Science.gov

Sample records for imaging program cip

  1. Health Sciences. Program CIP: 51.0000

    ERIC Educational Resources Information Center

    Murdock, Ashleigh, Ed.

    2007-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  2. Polymer Science. Program CIP: 15.0607

    ERIC Educational Resources Information Center

    Research and Curriculum Unit, 2010

    2010-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  3. Marketing. Program CIP: Marketing: 52.1801

    ERIC Educational Resources Information Center

    Murdock, Ashleigh Barbee, Ed.

    2008-01-01

    Secondary career-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  4. Specialized Initiatives - Cancer Imaging Program

    Cancer.gov

    CIP has sponsored a number of programs for specific purposes, using set-aside funds. Among these are Phase 2 N01 ProgramIn-Vivo Cellular & Molecular Imaging Centers (ICMICs) Quantitative Imaging for Evaluation of Responses to Cancer Therapies (QIN) Network for Translational Research (NTR): Optical Imaging in Multimodal Platforms Small Animal Imaging Resource Program (SAIRP) Development of Preclinical Drugs and Enhancers (DCIDE) program.

  5. Efficacy of the Social Skills Improvement System Classwide Intervention Program (SSIS-CIP) Primary Version

    ERIC Educational Resources Information Center

    DiPerna, James Clyde; Lei, Puiwa; Bellinger, Jillian; Cheng, Weiyi

    2015-01-01

    A multisite cluster randomized trial was conducted to examine the effects of the Social Skills Improvement System Classwide Intervention Program (SSIS-CIP; Elliott & Gresham, 2007) on students' classroom social behavior. The final sample included 432 students across 38 second grade classrooms. Social skills and problem behaviors were measured…

  6. Single Institution Feasibility Trials - Cancer Imaging Program

    Cancer.gov

    Within the CIP program, the current R21 mechanism provides potential funding for small, single institution feasibility trials. The current announcement is titled In Vivo Cancer Imaging Exploratory/Developmental Grants.

  7. Phase 2 N01 Program - Cancer Imaging Program

    Cancer.gov

    The Phase 2 N01 Program is a CTEP-CIP collaboration includes 7 contractors, most of whom consist of multi-institutional consortia, and includes a total of 22 NCI-designated Cancer Centers. These sites carry out early clinical trials with CTEP and CIP-held IND agents, with an emphasis on phase 2 trials, but including phase 1 trials as well. These trials include the evaluation of novel imaging agents and methods to enhance the evaluation of novel therapeutics.

  8. Business Fundamentals. Program CIP: Business Fundamentals: 52.0101

    ERIC Educational Resources Information Center

    Murdock, Ashleigh Barbee, Ed.

    2008-01-01

    Secondary career-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  9. Installation and Service: HVAC. Program CIP: 47.0201

    ERIC Educational Resources Information Center

    Research and Curriculum Unit, 2009

    2009-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  10. Simulation and Animation Design. Program CIP: 50.0411

    ERIC Educational Resources Information Center

    Murdock, Ashleigh Barbee, Ed.

    2010-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  11. Digital Media Technology. Program CIP: 09.0702

    ERIC Educational Resources Information Center

    Agee, Kelly, Ed.

    2009-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  12. Early Childhood Education. Program CIP: 19.0709

    ERIC Educational Resources Information Center

    Murdock, Ashleigh Barbee, Ed.

    2010-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  13. Welding Technology. Program CIP: 48.0508 - WELDING

    ERIC Educational Resources Information Center

    Ferguson, Doug

    2010-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  14. Automotive Service Technician. Program CIP: 47.0604 - Transportation

    ERIC Educational Resources Information Center

    Agee, Kelly, Ed.

    2008-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  15. Culinary Arts. Program CIP: 12.0500-Culinary Arts

    ERIC Educational Resources Information Center

    Murdock, Ashleigh, Ed.

    2008-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  16. Management. Program CIP: Business Management: 52.0204

    ERIC Educational Resources Information Center

    Murdock, Ashleigh Barbee, Ed.

    2008-01-01

    Secondary career-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  17. Industrial Maintenance. Program CIP: 47.0303 - Industrial Maintenance

    ERIC Educational Resources Information Center

    Research and Curriculum Unit, 2009

    2009-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  18. Diesel Service Technician. Program CIP: 47.0605

    ERIC Educational Resources Information Center

    Agee, Kelly, Ed.

    2010-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  19. Mesospheric Zonal Mean Winds Derived from Consecutive Orbits of AIM Cips Images

    NASA Astrophysics Data System (ADS)

    Rong, P. P.; Yue, J.; Russell, J. M., III; Lumpe, J. D., Jr.; Gong, J.; Wu, D. L.; Randall, C. E.

    2014-12-01

    In order to infer mesospheric wind velocities, polar mesospheric cloud (PMC) pattern variations are investigated using images from consecutive orbits taken by the Cloud Imaging and Particle Size instrument (CIPS) aboard the AIM satellite. CIPS measurements are analyzed to detect patterns that repeat from one orbit to the next, but are displaced in location; the displacement provides a measure of the wind velocity. Pattern matching is achieved by re-sampling the CIPS data to a standard geographic grid with a horizontal resolution of 0.2° longitude × 0.05° latitude (~25 km2), and correlating patterns within geographic frames of size 24° longitude × 3.6° latitude. Such a frame size is arbitrarily chosen, but it covers a hierarchy of cloud structures including scales as large as several hundred kilometers. A relatively larger frame is required because after ~90 minutes, the time of one orbit, the smaller scale features are no longer conserved. Several thousand pairs, taken from 10-14 July 2007, are matched to derive the statistics. These pairs are mostly evenly distributed at longitudes and latitudes north of 70⁰N for each given day. The results suggest that the zonal velocity probability distribution during this 5-day period was peaked at around -40m/s with a 1-σ scatter of ~35m/s. The meridional velocity distribution peaked at 0 m/s with a 1-σ scatter of ~25m/s. These prevailing velocities can be determined with high precision because the corresponding patterns are shifted by at least half of the frame size from one orbit to the next. The CIPS cloud albedo on consecutive orbits is also examined for variations at fixed locations. The statistical results suggest that the mean cloud albedo within a given frame will most likely be weakened or strengthened by < 30% on consecutive orbits, although larger variations can occur with lower probability. Such a conclusion applies to both bright and dim clouds. This indicates that within 90 minutes the cloud brightness

  20. Mississippi Curriculum Framework for Horticulture Technology Cluster (Program CIP: 01.0601--Horticulture Serv. Op. & Mgmt., Gen.) (Program CIP: 01.0605--Landscaping Op. & Mgmt.). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the horticulture technology programs cluster. Presented in the introductory section are a framework of programs and courses, description of the programs, and suggested course sequences for…

  1. Mississippi Curriculum Framework for Drafting and Design Technology (Program CIP: 48.0102--Architectural Drafting Technology) (Program CIP: 48.0101--General Drafting). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the two course sequences of the state's postsecondary-level drafting and design technology program: architectural drafting technology and drafting and design technology. Presented first are a program description and…

  2. Mississippi Curriculum Framework for Emergency Medical Technology--Basic (Program CIP: 51.0904). Emergency Medical Technology--Paramedic (Program CIP: 51.0904). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the emergency medical technology (EMT) programs cluster. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline…

  3. Mississippi Curriculum Framework for Residential Carpentry Technology (Program CIP: 46.0201--Carpenter). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for certificate of residential carpentry and residential carpentry technology programs. Presented in the introductory section are program descriptions and suggested course sequences for both programs. Section I lists…

  4. Information Systems - Cancer Imaging Program

    Cancer.gov

    The Lung Image Database Consortium (LIDC) represents an effort by CIP grantees in a consortium to create a database of spiral CT images of the lung for use in CAD (computer-aided detection) algorithm research. The Imaging Database Resources Initiative (IDRI) is extending the efforts of the LIDC, to create a larger database of spiral CT imaging of the lung for use in CAD algorithm research. Image Archive Resources contains links to Web sites related to the interests of the NCI CIP Image Archive Committee. The Molecular Imaging and Contrast Agent Database (MICAD) is a database of research data on in vivo molecular imaging and contrast agents.

  5. Mississippi Curriculum Framework for Welding (Program CIP: 48.0508--Welder/Welding Technologist). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for welding I and II. Presented first are a program description and course…

  6. Mississippi Curriculum Framework for Agriscience (Program CIP: 02.0101--Agriculture Science). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for agriscience I and II. Presented first are a program description and…

  7. Mississippi Curriculum Framework for Automotive Machinist (Program CIP: 47.0690--Auto Machinist). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the automotive machinist programs cluster. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies, and…

  8. Mississippi Curriculum Framework for Allied Health (Program CIP: 51.1699--Nursing, Other). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for allied health I and II. Presented first are a program description and…

  9. Mississippi Curriculum Framework for Building Trades (Program CIP: 46.0490--Building Trades, General). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for two secondary-level courses in the building trades: building trades I and…

  10. Mississippi Curriculum Framework for Residential Carpentry (Program CIP: 46.0201--Carpenter). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for two secondary-level courses in carpentry: carpentry I and II. Presented…

  11. Mississippi Curriculum Framework for Electronics (Program CIP: 47.0190--Electronics (Secondary)). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for two secondary-level courses in electronics: electronics I and II.…

  12. Mississippi Curriculum Framework for Electrician (Program CIP: 46.0302--Electrician). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for secondary-level courses to prepare Mississippi vocational students for…

  13. Mississippi Curriculum Framework for General Drafting (Program CIP: 48.0101--Drafting, General). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for two secondary-level courses in drafting: drafting I and II. Presented…

  14. Mississippi Curriculum Framework for Metal Trades (Program CIP: 48.0590--Metal Trades). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for metal trades I, IIA (advanced welding), and IIB (advanced machine shop).…

  15. Mississippi Curriculum Framework for Sheet Metal Programs (Program CIP: 48.0506--Sheet Metal Worker). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document provides the framework for a postsecondary sheet metal program of instruction. A foreword provides guidelines that were used in developing the program and should be considered in compiling and revising course syllabi and daily lesson plans at the local level. A description of the sheet metal programs (building trades sheet metal work…

  16. Mississippi Curriculum Framework for Banking & Finance Technology (Program CIP: 52.0803--Banking and Related Financial Programs, Other). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the banking and finance technology program. Presented in the introduction are a program description and suggested course sequence. Section I is a curriculum guide consisting of outlines for…

  17. Mississippi Curriculum Framework for Dental Assisting Technology Programs (Program CIP: 51.0601--Dental Assistant). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the dental assisting technology program. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies. Section II…

  18. Mississippi Curriculum Framework for Welding and Cutting Programs (Program CIP: 48.0508--Welder/Welding Technologist). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the welding and cutting programs cluster. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies, and…

  19. Mississippi Curriculum Framework for Physical Therapist Assistant (CIP: 51.0806--Physical Therapy Assistant). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the physical therapy assistant program. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies, and section…

  20. Mississippi Curriculum Framework for Dental Hygiene Technology (Program CIP: 51.0602--Dental Hygienist). Postsecondary Education.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the dental hygiene technology program. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies. Section II…

  1. CIPS Validation Data Plan

    SciTech Connect

    Nam Dinh

    2012-03-01

    This report documents analysis, findings and recommendations resulted from a task 'CIPS Validation Data Plan (VDP)' formulated as an POR4 activity in the CASL VUQ Focus Area (FA), to develop a Validation Data Plan (VDP) for Crud-Induced Power Shift (CIPS) challenge problem, and provide guidance for the CIPS VDP implementation. The main reason and motivation for this task to be carried at this time in the VUQ FA is to bring together (i) knowledge of modern view and capability in VUQ, (ii) knowledge of physical processes that govern the CIPS, and (iii) knowledge of codes, models, and data available, used, potentially accessible, and/or being developed in CASL for CIPS prediction, to devise a practical VDP that effectively supports the CASL's mission in CIPS applications.

  2. 2006 Mississippi Curriculum Framework: Secondary Forestry. (Program CIP: 03.0511 - Forestry Technology/Technician)

    ERIC Educational Resources Information Center

    Research and Curriculum Unit, 2006

    2006-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  3. 2005 Mississippi Curriculum Framework: Secondary Building Trades. (Program CIP: 46.0000 - Construction Trades, General)

    ERIC Educational Resources Information Center

    Jernigan, Jarvis; Manning, Phillip; Matkins, Billy

    2005-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  4. Construction: Carpentry Concentration. Program CIP: 46.0000 Construction: Carpentry Concentration

    ERIC Educational Resources Information Center

    Agee, Kelly, Ed.

    2008-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  5. 2005 Mississippi Curriculum Framework: Secondary Cosmetology. (Program CIP: 12.0401 - Cosmetology/Cosmetologist, General)

    ERIC Educational Resources Information Center

    Buchanon, Rouser; Farmer, Helen

    2005-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  6. 2005 Mississippi Curriculum Framework: Secondary Metal Trades. (Program CIP: 48.0590 - Metal Trades)

    ERIC Educational Resources Information Center

    Brown, Gary; Sample, John; Waits, Jeffrey; Britt, Albert; McKee, Steve; Sullivan, Kirk; Warren, Brian

    2005-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  7. 2005 Mississippi Curriculum Framework: Secondary Aging Services. (Program CIP: 19.0702 - Adult Development and Aging)

    ERIC Educational Resources Information Center

    Walker, Kathy

    2005-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  8. 2007 Mississippi Curriculum Framework: Secondary Introduction to Agriscience. (Program CIP: 01.0001 - Introduction to Agriscience)

    ERIC Educational Resources Information Center

    Research and Curriculum Unit, 2007

    2007-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  9. 2007 Mississippi Curriculum Framework: Secondary Agriscience. (Program CIP: 01.0000 - Agriculture, General)

    ERIC Educational Resources Information Center

    Cantrell, Steve; Conway, Scott; Jack, Linda; Stuckey, Dan

    2007-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  10. Information Technology. Program CIP: 11.0101 Computer Technology/Computer Systems Technology

    ERIC Educational Resources Information Center

    Agee, Kelly, Ed.

    2009-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  11. 2005 Mississippi Curriculum Framework: Secondary Masonry. (Program CIP: 46.0101 - Mason/Masonry)

    ERIC Educational Resources Information Center

    Davis, Milton; Harris, Chester; Richards, Toney; Smith, Allen; Weatherly, Ronald; Weeks, W. D.

    2005-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  12. 2007 Mississippi Curriculum Framework: Secondary Computer Graphics. (Program CIP: 50.0402 - Commercial and Advertising Art)

    ERIC Educational Resources Information Center

    Creel, Jo Anne; Denson, Cornelius; New, Ray

    2007-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  13. Mississippi Curriculum Framework for Surgical Technology Programs (CIP: 51.0909--Surgical/Operating Room Tech.). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the surgical technology program. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies for the program,…

  14. Mississippi Curriculum Framework for Forestry Technology (Program CIP: 03.0401--Forest Harvesting and Production Technology). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the forestry technology program cluster. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies for the…

  15. Mississippi Curriculum Framework for Fashion Marketing Technology (Program CIP: 08.0101--Apparel and Accessories Mkt. Op., Gen.). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the fashion marketing technology programs cluster. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies,…

  16. Mississippi Curriculum Framework for Automotive Mechanics (Program CIP: 47.0604--Auto/Automotive Mechanic/Tech). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for automotive mechanics I and II. Presented first are a program description…

  17. Mississippi Curriculum Framework for Brick, Block, and Stonemasonry (Program CIP: 46.0101--Mason and Tile Setter). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the brick, block, and stonemasonry program. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies for the…

  18. Mississippi Curriculum Framework for Ophthalmic Technology (Program CIP: 51.1801--Opticianry/Dispensing Optician). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the ophthalmic technology program. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies, and section II…

  19. Mississippi Curriculum Framework for Civil Technology (Program CIP: 15.0201--Civil Engineering/Civil Technology). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the civil technology programs cluster. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies, and section…

  20. Mississippi Curriculum Framework for Practical Nursing Programs CIP: 51.1613--Practical Nurse (L.P.N. Training). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the practical nursing program. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies, and section II…

  1. Mississippi Curriculum Framework for Introduction to Agriscience (Program CIP: 02.9990--Introduction to Agriculture Science). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for the introduction to agriscience program. Presented first are a program…

  2. Mississippi Curriculum Framework for Veterinary Technology (Program CIP: 51.0808--Veterinarian Asst./Animal Health). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the veterinary technology program. Presented in the introductory section are a of the program and suggested course sequence. Section I lists baseline competencies, and section II consists of…

  3. Mississippi Curriculum Framework for Postsecondary Child Development Technology Programs (CIP: 20.0201--Child Care & Guidance Workers & Mgr). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the child development technology programs cluster. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies,…

  4. Mississippi Curriculum Framework for Postsecondary Teacher Assistant Programs (CIP: 13.1501--Teacher Assistant/Aide). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the teacher assistant program. Presented in the introduction are a program description and suggested course sequence. Section I is a curriculum guide consisting of outlines for each of the…

  5. Mississippi Curriculum Framework for Collision Repair Technology (Program CIP: 47.0603--Auto/Autobody Repair). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the collision repair technology programs cluster. Presented in the introductory section are a description of the program and suggested course sequences for 1- and 2-year certificates. Section…

  6. Mississippi Curriculum Framework for Respiratory Care Technology Programs (CIP: 51.0908--Respiratory Therapy Technology). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the respiratory care technology program. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies, and…

  7. Mississippi Curriculum Framework for Automotive Technology Programs (CIP: 47.0604--Automotive Mechanic/Tech.). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the automotive technology programs cluster. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies, and…

  8. Mississippi Curriculum Framework for Plumber and Pipefitter/Steamfitter (Program CIP: 46.0501--Plumber and Pipefitter). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the plumber and pipefitter/steamfitter cluster. Presented in the introductory section are program descriptions and suggested course sequences for the plumbing and pipefitting programs. Section…

  9. Mississippi Curriculum Framework for Horticulture (Program CIP: 01.0601--Horticulture Serv. Op. & Mgmt., Gen.). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for horticulture I and II. Presented first are a program description and…

  10. Mississippi Curriculum Framework for Forestry (Program CIP: 03.0401--Forest Harvesting and Production Technology). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for forestry I and II. Presented first are a program description and course…

  11. Mississippi Curriculum Framework for Medical Laboratory Technology Programs (CIP: 51.1004--Medical Laboratory Technology). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the medical laboratory technology program. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies, and…

  12. Mississippi Curriculum Framework for Marketing Management Technology (Program CIP: 52.1401--Business Mkt. & Mkt. Mgmt.). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the state's marketing management technology program. Presented in the introduction are a program description and suggested course sequence. Section I lists baseline competencies for the…

  13. Programmed cell death protein 4 suppresses CDK1/cdc2 via induction of p21(Waf1/Cip1).

    PubMed

    Göke, R; Barth, P; Schmidt, A; Samans, B; Lankat-Buttgereit, B

    2004-12-01

    We show that the recently discovered tumor suppressor pdcd4 represses the transcription of the mitosis-promoting factor cyclin-dependent kinase (CDK)1/cdc2 via upregulation of p21(Waf1/Cip1). p21(Waf1/Cip1) inhibits CDK4/6 and CDK2. Decrease of CDK4/6 and CDK2 enhances the binding of pRb to E2F/DP, which in turn together bind to and repress the cdc2 promoter. Upregulation of CDK1/cdc2 accompanied by a malignant change was previously reported in colon cancer. We show that expression of pdcd4 as an indirect suppressor of CDK1/cdc2 is lost in progressed carcinomas of lung, breast, colon, and prostate. Furthermore, it seems that localization and expression of pdcd4 directly correlate with tumor progression. Finally, the CDK1/cdc2 inhibitor roscovitine reduces the proliferation of several tumor cell lines, suggesting that inhibition of CDK1/cdc2 may be a useful strategy against malignant transformation. Therefore, pdcd4 might serve as a novel target for antineoplastic therapies. PMID:15317660

  14. Mississippi Curriculum Framework for Agriculture Production (Program CIP: 01.0301--Agricultural Prod. Workers & Mgrs.). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for agriculture production I and II. Presented first are a program…

  15. Mississippi Curriculum Framework for Agriculture Business and Management (Program CIP: 01.0101--Agriculture Business & Mgmt., Gen.). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for agriculture business and management (ABM) I and II. Presented first are a…

  16. Mississippi Curriculum Framework for Diesel Equipment Repair & Service (Program CIP: 47.0605--Diesel Engine Mechanic & Repairer). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for diesel engine mechanics I and II. Presented first are a program…

  17. Mississippi Curriculum Framework for Automotive Body Repair (Program CIP: 47.0603--Auto/Automotive Body Repairer). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for automotive body repair I and II. Presented first are a program…

  18. Mississippi Curriculum Framework for Marketing and Fashion Merchandising (Program CIP: 08.0705--General Retailing Operations). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for marketing I-II and fashion merchandising. Presented first are a program…

  19. Mississippi Curriculum Framework for Brick, Block, and Stonemasonry (Program CIP: 46.0101--Mason and Tile Setter). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for two secondary-level courses in brick, block, and stonemasonry: brick,…

  20. Mississippi Curriculum Framework for Machine Tool Operation/Machine Shop (Program CIP: 48.0503--Machine Shop Assistant). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for machine tool operation/machine shop I and II. Presented first are a…

  1. Mississippi Curriculum Framework for Graphics and Print Communications (Program CIP: 48.0201--Graphic and Printing Equipment Operators). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for secondary-level courses in graphics and print communications I-II.…

  2. Mississippi Curriculum Framework for Computer Information Systems Technology. Computer Information Systems Technology (Program CIP: 52.1201--Management Information Systems & Business Data). Computer Programming (Program CIP: 52.1201). Network Support (Program CIP: 52.1290--Computer Network Support Technology). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for two programs in the state's postsecondary-level computer information systems technology cluster: computer programming and network support. Presented in the introduction are program descriptions and suggested course…

  3. Mississippi Curriculum Framework for Machine Tool Operation/Machine Shop and Tool and Die Making Technology Cluster (Program CIP: 48.0507--Tool and Die Maker/Technologist) (Program CIP: 48.0503--Machine Shop Assistant). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the machine tool operation/machine tool and tool and die making technology programs cluster. Presented in the introductory section are a framework of courses and programs, description of the…

  4. Mississippi Curriculum Framework for Agricultural Business and Management Technology Cluster: Agricultural Business and Management Technology (CIP: 01.0302--Agricultural Animal Husbandry/Prod) (CIP: 01.0501--Agric Supplies Retail & Wholesale); Animal Husbandry Technology (CIP: 01.0302--Agricultural Animal Husbandry/Prod); Field Crops Technology (CIP: 01.0104--Farm & Ranch Management). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the agricultural business and management technology programs cluster. Presented in the introductory section are a framework of courses and concentrations, a description of the program, and…

  5. XVD Image Display Program

    NASA Technical Reports Server (NTRS)

    Deen, Robert G.; Andres, Paul M.; Mortensen, Helen B.; Parizher, Vadim; McAuley, Myche; Bartholomew, Paul

    2009-01-01

    The XVD [X-Windows VICAR (video image communication and retrieval) Display] computer program offers an interactive display of VICAR and PDS (planetary data systems) images. It is designed to efficiently display multiple-GB images and runs on Solaris, Linux, or Mac OS X systems using X-Windows.

  6. Mississippi Curriculum Framework for Medical Radiologic Technology (Radiography) (CIP: 51.0907--Medical Radiologic Technology). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the radiologic technology program. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies for the program,…

  7. Mississippi Curriculum Framework for Diesel Equipment Technology (CIP: 47.0605--Diesel Engine Mechanic & Repairer). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the diesel equipment technology programs cluster. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies,…

  8. Mississippi Curriculum Framework for Postsecondary Health Information Technology (CIP: 51.0707--Medical Records Technol./Tech.). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the health information technology program. Presented in the introductory section are a description of the program and suggested course sequence. Section I lists baseline competencies, and…

  9. DCIDE - Cancer Imaging Program

    Cancer.gov

    DCIDE is no longer taking applications, as of August 2009. Imaging drug development resources should be requested through the new NExT/CBC program which has its own application and review process. See NCI Experimental Therapeutics Program (NExT). There are 4 review dates per year, instead of the previous DCIDE submission and review process.

  10. 2002 Mississippi Curriculum Framework: Comprehensive Consumer & Homemaking Education (Program CIP: 20.0101 - Comprehensive Consumer & Homemaking Education). Family and Consumer Sciences (Program CIP: 20.0192 - Family and Consumer Sciences)

    ERIC Educational Resources Information Center

    Arthur, Jan; Blackwell, Michelle; Clemmer, Phyllis; Cocroft, Shunda; Everett, Laurelie; Green, Coretta; West, Brenda; Yarbrough, Ruthie

    2002-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  11. 2005 Mississippi Curriculum Framework: Secondary Horticulture. (Program CIP: 01.0601 - Applied Horticulture/Horticultural Operations, General)

    ERIC Educational Resources Information Center

    Research and Curriculum Unit, 2005

    2005-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  12. 2007 Mississippi Curriculum Framework: Secondary Food Products (Meats). (Program CIP: 01.0401 - Agricultural and Food Products Processing)

    ERIC Educational Resources Information Center

    Research and Curriculum Unit, 2007

    2007-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  13. 2005 Mississippi Curriculum Framework: Secondary General Drafting. (Program CIP: 15.1301 - Drafting and Design Technology/Technician, General)

    ERIC Educational Resources Information Center

    Kirk, Karen; Ladner, Daryl; Lewis, Carroll; Moran, Sheryl; Schneider, Chester; Strickland, Ruth Ann; Welch, Amanda

    2005-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  14. 2005 Mississippi Curriculum Framework: Secondary Hotel, Restaurant, and Tourism Management. (Program CIP: 52.0901--Hospitality Administration/Management)

    ERIC Educational Resources Information Center

    Bruce, Lady Anne; Chandler, Mark; Nichols, Raynette; Nevill, Becky

    2005-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  15. 2005 Mississippi Curriculum Framework: Secondary Welding Theory and Applications. (Program CIP: 48.0508 - Welding Technology/Welder)

    ERIC Educational Resources Information Center

    Cochran, Harry; Lawrence, Kenneth; Wages, Larry; Box, Dale; Johnston, Joe; Switzer, Ronald

    2005-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and instructors are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured…

  16. 2005 Mississippi Curriculum Framework: Secondary Machine Tool Operation. (Program CIP: 48.0503 - Machine Shop Technology/Assistant)

    ERIC Educational Resources Information Center

    Gorman, Nathan; Parker, Ronald; Lurie, Charles; Maples, Thomas

    2005-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  17. 2006 Mississippi Curriculum Framework: Secondary Culinary and Related Foods Technology. (Program CIP: 20.0401 - Institutional Food Workers & Admin)

    ERIC Educational Resources Information Center

    Durand, Linda; Early, Lanell; Wood, Becky Jolly

    2006-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  18. 2007 Mississippi Curriculum Framework: Secondary Design Technology for Fashion and Interiors. (Program CIP: 19.0901 - Apparel and Textiles, General)

    ERIC Educational Resources Information Center

    Ingram, Carol; Lawrence, Angie; Pou, Margaret

    2007-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  19. 2008 Mississippi Curriculum Framework: Family and Consumer Sciences. (Program CIP: 19.9999 - Family and Consumer Sciences)

    ERIC Educational Resources Information Center

    Rosetti, Pamela; Byrd, Jenean; West, Brenda; Bigham, Melody

    2008-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  20. 2006 Mississippi Curriculum Framework: Secondary Small Engine Repair. (Program CIP: 47.0606 - Small Engine Mechanic and Repairer)

    ERIC Educational Resources Information Center

    Evans, Jimmie; Britt, Steve; Smith, Toby; Jackson, Wade

    2006-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  1. 2007 Mississippi Curriculum Framework: Secondary Custodian/Caretaker Services. (Program CIP: 19.0702 - Adult Development and Aging)

    ERIC Educational Resources Information Center

    Blake, LC; Harthcock, Sandra

    2007-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  2. 2006 Mississippi Curriculum Framework: Secondary Agricultural and Natural Resources Technology. (Program CIP: 01.0003 - Agricultural and Natural Resources)

    ERIC Educational Resources Information Center

    Bennett, Aaron; Chaney, David; Cole, Ted; Sumrall, Billy; White, Andy

    2006-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  3. 2007 Mississippi Curriculum Framework: Secondary Diesel Service Technology. (Program CIP: 47.0605 - Diesel Mechanics Technology/Technician)

    ERIC Educational Resources Information Center

    Ellison, Dave; Jackson, Edward

    2007-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  4. 2007 Mississippi Curriculum Framework: Secondary Agriculture Power and Machinery. (Program CIP: 01.0204 - Agricultural Power Machinery Operation)

    ERIC Educational Resources Information Center

    James, Lee; James, Terry; Washington, Lee; Taylor, John Grady; Rushing, Jimmy

    2007-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  5. 2006 Mississippi Curriculum Framework: Secondary Outboard Marine Engine Mechanics. (Program CIP: 47.0692 - Outboard Engine Mechanics I)

    ERIC Educational Resources Information Center

    Chavarria, Ricardo; Bounds, Terry

    2006-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  6. Mississippi Curriculum Framework for Postsecondary Funeral Services Technology Programs (Program CIP: 12.0301--Funeral Service and Mortuary Science). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the state's funeral services technology program. Presented in the introduction are a program description and suggested course sequence. Section I lists baseline competencies for the funeral…

  7. Image-Processing Program

    NASA Technical Reports Server (NTRS)

    Roth, D. J.; Hull, D. R.

    1994-01-01

    IMAGEP manipulates digital image data to effect various processing, analysis, and enhancement functions. It is keyboard-driven program organized into nine subroutines. Within subroutines are sub-subroutines also selected via keyboard. Algorithm has possible scientific, industrial, and biomedical applications in study of flows in materials, analysis of steels and ores, and pathology, respectively.

  8. "Children in Placement" (CIP): A Model for Citizen-Judicial Review.

    ERIC Educational Resources Information Center

    Wert, E. Sue; And Others

    1986-01-01

    Reports on the impact of the Children in Placement (CIP) program. CIP was found to expedite through the court the movement of children in foster care to more permanent placement. The program was used as an approach in an effort to better implement the 1980 Adoption Assistance and Child Welfare Act (P.L. 96-272). (BB)

  9. Mississippi Curriculum Framework for Jewelry and Watch Repair Cluster (Program CIP: 47.0408--Watch, Clock, and Jewelry Repair). Postsecondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for the course sequences in the jewelry design, fabrication, and repair program. Presented in the introduction are a framework of programs and courses, a program description, and suggested course sequence. Section I…

  10. Mississippi Curriculum Framework for Business and Computer Technology (Program CIP: 52.0408--Gen. Office/Clerical & Typing Service). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for secondary-level courses in business and computer technology I-II.…

  11. Mississippi Curriculum Framework for Vehicle and Mobile Equipment Mechanics (Program CIP: 47.0699--Vehicle and Mobile Equipment Mech.). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for vehicle and mobile equipment mechanics I and II. Presented first are a…

  12. Mississippi Curriculum Framework for Business and Computer Data Processing (Program CIP: 52.1201--Mgmt. Info. Systems & Business Data). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for two secondary-level courses in business and computer data processing:…

  13. Computer Program Helps Enhance Images

    NASA Technical Reports Server (NTRS)

    Stanfill, Daniel F., IV

    1994-01-01

    Pixel Pusher is Macintosh application program for viewing and performing minor enhancements on imagery. Works with color images digitized to 8 bits. Reads image files in JPL's two primary image formats VICAR and PDS as well as in Macintosh PICT format. VICAR (NPO-18076) handles array of image-processing capabilities used for variety of applications, including processing of biomedical images, cartography, imaging of Earth resources, and geological exploration. Pixel Pusher also imports color lookup tables in VICAR format for viewing images in pseudocolor (256 colors). Written in Symantec's Think C.

  14. CIP1 polypeptides and their uses

    DOEpatents

    Foreman, Pamela; Van Solingen, Pieter; Goedegebuur, Frits; Ward, Michael

    2011-04-12

    Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.

  15. ICMICs - Cancer Imaging Program

    Cancer.gov

    ICMIC grants facilitate interaction among scientists from a variety of fields to conduct multidisciplinary research on cellular and molecular imaging related to cancer. Pre-ICMIC planning grants have provided time and funds for investigators and institutions to prepare themselves, organizationally and scientifically, to establish ICMICs.

  16. Evaluation Manual for CIP Courses: Objectives and Implementation Procedures.

    ERIC Educational Resources Information Center

    Siri, Carmen

    This manual has been designed to guide courses on potato production sponsored by the International Potato Center (CIP) in Lima (Peru). It describes the CIP Course Evaluation System that is presently used and provides guidelines on how to use feedback more effectively for improving training. CIP evaluations are largely formative. The CIP focuses on…

  17. Infrared imaging spectroradiometer program overview

    NASA Astrophysics Data System (ADS)

    Rapp, Ronald J.; Register, Henry I.

    1995-06-01

    The Department of Defense, through the US Air Force's Wright Laboratory, Armament Directorate is sponsoring the development of two types of IR imaging spectroradiometers (project name: IRIS) to measure the spatial/spectral characteristics of various military targets. Design and analysis of several technical approaches were conducted during an initial phase of the program. The technical approaches investigated included: a dispersive imaging spectrometer design utilizing a fiber-optic reformatter (contractor: ERIM); an imaging acousto-optic tunable filter (AOTF) design (contractor: Westinghouse); a spatial/spectral Fourier transform infrared (FTIR) spectrometer (contractor: Bomem Inc./Canada); a spatially modulated imaging fourier transform spectrometer (contractor: Daedalus Enterprises); an imaging Fabry-Perot design (contractor: Physical Sciences Inc.). Two of these designs were selected for brass board prototype fabrication. An FTIR prototype being built by Bomem Inc., offers an instrument with high sensitivity and high spectral resolution with modest spatial performance. An imaging Fabry-Perot prototype being built by Physical Sciences Inc., offers high spatial resolution with moderate sensitivity and spectral resolution.

  18. 2005 Mississippi Curriculum Framework: Secondary Heating and Air Conditioning. (Program CIP: 47.0201 - Heating, Air Conditioning, Ventilation, and Refrigeration Maintenance Technology/Technician)

    ERIC Educational Resources Information Center

    Green, Jacob; LeBatard, Ernest; Wiggington, Donnie; Williams, Bennett

    2005-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  19. Mississippi Curriculum Framework for Family and Consumer Sciences and Related Technology (Enrichment) (CIP: 20.0101--Comprehensive Consumer & Homemkg. Ed.). Secondary Programs.

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which reflects Mississippi's statutory requirement that instructional programs be based on core curricula and performance-based assessment, contains outlines of the instructional units required in local instructional management plans and daily lesson plans for family and consumer sciences and related technology (enrichment).…

  20. 2007 Mississippi Curriculum Framework: Secondary Automotive Collision Repair Technology. (Program CIP: 47.0603 - Autobody/Collision and Repair Technology/Technician)

    ERIC Educational Resources Information Center

    Dowds, Eris; Anderson, Daniel; Sizemore, Rick; Johnson, John

    2007-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  1. 2005 Mississippi Curriculum Framework: Secondary Vehicle and Mobile Equipment Technician. (Program CIP: 47.0699 - Vehicle Maintenance and Repair Technology, Other)

    ERIC Educational Resources Information Center

    Brackeen, Scott; Freeman, Roscoe; Tiblier, Chris; Batton, James; Ealy, Houston; Simmons, Gerald

    2005-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  2. DIFMAP: an interactive program for synthesis imaging.

    NASA Astrophysics Data System (ADS)

    Shepherd, M. C.; Pearson, T. J.; Taylor, G. B.

    1994-05-01

    DIFMAP is a new interactive program for synthesis imaging. It includes data display, data editing, selfcalibration, imaging, and deconvolution. The program, written in ANSI C, runs on UNIX workstations.

  3. Complete Genome Sequence of Rahnella aquatilis CIP 78.65

    PubMed Central

    Bruce, David; Detter, Chris; Goodwin, Lynne A.; Han, James; Han, Cliff S.; Held, Brittany; Land, Miriam L.; Mikhailova, Natalia; Nolan, Matt; Pennacchio, Len; Pitluck, Sam; Tapia, Roxanne; Woyke, Tanja; Sobecky, Patricia A.

    2012-01-01

    Rahnella aquatilis CIP 78.65 is a gammaproteobacterium isolated from a drinking water source in Lille, France. Here we report the complete genome sequence of Rahnella aquatilis CIP 78.65, the type strain of R. aquatilis. PMID:22582378

  4. Complete Genome Sequence of Rahnella aquatilis CIP 78.65

    SciTech Connect

    Martinez, Robert J; Bruce, David; Detter, J C; Goodwin, Lynne A.; Han, James; Han, Cliff; Held, Brittany; Land, Miriam L; Mikhailova, Natalia; Nolan, Matt; Pennacchio, Len; Pitluck, Sam; Tapia, Roxanne; Woyke, Tanja; Sobeckya, Patricia A.

    2012-01-01

    Rahnella aquatilis CIP 78.65 is a gammaproteobacterium isolated from a drinking water source in Lille, France. Here we report the complete genome sequence of Rahnella aquatilis CIP 78.65, the type strain of R. aquatilis.

  5. Cancer Imaging

    MedlinePlus

    ... I/II Trials CIP ARRA-Funded Clinical Trials Informatics The Cancer Imaging Archive TCGA Imaging Genomics Quantitative Imaging Network LIDC-IDRI Imaging Informatics Resources News & Events News and Announcements Events – Meetings ...

  6. Career Information Partnership. CIP Status Report.

    ERIC Educational Resources Information Center

    Welton Beauchamp Professional Centre, Ottawa (Ontario).

    Employment and Immigration Canada explored, with potential partners, the feasibility of establishing a Canadian Career Information Partnership (CIP) network. A series of exploratory meetings was scheduled across Canada to determine: (1) gaps in information needed by youth and adults for effective career exploration, planning, and decision making;…

  7. Expanding Career Awareness with CIPS.

    ERIC Educational Resources Information Center

    Patterson, John

    Career Information Packets is an individually oriented career exploration program developed for high school students. The program goals are to: (1) assist students in becoming aware of their patterns, of values, interests and aptitudes in relation to the world of work; (2) to assist students in making tentative career choices; and (3) assist them…

  8. NASA/JPL's Imaging Radar Outreach Program

    NASA Technical Reports Server (NTRS)

    Freeman, A.; O'Leary, E.; Chapman, B.; Trimble, J.

    1996-01-01

    In order to build a user community for future NASA imaging radar products and programs, outreach activities have been implemented by JPL. These include: education outreach, public awareness outreach, and outreach to areas of the scientific and applications community who are not traditional imaging radar users. A key component is the NASA/JPL Imaging Radar Home Page on the World Wide Web.

  9. General-Purpose Image-Data Program

    NASA Technical Reports Server (NTRS)

    Engle, Steven W.

    1988-01-01

    Image Database computer program, IBASE, is general-purpose imagery-information system. Use accomplished either by commands or through hierarchy of menus. Analytical capabilities of IBASE include contigency tables, image filtering (low-, high-, and band-pass), proximity maps, clustering, histograms, regression, slope calculations, scaling, and Boolean manipulations. Also has interface to Cheshire Image Classification expert system. Written in FORTRAN 77.

  10. The MER/CIP Portal for Ground Operations

    NASA Technical Reports Server (NTRS)

    Chan, Louise; Desai, Sanjay; DOrtenzio, Matthew; Filman, Robtert E.; Heher, Dennis M.; Hubbard, Kim; Johan, Sandra; Keely, Leslie; Magapu, Vish; Mak, Ronald

    2003-01-01

    We developed the Mars Exploration Rover/Collaborative Information Portal (MER/CIP) to facilitate MER operations. MER/CIP provides a centralized, one-stop delivery platform integrating science and engineering data from several distributed heterogeneous data sources. Key issues for MER/CIP include: 1) Scheduling and schedule reminders; 2) Tracking the status of daily predicted outputs; 3) Finding and analyzing data products; 4) Collaboration; 5) Announcements; 6) Personalization.

  11. A comparison of current prediction imaging programs.

    PubMed

    Smith, J Dempsey; Thomas, Paul M; Proffit, William R

    2004-05-01

    To investigate perceived differences in the ability of current software to simulate the actual outcome of orthognathic surgery, we chose 10 difficult test cases with vertical discrepancies and "retreated" them using the actual surgical changes. Five programs--Dentofacial Planner Plus, Dolphin Imaging, Orthoplan, Quick Ceph Image, and Vistadent--were evaluated, by using both the default result and a refined result created with each program's enhancement tools. Three panels (orthodontists, oral-maxillofacial surgeons, and laypersons) judged the default images and the retouched simulations by ranking the simulations in side-by-side comparisons and by rating each simulation relative to the actual outcome on a 6-point scale. For the default and retouched images, Dentofacial Planner Plus was judged the best default simulation 79% and 59% of the time, respectively, and its default images received the best (lowest) mean score (2.46) on the 6-point scale. It also scored best (2.26) when the retouched images were compared, but the scores for Dolphin Imaging (2.83) and Quick Ceph (3.03) improved. Retouching had little impact on the scores for the other programs. Although the results show differences in simulation ability, selecting a software package depends on many factors. Performance and ease of use, cost, compatibility, and other features such as image and practice management tools are all important considerations. Users concerned with operating system compatibility and practice management integration might want to consider Dolphin Imaging and Quick Ceph, the programs comprising the second tier. PMID:15127020

  12. Breadboard linear array scan imager program

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The performance was evaluated of large scale integration photodiode arrays in a linear array scan imaging system breadboard for application to multispectral remote sensing of the earth's resources. Objectives, approach, implementation, and test results of the program are presented.

  13. GPU programming for biomedical imaging

    NASA Astrophysics Data System (ADS)

    Caucci, Luca; Furenlid, Lars R.

    2015-08-01

    Scientific computing is rapidly advancing due to the introduction of powerful new computing hardware, such as graphics processing units (GPUs). Affordable thanks to mass production, GPU processors enable the transition to efficient parallel computing by bringing the performance of a supercomputer to a workstation. We elaborate on some of the capabilities and benefits that GPU technology offers to the field of biomedical imaging. As practical examples, we consider a GPU algorithm for the estimation of position of interaction from photomultiplier (PMT) tube data, as well as a GPU implementation of the MLEM algorithm for iterative image reconstruction.

  14. ICMIC Institutions - Cancer Imaging Program

    Cancer.gov

    ICMIC grants facilitate interaction among scientists from a variety of fields to conduct multidisciplinary research on cellular and molecular imaging related to cancer. Pre-ICMIC planning grants have provided time and funds for investigators and institutions to prepare themselves, organizationally and scientifically, to establish ICMICs.

  15. IND Regulatory & Manufacturing Resources - Cancer Imaging Program

    Cancer.gov

    The Cancer Imaging Program has been creating Investigational New Drug Applications (IND) for imaging agents in order to engage in multi-center clinical trials of these materials. A subset of the documents filed is being made available to the research community to implement routine synthesis of tracers at their own facilities and to assist investigators with the filing of their own INDs. The first of these document sets is for F-18 fluorothymidine (FLT).

