Science.gov

Sample records for howard pat dale

  1. Dale Sayers Festschrift

    SciTech Connect

    Stern, Edward A.

    2007-02-02

    This Festschrift is in honor of Dale Sayers who passed away in November 2004. Dale played a pivotal role in initiating the modern era of X-ray Absorption Fine Structure (XAFS) 35 years ago. The prehistory of XAFS before the modern era consisted of 40 years of confusion caused by Kronig's two different theories of the extended XAFS (EXAFS), the Short-Range Order (SRO) and Long-Range Order (LRO) theories. Dale's PhD thesis on EXAFS led to the idea of a Fourier transform to definitely prove that SRO is the correct theory and then to the development of XAFS as a structure determination technique.

  2. John Galen Howard.

    ERIC Educational Resources Information Center

    Draper, Joan

    1979-01-01

    A biographical sketch of John Galen Howard, founder of the Department of Architecture at the University of California at Berkeley, is presented. Howard's conservative outlook and idealistic nature are examined and his influence on the curriculum at the university is traced. (PHR)

  3. Reinventing Howard's Law School.

    ERIC Educational Resources Information Center

    Glenn, Gwendolyn

    2001-01-01

    Describes efforts by Alice Gresham Bullock, dean of the Howard University School of Law, to improve the school (including building a new library and improving relations with faculty) and restore Howard to its previous stature as a leader in civil rights law. (EV)

  4. Pat Mora.

    ERIC Educational Resources Information Center

    Corsaro, Julie

    1997-01-01

    Discusses Pat Mora's career as a children's book author and aspects of her work: the Latino experience, bilingualism, family life, desert landscape, and folk tales. Provides classroom activities in art, creative writing, social studies, geography, drama. Provides a selected bibliography of Mora's work. (PEN)

  5. The Howard Zinn Show

    ERIC Educational Resources Information Center

    Sewall, Gilbert T.

    2012-01-01

    Howard Zinn's "A People's History of the United States" is the nation's best-known work of American history. It is also the nation's best-selling survey of American history, having sold two million copies since its publication in 1980 and still selling about 125,000 paperback copies yearly. The fifth and current edition covers America up to the…

  6. Howard University Bookstore

    ERIC Educational Resources Information Center

    Maxon, Hazel Carter; Negron, Jaime

    1977-01-01

    Two full-time university bookstores, with three satellites helping during rush period, serve the Howard students and faculty. Solutions to problems of space, acquiring used books, and communications with faculty members are discussed, and the successful retailing of black studies books is described. (LBH)

  7. Howard Zinn on Democratic Education

    ERIC Educational Resources Information Center

    Zinn, Howard

    2004-01-01

    Perhaps no other historian has had a more profound and revolutionary impact on American education than Howard Zinn. This is the first book devoted to his views on education and its role in a democratic society. "Howard Zinn on Democratic Education" describes what is missing from school textbooks and in classrooms--and how we move beyond these…

  8. Molly Howard's School of HOPE

    ERIC Educational Resources Information Center

    Lile, Sarah J.

    2008-01-01

    The 2008 MetLife/NASSP High School Principal of the Year, Molly Howard, was working as a special education administrator in another part of the county when Jefferson County (GA) High School was being constructed in fall 1994. It was to be the only high school in the county, the consolidation of two high schools. In this article, the author…

  9. Profiles in Research: Howard Wainer

    ERIC Educational Resources Information Center

    Robinson, Dan

    2005-01-01

    Robinson interviews Howard Wainer, a scientist, who graduated from Rensselaer Polytechnic Institute in 1965 and Princeton in 1968 with degrees in mathematics and psychometrics, respectively. He taught at Temple University and The University of Chicago before moving to the Bureau of Social Science Research during the Carter Administration. Wainer…

  10. Implementing PAT with Standards

    NASA Astrophysics Data System (ADS)

    Chandramohan, Laakshmana Sabari; Doolla, Suryanarayana; Khaparde, S. A.

    2016-02-01

    Perform Achieve Trade (PAT) is a market-based incentive mechanism to promote energy efficiency. The purpose of this work is to address the challenges inherent to inconsistent representation of business processes, and interoperability issues in PAT like cap-and-trade mechanisms especially when scaled. Studies by various agencies have highlighted that as the mechanism evolves including more industrial sectors and industries in its ambit, implementation will become more challenging. This paper analyses the major needs of PAT (namely tracking, monitoring, auditing & verifying energy-saving reports, and providing technical support & guidance to stakeholders); and how the aforesaid reasons affect them. Though current technologies can handle these challenges to an extent, standardization activities for implementation have been scanty for PAT and this work attempts to evolve them. The inconsistent modification of business processes, rules, and procedures across stakeholders, and interoperability among heterogeneous systems are addressed. This paper proposes the adoption of specifically two standards into PAT, namely Business Process Model and Notation for maintaining consistency in business process modelling, and Common Information Model (IEC 61970, 61968, 62325 combined) for information exchange. Detailed architecture and organization of these adoptions are reported. The work can be used by PAT implementing agencies, stakeholders, and standardization bodies.

  11. Howard University Assembles Fund-Raising Juggernaut

    ERIC Educational Resources Information Center

    Masterson, Kathryn

    2008-01-01

    As a dental student 35 years ago, Leo E. Rouse and his Howard University classmates learned to fill cavities and cap teeth by crowding around one faculty member and angling for a clear view of the day's demonstration. Today students at Howard's College of Dentistry, where Dr. Rouse is now the dean, get an unobstructed view of dental procedures…

  12. Howard Zinn and the Socially Conscious Academic

    ERIC Educational Resources Information Center

    McKivigan, John R.

    2009-01-01

    In recent decades many people came to know Howard Zinn for his outspoken advocacy on a wide range of progressive causes, including civil rights, free speech, workers' rights, education reform, and opposition to U.S. imperialism. The author's own first encounter with Howard Zinn's special combination of scholarship and activism occurred several…

  13. Oral Histories in Meteoritics and Planetary Science - XX: Dale Cruikshank

    NASA Astrophysics Data System (ADS)

    Sears, Derek W. G.

    2013-04-01

    In this interview, Dale Cruikshank (Fig. 1) explains how as an undergraduate at Iowa State University he was a summer student at Yerkes Observatory where he assisted Gerard Kuiper in work on his Photographic Lunar Atlas. Upon completing his degree, Dale went to graduate school at the University of Arizona with Kuiper where he worked on the IR spectroscopy of the lunar surface. After an eventful 1968 trip to Moscow via Prague, during which the Soviets invaded Czechoslovakia, Dale assumed a postdoc position with Vasili Moroz at the Sternberg Astronomical Institute and more observational IR astronomy. Upon returning to the United States and after a year at Arizona, Dale assumed a position at the University of Hawai'i that he held for 17 years. During this period Dale worked with others on thermal infrared determinations of the albedos of small bodies beyond the asteroid Main Belt, leading to the recognition that low-albedo material is prevalent in the outer solar system that made the first report of complex organic solids on a planetary body (Saturn's satellite Iapetus). After moving to Ames Research Center, where he works currently, he continued this work and became involved in many outer solar system missions. Dale has served the community through his involvement in developing national policies for science-driven planetary exploration, being chair of the DPS 1990-1991 and secretary/treasurer for 1982-1985. He served as president of Commission 16 (Physics of Planets) of the IAU (2001-2003). He received the Kuiper prize in 2006.

  14. Dale Chihuly: An Inspiration in Art, Science, and Math!

    ERIC Educational Resources Information Center

    Hubbert, Beth

    2009-01-01

    Connecting students to the arts in a concrete way can be an effective teaching tool. In this article, the author describes how Dale Chihuly's "Hart Window," which features hand-blown glass disks affixed to the framework of the window, can be an inspiration for interdisciplinary connections in art, science and math. (Contains 4 online resources.)

  15. Aesthetics at the Impasse: The Unresolved Property of Dale Farm

    ERIC Educational Resources Information Center

    McCarthy, Lynne

    2015-01-01

    "Soil Depositions" was an art activist project that responded to the 2011 Irish Traveller eviction at Dale Farm in Essex when three resident women donated small amounts of soil from the site of their former home. The soil was subsequently deposited, framed and documented in various national and international locations. This article…

  16. 105. Photocopy of plate opposite page 105 in Robert Dale ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    105. Photocopy of plate opposite page 105 in Robert Dale Owen, Hints on Public Architecture (New York, G. P. Putnam, 1849). GROUND-PLANS, SMITHSONIAN INSTITUTION - Smithsonian Institution Building, 1000 Jefferson Drive, between Ninth & Twelfth Streets, Southwest, Washington, District of Columbia, DC

  17. Astronaut Dale Gardner holds up for sale sign after EVA

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Astronaut Dale A. Gardner, having just completed the major portion of his second extravehicular activity (EVA) period in three days, holds up a 'for sale' sign. Astronaut Joseph P. ALlen IV, who also participated in the two EVA's, is reflected in Gardner's helmet visor. A portion of each of two recovered satellites is in the lower right corner, with Westar nearer Discovery's aft.

  18. Howard Hughes and His Colorful Aircraft Career

    ERIC Educational Resources Information Center

    Karwatka, Dennis

    2012-01-01

    The HK-1 "Hercules" airplane made its maiden flight over 60 years ago, and it still holds the record as the airplane with the largest wingspan that ever flew. Powered by eight massive 28-cylinder engines, it was piloted by Howard Hughes during its one brief flight in California. A large portion of the airplane was made of wood, which gave rise to…

  19. Howard Zinn: Historian/Teacher as Citizen

    ERIC Educational Resources Information Center

    Jaramillo, Nathalia E.

    2009-01-01

    Few scholars have achieved the level of recognition and respect among the wider public as Howard Zinn. This should not come as a surprise, given the "unconventional" ways that Zinn embodied and enacted his scholarship. He often took his teaching and writing into those seemingly restricted spaces of popular protest, converting words into…

  20. Howard Community College 1998 Performance Accountability Report.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD. Office of Planning and Evaluation.

    This is a report on the 1998 performance accountability assessment conducted by Howard Community College (HCC) (Maryland) to examine overall institutional effectiveness. Enrollment trends show that more students are attending HCC, which will require more facility space, instructors, and student support. HCC addresses this issue by offering more…

  1. Howard Bowen: A Statesman of Higher Education.

    ERIC Educational Resources Information Center

    Stephens, Harrison

    1985-01-01

    Howard R. Bowen of The Claremont Graduate School has been president of three academic institutions. Because of his faculty and administrative experience in colleges and universities, he has a unique insight into how American higher education is governed and the future of higher education. (MLW)

  2. Howard Parnes, MD | Division of Cancer Prevention

    Cancer.gov

    Dr. Howard Parnes received a BA from Cornell University in 1977 and an MD from the University of Medicine and Dentistry of New Jersey in 1981. He trained in internal medicine at the Johns Hopkins Bayview Medical Center from 1981 to 1984 followed by a medical oncology fellowship at the University of Maryland Cancer Center (UMCC) from 1984 to 1987. |

  3. The scientific legacy of Howard Vincent Malmstadt

    NASA Astrophysics Data System (ADS)

    Horlick, Gary

    2006-06-01

    Howard Malmstadt was a true giant of Analytical Chemistry and clearly one of the most influential analytical chemists of the last 50 years. Howard, through his own work and that of his students (first generation) and their students (second generation) and their students' students (third generation) changed the course of Analytical Chemistry. His research interests were broad and ranged from analytical solution chemistry (titrimetry and reaction rates) and electrochemistry to atomic and molecular spectroscopy, chemical instrumentation, clinical chemistry and automation. Howard was also one of the most innovative and influential educators of our time. He changed forever the analytical curriculum through his many books on Electronics for Scientists, most written in conjunction with Chris Enke and Stan Crouch. Their texts and short courses went from pioneering the application of tube-based analog electronics (servo systems and operational amplifiers) in scientific measurements to the impact that integrated circuits and digital electronics would have on laboratory measurements. He strongly believed in the importance of "hands-on" in education. To this end, he expended considerable personal effort and time to see not only the development and commercialization of an effective laboratory infrastructure to support education in analog and digital electronics, but also oversaw the development of modular instrumentation for spectroscopy. Over the years he received many awards from the Analytical Chemistry community for his outstanding efforts and contributions to teaching and research. Many of Howard's students went on into academia. They and their students now represent the ongoing legacy for analytical chemistry that evolved from Howard's laboratory at Illinois. A remarkable diversity of research programs are underway in their laboratories. Topics range from atomic, laser, mass, and Raman spectroscopy to detection technology, analytical education, micro

  4. Howard University Engineers Success: Interdisciplinary Study Keeps Howard on the Cutting Edge

    ERIC Educational Resources Information Center

    Chew, Cassie M.

    2004-01-01

    According to Engineering Workforce Commission annual reports, in 1999 Howard University graduated 108 students, 92 of whom were African American, in its chemical, civil, electrical, and mechanical engineering programs and computer science programs. After two more years of graduating approximately 100 students across programs, in 2002, according to…

  5. Howard H. Kendler (1919-2011).

    PubMed

    Foley, John M

    2012-09-01

    Presents an obituary for Howard H. Kendler. Kendler was born in New York City on June 9, 1919, and died in Santa Barbara, California, on February 17, 2011. Kendler majored in psychology at Brooklyn College, where he was an assistant to Abraham Maslow, the father of humanistic psychology, and did a research project on the psychology of thinking under the supervision of Solomon Asch, a leading Gestalt psychologist. Howard Kendler's career was characterized by insightful experiments and a consistent analysis of the fundamental issues of the nature of our science, its methods, and its role in the community. The breadth of his knowledge and the depth of his thinking on these issues are rare. Although he did not settle these issues, he brought them into clear focus and forcefully advocated for his point of view. He was a visiting professor at the University of California, Berkeley, Hebrew University in Jerusalem, and Tel-Aviv University and was a fellow at the Center for Advanced Study in the Behavioral Sciences. He held the offices of president of the Western Psychological Association, chairman of the Board of Governors of the Psychonomic Society, and president of the Division of General Psychology and the Division of Experimental Psychology of the American Psychological Association. PMID:22963414

  6. Howard County Employer Survey, 1983-1984. Final Report.

    ERIC Educational Resources Information Center

    Radcliffe, Susan K.; Nespoli, Lawrence A.

    In summer 1983, the Howard County Employment Planning Council, Howard Community College, and several other county agencies and businesses joined forces in a countywide planning activity designed to identify important employment trends in the county and to define the need for training and retraining programs in response to those trends. A survey…

  7. Howard Beach Youth: A Study of Racial and Ethnic Attitudes.

    ERIC Educational Resources Information Center

    Lichter, Linda S.; Lichter, S. Robert

    This assessment of the climate of racial and ethnic attitudes in Howard Beach (New York) was conducted at John Adams High School, the public school attended by the greatest number of high school children in the Howard Beach community. The survey of 1,217 students was administered in December, 1986, several weeks before the incident in which a…

  8. James Newton Howard: JAMs with TRI-M.

    ERIC Educational Resources Information Center

    Reninger, Rosemary D.

    2000-01-01

    Presents an interview with James Newton Howard, a film composer. Provides background information on Howard. Addresses topics such as his most challenging and rewarding scores, his musical background, and the benefits of being associated with the American Society of Composers, Authors, and Publishers (ASCAP). (CMK)

  9. 29. Historic American Buildings Survey Photocopy of C. Howard Crane ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    29. Historic American Buildings Survey Photocopy of C. Howard Crane Office Drawing of 1919. Courtesy Smith, Hinchman & Grylls Associates, Inc., Detroit, 1973 ORNAMENTAL IRON DETAILS: POSTER BOARDS AND STORE FRONTS, ETC. - Orchestra Hall, 3711 Woodward Avenue, Detroit, MI

  10. 12. Historic American Buildings Survey Rendering by C. Howard Crane ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Historic American Buildings Survey Rendering by C. Howard Crane Office, 1919. Courtesy Detroit Historical Society. Photocopy by Jack E. Boucher, 1973 PERSPECTIVE VIEW FROM SOUTH - Orchestra Hall, 3711 Woodward Avenue, Detroit, MI

  11. 14. Historic American Buildings Survey Photocopy of C. Howard Crane ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Historic American Buildings Survey Photocopy of C. Howard Crane Office Drawing, 1919, Courtesy Smith, Hinchman & Grylls Associates, Inc., Detroit, 1973 PLOT PLAN (TITLE SHEET) - Orchestra Hall, 3711 Woodward Avenue, Detroit, MI

  12. Ecological risk assessment and sources of heavy metals in sediment from Daling River basin.

    PubMed

    Zhao, Lei; Mi, Dong; Chen, Yifu; Wang, Luo; Sun, Yeqing

    2015-04-01

    To investigate the distribution, source, and ecological risk of heavy metals in Daling River basin, 28 surface sediments collected in this region were analyzed by experimental and theoretical methods. Seven heavy metals, including Pb, Cr, Hg, Cu, As, Cd, and Zn, were detected in all samples. Monte Carlo simulation was used to assess the ecological risks of these heavy metals. It was found that the pollution of Cd was the most serious; the ecological risks in Daling River and Bohai Bay were significantly higher than those in estuary, Bohai Sea, and wetland, but overall, the ecological risks of these heavy metals were low to aquatic organisms in Daling River basin at present. Correlation analysis, principal component analysis, and cluster analysis showed that these heavy metals might originate from the same pollution sources located near Daling River and Bohai Bay. PMID:25378031

  13. Howard Brenner's Legacy for Biological Transport Processes

    NASA Astrophysics Data System (ADS)

    Nitsche, Johannes

    2014-11-01

    This talk discusses the manner in which Howard Brenner's theoretical contributions have had, and long will have, strong and direct impact on the understanding of transport processes occurring in biological systems. His early work on low Reynolds number resistance/mobility coefficients of arbitrarily shaped particles, and particles near walls and in pores, is an essential component of models of hindered diffusion through many types of membranes and tissues, and convective transport in microfluidic diagnostic systems. His seminal contributions to macrotransport (coarse-graining, homogenization) theory presaged the growing discipline of multiscale modeling. For biological systems they represent the key to infusing diffusion models of a wide variety of tissues with a sound basis in their microscopic structure and properties, often over a hierarchy of scales. Both scientific currents are illustrated within the concrete context of diffusion models of drug/chemical diffusion through the skin. This area of theory, which is key to transdermal drug development and risk assessment of chemical exposure, has benefitted very directly from Brenner's contributions. In this as in other areas, Brenner's physicochemical insight, mathematical virtuosity, drive for fully justified analysis free of ad hoc assumptions, quest for generality, and impeccable exposition, have consistently elevated the level of theoretical understanding and presentation. We close with anecdotes showing how his personal qualities and warmth helped to impart high standards of rigor to generations of grateful research students. Authors are Johannes M. Nitsche, Ludwig C. Nitsche and Gerald B. Kasting.

  14. Obituary: Howard H. Lanning, 1946-2007

    NASA Astrophysics Data System (ADS)

    Wade, Richard A.; MacConnell, D. Jack

    2009-01-01

    Howard H. Lanning died 20 December 2007 in Tucson, Arizona. He was a Software Quality Assurance Engineer for the Data Products Program at the National Optical Astronomy Observatory [NOAO] in Tucson, having returned to his native West after twenty years at the Space Telescope Science Institute [STScI] in Baltimore, Maryland. Throughout his career in roles supporting other astronomers, Howard steadily and persistently worked at his own research programs, most notably the identification and study of UV-bright sources in the Sandage Two-Color Survey of the Galactic Plane. Minor planet 2000 QJ248 has been named (61913) Lanning in recognition of his contributions. The son of James Clyde Lanning and Ethel Malan Lanning, Howard was born 26 May 1946, in Brawley, a small community near Calipatria, California. His parents worked for the local school district where his late father was Superintendent of Maintenance and Grounds and his mother was a school principal. After graduating from high school in a class of 52, Howard earned his A.A. degree at Imperial Valley Junior College, majoring in Astronomy and minoring in Mathematics. He completed his A.B. degree in Applied Arts and Sciences at San Diego State University [SDSU] in 1969 with the same major and minor. In 1974, he completed his M.S. in Astronomy at SDSU with a thesis on the period variation of the white-dwarf eclipsing binary BD +16∘516. During his time at SDSU Lanning was a Research Assistant/Observer at the former Hale Observatories, working on the optical identification of X-ray sources under the direction of Allan Sandage. His second refereed paper, the first of several finding lists of UV-bright stars arising from this work, appeared in 1973. Fellow students and faculty remember that he was a self-starter, initiating many observing projects of his own at Mount Laguna Observatory and encouraging others to do the same. For more than a decade Lanning was an observer and night assistant at Mount Wilson Observatory

  15. Obituary: Howard H. Lanning, 1946-2007

    NASA Astrophysics Data System (ADS)

    Wade, Richard A.; MacConnell, D. Jack

    2009-01-01

    Howard H. Lanning died 20 December 2007 in Tucson, Arizona. He was a Software Quality Assurance Engineer for the Data Products Program at the National Optical Astronomy Observatory [NOAO] in Tucson, having returned to his native West after twenty years at the Space Telescope Science Institute [STScI] in Baltimore, Maryland. Throughout his career in roles supporting other astronomers, Howard steadily and persistently worked at his own research programs, most notably the identification and study of UV-bright sources in the Sandage Two-Color Survey of the Galactic Plane. Minor planet 2000 QJ248 has been named (61913) Lanning in recognition of his contributions. The son of James Clyde Lanning and Ethel Malan Lanning, Howard was born 26 May 1946, in Brawley, a small community near Calipatria, California. His parents worked for the local school district where his late father was Superintendent of Maintenance and Grounds and his mother was a school principal. After graduating from high school in a class of 52, Howard earned his A.A. degree at Imperial Valley Junior College, majoring in Astronomy and minoring in Mathematics. He completed his A.B. degree in Applied Arts and Sciences at San Diego State University [SDSU] in 1969 with the same major and minor. In 1974, he completed his M.S. in Astronomy at SDSU with a thesis on the period variation of the white-dwarf eclipsing binary BD +16∘516. During his time at SDSU Lanning was a Research Assistant/Observer at the former Hale Observatories, working on the optical identification of X-ray sources under the direction of Allan Sandage. His second refereed paper, the first of several finding lists of UV-bright stars arising from this work, appeared in 1973. Fellow students and faculty remember that he was a self-starter, initiating many observing projects of his own at Mount Laguna Observatory and encouraging others to do the same. For more than a decade Lanning was an observer and night assistant at Mount Wilson Observatory

  16. Time-dependent Brittle Deformation in Darley Dale Sandstone

    NASA Astrophysics Data System (ADS)

    Baud, P.; Heap, M. J.; Meredith, P. G.; Bell, A. F.; Main, I. G.

    2008-12-01

    The characterization of time-dependent brittle rock deformation is fundamental to understanding the long- term evolution and dynamics of the Earth's upper crust. The chemical influence of water promotes time- dependent deformation through stress corrosion cracking that allows rocks to deform at stresses far below their short-term failure strength. Here we report results from a study of time-dependent brittle creep in water- saturated samples of Darley Dale sandstone (initial porosity of 13%). Conventional creep experiments (or 'static fatigue' tests) show that time to failure decreases dramatically with the imposed deviatoric stress. They also suggest the existence of a critical level of damage beyond which localized failure develops. Sample variability results however in significant scattering in the experimental data and numerous tests are needed to clearly define a relation between the strain rate and the applied stress. We show here that stress-stepping experiments provide a means to overcome this problem and that it is possible this way to obtain the strain rate dependence on applied stress with a single test. This allows to study in details the impact of various thermodynamical conditions on brittle creep. The influence of effective stress was investigated in stress-stepping experiments with effective confining pressures of 10, 30 and 50 MPa (whilst maintaining a constant pore fluid pressure of 20 MPa). In addition to the expected purely mechanical influence of an elevated effective stress our results also demonstrate that stress corrosion appears to be inhibited at higher effective stresses. The influence of doubling the pore fluid pressure however, whilst maintaining a constant effective stress, is shown to have no effect on the rate of stress corrosion. We then discuss the results in light of acoustic emission hypocentre location data and optical microscope analysis and use our experimental data to validate proposed macroscopic creep laws. Finally, using

  17. Dale Reed with model in front of M2-F1

    NASA Technical Reports Server (NTRS)

    1967-01-01

    Dale Reed with a model of the M2-F1 in front of the actual lifting body. Reed used the model to show the potential of the lifting bodies. He first flew it into tall grass to test stability and trim, then hand-launched it from buildings for longer flights. Finally, he towed the lifting-body model aloft using a powered model airplane known as the 'Mothership.' A timer released the model and it glided to a landing. Dale's wife Donna used a 9 mm. camera to film the flights of the model. Its stability as it glided--despite its lack of wings--convinced Milt Thompson and some Flight Research Center engineers including the center director, Paul Bikle, that a piloted lifting body was possible. The lifting body concept evolved in the mid-1950s as researchers considered alternatives to ballistic reentries of piloted space capsules. The designs for hypersonic, wingless vehicles were on the boards at NASA Ames and NASA Langley facilities, while the US Air Force was gearing up for its Dyna-Soar program, which defined the need for a spacecraft that would land like an airplane. Despite favorable research on lifting bodies, there was little support for a flight program. Dryden engineer R. Dale Reed was intrigued with the lifting body concept, and reasoned that some sort of flight demonstration was needed before wingless aircraft could be taken seriously. In February 1962, he built a model lifting body based upon the Ames M2 design, and air-launched it from a radio controlled 'mothership.' Home movies of these flights, plus the support of research pilot Milt Thompson, helped pursuade the facilities director, Paul Bikle, to give the go-ahead for the construction of a full-scale version, to be used as a wind-tunnel model and possibly flown as a glider. Comparing lifting bodies to space capsules, an unofficial motto of the project was, 'Don't be Rescued from Outer Space--Fly Back in Style.' The construction of the M2-F1 was a joint effort by Dryden and a local glider manufacturer, the

  18. The correspondence of Thomas Dale (1700-1750): Botany in the transatlantic Republic of Letters.

    PubMed

    Cook, William J

    2012-03-01

    This paper seeks to provide a full account of the life and career of Dr. Thomas Dale (1700-1750), with particular reference to his botanical works and correspondence. Born in Hoxton, London, Dale studied medicine at Leiden and engaged fully in the social, literary and epistolary network in which botany was practised in eighteenth-century England. In 1730, however, Dale relocated to the British colonial port of Charles Town, South Carolina. Here he continued to engage in a transatlantic network of botanical exchange and discussion, corresponding on equal and reciprocal terms with his former colleagues in England. Where Dale differs from naturalists in South Carolina before him is that his motives for pursuing botany and for corresponding with English naturalists were located firmly in the New World. Such a conclusion forms a valuable, albeit small contribution to models for the development of national scientific cultures in the imperial world. Similarly, Dale's pursuit of botanical information in South Carolina provides a small amount of material with which to illustrate currently fashionable models for the mediated exchange and circulation of scientific knowledge. PMID:22326093

  19. Mr Howard Goes to Washington--and Brings Home Victory

    ERIC Educational Resources Information Center

    Glover, Dennis

    2004-01-01

    In June 2002--seven months after the most divisive federal election campaign since 1975--Mr. John Howard went to Washington to be feted by the International Democratic Union--the worldwide association of centre-right political parties. So impressed were they by his against-the-odds victory that they elected him as their Chairman. In a White House…

  20. Undue Certainty: Where Howard Zinn's "A People's History" Falls Short

    ERIC Educational Resources Information Center

    Wineburg, Sam

    2013-01-01

    Howard Zinn's "A People's History" of the United States has few peers among contemporary historical works. With more than 2 million copies in print, "A People's History" is more than a book. It is a cultural icon. While most historians aim to examine the full historical record, Zinn picks and chooses from it. Writing persuasively, he hides the…

  1. HOWARD UNIVERSITY STUDENTS AND THE COMMUNITY SERVICE PROJECT.

    ERIC Educational Resources Information Center

    LOWE, GILBERT A., JR.

    THIS REPORT DESCRIBES THE INVOLVEMENT OF HOWARD UNIVERSITY STUDENTS IN A FIVE-YEAR COMMUNITY SERVICE PROJECT IN THE SECOND PRECINCT IN WASHINGTON, D.C., BETWEEN 1961 AND 1966. THE PROJECT WAS PLANNED TO--(1) MOBILIZE AND ASSIST THE MORE THAN 160 CHURCHES IN THE AREA, (2) SURVEY THE NEEDS OF THE RESIDENTS AND HELP THEM SOLVE THEIR PROBLEMS, (3)…

  2. Rise of Human Intelligence: Comments on Howard (1999).

    ERIC Educational Resources Information Center

    Gobet, Fernand; Campitelli, Guillermo; Waters, Andrew J.

    2002-01-01

    Finds several difficulties with the theory advanced by R. Howard and shows that alternative explanations relating to changes in the chess environment, including increased access to chess knowledge, offer better explanations for the increased presence of young players at top-level chess. (SLD)

  3. Evaluation of the Howard Hughes Science Grant Project, Year One

    ERIC Educational Resources Information Center

    Wolanin, Natalie; Wade, Julie

    2015-01-01

    The goal of the Howard Hughes Science Institute (HHMI) supported science program is to train one staff member to become a science lead within each of the elementary schools in the Montgomery County (Maryland) Public Schools (MCPS) district. The specific objectives of the first year of HHMI grant project were to: (1) provide approximately 20…

  4. Howard Bowen Revisited: What's Happened to Academic Compensation.

    ERIC Educational Resources Information Center

    Kacmarczyk, Ronald H.; Coughlin, Donna M.

    1984-01-01

    Trends in the monetary and non-monetary compensation of academic personnel were compared to data on compensation in government, business, and industry, and the non-academic not-for-profit sector. Findings from 1981-82 were compared to Howard R. Bowen's findings for 1976-77. (MLW)

  5. A Review of Howard University's Financial Literacy Curriculum

    ERIC Educational Resources Information Center

    Lindsey-Taliefero, Debby; Kelly, Lynne; Brent, William; Price, Russell

    2011-01-01

    This article evaluates a financial literacy curriculum at the Howard University (HU) School of Business, by measuring the financial knowledge acquired after participating in a variety of programs. To evaluate the HU curriculum, the National Jump$tart Coalition (NJC) survey was administered to collect data on financial knowledge and demographic…

  6. A Poetry Workshop in Print: Pat Mora

    ERIC Educational Resources Information Center

    Hopkins, Lee Bennett

    2006-01-01

    After a successful career as a writer for adults, Pat Mora began creating books for children. Her first picture book, "Tomas and The Library Lady" (Knopf, 1997) is a tender story of a young migrant worker who unearths new worlds when he discovers the magic a public library holds. The text, cleverly interspersed with foreign words, became a…

  7. Antimatter/HiPAT Support Services

    NASA Technical Reports Server (NTRS)

    Lewis, Raymond A.

    2001-01-01

    Techniques were developed for trapping normal matter in the High Performance Antiproton Trap (HiPAT). Situations encountered included discharge phenomena, charge exchange and radial diffusion processes. It is important to identify these problems, since they will also limit the performance in trapping antimatter next year.

  8. HOWARD FORK ACID ROCK DRAINAGE SOURCE INTERCEPTION STUDY; HOWARD FORK OF THE SAN MIGUEL RIVER NEAR OPHIR, COLORADO

    EPA Science Inventory

    This project proposes to analyze regional hydrogeology as it relates to mine workings which discharge significant heavy metals into the Howard Fork of the San Miguel River and recommend strategies to intercept and divert water away from mineralized zones. The study also includes...

  9. The Relationship of Media and ISD Theory: The Unrealized Promise of Dale's Cone of Experience.

    ERIC Educational Resources Information Center

    Seels, Barbara

    Instructional systems design (ISD) is the integration of general systems theory, instructional theory, and communications theory. Edgar Dale, a leader in the fields of reading and journalism and a pioneer in the humanistic/communications tradition of the field of instructional technology, related the concrete to abstract continuum to media…

  10. Previous Attempts to Debunk the Mythical Retention Chart and Corrupted Dale's Cone

    ERIC Educational Resources Information Center

    Subramony, Deepak Prem; Molenda, Michael; Betrus, Anthony K.; Thalheimer, Will

    2014-01-01

    Critics have been attempting to debunk the mythical retention chart at least since 1971. The earliest critics, David Curl and Frank Dwyer, were addressing just the retention data. Beginning around 2002, a new generation of critics has taken on the illegitimate combination of the retention chart and Edgar Dale's Cone of Experience--the corrupted…

  11. Astronat Dale Gardner using MMU to travel to Westar VI satellite

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Astronat Dale A. Gardner appears to be under the remote manipulator system (RMS) end effector as he makes a turn in space and preapares to use the manned maneuvering unit (MMU) to travel to Westar VI satellite. The 'stinger' he carries will enter the communications satellite through the nozzle of the spent motor.

  12. Astronaut Dale Gardner using MMU to travel to Westar VI satellite

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Astronaut Dale A. Gardner, wearing the manned maneuvering unit (MMU) approaching the spinng Westar VI satellite over Bahama Banks. Gardner uses a large tool called the apogee kick motor capture device (ACD) to enter the nozzle of the spent Westar engine and stabilize the satellite to capture it for return to Earth.

  13. Astronaut Dale Gardner using MMU to travel to Westar VI satellite

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Astronaut Dale A. Gardner, wearing the manned maneuvering unit (MMU) approaching the spinng Westar VI satellite over Bahama Banks. The end effector of the remote manipulator system (RMS) controlled by Dr. Anna L. Fisher inside Discovery's cabin, awaits its duty at right.

  14. The Mythical Retention Chart and the Corruption of Dale's Cone of Experience

    ERIC Educational Resources Information Center

    Subramony, Deepak Prem; Molenda, Michael; Betrus, Anthony K.; Thalheimer, Will

    2014-01-01

    In response to the wide-scale proliferation of "the cone of learning"--a fanciful retention chart confounded with Dale's Cone of Experience--the authors make four major claims debunking this fantasy and provide documentary evidence to support these claims. The first claim is that the data in the mythical retention chart do not make…

  15. The evaluation of Pat-Pat related injuries in the western black sea region of Turkey

    PubMed Central

    2011-01-01

    Background Accidents caused by motorized vehicle in the agricultural sector are frequently observed. In Turkey; accidents arising from motorized vehicles, named Pat-Pat, which are used by farmers in the Western Black Sea region is not unusual. Methods One hundred five patients who were brought into the Emergency Department of Duzce University, Medical Faculty Hospital between September 2009 and August 2010 due to Pat-Pat related accidents were evaluated. Results The cases consisted of 73 (69.5%) males and 32 (30.5%) females, ranging from 2 to 73 years of age. In the 10-39 age group, a total of 63 (60.0%) cases were determined. The months when the greatest rate of cases applied to the hospital consisted of July, August, September and the season is summer. The cases were exposed to trauma in roads in 54 (51.4%), and 51 (48.6%) occurred in agricultural area without roads. Eighty seven (82.9%) cases were injured due to the overturning of vehicle. The patients were brought to the hospital using a private vehicle in 54 (51.4%) of the cases and in 51 (48.6%) cases, 112 ambulance system was used. The cases were determined to apply to the hospital most frequently between 6 pm-12 am. The injuries frequently consisted of head-neck and spine traumas, thorax traumas and upper extremity traumas. In 55 (52.4%) cases, open wound-laceration was determined. Seventy five (71.4%) cases were treated in the Emergency Department, and 28 (26.7%) were hospitalized. Three (2.9%) cases were deceased. Conclusions Serious injuries can occur in Pat-Pat related accidents, and careful systematic physical examination should be conducted. In order to prevent these accidents, education of farm operators and engineering studies on the mechanics and safety of these vehicles should be taken and legal regulations should be created. PMID:21699689

  16. Foundations of educational psychology: Howard Gardner's neoclassical psyche.

    PubMed

    Diessner, R

    2001-12-01

    This article is a theoretical examination of the implications of Howard Gardner's work in developmental and educational psychology (1983, 1993, 1999a, 1999b) for the structure of the psyche. The author accepts as axiomatic, in the context of this article, Gardner's educational manifesto (1999a) that all students should be taught disciplinary understandings of truth, beauty, and goodness. Rational inferences are then made indicating that the psyche that Gardner intends to educate and help develop is in the form of a neoclassical psyche and that it is structured by the capacities to know truth, to love beauty, and to will goodness. PMID:11831356

  17. PAT-1 safety analysis report addendum.

    SciTech Connect

    Weiner, Ruth F.; Schmale, David T.; Kalan, Robert J.; Akin, Lili A.; Miller, David Russell; Knorovsky, Gerald Albert; Yoshimura, Richard Hiroyuki; Lopez, Carlos; Harding, David Cameron; Jones, Perry L.; Morrow, Charles W.

    2010-09-01

    The Plutonium Air Transportable Package, Model PAT-1, is certified under Title 10, Code of Federal Regulations Part 71 by the U.S. Nuclear Regulatory Commission (NRC) per Certificate of Compliance (CoC) USA/0361B(U)F-96 (currently Revision 9). The purpose of this SAR Addendum is to incorporate plutonium (Pu) metal as a new payload for the PAT-1 package. The Pu metal is packed in an inner container (designated the T-Ampoule) that replaces the PC-1 inner container. The documentation and results from analysis contained in this addendum demonstrate that the replacement of the PC-1 and associated packaging material with the T-Ampoule and associated packaging with the addition of the plutonium metal content are not significant with respect to the design, operating characteristics, or safe performance of the containment system and prevention of criticality when the package is subjected to the tests specified in 10 CFR 71.71, 71.73 and 71.74.

  18. Howard Zonana and the transformation of forensic psychiatry.

    PubMed

    Bonnie, Richard J

    2010-01-01

    In recognition of Howard Zonana's contributions, I take stock of the progress of the field of forensic psychiatry over three decades. As forensic psychiatrists, you are the voice of psychiatry in the law and the interpreter of law to your colleagues in psychiatry. I offer provisional impressions of your collective accomplishments under three themes: expertise, influence, and integrity. First, you have established and nurtured the expertise and authority of forensic psychiatry, which has become increasingly evidence-based and reflects a prudent sense of humility. Second, you have had a significant influence on the design and daily application of mental health law in both criminal and civil spheres. Through the American Academy of Psychiatry and the Law (AAPL) and relevant components of the American Psychological Association, you have been a strong and effective advocate for protecting both the needs and rights of people with mental illness and have had demonstrable influence in the Supreme Court as well as at the grassroots. Finally, you have nurtured and protected the ethical integrity of the profession in the face of ever-increasing challenges, due in no small measure to the habits of ethical reflection that Howard Zonana has modeled and taught over the course of his extraordinary career. PMID:21156919

  19. The NOAA Center in Atmospheric Sciences (NCAS) at Howard University

    NASA Astrophysics Data System (ADS)

    Strachan, M. D.; Morris, V. R.

    2003-12-01

    The National Oceanic and Atmospheric Administration (NOAA) of the Department of Commerce established the NOAA Center for Atmospheric Sciences (NCAS), a Cooperative Science Center, in fall 2001 to support the development of quality education to students at minority serving institutions while meeting the prescribed goals of NOAA and the nation. NCAS was established to research some of the critical environmental conditions occurring nationally and globally, and to provide opportunities and programs for students to pursue careers in atmospheric, environmental, and oceanic sciences and remote sensing. A primary goal is to increase the number of highly qualified, well trained graduates in the fields of NOAA related atmospheric sciences. NCAS is led by Howard University, in collaboration with three partners - Jackson State University, the University of Texas at El Paso, and the University of Puerto Rico at Mayaguez. This presentation will highlight the activities and accomplishments in research, education, and outreach of NCAS over its first two years of existence. The primary benefactor of NCAS has been the Howard University Program in Atmospheric Sciences (HUPAS), a comprehensive graduate program in atmospheric sciences with core focus areas of atmospheric chemistry, atmospheric physics, and geophysical fluid dynamics.

  20. Analysis of Employee Supply and Demand in Howard County--A Pilot Approach.

    ERIC Educational Resources Information Center

    Howard Coll. at Big Spring, TX.

    A survey developed by the Occupational Education Division of Howard University to determine employee supply and demand in Howard County, Texas, involved both employees and employers. Employer data were compiled from interviews with 319 out of a possible 409 employers who employed three or more people. Conclusions and recommendations for the…

  1. Howard Thurman, Black Spirituality, and Critical Race Theory in Higher Education

    ERIC Educational Resources Information Center

    Giles, Mark S.

    2010-01-01

    This study examines aspects of Dr. Howard W Thurman's (1900-1982) career in higher education through the lenses of Black spirituality and critical race theory. The experiences of Howard Thurman offers distinct perspectives through which to interrogate the Black experience in American higher education and the intersections of race, religion and…

  2. Differential expression of proton-assisted amino acid transporters (PAT[1] and PAT[2]) in tissues of neonatal pigs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The PATs have been identified as growth-regulatory nutrient sensors in Drosophila and as activators of mammalian target of rapamycin (mTOR) in mammalian cell cultures. These studies suggest that, beyond their classical function as transporters of simple amino acids (AA), the PATs act as tranceptors,...

  3. Extension of the Gladstone-Dale equation for flame flow field diagnosis by optical computerized tomography

    SciTech Connect

    Chen Yunyun; Li Zhenhua; Song Yang; He Anzhi

    2009-05-01

    An extended model of the original Gladstone-Dale (G-D) equation is proposed for optical computerized tomography (OCT) diagnosis of flame flow fields. For the purpose of verifying the newly established model, propane combustion is used as a practical example for experiment, and moire deflection tomography is introduced with the probe wavelength 808 nm. The results indicate that the temperature based on the extended model is more accurate than that based on the original G-D equation. In a word, the extended model can be suitable for all kinds of flame flow fields whatever the components, temperature, and ionization are.

  4. The Planetary and Eclipse Oil Paintings of Howard Russell Butler

    NASA Astrophysics Data System (ADS)

    Pasachoff, Jay M.; Olson, R. M.

    2013-10-01

    The physics-trained artist Howard Russell Butler (1856-1934) has inspired many astronomy students through his planetary and eclipse paintings that were long displayed at the Hayden Planetarium in New York, the Fels Planetarium at the Franklin Institute in Philadelphia, and the Buffalo Museum of Science. We discuss not only the eclipse triptychs (1918, 1923, and 1925) at each of those institutions but also his paintings of Mars as seen from Phobos and from Deimos (with landscapes of those moons in the foreground depicted in additional oils hung at Princeton University) and the Earth from our Moon. We also describe his involvement with astronomy and his unique methodology that allowed him to surpass the effects then obtainable with photography, as well as his inclusion in a U.S. Naval Observatory eclipse expedition in 1918, as well as his auroral, solar-prominence, and 1932-eclipse paintings.

  5. Becoming respectable: T. Dale Stewart and the acceptance of forensic anthropology in the academic community.

    PubMed

    Kennedy, K A

    2000-03-01

    Before World War II, forensic anthropology was of peripheral interest to a few anthropologists willing to assist in investigations by law enforcement agencies. A strong bias that "police work" was unbecoming to the scholarly pursuits of academics persisted into the post-war years. Changes took place as a consequence of T. Dale Stewart's case work in the identification of human remains with the FBI from 1943 to 1969, his directorship of the National Museum of Natural History (Smithsonian Institution) beginning in 1962, and his work with the Armed Forces after 1948. This paper discusses the historic period of transition of attitudes and practices in the contexts of Stewart's contributions and the cases and teaching programs of one of his contemporaries. Theodore D. McCown at the University of California at Berkeley, during the period of 1939 to 1969. The establishment of the Physical Anthropology Section within the American Academy of Forensic Sciences in 1972 and the creation of the T. Dale Stewart award for distinguished service in forensic anthropology advanced those laboratory research programs and medical-legal investigations conducted by present-day forensic anthropologists. PMID:10782943

  6. Pat Thiel talks about Nobel Prize winner Dan Shechtman

    SciTech Connect

    Thiel, Pat

    2012-01-01

    Ames Laboratory senior scientist and Iowa State University Distinguished Professor of Chemistry Pat Thiel talks about her friend and colleague Dan Shechtman who received the 2011 Nobel Prize for Chemistry.

  7. Pat Thiel talks about Nobel Prize winner Dan Shechtman

    ScienceCinema

    Thiel, Pat

    2013-03-01

    Ames Laboratory senior scientist and Iowa State University Distinguished Professor of Chemistry Pat Thiel talks about her friend and colleague Dan Shechtman who received the 2011 Nobel Prize for Chemistry.

  8. Cosmopolitanism and Our Descriptions of Ethics and Ontology: A Response to Dale Snauwaert's "The Ethics and Ontology of Cosmopolitanism"

    ERIC Educational Resources Information Center

    Hansen, David T.

    2010-01-01

    In recent years scholars across the humanities and social sciences have revitalized the ancient concept of cosmopolitanism. Dale Snauwaert illuminates why this is so in his thoughtful article on what it might mean to educate for a shared humanity. Snauwaert shows why many people find so-called "realism" an unsatisfactory political and moral…

  9. Howard Russell Butler's Oil Paintings of Solar Eclipses and Prominences

    NASA Astrophysics Data System (ADS)

    Pasachoff, Jay M.; Olson, Roberta J. M.

    2014-06-01

    Howard Russell Butler (1856-1934) was invited to join the US Naval Observatory expedition to the total solar eclipse of 1918 because of his ability to paint astronomical phenomena based on quickly-made notes about spatial and color details. His giant triptych of the total eclipses of 1918, 1923, and 1925 was proposed for a never-built astronomical center at the American Museum of Natural History and wound up at their Hayden Planetarium when it was constructed in the mid-1930s. Half-size versions are installed at the Fels Planetarium at the Franklin Institute in Philadelphia and at the Firestone Library of Princeton University, whose newly conserved canvases were recently hung; the Buffalo Museum of Science has another half-size version in storage. We discuss not only the eclipse triptychs but also the series of large oil paintings he made of solar prominences (in storage at the American Museum of Natural History) and of his 1932-eclipse and other relevant works.JMP was supported for this work in part by Division III Discretionary Funds and the Brandi Fund of Williams College. His current eclipse research is supported by grants AGS-1047726 from the Solar Research Program of the Atmospheric and Geospace Sciences Division of NSF and 9327-13 from the Committee for Research and Exploration of the National Geographic Society.

  10. The Solar Eclipse Mural Series by Howard Russell Butler

    NASA Astrophysics Data System (ADS)

    Pasachoff, J. M.; Olson, R. J. M.

    2016-01-01

    There is a rich trove of astronomical phenomena in works of art by artists from the greater New York area, a trend that is even more pronounced in the oeuvres of New York City residents through the present day. A case in point is the trio of oil paintings by artist (and former physics professor) Howard Russell Butler depicting total solar eclipses in 1918, 1923, and 1925 that are based on his own observations. They were long displayed in the former art-deco building of the Hayden Planetarium of the American Museum of Natural History, the location of this conference. (The Museum also has nine other Butler paintings, none of which are currently exhibited.) Since the eclipse paintings have been in storage for many years, these once famous works are now virtually forgotten. Based on our research as an astronomer who has seen sixty-two solar eclipses and an art historian who has written extensively about astronomical imagery, we will discuss Butler's Solar Eclipse Triptych to explore its place in the history of astronomical imaging.

  11. Howard University Energy Expert Systems Institute Summer Program (EESI)

    NASA Technical Reports Server (NTRS)

    Momoh, James A.; Chuku, Arunsi; Abban, Joseph

    1996-01-01

    Howard University, under the auspices of the Center for Energy Systems and Controls runs the Energy Expert Systems Institute (EESI) summer outreach program for high school/pre-college minority students. The main objectives are to introduce precollege minority students to research in the power industry using modern state-of-the-art technology such as Expert Systems, Fuzzy Logic and Artificial Neural Networks; to involve minority students in space power management, systems and failure diagnosis; to generate interest in career options in electrical engineering; and to experience problem-solving in a teamwork environment consisting of faculty, senior research associates and graduate students. For five weeks the students are exposed not only to the exciting experience of college life, but also to the inspiring field of engineering, especially electrical engineering. The program consists of lectures in the fundamentals of engineering, mathematics, communication skills and computer skills. The projects are divided into mini and major. Topics for the 1995 mini projects were Expert Systems for the Electric Bus and Breast Cancer Detection. Topics on the major projects include Hybrid Electric Vehicle, Solar Dynamics and Distribution Automation. On the final day, designated as 'EESI Day' the students did oral presentations of their projects and prizes were awarded to the best group. The program began in the summer of 1993. The reaction from the students has been very positive. The program also arranges field trips to special places of interest such as the NASA Goddard Space Center.

  12. Multivariate PAT solutions for biopharmaceutical cultivation: current progress and limitations.

    PubMed

    Mercier, Sarah M; Diepenbroek, Bas; Wijffels, Rene H; Streefland, Mathieu

    2014-06-01

    Increasingly elaborate and voluminous datasets are generated by the (bio)pharmaceutical industry and are a major challenge for application of PAT and QbD principles. Multivariate data analysis (MVDA) is required to delineate relevant process information from large multi-factorial and multi-collinear datasets. Here the key role of MVDA for industrial (bio)process data is discussed, with a focus on progress and limitations of MVDA as a PAT solution for biopharmaceutical cultivation processes. MVDA based models were proven useful and should be routinely implemented for bioprocesses. It is concluded that although the highest level of PAT with process control within its design space in real-time during manufacturing is not reached yet, MVDA will be central to reach this ultimate objective for cell cultivations. PMID:24732022

  13. Rapid Response to the Howard Hanson Dam Crisis

    NASA Astrophysics Data System (ADS)

    Ralph, F. M.; Carter, G.; White, A.; Neiman, P. J.; King, C.; Jankov, I.; Colman, B.; Cook, K.; Buehner, T.

    2010-12-01

    Dedicated in 1962, the Howard A. Hanson Dam (HHD) brought necessary flood relief to the Green River Valley in the Metropolitan Area of Seattle, Washington, and opened the way for increased valley development. For example, the flood damage prevented by HHD during the extreme precipitation event in early January 2009 is estimated to be about $4 billion. However, following the record high level of water behind HHD caused by this event, the U.S. Army Corps of Engineers (ACE) became concerned about the Dam’s safety. Despite short-term measures to improve HHD during 2009, the chance for a significant Green River Valley flood event was estimated by the ACE to be 1 in 25 for the 2009/10 winter season. In response to this elevated risk, NOAA organized a coordinated effort across research and forecast operations to implement new observations, modeling and dissemination tools, and knowledge of the role of atmospheric rivers (ARs) in producing extreme precipitation, prototyped in California within NOAA’s Hydrometeorology Testbed (HMT; hmt.noaa.gov). Fortunately, although seasonal observed precipitation (October 2009 through March 2010) in the Seattle area was slightly greater than normal (e.g., 112% of normal at the Seattle National Weather Service Forecast Office), there were no threatening floods observed along the Green River. This outcome was influenced by a synoptic pattern that was progressive, i.e., storms did not stall over this vulnerable watershed, which reduced the chance for an extensive (time and space) extreme event. More than a decade of West Coast winter storm research conducted primarily in California by NOAA’s Earth System Research Laboratory/Physical Sciences Division (ESRL/PSD) has identified atmospheric rivers (ARs), narrow regions of enhanced water vapor transport, as the culprits that cause extreme precipitation events, such as the January 2009 event that stressed HHD. ESRL/PSD extended this AR research to the coast of Washington by deploying a

  14. Stimulant-Related Reductions of Growth Rates in the PATS

    ERIC Educational Resources Information Center

    Swanson, James; Greenhill, Laurence; Wigal, Tim; Kollins, Scott; Stehli, Annamarie; Davies, Mark; Chuang, Shirley; Vitiello, Benedetto; Skrobala, Anne; Posner, Kelly; Abikoff, Howard; Oatis, Melvin; McCracken, James; McGough, James; Riddle, Mark; Ghuman, Jaswinder; Cunningham, Charles; Wigal, Sharon

    2006-01-01

    Objective: To investigate growth of children with attention-deficit/hyperactivity disorder (ADHD) in the Preschool ADHD Treatment Study (PATS) before and after initiation of treatment with methylphenidate at titrated doses (average, 14.2 mg/day) administered three times daily, 7 days/week for approximately equal to 1 year. Method: The heights and…

  15. Pat Thiel talks about attending the Nobel Prize Award Ceremony

    ScienceCinema

    Thiel, Pat

    2013-03-01

    Pat Thiel, Ames Laboratory senior scientist and Iowa State University Distinguished Professor of Chemistry, was invited to be a guest at the ceremony on December 10th, in Stockholm, Sweden, where Danny Shechtman, Ames Laboratory scientist, received the 2011 Nobel Prize in Chemistry. Following her return to the Lab, Thiel shared some of her recollections of the momentous event.

  16. Pat Mora, Fiction/Nonfiction Writer and Poet (Profile).

    ERIC Educational Resources Information Center

    Barrera, Rosalinda B.

    1998-01-01

    Offers a profile of Pat Mora, a writer whose work crosses the boundaries of audience, genre, and language. Notes that woven through her works are topics drawn from her life experience. Looks at her recent accomplishments and forthcoming books. Discusses her advocacy for cultural and linguistic diversity, and for more Latinos in all phases of…

  17. Stable phantoms for characterization of photoacoustic tomography (PAT) systems

    NASA Astrophysics Data System (ADS)

    Bohndiek, Sarah E.; Van de Sompel, Dominique; Bodapati, Sandhya; Kothapalli, Sri Rajasekhar; Gambhir, Sanjiv S.

    2013-02-01

    Photoacoustic tomography (PAT) is an emerging modality that combines the high contrast of optical imaging, with the spatial resolution and penetration depth of ultrasound, by exploiting the photoacoustic effect. As with any new imaging modality, reliable physical phantoms are needed to: calibrate instruments; validate performance; optimize signal-to-noise; perform routine quality control; and compare systems. Phantom materials for testing small animal PAT systems should also mimic both the optical and acoustic properties of soft tissue, while for calibration purposes should be resistant to degradation over long time periods. We show here that polyvinyl chloride plastisol (PVCP) phantoms enable calibration and performance validation using two PAT systems with distinct designs (Visualsonics Vevo LAZR and Endra Nexus 128) across a wavelength range of 680 nm - 950 nm. Inclusions between 2 and 3.2 mm in diameter were fabricated from PVCP using a range of dye concentrations (0 % to 0.256 % Black Plastic Color, BPC) in a custom mold. A calibration phantom was imaged repeatedly on both systems, over time scales of minutes, hours and days, to assess system stability. Both systems demonstrated good reproducibility over time, with the coefficient of variation in the measured signal-to-noise ratio (SNR) being less than 15% over the course of 30 days. Imaging performance was optimized by plotting SNR as a function of different system parameters. The visualization of objects embedded in optically absorbing and scattering backgrounds was also assessed. PVCP is easy to work with and provides stable phantoms for assessing PAT system performance.

  18. Pat Thiel talks about attending the Nobel Prize Award Ceremony

    SciTech Connect

    Thiel, Pat

    2012-01-01

    Pat Thiel, Ames Laboratory senior scientist and Iowa State University Distinguished Professor of Chemistry, was invited to be a guest at the ceremony on December 10th, in Stockholm, Sweden, where Danny Shechtman, Ames Laboratory scientist, received the 2011 Nobel Prize in Chemistry. Following her return to the Lab, Thiel shared some of her recollections of the momentous event.

  19. The PATS Project: A State of Tennessee School Reform Initiative.

    ERIC Educational Resources Information Center

    Butler, E. Dean; And Others

    The first-year results of a Tennessee college-school partnership program to implement a data-based school improvement change model--the Positive Attitudes in Tennessee Schools Project (PATS)--are summarized in this report. Two major goals of the first year (1989-90) included assessing and improving school climate and enhancing student and teacher…

  20. 10. GLASS, SCHNEIDER & REZNER BRIDGE PATENT MODEL, PAT. NO. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. GLASS, SCHNEIDER & REZNER BRIDGE PATENT MODEL, PAT. NO. 71, 868, DECEMBER 10, 1867. THIS MODEL IS ONE OF A FEW THAT SURVIVED THE PATENT OFFICE FIRE OF 1877. IF REPRODUCED, CREDIT SHOULD BE GIVEN TO THE SMITHSONIAN INSTITUTION, NEGATIVE NO. 48660-D. - White Bowstring Arch Truss Bridge, Spanning Yellow Creek at Cemetery Drive (Riverside Drive), Poland, Mahoning County, OH

  1. Project Achieve Transition Services (PATS), 1993-94. OER Report.

    ERIC Educational Resources Information Center

    Goldberg, Phyllis

    Project Achieve Transition Services (PATS) is a four-year high school attendance improvement, dropout prevention project which targets late-entry students consisting primarily of immigrants, transfers, and long-term absentees. The program uses a case management approach to provide support services, instructional enhancements, and family outreach…

  2. Dynamic recrystallization mechanisms and their transition in the Daling Thrust (DT) zone, Darjeeling-Sikkim Himalaya

    NASA Astrophysics Data System (ADS)

    Ghosh, Subhajit; Bose, Santanu; Mandal, Nibir; Dasgupta, Sujoy

    2016-04-01

    The Daling Thrust (DT) delineates a zone of intense shear localization in the Lesser Himalayan Sequence (LHS) of the Darjeeling-Sikkim Himalaya. From microstructural studies of deformed quartzite samples, we show a transition in the dynamic recrystallization mechanism with increasing distance from the DT, dominated by grain boundary bulging (BLG) recrystallization closest to the DT, and progressively replaced by sub-grain rotation (SGR) recrystallization away from the thrust. The transition is marked by a characteristic variation in the fractal dimension (D) of grain boundaries, estimated from the area-perimeter method. For the BLG regime, D ≈ 1.046, which decreases significantly to a value as low as 1.025 for the SGR regime. Using the available thermal data for BLG and SGR recrystallization, we infer increasing deformation temperatures away from the DT in the hanging wall. Based on the quartz piezometer our estimates reveal strong variations in the flow stress (59.00 MPa to 16.00 MPa) over a distance of ~ 1.2 km from the DT. Deformation mechanism maps constructed for different temperatures indicate that the strain rates (10- 12 S- 1 to 10- 14 S- 1) comply with the geologically possible range. Finally, we present a mechanical model to provide a possible explanation for the cause of stress intensification along the DT.

