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Sample records for hotel dearborn mi

  1. Dearborn: Modern Standard Arabic Instruction

    ERIC Educational Resources Information Center

    Education Week, 2006

    2006-01-01

    This article presents a roundup of the Arabic language instruction offered in the Dearborn, Michigan, school district. Only one of the district's 22 elementary schools--Becker--offers Arabic. Pupils receive at least two 40 minute periods of Arabic a week. The school gave up a two-way immersion program, in which students were taught half their…

  2. Chicago's Dearborn Observatory: a study in survival

    NASA Astrophysics Data System (ADS)

    Bartky, Ian R.

    2000-12-01

    The Dearborn Observatory, located on the Old University of Chicago campus from 1863 until 1888, was America's most promising astronomical facility when it was founded. Established by the Chicago Astronomical Society and directed by one of the country's most gifted astronomers, it boasted the largest telescope in the world and virtually unlimited operating funds. The Great Chicago Fire of 1871 destroyed its funding and demolished its research programme. Only via the sale of time signals and the heroic efforts of two amateur astronomers did the Dearborn Observatory survive.

  3. Source Apportionment of Ambient Fine Particulate Matter in Dearborn, Michigan, using Hourly Resolved PM Chemical Composition Data

    EPA Science Inventory

    High time-resolution aerosol sampling was conducted for one month during July–August 2007 in Dearborn, MI, a non-attainment area for fine particulate matter (PM2.5) National Ambient Air Quality Standards (NAAQS). Measurements of more than 30 PM2.5 species were made using a suite o...

  4. 77 FR 37326 - Safety Zone; Grand Hotel 125th Anniversary Fireworks Celebration, Mackinaw Island, MI

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-21

    .... SUPPLEMENTARY INFORMATION: Table of Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Grand Hotel 125th Anniversary Fireworks... launched from a point on Lake Huron to commemorate the Grand Hotel's 125th anniversary. The Captain of...

  5. Dearborn Forms Elementary Arabic Language Program Collaboration

    ERIC Educational Resources Information Center

    Tabrizi, Shereen

    2009-01-01

    The Dearborn Public Schools, with 18,300 students, is located in the Detroit urban area with the largest concentration of Arabic-speaking people in the United States. In order to prepare the students for the 21st century skills and global awareness and in response to parents' requests, the author in collaboration with the school principal and…

  6. 75 FR 3252 - Ford Motor Company, Dearborn Truck Plant, Dearborn, MI; Notice of Negative Determination on...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-20

    ... October 20, 2009 (74 FR 53766). The investigation resulted in a negative determination based on the... sports-utility pickups to/from a foreign country. The petitioners alleged that production at the subject... attached several newspaper articles, alleging that Ford manufactures pickups in Australia, South Africa...

  7. Sources of fine urban particulate matter in Detroit, MI.

    PubMed

    Gildemeister, Amy E; Hopke, Philip K; Kim, Eugene

    2007-10-01

    Data from the speciation trends network (STN) was used to evaluate the amount and temporal patterns of particulate matter originating from local industrial sources and long-range transport at two sites in Detroit, MI: Allen Park, MI, southwest of both Detroit and the areas of heavy industrial activity; Dearborn, MI, located on the south side of Detroit near the most heavily industrialized region. Using positive matrix factorization (PMF) and comparing source contributions at Allen Park to those in Dearborn, contributions made by local industrial sources (power plants, coke refineries, iron smelting, waste incineration), local area sources (automobile and diesel truck) and long range sources of PM(2.5) can be distinguished in greater Detroit. Overall, the mean mass concentration measured at Dearborn was 19% higher than that measured at Allen Park. The mass at Allen Park was apportioned as: secondary sulfate 31%, secondary nitrate 28%, soil 8%, mixed aged sea and road salts 4%, gasoline 15%, diesel 4%, and biomass burning 3%. At Dearborn the mass was apportioned as: secondary sulfate 25%, secondary nitrate 20%, soil 12%, mixed aged sea and road salts 4%, gasoline 20%, diesel 8%, iron and steel, 5%, and mixed industrial 7%. The impact of the iron and steel, soil, and mixed aged sea and road salt was much higher at the Dearborn site than at the Allen Park site, suggesting that close proximity to a local industrial complex has a direct negative impact on local air quality. PMID:17537480

  8. SITE TECHNOLOGY CAPSULE: GRACE DEARBORN INC.'S DARAMEND BIOREMEDIATION TECHNOLOGY

    EPA Science Inventory

    Grace Dearborn's DARAMEND Bioremediation Technology was developed to treat soils/sediment contaminated with organic contaminants using solid-phase organic amendments. The amendments increase the soil's ability to supply biologically available water/nutrients to microorganisms and...

  9. Industrial laser interferometry II; Proceedings of the Meeting, Dearborn, MI, June 27, 28, 1988

    SciTech Connect

    Hung, M.Y.Y.; Pryputniewicz, R.

    1988-01-01

    Various papers on industrial laser interferometry are presented. Individual topics discussed include: specklegrammetry for precision surface coordinate measurement, fast detection of residual stresses by shearography, surface inspection of automotive bodies by reflective computer vision, shearographic detection of flaws in unity vision optical components, coherent information process of white light speckle sandwich, coherent sensors for hostile environments, and grating method for strain measurement. Also considered are: Talbot carrier image processing of birefringence effect, advancement in photocarrier Talbot effect theory, shearing photoelasticity, study of brake squeal problem by pulsed holographic interferometry, modeling of drill bit transverse vibrations, use of glass fiber techniques in holographic deformation analysis, and vibration studies using heterodyne hologram interferometry.

  10. Source apportionment of ambient fine particulate matter in Dearborn, Michigan, using hourly resolved PM chemical composition data.

    PubMed

    Pancras, Joseph Patrick; Landis, Matthew S; Norris, Gary A; Vedantham, Ram; Dvonch, J Timothy

    2013-03-15

    High time-resolution aerosol sampling was conducted for one month during July-August 2007 in Dearborn, MI, a non-attainment area for fine particulate matter (PM2.5) National Ambient Air Quality Standards (NAAQS). Measurements of more than 30 PM2.5 species were made using a suite of semi-continuous sampling and monitoring instruments. Dynamic variations in the sub-hourly concentrations of source 'marker' elements were observed when discrete plumes from local sources impacted the sampling site. Hourly averaged PM2.5 composition data for 639 samples were used to identify and apportion PM2.5 emission sources using the multivariate receptor modeling techniques EPA Positive Matrix Factorization (PMF) v4.2 and EPA Unmix v6.0. Source contribution estimates from PMF and Unmix were then evaluated using the Sustained Wind Instance Method (SWIM), which identified plausible source origins. Ten sources were identified by both PMF and Unmix: (1) secondary sulfate, (2) secondary nitrate characterized by a significant diurnal trend, (3) iron and steel production, (4) a potassium-rich factor attributable to iron/steel slag waste processing, (5) a cadmium-rich factor attributable to incineration, (6) an oil refinery characterized by La/Ce>1 specific to south wind, (7) oil combustion, (8) coal combustion, (9) motor vehicles, and (10) road dust enriched with organic carbon. While both models apportioned secondary sulfate, oil refinery, and oil combustion PM2.5 masses closely, the mobile and industrial source apportionments differed. Analyses were also carried out to help infer time-of-day variations in the contributions of local sources. PMID:23302684

  11. 57. VIEW LOOKING NORTH OF DENNIS HOTEL. BLENHEIM HOTEL IS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    57. VIEW LOOKING NORTH OF DENNIS HOTEL. BLENHEIM HOTEL IS PARTLY VISABLE TO THE RIGHT; SHELBOURNE HOTEL IS PARTLY VISABLE TO THE LEFT - Marlborough, Blenheim & Dennis Hotels (aerial views), Between Park Place, Michigan Avenue & Boardwalk, Atlantic City, Atlantic County, NJ

  12. 54. VIEW LOOKING NORTHEAST AT DENNIS HOTEL, BLENHEIM HOTEL AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    54. VIEW LOOKING NORTHEAST AT DENNIS HOTEL, BLENHEIM HOTEL AND MARLBOROUGH HOTEL (LEFT TO RIGHT) - Marlborough, Blenheim & Dennis Hotels (aerial views), Between Park Place, Michigan Avenue & Boardwalk, Atlantic City, Atlantic County, NJ

  13. 66. VIEW LOOKING NORTH AT THE MARLBOROUGH HOTEL. BLENHEIM HOTEL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    66. VIEW LOOKING NORTH AT THE MARLBOROUGH HOTEL. BLENHEIM HOTEL IS PARTIALLY VIASBLE TO THE LEFT. CLARIDGE HOTEL IS VISABLE TO THE RIGHT - Marlborough, Blenheim & Dennis Hotels (aerial views), Between Park Place, Michigan Avenue & Boardwalk, Atlantic City, Atlantic County, NJ

  14. 41. VIEW LOOKING NORTHWEST AT THE DENNIS HOTEL, BLENHEIM HOTEL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    41. VIEW LOOKING NORTHWEST AT THE DENNIS HOTEL, BLENHEIM HOTEL AND MARLBOROUGH HOTEL (LEFT TO RIGHT). CLARIDGE HOTEL IS PARTLY VISABLE TO THE RIGHT - Marlborough, Blenheim & Dennis Hotels (aerial views), Between Park Place, Michigan Avenue & Boardwalk, Atlantic City, Atlantic County, NJ

  15. The Infinite Hotel

    ERIC Educational Resources Information Center

    Wanko, Jeffrey J.

    2009-01-01

    This article provides a historical context for the debate between Georg Cantor and Leopold Kronecker regarding the cardinality of different infinities and incorporates the short story "Welcome to the Hotel Infinity," which uses the analogy of a hotel with an infinite number of rooms to help explain this concept. Wanko makes use of this history and…

  16. 1. EAST FACADE OF THE MARCUS DALY HOTEL. THE HOTEL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. EAST FACADE OF THE MARCUS DALY HOTEL. THE HOTEL WAS CONSTRUCTED IN A U SHAPE, WITH THE ENTRANCE ARCADE FORMING THE FORTH SIDE OF THE RECTANGLE - Anaconda Historic District, Marcus Daly Hotel, 200-208 Main Street, Anaconda, Deer Lodge County, MT

  17. 52. VIEW LOOKING EAST WITH THE MARLBOROUGH HOTEL, BLENHEIM HOTEL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    52. VIEW LOOKING EAST WITH THE MARLBOROUGH HOTEL, BLENHEIM HOTEL AND THE DENNIS HI=OTEL (TOP TO BOTTOM) IN THE CENTER OF THE PHOTOGRAPH - Marlborough, Blenheim & Dennis Hotels (aerial views), Between Park Place, Michigan Avenue & Boardwalk, Atlantic City, Atlantic County, NJ

  18. Integrating Title I and Title VII: The Evolving Model of Dearborn Public Schools, Michigan. NCBE Program Information Guide Series No. 21.

    ERIC Educational Resources Information Center

    Arraf, Shereen; And Others

    This report offers insights on the experiences and techniques used in the Dearborn Public Schools effort to integrate Title VII and Chapter 1 programs prior to their reauthorization. The school system is located near metropolitan Detroit, Michigan, and has over 14,500 students representing 24 languages, many from the Middle East, Romania, and…

  19. Cogeneration for resort hotels

    SciTech Connect

    Baker, T.D.

    1986-01-01

    Resort Hotels should be considered for application of co-generation to take advantage of higher thermal efficiency and consequent energy cost avoidance. Modern resort hotels require comfort and reliability from mechanical and electrical systems on an around the clock basis. Load profiling reveals simultaneous process heating and electricity use requirements that aid in the selection and sizing of co-generation equipment. Resort Hotel needs include electrical loads for lighting, fan motors, elevators, escalators and receptacle uses. Process heat demands arise from kitchen, servery, banquet, restaurant, laundry, and bakery functions. Once the loads requiring service have been quantified and realigned (shifted) to maximize simultaneous demands the engineering task of co-generation application becomes one of economics. National legislation is now in place to foster the use of co-generating central utility plants. Serving utility companies are now by law required to buy back excess energy during periods of reduced hotel demands. Resort Hotel loads, converted into electricity and heat demands are tabulated in terms of savings (positive cash flow) or costs (negative cash flows). Cash flow tabulations expressed in graphs are included. The graphs show the approximate simple payback on initial costs of co-generation systems based on varying electricity charges.

  20. Quantum Hilbert Hotel

    NASA Astrophysics Data System (ADS)

    Potoček, Václav; Miatto, Filippo M.; Mirhosseini, Mohammad; Magaña-Loaiza, Omar S.; Liapis, Andreas C.; Oi, Daniel K. L.; Boyd, Robert W.; Jeffers, John

    2015-10-01

    In 1924 David Hilbert conceived a paradoxical tale involving a hotel with an infinite number of rooms to illustrate some aspects of the mathematical notion of "infinity." In continuous-variable quantum mechanics we routinely make use of infinite state spaces: here we show that such a theoretical apparatus can accommodate an analog of Hilbert's hotel paradox. We devise a protocol that, mimicking what happens to the guests of the hotel, maps the amplitudes of an infinite eigenbasis to twice their original quantum number in a coherent and deterministic manner, producing infinitely many unoccupied levels in the process. We demonstrate the feasibility of the protocol by experimentally realizing it on the orbital angular momentum of a paraxial field. This new non-Gaussian operation may be exploited, for example, for enhancing the sensitivity of NOON states, for increasing the capacity of a channel, or for multiplexing multiple channels into a single one.

  1. 27 CFR 31.82 - Hotels.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Hotels. 31.82 Section 31... Same Premises § 31.82 Hotels. The proprietor of a hotel who conducts the sale of liquors throughout the hotel premises is only required to register under this part for one place. For example, different...

  2. 27 CFR 31.82 - Hotels.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Hotels. 31.82 Section 31... Same Premises § 31.82 Hotels. The proprietor of a hotel who conducts the sale of liquors throughout the hotel premises is only required to register under this part for one place. For example, different...

  3. 27 CFR 31.82 - Hotels.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false Hotels. 31.82 Section 31... Same Premises § 31.82 Hotels. The proprietor of a hotel who conducts the sale of liquors throughout the hotel premises is only required to register under this part for one place. For example, different...

  4. 27 CFR 31.82 - Hotels.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false Hotels. 31.82 Section 31... Same Premises § 31.82 Hotels. The proprietor of a hotel who conducts the sale of liquors throughout the hotel premises is only required to register under this part for one place. For example, different...

  5. 27 CFR 31.82 - Hotels.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false Hotels. 31.82 Section 31... Same Premises § 31.82 Hotels. The proprietor of a hotel who conducts the sale of liquors throughout the hotel premises is only required to register under this part for one place. For example, different...

  6. Agency-Hired Hotel Housekeepers

    PubMed Central

    Sanon, Marie-Anne V.

    2014-01-01

    Hotel housekeepers experience unique workplace hazards and characteristics that increase their risks for poor health outcomes. Today’s agency-hiring practices may further marginalize hotel housekeepers and negatively impact their health. Yet the impact of such hiring practices on the health of this vulnerable worker group remains unexplored. This article presents the debate regarding agency-hiring practices and how these practices may influence the health and well-being of hotel housekeepers. Implications for occupational health nurses are also discussed. PMID:24512722

  7. Towards Universal Design Hotels in Denmark.

    PubMed

    Grangaard, Sidse

    2016-01-01

    Based on the research project 'Accessible Hotel Rooms' that studies the balance between the experience of supply and demand regarding accessibility features in Danish hotel rooms, this paper demonstrates factors having an influence on Universal Design hotels in Denmark. The research project was financed by the Danish Transport and Construction Agency. Different notions in the hotel sector of the current supply and demand for Universal Design hotel rooms are identified, as well as future demand. Despite supplying accessible rooms, some hotels do not advertise their accessibility features on their website. There exists an attitude in the hotel sector that functions as a barrier for Universal Design: if there are enough guests, for example business travellers, then why market the hotel on Universal Design? The paper points out the coherence between the understanding of the users and the view of demand. Another important factor is Corporate Social Responsibility, which can be regarded as a strategy or platform towards Universal Design hotels. PMID:27534313

  8. 27. VIEW LOOKING NORTH AT ROOFS OF DENNIS HOTEL AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. VIEW LOOKING NORTH AT ROOFS OF DENNIS HOTEL AND BLENHEIM HOTEL. MARLBOROUGH HOTEL IS PARTLY VISIBLE TO THE RIGHT - Marlborough, Blenheim & Dennis Hotels (aerial views), Between Park Place, Michigan Avenue & Boardwalk, Atlantic City, Atlantic County, NJ

  9. 6. VIEW LOOKING NORTH TO NORTHEAST OF DENNIS HOTEL, BLENHEIM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. VIEW LOOKING NORTH TO NORTHEAST OF DENNIS HOTEL, BLENHEIM HOTEL AND MARLBOROUGH HOTEL (LEFT TO RIGHT) IN THE CENTER OF THE PHOTO - Marlborough, Blenheim & Dennis Hotels (aerial views), Between Park Place, Michigan Avenue & Boardwalk, Atlantic City, Atlantic County, NJ

  10. Human Resource Development and New Technology in the Automobile Industry: A Case Study of Ford Motor Company's Dearborn Engine Plant. The Development and Utilization of Human Resources in the Context of Technological Change and Industrial Restructuring.

    ERIC Educational Resources Information Center

    Chen, Kan; And Others

    This report centers around a plant-level study of the development and utilization of human resources in the context of technological change and industrial restructuring in the crankshaft production area of Ford Motor Company's Dearborn Engine Plant (DEP). The introductory chapter describes how the study was conducted, provides an introduction to…

  11. Proceedings of the Eastern Michigan University Conference on Languages for Business and the Professions (4th, Dearborn, MI, May 2-4, 1985).

    ERIC Educational Resources Information Center

    Voght, Geoffrey M., Comp.

    The proceedings of this conference include 56 papers on program design, classroom techniques, and ideas for second language instruction for business and the professions. The papers are presented in eight groups: (1) "International Business: The Language and Culture Connection," which includes papers on multinational business, marketing,…

  12. Hotels Make Room for Fitness.

    ERIC Educational Resources Information Center

    Koszuta, Laurie Einstein

    1986-01-01

    Hotels, in hopes of gaining a competitive edge, are offering workout rooms, exercise equipment, fitness trails, and jogging tracks, but no standards have been set for safety of the facilities or staff preparedness in exercise screening, equipment use, injury prevention, or first aid. (MT)

  13. Room-Maid in Hotel

    ERIC Educational Resources Information Center

    Hotel and Catering Industry Training Board, Wembley (England).

    This syllabus is intended for the use of training personnel in drawing up training programs for room-maids in hotels. Its main objective is to produce fully trained maids, thereby maintaining and raising standards. The syllabus is divided into three sections: Introducing to Housekeeping, Basic Tasks Performed by the Majority of Housekeeping…

  14. 4. photocopy of an advertisement (from Penn Alto Hotel archives, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. photocopy of an advertisement (from Penn Alto Hotel archives, Altoona, Pennsylvania) ADVERTISEMENT TO SELL STOCK IN PENN ALTO HOTEL - Penn Alto Hotel, 1120-1130 Thirteenth Avenue, Altoona, Blair County, PA

  15. 33. VIEW LOOKING NORTHNORTHWEST AT THE DENNIS HOTEL (PARTIAL VIEW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    33. VIEW LOOKING NORTH-NORTHWEST AT THE DENNIS HOTEL (PARTIAL VIEW OF THE BLENHEIM HOTEL) - Marlborough, Blenheim & Dennis Hotels (aerial views), Between Park Place, Michigan Avenue & Boardwalk, Atlantic City, Atlantic County, NJ

  16. 43. VIEW LOOKING WEST AT THE MARLBOROUGH HOTEL WITH THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    43. VIEW LOOKING WEST AT THE MARLBOROUGH HOTEL WITH THE BLENHEIM, DENNIS,AND SHELBOURNE HOTELS BEYOUND - Marlborough, Blenheim & Dennis Hotels (aerial views), Between Park Place, Michigan Avenue & Boardwalk, Atlantic City, Atlantic County, NJ

  17. 39. VIEW LOOKING NORTHNORTHEAST AT THE MARLBOROUGH HOTEL WITH THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    39. VIEW LOOKING NORTH-NORTHEAST AT THE MARLBOROUGH HOTEL WITH THE BLENHEIM HOTEL IN THE BACKGROUND - Marlborough, Blenheim & Dennis Hotels (aerial views), Between Park Place, Michigan Avenue & Boardwalk, Atlantic City, Atlantic County, NJ

  18. CONTEXTUAL VIEW FROM HOTEL; HAMILTON BUNGALOW IN FOREGROUND; BUNGALOW NO. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CONTEXTUAL VIEW FROM HOTEL; HAMILTON BUNGALOW IN FOREGROUND; BUNGALOW NO. 3 DIRECTLY BEHIND; HINDS & CONNER AND "A" BUNGALOWS IN REAR. VISTA DEL ARROYO HOTEL ON RIGHT - Vista del Arroyo Hotel, 125 South Grand Avenue, Pasadena, Los Angeles County, CA

  19. 1. General view of the Moody Hotel, Tremont Square. The ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. General view of the Moody Hotel, Tremont Square. The hotel was built by William Emerson in 1890-92. - Claremont Village Industrial District, Moody Hotel, Tremont Square, Claremont, Sullivan County, NH

  20. 88. VIEW LOOKING FROM THE BRIDGE FROM THE MARLBOROUGH HOTEL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    88. VIEW LOOKING FROM THE BRIDGE FROM THE MARLBOROUGH HOTEL TOWARD THE READING LOUNGE OUTSIDE OF THE MAIN DINING ROOM OF THE BLENHEIM HOTEL - Blenheim Hotel, Ohio Avenue & Boardwalk, Atlantic City, Atlantic County, NJ

  1. 3. photocopy of an advertisement (from Penn Alto Hotel archives, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. photocopy of an advertisement (from Penn Alto Hotel archives, Altoona, Pennsylvania) ADVERTISEMENT TO SELL STOCK IN PENN ALTO HOTEL - Penn Alto Hotel, 1120-1130 Thirteenth Avenue, Altoona, Blair County, PA

  2. Hotel Housekeeping Work Influences on Hypertension Management

    PubMed Central

    Sanon, Marie-Anne

    2013-01-01

    Background Characteristics of hotel housekeeping work increase the risk for hypertension development. Little is known about the influences of such work on hypertension management. Methods For this qualitative study, 27 Haitian immigrant hotel housekeepers from Miami-Dade County, FL were interviewed. Interview transcripts were analyzed with the assistance of the Atlas. ti software for code and theme identification. Results Influences of hotel housekeeping work on hypertension management arose both at the individual and system levels. Factors at the individual level included co-worker dynamics and maintenance of transmigrant life. Factors at the system level included supervisory support, workload, work pace, and work hiring practices. No positive influences were reported for workload and hiring practices. Conclusions Workplace interventions may be beneficial for effective hypertension management among hotel housekeepers. These work influences must be considered when determining effective methods for hypertension management among hotel housekeepers. PMID:23775918

  3. 30. VIEW LOOKING NORTH AT DENNIS HOTEL SOUTH ELEVATION. BLENHEIM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. VIEW LOOKING NORTH AT DENNIS HOTEL SOUTH ELEVATION. BLENHEIM HOTEL SOUTH ELEVATION IS PARTIALLY VISIBLE TO THE RIGHT. SHELBOURNE HOTEL IS PARTIALLY VISIBLE TO THE LEFT - Marlborough, Blenheim & Dennis Hotels (aerial views), Between Park Place, Michigan Avenue & Boardwalk, Atlantic City, Atlantic County, NJ

  4. Geography Matters in Online Hotel Reviews

    NASA Astrophysics Data System (ADS)

    Wang, Mingshu; Zhou, Xiaolu

    2016-06-01

    In resonance with the popularity of user-generated contents (UGC) and the volunteered geographic information (VGI), this study crowdsourced 77,098 hotel reviews of 220 hotels provided by U.S. reviewers in the city of San Francisco, 2002 to 2015. In this exploratory analysis, we have revealed that there is spatial dependence of customer satisfaction at different locations (of hotels), which violates the assumption that ordinary least-square (OLS) is the best linear unbiased estimator (BLUE); therefore, spatial model might be required for analysing any antecedents and consequences of such phenomena. These results have implications in marketing and management strategies.

  5. Smoke management system considerations for hotel atriums

    SciTech Connect

    Dillon, M.E.

    1983-07-01

    This article examines the many life safety problems inherent in open balcony atriums, particularly those in hotels. Focuses on the smoke-management system of a hotel, and shows what can be achieved by an architect, an owner, a building department and a fire department that works together to achieve an enhanced level of life safety in the building's construction. Emphasizes that the hotel should be fully sprinklered; that it have carefully thought-out detection and alarm systems; that it have a reliably engineered smoke management system; and that it be routinely inspected and maintained. Discusses life safety concerns, smoke extraction, and failure mode analysis.

  6. Proceedings of the EMU Conference on Foreign Languages for Business and the Professions (Dearborn, Michigan, April 5-7, 1984). Part VI: French for Business and the Professions.

    ERIC Educational Resources Information Center

    Voght, Geoffrey M., Ed.

    Part VI of the proceedings includes 12 presentations. They are: "Teaching Students How to Read Economics and Commercial Texts in a Commercial French Course: Focus on Meaning" (Robert A. Kreiter); "The Teaching of French for Computers and Information Science" (John B. Romeiser); "A Program in French for Hotel and Restaurant Management Majors: The…

  7. Proceedings of the EMU Conference on Foreign Languages for Business and the Professions (Dearborn, Michigan, April 5-7, 1984). Part II: Program Overviews and Components.

    ERIC Educational Resources Information Center

    Voght, Geoffrey M., Ed.

    Part II of the proceedings includes nine presentations. They are: "Business and Foreign Language Tie the Knot at Nazareth College: A Four Year Program Model" (Octave G. Naulleau); "For an Actual Education in International Management" (Alain Eclache and Georges Labet); "A Foreign Language Program for Majors in Hotel and Restaurant Management:…

  8. The homeless: help from hotels and restaurants.

    PubMed

    Hales, A; Eyster, J J; Ford, J L

    1993-07-01

    Specific examples and information are given to service providers to address the needs of homeless people. Together nurses and restaurant and hotel managers combined their expertise to assist local agencies in their community kitchens and shelters. PMID:8321464

  9. Spinning reserve from hotel load response

    SciTech Connect

    Kirby, Brendan; Kueck, John; Laughner, Theo; Morris, Keith

    2008-12-15

    Even though preliminary tests were not conducted during times of highest system or hotel loading during the summer, they showed that hotel load can be curtailed by 22 to 37 percent depending on the outdoor temperature and time of day. Full response occurred in 12 to 60 seconds from when the system operator's command to shed load was issued and the load drop was very rapid. (author)

  10. Detail, Scandia Hotel, view to southwest showing details of balloon ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail, Scandia Hotel, view to southwest showing details of balloon framing, including full two-story studs notched to carry girts supporting second story floor joists (210mm lens) - Scandia Hotel, 225 First Street, Eureka, Humboldt County, CA

  11. Integration of spatio-temporal contrast sensitivity with a multi-slice channelized Hotelling observer

    NASA Astrophysics Data System (ADS)

    Avanaki, Ali N.; Espig, Kathryn S.; Marchessoux, Cedric; Krupinski, Elizabeth A.; Bakic, Predrag R.; Kimpe, Tom R. L.; Maidment, Andrew D. A.

    2013-03-01

    Barten's model of spatio-temporal contrast sensitivity function of human visual system is embedded in a multi-slice channelized Hotelling observer. This is done by 3D filtering of the stack of images with the spatio-temporal contrast sensitivity function and feeding the result (i.e., the perceived image stack) to the multi-slice channelized Hotelling observer. The proposed procedure of considering spatio-temporal contrast sensitivity function is generic in the sense that it can be used with observers other than multi-slice channelized Hotelling observer. Detection performance of the new observer in digital breast tomosynthesis is measured in a variety of browsing speeds, at two spatial sampling rates, using computer simulations. Our results show a peak in detection performance in mid browsing speeds. We compare our results to those of a human observer study reported earlier (I. Diaz et al. SPIE MI 2011). The effects of display luminance, contrast and spatial sampling rate, with and without considering foveal vision, are also studied. Reported simulations are conducted with real digital breast tomosynthesis image stacks, as well as stacks from an anthropomorphic software breast phantom (P. Bakic et al. Med Phys. 2011). Lesion cases are simulated by inserting single micro-calcifications or masses. Limitations of our methods and ways to improve them are discussed.

  12. 40 CFR 30.18 - Hotel and motel fire safety.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 1 2014-07-01 2014-07-01 false Hotel and motel fire safety. 30.18... EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Pre-Award Requirements § 30.18 Hotel and motel fire safety. The Hotel and Motel Fire Safety Act of 1990 (Public Law 101-391) establishes a number of...

  13. 40 CFR 30.18 - Hotel and motel fire safety.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Hotel and motel fire safety. 30.18... EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Pre-Award Requirements § 30.18 Hotel and motel fire safety. The Hotel and Motel Fire Safety Act of 1990 (Public Law 101-391) establishes a number of...

  14. 40 CFR 30.18 - Hotel and motel fire safety.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 1 2013-07-01 2013-07-01 false Hotel and motel fire safety. 30.18... EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Pre-Award Requirements § 30.18 Hotel and motel fire safety. The Hotel and Motel Fire Safety Act of 1990 (Public Law 101-391) establishes a number of...