  16. A comparison of current prediction imaging programs

    PubMed Central

    Smith, J. Dempsey; Thomas, Paul M.; Proffit, William R.

    2013-01-01

    To investigate perceived differences in the ability of current software to simulate the actual outcome of orthognathic surgery, we chose 10 difficult test cases with vertical discrepancies and “retreated” them using the actual surgical changes. Five programs—Dentofacial Planner Plus, Dolphin Imaging, Orthoplan, Quick Ceph Image, and Vistadent—were evaluated, by using both the default result and a refined result created with each program’s enhancement tools. Three panels (orthodontists, oral-maxillofacial surgeons, and laypersons) judged the default images and the retouched simulations by ranking the simulations in side-by-side comparisons and by rating each simulation relative to the actual outcome on a 6-point scale. For the default and retouched images, Dentofacial Planner Plus was judged the best default simulation 79% and 59% of the time, respectively, and its default images received the best (lowest) mean score (2.46) on the 6-point scale. It also scored best (2.26) when the retouched images were compared, but the scores for Dolphin Imaging (2.83) and Quick Ceph (3.03) improved. Retouching had little impact on the scores for the other programs. Although the results show differences in simulation ability, selecting a software package depends on many factors. Performance and ease of use, cost, compatibility, and other features such as image and practice management tools are all important considerations. Users concerned with operating system compatibility and practice management integration might want to consider Dolphin Imaging and Quick Ceph, the programs comprising the second tier. PMID:15127020

  17. Cyclin kinase inhibitor p21WAF1/CIP1 in malignant melanoma: reduced expression in metastatic lesions.

    PubMed Central

    Maelandsmo, G. M.; Holm, R.; Fodstad, O.; Kerbel, R. S.; Flørenes, V. A.

    1996-01-01

    Immunohistochemical analysis of the expression of the cyclin kinase inhibitor p21WAF1/CIP1 in a panel of primary and metastatic human melanocytic tumors was performed. It was found that, independent of the p53 status, approximately 30% of the primary melanomas and 40% of the metastases completely lacked expression of this cell cycle inhibitor. Some tumors were also analyzed by Northern blotting, and in most of the cases a consistant correlation between mRNA and protein expression was observed. In four benign nevi studied, WAF1/CIP1 mRNA was expressed whereas the protein was not detected, suggesting a post-transcriptional regulation of the inhibitor in these cases. In superficial spreading melanomas, a significant correlation between protein expression and tumor thickness was found, with thin lesions showing low protein levels. Interestingly, by comparing primary and metastatic specimens obtained from the same patient, a reduction in p21WAF1/CIP1 antibody staining was observed in the latter, probably reflecting a more aggressive phenotype of the metastases. In conclusion, our results demonstrate the complexity in the relationship between p21WAF1/CIP1 expression and tumor phenotype and furthermore suggest that aberrant expression of the cyclin-dependent kinase inhibitor may be of importance in the development and progression of sporadic malignant melanoma. Images Figure 1 Figure 2 PMID:8952518

  18. Advanced imaging systems programs at DARPA MTO

    NASA Astrophysics Data System (ADS)

    Dhar, Nibir K.; Elizondo, Lee A.; Dat, Ravi; Elizondo, Shelly L.

    2013-09-01

    In this paper, we review a few selected imaging technology development programs at the Defense Advanced Research Projects Agency (DARPA) in the reflective visible to the emissive/thermal long wave infrared (LWIR) spectral bands. For the reflective visible band, results are shown for two different imagers: a gigapixel monocentric multi-scale camera design that solves the scaling issues for a high pixel count, and a wide field of view and a single photon detection camera with a large dynamic range. Also, a camera with broadband capability covering both reflective and thermal bands (0.5 μm to 5.0 μm) with >80% quantum efficiency is discussed. In the emissive/thermal band, data is presented for both uncooled and cryogenically cooled LWIR detectors with pixel pitches approaching the fundamental detection limits. By developing wafer scale manufacturing processes and reducing the pixel size of uncooled thermal imagers, it is shown that an affordable camera on a chip, capable of seeing through obscurants in day or night, is feasible. Also, the fabrication and initial performance of the world's first 5 μm pixel pitch LWIR camera is discussed. Lastly, we use an initial model to evaluate the signal to noise ratio and noise equivalent differential temperature as a function of well capacity to predict the performance for this thermal imager.

  19. AMOEBA clustering revisited. [cluster analysis, classification, and image display program

    NASA Technical Reports Server (NTRS)

    Bryant, Jack

    1990-01-01

    A description of the clustering, classification, and image display program AMOEBA is presented. Using a difficult high resolution aircraft-acquired MSS image, the steps the program takes in forming clusters are traced. A number of new features are described here for the first time. Usage of the program is discussed. The theoretical foundation (the underlying mathematical model) is briefly presented. The program can handle images of any size and dimensionality.

  20. FY 2013 Educational Facilities Master Plan and the FY 2013-2018 Capital Improvements Program

    ERIC Educational Resources Information Center

    Montgomery County Public Schools, 2012

    2012-01-01

    The FY 2013 Educational Facilities Master Plan (Master Plan) and the FY 2013-2018 Capital Improvements Program (CIP) reflect the adopted actions of the Montgomery County (Maryland) Council and integrate the facilities planning process with the annual capital budget and the six-year CIP. The CIP is developed in accordance with the Board of…

  1. FY 2011 Educational Facilities Master Plan & the FY 2011-2016 Capital Improvements Program

    ERIC Educational Resources Information Center

    Montgomery County Public Schools, 2010

    2010-01-01

    The FY 2011 Educational Facilities Master Plan (Master Plan) and FY 2011-2016 Capital Improvements Program (CIP) reflect the adopted actions of the Montgomery County (Maryland) Council and integrate the facilities planning process with the annual capital budget and the six-year CIP. The CIP is developed in accordance with the Board of Education…

  2. FY 2012 Educational Facilities Master Plan and the Amended FY 2011-2016 Capital Improvements Program

    ERIC Educational Resources Information Center

    Montgomery County Public Schools, 2011

    2011-01-01

    The FY 2012 Educational Facilities Master Plan (Master Plan) and Amendments to the FY 2011-2016 Capital Improvements Program (CIP) reflect the adopted actions of the Montgomery County (Maryland) Council and integrate the facilities planning process with the annual capital budget and the six-year CIP. The CIP is developed in accordance with the…

  3. FY 2007 Educational Facilities Master Plan and the FY 2007-2012 Capital Improvements Program

    ERIC Educational Resources Information Center

    Montgomery County Public Schools, 2006

    2006-01-01

    The FY 2007 Educational Facilities Master Plan (Master Plan) and FY 2007-2012 Capital Improvements Program (CIP) reflect the adopted actions of the Montgomery County (Maryland) Council and integrate the facilities planning process with the annual capital budget and the six-year CIP. The CIP is developed in accordance with the Board of Education…

  4. Formation of high electrical-resistivity thin surface layer on carbonyl-iron powder (CIP) and thermal stability of nanocrystalline structure and vortex magnetic structure of CIP

    NASA Astrophysics Data System (ADS)

    Sugimura, K.; Miyajima, Y.; Sonehara, M.; Sato, T.; Hayashi, F.; Zettsu, N.; Teshima, K.; Mizusaki, H.

    2016-05-01

    This study focuses on the carbonyl-iron powder (CIP) used in the metal composite bulk magnetic core for high-efficient/light-weight SiC/GaN power device MHz switching dc-dc converter, where the fine CIP with a mean diameter of 1.1 μm is used to suppress the MHz band eddy current inside the CIP body. When applying the CIP to composite core together with the resin matrix, high electrical resistivity layer must be formed on the CIP-surface in order to suppress the overlapped eddy current between adjacent CIPs. In this study, tens nm thick silica (SiO2) was successfully deposited on the CIP-surface by using hydrolysis of TEOS (Si(OC2H5)4). Also tens nm thick oxidized layer of the CIP-surface was successfully formed by using CIP annealing in dry air. The SiC/GaN power device can operate at ambient temperature over 200 degree-C, and the composite magnetic core is required to operate at such ambient temperature. The as-made CIP had small coercivity below 800 A/m (10 Oe) due to its nanocrystalline-structure and had a single vortex magnetic structure. From the experimental results, both nanocrystalline and single vortex magnetic structure were maintained after heat-exposure of 250 degree-C, and the powder coercivity after same heat-exposure was nearly same as that of the as-made CIP. Therefore, the CIP with thermally stable nanocrystalline-structure and vortex magnetic state was considered to be heat-resistant magnetic powder used in the iron-based composite core for SiC/GaN power electronics.

  5. CIP2A Promotes T-Cell Activation and Immune Response to Listeria monocytogenes Infection.

    PubMed

    Côme, Christophe; Cvrljevic, Anna; Khan, Mohd Moin; Treise, Irina; Adler, Thure; Aguilar-Pimentel, Juan Antonio; Au-Yeung, Byron; Sittig, Eleonora; Laajala, Teemu Daniel; Chen, Yiling; Oeder, Sebastian; Calzada-Wack, Julia; Horsch, Marion; Aittokallio, Tero; Busch, Dirk H; Ollert, Markus W; Neff, Frauke; Beckers, Johannes; Gailus-Durner, Valerie; Fuchs, Helmut; Hrabě de Angelis, Martin; Chen, Zhi; Lahesmaa, Riitta; Westermarck, Jukka

    2016-01-01

    The oncoprotein Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A) is overexpressed in most malignancies and is an obvious candidate target protein for future cancer therapies. However, the physiological importance of CIP2A-mediated PP2A inhibition is largely unknown. As PP2A regulates immune responses, we investigated the role of CIP2A in normal immune system development and during immune response in vivo. We show that CIP2A-deficient mice (CIP2AHOZ) present a normal immune system development and function in unchallenged conditions. However when challenged with Listeria monocytogenes, CIP2AHOZ mice display an impaired adaptive immune response that is combined with decreased frequency of both CD4+ T-cells and CD8+ effector T-cells. Importantly, the cell autonomous effect of CIP2A deficiency for T-cell activation was confirmed. Induction of CIP2A expression during T-cell activation was dependent on Zap70 activity. Thus, we reveal CIP2A as a hitherto unrecognized mediator of T-cell activation during adaptive immune response. These results also reveal CIP2AHOZ as a possible novel mouse model for studying the role of PP2A activity in immune regulation. On the other hand, the results also indicate that CIP2A targeting cancer therapies would not cause serious immunological side-effects. PMID:27100879

  6. CIP2A Promotes T-Cell Activation and Immune Response to Listeria monocytogenes Infection

    PubMed Central

    Cvrljevic, Anna; Khan, Mohd Moin; Treise, Irina; Adler, Thure; Aguilar-Pimentel, Juan Antonio; Au-Yeung, Byron; Sittig, Eleonora; Laajala, Teemu Daniel; Chen, Yiling; Oeder, Sebastian; Calzada-Wack, Julia; Horsch, Marion; Aittokallio, Tero; Busch, Dirk H.; Ollert, Markus W.; Neff, Frauke; Beckers, Johannes; Gailus-Durner, Valerie; Fuchs, Helmut; de Angelis, Martin Hrabě; Chen, Zhi; Lahesmaa, Riitta; Westermarck, Jukka

    2016-01-01

    The oncoprotein Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A) is overexpressed in most malignancies and is an obvious candidate target protein for future cancer therapies. However, the physiological importance of CIP2A-mediated PP2A inhibition is largely unknown. As PP2A regulates immune responses, we investigated the role of CIP2A in normal immune system development and during immune response in vivo. We show that CIP2A-deficient mice (CIP2AHOZ) present a normal immune system development and function in unchallenged conditions. However when challenged with Listeria monocytogenes, CIP2AHOZ mice display an impaired adaptive immune response that is combined with decreased frequency of both CD4+ T-cells and CD8+ effector T-cells. Importantly, the cell autonomous effect of CIP2A deficiency for T-cell activation was confirmed. Induction of CIP2A expression during T-cell activation was dependent on Zap70 activity. Thus, we reveal CIP2A as a hitherto unrecognized mediator of T-cell activation during adaptive immune response. These results also reveal CIP2AHOZ as a possible novel mouse model for studying the role of PP2A activity in immune regulation. On the other hand, the results also indicate that CIP2A targeting cancer therapies would not cause serious immunological side-effects. PMID:27100879

  7. Development of mice without Cip/Kip CDK inhibitors

    SciTech Connect

    Tateishi, Yuki; Matsumoto, Akinobu; Kanie, Tomoharu; Hara, Eiji; Nakayama, Keiko; Nakayama, Keiichi I.

    2012-10-19

    Highlights: Black-Right-Pointing-Pointer Mice lacking Cip/Kip CKIs (p21, p27, and p57) survive until embryonic day 13.5. Black-Right-Pointing-Pointer Proliferation of MEFs lacking all three Cip/Kip CKIs appears unexpectedly normal. Black-Right-Pointing-Pointer CDK2 kinase activity of the triple mutant MEFs is increased in G0 phase. -- Abstract: Timely exit of cells from the cell cycle is essential for proper cell differentiation during embryogenesis. Cyclin-dependent kinase (CDK) inhibitors (CKIs) of the Cip/Kip family (p21, p27, and p57) are negative regulators of cell cycle progression and are thought to be essential for development. However, the extent of functional redundancy among Cip/Kip family members has remained largely unknown. We have now generated mice that lack all three Cip/Kip CKIs (TKO mice) and compared them with those lacking each possible pair of these proteins (DKO mice). We found that the TKO embryos develop normally until midgestation but die around embryonic day (E) 13.5, slightly earlier than p27/p57 DKO embryos. The TKO embryos manifested morphological abnormalities as well as increased rates of cell proliferation and apoptosis in the placenta and lens that were essentially indistinguishable from those of p27/p57 DKO mice. Unexpectedly, the proliferation rate and cell cycle profile of mouse embryonic fibroblasts (MEFs) lacking all three Cip/Kip CKIs did not differ substantially from those of control MEFs. The abundance and kinase activity of CDK2 were markedly increased, whereas CDK4 activity and cyclin D1 abundance were decreased, in both p27/p57 DKO and TKO MEFs during progression from G{sub 0} to S phase compared with those in control MEFs. The extents of the increase in CDK2 activity and the decrease in CDK4 activity and cyclin D1 abundance were greater in TKO MEFs than in p27/p57 DKO MEFs. These results suggest that p27 and p57 play an essential role in mouse development after midgestation, and that p21 plays only an auxiliary role in

  8. Radiology Network (ACRIN) - Cancer Imaging Program

    Cancer.gov

    ACRIN is funded to improve the quality and utility of imaging in cancer research and cancer care through expert, multi-institutional clinical evaluation of discoveries and technological innovations relevant to imaging science as applied in clinical oncology.

  9. Cellular localization of CIP2A determines its prognostic impact in superficial spreading and nodular melanoma.

    PubMed

    Flørenes, Vivi Ann; Emilsen, Elisabeth; Dong, Hiep Phuc; Førsund, Mette; Holm, Ruth; Slipicevic, Ana

    2015-06-01

    Cancerous inhibitor of protein phosphatase 2A (CIP2A) is an important oncogene contributing to cancer progression partially by regulating cMYC and AKT. We examined CIP2A expression in cutaneous melanomas, its association with clinicopathological parameters and mapped molecular mechanisms regulated by CIP2A in vitro. CIP2A expression was analyzed by immunohistochemistry in 17 nevi, 132 primary melanomas and 49 metastases. Effects of siRNA-mediated down-regulation on proliferation, apoptosis and signaling pathways were assessed in melanoma cell lines. In superficial spreading melanomas (SSM), high nuclear CIP2A expression was associated with poor overall survival (OS) (P = 0.0018). Surprisingly, high cytoplasmic expression was related to improved relapse-free (P = 0.031) and OS (P = 0.014) in nodular melanomas (NM). In vitro experiments revealed that CIP2A can regulate proliferation and/or apoptosis partially through the PI3K/AKT pathway but also independently. In summary, CIP2A could represent a potential therapeutic target in SSM. However, in NM cytoplasmic CIP2A is associated with improved prognosis indicating that CIP2A has distinct, complex functions dependent on the molecular context and histological subtype. As seen in other cancer types, CIP2A can influence cMYC and AKT, but our data also suggest that in melanoma it has additional targets which need to be identified. PMID:25663244

  10. The anchorage function of CipA (CelL), a scaffolding protein of the Clostridium thermocellum cellulosome.

    PubMed Central

    Kruus, K; Lua, A C; Demain, A L; Wu, J H

    1995-01-01

    Enzymatic cellulose degradation is a heterogeneous reaction requiring binding of soluble cellulase molecules to the solid substrate. Based on our studies of the cellulase complex of Clostridium thermocellum (the cellulosome), we have previously proposed that such binding can be brought about by a special "anchorage subunit." In this "anchor-enzyme" model, CipA (a major subunit of the cellulosome) enhances the activity of CelS (the most abundant catalytic subunit of the cellulosome) by anchoring it to the cellulose surface. We have subsequently reported that CelS contains a conserved duplicated sequence at its C terminus and that CipA contains nine repeated sequences with a cellulose binding domain (CBD) in between the second and third repeats. In this work, we reexamined the anchor-enzyme mechanism by using recombinant CelS (rCelS) and various CipA domains, CBD, R3 (the repeat next to CBD), and CBD/R3, expressed in Escherichia coli. As analyzed by non-denaturing gel electrophoresis, rCelS, through its conserved duplicated sequence, formed a stable complex with R3 or CBD/R3 but not with CBD. Although R3 or CBD alone did not affect the binding of rCelS to cellulose, such binding was dependent on CBD/R3, indicating the anchorage role of CBD/R3. Such anchorage apparently increased the rCelS activity toward crystalline cellulose. These results substantiate the proposed anchor-enzyme model and the expected roles of individual CipA domains and the conserved duplicated sequence of CelS. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 PMID:7568112

  11. Human Genome Program Image Gallery (from genomics.energy.gov)

    DOE Data Explorer

    This collection contains approximately 240 images from the genome programs of DOE's Office of Science. The images are divided into galleries related to biofuels research, systems biology, and basic genomics. Each image has a title, a basic citation, and a credit or source. Most of the images are original graphics created by the Genome Management Information System (GMIS). GMIS images are recognizable by their credit line. Permission to use these graphics is not needed, but please credit the U.S. Department of Energy Genome Programs and provide the website http://genomics.energy.gov. Other images were provided by third parties and not created by the U.S. Department of Energy. Users must contact the person listed in the credit line before using those images. The high-resolution images can be downloaded.

  12. Examining the Perceptions of Brand Images Regarding Competing MBA Programs

    ERIC Educational Resources Information Center

    Hinds, Timothee; Falgoust, Dexter; Thomas, Kerry, Jr.; Budden, Michael C.

    2010-01-01

    In today's economic environment, it is crucial to create a strong, consistent brand image within a graduate business program. This study examines the perceptions that students at Southeastern Louisiana University hold about its MBA program and the MBA programs of its main competitors. A focus group was conducted to identify competitors and factors…

  13. Laboratory evaluation of the CIP 10 personal dust sampler.

    PubMed

    Gero, A; Tomb, T

    1988-06-01

    The "capteur individuel de poussiere" CIP 10 personal dust sampler--developed by the Centre d'Etudes et Recherches de Charbonnages de France (CERCHAR) research organization--is a small, quiet, lightweight unit which samples at a flow rate of 10 L/min. It is a three-stage sampler, using two stages to remove nonrespirable dust particles and one stage to collect the respirable fraction. Airflow through the sampler is induced by the third stage, which is a rotating collector cup that contains a fine grade sponge. Laboratory tests were conducted in a dust chamber using aerosols of Arizona road dust, coal dust and silica dust. Aerosol concentrations measured with the CIP 10 were compared to those measured with the coal mine dust personal sampler unit used in the United States. The results of this study showed that aerosol concentrations measured with the CIP 10 were linearly related to those obtained with the coal mine dust personal sampler. The relationship, however, was dependent on preselector configuration and aerosol characteristics. The collection medium allows some small particles (less than 3 microns) to pass through the sampler without being collected. As much as 13% (by weight) of the aerosol that penetrated through the preseparating stages was exhausted from the sampler. PMID:2840817

  14. A Macintosh and Windows program for assessing body-image disturbance using adjustable image distortion.

    PubMed

    Shibata, Seiji

    2002-02-01

    Body image is one of the most important concepts in the study of eating disorders. The assessment and treatment of body-image issues are considered to be integral aspects of assessment and clinical management of eating disorders (Thompson, 1996b). The program, BodyImage, is software for the assessment of body-image disturbance. It uses an image-distorting technique to estimate body size. The image of the whole body or parts of the body can be captured as a digital image by a digital camera. Response data from participants are recorded as ASCII files so that other computer programs such as spreadsheets or word processing programs can handle the data. BodyImage works on personal computers, both Macintosh and Windows. It is available at no cost, and it can be obtained from the following URL: http://homepage2.nifty.com/s_shibata/softwares/bodyimage.html. PMID:12060995

  15. MDM2 promotes p21waf1/cip1 proteasomal turnover independently of ubiquitylation.

    PubMed

    Jin, Yetao; Lee, Hunjoo; Zeng, Shelya X; Dai, Mu-Shui; Lu, Hua

    2003-12-01

    The CDK inhibitor p21waf1/cip1 is degraded by a ubiquitin-independent proteolytic pathway. Here, we show that MDM2 mediates this degradation process. Overexpression of wild-type or ring finger-deleted, but not nuclear localization signal (NLS)-deleted, MDM2 decreased p21waf1/cip1 levels without ubiquitylating this protein and affecting its mRNA level in p53(-/-) cells. This decrease was reversed by the proteasome inhibitors MG132 and lactacystin, by p19(arf), and by small interfering RNA (siRNA) against MDM2. p21waf1/cip1 bound to MDM2 in vitro and in cells. The p21waf1/cip1-binding-defective mutant of MDM2 was unable to degrade p21waf1/cip1. MDM2 shortened the half-life of both exogenous and endogenous p21waf1/cip1 by 50% and led to the degradation of its lysine-free mutant. Consequently, MDM2 suppressed p21waf1/cip1-induced cell growth arrest of human p53(-/-) and p53(-/-)/Rb(-/-)cells. These results demonstrate that MDM2 directly inhibits p21waf1/cip1 function by reducing p21waf1/cip1 stability in a ubiquitin-independent fashion. PMID:14633995

  16. CIP2A down regulation enhances the sensitivity of pancreatic cancer cells to gemcitabine

    PubMed Central

    He, Jin; Zhao, Long; Wang, Xiaodong; Li, Zhennan; Qian, Jianjun

    2016-01-01

    Cancerous inhibitor of protein phosphatase 2A (CIP2A) is an oncoprotein which participates in inhibiting tumor apoptosis in pancreatic cancer cells. Using immunohistochemical staining, we investigated the expression of CIP2A protein in 72 cases of human pancreatic ductal adenocarcinoma (PDAC) tissue and 27 cases of adjacent normal pancreatic tissue. The positive rate of CIP2A protein expression in pancreatic cancer tissue was70.83 %, which was significantly higher than that in adjacent non- cancerous pancreatic tissue (11.11%). The expression of CIP2A was found to be correlated with TNM stage, but not correlated with age, gender, tumor location, smoking status, alcohol consumption, diabetes, high blood pressure, BMI, tumor size, lymph node metastasis or distant metastases. Kaplan- Meier survival analysis showed that patients with positive CIP2A protein expression had a lower overall survival rate than patients without CIP2A expression. COX regression analysis indicated that expression of CIP2A was an independent prognostic factor for pancreatic ductal adenocarcinoma. In addition, down-regulation of CIP2A inhibited cell proliferation and increased sensitivity to gemcitabine in pancreatic cancer cells by decreasing AKT signaling pathway. Our results indicated that down-regulation of CIP2A could be a novel therapeutic strategy for pancreatic cancer. PMID:26894380

  17. MYC accelerates p21CIP-induced megakaryocytic differentiation involving early mitosis arrest in leukemia cells.

    PubMed

    Muñoz-Alonso, María J; Ceballos, Laura; Bretones, Gabriel; Frade, Pilar; León, Javier; Gandarillas, Alberto

    2012-05-01

    p21(CIP) is a potent cell cycle inhibitor often up-regulated in differentiation. Protooncogene MYC induces cell growth and proliferation, inhibits differentiation and represses p21(CIP). However, both molecules are involved in processes of polyploidisation, cell size increase, differentiation and senescence. It is unclear why MYC has a dual role in differentiation. We have previously shown that overexpression of p21(CIP) in K562 myeloid cells induces megakaryocytic differentiation with polyploidy. We have now investigated the requirements for p21(CIP) to block mitosis and induce differentiation in the presence of overactivated MYC. Silencing and over-expression studies showed that p21(CIP) is required to induce differentiation. However, the expression of p21(CIP) needs to be transient to irreversibly inhibit mitosis but not DNA replication, what leads to polyploidy. Transient overexpression of p21(CIP) caused early down-regulation of mitotic Cyclins and up-regulation of G1/S Cyclins D and E, changes typical of endoreplication. Interestingly, over-activation of MYC did not release the proliferative block imposed by p21(CIP) and instead, accelerated cell size increase, megakaryocytic differentiation and polyploidisation. Our data suggests that in some systems p21(CIP) takes part in a mitosis control driving MYC-induced cellular growth into differentiation. PMID:21769863

  18. Ultrasound Research Interface - Cancer Imaging Program

    Cancer.gov

    The ultrasound research interface permits extensive instrument parameter control of a commercially available scanner that allows access to, and export of, the beam-formed signal data while simultaneously displaying the ultrasound system-processed data as a clinical image.

  19. Program For Thresholding In Digital Images

    NASA Technical Reports Server (NTRS)

    Nolf, Scott R.; Avis, Elizabeth L.; Matthews, Christine G.; Stacy, Kathryn

    1994-01-01

    THRTOOL program applies thresholding techniques to Sun rasterfiles. Provides for choice among four methods of thresholding. Written in C language and implemented on Sun series and Silicon Graphics IRIS machines.

  20. Program Generates Images Of Solid Surfaces

    NASA Technical Reports Server (NTRS)

    Goza, Sharon P.; Bell, Bradley; Shores, David

    1991-01-01

    Solid Surface Modeler (SSM) computer program generates three-dimensional computer models of solid surfaces for use in visual analysis and animation. Provides advanced functions, including Constructive Solid Geometry, Skin Construction, Tube Construction, Material Property Editing, and Texture Map Tools. Allows user to change such attributes as color, reflectivity, smoothing, and position of object easily. Model saved in ASCII or binary format for use in another program or saved in edit format for reloading in SSM. Written in standard C.

  1. CIP2A expression and prognostic role in patients with esophageal adenocarcinoma.

    PubMed

    Rantanen, Tuomo; Kauttu, Tuuli; Åkerla, Jonne; Honkanen, Teemu; Krogerus, Leena; Salo, Jarmo; Paavonen, Timo; Oksala, Niku

    2013-01-01

    CIP2A is overexpressed in many cancers, including esophageal squamous cell carcinoma. The regulation of c-MYC and CIP2A expression is characterized by a positive feedback mechanism facilitating the expression of both of them and accelerating cancer cell proliferation in gastric cancer. Increased CIP2A expression is a predictor of poor survival in some cancers. The incidence of positive CIP2A immunostaining and its association with c-MYC and its predictive value in esophageal adenocarcinoma are unknown. All esophageal adenocarcinoma patients from 1990 to 2007 with sufficient material for analysis of CIP2A and c-MYC in two university hospitals were included in the study. In addition, biopsies from Barrett's epithelium from the cancer patients and control tissue from normal esophageal mucosa adjacent to the tumor were included. CIP2A was moderately or strongly positive in 77.9 %, and c-MYC in 93.8 % of the cancer specimens. These frequencies were statistically different from the expression in normal esophageal epithelium. In addition, there was a positive correlation between CIP2A and c-MYC expression (p = 0.018). According to adjusted Cox regression survival analysis, CIP2A and c-MYC had no effect on survival. However, among patients with stage IVA-IVB cancer, there was a trend toward poor prognosis in CIP2A-positive patients. The expression of CIP2A and c-MYC was associated with each other, and their overexpression was found in most cases of esophageal adenocarcinoma. However, CIP2A and c-MYC had no effect on survival. PMID:23925667

  2. Carbon turnover in pore spaces - CIPS model approach

    NASA Astrophysics Data System (ADS)

    Kuka, Katrin

    2010-05-01

    The CIPS (Carbon turnover In Pore Spaces) model has been developed to overcome the constraints of conceptual pools and to get a better insight into the nature of carbon stabilization in soil (KUKA, 2007). This pure carbon turnover model was implemented in CANDY (CArbon and Nitrogen Dynamics) model system (Franko, 1995). The CIPS model did overcome the empirical pools taking into account soil structure effects. It is based on quality driven primary stabilisation mechanism (recalcitrance of SOM) and process driven secondary stabilisation mechanism (place of turnover) of SOM in soil. In addition to the division of SOM in the qualitative pools on the basis of chemical measurability, a dependence of the turnover conditions from the location of SOM in pore space is implemented taking into account different turnover conditions in the particular pore space and the accessibility for microbial biomass. The main assumption of the CIPS model is that the biological activity is not evenly distributed through the whole pore space. The pore space classes - micro, meso and macro pores - used in the model are marked by wilting point, field capacity and pore volume as a first approach. Because of the poor aeration in the micro pores they show very low biological activity leading to a strong protection of the carbon localized in this pore space. The biological active time (BAT) concept of the CANDY model was adapted to the CIPS model in order to calculate the distribution of biological activity for each pore space class. The reduction functions of the turnover active time concept of CANDY model, related to soil temperature, soil moisture, soil texture, relative air volume and distance to the soil surface are multiplied by the step width of calendar time producing the transformed time step as total BAT (BATtot). The calculated BATtot corresponds to the time that would be required under optimal conditions in the laboratory to perform the same turnover result as under the given

  3. Network for Translational Research - Cancer Imaging Program

    Cancer.gov

    Cooperative agreement (U54) awards to establish Specialized Research Resource Centers that will participate as members of a network of inter-disciplinary, inter-institutional research teams for the purpose of supporting translational research in optical imaging and/or spectroscopy in vivo, with an emphasis on multiple modalities.

  4. An image-processing program for automated counting

    USGS Publications Warehouse

    Cunningham, D.J.; Anderson, W.H.; Anthony, R.M.

    1996-01-01

    An image-processing program developed by the National Institute of Health, IMAGE, was modified in a cooperative project between remote sensing specialists at the Ohio State University Center for Mapping and scientists at the Alaska Science Center to facilitate estimating numbers of black brant (Branta bernicla nigricans) in flocks at Izembek National Wildlife Refuge. The modified program, DUCK HUNT, runs on Apple computers. Modifications provide users with a pull down menu that optimizes image quality; identifies objects of interest (e.g., brant) by spectral, morphometric, and spatial parameters defined interactively by users; counts and labels objects of interest; and produces summary tables. Images from digitized photography, videography, and high- resolution digital photography have been used with this program to count various species of waterfowl.

  5. Coordinated Investigation Programs: a status report

    NASA Astrophysics Data System (ADS)

    Stamper, Richard

    The building blocks of the IHY science program are the CIPs - the Coordinated Investigation Programs. Following a "bottom-up" philosophy, these are essentially autonomous investigations proposed and executed by self-selected groups of scientists that span the range of IHY science. Naturally, there are points of contact and overlap between the subjects of study of the various CIPS. To permit an easier appreciation of the scope of the program and of the connections between CIPs, and also to promote collaboration between them, the CIPs have therefore been grouped into seven "Disciplines": Solar; Heliospheric/Cosmic Rays; Magnetospheres; Ionized atmospheres; Neutral atmospheres; Climate; Meteors/Meteoroids/Interstellar Dust. A report will be given on the status of the CIPs and on the effectiveness of the mechanisms established for the IHY to support them individually and to build connections between them.

  6. CIP2A regulates cancer metabolism and CREB phosphorylation in non-small cell lung cancer.

    PubMed

    Peng, Bo; Lei, Ningjing; Chai, Yurong; Chan, Edward K L; Zhang, Jian-Ying

    2015-01-01

    The cancerous inhibitor of protein phosphatase 2A (CIP2A) is a recently characterized endogenous inhibitor of the phosphatase activity of protein phosphatase 2A (PP2A), which extends the half-life of oncogenic protein c-myc and promotes in vivo tumor growth. The function of CIP2A in cancer progression is still poorly understood. To uncover the underlying mechanism of CIP2A-mediated cell proliferation, we implemented a two-dimensional electrophoresis (2DE)-based proteomic approach to examine lung cancer cell H1299 with and without CIP2A. We found 47 proteins differentially expressed where 19 proteins were upregulated and 28 proteins were downregulated. These were categorized into functional groups such as metabolism (25%), transcriptional and translational control (23%), and the signaling pathway and protein degradation (20%). On one hand, we validate our proteomic work by measuring the metabolic change. The knockdown of CIP2A decreased the expression of LDH-A as well as the enzymatic activity, accompanying with a decreased lactate production, an increased NADH/NAD+ ratio and ROS production. On the other hand, we found that CIP2A may regulate CREB activity through bioinformatics analysis. Our following experiments showed that, CIP2A positively regulated the phosphorylation of CREB in response to the serum treatment. Therefore, our proteomic study suggested that CIP2A mediates cancer progression through the metabolic pathway and intracellular signaling cascade. PMID:25325377

  7. CIP Training Manual: Collaborative Information Portal Advance Training Information for Field Test Participants

    NASA Technical Reports Server (NTRS)

    Schreiner, John; Clancy, Daniel (Technical Monitor)

    2002-01-01

    The Collaborative Information Portal (CIP) is a web-based information management and retrieval system. Its purpose is to provide users at MER (Mars Exploration Rover) mission operations with easy access to a broad range of mission data and products and contextual information such as the current operations schedule. The CIP web-server provides this content in a user customizable web-portal environment. Since CIP is still under development, only a subset of the full feature set will be available for the EDO field test. The CIP web-portal will be accessed through a standard web browser. CIP is intended to be intuitive and simple to use, however, at the training session, users will receive a one to two page reference guide, which should aid them in using CIP. Users must provide their own computers for accessing CIP during the field test. These computers should be configured with Java 1.3 and a Java 2 enabled browser. Macintosh computers should be running OS 10.1.3 or later. Classic Mac OS (OS 9) is not supported. For more information please read section 7.3 in the FIASCO Rover Science Operations Test Mission Plan. Several screen shots of the Beta Release of CIP are shown on the following pages.

  8. Implementing a breast MR imaging program: all things considered.

    PubMed

    Raza, Sughra

    2010-05-01

    The role of magnetic resonance (MR) imaging in breast imaging and evaluation has increased rapidly. MR imaging now encompasses diagnostic evaluation as well as screening for breast cancer in high-risk groups, monitoring the extent of disease and the response to chemotherapy. It is expected that the utility of breast MR imaging will continue to increase, requiring additional facilities and expertise. Establishing a breast MR imaging program requires familiarity with several unique issues pertaining to the nature of this imaging modality. This article attempts to address some of these issues, including selection of a magnet based on needs of the particular practice and magnet field strength, selection of a dedicated breast coil, magnet location and siting, advantages and challenges of higher strength magnets such as 3 Tesla, establishing a referral base, scheduling of breast MR examinations, patient safety concerns, and examination interpretation and reporting. PMID:20494305

  9. High efficiency CIP 10-I personal inhalable aerosol sampler

    NASA Astrophysics Data System (ADS)

    Görner, P.; Wrobel, R.; Simon, X.

    2009-02-01

    The CIP 10 personal aerosol sampler was first developed by Courbon for sampling the respirable fraction of mining dust. This respirable aerosol sampler was further improved by Fabries, then selectors for sampling thoracic and inhalable aerosols were designed. Kenny et al. evaluated the particle-size dependent sampling efficiency of the inhalable version in a large-scale wind tunnel using a life-size dummy. The authors found that the overall sampling efficiency decreases more rapidly than the CEN-ISO-ACGIH target efficiency curve. Görner and Witschger measured the aspiration efficiency of the CIP 10 omni-directional inlet. They found that the aspiration efficiency was high enough for inhalable aerosol sampling. This result led to the conclusion that the low sampling efficiency is due to some internal losses of the aspirated particles before they reach the final sampling stage, namely the CIP 10 rotating filter. Based on the assumption that the inhalable particles are selected at selector aspiration level, an experimental research project was conducted to improve particle transmission to the collection stage of the sampler. Two different inhalable selectors were designed by Görner and tested in a laboratory wind tunnel. The transmission efficiency of both models was measured by Roger following an experimental protocol described by Witschger. The T-shaped air flow circuit was finally adopted to draw the aspirated particles into the final collection stage of the CIP 10. Actually, in this selector, the almost horizontally aspirated particles should be conducted vertically to the rotating cup. In two previous prototypes, particles could be deposited in certain places by inertia (where the aerosol was forced to deviate drastically) or by sedimentation (where the aerosol decelerated). The aerodynamic behaviour of the adopted solution causes the particles to accelerate radially between two horizontal plates before they enter a vertical tube. This acceleration avoids the

  10. FRAPTRAN Predictability of High Burnup Advanced Fuel Performance: Analysis of the CABRI CIP0-1 and CIP0-2 Experiments

    SciTech Connect

    Del Barrio, M.T.; Herranz, L.E.

    2007-07-01

    Adequacy of analytical tools to estimate advanced high burnup fuel during a power pulse need to be soundly proven. Most of models in codes dealing with transient are extrapolations of those developed for lower irradiations. In addition, lack of open information prevents often a proper account of mechanical properties of new advanced cladding material. These circumstances make experimental programs on high burnup fuel performance an indispensable tool to enhance safety codes predictability through building up sound databases on which models can be extended or developed and on which suitable code performance can be proven. The experiments CIP0-1 and CIP0-2, carried out on 2002 in the CABRI reactor, can be seen as reference tests to investigate high burnup fuel response to RIA transients. Fuel rods of up to 75 GWd/tU (average rod burnup) encapsulated in advanced cladding materials (ZIRLO and M5) were submitted to power pulses of about 30 ms of half maximum width that injected 90-100 cal/g after 1.2 s. None of the rodlets failed during the experiments, but they underwent deformation that was experimentally determined. The FRAPTRAN code has been used for the analysis of these RIA tests. The fuel rod characterization necessary for FRAPTRAN at the end of the base irradiation, prior to the transient, was provided by FRAPCON-3. An investigation of major deviations of fuel rod characterization at the end of the base irradiation has highlighted that thermal uncertainties could result in outstanding discrepancies in FGR estimates. Transient comparison with the available data shows that FRAPTRAN presents a relatively good agreement in permanent clad hoop strain and overestimates significantly the axial elongation of the cladding. The potential effect of approximations made in describing the cladding mechanical behavior, the fuel-to-clad relative movement and the pre-transient gap width, have been all discussed. Given existing uncertainties, a conclusive statement could not be

  11. A new programming metaphor for image processing procedures

    NASA Technical Reports Server (NTRS)

    Smirnov, O. M.; Piskunov, N. E.

    1992-01-01

    Most image processing systems, besides an Application Program Interface (API) which lets users write their own image processing programs, also feature a higher level of programmability. Traditionally, this is a command or macro language, which can be used to build large procedures (scripts) out of simple programs or commands. This approach, a legacy of the teletypewriter has serious drawbacks. A command language is clumsy when (and if! it attempts to utilize the capabilities of a multitasking or multiprocessor environment, it is but adequate for real-time data acquisition and processing, it has a fairly steep learning curve, and the user interface is very inefficient,. especially when compared to a graphical user interface (GUI) that systems running under Xll or Windows should otherwise be able to provide. ll these difficulties stem from one basic problem: a command language is not a natural metaphor for an image processing procedure. A more natural metaphor - an image processing factory is described in detail. A factory is a set of programs (applications) that execute separate operations on images, connected by pipes that carry data (images and parameters) between them. The programs function concurrently, processing images as they arrive along pipes, and querying the user for whatever other input they need. From the user's point of view, programming (constructing) factories is a lot like playing with LEGO blocks - much more intuitive than writing scripts. Focus is on some of the difficulties of implementing factory support, most notably the design of an appropriate API. It also shows that factories retain all the functionality of a command language (including loops and conditional branches), while suffering from none of the drawbacks outlined above. Other benefits of factory programming include self-tuning factories and the process of encapsulation, which lets a factory take the shape of a standard application both from the system and the user's point of view, and

  12. The vectorization of a ray tracing program for image generation

    NASA Technical Reports Server (NTRS)

    Plunkett, D. J.; Cychosz, J. M.; Bailey, M. J.

    1984-01-01

    Ray tracing is a widely used method for producing realistic computer generated images. Ray tracing involves firing an imaginary ray from a view point, through a point on an image plane, into a three dimensional scene. The intersections of the ray with the objects in the scene determines what is visible at the point on the image plane. This process must be repeated many times, once for each point (commonly called a pixel) in the image plane. A typical image contains more than a million pixels making this process computationally expensive. A traditional ray tracing program processes one ray at a time. In such a serial approach, as much as ninety percent of the execution time is spent computing the intersection of a ray with the surface in the scene. With the CYBER 205, many rays can be intersected with all the bodies im the scene with a single series of vector operations. Vectorization of this intersection process results in large decreases in computation time. The CADLAB's interest in ray tracing stems from the need to produce realistic images of mechanical parts. A high quality image of a part during the design process can increase the productivity of the designer by helping him visualize the results of his work. To be useful in the design process, these images must be produced in a reasonable amount of time. This discussion will explain how the ray tracing process was vectorized and gives examples of the images obtained.

  13. CIP2A oncoprotein controls cell growth and autophagy through mTORC1 activation

    PubMed Central

    Puustinen, Pietri; Rytter, Anna; Mortensen, Monika; Kohonen, Pekka; Moreira, José M.