  3. Mutations in Genes patA and patL of Anabaena sp. Strain PCC 7120 Result in Similar Phenotypes, and the Proteins Encoded by Those Genes May Interact ▿

    PubMed Central

    Liu, Jinjie; Wolk, C. Peter

    2011-01-01

    PatA resembles a response regulator protein with a defective DNA-binding domain, and PatL (All3305) is a pentapeptide repeat protein. A yeast two-hybrid library identified PatL as a protein with which PatA may interact. Heterocysts of patA and patL Anabaena sp. form nearly exclusively terminally in long filaments, further linking the genes. PMID:21890704

  4. Shut out of medicine in Canada, Dr. Leonora Howard King blazed a trail in China.

    PubMed Central

    Negodaeff-Tomsik, M

    1996-01-01

    After being denied the opportunity to study medicine and work at home, Dr. Leonora Howard King became Canada's first female medical missionary to China. Although she attempted to wear both the religious and secular hats handed her by the Women's Foreign Missionary Society, Howard King found that she was too busy meeting the medical needs of destitute women and children to proselytize. She won the favour of Chinese royalty, and after treating hundreds of wounded soldiers during the 1894-95 war with Japan became the first Western woman to become a mandarin, an honour bestowed by her adopted country. Images p1742-a PMID:8976342

  5. Construction and operation of the Howard T. Ricketts Laboratory.

    SciTech Connect

    Van Lonkhuyzen, R.; Stull, L.; Butler, J.; Chang, Y.; Allison, T.; O'Rourke, D.

    2006-01-01

    The National Institutes of Health (NIH) has proposed to partially fund the construction of the Howard T. Ricketts (HTR) regional biocontainment laboratory (RBL) by the University of Chicago at the U.S. Department of Energy's (DOE's) Argonne National Laboratory in Argonne, Illinois. The HTR Laboratory (HTRL) would be constructed, owned, and operated by the University of Chicago on land leased to it by DOE. The preferred project site is located north of Eastwood Drive and west of Outer Circle Road and is near the biological sciences building. This environmental assessment addresses the potential environmental effects resulting from construction and operation of the proposed facility. The proposed project involves the construction of a research facility with a footprint up to approximately 44,000 ft{sup 2} (4,088 m{sup 2}). The proposed building would house research laboratories, including Biosafety Level 2 and 3 biocontainment space, animal research facilities, administrative offices, and building support areas. The NIH has identified a need for new facilities to support research on potential bioterrorism agents and emerging and re-emerging infectious diseases, to protect the nation from such threats to public health. This research requires specialized laboratory facilities that are designed, managed, and operated to protect laboratory workers and the surrounding community from accidental exposure to agents. The proposed HTRL would provide needed biocontainment space to researchers and promote the advancement of knowledge in the disciplines of biodefense and emerging and re-emerging infectious diseases. Several alternatives were considered for the location of the proposed facility, as well as a no action alternative. The preferred alternative includes the construction of a research facility, up to 44,000 ft{sup 2} (4,088 m{sup 2}), at Argonne National Laboratory, a secure government location. Potential impacts to natural and cultural resources have been evaluated in

  6. 77 FR 72349 - SBI International, Inc. v. Mr. Howard Finkel c/o Cosco Container Lines; Notice of Filing of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-05

    ... From the Federal Register Online via the Government Publishing Office FEDERAL MARITIME COMMISSION SBI International, Inc. v. Mr. Howard Finkel c/o Cosco Container Lines; Notice of Filing of Complaint...,'' against Mr. Howard Finkel c/o Cosco Container Lines, hereinafter ``Respondent.'' Complainant alleges...

  7. Report of the Survey of Howard University: The Graduate School and Selected Ph.D. Programs.

    ERIC Educational Resources Information Center

    Office of Education (DHEW), Washington, DC.

    Results of the Office of Education's Bureau of Higher and Continuing Education's inspection of Howard University's Graduate School and the Ph.D. program are presented. The inspection was performed by conducting a survey of the administrative staff, the faculty, and student body, and by examining relevant facilities, laboratories, equipment,…

  8. 75 FR 44936 - Ochoco National Forest, Lookout Mountain Ranger District; Oregon; Howard Elliot Johnson Fuels and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service Ochoco National Forest, Lookout Mountain Ranger District; Oregon; Howard Elliot Johnson Fuels and Vegetation Management Project EIS Correction In notice document 2010-17803 beginning on...

  9. New Directions for Higher Education: Q&A with Muriel Howard on Public Higher Ed

    ERIC Educational Resources Information Center

    DiSalvio, Philip

    2014-01-01

    Nearly a year ago, "NEJHE" launched its "New Directions for Higher Education" series to examine emerging issues, trends, and ideas that have an impact on higher education policies, programs, and practices. In this installment, DiSalvio interviews Muriel A. Howard, president of the American Association of State Colleges and…

  10. Howard Zinn and the Struggle for Real History in the United States

    ERIC Educational Resources Information Center

    Whitson, James Anthony

    2009-01-01

    Criticisms of Howard Zinn's work on U.S. History are examined in the context of increasing emphasis on the idea of "American exceptionalism" in contemporary political and cultural discourse, and particularly the insertion of American exceptionalism into revised social studies standards for the state of Texas. Analysis focusing on the difference…

  11. Howard Community College 1986-87 Institutional Indicators for Proposed Operating Budget.

    ERIC Educational Resources Information Center

    Radcliffe, Susan; Novak, Virginia E.

    This report presents a series of graphs and tables, offering information on students, instruction, faculty, and finances at Howard Community College (HCC) in Columbia, Maryland. Part 1 provides profiles and projections of HCC's students, including a 10-year overview of full-time equivalent (FTE) enrollment, 1976-85; enrollment projections,…

  12. Howard Thurman: The Making of a Morehouse Man, 1919-1923

    ERIC Educational Resources Information Center

    Giles, Mark S.

    2006-01-01

    Since their founding in the late nineteenth and early twentieth centuries, historically Black colleges and universities (HBCUs) have represented the intellectual incubators, nurturing social-cultural environments, and professional launching pads of numerous black leaders, such as noted black theologian and revered Morehouse man, Howard Washington…

  13. The Future of Howard Community College. Report of the Commission on the Future.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD. Office of Planning and Evaluation.

    In October, 1998, the Howard Community College (Maryland) board of trustees created a Commission on the Future (COF) to help the College identify emerging issues, prepare for the future, and develop a vision of the College's role in the 21st century. Task forces were established to conduct research, interview experts, and debate how the college…

  14. Project Probes Digital Media's Effect on Ethics: Howard Gardner Leads Team Studying Youths' Web Norms

    ERIC Educational Resources Information Center

    Viadero, Debra

    2008-01-01

    Noted Harvard psychologist Howard Gardner is leading a team studying the social and ethical norms of young people on the Web. Known as the "GoodPlay Project," the study is being financed with a grant from the Chicago-based Catherine T. MacArthur Foundation. What researchers hope to do through the project is fill a gap in the burgeoning research…

  15. Revitalizing Politics Now and Then: Howard Zinn on Dissent, Democracy, and Education

    ERIC Educational Resources Information Center

    Cooley, Aaron

    2009-01-01

    This paper presents a discussion of Howard Zinn's intellectual and political ideas. Through the analysis of selections from his immense body of work, several interrelated themes emerge. Drawing more attention to these notions of dissent and democracy is crucial to revitalizing education at all levels and vital to advancing the public discourse…

  16. 75 FR 43138 - Ochoco National Forest, Lookout Mountain Ranger District; Oregon; Howard Elliot Johnson Fuels and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-23

    ... Forest Service Ochoco National Forest, Lookout Mountain Ranger District; Oregon; Howard Elliot Johnson... Maurice Evans, Acting District Ranger, Lookout Mountain District, Ochoco National Forest, 3160 NE. Third.... SUPPLEMENTARY INFORMATION: Purpose and Need. The Lookout Mountain Ranger District has determined that there is...

  17. Written Assignments for Abnormal Psychology at Howard Community College, Fall 1988.

    ERIC Educational Resources Information Center

    Bell, James

    Designed for students enrolled in an Abnormal Psychology course at Howard Community College (Maryland), this booklet explains the requirements for the course's writing assignments, which are designed to teach the skills of comparison and contrast, analysis, critical thinking, and synthesis. Following an overview of class assignments and…

  18. The Evolution of Creativity, Giftedness, and Multiple Intelligences: An Interview with Ellen Winner and Howard Gardner

    ERIC Educational Resources Information Center

    Henshon, Suzanna E.

    2006-01-01

    This article presents an interview with Ellen Winner and Howard Gardner. Winner is Professor of Psychology at Boston College, and Senior Research Associate at Project Zero, Harvard Graduate School of Education. Her research focuses on learning and cognition in the arts in typical and gifted children. She is the author of over 100 articles and…

  19. Black, White, Greek...Like Who?: Howard University Student Perceptions of a White Fraternity on Campus

    ERIC Educational Resources Information Center

    Hughey, Matthew W.

    2006-01-01

    On March 2, 1867, the Historically Black College or University (HBCU) Howard University (HU) was founded in Washington, D. C. Almost exactly one year later, the all white fraternity of Pi Kappa Alpha was founded at the University of Virginia. Over 100 years later, on February 18 2006, fifty-five HU students became charter members of Pi Kappa Alpha…

  20. Team Teaching Verbal, Mathematics, and Learning Skills. Howard University. The Center for Academic Reinforcement.

    ERIC Educational Resources Information Center

    Bartlett, Joan; Byrd, Roland

    Team teaching was used in three undergraduate courses to explore its potential for enhancing students' academic development. The courses were part of a program offered to freshmen with unrealized academic potential through the Howard University (District of Columbia) Center for Academic Reinforcement (CAR). A three-hour block of time was set aside…

  1. Commission on the Future of Howard Community College Progress Report, 2001.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD. Office of Planning and Evaluation.

    This Howard Community College (HCC) (Maryland) 2001 Progress Report makes assessments and recommendations in the following areas: (1) creating a world class learning organization; (2) collaboration with other educational organizations; (3) collaboration with business and industry; (4) economics and workforce development; (5) preparing students as…

  2. Commission on the Future of Howard Community College, Progress Report 2000.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD. Office of Planning and Evaluation.

    Presents the Commission on the Future of Howard Community College's (HCC) (Maryland) progress report 2000. Section 1, "Creating a World Class Learning Organization," presents strategic process and tactical action recommendations. Section 2, "Collaboration with Other Educational Organizations," discusses curricular and programmatic development,…

  3. Thermodynamics and Chemistry by Howard DeVoe

    NASA Astrophysics Data System (ADS)

    Gislason, Eric A.

    2001-09-01

    Prentice Hall: Upper Saddle River, NJ, 2001. 439 pp. ISBN 0-02-328741-1. $90.00. Thermodynamics is a deceptively difficult subject that few people master in their first exposure (typically in the junior-level physical chemistry course). Because of this, a clear, well-written textbook is always welcome to help students and teachers master this material. Such a book is Thermodynamics and Chemistry, by Howard DeVoe of the University of Maryland. This book is written as a one-semester textbook for senior undergraduates and graduate students who have had a previous course on the topic. This is a long book (400 pages of text, 25 pages of appendices), and it is unlikely that an instructor can cover everything in one semester. On the other hand this length does allow the author to cover topics such as a "liquid solution in a centrifugal field" that shorter books would omit. All important topics in chemical thermodynamics are covered. After two introductory chapters, the three laws of thermodynamics are taken up in Chapters 3 and 4. Pure substances, including phase transitions, are treated in Chapters 5 and 6. Chapter 7, "Mixtures," is a long chapter that covers, among other topics, partial molar quantities, activities, and activity coefficients. Chemical reactions are treated in Chapter 8 and there is a detailed discussion of equilibrium in various multicomponent systems in Chapter 9. The book is completed with a discussion of the phase rule (Chapter 10) and a brief chapter on galvanic cells. There are a number of things I like about this book. First, DeVoe takes great care in defining important thermodynamic words such as the thermodynamic state of a system. Similarly, he makes the distinction between process and path understandable, and this allows him to clearly define a reversible process as well as spontaneous and impossible processes. Section 4.1 then contains the sentence "An irreversible process is a spontaneous process whose reverse is an impossible process." This

  4. Thermodynamics and Chemistry by Howard DeVoe

    NASA Astrophysics Data System (ADS)

    Gislason, Eric A.

    2001-09-01

    Prentice Hall: Upper Saddle River, NJ, 2001. 439 pp. ISBN 0-02-328741-1. $90.00. Thermodynamics is a deceptively difficult subject that few people master in their first exposure (typically in the junior-level physical chemistry course). Because of this, a clear, well-written textbook is always welcome to help students and teachers master this material. Such a book is Thermodynamics and Chemistry, by Howard DeVoe of the University of Maryland. This book is written as a one-semester textbook for senior undergraduates and graduate students who have had a previous course on the topic. This is a long book (400 pages of text, 25 pages of appendices), and it is unlikely that an instructor can cover everything in one semester. On the other hand this length does allow the author to cover topics such as a "liquid solution in a centrifugal field" that shorter books would omit. All important topics in chemical thermodynamics are covered. After two introductory chapters, the three laws of thermodynamics are taken up in Chapters 3 and 4. Pure substances, including phase transitions, are treated in Chapters 5 and 6. Chapter 7, "Mixtures," is a long chapter that covers, among other topics, partial molar quantities, activities, and activity coefficients. Chemical reactions are treated in Chapter 8 and there is a detailed discussion of equilibrium in various multicomponent systems in Chapter 9. The book is completed with a discussion of the phase rule (Chapter 10) and a brief chapter on galvanic cells. There are a number of things I like about this book. First, DeVoe takes great care in defining important thermodynamic words such as the thermodynamic state of a system. Similarly, he makes the distinction between process and path understandable, and this allows him to clearly define a reversible process as well as spontaneous and impossible processes. Section 4.1 then contains the sentence "An irreversible process is a spontaneous process whose reverse is an impossible process." This

  5. Dividing the pie: differential dung pat size utilization by sympatric Haematobia irritans and Musca autumnalis.

    PubMed

    Fowler, F E; Mullens, B A

    2016-06-01

    Horn flies [Haematobia irritans (Diptera: Muscidae) (L.)] and face flies [Musca autumnalis (Diptera: Muscidae) De Geer] use the same larval resource, but their interactions are poorly studied. Dung pats (n = 350) were core sampled in the summers of 2012 and 2013 from irrigated pastures in Pomona, California, U.S.A. (34°03'N, 117°48'W) and held for face fly and horn fly emergence. Surface areas and estimated weights were recorded for each whole pat. Almost half (42.0%) of the pat cores yielded neither fly, 29.7% yielded horn flies only, 12.9% yielded face flies only and 15.4% yielded both flies. Of the fly-positive pats, surface area and mass were larger for face fly-occupied pats, whereas horn fly-occupied pats were smaller. Pats shared by the two species were intermediate. Horn flies per positive core were unaffected by the absence/presence of face flies, but half as many face flies emerged when pats were co-inhabited by horn flies. Face flies inhabited larger pats, which might better resist heating and drying, to which they are susceptible; horn flies inhabited a broad pat size range. Horn fly tolerance of lower dung moisture probably allows horn flies to colonize and survive in a wide range of pats in dry areas like southern California. PMID:26947576

  6. Use of modified Fraser's stain in Promoting Activity Test (PAT).

    PubMed

    Borràs, M

    1988-09-01

    The Promoting Activity Test (PAT) requires a staining procedure that allows rapid, accurate and reliable counting of mitotic figures. We propose use of Fraser's kernechtrot-crystal violet technique, but eliminating the picric-alcoholic differentiation to avoid fading. This modified protocol gives higher mitotic counts in adult mouse adrenal cortex than the hematoxylin-eosin originally used, especially with respect to less conspicuous prophases. PMID:2464217

  7. Myxomatosis: the introduction of the European rabbit flea Spilopsyllus cuniculi (Dale) into wild rabbit populations in Australia

    PubMed Central

    Sobey, W. R.; Conolly, Dorothy

    1971-01-01

    1. The European rabbit flea Spilopsyllus cuniculi (Dale) bred successfully in wild rabbits on three properties in New South Wales and, within two breeding seasons, almost every rabbit shot within a quarter of a mile of a release site was infested. 2. It was demonstrated that the flea transmitted myxoma virus in the field. 3. In areas where more than 75% of the rabbits shot at the beginning of the breeding season were flea-infested and myxoma virus was present, populations failed to show the expected summer build-up. PMID:5285938

  8. Flood-Inundation Maps for a 1.6-Mile Reach of Salt Creek, Wood Dale, Illinois

    USGS Publications Warehouse

    Soong, David T.; Murphy, Elizabeth A.; Sharpe, Jennifer B.

    2012-01-01

    Digital flood-inundation maps for a 1.6-mile reach of Salt Creek from upstream of the Chicago, Milwaukee, St. Paul & Pacific Railroad to Elizabeth Drive, Wood Dale, Illinois, were created by the U.S. Geological Survey (USGS) in cooperation with the DuPage County Stormwater Management Division. The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation/ depict estimates of the areal extent of flooding corresponding to selected water levels (gage heights) at the USGS streamgage on Salt Creek at Wood Dale, Illinois (station number 05531175). Current conditions at the USGS streamgage may be obtained on the Internet at http://waterdata.usgs.gov/usa/nwis/uv?05531175. In this study, flood profiles were computed for the stream reach by means of a one-dimensional unsteady flow Full EQuations (FEQ) model. The unsteady flow model was verified by comparing the rating curve output for a September 2008 flood event to discharge measurements collected at the Salt Creek at Wood Dale gage. The hydraulic model was then used to determine 14 water-surface profiles for gage heights at 0.5-ft intervals referenced to the streamgage datum and ranging from less than bankfull to approximately the highest recorded water level at the streamgage. The simulated water-surface profiles were then combined with a Geographic Information System (GIS) Digital Elevation Model (DEM) (derived from Light Detection and Ranging (LiDAR) data) in order to delineate the area flooded at each water level. The areal extent of the inundation was verified with high-water marks from a flood in July 2010 with a peak gage height of 14.08 ft recorded at the Salt Creek at Wood Dale gage. The availability of these maps along with Internet information regarding current gage height from USGS streamgages provide emergency management personnel and residents with information that is critical for flood response activities such as

  9. Overview of the High Performance Antiproton (HiPAT) Experiment

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Sims, William H.; Chakrabarti, Suman; Pearson, Boise; Fant, Wallace E.; Lewis, Raymond A.; Rodgers, Stephen (Technical Monitor)

    2002-01-01

    The annihilation of matter with antimatter represents the highest energy density of any known reaction, producing 10(exp 8) MJ/g, approximately 10 orders of magnitude more energy per unit mass than chemical based combustion. To take the first step towards using this energy for propulsion applications the NASA MSFC Propulsion Research Center (PRC) has initiated a research activity examining the storage of low energy antiprotons. Storage was identified as a key enabling technology since it builds the experience base necessary to understand the handling of antiprotons for virtually all utilization and high-density storage concepts. To address this need, a device referred to as the High Performance Antiproton Trap (HiPAT) is under development at the NASA MSFC PRC. The HiPAT is an electromagnetic system (Penning-Malmberg design) consisting of a 4 Tesla superconductor, a high voltage confinement electrode system (operation up to 20 KV), and an ultra high vacuum test section (operating in the 10(exp -12) torr range). The system was designed to be portable with an ultimate goal of maintaining 10(exp 12) charged particles with a half-life of 18 days. Currently, this system is being experimentally evaluated using normal matter ions which are cheap to produce and relatively easy to handle. These normal ions provide a good indication of overall trap behavior, with the exception of assessing annihilation losses. The ions are produced external to HiPAT using two hydrogen ion sources, with adjustable beam energy and current. Ion are transported in a beam line and controlled through the use of electrostatic optics. These optics serve to both focus and gate the incoming ions, providing microsecond-timed pulses that are dynamically captured by cycling the HiPAT electric containment field like a 'trap door'. The layout of this system more closely simulates the operations expected at an actual antiproton production facility where 'packets' of antiprotons with pulse widths measured in

  10. The cytoplasmic mRNA degradation factor Pat1 is required for rRNA processing.

    PubMed

    Muppavarapu, Mridula; Huch, Susanne; Nissan, Tracy

    2016-04-01

    Pat1 is a key cytoplasmic mRNA degradation factor, the loss of which severely increases mRNA half-lives. Several recent studies have shown that Pat1 can enter the nucleus and can shuttle between the nucleus and the cytoplasm. As a result, many nuclear roles have been proposed for Pat1. In this study, we analyzed four previously suggested nuclear roles of Pat1 and show that Pat1 is not required for efficient pre-mRNA splicing or pre-mRNA decay in yeast. However, lack of Pat1 results in accumulation of pre-rRNA processing intermediates. Intriguingly, we identified a novel genetic relationship between Pat1 and the rRNA decay machinery, specifically the exosome and the TRAMP complex. While the pre-rRNA processing intermediates that accumulate in the pat1 deletion mutant are, at least to some extent, recognized as aberrant by the rRNA degradation machinery, it is unlikely that these accumulations are the cause of their synthetic sick relationship. Here, we show that the dysregulation of the levels of mRNAs related to ribosome biogenesis could be the cause of the accumulation of the pre-rRNA processing intermediates. Although our results support a role for Pat1 in transcription, they nevertheless suggest that the primary cause of the dysregulated mRNA levels is most likely due to Pat1's role in mRNA decapping and mRNA degradation. PMID:26918764

  11. Perfluoroalkyl substances in the Daling River with concentrated fluorine industries in China: seasonal variation, mass flow, and risk assessment.

    PubMed

    Zhu, Zhaoyun; Wang, Tieyu; Meng, Jing; Wang, Pei; Li, Qifeng; Lu, Yonglong

    2015-07-01

    In the present study, we investigated concentrations and composition profiles of perfluoroalkyl substances (PFASs) in surface water from the Daling River in different seasons. The highest concentration of ΣPFASs (9540 ng L(-1)) and dominant homologues were found in surface water collected in summer. Perfluorobutanoic acid (PFBA), perfluorobutane sulfonate (PFBS), and perfluorooctanoic acid (PFOA) were the dominant PFASs in four seasons with a total contribution of over 90%. The discharge of two fluorine chemical industry parks was predicted to be the main contamination source of PFASs in the study area. The daily and annual mass flows were calculated according to data of detected PFAS concentrations and water discharge, and the annual mass loading of PFASs into the Bohai Sea from the Daling River reached to 461 kg year(-1). Hazard assessment indicated that concentrations of PFASs in study area had little risk to aquatic organisms and wildlife. However, the multiple health risk would be relatively high if the water was served as drinking water in the study area. PMID:25666478

  12. Unpacking Pat Parker: Intersections and Revolutions in "Movement in Black".

    PubMed

    Washburn, Amy

    2015-01-01

    This article explores Pat Parker's poem "Movement in Black." It examines the ways in which she emblematizes intersectionality and simultaneity as forms of revolution in struggles of self and society. It begins with a theoretical and historical apparatus to contextualize Parker as an artist and activist. Then it offers a literary analysis of the poem, focusing on themes of time and space, marginalization and movement, difference and power, visibility and invisibility, and history and memory. It argues that Parker uses autobiographical writing to fuse personal and political sites of resistance. PMID:26075685

  13. Short-Term Memory after All: Comment on Sederberg, Howard, and Kahana (2008)

    ERIC Educational Resources Information Center

    Usher, Marius; Davelaar, Eddy J.; Haarmann, Henk J.; Goshen-Gottstein, Yonatan

    2008-01-01

    P. B. Sederberg, M. W. Howard, and M. J. Kahana have proposed an updated version of the temporal-context model (TCM-A). In doing so, they accepted the challenge of developing a single-store model to account for the dissociations between short- and long-term recency effects that were reviewed by E. J. Davelaar, Y. Goshen-Gottstein, A. Ashkenazi, H.…

  14. Dale Reed with X-38 and a Subscale Model Used in Test Program

    NASA Technical Reports Server (NTRS)

    1997-01-01

    Dale Reed, a NASA engineer who worked on the original lifting-body research programs in the 1960s and 1970s, stands with a scale-model X-38 that was used in 1995 research flights, with a full-scale X-38 (80 percent of the size of a potential Crew Return Vehicle) behind him. The X-38 Crew Return Vehicle (CRV) research project is designed to develop the technology for a prototype emergency crew return vehicle, or lifeboat, for the International Space Station. The project is also intended to develop a crew return vehicle design that could be modified for other uses, such as a joint U.S. and international human spacecraft that could be launched on the French Ariane-5 Booster. The X-38 project is using available technology and off-the-shelf equipment to significantly decrease development costs. Original estimates to develop a capsule-type crew return vehicle were estimated at more than $2 billion. X-38 project officials have estimated that development costs for the X-38 concept will be approximately one quarter of the original estimate. Off-the-shelf technology is not necessarily 'old' technology. Many of the technologies being used in the X-38 project have never before been applied to a human-flight spacecraft. For example, the X-38 flight computer is commercial equipment currently used in aircraft and the flight software operating system is a commercial system already in use in many aerospace applications. The video equipment for the X-38 is existing equipment, some of which has already flown on the space shuttle for previous NASA experiments. The X-38's primary navigational equipment, the Inertial Navigation System/Global Positioning System, is a unit already in use on Navy fighters. The X-38 electromechanical actuators come from previous joint NASA, U.S. Air Force, and U.S. Navy research and development projects. Finally, an existing special coating developed by NASA will be used on the X-38 thermal tiles to make them more durable than those used on the space

  15. The Preschool Attention-Deficit/Hyperactivity Disorder Treatment Study (PATS) 6-Year Follow-Up

    ERIC Educational Resources Information Center

    Riddle, Mark A.; Yershova, Kseniya; Lazzaretto, Deborah; Paykina, Natalya; Yenokyan, Gayane; Greenhill, Laurence; Abikoff, Howard; Vitiello, Benedetto; Wigal, Tim; McCracken, James T.; Kollins, Scott H.; Murray, Desiree W.; Wigal, Sharon; Kastelic, Elizabeth; McGough, James J.; dosReis, Susan; Bauzo-Rosario, Audrey; Stehli, Annamarie; Posner, Kelly

    2013-01-01

    Objective: To describe the clinical course of attention-deficit/hyperactivity disorder (ADHD) symptom severity and diagnosis from ages 3 to 5 up to 9 to 12 years during a 6-year follow-up after the original Preschool ADHD Treatment Study (PATS). Method: A total of 207 participants (75% male) from the original PATS, assessed at baseline (mean age,…

  16. Rationale, Design, and Methods of the Preschool ADHD Treatment Study (PATS)

    ERIC Educational Resources Information Center

    Kollins, Scott; Greenhill, Laurence; Swanson, James; Wigal, Sharon; Abikoff, Howard; McCracken, James; Riddle, Mark; McGough, James; Vitiello, Benedetto; Wigal, Tim; Skrobala, Anne; Posner, Kelly; Ghuman, Jaswinder; Davies, Mark; Cunningham, Charles; Bauzo, Audrey

    2006-01-01

    Objective: To describe the rationale and design of the Preschool ADHD Treatment Study (PATS). Method: PATS was a National Institutes of Mental Health-funded, multicenter, randomized, efficacy trial designed to evaluate the short-term (5 weeks) efficacy and long-term (40 weeks) safety of methylphenidate (MPH) in preschoolers with…

  17. A Training Program for Pat Frisking Female Inmates for New York City Department of Correction Officers.

    ERIC Educational Resources Information Center

    Andrews, Brenda M.

    A training program was developed for male correction officers confronted with pat frisking female inmates. The training goal was to have correction officers in women's prisons identify female inmates' contraband and be able to determine alternatives for male correction officers who were confronted with pat frisking female inmates. The Dick and…

  18. HOWARD EISEN, JPL'S LEAD MECHANICAL TECHNICIAN, HOLDS MARS PATHFINDER 'SOJOURNER' ROVER 1:1 SCALE DU

    NASA Technical Reports Server (NTRS)

    1996-01-01

    The Mars Pathfinder 'Sojourner' rover l:l scale duplicate test vehicle is held by Howard Eisen, its lead mechanical technician from the Jet Propulsion Laboratory, with Kennedy Space Center's Vehicle Assembly Building looming in the background. The launch of NASA's Mars Pathfinder spacecraft aboard a McDonnell Douglas Delta II rocket is scheduled for Monday, Dec. 2, at 2:09:11 a.m. EST. This is a single instantaneous target launch time without a second opportunity on that day. Liftoff will occur from Pad B at Launch Complex 17 on Cape Canaveral Air Station, Fla. There is a 24-day launch opportunity which extends through Dec. 31.

  19. PAT Program Description. Parent-Child Programs. District 742 Community Education's Early Childhood Family Education Project [Revised].

    ERIC Educational Resources Information Center

    Hoodecheck, Jeanne; Kearns, Ellen

    Project PAT (Parents Are Teachers), a program for parents and their children from birth through 3 years, is described. PAT is a nationally validated Title III, Elementary and Secondary Education Act demonstration project in the St. Cloud Community Schools district of Minnesota. The focus of PAT is the quality of the parent-child relationship and…

  20. Issues in development, evaluation, and use of the NASA Preflight Adaptation Trainer (PAT)

    NASA Technical Reports Server (NTRS)

    Lane, Norman E.; Kennedy, Robert S.

    1988-01-01

    The Preflight Adaptation Trainer (PAT) is intended to reduce or alleviate space adaptation syndrome by providing opportunities for portions of that adaptation to occur under normal gravity conditions prior to space flight. Since the adaptation aspects of the PAT objectives involve modification not only of the behavior of the trainee, but also of sensiomotor skills which underly the behavioral generation, the defining of training objectives of the PAT utilizes four mechanisms: familiarization, demonstration, training and adaptation. These mechanisms serve as structural reference points for evaluation, drive the content and organization of the training procedures, and help to define the roles of the PAT instructors and operators. It was determined that three psychomotor properties are most critical for PAT evaluation: reliability; sensitivity; and relevance. It is cause for concern that the number of measures available to examine PAT effects exceed those that can be properly studied with the available sample sizes; special attention will be required in selection of the candidate measure set. The issues in PAT use and application within a training system context are addressed through linking the three training related mechanisms of familiarization, demonstration and training to the fourth mechanism, adaptation.

  1. Structural insights into HetR−PatS interaction involved in cyanobacterial pattern formation

    PubMed Central

    Hu, Hai-Xi; Jiang, Yong-Liang; Zhao, Meng-Xi; Cai, Kun; Liu, Sanling; Wen, Bin; Lv, Pei; Zhang, Yonghui; Peng, Junhui; Zhong, Hui; Yu, Hong-Mei; Ren, Yan-Min; Zhang, Zhiyong; Tian, Changlin; Wu, Qingfa; Oliveberg, Mikael; Zhang, Cheng-Cai; Chen, Yuxing; Zhou, Cong-Zhao

    2015-01-01

    The one-dimensional pattern of heterocyst in the model cyanobacterium Anabaena sp. PCC 7120 is coordinated by the transcription factor HetR and PatS peptide. Here we report the complex structures of HetR binding to DNA, and its hood domain (HetRHood) binding to a PatS-derived hexapeptide (PatS6) at 2.80 and 2.10 Å, respectively. The intertwined HetR dimer possesses a couple of novel HTH motifs, each of which consists of two canonical α-helices in the DNA-binding domain and an auxiliary α-helix from the flap domain of the neighboring subunit. Two PatS6 peptides bind to the lateral clefts of HetRHood, and trigger significant conformational changes of the flap domain, resulting in dissociation of the auxiliary α-helix and eventually release of HetR from the DNA major grove. These findings provide the structural insights into a prokaryotic example of Turing model. PMID:26576507

  2. Polyamine Metabolism Is Altered in Unpollinated Parthenocarpic pat-2 Tomato Ovaries1

    PubMed Central

    Fos, Mariano; Proaño, Karina; Alabadí, David; Nuez, Fernando; Carbonell, Juan; García-Martínez, José L.

    2003-01-01

    Facultative parthenocarpy induced by the recessive mutation pat-2 in tomato (Lycopersicon esculentum Mill.) depends on gibberellins (GAs) and is associated with changes in GA content in unpollinated ovaries. Polyamines (PAs) have also been proposed to play a role in early tomato fruit development. We therefore investigated whether PAs are able to induce parthenocarpy and whether the pat-2 mutation alters the content and metabolism of PAs in unpollinated ovaries. Application of putrescine, spermidine, and spermine to wild-type unpollinated tomato ovaries (cv Madrigal [MA/wt]) induced partial parthenocarpy. Parthenocarpic growth of MA/pat-2 (a parthenocarpic near-isogenic line to MA/wt) ovaries was negated by paclobutrazol (GA biosynthesis inhibitor), and this inhibition was counteracted by spermidine. Application of α-difluoromethyl-ornithine (-Orn) and/or α-difluoromethyl-arginine (-Arg), irreversible inhibitors of the putrescine biosynthesis enzymes Orn decarboxylase (ODC) and Arg decarboxylase, respectively, prevented growth of unpollinated MA/pat-2 ovaries. α-Difluoromethyl-Arg inhibition was counteracted by putrescine and GA3, whereas that of α-difluoromethyl-Orn was counteracted by GA3 but not by putrescine or spermidine. In unpollinated MA/pat-2 ovaries, the content of free spermine was significantly higher than in MA/wt ovaries. ODC activity was higher in pat-2 ovaries than in MA/wt. Transcript levels of genes encoding ODC and spermidine synthase were also higher in MA/pat-2. All together, these results strongly suggest that the parthenocarpic ability of pat-2 mutants depends on elevated PAs levels in unpollinated mutant ovaries, which correlate with an activation of the ODC pathway, probably as a consequence of elevated GA content in unpollinated pat-2 tomato ovaries. PMID:12529543

  3. Early development of PAT-SM6 for the treatment of melanoma.

    PubMed

    Hensel, Frank; Eckstein, Miriam; Rosenwald, Andreas; Brändlein, Stephanie

    2013-08-01

    Despite the recent development of novel therapies for patients with metastatic melanoma, this disease remains fatal in the majority of those who develop a relapse. Here, we report the preclinical and early clinical development of a novel IgM antibody PAT-SM6 that specifically binds to a cancer-specific isoform of glucose-regulated protein 78 (GRP78) and low-density lipoprotein. Finding a GRP78 cancer-specific form on the surface of cancer cells, but not normal cells in vivo, presents an opportunity for cancer-specific targeting. PAT-SM6 binding to the cell surface induces apoptosis in a variety of tumors, including melanoma. Recent studies show the specificity of PAT-SM6 binding to the surface of melanoma cells and primary tissue but not to normal tissue. They also confirm, for the first time, cell proliferation inhibition and apoptosis through classical apoptotic pathways as well as induction of lipid accumulation in melanoma cells. These in-vitro data are supported by positive in-vivo data using PAT-SM6 in a xenograft C8161 model. Furthermore, PAT-SM6 was well tolerated in pharmacokinetic/toxicology studies in monkeys. On the basis of these preclinical observations, a clinical study of PAT-SM6 was carried out in patients with 'in-transit' melanoma. Even with microdosing, histological analyses of tumor biopsies detected the presence of PAT-SM6 as well as apoptosis. Although there are many small molecules and monoclonal antibodies currently in clinical development for patients with melanoma, PAT-SM6 is the only therapeutic targeting the cancer-specific isoform of GRP78. These PAT-SM6 preclinical data and positive findings from the phase 1 safety study provide strong support for the further development of this novel antibody. PMID:23728394

  4. Pointing, acquisition and tracking (PAT) subsystems and components for optical space communication systems

    NASA Astrophysics Data System (ADS)

    Kern, R. H.; Kugel, U.

    1989-10-01

    The pointing, acquisition, and tracking (PAT) subsystem that will be incorporated by optical space communications transceivers must furnish very precise beam steering due to the highly collimated nature of the laser beams employed. The PAT subsystem must also be able to cover an angular rate of more than a full hemisphere, if it is intended for use on a LEO satellite which communicates with another in GEO. The present PAT is composed of a coarse-pointing assembly, a fine-pointing assembly, and a point-ahead assembly; their interactive operation is managed by a control law electronics unit. A CO2 laser is assumed to be the transmitter.

  5. Oracle Applications Patch Administration Tool (PAT) Beta Version

    Energy Science and Technology Software Center (ESTSC)

    2002-01-04

    PAT is a Patch Administration Tool that provides analysis, tracking, and management of Oracle Application patches. This includes capabilities as outlined below: Patch Analysis & Management Tool Outline of capabilities: Administration Patch Data Maintenance -- track Oracle Application patches applied to what database instance & machine Patch Analysis capture text files (readme.txt and driver files) form comparison detail report comparison detail PL/SQL package comparison detail SQL scripts detail JSP module comparison detail Parse and loadmore » the current applptch.txt (10.7) or load patch data from Oracle Application database patch tables (11i) Display Analysis -- Compare patch to be applied with current Oracle Application installed Appl_top code versions Patch Detail Module comparison detail Analyze and display one Oracle Application module patch. Patch Management -- automatic queue and execution of patches Administration Parameter maintenance -- setting for directory structure of Oracle Application appl_top Validation data maintenance -- machine names and instances to patch Operation Patch Data Maintenance Schedule a patch (queue for later execution) Run a patch (queue for immediate execution) Review the patch logs Patch Management Reports« less

  6. Oracle Applications Patch Administration Tool (PAT) Beta Version

    SciTech Connect

    2002-01-04

    PAT is a Patch Administration Tool that provides analysis, tracking, and management of Oracle Application patches. This includes capabilities as outlined below: Patch Analysis & Management Tool Outline of capabilities: Administration Patch Data Maintenance -- track Oracle Application patches applied to what database instance & machine Patch Analysis capture text files (readme.txt and driver files) form comparison detail report comparison detail PL/SQL package comparison detail SQL scripts detail JSP module comparison detail Parse and load the current applptch.txt (10.7) or load patch data from Oracle Application database patch tables (11i) Display Analysis -- Compare patch to be applied with current Oracle Application installed Appl_top code versions Patch Detail Module comparison detail Analyze and display one Oracle Application module patch. Patch Management -- automatic queue and execution of patches Administration Parameter maintenance -- setting for directory structure of Oracle Application appl_top Validation data maintenance -- machine names and instances to patch Operation Patch Data Maintenance Schedule a patch (queue for later execution) Run a patch (queue for immediate execution) Review the patch logs Patch Management Reports

  7. The UK-DALE dataset, domestic appliance-level electricity demand and whole-house demand from five UK homes.

    PubMed

    Kelly, Jack; Knottenbelt, William

    2015-01-01

    Many countries are rolling out smart electricity meters. These measure a home's total power demand. However, research into consumer behaviour suggests that consumers are best able to improve their energy efficiency when provided with itemised, appliance-by-appliance consumption information. Energy disaggregation is a computational technique for estimating appliance-by-appliance energy consumption from a whole-house meter signal. To conduct research on disaggregation algorithms, researchers require data describing not just the aggregate demand per building but also the 'ground truth' demand of individual appliances. In this context, we present UK-DALE: an open-access dataset from the UK recording Domestic Appliance-Level Electricity at a sample rate of 16 kHz for the whole-house and at 1/6 Hz for individual appliances. This is the first open access UK dataset at this temporal resolution. We recorded from five houses, one of which was recorded for 655 days, the longest duration we are aware of for any energy dataset at this sample rate. We also describe the low-cost, open-source, wireless system we built for collecting our dataset. PMID:25984347

  8. Transport of short-chain perfluoroalkyl acids from concentrated fluoropolymer facilities to the Daling River estuary, China.

    PubMed

    Wang, Pei; Lu, Yonglong; Wang, Tieyu; Zhu, Zhaoyun; Li, Qifeng; Zhang, Yueqing; Fu, Yaning; Xiao, Yang; Giesy, John P

    2015-07-01

    After global commercialization of short-chain perfluoroalkyl acids (PFAAs) as substitutes to conventional long-chain PFAAs by the major manufacturers, two fluorine industry parks for production of short-chain PFAAs located in the Daling River Basin of northern China have developed rapidly in the last few years. This study provides a systematic assessment of sources, emissions, transportation, and potential risks of the PFAAs in this area. The C4 perfluorobutane sulfonic acid (PFBS) and perfluorobutanoic acid (PFBA) were the predominant short-chain PFAAs in river water, with maximum concentrations of 2.90 and 1.35 μg/L, respectively. Park 1 equipped with a telomerization process was identified to be the source of linear and branched mixtures of PFBS, PFBA, and perfluorooctanoic acid (PFOA), while park 2 with an electrochemical fluorination process (ECF) was identified to be the source of linear and branched mixtures of PFBS and PFOA. Partition coefficients between water and sediment were consistent for C4-C8 perfluoroalkyl carboxylic acids (PFCAs) but directly proportional to C9-C11 PFCAs and perfluoroalkyl sulfonic acids (PFSAs). Analysis on the health risk of PFBS and PFBA suggested that they were not without risk since short chain PFAAs are known to be recalcitrant during water treatment. PMID:25616381

  9. The UK-DALE dataset, domestic appliance-level electricity demand and whole-house demand from five UK homes

    PubMed Central

    Kelly, Jack; Knottenbelt, William

    2015-01-01

    Many countries are rolling out smart electricity meters. These measure a home’s total power demand. However, research into consumer behaviour suggests that consumers are best able to improve their energy efficiency when provided with itemised, appliance-by-appliance consumption information. Energy disaggregation is a computational technique for estimating appliance-by-appliance energy consumption from a whole-house meter signal. To conduct research on disaggregation algorithms, researchers require data describing not just the aggregate demand per building but also the ‘ground truth’ demand of individual appliances. In this context, we present UK-DALE: an open-access dataset from the UK recording Domestic Appliance-Level Electricity at a sample rate of 16 kHz for the whole-house and at 1/6 Hz for individual appliances. This is the first open access UK dataset at this temporal resolution. We recorded from five houses, one of which was recorded for 655 days, the longest duration we are aware of for any energy dataset at this sample rate. We also describe the low-cost, open-source, wireless system we built for collecting our dataset. PMID:25984347

  10. Solid state laser communications in space (SOLACOS) position, acquisition, and tracking (PAT) subsystem implementation

    NASA Astrophysics Data System (ADS)

    Flemmig, Joerg; Pribil, Klaus

    1994-09-01

    This paper presents the concept and implementation aspects of the Pointing, Acquisition and Tracking Subsystem (PAT) which is developed in the frame of the SOLACOS (Solid State Laser Communications in Space) program.

  11. Air transport of plutonium metal: content expansion initiative for the plutonium air transportable (PAT01) packaging

    SciTech Connect

    Caviness, Michael L; Mann, Paul T

    2010-01-01

    The National Nuclear Security Administration (NNSA) has submitted an application to the Nuclear Regulatory Commission (NRC) for the air shipment of plutonium metal within the Plutonium Air Transportable (PAT-1) packaging. The PAT-1 packaging is currently authorized for the air transport of plutonium oxide in solid form only. The INMM presentation will provide a limited overview of the scope of the plutonium metal initiative and provide a status of the NNSA application to the NRC.

  12. Air transport of plutonium metal : content expansion initiative for the Plutonium Air Transportable (PAT-1) packaging.

    SciTech Connect

    Mann, Paul T.; Caviness, Michael L.; Yoshimura, Richard Hiroyuki

    2010-06-01

    The National Nuclear Security Administration (NNSA) has submitted an application to the Nuclear Regulatory Commission (NRC) for the air shipment of plutonium metal within the Plutonium Air Transportable (PAT-1) packaging. The PAT-1 packaging is currently authorized for the air transport of plutonium oxide in solid form only. The INMM presentation will provide a limited overview of the scope of the plutonium metal initiative and provide a status of the NNSA application to the NRC.

  13. GeoPAT: A toolbox for pattern-based information retrieval from large geospatial databases

    NASA Astrophysics Data System (ADS)

    Jasiewicz, Jarosław; Netzel, Paweł; Stepinski, Tomasz

    2015-07-01

    Geospatial Pattern Analysis Toolbox (GeoPAT) is a collection of GRASS GIS modules for carrying out pattern-based geospatial analysis of images and other spatial datasets. The need for pattern-based analysis arises when images/rasters contain rich spatial information either because of their very high resolution or their very large spatial extent. Elementary units of pattern-based analysis are scenes - patches of surface consisting of a complex arrangement of individual pixels (patterns). GeoPAT modules implement popular GIS algorithms, such as query, overlay, and segmentation, to operate on the grid of scenes. To achieve these capabilities GeoPAT includes a library of scene signatures - compact numerical descriptors of patterns, and a library of distance functions - providing numerical means of assessing dissimilarity between scenes. Ancillary GeoPAT modules use these functions to construct a grid of scenes or to assign signatures to individual scenes having regular or irregular geometries. Thus GeoPAT combines knowledge retrieval from patterns with mapping tasks within a single integrated GIS environment. GeoPAT is designed to identify and analyze complex, highly generalized classes in spatial datasets. Examples include distinguishing between different styles of urban settlements using VHR images, delineating different landscape types in land cover maps, and mapping physiographic units from DEM. The concept of pattern-based spatial analysis is explained and the roles of all modules and functions are described. A case study example pertaining to delineation of landscape types in a subregion of NLCD is given. Performance evaluation is included to highlight GeoPAT's applicability to very large datasets. The GeoPAT toolbox is available for download from

  14. Capacity-Building for African American Mental Health Training and Research: Lessons from the Howard-Dartmouth Collaborative Summer School

    ERIC Educational Resources Information Center

    Hipolito, Maria M. S.; Malik, Mansoor; Carpenter-Song, Elizabeth; Whitley, Rob

    2012-01-01

    Background: Many psychiatric residents have traditionally received little-or-no training in cross cultural approaches to psychiatric training and research. Method: The Dartmouth-Howard Collaboration summer school training program had a 5-year grant to explore approaches to enhancing understanding of cultural factors in mental health treatment and…

  15. What to Teach about Asia: Howard Wilson and the Committee on Asiatic Studies in the 1940s

    ERIC Educational Resources Information Center

    Shaffer, Robert

    2001-01-01

    In 1942, Howard Wilson, a professor at the Harvard Graduate School of Education and the editor of the Harvard Educational Review, called for the "easternization of America," in reaction to what he called the "glib" talk for years about the "westernization of Asia." Funded by the Rockefeller Foundation, Wilson's Committee, Committee on Asiatic…

  16. College of New Rochelle Response to: "Adult Learning, Higher Education, and the Economics of Unused Capacity," by Howard R. Bowen.

    ERIC Educational Resources Information Center

    Kelly, Dorothy Ann

    Perspectives on adult students at the College of New Rochelle are offered in response to Howard R. Bowen's address entitled, "Adult Learning, Higher Education, and the Economics of Unused Capacity." The College of New Rochelle suggests a modest modification of Bowen's thesis that there should be efforts toward full integration of programs for…

  17. Communicating with New and Existing Markets. A Task Force of Howard Community College's 1998-1999 Commission on the Future.

    ERIC Educational Resources Information Center

    Howard Community Coll., Columbia, MD.

    This is a report from a task force formed by Howard Community College (Maryland) to examine existing and future markets. The task force also explored ways to use marketing strategies to attract potential customers to the college. The task force recommends that the college use its strengths to attract customers, such as its commitment to open…

  18. On the Economics of American Higher Education: A Bibliography of Howard R. Bowen. Public Administration Series: Bibliography P-1105.

    ERIC Educational Resources Information Center

    Quay, Richard H., Comp.

    A bibliography of approximately 150 materials authored, coauthored, or edited by Howard R. Bowen from 1935 through 1982 on the economics of American higher education is presented. Specific topics include the following: the wide disparity among colleges in educational cost per student; costs colleges spend per student; adult learning, higher…

  19. Mosquitoes, flies and dental cavities: Dr. Howard Riley Raper's public campaign to prevent toothache.

    PubMed

    Christen, Arden G; Christen, Joan A

    2010-01-01

    Dr. Howard Riley Raper (1886-1978) was an early oral health pioneer and dental roentgenology faculty member of the Indiana Dental College (IDC) who single-handedly introduced key concepts in radiology to dentistry. Due to his efforts, IDC became in 1910-11 the first dental school to have a regular course in dental radiology. Virtually all American dental schools soon added this subject to their regular curriculum. Raper's text, Elementary and Dental Radiography (1913) became the first comprehensive student textbook of dental X-ray diagnosis. In his 1933 Blue Book entitled, The New Aim in the Care of the Teeth, Raper elaborated upon his mission to prevent caries, by comparing the insidious damages of tooth decay with the threat of insect-borne disease. PMID:20968230

  20. Water Vapor Measurements by Howard University Raman Lidar during the WAVES 2006 Campaign

    NASA Technical Reports Server (NTRS)

    Adam, M.; Demoz, B. B.; Whiteman, D. N.; Venable, D. D.; Joseph E.; Gambacorta, A.; Wei, J.; Shephard, M. W.; Miloshevich, L. M.; Barnet, C. D.; Herman, R. L.; Fitzgibbon, J.; Connell, R.

    2009-01-01

    Retrieval of water vapor mixing ratio using the Howard University Raman Lidar is presented with emphasis on three aspects: i) performance of the lidar against collocated radiosondes and Raman lidar, ii) investigation of the atmospheric state variables when poor agreement between lidar and radiosondes values occurred and iii) a comparison with satellite-based measurements. The measurements were acquired during the Water Vapor Validation Experiment Sondes/Satellites 2006 field campaign. Ensemble averaging of water vapor mixing ratio data from ten night-time comparisons with Vaisala RS92 radiosondes shows on average an agreement within 10 % up to approx. 8 km. A similar analysis of lidar-to-lidar data of over 700 profiles revealed an agreement to within 20 % over the first 7 km (10 % below 4 km). A grid analysis, defined in the temperature - relative humidity space, was developed to characterize the lidar - radiosonde agreement and quantitatively localizes regions of strong and weak correlations as a function of altitude, temperature or relative humidity. Three main regions of weak correlation emerge: i) regions of low relative humidity and low temperature, ii) moderate relative humidity at low temperatures and iii) low relative humidity at moderate temperatures. Comparison of Atmospheric InfraRed Sounder and Tropospheric Emission Sounder satellites retrievals of moisture with that of Howard University Raman Lidar showed a general agreement in the trend but the formers miss a lot of the details in atmospheric structure due to their low resolution. A relative difference of about 20 % is usually found between lidar and satellites measurements.

  1. The Howard University Hospital Experience with Routineized HIV Screening: A Progress Report*

    PubMed Central

    Scott, Victor F.; Sitapati, Amy; Martin, Sayyida; Summers, Pamela; Washington, Michael; Daniels, Fernando; Mouton, Charles; Bonney, George; Apprey, Victor; Webster, Virginia; Smith, Avemaria; Mountvarner, Geoffrey; Daftary, Monica; Maxwell, Celia J.

    2009-01-01

    Background: Howard University Hospital (HUH) is the first hospital in the nation to have instituted a hospital-wide routine rapid HIV screening campaign as recommended by the CDC for healthcare settings. Methods: HUH developed a protocol and implemented a hospital-wide routine HIV screening in October 2006. Rapid oral fluid-based HIV testing was conducted throughout the hospital using the OraSure OraQuick Advance Rapid HIV-1/2 Antibody Test. Patients with a preliminarily reactive test result were either referred for confirmatory testing or offered a Western Blot confirmatory test on-site and referred for follow-up care. This is a report on the progress of this program for the first eight months. Results: Of the 9,817 patients offered HIV testing, 5,642 consented. The mean age of the screened population was 40.7 years. Ninety percent of the patients screened were black and 55% were female. A preliminarily reactive test result was identified in 139 patients for a seroprevalence rate of 2.46%. Of these patients, 136, or 98% were black; 63% were male and 37% were female. HIV prevalence in the overall sample, among blacks, and among both black males and females peaked in the 40–54 year old age group. Challenges were experienced initially in securing confirmatory tests. Conclusions: Hospital-wide routine HIV screening is both possible and productive. The routine HIV screening campaign instituted at Howard University Hospital has identified a significant number of previously unidentified HIV positive persons. Success in assuring confirmatory testing and transition to care improved as time progressed. PMID:19768195

  2. Palaeomagnetism of the Howards Pass Zn-Pb deposits, Yukon, Canada

    NASA Astrophysics Data System (ADS)

    Kawasaki, K.; Symons, D. T. A.

    2012-07-01

    The Howards Pass district is located in the Selwyn Basin, Yukon. The district consists of >15 laminated clastic-dominated (CD) sphalerite-galena (Zn-Pb) deposits in the 'zinc corridor' that trends northwest-southeast and extends for ˜35 km. The stratiform mineralized zone, the Active Member, is hosted in carbonaceous cherts and black shales of the Early Silurian Road River Group. Using mostly thermal and then alternating field step demagnetization isolated a stable characteristic remanent magnetization (ChRM) by the core-magnetization-angle method from 18 sites (339 specimens) in 18 variably oriented exploration drill hole cores from 6 Zn-Pb mineralized panels. Rock magnetic analyses show that the main remanence carriers are single- or pseudosingle-domain pyrrhotite and titanomagnetite. The deposits' mean ChRM direction yields a pole position of either ˜170 Ma on the North American apparent polar wander path or ˜162 Ma on a corrected path for the Intermontane Belt (IMB) terranes. A negative palaeomagnetic fold test indicates that the mineralization's ChRM is post-folding, setting a minimum age for regional metamorphic deformation of ˜170 ± 20 Ma and supporting an Early Jurassic arrival for the IMB's collision and accretion to North America. An autochthonous or para-autochthonous North American tectonic model is favoured for the ChRM of the metamorphosed Selwyn Basin strata rather than an allochthonous IMB model. Further, the palaeomagnetic age indicates that the coarse-grained Zn-Pb mineralization in fine fractures that cut the laminated fine-grained Zn-Pb mineralization at Howards Pass at least was formed by remobilization during Middle Jurassic metamorphism.

  3. Enfermedad diarreica aguda por Escherichia coli patógenas en Colombia

    PubMed Central

    Gómez-Duarte, Oscar G.