  15. 40 CFR 30.18 - Hotel and motel fire safety.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Hotel and motel fire safety. 30.18... EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Pre-Award Requirements § 30.18 Hotel and motel fire safety. The Hotel and Motel Fire Safety Act of 1990 (Public Law 101-391) establishes a number of...

  16. 40 CFR 30.18 - Hotel and motel fire safety.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 1 2012-07-01 2012-07-01 false Hotel and motel fire safety. 30.18... EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Pre-Award Requirements § 30.18 Hotel and motel fire safety. The Hotel and Motel Fire Safety Act of 1990 (Public Law 101-391) establishes a number of...

  17. Hotel Employees' Japanese Language Experiences: Implications and Suggestions.

    ERIC Educational Resources Information Center

    Makita-Discekici, Yasuko

    1998-01-01

    Analyzes the Japanese language learning experiences of 13 hotel employees in Guam. Results of the study present implications and suggestions for a Japanese language program for the hotel industry. The project began as a result of hotel employees frustrations when they were unable to communicate effectively with their Japanese guests. (Auth/JL)

  18. Jebel Ali Hotel PV lighting systems

    SciTech Connect

    Ellis, M.

    1984-05-01

    A large stand-alone PV lighting project was installed in June 1983 at the Jebel Ali Hotel in Dubai, United Arab Emirates. A high mast lighting system provides illumination for a 130 meter diameter traffic roundabout. The high mast system is powered by a 15 kilowatt peak array of Mobil Solar ribbon PV modules. Along the 700 meter access road leading to the hotel entrance, twenty-one PV powered streetlights provide low-level lighting. Each streetlight consists of a 20 watt fluorescent tube powered by two 35 Wp modules. Operation of both systems is completely automatic. Design, installation, and operating experience to date are reviewed.

  19. Measurement of the direct <mi>CP> -violating parameter <mi>Ami><mi>CP> in the decay <mi>D>+<mi>Kmi>-<mimi>+<mi>π>+

    SciTech Connect

    Abazov, V. M.; Abbott, B.; Acharya, B. S.; Adams, M.; Adams, T.; Agnew, J. P.; Alexeev, G. D.; Alkhazov, G.; Alton, A.; Askew, A.; Atkins, S.; Augsten, K.; Avila, C.; Badaud, F.; Bagby, L.; Baldin, B.; Bandurin, D. V.; Banerjee, S.; Barberis, E.; Baringer, P.; Bartlett, J. F.; Bassler, U.; Bazterra, V.; Bean, A.; Begalli, M.; Bellantoni, L.; Beri, S. B.; Bernardi, G.; Bernhard, R.; Bertram, I.; Besançon, M.; Beuselinck, R.; Bhat, P. C.; Bhatia, S.; Bhatnagar, V.; Blazey, G.; Blessing, S.; Bloom, K.; Boehnlein, A.; Boline, D.; Boos, E. E.; Borissov, G.; Borysova, M.; Brandt, A.; Brandt, O.; Brock, R.; Bross, A.; Brown, D.; Bu, X. B.; Buehler, M.; Buescher, V.; Bunichev, V.; Burdin, S.; Buszello, C. P.; Camacho-Pérez, E.; Casey, B. C. K.; Castilla-Valdez, H.; Caughron, S.; Chakrabarti, S.; Chan, K. M.; Chandra, A.; Chapon, E.; Chen, G.; Cho, S. W.; Choi, S.; Choudhary, B.; Cihangir, S.; Claes, D.; Clutter, J.; Cooke, M.; Cooper, W. E.; Corcoran, M.; Couderc, F.; Cousinou, M. -C.; Cutts, D.; Das, A.; Davies, G.; de Jong, S. J.; De La Cruz-Burelo, E.; Déliot, F.; Demina, R.; Denisov, D.; Denisov, S. P.; Desai, S.; Deterre, C.; DeVaughan, K.; Diehl, H. T.; Diesburg, M.; Ding, P. F.; Dominguez, A.; Dubey, A.; Dudko, L. V.; Duperrin, A.; Dutt, S.; Eads, M.; Edmunds, D.; Ellison, J.; Elvira, V. D.; Enari, Y.; Evans, H.; Evdokimov, V. N.; Fauré, A.; Feng, L.; Ferbel, T.; Fiedler, F.; Filthaut, F.; Fisher, W.; Fisk, H. E.; Fortner, M.; Fox, H.; Fuess, S.; Garbincius, P. H.; Garcia-Bellido, A.; García-González, J. A.; Gavrilov, V.; Geng, W.; Gerber, C. E.; Gershtein, Y.; Ginther, G.; Gogota, O.; Golovanov, G.; Grannis, P. D.; Greder, S.; Greenlee, H.; Grenier, G.; Gris, Ph.; Grivaz, J. -F.; Grohsjean, A.; Grünendahl, S.; Grünewald, M. W.; Guillemin, T.; Gutierrez, G.; Gutierrez, P.; Haley, J.; Han, L.; Harder, K.; Harel, A.; Hauptman, J. M.; Hays, J.; Head, T.; Hebbeker, T.; Hedin, D.; Hegab, H.; Heinson, A. P.; Heintz, U.; Hensel, C.; Heredia-De La Cruz, I.; Herner, K.; Hesketh, G.; Hildreth, M. D.; Hirosky, R.; Hoang, T.; Hobbs, J. D.; Hoeneisen, B.; Hogan, J.; Hohlfeld, M.; Holzbauer, J. L.; Howley, I.; Hubacek, Z.; Hynek, V.; Iashvili, I.; Ilchenko, Y.; Illingworth, R.; Ito, A. S.; Jabeen, S.; Jaffré, M.; Jayasinghe, A.; Jeong, M. S.; Jesik, R.; Jiang, P.; Johns, K.; Johnson, E.; Johnson, M.; Jonckheere, A.; Jonsson, P.; Joshi, J.; Jung, A. W.; Juste, A.; Kajfasz, E.; Karmanov, D.; Katsanos, I.; Kaur, M.; Kehoe, R.; Kermiche, S.; Khalatyan, N.; Khanov, A.; Kharchilava, A.; Kharzheev, Y. N.; Kiselevich, I.; Kohli, J. M.; Kozelov, A. V.; Kraus, J.; Kumar, A.; Kupco, A.; Kurča, T.; Kuzmin, V. A.; Lammers, S.; Lebrun, P.; Lee, H. S.; Lee, S. W.; Lee, W. M.; Lei, X.; Lellouch, J.; Li, D.; Li, H.; Li, L.; Li, Q. Z.; Lim, J. K.; Lincoln, D.; Linnemann, J.; Lipaev, V. V.; Lipton, R.; Liu, H.; Liu, Y.; Lobodenko, A.; Lokajicek, M.; Lopes de Sa, R.; Luna-Garcia, R.; Lyon, A. L.; Maciel, A. K. A.; Madar, R.; Magaña-Villalba, R.; Malik, S.; Malyshev, V. L.; Mansour, J.; Martínez-Ortega, J.; McCarthy, R.; McGivern, C. L.; Meijer, M. M.; Melnitchouk, A.; Menezes, D.; Mercadante, P. G.; Merkin, M.; Meyer, A.; Meyer, J.; Miconi, F.; Mondal, N. K.; Mulhearn, M.; Nagy, E.; Narain, M.; Nayyar, R.; Neal, H. A.; Negret, J. P.; Neustroev, P.; Nguyen, H. T.; Nunnemann, T.; Orduna, J.; Osman, N.; Osta, J.; Pal, A.; Parashar, N.; Parihar, V.; Park, S. K.; Partridge, R.; Parua, N.; Patwa, A.; Penning, B.; Perfilov, M.; Peters, Y.; Petridis, K.; Petrillo, G.; Pétroff, P.; Pleier, M. -A.; Podstavkov, V. M.; Popov, A. V.; Prewitt, M.; Price, D.; Prokopenko, N.; Qian, J.; Quadt, A.; Quinn, B.; Ratoff, P. N.; Razumov, I.; Ripp-Baudot, I.; Rizatdinova, F.; Rominsky, M.; Ross, A.; Royon, C.; Rubinov, P.; Ruchti, R.; Sajot, G.; Sánchez-Hernández, A.; Sanders, M. P.; Santos, A. S.; Savage, G.; Savitskyi, M.; Sawyer, L.; Scanlon, T.; Schamberger, R. D.; Scheglov, Y.; Schellman, H.; Schwanenberger, C.; Schwienhorst, R.; Sekaric, J.; Severini, H.; Shabalina, E.; Shary, V.; Shaw, S.; Shchukin, A. A.; Simak, V.; Skubic, P.; Slattery, P.; Smirnov, D.; Snow, G. R.; Snow, J.; Snyder, S.; Söldner-Rembold, S.; Sonnenschein, L.; Soustruznik, K.; Stark, J.; Stoyanova, D. A.; Strauss, M.; Suter, L.; Svoisky, P.; Titov, M.; Tokmenin, V. V.; Tsai, Y. -T.; Tsybychev, D.; Tuchming, B.; Tully, C.; Uvarov, L.; Uvarov, S.; Uzunyan, S.; Van Kooten, R.; van Leeuwen, W. M.; Varelas, N.; Varnes, E. W.; Vasilyev, I. A.; Verkheev, A. Y.; Vertogradov, L. S.; Verzocchi, M.; Vesterinen, M.; Vilanova, D.; Vokac, P.; Wahl, H. D.; Wang, M. H. L. S.; Warchol, J.; Watts, G.; Wayne, M.; Weichert, J.; Welty-Rieger, L.; Williams, M. R. J.; Wilson, G. W.; Wobisch, M.; Wood, D. R.; Wyatt, T. R.; Xie, Y.; Yamada, R.; Yang, S.; Yasuda, T.; Yatsunenko, Y. A.; Ye, W.; Ye, Z.

    2014-12-01

    We measure the direct mi>Cmi>mi>P>-violating parameter mi>Ami>mi>Cmi>mi>Pmi> for the decay of the charged charm meson, mi>Dmi>+mi>Kmi>-mi>πmi>+mi>πmi>+ (and charge conjugate), using the full 10.4 mi>fbmi>-1 sample of mi>p>mi>p>¯ collisions at mi>smi>=1.96 mi>TeVmi> collected by the D0 detector at the Fermilab Tevatron collider. We extract the raw reconstructed charge asymmetry by fitting the invariant mass distributions for the sum and difference of charge-specific samples. This quantity is then corrected for detector-related asymmetries using data-driven methods and for possible physics asymmetries (from mi>B>mi>D

  20. Women in the Hotel and Catering Industry.

    ERIC Educational Resources Information Center

    Hotel and Catering Training Board, Wembley (England).

    A study of the employment of women in the hotel and catering industry indicated that the industry employs nearly 17 percent of the entire paid female work force in the United Kingdom. Women constitute 75 percent of the industry's work force, and 47 percent of its managers are women. Women's position in the industry is characterized by their…

  1. MiR-1246

    PubMed Central

    Liao, Jun-Ming; Zhou, Xiang; Zhang, Yu; Lu, Hua

    2012-01-01

    Since the discovery of miRNAs, a number of miRNAs have been identified as p53’s transcriptional targets. Most of them are involved in regulation of the known p53 functions, such as cell cycle, apoptosis and senescence. Our recent study revealed miR-1246 as a novel target of p53 and its analogs p63 and p73 to suppress the expression of DYRK1A and consequently activate NFAT, both of which are associated with Down syndrome and possibly with tumorigenesis. This finding suggests that miR-1246 might serve as a likely link of the p53 family with Down syndrome. Here, we provide some prospective views on the potential role of the p53 family in Down syndrome via miR-1246 and propose a new p53-miR-1246-DYRK1A-NFAT pathway in cancer. PMID:22751441

  2. Viral miRNAs.

    PubMed

    Plaisance-Bonstaff, Karlie; Renne, Rolf

    2011-01-01

    Since 2004, more than 200 microRNAs (miRNAs) have been discovered in double-stranded DNA viruses, mainly herpesviruses and polyomaviruses (Nucleic Acids Res 32:D109-D111, 2004). miRNAs are short 22  ±  3 nt RNA molecules that posttranscriptionally regulate gene expression by binding to 3'-untranslated regions (3'UTR) of target mRNAs, thereby inducing translational silencing and/or transcript degradation (Nature 431:350-355, 2004; Cell 116:281-297, 2004). Since miRNAs require only limited complementarity for binding, miRNA targets are difficult to determine (Mol Cell 27:91-105, 2007). To date, targets have only been experimentally verified for relatively few viral miRNAs, which either target viral or host cellular gene expression: For example, SV40 and related polyomaviruses encode miRNAs which target viral large T antigen expression (Nature 435:682-686, 2005; J Virol 79:13094-13104, 2005; Virology 383:183-187, 2009; J Virol 82:9823-9828, 2008) and miRNAs of α-, β-, and γ-herpesviruses have been implicated in regulating the transition from latent to lytic gene expression, a key step in the herpesvirus life cycle. Viral miRNAs have also been shown to target various host cellular genes. Although this field is just beginning to unravel the multiple roles of viral miRNA in biology and pathogenesis, the current data strongly suggest that virally encoded miRNAs are able to regulate fundamental biological processes such as immune recognition, promotion of cell survival, angiogenesis, proliferation, and cell differentiation. This chapter aims to summarize our current knowledge of viral miRNAs, their targets and function, and the challenges lying ahead to decipher their role in viral biology, pathogenesis, and for γ-herepsvirus-encoded miRNAs, potentially tumorigenesis. PMID:21431678

  3. Legionellosis Outbreak Associated With a Hotel Fountain

    PubMed Central

    Smith, Shamika S.; Ritger, Kathy; Samala, Usha; Black, Stephanie R.; Okodua, Margaret; Miller, Loretta; Kozak-Muiznieks, Natalia A.; Hicks, Lauri A.; Steinheimer, Craig; Ewaidah, Saadeh; Presser, Lance; Siston, Alicia M.

    2015-01-01

    Background. In August 2012, the Chicago Department of Public Health (CDPH) was notified of acute respiratory illness, including 1 fatality, among a group of meeting attendees who stayed at a Chicago hotel during July 30–August 3, 2012. Suspecting Legionnaires' disease (LD), CDPH advised the hotel to close their swimming pool, spa, and decorative lobby fountain and began an investigation. Methods. Case finding included notification of individuals potentially exposed during July 16–August 15, 2012. Individuals were interviewed using a standardized questionnaire. An environmental assessment was performed. Results. One hundred fourteen cases were identified: 11 confirmed LD, 29 suspect LD, and 74 Pontiac fever cases. Illness onsets occurred July 21–August 22, 2012. Median age was 48 years (range, 22–82 years), 64% were male, 59% sought medical care (15 hospitalizations), and 3 died. Relative risks for hotel exposures revealed that persons who spent time near the decorative fountain or bar, both located in the lobby were respectively 2.13 (95%, 1.64–2.77) and 1.25 (95% CI, 1.09–1.44) times more likely to become ill than those who did not. Legionella pneumophila serogroup 1 was isolated from samples collected from the fountain, spa, and women's locker room fixtures. Legionella pneumophila serogroup 1 environmental isolates and a clinical isolate had matching sequence-based types. Hotel maintenance records lacked a record of regular cleaning and disinfection of the fountain. Conclusions. Environmental testing identified Legionella in the hotel's potable water system. Epidemiologic and laboratory data indicated the decorative fountain as the source. Poor fountain maintenance likely created favorable conditions for Legionella overgrowth. PMID:26716104

  4. Chronic disease risk factors among hotel workers

    PubMed Central

    Gawde, Nilesh Chandrakant; Kurlikar, Prashika R.

    2016-01-01

    Context: Non-communicable diseases have emerged as a global health issue. Role of occupation in pathogenesis of non-communicable diseases has not been explored much especially in the hospitality industry. Aims: Objectives of this study include finding risk factor prevalence among hotel workers and studying relationship between occupational group and chronic disease risk factors chiefly high body mass index. Settings and Design: A cross-sectional study was conducted among non-managerial employees from classified hotels in India. Materials and Methods: The study participants self-administered pre-designed pilot-tested questionnaires. Statistical analysis used: The risk factor prevalence rates were expressed as percentages. Chi-square test was used for bi-variate analysis. Overweight was chosen as ‘outcome’ variable of interest and binary multi-logistic regression analysis was used to identify determinants. Results: The prevalence rates of tobacco use, alcohol use, inadequate physical activity and inadequate intake of fruits and vegetables were 32%, 49%, 24% and 92% respectively among hotel employees. Tobacco use was significantly common among those in food preparation and service, alcohol use among those in food service and security and leisure time physical activity among front office workers. More than two-fifths (42.7%) were overweight. Among the hotel workers, those employed in food preparation and security had higher odds of 1.650 (CI: 1.025 – 2.655) and 3.245 (CI: 1.296 – 8.129) respectively of being overweight. Conclusions: Prevalence of chronic disease risk factors is high among hotel workers. Risk of overweight is significantly high in food preparation and security departments and workplace interventions are necessary to address these risks PMID:27390474

  5. HVAC systems and energy conservation in hotels

    SciTech Connect

    Wagner, J.R.

    1986-01-01

    This paper discusses the effect that the design of the basic HVAC has on the relative success of energy conservation efforts in hotels. The unusual nature of a hotel is explained along with the impact that it has on the HVAC system. The paper stresses the practical considerations which must be made by the HVAC system designer when he designs the guest rooms, public areas, back-of-the-house areas, temperature control system, and energy management system. The advantages of providing separate air-handling systems are presented. The benefits of line voltage electric controls for guest room fan coil units are explained. General recommendations for arrangement of ventilation systems and possible opportunities for heat recovery are included.

  6. Chinese hotel general managers' perspectives on energy-saving practices

    NASA Astrophysics Data System (ADS)

    Zhu, Yidan

    As hotels' concern about sustainability and budget-control is growing steadily, energy-saving issues have become one of the important management concerns hospitality industry face. By executing proper energy-saving practices, previous scholars believed that hotel operation costs can decrease dramatically. Moreover, they believed that conducting energy-saving practices may eventually help the hotel to gain other benefits such as an improved reputation and stronger competitive advantage. The energy-saving issue also has become a critical management problem for the hotel industry in China. Previous research has not investigated energy-saving in China's hotel segment. To achieve a better understanding of the importance of energy-saving, this document attempts to present some insights into China's energy-saving practices in the tourist accommodations sector. Results of the study show the Chinese general managers' attitudes toward energy-saving issues and the differences among the diverse hotel managers who responded to the study. Study results indicate that in China, most of the hotels' energy bills decrease due to the implementation of energy-saving equipments. General managers of hotels in operation for a shorter period of time are typically responsible for making decisions about energy-saving issues; older hotels are used to choosing corporate level concerning to this issue. Larger Chinese hotels generally have official energy-saving usage training sessions for employees, but smaller Chinese hotels sometimes overlook the importance of employee training. The study also found that for the Chinese hospitality industry, energy-saving practices related to electricity are the most efficient and common way to save energy, but older hotels also should pay attention to other ways of saving energy such as water conservation or heating/cooling system.

  7. Renovation And Modernization Of Hotel Buildings - Case Studies In Silesia

    NASA Astrophysics Data System (ADS)

    Bradecki, Tomasz; Uherek-Bradecka, Barbara

    2015-12-01

    The cultural heritage of Silesia has different backgrounds and is often characterized by difficult to assess values. There is doubt as to whether some of the existing buildings should be modernized. Since 2000, an increase in the amount of investments in hotel buildings and conference venues in Poland has been observed. The functions and roles of hotels within a city have also changed. The paper presents examples of original projects and realizations of hotel buildings in Silesia. A discussion was also held regarding the issue of adapting and modernizing hotel buildings.

  8. Hotel & Food Service Industries. Workforce & Workplace Literacy Series.

    ERIC Educational Resources Information Center

    BCL Brief, 1992

    1992-01-01

    This brief gives an overview of the topic of workplace literacy for the hotel and food service industries and lists program contacts. The following organizations operate employee basic skills programs for hotel and food service employees, provide technical assistance, or operate grant programs: Essential Skills Resource Center; Language Training…

  9. Traveler's encounter with nymphs in a hotel bed

    PubMed Central

    Sandlund, Johanna; Banaei, Niaz

    2014-01-01

    This case illustrates skin lesions in a traveler staying in a hotel bed infested with tics. Although infestation of hotels with bedbugs belonging to the Cimex genus is a growing problem worldwide, tick infestation has never been reported before. PMID:26839772

  10. Management Trainees in the Hotel Industry: What Do Managers Expect?

    ERIC Educational Resources Information Center

    Baum, Tom

    1991-01-01

    Comparison of a survey of 118 United Kingdom hotel managers (53 percent response) with a similar study of 75 U.S. managers found key differences reflecting the impact of cultures on management expectations. Significant similarities support development of an internationally transferable core curriculum for hotel management. (SK)

  11. VIEW LOOKING WEST TOWARD RESERVOIR HILL. THE SPRR HOTEL WAS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW LOOKING WEST TOWARD RESERVOIR HILL. THE SPRR HOTEL WAS LOCATED IN THE STRIPED AREA AT THE BOTTOM OF THE IMAGE, AND THE TRACK RAN BETWEEN THE HILL AND THE HOTEL. - Southern Pacific Railroad Water Settling Reservoir, Yuma Crossing, south bank of Colorado River at foot of Madison Avenue, Yuma, Yuma County, AZ

  12. New Employment Forecasts. Hotel and Catering Industry 1988-1993.

    ERIC Educational Resources Information Center

    Measurement for Management Decision, Ltd., London (England).

    Econometric forecasting models were used to forecast employment levels in the hotel and catering industry in Great Britain through 1993 under several different forecasting scenarios. The growth in employment in the hotel and catering industry over the next 5 years is likely to be broadly based, both across income levels of domestic consumers,…

  13. Changing Occupational Profiles in the Hotel Industry: Case Studies in France, Italy and Spain. Synthesis Report.

    ERIC Educational Resources Information Center

    Gatti, Mario; Grazia Mereu, Maria; Tagliaferro, Claudio

    Changing occupational profiles in the hotel industry in France, Italy, and Spain were examined in case studies that included interviews with hotel managers, human resource managers, and individuals employed in hotel occupations identified as new or entailing new skills. The study focused on the following topics: (1) changes in the hotel industry…

  14. Strategic planning for hotel operations: The Ritz-Carlton Hotel Company (Part I).

    PubMed

    Shriver, S J

    1993-01-01

    The Ritz-Carlton Hotel Company won the Malcolm Baldridge National Quality Award in 1992. One key to its success is its strategic planning process. This two-part article reviews the Ritz-Carlton's approach to strategic planning. In particular, it describes (1) the role of senior leadership in the planning process and (2) the specific activities that are associated with plan development and implementation. PMID:10131010

  15. MI1AENG1

    Atmospheric Science Data Center

    2014-09-03

    MI1AENG1 MISR Level 1A Engineering Data File Type 1: Reformatted Annotated Level 1A product for the camera engineering data, which represents indicators of sampled measurements. ... Status Production Report Read Software Files :  Data Product Specification Versioning ...

  16. MI1ANAV

    Atmospheric Science Data Center

    2014-09-03

    MI1ANAV MISR Level 1A Navigation Data: Reformatted Annotated Level 1A Product for the Navigation Data, which contains samples of the Terra Platform position and ... Specification Versioning History:  Engineering, Navigation SCAR-B Block:  SCAR-B ...

  17. Occupational Programs for the Restaurant/Hotel Business

    ERIC Educational Resources Information Center

    Hoenninger, Ronald W.; Riegel, Carl D.

    1978-01-01

    Describes the development of a Hotel and Restaurant Management Program, designed to provide career training, develop educational opportunities, and provide a forum through which the continuing education needs of the local hospitality industry could be assessed and evaluated. (TP)

  18. 88. Paso Del Norte Hotel, 115117 South El Paso St., ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    88. Paso Del Norte Hotel, 115-117 South El Paso St., south facade, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  19. 91. Paso Del Norte Hotel, 115117 South El Paso St., ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    91. Paso Del Norte Hotel, 115-117 South El Paso St., east facade, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  20. PASO DEL NORTE HOTEL, 115117 S. EL PASO ST., SOUTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PASO DEL NORTE HOTEL, 115-117 S. EL PASO ST., SOUTH AND EAST FACADES, WEST SIDE OF STREET - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  1. 92. Paso Del Norte Hotel, 115117 South El Paso St., ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    92. Paso Del Norte Hotel, 115-117 South El Paso St., interior, lobby rotunda - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  2. PASO DEL NORTE HOTEL, 115117 S. EL PASO ST., EAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PASO DEL NORTE HOTEL, 115-117 S. EL PASO ST., EAST FACADE, WEST SIDE OF STREET - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  3. 9. South El Paso St., 203205 (Hotel Fisher), east and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. South El Paso St., 203-205 (Hotel Fisher), east and north facades, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  4. 7. FOURTH FLOOR, DETAIL OF HOTEL SOAP LINE TO WEST: ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. FOURTH FLOOR, DETAIL OF HOTEL SOAP LINE TO WEST: FERGUSON & HAAS AUTOMATIC WRAPPING MACHINE INSTALLED BY 1929 - Colgate & Company Jersey City Plant, Building No. B-15, 90-96 Greene Street, Jersey City, Hudson County, NJ

  5. 5. FOURTH FLOOR, HOTEL SOAP LINES TO NORTHWEST: PRESS (LEFT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. FOURTH FLOOR, HOTEL SOAP LINES TO NORTHWEST: PRESS (LEFT CENTER), MANUAL CUTTERS (CENTER, RIGHT CENTER) - Colgate & Company Jersey City Plant, Building No. B-15, 90-96 Greene Street, Jersey City, Hudson County, NJ

  6. PERSPECTIVE VIEW OF THE C. 1893 HOTEL JULIAN, LOCATED AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PERSPECTIVE VIEW OF THE C. 1893 HOTEL JULIAN, LOCATED AT 103-107 SECOND STREET, VIEW LOOKING SOUTHEAST. - Corvallis Downtown Historic District, Bounded by First & Sixth Streets, Van Buren & Western Avenues, Corvallis, Benton County, OR

  7. Research on the Hotel Image Based on the Detail Service

    NASA Astrophysics Data System (ADS)

    Li, Ban; Shenghua, Zheng; He, Yi

    Detail service management, initially developed as marketing programs to enhance customer loyalty, has now become an important part of customer relation strategy. This paper analyzes the critical factors of detail service and its influence on the hotel image. We establish the theoretical model of influencing factors on hotel image and propose corresponding hypotheses. We use applying statistical method to test and verify the above-mentioned hypotheses. This paper provides a foundation for further study of detail service design and planning issues.

  8. Spinning Reserve from Hotel Air Conditioning Load - SHORT VERSION

    SciTech Connect

    Kueck, John D; Kirby, Brendan J

    2008-01-01

    Even though preliminary tests were not conducted during times of highest system or hotel loading during the summer, they showed that hotel load can be curtailed by 22 to 37 percent depending on the outdoor temperature and time of day. Full response occurred in 12 to 60 seconds from when the system operator's command to shed load was issued and the load drop was very rapid.

  9. miRTargetLink—miRNAs, Genes and Interaction Networks

    PubMed Central

    Hamberg, Maarten; Backes, Christina; Fehlmann, Tobias; Hart, Martin; Meder, Benjamin; Meese, Eckart; Keller, Andreas

    2016-01-01

    Information on miRNA targeting genes is growing rapidly. For high-throughput experiments, but also for targeted analyses of few genes or miRNAs, easy analysis with concise representation of results facilitates the work of life scientists. We developed miRTargetLink, a tool for automating respective analysis procedures that are frequently applied. Input of the web-based solution is either a single gene or single miRNA, but also sets of genes or miRNAs, can be entered. Validated and predicted targets are extracted from databases and an interaction network is presented. Users can select whether predicted targets, experimentally validated targets with strong or weak evidence, or combinations of those are considered. Central genes or miRNAs are highlighted and users can navigate through the network interactively. To discover the most relevant biochemical processes influenced by the target network, gene set analysis and miRNA set analysis are integrated. As a showcase for miRTargetLink, we analyze targets of five cardiac miRNAs. miRTargetLink is freely available without restrictions at www.ccb.uni-saarland.de/mirtargetlink. PMID:27089332

  10. Legionella Contamination in Hot Water of Italian Hotels

    PubMed Central

    Borella, Paola; Montagna, Maria Teresa; Stampi, Serena; Stancanelli, Giovanna; Romano-Spica, Vincenzo; Triassi, Maria; Marchesi, Isabella; Bargellini, Annalisa; Tatò, Daniela; Napoli, Christian; Zanetti, Franca; Leoni, Erica; Moro, Matteo; Scaltriti, Stefania; Ribera D'Alcalà, Gabriella; Santarpia, Rosalba; Boccia, Stefania

    2005-01-01

    A cross-sectional multicenter survey of Italian hotels was conducted to investigate Legionella spp. contamination of hot water. Chemical parameters (hardness, free chlorine concentration, and trace element concentrations), water systems, and building characteristics were evaluated to study risk factors for colonization. The hot water systems of Italian hotels were strongly colonized by Legionella; 75% of the buildings examined and 60% of the water samples were contaminated, mainly at levels of ≥103 CFU liter−1, and Legionella pneumophila was the most frequently isolated species (87%). L. pneumophila serogroup 1 was isolated from 45.8% of the contaminated sites and from 32.5% of the hotels examined. When a multivariate logistic model was used, only hotel age was associated with contamination, but the risk factors differed depending on the contaminating species and serogroup. Soft water with higher chlorine levels and higher temperatures were associated with L. pneumophila serogroup 1 colonization, whereas the opposite was observed for serogroups 2 to 14. In conclusion, Italian hotels, particularly those located in old buildings, represent a major source of risk for Legionnaires' disease due to the high frequency of Legionella contamination, high germ concentration, and major L. pneumophila serogroup 1 colonization. The possible role of chlorine in favoring the survival of Legionella species is discussed. PMID:16204491

  11. Employment Prospects in the Hotel and Catering Trade: A Franco-American Comparison.

    ERIC Educational Resources Information Center

    Meriot, Sylvie-Anne

    2000-01-01

    In both France and the United States, restaurants remain the hotel and catering sector's main employer. In France, 25% of hotel and catering personnel are self-employers versus fewer than 5% in the United States; however, the growth of hotel and restaurant chains in France may eventually limit opportunities for creating an independent activity.…

  12. A large hotel outbreak of Norwalk-like virus gastroenteritis among three groups of guests and hotel employees in Virginia.