    2014-01-01

    mTORC1 (mammalian target of rapamycin complex 1) integrates information regarding availability of nutrients and energy to coordinate protein synthesis and autophagy. Using ribonucleic acid interference screens for autophagy-regulating phosphatases in human breast cancer cells, we identify CIP2A (cancerous inhibitor of PP2A [protein phosphatase 2A]) as a key modulator of mTORC1 and autophagy. CIP2A associates with mTORC1 and acts as an allosteric inhibitor of mTORC1-associated PP2A, thereby enhancing mTORC1-dependent growth signaling and inhibiting autophagy. This regulatory circuit is reversed by ubiquitination and p62/SQSTM1-dependent autophagic degradation of CIP2A and subsequent inhibition of mTORC1 activity. Consistent with CIP2A’s reported ability to protect c-Myc against proteasome-mediated degradation, autophagic degradation of CIP2A upon mTORC1 inhibition leads to destabilization of c-Myc. These data characterize CIP2A as a distinct regulator of mTORC1 and reveals mTORC1-dependent control of CIP2A degradation as a mechanism that links mTORC1 activity with c-Myc stability to coordinate cellular metabolism, growth, and proliferation. PMID:24590173

  14. CIP2A is a candidate therapeutic target in clinically challenging prostate cancer cell populations.

    PubMed

    Khanna, Anchit; Rane, Jayant K; Kivinummi, Kati K; Urbanucci, Alfonso; Helenius, Merja A; Tolonen, Teemu T; Saramäki, Outi R; Latonen, Leena; Manni, Visa; Pimanda, John E; Maitland, Norman J; Westermarck, Jukka; Visakorpi, Tapio

    2015-08-14

    Residual androgen receptor (AR)-signaling and presence of cancer stem-like cells (SCs) are the two emerging paradigms for clinically challenging castration-resistant prostate cancer (CRPC). Therefore, identification of AR-target proteins that are also overexpressed in the cancer SC population would be an attractive therapeutic approach.Our analysis of over three hundred clinical samples and patient-derived prostate epithelial cultures (PPECs), revealed Cancerous inhibitor of protein phosphatase 2A (CIP2A) as one such target. CIP2A is significantly overexpressed in both hormone-naïve prostate cancer (HN-PC) and CRPC patients . CIP2A is also overexpressed, by 3- and 30-fold, in HN-PC and CRPC SCs respectively. In vivo binding of the AR to the intronic region of CIP2A and its functionality in the AR-moderate and AR-high expressing LNCaP cell-model systems is also demonstrated. Further, we show that AR positively regulates CIP2A expression, both at the mRNA and protein level. Finally, CIP2A depletion reduced cell viability and colony forming efficiency of AR-independent PPECs as well as AR-responsive LNCaP cells, in which anchorage-independent growth is also impaired.These findings identify CIP2A as a common denominator for AR-signaling and cancer SC functionality, highlighting its potential therapeutic significance in the most clinically challenging prostate pathology: castration-resistant prostate cancer. PMID:25965834

  15. Role of scaffolding protein CipC of Clostridium cellulolyticum in cellulose degradation.

    PubMed Central

    Pagès, S; Gal, L; Bélaïch, A; Gaudin, C; Tardif, C; Bélaïch, J P

    1997-01-01

    The role of a miniscaffolding protein, miniCipC1, forming part of Clostridium cellulolyticum scaffolding protein CipC in insoluble cellulose degradation was investigated. The parameters of the binding of miniCipC1, which contains a family III cellulose-binding domain (CBD), a hydrophilic domain, and a cohesin domain, to four insoluble celluloses were determined. At saturating concentrations, about 8.2 micromol of protein was bound per g of bacterial microcrystalline cellulose, while Avicel, colloidal Avicel, and phosphoric acid-swollen cellulose bound 0.28, 0.38, and 0.55 micromol of miniCipC1 per g, respectively. The dissociation constants measured varied between 1.3 x 10(-7) and 1.5 x 10(-8) M. These results are discussed with regard to the properties of the various substrates. The synergistic action of miniCipC1 and two forms of endoglucanase CelA (with and without the dockerin domain [CelA2 and CelA3, respectively]) in cellulose degradation was also studied. Although only CelA2 interacted with miniCipC1 (K(d), 7 x 10(-9) M), nonhydrolytic miniCipC1 enhanced the activities of endoglucanases CelA2 and CelA3 with all of the insoluble substrates tested. This finding shows that miniCipC1 plays two roles: it increases the enzyme concentration on the cellulose surface and enhances the accessibility of the enzyme to the substrate by modifying the structure of the cellulose, leading to an increased available cellulose surface area. In addition, the data obtained with a hybrid protein, CelA3-CBD(CipC), which was more active towards all of the insoluble substrates tested confirm that the CBD of the scaffolding protein plays an essential role in cellulose degradation. PMID:9139893

  16. Fundus Autofluorescence Imaging in an Ocular Screening Program

    PubMed Central

    Kolomeyer, A. M.; Nayak, N. V.; Szirth, B. C.; Khouri, A. S.

    2012-01-01

    Purpose. To describe integration of fundus autofluorescence (FAF) imaging into an ocular screening program. Methods. Fifty consecutive screening participants were included in this prospective pilot imaging study. Color and FAF (530/640 nm exciter/barrier filters) images were obtained with a 15.1MP Canon nonmydriatic hybrid camera. A clinician evaluated the images on site to determine need for referral. Visual acuity (VA), intraocular pressure (IOP), and ocular pathology detected by color fundus and FAF imaging modalities were recorded. Results. Mean ± SD age was 47.4 ± 17.3 years. Fifty-two percent were female and 58% African American. Twenty-seven percent had a comprehensive ocular examination within the past year. Mean VA was 20/39 in the right eye and 20/40 in the left eye. Mean IOP was 15 mmHg bilaterally. Positive color and/or FAF findings were identified in nine (18%) individuals with diabetic retinopathy or macular edema (n = 4), focal RPE defects (n = 2), age-related macular degeneration (n = 1), central serous retinopathy (n = 1), and ocular trauma (n = 1). Conclusions. FAF was successfully integrated in our ocular screening program and aided in the identification of ocular pathology. Larger studies examining the utility of this technology in screening programs may be warranted. PMID:23316224

  17. Free viewpoint image generation using multi-pass dynamic programming

    NASA Astrophysics Data System (ADS)

    Fukushima, Norishige; Yendo, Tomohiro; Fujii, Toshiaki; Tanimoto, Masayuki

    2007-02-01

    Ray-Space is categorized by Image-Based Rendering (IBR), thus generated views have photo-realistic quality. While this method has the performance of high quality imaging, this needs a lot of images or cameras. The reason why that is Ray-Space requires various direction's and position's views instead of 3D depth information. In this paper, we reduce that flood of information using view-centered ray interpolation. View-centered interpolation means estimating view dependent depth value (or disparity map) at generating view-point and interpolating that of pixel values using multi-view images and depth information. The combination of depth estimation and interpolation realizes the rendering photo-realistic images effectively. Unfortunately, however, if depth estimation is week or mistake, a lot of artifacts appear in creating images. Thus powerful depth estimation method is required. When we render the free viewpoint images video, we perform the depth estimation at every frame. Thus we want to keep a lid on computing cost. Our depth estimation method is based on dynamic programming (DP). This method optimizes and solves depth images at the weak matching area with high-speed performance. But scan-line noises become appeared because of the limit of DP. So, we perform the DP multi-direction pass and sum-up the result of multi-passed DPs. Our method fulfills the low computation cost and high depth estimation performance.

  18. Role of "oncogenic nexus" of CIP2A in breast oncogenesis: how does it work?

    PubMed

    De, Pradip; Carlson, Jennifer H; Leyland-Jones, Brian; Dey, Nandini

    2015-01-01

    The CIP2A gene is an oncogene associated with solid and hematologic malignancies [1]. CIP2A protein is an oncoprotein and a potential cancer therapy target [2]. Literature shows that CIP2A inhibits the tumor suppressor protein PP2A [3] which downregulates phophorylation of AKT, a hallmark of cancers [4] and stabilizes the proto-oncogene, c-MYC in tumor cells [5], the comprehensive action of CIP2A and its functional interaction(s) with other oncoproteins and tumor suppressors is not clearly established. Recently we tried to put forward a contextual mode-of-action of CIP2A protein in a review which proposed that CIP2A influences oncogenesis via an "oncogenic nexus" [1]. In this review we critically evaluated the potential relevance of the mode-of-action of the "oncogenic nexus" of CIP2A in breast carcinogenesis and appraised the role of this nexus in different PAM50 luminal A, PAM50 luminal B, PAM50 HER2-enriched and PAM50 basal BC. This review has a novel approach. Here we have not only compiled and discussed the latest developments in this field but also presented data obtained from c-BioPortal and STRING10 in order to substantiate our view regarding the mode-of-action of the "oncogenic nexus" of CIP2A. We functionally correlated alterations of genes pertaining to the "oncogenic nexus" of CIP2A with protein-protein interactions between the different components of the nexus including (1) subunits of PP2A, (2) multiple transcription factors including MYC oncogene and (3) components of the PI3K-mTOR and the MAPK-ERK oncogenic pathways. Using these proteins as "input" to STRING10 we studied the association, Action view, at the highest Confidence level. OncoPrints (c-BioPortal) showed alterations (%) of regulatory subunits genes of PP2A (PPP2R1A and PPP2R1B) along with alterations of CIP2A in breast invasive carcinoma (TCGA, Nature 2012 & TCGA, Provisional). Similar genetic alterations of PP2A were also observed in samples of breast tumors at our Avera Research

  19. Wideband electromagnetic scattering program. Fourier-based radar imaging techniques

    NASA Astrophysics Data System (ADS)

    Chan, B. L.; Young, J. D.; Rudduck, R. C.

    1993-09-01

    This report describes the implementation of Fourier based radar imaging algorithms in a computer program. In particular, the algorithms are derived for wide bandwidth and for specific geometries. These geometries are often measured by radar cross section measurement systems such as compact ranges and near field linear synthetic aperture radar systems. The limitations of different implementations of the algorithms are presented. Imaging results from radar measurements are also presented for an F-4 fighter aircraft, an M35 truck (1/16 scale model), and a forest.

  20. Cleaning validation 2: development and validation of an ion chromatographic method for the detection of traces of CIP-100 detergent.

    PubMed

    Resto, Wilfredo; Hernández, Darimar; Rey, Rosamil; Colón, Héctor; Zayas, José

    2007-05-01

    A cleaning validation method, ion chromatographic method with conductivity detection was developed and validated for the determination of traces of a clean-in-place (CIP) detergent. It was shown to be linear with a squared correlation coefficient (r(2)) of 0.9999 and average recoveries of 71.4% (area response factor) from stainless steel surfaces and 101% from cotton. The repeatability was found to be 2.17% and an intermediate precision of 1.88% across the range. The method was also shown to be sensitive with a detection limit (DL) of 0.13 ppm and a quantitation limit (QL) of 0.39 ppm for EDTA, which translates to less than 1 microL of CIP diluted in 100mL of diluent in both cases. The EDTA signal was well resolved from typical ions encountered in water samples or any other interference presented from swabs and surfaces. The method could be applied to cleaning validation samples. The validated method could be included as a suitable one for rapid and reliable cleaning validation program. PMID:17344013

  1. IMFIT: A Fast, Flexible New Program for Astronomical Image Fitting

    NASA Astrophysics Data System (ADS)

    Erwin, Peter

    2015-02-01

    I describe a new, open-source astronomical image-fitting program called IMFIT, specialized for galaxies but potentially useful for other sources, which is fast, flexible, and highly extensible. A key characteristic of the program is an object-oriented design that allows new types of image components (two-dimensional surface-brightness functions) to be easily written and added to the program. Image functions provided with IMFIT include the usual suspects for galaxy decompositions (Sérsic, exponential, Gaussian), along with Core-Sérsic and broken-exponential profiles, elliptical rings, and three components that perform line-of-sight integration through three-dimensional luminosity-density models of disks and rings seen at arbitrary inclinations. Available minimization algorithms include Levenberg-Marquardt, Nelder-Mead simplex, and Differential Evolution, allowing trade-offs between speed and decreased sensitivity to local minima in the fit landscape. Minimization can be done using the standard χ2 statistic (using either data or model values to estimate per-pixel Gaussian errors, or else user-supplied error images) or Poisson-based maximum-likelihood statistics; the latter approach is particularly appropriate for cases of Poisson data in the low-count regime. I show that fitting low-signal-to-noise ratio galaxy images using χ2 minimization and individual-pixel Gaussian uncertainties can lead to significant biases in fitted parameter values, which are avoided if a Poisson-based statistic is used; this is true even when Gaussian read noise is present.

  2. IMFIT: A FAST, FLEXIBLE NEW PROGRAM FOR ASTRONOMICAL IMAGE FITTING

    SciTech Connect

    Erwin, Peter

    2015-02-01

    I describe a new, open-source astronomical image-fitting program called IMFIT, specialized for galaxies but potentially useful for other sources, which is fast, flexible, and highly extensible. A key characteristic of the program is an object-oriented design that allows new types of image components (two-dimensional surface-brightness functions) to be easily written and added to the program. Image functions provided with IMFIT include the usual suspects for galaxy decompositions (Sérsic, exponential, Gaussian), along with Core-Sérsic and broken-exponential profiles, elliptical rings, and three components that perform line-of-sight integration through three-dimensional luminosity-density models of disks and rings seen at arbitrary inclinations. Available minimization algorithms include Levenberg-Marquardt, Nelder-Mead simplex, and Differential Evolution, allowing trade-offs between speed and decreased sensitivity to local minima in the fit landscape. Minimization can be done using the standard χ{sup 2} statistic (using either data or model values to estimate per-pixel Gaussian errors, or else user-supplied error images) or Poisson-based maximum-likelihood statistics; the latter approach is particularly appropriate for cases of Poisson data in the low-count regime. I show that fitting low-signal-to-noise ratio galaxy images using χ{sup 2} minimization and individual-pixel Gaussian uncertainties can lead to significant biases in fitted parameter values, which are avoided if a Poisson-based statistic is used; this is true even when Gaussian read noise is present.

  3. STEM image simulation with hybrid CPU/GPU programming.

    PubMed

    Yao, Y; Ge, B H; Shen, X; Wang, Y G; Yu, R C

    2016-07-01

    STEM image simulation is achieved via hybrid CPU/GPU programming under parallel algorithm architecture to speed up calculation on a personal computer (PC). To utilize the calculation power of a PC fully, the simulation is performed using the GPU core and multi-CPU cores at the same time to significantly improve efficiency. GaSb and an artificial GaSb/InAs interface with atom diffusion have been used to verify the computation. PMID:27093687

  4. Imfit: A Fast, Flexible Program for Astronomical Image Fitting

    NASA Astrophysics Data System (ADS)

    Erwin, Peter

    2014-08-01

    Imift is an open-source astronomical image-fitting program specialized for galaxies but potentially useful for other sources, which is fast, flexible, and highly extensible. Its object-oriented design allows new types of image components (2D surface-brightness functions) to be easily written and added to the program. Image functions provided with Imfit include Sersic, exponential, and Gaussian galaxy decompositions along with Core-Sersic and broken-exponential profiles, elliptical rings, and three components that perform line-of-sight integration through 3D luminosity-density models of disks and rings seen at arbitrary inclinations. Available minimization algorithms include Levenberg-Marquardt, Nelder-Mead simplex, and Differential Evolution, allowing trade-offs between speed and decreased sensitivity to local minima in the fit landscape. Minimization can be done using the standard chi^2 statistic (using either data or model values to estimate per-pixel Gaussian errors, or else user-supplied error images) or the Cash statistic; the latter is particularly appropriate for cases of Poisson data in the low-count regime. The C++ source code for Imfit is available under the GNU Public License.

  5. An interactive lake survey program. [airborne multispectral sensor image processing

    NASA Technical Reports Server (NTRS)

    Smith, A. Y.

    1977-01-01

    Consideration is given to the development and operation of the interactive lake survey program developed by the Jet Propulsion Laboratory and the Environmental Protection Agency. The program makes it possible to locate, isolate, and store any number of water bodies on the basis of a given digital image. The stored information may be used to generate statistical analyses of each body of water including the lake surface area and the shoreline perimeter. The hardware includes a 360/65 host computer, a Ramtek G100B display controller, and a trackball cursor. The system is illustrated by the LAKELOC operation as it would be applied to a Landsat scene, noting the FARINA and STATUS programs. The water detection algorithm, which increases the accuracy with which water and land data may be separated, is discussed.

  6. PC-based digital imaging for storing microscopy images for surveillance programs

    SciTech Connect

    Schleitweiler, P.M.; Ransick, M.H.

    1990-01-01

    Advances in video technology and digital imaging have resulted in the ability to produce an image in high quality digital format. Images that are created and viewed in a digital format can be quantitatively measured and then stored to disk for later examination. Write-once read many (WORM) optical disks are excellent media for permanently archiving digital images. One of the best applications for this image storage technology is storage of microscopy images for surveillance programs. Currently, the most common type of recording medium used in service laboratories is Polaroid instant developing film. The short developing time and ease in processing make it swell suited for most high volume laboratories. But the convenience of this film has a substantial price tag. Depending on the type of film, each picture can cost about $1.50. The two metallurgical support laboratories at Mound are frequently required to make multiple copies for design agencies or program managers. Permanent archiving of photos is required for both WR and surveillance samples. This has made Polaroid film a significant part of the expense budget. In addition, the thousands of photos taken annually present a considerable storage problem. 2 figs.

  7. Embossed radiography utilizing an image-shifting subtraction program

    NASA Astrophysics Data System (ADS)

    Sato, Eiichi; Osawa, Akihiro; Matsukiyo, Hiroshi; Enomoto, Toshiyuki; Watanabe, Manabu; Takahashi, Kiyomi; Sato, Shigehiro; Ogawa, Akira; Onagawa, Jun

    2010-07-01

    We developed an image-shifting subtraction program and carried out embossed radiography (ER) utilizing single- and dual-energy subtractions. In particular, dual-energy subtraction was carried out to decrease the absorption contrast of unnecessary regions. The contrast resolution of a target region was increased using the subtraction program and a linear-contrast system in a flat panel detector (FPD). The X-ray generator had a 100 μm-focus tube, and the subtractions were performed at tube voltages of 40 and 70 kV, a tube current of 0.50 mA, and an X-ray exposure time of 5.0 s. ER was achieved with cohesion imaging using the FPD with pixel sizes of 48×48 μm 2, and the shifting dimension of an object in the horizontal and vertical directions ranged from 48 to 96 μm. At a shifting distance of 96 μm, the spatial resolutions in the horizontal and vertical directions measured with a lead test chart were both 83 μm. In ER of animal phantoms, we obtained high-contrast embossed images of fine bones, gadolinium oxide particles in blood vessels, iodine-based microspheres in coronary arteries approximately 100 μm in diameter.

  8. Loss of the F-BAR protein CIP4 reduces platelet production by impairing membrane-cytoskeleton remodeling

    PubMed Central

    Chen, Yolande; Aardema, Jorie; Kale, Sayali; Whichard, Zakary L.; Awomolo, Arinola; Blanchard, Elisabeth; Chang, Brian; Myers, David R.; Ju, Lining; Tran, Reginald; Reece, David; Christensen, Hilary; Boukour, Siham; Debili, Najet; Strom, Ted S.; Rawlings, David; Vázquez, Francisco X.; Voth, Gregory A.; Zhu, Cheng; Kahr, Walter H. A.; Lam, Wilbur A.

    2013-01-01

    Megakaryocytes generate platelets through extensive reorganization of the cytoskeleton and plasma membrane. Cdc42 interacting protein 4 (CIP4) is an F-BAR protein that localizes to membrane phospholipids through its BAR domain and interacts with Wiskott-Aldrich Syndrome Protein (WASP) via its SRC homology 3 domain. F-BAR proteins promote actin polymerization and membrane tubulation. To study its function, we generated CIP4-null mice that displayed thrombocytopenia similar to that of WAS− mice. The number of megakaryocytes and their progenitors was not affected. However, the number of proplatelet protrusions was reduced in CIP4-null, but not WAS−, megakaryocytes. Electron micrographs of CIP4-null megakaryocytes showed an altered demarcation membrane system. Silencing of CIP4, not WASP, expression resulted in fewer proplatelet-like extensions. Fluorescence anisotropy studies showed that loss of CIP4 resulted in a more rigid membrane. Micropipette aspiration demonstrated decreased cortical actin tension in megakaryocytic cells with reduced CIP4 or WASP protein. These studies support a new biophysical mechanism for platelet biogenesis whereby CIP4 enhances the complex, dynamic reorganization of the plasma membrane (WASP independent) and actin cortex network (as known for WASP and cortical actin) to reduce the work required for generating proplatelets. CIP4 is a new component in the highly coordinated system of megakaryocytic membrane and cytoskeletal remodeling affecting platelet production. PMID:23881916

  9. Magnification Embossed Radiography Utilizing Image-Shifting Subtraction Program

    NASA Astrophysics Data System (ADS)

    Akihiro Osawa,; Manabu Watanabe,; Eiichi Sato,; Hiroshi Matsukiyo,; Toshiyuki Enomoto,; Jiro Nagao,; Purkhet Abderyim,; Katsuo Aizawa,; Etsuro Tanaka,; Hidezo Mori,; Toshiaki Kawai,; Akira Ogawa,; Kiyomi Takahashi,; Shigehiro Sato,; Jun Onagawa,

    2010-03-01

    We developed an image-shifting subtraction program and carried out magnification embossed radiography (MER) utilizing single- and dual-energy subtractions. In particular, dual-energy subtraction was carried out to decrease the absorption contrast of unnecessary regions. The contrast resolution of the target region was increased by the use of subtraction software and a linear-contrast system in a flat-panel detector (FPD). The X-ray generator had a 100-μm-focus tube, and the subtractions were performed at tube voltages of 40 and 70 kV, a tube current of 0.50 mA, and an X-ray exposure time of 5.0 s. MER images with threefold magnification were obtained using the FPD with a pixel size of 48× 48 μm2, and the shifting dimensions of the imaged object in the horizontal and vertical directions ranged from 48 to 192 μm. At a shifting distance ranging from 48 to 144 μm, the spatial resolutions in the horizontal and vertical directions measured with a lead test chart were both 50 μm. In the MER of nonliving animals, we obtained high-contrast embossed images of fine bones, gadolinium oxide particles in blood vessels, and iodine-based microspheres in coronary arteries of approximately 100 μm diameter.

  10. Magnification Embossed Radiography Utilizing Image-Shifting Subtraction Program

    NASA Astrophysics Data System (ADS)

    Osawa, Akihiro; Watanabe, Manabu; Sato, Eiichi; Matsukiyo, Hiroshi; Enomoto, Toshiyuki; Nagao, Jiro; Abderyim, Purkhet; Aizawa, Katsuo; Tanaka, Etsuro; Mori, Hidezo; Kawai, Toshiaki; Ogawa, Akira; Takahashi, Kiyomi; Sato, Shigehiro; Onagawa, Jun

    2010-03-01

    We developed an image-shifting subtraction program and carried out magnification embossed radiography (MER) utilizing single- and dual-energy subtractions. In particular, dual-energy subtraction was carried out to decrease the absorption contrast of unnecessary regions. The contrast resolution of the target region was increased by the use of subtraction software and a linear-contrast system in a flat-panel detector (FPD). The X-ray generator had a 100-µm-focus tube, and the subtractions were performed at tube voltages of 40 and 70 kV, a tube current of 0.50 mA, and an X-ray exposure time of 5.0 s. MER images with threefold magnification were obtained using the FPD with a pixel size of 48×48 µm2, and the shifting dimensions of the imaged object in the horizontal and vertical directions ranged from 48 to 192 µm. At a shifting distance ranging from 48 to 144 µm, the spatial resolutions in the horizontal and vertical directions measured with a lead test chart were both 50 µm. In the MER of nonliving animals, we obtained high-contrast embossed images of fine bones, gadolinium oxide particles in blood vessels, and iodine-based microspheres in coronary arteries of approximately 100 µm diameter.

  11. PUPA: a pulse programming assistant for NMR imaging

    SciTech Connect

    Foxvog, D.; Li, X.; Vargas, J.E.; Bourne, J.R.; Sztipanovits, J.; Mushlin, R.; Harrison, C.G.

    1987-12-01

    The design of pulse programs for magnetic resonance imaging (MRI) experiments is tedious and complex, requiring a deep understanding of the interactions that exist between magnetic fields generated during an MRI experiment. This paper describes an intelligent system that understands how to construct the multichannel temporal sequences of pulses needed to control an MRI experiment. PUPA, the PUlse Programmers Assistant, provides assistance to a relatively naive user of MRI systems. Knowledge is coded in the form of rules and semantic networks. A natural language facility and menu system are provided for communication with the user.

  12. Aurora B prevents delayed DNA replication and premature mitotic exit by repressing p21Cip1

    PubMed Central

    Trakala, Marianna; Fernández-Miranda, Gonzalo; Pérez de Castro, Ignacio; Heeschen, Christopher; Malumbres, Marcos

    2013-01-01

    Aurora kinase B is a critical component of the chromosomal passenger complex, which is involved in the regulation of microtubule-kinetochore attachments and cytokinesis. By using conditional knockout cells and chemical inhibition, we show here that inactivation of Aurora B results in delayed G1/S transition and premature mitotic exit. Aurora B deficiency results in delayed DNA replication in cultured fibroblasts as well as liver cells after hepatectomy. This is accompanied by increased transcription of the cell cycle inhibitor p21Cip1. Lack of Aurora B does not prevent mitotic entry but results in a premature exit from prometaphase in the presence of increased p21Cip1-Cdk1 inactive complexes. Aurora B-null cells display reduced degradation of cyclin B1, suggesting the presence of phenomenon known as adaptation to the mitotic checkpoint, previously described in yeast. Elimination of p21Cip1 rescues Cdk1 activity and prevents premature mitotic exit in Aurora B-deficient cells. These results suggest that Aurora B represses p21Cip1, preventing delayed DNA replication, Cdk inhibition and premature mitotic exit. The upregulation of p21Cip1 observed after inhibition of Aurora B may have important implications in cell cycle progression, tetraploidy, senescence or cancer therapy. PMID:23428904

  13. CIP-MOC Modeling of Seismic Wave Propagation in Elastic Media

    NASA Astrophysics Data System (ADS)

    Yoshimi, M.

    2004-12-01

    In many fields such as hydrodynamics and MHD, the CIP method, an upwind difference hyperbolic equation solver, has widely been employed for advection calculation. The CIP scheme was constructed considering that an advected property and its spatial derivative follow same advection equation. This effects low numerical dispersion and relaxed CFL condition in the advection calculation. In the present work, we developed a CIP-MOC (CIP with method of characteristics) scheme for seismic wave propagation in 3D elastic heterogeneous media with flat free surface. 3D elastic wave equations in velocity-stress formulation and their spatial derivatives, as well, are converted into sets of 1D advection equations and non-advection equations for each direction (x,y,z in Cartesian coodinate system) with the method of characteristics. Since the Riemann invariant of each advection equation consists of stress and velocity, updatings of velocity and stress are simultaneous and a collocated grid system is employed. A free surface is modeled as a zero-stress surface. A reflection free boundary is installed by considering no incident wave comes from outside of the boundary. A double coupled seismic point source is introduced as external point stresses. Overall scheme is made up of multiphases employing time-splitting and directional-splitting techniques. Each time step is composed of three directional updating phases each for wave propagation in x, y and z direction. Each directional updating phase is made up of advection phase and non-advection phase. In the advection phase, advection equations are solved with the CIP method. In the non-advection phases, non-advetion equations and boundary conditions are evaluated with central finite differences. We conducted CIP-MOC seismic wave propagation simulations in a half-space, layered and fully heterogeneous media for embedded point source. By comparing our products with those produced with discrete wavenumber method and finite difference method

  14. A numerical procedure — CIP — to solve all phases of matter together

    NASA Astrophysics Data System (ADS)

    Yabe, Takashi; Zhang, Yan; Xiao, Feng

    We have succeeded for the first time to simulate dynamic phase transition from metal to vapor. This success is due to the CIP (Cubic-Interporated Pseudoparticle/Propagation) method that can treat solid, liquid and gas together and can trace a sharp interface with almost one grid. The strategy of the CIP method and its various modifications are reviewed including application to various aspects of multi-phase flow such as laser-induced evaporation, slamming of ship and formation of milk crown in three dimensions.

  15. xdamp An IDL-based data and image manipulation program

    Energy Science and Technology Software Center (ESTSC)

    2002-06-26

    xdamp is a graphical user interface (GUI) designed to allow the user to manipulate two-dimensional waveforms (data vs. time) and images (usually digitized radiographic film or digital camera outputs)that are typical of electrical engineering applications. A typical single data set from these applications will generate ~ 100 time-dependent waveforms and possibly a few images. xdamp can manipulate waveforms both in time and in amplitude. Typical operations are: time shifting, truncating before or after a specificmore » time, adding, multiplying, integrating, and averaging. When manipulating images, the spatial dimensions are maintained as important data. Standard electrical engineering quantities (maximum, minimum, fully-width-at-half-maximum, rise-time, mean, standard deviation) are calculated for each waveform and automatically displayed. Annotation can be added to each waveform and/or image and the overall file so that the data contains full documentation. PostScript printing is supported. xdamp supports full audit trail information on each waveform. Data are saved using the Hierarchical Data Format (HDF) from the National Center for Supercomputing Applications. xdamp uses the Interactive Data Language (IDL) from Research Systems, Inc., a Xerox company, as the processing engine. The entire program is written inthe IDL macro language to enhance portability. IDL is currently supported on the macintosh, alpha computers, Windows-based computers, and on virtually all UNIX platforms. Portability to all of these platforms has been verified. xdamp has a full internal language for creating macros useful for repetitive data reduction and analysis. xdamp can manipulate waveforms both in time and in amplitude. Some advanced features included are: the ability to compare waveforms in time and amplitude, the ability to generate high-frequence cable compensators, both integration and differentiation of waveforms, Fourier transforms of waveforms, and automatic execution of

  16. xdamp An IDL-based data and image manipulation program

    SciTech Connect

    Ballard, William P.

    2002-06-26

    xdamp is a graphical user interface (GUI) designed to allow the user to manipulate two-dimensional waveforms (data vs. time) and images (usually digitized radiographic film or digital camera outputs)that are typical of electrical engineering applications. A typical single data set from these applications will generate ~ 100 time-dependent waveforms and possibly a few images. xdamp can manipulate waveforms both in time and in amplitude. Typical operations are: time shifting, truncating before or after a specific time, adding, multiplying, integrating, and averaging. When manipulating images, the spatial dimensions are maintained as important data. Standard electrical engineering quantities (maximum, minimum, fully-width-at-half-maximum, rise-time, mean, standard deviation) are calculated for each waveform and automatically displayed. Annotation can be added to each waveform and/or image and the overall file so that the data contains full documentation. PostScript printing is supported. xdamp supports full audit trail information on each waveform. Data are saved using the Hierarchical Data Format (HDF) from the National Center for Supercomputing Applications. xdamp uses the Interactive Data Language (IDL) from Research Systems, Inc., a Xerox company, as the processing engine. The entire program is written inthe IDL macro language to enhance portability. IDL is currently supported on the macintosh, alpha computers, Windows-based computers, and on virtually all UNIX platforms. Portability to all of these platforms has been verified. xdamp has a full internal language for creating macros useful for repetitive data reduction and analysis. xdamp can manipulate waveforms both in time and in amplitude. Some advanced features included are: the ability to compare waveforms in time and amplitude, the ability to generate high-frequence cable compensators, both integration and differentiation of waveforms, Fourier transforms of waveforms, and automatic execution of macros

  17. P21Cip1 is a critical mediator of the cytotoxic action of thymidylate synthase inhibitors in colorectal carcinoma cells.

    PubMed

    Geller, James I; Szekely-Szucs, Kinga; Petak, Istvan; Doyle, Belinda; Houghton, Janet A

    2004-09-01

    We have demonstrated previously that interferon (IFN)-gamma sensitizes human colon carcinoma cell lines to the cytotoxic effects of 5-fluorouracil combined with leucovorin and to the thymidylate synthase inhibitor, ZD9331, dependent on thymineless stress-induced DNA damage, independent of p53. Here we demonstrate that the cyclin-dependent kinase (CDK) inhibitor p21(Cip1) regulates thymineless stress-induced cytotoxicity in these cells. HCT116 wild-type (wt) and p53-/- cells underwent apoptosis and loss in clonogenic survival when exposed to ZD9331, whereas p21Cip1-/- cells were resistant. In contrast, IFN-gamma induced marked cytotoxicity in p21Cip1-/- cells only. ZD9331 induced p21Cip1 up-regulation in all of the cell lines examined, as did thymidine deprivation in thymidylate synthase-deficient (thymidylate synthase-) cells. Furthermore, selective induction of p21Cip1 in RKO was sufficient to induce apoptosis. P21Cip1, cdk1, cdk2, and cyclin E mRNA expression increased coincident with S-phase accumulation in HT29 cells treated with ZD9331 or 5fluorouracil/leucovorin, as demonstrated by cDNA microarray analyses. Cell cycle analyses revealed that HCT116 wt and p21Cip1 -/- cells accumulated in S phase within 24 h of ZD9331 exposure; however, wt cells exited S-phase more rapidly, where apoptosis occurred before mitosis, either in late S or G2. Finally, the CDK inhibitor roscovitine potentiated the cytotoxic activity of ZD9331 in both wt and p21Cip1-/- cells, strongly suggesting a role for p21Cip1-dependent CDK inhibition in cytotoxicity induced by thymidylate synthase inhibition. In summary, p21Cip1 positively regulates the cytotoxic action of thymidylate synthase inhibitors, negatively regulates the cytotoxic action of IFN-gamma, and enhances S-phase exit after thymineless stress, possibly via interaction with CDK-cyclin complexes. PMID:15342418

  18. The automatic surveillance, tracking, and imaging program. User manual

    NASA Astrophysics Data System (ADS)

    Szozda, Joe A.

    1989-03-01

    The automatic surveillance, tracking and imaging program (ASTIP) is a phased-array radar system which carries out surveillance of a designated field of view, and acquires and tracks multiple targets in its surveillance space. The user manual provides a detailed description of the device and file preparations that must be completed before ASTIP is run. The device and file preparations are summarized as an acquisition of resources list for quick reference by operators familiar with ASTIP. A detailed explanation of the preparations are presented in subsequent sections of the user manual. Each option in the menu-driven operator interface is discussed in detail. Diagrams of the ASTIP menus are located in the appendices. The error condition section explains the errors that are typically encountered and provides possible solutions to each of the problems. Finally, the procedures required to maintain the ASTIP software are explained in the building ASTIP section of the user manual.

  19. The teaching of computer programming and digital image processing in radiography.

    PubMed

    Allan, G L; Zylinski, J

    1998-06-01

    The increased use of digital processing techniques in Medical Radiations imaging modalities, along with the rapid advance in information technology has resulted in a significant change in the delivery of radiographic teaching programs. This paper details a methodology used to concurrently educate radiographers in both computer programming and image processing. The students learn to program in visual basic applications (VBA), and the programming skills are contextualised by requiring the students to write a digital subtraction angiography (DSA) package. Program code generation and image presentation interface is undertaken by the spreadsheet Microsoft Excel. The user-friendly nature of this common interface enables all students to readily begin program creation. The teaching of programming and image processing skills by this method may be readily generalised to other vocational fields where digital image manipulation is a professional requirement. PMID:9726504

  20. What Can the Community Involvement Program Tell Us about Alumni Giving at the University of the Pacific

    ERIC Educational Resources Information Center

    Ruiz-Huston, Ines Marta

    2010-01-01

    The purpose of this research is to analyze characteristics and motivators among alumni of the Community Involvement Program (CIP) for donating to the University of the Pacific. The research questions were: (1) what are the characteristics and motivators of CIP alumni for donating to the University of the Pacific? (2) do the characteristics and…

  1. Mesopause Horizontal wind estimates based on AIM CIPS polar mesospheric cloud pattern matching

    NASA Astrophysics Data System (ADS)

    Rong, P.; Yue, J.; Russell, J. M.; Gong, J.; Wu, D. L.; Randall, C. E.

    2013-12-01

    A cloud pattern matching approach is used to estimate horizontal winds in the mesopause region using Polar Mesospheric Cloud (PMC) albedo data measured by the Cloud Imaging and Particle Size instrument on the AIM satellite. Measurements for all 15 orbits per day throughout July 2007 are used to achieve statistical significance. For each orbit, eighteen out of the twenty-seven scenes are used for the pattern matching operation. Some scenes at the lower latitudes are not included because there is barely any cloud coverage for these scenes. The frame-size chosen is about 12 degrees in longitude and 3 degrees in latitude. There is no strict criterion in choosing the frame size since PMCs are widespread in the polar region and most local patterns do not have a clearly defined boundary. The frame moves at a step of 1/6th of the frame size in both the longitudinal and latitudinal directions to achieve as many 'snap-shots' as possible. A 70% correlation is used as a criterion to define an acceptable match between two patterns at two time frames; in this case the time difference is about 3.6 minutes that spans every 5 'bowtie' scenes. A 70% criterion appears weak if the chosen pattern is expected to act like a tracer. It is known that PMC brightness varies rapidly with a changing temperature and water vapor environment or changing nucleation conditions, especially on smaller spatial scales; therefore PMC patterns are not ideal tracers. Nevertheless, within a short time span such as 3.6 minutes a 70% correlation is sufficient to identify two cloud patterns that come from the same source region, although the two patterns may exhibit a significant difference in the actual brightness. Analysis of a large number of matched cloud patterns indicates that over the 3.6-minute time span about 70% of the patterns remain in the same locations. Given the 25-km2 horizontal resolution of CIPS data, this suggests that the overall magnitude of horizontal wind at PMC altitudes (~80-87 km) in

  2. Draft Genome Sequence of the Butyric Acid Producer Clostridium tyrobutyricum Strain CIP I-776 (IFP923)

    PubMed Central

    Clément, Benjamin; Lopes Ferreira, Nicolas

    2016-01-01

    Here, we report the draft genome sequence of Clostridium tyrobutyricum CIP I-776 (IFP923), an efficient producer of butyric acid. The genome consists of a single chromosome of 3.19 Mb and provides useful data concerning the metabolic capacities of the strain. PMID:26941139

  3. Structure of the catalytic domain of glucuronoyl esterase Cip2 from Hypocrea jecorina

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The structure of the catalytic domain of glucuronoyl esterase Cip2 from the fungus Hypocrea jecorina was determined at a resolution of 1.9 Angstroms. This is the first structure of the newly established carbohydrate esterase family 15. The structure has revealed the residues Ser278–His411–Glu301 pre...

  4. CIP4 promotes metastasis in triple-negative breast cancer and is associated with poor patient prognosis.

    PubMed

    Cerqueira, Otto L D; Truesdell, Peter; Baldassarre, Tomas; Vilella-Arias, Santiago A; Watt, Kathleen; Meens, Jalna; Chander, Harish; Osório, Cynthia A B; Soares, Fernando A; Reis, Eduardo M; Craig, Andrew W B

    2015-04-20

    Signaling via epidermal growth factor receptor (EGFR) and Src kinase pathways promote triple-negative breast cancer (TNBC) cell invasion and tumor metastasis. Here, we address the role of Cdc42-interacting protein-4 (CIP4) in TNBC metastasis in vivo, and profile CIP4 expression in human breast cancer patients. In human TNBC cells, CIP4 knock-down (KD) led to less sustained activation of Erk kinase and impaired cell motility compared to control cells. This correlated with significant defects in 3D invasion of surrounding extracellular matrix by CIP4 KD TNBC cells when grown as spheroid colonies. In mammary orthotopic xenograft assays using both human TNBC cells (MDA-MB-231, HCC 1806) and rat MTLn3 cells, CIP4 silencing had no overt effect on tumor growth, but significantly reduced the incidence of lung metastases in each tumor model. In human invasive breast cancers, high CIP4 levels was significantly associated with high tumor stage, TNBC and HER2 subtypes, and risk of progression to metastatic disease. Together, these results implicate CIP4 in promoting metastasis in TNBCs. PMID:25823823

  5. Interaction between the endoglucanase CelA and the scaffolding protein CipC of the Clostridium cellulolyticum cellulosome.

    PubMed Central

    Pagès, S; Belaich, A; Tardif, C; Reverbel-Leroy, C; Gaudin, C; Belaich, J P

    1996-01-01

    The 5' end of the cipC gene, coding for the N-terminal part of CipC, the scaffolding protein of Clostridium cellulolyticum ATCC 35319, was cloned and sequenced. It encodes a 586-amino-acid peptide, including several domains: a cellulose-binding domain, a hydrophilic domain, and two hydrophobic domains (cohesin domains). Sequence alignments showed that the N terminus of CipC and CbpA of C. cellulovorans ATCC 35296 have the same organization. The mini-CipC polypeptide, containing a cellulose-binding domain, hydrophilic domain 1, and cohesin domain 1, was overexpressed in Escherichia coli and purified. The interaction between endoglucanase CelA, with (CelA2) and without (CelA3) the characteristic clostridial C-terminal domain called the duplicated-segment or dockerin domain, and the mini-CipC polypeptide was monitored by two different methods: the interaction Western blotting (immunoblotting) method and binding assays with biotin-labeled protein. Among the various forms of CelA (CelA2, CelA3, and an intermediary form containing only part of the duplicated segment), only CelA2 was found to interact with cohesin domain 1 of CipC. The apparent equilibrium dissociation constant of the CelA2-mini-CipC complex was 7 x 10(-9)M, which indicates that there exists a high affinity between these two proteins. PMID:8636029

  6. Applications review for a Space Program Imaging Radar (SPIR)

    NASA Technical Reports Server (NTRS)

    Simonett, D. S.

    1976-01-01

    The needs, applications, user support, research, and theoretical studies of imaging radar are reviewed. The applications of radar in water resources, minerals and petroleum exploration, vegetation resources, ocean radar imaging, and cartography are discussed. The advantages of space imaging radar are presented, and it is recommended that imaging radar be placed on the space shuttle.

  7. A Program To Promote Positive Body Image: A 1-Year Follow-Up Evaluation.

    ERIC Educational Resources Information Center

    McVey, Gail L.; Davis, Ron

    2002-01-01

    Evaluated the effectiveness of a program designed to promote body image satisfaction and prevent eating problems in young adolescent girls over a 1-year period. Found no program effect. Found instead, significant increases in body image satisfaction and decreases in eating problem scores over time for participants in both the prevention and…

  8. LIVA : a data reduction program for line-imaging ORVIS measurements.

    SciTech Connect

    Ao, Tommy

    2009-06-01

    LIVA (Line-Imaging Velocimetry Analysis) is a program for reducing data of a line-imaging optically recording velocity interferometer system (ORVIS) diagnostic. LIVA uses the Fourier transform method to extract phase information from recorded streak camera images. The extracted phase shift is used to infer target velocity as a function of space and time. The program can be run in any current version of MATLAB (2008a or later) or as a Windows XP executable.

  9. Role of “oncogenic nexus” of CIP2A in breast oncogenesis: how does it work?

    PubMed Central

    De, Pradip; Carlson, Jennifer H; Leyland-Jones, Brian; Dey, Nandini

    2015-01-01

    The CIP2A gene is an oncogene associated with solid and hematologic malignancies [1]. CIP2A protein is an oncoprotein and a potential cancer therapy target [2]. Literature shows that CIP2A inhibits the tumor suppressor protein PP2A [3] which downregulates phophorylation of AKT, a hallmark of cancers [4] and stabilizes the proto-oncogene, c-MYC in tumor cells [5], the comprehensive action of CIP2A and its functional interaction(s) with other oncoproteins and tumor suppressors is not clearly established. Recently we tried to put forward a contextual mode-of-action of CIP2A protein in a review which proposed that CIP2A influences oncogenesis via an “oncogenic nexus” [1]. In this review we critically evaluated the potential relevance of the mode-of-action of the “oncogenic nexus” of CIP2A in breast carcinogenesis and appraised the role of this nexus in different PAM50 luminal A, PAM50 luminal B, PAM50 HER2-enriched and PAM50 basal BC. This review has a novel approach. Here we have not only compiled and discussed the latest developments in this field but also presented data obtained from c-BioPortal and STRING10 in order to substantiate our view regarding the mode-of-action of the “oncogenic nexus” of CIP2A. We functionally correlated alterations of genes pertaining to the “oncogenic nexus” of CIP2A with protein-protein interactions between the different components of the nexus including (1) subunits of PP2A, (2) multiple transcription factors including MYC oncogene and (3) components of the PI3K-mTOR and the MAPK-ERK oncogenic pathways. Using these proteins as “input” to STRING10 we studied the association, Action view, at the highest Confidence level. OncoPrints (c-BioPortal) showed alterations (%) of regulatory subunits genes of PP2A (PPP2R1A and PPP2R1B) along with alterations of CIP2A in breast invasive carcinoma (TCGA, Nature 2012 & TCGA, Provisional). Similar genetic alterations of PP2A were also observed in samples of breast tumors at our

  10. Study of the Career Intern Program. Task D Final Report, Volume I. A Comparison of Implementation Issues in YEDPA Programs.