    2014-01-01

    Resumen Las cepas de E. coli patógenas intestinales son causas importantes de la enfermedad diarreica aguda (EDA) en niños menores de 5 años en América Latina, África y Asia y están asociadas a alta mortalidad en niños en las comunidades más pobres de África y el Sudeste Asiático. Estudios sobre el papel de las E. coli patógenas intestinales en la EDA infantil en Colombia y otros países de América Latina son limitados debido a la carencia de ensayos para detección de estos patógenos en los laboratorios clínicos de centros de salud. Estudios recientes han reportado la detección de E. coli patógenas intestinales en Colombia, siendo la E. coli enterotoxigénica la cepa más frecuentemente asociada a diarrea en niños menores de 5 años. Otros patógenos detectados en estos pacientes incluyen las E. coli enteroagregativa, enteropatógena, productora de toxina Shiga, y de adherencia difusa. Con base en estudios que reportan la presencia de E. coli productora de toxina Shiga y E. coli enteroagregativa en carnes y vegetales en supermercados, se cree que productos alimentarios contaminados contribuyen a la transmisión de estos patógenos y a la infección del huésped susceptible. Más estudios son necesarios para evaluar los mecanismos de transmisión, el impacto en la epidemiologia de la EDA, y las pautas de manejo y prevención de estos patógenos que afectan la población pediátrica en Colombia. PMID:25491457

  4. Clinical Usefulness of Watch-PAT for Assessing the Surgical Results of Obstructive Sleep Apnea Syndrome

    PubMed Central

    Park, Chong Yoon; Hong, Joon Hyeong; Lee, Jae Heon; Lee, Kyu Eun; Cho, Hyun Sang; Lim, Su Jin; Kwak, Jin Wook; Kim, Kyung Soo; Kim, Hyun Jik

    2014-01-01

    Objective: This study aimed to assess the accuracy and clinical efficacy of a wrist-worn device that is based on peripheral arterial tonometry (watch-PAT) to evaluate the surgical results of obstructive sleep apnea (OSA) syndrome subjects. Study Design and Method: Thirty-five subjects who were diagnosed with OSA and underwent sleep surgeries such as septoplasty, tonsillectomy, or uvuloplasty to correct their airway collapse, participated in this study; the watch-PAT-derived respiratory disturbance index (RDI), apnea and hypopnea index (AHI), lowest oxygen saturation, and valid sleep time were measured after the sleep surgery. Results: The present study showed that RDI (32.8 ± 10.7 vs 14.8 ± 7.5), AHI (30.3 ± 8.6 vs 13.4 ± 8.2 events/h), lowest oxygen saturation (78.2% ± 8.4% vs 90.5% ± 7.1%), and valid sleep time (329.1 ± 47.2 min and a postoperative value of 389.1 ± 50.1 min) recovered to within a normal range after surgery in 28 subjects. In addition, good agreement was found between watch-PAT-derived factors and visual analogue scales for changes in subjective symptoms, such as snoring, apnea, and daytime somnolence. Seven of the 35 subjects showed no improvement for their subjective symptoms and complained of snoring and apnea after surgery. We found that the RDI and AHI of those 7 subjects were not reduced, and the changes between pre- and postoperative values which were measured with watch-PAT were minimal. Their postoperative lowest oxygen saturation and valid sleep time were not elevated per the watch-PAT. The results support a strong correlation between the findings from watch-PAT and improved symptoms after surgical correction of an airway collapse. Conclusions: Our study provides evidence that the factors measured by the watch-PAT might be reliable indicators of symptomatic changes in OSA subjects after sleep surgery and also shows that the watch-PAT is a highly sensitive portable device for estimating treatment results in OSA. Citation: Park CY

  5. PAT: a protein analysis toolkit for integrated biocomputing on the web

    PubMed Central

    Gracy, Jérôme; Chiche, Laurent

    2005-01-01

    PAT, for Protein Analysis Toolkit, is an integrated biocomputing server. The main goal of its design was to facilitate the combination of different processing tools for complex protein analyses and to simplify the automation of repetitive tasks. The PAT server provides a standardized web interface to a wide range of protein analysis tools. It is designed as a streamlined analysis environment that implements many features which strongly simplify studies dealing with protein sequences and structures and improve productivity. PAT is able to read and write data in many bioinformatics formats and to create any desired pipeline by seamlessly sending the output of a tool to the input of another tool. PAT can retrieve protein entries from identifier-based queries by using pre-computed database indexes. Users can easily formulate complex queries combining different analysis tools with few mouse clicks, or via a dedicated macro language, and a web session manager provides direct access to any temporary file generated during the user session. PAT is freely accessible on the Internet at . PMID:15980554

  6. Simulation model and on-ground performances validation of the PAT system for SILEX program

    NASA Astrophysics Data System (ADS)

    Cosson, Francois; Doubrere, Patrick; Perez, Eric

    1991-06-01

    A description of the simulation model and the on-ground performances validation of the Acquisition and Tracking (PAT) subsystem and of the European SILEX program is presented. The SILEX scenario is composed of two terminals mounted on LEO orbit (SPOT 4) and geostationary orbit (ARTEMIS) spacecrafts. The PAT subsystem is in charge of the initialization of the link, of the tracking of the incoming beam and of the pointing of the emitted laser beam. A short description of the retained strategies (phases of the PAT mission and operating modes) and functional architecture (control laws and sampling frequencies) is presented. In order to validate the PAT performance assessment, a complete simulation software is developed. The model (equipment modeling, dynamical simulation of the terminal structure, and operating modes management) and the associated outputs are examined in detail. The chosen design and architecture of the PAT validation on a system testbed (STB) are described. The validation of a pointing system for optical intersatellite communication, thanks to the combination of simulations and STB measurements, is shown.

  7. PAT proteins, an ancient family of lipid droplet proteins that regulate cellular lipid stores.

    PubMed

    Bickel, Perry E; Tansey, John T; Welte, Michael A

    2009-06-01

    The PAT family of lipid droplet proteins includes 5 members in mammals: perilipin, adipose differentiation-related protein (ADRP), tail-interacting protein of 47 kDa (TIP47), S3-12, and OXPAT. Members of this family are also present in evolutionarily distant organisms, including insects, slime molds and fungi. All PAT proteins share sequence similarity and the ability to bind intracellular lipid droplets, either constitutively or in response to metabolic stimuli, such as increased lipid flux into or out of lipid droplets. Positioned at the lipid droplet surface, PAT proteins manage access of other proteins (lipases) to the lipid esters within the lipid droplet core and can interact with cellular machinery important for lipid droplet biogenesis. Genetic variations in the gene for the best-characterized of the mammalian PAT proteins, perilipin, have been associated with metabolic phenotypes, including type 2 diabetes mellitus and obesity. In this review, we discuss how the PAT proteins regulate cellular lipid metabolism both in mammals and in model organisms. PMID:19375517

  8. Heterocyst-Specific Expression of patB, a Gene Required for Nitrogen Fixation in Anabaena sp. Strain PCC 7120

    PubMed Central

    Jones, Kathryn M.; Buikema, William J.; Haselkorn, Robert

    2003-01-01

    The patB gene product is required for growth and survival of the filamentous cyanobacterium Anabaena sp. strain PCC 7120 in the absence of combined nitrogen. A patB::gfp fusion demonstrated that this gene is expressed exclusively in heterocysts. patB mutants have a normal initial pattern of heterocyst spacing along the filament but differentiate excess heterocysts after several days in the absence of combined nitrogen. Expression of hetR and patS, two critical regulators of the heterocyst development cascade, are normal for patB mutants, indicating that patB acts downstream of them in the differentiation pathway. A patB deletion mutant suffers an almost complete cessation of growth and nitrogen fixation within 24 h of combined nitrogen removal. In contrast, a new PatB mutant that is defective in its N-terminal ferredoxin domain, or a previously described mutant that has a frameshift removing its C-terminal helix-turn-helix domain, grows very slowly and differentiates multiple contiguous heterocysts under nitrogen-deficient conditions. PMID:12644502

  9. Project-based Modules from two STEM Learning Teams in Howard County, Maryland

    NASA Astrophysics Data System (ADS)

    Griffiths, L. N.; Bradley, L. A.

    2011-12-01

    In 2009, two Maryland school districts-Howard County Public School System and Prince George's County Public Schools-and the Goddard Space Flight Center of the National Aeronautics and Space Administration (NASA) partnered with the National Commission on Teaching and America's Future (NCTAF) to develop NASA 21st Century Learning Studios. In 2010, NCTAF expanded the program to include Learning Studios at two additional Maryland school districts (Anne Arundel County Public Schools and Baltimore County Public Schools), partnering with the United States Naval Academy and the University of Maryland. Overall, the focus of these Learning Studios is to combine the expertise of scientists with that of educators through Learning Teams to improve teaching and learning in science, technology, engineering and mathematics (STEM) fields, while delivering project-based modules to be implemented in other school districts. The focus of this paper is to summarize the experience and outcomes from two Learning Teams from the Howard County Public School System. STEM Learning Teams were established at Centennial High School and Hammond High School in Maryland. Each Team worked together for two years to create interdisciplinary units of study for their students with a focus on Earth Science. To maximize student interest, teachers worked with NASA scientists five times a year to develop four learning modules using practical examples and incorporating real scientific observations. A weathering and erosion module challenges students to collect appropriate field observations and determine erosion and deposition rates in a nearby lake. A plate tectonics module requires students to use measures of plate motion from the National Oceanic and Atmospheric Administration to estimate rates of convergence in southern Asia. A third module for lessons in climate change requires students to find open source climate data, determine changes in the atmosphere and estimate anthropogenic impacts. A follow

  10. Healing mysteries: An interview with Howard Hall, PhD, PsyD. Interview by Sheldon Lewis.

    PubMed

    Hall, Howard

    2007-01-01

    Dr Howard Hall is an associate professor in the department of pediatrics at Case Western Reserve University (CWRU) and an attending doctor in the division of behavioral pediatrics at Rainbow Babies and Children's Hospital in Cleveland, Ohio. Dr Hall holds a PhD in Experimental Psychology from Princeton University and a PsyD in Clinical Psychology from Rutgers University. He has conducted research and taught courses in clinical and multicultural psychology and maintains a clinical practice using hypnosis and other mind-body approaches to healing at CWRU. Howard Hall has been recognized as a leader in the field of clinical psychoneuroimmunology and conducted pioneering research on the effects of hypnosis on immune responses. In recent years, he has studied energy-based rapid wound healing as demonstrated by Sufi practitioners. Recently, Sheldon Lewis, Editor-in-Chief of Advances in Mind-Body Medicine, spoke with Dr Hall about his work. PMID:20671340

  11. RF Manipulation and Detection of Protons in the High Performance Antiproton Trap (HiPAT) Experiment

    NASA Technical Reports Server (NTRS)

    Martin, James; Lewis, Raymond A.; Pearson, J. Boise; Sims, W. Herb; Chakrabarti, Suman; Fant, Wallace E.; Stan McDonald

    2003-01-01

    HiPAT is a Penning-Malmberg trap designed to contain 10(exp 12) particles diagnostics of the plasma. Destructive particle detection is performed by extracting trapped ions from HiPAT and colliding them with a microchannel plate detector (providing number and energy information). The improved RF system has been used to detect various plasma modes for both electron and ion plasmas in the two traps at MSFC, including axd, cyclotron, and diocotron modes. New diagnostics are also being added to HiPAT to measure the axial density distribution of the trapped cloud to match measured RF plasma modes to plasma conditions. In addition, plasma modeling efforts have been started using the XOOPIC code.

  12. Overview of the High Performance Antiproton Trap (HiPAT) Experiment

    NASA Technical Reports Server (NTRS)

    Martin, James; Chakrabarti, Suman; Pearson, Boise; Sims, W. Herbert; Lewis, Raymond; Fant, Wallace; Rodgers, Stephen (Technical Monitor)

    2002-01-01

    A general overview of the High Performance Antiproton Trap (HiPAT) Experiment is presented. The topics include: 1) Why Antimatter? 2) HiPAT Applicability; 3) Approach-Goals; 4) HiPAT General Layout; 5) Sizing For Containment; 6) Laboratory Operations; 7) Vacuum System Cleaning; 8) Ion Production Via Electron Gun; 9) Particle Capture Via Ion Sources; 10) Ion Beam Steering/Focusing; 11) Ideal Ion Stacking Sequence; 12) Setup For Dynamic Capture; 13) Dynamic Capture of H(+) Ions; 14) Dynamic Capture; 15) Radio Frequency Particle Detection; 16) Radio Frequency Antenna Modeling; and 17) R.F. Stabilization-Low Frequencies. A short presentation of propulsion applications of Antimatter is also given. This paper is in viewgraph form.

  13. B. C. Canyon field, Howard County, Texas: An ancient analogy to modern tropical tower karst terrains

    SciTech Connect

    Mozynski, D.C.; Reid, A.M. )

    1992-04-01

    Late in the early deposition of sediments in Canyon field, a series of glacio-eustatically controlled sea level lowstands resulted in a carbonate buildup seaward of the Horseshoe Atoll in Howard County, Texas. The resulting satellite reef tract consists of fringing boundstone; high-energy shelf grainstones; lower energy shelf packstones and wackestones; and thin, highstand, black shales and mudstones. The original extent and thickness of deposits were extensively modified during karstification coincident with successive sea level lowstands. The resulting paleotopographic landforms appear to be similar to tower karst features of Puerto Rico. During the beginning of each sea level highstand, the paleoterrain was modified by erosion. The basinal foreshelf conglomerates resulting from initial highstand erosion contain dipping strata that commonly can be detected by the dipmeter tool. The mechanism for the formation of these strata may be depositional or the result of diagenetic alteration of the rock fabric in the burial environment. Using dipmeter data, an uneconomic producer has been offset by one of the better producing wells in the field.

  14. Principles and Practices Fostering Inclusive Excellence: Lessons from the Howard Hughes Medical Institute's Capstone Institutions.

    PubMed

    DiBartolo, Patricia Marten; Gregg-Jolly, Leslie; Gross, Deborah; Manduca, Cathryn A; Iverson, Ellen; Cooke, David B; Davis, Gregory K; Davidson, Cameron; Hertz, Paul E; Hibbard, Lisa; Ireland, Shubha K; Mader, Catherine; Pai, Aditi; Raps, Shirley; Siwicki, Kathleen; Swartz, Jim E

    2016-01-01

    Best-practices pedagogy in science, technology, engineering, and mathematics (STEM) aims for inclusive excellence that fosters student persistence. This paper describes principles of inclusivity across 11 primarily undergraduate institutions designated as Capstone Awardees in Howard Hughes Medical Institute's (HHMI) 2012 competition. The Capstones represent a range of institutional missions, student profiles, and geographical locations. Each successfully directed activities toward persistence of STEM students, especially those from traditionally underrepresented groups, through a set of common elements: mentoring programs to build community; research experiences to strengthen scientific skill/identity; attention to quantitative skills; and outreach/bridge programs to broaden the student pool. This paper grounds these program elements in learning theory, emphasizing their essential principles with examples of how they were implemented within institutional contexts. We also describe common assessment approaches that in many cases informed programming and created traction for stakeholder buy-in. The lessons learned from our shared experiences in pursuit of inclusive excellence, including the resources housed on our companion website, can inform others' efforts to increase access to and persistence in STEM in higher education. PMID:27562960

  15. The effects of PAT on the Savannah River ecosystem, particularly fisheries

    SciTech Connect

    Patrick, R.

    1994-03-01

    The main purpose of this study was to determine whether or not the pre-startup activities at K-Reactor, i.e., Power Ascension Testing (PAT), have caused damage because of temperature rises in the Savannah River. Therefore, the biological studies were mainly aimed at providing information as to changes that might cause the damage of the fish population, and to other important organisms in the ecosystem. To determine if deleterious effects had occurred, one had to review the past studies to determine the condition and diversity of aquatic life before these PAT studies started. Therefore old reports were reviewed and a current study made in 1992.

  16. The PATS Peer Support Program: Prevention/Early Intervention for Adolescents Who Have a Parent with Mental Illness

    ERIC Educational Resources Information Center

    Hargreaves, John; Bond, Lyndal; O'Brien, Matt; Forer, Danielle; Davies, Liz

    2008-01-01

    PATS (Paying Attention to Self) is a peer support program for adolescent children of parents with a diagnosed mental illness. The program aims to promote positive mental health, reduce the likelihood of mental health difficulties, increase young people's coping skills and empower them to meet their own and their families' needs. PATS combines peer…

  17. Identification of homologues to the pathogenicity factor Pat-1, a putative serine protease of Clavibacter michiganensis subsp. michiganensis.

    PubMed

    Burger, Annette; Gräfen, Ines; Engemann, Jutta; Niermann, Erik; Pieper, Martina; Kirchner, Oliver; Gartemann, Karl-Heinz; Eichenlaub, Rudolf

    2005-01-01

    Hybridization of Clavibacter michiganensis subsp. michiganensis total DNA against the pathogenicity gene pat-1 indicated the presence of pat-1 homologous nucleotide sequences on the chromosome and on plasmid pCM2. Isolation of the corresponding DNA fragments and nucleotide sequence determination showed that there are three pat-1 homologous genes: chpA (chromosome) and phpA and phpB (plasmid pCM2). The gene products share common characteristics, i.e. a signal sequence for Sec-dependent secretion, a serine protease motif, and six cysteine residues at conserved positions. Gene chpA located on the chromosome is a pseudogene since it contains a translational stop codon after 97 of 280 amino acids. In contrast to pat-1, cloning of the plasmid encoded homologs phpA and phpB into the avirulent plasmid free Cmm strain CMM100 did not result in a virulent phenotype. So far, no proteolytic activity could be demonstrated for Pat-1, however, site specific mutagenesis of pat-1 showed that the serine residue in the motif GDSGG is required for the virulent phenotype of pat-1 and thus Pat-1 could be a functional protease. PMID:16255147

  18. Design and Fabrication of Cryostat Interface and Electronics for High Performance Antimatter Trap (HI-PAT)

    NASA Technical Reports Server (NTRS)

    Smith, Gerald A.

    1999-01-01

    Included in Appendix I to this report is a complete set of design and assembly schematics for the high vacuum inner trap assembly, cryostat interfaces and electronic components for the MSFC HI-PAT. Also included in the final report are summaries of vacuum tests, and electronic tests performed upon completion of the assembly.

  19. THz spectroscopy: An emerging technology for pharmaceutical development and pharmaceutical Process Analytical Technology (PAT) applications

    NASA Astrophysics Data System (ADS)

    Wu, Huiquan; Khan, Mansoor

    2012-08-01

    As an emerging technology, THz spectroscopy has gained increasing attention in the pharmaceutical area during the last decade. This attention is due to the fact that (1) it provides a promising alternative approach for in-depth understanding of both intermolecular interaction among pharmaceutical molecules and pharmaceutical product quality attributes; (2) it provides a promising alternative approach for enhanced process understanding of certain pharmaceutical manufacturing processes; and (3) the FDA pharmaceutical quality initiatives, most noticeably, the Process Analytical Technology (PAT) initiative. In this work, the current status and progress made so far on using THz spectroscopy for pharmaceutical development and pharmaceutical PAT applications are reviewed. In the spirit of demonstrating the utility of first principles modeling approach for addressing model validation challenge and reducing unnecessary model validation "burden" for facilitating THz pharmaceutical PAT applications, two scientific case studies based on published THz spectroscopy measurement results are created and discussed. Furthermore, other technical challenges and opportunities associated with adapting THz spectroscopy as a pharmaceutical PAT tool are highlighted.

  20. Teacher's PAT? Multiple-Role Principal-Agent Theory, Education Politics, and Bureaucrat Power

    ERIC Educational Resources Information Center

    Vanhuysse, Pieter; Sulitzeanu-Kenan, Raanan

    2009-01-01

    This article aims to contribute to current debates about political power and agency relationships in education and other public sectors. In a recent clarion call for a major redirection of political principal-agent theories (PAT), Terry Moe has argued that standard information asymmetries ought no longer to be regarded as the sole foundation of…

  1. Comparing EndoPAT and BIOPAC measurement of vascular responses to mental stress.

    PubMed

    Martin, Elizabeth A; Nelson, Rebecca E; Felmlee-Devine, M Donna; Brown, Troy E; Lerman, Amir

    2011-06-01

    There are currently no comparison measurements of stress-induced changes in vascular function during acute mental stress tests to measurements made by BIOPAC MP150 systems technology, a standard polygraph device used to detect deception during polygraph examinations in military or law enforcement applications. Vascular responses to reactive hyperaemia and acute mental stress in 25 healthy subjects were measured by both peripheral arterial tonometry (EndoPAT) and a blood pressure cuff attached to a pressure transducer (BIOPAC) and compared. Reactive hyperaemia was performed at baseline and following three acute mental stress tests. There was no difference in vascular reactivity at baseline and following acute mental stress, as measured by EndoPAT or BIOPAC systems (p > 0·05). Mental stress ratios measured by EndoPAT were significantly different than those measured by BIOPAC (p < 0·01). These data suggest that EndoPAT measurements of vascular responses to acute mental stress may be more specific and sensitive than measurements using the BIOPAC system. PMID:21671245

  2. Pat Conroy's "Gutter Language": "Prince of Tides" in a Lowcountry High School.

    ERIC Educational Resources Information Center

    White, Robert A.

    1992-01-01

    Describes the controversy sparked by Pat Conroy's novel "The Prince of Tides" when it was included in a reading list for an advanced-placement eleventh grade English class. Discusses Conroy's approach to writing and his experience as an unconventional teacher. (PRA)

  3. Draft Genome Sequence of the Beer Spoilage Bacterium Megasphaera cerevisiae Strain PAT 1T

    PubMed Central

    Kutumbaka, Kirthi K.; Pasmowitz, Joshua; Mategko, James; Reyes, Dindo; Friedrich, Alex; Han, Sukkyun; Martens-Habbena, Willm; Neal-McKinney, Jason; Janagama, Harish K.; Nadala, Cesar

    2015-01-01

    The genus Megasphaera harbors important spoilage organisms that cause beer spoilage by producing off flavors, undesirable aroma, and turbidity. Megasphaera cerevisiae is mainly found in nonpasteurized low-alcohol beer. In this study, we report the draft genome of the type strain of the genus, M. cerevisiae strain PAT 1T. PMID:26358606

  4. Draft Genome Sequence of the Beer Spoilage Bacterium Megasphaera cerevisiae Strain PAT 1T.

    PubMed

    Kutumbaka, Kirthi K; Pasmowitz, Joshua; Mategko, James; Reyes, Dindo; Friedrich, Alex; Han, Sukkyun; Martens-Habbena, Willm; Neal-McKinney, Jason; Janagama, Harish K; Nadala, Cesar; Samadpour, Mansour

    2015-01-01

    The genus Megasphaera harbors important spoilage organisms that cause beer spoilage by producing off flavors, undesirable aroma, and turbidity. Megasphaera cerevisiae is mainly found in nonpasteurized low-alcohol beer. In this study, we report the draft genome of the type strain of the genus, M. cerevisiae strain PAT 1(T). PMID:26358606

  5. Reconstruction of undersampled radial PatLoc imaging using Total Generalized Variation

    PubMed Central

    Knoll, Florian; Schultz, Gerrit; Bredies, Kristian; Gallichan, Daniel; Zaitsev, Maxim; Hennig, Jürgen; Stollberger, Rudolf

    2016-01-01

    In the case of radial imaging with nonlinear spatial encoding fields, a prominent star-shaped artifact has been observed if a spin distribution is encoded with an undersampled trajectory. This work presents a new iterative reconstruction method based on the total generalized variation (TGV), which reduces this artifact. For this approach, a sampling operator (as well as its adjoint) is needed that maps data from PatLoc k-space to the final image space. It is shown that this can be realized as a Type-3 non-uniform FFT, which is implemented by a combination of a Type-1 and Type-2 non-uniform FFT. Using this operator, it is also possible to implement an iterative conjugate gradient (CG) SENSE based method for PatLoc reconstruction, which leads to a significant reduction of computation time in comparison to conventional PatLoc image reconstruction methods. Results from numerical simulations and in-vivo PatLoc measurements with as few as 16 radial projections are presented, which demonstrate significant improvements in image quality with the TGV based approach. PMID:22847824

  6. FunPat: function-based pattern analysis on RNA-seq time series data

    PubMed Central

    2015-01-01

    Background Dynamic expression data, nowadays obtained using high-throughput RNA sequencing, are essential to monitor transient gene expression changes and to study the dynamics of their transcriptional activity in the cell or response to stimuli. Several methods for data selection, clustering and functional analysis are available; however, these steps are usually performed independently, without exploiting and integrating the information derived from each step of the analysis. Methods Here we present FunPat, an R package for time series RNA sequencing data that integrates gene selection, clustering and functional annotation into a single framework. FunPat exploits functional annotations by performing for each functional term, e.g. a Gene Ontology term, an integrated selection-clustering analysis to select differentially expressed genes that share, besides annotation, a common dynamic expression profile. Results FunPat performance was assessed on both simulated and real data. With respect to a stand-alone selection step, the integration of the clustering step is able to improve the recall without altering the false discovery rate. FunPat also shows high precision and recall in detecting the correct temporal expression patterns; in particular, the recall is significantly higher than hierarchical, k-means and a model-based clustering approach specifically designed for RNA sequencing data. Moreover, when biological replicates are missing, FunPat is able to provide reproducible lists of significant genes. The application to real time series expression data shows the ability of FunPat to select differentially expressed genes with high reproducibility, indirectly confirming high precision and recall in gene selection. Moreover, the expression patterns obtained as output allow an easy interpretation of the results. Conclusions A novel analysis pipeline was developed to search the main temporal patterns in classes of genes similarly annotated, improving the sensitivity of

  7. Application of a novel phosphinothricin N-acetyltransferase (RePAT) gene in developing glufosinate-resistant rice.

    PubMed

    Cui, Ying; Liu, Ziduo; Li, Yue; Zhou, Fei; Chen, Hao; Lin, Yongjun

    2016-01-01

    Currently, only few glufosinate-resistant genes are available for commercial application. Thus, developing novel glufosinate-resistant genes with commercial feasibility is extremely important and urgent for agricultural production. In this study, we transferred a newly isolated RePAT gene into a japonica rice variety Zhonghua11, resulting in a large number of independent T0 transgenic plants, most of which grew normally under high-concentration glufosinate treatment. Four transgenic plants with one intact RePAT expression cassette integrated into the intergenic region were selected. Agronomic performances of their T2 progenies were investigated, and the results suggested that the expression of RePAT had no adverse effect on the agronomic performance. Definite glufosinate resistance of the selected transgenic plants was further confirmed to be related to the expression of RePAT by assay on the medium and qRT-PCR. The inheritance and expression of RePAT in two transgenic plants were confirmed to be stable. Finally, the two-year field assay of glufosinate resistance suggested that the agronomic performance of the transgenic plant (PAT11) was not affected by high dosage of glufosinate (5000 g/ha). Collectively, our study proves the high resistance of a novel gene RePAT to glufosinate and provides a glufosiante-resistant rice variety with agricultural application potential. PMID:26879398

  8. Application of a novel phosphinothricin N-acetyltransferase (RePAT) gene in developing glufosinate-resistant rice

    PubMed Central

    Cui, Ying; Liu, Ziduo; Li, Yue; Zhou, Fei; Chen, Hao; Lin, Yongjun

    2016-01-01

    Currently, only few glufosinate-resistant genes are available for commercial application. Thus, developing novel glufosinate-resistant genes with commercial feasibility is extremely important and urgent for agricultural production. In this study, we transferred a newly isolated RePAT gene into a japonica rice variety Zhonghua11, resulting in a large number of independent T0 transgenic plants, most of which grew normally under high-concentration glufosinate treatment. Four transgenic plants with one intact RePAT expression cassette integrated into the intergenic region were selected. Agronomic performances of their T2 progenies were investigated, and the results suggested that the expression of RePAT had no adverse effect on the agronomic performance. Definite glufosinate resistance of the selected transgenic plants was further confirmed to be related to the expression of RePAT by assay on the medium and qRT-PCR. The inheritance and expression of RePAT in two transgenic plants were confirmed to be stable. Finally, the two-year field assay of glufosinate resistance suggested that the agronomic performance of the transgenic plant (PAT11) was not affected by high dosage of glufosinate (5000 g/ha). Collectively, our study proves the high resistance of a novel gene RePAT to glufosinate and provides a glufosiante-resistant rice variety with agricultural application potential. PMID:26879398

  9. Social contract of academic medical centres to the community: Dr Howard Atwood Kelly (1858-1943), a historical perspective.

    PubMed

    Allen, Paul

    2016-05-01

    Academic medical centres have traditionally been bastions of teaching and research. Outreach to the community at large and involvement in community affairs have sometimes been lacking in the overall mission and activities of academic medical centres. This paper provides an historical perspective first on the numerous achievements of a physician and surgeon and then on the topic of involvement in community affairs by reviewing the many contributions of America's pioneer gynaecological surgeon and one of the four physician founders of the Johns Hopkins Hospital and School of Medicine in 1889 - Dr Howard Atwood Kelly. PMID:24906403

  10. Pat1 protects centromere-specific histone H3 variant Cse4 from Psh1-mediated ubiquitination

    PubMed Central

    Mishra, Prashant K.; Guo, Jiasheng; Dittman, Lauren E.; Haase, Julian; Yeh, Elaine; Bloom, Kerry; Basrai, Munira A.

    2015-01-01

    Evolutionarily conserved histone H3 variant Cse4 and its homologues are essential components of specialized centromere (CEN)-specific nucleosomes and serve as an epigenetic mark for CEN identity and propagation. Cse4 is a critical determinant for the structure and function of the kinetochore and is required to ensure faithful chromosome segregation. The kinetochore protein Pat1 regulates the levels and spatial distribution of Cse4 at centromeres. Deletion of PAT1 results in altered structure of CEN chromatin and chromosome segregation errors. In this study, we show that Pat1 protects CEN-associated Cse4 from ubiquitination in order to maintain proper structure and function of the kinetochore in budding yeast. PAT1-deletion strains exhibit increased ubiquitination of Cse4 and faster turnover of Cse4 at kinetochores. Psh1, a Cse4-specific E3-ubiquitin ligase, interacts with Pat1 in vivo and contributes to the increased ubiquitination of Cse4 in pat1∆ strains. Consistent with a role of Psh1 in ubiquitination of Cse4, transient induction of PSH1 in a wild-type strain resulted in phenotypes similar to a pat1∆ strain, including a reduction in CEN-associated Cse4, increased Cse4 ubiquitination, defects in spatial distribution of Cse4 at kinetochores, and altered structure of CEN chromatin. Pat1 interacts with Scm3 and is required for its maintenance at kinetochores. In conclusion, our studies provide novel insights into mechanisms by which Pat1 affects the structure of CEN chromatin and protects Cse4 from Psh1-mediated ubiquitination for faithful chromosome segregation. PMID:25833709

  11. Comparative sound velocity measurements between porous rock and fully-dense material under crustal condition: The cases of Darley Dale sandstone and copper block

    NASA Astrophysics Data System (ADS)

    Kung, J.; Chien, Y. V.; Wu, W.; Dong, J.; Chang, Y.; Tsai, C.; Yang, M.; Wang, K.

    2012-12-01

    Previous studies showed that the voids and their geometry in the sedimentary rocks have great influence on the compressibility of rock, which reflects on its elastic velocities. Some models were developed to discuss the relations among velocity, porosity and void geometry. Therefore, the information of porosity, and void geometry and its distribution in rock is essential for understanding how the elastic properties of porous rocks affected by their poregeometry. In this study, we revisited a well-studied porous rock, Darley Dale sandstone, which has been studied by different groups with different purposes. Most of them are the deformation experiments. Different from previous studies, we measured the sound velocity of Darley dale sandstone under hydrostatic conditions. Also, we employed different techniques to investigate the pore geometry and porosity of Darley Dale sandstone to gain the insight of velocity changing behavior under the crustal conditions. Here, we measured a fully-dense copper block for a comparison. We performed X-ray CT scanning (XCT) to image the pore space of sandstone to construct the 3-D image of pore geometry, distribution and the pore size. The CT image data are allowed us to estimate the porosity of sandstone, too. One the other hand, the porosity of sample was measured using imbibitions method at ambient conditions and helium porosimeter at high pressure (up to 150 MPa). A set of specimens were cored from Darley Dale sandstone block. P and S wave velocities of specimens were measured at ambient conditions. We also performed high pressure velocity measurements on a selected rock specimen and a copper block up to 150 MPa under dry condition. Porosity of a set of rock specimens measured by imbibitions method was spanned from 6% to 15%, largely distributed within a range of 8%-11%. Compared the porosity obtained from three different techniques, imbibitions method, helium porosimeter and XCT, values from those measurements are in good agreement

  12. Walter Max Dale (formerly Deutsch) (1894-1969): pioneer and eminent radiobiochemist at the Christie Hospital and Holt Radium Institute, Manchester.

    PubMed

    Shreeve, David R

    2010-05-01

    The political upheaval in Germany in 1933 and subsequent movement of medical scholars with the support of the Rockefeller Foundation allowed Manchester to benefit from the arrival of Dr Walter Deutsch, later known as Dr Walter Dale. His research background enabled him to develop a radiobiochemistry laboratory at the Christie Hospital and Holt Radium Institute where he became a world authority on the effects of X-rays on enzymes and also the protective effect of additional solutes. In 1959 he initiated and then edited the International Journal of Radiation Biology. By the time of his retirement in 1962 the strength of his research resulted in his laboratory being recognized by the Medical Research Council. PMID:20519710

  13. Physiological and Behavioral Responses of Horses to Wither Scratching and Patting the Neck When Under Saddle.

    PubMed

    Thorbergson, Zoë W; Nielsen, Sharon G; Beaulieu, Rodney J; Doyle, Rebecca E

    2016-01-01

    Riding is considered to be an arousing activity for horses. It has been suggested that wither scratching may be a more useful tool for relaxation compared with the common practice of neck patting. In the current study, 18 horses were exposed to 3 treatments, including control or no interaction, neck patting, and wither scratching, for 1 min each following a short obstacle course. Heart rate, heart rate variability, and a variety of behaviors were measured in the horses. Wither scratching produced a significantly longer duration of relaxed-type behaviors. Wither scratching could be a useful tool to help a horse relax while under saddle. Additionally, the study identified 2 ear positions that may be useful for future research in horse behavior. PMID:26958705

  14. PAT-1 safety analysis report addendum author responses to request for additional information.

    SciTech Connect

    Weiner, Ruth F.; Schmale, David T.; Kalan, Robert J.; Akin, Lili A.; Miller, David Russell; Knorovsky, Gerald Albert; Yoshimura, Richard Hiroyuki; Lopez, Carlos; Harding, David Cameron; Jones, Perry L.; Morrow, Charles W.

    2010-09-01

    The Plutonium Air Transportable Package, Model PAT-1, is certified under Title 10, Code of Federal Regulations Part 71 by the U.S. Nuclear Regulatory Commission (NRC) per Certificate of Compliance (CoC) USA/0361B(U)F-96 (currently Revision 9). The National Nuclear Security Administration (NNSA) submitted SAND Report SAND2009-5822 to NRC that documented the incorporation of plutonium (Pu) metal as a new payload for the PAT-1 package. NRC responded with a Request for Additional Information (RAI), identifying information needed in connection with its review of the application. The purpose of this SAND report is to provide the authors responses to each RAI. SAND Report SAND2010-6106 containing the proposed changes to the Addendum is provided separately.

  15. PAT-2 (Plutonium Air-Transportable Model 2) safety analysis report

    SciTech Connect

    Andersen, J.A.; Davis, E.J.; Duffey, T.A.; Dupree, S.A.; George, O.L. Jr.; Ortiz, Z.

    1981-07-01

    The PAT-2 package is designed for the safe transport of plutonium and/or uranium in small quantities. The PAT-2 package is resistant to severe accidents, including that of a high-speed jet aircraft crash, and is designed to withstand such environments as extreme impact, crushing, puncturing and slashing loads, severe hydrocarbon-fueled fires, and deep underwater immersion, with no escape of contents. The package meets the requirements of 10 CFR 71 for Fissile Class I packages with a cargo of 15 grams of Pu-239, or other isotopic forms described herein, not to exceed 2 watts of thermal activity. This SAR presents design and oprational information including evaluations and analyses, test results, operating procedures, maintenance, and quality assurance information.

  16. Review of the High Performance Antiproton Trap (HiPAT) Experiment

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Pearson, J. Boise; Sims, W. Herb; Chakrabarti, Suman; Fant, Wallace E.; McDonald, Stan

    2003-01-01

    Many space propulsion concepts exist that use matter-antimatter reactions. Current antiproton production rates are enough to conduct proof-of-principle evaluation of these concepts. One enabling technology for such experiments is portable storage of low energy antiprotons, to transport antiprotons to experimental facilities. To address this need, HiPAT is being developed, with a design goal of containing 10(exp 12) particles for up to 18 days. HiPAT is a Penning-Malmberg trap with a 4 Tesla superconductor, 20kV electrodes, radio frequency (RF) network, and 10(exp -13) Torr vacuum. 'Normal' matter is being used to evaluate the system. An electron beam ionizes background gas in situ, and particle beams are captured dynamically. The experiment examines ion storage lifetimes, RF plasma diagnostics, charge exchange with background gases, and dynamic ion beam capture.

  17. Quantifying Beetle-Mediated Effects on Gas Fluxes from Dung Pats

    PubMed Central

    Penttilä, Atte; Slade, Eleanor M.; Simojoki, Asko; Riutta, Terhi; Minkkinen, Kari; Roslin, Tomas

    2013-01-01

    Agriculture is one of the largest contributors of the anthropogenic greenhouse gases (GHGs) responsible for global warming. Measurements of gas fluxes from dung pats suggest that dung is a source of GHGs, but whether these emissions are modified by arthropods has not been studied. A closed chamber system was used to measure the fluxes of carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) from dung pats with and without dung beetles on a grass sward. The presence of dung beetles significantly affected the fluxes of GHGs from dung pats. Most importantly, fresh dung pats emitted higher amounts of CO2 and lower amounts of CH4 per day in the presence than absence of beetles. Emissions of N2O showed a distinct peak three weeks after the start of the experiment – a pattern detected only in the presence of beetles. When summed over the main grazing season (June–July), total emissions of CH4 proved significantly lower, and total emissions of N2O significantly higher in the presence than absence of beetles. While clearly conditional on the experimental conditions, the patterns observed here reveal a potential impact of dung beetles on gas fluxes realized at a small spatial scale, and thereby suggest that arthropods may have an overall effect on gas fluxes from agriculture. Dissecting the exact mechanisms behind these effects, mapping out the range of conditions under which they occur, and quantifying effect sizes under variable environmental conditions emerge as key priorities for further research. PMID:23940758

  18. X-PAT: a multiplatform patient referral data management system for small healthcare institution requirements.

    PubMed

    Masseroli, Marco; Marchente, Mario

    2008-07-01

    We present X-PAT, a platform-independent software prototype that is able to manage patient referral multimedia data in an intranet network scenario according to the specific control procedures of a healthcare institution. It is a self-developed storage framework based on a file system, implemented in eXtensible Markup Language (XML) and PHP Hypertext Preprocessor Language, and addressed to the requirements of limited-dimension healthcare entities (small hospitals, private medical centers, outpatient clinics, and laboratories). In X-PAT, healthcare data descriptions, stored in a novel Referral Base Management System (RBMS) according to Health Level 7 Clinical Document Architecture Release 2 (CDA R2) standard, can be easily applied to the specific data and organizational procedures of a particular healthcare working environment thanks also to the use of standard clinical terminology. Managed data, centralized on a server, are structured in the RBMS schema using a flexible patient record and CDA healthcare referral document structures based on XML technology. A novel search engine allows defining and performing queries on stored data, whose rapid execution is ensured by expandable RBMS indexing structures. Healthcare personnel can interface the X-PAT system, according to applied state-of-the-art privacy and security measures, through friendly and intuitive Web pages that facilitate user acceptance. PMID:18632322

  19. Drosophila PAT1 is required for Kinesin-1 to transport cargo and to maximize its motility

    PubMed Central

    Loiseau, Philippe; Davies, Tim; Williams, Lucy S.; Mishima, Masanori; Palacios, Isabel M.

    2010-01-01

    Kinesin heavy chain (KHC), the force-generating component of Kinesin-1, is required for the localization of oskar mRNA and the anchoring of the nucleus in the Drosophila oocyte. These events are crucial for the establishment of the anterior-posterior and dorsal-ventral axes. KHC is also essential for the localization of Dynein and for all ooplasmic flows. Interestingly, oocytes without Kinesin light chain show no major defects in these KHC-dependent processes, suggesting that KHC binds its cargoes and is activated by a novel mechanism. Here, we shed new light on the molecular mechanism of Kinesin function in the germline. Using a combination of genetic, biochemical and motor-tracking studies, we show that PAT1, an APP-binding protein, interacts with Kinesin-1, functions in the transport of oskar mRNA and Dynein and is required for the efficient motility of KHC along microtubules. This work suggests that the role of PAT1 in cargo transport in the cell is linked to PAT1 function as a positive regulator of Kinesin motility. PMID:20630947

  20. Ion Dynamic Capture Experiments With The High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James; Lewis, Raymond; Chakrabarti, Suman; Sims, William H.; Pearson, J. Boise; Fant, Wallace E.

    2002-01-01

    To take the first step towards using the energy produced from the matter-antimatter annihilation for propulsion applications, the NASA Marshall Space Flight Center (MSFC) Propulsion Research Center (PRC) has initiated a research activity examining the storage of low energy antiprotons. The High Performance Antiproton Trap (HiPAT) is an electromagnetic system (Penning-Malmberg design) consisting of a 4 Tesla superconductor, a high voltage electrode confinement system, and an ultra high vacuum test section. It has been designed with an ultimate goal of maintaining 10(exp 12) charged particles with a half-life of 18 days. Currently, this system is being evaluated experimentally using normal matter ions that are cheap to produce, relatively easy to handle, and provide a good indication of overall trap behavior (with the exception of assessing annihilation losses). The ions are produced via a positive hydrogen ion source and transported to HiPAT in a beam line equipped with electrostatic optics. The optics serve to both focus and gate the incoming ions, providing microsecond-timed beam pulses that are dynamically captured by cycling the HiPAT forward containment field like a "trap door". Initial dynamic capture experiments have been successfully performed with beam energy and currents set to 1.9 kV and 23 micro-amps, respectively. At these settings up to 2x10(exp 9) ions have been trapped during a single dynamic cycle.

  1. Ion Storage Tests with the High Performance Antimatter Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Chakrabarti, Suman; Pearson, Boise; Schafer, Charles (Technical Monitor)

    2002-01-01

    The NASA/Marshall Space Flight Centers (NASA/MSFC) Propulsion Research Center (PRC) is evaluating an antiproton storage system, referred to as the High Performance Antiproton Trap (HiPAT). This interest stems from the sheer energy represented by matter/antimatter annihilation process with has an energy density approximately 10 order of magnitude above that of chemical propellants. In other terms, one gram of antiprotons contains the equivalent energy of approximately 23 space shuttle external tanks or ET's (each ET contains roughly 740,000 kgs of fuel and oxidizer). This incredible source of stored energy, if harnessed, would be an enabling technology for deep space mission where both spacecraft weight and propulsion performance are key to satisfying aggressive mission requirements. The HiPAT hardware consists of a 4 Tesla superconductor system, an ultra high vacuum test section (vacuum approaching 10(exp -12) torr), and a high voltage confinement electrode system (up to 20 kvolts operation). The current laboratory layout is illustrated. The HiPAT designed objectives included storage of up to 1 trillion antiprotons with corresponding lifetimes approaching 18 days. To date, testing has centered on the storage of positive hydrogen ions produced in situ by a stream of high-energy electrons that passes through the trapping region. However, due to space charge issues and electron beam compression as it passes through the HiPAT central field, current ion production is limited to less then 50,000 ions. Ion lifetime was determined by counting particle populations at the end of various storage time intervals. Particle detection was accomplished by destructively expelling the ions against a micro-channel plate located just outside the traps magnetic field. The effect of radio frequency (RF) stabilization on the lifetime of trapped particles was also examined. This technique, referred to as a rotating wall, made use of a segmented electrode located near the center of the trap

  2. Expression of PAT and NPT II proteins during the developmental stages of a genetically modified pepper developed in Korea.

    PubMed

    Kim, Hyo Jin; Lee, Si Myung; Kim, Jae Kwang; Ryu, Tae Hun; Suh, Seok Cheol; Cho, Hyun Suk

    2010-10-27

    Estimation of the protein levels introduced in a biotechnology-derived product is conducted as part of an overall safety assessment. An enzyme-linked immunosorbent assay (ELISA) was used to analyze phosphinothricin acetyltransferase (PAT) and neomycin phosphotransferase II (NPT II) protein expression in a genetically modified (GM) pepper plant developed in Korea. PAT and NPT II expression levels, based on both dry weight and fresh weight, were variable among different plant generations and plant sections from isolated genetically modified organism (GMO) fields at four developmental stages. PAT expression was highest in leaves at anthesis (11.44 μg/gdw and 2.17 μg/gfw) and lowest in roots (0.12 μg/gdw and 0.01 μg/gfw). NPT II expression was also highest in leaves at anthesis (17.31 μg/gdw and 3.41 μg/gfw) and lowest in red pepper (0.65 μg/gdw and 0.12 μg/gfw). In pollen, PAT expression was 0.59-0.62 μg/gdw, while NPT II was not detected. Both PAT and NPT II showed a general pattern of decreased expression with progression of the growing season. As expected, PAT and NPT II protein expression was not detectable in control pepper plants. PMID:20873787

  3. The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms

    PubMed Central

    Jungfleisch, Jennifer; Chowdhury, Ashis; Alves-Rodrigues, Isabel; Tharun, Sundaresan; Díez, Juana

    2015-01-01

    The Lsm1-7-Pat1 complex binds to the 3′ end of cellular mRNAs and promotes 3′ end protection and 5′–3′ decay. Interestingly, this complex also specifically binds to cis-acting regulatory sequences of viral positive-strand RNA genomes promoting their translation and subsequent recruitment from translation to replication. Yet, how the Lsm1-7-Pat1 complex regulates these two processes remains elusive. Here, we show that Lsm1-7-Pat1 complex acts differentially in these processes. By using a collection of well-characterized lsm1 mutant alleles and a system that allows the replication of Brome mosaic virus (BMV) in yeast we show that the Lsm1-7-Pat1 complex integrity is essential for both, translation and recruitment. However, the intrinsic RNA-binding ability of the complex is only required for translation. Consistent with an RNA-binding-independent function of the Lsm1-7-Pat1 complex on BMV RNA recruitment, we show that the BMV 1a protein, the sole viral protein required for recruitment, interacts with this complex in an RNA-independent manner. Together, these results support a model wherein Lsm1-7-Pat1 complex binds consecutively to BMV RNA regulatory sequences and the 1a protein to promote viral RNA translation and later recruitment out of the host translation machinery to the viral replication complexes. PMID:26092942

  4. Performance characterization of low-cost, high-speed, portable pulsed laser diode photoacoustic tomography (PLD-PAT) system

    PubMed Central

    Upputuri, Paul Kumar; Pramanik, Manojit

    2015-01-01

    Photoacoustic tomography systems that uses Q-switched Nd:YAG/OPO pulsed lasers are expensive, bulky, and hence limits its use in clinical applications. The low pulse repetition rate of these lasers makes it unsuitable for real-time imaging when used with single-element ultrasound detector. In this work, we present a pulsed laser diode photoacoustic tomography (PLD-PAT) system that integrates a compact PLD inside a single-detector circular scanning geometry. We compared its performance against the traditional Nd:YAG/OPO based PAT system in terms of imaging depth, resolution, imaging time etc. The PLD provides near-infrared pulses at ~803 nm wavelength with pulse energy ~1.4 mJ/pulse at 7 kHz repetition rate. The PLD-PAT system is capable of providing 2D image in scan time as small as 3 sec with a signal-to-noise ratio ~30. High-speed and deep-tissue imaging is demonstrated on phantoms and biological samples. The PLD-PAT system is inexpensive, portable, allows high-speed PAT imaging, and its performance is as good as traditional expensive OPO based PAT system. Therefore, it holds promises for future translational biomedical imaging applications. PMID:26504659

  5. Transcriptomic analysis of tomato carpel development reveals alterations in ethylene and gibberellin synthesis during pat3/pat4 parthenocarpic fruit set

    PubMed Central

    Pascual, Laura; Blanca, Jose M; Cañizares, Joaquin; Nuez, Fernado

    2009-01-01

    Background Tomato fruit set is a key process that has a great economic impact on crop production. We employed the Affymetrix GeneChip Tomato Genome Array to compare the transcriptome of a non-parthenocarpic line, UC82, with that of the parthenocarpic line RP75/59 (pat3/pat4 mutant). We analyzed the transcriptome under normal conditions as well as with forced parthenocarpic development in RP75/59, emasculating the flowers 2 days before anthesis. This analysis helps to understand the fruit set in tomato. Results Differentially expressed genes were extracted with maSigPro, which is designed for the analysis of single and multiseries time course microarray experiments. 2842 genes showed changes throughout normal carpel development and fruit set. Most of them showed a change of expression at or after anthesis. The main differences between lines were concentrated at the anthesis stage. We found 758 genes differentially expressed in parthenocarpic fruit set. Among these genes we detected cell cycle-related genes that were still activated at anthesis in the parthenocarpic line, which shows the lack of arrest in the parthenocarpic line at anthesis. Key genes for the synthesis of gibberellins and ethylene, which were up-regulated in the parthenocarpic line were also detected. Conclusion Comparisons between array experiments determined that anthesis was the most different stage and the key point at which most of the genes were modulated. In the parthenocarpic line, anthesis seemed to be a short transitional stage to fruit set. In this line, the high GAs contends leads to the development of a parthenocarpic fruit, and ethylene may mimic pollination signals, inducing auxin synthesis in the ovary and the development of a jelly fruit. PMID:19480705

  6. Building on and Honoring Forty Years of PBL Scholarship from Howard Barrows: A Scientometric, Large-Scale Data, and Visualization-Based Analysis

    ERIC Educational Resources Information Center

    Xian, Hanjun; Madhavan, Krishna

    2013-01-01

    Over the past forty years, Howard Barrows' contributions to PBL research have influenced and guided educational research and practice in a diversity of domains. It is necessary to make visible to all PBL scholars what has been accomplished, what is perceived as significant, and what is the scope of applicability for Barrows' groundbreaking…

  7. The free radical scavenging and antioxidant activities of pod and seed extract of Clitoria fairchildiana (Howard)- an underutilized legume.

    PubMed

    Annegowda, H V; Bhat, Rajeev; Tze, Liong Min; Karim, A A; Mansor, S M

    2013-06-01

    We evaluated the phenolic content and antioxidant capacities of pod and seed extracts (in methanol, ethanol, and water) of an underutilized legume, Clitoria fairchildiana (Howard). The antioxidant capacity of the extracts was determined using the ferric reducing antioxidant potential assay, and the free radical-scavenging capacity was evaluated using 2,2-diphenyl-1-picrylhydrazyl radical-scavenging and ABTS assays. In addition, the total flavonoids, flavonols, and tannin contents were also determined. Overall, the methanol extracts of the pod contained high concentration of phenolics and showed high antioxidant capacities compared to seed extracts. In addition, a positive correlation was found between total phenol and tannin versus antioxidant capacity. Results of the present study indicate pods and seeds of C. fairchildiana to possess rich amount of natural antioxidants, and can be further explored for their possible use as a natural additive in food or in pharmaceutical industries. PMID:24425949

  8. A micromachined silicon parallel acoustic delay line (PADL) array for real-time photoacoustic tomography (PAT)

    NASA Astrophysics Data System (ADS)

    Cho, Young Y.; Chang, Cheng-Chung; Wang, Lihong V.; Zou, Jun

    2015-03-01

    To achieve real-time photoacoustic tomography (PAT), massive transducer arrays and data acquisition (DAQ) electronics are needed to receive the PA signals simultaneously, which results in complex and high-cost ultrasound receiver systems. To address this issue, we have developed a new PA data acquisition approach using acoustic time delay. Optical fibers were used as parallel acoustic delay lines (PADLs) to create different time delays in multiple channels of PA signals. This makes the PA signals reach a single-element transducer at different times. As a result, they can be properly received by single-channel DAQ electronics. However, due to their small diameter and fragility, using optical fiber as acoustic delay lines poses a number of challenges in the design, construction and packaging of the PADLs, thereby limiting their performances and use in real imaging applications. In this paper, we report the development of new silicon PADLs, which are directly made from silicon wafers using advanced micromachining technologies. The silicon PADLs have very low acoustic attenuation and distortion. A linear array of 16 silicon PADLs were assembled into a handheld package with one common input port and one common output port. To demonstrate its real-time PAT capability, the silicon PADL array (with its output port interfaced with a single-element transducer) was used to receive 16 channels of PA signals simultaneously from a tissue-mimicking optical phantom sample. The reconstructed PA image matches well with the imaging target. Therefore, the silicon PADL array can provide a 16× reduction in the ultrasound DAQ channels for real-time PAT.

  9. Dispersion and Transport of Cryptosporidium Oocysts from Fecal Pats under Simulated Rainfall Events

    PubMed Central

    Davies, Cheryl M.; Ferguson, Christobel M.; Kaucner, Christine; Krogh, Martin; Altavilla, Nanda; Deere, Daniel A.; Ashbolt, Nicholas J.

    2004-01-01

    The dispersion and initial transport of Cryptosporidium oocysts from fecal pats were investigated during artificial rainfall events on intact soil blocks (1,500 by 900 by 300 mm). Rainfall events of 55 mm h−1 for 30 min and 25 mm h−1 for 180 min were applied to soil plots with artificial fecal pats seeded with approximately 107 oocysts. The soil plots were divided in two, with one side devoid of vegetation and the other left with natural vegetation cover. Each combination of event intensity and duration, vegetation status, and degree of slope (5° and 10°) was evaluated twice. Generally, a fivefold increase (P < 0.05) in runoff volume was generated on bare soil compared to vegetated soil, and significantly more infiltration, although highly variable, occurred through the vegetated soil blocks (P < 0.05). Runoff volume, event conditions (intensity and duration), vegetation status, degree of slope, and their interactions significantly affected the load of oocysts in the runoff. Surface runoff transported from 100.2 oocysts from vegetated loam soil (25-mm h−1, 180-min event on 10° slope) to up to 104.5 oocysts from unvegetated soil (55-mm h−1, 30-min event on 10° slope) over a 1-m distance. Surface soil samples downhill of the fecal pat contained significantly higher concentrations of oocysts on devegetated blocks than on vegetated blocks. Based on these results, there is a need to account for surface soil vegetation coverage as well as slope and rainfall runoff in future assessments of Cryptosporidium transport and when managing pathogen loads from stock grazing near streams within drinking water watersheds. PMID:14766600

  10. Stacking Multiple Ion Captures in The High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Sims, William H.; Chakrabarti, Suman; Pearson, Boise; Fant, Wallace E.

    2004-01-01

    The High performance Antiproton Trap (HiPAT) research project was initiated by the Marshall Space Flight Center's propulsion Research Center to examining the fundamental behavior of low energy antiprotons. Stored antiproton would ultimately be used for experimental demonstration of basic propulsive concepts. Matter-antimatter annihilation produces approximately 10(exp 8) MJ/g nearly 10 orders of magnitude more energy per unit mass than chemical based combustion, hence NASA's interest. To achieve containment, HiPAT utilizes a type of electromagnetic bottle know as a Penning trap positioned within an ultrahigh vacuum test section. Recently, the HiPAT hardware configuration has been enhanced to facilitate the capture of multiple normal matter ion burst. This endeavor is often referred to as "stacking" and used to increasing the number of captured particles. A prior normal matter experimental effort, successfully demonstrated the effectiveness of single burst capture. The stacking process is accomplished by manipulating the electric field generated by the confinement electrodes i.e. adjusting the well potential depth. These potential well values are initially configured to maximize the quantity of captured ions per burst; shallow wells with a depth of 100 volt or less (referenced to the incoming ion beam energy) are typically selected. Once captured, a cooling interval is required to reduce the energy of trapped particles below the lower extent of the "trap door" (or leading electrode) ion emitting potential. This is necessary such that a new burst of hot ions can be introduced while preventing those already inside from escaping. The cooling time is driven by a combination of mechanisms such as synchrotron radiation, background gas scattering, and resistive damping in a time scale on the order of minutes. A potential for reducing this hold period is to actively manipulate the electric field shape, using the power supply control system, to produce a deeper potential

  11. EARLY IMPACT MELTING AND SPACE EXPOSURE HISTORY OF THE PAT91501 LCHONDRITE

    NASA Technical Reports Server (NTRS)

    Bogard, Donald D.; Garrison, D. H.; Herzog, G. F.; Xue, S.; Klein, J.; Middleton, R.

    2004-01-01

    Collisions probably occurred frequently in the early history of the asteroid belt. Their effects, which should be recorded in meteorites, must have included heating and melting along with shock alteration of mineral textures. Some non-chondritic meteorite types e.g., eucrites and IIE and IAB irons - do indeed give evidence of extensive impact heating more than 3.4 Gyr ago. The ordinary chondrites, in contrast, show little evidence of early impact heating. The Ar-Ar and Rb-Sr ages of ordinary chondrites that experienced intense shock are for the most part relatively young, many less than 1.5 Gyr. The numerous L-chondrites with Ar- Ar ages clustering near 0.5 Gy are a well-known example. One of them, the 105-kg Chico Lchondrite, shows the effects of unusually intense heating. It is approximately 60% impact melt and likely formed as a dyke beneath a large crater when the L-chondrite parent body underwent a very large impact approximately 0.5 Gyr ago. In rare instances, older shock dates are indicated for ordinary chondrites. Dixon et al show early impact resetting of Ar-Ar ages of a few LL-chondrites including MIL 99301 at 4.23 0.03 Gyr, but in none of these stones did shock lead to extensive melting. As of 2003, searches for chondritic melts attributable to early shock had turned up only the Shaw L-chondrite, which has an Ar-Ar age of approximately 4.42 Gyr. PAT91501 is an 8.55-kg L-chondrite containing vesicles and metal-troilite nodules. It is a unique, near-total impact melt, unshocked, depleted in siderophile and chalcophile elements, and contains only approximately 10% relic chondritic material. The authors conclude that PAT91501 crystallized rapidly and from a much more homogeneous melt than did Shaw. They suggest that PAT resembles Chico and likely formed as an impact melt vein within an impact crater. To define the history of PAT, we have determined its Ar-39-Ar-40 age and measured several radioactive and stable nuclides produced during its space exposure to

  12. Petrology and Geochemistry of LEW 88663 and PAT 91501: High Petrologic L Chondrites

    NASA Astrophysics Data System (ADS)

    Mittlefehldt, D. W.; Lindstrom, M. M.; Field, S. W.

