Sample records for holothuria scabra collected

  1. Maturity Gonad Sea Cucumber Holothuria scabra Under The Month Cycle

    NASA Astrophysics Data System (ADS)

    Penina Tua Rahantoknam, Santi

    2017-10-01

    Gonad maturity level of the sea cucumber Holothuria scabra is important to note for selection of parent ready spawn. Sea cucumbers are giving a reaction to the treatment of excitatory spawn mature individuals only. For the determination of the level of maturity of gonads of sea cucumbers, the necessary observation of the gonads are microscopic, macroscopic and gonad maturity gonado somatic indeks (GSI). GSI value is important to know the changes that occur in the gonads quantitatively, so that time can be presumed spawning (Effendie, 1997). Reproductive cycle can be determined by observing the evolution of GSI. The study of sea cucumbers Holothuria scabra gonad maturity conducted in Langgur, Southeast Maluku. Observations were made at every cycle of the moon is the full moon phase (BP) and new moon (BB) in the period January 29, 2017 until July 23, 2017. Observations H. scabra gonad maturity level is done with surgery, observation and calculation GSI gonad histology. GSI highest value obtained in May that full moon cycle at 90% of individuals that are in the spawning stage (phase 5), then 70% of the individuals that are in the spawning stage (phase 5) in March that the full moon cycle. The results obtained show that the peak spawning H. scabra period January 2017 to July 2017 occurred on the full moon cycle in May.

  2. Effects of sandfish (Holothuria scabra) removal on shallow-water sediments in Fiji.

    PubMed

    Lee, Steven; Ford, Amanda K; Mangubhai, Sangeeta; Wild, Christian; Ferse, Sebastian C A

    2018-01-01

    Sea cucumbers play an important role in the recycling and remineralization of organic matter (OM) in reef sands through feeding, excretion, and bioturbation processes. Growing demand from Asian markets has driven the overexploitation of these animals globally. The implications of sea cucumber fisheries for shallow coastal ecosystems and their management remain poorly understood. To address this knowledge gap, the current study manipulated densities of Holothuria scabra within enclosures on a reef flat in Fiji, between August 2015 and February 2016, to study the effects of sea cucumber removal on sedimentary function as a biocatalytic filter system. Three treatments were investigated: (i) high density (350 g m -2 wet weight; ca . 15 individuals); (ii) natural density (60 g m -2 ; ca . 3 individuals); and (iii) exclusion (0 g m -2 ). Quantity of sediment reworked through ingestion by H. scabra , grain size distribution, O 2 penetration depth, and sedimentary oxygen consumption (SOC) were quantified within each treatment. Findings revealed that the natural population of H. scabra at the study site can rework ca . 10,590 kg dry sediment 1,000 m -2 year -1 ; more than twice the turnover rate recorded for H. atra and Stichopus chloronotus . There was a shift towards finer fraction grains in the high treatment. In the exclusion treatment, the O 2 penetration depth decreased by 63% following a 6 °C increase in water temperature over the course of two months, while in the high treatment no such change was observed. SOC rates increased ca . two-fold in the exclusion treatment within the first month, and were consistently higher than in the high treatment. These results suggest that the removal of sea cucumbers can reduce the capacity of sediments to buffer OM pulses, impeding the function and productivity of shallow coastal ecosystems.

  3. Effects of sandfish (Holothuria scabra) removal on shallow-water sediments in Fiji

    PubMed Central

    Ford, Amanda K.; Mangubhai, Sangeeta; Wild, Christian; Ferse, Sebastian C.A.

    2018-01-01

    Sea cucumbers play an important role in the recycling and remineralization of organic matter (OM) in reef sands through feeding, excretion, and bioturbation processes. Growing demand from Asian markets has driven the overexploitation of these animals globally. The implications of sea cucumber fisheries for shallow coastal ecosystems and their management remain poorly understood. To address this knowledge gap, the current study manipulated densities of Holothuria scabra within enclosures on a reef flat in Fiji, between August 2015 and February 2016, to study the effects of sea cucumber removal on sedimentary function as a biocatalytic filter system. Three treatments were investigated: (i) high density (350 g m−2 wet weight; ca. 15 individuals); (ii) natural density (60 g m−2; ca. 3 individuals); and (iii) exclusion (0 g m−2). Quantity of sediment reworked through ingestion by H. scabra, grain size distribution, O2 penetration depth, and sedimentary oxygen consumption (SOC) were quantified within each treatment. Findings revealed that the natural population of H. scabra at the study site can rework ca. 10,590 kg dry sediment 1,000 m−2 year−1; more than twice the turnover rate recorded for H. atra and Stichopus chloronotus. There was a shift towards finer fraction grains in the high treatment. In the exclusion treatment, the O2 penetration depth decreased by 63% following a 6 °C increase in water temperature over the course of two months, while in the high treatment no such change was observed. SOC rates increased ca. two-fold in the exclusion treatment within the first month, and were consistently higher than in the high treatment. These results suggest that the removal of sea cucumbers can reduce the capacity of sediments to buffer OM pulses, impeding the function and productivity of shallow coastal ecosystems. PMID:29796344

  4. Evidence for a Saponin Biosynthesis Pathway in the Body Wall of the Commercially Significant Sea Cucumber Holothuria scabra.

    PubMed

    Mitu, Shahida Akter; Bose, Utpal; Suwansa-Ard, Saowaros; Turner, Luke H; Zhao, Min; Elizur, Abigail; Ogbourne, Steven M; Shaw, Paul Nicholas; Cummins, Scott F

    2017-11-07

    The sea cucumber (phylum Echinodermata) body wall is the first line of defense and is well known for its production of secondary metabolites; including vitamins and triterpenoid glycoside saponins that have important ecological functions and potential benefits to human health. The genes involved in the various biosynthetic pathways are unknown. To gain insight into these pathways in an echinoderm, we performed a comparative transcriptome analysis and functional annotation of the body wall and the radial nerve of the sea cucumber Holothuria scabra ; to define genes associated with body wall metabolic functioning and secondary metabolite biosynthesis. We show that genes related to signal transduction mechanisms were more highly represented in the H. scabra body wall, including genes encoding enzymes involved in energy production. Eight of the core triterpenoid biosynthesis enzymes were found, however, the identity of the saponin specific biosynthetic pathway enzymes remains unknown. We confirm the body wall release of at least three different triterpenoid saponins using solid phase extraction followed by ultra-high-pressure liquid chromatography-quadrupole time of flight-mass spectrometry. The resource we have established will help to guide future research to explore secondary metabolite biosynthesis in the sea cucumber.

  5. The timing and reproductive output of the commercial sea cucumber Holothuria scabra on the Kenyan coast

    NASA Astrophysics Data System (ADS)

    Muthiga, N. A.; Kawaka, J. A.; Ndirangu, S.

    2009-09-01

    The sea cucumber Holothuria scabra is a widely distributed and economically important species that has been harvested in Kenya for decades. No previous studies have been carried out on the reproduction of this species in Kenya. Standard gonad index methods were used to analyze reproductive patterns of individuals collected monthly in 1998-1999, 2000-2001 and 2006-2007. Morphological characteristics, gonad tubule lengths and fecundity were also measured. Mean monthly gonad indices were significantly correlated between males and females indicating synchronous gonad development between the sexes. Gonad indices showed a biannual pattern that was consistent in all three years with a minor spawning event occurring between August and September and a major spawning event between November and December. The pattern of gonad growth showed significant variability between years and between months. Temporal changes in gonad growth correlated significantly with gonad tubule length and absolute fecundity. Monthly gonad indices also correlated significantly with monthly measurements of air temperature and light suggesting a possible role for both factors in timing gametogenesis and spawning. There was a shift in sex ratio from unity in the 1998-1999 and 2000-2001 samples to significantly more males in the 2006-2007 samples, as well as a significant reduction in mean sizes (body wall weight) and reproductive output (gonad index) which suggests that the reproductive success of this species is potentially negatively affected by fishing.

  6. Acid-base physiology response to ocean acidification of two ecologically and economically important holothuroids from contrasting habitats, Holothuria scabra and Holothuria parva.

    PubMed

    Collard, Marie; Eeckhaut, Igor; Dehairs, Frank; Dubois, Philippe

    2014-12-01

    Sea cucumbers are dominant invertebrates in several ecosystems such as coral reefs, seagrass meadows and mangroves. As bioturbators, they have an important ecological role in making available calcium carbonate and nutrients to the rest of the community. However, due to their commercial value, they face overexploitation in the natural environment. On top of that, occurring ocean acidification could impact these organisms, considered sensitive as echinoderms are osmoconformers, high-magnesium calcite producers and have a low metabolism. As a first investigation of the impact of ocean acidification on sea cucumbers, we tested the impact of short-term (6 to 12 days) exposure to ocean acidification (seawater pH 7.7 and 7.4) on two sea cucumbers collected in SW Madagascar, Holothuria scabra, a high commercial value species living in the seagrass meadows, and H. parva, inhabiting the mangroves. The former lives in a habitat with moderate fluctuations of seawater chemistry (driven by day-night differences) while the second lives in a highly variable intertidal environment. In both species, pH of the coelomic fluid was significantly negatively affected by reduced seawater pH, with a pronounced extracellular acidosis in individuals maintained at pH 7.7 and 7.4. This acidosis was due to an increased dissolved inorganic carbon content and pCO2 of the coelomic fluid, indicating a limited diffusion of the CO2 towards the external medium. However, respiration and ammonium excretion rates were not affected. No evidence of accumulation of bicarbonate was observed to buffer the coelomic fluid pH. If this acidosis stays uncompensated for when facing long-term exposure, other processes could be affected in both species, eventually leading to impacts on their ecological role.

  7. Destruction of a Holothuria scabra population by overfishing at Abu Rhamada Island in the Red Sea.

    PubMed

    Hasan, Mohamed Hamza

    2005-10-01

    Populations of Holothuria scabra at Abu Rhamada Island were investigated during 52 months, from July 1999 to October 2003. During the first 23 months (July, 1999-May, 2001) the Island had a robust population with a tri-modal size frequency distribution curve, very high densities (85.7-95.1 ind./100 m2 at the sandy habitat), high abundance (3362-3110 individuals) and biomass (46.7-34.3 kg/100 m2). Also, during this period most individuals were at depths between 4 and 6m and no individuals were recorded deeper than 15m. The population declined after harvesting began (June, 2001) and by March, 2002 the size frequency distribution showed a bimodal pattern with an obvious decrease in abundance of large individuals. There was also a slight reduction in densities (73.2-60.1 ind./100 m2 at the sandy habitat), abundance (2292-1682 individuals) and biomass (21.6-11.3 kg/100 m2), and a marked shift towards deeper waters. Overfishing reached its maximum during the final 19 months of the study, and by October, 2003, density (30.7-0.4 ind./100 m2 at the sandy habitat), abundance (802-10 individuals) and biomass (6.9-0.1 kg/100 m2) were all greatly reduced. The size frequency distribution of the population became unimodal, large animals disappeared and no recruits were seen. During this period, individuals were found at very deep depths (30 to >40 m). The study also showed that sandy substrate was the preferred habitat for H. scabra, accommodating the largest number of individuals. The population of H. scabra at Abu Rhamada Island was found to spawn biannually from 1999 to 2001, then only once during 2002 when high fishing pressure occurred, and ceased completely in 2003. The sex ratio was not significantly different from 1:1 before fishing begun, but shifted to an increasing male bias reaching 93% males by January 2003. None of the small animals remaining after January, 2003 could be sexed. Size at sexual maturity decreased from prefishing (185 mm for females and 160 mm for

  8. Development of novel microsatellite markers for Holothurian scabra (Holothuriidae), Apostichopus japonicas (Stichopodidae) and cross-species testing in other sea cucumbers

    NASA Astrophysics Data System (ADS)

    Shangguan, Jingbo; Li, Zhongbao

    2017-06-01

    Thirty-five new microsatellite loci from the sea cucumbers Holothurian scabra (Jaeger, 1833) and Apostichopus japonicas (Selenka, 1867) were screened and characterized using the method of magnetic bead enrichment. Of the twenty-four polymorphic loci tested, eighteen were consistent with Hardy-Weinberg equilibrium after a modified false discovery rate (B-Y FDR) correction, whereas six showed statistically significant deviations (CHS2 and CHS11: P <0.014790; FCS1, FCS6, FCS8 and FCS14: P <0.015377). Furthermore, four species of plesiomorphous and related sea cucumbers (Holothurian scabra, Holothuria leucospilota, Stichopus horrens and Apostichopus japonicas) were tested for mutual cross-amplification using a total of ninety microsatellite loci. Although transferability and universality of all loci were generally low, the results of the cross-species study showed that the markers can be applied to identify individuals to species according to the presence or absence of specific microsatellite alleles. The microsatellite markers reported here will contribute to the study of genetic diversity, assisted breeding, and population conservation in sea cucumbers, as well as allow for the identification of individuals to closely related species.

  9. Development of novel microsatellite markers for Holothurian scabra (Holothuriidae), Apostichopus japonicas (Stichopodidae) and cross-species testing in other sea cucumbers

    NASA Astrophysics Data System (ADS)

    Shangguan, Jingbo; Li, Zhongbao

    2018-03-01

    Thirty-five new microsatellite loci from the sea cucumbers Holothurian scabra (Jaeger, 1833) and Apostichopus japonicas (Selenka, 1867) were screened and characterized using the method of magnetic bead enrichment. Of the twenty-four polymorphic loci tested, eighteen were consistent with Hardy-Weinberg equilibrium after a modified false discovery rate (B-Y FDR) correction, whereas six showed statistically significant deviations (CHS2 and CHS11: P<0.014 790; FCS1, FCS6, FCS8 and FCS14: P<0.015 377). Furthermore, four species of plesiomorphous and related sea cucumbers ( Holothurian scabra, Holothuria leucospilota, Stichopus horrens and Apostichopus japonicas) were tested for mutual cross-amplification using a total of ninety microsatellite loci. Although transferability and universality of all loci were generally low, the results of the cross-species study showed that the markers can be applied to identify individuals to species according to the presence or absence of specific microsatellite alleles. The microsatellite markers reported here will contribute to the study of genetic diversity, assisted breeding, and population conservation in sea cucumbers, as well as allow for the identification of individuals to closely related species.

  10. [Studies on extraction process optimization of patrinia scabra Bunge saponins].

    PubMed

    Wang, Xue-Xi; Chen, Ru; Li, Shi-Gang; Shen, Wei; Cheng, Wei-Dong; Zhao, Jian-Xiong

    2007-05-01

    To optimize the conditions for the extraction of Patrinia scabra Bunge saponins. Orthogonal experimental design and ultrasonic method were employed to examine the conditions for the extraction by determination of saponins. The optimun condition for the extraction of Patrinia scabra Bunge saponins was as follows: 65% ethanol for 40 minutes, 55 degrees C and 210 watt of ultrasonic efficinecy. The extraction method of Patrinia scabra Bunge sponins is simple and efficient.

  11. In vitro propagation of Gentiana scabra Bunge - an important medicinal plant in the Chinese system of medicines.

    PubMed

    Huang, Shih-Hung; Agrawal, Dinesh Chandra; Wu, Fang-Sheng; Tsay, Hsin-Sheng

    2014-12-01

    Gentiana scabra Bunge commonly known as 'Long dan cao' in China has been used in traditional Chinese medicines for more than 2000 years. Dry roots and rhizome of the herb have been used for the treatment of inflammation, anorexia, indigestion and gastric infections. Iridoids and secoiridoids are the main bioactive compounds which attribute to the pharmacological properties of this plant. The species is difficult to mass propagate by seed due to the low percentage of germination and limited dormancy period. Wild populations in some locations are considered to be in the endangered category due to over exploitation. In the present study, we report an efficient micropropagation system. Shoot apices of six weeks old in vitro grown G. scabra plants were used as explants for the in vitro propagation. Induction of multiple shoots (9.1/explant) was achieved on the culture of shoot apices on half strength Murashige and Skoog's basal medium (MSBM) containing 2.0 mg/L -1 6-benzylaminopurine (BA), 3% sucrose and 0.9% Difco agar. In vitro shoots induced profuse rooting on half strength of MSBM supplemented with 0.1 mg/L -1 1-naphthaleneacetic acid (NAA), 3% sucrose and 0.3% gelrite. A two-stage ventilation closure procedure during the in vitro culture, and transparent sachet technique enhanced the survival rate of G. scabra plantlets to 96% in the greenhouse. Tissue culture plants flowered after 5 months of transfer to pots. A simple and an efficient in vitro propagation protocol of Gentiana scabra Bunge by optimizing the medium composition and ventilation closure treatments has been developed. The protocol can be very useful in germplasm conservation and commercial cultivation of G. scabra plants.

  12. Effects of seven diets on the population dynamics of laboratory cultured Tisbe holothuriae Humes (Copepoda, Harpacticoida)

    NASA Astrophysics Data System (ADS)

    Miliou, H.; Moraïtou-Apostolopoulou, M.

    1991-09-01

    The harpacticoid copepod Tisbe holothuriae was collected from Saronicos Gulf (Greece) and reared under constant laboratory conditions. In order to study the effects of food on the population dynamics, seven diets were tested: the seaweed Ulva; five artificial compound feeds: the liquid Fryfood® (Waterlife), a powder of Mytilus, yeast, soya and Spirulina, respectively; and a mixed diet consisting of Ulva and Fryfood. The life cycle parameters (mortality, sex ratio, generation time, offspring production) were measured, and the demographic variables [mean generation time (T), net reproductive rate (Ro), and intrinsic rate of natural increase (rm)] were determined. As to their efficiency regarding population dynamics, the diets ranked as follows: (1) Ulva+Fryfood, (2), Ulva, (3) Fryfood, (4) Mytilus, (5) soya, (6) yeast, and (7) Spirulina. In this order they cause a progressive increase of both larval mortality and generation time, a progressive decrease of sex ratio, number of offspring per egg sac, number of egg sacs per female and, consequently, of Ro and rm. The observed differences between diets were most pronounced with respect to offspring production. Of the compound diets, those containing animal extracts were more efficient than those containing vegetable materials. Ulva plays an important role in the nutrition of T. holothuriae, favouring offspring production as well as larval survival, development and pigmentation. Ulva in combination with Fryfood led to a greater copepodid survival and offspring production. This mixed diet proved to be the most favourable for rearing the Greek population of t. holothuriae, resulting in an efficient intrinsic rate of natural increase (rm=0.304) of the population.

  13. Effects of processing on proximate and fatty acid compositions of six commercial sea cucumber species of Sri Lanka.

    PubMed

    Nishanthan, G; Kumara, P A D A; de Croos, M D S T; Prasada, D V P; Dissanayake, D C T

    2018-05-01

    Processing and its impacts on proximate composition and fatty acid profile of six sea cucumber species; Bohadschia marmorata, Stichopus chloronotus, Holothuria spinifera, Thelenota anax, Holothuria scabra and Bohadschia sp. 1 collected from the northwest coast of Sri Lanka were analyzed. Sea cucumbers are processed into bêche - de - mer by both domestic and industrial level processors following the similar steps of cleaning, evisceration, first boiling, salting, second boiling and drying. However, domestically processed bêche - de - mer always reported a higher percentage of moisture, crude ash, crude fat and lower percentage of crude protein than industrially processed products. Although processing resulted in a significant reduction of total SFA and MUFA in fresh individuals of most of these species, total PUFA increased significantly in processed individuals excluding Bohadschia species. Palmitic acid was found to be the most dominant fatty acid in all these species followed by eicosapentaenoic acid, which showed a significant increase in processed products, except Bohadschia sp. 1. Total MUFA were higher than total SFA in all sea cucumber species having exceptions in Bohadchia sp.1 and fresh S. chloronotus. These findings will make a significant contribution to fill the gaps in existing information as no any previous information is available for species like H. spinifera and S. chloronotus .

  14. First report of powdery mildew on cucumis zambianus, cucurbita digitata and zehneria scabraCaused by podosphaera xanthii

    USDA-ARS?s Scientific Manuscript database

    Powdery mildew is a serious disease of cucurbit crops worldwide. In the fall of 2016, symptoms of powdery mildew were observed on 2-month old plants of Cucumis zambianus, Cucurbita digitata and Zehneria scabra in research plots in Charleston, SC. Incidence on 28 plants of C. zambianus was 64.3%. On ...

  15. Cytotoxic effect of Alpinia scabra (Blume) Náves extracts on human breast and ovarian cancer cells

    PubMed Central

    2013-01-01

    Background Alpinia scabra, locally known as 'Lengkuas raya’, is an aromatic, perennial and rhizomatous herb from the family Zingiberaceae. It is a wild species which grows largely on mountains at moderate elevations in Peninsular Malaysia, but it can also survive in the lowlands like in the states of Terengganu and Northern Johor. The present study reports the cytotoxic potential of A. scabra extracts from different parts of the plant. Methods The experimental approach in the present study was based on a bioassay-guided fractionation. The crude methanol and fractionated extracts (hexane, chloroform and water) from different parts of A. scabra (leaves, rhizomes, roots and pseudo stems) were prepared prior to the cytotoxicity evaluation against human ovarian (SKOV-3) and hormone-dependent breast (MCF7) carcinoma cells. The identified cytotoxic extracts were then subjected to chemical investigations in order to identify the active ingredients. A normal human lung fibroblast cell line (MRC-5) was used to determine the specificity for cancerous cells. The cytotoxic extracts and fractions were also subjected to morphological assessment, DNA fragmentation analysis and DAPI nuclear staining. Results The leaf (hexane and chloroform) and rhizome (chloroform) extracts showed high inhibitory effect against the tested cells. Ten fractions (LC1-LC10) were yielded after purification of the leaf chloroform extract. Fraction LC4 which showed excellent cytotoxic activity was further purified and resulted in 17 sub-fractions (VLC1-VLC17). Sub-fraction VLC9 showed excellent cytotoxicity against MCF7 and SKOV-3 cells but not toxic against normal MRC-5 cells. Meanwhile, eighteen fractions (RC1-RC18) were obtained after purification of the rhizome chloroform extract, of which fraction RC5 showed cytotoxicity against SKOV-3 cells with high selectivity index. There were marked morphological changes when observed using phase-contrast inverted microscope, DAPI nuclear staining and also DNA

  16. Anti-diabetic activity of Holothuria thomasi saponin.

    PubMed

    El Barky, Amira R; Hussein, Samy A; Alm-Eldeen, Abeer A; Hafez, Yehia A; Mohamed, Tarek M

    2016-12-01

    Diabetes mellitus represents a global health problem. It characterized by hyperglycemia that induces oxidative stress leading to a generation of free radicals. A wide variety of natural products in plants and other marine animals represent antioxidant activity and other health benefits like those of sea cucumber. Therefore, this study aimed to investigate the antidiabetic activity of glycosidic compound - saponin - derived from the Egyptian sea cucumber, Holothuria thomasi. Saponin has been extracted from the Egyptian sea cucumber and confirmed by hemolysis, Salkowski tests, FT/IR, UV and GC-MS analysis. Eighty white female albino rats were divided into four equal groups. The first two groups of rats; control normal and control normal saponin-treated groups. The last two groups which were made diabetic by intraperitoneal injection of streptozotocin had one diabetic control and the other diabetic group that got 300mg/kg B.wt. of saponin extract after Thirty-five days after diabetes induction and lasted for six weeks. The functional group of saponin extract which established with FT/IR spectroscopy demonstrated the presence of saponin in the extracted materials as shown in the peak of the functional group in relevance to the standard one. The UV spectra revealed that λ max of saponin extract was 282nm which in accordance to the standard saponin. Also, GC-MS analysis indicated that the aglycone part of saponin was methyl esters of octadecanoic acid. Saponin extract significantly decreased serum glucose, α-amylase activity, adiponectin, IL-6, TNF-α concentrations and liver L-MDA. However, serum insulin and liver glycogen levels were significantly increased as compared with the diabetic non-treated groups. The histopathological results supported that saponin extract markedly reduced the degenerative change in β-cells. This study, therefore, depicts that the Egyptian Holothuria thomasi, sea cucumber saponin as a hypoglycemic agent with the potential to normalize

  17. Screening of microbial contamination and antimicrobial activity of sea cucumber Holothuria polii.

    PubMed

    Omran, Nahla E E; Allam, Nanis G

    2013-11-01

    Microbiological studies were carried out on microbial contamination and antimicrobial activity of sea cucumber Holothuria polii collected from Mediterranean Sea at Abu-kir shore of Alexandria, Egypt. The obtained results revealed the presence of isolates of five human Gram-negative pathogenic bacteria, representing five genera were identified to species level, including, Esherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella sp. and Shigella sp. In addition, an yeast Candida albicans was isolated. The pathogenic bacteria were identified using API 20E strip system (BioMereux). All collected H. polii specimens were healthy with no external signs of infection. Histopathological study of the tegument, intestine and gonads showed no abnormal changes. The antimicrobial activity of two tegumental ethanol extracts (A and B, differ in the method of dehydration) were tested against wide range of pathogenic bacteria and fungi, including intestinal, skin and nosocomial pathogens and one plant fungal pathogen. The results revealed a remarkable antifungal activity of the extract B at 2.5 mg/ml MIC90, especially on Aspergillus niger, Scloretium sp, C. albicans, Aspergillus flavus and Malassezia furfur, and limited antibacterial activity against Gram-negative bacteria (Salmonella choleraesuis ATCC 14028 and Aeromonas hydrophila). The domain of bacterial and limited fungal contamination confirms the results that showed strong antifungal activity of investigated extract.

  18. Cytotoxic and anti-colorectal tumor effects of sulfated saponins from sea cucumber Holothuria moebii.

    PubMed

    Yu, Siran; Ye, Xuewei; Chen, Lu; Xie, Xin; Zhou, Qian; Lian, Xiao-Yuan; Zhang, Zhizhen

    2015-11-15

    Whether sulfated saponins from Holothuria moebii inhibit the proliferation of colorectal cancer cells and have anti-colorectal tumor effects in animal model has not been investigated. To evaluate the cytotoxic and anti-colorectal tumor effects of sulfated saponins from sea cucumber Holothuria moebii. (1) Column chromatography was used to prepare the total and individual saponins and HPLC was applied to define the components of the total saponins; (2) the activity of the total and individual saponins inhibiting the proliferation of human colorectal cancer cells was determined by SRB assay and the apoptosis induced by the saponins was qualified using cytometric analysis with Annexin V-FITC/PI double staining; and (3) the antitumor effects of the sulfated saponins on colorectal CT-26 tumor-bearing Balb/c mice were tested. The total and individual sulfated saponins significantly inhibited the proliferation of four different human colorectal cancer cells with IC50 values ranging from 1.04 to 4.08 μM (or 1.46 to 3.24 μg/ml for total saponins) and induced late apoptosis at an early treatment time in cancer cells. The total saponins (120 mg/kg) had antitumor activity in colorectal CT-26 tumor-bearing Balb/c mice. The sulfated saponins from H. moebii remarkably inhibited the proliferation of different human colorectal cancer cells and had significant anti-colorectal tumor activity in animal model. Copyright © 2015 Elsevier GmbH. All rights reserved.

  19. Antioxidant and immunological activities of polysaccharides from Gentiana scabra Bunge roots.

    PubMed

    Wang, Zhanyong; Wang, Chenyu; Su, Tingting; Zhang, Jing

    2014-11-04

    Two polysaccharide fractions, GSP I-a and GSP II-b, were isolated from Gentiana scabra Bunge roots. Both GSP I-a and GSP II-b comprised seven monosaccharides: fructose, mannose, rhamnose, galacturonic acid, glucose, galactose, and fucose. Ultraviolet and infrared analyses show that GSP I-a and GSP II-b are proteoglycans. In vitro evaluation of the antioxidant activity suggests that GSP I-a and GSP II-b scavenge 1,1-diphenyl-2-picrylhydrazyl radicals. However, the scavenging activity of the latter is stronger than that of the former. GSP I-a and GSP II-b have relatively low reducing powers and scavenging activities toward superoxide anions and hydroxyls. GSP I-a and GSP II-b significantly increase lymphocyte proliferation when lipopolysaccharide is used as a mitogen for lymphocytes, but only GSP I-a can significantly increase lymphocyte proliferation within the test-dosage range when concanavalin A is used as a mitogen. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. Multi-residue analysis of 36 priority and emerging pollutants in marine echinoderms (Holothuria tubulosa) and marine sediments by solid-liquid extraction followed by dispersive solid phase extraction and liquid chromatography-tandem mass spectrometry analysis.

    PubMed

    Martín, J; Zafra-Gómez, A; Hidalgo, F; Ibáñez-Yuste, A J; Alonso, E; Vilchez, J L

    2017-05-01

    Marine echinoderms are filter-feeding invertebrates widely distributed along the coasts, and which are therefore extensively exposed to anthropogenic xenobiotics. They can serve as good sentinels for monitoring a large variety of contaminants in marine ecosystems. In this context, a multi-residue analytical method has been validated and applied to Holothuria tubulosa specimens and marine sediments for the determination of 36 organic compounds, which belong to some of the most problematic groups of emerging and priority pollutants (perfluoroalkyl compounds, estrogens, parabens, benzophenones, plasticizers, surfactants, brominated flame retardants and alkylphenols). Lyophilization of samples prior to solvent extraction and clean-up of extracts with C18, followed by liquid chromatography-tandem mass spectrometry analysis, is proposed. A Box-Behnken design was used for optimization of the most influential variables affecting the extraction and clean-up steps. For validation, matrix-matched calibration and recovery assay were applied. Linearity (% r 2 ) higher than 99%, recoveries between 80% and 114% (except in LAS and NP1EO), RSD (precision) lower than 15% and limits of quantification between 0.03 and 12.5ngg -1 dry weight (d.w.) were achieved. The method was applied to nine samples of Holothuria collected along the coast of Granada (Spain), and to marine sediments around the animals. The results demonstrated high bioaccumulation of certain pollutants. A total of 25 out of the 36 studied compounds were quantified, being surfactants, alkylphenols, perfluoroalkyl compounds, triclocarban and parabens the most frequently detected. Nonylphenol was found in the highest concentration (340 and 323ngg -1 d.w. in sediment and Holothuria samples, respectively). Copyright © 2017 Elsevier B.V. All rights reserved.

  1. Hillasides A and B, two new cytotoxic triterpene glycosides from the sea cucumber Holothuria hilla Lesson.

    PubMed

    Wu, Jun; Yi, Yang-Hua; Tang, Hai-Feng; Wu, Hou-Ming; Zhou, Zhen-Rong

    2007-01-01

    Two new triterpene glycosides, hillasides A (1) and B (2), were isolated from the sea cucumber H. hilla Lesson, together with one known glycoside holothuria B (3). Their structures were deduced by extensive spectral analysis and chemical evidences. The presence of conjugated double bonds [22E,24-diene] in the aglycone of 1 is a rare structural feature among sea cucumber glycosides. The two glycosides showed significant cytotoxicity against eight human tumour cell lines (A-549, MCF-7, IA9, CAKI-1, PC-3, KB, KB-VIN and HCT-8) with IC(50) in the range of 0.1-3.8 microg/ml.

  2. Structural Elucidation of Novel Saponins in the Sea Cucumber Holothuria lessoni

    PubMed Central

    Bahrami, Yadollah; Zhang, Wei; Chataway, Tim; Franco, Chris

    2014-01-01

    Sea cucumbers are prolific producers of a wide range of bioactive compounds. This study aimed to purify and characterize one class of compound, the saponins, from the viscera of the Australian sea cucumber Holothuria lessoni. The saponins were obtained by ethanolic extraction of the viscera and enriched by a liquid-liquid partition process and adsorption column chromatography. A high performance centrifugal partition chromatography (HPCPC) was applied to the saponin-enriched mixture to obtain saponins with high purity. The resultant purified saponins were profiled using MALDI-MS/MS and ESI-MS/MS which revealed the structure of isomeric saponins to contain multiple aglycones and/or sugar residues. We have elucidated the structure of five novel saponins, Holothurins D/E and Holothurinosides X/Y/Z, along with seven reported triterpene glycosides, including sulfated and non-sulfated saponins containing a range of aglycones and sugar moieties, from the viscera of H. lessoni. The abundance of novel compounds from this species holds promise for biotechnological applications. PMID:25110919

  3. Purification, characterization and in vitro anticoagulant activity of polysaccharides from Gentiana scabra Bunge roots.

    PubMed

    Cai, Weirong; Xu, Huiling; Xie, Liangliang; Sun, Jian; Sun, Taotao; Wu, Xiaoyan; Fu, Qinbao

    2016-04-20

    Three water-soluble polysaccharide fractions (GSP-1, GSP-2 and GSP-3) were obtained from Gentiana scabra Bunge roots by DEAE-Sepharose CL-6B and Sepharose CL-6B column chromatography. Their chemical characterizations were determined by high performance gel permeation chromatography (HPGPC), high performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and Fourier transform infrared (FT-IR) spectrometer. Moreover, their in vitro anticoagulant activities were evaluated by activated partial thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) assays. GSP-1 and GSP-2 were composed of rhamnose, arabinose, galactose, glucose and galacturonic acid, while GSP-3 consisted of rhamnose, arabinose, galactose and galacturonic acid with a weight-average molecular weight of 5.8×10(4)Da. In comparison with the control group (saline), GSP, GSP-1, GSP-2 and GSP-3 could prolong APTT and TT, but not PT. Overall, GSP-3 exhibited potent anticoagulant activity and would be expected to be a potential source of anticoagulant. Copyright © 2015 Elsevier Ltd. All rights reserved.

  4. Antibacterial and Antifungal Activity of Holothuria leucospilota Isolated From Persian Gulf and Oman Sea

    PubMed Central

    Adibpour, Neda; Nasr, Farhad; Nematpour, Fatemeh; Shakouri, Arash; Ameri, Abdolghani

    2014-01-01

    Background: Emergence of antimicrobial resistance toward a number of conventional antibiotics has triggered the search for antimicrobial agents from a variety of sources including the marine environment. Objectives: The aim of this study was to evaluate the antimicrobial potential of Holothuria leucospilota from Qeshm and Kharg Islands against some selected bacteria and fungi. Materials and Methods: In this investigation, sea cucumbers from two coastal cities of Persian Gulf were collected in March and May 2011 and identified by the scale method according to the food and agriculture organization of the United Nations. Antibacterial activity of hydroalcoholic extracts of the body wall, cuvierian organs and coelomic fluid, methanol, chloroform, and n-hexane extracts of the body wall were evaluated by the spot test. In addition, their antifungal activity was assessed by the broth dilution method. Results: The displayed effect was microbiostatic at concentrations of 1000 and 2000 µg/mL rather than microbicidal. The highest activity of hydroalcoholic extracts was exhibited by body wall, cuvierian organs and coelomic fluid against Escherichia coli, Salmonella typhi, Staphylococcus aureus and Pseudomonas aeruginosa; Aspergillus niger, A. fumigatus, A. flavus and A. brasilensis. However, none of the methanol, chloroform and n-haxane extracts showed appreciable effects against Shigella dysenteriae, Proteus vulgaris, Bacillus cereus, S. epidermidis and Candida albicans. Moreover, cuvierian organs did not possess any antifungal potential. Conclusions: Our data indicated that water-methanol extracts from the body wall of H. leucospilota possess antibacterial and antifungal activity. However, additional and in-depth studies are required to isolate and identify the active component(s). PMID:25147657

  5. Interactions and accumulation differences of metal(loid)s in three sea cucumber species collected from the Northern Mediterranean Sea.