    ERIC Educational Resources Information Center

    Stromquist, Nelly P.

    Five Youth Employment and Demonstration Program Act (YEDPA) programs are compared with the Career Intern Program (CIP) (an alternative high school program aiming to prepare dropouts, and those likely to abandon school, for employment) in terms of emphasizing issues related to program implementation. The YEDPA programs selected for comparison upon…

  11. The sonographic digital portfolio: a longitudinal ultrasound image tracking program

    PubMed Central

    2012-01-01

    Background Ultrasonography (US) at the medical student level is developing. As clinical skills and simulation centers expand, US equipment miniaturizes, and more students are exposed to ultrasound; a digital portfolio comprised of US images and videos may be useful in demonstrating experience and possibly competency. Methods Medical students participated in US curricula consisting of didactics and hands-on training. From 1 July 2006 to 30 June 2008, student images and videos were saved. Total images and videos were evaluated and catalogued. Results A total of 10,074 images and 1,227 videos were saved during the 2-year period. For the academic year 2006 to 2007, 159 medical students obtained 3,641 of the images (84.9%) and 270 of the videos (86.0%). First year students obtained 778 images and 20 videos; second year students, 1,174 images and 64 videos; third year students, 211 images and 20 videos; and fourth year students, 1,478 images and 166 videos. For the academic year 2007 to 2008, 222 medical students obtained 4,340 images (75%) and 619 videos (67.8%). First year students obtained 624 images and 109 videos; second year students, 555 images and 81 videos; third year students, 132 images and 14 videos; and fourth year students, 3,029 images and 415 videos. Conclusions The ultrasound digital portfolio allows medical students to collate and document their ultrasound experience. Currently, there is no requirement for ultrasound training, documentation of competency, or minimum numbers of US exams for medical education. The ultrasound digital portfolio may be a useful tool in documenting ultrasound proficiency. PMID:22871130

  12. NASA Sea Ice Validation Program for the Defense Meteorological Satellite Program Special Sensor Microwave Imager

    NASA Technical Reports Server (NTRS)

    Cavalieri, Donald J. (Editor); Crawford, John P.; Drinkwater, Mark R.; Emery, William J.; Eppler, Duane T.; Farmer, L. Dennis; Fowler, Charles W.; Goodberlet, Mark; Jentz, Robert R.; Milman, Andrew

    1992-01-01

    The history of the program is described along with the SSM/I sensor, including its calibration and geolocation correction procedures used by NASA, SSM/I data flow, and the NASA program to distribute polar gridded SSM/I radiances and sea ice concentrations (SIC) on CD-ROMs. Following a discussion of the NASA algorithm used to convert SSM/I radiances to SICs, results of 95 SSM/I-MSS Landsat IC comparisons for regions in both the Arctic and the Antarctic are presented. The Landsat comparisons show that the overall algorithm accuracy under winter conditions is 7 pct. on average with 4 pct. negative bias. Next, high resolution active and passive microwave image mosaics from coordinated NASA and Navy aircraft underflights over regions of the Beaufort and Chukchi seas in March 1988 were used to show that the algorithm multiyear IC accuracy is 11 pct. on average with a positive bias of 12 pct. Ice edge crossings of the Bering Sea by the NASA DC-8 aircraft were used to show that the SSM/I 15 pct. ice concentration contour corresponds best to the location of the initial bands at the ice edge. Finally, a summary of results and recommendations for improving the SIC retrievals from spaceborne radiometers are provided.

  13. Supporting image algebra in the Matlab programming language for compression research

    NASA Astrophysics Data System (ADS)

    Schmalz, Mark S.; Ritter, Gerhard X.; Wilson, Joseph N.; Hayden, Eric T.

    2009-08-01

    Image algebra is a rigorous, concise notation that unifies linear and nonlinear mathematics in the image domain. Image algebra was developed under DARPA and US Air Force sponsorship at University of Florida for over 15 years beginning in 1984. Image algebra has been implemented in a variety of programming languages designed specifically to support the development of image processing and computer vision programs. The University of Florida has been associated with implementations supporting the languages FORTRAN, Ada, Lisp, and C++. The latter implementation involved the implementation of a class library, iac++, that supported image algebra programming in C++. Since image processing and computer vision are generally performed with operands that are array-based, the MatlabTM programming language is ideal for implementing the common subset of image algebra. Objects include sets and set operations, images and operations on images, as well as templates and image-template convolution operations. This implementation has been found to be useful for research in data, image, and video compression, as described herein. Due to the widespread acceptance of the Matlab programming language in the computing community, this new implementation offers exciting possibilities for supporting a large group of users. The control over an object's computational resources provided to the algorithm designer by Matlab means that the image algebra Matlab (IAM) library can employ versatile representations for the operands and operations of the algebra. In this paper, we first outline the purpose and structure of image algebra, then present IAM notation in relationship to the preceding (IAC++) implementation. We then provide examples to show how IAM is more convenient and more readily supports efficient algorithm development. Additionally, we show how image algebra and IAM can be employed in compression algorithm development and analysis.

  14. Functional Heterologous Expression of an Engineered Full Length CipA from Clostridium thermocellum in Thermoanaerobacterium saccharolyticum

    SciTech Connect

    Currie, Devin; Herring, Christopher; Guss, Adam M; Olson, Daniel G.; Hogsett, David; Lynd, Lee R

    2013-01-01

    BACKGROUND: Cellulose is highly recalcitrant and thus requires a specialized suite of enzymes to solubilize it into fermentable sugars. In C. thermocellum, these extracellular enzymes are present as a highly active multi-component system known as the cellulosome. This study explores the expression of a critical C. thermocellum cellulosomal component in T. saccharolyticum as a step toward creating a thermophilic bacterium capable of consolidated bioprocessing by employing heterologously expressed cellulosomes. RESULTS: We developed an inducible promoter system based on the native T. saccharolyticum xynA promoter, which was shown to be induced by xylan and xylose. The promoter was used to express the cellulosomal component cipA*, an engineered form of the wild-type cipA from C. thermocellum. Expression and localization to the supernatant were both verified for CipA*. When a cipA mutant C. thermocellum strain was cultured with a CipA*-expressing T. saccharolyticum strain, hydrolysis and fermentation of 10 grams per liter SigmaCell 101, a highly crystalline cellulose, were observed. This trans-species complementation of a cipA deletion demonstrated the ability for CipA* to assemble a functional cellulosome. CONCLUSION: This study is the first example of an engineered thermophile heterologously expressing a structural component of a cellulosome. To achieve this goal we developed and tested an inducible promoter for controlled expression in T. saccharolyticum as well as a synthetic cipA. In addition, we demonstrate a high degree of hydrolysis (up to 93%) on microcrystalline cellulose.

  15. Overexpression of CIP2A is an independent prognostic indicator in nasopharyngeal carcinoma and its depletion suppresses cell proliferation and tumor growth

    PubMed Central

    2014-01-01

    Background Cancerous inhibitor of protein phosphatase 2A (CIP2A) is an oncoprotein that acts as a prognostic marker for several human malignancies. In this study, we investigated the clinical significance of CIP2A and its function in nasopharyngeal carcinoma (NPC). Methods Quantitative RT-PCR, western blot, and immunohistochemistry analyses were used to quantify CIP2A expression in NPC cell lines and clinical samples. Kaplan-Meier curves were used to estimate the association between CIP2A expression and patient survival. The functional role of CIP2A in NPC cell lines was evaluated by small interfering RNA-mediated depletion of the protein followed by analyses of cell proliferation and xenograft growth. Results CIP2A levels were upregulated in NPC cell lines and clinical samples at both the mRNA and protein levels (P < 0.01). Patients with high CIP2A expression had poorer overall survival (HR, 1.98; 95% CI, 1.16-3.34; P = 0.01) and poorer disease-free survival (HR, 1.68; 95% CI, 1.07-2.62; P = 0.02) rates than patients with low CIP2A expression. In addition, CIP2A expression status was an independent prognostic indicator for NPC patients. The depletion of CIP2A expression inhibited c-Myc protein expression in NPC cell lines, suppressed cell viability, colony formation, and anchorage-independent growth in vitro, and inhibited xenograft tumor growth in vivo. Conclusions Our data demonstrate that high CIP2A expression in patients was associated with poor survival in NPC, and depletion of CIP2A expression inhibited NPC cell proliferation and tumor growth. Thus, these results warrant further investigation of CIP2A as a novel therapeutic target for the treatment of NPC. PMID:24884612

  16. Assessment of diabetic teleretinal imaging program at the Portland Department of Veterans Affairs Medical Center.

    PubMed

    Tsan, Grace L; Hoban, Keely L; Jun, Weon; Riedel, Kevin J; Pedersen, Amy L; Hayes, John

    2015-01-01

    We conducted a retrospective chart review of 200 diabetic patients who had teleretinal imaging performed between January 1, 2010, and January 1, 2011, at Portland Department of Veterans Affairs (VA) Medical Center outpatient clinics to assess the effectiveness of the diabetic teleretinal imaging program. Twenty patients (10%) had diabetic retinopathy. Ninety percent of the available teleretinal imaging studies were of adequate quality for interpretation. In accordance with local VA policy at that time, all teleretinal imaging patients should have been referred for a dilated retinal examination the following year. Image readers referred 97.5% of the patients to eye clinics for subsequent eye examinations, but the imagers scheduled appointments for only 80% of these patients. The redundancy rate, i.e., patients who had an eye examination within the past 6 mo, was 11%; the duplicate recall rate, i.e., patients who had a second teleretinal imaging performed within 1 yr of the eye examination, was 37%. Rates of timely diabetic eye examinations at clinics with teleretinal imaging programs, particularly when teleretinal imaging and eye clinics were colocated at the same community-based outpatient clinic, were higher than those without a teleretinal imaging program. We concluded that the Portland VA Medical Center's teleretinal imaging program was successful in increasing the screening rate for diabetic retinopathy. PMID:26230276

  17. A COUPLED TH/NEUTRONICS/CRUD FRAMEWORK IN PREDICTION OF CIPS PHENOMENON

    SciTech Connect

    Ling Zou; Hongbin Zhang; Jess Gehin; Brendan Kochunas

    2012-04-01

    A coupled TH/Neutronics/CRUD framework, which is able to simulate the CRUD deposits impact on CIPS phenomenon, was described in this paper. This framework includes the coupling among three essential physics, thermal-hydraulics, CRUD and neutronics. The overall framework was implemented by using the CFD software STAR-CCM+, developing CRUD codes, and using the neutronics code DeCART. The coupling was implemented by exchanging data between softwares using intermediate exchange files. A typical 3 by 3 PWR fuel pin problem was solved under this framework. The problem was solved in a 12 months length period of time. Time-dependent solutions were provided, including CRUD deposits inventory and their distributions on fuels, boron hideout amount inside CRUD deposits, as well as power shape changing over time. The results clearly showed the power shape suppression in regions where CRUD deposits exist, which is a strong indication of CIPS phenomenon.

  18. FOR Allocation to Distribution Systems based on Credible Improvement Potential (CIP)

    NASA Astrophysics Data System (ADS)

    Tiwary, Aditya; Arya, L. D.; Arya, Rajesh; Choube, S. C.

    2016-06-01

    This paper describes an algorithm for forced outage rate (FOR) allocation to each section of an electrical distribution system subject to satisfaction of reliability constraints at each load point. These constraints include threshold values of basic reliability indices, for example, failure rate, interruption duration and interruption duration per year at load points. Component improvement potential measure has been used for FOR allocation. Component with greatest magnitude of credible improvement potential (CIP) measure is selected for improving reliability performance. The approach adopted is a monovariable method where one component is selected for FOR allocation and in the next iteration another component is selected for FOR allocation based on the magnitude of CIP. The developed algorithm is implemented on sample radial distribution system.

  19. Higher-order schemes with CIP method and adaptive Soroban grid towards mesh-free scheme

    NASA Astrophysics Data System (ADS)

    Yabe, Takashi; Mizoe, Hiroki; Takizawa, Kenji; Moriki, Hiroshi; Im, Hyo-Nam; Ogata, Youichi

    2004-02-01

    A new class of body-fitted grid system that can keep the third-order accuracy in time and space is proposed with the help of the CIP (constrained interpolation profile/cubic interpolated propagation) method. The grid system consists of the straight lines and grid points moving along these lines like abacus - Soroban in Japanese. The length of each line and the number of grid points in each line can be different. The CIP scheme is suitable to this mesh system and the calculation of large CFL (>10) at locally refined mesh is easily performed. Mesh generation and searching of upstream departure point are very simple and almost mesh-free treatment is possible. Adaptive grid movement and local mesh refinement are demonstrated.

  20. Oncogenic nexus of cancerous inhibitor of protein phosphatase 2A (CIP2A): An oncoprotein with many hands

    PubMed Central

    De, Pradip; Carlson, Jennifer; Leyland-Jones, Brian; Dey, Nandini

    2014-01-01

    Oncoprotein CIP2A a Cancerous Inhibitor of PP2A forms an “oncogenic nexus” by virtue of its control on PP2A and MYC stabilization in cancer cells. The expression and prognostic function of CIP2A in different solid tumors including colorectal carcinoma, head & neck cancers, gastric cancers, lung carcinoma, cholangiocarcinoma, esophageal cancers, pancreatic carcinoma, brain cancers, breast carcinoma, bladder cancers, ovarian carcinoma, renal cell carcinomas, tongue cancers, cervical carcinoma, prostate cancers, and oral carcinoma as well as a number of hematological malignancies are just beginning to emerge. Herein, we reviewed the recent progress in our understanding of (1) how an “oncogenic nexus” of CIP2A participates in the tumorigenic transformation of cells and (2) how we can prospect/view the clinical relevance of CIP2A in the context of cancer therapy. The review will try to understand the role of CIP2A (a) as a biomarker in cancers and evaluate the prognostic value of CIP2A in different cancers (b) as a therapeutic target in cancers and (c) in drug response and developing chemo-resistance in cancers. PMID:25015035

  1. Oncogenic nexus of cancerous inhibitor of protein phosphatase 2A (CIP2A): an oncoprotein with many hands.

    PubMed

    De, Pradip; Carlson, Jennifer; Leyland-Jones, Brian; Dey, Nandini

    2014-07-15

    Oncoprotein CIP2A a Cancerous Inhibitor of PP2A forms an "oncogenic nexus" by virtue of its control on PP2A and MYC stabilization in cancer cells. The expression and prognostic function of CIP2A in different solid tumors including colorectal carcinoma, head and neck cancers, gastric cancers, lung carcinoma, cholangiocarcinoma, esophageal cancers, pancreatic carcinoma, brain cancers, breast carcinoma, bladder cancers, ovarian carcinoma, renal cell carcinomas, tongue cancers, cervical carcinoma, prostate cancers, and oral carcinoma as well as a number of hematological malignancies are just beginning to emerge. Herein, we reviewed the recent progress in our understanding of (1) how an "oncogenic nexus" of CIP2A participates in the tumorigenic transformation of cells and (2) how we can prospect/view the clinical relevance of CIP2A in the context of cancer therapy. The review will try to understand the role of CIP2A (a) as a biomarker in cancers and evaluate the prognostic value of CIP2A in different cancers (b) as a therapeutic target in cancers and (c) in drug response and developing chemo-resistance in cancers. PMID:25015035

  2. The AFGL image reconstruction program 2 speckle interferometry

    NASA Astrophysics Data System (ADS)

    Worden, S. P.; Hege, E. K.; Hubbard, E. N.; Woolf, N. J.; Strittmatter, P. A.

    1981-12-01

    Recent work indicates that large-telescope, optics-limited images are recoverable for objects as faint as +15 stellar magnitudes using a technique called speckle interferometry. This report presents a review of speckle interferometry, including current status and results. Section 2 provides a background of the Fourier mathematics used in image processing and optical systems. Section 3 describes how the atmosphere degrades astronomical images and how speckle interferometry has been used to recover high-resolution detail. Section 4 describes new work to recover actual optics-limited images, and compares active optics systems with speckle interferometry.

  3. Electromagnetic and absorbing property of CIPs/resin composite using the 3D forming process

    NASA Astrophysics Data System (ADS)

    Xu, Yonggang; Liang, Zichang; Wang, Xiaobing; Yuan, Liming; Li, Xinghao

    2016-08-01

    The absorbing composite filled with the flaky carbonyl iron particles (CIPs) were prepared using a three-dimensional (3D) forming process, in which the forming powder was fabricated using a milling process. The surface morphology was characterized by the scanning electron microscopy, the static magnetic property was evaluated on a vibrating sample magnetometer, and X-ray diffraction (XRD) patterns were done to analyze the particle crystal grain structure. The complex permittivity and permeability were measured using a vector network analyzer in the frequency range of 4-18 GHz. With the variable thickness was set, the reflection loss (RL) was simulated to analyze the absorbing property of the composite. The results showed that the forming powder was uniformly dispersed in the absorber, and the saturation magnetization and the grain structure of the CIPs in the forming powder nearly did not change in the milling process. With the same volume content CIPs added, the average permittivity and the imaginary permeability of the samples added the powder was smaller than the directly mixing sample due to the aggregation effect. The RL results showed that the absorbing composites using the 3D forming process with thickness 6 or 8 mm had an better absorbing property (minimum RL -13.58 and -21.85 dB) in 4-18 GHz.

  4. Aurora B Overexpression Causes Aneuploidy and p21Cip1 Repression during Tumor Development

    PubMed Central

    González-Loyola, Alejandra; Fernández-Miranda, Gonzalo; Trakala, Marianna; Partida, David; Samejima, Kumiko; Ogawa, Hiromi; Cañamero, Marta; de Martino, Alba; Martínez-Ramírez, Ángel; de Cárcer, Guillermo; Pérez de Castro, Ignacio; Earnshaw, William C.

    2015-01-01

    Aurora kinase B, one of the three members of the mammalian Aurora kinase family, is the catalytic component of the chromosomal passenger complex, an essential regulator of chromosome segregation in mitosis. Aurora B is overexpressed in human tumors although whether this kinase may function as an oncogene in vivo is not established. Here, we report a new mouse model in which expression of the endogenous Aurkb locus can be induced in vitro and in vivo. Overexpression of Aurora B in cultured cells induces defective chromosome segregation and aneuploidy. Long-term overexpression of Aurora B in vivo results in aneuploidy and the development of multiple spontaneous tumors in adult mice, including a high incidence of lymphomas. Overexpression of Aurora B also results in a reduced DNA damage response and decreased levels of the p53 target p21Cip1 in vitro and in vivo, in line with an inverse correlation between Aurora B and p21Cip1 expression in human leukemias. Thus, overexpression of Aurora B may contribute to tumor formation not only by inducing chromosomal instability but also by suppressing the function of the cell cycle inhibitor p21Cip1. PMID:26240282

  5. Planetary instrument definition and development program: 'Miniature Monochromatic Imager'

    NASA Technical Reports Server (NTRS)

    Broadfoot, A. L.

    1991-01-01

    The miniature monochromatic imager (MMI) development work became the basis for the preparation of several instruments which were built and flown on the shuttle STS-39 as well as being used in ground based experiments. The following subject areas are covered: (1) applications of the ICCD to airglow and auroral measurements and (2) a panchromatic spectrograph with supporting monochromatic imagers.

  6. The image/poetry education and public outreach program

    NASA Astrophysics Data System (ADS)

    Odenwald, Sten F.; Taylor, William W. L.; Reiff, Patricia H.

    2000-01-01

    Education and public outreach are viewed by NASA as significant undertakings for all of its space missions. The IMAGE satellite is one of the first missions to explicitly include `E&PO' in its original proposal to NASA in 1996. We will discuss what IMAGE has accomplished in this area to date, and what new activities it will conduct following a successful launch.

  7. Mississippi Curriculum Framework for Business and Office and Related Technology Cluster. Office Systems Technology (CIP: 52.0401--Administrative Assistant/Secretarial). Accounting Technology (CIP: 52.0302). Medical Office Technology (CIP: 52.0404--Medical Admin. Asst./Secretarial). Microcomputer Technology (CIP: 52.0490). Court Reporting Technology (CIP: 52.0405). Paralegal Technology (CIP: Paralegal/Legal Assistant).

    ERIC Educational Resources Information Center

    Mississippi Research and Curriculum Unit for Vocational and Technical Education, State College.

    This document, which is intended for use by community and junior colleges throughout Mississippi, contains curriculum frameworks for four programs in the postsecondary-level business and office cluster (office systems, accounting, medical office, and microcomputer technologies) and two programs in the legal cluster (court reporting and paralegal…

  8. CIP's Eighth Annual Educational Software Contest: The Winners.

    ERIC Educational Resources Information Center

    Donnelly, Denis

    1997-01-01

    Announces the winners of an annual software contest for innovative software in physics education. Winning entries include an application to help students visualize the origin of energy bands in a solid, a package on the radioastronomy of pulsars, and a school-level science simulation program. Also includes student winners, honorable mentions,…

  9. Fast imaging applications in the Nuclear Test Program

    SciTech Connect

    Lear, R.

    1983-10-14

    Applications of fast imaging employ both streak cameras and fast framing techniques. Image intensifier tubes are gated to provide fast two-dimensional shutters of 2 to 3 ns duration with shatter ratios of greater than 10/sup 6/ and resolution greater than 10/sup 4/ pixels. Shutters of less than 1 ns have been achieved with experimental tubes. Characterization data demonstrate the importance of tube and pulser design. Streak cameras are used to simultaneously record temporal and intensity information from up to 200 spatial points. Streak cameras are combined with remote readout for downhole uses and are coupled to fiber optic cables for uphole uses. Optical wavelength multiplexing is being studied as a means of compressing additional image data onto optical fibers. Performance data demonstrate trade-offs between image resolution and system sensitivity.

  10. NIH Roadmap & the Common Fund - Cancer Imaging Program

    Cancer.gov

    The NIH Roadmap for Medical Research is a series of high impact, trans-NIH programs supported by the NIH Common Fund. These programs address challenges that are priorities for the NIH and medical research but are issues that require the cooperation of more than one NIH institute to address.

  11. Genome image programs: visualization and interpretation of Escherichia coli microarray experiments.

    PubMed Central

    Zimmer, Daniel P; Paliy, Oleg; Thomas, Brian; Gyaneshwar, Prasad; Kustu, Sydney

    2004-01-01

    We have developed programs to facilitate analysis of microarray data in Escherichia coli. They fall into two categories: manipulation of microarray images and identification of known biological relationships among lists of genes. A program in the first category arranges spots from glass-slide DNA microarrays according to their position in the E. coli genome and displays them compactly in genome order. The resulting genome image is presented in a web browser with an image map that allows the user to identify genes in the reordered image. Another program in the first category aligns genome images from two or more experiments. These images assist in visualizing regions of the genome with common transcriptional control. Such regions include multigene operons and clusters of operons, which are easily identified as strings of adjacent, similarly colored spots. The images are also useful for assessing the overall quality of experiments. The second category of programs includes a database and a number of tools for displaying biological information about many E. coli genes simultaneously rather than one gene at a time, which facilitates identifying relationships among them. These programs have accelerated and enhanced our interpretation of results from E. coli DNA microarray experiments. Examples are given. PMID:15342544

  12. Going the Distance: Taking a Diagnostic Imaging Program to Frontier and Rural Oregon

    ERIC Educational Resources Information Center

    Malosh, Ann; Mallory, Stacy; Olson, Marcene

    2009-01-01

    The Grow Your Own diagnostic imaging program is a public/private collaborative venture involving the efforts of an array of community colleges, employers, workforce, and educational partners throughout Oregon. This statewide Community College Partnership delivers diagnostic imaging education to Oregon's rural communities via distributed learning…

  13. Interactions of ciprofloxacin (CIP), titanium dioxide (TiO2) nanoparticles and natural organic matter (NOM) in aqueous suspensions.

    PubMed

    Fries, Elke; Crouzet, Catherine; Michel, Caroline; Togola, Anne

    2016-09-01

    The aim of the present study was to investigate interactions of the antibiotic ciprofloxacin (CIP), titanium dioxide nanoparticles (TiO2 NP) and natural organic matter (NOM) in aqueous suspensions. The mean hydrodynamic diameter of particles of TiO2 NP and NOM in the suspensions ranged from 113 to 255nm. During batch experiments the radioactivity resulting from (14)CIP was determined in the filtrate (filter pore size 100nm) by scintillation measurements. Up to 72h, no significant sorption of NOM to TiO2 NP was observed at a TiO2 NP concentration of 5mg/L. When the concentration of TiO2 NP was increased to 500mg/L, a small amount of NOM of 9.5%±0.6% was sorbed at 72h. The low sorption affinity of NOM on TiO2 NP surfaces could be explained by the negative charge of both components in alkaline media or by the low hydrophobicity of the NOM contents. At a TiO2 NP concentration of 5mgL(-1), the sorption of CIP on TiO2 NP was insignificant (TiO2 NP/CIP ratio: 10). When the TiO2 NP/CIP ratio was increased to 1000, a significant amount of 53.6%±7.2% of CIP was sorbed on TiO2 NP under equilibrium conditions at 64h. In alkaline media, CIP is present mainly as zwitterions which have an affinity to sorb on negatively charged TiO2 NP surfaces. The sorption of CIP on TiO2 NP in the range of TiO2 NP concentrations currently estimated for municipal wastewater treatment plants is estimated to be rather low. The Freundlich sorption coefficients (KF) in the presence of NOM of 2167L(n)mgmg(-n)kg(-1) was about 10 times lower than in the absence of NOM. This is an indication that the particle fraction of NOM<100nm could play a role as a carrier for ionic organic micro-pollutants as CIP. PMID:26765511

  14. Applications notice for participation in the LANDSAT-D image data quality analysis program

    NASA Technical Reports Server (NTRS)

    1981-01-01

    The applications notice for the LANDSAT 4 image data quality analysis program is presented. The objectives of the program are to qualify LANDSAT 4 sensor and systems performance from a user applications point of view, and to identify any malfunctions that may impact data applications. Guidelines for preparing proposals and background information are provided.

  15. PIXTOOLS - 13 PROGRAMS AND A LIBRARY THAT OPERATE ON BITMAP IMAGE FILES

    NASA Technical Reports Server (NTRS)

    Choi, D.

    1994-01-01

    PIXTOOLS is a package for the Silicon Graphics IRIS consisting of thirteen programs plus a library for operating on bitmap images. The image data structure is referred to as a PIXIMAGE. The programs allow the IRIS user to create and edit, perform screen saves, resize, and capture high-resolution images from the Sharp JX450 scanner. Images can be output to the QMS laser printer, the Tektronix 4693 color thermal printer, and the Matrix QCRZ film recorder. Additionally, PIX format images can be converted to SGI image format (and vice versa) or converted to PostScript format. PIX or SGI format images can be converted to ".ras" files which can be read by the "rasp" routine in the PLOT3D/AMES program (available from COSMIC), and ".ras" files can be converted to PIX files. Eleven of the programs print information and read and write files while two, PIXSCAN and PIXEDIT, offer graphical interfaces. PIXEDIT uses the full IRIS screen as a drawing area and pop-up menus are available. The menus allow manipulation of images and background color, and saving the screen to a file. PIXSCAN is the user interface to the Sharp JX450 scanner. This program allows the user to do a preliminary scan of an image at low resolution, and then select an area to rescan in higher resolution into a file. PIXSCAN requires the user to have the "gpib" (IEEE 488) board and "libgpib.a" library from Silicon Graphics, Inc. User instructions for all the programs are provided in the form of UNIX on-line manual pages. The PIXTOOLS programs are written in C-Language for execution on SGI IRIS 4D series workstations running IRIX 3.2 or later. PIXEDIT (the largest program) requires 840K of main memory. The programs with graphical interfaces require that the IRIS have at least 24 bit planes. The program package is available on a .25 inch streaming magnetic tape cartridge in UNIX tar format. A README file and UNIX man pages provide information regarding installation and use of the PIXTOOLS programs. A nine

  16. CIP4 coordinates with phospholipids and actin-associated proteins to localize to the protruding edge and produce actin ribs and veils.

    PubMed

    Saengsawang, Witchuda; Taylor, Kendra L; Lumbard, Derek C; Mitok, Kelly; Price, Amanda; Pietila, Lauren; Gomez, Timothy M; Dent, Erik W

    2013-06-01

    Cdc42-interacting protein 4 (CIP4), a member of the F-BAR family of proteins, plays important roles in a variety of cellular events by regulating both membrane and actin dynamics. In many cell types, CIP4 functions in vesicle formation, endocytosis and membrane tubulation. However, recent data indicate that CIP4 is also involved in protrusion in some cell types, including cancer cells (lamellipodia and invadopodia) and neurons (ribbed lamellipodia and veils). In neurons, CIP4 localizes specifically to extending protrusions and functions to limit neurite outgrowth early in development. The mechanism by which CIP4 localizes to the protruding edge membrane and induces lamellipodial/veil protrusion and actin rib formation is not known. Here, we show that CIP4 localization to the protruding edge of neurons is dependent on both the phospholipid content of the plasma membrane and the underlying organization of actin filaments. Inhibiting phosphatidylinositol (3,4,5)-trisphosphate (PIP3) production decreases CIP4 at the membrane. CIP4 localization to the protruding edge is also dependent on Rac1/WAVE1, rather than Cdc42/N-WASP. Capping actin filaments with low concentrations of cytochalasin D or by overexpressing capping protein dramatically decreases CIP4 at the protruding edge, whereas inactivating Arp2/3 drives CIP4 to the protruding edge. We also demonstrate that CIP4 dynamically colocalizes with Ena/VASP and DAAM1, two proteins known to induce unbranched actin filament arrays and play important roles in neuronal development. Together, this is the first study to show that the localization of an F-BAR protein depends on both actin filament architecture and phospholipids at the protruding edge of developing neurons. PMID:23572514

  17. The Crystal Structure of the Core Domain of a Cellulose Induced Protein (Cip1) from Hypocrea jecorina, at 1.5 Å Resolution

    PubMed Central

    Jacobson, Frida; Karkehabadi, Saeid; Hansson, Henrik; Goedegebuur, Frits; Wallace, Louise; Mitchinson, Colin; Piens, Kathleen; Stals, Ingeborg; Sandgren, Mats

    2013-01-01

    In an effort to characterise the whole transcriptome of the fungus Hypocrea jecorina, cDNA clones of this fungus were identified that encode for previously unknown proteins that are likely to function in biomass degradation. One of these newly identified proteins, found to be co-regulated with the major H. jecorina cellulases, is a protein that was denoted Cellulose induced protein 1 (Cip1). This protein consists of a glycoside hydrolase family 1 carbohydrate binding module connected via a linker region to a domain with yet unknown function. After cloning and expression of Cip1 in H. jecorina, the protein was purified and biochemically characterised with the aim of determining a potential enzymatic activity for the novel protein. No hydrolytic activity against any of the tested plant cell wall components was found. The proteolytic core domain of Cip1 was then crystallised, and the three-dimensional structure of this was determined to 1.5 Å resolution utilising sulphur single-wavelength anomalous dispersion phasing (sulphor-SAD). A calcium ion binding site was identified in a sequence conserved region of Cip1 and is also seen in other proteins with the same general fold as Cip1, such as many carbohydrate binding modules. The presence of this ion was found to have a structural role. The Cip1 structure was analysed and a structural homology search was performed to identify structurally related proteins. The two published structures with highest overall structural similarity to Cip1 found were two poly-lyases: CsGL, a glucuronan lyase from H. jecorina and vAL-1, an alginate lyase from the Chlorella virus. This indicates that Cip1 may be a lyase. However, initial trials did not detect significant lyase activity for Cip1. Cip1 is the first structure to be solved of the 23 currently known Cip1 sequential homologs (with a sequence identity cut-off of 25%), including both bacterial and fungal members. PMID:24039705

  18. Biomedical image analysis using Markov random fields & efficient linear programing.

    PubMed

    Komodakis, Nikos; Besbes, Ahmed; Glocker, Ben; Paragios, Nikos

    2009-01-01

    Computer-aided diagnosis through biomedical image analysis is increasingly considered in health sciences. This is due to the progress made on the acquisition side, as well as on the processing one. In vivo visualization of human tissues where one can determine both anatomical and functional information is now possible. The use of these images with efficient intelligent mathematical and processing tools allows the interpretation of the tissues state and facilitates the task of the physicians. Segmentation and registration are the two most fundamental tools in bioimaging. The first aims to provide automatic tools for organ delineation from images, while the second focuses on establishing correspondences between observations inter and intra subject and modalities. In this paper, we present some recent results towards a common formulation addressing these problems, called the Markov Random Fields. Such an approach is modular with respect to the application context, can be easily extended to deal with various modalities, provides guarantees on the optimality properties of the obtained solution and is computationally efficient. PMID:19963682

  19. Mindseye: a visual programming and modeling environment for imaging science

    NASA Astrophysics Data System (ADS)

    Carney, Thom

    1998-07-01

    Basic vision science research has reached the point that many investigators are now designing quantitative models of human visual function in areas such as, pattern discrimination, motion detection, optical flow, color discrimination, adaptation and stereopsis. These models have practical significance in their application to image compression technologies and as tools for evaluating image quality. We have been working on a vision modeling environment, called Mindseye, that is designed to simplify the implementation and testing of general purpose spatio- temporal models of human vision. Mindseye is an evolving general-purpose vision-modeling environment that embodies the general structures of the visual system and provides a set of modular tools within a flexible platform tailored to the needs of researchers. The environment employs a user- friendly graphics interface with on-line documentation that describes the functionality of the individual modules. Mindseye, while functional, is still research in progress. We are seeking input from the image compression and evaluation community as well as from the vision science community as to the potential utility of Mindseye, and how it might be enhanced to meet future needs.

  20. A new algorithm to reduce noise in microscopy images implemented with a simple program in python.

    PubMed

    Papini, Alessio

    2012-03-01

    All microscopical images contain noise, increasing when (e.g., transmission electron microscope or light microscope) approaching the resolution limit. Many methods are available to reduce noise. One of the most commonly used is image averaging. We propose here to use the mode of pixel values. Simple Python programs process a given number of images, recorded consecutively from the same subject. The programs calculate the mode of the pixel values in a given position (a, b). The result is a new image containing in (a, b) the mode of the values. Therefore, the final pixel value corresponds to that read in at least two of the pixels in position (a, b). The application of the program on a set of images obtained by applying salt and pepper noise and GIMP hurl noise with 10-90% standard deviation showed that the mode performs better than averaging with three-eight images. The data suggest that the mode would be more efficient (in the sense of a lower number of recorded images to process to reduce noise below a given limit) for lower number of total noisy pixels and high standard deviation (as impulse noise and salt and pepper noise), while averaging would be more efficient when the number of varying pixels is high, and the standard deviation is low, as in many cases of Gaussian noise affected images. The two methods may be used serially. PMID:21898664

  1. Programs Offered and Programs Completed at North Dakota Institutions of Postsecondary Education, July 1, 2008-June 30, 2009

    ERIC Educational Resources Information Center

    Schepp, Julie; Domagala, Anna

    2009-01-01

    This report provides information on degree and certificate programs offered and student program completions for fiscal year 2008-09 in North Dakota's public and private postsecondary educational institutions. Institutional programs are coded in accordance with the Classification of Instructional Programs (CIP Code) system provided by the National…

  2. Program for PET image alignment: Effects on calculated differences in cerebral metabolic rates for glucose

    SciTech Connect

    Phillips, R.L.; London, E.D.; Links, J.M.; Cascella, N.G. )

    1990-12-01

    A program was developed to align positron emission tomography images from multiple studies on the same subject. The program allowed alignment of two images with a fineness of one-tenth the width of a pixel. The indications and effects of misalignment were assessed in eight subjects from a placebo-controlled double-blind crossover study on the effects of cocaine on regional cerebral metabolic rates for glucose. Visual examination of a difference image provided a sensitive and accurate tool for assessing image alignment. Image alignment within 2.8 mm was essential to reduce variability of measured cerebral metabolic rates for glucose. Misalignment by this amount introduced errors on the order of 20% in the computed metabolic rate for glucose. These errors propagate to the difference between metabolic rates for a subject measured in basal versus perturbed states.

  3. Lapatinib inhibits CIP2A/PP2A/p-Akt signaling and induces apoptosis in triple negative breast cancer cells

    PubMed Central

    Liu, Chun-Yu; Hu, Ming-Hung; Hsu, Chia-Jung; Huang, Chun-Teng; Wang, Duen-Shian; Tsai, Wen-Chun; Chen, Yi-Ting; Lee, Chia-Han; Chu, Pei-Yi; Hsu, Chia-Chi; Chen, Ming-Huang; Shiau, Chung-Wai; Tseng, Ling-Ming; Chen, Kuen-Feng

    2016-01-01

    We tested the efficacy of lapatinib, a dual tyrosine kinase inhibitor which interrupts the HER2 and epidermal growth factor receptor (EGFR) pathways, in a panel of triple-negative breast cancer (TNBC) cells, and examined the drug mechanism. Lapatinib showed an anti-proliferative effect in HCC 1937, MDA-MB-468, and MDA-MB-231 cell lines. Lapatinib induced significant apoptosis and inhibited CIP2A and p-Akt in a dose and time-dependent manner in the three TNBC cell lines. Overexpression of CIP2A reduced lapatinib-induced apoptosis in MDA-MB-468 cells. In addition, lapatinib increased PP2A activity (in relation to CIP2A inhibition). Moreover, lapatinib-induced apoptosis and p-Akt downregulation was attenuated by PP2A antagonist okadaic acid. Furthermore, lapatinib indirectly decreased CIP2A transcription by disturbing the binding of Elk1 to the CIP2A promoter. Importantly, lapatinib showed anti-tumor activity in mice bearing MDA-MB-468 xenograft tumors, and suppressed CIP2A as well as p-Akt in these xenografted tumors. In summary, inhibition of CIP2A determines the effects of lapatinib-induced apoptosis in TNBC cells. In addition to being a dual tyrosine kinase inhibitor of HER2 and EGFR, lapatinib also inhibits CIP2A/PP2A/p-Akt signaling in TNBC cells. PMID:26824320

  4. Loss of Notch1-dependent p21Waf1/Cip1 expression influences the Notch1 outcome in tumorigenesis

    PubMed Central

    Cialfi, Samantha; Palermo, Rocco; Manca, Sonia; De Blasio, Carlo; Vargas Romero, Paula; Checquolo, Saula; Bellavia, Diana; Uccelletti, Daniela; Saliola, Michele; D'Alessandro, Angelo; Zolla, Lello; Gulino, Alberto; Screpanti, Isabella; Talora, Claudio

    2014-01-01

    Notch signaling plays a complex role in carcinogenesis, and its signaling pathway has both tumor-suppressor and oncogenic components. In this study we investigated the effects of reactive oxygen species (ROS) on Notch1 signaling outcome in keratinocyte biology. We demonstrate that Notch1 function contributes to the arsenic-induced keratinocyte transformation. We found that acute exposure to arsenite increases oxidative stress and inhibits proliferation of keratinocyte cells by upregulation of p21waf1/Cip1. The necessity of p21waf1/Cip1 for arsenite-induced cell death was demonstrated by targeted downregulation of p21waf1/Cip1 by using RNA interference. We further demonstrated that on acute exposure to arsenite, p21waf1/Cip1 is upregulated and Notch1 downmodulated, whereas on chronic exposure to arsenite, malignant progression of arsenite-treated keratinocytes cells was accompanied by regained expression and activity of Notch1. Notch1 activity in arsenite-transformed keratinocytes inhibits arsenite-induced upregulation of p21waf1/Cip1 by sustaining c-myc expression. We further demonstrated that c-myc collaborates with Nrf2, a key regulator for the maintenance of redox homeostasis, to promote metabolic activities that support cell proliferation and cytoprotection. Therefore, Notch1-mediated repression of p21waf1/Cip1 expression results in the inhibition of cell death and keratinocytes transformation. Our results not only demonstrate that sustained Notch1 expression is at least one key event implicated in the arsenite human skin carcinogenic effect, but also may provide mechanistic insights into the molecular aspects that determine whether Notch signaling will be either oncogenic or tumor suppressive. PMID:24801890

  5. A new fast accurate nonlinear medical image registration program including surface preserving regularization.

    PubMed

    Gruslys, Audrunas; Acosta-Cabronero, Julio; Nestor, Peter J; Williams, Guy B; Ansorge, Richard E

    2014-11-01

    Recently inexpensive graphical processing units (GPUs) have become established as a viable alternative to traditional CPUs for many medical image processing applications. GPUs offer the potential of very significant improvements in performance at low cost and with low power consumption. One way in which GPU programs differ from traditional CPU programs is that increasingly elaborate calculations per voxel may not impact of the overall processing time because memory accesses can dominate execution time. This paper presents a new GPU based elastic image registration program named Ezys. The Ezys image registration algorithm belongs to the wide class of diffeomorphic demons but uses surface preserving image smoothing and regularization filters designed for a GPU that would be computationally expensive on a CPU. We describe the methods used in Ezys and present results from two important neuroscience applications. Firstly inter-subject registration for transfer of anatomical labels and secondly longitudinal intra-subject registration to quantify atrophy in individual subjects. Both experiments showed that Ezys registration compares favorably with other popular elastic image registration programs. We believe Ezys is a useful tool for neuroscience and other applications, and also demonstrates the value of developing of novel image processing filters specifically designed for GPUs. PMID:24968094

  6. Preparation of methoxyl poly(ethylene glycol) (MPEG)-coated carbonyl iron particles (CIPs) and their application in potassium dihydrogen phosphate (KDP) magnetorheological finishing (MRF)

    NASA Astrophysics Data System (ADS)

    Ji, Fang; Xu, Min; Wang, Baorui; Wang, Chao; Li, Xiaoyuan; Zhang, Yunfei; Zhou, Ming; Huang, Wen; Wei, Qilong; Tang, Guangping; He, Jianguo

    2015-10-01

    KDP is a common type of optics that is extremely difficult to polish by the conventional route. MRF is a local polishing technology based on material removal via shearing with minimal normal load and sub-surface damage. In contrast to traditional emendation on an abrasive, the MPEG soft coating is designed and prepared to modify the CIP surface to achieve a hardness matched with that of KDP because CIP inevitably takes part in the material removal during finishing. Morphology and infrared spectra are explored to prove the existence of homogeneous coating, and the improvement of MPEG for the polishing quality is validated by the analysis of roughness, turning grooves, and stress. The synthesized MPEG-coated CIP (MPEG-CIP) is chemically and physically compatible with KDP, which can be removed after cleaning. Our research exhibits the promising prospects of MPEG-CIP in KDP MRF.