    1993-07-01

    Primitive achondrites (e.g., Acapulco, Lodran) are believed to be highly metamorphosed chondritic materials, perhaps up to the point of anatexis in some types. Low petrologic grade equivalents of these achondrites are unknown, so the petrologic transition from chondritic to achondritic material cannot be documented. However, there are rare L chondrites of petrologic grade 7 that may have experienced igneous processes, and study of these may yield information relevant to the formation of primitive achondrites, and perhaps basaltic achondrites, from chondritic precursors. We have begun the study of the L7 chondrites LEW 88663 and PAT 91501 as part of our broader study of primitive achondrites. Here, we present our preliminary petrologic and geochemical data on these meteorites. Petrology and Mineral Compositions: LEW 88663 is a granular achondrite composed of equant, subhedral to anhedral olivine grains poikilitically enclosed in networks of orthopyroxene and plagioclase. Small grains of clinopyroxene are spatially associated with orthopyroxene. Troilite occurs as large anhedral and small rounded grains. The smaller troilite grains are associated with the orthopyroxene-plagioclase networks. PAT 91501 is a vesicular stone containing centimeter-sized troilite +/- metal nodules. Its texture consists of anhedral to euhedral olivine grains, anhedral orthopyroxene grains (some with euhedral clinopyroxene overgrowths), anhedral to euhedral clinopyroxene, and interstitial plagioclase and SiO2-Al2O3-K2O- rich glass. In some areas, olivine is poikilitically enclosed in orthopyroxene. Fine-grained troilite, metal, and euhedral chromite occur interstitial to the silicates. Average mineral compositions for LEW 88663 are olivine Fo(sub)75.8, orthopyroxene Wo(sub)3.4En(sub)76.2Fs(sub)20.4, clinopyroxene Wo(sub)42.6En(sub)47.8Fs(sub)9.6, plagioclase Ab(sub)75.0An(sub)21.6Or(sub)3.4. Mineral compositions for PAT 91501 are olivine Fo(sub)73.8, orthopyroxene Wo(sub)4.5En(sub)74.8Fs

  13. The Killer Will Remain Free: On Pat Parker and the Poetics of Madness.

    PubMed

    Ali, Kazim

    2015-01-01

    Poet and scholar Kazim Ali reads Pat Parker's Movement in Black intimately, one poet to another, uncovering the shadow-fact of the lives of most people of color: not only the anger that is somehow sublimated into every part of our lives but also the issue that carrying this feeling around has on our mental health itself-that "anger" and "madness" might have sources in one another. Ali concludes that Parker offers a brutal and clear-eyed and ultimately hopeful assessment of the conditions that were faced at the time, and even now, by communities of color. PMID:26075690

  14. The patA gene product, which contains a region similar to CheY of Escherichia coli, controls heterocyst pattern formation in the cyanobacterium Anabaena 7120.

    PubMed Central

    Liang, J; Scappino, L; Haselkorn, R

    1992-01-01

    In Anabaena 7120, heterocysts (cells specialized for nitrogen fixation) develop at the ends of filaments and at intervals within each filament. We have isolated a mutant Anabaena strain that develops heterocysts mostly at the ends of filaments. This mutant, PAT-1, grows poorly under nitrogen-fixing conditions. The wild-type gene that complements the mutation in PAT-1, called patA, was cloned and sequenced. The predicted PatA protein contains 379 amino acids distributed among three "domains" based on predictions of hydropathy and flexibility. The carboxyl-terminal domain is very similar to that of CheY and other response regulators in two-component regulatory systems in eubacteria. The patA mutation suppresses the multiheterocyst phenotype produced by extra copies of the wild-type hetR gene described previously, suggesting that PatA and HetR are components of the same environment-sensing regulatory circuit in Anabaena. Images PMID:1608976

  15. Numerical upper bounds on convective heat transport in a layer of fluid of finite Prandtl number: Confirmation of Howard's analytical asymptotic single-wave-number bound

    NASA Astrophysics Data System (ADS)

    Vitanov, Nikolay K.

    2005-10-01

    By means of the Howard-Busse method of the optimum theory of turbulence we investigate numerically the upper bounds on the Nusselt number in a heated-from-below horizontal layer of fluid of finite Prandtl number for the case of rigid boundaries. The bounds are obtained by the solutions of the Euler-Lagrange equations of a variational problem possessing up to three wave numbers. The obtained results are compared to the numerical results for the case of fluid layer with stress-free boundaries [N. K. Vitanov and F. H. Busse, "Upper bounds on heat transport in a horizontal fluid layer with stress-free boundaries," ZAMP 48, 310 (1997)] as well as to the numerical and analytical asymptotic results obtained by Howard ["Heat transport by turbulent convection," J. Fluid Mech. 17, 405 (1963)], Busse ["On Howard's upper bound for heat transport by turbulent convection," J. Fluid Mech. 37, 457 (1969)], and Strauss ["On the upper bounding approach to thermal convection at moderate Rayleigh numbers, II. Rigid boundaries," Dyn. Atm. Oceans 1, 77 (1976)]. We show that for low and intermediate Rayleigh numbers the numerical bounds are positioned below the analytical asymptotic bounds obtained by Howard and Busse. For large Rayleigh numbers the numerical bounds tend to approach the analytical asymptotic bounds. We confirm numerically the bound obtained by Howard for the case of one-wave-number solution of the Euler-Lagrange equations. As the region of validity of the results of the analytical asymptotic theory for solutions of the Euler-Lagrange equations with two and three wave numbers lies in the area of very high Rayleigh numbers the values of the second and third wave numbers are different from their analytical asymptotic values for the values of the Rayleigh number reached by the numerical computation.

  16. The utility of Pinus sylvestris L. in dendrochemical investigations: pollution impact of lead mining and smelting in Darley Dale, Derbyshire, UK.

    PubMed

    Lageard, J G A; Howell, J A; Rothwell, J J; Drew, I B

    2008-05-01

    This research investigates atmospheric pollution from an isolated and increasingly productive lead-smelting site by examining the dendrochemistry of Pinus sylvestris growing in the local environment and at control sites. Tree increment cores and soil in the rooting environment were analysed for lead content. Inter-site comparisons of lead-in-soil suggest that contamination of the soil may be a less important pathway for lead inclusion within wood than pathways via bark or needles. Levels of lead-in-wood (up to 38mgkg(-1)) are at the upper end of those previously reported. There is evidence of radial translocation of lead towards the heartwood and variability in intra-site dendrochemical records. Mean site lead-in-wood records can however be related to a well-documented pollution chronology and also suggest the importance of local topography in the dispersal and deposition of particulate lead. This study demonstrates that P. sylvestris can be used to estimate the scale and timing of past pollution episodes in similar environmental contexts to those investigated at Darley Dale, where precisely dated pollution chronologies are lacking. PMID:17959285

  17. Bridging the gap between PAT concepts and implementation: An integrated software platform for fermentation.

    PubMed

    Chopda, Viki R; Gomes, James; Rathore, Anurag S

    2016-01-01

    Bioreactor control significantly impacts both the amount and quality of the product being manufactured. The complexity of the control strategy that is implemented increases with reactor size, which may vary from thousands to tens of thousands of litres in commercial manufacturing. The Process Analytical Technology (PAT) initiative has highlighted the need for having robust monitoring tools and effective control schemes that are capable of taking real time information about the critical quality attributes (CQA) and the critical process parameters (CPP) and executing immediate response as soon as a deviation occurs. However, the limited flexibility that present commercial software packages offer creates a hurdle. Visual programming environments have gradually emerged as potential alternatives to the available text based languages. This paper showcases development of an integrated programme using a visual programming environment for a Sartorius BIOSTAT® B Plus 5L bioreactor through which various peripheral devices are interfaced. The proposed programme facilitates real-time access to data and allows for execution of control actions to follow the desired trajectory. Major benefits of such integrated software system include: (i) improved real time monitoring and control; (ii) reduced variability; (iii) improved performance; (iv) reduced operator-training time; (v) enhanced knowledge management; and (vi) easier PAT implementation. PMID:26647285

  18. Textural constraints on the formation of impact spherules: A case study from the Dales Gorge BIF, Paleoproterozoic Hamersley Group of Western Australia

    NASA Astrophysics Data System (ADS)

    Sweeney, D.; Simonson, B. M.

    2008-12-01

    Impact ejecta (about 2.5 Gyr old) in the DS4 layer of the Dales Gorge BIF (Hamersley Group, Western Australia) are so well preserved that many original textures such as vesicles and microlites are faithfully preserved. About 65% of the particles in the layer originated as impact ejecta, of which 81% are splash forms. The remaining 19% are angular, but the splash forms and angular particles have the same composition (mainly diagenetic stilpnomelane and K-feldspar) and share a common suite of internal textures. Some particles contain randomly oriented microlites texturally identical to plagioclase in basalts. Most splash forms have rims of inward-growing crystals that may have formed from the melt (perhaps nucleated by impinging dust) or via thermal devitrification. The rims clearly formed in flight because in broken particles (which make up about 13% of the splash forms) they are generally not present on broken surfaces. The origin of the angular particles is uncertain, but they may represent solid ejecta. Given the large sizes and variable shapes of the splash forms, they are probably droplets of impact melt emplaced ballistically. This is largely by analogy to the K-T boundary layer, but DS4 splash forms differ from K-T spherules in important ways suggesting the K-T model is not universal. The occurrence of basaltic ejecta from a large impact highlights its scarcity in the stratigraphic record despite the areal abundance of oceanic crust. The diverse textures formed via in-flight crystallization suggest particle paths in the plume are more complex than is generally appreciated.

  19. The Psychosocial Assessment Tool (PAT2.0): Psychometric Properties of a Screener for Psychosocial Distress in Families of Children Newly Diagnosed with Cancer

    PubMed Central

    Pai, Ahna L. H.; Patiño-Fernández, Anna Maria; McSherry, Mary; Beele, David; Alderfer, Melissa A.; Reilly, Anne T.; Hwang, Wei-Ting; Kazak, Anne E.

    2010-01-01

    Purpose Psychometric properties of the Psychosocial Assessment Tool 2.0 (PAT2.0), a brief screener for psychosocial risk in families of children with cancer, are presented. Methods Female (N =132) and male (N =72) caregivers of 141 children newly diagnosed with cancer completed the PAT2.0 and measures of child behavior symptoms, anxiety, acute stress, and family functioning to establish validity. Internal consistency and test–retest reliability of the PAT2.0 were also examined. Results Internal consistency and two-week test–retest for the PAT2.0 Total score was strong. Validity for the PAT2.0 was supported by significant correlations between the PAT2.0 subscales and measures of corresponding constructs. PAT2.0 Total scores were correlated with acute stress and child behavior symptoms for both mothers and fathers. Receiver-Operating Characteristic curves provided preliminary support for the proposed cutoffs. Conclusion The PAT2.0 Total score is a useful screening tool for family psychosocial risk in the pediatric oncology population. PMID:17609228

  20. PAT: From Western solid dosage forms to Chinese materia medica preparations using NIR-CI.

    PubMed

    Zhou, Luwei; Xu, Manfei; Wu, Zhisheng; Shi, Xinyuan; Qiao, Yanjiang

    2016-01-01

    Near-infrared chemical imaging (NIR-CI) is an emerging technology that combines traditional near-infrared spectroscopy with chemical imaging. Therefore, NIR-CI can extract spectral information from pharmaceutical products and simultaneously visualize the spatial distribution of chemical components. The rapid and non-destructive features of NIR-CI make it an attractive process analytical technology (PAT) for identifying and monitoring critical control parameters during the pharmaceutical manufacturing process. This review mainly focuses on the pharmaceutical applications of NIR-CI in each unit operation during the manufacturing processes, from the Western solid dosage forms to the Chinese materia medica preparations. Finally, future applications of chemical imaging in the pharmaceutical industry are discussed. Copyright © 2015 John Wiley & Sons, Ltd. PMID:25877484

  1. "Anything That Gets Me in My Heart": Pat Parker's Poetry of Justice.

    PubMed

    Green, David B

    2015-01-01

    This article argues that love and justice are interlocking themes that undergird and motivate the poetry and activism of the Black lesbian feminist Pat Parker. Parker was a prolific working-class poet, a committed Black lesbian feminist, and an international trailblazer whose poems, like her famous "Womanslaughter" discussed in this article, document the many injustices that Black women endured in an anti-Black, rabidly homophobic, and patriarchal U.S. during the last decades of the twentieth century. In a political moment where righteous cries of #BlackLivesMatter are heard across the United States I use this article to remind us all of the historical importance that Black lesbians played and continue to play in the struggles of anti-racist justice in America. PMID:26075686

  2. PAT1.1; Pinellas Action Tracking System; Tracks Audit Findings and Corrective Actions

    SciTech Connect

    Mellican, P.L.

    1993-04-09

    PAT was derived from a system that began at the Rocky Flats Plant and was further enhanced at the Mound Plant. Pinellas Plant obtained this system in 1990 to track Tiger Team Findings; it has been expanded to include new modules which encompass a wide range of related functions. Functionality includes tracking of findings and associated corrective actions from various sources such as line operations, self-assessments, oversight assessments, and external organizations. Other functionality includes Management Walk-About tracking, NEPA prioritization, Occurrence/incident Report corrective action tracking, and Management Action Item Tracking. The system utilizes state of the art relational database technology with pop-up windows for table lookups and entry of descriptive text. Standards such as assessment identification numbers, area designations, and finding category codes have been developed to provide enhanced query capabilities and the ability to group findings for trending purposes on a plant-wide basis.

  3. Principles and Practices Fostering Inclusive Excellence: Lessons from the Howard Hughes Medical Institute’s Capstone Institutions

    PubMed Central

    DiBartolo, Patricia Marten; Gregg-Jolly, Leslie; Gross, Deborah; Manduca, Cathryn A.; Iverson, Ellen; Cooke, David B.; Davis, Gregory K.; Davidson, Cameron; Hertz, Paul E.; Hibbard, Lisa; Ireland, Shubha K.; Mader, Catherine; Pai, Aditi; Raps, Shirley; Siwicki, Kathleen; Swartz, Jim E.

    2016-01-01

    Best-practices pedagogy in science, technology, engineering, and mathematics (STEM) aims for inclusive excellence that fosters student persistence. This paper describes principles of inclusivity across 11 primarily undergraduate institutions designated as Capstone Awardees in Howard Hughes Medical Institute’s (HHMI) 2012 competition. The Capstones represent a range of institutional missions, student profiles, and geographical locations. Each successfully directed activities toward persistence of STEM students, especially those from traditionally underrepresented groups, through a set of common elements: mentoring programs to build community; research experiences to strengthen scientific skill/identity; attention to quantitative skills; and outreach/bridge programs to broaden the student pool. This paper grounds these program elements in learning theory, emphasizing their essential principles with examples of how they were implemented within institutional contexts. We also describe common assessment approaches that in many cases informed programming and created traction for stakeholder buy-in. The lessons learned from our shared experiences in pursuit of inclusive excellence, including the resources housed on our companion website, can inform others’ efforts to increase access to and persistence in STEM in higher education. PMID:27562960

  4. Health-hazard evaluation report HETA-88-311-0000, Office of Disability Determination, The Howard Building, Tampa, Florida

    SciTech Connect

    Roper, P.; Lushniak, B.

    1989-01-01

    In response to a request from the Area Manager of the Office of Disability Determination, an evaluation was made of possible hazardous working conditions at the Howard Building, Tampa, Florida. Particular concern was voiced with regard to eye irritation, headaches, and fatigue with a possible connection to water leakage into the offices and pigeon nesting areas above suspended ceilings. Studies indicated no carbon-monoxide, nitrogen-dioxide, or formaldehyde concentration in the building. Carbon dioxide levels of 1000 parts per million or above were recorded which were indicative of inadequate air exchange between a building and the outdoors. An area was found along the south wall of the building with gross contamination by bird droppings and other debris. Rainwater leakage above the areas where the birds had nested washed the excrement and debris down over the office walls. As much as 2 inches of bird droppings were found on some suspended tiles. The crawl space above the first floor was observed to have health and safety hazards. Some of the symptoms were likely allergic rhinitis while others may have resulted from a lack of fresh outdoor air. Specific measures should be taken to safely remove the bird debris, prevent additional bird inhabitation, improve drainage of condensate water, and provide an outdoor air-supply system.

  5. The Preschool Attention-Deficit/Hyperactivity Disorder Treatment Study (PATS) 6-Year Follow-up

    PubMed Central

    Riddle, Mark A.; Yershova, Kseniya; Lazzaretto, Deborah; Paykina, Natalya; Yenokyan, Gayane; Greenhill, Laurence; Abikoff, Howard; Vitiello, Benedetto; Wigal, Tim; McCracken, James T.; Kollins, Scott H.; Murray, Desiree W.; Wigal, Sharon; Kastelic, Elizabeth; McGough, James J.; dosReis, Susan; Bauzó-Rosario, Audrey; Stehli, Annamarie; Posner, Kelly

    2013-01-01

    Objective To describe the clinical course of attention-deficit/hyperactivity disorder (ADHD) symptom severity and diagnosis from ages 3–5 to 9–12 years during a 6-year follow-up after the original Preschool ADHD Treatment Study (PATS). Method 207 participants (75% male) from the original PATS, assessed at Baseline (mean age 4.4 years, when all met criteria for ADHD) and 3-months later (prior to medication treatment), were re-evaluated in three follow-up assessment visits (Year 3, mean age 7.4 years; Year 4, 8.3 years and Year 6, 10.4 years). Parents and teachers rated symptom severity and clinicians established psychiatric diagnoses. Analyses examined longitudinal changes in symptom severity and ADHD diagnosis. Results Parent- and teacher-rated symptom severity decreased from Baseline to Year 3 but remained relatively stable and in the moderate-to-severe clinical range through Year 6. Girls showed generally steeper decreases in symptom T-scores. At Year 6, 89% (160/180) of remaining participants met ADHD symptom and impairment diagnostic criteria. Comorbidity of oppositional defiant disorder and/or conduct disorder was associated with a 30% higher risk of having an ADHD diagnosis at Year 6 in the multiple logistic model. Medication status during follow-up, on vs. off, did not predict symptom severity change from Year 3 to Year 6 after adjustment for other variables. Conclusions ADHD in preschoolers is a relatively stable diagnosis over a 6-year period. The course is generally chronic, with high symptom severity and impairment, in very young children with moderate-to-severe ADHD, despite treatment with medication. Development of more effective ADHD intervention strategies is needed for this age group. PMID:23452683

  6. RF Manipulation of Ions in the High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Pearson, J. Boise; Martin, James J.; Sims, William H.; Chakrabarti, Suman; Lewis, Raymond A.; Rodgers, Stephen L. (Technical Monitor)

    2002-01-01

    The annihilation of antimatter provides the highest mass specific energy of any other known reaction. Proper harnessing of this energy holds great promise for future space propulsion systems. Many different propulsion concepts have been proposed that take advantage of antimatter, either using matter-antimatter as the primary fuel, or as a 'spark plug' for fusion and fission systems. In order to begin to address these concepts experimentally, a method of storing and transporting antimatter must be developed. The High Performance Antiproton Trap (HiPAT) is a first-generation storage and transportation device designed to store and transport 10(exp 12) antiprotons with a storage half-life of 18 days. It uses a Penning-Malmberg ion trap with a 4T magnetic field and 20 kV potential. This will enable researchers much more flexibility in the design of antimatter experiments related to propulsion. Ions cannot be stored indefinitely in a real trap, as ion cloud instabilities develop from imperfections in manufacturing and misalignments in assembly. Previous work has been done at both the National Institute of Standards and University of California in San Diego in using RF (radio frequency) signals to both diagnose and confine the ion cloud. Two electrodes in the trap have been segmented to allow both reception and transmission of RF waves in the ion cloud. Experiments are underway to determine the number of ions and density in the cloud by "listening" to protons contained in the HiPAT. Currently we believe the density of ions stored in the trap is roughly 10(exp 15) m(exp -3). Development of non-destructive techniques is vital to the project goals, enabling continuous monitoring of the quantities stored in the system. Experimental work is also being done in identifying RF transmission frequencies that can manipulate the density of the cloud, by exchanging energy and momentum between the RF wave and the ions. Preliminary experiments have demonstrated this interaction.

  7. "The horizon opened up very greatly": Leland O. Howard and the transition to chemical insecticides in the United States, 1894-1927.

    PubMed

    McWilliams, James E

    2008-01-01

    The transition to synthetic chemicals as a popular method of insect control in the United States was one of the most critical developments in the history of American agriculture. Historians of agriculture have effectively identified the rise and charted the dominance of early chemical insecticides as they came to define commercial agriculture between the emergence of Paris green in the 1870s and the popularity of DDT in the 1940s and beyond. Less understood, however, are the underlying mechanics of this transition. this article thus takes up the basic question of how farmers and entomologists who were once dedicated to an impressively wide range of insect control options ultimately settled on the promise of a chemically driven approach to managing destructive insects. Central to this investigation is an emphasis on the bureaucratic maneuverings of Leland O. Howard, who headed the Bureau of Entomology from 1894 to 1927. Like most entomologists of his era, Howard was theoretically interested in pursuing a wide variety of control methods--biological, chemical, and cultural included. In the end, however, he employed several tactics to streamline the government's efforts to almost exclusively support arsenic and lead-based chemical insecticides as the most commercially viable form of insect control. While Howard in no way "caused" the national turn to chemicals, this article charts the pivotal role he played in fostering that outcome. PMID:19266680

  8. mini-PAT (Peer Assessment Tool): A Valid Component of a National Assessment Programme in the UK?

    ERIC Educational Resources Information Center

    Archer, Julian; Norcini, John; Southgate, Lesley; Heard, Shelley; Davies, Helena

    2008-01-01

    Purpose: To design, implement and evaluate a multisource feedback instrument to assess Foundation trainees across the UK. Methods: mini-PAT (Peer Assessment Tool) was modified from SPRAT (Sheffield Peer Review Assessment Tool), an established multisource feedback (360 [degree]) instrument to assess more senior doctors, as part of a blueprinting…

  9. CIO of the Year 2006: Pat Renzulli--The School District of Philadelphia's Top Technology Officer Puts Students First

    ERIC Educational Resources Information Center

    Technology & Learning, 2006

    2006-01-01

    This article profiles Pat Renzulli, CIO for the School District of Philadelphia, who was selected as David Kearns CIO of the Year 2006. Among other accomplishments, she developed a process for principals to receive key performance indicators, lead the implementation of an instructional management system, rolled out a district-wide technology…

  10. At-line process analytical technology (PAT) for more efficient scale up of biopharmaceutical microfiltration unit operations.

    PubMed

    Watson, Douglas S; Kerchner, Kristi R; Gant, Sean S; Pedersen, Joseph W; Hamburger, James B; Ortigosa, Allison D; Potgieter, Thomas I

    2016-01-01

    Tangential flow microfiltration (MF) is a cost-effective and robust bioprocess separation technique, but successful full scale implementation is hindered by the empirical, trial-and-error nature of scale-up. We present an integrated approach leveraging at-line process analytical technology (PAT) and mass balance based modeling to de-risk MF scale-up. Chromatography-based PAT was employed to improve the consistency of an MF step that had been a bottleneck in the process used to manufacture a therapeutic protein. A 10-min reverse phase ultra high performance liquid chromatography (RP-UPLC) assay was developed to provide at-line monitoring of protein concentration. The method was successfully validated and method performance was comparable to previously validated methods. The PAT tool revealed areas of divergence from a mass balance-based model, highlighting specific opportunities for process improvement. Adjustment of appropriate process controls led to improved operability and significantly increased yield, providing a successful example of PAT deployment in the downstream purification of a therapeutic protein. The general approach presented here should be broadly applicable to reduce risk during scale-up of filtration processes and should be suitable for feed-forward and feed-back process control. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 32:108-115, 2016. PMID:26519135

  11. Simultaneous binding of the anti-cancer IgM monoclonal antibody PAT-SM6 to low density lipoproteins and GRP78.

    PubMed

    Rosenes, Zachary; Mok, Yee-Foong; Yang, Shuo; Griffin, Michael D W; Mulhern, Terrence D; Hatters, Danny M; Hensel, Frank; Howlett, Geoffrey J

    2013-01-01

    The tumour-derived monoclonal IgM antibody PAT-SM6 specifically kills malignant cells by an apoptotic mechanism linked to the excessive uptake of plasma lipids. The mechanism is postulated to occur via the multi-point attachment of PAT-SM6 to the unfolded protein response regulator GRP78, located on the surface of tumour cells, coupled to the simultaneous binding of plasma low density lipoprotein (LDL). We prepared and characterised LDL and oxidized LDL using sedimentation velocity and small-angle X-ray scattering (SAXS) analysis. Enzyme-linked immunosorbent (ELISA) techniques indicated apparent dissociation constants of approximately 20 nM for the binding of LDL or oxidized LDL to PAT-SM6. ELISA experiments showed cross competition with LDL inhibiting PAT-SM6 binding to immobilised GRP78, while, in the reverse experiment, GRP78 inhibited PAT-SM6 binding to immobilized LDL. In contrast to the results of the ELISA experiments, sedimentation velocity experiments indicated relatively weak interactions between LDL and PAT-SM6, suggesting immunoabsorbance to the microtiter plate is driven by an avidity-based binding mechanism. The importance of avidity and the multipoint attachment of antigens to PAT-SM6 was further investigated using antigen-coated polystyrene beads. Absorption of GRP78 or LDL to polystyrene microspheres led to an increase in the inhibition of PAT-SM6 binding to microtiter plates coated with GRP78 or LDL, respectively. These results support the hypothesis that the biological action of PAT-SM6 in tumour cell apoptosis depends on the multivalent nature of PAT-SM6 and the ability to interact simultaneously with LDL and multiple GRP78 molecules clustered on the tumour cell surface. PMID:23620733

  12. BioPatRec: A modular research platform for the control of artificial limbs based on pattern recognition algorithms

    PubMed Central

    2013-01-01

    Background Processing and pattern recognition of myoelectric signals have been at the core of prosthetic control research in the last decade. Although most studies agree on reporting the accuracy of predicting predefined movements, there is a significant amount of study-dependent variables that make high-resolution inter-study comparison practically impossible. As an effort to provide a common research platform for the development and evaluation of algorithms in prosthetic control, we introduce BioPatRec as open source software. BioPatRec allows a seamless implementation of a variety of algorithms in the fields of (1) Signal processing; (2) Feature selection and extraction; (3) Pattern recognition; and, (4) Real-time control. Furthermore, since the platform is highly modular and customizable, researchers from different fields can seamlessly benchmark their algorithms by applying them in prosthetic control, without necessarily knowing how to obtain and process bioelectric signals, or how to produce and evaluate physically meaningful outputs. Results BioPatRec is demonstrated in this study by the implementation of a relatively new pattern recognition algorithm, namely Regulatory Feedback Networks (RFN). RFN produced comparable results to those of more sophisticated classifiers such as Linear Discriminant Analysis and Multi-Layer Perceptron. BioPatRec is released with these 3 fundamentally different classifiers, as well as all the necessary routines for the myoelectric control of a virtual hand; from data acquisition to real-time evaluations. All the required instructions for use and development are provided in the online project hosting platform, which includes issue tracking and an extensive “wiki”. This transparent implementation aims to facilitate collaboration and speed up utilization. Moreover, BioPatRec provides a publicly available repository of myoelectric signals that allow algorithms benchmarking on common data sets. This is particularly useful for

  13. The Catalytic Mechanism of the Marine-Derived Macrocyclase PatGmac.

    PubMed

    Brás, Natércia F; Ferreira, Pedro; Calixto, Ana R; Jaspars, Marcel; Houssen, Wael; Naismith, James H; Fernandes, Pedro A; Ramos, Maria J

    2016-09-01

    Cyclic peptides are a class of compounds with high therapeutic potential, possessing bioactivities including antitumor and antiviral (including anti-HIV). Despite their desirability, efficient design and production of these compounds has not been achieved to date. The catalytic mechanism of patellamide macrocyclization by the PatG macrocyclase domain has been computationally investigated by using quantum mechanics/molecular mechanics methodology, specifically ONIOM(M06/6-311++G(2d,2p):ff94//B3LYP/6-31G(d):ff94). The mechanism proposed herein begins with a proton transfer from Ser783 to His 618 and from the latter to Asp548. Nucleophilic attack of Ser783 on the substrate leads to the formation of an acyl-enzyme covalent complex. The leaving group Ala-Tyr-Asp-Gly (AYDG) of the substrate is protonated by the substrate's N terminus, leading to the breakage of the P1-P1' bond. Finally, the substrate's N terminus attacks the P1 residue, decomposing the acyl-enzyme complex forming the macrocycle. The formation and decomposition of the acyl-enzyme complex have the highest activation free energies (21.1 kcal mol(-1) and 19.8 kcal mol(-1) respectively), typical of serine proteases. Understanding the mechanism behind the macrocyclization of patellamides will be important to the application of the enzymes in the pharmaceutical and biotechnological industries. PMID:27389424

  14. A critical analysis of mini peer assessment tool (mini-PAT).

    PubMed

    Abdulla, Aza

    2008-01-01

    The structured evaluation of doctors' performance through peer review is a relatively new phenomenon brought about by public demand for accountability to patients. Medical knowledge (as assessed by examination score) is no longer a good predictor of individual performance, humanistic qualities and communication skills. The process of peer review (or multi-source assessment) was developed over the last two decades in the USA and has started to pick up momentum in the UK through the introduction of Modernizing Medical Careers. However the concept is not new. Driven by market forces, it was initially developed by industrial organizations to improve leadership qualities with a view to increasing productivity through positive behaviour change and self-awareness. Multi-source feedback is not without its problems and may not always produce its desired outcomes. In this article we review the evidence for peer review and critically discuss the current process of mini peer assessment tool (mini-PAT) as the assessment tool for peer review employed in UK. PMID:18263910

  15. Investigating 3-body Decays of Cluster States with the PAT-TPC

    NASA Astrophysics Data System (ADS)

    Carpenter, Lisa; Ayyad Limonge, Y.; Bazin, D.; Beceiro-Novo, S.; Bradt, J.; Cortesi, M.; Mittig, W.; Ahn, T.; Kolata, J. J.; Meisel, Z.; Bechetti, F. D.; Fritsch, A.; Howard, A.

    2016-03-01

    Recent model calculations with most advanced methods for cluster states have shown the need of experimental data to probe the structure of light exotic nuclei, including those with α-clustering, such as 14C. The Prototype Active Target Time Projection Chamber (PAT-TPC) allows us to investigate these types of structures, giving access to the full excitation function with a single beam energy. This type of experiment measures resonances in 14C that can be compared to the models. With an improved Micromegas pad plane with a circular backgammon design we are able to investigate 3-body decays in addition to 2-body scattering. The measurements were carried out by resonant alpha-scattering on 10Be beam delivered by the TwinSol facility at the University of Notre Dame. We also observed the 3-body decay of the Hoyle State in 12C from a 12N or 12B beam with the same device. Preliminary results will be presented. This work is supported by the National Science Foundation.

  16. Network and user interface for PAT DOME virtual motion environment system

    NASA Technical Reports Server (NTRS)

    Worthington, J. W.; Duncan, K. M.; Crosier, W. G.

    1993-01-01

    The Device for Orientation and Motion Environments Preflight Adaptation Trainer (DOME PAT) provides astronauts a virtual microgravity sensory environment designed to help alleviate tye symptoms of space motion sickness (SMS). The system consists of four microcomputers networked to provide real time control, and an image generator (IG) driving a wide angle video display inside a dome structure. The spherical display demands distortion correction. The system is currently being modified with a new graphical user interface (GUI) and a new Silicon Graphics IG. This paper will concentrate on the new GUI and the networking scheme. The new GUI eliminates proprietary graphics hardware and software, and instead makes use of standard and low cost PC video (CGA) and off the shelf software (Microsoft's Quick C). Mouse selection for user input is supported. The new Silicon Graphics IG requires an Ethernet interface. The microcomputer known as the Real Time Controller (RTC), which has overall control of the system and is written in Ada, was modified to use the free public domain NCSA Telnet software for Ethernet communications with the Silicon Graphics IG. The RTC also maintains the original ARCNET communications through Novell Netware IPX with the rest of the system. The Telnet TCP/IP protocol was first used for real-time communication, but because of buffering problems the Telnet datagram (UDP) protocol needed to be implemented. Since the Telnet modules are written in C, the Adap pragma 'Interface' was used to interface with the network calls.

  17. Ion Storage with the High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James; Lewis, Raymond; Chakrabarti, Suman; Pearson, Boise

    2002-01-01

    The matter antimatter reaction represents the densest form of energy storage/release known to modern physics: as such it offers one of the most compact sources of power for future deep space exploration. To take the first steps along this path, NASA-Marshall Space Flight Center is developing a storage system referred to as the High Performance Antiproton Trap (HiPAT) with a goal of maintaining 10(exp 12) particles for up to 18 days. Experiments have been performed with this hardware using normal matter (positive hydrogen ions) to assess the device's ability to hold charged particles. These ions are currently created using an electron gun method to ionize background gas; however, this technique is limited by the quantity that can be captured. To circumvent this issue, an ion source is currently being commissioned which will greatly increase the number of ions captured and more closely simulate actual operations expected at an antiproton production facility. Ions have been produced, stored for various time intervals, and then extracted against detectors to measure species, quantity and energy. Radio frequency stabilization has been tested as a method to prolong ion lifetime: results show an increase in the baseline 1/e lifetime of trapped particles from hours to days. Impurities in the residual background gas (typically carbon-containing species CH4, CO, CO2, etc.) present a continuing problem by reducing the trapped hydrogen population through the mechanism of ion charge exchange.

  18. Implementation of transmission NIR as a PAT tool for monitoring drug transformation during HME processing.

    PubMed

    Islam, Muhammad T; Scoutaris, Nikolaos; Maniruzzaman, Mohammed; Moradiya, Hiren G; Halsey, Sheelagh A; Bradley, Michael S A; Chowdhry, Babur Z; Snowden, Martin J; Douroumis, Dennis

    2015-10-01

    The aim of the work reported herein was to implement process analytical technology (PAT) tools during hot melt extrusion (HME) in order to obtain a better understanding of the relationship between HME processing parameters and the extruded formulations. For the first time two in-line NIR probes (transmission and reflectance) have been coupled with HME to monitor the extrusion of the water insoluble drug indomethacin (IND) in the presence of Soluplus (SOL) or Kollidon VA64 hydrophilic polymers. In-line extrusion monitoring of sheets, produced via a specially designed die, was conducted at various drug/polymer ratios and processing parameters. Characterisation of the extruded transparent sheets was also undertaken by using DSC, XRPD and Raman mapping. Analysis of the experimental findings revealed the production of molecular solutions where IND is homogeneously blended (ascertained by Raman mapping) in the polymer matrices, as it acts as a plasticizer for both hydrophilic polymers. PCA analysis of the recorded NIR signals showed that the screw speed used in HME affects the recorded spectra but not the homogeneity of the embedded drug in the polymer sheets. The IND/VA64 and IND/SOL extruded sheets displayed rapid dissolution rates with 80% and 30% of the IND being released, respectively within the first 20min. PMID:26209124

  19. Inline monitoring and a PAT strategy for pharmaceutical hot melt extrusion.

    PubMed

    Wahl, Patrick R; Treffer, Daniel; Mohr, Stefan; Roblegg, Eva; Koscher, Gerold; Khinast, Johannes G

    2013-10-15

    Implementation of continuous manufacturing in the pharmaceutical industry requires tight process control. This study focuses on a PAT strategy for hot melt extrusion of vegetable calcium stearate (CaSt) as matrix carrier and paracetamol as active pharmaceutical ingredient (API). The extrusion was monitored using in-line near-infrared (NIR) spectroscopy. A NIR probe was located in the section between the extrusion screws and the die, using a novel design of the die channel. A chemometric model was developed based on premixes at defined concentrations and was implemented in SIPAT for real time API concentration monitoring. Subsequently, step experiments were performed for different API concentrations, screw speeds and screw designs. The predicted API concentration was in good agreement with the pre-set concentrations. The transition from one API plateau to another was a smooth curve due to the mixing behaviour of the extruder. The accuracy of the model was confirmed via offline HPLC analysis. The screw design was determined as the main influential factor on content uniformity (CU). Additionally the influence of multiple feeders had a significant impact on CU. The results demonstrate that in-line NIR measurements is a powerful tool for process development (e.g., mixing characterization), monitoring and further control strategies. PMID:23911343

  20. [The Patient Rights Act (PatRG)--Part 3: Burden of proof in liability questions and critical resumé].

    PubMed

    Parzeller, Markus; Zedler, Barbara

    2014-01-01

    The article deals with the new regulations in the German Civil Code (Bürgerliches Gesetzbuch - BGB), which came into effect in Germany as the Patient Rights Act (PatRG) on the 2/26/2013. In the third part, the burden of proof in liability questions (and 630h BGB) are discussed and critically analysed. In the discussion and a final resume points of criticism of the new law are pointed out. PMID:24683869

  1. Comprehensive clinical studies in 34 patients with molecularly defined UPD(14)pat and related conditions (Kagami–Ogata syndrome)

    PubMed Central

    Kagami, Masayo; Kurosawa, Kenji; Miyazaki, Osamu; Ishino, Fumitoshi; Matsuoka, Kentaro; Ogata, Tsutomu

    2015-01-01

    Paternal uniparental disomy 14 (UPD(14)pat) and epimutations and microdeletions affecting the maternally derived 14q32.2 imprinted region lead to a unique constellation of clinical features such as facial abnormalities, small bell-shaped thorax with a coat-hanger appearance of the ribs, abdominal wall defects, placentomegaly, and polyhydramnios. In this study, we performed comprehensive clinical studies in patients with UPD(14)pat (n=23), epimutations (n=5), and microdeletions (n=6), and revealed several notable findings. First, a unique facial appearance with full cheeks and a protruding philtrum and distinctive chest roentgenograms with increased coat-hanger angles to the ribs constituted the pathognomonic features from infancy through childhood. Second, birth size was well preserved, with a median birth length of ±0 SD (range, −1.7 to +3.0 SD) and a median birth weight of +2.3 SD (range, +0.1 to +8.8 SD). Third, developmental delay and/or intellectual disability was invariably present, with a median developmental/intellectual quotient of 55 (range, 29–70). Fourth, hepatoblastoma was identified in three infantile patients (8.8%), and histological examination in two patients showed a poorly differentiated embryonal hepatoblastoma with focal macrotrabecular lesions and well-differentiated hepatoblastoma, respectively. These findings suggest the necessity of an adequate support for developmental delay and periodical screening for hepatoblastoma in the affected patients, and some phenotypic overlap between UPD(14)pat and related conditions and Beckwith–Wiedemann syndrome. On the basis of our previous and present studies that have made a significant contribution to the clarification of underlying (epi)genetic factors and the definition of clinical findings, we propose the name ‘Kagami–Ogata syndrome' for UPD(14)pat and related conditions. PMID:25689926

  2. Comprehensive clinical studies in 34 patients with molecularly defined UPD(14)pat and related conditions (Kagami-Ogata syndrome).

    PubMed

    Kagami, Masayo; Kurosawa, Kenji; Miyazaki, Osamu; Ishino, Fumitoshi; Matsuoka, Kentaro; Ogata, Tsutomu

    2015-11-01

    Paternal uniparental disomy 14 (UPD(14)pat) and epimutations and microdeletions affecting the maternally derived 14q32.2 imprinted region lead to a unique constellation of clinical features such as facial abnormalities, small bell-shaped thorax with a coat-hanger appearance of the ribs, abdominal wall defects, placentomegaly, and polyhydramnios. In this study, we performed comprehensive clinical studies in patients with UPD(14)pat (n=23), epimutations (n=5), and microdeletions (n=6), and revealed several notable findings. First, a unique facial appearance with full cheeks and a protruding philtrum and distinctive chest roentgenograms with increased coat-hanger angles to the ribs constituted the pathognomonic features from infancy through childhood. Second, birth size was well preserved, with a median birth length of ±0 SD (range, -1.7 to +3.0 SD) and a median birth weight of +2.3 SD (range, +0.1 to +8.8 SD). Third, developmental delay and/or intellectual disability was invariably present, with a median developmental/intellectual quotient of 55 (range, 29-70). Fourth, hepatoblastoma was identified in three infantile patients (8.8%), and histological examination in two patients showed a poorly differentiated embryonal hepatoblastoma with focal macrotrabecular lesions and well-differentiated hepatoblastoma, respectively. These findings suggest the necessity of an adequate support for developmental delay and periodical screening for hepatoblastoma in the affected patients, and some phenotypic overlap between UPD(14)pat and related conditions and Beckwith-Wiedemann syndrome. On the basis of our previous and present studies that have made a significant contribution to the clarification of underlying (epi)genetic factors and the definition of clinical findings, we propose the name 'Kagami-Ogata syndrome' for UPD(14)pat and related conditions. PMID:25689926

  3. The structure of the cyanobactin domain of unknown function from PatG in the patellamide gene cluster

    SciTech Connect

    Mann, Greg; Koehnke, Jesko; Bent, Andrew F.; Graham, Rachael; Houssen, Wael; Jaspars, Marcel; Schwarz-Linek, Uli; Naismith, James H.

    2014-11-14

    The highly conserved domain of unknown function in the cyanobactin superfamily has a novel fold. The protein does not appear to bind the most plausible substrates, leaving questions as to its role. Patellamides are members of the cyanobactin family of ribosomally synthesized and post-translationally modified cyclic peptide natural products, many of which, including some patellamides, are biologically active. A detailed mechanistic understanding of the biosynthetic pathway would enable the construction of a biotechnological ‘toolkit’ to make novel analogues of patellamides that are not found in nature. All but two of the protein domains involved in patellamide biosynthesis have been characterized. The two domains of unknown function (DUFs) are homologous to each other and are found at the C-termini of the multi-domain proteins PatA and PatG. The domain sequence is found in all cyanobactin-biosynthetic pathways characterized to date, implying a functional role in cyanobactin biosynthesis. Here, the crystal structure of the PatG DUF domain is reported and its binding interactions with plausible substrates are investigated.

  4. PAT-seq: a method to study the integration of 3'-UTR dynamics with gene expression in the eukaryotic transcriptome.

    PubMed

    Harrison, Paul F; Powell, David R; Clancy, Jennifer L; Preiss, Thomas; Boag, Peter R; Traven, Ana; Seemann, Torsten; Beilharz, Traude H

    2015-08-01

    A major objective of systems biology is to quantitatively integrate multiple parameters from genome-wide measurements. To integrate gene expression with dynamics in poly(A) tail length and adenylation site, we developed a targeted next-generation sequencing approach, Poly(A)-Test RNA-sequencing. PAT-seq returns (i) digital gene expression, (ii) polyadenylation site/s, and (iii) the polyadenylation-state within and between eukaryotic transcriptomes. PAT-seq differs from previous 3' focused RNA-seq methods in that it depends strictly on 3' adenylation within total RNA samples and that the full-native poly(A) tail is included in the sequencing libraries. Here, total RNA samples from budding yeast cells were analyzed to identify the intersect between adenylation state and gene expression in response to loss of the major cytoplasmic deadenylase Ccr4. Furthermore, concordant changes to gene expression and adenylation-state were demonstrated in the classic Crabtree-Warburg metabolic shift. Because all polyadenylated RNA is interrogated by the approach, alternative adenylation sites, noncoding RNA and RNA-decay intermediates were also identified. Most important, the PAT-seq approach uses standard sequencing procedures, supports significant multiplexing, and thus replication and rigorous statistical analyses can for the first time be brought to the measure of 3'-UTR dynamics genome wide. PMID:26092945

  5. High-speed pre-clinical brain imaging using pulsed laser diode based photoacoustic tomography (PLD-PAT) system

    NASA Astrophysics Data System (ADS)

    Upputuri, Paul Kumar; Pramanik, Manojit

    2016-03-01

    Photoacoustic tomography (PAT) is a promising biomedical imaging modality for small animal imaging, breast cancer imaging, monitoring of vascularisation, tumor angiogenesis, blood oxygenation, total haemoglobin concentration etc. The existing PAT systems that uses Q-switched Nd:YAG and OPO nanosecond lasers have limitations in clinical applications because they are expensive, non-potable and not suitable for real-time imaging due to their low pulse repetition rate. Low-energy pulsed near-infrared diode laser which are low-cost, compact, and light-weight (<200 grams), can be used as an alternate. In this work, we present a photoacoustic tomography system with a pulsed laser diode (PLD) that can nanosecond pulses with pulse energy 1.3 mJ/pulse at ~803 nm wavelength and 7000 Hz repetition rate. The PLD is integrated inside a single-detector circular scanning geometric system. To verify the high speed imaging capabilities of the PLD-PAT system, we performed in vivo experimental results on small animal brain imaging using this system. The proposed system is portable, low-cost and can provide real-time imaging.

  6. Howard, Trudy Mulcahy

    ERIC Educational Resources Information Center

    Momentum, 1972

    1972-01-01

    The mystique says that the women will always be available whenever the Church needs them, but unless we wipe the stardust from our eyes, we will be faced with an alarming shortage of capable candidates for the few archdiocesan positions that are finally carved out for women to serve. (Author/CB)

  7. Proton-Assisted Amino Acid Transporter PAT1 Complexes with Rag GTPases and Activates TORC1 on Late Endosomal and Lysosomal Membranes

    PubMed Central

    Ögmundsdóttir, Margrét H.; Heublein, Sabine; Kazi, Shubana; Reynolds, Bruno; Visvalingam, Shivanthy M.; Shaw, Michael K.; Goberdhan, Deborah C. I.

    2012-01-01

    Mammalian Target of Rapamycin Complex 1 (mTORC1) is activated by growth factor-regulated phosphoinositide 3-kinase (PI3K)/Akt/Rheb signalling and extracellular amino acids (AAs) to promote growth and proliferation. These AAs induce translocation of mTOR to late endosomes and lysosomes (LELs), subsequent activation via mechanisms involving the presence of intralumenal AAs, and interaction between mTORC1 and a multiprotein assembly containing Rag GTPases and the heterotrimeric Ragulator complex. However, the mechanisms by which AAs control these different aspects of mTORC1 activation are not well understood. We have recently shown that intracellular Proton-assisted Amino acid Transporter 1 (PAT1)/SLC36A1 is an essential mediator of AA-dependent mTORC1 activation. Here we demonstrate in Human Embryonic Kidney (HEK-293) cells that PAT1 is primarily located on LELs, physically interacts with the Rag GTPases and is required for normal AA-dependent mTOR relocalisation. We also use the powerful in vivo genetic methodologies available in Drosophila to investigate the regulation of the PAT1/Rag/Ragulator complex. We show that GFP-tagged PATs reside at both the cell surface and LELs in vivo, mirroring PAT1 distribution in several normal mammalian cell types. Elevated PI3K/Akt/Rheb signalling increases intracellular levels of PATs and synergistically enhances PAT-induced growth via a mechanism requiring endocytosis. In light of the recent identification of the vacuolar H+-ATPase as another Rag-interacting component, we propose a model in which PATs function as part of an AA-sensing engine that drives mTORC1 activation from LEL compartments. PMID:22574197

  8. patGPCR: A Multitemplate Approach for Improving 3D Structure Prediction of Transmembrane Helices of G-Protein-Coupled Receptors

    PubMed Central

    Wu, Hongjie; Lü, Qiang; Quan, Lijun; Qian, Peide; Xia, Xiaoyan

    2013-01-01

    The structures of the seven transmembrane helices of G-protein-coupled receptors are critically involved in many aspects of these receptors, such as receptor stability, ligand docking, and molecular function. Most of the previous multitemplate approaches have built a “super” template with very little merging of aligned fragments from different templates. Here, we present a parallelized multitemplate approach, patGPCR, to predict the 3D structures of transmembrane helices of G-protein-coupled receptors. patGPCR, which employs a bundle-packing related energy function that extends on the RosettaMem energy, parallelizes eight pipelines for transmembrane helix refinement and exchanges the optimized helix structures from multiple templates. We have investigated the performance of patGPCR on a test set containing eight determined G-protein-coupled receptors. The results indicate that patGPCR improves the TM RMSD of the predicted models by 33.64% on average against a single-template method. Compared with other homology approaches, the best models for five of the eight targets built by patGPCR had a lower TM RMSD than that obtained from SWISS-MODEL; patGPCR also showed lower average TM RMSD than single-template and multiple-template MODELLER. PMID:23554839

  9. Pharmacotherapy of the Preschool ADHD Treatment Study (PATS) Children Growing Up

    PubMed Central

    Vitiello, Benedetto; Lazzaretto, Deborah; Yershova, Kseniya; Abikoff, Howard; Paykina, Natalya; McCracken, James T.; McGough, James J.; Kollins, Scott H.; Greenhill, Laurence L.; Wigal, Sharon; Wigal, Tim; Riddle, Mark A.

    2015-01-01

    Objective To describe the long-term psychopharmacological treatment of children first diagnosed with attention-deficit/hyperactivity disorder (ADHD) as preschoolers. Method In a systematic, prospective, naturalistic follow-up, 206 (68.0%) of the 303 children who participated in the Preschool ADHD Treatment Study (PATS) were reassessed 3 years (mean age 7.4 years), and 179 (59.1%), 6 years (mean age 10.4 years), after completion of the controlled study. Pharmacotherapy and clinical data were obtained from the parents. Pharmacotherapy was defined as use of a specific class of medication for at least 50% of the days in the previous 6 months. Results At year 3, 34.0% were on no pharmacotherapy, 41.3% were on stimulant monotherapy, 9.2% on atomoxetine, alone or with a stimulant, 8.3% on an antipsychotic, usually together with a stimulant, and the remaining 7.2% on other pharmacotherapy; overall, 65.0% were on an indicated ADHD medication. At year 6, 26.8% were on no pharmacotherapy, 40.2% were on stimulant monotherapy, 4.5% on atomoxetine, alone or with a stimulant, 13.4% on an antipsychotic, and 15.1% on other pharmacotherapy; overall, 70.9% were on an indicated ADHD medication. Antipsychotic treatment was associated with more comorbidity, in particular disruptive behavior disorders and pervasive development disorders, and a lower level of functioning. Conclusion The long-term pharmacotherapy of preschoolers with ADHD was heterogeneous. While stimulant medication continued to be used by most children, about 1 child in 4 was off medication, and about 1 in 10 was on an antipsychotic. PMID:26088659

  10. Photon activated therapy (PAT) using monochromatic Synchrotron x-rays and iron oxide nanoparticles in a mouse tumor model: feasibility study of PAT for the treatment of superficial malignancy

    PubMed Central

    2012-01-01

    Background X-rays are known to interact with metallic nanoparticles, producing photoelectric species as radiosensitizing effects, and have been exploited in vivo mainly with gold nanoparticles. The purpose of this study was to investigate the potential of sensitizing effect of iron oxide nanoparticles for photon activated therapy. Methods X-rays photon activated therapy (PAT) was studied by treating CT26 tumor cells and CT26 tumor-bearing mice loaded with 13-nm diameter FeO NP, and irradiating them at 7.1 keV near the Fe K-edge using synchrotron x-rays radiation. Survival of cells was determined by MTT assay, and tumor regression assay was performed for in vivo model experiment. The results of PAT treated groups were compared with x-rays alone control groups. Results A more significant reduction in viability and damage was observed in the FeO NP-treated irradiated cells, compared to the radiation alone group (p < 0.04). Injection of FeO NP (100 mg/kg) 30 min prior to irradiation elevated the tumor concentration of magnetite to 40 μg of Fe/g tissue, with a tumor-to-muscle ratio of 17.4. The group receiving FeO NP and radiation of 10 Gy showed 80% complete tumor regression (CTR) after 15–35 days and relapse-free survival for up to 6 months, compared to the control group, which showed growth retardation, resulting in 80% fatality. The group receiving radiation of 40 Gy showed 100% CTR in all cases irrespective of the presence of FeO NP, but CTR was achieved earlier in the PAT-treated group compared with the radiation alone group. Conclusions An iron oxide nanoparticle enhanced therapeutic effect with relatively low tissue concentration of iron and 10 Gy of monochromatic X-rays. Since 7.1 keV X-rays is attenuated very sharply in the tissue, FeO NP-PAT may have promise as a potent treatment option for superficial malignancies in the skin, like chest wall recurrence of breast cancer. PMID:23111059

  11. The Formation and Chronology of the PAT 91501 Impact-Melt L-Chondrite with Vesicle-Metal-Sulfide Assemblages

    NASA Technical Reports Server (NTRS)

    Benedix, G. K.; Ketcham, R. A.; Wilson, L.; McCoy, T. J.; Bogard, D. D.; Garrison, D. H.; Herzog, G. F.; Xue, S.; Klein, J.; Middleton, R.

    2007-01-01

    The L chondrite Patuxent Range (PAT) 41 91501 is an 8.5-kg unshocked, homogeneous, igneous-textured impact melt that cooled slowly compared to other meteoritic impact melts in a crater floor melt sheet or sub-crater dike. We conducted mineralogical and tomographic studies of previously unstudied mm- to cm-sized metal-sulfide-vesicle assemblages and chronologic studies of the silicate host. Metal-sulfide clasts constitute about 1 vol.%, comprise zoned taenite, troilite and pentlandite, and exhibit a consistent orientation between metal and sulfide and of metal-sulfide contacts. Vesicles make up approximately 2 vol.% and exhibit a similar orientation of long axes. Ar-39-Ar-40 measurements date the time of impact at 4.461 +/- 0.008 Gyr B.P. Cosmogenic noble gases and Be-10 and Al-2l activities suggest a pre-atmospheric radius of 40-60 cm and a cosmic ray exposure age of 25-29 Myr, similar to ages of a cluster of L chondrites. PAT 91501 dates the oldest known impact on the L chondrite parent body. The dominant vesicle-forming gas was S2 (approximately 15-20 ppm), which formed in equilibrium with impact-melted sulfides. The meteorite formed in an impact melt dike beneath a crater, as did other impact melted L chondrites, such as Chico. Cooling and solidification occurred over approximately 2 hours. During this time, approximately 90% of metal and sulfide segregated from the local melt. Remaining metal and sulfide grains oriented themselves in the local gravitational field, a feature nearly unique among meteorites. Many of these metal sulfide grains adhered to vesicles to form aggregates that may have been close to neutrally buoyant. These aggregates would have been carried upward with the residual melt, inhibiting further buoyancy-driven segregation. Although similar processes operated individually in other chondritic impact melts, their interaction produced the unique assemblage observed in PAT 91501.

  12. Review of the High Performance Antiproton Trap (HiPAT) Experiment at the Marshall Space Flight Center

    NASA Technical Reports Server (NTRS)

    Pearson, J. B.; Sims, Herb; Martin, James; Chakrabarti, Suman; Lewis, Raymond; Fant, Wallace

    2003-01-01

    The significant energy density of matter-antimatter annihilation is attractive to the designers of future space propulsion systems, with the potential to offer a highly compact source of power. Many propulsion concepts exist that could take advantage of matter-antimatter reactions, and current antiproton production rates are sufficient to support basic proof-of-principle evaluation of technology associated with antimatter- derived propulsion. One enabling technology for such experiments is portable storage of low energy antiprotons, allowing antiprotons to be trapped, stored, and transported for use at an experimental facility. To address this need, the Marshall Space Flight Center's Propulsion Research Center is developing a storage system referred to as the High Performance Antiproton Trap (HiPAT) with a design goal of containing 10(exp 12) particles for up to 18 days. The HiPAT makes use of an electromagnetic system (Penning- Malmberg design) consisting of a 4 Telsa superconductor, high voltage electrode structure, radio frequency (RF) network, and ultra high vacuum system. To evaluate the system normal matter sources (both electron guns and ion sources) are used to generate charged particles. The electron beams ionize gas within the trapping region producing ions in situ, whereas the ion sources produce the particles external to the trapping region and required dynamic capture. A wide range of experiments has been performed examining factors such as ion storage lifetimes, effect of RF energy on storage lifetime, and ability to routinely perform dynamic ion capture. Current efforts have been focused on improving the FW rotating wall system to permit longer storage times and non-destructive diagnostics of stored ions. Typical particle detection is performed by extracting trapped ions from HiPAT and destructively colliding them with a micro-channel plate detector (providing number and energy information). This improved RF system has been used to detect various

  13. Discharge diversion in the Patía River delta, the Colombian Pacific: Geomorphic and ecological consequences for mangrove ecosystems

    NASA Astrophysics Data System (ADS)

    Restrepo, Juan D.; Cantera, Jaime R.