    PubMed

    Tunca, Evren; Aydın, Mehmet; Şahin, ÜlküAlver

    2016-10-01

    This study was conducted on Holothuria polii, Holothuria tubulosa, and Holothuria mammata collected from five stations with different depths in the Northern Mediterranean Sea. The body walls and guts of these holothurians were examined in terms of interactions of 10 metals (iron (Fe), copper (Cu), manganese (Mn), zinc (Zn), chromium (Cr), cobalt (Co), vanadium (V), nickel (Ni), cadmium (Cd), and lead (Pb)) and one metalloid (arsenic (As)) using a multivariate analysis, and interspecies differences were determined. The multivariate analysis of variance (MANOVA) revealed significant differences between the species in terms of metal(loid) accumulations. The principal component analysis (PCA) showed a more association between H. tubulosa and H. polii with regard to the accumulation. The cluster analysis (CA) located Pb concentrations of the guts to the farthest place from all elements regardless of the species. A correlation analysis displayed that the element concentrations of the guts were more closely related to each other compared with those of the walls. The most inconsistent element in terms of correlations was the gut Fe contents. Accordingly, while Fe concentrations of H. mammata and H. tubulosa were correlated with all elements (except Pb) in divalent metal transporter 1 (DMT1) (divalent cation transporter 1 (DCT1) or natural resistance-associated macrophage protein 2 (NRAMP2)) belonging to the NRAM protein family, this was not the case in H. polii. Consequently, significant relationships between accumulated metal(loid)s that changed by tissues and sea cucumber species were observed.

  6. Purification, structural characterization and anticoagulant properties of fucosylated chondroitin sulfate isolated from Holothuria mexicana.

    PubMed

    Mou, Jiaojiao; Wang, Cong; Li, Wenjing; Yang, Jie

    2017-05-01

    A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1 H and 13 C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG). The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit. The anticoagulant activities of HmG and DHmG were assessed and compared with heparin and low molecular weight heparin. The results indicated that HmG and DHmG both could significantly prolong the activated partial thrombo-plastin time, and the properties were well related to its molecular weight. DHmG showed similar anticoagulant properties to low molecular weight heparin with less bleeding risks, making it a safer anticoagulant drug. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. Structural analysis and anticoagulant activities of two sulfated polysaccharides from the sea cucumber Holothuria coluber.

    PubMed

    Yang, Wenjiao; Cai, Ying; Yin, Ronghua; Lin, Lisha; Li, Zhongkun; Wu, Mingyi; Zhao, Jinhua

    2018-05-01

    Sulfated polysaccharides such as fucosylated glycosaminoglycan and fucan sulfate from echinoderm possess complex chemical structure and various biological activities. The two sulfated polysaccharides were purified from the low-value sea cucumber Holothuria coluber. Their physicochemical properties and chemical structures were analyzed and characterized by chemical and instrumental methods. Structural analysis clarified that the sea cucumber fucosylated glycosaminoglycan contains a chondroitin sulfate-like backbone and fucosyl branches with four various sulfation patterns. The fucan sulfate with molecular weight of 64.6 kDa comprises a central core of regular α(1 → 4)-linked tetrasaccharide repeating units, each of which is linked by a 4-O-sulfated fucose residue. Anticoagulant assays indicated that these sulfated polysaccharides possessed strong APTT prolonging activities and intrinsic factor Xase inhibitory activities, both of which decreased with the reduction of their molecular weights. Our results expand knowledge on the structural types of sulfated polysaccharides from sea cucumbers and further illustrate their functionality. Copyright © 2018. Published by Elsevier B.V.

  8. Discovery of Novel Saponins from the Viscera of the Sea Cucumber Holothuria lessoni

    PubMed Central

    Bahrami, Yadollah; Zhang, Wei; Franco, Chris

    2014-01-01

    Sea cucumbers, sometimes referred to as marine ginseng, produce numerous compounds with diverse functions and are potential sources of active ingredients for agricultural, nutraceutical, pharmaceutical and cosmeceutical products. We examined the viscera of an Australian sea cucumber Holothuria lessoni Massin et al. 2009, for novel bioactive compounds, with an emphasis on the triterpene glycosides, saponins. The viscera were extracted with 70% ethanol, and this extract was purified by a liquid-liquid partition process and column chromatography, followed by isobutanol extraction. The isobutanol saponin-enriched mixture was further purified by high performance centrifugal partition chromatography (HPCPC) with high purity and recovery. The resultant purified polar samples were analyzed using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)/MS and electrospray ionization mass spectrometry (ESI-MS)/MS to identify saponins and characterize their molecular structures. As a result, at least 39 new saponins were identified in the viscera of H. lessoni with a high structural diversity, and another 36 reported triterpene glycosides, containing different aglycones and sugar moieties. Viscera samples have provided a higher diversity and yield of compounds than observed from the body wall. The high structural diversity and novelty of saponins from H. lessoni with potential functional activities presents a great opportunity to exploit their applications for industrial, agricultural and pharmaceutical use. PMID:24821624

  9. Antitumor and antifungal activities of organic extracts of seacucumber Holothuria atra from the southeast coast of India

    NASA Astrophysics Data System (ADS)

    Dhinakaran, Devaraj Isaac; Lipton, Aaron Premnath

    2015-02-01

    In phylum Echinodermata, the family Holothuridae is distinguished by its capacity of bioactive compounds. Sea cucumber Holothuria atra is commonly known as the lollyfish. The antifungal activity was detected using agar well diffusion method against the various fungal strains such as Trichoderma viride, Aspergillus niger, Aspergillus flavis, Candida albicans and Penicillium chrysogenum. Relatively high antifungal activity was seen against Candida albicans at 100 μL-1 concentration of extracts. Zone of inhibition was measured at 18 mm of diameter. The anti-tumor activities were detected against the Vero and Hep2 cell lines using MTT assay. The cells were treated with H. atra extract at concentrations 0.078-10mg mL-1. The extract showed high proliferative activity against the Hep2 cells. The body wall extracts of sea cucumber ( H. atra) showed effective antifungal and antitumor activities. All these findings suggest that the extracts could be used for the development of drugs.

  10. Hemolytic and cytotoxic properties of saponin purified from Holothuria leucospilota sea cucumber.

    PubMed

    Soltani, Mozhgan; Parivar, Kazem; Baharara, Javad; Kerachian, Mohammad Amin; Asili, Javad

    2014-10-01

    Holothuroids (sea cucumbers) are members of the phylum echinodermata, which produce saponins. Saponins exhibit a wide spectrum of pharmacological and biological activities. In this study, we isolated the crude saponins from the body wall of the dominant Iranian species of sea cucumber, Holothuria leucospilota (H. leucospilota). The purpose of this study was to confirm the presence of saponins in the Persian Gulf H. leucospilota and study the hemolytic and cytotoxic activities of these compounds. The body wall of sea cucumber was dried and powdered and the crude saponins were isolated using various solvents. The crude saponins were further purified by column chromatography using HP-20 resin. The foam test, Thin Layer Chromatography (TLC), hemolytic assay, and Fourier Transform Infrared Spectroscopy (FTIR) confirmed the presence of saponins. Cytotoxicity was analyzed using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay on A549 cells, a human lung cancer cell line. The foam test, hemolytic assay, and TLC supported the presence of saponin compounds in the 80% ethanol fraction of H. leucospilota. The infrared (IR) spectrum of the extract showed hydroxyl (-OH), alkyl (C-H), ether (C-O) and ester (-C=O) absorption characteristic of teriterpenoid saponins. The C-O-C absorption indicated glycoside linkages to the sapogenins. The crude saponin extracted from sea cucumber was cytotoxic to A549 cells. The 80% ethanol fraction of saponin isolated from H. leucospilota exhibited hemolytic activity and offers promise as an anti-cancer candidate.

  11. Spatial distribution patterns and movements of Holothuria arguinensis in the Ria Formosa (Portugal)

    NASA Astrophysics Data System (ADS)

    Siegenthaler, Andjin; Cánovas, Fernando; González-Wangüemert, Mercedes

    2015-08-01

    Holothurian populations are under pressure worldwide because of increasing demand for beche-de-mer, mainly for Asian consumption. Importations to this area from new temperate fishing grounds provide economic opportunities but also raise concerns regarding future over-exploitation. Studies on the habitat preferences and movements of sea cucumbers are important for the management of sea cucumber stocks and sizing of no-take zones, but information on the ecology and behavior of temperate sea cucumbers is scarce. This study describes the small-scale distribution and movement patterns of Holothuria arguinensis in the intertidal zone of the Ria Formosa national park (Portugal). Mark/recapture studies were performed to record their movements over time on different habitats (sand and seagrass). H. arguinensis preferred seagrass habitats and did not show a size or life stage-related spatial segregation. Its density was 563 ind. ha- 1 and mean movement speed was 10 m per day. Movement speed did not differ between habitats and the direction of movement was offshore during the day and shoreward during the night. Median home range size was 35 m2 and overlap among home ranges was 84%. H. arguinensis' high abundance, close association with seagrass and easy catchability in the intertidal zone, indicate the importance of including intertidal lagoons in future studies on temperate sea cucumber ecology since those systems might require different management strategies than fully submerged habitats.

  12. Anticancer activity and mediation of apoptosis in human HL-60 leukaemia cells by edible sea cucumber (Holothuria edulis) extract.

    PubMed

    Wijesinghe, W A J P; Jeon, You Jin; Ramasamy, Perumal; Wahid, Mohd Effendy A; Vairappan, Charles S

    2013-08-15

    Sea cucumbers have been a dietary delicacy and important ingredient in Asian traditional medicinal over many centuries. In this study, edible sea cucumber Holothuria edulis was evaluated for its in vitro anticancer potential. An aqueous fraction of the edible sea cucumber (ESC-AQ) has been shown to deliver a strong cytotoxic effect against the human HL-60 leukaemia cell line. An induction effect of apoptotic body formation in response to ESC-AQ treatment was confirmed in HL-60 cells stained with Hoechst 33342 and confirmed via flow cytometry analysis. The up regulation of Bax and caspase-3 protein expression was observed while the expression of Bcl-xL protein was down regulated in ESC-AQ treated HL-60 cells. Due to the profound anticancer activity, ESC-AQ appears to be an economically important biomass fraction that can be exploited in numerous industrial applications as a source of functional ingredients. Copyright © 2013 Elsevier Ltd. All rights reserved.

  13. Ependymin, a gene involved in regeneration and neuroplasticity in vertebrates, is overexpressed during regeneration in the echinoderm Holothuria glaberrima.

    PubMed

    Suárez-Castillo, Edna C; Medina-Ortíz, Wanda E; Roig-López, José L; García-Arrarás, José E

    2004-06-09

    We report the characterization of an ependymin-related gene (EpenHg) from a regenerating intestine cDNA library of the sea cucumber Holothuria glaberrima. This finding is remarkable because no ependymin sequence has ever been reported from invertebrates. Database comparisons of the conceptual translation of the EpenHg gene reveal 63% similarity (47% identity) with mammalian ependymin-related proteins (MERPs) and close relationship with the frog and piscine ependymins. We also report the partial sequences of ependymin representatives from another species of sea cucumber and from a sea urchin species. Conventional and real-time reverse transcriptase polymerase chain reaction (RT-PCRs) show that the gene is expressed in several echinoderm tissues, including esophagus, mesenteries, gonads, respiratory trees, hemal system, tentacles and body wall. Moreover, the ependymin product in the intestine is overexpressed during sea cucumber intestinal regeneration. The discovery of ependymins in echinoderms, a group well known for their regenerative capacities, can give us an insight on the evolution and roles of ependymin molecules.

  14. Hemolytic and cytotoxic properties of saponin purified from Holothuria leucospilota sea cucumber

    PubMed Central

    Soltani, Mozhgan; Parivar, Kazem; Baharara, Javad; Kerachian, Mohammad Amin; Asili, Javad

    2014-01-01

    Background: Holothuroids (sea cucumbers) are members of the phylum echinodermata, which produce saponins. Saponins exhibit a wide spectrum of pharmacological and biological activities. In this study, we isolated the crude saponins from the body wall of the dominant Iranian species of sea cucumber, Holothuria leucospilota (H. leucospilota). The purpose of this study was to confirm the presence of saponins in the Persian Gulf H. leucospilota and study the hemolytic and cytotoxic activities of these compounds. Methods: The body wall of sea cucumber was dried and powdered and the crude saponins were isolated using various solvents. The crude saponins were further purified by column chromatography using HP-20 resin. The foam test, Thin Layer Chromatography (TLC), hemolytic assay, and Fourier Transform Infrared Spectroscopy (FTIR) confirmed the presence of saponins. Cytotoxicity was analyzed using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay on A549 cells, a human lung cancer cell line. Results: The foam test, hemolytic assay, and TLC supported the presence of saponin compounds in the 80% ethanol fraction of H. leucospilota. The infrared (IR) spectrum of the extract showed hydroxyl (-OH), alkyl (C-H), ether (C-O) and ester (–C=O) absorption characteristic of teriterpenoid saponins. The C-O-C absorption indicated glycoside linkages to the sapogenins. The crude saponin extracted from sea cucumber was cytotoxic to A549 cells. Conclusion: The 80% ethanol fraction of saponin isolated from H. leucospilota exhibited hemolytic activity and offers promise as an anti-cancer candidate. PMID:26989736

  15. Cytotoxic Activity of Holothuria leucospilota Extract against Leishmania infantum In Vitro

    PubMed Central

    Khademvatan, Shahram; Eskandari, Alborz; Saki, Jasem; Foroutan-Rad, Masoud

    2016-01-01

    Leishmaniasis is a tropical parasitic infection. The resistance and toxicity issues are the major complications and remain significant consequences related to the treatment of leishmaniasis with the recent and classical drugs. Thus there is an immediate requirement to develop new compounds for the treatment of this protozoan disease. Sea cucumbers or holothurians are potentially presented as the marine sources of antimicrobial and cytotoxic compounds. The aim of this study was investigation of in vitro antileishmanial activity of methanol extract of body wall, coelomic fluid, and cuvierian organs of Holothuria leucospilota obtained from coastal parts of Persian Gulf against Leishmania infantum promastigotes and axenic amastigotes. The colorimetric MTT assay was used to determine L. infantum promastigotes and axenic amastigotes viability at different concentrations of the extracts and drug control (Glucantime®) at time dependent manner and the results are represented as IC50 (50% of inhibitory concentration). Coelomic fluid was the most active extract among the three different extracts of H. leucospilota against L. infantum promastigotes and axenic amastigotes with IC50s of 62.33 μg/mL and 22.4 μg/mL and 73 μg/mL and 46 μg/mL at 48 and 72 hours after treatment, respectively. Cuvierian organs extract showed less toxicity with IC50s more than 1000 μg/mL for both Leishmania infantum axenic amastigotes and promastigotes forms after 48 and 72 hours of exposure. Results acquired from the present study propose that the sea cucumber H. leucospilota may be a provoking source of antileishmanial compounds and could be a lead source in the development of the potent antileishmanial and cytotoxic drugs. PMID:27022392

  16. Sulfated polysaccharides with antioxidant and anticoagulant activity from the sea cucumber Holothuria fuscogliva

    NASA Astrophysics Data System (ADS)

    Li, Rongfeng; Yu, Huahua; Yue, Yang; Liu, Song; Xing, Rong'e.; Chen, Xiaolin; Li, Pengcheng

    2017-07-01

    Sea cucumber is a traditional nutritional food and medicinal resource with many bioactive components in China. Holothuria fuscogliva is a big sea cucumber with a rich of bioactive polysaccharides. To investigate the bioactivities of the polysaccharides from sea cucumber H. fuscogliva, we prepared the sulfated polysaccharides (HfP) from sea cucumber H. fuscogliva using a protease hydrolysis method. Antioxidant activities of HfP were investigated, including hydroxyl radical scavenging activity and superoxide radical scavenging activity. And, the anticoagulant activities of HfP were studied, including the activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT). The average molecular weight was 1 867.1 Da, with a sulfate content of 20.7%. In addition, the molar ratio of monosaccharide composition of HfP was Man: Rha: Glc A: Glc: Gal: Xyl: Fuc=0.083 6: 0.437: 0.134: 0:1.182: 0.748: 1. It had a strong antioxidant activity, the hydroxyl and superoxide radical scavenging activity EC50 of HfP was 3.74 and 0.037 mg/mL, respectively. It also showed a good anticoagulant activity in our study. The APTT of HfP was much higher than that of heparin sodium, and the PT and TT of HfP was close to that of heparin sodium at a low concentration. Therefore, HfP shows a good antioxidant and anticoagulant activity and it may become a potential candidate of the natural antioxidant and anticoagulant and will have a good application future in health product or medicine industry.

  17. Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance

    PubMed Central

    Xu, Yi; Burgess, Patrick; Huang, Bingru

    2015-01-01

    Mechanisms of plant root tolerance to high temperatures through antioxidant defense are not well understood. The objective of this study was to investigate whether superior root thermotolerance of heat-tolerant Agrostis scabra relative to its congeneric heat-sensitive Agrostis stolonifera was associated with differential accumulation of reactive oxygen species and antioxidant scavenging systems. A. scabra ‘NTAS’ and A. stolonifera ‘Penncross’ plants were exposed to heat stress (35/30°C, day/night) in growth chambers for 24 d. Superoxide (O2 -) content increased in both A. stolonifera and A. scabra roots under heat stress but to a far lesser extent in A. scabra than in A. stolonifera. Hydrogen peroxide (H2O2) content increased significantly in A. stolonifera roots but not in A. scabra roots responding to heat stress. The content of antioxidant compounds (ascorbate and glutathione) did not differ between A. stolonifera and A. scabra under heat stress. Enzymatic activity of superoxide dismutase was less suppressed in A. scabra than that in A. stolonifera under heat stress, while peroxidase and catalase were more induced in A. scabra than in A. stolonifera. Similarly, their encoded transcript levels were either less suppressed, or more induced in A. scabra roots than those in A. stolonifera during heat stress. Roots of A. scabra exhibited greater alternative respiration rate and lower cytochrome respiration rate under heat stress, which was associated with suppression of O2 - and H2O2 production as shown by respiration inhibitors. Superior root thermotolerance of A. scabra was related to decreases in H2O2 and O2 - accumulation facilitated by active enzymatic antioxidant defense systems and the maintenance of alternative respiration, alleviating cellular damages by heat-induced oxidative stress. PMID:26382960

  18. Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance.

    PubMed

    Xu, Yi; Burgess, Patrick; Huang, Bingru

    2015-01-01

    Mechanisms of plant root tolerance to high temperatures through antioxidant defense are not well understood. The objective of this study was to investigate whether superior root thermotolerance of heat-tolerant Agrostis scabra relative to its congeneric heat-sensitive Agrostis stolonifera was associated with differential accumulation of reactive oxygen species and antioxidant scavenging systems. A. scabra 'NTAS' and A. stolonifera 'Penncross' plants were exposed to heat stress (35/30°C, day/night) in growth chambers for 24 d. Superoxide (O2(-)) content increased in both A. stolonifera and A. scabra roots under heat stress but to a far lesser extent in A. scabra than in A. stolonifera. Hydrogen peroxide (H2O2) content increased significantly in A. stolonifera roots but not in A. scabra roots responding to heat stress. The content of antioxidant compounds (ascorbate and glutathione) did not differ between A. stolonifera and A. scabra under heat stress. Enzymatic activity of superoxide dismutase was less suppressed in A. scabra than that in A. stolonifera under heat stress, while peroxidase and catalase were more induced in A. scabra than in A. stolonifera. Similarly, their encoded transcript levels were either less suppressed, or more induced in A. scabra roots than those in A. stolonifera during heat stress. Roots of A. scabra exhibited greater alternative respiration rate and lower cytochrome respiration rate under heat stress, which was associated with suppression of O2(-) and H2O2 production as shown by respiration inhibitors. Superior root thermotolerance of A. scabra was related to decreases in H2O2 and O2(-) accumulation facilitated by active enzymatic antioxidant defense systems and the maintenance of alternative respiration, alleviating cellular damages by heat-induced oxidative stress.

  19. Antioxidant and cytotoxic properties of two sea cucumbers, Holothuria edulis lesson and Stichopus horrens Selenka.

    PubMed

    Althunibat, O Y; Ridzwan, B H; Taher, M; Daud, J M; Jauhari Arief Ichwan, S; Qaralleh, H

    2013-03-01

    Sea cucumbers are marine invertebrates of the phylum of Echinodermata that have been used in Asian traditional medicine since ancient times. This study was conducted to investigate the antioxidant and cytotoxic properties of aqueous and organic extracts from two sea cucumber species, Holothuria edulis Lesson (Holothuriidae) and Stichopus horrens Selenka (Stichopodidae). Antioxidant activities of the extracts were evaluated by DPPH· and β-carotene bleaching assays, while MTT and trypan blue exclusion assays were used to demonstrate the cytotoxic effects of the extracts against two human cancer cell lines, non-small cell lung cancer cells (A549) and esophageal cancer cells (TE1). The results showed that both aqueous and organic extracts of H. edulis were able to scavenge DPH radical (IC50 at 2.04 mg/ml and 8.73 mg/ml, respectively). Aqueous and organic extracts of S. horrens inhibited 79.62% and 46.66% of β-carotene oxidation by linoleate free radical. On the other hand, the organic extract of S. horrens exhibited the highest cytotoxic effects against A549 and TE1 cancer cells giving IC50 at 15.5 and 4.0 μg/ml, respectively. In conclusion, the present study revealed that H. edulis and S. horrens contain promising levels of antioxidant and cytotoxic natural products that might be used for cancer prevention and treatment.

  20. Prevalence of Haplorchis taichui in Field-Collected Snails: A Molecular Approach

    PubMed Central

    Chontananarth, Thapana

    2010-01-01

    The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda: Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988 snails were collected from Mae Taeng district, Chiang Mai province, northern Thailand, which comprised of 3 species; Melanoides tuberculata, Tarebia granifera, and Thiara scabra. The overall prevalence of pleurolophocercous cercariae was 21.7% as determined by the morphology. For genetic detection of H. taichui infection in snails, 2 primers Hapt_F (5'-GGCCAACGCAATCGTCATCC-3') and Hapt_R (5'-GCGTCGGGTTTCAGACATGG-3'), were used. The genomic DNA of H. taichui, which was used as a positive control, gave an amplification of the 256 bp fragment. The overall prevalence of H. taichui from specific PCR was 9.7%. The proportion of H. taichui among the pleurolophocercous cercariae in this study was 44.9%. PMID:21234240

  1. Isolation and Characterization of Adhesive Secretion from Cuvierian Tubules of Sea Cucumber Holothuria forskåli (Echinodermata: Holothuroidea)

    PubMed Central

    Baranowska, Malgorzata; Schloßmacher, Ute; McKenzie, J. Douglas; Müller, Werner E. G.; Schröder, Heinz C.

    2011-01-01

    The sea cucumber Holothuria forskåli possesses a specialized system called Cuvierian tubules. During mechanical stimulation white filaments (tubules) are expelled and become sticky upon contact with any object. We isolated a protein with adhesive properties from protein extracts of Cuvierian tubules from H. forskåli. This protein was identified by antibodies against recombinant precollagen D which is located in the byssal threads of the mussel Mytilus galloprovincialis. To find out the optimal procedure for extraction and purification, the identified protein was isolated by several methods, including electroelution, binding to glass beads, immunoprecipitation, and gel filtration. Antibodies raised against the isolated protein were used for localization of the adhesive protein in Cuvierian tubules. Immunostaining and immunogold electron microscopical studies revealed the strongest immunoreactivity in the mesothelium; this tissue layer is involved in adhesion. Adhesion of Cuvierian tubule extracts was measured on the surface of various materials. The extracted protein showed the strongest adhesion to Teflon surface. Increased adhesion was observed in the presence of potassium and EDTA, while cadmium caused a decrease in adhesion. Addition of antibodies and trypsin abolished the adhesive properties of the extract. PMID:22013488

  2. Profiling bacterial communities associated with sediment-based aquaculture bioremediation systems under contrasting redox regimes

    NASA Astrophysics Data System (ADS)

    Robinson, Georgina; Caldwell, Gary S.; Wade, Matthew J.; Free, Andrew; Jones, Clifford L. W.; Stead, Selina M.

    2016-12-01

    Deposit-feeding invertebrates are proposed bioremediators in microbial-driven sediment-based aquaculture effluent treatment systems. We elucidate the role of the sediment reduction-oxidation (redox) regime in structuring benthic bacterial communities, having direct implications for bioremediation potential and deposit-feeder nutrition. The sea cucumber Holothuria scabra was cultured on sediments under contrasting redox regimes; fully oxygenated (oxic) and redox stratified (oxic-anoxic). Taxonomically, metabolically and functionally distinct bacterial communities developed between the redox treatments with the oxic treatment supporting the greater diversity; redox regime and dissolved oxygen levels were the main environmental drivers. Oxic sediments were colonised by nitrifying bacteria with the potential to remediate nitrogenous wastes. Percolation of oxygenated water prevented the proliferation of anaerobic sulphate-reducing bacteria, which were prevalent in the oxic-anoxic sediments. At the predictive functional level, bacteria within the oxic treatment were enriched with genes associated with xenobiotics metabolism. Oxic sediments showed the greater bioremediation potential; however, the oxic-anoxic sediments supported a greater sea cucumber biomass. Overall, the results indicate that bacterial communities present in fully oxic sediments may enhance the metabolic capacity and bioremediation potential of deposit-feeder microbial systems. This study highlights the benefits of incorporating deposit-feeding invertebrates into effluent treatment systems, particularly when the sediment is oxygenated.

  3. Profiling bacterial communities associated with sediment-based aquaculture bioremediation systems under contrasting redox regimes

    PubMed Central

    Robinson, Georgina; Caldwell, Gary S.; Wade, Matthew J.; Free, Andrew; Jones, Clifford L. W.; Stead, Selina M.

    2016-01-01

    Deposit-feeding invertebrates are proposed bioremediators in microbial-driven sediment-based aquaculture effluent treatment systems. We elucidate the role of the sediment reduction-oxidation (redox) regime in structuring benthic bacterial communities, having direct implications for bioremediation potential and deposit-feeder nutrition. The sea cucumber Holothuria scabra was cultured on sediments under contrasting redox regimes; fully oxygenated (oxic) and redox stratified (oxic-anoxic). Taxonomically, metabolically and functionally distinct bacterial communities developed between the redox treatments with the oxic treatment supporting the greater diversity; redox regime and dissolved oxygen levels were the main environmental drivers. Oxic sediments were colonised by nitrifying bacteria with the potential to remediate nitrogenous wastes. Percolation of oxygenated water prevented the proliferation of anaerobic sulphate-reducing bacteria, which were prevalent in the oxic-anoxic sediments. At the predictive functional level, bacteria within the oxic treatment were enriched with genes associated with xenobiotics metabolism. Oxic sediments showed the greater bioremediation potential; however, the oxic-anoxic sediments supported a greater sea cucumber biomass. Overall, the results indicate that bacterial communities present in fully oxic sediments may enhance the metabolic capacity and bioremediation potential of deposit-feeder microbial systems. This study highlights the benefits of incorporating deposit-feeding invertebrates into effluent treatment systems, particularly when the sediment is oxygenated. PMID:27941918

  4. Protective and Curative Effects of the Sea Cucumber Holothuria atra Extract against DMBA-Induced Hepatorenal Diseases in Rats

    PubMed Central

    Dakrory, Ahmed I.; Fahmy, Sohair R.; Soliman, Amel M.; Mohamed, Ayman S.; Amer, Sayed A. M.

    2015-01-01

    Oxidative stress is a common mechanism contributing to the initiation and progression of hepatic damage. Hence there is a great demand for the development of agents with potent antioxidant effect. The aim of the present study is to evaluate the efficacy of Holothuria atra extract (HaE) as an antioxidant against 7,12-dimethylbenz[a]anthracene- (DMBA-) induced hepatorenal dysfunction. Experimental animals were divided into two main groups: protective and curative. Each group was then divided into five subgroups pre- or posttreated either with distilled water (DMBA subgroups) or with HaE (200 mg/kg body weight) for seven and fourteen days. Single oral administration of DMBA (15 mg/kg body weight) to Wistar rats resulted in a significant increase in the serum liver enzymes and kidney function's parameters. DMBA increased level of liver malondialdehyde (MDA), decreased levels of reduced glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT) in the liver tissue, and induced liver histopathological alterations. Pre- or posttreatment with HaE orally for 14 days significantly reversed the hepatorenal alterations induced following DMBA administration. In conclusion, HaE exhibits good hepatoprotective, curative, and antioxidant potential against DMBA-induced hepatorenal dysfunction in rats that might be due to decreased free radical generation. PMID:25821811

  5. In vitro decondensation of the sperm chromatin in Holothuria tubulosa (sea cucumber) not affecting proteolysis of basic nuclear proteins.

    PubMed

    del Valle, Luis J

    2005-06-01

    Sea urchin and sea star oocyte extracts contain proteolytic activities that are active against sperm basic nuclear proteins (SNBP). This SNBP degradation has been related to the decondensation of sperm chromatin as a possible model to male pronuclei formation. We have studied the presence of this proteolytic activity in Holothuria tubulosa (sea cucumber) and its possible relationship with sperm nuclei decondensation. The mature oocyte extracts from H. tubulosa contain a proteolytic activity to SNBP located in the macromolecular fraction of the egg-jelly layer. SNBP degradation occurred both on sperm nuclei and on purified SNBP, histones being more easily degraded than protein Ø(o) (sperm-specific protein). SNBP degradation was found to be dependent on concentration, incubation time, presence of Ca(2+), pH, and this activity could be a serine-proteinase. Thermal denaturalization of the oocyte extracts (80 degrees C, 10-15 min) inactivates its proteolytic activity on SNBP but does not affect sperm nuclei decondensation. These results would suggest that sperm nuclei decondensation occurs by a mechanism different from SNBP degradation. Thus, the sperm nuclei decondensation occurs by a thermostable factor(s) and the removal of linker SNBP (H1 and protein Ø(o)) will be a first condition in the process of sperm chromatin remodeling.

  6. Identification of endocannabinoid system-modulating N-alkylamides from Heliopsis helianthoides var. scabra and Lepidium meyenii.

    PubMed

    Hajdu, Zsanett; Nicolussi, Simon; Rau, Mark; Lorántfy, László; Forgo, Peter; Hohmann, Judit; Csupor, Dezső; Gertsch, Jürg

    2014-07-25

    The discovery of the interaction of plant-derived N-alkylamides (NAAs) and the mammalian endocannabinoid system (ECS) and the existence of a plant endogenous N-acylethanolamine signaling system have led to the re-evaluation of this group of compounds. Herein, the isolation of seven NAAs and the assessment of their effects on major protein targets in the ECS network are reported. Four NAAs, octadeca-2E,4E,8E,10Z,14Z-pentaene-12-ynoic acid isobutylamide (1), octadeca-2E,4E,8E,10Z,14Z-pentaene-12-ynoic acid 2'-methylbutylamide (2), hexadeca-2E,4E,9Z-triene-12,14-diynoic acid isobutylamide (3), and hexadeca-2E,4E,9,12-tetraenoic acid 2'-methylbutylamide (4), were identified from Heliopsis helianthoides var. scabra. Compounds 2-4 are new natural products, while 1 was isolated for the first time from this species. The previously described macamides, N-(3-methoxybenzyl)-(9Z,12Z,15Z)-octadecatrienamide (5), N-benzyl-(9Z,12Z,15Z)-octadecatrienamide (6), and N-benzyl-(9Z,12Z)-octadecadienamide (7), were isolated from Lepidium meyenii (Maca). N-Methylbutylamide 4 and N-benzylamide 7 showed submicromolar and selective binding affinities for the cannabinoid CB1 receptor (Ki values of 0.31 and 0.48 μM, respectively). Notably, compound 7 also exhibited weak fatty acid amide hydrolase (FAAH) inhibition (IC50 = 4 μM) and a potent inhibition of anandamide cellular uptake (IC50 = 0.67 μM) that was stronger than the inhibition obtained with the controls OMDM-2 and UCM707. The pronounced ECS polypharmacology of compound 7 highlights the potential involvement of the arachidonoyl-mimicking 9Z,12Z double-bond system in the linoleoyl group for the overall cannabimimetic action of NAAs. This study provides additional strong evidence of the endocannabinoid substrate mimicking of plant-derived NAAs and uncovers a direct and indirect cannabimimetic action of the Peruvian Maca root.

  7. Distinct profiles of expressed sequence tags during intestinal regeneration in the sea cucumber Holothuria glaberrima

    PubMed Central

    Rojas-Cartagena, Carmencita; Ortíz-Pineda, Pablo; Ramírez-Gómez, Francisco; Suárez-Castillo, Edna C.; Matos-Cruz, Vanessa; Rodríguez, Carlos; Ortíz-Zuazaga, Humberto; García-Arrarás, José E.

    2010-01-01

    Repair and regeneration are key processes for tissue maintenance, and their disruption may lead to disease states. Little is known about the molecular mechanisms that underline the repair and regeneration of the digestive tract. The sea cucumber Holothuria glaberrima represents an excellent model to dissect and characterize the molecular events during intestinal regeneration. To study the gene expression profile, cDNA libraries were constructed from normal, 3-day, and 7-day regenerating intestines of H. glaberrima. Clones were randomly sequenced and queried against the nonredundant protein database at the National Center for Biotechnology Information. RT-PCR analyses were made of several genes to determine their expression profile during intestinal regeneration. A total of 5,173 sequences from three cDNA libraries were obtained. About 46.2, 35.6, and 26.2% of the sequences for the normal, 3-days, and 7-days cDNA libraries, respectively, shared significant similarity with known sequences in the protein database of GenBank but only present 10% of similarity among them. Analysis of the libraries in terms of functional processes, protein domains, and most common sequences suggests that a differential expression profile is taking place during the regeneration process. Further examination of the expressed sequence tag dataset revealed that 12 putative genes are differentially expressed at significant level (R > 6). Experimental validation by RT-PCR analysis reveals that at least three genes (unknown C-4677-1, melanotransferrin, and centaurin) present a differential expression during regeneration. These findings strongly suggest that the gene expression profile varies among regeneration stages and provide evidence for the existence of differential gene expression. PMID:17579180

  8. Identification of (Z)-2,3-Diphenylacrylonitrile as Anti-Cancer Molecule in Persian Gulf Sea Cucumber Holothuria parva

    PubMed Central

    Amidi, Salimeh; Hashemi, Zahra; Motallebi, Abbasali; Nazemi, Melika; Farrokhpayam, Hoda; Seydi, Enayatollah

    2017-01-01

    Hepatocellular carcinoma (HCC), also named cancerous hepatoma, is the most common type of malignant neoplasia of the liver. In this research, we screened the Persian Gulf sea cucumber Holothuria parva (H. parva) methanolic sub-fractions for the possible existence of selective toxicity on liver mitochondria isolated from an animal model of HCC. Next, we purified the most active fraction. Thus the structure of the active molecule was identified. HCC was induced by diethylnitrosamine (DEN) and 2-acetylaminofluorene (2-AAF) protocol. Rat liver mitochondria for evaluation of the selective cytotoxic effects of sub-fractions of H. parva were isolated and then mitochondrial parameters were determined. Our results showed that C1 sub-fraction of methanolic extract of H. parva considerably increased reactive oxygen species (ROS) generation, collapse of mitochondrial membrane potential (MMP), swelling in mitochondria and cytochrome c release only on HCC liver mitochondria. Furthermore, the methanolic extract of H. parva was investigated furthermore and the active fraction was extracted. In this fraction, (Z)-2,3-diphenylacrylonitrile molecule, which is also known as α-cyanostilbene, was identified by mass analysis. This molecule increased ROS generation, collapse of MMP, swelling in mitochondria and finally cytochrome c release only on HCC liver mitochondria. The derivatives of (Z)-2,3-diphenylacrylonitrile in other natural products were also reported as an anti-cancer agent. These results suggest the eligibility of the (Z)-2,3-diphenylacrylonitrile as a complementary therapeutic agent for patients with HCC. PMID:29035293

  9. Molecular characterization and gene expression patterns of retinoid receptors, in normal and regenerating tissues of the sea cucumber, Holothuria glaberrima.