    PubMed Central

    Love, S. S.; Jiang, X.; Barrett, E.; Farkas, T.; Kelly, S.

    2002-01-01

    A large outbreak of acute gastroenteritis occurred among three different groups of guests and the employees of a Virginia hotel within a 2-week period in November 2000. At least 76 of the hotel's guests and 40 hotel employees had acute gastroenteritis during this period. All tested ill persons were infected with the same strain of Norwalk-like virus, as shown by cloning and sequencing of virus detected in stool specimens from the three guest groups and the employees. Epidemiologic investigation suggested food as the probable source for the guests. Most of the employees, including those sick, did not eat in the hotel, suggesting that environmental contamination and person-to-person transmission could have contributed to the outbreak. The disease continued to spread in the hotel, passing from one guest group to another, by food, environmental contamination, and/or by person-to-person transmission through infected employees and guests. The study describes procedures implemented to control the outbreak and makes recommendations for future outbreak control. PMID:12211579

  13. Occupancy sensors for HVAC gaining in hotel industry

    SciTech Connect

    Ladd, C.

    1985-12-02

    The hotel industry is overcoming its skepticism as occupancy sensors with built-in thermistors to control heating, ventilating, and air conditioning (HVAC) units demonstrate their ability to cut energy costs as much as 30%. Despite the successful demonstrations and acceptance by Holiday Inn, some hotel managers of other chains continue to resist. Occupancy sensors have either ultrasonic or infrared signals, but differ from lighting control devices by also having internal thermistors and remote door switches. This allows the rooms to reach comfort levels only when the guest is present since occupants are only minimally affected if temperatures are modified during unoccupied periods. The system works best for roadside-type motels rather than convention hotels, where occupants are in and out of their rooms.

  14. Space-Hotel Early Bird - Visions for a Commercial Space Hotel

    NASA Astrophysics Data System (ADS)

    Amekrane, R.; Holze, C.; Apel, U.

    2002-01-01

    rachid.amekrane@astrium-space.com/Fax: +49 421 539-24801, cholze@zarm.uni-bremen.de/Fax: +49 421 218-7473, The International Space Station was planed for research purposes. In 2001 the first private man, Denis Tito,visited the ISS and the second private man, Mark Shuttleworth is following him. The gate towards the commercial utilization of manned space flight has been pushed open. Space pioneers as Wernher von Braun and Sir Arthur C. Clarke had the dream that one day a space station in earth orbit will host tourists. It is evident that the ISS is not designed to host tourists. Therefore the dream of the pioneers is still open. By asking the question "how should a space station should look like to host tourists?", the German Aerospace Society DGLR e.V. organized a contest under the patronage of Mr. Joerg Feustel-Buechl, the Director of Manned Spaceflight and Microgravity, European Space Agency (ESA) in April 2001. Because the definition and design of living space is the content of architecture the approach was to gather new ideas from young architects in cooperation with space experts. This contest was directed at students of architecture and the task set was to design a hotel for the earth orbit and to accommodate 220 guests. The contest got the name "Early Bird - Visions of a Space Hotel". The results and models of the student's work were shown in an exhibition in Hamburg/Germany, which was open to the public from September 19th till October 20th 2001. During the summer term of 2001 seventeen designs were completed. Having specialists, as volunteers, in the field of space in charge meant that it could be ensured that the designs reflected a certain possibility of being able to be realized. Within this interdisciplinary project both parties learned from each other. The 17 different designs were focused on the expectations and needs of a future space tourist. The designs are for sure not feasible today, but the designs are in that sense realistic that they could be

  15. Spinning Reserve From Hotel Load Response: Initial Progress

    SciTech Connect

    Kueck, John D; Kirby, Brendan J

    2008-11-01

    This project was motivated by the fundamental match between hotel space conditioning load response capability and power system contingency response needs. As power system costs rise and capacity is strained demand response can provide a significant system reliability benefit at a potentially attractive cost. At ORNL s suggestion, Digital Solutions Inc. adapted its hotel air conditioning control technology to supply power system spinning reserve. This energy saving technology is primarily designed to provide the hotel operator with the ability to control individual room temperature set-points based upon occupancy (25% to 50% energy savings based on an earlier study [Kirby and Ally, 2002]). DSI added instantaneous local load shedding capability in response to power system frequency and centrally dispatched load shedding capability in response to power system operator command. The 162 room Music Road Hotel in Pigeon Forge Tennessee agreed to host the spinning reserve test. The Tennessee Valley Authority supplied real-time metering equipment in the form of an internet connected Dranetz-BMI power quality meter and monitoring expertise to record total hotel load during both normal operations and test results. The Sevier County Electric System installed the metering. Preliminary testing showed that hotel load can be curtailed by 22% to 37% depending on the outdoor temperature and the time of day. These results are prior to implementing control over the common area air conditioning loads. Testing was also not at times of highest system or hotel loading. Full response occurred in 12 to 60 seconds from when the system operator s command to shed load was issued. The load drop was very rapid, essentially as fast as the 2 second metering could detect, with all units responding essentially simultaneously. Load restoration was ramped back in over several minutes. The restoration ramp can be adjusted to the power system needs. Frequency response testing was not completed. Initial

  16. Comprehensive On-Site Services in an Emergency Relocation Hotel.

    ERIC Educational Resources Information Center

    Krim, Alaine S.

    This paper describes a comprehensive program to provide on-site services to poor families following emergency relocation caused by various catastrophes. When the report was prepared, the program involved 75 to 125 families (primarily black and Spanish-speaking) temporarily living in a hotel. The program's objectives were to (1) develop coordinated…

  17. Two Basic Programs to Compute Hotelling's T-Square Statistic.

    ERIC Educational Resources Information Center

    Powers, Stephen; Jones, Patricia B.

    1986-01-01

    This paper describes two BASIC computer programs that calculate Hotelling's T-square either for one sample or for two samples. Output of the progams includes the Mahalanobis distance D-square, the F ratio associated with T-square, and its probability level. (Author)

  18. Hotel found liable in firing of HIV-positive bartender.

    PubMed

    1997-03-01

    A Federal jury in the court of U.S. Magistrate Judge Leslie G. Foschio of the Western District of New York found the Buffalo [name removed] liable for more than $630,000 in damages to bartender [name removed], who was fired because of his HIV infection. The [name removed] unsuccessfully argued that [name removed] was terminated because of unfavorable performance reviews. The plaintiffs claimed that the [name removed] issued false citations for infractions of hotel rules and that these citations were only issued after hotel management determined that [name removed] was HIV-positive. Testimony showed that all relevant decision-making personnel at the hotel were aware that [name removed] was HIV-positive. The hotel failed to show that all but the personnel manager had a legitimate need to know about [name removed]'s HIV status. The jury awarded [name removed] $1,439,000 in damages. Foschio lowered the amount to $637,388 in damages and $133,705 in attorneys' fees. PMID:11364135

  19. Residents' Coping Strategies in an Extended-Stay Hotel Home

    ERIC Educational Resources Information Center

    Lewinson, Terri

    2010-01-01

    Some families live in extended-stay hotels as a solution after housing displacement. This temporary accommodation provides a furnished home environment with resources such as a kitchenette, bed, heating/air conditioning, and room services with one payment that can be made weekly or monthly without a credit check or rent deposit. Despite these…

  20. Competency Needs in Irish Hotels: Employer and Graduate Perspectives

    ERIC Educational Resources Information Center

    Nolan, Ciara; Conway, Edel; Farrell, Tara; Monks, Kathy

    2010-01-01

    Purpose: The purpose of this study is to investigate hotel industry employers' expectations of, and satisfaction with, graduate competencies in comparison with graduate perceptions of what is required for their roles and their satisfaction with how well their education experience prepared them. Design/methodology/approach: The research involved a…

  1. Women's Path to Management in the Hotel and Catering Industry.

    ERIC Educational Resources Information Center

    Hotel and Catering Training Board, Wembley (England).

    A study examined the employment patterns of women working in the United Kingdom's hotel and catering industry and the opportunities for vocational education that would lead to careers in management. Sixty-four women from different sectors of the industry were interviewed (including women in senior and middle management and some still looking to be…

  2. Solar-Cooled Hotel in the Virgin Islands

    NASA Technical Reports Server (NTRS)

    Harber, H.

    1982-01-01

    Performance of solar cooling system is described in 21-page report. System provides cooling for public areas including ball rooms, restaurant, lounge, lobby and shops. Chilled water from solar-cooling system is also used to cool hot water from hotel's desalinization plant.

  3. ESL for Hotel/Hospitality Industry. Level: Beginner.

    ERIC Educational Resources Information Center

    Western Suffolk County Board of Cooperative Educational Services, Northport, NY.

    This document contains eight lesson plans for a beginning course in work-related English for non-English or limited-English speaking entry-level employees in the hotel and hospitality industry. Course objectives include the following: helping participants understand and use job-specific vocabulary; receive and understand job-related instructions;…

  4. Paperwork Plus: Literacy Materials for the Service Industry. Hotel Edition.

    ERIC Educational Resources Information Center

    Bond, Judith; McGill, Teresa

    The instructional materials are intended for use in teaching vocational English and English literacy to limited-English-speaking personnel in the hotel industry. They are designed for learners at three instructional levels, and address job-specific literacy tasks. An introductory section describes the materials and offers suggestions for…

  5. Understanding Work-Family Spillover in Hotel Managers.

    PubMed

    Lawson, Katie M; Davis, Kelly D; Crouter, Ann C; O'Neill, John W

    2013-06-01

    The present study examined the experience of work-family spillover among 586 hotel managers (HMs) working in 50 full-service hotels throughout the U.S. Work-family spillover occurs when behaviors, moods, stresses, and emotions from work spill over into family. We first investigated which hotel managers were more likely to experience spillover and stressful work conditions based on their life circumstances (gender, parental status, age, decision-making latitude at work). Second, we investigated which work conditions (hours worked per week, organizational time expectations, emotional labor, and permeable boundaries) predicted more work-family spillover. Women, employees without children at home, and younger adults experienced the highest levels of negative work-family spillover. Work conditions, particularly organizational time expectations, put HMs at risk for experiencing more negative and less positive work-family spillover. The results provide evidence that modifying certain work conditions in the hotel industry may be helpful in improving the quality of HMs' jobs and retention. PMID:23888092

  6. 87. Paso Del Norte Hotel, 115117 South El Paso St., ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    87. Paso Del Norte Hotel, 115-117 South El Paso St., south and east facades, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  7. 89. Paso Del Norte Hotel, 115117 South El Paso St., ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    89. Paso Del Norte Hotel, 115-117 South El Paso St., detail view of roof, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  8. The Crystal Hotel: A Microfluidic Approach to Biomimetic Crystallization.

    PubMed

    Gong, Xiuqing; Wang, Yun-Wei; Ihli, Johannes; Kim, Yi-Yeoun; Li, Shunbo; Walshaw, Richard; Chen, Li; Meldrum, Fiona C

    2015-12-01

    A "crystal hotel" microfluidic device that allows crystal growth in confined volumes to be studied in situ is used to produce large calcite single crystals with predefined crystallographic orientation, microstructure, and shape by control of the detailed physical environment, flow, and surface chemistry. This general approach can be extended to form technologically important, nanopatterned single crystals. PMID:26479157

  9. 12. DETAIL OF REMOVED PLODDER (UPPER HALF) FROM HOTEL SOAP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. DETAIL OF REMOVED PLODDER (UPPER HALF) FROM HOTEL SOAP LINE No. 6 STORED IN G BLOCK (HAER No. NJ-71-NN) - Colgate & Company Jersey City Plant, Building No. B-14, 54-58 Grand Street, Jersey City, Hudson County, NJ

  10. 13. DETAIL OF REMOVED PLODDER (LOWER HALF) FROM HOTEL SOAP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. DETAIL OF REMOVED PLODDER (LOWER HALF) FROM HOTEL SOAP LINE No. 6 STORED IN G BLOCK (HAER No. NJ-71-NN) - Colgate & Company Jersey City Plant, Building No. B-14, 54-58 Grand Street, Jersey City, Hudson County, NJ

  11. 6. FOURTH FLOOR, DETAIL OF HOTEL SOAP LINE TO NORTH: ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. FOURTH FLOOR, DETAIL OF HOTEL SOAP LINE TO NORTH: AMERICAN CAR & FOUNDRY COMPANY MANUAL SOAP CUTTER INSTALLED 1932 (FOREGROUND); CONVEYORS; AND R.A. JONES & COMPANY HORIZONTAL PRESS INSTALLED 1931 (BACKGROUND) - Colgate & Company Jersey City Plant, Building No. B-15, 90-96 Greene Street, Jersey City, Hudson County, NJ

  12. Understanding Work-Family Spillover in Hotel Managers

    PubMed Central

    Lawson, Katie M.; Davis, Kelly D.; Crouter, Ann C.; O’Neill, John W.

    2013-01-01

    The present study examined the experience of work-family spillover among 586 hotel managers (HMs) working in 50 full-service hotels throughout the U.S. Work-family spillover occurs when behaviors, moods, stresses, and emotions from work spill over into family. We first investigated which hotel managers were more likely to experience spillover and stressful work conditions based on their life circumstances (gender, parental status, age, decision-making latitude at work). Second, we investigated which work conditions (hours worked per week, organizational time expectations, emotional labor, and permeable boundaries) predicted more work-family spillover. Women, employees without children at home, and younger adults experienced the highest levels of negative work-family spillover. Work conditions, particularly organizational time expectations, put HMs at risk for experiencing more negative and less positive work-family spillover. The results provide evidence that modifying certain work conditions in the hotel industry may be helpful in improving the quality of HMs’ jobs and retention. PMID:23888092

  13. Evaluation of internal noise methods for Hotelling observer models

    SciTech Connect

    Zhang Yani; Pham, Binh T.; Eckstein, Miguel P.

    2007-08-15

    The inclusion of internal noise in model observers is a common method to allow for quantitative comparisons between human and model observer performance in visual detection tasks. In this article, we studied two different strategies for inserting internal noise into Hotelling model observers. In the first strategy, internal noise was added to the output of individual channels: (a) Independent nonuniform channel noise, (b) independent uniform channel noise. In the second strategy, internal noise was added to the decision variable arising from the combination of channel responses. The standard deviation of the zero mean internal noise was either constant or proportional to: (a) the decision variable's standard deviation due to the external noise, (b) the decision variable's variance caused by the external noise, (c) the decision variable magnitude on a trial to trial basis. We tested three model observers: square window Hotelling observer (HO), channelized Hotelling observer (CHO), and Laguerre-Gauss Hotelling observer (LGHO) using a four alternative forced choice (4AFC) signal known exactly but variable task with a simulated signal embedded in real x-ray coronary angiogram backgrounds. The results showed that the internal noise method that led to the best prediction of human performance differed across the studied model observers. The CHO model best predicted human observer performance with the channel internal noise. The HO and LGHO best predicted human observer performance with the decision variable internal noise. The present results might guide researchers with the choice of methods to include internal noise into Hotelling model observers when evaluating and optimizing medical image quality.

  14. miRNAs Related to Skeletal Diseases.

    PubMed

    Seeliger, Claudine; Balmayor, Elizabeth R; van Griensven, Martijn

    2016-09-01

    miRNAs as non-coding, short, double-stranded RNA segments are important for cellular biological functions, such as proliferation, differentiation, and apoptosis. miRNAs mainly contribute to the inhibition of important protein translations through their cleavage or direct repression of target messenger RNAs expressions. In the last decade, miRNAs got in the focus of interest with new publications on miRNAs in the context of different diseases. For many types of cancer or myocardial damage, typical signatures of local or systemically circulating miRNAs have already been described. However, little is known about miRNA expressions and their molecular effect in skeletal diseases. An overview of published studies reporting miRNAs detection linked with skeletal diseases was conducted. All regulated miRNAs were summarized and their molecular interactions were illustrated. This review summarizes the involvement and interaction of miRNAs in different skeletal diseases. Thereby, 59 miRNAs were described to be deregulated in tissue, cells, or in the circulation of osteoarthritis (OA), 23 miRNAs deregulated in osteoporosis, and 107 miRNAs deregulated in osteosarcoma (OS). The molecular influences of miRNAs regarding OA, osteoporosis, and OS were illustrated. Specific miRNA signatures for skeletal diseases are described in the literature. Some overlapped, but also unique ones for each disease exist. These miRNAs may present useful targets for the development of new therapeutic approaches and are candidates for diagnostic evaluations. PMID:27418331

  15. From Hotel to High School: Converting a Residential Hotel into a New Type of Senior High School. Report and Recommendations of the Concourse Plaza High School Planning Committee.

    ERIC Educational Resources Information Center

    Sasserath, Simpson

    This document reports the result of a 5-day meeting held to recommend the structural building adaptations and the curriculum organization necessary to the renovation of Concourse Plaza Hotel into a high school. According to the planning committee, the hotel has many features adaptable to a school, which would permit a meaningful departure from the…

  16. Circulating microRNAs, miR-939, miR-595, miR-519d and miR-494, Identify Cirrhotic Patients with HCC

    PubMed Central

    Fornari, Francesca; Ferracin, Manuela; Trerè, Davide; Milazzo, Maddalena; Marinelli, Sara; Galassi, Marzia; Venerandi, Laura; Pollutri, Daniela; Patrizi, Clarissa; Borghi, Alberto; Foschi, Francesco G.; Stefanini, Giuseppe F.; Negrini, Massimo; Bolondi, Luigi; Gramantieri, Laura

    2015-01-01

    The performance of circulating biomarkers for the diagnosis of hepatocellular carcinoma (HCC) is sub-optimal. In this study we tested circulating microRNAs as biomarkers for HCC in cirrhotic patients by performing a two stage study: a discovery phase conducted by microarray and a validation phase performed by qRT-PCR in an independent series of 118 patients. Beside miRNAs emerged from the discovery phase, miR-21, miR-221, miR-519d were also tested in the validation setting on the basis of literary and tissue findings. Deregulated microRNAs were assayed in HCC-derived cells in the intracellular compartment, cell culture supernatant and exosomal fraction. Serum and tissue microRNA levels were compared in 14 patients surgically treated for HCC. From the discovery study, it emerged that seven circulating microRNAs were differentially expressed in cirrhotic patients with and without HCC. In the validation set, miR-939, miR-595 and miR-519d were shown to differentiate cirrhotic patients with and without HCC. MiR-939 and miR-595 are independent factors for HCC. ROC curves of miR-939, miR-595 and miR-519d displayed that AUC was higher than AFP. An exosomal secretion of miR-519d, miR-21, miR-221 and miR-1228 and a correlation between circulating and tissue levels of miR-519d, miR-494 and miR-21 were found in HCC patients. Therefore, we show that circulating microRNAs deserve attention as non-invasive biomarkers in the diagnostic setting of HCC and that exosomal secretion contributes to discharging a subset of microRNAs into the extracellular compartment. PMID:26509672

  17. How Clean Are Hotel Rooms? Part II: Examining the Concept of Cleanliness Standards.

    PubMed

    Almanza, Barbara A; Kirsch, Katie; Kline, Sheryl Fried; Sirsat, Sujata; Stroia, Olivia; Choi, Jin Kyung; Neal, Jay

    2015-01-01

    Hotel room cleanliness is based on observation and not on microbial assessment even though recent reports suggest that infections may be acquired while staying in hotel rooms. Exploratory research in the first part of the authors' study was conducted to determine if contamination of hotel rooms occurs and whether visual assessments are accurate indicators of hotel room cleanliness. Data suggested the presence of microbial contamination that was not reflective of visual assessments. Unfortunately, no standards exist for interpreting microbiological data and other indicators of cleanliness in hotel rooms. The purpose of the second half of the authors' study was to examine cleanliness standards in other industries to see if they might suggest standards in hotels. Results of the authors' study indicate that standards from other related industries do not provide analogous criteria, but do provide suggestions for further research. PMID:26427263

  18. Evaluation of the channelized Hotelling observer for signal detection in 2D tomographic imaging

    NASA Astrophysics Data System (ADS)

    LaRoque, Samuel J.; Sidky, Emil Y.; Edwards, Darrin C.; Pan, Xiaochuan

    2007-03-01

    Signal detection by the channelized Hotelling (ch-Hotelling) observer is studied for tomographic application by employing a small, tractable 2D model of a computed tomography (CT) system. The primary goal of this manuscript is to develop a practical method for evaluating the ch-Hotelling observer that can generalize to larger 3D cone-beam CT systems. The use of the ch-Hotelling observer for evaluating tomographic image reconstruction algorithms is also demonstrated. For a realistic model for CT, the ch-Hotelling observer can be a good approximation to the ideal observer. The ch-Hotelling observer is applied to both the projection data and the reconstructed images. The difference in signal-to-noise ratio for signal detection in both of these domains provides a metric for evaluating the image reconstruction algorithm.

  19. Winning cancer centre has 'hotel-like' quality.

    PubMed

    Baillie, Jonathan

    2009-04-01

    A "highly effective" three-way partnership between architects Anshen + Allen, the Newcastle upon Tyne Hospitals NHS Foundation Trust, and Laing O'Rourke, has created a non-institutional and welcoming new cancer treatment and renal services centre in Newcastle upon Tyne which, despite the gruelling nature of some of the therapies set to be offered, has a character and feel early users describe as "more like a four-star hotel" than a conventional healthcare facility. Jonathan Baillie reports. PMID:19452798

  20. Work Stress and Well-being in the Hotel Industry.

    PubMed

    O'Neill, John W; Davis, Kelly

    2011-06-01

    Employee stress is a significant issue in the hospitality industry, and it is costly for employers and employees alike. Although addressing and reducing stress is both a noble goal and is capable of resulting in expense reductions for employers, the nature and quantity of hospitality employee stress is not fully understood. The first aim of this study was to identify common work stressors in a sample of 164 managerial and hourly workers employed at 65 different hotels who were each interviewed for eight consecutive days. The two most common stressors were interpersonal tensions at work and overloads (e.g., technology not functioning). The second aim was to determine whether there were differences in the types and frequency of work stressors by job type (i.e., managers v. non-managers), gender, and marital status. Hotel managers reported significantly more stressors than hourly employees. There were no significant differences by gender or marital status. The third aim was to investigate whether the various stressors were linked to hotel employee health and work outcomes. More employee and coworker stressors were linked to more negative physical health symptoms. Also, interpersonal tensions at work were linked to lower job satisfaction and greater turnover intentions. PMID:23794780

  1. The Hotel Payload, plans for the period 2003-2006

    NASA Astrophysics Data System (ADS)

    Hansen, Gudmund; Mikalsen, Per-Arne

    2003-08-01

    The cost and complexity of scientific experiments, carried by traditional sounding rocket payloads, are increasing. At the same time the scientific environment faces declining funding for this basic research. In order to meet the invitation from the science community, Andøya Rocket Range runs a programme for developing a sounding rocket payload, in order to achieve an inexpensive and cost-effective tool for atmosphere research and educational training. The Hotel Payload is a new technological payload concept in the sounding rocket family. By means of standardized mechanical structures and electronics, flexibility in data collection and transmission, roomy vehicles are affordable to most of the scientific research environments as well as for educational training. A complete vehicle - ready for installation of scientific experiments - is offered to the scientists to a fixed price. The fixed price service also includes launch services. This paper describes the Hotel Payload concept and its technology. In addition the three year plan for the development project is discussed. The opportunity of using the Hotel Payload as a platform for a collaborative triangle between research, education and industry is also discussed.

  2. Single-room occupancy hotels: possible solutions and alternatives.

    PubMed

    Foley, D

    1998-09-01

    New York City's Division of AIDS Services and Income Support (DASIS) places clients in economical, commercial residences in one of 33 hotels in the New York City area, termed single-room occupancy (SRO) hotels. There have been many problems with these hotels in terms of safety and health. One problem is how to enforce housing laws when city agencies have had drastic personnel cutbacks, and landlords are not held accountable for repairs and building conditions. Without a strategic plan to supplement SROs with necessary services, and a way to deal with homeless people with AIDS, it will be difficult to redeem the condition of SROs and lessen dependency on them as a long-term solution. The West Side SRO Law Project offers tips on how tenants in SROs can safeguard their rights and document their cases if they feel that their rights have been violated. Included is a resource list for legal help and emergency numbers for the Department of Buildings in New York City and the surrounding boroughs. PMID:11365813

  3. Work Stress and Well-being in the Hotel Industry

    PubMed Central

    O'Neill, John W.; Davis, Kelly

    2013-01-01

    Employee stress is a significant issue in the hospitality industry, and it is costly for employers and employees alike. Although addressing and reducing stress is both a noble goal and is capable of resulting in expense reductions for employers, the nature and quantity of hospitality employee stress is not fully understood. The first aim of this study was to identify common work stressors in a sample of 164 managerial and hourly workers employed at 65 different hotels who were each interviewed for eight consecutive days. The two most common stressors were interpersonal tensions at work and overloads (e.g., technology not functioning). The second aim was to determine whether there were differences in the types and frequency of work stressors by job type (i.e., managers v. non-managers), gender, and marital status. Hotel managers reported significantly more stressors than hourly employees. There were no significant differences by gender or marital status. The third aim was to investigate whether the various stressors were linked to hotel employee health and work outcomes. More employee and coworker stressors were linked to more negative physical health symptoms. Also, interpersonal tensions at work were linked to lower job satisfaction and greater turnover intentions. PMID:23794780

  4. miRiadne: a web tool for consistent integration of miRNA nomenclature.