    2013-10-01

    In the Patía River delta, the best-developed delta on the western margin of South America, a major water diversion started in 1972. The diversion of the Patía flow to the Sanquianga River, the latter a small stream draining internal lakes from the Pacific lowlands, shifted the active delta plain from the south to the north and changed the northern estuarine system into an active delta plain. The Sanquianga Mangrove National Park, a mangrove reserve measuring 800 km2, lies in this former estuary, where major hydrologic and sedimentation changes are occurring. Overall, major environmental consequences of this discharge diversion in terms of geomorphic changes along distributary channels and ecological impacts on mangrove ecosystems are evidenced by: (1) distributary channel accretion by operating processes such as sedimentation, overbank flow, increasing width of levees, sedimentation in crevasses, interdistributary channel fill, and colonization of pioneer mangrove; (2) freshening conditions in the Sanquianga distributary channel, a hydrologic change that has shifted the upper estuarine region (salinity <1%) downstream; (3) downstream advance of freshwater vegetation, which is invading channel banks in the lower and mixing estuarine zones; (4) die-off of approximately 5200 ha of mangrove near the delta apex at Bocas de Satinga, where the highest sediment accumulation rates occur; and (5) recurrent periods of mangrove defoliation due to a worm plague. Further analyses indicate strong mangrove erosion along transgressive barrier islands on the former delta plain. Here tectonic-induced subsidence, relative sea-level rise, and sediment starving conditions due to the channel diversion, are the main causes of the observed retreating conditions of mangrove communities. Our data also indicate that the Patía River has the highest sediment load (27 × 106 t yr-1) and basin-wide sediment yield (1500 t km-2 yr-1) on the west coast of South America. Erosion rates from the Pat

  14. A Scintillation Counter System Design To Detect Antiproton Annihilation using the High Performance Antiproton Trap(HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Stanojev, Boris

    2003-01-01

    The High Performance Antiproton Trap (HiPAT), a system designed to hold up to l0(exp 12) charge particles with a storage half-life of approximately 18 days, is a tool to support basic antimatter research. NASA's interest stems from the energy density represented by the annihilation of matter with antimatter, 10(exp 2)MJ/g. The HiPAT is configured with a Penning-Malmberg style electromagnetic confinement region with field strengths up to 4 Tesla, and 20kV. To date a series of normal matter experiments, using positive and negative ions, have been performed evaluating the designs performance prior to operations with antiprotons. The primary methods of detecting and monitoring stored normal matter ions and antiprotons within the trap includes a destructive extraction technique that makes use of a micro channel plate (MCP) device and a non-destractive radio frequency scheme tuned to key particle frequencies. However, an independent means of detecting stored antiprotons is possible by making use of the actual annihilation products as a unique indicator. The immediate yield of the annihilation event includes photons and pie mesons, emanating spherically from the point of annihilation. To "count" these events, a hardware system of scintillators, discriminators, coincident meters and multi channel scalars (MCS) have been configured to surround much of the HiPAT. Signal coincidence with voting logic is an essential part of this system, necessary to weed out the single cosmic ray events from the multi-particle annihilation shower. This system can be operated in a variety of modes accommodating various conditions. The first is a low-speed sampling interval that monitors the background loss or "evaporation" rate of antiprotons held in the trap during long storage periods; provides an independent method of validating particle lifetimes. The second is a high-speed sample rate accumulating information on a microseconds time-scale; useful when trapped antiparticles are extracted

  15. PAT4 levels control amino-acid sensitivity of rapamycin-resistant mTORC1 from the Golgi and affect clinical outcome in colorectal cancer

    PubMed Central

    Fan, S-J; Snell, C; Turley, H; Li, J-L; McCormick, R; Perera, S M W; Heublein, S; Kazi, S; Azad, A; Wilson, C; Harris, A L; Goberdhan, D C I

    2016-01-01

    Tumour cells can use strategies that make them resistant to nutrient deprivation to outcompete their neighbours. A key integrator of the cell's responses to starvation and other stresses is amino-acid-dependent mechanistic target of rapamycin complex 1 (mTORC1). Activation of mTORC1 on late endosomes and lysosomes is facilitated by amino-acid transporters within the solute-linked carrier 36 (SLC36) and SLC38 families. Here, we analyse the functions of SLC36 family member, SLC36A4, otherwise known as proton-assisted amino-acid transporter 4 (PAT4), in colorectal cancer. We show that independent of other major pathological factors, high PAT4 expression is associated with reduced relapse-free survival after colorectal cancer surgery. Consistent with this, PAT4 promotes HCT116 human colorectal cancer cell proliferation in culture and tumour growth in xenograft models. Inducible knockdown in HCT116 cells reveals that PAT4 regulates a form of mTORC1 with two distinct properties: first, it preferentially targets eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1), and second, it is resistant to rapamycin treatment. Furthermore, in HCT116 cells two non-essential amino acids, glutamine and serine, which are often rapidly metabolised by tumour cells, regulate rapamycin-resistant mTORC1 in a PAT4-dependent manner. Overexpressed PAT4 is also able to promote rapamycin resistance in human embryonic kidney-293 cells. PAT4 is predominantly associated with the Golgi apparatus in a range of cell types, and in situ proximity ligation analysis shows that PAT4 interacts with both mTORC1 and its regulator Rab1A on the Golgi. These findings, together with other studies, suggest that differentially localised intracellular amino-acid transporters contribute to the activation of alternate forms of mTORC1. Furthermore, our data predict that colorectal cancer cells with high PAT4 expression will be more resistant to depletion of serine and glutamine, allowing them to

  16. PAT4 levels control amino-acid sensitivity of rapamycin-resistant mTORC1 from the Golgi and affect clinical outcome in colorectal cancer.

    PubMed

    Fan, S-J; Snell, C; Turley, H; Li, J-L; McCormick, R; Perera, S M W; Heublein, S; Kazi, S; Azad, A; Wilson, C; Harris, A L; Goberdhan, D C I

    2016-06-01

    Tumour cells can use strategies that make them resistant to nutrient deprivation to outcompete their neighbours. A key integrator of the cell's responses to starvation and other stresses is amino-acid-dependent mechanistic target of rapamycin complex 1 (mTORC1). Activation of mTORC1 on late endosomes and lysosomes is facilitated by amino-acid transporters within the solute-linked carrier 36 (SLC36) and SLC38 families. Here, we analyse the functions of SLC36 family member, SLC36A4, otherwise known as proton-assisted amino-acid transporter 4 (PAT4), in colorectal cancer. We show that independent of other major pathological factors, high PAT4 expression is associated with reduced relapse-free survival after colorectal cancer surgery. Consistent with this, PAT4 promotes HCT116 human colorectal cancer cell proliferation in culture and tumour growth in xenograft models. Inducible knockdown in HCT116 cells reveals that PAT4 regulates a form of mTORC1 with two distinct properties: first, it preferentially targets eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1), and second, it is resistant to rapamycin treatment. Furthermore, in HCT116 cells two non-essential amino acids, glutamine and serine, which are often rapidly metabolised by tumour cells, regulate rapamycin-resistant mTORC1 in a PAT4-dependent manner. Overexpressed PAT4 is also able to promote rapamycin resistance in human embryonic kidney-293 cells. PAT4 is predominantly associated with the Golgi apparatus in a range of cell types, and in situ proximity ligation analysis shows that PAT4 interacts with both mTORC1 and its regulator Rab1A on the Golgi. These findings, together with other studies, suggest that differentially localised intracellular amino-acid transporters contribute to the activation of alternate forms of mTORC1. Furthermore, our data predict that colorectal cancer cells with high PAT4 expression will be more resistant to depletion of serine and glutamine, allowing them to

  17. The orally active antihyperglycemic drug beta-guanidinopropionic acid is transported by the human proton-coupled amino acid transporter hPAT1.

    PubMed

    Metzner, Linda; Dorn, Madlen; Markwardt, Fritz; Brandsch, Matthias

    2009-01-01

    The orally administered creatine analogue beta-guanidinopropionic acid (beta-GPA) decreases plasma glucose levels by increasing the sensitivity to insulin. This effect is based on a beta-GPA induced expression of mRNA and total protein content of the insulin-responsive glucose transporter GLUT4. Although the oral availability of beta-GPA is well established, the underlying uptake mechanism has not yet been studied. We investigated whether the H(+)-coupled amino acid transporter PAT1, which is expressed in the apical membrane of intestinal cells, accepts guanidine derivatives as substrates. Uptake of l-[(3)H]proline into Caco-2 cells expressing hPAT1 constitutively was strongly inhibited by beta-GPA and its derivatives guanidinoacetic acid (GAA) and 4-guanidinobutyric acid (4-GBA). Competition assays revealed apparent affinity constants of about 1.5 mM. Electrophysiological measurements at hPAT1-expressing Xenopus laevis oocytes unequivocally demonstrated that beta-GPA, GAA and 4-GBA are effectively transported by this transport system in an electrogenic manner. We conclude that hPAT1 might be responsible for the intestinal absorption of beta-GPA thereby allowing its oral administration. Moreover, with beta-GPA we identified a new high affinity hPAT1 substrate that might be an interesting starting point for future drug design-drug delivery strategies. PMID:19358571

  18. The Rising Cost of Higher Education: Charles Eliot's "Free Money" Strategy and the Beginning of Howard Bowen's "Revenue Theory of Cost," 1869-1979

    ERIC Educational Resources Information Center

    Kimball, Bruce A.

    2014-01-01

    In order to explain the rising cost of higher education, economist Howard Bowen in 1980 proposed his "famous law" of institutional finance. Bowen based his "revenue theory of cost" on a study of aggregate quantitative data extending from 1929 to 1979. Neither he nor subsequent economists asked whether or how that…

  19. Mass-spectrometry analysis of histone post-translational modifications in pathology tissue using the PAT-H-MS approach.

    PubMed

    Noberini, Roberta; Pruneri, Giancarlo; Minucci, Saverio; Bonaldi, Tiziana

    2016-06-01

    Aberrant histone post-translational modifications (hPTMs) have been implicated with various pathologies, including cancer, and may represent useful epigenetic biomarkers. The data described here provide a mass spectrometry-based quantitative analysis of hPTMs from formalin-fixed paraffin-embedded (FFPE) tissues, from which histones were extracted through the recently developed PAT-H-MS method. First, we analyzed FFPE samples from mouse spleen and liver or human breast cancer up to six years old, together with their corresponding fresh frozen tissue. We then combined the PAT-H-MS approach with a histone-focused version of the super-SILAC strategy-using a mix of histones from four breast cancer cell lines as a spike-in standard- to accurately quantify hPTMs from breast cancer specimens belonging to different subtypes. The data, which are associated with a recent publication (Pathology tissue-quantitative mass spectrometry analysis to profile histone post-translational modification patterns in patient samples (Noberini, 2015) [1]), are deposited at the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD002669. PMID:27408908

  20. Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA

    PubMed Central

    Albesa-Jové, David; Svetlíková, Zuzana; Tersa, Montse; Sancho-Vaello, Enea; Carreras-González, Ana; Bonnet, Pascal; Arrasate, Pedro; Eguskiza, Ander; Angala, Shiva K.; Cifuente, Javier O.; Korduláková, Jana; Jackson, Mary; Mikušová, Katarína; Guerin, Marcelo E.

    2016-01-01

    The biosynthesis of phospholipids and glycolipids are critical pathways for virtually all cell membranes. PatA is an essential membrane associated acyltransferase involved in the biosynthesis of mycobacterial phosphatidyl-myo-inositol mannosides (PIMs). The enzyme transfers a palmitoyl moiety from palmitoyl–CoA to the 6-position of the mannose ring linked to 2-position of inositol in PIM1/PIM2. We report here the crystal structures of PatA from Mycobacterium smegmatis in the presence of its naturally occurring acyl donor palmitate and a nonhydrolyzable palmitoyl–CoA analog. The structures reveal an α/β architecture, with the acyl chain deeply buried into a hydrophobic pocket that runs perpendicular to a long groove where the active site is located. Enzyme catalysis is mediated by an unprecedented charge relay system, which markedly diverges from the canonical HX4D motif. Our studies establish the mechanistic basis of substrate/membrane recognition and catalysis for an important family of acyltransferases, providing exciting possibilities for inhibitor design. PMID:26965057

  1. Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA.

    PubMed

    Albesa-Jové, David; Svetlíková, Zuzana; Tersa, Montse; Sancho-Vaello, Enea; Carreras-González, Ana; Bonnet, Pascal; Arrasate, Pedro; Eguskiza, Ander; Angala, Shiva K; Cifuente, Javier O; Korduláková, Jana; Jackson, Mary; Mikušová, Katarína; Guerin, Marcelo E

    2016-01-01

    The biosynthesis of phospholipids and glycolipids are critical pathways for virtually all cell membranes. PatA is an essential membrane associated acyltransferase involved in the biosynthesis of mycobacterial phosphatidyl-myo-inositol mannosides (PIMs). The enzyme transfers a palmitoyl moiety from palmitoyl-CoA to the 6-position of the mannose ring linked to 2-position of inositol in PIM1/PIM2. We report here the crystal structures of PatA from Mycobacterium smegmatis in the presence of its naturally occurring acyl donor palmitate and a nonhydrolyzable palmitoyl-CoA analog. The structures reveal an α/β architecture, with the acyl chain deeply buried into a hydrophobic pocket that runs perpendicular to a long groove where the active site is located. Enzyme catalysis is mediated by an unprecedented charge relay system, which markedly diverges from the canonical HX4D motif. Our studies establish the mechanistic basis of substrate/membrane recognition and catalysis for an important family of acyltransferases, providing exciting possibilities for inhibitor design. PMID:26965057

  2. Small GTPase CDC-42 promotes apoptotic cell corpse clearance in response to PAT-2 and CED-1 in C. elegans

    PubMed Central

    Neukomm, L J; Zeng, S; Frei, A P; Huegli, P A; Hengartner, M O

    2014-01-01

    The rapid clearance of dying cells is important for the well-being of multicellular organisms. In C. elegans, cell corpse removal is mainly mediated by three parallel engulfment signaling cascades. These pathways include two small GTPases, MIG-2/RhoG and CED-10/Rac1. Here we present the identification and characterization of CDC-42 as a third GTPase involved in the regulation of cell corpse clearance. Genetic analyses performed by both loss of cdc-42 function and cdc-42 overexpression place cdc-42 in parallel to the ced-2/5/12 signaling module, in parallel to or upstream of the ced-10 module, and downstream of the ced-1/6/7 module. CDC-42 accumulates in engulfing cells at membranes surrounding apoptotic corpses. The formation of such halos depends on the integrins PAT-2/PAT-3, UNC-112 and the GEF protein UIG-1, but not on the canonical ced-1/6/7 or ced-2/5/12 signaling modules. Together, our results suggest that the small GTPase CDC-42 regulates apoptotic cell engulfment possibly upstream of the canonical Rac GTPase CED-10, by polarizing the engulfing cell toward the apoptotic corpse in response to integrin signaling and ced-1/6/7 signaling in C. elegans. PMID:24632947

  3. Asc-1, PAT2 and P2RX5 are novel cell surface markers for white, beige and brown adipocytes

    PubMed Central

    Ussar, Siegfried; Lee, Kevin Y.; Dankel, Simon N.; Boucher, Jeremie; Haering, Max-Felix; Kleinridders, Andre; Thomou, Thomas; Xue, Ruidan; Macotela, Yazmin; Cypess, Aaron M.; Tseng, Yu-Hua; Mellgren, Gunnar; Kahn, C. Ronald

    2015-01-01

    White, beige and brown adipocytes are developmentally and functionally distinct but often occur mixed together within individual depots. To target white, beige and brown adipocytes for diagnostic or therapeutic purposes, a better understanding of the cell surface properties of these cell types is essential. Using a combination of in silico, in vitro and in vivo methods, we have identified three new cell surface markers of adipose cell types. The amino acid transporter Asc-1 is a white adipocyte-specific cell surface protein, with little or no expression in brown adipocytes, whereas the amino acid transporter PAT2 and the purinergic receptor P2RX5 are cell surface markers expressed in classical brown and beige adipocytes in mice. These markers also selectively mark brown/beige and white adipocytes in human tissue. Thus, Asc-1, PAT2 and P2RX5 are membrane surface proteins that may serve as tools to identify and target white and brown/beige adipocytes for therapeutic purposes. PMID:25080478

  4. Human induced discharge diversion in a tropical delta and its environmental implications: The Patía River, Colombia

    NASA Astrophysics Data System (ADS)

    Restrepo, Juan D.; Kettner, Albert

    2012-03-01

    SummaryThe Patía River, the number one in terms of sediment yield ˜1500 t km-2 yr-1 draining the western South America, has the most extensive and well developed delta on the Pacific coast, measuring 1700 km2. During the Holocene, nature forced the Patía delta to the south; however, a major water diversion, starting in 1972, diverted the Patía flow to the Sanguianga River, the latter, a small stream draining internal lakes from the Pacific lowlands. This human induced discharge diversion shifted the active delta plain back to the north and changed the northern estuarine system into an active delta plain. Overall, major environmental consequences of this discharge diversion in terms of morphological changes along the delta coast and distributary channels, are evidenced by: (1) coastal retreat along the abandoned delta lobe; 63% of the southern shoreline is retreating at maximum rates of 7 m yr-1, with a corresponding coastal land loss of 106 m yr-1; (2) transgressive barrier islands with exposed peat soils in the surf zone; (3) abandonment of former active distributaries in the southern delta plain with associated closing of inlets and formation of ebb tidal deltas; (4) breaching events on barrier islands; and (5) distributary channel accretion in the northern delta plain by morphological processes such as sedimentation (also in crevasses), overbank flow, increasing width of levees, interdistributary channel fill, and colonization of pioneer mangrove. The Sanguianga Mangrove National Park (SMNP), the largest mangrove reserve in Colombia, measuring 800 km2, lies in this former estuary, where major hydrologic and sedimentation changes are occurring. Observed environmental changes in the SMNP, include (1) seaward advance of the sub-aqueous delta front at the Sanquianga inlet evidenced by an increase in tidal flat area from 5.4 Mm2 in 1986 to 14 Mm2 in 2001; (2) freshening conditions in the Sanguianga distributary channel, a hydrologic change that has shifted the

  5. The formation and chronology of the PAT 91501 impact-melt L chondrite with vesicle metal sulfide assemblages

    NASA Astrophysics Data System (ADS)

    Benedix, G. K.; Ketcham, R. A.; Wilson, L.; McCoy, T. J.; Bogard, D. D.; Garrison, D. H.; Herzog, G. F.; Xue, S.; Klein, J.; Middleton, R.

    2008-05-01

    The L chondrite Patuxent Range (PAT) 91501 is an 8.5-kg unshocked, homogeneous, igneous-textured impact melt that cooled slowly compared to other meteoritic impact melts in a crater floor melt sheet or sub-crater dike [Mittlefehldt D. W. and Lindstrom M. M. (2001) Petrology and geochemistry of Patuxent Range 91501 and Lewis Cliff 88663. Meteoritics Planet. Sci. 36, 439-457]. We conducted mineralogical and tomographic studies of previously unstudied mm- to cm-sized metal-sulfide-vesicle assemblages and chronologic studies of the silicate host. Metal-sulfide clasts constitute about 1 vol.%, comprise zoned taenite, troilite, and pentlandite, and exhibit a consistent orientation between metal and sulfide and of metal-sulfide contacts. Vesicles make up ˜2 vol.% and exhibit a similar orientation of long axes. 39Ar- 40Ar measurements probably date the time of impact at 4.461 ± 0.008 Gyr B.P. Cosmogenic noble gases and 10Be and 26Al activities suggest a pre-atmospheric radius of 40-60 cm and a cosmic ray exposure age of 25-29 Myr, similar to ages of a cluster of L chondrites. PAT 91501 dates the oldest known impact on the L chondrite parent body. The dominant vesicle-forming gas was S 2 (˜15-20 ppm), which formed in equilibrium with impact-melted sulfides. The meteorite formed in an impact melt dike beneath a crater, as did other impact melted L chondrites, such as Chico. Cooling and solidification occurred over ˜2 h. During this time, ˜90% of metal and sulfide segregated from the local melt. Remaining metal and sulfide grains oriented themselves in the local gravitational field, a feature nearly unique among meteorites. Many of these metal-sulfide grains adhered to vesicles to form aggregates that may have been close to neutrally buoyant. These aggregates would have been carried upward with the residual melt, inhibiting further buoyancy-driven segregation. Although similar processes operated individually in other chondritic impact melts, their interaction produced

  6. Patent information analysis methods and their effective use : A study through activities of PAT-LIST Research Workshop adviser

    NASA Astrophysics Data System (ADS)

    Nakamura, Sakae

    For effective use of technical information, various analytical tools and methods (e.g., patent map analysis) have been proposed. It was against this background that the “PAT-LIST Research Workshop” (supported by Raytec Co., Ltd.) was established in 2006. This article discusses, as an example, some actual research subject that the author as an adviser to the forum has studied through our activities in the past six years, especially the subject for 2010 (unveiling intellectual property strategies of specified enterprises from technical information analysis results). Practically useful analysis methods will be proposed showing some points of notes in analysis about the methods. What is also introduced is macroanalysis using text mining tools and the significance of controlled technical classification in a problem/solution map for determining critical fields.

  7. Strategic framework for education and training in Quality by Design (QbD) and process analytical technology (PAT).

    PubMed

    de Matas, Marcel; De Beer, Thomas; Folestad, Staffan; Ketolainen, Jarkko; Lindén, Hans; Lopes, João Almeida; Oostra, Wim; Weimer, Marco; Öhrngren, Per; Rantanen, Jukka

    2016-07-30

    The regulatory and technical landscape of the pharmaceutical field is rapidly evolving from one focused predominantly on development of small molecules, using well established manufacturing technologies towards an environment in which biologicals and complex modalities are being developed using advanced science and technology coupled with the application of modern Quality by Design (QbD) principles. In order that Europe keeps pace with these changes and sustains its position as major player in the development and commercialization of medicines, it is essential that measures are put in place to maintain a highly skilled workforce. A number of challenges however exist to equipping academic, industrial and health agency staff with the requisite knowledge, skills and experience to develop the next generation of medicines. In this regard, the EUFEPS QbD and PAT Sciences Network has proposed a structured framework for education, training and continued professional development, which comprises a number of pillars covering the fundamental principles of modern pharmaceutical development including the underpinning aspects of science, engineering and technology innovation. The framework is not prescriptive and is not aimed at describing specific course content in detail. It should however be used as a point of reference for those institutions delivering pharmaceutical based educational courses, to ensure that the necessary skills, knowledge and experience for successful pharmaceutical development are maintained. A positive start has been made and a number of examples of formal higher education courses and short training programs containing elements of this framework have been described. The ultimate vision for this framework however, is to see widespread adoption and proliferation of this curriculum with it forming the backbone of QbD and PAT science based skills development. PMID:27112991

  8. A Process Analytical Technology (PAT) approach to control a new API manufacturing process: development, validation and implementation.

    PubMed

    Schaefer, Cédric; Clicq, David; Lecomte, Clémence; Merschaert, Alain; Norrant, Edith; Fotiadu, Frédéric

    2014-03-01

    Pharmaceutical companies are progressively adopting and introducing Process Analytical Technology (PAT) and Quality-by-Design (QbD) concepts promoted by the regulatory agencies, aiming the building of the quality directly into the product by combining thorough scientific understanding and quality risk management. An analytical method based on near infrared (NIR) spectroscopy was developed as a PAT tool to control on-line an API (active pharmaceutical ingredient) manufacturing crystallization step during which the API and residual solvent contents need to be precisely determined to reach the predefined seeding point. An original methodology based on the QbD principles was designed to conduct the development and validation of the NIR method and to ensure that it is fitted for its intended use. On this basis, Partial least squares (PLS) models were developed and optimized using chemometrics methods. The method was fully validated according to the ICH Q2(R1) guideline and using the accuracy profile approach. The dosing ranges were evaluated to 9.0-12.0% w/w for the API and 0.18-1.50% w/w for the residual methanol. As by nature the variability of the sampling method and the reference method are included in the variability obtained for the NIR method during the validation phase, a real-time process monitoring exercise was performed to prove its fit for purpose. The implementation of this in-process control (IPC) method on the industrial plant from the launch of the new API synthesis process will enable automatic control of the final crystallization step in order to ensure a predefined quality level of the API. In addition, several valuable benefits are expected including reduction of the process time, suppression of a rather difficult sampling and tedious off-line analyses. PMID:24468350

  9. A double-focus collimator system for full PAT performance testing of inter-satellite laser communication terminals

    NASA Astrophysics Data System (ADS)

    Wang, Lijuan; Luan, Zhu; Sun, Jianfeng; Zhou, Yu; Liu, De'an; Liu, Liren

    2006-08-01

    A laser collimator is necessary for the testing and verification of the PAT performance of inter-satellite laser communication terminals. However, the terminals mostly have a large field of view for the acquisition and a high angular accuracy for the fine tracking needed to be examined. A single collimator has the conflict to reach at both a large field of view and a fine resolution. To compromise, a double-focus laser collimator is proposed. The collimator is mainly composed of a primary lens, a beam splitter, a secondary lens and some reflectors. The primary lens with a 9.9m focal length directly forms the long focal length arm of the collimator. The combination of the primary lens and the secondary lens has a new focal length of 1.3m and constructs the short focal length arm of the collimator. With two CMOS imaging sensors, the collimator can realize a 1.1mrad field of view with a <1μrad resolution in the focal plane of the long focal length arm and a 8.3mrad field of view with a 8.2μrad resolution in the focal plane of the short focal length arm. In combination with a coarse beam scanner (+/-15°) and a fine beam scanner (1mrad) to simulate the mutual angular movement between two satellites, the united system is capable to test the full PAT performance of inter-satellite laser communication terminals. The optical layouts of the collimator and two detecting units are illustrated. The optical design of the collimator is detailed. The mechanical design of the collimator is given.

  10. The Natural Human IgM Antibody PAT-SM6 Induces Apoptosis in Primary Human Multiple Myeloma Cells by Targeting Heat Shock Protein GRP78

    PubMed Central

    Rasche, Leo; Duell, Johannes; Morgner, Charlotte; Chatterjee, Manik; Hensel, Frank; Rosenwald, Andreas; Einsele, Hermann

    2013-01-01

    In contrast to other haematological malignancies, targeted immunotherapy has not entered standard treatment regimens for de novo or relapsed multiple myeloma (MM) yet. While a number of IgG-formatted monoclonal antibodies are currently being evaluated in clinical trials in MM, our study aimed to investigate whether the fully human IgM monoclonal antibody PAT-SM6 that targets a tumour-specific variant of the heat shock protein GRP78 might be an attractive candidate for future immunotherapeutic approaches. We here show that GRP78 is stably and consistently expressed on the surface on tumour cells from patients with de novo, but also relapsed MM and that binding of PAT-SM6 to MM cells can specifically exert cytotoxic effects on malignant plasma cells, whereas non-malignant cells are not targeted. We demonstrate that the induction of apoptosis and, to a lesser extent, complement dependent cytotoxicity is the main mode of action of PAT-SM6, whereas antibody dependent cellular cytotoxicity does not appear to contribute to the cytotoxic properties of this antibody. Given the favourable safety profile of PAT-SM6 in monkeys, but also in a recent phase I trial in patients with malignant melanoma, our results form the basis for a planned phase I study in patients with relapsed MM. PMID:23667612

  11. The natural human IgM antibody PAT-SM6 induces apoptosis in primary human multiple myeloma cells by targeting heat shock protein GRP78.

    PubMed

    Rasche, Leo; Duell, Johannes; Morgner, Charlotte; Chatterjee, Manik; Hensel, Frank; Rosenwald, Andreas; Einsele, Hermann; Topp, Max S; Brändlein, Stephanie

    2013-01-01

    In contrast to other haematological malignancies, targeted immunotherapy has not entered standard treatment regimens for de novo or relapsed multiple myeloma (MM) yet. While a number of IgG-formatted monoclonal antibodies are currently being evaluated in clinical trials in MM, our study aimed to investigate whether the fully human IgM monoclonal antibody PAT-SM6 that targets a tumour-specific variant of the heat shock protein GRP78 might be an attractive candidate for future immunotherapeutic approaches. We here show that GRP78 is stably and consistently expressed on the surface on tumour cells from patients with de novo, but also relapsed MM and that binding of PAT-SM6 to MM cells can specifically exert cytotoxic effects on malignant plasma cells, whereas non-malignant cells are not targeted. We demonstrate that the induction of apoptosis and, to a lesser extent, complement dependent cytotoxicity is the main mode of action of PAT-SM6, whereas antibody dependent cellular cytotoxicity does not appear to contribute to the cytotoxic properties of this antibody. Given the favourable safety profile of PAT-SM6 in monkeys, but also in a recent phase I trial in patients with malignant melanoma, our results form the basis for a planned phase I study in patients with relapsed MM. PMID:23667612

  12. A Tale of Two Melt Rocks: Equilibration and Metal/Sulfide-Silicate Segregation in the L7 Chondrites PAT 91501 and LEW 88663

    NASA Astrophysics Data System (ADS)

    Harvey, R. P.

    1993-07-01

    Type 7 ordinary chondrites have experienced temperatures near or beyond those necessary for partial melting. Two recently collected Antarctic specimens, PAT91501 (PAT) and LEW88663 (LEW), have been tentatively identified as L7 chondrites based on mineral and oxygen isotope compositions [1,2]. The petrology and mineralogy of these meteorites suggests that they have undergone significant metal/sulfide-silicate segregation, with implications for meteorite parent bodies. PAT consists of an equigranular contact-framework of nearly euhedral olivine grains, with interstitial spaces filled by plagioclase, pyroxenes, and several minor phases. Ortho- and clinopyroxene occur in an exsolution relationship. Olivine and pyroxene are highly equilibrated, varying <<1% in Fe-endmember content. Pyroxene equilibration temperatures calculated for PAT using the methods of [3] are self-consistent at about 1180 degrees C. In thin section, PAT contains only traces of metal, as tiny isolated blebs in sulfide grains; large (>1 cm) globular sulfide inclusions are seen in hand-sample [1], but are not present in the section examined. LEW was originally classified as an achondrite with olivine and pyroxene compositions similar to those in L chondrites [2]. Metal is absent in LEW, although the specimen is small and heavily rusted, making it impossible to gauge the original metal content. Olivine grains are commonly rounded in shape and seldom in contact with more than a few other grains. LEW olivine and pyroxene are also highly equilibrated. Veins of Ni-bearing metal oxides and sulfides are common. Both low- and high-Ca pyroxene occur as discrete grains, orthopyroxene often poikilitically enclosing olivine. Pyroxene equilibration temperatures for LEW are more variable than those for PAT and consistently lower, with an average around 900 degrees C. The various textural and compositional characteristics of PAT and LEW suggest they have experienced partial melting to varying degrees. Both visually

  13. GRP78-directed immunotherapy in relapsed or refractory multiple myeloma - results from a phase 1 trial with the monoclonal immunoglobulin M antibody PAT-SM6

    PubMed Central

    Rasche, Leo; Duell, Johannes; Castro, Inês C.; Dubljevic, Valentina; Chatterjee, Manik; Knop, Stefan; Hensel, Frank; Rosenwald, Andreas; Einsele, Hermann; Topp, Max S.; Brändlein, Stephanie

    2015-01-01

    The primary objective of this phase 1 study was to evaluate the safety and tolerability of the anti-glucose regulated protein 78 monoclonal immunoglobulin M antibody PAT-SM6 in subjects with relapsed or refractory multiple myeloma. Twelve heavily pretreated patients received four intravenous infusions of PAT-SM6 at doses of 0.3, 1, 3, and 6 mg/kg within 2 weeks. Efficacy, pharmacokinetics and immunogenicity were followed up until the end of the trial (day 36). In addition, immune cell patterns in peripheral blood were assessed by flow cytometry and glucose regulated protein 78 expression status was evaluated in bone marrow specimens by immunohistochemistry and flow cytometry at screening. All doses administered were found to be safe and well tolerated; the maximum tolerated dose was not reached. The most common treatment emergent adverse event was leukopenia (grades 1 and 2) in eight out of the 12 multiple myeloma patients. Pharmacokinetic analysis demonstrated dose-proportional increases in drug serum concentration. The terminal half-life ranged from 5.86 to 8.41 h, the apparent volume of distribution ranged from 101 to 150 mL/kg, and clearance ranged from 8.11 to 16.1 mL/h/kg. All patients showed glucose regulated protein 78 surface expression on multiple myeloma cells. Four out of the 12 patients (33.3 %) had stable disease, according to the International Myeloma Working Group criteria, after PAT-SM6 treatment across the doses 1, 3 and 6 mg/kg. In summary, single-agent PAT-SM6 was well tolerated with modest clinical activity in relapsed or refractory multiple myeloma. Further trials exploring the combination of PAT-SM6 with existing myeloma therapies are planned. Trial registration: clinicaltrials.gov identifier: NCT01727778 PMID:25637055

  14. Activation of hormone-sensitive lipase requires two steps, protein phosphorylation and binding to the PAT-1 domain of lipid droplet coat proteins.

    PubMed

    Wang, Hong; Hu, Liping; Dalen, Knut; Dorward, Heidi; Marcinkiewicz, Amy; Russell, Deanna; Gong, Dawei; Londos, Constantine; Yamaguchi, Tomohiro; Holm, Cecilia; Rizzo, Mark A; Brasaemle, Dawn; Sztalryd, Carole

    2009-11-13

    Lipolysis is an important metabolic pathway controlling energy homeostasis through degradation of triglycerides stored in lipid droplets and release of fatty acids. Lipid droplets of mammalian cells are coated with one or more members of the PAT protein family, which serve important functions in regulating lipolysis. In this study, we investigate the mechanisms by which PAT family members, perilipin A, adipose differentiation-related protein (ADFP), and LSDP5, control lipolysis catalyzed by hormone-sensitive lipase (HSL), a major lipase in adipocytes and several non-adipose cells. We applied fluorescence microscopic tools to analyze proteins in situ in cultured Chinese hamster ovary cells using fluorescence recovery after photobleaching and anisotropy Forster resonance energy transfer. Fluorescence recovery after photobleaching data show that ADFP and LSDP5 exchange between lipid droplet and cytoplasmic pools, whereas perilipin A does not. Differences in protein mobility do not correlate with PAT protein-mediated control of lipolysis catalyzed by HSL or endogenous lipases. Forster resonance energy transfer and co-immunoprecipitation experiments reveal that each of the three PAT proteins bind HSL through interaction of the lipase with amino acids within the highly conserved amino-terminal PAT-1 domain. ADFP and LSDP5 bind HSL under basal conditions, whereas phosphorylation of serine residues within three amino-terminal protein kinase A consensus sequences of perilipin A is required for HSL binding and maximal lipolysis. Finally, protein kinase A-mediated phosphorylation of HSL increases lipolysis in cells expressing ADFP or LSDP5; in contrast, phosphorylation of perilipin A exerts the major control over HSL-mediated lipolysis when perilipin is the main lipid droplet protein. PMID:19717842

  15. 'DIRTY WORK', BUT SOMEONE HAS TO DO IT: HOWARD P. ROBERTSON AND THE REFEREEING PRACTICES OF PHYSICAL REVIEW IN THE 1930S.

    PubMed

    Lalli, Roberto

    2016-06-20

    In the 1930s the mathematical physicist Howard P. Robertson was the main referee of the journal Physical Review for papers concerning general relativity and related subjects. The rich correspondence between Robertson and the editors of the journal enables a historical investigation of the refereeing process of Physical Review at the time that it was becoming one of the most influential physics periodicals in the world. By focusing on this case study, the paper investigates two complementary aspects of the evolution of the refereeing process: first, the historical evolution of the refereeing practices in connection with broader contextual changes, and second, the attempts to define the activity of the referee, including the epistemic virtues required and the journal's functions according to the participants' categories. By exploring the tension between Robertson's idealized picture about how the referee should behave and the desire to promote his intellectual agenda, I show that the evaluation criteria that Robertson employed were contextually dependent and I argue that, in the 1930s, through his reports the referee had an enormous power in defining what direction future research should take. PMID:27386715

  16. Revolutionary changes in medicine and ophthalmology--the St. Louis, MO, effect: Homer G. Phillips Hospital and Dr. Howard Phillip venable.

    PubMed Central

    Johnson, Andrew S.

    2003-01-01

    PURPOSE: To provide information on the revolutionary changes in medicine, particularly on the development of African-Americans in ophthalmology, created by Homer G. Phillips Hospital in St. Louis and the late Dr. Howard Phillip Venable. METHODS: Very little has been written about Homer G. Phillips Hospital and Dr. H. Phillip Venable. Through personal interviews with physicians trained by Dr. Venable and literature review, I was able to obtain information on Homer G. Phillips Hospital and Dr. Venable's influence in breaking the color line in medicine and ophthalmology. RESULTS: Homer G. Phillips Hospital in St. Louis was a major teaching hospital for African-American doctors. Dr. Venable was one of a few African-American ophthalmologists in the 1940s. Dr. Venable then trained approximately 40 African-American ophthalmologists at Homer G. Phillips Hospital between 1943 to 1979, when the hospital closed. CONCLUSION: The ophthalmologists trained by Dr. Venable have gone forward to treat thousands of patients with blinding eye diseases such as glaucoma and diabetes, and to influence others to become ophthalmologists. Although the hospital has closed and Dr. Venable has passed away, their existence has reformed the medical treatment received by African-Americans and other people. Images p484-a PMID:12856914

  17. Multi-parameter flow cytometry as a process analytical technology (PAT) approach for the assessment of bacterial ghost production.

    PubMed

    Langemann, Timo; Mayr, Ulrike Beate; Meitz, Andrea; Lubitz, Werner; Herwig, Christoph

    2016-01-01

    Flow cytometry (FCM) is a tool for the analysis of single-cell properties in a cell suspension. In this contribution, we present an improved FCM method for the assessment of E-lysis in Enterobacteriaceae. The result of the E-lysis process is empty bacterial envelopes-called bacterial ghosts (BGs)-that constitute potential products in the pharmaceutical field. BGs have reduced light scattering properties when compared with intact cells. In combination with viability information obtained from staining samples with the membrane potential-sensitive fluorescent dye bis-(1,3-dibutylarbituric acid) trimethine oxonol (DiBAC4(3)), the presented method allows to differentiate between populations of viable cells, dead cells, and BGs. Using a second fluorescent dye RH414 as a membrane marker, non-cellular background was excluded from the data which greatly improved the quality of the results. Using true volumetric absolute counting, the FCM data correlated well with cell count data obtained from colony-forming units (CFU) for viable populations. Applicability of the method to several Enterobacteriaceae (different Escherichia coli strains, Salmonella typhimurium, Shigella flexneri 2a) could be shown. The method was validated as a resilient process analytical technology (PAT) tool for the assessment of E-lysis and for particle counting during 20-l batch processes for the production of Escherichia coli Nissle 1917 BGs. PMID:26521248

  18. The conserved P body component HPat/Pat1 negatively regulates synaptic terminal growth at the larval Drosophila neuromuscular junction.

    PubMed

    Pradhan, Sarala J; Nesler, Katherine R; Rosen, Sarah F; Kato, Yasuko; Nakamura, Akira; Ramaswami, Mani; Barbee, Scott A

    2012-12-15

    The temporal and spatial regulation of protein synthesis plays an important role in the control of neural physiology. In axons and dendrites, translationally repressed mRNAs are actively transported to their destinations in a variety of ribonucleoprotein particles (RNPs). A subset of these neuronal RNPs has been shown to contain proteins associated with mRNA processing bodies (P bodies). P bodies are a class of highly conserved cytoplasmic granules that have been linked to both mRNA decay and translational repression via general and miRNA-mediated pathways. Here, we characterize functions for HPat/Pat1 (also known as Patr-1), a core component of P bodies, at the glutamatergic larval Drosophila neuromuscular junction (NMJ). We show that hpat mutants exhibit a strong synaptic hyperplasia at the NMJ. The synaptic defects observed in hpat mutants are associated with rearrangement of the axonal microtubule cytoskeleton suggesting that HPat negatively regulates presynaptic microtubule-based growth during NMJ development. Consistent with this, overexpression of HPat also blocks the rapid growth of presynaptic boutons induced by spaced depolarization. Finally, we demonstrate that HPat interacts genetically with the catalytic subunit of the deadenylase complex (twin/CCR4) and the miRNA pathway (Argonaute 1) to control bouton formation. We propose that HPat is required to target mRNAs involved in the control of microtubule architecture and synaptic terminal growth for repression, presumably in P bodies, via both general and miRNA-mediated mechanisms. PMID:23097047

  19. The conserved P body component HPat/Pat1 negatively regulates synaptic terminal growth at the larval Drosophila neuromuscular junction

    PubMed Central

    Pradhan, Sarala J.; Nesler, Katherine R.; Rosen, Sarah F.; Kato, Yasuko; Nakamura, Akira; Ramaswami, Mani; Barbee, Scott A.

    2012-01-01

    Summary The temporal and spatial regulation of protein synthesis plays an important role in the control of neural physiology. In axons and dendrites, translationally repressed mRNAs are actively transported to their destinations in a variety of ribonucleoprotein particles (RNPs). A subset of these neuronal RNPs has been shown to contain proteins associated with mRNA processing bodies (P bodies). P bodies are a class of highly conserved cytoplasmic granules that have been linked to both mRNA decay and translational repression via general and miRNA-mediated pathways. Here, we characterize functions for HPat/Pat1 (also known as Patr-1), a core component of P bodies, at the glutamatergic larval Drosophila neuromuscular junction (NMJ). We show that hpat mutants exhibit a strong synaptic hyperplasia at the NMJ. The synaptic defects observed in hpat mutants are associated with rearrangement of the axonal microtubule cytoskeleton suggesting that HPat negatively regulates presynaptic microtubule-based growth during NMJ development. Consistent with this, overexpression of HPat also blocks the rapid growth of presynaptic boutons induced by spaced depolarization. Finally, we demonstrate that HPat interacts genetically with the catalytic subunit of the deadenylase complex (twin/CCR4) and the miRNA pathway (Argonaute 1) to control bouton formation. We propose that HPat is required to target mRNAs involved in the control of microtubule architecture and synaptic terminal growth for repression, presumably in P bodies, via both general and miRNA-mediated mechanisms. PMID:23097047

  20. 3D noninvasive, high-resolution imaging using a photoacoustic tomography (PAT) system and rapid wavelength-cycling lasers

    NASA Astrophysics Data System (ADS)

    Sampathkumar, Ashwin; Gross, Daniel; Klosner, Marc; Chan, Gary; Wu, Chunbai; Heller, Donald F.

    2015-05-01

    Globally, cancer is a major health issue as advances in modern medicine continue to extend the human life span. Breast cancer ranks second as a cause of cancer death in women in the United States. Photoacoustic (PA) imaging (PAI) provides high molecular contrast at greater depths in tissue without the use of ionizing radiation. In this work, we describe the development of a PA tomography (PAT) system and a rapid wavelength-cycling Alexandrite laser designed for clinical PAI applications. The laser produces 450 mJ/pulse at 25 Hz to illuminate the entire breast, which eliminates the need to scan the laser source. Wavelength cycling provides a pulse sequence in which the output wavelength repeatedly alternates between 755 nm and 797 nm rapidly within milliseconds. We present imaging results of breast phantoms with inclusions of different sizes at varying depths, obtained with this laser source, a 5-MHz 128-element transducer and a 128-channel Verasonics system. Results include PA images and 3D reconstruction of the breast phantom at 755 and 797 nm, delineating the inclusions that mimic tumors in the breast.

  1. Re-Os systematics and age of pyrite associated with stratiform Zn-Pb mineralization in the Howards Pass district, Yukon and Northwest Territories, Canada

    NASA Astrophysics Data System (ADS)

    Kelley, Karen D.; Selby, David; Falck, Hendrik; Slack, John F.

    2016-05-01

    Stratiform Zn-Pb deposits hosted in unmetamorphosed carbonaceous and siliceous mudstones of the Ordovician to Silurian Duo Lake Formation define the Howards Pass district in Yukon Territory and Northwest Territories, western Canada. Collectively, the deposits are amongst the largest in the world, containing drill-indicated and inferred resources of 423 Mt at 4.84 % Zn and 1.59 % Pb. Sulphide textures include (a) fine-scale laminations of sphalerite, galena, and pyrite from <0.05 mm to 1 cm thick, interbedded with carbonaceous sedimentary rock; (b) layers of coarse sulphide that are structurally controlled by microfolds; and (c) veins that cut bedding and sulphide laminations. The finely interlaminated nature of sulphides with mudstone has been used as evidence for syngenetic mineralizing processes, whereas paleomagnetic data determined on coarse layered sulphides suggest a Middle Jurassic age of mineralization. Here, we present new rhenium-osmium (Re-Os) isotopic data for 12 pyrite separates obtained from 4 laminated sulphide-rich samples from the XY Central (XYC) and Don (DON) deposits and for 1 unmineralized organic-rich mudstone ˜20 m stratigraphically below the sulphide-bearing zone. Pyrite separates that lack mudstone inclusions ("pure") from the XYC deposit contain 2.2 to 4.0 ppb Re and 93.4 to 123.4 ppt Os; pure pyrite from the DON deposit is significantly more enriched in Re and Os (34-37 ppb Re; 636.8-694.9 ppt Os). The 187Re/188Os values of pure pyrite separates from the XYC and DON deposits range from 137.6 to 197 and 182.1 to 201.4, respectively. Regression of all pure pyrite Re-Os data from both deposits yields an isochron age of 442 ± 14 Ma (MSWD = 7.4) and an initial 187Os/188Os (Osi) value of 0.71 ± 0.07. The Re-Os age indicates that the early phase of pyrite precipitation (and by inference, sphalerite and galena) occurred during the early Silurian, consistent with biostratigraphic ages of the host rocks. The Osi value of ˜0.8 for earliest

  2. Development of a multivariate light-induced fluorescence (LIF) PAT tool for in-line quantitative analysis of pharmaceutical granules in a V-blender.

    PubMed

    Guay, Jean-Maxime; Lapointe-Garant, Pierre-Philippe; Gosselin, Ryan; Simard, Jean-Sébastien; Abatzoglou, Nicolas

    2014-04-01

    Process analytical technologies (PAT) enable process insight, process control and real-time testing. Light-induced fluorescence (LIF) spectroscopy is especially well suited for low-concentration ingredients as, in many cases, it is the most sensitive probe of the in-line PAT toolbox. This study is aimed at verifying the applicability of a multivariate LIF analyzer to monitor granulated powder blends in industrial settings. Its targets are to: (1) evaluate the critical parameters of powders to obtain robust, precise and accurate concentration predictions and (2) assess technology performance for in-line monitoring of blending operations. Varying dye properties, moisture levels and particle sizes have been shown to have the most significant impact on fluorescence emission. Reliable quantitative models can be obtained by controlling and/or mitigating these factors. PMID:24373731

  3. WAVES (Water Vapor Variability Satellite/Sondes) - An Aura Satellite Validation Field Campaign Hosted at the Howard University Research Campus in Beltsville, MD

    NASA Astrophysics Data System (ADS)

    Whiteman, D. N.; Adam, M.; Barnet, C.; Bojkov, B.; Demoz, B.; Fitzgibbon, J.; Forno, R.; Herman, R.; Hoff, R.; Joseph, E.; Landulfo, E.; McCann, K.; McGee, T.; Miloshevich, L.; Restrepo, I.; Schmidlin, F.; Taubman, B.; Thompson, A.; Venable, D.; Voemel, H.; Walthall, C.

    2006-12-01

    The NASA Aura satellite was launched on July 15, 2004 and is the trailing member of the A-train series of satellites. A field campaign called WAVES2006 (Water Vapor Variability Satellite-Sondes) was hosted at the Howard University Research Campus in Beltsville, MD from July 7 - August 10, 2006 to provide research quality measurements of ozone, temperature, pressure and water vapor for comparison with Aura satellite retrievals and for instrument accuracy assessment studies. Because of the small difference in overpass time, WAVES2006 measurements are also being used for Aqua satellite retrieval studies. The operations plan for WAVES included intensive observations by multiple radiosonde/ozonesonde sensors and several lidar systems during thirty-seven overpasses of the Aura satellite. Special staring-mode observations of the Beltsville region were performed by the TES (Tropospheric Emission Spectrometer) instrument during WAVES, which increased the amount of coincident data for evaluating an updated TES ozone retrieval algorithm. NOAA-NESDIS provided AIRS water vapor and ozone retrievals using both the near real-time system running the AIRS science team version 4 algorithm and a research algorithm incorporating the latest algorithm improvements planned for version 5. Coordinated measurements from 4 different lidar systems were acquired during WAVES. The Penn State University NATIVE trailer containing extensive gas sampling and radiation measurement instrumentation was also deployed to the Beltsville site for WAVES. The measurements from this system are being compared with comparable measurements from the MDE instrumentation adding to the confidence level of the trace gas validation data. The U.S. Department of Agriculture deployed a sun-photometer member of the AERONET network for use during WAVES. A member of the SuomiNet network of GPS systems was deployed by NASA-GSFC for total column water measurements. An overview of the WAVES campaign and preliminary comparison

  4. PAT-seq: a method to study the integration of 3′-UTR dynamics with gene expression in the eukaryotic transcriptome

    PubMed Central

    Harrison, Paul F.; Powell, David R.; Clancy, Jennifer L.; Preiss, Thomas; Boag, Peter R.; Traven, Ana; Seemann, Torsten; Beilharz, Traude H.

    2015-01-01

    A major objective of systems biology is to quantitatively integrate multiple parameters from genome-wide measurements. To integrate gene expression with dynamics in poly(A) tail length and adenylation site, we developed a targeted next-generation sequencing approach, Poly(A)-Test RNA-sequencing. PAT-seq returns (i) digital gene expression, (ii) polyadenylation site/s, and (iii) the polyadenylation-state within and between eukaryotic transcriptomes. PAT-seq differs from previous 3′ focused RNA-seq methods in that it depends strictly on 3′ adenylation within total RNA samples and that the full-native poly(A) tail is included in the sequencing libraries. Here, total RNA samples from budding yeast cells were analyzed to identify the intersect between adenylation state and gene expression in response to loss of the major cytoplasmic deadenylase Ccr4. Furthermore, concordant changes to gene expression and adenylation-state were demonstrated in the classic Crabtree–Warburg metabolic shift. Because all polyadenylated RNA is interrogated by the approach, alternative adenylation sites, noncoding RNA and RNA-decay intermediates were also identified. Most important, the PAT-seq approach uses standard sequencing procedures, supports significant multiplexing, and thus replication and rigorous statistical analyses can for the first time be brought to the measure of 3′-UTR dynamics genome wide. PMID:26092945

  5. Real-time determination of critical quality attributes using near-infrared spectroscopy: a contribution for Process Analytical Technology (PAT).

    PubMed

    Rosas, Juan G; Blanco, Marcel; González, Josep M; Alcalà, Manel

    2012-08-15

    Process Analytical Technology (PAT) is playing a central role in current regulations on pharmaceutical production processes. Proper understanding of all operations and variables connecting the raw materials to end products is one of the keys to ensuring quality of the products and continuous improvement in their production. Near infrared spectroscopy (NIRS) has been successfully used to develop faster and non-invasive quantitative methods for real-time predicting critical quality attributes (CQA) of pharmaceutical granulates (API content, pH, moisture, flowability, angle of repose and particle size). NIR spectra have been acquired from the bin blender after granulation process in a non-classified area without the need of sample withdrawal. The methodology used for data acquisition, calibration modelling and method application in this context is relatively inexpensive and can be easily implemented by most pharmaceutical laboratories. For this purpose, Partial Least-Squares (PLS) algorithm was used to calculate multivariate calibration models, that provided acceptable Root Mean Square Error of Predictions (RMSEP) values (RMSEP(API)=1.0 mg/g; RMSEP(pH)=0.1; RMSEP(Moisture)=0.1%; RMSEP(Flowability)=0.6 g/s; RMSEP(Angle of repose)=1.7° and RMSEP(Particle size)=2.5%) that allowed the application for routine analyses of production batches. The proposed method affords quality assessment of end products and the determination of important parameters with a view to understanding production processes used by the pharmaceutical industry. As shown here, the NIRS technique is a highly suitable tool for Process Analytical Technologies. PMID:22841062

  6. The world-class Howard's Pass SEDEX Zn-Pb district, Selwyn Basin, Yukon. Part II: the roles of thermochemical and bacterial sulfate reduction in metal fixation

    NASA Astrophysics Data System (ADS)

    Gadd, Michael G.; Layton-Matthews, Daniel; Peter, Jan M.; Paradis, Suzanne; Jonasson, Ian R.

    2016-07-01

    The Howard's Pass district of sedimentary exhalative (SEDEX) Zn-Pb deposits is located in Yukon Territory and comprises 14 Zn-Pb deposits that contain an estimated 400.7 Mt of sulfide mineralization grading 4.5 % Zn and 1.5 % Pb. Mineralization is hosted in carbonaceous and calcareous and, to a lesser extent, siliceous mudstones. Pyrite is a minor but ubiquitous mineral in the host rocks stratigraphically above, within, and below mineralization. Petrographic analyses reveal that pyrite has a complex and protracted growth history, preserving multiple generations of pyrite within single grains. Sulfur isotope analysis of paragenetically complex pyrite by secondary ion mass spectrometry (SIMS) reveals that sulfur isotope compositions vary with textural zonation. Within the Zn-Pb deposits, framboidal pyrite is the earliest pyrite generation recognized, and this exclusively has negative δ34S values (mean = -16.6 ± 4.1 ‰; n = 55), whereas paragenetically later pyrite and galena possess positive δ34S values (mean = 29.1 ± 7.5 and 22.4 ± 3.0 ‰, n = 13 and 13, respectively). Previous studies found that sphalerite and galena mineral separates have exclusively positive δ34S values (mean = 16.8 ± 3.3 and 12.7 ± 2.8 ‰, respectively; Goodfellow and Jonasson 1986). These distinct sulfur isotope values are interpreted to reflect varying contributions of bacterially reduced seawater sulfate (negative; framboidal pyrite) and thermochemically reduced seawater sulfate and/or hydrothermal sulfate (positive; galena, sphalerite, later forms of pyrite). Textural evidence indicates that framboidal pyrite predates galena and sphalerite deposition. Collectively, the in situ and bulk sulfur isotope data are much more complex than δ34S values permitted by prevailing genetic models that invoke only biogenically reduced sulfur and coeval deposition of galena, sphalerite, and framboidal pyrite within a euxinic water column, and we present several lines of evidence that argue

  7. Association of the herpes simplex virus type 1 Us11 gene product with the cellular kinesin light-chain-related protein PAT1 results in the redistribution of both polypeptides.

    PubMed

    Benboudjema, Louisa; Mulvey, Matthew; Gao, Yuehua; Pimplikar, Sanjay W; Mohr, Ian

    2003-09-01

    The herpes simplex virus type 1 (HSV-1) Us11 gene encodes a multifunctional double-stranded RNA (dsRNA)-binding protein that is expressed late in infection and packaged into the tegument layer of the virus particle. As a tegument component, Us11 associates with nascent capsids after its synthesis late in the infectious cycle and is delivered into newly infected cells at times prior to the expression of viral genes. Us11 is also an abundant late protein that regulates translation through its association with host components and contains overlapping nucleolar retention and nuclear export signals, allowing its accumulation in both nucleoli and the cytosol. Thus, at various times during the viral life cycle and in different intracellular compartments, Us11 has the potential to execute discrete tasks. The analysis of these functions, however, is complicated by the fact that Us11 is not essential for viral replication in cultured cells. To discover new host targets for the Us11 protein, we searched for cellular proteins that interact with Us11 and have identified PAT1 as a Us11-binding protein according to multiple, independent experimental criteria. PAT1 binds microtubules, participates in amyloid precursor protein trafficking, and has homology to the kinesin light chain (KLC) in its carboxyl terminus. The carboxyl-terminal dsRNA-binding domain of Us11, which also contains the nucleolar retention and nuclear export signals, binds PAT1, whereas 149 residues derived from the KLC homology region of PAT1 are important for binding to Us11. Both PAT1 and Us11 colocalize within a perinuclear area in transiently transfected and HSV-1-infected cells. The 149 amino acids derived from the KLC homology region are required for colocalization of the two polypeptides. Furthermore, although PAT1 normally accumulates in the nuclear compartment, Us11 expression results in the exclusion of PAT1 from the nucleus and its accumulation in the perinuclear space. Similarly, Us11 does not

  8. The Notting Dale Urban Studies Centre

    ERIC Educational Resources Information Center

    Webb, Chris; Lynas, Sue

    1976-01-01

    Founded in 1974, the Centre is one of the most intensively used resource centres in the United Kingdom. Adults and students from elementary to college level use its facilities to learn about the urban environment. (BD)

  9. Seasonal migration and environmental conditions of Pacific halibut Hippoglossus stenolepis, elucidated from pop-up archival transmitting (PAT) tags

    USGS Publications Warehouse

    Loher, T.; Seitz, A.