    PubMed

    Viera-Vera, Jorge; García-Arrarás, José E

    2018-05-15

    Retinoic acid receptors (RAR) and retinoid X receptors (RXR) are ligand-mediated transcription factors that synchronize intricate signaling networks in metazoans. Dimer formation between these two nuclear receptors mediates the recruitment of co-regulatory complexes coordinating the progression of signaling cascades during developmental and regenerative events. In the present study we identified and characterized the receptors for retinoic acid in the sea cucumber Holothuria glaberrima; a model system capable of regenerative organogenesis during adulthood. Molecular characterizations revealed the presence of three isoforms of RAR and two of RXR as a consequence of alternative splicing events. Various analyses including: primary structure sequencing, phylogenetic analysis, protein domain prediction, and multiple sequence alignment further confirmed their identity. Semiquantitative reverse transcription PCR analysis of each receptor isoform herein identified showed that the retinoid receptors are expressed in all tissues sampled: the mesenteries, respiratory trees, muscles, gonads, and the digestive tract. During regenerative organogenesis two of the receptors (RAR-L and RXR-T) showed differential expression in the posterior segment while RAR-S is differentially expressed in the anterior segment of the intestine. This work presents the first description of the components relaying the signaling for retinoic acid within this model system. Copyright © 2018 Elsevier B.V. All rights reserved.

  10. Value, Market Preferences and Trade of Beche-De-Mer from Pacific Island Sea Cucumbers

    PubMed Central

    Purcell, Steven W.

    2014-01-01

    Market preferences of natural resources contribute to shape their exploitation and production. Beche-de-mer, the product after gutting, cooking, salting and drying sea cucumbers, is exported worldwide to Asian dried seafood markets. A better understanding of the trade, value and market preferences of Pacific island beche-de-mer could identify critical postharvest processing techniques and management strategies for fisheries and aquaculture. Data were collected on export prices and trade of beche-de-mer from Kiribati, Fiji, Tonga and New Caledonia, and the selling prices, respective sizes and organoleptic properties of the products in stores in China. Export prices varied considerably within and among the four countries and low-value species were the most exported by volume. Most of the beche-de-mer from the four Pacific islands is exported to Hong Kong, where quality products are sold and others are distributed to mainland China. Prices of the beche-de-mer in Chinese stores varied up to ten-fold and were mostly influenced by species, body size and, to a lesser extent, physical damage to the products. Market prices across species (averaging US$15–385 kg−1) appear to have mostly increased six- to twelve-fold over the past decade. The data allude that fisheries for Holothuria scabra, H. lessoni, H. fuscogilva, H. whitmaei and Thelenota ananas should be most carefully managed because they were the highest-value species and under greatest demand. The relationships between size of beche-de-mer and sale price were species specific and highly varied. This study also highlights the need for better regulations and/or enforcement of minimum size limits in sea cucumber fisheries, which can help to maximise economic benefits of wild stocks. PMID:24736374

  11. Value, market preferences and trade of Beche-de-mer from Pacific Island sea cucumbers.

    PubMed

    Purcell, Steven W

    2014-01-01

    Market preferences of natural resources contribute to shape their exploitation and production. Beche-de-mer, the product after gutting, cooking, salting and drying sea cucumbers, is exported worldwide to Asian dried seafood markets. A better understanding of the trade, value and market preferences of Pacific island beche-de-mer could identify critical postharvest processing techniques and management strategies for fisheries and aquaculture. Data were collected on export prices and trade of beche-de-mer from Kiribati, Fiji, Tonga and New Caledonia, and the selling prices, respective sizes and organoleptic properties of the products in stores in China. Export prices varied considerably within and among the four countries and low-value species were the most exported by volume. Most of the beche-de-mer from the four Pacific islands is exported to Hong Kong, where quality products are sold and others are distributed to mainland China. Prices of the beche-de-mer in Chinese stores varied up to ten-fold and were mostly influenced by species, body size and, to a lesser extent, physical damage to the products. Market prices across species (averaging US$15-385 kg-1) appear to have mostly increased six- to twelve-fold over the past decade. The data allude that fisheries for Holothuria scabra, H. lessoni, H. fuscogilva, H. whitmaei and Thelenota ananas should be most carefully managed because they were the highest-value species and under greatest demand. The relationships between size of beche-de-mer and sale price were species specific and highly varied. This study also highlights the need for better regulations and/or enforcement of minimum size limits in sea cucumber fisheries, which can help to maximise economic benefits of wild stocks.

  12. Morphological and Molecular Identification of Holothuria (Merthensiothuria) leucospilota and Stichopus horrens from Pangkor Island, Malaysia

    PubMed Central

    Kamarudin, Kamarul Rahim; Rehan, Maryam Mohamed

    2015-01-01

    This preliminary study aimed to identify a commercial gamat species, Stichopus horrens Selenka, 1867, and a timun laut species, Holothuria (Mertensiothuria) leucospilota (Brandt, 1835), from Pangkor Island, Perak, Malaysia, employing morphological techniques based on the shape of the ossicles and molecular techniques based on the cytochrome c oxidase I (COI) mitochondrial DNA (mtDNA) gene. In Malaysia, a gamat is defined as a sea cucumber species of the family Stichopodidae with medicinal value, and timun laut refers to non-gamat species. S. horrens is very popular on Pangkor Island as a main ingredient in the traditional production of air gamat and minyak gamat, while H. leucospilota is the most abundant species in Malaysia. In contrast to previous studies, internal body parts (the respiratory tree and gastrointestine) were examined in this study to obtain better inferences based on morphology. The results showed that there were no ossicles present in the gastrointestine of H. leucospilota, and this characteristic is suggested as a unique diagnostic marker for the timun laut species. In addition, the presence of Y-shaped rods in the respiratory tree of S. horrens subsequently supported the potential to use internal body parts to identify the gamat species. Phylogenetic analysis of the COI mtDNA gene of the sea cucumber specimens using the neighbour-joining method and maximum likelihood methods further confirmed the species status of H. leucospilota and S. horrens from Pangkor Island, Perak, Malaysia. The COI mtDNA gene sequences were registered with GenBank, National Center for Biotechnology Information (NCBI), US National Library of Medicine (GenBank accession no.: KC405565-KC405568). Although additional specimens from various localities will be required to produce more conclusive results, the current findings provide better insight into the importance of complementary approaches involving morphological and molecular techniques in the identification of the two

  13. Morphological and Molecular Identification of Holothuria (Merthensiothuria) leucospilota and Stichopus horrens from Pangkor Island, Malaysia.

    PubMed

    Kamarudin, Kamarul Rahim; Rehan, Maryam Mohamed

    2015-04-01

    This preliminary study aimed to identify a commercial gamat species, Stichopus horrens Selenka, 1867, and a timun laut species, Holothuria (Mertensiothuria) leucospilota (Brandt, 1835), from Pangkor Island, Perak, Malaysia, employing morphological techniques based on the shape of the ossicles and molecular techniques based on the cytochrome c oxidase I (COI) mitochondrial DNA (mtDNA) gene. In Malaysia, a gamat is defined as a sea cucumber species of the family Stichopodidae with medicinal value, and timun laut refers to non-gamat species. S. horrens is very popular on Pangkor Island as a main ingredient in the traditional production of air gamat and minyak gamat, while H. leucospilota is the most abundant species in Malaysia. In contrast to previous studies, internal body parts (the respiratory tree and gastrointestine) were examined in this study to obtain better inferences based on morphology. The results showed that there were no ossicles present in the gastrointestine of H. leucospilota, and this characteristic is suggested as a unique diagnostic marker for the timun laut species. In addition, the presence of Y-shaped rods in the respiratory tree of S. horrens subsequently supported the potential to use internal body parts to identify the gamat species. Phylogenetic analysis of the COI mtDNA gene of the sea cucumber specimens using the neighbour-joining method and maximum likelihood methods further confirmed the species status of H. leucospilota and S. horrens from Pangkor Island, Perak, Malaysia. The COI mtDNA gene sequences were registered with GenBank, National Center for Biotechnology Information (NCBI), US National Library of Medicine (GenBank accession no.: KC405565-KC405568). Although additional specimens from various localities will be required to produce more conclusive results, the current findings provide better insight into the importance of complementary approaches involving morphological and molecular techniques in the identification of the two

  14. Apoptosis inducing capacity of Holothuria arenicola in CT26 colon carcinoma cells in vitro and in vivo

    PubMed Central

    Baharara, Javad; Amini, Elaheh; Afzali, Mahbubeh; Nikdel, Najme; Mostafapour, Asma; Kerachian, Mohammad Amin

    2016-01-01

    Objective(s): Sea cucumber is one of the classes of echinoderms, which is considered as a health marine product and possess various biological characteristics with therapeutic application. The present investigation attempted to evaluate the potential of anti-cancer Persian Gulf sea cucumber species Holothuria arenicola (H. arenicola) aqueous extract on mice colon carcinoma cells in vitro and in vivo. Materials and Methods: The CT26 carcinoma cells were treated with various concentrations of extract in 24 and 48 hr, and then its anti-proliferative effect was measured by MTT assay and morphological observations. The apoptotic effect was examined by fluorescence microscopy (DNA fragmentation assay), Flow cytometry, caspase-3 and -9 colorimetric assays. The in vivo anti-tumor efficacy of sea cucumber extract on CT26 tumor cells transplanted in BALB/c mice was also investigated. Results: The results showed that the water extract of sea cucumber revealed remarkable anti-proliferative effect on CT26 tumor cells with IC50= 31 µg/ml with recruitment of intrinsic apoptotic pathway in vitro. In addition, the colon tumor volume in treated groups remarkably reduced in homozygous mice. Histopathological examination elucidated that sea cucumber extract attenuated tumor size and volume along with apoptosis characteristics. Moreover, RT-PCR analysis revealed that sea cucumber extract induced intrinsic apoptosis in vivo through suppression of Bcl-2 expression. Conclusion: Our data confirmed this notion that sea cucumber administrates anti-cancer effect that can be used as complementary in preclinical experiments, so further characterization are recommended for detection sea cucumber metabolites and clinical application. PMID:27279978

  15. Abundance of sea cucumbers on the ecosystem of seagrasses Inunggeh island, Tapanuli Tengah Regency North Sumatera Province

    NASA Astrophysics Data System (ADS)

    Wisesa, M. M.; Bakti, D.; Fadhilah, A.

    2018-02-01

    Unggeh Island is one area that has the potential of Sea Cucumber in the North Sumatra. Sea cucumbers have an important role in ecosystem waters, namely as a deposit feeder. Sea cucumbers can live in shallow waters, such as seagrass ecosystems. The purpose of this study is to knowing the abundance of sea cucumbers in the seagrass ecosystems on the island of Unggeh and to knowing the type of Sea Cucumber. The method used is a transect quadrant method with a size of 5x5 meters, on a transect line with a length of 100 meters. Sampling was done at three points observations, station 1 was at coordinate point 01°34’26,88 "LU and 098°45’40,25" BT, station 2 was at coordinate point 01°34’32,71 "LU and 098°45’37, 58 "BT, station 3 is at the coordinate point 01°34’24,22" LU and 098°45’38,06 "BT. The type of sea cucumber found in the seagrass ecosystem on the Unggeh island Actinopyga ecinites, A. Miliaris, Holothuria scabra. The density at station 1 was 0.16 ind / m2, at station II a density was0.12 ind / m2, at station III a density was 0.08 ind / m2, and the total density at the research location was 0, 32 ind / m2.

  16. Bioactive compounds, antioxidant potential, and hepatoprotective activity of sea cucumber (Holothuria atra) against thioacetamide intoxication in rats.

    PubMed

    Esmat, Amr Y; Said, Mahmoud M; Soliman, Amel A; El-Masry, Khaled S H; Badiea, Elham Abdel

    2013-01-01

    The identification of the active phenolic compounds in the mixed extract of sea cucumber (Holothuria atra) body wall by high-performance liquid chromatography and an assessment of its hepatoprotective activity against thioacetamide-induced liver fibrosis in rats. Female Swiss albino rats were divided into four groups: normal controls; oral administration of a sea cucumber mixed extract (14.4 mg/kg of body weight) on days 2, 4, and 6 weekly for 8 consecutive weeks; intoxication with thioacetamide (200 mg/kg of body weight, intraperitoneally) on days 2 and 6 weekly for 8 wk; and oral administration of a sea cucumber extract and then intoxication with thioacetamide 2 h later for 8 wk. High-performance liquid chromatographic analysis of the sea cucumber mixed extract revealed the presence of some phenolic components, such as chlorogenic acid, pyrogallol, rutin, coumaric acid, catechin, and ascorbic acid. In vitro studies have shown that the extract has a high scavenging activity for the nitric oxide radical, a moderate iron-chelating activity, and a weak inhibitory effect of lipid peroxidation. The subchronic oral administration of sea cucumber extract to the rats did not show any toxic side effects but increased hepatic superoxide dismutase and glutathione peroxidase activities. The coadministration of sea cucumber extract and thioacetamide (protection modality) normalized serum direct bilirubin, alanine and aspartate aminotransferases, hepatic malondialdehyde, and hydroxyproline concentrations and antioxidant enzyme activities. In addition, the histologic examination of liver sections from the protection group that were stained with hematoxylin and eosin showed substantial attenuation of the degenerative cellular changes and regressions in liver fibrosis and necrosis induced by the thioacetamide intoxication. Sea cucumber mixed extract contains physiologically active phenolic compounds with antioxidant activity, which afforded a potential hepatoprotective activity

  17. Megadenus atrae n. sp., an endoparasitic eulimid gastropod (Mollusca) from the black sea cucumber Holothuria atra Jaeger (Aspidochirotida: Holothuriidae) in the Indo-West Pacific.

    PubMed

    Takano, Tsuyoshi; Warén, Anders; Kano, Yasunori

    2017-07-01

    An eulimid gastropod, Megadenus atrae n. sp., endoparasitic in the cloacal chamber of the black sea cucumber Holothuria atra Jaeger is described from Okinawa, Japan, as the fifth species of the genus. Conspecific specimens have also been found from southeast India, northeast Australia and New Caledonia. The generic assignment is justified by the presence of (i) a thick, long proboscis that bears a large fold (pseudopallium) near the base and a collar-like structure at the middle, (ii) a thin, globose shell that is covered by the pseudopallium, and (iii) sexual dimorphism with the female generally larger than the male. The new species is distinguishable from the four previously described congeners by its cauldron-shaped pseudopallium, moderately-developed collar of the proboscis and rounded basal lip of the shell. The comparisons of the size and sex of solitary and paired individuals support a previous hypothesis that the species of Megadenus Rosén, 1910 are protandrous with environmental sex determination. The present species occurs mostly as monogamous pairs despite its very low population density, implying that the presence of a conspecific individual acts as a cue for larval settlement. Both mechanisms would increase individual reproductive success in such permanent parasites with low prevalence and abundance as the species of Megadenus.

  18. Common cellular events occur during wound healing and organ regeneration in the sea cucumber Holothuria glaberrima.

    PubMed

    San Miguel-Ruiz, José E; García-Arrarás, José E

    2007-10-18

    All animals possess some type of tissue repair mechanism. In some species, the capacity to repair tissues is limited to the healing of wounds. Other species, such as echinoderms, posses a striking repair capability that can include the replacement of entire organs. It has been reported that some mechanisms, namely extracellular matrix remodeling, appear to occur in most repair processes. However, it remains unclear to what extent the process of organ regeneration, particularly in animals where loss and regeneration of complex structures is a programmed natural event, is similar to wound healing. We have now used the sea cucumber Holothuria glaberrima to address this question. Animals were lesioned by making a 3-5 mm transverse incision between one of the longitudinal muscle pairs along the bodywall. Lesioned tissues included muscle, nerve, water canal and dermis. Animals were allowed to heal for up to four weeks (2, 6, 12, 20, and 28 days post-injury) before sacrificed. Tissues were sectioned in a cryostat and changes in cellular and tissue elements during repair were evaluated using classical dyes, immmuohistochemistry and phalloidin labeling. In addition, the temporal and spatial distribution of cell proliferation in the animals was assayed using BrdU incorporation. We found that cellular events associated with wound healing in H. glaberrima correspond to those previously shown to occur during intestinal regeneration. These include: (1) an increase in the number of spherule-containing cells, (2) remodeling of the extracellular matrix, (3) formation of spindle-like structures that signal dedifferentiation of muscle cells in the area flanking the lesion site and (4) intense cellular division occurring mainly in the coelomic epithelium after the first week of regeneration. Our data indicate that H. glaberrima employs analogous cellular mechanisms during wound healing and organ regeneration. Thus, it is possible that regenerative limitations in some organisms are due

  19. Putative mechanism for apoptosis-inducing properties of crude saponin isolated from sea cucumber (Holothuria leucospilota) as an antioxidant compound

    PubMed Central

    Soltani, Mozhgan; Parivar, Kazem; Baharara, Javad; Kerachian, Mohammad Amin; Asili, Javad

    2015-01-01

    Objective(s): Marine organisms are known as a potential source of natural products, which contain bioactive substances with therapeutic properties. Sea cucumbers are prominent among marine organisms because of their dietary and therapeutic applications. In addition, they have capacity of synthesizing saponins molecules and other metabolites with therapeutic properties such as antitumor, antimicrobial, anti-inflammatory and antioxidant activities. The aim of this study was to evaluate the antioxidant and pro-apoptotic effects of sea cucumber saponins (SCS) isolated from Holothuria leucospilota species. Materials and Methods: Evaluation of antioxidant activity of SCS was carried out by DPPH (1, 1-diphenyl-2-picrylhydrazyl), ABTS (azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), power reducing and total antioxidant assays. The anti-proliferative effect was studied by MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Mechanisms leading to apoptosis were also evaluated by fluorescence microscopy, flow cytometry and real time PCR. Results: The results showed that the DPPH and ABTS activities increased in a dose dependent manner. The reducing capacity enhanced with increasing concentration of the saponin extract (0 to 2 mg/ml). The SCS exhibited moderate total antioxidant activity. Evaluation of anti-proliferative effect revealed that SCS with IC50 of about 6 μg/ml, can display a good cytotoxic activity in a dose dependent manner. Further apoptosis induction was confirmed by fluorescence microscopy and flow cytometry. Sea cucumber saponin was also found to exert a pro-apoptotic effect by increasing the expression of Bax and decreasing the expression of Bcl2. Conclusion: These results indicate that the SCS may act as a natural antioxidant and antitumor agent. PMID:25810893

  20. Discovery, structural characterization and functional analysis of alpha-2-macroglobulin, a novel immune-related molecule from Holothuria atra.

    PubMed

    Qian, Jing; Ren, Chunhua; Xia, Jianjun; Chen, Ting; Yu, Zonghe; Hu, Chaoqun

    2016-07-10

    The non-specific protease inhibitor alpha-2-macroglobulin (A2M) is a key macromolecular glycoprotein that involved in host immune defense against pathogens in vertebrates and invertebrates. However, no research regarding A2M has been developed in echinoderms to date. In this study, the full-length cDNA of A2M was cloned from the sea cucumber (Holothuria atra), which is a tropical species widely distributed along the coasts of the South China Sea and designated HaA2M. HaA2M possesses all three conserved functional domains of known A2M proteins, including the bait region domain, thioester domain and receptor-binding domain. Compared to fish and shrimp A2Ms, the histidine residue from the catalytical regions is well conserved in HaA2M. HaA2M mRNA was predominantly expressed in coelomocytes and, to a lesser extent, in the body wall, intestine and respiratory tree. A2M activity was detected in the coelomic fluids of H. atra. The mRNA expression and activity levels were investigated in the major immune tissues and coelomic fluids of H. atra after challenge with inactivated Vibrio alginolyticus or polyriboinosinic polyribocytidylic acid [Poly (I: C)]. RNA interference (RNAi)-mediated knockdown of HaA2M resulted in a significant reduction of HaA2M gene transcript level (86%). RNAi-mediated silencing of HaA2M gene significantly decreased the A2M activity (38%) and increased the number of viable bacteria (2.8-fold) in the coelomic fluids of H. atra infected by V. alginolyticus. Our study, as a whole, supplied the evidences for HaA2M as an immune-relevant molecule and it might have multiple functions in the innate immune system of H. atra. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Carbon amendment stimulates benthic nitrogen cycling during the bioremediation of particulate aquaculture waste

    NASA Astrophysics Data System (ADS)

    Robinson, Georgina; MacTavish, Thomas; Savage, Candida; Caldwell, Gary S.; Jones, Clifford L. W.; Probyn, Trevor; Eyre, Bradley D.; Stead, Selina M.

    2018-03-01

    The treatment of organic wastes remains one of the key sustainability challenges facing the growing global aquaculture industry. Bioremediation systems based on coupled bioturbation-microbial processing offer a promising route for waste management. We present, for the first time, a combined biogeochemical-molecular analysis of the short-term performance of one such system that is designed to receive nitrogen-rich particulate aquaculture wastes. Using sea cucumbers (Holothuria scabra) as a model bioturbator we provide evidence that adjusting the waste C : N from 5 : 1 to 20 : 1 promoted a shift in nitrogen cycling pathways towards the dissimilatory nitrate reduction to ammonium (DNRA), resulting in net NH4+ efflux from the sediment. The carbon amended treatment exhibited an overall net N2 uptake, whereas the control receiving only aquaculture waste exhibited net N2 production, suggesting that carbon supplementation enhanced nitrogen fixation. The higher NH4+ efflux and N2 uptake was further supported by meta-genome predictions that indicate that organic-carbon addition stimulated DNRA over denitrification. These findings indicate that carbon addition may potentially result in greater retention of nitrogen within the system; however, longer-term trials are necessary to determine whether this nitrogen retention is translated into improved sea cucumber biomass yields. Whether this truly constitutes a remediation process is open for debate as there remains the risk that any increased nitrogen retention may be temporary, with any subsequent release potentially raising the eutrophication risk. Longer and larger-scale trials are required before this approach may be validated with the complexities of the in-system nitrogen cycle being fully understood.

  2. Selective toxicity of persian gulf sea cucumber holothuria parva on human chronic lymphocytic leukemia b lymphocytes by direct mitochondrial targeting.

    PubMed

    Salimi, Ahmad; Motallebi, Abbasali; Ayatollahi, Maryam; Seydi, Enayatollah; Mohseni, Ali Reza; Nazemi, Melika; Pourahmad, Jalal

    2017-04-01

    Natural products isolated from marine environment are well known for their pharmacodynamic potential in diversity of disease treatments such as cancer or inflammatory conditions. Sea cucumbers are one of the marine animals of the phylum Echinoderm. Many studies have shown that the sea cucumber contains antioxidants and anti-cancer compounds. Chronic lymphocytic leukemia (CLL) is a disease characterized by the relentless accumulation of CD5 + B lymphocytes. CLL is the most common leukemia in adults, about 25-30% of all leukemias. In this study B lymphocytes and their mitochondria (cancerous and non-cancerous) were obtained from peripheral blood of human subjects and B lymphocyte cytotoxicity assay, and caspase 3 activation along with mitochondrial upstream events of apoptosis signaling including reactive oxygen species (ROS) production, collapse of mitochondrial membrane potential (MMP) and mitochondrial swelling were determined following the addition of Holothuria parva extract to both cancerous and non-cancerous B lymphocytes and their mitochondria. Our in vitro finding showed that mitochondrial ROS formation, MMP collapse, and mitochondrial swelling and cytochrome c release were significantly (P < 0.05) increased after addition of different concentrations of H. parva only in cancerous BUT NOT normal non-cancerous mitochondria. Consistently, different concentrations of H. parva significantly (P < 0.05) increased cytotoxicity and caspase 3 activation only in cancerous BUT NOT normal non-cancerous B lymphocytes. These results showed that H. parva methanolic extract has a selective mitochondria mediated apoptotic effect on chronic lymphocytic leukemia B lymphocytes hence may be promising in the future anticancer drug development for treatment of CLL. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1158-1169, 2017. © 2016 Wiley Periodicals, Inc.

  3. Fucosylated chondroitin sulfates from the body wall of the sea cucumber Holothuria forskali: conformation, selectin binding, and biological activity.

    PubMed

    Panagos, Charalampos G; Thomson, Derek S; Moss, Claire; Hughes, Adam D; Kelly, Maeve S; Liu, Yan; Chai, Wengang; Venkatasamy, Radhakrishnan; Spina, Domenico; Page, Clive P; Hogwood, John; Woods, Robert J; Mulloy, Barbara; Bavington, Charlie D; Uhrín, Dušan

    2014-10-10

    Fucosylated chondroitin sulfate (fCS) extracted from the sea cucumber Holothuria forskali is composed of the following repeating trisaccharide unit: → 3)GalNAcβ4,6S(1 → 4) [FucαX(1 → 3)]GlcAβ(1 →, where X stands for different sulfation patterns of fucose (X = 3,4S (46%), 2,4S (39%), and 4S (15%)). As revealed by NMR and molecular dynamics simulations, the fCS repeating unit adopts a conformation similar to that of the Le(x) blood group determinant, bringing several sulfate groups into close proximity and creating large negative patches distributed along the helical skeleton of the CS backbone. This may explain the high affinity of fCS oligosaccharides for L- and P-selectins as determined by microarray binding of fCS oligosaccharides prepared by Cu(2+)-catalyzed Fenton-type and photochemical depolymerization. No binding to E-selectin was observed. fCS poly- and oligosaccharides display low cytotoxicity in vitro, inhibit human neutrophil elastase activity, and inhibit the migration of neutrophils through an endothelial cell layer in vitro. Although the polysaccharide showed some anti-coagulant activity, small oligosaccharide fCS fragments had much reduced anticoagulant properties, with activity mainly via heparin cofactor II. The fCS polysaccharides showed prekallikrein activation comparable with dextran sulfate, whereas the fCS oligosaccharides caused almost no effect. The H. forskali fCS oligosaccharides were also tested in a mouse peritoneal inflammation model, where they caused a reduction in neutrophil infiltration. Overall, the data presented support the action of fCS as an inhibitor of selectin interactions, which play vital roles in inflammation and metastasis progression. Future studies of fCS-selectin interaction using fCS fragments or their mimetics may open new avenues for therapeutic intervention. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

  4. Leishmanicidal evaluation of extracts from native plants of the Yucatan peninsula.

    PubMed

    Peraza-Sánchez, S R; Cen-Pacheco, F; Noh-Chimal, A; May-Pat, F; Simá-Polanco, P; Dumonteil, E; García-Miss, M R; Mut-Martín, M

    2007-06-01

    Methanol extracts were prepared from different parts of 18 plants collected in the Yucatan peninsula and evaluated in an in vitro bioassay for leishmanicidal activity against Leishmania mexicana promastigotes. The ten most potent plant extracts (IC(50)<50 microg/ml) were Aphelandra scabra leaves, Byrsonima bucidaefolia bark, Byrsonima crassifolia bark, Clusia flava leaves, Cupania dentata bark, Diphysa carthagenensis leaves, Dorstenia contrajerva whole plant, Milleria quinqueflora roots, Tridax procumbens whole plant, and Vitex gaumeri bark.

  5. The effects of body temperature and mass on the postprandial metabolic responses of the African egg-eating snakes Dasypeltis scabra and Dasypeltis inornata.

    PubMed

    Greene, Sara; McConnachie, Suzanne; Secor, Stephen; Perrin, Mike

    2013-06-01

    African egg-eating snakes (Dasypeltis) feed only on freshly laid bird eggs which they perforate within their esophagus before swallowing the liquid contents and regurgitating the empty shell. Compared to a snake's typical intact meal, the liquid diet of Dasypeltis would expectedly generate a more moderate postprandial metabolic response and specific dynamic action (SDA). Free-ranging Dasypeltis feed over a range of ambient temperatures and thereby experience predicted temperature-dependent shifts in the duration and magnitude of their postprandial metabolic response. Such shifts would undoubtedly be shared among different species and age classes of Dasypeltis. To examine these expectations, we measured pre- and postprandial metabolic rates of adult Dasypeltis inornata and adult and neonate Dasypeltis scabra in response to liquid egg meals weighing 20% of snake body mass at 20, 25, 27, 30, and 32 °C. With an increase in body temperature, postprandial metabolic profiles of neonate and adult snakes became narrower and shorter in duration. Specific dynamic action varied among temperature treatments, increasing from 20 to 32 °C. Standard metabolic rate, postprandial peak metabolic rate, and SDA scaled with mass exponents that typically did not differ from 1.0. As expected, Dasypeltis digesting a liquid egg diet experienced a more modest postprandial response and SDA, expending on average only 10.6% of the meal's energy on the breakdown, absorption, and assimilation of the egg meal, whereas other colubrids consuming intact rodent or fish meals expend on average 16.3% of the meal's energy on digestion and assimilation. Actively foraging and feeding throughout the avian egg laying season enable Dasypeltis to survive when eggs are not available. The adaptive suite of traits that enable Dasypeltis to consume eggs of large relative size and ingest only the liquid contents may also be joined by physiological adaptations specific to their liquid diet and extended bouts of

  6. First echinoderm trehalase from a tropical sea cucumber (Holothuria leucospilota): Molecular cloning and mRNA expression in different tissues, embryonic and larval stages, and under a starvation challenge.

    PubMed

    Huo, Da; Jiang, Xiao; Wu, Xiaofen; Ren, Chunhua; Yu, Zonghe; Liu, Jinshang; Li, Hongmei; Ruan, Yao; Wen, Jin; Chen, Ting; Hu, Chaoqun

    2018-04-29

    Trehalases are a group of enzymes that catalyse the conversion of trehalose to glucose, and they are observed in most organisms. In this study, the first echinoderm trehalase, designated Hl-Tre, was identified from a tropical sea cucumber, Holothuria leucospilota. The full-length cDNA of H. leucospilota trehalase (Hl-Tre) is 2461 bp in length with an open reading frame (ORF) of 1788 bp that encodes a 595-amino-acid protein with a deduced molecular weight of 67.95 KDa. The Hl-Tre protein contains a signal peptide at the N-terminal and a functional trehalase domain, which includes the signature motifs 1 and 2. The mRNA expression of Hl-Tre was ubiquitously detected in all selected tissues, with the highest level being detected in the intestine. By in situ hybridization (ISH), the positive Hl-Tre signals were observed in the brush borders of the intestinal mucosa. In embryonic and larval stages, the transcript levels of Hl-Tre decreased during embryonic development and increased after the pentactula stage. After a challenge of starvation, the intestinal Hl-Tre mRNA levels were observed to be first decreased and partially recovered thereafter. Overall, our study provided the first evidence for trehalase in echinoderms and showed that this enzyme was potentially linked to a trehalose metabolic pathway in sea cucumbers. Copyright © 2017. Published by Elsevier B.V.

  7. Assessment of total and organic vanadium levels and their bioaccumulation in edible sea cucumbers: tissues distribution, inter-species-specific, locational differences and seasonal variations.

    PubMed

    Liu, Yanjun; Zhou, Qingxin; Xu, Jie; Xue, Yong; Liu, Xiaofang; Wang, Jingfeng; Xue, Changhu

    2016-02-01

    The objective of this study is to investigate the levels, inter-species-specific, locational differences and seasonal variations of vanadium in sea cucumbers and to validate further several potential factors controlling the distribution of metals in sea cucumbers. Vanadium levels were evaluated in samples of edible sea cucumbers and were demonstrated exhibit differences in different seasons, species and sampling sites. High vanadium concentrations were measured in the sea cucumbers, and all of the vanadium detected was in an organic form. Mean vanadium concentrations were considerably higher in the blood (sea cucumber) than in the other studied tissues. The highest concentration of vanadium (2.56 μg g(-1)), as well as a higher degree of organic vanadium (85.5 %), was observed in the Holothuria scabra samples compared with all other samples. Vanadium levels in Apostichopus japonicus from Bohai Bay and Yellow Sea have marked seasonal variations. Average values of 1.09 μg g(-1) of total vanadium and 0.79 μg g(-1) of organic vanadium were obtained in various species of sea cucumbers. Significant positive correlations between vanadium in the seawater and V org in the sea cucumber (r = 81.67 %, p = 0.00), as well as between vanadium in the sediment and V org in the sea cucumber (r = 77.98 %, p = 0.00), were observed. Vanadium concentrations depend on the seasons (salinity, temperature), species, sampling sites and seawater environment (seawater, sediment). Given the adverse toxicological effects of inorganic vanadium and positive roles in controlling the development of diabetes in humans, a regular monitoring programme of vanadium content in edible sea cucumbers can be recommended.

  8. Heat-shock protein 70 (Hsp70) expression in four limpets of the genus Lottia: interspecific variation in constitutive and inducible synthesis correlates with in situ exposure to heat stress.

    PubMed

    Dong, Yunwei; Miller, Luke P; Sanders, Jon G; Somero, George N

    2008-10-01

    Limpets of the genus Lottia occupy a broad vertical distribution on wave-exposed rocky shores, a range that encompasses gradients in the frequency and severity of thermal and desiccation stress brought on by aerial emersion. Using western blot analysis of levels of heat-shock protein 70 (Hsp70), we examined the heat-shock responses of four Lottia congeners: Lottia scabra and L. austrodigitalis, which occur in the high-intertidal zone, and L. pelta and L. scutum, which are restricted to the low- and mid-intertidal zones. Our results suggest distinct strategies of Hsp70 expression in limpets occupying different heights and orientations in the rocky intertidal zone. In freshly field-collected animals and in specimens acclimated at ambient temperature ( approximately 14 degrees C) for 14 days, the two high-intertidal species had higher constitutive levels of Hsp70 than the low- and mid-intertidal species. During aerial exposure to high temperatures, the two low-shore species and L. austrodigitalis exhibited an onset of Hsp70 expression at 28 degrees C; no induction of Hsp70 occurred in L. scabra. Our findings suggest that high-intertidal congeners of Lottia employ a "preparative defense" strategy involving maintenance of high constitutive levels of Hsp70 in their cells as a mechanism for protection against periods of extreme and unpredictable heat stress.

