project

    NASA Astrophysics Data System (ADS)

    Wing, S. L.; Clyde, W. C.; Gingerich, P. D.

    2012-12-01

    Between 50 and 60 million years ago the earth experienced several geologically brief and sudden episodes of global warming, called hyperthermals, each associated with a negative carbon isotope excursion indicating a perturbation of the global carbon cycle. Hyperthermals shed light on connections between the carbon cycle and climate that are important for understanding anthropogenic global warming, and as a result have been the subject of intense study, particularly in deep-sea cores. Hyperthermals are less well known in the terrestrial realm. The goal of the Bighorn Basin Coring Project (BBCP) is to produce high-resolution records that reveal changes in climate, landscapes and ecological communities that occurred in the middle of the North American continent during three hyperthermals. In the summer of 2011, BBCP scientists cored fluvial rocks representing the Paleocene-Eocene Thermal Maximum (PETM; ~56 Ma), Eocene Thermal Maximum 2 (ETM2; ~53.7 Ma) and H2 (~53.7 Ma). These cores provide continuous sections of fresh rock in direct stratigraphic superposition that are being analyzed for chemical and physical properties as well as fossils. High depositional rates at all three sites (30-50 cm/thousand years) permit resolution of events on a millenial timescale. The PETM was double cored at two sites, Basin Substation and Polecat Bench, that provide contrasting local environments. Basin Substation sediments were deposited on wet floodplains near the eastern flank of the Bighorn Basin and have higher concentrations of organic microfossils and biomarkers. Early studies of the ~140 m-thick Basin Substation cores, which were drilled with municipal water only, have focused on pollen and spores, n-alkanes, polycyclic aromatic hydrocarbons, GDGTs, plant mesofossils, and the isotopic composition of dispersed organic matter. The Polecat Bench site was closer to the depositional axis of the Bighorn Basin, but floodplain sediments were generally better drained and have higher

  20. Focus Variation - A New Technology for High Resolution Optical 3D Surface Metrology

    NASA Astrophysics Data System (ADS)

    Scherer, S.

    2009-04-01

    Focus Variation - A New Technology for High Resolution Optical 3D Surface Metrology S. Scherer1, E. Cristea1, O. Huber1, A. Krenn1 1 ALICONA GmbH Graz, Austria The need for increasing accuracy is a characteristic of all geo-applications, and hence of the instruments contributing to obtaining relevant data. Small and fine sensors are being developed, measuring different parameters of our geosystem and requiring continuous validation and calibration. These sensors have often very small components (fine sensors able to sense dust, atmospheric water vapour characteristics, pressure change, gravimeters, satellite micro-components), showing complex topographies including steep flanks and having varying reflective properties. In order to get valid and reliable results, quality assurance of these instruments and sensors is required. The optical technology Focus-Variation, developed by Alicona and added in the latest draft of the upcoming ISO standard 25178, provides high resolution 3D surface metrology even at those complex topographies. The technique of Focus-Variation combines the small depth of focus of an optical system with vertical scanning to provide topographical and color information from the variation of focus. It is used for high-resolution optical 3D surface measurements. The traceable and repeatable measurement results are further being used for calibration and validation purposes. Some of the characteristics of the technology are: - Measurement of instruments / samples with steep flanks up to 80° - Measurement of materials with strongly varying reflection properties - Measurement of surfaces presenting fine (from 10nm) or strong roughness Here, we present the operating principle and possible applications of the optical 3D measurement system "InfiniteFocus", which is based on the technology of Focus-Variation and used for quality assurance in the lab and in production. With the vertical resolution of up to 10nm, InfiniteFocus yields meaningful form and

  1. Multi-sensor fusion of infrared and electro-optic signals for high resolution night images.

    PubMed

    Huang, Xiaopeng; Netravali, Ravi; Man, Hong; Lawrence, Victor

    2012-01-01

    Electro-optic (EO) image sensors exhibit the properties of high resolution and low noise level at daytime, but they do not work in dark environments. Infrared (IR) image sensors exhibit poor resolution and cannot separate objects with similar temperature. Therefore, we propose a novel framework of IR image enhancement based on the information (e.g., edge) from EO images, which improves the resolution of IR images and helps us distinguish objects at night. Our framework superimposing/blending the edges of the EO image onto the corresponding transformed IR image improves their resolution. In this framework, we adopt the theoretical point spread function (PSF) proposed by Hardie et al. for the IR image, which has the modulation transfer function (MTF) of a uniform detector array and the incoherent optical transfer function (OTF) of diffraction-limited optics. In addition, we design an inverse filter for the proposed PSF and use it for the IR image transformation. The framework requires four main steps: (1) inverse filter-based IR image transformation; (2) EO image edge detection; (3) registration; and (4) blending/superimposing of the obtained image pair. Simulation results show both blended and superimposed IR images, and demonstrate that blended IR images have better quality over the superimposed images. Additionally, based on the same steps, simulation result shows a blended IR image of better quality when only the original IR image is available. PMID:23112602

  2. Improved fusing infrared and electro-optic signals for high-resolution night images

    NASA Astrophysics Data System (ADS)

    Huang, Xiaopeng; Netravali, Ravi; Man, Hong; Lawrence, Victor

    2012-06-01

    Electro-optic (EO) images exhibit the properties of high resolution and low noise level, while it is a challenge to distinguish objects with infrared (IR), especially for objects with similar temperatures. In earlier work, we proposed a novel framework for IR image enhancement based on the information (e.g., edge) from EO images. Our framework superimposed the detected edges of the EO image with the corresponding transformed IR image. Obviously, this framework resulted in better resolution IR images that help distinguish objects at night. For our IR image system, we used the theoretical point spread function (PSF) proposed by Russell C. Hardie et al., which is composed of the modulation transfer function (MTF) of a uniform detector array and the incoherent optical transfer function (OTF) of diffraction-limited optics. In addition, we designed an inverse filter based on the proposed PSF to transform the IR image. In this paper, blending the detected edge of the EO image with the corresponding transformed IR image and the original IR image is the principal idea for improving the previous framework. This improved framework requires four main steps: (1) inverse filter-based IR image transformation, (2) image edge detection, (3) images registration, and (4) blending of the corresponding images. Simulation results show that blended IR images have better quality over the superimposed images that were generated under the previous framework. Based on the same steps, the simulation result shows a blended IR image of better quality when only the original IR image is available.

  3. Multi-Sensor Fusion of Infrared and Electro-Optic Signals for High Resolution Night Images

    PubMed Central

    Huang, Xiaopeng; Netravali, Ravi; Man, Hong; Lawrence, Victor

    2012-01-01

    Electro-optic (EO) image sensors exhibit the properties of high resolution and low noise level at daytime, but they do not work in dark environments. Infrared (IR) image sensors exhibit poor resolution and cannot separate objects with similar temperature. Therefore, we propose a novel framework of IR image enhancement based on the information (e.g., edge) from EO images, which improves the resolution of IR images and helps us distinguish objects at night. Our framework superimposing/blending the edges of the EO image onto the corresponding transformed IR image improves their resolution. In this framework, we adopt the theoretical point spread function (PSF) proposed by Hardie et al. for the IR image, which has the modulation transfer function (MTF) of a uniform detector array and the incoherent optical transfer function (OTF) of diffraction-limited optics. In addition, we design an inverse filter for the proposed PSF and use it for the IR image transformation. The framework requires four main steps: (1) inverse filter-based IR image transformation; (2) EO image edge detection; (3) registration; and (4) blending/superimposing of the obtained image pair. Simulation results show both blended and superimposed IR images, and demonstrate that blended IR images have better quality over the superimposed images. Additionally, based on the same steps, simulation result shows a blended IR image of better quality when only the original IR image is available. PMID:23112602

  4. Near-Field Scanning Optical Microscopy for High-Resolution Membrane Studies

    PubMed Central

    Huckabay, Heath A.; Armendariz, Kevin P.; Newhart, William H.; Wildgen, Sarah M.; Dunn, Robert C.

    2012-01-01

    The desire to directly probe biological structures on the length scales that they exist has driven the steady development of various high-resolution microscopy techniques. Among these, optical microscopy and, in particular, fluorescence-based approaches continue to occupy dominant roles in biological studies given their favorable attributes. Fluorescence microscopy is both sensitive and specific, is generally noninvasive toward biological samples, has excellent temporal resolution for dynamic studies, and is relatively inexpensive. Light-based microscopies can also exploit a myriad of contrast mechanisms based on spectroscopic signatures, energy transfer, polarization, and lifetimes to further enhance the specificity or information content of a measurement. Historically, however, spatial resolution has been limited to approximately half the wavelength due to the diffraction of light. Near-field scanning optical microscopy (NSOM) is one of several optical approaches currently being developed that combines the favorable attributes of fluorescence microscopy with superior spatial resolution. NSOM is particularly well suited for studies of both model and biological membranes and application to these systems is discussed. PMID:23086886

  5. Aerosol Optical Depth Model Assessment With High-Resolution Multiple Angle Sensors

    NASA Astrophysics Data System (ADS)

    Martin, J. S.; Nielsen, K. E.; Vincent, D. A.; Durkee, P. A.; Reid, J. S.

    2005-12-01

    The Naval Postgraduate School Aerosol Optical Depth (NPS AOD) model has been used successfully to retrieve aerosol optical depths over water using Advanced Very High Resolution Radiometer (AVHRR) imagery. In this work, the NPS AOD model is applied to the QuickBird high-resolution commercial satellite imagery collected at multiple zenith angles around Sir Bu Nuair Island, United Arab Emirates in September 2004 during the Unified Aerosol Experiment, United Arab Emirates (UAE2) Campaign. The QuickBird-retrieved aerosol optical depths are compared to other satellite and ground-based optical depth retrievals, including those from the Aeerosol Robotic NETwork (AERONET), the MODerate resolution Imaging Spectroradiometer (MODIS), Multi-angle Imaging Spectroradiometer (MISR), and AVHRR. Adapting the NPS AOD model to the nominally 2.4-meter resolution imagery from QuickBird required using modal radiances determined over an area that matched the lower resolution imagers (~ 275 meters to 1 kilometer). Additionally, the NPS AOD model was originally developed for the AVHRR imager on the NOAA-14 satellite. The NPS AOD model selects a modeled aerosol size distribution and scattering phase function based on the ratio the red and near-infrared channels of the AVHRR and the scattering angle derived from solar-sensor geometry. As such, the LUT that relates the ratio of red and near-infrared radiances was based on the center effective wavelengths of the NOAA-14 channels. The AOD retrievals from the other imagers must be adjusted to account for the changes in center effective wavelengths of the red and near-IR channels. Results show that the application of the NPS AOD model to QuickBird data yields findings that are consistent with other satellite and ground-based retrievals. In general, the NPS AOD model works well for nadir and near-nadir view angles, but not for zenith angles greater than 50 degrees. A non-linearized single scattering model and additional scattering streams will be

  6. First steps toward 3D high resolution imaging using adaptive optics and full-field optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Blanco, Leonardo; Blavier, Marie; Glanc, Marie; Pouplard, Florence; Tick, Sarah; Maksimovic, Ivan; Chenegros, Guillaume; Mugnier, Laurent; Lacombe, Francois; Rousset, Gérard; Paques, Michel; Le Gargasson, Jean-François; Sahel, Jose-Alain

    2008-09-01

    We describe here two parts of our future 3D fundus camera coupling Adaptive Optics and full-field Optical Coherence Tomography. The first part is an Adaptive Optics flood imager installed at the Quinze-Vingts Hospital, regularly used on healthy and pathological eyes. A posteriori image reconstruction is performed, increasing the final image quality and field of view. The instrument lateral resolution is better than 2 microns. The second part is a full-field Optical Coherence Tomograph, which has demonstrated capability of performing a simple kind of "4 phases" image reconstruction of non biological samples and ex situ retinas. Final aim is to couple both parts in order to achieve 3D high resolution mapping of in vivo retinas.

  7. Simultaneous high-resolution pH and spectrophotometric recordings of oxygen binding in blood microvolumes.

    PubMed

    Oellermann, Michael; Pörtner, Hans-O; Mark, Felix C

    2014-05-01

    Oxygen equilibrium curves have been widely used to understand oxygen transport in numerous organisms. A major challenge has been to monitor oxygen binding characteristics and concomitant pH changes as they occur in vivo, in limited sample volumes. Here we report a technique allowing highly resolved and simultaneous monitoring of pH and blood pigment saturation in minute blood volumes. We equipped a gas diffusion chamber with a broad-range fibre-optic spectrophotometer and a micro-pH optode and recorded changes of pigment oxygenation along oxygen partial pressure (PO2) and pH gradients to test the setup. Oxygen binding parameters derived from measurements in only 15 μl of haemolymph from the cephalopod Octopus vulgaris showed low instrumental error (0.93%) and good agreement with published data. Broad-range spectra, each resolving 2048 data points, provided detailed insight into the complex absorbance characteristics of diverse blood types. After consideration of photobleaching and intrinsic fluorescence, pH optodes yielded accurate recordings and resolved a sigmoidal shift of 0.03 pH units in response to changing PO2 from 0 to 21 kPa. Highly resolved continuous recordings along pH gradients conformed to stepwise measurements at low rates of pH changes. In this study we showed that a diffusion chamber upgraded with a broad-range spectrophotometer and an optical pH sensor accurately characterizes oxygen binding with minimal sample consumption and manipulation. We conclude that the modified diffusion chamber is highly suitable for experimental biologists who demand high flexibility, detailed insight into oxygen binding as well as experimental and biological accuracy combined in a single setup. PMID:24436387

  8. High-resolution apertureless near-field optical imaging using gold nanosphere probes.

    PubMed

    Kim, Zee Hwan; Leone, Stephen R

    2006-10-12

    An apertureless near-field scanning optical microscope (ANSOM) that utilizes the enhanced field around a gold nanosphere, which is attached to the end of an atomic force microscope (AFM) tip, is used to image the local dielectric constant of the patterned metallic surfaces and local electric field around plasmonic nanosphere samples. A colloidal gold nanosphere (approximately 50 nm diameter) is linked to the extremity of the conventional etched-silicon probe. The scattering of laser radiation (633 or 532 nm) is modulated by the oscillating nanosphere-functionalized silicon tip, and the scattered radiation is detected. The approach curve (scattering intensity as a function of the tip-sample distance), the polarization dependence (scattering intensity as a function of the excitation polarization direction), and ANSOM image contrast confirm that the spherical nanosphere attached to the silicon tip acts as a point dipole that interacts with the sample surface via a dipole-dipole coupling, in which the dipole created by the field at the tip interacts with its own image dipole in the sample. The image obtained with the nanoparticle functionalized tip provides a dielectric map of the sample surface with a spatial resolution better than 80 nm. In addition, we show that the functionalized tip is capable of imaging the local electric field distribution above the plasmonic nanosphere samples. Overall, the result shows that high-resolution ANSOM is possible without the aid of the lightning-rod effect. With an improved tip-fabrication method, we believe that the method can provide a versatile high-resolution chemical imaging that is not available from usual forms of ANSOM. PMID:17020365

  9. A new method of inshore ship detection in high-resolution optical remote sensing images

    NASA Astrophysics Data System (ADS)

    Hu, Qifeng; Du, Yaling; Jiang, Yunqiu; Ming, Delie

    2015-10-01

    Ship as an important military target and water transportation, of which the detection has great significance. In the military field, the automatic detection of ships can be used to monitor ship dynamic in the harbor and maritime of enemy, and then analyze the enemy naval power. In civilian field, the automatic detection of ships can be used in monitoring transportation of harbor and illegal behaviors such as illegal fishing, smuggling and pirates, etc. In recent years, research of ship detection is mainly concentrated in three categories: forward-looking infrared images, downward-looking SAR image, and optical remote sensing images with sea background. Little research has been done into ship detection of optical remote sensing images with harbor background, as the gray-scale and texture features of ships are similar to the coast in high-resolution optical remote sensing images. In this paper, we put forward an effective harbor ship target detection method. First of all, in order to overcome the shortage of the traditional difference method in obtaining histogram valley as the segmentation threshold, we propose an iterative histogram valley segmentation method which separates the harbor and ships from the water quite well. Secondly, as landing ships in optical remote sensing images usually lead to discontinuous harbor edges, we use Hough Transform method to extract harbor edges. First, lines are detected by Hough Transform. Then, lines that have similar slope are connected into a new line, thus we access continuous harbor edges. Secondary segmentation on the result of the land-and-sea separation, we eventually get the ships. At last, we calculate the aspect ratio of the ROIs, thereby remove those targets which are not ship. The experiment results show that our method has good robustness and can tolerate a certain degree of noise and occlusion.

  10. High-resolution three-dimensional in vivo imaging of mouse oviduct using optical coherence tomography

    PubMed Central

    Burton, Jason C.; Wang, Shang; Stewart, C. Allison; Behringer, Richard R.; Larina, Irina V.

    2015-01-01

    The understanding of the reproductive events and the molecular mechanisms regulating fertility and infertility in humans relies heavily on the analysis of the corresponding phenotypes in mouse models. While molecular genetic approaches provide significant insight into the molecular regulation of these processes, the lack of live imaging methods that allow for detailed visualization of the mouse reproductive organs limits our investigations of dynamic events taking place during the ovulation, the fertilization and the pre-implantation stages of embryonic development. Here we introduce an in vivo three-dimensional imaging approach for visualizing the mouse oviduct and reproductive events with micro-scale spatial resolution using optical coherence tomography (OCT). This method relies on the natural tissue optical contrast and does not require the application of any contrast agents. For the first time, we present live high-resolution images of the internal structural features of the oviduct, as well as other reproductive organs and the oocytes surrounded by cumulus cells. These results provide the basis for a wide range of live dynamic studies focused on understanding fertility and infertility. PMID:26203393

  11. Ultra-high Resolution Optics for EUV and Soft X-ray Inelastic Scattering

    SciTech Connect

    Voronov, Dmitry L.; Cambie, Rossana; Ahn, Minseung; Anderson, Erik H.; Chang, Chih-Hao; Gullikson, Eric M.; Heilmann, Ralf K.; Salmassi, Farhad; Schattenburg, Mark L.; Yashchuk, Valeriy V.; Padmore, Howard A.

    2009-09-16

    We describe a revolutionary new approach to high spectral resolution soft x-ray optics. Conventionally in the soft x-ray energy range, high spectral resolution is obtained by use of a relatively low line density grating operated in 1st order with small slits. This severely limits throughput. This limitation can be removed by use of a grating either in very high order, or with very high line density, if one can maintain high diffraction efficiency. We have developed a new technology for achieving both of these goals which should allow high throughput spectroscopy, at resolving powers of up to 106 at 1 keV. Such optics should provide a revolutionary advance for high resolution lifetime free spectroscopy, such as RIXS, and for pulse compression of chirped beams. We report recent developmental fabrication and characterization of a prototype grating optimized for 14.2 nm EUV light. The prototype grating with a 200 nm period of the blazed grating substrate coated with 20 Mo/Si bilayers with a period of 7.1 nm demonstrates good dispersion in the third order (effective groove density of 15,000 lines per mm) with a diffraction efficiency of more than 33percent.