    2006-01-01

    Pop-up archival transmitting (PAT) tags were used to study the fall migration of halibut in the Gulf of Alaska (GOA). We tagged 6 Pacific halibut Hippoglossus stenolepis on summer feeding grounds in the eastern GOA and another 6 in the western GOA from June 13 to August 6, 2002. The tags were programed to be released from the fish on January 15, 2003, at the height of the winter spawning season: 10 tags successfully detached, transmitted archived environmental data (depth and temperature), and generated accurate latitude-longitude coordinates shortly after pop-up; 2 tags deployed off SE Alaska were lost. The tags revealed that 6 fish had moved a considerable distance (>200 km) between tagging and pop-up, and all of these had moved northward to some extent. The longest of the observed migrations was from the southern Alaska Peninsula to Yakutat Bay, a linear displacement of 1153 km; 4 fish showed little evidence of geographic displacement, exhibiting migrations that ranged only from 30 to 69 km. Although 2 fish had moved inshore by the end of the tagging period, all other fish had moved offshore regardless of their overall migration distance. The precise timing of offshore movements varied, beginning as early as August and as late as January. These observations generally corroborate conventional tagging, indicating migration of halibut toward winter spawning grounds in the northern GOA, and movement of fish to deep water in fall. However, no single stereotypic migration behavior was apparent, and a variety of vertical movement patterns and temperature profiles were observed. Halibut spent most time in waters of 5 to 7??C, but experienced temperatures ranging from 2.6 to 11.6??C. Depth observations ranged from 0 to 736 m, with summertime activity concentrated in depths from 0 to 400 m, and halibut that exhibited offshore movement were typically observed at 300 to 700 m by mid-winter. Vertical movement (short-period changes in depth) varied among fish and over time

  10. Constitutive expression of McCHIT1-PAT enhances resistance to rice blast and herbicide, but does not affect grain yield in transgenic glutinous rice.

    PubMed

    Zeng, Xiao-Fang; Li, Lei; Li, Jian-Rong; Zhao, De-Gang

    2016-01-01

    To produce new rice blast- and herbicide-resistant transgenic rice lines, the McCHIT1 gene encoding the class I chitinase from Momordica charantia and the herbicide resistance gene PAT were introduced into Lailong (Oryza sativa L. ssp. Japonica), a glutinous local rice variety from Guizhou Province, People's Republic of China. Transgenic lines were identified by ß-glucuronidase (GUS) histochemical staining, PCR, and Southern blot analyses. Agronomic traits, resistance to rice blast and herbicide, chitinase activities, and transcript levels of McCHIT1 were assessed in the T2 progeny of three transgenic lines (L1, L8, and L10). The results showed that the introduction of McCHIT1-PAT into Lailong significantly enhanced herbicide and blast resistance. After infection with the blast fungus Magnaporthe oryzae, all of the T2 progeny exhibited less severe lesion symptoms than those of wild type. The disease indices were 100% for wild type, 65.66% for T2 transgenic line L1, 59.69% for T2 transgenic line L8, and 79.80% for T2 transgenic line L10. Transgenic lines expressing McCHIT1-PAT did not show a significant difference from wild type in terms of malondialdehyde (MDA) content, polyphenol oxidase (PPO) activity, and superoxide dismutase (SOD) activity in the leaves. However, after inoculation with M. oryzae, transgenic plants showed significantly higher SOD and PPO activities and lower MDA contents in leaves, compared with those in wild-type leaves. The transgenic and the wild-type plants did not show significant differences in grain yield parameters including plant height, panicles per plant, seeds per panicle, and 1000-grain weight. Therefore, the transgenic plants showed increased herbicide and blast resistance, with no yield penalty. PMID:25639923

  11. [The Patient Rights Act (PatRG)--part 2: informed consent, duty to inform, documentation of treatment, access to the patient file].

    PubMed

    Parzeller, Markus; Zedler, Barbara

    2013-01-01

    The article deals with the new regulations in the German Civil Code (BGB) which came into effect in Germany on 26 Feb 2013 as the Patient Rights Act (PatRG). In the second part, the consent of the patient (Section 630d Civil Code), the physician's duties to inform the patient (Section 630e Civil Code), the documentation of treatment (Section 630f Civil Code), and the right of access to the patient file (Section 630g Civil Code) are discussed and critically analysed. PMID:24547617

  12. Nuclear microprobe determination of platinum quantitative distribution in rat brain tumors after cisplatin or carboplatin injection for PAT treatment of glioma

    NASA Astrophysics Data System (ADS)

    Ortega, R.; Biston, M.-C.; Devès, G.; Bohic, S.; Carmona, A.

    2005-04-01

    Conventional radiotherapy of high-grade glioma is unsuccessful since less than 50% of patients survive at 6 months, therefore glioma treatment is still challenging. A new radiotherapy procedure has been recently proposed, the photoactivation therapy (PAT), associating synchrotron radiation with a chemotherapy agent, such as cisplatin. PAT aims at using the monochromaticity and the very high brilliance of the synchrotron radiation for selective excitation of a high-Z compound introduced in tumor cell DNA to maximize the photoelectric effect probability, thus increasing local toxicity. Synchrotron irradiation of cisplatin at the platinum absorption K-edge resulted in a dramatic increase in life span relative to median survival time in the F98 glioma model in Fisher rat. In the purpose to optimize the platinum concentration into the tumor, the platinum content of irradiated target needs to be quantified. These results will enable to correlate injected dose to cellular platinum content in the tumor at the time of irradiation, and to study the spatial diffusion and distribution of the platinum into the tumor and the surrounding healthy tissues from the point of injection. Male Fisher 344 rats were inoculated with 103 F98 glioma cells. Thirteen days after stereotactic inoculation, intracerebral injection at the tumor site of 40 μg of carboplatin and 3 or 5 μg of cisplatin was performed. Platinum quantitative distribution in tumors and adjacent brain tissues was determined using μ-PIXE and μ-RBS analysis.

  13. Polyunsaturated fatty acid relatively decreases cholesterol content in THP-1 macrophage-derived foam cell: partly correlates with expression profile of CIDE and PAT members

    PubMed Central

    2013-01-01

    Background Polyunsaturated fatty acids (PUFAs) have positive effect on the regulation of plasma lipids. But the mechanism for them to modulate lipid homeostasis in macrophage is still unclear. In this study, we employed PUFA to pretreat macrophages and evaluated the variations of lipid droplet (LD) content, lipid composition, and expressions of LD-associated genes in macrophage-derived foam cells. Method THP-1-derived macrophages or human peripheral blood monocyte-derived macrophages were pre-treated with four non-esterified fatty acids (NEFAs) separately: saturated fatty acid (SFA)-palmitic acid (PA), monounsaturated fatty acids (MUFAs)-oleic acid (OA), PUFAs-linoleic acid (LA) and eicosapentaenoic acid (EPA). Intracellular lipid content and cholesterol efflux were analyzed in THP-1 macrophage-derived foam cells. Related gene expressions were detected by quantitative real-time PCR. Results PUFA pre-treatment reduced cholesterol content in foam cells and increased cholesterol efflux to lipid-free apoAI in conditioned medium compared with PA or OA group. Cell death-inducing DFF45 like effector (CIDE) and Perilipin-Adipophilin-TIP47 (PAT) family members, as LD-associated proteins, showed specific gene expression profiles after PUFA pre-treatment. These results may help to explain the process of lipid metabolism within foam cells. Conclusion PUFA (LA or EPA) had a potential protective effect against cholesterol accumulation. The specific expressions of CIDE and PAT genes may provide clues to explore the protective mechanism of PUFA in foam cells. PMID:23879935

  14. Implementation of an advanced hybrid MPC-PID control system using PAT tools into a direct compaction continuous pharmaceutical tablet manufacturing pilot plant.

    PubMed

    Singh, Ravendra; Sahay, Abhishek; Karry, Krizia M; Muzzio, Fernando; Ierapetritou, Marianthi; Ramachandran, Rohit

    2014-10-01

    It is desirable for a pharmaceutical final dosage form to be manufactured through a quality by design (QbD)-based approach rather than a quality by testing (QbT) approach. An automatic feedback control system coupled with PAT tools that is part of the QbD paradigm shift, has the potential to ensure that the pre-defined end product quality attributes are met in a time and cost efficient manner. In this work, an advanced hybrid MPC-PID control architecture coupled with real time inline/online monitoring tools and principal components analysis (PCA) based additional supervisory control layer has been proposed for a continuous direct compaction tablet manufacturing process. The advantages of both MPC and PID have been utilized in a hybrid scheme. The control hardware and software integration and implementation of the control system has been demonstrated using feeders and blending unit operation of a continuous tablet manufacturing pilot plant and an NIR based PAT tool. The advanced hybrid MPC-PID control scheme leads to enhanced control loop performance of the critical quality attributes in comparison to a regulatory (e.g. PID) control scheme indicating its potential to improve pharmaceutical product quality. PMID:24974987

  15. Community-based outpatient parenteral antimicrobial therapy (CoPAT) for Staphylococcus aureus bacteraemia with or without infective endocarditis: analysis of the randomized trial comparing daptomycin with standard therapy

    PubMed Central

    Rehm, Susan; Campion, Marilyn; Katz, David E.; Russo, Rene; Boucher, Helen W.

    2009-01-01

    Objectives Administering outpatient parenteral antimicrobial therapy in the community setting (CoPAT) is becoming more common with the increasing emphasis on controlling costs. However, few controlled trials have evaluated this treatment modality. Methods Using data from a recent randomized trial comparing daptomycin with standard therapy (semi-synthetic penicillin or vancomycin, each with initial low-dose gentamicin) for Staphylococcus aureus bacteraemia and infective endocarditis (SAB/IE), patient characteristics and outcomes were evaluated. Patients receiving their full course of therapy in the hospital setting were compared with those who received some portion outside of the hospital (CoPAT). Results Among the 200 patients, 51.5% received CoPAT. These patients were generally younger (median age 50 versus 54 years, P = 0.028). In the CoPAT group, there tended to be fewer patients with endocardial involvement (8.7% versus 18.6%, P = 0.061) and pre-existing valvular heart disease (7.8% versus 15.5%, P = 0.120). CoPAT patients received longer therapy courses (mean 25.4 versus 13.5 days, P < 0.001) and had higher rates of therapy completion (90.3% versus 45.4%, P < 0.001) and clinical success (86.4% versus 55.7%, P < 0.001). Persisting or relapsing S. aureus was less frequent in the CoPAT group (3.9% versus 15.5%, P = 0.007) and there were fewer deaths (3.9% versus 18.6%, P = 0.001) 6 weeks after the end of therapy. Hospital readmission occurred for 18 of the 103 (17.5%) CoPAT patients. Clinical success rates were similar for CoPAT patients receiving daptomycin (90.0%) or standard therapy (83.0%). Conclusions With proper monitoring, stable patients can complete treatment for SAB/IE as outpatients in the community setting. Daptomycin is an appropriate option for this setting. PMID:19264792

  16. Green synthesis and antimicrobial activity of silver nanoparticles using wild medicinal mushroom Ganoderma applanatum (Pers.) Pat. from Similipal Biosphere Reserve, Odisha, India.

    PubMed

    Mohanta, Yugal Kishore; Singdevsachan, Sameer Kumar; Parida, Umesh Kumar; Panda, Sujogya Kumar; Mohanta, Tapan Kumar; Bae, Hanhong

    2016-08-01

    In the present study, green synthesis and cost effective approach of silver nanoparticles using wild medicinal mushroom Ganoderma applanatum (Pers.) Pat. from Similipal Biosphere Reserve, Odisha, India is reported. The biosynthesised AgNPs were characterised using UV-visible spectroscopy, particle analyser and scanning electron microscopy studies. It was found by dynamic light scattering analysis, that the average size and charges of the AgNPs were 133.0 ± 0.361 nm and -6.01 ± 5.30 mV, respectively. Moreover, the Fourier transform infrared study was also conducted to identify the biomolecules or functional groups responsible for the reduction of Ag and stabilisation of the AgNPs. The potential biomedical application with reference to antimicrobial activity of the synthesised AgNPs was investigated against some pathogenic microorganisms viz. Escherichia coli, Bacillus subtilis, Staphylococcus epidermidis, Vibrio cholerae, Staphylococcus aureus and Shigella flexneri. PMID:27463787

  17. Recent advances in mass spectrometry analysis of histone post-translational modifications: potential clinical impact of the PAT-H-MS approach.

    PubMed

    Bonaldi, Tiziana; Noberini, Roberta

    2016-03-01

    Histone post-translational modifications (hPTMs) contribute to the regulation of gene expression and increasing evidence links them to the development of various pathologies, highlighting their potential as biomarkers for prognostic, diagnostic and therapeutic applications. Mass spectrometry (MS) has emerged as a powerful analytical tool for hPTM analysis, which has also been applied to the analysis of epigenetic aberrations in diseases. However, the potential offered by the MS-based hPTM analysis of clinical samples for epigenetic biomarker discovery has been left largely unexploited. This article summarizes the contribution of MS-based approaches to clinical epigenetics, with a special focus on the PAThology tissue analysis of Histones by Mass Spectrometry (PAT-H-MS) approach - which represents the first application of MS-based hPTM analysis to formalin-fixed paraffin-embedded clinical samples - discussing its strengths and limitations, as well as possible implementations. PMID:26822344

  18. Kagami-Ogata syndrome: a clinically recognizable upd(14)pat and related disorder affecting the chromosome 14q32.2 imprinted region.

    PubMed

    Ogata, Tsutomu; Kagami, Masayo

    2016-02-01

    Human chromosome 14q32.2 carries paternally expressed genes including DLK1 and RTL1, and maternally expressed genes including MEG3 and RTL1as, along with the germline-derived DLK1-MEG3 intergenic differentially methylated region (IG-DMR) and the postfertilization-derived MEG3-DMR. Consistent with this, paternal uniparental disomy 14 (upd(14)pat), and epimutations (hypermethylations) and microdeletions affecting the IG-DMR and/or the MEG3-DMR of maternal origin, result in a unique phenotype associated with characteristic face, a small bell-shaped thorax with coat-hanger appearance of the ribs, abdominal wall defects, placentomegaly and polyhydramnios. Recently, the name 'Kagami-Ogata syndrome' (KOS) has been approved for this clinically recognizable disorder. Here, we review the current knowledge about KOS. Important findings include the following: (1) the facial 'gestalt' and the increased coat-hanger angle constitute pathognomonic features from infancy through childhood/puberty; (2) the unmethylated IG-DMR and MEG3-DMR of maternal origin function as the imprinting control centers in the placenta and body respectively, with a hierarchical interaction regulated by the IG-DMR for the methylation pattern of the MEG3-DMR in the body; (3) RTL1 expression level becomes ~2.5 times increased in the absence of functional RTL1as-encoded microRNAs that act as a trans-acting repressor for RTL1; (4) excessive RTL1 expression and absent MEG expression constitute the primary underlying factor for the phenotypic development; and (5) upd(14)pat accounts for approximately two-thirds of KOS patients, and epimutations and microdeletions are identified with a similar frequency. Furthermore, we refer to diagnostic and therapeutic implications. PMID:26377239

  19. Kagami–Ogata syndrome: a clinically recognizable upd(14)pat and related disorder affecting the chromosome 14q32.2 imprinted region

    PubMed Central

    Ogata, Tsutomu; Kagami, Masayo

    2016-01-01

    Human chromosome 14q32.2 carries paternally expressed genes including DLK1 and RTL1, and maternally expressed genes including MEG3 and RTL1as, along with the germline-derived DLK1-MEG3 intergenic differentially methylated region (IG-DMR) and the postfertilization-derived MEG3-DMR. Consistent with this, paternal uniparental disomy 14 (upd(14)pat), and epimutations (hypermethylations) and microdeletions affecting the IG-DMR and/or the MEG3-DMR of maternal origin, result in a unique phenotype associated with characteristic face, a small bell-shaped thorax with coat-hanger appearance of the ribs, abdominal wall defects, placentomegaly and polyhydramnios. Recently, the name ‘Kagami–Ogata syndrome' (KOS) has been approved for this clinically recognizable disorder. Here, we review the current knowledge about KOS. Important findings include the following: (1) the facial ‘gestalt' and the increased coat-hanger angle constitute pathognomonic features from infancy through childhood/puberty; (2) the unmethylated IG-DMR and MEG3-DMR of maternal origin function as the imprinting control centers in the placenta and body respectively, with a hierarchical interaction regulated by the IG-DMR for the methylation pattern of the MEG3-DMR in the body; (3) RTL1 expression level becomes ~2.5 times increased in the absence of functional RTL1as-encoded microRNAs that act as a trans-acting repressor for RTL1; (4) excessive RTL1 expression and absent MEG expression constitute the primary underlying factor for the phenotypic development; and (5) upd(14)pat accounts for approximately two-thirds of KOS patients, and epimutations and microdeletions are identified with a similar frequency. Furthermore, we refer to diagnostic and therapeutic implications. PMID:26377239

  20. An integrated process analytical technology (PAT) approach to monitoring the effect of supercooling on lyophilization product and process parameters of model monoclonal antibody formulations.

    PubMed

    Awotwe-Otoo, David; Agarabi, Cyrus; Khan, Mansoor A

    2014-07-01

    The aim of the present study was to apply an integrated process analytical technology (PAT) approach to control and monitor the effect of the degree of supercooling on critical process and product parameters of a lyophilization cycle. Two concentrations of a mAb formulation were used as models for lyophilization. ControLyo™ technology was applied to control the onset of ice nucleation, whereas tunable diode laser absorption spectroscopy (TDLAS) was utilized as a noninvasive tool for the inline monitoring of the water vapor concentration and vapor flow velocity in the spool during primary drying. The instantaneous measurements were then used to determine the effect of the degree of supercooling on critical process and product parameters. Controlled nucleation resulted in uniform nucleation at lower degrees of supercooling for both formulations, higher sublimation rates, lower mass transfer resistance, lower product temperatures at the sublimation interface, and shorter primary drying times compared with the conventional shelf-ramped freezing. Controlled nucleation also resulted in lyophilized cakes with more elegant and porous structure with no visible collapse or shrinkage, lower specific surface area, and shorter reconstitution times compared with the uncontrolled nucleation. Uncontrolled nucleation however resulted in lyophilized cakes with relatively lower residual moisture contents compared with controlled nucleation. TDLAS proved to be an efficient tool to determine the endpoint of primary drying. There was good agreement between data obtained from TDLAS-based measurements and SMART™ technology. ControLyo™ technology and TDLAS showed great potential as PAT tools to achieve enhanced process monitoring and control during lyophilization cycles. PMID:24840395

  1. Polymorphic amplified typing sequences (PATS) and pulsed-field gel electrophoresis (PFGE) yield comparable results in the strain typing of a diverse set of bovine Escherichia coli O157 isolates

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The PCR-based Escherichia coli O157 (O157) strain typing system, Polymorphic Amplified Typing Sequences (PATS), targets insertions-deletions (Indels) and single nucleotide polymorphisms (SNPs) at the XbaI and AvrII(BlnI) restriction enzyme sites, respectively, besides amplifying four known virulenc...

  2. CaMKII-MEDIATED PHOSPHORYLATION OF THE BOMBYX MORI LIPID STORAGE DROPLET PROTEIN-1 (BmLsd1), AN INSECT PAT FAMILY PROTEIN, IS ESSENTIAL FOR SILKMOTH SEX PHEROMONE BIOSYNTHESIS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The structurally-related members of the PAT family of proteins, which are so name based on similarity amongst perilipin, adipophilin/adipocyte differentiation-related protein (ADRP), and tail-interacting protein of 47 kilodaltons (TIP47), are cytoplasmic lipid droplet (LD)-associated proteins charac...

  3. Hormone signaling linked to silkmoth sex pheromone biosynthesis involves Ca2+/calmodulin-dependent protein kinase II-mediated phosphorylation of the insect PAT family protein Bombyx mori lipid storage droplet protein-1(BmLsd)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The structurally-related members of the PAT family of proteins, which are so name based on similarity amongst perilipin, adipophilin/adipocyte differentiation-related protein (ADRP), and tail-interacting protein of 47 kilodaltons (TIP47), are cytoplasmic lipid droplet (LD)-associated proteins charac...

  4. Selective irreversible blockade of 5-hydroxytryptamine1A and 5-hydroxytryptamine1C receptor binding sites in the rat brain by 8-MeO-2'-chloro-PAT: a quantitative autoradiographic study.

    PubMed

    Radja, F; Daval, G; Emerit, M B; Gallissot, M C; Hamon, M; Vergé, D

    1989-01-01

    The possible irreversible blockade of 5-hydroxytryptamine1 receptor subtypes 5-hydroxytryptamine1A, 5-hydroxytryptamine1B/5-hydroxytryptamine1D and 5-hydroxytryptamine1C by the chloramine 8-methoxy-2-(N-2'-chloropropyl,N-propyl)aminotetralin (8-MeO-2'-chloro-PAT) was investigated in rat brain sections by quantitative autoradiography using [3H]8-hydroxy-2-(di-n-propylamino)tetralin [( 3H]8-OH-DPAT), [3H]5-hydroxytryptamine, [125I]BH-8-MeO-N-PAT and [125I]cyanopindolol as radio-ligands. A marked reduction (-50% to -75%) of [3H]8-OH-DPAT and [125I]BH-8-MeO-N-PAT specific binding to 5-hydroxytryptamine1A sites in the hippocampus (CA1 area) and the dorsal raphe nucleus, and of [3H]5-hydroxytryptamine specific binding to 5-hydroxytryptamine1C sites in the choroid plexus was found in sections exposed to 1 microM 8-MeO-2'-chloro-PAT and then washed extensively. In contrast the specific binding of [3H]5-hydroxytryptamine to 5-hydroxytryptamine1B/5-hydroxytryptamine1D sites and of [125I]cyanopindolol to 5-hydroxytryptamine1B sites in the substantia nigra and dorsal subiculum remained unaltered by this treatment. Similarly [125I]cyanopindolol binding to beta-adrenergic receptors was not affected by 8-MeO-2'-chloro-PAT. Prior occupancy of 5-hydroxytryptamine1A sites by 10 microM 5-hydroxytryptamine or 8-OH-DPAT, and of 5-hydroxytryptamine1C sites by 10 microM 5-hydroxytryptamine prevented any subsequent blockade by 8-MeO-2'-chloro-PAT. These data indicate that 8-MeO-2'-chloro-PAT should be a useful alkylating agent for achieving selective irreversible blockade of central 5-hydroxytryptamine1A and 5-hydroxytryptamine1C receptors in vivo in the rat. PMID:2531850

  5. Taurine uptake across the human intestinal brush-border membrane is via two transporters: H+-coupled PAT1 (SLC36A1) and Na+- and Cl−-dependent TauT (SLC6A6)

    PubMed Central

    Anderson, Catriona M H; Howard, Alison; Walters, Julian R F; Ganapathy, Vadivel; Thwaites, David T

    2009-01-01

    Taurine is an essential amino acid in some mammals and is conditionally essential in humans. Taurine is an abundant component of meat and fish-based foods and has been used as an oral supplement in the treatment of disorders such as cystic fibrosis and hypertension. The purpose of this investigation was to identity the relative contributions of the solute transporters involved in taurine uptake across the luminal membrane of human enterocytes. Distinct transport characteristics were revealed following expression of the candidate solute transporters in Xenopus laevis oocytes: PAT1 (SLC36A1) is a H+-coupled, pH-dependent, Na+- and Cl−-independent, low-affinity, high-capacity transporter for taurine and β-alanine; TauT (SLC6A6) is a Na+- and Cl−-dependent, high-affinity, low-capacity transporter of taurine and β-alanine; ATB0,+ (SLC6A14) is a Na+- and Cl−-dependent, high-affinity, low-capacity transporter which accepts β-alanine but not taurine. Taurine uptake across the brush-border membrane of human intestinal Caco-2 cell monolayers showed characteristics of both PAT1- and TauT-mediated transport. Under physiological conditions, Cl−-dependent TauT-mediated uptake predominates at low taurine concentrations, whereas at higher concentrations typical of diet, Cl−-independent PAT1-mediated uptake is the major absorptive mechanism. Real-time PCR analysis of human duodenal and ileal biopsy samples demonstrates that PAT1, TauT and ATB0,+ mRNA are expressed in each tissue but to varying degrees. In conclusion, this study is the first to demonstrate both taurine uptake via PAT1 and functional coexpression of PAT1 and TauT at the apical membrane of the human intestinal epithelium. PAT1 may be responsible for bulk taurine uptake during a meal whereas TauT may be important for taurine supply to the intestinal epithelium and for taurine capture between meals. PMID:19074966

  6. (1R, 3S)-(−)-Trans-PAT: A novel full-efficacy serotonin 5-HT2C receptor agonist with 5-HT2A and 5-HT2B receptor inverse agonist/antagonist activity

    PubMed Central

    Booth, Raymond G.; Fang, Lijuan; Huang, Yingsu; Wilczynski, Andrzej; Sivendran, Sashikala

    2009-01-01

    The serotonin 5-HT2A, 5-HT2B, and 5-HT2C G protein-coupled receptors signal primarily through Gαq to activate phospholipase C (PLC) and formation of inositol phosphates (IP) and diacylglycerol. The human 5-HT2C receptor, expressed exclusively in the central nervous system, is involved in several physiological and psychological processes. Development of 5-HT2C agonists that do not also activate 5-HT2A or 5-HT2B receptors is challenging because transmembrane domain identity is about 75% among 5-HT2 subtypes. This paper reports 5-HT2 receptor affinity and function of (1R,3S)-(−)-trans-1-phenyl-3-dimethylamino-1,2,3,4-tetrahydronaphthalene (PAT), a small molecule that produces anorexia and weight-loss after peripheral administration to mice. (−)-Trans-PAT is a stereoselective full-efficacy agonist at human 5-HT2C receptors, plus, it is a 5-HT2A/5-HT2B inverse agonist and competitive antagonist. The Ki of (−)-trans-PAT at 5-HT2A, 5-HT2B, and 5-HT2C receptors is 410, 1200, and 37 nM, respectively. Functional studies measured activation of PLC/[3H]-IP formation in clonal cells expressing human 5-HT2 receptors. At 5-HT2C receptors, (−)-trans-PAT is an agonist (EC50 = 20 nM) comparable to serotonin in potency and efficacy. At 5-HT2A and 5-HT2B receptors, (−)-trans-PAT is an inverse agonist (IC50 = 490 and 1,000 nM, respectively) and competitive antagonist (KB = 460 and 1400 nM, respectively) of serotonin. Experimental results are interpreted in light of molecular modeling studies indicating the (−)-trans-PAT protonated amine can form an ionic bond with D3.32 of 5-HT2A and 5-HT2C receptors, but, not with 5-HT2B receptors. In addition to probing 5-HT2 receptor structure and function, (−)-trans-PAT is a novel lead regarding 5-HT2C agonist/5-HT2A inverse agonist drug development for obesity and neuropsychiatric disorders. PMID:19397907

  7. Implementation of an artificial neural network as a PAT tool for the prediction of temperature distribution within a pharmaceutical fluidized bed granulator.

    PubMed

    Korteby, Yasmine; Mahdi, Yassine; Azizou, Amel; Daoud, Kamel; Regdon, Géza

    2016-06-10

    In this study, a novel in-line measurement technique of the air temperature distribution during a granulation process using a conical fluidized bed was designed and built for the purpose of measuring the temperature under the Process Analytical Technology (PAT) and introduced to predict the establishment of temperature profiles. Three sets of thermocouples were used, placed at different positions covering the whole operating range, connected to data acquisition measurement hardware, allowing an in-line acquisition and recording of temperatures every second. The measurements throughout the fluidized bed were performed in a steady state by spraying a solution of PVP onto a lactose monohydrate powder bed in order to make predictions of the temperature distribution and the hydrodynamics of the bed during the granulation process using Artificial Neural Networks (ANNs) and to establish the different temperature profiles for different process conditions through the precise predicted information by the constructed, trained, validated and tested neural network. The model's testing results showed a strong prediction capacity of the effects of process variables. Indeed, the predicted temperature values obtained with the ANN model were in good agreement with the values measured with in-line reference method and hence the method can have an application as a predictive control tool. PMID:26993961

  8. Obstructive Sleep Apnea Using Watch-PAT 200 Is Independently Associated With an Increase in Morning Blood Pressure Surge in Never-Treated Hypertensive Patients.

    PubMed

    Cho, Jung Sun; Ihm, Sang-Hyun; Kim, Chan Joon; Park, Mahn-Won; Her, Sung-Ho; Park, Gyung-Min; Kim, Tae-Seok

    2015-09-01

    This study aimed to examine the association between obstructive sleep apnea (OSA) and morning blood pressure surge in never-treated patients with essential hypertension. This prospective study included a total of 58 patients (mean age, 51.7 years; 55.2% men) with never-treated essential hypertension. The patients were divided into non-OSA (n=23, 49.3±12.7 years) and OSA (n=35, 53.2±9.8 years) groups. The OSA group was defined as having an apnea-hypopnea index level >5 as measured by the Watch-PAT 200. The authors collected 24-hour ambulatory BP, plasma aldosterone concentration, and plasma renin activity data from all of the patients. The measured sleep-trough morning systolic blood pressure (SBP) increases were higher in the OSA group than in the non-OSA group (28.7±11.8 mm Hg vs 19.6±12.8 mm Hg, P=.008). The sleep-trough morning SBP increase was inversely correlated with the lowest oxygen saturation (r=-0.272, P=.039). OSA known to be associated with increased daytime and nocturnal sympathetic activity was associated with significantly higher sleep-trough morning SBP levels in this study. PMID:26033308

  9. Compositional equivalence of DAS-444Ø6-6 (AAD-12 + 2mEPSPS + PAT) herbicide-tolerant soybean and nontransgenic soybean.

    PubMed

    Lepping, Miles D; Herman, Rod A; Potts, Brian L

    2013-11-20

    Soybeans from transgenic event DAS-444Ø6-6 are the first to express three proteins that provide tolerance to broad-spectrum herbicides. DAS-444Ø6-6 soybean expresses the aryloxyalkanoate dioxygenase-12 (AAD-12) enzyme from the soil bacterium Delftia acidovorans , which provides tolerance to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D); the double-mutant 5-enolpyruvylshikimate-3-phosphate synthase (2mEPSPS) enzyme encoded by a modified version of the epsps gene from maize ( Zea mays ), which provides tolerance to the herbicide glyphosate; and the phosphinothricin acetyltransferase (PAT) enzyme from Streptomyces viridochromogenes , which provides tolerance to the herbicide glufosinate. The purpose of this study was to determine if the nutrient and antinutrient composition of forage and grain from DAS-444Ø6-6 soybean are similar to those of nontransgenic soybean. Forage was analyzed for proximates, fiber, and minerals; grain analyses further included vitamins, amino acid and fatty acid profiles, and antinutrients and bioactive components (lectin, phytic acid, raffinose, stachyose, trypsin inhibitor, and isoflavones). Results indicate that DAS-444Ø6-6 soybean is compositionally equivalent to nontransgenic soybean. Findings are consistent with similar studies for other input traits, as endogenous plant metabolic pathways that influence composition are expected to be less affected by transgenesis compared with traditional plant-breeding methods. PMID:24191699

  10. Development of a Process Analytical Technology (PAT) for in-line monitoring of film thickness and mass of coating materials during a pan coating operation.

    PubMed

    Gendre, Claire; Genty, Muriel; Boiret, Mathieu; Julien, Marc; Meunier, Loïc; Lecoq, Olivier; Baron, Michel; Chaminade, Pierre; Péan, Jean Manuel

    2011-07-17

    The aim of this study was to perform in-line Near Infrared (NIR) measurements inside a pan coater to monitor a coating operation in real-time, by predicting the increases in mass of coating materials and coating thickness. A polymer combination of ethylcellulose/poly(vinyl-alcohol)-poly(ethylene-glycol) graft copolymer was used as functional aqueous coating. Coated tablets were sampled at regular intervals during the coating operation, then subjected to either simple and fast weighing (n=50) or accurate and non-destructive Terahertz Pulsed Imaging (TPI) measurements (n=3). Off-line NIR spectra analysis revealed that the coating operation could efficiently be controlled by focusing on two distinct NIR regions, related to absorption bands of ethylcellulose. Principal component analysis of in-line NIR spectra gave a clear classification of the collected coated tablets. Real-time quantitative monitoring of the coating operation was successfully performed from partial least square calibration models built using either TPI or weighing as reference method. Coating thicknesses as well as mass of coating materials used as primary values provided accurate NIR predictions. A comparison study demonstrated that both reference methods led to reliable and accurate real-time monitoring of the coating operation. This work demonstrated that in-line NIR measurements associated with multivariate analyses can be implemented to monitor in real-time a pan coating operation in order to fulfil the expectations of ICH Q8 guideline on pharmaceutical development, especially in terms of PAT control strategy and reduced end-product testing. PMID:21569842

  11. The world-class Howard's Pass SEDEX Zn-Pb district, Selwyn Basin, Yukon. Part I: trace element compositions of pyrite record input of hydrothermal, diagenetic, and metamorphic fluids to mineralization

    NASA Astrophysics Data System (ADS)

    Gadd, Michael G.; Layton-Matthews, Daniel; Peter, Jan M.; Paradis, Suzanne J.

    2016-03-01

    The Howard's Pass district, located in Yukon Territory, comprises 14 Zn-Pb sedimentary exhalative (SEDEX) deposits that collectively contain approximately 400.7 Mt grading at 4.5 % Zn and 1.5 % Pb. Sulfide mineralization is hosted in carbonaceous and calcareous to siliceous mudstones. Pyrite is a minor but ubiquitous component. Detailed petrographic analyses reveal that pyrite has a complex and protracted growth history, and multiple generations of pyrite are preserved in single grains. Combined electron probe microanalysis (EPMA) and laser ablation-inductively coupled mass plasma-mass spectrometry (LA-ICP-MS) of paragenetically complex pyrite reveal minor and trace element zonation that mimic textural features. These data provide information on the relative timing and cation content of depositional (i.e., ambient marine), hydrothermal, and metamorphic fluids. These data also identify a suite of nonore elements (Mn, As, Ag, Sb, and Tl) associated with the Zn-Pb mineralizing hydrothermal fluids. Lithogeochemical data and statistical results corroborate the microanalytical findings. These elements are associated with both syngenetic to earliest diagenetic pyrite and later diagenetic pyrite overgrowths, suggesting that SEDEX mineralization was not only the product of hydrothermal precipitates that settled on the seafloor, but also dense metalliferous brine also settled on, and percolated through, unconsolidated carbonaceous muds and precipitated metals. This genetic model is similar to that proposed for the Paleoproterozoic HYC Zn-Pb-Ag SEDEX deposit in northern Australia, and it is likely that common processes and ambient conditions led to the formation and preservation of both of these large SEDEX districts.

  12. Howard Parnes, MD | Division of Cancer Prevention

    Cancer.gov

    The Division of Cancer Prevention (DCP) conducts and supports research to determine a person's risk of cancer and to find ways to reduce the risk. This knowledge is critical to making progress against cancer because risk varies over the lifespan as genetic and epigenetic changes can transform healthy tissue into invasive cancer.

  13. Historicism, "Huckleberry Finn," and Howard Beach.

    ERIC Educational Resources Information Center

    Puttock, Kay

    1990-01-01

    Argues for the effectiveness of Mark Twain's "Huckleberry Finn" as a tool for increasing racial understanding. Suggests that class discussion of the book can make Black students feel as though they have valuable insights to contribute. Concludes that students' emotional reactions to Twain's novel demonstrate the continuing relevance of the text.…

  14. A DNA electrochemical sensor prepared by electrodepositing zirconia on composite films of single-walled carbon nanotubes and poly(2,6-pyridinedicarboxylic acid), and its application to detection of the PAT gene fragment.

    PubMed

    Yang, Jie; Jiao, Kui; Yang, Tao

    2007-10-01

    Carboxyl group-functionalized single-walled carbon nanotubes (SWNTs) and 2,6-pyridinedicarboxylic acid (PDC) were electropolymerized by cyclic voltammetry on a glassy-carbon electrode (GCE) surface to form composite films (SWNTs/PDC). Zirconia was then electrodeposited on the SWNTs/PDC/GCE from an aqueous electrolyte containing ZrOCl2 and KCl by cycling the potential between -1.1 V and +0.7 V at a scan rate of 20 mV s(-1). DNA probes with a phosphate group at the 5' end were easily immobilized on the zirconia thin films, because of the strong affinity between zirconia and phosphate groups. The sensors were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). EIS was used for label-free detection of the target DNA by measuring the increase of the electron transfer resistance (R(et)) of the electrode surface after the hybridization of the probe DNA with the target DNA. The PAT gene fragment and polymerase chain reaction (PCR) amplification of the NOS gene from transgenically modified beans were satisfactorily detected by use of this DNA electrochemical sensor. The dynamic range of detection of the sensor for the PAT gene fragment was from 1.0 x 10(-11) to 1.0 x 10(-6) mol L(-1) and the detection limit was 1.38 x 10(-12) mol L(-1). PMID:17851654

  15. Advancing Humanities Studies at Community, Technical, and Junior Colleges.

    ERIC Educational Resources Information Center

    Eisenberg, Diane U.; And Others

    The American Association of Community and Junior Colleges' (AACJC's) two-year Advancing the Humanities Project (AHP) has assisted selected community colleges in promoting the humanities on their campuses. Parts I and II of this report on the AHP present statements by Dale Parnell and Judith Jeffrey Howard about the AACJC's humanities initiatives…

  16. Improving Adult Education for the 21st Century. Hearing Before the Subcommittee on 21st Century Competitiveness of the Committee on Education and the Workforce, House of Representatives, One Hundred Eighth Congress, First Session. Hearing held in Washington, DC, March 4, 2003.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and the Workforce.

    A hearing was held to discuss the prior four years of implementation of the Adult Education and Family Literacy Act, and to recommend further improvements. The opening statements of Chairman Howard McKeon and Dale E. Kildee introduce the meeting and discuss the importance of promoting an educated populace that will maintain the United States'…

  17. The Federal Role in K-12 Mathematics Reform. Hearing before the Subcommittee on Early Childhood, Youth and Families of the Committee on Education and the Workforce, House of Representatives, One Hundred Sixth Congress, Second Session (February 2, 2000).

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and the Workforce.

    This hearing focuses on the federal role in K-12 mathematics reform. Prepared statements are provided from: Michael N. Castle, Chairman, Subcommittee on Early Childhood Youth, and Families; Dale E. Kildee, Ranking Member, Subcommittee on Early Childhood Youth, and Families; Howard P. "Buck" Mckeon, Chairman, Subcommittee on Postsecondary…

  18. Book Review: Caves and Karst of the Yorkshire Dales

    NASA Astrophysics Data System (ADS)

    Westaway, Rob

    2015-10-01

    The British Cave Research Association (BCRA) is the research division of the British Caving Association (BCA), itself the principal society in Britain for those interested in caving, with activities including provision of training and safety certification for cavers and managing access to caves. Although some UK cave-related research is carried out by academics, this tends to be restricted to archaeological investigations of caves that have served as human habitations, and to be focused more on the occupants than the caves themselves. In contrast, most cave exploration is undertaken as a leisure activity, under the auspices of clubs affiliated to the BCA/BCRA, this being indeed virtually the only field of Earth science where amateur investigators can continue to make significant discoveries. Many cave explorers are also affiliated with academic researchers, such as managers of dating laboratories; the synergy between these two groups is highly productive, having resulted for instance in the discovery and exploration in recent years of the vast Ogof Draenen cave system in South Wales, which probably dates back to the Early Pleistocene (e.g., Farrant et al., 2014).

  19. Drama in the Dale: Transformation through Community Drama

    ERIC Educational Resources Information Center

    Ward, Julie; Mills, Helen Frances; Anderson, Alan

    2013-01-01

    During the winter of 2011-2012, Weardale, England, was the setting for an ambitious informal adult education project. In this rural area in the northeast part of the country, the local arts collective, Jack Drum Arts, established a community play project entitled "The Bonny Moorhen." This dramatic undertaking aimed to retell the story of…

  20. Dale Long Emergency Medical Service Providers Protection Act

    THOMAS, 112th Congress

    Sen. Leahy, Patrick J. [D-VT

    2011-02-17

    02/17/2011 Read twice and referred to the Committee on the Judiciary. (text of measure as introduced: CR S885) (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  1. "The Day All of the Different Parts of Me Can Come Along": Intersectionality and U.S. Third World Feminism in the Poetry of Pat Parker and Willyce Kim.

    PubMed

    Van Ausdall, Mimi Iimuro

    2015-01-01

    This article brings to light the poetry of Pat Parker and Willyce Kim, two key figures within the 1970s and '80s women in print movement. While Parker and Kim have been rightly placed within African-American and Asian-American histories, respectively, and working-class and lesbian-feminist literary histories, their work is most fully understood within the context of U.S. Third World Feminism. Through close readings of poetic form and content in addition to engagement with current debates about intersectionality as a methodology, the article links Kim and Parker's works to central contributions of U.S. Third World Feminism such as intersectionality and power across and within difference that continue to influence feminist theory today. PMID:26075687

  2. Similar frequency of paternal uniparental disomy involving chromosome 20q (patUPD20q) in Japanese and Caucasian patients affected by sporadic pseudohypoparathyroidism type Ib (sporPHP1B).

    PubMed

    Takatani, Rieko; Minagawa, Masanori; Molinaro, Angelo; Reyes, Monica; Kinoshita, Kaori; Takatani, Tomozumi; Kazukawa, Itsuro; Nagatsuma, Misako; Kashimada, Kenichi; Sato, Kenichi; Matsushita, Kazuyuki; Nomura, Fumio; Shimojo, Naoki; Jüppner, Harald

    2015-10-01

    Pseudohypoparathyroidism type Ib (PHP1B) is caused by proximal tubular resistance to parathyroid hormone that occurs in most cases in the absence of Albright's Hereditary Osteodystrophy (AHO). Familial forms of PHP1B are caused by maternally inherited microdeletions within STX16, the gene encoding syntaxin 16, or within GNAS, a complex genetic locus on chromosome 20q13.3 encoding Gsα and several splice variants thereof. These deletions lead either to a loss-of-methylation affecting GNAS exon A/B alone or to epigenetic changes involving multiple differentially methylated regions (DMRs) within GNAS. Broad GNAS methylation abnormalities are also observed in most sporadic PHP1B (sporPHP1B) cases. However, with the exception of paternal uniparental disomy involving chromosome 20q (patUPD20q), the molecular mechanism leading to this disease variant remains unknown. We now investigated 23 Japanese sporPHP1B cases, who presented with hypocalcemia, hyperphosphatemia, elevated PTH levels, and occasionally with TSH elevations and mild AHO features. Age at diagnosis was 10.6 ± 1.45 years. Calcium, phosphate, and PTH were 6.3 ± 0.23 mg/dL, 7.7 ± 0.33 mg/dL, and 305 ± 34.5 pg/mL, respectively, i.e. laboratory findings that are indistinguishable from those previously observed for Caucasian sporPHP1B cases. All investigated patients showed broad GNAS methylation changes. Eleven individuals were homozygous for SNPs within exon NESP and a pentanucleotide repeat in exon A/B. Two of these patients furthermore revealed homozygosity for numerous microsatellite markers on chromosome 20q raising the possibility of patUPD20q, which was confirmed through the analysis of parental DNA. Based on this and our previous reports, paternal duplication of the chromosomal region comprising the GNAS locus appears to be a fairly common cause of sporPHP1B that is likely to occur with equal frequency in Caucasians and Asians. PMID:25997889

  3. Field Turf System Has Irrigation Down PAT

    ERIC Educational Resources Information Center

    Day, C. William

    1973-01-01

    Explains the process whereby Goshen High School (Indiana) acquired a football field that is never muddy, but which is never covered with expensive sheeting; and that has green grass the year around, but which no one ever sprinkles. It also offers firmness for running, resiliency for falling, traction for turning, and a flat, highly uniform field.…

  4. Howard Coble Coast Guard and Maritime Transportation Act of 2014

    THOMAS, 113th Congress

    Rep. Hunter, Duncan D. [R-CA-50

    2014-12-01

    12/04/2014 Received in the Senate and Read twice and referred to the Committee on Commerce, Science, and Transportation. (All Actions) Notes: For further action, see S.2444, which became Public Law 113-281 on 12/18/2014. Tracker: This bill has the status Passed HouseHere are the steps for Status of Legislation:

  5. The Howard Era--In Retrospect? Border Control

    ERIC Educational Resources Information Center

    Brett, Judith

    2004-01-01

    The last eight years have created radical realignments in Australia's political landscape. The Prime Minister's bitterest enemies are precisely the same people who would once have been Australian Liberalism's stalwarts. The author writes on the legacy of Australia's culture wars. For responses, see EJ848173: "Aussie Battler, or Worldly…

  6. The ethical mind. A conversation with psychologist Howard Gardner.

    PubMed

    Gardner, Howard

    2007-03-01

    Business leadership has become synonymous in the public eye with unethical behavior. Widespread scandals, massive layoffs, and inflated executive pay packages have led many to believe that corporate wrongdoing is the status quo. That's why it's more important than ever that those at the top mend relationships with customers, employees, and other stakeholders. Professor Gardner has spent many years studying the relationship between psychology and ethics at Harvard's Graduate School of Education. In this interview with HBR senior editor Bronwyn Fryer, Gardner talks about what he calls the ethical mind, which helps individuals aspire to do good work that matters to their colleagues, companies, and society in general. In an era when workers are overwhelmed by too much information and feel pressured to win at all costs, Gardner believes, it's easy to lose one's way. What's more, employees look to leaders for cues as to what's appropriate and what's not. So if you're a leader, what's the best way to stand up to ethical pressures and set a good example? First and foremost, says Gardner, you must believe that retaining an ethical compass is essential to the health of your organization. Then you must state your ethical beliefs and stick to them. You should also test yourself rigorously to make sure you're adhering to your values, take time to reflect on your beliefs, find multiple mentors who aren't afraid to speak truth to your power, and confront others' egregious behavior as soon as it arises. In the end, Gardner believes, the world hangs in the balance between right and wrong, good and bad, success and disaster. "You need to decide which side you're on:" he concludes, "and do the right thing." PMID:17348169

  7. Institutional Indicators: Proposed Budget, 1985-1986. Howard Community College.

    ERIC Educational Resources Information Center

    Radcliffe, Susan K.

    This report provides institutional indicators for budget projections based on data on student characteristics, credit instruction and degrees, credit-free instruction, faculty, community use of facilities, and fiscal background for fiscal years (FY) 1980 through 1984. Part 1 provides data on enrollments by program type, gender, minority status,…

  8. Peter Howard Elworthy (1933-1995): a biographical note.

    PubMed

    Florence, A T

    1997-06-01

    Peter Elworthy had a considerable influence on pharmaceutical science, education and practice, in the UK. He died in December 1995 at the early age of 62, but he had retired from full time academic work from his post as Professor of Pharmacy and Head of the Department of Pharmacy in the University of Manchester twelve years earlier. Concerned about his health but also not a little disillusioned by the multiple pressures placed even then on senior academics, he foresaw the era of cuts and the central oversight and restrictions. His heart was elsewhere. In his 1976 Harrison Memorial Lecture (reproduced in this issue) he said: "Looking back, I have the feeling of having been very lucky. 'Much have I travell'd in the realms of gold, and many goodly states and kingdoms seen.' My main interest has been in the phenomenon of micellization. The subject has been of absorbing interest, and continually shows new bright facets which lead to new scientific advances. Travelling in the realms of gold has nothing to do with gold, but to me it means travelling in sunlight, which illuminates things brightly, and makes visible new facts, which have been invisible before. Occasionally dark clouds form; they are the disappointments and frustrations, but we need them in order to be able to recognize the sunlight by contrast." PMID:9330193

  9. [Activities of the Department of Electrical Engineering, Howard University

    NASA Technical Reports Server (NTRS)

    Yalamanchili, Raj C.

    1997-01-01

    Theoretical derivations, computer analysis and test data are provided to demonstrate that the cavity model is a feasible one to analyze thin-substrate, rectangular-patch microstrip antennas. Seven separate antennas were tested. Most of the antennas were designed to resonate at L-band frequencies (1-2 GHz). One antenna was designed to resonate at an S-band (2-4 GHz) frequency of 2.025 GHz. All dielectric substrates were made of Duroid, and were of varying thicknesses and relative dielectric constant values. Theoretical derivations to calculate radiated free space electromagnetic fields and antenna input impedance were performed. MATHEMATICA 2.2 software was used to generate Smith Chart input impedance plots, normalized relative power radiation plots and to perform other numerical manipulations. Network Analyzer tests were used to verify the data from the computer programming (such as input impedance and VSWR). Finally, tests were performed in an anechoic chamber to measure receive-mode polar power patterns in the E and H planes. Agreement between computer analysis and test data is presented. The antenna with the thickest substrate (e(sub r) = 2.33,62 mils thick) showed the worst match to theoretical impedance data. This is anticipated due to the fact that the cavity model generally loses accuracy when the dielectric substrate thickness exceeds 5% of the antenna's free space wavelength. A method of reducing computer execution time for impedance calculations is also presented.

  10. NASA/Howard University Large Space Structures Institute

    NASA Technical Reports Server (NTRS)

    Broome, T. H., Jr.

    1984-01-01

    Basic research on the engineering behavior of large space structures is presented. Methods of structural analysis, control, and optimization of large flexible systems are examined. Topics of investigation include the Load Correction Method (LCM) modeling technique, stabilization of flexible bodies by feedback control, mathematical refinement of analysis equations, optimization of the design of structural components, deployment dynamics, and the use of microprocessors in attitude and shape control of large space structures. Information on key personnel, budgeting, support plans and conferences is included.

  11. Dale Avenue School Early Childhood Education Center Project. Research Bulletin, Volume I, No 1, December 1970.

    ERIC Educational Resources Information Center

    Paterson Board of Education, NJ.

    Published prior to the 1970 implementation of a Paterson, New Jersey, Title III early childhood program for 120 pre-kindergarten and 120 kindergarten aged disadvantaged children, the bulletin describes the school and student population, the evaluation procedure, differences between experimental and control groups, results of pretesting, the eight…

  12. Conversations with Four Highly Productive Educational Psychologists: Patricia Alexander, Richard Mayer, Dale Schunk, and Barry Zimmerman

    ERIC Educational Resources Information Center

    Patterson-Hazley, Melissa; Kiewra, Kenneth A.

    2013-01-01

    This article seeks to answer the questions: Who are the most productive and influential educational psychologists? What factors characterize these educational psychologists? And, what advice might they pass along to budding scholars? To determine the top educational psychologists, we surveyed the membership of Division 15 (Educational Psychology)…

  13. "13 Angry Men": Dale Bumper's "Ad Hominem" Impeachment Trial of President Clinton.

    ERIC Educational Resources Information Center

    Brovero, Adrienne F.

    2000-01-01

    Analyzes Senator Bumpers' argument in the Clinton impeachment proceedings as a bias type of "ad hominem" argument. Notes specifically Bumpers' argument that the House Managers were not fulfilling their proper roles in the proceedings. Claims Bumpers' bias position was reasonable in the argumentative context in which it took place. (NH)

  14. 76 FR 53964 - Dale J. Bingham, P.A.; Revocation of Registration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-30

    ... registration. See David W. Wang, 72 FR 54297, 54298 (2007); Sheran Arden Yeates, 71 FR 39130, 39131 (2006); Dominick A. Ricci, 58 FR 51104, 51105 (1993); Bobby Watts, 53 FR 11919, 11920 (1988). Here, while...'' and ``Requires PO Box Number.'' GX 3. On March 8, 2011, the Government served the Show Cause Order...

  15. Health-Hazard Evaluation Report HETA 85-195-1768, Dale Electronics, Incorporated, Norfolk, Nebraska

    SciTech Connect

    Lee, S.A.

    1987-01-01

    An evaluation was made of exposure to butyl cellosolve, epichlorohydrin, nickel, chromium, antimony, and total particulates for workers in an electronic resistor manufacturing process. No exposure to butyl cellosolve was detected among liquid coaters and no epichlorohydrin was detected in the process area air samples. No exposure to nickel or chromium was detected among mechanical-grinding spirallers. One laser spiraller was exposed to 8 micrograms/m/sup 3/ of nickel and 8 microg/m/sup 3/ chromium. Trace amounts of nickel were noted in the breathing zones of one plating lab worker and one calibrator. Total particulate exposures ranged from 0.1 to 0.9 mg/m/sup 3/. The author concludes that there were no known overexposures to contaminants at this facility and recommends further checking.

  16. STS-8 onboard crew press conference

    NASA Technical Reports Server (NTRS)

    1983-01-01

    Six news reporters listen to a response from Astronaut Guion S. Bluford (note TV monitor) in a rare space-to-Earth press conference involving all the STS-8 crew. The participants are, left to right, Gary Schwitzer, Cable News Network; Morton Dean, CBS; Roy Neal, NBC; Lynn Sherr, ABC; Howard Benedict, Associated Press; Al Rossiter, United Press International. The astronauts on the monitor are Richard H. Truly, cneter left, crew commander; Daniel C. Brandenstein, lower left, pilot; and Dr. William E. Thornton, upper left, Guion S. Bluford, upper right; and Dale E. Gardner, all mission specialists.

  17. PAT: an intelligent authoring tool for facilitating clinical trial design.

    PubMed

    Tagaris, Anastasios; Andronikou, Vassiliki; Karanastasis, Efstathios; Chondrogiannis, Efthymios; Tsirmpas, Charalambos; Varvarigou, Theodora; Koutsouris, Dimitris

    2014-01-01

    Great investments are made by both private and public funds and a wealth of research findings is published, the research and development pipeline phases quite low productivity and tremendous delays. In this paper, we present a novel authoring tool which has been designed and developed for facilitating study design. Its underlying models are based on a thorough analysis of existing clinical trial protocols (CTPs) and eligibility criteria (EC) published in clinicaltrials.gov by domain experts. Moreover, its integration with intelligent decision support services and mechanisms linking the study design process with healthcare patient data as well as its direct access to literature designate it as a powerful tool offering great support to researchers during clinical trial design. PMID:25160332

  18. Diagnostic models based on personalized analysis of trends (PAT).

    PubMed

    Hudson, Donna L; Cohen, Maurice E

    2010-07-01

    Many changes have taken place in medicine over the last century. In the first-half of the 20th century physicians were faced with the challenge of making diagnoses with too little information, often resorting to exploratory surgery to confirm the presence or absence of a condition. Due to rapid technological advances during the second-half of the 20th century, and continuing to this day, the position of the physician has now shifted from an information-poor environment to an environment with too much information, often exceeding the limits of human decision-making capabilities. To take full advantage of all available information, a new approach based on refined automated decision support methods is needed to assist the physician in the decision-making process. Medical decision support systems need to evolve from stand-alone systems to cooperative systems in which the physician becomes the decision maker, but relies on the decision support system to sift through information to determine relevant trends. In this paper, a decision support system that combines a number of methodologies for trend analysis is described, along with examples in cardiology. The methods have also been used in applications in neurology as well as cancer diagnosis and prognosis. PMID:19775973

  19. ML-PAT. Mohawk Language Picture Association Test.

    ERIC Educational Resources Information Center

    Cole, Glory; And Others

    This picture association test booklet for the Mohawk language has two parts. Part One contains 10 questions and Part Two contains 40 questions. Each item consists of a word in Mohawk and a number of drawing from which the learner is to choose the correct one that represents the word. (AMH)

  20. Synthesis and biological evaluation of gallium polyaminothiols (PAT).

    PubMed

    John, C S; Kinuya, S; Minear, G; Keast, R K; Paik, C H

    1993-02-01

    Two polyaminothiol ligands, one hexadentate, N,N',N"-tris[2-methyl(2-propanethiol)]-1,4,7-triazacyclononane (TACN), and another potentially heptadentate, tris[2-methyl(2-propanethiol)]aminoethylamine (TMAE), were synthesized and characterized using spectroscopic and analytical methods. Both ligands were labeled with gallium-67 at pH 3.0-3.5 in high yields. The resulting gallium chelates were lipophilic. The biodistribution studies for both the chelates showed hepatic uptake and biliary clearance. The total % ID activity for [67Ga]TACN in liver and intestine at 5 min was 33.86 increasing gradually to 61.4% at 30 min. The chelates were in vivo stable to plasma-transferrin transchelation as confirmed by scintigraphic images obtained by [67Ga]TACN in rats and by plasma incubation of the chelates. These results indicate that Ga-TACN is a potentially useful tracer for hepatobiliary imaging using PET. No brain uptake was found. PMID:8448576

  1. It's All about the Money: Chris and Pat Compare Salaries

    ERIC Educational Resources Information Center

    Renard, Monika K.

    2008-01-01

    Can you name 20 influences on pay that could cause a difference in earnings between two ostensibly equal employees? This short, involving exercise can be used to illustrate the numerous influences that affect how employees' pay is determined, for example, education, experience required for the job, supply and demand, company size, seniority, and…

  2. Compliance Monitoring of Juvenile Yearling Chinook Salmon and Steelhead Survival and Passage at The Dales Dam, Spring 2011

    SciTech Connect

    Skalski, John R.; Townsend, Richard L.; Seaburg, Adam; Johnson, Gary E.; Ploskey, Gene R.; Carlson, Thomas J.