    PubMed

    Bonnal, Raoul J P; Rossi, Riccardo L; Carpi, Donatella; Ranzani, Valeria; Abrignani, Sergio; Pagani, Massimiliano

    2015-07-01

    The miRBase is the official miRNA repository which keeps the annotation updated on newly discovered miRNAs: it is also used as a reference for the design of miRNA profiling platforms. Nomenclature ambiguities generated by loosely updated platforms and design errors lead to incompatibilities among platforms, even from the same vendor. Published miRNA lists are thus generated with different profiling platforms that refer to diverse and not updated annotations. This greatly compromises searches, comparisons and analyses that rely on miRNA names only without taking into account the mature sequences, which is particularly critic when such analyses are carried over automatically. In this paper we introduce miRiadne, a web tool to harmonize miRNA nomenclature, which takes into account the original miRBase versions from 10 up to 21, and annotations of 40 common profiling platforms from nine brands that we manually curated. miRiadne uses the miRNA mature sequence to link miRBase versions and/or platforms to prevent nomenclature ambiguities. miRiadne was designed to simplify and support biologists and bioinformaticians in re-annotating their own miRNA lists and/or data sets. As Ariadne helped Theseus in escaping the mythological maze, miRiadne will help the miRNA researcher in escaping the nomenclature maze. miRiadne is freely accessible from the URL http://www.miriadne.org. PMID:25897123

  5. miRiadne: a web tool for consistent integration of miRNA nomenclature

    PubMed Central

    Bonnal, Raoul J. P.; Rossi, Riccardo L.; Carpi, Donatella; Ranzani, Valeria; Abrignani, Sergio; Pagani, Massimiliano

    2015-01-01

    The miRBase is the official miRNA repository which keeps the annotation updated on newly discovered miRNAs: it is also used as a reference for the design of miRNA profiling platforms. Nomenclature ambiguities generated by loosely updated platforms and design errors lead to incompatibilities among platforms, even from the same vendor. Published miRNA lists are thus generated with different profiling platforms that refer to diverse and not updated annotations. This greatly compromises searches, comparisons and analyses that rely on miRNA names only without taking into account the mature sequences, which is particularly critic when such analyses are carried over automatically. In this paper we introduce miRiadne, a web tool to harmonize miRNA nomenclature, which takes into account the original miRBase versions from 10 up to 21, and annotations of 40 common profiling platforms from nine brands that we manually curated. miRiadne uses the miRNA mature sequence to link miRBase versions and/or platforms to prevent nomenclature ambiguities. miRiadne was designed to simplify and support biologists and bioinformaticians in re-annotating their own miRNA lists and/or data sets. As Ariadne helped Theseus in escaping the mythological maze, miRiadne will help the miRNA researcher in escaping the nomenclature maze. miRiadne is freely accessible from the URL http://www.miriadne.org. PMID:25897123

  6. Use of hygiene protocols to control the spread of viruses in a hotel.

    PubMed

    Sifuentes, Laura Y; Koenig, David W; Phillips, Ronnie L; Reynolds, Kelly A; Gerba, Charles P

    2014-09-01

    The goals of this study were to observe the spread of viruses in a hotel setting and to assess the effectiveness of a hygiene intervention in reducing their spread. Selected fomites in one hotel room were inoculated with bacteriophage ϕx-174, and fomites in a conference center within the same hotel were inoculated using bacteriophage MS2. Cleaning of the contaminated room resulted in the spread of viruses to other rooms by the housekeeping staff. Furthermore, viruses were transferred by hotel guests to the conference center and a communal kitchen area. Additionally, conference attendees transferred viruses from the conference center to their hotel rooms and a communal kitchen area. This study demonstrated how viruses can be spread throughout a hotel setting by both housekeepers and guests. A hygiene intervention, which included providing hand hygiene products and facial tissues to the guests and disinfecting solutions with disposable wipes to the housekeeping staff, was successful in reducing the spread of viruses between the hotel guest rooms and conference center. The hygiene intervention resulted in significantly reduced transfer of the ϕx-174 between the contaminated hotel room and other hotel rooms, communal areas, and the conference center (p = 0.02). PMID:25005587

  7. Serum miRNAs panel (miR-16-2*, miR-195, miR-2861, miR-497) as novel non-invasive biomarkers for detection of cervical cancer

    PubMed Central

    Zhang, Yujuan; Zhang, Donghong; Wang, Fei; Xu, Danfei; Guo, Ye; Cui, Wei

    2015-01-01

    miRNAs have been established as critical layer of regulation during tumorigenesis; extracellular miRNAs are extraordinarily stable; and, quantitative reverse transcript polymerase chain reaction (qRT-PCR) provides a sensitive platform for quantifying miRNAs with a broad dynamic range. Herein, we aimed to establish a serum miRNA signature for diagnosing cervical cancer (CC). In this study, we recruited a cohort of 184 CC, 186 cervical intraepithelial neoplasia (CIN) patients and 193 healthy control subjects. qRT-PCR was performed with serum samples to screen a pool of 444 miRNAs at the initial phase, 66 miRNAs at the training phase, and 7 miRNAs at the validation phase. The profile of 4 circulating miRNAs (miR-16-2*, miR-195, miR-2861, miR-497) was established for CC diagnosis. By Receiver Operating Characteristic (ROC) curve analysis, this 4-miRNA signature showed high accuracy in discriminating CC (AUC = 0.849), and CIN individuals (AUC = 0.734) from healthy controls. Among these 4 miRNAs, only miR-16-2*, but not miR-195, miR-2861 or miR497, shared a similar pattern in sera of breast cancer and ovarian cancer patients. Overall, our studies have identified a novel noninvasive biomarker constituted with a panel of four miRNAs (miR-16-2*, miR-195, miR-2861, miR-497). PMID:26656154

  8. Research on MI in Equipoise

    PubMed Central

    Zuckoff, Allan; Dew, Mary Amanda

    2012-01-01

    Residual ambivalence prior to live organ donation has been shown to predict worse physical and psychological outcomes for the donor following surgery. We are studying whether MI can help individuals who have agreed to become living organ donors to resolve residual ambivalence about their decision. In this situation, ethical practice demands that the counselor take up a stance of equipoise, equally welcoming of strengthened resolve to donate or a decision not to do so. This paper describes our adaptations of MI for this unique application. PMID:23106035

  9. Impact of the Great East Japan Earthquake on Hotel Industry in Pacific Tohoku Prefectures ---From Spatio-Temporal Dependence of Hotel Availability---

    NASA Astrophysics Data System (ADS)

    Sato, A.

    This paper investigates the impact of the Great Japan Earthquake(and subsequent tsunami turmoil) on socio-economic activities by using data on hotel opportunities collected from an electronic hotel booking service. A method to estimate both primary and secondary regional effects of a natural disaster on human behavior is proposed. It is confirmed that temporal variation in the regional share of available hotels before and after a natural disaster may be an indicator to measure the socio-economic impact at each district.

  10. miRNAs in brain development

    SciTech Connect

    Petri, Rebecca; Malmevik, Josephine; Fasching, Liana; Åkerblom, Malin; Jakobsson, Johan

    2014-02-01

    MicroRNAs (miRNAs) are small, non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. In the brain, a large number of miRNAs are expressed and there is a growing body of evidence demonstrating that miRNAs are essential for brain development and neuronal function. Conditional knockout studies of the core components in the miRNA biogenesis pathway, such as Dicer and DGCR8, have demonstrated a crucial role for miRNAs during the development of the central nervous system. Furthermore, mice deleted for specific miRNAs and miRNA-clusters demonstrate diverse functional roles for different miRNAs during the development of different brain structures. miRNAs have been proposed to regulate cellular functions such as differentiation, proliferation and fate-determination of neural progenitors. In this review we summarise the findings from recent studies that highlight the importance of miRNAs in brain development with a focus on the mouse model. We also discuss the technical limitations of current miRNA studies that still limit our understanding of this family of non-coding RNAs and propose the use of novel and refined technologies that are needed in order to fully determine the impact of specific miRNAs in brain development. - Highlights: • miRNAs are essential for brain development and neuronal function. • KO of Dicer is embryonically lethal. • Conditional Dicer KO results in defective proliferation or increased apoptosis. • KO of individual miRNAs or miRNA families is necessary to determine function.

  11. miRegulome: a knowledge-base of miRNA regulomics and analysis

    PubMed Central

    Barh, Debmalya; Kamapantula, Bhanu; Jain, Neha; Nalluri, Joseph; Bhattacharya, Antaripa; Juneja, Lucky; Barve, Neha; Tiwari, Sandeep; Miyoshi, Anderson; Azevedo, Vasco; Blum, Kenneth; Kumar, Anil; Silva, Artur; Ghosh, Preetam

    2015-01-01

    miRNAs regulate post transcriptional gene expression by targeting multiple mRNAs and hence can modulate multiple signalling pathways, biological processes, and patho-physiologies. Therefore, understanding of miRNA regulatory networks is essential in order to modulate the functions of a miRNA. The focus of several existing databases is to provide information on specific aspects of miRNA regulation. However, an integrated resource on the miRNA regulome is currently not available to facilitate the exploration and understanding of miRNA regulomics. miRegulome attempts to bridge this gap. The current version of miRegulome v1.0 provides details on the entire regulatory modules of miRNAs altered in response to chemical treatments and transcription factors, based on validated data manually curated from published literature. Modules of miRegulome (upstream regulators, downstream targets, miRNA regulated pathways, functions, diseases, etc) are hyperlinked to an appropriate external resource and are displayed visually to provide a comprehensive understanding. Four analysis tools are incorporated to identify relationships among different modules based on user specified datasets. miRegulome and its tools are helpful in understanding the biology of miRNAs and will also facilitate the discovery of biomarkers and therapeutics. With added features in upcoming releases, miRegulome will be an essential resource to the scientific community. Availability: http://bnet.egr.vcu.edu/miRegulome. PMID:26243198

  12. The impact of virtual reality functions of a hotel website on travel anxiety.

    PubMed

    Lee, Ook; Oh, Ji-Eun

    2007-08-01

    This study deals with the impact of virtual reality (VR) features that are embedded in a hotel website on travelers' anxiety. Having more information is thought to be a factor in relieving anxiety in travel. A hotel website can be a good place for gathering information about the accommodation. In this study, we posit that a hotel website with VR functions should lead to a reduction in travelers' anxiety about travel. We built a website of a hotel and used VR functions to show the exterior, the lobby, a guest room, and a restaurant through an interactive and spatial shot of the hotel images. The experiment was conducted with a premise that the subjects were about to embark on a journey to an unknown place and to stay at an unknown hotel whose website contained VR functions. The subjects were asked to play with VR functions of the hotel website and then to complete a survey with questions regarding the degree of anxiety on the travel and psychological relief that might have been perceived by the subjects. The result confirms our hypothesis that there is a statistically significant relationship between the degree of travel anxiety and psychological relief caused by the use of VR functions of a hotel website. PMID:17711368

  13. Feasibility Study for Hotel/Motel Career Program for Harper College. Volume XIX, No. 1.

    ERIC Educational Resources Information Center

    Lucas, John A.; And Others

    In spring 1990, a study was conducted at William Rainey Harper College (WRHC) to determine the feasibility of adding a career program in Hotel/Motel Management (HMM) to the current Food Service Program. Surveys were sent to 53 hotels and motels in the WRHC service area to determine employment demands that would affect the hiring of graduates of…

  14. Does Embedding Social Media Channels in Hotel Websites Influence Travelers' Satisfaction and Purchase Intentions?

    ERIC Educational Resources Information Center

    Aluri, Ajay Kumar

    2012-01-01

    In the Internet world today, social media channels have emerged as a top share of Internet usage, and more travelers have started using them to make their hotel plans and purchases. Because of the recommendations of researchers and practitioners, hotel organizations have already embraced social media and have embedded their links on their host…

  15. What Makes Hotel Expatriates Remain in Their Overseas Assignments: A Grounded Theory Study

    ERIC Educational Resources Information Center

    Ho, Zoe Ju-Yu

    2012-01-01

    In this study the researcher uses a qualitative research design to discover what makes hotel expatriates remain in their overseas assignments. In-depth interviews, participant observations, and personal documents are used as data collection methods. Four hotel expatriates are recruited as participants of the study. The collected interview…

  16. Human Resource Development in the Irish Hotel Industry: The Case of the Small Firm.

    ERIC Educational Resources Information Center

    Nolan, Clara

    2002-01-01

    A profile of small businesses in the Irish hotel industry shows that all claim to believe in human resource development but few practice it. Small hotels favor informal, specific job training focused on solution of immediate problems rather than long-term development. (Contains 119 references.) (SK)

  17. Parameters for an Effective Entrepreneurial, Regional, Hotel/Restaurant Management Training Program in Manitoba, Canada.

    ERIC Educational Resources Information Center

    Rossing, Rainer C.

    Owners or managers of 34 small and medium-sized hotels and restaurants in the Assiniboine Community College area were interviewed to acquire information for an entrepreneurial, regional hotel and restaurant (H/R) management training program in Manitoba. A literature review revealed the following: employability, vocational technical, and business…

  18. Hospitality Service: Hotel and Restaurant Management and Culinary Arts Curriculum Guide.

    ERIC Educational Resources Information Center

    Joliet Junior Coll., IL.

    This publication contains competency-based materials for hotel/restaurant management and culinary arts. The materials are designed for students to learn from a work station concept by rotating through a variety of real work settings in a hotel/restaurant environment. In addition, the materials indicate whether or not the students have developed…

  19. Hotel and Restaurant Management; A Bibliography of Books and Audio-Visual Materials.

    ERIC Educational Resources Information Center

    Malkames, James P.; And Others

    This bibliography represents a collection of 1,300 book volumes and audiovisual materials collected by the Luzerne County Community College Library in support of the college's Hotel and Restaurant Management curriculum. It covers such diverse topics as advertising, business practices, decoration, nutrition, hotel law, insurance landscaping, health…

  20. A Trip to the Statler Hilton Hotel. The Special Education Curriculum Series.

    ERIC Educational Resources Information Center

    Kendall, Muriel

    A program designed for high school level work-study classes for students of limited mental ability presents specific curriculum methods and materials to teach information regarding positions available in the hotel industry. A field trip tour of the Boston Statler Hilton Hotel if the focal activity of the unit, and is accompanied by a history of…

  1. The Marketing Effectiveness of Hotel Facebook Pages: From Perspectives of Customers and Messages

    ERIC Educational Resources Information Center

    Leung, Xi Yu

    2012-01-01

    In the hotel industry, social media marketing has become a new trend hoteliers are chasing and an increasing number of hotels are using social media to promote their business. However, the marketing effectiveness of social media is still a big challenge in both academic and business world. Since social media marketing is totally different from…

  2. REEP/Hotel Workplace Literacy Project. Evaluation Report. 1988-1990 Grant Period.

    ERIC Educational Resources Information Center

    Peterson, Morris

    The Arlington Education and Employment Program (REEP)/Hotel Workplace Literacy Project served 230 functionally illiterate adults working in hotels and a real estate maintenance firm in Virginia. Job-related English and math, citizenship preparation, and work awareness instruction was provided. An evaluation found that: (1) the project effectively…

  3. 24 CFR 203.16 - Certificate and contract regarding use of dwelling for transient or hotel purposes.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... use of dwelling for transient or hotel purposes. 203.16 Section 203.16 Housing and Urban Development... use of dwelling for transient or hotel purposes. Every application filed with respect to insurance of... housing or any part thereof covered by the mortgage for transient or hotel purposes, together with...

  4. 24 CFR 203.16 - Certificate and contract regarding use of dwelling for transient or hotel purposes.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... use of dwelling for transient or hotel purposes. 203.16 Section 203.16 Housing and Urban Development... use of dwelling for transient or hotel purposes. Every application filed with respect to insurance of... housing or any part thereof covered by the mortgage for transient or hotel purposes, together with...

  5. 24 CFR 203.16 - Certificate and contract regarding use of dwelling for transient or hotel purposes.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... use of dwelling for transient or hotel purposes. 203.16 Section 203.16 Housing and Urban Development... use of dwelling for transient or hotel purposes. Every application filed with respect to insurance of... housing or any part thereof covered by the mortgage for transient or hotel purposes, together with...

  6. 24 CFR 203.16 - Certificate and contract regarding use of dwelling for transient or hotel purposes.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... use of dwelling for transient or hotel purposes. 203.16 Section 203.16 Housing and Urban Development... use of dwelling for transient or hotel purposes. Every application filed with respect to insurance of... housing or any part thereof covered by the mortgage for transient or hotel purposes, together with...

  7. 24 CFR 203.16 - Certificate and contract regarding use of dwelling for transient or hotel purposes.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... use of dwelling for transient or hotel purposes. 203.16 Section 203.16 Housing and Urban Development... use of dwelling for transient or hotel purposes. Every application filed with respect to insurance of... housing or any part thereof covered by the mortgage for transient or hotel purposes, together with...

  8. miRNA Digger: a comprehensive pipeline for genome-wide novel miRNA mining.

    PubMed

    Yu, Lan; Shao, Chaogang; Ye, Xinghuo; Meng, Yijun; Zhou, Yincong; Chen, Ming

    2016-01-01

    MicroRNAs (miRNAs) are important regulators of gene expression. The recent advances in high-throughput sequencing (HTS) technique have greatly facilitated large-scale detection of the miRNAs. However, thoroughly discovery of novel miRNAs from the available HTS data sets remains a major challenge. In this study, we observed that Dicer-mediated cleavage sites for the processing of the miRNA precursors could be mapped by using degradome sequencing data in both animals and plants. In this regard, a novel tool, miRNA Digger, was developed for systematical discovery of miRNA candidates through genome-wide screening of cleavage signals based on degradome sequencing data. To test its sensitivity and reliability, miRNA Digger was applied to discover miRNAs from four organs of Arabidopsis. The results revealed that a majority of already known mature miRNAs along with their miRNA*s expressed in these four organs were successfully recovered. Notably, a total of 30 novel miRNA-miRNA* pairs that have not been registered in miRBase were discovered by miRNA Digger. After target prediction and degradome sequencing data-based validation, eleven miRNA-target interactions involving six of the novel miRNAs were identified. Taken together, miRNA Digger could be applied for sensitive detection of novel miRNAs and it could be freely downloaded from http://www.bioinfolab.cn/miRNA_Digger/index.html. PMID:26732371

  9. miRNome Analysis of CML Cells.

    PubMed

    Yang, Yadong; Ding, Nan; Dong, Xunong; Fang, Xiangdong

    2016-01-01

    Next-generation sequencing technologies have greatly accelerated the biological and medical progression. As one of the applications, miRNA-Seq is invaluable in detecting and characterizing genome-wide miRNAs of either too high or too low abundance. Besides, it can also be used in detecting novel miRNAs. Here, we describe an ab initio analysis of an example chronic myeloid leukemia miRNA sequencing data set to quantify the global expression of miRNAs, detect differential expression and novel miRNAs, and predict target genes. The run time of this protocol may vary depending on the volume of miRNA sequencing data and available computing resources but takes ~5 h of computing time for typical experiments and less than 1 h of hands-on time. PMID:27581150

  10. Identification of miR-93 as a suitable miR for normalizing miRNA in plasma of tuberculosis patients

    PubMed Central

    Barry, Simone E; Chan, Brian; Ellis, Magda; Yang, YuRong; Plit, Marshall L; Guan, Guangyu; Wang, Xiaolin; Britton, Warwick J; Saunders, Bernadette M

    2015-01-01

    Tuberculosis (TB) remains a major public health issue. New tests to aid diagnoses and monitor the response to therapy are urgently required. There is growing interest in the use of microRNA (miRNA) profiles as diagnostic, prognostic or predictive markers in a range of clinical and infectious diseases, including Mycobacterium tuberculosis infection, however, challenges exist to accurately normalise miRNA levels in cohorts. This study examined the appropriateness of 12 miRs and RNU6B to normalise circulating plasma miRNA levels in individuals with active TB from 2 different geographical and ethnic regions. Twelve miRs (let-7, miR-16, miR-22, miR-26, miR-93, miR-103, miR-191, miR-192, miR-221, miR-423, miR-425 and miR-451) and RNU6B were selected based on their reported production by lung cells, expression in blood and previous use as a reference miRNA. Expression levels were analysed in the plasma of newly diagnosed TB patients from Australia and China compared with individuals with latent TB infection and healthy volunteers. Analysis with both geNorm and NormFinder software identified miR-93 as the most suitable reference miR in both cohorts, either when analysed separately or collectively. Interestingly, there were large variations in the expression levels of some miRs, in particular miR-192 and let-7, between the two cohorts, independent of disease status. These data identify miR-93 is a suitable reference miR for normalizing miRNA levels in TB patients, and highlight how environmental, and possibly ethnic, factors influence miRNA expression levels, demonstrating the necessity of assessing the suitability of reference miRs within the study population. PMID:25753045

  11. Identification of miR-93 as a suitable miR for normalizing miRNA in plasma of tuberculosis patients.

    PubMed

    Barry, Simone E; Chan, Brian; Ellis, Magda; Yang, YuRong; Plit, Marshall L; Guan, Guangyu; Wang, Xiaolin; Britton, Warwick J; Saunders, Bernadette M

    2015-07-01

    Tuberculosis (TB) remains a major public health issue. New tests to aid diagnoses and monitor the response to therapy are urgently required. There is growing interest in the use of microRNA (miRNA) profiles as diagnostic, prognostic or predictive markers in a range of clinical and infectious diseases, including Mycobacterium tuberculosis infection, however, challenges exist to accurately normalise miRNA levels in cohorts. This study examined the appropriateness of 12 miRs and RNU6B to normalise circulating plasma miRNA levels in individuals with active TB from 2 different geographical and ethnic regions. Twelve miRs (let-7, miR-16, miR-22, miR-26, miR-93, miR-103, miR-191, miR-192, miR-221, miR-423, miR-425 and miR-451) and RNU6B were selected based on their reported production by lung cells, expression in blood and previous use as a reference miRNA. Expression levels were analysed in the plasma of newly diagnosed TB patients from Australia and China compared with individuals with latent TB infection and healthy volunteers. Analysis with both geNorm and NormFinder software identified miR-93 as the most suitable reference miR in both cohorts, either when analysed separately or collectively. Interestingly, there were large variations in the expression levels of some miRs, in particular miR-192 and let-7, between the two cohorts, independent of disease status. These data identify miR-93 is a suitable reference miR for normalizing miRNA levels in TB patients, and highlight how environmental, and possibly ethnic, factors influence miRNA expression levels, demonstrating the necessity of assessing the suitability of reference miRs within the study population. PMID:25753045

  12. Hotel Cedes 7 months' savings for total lighting retrofit

    SciTech Connect

    Warrock, A.M.

    1983-07-11

    In an unusual shared-savings agreement, the Hilton Florida Center at Orlando, where a retrofit program was begun two years ago, will give up all savings from a lighting retrofit program for seven months, avoiding upfront costs of equipment purchase, and will then become sole owner of the equipment and beneficiary of the savings. The four-month-old program has improved the lighting and cut electricity costs $2000 to $2500 per month, which would have been a six-month payback. Contracts for two other hotels are expected where retrofitting has begun. Retrofit details are given.

  13. Solar cooling system performance, Frenchman's Reef Hotel, Virgin Islands

    NASA Technical Reports Server (NTRS)

    Harber, H.

    1981-01-01

    The operational and thermal performance of a variety of solar systems are described. The Solar Cooling System was installed in a hotel at St. Thomas, U. S. Virgin Islands. The system consists of the evacuated glass tube collectors, two 2500 gallon tanks, pumps, computerized controller, a large solar optimized industrial sized lithium bromide absorption chiller, and associated plumbing. Solar heated water is pumped through the system to the designed public areas such as lobby, lounges, restaurant and hallways. Auxiliary heat is provided by steam and a heat exchanger to supplement the solar heat.

  14. Solar cooling system performance, Frenchman's Reef Hotel, Virgin Islands

    NASA Astrophysics Data System (ADS)

    Harber, H.

    1981-09-01

    The operational and thermal performance of a variety of solar systems are described. The Solar Cooling System was installed in a hotel at St. Thomas, U. S. Virgin Islands. The system consists of the evacuated glass tube collectors, two 2500 gallon tanks, pumps, computerized controller, a large solar optimized industrial sized lithium bromide absorption chiller, and associated plumbing. Solar heated water is pumped through the system to the designed public areas such as lobby, lounges, restaurant and hallways. Auxiliary heat is provided by steam and a heat exchanger to supplement the solar heat.

  15. Association of miR-34a, miR-130a, miR-150 and miR-155 polymorphisms with the risk of ischemic stroke.

    PubMed

    Choi, Gun Ho; Ko, Ki Han; Kim, Jung Oh; Kim, Jinkwon; Oh, Seung Hun; Han, In Bo; Cho, Kyung Gi; Kim, Ok Joon; Bae, Jinkun; Kim, Nam Keun

    2016-07-01

    MicroRNAs (miRNAs or miRs) are small (19-23 nt) non-coding RNA molecules that are endogenous regulators of gene expression. Previous studies have found that some miRNAs are related to the progression of ischemia in the cerebral artery. Furthermore, a recent study found a significant association between miRNA single nucleotide polymorphisms (SNPs) and the risk of ischemic stroke. Therefore, it may be valuable to investigate associations between megakaryocyte formation-related miRNA polymorphisms and the prevalence of ischemic stroke. We thus conducted a case-control study of 1,000 individuals who were screened for 4 miRNA polymorphisms (miR‑34a rs6577555C>A, miR-130a rs731384C>T, miR-150 rs73056059G>A and miR‑155 rs767649T>A) by PCR-RFLP analysis. The study population comprised 596 patients with ischemic stroke and 404 control subjects without any history of neurological disorders. We observed associations between miRNA polymorphisms and individual stroke subtypes. The miR‑150 polymorphisms were significantly associated with ischemic stroke subgroups, such as left anterior descending artery (LAD) disease [GG vs. AA: adjusted odds ratio (AOR), 1.922; 95% confidence interval (CI), 1.003-3.681] and cardioembolism (GG vs. AA: AOR, 2.996; 95% CI, 1.293-6.939). Additionally, Cox proportional analysis indicated that the miR‑150GA genotype was associated with survival in patients with ischemic stroke [adjusted hazard ratio (HR), 2.063; 95% CI, 1.142-3.727; P=0.017] and with the LAD subgroup [adjusted HR, 3.021; 95% CI, 1.345-6.785; P=0.008]. Our findings suggest that miR‑150 polymorphisms may contribute to the development of ischemic stroke and may potentially act as biomarkers to predict the risk of ischemic stroke. To the best of our knowledge, this is the first study to evaluate the association between miRNA polymorphisms (miR-34aC>A, miR-130aC>T, miR-150G>A and miR-155T>A) and ischemic stroke. PMID:27246008

  16. What do hotels and hospitals have in common? How we can learn from the hotel industry to take better care of patients

    PubMed Central

    Zygourakis, Corinna C.; Rolston, John D.; Treadway, James; Chang, Susan; Kliot, Michel

    2014-01-01

    Despite widely divergent public perceptions and goals, hotels and hospitals share many core characteristics. Both serve demanding and increasingly well-informed clienteles, both employ a large hierarchy of workers with varying levels of responsibility, and both have payments that are increasingly tied to customer/patient evaluations. In the hotel industry, decades of management experience and market research have led to widespread improvements and innovations that improve customer satisfaction. But there has been incredibly little cross-fertilization between the hotel and hospital industries. In this paper, we first consider the changes in the healthcare system that are forcing hospitals to become more concerned with patient satisfaction. We discuss the similarities and differences between the hotel and hospital industries, and then outline several of the unique challenges that neurosurgeons face in taking care of patients and increasing their comfort. We cite specific lessons from the hotel industry that can be applied to patients’ preadmission, check-in, hospital stay, discharge planning, and poststay experiences. We believe that hospitals can and should leverage the successful advances within the hotel industry to improve patient satisfaction, without having to repeat identical research or market experimentation. We hope this will lead to rapid improvements in patient experiences and overall wellbeing. PMID:24818061

  17. What do hotels and hospitals have in common? How we can learn from the hotel industry to take better care of patients.

    PubMed

    Zygourakis, Corinna C; Rolston, John D; Treadway, James; Chang, Susan; Kliot, Michel

    2014-01-01

    Despite widely divergent public perceptions and goals, hotels and hospitals share many core characteristics. Both serve demanding and increasingly well-informed clienteles, both employ a large hierarchy of workers with varying levels of responsibility, and both have payments that are increasingly tied to customer/patient evaluations. In the hotel industry, decades of management experience and market research have led to widespread improvements and innovations that improve customer satisfaction. But there has been incredibly little cross-fertilization between the hotel and hospital industries. In this paper, we first consider the changes in the healthcare system that are forcing hospitals to become more concerned with patient satisfaction. We discuss the similarities and differences between the hotel and hospital industries, and then outline several of the unique challenges that neurosurgeons face in taking care of patients and increasing their comfort. We cite specific lessons from the hotel industry that can be applied to patients' preadmission, check-in, hospital stay, discharge planning, and poststay experiences. We believe that hospitals can and should leverage the successful advances within the hotel industry to improve patient satisfaction, without having to repeat identical research or market experimentation. We hope this will lead to rapid improvements in patient experiences and overall wellbeing. PMID:24818061

  18. So, Why Sol-Mi? American Music Education

    ERIC Educational Resources Information Center

    Bennett, Peggy D.

    2005-01-01

    Walk into any primary grade music class in the U.S., and you will likely hear teacher and students singing a musical greeting, such as "Good morning boys and girls" (sol-mi-mi-sol-sol-mi) and the response "Good morning Miss Purdy" (sol-mi-mi-sol-mi-mi). Since about the 1970s, teachers have been beginning and ending music class for young children…

  19. miRNA-based drought regulation in wheat.

    PubMed

    Akdogan, Guray; Tufekci, Ebru Derelli; Uranbey, Serkan; Unver, Turgay

    2016-05-01

    MicroRNAs (miRNAs) are a class of small non-coding regulatory RNAs that regulate gene expression by guiding target mRNA cleavage or translational inhibition. Drought is a common environmental stress influencing crop growth and development. To date, it has been reported that a number of plant miRNA are involved in drought stress response. In this study, we comparatively investigated drought stress-responsive miRNAs in the root and leaf of bread wheat (Triticum aestivum cv. Sivas 111/33) by miRNA microarray screening. miRNA microarray analysis showed that 285 miRNAs (207 upregulated and 78 downregulated) and 244 miRNAs (115 upregulated and 129 downregulated) were differentially expressed in leaf and root tissues, respectively. Among the differentially expressed miRNAs, 23 miRNAs were only expressed in the leaf and 26 miRNAs were only expressed in the root of wheat growth under drought stress. Upon drought treatment, expression of miR159, miR160, miR166, miR169, miR172, miR395, miR396, miR408, miR472, miR477, miR482, miR1858, miR2118, and miR5049 were found to be significantly differentiated in bread wheat. The regulatory network analysis showed that miR395 has connections with a number of target transcripts, and miR159 and miR319 share a number of target genes. Drought-tolerant and drought-sensitive wheat cultivars showed altered expression pattern upon drought stress in terms of investigated miRNA and their target transcript expression level. PMID:26141043

  20. miRNA and Vascular Cell Movement

    PubMed Central

    Yue, Junming

    2011-01-01

    miRNAs are a new class of endogenous small RNAs that negatively regulate gene expression at the posttranscriptional level. Accumulating experimental evidence shows that miRNAs regulate cellular apoptosis, proliferation, differentiation, and migration. Dysregulation of miRNA expression leads to various human diseases including cancer and cardiovascular disease. miRNA maturation is regulated at multiple steps by different mechanisms, including miRNA editing, hairpin loop binding, self-regulation, and cross-talk with other signaling pathways. Vascular cell movement plays a pivotal role in the development of various cancers and cardiovascular diseases. miRNAs have been found to regulate vascular cell movement. Presently the chemically synthesized antagomir, miRNA mimics have been widely used in investigating the biological functions of miRNA genes. The viral vectors, including adenoviral, lentiviral, and adeno-associated viral vectors, have been used to efficiently overexpress or knockdown miRNAs in vitro and in vivo. Therefore, targeting vascular cell movement using miRNA-based drug or gene therapy would provide a novel therapeutic approach in the treatment of cancers and vascular diseases. PMID:21241758

  1. miR-340 suppresses glioblastoma multiforme.