  12. Research on Complicated Imaging Condition of GEO Optical High Resolution Earth Observing Satellite

    NASA Astrophysics Data System (ADS)

    Guo, Linghua

    2012-07-01

    The requirement for high time and space resolution of optical remote sensing satellite in disaster, land resources, environment, marine monitoring and meteorology observation, etc is getting urgent and strict. For that reason, a remote sensing satellite system solely located in MEO or LEO cannot operate continuous observation and Surveillance. GEO optical high resolution earth observing satellite in the other hand can keep the mesoscale and microscale target under continuous surveillance by controlling line of sight(LOS), and can provide imaging observation of an extensive region in a short time. The advantages of GEO satellite such as real-time observation of the mesoscale and microscale target, rapid response of key events, have been recognized by lots of countries and become a new trend of remote sensing satellite. As many advantages as the GEO remote sensing satellite has, its imaging condition is more complicated. Many new characteristics of imaging observation and imaging quality need to be discussed. We analyze each factor in the remote sensing link, using theoretical analysis and modeling simulation to get coefficient of each factor to represent its effect on imaging system. Such research achievements can provide reference for satellite mission analysis and system design.

  13. High-resolution imaging characterization of bladder dynamic morphophysiology by time-lapse optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Pan, Y. T.; Wu, Q.; Wang, Z. G.; Brink, P. R.; Du, C. W.

    2005-09-01

    We report an experimental study of the possibility of high-speed optical coherence tomography (OCT) for high-resolution imaging characterization of detrusor dynamic morphophysiology and analysis of the mechanisms that lead to geriatric incontinence (GI). The spontaneous contractility of intact fresh rabbit bladders was imaged with two-dimensional (2D) OCT ex vivo at up to 8frames/s. The time-lapse 2D OCT images were postprocessed by image segmentation and fast-Fourier-transform analysis to characterize the dynamic morphological changes of the bladder contractility. In addition, we studied young and aging rat bladders to analyze the differences in dynamics. Preliminary results of our ex vivo study reveal that time-lapse OCT can track the contractile waves of bladders at high spatial resolution and characterize their dynamic morphophysiology in terms of amplitude, phase, and frequency. The results suggest that time-lapse OCT has the potential to act as a detrusor optical biopsy to enhance the diagnosis of detrusor dysfunction and thus of the mechanisms that lead to GI.

  14. Optically assisted high-speed, high resolution analog-to-digital conversion (Invited Paper)

    NASA Astrophysics Data System (ADS)

    Zmuda, Henry; Hanna, Shane; Bussjager, R. J.; Fanto, M. L.; Hayduk, M. J.; Johns, S. T.; Malowicki, J. E.; Repak, P. L.

    2005-05-01

    An approach that modifies an analog fiber optic link with a recirculating optical loop as a means to realize a high-speed, high-resolution Analog-to-Digital Converted (ADC) is presented. The loops stores a time-limited microwave signal so that it may be digitized by using a slower, conventional electronic ADC. Detailed analytical analysis of the dynamic range and noise figure shows that under appropriate conditions the microwave signal degradation is sufficiently small so as to allow the digitization of a multi-gigahertz signal with a resolution greater than 10 effective bits. Experimental data is presented which shows that a periodic extension of the input signal can be sustained for well over one hundred periods that in turn suggests an electronic ADC speed-up factor of over 100. The data also shows that polarization effects must be carefully managed to inhibit the loops tendency to lase even though the loop itself contains no frequency-selective elements.

  15. High-resolution retinal imaging using adaptive optics and Fourier-domain optical coherence tomography

    DOEpatents

    Olivier, Scot S.; Werner, John S.; Zawadzki, Robert J.; Laut, Sophie P.; Jones, Steven M.

    2010-09-07

    This invention permits retinal images to be acquired at high speed and with unprecedented resolution in three dimensions (4.times.4.times.6 .mu.m). The instrument achieves high lateral resolution by using adaptive optics to correct optical aberrations of the human eye in real time. High axial resolution and high speed are made possible by the use of Fourier-domain optical coherence tomography. Using this system, we have demonstrated the ability to image microscopic blood vessels and the cone photoreceptor mosaic.

  16. High-resolution full-field optical coherence tomography using high dynamic range image processing

    NASA Astrophysics Data System (ADS)

    Leong-Hoï, A.; Claveau, R.; Montgomery, P. C.; Serio, B.; Uhring, W.; Anstotz, F.; Flury, M.

    2016-04-01

    Full-field optical coherence tomography (FF-OCT) based on white-light interference microscopy, is an emerging noninvasive imaging technique for characterizing biological tissue or optical scattering media with micrometer resolution. Tomographic images can be obtained by analyzing a sequence of interferograms acquired with a camera. This is achieved by scanning an interferometric microscope objectives along the optical axis and performing appropriate signal processing for fringe envelope extraction, leading to three-dimensional imaging over depth. However, noise contained in the images can hide some important details or induce errors in the size of these details. To firstly reduce temporal and spatial noise from the camera, it is possible to apply basic image post processing methods such as image averaging, dark frame subtraction or flat field division. It has been demonstrate that this can improve the quality of microscopy images by enhancing the signal to noise ratio. In addition, the dynamic range of images can be enhanced to improve the contrast by combining images acquired with different exposure times or light intensity. This can be made possible by applying a hybrid high dynamic range (HDR) technique, which is proposed in this paper. High resolution tomographic analysis is thus performed using a combination of the above-mentioned image processing techniques. As a result, the lateral resolution of the system can be improved so as to approach the diffraction limit of the microscope as well as to increase the power of detection, thus enabling new sub-diffraction sized structures contained in a transparent layer, initially hidden by the noise, to be detected.

  17. High-resolution lidar observations of mesospheric sodium and implications for adaptive optics.

    PubMed

    Pfrommer, Thomas; Hickson, Paul

    2010-11-01

    Observations of sodium density variability in the upper mesosphere/lower thermosphere, obtained using a high-resolution lidar system, show rapid fluctuations in the sodium centroid altitude. The temporal power spectrum extends above 1 Hz and is well-fit by a power law having a slope that is -1.95±0.12. These fluctuations produce focus errors in adaptive optics systems employing continuous-wave sodium laser guide stars, which can be significant for large-aperture telescopes. For a 30 m aperture diameter, the associated rms wavefront error is approximately 4 nm per meter of altitude change and increases as the square of the aperture diameter. The vertical velocity of the sodium centroid altitude is found to be ~23 ms(-1) on a 1 s time scale. If these high-frequency fluctuations arise primarily from advection of horizontal structure by the mesospheric wind, our data imply that variations in the sodium centroid altitude on the order of tens of meters occur over the horizontal scales spanned by proposed laser guide star asterisms. This leads to substantial differential focus errors (~107 nm over a 1 arc min separation with a 30 m aperture diameter) that may impact the performance of wide-field adaptive optics systems. Short-lasting and narrow sodium density enhancements, more than 1 order of magnitude above the local sodium density, occur due to advection of meteor trails. These have the ability to change the sodium centroid altitude by as much as 1 km in less than 1 s, which could result in temporary disruption of adaptive optics systems. PMID:21045896

  18. High-resolution flying-PIV with optical fiber laser delivery

    NASA Astrophysics Data System (ADS)

    Weichselbaum, Noah A.; André, Matthieu A.; Rahimi-Abkenar, Morteza; Manzari, Majid T.; Bardet, Philippe M.

    2016-05-01

    Implementation of non-intrusive optical measurement techniques, such as particle image velocimetry (PIV), in harsh environments requires specialized techniques for introducing controlled laser sheets to the region of interest. Large earthquake shake tables are a particularly challenging environment. Lasers must be mounted away from the table, and the laser sheet has to be delivered precisely and stably to the measurement station. Here, high-power multi-mode step-index fiber optics enable introduction of light from an Nd:YLF pulsed laser to a remote test section. Such lasers are suitable for coupling to optical fibers, which presents a portable, flexible, and safe manner to deliver a PIV light sheet. Best practices for their implementation are reviewed. Particular attention is focused on obtaining a collimated beam of acceptable quality at the output of the fiber. To achieve high spatial resolution, the PIV camera is directly mounted on the moving shake table with care to minimize its vibrations. A special arrangement of PIV planes is deployed for precise in-situ PIV alignment and to monitor and account for residual structure vibrations and beam wandering. The design of the instruments is detailed. Here, an experimental facility for the study of nuclear fuel bundle response to seismic forcing near prototypical conditions is instrumented. Only through integration of a high-resolution flying-PIV system can velocity fields be acquired. Data indicate that in the presence of a mean axial flow, a secondary oscillatory flow develops as the bundle oscillates. Instantaneous, phase-averaged, and fluctuating velocity fields illustrate this phenomenon.

  19. Optical design of a dynamic focus catheter for high-resolution endoscopic optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Meemon, Panomsak; Lee, Kye-Sung; Murali, Supraja; Rolland, Jannick

    2008-05-01

    The optical system design of a dynamic focus endoscopic probe for optical coherence tomography is reported. The dynamic focus capability is based on a liquid lens technology that provides variable focus by changing its curvatures in response to an electric field variation. The effects of a cylindrical exit window present, in practice, for a catheter were accounted for. Degradation in image quality caused by this window was corrected to get diffraction limited imaging performance. As a result, the dynamically focusing catheter with a lateral resolution ranging from 4 to 6 μm through an ~5 mm imaging distance was designed without mechanically refocusing the system.

  20. High-resolution adaptive optics scanning laser ophthalmoscope with dual deformable mirrors for large aberration correction

    SciTech Connect

    Chen, D; Jones, S M; Silva, D A; Olivier, S S

    2007-01-25

    Scanning laser ophthalmoscopes with adaptive optics (AOSLO) have been shown previously to provide a noninvasive, cellular-scale view of the living human retina. However, the clinical utility of these systems has been limited by the available deformable mirror technology. In this paper, we demonstrate that the use of dual deformable mirrors can effectively compensate large aberrations in the human retina, making the AOSLO system a viable, non-invasive, high-resolution imaging tool for clinical diagnostics. We used a bimorph deformable mirror to correct low-order aberrations with relatively large amplitudes. The bimorph mirror is manufactured by Aoptix, Inc. with 37 elements and 18 {micro}m stroke in a 10 mm aperture. We used a MEMS deformable mirror to correct high-order aberrations with lower amplitudes. The MEMS mirror is manufactured by Boston Micromachine, Inc with 144 elements and 1.5 {micro}m stroke in a 3 mm aperture. We have achieved near diffraction-limited retina images using the dual deformable mirrors to correct large aberrations up to {+-} 3D of defocus and {+-} 3D of cylindrical aberrations with test subjects. This increases the range of spectacle corrections by the AO systems by a factor of 10, which is crucial for use in the clinical environment. This ability for large phase compensation can eliminate accurate refractive error fitting for the patients, which greatly improves the system ease of use and efficiency in the clinical environment.

  1. Probing Protein Folding Kinetics with High-resolution, Stabilized Optical Tweezers

    NASA Astrophysics Data System (ADS)

    Wong, Wesley; Halvorsen, Ken

    2009-03-01

    Single-molecule techniques provide a powerful means of exploring molecular transitions such as the unfolding and refolding of a protein. However, the quantification of bi-directional transitions and near-equilibrium phenomena poses unique challenges, and is often limited by the detection resolution and long-term stability of the instrument. We have developed unique optical tweezers methods that address these problems, including an interference-based method for high-resolution 3D bead tracking (˜1 nm laterally, ˜0.3 nm vertically, at > 100 Hz), and a continuous autofocus system that stabilizes the trap height to within 1-2 nm longterm [1,2]. We have used our instruments to quantify the force-dependent unfolding and refolding kinetics of single protein domains (e.g. spectrin in collaboration with E. Evans). These single-molecule studies are presented, together with the accompanying probabilistic analysis that we have developed. References: 1. W.P. Wong, V. Heinrich, E. Evans, Mat. Res. Soc. Symp. Proc., 790, P5.1-P5.10 (2004). 2. V. Heinrich, W.P. Wong, K. Halvorsen, E. Evans, Langmuir, 24, 1194-1203 (2008).

  2. Ultra-high resolution water window x ray microscope optics design and analysis

    NASA Technical Reports Server (NTRS)

    Shealy, David L.; Wang, C.

    1993-01-01

    This project has been focused on the design and analysis of an ultra-high resolution water window soft-x-ray microscope. These activities have been accomplished by completing two tasks contained in the statement of work of this contract. The new results from this work confirm: (1) that in order to achieve resolutions greater than three times the wavelength of the incident radiation, it will be necessary to use spherical mirror surfaces and to use graded multilayer coatings on the secondary in order to accommodate the large variations of the angle of incidence over the secondary when operating the microscope at numerical apertures of 0.35 or greater; (2) that surface contour errors will have a significant effect on the optical performance of the microscope and must be controlled to a peak-to-valley variation of 50-100 A and a frequency of 8 periods over the surface of a mirror; and (3) that tolerance analysis of the spherical Schwarzschild microscope has been shown that the water window operations will require 2-3 times tighter tolerances to achieve a similar performance of operations with 130 A radiation. These results have been included in a manuscript included in the appendix.

  3. High Resolution Imaging with Adaptive Optics at the Multiple Mirror Telescope

    NASA Astrophysics Data System (ADS)

    Lloyd-Hart, M.; McLeod, B. A.; Wittman, D.; Colucci, D.; McCarthy, D. W.; Angel, R.; Dekany, R.

    1992-12-01

    We present the latest results from an adaptive optics program being implemented at the MMT using a six element adaptive mirror. The tilt of the wavefront over each of the six telescopes is determined with a Shack-Hartmann type sensor using a 24times 24 pixel low-noise CCD. This system allows the MMT to operate at a resolution of 0.3'' at 2 microns -- near the diffraction limit of the individual 1.8-m telescopes. This resolution can be obtained within ~ 1' of any star with visual magnitude < 16, allowing high-resolution near-IR imaging with a NICMOS2 array of a wide variety of targets, including high-redshift galaxies and young and evolved stars. This system can also be used with the MMT operated as a phased array telescope. In this mode, the piston errors between the telescopes are determined by examining the Fourier transform of the combined 2-micron image of the natural guide star using a fast-readout InSb array. In this configuration we have achieved a resolution of 0.075''. In good seeing we expect to obtain images of interest within the isoplanatic patch of guide stars with K magnitude <7. We acknowledge financial support from the NSF (AST92-03336) and the Flintridge Foundation. The adaptive mirror was donated by ThermoTrex Corp.

  4. High-resolution optical coherence tomographic imaging of osteoarthritic cartilage during open knee surgery

    PubMed Central

    Li, Xingde; Martin, Scott; Pitris, Costas; Ghanta, Ravi; Stamper, Debra L; Harman, Michelle; Fujimoto, James G; Brezinski, Mark E

    2005-01-01

    This study demonstrates the first real-time imaging in vivo of human cartilage in normal and osteoarthritic knee joints at a resolution of micrometers, using optical coherence tomography (OCT). This recently developed high-resolution imaging technology is analogous to B-mode ultrasound except that it uses infrared light rather than sound. Real-time imaging with 11-μm resolution at four frames per second was performed on six patients using a portable OCT system with a handheld imaging probe during open knee surgery. Tissue registration was achieved by marking sites before imaging, and then histologic processing was performed. Structural changes including cartilage thinning, fissures, and fibrillations were observed at a resolution substantially higher than is achieved with any current clinical imaging technology. The structural features detected with OCT were evident in the corresponding histology. In addition to changes in architectural morphology, changes in the birefringent or the polarization properties of the articular cartilage were observed with OCT, suggesting collagen disorganization, an early indicator of osteoarthritis. Furthermore, this study supports the hypothesis that polarization-sensitive OCT may allow osteoarthritis to be diagnosed before cartilage thinning. This study illustrates that OCT, which can eventually be developed for use in offices or through an arthroscope, has considerable potential for assessing early osteoarthritic cartilage and monitoring therapeutic effects for cartilage repair with resolution in real time on a scale of micrometers. PMID:15743479

  5. A system for optical high resolution screening of electrical excitable cells.

    PubMed

    Müller, Oliver; Tian, Qinghai; Zantl, Roman; Kahl, Valentin; Lipp, Peter; Kaestner, Lars

    2010-03-01

    The application of primary excitable cells for high content screening (HCS) requires a multitude of novel developments including cell culture and multi-well plates. Here we introduce a novel system combining optimised culture conditions of primary adult cardiomyocytes with the particular needs of excitable cells for arbitrary field stimulation of individual wells. The major advancements of our design were tested in calcium imaging experiments and comprise (i) each well of the plate can be subjected to individual pulse protocols, (ii) the software driving electrical stimulation can run as a stand-alone application but also as a plug-in in HCS software packages, (iii) the optical properties of the plastic substrate (foil) resemble those of glass coverslips fostering high resolution immersion-based microscopy, (iv) the bottom of the foil is coated with an oleophobic layer that prevents immersion oil from sticking, (v) the top of the foil is coated with an elastic film. The latter enables cardiomyocytes to display loaded contractions by mimicking the physiologically occurring local elastic network (e.g. extracellular matrix) and results in significantly increased contractions (with identical calcium transients) when compared to non-elastic substrates. Thus, our novel design and culture conditions represent an essential further step towards the application of primary cultured adult cardiomyocytes for HCS applications. PMID:20036001

  6. Measurement of ciliary beat frequency using ultra-high resolution optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Chen, Jason J.; Jing, Joseph C.; Su, Erica; Badger, Christopher; Coughlan, Carolyn A.; Chen, Zhongping; Wong, Brian J. F.

    2016-02-01

    Ciliated epithelial cells populate up to 80% of the surface area of the human airway and are responsible for mucociliary transport, which is the key protective mechanism that provides the first line of defense in the respiratory tract. Cilia beat in a rhythmic pattern and may be easily affected by allergens, pollutants, and pathogens, altering ciliary beat frequency (CBF) subsequently. Diseases including cystic fibrosis, chronic obstructive pulmonary disease, and primary ciliary dyskinesia may also decrease CBF. CBF is therefore a critical component of respiratory health. The current clinical method of measuring CBF is phase-contrast microscopy, which involves a tissue biopsy obtained via brushing of the nasal cavity. While this method is minimally invasive, the tissue sample must be oriented to display its profile view, making the visualization of a single layer of cilia challenging. In addition, the conventional method requires subjective analysis of CBF, e.g., manually counting by visual inspection. On the contrary, optical coherence tomography (OCT) has been used to study the retina in ophthalmology as well as vasculature in cardiology, and offers higher resolution than conventional computed tomography and magnetic resonance imaging. Based on this technology, our lab specifically developed an ultra-high resolution OCT system to image the microstructure of the ciliated epithelial cells. Doppler analysis was also performed to determine CBF. Lastly, we also developed a program that utilizes fast Fourier transform to determine CBF under phase-contrast microscopy, providing a more objective method compared to the current method.

