Sample records for ikh ehlementnogo sostava

  1. Development and validation of a LC-MS assay for the quantification of ikh12 a novel anti-tumor candidate in rat plasma and tissues and its application in a pharmacokinetic study.


    Otaegui, Dorleta; Masdeu, Carme; Aldaba, Eneko; Vara, Yosu; Zubia, Aizpea; San Sebastian, Eider; Alcalá, Maria; Villafruela, Sergio; Cossío, Fernando P; Rodriguez-Gascón, Alicia


    IKH12 is a novel histone deacetylase 6 selective inhibitor. A rapid and sensitive liquid chromatography tandem mass spectrometry method was developed and validated for the quantification of IKH12 in rat plasma and tissue with kendine 91 as internal standard (IS). The samples were prepared by liquid-liquid extraction with tert-butyl methyl ether. The chromatographic separation was accomplished by using a Zorbax Extend C18 4.6 × 150 mm, 5 µm column, with a mobile phase consisting of methanol and 0.1% formic acid (75:25 v/v). Multiple reaction monitoring, using electrospray ionization in positive ion mode, was employed to quantitatively detect IKH12 and IS. The monitored transitions were set at m/z 418 → 252 and 444 → 169 for IKH12 and kendine 91, respectively. The calibration curve was linear over the concentration range 2-1000 ng mL(-1) . The intra- and inter-assay precision and accuracy of the quality controls and the limit of quantification were satisfactory in all cases (according to European Medicines Agency guidelines). Stability studies showed that plasma samples were stable in the chromatography rack for 24 h and at -80°C for 2 months and also after three freeze-thaw cycles. This method was successfully applied to a pharmacokinetic study of IKH12 in rat. PMID:25616154

  2. Recognition, assessment and management of hypoglycaemia in childhood.


    Ghosh, Arunabha; Banerjee, Indraneel; Morris, Andrew A M


    Hypoglycaemia is frequent in children and prompt management is required to prevent brain injury. In this article we will consider hypoglycaemia in children after the neonatal period. The most common causes are diabetes mellitus and idiopathic ketotic hypoglycaemia (IKH) but a number of endocrine disorders and inborn errors of metabolism (IEMs) need to be excluded. Elucidation of the diagnosis relies primarily on investigations during a hypoglycaemic episode but may also involve biochemical tests between episodes, dynamic endocrine tests and molecular genetics. Specific treatment such as cortisol replacement and pancreatic surgery may be required for endocrine causes of hypoglycaemia, such as adrenal insufficiency and congenital hyperinsulinism. In contrast, in IKH and most IEMs, hypoglycaemia is prevented by limiting the duration of fasting and maintaining a high glucose intake during illnesses. PMID:26718813

  3. A comprehensive constitutive law for waxy crude oil: a thixotropic yield stress fluid.


    Dimitriou, Christopher J; McKinley, Gareth H


    Guided by a series of discriminating rheometric tests, we develop a new constitutive model that can quantitatively predict the key rheological features of waxy crude oils. We first develop a series of model crude oils, which are characterized by a complex thixotropic and yielding behavior that strongly depends on the shear history of the sample. We then outline the development of an appropriate preparation protocol for carrying out rheological measurements, to ensure consistent and reproducible initial conditions. We use RheoPIV measurements of the local kinematics within the fluid under imposed deformations in order to validate the selection of a particular protocol. Velocimetric measurements are also used to document the presence of material instabilities within the model crude oil under conditions of imposed steady shearing. These instabilities are a result of the underlying non-monotonic steady flow curve of the material. Three distinct deformation histories are then used to probe the material's constitutive response. These deformations are steady shear, transient response to startup of steady shear with different aging times, and large amplitude oscillatory shear (LAOS). The material response to these three different flows is used to motivate the development of an appropriate constitutive model. This model (termed the IKH model) is based on a framework adopted from plasticity theory and implements an additive strain decomposition into characteristic reversible (elastic) and irreversible (plastic) contributions, coupled with the physical processes of isotropic and kinematic hardening. Comparisons of experimental to simulated response for all three flows show good quantitative agreement, validating the chosen approach for developing constitutive models for this class of materials. PMID:25008187

  4. Ecosystem based river basin management planning in critical water catchment in Mongolia

    NASA Astrophysics Data System (ADS)

    Tugjamba, Navchaa; Sereeter, Erdenetuul; Gonchigjav, Sarantuya


    Developing the ecosystem based adaptation strategies to maintain water security in critical water catchments in Mongolia would be very significant. It will be base by reducing the vulnerability. "Ecosystem Based adaptation" is quite a new term in Mongolia and the ecosystem approach is a strategy for the integrated management of land, water and living resources that promotes conservation and sustainable use in an equitable way. To strengthen equitable economic development, food security, climate resilience and protection of the environment, the implementation of sustainable river basin management in critical water catchments is challenging in Mongolia. The Ulz river basin is considered one of the critical water catchments due to the temperature has increased by in average 1.30Ñ over the period 1976 to 2011. It is more intense than the global warming rate (0.740C/100 years) and a bit higher than the warming rate over whole Mongolia as well. From long-term observations and measurements it is clear that Ulz River has low water in a period of 1970-1980 and since the end of 1980s and middle of 1990s there were dominated years of the flood. However, under the influence of the global warming, climate changes of Mongolia and continuation of drought years with low water since the end of 1990s until today river water was sharply fallen and dried up. For the last ten years rivers are dried up and annual mean run-off is less by 3-5 times from long term mean value. The Ulz is the transboundary river basin and taking its origin from Ikh and Baga Burd springs on territory of Norovlin soum of Khentii province that flows through Khentii and Dornod provinces to the northeast, crossing the state border it flows in Baruun Tari located in Tari Lake concavity in Russia. Based on the integrative baseline study on the 'The Ulz River Basin Environmental and Socioeconomic condition', ecosystem based river basin management was planned. 'Water demand Calculator 3' (WDC) software was used to

  5. Geomorphological development of Eastern Mongolian plain, Mongolia

    NASA Astrophysics Data System (ADS)

    khukhuudei, Ulambadrakh; otgonbayar, Orolzodmaa


    - K-Ar age for Mesozoic volcanic rocks from Eastern Mongolia and Ikh Khyangan dated in range from 160 to 110Ma (Shuvalov, 1987; Makhbadar et al., 1995; Zhou et al., 2006; Bat-Ulzii, 2014). 2. Rift controlling fault - Delgerekh, Modon Ovoo and Matad faults to north-east direction are interpreted and presented in EMP (Khasin, 1973; Tikhonov, 1974; Byamba, 2009). 3. Basin and Range topography - we are interpreting that Nukhet davaa mountain range, Tamsag basin, Matad uplift or range and Choibalsan basin were developed in that time. Hills with 40-150 m height at a present day are interpreted that they developed from the Late Cretaceous period. In post- rift or Late Cretaceous period, lakes disappeared and whole area of Eastern Mongolia became a dry land, dominating by exogenic process. Denudation planation surface at EMP are kept in high and low level. High or old planation surface is proposed by age of the Miocene - Lower Pliocene and low or young planation surface - Late Pliocene. Just a current topography of EMP is formed in Late Neogene - Lower Quaternary period as a peneplain.