  9. Long Non-Coding RNAs (lncRNAs) of Sea Cucumber: Large-Scale Prediction, Expression Profiling, Non-Coding Network Construction, and lncRNA-microRNA-Gene Interaction Analysis of lncRNAs in Apostichopus japonicus and Holothuria glaberrima During LPS Challenge and Radial Organ Complex Regeneration.

    PubMed

    Mu, Chuang; Wang, Ruijia; Li, Tianqi; Li, Yuqiang; Tian, Meilin; Jiao, Wenqian; Huang, Xiaoting; Zhang, Lingling; Hu, Xiaoli; Wang, Shi; Bao, Zhenmin

    2016-08-01

    Long non-coding RNA (lncRNA) structurally resembles mRNA but cannot be translated into protein. Although the systematic identification and characterization of lncRNAs have been increasingly reported in model species, information concerning non-model species is still lacking. Here, we report the first systematic identification and characterization of lncRNAs in two sea cucumber species: (1) Apostichopus japonicus during lipopolysaccharide (LPS) challenge and in heathy tissues and (2) Holothuria glaberrima during radial organ complex regeneration, using RNA-seq datasets and bioinformatics analysis. We identified A. japonicus and H. glaberrima lncRNAs that were differentially expressed during LPS challenge and radial organ complex regeneration, respectively. Notably, the predicted lncRNA-microRNA-gene trinities revealed that, in addition to targeting protein-coding transcripts, miRNAs might also target lncRNAs, thereby participating in a potential novel layer of regulatory interactions among non-coding RNA classes in echinoderms. Furthermore, the constructed coding-non-coding network implied the potential involvement of lncRNA-gene interactions during the regulation of several important genes (e.g., Toll-like receptor 1 [TLR1] and transglutaminase-1 [TGM1]) in response to LPS challenge and radial organ complex regeneration in sea cucumbers. Overall, this pioneer systematic identification, annotation, and characterization of lncRNAs in echinoderm pave the way for similar studies and future genetic, genomic, and evolutionary research in non-model species.

  10. Studies on the functional morphology and ecology of the atyid prawns of Dominica.

    PubMed

    Fryer, G

    1977-02-25

    Six species of atyid prawns, representing five genera, occur in streams on the West Indian island of Dominica (figures 1-6). The ecology and habits of each are described and the relation of features of gross morphology to ways of life noted. Xiphocaris elongata, the most primitive living atyid, is a lightly built prawn whose adult habits are related to life in quiet pools in streams. An agile species and an excellent swimmer, it picks up individual small food particles with specialized chelipeds (figures 18 and 19) that differ from those of all other atyids and manipulates them with mouthparts (figure 77) which, while highly complex, are more primitive than those described for any other member of the family. Atya innocous and A. scabra, representing perhaps the most specialized atyid genus, are very similar in gross morphology and are robustly built ambulatory species. A. innocous is common in a variety of situations: A. scabra is rare and has been found only in fast-flowing water. Both have chelipeds whose three distal segments are extremely specialized (figure 36) and whose propus and dactylus are armed with an exceedingly complex array of long, slender bristles. These can be used either as brushes for collecting finely particulate detritus (figures 58-60) or as filtering fans (figures 68 and 69) which, held passively in flowing water, extract suspended particles. The Atyidae is unique among the Malacostraca in having representatives that filter passively by means of the chelipeds. The bristles (figure 40) are extended (figure 49), not by muscles, of which there are none in the distal parts of the propus and none anywhere in the dactylus, but by hydraulic forces. The return of the bristles to rest is by means of a cuticular spring. Some of the bristles of A. innocous are armed distally with minute denticles (figures 41 and 42) that facilitate scraping and sweeping: no such are present in A. scabra. The difference is related to the relative importance of scraping

  11. Copper tolerance in clones of Agrostis gigantea from a mine waste site

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hogan, G.D.; Courtin, G.M.; Rauser, W.E.

    1977-04-15

    A mine waste site from Sudbury, Ontario, contaminated with heavy metals is described. The dominant vegetative cover was formed by two grasses: Agrostis gigantea Roth. and Agrostis scabra Willd. Testing of 10 clones of A. gigantea from the roast bed and an adjoining area for copper tolerance showed that two clones collected from the roast bed were tolerant to increased copper levels. Copper tolerance was found in clones growing on soils with high copper contents and low pHs. The combination of high copper content and low pH brought about a high level of extractable copper within the soil. Soils withmore » equally high copper levels but higher pHs and therefore low extractable-copper levels did not support copper-tolerant clones.« less

  12. Summer weeds as hosts for Frankliniella occidentalis and Frankliniella fusca (Thysanoptera: Thripidae) and as reservoirs for tomato spotted wilt Tospovirus in North Carolina.

    PubMed

    Kahn, Noah D; Walgenbach, J F; Kennedy, G G

    2005-12-01

    In North Carolina, Tomato spotted wilt tospovirus (family Bunyaviridae, genus Tospovirus, TSWV) is vectored primarily by the tobacco thrips, Frankliniella fusca (Hinds), and the western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae). TSWV overwinters in winter annual weeds from which it is spread to susceptible crops in spring. Because most susceptible crops are destroyed after harvest before winter weeds emerge in the fall, infected summer weeds are thought to be the principal source for spread of TSWV to winter annual weeds in fall. A survey of summer weeds associated with TSWV-susceptible crops in the coastal plain of North Carolina conducted between May and October revealed that relatively few species were commonly infected with TSWV and supported populations of F. fusca or F. occidentalis. F. occidentalis made up > 75% of vector species collected from 15 summer weed species during 2002. The number of F. occidentalis and F. fusca immatures collected from plant samples varied significantly among plant species. Ipomoea purpurea (L.) Roth, Mollugo verticillata L., Cassia obtusifolia L., and Amaranthus palmeri S. Wats supported the largest numbers of immature F. occidentalis. Richardia scabra L., M. verticillata, and Ipomoea hederacea (L.) supported the largest numbers of F. fusca immatures. TSWV was present at 16 of 17 locations, and naturally occurring infections were found in 14 of 29 weed species tested. Five of the TSWV-infected species have not previously been reported as hosts of TSWV (A. palmeri, Solidago altissima L., Ipomoea lacunosa L., I. purpurea, and Phytolacca americana L.). Estimated rates of infection were highest in I. purpurea (6.8%), M. verticillata (5.3%), and I. hederacea (1.9%). When both the incidence of infection by TSWV and the populations of F. occidentalis and F. fusca associated with each weed species are considered, the following summer weed species have the potential to act as significant sources for

  13. Identification and characterization of an expansin gene AsEXP1 associated with heat tolerance in C3 Agrostis grass species.

    PubMed

    Xu, Jichen; Tian, Jiang; Belanger, Faith C; Huang, Bingru

    2007-01-01

    Plant tolerance of heat stress involves various changes at physiological and molecular levels. The objective of this study was to examine the expression of a gene encoding expansin protein in relation to heat tolerance in two C(3) grass species and genotypes differing in heat tolerance. Heat-tolerant, thermal Agrostis scabra, adapted to high temperatures in geothermal areas in Yellowstone National Park, was subjected to 20 degrees C (control) or 40 degrees C (heat stress) for 7 d in a growth chamber. Differential display analysis identified that a gene, AsEXP1, encoding an expansin protein, was strongly up-regulated in leaves exposed to heat stress in thermal A. scabra. Virtual northern hybridization and RT-PCR confirmed that AsEXP1 was a heat-inducible gene in leaves. The expression of AsEXP1 was induced at 1 h of plant exposure to heat stress and reached the highest level of expression at 4 h of treatment. A 1.3 kb full-length cDNA of AsEXP1 was isolated, which encodes a 251 amino acid protein. Two ecotypes of thermal A. scabra and 10 genotypes of Agrostis stolonifera (creeping bentgrass), a widely used turfgrass species in cool climatic regions, varying in the level of heat tolerance, were exposed to 40 degrees C for 7 d to examine the level of AsEXP1 expression in relation to heat tolerance. Genetic variation in heat tolerance was evaluated by measuring cell membrane stability, photochemical efficiency, and leaf growth. RT-PCR analysis revealed that the level of AsEXP1 in different genotypes was positively correlated with the level of heat tolerance in both grass species. The results first identified a heat-related expansin gene in grass species and suggest that AsEXP1 may be useful as a molecular marker to select for heat-tolerant grass germplasm.

  14. New Coleoptera records from New Brunswick, Canada: Trogossitidae, Cleridae, and Melyridae, with an addition to the fauna of Nova Scotia

    PubMed Central

    Webster, Reginald P.; Sweeney, Jon D.; DeMerchant, Ian

    2012-01-01

    Abstract Grynocharis quadrilineata (Melsheimer) and Tenebroides corticalis (Melsheimer) of the family Trogossitidae are newly recorded for New Brunswick, Canada. Additional records of the recently reported Calitys scabra (Thunberg)and Ostoma fraterna (Randall) are presented for the province. The record of Ostoma fraterna is the first recent record of this species from New Brunswick. Additional New Brunswick records of the thaneroclerine, Zenodosus sanguineus (Say), are given, indicting that this species is common and widespread in the province. One species of Cleridae, Cymatodera bicolor (Say),is newly reported from New Brunswick, and the adventive Thanasimus formicarius Linnaeus is newly recorded from Nova Scotia and the Maritime provinces. Attalus morulus (LeConte) and Dolichosoma foveicolle (Kirby), family Melyridae, are reported for the first time for New Brunswick and the Maritime provinces. Collection, habitat data, and distribution maps are presented for these species. PMID:22539891

  15. Qualitative and Quantitative Saponin Contents in Five Sea Cucumbers from the Indian Ocean

    PubMed Central

    Van Dyck, Séverine; Gerbaux, Pascal; Flammang, Patrick

    2010-01-01

    To avoid predation, holothuroids produce feeding-deterrent molecules in their body wall and viscera, the so-called saponins. Five tropical sea cucumber species of the family Holothuriidae were investigated in order to study their saponin content in two different organs, the body wall and the Cuvierian tubules. Mass spectrometry techniques (MALDI- and ESI-MS) were used to detect and analyze saponins. The smallest number of saponins was observed in Holothuria atra, which contained a total of four congeners, followed by Holothuria leucospilota, Pearsonothuria graeffei and Actinopyga echinites with six, eight and ten congeners, respectively. Bohadschia subrubra revealed the highest saponin diversity (19 congeners). Saponin mixtures also varied between the two body compartments within a given animal. A semi-quantitative approach completed these results and showed that a high diversity of saponins is not particularly correlated to a high saponin concentration. Although the complexity of the saponin mixtures described makes the elucidation of their respective biological roles difficult, the comparisons between species and between body compartments give some clues about how these molecules may act as predator repellents. PMID:20161976

  16. [HPLC investigation of antioxidant components in Solidago herba].

    PubMed

    Apáti, Pál; Houghton, Peter J; Kéry, Agnes

    2004-01-01

    Representatives of Solidago species have been used in European phytotheraphy for centuries as a component of urological and antiphlogistical remedies. Solidago canadensis L. (Asteraceae) contains a wide range of active ingredients, such as flavonoids, saponins, hydroxycinnamates and mineral elements, which are responsible for its characteristic anti-inflammatory, spasmolytic and diuretic properties. Quality control of collected Solidaginis herba were performed according to the instructions of the X. German Pharmacopoea, while different LC-MS technologies were applied to evaluate the exact phenoloid composition. Three flavonol aglycons (quercetin, kaempferol and isorhamnetin) connected to several sugar components (glucose, rhamnose, galactose and rutinose), caffeoylquinic acid and a caffeoyl-shikimic acid glycoside were identified in the samples. Quercetin-3-O-beta-glucoside (isoquercitrin), quercetin-3-O-beta-galactoside (hyperoside), quercetin-3-O-beta-rhamnoside (quercitrin), quercetin-3-O-beta-rutinoside (rutin), kaempferol-3-O-beta-rhamnoside (afzelin), kaempferol-3-O-beta-rutinoside (nicotiflorin), caffeoil-quinic acid (chlorogenic acid) were identified in sample "A", while the presence of quercetin, quercetin-3-O-beta-glucoside (isoquercitrin), quercetin-3-/6"-O-acetyl-/-beta-glucopiranoside, quercetin-3-O-beta-rutinoside (rutin), kaempferol, kaempferol-3-O-beta-glucoside (astragalin), kaempferol-3-/6"-O-acetyl-/-beta-glucopiranoside, isorhamnetin, isorhamnetin-3-/6"-O-acetyl-/-beta-glucopiranoside, isorhamnetin-3-O-beta-rutinoside (narcissin), caffeoil-quinic acid (chlorogenic acid), caffeoil-shikimic acid-glucoside (dattelic acid-glucoside) were confirmed in sample "B". According to the occurrence of acetyl-glycosides and the diversity of sugar component of flavonoid glycosides Solidaginis herba samples chemotaxonomically were classified into different varieties. Incidence of acetyl-glycosidic flavonoids and absence of flavonoid galactosides and rhamnosides

  17. [The echinoderms (Echinodermata) from El Salvador].

    PubMed

    Enrique Barraza, José; Roberto Hasbún, Carlos

    2005-12-01

    A list of echinoderms from El Salvador (tropical eastern Pacific) is presented. The results were obtained from field surveys (between the years 2000 and 2004), the scarce literature sources, and Internet information. A total of 37 species and six genera are reported. The most abundant echinoderms in rocky shores were: Phataria unifascialis, Echinometra vanbrunti, Holothuria kefersteini, as well as Astropecten armatus in soft bottoms.

  18. Rio Bauta Abajo, Orocovis, Puerto Rico Monitoring: Citizen Science in Action

    NASA Astrophysics Data System (ADS)

    Rivera, L. D.; Rodriguez, C.

    2015-12-01

    "Conoce tu rio" (Learn about your river) is a Citizen Science-"Para La Naturaleza" Project with the purpose of providing citizens effective monitoring methods for water quality and macro invertebrates. Through the monthly monitoring of Rio Grande de Manatí watershed in Puerto Rico, volunteers gained the knowledge and skills necessary to conduct the same methodology in other rivers of Puerto Rico. This is the case of the Rio Bauta Abajo-Orocovis group, initiated by a volunteer of "Conoce tu Rio". The new group has the objective of monitoring the water quality, shrimps and other macro invertebrate diversity and abundance in a stream reach of Rio Bauta Abajo, Orocovis, Puerto Rico. The group is also part of the EPA Equipment Loan Program for Pathogen and Water Quality Monitoring. Shrimps and macro invertebrates are captured using nets. In this ongoing project, preliminary results show that 5 of the 17 shrimp species of Puerto Rico and the freshwater crab have been captured in the monitoring point of Rio Bauta Abajo. The most abundant species captured was Atya scabra representing 70% of the abundance. The second most abundant species was Xiphocaris elongata representing 20% of the abundance. We also found a large number of gravid females of two shrimp species. Sixty percent of captured Atya scabra were gravid while 100% of the captured Xiphocaris elongata were gravid. Water quality is within the standard except for phosphorous and nitrogen. The most abundant species inhabit different habitats in the river. Atya scabra is found in the riffles and Xiphocaris elongata is found in the stream borders and or pools. The high number of gravid females suggests that the dry season is the reproductive season for this two species. The high concentration of phosphorous and nitrogen suggest that in the area there area either crops of animals facilities whose runoff is getting to the river. This monitoring group expects to continue motivating citizens to monitor the water quality in

  19. 76 FR 74812 - Agency Information Collection Activities: Proposed Collection; Comment Request New Collection...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-01

    ... Collection Activities: Proposed Collection; Comment Request New Collection; 2012 Census of Adult Probation... Adult Probation Supervising Agencies is now a standalone collection. This collection's scope is narrower and only includes adult probation agencies and the adult probationers supervised by the agencies. The...

  20. Workforce Competitiveness Collection. "LINCS" Resource Collection News

    ERIC Educational Resources Information Center

    Literacy Information and Communication System, 2011

    2011-01-01

    This edition of "'LINCS' Resource Collection News" features the Workforce Competitiveness Collection, covering the topics of workforce education, English language acquisition, and technology. Each month Collections News features one of the three "LINCS" (Literacy Information and Communication System) Resource Collections--Basic…

  1. Chemical composition and nutritional quality of sea cucumbers.

    PubMed

    Wen, Jing; Hu, Chaoqun; Fan, Sigang

    2010-11-01

    The dried form of sea cucumbers has been a seafood and medicinal cure for Asians over many centuries. In this study the chemical composition and nutritional quality of eight common sea cucumbers (Stichopus herrmanni, Thelenota ananas, Thelenota anax, Holothuria fuscogilva, Holothuria fuscopunctata, Actinopyga mauritiana, Actinopyga caerulea and Bohadschia argus) were determined. All species except T. anax and A. caerulea had higher protein and lower fat levels. A. mauritiana and B. argus had less ash content. Glycine was the dominant amino acid found in all species, and content ranged from 126 to 216 mg g(-1) of crude protein. All species exhibited low lysine:arginine ratio and higher essential amino acid scores were obtained by threonine and phenylalanine + tyrosine. A. mauritiana had proportionally less saturated fatty acids (31.23%), and more monounsaturated fatty acids (45.64%) and polyunsaturated fatty acids (PUFA, 23.13%) than other species. Arachidonic acid (C20:4n-6) was the major PUFA in all species. T. ananas, A. mauritiana and A. caerulea contained more n-3 PUFA. The n-3/n-6 ratios of eight sea cucumbers species ranged from 0.25 to 0.61. Sea cucumbers are a seafood with high protein and low fat levels. The amino acid contents were similar but fatty acid profiles were different among species. The comparison showed that T. ananas, A. mauritiana and B. argus possessed higher nutritional values than other sea cucumber species. 2010 Society of Chemical Industry

  2. 78 FR 2693 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Collection of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-14

    ... DEPARTMENT OF JUSTICE Drug Enforcement Administration [OMB Number 1117-0034] Agency Information Collection Activities; Proposed Collection; Comments Requested: Collection of Laboratory Analysis Data on... information collection request to the Office of Management and Budget (OMB) for review and approval in...

  3. Program Management Collection. "LINCS" Resource Collection News

    ERIC Educational Resources Information Center

    Literacy Information and Communication System, 2011

    2011-01-01

    This edition of "'LINCS' Resource Collection News" features the Program Management Collection, which covers the topics of Assessment, Learning Disabilities, and Program Improvement. Each month Collections News features one of the three "LINCS" (Literacy Information and Communication System) Resource Collections--Basic Skills, Program Management,…

  4. [Location of semen collection and semen quality: clinic-collected versus home-collected samples].

    PubMed

    Wang, Wei; Zhong, Zhi-min; Su, Ning; Peng, Ya-ya; Huang, Ting-ting

    2014-11-01

    To investigate the differences in semen quality between samples collected by masturbation in the clinic and at home. Based on the WHO guidelines, we analyzed the ejaculates collected by masturbation in the clinic and at home from 342 men under infertility assessment and measured the contents of such biochemical markers in the seminal plasma as neutral α-glucosidase, zinc, and fructose. According to the location of semen collection, we divided the samples into two groups, clinic-collected and home-collected, and analyzed the differences in the semen parameters between the two groups with the SPSS 16.0 software. Compared with the clinic-collected semen, the home-collected samples had significantly higher mean values in semen volume (4.0 vs 4.9%), sperm concentration (41 vs 64 x 10(6)/ml), total sperm count (175 vs 270 x 10(6) per ejaculate), progressive sperm motility (40 vs 52%), total count of progressively motile sperm (82 vs 135 x 10(6) per ejaculate) (all P <0.05). No significant differences were found between the two groups in normal sperm morphology (4.0 vs 5.0%) and the contents of neutral α-glucosidase (26 vs 24 mU per ejaculate), zinc (8.0 vs 8.0 μmol per ejaculate), and fructose (62 vs 60 μmol per ejaculate) (all P >0.05). Abnormal sperm concentration (<20 x 10(6)/ml) was observed in significantly fewer of the home-collected samples than the clinic-collected ones (18% [62/342] vs 30% [103/342], P<0.05), and so was abnormal progressive sperm motility (<32%) (64% [219/342] vs 75% [256/342], P<0.05). Our findings show that semen samples collected by masturbation at home has a higher quality than those collected in the clinic. So the location of semen collection should be taken into consideration in infertility investigation.

  5. Collection Directions: The Evolution of Library Collections and Collecting

    ERIC Educational Resources Information Center

    Dempsey, Lorcan; Malpas, Constance; Lavoie, Brian

    2014-01-01

    This article takes a broad view of the evolution of collecting behaviors in a network environment and suggests some future directions based on various simple models. The authors look at the changing dynamics of print collections, at the greater engagement with research and learning behaviors, and at trends in scholarly communication. The goal is…

  6. 78 FR 64012 - Agency Information Collection Activities; Proposed Collection; Comments Requested; New Collection...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-25

    ... DEPARTMENT OF JUSTICE [OMB Number 1121--NEW] Agency Information Collection Activities; Proposed Collection; Comments Requested; New Collection: 2013 Census of Federal, State, and Local Law Enforcement Agencies ACTION: 60-day notice. The Department of Justice (DOJ), Office of Justice Programs, Bureau of...

  7. Collecting apparatus

    DOEpatents

    Duncan, Charles P.

    1983-01-01

    An improved collecting apparatus for small aquatic or airborne organisms such as plankton, larval fish, insects, etc. The improvement constitutes an apertured removal container within which is retained a collecting bag, and which is secured at the apex of a conical collecting net. Such collectors are towed behind a vessel or vehicle with the open end of the conical net facing forward for trapping the aquatic or airborne organisms within the collecting bag, while allowing the water or air to pass through the apertures in the container. The container is readily removable from the collecting net whereby the collecting bag can be quickly removed and replaced for further sample collection. The collecting bag is provided with means for preventing the bag from being pulled into the container by the water or air flowing therethrough.

  8. Natural Terpenoids from Ambrosia Species Are Active In Vitro and In Vivo against Human Pathogenic Trypanosomatids

    PubMed Central

    Frank, Fernanda M.; Laurella, Laura C.; Muschietti, Liliana V.; Catalán, Cesar A.; Martino, Virginia S.; Malchiodi, Emilio L.

    2013-01-01

    Among the natural compounds, terpenoids play an important role in the drug discovery process for tropical diseases. The aim of the present work was to isolate antiprotozoal compounds from Ambrosia elatior and A. scabra. The sesquiterpene lactone (STL) cumanin was isolated from A. elatior whereas two other STLs, psilostachyin and cordilin, and one sterol glycoside, daucosterol, were isolated from A. scabra. Cumanin and cordilin were active against Trypanosoma cruzi epimastigotes showing 50% inhibition concentrations (IC50) values of 12 µM and 26 µM, respectively. Moreover, these compounds are active against bloodstrean trypomastigotes, regardless of the T. cruzi strain tested. Psilostachyin and cumanin were also active against amastigote forms with IC50 values of 21 µM and 8 µM, respectively. By contrast, daucosterol showed moderate activity on epimastigotes and trypomastigotes and was inactive against amastigote forms. We also found that cumanin and psilostachyin exhibited an additive effect in their trypanocidal activity when these two drugs were tested together. Cumanin has leishmanicidal activity with growth inhibition values greater than 80% at a concentration of 5 µg/ml (19 µM), against both L. braziliensis and L. amazonensis promastigotes. In an in vivo model of T. cruzi infection, cumanin was more active than benznidazole, producing an 8-fold reduction in parasitemia levels during the acute phase of the infection compared with the control group, and more importantly, a reduction in mortality with 66% of the animals surviving, in comparison with 100% mortality in the control group. Cumanin also showed nontoxic effects at the doses assayed in vivo, as determined using markers of hepatic damage. PMID:24130916

  9. 77 FR 46527 - Agency Information Collection Activities; Proposed Collection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-03

    ... MERIT SYSTEMS PROTECTION BOARD Agency Information Collection Activities; Proposed Collection... extension of an Information Collection Request (ICR) to the Office of Management and Budget (OMB) for review and approval. This ICR describes the nature of the information collection and its estimated burden and...

  10. 77 FR 32118 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-31

    ... this evaluation, qualitative and quantitative data will be collected and analyzed to answer research... minimize the information collection burden. 1. Type of Information Collection Request: New collection.... For all other issues call 410-786- 1326.) 2. Type of Information Collection Request: New collection...

  11. 75 FR 1084 - Agency Information Collection Activities: New Collection, Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-08

    ... collection under review: New collection; InfraGard Knowledge/Skills/Abilities Profile questionnaire. The... be submitting the following information collection request to the Office of Management and Budget... information collection: New collection. (2) The title of the form/collection: InfraGard Knowledge/Skills...

  12. Structure of gastropod communities at mangrove ecosystem in Lubuk Kertang village, West Berandan District, Langkat Regency, North Sumatera Province

    NASA Astrophysics Data System (ADS)

    Manullang, T.; Bakti, D.; Leidonald, R.

    2018-02-01

    Gastropod was one of the class from mollusca in mangrove ecosystem. Lubuk Kertang Village’s mangrove forest was been converted into tourist areas, agricultural land and settlements. The purpose of this study was to analyze the structure of gastropods at Avicennia lanata, Rhizophora apiculata and Sonneratia alba. This research was conducted at Lubuk Kertang Village in February-March 2017. Gastropod were collected in 1 m × 1 m transect in mangrove. Examples of biota were taken by using shovel, then the biota was inserted into a plastic bag sample, wrote date of sampling and identified. The results showed there were 15 species Gastropods, namely Achatina fulica, Cerithidea alata, Cerithidea cingulata, Cerithidea obtusa, Chicoreus capucinus, Cymatium pileare, Ellobium aurimisdae, Ellobium aurisjudae, Littoraria melanostoma, Littoraria scabra, Murex tribulus, Nerita balteata, Nerita planospira, Pugilina cochlidium, Stramonita gradata, Telescopium telescopium and Terebralia sulcata. Diversity index ranged 1.702 to 2.165 was in medium category, Similarity index ranged 0.676 to 0.799 was in low category and Dominance index ranged 0.142 to 0.282 that categorized was low. The highest gastropod density was 31 individuals/m2 in the Sonneratia alba. The conclusion of the research is the existing mangrove ecosystem in Lubuk Kertang Village in a stable state.

  13. Collective enumeration.

    PubMed

    Bahrami, Bahador; Didino, Daniele; Frith, Chris; Butterworth, Brian; Rees, Geraint

    2013-04-01

    Many joint decisions in everyday life (e.g., Which bar is less crowded?) depend on approximate enumeration, but very little is known about the psychological characteristics of counting together. Here we systematically investigated collective approximate enumeration. Pairs of participants made individual and collective enumeration judgments in a 2-alternative forced-choice task and when in disagreement, they negotiated joint decisions via verbal communication and received feedback about accuracy at the end of each trial. The results showed that two people could collectively count better than either one alone, but not as well as expected by previous models of collective sensory decision making in more basic perceptual domains (e.g., luminance contrast). Moreover, such collective enumeration benefited from prior, noninteractive practice showing that social learning of how to combine shared information about enumeration required substantial individual experience. Finally, the collective context had a positive but transient impact on an individual's enumeration sensitivity. This transient social influence may be explained as a motivational factor arising from the fact that members of a collective must take responsibility for their individual decisions and face the consequences of their judgments.

  14. 76 FR 72505 - Agency Information Collection Activity; Proposed Collection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-23

    ... DEPARTMENT OF THE TREASURY Internal Revenue Service Agency Information Collection Activity; Proposed Collection AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for... on proposed and/or continuing information collections, as required by the Paperwork Reduction Act of...

  15. Collection Directions: Some Reflections on the Future of Library Collections and Collecting

    ERIC Educational Resources Information Center

    Dempsey, Lorcan; Malpas, Constance; Lavoie, Brian

    2014-01-01

    This article takes a broad view of the evolution of collecting behaviors in a network environment and suggests some future directions based on various simple models. The authors look at the changing dynamics of print collections, at the greater engagement with research and learning behaviors, and at trends in scholarly communication. The goal is…

  16. Collections Conservation.

    ERIC Educational Resources Information Center

    DeCandido, Robert

    Collections conservation is an approach to the preservation treatment of books and book-like materials that is conceptualized and organized in terms of large groups of materials. This guide is intended to enable a library to evaluate its current collections conservation activities. The introduction describes collections conservation and gives…

  17. Biological effects of some natural and chemical compounds on the potato tuber moth, Phthorimaea operculella Zell. (Lepidoptera:Gelechiidae)

    PubMed Central

    Sharaby, Aziza; Abdel-Rahman, H.; Moawad, S.

    2009-01-01

    The olfactory reaction of larvae and moths was investigated towards 18 oils (6 natural oils and 12 commercial chemical oils). Some of these oils such as peppermint and camphor (natural oils) and eugenol and camphene (commercial oils) were repellent to both larvae and moths. Other oils such as strawberry and d-limonene were attractive to both larvae and moths. Some of the repellent oils were, therefore, tested for their effect on certain biological aspects of the insects. Eugenol and peppermint oils, each at the 0.01% conc., caused a significant depression in the fecundity of moth and decreased the percentage of egg hatchability. Eugenol oil was much more effective than peppermint oil at 1%. Dried (leaves, fruits or seeds) powder of 14 different plants species were tested in different concentrations with talcum powder (carrier material) against egg deposition. The results indicated that dried powders of Allium cepa, Curcuma longa, Colocasia antiqurum, Ocimum basilicum. Dodonaea viscose and Thuja orientalis played a highly significant role in reducing egg deposition. The most impressive effect was displayed by powders of D. viscose and A. cepa, which caused the highest depression in egg deposition as well as in the emerging offsprings. Ethanolic extracts of 11 plants indicated that extracts of Pithuranthos tortosus and Iphiona scabra caused the maximum inhibition of egg hatchability, followed by C. longa, Citrullus colocynthia and T. orientalis. Ethanolic extracts of Schinus terebenthiflius (leaves) and I. scabra caused the highest depression in the deposited eggs, as they played a remarkable role as ovipositor deterrents. The majority of the plant extracts at 1% conc. could protect potato tubers at different intervals according to the calculated tuber damage index as follows: Iphiopna > Pithuranthos > Curcuma > Schinus (fruits) Thuja > Schinus (leaves) > Dodonaea > Citrullus. PMID:23961036

  18. Biological effects of some natural and chemical compounds on the potato tuber moth, Phthorimaea operculella Zell. (Lepidoptera:Gelechiidae).

    PubMed

    Sharaby, Aziza; Abdel-Rahman, H; Moawad, S

    2009-07-01

    The olfactory reaction of larvae and moths was investigated towards 18 oils (6 natural oils and 12 commercial chemical oils). Some of these oils such as peppermint and camphor (natural oils) and eugenol and camphene (commercial oils) were repellent to both larvae and moths. Other oils such as strawberry and d-limonene were attractive to both larvae and moths. Some of the repellent oils were, therefore, tested for their effect on certain biological aspects of the insects. Eugenol and peppermint oils, each at the 0.01% conc., caused a significant depression in the fecundity of moth and decreased the percentage of egg hatchability. Eugenol oil was much more effective than peppermint oil at 1%. Dried (leaves, fruits or seeds) powder of 14 different plants species were tested in different concentrations with talcum powder (carrier material) against egg deposition. The results indicated that dried powders of Allium cepa, Curcuma longa, Colocasia antiqurum, Ocimum basilicum. Dodonaea viscose and Thuja orientalis played a highly significant role in reducing egg deposition. The most impressive effect was displayed by powders of D. viscose and A. cepa, which caused the highest depression in egg deposition as well as in the emerging offsprings. Ethanolic extracts of 11 plants indicated that extracts of Pithuranthos tortosus and Iphiona scabra caused the maximum inhibition of egg hatchability, followed by C. longa, Citrullus colocynthia and T. orientalis. Ethanolic extracts of Schinus terebenthiflius (leaves) and I. scabra caused the highest depression in the deposited eggs, as they played a remarkable role as ovipositor deterrents. The majority of the plant extracts at 1% conc. could protect potato tubers at different intervals according to the calculated tuber damage index as follows: Iphiopna > Pithuranthos > Curcuma > Schinus (fruits) Thuja > Schinus (leaves) > Dodonaea > Citrullus.

  19. 77 FR 47851 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-10

    ... Collection: Minimum Data Set for Medicaid Incentives for Prevention of Chronic Diseases Program Grantees; Use... collection, the MIPCD Minimum Data Set (MDS), is intended to collect data for program performance monitoring and evaluation. The MDS is a secondary data collection that assembles information already collected by...

  20. 75 FR 43989 - Agency Information Collection Activities; Proposed Collection; Comment Request; Sample Collection...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-27

    ... for Dogs Treated With SLENTROL AGENCY: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The... on the sample collection plan for dogs treated with the drug SLENTROL. DATES: Submit either... technology. Sample Collection Plan for Dogs Treated With SLENTROL--21 CFR 514.80 (OMB Control Number 0910-NEW...

  1. PLOS Collections: Article collections published by the Public Library of

    Science.gov Websites

    Collections Propose a Special Collection Finances for Special Collections Browse Biology & Life Sciences Advertise Media Inquiries Publications PLOS Biology PLOS Medicine PLOS Computational Biology PLOS Currents

  2. 77 FR 36010 - Agency Information Collection Activities; Agency Information Collection Activities; Proposed...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-15

    ... Collection; Comment Requested Deaths in Custody--Series of Collections From State-Level Law Enforcement... collection: Renewal of existing collection. (2) The title of the Form/Collection: Deaths in Custody Reporting... collection: Forms--Death Report on Inmates Under Jail Jurisdiction (CJ-9); Annual Summary on Inmates Under...

  3. 75 FR 45664 - Agency Information Collection Activities; Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-03

    ... DEPARTMENT OF JUSTICE Justice Management Division [OMB Number 1103-0018] Agency Information Collection Activities; Proposed Collection; Comments Requested ACTION: 30-Day Notice of Information Collection Under Review: Extension of Previously Approved Collection, Department of Justice Procurement...

  4. 78 FR 22889 - Agency Information Collection Activities; Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-17

    ... the data collection plans and draft instruments, email [email protected] or call the HRSA Reports... technology to minimize the information collection burden. Information Collection Request Title: Data... utilize technology and systems for the purpose of collecting, validating and verifying information...

  5. 78 FR 27241 - Agency Information Collection Activities; Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... the data collection plans and draft instruments, email [email protected] or call the HRSA Information... technology to minimize the information collection burden. Information Collection Request Title... instructions; to develop, acquire, install and utilize technology and systems for the purpose of collecting...