  7. Ultra-high resolution, polarization sensitive transversal optical coherence tomography for structural analysis and strain mapping

    NASA Astrophysics Data System (ADS)

    Wiesauer, Karin; Pircher, Michael; Goetzinger, Erich; Hitzenberger, Christoph K.; Engelke, Rainer; Ahrens, Gisela; Pfeiffer, Karl; Ostrzinski, Ute; Gruetzner, Gabi; Oster, Reinhold; Stifter, David

    2006-02-01

    Optical coherence tomography (OCT) is a contactless and non-invasive technique nearly exclusively applied for bio-medical imaging of tissues. Besides the internal structure, additionally strains within the sample can be mapped when OCT is performed in a polarization sensitive (PS) way. In this work, we demonstrate the benefits of PS-OCT imaging for non-biological applications. We have developed the OCT technique beyond the state-of-the-art: based on transversal ultra-high resolution (UHR-)OCT, where an axial resolution below 2 μm within materials is obtained using a femtosecond laser as light source, we have modified the setup for polarization sensitive measurements (transversal UHR-PS-OCT). We perform structural analysis and strain mapping for different types of samples: for a highly strained elastomer specimen we demonstrate the necessity of UHR-imaging. Furthermore, we investigate epoxy waveguide structures, photoresist moulds for the fabrication of micro-electromechanical parts (MEMS), and the glass-fibre composite outer shell of helicopter rotor blades where cracks are present. For these examples, transversal scanning UHR-PS-OCT is shown to provide important information about the structural properties and the strain distribution within the samples.

  8. Arrhythmogenic Substrate of the Pulmonary Veins Assessed by High-Resolution Optical Mapping

    PubMed Central

    Arora, Rishi; Verheule, Sander; Scott, Luis; Navarrete, Antonio; Katari, Vikram; Wilson, Emily; Vaz, Dev; Olgin, Jeffrey E.

    2007-01-01

    Background It has recently been recognized that atrial fibrillation can originate from focal sources in the pulmonary veins (PVs). However, the mechanisms of focal atrial fibrillation have not been well characterized. We assessed the electrophysiological characteristics of the PVs using high-resolution optical mapping. Methods and Results Coronary-perfused, isolated whole-atrial preparations from 33 normal dogs were studied. Programmed electrical stimulation was performed, and a 4-cm2 area of the PV underwent optical mapping of transmembrane voltage to obtain 256 simultaneous action potentials. Marked conduction slowing was seen at the proximal PV, compared with the rest of the vein, on both the epicardial (31.3±4.47 versus 90.2±20.7 cm/s, P=0.001) and endocardial (45.8±6.90 versus 67.6±10.4 cm/s, P=0.012) aspects. Pronounced repolarization heterogeneity was also noted, with action potential duration at 80% repolarization being longest at the PV endocardium. Nonsustained reentrant beats were induced with single extrastimuli, and the complete reentrant loop was visualized (cycle length, 155±30.3 ms); reentrant activity could be sustained with isoproterenol. Sustained focal discharge (cycle length, 330 to 1100 ms) was seen from the endocardial surface in the presence of isoproterenol; each focus was localized near the venous ostium. Conclusions The normal PV seems to have the necessary substrate to support reentry as well as focal activity. Although reentry occurred more distally in the vein, focal activity seemed to occur more proximally. PMID:12665495

  9. Electrodynamics and energy characteristics of aurora at high resolution by optical methods

    NASA Astrophysics Data System (ADS)

    Dahlgren, H.; Lanchester, B. S.; Ivchenko, N.; Whiter, D. K.

    2016-06-01

    Technological advances leading to improved sensitivity of optical detectors have revealed that aurora contains a richness of dynamic and thin filamentary structures, but the source of the structured emissions is not fully understood. In addition, high-resolution radar data have indicated that thin auroral arcs can be correlated with highly varying and large electric fields, but the detailed picture of the electrodynamics of auroral filaments is yet incomplete. The Auroral Structure and Kinetics (ASK) instrument is a state-of-the-art ground-based instrument designed to investigate these smallest auroral features at very high spatial and temporal resolution, by using three electron multiplying CCDs in parallel for three different narrow spectral regions. ASK is specifically designed to utilize a new optical technique to determine the ionospheric electric fields. By imaging the long-lived O+ line at 732 nm, the plasma flow in the region can be traced, and since the plasma motion is controlled by the electric field, the field strength and direction can be estimated at unprecedented resolution. The method is a powerful tool to investigate the detailed electrodynamics and current systems around the thin auroral filaments. The two other ASK cameras provide information on the precipitation by imaging prompt emissions, and the emission brightness ratio of the two emissions, together with ion chemistry modeling, is used to give information on the energy and energy flux of the precipitating electrons. In this paper, we discuss these measuring techniques and give a few examples of how they are used to reveal the nature and source of fine-scale structuring in the aurora.

  10. Insulated Conducting Cantilevered Nanotips and Two-Chamber Recording System for High Resolution Ion Sensing AFM

    PubMed Central

    Meckes, Brian; Arce, Fernando Teran; Connelly, Laura S.; Lal, Ratnesh

    2014-01-01

    Biological membranes contain ion channels, which are nanoscale pores allowing controlled ionic transport and mediating key biological functions underlying normal/abnormal living. Synthetic membranes with defined pores are being developed to control various processes, including filtration of pollutants, charge transport for energy storage, and separation of fluids and molecules. Although ionic transport (currents) can be measured with single channel resolution, imaging their structure and ionic currents simultaneously is difficult. Atomic force microscopy enables high resolution imaging of nanoscale structures and can be modified to measure ionic currents simultaneously. Moreover, the ionic currents can also be used to image structures. A simple method for fabricating conducting AFM cantilevers to image pore structures at high resolution is reported. Tungsten microwires with nanoscale tips are insulated except at the apex. This allows simultaneous imaging via cantilever deflections in normal AFM force feedback mode as well as measuring localized ionic currents. These novel probes measure ionic currents as small as picoampere while providing nanoscale spatial resolution surface topography and is suitable for measuring ionic currents and conductance of biological ion channels. PMID:24663394

  11. Fast, high-resolution 3D dosimetry utilizing a novel optical-CT scanner incorporating tertiary telecentric collimation.

    PubMed

    Sakhalkar, H S; Oldham, M

    2008-01-01

    This study introduces a charge coupled device (CCD) area detector based optical-computed tomography (optical-CT) scanner for comprehensive verification of radiation dose distributions recorded in nonscattering radiochromic dosimeters. Defining characteristics include: (i) a very fast scanning time of approximately 5 min to acquire a complete three-dimensional (3D) dataset, (ii) improved image formation through the use of custom telecentric optics, which ensures accurate projection images and minimizes artifacts from scattered and stray-light sources, and (iii) high resolution (potentially 50 microm) isotropic 3D dose readout. The performance of the CCD scanner for 3D dose readout was evaluated by comparison with independent 3D readout from the single laser beam OCTOPUS-scanner for the same PRESAGE dosimeters. The OCTOPUS scanner was considered the "gold standard" technique in light of prior studies demonstrating its accuracy. Additional comparisons were made against calculated dose distributions from the ECLIPSE treatment-planning system. Dose readout for the following treatments were investigated: (i) a single rectangular beam irradiation to investigate small field and very steep dose gradient dosimetry away from edge effects, (ii) a 2-field open beam parallel-opposed irradiation to investigate dosimetry along steep dose gradients, and (iii) a 7-field intensity modulated radiation therapy (IMRT) irradiation to investigate dosimetry for complex treatment delivery involving modulation of fluence and for dosimetry along moderate dose gradients. Dose profiles, dose-difference plots, and gamma maps were employed to evaluate quantitative estimates of agreement between independently measured and calculated dose distributions. Results indicated that dose readout from the CCD scanner was in agreement with independent gold-standard readout from the OCTOPUS-scanner as well as the calculated ECLIPSE dose distribution for all treatments, except in regions within a few

  12. Development of a High-performance Optical System and Fluorescent Converters for High-resolution Neutron Imaging

    NASA Astrophysics Data System (ADS)

    Sakai, T.; Yasuda, R.; Iikura, H.; Nojima, T.; Matsubayashi, M.

    Two novel devices for use in neutron imaging technique are introduced. The first one is a high-performance optical lens for video camera systems. The lens system has a magnification of 1:1 and an F value of 3. The optical resolution is less than 5 μm. The second device is a high-resolution fluorescent plate that converts neutrons into visible light. The fluorescent converter material consists of a mixture of 6LiF and ZnS(Ag) fine powder, and the thickness of the converter is material is as little as 15 μm. The surface of the plate is coated with a 1 μm-thick gadolinium oxide layer. This layer is optically transparent and acts as an electron emitter for neutron detection. Our preliminary results show that the developed optical lens and fluorescent converter plates are very promising for high-resolution neutron imaging.

  13. Very High Resolution Solar X-ray Imaging Using Diffractive Optics

    NASA Technical Reports Server (NTRS)

    Dennis, B. R.; Skinner, G. K.; Li, M. J.; Shih, A. Y.

    2012-01-01

    This paper describes the development of X-ray diffractive optics for imaging solar flares with better than 0.1 arcsec angular resolution. X-ray images with this resolution of the greater than or equal to 10 MK plasma in solar active regions and solar flares would allow the cross-sectional area of magnetic loops to be resolved and the coronal flare energy release region itself to be probed. The objective of this work is to obtain X-ray images in the iron-line complex at 6.7 keV observed during solar flares with an angular resolution as fine as 0.1 arcsec - over an order of magnitude finer than is now possible. This line emission is from highly ionized iron atoms, primarily Fe xxv, in the hottest flare plasma at temperatures in excess of approximately equal to 10 MK. It provides information on the flare morphology, the iron abundance, and the distribution of the hot plasma. Studying how this plasma is heated to such high temperatures in such short times during solar flares is of critical importance in understanding these powerful transient events, one of the major objectives of solar physics.We describe the design, fabrication, and testing of phase zone plate X-ray lenses with focal lengths of approximately equal to 100 m at these energies that would be capable of achieving these objectives. We show how such lenses could be included on a two-spacecraft formation-flying mission with the lenses on the spacecraft closest to the Sun and an X-ray imaging array on the second spacecraft in the focal plane approximately equal to 100 m away. High resolution X-ray images could be obtained when the two spacecraft are aligned with the region of interest on the Sun. Requirements and constraints for the control of the two spacecraft are discussed together with the overall feasibility of such a formation-flying mission.

  14. Deriving High Resolution UV Aerosol Optical Depth over East Asia using CAI-OMI Joint Retrieval

    NASA Astrophysics Data System (ADS)

    Go, S.; Kim, J.; KIM, M.; Lee, S.

    2015-12-01

    Monitoring aerosols using near UV spectral region have been successfully performed over decades by Ozong Monitoring Instruments (OMI) with benefit of strong aerosol signal over continuous dark surface reflectance, both land and ocean. However, because of big foot print of OMI, the cloud contamination error was a big issue in the UV aerosol algorithm. In the present study, high resolution UV aerosol optical depth (AOD) over East Asia was derived by collaborating the Greenhouse gases Observing SATellite/Thermal And Near infrared Sensor for carbon Observation (GOSAT/TANSO)-Cloud and Aerosol Imager (CAI) and OMI together. AOD of 0.1 degree grid resolution was retrieved using CAI band 1 (380nm) by bring OMI lv.2 aerosol type, single scattering albedo, and aerosol layer peak height in 1 degree grid resolution. Collocation of the two dataset within the 0.5 degree grid with time difference of OMI and CAI less than 5 minute was selected. Selected region becomes wider as it goes to the higher latitude. Also, calculated degradation factor of 1.57 was applied to CAI band1 (380nm) by comparing normalized radiance and Lambertian Equivalent Reflectivity (LER) of both sensors. The calculated degradation factor was reasonable over dark scene, but inconsistent over cirrus cloud and bright area. Then, surface reflectance was developed by compositing CAI LER minimum data over three month period, since the infrequent sampling rate associated with the three-day recursion period of GOSAT and the narrow CAI swath of 1000 km. To retrieve AOD, look up table (LUT) was generated using radiative transfer model VLIDORT NGST. Finally, the retrieved AOD was validated with AERONET ground based measurement data during the Dragon-NE Asia campaign in 2012.

  15. Vertical and horizontal corneal epithelial thickness profiles determined by ultra-high resolution optical coherence tomography

    PubMed Central

    Du, Chixin; Wang, Jianhua; Cui, Lele; Shen, Meixiao; Yuan, Yimin

    2011-01-01

    Purpose To measure vertical and horizontal thickness profiles of the central and peripheral corneal epithelium and determine if daytime changes occur. Methods Forty eyes of 20 normal subjects were imaged by ultra-high resolution spectral domain optical coherence tomography to profile the corneal epithelial thickness from the edge of Bowman’s layer to the central cornea across the vertical and horizontal meridians. Measurements were made at 10:00 AM and again at 6, 8 hours later. Results The baseline vertical meridional epithelial thickness was thinnest, 42.9±4.1 μm, at the edge of Bowman’s layer in the superior region. It increased in thickness (p<0.01), towards the central cornea. The central epithelium averaged 52.5±2.4 μm, becoming thickest, 55.2±2.5 μm, in the inferior pericentral region. It thinned towards the inferior periphery, reaching 51.3±5.1 μm at the edge of Bowman’s layer (p<0.01). Along the horizontal meridian, the epithelium was thickest at the nasal side, 58.6±5.1 μm, and temporal side, 59.3±6.6 μm, near the edges of Bowman’s layer. It thinned towards the central cornea. There were no significant changes in the epithelial thickness at any location over 8 hours. Conclusion Epithelial thickness varied over the horizontal and vertical meridians and appeared stable during the daytime. PMID:22357393

  16. Investigation of solar active regions at high resolution by balloon flights of the solar optical universal polarimeter, extended definition phase

    NASA Technical Reports Server (NTRS)

    Tarbell, Theodore D.

    1993-01-01

    Technical studies of the feasibility of balloon flights of the former Spacelab instrument, the Solar Optical Universal Polarimeter, with a modern charge-coupled device (CCD) camera, to study the structure and evolution of solar active regions at high resolution, are reviewed. In particular, different CCD cameras were used at ground-based solar observatories with the SOUP filter, to evaluate their performance and collect high resolution images. High resolution movies of the photosphere and chromosphere were successfully obtained using four different CCD cameras. Some of this data was collected in coordinated observations with the Yohkoh satellite during May-July, 1992, and they are being analyzed scientifically along with simultaneous X-ray observations.

  17. A high-resolution hydroclimate record of the last three millennia from a cored stalagmite at desoto caverns (Alabama, USA)

    NASA Astrophysics Data System (ADS)

    Dhungana, Rajesh

    Late Holocene climate changes in the Southeast USA are poorly documented due to the paucity of high-resolution paleo-records. This study provides high-resolution records of rapid hydroclimate changes in the Southeast over the last three millennia. The records are based on stable isotope rainfall proxies whose time series are constrained by precise U/Th dates from a stalagmite sampled at DeSoto Caverns. The average growth rate of the stalagmite was 149 mum/yr prior to 1400 years and it has been growing with an average growth rate of 42 mum/yr in the last 1400 years. During the past three thousand years stable isotope time series document six wet episodes (at ˜ 2950, 2450, 1675, 1200, 700 and 70 years ago) alternating with six drier periods (at ˜ 3100, 2800, 1900, 1500, 800 and 300 years ago). The biannually resolved delta 18O record agrees well with the contemporaneous SST record from the Sargasso Sea cores suggesting that changes in moisture availability in the Southeast are likely linked to subtropical North Atlantic SST variability. Power spectra analysis of the stalagmite-based oxygen isotope record reveals statistically significant periodicities at 24+/-1 and 36+/-1 year that are consistent with those observed in the contemporaneous atmospheric 14C production record. The 24 years periodicity is also consistent with the 24-year NAO Index periodicity. On the basis of our analysis we propose that the hydroclimate in the Southeast USA over the last three millennia was intimately linked to NAO variability powered by solar activity fluctuations.

  18. Calibration of sclerosponge oxygen isotope records to temperature using high-resolution δ 18O data

    NASA Astrophysics Data System (ADS)

    Rosenheim, Brad E.; Swart, Peter K.; Willenz, Philippe

    2009-09-01

    A revised calibration is presented relating the oxygen isotope composition of the aragonite-secreting sclerosponge Ceratoporella nicholsoni, oxygen isotope composition of seawater, and ambient water temperature. This new relationship has been obtained using high-resolution δ 18O data measured in sclerosponges from the Bahamas and Jamaica compared to ambient temperature measurements and δ 18O values of seawater from the two locations, both measured and published. New data improve an existing calibration which was determined using measurements of salinity rather than directly measured δ 18O values of the seawater and was composed of measurements from different species of sclerosponge and other aragonite-secreting organisms. The updated calibration ( n = 12, r2 = 0.95) is: T(°C)=16.1(±3.1)-[6.5(±1.1)](δ-δ), where T is temperature in degrees Celsius, δ arag is the δ 18O value of aragonite normalized to VPDB, and δ sw is the δ 18O value of water normalized to VSMOW. This calibration improves accuracy and precision of Caribbean sclerosponges for reconstructions of temperature as well as δ 18O values of seawater.

  19. Non-harmonic analysis for high-resolution optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Chong, Changho; Cao, Xu; Morosawa, Atsushi; Totsuka, Koki; Suzuki, Takuya; Hirobayashi, Shigeki

    2010-02-01

    A novel processing technique called Non-Harmonic Analysis (NHA) is proposed for high-resolution OCT imaging. Conventional Fourier-Domain OCT relies on the FFT calculation which depends on the window function and length. NHA can resolve high frequency without being influenced by window function or frame length of sampled data. The results show that NHA process realizes practical image resolution equivalent to 100nm swept range by using significantly reduced wavelength range, and also implies the potential of high resolution imaging capability without the need of a broadband source.

  20. High-resolution Holocene South American monsoon history recorded by a speleothem from Botuverá Cave, Brazil

    NASA Astrophysics Data System (ADS)

    Bernal, J. P.; Cruz, Francisco W.; Stríkis, Nicolás M.; Wang, Xianfeng; Deininger, Michael; Catunda, Maria Carolina A.; Ortega-Obregón, C.; Cheng, Hai; Edwards, R. Lawrence; Auler, Augusto S.

    2016-09-01

    A Holocene stalagmite from Botuverá Cave, southeastern Brazil was analyzed by LA-ICPMS for Mg/Ca, Sr/Ca, Ba/Ca. The observed variability in the record was demonstrated to be modulated by prior calcite precipitation, and, thus, is interpreted to reflect monsoon intensity. We find that the calcite δ18O is strongly correlated with Sr/Ca, indicating that atmospheric circulation over South America and monsoon intensity have been tightly correlated throughout most of the Holocene, both directly responding to solar precession. Comparison with other contemporaneous high-resolution hydroclimate records reveals that SAMS has shown a degree of complexity during the Holocene not previously detected, with periods where the South American Convergence Zone (SACZ) expanded to cover most of the South American sub-continent, and coincident with periods of low-SST in the north Atlantic. We also detect periods where rainfall amount in northeastern and southeastern Brazil are markedly anti-phased, suggesting a north-south migration of SACZ, which it appears to be mediated by solar irradiance. The high-resolution nature of our record allow us to examine the effect that Holocene climate anomalies had upon SAMS dynamics and hydroclimate in southeastern Brazil, in particular the 8.2 ka event and the Little Ice Age. In addition to confirm the internal structure of the events, we also detect the possible consequences of the climatic anomalies upon ocean-atmosphere interactions through its effects upon SAMS.

  1. High resolution late Pliocene sea-surface temperature record from the northeast Atlantic Ocean

    USGS Publications Warehouse

    Dowsett, H.J.; Loubere, P.