    2012-02-01

    The study estimated dam passage survival at The Dalles Dam as stipulated by the 2008 Federal Columbia River Power System (FCRPS) Biological Opinion (BiOp) and provided additional performance measures as stipulated in the Columbia Basin Fish Accords. This summary report focuses on spring run stocks, yearling Chinook salmon and steelhead.

  3. 75 FR 18826 - Mr. Howard Rosenfeld; Notice of Preliminary Permit Application Accepted for Filing and Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-13

    ... proposed project would consist of: (1) An existing 80- foot-long, 4-foot-wide, and 5-foot-high masonry dam..., approximately 535-foot-long penstock leading to; (4) an existing 25-foot-high, 22-foot-square...

  4. Howard P. "Buck" McKeon National Defense Authorization Act for Fiscal Year 2015

    THOMAS, 113th Congress

    Rep. McKeon, Howard P. "Buck" [R-CA-25

    2014-04-09

    06/05/2014 Received in the Senate. Read twice. Placed on Senate Legislative Calendar under General Orders. Calendar No. 425. (All Actions) Notes: For further action, see H.R.3979, which became Public Law 113-291 on 12/19/2014. Tracker: This bill has the status Passed HouseHere are the steps for Status of Legislation:

  5. 77 FR 29456 - Central Railroad Company of Indianapolis-Abandonment Exemption-in Howard County, IN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-17

    ... partial revocation under 49 U.S.C. 10502(d) must be filed. Provided no formal expression of intent to file an offer of financial assistance (OFA) has been received, this exemption will be effective on June...

  6. Howard County Public Schools Social Studies Curriculum Unit: Middle School Human Relations.

    ERIC Educational Resources Information Center

    Howard County Board of Education, Clarksville, MD.

    Part of the rationale underlying this three-part human relations unit (taken from Major Concepts for Social Studies by the Social Studies Curriculum Center at Syracuse) is that students should become aware of the importance of the dignity of each individual, and the young citizen should be striving to work and cooperate with fellow individuals.…

  7. Lucy Diggs Slowe, Howard University Dean of Women, 1922-1937: Educator Administrator, Activist

    ERIC Educational Resources Information Center

    Rasheed, Lisa R.

    2010-01-01

    Within the last twenty years, some educational researchers initiated an emphasis to study the accomplishments and contributions of African-American women in higher education. Although they were marginally recognized, some African-American women forged into uncharted territories by providing examples of administrative leadership in post-secondary…

  8. Grubb, Thomas (1800-78) and Grubb, Howard (1844-1931)

    NASA Astrophysics Data System (ADS)

    Murdin, P.

    2000-11-01

    Irish telescope makers, based in Dublin, then in St Albans, England. Thomas was born in Waterford and set up a machinery business. After making a 9 in telescope for himself, he made many giant reflecting telescopes commercially. One of his first contracts was for a Cassegrain reflector for ROMNEY ROBINSON of Armagh Observatory, which introduced mechanical design features of significance for the f...

  9. Howard Zinn and the Struggle for the Microphone: History, Objectivity, and Citizenship

    ERIC Educational Resources Information Center

    Kelly, Jason M.

    2009-01-01

    Every year, historians in the United States attend the American Historical Association (AHA), a conference that has met annually since 1884. The AHA draws scholars from all specializations, and it is the primary organization through which the profession is represented. In 1969, the conference met at the Sheraton Park Hotel in Washington, D.C. At…

  10. The CEO as organizational architect: an interview with Xerox's Paul Allaire. Interview by Robert Howard.

    PubMed

    Allaire, P

    1992-01-01

    As chairman and CEO of the Xerox Corporation, Paul Allaire leads a company that is a microcosm of the changes transforming American business. With the introduction of the first plain-paper copier in 1959, Xerox invented a new industry and launched itself on a decade of spectacular growth. But easy growth led Xerox to neglect the fundamentals of its core business, leaving the company vulnerable to low-cost Japanese competition. Starting in the mid-1980s, Xerox embarked on a long-term effort to regain its dominant position in world copier markets and to create a new platform for future growth. Thanks to the company's Leadership through Quality program, Xerox became the first major U.S. company to win back market share from the Japanese. Allaire describes his efforts to take Xerox's corporate transformation to a new level. Since becoming CEO in 1990, he has repositioned Xerox as "the document company" at the intersection of the worlds of paper-based and electronic information. And he has guided the company through a fundamental redesign of what he calls the "organizational architecture" of Xerox's document processing business. Few CEOs have approached the process of organizational redesign as systematically and methodically as Allaire has. He has created a new corporate structure that balances independent business divisions with integrated R&D and customer operations organizations. He has redefined managerial roles and responsibilities, changed the way managers are selected and compensated, and renewed the company's senior management ranks. And he has articulated the new values and behaviors Xerox managers will need to thrive in a more competitive and fast-changing business environment. PMID:10121313

  11. Howard Gardner, Meet Benjamin Bloom: Strategies for the Future Enliven Methods from the Past.

    ERIC Educational Resources Information Center

    Savitz, Fred

    Classic theories in pedagogy such as those of John Dewey and Jean Piaget establish the foundation upon which preservice students in a social studies methods course build new knowledge about the teaching/learning paradigm. The constructivist philosophy emanating from these theories provides the rationale for a technique designed to enable students…

  12. "One Long Struggle for Justice": An Interview with Historian Howard Zinn

    ERIC Educational Resources Information Center

    Bigelow, Bill

    2010-01-01

    In 2008 "Rethinking Schools" and the Washington, D.C.-based education nonprofit Teaching for Change joined together to form the Zinn Education Project, dedicated to promoting the teaching of a people's history in middle and high schools throughout the United States. The Zinn Education Project recently launched a new website, www.zinnedproject.org,…

  13. Carl Levin and Howard P. "Buck" McKeon National Defense Authorization Act for Fiscal Year 2015

    THOMAS, 113th Congress

    Rep. Barletta, Lou [R-PA-11

    2014-01-31

    12/19/2014 Became Public Law No: 113-291. (TXT | PDF) (All Actions) Notes: See the committee print, containing the legislative text and joint explanatory statement, accompanying the enrolled version of H.R.3979. Tracker: This bill has the status Became LawHere are the steps for Status of Legislation:

  14. A resolution relative to the death of Howard O. Greene, Jr., former Sergeant at Arms of the United States Senate.

    THOMAS, 113th Congress

    Sen. McConnell, Mitch [R-KY

    2014-11-17

    11/17/2014 Submitted in the Senate, considered, and agreed to without amendment and with a preamble by Unanimous Consent. (consideration: CR S6005; text as passed Senate: CR S6024) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  15. 77 FR 76457 - Howard Hughes Medical Institute, et al.; Notice of Consolidated Decision on Applications for Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-28

    .... Manufacturer: SPECS Surface Nano Analysis, GmbH, Germany. Intended Use: See notice at 77 FR 70141-42. Comments.... Manufacturer: TTP Labtech Ltd., United Kingdom. Intended Use: See notice at 77 FR 70141, November 23, 2012... Use: See notice at 77 FR 70141, November 23, 2012. Comments: None received. Decision: Approved....

  16. Antinociceptive and anti-inflammatory effects of a lectin-like substance from Clitoria fairchildiana R. Howard seeds.

    PubMed

    Leite, Joana Filomena Magalhães; Assreuy, Ana Maria Sampaio; Mota, Mário Rogério Lima; Bringel, Pedro Henrique de Souza Ferreira; Lacerda, Rodrigo Rodrigues e; Gomes, Vinícius de Morais; Cajazeiras, João Batista; Nascimento, Kyria Santiago do; Pessôa, Hilzeth de Luna Freire; Gadelha, Carlos Alberto de Almeida; Delatorre, Plinio; Cavada, Benildo Sousa; Santi-Gadelha, Tatiane

    2012-01-01

    Lectins are proteins that have the ability to bind specifically and reversibly to carbohydrates and glycoconjugates, without altering the structure of the glycosyl ligand. They are found in organisms such as viruses, plants and humans, and they have been shown to possess important biological activities. The objective of this study was to purify and characterize lectins in the seeds of Clitoria fairchildiana, as well as to verify their biological activities. The results indicated the presence of a lectin (CFAL) in the glutelin acid protein fraction, which agglutinated native rabbit erythrocytes. CFAL was purified by column chromatography ion-exchange, DEAE-Sephacel, which was obtained from a peak of protein retained in the matrix by applying 0.5 M NaCl using the step-wise method. Electrophoretic analysis of this lectin in SDS-PAGE indicated a two band pattern protein molecular mass of approximately 100 and 116 kDa. CFAL proved to be unspecific to all carbohydrates/glycoconjugates in common use for the sugar inhibition test. This lectin showed no significant cytotoxicity to human red blood cells. It was observed that CFAL has anti-inflammatory activity in the paw edema induced by carrageenan model, in which a 64% diminution in edema was observed. Antinociceptive effects were observed for CFAL in the abdominal writhing test (induced by acetic acid), in which increasing doses of the lectin caused reduction in the number of contortions by up to 72%. It was concluded that the purified and characterized lectin from the seeds of Clitoria fairchildiana has anti-inflammatory and antinociceptive activity, and is not cytotoxic to human erythrocytes. PMID:22418929

  17. 77 FR 20009 - Howard Hughes Medical Institute, et al.; Notice of Consolidated Decision on Applications for Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-03

    ... notice at 77 FR 12240, February 29, 2012. Docket Number: 12-004. Applicant: Max Planck Florida Institute...: Electron Microscope. Manufacturer: FEI Company, Czech Republic. Intended Use: See notice at 77 FR 12240.... Intended Use: See notice at 77 FR 12240, February 29, 2012. Docket Number: 12-005. Applicant: VA Palo...

  18. 78 FR 35659 - Norfolk Southern Railway Company-Discontinuance of Service Exemption-in Tipton and Howard...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-13

    ... discontinue service over an approximately 10.8-mile rail line between milepost I-41.0 (north of Market Road near Tipton) and milepost ] I-51.8 (near the intersection of E. Lincoln Road and Home Avenue, in Kokomo... 49 CFR 1105.8(b), respectively. A copy of any petition filed with the Board should be sent to...

  19. 75 FR 63166 - Howard Rosenfeld; Notice of Declaration of Intention and Soliciting Comments, Protests, and/or...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-14

    ..., Protests, and/or Motions To Intervene October 6, 2010. Take notice that the following application has been... for filing comments, protests, and/or motions: November 08, 2010. All documents should be filed..., protests, and/or motions filed. k. Description of Project: The proposed Warren Energy...

  20. Is She Patting Katie? Constraints on Pronominal Reference in 30-Month-Olds

    ERIC Educational Resources Information Center

    Lukyanenko, Cynthia; Conroy, Anastasia; Lidz, Jeffrey

    2014-01-01

    In this study we investigate young children's knowledge of syntactic constraints on Noun Phrase reference by testing 30-month-olds' interpretation of two types of transitive sentences. In a preferential looking task, we find that children prefer different interpretations for transitive sentences whose object NP is a name (e.g.,…

  1. Dung pat nesting by the solitary bee, Osmia (Acanthosmiodes) integra (Megachilidae: Apiformes).

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Solitary bees nest in a diversity of substrates, typically soil, but also wood, stems and twigs. A new and novel substrate is reported here, dried cattle dung. Two species of Osmia bees were found nesting in dung in Wyoming. One species, O. integra, otherwise nests shallowly in soil. Nests were ...

  2. SURVIVAL OF ESCHERICHIA COLI IN COW PATS IN PASTURE AND IN LABORATORY CONDITIONS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Aims: To compare survival of E. coli in bovine feces deposited in a pasture or incubated in a controlled laboratory environment at comparable temperature regimes. Methods and Results: Fecal samples were collected from three cow herds on different diets. Samples were deposited as shaded and non-sha...

  3. Design and Preliminary Testing of a High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James; Meyer, Kirby; Kramer, Kevin; Smith, Gerald; Lewis, Raymond; Rodgers, Stephen L. (Technical Monitor)

    2000-01-01

    Antimatter represents the pinnacle of energy density, offering the potential to enhance current fusion/fission concepts enabling various classes of deep space missions. Current production rates are sufficient to support proof-of-concept evaluation of many key technologies associated with antimatter-derived propulsion. Storage has been identified as a key enabling technology for all antimatter-related operations, and as such is the current focus of this NASA-MSFC effort to design and fabricate a portable device capable of holding up to 10(exp 12) particles. Hardware has been assembled and initial tests are underway to evaluate the trap behavior using electron gun generated, positive hydrogen ions. Ions have been stored for tens of minutes, limited by observed interaction with background gas. Additionally, radio frequency manipulation is being tested to increase lifetime by stabilizing the stored particles, potentially reducing their interaction with background gas, easing requirements on ultimate trap vacuum and precision mechanical alignment.

  4. In Texas, Transfer Students Get an Extra Pat on the Back

    ERIC Educational Resources Information Center

    Mangan, Katherine

    2009-01-01

    In Texas, as in other states, fewer than a quarter of the students earning associate degrees end up applying to universities. The eventual result is widespread underemployment and a stagnating work force that doesn't keep up with population growth. However, Texas educators are trying to change that with two ambitious programs--one aimed at college…

  5. RF Manipulation and Detection of Protons in the High Performance Antiproton Trap (HiPAT)

    NASA Technical Reports Server (NTRS)

    Martin, James J.; Lewis, Raymond A.; Pearson, J. Boise; Sims, W. Herb; Chakrabarti, Suman; Fant, Wallace E.; McDonald, Stan

    2003-01-01

    The significant energy density of matter-antimatter annihilation is attractive to the designers of future space propulsion systems, with the potential to offer a highly compact source of power. Many propulsion concepts exist that could take advantage of matter-antimatter reactions, and current antiproton production rates are sufficient to support basic proof-of-principle evaluation of technology associated with antimatter-derived propulsion. One enabling technology for such experiments is portable storage of low energy antiprotons, allowing antiprotons to be trapped, stored, and transported for use at an experimental facility.

  6. 40 CFR 174.522 - Phosphinothricin Acetyltransferase (PAT); exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) enzyme are exempt from the requirement of a tolerance when used as plant-incorporated protectant inert... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PROCEDURES AND REQUIREMENTS FOR...

  7. 40 CFR 174.522 - Phosphinothricin Acetyltransferase (PAT); exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) enzyme are exempt from the requirement of a tolerance when used as plant-incorporated protectant inert... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PROCEDURES AND REQUIREMENTS FOR...

  8. 40 CFR 174.522 - Phosphinothricin Acetyltransferase (PAT); exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...) enzyme are exempt from the requirement of a tolerance when used as plant-incorporated protectant inert... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PROCEDURES AND REQUIREMENTS FOR...

  9. 40 CFR 174.522 - Phosphinothricin Acetyltransferase (PAT); exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) enzyme are exempt from the requirement of a tolerance when used as plant-incorporated protectant inert... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PROCEDURES AND REQUIREMENTS FOR...

  10. 40 CFR 174.522 - Phosphinothricin Acetyltransferase (PAT); exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...) enzyme are exempt from the requirement of a tolerance when used as plant-incorporated protectant inert... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS PROCEDURES AND REQUIREMENTS FOR...

  11. How Early Events Affect Growing Brains. An Interview with Neuroscientist Pat Levitt

    ERIC Educational Resources Information Center

    National Scientific Council on the Developing Child, 2006

    2006-01-01

    Recent advances in neuroscience show clearly how experience can change brain neurochemicals, and how this in turn affects the way the brain functions. As a result, early negative events actually get built into the growing brain's neurochemistry, altering the brain's architecture. Research is continuing to investigate how children with genetic…

  12. A PAT-based qualification of pharmaceutical excipients produced by batch or continuous processing.

    PubMed

    Hertrampf, A; Müller, H; Menezes, J C; Herdling, T

    2015-10-10

    Pharmaceutical excipients have an influence on the main requirements for medicinal products (viz., quality, safety and efficacy) but also on their manufacturability. During product lifecycle it may become necessary to introduce minor changes (e.g., to continuously improve it) or major changes in the validated process (e.g., moving it to a new production site, replacing process version or even disruptively changing processing type). Those changes can influence the critical to quality attributes of the product. Therefore, it is important to enhance process understanding to avoid the risk of any significant quality changes. Process analytical technology can support better decision making and risk-management as required in quality by design - viz., by many pharmaceutical regulatory authorities. This study compares the quality of the pharmaceutical excipient sodium carbonate (anhydrous) produced either in a batch or a continuous process. For continuous processing two different production lines were available that differed on the dryer and crystallizer types used. Therefore their influence on critical to quality attributes of sodium carbonate was investigated for each of the three processing alternatives. The overall goal was to identify which of the continuous processes ensures a similar product quality to batch processing. Namely, changes on chemical and physical attributes of the product were investigated with Raman spectroscopy, laser diffraction and X-ray powder diffraction. Principal component analysis, a very common multivariate analysis technique, was applied to extract relevant information from small differences at multiple spectral regions from samples from each process type and from each analytical technique used. Changing processing from batch to continuous improved consistency of certain attributes (e.g., particle size distribution) but affected others. However, the increased process/product knowledge gained can lead to an enhanced control strategy and ensure a similar product quality is obtained from distinct process versions. PMID:26072012

  13. Screening of bacterial biocontrols against sapstain fungus (Lasiodiplodia theobromae Pat.) of rubberwood (Hevea brasiliensis Muell.Arg.).

    PubMed

    Sajitha, K L; Maria Florence, E J; Dev, Suma Arun

    2014-09-01

    Diverse bacterial biocontrol agents from various sources of aerobic composts against the sapstain fungus Lasiodiplodia theobromae in rubberwood (Hevea brasiliensis) were isolated, screened and identified by various morphological, biochemical and molecular techniques. The inhibitory effect of seventeen bacterial isolates was examined and seven exhibited inhibition towards the sapstain fungus. Among the seven antagonists, six were conclusively identified as Bacillus subtilis and one as Paenibacillus polymyxa using 16S rRNA-encoding gene sequencing. This is the first report on the occurrence of P. polymyxa, a potent biofertilizer and antagonist in vermicompost. HiCrome Bacillus agar was identified as an effective medium for differentiation of B. subtilis from other Bacillus species. The present work demonstrates the efficacy of the antagonistic property of B. subtilis strains against rubberwood sapstain fungus. Culture-based antagonistic inhibition displayed by B. subtilis can be extended to cater to the biocontrol requirements of wood-based industries against the stain fungus. The study showed the utility of an integrated approach, employing morphological, biochemical and molecular tools for conclusive identification of several bacterial isolates present in aerobic composts from diverse sources. PMID:25049165

  14. 'Don't save her'- Sigmund Freud meets Project Pat: the rescue motif in hip-hop.

    PubMed

    Fulmer, Richard H

    2008-08-01

    Freud originally explicated the dynamics of rescue wishes by describing men who fell in love with prostitutes. He saw this attempt at attachment as driven by the man's wish to repay his parents for giving him life. Many subsequent writers shift this emphasis, seeing rescue wishes as motivated by aggressive oedipal competition with the father. This article highlights the attachment aspects of Freud's original conception and traces how writers in the last three decades use the family romance rather than Oedipus as a model to view rescue wishes as having a more tender aspect. Rescue wishes are especially characteristic of the developmental stage of young adulthood. They at once attempt to repay the parental debt the young adult feels and serve as practice for the vicissitudes of the couple bond and the benign sacrifices of parenthood. Popular culture contains many vivid examples of Freud 's original description of the rescue-motif. A detailed examination of the rap song, Don't save her, demonstrates all the elements of Freud's original conception and the interpersonal risks of the rescue relationship. Three additional rap songs by other artists are briefly analyzed to show the extensive occurrence of the wish to rescue and its psychic and interpersonal dangers. PMID:18816338

  15. Decay of Fecal Indicator Bacterial Populations and Bovine-Associated Source-Tracking Markers in Freshly Deposited Cow Pats

    EPA Science Inventory

    Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about...

  16. Hybrid modeling as a QbD/PAT tool in process development: an industrial E. coli case study.

    PubMed

    von Stosch, Moritz; Hamelink, Jan-Martijn; Oliveira, Rui

    2016-05-01

    Process understanding is emphasized in the process analytical technology initiative and the quality by design paradigm to be essential for manufacturing of biopharmaceutical products with consistent high quality. A typical approach to developing a process understanding is applying a combination of design of experiments with statistical data analysis. Hybrid semi-parametric modeling is investigated as an alternative method to pure statistical data analysis. The hybrid model framework provides flexibility to select model complexity based on available data and knowledge. Here, a parametric dynamic bioreactor model is integrated with a nonparametric artificial neural network that describes biomass and product formation rates as function of varied fed-batch fermentation conditions for high cell density heterologous protein production with E. coli. Our model can accurately describe biomass growth and product formation across variations in induction temperature, pH and feed rates. The model indicates that while product expression rate is a function of early induction phase conditions, it is negatively impacted as productivity increases. This could correspond with physiological changes due to cytoplasmic product accumulation. Due to the dynamic nature of the model, rational process timing decisions can be made and the impact of temporal variations in process parameters on product formation and process performance can be assessed, which is central for process understanding. PMID:26879643

  17. Institutionalizing McCarthyism: An Exploration of Senator Pat McCarran's Anti-Communist Activities in Congress.

    ERIC Educational Resources Information Center

    Yarbrough, Charles G., Jr.

    Contending that Senator Patrick McCarran of Nevada was the real force behind the communist witch hunts of the lage 1940s and early 1950s, this essay explores McCarran's legislative legacy. The first section examines McCarran's background and the legislative bodies over which he held sway. The second section delineates his legislative "Grand…

  18. A one-dimensional, steady-state, dissolved-oxygen model and waste-load assimilation study for Wildcat Creek, Howard County, Indiana

    USGS Publications Warehouse

    Crawford, Charles G.; Wilber, William G.; Peters, James G.

    1979-01-01

    The Indiana State Board of Health is developing a water-quality management plan that includes establishing limits for wastewater effluents discharged into Indiana streams. A digital model calibrated to conditions in Wildcat Creek was used to predict alternatives for future waste loadings that would be compatible with Indiana stream water-quality standards defined for two critical hydrologic conditions, summer and winter low flows. The model indicates that benthic-oxygen demand is the most significant factor affecting the dissolved-oxygen concentrations in Wildcat Creek during summer low flows. The Indiana stream dissolved-oxygen standard should not be violated if the Kokomo wastewater-treatment facility meets its current National Pollution Discharge Elimination System permit restrictions (average monthly 5-day biochemical-oxygen demand of 5 milligrams per liter and maximum weekly 5-day biochemical-oxygen demand of 7.5 milligrams per liter) and benthic-oxygen demand becomes negligible. Ammonia-nitrogen toxicity may also be a water-quality limitation in Wildcat Creek. Ammonia-nitrogen waste loads for the Kokomo wastewater-treatment facility, projected by the Indiana State Board of Health, will result in stream ammonia-nitrogen concentrations that exceed the State standard (2.5 milligrams per liter during summer months and 4.0 milligrams per liter during winter months). (Kosco-USGS)

  19. Anthropophilic biting behaviour of Anopheles (Kerteszia) neivai Howard, Dyar & Knab associated with Fishermen’s activities in a malaria-endemic area in the Colombian Pacific

    PubMed Central

    Escovar, Jesús Eduardo; González, Ranulfo; Quiñones, Martha Lucía

    2013-01-01

    On the southwest Pacific Coast of Colombia, a field study was initiated to determine the human-vector association between Anopheles (Kerteszia) neivai and fishermen, including their nearby houses. Mosquitoes were collected over 24-h periods from mangrove swamps, marshlands and fishing vessels in three locations, as well as in and around the houses of fishermen. A total of 6,382 mosquitoes were collected. An. neivai was most abundant in mangroves and fishing canoes (90.8%), while Anopheles albimanus was found indoors (82%) and outdoors (73%). One An. neivai and one An. albimanus collected during fishing activities in canoes were positive for Plasmodium vivax , whereas one female An. neivai collected in a mangrove was positive for P. vivax . In the mangroves and fishing canoes, An. neivai demonstrated biting activity throughout the day, peaking between 06:00 pm-07:00 pm and there were two minor peaks at dusk and dawn. These peaks coincided with fishing activities in the marshlands and mangroves, a situation that places the fishermen at risk of contracting malaria when they are performing their daily activities. It is recommended that protective measures be implemented to reduce the risk that fishermen will contract malaria. PMID:24402159

  20. Aeromagnetic survey of Howard Pass quadrangle and the East half of Misheguk Mountain quadrangle, Alaska—a Web site for the distribution of data

    USGS Publications Warehouse

    Brown, Philip J.

    2009-01-01

    U.S. Geological Survey Open-File-Report 2009-1256 is for the preliminary release of magnetic data (and associated contractor reports) for an airborne survey in the Brooks Range, northwest of Bettles, Alaska.

  1. Anthropophilic biting behaviour of Anopheles (Kerteszia) neivai Howard, Dyar & Knab associated with Fishermen's activities in a malaria-endemic area in the Colombian Pacific.

    PubMed

    Escovar, Jesús Eduardo; González, Ranulfo; Quiñones, Martha Lucía

    2013-12-01

    On the southwest Pacific Coast of Colombia, a field study was initiated to determine the human-vector association between Anopheles (Kerteszia) neivai and fishermen, including their nearby houses. Mosquitoes were collected over 24-h periods from mangrove swamps, marshlands and fishing vessels in three locations, as well as in and around the houses of fishermen. A total of 6,382 mosquitoes were collected. An. neivai was most abundant in mangroves and fishing canoes (90.8%), while Anopheles albimanus was found indoors (82%) and outdoors (73%). One An. neivai and one An. albimanus collected during fishing activities in canoes were positive for Plasmodium vivax , whereas one female An. neivai collected in a mangrove was positive for P. vivax . In the mangroves and fishing canoes, An. neivai demonstrated biting activity throughout the day, peaking between 06:00 pm-07:00 pm and there were two minor peaks at dusk and dawn. These peaks coincided with fishing activities in the marshlands and mangroves, a situation that places the fishermen at risk of contracting malaria when they are performing their daily activities. It is recommended that protective measures be implemented to reduce the risk that fishermen will contract malaria. PMID:24402159

  2. President Franklin D Roosevelt (1882-1945) and Doctor Frank Howard Lahey's (1880-1953) dilemma: The complexities of medical confidentiality with World Leaders.

    PubMed

    Steinberg, David

    2016-02-01

    In the months before the Presidential Election of November 1944, there were serious concerns about the health of President Franklin D Roosevelt. He had lost considerable weight and his once robust appearance had given way to a tired and haggard look. Despite these worrisome observations, Vice-Admiral Ross T McIntire, who served as Franklin Roosevelt's personal physician from 1933 until Roosevelt's death, lied when he proclaimed Roosevelt to be in 'excellent condition for a man of his age'. The truth about Roosevelt's health was further obscured when Roosevelt's medical records disappeared. PMID:24737195

  3. Geologic and Fossil Locality Maps of the West-Central Part of the Howard Pass Quadrangle and Part of the Adjacent Misheguk Mountain Quadrangle, Western Brooks Range, Alaska

    USGS Publications Warehouse

    Dover, James H.; Tailleur, Irvin L.; Dumoulin, Julie A.

    2004-01-01

    The map depicts the field distribution and contact relations between stratigraphic units, the tectonic relations between major stratigraphic sequences, and the detailed internal structure of these sequences. The stratigraphic sequences formed in a variety of continental margin depositional environments, and subsequently underwent a complexde formational history of imbricate thrust faulting and folding. A compilation of micro and macro fossil identifications is included in this data set.

  4. Scientific analysis is essential to assess biofuel policy effects: in response to the paper by Kim and Dale on "Indirect land use change for biofuels: Testing predictions and improving analytical methodologies"

    SciTech Connect

    Kline, Keith L; Oladosu, Gbadebo A; Dale, Virginia H; McBride, Allen

    2011-01-01

    Vigorous debate on the effects of biofuels derives largely from the changes in land use estimated using economic models designed mainly for the analysis of agricultural trade and markets. The models referenced for land-use change (LUC) analysis in the U.S. Environmental Protection Agency Final Rule on the Renewable Fuel Standard include GTAP, FAPRI-CARD, and FASOM. To address bioenergy impacts, these models were expanded and modified to facilitate simulations of hypothesized LUC. However, even when models use similar basic assumptions and data, the range of LUC results can vary by ten-fold or more. While the market dynamics simulated in these models include processes that are important in estimating effects of biofuel policies, the models have not been validated for estimating land-use changes and employ crucial assumptions and simplifications that contradict empirical evidence.

  5. 12. UNIDENTIFIED CABLESTAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TWELVE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. UNIDENTIFIED CABLE-STAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TWELVE PANELS, SHOWING CABLE PATTERN SIMILAR TO E.E. RUNYON'S SUSPENSION BRIDGE PATENTS. THE BLUFF DALE SUSPENSION BRIDGE'S CABLES MAY HAVE ORIGINALLY FOLLOWED THIS PATTERN. 3/4 VIEW FROM ABOVE. - Bluff Dale Suspension Bridge, Spanning Paluxy River at County Route 149, Bluff Dale, Erath County, TX

  6. 13. UNIDENTIFIED CABLESTAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TWELVE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. UNIDENTIFIED CABLE-STAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TWELVE PANELS, SHOWING CABLE PATTERN SIMILAR TO E.E. RUNYON'S SUSPENSION BRIDGE PATENTS. THE BLUFF DALE SUSPENSION BRIDGE'S CABLES MAY HAVE ORIGINALLY FOLLOWED THIS PATTERN. ELEVATION VIEW. - Bluff Dale Suspension Bridge, Spanning Paluxy River at County Route 149, Bluff Dale, Erath County, TX

  7. 78 FR 13883 - Federal Property Suitable as Facilities To Assist the Homeless

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-01

    ... needed; asbestos; w/in controlled area; contact Ranger District for accessibility Dale Duplex (1057...; major repairs needed; asbestos Dale Residence (1059) 48743 Hwy 395 N. Dale OR 97880 Landholding Agency...; major repairs needed; asbestos; w/in controlled area; contact Ranger District for accessibility...

  8. Preparation of Power Distribution System for High Penetration of Renewable Energy Part I. Dynamic Voltage Restorer for Voltage Regulation Pat II. Distribution Circuit Modeling and Validation

    NASA Astrophysics Data System (ADS)

    Khoshkbar Sadigh, Arash

    Part I: Dynamic Voltage Restorer In the present power grids, voltage sags are recognized as a serious threat and a frequently occurring power-quality problem and have costly consequence such as sensitive loads tripping and production loss. Consequently, the demand for high power quality and voltage stability becomes a pressing issue. Dynamic voltage restorer (DVR), as a custom power device, is more effective and direct solutions for "restoring" the quality of voltage at its load-side terminals when the quality of voltage at its source-side terminals is disturbed. In the first part of this thesis, a DVR configuration with no need of bulky dc link capacitor or energy storage is proposed. This fact causes to reduce the size of the DVR and increase the reliability of the circuit. In addition, the proposed DVR topology is based on high-frequency isolation transformer resulting in the size reduction of transformer. The proposed DVR circuit, which is suitable for both low- and medium-voltage applications, is based on dc-ac converters connected in series to split the main dc link between the inputs of dc-ac converters. This feature makes it possible to use modular dc-ac converters and utilize low-voltage components in these converters whenever it is required to use DVR in medium-voltage application. The proposed configuration is tested under different conditions of load power factor and grid voltage harmonic. It has been shown that proposed DVR can compensate the voltage sag effectively and protect the sensitive loads. Following the proposition of the DVR topology, a fundamental voltage amplitude detection method which is applicable in both single/three-phase systems for DVR applications is proposed. The advantages of proposed method include application in distorted power grid with no need of any low-pass filter, precise and reliable detection, simple computation and implementation without using a phased locked loop and lookup table. The proposed method has been verified by simulation and experimental tests under various conditions considering all possible cases such as different amounts of voltage sag depth (VSD), different amounts of point-on-wave (POW) at which voltage sag occurs, harmonic distortion, line frequency variation, and phase jump (PJ). Furthermore, the ripple amount of fundamental voltage amplitude calculated by the proposed method and its error is analyzed considering the line frequency variation together with harmonic distortion. The best and worst detection time of proposed method were measured 1ms and 8.8ms, respectively. Finally, the proposed method has been compared with other voltage sag detection methods available in literature. Part 2: Power System Modeling for Renewable Energy Integration: As power distribution systems are evolving into more complex networks, electrical engineers have to rely on software tools to perform circuit analysis. There are dozens of powerful software tools available in the market to perform the power system studies. Although their main functions are similar, there are differences in features and formatting structures to suit specific applications. This creates challenges for transferring power system circuit models data (PSCMD) between different software and rebuilding the same circuit in the second software environment. The objective of this part of thesis is to develop a Unified Platform (UP) to facilitate transferring PSCMD among different software packages and relieve the challenges of the circuit model conversion process. UP uses a commonly available spreadsheet file with a defined format, for any home software to write data to and for any destination software to read data from, via a script-based application called PSCMD transfer application. The main considerations in developing the UP are to minimize manual intervention and import a one-line diagram into the destination software or export it from the source software, with all details to allow load flow, short circuit and other analyses. In this study, ETAP, OpenDSS, and GridLab-D are considered, and PSCMD trans

  9. Application of terahertz pulse imaging as PAT tool for non-destructive evaluation of film-coated tablets under different manufacturing conditions.

    PubMed

    Dohi, Masafumi; Momose, Wataru; Yoshino, Hiroyuki; Hara, Yuko; Yamashita, Kazunari; Hakomori, Tadashi; Sato, Shusaku; Terada, Katsuhide

    2016-02-01

    Film-coated tablets (FCTs) are a popular solid dosage form in pharmaceutical industry. Manufacturing conditions during the film-coating process affect the properties of the film layer, which might result in critical quality problems. Here, we analyzed the properties of the film layer using a non-destructive approach with terahertz pulsed imaging (TPI). Hydrophilic tablets that become distended upon water absorption were used as core tablets and coated with film under different manufacturing conditions. TPI-derived parameters such as film thickness (FT), film surface reflectance (FSR), and interface density difference (IDD) between the film layer and core tablet were affected by manufacturing conditions and influenced critical quality attributes of FCTs. Relative standard deviation of FSR within tablets correlated well with surface roughness. Tensile strength could be predicted in a non-destructive manner using the multivariate regression equation to estimate the core tablet density by film layer density and IDD. The absolute value of IDD (Lateral) correlated with the risk of cracking on the lateral film layer when stored in a high-humidity environment. Further, in-process control was proposed for this value during the film-coating process, which will enable a feedback control system to be applied to process parameters and reduced risk of cracking without a stability test. PMID:26678177

  10. Preparation of Power Distribution System for High Penetration of Renewable Energy Part I. Dynamic Voltage Restorer for Voltage Regulation Pat II. Distribution Circuit Modeling and Validation

    NASA Astrophysics Data System (ADS)

    Khoshkbar Sadigh, Arash

    Part I: Dynamic Voltage Restorer In the present power grids, voltage sags are recognized as a serious threat and a frequently occurring power-quality problem and have costly consequence such as sensitive loads tripping and production loss. Consequently, the demand for high power quality and voltage stability becomes a pressing issue. Dynamic voltage restorer (DVR), as a custom power device, is more effective and direct solutions for "restoring" the quality of voltage at its load-side terminals when the quality of voltage at its source-side terminals is disturbed. In the first part of this thesis, a DVR configuration with no need of bulky dc link capacitor or energy storage is proposed. This fact causes to reduce the size of the DVR and increase the reliability of the circuit. In addition, the proposed DVR topology is based on high-frequency isolation transformer resulting in the size reduction of transformer. The proposed DVR circuit, which is suitable for both low- and medium-voltage applications, is based on dc-ac converters connected in series to split the main dc link between the inputs of dc-ac converters. This feature makes it possible to use modular dc-ac converters and utilize low-voltage components in these converters whenever it is required to use DVR in medium-voltage application. The proposed configuration is tested under different conditions of load power factor and grid voltage harmonic. It has been shown that proposed DVR can compensate the voltage sag effectively and protect the sensitive loads. Following the proposition of the DVR topology, a fundamental voltage amplitude detection method which is applicable in both single/three-phase systems for DVR applications is proposed. The advantages of proposed method include application in distorted power grid with no need of any low-pass filter, precise and reliable detection, simple computation and implementation without using a phased locked loop and lookup table. The proposed method has been verified by simulation and experimental tests under various conditions considering all possible cases such as different amounts of voltage sag depth (VSD), different amounts of point-on-wave (POW) at which voltage sag occurs, harmonic distortion, line frequency variation, and phase jump (PJ). Furthermore, the ripple amount of fundamental voltage amplitude calculated by the proposed method and its error is analyzed considering the line frequency variation together with harmonic distortion. The best and worst detection time of proposed method were measured 1ms and 8.8ms, respectively. Finally, the proposed method has been compared with other voltage sag detection methods available in literature. Part 2: Power System Modeling for Renewable Energy Integration: As power distribution systems are evolving into more complex networks, electrical engineers have to rely on software tools to perform circuit analysis. There are dozens of powerful software tools available in the market to perform the power system studies. Although their main functions are similar, there are differences in features and formatting structures to suit specific applications. This creates challenges for transferring power system circuit models data (PSCMD) between different software and rebuilding the same circuit in the second software environment. The objective of this part of thesis is to develop a Unified Platform (UP) to facilitate transferring PSCMD among different software packages and relieve the challenges of the circuit model conversion process. UP uses a commonly available spreadsheet file with a defined format, for any home software to write data to and for any destination software to read data from, via a script-based application called PSCMD transfer application. The main considerations in developing the UP are to minimize manual intervention and import a one-line diagram into the destination software or export it from the source software, with all details to allow load flow, short circuit and other analyses. In this study, ETAP, OpenDSS, and GridLab-D are considered, and PSCMD transfer applications written in MATLAB have been developed for each of these to read the circuit model data provided in the UP spreadsheet. In order to test the developed PSCMD transfer applications, circuit model data of a test circuit and a power distribution circuit from Southern California Edison (SCE) - a utility company - both built in CYME, were exported into the spreadsheet file according to the UP format. Thereafter, circuit model data were imported successfully from the spreadsheet files into above mentioned software using the PSCMD transfer applications developed for each software. After the SCE studied circuit is transferred into OpenDSS software using the proposed UP scheme and developed application, it has been studied to investigate the impacts of large-scale solar energy penetration. The main challenge of solar energy integration into power grid is its intermittency (i.e., discontinuity of output power) nature due to cloud shading of photovoltaic panels which depends on weather conditions. In order to conduct this study, OpenDSS time-series simulation feature, which is required due to intermittency of solar energy, is utilized. In this study, the impacts of intermittency of solar energy penetration, especially high-variability points, on voltage fluctuation and operation of capacitor bank and voltage regulator is provided. In addition, the necessity to interpolate and resample unequally spaced time-series measurement data and convert them to equally spaced time-series data as well as the effect of resampling time-interval on the amount of error is discussed. Two applications are developed in Matlab to do interpolation and resampling as well as to calculate the amount of error for different resampling time-intervals to figure out the suitable resampling time-interval. Furthermore, an approach based on cumulative distribution, regarding the length for lines/cables types and the power rating for loads, is presented to prioritize which loads, lines and cables the meters should be installed at to have the most effect on model validation.

  11. Real-time polymerase chain reaction-based approach for quantification of the pat gene in the T25 Zea mays event.

    PubMed

    Weighardt, Florian; Barbati, Cristina; Paoletti, Claudia; Querci, Maddalena; Kay, Simon; De Beuckeleer, Marc; Van den Eede, Guy

    2004-01-01

    In Europe, a growing interest for reliable techniques for the quantification of genetically modified component(s) of food matrixes is arising from the need to comply with the European legislative framework on novel food products. Real-time polymerase chain reaction (PCR) is currently the most powerful technique for the quantification of specific nucleic acid sequences. Several real-time PCR methodologies based on different molecular principles have been developed for this purpose. The most frequently used approach in the field of genetically modified organism (GMO) quantification in food or feed samples is based on the 5'-3'-exonuclease activity of Taq DNA polymerase on specific degradation probes (TaqMan principle). A novel approach was developed for the establishment of a TaqMan quantification system assessing GMO contents around the 1% threshold stipulated under European Union (EU) legislation for the labeling of food products. The Zea mays T25 elite event was chosen as a model for the development of the novel GMO quantification approach. The most innovative aspect of the system is represented by the use of sequences cloned in plasmids as reference standards. In the field of GMO quantification, plasmids are an easy to use, cheap, and reliable alternative to Certified Reference Materials (CRMs), which are only available for a few of the GMOs authorized in Europe, have a relatively high production cost, and require further processing to be suitable for analysis. Strengths and weaknesses of the use of novel plasmid-based standards are addressed in detail. In addition, the quantification system was designed to avoid the use of a reference gene (e.g., a single copy, species-specific gene) as normalizer, i.e., to perform a GMO quantification based on an absolute instead of a relative measurement. In fact, experimental evidences show that the use of reference genes adds variability to the measurement system because a second independent real-time PCR-based measurement must be performed. Moreover, for some reference genes no sufficient information on copy number in and among genomes of different lines is available, making adequate quantification difficult. Once developed, the method was subsequently validated according to IUPAC and ISO 5725 guidelines. Thirteen laboratories from 8 EU countries participated in the trial. Eleven laboratories provided results complying with the predefined study requirements. Repeatability (RSDr) values ranged from 8.7 to 15.9%, with a mean value of 12%. Reproducibility (RSDR) values ranged from 16.3 to 25.5%, with a mean value of 21%. Following Codex Alimentarius Committee guidelines, both the limits of detection and quantitation were determined to be <0.1%. PMID:15675446

  12. [Studies on the effects of carbon:nitrogen ratio, inoculum type and yeast extract addition on jasmonic acid production by Botryodiplodia theobromae Pat. strain RC1].

    PubMed

    Eng Sánchez, Felipe; Gutiérrez-Rojas, Mariano; Favela-Torres, Ernesto

    2008-09-30

    Jasmonic acid is a native plant growth regulator produced by algae, microorganisms and higher plants. This regulator is involved in the activation of defence mechanisms against pathogens and wounding in plants. Studies concerning the effects of carbon: nitrogen ratio (C/Nr: 17, 35 and 70), type of inoculum (spores or mycelium) and the yeast extract addition in the media on jasmonic acid production by Botryodiplodia theobromae were evaluated. Jasmonic acid production was stimulated at the carbon: nitrogen ratio of 17. Jasmonic acid productivity was higher in the media inoculated with mycelium and in the media with yeast extract 1.7 and 1.3 times, respectively. PMID:18785793

  13. Two Decades of Immigration: Has the Sky Fallen Yet? Pat Brown Institute of Public Affairs State of Immigration Report. Volume 1.

    ERIC Educational Resources Information Center

    Modarres, Ali

    This report provides a demographic analysis of immigration to the United States and California, using information from the Immigration and Naturalization Service annual reports and digital database for 1990-98. It features details on the Asian immigrant population for the 1990s. From 1820-1998, about 62 million immigrants came to America. Nearly…

  14. Ecology of Alcohol and Other Drug Use: Helping Black High-Risk Youth. Proceedings of the Howard University School of Human Ecology Forum (Washington, D.C., October 26-27, 1987). OSAP Prevention Monograph-7.

    ERIC Educational Resources Information Center

    Oyemade, Ura Jean, Ed.; Brandon-Monye, Deloris, Ed.

    Five plenary session presentations and summaries of 10 panel sessions held at a forum entitled "The Ecology of Substance Abuse: Toward Primary Prevention among High-Risk Youth" are provided in this document, which focuses on black youth at high risk for alcohol and drug problems. Experts describe a comprehensive ecological approach to addressing…

  15. Testimony by Susanne H. Howard, Associate Under Secretary for Economic Affairs, before the Subcommittee on Census and Population, Committee on Post Office and Civil Service, U.S. House of Representatives (Anaheim, California, August 8, 1990).

    ERIC Educational Resources Information Center

    Howard, Susanne H.

    Promotion, outreach, and data production efforts related to the 1990 decennial census conducted by the U.S. Department of Commerce are discussed. Promotional efforts, which particularly targeted minority groups, included multilingual mass media advertisements, public service announcements, press releases, school and church components, and…

  16. How HANDy Are Baby Signs? A Systematic Review of the Impact of Gestural Communication on Typically Developing, Hearing Infants under the Age of 36 Months: Response to Howard and Doherty-Sneddon's Commentary

    ERIC Educational Resources Information Center

    Fitzpatrick, Elizabeth M.; Johnston, J. Cyne; Thibert, Jonelle; Grandpierre, Viviane

    2014-01-01

    A systematic review was conducted to synthesize the evidence related to the effectiveness of baby sign language for children with typical development. This response to a Commentary on the review stresses that the primary purpose of the review was to assist caregivers and policy makers with informed decision-making related to the benefits of the…

  17. 37 CFR 102.34 - Specific exemptions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... are within this exemption are: COMMERCE/PAT-TM-6, COMMERCE/PAT-TM-7, COMMERCE/PAT-TM-8, COMMERCE/PAT-TM-9. (2) USPTO hereby asserts a claim to exemption of such materials wherever they might appear in.../DEPT-14; (D) Attorneys and Agents Registered to Practice Before the Office—COMMERCE/PAT-TM-1;...

  18. 24 CFR 3280.504 - Condensation control and installation of vapor retarders.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Bullock, Butler, Choctaw, Clarke, Coffee, Conecuh, Covington, Crenshaw, Dale, Escambia, Geneva, Henry..., Brooks, Bryan, Calhoun, Camden, Charlton, Chatham, Clay, Clinch, Coffee, Colquitt, Cook, Crisp,...

  19. 77 FR 22577 - Change in Bank Control Notices; Formations of, Acquisitions by, and Mergers of Bank Holding...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-16

    ...; Correction This notice corrects a notice (FR Doc. 2012-08198) published on page 20635 of the issue for...; Frederick S. Loomis, Anne M. Loomis, and J. Porter Loomis, all of Pratt, Kansas; Howard K. Loomis, Jr..., New York; Howard K. Loomis Revocable Trust, 2010 Howard K. Loomis Irrevocable Family Trust,...

  20. Mapping Me: Mapping Identity among Academically High-Performing Black Males

    ERIC Educational Resources Information Center

    Flennaugh, Terry K.

    2016-01-01

    Background: Scholars have argued that far too little research has examined the complex processes that many Black males undertake in constructing identities that function in schools (Howard & Flennaugh, 2011; Howard, Flennaugh, & Terry, 2012; Terry, Flennaugh, Blackmon, & Howard, 2014). Some have highlighted the perpetuation of a false…

  1. The Effectiveness of the Smog Index in Determining the Reading Levels of Business and Distributive Education Texts.

    ERIC Educational Resources Information Center

    Schultheis, Robert A.; Anderson, Roberta

    1982-01-01

    McLaughlin's Smog Index was compared to the Dale-Chall formula for the determination of reading levels of 48 textbooks in business and distributive education. A Modified Smog Index proved a valid substitute for the Dale-Chall formula when used to evaluate the reading levels of business and distributive education narrative. (Author/CT)

  2. KSC-03PD-0960

    NASA Technical Reports Server (NTRS)

    2003-01-01

    KENNEDY SPACE CENTER, FLA. - Ron Dale (left, foreground) is greeted by friends and co-workers on his return to KSC. Dale, with the NASA Safety and Process Assurance Branch at KSC, was aboard a helicopter that crashed while searching for debris from the Space Shuttle Columbia in east Texas in March. He was treated for nonlife-threatening injuries and recently released.

  3. 76 FR 76449 - Post Office Closing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-07

    ... Post Office Closing AGENCY: Postal Regulatory Commission. ACTION: Notice. SUMMARY: This document informs the public that an appeal of the closing of the Spring Dale, West Virginia post office has been... four petitions for review of the Postal Service's determination to close the Spring Dale post office...

  4. 14. UNIDENTIFIED CABLESTAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TEN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. UNIDENTIFIED CABLE-STAYED SUSPENSION BRIDGE WITH TIMBER RAILING OF TEN PANELS AND STONE PIER, SHOWING ELEMENTS SIMILAR TO E.E. RUNYON'S SUSPENSION BRIDGE PATENTS, BUT ALTERNATE CABLE PATTERN. 3/4 VIEW FROM BELOW. - Bluff Dale Suspension Bridge, Spanning Paluxy River at County Route 149, Bluff Dale, Erath County, TX

  5. French II: Terminal Course. A Summer Curriculum Project.

    ERIC Educational Resources Information Center

    Santmyers, Muriel H.

    A two-semester terminal course in French, based on a text entitled "Cours Elementaire" by Dale and Dale, is articulated in this curriculum guide. The purpose of the course, which is designed for students who are not interested in completing a three- or four-year program, course content, general plan, and teaching strategies are examined. A plan…

  6. 75 FR 145 - Clean Air Act Operating Permit Program; Petition for Objection to State Operating Permit for East...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-04

    ... regarding the EKPC Dale Station on November 24, 2008, requesting that EPA object to the title V operating... AGENCY Clean Air Act Operating Permit Program; Petition for Objection to State Operating Permit for East Kentucky Power Cooperative, Inc.-- William C. Dale Power Station; Clark County, KY AGENCY:...

  7. Adaptive Assessment of Student's Knowledge in Programming Courses

    ERIC Educational Resources Information Center

    Chatzopoulou, D. I.; Economides, A. A.

    2010-01-01

    This paper presents Programming Adaptive Testing (PAT), a Web-based adaptive testing system for assessing students' programming knowledge. PAT was used in two high school programming classes by 73 students. The question bank of PAT is composed of 443 questions. A question is classified in one out of three difficulty levels. In PAT, the levels of…

  8. 37 CFR 102.34 - Specific exemptions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .../DEPT-14; (D) Attorneys and Agents Registered to Practice Before the Office—COMMERCE/PAT-TM-1; (E... OF COMMERCE ADMINISTRATION DISCLOSURE OF GOVERNMENT INFORMATION Privacy Act § 102.34 Specific... are within this exemption are: COMMERCE/PAT-TM-6, COMMERCE/PAT-TM-7, COMMERCE/PAT-TM-8,...

  9. Multi-scale Functional and Molecular Photoacoustic Tomography

    PubMed Central

    Yao, Junjie; Xia, Jun; Wang, Lihong V.

    2015-01-01

    Photoacoustic tomography (PAT) combines rich optical absorption contrast with the high spatial resolution of ultrasound at depths in tissue. The high scalability of PAT has enabled anatomical imaging of biological structures ranging from organelles to organs. The inherent functional and molecular imaging capabilities of PAT have further allowed it to measure important physiological parameters and track critical cellular activities. Integration of PAT with other imaging technologies provides complementary capabilities and can potentially accelerate the clinical translation of PAT. PMID:25933617

  10. mRNA Expression of EgCHI1, EgCHI2, and EgCHI3 in Oil Palm Leaves (Elaeis guineesis Jacq.) after Treatment with Ganoderma boninense Pat. and Trichoderma harzianum Rifai

    PubMed Central

    Naher, Laila; Tan, Soon Guan; Ho, Chai Ling; Yusuf, Umi Kalsom; Ahmad, Siti Hazar; Abdullah, Faridah

    2012-01-01

    Background. Basal stem rot (BSR) disease caused by the fungus Ganoderma boninense is the most serious disease affecting the oil palm; this is because the disease escapes the early disease detection. The biocontrol agent Trichoderma harzianum can protect the disease only at the early stage of the disease. In the present study, the expression levels of three oil palm (Elaeis guineensis Jacq.) chitinases encoding EgCHI1, EgCHI2, and EgCHI3 at 2, 5, and 8 weeks inoculation were measured in oil palm leaves from plants treated with G. boninense or T. harzianum alone or both. Methods. The five-month-old oil palm seedlings were treated with Gano-wood blocks inoculum and trichomulch. Expression of EgCHI1, EgCHI2, and EgCHI3 in treated leaves tissue was determined by real-time PCR. Results. Oil palm chitinases were not strongly expressed in oil palm leaves of plants treated with G. boninense alone compared to other treatments. Throughout the 8-week experiment, expression of EgCHI1 increased more than 3-fold in leaves of plants treated with T. harzianum and G. boninense when compared to those of control and other treated plants. Conclusion. The data illustrated that chitinase cDNA expression varied depending on tissue and the type of treatment. PMID:22919345

  11. Planning the Beginning with the End in Mind: Evaluating Outreach and Enrollment Strategies, A Case Study. An Interview with Pat Redmond, Health Director, Philadelphia Citizens for Children and Youth, a NACA Member Organization. Issue Brief.

    ERIC Educational Resources Information Center

    Schmid, Margaret

    The State Children's Health Insurance Program (SCHIP) in 1997 signaled a major increase in time, energy, creativity, and money devoted to enrolling eligible children in health insurance programs. Child advocates and others have focused on developing outreach and enrollment strategies to bring the benefits of these new SCHIP programs to children…

  12. Pulse arrival time estimation from the impedance plethysmogram obtained with a handheld device.

    PubMed

    Gomez-Clapers, J; Casanella, R; Pallas-Areny, R

    2011-01-01

    This paper describes a novel method to estimate pulse arrival time (PAT) from the electrocardiogram (ECG) and the impedance plethysmogram (TPG) obtained by using a compact and easy-to-use handheld device with only four electrodes. A proof-of-concept has been carried out where PAT values obtained with the proposed device have been compared to PAT values measured between the ECG and the photoplethysmogram (PPG) during three experiments of paced respiration to induce controlled PAT changes. The results show that both methods yield equivalent PAT values in within ± 7 ms (95 % confidence interval), which is less than typical deviations reported for common PAT measurements. PMID:22254361

  13. Problemes theoriques et methodologiques dans l'etude des langues/dialectes en contact aux niveaux macrologique et micrologique = Theoretical and Methodological Issues in the Study of Languages/Dialects in Contact at Macro- and Micro-Logical Levels of Analysis. Proceedings of the International Conference DALE (University of London)/ICRB (Laval University, Quebec)/ICSBT (Vrije Universiteit te Brussel) (London, England, May 23-26, 1985).

    ERIC Educational Resources Information Center

    Blanc, Michel, Ed.; Hamers, Josiane F., Ed.

    Papers from an international conference on the interaction of languages and dialects in contact are presented in this volume. Papers include: "Quelques reflexions sur la variation linguistique"; "The Investigation of 'Language Continuum' and 'Diglossia': A Macrological Case Study and Theoretical Model"; "A Survey of Dialect Grammar in British…

  14. 76 FR 76412 - Change in Bank Control Notices; Acquisitions of Shares of a Bank or Bank Holding Company

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-07

    ..., Illinois 60690-1414: 1. Carl E. Stukenholtz, Perry, Iowa, individually, and acting in concert with James E... Memorial Drive, Kansas City, Missouri 64198-0001: 1. Dale Mitchell Ashlock, Gravois Mills, Missouri;...

  15. 78 FR 13315 - Bridger-Teton National Forest; Wyoming; Teton to Snake Fuels Management Project

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-27

    ... burned and unburned areas. Ground and aerial ignition techniques would adhere to site-specific burn plans... the agency with the ability to provide you with project updates. Dated: February 21, 2013. Dale...

  16. 3. VIEW NORTHEAST, SOUTH FRONT OF SOIL CONSERVATION SERVICE CLUSTER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW NORTHEAST, SOUTH FRONT OF SOIL CONSERVATION SERVICE CLUSTER (BUILDING 25) - U.S. Plant Introduction Station, Soil Conservation Service Cluster, 11601 Old Pond Road, Glenn Dale, Prince George's County, MD

  17. 2. VIEW SOUTHEAST, WEST FRONT OF BUILDINGS 25. 26; NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW SOUTHEAST, WEST FRONT OF BUILDINGS 25. 26; NORTH FRONT OF BUILDING 24: ROOF GABLE OF BUILDING 27 - U.S. Plant Introduction Station, Soil Conservation Service Cluster, 11601 Old Pond Road, Glenn Dale, Prince George's County, MD

  18. 1. VIEW EAST, WEST FRONT OF SOIL CONSERVATION SERVICE CLUSTER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW EAST, WEST FRONT OF SOIL CONSERVATION SERVICE CLUSTER (BUILDINGS 24, 25, 26) - U.S. Plant Introduction Station, Soil Conservation Service Cluster, 11601 Old Pond Road, Glenn Dale, Prince George's County, MD

  19. 33 CFR 211.81 - Reservoir areas.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., Tennessee. (q) Dale Hollow Reservoir Area, Tennessee. (r) Lake Cumberland, Kentucky. (s) Fort Supply... McClellan-Kerr Navigation Project in Oklahoma, to Former Owners Authority: Secs. 211.101 to...

  20. Open Rotor: New Option for Jet Engines

    NASA Video Gallery

    NASA's Dale Van Zante describes how the open rotor propulsion system will be tested in a wind tunnel at NASA's Glenn Research Center. Open rotor aircraft engines use high-speed propellers and are c...