  6. 75 FR 81648 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-28

    ... of information collection under review: notification of change of mailing or premise address. The... technological collection techniques or other forms of information technology, e.g., permitting electronic...: Extension of a currently approved collection. (2) Title of the Form/Collection: Notification of Change of...

  7. 76 FR 24053 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-29

    ... that captures valid, reliable data on the nature and extent of intimate partner violence, sexual... Information Collection Activity; Comment Request, Proposed Project entitled ``Violence and Victimization... Collection: (1) Type of Information Collection: Survey. (2) The title of the Form/Collection: Violence and...

  8. 77 FR 30325 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Notification...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-22

    ...] Agency Information Collection Activities: Proposed Collection; Comments Requested: Notification of Change... through the use of appropriate automated, electronic, mechanical, or other technological collection... currently approved collection. (2) Title of the Form/Collection: Notification of Change of Mailing or...

  9. 78 FR 45275 - Agency Information Collection Activities; Proposed collection; Comments Requested: Notification...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-26

    ...] Agency Information Collection Activities; Proposed collection; Comments Requested: Notification of Change... technological collection techniques or other forms of information technology, e.g., permitting electronic... currently approved collection. (2) Title of the Form/Collection: Notification of Change of Mailing or...

  10. 76 FR 10391 - Agency Information Collection Activities: Proposed Collection; Comments Requested: ARCOS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    ... Collection Activities: Proposed Collection; Comments Requested: ARCOS Transaction Reporting; DEA Form 333... Enforcement Administration (DEA), will be submitting the following information collection request to the... approved collection. (2) Title of the Form/Collection: ARCOS Transaction Reporting--DEA Form 333. (3...

  11. 76 FR 59410 - Agency Information Collection Activities: Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-26

    ... Information Collection Activities: Proposed Collection: Comment Request In compliance with the requirement for.... To request more information on the proposed project or to obtain a copy of the data collection plans...) 443-1129. Comments are invited on: (a) The proposed collection of information for the proper...

  12. 75 FR 14630 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-26

    ... on a recurring basis. In the SCPS data collection program, felony defendants are tracked for up to 1 year with data collected on a variety of felony case processing characteristics. These include the... convicted, sentencing data are collected. The SCPS 2009 project also involves collecting aggregate...

  13. 75 FR 6707 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-10

    ... of Information Collection Under Review: Police Check Inquiry. The Department of Justice (DOJ), Bureau... Collection: Extension of a currently approved collection. (2) Title of the Form/Collection: Police Check... necessary to permit ATF to complete and/or initiate a police check inquiry consisting of criminal record...

  14. Performance of a New HPV Cervi-Collect Collection and Transportation Kit

    PubMed Central

    Chernesky, M.; Huang, S.; Jang, D.; Erickson, B.; Salituro, J.; Engel, H.; Gilchrist, J.; Neuscheler, P.; Mak, W. B.; Abravaya, K.

    2012-01-01

    Background. Liquid-based Pap (L-Pap) media are used for Pap and human papillomavirus (HPV) testing. Objectives. To compare RealTime High Risk (HR) HPV testing of a new collection kit (Cervi-Collect) and PreservCyt L-Pap specimens. To determine ease of use and safety of Cervi-Collect. Methods. L-Pap samples (n = 203) were tested with HC2 and RealTime HR HPV and Cervi-Collect with RealTime HR HPV. Discordant samples were genotyped. Results. L-Pap and Cervi-Collect specimens tested by RealTime HR HPV showed 93.1% agreement (Kappa 0.86). RealTime HR HPV and HC2 on L-Pap had 90.3% agreement (Kappa 0.80). RealTime HR HPV on Cervi-Collect and HC2 on L-Pap showed 88.2% agreement (Kappa 0.76). Sixteen of 21 samples which were HC2 negative and RealTime HR HPV positive on L-Pap or Cervi-Collect contained HR HPV genotypes. Eleven healthcare collectors were in strong agreement on a usability and safety questionnaire. Conclusion. Cervi-Collect samples were easy to collect and showed strong agreement with L-Pap samples tested with RealTime HR HPV or HC2. PMID:22174716

  15. New Invertebrate Vectors of Okadaic Acid from the North Atlantic Waters--Portugal (Azores and Madeira) and Morocco.

    PubMed

    Silva, Marisa; Rodriguez, Inés; Barreiro, Aldo; Kaufmann, Manfred; Isabel Neto, Ana; Hassouani, Meryem; Sabour, Brahim; Alfonso, Amparo; Botana, Luis M; Vasconcelos, Vitor

    2015-12-08

    Okadaic acid and its analogues are potent phosphatase inhibitors that cause Diarrheic Shellfish Poisoning (DSP) through the ingestion of contaminated shellfish by humans. This group of toxins is transmitted worldwide but the number of poisoning incidents has declined over the last 20 years due to legislation and monitoring programs that were implemented for bivalves. In the summer of 2012 and 2013, we collected a total of 101 samples of 22 different species that were made up of benthic and subtidal organisms such echinoderms, crustaceans, bivalves and gastropods from Madeira, São Miguel Island (Azores archipelago) and the northwestern coast of Morocco. The samples were analyzed by UPLC-MS/MS. Our main objective was to detect new vectors for these biotoxins. We can report nine new vectors for these toxins in the North Atlantic: Astropecten aranciacus, Arbacia lixula, Echinaster sepositus, Holothuria sanctori, Ophidiaster ophidianus, Onchidella celtica, Aplysia depilans, Patella spp., and Stramonita haemostoma. Differences in toxin contents among the species were found. Even though low concentrations were detected, the levels of toxins that were present, especially in edible species, indicate the importance of these types of studies. Routine monitoring should be extended to comprise a wider number of vectors other than for bivalves of okadaic acid and its analogues.

  16. New Invertebrate Vectors of Okadaic Acid from the North Atlantic Waters—Portugal (Azores and Madeira) and Morocco

    PubMed Central

    Silva, Marisa; Rodriguez, Inés; Barreiro, Aldo; Kaufmann, Manfred; Neto, Ana Isabel; Hassouani, Meryem; Sabour, Brahim; Alfonso, Amparo; Botana, Luis M.; Vasconcelos, Vitor

    2015-01-01

    Okadaic acid and its analogues are potent phosphatase inhibitors that cause Diarrheic Shellfish Poisoning (DSP) through the ingestion of contaminated shellfish by humans. This group of toxins is transmitted worldwide but the number of poisoning incidents has declined over the last 20 years due to legislation and monitoring programs that were implemented for bivalves. In the summer of 2012 and 2013, we collected a total of 101 samples of 22 different species that were made up of benthic and subtidal organisms such echinoderms, crustaceans, bivalves and gastropods from Madeira, São Miguel Island (Azores archipelago) and the northwestern coast of Morocco. The samples were analyzed by UPLC-MS/MS. Our main objective was to detect new vectors for these biotoxins. We can report nine new vectors for these toxins in the North Atlantic: Astropecten aranciacus, Arbacia lixula, Echinaster sepositus, Holothuria sanctori, Ophidiaster ophidianus, Onchidella celtica, Aplysia depilans, Patella spp., and Stramonita haemostoma. Differences in toxin contents among the species were found. Even though low concentrations were detected, the levels of toxins that were present, especially in edible species, indicate the importance of these types of studies. Routine monitoring should be extended to comprise a wider number of vectors other than for bivalves of okadaic acid and its analogues. PMID:26670254

  17. Phylogenetic Tree Analysis of the Cold-Hot Nature of Traditional Chinese Marine Medicine for Possible Anticancer Activity

    PubMed Central

    Song, Xuxia; Li, Xuebo; Zhang, Fengcong; Wang, Changyun

    2017-01-01

    Traditional Chinese Marine Medicine (TCMM) represents one of the medicinal resources for research and development of novel anticancer drugs. In this study, to investigate the presence of anticancer activity (AA) displayed by cold or hot nature of TCMM, we analyzed the association relationship and the distribution regularity of TCMMs with different nature (613 TCMMs originated from 1,091 species of marine organisms) via association rules mining and phylogenetic tree analysis. The screened association rules were collected from three taxonomy groups: (1) Bacteria superkingdom, Phaeophyceae class, Fucales order, Sargassaceae family, and Sargassum genus; (2) Viridiplantae kingdom, Streptophyta phylum, Malpighiales class, and Rhizophoraceae family; (3) Holothuroidea class, Aspidochirotida order, and Holothuria genus. Our analyses showed that TCMMs with closer taxonomic relationship were more likely to possess anticancer bioactivity. We found that the cluster pattern of marine organisms with reported AA tended to cluster with cold nature TCMMs. Moreover, TCMMs with salty-cold nature demonstrated properties for softening hard mass and removing stasis to treat cancers, and species within Metazoa or Viridiplantae kingdom of cold nature were more likely to contain AA properties. We propose that TCMMs from these marine groups may enable focused bioprospecting for discovery of novel anticancer drugs derived from marine bioresources. PMID:28191021

  18. 78 FR 2439 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Appeals of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-11

    ...] Agency Information Collection Activities: Proposed Collection; Comments Requested; Appeals of Background...., permitting electronic submission of responses. Summary of Information Collection (1) Type of Information Collection: Extension of a currently approved collection. (2) Title of the Form/Collection: Appeals of...

  19. Collection analysis techniques used to evaluate a graduate-level toxicology collection.

    PubMed

    Crawley-Low, Jill V

    2002-07-01

    Collections librarians from academic libraries are often asked, on short notice, to evaluate whether their collections are able to support changes in their institutions' curricula, such as new programs or courses or revisions to existing programs or courses. With insufficient time to perform an exhaustive critique of the collection and a need to prepare a report for faculty external to the library, a selection of reliable but brief qualitative and quantitative tests is needed. In this study, materials-centered and use-centered methods were chosen to evaluate the toxicology collection of the University of Saskatchewan (U of S) Library. Strengths and weaknesses of the techniques are reviewed, along with examples of their use in evaluating the toxicology collection. The monograph portion of the collection was evaluated using list checking, citation analysis, and classified profile methods. Cost-effectiveness and impact factor data were compiled to rank journals from the collection. Use-centered methods such as circulation and interlibrary loan data identified highly used items that should be added to the collection. Finally, although the data were insufficient to evaluate the toxicology electronic journals at the U of S, a brief discussion of three initiatives that aim to assist librarians as they evaluate the use of networked electronic resources in their collections is presented.

  20. Growing Mathematical Understanding through Collective Image Making, Collective Image Having, and Collective Property Noticing

    ERIC Educational Resources Information Center

    Martin, Lyndon C.; Towers, Jo

    2015-01-01

    In the research reported in this paper, we develop a theoretical perspective to describe and account for the growth of collective mathematical understanding. We discuss collective processes in mathematics, drawing in particular on theoretical work in the domains of improvisational jazz and theatre. Using examples of data from a study of elementary…

  1. 78 FR 66382 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Request by...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-05

    ... Information Collection Activities; Proposed Collection; Comments Requested: Request by Organization for... Information Collection: New Voluntary Collection. (2) Title of the Form/Collection: Request by Organization... information collection request to the Office of Management and Budget (OMB) for review and approval in...

  2. Collection-Level Surveys for Special Collections: Coalescing Descriptors across Standards

    ERIC Educational Resources Information Center

    Ascher, James P.; Ferris, Anna M.

    2012-01-01

    Developing collection-level surveys to expose hidden collections in special collections and archives departments within ARL libraries has received a great deal of scholarly attention in the recent years. Numerous standards have been explored, and each has its strengths and weaknesses. This paper summarizes some of the major initiatives in…

  3. Collection time again.

    PubMed

    Elder, D L

    1984-01-01

    Looking forward to your next round of collection calls? Few of us do, yet collections play a vital role in every group practice, large or small. This crop of practical collection tips is guaranteed to boost the morale of everyone involved in the collection process. Not only are the basics of a good collection call set down, but result-getting responses to frequent debtor excuses are provided. Telephoning the debtor patient need not be a dreaded task. Just have confidence, keep in mind that the patient does owe you for services rendered in good faith, and pick up the phone--it's a snap!

  4. 77 FR 66634 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Appeals of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-06

    ...] Agency Information Collection Activities: Proposed Collection; Comments Requested: Appeals of Background... Information Collection (1) Type of Information Collection: Extension of a currently approved collection. (2) Title of the Form/Collection: Appeals of Background Checks. (3) Agency form number, if any, and the...

  5. 76 FR 40935 - Agency Information Collection Activities: Proposed Collection Comments Requested Race and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-12

    ...] Agency Information Collection Activities: Proposed Collection Comments Requested Race and National Origin... collection. (2) Title of the Form/Collection: Race and National Origin Identification. (3) Agency form number.... Other: none. Need for Collection: The information collection is used to maintain Race and National...

  6. 75 FR 65382 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-22

    ... of Information Collection Under Review: Implementation of Public Law 103-322, The Violent Crime... collection. (2) Title of the Form/Collection: Implementation of Public Law 103- 322, The Violent Crime...

  7. 76 FR 16790 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-25

    ... of Information Collection Request: New collection; Title of Information Collection: Autism Spectrum Disorders (ASD): State of the States Services and Supports for People with ASD; Use: The information that is...

  8. Zoological Collections and Collecting in Cuba during the Twentieth Century.

    ERIC Educational Resources Information Center

    Taboada, Gilberto Silva

    1994-01-01

    Traces the history of 20th-century zoological collections in Cuba, and the present whereabouts of Cuba's zoological collections. The historical accounts are divided into two periods: from 1902 to 1959 and from 1959 to the present. A preliminary survey of the nature, size, and current state of these collections is included. (MDH)

  9. 7 CFR 1250.516 - Collecting handlers and collection.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... RESEARCH AND PROMOTION Rules and Regulations Assessments, Collections, and Remittances § 1250.516... who grades, cartons, or breaks such eggs. Such shell egg breaker or egg packer must collect and remit... person who buys or receives nest run eggs from a producer and who does not grade, carton, or break such...

  10. 7 CFR 1250.516 - Collecting handlers and collection.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... RESEARCH AND PROMOTION Rules and Regulations Assessments, Collections, and Remittances § 1250.516... who grades, cartons, or breaks such eggs. Such shell egg breaker or egg packer must collect and remit... person who buys or receives nest run eggs from a producer and who does not grade, carton, or break such...

  11. 7 CFR 1250.516 - Collecting handlers and collection.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... RESEARCH AND PROMOTION Rules and Regulations Assessments, Collections, and Remittances § 1250.516... who grades, cartons, or breaks such eggs. Such shell egg breaker or egg packer must collect and remit... person who buys or receives nest run eggs from a producer and who does not grade, carton, or break such...

  12. 7 CFR 1250.516 - Collecting handlers and collection.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... RESEARCH AND PROMOTION Rules and Regulations Assessments, Collections, and Remittances § 1250.516... who grades, cartons, or breaks such eggs. Such shell egg breaker or egg packer must collect and remit... person who buys or receives nest run eggs from a producer and who does not grade, carton, or break such...

  13. 7 CFR 1250.516 - Collecting handlers and collection.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... RESEARCH AND PROMOTION Rules and Regulations Assessments, Collections, and Remittances § 1250.516... who grades, cartons, or breaks such eggs. Such shell egg breaker or egg packer must collect and remit... person who buys or receives nest run eggs from a producer and who does not grade, carton, or break such...

  14. 76 FR 45861 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Generic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-01

    ... DEPARTMENT OF JUSTICE [OMB Number 1103-NEW] Agency Information Collection Activities: Proposed Collection; Comments Requested; Generic Information Collection Review of Customer Outreach and Information ACTION: 30-Day Notice of Information Collection Under Review. [[Page 45862

  15. 78 FR 76860 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Inventories...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ...] Agency Information Collection Activities; Proposed Collection; Comments Requested: Inventories, Licensed... currently approved collection. (2) Title of the Form/Collection: Inventories, Licensed Explosives Importers... explosive material inventories of those persons engaged in various activities within the explosives industry...

  16. 75 FR 30858 - Justice Management Division; Agency Information Collection Activities: Proposed Collection...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-02

    ... DEPARTMENT OF JUSTICE [OMB Number 1103-0018] Justice Management Division; Agency Information Collection Activities: Proposed Collection: Common Request ACTION: 60-Day Notice of Information Collection Under Review: Extension of Previously Approved Collection; Department of Justice Procurement Blanket...

  17. 77 FR 59665 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Voluntary...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-28

    ... DEPARTMENT OF JUSTICE Bureau of Alcohol, Tobacco, Firearms and Explosives [OMB Notice 1140-0092] Agency Information Collection Activities; Proposed Collection; Comments Requested: Voluntary Magazine... collection. (2) Title of the Form/Collection: Voluntary Magazine Questionnaire for Agencies/Entities Who...

  18. Disposable collection kit for rapid and reliable collection of saliva.

    PubMed

    Yamaguchi, Masaki; Tezuka, Yuki; Takeda, Kazunori; Shetty, Vivek

    2015-01-01

    To describe and evaluate disposable saliva collection kit for rapid, reliable, and reproducible collection of saliva samples. The saliva collection kit comprised of a saliva absorbent swab and an extractor unit was used to retrieve whole saliva samples from 10 subjects. The accuracy and precision of the extracted volumes (3, 10, and 30 μl) were compared to similar volumes drawn from control samples obtained by passive drool. Additionally, the impact of kit collection method on subsequent immunoassay results was verified by assessing salivary cortisol levels in the samples and comparing them to controls. The recovered volumes for the whole saliva samples were 3.85 ± 0.28, 10.79 ± 0.95, and 31.18 ± 1.72 μl, respectively (CV = 8.76%) and 2.91 ± 0.19, 9.75 ± 0.43, and 29.64 ± 0.91 μl, respectively, (CV = 6.36%) for the controls. There was a close correspondence between the salivary cortisol levels from the saliva samples obtained by the collection kit and the controls (R(2)  > 0.96). The disposable saliva collection kit allows accurate and repeatable collection of fixed amounts of whole saliva and does not interfere with subsequent measurements of salivary cortisol. The simple collection process, lack of elaborate specimen recovery steps, and the short turnaround time (<3 min) should render the kit attractive to test subjects and researchers alike. © 2015 Wiley Periodicals, Inc.

  19. Disposable Collection Kit for Rapid and Reliable Collection of Saliva

    PubMed Central

    Yamaguchi, Masaki; Tezuka, Yuki; Takeda, Kazunori; Shetty, Vivek

    2015-01-01

    Objectives To describe and evaluate disposable saliva collection kit for rapid, reliable, and reproducible collection of saliva samples. Methods The saliva collection kit comprised of a saliva absorbent swab and an extractor unit was used to retrieve whole saliva samples from 10 subjects. The accuracy and precision of the extracted volumes (3, 10, and 30 μl) were compared to similar volumes drawn from control samples obtained by passive drool. Additionally, the impact of kit collection method on subsequent immunoassay results was verified by assessing salivary cortisol levels in the samples and comparing them to controls. Results The recovered volumes for the whole saliva samples were 3.85 ± 0.28, 10.79 ± 0.95, and 31.18 ± 1.72 μl, respectively (CV = 8.76%) and 2.91 ± 0.19, 9.75 ± 0.43, and 29.64 ± 0.91 μl, respectively, (CV = 6.36%) for the controls. There was a close correspondence between the salivary cortisol levels from the saliva samples obtained by the collection kit and the controls (R2 > 0.96). Conclusions The disposable saliva collection kit allows accurate and repeatable collection of fixed amounts of whole saliva and does not interfere with subsequent measurements of salivary cortisol. The simple collection process, lack of elaborate specimen recovery steps, and the short turnaround time (<3 min) should render the kit attractive to test subjects and researchers alike. Am. J. Hum. Biol. 27:720–723, 2015. © 2015 The Authors American Journal of Human Biology Published by Wiley Periodicals, Inc. PMID:25754371

  20. 78 FR 31563 - Agency Information Collection Activities; Proposed Collection; Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-24

    ... collecting, validating and verifying information, processing and maintaining information, and disclosing and... Information Collection Activities; Proposed Collection; Public Comment Request AGENCY: Health Resources and... utility of the proposed information collection for the proper performance of the agency's functions, (2...

  1. 76 FR 16393 - Commission Information Collection Activities, Proposed Collection; Comment Request; Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-23

    ...] Commission Information Collection Activities, Proposed Collection; Comment Request; Extension AGENCY: Federal Energy Regulatory Commission, DOE. ACTION: Notice of proposed information collection and request for... public comment on the specific aspects of the information collection described below. DATES: Comments in...

  2. 78 FR 68846 - Agency Information Collection Activities; Proposed Collection; Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-15

    ... systems for the purpose of collecting, validating and verifying information, processing and maintaining...-30D] Agency Information Collection Activities; Proposed Collection; Public Comment Request AGENCY..., announces plans to submit an Information Collection Request (ICR), described below, to the Office of...

  3. 75 FR 33319 - Agency Information Collection Activities: New Information Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-11

    ... Information Collection; ICE Mutual Agreement Between Government and Employers (IMAGE). The Department of... technological collection techniques or other forms of information technology, e.g., permitting electronic... information collection. (2) Title of the Form/Collection: ICE Mutual Agreement between Government and...

  4. 76 FR 30389 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Generic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-25

    ... DEPARTMENT OF JUSTICE [OMB Number 1103-NEW] Agency Information Collection Activities: Proposed Collection; Comments Requested; Generic Information Collection Review of Customer Outreach and Information ACTION: 60-Day Notice of Information Collection Under Review. The Department of Justice (DOJ) Office of...

  5. 77 FR 36294 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Accounting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-18

    ... Collection Activities; Proposed Collection; Comments Requested: Accounting System and Financial Capability... change of a currently approved collection. (2) The title of the form/collection: Accounting System and... for-profit entities and not-for-profit institutions. Other: None. The purpose of the Accounting System...

  6. 75 FR 39972 - Agency Information Collection Activities: Proposed collection; comments requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-13

    ... DEPARTMENT OF JUSTICE Justice Management Division [OMB Number 1103-0016] Agency Information Collection Activities: Proposed collection; comments requested ACTION: 60-Day Notice of Information Collection Under Review: Certification of Identity. The Department of Justice (DOJ), Justice Management...

  7. Urine collection device

    NASA Technical Reports Server (NTRS)

    Michaud, R. B. (Inventor)

    1981-01-01

    A urine collection device for females is described. It is comprised of a collection element defining a urine collection chamber and an inlet opening into the chamber and is adapted to be disposed in surrounding relation to the urethral opening of the user. A drainage conduit is connected to the collection element in communication with the chamber whereby the chamber and conduit together comprise a urine flow pathway for carrying urine generally away from the inlet. A first body of wicking material is mounted adjacent the collection element and extends at least partially into the flow pathway. The device preferably also comprise a vaginal insert element including a seal portion for preventing the entry of urine into the vagina.

  8. 76 FR 6633 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-07

    ... of Information Collection Under Review: Customer Satisfaction Surveys. The Department of Justice (DOJ...: Extension of a currently approved collection. (2) Title of the Form/Collection: Customer Satisfaction... of Alcohol, Tobacco, Firearms and Explosives distribute program-specific customer satisfaction...

  9. 76 FR 66086 - Agency Information Collection Activities; Proposed Collection, Comments Requested: Reinstatement...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-25

    ... Approved Collection for Which Approval Has Expired, Identity Theft Supplement (ITS) to the National Crime... approved collection for which approval has expired. (2) Title of the Form/Collection: Identity Theft... United States. The Identity Theft Supplement (ITS) to the National Crime Victimization Survey collects...

  10. 75 FR 39533 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-09

    ... information dissemination activities. The purpose of this submission is to extend the existing generic... Agency Information Collection Activities: Proposed Collection; Comment Request In compliance with Section... proposed collections of information, the Substance Abuse and Mental Health Services Administration (SAMHSA...

  11. 76 FR 36155 - Agency Information Collection Activities: Proposed Collection, Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-21

    ... Collection Under Review: Establishment and distribution of National Fingerprint Examiners Questionnaire. The...) Type of information collection: Identify agencies that provide fingerprint comparisons. (2) The title of the form/collection: National Fingerprint Examiners Questionnaire. (3) The agency form number, if...

  12. 76 FR 40773 - Agency Information Collection Activity; Proposed Collection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-11

    ... DEPARTMENT OF THE TREASURY Internal Revenue Service Agency Information Collection Activity; Proposed Collection AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for...: Direct all written comments to Yvette Lawrence, Internal Revenue Service, room 6129, 1111 Constitution...

  13. 76 FR 40775 - Agency Information Collection Activity; Proposed Collection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-11

    ... DEPARTMENT OF THE TREASURY Internal Revenue Service Agency Information Collection Activity; Proposed Collection AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for... written comments to Yvette Lawrence, Internal Revenue Service, Room 6129, 1111 Constitution Avenue, NW...

  14. 77 FR 4404 - Agency Information Collection Activity; Proposed Collection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-27

    ... DEPARTMENT OF THE TREASURY Internal Revenue Service Agency Information Collection Activity; Proposed Collection AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for... Lawrence, Internal Revenue Service, room 6129, 1111 Constitution Avenue NW., Washington, DC 20224. FOR...

  15. EpiCollect+: linking smartphones to web applications for complex data collection projects

    PubMed Central

    Aanensen, David M.; Huntley, Derek M.; Menegazzo, Mirko; Powell, Chris I.; Spratt, Brian G.

    2014-01-01

    Previously, we have described the development of the generic mobile phone data gathering tool, EpiCollect, and an associated web application, providing two-way communication between multiple data gatherers and a project database. This software only allows data collection on the phone using a single questionnaire form that is tailored to the needs of the user (including a single GPS point and photo per entry), whereas many applications require a more complex structure, allowing users to link a series of forms in a linear or branching hierarchy, along with the addition of any number of media types accessible from smartphones and/or tablet devices (e.g., GPS, photos, videos, sound clips and barcode scanning). A much enhanced version of EpiCollect has been developed (EpiCollect+). The individual data collection forms in EpiCollect+ provide more design complexity than the single form used in EpiCollect, and the software allows the generation of complex data collection projects through the ability to link many forms together in a linear (or branching) hierarchy. Furthermore, EpiCollect+ allows the collection of multiple media types as well as standard text fields, increased data validation and form logic. The entire process of setting up a complex mobile phone data collection project to the specification of a user (project and form definitions) can be undertaken at the EpiCollect+ website using a simple ‘drag and drop’ procedure, with visualisation of the data gathered using Google Maps and charts at the project website. EpiCollect+ is suitable for situations where multiple users transmit complex data by mobile phone (or other Android devices) to a single project web database and is already being used for a range of field projects, particularly public health projects in sub-Saharan Africa. However, many uses can be envisaged from education, ecology and epidemiology to citizen science. PMID:25485096

  16. EpiCollect+: linking smartphones to web applications for complex data collection projects.

    PubMed

    Aanensen, David M; Huntley, Derek M; Menegazzo, Mirko; Powell, Chris I; Spratt, Brian G

    2014-01-01

    Previously, we have described the development of the generic mobile phone data gathering tool, EpiCollect, and an associated web application, providing two-way communication between multiple data gatherers and a project database. This software only allows data collection on the phone using a single questionnaire form that is tailored to the needs of the user (including a single GPS point and photo per entry), whereas many applications require a more complex structure, allowing users to link a series of forms in a linear or branching hierarchy, along with the addition of any number of media types accessible from smartphones and/or tablet devices (e.g., GPS, photos, videos, sound clips and barcode scanning). A much enhanced version of EpiCollect has been developed (EpiCollect+). The individual data collection forms in EpiCollect+ provide more design complexity than the single form used in EpiCollect, and the software allows the generation of complex data collection projects through the ability to link many forms together in a linear (or branching) hierarchy. Furthermore, EpiCollect+ allows the collection of multiple media types as well as standard text fields, increased data validation and form logic. The entire process of setting up a complex mobile phone data collection project to the specification of a user (project and form definitions) can be undertaken at the EpiCollect+ website using a simple 'drag and drop' procedure, with visualisation of the data gathered using Google Maps and charts at the project website. EpiCollect+ is suitable for situations where multiple users transmit complex data by mobile phone (or other Android devices) to a single project web database and is already being used for a range of field projects, particularly public health projects in sub-Saharan Africa. However, many uses can be envisaged from education, ecology and epidemiology to citizen science.

  17. 77 FR 32136 - Agency Information Collection Activities: Proposed collection; Comments Requested; Federal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-31

    ... Licensee Firearms Inventory Theft/ Loss Report ACTION: 30-Day Notice of Information Collection Under Review... approved collection. (2) Title of the Form/Collection: Federal Firearms Licensee Firearms Inventory Theft... authorities any theft or loss of a firearm from the licensee's inventory or collection within a specific time...

  18. 77 FR 17500 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ... DEPARTMENT OF JUSTICE [OMB Number 1105-0025] Agency Information Collection Activities: Proposed Collection; Comments Requested ACTION: 30-Day Notice of Information Collection Under Review: Federal Coal Lease Request. The Department of Justice (DOJ), Antitrust Division (ATR), will be submitting the...

  19. 75 FR 74082 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-30

    ... of information collection under review: customer satisfaction surveys. The Department of Justice (DOJ... collection. (2) Title of the Form/Collection: Customer Satisfaction Surveys. (3) Agency form number, if any... program-specific customer satisfaction surveys to more effectively capture customer perception...

  20. 76 FR 18222 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-01

    ... proposed information collection for the proper performance of the agency's functions; (2) the accuracy of... currently approved collection; Title of Information Collection: Independent Renal Dialysis Facility Cost Report; Use: The Independent Renal Dialysis Facility Cost Report, is filed annually by providers...

  1. 76 FR 62413 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-07

    ... proposed information collection for the proper performance of the agency's functions; (2) the accuracy of... currently approved collection; Title of Information Collection: Consolidated Renal Operations in a Web Enabled Network (CROWNWeb) Third-party Submission Authorization Form; Use: The Consolidated Renal...

  2. 76 FR 31988 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-02

    ... DEPARTMENT OF JUSTICE Office of Community Oriented Policing Services [OMB Number 1103-NEW] Agency Information Collection Activities: Proposed Collection; Comments Requested ACTION: 30-Day Notice of Information Collection under Review: COPS Police and Communities Together (PACT) 360 Needs Assessment Survey...

  3. Collection Development Project.

    ERIC Educational Resources Information Center

    Nuby, Mary

    Undertaken to provide data on the current status of collection development in selected academic libraries, this study also analyzed the structure of the collection development function at Chicago Academic Library Council (CALC) institutions and outlined a formal collection development policy for Chicago State University's Douglas Library.…

  4. 78 FR 48135 - Agency Information Collection Activities: Proposed Collection;

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-07

    .... ADDRESSES: Comments should be sent to Richard Lucas, Office of Policy Support, Food and Nutrition Service, U... DEPARTMENT OF AGRICULTURE Food and Nutrition Service Agency Information Collection Activities: Proposed Collection; Comment Request--Enhancing Completion Rates for Supplemental Nutrition Assistance...

  5. Diversity and antimicrobial potential in sea anemone and holothurian microbiomes.

    PubMed

    León-Palmero, Elizabeth; Joglar, Vanessa; Álvarez, Pedro A; Martín-Platero, Antonio; Llamas, Inmaculada; Reche, Isabel

    2018-01-01

    Marine invertebrates, as holobionts, contain symbiotic bacteria that coevolve and develop antimicrobial substances. These symbiotic bacteria are an underexplored source of new bioactive molecules to face the emerging antibiotic resistance in pathogens. Here, we explored the antimicrobial activity of bacteria retrieved from the microbiota of two sea anemones (Anemonia sulcata, Actinia equina) and two holothurians (Holothuria tubulosa, Holothuria forskali). We tested the antimicrobial activity of the isolated bacteria against pathogens with interest for human health, agriculture and aquaculture. We isolated 27 strains with antibacterial activity and 12 of these isolates also showed antifungal activity. We taxonomically identified these strains being Bacillus and Vibrio species the most representative producers of antimicrobial substances. Microbiome species composition of the two sea anemones was similar between them but differed substantially of seawater bacteria. In contrast, microbiome species composition of the two holothurian species was different between them and in comparison with the bacteria in holothurian feces and seawater. In all the holobiont microbiomes Bacteroidetes was the predominant phylum. For each microbiome, we determined diversity and the rank-abundance dominance using five fitted models (null, pre-emption, log-Normal, Zipf and Zipf-Mandelbrot). The models with less evenness (i.e. Zipf and Zipf-Mandelblot) showed the best fits in all the microbiomes. Finally, we tracked (using the V4 hypervariable region of 16S rRNA gene) the relative abundance of these 27 isolates with antibacterial activity in the total pool of sequences obtained for the microbiome of each holobiont. Coincidences, although with extremely low frequencies, were detected only in the microbiome of H. forskali. This fact suggests that these isolated bacteria belong to the long tail of rare symbiotic bacteria. Therefore, more and more sophisticated culture techniques are necessary

  6. Inorganic carbon turnover caused by digestion of carbonate sands and metabolic activity of holothurians

    NASA Astrophysics Data System (ADS)

    Schneider, Kenneth; Silverman, Jacob; Kravitz, Ben; Rivlin, Tanya; Schneider-Mor, Aya; Barbosa, Sergio; Byrne, Maria; Caldeira, Ken

    2013-11-01

    Recent measurements have shown that holothurians (sea cucumbers) may play an important role in the cycling of CaCO3 in tropical coral reef systems through ingestion and processing of carbonate sediment. In this report, we present estimates of inorganic carbon turnover rates determined from laboratory incubations of Holothuria atra, Holothuria leucospilota and Stichopus herrmanni. The pH values of the gut lumen ranged from 7.0 to 7.6 when digestive tracts were filled with sediment compared with 6.1-6.7 in animals with empty digestive tracts. Empty gut volume estimates for H. atra and S. herrmanni were 36 ± 4 mL and 151 ± 14 mL, respectively. Based on these measurements and the density and porosity of carbonate sediments of coral reefs, it is estimated that these species process 19 ± 2 kg and 80 ± 7 kg CaCO3 sand yr-1 per individual, respectively. The annual CaCO3 dissolution rates per H. atra and S. herrmanni individual are estimated to be 6.5 ± 1.9 g and 9.6 ± 1.4 g, respectively, suggesting that 0.05 ± 0.02% and 0.1 ± 0.02% of the CaCO3 processed through their gut annually is dissolved. During incubations the CaCO3 dissolution of the fecal casts was 0.07 ± 0.01%, 0.04 ± 0.01% and 0.21 ± 0.05% for H. atra, H. leucospilota and S. herrmanni, respectively. The CaCO3 saturation state in the incubation seawater decreased markedly due to a greater increase in dissolved inorganic carbon (DIC) relative to total alkalinity (AT) as a result of respiration by the animals. Our results support the hypothesis that deposit feeders such as sea cucumbers play an important ecological role in the coral reef CaCO3 cycle.

  7. 77 FR 5845 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Report of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-06

    ...] Agency Information Collection Activities: Proposed Collection; Comments Requested: Report of Theft or... currently approved collection. (2) Title of the Form/Collection: Report of Theft or Loss of Explosives. (3... for-profit. Other: None. Need for Collection Losses or theft of explosives must, by statute be...