    PubMed

    Huang, Daquan; Qiu, Shuwei; Ge, Ruiguang; He, Lei; Li, Mei; Li, Yi; Peng, Ying

    2015-04-20

    Deregulation of microRNAs (miRs) contributes to tumorigenesis. Down-regulation of miR-340 is observed in multiple types of cancers. However, the biological function of miR-340 in glioblastoma multiforme (GBM) remains largely unknown. In the present study, we demonstrated that expression of miR-340 was downregulated in both glioma cell lines and tissues. Survival of GBM patients with high levels of miR-340 was significantly extended in comparison to patients expressing low miR-340 levels. Biological functional experiments showed that the restoration of miR-340 dramatically inhibited glioma cell proliferation, induced cell-cycle arrest and apoptosis, suppressed cell motility and promoted autophagy and terminal differentiation. Mechanistic studies disclosed that, miR-340 over-expression suppressed several oncogenes including p-AKT, EZH2, EGFR, BMI1 and XIAP. Furthermore, ROCK1 was validated as a direct functional target miR-340 and silencing of ROCK1 phenocopied the anti-tumor effect of mR-340. Our findings indicate an important role of miR-340 as a glioma killer, and suggest a potential prognosis biomarker and therapeutic target for GBM. PMID:25831237

  2. Best practices to reduce the accident rate hotel

    NASA Astrophysics Data System (ADS)

    García Revilla, M. R.; Kahale Carrillo, D. T.

    2014-10-01

    Examining the available databases and existing tourism organizations can conclude that appear studies on accidents and their relationship with other variables. But in our case we want to assess this relationship in the performance of the hotel in relation to lower the accident rate. The Industrial Safety studies analyzing this accident causes (why they happen), their sources (committed activities), their agents (participants work means), its type (how the events occur or develop), all in order to develop prevention. In our case, as accidents happen because people commit wrongful acts or because the equipment, tools, machinery or workplaces are not in proper conditions, the preventive point of view we analyze through the incidence of workplace accidents hotel subsector. The crash occurs because there is a risk, so that adequate control of it would avoid despite individual factors. Absenteeism or absence from work was taken into account first by Dubois in 1977, as he realized the time lost in the nineteenth century due to the long working hours, which included the holidays. Motivation and job satisfaction were the elements that have been most important in the phenomenon of social psychology.

  3. Comparison of non-prewhitening and Hotelling observer models

    NASA Astrophysics Data System (ADS)

    Burgess, Arthur E.

    1995-04-01

    Recent investigations of human signal detection performance for noise limited tasks have used statistically defined signal or image parameters. The Bayesian ideal observer procedure is then nonlinear and analysis becomes mathematically intractable. Linear, but suboptimal, observer models have been proposed for mathematical convenience. Experiments by Rolland and Barrett involving detection of completely defined signals in white noise superimposed on statistically defined (Lumpy) backgrounds showed that the Fisher-Hotelling model gave a good fit while the simple nonprewhitening (NPW) matched filter gave a poor fit. Burgess showed that the NPW model can be modified to fit their data by adding a spatial frequency filter with response similar to the human contrast sensitivity function. New experimental results will be presented demonstrating that neither model is satisfactory. The results of our experiments done with a variety of spectral densities for the background can be described by a Fisher-Hotelling model modified to include simple circularly symmetric spatial frequency channels as proposed by Myers and Barrett. However, results of our variable viewing distance experiments do not agree with predictions of this simple channelized model. It will be necessary to use a more complex F model with physiologically reasonable spatial frequency channels.

  4. Human annoyance and reactions to hotel room specific noises

    NASA Astrophysics Data System (ADS)

    Everhard, Ian L.

    2001-05-01

    A new formula is presented where multiple annoyance sources and transmission loss values of any partition are combined to produce a new single number rating of annoyance. The explanation of the formula is based on theoretical psychoacoustics and survey testing used to create variables used to weight the results. An imaginary hotel room is processed through the new formula and is rated based on theoretical survey results that would be taken by guests of the hotel. The new single number rating compares the multiple sources of annoyance to a single imaginary unbiased source where absolute level is the only factor in stimulating a linear rise in annoyance [Fidell et al., J. Acoust. Soc. Am. 66, 1427 (1979); D. M. Jones and D. E. Broadbent, ``Human performance and noise,'' in Handbook of Noise Control, 3rd ed., edited by C. M. Harris (ASA, New York, 1998), Chap. 24; J. P. Conroy and J. S. Roland, ``STC Field Testing and Results,'' in Sound and Vibration Magazine, Acoustical Publications, pp. 10-15 (July 2003)].

  5. miRTarBase 2016: updates to the experimentally validated miRNA-target interactions database

    PubMed Central

    Chou, Chih-Hung; Chang, Nai-Wen; Shrestha, Sirjana; Hsu, Sheng-Da; Lin, Yu-Ling; Lee, Wei-Hsiang; Yang, Chi-Dung; Hong, Hsiao-Chin; Wei, Ting-Yen; Tu, Siang-Jyun; Tsai, Tzi-Ren; Ho, Shu-Yi; Jian, Ting-Yan; Wu, Hsin-Yi; Chen, Pin-Rong; Lin, Nai-Chieh; Huang, Hsin-Tzu; Yang, Tzu-Ling; Pai, Chung-Yuan; Tai, Chun-San; Chen, Wen-Liang; Huang, Chia-Yen; Liu, Chun-Chi; Weng, Shun-Long; Liao, Kuang-Wen; Hsu, Wen-Lian; Huang, Hsien-Da

    2016-01-01

    MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides, which negatively regulate the gene expression at the post-transcriptional level. This study describes an update of the miRTarBase (http://miRTarBase.mbc.nctu.edu.tw/) that provides information about experimentally validated miRNA-target interactions (MTIs). The latest update of the miRTarBase expanded it to identify systematically Argonaute-miRNA-RNA interactions from 138 crosslinking and immunoprecipitation sequencing (CLIP-seq) data sets that were generated by 21 independent studies. The database contains 4966 articles, 7439 strongly validated MTIs (using reporter assays or western blots) and 348 007 MTIs from CLIP-seq. The number of MTIs in the miRTarBase has increased around 7-fold since the 2014 miRTarBase update. The miRNA and gene expression profiles from The Cancer Genome Atlas (TCGA) are integrated to provide an effective overview of this exponential growth in the miRNA experimental data. These improvements make the miRTarBase one of the more comprehensively annotated, experimentally validated miRNA-target interactions databases and motivate additional miRNA research efforts. PMID:26590260

  6. Risk miRNA screening of ovarian cancer based on miRNA functional synergistic network

    PubMed Central

    2014-01-01

    Background miRNAs are proved to have causal roles in tumorgenesis involving various types of human cancers, but the mechanism is not clear. We aimed to explore the effect of miRNAs on the development of ovarian cancer and the underlying mechanism. Methods The miRNA expression profile GSE31801 was downloaded from GEO (Gene Expression Omnibus) database. Firstly, the differentially expressed miRNAs were screened. Target genes of the miRNAs were collected from TargetScan, PicTar, miRanda, and DIANA-microT database, then the miRNA-miRNA co-regulating network was constructed using miRNA pairs with common regulated target genes. Next, the functional modules in the network were studied, the miRNA pairs regulated at least one modules were enriched to form the miRNA functional synergistic network (MFSN). Results Risk miRNA were selected in MFSN according to the topological structure. Transcript factors (TFs) in MFSN were identified, followed by the miRNA-transcript factor networks construction. Totally, 42 up- and 61 down-regulated differentially expressed miRNAs were identified, of which 68 formed 2292 miRNA pairs in the miRNA-miRNA co-regulating network. GO: 0007268 (synaptic transmission) and GO: 0019226 (transmission of nerve impulse) were the two common functions of miRNAs in MFSN, and hsa-miR-579 (36), hsa-miR-942 (31), hsa-miR-105 (31), hsa-miR-150 (34), and hsa-miR-27a* (32) were selected as the hub nodes in MFSN. Conclusions In all, 17 TFs, including CREM, ERG, and CREB1 were screened as the cancer related TFs in MFSN. Other TFs, such as BIN1, FOXN3, FOXK1, FOXP2, and ESRRG with high degrees may be inhibited in ovarian cancer. MFSN gave us a new shed light on the mechanism studies in ovarian cancer. PMID:24444095

  7. MicroRNAs, miR-154, miR-299-5p, miR-376a, miR-376c, miR-377, miR-381, miR-487b, miR-485-3p, miR-495 and miR-654-3p, mapped to the 14q32.31 locus, regulate proliferation, apoptosis, migration and invasion in metastatic prostate cancer cells.

    PubMed

    Formosa, A; Markert, E K; Lena, A M; Italiano, D; Finazzi-Agro', E; Levine, A J; Bernardini, S; Garabadgiu, A V; Melino, G; Candi, E

    2014-10-30

    miRNAs act as oncogenes or tumor suppressors in a wide variety of human cancers, including prostate cancer (PCa). We found a severe and consistent downregulation of miRNAs, miR-154, miR-299-5p, miR-376a, miR-376c, miR-377, miR-381, miR-487b, miR-485-3p, miR-495 and miR-654-3p, mapped to the 14q32.31 region in metastatic cell lines as compared with normal prostatic epithelial cells (PrEC). In specimens of human prostate (28 normals, 99 primary tumors and 13 metastases), lower miRNA levels correlated significantly with a higher incidence of metastatic events and higher prostate specific antigen (PSA) levels, with similar trends observed for lymph node invasion and the Gleason score. We transiently transfected 10 members of the 14q32.31 cluster in normal prostatic epithelial cell lines and characterized their affect on malignant cell behaviors, including proliferation, apoptosis, migration and invasion. Finally, we identified FZD4, a gene important for epithelial-to-mesenchymal transition in (PCa), as a target of miR-377. PMID:24166498

  8. miRClassify: an advanced web server for miRNA family classification and annotation.

    PubMed

    Zou, Quan; Mao, Yaozong; Hu, Lingling; Wu, Yunfeng; Ji, Zhiliang

    2014-02-01

    MicroRNA (miRNA) family is a group of miRNAs that derive from the common ancestor. Normally, members from the same miRNA family have similar physiological functions; however, they are not always conserved in primary sequence or secondary structure. Proper family prediction from primary sequence will be helpful for accurate identification and further functional annotation of novel miRNA. Therefore, we introduced a novel machine learning-based web server, the miRClassify, which can rapidly identify miRNA from the primary sequence and classify it into a miRNA family regardless of similarity in sequence and structure. Additionally, the medical implication of the miRNA family is also provided when it is available in PubMed. The web server is accessible at the link http://datamining.xmu.edu.cn/software/MIR/home.html. PMID:24480175

  9. Computational and in vitro Investigation of miRNA-Gene Regulations in Retinoblastoma Pathogenesis: miRNA Mimics Strategy

    PubMed Central

    Venkatesan, Nalini; Deepa, Perinkulam Ravi; Khetan, Vikas; Krishnakumar, Subramanian

    2015-01-01

    PURPOSE Retinoblastoma (RB), a primary pediatric intraocular tumor, arises from primitive retinal layers. Several novel molecular strategies are being developed for the clinical management of RB. miRNAs are known to regulate cancer-relevant biological processes. Here, the role of selected miRNAs, namely, miR-532-5p and miR-486-3p, has been analyzed for potential therapeutic targeting in RB. METHODS A comprehensive bioinformatic analysis was performed to predict the posttranscriptional regulators (miRNAs) of the select panel of genes [Group 1: oncogenes (HMGA2, MYCN, SYK, FASN); Group 2: cancer stem cell markers (TACSTD, ABCG2, CD133, CD44, CD24) and Group 3: cell cycle regulatory proteins (p53, MDM2)] using Microcosm, DIANALAB, miRBase v 18, and REFSEQ database, and RNA hybrid. The expressions of five miRNAs, namely, miR-146b-5p, miR-532-5p, miR-142-5p, miR-328, and miR-486-3p, were analyzed by qRT–PCR on primary RB tumor samples (n = 30; including 17 invasive RB tumors and 13 noninvasive RB tumors). Detailed complementary alignment between 5’ seed sequence of differentially expressed miRNAs and the sequence of target genes was determined. Based on minimum energy level and piCTAR scores, the gene targets were selected. Functional roles of these miRNA clusters were studied by using mimics in cultured RB (Y79, Weri Rb-1) cells in vitro. The gene targets (SYK and FASN) of the studied miRNAs were confirmed by qRT-PCR and western blot analysis. Cell proliferation and apoptotic studies were performed. RESULTS Nearly 1948 miRNAs were identified in the in silico analysis, From this list, only 9 upregulated miRNAs (miR-146b-5p, miR-305, miR-663b, miR-299, miR-532-5p, miR-892b, miR-501, miR-142-5p, and miR-513b) and 10 downregulated miRNAs (miR-1254, miR-328, miR-133a, miR-1287, miR-1299, miR-375, miR-486-3p, miR-720, miR-98, and miR-122*) were found to be common with the RB serum miRNA profile. Downregulation of five miRNAs (miR-146b-5p, miR-532-5p, miR-142-5p, miR-328

  10. miRNAs in human cancer

    PubMed Central

    Farazi, Thalia A.; Spitzer, Jessica I.; Morozov, Pavel; Tuschl, Thomas

    2010-01-01

    Mature microRNAs (miRNAs) are single-stranded RNA molecules of 20- to 23-nucleotide (nt) length that control gene expression in many cellular processes. These molecules typically reduce the stability of mRNAs, including those of genes that mediate processes in tumorigenesis, such as inflammation, cell cycle regulation, stress response, differentiation, apoptosis, and invasion. miRNA targeting is mostly achieved through specific base-pairing interactions between the 5′ end (“seed” region) of the miRNA and sites within coding and untranslated regions (UTRs) of mRNAs; target sites in the 3′ UTR lead to more effective mRNA destabilization. Since miRNAs frequently target hundreds of mRNAs, miRNA regulatory pathways are complex. To provide a critical overview of miRNA dysregulation in cancer we first discuss the methods currently available for studying the role of miRNAs in cancer and then review miRNA genomic organization, biogenesis, and mechanism of target recognition examining how these processes are altered in tumorigenesis. Given the critical role miRNAs play in tumorigenesis processes and their disease specific expression, they hold potential as therapeutic targets and novel biomarkers. PMID:21125669

  11. miRNA sensitivity to Drosha levels correlates with pre-miRNA secondary structure

    PubMed Central

    Sperber, Henrik; Beem, Alan; Shannon, Sandra; Jones, Ross; Banik, Pratyusha; Chen, Yu; Ku, Sherman; Varani, Gabriele; Yao, Shuyuan; Ruohola-Baker, Hannele

    2014-01-01

    microRNAs (miRNAs) are crucial for cellular development and homeostasis. In order to better understand regulation of miRNA biosynthesis, we studied cleavage of primary miRNAs by Drosha. While Drosha knockdown triggers an expected decrease of many mature miRNAs in human embryonic stem cells (hESC), a subset of miRNAs are not reduced. Statistical analysis of miRNA secondary structure and fold change of expression in response to Drosha knockdown showed that absence of mismatches in the central region of the hairpin, 5 and 9–12 nt from the Drosha cutting site conferred decreased sensitivity to Drosha knockdown. This suggests that, when limiting, Drosha processes miRNAs without mismatches more efficiently than mismatched miRNAs. This is important because Drosha expression changes over cellular development and the fold change of expression for miRNAs with mismatches in the central region correlates with Drosha levels. To examine the biochemical relationship directly, we overexpressed structural variants of miRNA-145, miRNA-137, miRNA-9, and miRNA-200b in HeLa cells with and without Drosha knockdown; for these miRNAs, elimination of mismatches in the central region increased, and addition of mismatches decreased their expression in an in vitro assay and in cells with low Drosha expression. Change in Drosha expression can be a biologically relevant mechanism by which eukaryotic cells control miRNA profiles. This phenomenon may explain the impact of point mutations outside the seed region of certain miRNAs. PMID:24677349

  12. miRNA polymorphisms (miR‑146a, miR‑149, miR‑196a2 and miR‑499) are associated with the risk of coronary artery disease.

    PubMed

    Sung, Jung-Hoon; Kim, Sang-Hoon; Yang, Woo-In; Kim, Won-Jang; Moon, Jae-Youn; Kim, In Jai; Cha, Dong-Hun; Cho, Seung-Yun; Kim, Jung Oh; Kim, Kyeong Ah; Kim, Ok-Joon; Lim, Sang-Wook; Kim, Nam-Keun

    2016-09-01

    Small non‑coding microRNAs (miRNAs) are not only important for heart and vascular development but are also important in cardiovascular pathophysiology and diseases, such as ischemia and atherosclerosis‑related diseases. However, the effect of miR‑146a, miR‑149, miR‑196a2 and miR‑499 polymorphisms on coronary artery disease (CAD) susceptibility remain unknown. The aim of the present study was to examine the genotype frequencies of miR‑146a, miR‑149, miR‑196a2 and miR‑499 polymorphisms in patients with CAD, and assess their clinical applications for diagnosing and monitoring CAD. Using polymerase chain reaction‑amplified DNA, microRNA polymorphisms were analyzed in 522 patients with CAD and 535 control subjects. The miR‑149 rs2292832 C>T and miR‑196a2 rs11614913 T>C polymorphisms were shown to be significantly associated with CAD prevalence. In subgroup analyses according to disease severity, the miR‑146a rs2910164GG genotype was significantly associated with CAD risk in the stent ≥2 group. In addition, miR‑146aG/‑149T/‑196a2C/‑499 G allele combination was significantly associated with CAD prevalence (G‑T‑C‑G and G‑C‑C‑G of miR‑146a/‑149/‑196a2/‑499). The combination genotypes of miR‑146aGG/149TC+CC and miR‑149CC/196a2TC were significantly associated with CAD incidence. In subgroup analyses, miR‑146a rs2910164 C>G increased the risk of developing CAD in non‑smoking, hypertensive and nondiabetic subgroups. Furthermore, miR‑149 rs2292832 C>T and miR‑196a2 rs11614913 T>C was shown to increase CAD risk in females and patients aged >63 years old. The miR‑149T allele, miR‑196a2C allele and miR‑146aG/‑149T/‑196a2C/‑499 G allele combination were associated with CAD pathogenesis. The combined effects of environmental factor and genotype combination of miRNA polymorphisms may contribute to CAD prevalence. PMID:27430349

  13. A fuzzy MCDM model with objective and subjective weights for evaluating service quality in hotel industries

    NASA Astrophysics Data System (ADS)

    Zoraghi, Nima; Amiri, Maghsoud; Talebi, Golnaz; Zowghi, Mahdi

    2013-12-01

    This paper presents a fuzzy multi-criteria decision-making (FMCDM) model by integrating both subjective and objective weights for ranking and evaluating the service quality in hotels. The objective method selects weights of criteria through mathematical calculation, while the subjective method uses judgments of decision makers. In this paper, we use a combination of weights obtained by both approaches in evaluating service quality in hotel industries. A real case study that considered ranking five hotels is illustrated. Examples are shown to indicate capabilities of the proposed method.

  14. Factors Influencing e-Business Adoption in the Greek Hotel Sector

    NASA Astrophysics Data System (ADS)

    Samanta, Ir.; Kyriazopoulos, P.

    The purpose of this research is to identify the impact of business process improvement in the area of e-marketing in the hotel industry. The research identifies the barriers which block organizational change effort. A sample of thirty hotels in the city of Athens was used.This paper presents a SWOT analysis of the hotel sector, identifying the strengths, weaknesses, opportunities and threats that firms faced in the process of change. The results show that the majority of firms use, to a small extent, the e-marketing concept to improve their communication strategy and reach market segments.

  15. How Clean Are Hotel Rooms? Part I: Visual Observations vs. Microbiological Contamination.

    PubMed

    Almanza, Barbara A; Kirsch, Katie; Kline, Sheryl Fried; Sirsat, Sujata; Stroia, Olivia; Choi, Jin Kyung; Neal, Jay

    2015-01-01

    Current evidence of hotel room cleanliness is based on observation rather than empirically based microbial assessment. The purpose of the study described here was to determine if observation provides an accurate indicator of cleanliness. Results demonstrated that visual assessment did not accurately predict microbial contamination. Although testing standards have not yet been established for hotel rooms and will be evaluated in Part II of the authors' study, potential microbial hazards included the sponge and mop (housekeeping cart), toilet, bathroom floor, bathroom sink, and light switch. Hotel managers should increase cleaning in key areas to reduce guest exposure to harmful bacteria. PMID:26427262

  16. Early Bird Visions and Telchnology for Space Hotel

    NASA Astrophysics Data System (ADS)

    Amekrane, R.; Holze, C.

    2002-01-01

    The International Space Station was planed for research purposes. In 2001 the first private man, Denis Tito, visited the ISS and the second private man, Mark Shuttleworth is following him. Space pioneers as Wernher von Braun and Sir Arthur C. Clarke had the dream that one day a space station in earth orbit will host tourists. It is evident that the ISS is not designed to host tourists. Therefore the dream is still open. Asking the question "how should a space station should look like to host tourists?" the German Aerospace Society DGLR e.V. initiated in April 2001 a contest under the patronage of . Mr. Joerg Feustel-Buechl, the Director of Manned Spaceflight and Microgravity, European Space Agency (ESA). Because the definition and design of living space is the content of architecture the approach was to gather new ideas from young architects in cooperation with space experts. This contest was directed at students of architecture and the task set was to design a hotel for the earth orbit and to accommodate 220 guests. The contest got the name "Early Bird - Visions of a Space Hotel". The results and models of the student's work were shown in an exhibition in Hamburg/Germany, which was open to the public from September 19th till October 20th 2001. During the summer term 2001 fifty students from the university occupied themselves with the topic, "Design of a hotel for space". During this time seventeen designs were completed. Having specialists, as volunteers, in the field of space in charge meant that it could be ensured that the designs reflected a certain possibility of being able to be realized. Within this interdisciplinary project both parties learned from each other. The 17 different designs were focused on the expectations and needs of a future space tourist. The design are for sure not feasible today, but the designs are in that sense realistic that they could be built in future. This paper will present an overview of the 17 designs as a vision of a future

  17. NORTH EMBANKMENT IN FOREGROUND, WITH (LR) SUBSTATION (MI98D), POWERHOUSE (MI98C), ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    NORTH EMBANKMENT IN FOREGROUND, WITH (L-R) SUBSTATION (MI-98-D), POWERHOUSE (MI-98-C), AND COOKE DAM POND IN BACKGROUND. VIEW TO SOUTH - Cooke Hydroelectric Plant, North Embankment, Cook Dam Road at Au Sable River, Oscoda, Iosco County, MI

  18. Non-inhibited miRNAs shape the cellular response to anti-miR

    PubMed Central

    Androsavich, John R.; Chau, B. Nelson

    2014-01-01

    Identification of primary microRNA (miRNA) gene targets is critical for developing miRNA-based therapeutics and understanding their mechanisms of action. However, disentangling primary target derepression induced by miRNA inhibition from secondary effects on the transcriptome remains a technical challenge. Here, we utilized RNA immunoprecipitation (RIP) combined with competitive binding assays to identify novel primary targets of miR-122. These transcripts physically dissociate from AGO2-miRNA complexes when anti-miR is spiked into liver lysates. mRNA target displacement strongly correlated with expression changes in these genes following in vivo anti-miR dosing, suggesting that derepression of these targets directly reflects changes in AGO2 target occupancy. Importantly, using a metric based on weighted miRNA expression, we found that the most responsive mRNA target candidates in both RIP competition assays and expression profiling experiments were those with fewer alternative seed sites for highly expressed non-inhibited miRNAs. These data strongly suggest that miRNA co-regulation modulates the transcriptomic response to anti-miR. We demonstrate the practical utility of this ‘miR-target impact’ model, and encourage its incorporation, together with the RIP competition assay, into existing target prediction and validation pipelines. PMID:24810853

  19. miRTex: A Text Mining System for miRNA-Gene Relation Extraction

    PubMed Central

    Li, Gang; Ross, Karen E.; Arighi, Cecilia N.; Peng, Yifan; Wu, Cathy H.; Vijay-Shanker, K.

    2015-01-01

    MicroRNAs (miRNAs) regulate a wide range of cellular and developmental processes through gene expression suppression or mRNA degradation. Experimentally validated miRNA gene targets are often reported in the literature. In this paper, we describe miRTex, a text mining system that extracts miRNA-target relations, as well as miRNA-gene and gene-miRNA regulation relations. The system achieves good precision and recall when evaluated on a literature corpus of 150 abstracts with F-scores close to 0.90 on the three different types of relations. We conducted full-scale text mining using miRTex to process all the Medline abstracts and all the full-length articles in the PubMed Central Open Access Subset. The results for all the Medline abstracts are stored in a database for interactive query and file download via the website at http://proteininformationresource.org/mirtex. Using miRTex, we identified genes potentially regulated by miRNAs in Triple Negative Breast Cancer, as well as miRNA-gene relations that, in conjunction with kinase-substrate relations, regulate the response to abiotic stress in Arabidopsis thaliana. These two use cases demonstrate the usefulness of miRTex text mining in the analysis of miRNA-regulated biological processes. PMID:26407127

  20. Evaluation of inhibition of miRNA expression induced by anti-miRNA oligonucleotides.

    PubMed

    Chae, Dong-Kyu; Ban, Eunmi; Yoo, Young Sook; Baik, Ja-Hyun; Song, Eun Joo

    2016-07-01

    MicroRNAs (miRNAs) are short RNA molecules that control the expression of mRNAs associated with various biological processes. Therefore, deregulated miRNAs play an important role in the pathogenesis of diseases. Numerous studies aimed at developing novel miRNA-based drugs or determining miRNA functions have been conducted by inhibiting miRNAs using anti-miRNA oligonucleotides (AMOs), which inhibit the function by hybridizing with miRNA. To increase the binding affinity and specificity to target miRNA, AMOs with various chemical modifications have been developed. Evaluating the potency of these various types of AMOs is an essential step in their development. In this study, we developed a capillary electrophoresis with laser-induced fluorescence (CE-LIF) method to evaluate the potency of AMOs by measuring changes in miRNA levels with fluorescence-labeled ssDNA probes using AMO-miR-23a, which inhibits miR-23a related to lung cancer. In order to eliminate interference by excess AMOs during hybridization of the ssDNA probe with the miR-23a, the concentration of the ssDNA probe was optimized. This newly developed method was used to compare the potency of two different modified AMOs. The data were supported by the results of a luciferase assay. This study demonstrated that CE-LIF analysis could be used to accurately evaluate AMO potency in biological samples. PMID:27178549

  1. Spatiotemporal plasticity of miRNAs functions: The miR-17-92 case.

    PubMed

    Bonaldi, Tiziana; Mihailovich, Marija

    2016-05-01

    The functional effect of a specific miRNA is tightly linked to the transcriptome, thus having the potential to elicit distinct outcomes in different cellular states. Our recent discovery of a dual role of the miR-17-92 cluster, which shifts from oncogene to tumor suppressor during lymphoma progression, exemplifies the spatiotemporal plasticity of miRNAs. PMID:27314099

  2. Michigan Molecular Interactions (MiMI): putting the jigsaw puzzle together

    PubMed Central

    Jayapandian, Magesh; Chapman, Adriane; Tarcea, V. Glenn; Yu, Cong; Elkiss, Aaron; Ianni, Angela; Liu, Bin; Nandi, Arnab; Santos, Carlos; Andrews, Philip; Athey, Brian; States, David; Jagadish, H. V.

    2007-01-01

    Protein interaction data exists in a number of repositories. Each repository has its own data format, molecule identifier and supplementary information. Michigan Molecular Interactions (MiMI) assists scientists searching through this overwhelming amount of protein interaction data. MiMI gathers data from well-known protein interaction databases and deep-merges the information. Utilizing an identity function, molecules that may have different identifiers but represent the same real-world object are merged. Thus, MiMI allows the users to retrieve information from many different databases at once, highlighting complementary and contradictory information. To help scientists judge the usefulness of a piece of data, MiMI tracks the provenance of all data. Finally, a simple yet powerful user interface aids users in their queries, and frees them from the onerous task of knowing the data format or learning a query language. MiMI allows scientists to query all data, whether corroborative or contradictory, and specify which sources to utilize. MiMI is part of the National Center for Integrative Biomedical Informatics (NCIBI) and is publicly available at: . PMID:17130145

  3. Shared-savings cuts hotel's losses from EMS removal

    SciTech Connect

    Galvin, C.

    1982-11-08

    A shared-savings contract will minimize the Myrtle Beach, SC Downtown Holiday Inn's losses of replacing a poorly performing Energy Master energy-management system with Scientific Atlanta equipment. The contract with Energy Master Inc. (EMI), which saved Holiday Inn the $80,000 to $90,000 purchase price, also permitted removal (a year after installation) of the equipment when it failed to generate energy savings. A dispute between Associated Energy Consultants (AEC), which was to receive half the savings in exchange for arranging the equipment financing, is described. At $51,745, the 262-point Scientific Atlanta system should have a 1.7-year payback. The hotel's electric bills were $2000 a month lower during the first three months of operation. (DCK)

  4. 90. Paso Del Norte Hotel, 115117 South El Paso St.,east ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    90. Paso Del Norte Hotel, 115-117 South El Paso St.,east facade, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  5. S. EL PASO ST., 8018031/2 (HOTEL ORIZABA), EAST ELEVATION, WEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    S. EL PASO ST., 801-8031/2 (HOTEL ORIZABA), EAST ELEVATION, WEST SIDE OF STREET - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  6. S. EL PASO ST., 8018031/2 (HOTEL ORIZABA), NORTH AND EAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    S. EL PASO ST., 801-8031/2 (HOTEL ORIZABA), NORTH AND EAST FACADES, WEST SIDE OF STREET - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  7. 60. South El Paso St., 801803 1/2 (Hotel Orizaba), east ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    60. South El Paso St., 801-803 1/2 (Hotel Orizaba), east elevation, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

  8. miR-isomiRExp: a web-server for the analysis of expression of miRNA at the miRNA/isomiR levels.