  1. 51. LAUNCH CREW IN FRONT OF BARGE ON RAIL BUGGIES. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    51. LAUNCH CREW IN FRONT OF BARGE ON RAIL BUGGIES. (LEFT OT RIGHT: DALE WILBURN, GENE EVERLY, TIM DOEPELHEUER, JACK ROBERTS, JAMES REARICK, STEVE RAKUSKIN) - Hillman Barge & Construction Company, Paul Thomas Boulevard, Brownsville, Fayette County, PA

  2. 75 FR 40819 - Records Governing Off-the-Record Communications; Public Notice

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-14

    ... off-the-record communications. Order No. 607 (64 FR 51222, September 22, 1999) requires Commission... Byron Dale Simon). \\4\\ Record of telephone call with Matt Maraglio of New York State Division of...

  3. 1. Historic American Buildings Survey Roger Sturtevant, Photographer Mar. 13, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey Roger Sturtevant, Photographer Mar. 13, 1934 RESIDENCE STREET (Ukiah St.) - Ukiah Street (Houses), Ukiah Street between Howard & Lansing, Mendocino, Mendocino County, CA

  4. 2. Historic American Buildings Survey E. H. Pickering, Photographer July ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey E. H. Pickering, Photographer July 1936 FORMERLY COMMERCIAL & FARMERS BANK. MAXIMILLAN GODFROY ARCHITECT - Howard & Redwood Streets (Commercial Building), Baltimore, Independent City, MD

  5. 75 FR 51837 - Iowa; Amendment No. 2 to Notice of a Major Disaster Declaration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-23

    ... Individual Assistance. Black Hawk, Cherokee, Clayton, Decatur, Delaware, Dubuque, Fayette, Franklin, Howard..., Webster, and Wright Counties for Individual Assistance (already designated for Public Assistance)....

  6. 78 FR 51203 - Iowa; Major Disaster and Related Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-20

    ... determinations. DATES: Effective Date: July 31, 2013. FOR FURTHER INFORMATION CONTACT: Dean Webster, Office of...: Allamakee, Benton, Buchanan, Butler, Cedar, Clayton, Delaware, Howard, Jones, and Winneshiek Counties...

  7. 76 FR 60799 - Senior Executive Services (SES) Performance Review Board: Update

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-30

    ... International Development, Office of Inspector General: Michael G. Carroll, Deputy Inspector General. Howard I... Management. Lisa S. Goldfluss, Legal Counsel. Alvin A. Brown, Assistant Inspector General,...

  8. 40 CFR 81.28 - Metropolitan Baltimore Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF AREAS FOR AIR QUALITY PLANNING PURPOSES Designation of... Arundel County, Baltimore City, Baltimore County, Carroll County, Harford County, Howard County....

  9. 40 CFR 81.28 - Metropolitan Baltimore Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF AREAS FOR AIR QUALITY PLANNING PURPOSES Designation of... Arundel County, Baltimore City, Baltimore County, Carroll County, Harford County, Howard County....

  10. 40 CFR 81.28 - Metropolitan Baltimore Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF AREAS FOR AIR QUALITY PLANNING PURPOSES Designation of... Arundel County, Baltimore City, Baltimore County, Carroll County, Harford County, Howard County....

  11. 40 CFR 81.28 - Metropolitan Baltimore Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF AREAS FOR AIR QUALITY PLANNING PURPOSES Designation of... Arundel County, Baltimore City, Baltimore County, Carroll County, Harford County, Howard County....

  12. 76 FR 44025 - Missouri; Emergency and Related Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-22

    ..., Chariton, Clark, Clay, Cole, Cooper, Franklin, Gasconade, Holt, Howard, Jackson, Lafayette, Lewis, Moniteau, Montgomery, Osage, Platte, Ray, Saline, St. Charles, St. Louis, and Warren Counties, and the Independent...

  13. Transgenic cry1C(⁎) gene rough rice line T1C-19 does not change the host preferences of the non-target stored product pest, Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae), and its parasitoid wasp, Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae).

    PubMed

    Sun, Xiao; Yan, Miao-Jun; Zhang, Aijun; Wang, Man-Qun

    2015-10-01

    Rough rice grains are often stored for extended periods before they are used or consumed. However, during storage, the rough rice is vulnerable to insect infestation, resulting in significant economic loss. Previous studies have shown that volatiles cues, physical characteristics, and taste chemicals on the grains could be the important key behavior factors for storage insect pests to locate the hosts and select oviposition sites. It is also well known that the transgenic Bt rough rice line T1C-19, which expresses a cry1C(⁎) gene has a high resistance to Lepidoptera pests. However, there were no evidences to show the consequences of host preference for non-target insect pests after growing Bt transgenic rice. In this study, the potential key factors of Bt rough rice were investigated for their impacts on the behaviors of non-target pest lesser grain borer Rhyzopertha dominica, the main weevil pest of grain and its parasitic wasps Anisopteromalus calandrae, the natural enemy of the beetle. Both electronic nose and electronic tongue analyses showed that the parameters of Bt rough rice were analogous to those of the non-Bt rough rice. The volatile profiles of Bt and non-Bt rough rice examined by gas chromatographic mass spectrometry (GC-MS) were similar. For most volatile compounds, there were no significantly quantitative differences in compound quantities between Bt and non-Bt rough rice. The densities of sclereids and trichomes on the rough rice husk surface were statistically equal in Bt and non-Bt rough rice. The non-target pest, R. dominica, and its parasitoid wasp, A. calandrae, were attracted to both rough rice and could not distinguish the transgenic T1C-19 from the isogenic rough rice. These results demonstrated that Bt rough rice has no negative impacts on the host preference behaviors of non-target stored product pest R. dominica and its parasitoid A. calandrae. PMID:26150137

  14. Biofuels: thinking clearly about the issues.

    PubMed

    Dale, Bruce

    2008-06-11

    Dr. Bruce Dale is the recipient of the 2007 Sterling B. Hendricks Memorial Lectureship Award. This perspective is based on a lecture given by Dr. Dale at the Life Sciences and Society Symposium at the University of Missouri on March 14, 2007, and the Award Address he presented for the Sterling B. Hendricks Memorial Lectureship Award at the 234th National Meeting of the American Chemical Society, in Boston, MA, on August 20, 2007. PMID:18422330

  15. 76 FR 66718 - Clean Air Act Advisory Committee (CAAAC): Notice of Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-27

    ..., please contact Pat Childers, Office of Air and Radiation, U.S. EPA (202) 564-1082, FAX (202) 564-1352 or...-1082 or childers.pat@epa.gov . To request accommodation of a disability, please contact Mr....

  16. Tutorial on photoacoustic tomography

    NASA Astrophysics Data System (ADS)

    Zhou, Yong; Yao, Junjie; Wang, Lihong V.

    2016-06-01

    Photoacoustic tomography (PAT) has become one of the fastest growing fields in biomedical optics. Unlike pure optical imaging, such as confocal microscopy and two-photon microscopy, PAT employs acoustic detection to image optical absorption contrast with high-resolution deep into scattering tissue. So far, PAT has been widely used for multiscale anatomical, functional, and molecular imaging of biological tissues. We focus on PAT's basic principles, major implementations, imaging contrasts, and recent applications.

  17. 78 FR 16837 - Privacy Act of 1974; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-19

    .../ PAT-TM-20 Customer Call Center, Assistance and Satisfaction Survey Records.'' This action is being... methods: Email: Richard.Fernandez@uspto.gov . Include ``Privacy Act PAT-TM-20 comment'' in the subject... Privacy Act system of records notice, ``COMMERCE/PAT- TM-20 Customer Call Center, Assistance...

  18. 78 FR 19463 - Privacy Act of 1974; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-01

    .../ PAT-TM-13 Petitioners for License to File for Foreign Patents.'' This action is being taken to update...@uspto.gov . Include ``Privacy Act PAT- TM-13 comment'' in the subject line of the message. Fax: (571... Privacy Act system of records notice, ``COMMERCE/PAT- TM-13 Petitioners for License to File for...

  19. 78 FR 19246 - Privacy Act of 1974; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-29

    .../ PAT-TM-8 Patent Application Secrecy Order Files.'' This action is being taken to update the Privacy...@uspto.gov . Include ``Privacy Act PAT- TM-8 comment'' in the subject line of the message. Fax: (571) 273... system of records notice, ``COMMERCE/PAT- TM-8 Patent Application Secrecy Order Files,'' is published...

  20. 78 FR 19243 - Privacy Act of 1974; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-29

    .../ PAT-TM-7 Patent Application Files.'' This action is being taken to update the Privacy Act notice. We... Act PAT- TM-7 comment'' in the subject line of the message. Fax: (571) 273-7728, marked to the.... The amended Privacy Act system of records notice, ``COMMERCE/PAT- TM-7 Patent Application Files,''...

  1. 78 FR 19247 - Privacy Act of 1974; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-29

    .../ PAT-TM-6 Parties Involved in Patent Interference Proceedings.'' This action is being taken to update...@uspto.gov . Include ``Privacy Act PAT-TM- 6 comment'' in the subject line of the message. Fax: (571) 273... related to the USPTO. The amended Privacy Act system of records notice, ``COMMERCE/PAT- TM-6...

  2. 78 FR 19244 - Privacy Act of 1974; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-29

    .../ PAT-TM-9 Patent Assignment Records.'' This action is being taken to update the Privacy Act notice. We... ``Privacy Act PAT- TM-9 comment'' in the subject line of the message. Fax: (571) 273-0140, marked to the... notice, ``COMMERCE/PAT- TM-9 Patent Assignment Records,'' is published in its entirety below....

  3. 78 FR 19462 - Privacy Act of 1974; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-01

    ... Trademark Office (USPTO) is amending the system of records currently listed under ``COMMERCE/ PAT-TM-11... PAT- TM-11 comment'' in the subject line of the message. Fax: (571) 273-0373, marked to the attention... records notice, ``COMMERCE/PAT- TM-11 Patent Examiner Testimony Files,'' is published in its...

  4. 76 FR 77979 - Sunset of the Patent Application Backlog Reduction Stimulus Plan and a Limited Extension of the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-15

    ... Application Backlog Reduction Stimulus Plan, 74 FR 62285 (Nov. 27, 2009), 1349 Off. Gaz. Pat. Office 304 (Dec... Reduction Stimulus Plan, 74 FR at 62287, 1349 Off. Gaz. Pat. Office at 306. The USPTO extended the plan for... Stimulus Plan, 75 FR 5041 (Feb. 1, 2010), 1351 Off. Gaz. Pat. Office 202 (Feb. 23, 2010). Subsequently,...

  5. 75 FR 71072 - Extension of the Patent Application Backlog Reduction Stimulus Plan

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-22

    ... Stimulus Plan, 74 FR 62285 (Nov. 27, 2009), 1349 Off. Gaz. Pat. Off. 304 (Dec. 22, 2009) (notice). The... Reduction Stimulus Plan, 74 FR at 62287, 1349 Off. Gaz. Pat. Off. at 306. The USPTO extended the plan for an... Plan, 75 FR 5041 (February 1, 2010), 1351 Off. Gaz. Pat. Off. 202 (February 23, 2010)....

  6. Partnership for a Drug-Free America: Partnership Attitude Tracking Study. Teens: Ethnic and Racial Trends, Spring 2002.

    ERIC Educational Resources Information Center

    Delaney, Barbara

    The annual Partnership Attitude Tracking Study (PATS) tracks consumers' attitudes about illegal drugs. PATS consists of two nationally projectable samples: a teen sample for students in grades 7-12 and a parent sample. The 2002 PATS, conducted in homes and schools, collected data using self-report surveys. Results indicate that after a decade of…

  7. Watch Walking to Gauge Health After Heart Surgery

    MedlinePlus

    ... Dr. Howard Levite, who directs cardiology at Staten Island University Hospital, in Staten Island, N.Y. "Surprisingly, it is the obese, diabetic ... Y.; Howard Levite, M.D., director, cardiology, Staten Island University Hospital, Staten Island, N.Y.; JAMA Cardiology , ...

  8. Grants for Science Education 1997. Including Grants for Research Resources in the United States and for Biomedical Scientists Abroad.

    ERIC Educational Resources Information Center

    Howard Hughes Medical Inst., Chevy Chase, MD. Office of Grants and Special Programs.

    The data presented in this document provide information about those individuals and organizations that received funding from the Howard Hughes Medical Institute in 1997. Following a description of the Howard Hughes Medical Institute programs, details on the funding of graduate science education, undergraduate biological sciences education,…

  9. The Role of Home-Visiting Programs in Preventing Child Abuse and Neglect

    ERIC Educational Resources Information Center

    Howard, Kimberly S.; Brooks-Gunn, Jeanne

    2009-01-01

    Kimberly Howard and Jeanne Brooks-Gunn examine home visiting, an increasingly popular method for delivering services for families, as a strategy for preventing child abuse and neglect. They focus on early interventions because infants are at greater risk for child abuse and neglect than are older children. In their article, Howard and Brooks-Gunn…

  10. 77 FR 56138 - World Trade Center Health Program; Addition of Certain Types of Cancer to the List of WTC-Related...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-12

    ... proposed rulemaking (77 FR 35574) proposing to add certain cancers to the List of WTC-Related Health... published on June 13, 2012 (77 FR 35574). \\5\\ Howard J . October 5, 2011 Letter from John Howard, MD..., 2012. \\8\\ WHO International Agency for Research on Cancer (IARC). http://monographs.iarc.fr/ ....

  11. Travel and Correspondence

    ERIC Educational Resources Information Center

    Shauck, Barry

    2004-01-01

    Professional development through the advanced planning of themed exhibitions provides art instructors in Howard County the impetus for many art problems. This art problem, centered on the art and writing contained in the storybooks of Holly Hobbie. In late summer, the art teaching staff of the Howard County Public School System gathered at the…

  12. The Art of Math

    ERIC Educational Resources Information Center

    Kim, Una; Stabley, Angela

    2010-01-01

    In the movie "A Beautiful Mind" there is a scene where future Nobel prize winner John Nash is captivated by a fellow student's tie. When asked about his behavior Nash quips, "There has to be a mathematical explanation for how bad that tie is." (Grazer & Howard, 2000) In this light moment, director Ron Howard attempts to show us that the mind of a…

  13. Aussie Battler, or Worldly Opportunist?

    ERIC Educational Resources Information Center

    Walter, James

    2004-01-01

    In this article, the author comments on Judith Brett's article (see EJ848172). Brett dissects the differences between the Howard world-view and that of the intelligentsia, using Robert Merton's dichotomy between "locals" and "cosmopolitans" as her means. On this account, Howard's preoccupation with national sovereignty--and hence with border…

  14. What Helps Us Learn?

    ERIC Educational Resources Information Center

    Educational Leadership, 2010

    2010-01-01

    This article presents comments of high school students at the Howard Gardner School in Alexandria, Virginia, who were asked, What should teachers know about students to help them learn? Twelve high school students from the Howard Gardner School in Alexandria, Virginia, describe how their best teachers get to know them and thus were more able to…

  15. A World without Walls. Dimension: Languages 71. Proceedings of the Southern Conference on Language Teaching (7th, Atlanta, Georgia, October 1971).

    ERIC Educational Resources Information Center

    Wilkins, George W., Jr., Ed.

    Presentations on aspects of foreign language instruction include: "A World Without Walls" (Lorraine A. Strasheim); "Practical Aspects of Individualizing Foreign Language Instruction" (Howard B. Altman); "Teaching Foreign Language Through Literature" (Jeanne Varney Pleasants); "Individualization of Foreign Language Instruction" (Howard B. Altman);…

  16. Changing Views of Culture and Behavior: An Interview with Robert Serpell

    ERIC Educational Resources Information Center

    Lockett, Charles T.

    2003-01-01

    Charles Lockett is an Assistant Professor in the School of Psychology at James Madison University, where he teaches developmental psychology as well as advanced topic courses in cultural psychology and the psychology of race and racism. A graduate of Howard University, Lockett credits Howard's Preparing Future Faculty Fellowship Program for his…

  17. Leadership under the Golden Rule

    ERIC Educational Resources Information Center

    Hebel, Sara

    2009-01-01

    The author interviews Muriel A. Howard, president of the State University of New York College at Buffalo, who is set to become the first black leader of any of the "big six" higher-education associations in Washington. She was named last week as the new president of the American Association of State Colleges and Universities. Ms. Howard, 60, is…

  18. A Practical Guide to Photoacoustic Tomography in the Life Sciences

    PubMed Central

    Wang, Lihong V.; Yao, Junjie

    2016-01-01

    The life sciences can benefit greatly from imaging technologies that connect microscopic discoveries with macroscopic observations. Photoacoustic tomography (PAT), a highly sensitive modality for imaging rich optical absorption contrast over a wide range of spatial scales at high speed, is uniquely positioned for this need. In PAT, endogenous contrast reveals tissue’s anatomical, functional, metabolic, and histologic properties, and exogenous contrast provides molecular and cellular specificity. The spatial scale of PAT covers organelles, cells, tissues, organs, and small-animal organisms. Consequently, PAT is complementary to other imaging modalities in contrast mechanism, penetration, spatial resolution, and temporal resolution. We review the fundamentals of PAT and provide practical guidelines to the broad life science community for matching PAT systems with research needs. We also summarize the most promising biomedical applications of PAT, discuss related challenges, and envision its potential to lead to further breakthroughs. PMID:27467726

  19. A Golgi and tonoplast localized S-acyl transferase is involved in cell expansion, cell division, vascular patterning and fertility in Arabidopsis

    PubMed Central

    Qi, Baoxiu; Doughty, James; Hooley, Richard

    2013-01-01

    S-acylation of eukaryotic proteins is the reversible attachment of palmitic or stearic acid to cysteine residues, catalysed by protein S-acyl transferases that share an Asp-His-His-Cys (DHHC) motif. Previous evidence suggests that in Arabidopsis S-acylation is involved in the control of cell size, polarity and the growth of pollen tubes and root hairs. Using a combination of yeast genetics, biochemistry, cell biology and loss of function genetics the roles of a member of the protein S-acyl transferase PAT family, AtPAT10 (At3g51390), have been explored. In keeping with its role as a PAT, AtPAT10 auto-S-acylates, and partially complements the yeast akr1 PAT mutant, and this requires Cys192 of the DHHC motif. In Arabidopsis AtPAT10 is localized in the Golgi stack, trans-Golgi network/early endosome and tonoplast. Loss-of-function mutants have a pleiotropic phenotype involving cell expansion and division, vascular patterning, and fertility that is rescued by wild-type AtPAT10 but not by catalytically inactive AtPAT10C192A. This supports the hypothesis that AtPAT10 is functionally independent of the other Arabidopsis PATs. Our findings demonstrate a growing importance of protein S-acylation in plants, and reveal a Golgi and tonoplast located S-acylation mechanism that affects a range of events during growth and development in Arabidopsis. PMID:23795888

  20. Precocious leaf senescence by functional loss of PROTEIN S-ACYL TRANSFERASE14 involves the NPR1-dependent salicylic acid signaling

    PubMed Central

    Zhao, Xin-Ying; Wang, Jia-Gang; Song, Shi-Jian; Wang, Qun; Kang, Hui; Zhang, Yan; Li, Sha

    2016-01-01

    We report here that Arabidopsis PROTEIN S-ACYL TRANSFERASE14 (PAT14), through its palmitate transferase activity, acts at the vacuolar trafficking route to repress salicylic acid (SA) signaling, thus mediating age-dependent but not carbon starvation-induced leaf senescence. Functional loss of PAT14 resulted in precocious leaf senescence and its transcriptomic analysis revealed that senescence was dependent on salicylic acid. Overexpressing PAT14 suppressed the expression of SA responsive genes. Introducing the SA deficient mutants, npr1-5 and NahG, but not other hormonal mutants, completely suppressed the precocious leaf senescence of PAT14 loss-of-function, further supporting the epistatic relation between PAT14 and the SA pathway. By confocal fluorescence microscopy, we showed that PAT14 is localized at the Golgi, the trans-Golg network/early endosome, and prevacuolar compartments, indicating its roles through vacuolar trafficking. By reporter analysis and real time PCRs, we showed that the expression PAT14, unlike most of the senescence associated genes, is not developmentally regulated, suggesting post-transcriptional regulatory mechanisms on its functionality. We further showed that the maize and wheat homologs of PAT14 fully rescued the precocious leaf senescence of pat14-2, demonstrating that the role of PAT14 in suppressing SA signaling during age-dependent leaf senescence is evolutionarily conserved between dicots and monocots. PMID:26842807

  1. Patulin is a cultivar-dependent aggressiveness factor favouring the colonization of apples by Penicillium expansum.

    PubMed

    Snini, Selma P; Tannous, Joanna; Heuillard, Pauline; Bailly, Sylviane; Lippi, Yannick; Zehraoui, Enric; Barreau, Christian; Oswald, Isabelle P; Puel, Olivier

    2016-08-01

    The blue mould decay of apples is caused by Penicillium expansum and is associated with contamination by patulin, a worldwide regulated mycotoxin. Recently, a cluster of 15 genes (patA-patO) involved in patulin biosynthesis was identified in P. expansum. blast analysis revealed that patL encodes a Cys6 zinc finger regulatory factor. The deletion of patL caused a drastic decrease in the expression of all pat genes, leading to an absence of patulin production. Pathogenicity studies performed on 13 apple varieties indicated that the PeΔpatL strain could still infect apples, but the intensity of symptoms was weaker compared with the wild-type strain. A lower growth rate was observed in the PeΔpatL strain when this strain was grown on nine of the 13 apple varieties tested. In the complemented PeΔpatL:patL strain, the ability to grow normally in apple and the production of patulin were restored. Our results clearly demonstrate that patulin is not indispensable in the initiation of the disease, but acts as a cultivar-dependent aggressiveness factor for P. expansum. This conclusion was strengthened by the fact that the addition of patulin to apple infected by the PeΔpatL mutant restored the normal fungal colonization in apple. PMID:26582186

  2. Proton-assisted amino acid transporters are conserved regulators of proliferation and amino acid-dependent mTORC1 activation

    PubMed Central

    Heublein, S; Kazi, S; Ögmundsdóttir, M H; Attwood, E V; Kala, S; Boyd, C A R; Wilson, C; Goberdhan, D C I

    2011-01-01

    The PI3-kinase (PI3K)/Akt and downstream mammalian target of rapamycin complex 1 (mTORC1) signalling cascades promote normal growth and are frequently hyperactivated in tumour cells. mTORC1 is also regulated by local nutrients, particularly amino acids, but the mechanisms involved are poorly understood. Unexpectedly, members of the proton-assisted amino acid transporter (PAT or SLC36) family emerged from in vivo genetic screens in Drosophila as transporters with uniquely potent effects on mTORC1-mediated growth. Here we show the two human PATs that are widely expressed in normal tissues and cancer cell lines, PAT1 and PAT4, behave similarly to fly PATs when expressed in Drosophila. siRNA knockdown reveals that these molecules are required for activation of mTORC1 targets and for proliferation in human MCF-7 breast cancer and HEK-293 embryonic kidney cell lines. Furthermore, activation of mTORC1 in starved HEK-293 cells stimulated by amino acids requires PAT1 and PAT4, and is elevated in PAT1-overexpressing cells. Importantly, in HEK-293 cells, PAT1 is highly concentrated in intracellular compartments, including endosomes, where mTOR shuttles upon amino acid stimulation. Our data are therefore consistent with a model in which PATs modulate mTORC1's activity not by transporting amino acids into the cell, but by modulating the intracellular response to amino acids. PMID:20498635

  3. Relationships between the ABC-Exporter HetC and Peptides that Regulate the Spatiotemporal Pattern of Heterocyst Distribution in Anabaena

    PubMed Central

    Corrales-Guerrero, Laura; Flores, Enrique; Herrero, Antonia

    2014-01-01

    In the model cyanobacterium Anabaena sp. PCC 7120, cells called heterocysts that are specialized in the fixation of atmospheric nitrogen differentiate from vegetative cells of the filament in the absence of combined nitrogen. Heterocysts follow a specific distribution pattern along the filament, and a number of regulators have been identified that influence the heterocyst pattern. PatS and HetN, expressed in the differentiating cells, inhibit the differentiation of neighboring cells. At least PatS appears to be processed and transferred from cell to cell. HetC is similar to ABC exporters and is required for differentiation. We present an epistasis analysis of these regulatory genes and of genes, hetP and asr2819, successively downstream from hetC, and we have studied the localization of HetC and HetP by use of GFP fusions. Inactivation of patS, but not of hetN, allowed differentiation to proceed in a hetC background, whereas inactivation of hetC in patS or patS hetN backgrounds decreased the frequency of contiguous proheterocysts. A HetC-GFP protein is localized to the heterocysts and especially near their cell poles, and a putative HetC peptidase domain was required for heterocyst differentiation but not for HetC-GFP localization. hetP is also required for heterocyst differentiation. A HetP-GFP protein localized mostly near the heterocyst poles. ORF asr2819, which we denote patC, encodes an 84-residue peptide and is induced upon nitrogen step-down. Inactivation of patC led to a late spreading of the heterocyst pattern. Whereas HetC and HetP appear to have linked functions that allow heterocyst differentiation to progress, PatC may have a role in selecting sites of differentiation, suggesting that these closely positioned genes may be functionally related. PMID:25121608

  4. Relationships between the ABC-exporter HetC and peptides that regulate the spatiotemporal pattern of heterocyst distribution in Anabaena.

    PubMed

    Corrales-Guerrero, Laura; Flores, Enrique; Herrero, Antonia

    2014-01-01

    In the model cyanobacterium Anabaena sp. PCC 7120, cells called heterocysts that are specialized in the fixation of atmospheric nitrogen differentiate from vegetative cells of the filament in the absence of combined nitrogen. Heterocysts follow a specific distribution pattern along the filament, and a number of regulators have been identified that influence the heterocyst pattern. PatS and HetN, expressed in the differentiating cells, inhibit the differentiation of neighboring cells. At least PatS appears to be processed and transferred from cell to cell. HetC is similar to ABC exporters and is required for differentiation. We present an epistasis analysis of these regulatory genes and of genes, hetP and asr2819, successively downstream from hetC, and we have studied the localization of HetC and HetP by use of GFP fusions. Inactivation of patS, but not of hetN, allowed differentiation to proceed in a hetC background, whereas inactivation of hetC in patS or patS hetN backgrounds decreased the frequency of contiguous proheterocysts. A HetC-GFP protein is localized to the heterocysts and especially near their cell poles, and a putative HetC peptidase domain was required for heterocyst differentiation but not for HetC-GFP localization. hetP is also required for heterocyst differentiation. A HetP-GFP protein localized mostly near the heterocyst poles. ORF asr2819, which we denote patC, encodes an 84-residue peptide and is induced upon nitrogen step-down. Inactivation of patC led to a late spreading of the heterocyst pattern. Whereas HetC and HetP appear to have linked functions that allow heterocyst differentiation to progress, PatC may have a role in selecting sites of differentiation, suggesting that these closely positioned genes may be functionally related. PMID:25121608

  5. Delivery of paclitaxel from cobalt–chromium alloy surfaces without polymeric carriers

    PubMed Central

    Mani, Gopinath; Macias, Celia E.; Feldman, Marc D.; Marton, Denes; Oh, Sunho; Agrawal, C. Mauli

    2014-01-01

    Polymer-based carriers are commonly used to deliver drugs from stents. However, adverse responses to polymer coatings have raised serious concerns. This research is focused on delivering drugs from stents without using polymers or any carriers. Paclitaxel (PAT), an anti-restenotic drug, has strong adhesion towards a variety of material surfaces. In this study, we have utilized such natural adhesion property of PAT to attach these molecules directly to cobalt–chromium (Co–Cr) alloy, an ultra-thin stent strut material. Four different groups of drug coated specimens were prepared by directly adding PAT to Co–Cr alloy surfaces: Group-A (PAT coated, unheated, and ethanol cleaned); Group-B (PAT coated, heat treated, and ethanol cleaned); Group-C (PAT coated, unheated, and not ethanol cleaned); and Group-D (PAT coated, heat treated and not ethanol cleaned). In vitro drug release of these specimens was investigated using high performance liquid chromatography. Groups A and B showed sustained PAT release for up to 56 days. A simple ethanol cleaning procedure after PAT deposition can remove the loosely bound drug crystals from the alloy surfaces and thereby allowing the remaining strongly bound drug molecules to be released at a sustained rate. The heat treatment after PAT coating further improved the stability of PAT on Co–Cr alloy and allowed the drug to be delivered at a much slower rate, especially during the initial 7 days. The specimens which were not cleaned in ethanol, Groups C and D, showed burst release. PAT coated Co–Cr alloy specimens were thoroughly characterized using scanning electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy. These techniques were collectively useful in studying the morphology, distribution, and attachment of PAT molecules on Co–Cr alloy surfaces. Thus, this study suggests the potential for delivering paclitaxel from Co–Cr alloy surfaces without using any carriers. PMID:20398928

  6. New evidence for the age of the Quantico Formation of Virginia

    NASA Astrophysics Data System (ADS)

    Pavlides, Louis; Pojeta, John, Jr.; Gordon, Mackenzie, Jr.; Parsley, R. L.; Bobyarchick, A. R.

    1980-06-01

    A new fossil locality within the Quantico Formation of the Virginia Piedmont in Dale City, Virginia, about 40 km south of Washington, D.C., has yielded crinoids and a cephalopod that re-establishes an age younger than Cambrian for this formation. On the basis of regional considerations and the work of investigators in the early 1900s, an Ordovician age for the Quantico seems likely. Also, the Dale City pluton is not intrusive into the Quantico but is unconformable beneath it. Hence, the 560-m.y. zircon age for the Dale City pluton is no longer inconsistent with the temporal and stratigraphic relationships established for the stratigraphic units in the area. *Present address: State University of New York at Albany, Albany, New York 12222

  7. Discrete dynamical classes for galaxy discs and the implication of a second generation of Tully-Fisher methods

    NASA Astrophysics Data System (ADS)

    Roscoe, D. F.

    2002-04-01

    In Roscoe (\\cite{RoscoeA}), it was described how the modelling of a small sample of optical rotation curves (ORCs) given by Rubin et al. (\\cite{Rubin}) with the power-law Vrot=ARα, where where the parameters (A,alpha ) vary between galaxies, raised the hypothesis that the parameter A (considered in the form ln A) had a preference for certain discrete values. This specific hypothesis was tested in that paper against a sample of 900 spiral galaxy rotation curves measured by Mathewson et al. (\\cite{Mathewson1992}), but folded by Persic & Salucci (\\cite{Persic1995}), and was confirmed on this large sample with a conservatively estimated upper bound probability of 10-7 against it being a chance effect. In this paper, we begin by reviewing the earlier work, and then describe the analyses of three additional samples; the first of these, of 1200+ Southern sky ORCs, was published by Mathewson & Ford (\\cite{Mathewson1996}), the second, of 497 Northern sky ORCs, is a composite sample provided by kind permission of Giovanelli & Haynes published in the sequence of papers Dale et al. (\\cite{Dale1997}, \\cite{Dale1998}, \\cite{Dale1999}) and Dale & Uson (\\cite{Dale2000}), whilst the third, of 305 Northern sky ORCs, was published by Courteau (\\cite{Courteau}). These analyses provide overwhelmingly compelling confirmation of what was already a powerful result. Apart from other considerations, the results lead directly to what can be described as a ``second generation of Tully-Fisher methods''. We give a brief discussion of the further implications of the result.

  8. Tissue oxidative stress induced by patulin and protective effect of crocin.

    PubMed

    Boussabbeh, Manel; Ben Salem, Intidhar; Belguesmi, Faicel; Bacha, Hassen; Abid-Essefi, Salwa

    2016-03-01

    Patulin (PAT) is a secondary toxic metabolite produced principally by Penicillium expansum. This mycotoxin is known to be teratogenic, mutagenic, immunotoxic and neurotoxic, and it has been shown to cause damage in several organs in laboratory animals. This study focuses on the prevention of experimental murine PAT-induced nephrotoxicity and hepatotoxicity. We investigate the ability of a natural product, crocin (CRO), to counteract the toxic effects of PAT. Pre-treatment of mice with CRO prevented PAT-induced oxidative damage in both liver and kidney. CRO reduced lipid peroxidation, protein oxidation and restored redox status by regulating the endogenous antioxidant enzymatic system. These data corroborate and extend findings in PAT-induced nephrotoxicity and hepatotoxicity, and further suggest that preventive effect of CRO towards other forms of PAT toxicity, including neurotoxicity, may be warranted. PMID:26584762

  9. 4-D Photoacoustic Tomography

    PubMed Central

    Xiang, Liangzhong; Wang, Bo; Ji, Lijun; Jiang, Huabei

    2013-01-01

    Photoacoustic tomography (PAT) offers three-dimensional (3D) structural and functional imaging of living biological tissue with label-free, optical absorption contrast. These attributes lend PAT imaging to a wide variety of applications in clinical medicine and preclinical research. Despite advances in live animal imaging with PAT, there is still a need for 3D imaging at centimeter depths in real-time. We report the development of four dimensional (4D) PAT, which integrates time resolutions with 3D spatial resolution, obtained using spherical arrays of ultrasonic detectors. The 4D PAT technique generates motion pictures of imaged tissue, enabling real time tracking of dynamic physiological and pathological processes at hundred micrometer-millisecond resolutions. The 4D PAT technique is used here to image needle-based drug delivery and pharmacokinetics. We also use this technique to monitor 1) fast hemodynamic changes during inter-ictal epileptic seizures and 2) temperature variations during tumor thermal therapy. PMID:23346370

  10. Contrast-enhanced photoacoustic tomography of human joints

    NASA Astrophysics Data System (ADS)

    Tian, Chao; Keswani, Rahul K.; Gandikota, Girish; Rosania, Gus R.; Wang, Xueding

    2016-03-01

    Photoacoustic tomography (PAT) provides a unique tool to diagnose inflammatory arthritis. However, the specificity and sensitivity of PAT based on endogenous contrasts is limited. The development of contrast enhanced PAT imaging modalities in combination with small molecule contrast agents could lead to improvements in diagnosis and treatment of joint disease. Accordingly, we adapted and tested a PAT clinical imaging system for imaging the human joints, in combination with a novel PAT contrast agent derived from an FDA-approved small molecule drug. Imaging results based on a photoacoustic and ultrasound (PA/US) dual-modality system revealed that this contrast-enhanced PAT imaging system may offer additional information beyond single-modality PA or US imaging system, for the imaging, diagnosis and assessment of inflammatory arthritis.

  11. 4-D Photoacoustic Tomography

    NASA Astrophysics Data System (ADS)

    Xiang, Liangzhong; Wang, Bo; Ji, Lijun; Jiang, Huabei

    2013-01-01

    Photoacoustic tomography (PAT) offers three-dimensional (3D) structural and functional imaging of living biological tissue with label-free, optical absorption contrast. These attributes lend PAT imaging to a wide variety of applications in clinical medicine and preclinical research. Despite advances in live animal imaging with PAT, there is still a need for 3D imaging at centimeter depths in real-time. We report the development of four dimensional (4D) PAT, which integrates time resolutions with 3D spatial resolution, obtained using spherical arrays of ultrasonic detectors. The 4D PAT technique generates motion pictures of imaged tissue, enabling real time tracking of dynamic physiological and pathological processes at hundred micrometer-millisecond resolutions. The 4D PAT technique is used here to image needle-based drug delivery and pharmacokinetics. We also use this technique to monitor 1) fast hemodynamic changes during inter-ictal epileptic seizures and 2) temperature variations during tumor thermal therapy.

  12. Tutorial on photoacoustic tomography.

    PubMed

    Zhou, Yong; Yao, Junjie; Wang, Lihong V

    2016-06-01

    Photoacoustic tomography (PAT) has become one of the fastest growing fields in biomedical optics. Unlike pure optical imaging, such as confocal microscopy and two-photon microscopy, PAT employs acoustic detection to image optical absorption contrast with high-resolution deep into scattering tissue. So far, PAT has been widely used for multiscale anatomical, functional, and molecular imaging of biological tissues. We focus on PAT’s basic principles, major implementations, imaging contrasts, and recent applications. PMID:27086868

  13. Then & Now: Medical Research Pays Off for All Americans

    MedlinePlus

    ... Then & Now Medical Research Pays Off for All Americans Past Issues / Fall 2007 Table of Contents For ... two conditions which afflict tens of millions of Americans of all ages. William Howard Taft—Then & Now ...

  14. A Psychological View of Spirituality and Leadership.

    ERIC Educational Resources Information Center

    Solomon, Jeffrey; Hunter, Jeremy

    2002-01-01

    Using Howard Gardner's concept of existential intelligence along with others such as Jerome Bruner, explores the psychology of spirituality and leadership. Describes how famous film director uses meditation in his work. Draws implications for educational leadership. (PKP)

  15. 78 FR 54651 - Maximum Per Diem Rates for the Continental United States (CONUS)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-05

    ... identified two new non-standard areas (NSAs): Big Spring, TX (Howard County); and Pearsall, TX (Frio, La... Internet at www.gsa.gov/perdiem . This process, implemented in 2003, ensures more timely changes in...

  16. 10. INTERIOR DETAIL, FIRST STORY, SHOWING RELAY RACKS Baltimore ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. INTERIOR DETAIL, FIRST STORY, SHOWING RELAY RACKS - Baltimore & Potomac Interlocking Tower, Adjacent to AMTRAK railroad tracks in block bounded by Howard Street, Jones Falls Expressway, Maryland Avenue & Falls Road, Baltimore, Independent City, MD

  17. 5. View of north elevation, including saw dust collector and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. View of north elevation, including saw dust collector and brick addition, looking south east. - General Dynamics Corporation Shipyard, Joiner & Sheet Metal Shops, 97 East Howard Street, Quincy, Norfolk County, MA

  18. Parenting Your Adopted Teenager

    MedlinePlus

    ... https: / / www. childwelfare. gov/ pubs/ f- openadopt/ .) The Internet and the explosion of social media sites (e. ... 4 Howard, J. (2012). Untangling the web: The Internet’s transformative impact on adoption . New York, NY: Evan ...

  19. Sequencing Complex Genomic Regions

    SciTech Connect

    Eichler, Evan

    2009-05-28

    Evan Eichler, Howard Hughes Medical Investigator at the University of Washington, gives the May 28, 2009 keynote speech at the "Sequencing, Finishing, Analysis in the Future" meeting in Santa Fe, NM. Part 1 of 2

  20. Sequencing Complex Genomic Regions

    SciTech Connect

    Eichler, Evan

    2009-05-28

    Evan Eichler, Howard Hughes Medical Investigator at the University of Washington, gives the May 28, 2009 keynote speech at the "Sequencing, Finishing, Analysis in the Future" meeting in Santa Fe, NM. Part 2 of 2

  1. It Bears Repeating: Understanding the Work of the Teacher: Some Light from the Past.

    ERIC Educational Resources Information Center

    Fortgang, Stephen J.

    1980-01-01

    Reviews the work of Howard S. Becker who, in the 1950s, analyzed teaching as a service occupation and drew parallels with other such occupations in terms of worker-client relationships and authority structures. (SJL)

  2. Are You Pregnant and Thinking about Adoption?

    MedlinePlus

    ... stories . O.J. Howard Publishing. Siegel, D.H., & Smith, S.L. (2012). Openness in adoption: From secrecy and ... from- secrecy- and- stigma- to- knowledge- and- connections/ Smith, S. (2007). Safeguarding the rights and well-being of ...

  3. That's Using Your Brain!

    ERIC Educational Resources Information Center

    Visser, Dana R.

    1996-01-01

    Discusses new adult learning theories, including those of Roger Sperry (left brain/right brain), Paul McLean (triune brain), and Howard Gardner (multiple intelligences). Relates adult learning theory to training. (JOW)

  4. 6. VIEW OF TUNNEL OPENING AT CAMDEN STATION (SOUTH END) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. VIEW OF TUNNEL OPENING AT CAMDEN STATION (SOUTH END) LOOKING OUT FROM A COUPLE OF HUNDRED FEET INSIDE TUNNEL. - Baltimore & Ohio Railroad, Howard Street Tunnel, 1300 Mount Royal Avenue, Baltimore, Independent City, MD

  5. 75 FR 104 - Manufacturing & Services' Sustainable Manufacturing Initiative; Update

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-04

    ... of Trade Policy Analysis, 202-482-2831. SUPPLEMENTARY INFORMATION: ITA's Manufacturing and Services... 18, 2009. Matthew Howard, Office of Trade Policy Analysis. BILLING CODE 3510-DR-P ... International Trade Administration Manufacturing & Services' Sustainable Manufacturing Initiative; Update...

  6. Neighborhood Revitalization: Achieving the Tipping Point.

    ERIC Educational Resources Information Center

    Digh, Patricia

    2002-01-01

    Describes how, partnering with Fannie Mae, three institutions--Howard University, the University of Pennsylvania, and Jackson State University--have transformed their deteriorating neighborhoods into thriving communities. (EV)

  7. An Extrapolation of Bowen's Revenue Theory of Cost to Academic Libraries.

    ERIC Educational Resources Information Center

    Leonard, W. Patrick

    1983-01-01

    This essay relates Howard R. Bowen's revenue theory of cost and Laws of Higher Education Cost to the situation of the academic library, highlighting pursuit of excellence, library expenses, cost-effective measures, and decision rules for resource allocation. (EJS)

  8. Think Before You Ink: Are Tattoos Safe?

    MedlinePlus

    ... time or fade when they are exposed to sunlight. And laser light is used to remove tattoos. “ ... water soluble, and out it goes,” says Howard. • Sunlight may cause the ink to break down so ...

  9. 1. ENVIRONMENT, FROM NORTHWEST, SHOWING B&P INTERLOCKING TOWER AND POWER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. ENVIRONMENT, FROM NORTHWEST, SHOWING B&P INTERLOCKING TOWER AND POWER SUBSTATIONS - Baltimore & Potomac Interlocking Tower, Adjacent to AMTRAK railroad tracks in block bounded by Howard Street, Jones Falls Expressway, Maryland Avenue & Falls Road, Baltimore, Independent City, MD

  10. 15. Detail of window area, showing WoodSpenceBrown 1901 lathe, second ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Detail of window area, showing Wood-Spence-Brown 1901 lathe, second floor looking south. - General Dynamics Corporation Shipyard, Joiner & Sheet Metal Shops, 97 East Howard Street, Quincy, Norfolk County, MA

  11. Chord Splicing & Joining Detail; Chord & CrossBracing Joint Details; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Chord Splicing & Joining Detail; Chord & Cross-Bracing Joint Details; Cross Bracing Center Joint Detail; Chord & Diagonal Joint Detail - Vermont Covered Bridge, Highland Park, spanning Kokomo Creek at West end of Deffenbaugh Street (moved to), Kokomo, Howard County, IN

  12. Who's Qualified? Seeing Race in Color-Blind Times: Lessons from Fisher v. University of Texas

    ERIC Educational Resources Information Center

    Donnor, Jamel K.

    2015-01-01

    Using Howard Winant's racial dualism theory, this chapter explains how race was discursively operationalized in the recent U.S. Supreme Court higher education antiracial diversity case Fisher v. University of Texas at Austin.

  13. 76 FR 70420 - New England Fishery Management Council (NEFMC); Public Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-14

    ... Water Street, Mill 2, Newburyport, MA 01950. FOR FURTHER INFORMATION CONTACT: Paul J. Howard, Executive... Sustainable Fisheries, National Marine Fisheries Service. BILLING CODE 3510-22-P ... National Oceanic and Atmospheric Administration RIN 0648-XA818 New England Fishery Management...

  14. At Last: A National Test of Taste and Smell | NIH MedlinePlus the Magazine

    MedlinePlus

    ... says Howard Hoffman, M.A., program director of epidemiology and statistics at the NIDCD, and one of ... well as brain disorders such as Parkinson's and Alzheimer's diseases, which are characterized by an early loss ...

  15. 11. INTERIOR DETAIL, BASEMENT, SHOWING CONDUITS LEADING UNDERGROUND TO SWITCHES ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. INTERIOR DETAIL, BASEMENT, SHOWING CONDUITS LEADING UNDERGROUND TO SWITCHES AND SIGNALS - Baltimore & Potomac Interlocking Tower, Adjacent to AMTRAK railroad tracks in block bounded by Howard Street, Jones Falls Expressway, Maryland Avenue & Falls Road, Baltimore, Independent City, MD

  16. 9. Photocopy of Blueprint (Original held by the Community end ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Photocopy of Blueprint (Original held by the Community end Economic Development Department, City of Detroit), C. Howard Crane, Architect. CROSS SECTION THROUGH STADIUM, NOVEMBER 27, 1926. (4' x 5' negative) - Olympia Arena, 5920 Grand River Avenue, Detroit, MI

  17. 7. Photocopy of Blueprint (Original held by the Community end ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Photocopy of Blueprint (Original held by the Community end Economic Development Department, City of Detroit), C. Howard Crane, Architect. FIRST FLOOR PLAN, NOVEMBER 13, 1927. (4' x 5' negative) - Olympia Arena, 5920 Grand River Avenue, Detroit, MI

  18. 19. Main deck just forward of fish batch, portion of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. Main deck just forward of fish batch, portion of fish hatch cover has been opened to show the ventilation grate below. - Schooner "Lettie G. Howard", South Street Seaport Museum, New York County, NY

  19. 15. VIEW LOOKING NORTH AT END POSTS WITH DIAGONAL TENSION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. VIEW LOOKING NORTH AT END POSTS WITH DIAGONAL TENSION MEMBERS. NOTE: MODERN VEHICULAR BRIDGE TO RIGHT. - Baltimore & Ohio Railroad, Bollman Truss Bridge, Spanning Little Patuxent River, Savage, Howard County, MD

  20. 4. Overview at top of crane showing crane bridge and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Overview at top of crane showing crane bridge and south truss full length, looking south east. - General Dynamics Corporation Shipyard, XYZ Crane & Towers, 97 East Howard Street, Quincy, Norfolk County, MA

  1. Scientists Reduce Alzheimer's-Linked Brain Plaques in Mice

    MedlinePlus

    ... progression. Dr. Howard Fillit, executive director and chief science officer at the Alzheimer's Drug Discovery Foundation, praised the study. "In more sophisticated and modern ways, it confirms previous knowledge" about how the ...

  2. Then & Now: Medical Research Pays Off for All Americans

    MedlinePlus

    ... Issues Then & Now Medical Research Pays Off for All Americans Past Issues / Fall 2007 Table of Contents ... which afflict tens of millions of Americans of all ages. William Howard Taft—Then & Now Taft's Condition ...

  3. 13. Photocopy of photograph (original in Documents Collection, College of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Photocopy of photograph (original in Documents Collection, College of Environmental Design, University of California, Berkeley, CA) Circa 1910, photographer unknown VIEW LOOKING NORTHEAST - Howard B. Gates House, 62 South Thirteenth Street, San Jose, Santa Clara County, CA

  4. A Photoacoustic Imaging System with Optimized Real-Time Parallel Reconstruction

    NASA Astrophysics Data System (ADS)

    Feng, Ting; Yuan, Jie; Yu, Yao; Zhou, Yu; Xu, Guan

    2013-10-01

    Biomedical photoacoustic tomography (PAT) provides anatomical, functional, metabolic, molecular, and genetic contrasts of vasculature, hemodynamics, oxygen metabolism, biomarkers, and gene expression. These attributes bring PAT to a wide variety of applications in clinical medicine and preclinical research. We report the development of a real-time PAT imaging system, which integrates signal scanning, image reconstruction and displaying photoacoustic images in real time. An optimized back projection algorithm for PAT imaging is proposed and tested on a latest graphics process unit based card. The whole system is built and tested in an experiment for monitoring moving blood events to validate the real-time performance of this system to image moving events.

  5. ISS Update: ATV-3’s Science Payload

    NASA Video Gallery

    ISS Update Commentator Pat Ryan interviews Camille Alleyne, Assistant Program Scientist for International Space Station. They discuss the science payload that was delivered to the station and crew ...

  6. ISS Update: Keeping the Flight Control Rooms Running

    NASA Video Gallery

    ISS Update Commentator Pat Ryan interviews Angela Bauer, Facilities Operations and Maintenance Group lead in the Mission Operations Directorate at Johnson Space Center. They discuss her group's rol...

  7. ISS Update: Burning and Suppression of Solids

    NASA Video Gallery

    ISS Update Commentator Pat Ryan interviews Paul Ferkul, Principal Investigator for the Burning and Suppression of Solids (BASS) experiment, about performing combustion experiments in microgravity. ...

  8. Protein S-ACYL Transferase10 is critical for development and salt tolerance in Arabidopsis.

    PubMed

    Zhou, Liang-Zi; Li, Sha; Feng, Qiang-Nan; Zhang, Yu-Ling; Zhao, Xinying; Zeng, Yong-lun; Wang, Hao; Jiang, Liwen; Zhang, Yan

    2013-03-01

    Protein S-acylation, commonly known as palmitoylation, is a reversible posttranslational modification that catalyzes the addition of a saturated lipid group, often palmitate, to the sulfhydryl group of a Cys. Palmitoylation regulates enzyme activity, protein stability, subcellular localization, and intracellular sorting. Many plant proteins are palmitoylated. However, little is known about protein S-acyl transferases (PATs), which catalyze palmitoylation. Here, we report that the tonoplast-localized PAT10 is critical for development and salt tolerance in Arabidopsis thaliana. PAT10 loss of function resulted in pleiotropic growth defects, including smaller leaves, dwarfism, and sterility. In addition, pat10 mutants are hypersensitive to salt stresses. We further show that PAT10 regulates the tonoplast localization of several calcineurin B-like proteins (CBLs), including CBL2, CBL3, and CBL6, whose membrane association also depends on palmitoylation. Introducing a C192S mutation within the highly conserved catalytic motif of PAT10 failed to complement pat10 mutants, indicating that PAT10 functions through protein palmitoylation. We propose that PAT10-mediated palmitoylation is critical for vacuolar function by regulating membrane association or the activities of tonoplast proteins. PMID:23482856

  9. Photoacoustic tomography of water in biological tissue

    NASA Astrophysics Data System (ADS)

    Xu, Zhun; Li, Changhui; Wang, Lihong V.

    2011-03-01

    As an emerging imaging technique that combines high optical contrast and ultrasonic detection, photoacoustic tomography (PAT) has been widely used to image optically absorptive objects in both human and animal tissues. PAT overcomes the depth limitation of other high-resolution optical imaging methods, and it is also free from speckle artifacts. To our knowledge, water has never been imaged by PAT in biological tissue. Here, for the first time, we experimentally imaged water in both tissue phantoms and biological tissues using a near infrared (NIR) light source. The differences among photoacoustic images of water with different concentrations indicate that laser-based PAT can usefully detect and image water content in tissue.

  10. Quantum and classical solutions for statically screened two-dimensional Wannier-Mott excitons

    SciTech Connect

    Makowski, Adam J.

    2011-08-15

    Quantum solutions and classical orbits are discussed for statically screened Wannier-Mott excitons for two closely related potentials: the Stern-Howard potential and a suggested simple focusing one. Bound states and exact ''quantized'' values of screening are obtained as well. For the suggested potential, the scattering matrix, the Regge poles, and the transmission coefficient are calculated exactly. We argue that the simple potential can be utilized in applications instead of the Stern-Howard potential, which is difficult to handle.

  11. The Infusaid Pump in the Management of Intractable Cancer Pain

    PubMed Central

    Bryant, D'Orsay D.; DeWitty, Robert L.; Dennis, Gary C.

    1987-01-01

    At Howard University Hospital, nine terminally ill cancer patients with chronic pain have been treated with continuous intrathecal infusion of morphine delivered by the implantable Infusaid pump. The case of a patient treated at Howard University Hospital with this method of pain management is presented. Following Infusaid pump insertion, the patient lived for 22 months and obtained substantial relief of his cancer pain with no adverse side effects. PMID:3573060

  12. An opto-chemical assay for mold detection in processing tomatoes

    NASA Astrophysics Data System (ADS)

    Potts, Steven James

    Fungal (mold) contamination is an important indicator of low quality raw product or unsanitary processing conditions in the food industry. A quantitative lectin assay was developed that was less expensive, faster, and more precise than the industry standard Howard mold count. This assay, based on a fluorescent-labeled lectin isolated from wheat germ, has a selective affinity for the chitin in fungal cell walls. Assay values had high linear correlations (from r 2 = .72 to r2 = .99) with fungal biomass for ten fungal species of greatest importance to the California processing tomato industry. One hundred raw tomato juice samples with natural mold infections were collected, as part of the normal California processing tomato inspection program, from commercial processing tomato loads. The raw juice samples were sent to four processor quality control laboratories, where an industry standard Howard mold count was conducted on blind triplicates of each of the samples. The lectin assay was also conducted on blind replicates of the raw juice samples. The correlation between the lectin assay and the Howard mold count (r = .85) was as strong as the correlation between the Howard mold counts for two facilities. The assay had significantly better (alpha = .01) precision than the Howard mold count, with an average coefficient of variation of 8%, compared with 38% for the Howard mold count. The assay is objective, can be conducted in under six minutes, and has the potential to replace mold testing in both raw and processed products.

  13. Hearing on the Reauthorization of the Juvenile Justice and Delinquency Prevention Act. Hearing before the Subcommittee on Human Resources of the Committee on Education and Labor. House of Representatives, One Hundred Second Congress, Second Session.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and Labor.

    Testimony from a hearing on the Reauthorization of the Juvenile Justice and Delinquency Prevention Act is presented in this document. Comments and a prepared statement by Representative Matthew G. Martinez open the document. Testimony from Representatives Harris W. Fawell and Dale E. Kildee is also included. Testimony and/or prepared statements…

  14. Oversight Hearing on the Missing Children's Assistance Act. Hearing before the Subcommittee on Human Resources of the Committee on Education and Labor. House of Representatives, Ninety-Ninth Congress, Second Session.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and Labor.

    This document contains testimonies and prepared statements from the Congressional oversight hearing called to examine the implementation and operation of the Missing Children's Assistance Act of 1984. Opening statements are included by Representatives Dale Kildee and Thomas Tauke. Witnesses providing testimony include: (1) Verne Speirs and…

  15. Readability Formulas--Fact or Fiction.

    ERIC Educational Resources Information Center

    Olson, Arthur V.

    Four readability formulas were analyzed to determine the reading grade equivalence of instructional materials at the middle grades and above: the Dale-Chall formula, the Gunning-Fog Index, the Flesch Reading Ease Formula, and the McLaughlin SMOG Grading. In addition, Spache and Wheeler/Smith formulas were analyzed for evaluating primary grade…

  16. A Quick Guide to Readability Formulas.

    ERIC Educational Resources Information Center

    Cramer, Eugene H.

    1978-01-01

    Six of the most widely used readability scales are briefly reviewed and their original source cited: the Dale-Chall Readability Formula, the Flesch "Reading Ease" Formula, the Fog Index, the Fry Readability Graph, the SMOG Grading Plan, and the Spache Readability Formula. (SJL)

  17. Student and Faculty Perceptions of Plus/Minus Grading and Its Effect on Course Grade Point Averages

    ERIC Educational Resources Information Center

    Edgar, Leslie D.; Johnson, Donald M.; Graham, Donna L.; Dixon, Bruce L.

    2014-01-01

    In fall 2005, the Dale Bumpers College of Agricultural, Food and Life Sciences at the University of Arkansas authorized the optional use of a plus/minus grading system. Since 2005, approximately one-half of courses have been graded using plus/minus and one-half using the straight letter grade system. This study examined student (n = 338) and…

  18. Reauthorization Hearings on the Older Americans Act: Part 2. Hearings before the Subcommittee on Human Resources of the Committee on Education and Labor. House of Representatives, One Hundredth Congress (Montpelier, VT, March 28; Flint, MI, April 11; and Algona, IA, April 24, 1987).

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and Labor.

    This document contains the text of three hearings held in Vermont, Michigan, and Iowa which constitute part two of the Congressional hearings held to examine reauthorization of the Older Americans Act. Opening statements are included from Representatives Dale Kildee, Tom Sawyer, Fred Grandy, and Thomas Tauke. Testimony is provided from 55…

  19. On JALT 95: Curriculum and Evaluation. Proceedings of the JALT International Conference on Language Teaching/Learning (22nd, Nagoya, Japan, November 1995). Section Seven: Testing and Evaluation.

    ERIC Educational Resources Information Center

    Brown, James Dean; And Others

    This section contains conference papers on testing and evaluation in second language teaching. They include: "English Language Entrance Examinations in Japan: Problems and Solutions" (James Dean Brown); "Reliability and a Learner Style Questionnaire" (Dale T. Griffee); "Does It Work?" Evaluating Language Learning Tasks" (Rod Ellis); "Communicative…

  20. Dashboard Videos

    ERIC Educational Resources Information Center

    Gleue, Alan D.; Depcik, Chris; Peltier, Ted

    2012-01-01

    Last school year, I had a web link emailed to me entitled "A Dashboard Physics Lesson." The link, created and posted by Dale Basier on his "Lab Out Loud" blog, illustrates video of a car's speedometer synchronized with video of the road. These two separate video streams are compiled into one video that students can watch and analyze. After seeing…