  8. 77 FR 54612 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-05

    ... Information Collection Activities: Proposed Collection; Comments Requested: Notice of Appeal to the Board of Immigration Appeals From a Decision of a DHS Officer ACTION: 30-Day notice of information collection. The... This Information Collection (1) Type of Information Collection: Extension of a Currently Approved...

  9. 75 FR 3758 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-22

    ... Collection Under Review: International Terrorism Victim Expense Reimbursement Program Application. The... which approval has expired. (2) The title of the form/collection: International Terrorism Victim Expense... international terrorism that occur(red) outside of the United States. The application will be used to collect...

  10. 76 FR 39906 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-07

    ... collection is required: There is a one-time application for any licensee wishing to renew its nuclear power... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2011-0130] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission (NRC). ACTION: Notice of pending...

  11. 75 FR 37841 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-30

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2010-0214] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: U.S. Nuclear Regulatory Commission (NRC). ACTION: Notice of... the collection is required: Applications for new licenses and amendments may be submitted at any time...

  12. 75 FR 67119 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-01

    ... of Information Collection Under Review: Prevent All Cigarette Trafficking (PACT) Act Registration...: Extension of a currently approved collection. (2) The title of the form/collection: Prevent All Cigarette... register delivery sellers of cigarettes and/or smokeless tobacco products with the Attorney General in...

  13. 77 FR 1778 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-11

    ... TENNESSEE VALLEY AUTHORITY Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Tennessee Valley Authority. ACTION: 30-Day notice of submission of information collection... Act of 1995 (44 U.S.C. chapter 35, as amended). The Tennessee Valley Authority is soliciting public...

  14. 76 FR 38718 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-01

    ... TENNESSEE VALLEY AUTHORITY Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Tennessee Valley Authority. ACTION: 60-Day notice of submission of information collection... Act of 1995 (44 U.S.C. Chapter 35, as amended). The Tennessee Valley Authority is soliciting public...

  15. 76 FR 44962 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-27

    ... Information: Analysis of the Facilities Survey data will provide updated information on the status of... NATIONAL SCIENCE FOUNDATION Agency Information Collection Activities: Proposed Collection; Comment... information collection. The full submission may be found at: http://www.reginfo.gov/public/do/PRAMain . This...

  16. 78 FR 53465 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-29

    ... collection of qualitative data regarding preparedness message framing. The goal of this qualitative research... Message Framing Research. Type of Information Collection: New information collection. FEMA Forms: 008-0-17... will engage in qualitative research involving the review of disaster preparedness message frames for...

  17. 77 FR 12847 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-02

    ... Information Technology Systems, and awardees under the Cooperative Agreement to Support Establishment of State... Identifier: CMS-10424] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY... to send comments regarding this burden estimate or any other aspect of this collection of information...

  18. 77 FR 61829 - Proposed Collection; Comment Request for Information Collection Tools

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-11

    ... information on respondents, including through the use of automated collection techniques or other forms of... Information Collection Tools AGENCY: Internal Revenue Service (IRS), Treasury. ACTION: Notice and request for... on proposed and/or continuing information collections, as required by the Paperwork Reduction Act of...

  19. 77 FR 38297 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-27

    ... Identifier CMS-359 and -360] Agency Information Collection Activities: Proposed Collection; Comment Request... information, including any of the following subjects: (1) The necessity and utility of the proposed information collection for the proper performance of the agency's functions; (2) the accuracy of the estimated...

  20. 76 FR 18581 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-04

    ... collected will include the types of criminal cases appealed to state intermediate appellate courts and... collection: Survey of State Court Criminal Appeals, 2010. The Department of Justice, Office of Justice... electronic submission of responses. Overview of This Information (1) Type of information collection: New data...

  1. 75 FR 65510 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-25

    ... of Information Collection Under Review: Permanent Provisions of the Brady Handgun Violence Prevention... collection. (2) Title of the Form/Collection: Permanent Provisions of the Brady Handgun Violence Prevention... Violence Prevention Act. (6) An estimate of the total public burden (in hours) associated with the...

  2. 77 FR 59615 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-28

    ...) ways to enhance the quality, utility, and clarity of the information to be collected; and (4) the use... Public: Private Sector: Business or other for-profit and not- for-profit institutions; Public Sector... Identifier CMS-10408] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY...

  3. 75 FR 1812 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-13

    ... Under Review: Extension of a currently approved collection; Bulletproof Vest Partnership. The Department...: Extension of a currently approved collection. (2) The title of the form/collection: Bulletproof Vest... Vest Partnership (BVP) Program. The purpose of this program is to help protect the lives of law...

  4. 75 FR 80846 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-23

    ... of Information Collection Under Review: Application For Tax Exempt Transfer and Registration of...: Extension of a currently approved collection. (2) Title of the Form/Collection: Application For Tax Exempt... Firearms Act (NFA) firearm exempt from transfer tax based on statutory exemptions. The information on the...

  5. 77 FR 39261 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-02

    ... Collection; Comments Requested: Notice of Appeal to the Board of Immigration Appeals From a Decision of a DHS.... Overview of This Information Collection (1) Type of Information Collection: Revision of a currently approved collection. (2) Title of the Form/Collection: Notice of Appeal to the Board of Immigration Appeals...

  6. 76 FR 2690 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-14

    ... information on the CMS' public Internet Web site concerning a self-referral disclosure protocol (SRDP) that... participate in the SRDP. The SRDP is a voluntary self-disclosure instrument that will allow providers of... Collection Request: Revision of currently approved collection; Title of Information Collection: Medicare Self...

  7. 78 FR 78373 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-26

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Substance Abuse and Mental Health Services Administration Agency Information Collection Activities: Proposed Collection; Comment Request In compliance with Section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995 concerning opportunity for public comment on proposed collections of information, the...

  8. 77 FR 20437 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-04

    ... there a way to enhance the quality, utility, and clarity of the information to be collected? 4. How can... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2012-0027] Agency Information Collection Activities... action to submit an information collection request to the Office of Management and Budget (OMB) and...

  9. 78 FR 48687 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-09

    ... of Drug Pricing and Network Pharmacy Data from Medicare Prescription Drug Plans (PDPs and MA-PDs) and... children, by age group and basis of Medicaid eligibility, who are provided initial and periodic child... previously approved collection; Title of Information Collection: Collection of Drug Pricing and Network...

  10. 78 FR 38986 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-28

    ... that information was collected under Part B. The QIMS Account Registration and the ESRD Application..., CMS-1728-94, CMS-10174, CMS-10305 and CMS-10488] Agency Information Collection Activities: Proposed... comment on CMS' intention to collect information from the public. Under the Paperwork Reduction Act of...

  11. 78 FR 39296 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-01

    ... & Medicaid Services (CMS) is announcing an opportunity for the public to comment on CMS' intention to collect information from the public. Under the Paperwork Reduction Act of 1995 (PRA), federal agencies are required to... Identifier: CMS-10486] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY...

  12. 77 FR 34959 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-12

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Substance Abuse and Mental Health Services Administration Agency Information Collection Activities: Proposed Collection; Comment Request In compliance with Section 3506(c)(2)(A) of the Paperwork Reduction Act of 1995 concerning opportunity for public comment on proposed collections of information, the...

  13. 77 FR 61007 - Agency Information Collection Activities; Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-05

    ... information; (c) ways to enhance the quality, utility, and clarity of the information to be collected; and (d... funding that would potentially impact the environment and to ensure that their decision- making processes... Information Collection Activities; Proposed Collection: Comment Request In compliance with the requirement for...

  14. 78 FR 67149 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-08

    ... Identifier: CMS-R-216] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY... & Medicaid Services (CMS) is announcing an opportunity for the public to comment on CMS' intention to collect... accepting comments. 2. By regular mail. You may mail written comments to the following address: CMS, Office...

  15. 75 FR 64355 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-19

    ... of Information Collection Under Review: Application for Tax-Exempt Transfer of Firearm and... collection. (2) Title of the Form/Collection: Application for Tax-Exempt Transfer of Firearm and Registration... approved by ATF prior to the transfer of a National Firearms Act weapon from one Special Occupational Tax...

  16. 75 FR 41868 - Agency Information Collection Activities: Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Health Resources and Services Administration Agency Information Collection Activities: Proposed Collection: Comment Request In compliance with the requirement for opportunity for public comment on proposed data collection projects (section 3506(c)(2)(A) of title 44, United States Code, as amended by the...

  17. 76 FR 64944 - Agency Information Collection Activities: Final Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-19

    ... EXPORT-IMPORT BANK OF THE U.S. [Public Notice 2011-076] Agency Information Collection Activities: Final Collection; Comment Request AGENCY: Export-Import Bank of the U.S. ACTION: Submission for OMB... between a U.S. exporter, Ex-Im Bank, and a foreign export credit agency; the information collected...

  18. 77 FR 284 - Agency Information Collection Activities: Final Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-04

    ... EXPORT-IMPORT BANK OF THE U.S. [Public Notice 2011-0080] Agency Information Collection Activities: Final Collection; Comment Request AGENCY: Export-Import Bank of the U.S. ACTION: Submission for OMB... between a U.S. exporter, Ex-Im Bank, and a foreign export credit agency; the information collected...

  19. 76 FR 73648 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-29

    ... burden; (3) ways to enhance the quality, utility, and clarity of the information to be collected; and (4... collection; Title of Information Collection: Request for Certification as a Rural Health Clinic Form and... of Rural Health Clinic (RHC) Services under the Medicare/ Medicaid Program, is utilized as an...

  20. 75 FR 18885 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-13

    ... of information collection under review: Police check inquiry. The Department of Justice (DOJ), Bureau... approved collection. (2) Title of the Form/Collection: Police Check Inquiry. (3) Agency form number, if any... permit ATF to complete and/or initiate a police check inquiry consisting of criminal record searches. In...

  1. 75 FR 76473 - Agency Information Collection Activities: Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-08

    ... technology. Proposed Project: Data Collection Tool for Rural Health Community-Based Grant Programs (OMB No... based on the feedback received from grantees and to reflect ORHP and HRSA's current priorities and... collection and analysis in an effort to strengthen the value of the data collection tool. There are currently...

  2. Collecting Rocks.

    ERIC Educational Resources Information Center

    Barker, Rachel M.

    One of a series of general interest publications on science topics, the booklet provides those interested in rock collecting with a nontechnical introduction to the subject. Following a section examining the nature and formation of igneous, sedimentary, and metamorphic rocks, the booklet gives suggestions for starting a rock collection and using…

  3. 76 FR 15997 - Information Collection Activities; Proposed Information Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ... following approved information collection set to expire on August 31, 2011: Recreation Visitor Use Surveys... submitting the information collection request--OMB No. 1006- 0028--to the Office of Management and Budget... collection of visitor use data, we will be requesting that OMB reauthorize a two-part request. The first part...

  4. 76 FR 11444 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-02

    ... Natural Gas Data Collection Program Package. The surveys covered by this request include: Form EIA-176... burden for the surveys included in the Natural Gas Data Collection Program Package is shown below as an... help the EIA prepare data requests that maximize the utility of the information collected and assess...

  5. 77 FR 32137 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Strategic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-31

    ...] Agency Information Collection Activities: Proposed Collection; Comments Requested; Strategic Planning... Form/Collection: Strategic Planning Environmental Assessment Outreach. (3) Agency form number, if any... Strategic Planning Office at ATF will [[Page 32138

  6. Collection Analysis: Powerful Ways To Collect, Analyze, and Present Your Data.

    ERIC Educational Resources Information Center

    Hart, Amy

    2003-01-01

    Discussion of collection analysis in school libraries focuses on the kinds of data used and how to use library automation software to collect the data. Describes the use of Microsoft Excel and its chart-making capabilities to enhance the presentation of the analysis and suggests ways to use collection analysis output. (LRW)

  7. 75 FR 62503 - Correction: Proposed Information Collection; Comment Request; Comprehensive Data Collection on...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-12

    ... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration Correction: Proposed Information Collection; Comment Request; Comprehensive Data Collection on Fishing Dependence of Alaska... collection, Comprehensive Data Collection on Fishing Dependence of Alaska Communities. Under the heading FOR...

  8. 78 FR 69095 - Agency Information Collection Activities; Proposed Collection; Comment Request; Food Canning...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-18

    ...] Agency Information Collection Activities; Proposed Collection; Comment Request; Food Canning... or we) is extending the comment period for the information collection entitled ``Food Canning... a proposed collection of information related to ``Food Canning Establishment Registration, Process...

  9. 77 FR 5306 - Agency Information Collection Activity; Proposed Collection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-02

    ... INFORMATION: Title: Adjusted Current Earnings. OMB Number: 1545-1233. Regulation Project Number: IA-14-91 (TD... OMB control number. Books or records relating to a collection of information must be retained as long... information on respondents, including through the use of automated collection techniques or other forms of...

  10. 75 FR 64354 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-19

    ... of Information Collection Under Review: ATF F 5630.5R, NFA Special Tax Renewal Registration and Return; ATF F 5630.5RC, NFA Special Tax Location Registration Listing; ATF F 5630.7, NFA Special Tax... collection. (2) Title of the Form/Collection: ATF F 5630.5R, NFA Special Tax Renewal Registration and Return...

  11. 75 FR 8408 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-24

    ...Under the Paperwork Reduction Act of 1995, Public Law 104-13 (44 U.S.C. 3501 et seq.), and as part of its continuing effort to reduce paperwork and respondent burden, the National Science Foundation (NSF) is inviting the general public or other Federal agencies to comment on this proposed continuing information collection. The NSF will publish periodic summaries of the proposed projects. Comments: Comments are invited on (a) Whether the proposed collection of information is necessary for the proper performance of the functions of the Agency, including whether the information shall have practical utility; (b) the accuracy of the Agency's estimate of the burden of the proposed collection of information; (c) ways to enhance the quality, utility, and clarity of the information to respondents, including through the use of automated collection techniques or other forms of information technology; and (d) ways to minimize the burden of the collection of information on those who are to respond, including through the use of appropriate automated, electronic, mechanical, or other technological collection techniques or other forms of information technology.

  12. Study of data collection platform concepts: Data collection system user requirements

    NASA Technical Reports Server (NTRS)

    1973-01-01

    The overall purpose of the survey was to provide real world data on user requirements. The intent was to assess data collection system user requirements by questioning actual potential users rather than speculating on requirements. The end results of the survey are baseline requirements models for both a data collection platform and a data collection system. These models were derived from the survey results. The real value of these models lies in the fact that they are based on actual user requirements as delineated in the survey questionnaires. Some users desire data collection platforms of small size and light weight. These sizes and weights are beyond the present state of the art. Also, the survey provided a wealth of information on the nature and constituency of the data collection user community as well as information on user applications for data collection systems. Finally, the data sheds light on the generalized platform concept. That is, the diversity of user requirements shown in the data indicates the difficulty that can be anticipated in attempting to implement such a concept.

  13. 78 FR 76849 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ... Activities: Proposed Collection; Comment Request; Generic Clearance for the Collection of Qualitative... Request (ICR): ``Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery... proposed information collection activity provides a means to garner qualitative customer and stakeholder...

  14. 78 FR 31578 - Agency Information Collection Activities; Proposed Collection; Comments Requested: International...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-24

    ... Collection Activities; Proposed Collection; Comments Requested: International Terrorism Victim Compensation... Form/Collection: International Terrorism Victim Expense Reimbursement Program (ITVERP) Application. (3... of acts of international terrorism that occur outside the United States. Applicants seeking...

  15. 76 FR 34231 - Agency Information Collection Activities: Submission for Review; Information Collection Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-13

    ... Information Collection. (2) Title of the Form/Collection: Science and Technology, External S&T Collaboration... DEPARTMENT OF HOMELAND SECURITY [Docket No. DHS-2011-0041] Agency Information Collection Activities: Submission for Review; Information Collection Request for the Department of Homeland Security...

  16. 76 FR 17182 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-28

    ... Activities: Proposed Collection; Comment Request; Generic Clearance for the Collection of Qualitative... Request (Generic ICR): ``Generic Clearance for the Collection of Qualitative Feedback on Agency Service...: Title: Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery. Abstract...

  17. 76 FR 20714 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-13

    ... Activities: Proposed Collection; Comment Request; Generic Clearance for the Collection of Qualitative... Collection of Qualitative Feedback on Agency Service Delivery'' to OMB for approval under the Paperwork...: Title: Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery. Abstract...

  18. 78 FR 74175 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-10

    ... precision requirements or power calculations that justify the proposed sample size, the expected response...: Proposed Collection; Comment Request; Generic Clearance for the Collection of Qualitative Feedback on... Information Collection Request (Generic ICR): ``Generic Clearance for the Collection of Qualitative Feedback...

  19. 76 FR 24920 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-03

    ... precision requirements or power calculations that justify the proposed sample size, the expected response... Collection; Comment Request; Generic Clearance for the Collection of Qualitative Feedback on Agency Service... Information Collection Request (Generic ICR): ``Generic Clearance for the Collection of Qualitative Feedback...

  20. 76 FR 19359 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-07

    ... Request; Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery AGENCY... Information Collection Request (Generic ICR): ``Generic Clearance for the Collection of Qualitative Feedback...: Title: Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery. Abstract...

  1. 77 FR 52708 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-30

    ... calculations that justify the proposed sample size, the expected response rate, methods for assessing potential...: Proposed Collection; Comment Request; Generic Clearance for the Collection of Qualitative Feedback on... Information Collection request (Generic ICR): ``Generic Clearance for the Collection of Qualitative Feedback...

  2. 77 FR 97 - Commission Information Collection Activities, Proposed Collection (FERC-598); Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-03

    ... requirements; (4) training personnel to respond to a collection of information; (5) searching data sources; (6... Information Collection Activities, Proposed Collection (FERC-598); Comment Request; Extension AGENCY: Federal Energy Regulatory Commission. ACTION: Notice of Proposed Information Collection and Request for Comments...

  3. 78 FR 2442 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Reporting and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-11

    ... Collection Activities; Proposed Collection; Comments Requested: Reporting and Recordkeeping for Digital... approved collection. (2) Title of the Form/Collection: Reporting and recordkeeping for digital certificates... forms to apply for DEA-issued digital certificates to order Schedule I and II controlled substances...

  4. 78 FR 70069 - Agency Information Collection Activities; Proposed New Collection; Comments Requested: Drug...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-22

    ... Collection; Comments Requested: Drug Endangered Children Tracking System User Survey ACTION: 60-day notice... technological collection techniques or other forms of information technology, e.g., permitting electronic... new collection; comments requested. (2) Title of the Form/Collection: Drug Endangered Children...

  5. Jay's Collectibles

    ERIC Educational Resources Information Center

    Cappel, James J.; Gillman, Jason R., Jr.

    2011-01-01

    There is growing interest in collectibles of many types, as indicated by the popularity of television programs such as the History Channel's "Pawn Stars" and "American Pickers" and the Public Broadcasting Service's "Antiques Road Show." The availability of online auction sites such as eBay has enabled many people to collect items of interest as a…

  6. 77 FR 17523 - Agency Information Collection Activities: Extension of a Currently Approved Collection; Comments...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ... Collection Activities: Extension of a Currently Approved Collection; Comments Requested; National Crime... currently approved collection. (2) Title of the form/collection: National Crime Victimization Survey. (3.... The National Crime Victimization Survey (NCVS) collects, analyzes, publishes, and disseminates...

  7. 76 FR 2395 - Agency Information Collection Activities; Proposed Collection; Comment Request; Customer/Partner...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-13

    ... comment in response to the notice. This notice solicits comments on voluntary customer satisfaction...] Agency Information Collection Activities; Proposed Collection; Comment Request; Customer/Partner Service... collection of information they conduct or sponsor. ``Collection of information'' is defined in 44 U.S.C. 3502...

  8. 77 FR 68149 - Agency Information Collection Activities; Proposed New Collection; Comments Requested: COPS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-15

    ... Collection; Comments Requested: COPS Comparative Assessment of Cost Reduction by Agencies Survey ACTION: 60... Collection: Proposed new collection; comments requested. (2) Title of the Form/Collection: COPS Comparative... Office grants or cooperative agreements will be asked complete the COPS Comparative Assessment of Cost...

  9. 76 FR 25693 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-05

    ... requirements or power calculations that justify the proposed sample size, the expected response rate, methods... Collection; Comment Request; Generic Clearance for the Collection of Qualitative Feedback on Agency Service... Collection Request (Generic ICR): ``Generic Clearance for the Collection of Qualitative Feedback on Agency...

  10. 77 FR 54610 - Agency Information Collection Activities: Proposed collection; Comments Requested: Student and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-05

    ...] Agency Information Collection Activities: Proposed collection; Comments Requested: Student and Supervisor Training Validation Surveys ACTION: 30-Day notice of information collection under review. The Department of... information collection request to the Office of Management and Budget (OMB) for review and approval in...

  11. 78 FR 52528 - Information Collection(s) Being Reviewed by the Federal Communications Commission, Comments...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-23

    ... FEDERAL COMMUNICATIONS COMMISSION Information Collection(s) Being Reviewed by the Federal... information collection(s). Comments are requested concerning: whether the proposed collection of information... information shall have practical utility; the accuracy of the Commission's burden estimate; ways to enhance...

  12. 76 FR 27352 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Dispensing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-11

    ... Collection Activities: Proposed Collection; Comments Requested: Dispensing Records of Individual.../Collection: Dispensing records of individual practitioners. (3) Agency form number, if any, and the... requires that individual practitioners keep records of the dispensing and administration of controlled...

  13. 77 FR 9952 - Agency Information Collection Activities: Proposed Collection; Comment Request, Emergency...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-21

    ... Course Evaluation Form. Type of Information Collection: Revision of a currently approved information...; OMB No. 1660-0034] Agency Information Collection Activities: Proposed Collection; Comment Request, Emergency Management Institute Course Evaluation Form AGENCY: Federal Emergency Management Agency, DHS...

  14. 75 FR 55827 - Justice Management Division; Agency Information Collection Activities: Proposed Collection...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-14

    ... DEPARTMENT OF JUSTICE [OMB Number 1103-0016] Justice Management Division; Agency Information Collection Activities: Proposed Collection; Comments Requested ACTION: 30-Day Notice of Information Collection Under Review: Certification of Identity. The Department of Justice (DOJ), Justice Management...

  15. 77 FR 47437 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Employment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-08

    ... DEPARTMENT OF JUSTICE [OMB Number 1103-NEW] Agency Information Collection Activities: Proposed Collection; Comments Requested; Employment Reference Questionnaire ACTION: 30-Day Notice of Information Collection Under Review. The Department of Justice (DOJ), Justice Management Division, Human Resources...

  16. 76 FR 5401 - Bureau of Justice Statistics; Agency Information Collection Activities: Proposed Collection...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-31

    ... collected will include the types of criminal cases appealed to state intermediate appellate courts and... notice of information collection under review: Survey of State Court Criminal Appeals, 2010. The... submission of responses. Overview of This Information (1) Type of information collection: New data collection...

  17. 78 FR 77494 - Agency Information Collection Activities; Proposed Collection; Comments Requested: ATF Adjunct...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-23

    ... collection. (2) Title of the Form/Collection: ATF Adjunct Instructor Data Form. (3) Agency form number, if...] Agency Information Collection Activities; Proposed Collection; Comments Requested: ATF Adjunct Instructor Data Form ACTION: 60-Day Notice. The Department of Justice (DOJ), Bureau of Alcohol, Tobacco, Firearms...

  18. 77 FR 1727 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Extension of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-11

    ... DEPARTMENT OF JUSTICE Office of Justice Programs [OMB Number 1121-NEW] Agency Information Collection Activities: Proposed Collection; Comments Requested; Extension of Currently Approved Collection; Bureau of Justice Assistance Application Form: National Motor Vehicle Title Information System ACTION: 60-Day Notice of Information Collection Under...

  19. 76 FR 34216 - Commission Information Collection Activities (Ferc-521), Proposed Collection; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-13

    ... Information Collection Activities (Ferc-521), Proposed Collection; Comment Request; Submitted for OMB Review... information collection described below. DATES: Comments in consideration of the collection of information are... comments and FERC issuances may be viewed, printed or downloaded remotely through FERC's eLibrary at http...

  20. 78 FR 24810 - Agency Information Collection Activities; Information Collection Renewal; Submission for OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-26

    ... Activities; Information Collection Renewal; Submission for OMB Review: Funding and Liquidity Risk Management... and Liquidity Risk Management.'' The OCC is also giving notice that the collection has been sent to... collection: Title of Information Collection: Funding and Liquidity Risk Management. OMB Control No.: 1557...

  1. 77 FR 59619 - Agency Information Collection Activities; Proposed Collection; Comment Request; Environmental...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-28

    ... appropriate, and other forms of information technology. Environmental Impact Considerations--21 CFR Part 25...] Agency Information Collection Activities; Proposed Collection; Comment Request; Environmental Impact... ``Environmental Impact Considerations.'' DATES: Submit either electronic or written comments on the collection of...

  2. 78 FR 63310 - Agency Information Collection Activities: Information Collection Renewal; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-23

    ... DEPARTMENT OF THE TREASURY Office of the Comptroller of the Currency Agency Information Collection Activities: Information Collection Renewal; Comment Request; Securities Offering Disclosure Rules AGENCY... comment concerning the renewal of an information collection titled, ``Securities Offering Disclosure Rules...

  3. 76 FR 6782 - Agency Information Collection Activities; Proposed Collection; Comment Request; Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-08

    ... ENVIRONMENTAL PROTECTION AGENCY [EPA-HQ-SFUND-2011-0052; FRL-9263-6] Agency Information Collection Activities; Proposed Collection; Comment Request; Information Collection Request for Superfund Site Evaluation and Hazard Ranking System AGENCY: Environmental Protection Agency (EPA). ACTION: Notice. SUMMARY...

  4. 77 FR 25749 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Employment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-01

    ... DEPARTMENT OF JUSTICE [OMB Number 1103-NEW] Agency Information Collection Activities: Proposed Collection; Comments Requested: Employment Reference Questionnaire ACTION: 60-Day Notice of Information Collection Under Review. The Department of Justice (DOJ), Justice Management Division (JMD), will be...

  5. 78 FR 15047 - Agency Information Collection Activities; Proposed Collection; Comments Requested: International...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-08

    ... Collection Activities; Proposed Collection; Comments Requested: International Terrorism Victim Expense.../collection: International Terrorism Victim Expense Reimbursement Program (ITVERP) Application. (3) The agency.... Government employees who are victims of acts of international terrorism that occur(red) outside of the United...

  6. 77 FR 59421 - Agency Information Collection Activities; Continued Collection; Comment Request: Vocational...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-27

    ... Activities; Continued Collection; Comment Request: Vocational Rehabilitation and Employment Tracking Report... on five (5) separate collections of information: (1) VETS 201 entitled ``Vocational Rehabilitation... particularly interested in comments on these topics: (1) Whether the continued collection of information is...

  7. 77 FR 18250 - Agency Information Collection Activities; Proposed Collection; Comment Request; Experimental...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-27

    ... of information technology. Experimental Study of Graphic Cigarette Warning Labels--(OMB Control... graphic warnings required by the Tobacco Control Act. The experimental study data will be collected from...] Agency Information Collection Activities; Proposed Collection; Comment Request; Experimental Study of...

  8. 78 FR 68092 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Certification...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-13

    ... DEPARTMENT OF JUSTICE [OMB Number 1103-0016] Agency Information Collection Activities; Proposed Collection; Comments Requested: Certification of Identity ACTION: 60-Day notice. The Department of Justice (DOJ), Justice Management Division, will be submitting the following information collection request to...

  9. 76 FR 29792 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Reports of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-23

    ... Collection Activities: Proposed Collection; Comments Requested: Reports of Suspicious Orders or Theft/Loss of...) Title of the Form/Collection: Reports of Suspicious Orders or Theft/Loss of Listed Chemicals/Machines... collection: Form number: Notification of suspicious orders and thefts is provided in writing on an as needed...

  10. 77 FR 36574 - Agency Information Collection Activities; Proposed collection, comments requested: Leased/Charter...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-19

    ... collection, comments requested: Leased/Charter Flight Personnel Expedited Clearance Request ACTION: 30-day... collection: New collection. 2. The title of the form/collection: Leased/Charter Flight Personnel Expedited.... This form is to be completed by people applying to become contract flight crew members. It is required...

  11. 77 FR 56866 - Agency Information Collection Activities: Proposed Collection; Comment Request, Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-14

    ... Collection Activities: Proposed Collection; Comment Request, Generic Clearance for Cognitive, Pilot and Field... information collection clearance that will allow BJS to conduct a variety of cognitive, pilot, and field test... new or modified data collection, BJS will engage in cognitive, pilot and field test activities to...

  12. 77 FR 56865 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-14

    ... Collection Activities: Proposed Collection; Comment Request; Generic Clearance for Cognitive, Pilot and Field... information collection clearance that will allow BJS to conduct a variety of cognitive, pilot, and field test... each new or modified data collection, BJS will engage in cognitive, pilot and field test activities to...

  13. 77 FR 56867 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-14

    ... Collection Activities: Proposed Collection; Comment Request; Generic Clearance for Cognitive, Pilot and Field... information collection clearance that will allow BJS to conduct a variety of cognitive, pilot, and field test... new or modified data collection, BJS will engage in cognitive, pilot and field test activities to...

  14. 76 FR 46325 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Red Ribbon...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-02

    ... Collection Activities: Proposed Collection; Comments Requested: Red Ribbon Week Patch DEA Form 316 and 316A... Enforcement Administration (DEA) will be submitting the following information collection request to the Office... the collection: Form number: DEA Form 316 and DEA Form 316A. Component: Office of Congressional and...

  15. 78 FR 77690 - Agency Information Collection Activities: Proposed Collection: Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-24

    ... Administration for Children and Families Agency Information Collection Activities: Proposed Collection: Public... Families, HHS. ACTION: Notice. SUMMARY: In compliance with the requirement for opportunity for public... Families (ACF) announce plans to submit an Information Collection Request (ICR), described below, to the...

  16. 78 FR 28853 - Agency Information Collection Activities; Proposed Collection; Comment Request; Postmarket...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-16

    ...] Agency Information Collection Activities; Proposed Collection; Comment Request; Postmarket Surveillance... surveillance of medical devices. DATES: Submit either electronic or written comments on the collection of... appropriate, and other forms of information technology. Postmarket Surveillance--21 CFR Part 822 (OMB Control...

  17. 75 FR 6036 - Agency Information Collection Activities; Proposed Collection; Comment Request; Postmarket...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-05

    ...] Agency Information Collection Activities; Proposed Collection; Comment Request; Postmarket Surveillance... Surveillance. DATES: Submit written or electronic comments on the collection of information by April 6, 2010... of information technology. Postmarket Surveillance--21 CFR Part 822 (OMB Control Number 0910- 0449...

  18. 76 FR 24054 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Registrants...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-29

    ... Collection; Comments Requested: Registrants Inventory of Drugs Surrendered; DEA Form 41 AGENCY: Department of...), Drug Enforcement Administration (DEA) will be submitting the following information collection request... component of the Department sponsoring the collection: Form number: DEA Form 41; component: Office of...

  19. 78 FR 54662 - Agency Information Collection Activities; Proposed Collection; Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-05

    ... information, processing and maintaining information, and disclosing and providing information; to train... Information Collection Activities; Proposed Collection; Public Comment Request AGENCY: Health Resources and... Information Collection Request (ICR), described below, to the Office of Management and Budget (OMB). Prior to...

  20. 78 FR 68448 - Agency Information Collection Activities; Proposed Collection; Public Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-14

    ... verifying information, processing and maintaining information, and disclosing and providing information, to...-60D] Agency Information Collection Activities; Proposed Collection; Public Comment Request AGENCY..., announces plans to submit a new Information Collection Request (ICR), described below, to the Office of...

  1. 77 FR 44270 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Notification...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-27

    ...] Agency Information Collection Activities; Proposed Collection; Comments Requested: Notification of Change... automated, electronic, mechanical, or other technological collection techniques or other forms of...: Notification of Change of Mailing or Premise Address. (3) Agency form number, if any, and the applicable...

  2. 78 FR 59722 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Notification...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-27

    ...] Agency Information Collection Activities: Proposed Collection; Comments Requested; Notification of Change... automated, electronic, mechanical, or other technological collection techniques or other forms of...: Notification of Change of Mailing or Premise Address. (3) Agency form number, if any, and the applicable...

  3. 78 FR 11273 - Agency Information Collection Activities; Information Collection Renewal; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-15

    ... Activities; Information Collection Renewal; Comment Request; Funding and Liquidity Risk Management AGENCY... and Liquidity Risk Management.'' DATES: Comments must be received by April 16, 2013. ADDRESSES... information collection: Title of Information Collection: Funding and Liquidity Risk Management. OMB Control No...

  4. 76 FR 10390 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Report of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    ... Collection Activities: Proposed Collection; Comments Requested: Report of Theft or Loss of Controlled...: Extension of a currently approved collection. (2) Title of the Form/Collection: Report of Theft or Loss of... complete and submit DEA-106 upon discovery of a theft or significant loss of controlled substances. This...

  5. 77 FR 1085 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Application...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-09

    ... DEPARTMENT OF JUSTICE Drug Enforcement Administration [OMB Number 1117-0047] Agency Information Collection Activities: Proposed Collection; Comments Requested: Application for Import Quota for Ephedrine, Pseudoephedrine, and Phenylpropanolamine DEA Form 488 ACTION: 30-Day Notice of Information Collection under review. The Department of Justice ...

  6. 77 FR 3003 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-20

    ... Collection; Comments Requested: Notice of Appeal From a Decision of an Immigration Judge ACTION: 30-Day...) Type of Information Collection: Revision of a Currently Approved Collection. (2) Title of the Form/Collection: Notice of Appeal from a Decision of an Immigration Judge. (3) Agency form number, if any, and the...

  7. 76 FR 72446 - Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-23

    ... OFFICE OF MANAGEMENT AND BUDGET Information Collection Activities: Proposed Collection; Comment Request AGENCY: Office of Federal Financial Management, Office of Management and Budget. ACTION: Notice....), the Office of Management and Budget (OMB) invites the general public and Federal agencies to comment...

  8. 76 FR 45299 - Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-28

    ... OFFICE OF MANAGEMENT AND BUDGET Information Collection Activities: Proposed Collection; Comment Request AGENCY: Office of Management and Budget, Office of Federal Financial Management. ACTION: Notice....), the Office of Management and Budget (OMB) invites the general public and Federal agencies to comment...

  9. 75 FR 22863 - Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-30

    ... OFFICE OF MANAGEMENT AND BUDGET Information Collection Activities: Proposed Collection; Comment Request AGENCY: Office of Management and Budget, Office of Federal Financial Management. ACTION: Notice....), the Office of Management and Budget (OMB) invites the general public and Federal agencies to comment...

  10. 76 FR 64380 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Supplemental...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-18

    ... Information on Water Quality Consideration ACTION: 30-Day Notice of Information Collection. The Department of... currently approved collection. (2) Title of the Form/Collection: Supplemental Information on Water Quality..., including the validity of the methodology and assumptions used; --Enhance the quality, utility, and clarity...