    1992-01-01

    Application of quantitative micropaleontologic methods of sea-surface temperature (SST) estimation to the planktic foraminiferal faunas at Hole 548 in the northeast Atlantic between 3.5 and 2.0 Ma provide a detailed record of Pliocene surface temperature changes during the onset of major Northern Hemisphere glaciation. SST estimates indicate a relatively warm interval of "enhanced interglacials (warm peaks)" between 3.2 and 2.8 Ma followed by steady cooling with a significant step-like change toward colder temperatures occurring about 2.4 Ma. This record compares well with faunal and SST records from other North Atlantic sites. ?? 1992.

  2. Twentieth century sea-ice trends in the Ross Sea from a high-resolution, coastal ice-core record

    NASA Astrophysics Data System (ADS)

    Sinclair, Kate E.; Bertler, Nancy A. N.; Bowen, Melissa M.; Arrigo, Kevin R.

    2014-05-01

    We present the first proxy record of sea-ice area (SIA) in the Ross Sea, Antarctica, from a 130 year coastal ice-core record. High-resolution deuterium excess data show prevailing stable SIA from the 1880s until the 1950s, a 2-5% reduction from the mid-1950s to the early-1990s, and a 5% increase after 1993. Additional support for this reconstruction is derived from ice-core methanesulphonic acid concentrations and whaling records. While SIA has continued to decline around much of the West Antarctic coastline since the 1950s, concurrent with increasing air and ocean temperatures, the underlying trend is masked in the Ross Sea by a switch to positive SIA anomalies since the early-1990s. This increase is associated with a strengthening of southerly winds and the enhanced northward advection of sea ice.

  3. High-resolution intracellular recordings using a real-time computational model of the electrode.

    PubMed

    Brette, Romain; Piwkowska, Zuzanna; Monier, Cyril; Rudolph-Lilith, Michelle; Fournier, Julien; Levy, Manuel; Frégnac, Yves; Bal, Thierry; Destexhe, Alain

    2008-08-14

    Intracellular recordings of neuronal membrane potential are a central tool in neurophysiology. In many situations, especially in vivo, the traditional limitation of such recordings is the high electrode resistance and capacitance, which may cause significant measurement errors during current injection. We introduce a computer-aided technique, Active Electrode Compensation (AEC), based on a digital model of the electrode interfaced in real time with the electrophysiological setup. The characteristics of this model are first estimated using white noise current injection. The electrode and membrane contribution are digitally separated, and the recording is then made by online subtraction of the electrode contribution. Tests performed in vitro and in vivo demonstrate that AEC enables high-frequency recordings in demanding conditions, such as injection of conductance noise in dynamic-clamp mode, not feasible with a single high-resistance electrode until now. AEC should be particularly useful to characterize fast neuronal phenomena intracellularly in vivo. PMID:18701064

  4. Climate Change during Marine Isotope Stages 10 & 11 based on High-Resolution Speleothem Records from Eastern North America

    NASA Astrophysics Data System (ADS)

    Buckles, J. A.; Gao, Y.; Wang, X.; Rowe, H.; Cheng, H.; Edwards, R. L.

    2014-12-01

    Two speleothems from eastern North America grew throughout Marine Isotope Stages 10 and 11. High-resolution stable isotope δ18O and δ13C, and Sr records are constrained by 16 230Th age dates. MIS 11 (374 - 424 kyr BP) is of particular interest due to the similarity of orbitally-controlled insolation conditions from this time period and the modern. While few high-resolution continental paleoclimate records exist for this time period, marine records reveal that during this prominent interglacial, sea surface temperatures were relatively stable, in contrast to those which occurred during the subsequent glacial period (MIS 10). Speleothems TNBS-8 and TNMOR2-01, from Tennessee's Blue Springs Cave and Morrell Cave, respectively, grew between 338 - 420 kyr BP and overlap for ~50 kyr. Growth rates were generally higher during MIS 11 than 10, with a higher degree of variability. Stable isotope δ18O and δ13C records (TNMOR2-01) show an overall trend towards higher values throughout the MIS 11-10 transition, with well-defined periods of depletion and enrichment. Sr concentration measured through µ-XRF reveals similar behavior as the δ13C record and serves as a proxy for moisture availability. Overlapping Sr results for both speleothems share general trends and reveal distinct intervals of increased precipitation occur throughout the record, with most coinciding with summer insolation maxima. The transitions of MIS 11.2 to 11.1 and MIS 11 to 10 are well-constrained by abrupt increases in δ18O and δ13C values along with Sr concentrations. An examination of continental proxy responses to similar orbital and climatic conditions as the modern allows for not only a greater understanding of how the climate changed during the MIS 10-11, but also allows for the examination of natural climate variability in light of the addition of anthropogenic climate forcing.

  5. High-resolution 900 year volcanic and climatic record from the Vostok area, East Antarctica

    NASA Astrophysics Data System (ADS)

    Osipov, E. Y.; Khodzher, T. V.; Golobokova, L. P.; Onischuk, N. A.; Lipenkov, V. Y.; Ekaykin, A. A.; Shibaev, Y. A.; Osipova, O. P.

    2014-05-01

    Ion chromatography measurements of 1730 snow and firn samples obtained from three short cores and one pit in the Vostok station area, East Antarctica, allowed for the production of the combined volcanic record of the last 900 years (AD 1093-2010). The resolution of the record is 2-3 samples per accumulation year. In total, 24 volcanic events have been identified, including seven well-known low-latitude eruptions (Pinatubo 1991, Agung 1963, Krakatoa 1883, Tambora 1815, Huanaputina 1600, Kuwae 1452, El Chichon 1259) found in most of the polar ice cores. In comparison with three other East Antarctic volcanic records (South Pole, Plateau Remote and Dome C), the Vostok record contains more events within the last 900 years. The differences between the records may be explained by local glaciological conditions, volcanic detection methodology, and, probably, differences in atmospheric circulation patterns. The strongest volcanic signal (both in sulfate concentration and flux) was attributed to the AD 1452 Kuwae eruption, similar to the Plateau Remote and Talos Dome records. The average snow accumulation rate calculated between volcanic stratigraphic horizons for the period AD 1260-2010 is 20.9 mm H2O. Positive (+13%) anomalies of snow accumulation were found for AD 1661-1815 and AD 1992-2010, and negative (-12%) for AD 1260-1601. We hypothesized that the changes in snow accumulation are associated with regional peculiarities in atmospheric transport.

  6. Update on the Gemini High-Resolution Optical SpecTrograph (GHOST)

    NASA Astrophysics Data System (ADS)

    Margheim, Steven J.; Ghost Instrument Team

    2015-01-01

    The Gemini High-Resolution Opitcal SpecTrograph (GHOST) is under development for the Gemini telescopes in collaboration with the Austrailian Astronomical Observatory (AAO), the NRC-Herzberg in Canada, and the Australian National University (ANU). The latest design and project plan will be presented and the scientific role of the instrument will be discussed.

  7. Observation and Analysis of High Resolution Optical Line Profiles in Comet Hyakutake (C/1996 B2)

    NASA Astrophysics Data System (ADS)

    Combi, M. R.; Cochran, A. L.

    1997-07-01

    Very high resolution (R=200,000) and high signal-to-noise echelle spectra were obtained of comet C/Hyakutake 1996 B2 using the 2DCoude spectrograph on the 2.7 m telescope at McDonald Observatory during late March and early April 1996. Doppler resolved profiles are presented for individual lines of most of the major optical neutral species: CN, C_2, O((1) D) at 6300{ Angstroms}, O((1) S) at 5577{ Angstroms} , NH_2, and H Balmer-alpha at 6563{ Angstroms}. These may be the first ever to be published for CN, C_2, and O((1) S). In all cases the instrument spectral function is smaller than the intrinsic line widths of the individual cometary lines, so the observations provide clear signatures of lines which are Doppler broadened by different combinations of the coma expansion, exothermic photochemical ejection speeds, and collisional thermalization. For modeling analysis of these data we have used a hybrid fluid/kinetic Monte Carlo approach which can realistically include all of the relevant physical/chemical processes important for shaping the spectral lines. Because of the very short lifetime of the NH_2 parent (NH_3), the NH_2 is collisionally thermalized and provides an excellent probe of the outflow of the expanding coma. Because O((1) D) atoms in the region sampled are produced mainly by the photodissociation of water and the resulting photon is a prompt emission, the line retains signatures of both the basic coma expansion velocity and the 1.6 km s(-1) ejection speed of the O({(1}) D) atoms. The O((1) S) profile is consistent with that for the O((1) D). The profiles of CN and C_2 are somewhat broadened (CN more so than C_2), compared with NH_2, and seem to require a combination of coma expansion and the exothermic ejection speed they receive upon their production. Although the H Balmer-alpha line is complicated by a chance coincidence of an H_2O(+) line and optical depth effects in the solar Lyman-beta which pumps the Balmer-alpha emission, the spread of the wings

  8. A High-Resolution Record of Meltwater Discharge and Deglacial Warming in the Gulf of Mexico

    NASA Astrophysics Data System (ADS)

    Gilbert, M. L.; Hastings, D. W.; Flower, B. P.; Quinn, T. M.

    2005-12-01

    Sea surface temperature (SST) and δ18Osw records from the Gulf of Mexico (GOM) during the last deglaciation will help distinguish different mechanisms for abrupt climate change. Orca Basin is an ideal location to study climate changes in the GOM and to record the timing of meltwater discharge events through the Mississippi River; the anoxic hypersaline bottom water prevents bioturbation and results in finely laminated sediments. We sampled core MD 02-2550 at 0.5 cm resolution which, given sedimentation rates of greater than 30 cm/ka, corresponds to ~20 yr resolution. We analyzed Mg/Ca ratios on Globigerinoides ruber (white) to generate a SST record using the calibration of Anand et al. (2003). The record presented here extends from ca. 13 to 10.4 ka BP. The average temperatures at the beginning of the record from 13 to 12.5 ka BP are 26.3°C, with a variability of 1.0°C (1 σ). A major SST decrease of 3.0°C is recorded in two steps at 12.5 ka and 12.2 that lasts ca. 500 years. Within this transition, a more rapid cooling of ~2.0°C in less than 100 years is observed starting at ca. 12.2 ka. A reciprocal warming is observed in three stages from 12.0 to 11.0 ka: first a warming of 1.3°C over 300 years, relatively constant temperatures for 400 years, and a final warming of 1.7°C over 300 years returning to the same average SST as before the cold period. These cooler temperatures persisted for ca. 1500 yrs and likely corresponds to the Younger Dryas stadial. The annual SST in the GOM today is the same as the average temperature preceding and following the cold period. These trends represent the most detailed Mg/Ca SST records of the B/A Younger Dryas oscillation observed in the GOM. Paired with the Mg/Ca-derived SST, δ18O data will be generated to produce a δ18Osw record. This will enable us to ascertain the timing and precise phasing of deglacial warming relative to Laurentide ice sheet meltwater input into the GOM.

  9. Verlorenvlei - The first continuous Holocene high-resolution lake sediment record from the Winter Rainfall Zone of South Africa

    NASA Astrophysics Data System (ADS)

    Haberzettl, T.; Kasper, T.; Lederer, M.; Wündsch, M.; Frenzel, P.; Zabel, M.; Kirsten, K. L.; Meadows, M. E.; Quick, L. J.; St-Onge, G.; Maeusbacher, R.

    2015-12-01

    Verlorenvlei is a coastal lake in the Winter Rainfall Zone of the Western Cape Province of South Africa. Up to now several attempts have been made to recover sediment cores from this lake. However, no continuous high-resolution record covering large parts of the Holocene has been available so far. Within the project RAIN (Regional Archives for Integrated iNvestigations) it was possible to recover a 14.2 m paired parallel core from the central part of Verlorenvlei. Investigations on recent surface sediment distributions (elemental composition and grain sizes) indicate that this sediment core is very well suited for paleoenvironmental reconstructions. Using a set of 23 radiocarbon ages, a chronology for the past 9,000 cal BP was established which suggests continuous sedimentation over this period. Preliminary lithological and geochemical investigations show that this record can be used for sea level reconstructions as the lake was periodically inundated by the ocean during the past 9,000 cal BP. This is recorded in distinctly elevated Ca and Sr contents as well as the occurrence of marine indicator species (snail and mussel shells) in parts of the sediment core. Thin, pale grey layers of fine sediment occurring at various sediment depths seem to reflect event related deposits. In terms of lithology, geochemical and magnetic composition, the upper 50 cm clearly differ from the rest of the record and indicate increased sediment supply from the catchment, which is likely linked to anthropogenic farming activities. In conclusion, the newly recovered sediment record from Verlorenvlei offers excellent potential for a detailed, high-resolution reconstruction of sea level changes, climate variations and anthropogenic impact during the past 9,000 cal BP in an area in which natural archives are very scarce or poorly dated.

  10. High-resolution retinal imaging through open-loop adaptive optics

    NASA Astrophysics Data System (ADS)

    Li, Chao; Xia, Mingliang; Li, Dayu; Mu, Quanquan; Xuan, Li

    2010-07-01

    Using the liquid crystal spatial light modulator (LC-SLM) as the wavefront corrector, an open-loop adaptive optics (AO) system for fundus imaging in vivo is constructed. Compared with the LC-SLM closed-loop AO system, the light energy efficiency is increased by a factor of 2, which is helpful for the safety of fundus illumination in vivo. In our experiment, the subjective accommodation method is used to precorrect the defocus aberration, and three subjects with different myopia 0, -3, and -5 D are tested. Although the residual wavefront error after correction cannot to detected, the fundus images adequately demonstrate that the imaging system reaches the resolution of a single photoreceptor cell through the open-loop correction. Without dilating and cyclopleging the eye, the continuous imaging for 8 s is recorded for one of the subjects.

  11. Characterization of rock thermal conductivity by high-resolution optical scanning

    USGS Publications Warehouse

    Popov, Y.A.; Pribnow, D.F.C.; Sass, J.H.; Williams, C.F.; Burkhardt, H.

    1999-01-01

    We compared thress laboratory methods for thermal conductivity measurements: divided-bar, line-source and optical scanning. These methods are widely used in geothermal and petrophysical studies, particularly as applied to research on cores from deep scientific boreholes. The relatively new optical scanning method has recently been perfected and applied to geophysical problems. A comparison among these methods for determining the thermal conductivity tensor for anisotropic rocks is based on a representative collection of 80 crystalline rock samples from the KTB continental deep borehole (Germany). Despite substantial thermal inhomogeneity of rock thermal conductivity (up to 40-50% variation) and high anisotropy (with ratios of principal values attaining 2 and more), the results of measurements agree very well among the different methods. The discrepancy for measurements along the foliation is negligible (<1%). The component of thermal conductivity normal to the foliation reveals somewhat larger differences (3-4%). Optical scanning allowed us to characterize the thermal inhomogeneity of rocks and to identify a three-dimensional anisotropy in thermal conductivity of some gneiss samples. The merits of optical scanning include minor random errors (1.6%), the ability to record the variation of thermal conductivity along the sample, the ability to sample deeply using a slow scanning rate, freedom from constraints for sample size and shape, and quality of mechanical treatment of the sample surface, a contactless mode of measurement, high speed of operation, and the ability to measure on a cylindrical sample surface. More traditional methods remain superior for characterizing bulk conductivity at elevated temperature.Three laboratory methods including divided-bar, line-source and optical scanning are widely applied in geothermal and petrophysical studies. In this study, these three methods were compared for determining the thermal conductivity tensor for anisotropic rocks

  12. High-resolution detection of Brownian motion for quantitative optical tweezers experiments.

    PubMed

    Grimm, Matthias; Franosch, Thomas; Jeney, Sylvia

    2012-08-01

    We have developed an in situ method to calibrate optical tweezers experiments and simultaneously measure the size of the trapped particle or the viscosity of the surrounding fluid. The positional fluctuations of the trapped particle are recorded with a high-bandwidth photodetector. We compute the mean-square displacement, as well as the velocity autocorrelation function of the sphere, and compare it to the theory of Brownian motion including hydrodynamic memory effects. A careful measurement and analysis of the time scales characterizing the dynamics of the harmonically bound sphere fluctuating in a viscous medium directly yields all relevant parameters. Finally, we test the method for different optical trap strengths, with different bead sizes and in different fluids, and we find excellent agreement with the values provided by the manufacturers. The proposed approach overcomes the most commonly encountered limitations in precision when analyzing the power spectrum of position fluctuations in the region around the corner frequency. These low frequencies are usually prone to errors due to drift, limitations in the detection, and trap linearity as well as short acquisition times resulting in poor statistics. Furthermore, the strategy can be generalized to Brownian motion in more complex environments, provided the adequate theories are available. PMID:23005790

  13. A high-resolution geochemical record of Late Quaternary paleohydrological conditions from Chihuahuan Desert, Mexico

    NASA Astrophysics Data System (ADS)

    Quiroz-Jiménez, J.; Roy, P.

    2013-05-01

    Paleolake Babicora is located at 29°N in the northern Mexico and sediments deposited in the basin provide useful information about the late Quaternary paleohydrological conditions of Chihuahuan Desert. The proxy-records of productivity, pluvial discharge into the basin, lake water salinity and aeolian activity in surroundings of the basin over the last 80 cal ka BP were reconstructed by analyzing the concentrations of total organic carbon, carbonates, C/N, Ti, Sr and Zr/Al in a 976 cm long sediment core. During 80-58 cal ka BP, the pluvial discharge was higher and the water column was characterized by lower salinity (wet conditions). Terrestrial vegetation had higher influence on the organic carbon deposited between 71 and 53 cal ka BP. We record paleohydrological instability with millennial-scale fluctuations in the last 40 cal ka BP. Arid conditions possibly reached its maximum at ca. 40 cal ka BP, characterized with a hiatus in sedimentation related to aeolian activity. Comparison between the paleohydrological record from Babicora and variability of winter precipitation from southwest USA suggest that the runoff into Babicora was controlled by summer season precipitation. The periods of more pluvial discharge were contemporary to the north Atlantic interstadials and vice versa.

  14. A High-Resolution Upper Pleistocene Palaeomagnetic Record from the Fronte Section at Taranto, Italy

    NASA Astrophysics Data System (ADS)

    Lurcock, P. C.; Florindo, F.; Negri, A.; Marabini, S.; Mastronuzzi, G.; Morigi, C.; Vai, G. B.

    2015-12-01

    As part of a project to establish a global boundary stratotype section and point (GSSP) for the Upper Pleistocene, we have investigated the geomagnetic palaeosecular variation and relative palaeointensity of two parallel marine sedimentary cores drilled at the Fronte section at Taranto, Italy. U-channel samples were taken wherever core recovery and lithology permitted, producing a palaeomagnetic record at 1 cm resolution covering around 5 m of the total 9 m coring depth; discrete samples were taken from several of the intervals unsuitable for u-channelling.Stepwise alternating-field treatment resulted in good demagnetization behaviour, with most samples showing a clear, major origin-directed magnetization component. The average inclination of around 57° is close to the geocentric axial dipole inclination of 60° for the sampling site, with the difference attributable to typical inclination shallowing effects.Our record contains several brief excursions of shallowed inclination. The most significant of these reaches inclinations of below 10°, and we have tentatively correlated it with the Blake event. Relative palaeointensity exhibits a similarly dynamic behaviour across this interval. Integration of our palaeomagnetic record with ongoing biostratigraphic work, and with other regional palaeomagnetic data, will provide a more thorough characterization of Late Pleistocene palaeosecular variation in southern Europe.