  7. Bioaerosol sampling by a personal rotating cup sampler CIP 10-M.

    PubMed

    Görner, Peter; Fabriès, Jean-François; Duquenne, Philippe; Witschger, Olivier; Wrobel, Richard

    2006-01-01

    High concentrations of bioaerosols containing bacterial, fungal and biotoxinic matter are encountered in many workplaces, e.g. solid waste treatment plants, waste water treatment plants and sewage networks. A personal bioaerosol sampler, the CIP 10-M (M-microbiologic), has been developed to measure worker exposure to airborne biological agents. This sampler is battery operated; it is light and easy to wear and offers full work shift autonomy. It can sample much higher concentrations than biological impactors and limits the mechanical stress on the microorganisms. Biological particles are collected in 2 ml of liquid medium inside a rotating cup fitted with radial vanes to maintain an air flow rate of 10 l min(-1) at a rotational speed of approximately 7,000 rpm. The rotating cup is made of sterilisable material. The sampled particles follow a helicoidal trajectory as they are pushed to the surface of the liquid by centrifugal force, which creates a thin vertical liquid layer. Sterile water or another collecting liquid can be used. Three particle size selectors allow health-related aerosol fractions to be sampled according to international conventions. The sampled microbiological particles can be easily recovered for counting, incubation or further biochemical analysis, e.g., for airborne endotoxins. Its physical sampling efficiency was laboratory tested and field trials were carried out in industrial waste management conditions. The results indicate satisfactory collection efficiency, whilst experimental application has demonstrated the usefulness of the CIP 10-M personal sampler for individual bioaerosol exposure monitoring. PMID:16395458

  8. Scientific Programs and Funding Opportunities at the National Institute of Biomedical Imaging and Bioengineering

    NASA Astrophysics Data System (ADS)

    Baird, Richard

    2006-03-01

    The mission of the National Institute of Biomedical Imaging and Bioengineering (NIBIB) is to improve human health by promoting the development and translation of emerging technologies in biomedical imaging and bioengineering. To this end, NIBIB supports a coordinated agenda of research programs in advanced imaging technologies and engineering methods that enable fundamental biomedical discoveries across a broad spectrum of biological processes, disorders, and diseases and have significant potential for direct medical application. These research programs dramatically advance the Nation's healthcare by improving the detection, management and, ultimately, the prevention of disease. The research promoted and supported by NIBIB also is strongly synergistic with other NIH Institutes and Centers as well as across government agencies. This presentation will provide an overview of the scientific programs and funding opportunities supported by NIBIB, highlighting those that are of particular important to the field of medical physics.

  9. The National Cancer Informatics Program (NCIP) Annotation and Image Markup (AIM) Foundation model.

    PubMed

    Mongkolwat, Pattanasak; Kleper, Vladimir; Talbot, Skip; Rubin, Daniel

    2014-12-01

    Knowledge contained within in vivo imaging annotated by human experts or computer programs is typically stored as unstructured text and separated from other associated information. The National Cancer Informatics Program (NCIP) Annotation and Image Markup (AIM) Foundation information model is an evolution of the National Institute of Health's (NIH) National Cancer Institute's (NCI) Cancer Bioinformatics Grid (caBIG®) AIM model. The model applies to various image types created by various techniques and disciplines. It has evolved in response to the feedback and changing demands from the imaging community at NCI. The foundation model serves as a base for other imaging disciplines that want to extend the type of information the model collects. The model captures physical entities and their characteristics, imaging observation entities and their characteristics, markups (two- and three-dimensional), AIM statements, calculations, image source, inferences, annotation role, task context or workflow, audit trail, AIM creator details, equipment used to create AIM instances, subject demographics, and adjudication observations. An AIM instance can be stored as a Digital Imaging and Communications in Medicine (DICOM) structured reporting (SR) object or Extensible Markup Language (XML) document for further processing and analysis. An AIM instance consists of one or more annotations and associated markups of a single finding along with other ancillary information in the AIM model. An annotation describes information about the meaning of pixel data in an image. A markup is a graphical drawing placed on the image that depicts a region of interest. This paper describes fundamental AIM concepts and how to use and extend AIM for various imaging disciplines. PMID:24934452

  10. 12 CFR 1292.5 - Community Investment Cash Advance Programs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 10 2014-01-01 2014-01-01 false Community Investment Cash Advance Programs... COMMUNITY INVESTMENT CASH ADVANCE PROGRAMS § 1292.5 Community Investment Cash Advance Programs. (a) In... shall offer a CIP to provide financing for housing projects and for eligible targeted community...

  11. Applied high-speed imaging for the icing research program at NASA Lewis Research Center

    NASA Technical Reports Server (NTRS)

    Slater, Howard; Owens, Jay; Shin, Jaiwon

    1991-01-01

    The Icing Research Tunnel at NASA Lewis Research Center provides scientists a scaled, controlled environment to simulate natural icing events. The closed-loop, low speed, refrigerated wind tunnel offers the experimental capability to test for icing certification requirements, analytical model validation and calibration techniques, cloud physics instrumentation refinement, advanced ice protection systems, and rotorcraft icing methodology development. The test procedures for these objectives all require a high degree of visual documentation, both in real-time data acquisition and post-test image processing. Information is provided to scientific, technical, and industrial imaging specialists as well as to research personnel about the high-speed and conventional imaging systems will be on the recent ice protection technology program. Various imaging examples for some of the tests are presented. Additional imaging examples are available from the NASA Lewis Research Center's Photographic and Printing Branch.

  12. Applied high-speed imaging for the icing research program at NASA Lewis Research Center

    NASA Technical Reports Server (NTRS)

    Slater, Howard; Owens, Jay; Shin, Jaiwon

    1992-01-01

    The Icing Research Tunnel at NASA Lewis Research Center provides scientists a scaled, controlled environment to simulate natural icing events. The closed-loop, low speed, refrigerated wind tunnel offers the experimental capability to test for icing certification requirements, analytical model validation and calibration techniques, cloud physics instrumentation refinement, advanced ice protection systems, and rotorcraft icing methodology development. The test procedures for these objectives all require a high degree of visual documentation, both in real-time data acquisition and post-test image processing. Information is provided to scientific, technical, and industrial imaging specialists as well as to research personnel about the high-speed and conventional imaging systems will be on the recent ice protection technology program. Various imaging examples for some of the tests are presented. Additional imaging examples are available from the NASA Lewis Research Center's Photographic and Printing Branch.

  13. Dynamical programming based turbulence velocimetry for fast visible imaging of tokamak plasma.

    PubMed

    Banerjee, Santanu; Zushi, H; Nishino, N; Mishra, K; Onchi, T; Kuzmin, A; Nagashima, Y; Hanada, K; Nakamura, K; Idei, H; Hasegawa, M; Fujisawa, A

    2015-03-01

    An orthogonal dynamic programming (ODP) based particle image velocimetry (PIV) technique is developed to measure the time resolved flow field of the fluctuating structures at the plasma edge and scrape off layer (SOL) of tokamaks. This non-intrusive technique can provide two dimensional velocity fields at high spatial and temporal resolution from a fast framing image sequence and hence can provide better insights into plasma flow as compared to conventional probe measurements. Applicability of the technique is tested with simulated image pairs. Finally, it is applied to tangential fast visible images of QUEST plasma to estimate the SOL flow in inboard poloidal null-natural divertor configuration. This technique is also applied to investigate the intricate features of the core of the run-away dominated phase following the injection of a large amount of neutrals in the target Ohmic plasma. Development of the ODP-PIV code and its applicability on actual plasma images is reported. PMID:25832227

  14. Dynamical programming based turbulence velocimetry for fast visible imaging of tokamak plasma

    NASA Astrophysics Data System (ADS)

    Banerjee, Santanu; Zushi, H.; Nishino, N.; Mishra, K.; Onchi, T.; Kuzmin, A.; Nagashima, Y.; Hanada, K.; Nakamura, K.; Idei, H.; Hasegawa, M.; Fujisawa, A.

    2015-03-01

    An orthogonal dynamic programming (ODP) based particle image velocimetry (PIV) technique is developed to measure the time resolved flow field of the fluctuating structures at the plasma edge and scrape off layer (SOL) of tokamaks. This non-intrusive technique can provide two dimensional velocity fields at high spatial and temporal resolution from a fast framing image sequence and hence can provide better insights into plasma flow as compared to conventional probe measurements. Applicability of the technique is tested with simulated image pairs. Finally, it is applied to tangential fast visible images of QUEST plasma to estimate the SOL flow in inboard poloidal null-natural divertor configuration. This technique is also applied to investigate the intricate features of the core of the run-away dominated phase following the injection of a large amount of neutrals in the target Ohmic plasma. Development of the ODP-PIV code and its applicability on actual plasma images is reported.

  15. Managing complex processing of medical image sequences by program supervision techniques

    NASA Astrophysics Data System (ADS)

    Crubezy, Monica; Aubry, Florent; Moisan, Sabine; Chameroy, Virginie; Thonnat, Monique; Di Paola, Robert

    1997-05-01

    Our objective is to offer clinicians wider access to evolving medical image processing (MIP) techniques, crucial to improve assessment and quantification of physiological processes, but difficult to handle for non-specialists in MIP. Based on artificial intelligence techniques, our approach consists in the development of a knowledge-based program supervision system, automating the management of MIP libraries. It comprises a library of programs, a knowledge base capturing the expertise about programs and data and a supervision engine. It selects, organizes and executes the appropriate MIP programs given a goal to achieve and a data set, with dynamic feedback based on the results obtained. It also advises users in the development of new procedures chaining MIP programs.. We have experimented the approach for an application of factor analysis of medical image sequences as a means of predicting the response of osteosarcoma to chemotherapy, with both MRI and NM dynamic image sequences. As a result our program supervision system frees clinical end-users from performing tasks outside their competence, permitting them to concentrate on clinical issues. Therefore our approach enables a better exploitation of possibilities offered by MIP and higher quality results, both in terms of robustness and reliability.

  16. 2005 Mississippi Curriculum Framework: Postsecondary Gerontology Technology. (Program CIP: 19.0702 - Gerontology)

    ERIC Educational Resources Information Center

    Boulton, Mary

    2005-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  17. 2011 Mississippi Curriculum Framework: Postsecondary Forestry Technology. (Program CIP: 03.0511 - Forest Technology/Technician)

    ERIC Educational Resources Information Center

    Jones, Luke; Keeton, Jeff

    2011-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  18. 2007 Mississippi Curriculum Framework: Postsecondary Forestry Technology. (Program CIP: 03.0511 - Forest Technology/Technician)

    ERIC Educational Resources Information Center

    Johnson, Carl; Loden, Emily; Keeton, Jeff; Thompson, Don; Mitchell, Brian; Couch, Andrew; Walker, Bob; Grado, Steve

    2007-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  19. 2010 Mississippi Curriculum Framework: Postsecondary Physical Therapist Assistant. (Program CIP: 51.0806 - Physical Therapist Assistant)

    ERIC Educational Resources Information Center

    Hester, Tom; Chapman, Pamela; Crowson, Patti; Ennis, Kimberly

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  20. 2009 Mississippi Curriculum Framework: Postsecondary Occupational Safety and Health Technology. Program CIP: 15.0701

    ERIC Educational Resources Information Center

    Flowers, Walter M.

    2009-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  1. 2009 Mississippi Curriculum Framework: Postsecondary Respiratory Care Technology. (Program CIP: 51.0908 - Respiratory Care Practitioner)

    ERIC Educational Resources Information Center

    Anderson, Lori; Brooks, Anthony; Miller, Shirley; Newell, Jim; Prince, Beverly; Smith, Jacqueline; Smith, Lynn; Ware, Chris

    2009-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  2. 2009 Mississippi Curriculum Framework: Postsecondary Ophthalmic Technology. (Program CIP-51.1801 - Opticianry/Dispensing Optician)

    ERIC Educational Resources Information Center

    Sciple, Eddie

    2009-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  3. 2006 Mississippi Curriculum Framework: Postsecondary Physical Therapist Assistant. (Program CIP: 51.0806 - Physical Therapist Assistant)

    ERIC Educational Resources Information Center

    Hester, Tom; Latham, Lisa; Crowson, Patti; Ennis, Kimberly

    2006-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  4. 2011 Mississippi Curriculum Framework: Postsecondary Dental Hygiene Technology. (Program CIP: 51.0602 - Dental Hygienist)

    ERIC Educational Resources Information Center

    Alexander, Nick; Dunn, Catherine; Hill, Stanley; Lindsay, William

    2011-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  5. 2008 Mississippi Curriculum Framework: Postsecondary Pharmacy Technology. (Program CIP: 51.0805 - Pharmacy Technician/Assistant)

    ERIC Educational Resources Information Center

    Juergens, John; Sanders, Marsha

    2008-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  6. 2009 Mississippi Curriculum Framework: Postsecondary Medical Assisting Technology. (Program CIP-51.0801 - Medical /Clinical Assisting)

    ERIC Educational Resources Information Center

    Roberson, Kaye; King, Christine

    2009-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  7. 2010 Mississippi Curriculum Framework: Postsecondary Paralegal Technology. (Program CIP: 22.0302 - Legal Assistant/Paralegal)

    ERIC Educational Resources Information Center

    Cox, Janet; Dinkins, Shivochie L.; Dozier, Darleen; Ford, Kathryn; Hinton, Amy; McDavid, Stephan; Phifer-Starks, Kim D.; Winston, Felicia

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  8. 2006 Mississippi Curriculum Framework: Postsecondary Paralegal Technology. (Program CIP: 22.0302 - Legal Assistant/Paralegal)

    ERIC Educational Resources Information Center

    Hinton, Amy; Phifer-Starks, Kim D.; Nelson, LaTricia

    2006-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  9. 2010 Mississippi Curriculum Framework: Postsecondary Surgical Technology. (Program CIP: 51.0909 - Surgical Technology/Technologist)

    ERIC Educational Resources Information Center

    Davis, Tonya; Elliott, Jessica; Gandy, Zielda; Binkley, Dottie; Wilburn, Cathy; Ladner, Melissa; Howell, Karen; Shirley, LeAnn; Hinton, Debbie; Allhoff, Tammy

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  10. 2006 Mississippi Curriculum Framework: Postsecondary Surgical Technology. (Program CIP: 51.0909 - Surgical Technology/Technologist)

    ERIC Educational Resources Information Center

    Tice, Tonya; Elliott, Jessica; Gandy, Zielda; Wilkerson, Tammy

    2006-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  11. 2007 Mississippi Curriculum Framework: Postsecondary Occupational Therapy Assistant. (Program CIP: 51.0803 - Occupational Therapist Assistant)

    ERIC Educational Resources Information Center

    Hager, Sherry; Pulver, Tim; Chittom, Suzanne

    2007-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  12. 2011 Mississippi Curriculum Framework: Postsecondary Occupational Therapy Assistant. (Program CIP: 51.0803 - Occupational Therapist Assistant)

    ERIC Educational Resources Information Center

    Hager, Sherry; Pulver, Tim; Chittom, Suzanne

    2011-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  13. 2009 Mississippi Curriculum Framework: Postsecondary Cardiovascular Technology. (Program CIP: 51.0901 - Cardiovascular Technology)

    ERIC Educational Resources Information Center

    Stanford-Means, Cynthia; Stevens, Richard

    2009-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  14. 2011 Mississippi Curriculum Framework: Postsecondary Paramedic. (Program CIP: 51.0904 - Emergency Medical Technology/Technician)

    ERIC Educational Resources Information Center

    Briscoe, Lisa; Bryant, Katrina; Deschamp, Clyde; Galtelli, Mark; Glasson, Kristi; Hall, David; Hood, Brenda; Mahaffey, Libby; McBryde, John; Read, John; Shirley, Gary

    2011-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  15. 2010 Mississippi Curriculum Framework: Postsecondary Clothing and Textile Services. (Program CIP-19.0905)

    ERIC Educational Resources Information Center

    Redfield, Verlene

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  16. Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase.

    PubMed

    Villa, Fabrizio; Simon, Aline C; Ortiz Bazan, Maria Angeles; Kilkenny, Mairi L; Wirthensohn, David; Wightman, Mel; Matak-Vinkovíc, Dijana; Pellegrini, Luca; Labib, Karim

    2016-08-01

    Replisome assembly at eukaryotic replication forks connects the DNA helicase to DNA polymerases and many other factors. The helicase binds the leading-strand polymerase directly, but is connected to the Pol α lagging-strand polymerase by the trimeric adaptor Ctf4. Here, we identify new Ctf4 partners in addition to Pol α and helicase, all of which contain a "Ctf4-interacting-peptide" or CIP-box. Crystallographic analysis classifies CIP-boxes into two related groups that target different sites on Ctf4. Mutations in the CIP-box motifs of the Dna2 nuclease or the rDNA-associated protein Tof2 do not perturb DNA synthesis genome-wide, but instead lead to a dramatic shortening of chromosome 12 that contains the large array of rDNA repeats. Our data reveal unexpected complexity of Ctf4 function, as a hub that connects multiple accessory factors to the replisome. Most strikingly, Ctf4-dependent recruitment of CIP-box proteins couples other processes to DNA synthesis, including rDNA copy-number regulation. PMID:27397685

  17. IL-6 modulates hepatocyte proliferation via induction of HGF/p21{sup cip1}: Regulation by SOCS3

    SciTech Connect

    Sun Rui; Jaruga, Barbara; Kulkarni, Shailin; Sun Haoyu; Gao Bin . E-mail: bgao@mail.nih.gov

    2005-12-30

    The precise role of IL-6 in liver regeneration and hepatocyte proliferation is controversial and the role of SOCS3 in liver regeneration remains unknown. Here we show that in vitro treatment with IL-6 inhibited primary mouse hepatocyte proliferation. IL-6 induced p21{sup cip1} protein expression in primary mouse hepatocytes. Disruption of the p21{sup cip1} gene abolished the inhibitory effect of IL-6 on cell proliferation. Co-culture with nonparenchymal liver cells diminished IL-6 inhibition of hepatocyte proliferation, which was likely due to IL-6 stimulation of nonparenchymal cells to produce HGF. Finally, IL-6 induced higher levels of p21{sup cip1} protein expression and a slightly stronger inhibition of cell proliferation in SOCS3{sup +/-} mouse hepatocytes compared to wild-type hepatocytes, while liver regeneration was enhanced and prolonged in SOCS3{sup +/-} mice. Our findings suggest that IL-6 directly inhibits hepatocyte proliferation via a p21{sup cip1}-dependent mechanism and indirectly enhances hepatocyte proliferation via stimulating nonparenchymal cells to produce HGF. SOCS3 negatively regulates liver regeneration.

  18. Label-free quantitative phosphoproteomics with novel pairwise abundance normalization reveals synergistic RAS and CIP2A signaling

    PubMed Central

    Kauko, Otto; Laajala, Teemu Daniel; Jumppanen, Mikael; Hintsanen, Petteri; Suni, Veronika; Haapaniemi, Pekka; Corthals, Garry; Aittokallio, Tero; Westermarck, Jukka; Imanishi, Susumu Y.

    2015-01-01

    Hyperactivated RAS drives progression of many human malignancies. However, oncogenic activity of RAS is dependent on simultaneous inactivation of protein phosphatase 2A (PP2A) activity. Although PP2A is known to regulate some of the RAS effector pathways, it has not been systematically assessed how these proteins functionally interact. Here we have analyzed phosphoproteomes regulated by either RAS or PP2A, by phosphopeptide enrichment followed by mass-spectrometry-based label-free quantification. To allow data normalization in situations where depletion of RAS or PP2A inhibitor CIP2A causes a large uni-directional change in the phosphopeptide abundance, we developed a novel normalization strategy, named pairwise normalization. This normalization is based on adjusting phosphopeptide abundances measured before and after the enrichment. The superior performance of the pairwise normalization was verified by various independent methods. Additionally, we demonstrate how the selected normalization method influences the downstream analyses and interpretation of pathway activities. Consequently, bioinformatics analysis of RAS and CIP2A regulated phosphoproteomes revealed a significant overlap in their functional pathways. This is most likely biologically meaningful as we observed a synergistic survival effect between CIP2A and RAS expression as well as KRAS activating mutations in TCGA pan-cancer data set, and synergistic relationship between CIP2A and KRAS depletion in colony growth assays. PMID:26278961

  19. Multi-Dimensional Assessment of Classroom Instructional Practice: A Validity Study of the Classroom Instructional Practice Scale (CIPS).

    ERIC Educational Resources Information Center

    Shim, Minsuk K.; Felner, Robert D.; Shim, Eunjae; Noonan, Nancy

    This study examined the reliability and validity of self-reported survey data on instructional practices. It was based on a nationwide survey of more than 25,000 teachers in more than 1,000 schools across 5 years. The survey instrument was the Classroom Instructional Practice Scale (CIPS), which was based on the Classroom Information Sheet…

  20. Student Evaluation of Teaching Effectiveness of a Nationwide Innovative Education Program on Image Display Technology

    ERIC Educational Resources Information Center

    Yueh, Hsiu-Ping; Chen, Tzy-Ling; Chiu, Li-An; Lee, San-Liang; Wang, An-Bang

    2012-01-01

    The study presented here explored a student evaluation of the teaching effectiveness of a nationwide innovative education program on image display technology in Taiwan. Using survey data collected through an online questionnaire system, covering 165 classes across 30 colleges and universities in Taiwan, the study aimed to understand the teaching…

  1. A Therapeutic Program To Improve the Body Image of Pre-Puberty Exceptional Education Girls.

    ERIC Educational Resources Information Center

    Pfeiffer, Linda Jo

    This practicum designed and implemented a body image improvement program for seven severely emotionally disturbed latency-aged girls. Students participated once per week for 12 weeks in individual art therapy and in group sessions. The practicum incorporated therapeutic art activities with verbal interactions and utilized human growth films,…

  2. FOXO target gene CTDSP2 regulates cell cycle progression through Ras and p21Cip1/Waf1

    PubMed Central

    Kloet, David E.A.; Polderman, Paulien E.; Eijkelenboom, Astrid; Smits, Lydia M.; vanTriest, Miranda H.; vandenBerg, Maaike C.W.; Koerkamp, Marian J. Groot; vanLeenen, Dik; Lijnzaad, Philip; Holstege, Frank C.; Burgering, Boudewijn M.T.

    2015-01-01

    Activity of FOXO (forkhead box O) transcription factors is inhibited by growth factor–PI3K (phosphoinositide 3-kinase)–PKB (protein kinase B)/Akt signalling to control a variety of cellular processes including cell cycle progression. Through comparative analysis of a number of microarray datasets we identified a set of genes commonly regulated by FOXO proteins and PI3K–PKB/Akt, which includes CTDSP2 (C-terminal domain small phosphatase 2). We validated CTDSP2 as a genuine FOXO target gene and show that ectopic CTDSP2 can induce cell cycle arrest. We analysed transcriptional regulation after CTDSP2 expression and identified extensive regulation of genes involved in cell cycle progression, which depends on the phosphatase activity of CTDSP2. The most notably regulated gene is the CDK (cyclin-dependent kinase) inhibitor p21Cip1/Waf1 and in the present study we show that p21Cip1/Waf1 is partially responsible for the cell cycle arrest through decreasing cyclin–CDK activity. Our data suggest that CTDSP2 induces p21Cip1/Waf1 through increasing the activity of Ras. As has been described previously, Ras induces p21Cip1/Waf1 through p53-dependent and p53-independent pathways and indeed both p53 and MEK inhibition can mitigate the CTDSP2-induced p21Cip1/Waf1 mRNA up-regulation. In support of Ras activation by CTDSP2, depletion of endogenous CTDSP2 results in reduced Ras activity and thus CTDSP2 seems to be part of a larger set of genes regulated by FOXO proteins, which increase growth factor signalling upon FOXO activation. PMID:25990325

  3. Multi-Objective Genetic Programming with Redundancy-Regulations for Automatic Construction of Image Feature Extractors

    NASA Astrophysics Data System (ADS)

    Watchareeruetai, Ukrit; Matsumoto, Tetsuya; Takeuchi, Yoshinori; Kudo, Hiroaki; Ohnishi, Noboru

    We propose a new multi-objective genetic programming (MOGP) for automatic construction of image feature extraction programs (FEPs). The proposed method was originated from a well known multi-objective evolutionary algorithm (MOEA), i.e., NSGA-II. The key differences are that redundancy-regulation mechanisms are applied in three main processes of the MOGP, i.e., population truncation, sampling, and offspring generation, to improve population diversity as well as convergence rate. Experimental results indicate that the proposed MOGP-based FEP construction system outperforms the two conventional MOEAs (i.e., NSGA-II and SPEA2) for a test problem. Moreover, we compared the programs constructed by the proposed MOGP with four human-designed object recognition programs. The results show that the constructed programs are better than two human-designed methods and are comparable with the other two human-designed methods for the test problem.

  4. Report on the Imaging Workshop for the Genomes to Life Program, April 16-18, 2002

    SciTech Connect

    Colson, STEVEN

    2003-08-04

    This report is a result of the Imaging Workshop for the Genomes to Life (GTL) program held April 16-19, 2002, in Charlotte, North Carolina. The meeting was sponsored by the Office of Biological and Environmental Research and the Office of Advanced Scientific Computing Research of the U.S. Department of Energy's (DOE) Office of Science. The purpose of the workshop was to project a broad vision for future needs and determine the value of imaging to GTL program research. The workshop included four technical sessions with plenary lectures on biology and technology perspectives and technical presentations on needs and approaches as they related to the following areas of the GTL program: (1) Molecular machines (protein complexes); (2) Intracellular and cellular structure, function, and processes; (3) Multicellular: Monoclonal and heterogeneous multicellular systems, cell-cell signaling, and model systems; and (4) Cells in situ and in vivo: Bacteria in the natural environment, microenvironment, and in vivo systems.

  5. Smiles: a fortran-77 program for sequential machine interpreted lineament extraction using digital images

    NASA Astrophysics Data System (ADS)

    Raghavan, Venkatesh; Wadatsumu, Kiyoshi; Masumoto, Shinji

    1994-03-01

    A FORTRAN-77 program Sequential Machine Interpreted Lineament Extraction System (SMILES) is presented, which is useful for automatic and manual extraction of lineament information from digital images. The SMILES is a stand-alone package composed of several modules which perform the function of image display, lineament information extraction, data management, output generation, and preliminary analysis. The program architecture and application results are described. The program has been tested using LANDSAT MSS data of southwestern Japan. The Directional Segment Detection Algorithm (DSDA) also has been applied to shaded relief maps generated from digital elevation data of the same area. Interpretation of aerial photograph stereo pairs reveals that the machine interpreted features show photogeological expressions that are characteristic of geologic lineaments.

  6. Magnetic resonance imaging-guided brachytherapy for cervical cancer: initiating a program

    PubMed Central

    Prisciandaro, Joann I.; Soliman, Abraam; Ravi, Ananth; Song, William Y.

    2015-01-01

    Over the past decade, the application of magnetic resonance imaging (MRI) has increased, and there is growing evidence to suggest that improvements in accuracy of target delineation in MRI-guided brachytherapy may improve clinical outcomes in cervical cancer. To implement a high quality image guided brachytherapy program, a multidisciplinary team is required with appropriate expertise as well as an adequate patient load to ensure a sustainable program. It is imperative to know that the most important source of uncertainty in the treatment process is related to target delineation and therefore, the necessity of training and expertise as well as quality assurance should be emphasized. A short review of concepts and techniques that have been developed for implementation and/or improvement of workflow of a MRI-guided brachytherapy program are provided in this document, so that institutions can use and optimize some of them based on their resources to minimize their procedure times. PMID:26622249

  7. Magnetic resonance imaging-guided brachytherapy for cervical cancer: initiating a program.

    PubMed

    Owrangi, Amir M; Prisciandaro, Joann I; Soliman, Abraam; Ravi, Ananth; Song, William Y

    2015-10-01

    Over the past decade, the application of magnetic resonance imaging (MRI) has increased, and there is growing evidence to suggest that improvements in accuracy of target delineation in MRI-guided brachytherapy may improve clinical outcomes in cervical cancer. To implement a high quality image guided brachytherapy program, a multidisciplinary team is required with appropriate expertise as well as an adequate patient load to ensure a sustainable program. It is imperative to know that the most important source of uncertainty in the treatment process is related to target delineation and therefore, the necessity of training and expertise as well as quality assurance should be emphasized. A short review of concepts and techniques that have been developed for implementation and/or improvement of workflow of a MRI-guided brachytherapy program are provided in this document, so that institutions can use and optimize some of them based on their resources to minimize their procedure times. PMID:26622249

  8. Prognostic implications of p21 (Waf1/Cip1) immunolocalization in multiple myeloma.

    PubMed

    Ohata, Masahiko; Nakamura, Shinobu; Fujita, Hiroyuki; Isemura, Mamoru

    2005-06-01

    Protein p21 (Waf1/Cip1) plays a critical role in controlling the cell cycle especially as a check point of G1 and is intimately associated with important cellular activities including differentiation, senescence, tumorigenesis, and apoptosis. In the present study, we examined the expression of p21 in multiple myeloma (MM) cells for prognostic evaluation. The immunocytochemical localization of p21 could be categorized into nuclear and cytoplasmic types. The nuclear-type p21 localization was correlated with the severity of MM and the expression of proliferating cell nuclear antigen and p53. Patients with the nuclear-type p21 localization survived significantly shorter than those with the cytoplasmic-type localization. Thus, the present study suggests that p21-immunolocalization can be a useful prognostic marker of MM. PMID:16011301

  9. CIP spin torque effect in the spin valve pinned with an oxide antiferromagnetic layer

    NASA Astrophysics Data System (ADS)

    Thuan, Nguyen Chi; Van Dai, Nguyen; Nguyen Hoai Nam, Dao; Phuc, Nguyen Xuan; Van Hong, Le

    2009-09-01

    Spin valve Ni0.85Co0.15O/Co85Fe15/Cu/Co85Fe15 was manufactured by a RF magnetron sputtering system. M(H) and R(H) characteristics of the spin valve were measured in CIP configuration at room temperature has a magnetoresistance ratio of about 8% and a high exchange bias at room temperature. The current density and angle between the applied magnetic field and injection current were changed in an aim to observe their effects on MR and exchange bias of the spin valve. The current density and angle strongly affect MR and exchange bias. Both MR and exchange bias clearly decrease in dependence of the current density and direction of the magnetic field. It is supposed to be related with a current-induced spin torque in device.

  10. Aerial image simulation for partial coherent system with programming development in MATLAB

    NASA Astrophysics Data System (ADS)

    Hasan, Md. Nazmul; Rahman, Md. Momtazur; Udoy, Ariful Banna

    2014-10-01

    Aerial image can be calculated by either Abbe's method or sum of coherent system decomposition (SOCS) method for partial coherent system. This paper introduces a programming with Matlab code that changes the analytical representation of Abbe's method to the matrix form, which has advantages for both Abbe's method and SOCS since matrix calculation is easier than double integration over object plane or pupil plane. First a singular matrix P is derived from a pupil function and effective light source in the spatial frequency domain. By applying Singular Value Decomposition (SVD) to the matrix P, eigenvalues and eigenfunctions are obtained. The aerial image can then be computed by the eigenvalues and eigenfunctions without calculation of Transmission Cross Coefficient (TCC). The aerial final image is almost identical as an original cross mask and the intensity distribution on image plane shows that it is almost uniform across the linewidth of the mask.

  11. MRO's High Resolution Imaging Science Experiment (HiRISE) Education And Public Outreach program

    NASA Astrophysics Data System (ADS)

    Gulick, V. C.; Davatzes, A.; McEwen, A.

    2006-12-01

    HiRISE provides an innovative education and public outreach program with a variety of formal and informal educational activities. The centerpiece of HiRISE's E/PO program is it's interactive website called HiWeb (http://marsoweb.nasa.nasa.gov/hirise and http://hirise.lpl.arizona.edu). HiWeb provides an image suggestion facility where the public can submit suggestions for HiRISE images and view HiRISE images in context with other available Mars data. HiRISE EPO has developed K-14 educational materials including activity, coloring and comic books that focus on Mars geology, the image suggestion process, understanding the HiRISE camera and working with digital image data. In addition, we have developed interactive educational games including Mars crosswords, jigsaws, word searches, and flash cards to provide fun ways for students to learn more about Mars. All educational materials and games are aligned with the National Science Standards. HiRISE Clickworkers will provide online opportunities for the public to assist the team in creating geologic feature databases (gullies, boulders, craters, wind streaks, etc.) present in the HiRISE images in addition to other innovative opportunities. Web events (including web chats, casts and forums) with HiRISE team members, will help guide students and educators of HiRISE capabilities and science goals and provide support for submitting good image suggestions. Educator workshops will be held each year at or near the institution of HiRISE team members. Workshop support materials and instructions for all hands-on activities will be placed on HiWeb to facilitate sharing of information with other educators and the general public. Large-scale displays of HiRISE images will be available at several at museums and planetariums.

  12. Disparity Map Generation from Illumination Variant Stereo Images Using Efficient Hierarchical Dynamic Programming

    PubMed Central

    Borisagar, Viral H.; Zaveri, Mukesh A.

    2014-01-01

    A novel hierarchical stereo matching algorithm is presented which gives disparity map as output from illumination variant stereo pair. Illumination difference between two stereo images can lead to undesirable output. Stereo image pair often experience illumination variations due to many factors like real and practical situation, spatially and temporally separated camera positions, environmental illumination fluctuation, and the change in the strength or position of the light sources. Window matching and dynamic programming techniques are employed for disparity map estimation. Good quality disparity map is obtained with the optimized path. Homomorphic filtering is used as a preprocessing step to lessen illumination variation between the stereo images. Anisotropic diffusion is used to refine disparity map to give high quality disparity map as a final output. The robust performance of the proposed approach is suitable for real life circumstances where there will be always illumination variation between the images. The matching is carried out in a sequence of images representing the same scene, however in different resolutions. The hierarchical approach adopted decreases the computation time of the stereo matching problem. This algorithm can be helpful in applications like robot navigation, extraction of information from aerial surveys, 3D scene reconstruction, and military and security applications. Similarity measure SAD is often sensitive to illumination variation. It produces unacceptable disparity map results for illumination variant left and right images. Experimental results show that our proposed algorithm produces quality disparity maps for both wide range of illumination variant and invariant stereo image pair. PMID:25386604

  13. Catalytic Molecular Imaging of MicroRNA in Living Cells by DNA-Programmed Nanoparticle Disassembly.

    PubMed

    He, Xuewen; Zeng, Tao; Li, Zhi; Wang, Ganglin; Ma, Nan

    2016-02-24

    Molecular imaging is an essential tool for disease diagnostics and treatment. Direct imaging of low-abundance nucleic acids in living cells remains challenging because of the relatively low sensitivity and insufficient signal-to-background ratio of conventional molecular imaging probes. Herein, we report a class of DNA-templated gold nanoparticle (GNP)-quantum dot (QD) assembly-based probes for catalytic imaging of cancer-related microRNAs (miRNA) in living cells with signal amplification capacity. We show that a single miRNA molecule could catalyze the disassembly of multiple QDs with the GNP through a DNA-programmed thermodynamically driven entropy gain process, yielding significantly amplified QD photoluminescence (PL) for miRNA imaging. By combining the robust PL of QDs with the catalytic amplification strategy, three orders of magnitude improvement in detection sensitivity is achieved in comparison with non-catalytic imaging probe, which enables facile and accurate differentiation between cancer cells and normal cells by miRNA imaging in living cells. PMID:26694689

  14. What works in secondary schools? A systematic review of classroom-based body image programs.

    PubMed

    Yager, Zali; Diedrichs, Phillippa C; Ricciardelli, Lina A; Halliwell, Emma

    2013-06-01

    Governments, schools, and curriculum authorities are increasingly recognizing that body image during adolescence is a public health issue that warrants attention in the school setting. After 30 years of eating disorder prevention research, and given the current interest in this area, it seems timely to review the research on interventions to improve body image in schools. We reviewed universal-selective, classroom-based programs that have been conducted since the year 2000, among adolescents, and found 16 eligible intervention programs. Seven of these programs were effective in improving body image on at least one measure, from pre to post test, though effect sizes were small (d=0.22-0.48). These effective programs were conducted among younger adolescents 12.33-13.62 years, and included activities focusing on media literacy, self esteem, and the influence of peers. Implications for school personnel and curriculum authorities are discussed, and we provide recommendations for a strategic approach to future research in this area. PMID:23683611

  15. Probabilistic person identification in TV news programs using image web database

    NASA Astrophysics Data System (ADS)

    Battisti, F.; Carli, M.; Leo, M.; Neri, A.

    2014-02-01

    The automatic labeling of faces in TV broadcasting is still a challenging problem. The high variability in view points, facial expressions, general appearance, and lighting conditions, as well as occlusions, rapid shot changes, and camera motions, produce significant variations in image appearance. The application of automatic tools for face recognition is not yet fully established and the human intervention is needed. In this paper, we deal with the automatic face recognition in TV broadcasting programs. The target of the proposed method is to identify the presence of a specific person in a video by means of a set of images downloaded from Web using a specific search key.

  16. CIP10 optimization for 4,4-methylene diphenyl diisocyanate aerosol sampling and field comparison with impinger method.

    PubMed

    Puscasu, Silvia; Aubin, Simon; Cloutier, Yves; Sarazin, Philippe; Tra, Huu V; Gagné, Sébastien

    2015-04-01

    4,4-methylene diphenyl diisocyanate (MDI) aerosol exposure evaluation in spray foam insulation application is known as being a challenge because the spray foam application actually involves a fast-curing process. Available techniques are either not user-friendly or are inaccurate or not validated for this application. To address these issues, a new approach using a CIP10M was developed to appropriately collect MDI aerosol in spray foam insulation while being suitable for personal sampling. The CIP10M is a commercially available personal aerosol sampler that has been validated for the collection of microbial spores into a liquid medium. Tributylphosphate with 1-(2-methoxyphenyl)piperazine (MOPIP) was introduced into the CIP10M to collect and stabilize the MDI aerosols. The limit of detection and limit of quantification of the method were 0.007 and 0.024 μg ml(-1), respectively. The dynamic range was from 0.024 to 0.787 μg ml(-1) (with R (2) ≥ 0.990), which corresponds to concentrations in the air from 0.04 to 1.3 µg m(-3), assuming 60 min of sampling at 10 l min(-1). The intraday and interday analytical precisions were <2% for all of the concentration levels tested, and the accuracy was within an appropriate range of 98 ± 1%. No matrix effect was observed, and a total recovery of 99% was obtained. Parallel sampling was performed in a real MDI foam spraying environment with a CIP10M and impingers containing toluene/MOPIP (reference method). The results obtained show that the CIP10M provides levels of MDI monomer in the same range as the impingers, and higher levels of MDI oligomers. The negative bias observed for MDI monomer was between 2 and 26%, whereas the positive bias observed for MDI oligomers was between 76 and 113%, with both biases calculated with a confidence level of 95%. The CIP10M seems to be a promising approach for MDI aerosol exposure evaluation in spray foam applications. PMID:25452291

  17. Model-integrated program synthesis environment for parallel/real-time image processing

    NASA Astrophysics Data System (ADS)

    Moore, Michael S.; Sztipanovitz, Janos; Karsai, Gabor; Nichols, James A.

    1997-09-01

    In this paper, it is shown that, through the use of model- integrated program synthesis (MIPS), parallel real-time implementations of image processing data flows can be synthesized from high level graphical specifications. The complex details in inherent to parallel and real-time software development become transparent to the programmer, enabling the cost-effective exploitation of parallel hardware for building more flexible and powerful real-time imaging systems. The model integrated real-time image processing system (MIRTIS) is presented as an example. MIRTIS employs the multigraph architecture (MGA), a framework and set of tools for building MIPS systems, to generate parallel real-time image processing software which runs under the control of a parallel run-time kernel on a network of Texas Instruments TMS320C40 DSPs (C40s). The MIRTIS models contain graphical declarations of the image processing computations to be performed, the available hardware resources, and the timing constraints of the application. The MIRTIS model interpreter performs the parallelization, scaling, and mapping of the computations to the resources automatically or determines that the timing constraints cannot be met with the available resources. MIRTIS is a clear example of how parallel real-time image processing systems can be built which are (1) cost-effectively programmable, (2) flexible, (3) scalable, and (4) built from commercial off-the-shelf (COTS) components.

  18. An algorithm and program for data processing from a Compton scattering imaging device

    NASA Astrophysics Data System (ADS)

    Vasiliev, V. N.; Zaytseva, K. V.

    2005-07-01

    The VolumeScope, a prototype 3D X-ray scanner based on Compton backscatter detection, was designed for examination of a human body electron density distribution. An algorithm and computer program for 3D image reconstruction from the VolumeScope measured data are presented. The reconstruction includes corrections for photon attenuation and multiple scatter in surrounding tissues and postprocessing digital filtering. Properties of multiple scattered photons inside the object of examination were studied by Monte Carlo technique and a geometrical efficiency of the multiple scatter detection was calculated on the base of the collimator design. The contribution of multiple scattered photons in semi-infinite water medium was from 15 to 23% of maximum detector response. The VolumeScope program is described to perform data processing and display the electron density distribution of the object as 2D grayscale images and 3D surfaces of internal structures.

  19. Development of an AutoFlat program for the acquisition of effective flat images in the automated observation system

    NASA Astrophysics Data System (ADS)

    Yoon, Joh-Na; Kim, Yonggi; Kim, Dong-Heun; Yim, Hong-Suh

    2013-12-01

    The purpose of this study is to develop an observation program for obtaining effective flat images that are necessary for photometric observation. The development of the program was achieved by improving the existing method for obtaining twilight flat images. The existing method for obtaining twilight flat images acquires flat images by observing the sky light after sunset or light before sunrise. The decision of when to observe flat images at each night is solely dependent on the judgment of an observer, and thus the obtained flat images for particular nights may not be clean. Especially, in the case of the observatories where an automated observation system is in operation, there is a difficulty that an observer should pay attention during sunrise and sunset in order to obtain flat images. In this study, a computer program is developed to improve this inconvenience and to efficiently perform photometric observation in the observatories where an automated observation system is applied. This program can obtain flat images by calculating the time for obtaining flat images automatically and the exposure time using a numerically calculated function. When obtaining twilight flat images at dusk and at dawn, the developed program performs automated observation and provides effective flat images by acquiring appropriate exposure time considering the sunrise and sunset times that vary depending on the day of observation. The code for performing this task was added to Obs Tool II (Yoon et al. 2006), which is the automated observation system of the Chungbuk National University Observatory, and the usefulness of the developed program was examined by performing an actual automated observation. If this program is applied to other observatories where automated observation is in operation, it is expected that stable and highquality flat images could be obtained, which can be used for the pre-processing of photometric observation data.

  20. How International Students Select Offshore Programs: The Influence of Image, Attitude, Subject Norm, and Perceived Behavioral Control

    ERIC Educational Resources Information Center

    Li, Jianyao; Liu, Fang; Rojas-Méndez, José I.