    PubMed

    Guo, Li; Yu, Jiafeng; Liang, Tingming; Zou, Quan

    2016-01-01

    MicroRNA (miRNA) locus has been found that can generate a series of varied isomiR sequences. Most studies always focus on determining miRNA level, however, the canonical miRNA sequence is only a specific member in the multiple isomiRs. Some studies have shown that isomiR sequences play versatile roles in biological progress, and the analysis and research should be simultaneously performed at the miRNA/isomiR levels. Based on the biological characteristics of miRNA and isomiR, we developed miR-isomiRExp to analyze expression pattern of miRNA at the miRNA/isomiR levels, provide insights into tracking miRNA/isomiR maturation and processing mechanisms, and reveal functional characteristics of miRNA/isomiR. Simultaneously, we also performed expression analysis of specific human diseases using public small RNA sequencing datasets based on the analysis platform, which may help in surveying the potential deregulated miRNA/isomiR expression profiles, especially sequence and function-related isomiRs for further interaction analysis and study. The miR-isomiRExp platform provides miRNA/isomiR expression patterns and more information to study deregulated miRNA loci and detailed isomiR sequences. This comprehensive analysis will enrich experimental miRNA studies. miR-isomiRExp is available at http://mirisomirexp.aliapp.com. PMID:27009551

  9. miR-isomiRExp: a web-server for the analysis of expression of miRNA at the miRNA/isomiR levels

    PubMed Central

    Guo, Li; Yu, Jiafeng; Liang, Tingming; Zou, Quan

    2016-01-01

    MicroRNA (miRNA) locus has been found that can generate a series of varied isomiR sequences. Most studies always focus on determining miRNA level, however, the canonical miRNA sequence is only a specific member in the multiple isomiRs. Some studies have shown that isomiR sequences play versatile roles in biological progress, and the analysis and research should be simultaneously performed at the miRNA/isomiR levels. Based on the biological characteristics of miRNA and isomiR, we developed miR-isomiRExp to analyze expression pattern of miRNA at the miRNA/isomiR levels, provide insights into tracking miRNA/isomiR maturation and processing mechanisms, and reveal functional characteristics of miRNA/isomiR. Simultaneously, we also performed expression analysis of specific human diseases using public small RNA sequencing datasets based on the analysis platform, which may help in surveying the potential deregulated miRNA/isomiR expression profiles, especially sequence and function-related isomiRs for further interaction analysis and study. The miR-isomiRExp platform provides miRNA/isomiR expression patterns and more information to study deregulated miRNA loci and detailed isomiR sequences. This comprehensive analysis will enrich experimental miRNA studies. miR-isomiRExp is available at http://mirisomirexp.aliapp.com. PMID:27009551

  10. miRISC recruits decapping factors to miRNA targets to enhance their degradation

    PubMed Central

    Nishihara, Tadashi; Zekri, Latifa; Braun, Joerg E.; Izaurralde, Elisa

    2013-01-01

    MicroRNA (miRNA)-induced silencing complexes (miRISCs) repress translation and promote degradation of miRNA targets. Target degradation occurs through the 5′-to-3′ messenger RNA (mRNA) decay pathway, wherein, after shortening of the mRNA poly(A) tail, the removal of the 5′ cap structure by decapping triggers irreversible decay of the mRNA body. Here, we demonstrate that miRISC enhances the association of the decapping activators DCP1, Me31B and HPat with deadenylated miRNA targets that accumulate when decapping is blocked. DCP1 and Me31B recruitment by miRISC occurs before the completion of deadenylation. Remarkably, miRISC recruits DCP1, Me31B and HPat to engineered miRNA targets transcribed by RNA polymerase III, which lack a cap structure, a protein-coding region and a poly(A) tail. Furthermore, miRISC can trigger decapping and the subsequent degradation of mRNA targets independently of ongoing deadenylation. Thus, miRISC increases the local concentration of the decapping machinery on miRNA targets to facilitate decapping and irreversibly shut down their translation. PMID:23863838

  11. Search and selection hotel system in Surabaya based on geographic information system (GIS) with fuzzy logic

    NASA Astrophysics Data System (ADS)

    Purbandini, Taufik

    2016-03-01

    Surabaya is a metropolitan city in Indonesia. When the visitor has an interest in Surabaya for several days, then the visitor was looking for lodging that is closest to the interests of making it more efficient and practical. It was not a waste of time for the businessman because of congestion and so we need full information about the hotel as an inn during a stay in Surabaya began name, address of the hotel, the hotel's website, the distance from the hotel to the destination until the display of the map along the route with the help of Google Maps. This system was designed using fuzzy logic which aims to assist the user in making decisions. Design of hotel search and selection system was done through four stages. The first phase was the collection of data and as the factors that influence the decision-making along with the limit values of these factors. Factors that influence covers a distance of the hotel, the price of hotel rooms, and hotel reviews. The second stage was the processing of data and information by creating membership functions. The third stage was the analysis of systems with fuzzy logic. The steps were performed in systems analysis, namely fuzzification, inference using Mamdani, and defuzzification. The last stage was the design and construction of the system. Designing the system using use case diagrams and activity diagram to describe any process that occurs. Development system includes system implementation and evaluation systems. Implementation of mobile with Android-based system so that these applications were user friendly.

  12. miRNAFold: a web server for fast miRNA precursor prediction in genomes

    PubMed Central

    Tav, Christophe; Tempel, Sébastien; Poligny, Laurent; Tahi, Fariza

    2016-01-01

    Computational methods are required for prediction of non-coding RNAs (ncRNAs), which are involved in many biological processes, especially at post-transcriptional level. Among these ncRNAs, miRNAs have been largely studied and biologists need efficient and fast tools for their identification. In particular, ab initio methods are usually required when predicting novel miRNAs. Here we present a web server dedicated for miRNA precursors identification at a large scale in genomes. It is based on an algorithm called miRNAFold that allows predicting miRNA hairpin structures quickly with high sensitivity. miRNAFold is implemented as a web server with an intuitive and user-friendly interface, as well as a standalone version. The web server is freely available at: http://EvryRNA.ibisc.univ-evry.fr/miRNAFold. PMID:27242364

  13. miRNAFold: a web server for fast miRNA precursor prediction in genomes.

    PubMed

    Tav, Christophe; Tempel, Sébastien; Poligny, Laurent; Tahi, Fariza

    2016-07-01

    Computational methods are required for prediction of non-coding RNAs (ncRNAs), which are involved in many biological processes, especially at post-transcriptional level. Among these ncRNAs, miRNAs have been largely studied and biologists need efficient and fast tools for their identification. In particular, ab initio methods are usually required when predicting novel miRNAs. Here we present a web server dedicated for miRNA precursors identification at a large scale in genomes. It is based on an algorithm called miRNAFold that allows predicting miRNA hairpin structures quickly with high sensitivity. miRNAFold is implemented as a web server with an intuitive and user-friendly interface, as well as a standalone version. The web server is freely available at: http://EvryRNA.ibisc.univ-evry.fr/miRNAFold. PMID:27242364

  14. Fungal DNA in hotel rooms in Europe and Asia--associations with latitude, precipitation, building data, room characteristics and hotel ranking.

    PubMed

    Norbäck, Dan; Cai, Gui-Hong

    2011-10-01

    There is little information on the indoor environment in hotels. Analysis of fungal DNA by quantitative PCR (qPCR) is a new method which can detect general and specific sequences. Dust was collected through swab sampling of door frames in 69 hotel rooms in 20 countries in Europe and Asia (2007-2009). Five sequences were detected by qPCR: total fungal DNA, Aspergillus and Penicillium DNA (Asp/Pen DNA), Aspergillus versicolor (A. versicolor DNA), Stachybotrys chartarum (S. chartarum DNA) and Streptomyces spp. (Streptomyces DNA). Associations were analysed by multiple linear regression. Total fungal DNA (GM = 1.08 × 10(8) cell equivalents m(-2); GSD = 6.36) and Asp/Pen DNA (GM = 1.79 × 10(7) cell equivalents m(-2); GSD = 10.12) were detected in all rooms. A. versicolor DNA, S. chartarum DNA and Streptomyces DNA were detected in 84%, 28% and 47% of the samples. In total, 20% of the rooms had observed dampness/mould, and 30% had odour. Low latitude (range 1.5-64.2 degrees) was a predictor of Asp/Pen DNA. Seaside location, lack of mechanical ventilation, and dampness or mould were other predictors of total fungal DNA and Asp/Pen DNA. Hotel ranking (Trip Advisor) or self-rated quality of the interior of the hotel room was a predictor of total fungal DNA, A. versicolor DNA and Streptomyces DNA. Odour was a predictor of S. chartarum DNA. In conclusion, fungal DNA in swab samples from hotel rooms was related to latitude, seaside location, ventilation, visible dampness and indoor mould growth. Hotels in tropical areas may have 10-100 times higher levels of common moulds such as Aspergillus and Penicillium species, as compared to a temperate climate zone. PMID:21897974

  15. A framework for stochastic simulation of distribution practices for hotel reservations

    SciTech Connect

    Halkos, George E.; Tsilika, Kyriaki D.

    2015-03-10

    The focus of this study is primarily on the Greek hotel industry. The objective is to design and develop a framework for stochastic simulation of reservation requests, reservation arrivals, cancellations and hotel occupancy with a planning horizon of a tourist season. In Greek hospitality industry there have been two competing policies for reservation planning process up to 2003: reservations coming directly from customers and a reservations management relying on tour operator(s). Recently the Internet along with other emerging technologies has offered the potential to disrupt enduring distribution arrangements. The focus of the study is on the choice of distribution intermediaries. We present an empirical model for the hotel reservation planning process that makes use of a symbolic simulation, Monte Carlo method, as, requests for reservations, cancellations, and arrival rates are all sources of uncertainty. We consider as a case study the problem of determining the optimal booking strategy for a medium size hotel in Skiathos Island, Greece. Probability distributions and parameters estimation result from the historical data available and by following suggestions made in the relevant literature. The results of this study may assist hotel managers define distribution strategies for hotel rooms and evaluate the performance of the reservations management system.

  16. "There's no-fresh air there": narratives of smoke exposure among residents of extended-stay hotels.

    PubMed

    Lewinson, Terri; Bryant, Lawrence Oliver

    2015-05-01

    Hotel environments have been identified as places where hospitality workers and patrons are at an increased risk for smoke exposure and associated health hazards. However, little research has been conducted to understand experiences of long-term hotel residents. This article presents narrative accounts of tobacco smoke exposure from 37 residents at extended-stay hotels in a large metropolitan Atlanta county. Residents' narratives included an awareness of smoking as an unhealthy habit but a shared activity that facilitates social engagement at the hotel. Secondhand smoke narratives included descriptions of exposure from roommates and hotel neighbors. Thirdhand smoke narratives included reports of persistent pollution and smoke residue in the hotel environment. These results suggest a need for further research to understand the extent and impact of tobacco smoke exposure among this understudied population. The implications of this research support the efforts of social workers to engage in clean air advocacy and policy making for a vulnerable population. PMID:26027415

  17. Serum miR-206 and miR-132 as Potential Circulating Biomarkers for Mild Cognitive Impairment.

    PubMed

    Xie, Bing; Zhou, Huimin; Zhang, Rui; Song, Mei; Yu, Lulu; Wang, Lan; Liu, Zanchao; Zhang, Qingfu; Cui, Dongsheng; Wang, Xueyi; Xu, Shunjiang

    2015-01-01

    MicroRNAs (miRNAs), a class of small, non-coding RNA molecules with gene regulatory functions, have emerged to play a critical role in the pathogenesis of a variety of diseases. Recently, circulating miRNAs have been reported as potential biomarkers for various pathologic conditions. The present study was performed to investigate the potential role of circulating miRNAs as diagnostic biomarkers for mild cognitive impairment (MCI). We collected 66 patients with MCI and 76 normal controls from our previous cross-sectional cohort study. Seven miRNAs (miR-206, miR-132, miR-193b, miR-130b, miR-20a, miR-296, and miR-329) related to Alzheimer's disease (AD) were detected in serum using a quantitative real-time PCR (qRT-PCR) method. Each miRNA's diagnostic performance was evaluated by receiver operating characteristic curves and the areas under curves (AUC) analysis. The levels of miR-206 and miR-132 in MCI patients' serum were significantly elevated compared to normal controls. Combining detection of miR-206 and miR-132 achieved the highest AUC of 0.981, followed by test of miR-206 (AUC = 0.880) and miR-132 (AUC = 0.912) separately. Importantly, miR-206 and miR-132 were respectively correlated with the Montreal Cognitive Assessment score in MCI patients. These results preliminarily indicated that circulating miR-206 and miR-132 as novel miRNAs upregulated in MCI patient were potential biomarkers for diagnosis of MCI. PMID:25589731

  18. Expression Change of miR-214 and miR-135 during Muscle Differentiation

    PubMed Central

    Honardoost, Maryam; Soleimani, Masoud; Arefian, Ehsan; Sarookhani, Mohammad reza

    2015-01-01

    Objective MicroRNAs (miRNAs) are a class of small non-coding RNAs that play pivotal roles in many biological processes such as regulating skeletal muscle development where alterations in miRNA expression are reported during myogenesis. In this study, we aimed to investigate the impact of predicted miRNAs and their target genes on the myoblast to myocyte differentiation process. Materials and Methods This experimental study was conducted on the C2C12 cell line. Using a bioinformatics approach, miR-214 and miR-135 were selected according to their targets as potential factors in myoblast to myocyte differentiation induced by 3% horse serum. Immunocytochemistry (ICC) was undertaken to confirm the differentiation process and quantitative real-time polymerase chain reaction (PCR) to determine the expression level of miRNAs and their targets. Results During myoblast to myocyte differentiation, miR-214 was significantly down- regulated while miRNA-135, Irs2, Akt2 and Insr were overexpressed during the process. Conclusion miR-214 and miR-135 are potential regulators of myogenesis and are involved in skeletal muscle development through regulating the IRS/PI3K pathway. PMID:26464817

  19. miR-145 and miR-143 Regulate Smooth Muscle Cell Fate Decisions

    PubMed Central

    Cordes, Kimberly R.; Sheehy, Neil T.; White, Mark; Berry, Emily; Morton, Sarah U.; Muth, Alecia N.; Lee, Ting-Hein; Miano, Joseph M.; Ivey, Kathryn N.; Srivastava, Deepak

    2009-01-01

    SUMMARY microRNAs are regulators of myriad cellular events, but evidence for a single microRNA that can efficiently differentiate multipotent cells into a specific lineage or regulate direct reprogramming of cells into an alternate cell fate has been elusive. Here, we show that miR-145 and miR-143 are co-transcribed in multipotent cardiac progenitors before becoming localized to smooth muscle cells, including neural crest stem cell–derived vascular smooth muscle cells. miR-145 and miR-143 were direct transcriptional targets of serum response factor, myocardin and Nkx2.5, and were downregulated in injured or atherosclerotic vessels containing proliferating, less differentiated smooth muscle cells. miR-145 was necessary for myocardin-induced reprogramming of adult fibroblasts into smooth muscle cells and sufficient to induce differentiation of multipotent neural crest stem cells into vascular smooth muscle. Furthermore, miR-145 and miR-143 cooperatively targeted a network of transcription factors, including Klf4, myocardin, and Elk-1 to promote differentiation and repress proliferation of smooth muscle cells. These findings demonstrate that miR-145 can direct the smooth muscle fate and that miR-145 and miR-143 function to regulate the quiescent versus proliferative phenotype of smooth muscle cells. PMID:19578358

  20. MiR-221 and miR-130a Regulate Lung Airway and Vascular Development

    PubMed Central

    Mujahid, Sana

    2013-01-01

    Epithelial-mesenchymal interactions play a crucial role in branching morphogenesis, but very little is known about how endothelial cells contribute to this process. Here, we examined how anti-angiogenic miR-221 and pro-angiogenic miR-130a affect airway and vascular development in the fetal lungs. Lung-specific effects of miR-130a and miR-221 were studied in mouse E14 whole lungs cultured for 48 hours with anti-miRs or mimics to miR-130a and miR-221. Anti-miR 221 treated lungs had more distal branch generations with increased Hoxb5 and VEGFR2 around airways. Conversely, mimic 221 treated lungs had reduced airway branching, dilated airway tips and decreased Hoxb5 and VEGFR2 in mesenchyme. Anti-miR 130a treatment led to reduced airway branching with increased Hoxa5 and decreased VEGFR2 in the mesenchyme. Conversely, mimic 130a treated lungs had numerous finely arborized branches extending into central lung regions with diffusely localized Hoxa5 and increased VEGFR2 in the mesenchyme. Vascular morphology was analyzed by GSL-B4 (endothelial cell-specific lectin) immunofluorescence. Observed changes in airway morphology following miR-221 inhibition and miR-130a enhancement were mirrored by changes in vascular plexus formation around the terminal airways. Mouse fetal lung endothelial cells (MFLM-91U) were used to study microvascular cell behavior. Mimic 221 treatment resulted in reduced tube formation and cell migration, where as the reverse was observed with mimic 130a treatment. From these data, we conclude that miR-221 and miR-130a have opposing effects on airway and vascular morphogenesis of the developing lung. PMID:23409087

  1. Circulating extracellular miR-22, miR-155, and miR-365 as candidate biomarkers to assess transport-related stress in turkeys.

    PubMed

    Lecchi, C; Marques, A T; Redegalli, M; Meani, S; Vinco, L J; Bronzo, V; Ceciliani, F

    2016-07-01

    MicroRNA (miRNA) have been identified in circulating blood and might have the potential to be used as biomarkers for several pathophysiological conditions. To identify miRNA that are altered following stress events, turkeys (Meleagris gallopavo) were subjected to 2 h of road transportation. The expression levels of five circulating miRNA, namely miR-22, miR-155-5p, miR-181a-3p, miR-204 and miR-365-3p, were detected and assessed by quantitative polymerase chain reaction using TaqMan® probes, as potential biomarkers of stress. The areas under the receiver operating characteristic curves were then used to evaluate the diagnostic performance of miRNA. A panel of three stress-responsive miRNA, miR-22, miR-155 and miR-365 were identified; their expression levels were significantly higher after road transportation and the area under the curve (AUC) were 0.763, 0.71 and 0.704, respectively. Combining the three miRNA a specificity similar to the one found for the three miRNA separately was found. The AUC of the weighted average of the three miRNA was 0.763. This preliminary study suggests that the expression levels of circulating miR-22, miR-155 and miR-365 are increased during transport-related stress and that they may have diagnostic value to discriminate between stressed- and unstressed animals. PMID:26760121

  2. Influence of interstitial Mn on magnetism in the room-temperature ferromagnet <mi>Mn>1+<mi>δSb>

    SciTech Connect

    Taylor, Alice E.; Berlijn, Tom; Hahn, Steven E.; May, Andrew F.; Williams, Travis J.; Poudel, Lekhanath N; Calder, Stuart A.; Fishman, Randy Scott; Stone, Matthew B.; Aczel, Adam A.; Cao, Huibo; Lumsden, Mark D.; Christianson, Andrew D.

    2015-06-15

    We report elastic and inelastic neutron scattering measurements of the high-TC ferromagnet <mi>Mn>1+<mi>δSb>. Measurements were performed on a large, TC = 434 K, single crystal with interstitial Mn content of δ ≈ 0.13. The neutron diffraction results reveal that the interstitial Mn has a magnetic moment, and that it is aligned antiparallel to the main Mn moment. We perform density functional theory calculations including the interstitial Mn, and find the interstitial to be magnetic in agreement with the diffraction data. The inelastic neutron scattering measurements reveal two features in the magnetic dynamics: i) a spin-wave-like dispersion emanating from ferromagnetic Bragg positions (H K 2n), and ii) a broad, non-dispersive signal centered at forbidden Bragg positions (H K 2n+1). The inelastic spectrum cannot be modeled by simple linear spin-wave theory calculations, and appears to be significantly altered by the presence of the interstitial Mn ions. Finally, the results show that the influence of the interstitial Mn on the magnetic state in this system is more important than previously understood.

  3. MI high power operation and future plans

    SciTech Connect

    Kourbanis, Ioanis; /Fermilab

    2008-09-01

    Fermilab's Main Injector on acceleration cycles to 120 GeV has been running a mixed mode operation delivering beam to both the antiproton source for pbar production and to the NuMI[1] target for neutrino production since 2005. On January 2008 the slip stacking process used to increase the beam to the pbar target was expanded to include the beam to the NuMI target increasing both the beam intensity and power. The current high power MI operation will be described along with the near future plans.

  4. miR-200a/miR-141 and miR-205 upregulation might be associated with hormone receptor status and prognosis in endometrial carcinomas

    PubMed Central

    Dong, Ying; Si, Jing-Wen; Li, Wen-Ting; Liang, Li; Zhao, Jian; Zhou, Mei; Li, Dong; Li, Ting

    2015-01-01

    The aim of this study was to compare the clinicopathological significance of miR-200a/miR-141 and miR-205 expression in endometrioid carcinomas (ECs) versus nonendometrioid carcinomas (NECs) and to assess their correlation with hormone receptor status. miR-200a/miR-141 and miR-205 expression in 154 endometrial cancers was determined by qRT-PCR. The status of estrogen and progesterone receptor (ER/PR) was assessed using immunohistochemistry. miR-200a/miR-141 and miR-205 increased significantly in ECs and in NECs. The expression level of miR-200a was significantly higher in NECs than in ECs (P = 0.025). Furthermore, there was a trend that NECs with worse clinicopathological variables had a higher miR-200a expression, while an inverse trend existed in ECs. miR-205 upregulation occurred frequently in NECs without lymph node metastases (P = 0.030), whereas such association was not present in ECs. Interestingly, In ECs, miR-200a/miR-141 upregulation occurred frequently in the hormone receptor positive subgroups than the negative subgroups (P < 0.05). Similarly, the expression level of miR-205 was higher in the hormone receptor positive subgroups and the association between miR-205 and PR reached statistical significance (P = 0.024). In contrast, in NECs, a negative correlation was found between miR-200a/miR-141 and ER or PR status. Meanwhile, in ECs, miR-200a upregulation correlated with prolonged survival in the ER positive subgroup (P = 0.046), whereas an inverse trend existed in the ER negative subgroup. Our findings suggest that miR-200a/miR-141 and miR-205 increased significantly in ECs and in NECs. However, they might behave differently in ECs versus NECs. miR-200a/miR-141 and miR-205 might be associated with hormone receptor status in endometrial cancer and may possess prognostic impacts. PMID:26045795

  5. A Runx2/miR-3960/miR-2861 regulatory feedback loop during mouse osteoblast differentiation.

    PubMed

    Hu, Rong; Liu, Wei; Li, Hui; Yang, Li; Chen, Chao; Xia, Zhu-Ying; Guo, Li-Juan; Xie, Hui; Zhou, Hou-De; Wu, Xian-Ping; Luo, Xiang-Hang

    2011-04-01

    Our recent study showed that miR-2861 promotes osteoblast differentiation by targeting histone deacetylase 5, resulting in increased runt-related transcription factor 2 (Runx2) protein production. Here we identified another new microRNA (miRNA) (miR-3960) that played a regulatory role in osteoblast differentiation through a regulatory feedback loop with miR-2861. miR-3960 and miR-2861 were found clustered at the same loci. miR-3960 was transcribed during bone morphogenic protein 2 (BMP2)-induced osteogenesis of ST2 stromal cells. Overexpression of miR-3960 promoted BMP2-induced osteoblastogenesis. However, the inhibition of miR-3960 expression attenuated the osteoblastogenesis. Homeobox A2 (Hoxa2), a repressor of Runx2 expression, was confirmed to be a target of miR-3960. Electrophoretic mobility shift assay and chromatin immunoprecipitation experiments confirmed that Runx2 bound to the promoter of the miR-3960/miR-2861 cluster. Furthermore, overexpression of Runx2 induced miR-3960/miR-2861 transcription, and block of Runx2 expression attenuated BMP2-induced miR-3960/miR-2861 transcription. Here we report that miR-3960 and miR-2861, transcribed together from the same miRNA polycistron, both function in osteoblast differentiation through a novel Runx2/miR-3960/miR-2861 regulatory feedback loop. Our findings provide new insights into the roles of miRNAs in osteoblast differentiation. PMID:21324897

  6. Obtaining miRNA-Target Interaction Information from miRWalk2.0.

    PubMed

    Parveen, Alisha; Gretz, Norbert; Dweep, Harsh

    2016-01-01

    miRWalk2.0 (http://zmf.umm.uni-heidelberg.de/mirwalk2) is a freely accessible, regularly updated comprehensive archive supplying the largest available collection of predicted and experimentally verified miRNA-target interactions, with various novel and unique features to assist the scientific community. Approximately 949 million interactions between 11,748 miRNAs, 308,700 genes, and 68,460 lncRNAs are documented in miRWalk2.0 with 5,146,217 different kinds of identifiers to offer a one-stop site to collect an abundance of information. This article describes a schematic workflow on how to obtain miRNA-target interactions from miRWalk2.0. © 2016 by John Wiley & Sons, Inc. PMID:27603021

  7. miRNome in myocardial infarction: Future directions and perspective

    PubMed Central

    Boštjančič, Emanuela; Glavač, Damjan

    2014-01-01

    MicroRNAs (miRNAs), which are small and non-coding RNAs, are genome encoded from viruses to humans. They contribute to various developmental, physiological and pathological processes in living organisms. A huge amount of research results revealed that miRNAs regulate these processes also in the heart. miRNAs may have cell-type-specific or tissue-specific expression patterns or may be expressed ubiquitously. Primary studies of miRNA involvement in hypertrophy, heart failure and myocardial infarction analyzed miRNAs that are enriched in or specific for cardiomyocytes; however, growing evidence suggest that other miRNAs, not cardiac or muscle-specific, play a significant role in cardiovascular disease. Abnormal miRNA regulation has been shown to be involved in cardiac diseases, suggesting that miRNAs might affect cardiac structure and function. In this review, we focus on miRNAs that have been found to contribute to the pathogenesis of myocardial infarction (MI) and the response post-MI and characterized as diagnostic, prognostic and therapeutic targets. The majority of these studies were performed using mouse and rat models of MI, with a focus on the identification of basic cellular and molecular pathways involved in MI and in the response post-MI. Much research has also been performed on animal and human plasma samples from MI individuals to identify miRNAs that are possible prognostic and/or diagnostic targets of MI and other MI-related diseases. A large proportion of research is focused on miRNAs as promising therapeutic targets and biomarkers of drug responses and/or stem cell treatment approaches. However, only a few studies have described miRNA expression in human heart tissue following MI. PMID:25276296

  8. MiRduplexSVM: A High-Performing MiRNA-Duplex Prediction and Evaluation Methodology

    PubMed Central

    Karathanasis, Nestoras; Tsamardinos, Ioannis; Poirazi, Panayiota

    2015-01-01

    We address the problem of predicting the position of a miRNA duplex on a microRNA hairpin via the development and application of a novel SVM-based methodology. Our method combines a unique problem representation and an unbiased optimization protocol to learn from mirBase19.0 an accurate predictive model, termed MiRduplexSVM. This is the first model that provides precise information about all four ends of the miRNA duplex. We show that (a) our method outperforms four state-of-the-art tools, namely MaturePred, MiRPara, MatureBayes, MiRdup as well as a Simple Geometric Locator when applied on the same training datasets employed for each tool and evaluated on a common blind test set. (b) In all comparisons, MiRduplexSVM shows superior performance, achieving up to a 60% increase in prediction accuracy for mammalian hairpins and can generalize very well on plant hairpins, without any special optimization. (c) The tool has a number of important applications such as the ability to accurately predict the miRNA or the miRNA*, given the opposite strand of a duplex. Its performance on this task is superior to the 2nts overhang rule commonly used in computational studies and similar to that of a comparative genomic approach, without the need for prior knowledge or the complexity of performing multiple alignments. Finally, it is able to evaluate novel, potential miRNAs found either computationally or experimentally. In relation with recent confidence evaluation methods used in miRBase, MiRduplexSVM was successful in identifying high confidence potential miRNAs. PMID:25961860

  9. Foodborne gastroenteritis due to Norwalk virus in a Winnipeg hotel.

    PubMed Central

    Sekla, L; Stackiw, W; Dzogan, S; Sargeant, D

    1989-01-01

    Within 1 week four separate incidents of gastroenteritis presumed to be foodborne were reported by guests of a Winnipeg hotel. Investigation revealed poor food-handling practices and illness among the kitchen staff. Elevated bacterial counts and Escherichia coli were found in 15 of 24 samples of food tested, and Staphylococcus aureus was isolated from 2 pastry samples. Culture of 14 stool samples for bacteria yielded Clostridium perfringens in 1 sample from a staff member and coagulase-positive S. aureus in 2 samples from staff members and 3 from guests. All of the S. aureus isolates were nonenterotoxigenic and had three different phage patterns. Electron microscopy and immunoelectron microscopy revealed the prototype Norwalk virus in five (56%) of nine stool samples; four samples were from guests, and one was from a kitchen employee. The employee had had diarrhea 24 hours before the first outbreak and was thus believed to be the source of the virus infection, possibly through food handling. This is the first report of Norwalk virus isolation and the first of foodborne Norwalk virus transmission in Canada. A review of foodborne Norwalk virus infections is presented. Images Fig. 1 Fig. 2 PMID:2541881

  10. Foodborne gastroenteritis due to Norwalk virus in a Winnipeg hotel.

    PubMed

    Sekla, L; Stackiw, W; Dzogan, S; Sargeant, D

    1989-06-15

    Within 1 week four separate incidents of gastroenteritis presumed to be foodborne were reported by guests of a Winnipeg hotel. Investigation revealed poor food-handling practices and illness among the kitchen staff. Elevated bacterial counts and Escherichia coli were found in 15 of 24 samples of food tested, and Staphylococcus aureus was isolated from 2 pastry samples. Culture of 14 stool samples for bacteria yielded Clostridium perfringens in 1 sample from a staff member and coagulase-positive S. aureus in 2 samples from staff members and 3 from guests. All of the S. aureus isolates were nonenterotoxigenic and had three different phage patterns. Electron microscopy and immunoelectron microscopy revealed the prototype Norwalk virus in five (56%) of nine stool samples; four samples were from guests, and one was from a kitchen employee. The employee had had diarrhea 24 hours before the first outbreak and was thus believed to be the source of the virus infection, possibly through food handling. This is the first report of Norwalk virus isolation and the first of foodborne Norwalk virus transmission in Canada. A review of foodborne Norwalk virus infections is presented. PMID:2541881

  11. Mi-DISCOVERER: A bioinformatics tool for the detection of mi-RNA in human genome.

    PubMed

    Arshad, Saadia; Mumtaz, Asia; Ahmad, Freed; Liaquat, Sadia; Nadeem, Shahid; Mehboob, Shahid; Afzal, Muhammad

    2010-01-01

    MicroRNAs (miRNAs) are 22 nucleotides non-coding RNAs that play pivotal regulatory roles in diverse organisms including the humans and are difficult to be identified due to lack of either sequence features or robust algorithms to efficiently identify. Therefore, we made a tool that is Mi-Discoverer for the detection of miRNAs in human genome. The tools used for the development of software are Microsoft Office Access 2003, the JDK version 1.6.0, BioJava version 1.0, and the NetBeans IDE version 6.0. All already made miRNAs softwares were web based; so the advantage of our project was to make a desktop facility to the user for sequence alignment search with already identified miRNAs of human genome present in the database. The user can also insert and update the newly discovered human miRNA in the database. Mi-Discoverer, a bioinformatics tool successfully identifies human miRNAs based on multiple sequence alignment searches. It's a non redundant database containing a large collection of publicly available human miRNAs. PMID:21364831

  12. The NuMI Neutrino Beam

    DOE PAGESBeta

    Adamson, P.; Anderson, K.; Andrews, M.; Andrews, R.; Anghel, I.; Augustine, D.; Aurisano, A.; Avvakumov, S.; Ayres, D. S.; Baller, B.; et al

    2015-10-20

    Our paper describes the hardware and operations of the Neutrinos at the Main Injector (NuMI) beam at Fermilab. It elaborates on the design considerations for the beam as a whole and for individual elements. The most important part of our design details pertaining to individual components is described. Beam monitoring systems and procedures, including the tuning and alignment of the beam and NuMI long-term performance, are also discussed.