  15. High-resolution mineral dust and sea ice proxy records from the Talos Dome ice core

    NASA Astrophysics Data System (ADS)

    Schüpbach, S.; Federer, U.; Kaufmann, P. R.; Albani, S.; Barbante, C.; Stocker, T. F.; Fischer, H.

    2013-12-01

    In this study we report on new non-sea salt calcium (nssCa2+, mineral dust proxy) and sea salt sodium (ssNa+, sea ice proxy) records along the East Antarctic Talos Dome deep ice core in centennial resolution reaching back 150 thousand years (ka) before present. During glacial conditions nssCa2+ fluxes in Talos Dome are strongly related to temperature as has been observed before in other deep Antarctic ice core records, and has been associated with synchronous changes in the main source region (southern South America) during climate variations in the last glacial. However, during warmer climate conditions Talos Dome mineral dust input is clearly elevated compared to other records mainly due to the contribution of additional local dust sources in the Ross Sea area. Based on a simple transport model, we compare nssCa2+ fluxes of different East Antarctic ice cores. From this multi-site comparison we conclude that changes in transport efficiency or atmospheric lifetime of dust particles do have a minor effect compared to source strength changes on the large-scale concentration changes observed in Antarctic ice cores during climate variations of the past 150 ka. Our transport model applied on ice core data is further validated by climate model data. The availability of multiple East Antarctic nssCa2+ records also allows for a revision of a former estimate on the atmospheric CO2 sensitivity to reduced dust induced iron fertilisation in the Southern Ocean during the transition from the Last Glacial Maximum to the Holocene (T1). While a former estimate based on the EPICA Dome C (EDC) record only suggested 20 ppm, we find that reduced dust induced iron fertilisation in the Southern Ocean may be responsible for up to 40 ppm of the total atmospheric CO2 increase during T1. During the last interglacial, ssNa+ levels of EDC and EPICA Dronning Maud Land (EDML) are only half of the Holocene levels, in line with higher temperatures during that period, indicating much reduced sea

  16. Systematic recover of long high-resolution rainfall time series recorded by pluviographs during the 20th century.

    NASA Astrophysics Data System (ADS)

    Delitala, Alessandro M. S.; Deidda, Roberto; Mascaro, Giuseppe; Piga, Enrico; Querzoli, Giorgio

    2010-05-01

    During most of the 20th century, precipitation has been continuously measured by means of the so-called "pluviographs", i.e. rain gauges including a mechanical apparatus for continuously recording the depth of water from precipitation on specific strip charts, usually on a weekly basis. The signal recorded on such strips was visually examined by trained personnel on a regular basis, in order to extract the daily precipitation totals and the maximum precipitation intensities over short periods (from a few minutes to hours). The rest of the high-resolution information contained in the signal was usually not extracted, except for specific cases. A systematic recovering of the entire information at high temporal resolution contained in these precipitation signals would provide a fundamental database to improve the characterization of historical rainfall climatology during the previous century. The Department of Land Engineering of the University of Cagliari has recently developed and tested an automatic software, based on image analysis techniques, which is able to acquire the scanned images of the pluviograph strip charts, to automatically digitise the signal and to produce a digital database of continuous precipitation records at the highest possible temporal resolution, i.e. 5 to 10 minutes. Along with that, a significant amount of daily precipitation totals from the late 19th and the 20th century, either elaborated from pluviograph strip charts or simply derived from bucket rain gauges, still exists in paper form, but it has never been digitalized. Within a project partly-funded by the Operational Programme of the European Union "Italia-Francia Marittimo", the Regional Environmental Protection Agency of Sardinia and the University of Cagliari will recover both the high-resolution rainfall signals and the older time series of daily totals recorded by a large number of pluviographs belonging to the historical monitoring networks of the island of Sardinia. Such data

  17. High-resolution patterning electronic polymers using dopant induced solubility control (Presentation Recording)

    NASA Astrophysics Data System (ADS)

    Moule, Adam J.; Jacobs, Ian E.; Li, Jun; Burg, Stephanie L.; Bilsky, David J.; Rotondo, Brandon T.; Stroeve, Pieter

    2015-08-01

    Organic electronics promise to provide flexible, large-area circuitry such as photovoltaics, displays, and light emitting diodes that can be fabricated inexpensively from solutions. A major obstacle to this vision is that most conjugated organic materials are miscible, making solution-based fabrication of multilayer or micro- to nanoscale patterned films problematic. Here we demonstrate that the solubility of prototypical conductive polymer poly(3-hexylthiophene) (P3HT) can be reversibly "switched off" using high electron affinity molecular dopants, then later recovered with light or a suitable dedoping solution. Using this technique, we are able to stack mutually soluble materials and laterally pattern polymer films using evaporation of dopants through a shadow mask or with light, achieving sub-micrometer, optically limited feature sizes. After forming these structures, the films can be dedoped without disrupting the patterned features; dedoped films have identical optical characteristics, charge carrier mobilities, and NMR spectra as as-cast P3HT films. This method greatly simplifies solution-based device fabrication, is easily adaptable to current manufacturing workflows, and is potentially generalizable to other classes of materials.

  18. Continuous zooming optical design with a high-resolution and large-zoom ratios used for precision strike TV camera

    NASA Astrophysics Data System (ADS)

    Wang, Ling; Chang, Weijun; Qiang, Hua; Zhang, Bo

    2015-02-01

    According to the requirement of detector, a continuous zooming TV camera with a high resolution and a large zoom ratios used for precision strike was designed. In this paper, basis the selection of continuous zooming optical systems was discussed. Combing with PW method, the incipient structure was computed. Using the CODE V, the optimum design was done. Having analyzed the cam curve of this zooming system, a continuous zooming optical system meeting the technical requirements well was designed, which provided the technical support for the miniaturization of the structure and the stability of the optic axis. This continuous zooming optical system has been checked with image quality testing, real imaging and environment testing and the result showed that the image quality was well, the optic axis was stable and the system meet the requirement of detector well.

  19. The 8.2 kyr event recorded at high resolution by a speleothem from the Northern French Alps

    NASA Astrophysics Data System (ADS)

    Couchoud, Isabelle; Drysdale, Russell; Hellstrom, John; Perrette, Yves

    2016-04-01

    Continental records of Holocene climate variability in the Alps are scarce and they rarely document the "8.2 kyr event". Several reasons have been proposed: records do not have sufficient resolution, are not precisely dated or the proxies are not sufficiently sensitive to climate variations associated with this event. The European records of adequate resolution generally allow the identification of an anomaly but its characterisation is unclear: it seems that the event has triggered significant impacts only for a few decades and mainly expressed in winter (whereas most archives are more sensitive to summer conditions). Thus, the detailed characterisation of the impacts of this event requires records with sub-annual resolution and preferably sensitive to winter conditions. Here we present a multi-proxy, high-resolution analysis of this event, as recorded by a stalagmite of the Bauges massif (Northern French Alps), at ~1400 m altitude. Comparison of these results with other regional and distant data allows for a discussion about climatic impacts and teleconnections at this time.

  20. Technical note: a new three-dimensional technique for high resolution quantitative recording of perikymata.

    PubMed

    Bocaege, E; Humphrey, L T; Hillson, S

    2010-03-01

    The number and spacing of incremental markings at the enamel surface, known as perikymata, are considered important indicators of dental growth patterns, as they provide information on crown formation times and the underlying developmental processes. This study explores the potential of a new three-dimensional technique for the reconstruction of dental growth profiles, using teeth from a medieval child from Abingdon, Oxfordshire. The crowns of three anterior teeth were imaged and analyzed using the Alicona 3D InfiniteFocus imaging microscope. Individual perikyma grooves can be unambiguously identified on a profile of the reconstructed enamel surface and direct distances between successive pairs of perikyma grooves can be calculated from coordinate data. This quantitative approach constitutes a more objective way to record perikymata spacing than current methods. PMID:19953528

  1. A high-resolution angiosperm pollen reference record covering Albian mid-latitude coastal deposits (Lusitanian Basin, Portugal)

    NASA Astrophysics Data System (ADS)

    Horikx, Maurits; Dinis, Jorge L.; Heimhofer, Ulrich

    2013-04-01

    The Lusitanian Basin in Portugal is one of the most important areas to investigate the rise and radiation of early angiosperms. Here, important micro-, macro- and mesofossil remains including pollen, reproductive organs, fruits and seeds have been found. In this study, a high-resolution Early to Late Albian pollen record from a thick (~160m) coastal succession in the Lusitanian Basin containing mixed carbonate-siliciclastic near-shore deposits is generated. The outcrop is located near the town of Ericeira (São Julião) and exhibits some important new features compared to existing records from the Lusitanian basin. The comparatively proximal depositional setting and high sedimentation rate of the São Julião outcrop is well suited for high-resolution palynological sampling compared to previously studied, more distal outcrops. In addition, the succession covers almost the entire Albian including a thick interval representing Late Albian strata. Dating of the succession was obtained using dinoflagellate cyst biostratigraphy, bulk C-isotope analysis and strontium isotope analysis of low-Mg oysters and rudist shells. The high-resolution pollen record shows a distinct radiation pattern of early angiosperm pollen as well as significant changes in the accompanying palynoflora. During most of the section gymnosperm pollen types such as Classopollis spp., Inaperturopollenites spp. and Exesipollenites spp. are dominant. Angiosperm pollen abundances do not exceed 20%, although angiosperms increase slightly from the Early Albian onwards. Monoaperturate grains of magnoliid or monocot affinity remain the most dominant angiosperm pollen type, both in abundances and diversity. Tricolpate and zonoaperturate pollen grains are also present. In addition, the occurrence of several odd-shaped Dichastopollenites-type pollen types is intriguing. The palynological results indicate a warm and dry climate during most of the Albian, although a rise in the spores over pollen ratio in the

  2. A computational approach to high-resolution imaging of the living human retina without hardware adaptive optics

    NASA Astrophysics Data System (ADS)

    Shemonski, Nathan D.; Adie, Steven G.; Liu, Yuan-Zhi; South, Fredrick A.; Carney, P. S.; Boppart, Stephen A.

    2015-03-01

    We demonstrate high-resolution imaging of the living human retina by computationally correcting highorder ocular aberrations. These corrections are performed post-acquisition and without the need for a deformable mirror or wavefront sensor that are commonly employed in hardware adaptive optics (HAO) systems. With the introduction of HAO to ophthalmic imaging, high-resolution near diffraction-limited imaging of the living human retina has become possible. The combination of a deformable mirror, wavefront sensor, and supporting hardware/software, though, can more than double the cost of the underlying imaging modality, in addition to significantly increasing the system complexity and sensitivity to misalignment. Optical coherence tomography (OCT) allows 3-D imaging in addition to naturally providing the complex optical field of backscattered light. This is unlike a scanning laser ophthalmoscope which measures only the intensity of the backscattered light. Previously, our group has demonstrated the utility of a technique called computational adaptive optics (CAO) which utilizes the complex field measured with OCT to computationally correct for optical aberrations in a manner similar to HAO. Until now, CAO has been applied to ex vivo imaging and in vivo skin imaging. Here, we demonstrate in vivo imaging of cone photoreceptors using CAO. Additional practical considerations such as imaging speed, and stability are discussed.

  3. Late Holocene climatic changes in west Africa, a high resolution diatom record from equatorial Cameroon

    NASA Astrophysics Data System (ADS)

    Nguetsop, Victor François; Servant-Vildary, Simone; Servant, Michel

    2004-03-01

    Holocene climatic changes in West Africa are usually explained by increased/decreased activity of the monsoon from the Guinean Gulf toward the continent. According to a diatom record from Lake Ossa (3°50'N, 9°36'E), we suggest that, in the near coastal areas of Cameroon, phases of intensification of the monsoon were marked by reduced precipitation and reduced evaporation, conditions nowadays prevailing South of the equator (4-5°S) during the austral winter. Lake Ossa is a shallow lake located in one of the rainiest area of the African rain forest belt. During the wet season (March-November) it is fed by acid meteoric waters entailing low pH in the lacustrine waters. During the dry season (December-February) groundwater discharges allow the persistence of acid waters near the borders of the lake, but, in the inner parts, the waters tend to be alkaline, alkaliphilous diatoms are abundant in the surface sediment samples and are used as indicators of low precipitation. At that time, atmospheric dust containing reworked diatoms from Saharan Quaternary deposits is transported by the northern trade winds and reaches the Ossa area. Wind blown diatoms are considered as a signature of the northern trade winds. A diatom record from the western deep part of Lake Ossa has provided climatic data for the mid-late Holocene at a resolution of 50-60 years. A major climatic change at 2700 cal yr BP was marked by the appearance of wind blown diatoms. A millennial-scale alternation between low and high precipitation episodes is recorded during the last 5500 years. The low precipitation episodes before 2700 cal yr BP are interpreted as a consequence of a northward extension of the climatic conditions that nowadays characterize the Southern Congo during the austral winter, when the monsoon extends into West Africa and reaches the northern sub-tropical latitudes. The effects of low precipitation on the water balance and on the rain forest were obliterated by an extremely low

  4. A high resolution record of chlorine-36 nuclear-weapons-tests fallout from Central Asia

    USGS Publications Warehouse

    Green, J.R.; Cecil, L.D.; Synal, H.-A.; Santos, J.; Kreutz, K.J.; Wake, C.P.

    2004-01-01

    The Inilchek Glacier, located in the Tien Shan Mountains, central Asia, is unique among mid-latitude glaciers because of its relatively large average annual accumulation. In July 2000, two ice cores of 162 and 167 meters (m) in length were collected from the Inilchek Glacier for (chlorine-36) 36Cl analysis a part of a collaborative international effort to study the environmental changes archived in mid-latitude glaciers worldwide. The average annual precipitation at the collection site was calculated to be 1.6 m. In contrast, the reported average annual accumulations at the high-latitude Dye-3 glacial site, Greenland, the mid-latitude Guliya Ice Cap, China, and the mid-latitude Upper Fremont Glacier, Wyoming, USA, were 0.52, 0.16 and 0.76 m, respectively. The resolution of the 36Cl record in one of the Inilchek ice cores was from 2 to 10 times higher than the resolution of the records at these other sites and could provide an opportunity for detailed study of environmental changes that have occurred over the past 150 years. Despite the differences in accumulation among these various glacial sites, the 36Cl profile and peak concentrations for the Inilchek ice core were remarkably similar in shape and magnitude to those for ice cores from these other sites. The 36Cl peak concentration from 1958, the year during the mid-1900s nuclear-weapons-tests period when 36Cl fallout was largest, was preserved in the Inilchek core at a depth of 90.56 m below the surface of the glacier (74.14-m-depth water equivalent) at a concentration of 7.7 ?? 105 atoms of 36Cl/gram (g) of ice. Peak 36Cl concentrations from Dye-3, Guliya and the Upper Fremont glacial sites were 7.1 ?? 105, 5.4 ?? 105 and 0.7 ?? 105 atoms of 36Cl/g of ice, respectively. Measurements of 36Cl preserved in ice cores improve estimates of historical worldwide atmospheric deposition of this isotope and allow the sources of 36Cl in ground water to be better identified. ?? 2004 Published by Elsevier B.V.

  5. High-Resolution Pollen Record of Deglacial Climate Variability in Central Florida

    NASA Astrophysics Data System (ADS)

    Willard, D. A.; Bernhardt, C. E.; Edgar, T.

    2004-12-01

    Pollen evidence from lacustrine sediments in Tampa Bay, Florida document considerable climatic variability superimposed on deglacial warming in the subtropics. Nine radiocarbon dates on well-preserved mollusk shells provide a reliable chronology of continuous sedimentation from 20 ka to 11.5 ka; examination of pollen assemblages from 2 cm increments within the lacustrine unit provide temporal resolution averaging one sample every 45 years. During the glacial maximum, much drier and cooler than modern conditions are indicated by pollen assemblages enriched in Chenopodiaceae, Carya, Poaceae and Ambrosia. Increased abundance of Pinus pollen between 17.4 ka and 15 ka indicates the existence of warmer, wetter conditions in the interval including Heinrich Event 1. After a reversion to drier, cooler conditions at about 15 ka, Pinus pollen abundance increased again at 14 ka. Combined with the near loss of Carya pollen, these data suggest attainment of nearly modern climatic conditions during the Bolling-Allerod (14-13 ka). However, variability within the Bolling-Allerod is documented by shifts to deglacial-equivalent vegetation during the Older Dryas (13.4-13.6 ka). The Younger Dryas (12.9-11.6 ka) is characterized by two distinct phases: from 12.9-11.9 ka, the increased abundance of Chenopodiaceae and Quercus mark drier, possibly cooler conditions comparable to those of the deglacial (17.4 - 14 ka). From 11.9-11.5 ka, strong dominance of herbaceous Chenopodiaceae is similar to full-glacial conditions, indicating much drier, possibly cooler conditions. Comparison of these paleoclimatic patterns with marine records from Orca Basin in the Gulf of Mexico indicate close temporal correspondence in warming of atmospheric and sea-surface temperatures. Pollen evidence also suggests that significant warming began around 17.4 ka, before the onset of Heinrich Event 1, as has been documented at other sites in the North Atlantic Ocean and Europe. This record of deglacial atmospheric

  6. Ultrasensitive high resolution laser spectroscopy and its application to optical frequency standards

    NASA Astrophysics Data System (ADS)

    Ye, Jun

    1997-09-01

    Advanced laser stabilization techniques now enable one to lock laser frequencies onto line centers of natural atomic/molecular resonances with unprecedented precision and accuracy. In this dissertation we discuss our effort in utilizing these techniques to establish visible optical frequency standards. By summarizing our earlier results on frequency measurements of the 87Rb D2 line at 780 nm 127I2 hyperfine transitions at 532 nm, we show the advantage of using a higher quality reference line, usually characterized by its narrower linewidth, higher attainable signal-to-noise ratio and lower sensitivity toward external perturbations. We then present a novel approach of cavity-enhanced frequency modulation spectroscopy for ultra-sensitive detections. The powerful utility of this new technique in the field of frequency standards is demonstrated by probing saturated molecular overtone transitions in the visible and near infrared. Weakly-absorbing gases such as C2H2 and C2HD are placed inside an external high-finesse resonator to enhance their detection sensitivities. A frequency modulation technique is employed to achieve a shot noise limited signal-to- noise ratio. The rf modulation frequency is chosen to match the cavity's free spectral range in order to avoid the cavity-induced conversion of laser frequency noise into amplitude noise. The molecular saturated dispersion signal is directly recovered after demodulation of the cavity transmitted light. A record high integrated absorption sensitivity of 5× 10-13/ (1× 10-14/cm) (at 1 second averaging time) has been obtained. Systematic studies on this new technique are presented on topics of detection sensitivity, signal line shape, signal size and slope, and pressure dependent linewidth broadening and linecenter shift. A Nd:YAG laser is stabilized on the P(5) transition in the (ν2+3/ ν3) overtone band of C2HD at 1.064 μm. Its absolute frequency is established. The excellent signal- to-noise ratio produces a frequency

  7. Speleothems as high-resolution paleoenvironment archives: Records from northeastern China

    NASA Astrophysics Data System (ADS)

    Ku, T. L.; Li, H. C.