    2013-01-01

    Previous research studies identified country image as an important variable in international students' selection of onshore programs, and it is often perceived that there is little difference between onshore and offshore program selection. Looking at a sample of high school students in China and their selections of offshore programs (from a…

  1. Overexpression of CIP2A in clear cell renal cell carcinoma promotes cellular epithelial-mesenchymal transition and is associated with poor prognosis.

    PubMed

    Tang, Qizhen; Wang, Qifei; Zeng, Guang; Li, Quanlin; Jiang, Tao; Zhang, Zhiwei; Zheng, Wei; Wang, Kenan

    2015-11-01

    Cancerous inhibitor of protein phosphatase 2A (CIP2A) is a newly characterized oncoprotein involved in a variety of malignant tumors. However, its expression pattern and biological functions in clear cell renal cell carcinoma (ccRCC) remain unclear. In the present study, our findings demonstrated that expressions of CIP2A mRNA and protein in ccRCC tissues and cell lines were significantly higher than those in paired normal renal tissues or normal renal tubular epithelial cells (P<0.05). High CIP2A level was closely correlated with T stage (P=0.001), tumor size (P=0.009), lymph node metastasis (P=0.014), vascular invasion (P=0.018) and high Snail expression (P<0.001). Additionally, ccRCC patients with high CIP2A expression had significantly shorter overall survival (OS, P<0.001) and disease-free survival (DFS, P<0.001) when compared with patients with the low expression of CIP2A. On Cox multivariate analysis, CIP2A overexpression was an independent and significant prognostic factor for OS (P=0.010) and DFS (P=0.004). Furthermore, knockdown of the CIP2A expression significantly reduced ccRCC cell invasion, with decreased Snail and Vimentin expression, and increased E-cadherin expression. Taken together, our data identified CIP2A as a critical oncoprotein involved in cell invasion and epithelial mesenchymal transition (EMT), which could serve as a therapeutic target in ccRCC. PMID:26327467

  2. Image subsampling and point scoring approaches for large-scale marine benthic monitoring programs

    NASA Astrophysics Data System (ADS)

    Perkins, Nicholas R.; Foster, Scott D.; Hill, Nicole A.; Barrett, Neville S.

    2016-07-01

    Benthic imagery is an effective tool for quantitative description of ecologically and economically important benthic habitats and biota. The recent development of autonomous underwater vehicles (AUVs) allows surveying of spatial scales that were previously unfeasible. However, an AUV collects a large number of images, the scoring of which is time and labour intensive. There is a need to optimise the way that subsamples of imagery are chosen and scored to gain meaningful inferences for ecological monitoring studies. We examine the trade-off between the number of images selected within transects and the number of random points scored within images on the percent cover of target biota, the typical output of such monitoring programs. We also investigate the efficacy of various image selection approaches, such as systematic or random, on the bias and precision of cover estimates. We use simulated biotas that have varying size, abundance and distributional patterns. We find that a relatively small sampling effort is required to minimise bias. An increased precision for groups that are likely to be the focus of monitoring programs is best gained through increasing the number of images sampled rather than the number of points scored within images. For rare species, sampling using point count approaches is unlikely to provide sufficient precision, and alternative sampling approaches may need to be employed. The approach by which images are selected (simple random sampling, regularly spaced etc.) had no discernible effect on mean and variance estimates, regardless of the distributional pattern of biota. Field validation of our findings is provided through Monte Carlo resampling analysis of a previously scored benthic survey from temperate waters. We show that point count sampling approaches are capable of providing relatively precise cover estimates for candidate groups that are not overly rare. The amount of sampling required, in terms of both the number of images and

  3. Automatic electrode configuration selection for image-guided cochlear implant programming

    NASA Astrophysics Data System (ADS)

    Zhao, Yiyuan; Dawant, Benoit M.; Noble, Jack H.

    2015-03-01

    Cochlear implants (CIs) are neural prosthetics that stimulate the auditory nerve pathways within the cochlea using an implanted electrode array to restore hearing. After implantation, the CI is programmed by an audiologist who determines which electrodes are active, i.e., the electrode configuration, and selects other stimulation settings. Recent clinical studies by our group have shown that hearing outcomes can be significantly improved by using an image-guided electrode configuration selection technique we have designed. Our goal in this work is to automate the electrode configuration selection step with the long term goal of developing a fully automatic system that can be translated to the clinic. Until now, the electrode configuration selection step has been performed by an expert with the assistance of image analysis-based estimates of the electrode-neural interface. To automatically determine the electrode configuration, we have designed an optimization approach and propose the use of a cost function with feature terms designed to interpret the image analysis data in a similar fashion as the expert. Further, we have designed an approach to select parameters in the cost function using our database of existing electrode configuration plans as training data. The results we present show that our automatic approach results in electrode configurations that are better or equally as good as manually selected configurations in over 80% of the cases tested. This method represents a crucial step towards clinical translation of our image-guided cochlear implant programming system.

  4. Comparison of two imaging programs in predicting the soft tissue changes with mandibular advancement surgery.

    PubMed

    Ravindranath, Sneha; Krishnaswamy, Nathamuni Rengarajan; Sundaram, Venkateswaran

    2011-01-01

    Establishing common objectives and expectations concerning the outcome of proposed surgical orthodontic therapy is a crucial part of the treatment planning process, which has been greatly simplified by imaging software. The purpose of this study was to investigate the reliability of two surgical imaging programs--Dolphin Imaging 10 and Vistadent OC--in simulating the actual outcome of mandibular advancement surgery by using a visual analog scale (VAS) judged by a panel of orthodontists, oral surgeons, and laypersons. The predictions were also analyzed with soft tissue cephalometric evaluation. The results of the study showed that in predicting the surgical outcome evaluated by the VAS, both programs received a mean rating of fair. One was marginally superior for the overall assessment among all three panelist groups. Region-wise, rating indicated the lower lip region to be the least accurate, and the submental region received the highest scores. The soft tissue cephalometric parameters showed minimal differences except for the lower lip parameters. Thus, Dolphin Imaging 10 and Vistadent OC are reliable in predicting mandibular advancement surgical outcomes with inaccuracies chiefly in the lower lip region. PMID:22299108

  5. High CIP2A levels correlate with an antiapoptotic phenotype that can be overcome by targeting BCL-XL in chronic myeloid leukemia.

    PubMed

    Lucas, C M; Milani, M; Butterworth, M; Carmell, N; Scott, L J; Clark, R E; Cohen, G M; Varadarajan, S

    2016-06-01

    Cancerous inhibitor of protein phosphatase 2A (CIP2A) is a predictive biomarker of disease progression in many malignancies, including imatinib-treated chronic myeloid leukemia (CML). Although high CIP2A levels correlate with disease progression in CML, the underlying molecular mechanisms remain elusive. In a screen of diagnostic chronic phase samples from patients with high and low CIP2A protein levels, high CIP2A levels correlate with an antiapoptotic phenotype, characterized by downregulation of proapoptotic BCL-2 family members, including BIM, PUMA and HRK, and upregulation of the antiapoptotic protein BCL-XL. These results suggest that the poor prognosis of patients with high CIP2A levels is due to an antiapoptotic phenotype. Disrupting this antiapoptotic phenotype by inhibition of BCL-XL via RNA interference or A-1331852, a novel, potent and BCL-XL-selective inhibitor, resulted in extensive apoptosis either alone or in combination with imatinib, dasatinib or nilotinib, both in cell lines and in primary CD34(+) cells from patients with high levels of CIP2A. These results demonstrate that BCL-XL is the major antiapoptotic survival protein and may be a novel therapeutic target in CML. PMID:26987906

  6. Novel role of cortactin in G protein-coupled receptor agonist-induced nuclear export and degradation of p21Cip1.

    PubMed

    Janjanam, Jagadeesh; Rao, Gadiparthi N

    2016-01-01

    Monocyte chemotactic protein 1 (MCP1) stimulates phosphorylation of cortactin on Y421 and Y446 residues in a time-dependent manner and phosphorylation at Y446 but not Y421 residue is required for MCP1-induced CDK-interacting protein 1 (p21Cip1) nuclear export and degradation in facilitating human aortic smooth muscle cell (HASMC) proliferation. In addition, MCP1-induced cortactin tyrosine phosphorylation, p21Cip1 degradation and HASMC proliferation are dependent on Fyn activation. Upstream to Fyn, MCP1 stimulated C-C chemokine receptor type 2 (CCR2) and Gi/o and inhibition of either one of these molecules using their specific antagonists or inhibitors attenuated MCP1-induced cortactin tyrosine phosphorylation, p21Cip1 degradation and HASMC proliferation. Cortactin phosphorylation at Y446 residue is also required for another G protein-coupled receptor (GPCR) agonist, thrombin-induced p21Cip1 nuclear export and its degradation in promoting HASMC proliferation. Quite interestingly, the receptor tyrosine kinase (RTK) agonist, platelet-derived growth factor-BB (PDGF-BB)-induced p21Cip1 degradation and HASMC proliferation do not require cortactin tyrosine phosphorylation. Together, these findings demonstrate that tyrosine phosphorylation of cortactin at Y446 residue is selective for only GPCR but not RTK agonist-induced nuclear export and proteolytic degradation of p21Cip1 in HASMC proliferation. PMID:27363897

  7. High CIP2A levels correlate with an antiapoptotic phenotype that can be overcome by targeting BCL-XL in chronic myeloid leukemia

    PubMed Central

    Lucas, C M; Milani, M; Butterworth, M; Carmell, N; Scott, L J; Clark, R E; Cohen, G M; Varadarajan, S

    2016-01-01

    Cancerous inhibitor of protein phosphatase 2A (CIP2A) is a predictive biomarker of disease progression in many malignancies, including imatinib-treated chronic myeloid leukemia (CML). Although high CIP2A levels correlate with disease progression in CML, the underlying molecular mechanisms remain elusive. In a screen of diagnostic chronic phase samples from patients with high and low CIP2A protein levels, high CIP2A levels correlate with an antiapoptotic phenotype, characterized by downregulation of proapoptotic BCL-2 family members, including BIM, PUMA and HRK, and upregulation of the antiapoptotic protein BCL-XL. These results suggest that the poor prognosis of patients with high CIP2A levels is due to an antiapoptotic phenotype. Disrupting this antiapoptotic phenotype by inhibition of BCL-XL via RNA interference or A-1331852, a novel, potent and BCL-XL-selective inhibitor, resulted in extensive apoptosis either alone or in combination with imatinib, dasatinib or nilotinib, both in cell lines and in primary CD34+ cells from patients with high levels of CIP2A. These results demonstrate that BCL-XL is the major antiapoptotic survival protein and may be a novel therapeutic target in CML. PMID:26987906

  8. Cucurbitacin B reverses multidrug resistance by targeting CIP2A to reactivate protein phosphatase 2A in MCF-7/adriamycin cells.

    PubMed

    Cai, Fen; Zhang, Liang; Xiao, Xiangling; Duan, Chao; Huang, Qiuyue; Fan, Chunsheng; Li, Jian; Liu, Xuewen; Li, Shan; Liu, Ying

    2016-08-01

    Cancerous inhibitor of protein phosphatase 2A (CIP2A) is a human oncoprotein that is overexpressed in various tumors. A previous study found that CIP2A expression is associated with doxorubicin (Dox) resistance. In the present study, we investigated whether cucurbitacin B (CuB), a natural anticancer compound found in Cucurbitaceae, reversed multidrug resistance (MDR) and downregulated CIP2A expression in MCF-7/Adriamycin (MCF-7/Adr) cells, a human breast multidrug-resistant cancer cell line. CuB treatment significantly suppressed MCF-7/Adr cell proliferation, and reversed Dox resistance. CuB treatment also induced caspase-dependent apoptosis, decreased phosphorylation of Akt (pAkt). The suppression of pAkt was mediated through CuB-induced activation of protein phosphatase 2A (PP2A). Furthermore, CuB activated PP2A through the suppression of CIP2A. Silencing CIP2A enhanced CuB-induced growth inhibition, apoptosis and MDR inhibition in MCF-7/Adr cells. In conclusion, we found that enhancement of PP2A activity by inhibition of CIP2A promotes the reversal of MDR induced by CuB. PMID:27350399

  9. Novel role of cortactin in G protein-coupled receptor agonist-induced nuclear export and degradation of p21Cip1

    PubMed Central

    Janjanam, Jagadeesh; Rao, Gadiparthi N.

    2016-01-01

    Monocyte chemotactic protein 1 (MCP1) stimulates phosphorylation of cortactin on Y421 and Y446 residues in a time-dependent manner and phosphorylation at Y446 but not Y421 residue is required for MCP1-induced CDK-interacting protein 1 (p21Cip1) nuclear export and degradation in facilitating human aortic smooth muscle cell (HASMC) proliferation. In addition, MCP1-induced cortactin tyrosine phosphorylation, p21Cip1 degradation and HASMC proliferation are dependent on Fyn activation. Upstream to Fyn, MCP1 stimulated C-C chemokine receptor type 2 (CCR2) and Gi/o and inhibition of either one of these molecules using their specific antagonists or inhibitors attenuated MCP1-induced cortactin tyrosine phosphorylation, p21Cip1 degradation and HASMC proliferation. Cortactin phosphorylation at Y446 residue is also required for another G protein-coupled receptor (GPCR) agonist, thrombin-induced p21Cip1 nuclear export and its degradation in promoting HASMC proliferation. Quite interestingly, the receptor tyrosine kinase (RTK) agonist, platelet-derived growth factor-BB (PDGF-BB)-induced p21Cip1 degradation and HASMC proliferation do not require cortactin tyrosine phosphorylation. Together, these findings demonstrate that tyrosine phosphorylation of cortactin at Y446 residue is selective for only GPCR but not RTK agonist-induced nuclear export and proteolytic degradation of p21Cip1 in HASMC proliferation. PMID:27363897

  10. A Lotus japonicus Cochaperone Protein Interacts With the Ubiquitin-Like Domain Protein CIP73 and Plays a Negative Regulatory Role in Nodulation.

    PubMed

    Kang, Heng; Xiao, Aifang; Huang, Xiaoqin; Gao, Xioumei; Yu, Haixiang; He, Xingxing; Zhu, Hui; Hong, Zonglie; Zhang, Zhongming

    2015-05-01

    The calcium/calmodulin-dependent protein kinase CCaMK forms a complex with its phosphorylation target CIP73 (CCaMK-interacting protein of 73 kDa). In this work, a homolog of the animal HSC/HSP70 interacting protein (HIP) was identified as an interacting partner of CIP73 in Lotus japonicus. L. japonicus HIP contains all functional domains characteristic of animal HIP proteins. The C-terminal STI1-like domain of L. japonicus HIP was found to be necessary and sufficient for interaction with CIP73. The interaction between CIP73 and HIP occurred in both the nuclei and cytoplasm in Nicotiana benthamiana leaf cells. The interactions between CIP73 and HIP and between CIP73 and CCaMK could take place simultaneously in the same nuclei. HIP transcripts were detected in all plant tissues tested. As nodule primordia developed into young nodules, the expression of HIP was down-regulated and the HIP transcript level became very low in mature nodules. More nodules were formed in transgenic hairy roots of L. japonicus expressing HIP RNA interference at 16 days postinoculation as compared with the control hairy roots expressing the empty vector. It appears that HIP may play a role as a negative regulator for nodulation. PMID:25761207

  11. Exploration of picture grammars, grammar learning, and inductive logic programming for image understanding

    NASA Astrophysics Data System (ADS)

    Ducksbury, P. G.; Kennedy, C.; Lock, Z.

    2003-09-01

    Grammars have been used for the formal specification of programming languages, and there are a number of commercial products which now use grammars. However, these have tended to be focused mainly on flow control type applications. In this paper, we consider the potential use of picture grammars and inductive logic programming in generic image understanding applications, such as object recognition. A number of issues are considered, such as what type of grammar needs to be used, how to construct the grammar with its associated attributes, difficulties encountered with parsing grammars followed by issues of automatically learning grammars using a genetic algorithm. The concept of inductive logic programming is then introduced as a method that can overcome some of the earlier difficulties.

  12. NPOESS Preparatory Project Validation Program for the Visible/Infrared Imager/Radiometer Suite

    NASA Astrophysics Data System (ADS)

    Kopp, T.; Arnone, R. A.; Hutchison, K.; Jackson, J. M.; Kilcoyne, H.; Plonski, M.; Reed, B.; Sei, A.; Starr, D.; Wong, E.; Privette, J. L.

    2009-12-01

    The National Polar-orbiting Operational Environmental Satellite System (NPOESS) Program, in partnership with National Aeronautical Space Administration (NASA), will launch the NPOESS Preparatory Project (NPP), a risk reduction and data continuity mission, prior to the first operational NPOESS launch. The NPOESS Program, in partnership with Northrop Grumman Aerospace Systems, will execute the NPP Calibration and Validation (Cal/Val) program to ensure the data products comply with the requirements of the sponsoring agencies. The Visible/Infrared Imager/Radiometer Suite will produce 22 EDRs, including Imagery, cloud, aerosol, ocean, and land products. The calibration and validation plans establish science and user community leadership and participation, and demonstrated, cost-effective Cal/Val approaches. This presentation will provide an overview of the NPP Cal/Val plans for the VIIRS environmental data products.

  13. A novel approach for automated shoreline extraction from remote sensing images using low level programming

    NASA Astrophysics Data System (ADS)

    Rigos, Anastasios; Vaiopoulos, Aristidis; Skianis, George; Tsekouras, George; Drakopoulos, Panos

    2015-04-01

    . Although simple, with minimal human interaction and low level programming, this method can provide precise coastlines with just one pixel resolution. Images covering more locations are currently under consideration.

  14. Targeting p35/Cdk5 Signalling via CIP-Peptide Promotes Angiogenesis in Hypoxia

    PubMed Central

    Bosutti, Alessandra; Qi, Jie; Pennucci, Roberta; Bolton, David; Matou, Sabine; Ali, Kamela; Tsai, Li-Huei; Krupinski, Jerzy; Petcu, Eugene B.; Montaner, Joan; Al Baradie, Raid; Caccuri, Francesca; Caruso, Arnaldo; Alessandri, Giulio; Kumar, Shant; Rodriguez, Cristina; Martinez-Gonzalez, Jose; Slevin, Mark

    2013-01-01

    Cyclin-dependent kinase-5 (Cdk5) is over-expressed in both neurons and microvessels in hypoxic regions of stroke tissue and has a significant pathological role following hyper-phosphorylation leading to calpain-induced cell death. Here, we have identified a critical role of Cdk5 in cytoskeleton/focal dynamics, wherein its activator, p35, redistributes along actin microfilaments of spreading cells co-localising with p(Tyr15)Cdk5, talin/integrin beta-1 at the lamellipodia in polarising cells. Cdk5 inhibition (roscovitine) resulted in actin-cytoskeleton disorganisation, prevention of protein co-localization and inhibition of movement. Cells expressing Cdk5 (D144N) kinase mutant, were unable to spread, migrate and form tube-like structures or sprouts, while Cdk5 wild-type over-expression showed enhanced motility and angiogenesis in vitro, which was maintained during hypoxia. Gene microarray studies demonstrated myocyte enhancer factor (MEF2C) as a substrate for Cdk5-mediated angiogenesis in vitro. MEF2C showed nuclear co-immunoprecipitation with Cdk5 and almost complete inhibition of differentiation and sprout formation following siRNA knock-down. In hypoxia, insertion of Cdk5/p25-inhibitory peptide (CIP) vector preserved and enhanced in vitro angiogenesis. These results demonstrate the existence of critical and complementary signalling pathways through Cdk5 and p35, and through which coordination is a required factor for successful angiogenesis in sustained hypoxic condition. PMID:24098701

  15. p21WAF1/CIP1 Expression is Differentially Regulated by Metformin and Rapamycin

    PubMed Central

    Molnar, Zoltan; Millward, Ann B.; Tse, Wai; Demaine, Andrew G.

    2014-01-01

    The mammalian target of rapamycin (mTOR) pathway plays an important role in the development of diabetic nephropathy and other age-related diseases. One of the features of DN is the elevated expression of p21WAF1/CIP1. However, the importance of the mTOR signalling pathway in p21 regulation is poorly understood. Here we investigated the effect of metformin and rapamycin on mTOR-related phenotypes in cell lines of epithelial origin. This study reports that metformin inhibits high glucose-induced p21 expression. High glucose opposed metformin in regulating cell size, proliferation, and protein synthesis. These effects were associated with reduced AMPK activation, affecting downstream mTOR signalling. However, the inhibition of the mTOR pathway by rapamycin did not have a negative effect on p21 expression, suggesting that metformin regulates p21 upstream of mTOR. These findings provide support for the hypothesis that AMPK activation may regulate p21 expression, which may have implications for diabetic nephropathy and other age-related pathologies. PMID:26464852

  16. Algorithms and programming tools for image processing on the MPP:3

    NASA Technical Reports Server (NTRS)

    Reeves, Anthony P.

    1987-01-01

    This is the third and final report on the work done for NASA Grant 5-403 on Algorithms and Programming Tools for Image Processing on the MPP:3. All the work done for this grant is summarized in the introduction. Work done since August 1986 is reported in detail. Research for this grant falls under the following headings: (1) fundamental algorithms for the MPP; (2) programming utilities for the MPP; (3) the Parallel Pascal Development System; and (4) performance analysis. In this report, the results of two efforts are reported: region growing, and performance analysis of important characteristic algorithms. In each case, timing results from MPP implementations are included. A paper is included in which parallel algorithms for region growing on the MPP is discussed. These algorithms permit different sized regions to be merged in parallel. Details on the implementation and peformance of several important MPP algorithms are given. These include a number of standard permutations, the FFT, convolution, arbitrary data mappings, image warping, and pyramid operations, all of which have been implemented on the MPP. The permutation and image warping functions have been included in the standard development system library.

  17. Object-oriented programming approach to CCD data acquisition and image processing

    NASA Astrophysics Data System (ADS)

    Naidu, B. Nagaraja; Srinivasan, R.; Shankar, S. Murali

    1997-10-01

    In the recent past both the CCD camera controller hardware and software have witnessed a dynamic change to keep pace with the astronomer's imaging requirements. Conventional data acquisition software is based on menu driven programs developed using structured high level languages in non-window environment. An application under windows offers several advantages to the users, over the non-window approach, like multitasking, accessing large memory and inter-application communication. Windows also provides many programming facilities to the developers such as device-independent graphics, support to wide range of input/output devices, menus, icons, bitmaps. However, programming for windows environment under structured programming demands an in-depth knowledge of events, formats, handles and inner workings. Object-oriented approach simplifies the task of programming for windows by using object windows which manage the message- processing behavior and insulate the developer from the details of inner workings of windows. As a result, a window application can be developed in much less time and effort compared to conventional approaches. We have designed and developed an easy-to-use CCD data acquisition and processing software under Microsoft Windows 3.1 operating environment using object-Pascal for windows. The acquisition software exploits the advantages of the objects to provide custom specific tool boxes to implement different functions of CCD data accusation and image processing. In this paper the hierarchy of the software structure and various application functions are presented. The flexibility of the software to handle different CCDs and also mosaic arrangement is illustrated.

  18. SimITK: visual programming of the ITK image-processing library within Simulink.

    PubMed

    Dickinson, Andrew W L; Abolmaesumi, Purang; Gobbi, David G; Mousavi, Parvin

    2014-04-01

    The Insight Segmentation and Registration Toolkit (ITK) is a software library used for image analysis, visualization, and image-guided surgery applications. ITK is a collection of C++ classes that poses the challenge of a steep learning curve should the user not have appropriate C++ programming experience. To remove the programming complexities and facilitate rapid prototyping, an implementation of ITK within a higher-level visual programming environment is presented: SimITK. ITK functionalities are automatically wrapped into "blocks" within Simulink, the visual programming environment of MATLAB, where these blocks can be connected to form workflows: visual schematics that closely represent the structure of a C++ program. The heavily templated C++ nature of ITK does not facilitate direct interaction between Simulink and ITK; an intermediary is required to convert respective data types and allow intercommunication. As such, a SimITK "Virtual Block" has been developed that serves as a wrapper around an ITK class which is capable of resolving the ITK data types to native Simulink data types. Part of the challenge surrounding this implementation involves automatically capturing and storing the pertinent class information that need to be refined from an initial state prior to being reflected within the final block representation. The primary result from the SimITK wrapping procedure is multiple Simulink block libraries. From these libraries, blocks are selected and interconnected to demonstrate two examples: a 3D segmentation workflow and a 3D multimodal registration workflow. Compared to their pure-code equivalents, the workflows highlight ITK usability through an alternative visual interpretation of the code that abstracts away potentially confusing technicalities. PMID:24402456

  19. Binary Image Classification: A Genetic Programming Approach to the Problem of Limited Training Instances.

    PubMed

    Al-Sahaf, Harith; Zhang, Mengjie; Johnston, Mark

    2016-01-01

    In the computer vision and pattern recognition fields, image classification represents an important yet difficult task. It is a challenge to build effective computer models to replicate the remarkable ability of the human visual system, which relies on only one or a few instances to learn a completely new class or an object of a class. Recently we proposed two genetic programming (GP) methods, one-shot GP and compound-GP, that aim to evolve a program for the task of binary classification in images. The two methods are designed to use only one or a few instances per class to evolve the model. In this study, we investigate these two methods in terms of performance, robustness, and complexity of the evolved programs. We use ten data sets that vary in difficulty to evaluate these two methods. We also compare them with two other GP and six non-GP methods. The results show that one-shot GP and compound-GP outperform or achieve results comparable to competitor methods. Moreover, the features extracted by these two methods improve the performance of other classifiers with handcrafted features and those extracted by a recently developed GP-based method in most cases. PMID:25700148

  20. Algorithms and programming tools for image processing on the MPP, part 2

    NASA Technical Reports Server (NTRS)

    Reeves, Anthony P.

    1986-01-01

    A number of algorithms were developed for image warping and pyramid image filtering. Techniques were investigated for the parallel processing of a large number of independent irregular shaped regions on the MPP. In addition some utilities for dealing with very long vectors and for sorting were developed. Documentation pages for the algorithms which are available for distribution are given. The performance of the MPP for a number of basic data manipulations was determined. From these results it is possible to predict the efficiency of the MPP for a number of algorithms and applications. The Parallel Pascal development system, which is a portable programming environment for the MPP, was improved and better documentation including a tutorial was written. This environment allows programs for the MPP to be developed on any conventional computer system; it consists of a set of system programs and a library of general purpose Parallel Pascal functions. The algorithms were tested on the MPP and a presentation on the development system was made to the MPP users group. The UNIX version of the Parallel Pascal System was distributed to a number of new sites.

  1. In vivo detection and imaging of phosphatidylserine expression during programmed cell death

    PubMed Central

    Blankenberg, Francis G.; Katsikis, Peter D.; Tait, Jonathan F.; Davis, R. Eric; Naumovski, Louis; Ohtsuki, Katsuichi; Kopiwoda, Susan; Abrams, Michael J.; Darkes, Marilyn; Robbins, Robert C.; Maecker, Holden T.; Strauss, H.W.

    1998-01-01

    One of the earliest events in programmed cell death is the externalization of phosphatidylserine, a membrane phospholipid normally restricted to the inner leaflet of the lipid bilayer. Annexin V, an endogenous human protein with a high affinity for membrane bound phosphatidylserine, can be used in vitro to detect apoptosis before other well described morphologic or nuclear changes associated with programmed cell death. We tested the ability of exogenously administered radiolabeled annexin V to concentrate at sites of apoptotic cell death in vivo. After derivatization with hydrazinonicotinamide, annexin V was radiolabeled with technetium 99m. In vivo localization of technetium 99m hydrazinonicotinamide-annexin V was tested in three models: fuminant hepatic apoptosis induced by anti-Fas antibody injection in BALB/c mice; acute rejection in ACI rats with transplanted heterotopic PVG cardiac allografts; and cyclophosphamide treatment of transplanted 38C13 murine B cell lymphomas. External radionuclide imaging showed a two- to sixfold increase in the uptake of radiolabeled annexin V at sites of apoptosis in all three models. Immunohistochemical staining of cardiac allografts for exogenously administered annexin V revealed intense staining of numerous myocytes at the periphery of mononuclear infiltrates of which only a few demonstrated positive apoptotic nuclei by the terminal deoxynucleotidyltransferase-mediated UTP end labeling method. These results suggest that radiolabeled annexin V can be used in vivo as a noninvasive means to detect and serially image tissues and organs undergoing programmed cell death. PMID:9600968

  2. Uncluttered Single-Image Visualization of Vascular Structures using GPU and Integer Programming

    PubMed Central

    Won, Joong-Ho; Jeon, Yongkweon; Rosenberg, Jarrett; Yoon, Sungroh; Rubin, Geoffrey D.; Napel, Sandy

    2013-01-01

    Direct projection of three-dimensional branching structures, such as networks of cables, blood vessels, or neurons onto a 2D image creates the illusion of intersecting structural parts and creates challenges for understanding and communication. We present a method for visualizing such structures, and demonstrate its utility in visualizing the abdominal aorta and its branches, whose tomographic images might be obtained by computed tomography or magnetic resonance angiography, in a single two-dimensional stylistic image, without overlaps among branches. The visualization method, termed uncluttered single-image visualization (USIV), involves optimization of geometry. This paper proposes a novel optimization technique that utilizes an interesting connection of the optimization problem regarding USIV to the protein structure prediction problem. Adopting the integer linear programming-based formulation for the protein structure prediction problem, we tested the proposed technique using 30 visualizations produced from five patient scans with representative anatomical variants in the abdominal aortic vessel tree. The novel technique can exploit commodity-level parallelism, enabling use of general-purpose graphics processing unit (GPGPU) technology that yields a significant speedup. Comparison of the results with the other optimization technique previously reported elsewhere suggests that, in most aspects, the quality of the visualization is comparable to that of the previous one, with a significant gain in the computation time of the algorithm. PMID:22291148

  3. A Large State Medicaid Outpatient Advanced Imaging Utilization Management Program: Substantial Savings Without the Need for Denials.

    PubMed

    Rapoport, Robert J; Parker, Laurence; Levin, David C; Hiatt, Mark D

    2016-06-01

    A decade of rapidly rising outpatient advanced imaging utilization ended toward the end of the past decade, with slow growth since. This has been attributed to repetitive reimbursement cuts, medical radiation exposure concerns, increasing deductibles and patient copayments, and the influence of radiology benefit management companies. State Medicaid programs have been reluctant to institute radiology benefit management preauthorization programs since the time burden for obtaining test approval could cause providers to drop out. Also, these patients may lack the knowledge to appeal denials, and medically necessary tests could be denied with adverse outcomes. Little data exist demonstrating the efficacy of such programs in decreasing utilization and cost. We report a 2-year experience with an outpatient advanced imaging prior notification program for a large state Medicaid fee-for-service population. The program did not allow any denials, but nevertheless the data reveal a large, durable decrease in advanced imaging utilization and cost. PMID:26416792

  4. Novel cis-trans interactions are involved in post-transcriptional regulation of cyclin-dependent kinase inhibitor p21WAF1/CIP1 mRNA

    PubMed Central

    2010-01-01

    Background A variety of pathways target CDKI p21WAF1/CIP1 expression at transcriptional, post-transcriptional as well as translational levels. We previously found that cell growth suppressing retinoid CD437 enhanced expression of p21WAF1/CIP1 and DNA damage inducible GADD45 proteins in part by elevating their mRNA stability. Results Here, we investigated molecular mechanisms of CD437-dependent post-transcriptional regulation of p21WAF1/CIP1 expression. By utilizing MDA-MB-468 HBC cells expressing chimeric rabbit β-globin-p21WAF1/CIP1 transcripts we mapped multiple CD437-responsive sequences located within positions 1195 to 1795 of the 3'-untranslated region of p21WAF1/CIP1 mRNA. Several cytoplasmic proteins present in MDA-MB-468, MCF-7 HBC as well as HL-60R leukemia cells bound specifically, in vitro, with these CD437-responsive sequences. CD437 treatment of cells resulted in elevated binding of ~85 kD and ~55 kD cytoplasmic proteins with putative CD437-responsive sequences. A 12 nt RNA sequence (5'-UGUGGUGGCACA-3') present within CD437-responsive region of p21WAF1/CIP1 mRNA displayed specific and elevated binding with the above noted proteins. Treatment of cells with ActD or CHX prior to CD437 exposure did not abrogate RNA-protein interactions. However, treatment of cytoplasmic protein extracts with proteinase K or alkaline phosphatase resulted in loss of RNA-protein interactions. Conclusions CD437 regulates cell growth in part by regulating stability of p21WAF1/CIP1 mRNA that involves specific RNA-protein interactions that are phosphorylation-dependent, while not requiring nascent transcription or protein synthesis. PMID:20704727

  5. An instrument control and data analysis program for NMR imaging and spectroscopy

    SciTech Connect

    Roos, M.S.; Mushlin, R.A.; Veklerov, E.; Port, J.D.; Ladd, C.; Harrison, C.G.

    1988-01-01

    We describe a software environment created to support real-time instrument control and signal acquisition as well as array-processor based signal and image processing in up to five dimensions. The environment is configured for NMR imaging and in vivo spectroscopy. It is designed to provide flexible tools for implementing novel NMR experiments in the research laboratory. Data acquisition and processing operations are programmed in macros which are loaded in assembled from to minimize instruction overhead. Data arrays are dynamically allocated for efficient use of memory and can be mapped directly into disk files. The command set includes primitives for real-time control of data acquisition, scalar arithmetic, string manipulation, branching, a file system and vector operations carried out by an array processor. 6 figs.

  6. Report on the Imaging Workshop for the Genomes to Life Program

    SciTech Connect

    Colson, Steven; Sudar, Damir

    2002-11-01

    DOE's Genomes to Life program seeks to understand the composition and function of biochemical networks and pathways that carry out the essential processes of living organisms. Such understanding is critical for DOE to more effectively address its missions-clean energy, carbon management, bioremediation, and mitigation of bioterrorism. Imaging of living organisms links the genome to function and recognizes many of the steps along the way to understanding how cell function changes with time and environmental challenges. Innovations in imaging, coupled with computational advances, will accelerate scientific discovery and enable biological solutions to energy challenges. GTL has four main goals: (1) Identify and characterize the molecular machines of life-multiprotein complexes that execute cellular functions and govern cell form. (2) Characterize gene regulatory networks. (3) Characterize the functional repertoire of complex microbial communities in their natural environments at the molecular level. (4) Develop computational methods and capabilities to advance understanding of complex biological systems and predict their behavior.

  7. Fundamental remote sensing science research program. Part 1: Status report of the mathematical pattern recognition and image analysis project

    NASA Technical Reports Server (NTRS)

    Heydorn, R. D.

    1984-01-01

    The Mathematical Pattern Recognition and Image Analysis (MPRIA) Project is concerned with basic research problems related to the study of the Earth from remotely sensed measurement of its surface characteristics. The program goal is to better understand how to analyze the digital image that represents the spatial, spectral, and temporal arrangement of these measurements for purposing of making selected inference about the Earth.

  8. XMapTools a program for X-ray images processing and thermobarometric studies

    NASA Astrophysics Data System (ADS)

    Lanari, Pierre; Burn, Marco; Loury, Chloé; Vidal, Olivier; Engi, Martin

    2014-05-01

    Metamorphic rocks are made up of a mosaic of local thermodynamic equilibria. They frequently involve minerals that grew at different equilibrium conditions of Pressure (P), Temperature (T) and fO2. The identification of relationships between microstructures and metamorphic conditions can be achieved using continuous P-T estimates in two dimensions. The resulting P-T maps can be derived from standardized X-ray images and superimposed to the observed deformation structures and assemblages at thin section scale. XMapTools 1.6.5 (Lanari et al. 2014; find out more at http://www.xmaptools.com), is a set of MATLAB©-based graphical user interface programs to process electron microprobe X-ray images. XMapTools provides an efficient method to standardize X-ray images (raw intensities) into maps of oxide weight percent compositions. This analytical standardization is done using Castaing's approach, employing internal standards. The classification function allows to automatically separate the different minerals phases and other parts of the maps such as fractures or mineral boundaries using a K-means clustering approach. A set of ~50 external functions is providing in the XMapTools package (1) to calculate structural formulae for common minerals from the standardized analyses, and (2) to estimate the P-T conditions of growth or equilibration, with the semi-empirical geothermobarometry functions. For chemically heterogeneous samples, local effective bulk (LEB) can be derived by means of standardized X-ray images. In addition, two graphical user interface modules Chem2D and TriPlot3D can be used to plot the mineral compositions into binary and ternary diagrams. The program XMapTools can easily be coupled with forward (i.e. rock-specific equilibrium phase diagrams) and inverse (i.e. multi-equilibrium) thermodynamics models to estimate the P-T conditions of crystallization at the microscale. This presentation introduces XMapTools and includes typical examples of its functionality

  9. Mississippi Company Using NASA Software Program to Provide Unique Imaging Service: DATASTAR Success Story

    NASA Technical Reports Server (NTRS)

    2001-01-01

    DATASTAR, Inc., of Picayune, Miss., has taken NASA's award-winning Earth Resources Laboratory Applications (ELAS) software program and evolved it to the point that the company is now providing a unique, spatial imagery service over the Internet. ELAS was developed in the early 80's to process satellite and airborne sensor imagery data of the Earth's surface into readable and useable information. While there are several software packages on the market that allow the manipulation of spatial data into useable products, this is usually a laborious task. The new program, called the DATASTAR Image Processing Exploitation, or DIPX, Delivery Service, is a subscription service available over the Internet that takes the work out of the equation and provides normalized geo-spatial data in the form of decision products.

  10. TH-C-18A-06: Combined CT Image Quality and Radiation Dose Monitoring Program Based On Patient Data to Assess Consistency of Clinical Imaging Across Scanner Models

    SciTech Connect

    Christianson, O; Winslow, J; Samei, E

    2014-06-15

    Purpose: One of the principal challenges of clinical imaging is to achieve an ideal balance between image quality and radiation dose across multiple CT models. The number of scanners and protocols at large medical centers necessitates an automated quality assurance program to facilitate this objective. Therefore, the goal of this work was to implement an automated CT image quality and radiation dose monitoring program based on actual patient data and to use this program to assess consistency of protocols across CT scanner models. Methods: Patient CT scans are routed to a HIPPA compliant quality assurance server. CTDI, extracted using optical character recognition, and patient size, measured from the localizers, are used to calculate SSDE. A previously validated noise measurement algorithm determines the noise in uniform areas of the image across the scanned anatomy to generate a global noise level (GNL). Using this program, 2358 abdominopelvic scans acquired on three commercial CT scanners were analyzed. Median SSDE and GNL were compared across scanner models and trends in SSDE and GNL with patient size were used to determine the impact of differing automatic exposure control (AEC) algorithms. Results: There was a significant difference in both SSDE and GNL across scanner models (9–33% and 15–35% for SSDE and GNL, respectively). Adjusting all protocols to achieve the same image noise would reduce patient dose by 27–45% depending on scanner model. Additionally, differences in AEC methodologies across vendors resulted in disparate relationships of SSDE and GNL with patient size. Conclusion: The difference in noise across scanner models indicates that protocols are not optimally matched to achieve consistent image quality. Our results indicated substantial possibility for dose reduction while achieving more consistent image appearance. Finally, the difference in AEC methodologies suggests the need for size-specific CT protocols to minimize variability in image

  11. Cables1 controls p21/Cip1 protein stability by antagonizing proteasome subunit alpha type 3

    PubMed Central

    Shi, Zhi; Li, Zenggang; Li, Zijian; Cheng, Kejun; Du, Yuhong; Fu, Haian; Khuri, Fadlo R.

    2014-01-01

    The cyclin-dependent kinase inhibitor 1A (CDKN1A), p21/Cip1, is a vital cell cycle regulator, dysregulation of which has been associated with a large number of human malignancies. One critical mechanism that controls p21 function is through its degradation, which allows the activation of its associated cell cycle promoting kinases, CDK2 and CDK4. Thus, delineating how p21 is stabilized and degraded will enhance our understanding of cell growth control and offer a basis for potential therapeutic interventions. Here, we report a novel regulatory mechanism that controls the dynamic status of p21 through its interaction with Cdk5 and Abl enzyme substrate 1 (Cables1). Cables1 has a proposed role as a tumor suppressor. We found that upregulation of Cables1 protein was correlated with increased half-life of p21 protein, which was attributed to Cables1/p21 complex formation and supported by their co-localization in the nucleus. Mechanistically, Cables1 interferes with the proteasome (Prosome, Macropain) subunit alpha type 3 (PSMA3) binding to p21 and protects p21 from PSMA3-mediated proteasomal degradation. Moreover, silencing of p21 partially reverses the ability of Cables1 to induce cell death and inhibit cell proliferation. In further support of a potential pathophysiological role of Cables1, the expression level of Cables1 is tightly associated with p21 in both cancer cell lines and human lung cancer patient tumor samples. Together, these results suggest Cables1 as a novel p21 regulator through maintaining p21 stability, and support the model that the tumor suppressive function of Cables1 occurs at least in part through enhancing the tumor suppressive activity of p21. PMID:24975575

  12. Chesapeake Inundation Prediction System (CIPS): A regional prototype for a national problem

    USGS Publications Warehouse

    Stamey, B.; Smith, W.; Carey, K.; Garbin, D.; Klein, F.; Wang, Hongfang; Shen, J.; Gong, W.; Cho, J.; Forrest, D.; Friedrichs, C.; Boicourt, W.; Li, M.; Koterba, M.; King, D.; Titlow, J.; Smith, E.; Siebers, A.; Billet, J.; Lee, J.; Manning, Douglas R.; Szatkowski, G.; Wilson, D.; Ahnert, P.; Ostrowski, J.

    2007-01-01

    Recent Hurricanes Katrina and Isabel, among others, not only demonstrated their immense destructive power, but also revealed the obvious, crucial need for improved storm surge forecasting and information delivery to save lives and property in future storms. Current operational methods and the storm surge and inundation products do not adequately meet requirements needed by Emergency Managers (EMs) at local, state, and federal levels to protect and inform our citizens. The Chesapeake Bay Inundation Prediction System (CIPS) is being developed to improve the accuracy, reliability, and capability of flooding forecasts for tropical cyclones and non-tropical wind systems such as nor'easters by modeling and visualizing expected on-land storm-surge inundation along the Chesapeake Bay and its tributaries. An initial prototype has been developed by a team of government, academic and industry partners through the Chesapeake Bay Observing System (CBOS) of the Mid-Atlantic Coastal Ocean Observing Regional Association (MACOORA) within the Integrated Ocean Observing System (IOOS). For demonstration purposes, this initial prototype was developed for the tidal Potomac River in the Washington, DC metropolitan area. The preliminary information from this prototype shows great potential as a mechanism by which NOAA National Weather Service (NWS) Forecast Offices (WFOs) can provide more specific and timely forecasts of likely inundation in individual localities from significant storm surge events. This prototype system has shown the potential to indicate flooding at the street level, at time intervals of an hour or less, and with vertical resolution of one foot or less. This information will significantly improve the ability of EMs and first responders to mitigate life and property loss and improve evacuation capabilities in individual communities. This paper provides an update and expansion of the initial prototype that was presented at the Oceans 2006 MTS/IEEE Conference in Boston, MA

  13. Arsenic trioxide phosphorylates c-Fos to transactivate p21{sup WAF1/CIP1} expression

    SciTech Connect

    Liu Zimiao; Huang, H.-S.