  11. 77 FR 3006 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Firearms...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-20

    ... for Nonimmigrant Aliens ACTION: 30-Day notice of information collection. The Department of Justice...) Title of the Form/Collection: Firearms Disabilities for Nonimmigrant Aliens. (3) Agency form number, if... for Collection The nonimmigrant alien information will be used to determine if a nonimmigrant alien is...

  12. 77 FR 57590 - Agency Information Collection Activities; Proposed Collection; Comments Requested: ATF...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-18

    ... form is used to evaluate the ATF Distribution Center and the services it provides to the users of ATF...] Agency Information Collection Activities; Proposed Collection; Comments Requested: ATF Distribution Center Survey ACTION: 60-Day notice of information collection under review. The Department of Justice...

  13. 76 FR 24057 - Agency Information Collection Activities; Proposed Collection; Comments Requested; ARCOS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-29

    ... Collection; Comments Requested; ARCOS Transaction Reporting DEA Form 333 ACTION: 30-Day Notice of Information Collection Under Review. The Department of Justice (DOJ), Drug Enforcement Administration (DEA) will be... applicable component of the Department of Justice sponsoring the collection: Form Number: DEA Form 333...

  14. 78 FR 31557 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-24

    ..., and healthcare practitioners. This information collection request explains the research methodology and data collection strategies designed to minimize the burden placed on research participants, while...

  15. 77 FR 26045 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Certification...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-02

    ... Secure Gun Storage or Safety Devices ACTION: 30-Day Notice of information collection. The Department of... approved collection. (2) Title of the Form/Collection: Certification of Secure Gun Storage or Safety... to the availability of secure gun storage or safety devices. (5) An estimate of the total number of...

  16. Collecting verbal autopsies: improving and streamlining data collection processes using electronic tablets.

    PubMed

    Flaxman, Abraham D; Stewart, Andrea; Joseph, Jonathan C; Alam, Nurul; Alam, Sayed Saidul; Chowdhury, Hafizur; Mooney, Meghan D; Rampatige, Rasika; Remolador, Hazel; Sanvictores, Diozele; Serina, Peter T; Streatfield, Peter Kim; Tallo, Veronica; Murray, Christopher J L; Hernandez, Bernardo; Lopez, Alan D; Riley, Ian Douglas

    2018-02-01

    There is increasing interest in using verbal autopsy to produce nationally representative population-level estimates of causes of death. However, the burden of processing a large quantity of surveys collected with paper and pencil has been a barrier to scaling up verbal autopsy surveillance. Direct electronic data capture has been used in other large-scale surveys and can be used in verbal autopsy as well, to reduce time and cost of going from collected data to actionable information. We collected verbal autopsy interviews using paper and pencil and using electronic tablets at two sites, and measured the cost and time required to process the surveys for analysis. From these cost and time data, we extrapolated costs associated with conducting large-scale surveillance with verbal autopsy. We found that the median time between data collection and data entry for surveys collected on paper and pencil was approximately 3 months. For surveys collected on electronic tablets, this was less than 2 days. For small-scale surveys, we found that the upfront costs of purchasing electronic tablets was the primary cost and resulted in a higher total cost. For large-scale surveys, the costs associated with data entry exceeded the cost of the tablets, so electronic data capture provides both a quicker and cheaper method of data collection. As countries increase verbal autopsy surveillance, it is important to consider the best way to design sustainable systems for data collection. Electronic data capture has the potential to greatly reduce the time and costs associated with data collection. For long-term, large-scale surveillance required by national vital statistical systems, electronic data capture reduces costs and allows data to be available sooner.

  17. 77 FR 25751 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Certificate of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-01

    ... Compliance with 18 U.S.C. 922(g)(5)(B) ACTION: Correction 30-Day Notice of Information Collection. This is to... currently approved collection. (2) Title of the Form/Collection: Certificate of Compliance with 18 U.S.C...: Business or other for-profit. Other: None. Need for Collection The law of 18 U.S.C. 922(g)(5)(B) makes it...

  18. 75 FR 80847 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-23

    ... of Information Collection Under Review: Implementation of Public Law 103-322, The Violent Crime... Form/Collection: Implementation of Public Law 103- 322, The Violent Crime Control and Law Enforcement...

  19. 76 FR 20042 - Agency Information Collection Activities: Proposed Collection, Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-11

    ... Collection Under Review: Revision of a currently approved collection; Supplementary Homicide Report. [[Page...: Supplementary Homicide Report. (3) The agency form number, if any, and the applicable component of the...

  20. 75 FR 44815 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-29

    ... title of the information collection: NRC Form 4, ``Cumulative Occupational Dose History''. 2. Current... minimized, including the use of automated collection techniques or other forms of information technology? A...

  1. 77 FR 8277 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-14

    ... approved collection. (2) Title of the Form/Collection: Methodological research to support the redesign of... to take 15-30 minutes, while a cognitive interview for testing alternative methods for measuring...

  2. Construction of the first genetic linkage map of Japanese gentian (Gentianaceae)

    PubMed Central

    2012-01-01

    Background Japanese gentians (Gentiana triflora and Gentiana scabra) are amongst the most popular floricultural plants in Japan. However, genomic resources for Japanese gentians have not yet been developed, mainly because of the heterozygous genome structure conserved by outcrossing, the long juvenile period, and limited knowledge about the inheritance of important traits. In this study, we developed a genetic linkage map to improve breeding programs of Japanese gentians. Results Enriched simple sequence repeat (SSR) libraries from a G. triflora double haploid line yielded almost 20,000 clones using 454 pyrosequencing technology, 6.7% of which could be used to design SSR markers. To increase the number of molecular markers, we identified three putative long terminal repeat (LTR) sequences using the recently developed inter-primer binding site (iPBS) method. We also developed retrotransposon microsatellite amplified polymorphism (REMAP) markers combining retrotransposon and inter-simple sequence repeat (ISSR) markers. In addition to SSR and REMAP markers, modified amplified fragment length polymorphism (AFLP) and random amplification polymorphic DNA (RAPD) markers were developed. Using 93 BC1 progeny from G. scabra backcrossed with a G. triflora double haploid line, 19 linkage groups were constructed with a total of 263 markers (97 SSR, 97 AFLP, 39 RAPD, and 30 REMAP markers). One phenotypic trait (stem color) and 10 functional markers related to genes controlling flower color, flowering time and cold tolerance were assigned to the linkage map, confirming its utility. Conclusions This is the first reported genetic linkage map for Japanese gentians and for any species belonging to the family Gentianaceae. As demonstrated by mapping of functional markers and the stem color trait, our results will help to explain the genetic basis of agronomic important traits, and will be useful for marker-assisted selection in gentian breeding programs. Our map will also be an

  3. Portable liquid collection electrostatic precipitator

    DOEpatents

    Carlson, Duane C.; DeGange, John J.; Halverson, Justin E.

    2005-10-18

    A portable liquid collection electrostatic collection precipitator for analyzing air is provided which is a relatively small, self-contained device. The device has a tubular collection electrode, a reservoir for a liquid, and a pump. The pump pumps the liquid into the collection electrode such that the liquid flows down the exterior of the collection electrode and is recirculated to the reservoir. An air intake is provided such that air to be analyzed flows through an ionization section to ionize analytes in the air, and then flows near the collection electrode where ionized analytes are collected. A portable power source is connected to the air intake and the collection electrode. Ionizable constituents in the air are ionized, attracted to the collection electrode, and precipitated in the liquid. The precipitator may also have an analyzer for the liquid and may have a transceiver allowing remote operation and data collection.

  4. 76 FR 32379 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-06

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2011-0114] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission (NRC). ACTION: Notice of pending...: Applications for licenses are [[Page 32380

  5. Satellite image collection optimization

    NASA Astrophysics Data System (ADS)

    Martin, William

    2002-09-01

    Imaging satellite systems represent a high capital cost. Optimizing the collection of images is critical for both satisfying customer orders and building a sustainable satellite operations business. We describe the functions of an operational, multivariable, time dynamic optimization system that maximizes the daily collection of satellite images. A graphical user interface allows the operator to quickly see the results of what if adjustments to an image collection plan. Used for both long range planning and daily collection scheduling of Space Imaging's IKONOS satellite, the satellite control and tasking (SCT) software allows collection commands to be altered up to 10 min before upload to the satellite.

  6. Flowing together: a longitudinal study of collective efficacy and collective flow among workgroups.

    PubMed

    Salanova, Marisa; Rodríguez-Sánchez, Alma M; Schaufeli, Wilmar B; Cifre, Eva

    2014-01-01

    The aim of this study is to extend the Channel Model of Flow (Csikszentmihalyi, 1975, 1990) at the collective level (workgroups) by including collective efficacy beliefs as a predictor of collective flow based on the Social Cognitive Theory (Bandura, 1997, 2001). A two-wave longitudinal lab study was conducted with 250 participants working in 52 small groups. Longitudinal results from Structural Equation Modeling with data aggregated at the group level showed, as expected, that collective efficacy beliefs predict collective flow over time, both being related reciprocally. Findings and their theoretical and practical implications in the light of Social Cognitive Theory are discussed.

  7. Nebraska data collection.

    DOT National Transportation Integrated Search

    2015-12-01

    Automated pavement performance data collection is a method that uses advanced technology to collect detailed road surface : distress information at traffic speed. Agencies are driven to use automated survey techniques to enhance or replace their : cu...

  8. 77 FR 55475 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-10

    ... collected will be analyzed to produce estimates and basic descriptive statistics on the quantity and type of... mode of data collection by event types, and conduct correlations, cross tabulations of responses and...

  9. 77 FR 35981 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-15

    ... collected; and (4) the use of automated collection techniques or other forms of information technology to... options and help them assess the advantages and disadvantages each choice holds for them. The Medicare...

  10. 77 FR 43092 - Agency Information Collection Activities; Proposed Collection; Comment Request; Chronic Disease...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-23

    ... Collection Activities; Proposed Collection; Comment Request; Chronic Disease Self-Management Education... through Chronic Disease Self-Management Education (CDSME) Programs'' cooperative agreement program is... solicits comments on the information collection requirements relating to the Chronic Disease Self...

  11. 77 FR 75117 - Agency Information Collection Activities: Proposed Collection; Comment Request-Federal Claims...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-19

    ... DEPARTMENT OF AGRICULTURE Food and Nutrition Service Agency Information Collection Activities: Proposed Collection; Comment Request--Federal Claims Collection Methods for Supplemental Nutrition Assistance Program Recipient Claims AGENCY: Food and Nutrition Service, USDA. ACTION: Notice. SUMMARY: In...

  12. 22 CFR 309.11 - Collection.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Collection. 309.11 Section 309.11 Foreign Relations PEACE CORPS DEBT COLLECTION Collection Actions § 309.11 Collection. Upon final determination of.... Collection may be through centralized offset by the Financial Management Service (FMS) of the Department of...

  13. 78 FR 1249 - Agency Information Collection Activities; Extension of a Currently Approved Collection; Comment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-08

    ... a Currently Approved Collection; Comment Request: Equal Employment Opportunity Plan Certification... Collection: Revision of a currently approved collection. (2) Title of the Form/Collection: Equal Employment... hour to complete an Equal Employment Opportunity Plan Short Form and submit it to the Office of Justice...

  14. 75 FR 4613 - Agency Information Collection Activities: Request for Comments for New Information Collection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-28

    ... Information Collection Activities: Request for Comments for New Information Collection AGENCY: Federal Highway... information collection, which is summarized below under SUPPLEMENTARY INFORMATION. We published a Federal Register Notice with a 60-day public comment period on this information collection on November 5, 2009. We...

  15. 77 FR 69658 - Agency Information Collection Activities: Revision of a Currently Approved Collection: Annuity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-20

    ... a Currently Approved Collection: Annuity Broker Qualification Declaration Form ACTION: 30-Day Notice... Collection: Revision of a currently approved collection. (2) Title of the Form/Collection: Annuity Broker... submitted annually to determine whether a broker meets the qualifications to be listed as an annuity broker...

  16. 78 FR 76153 - Agency Information Collection Activities: Comment Request; Extension of an Information Collection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-16

    ... Notice of Information Collection for review; Electronic Bonds Online (eBonds) Access; OMB Control No... submitting the following information collection request for review and clearance in accordance [[Page 76154... information collection. (2) Title of the Form/Collection: Electronic Bonds Online (eBonds) Access. (3) Agency...

  17. 77 FR 15391 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Extension of a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-15

    ... DEPARTMENT OF JUSTICE [OMB Number 1121-0335] Agency Information Collection Activities: Proposed Collection; Comments Requested; Extension of a Currently Approved Collection; Bureau of Justice Assistance: National Motor Vehicle Title Information System ACTION: 30-Day Notice of Information Collection Under Review. The Department of Justice, Office...

  18. 76 FR 56224 - Agency Information Collection Activities: Proposed Collection, Comments Requested: Revision of a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-12

    ... DEPARTMENT OF JUSTICE Federal Bureau of Investigation [OMB Number 1110-0015] Agency Information Collection Activities: Proposed Collection, Comments Requested: Revision of a Currently Approved Collection: Hate Crime Incident Report; Quarterly Hate Crime Report ACTION: 30-day Notice of Information Collection under Review. [[Page 56225

  19. 75 FR 31787 - Agency Information Collection Activities: Existing Collection; Emergency Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-04

    ... EQUAL EMPLOYMENT OPPORTUNITY COMMISSION Agency Information Collection Activities: Existing Collection; Emergency Extension AGENCY: Equal Employment Opportunity Commission. ACTION: Notice of... Paperwork Reduction Act, the Equal Employment Opportunity Commission (EEOC or Commission) announces that it...

  20. 32 CFR 204.6 - Collections.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Collections. 204.6 Section 204.6 National... USER FEES § 204.6 Collections. (a) Collections of fees will be made in advance or simultaneously with..., and managing cash and debt collections. (b) Unless a statute provides otherwise, user fee collections...

  1. 25 CFR 175.32 - Collections.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Collections. 175.32 Section 175.32 Indians BUREAU OF..., Payments, and Collections § 175.32 Collections. The utility shall attempt collection on checks returned by... for each collection action taken by the utility other than court proceedings. An unredeemed check...

  2. 5 CFR 1215.25 - Collection.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Collection. 1215.25 Section 1215.25... Collection § 1215.25 Collection. (a) The MSPB will take aggressive action to collect debts and reduce delinquencies. Collection efforts shall include sending to the debtor's last known address a total of three...

  3. 77 FR 16516 - Agency Information Collection Activities: Proposed Collection; Comment Request-Special Milk...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-21

    ... DEPARTMENT OF AGRICULTURE Food and Nutrition Service Agency Information Collection Activities: Proposed Collection; Comment Request--Special Milk Program for Children AGENCY: Food and Nutrition Service... appropriate automated, electronic, mechanical, or other technological collection techniques or other forms of...

  4. 76 FR 50993 - Agency Information Collection Activities: Proposed Collection; Comment Request-Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-17

    ...: Proposed Collection; Comment Request--Generic Clearance to Conduct Methodological Testing, Surveys, Focus... proposed information collection. This information collection will conduct research by methodological... Methodological Testing, Surveys, Focus Groups, and Related Tools to Improve the Management of Federal Nutrition...

  5. 78 FR 32275 - Agency Information Collection Activities; Proposed Collection; Comments Requested; Extension of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-29

    ... DEPARTMENT OF JUSTICE [OMB Number 1121-0220] Agency Information Collection Activities; Proposed Collection; Comments Requested; Extension of Currently Approved Collection: Bureau of Justice Assistance Application Form: Public Safety Officers' Educational Assistance ACTION: 30-Day Notice. The Department of...

  6. 78 FR 59095 - Agency Information Collection Activities: Information Collection Renewal; Submission for OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-25

    ... Activities: Information Collection Renewal; Submission for OMB Review; Guidance on Sound Incentive... concerning renewal of an information collection titled, ``Guidance on Sound Incentive Compensation Practices... following collection: Title: Guidance on Sound Incentive Compensation Policies. OMB Number: 1557-0245...

  7. 78 FR 1250 - Agency Information Collection Activities: Proposed New Collection; Comments Requested; Stress...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-08

    ... Collection; Comments Requested; Stress Resiliency Study Questionnaires for Milwaukee Police Department ACTION... new collection; comments requested. (2) Title of the Form/Collection: Stress Resiliency Study... MPD officers' perceived stress, responses to stressful experiences, stress and its relationship to...

  8. 77 FR 29352 - Agency Information Collection Activities; Proposed Collection; Comment Request; Irradiation in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-17

    ...] Agency Information Collection Activities; Proposed Collection; Comment Request; Irradiation in the... information collection provisions of FDA's requirements for food irradiation processors. DATES: Submit either... of information technology. Irradiation in the Production, Processing, and Handling of Food--21 CFR...

  9. Collective and non-collective structures in nuclei of mass region A ≈ 125

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Singh, A. K.; Collaboration: INGA Collaboration; Gammasphere Collaboration

    Generation of angular momentum in nuclei is a key question in nuclear structure studies. In single particle model, it is due to alignment of spin of individual nucleon available in the valence space, whereas coherent motion of nucleons are assumed in the collective model. The nuclei near the closed shell at Z = 50 with mass number A ≈ 120-125 represent ideal cases to explore the interplay between these competing mechanisms and the transition from non-collective to collective behavior or vice versa. Recent spectroscopic studies of nuclei in this region reveal several non-collective maximally aligned states representing the first kindmore » of excitation mechanism, where 8-12 particles above the {sup 114}Sn align their spins to generate these states. Deformed rotational bands feeding the non-collective states in the spin range I=20-25 and excitation energies around 10 MeV have also been observed. Structure of the collective and non-collective states are discussed in the framework of Cranked-Nilsson-Strutinsky model.« less

  10. 77 FR 22345 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Body Armor in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-13

    ... correctional officers, such as the proportion who have body armor, the rate of use, the decision-making factors... Collection; Comments Requested; Body Armor in Correctional Institutions Survey ACTION: 60-day notice of... Collection: Establishment survey and initial approval of collection. (2) Title of Form/Collection: Body Armor...

  11. 77 FR 75660 - Agency Information Collection Activities; Proposed Collection; Comments Requested: FEL Out-of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-21

    ...] Agency Information Collection Activities; Proposed Collection; Comments Requested: FEL Out-of-Business.... (2) Title of the Form/Collection: FEL Out of Business Records. (3) Form Number: None. Bureau of..., as well as a brief abstract: Primary: Business or other for-profit. Other: None. Need for Collection...

  12. 76 FR 39437 - Agency Information Collection Activities: Proposed Collection, Comments Requested Revision of a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-06

    ... DEPARTMENT OF JUSTICE Federal Bureau of Investigation [OMB Number 1110-0015] Agency Information Collection Activities: Proposed Collection, Comments Requested Revision of a Currently Approved Collection; Hate Crime Incident Report; Quarterly Hate Crime Report ACTION: 60-day Notice of Information Collection Under Review. The Department of Justice...

  13. 78 FR 33153 - Agency Information Collection Activities; Information Collection Renewal; Submission for OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-03

    ...The OCC, as part of its continuing effort to reduce paperwork and respondent burden, invites the general public and other Federal agencies to comment on a continuing information collection, as required by the Paperwork Reduction Act of 1995 (PRA). Under PRA, Federal agencies are required to publish notice in the Federal Register concerning each proposed collection of information, including each proposed extension of an existing collection of information. In accordance with the requirements of the PRA, the OCC may not conduct or sponsor, and the respondent is not required to respond to, an information collection unless it displays a currently valid Office of Management and Budget (OMB) control number. The OCC is soliciting comment concerning its information collection titled, ``Notice Regarding Unauthorized Access to Customer Information.'' The OCC is also giving notice that it has sent the collection to OMB for review.

  14. 76 FR 7591 - Agency Information Collection Activities: Proposed Collection, Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-10

    ... investments in research, development, infrastructure, and capacity-building for science, technology... informal science community with new expectations for rigorous research on implementation and outcomes. The... NATIONAL SCIENCE FOUNDATION Agency Information Collection Activities: Proposed Collection, Comment...

  15. Blood Collection

    NASA Technical Reports Server (NTRS)

    1999-01-01

    The method that is used for the collection, storage and real-time analysis of blood and other bodily fluids has been licensed to DBCD, Inc. by NASA. The result of this patent licensing agreement has been the development of a commercial product that can provide serum or plasma from whole blood volumes of 20 microliters to 4 milliliters. The device has a fibrous filter with a pore size of less than about 3 microns, and is coated with a mixture of mannitol and plasma fraction protein. The coating causes the cellular fraction to be trapped by the small pores, leaving the cellular fraction intact on the fibrous filter while the acellular fraction passes through the filter for collection in unaltered form from the serum sample collection chamber. The method used by this product is useful to NASA for blood analysis on manned space missions.

  16. 12 CFR 313.22 - Collection.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Collection. 313.22 Section 313.22 Banks and... DEBT COLLECTION Administrative Offset § 313.22 Collection. (a) The Director may collect a claim from a... complied with this part. (b) Prior to initiating collection by administrative offset, the Director should...

  17. 76 FR 34079 - Agency Information Collection Activities; Proposed Collection; Comment Request; Regulations for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-10

    ...] Agency Information Collection Activities; Proposed Collection; Comment Request; Regulations for In Vivo Radiopharmaceuticals Used for Diagnosis and Monitoring AGENCY: Food and Drug Administration, HHS. ACTION: Notice... collection for in vivo Radiopharmaceuticals Used for Diagnosis and Monitoring. DATES: Submit either...

  18. 78 FR 22946 - Agency Information Collection Activities; Information Collection Renewal; Submission for OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-17

    ... DEPARTMENT OF THE TREASURY Office of the Comptroller of the Currency Agency Information Collection Activities; Information Collection Renewal; Submission for OMB Review: Municipal Securities Dealers and... proposing to extend OMB approval of the following information collection: Title: Municipal Securities...

  19. 75 FR 77645 - Agency Information Collection Activities; Proposed Collection; Comment Request; Color Additive...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-13

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2010-N-0622] Agency Information Collection Activities; Proposed Collection; Comment Request; Color Additive... collection provisions of FDA's regulations governing batch certification of color additives manufactured for...

  20. 76 FR 64092 - Agency Information Collection Activities: Proposed Collection; Comment Request, Title: Public...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-17

    ..., Title: Public Assistance Customer Satisfaction Survey AGENCY: Federal Emergency Management Agency, DHS.... Collection of Information Title: Public Assistance Customer Satisfaction Survey. Type of Information Collection: Revision of a currently approved information collection. OMB Number: 1660-0107. Form Titles and...

  1. 78 FR 70577 - Agency Information Collection Activities; Proposed Collection, Comments Requested, New Collection...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-26

    ... Program the ability to conduct pretests which evaluate the validity and reliability of information... the proposed collection of information, including the validity of the methodology and assumptions used...

  2. 77 FR 56860 - Agency Information Collection Activities; Extension of a Currently Approved Collection: Annuity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-14

    ... of a Currently Approved Collection: Annuity Broker Declaration Form ACTION: 60-Day notice of... Collection: Revision of a currently approved collection. (2) Title of the Form/Collection: Annuity Broker... annuity broker pursuant to Section 111015(b) of Public Law 107-273. (5) An estimate of the total number of...

  3. 78 FR 36780 - Agency Information Collection Activities: Proposed Collection Renewals; Comment Request Re...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-19

    ... Collection Renewals; Comment Request Re: Insurance Sales Consumer Protections AGENCY: Federal Deposit... is seeking comment on renewal of its Insurance Sales Consumer Protections information collection (OMB... collections of information: Title: Insurance Sales Consumer Protections. OMB Number: 3064-0140. Frequency of...

  4. 76 FR 41264 - Agency Information Collection Activities; Proposed Collection; Comment Request; Appeals of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-13

    ...] Agency Information Collection Activities; Proposed Collection; Comment Request; Appeals of Science-Based... notice solicits comments on the information collection requirements for appeals of science-based... appropriate, and other forms of information technology. Appeals of Science-Based Decisions Above the Division...

  5. 78 FR 62632 - Agency Information Collection Activities; Proposed Collection Renewal; Comment Request Re...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-22

    ... Collection Renewal; Comment Request Re: Guidance on Sound Incentive Compensation Practices AGENCY: Federal... notice that it is seeking comment on renewal of its information collection, entitled Guidance on Sound... the following currently approved collections of information: Title: Guidance on Sound Incentive...

  6. 76 FR 35069 - Agency Information Collection Activities; Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-15

    ... precision requirements or power calculations that justify the proposed sample size, the expected response...; Proposed Collection; Comment Request; Generic Clearance for the Collection of Qualitative Feedback on... (Generic ICR): ``Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery...

  7. 78 FR 41065 - Agency Information Collection Activities; Proposed Collection; Comment Request; Medical Devices...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-09

    ...] Agency Information Collection Activities; Proposed Collection; Comment Request; Medical Devices; Third... notice solicits comments on the information collection associated with medical devices third-party review... that members of the public submit reports, keep records, or provide information to a third party...

  8. 76 FR 45573 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-29

    ... Technology Research Center survey. Abstract: The information collection activity will garner qualitative.... This type of generic clearance for qualitative information will not be used for quantitative... Collection; Comment Request; Generic Clearance for the Collection of Qualitative Feedback on Agency Service...

  9. Guide to data collection

    NASA Technical Reports Server (NTRS)

    1981-01-01

    Guidelines and recommendations are presented for the collection of software development data. Motivation and planning for, and implementation and management of, a data collection effort are discussed. Topics covered include types, sources, and availability of data; methods and costs of data collection; types of analyses supported; and warnings and suggestions based on software engineering laboratory (SEL) experiences. This document is intended as a practical guide for software managers and engineers, abstracted and generalized from 5 years of SEL data collection.

  10. 77 FR 36297 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-18

    ... NATIONAL ARCHIVES AND RECORDS ADMINISTRATION Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: National Archives and Records Administration (NARA). ACTION: Notice... Reduction Act Comments (NHP), Room 4400, National Archives and Records Administration, 8601 Adelphi Rd...

  11. 78 FR 78401 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-26

    ... NATIONAL ARCHIVES AND RECORDS ADMINISTRATION [NARA-2014-012] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: National Archives and Records Administration (NARA... biomedical statistical research in archival records containing highly personal information. The second is...

  12. 76 FR 72449 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-23

    ... NATIONAL ARCHIVES AND RECORDS ADMINISTRATION Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: National Archives and Records Administration (NARA). ACTION: Notice... Reduction Act Comments (ISP), Room 4400, National Archives and Records Administration, 8601 Adelphi Rd...

  13. 75 FR 52992 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-30

    ... NATIONAL ARCHIVES AND RECORDS ADMINISTRATION Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: National Archives and Records Administration (NARA). ACTION: Notice... records matter. The information will support adjustments in this offering that will improve the overall...

  14. 75 FR 69474 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-12

    ... NATIONAL ARCHIVES AND RECORDS ADMINISTRATION Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: National Archives and Records Administration (NARA). ACTION: Notice... research in archival records containing highly personal information. The second is an application that is...

  15. 77 FR 50532 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-21

    ... NATIONAL ARCHIVES AND RECORDS ADMINISTRATION Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: National Archives and Records Administration (NARA). ACTION: Notice... Personnel Folders or Employee Medical Folders from the National Personnel Records Center (NPRC) of the...

  16. 75 FR 66802 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-29

    ... NATIONAL ARCHIVES AND RECORDS ADMINISTRATION Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: National Archives and Records Administration (NARA). ACTION: Notice...: NATF 81, National Archives Order for Copies of Ship Passenger Arrival Records; NATF 82, National...

  17. 76 FR 46855 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... NATIONAL ARCHIVES AND RECORDS ADMINISTRATION Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: National Archives and Records Administration (NARA). ACTION: Notice... original archival records in a National Archives and Records Administration facility. The public is invited...

  18. 76 FR 64382 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-18

    ... Collection Under Review: Office for Victims of Crime Trafficking Information Management System (TIMS). The... following information collection request to the Office of Management and Budget (OMB) for review and... Trafficking Information Management System (TIMS) Online. [[Page 64383

  19. 78 FR 24811 - Agency Information Collection Activities; Proposed Information Collection; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-26

    ...The OCC, as part of its continuing effort to reduce paperwork and respondent burden, invites the general public and other Federal agencies to comment on a continuing information collection, as required by the Paperwork Reduction Act of 1995. Under the Paperwork Reduction Act of 1995 (PRA), Federal agencies are required to publish notice in the Federal Register concerning each proposed collection of information, including each proposed extension of an existing collection of information and to allow 60 days for public comment in response to the notice. In accordance with the requirements of the PRA, the OCC may not conduct or sponsor, and the respondent is not required to respond to, an information collection unless it displays a currently valid Office of Management and Budget (OMB) control number. The OCC is soliciting comment concerning renewal of its information collection titled, ``Interagency Guidance on Asset Securitization Activities.''

  20. 78 FR 70951 - Agency Information Collection Activities; Proposed Collection; Comment Request; Importer's Entry...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-27

    ... written comments on the collection of information to the Division of Dockets Management (HFA- 305), Food... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2013-N-1423] Agency Information Collection Activities; Proposed Collection; Comment Request; Importer's Entry Notice...

  1. 76 FR 38450 - 60-Day Notice of Proposed Information Collections: Ten Information Collections

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-30

    ... DEPARTMENT OF STATE [Public Notice: 7512] 60-Day Notice of Proposed Information Collections: Ten Information Collections ACTION: Notice of request for public comments. SUMMARY: The Department of State is seeking Office of Management and Budget (OMB) approval for the information collections described below...

  2. 77 FR 10558 - Agency Information Collection Activities; Proposed Collection; Comments Requested: Request for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-22

    ..., or Similar Organization (Form EOIR-31) ACTION: 60-Day notice of information collection under review... DEPARTMENT OF JUSTICE [OMB Number 1125-0012] Agency Information Collection Activities; Proposed... following information collection request to the Office of Management and Budget (OMB) for review and...

  3. 77 FR 25196 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Request for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-27

    ..., or Similar Organization (Form EOIR-31) ACTION: 30-Day notice of information collection under review... DEPARTMENT OF JUSTICE [OMB Number 1125-0012] Agency Information Collection Activities: Proposed... following information collection request to the Office of Management and Budget (OMB) for review and...

  4. 76 FR 81966 - Agency Information Collection Activities; Proposed Collection; Comments Requested; Assumption of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-29

    ... Indian Country ACTION: 60-Day notice of information collection under review. The Department of Justice, Office of Tribal Justice, will be submitting the following information collection request to the Office... DEPARTMENT OF JUSTICE [OMB Number 1105-0091] Agency Information Collection Activities; Proposed...

  5. 76 FR 61360 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-04

    ... calculations that justify the proposed sample size, the expected response rate, methods for assessing potential... Activities: Proposed Collection; Comment Request; Generic Clearance for the Collection of Qualitative... Collection of Qualitative Feedback on Agency Service Delivery'' to OMB for approval under the Paperwork...

  6. 76 FR 55078 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-06

    ...; Comment Request; Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery...): ``Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery'' to OMB for.... SUPPLEMENTARY INFORMATION: Title: Generic Clearance for the Collection of Qualitative Feedback on Agency Service...

  7. 76 FR 22937 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-25

    ...; Comment Request; Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery... the Collection of Qualitative Feedback on Agency Service Delivery '' to OMB for approval under the... INFORMATION: Title: Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery...

  8. 76 FR 39893 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-07

    ...; Comment Request; Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery... (Generic ICR): ``Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery.... SUPPLEMENTARY INFORMATION: Title: Generic Clearance for the Collection of Qualitative Feedback on Agency Service...

  9. 76 FR 13338 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-11

    ... Collection of Qualitative Feedback on Agency Service Delivery AGENCY: Administrative Conference of the United... Request (Generic ICR): ``Generic Clearance for the Collection of Qualitative Feedback on Agency Service.... SUPPLEMENTARY INFORMATION: Title: Generic Clearance for the Collection of Qualitative Feedback on Agency Service...

  10. 76 FR 17861 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-31

    ... requirements or power calculations that justify the proposed sample size, the expected response rate, methods...; Comment Request; Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery... Information Collection Request (Generic ICR): ``Generic Clearance for the Collection of Qualitative Feedback...

  11. 76 FR 40981 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-12

    ...: Proposed Collection; Comment Request; Generic Clearance for the Collection of Qualitative Feedback on... Qualitative Feedback on Agency Service Delivery '' to OMB for approval under the Paperwork Reduction Act (PRA... 20590. SUPPLEMENTARY INFORMATION: Title: Generic Clearance for the Collection of Qualitative Feedback on...

  12. 75 FR 80542 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-22

    ... calculations that justify the proposed sample size, the expected response rate, methods for assessing potential...; Comment Request; Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery... Collection Request (Generic ICR): ``Generic Clearance for the Collection of Qualitative Feedback on Agency...

  13. 76 FR 41280 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-13

    ... proposed sample size, the expected response rate, methods for assessing potential non-response bias, the... Activities: Proposed Collection; Comment Request; Generic Clearance for the Collection of Qualitative...): ``Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery '' to OMB for...

  14. 78 FR 4414 - Agency Information Collection Activities; Proposed Collection; Comment Request; Registration of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-22

    ... affected by a deliberate or accidental contamination of the food supply. In addition, data collected... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2013-N-0065] Agency Information Collection Activities; Proposed Collection; Comment Request; Registration of Food...

  15. 76 FR 44904 - Agency Information Collection Activities; Proposed Collection; Comment Request; State Water...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-27

    ... ENVIRONMENTAL PROTECTION AGENCY [EPA-HQ-OW-2011-0424; FRL-9443-7] Agency Information Collection Activities; Proposed Collection; Comment Request; State Water Quality Program Management Resource Analysis... is planning to submit a request for a new Information Collection Request (ICR) to the Office of...

  16. 77 FR 75110 - Agency Information Collection Activities: Proposed Collection; Comment Request-Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-19

    ...: Proposed Collection; Comment Request--Generic Clearance To Conduct Formative Research AGENCY: Food and.... This information collection will conduct research in support of FNS' goal of delivering science-based... target audience, FNS plans to conduct data collections that involve formative research including focus...

  17. 75 FR 30783 - Agency Information Collection Activities; Proposed Collection; Comment Request; Safety Standard...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-02

    ... CONSUMER PRODUCT SAFETY COMMISSION [Docket No. CPSC-2009-0064] Agency Information Collection... announcing an opportunity for public comment on the proposed collection of certain information by the agency... the Federal Register concerning each proposed collection of information, and to allow 60 days for...

  18. 77 FR 6827 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-09

    ... NUCLEAR REGULATORY COMMISSION [NRC-2011-0280] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC action to... public comment. SUMMARY: The Nuclear Regulatory Commission (NRC) invites public comment about our...

  19. 76 FR 28791 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-18

    ... participation for expenditures under their Medicaid Electronic Health Record Incentive Program related to health... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Medicare & Medicaid Services [Document... currently approved collection; Title of Information Collection: State Medicaid Health Information Technology...

  20. 78 FR 5807 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-28

    ... employ the complementary quantitative and qualitative methods of previous research. The combination of... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research...