  13. The NuMI neutrino beam

    NASA Astrophysics Data System (ADS)

    Adamson, P.; Anderson, K.; Andrews, M.; Andrews, R.; Anghel, I.; Augustine, D.; Aurisano, A.; Avvakumov, S.; Ayres, D. S.; Baller, B.; Barish, B.; Barr, G.; Barrett, W. L.; Bernstein, R. H.; Biggs, J.; Bishai, M.; Blake, A.; Bocean, V.; Bock, G. J.; Boehnlein, D. J.; Bogert, D.; Bourkland, K.; Cao, S. V.; Castromonte, C. M.; Childress, S.; Choudhary, B. C.; Coelho, J. A. B.; Cobb, J. H.; Corwin, L.; Crane, D.; Cravens, J. P.; Cronin-Hennessy, D.; Ducar, R. J.; De Jong, J. K.; Devan, A. V.; Devenish, N. E.; Diwan, M. V.; Erwin, A. R.; Escobar, C. O.; Evans, J. J.; Falk, E.; Feldman, G. J.; Fields, T. H.; Ford, R.; Frohne, M. V.; Gallagher, H. R.; Garkusha, V.; Gomes, R. A.; Goodman, M. C.; Gouffon, P.; Graf, N.; Gran, R.; Grossman, N.; Grzelak, K.; Habig, A.; Hahn, S. R.; Harding, D.; Harris, D.; Harris, P. G.; Hartnell, J.; Hatcher, R.; Hays, S.; Heller, K.; Holin, A.; Huang, J.; Hylen, J.; Ibrahim, A.; Indurthy, D.; Irwin, G. M.; Isvan, Z.; Jaffe, D. E.; James, C.; Jensen, D.; Johnstone, J.; Kafka, T.; Kasahara, S. M. S.; Koizumi, G.; Kopp, S.; Kordosky, M.; Kreymer, A.; Lang, K.; Laughton, C.; Lefeuvre, G.; Ling, J.; Litchfield, P. J.; Loiacono, L.; Lucas, P.; Mann, W. A.; Marchionni, A.; Marshak, M. L.; Mayer, N.; McGivern, C.; Medeiros, M. M.; Mehdiyev, R.; Meier, J. R.; Messier, M. D.; Michael, D. G.; Milburn, R. H.; Miller, J. L.; Miller, W. H.; Mishra, S. R.; Moed Sher, S.; Moore, C. D.; Morfín, J.; Mualem, L.; Mufson, S.; Murgia, S.; Murtagh, M.; Musser, J.; Naples, D.; Nelson, J. K.; Newman, H. B.; Nichol, R. J.; Nowak, J. A.; O`Connor, J.; Oliver, W. P.; Olsen, M.; Orchanian, M.; Osprey, S.; Pahlka, R. B.; Paley, J.; Para, A.; Patterson, R. B.; Patzak, T.; Pavlović, Ž.; Pawloski, G.; Perch, A.; Peterson, E. A.; Petyt, D. A.; Pfützner, M. M.; Phan-Budd, S.; Plunkett, R. K.; Poonthottathil, N.; Prieto, P.; Pushka, D.; Qiu, X.; Radovic, A.; Rameika, R. A.; Ratchford, J.; Rebel, B.; Reilly, R.; Rosenfeld, C.; Rubin, H. A.; Ruddick, K.; Sanchez, M. C.; Saoulidou, N.; Sauer, L.; Schneps, J.; Schoo, D.; Schreckenberger, A.; Schreiner, P.; Shanahan, P.; Sharma, R.; Smart, W.; Smith, C.; Sousa, A.; Stefanik, A.; Tagg, N.; Talaga, R. L.; Tassotto, G.; Thomas, J.; Thompson, J.; Thomson, M. A.; Tian, X.; Timmons, A.; Tinsley, D.; Tognini, S. C.; Toner, R.; Torretta, D.; Trostin, I.; Tzanakos, G.; Urheim, J.; Vahle, P.; Vaziri, K.; Villegas, E.; Viren, B.; Vogel, G.; Webber, R. C.; Weber, A.; Webb, R. C.; Wehmann, A.; White, C.; Whitehead, L.; Whitehead, L. H.; Wojcicki, S. G.; Wong-Squires, M. L.; Yang, T.; Yumiceva, F. X.; Zarucheisky, V.; Zwaska, R.

    2016-01-01

    This paper describes the hardware and operations of the Neutrinos at the Main Injector (NuMI) beam at Fermilab. It elaborates on the design considerations for the beam as a whole and for individual elements. The most important design details of individual components are described. Beam monitoring systems and procedures, including the tuning and alignment of the beam and NuMI long-term performance, are also discussed.

  14. [Evaluation of a Legionella outbreak emerged in a recently opening hotel].

    PubMed

    Erdoğan, Haluk; Arslan, Hande

    2013-04-01

    Legionnaires' disease (LD) is a systemic infection caused by Legionella species especially colonized in the water systems. Hotels are common locations in which waterwork-associated sporadic or epidemic legionellosis can be detected. The aim of this study was to evaluate a small Legionella outbreak emerged in a recently opened 600-bed hotel in Alanya, a touristic county in Mediterranean part of Turkey. A 66 years old male patient who stayed in this hotel opened on May 15th, 2009, was admitted to our hospital on May 21st, 2009 with the complaints of high fever, headache and diarrhea lasting for three days. Since chest X-ray revealed non-homogenous density increase in left middle and inferior zone, the patient was diagnosed as atypical pneumoniae and LD was confirmed by positive urinary Legionella antigen test (Card test, BinaxNOW®Legionella Urinary Antigen Test; Alere Co, USA) result. Following the identification of the index case, the records of our hospital were reviewed and revealed another case being treated with the diagnosis of community acquired pneumonia who was also the guest of the same hotel. This patient was then diagnosed as LD by positive urinary antigen test. Since new cases were identified during the following days (May 22, 25 and 26) the Antalya County Health Department and hotel management were informed about a cluster of LD. In addition subsequent investigation for environmental surveillance and water sampling were conducted. The LD diagnosis and environmental inspections were performed according to the procedure described in the guideline from "Turkish Ministry of Health Travel-Associated Legionnaires' Disease Control Programme". Five definitive cases and one presumptive case of LD were identified during the outbreak period (May 20-26, 2009). All of the cases were successfully treated (intravenous ciprofloxacin or levofloxacin or clarithromycin), however one patient died due to sudden death during sleep after being discharged. Since sputum

  15. Tumor-suppressing roles of miR-214 and miR-218 in breast cancer.

    PubMed

    Liu, Bo; Tian, Yanfeng; Li, Fang; Zhao, Zengren; Jiang, Xia; Zhai, Congjie; Han, Xiaodong; Zhang, Like

    2016-06-01

    MicroRNAs (miRNAs) are small non-coding RNAs that are key post-transcriptional regulators of gene expression. MicroRNA-214 (miR-214) and microRNA-218 (miR-218) have shown the function of tumor suppressors in various types of human cancers. However, the biological functions of miR-214 and miR-218 in breast cancer have not been elucidated completely. The present study evaluated the expression and biological function of miR-214 and miR-218 in human breast cancer. Our results revealed that the expression of miR-214 and miR-218 were significantly decreased in breast cancer tissues compared with adjacent tissues. The aberrant expression of miR-214 and miR-218 were negatively associated with Ki-67, and the miR-218 expression was positively associated with progesterone receptor (PR) in breast cancer tissues. In vitro, the cell proliferation and migration were decreased, cell apoptosis was induced, and cell cycle was also disturbed in miR-214 or miR-218 overexpressed breast cancer cells. Our results demonstrated that miR-214 and miR-218 function as tumor suppressors in breast cancer, and may become biomarkers and potential therapeutic targets in breast cancer. PMID:27109339

  16. Tumor-suppressing roles of miR-214 and miR-218 in breast cancer

    PubMed Central

    LIU, BO; TIAN, YANFENG; LI, FANG; ZHAO, ZENGREN; JIANG, XIA; ZHAI, CONGJIE; HAN, XIAODONG; ZHANG, LIKE

    2016-01-01

    MicroRNAs (miRNAs) are small non-coding RNAs that are key post-transcriptional regulators of gene expression. MicroRNA-214 (miR-214) and microRNA-218 (miR-218) have shown the function of tumor suppressors in various types of human cancers. However, the biological functions of miR-214 and miR-218 in breast cancer have not been elucidated completely. The present study evaluated the expression and biological function of miR-214 and miR-218 in human breast cancer. Our results revealed that the expression of miR-214 and miR-218 were significantly decreased in breast cancer tissues compared with adjacent tissues. The aberrant expression of miR-214 and miR-218 were negatively associated with Ki-67, and the miR-218 expression was positively associated with progesterone receptor (PR) in breast cancer tissues. In vitro, the cell proliferation and migration were decreased, cell apoptosis was induced, and cell cycle was also disturbed in miR-214 or miR-218 overexpressed breast cancer cells. Our results demonstrated that miR-214 and miR-218 function as tumor suppressors in breast cancer, and may become biomarkers and potential therapeutic targets in breast cancer. PMID:27109339

  17. Telomere Length, TERT, and miRNA Expression.

    PubMed

    Slattery, Martha L; Herrick, Jennifer S; Pellatt, Andrew J; Wolff, Roger K; Mullany, Lila E

    2016-01-01

    It has been proposed that miRNAs are involved in the control of telomeres. We test that hypothesis by examining the association between miRNAs and telomere length (TL). Additionally, we evaluate if genetic variation in telomerase reverse transcriptase (TERT) is associated with miRNA expression levels. We use data from a population-based study of colorectal cancer (CRC), where we have previously shown associations between TL and TERT and CRC, to test associations between TL and miRNA expression and TERT and miRNA expression. To gain insight into functions of miRNAs associated with TERT we tested linear associations between miRNAs and their targeted gene mRNAs. An Agilent platform that contained information on over 2000 miRNAs was used. TL was measured using a multiplexed quantitative PCR (qPCR). RNAseq was used to assess gene expression. Our sample consisted of 1152 individuals with SNP data and miRNA expression data; 363 individuals with both TL and miRNA; and 148 individuals with miRNA and mRNA data. Thirty-three miRNAs were directly associated with TL after adjusting for age and sex (false discovery rate (FDR) of 0.05). TERT rs2736118 was associated with differences in miRNA expression between carcinoma and normal colonic mucosa for 75 miRNAs (FDR <0.05). Genes regulated by these miRNAs, as indicated by mRNA/miRNA associations, were associated with major signaling pathways beyond their TL-related functions, including PTEN, and PI3K/AKT signaling. Our data support a direct association between miRNAs and TL; differences in miRNA expression levels by TERT genotype were observed. Based on miRNA and targeted mRNA associations our data suggest that TERT is involved in non-TL-related functions by acting through altered miRNA expression. PMID:27627813

  18. Structure and activity of putative intronic miRNA promoters.

    PubMed

    Monteys, Alex Mas; Spengler, Ryan M; Wan, Ji; Tecedor, Luis; Lennox, Kimberly A; Xing, Yi; Davidson, Beverly L

    2010-03-01

    MicroRNAs (miRNAs) are RNA sequences of approximately 22 nucleotides that mediate post-transcriptional regulation of specific mRNAs. miRNA sequences are dispersed throughout the genome and are classified as intergenic (between genes) or intronic (embedded into a gene). Intergenic miRNAs are expressed by their own promoter, and until recently, it was supposed that intronic miRNAs are transcribed from their host gene. Here, we performed a genomic analysis of currently known intronic miRNA regions and observed that approximately 35% of intronic miRNAs have upstream regulatory elements consistent with promoter function. Among all intronic miRNAs, 30% have associated Pol II regulatory elements, including transcription start sites, CpG islands, expression sequence tags, and conserved transcription factor binding sites, while 5% contain RNA Pol III regulatory elements (A/B box sequences). We cloned intronic regions encompassing miRNAs and their upstream Pol II (miR-107, miR-126, miR-208b, miR-548f-2, miR-569, and miR-590) or Pol III (miR-566 and miR-128-2) sequences into a promoterless plasmid, and confirmed that miRNA expression occurs independent of host gene transcription. For miR-128-2, a miRNA overexpressed in acute lymphoblastic leukemia, ChIP analysis suggests dual regulation by both intronic (Pol III) and host gene (Pol II) promoters. These data support complex regulation of intronic miRNA expression, and have relevance to disregulation in disease settings. PMID:20075166

  19. Addendum to NuMI shielding assessment

    SciTech Connect

    Vaziri, Kamran; /Fermilab

    2007-10-01

    The original safety assessment and the Safety Envelope for the NuMI beam line corresponds to 400 kW of beam power. The Main Injector is currently capable of and approved for producing 500 kW of beam power2. However, operation of the NuMI beam line at 400 kW of power brings up the possibility of an occasional excursion above 400 kW due to better than usual tuning in one of the machines upstream of the NuMI beam line. An excursion above the DOE approved Safety Envelope will constitute a safety violation. The purpose of this addendum is to evaluate the radiological issues and modifications required to operate the NuMI beam line at 500 kW. This upgrade will allow 400 kW operations with a reasonable safety margin. Configuration of the NuMI beam line, boundaries, safety system and the methodologies used for the calculations are as described in the original NuMI SAD. While most of the calculations presented in the original shielding assessment were based on Monte Carlo simulations, which were based on the design geometries, most of the results presented in this addendum are based on the measurements conducted by the AD ES&H radiation safety group.

  20. Prediction of miRNA targets.

    PubMed

    Oulas, Anastasis; Karathanasis, Nestoras; Louloupi, Annita; Pavlopoulos, Georgios A; Poirazi, Panayiota; Kalantidis, Kriton; Iliopoulos, Ioannis

    2015-01-01

    Computational methods for miRNA target prediction are currently undergoing extensive review and evaluation. There is still a great need for improvement of these tools and bioinformatics approaches are looking towards high-throughput experiments in order to validate predictions. The combination of large-scale techniques with computational tools will not only provide greater credence to computational predictions but also lead to the better understanding of specific biological questions. Current miRNA target prediction tools utilize probabilistic learning algorithms, machine learning methods and even empirical biologically defined rules in order to build models based on experimentally verified miRNA targets. Large-scale protein downregulation assays and next-generation sequencing (NGS) are now being used to validate methodologies and compare the performance of existing tools. Tools that exhibit greater correlation between computational predictions and protein downregulation or RNA downregulation are considered the state of the art. Moreover, efficiency in prediction of miRNA targets that are concurrently verified experimentally provides additional validity to computational predictions and further highlights the competitive advantage of specific tools and their efficacy in extracting biologically significant results. In this review paper, we discuss the computational methods for miRNA target prediction and provide a detailed comparison of methodologies and features utilized by each specific tool. Moreover, we provide an overview of current state-of-the-art high-throughput methods used in miRNA target prediction. PMID:25577381

  1. Energy End-Use Patterns in Full-Service Hotels: A Case Study

    SciTech Connect

    Placet, Marylynn; Katipamula, Srinivas; Liu, Bing; Dirks, James A.; Xie, YuLong; Sullivan, Greg; Walent, Jim; Williamson, Rebecca

    2010-06-30

    The U.S. Department of Energy (DOE) recently initiated a program -- Commercial Building Partnerships (CBP) -- to work with private-sector companies in the design of highly-efficient retrofit and new construction projects. Pacific Northwest National Laboratory (PNNL) is conducting a project with a major hotel company to retrofit a full-service, large hotel with the goal of reducing energy consumption by at least 30%. The first step of the project was an intensive metering and monitoring effort aimed at understanding energy end use patterns in the hotel. About 10% of the guest rooms (32), as well as circuits for most of the end uses in public spaces (lighting, elevators, air handlers and other HVAC system components, and various equipment), were equipped with meters. Data are being collected at 1- or 5-minute intervals and downloaded on a monthly basis for analysis. This paper presents results from the first four months of the monitoring effort, which revealed energy end-use consumption patterns, variability of guest room energy use, daily load curves, monthly variations, and other aspects of hotel energy use. Metered end-use data for hotels at this level of detail are not available from any currently-available public sources. This study presents unique information and insight into energy end-use patterns in the lodging sector of commercial buildings and can also serve as a case study of a complex sub-metering project.

  2. Spatio-temporal Hotelling observer for signal detection from image sequences

    PubMed Central

    Caucci, Luca; Barrett, Harrison H.; Rodríguez, Jeffrey J.

    2010-01-01

    Detection of signals in noisy images is necessary in many applications, including astronomy and medical imaging. The optimal linear observer for performing a detection task, called the Hotelling observer in the medical literature, can be regarded as a generalization of the familiar prewhitening matched filter. Performance on the detection task is limited by randomness in the image data, which stems from randomness in the object, randomness in the imaging system, and randomness in the detector outputs due to photon and readout noise, and the Hotelling observer accounts for all of these effects in an optimal way. If multiple temporal frames of images are acquired, the resulting data set is a spatio-temporal random process, and the Hotelling observer becomes a spatio-temporal linear operator. This paper discusses the theory of the spatio-temporal Hotelling observer and estimation of the required spatio-temporal covariance matrices. It also presents a parallel implementation of the observer on a cluster of Sony PLAYSTATION 3 gaming consoles. As an example, we consider the use of the spatio-temporal Hotelling observer for exoplanet detection. PMID:19550494

  3. Spatio-temporal Hotelling observer for signal detection from image sequences.

    PubMed

    Caucci, Luca; Barrett, Harrison H; Rodriguez, Jeffrey J

    2009-06-22

    Detection of signals in noisy images is necessary in many applications, including astronomy and medical imaging. The optimal linear observer for performing a detection task, called the Hotelling observer in the medical literature, can be regarded as a generalization of the familiar prewhitening matched filter. Performance on the detection task is limited by randomness in the image data, which stems from randomness in the object, randomness in the imaging system, and randomness in the detector outputs due to photon and readout noise, and the Hotelling observer accounts for all of these effects in an optimal way. If multiple temporal frames of images are acquired, the resulting data set is a spatio-temporal random process, and the Hotelling observer becomes a spatio-temporal linear operator. This paper discusses the theory of the spatio-temporal Hotelling observer and estimation of the required spatio-temporal covariance matrices. It also presents a parallel implementation of the observer on a cluster of Sony PLAYSTATION 3 gaming consoles. As an example, we consider the use of the spatio-temporal Hotelling observer for exoplanet detection. PMID:19550494

  4. Economic analysis of the water demand in the hotels and restaurants sector: Shadow prices and elasticities

    NASA Astrophysics Data System (ADS)

    Angulo, Ana; Atwi, Majed; Barberán, Ramón; Mur, Jesús

    2014-08-01

    Despite the growing economic importance of tourism, and its impact on relative water shortage, little is known about the role that water plays in the productive process of hotels and restaurants and, therefore, the possible implications of water demand management policy for this sector. This study aims to fill this gap. It is based on the microdata of 676 firms in the sector, operating in the city of Zaragoza (Spain) for a 12 year period. Based on the Translog cost function, we estimate the shadow price of water in the short run and, from a long-run perspective, its direct price elasticity, its cross elasticities relative to labor, capital, and supplies, and its elasticity with respect to the level of output. The results obtained show that water provides sector firms returns that are on average higher than its price, although in the case of hotels the margin is really narrow. This situation provides policy makers with a margin for applying price increases without affecting the sector's viability, with some caution in the case of hotels. Water demand elasticity equals -0.38 in the case of hotels, but it is not significant in the case of restaurants and bar-cafes; hence, only in hotels is there potential for influencing water use patterns, encouraging the resource's conservation through pricing policy. Moreover, capital is a substitutive factor of water, and the elasticity of water with respect to output is 0.40, all of which should also be considered by policy makers in water resource management.

  5. Aberrant plasma levels of circulating miR-16, miR-107, miR-130a and miR-146a are associated with lymph node metastasis and receptor status of breast cancer patients

    PubMed Central

    Stückrath, Isabel; Rack, Brigitte; Janni, Wolfgang; Jäger, Bernadette; Pantel, Klaus; Schwarzenbach, Heidi

    2015-01-01

    Within the multicenter SUCCESS trial, we investigated the association of plasma microRNAs with different subtypes of invasive breast cancer. Six miRs (miR-16, miR-27a, miR-107, miR-130a, miR-132 and miR-146a) were selected from microarray profiling and further validated in plasma of 111 breast cancer patients before and after chemotherapy and 46 healthy women by quantitative real-time PCR. Plasma levels of miR-16 (p = 0.0001), miR-27a (p = 0.039) and miR-132 (p = 0.020) were higher in breast cancer patients before chemotherapy than healthy women. With the exception of miR-16, the increased levels of miR-27a (p = 0.035) and miR-132 (p = 0.025) decreased after chemotherapy to those observed in healthy women. Levels of miR-16 (p = 0.019), miR-107 (p = 0.036), miR-130a (p = 0.027) and miR-146a (p = 0.047) were different between lymph node -positive and -negative patients, while the levels of miR-130a (p = 0.001) and miR-146a (p = 0.025) also differed between HER2-positive and -negative status. Estrogen-receptor negative tumors displayed higher concentrations of circulating miR-107 than their counterparts (p = 0.035). However, overexpression of miR-107 in MCF-7 cells did not downregulate estrogen receptor protein. Altered expression levels of miR-107 influenced the migration and invasion behavior of MCF-7 and MDA-MB-231 cells. Our data indicate differential concentrations of plasma miR-16, miR-107, miR-130a and miR-146a in different breast cancer subtypes, suggesting a potential role of these miRs in breast cancer biology and tumor progression. PMID:26033453

  6. Aberrant plasma levels of circulating miR-16, miR-107, miR-130a and miR-146a are associated with lymph node metastasis and receptor status of breast cancer patients.

    PubMed

    Stückrath, Isabel; Rack, Brigitte; Janni, Wolfgang; Jäger, Bernadette; Pantel, Klaus; Schwarzenbach, Heidi

    2015-05-30

    Within the multicenter SUCCESS trial, we investigated the association of plasma microRNAs with different subtypes of invasive breast cancer.Six miRs (miR-16, miR-27a, miR-107, miR-130a, miR-132 and miR-146a) were selected from microarray profiling and further validated in plasma of 111 breast cancer patients before and after chemotherapy and 46 healthy women by quantitative real-time PCR.Plasma levels of miR-16 (p = 0.0001), miR-27a (p = 0.039) and miR-132 (p = 0.020) were higher in breast cancer patients before chemotherapy than healthy women. With the exception of miR-16, the increased levels of miR-27a (p = 0.035) and miR-132 (p = 0.025) decreased after chemotherapy to those observed in healthy women. Levels of miR-16 (p = 0.019), miR-107 (p = 0.036), miR-130a (p = 0.027) and miR-146a (p = 0.047) were different between lymph node -positive and -negative patients, while the levels of miR-130a (p = 0.001) and miR-146a (p = 0.025) also differed between HER2-positive and -negative status. Estrogen-receptor negative tumors displayed higher concentrations of circulating miR-107 than their counterparts (p = 0.035). However, overexpression of miR-107 in MCF-7 cells did not downregulate estrogen receptor protein. Altered expression levels of miR-107 influenced the migration and invasion behavior of MCF-7 and MDA-MB-231 cells.Our data indicate differential concentrations of plasma miR-16, miR-107, miR-130a and miR-146a in different breast cancer subtypes, suggesting a potential role of these miRs in breast cancer biology and tumor progression. PMID:26033453

  7. Aberrant expression of miR-127, miR-21 and miR-16 in placentas of deceased cloned sheep.

    PubMed

    Ni, Wei; You, Shuang; Cao, Yang; Li, Cunyuan; Wei, Junchuang; Wang, Dawei; Qiao, Jun; Zhao, Xinxia; Hu, Shengwei; Quan, Renzhe

    2016-04-01

    Placental deficiencies are associated with developmental abnormalities of animal produced by somatic cell nuclear transfer (SCNT). It is reported that aberrant expression of microRNAs (miRNAs) in the common placenta is associated with fetal growth restriction and placental deficiencies. However, an understanding of the expression and function of miRNAs in the placentas of cloned animal is lacking. In this study, we characterized the expression of five growth-associated miRNAs (miR-127, miR-16, miR-21, miR-93 and miR-182) in placentas of deceased transgenic cloned sheep (deceased group, n=7), live transgenic cloned sheep (live group, n=5) and conventionally produced sheep (control group, n=10). Expression levels of miR-127 (P<0.01), miR-21 (P<0.01) and miR-16 (P<0.05) were significantly up-regulated in the placentas of deceased group compared to that of control group. In contrast, the expression of these miRNAs was largely normal in the placentas of live group, except for the expression of miR-21. Furthermore, we confirmed that retrotransposon-like gene (Rtl1), a key gene in placental development, was down-regulated by miR-127 as a target in placenta cells. Our results suggested that the abnormal expression of miR-127, miR-21 and miR-16 in placentas of deceased sheep, through dysregulation of target genes, may result in developmental deficiencies of transgenic cloned sheep. PMID:27033933

  8. Misprocessing and functional arrest of microRNAs by miR-Pirate: roles of miR-378 and miR-17.

    PubMed

    Deng, Zhaoqun; Yang, Xiangling; Fang, Ling; Rutnam, Zina J; Yang, Burton B

    2013-03-01

    miRNAs (microRNAs) are short non-coding RNAs that can regulate gene expression in cancer development, which makes them valuable targets for therapeutic intervention. In the present study we report on an approach that can not only arrest the functions of mature miRNAs by binding to them, but it can also induce the 'mis-processing' of the target miRNA, producing a non-functional truncated miRNA. This approach involves generating an expression construct that produces an RNA fragment with 16 repeat sequences. The construct is named miR-Pirate (miRNA-interacting RNA-producing imperfect RNA and tangling endogenous miRNA). The transcript of the construct contained mismatches to the seed region, and thus it would not target the potential targets of the miRNA under study. The homology of the construct is sufficiently high, allowing the transcript to block miRNA functions. The functions of the construct were validated in cell cultures, in tumour formation assays and in transgenic mice stably expressing this construct. To explore the possibility of adopting this approach in gene therapy, we transfected cells with synthetic miR-Pirate and obtained the results we expected. The miR-Pirate, expressed by the construct or synthesized chemically, was found to be able to specifically pirate and silence a mature miRNA through its dual roles and thus could be clinically applied for miRNA intervention. PMID:23210454

  9. Role of miR-1 and miR-133a in myocardial ischemic postconditioning

    PubMed Central

    2011-01-01

    Background Ischemic postconditioning (IPost) has aroused much attention since 2003 when it was firstly reported. The role of microRNAs (miRNAs or miRs) in IPost has rarely been reported. The present study was undertaken to investigate whether miRNAs were involved in the protective effect of IPost against myocardial ischemia-reperfusion (IR) injury and the probable mechanisms involved. Methods Thirty SD rats weighing 250-300 g were equally randomized to three groups: Control group, where the rats were treated with thoracotomy only; IR group, where the rats were treated with ischemia for 60 min and reperfusion for 180 min; and IPost group, where the rats were treated with 3 cycles of transient IR just before reperfusion. The extent of myocardial infarction, LDH and CK activities were measured immediately after treatment. Myocardial apoptosis was detected by TUNEL assay. The myocardial tissue was collected after IR or IPost stimulation to evaluate the miRNAs expression level by miRNA-microarray and quantitative real-time RT-PCR. Real-time PCR was conducted to identify changes in mRNA expression of apoptosis-related genes such as Bcl-2, Bax and Caspase-9 (CASP9), and Western blot was used to compare the protein expression level of CASP9 in the three groups. The miRNA mimics and anti-miRNA oligonucleotides (AMO) were transferred into the cultured neonatal cardiomyocytes and myocardium before they were treated with IR. The effect of miRNAs on apoptosis was determined by flow cytometry and TUNEL assay. CASP9, as one of the candidate target of miR-133a, was compared during IR after the miR-133a mimic or AMO-133a was transferred into the myocardium. Results IPost reduced the IR-induced infarct size of the left ventricle, and decreased CK and LDH levels. TUNEL assay showed that myocardial apoptosis was attenuated by IPost compared with IR. MiRNA-microarray and RT-PCR showed that myocardial-specific miR-1 and miR-133a were down-regulated by IR, and up-regulated by IPost

  10. Circulating miRNAs as Potential Marker for Pulmonary Hypertension

    PubMed Central

    Wei, Chuanyu; Henderson, Heather; Spradley, Christopher; Li, Li; Kim, Il-Kwon; Kumar, Sandeep; Hong, Nayeon; Arroliga, Alejandro C.; Gupta, Sudhiranjan

    2013-01-01

    MircoRNAs (miRNAs) are small non-coding RNAs that govern the gene expression and, play significant role in the pathogenesis of heart failure. The detection of miRNAs in circulation of pulmonary hypertensive (PH) human subjects remains elusive. In the current study, we determined the pattern of miRNAs of mild-to-severe human PH subjects and, compared them with the control subjects by miRNA array. Blood was obtained using fluoroscopic and waveform guided catheterization from the distal (pulmonary artery) port of the catheter. A total 40 human subjects were included in the study and, the degree of PH was determined by mean pulmonary arterial pressure. Among several miRNAs in the array, we validated 14 miRNAs and, the data were consistent with the array profile. We identified several novel downregulated miRNAs (miR-451, miR-1246) and upregulated miRNAs (miR-23b, miR-130a and miR-191) in the circulation of PH subjects. Our study showed novel set of miRNAs which are dysregulated in PH and, are directly proportional to the degree of PH. These miRNAs may be considered as potential biomarker for early detection of PH. PMID:23717609

  11. Meeting Competence Needs in the Hotel and Catering Industry Now and in the Future. Research Report 1992.

    ERIC Educational Resources Information Center

    Walker, Anne, Ed.; Hayter, Roy, Ed.