    2008-12-01

    An infamous poison, arsenic also has been used as a drug for nearly 2400 years; in recently years, arsenic has been effective in the treatment of acute promyelocytic leukemia. Increasing evidence suggests that opposite effects of arsenic trioxide (ATO) on tumors depend on its concentrations. For this reason, the mechanisms of action of the drug should be elucidated, and it should be used therapeutically only with extreme caution. Previously, we demonstrated the opposing effects of ERK1/2 and JNK on p21{sup WAF1/CIP1} (p21) expression in response to ATO in A431 cells. In addition, JNK phosphorylates c-Jun (Ser{sup 63/73}) to recruit TGIF/HDAC1 to suppress p21 gene expression. Presently, we demonstrated that a high concentration of ATO sustains ERK1/2 phosphorylation, and increases c-Fos biosynthesis and stability, which enhances p21 gene expression. Using site-directed mutagenesis, a DNA affinity precipitation assay, and functional assays, we demonstrated that phosphorylation of the C-terminus of c-Fos (Thr{sup 232}, Thr{sup 325}, Thr{sup 331}, and Ser{sup 374}) plays an important role in its binding to the p21 promoter, and in conjunction with N-terminus phosphorylation of c-Fos (Ser{sup 70}) to transactivate p21 promoter expression. In conclusion, a high concentration of ATO can sustain ERK1/2 activation to enhance c-Fos expression, then dimerize with dephosphorylated c-Jun (Ser{sup 63/73}) and recruit p300/CBP to the Sp1 sites (- 84/- 64) to activate p21 gene expression in A431 cells.

  14. Histone deacetylase 3 represses p15{sup INK4b} and p21{sup WAF1/cip1} transcription by interacting with Sp1

    SciTech Connect

    Huang Weifeng; Tan Dapeng; Wang Xiuli; Han Songyan; Tan Jiang; Zhao Yanmei; Lu Jun . E-mail: ycsuo@nenu.edu.cn; Huang Baiqu

    2006-01-06

    Histone deacetylase 3 (HDAC3) has been implicated to play roles in governing cell proliferation. Here we demonstrated that the overexpression of HDAC3 repressed transcription of p15{sup INK4b} and p21{sup WAF1/cip1} genes in 293T cells, and that the recruitment of HDAC3 to the promoter regions of these genes was critical to this repression. We also showed that HDAC3 repressed GAL4-Sp1 transcriptional activity, and that Sp1 was co-immunoprecipitated with FLAG-tagged HDAC3. We conclude that HDAC3 can repress p15{sup INK4b} and p21{sup WAF1/cip1} transcription by interacting with Sp1. Furthermore, knockdown of HDAC3 by RNAi up-regulated the transcriptional expression of p15{sup INK4b}, but not that of p21{sup WAF1/cip1}, implicating the different roles of HDAC3 in repression of p15{sup INK4b} and p21{sup WAF1/cip1} transcription. Data from this study indicate that the inhibition of p15{sup INK4b} and p21{sup WAF1/cip1} may be one of the mechanisms by which HDAC3 participates in cell cycle regulation and oncogenesis.

  15. miR-299-5p promotes cell growth and regulates G1/S transition by targeting p21Cip1/Waf1 in acute promyelocytic leukemia

    PubMed Central

    WU, SHUN-QUAN; ZHANG, LANG-HUI; HUANG, HAO-BO; LI, YA-PING; NIU, WEN-YAN; ZHAN, RONG

    2016-01-01

    MicroRNAs (miRs) are often located in genomic breakpoint regions and are hypothesized to be important regulators involved in the regulation of critical cell processes, including cell apoptosis, proliferation and differentiation. miR-299 has been reported to be upregulated in acute promyelocytic leukemia (APL); however, the function and mechanistic role of miR-299 in APL remains unknown. The present study demonstrated mir-299 significantly induced cell growth and cell cycle progression at the G1/S transition in APL cells. Notably, the present study revealed that miR-299-5p induces these effects, whereas miR-299-3p does not. Additional studies demonstrated that in APL cells the tumor suppressor p21Cip1/Waf1 is a downstream target of miR-299; miR-299 binds directly to the 3′ untranslated region of p21Cip1/Waf1, and reduces protein, but not mRNA, levels of p21Cip1/Waf1. The present findings demonstrate that miR-299 exerts growth-promoting effects in APL cells through the suppression of p21Cip1/Waf1. Overall, the present study demonstrates that p21Cip1/Waf1 is a direct functional target of miR-299 in APL. PMID:27347210

  16. Fundamental remote science research program. Part 2: Status report of the mathematical pattern recognition and image analysis project

    NASA Technical Reports Server (NTRS)

    Heydorn, R. P.

    1984-01-01

    The Mathematical Pattern Recognition and Image Analysis (MPRIA) Project is concerned with basic research problems related to the study of he Earth from remotely sensed measurements of its surface characteristics. The program goal is to better understand how to analyze the digital image that represents the spatial, spectral, and temporal arrangement of these measurements for purposing of making selected inferences about the Earth. This report summarizes the progress that has been made toward this program goal by each of the principal investigators in the MPRIA Program.

  17. A Population-Wide Screening and Tailored Intervention Platform for Eating Disorders on College Campuses: The Healthy Body Image Program

    PubMed Central

    Jones, Megan; Kass, Andrea E.; Trockel, Mickey; Glass, Alan I.; Wilfley, Denise E.; Taylor, C. Barr

    2014-01-01

    Objectives This paper presents a new approach to intervention for eating disorders and body image concerns on college campuses, using a model of integrated eating disorder screening and intervention. Formative data on implementation feasibility are presented. Participants College students enrolled at two universities between 2011–2012. Methods The Healthy Body Image program is an evidence-based screening and intervention platform, enacted via community and online resources. An online screen was used to identify students at varying levels of risk or eating disorder symptom status; responses were used to direct students to universal or targeted online interventions or further evaluation. Universal prevention programs to improve healthy weight regulation and body image culture were offered to all students. Results Formative data from 1,551 students illustrates the application of this model. Conclusions The Healthy Body Image program is feasible to deliver and provides a comprehensive system of screening, evidence-based intervention, and community culture change. PMID:24621000

  18. Parent-Classroom Interaction Program: The Home Needs Assessment.

    ERIC Educational Resources Information Center

    Bevan, Janice

    The Home Needs Assessment is the foundation of the Parent-Classroom Interaction Program (P-CIP) of the Title 6B Demonstration Project for the Profoundly Retarded in Dade County, Florida. The Needs Assessment is usually administered during a home visit by a parent coordinator, is designed to gather information in areas such as family make-up and…

  19. Image Display and Manipulation System (IDAMS) program documentation, Appendixes A-D. [including routines, convolution filtering, image expansion, and fast Fourier transformation

    NASA Technical Reports Server (NTRS)

    Cecil, R. W.; White, R. A.; Szczur, M. R.

    1972-01-01

    The IDAMS Processor is a package of task routines and support software that performs convolution filtering, image expansion, fast Fourier transformation, and other operations on a digital image tape. A unique task control card for that program, together with any necessary parameter cards, selects each processing technique to be applied to the input image. A variable number of tasks can be selected for execution by including the proper task and parameter cards in the input deck. An executive maintains control of the run; it initiates execution of each task in turn and handles any necessary error processing.

  20. Computation of shock waves in media with an interphase boundary by the CIP-CUP method on an adaptive grid

    NASA Astrophysics Data System (ADS)

    Guseva, T. S.

    2016-01-01

    A numerical technique of computing shock waves in compressible media with movable deforming interphase boundaries including those of the gas-liquid type has been realized. The approach without explicit separation of the interphase boundary is applied. The CIP-CUP method is used for integrating the equations of gas dynamics. An adaptive grid of special kind (the soroban-grid) is utilized. Some results of testing the technique using one- and two-dimensional problems are given. Results of computation of impact of a jet on a thin liquid layer on a wall are presented.

  1. The Career Intern Program: Preliminary Results of an Experiment in Career Education. Technical Appendix. Volume 2.

    ERIC Educational Resources Information Center

    Opportunities Industrialization Centers of America, Inc., Philadelphia, PA.

    The technical appendix to "The Career Intern Program: Preliminary Results of an Experiment in Career Education," Volume 1, reports on the research designs used for evaluating the Career Intern Program's (CIP) effectiveness in increasing the student's cognitive skills, academic achievement, vocational adjustment, future orientation, and self-image…

  2. The Career Intern Program: Preliminary Results of an Experiment in Career Education. Volume 1.

    ERIC Educational Resources Information Center

    Gibboney, Richard A.; And Others

    The report describes the first year of development and present operation of the Career Intern Program (CIP), a component of the Urban Career Education Center's alternative school for high school dropouts and potential dropouts. The purpose of the program, operated by the Opportunities Industrialization Centers of America under a contract from the…

  3. Structure of a family 3a carbohydrate-binding module from the cellulosomal scaffoldin CipA of Clostridium thermocellum with flanking linkers: implications for cellulosome structure

    PubMed Central

    Yaniv, Oren; Morag, Ely; Borovok, Ilya; Bayer, Edward A.; Lamed, Raphael; Frolow, Felix; Shimon, Linda J. W.

    2013-01-01

    The cellulosome of the cellulolytic bacterium Clostridium thermocellum has a structural multi-modular protein called CipA (cellulosome-integrating protein A) that includes nine enzyme-binding cohesin modules and a family 3 cellulose-binding module (CBM3a). In the CipA protein, the CBM3a module is located between the second and third cohesin modules and is connected to them via proline/threonine-rich linkers. The structure of CBM3a with portions of the C- and N-terminal flanking linker regions, CBM3a-L, has been determined to a resolution of 1.98 Å. The structure is a β-sandwich with a structural Ca2+ ion. The structure is consistent with the previously determined CipA CBM structure; however, the structured linker regions provide a deeper insight into the overall cellulosome structure and assembly. PMID:23832198

  4. Cloning and DNA Sequencing of the Genes Encoding Clostridium josui Scaffolding Protein CipA and Cellulase CelD and Identification of Their Gene Products as Major Components of the Cellulosome

    PubMed Central

    Kakiuchi, Motohide; Isui, Ayako; Suzuki, Katsuhisa; Fujino, Tsuchiyoshi; Fujino, Emi; Kimura, Tetsuya; Karita, Shuichi; Sakka, Kazuo; Ohmiya, Kunio

    1998-01-01

    The Clostridium josui cipA and celD genes, encoding a scaffolding-like protein (CipA) and a putative cellulase (CelD), respectively, have been cloned and sequenced. CipA, with an estimated molecular weight of 120,227, consists of an N-terminal signal peptide, a cellulose-binding domain of family III, and six successive cohesin domains. The molecular architecture of C. josui CipA is similar to those of the scaffolding proteins reported so far, such as Clostridium thermocellum CipA, Clostridium cellulovorans CbpA, and Clostridium cellulolyticum CipC, but C. josui CipA is considerably smaller than the other scaffolding proteins. CelD consists of an N-terminal signal peptide, a family 48 catalytic domain of glycosyl hydrolase, and a dockerin domain. N-terminal amino acid sequence analysis of the C. josui cellulosomal proteins indicates that both CipA and CelD are major components of the cellulosome. PMID:9696784

  5. CDK inhibitors, p21{sup Cip1} and p27{sup Kip1}, participate in cell cycle exit of mammalian cardiomyocytes

    SciTech Connect

    Tane, Shoji; Ikenishi, Aiko; Okayama, Hitomi; Iwamoto, Noriko; Nakayama, Keiichi I.; Takeuchi, Takashi

    2014-01-17

    Highlights: •Expression of p21 and p27 in the hearts showed a peak during postnatal stages. •p21 and p27 bound to cyclin E, cyclin A and CDK2 in the hearts at postnatal stages. •Cardiomyocytes in both KO mice showed failure in the cell cycle exit at G1-phase. •These data show the first apparent phenotypes in the hearts of Cip/Kip KO mice. -- Abstract: Mammalian cardiomyocytes actively proliferate during embryonic stages, following which cardiomyocytes exit their cell cycle after birth. The irreversible cell cycle exit inhibits cardiac regeneration by the proliferation of pre-existing cardiomyocytes. Exactly how the cell cycle exit occurs remains largely unknown. Previously, we showed that cyclin E- and cyclin A-CDK activities are inhibited before the CDKs levels decrease in postnatal stages. This result suggests that factors such as CDK inhibitors (CKIs) inhibit CDK activities, and contribute to the cell cycle exit. In the present study, we focused on a Cip/Kip family, which can inhibit cyclin E- and cyclin A-CDK activities. Expression of p21{sup Cip1} and p27{sup Kip1} but not p57{sup Kip2} showed a peak around postnatal day 5, when cyclin E- and cyclin A-CDK activities start to decrease. p21{sup Cip1} and p27{sup Kip1} bound to cyclin E, cyclin A and CDK2 at postnatal stages. Cell cycle distribution patterns of postnatal cardiomyocytes in p21{sup Cip1} and p27{sup Kip1} knockout mice showed failure in the cell cycle exit at G1-phase, and endoreplication. These results indicate that p21{sup Cip1} and p27{sup Kip} play important roles in the cell cycle exit of postnatal cardiomyocytes.

  6. Methylosome Protein 50 and PKCδ/p38δ Protein Signaling Control Keratinocyte Proliferation via Opposing Effects on p21Cip1 Gene Expression.

    PubMed

    Saha, Kamalika; Eckert, Richard L

    2015-05-22

    Protein arginine methyltransferase 5 (PRMT5) is a key epigenetic regulator that symmetrically dimethylates arginine residues on histones H3 and H4 to silence gene expression. PRMT5 is frequently observed in a complex with the cofactor methylosome protein 50 (MEP50), which is required for PRMT5 activity. PKCδ/p38δ signaling, a key controller of keratinocyte proliferation and differentiation, increases p21(Cip1) expression to suppress keratinocyte proliferation. We now show that MEP50 enhances keratinocyte proliferation and survival via mechanisms that include silencing of p21(Cip1) expression. This is associated with enhanced PRMT5-MEP50 interaction at the p21(Cip1) promoter and enhanced arginine dimethylation of the promoter-associated histones H3 and H4. It is also associated with a MEP50-dependent reduction in the level of p53, a key controller of p21(Cip1) gene expression. We confirm an important biological role for MEP50 and PRMT5 in regulating keratinocyte proliferation using a stratified epidermal equivalent model that mimics in vivo epidermal keratinocyte differentiation. In this model, PRMT5 or MEP50 knockdown results in reduced keratinocyte proliferation. We further show that PKCδ/p38δ signaling suppresses MEP50 expression, leading to reduced H3/H4 arginine dimethylation at the p21(Cip1) promoter, and that this is associated with enhanced p21(Cip1) expression and reduced cell proliferation. These findings describe an opposing action between PKCδ/p38δ MAPK signaling and PRMT5/MEP50 epigenetic silencing mechanisms in regulating cell proliferation. PMID:25851901

  7. Development of an Automatic Program to Analyze Sunspot Groups on White Light Images using OpenCV

    NASA Astrophysics Data System (ADS)

    Park, J.; Moon, Y.; Choi, S.

    2011-12-01

    Sunspots usually appear in a group which can be classified by certain morphological criteria. In this study we examine the moments which are statistical parameters computed by summing over every pixels of contours, for quantifying the morphological characteristics of a sunspot group. The moments can be another additional characteristics to the sunspot group classification such as McIntosh classification. We are developing a program for image processing, detection of contours and computation of the moments using white light full disk images from Big Bear Solar Observatory. We apply the program to count the sunspot number from 530 white light images in 2003. The sunspot numbers obtained by the program are compared with those by SIDC. The comparison shows that they have a good correlation (r=84%). We are extending this application to automatic sunspot classification (e.g., McIntosh classification) and flare forecasting.

  8. Assessment of the potential suitability of selected commercially available enzymes for cleaning-in-place (CIP) in the dairy industry.

    PubMed

    Boyce, Angela; Piterina, Anna V; Walsh, Gary

    2010-10-01

    The potential suitability of 10 commercial protease and lipase products for cleaning-in-place (CIP) application in the dairy industry was investigated on a laboratory scale. Assessment was based primarily on the ability of the enzymes to remove an experimentally generated milk fouling deposit from stainless steel (SS) panels. Three protease products were identified as being most suitable for this application on the basis of their cleaning performance at 40 °C, which was comparable to that of the commonly used cleaning agent, 1% NaOH at 60 °C. This was judged by quantification of residual organic matter and protein on the SS surface after cleaning and analysis by laser scanning confocal microscopy (LSCM). Enzyme activity was removed/inactivated under conditions simulating those normally undertaken after cleaning (rinsing with water, acid circulation, sanitation). Preliminary process-scale studies strongly suggest that enzyme-based CIP achieves satisfactory cleaning at an industrial scale. Cost analysis indicates that replacing caustic-based cleaning procedures with biodegradable enzymes operating at lower temperatures would be economically viable. Additional potential benefits include decreased energy and water consumption, improved safety, reduced waste generation, greater compatibility with wastewater treatment processes and a reduction in the environmental impact of the cleaning process. PMID:20931416

  9. Segmentation of Whole Cells and Cell Nuclei From 3-D Optical Microscope Images Using Dynamic Programming

    PubMed Central

    McCullough, Dean P.; Gudla, Prabhakar R.; Harris, Bradley S.; Collins, Jason A.; Meaburn, Karen J.; Nakaya, Masa-Aki; Yamaguchi, Terry P.; Misteli, Tom; Lockett, Stephen J.

    2009-01-01

    Communications between cells in large part drive tissue development and function, as well as disease-related processes such as tumorigenesis. Understanding the mechanistic bases of these processes necessitates quantifying specific molecules in adjacent cells or cell nuclei of intact tissue. However, a major restriction on such analyses is the lack of an efficient method that correctly segments each object (cell or nucleus) from 3-D images of an intact tissue specimen. We report a highly reliable and accurate semi-automatic algorithmic method for segmenting fluorescence-labeled cells or nuclei from 3-D tissue images. Segmentation begins with semi-automatic, 2-D object delineation in a user-selected plane, using dynamic programming (DP) to locate the border with an accumulated intensity per unit length greater that any other possible border around the same object. Then the two surfaces of the object in planes above and below the selected plane are found using an algorithm that combines DP and combinatorial searching. Following segmentation, any perceived errors can be interactively corrected. Segmentation accuracy is not significantly affected by intermittent labeling of object surfaces, diffuse surfaces, or spurious signals away from surfaces. The unique strength of the segmentation method was demonstrated on a variety of biological tissue samples where all cells, including irregularly shaped cells, were accurately segmented based on visual inspection. PMID:18450544

  10. What can we expect from near to mid-term direct imaging programs?

    NASA Astrophysics Data System (ADS)

    Boccaletti, A.

    2015-10-01

    Direct imaging for exoplanets has made enormous progress in the last decades owing to the advent of new technologies, efficient algorithms for postprocessing and dedicated observing strategies. A few young giant exoplanets were detected with the previous generation of instruments (beta Pic b, HS3799bcde, HR95086b ...). While SPHERE and GPI were conceived with this very purpose, we are thus expecting many more discoveries in the next years. SPHERE comes with a series of facilities to characterize the atmosphere of these planets, from the visible to the near IR, with broad band, narrow band filters, and low to medium resolution spectroscopy as well. It is also a fabulous instrument to study circumstellar disks both intensity and polarimetry in order to establish the link between planets and their environments. A large survey of 600 targets on a 5 years baseline has been started. The next space telescope, JWST equipped with MIRI and NIRCAM will extend the ability to characterize young giants in the mid IR. NO doubt we will learn more about their atmospheres. Finally, by the next decade, very large apertures will become available on the ground. Extremely Large Telescope will have general first light instruments (MICADO, HARMONI), but some programs to image and characterize young giant planets around very distant stars (>100pc) will be feasible. For much ambitious goal, detecting telluric planets and studying their atmosphere, two paths are now considered either from space (WFIRST AFTA-C is good candidate) and from the Ground with SPHERE-like instruments on ELTs. A review of achievments and perspectives in the context of direct imaging will be presented.

  11. Image data processing system requirements study. Volume 1: Analysis. [for Earth Resources Survey Program

    NASA Technical Reports Server (NTRS)

    Honikman, T.; Mcmahon, E.; Miller, E.; Pietrzak, L.; Yorsz, W.

    1973-01-01

    Digital image processing, image recorders, high-density digital data recorders, and data system element processing for use in an Earth Resources Survey image data processing system are studied. Loading to various ERS systems is also estimated by simulation.

  12. Proceedings of the Second Annual Symposium on Mathematical Pattern Recognition and Image Analysis Program

    NASA Technical Reports Server (NTRS)

    Guseman, L. F., Jr. (Principal Investigator)

    1984-01-01

    Several papers addressing image analysis and pattern recognition techniques for satellite imagery are presented. Texture classification, image rectification and registration, spatial parameter estimation, and surface fitting are discussed.

  13. ENVIRONMENTAL TECHNOLOGY VERIFICATION: Reduction of Nitrogen in Domestic Wastewater from Individual Residential Homes. BioConcepts, Inc. ReCip® RTS ~ 500 System

    EPA Science Inventory

    Verification testing of the ReCip® RTS-500 System was conducted over a 12-month period at the Massachusetts Alternative Septic System Test Center (MASSTC) located on Otis Air National Guard Base in Bourne, Massachusetts. A nine-week startup period preceded the verification test t...

  14. The Group B Streptococcus–Secreted Protein CIP Interacts with C4, Preventing C3b Deposition via the Lectin and Classical Complement Pathways

    PubMed Central

    Pietrocola, Giampiero; Rindi, Simonetta; Rosini, Roberto; Buccato, Scilla

    2016-01-01

    The group B Streptococcus (GBS) is a leading cause of neonatal invasive disease. GBS bacteria are surrounded by a thick capsular polysaccharide that is a potent inhibitor of complement deposition via the alternative pathway. Several of its surface molecules can however activate the classical and lectin complement pathways, rendering this species still vulnerable to phagocytic killing. In this study we have identified a novel secreted protein named complement interfering protein (CIP) that downregulates complement activation via the classical and lectin pathways, but not the alternative pathway. The CIP protein showed high affinity toward C4b and inhibited its interaction with C2, presumably preventing the formation of the C4bC2a convertase. Addition of recombinant CIP to GBS cip-negative bacteria resulted in decreased deposition of C3b on their surface and in diminished phagocytic killing in a whole-blood assay. Our data reveal a novel strategy exploited by GBS to counteract innate immunity and could be valuable for the development of anti-infective agents against this important pathogen. PMID:26608922

  15. The Group B Streptococcus-Secreted Protein CIP Interacts with C4, Preventing C3b Deposition via the Lectin and Classical Complement Pathways.

    PubMed

    Pietrocola, Giampiero; Rindi, Simonetta; Rosini, Roberto; Buccato, Scilla; Speziale, Pietro; Margarit, Immaculada

    2016-01-01

    The group B Streptococcus (GBS) is a leading cause of neonatal invasive disease. GBS bacteria are surrounded by a thick capsular polysaccharide that is a potent inhibitor of complement deposition via the alternative pathway. Several of its surface molecules can however activate the classical and lectin complement pathways, rendering this species still vulnerable to phagocytic killing. In this study we have identified a novel secreted protein named complement interfering protein (CIP) that downregulates complement activation via the classical and lectin pathways, but not the alternative pathway. The CIP protein showed high affinity toward C4b and inhibited its interaction with C2, presumably preventing the formation of the C4bC2a convertase. Addition of recombinant CIP to GBS cip-negative bacteria resulted in decreased deposition of C3b on their surface and in diminished phagocytic killing in a whole-blood assay. Our data reveal a novel strategy exploited by GBS to counteract innate immunity and could be valuable for the development of anti-infective agents against this important pathogen. PMID:26608922

  16. Tsunami Simulation using CIP Method with Characteristic Curve Equations and TVD-MacCormack Method

    NASA Astrophysics Data System (ADS)

    Fukazawa, Souki; Tosaka, Hiroyuki

    2015-04-01

    After entering 21st century, we already had two big tsunami disasters associated with Mw9 earthquakes in Sumatra and Japan. To mitigate the damages of tsunami, the numerical simulation technology combined with information technologies could provide reliable predictions in planning countermeasures to prevent the damage to the social system, making safety maps, and submitting early evacuation information to the residents. Shallow water equations are still solved not only for global scale simulation of the ocean tsunami propagation but also for local scale simulation of overland inundation in many tsunami simulators though three-dimensional model starts to be used due to improvement of CPU. One-dimensional shallow water equations are below: partial bm{Q}/partial t+partial bm{E}/partial x=bm{S} in which bm{Q}=( D M )), bm{E}=( M M^2/D+gD^2/2 )), bm{S}=( 0 -gDpartial z/partial x-gn2 M|M| /D7/3 )). where D[m] is total water depth; M[m^2/s] is water flux; z[m] is topography; g[m/s^2] is the gravitational acceleration; n[s/m1/3] is Manning's roughness coefficient. To solve these, the staggered leapfrog scheme is used in a lot of wide-scale tsunami simulator. But this scheme has a problem that lagging phase error occurs when courant number is small. In some practical simulation, a kind of diffusion term is added. In this study, we developed two wide-scale tsunami simulators with different schemes and compared usual scheme and other schemes in practicability and validity. One is a total variation diminishing modification of the MacCormack method (TVD-MacCormack method) which is famous for the simulation of compressible fluids. The other is the Cubic Interpolated Profile (CIP) method with characteristic curve equations transformed from shallow water equations. Characteristic curve equations derived from shallow water equations are below: partial R_x±/partial t+C_x±partial R_x±/partial x=∓ g/2partial z/partial x in which R_x±=√{gD}± u/2, C_x±=u± √{gD}. where u

  17. Between Education and the World of Work; The Image of the World of Work. Occupational Education Program.

    ERIC Educational Resources Information Center

    Bush, Donald O.; And Others

    The activity Image of the World of Work, part of the Occupational Education Program of the Rocky Mountain Educational Laboratory, RMEL, is designed to influence and nurture positive work relevant attitudes of seventh grade pupils as well as to increase pupils' knowledge of occupational information and career alternatives. The workshops designed to…

  18. Advanced electro-optical imaging techniques. [conference papers on sensor technology applicable to Large Space Telescope program

    NASA Technical Reports Server (NTRS)

    Sobieski, S. (Editor); Wampler, E. J. (Editor)

    1973-01-01

    The papers presented at the symposium are given which deal with the present state of sensors, as may be applicable to the Large Space Telescope (LST) program. Several aspects of sensors are covered including a discussion of the properties of photocathodes and the operational imaging camera tubes.

  19. Covenant model of corporate compliance. "Corporate integrity" program meets mission, not just legal, requirements.

    PubMed

    Tuohey, J F

    1998-01-01

    Catholic healthcare should establish comprehensive compliance strategies, beyond following Medicare reimbursement laws, that reflect mission and ethics. A covenant model of business ethics--rather than a self-interest emphasis on contracts--can help organizations develop a creed to focus on obligations and trust in their relationships. The corporate integrity program (CIP) of Mercy Health System Oklahoma promotes its mission and interests, educates and motivates its employees, provides assurance of systemwide commitment, and enforces CIP policies and procedures. Mercy's creed, based on its mission statement and core values, articulates responsibilities regarding patients and providers, business partners, society and the environment, and internal relationships. The CIP is carried out through an integrated network of committees, advocacy teams, and an expanded institutional review board. Two documents set standards for how Mercy conducts external affairs and clarify employee codes of conduct. PMID:10181597

  20. Video Extrapolation Method Based on Time-Varying Energy Optimization and CIP.

    PubMed

    Sakaino, Hidetomo

    2016-09-01

    Video extrapolation/prediction methods are often used to synthesize new videos from images. For fluid-like images and dynamic textures as well as moving rigid objects, most state-of-the-art video extrapolation methods use non-physics-based models that learn orthogonal bases from a number of images but at high computation cost. Unfortunately, data truncation can cause image degradation, i.e., blur, artifact, and insufficient motion changes. To extrapolate videos that more strictly follow physical rules, this paper proposes a physics-based method that needs only a few images and is truncation-free. We utilize physics-based equations with image intensity and velocity: optical flow, Navier-Stokes, continuity, and advection equations. These allow us to use partial difference equations to deal with the local image feature changes. Image degradation during extrapolation is minimized by updating model parameters, where a novel time-varying energy balancer model that uses energy based image features, i.e., texture, velocity, and edge. Moreover, the advection equation is discretized by high-order constrained interpolation profile for lower quantization error than can be achieved by the previous finite difference method in long-term videos. Experiments show that the proposed energy based video extrapolation method outperforms the state-of-the-art video extrapolation methods in terms of image quality and computation cost. PMID:27305677

  1. The Effect of “In Favor of Myself”: Preventive Program to Enhance Positive Self and Body Image among Adolescents

    PubMed Central

    Golan, Moria; Hagay, Noaa; Tamir, Snait

    2013-01-01

    Background Positive self-esteem, emotional well-being, school achievements and family connectedness are considered protective factors against health-compromising behaviors. This study examined the effect of an interactive, community-based, media literacy and dissonance wellness program, In Favor of Myself, on the self-image, body image, eating attitudes and behavior of young adolescents. A preliminary cohort study was conducted among 972 program participants who did not take part in the controlled trial. Over 75% of participants said they would recommend the program to their friends. Methods A controlled trial was conducted to evaluate program acceptability, efficacy and effectiveness among 259 participants (210 in the intervention group and 49 in the control group), aged 12–14 years, who completed questionnaires during at least two assessment times. Program materials were provided, along with leaders' training, in order to ensure quality program delivery and creation of a wide network of committed program leaders. Results The program significantly reduced drive for thinness and self-worth contingent upon others' approval, the gap between current body figure and ideal figure, and the impact of mood on girls' self-image. Superiority was found among those participating in the intervention group with respect to recognizing media strategies, the influence of media on desire to change, and the influence of appearance on self-confidence and drive for thinness. Conclusions In Favor of Myself shows promising results for strengthening adolescents' ability to cope with the challenges of their life stage. Suggestions for improving In Favor of Myself are presented. PMID:24265681

  2. Protective effects of calcitriol on diabetic nephropathy are mediated by down regulation of TGF-β1 and CIP4 in diabetic nephropathy rat

    PubMed Central

    Yu, Rui; Mao, Jiangfeng; Yang, Ye; Zhang, Yuanyuan; Tian, Yanyan; Zhu, Jun

    2015-01-01

    Objective: To explore the protective effects of calcitriol on diabetic nephropathy by modulating the expressions of transforming growth factor-beta 1 (TGF-β1) and Cdc42 interacting protein-4 (CIP4). Methods: Streptozotocin-induced diabetic nephropathy rats (n=36) were randomly divided into control group (control-H, control-M, control-L) and calcitriol group (calcitriol-H, calcitriol-M, calcitriol-L). The expression of TGF-β1 gradually decreased in control-H, control-M and control-L subgroups by injection of different virus vectors. Peanut oil and calcitriol were given to control and calcitriol group, respectively. The expressions of TGF-β1 and CIP4 in kidney, the pathology, and the renal function and lipid profiles were compared between control and calcitriol treatment groups. Results: In the control group, the higher level of TGF-β1 was associated with more severe glomerular pathology (P<0.05). There is a positive correlation between the expression of CIP4 and TGF-β1. Control-H subgroup had significant more severe kidney disease, higher levels of cholesterol, triglyceride, blood glucose, blood urea nitrogen (BUN) and creatinine (Cr) than control-M and control-L subgroups. After calcitriol treatment, the expression of TGF-β1 and CIP4 were significantly decreased compared to the corresponding control subgroups (all P<0.05). Renal fibrosis and pathological changes were markedly improved. The levels of cholesterol, triglyceride, blood glucose, BUN and Cr were significantly reduced (P<0.05). Conclusion: Calcitriol may protect diabetic nephropathy from fibrosis via inhibition of TGF-β1 and CIP4. PMID:26097534

  3. Low inducible expression of p21Cip1 confers resistance to paclitaxel in BRAF mutant melanoma cells with acquired resistance to BRAF inhibitor.

    PubMed

    Jang, Gun-Hee; Kim, Na-Yeon; Lee, Michael

    2015-08-01

    The therapeutic efficacy of oncogenic BRAF inhibitor is limited by the onset of acquired resistance. In this study, we investigated the potential therapeutic effects of the mitotic inhibitor paclitaxel on three melanoma cell lines with differing sensitivity to the BRAF inhibitor. Of the two BRAF inhibitor-resistant cell lines, A375P/Mdr cells harboring the BRAF V600E mutant were resistant and the wild-type BRAF SK-MEL-2 cells were sensitive to paclitaxel. In particular, paclitaxel caused the growth inhibition of SK-MEL-2 cells to a much greater extent than it caused growth inhibition of A375P cells. Paclitaxel exhibited no significant effect on the phosphorylation of MEK-ERK in any cell lines tested, regardless of both the BRAF mutation and the drug resistance, implying that paclitaxel activity is independent of MEK-ERK inhibition. In A375P cells, paclitaxel treatment resulted in a marked emergence of apoptotic cells after mitotic arrest, concomitant with a remarkable induction of p21(Cip1). However, paclitaxel only moderately increased the levels of p21(Cip1) in A375P/Mdr cells, which exhibited a strong resistance to paclitaxel. The p21(Cip1) overexpression partially conferred paclitaxel sensitivity to A375P/Mdr cells. Interestingly, we found an extremely low background expression level of p21(Cip1) in SK-MEL-2 cells lacking normal p53 function, which caused much greater G2/M arrest than that seen in A375P cells. Taken together, these results suggest that paclitaxel may be an effective anticancer agent through regulating the expression of p21(Cip1) for the treatment of BRAF mutant melanoma cells resistant to BRAF inhibitors. PMID:25912549

  4. Development of Educational Support System for Learning Image Processing Enabling Client-Side Programming Aided by Java Servlet Technology

    NASA Astrophysics Data System (ADS)

    Furukawa, Tatsuya; Aoki, Noriyuki; Ohchi, Masashi; Nakao, Masaki

    The image proccessing has become a useful and important technology in various reserch and development fields. According to such demands for engineering problems, we have designed and implemented the educational support system for that using a Java Applet technology. However in the conventional system, it required the tedious procedure for the end user to code his own programs. Therefore, in this study, we have improved the defect in the previous system by using a Java Servlet technology. The new system will make it possible for novice user to experience a practical digital image proccessing and an advanced programming with ease. We will describe the architecture of the proposed system function, that has been introduced to facilitate the client-side programming.

  5. Image transformation into device-dependent color printer description using 4th-order polynomial regression and object-oriented programming development of image processing modules

    NASA Astrophysics Data System (ADS)

    Mongeon, Michael C.

    1996-03-01

    This paper investigates the development of printer device profiles used in color document printing system environments when devices with intrinsically different gamut capabilities communicate with one another in a common (CIELAB) color space. While the main thrust of this activity focuses on the output printer, namely the Xerox 5760 printer, and its rendition of some device independent image description, characterizations are provided which investigate relative areas of photographic, monitor, and printer gamuts using a visual hue leaf comparison between devices. The printer is modeled using 4th-order polynomial regression which maps the device independent CIELAB image representation into device dependent printer CMYK. This technique results in 1.89 AEEavg over the training data set. Some key properties of the proposed calibration method are as follows: (1) Linearized CMYK tone reproduction curves with respect to AEEpaper to improve the distribution of calibration data in color space. (2) Application of GCR strategy and linearization to the calibration target prior to the regression on the measured CIELAB and original CMY values. Each strategy employs a K addition/No CMY removal method which maximizes printer gamut and relies on the regression to determine the appropriate CMY removal. The following GCR strategies are explored: CMY only (0% K addition), 50% K addition, 100% K addition, and non-linear K addition. A library of image processing algorithms is included, using LabView object oriented programming, which provides a modular approach for key color processing tasks. In the user interface, an image is selected with appropriate GCR strategy, and the program operates on the image. In general, the pictorial image quality is excellent for each GCR strategy with subtle differences between GCR approaches. Quantitative analysis of Q60 color matching performance is included.

  6. xdamp Version 3: An IDL{reg_sign}-based data and image manipulation program

    SciTech Connect

    Ballard, W.P.

    1998-05-01

    The original DAMP (DAta Manipulation Program) was written by Mark Hedemann of Sandia National Laboratories and used the CA-DISSPLA{trademark} (available from Computer Associates International, Inc., Garden City, NY) graphics package as its engine. It was used to plot, modify, and otherwise manipulate the one-dimensional data waveforms (data vs. time) from a wide variety of accelerators. With the waning of CA-DISSPLA and the increasing popularity of Unix{reg_sign}-based workstations, a replacement was needed. This package uses the IDL{reg_sign} software, available from Research Systems Incorporated in Boulder, Colorado, as the engine, and creates a set of widgets to manipulate the data in a manner similar to the original DAMP and earlier versions of xdamp. IDL is currently supported on a wide variety of Unix platforms such as IBM{reg_sign} workstations, Hewlett Packard workstations, SUN{reg_sign} workstations, Microsoft{reg_sign} Windows{trademark} computers, Macintosh{reg_sign} computers and Digital Equipment Corporation VMS{reg_sign} and Alpha{reg_sign} systems. Thus, xdamp is portable across many platforms. The author has verified operation, albeit with some minor IDL bugs, on personal computers using Windows 95 and Windows NT; IBM Unix platforms; and DEC alpha and VMS systems; HP 9000/700 series workstations; and Macintosh computers, both regular and PowerPC{trademark} versions. Version 3 adds the capability to manipulate images to the original xdamp capabilities.

  7. Removing a Nail from the Boot Camp Coffin: An Outcome Evaluation of Minnesota's Challenge Incarceration Program

    ERIC Educational Resources Information Center

    Duwe, Grant; Kerschner, Deborah

    2008-01-01

    Using a retrospective, quasiexperimental design, this study evaluates Minnesota's Challenge Incarceration Program (CIP), examining whether it has lowered recidivism and saved money. In addition to utilizing a lengthy follow-up period and multiple measures of recidivism and participation, a multistage sampling design was employed to create a…

  8. Superintendent's Recommended FY 2011 Capital Budget and the FY 2011-2016 Capital Improvements Program

    ERIC Educational Resources Information Center

    Montgomery County Public Schools, 2009

    2009-01-01

    In November 1996, the voters of Montgomery County approved by referendum an amendment to the County Charter that changed the County Council's review and approval cycle of the six-year Capital Improvements Program (CIP) from an annual to biennial cycle. The referendum specified that in odd-numbered fiscal years (on-years) the County Council would…

  9. 75 FR 43178 - Medicare Program; Solicitation for Proposals for the Medicare Imaging Demonstration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-23

    ... defined in Sec. 1834(e)(1)(B) of the Social Security Act (the Act): Diagnostic magnetic resonance imaging... the Medicare Imaging Demonstration AGENCY: Centers for Medicare & Medicaid Services (CMS), HHS. ACTION... an opportunity to apply to participate in the Medicare Imaging Demonstration (MID) that...

  10. Programs.

    ERIC Educational Resources Information Center

    Community College Journal, 1996

    1996-01-01

    Includes a collection of eight short articles describing model community college programs. Discusses a literacy program, a mobile computer classroom, a support program for at-risk students, a timber-harvesting program, a multimedia presentation on successful women graduates, a career center, a collaboration with NASA, and an Israeli engineering…

  11. An outdoor adventure program for young adults with cancer: positive effects on body image and psychosocial functioning.

    PubMed

    Rosenberg, Robin S; Lange, Whitney; Zebrack, Brad; Moulton, Samuel; Kosslyn, Stephen M

    2014-01-01

    This study evaluated the psychological effects of an outdoor adventure program on young adult cancer survivors (ages 18-39). The 6-day adventure program included personal instruction and supervision on the basics of kayaking, surfing, or climbing. Compared to a wait-list control group, participants who took part in the program for the first time had improved (relative to pretest) body image, self-compassion and self-esteem, and less depression and alienation. Participants who took part for the second time, though also helped by the program in similar ways, were no better off psychologically than participants who took part for the first time. Possible explanations for the positive effects and their apparent short duration are offered. PMID:24988227

  12. 2010 Mississippi Curriculum Framework: Postsecondary Meat Merchandising Technology. (Program CIP: 12.0506 - Meat Cutting/Meat Cutter)

    ERIC Educational Resources Information Center

    Jones, Roger; Currie, Lamar; Clayton, Sheila

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  13. 2007 Mississippi Curriculum Framework: Postsecondary Meat Merchandising Technology (Program CIP: 12.0506 - Meat Cutting/Meat Cutter)

    ERIC Educational Resources Information Center

    Jones, Roger; Currie, Lamar; Clayton, Sheila

    2007-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  14. 2009 Mississippi Curriculum Framework: Postsecondary Court Reporting Technology. (Program CIP: 22.0303 - Court Reporting/Court Reporter)

    ERIC Educational Resources Information Center

    Adams, Geanell

    2009-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  15. 2007 Mississippi Curriculum Framework: Postsecondary Optometric Assisting Technology. (Program CIP: 51.1802 - Optometric Technician/Assistant)

    ERIC Educational Resources Information Center

    Scott, Veronica

    2007-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  16. 2009 Mississippi Curriculum Framework: Postsecondary Health Information Technology. (Program CIP-51.0707-Medical Records Technology/Technician)

    ERIC Educational Resources Information Center

    Hoffman, Casey; Jones, Robin; McGuffee, Michelle; Scott, Nena

    2009-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  17. 2008 Mississippi Curriculum Framework: Postsecondary Food Production and Management Technology. (Program CIP: 12.0508 - Institutional Food Workers)

    ERIC Educational Resources Information Center

    Carver, Matthew; Hoff, Jody; Little, Lisa; Samuel, Carolyn

    2008-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  18. 2009 Mississippi Curriculum Framework: Postsecondary Funeral Service Technology. (Program CIP-12.0301 - Funeral Service and Mortuary Science)

    ERIC Educational Resources Information Center

    Anderson, Larry; Dickerson, Octavia; Harvey, Bill; Moore, Tony

    2009-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  19. 2010 Mississippi Curriculum Framework: Postsecondary Banking and Finance Technology. (Program CIP: 52.0803 - Banking and Financial Support Services)

    ERIC Educational Resources Information Center

    Gullett, Janet; Dinkins, Shivochie

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  20. Efficacy of the Social Skills Improvement System--Classwide Intervention Program (SSIS-CIP) in the Primary Grades

    ERIC Educational Resources Information Center

    DiPerna, James C.; Lei, Puiwa; Bellinger, Jill; Cheng, Weiyi

    2014-01-01

    Teaching children to get along with others, care about themselves, and actively participate in learning are three of the most important outcomes of the schooling process. Yet children in some schools are not achieving these outcomes, and many educators have not received adequate training to create instructional environments that facilitate these…

  1. 2008 Mississippi Curriculum Framework: Postsecondary Practical Nursing. (Program CIP: 51.3901 - Licensed Practical/Vocational Nurse Training)

    ERIC Educational Resources Information Center

    McNutt, Jana; Clowers, Pat; Collum, Lara; Canton, Dixie; Comfort, Sherri; Kron, Maxine; Mahaffey, Elizabeth; Hancock, Jane; Waldrup, Sandra; Walker, Sherri; Pearson, Lisa; Miller, Kelly; Cooper, Patti; Bedwell, Susan

    2008-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  2. 2011 Mississippi Curriculum Framework: Postsecondary Dental Assisting Technology. (Program CIP: 51.0601 - Dental Assisting/Assistant)

    ERIC Educational Resources Information Center

    Addison, Emily; Bokros, Christy; Gavant, H. Richard; Johnson, Robin

    2011-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  3. 2006 Mississippi Curriculum Framework: Postsecondary Dental Assisting Technology. (Program CIP: 51.0601 - Dental Assisting/Assistant)

    ERIC Educational Resources Information Center

    Addison, Emily; Gavant, H. Richard

    2006-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  4. 2005 Mississippi Curriculum Framework: Postsecondary Banking and Finance Technology. (Program CIP: 52.0803 - Banking and Financial Support Services)

    ERIC Educational Resources Information Center

    Gullett, Janet; Thigpen, Herbert

    2005-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  5. 2008 Mississippi Curriculum Framework: Postsecondary Massage Therapy. (Program CIP: 51.3501 - Massage Therapy/Therapeutic Massage)

    ERIC Educational Resources Information Center

    Patterson, Teresa; Russell, Cydnee

    2008-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  6. 2008 Mississippi Curriculum Framework: Postsecondary Radiologic Technology. (Program CIP: 51.0911 - Radiologic Technology/Science - Radiographer)

    ERIC Educational Resources Information Center

    Armstrong, David; Cochran, Timothy; Compton, Steve; Davis, Jennifer; Edgerton, Seena Shazowee; Kisner, Christie; Lewis, Judy; Sartin, Billie Faye; Shell, Deborah

    2008-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  7. 2008 Mississippi Curriculum Framework: Postsecondary Irrigation Management Technology. (Program CIP:01.0699 - Applied Horticulture/Horticultural Business Services, Other)

    ERIC Educational Resources Information Center

    Oliver, Michael L.