  1. 78 FR 953 - Agency Information Collection Activities: Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-07

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Health Resources and Services Administration Agency Information Collection Activities: Proposed Collection: Comment Request ACTION: Notice. Summary: In compliance.... L. 104-13), the Health Resources and Services Administration (HRSA) publishes periodic summaries of...

  2. 77 FR 65204 - Agency Information Collection Activities; Proposed Collection; Comment Request: Equal Employment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-25

    ... Collection; Comment Request: Equal Employment Opportunity Plan Certification of Compliance and Short Form... collection. (2) Title of the Form/Collection: Certification Form: Compliance with the Equal Employment... grant of $500,000 or more four hours to complete the Equal Employment Opportunity Plan Short Form and...

  3. 75 FR 4594 - Proposed Data Collection(s) Available for Public Comment and Recommendations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-28

    ... surveys designed to determine the kinds and quality of services our beneficiaries, claimants, employers... of Information Collection Voluntary Customer Surveys in Accordance with E.O. 12862; OMB 3220-0192 In... surveys are limited to data collections that solicit strictly voluntary opinions, and do not collect...

  4. 77 FR 32087 - Agency Information Collection Activities; Proposed Collection; Comment Request; Participation by...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-31

    ... ENVIRONMENTAL PROTECTION AGENCY [EPA-HQ-OA-2006-0278; FRL-9669-9] Agency Information Collection... submit a request to reinstate an Information Collection Request (ICR) to the Office of Management and... approval, EPA is soliciting comments on specific aspects of the proposed information collection as...

  5. 75 FR 79056 - Proposed Data Collection(s) Available for Public Comment and Recommendations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-17

    ... RAILROAD RETIREMENT BOARD Proposed Data Collection(s) Available for Public Comment and Recommendations SUMMARY: In accordance with the requirement of Section 3506 (c)(2)(A) of the Paperwork Reduction Act of 1995 which provides opportunity for public comment on new or revised data collections, the...

  6. 77 FR 25196 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-27

    ... of Immigration Appeals (Form EOIR-27) ACTION: 30-Day Notice of Information Collection Under Review.... Overview of This Information Collection: (1) Type of Information Collection: Revision of a currently... Representative Before the Board of Immigration Appeals. (3) Agency form number, if any, and the applicable...

  7. 78 FR 26087 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-03

    ...; Comment Request; Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery... for the Collection of Qualitative Feedback on Agency Service Delivery '' to OMB for approval under the.... SUPPLEMENTARY INFORMATION: Title: Generic Clearance for the Collection of Qualitative Feedback on Agency Service...

  8. 76 FR 10626 - Agency Information Collection Activities: Proposed Collection; Comment Request; Generic Clearance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-25

    ... Request; Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery AGENCY... Clearance for the Collection of Qualitative Feedback on Agency Service Delivery'' to OMB for approval under...: Title: Generic Clearance for the Collection of Qualitative Feedback on Agency Service Delivery. Abstract...

  9. 76 FR 28038 - Agency Information Collection Activities; Proposed Collection; Comment Request; General Services...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-13

    ... Administration Acquisition Regulation; Generic Clearance for the Collection of Qualitative Feedback on Agency... Clearance for the Collection of Qualitative Feedback on Agency Service Delivery'' to OMB for approval under... Collection of Qualitative Feedback on Agency Service Delivery, by any of the following methods: Regulations...

  10. 78 FR 9694 - Agency Information Collection Activities; Proposed Collection; Comment Request; MyGov

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-11

    ...] Agency Information Collection Activities; Proposed Collection; Comment Request; MyGov AGENCY: Office of... by Information Collection 3090- 00XX; MyGov by any of the following methods: Regulations.gov : http://www.regulations.gov . Submit comments via the Federal eRulemaking portal by searching for...

  11. 78 FR 69934 - Agency Information Collection Activities: Information Collection Renewal; Submission for OMB...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-21

    ... Activities: Information Collection Renewal; Submission for OMB Review; Lending Limits AGENCY: Office of the... concerning renewal of its information collection titled, ``Lending Limits.'' The OCC is also giving notice... INFORMATION: The OCC is seeking to renew, without change, the following collection: Title: Lending Limits--12...

  12. 77 FR 53890 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-04

    ... currently approved collection; Title of Information Collection: The Fiscal Soundness Reporting Requirements... those programs maintain fiscally sound organizations. The revised fiscal soundness reporting form...); Frequency: Annually, Quarterly; Affected Public: Private Sector: Business or other for-profits and Not-for...

  13. 77 FR 5279 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-02

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2012-0009] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The Nuclear Regulatory Commission (NRC) invites public comment about...

  14. 78 FR 45569 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-29

    ... NATIONAL ARCHIVES AND RECORDS ADMINISTRATION [NARA-2013-039] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: National Archives and Records Administration (NARA... make inquiries on their behalf and to release information and records related to their Freedom of...

  15. 76 FR 14433 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-16

    ... concerning the following information collection: 1. Title: Presidential Library Facilities. OMB number: 3095... Presidential library facility. The report contains information that can be furnished only by the foundation or... NATIONAL ARCHIVES AND RECORDS ADMINISTRATION Agency Information Collection Activities: Proposed...

  16. 76 FR 10905 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-28

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research and Quality (AHRQ), Department of Health and Human Services (HHS). ACTION: Notice. SUMMARY: The...

  17. Collection Development Plan.

    ERIC Educational Resources Information Center

    Minnesota State Law Library, St. Paul.

    This collection development plan of the Minnesota State Law Library includes detailed information on policies and annotations. After an overview of the Library's collection, general policy guidelines on the following are discussed: material selection; principles of selection; exclusions; gifts; interlibrary loan; cooperation; replacements;…

  18. 75 FR 44816 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-29

    ... NUCLEAR REGULATORY COMMISSION [NRC-2010-0263] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: U.S. Nuclear Regulatory Commission (NRC). ACTION: Notice of pending NRC... individual monitoring for occupational radiation exposure during a monitoring (one calendar year) period to...

  19. 77 FR 70192 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-23

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2012-0263] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  20. 76 FR 72982 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-28

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2011-0271] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  1. 75 FR 4848 - Agency Information Collection Activities: Proposed Collection, Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-29

    ... Collection Under Review: Revision of a currently approved collection: Law Enforcement Officers Killed or Assaulted. The Department of Justice, Federal Bureau of Investigation, Criminal Justice Information Services... DEPARTMENT OF JUSTICE Federal Bureau of Investigation [OMB Number 1110-0006] Agency Information...

  2. 75 FR 52027 - Agency Information Collection Activities: Proposed Collection, Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-24

    ... DEPARTMENT OF JUSTICE Federal Bureau of Investigation [OMB Number 1110-0015] Agency Information... collection under review: Extension of a currently approved collection, Hate Crime Incident Report; Quarterly Hate Crime Report. The Department of Justice, Federal Bureau of Investigation, Criminal Justice...

  3. 76 FR 24032 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-29

    ... phases: First, for planning; second, for early information technology development; and third, for... collected; and (4) the use of automated collection techniques or other forms of information technology to... the Cooperative Agreements to Support Innovative Exchange Information Technology Systems. Level One...

  4. 78 FR 21956 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-12

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Medicare & Medicaid Services [Document... currently approved collection; Title of Information Collection: Medicare Participating Physician or Supplier Agreement. Use: Section 1842(h) of the Social Security Act permits physicians and suppliers to voluntarily...

  5. 75 FR 39054 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-07

    ... NUCLEAR REGULATORY COMMISSION [NRC-2010-0234] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: U.S. Nuclear Regulatory Commission (NRC). ACTION: Notice of pending NRC..., or wishing to participate in a license application review for the potential geologic repository. 5...

  6. 77 FR 69661 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-20

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2012-0230] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  7. 78 FR 70353 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-25

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2013-0248] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  8. 77 FR 21813 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-11

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2012-0058] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  9. 77 FR 58872 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-24

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2012-0198] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  10. 78 FR 66078 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-04

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2013-0238] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  11. 78 FR 42112 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-15

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2013-0116] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  12. 77 FR 45697 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-01

    ... NUCLEAR REGULATORY COMMISSION [NRC-2012-0149] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending U.S. Nuclear... Budget and solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites...

  13. 77 FR 63893 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-17

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2012-0228] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC or the Commission) invites...

  14. 78 FR 28244 - Agency Information Collection Activities; Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-14

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2013-0085] Agency Information Collection Activities; Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  15. 75 FR 17438 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-06

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2010-0141] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: U.S. Nuclear Regulatory Commission (NRC). ACTION: Notice of... new Agreement State applications. Annually for participation by Agreement States in the IMPEP reviews...

  16. 77 FR 44289 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-27

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2012-0150] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  17. 76 FR 72983 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-28

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2011-0250] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  18. 75 FR 9444 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-02

    ... applications for licenses and amendments thereto to construct and operate nuclear power plants, preliminary or... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2010-0063] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: U.S. Nuclear Regulatory Commission (NRC). ACTION: Notice of...

  19. 77 FR 54617 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-05

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2012-0184] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  20. 77 FR 29697 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-18

    ... NUCLEAR REGULATORY COMMISSION [NRC-2012-0066] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending U.S. Nuclear... Budget and solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites...

  1. 76 FR 33788 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2011-0009] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... (OMB) and solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites...

  2. 76 FR 23628 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-27

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2011-0056] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission (NRC). ACTION: Notice of pending...: Applications for licenses are submitted once. Other reports are submitted annually or as other events require...

  3. 76 FR 1640 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-11

    ... of Information Collection Under Review: Prevent All Cigarette Trafficking (PACT) Act Registration... Cigarette Trafficking (PACT) Act Registration Form. (3) Agency form number, if any, and the applicable... this information collection is to register delivery sellers of cigarettes and/or smokeless tobacco...

  4. 78 FR 10620 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-14

    ... collection; Title of Information Collection: Organ Procurement Organization's (OPOs) Health Insurance Benefits Agreement and Supporting Regulations at 42 CFR 486.301-486.348; Use: The Medicare and Medicaid... DEPARTMENT OF HEALTH AND HUMAN SERVICES Centers for Medicare & Medicaid Services [Document...

  5. 78 FR 79500 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-30

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2013-0279] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  6. 76 FR 38655 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-01

    ... Request: New collection; Title of Information Collection: Analysis of Transportation Barriers to... of the proposed study is to identify and analyze transportation barriers associated with the... analyze the health outcomes resulting from those barriers, and ultimately to identify potential solutions...

  7. 76 FR 2390 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-13

    ... burden hours for the respondents' time to participate in this research. Among the 776 adolescent patients... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research...

  8. 77 FR 44290 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-27

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2012-0155] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  9. 77 FR 48555 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-14

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2012-0172] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC... solicitation of public comment. SUMMARY: The U.S. Nuclear Regulatory Commission (NRC) invites public comment...

  10. 78 FR 54261 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-03

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Substance Abuse and Mental Health Services Administration Agency Information Collection Activities: Proposed Collection; Comment Request In compliance with Section... include: The level to which the event met expectations; memory for information learned during the training...

  11. 77 FR 24223 - Agency Information Collection Activities: Proposed Collection; Comments Requested; Report of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-23

    ...] Agency Information Collection Activities: Proposed Collection; Comments Requested; Report of Theft or... of Theft or Loss of Explosives. (3) Agency form number, if any, and the applicable component of the... abstract: Primary: Business or other for-profit. Other: None. Need for Collection Losses or theft of...

  12. 76 FR 61739 - Agency Information Collection Activities; Comment Request for Information Collection; Information...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-05

    ... Collection; Information Collection Plan for Benefits.gov Online; Extension Without Change AGENCY: Office of...-free number) or e-mail to [email protected]gov . Send comments regarding this proposed collection of... Benefits.gov Web site. The Benefits.gov Web site assists citizens by providing information and eligibility...

  13. 76 FR 30741 - Agency Information Collection Activities: Existing Collection; Comments Requested: Prison...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-26

    ... Sentenced Population Movement--National Prisoner Statistics, Extension and Revision of Existing Collection...) Title of the Form/Collection: Summary of Sentenced Population Movement--National Prisoner Statistics (3...

  14. 75 FR 12227 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-15

    ... proposes to collect the following data from each MECS establishment: (1) For each energy source consumed--consumption (total, fuel and nonfuel uses) and the expenditures for each energy source, energy storage (as... DEPARTMENT OF ENERGY Energy Information Administration Agency Information Collection Activities...

  15. 78 FR 13687 - Agency Information Collection Activities; Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... verifying information, processing and maintaining information, and disclosing and providing information, to... Information Collection Activities; Proposed Collection; Comment Request ACTION: Notice. SUMMARY: In compliance... Paperwork Reduction Act of 1995. To request more information on the proposed project or to obtain a copy of...

  16. 75 FR 16132 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-31

    ... spectrum of antibacterial activity) and cannot be treated by the most commonly prescribed antibiotics... Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... support research on healthcare and on systems for the delivery of such care, including activities with...

  17. 75 FR 31445 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-03

    ... broad spectrum of antibacterial activity) and cannot be treated by the most commonly prescribed... Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... support research on healthcare and on systems for the delivery of such care, including activities with...

  18. 75 FR 67366 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-02

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  19. 75 FR 53305 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-31

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  20. 78 FR 41062 - Agency Information Collection Activities: Existing Collection; Emergency Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-09

    ... Information Collection--Emergency Extension without Change: Demographic Information on Applicants for Federal... gathering data on the race, sex and national origin status of applicants. Overview of Information Collection..., national origin, sex and disability status of applicants for both permanent and temporary employment...

  1. 76 FR 52698 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-23

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2011-0181] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission (NRC). ACTION: Notice of pending... administration of the byproduct material or the radiation there from to human beings or animals. Possession of...

  2. 75 FR 63866 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-18

    ... special nuclear material. The information in the applications, reports, and records is used by NRC to make... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2010-0322] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission (NRC). ACTION: Notice of pending...

  3. 75 FR 45676 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-03

    ... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2010-0235] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: U.S. Nuclear Regulatory Commission (NRC). ACTION: Notice of...: Applications for new licenses and amendments may be submitted at any time. Applications for renewal are...

  4. 78 FR 67204 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-08

    ... special nuclear material. The information in the applications, reports, and records is used by NRC to make... NUCLEAR REGULATORY COMMISSION [Docket No. NRC-2013-0239] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Nuclear Regulatory Commission. ACTION: Notice of pending NRC...

  5. 76 FR 55706 - Agency Information Collection Activities; Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-08

    ... of information collection: Certification on Agency Letterhead Authorizing Purchase of Firearm for... following four points: --Evaluate whether the proposed collection of information is necessary for the proper... Letterhead Authorizing Purchase of Firearm for Official Duties of Law Enforcement Officer. (3) Agency form...

  6. 76 FR 39416 - Agency Information Collection Activities; New Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-06

    ..., mechanical, or other technological collection techniques or other forms of information technology, e.g... of Qualitative Feedback on Agency Service Delivery. (3) Agency form number, if any, and the... governments; The information collection activity will garner qualitative customer and stakeholder feedback in...

  7. 77 FR 67656 - Agency Information Collection Activities: Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-13

    ... of information technology to minimize the information collection burden. Information Collection... to improve access to primary care, which is supported by clinicians who remain in their sites well... of learning how to bolster retention. Survey data will be gathered anonymously and presented in...

  8. 78 FR 73541 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-06

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  9. 76 FR 2121 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-12

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  10. 76 FR 17864 - Agency Information Collection Activities; Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-31

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities; Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  11. 78 FR 11645 - Agency Information Collection Activities: Final Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-19

    ... EXPORT-IMPORT BANK OF THE UNITED STATES [Public Notice 2013-0115] Agency Information Collection Activities: Final Collection; Comment Request AGENCY: Export-Import Bank of the United States. ACTION...-Im Bank approved insurance claims. Affected Public: This form affects entities involved in the export...

  12. 77 FR 58989 - Agency Information Collection Activities: Final Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-25

    ... EXPORT-IMPORT BANK [Public Notice 2012-0505] Agency Information Collection Activities: Final Collection; Comment Request AGENCY: Export-Import Bank of the U.S. ACTION: Submission for OMB Review and... Bank under its insurance, guarantee, and direct loan programs. Affected Public: This form affects...

  13. 77 FR 10746 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-23

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  14. 77 FR 14373 - Agency Information Collection Activities; Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-09

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities; Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  15. 75 FR 32783 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-09

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  16. 77 FR 40885 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-11

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  17. 76 FR 30172 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  18. 77 FR 24957 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-26

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  19. 76 FR 16783 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-25

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  20. 77 FR 30007 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-21

    ... smears and vaccines; poor self- management of conditions such as asthma and diabetes; and higher rates of... delivery of such care, including activities with respect to the quality, effectiveness, efficiency... Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research...

  1. 75 FR 75475 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-03

    ... collection; Title of Information Collection: Psychiatric Unit Criteria Work Sheet and Supporting Regulations... section 1886(d)(1)(B) of the Social Security Act. That section excludes psychiatric hospitals... (children's hospitals), and psychiatric and rehabilitation units which are a distinct part of a hospital...

  2. 78 FR 32652 - Agency Information Collections Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ... Reauthorization Act of 2009 (CHIPRA) Quality Demonstration Grant Program: Survey Data Collection.'' In accordance... Grant Program: Survey Data Collection. The Children's Health Insurance Program Reauthorization Act of... other States in designing and implementing their projects. Of the 10 grantee States selected, six are...

  3. 76 FR 71351 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-17

    ... with no or minimal interaction. Assessment and Referral Tool. This tool provides descriptive... crisis counselor. Participant Feedback. These surveys are completed by and collected from a sample of... Provider Feedback. These surveys are completed by and collected from the CCP service providers anonymously...

  4. 76 FR 27369 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-11

    ... information collection for three years. 1. Abstract The Survey of Graduate Students and Postdoctorates in... days a year (including Federal holidays). SUPPLEMENTARY INFORMATION: Title of Collection: Survey of Graduate Students and Postdoctorates in Science and Engineering. OMB Approval Number: 3145-0062. Expiration...

  5. 78 FR 45205 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-26

    ... associated materials (see ADDRESSES). CMS-10326 Electronic Submission of Medicare Graduate Medical Education... collection; Title of Information Collection: Electronic Submission of Medicare Graduate Medical Education... Education FTE cap slots are valid according to CMS regulations. The affiliation agreements are also used as...

  6. 78 FR 65318 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-31

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  7. 78 FR 46335 - Agency Information Collection Activities; Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-31

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities; Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  8. 78 FR 25085 - Agency Information Collection Activities; Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-29

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities; Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  9. 75 FR 9435 - Agency Information Collection Activities: Existing collection, Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-02

    ... collection for renewal. Three Fingerprint Cards: Arrest and Institution; Applicant; Personal Identification...-0046. (2) The title of the form/collection: Three Fingerprint Cards: Arrest and Institution; Applicant... Integrated Automated Fingerprint Identification System (IAFIS) of the FBI. (5) An estimate of the total...

  10. 76 FR 70726 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-15

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  11. 76 FR 46811 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  12. 75 FR 8995 - Agency Information Collection Activities: New Collection, Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-26

    ... Under Review: New collection; InfraGard Knowledge/Skills/Abilities Profile Questionnaire. The Department... submitting the following information collection requests to the Office of Management and Budget (OMB) for... contained in this notice, especially the estimated public burden and associated response time, should be...

  13. 76 FR 18582 - Agency Information Collection Activities: Proposed Collection: Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-04

    ... (OJP), Bureau of Justice Statistics (BJS), will be submitting the following information collection... Christine Eith, Bureau of Justice Statistics, 810 Seventh Street, NW., Washington, DC 20531 (phone: 202- 305... applicable component of the Department sponsoring the collection: PPCS-1. Bureau of Justice Statistics...

  14. 78 FR 23566 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-19

    ... utility of the proposed information collection for the proper performance of the agency's functions; (2... Information Collection: Consolidated Renal Operations in a Web Enabled Network (CROWNWeb) Third-party Submission Authorization Form Use: The Consolidated Renal Operations in a Web Enabled Network (CROWNWeb...

  15. 78 FR 946 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-07

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Agency for Healthcare Research and Quality Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research... Healthcare Research and Quality (AHRQ) to request that the Office of Management and Budget (OMB) approve the...

  16. 76 FR 52015 - Agency Information Collection Activities; Proposed Collection, Comments Requested; Establishment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-19

    ... Fingerprint Examiners Questionnaire ACTION: 30-Day Notice of Information Collection Under Review. The... title of the form/collection: National Fingerprint Examiners Questionnaire. (3) The agency form number...

  17. Collection Assessment. SPEC Kit 41.

    ERIC Educational Resources Information Center

    Association of Research Libraries, Washington, DC. Office of Management Studies.

    This Association of Research Libraries (ARL) kit on collection assessment contains the following documents: (1) "Guidelines for the Evaluation of Library Collections--Draft Copy" (Collection Development Committee, Resource Section, Resources and Technical Services Division, American Library Association); (2) "Guidelines for Collection Assessment"…

  18. 78 FR 1917 - Agency Information Collection Activities: Request for Comments for a New Information Collection

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-09

    ... support transportation research, capacity building, data collection, planning, travel modeling, and... Information Collection Activities: Request for Comments for a New Information Collection AGENCY: Federal... information collection, which is summarized below under SUPPLEMENTARY INFORMATION. We published a Federal...

  19. 75 FR 1082 - Civil Rights Division; Agency Information Collection Activities: Proposed Collection; Comments...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-08

    ... DEPARTMENT OF JUSTICE [OMB Number 1190-0001] Civil Rights Division; Agency Information Collection Activities: Proposed Collection; Comments Requested ACTION: 60[dash]Day Notice of Information Collection Under Review: Procedures for the Administration of Section 5 of the Voting Rights Act of 1965. The Department of Justice (DOJ), CRT will be...

  20. 76 FR 80403 - Agency Information Collection Activities; Proposed collection; Comments Request: Alien's Change...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-23

    ... collection; Comments Request: Alien's Change of Address Form: 33/BIA Board of Immigration Appeal; 33/IC... Collection. (2) Title of the Form/Collection: Alien's Change of Address Forms 33/BIA Board of Immigration... send notices of the next administrative action or of any decisions in an alien's case. (5) An estimate...

  1. 76 FR 28057 - Agency Information Collection Activities: New Information Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-13

    ... retrieves biometric and biographic data from the Automated Biometric Identification System (IDENT) within... information collection is necessary for USCIS to collect and process the required biometric and biographic...

  2. 75 FR 54215 - Agency Request for Approval of a Previously Approved Information Collection(s): Uniform...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-03

    ... Previously Approved Information Collection(s): Uniform Administrative Requirements for Grants and Cooperative... Higher Education, Hospitals, and Other Nonprofit Organizations AGENCY: Office of the Secretary. ACTION... approved information collection. This information collection involves the use of various forms necessary...

  3. 77 FR 6856 - Agency Requests Approval To Extend Information Collection(s): Section 410 Alcohol Impaired...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-09

    ... DEPARTMENT OF TRANSPORTATION National Highway Traffic Safety Administration [U.S. DOT Docket No. NHTSA-2012-0012] Agency Requests Approval To Extend Information Collection(s): Section 410 Alcohol... extend information collection. The collection involves preparation of certifications, and documents...

  4. Student Loan Collection Procedures.

    ERIC Educational Resources Information Center

    National Association of College and University Business Officers, Washington, DC.

    This manual on the collection of student loans is intended for the use of business officers and loan collection personnel of colleges and universities of all sizes. The introductory chapter is an overview of sound collection practices and procedures. It discusses the making of a loan, in-school servicing of the accounts, the exit interview, the…

  5. 77 FR 61001 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-05

    ... currently approved collection; Title of Information Collection: Bid Pricing Tool (BPT) for Medicare... organizations (MAO) and Prescription Drug Plans (PDP) are required to submit an actuarial pricing ``bid'' for... PDPs use the Bid Pricing Tool (BPT) software to develop their actuarial pricing bid. The information...

  6. 77 FR 284 - Agency Information Collection Activities: Final Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-04

    ... EXPORT-IMPORT BANK OF THE U.S. [Public Notice 2011-076] Agency Information Collection Activities: Final Collection; Comment Request AGENCY: Export-Import Bank of the U.S. ACTION: Submission for OMB... be considered in the overall evaluation of the transaction, including Export-Import Bank's...

  7. 75 FR 2547 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-15

    ... : 0938-0579); Frequency: Reporting--Triennially; Affected Public: Business or other for-profits and Not... change of a previously approved collection; Title of Information Collection: Business Proposal Forms for... contracts. The revised business proposal forms will be useful in a number of important ways. The Government...

  8. 76 FR 20044 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-11

    ... Form: State Criminal Alien Assistance Program. The Department of Justice (DOJ), Office of Justice... currently approved collection. (2) The title of the form/collection: State Criminal Alien Assistance Program... Section 130002(b) as amended in 1996, BJA administers the State Criminal Alien Assistance Program (SCAAP...

  9. 75 FR 39585 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-09

    ... NUCLEAR REGULATORY COMMISSION [NRC-2010-0236] Agency Information Collection Activities: Proposed Collection; Comment Request AGENCY: U.S. Nuclear Regulatory Commission (NRC). ACTION: Notice of pending NRC... licensees to ascertain compliance with the provisions of the Atomic Energy Act of 1954, as amended, Title II...

  10. 78 FR 15016 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-08

    ... permitted to use paid feeding assistants to supplement the services of certified nurse aides. If facilities choose this option, feeding assistants must complete a training program. Nursing home providers are... collection; Title of Information Collection: Paid Feeding Assistants in Long-Term Care Facilities and...

  11. 76 FR 71601 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-18

    ... technological collection techniques or other forms of information technology, e.g., permitting electronic...) Title of the Form/Collection: Report of Multiple Sale or Other Disposition of Pistols and Revolvers. (3... information documents certain sales or other dispositions of handguns for law enforcement purposes and...

  12. 75 FR 62401 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-08

    ... collection; Title of Information Collection: Clinical Laboratory Improvement Amendment (CLIA) of 1988 and... laboratories that perform testing on human beings to meet performance requirements (quality standards) in order... functions; (2) the accuracy of the estimated burden; (3) ways to enhance the quality, utility, and clarity...

  13. 77 FR 19667 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-02

    ... support research on health care and on systems for the delivery of such care, including activities with... Information Collection Activities: Proposed Collection; Comment Request AGENCY: Agency for Healthcare Research and Quality, HHS. ACTION: Notice. SUMMARY: This notice announces the intention of the Agency for...

  14. 75 FR 8722 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-25

    ... Efficient Health Care in Federal Government Administered or Sponsored Health Care Programs,'' performance... collection for the proper performance of the agency's functions; (2) the accuracy of the estimated burden; (3... of Information Collection: Part C and D Complaints Resolution Performance Measures: Use: Part C...

  15. 77 FR 46097 - Agency Information Collection Activities: Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-02

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Health Resources and Services Administration Agency Information Collection Activities: Proposed Collection: Comment Request In compliance with the requirement for... States Code, as amended by the Paperwork Reduction Act of 1995, Pub. L. 104-13), the Health Resources and...

  16. 78 FR 75925 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-13

    ... or Submission'' or ``More Search Options'' to find the information collection document(s) that are... found in each collection's supporting statement and associated materials (see ADDRESSES). [[Page 75926... treatment purposes. This information is vital to the certification process. Form Number: CMS-116 (OCN : 0938...

  17. 76 FR 10392 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    ... collection under review; Registrants' Inventory of Drugs Surrendered--DEA Form 41. The Department of Justice (DOJ), Drug Enforcement Administration (DEA), will be submitting the following information collection...--DEA Form 41. (3) Agency form number, if any, and the applicable component of the Department sponsoring...

  18. 75 FR 6708 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-10

    ... Explosives (ATF), will be submitting the following information collection request to the Office of Management... or associated response time, suggestions, or need a copy of the proposed information collection... Management Division, 99 New York Ave., NE.,Washington, DC 20226. Written comments and suggestions from the...

  19. 76 FR 17423 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-29

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Health Resources and Services Administration Agency Information Collection Activities: Proposed Collection; Comment Request In compliance with the requirements of Section 3506(c)(2)(A) of Public Law 104-13, the Paperwork Reduction Act of 1995, the Health Resources and...

  20. 76 FR 2697 - Agency Information Collection Activities: Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-14

    ... DEPARTMENT OF HEALTH AND HUMAN SERVICES Health Resources and Services Administration Agency Information Collection Activities: Proposed Collection: Comment Request In compliance with the requirement for... States Code, as amended by the Paperwork Reduction Act of 1995, Pub. L. 104-13), the Health Resources and...

  1. 78 FR 3008 - Agency Information Collection Activities: Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-15

    ... technology to minimize the information collection burden. Information Collection Request Title: Organ Donation/Transplant Life Stories (OMB No. 0915-xxxx)--[New] Abstract HRSA's Division of Transplantation (Do... visited pages on organdonor.gov are the donor and recipient life stories, which in a recent evaluation...

  2. 75 FR 60771 - Agency Information Collection Activities: New Information Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-01

    ... responses. Overview of This Information Collection (1) Type of Information Collection: New information... of time estimated for an average respondent to respond: ID Authentication--2,900,000 responses at...

  3. 76 FR 64377 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Alien's Change...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-18

    ... Collection; Comments Requested: Alien's Change of Address Forms: 33/BIA Board of Immigration Appeals and 33... approved collection. (2) Title of the Form/Collection: Alien's Change of Address Forms: 33/BIA Board of... determine where to send notices of the next administrative action or of any decisions in an alien's case. (5...

  4. 78 FR 41795 - Notice of Public Information Collection(s) Being Reviewed by the Federal Communications...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-11

    ... FEDERAL COMMUNICATIONS COMMISSION Notice of Public Information Collection(s) Being Reviewed by the... collection techniques or other forms of information technology; and further ways to reduce the information... existing information collection requirements would stay as they are. Federal Communications Commission...

  5. 76 FR 68787 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Extension of a...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-07

    ... Death ACTION: 60-Day notice of information collection under review. The Department of Justice (DOJ... collection. (2) Title of the Form/Collection: Claim for Damage, Injury, or Death. (3) Agency form number, if...

  6. 77 FR 35383 - Agency Information Collection Activities: Final Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-13

    ... EXPORT-IMPORT BANK OF THE U.S. [Public Notice 2012-0110] Agency Information Collection Activities: Final Collection; Comment Request AGENCY: Export-Import Bank of the U.S. ACTION: Submission for OMB Review and Comments Request. Form Title: EIB 11-05 Exporter's Certificate For Loan Guarantee & MT...

  7. 76 FR 39907 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-07

    ... NRC action to submit an information collection request to the Office of Management and Budget (OMB) and solicitation of public comment. SUMMARY: The NRC invites public comment about our intention to.... The title of the information collection: 10 CFR Part 74-- Material Control and Accounting of Special...

  8. 78 FR 14805 - Agency Information Collection Activities; Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-07

    ... technology to minimize the information collection burden. Information Collection Request Title: The Stem Cell Therapeutic Outcomes Database (OMB No. 0915-0310)--[Revision] Abstract: The Stem Cell Therapeutic and Research Act of 2005, Public Law (Pub. L.) 109-129, as amended by the Stem Cell Therapeutic and Research...

  9. 75 FR 52977 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-30

    ... --Minimize the burden of the collection of information on those who are to respond, including through the use... other forms of information technology, e.g., permitting electronic submission of responses. Overview of... firearm or firearms, ammunition, and the implements of war, and to facilitate the collection of...

  10. 76 FR 34076 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-10

    ... in use without an OMB control number; Title of Information Collection: Medicare Beneficiary and... Satisfaction flows from the proposed sampling approach. While it was feasible to conduct the 9th SOW via... not seem efficient to maintain a telephone only data collection approach. Based on recent literature...

  11. 75 FR 36443 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-25

    ... Emergency Notice of Information Collection Under Review: Prevent All Cigarette Trafficking (PACT) Act...) The title of the form/collection: Prevent All Cigarette Trafficking (PACT) Act Registration Form. (3... cigarettes and/or smokeless tobacco products with the Attorney General in order to continue to sell and/or...

  12. 75 FR 43526 - Agency Information Collection Activities; Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-26

    ... information collection requirements described below will be submitted to the Office of Management and Budget... collection requirements contained in its Affiliate Marketing Rule (or ``Rule''). That clearance expires on...MarketingPRA ) (and following the instructions on the web-based form). Comments filed in paper form should...

  13. 75 FR 41860 - Agency Information Collection Activities;Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... information collection requirements described below will be submitted to the Office of Management and Budget... collection requirements contained in its Affiliate Marketing Rule (or ``Rule''). That clearance expires on...MarketingPRA ) (and following the instructions on the web-based form). Comments filed in paper form should...

  14. 78 FR 954 - Agency Information Collection Activities: Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-07

    ... technology to minimize the information collection burden. Information Collection Request Title: Analyzing... as guide transformation in the delivery of medicine and public health services. For children... provisions of the ACA in 2014 will result in some shifts in child health coverage. The Title V Maternal and...

  15. 77 FR 65391 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-26

    ...) ways to enhance the quality, utility, and clarity of the information to be collected; and (4) the use of automated collection techniques or other forms of information technology to minimize the..., there is a focused interest in providing quality and value-based healthcare for Medicare beneficiaries...

  16. 77 FR 35679 - Agency Information Collection Activities: Final Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-14

    ... EXPORT-IMPORT BANK OF THE UNITED STATES [Public Notice 2012-0136] Agency Information Collection Activities: Final Collection; Comment Request AGENCY: Export-Import Bank of the U.S. ACTION: Submission for... Financial Institution of Medium Term Export Credit Insurance Policy. SUMMARY: The Export-Import Bank of the...

  17. 78 FR 22888 - Agency Information Collection Activities; Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-17

    ... respondents when there are missing data elements prior to submission. We acknowledge a change in the burden... with the requirement for opportunity for public comment on proposed data collection projects (Section... the data collection plans and draft instruments, email [email protected] or call the HRSA Reports...

  18. 76 FR 35445 - Agency Information Collection Activities: Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-17

    ... Information Collection: Medical Loss Ratio Quarterly Reporting; Use: Under Section 2718 of the Affordable Care... ratio, referred to as the medical loss ratio (MLR). An interim final rule (IFR) implementing the MLR was... information collections, please reference the document identifier or OMB control number. To be assured...

  19. 76 FR 3628 - Agency Information Collection Activities: Existing Collection; Emergency Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-20

    ... Collection; Emergency Extension AGENCY: Equal Employment Opportunity Commission. ACTION: Notice of Information Collection--Emergency Extension Without Change: Local Union Report (EEO-3). SUMMARY: In accordance... Division, 131 M Street, NE., Room 4SW30F, Washington, DC 20507; (202) 663-4958 (voice) or (202) 663-7063...

  20. 76 FR 38699 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-01

    ... Forfeiture and Money Laundering Section (AFMLS) will be submitting the following information collection... of Justice sponsoring the collection: Form Number: N/A. Criminal Division, Asset Forfeiture and Money Laundering Section. (4) Affected public who will be asked or required to respond, as well as a brief abstract...