    1998-12-01

    The isotopic compositions of oxygen and carbon and trace concentrations of magnesium and strontium in speleothems formed in limestone caves respond to climate changes outside the caves. Measurements of these properties on a stalagmite from Shihua Cave near Beijing, China, allowed reconstruction of the regional changes in precipitation, temperature and nature of vegetation. Over the last ˜ 500 years, there were fourteen precipitation cycles with a periodicity of 30-40 years, which may well reflect fluctuations in the strength of the East Asian summer monsoons reaching northeastern China. Relative to the mean temperature of this time interval, the period 1620-1900 AD was cold and periods 1520-1620 and 1900-1994 were warm. Over the last ˜ 3000-years, about eight wet/cool-dry/warm climatic cycles of 300-400 years duration occurred, the latest wet/cool half cycle corresponding to the Little Ice Age. The δ13C record registers the anthropogenic activities of fossil fuel CO2 combustion in recent decades and regional deforestation between 13 and 16 centuries when Beijing was bustling with palatial constructions and being developed into the world’s most populated city.

  8. Plio-Pleistocene climate sensitivity evaluated using high-resolution CO2 records.

    PubMed

    Martínez-Botí, M A; Foster, G L; Chalk, T B; Rohling, E J; Sexton, P F; Lunt, D J; Pancost, R D; Badger, M P S; Schmidt, D N

    2015-02-01

    Theory and climate modelling suggest that the sensitivity of Earth's climate to changes in radiative forcing could depend on the background climate. However, palaeoclimate data have thus far been insufficient to provide a conclusive test of this prediction. Here we present atmospheric carbon dioxide (CO2) reconstructions based on multi-site boron-isotope records from the late Pliocene epoch (3.3 to 2.3 million years ago). We find that Earth's climate sensitivity to CO2-based radiative forcing (Earth system sensitivity) was half as strong during the warm Pliocene as during the cold late Pleistocene epoch (0.8 to 0.01 million years ago). We attribute this difference to the radiative impacts of continental ice-volume changes (the ice-albedo feedback) during the late Pleistocene, because equilibrium climate sensitivity is identical for the two intervals when we account for such impacts using sea-level reconstructions. We conclude that, on a global scale, no unexpected climate feedbacks operated during the warm Pliocene, and that predictions of equilibrium climate sensitivity (excluding long-term ice-albedo feedbacks) for our Pliocene-like future (with CO2 levels up to maximum Pliocene levels of 450 parts per million) are well described by the currently accepted range of an increase of 1.5 K to 4.5 K per doubling of CO2. PMID:25652996

  9. Fabrication and characterization of a high-resolution neural probe for stereoelectroencephalography and single neuron recording.

    PubMed

    Pothof, F; Anees, S; Leupold, J; Bonini, L; Paul, O; Orban, G A; Ruther, P

    2014-01-01

    This paper reports on the design, fabrication, and characterization of neural probes for stereoelectroencephalography (SEEG). The probe specifically targets focal epilepsy as key application. However, probes of this type can also be used for the diagnosis and treatment of other neural dysfunctions such as Parkinson's disease or tremor, typically requiring deep brain probes. The probe fabrication, of which most processes are parallel batch processes, relies on a novel fabrication concept for rolling and gluing thin film polyimide sheets with integrated electrodes into permanent cylindrical shapes with diameters down to 800 μm. The SEEG probes, comprise several macro-electrodes designed to record local field potentials, and micro-electrodes positioned in-between, dedicated to monitoring single unit activity, with a total channel count of 32, despite the small diameter. While platinum micro-electrodes with a diameter of 35 μm have impedances of about 255 kΩ at 1 kHz, impedance values down to about 1.5 kΩ have been measured for the macro-electrodes. The devices have shown good compatibility with magnetic resonance imaging in a 9.4 T magnet, enabling the precise post-operative probe localization within the brain. PMID:25571176

  10. Note: Autocollimation with ultra-high resolution and stability using telephoto objective together with optical enlargement and beam drift compensation.

    PubMed

    Zhu, Fan; Tan, Xinran; Tan, Jiubin; Fan, Zhigang

    2016-08-01

    An autocollimation (AC) setup with ultra-high resolution and stability for micro-angle measurement is presented. The telephoto objective, which is characterized in long focal length at a compact structure size, and the optical enlargement unit, which can magnify the image displacement to improve its measurement resolution and accuracy, are used to obtain an ultra-high measurement resolution of the AC. The common-path beam drift compensation is used to suppress the drift of measurement results, which is evident in the high-resolution AC, thus to obtain a high measurement stability. Experimental results indicate that an effective resolution of better than 0.0005 arc sec (2.42 nrad) over a measurement range of ±30 arc sec and a 2-h stability of 0.0061 arc sec (29.57 nrad) can be achieved. PMID:27587181

  11. Fabrication of high-resolution reflective scale grating for an optical encoder using a patterned self-assembly process

    NASA Astrophysics Data System (ADS)

    Fan, Shanjin; Jiang, Weitao; Li, Xuan; Yu, Haoyu; Lei, Biao; Shi, Yongsheng; Yin, Lei; Chen, Bangdao; Liu, Hongzhong

    2016-07-01

    Steel tape scale grating of a reflective incremental linear encoder has a key impact on the measurement accuracy of the optical encoder. However, it is difficult for conventional manufacturing processes to fabricate scale grating with high-resolution grating strips, due to process and material problems. In this paper, self-assembly technology was employed to fabricate high-resolution steel tape scale grating for a reflective incremental linear encoder. Graphene oxide nanoparticles were adopted to form anti-reflective grating strips of steel tape scale grating. They were deposited in the tape, which had a hydrophobic and hydrophilic grating pattern when the dispersion of the nanoparticles evaporated. A standard lift-off process was employed to fabricate the hydrophobic grating strips on the steel tape. Simultaneously, the steel tape itself presents a hydrophilic property. The hydrophobic and hydrophilic grating pattern was thus obtained. In this study, octafluorocyclobutane was used to prepare the hydrophobic grating strips, due to its hydrophobic property. High-resolution graphene oxide steel tape scale grating with a pitch of 20 μm was obtained through the self-assembly process. The photoelectric signals of the optical encoder containing the graphene oxide scale grating and conventional scale grating were tested under the same conditions. Comparison test results showed that the graphene oxide scale grating has a better performance in its amplitude and harmonic components than that of the conventional steel tape scale. A comparison experiment of position errors was also conducted, demonstrating an improvement in the positioning error of the graphene oxide scale grating. The comparison results demonstrated the applicability of the proposed self-assembly process to fabricate high-resolution graphene oxide scale grating for a reflective incremental linear encoder.

  12. High-Resolution Pleistocene Alkenone Temperature Records of IODP Expedition 346 Sites U1425 and U1429

    NASA Astrophysics Data System (ADS)

    Lee, K. E.; Bae, S. W.; Kim, K.; Kim, R. A.; Kang, N.; Ko, T. W.; Tada, R.; Murray, R. W.; Alvarez Zarikian, C. A.

    2014-12-01

    Site U1425 drilled by IODP Expedition 346 is located at the top of the Yamato Ridge, the central part of the sea. This study reconstructs variations in SST for the last 2 million years by using the alkenone unsaturation index of marine sediments recovered from Site U1425. There have been many studies on the palaeoceanography of the northwestern Pacific marginal seas covering the last glacial maximum and/or the penultimate glacial maximum periods. Until recently, however, little information was available on longer time scale proxy records of the northwestern Pacific margin. Establishing palaeoceanographic records from the northwestern Pacific margin is necessary for understanding the large scale ocean and climate changes and/or their linkages. This study presents a long term alkenone temperature record of Site U1425 spanning the last two million years. The reconstructed alkenone temperatures clearly illustrate the variability being consistent with orbital-scale global climate records over the period. Especially the alkenone SSTs fluctuate greatly and they are comparable to world-wide benthic foraminiferal isotope stack record. We will present more details regarding the characteristics of 2 million year SSTs in the presentation. Site U1429 was drilled by IODP Expedition 346 in the northern part of the Okinawa Trough in the East China Sea. Here we will also present extremely high-resolution alkenone temperature changes of Site U1429 over the last 400,000 years. Comparison of these records with other proxy records from high- to low-latitudes will give us a unique chance to understand the linkage between latitudinal climate patterns and dynamics.

  13. A New Method of Obtaining High-Resolution Paleoclimate Records from Speleothem Fluid Inclusions

    NASA Astrophysics Data System (ADS)

    Logan, A. J.; Horton, T. W.

    2010-12-01

    a minimum quantity of ~100nL water was required. In contrast to liquid water analyses, carbonate inclusion waters gave highly variable results. As plenty of signal was produced from relatively small sample sizes (~20 mg), the observed isotopic variation most likely reflects fractionation during fluid extraction, or natural isotopic variability. Additional tests and modifications to the extraction procedure are in progress, using a recently collected New Zealand stalagmite from a West Coast cave (DOC collection permit WC-27462-GEO). U-Th age data will accompany a paleoclimate record from this stalagmite obtained using standard carbonate analytical techniques, and compared to the results from our new fluid inclusion analyses.

  14. High resolution in-vivo imaging of skin with full field optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Dalimier, E.; Bruhat, Alexis; Grieve, K.; Harms, F.; Martins, F.; Boccara, C.

    2014-03-01

    Full-field OCT (FFOCT) has the ability to provide en-face images with a very good axial sectioning as well as a very high transverse resolution (about 1 microns in all directions). Therefore it offers the possibility to visualize biological tissues with very high resolution both on the axial native view, and on vertical reconstructed sections. Here we investigated the potential dermatological applications of in-vivo skin imaging with FFOCT. A commercial FFOCT device was adapted for the in-vivo acquisition of stacks of images on the arm, hand and finger. Several subjects of different benign and pathological skin conditions were tested. The images allowed measurement of the stratum corneum and epidermis thicknesses, measurement of the stratum corneum refractive index, size measurement and count of the keratinocytes, visualization of the dermal-epidermal junction, and visualization of the melanin granules and of the melanocytes. Skins with different pigmentations could be discriminated and skin pathologies such as eczema could be identified. The very high resolution offered by FFOCT both on axial native images and vertical reconstructed sections allows for the visualization and measurement of a set of parameters useful for cosmetology and dermatology. In particular, FFOCT is a potential tool for the understanding and monitoring of skin hydration and pigmentation, as well as skin inflammation.

  15. Hypoxia in high-resolution sediment records: reconstructing the California Current Oxygen Minimum Zone on multi-decadal timescales

    NASA Astrophysics Data System (ADS)

    Moffitt, S. E.; Hill, T. M.

    2012-12-01

    The recent deglaciation event is an ideal laboratory to study the rapid expansion of Oxygen Minimum Zones (OMZs) and the ecological ramifications of such events. Santa Barbara Basin (SBB) sediments are high-resolution archives of seafloor ecosystems, recording both global-scale climate and regional-scale hydrographic events. Seafloor hypoxia in the California Current Ecosystem (CCE) is caused by OMZs in intermediate water depths (300-1200 m), and produces striking evidence in SBB sediment archives. We construct a vertical transect of proxies across SBB (34° 15'N, 119° 45'W) using a core from 418 m water depth (MV0811-15JC), and previously investigated cores from 440 m (MD02-2504) and 570 m (MD02-2503) water depths. Benthic foraminiferal assemblages and planktonic δ18O proxies were quantified at all three depths, while benthic invertebrate communities were quantified in the shallowest core at a 1-cm resolution (~10 years). This high-resolution invertebrate record provides a window into rapid, decadal-scale environmental change in continental margin ecosystems. Seafloor biodiversity is highly variable on 10-10^4 year timescales (across Protist, Mollusc, Arthropod and Echinoderm taxonomic groups), and is tightly coupled to both regional-scale environmental change and global-scale climate events. Additionally, we provide evidence that strongly hypoxic waters shoaled to <300 m water depth at Termination 1A (14.7 ka), implying that the upper boundary of the regional OMZ can expand >150 m on multi-decadal timescales. These data confirm that OMZs have rapidly expanded in the CCE during previous events of global-scale warming, and that continental margin seafloor biodiversity is variable on previously undescribed timescales.

  16. A conceptual design for a Cassegrain-mounted high-resolution optical spectrograph for large-aperture telescopes

    NASA Astrophysics Data System (ADS)

    Froning, Cynthia S.; Osterman, Steven; Burgh, Eric; Beasley, Matthew; Scowen, Paul; Veach, Todd; Jordan, Steven; Ebbets, Dennis; Lieber, Michael; deCino, James; Castilho, Bruno Vaz; Gneiding, Clemens; César de Oliveira, Antonio

    2013-09-01

    We present a conceptual design for a high-resolution optical spectrograph appropriate for mounting at Cassegrain on a large aperture telescope. The design is based on our work for the Gemini High Resolution Optical Spectrograph (CUGHOS) project. Our design places the spectrograph at Cassegrain focus to maximize throughput and blue wavelength coverage, delivering R=40,000 resolving power over a continuous 320-1050 nm waveband with throughputs twice those of current instruments. The optical design uses a two-arm, cross-dispersed echelle format with each arm optimized to maximize efficiency. A fixed image slicer is used to minimize optics sizes. The principal challenge for the instrument design is to minimize flexure and degradation of the optical image. To ensure image stability, our opto-mechanical design combines a cost-effective, passively stable bench employing a honeycomb aluminum structure with active flexure control. The active flexure compensation consists of hexapod mounts for each focal plane with full 6-axis range of motion capability to correct for focus and beam displacement. We verified instrument performance using an integrated model that couples the optical and mechanical design to image performance. The full end-to-end modeling of the system under gravitational, thermal, and vibrational perturbations shows that deflections of the optical beam at the focal plane are <29 μm per exposure under the worst case scenario (<10 μm for most orientations), with final correction to 5 μm or better using open-loop active control to meet the stability requirement. The design elements and high fidelity modeling process are generally applicable to instruments requiring high stability under a varying gravity vector.

  17. Retinal Structure of Birds of Prey Revealed by Ultra-High Resolution Spectral-Domain Optical Coherence Tomography

    PubMed Central

    Ruggeri, Marco; Major, James C.; McKeown, Craig; Knighton, Robert W.; Puliafito, Carmen A.

    2010-01-01

    Purpose. To reveal three-dimensional (3-D) information about the retinal structures of birds of prey in vivo. Methods. An ultra-high resolution spectral-domain optical coherence tomography (SD-OCT) system was built for in vivo imaging of retinas of birds of prey. The calibrated imaging depth and axial resolution of the system were 3.1 mm and 2.8 μm (in tissue), respectively. 3-D segmentation was performed for calculation of the retinal nerve fiber layer (RNFL) map. Results. High-resolution OCT images were obtained of the retinas of four species of birds of prey: two diurnal hawks (Buteo platypterus and Buteo brachyurus) and two nocturnal owls (Bubo virginianus and Strix varia). These images showed the detailed retinal anatomy, including the retinal layers and the structure of the deep and shallow foveae. The calculated thickness map showed the RNFL distribution. Traumatic injury to one bird's retina was also successfully imaged. Conclusions. Ultra-high resolution SD-OCT provides unprecedented high-quality 2-D and 3-D in vivo visualization of the retinal structures of birds of prey. SD-OCT is a powerful imaging tool for vision research in birds of prey. PMID:20554605

  18. A high-resolution, absolute-dated deglacial speleothem record of Indian Ocean climate from Socotra Island, Yemen

    NASA Astrophysics Data System (ADS)

    Shakun, Jeremy D.; Burns, Stephen J.; Fleitmann, Dominik; Kramers, Jan; Matter, Albert; Al-Subary, Abdulkarim

    2007-07-01

    Stalagmite M1-5 from Socotra Island, Yemen in the northwest Indian Ocean provides a robust, high-resolution paleoclimate record from ˜ 27.4-11.1 ka based on 717 stable isotope and 28 230Th measurements. Variations in M1-5 oxygen isotope ratios ( δ18O) are interpreted to be primarily driven by an amount effect related to changes in the mean position and/or intensity of convection of the intertropical convergence zone, the island's only source of precipitation. The M1-5 δ18O time series is strongly correlated to the Greenland ice cores, similar to an older Socotra speleothem deposited from 53-40 ka [S.J. Burns, D. Fleitmann, A. Matter, J. Kramers, A. Al-Subbary, Indian Ocean climate and an absolute chronology over Dansgaard/Oeschger events 9 to 13, Science 301 (2003) 1365-1367], indicating that a North Atlantic-Indian Ocean cold-dry/warm-wet teleconnection persisted through the end of the last glacial period. Peak aridification occurred at ˜ 23 ka and a gradual increase in moisture thereafter was interrupted by an abrupt drying event at ˜ 16.4 ka, perhaps related to Heinrich event 1. Indian Ocean rainfall increased dramatically during the Bølling period and then decreased continuously and gradually through the Allerød and Younger Dryas. The Holocene began abruptly with increased precipitation at 11.4 ka and was followed by a major but short-lived drying during the Preboreal Oscillation at ˜ 11.2 ka. M1-5 is highly correlated to the Dongge Cave record from 15.5-11 ka, suggesting much of the Indian Ocean monsoon region responded similarly to the major climate changes of the last deglaciation. The transitions into the Younger Dryas and to a lesser extent the Bølling are remarkably gradual in M1-5, as they are in all other Asian speleothem records, occurring over several centuries. These gradual transitions are in striking contrast to high-resolution records from around the North Atlantic basin where the transitions are extremely abrupt and generally occur in

  19. A High-Resolution Stalagmite Record of East-Central North America Hydroclimates during Marine Isotope Stages 3-5

    NASA Astrophysics Data System (ADS)

    Springer, G. S.; Rowe, H. D.; Hardt, B. F.; Cheng, H.; Edwards, R. L.

    2014-12-01

    Long-term, high-resolution stalagmite carbon and oxygen isotope records from eastern North America (ENA) provides a mid-latitude history of relative changes in moisture availability and climate states during the last interglacial and glacial inception (127.7 to 41.6 ka before present). The West Virginia carbon record (δ13C) shows low-amplitude variability at orbital time scales, superimposed on a long-term asymmetric pattern similar to global sea level changes. Relative moisture availability peaked at ~114ka and following a brief dry interval at ~96ka, moisture availability gradually decreased. The gradual changes in moisture availability over ENA may reflect similarly gradual changes in mid-latitude zonal circulation as the polar cell and Laurentide Ice Sheet expanded or decreased. However, high frequency isotopic fluctuations are present and correlative with climatic phenomena recorded in Greenland. In contrast to the gradual changes in carbon isotopes, our oxygen record (δ18O) is precession-modulated and in phase with spring insolation, perhaps due to changes in precipitation seasonality. Altered precipitation seasonality or seasonal moisture availabilities would, as a result of annual variability in meteoric δ18O, have caused a weighting effect in stalagmitic calcite precipitation. However, this explanation for changes in δ18O does not explain why the two isotopic records of eccentricity (carbon) and precession (oxygen) are paced differently because moisture availability might resonably be expected to covary with precipitation seasonality. The same pattern is observed in a stalagmite from the previous interglacial-glacial cycle, so it is a persistent feature in our study area. We will present possible explanations.