    The hotel and catering industry in the United Kingdom is estimated to have employed 2,392,000 people in 1990--about 9 percent of the total work force in employment in the country. These figures came from a major study into the skills needs of the hotel and catering industry in relation to the current provision of vocational education. Data are…

  12. Converging miRNA functions in diverse brain disorders: A case for miR-124 and miR-126

    PubMed Central

    Sonntag, Kai C.; Woo, Tsung-Ung W.; Krichevsky, Anna M.

    2012-01-01

    A growing body of information on the biology of miRNAs has revealed new insight into their roles in normal homeostasis and pathology of disease. miRNAs control all steps of the cellular expression machinery acting through a “single miRNA/multiple targets” or “multiple miRNAs/single target” mechanism. They have profound impact on the regulation of signaling pathways, which govern common and specific functions across different cellular phenotypes. There is increasing evidence that various diseases share similar disturbances in gene expression networks. Since miRNAs have both common and varying effects in different cellular contexts, they might also influence overlapping signaling pathways in different organs and disease entities. Here, we review this concept for two miRNAs highly abundant in the brain, miR-124 and miR-126, and their potential role in diseases of the brain. PMID:22178324

  13. CCAR1 5′ UTR as a natural miRancer of miR-1254 overrides tamoxifen resistance

    PubMed Central

    Li, Gaopeng; Wu, Xiaoli; Qian, Wenchang; Cai, Huayong; Sun, Xinbao; Zhang, Weijie; Tan, Sheng; Wu, Zhengsheng; Qian, Pengxu; Ding, Keshuo; Lu, Xuefei; Zhang, Xiao; Yan, Hong; Song, Haifeng; Guang, Shouhong; Wu, Qingfa; Lobie, Peter E; Shan, Ge; Zhu, Tao

    2016-01-01

    MicroRNAs (miRNAs) typically bind to unstructured miRNA-binding sites in target RNAs, leading to a mutual repression of expression. Here, we report that miR-1254 interacts with structured elements in cell cycle and apoptosis regulator 1 (CCAR1) 5′ untranslated region (UTR) and this interaction enhances the stability of both molecules. miR-1254 can also act as a repressor when binding to unstructured sites in its targets. Interestingly, structured miR-1254-targeting sites act as both a functional RNA motif-sensing unit, and an independent RNA functional unit that enhances miR-1254 expression. Artificially designed miRNA enhancers, termed “miRancers”, can stabilize and enhance the activity of miRNAs of interest. We further demonstrate that CCAR1 5′ UTR as a natural miRancer of endogenous miR-1254 re-sensitizes tamoxifen-resistant breast cancer cells to tamoxifen. Thus, our study presents a novel model of miRNA function, wherein highly structured miRancer-like motif-containing RNA fragments or miRancer molecules specifically interact with miRNAs, leading to reciprocal stabilization. PMID:27002217

  14. CCAR1 5' UTR as a natural miRancer of miR-1254 overrides tamoxifen resistance.

    PubMed

    Li, Gaopeng; Wu, Xiaoli; Qian, Wenchang; Cai, Huayong; Sun, Xinbao; Zhang, Weijie; Tan, Sheng; Wu, Zhengsheng; Qian, Pengxu; Ding, Keshuo; Lu, Xuefei; Zhang, Xiao; Yan, Hong; Song, Haifeng; Guang, Shouhong; Wu, Qingfa; Lobie, Peter E; Shan, Ge; Zhu, Tao

    2016-06-01

    MicroRNAs (miRNAs) typically bind to unstructured miRNA-binding sites in target RNAs, leading to a mutual repression of expression. Here, we report that miR-1254 interacts with structured elements in cell cycle and apoptosis regulator 1 (CCAR1) 5' untranslated region (UTR) and this interaction enhances the stability of both molecules. miR-1254 can also act as a repressor when binding to unstructured sites in its targets. Interestingly, structured miR-1254-targeting sites act as both a functional RNA motif-sensing unit, and an independent RNA functional unit that enhances miR-1254 expression. Artificially designed miRNA enhancers, termed "miRancers", can stabilize and enhance the activity of miRNAs of interest. We further demonstrate that CCAR1 5' UTR as a natural miRancer of endogenous miR-1254 re-sensitizes tamoxifen-resistant breast cancer cells to tamoxifen. Thus, our study presents a novel model of miRNA function, wherein highly structured miRancer-like motif-containing RNA fragments or miRancer molecules specifically interact with miRNAs, leading to reciprocal stabilization. PMID:27002217

  15. Work Conditions and Health and Well-Being of Latina Hotel Housekeepers.

    PubMed

    Hsieh, Yu-Chin; Apostolopoulos, Yorghos; Sönmez, Sevil

    2016-06-01

    Hotel housekeepers are exposed to a plethora of disproportionately high work-induced hazards that can lead to adverse health consequences. Latina hotel housekeepers are rendered particularly vulnerable to elevated occupational hazards and resultant health strains due to their socioeconomic status, immigration status, language barriers, and lack of access to healthcare services. The findings from the 27 interviews with Latina hotel housekeepers indicated that the interviewees were exposed to physical, chemical, and social hazards in the workplace and suffered musculoskeletal injuries. In terms of psychological wellness, the time pressure of cleaning rooms quickly and work-related stress stemming from workplace mistreatment emerged as major work-related stressors. Recommendations are made for the introduction of multilevel interventions designed to prevent work-related injuries and illnesses and to promote healthier workplaces. PMID:26001842

  16. [Duration of sick-leave and the moment of recovery in the hotel industry].

    PubMed

    Folgerø, I S; Larsen, S

    1991-09-10

    The paper addresses the problem of sick leave in the hotel industry. It was hypothesized that there would be a tendency for granted sick leaves to include weekends, thus imposing an additional financial burden on the hotel in question. A total of 401 medical certificates from a large city hotel were reviewed. According to these certificates one third of the patients regained their health between Sunday and Monday. Of the patients with "short" sick leaves (less than a fortnight), 40% recovered between Sunday and Monday. The average duration of the sick leave for this group was 1.3 days longer than that of the patients whose sick leaves ended on other days of the week. The results are discussed in terms of the ambiguous position of the medical practitioner. PMID:1948856

  17. Robust Hotelling T2 control chart using reweighted minimum vector variance estimators

    NASA Astrophysics Data System (ADS)

    Ali, Hazlina; Yahaya, Sharipah Soaad Syed; Omar, Zurni

    2014-12-01

    Hotelling T2 control chart is employed to monitor the stability of a multivariate process in Phase I and II. Traditional Hotelling T2 control chart using classical estimators in Phase I, however, suffers from masking and swamping effects and thus jeorpadizes its performance. To alleviate this problem, robust location and scale estimators are recommended instead. In this paper, a new Hotelling T2 control chart based on highly robust and efficient estimators of location and scatter estimators, known as reweighted minimum vector variance estimators, is proposed. Numerical results show that the new chart is not only capable of detecting outliers but it can also control the alarm rates better than the existing charts.

  18. A Room with a Viewpoint Revisited: Descriptive Norms and Hotel Guests' Towel Reuse Behavior

    PubMed Central

    Bohner, Gerd; Schlüter, Lena E.

    2014-01-01

    Field experiments on descriptive norms as a means to increase hotel guests' towel reuse [1] were replicated and extended. In two hotels in Germany (Study 1: N = 724; Study 2: N = 204), descriptive norm messages suggesting that 75% of guests had reused their towels, or a standard message appealing to environmental concerns, were placed in guests' bathrooms. Descriptive norm messages varied in terms of proximity of the reference group (“hotel guests” vs. “guests in this room”) and temporal proximity (currently vs. two years previous). Reuse of towels was unobtrusively recorded. Results showed that reuse rates were high overall and that both standard and descriptive norm messages increased reuse rates compared to a no-message baseline. However, descriptive norm messages were not more effective than the standard message, and effects of proximity were inconsistent across studies. Discussion addresses cultural and conceptual issues in comparing the present findings with previous ones. PMID:25084348

  19. A room with a viewpoint revisited: descriptive norms and hotel guests' towel reuse behavior.

    PubMed

    Bohner, Gerd; Schlüter, Lena E

    2014-01-01

    Field experiments on descriptive norms as a means to increase hotel guests' towel reuse [1] were replicated and extended. In two hotels in Germany (Study 1: N = 724; Study 2: N = 204), descriptive norm messages suggesting that 75% of guests had reused their towels, or a standard message appealing to environmental concerns, were placed in guests' bathrooms. Descriptive norm messages varied in terms of proximity of the reference group ("hotel guests" vs. "guests in this room") and temporal proximity (currently vs. two years previous). Reuse of towels was unobtrusively recorded. Results showed that reuse rates were high overall and that both standard and descriptive norm messages increased reuse rates compared to a no-message baseline. However, descriptive norm messages were not more effective than the standard message, and effects of proximity were inconsistent across studies. Discussion addresses cultural and conceptual issues in comparing the present findings with previous ones. PMID:25084348

  20. Economic Evaluation of a Worksite Obesity Prevention and Intervention Trial among Hotel Workers in Hawaii

    PubMed Central

    Meenan, Richard T.; Vogt, Thomas M.; Williams, Andrew E.; Stevens, Victor J.; Albright, Cheryl L.; Nigg, Claudio

    2010-01-01

    Objective Economic evaluation of Work, Weight, and Wellness (3W), a two-year randomized trial of a weight loss program delivered through Hawaii hotel worksites. Methods Business case analysis from hotel perspective. Program resources were micro-costed (2008 dollars). Program benefits were reduced medical costs, fewer absences, and higher productivity. Primary outcome was discounted 24-month net present value (NPV). Results Control program cost $222K to implement over 24 months ($61 per participant), intervention program cost $1.12M ($334). Including overweight participants (body mass index > 25), discounted control NPV was −$217K; −$1.1M for intervention program. Presenteeism improvement of 50% combined with baseline 10% productivity shortfall required to generate positive 24-month intervention NPV. Conclusions 3W’s positive clinical outcomes did not translate into immediate economic benefit for participating hotels, although modest cost savings were observed in the trial’s second year. PMID:20061889

  1. Close correlation between magnetic properties and the soft phonon mode of the structural transition in <mi>BaFe>2<mi>As>2 and <mi>SrFe>2<mi>As>2

    SciTech Connect

    Parshall, D.; Pintschovius, L.; Niedziela, Jennifer L.; Castellan, J. -P.; Lamago, D.; Mittal, R.; Wolf, Th.; Reznik, Dmitry

    2015-04-27

    Parent compounds of Fe-based superconductors undergo a structural phase transition from a tetragonal to an orthorhombic structure. We investigated the temperature dependence of the frequencies of TA phonons that extrapolate to the shear vibrational mode at the zone center, which corresponds to the orthorhombic deformation of the crystal structure at low temperatures in <mi>BaFe>2<mi>As>2 and <mi>SrFe>2<mi>As>2. We found that acoustic phonons at small wave vectors soften gradually towards the transition from high temperatures, tracking the increase of the size of slowly fluctuating magnetic domains. On cooling below the transition to base temperature the phonons harden, following the square of the magnetic moment (which we find is proportional to the anisotropy gap). Finally, our results provide evidence for close correlation between magnetic and phonon properties in Fe-based superconductors.

  2. The Type I IFN-Induced miRNA, miR-21

    PubMed Central

    Yang, Chuan He; Li, Kui; Pfeffer, Susan R.; Pfeffer, Lawrence M.

    2015-01-01

    The interferon (IFN) family of cytokines not only has antiviral properties at various steps in the viral replication cycle, but also anticancer activity through multiple pathways that include inhibiting cell proliferation, regulating cellular responses to inducers of apoptosis and modulating angiogenesis and the immune system. IFNs are known to induce their biological activity through the induction of protein encoding IFN-stimulated genes. However, recent studies have established that IFNs also induce the expression of microRNAs (miRNAs), which are small endogenous non-coding RNAs that suppress gene expression at the post-transcriptional level. MiRNAs play critical roles in tumorigenesis and have been implicated to act as either oncogenes or tumor suppressors in various human cancers. Therefore, IFN-induced miRNAs play an important role, not only in the host response to innate immune response to cancer, but also in the tumorigenic process itself. Furthermore, IFN-induced miRNAs may participate in and/or orchestrate antiviral defense in certain viral infections. In this review, we describe our recent studies on the induction of miR-21 by type I IFN, the role of the STAT3 and NFκB signaling pathways in IFN-induced miR-21 expression, the role of miR-21 in different cancers and the role of miR-21 in regulating the antiviral response. PMID:26610525

  3. A genome-wide miRNA screen revealed miR-603 as a MGMT-regulating miRNA in glioblastomas

    PubMed Central

    Ng, Kimberly; Steed, Tyler; Nguyen, Thien; Futalan, Diahnn; Akers, Johnny C.; Sarkaria, Jann; Jiang, Tao; Chowdhury, Dipanjan; Carter, Bob S.; Chen, Clark C.

    2014-01-01

    MGMT expression is a critical determinant for therapeutic resistance to DNA alkylating agents. We previously demonstrated that MGMT expression is post-transcriptionally regulated by miR-181d and other miRNAs. Here, we performed a genome-wide screen to identify MGMT regulating miRNAs. Candidate miRNAs were further tested for inverse correlation with MGMT expression in clinical specimens. We identified 15 candidate miRNAs and characterized the top candidate, miR-603. Transfection of miR-603 suppressed MGMT mRNA/protein expression in vitro and in vivo; this effect was reversed by transfection with antimiR-603. miR-603 affinity-precipitated with MGMT mRNA and suppressed luciferase activity in an MGMT-3'UTR-luciferase assay, suggesting direct interaction between miR-603 and MGMT 3'UTR. miR-603 transfection enhanced the temozolomide (TMZ) sensitivity of MGMT-expressing glioblastoma cell lines. Importantly, miR-603 mediated MGMT suppression and TMZ resistance were reversed by expression of an MGMT cDNA. In a collection of 74 clinical glioblastoma specimens, both miR-603 and miR-181d levels inversely correlated with MGMT expression. Moreover, a combined index of the two miRNAs better reflected MGMT expression than each individually. These results suggest that MGMT is co-regulated by independent miRNAs. Characterization of these miRNAs should contribute toward strategies for enhancing the efficacy of DNA alkylating agents. PMID:24994119

  4. Role of miR-27a, miR-181a and miR-20b in gastric cancer hypoxia-induced chemoresistance.

    PubMed

    Danza, Katia; Silvestris, Nicola; Simone, Giovanni; Signorile, Michele; Saragoni, Luca; Brunetti, Oronzo; Monti, Manlio; Mazzotta, Annalisa; De Summa, Simona; Mangia, Anita; Tommasi, Stefania

    2016-04-01

    Despite the search for new therapeutic strategies for gastric cancer (GC), there is much evidence of progression due to resistance to chemotherapy. Multidrug resistance (MDR) is the ability of cancer cells to survive after exposure to chemotherapeutic agents. The involvement of miRNAs in the development of MDR has been well described but miRNAs able to modulate the sensitivity to chemotherapy by regulating hypoxia signaling pathways have not yet been fully addressed in GC. Our aim was to analyze miR-20b, miR-27a and miR-181a expression with respect to (epirubicin/oxaliplatin/capecitabine (EOX)) chemotherapy regimen in a set of GC patients, in order to investigate whether miRNAs deregulation may influence GC MDR also via hypoxia signaling modulation. Cancer biopsy were obtained from 21 untreated HER2 negative advanced GC patients, retrospectively analyzed. All patients received a first-line chemotherapy (EOX) regimen. MirWalk database was used to identify miR-27a, miR-181a and miR-20b target genes. The expression of miRNAs and of HIPK2, HIF1A and MDR1 genes were detected by real-time PCR. HIPK2 localization was assessed by immunohistochemistry. Our data showed the down-regulation of miR-20b, miR-27a, miR-181a concomitantly to higher levels of MDR1, HIF1A and HIPK2 genes in GC patients with a progressive disease respect to those with a disease control rate. Moreover, immunohistochemistry assay highlighted a higher cytoplasmic HIPK2 staining, suggesting a different role for it. We showed that aberrant expression of miR-20b, miR27a and miR-181a was associated with chemotherapeutic response in GC through HIF1A, MDR1 and HIPK2 genes modulation, suggesting a possible novel therapeutic strategy. PMID:26793992

  5. Optimum contracted-for water supply for hotels in arid coastal regions.

    PubMed

    Lamei, A; von Münch, E; van der Zaag, P; Imam, E

    2009-01-01

    Hotels in arid coastal areas use mainly desalinated water for their domestic water demands, and treated wastewater for irrigating green areas. Private water companies supply these hotels with their domestic water needs. There is normally a contractual agreement stating a minimum requirement that has to be supplied by the water company and that the hotel management has to pay for regardless of its actual consumption ("contracted-for water supply"). This paper describes a model to determine what value a hotel should choose for its contracted-for water supply in order to minimize its total annual water costs. An example from an arid coastal tourism-dominated city is presented: Sharm El Sheikh, Egypt.The managers of hotels with expected high occupancy rates (74% and above) can contract for more than 80%. On the other hand, hotels with expected lower occupancy rates (60% and less) can contract for less than 70% of the peak daily domestic water demand. With a green area ratio of 40 m(2)/room or less, an on-site wastewater treatment plant can satisfy the required irrigation demand for an occupancy rate as low as 42%. Increasing the ratio of green irrigated area to 100 m(2)/room does not affect the contracted-for water supply at occupancy rates above 72%; at lower occupancy rates, however, on-site treated wastewater is insufficient for irrigating the green areas. Increasing the green irrigated area to 120 m(2)/room increases the need for additional water, either from externally sourced treated wastewater or potable water. The cost of the former is much lower than the latter (0.58 versus 1.52 to 2.14 US$/m(3) in the case study area). PMID:19403967

  6. Introns of plant pri-miRNAs enhance miRNA biogenesis

    PubMed Central

    Bielewicz, Dawid; Kalak, Malgorzata; Kalyna, Maria; Windels, David; Barta, Andrea; Vazquez, Franck; Szweykowska-Kulinska, Zofia; Jarmolowski, Artur

    2013-01-01

    Plant MIR genes are independent transcription units that encode long primary miRNA precursors, which usually contain introns. For two miRNA genes, MIR163 and MIR161, we show that introns are crucial for the accumulation of proper levels of mature miRNA. Removal of the intron in both cases led to a drop-off in the level of mature miRNAs. We demonstrate that the stimulating effects of the intron mostly reside in the 5′ss rather than on a genuine splicing event. Our findings are biologically significant as the presence of functional splice sites in the MIR163 gene appears mandatory for pathogen-triggered accumulation of miR163 and proper regulation of at least one of its targets. PMID:23681439

  7. Discovery of miRNAs and Their Corresponding miRNA Genes in Atlantic Cod (Gadus morhua): Use of Stable miRNAs as Reference Genes Reveals Subgroups of miRNAs That Are Highly Expressed in Particular Organs

    PubMed Central

    Andreassen, Rune; Rangnes, Fredrik; Sivertsen, Maria; Chiang, Michelle; Tran, Michelle; Worren, Merete Molton

    2016-01-01

    Background Atlantic cod (Gadus morhua) is among the economically most important species in the northern Atlantic Ocean and a model species for studying development of the immune system in vertebrates. MicroRNAs (miRNAs) are an abundant class of small RNA molecules that regulate fundamental biological processes at the post-transcriptional level. Detailed knowledge about a species miRNA repertoire is necessary to study how the miRNA transcriptome modulate gene expression. We have therefore discovered and characterized mature miRNAs and their corresponding miRNA genes in Atlantic cod. We have also performed a validation study to identify suitable reference genes for RT-qPCR analysis of miRNA expression in Atlantic cod. Finally, we utilized the newly characterized miRNA repertoire and the dedicated RT-qPCR method to reveal miRNAs that are highly expressed in certain organs. Results The discovery analysis revealed 490 mature miRNAs (401 unique sequences) along with precursor sequences and genomic location of the miRNA genes. Twenty six of these were novel miRNA genes. Validation studies ranked gmo-miR-17-1—5p or the two-gene combination gmo-miR25-3p and gmo-miR210-5p as most suitable qPCR reference genes. Analysis by RT-qPCR revealed 45 miRNAs with significantly higher expression in tissues from one or a few organs. Comparisons to other vertebrates indicate that some of these miRNAs may regulate processes like growth, lipid metabolism, immune response to microbial infections and scar damage repair. Three teleost-specific and three novel Atlantic cod miRNAs were among the differentially expressed miRNAs. Conclusions The number of known mature miRNAs was considerably increased by our identification of miRNAs and miRNA genes in Atlantic cod. This will benefit further functional studies of miRNA expression using deep sequencing methods. The validation study showed that stable miRNAs are suitable reference genes for RT-qPCR analysis of miRNA expression. Applying RT-qPCR we

  8. Polysome shift assay for direct measurement of miRNA inhibition by anti-miRNA drugs

    PubMed Central

    Androsavich, John R.; Sobczynski, Daniel J.; Liu, Xueqing; Pandya, Shweta; Kaimal, Vivek; Owen, Tate; Liu, Kai; MacKenna, Deidre A.; Chau, B. Nelson

    2016-01-01

    Anti-miRNA (anti-miR) oligonucleotide drugs are being developed to inhibit overactive miRNAs linked to disease. To help facilitate the transition from concept to clinic, new research tools are required. Here we report a novel method—miRNA Polysome Shift Assay (miPSA)—for direct measurement of miRNA engagement by anti-miR, which is more robust than conventional pharmacodynamics using downstream target gene derepression. The method takes advantage of size differences between active and inhibited miRNA complexes. Active miRNAs bind target mRNAs in high molecular weight polysome complexes, while inhibited miRNAs are sterically blocked by anti-miRs from forming this interaction. These two states can be assessed by fractionating tissue or cell lysates using differential ultracentrifugation through sucrose gradients. Accordingly, anti-miR treatment causes a specific shift of cognate miRNA from heavy to light density fractions. The magnitude of this shift is dose-responsive and maintains a linear relationship with downstream target gene derepression while providing a substantially higher dynamic window for aiding drug discovery. In contrast, we found that the commonly used ‘RT-interference’ approach, which assumes that inhibited miRNA is undetectable by RT-qPCR, can yield unreliable results that poorly reflect the binding stoichiometry of anti-miR to miRNA. We also demonstrate that the miPSA has additional utility in assessing anti-miR cross-reactivity with miRNAs sharing similar seed sequences. PMID:26384419

  9. miR-203 and miR-221 regulate SOCS1 and SOCS3 in essential thrombocythemia

    PubMed Central

    Navarro, A; Pairet, S; Álvarez-Larrán, A; Pons, A; Ferrer, G; Longarón, R; Fernández-Rodríguez, C; Camacho, L; Monzó, M; Besses, C; Bellosillo, B

    2016-01-01

    The biological basis of essential thrombocythemia (ET) patients lacking known mutations is still unknown. MicroRNAs (miRNA) regulate hematopoietic differentiation and are deregulated in several hematopoietic malignancies. However, miRNA expression in ET patients has been poorly explored. We performed miRNA profiling in platelets from 19 ET patients and 10 healthy controls. Hierarchical cluster analysis showed two well-separated clusters between patients and controls, indicating that ET platelets had a characteristic 70-miRNA signature (P<0.0001), 68 of which were downregulated. According to the mutational status, three differentially expressed miRNAs, miR-15a (P=0.045), miR-150 (P=0.001) and miR-519a (P=0.036), were identified. A 40-miRNA signature was identified characterizing JAK2V617F-positive ET patients. Eight genes, whose interaction with the miRNAs could activate the JAK/STAT pathway were identified. An inverse correlation was observed between miRNAs expression and their target genes for SOCS1 and miR-221, SOCS3 and miR-221, SOCS3 and miR-203, and PTPN11 and miR-23a. All three miRNAs were upregulated in JAK2V617F-negative ET patients. SOCS1 and SOCS3 were validated as targets of miR-221 and miR-203, respectively. In summary, our study shows that platelets from JAK2V617F-negative ET patients harbor a specific miRNA signature that can participate in the modulation of the JAK/STAT pathway through regulation of key genes as SOCS1 and SOCS3. PMID:26990535

  10. Polysome shift assay for direct measurement of miRNA inhibition by anti-miRNA drugs.

    PubMed

    Androsavich, John R; Sobczynski, Daniel J; Liu, Xueqing; Pandya, Shweta; Kaimal, Vivek; Owen, Tate; Liu, Kai; MacKenna, Deidre A; Chau, B Nelson

    2016-01-29

    Anti-miRNA (anti-miR) oligonucleotide drugs are being developed to inhibit overactive miRNAs linked to disease. To help facilitate the transition from concept to clinic, new research tools are required. Here we report a novel method--miRNA Polysome Shift Assay (miPSA)--for direct measurement of miRNA engagement by anti-miR, which is more robust than conventional pharmacodynamics using downstream target gene derepression. The method takes advantage of size differences between active and inhibited miRNA complexes. Active miRNAs bind target mRNAs in high molecular weight polysome complexes, while inhibited miRNAs are sterically blocked by anti-miRs from forming this interaction. These two states can be assessed by fractionating tissue or cell lysates using differential ultracentrifugation through sucrose gradients. Accordingly, anti-miR treatment causes a specific shift of cognate miRNA from heavy to light density fractions. The magnitude of this shift is dose-responsive and maintains a linear relationship with downstream target gene derepression while providing a substantially higher dynamic window for aiding drug discovery. In contrast, we found that the commonly used 'RT-interference' approach, which assumes that inhibited miRNA is undetectable by RT-qPCR, can yield unreliable results that poorly reflect the binding stoichiometry of anti-miR to miRNA. We also demonstrate that the miPSA has additional utility in assessing anti-miR cross-reactivity with miRNAs sharing similar seed sequences. PMID:26384419