  20. High-resolution CCD imagers using area-array CCD's for sensing spectral components of an optical line image

    NASA Technical Reports Server (NTRS)

    Elabd, Hammam (Inventor); Kosonocky, Walter F. (Inventor)

    1987-01-01

    CCD imagers with a novel replicated-line-imager architecture are abutted to form an extended line sensor. The sensor is preceded by optics having a slit aperture and having an optical beam splitter or astigmatic lens for projecting multiple line images through an optical color-discriminating stripe filter to the CCD imagers. A very high resolution camera suitable for use in a satellite, for example, is thus provided. The replicated-line architecture of the imager comprises an area-array CCD, successive rows of which are illuminated by replications of the same line segment, as transmitted by respective color filter stripes. The charge packets formed by accumulation of photoresponsive charge in the area-array CCD are read out row by row. Each successive row of charge packets is then converted from parallel to serial format in a CCD line register and its amplitude sensed to generate a line of output signal.

  1. A high-resolution compact optical true-time delay beamformer using fiber Bragg grating and highly dispersive fiber

    NASA Astrophysics Data System (ADS)

    Gao, Xinlu; Huang, Shanguo; Wei, Yongfeng; Gao, Chao; Zhou, Jing; Zhang, Hanyi; Gu, Wanyi

    2014-10-01

    A high resolution optical true-time delay (OTTD) beamformer constructed by fiber Bragg grating (FBG) and highly dispersive fiber (HDF) is presented. It can produce the true time delay with the resolution of 1 ps. Besides the proposed system has compact structure and light weight even when a large number of antenna elements are present in a practical antenna array, this is because the used FBG fibers and HDFs are short and independent of the antenna element number. Theoretical analysis and numerical simulations are made. Proof-of-concept experiment results that demonstrate the feasibility of the system are presented.

  2. High-resolution elemental records of Glycymeris glycymeris (Bivalvia) shells from the Iberian upwelling system: Ontogeny and environmental control

    NASA Astrophysics Data System (ADS)

    Freitas, Pedro; Richardson, Christopher; Chenery, Simon; Butler, Paul; Reynolds, David; Gaspar, Miguel; Scourse, James

    2015-04-01

    The great potential of bivalve shells as a high-resolution geochemical proxy archive of environmental conditions at the time of growth has been known for several decades. The elemental composition of bivalve shells has been studied with the purpose of reconstructing environmental conditions: e.g. seawater temperature (Sr and Mg), primary productivity (Li, Mn, Mo and Ba), redox conditions (Mn and Mo), terrigenous inputs (Li) and pollution (Cu, Zn, Cd and Pb). However, the interpretation of such records remains extremely challenging and complex, with processes affecting element incorporation in the shell (e.g. crystal fabrics, organic matrix, shell formation mechanisms and physiological processes) and the influence of more than one environmental parameter affecting elemental composition of bivalve shells. Nevertheless, bivalve shells remain an underused source of information on environmental conditions, with the potential to record high-resolution (sub-weekly to annually), multi-centennial time series of geochemical proxy data. The relatively long-lived bivalve (>100 years) Glycymeris glycymeris occurs in coastal shelf seas of Europe and North West Africa and is a valid annually resolved sclerochronological archive for palaeonvironmental reconstructions. The temporal framework provided by absolute annually dated shell material makes Glycymeris glycymeris a valuable, albeit unexplored, resource for investigating sub-annually resolved geochemical proxies. We present a first evaluation on the potential of Ba, P and U, the latter two elements rarely studied in bivalves, in Glycymeris glycymeris shells to record variations in the environmental conditions, respectively primary productivity, dissolved inorganic phosphorus and carbonate ion concentration/pH. High-resolution (31 to 77 samples per year) profiles of elemental/Ca ratios (E/Ca) over four years of growth (2001 to 2004) were obtained by LA-ICP-MS on two shells (13 and 16 years old) live-collected in 2010 at 30 m

  3. High-resolution record of Early to Middle Miocene climate variability from Site 1195, Marion Plateau, NE Queensland margin

    NASA Astrophysics Data System (ADS)

    Lowery, C.; Browning, E.; Leckie, R. M.; John, C. M.

    2012-12-01

    Constraining and quantifying eustatic variations has been a priority for stratigraphers since the publication of the first global sea level curves by the Exxon research group in the late 1970s. Quantifying the glacio-eustatic component of sea level signals has become a greater focus as more recent work has demonstrated that far-field effects like ice-sheet gravitation and isostasy imprint on the "true" eustatic signal of waxing and waning ice sheets so that coeval signals from disparate sites may show significantly different local sea level variations. To this end, the Miocene sediments on the Marion Plateau, a drowned carbonate platform on the Queensland margin of Australia, were drilled by ODP Leg 194 to provide an independent, southern hemisphere test of the sea level record of the New Jersey Margin of North America, the most complete and oft-cited record of Cenozoic sea level variability. A high-resolution record is critical to compare sea level variations across hemispheres. Natural Gamma Ray (NGR) logs of core holes can provide a complete, high-resolution record independent of any problems with core recovery or sampling interval. We here present a NGR record of ODP Site 1195 tied to recently completed nannofossil assemblage data, planktic/benthic foraminiferal ratios, sedimentary particle counts, and benthic foraminifera stable isotopes. Peaks in glauconite and clay content correspond to peaks in NGR. The largest of these peaks correspond to sequence boundaries (lowstands), as glauconite accumulates during periods of low sedimentation along this carbonate-dominated margin. These sequence boundaries, in turn, are each associated with marine oxygen isotope events ("Mi Events") and correlate to sequences on the New Jersey margin, the Gulf of Papua, Great Australian Bight, and McMurdo Sound, indicating that these sequences are eustatically controlled. Although sedimentary particle counts only show strong peaks of glauconite at sequence boundaries, the NGR record

  4. Resolving seasonality in tropical trees: multi-decade, high-resolution oxygen and carbon isotope records from Indonesia and Thailand

    NASA Astrophysics Data System (ADS)

    Poussart, Pascale F.; Evans, Michael N.; Schrag, Daniel P.

    2004-02-01

    Dendrochronological techniques have found limited applications in the tropics because of invisible or indistinct banding in wood. The seasonal cycles of rainfall and relative humidity in these regions, while not sufficient to produce distinct visible rings, may still generate seasonal signals in the oxygen isotopic composition of tree cellulose which can be used for climate reconstruction and chronology. We explore this approach using trees from Indonesia and Thailand, from three different families. Multi-decade δ 18O records from Javanese cross-dated teak rings and bomb radiocarbon-dated Suar wood lacking visible rings demonstrate the reproducibility of the signal between trees grown at the same locality as well as from wider geographical regions. These results confirm predictions that the trees oxygen isotopic signatures reflect an external climatic forcing. High-resolution δ 18O records reveal large seasonal cycles: up to 4‰ for Javanese Suar samples and up to 18‰ for a Thai Podocarpus sample. We show that the six δ 18O and δ 13C cycles measured on a Podocarpus match the number of growth years for the period spanning the time of wounding and cutting of the wedge section. This result demonstrates that the isotopic cycles found in this tree with indistinct annual rings are indeed seasonal and could be used for chronology. We present evidence that stable isotope chronologies of tropical trees also contain insights in tropical tree physiology and growth dynamics.

  5. A high-resolution measurement device for detecting the positioning accuracy of the optical fiber positioner

    NASA Astrophysics Data System (ADS)

    Gu, Yonggang; Xu, Jianlei; Jin, Yi; Zhai, Chao

    2012-09-01

    The optical fiber positioner with double revolving mechanism is driven by two stepping motors. One stepping motor drives center revolving mechanism and the other drives decentered slewing mechanism. Photogrammetry is currently used to detect the positioning accuracy of the optical fiber positioner, but it cannot achieve high precision because of the small size of the fiber’s diameter. So, a new measurement device, which mainly contained optical microscope, CCD camera and two-dimensional precision mobile platform, was established in this paper. One end of the optical fiber (the other end was lighted by integrating sphere light source) was imaged on the CCD sensor in a magnified way through the optical microscope, and the image was processed to build the position feedback mechanism in real time. Then the two-dimensional mobile platform was controlled by PID control method to track the optical fiber, and the fiber was always kept to locate in center of the CCD image in order to eliminate the aberrations of the optical microscope lens. Finally, the position changes of the moving fiber could be obtained by the coordinates of the two-dimensional precision mobile platform. The experimental results demonstrate that the resolution of this measurement device is 0.1μm and the accuracy of repeat positioning is 1.5μm. The measurement device could satisfy the testing requirement.

  6. Permanent Electronic Storage of Angiographic Images in the Catheterization Laboratory: High-Resolution and Real-Time Acquisition on Optical Laserdiscs.

    PubMed

    Azancot; Krone; Eiferman; Guermonprez; Metzger; Le Dref O; Steg; Bonan; Lesperance; Hudon; Friolo; Beaufils

    1997-03-01

    The use of electronic ÒfilmlessÓ media for long-term archiving of coronary angiograms has been impeded by the problems of image storage and data transfer among institutions. Although long-term analogue storage of the images is presently feasible, and much less costly than digital storage, processing has been limited to a 625 lines video format, not optimal for high quality images. We developed a bi-directional 1249/625 lines converter, able to store and to replay high resolution (1249 lines) video images, from Component Record Video (CRV) Optical Laser (Analogue) Videodisks. Image quality and medical relevance were evaluated five ways: 1) Outside experts compared 593 static images stored on CRV discs to the same images stored in a high-resolution digital format blinded to source of image. The four experts found no visual or medical difference in 98% of evaluated images and minor differences in the remainder. The differences in the remaining images were not consistent among experts. 2) Two of the experts also compared the enhanced CRV optically stored image to the image obtained on simultaneously recorded 35mm cine film, and found the enhanced CRV stored image to be superior or similar, but never inferior to the film image. 3) 90 representatives from 63 outside institutions compared images from both a digital hard drive and the enhanced CRV optical (analogue) storage displayed at a Windows based digital workstation. During the test they were blinded as to the source of the images. The representatives found no difference in image resolution, quality, diagnostic accuracy, and medical relevance. 4) We evaluated quantitative coronary angiography (QCA) on standard coronary test phantoms using enhanced CRV stored images digitally processed. The correlation of the enhanced CRV image to the actual size of the phantom vessels was similar to the results obtained in the literature from digitally stored images. 5) 78 arterial measurements ranging 0.65 to 4.85 mm were

  7. Forbidden lines of (O I) in the high-resolution optical spectra of planetary nebulae

    NASA Astrophysics Data System (ADS)

    Keenan, F. P.; Aller, L. H.; Hyung, S.; Brown, P. J. F.

    1995-02-01

    Electron impact excitation rates for transitions in O I, calculated with the R-matrix code, are used to derive the electron-temperature sensitive emission-line ratio R = I(2s22p4 (1D) -2s22p4 1S/2s22p4 (3P)1,2-2s22p4 1D = I(5577 A)/I(6300+6365 A), for a range of electron temperatures Te = 5000-20 000 K) and densities (ne = 104 - 106/cu cm) applicable to planetary nebulae. Experimental values of R for a number of planetaries have been measured from high-resolution (approximately 0.6 A FWHM) spectra obtained with the Hamilton Echelle spectrograph on the 3-m telescope at the Lick Observatory. These measurements should be particularly reliable, as the sample of planetaries was restricted to those with large enough radial velocities for the nebular (O I) 5577 A emission to be red- or blue-shifted from the atmospheric airglow feature by a sufficient amount for the former to be reliably determined. Electron temperatures deduced from the observed values of R are generally in good agreement with those derived from Te-sensitive line ratios in other species, providing observational support for the accuracy of the atomic data adopted in the calculations.

  8. High Resolution, High Throughput X-Ray Observatory with Adjustable Optics

    NASA Astrophysics Data System (ADS)

    Vikhlinin, Alexey; Brissenden, R.; Bookbinder, J.; Cotroneo, J.; Davis, W.; Forman, W. R.; Freeman, M.; Murray, S. S.; O'Dell, S.; Ramsey, B.; Romaine, S.; Schwartz, D.; Tananbaum, H.; Trolier-McKinstry, S.; Wilke, R. H. T.

    2011-09-01

    An X-ray telescope with sub-arcsec angular resolution and >104 cm2 effective area will trigger another revolution in high energy astrophysics. We are developing technologies which would make such a telescope possible: adjustable grazing incidence bimorph mirrors composed of thin glass or metal segments with a few micrometer layer of piezoelectric material deposited on the back surface. The piezo cells are used to correct mirror figure errors due to fabrication, mounting, gravity release, and thermal deformations. Supporting analytical studies show the feasibility of mirror control, and laboratory demonstrations have shown that displacements of sufficient amplitude can be produced to achieve the required correction. The science topics for a high-resolution, large area X-ray telescope range from neutron star binary populations in the Milky Way and nearby galaxies to studies of diffuse baryons in the biggest objects in the Universe and in the warm-hot intergalactic medium. There will be a significant time span relative to the Chandra baseline for detailed observations of the expanding SNRs, reflection of the past activity of Sgr A* from surrounding molecular clouds, cooling of isolated neutron stars, etc. An exciting new frontier is observations of co-evolution of the supermassive black holes and their host galaxies across the peak in the cosmic star formation at z 3 and into the end of Dark Ages at z=6-10.

  9. High Resolution deglacial monsoon δ18O record from a new stalagmite from the Kailash Cave, Central India

    NASA Astrophysics Data System (ADS)

    Allu C, Narayana; Pawan K, Gautam; Shraddha, Band; Madhusudan G, Yadava; Rengaswamy, Ramesh; Shen, Chuan-Chou

    2016-04-01

    High resolution δ18O and δ13C data from absolutely dated stalagmites have been useful for reconstructing the Asian monsoon variability (e.g., Yadava et al., 2004; Laskar et al., 2013; Allu et al., 2014; Lone et al., 2014; Sinha et al., 2015). However, many studies lack high resolution spatial and temporal records leaving significant gaps which need to be filled for a vivid understanding of monsoonal variability. We report here the first high resolution stalagmite δ18O isotope results during the last deglacial obtained from the Kailash cave located from the core monsoon region. The length of stalagmite was 480 mm, with an average diameter of 120 mm. The sample was cut for continuous micro milling at 400μm intervals along the growth axis (using new wave research micro-mill-101288) for the analyses of stable oxygen and carbon isotopes using a Delta V plus IRMS at the Physical Research Laboratory, Ahmedabad. The physical appearance of the sample section reveals very fine, straight and clear laminations from the top to 310 mm from below, which have thick laminae. U-Th dates obtained from a Thermo Fisher NEPTUNE multi-collector inductively coupled plasma mass spectrometer (MC-ICP-MS) at High-Precision Mass Spectrometry and Environment Change Laboratory (HISPEC), National Taiwan University, Taiwan (Shen et al., 2012) showed the record spanned ~2400 years from ~14.6 ka to ~12.2 ka. Linear Age-Depth model constructed from dates suggests that the sample grew for ~2.400 years from ~14.6 ka to ~12.2 ka with varying resolutions from ~6 months to ~8 years. Hendy's test from 8 distinct layers shows poor correlation between δ18O and δ13C suggesting the isotopic equilibrium conditions at the time of crystallization. δ18O and δ13C results appear to be cyclic in nature varying in the range from +0.37‰ to -6.07‰ and -1.59‰ to -10.59‰ respectively. Enriched δ18O in top portion represents poor monsoon during the onset of Younger Drayas. Later, the δ18O signals

  10. A novel high-resolution optical imaging modality: photo-magnetic imaging

    NASA Astrophysics Data System (ADS)

    Luk, Alex T.; Thayer, David; Lin, Yuting; Nouizi, Farouk; Gao, Hao; Gulsen, Gultekin

    2013-03-01

    We introduce an entirely new technique, termed Photo-Magnetic Imaging (PMI), which overcomes the limitation of pure optical imaging and provides optical absorption at MRI spatial resolution. PMI uses laser light to heat the medium under investigation and employs MR thermometry for the determination of spatially resolved optical absorption in the probed medium. A FEM-based PMI forward solver has been developed by modeling photon migration and heat diffusion in tissue to compare simulation results with measured MRI maps. We have successfully performed PMI using 2.5 cm diameter agar phantom with two low optical absorption contrast (x 4) inclusions under the ANSI limit. Currently, we are developing the PMI inverse solver and undertaking further phantom and in vivo experiments.

  11. An integrable high resolution all-optical analog-to-digital conversion scheme

    NASA Astrophysics Data System (ADS)

    Wei, Shile; Jian, Wu; Zhao, Lingjuan; Lu, Dan; Qiu, Jifang

    2014-05-01

    A novel 4 × 4 multimode interference couplers based phase-shifted photonic quantization scheme using multiwavelength mode locked pulse lasers as sampling source for all-optical analog-to-digital converter is proposed. Numerical analysis indicates that 8-bit quantization resolution operating at 40 GHz bandwidth could be achieved with an incident average optical power of 1.932 mW to each photodiode. The whole scheme can be integrated on a InP-based chip.

  12. High-Resolution Broadband Millimeter-Wave Astrophysical Spectrometer with Triple Product Acousto-Optical Processor

    NASA Astrophysics Data System (ADS)

    Dagostino, Miguel Chavez; Shcherbakov, Alexandre S.; Arellanes, Adan Omar; Chavushyan, Vahram

    An advanced conceptual design of a high-bit-rate triple product acousto-optical processor is presented that can be applied in a number of astrophysical problems. We briefly describe the Large Millimeter Telescope as one of the potential observational infrastructures where the acousto-optical spectrometer can be successfully used. A summary on the study of molecular gas in relatively old (age > 10 Myr) disks around main sequence stars is provided. We have identified this as one of the science cases in which the proposed processor can have a big impact. Then we put forward triple product acousto-optical processor is able to realize algorithm of the space-and-time integrating, which is desirable for a wide- band spectrum analysis of radio-wave signals with an improved resolution providing the resolution power of about 105 - 106. It includes 1D-acousto-optic cells as the input devices for a 2D-optical data processing. The importance of this al- gorithm is based on exploiting the chirp Z-transform technique providing a 2D-Fourier transform of the input signals. The system produces the folded spectrum, accumulating advantages of both space and time integrating. Its frequency bandwidth is practically equal to the bandwidth of transducers inherent in acousto-optical cells. Then, similar processor is able to provide really high frequency resolution, which is practically equal to the reciprocal of the CCD-matrix photo-detector integration time. Here, the current state of developing the triple product acousto-optical processor in frames of the astrophysical instrumentation is shortly discussed.

  13. River pollution remediation monitored by optical and infrared high-resolution satellite images.

    PubMed

    Trivero, Paolo; Borasi, Maria; Biamino, Walter; Cavagnero, Marco; Rinaudo, Caterina; Bonansea, Matias; Lanfri, Sofia

    2013-09-01

    The Bormida River Basin, located in the northwestern region of Italy, has been strongly contaminated by the ACNA chemical factory. This factory was in operation from 1892 to 1998, and contamination from the factory has had deleterious consequences on the water quality, agriculture, natural ecosystems and human health. Attempts have been made to remediate the site. The aims of this study were to use high-resolution satellite images combined with a classical remote sensing methodology to monitor vegetation conditions along the Bormida River, both upstream and downstream of the ACNA chemical factory site, and to compare the results obtained at different times before and after the remediation process. The trends of the Normalised Difference Vegetation Index (NDVI) and the Enhanced Vegetation Index (EVI) along the riverbanks are used to assess the effect of water pollution on vegetation. NDVI and EVI values show that the contamination produced by the ACNA factory had less severe effects in the year 2007, when most of the remediation activities were concluded, than in 2006 and 2003. In 2007, the contamination effects were noticeable up to 6 km downstream of the factory, whereas in 2003 and 2006 the influence range was up to about 12 km downstream of the factory. The results of this study show the effectiveness of remediation activities that have been taking place in this area. In addition, the comparison between NDVI and EVI shows that the EVI is more suitable to characterise the vegetation health and can be considered an additional tool to assess vegetation health and to monitor restoration activities. PMID:23456221

  14. A High-Resolution Oxygen Isotope Record for the Cariaco Basin, Venezuela Over the Last 6000 Years

    NASA Astrophysics Data System (ADS)

    Tedesco, K.; Thunell, R.; Tappa, E.

    2001-12-01

    Oxygen isotope records of three species of planktonic foraminifera (Globigerinoides ruber (pink), Globigerina bulloides and Neogloboquadrina dutertrei) from the Cariaco Basin, Venezuela were used to construct a high-resolution climate record for the Caribbean region for the mid to late Holocene. Our results indicate decreases in sea surface temperatures and/or increases in salinity in the basin at least four times over the last 4000 years (at ~3800-3200, 2800-2500, 2200-2000, and after 650 cal. yrs. B.P.). These events are coincident with high stands of Lake Titicaca, Bolivia/Peru suggesting a similar forcing mechanism. Paleoclimate records from the circum-Caribbean indicate arid conditions commenced in this region between about 3600-3200 cal. yrs. B.P.. This corresponds to a decrease in the δ 18O of G. ruber (pink) and decreases in the δ 18O differences between G. bulloides - G. ruber (pink) and N. dutertrei - G. ruber (pink). In addition, the deep dwelling planktonic foraminifera species Globorotalia crassaformis migrates into Cariaco Basin at about 3500 cal. yrs. B.P.. Taken together these data indicate colder sea surface temperatures and a shallow thermocline, possibly due to increased upwelling. Increased evaporation at this site would also result in higher G. ruber (pink) δ 18O values. Dry conditions in the circum-Caribbean tropics and wetter conditions in the Altiplano of Bolivia and Peru and the Amazon basin during the mid to late Holocene are consistent with a southward displacement of the Inter-Tropical Convergence Zone (ITCZ) resulting in decreased precipitation and increased trade wind intensity in the Cariaco Basin.

  15. A High-Resolution Record of Early Miocene Antarctic Glacial History From Downhole Logs, Site 1165, ODP Leg 188

    NASA Astrophysics Data System (ADS)

    Williams, T.; Barr, S.; Handwerger, D.

    2002-12-01

    ODP Site 1165, located 400 km NW of Prydz Bay, contains the most detailed record yet obtained of Early Miocene Antarctic glacial history. The 520-m-thick Early Miocene consists of dark grey claystone with silt laminae (contourites) alternating with decimeter-scale layers of greenish-grey bioturbated claystone that contain common ice rafted debris (IRD). These sediments are interpreted to record pulses of IRD from the continent, and alternations in the position or strength of the currents at the site. Downhole logs of electrical resistivity, natural gamma radiation, and density also record the alternations between the two facies. Here we assess the lithological significance of the logs by examining the opal, IRD, and susceptibility of two short intervals at high resolution, with samples taken every 10 cm, or about 1250 yr. The IRD-bearing greenish-grey claystone corresponds to higher resistivity and density because of increased cementation by silica, and corresponds to lower natural gamma radiation because of decreased clay content. Thus, using the logs, lithology can be inferred for intervals where no core was recovered. Based mainly on magnetostratigraphy, the age of the base of the hole is 22 Ma, and the sedimentation rate for the Early Miocene about 9 cm/kyr. The regular spacing of the facies alternations in the logs suggests they are astronomically paced. Between 18-20 Ma, the main periodicity is in the precessional range, and between 20-22 Ma, the logs have a saw-toothed form, with a major peak in IRD at the beginning of each, repeating about every 100 kyr. We think that the IRD pulses represent deglaciations. Hence our data suggest that the Antarctic continent, at least around the Prydz Bay region, was glaciated in the Early Miocene, and that the size of the ice sheet oscillated on Milankovitch time scales.

  16. Ultra-High Resolution Optical Coherence Tomography Imaging of Unilateral Drusen in a 31 Year Old Woman

    PubMed Central

    de Carlo, Talisa E; Adhi, Mehreen; Lu, Chen D; Duker, Jay S; Fujimoto, James G; Waheed, Nadia K

    2016-01-01

    We report a case of widespread unilateral drusen in a healthy 31 year old Caucasian woman using multi-modal imaging including ultra-high resolution optical coherence tomography (UHR-OCT). Dilated fundus exam showed multiple drusen-like lesions in the posterior pole without heme or fluid. Fundus auto fluorescence demonstrated hyperautofluorescent at the deposits. Fluorescein angiography revealed mild hyperfluorescence and staining of the lesions. Spectral-domain optical coherence tomography (SD-OCT) OS showed accumulations in the temporal macula at Bruch’s membrane. UHR-OCT provided improved axial resolution compared to the standard 5 μm on the commercial SD-OCT and confirmed the presence of deposits in Bruch’s membrane, consistent with drusen. The retinal layers were draped over the excrescences but did not show any disruption. PMID:27398405

  17. Investigation of solar active regions at high resolution by balloon flights of the solar optical universal polarimeter, definition phase

    NASA Technical Reports Server (NTRS)

    Tarbell, Theodore D.; Topka, Kenneth P.

    1992-01-01

    The definition phase of a scientific study of active regions on the sun by balloon flight of a former Spacelab instrument, the Solar Optical Universal Polarimeter (SOUP) is described. SOUP is an optical telescope with image stabilization, tunable filter and various cameras. After the flight phase of the program was cancelled due to budgetary problems, scientific and engineering studies relevant to future balloon experiments of this type were completed. High resolution observations of the sun were obtained using SOUP components at the Swedish Solar Observatory in the Canary Islands. These were analyzed and published in studies of solar magnetic fields and active regions. In addition, testing of low-voltage piezoelectric transducers was performed, which showed they were appropriate for use in image stabilization on a balloon.

  18. A scanning, all-fiber Sagnac interferometer for high resolution magneto-optic measurements at 820 nm

    SciTech Connect

    Fried, Alexander; Fejer, Martin; Kapitulnik, Aharon

    2014-10-15

    The Sagnac Interferometer has historically been used for detecting non-reciprocal phenomena, such as rotation. We demonstrate an apparatus in which this technique is employed for high resolution measurements of the Magneto-Optical Polar Kerr effect—a direct indicator of magnetism. Previous designs have incorporated free-space components which are bulky and difficult to align. We improve upon this technique by using all fiber-optic coupled components and demonstrate operation at a new wavelength, 820 nm, with which we can achieve better than 1 μrad resolution. Mounting the system on a piezo-electric scanner allows us to acquire diffraction limited images with 1.5 μm spatial resolution. We also provide extensive discussion on the details and of the Sagnac Interferometer's construction.

  19. Design and assessment of a wide FOV and high-resolution optical tiled head-mounted display.

    PubMed

    Song, Weitao; Cheng, Dewen; Deng, Zhaoyang; Liu, Yue; Wang, Yongtian

    2015-10-01

    It has always been a challenge to break the resolution/field-of-view (FOV) invariant to design a large FOV and high-resolution optical system, especially for a head-mounted display (HMD) system. In this study, a tiled HMD using two compact rotationally symmetrical eyepieces was designed and developed. Some issues on exit pupil and eye relief were analyzed in detail and taken into consideration during the design procedure. The overall optical system is compact with high performance. The system volume is smaller than 30  mm×35  mm×30  mm. Based on two 0.61 in. microdisplay devices, the overall tiled system demonstrates an FOV of 66°(H)×32°(V) with a 7.5 mm exit pupil diameter and a 15.7 mm eye relief. PMID:26479645

  20. Three-Dimensional High-Resolution Optical/X-Ray Stereoscopic Tracking Velocimetry

    NASA Technical Reports Server (NTRS)

    Cha, Soyoung S.; Ramachandran, Narayanan

    2004-01-01

    Measurement of three-dimensional (3-D) three-component velocity fields is of great importance in a variety of research and industrial applications for understanding materials processing, fluid physics, and strain/displacement measurements. The 3-D experiments in these fields most likely inhibit the use of conventional techniques, which are based only on planar and optically-transparent-field observation. Here, we briefly review the current status of 3-D diagnostics for motion/velocity detection, for both optical and x-ray systems. As an initial step for providing 3-D capabilities, we nave developed stereoscopic tracking velocimetry (STV) to measure 3-D flow/deformation through optical observation. The STV is advantageous in system simplicity, for continually observing 3- D phenomena in near real-time. In an effort to enhance the data processing through automation and to avoid the confusion in tracking numerous markers or particles, artificial neural networks are employed to incorporate human intelligence. Our initial optical investigations have proven the STV to be a very viable candidate for reliably measuring 3-D flow motions. With previous activities are focused on improving the processing efficiency, overall accuracy, and automation based on the optical system, the current efforts is directed to the concurrent expansion to the x-ray system for broader experimental applications.

  1. Three-Dimensional High-Resolution Optical/X-Ray Stereoscopic Tracking Velocimetry

    NASA Technical Reports Server (NTRS)

    Cha, Soyoung S.; Ramachandran, Naryanan

    2005-01-01

    Measurement of three-dimensional (3-D) three-component velocity fields is of great importance in a variety of research and industrial applications for understanding materials processing, fluid physics, and strain/displacement measurements. The 3-D experiments in these fields most likely inhibit the use of conventional techniques, which are based only on planar and optically-transparent-field observation. Here, we briefly review the current status of 3-D diagnostics for motion/velocity detection, for both optical and x-ray systems. As an initial step for providing 3-D capabilities, we have developed stereoscopic tracking velocimetry (STV) to measure 3-D flow/deformation through optical observation. The STV is advantageous in system simplicity, for continually observing 3-D phenomena in near real-time. In an effort to enhance the data processing through automation and to avoid the confusion in tracking numerous markers or particles, artificial neural networks are employed to incorporate human intelligence. Our initial optical investigations have proven the STV to be a very viable candidate for reliably measuring 3-D flow motions. With previous activities focused on improving the processing efficiency, overall accuracy, and automation based on the optical system, the current efforts is directed to the concurrent expansion to the x-ray system for broader experimental applications.

  2. X-ray optics. II - A technique for high resolution spectroscopy

    NASA Technical Reports Server (NTRS)

    Cash, Webster C., Jr.

    1991-01-01

    A novel combination of optical elements and properties is combined to achieve high-spectral resolution using grazing incidence optics of modest quality. Analysis and ray tracing of examples show that using radial groove gratings at high blaze angles in the manner of an echelle spectrograph can provide high spectral resolution. This arrangement is compared to the conventional in-plane designs to show that the off-plane is superior in nearly every respect. Cross dispersion can be provided by the energy resolution of a CCD detector. Additional resolution can be squeezed from the system by strategic placement of gratings to take advantage of the azimuthal response of a Wolter X-ray optic.

  3. Broadband miniature optical ultrasound probe for high resolution vascular tissue imaging.

    PubMed

    Colchester, Richard J; Zhang, Edward Z; Mosse, Charles A; Beard, Paul C; Papakonstantinou, Ioannis; Desjardins, Adrien E

    2015-04-01

    An all-optical ultrasound probe for vascular tissue imaging was developed. Ultrasound was generated by pulsed laser illumination of a functionalized carbon nanotube composite coating on the end face of an optical fiber. Ultrasound was detected with a Fabry-Pérot (FP) cavity on the end face of an adjacent optical fiber. The probe diameter was < 0.84 mm and had an ultrasound bandwidth of ~20 MHz. The probe was translated across the tissue sample to create a virtual linear array of ultrasound transmit/receive elements. At a depth of 3.5 mm, the axial resolution was 64 µm and the lateral resolution was 88 µm, as measured with a carbon fiber target. Vascular tissues from swine were imaged ex vivo and good correspondence to histology was observed. PMID:25909031

  4. High-Resolution Optical and Near-Infrared Imaging of Young Circumstellar Disks

    NASA Technical Reports Server (NTRS)

    McCaughrean, Mark; Stapelfeldt, Karl; Close, Laird

    2000-01-01

    In the past five years, observations at optical and near-infrared wavelengths obtained with the Hubble Space Telescope and ground-based adaptive optics have provided the first well-resolved images of young circumstellar disks which may form planetary systems. We review these two observational techniques and highlight their results by presenting prototype examples of disks imaged in the Taurus-Auriga and Orion star-forming regions. As appropriate, we discuss the disk parameters that may be typically derived from the observations, as well as the implications that the observations may have on our understanding of, for example, the role of the ambient environment in shaping the disk evolution. We end with a brief summary of the prospects for future improvements in space- and ground-based optical/IR imaging techniques, and how they may impact disk studies.

  5. Broadband miniature optical ultrasound probe for high resolution vascular tissue imaging

    PubMed Central

    Colchester, Richard J.; Zhang, Edward Z.; Mosse, Charles A.; Beard, Paul C.; Papakonstantinou, Ioannis; Desjardins, Adrien E.

    2015-01-01

    An all-optical ultrasound probe for vascular tissue imaging was developed. Ultrasound was generated by pulsed laser illumination of a functionalized carbon nanotube composite coating on the end face of an optical fiber. Ultrasound was detected with a Fabry-Pérot (FP) cavity on the end face of an adjacent optical fiber. The probe diameter was < 0.84 mm and had an ultrasound bandwidth of ~20 MHz. The probe was translated across the tissue sample to create a virtual linear array of ultrasound transmit/receive elements. At a depth of 3.5 mm, the axial resolution was 64 µm and the lateral resolution was 88 µm, as measured with a carbon fiber target. Vascular tissues from swine were imaged ex vivo and good correspondence to histology was observed. PMID:25909031

  6. Use of tapered amplifier diode laser for biological-friendly high-resolution optical trapping.

    PubMed

    Cheng, Wei; Hou, Ximiao; Ye, Fangmao

    2010-09-01

    A 1064 nm laser is commonly used for biological optical trapping. However, it has the problem of generating reactive oxygen species in the presence of a sensitizer, which leads to photo damage in biological samples. Here we constructed optical tweezers using a tapered amplifier diode laser that operates at 830 nm. Compared to a 1064 nm laser, this laser is friendly to live cells, eliminates photo damage associated with reactive oxygen species, and allows simultaneous two-photon fluorescence imaging of green fluorescent proteins in live mammalian cells. All these advantages could significantly benefit future application of this single molecule technique in biological studies. PMID:20808392

  7. Optical sedimentation recorder

    DOEpatents

    Bishop, James K.B.

    2014-05-06

    A robotic optical sedimentation recorder is described for the recordation of carbon flux in the oceans wherein both POC and PIC particles are captured at the open end of a submersible sampling platform, the captured particles allowed to drift down onto a collection plate where they can be imaged over time. The particles are imaged using three separate light sources, activated in sequence, one source being a back light, a second source being a side light to provide dark field illumination, and a third source comprising a cross polarized light source to illuminate birefringent particles. The recorder in one embodiment is attached to a buoyancy unit which is capable upon command for bringing the sedimentation recorder to a programmed depth below the ocean surface during recordation mode, and on command returning the unit to the ocean surface for transmission of recorded data and receipt of new instructions. The combined unit is provided with its own power source and is designed to operate autonomously in the ocean for extended periods of time.

  8. Carbon cycle dynamics and solar activity embedded in a high-resolution 14C speleothem record from Belize, Central America

    NASA Astrophysics Data System (ADS)

    Lechleitner, Franziska A.; Breitenbach, Sebastian F. M.; McIntyre, Cameron; Asmerom, Yemane; Prufer, Keith M.; Polyak, Victor; Culleton, Brendan J.; Kennett, Douglas J.; Eglinton, Timothy I.; Baldini, James U. L.

    2015-04-01

    Speleothem 14C has recently emerged as a potentially powerful proxy for climate reconstruction. Several studies have highlighted the link between karst hydrology and speleothem 14C content, and a number of possible causes for this relationship have been proposed, such as dripwater flow dynamics in the karst and changes in soil organic matter (SOM) turnover time (e.g. Griffiths et al., 2012). Here we present a high resolution 14C record for a stalagmite (YOK-I) from Yok Balum cave in southern Belize, Central America. YOK-I grew continuously over the last 2000 years, and has been dated very precisely with the U-Th method (40 dates, mean uncertainty < 10 years). The excellent chronological control for this stalagmite allows us to calculate 14C activity (a14C) at the time of speleothem deposition (a14Cinit), as well as the dead carbon fraction (DCF), predominantly a measure of the reservoir effect introduced by limestone dissolution in the karst (Genty et al., 2001). Both records show striking similarities to atmospheric a14C (IntCal13) and reconstructions of solar activity and 14C production rate. We infer close coupling between cave environment and atmosphere, with minimal signal dampening, an observation supported by monitoring data (Ridley et al., in press). DCF fluctuates between approximately 10% and 16% over the entire record, with distinctly lower DCF values and higher a14Cinit during a period of reduced rainfall between ca. 700-1100 AD (linked to the Classic Maya Collapse). This behavior is consistent with observations made elsewhere, and suggests that DCF responds to karst hydrological variability, specifically open-closed system transitions. YOK-I a14Cinit typically lags atmospheric values by 10-100 cal years. A shorter lag appears to be linked to periods of drought, suggesting a response of SOM dynamics above the cave to rainfall reduction. Specifically, drought is inferred to lead to reduced bioproductivity and soil carbon turnover, lowering contributions

  9. Fiber-Optic Strain Gauge With High Resolution And Update Rate

    NASA Technical Reports Server (NTRS)

    Figueroa, Fernando; Mahajan, Ajay; Sayeh, Mohammad; Regez, Bradley

    2007-01-01

    An improved fiber-optic strain gauge is capable of measuring strains in the approximate range of 0 to 50 microstrains with a resolution of 0.1 microstrain. (To some extent, the resolution of the strain gauge can be tailored and may be extensible to 0.01 microstrain.) The total cost of the hardware components of this strain gauge is less than $100 at 2006 prices. In comparison with prior strain gauges capable of measurement of such low strains, this strain gauge is more accurate, more economical, and more robust, and it operates at a higher update rate. Strain gauges like this one are useful mainly for measuring small strains (including those associated with vibrations) in such structures as rocket test stands, buildings, oilrigs, bridges, and dams. The technology was inspired by the need to measure very small strains on structures supporting liquid oxygen tanks, as a way to measure accurately mass of liquid oxygen during rocket engine testing. This improved fiber-optic strain gauge was developed to overcome some of the deficiencies of both traditional foil strain gauges and prior fiber-optic strain gauges. Traditional foil strain gages do not have adequate signal-to-noise ratios at such small strains. Fiber-optic strain gauges have been shown to be potentially useful for measuring such small strains, but heretofore, the use of fiberoptic strain gauges has been inhibited, variously, by complexity, cost, or low update rate.

  10. High Resolution Optical/Near-Infrared Imaging of Cool Ultraluminous Infrared Galaxies

    NASA Technical Reports Server (NTRS)

    Surace, J.; Sanders, D.; Evans, A.

    1999-01-01

    We present here new multiwavelength observations with 1.5 and 4x the spatial resolution of previous ground-based observations at optical and near-infrared wavelengths; despite being ground-based, they allow us to isolate interesting features such as the star-forming knots detected in the warm ULIG sample.