    2008-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  8. 2010 Mississippi Curriculum Framework: Postsecondary Emergency Medical Technician. (Program CIP: 51.0904 - Emergency Medical Technology/Technician)

    ERIC Educational Resources Information Center

    Briscoe, Lisa; Bryant, Katrina; Galtelli, Mark; Glasson, Kristi; Hall, David; Hood, Brenda; Mahaffey, Libby; McBryde, John; Read, John; Shirley, Gary; Wright, Al

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  9. 2009 Mississippi Curriculum Framework: Postsecondary Nuclear Medicine Technology. (Program CIP: 51.0905 - Nuclear Medical Technology/Technologist)

    ERIC Educational Resources Information Center

    Boney, Linda; Lee, Joanne; Pyles, Alice; Whitfield, Stacy

    2009-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  10. 2007 Mississippi Curriculum Framework: Postsecondary Small Engine and Turf Equipment Repair Technology. (Program CIP: 01.0299 Agricultural Mechanization, Other )

    ERIC Educational Resources Information Center

    Spence, Allen; Campus, Raymond

    2007-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  11. 2011 Mississippi Curriculum Framework: Postsecondary Agricultural Technician Technology. (Program CIP: 01.0201 - Agricultural Mechanics and Equipment/Machine Technology)

    ERIC Educational Resources Information Center

    Massey, Jeremy; Louwerens, Shane; Galey, Joe

    2011-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  12. 2007 Mississippi Curriculum Framework: Postsecondary Agricultural Mechanics Technology. (Program CIP: 01.0201 - Agricultural Mechanics and Equipment/Machine Technology)

    ERIC Educational Resources Information Center

    Massey, Jeremy; Louwerens, Shane; Galey, Joe

    2007-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  13. 2011 Mississippi Curriculum Framework: Postsecondary Conservation Law Enforcement Technology. (Program CIP: 03.0208 - Natural Resources Management and Policy, Other)

    ERIC Educational Resources Information Center

    Jones, Luke; Myrick, Dwight

    2011-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  14. Application of graphically oriented programming to imaging of structure deterioration of historic glass by optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Sylwestrzak, Marcin; Kwiatkowska, Ewa A.; Karaszkiewicz, Pawel; Iwanicka, Magdalena; Targowski, Piotr

    2009-07-01

    Optical Coherence Tomography (OCT) is an interferometric method utilising light of low temporal coherence for noninvasive structural imaging of objects weakly absorbing and scattering light. In this contribution, using various examples of images of objects made of glass affected by the atmospheric corrosion and/or by crizzling, we demonstrate a software developed in our laboratory specifically for 3D OCT imaging of samples with a fine structure. For this task we employed the OpenGL platform (Open Graphics Library), an Application Programming Interface (API) for writing applications dedicated to interactive 3D computer graphics. In our application we have utilized texture rendering with a modulation of transparency and a colour as a function of elevation.

  15. 27-day solar forcing of mesospheric temperature, water vapor and polar mesospheric clouds from the AIM SOFIE and CIPS satellite experiments

    NASA Astrophysics Data System (ADS)

    Thomas, Gary; Thurairajah, Brentha; von Savigny, Christian; Hervig, Mark; Snow, Martin

    2016-04-01

    Solar cycle variations of ultraviolet radiation have been implicated in the 11-year and 27-day variations of Polar Mesospheric Cloud (PMC) properties. Both of these variations have been attributed to variable solar ultraviolet heating and photolysis, but no definitive studies of the mechanisms are available. The solar forcing issue is critical toward answering the broader question of whether PMC's have undergone long-term changes, and if so, what is the nature of the responsible long-term climate forcings? One of the principal goals of the Aeronomy of Ice in the Mesosphere satellite mission was to answer the question: "How does changing solar irradiance affect PMCs and the environment in which they form?" We describe an eight-year data set from the AIM Solar Occultation for Ice Experiment (SOFIE) and the AIM Cloud Imaging and Particle Size (CIPS) experiment. Together, these instruments provide high-precision measurements of high-latitude summertime temperature (T), water vapor (H2O), and PMC ice properties for the period 2007-present. The complete temporal coverage of the summertime polar cap region for both the primary atmospheric forcings of PMC (T and H2O), together with a continually updated time series of Lyman-alpha solar irradiance, allows an in-depth study of the causes and effects of 27-day PMC variability. The small responses of these variables, relative to larger day-to-day changes from gravity waves, tides, inter-hemispheric coupling, etc. require a careful statistical analysis to isolate the solar influence. We present results for the 27-day responses of T, H2O and PMC for a total of 15 PMC seasons, (30 days before summer solstice to 60 days afterward, for both hemispheres). We find that the amplitudes and phase relationships are not consistent with the expected mechanisms of solar UV heating and photolysis - instead we postulate a primarily dynamical response, in which a periodic vertical wind heats/cools the upper mesosphere, and modulates PMC

  16. Analysis of p21Waf1/Cip1 expression in normal, premalignant, and malignant cells during the development of human lung adenocarcinoma.

    PubMed Central

    Hayashi, H.; Miyamoto, H.; Ito, T.; Kameda, Y.; Nakamura, N.; Kubota, Y.; Kitamura, H.

    1997-01-01

    Our studies suggested that adenocarcinoma of the peripheral lung mostly develops by several steps from atypical adenomatous hyperplasia through early adenocarcinoma to overt adenocarcinoma, and that some p53 abnormalities play an important role in this progression. In the present study, we examined by immunohistochemistry the expression of p53-inducible cyclin-dependent kinase inhibitor p21Waf1/Cip1 (p21) in the cells at various developmental stages of lung adenocarcinoma (32 lesions of adenomatous hyperplasia, 14 of early adenocarcinoma, 23 of well differentiated adenocarcinoma, and 17 of moderately or poorly differentiated adenocarcinoma) in comparison with 19 reactive proliferative lesions and analyzed the relationship between p53 and p21 expression. Bronchioalveolar cells in the normal lung expressed very little or no p21 and no p53 expression. In not only reactive but also neoplastic lesions regardless of their developmental stage, the cells expressed p21 at various frequencies. The average labeling indices ranged from 5.4 to 13.8%, and there was no significant difference between any of these categories. The expression of p21, however, tended to be relatively low in moderately and poorly differentiated adenocarcinomas (5.5%) compared to well differentiated adenocarcinomas (12.2%), and high-level p21 expressors (10% < or = positive cells) were more frequent in the latter group (1 of 17 (6%) versus 3 of 23 (35%), P < 0.05), suggesting that p21 expression is affected by the degree of differentiation of the neoplastic cells. Although the correlation was positive between the expression of p21 and p53 in reactive lesions (r = 0.88; P < 0.001), none was found in neoplastic lesions at any step or grade (-0.12 < or = r < or = 0.26). These results indicated that p21 expression depends upon p53 expression in reactive lung cells, whereas p21 expression is at least in part independent of that of p53 from the earliest to the most fully developed step of lung adenocarcinoma

  17. Evaluation of An Upper Elementary School Program To Prevent Body Image, Eating, and Weight Concerns.

    ERIC Educational Resources Information Center

    Kater, Kathy J.; Rohwer, John; Londre, Karen

    2002-01-01

    Examined whether the 11-lesson "Healthy Body Image: Teaching Kids to Eat and Love Their Bodies Too!" curriculum would have a positive effect on upper elementary students. Pretest- posttest measures indicated that, compared to control children, children who completed the curriculum showed significant or notable improvement regarding body image,…

  18. Individual variation in p53 and Cip1 expression profiles in normal human fibroblast strains following exposure to high-let radiation

    SciTech Connect

    Carpenter, T.R.; Johnson, N.F.; Gilliland, F.D.

    1995-12-01

    Exposure to {alpha}-particles emitted by radon progeny appears to be the second-leading cause of lung cancer mortality. However, individual susceptibility to the carcinogenic effects of {alpha}-particles remains poorly characterized. Variation in susceptibility to cancer produced by certian classes of DNA-damaging chemicals is suspected to involve differences in metabolic activation and detoxication. Susceptibility to {alpha}-particle-induced cancer may involve variations in capacity or opportunity to repair DNA damage. Subtle variations in DNA repair capacity would more likely explain radon-related lung cancer susceptibility. The p53 tumor suppressor protein accumulates as a cellular response to DNA damage from ionizing radiation and regulates arrest in the G{sub 1} portion of the cell cycle. Arrest in G{sub 1} portion of the cell cycle. While upstream regulation of p53 protein stability is poorly understood, variations in the ability to accumulate p53 following DNA damage represent potential variations in lung cancer susceptibility related to radon progeny. Further, transcription of the cell-cycle regulatory gene Cip1 is regulated by p53 and increases following ionizing radiation. Therefore, variations in the expression of Cip1 following {alpha}-particle exposure may also be a susceptibility factor in radon-related lung cancers. The purpose of the present investigation was to measure p53 and Cip1 protein induction following {alpha}-particle exposure of fibroblast lines from nine individuals to determine if there were significant variations. The expression of Cip1 protein indicates the differences in response are biologically relevant.

  19. Whole-Genome Sequence of Corynebacterium auriscanis Strain CIP 106629 Isolated from a Dog with Bilateral Otitis from the United Kingdom.

    PubMed

    Tiwari, Sandeep; Jamal, Syed Babar; Oliveira, Leticia Castro; Clermont, Dominique; Bizet, Chantal; Mariano, Diego; de Carvalho, Paulo Vinicius Sanches Daltro; Souza, Flavia; Pereira, Felipe Luiz; de Castro Soares, Siomar; Guimarães, Luis C; Dorella, Fernanda; Carvalho, Alex; Leal, Carlos; Barh, Debmalya; Figueiredo, Henrique; Hassan, Syed Shah; Azevedo, Vasco; Silva, Artur

    2016-01-01

    In this work, we describe a set of features of Corynebacterium auriscanis CIP 106629 and details of the draft genome sequence and annotation. The genome comprises a 2.5-Mbp-long single circular genome with 1,797 protein-coding genes, 5 rRNA, 50 tRNA, and 403 pseudogenes, with a G+C content of 58.50%. PMID:27516502

  20. Whole-Genome Sequence of Corynebacterium auriscanis Strain CIP 106629 Isolated from a Dog with Bilateral Otitis from the United Kingdom

    PubMed Central

    Tiwari, Sandeep; Jamal, Syed Babar; Oliveira, Leticia Castro; Clermont, Dominique; Bizet, Chantal; Mariano, Diego; de Carvalho, Paulo Vinicius Sanches Daltro; Souza, Flavia; Pereira, Felipe Luiz; de Castro Soares, Siomar; Dorella, Fernanda; Carvalho, Alex; Leal, Carlos; Barh, Debmalya; Figueiredo, Henrique; Hassan, Syed Shah; Azevedo, Vasco

    2016-01-01

    In this work, we describe a set of features of Corynebacterium auriscanis CIP 106629 and details of the draft genome sequence and annotation. The genome comprises a 2.5-Mbp-long single circular genome with 1,797 protein-coding genes, 5 rRNA, 50 tRNA, and 403 pseudogenes, with a G+C content of 58.50%. PMID:27516502

  1. CASL L1 Milestone report : CASL.P4.01, sensitivity and uncertainty analysis for CIPS with VIPRE-W and BOA.

    SciTech Connect

    Sung, Yixing; Adams, Brian M.; Secker, Jeffrey R.

    2011-12-01

    The CASL Level 1 Milestone CASL.P4.01, successfully completed in December 2011, aimed to 'conduct, using methodologies integrated into VERA, a detailed sensitivity analysis and uncertainty quantification of a crud-relevant problem with baseline VERA capabilities (ANC/VIPRE-W/BOA).' The VUQ focus area led this effort, in partnership with AMA, and with support from VRI. DAKOTA was coupled to existing VIPRE-W thermal-hydraulics and BOA crud/boron deposit simulations representing a pressurized water reactor (PWR) that previously experienced crud-induced power shift (CIPS). This work supports understanding of CIPS by exploring the sensitivity and uncertainty in BOA outputs with respect to uncertain operating and model parameters. This report summarizes work coupling the software tools, characterizing uncertainties, and analyzing the results of iterative sensitivity and uncertainty studies. These studies focused on sensitivity and uncertainty of CIPS indicators calculated by the current version of the BOA code used in the industry. Challenges with this kind of analysis are identified to inform follow-on research goals and VERA development targeting crud-related challenge problems.

  2. Consolidation therapy with idarubicin, cisplatin and prednisone (CIP) after P-VABEC regimen in the treatment of intermediate and high grade non-Hodgkin's lymphoma of the elderly.

    PubMed

    Caracciolo, F; Capochiani, E; Papineschi, F; Petrini, M

    1997-01-01

    Aggressive treatments in elderly patients with NHL are often responsible for acute complications and increased mortality. The present study confirms that P-VABEC is able to induce a high CR rate (71%), with an overall response rate of 92%. The 4-year actuarial OS was 45%, and the FFS was 38%. Despite these good results 57% of CRs relapsed in a relatively short time (median 9.5 months; range 2-47). Because of this we decided to evaluate the role of a consolidation schedule (CIP), including idarubicin and cisplatin. The toxicity of P-VABEC/CIP regimen was comparable to that of P-VABEC alone. After a median follow-up of 20 months (range 8-49), 93% of CR patients treated with P-VABEC-CIP were still in complete remission. The 4-year actuarial overall survival was 92%, and the failure-free survival in CR patients was 72%. The difference in OS and FFS between the two groups was statistically significant. These results suggest that a short course of additional therapy is feasible in elderly patients treated with P-VABEC and may increase the OS and FFS, without adding toxicity. PMID:9156666

  3. Cdk5 inhibitory peptide (CIP) inhibits Cdk5/p25 activity induced by high glucose in pancreatic beta cells and recovers insulin secretion from p25 damage.

    PubMed

    Zheng, Ya-Li; Li, Congyu; Hu, Ya-Fang; Cao, Li; Wang, Hui; Li, Bo; Lu, Xiao-Hua; Bao, Li; Luo, Hong-Yan; Shukla, Varsha; Amin, Niranjana D; Pant, Harish C

    2013-01-01

    Cdk5/p25 hyperactivity has been demonstrated to lead to neuron apoptosis and degenerations. Chronic exposure to high glucose (HG) results in hyperactivity of Cdk5 and reduced insulin secretion. Here, we set out to determine whether abnormal upregulation of Cdk5/p25 activity may be induced in a pancreatic beta cell line, Min6 cells. We first confirmed that p25 were induced in overexpressed p35 cells treated with HG and increased time course dependence. Next, we showed that no p25 was detected under short time HG stimulation (4-12 hrs), however was detectable in the long exposure in HG cells (24 hrs and 48 hrs). Cdk5 activity in the above cells was much higher than low glucose treated cells and resulted in more than 50% inhibition of insulin secretion. We confirmed these results by overexpression of p25 in Min6 cells. As in cortical neurons, CIP, a small peptide, inhibited Cdk5/p25 activity and restored insulin secretion. The same results were detected in co-infection of dominant negative Cdk5 (DNCdk5) with p25. CIP also reduced beta cells apoptosis induced by Cdk5/p25. These studies indicate that Cdk5/p25 hyperactivation deregulates insulin secretion and induces cell death in pancreatic beta cells and suggests that CIP may serve as a therapeutic agent for type 2 diabetes. PMID:24039692

  4. Deficiency of cyclin-dependent kinase inhibitors p21{sup Cip1} and p27{sup Kip1} accelerates atherogenesis in apolipoprotein E-deficient mice

    SciTech Connect

    Akyuerek, Levent M.; Boehm, Manfred; Olive, Michelle; Zhou, Alex-Xianghua; San, Hong; Nabel, Elizabeth G.

    2010-05-28

    Cyclin-dependent kinase inhibitors, p21{sup Cip1} and p27{sup Kip1}, are upregulated during vascular cell proliferation and negatively regulate growth of vascular cells. We hypothesized that absence of either p21{sup Cip1} or p27{sup Kip1} in apolipoprotein E (apoE)-deficiency may increase atherosclerotic plaque formation. Compared to apoE{sup -/-} aortae, both apoE{sup -/-}/p21{sup -/-} and apoE{sup -/-}/p27{sup -/-} aortae exhibited significantly more atherosclerotic plaque following a high-cholesterol regimen. This increase was particularly observed in the abdominal aortic regions. Deficiency of p27{sup Kip1} accelerated plaque formation significantly more than p21{sup -/-} in apoE{sup -/-} mice. This increased plaque formation was in parallel with increased intima/media area ratios. Deficiency of p21{sup Cip1} and p27{sup Kip1} accelerates atherogenesis in apoE{sup -/-} mice. These findings have significant implications for our understanding of the molecular basis of atherosclerosis associated with excessive proliferation of vascular cells.

  5. Oridonin inhibits gefitinib-resistant lung cancer cells by suppressing EGFR/ERK/MMP-12 and CIP2A/Akt signaling pathways.

    PubMed

    Xiao, Xiangling; He, Zhongwei; Cao, Wei; Cai, Fen; Zhang, Liang; Huang, Qiuyue; Fan, Chunsheng; Duan, Chao; Wang, Xiaobo; Wang, Jiu; Liu, Ying

    2016-06-01

    Oridonin (Ori), a diterpenoid compound extracted from traditional medicinal herbs, elicits antitumor effects on many cancer types. However, whether Ori can be used in gefitinib-resistant non-small cell lung cancer (NSCLC) cells remains unclear. This study investigated the antitumor activity and underlying mechanisms of Ori. Results demonstrated that this compound dose-dependently inhibited the proliferation, invasion, and migration of the gefitinib-resistant NSCLC cells in vitro. Ori also significantly downregulated the phosphorylation of EGFR, ERK, Akt, expression levels of matrix metalloproteinase-12 (MMP-12), and the cancerous inhibitor of protein phosphatase 2A (CIP2A). In addition, Ori upregulated protein phosphatase 2A (PP2A) activity of gefitinib-resistant NSCLC cells. Ori combined with docetaxel synergistically inhibited these cells. Ori also inhibited tumor growth in murine models. Immunohistochemistry results further revealed that Ori downregulated phospho-EGFR, MMP-12, and CIP2A in vivo. These findings indicated that Ori can inhibit the proliferation, invasion, and migration of gefitinib-resistant NSCLC cells by suppressing EGFR/ERK/MMP-12 and CIP2A/PP2A/Akt signaling pathways. Thus, Ori may be a novel effective candidate to treat gefitinib-resistant NSCLC. PMID:27082429

  6. Multivariate statistical monitoring as applied to clean-in-place (CIP) and steam-in-place (SIP) operations in biopharmaceutical manufacturing.

    PubMed

    Roy, Kevin; Undey, Cenk; Mistretta, Thomas; Naugle, Gregory; Sodhi, Manbir

    2014-01-01

    Multivariate statistical process monitoring (MSPM) is becoming increasingly utilized to further enhance process monitoring in the biopharmaceutical industry. MSPM can play a critical role when there are many measurements and these measurements are highly correlated, as is typical for many biopharmaceutical operations. Specifically, for processes such as cleaning-in-place (CIP) and steaming-in-place (SIP, also known as sterilization-in-place), control systems typically oversee the execution of the cycles, and verification of the outcome is based on offline assays. These offline assays add to delays and corrective actions may require additional setup times. Moreover, this conventional approach does not take interactive effects of process variables into account and cycle optimization opportunities as well as salient trends in the process may be missed. Therefore, more proactive and holistic online continued verification approaches are desirable. This article demonstrates the application of real-time MSPM to processes such as CIP and SIP with industrial examples. The proposed approach has significant potential for facilitating enhanced continuous verification, improved process understanding, abnormal situation detection, and predictive monitoring, as applied to CIP and SIP operations. PMID:24532460

  7. Superintendent's Recommended FY 2014 Capital Budget and Amendments to the FY 2013-2018 Capital Improvements Program

    ERIC Educational Resources Information Center

    Montgomery County Public Schools, 2012

    2012-01-01

    In November 1996, the voters of Montgomery County (Maryland) approved by referendum an amendment to the County Charter that changed the County Council's review and approval cycle of the six-year Capital Improvements Program (CIP) from an annual to biennial cycle. The referendum specified that in odd-numbered fiscal years (on years) the County…

  8. FY 2014 Educational Facilities Master Plan and Amendments to the FY 2013-2018 Capital Improvements Program

    ERIC Educational Resources Information Center

    Montgomery County Public Schools, 2013

    2013-01-01

    In November 1996, the voters of Montgomery County (Maryland) approved by referendum an amendment to the County Charter that changed the County Council's review and approval cycle of the six-year Capital Improvements Program (CIP) from an annual to biennial cycle. The referendum specified that in odd-numbered fiscal years (on-years) the County…

  9. Superintendent's Recommended FY 2012 Capital Budget and Amendments to the FY 2011-2016 Capital Improvements Program

    ERIC Educational Resources Information Center

    Montgomery County Public Schools, 2010

    2010-01-01

    In November 1996, the voters of Montgomery County (Maryland) approved by referendum an amendment to the County Charter that changed the County Council's review and approval cycle of the six-year Capital Improvements Program (CIP) from an annual to biennial cycle. The referendum specified that in odd-numbered fiscal years (on-years) the County…

  10. IMAGES: A digital computer program for interactive modal analysis and gain estimation for eigensystem synthesis

    NASA Technical Reports Server (NTRS)

    Jones, R. L.

    1984-01-01

    An interactive digital computer program for modal analysis and gain estimation for eigensystem synthesis was written. Both mathematical and operation considerations are described; however, the mathematical presentation is limited to those concepts essential to the operational capability of the program. The program is capable of both modal and spectral synthesis of multi-input control systems. It is user friendly, has scratchpad capability and dynamic memory, and can be used to design either state or output feedback systems.

  11. National Land Use Monitoring Program in Multi-Temporal Remote Sensing Images

    NASA Astrophysics Data System (ADS)

    Kuo, YaoCheng; Chen, ChiFarn

    2016-04-01

    Change detection allows direct observation of land surface at repetitive intervals and provides important applications in environment monitoring, damage assessment and so on. And Detecting clouds in satellite imagery is becoming more important with increasing data availability, however many earth observation sensors are not designed for this task. Image radiometric normalization has caused problems in previous satellite image change and cloud detection methods is a precondition for deriving land change information of satellite imagery of different dates. To solve the ambiguity in using such resources, this paper proposes a complete change detection method based on iterative histogram and Fuzzy C-Mean which can cope with radiometric normalization, cloud and change detection. It firstly uses Fuzzy C-Mean clustering to dilute the small brightness area even with noise and outliers. And setting a different threshold at each band to find the cloud area. Then iterative algorithm applies the spectrum of invariant area for whole image with histogram matching. A threshold is then applied to difference images between the reference and the matched histogram subject images in order to detect the change areas. The image differentiation and threshold are again applied the updated histogram-matched image to further segment the change and the non-change areas. The creative parts of our approach lie in a three-step detection scheme is proved to be an efficient and effective way to differentiate the change area.

  12. Development of an imaging-planning program for screen/film and computed radiography mammography for breasts with short chest wall to nipple distance

    PubMed Central

    Dong, S L; Su, J L; Yeh, Y H; Chu, T C; Lin, Y C; Chuang, K S

    2011-01-01

    Objective Imaging breasts with a short chest wall to nipple distance (CWND) using a traditional mammographic X-ray unit is a technical challenge for mammographers. The purpose of this study is the development of an imaging-planning program to assist in determination of imaging parameters of screen/film (SF) and computed radiography (CR) mammography for short CWND breasts. Methods A traditional mammographic X-ray unit (Mammomat 3000, Siemens, Munich, Germany) was employed. The imaging-planning program was developed by combining the compressed breast thickness correction, the equivalent polymethylmethacrylate thickness assessment for breasts and the tube loading (mAs) measurement. Both phantom exposures and a total of 597 exposures were used for examining the imaging-planning program. Results Results of the phantom study show that the tube loading rapidly decreased with the CWND when the automatic exposure control (AEC) detector was not fully covered by the phantom. For patient exposures with the AEC fully covered by breast tissue, the average fractional tube loadings, defined as the ratio of the predicted mAs using the imaging-planning program and mAs of the mammogram, were 1.10 and 1.07 for SF and CR mammograms, respectively. The predicted mAs values were comparable to the mAs values, as determined by the AEC. Conclusion By applying the imaging-planning program in clinical practice, the experiential dependence of the mammographer for determination of the imaging parameters for short CWND breasts is minimised. PMID:21123310

  13. Anatomically Based Geometric Modelling Using Medical Image Data: Methods and Programs

    PubMed Central

    Wang, Monan; Sun, Lei; Liu, Yuming

    2015-01-01

    The human organs geometric modeling software which can achieve two-dimensional medical image browsing, pretreatment and three dimensional (3D) reconstruction in this paper is designed. This software implements medical image segmentation using the method combining the region growing and the interactive segmentation. Also, the MC surface reconstruction algorithm is utilized to achieve the three-dimensional reconstruction. Furthermore, the software is projected by Visual C++. And then, to legitimately express the structural information of skeleton and muscle, the software is employed to obtain the geometric model using the segmentation and three-dimensional reconstruction for data of skeleton and muscle medical images of the object of study. PMID:26089991

  14. Image

    Energy Science and Technology Software Center (ESTSC)

    2007-08-31

    The computer side of the IMAGE project consists of a collection of Perl scripts that perform a variety of tasks; scripts are available to insert, update and delete data from the underlying Oracle database, download data from NCBI's Genbank and other sources, and generate data files for download by interested parties. Web scripts make up the tracking interface, and various tools available on the project web-site (image.llnl.gov) that provide a search interface to the database.

  15. Moving from Efficacy to Effectiveness in Eating Disorders Prevention: The Sorority Body Image Program

    ERIC Educational Resources Information Center

    Becker, Carolyn Black; Ciao, Anna C.; Smith, Lisa M.

    2008-01-01

    Although eating disorders prevention research has begun to produce programs with demonstrated efficacy, many such programs simply target individuals as opposed to engaging broader social systems (e.g., schools, sororities, athletic teams) as participant collaborators in eating disorders prevention. Yet, social systems ultimately will be…

  16. Image detection and separation on Tautenburg Schmidt plates with the ASAG program package

    NASA Astrophysics Data System (ADS)

    Lange, M.; Richter, G. M.; Scholz, R.-D.

    1991-05-01

    The Babelsberg image processing system which will be used for the new automated measuring machine ASAG (Astronomisches Auswertegerat) includes as special feature an adaptive filtering technique. This technique allows a reduction of noise and the determination of the background in astronomical pictures and has already been used for the surface photometry of galaxies on Schmidt plates. For astrometric purposes a method of detecting and separating images in two-dimensional measuring rasters was developed within the frame of the ASAG software and algorithms.

  17. Doxorubicin Activates Hepatitis B Virus Replication by Elevation of p21 (Waf1/Cip1) and C/EBPα Expression

    PubMed Central

    Chen, Yu-Fang; Chong, Chin-Liew; Wu, Yi-Chieh; Wang, Yi-Ling; Tsai, Kuen-Nan; Kuo, Tzer-Min; Hong, Ming-Hsiang; Hu, Cheng-po; Chen, Mong-Liang; Chou, Yu-Chi; Chang, Chungming

    2015-01-01

    Hepatitis B virus reactivation is an important medical issue in cancer patients who undergo systemic chemotherapy. Up to half of CHB carriers receiving chemotherapy develop hepatitis and among these cases a notable proportion are associated with HBV reactivation. However, the molecular mechanism(s) through which various chemotherapeutic agents induce HBV reactivation is not yet fully understood. In this study, we investigated the role of the cell cycle regulator p21 (Waf1/Cip1) in the modulation of HBV replication when a common chemotherapeutic agent, doxorubicin, is present. We showed that p21 expression was increased by doxorubicin treatment. This elevation in p21 expression enhanced the expression of CCAAT/enhancer-binding protein α (C/EBPα); such an increase is likely to promote the binding of C/EBPα to the HBV promoter, which will contribute to the activation of HBV replication. Our current study thus reveals the mechanism underlying doxorubicin modulation of HBV replication and provides an increased understanding of HBV reactivation in CHB patients who are receiving systemic chemotherapy. PMID:26121644

  18. HERO: Program Status and Fist Images from a Balloon-Borne Focusing Hard-X-ray Telescope

    NASA Technical Reports Server (NTRS)

    Ramsey, B. D.; Alexander, C. D.; Apple, J. A.; Benson, C. M.; Dietz, K. L.; Elsner, R. F.; Engelhaupt. D. E.; Ghosh, K. K.; Kolodziejczak, J. J.; ODell, S. L.; Six, N. Frank (Technical Monitor)

    2001-01-01

    HERO is a balloon payload featuring shallow-graze angle replicated optics for hard-x-ray imaging. When completed, the instrument will offer unprecedented sensitivity in the hard-x-ray region, giving thousands of sources to choose from for detailed study on long flights. A recent proof-of-concept flight captured the first hard-x-ray focused images of the Crab Nebula, Cygnus X-1 and GRS 1915+105. Full details of the HERO program are presented, including the design and performance of the optics, the detectors and the gondola. Results from the recent proving flight are discussed together with expected future performance when the full science payload is completed.

  19. Actinic imaging of native and programmed defects on a full-field mask

    SciTech Connect

    Mochi, I.; Goldberg, K. A.; Fontaine, B. La; Tchikoulaeva, A.; Holfeld, C.

    2010-03-12

    We describe the imaging and characterization of native defects on a full field extreme ultraviolet (EUV) mask, using several reticle and wafer inspection modes. Mask defect images recorded with the SEMA TECH Berkeley Actinic Inspection Tool (AIT), an EUV-wavelength (13.4 nm) actinic microscope, are compared with mask and printed-wafer images collected with scanning electron microscopy (SEM) and deep ultraviolet (DUV) inspection tools. We observed that defects that appear to be opaque in the SEM can be highly transparent to EUV light, and inversely, defects that are mostly transparent to the SEM can be highly opaque to EUV. The nature and composition of these defects, whether they appear on the top surface, within the multilayer coating, or on the substrate as buried bumps or pits, influences both their significance when printed, and their detectability with the available techniques. Actinic inspection quantitatively predicts the characteristics of printed defect images in ways that may not be possible with non-EUV techniques. As a quantitative example, we investigate the main structural characteristics of a buried pit defect based on EUV through-focus imaging.

  20. Image dissector photocathode solar damage test program. [solar radiation shielding using a fast optical lens

    NASA Technical Reports Server (NTRS)

    Smith, R. A.

    1977-01-01

    Image dissector sensors of the same type which will be used in the NASA shuttle star tracker were used in a series of tests directed towards obtaining solar radiation/time damage criteria. Data were evaluated to determine the predicted level of operability of the star tracker if tube damage became a reality. During the test series a technique for reducing the solar damage effect was conceived and verified. The damage concepts are outlined and the test methods and data obtained which were used for verification of the technique's feasibility are presented. The ability to operate an image dissector sensor with the solar image focussed on the photocathode by a fast optical lens under certain conditions is feasible and the elimination of a mechanical protection device is possible.

  1. Transcriptional inhibition of p21WAF1/CIP1 gene (CDKN1) expression by survivin is at least partially p53-dependent: evidence for survivin acting as a transcription factor or co-factor.

    PubMed

    Tang, Lei; Ling, Xiang; Liu, Wensheng; Das, Gokul M; Li, Fengzhi

    2012-05-01

    Growing evidence suggests a role for the antiapoptotic protein survivin in promotion of cancer cell G1/S transition and proliferation. However, the underlying mechanism is unclear. Further, although upregulation of p21(WAF1/CIP1) by p53 plays an important role in p53-mediated cell G1 arrests in response to various distresses, it is unknown whether survivin plays a role in the regulation of p21(WAF1/CIP1) expression. Here, we report that exogenous expression of survivin in p53-wild type MCF-7 breast cancer cells inhibits the expression of p21(WAF1/CIP1) protein, mRNA and promoter activity, while the survivin C84A mutant and antisense failed to do so. Cotransfection experiments in the p53 mutant H1650 lung cancer cell line showed that survivin neutralizes p53-induced p21(WAF1/CIP1) expression and promoter activity. Importantly, genetically silencing of endogenous survivin using lentiviral survivin shRNA also enhances endogenous p21 in p53 wild type cancer cells, suggesting the physiological relevance of the fining. We further demonstrated that both p53 and survivin interacts on the two p53-binding sites in the p21(WAF1/CIP1) promoter (-2313 to -2212; -1452 to -1310), and survivin physically interacts with p53 in cancer cells. Together, we propose that survivin may act as a transcription factor or cofactor to interact with p53 on the p21(WAF1/CIP1) promoter leading to the inhibition of p21(WAF1/CIP1) expression at least in part by neutralizing p53-mediated transcriptional activation of the p21 gene. PMID:22503977

  2. MRI Mode Programming for Safe Magnetic Resonance Imaging in Patients With a Magnetic Resonance Conditional Cardiac Device.

    PubMed

    Nakai, Toshiko; Kurokawa, Sayaka; Ikeya, Yukitoshi; Iso, Kazuki; Takahashi, Keiko; Sasaki, Naoko; Ashino, Sonoko; Okubo, Kimie; Okumura, Yasuo; Kunimoto, Satoshi; Watanabe, Ichiro; Hirayama, Atsushi

    2016-01-01

    Although diagnostically indispensable, magnetic resonance imaging (MRI) has been, until recently, contraindicated in patients with an implantable cardiac device. MR conditional cardiac devices are now widely used, but the mode programming needed for safe MRI has yet to be established. We reviewed the details of 41 MRI examinations of patients with a MR conditional device. There were no associated adverse events. However, in 3 cases, paced beats competed with the patient's own beats during the MRI examination. We describe 2 of the 3 specific cases because they illustrate these potentially risky situations: a case in which the intrinsic heart rate increased and another in which atrial fibrillation occurred. Safe MRI in patients with an MR conditional device necessitates detailed MRI mode programming. The MRI pacing mode should be carefully and individually selected. PMID:26973263

  3. Images.

    ERIC Educational Resources Information Center

    Barr, Catherine, Ed.

    1997-01-01

    The theme of this month's issue is "Images"--from early paintings and statuary to computer-generated design. Resources on the theme include Web sites, CD-ROMs and software, videos, books, and others. A page of reproducible activities is also provided. Features include photojournalism, inspirational Web sites, art history, pop art, and myths. (AEF)

  4. Incorporating the whole-mount prostate histology reconstruction program Histostitcher into the extensible imaging platform (XIP) framework

    NASA Astrophysics Data System (ADS)

    Toth, Robert; Chappelow, Jonathan; Vetter, Christoph; Kutter, Oliver; Russ, Christoph; Feldman, Michael; Tomaszewski, John; Shih, Natalie; Madabhushi, Anant

    2012-03-01

    There is a need for identifying quantitative imaging (e.g. MRI) signatures for prostate cancer (CaP), so that computer-aided diagnostic methods can be trained to detect disease extent in vivo. Determining CaP extent on in vivo MRI is difficult to do; however, with the availability of ex vivo surgical whole mount histological sections (WMHS) for CaP patients undergoing radical prostatectomy, co-registration methods can be applied to align and map disease extent onto pre-operative MR imaging from the post-operative histology. Yet obtaining digitized images of WHMS for co-registration with the pre-operative MRI is cumbersome since (a) most digital slide scanners are unable to accommodate the entire section, and (b) significant technical expertise is required for whole mount slide preparation. Consequently, most centers opt to construct quartered sections of each histology slice. Prior to co-registration with MRI, however, these quartered sections need to be digitally stitched together to reconstitute a digital, pseudo WMHS. Histostitcheris an interactive software program that uses semi-automatic registration tools to digitally stitch quartered sections into pseudo WMHS. Histostitcherwas originally developed using the GUI tools provided by the Matlab programming interface, but the clinical use was limited due to the inefficiency of the interface. The limitations of the Matlab based GUI include (a) an inability to edit the fiducials, (b) the rendering being extremely slow, and (c) lack of interactive and rapid visualization tools. In this work, Histostitcherhas been integrated into the eXtensible Imaging Platform (XIP TM ) framework (a set of libraries containing functionalities for analyzing and visualizing medical image data). XIP TM lends the stitching tool much greater flexibility and functionality by (a) allowing interactive and seamless navigation through the full resolution histology images, (b) the ability to easily add, edit, or remove fiducials and annotations

  5. Overview of NASARTI (NASA Radiation Track Image) Program: Highlights of the Model Improvement and the New Results

    NASA Technical Reports Server (NTRS)

    Ponomarev, Artem L.; Plante, I.; George, Kerry; Cornforth, M. N.; Loucas, B. D.; Wu, Honglu

    2014-01-01

    This presentation summarizes several years of research done by the co-authors developing the NASARTI (NASA Radiation Track Image) program and supporting it with scientific data. The goal of the program is to support NASA mission to achieve a safe space travel for humans despite the perils of space radiation. The program focuses on selected topics in radiation biology that were deemed important throughout this period of time, both for the NASA human space flight program and to academic radiation research. Besides scientific support to develop strategies protecting humans against an exposure to deep space radiation during space missions, and understanding health effects from space radiation on astronauts, other important ramifications of the ionizing radiation were studied with the applicability to greater human needs: understanding the origins of cancer, the impact on human genome, and the application of computer technology to biological research addressing the health of general population. The models under NASARTI project include: the general properties of ionizing radiation, such as particular track structure, the effects of radiation on human DNA, visualization and the statistical properties of DSBs (DNA double-strand breaks), DNA damage and repair pathways models and cell phenotypes, chromosomal aberrations, microscopy data analysis and the application to human tissue damage and cancer models. The development of the GUI and the interactive website, as deliverables to NASA operations teams and tools for a broader research community, is discussed. Most recent findings in the area of chromosomal aberrations and the application of the stochastic track structure are also presented.

  6. Heart imaging by cadmium telluride gamma cameraEuropean Program ``BIOMED'' consortium

    NASA Astrophysics Data System (ADS)

    Scheiber, Ch.; Eclancher, B.; Chambron, J.; Prat, V.; Kazandjan, A.; Jahnke, A.; Matz, R.; Thomas, S.; Warren, S.; Hage-Hali, M.; Regal, R.; Siffert, P.; Karman, M.

    1999-06-01

    Cadmium telluride semiconductor detectors (CdTe) operating at room temperature are attractive for medical imaging because of their good energy resolution providing excellent spatial and contrast resolution. The compactness of the detection system allows the building of small light camera heads which can be used for bedside imaging. A mobile pixellated gamma camera based on 2304 CdTe (pixel size: 3×3 mm, field of view: 15 cm×15 cm) has been designed for cardiac imaging. A dedicated 16-channel integrated circuit has also been designed. The acquisition hardware is fully programmable (DSP card, personal computer-based system). Analytical calculations have shown that a commercial parrallel hole collimator will fit the efficiency/resolution requirements for cardiac applications. Monte-Carlo simulations predict that the Moire effect can be reduced by a 15° tilt of the collimator with respect to the detector grid. A 16×16 CdTe module has been built for the preliminary physical tests. The energy resolution was 6.16±0.6 keV (mean ± standard deviation, n=30). Uniformity was ±10%, improving to ±1% when using a correction table. Test objects (emission data: letters 1.8 mm in width) and cold rods in scatter medium have been acquired. The CdTe images have been compared to those acquired with a conventionnal gamma camera.

  7. A Positive Self Image. It's Your Choice. A Classroom Program for the Development of Self Esteem.

    ERIC Educational Resources Information Center

    Hanel, Robert R.

    Arranged into two parts, the book provides a model for helping students develop a positive self-esteem. Part 1 presents five lessons aimed at improving self-image. Lesson 1 focuses on the relationship between one's self-worth and one's behavior. Lesson 2 deals with appearance and the notion that body characteristics may change one's value as a…

  8. The Convergence of Information Technology, Data, and Management in a Library Imaging Program

    ERIC Educational Resources Information Center

    France, Fenella G.; Emery, Doug; Toth, Michael B.

    2010-01-01

    Integrating advanced imaging and processing capabilities in libraries, archives, and museums requires effective systems and information management to ensure that the large amounts of digital data about cultural artifacts can be readily acquired, stored, archived, accessed, processed, and linked to other data. The Library of Congress is developing…

  9. Seasonal change detection of riparian zones with remote sensing images and genetic programming in a semi-arid watershed.

    PubMed

    Makkeasorn, Ammarin; Chang, Ni-Bin; Li, Jiahong

    2009-02-01

    Riparian zones are deemed significant due to their interception capability of non-point source impacts and the maintenance of ecosystem integrity region wide. To improve classification and change detection of riparian buffers, this paper developed an evolutionary computational, supervised classification method--the RIparian Classification Algorithm (RICAL)--to conduct the seasonal change detection of riparian zones in a vast semi-arid watershed, South Texas. RICAL uniquely demonstrates an integrative effort to incorporate both vegetation indices and soil moisture images derived from LANDSAT 5 TM and RADARSAT-1 satellite images, respectively. First, an estimation of soil moisture based on RADARSAT-1 Synthetic Aperture Radar (SAR) images was conducted via the first-stage genetic programming (GP) practice. Second, for the statistical analyses and image classification, eight vegetation indices were prepared based on reflectance factors that were calculated as the response of the instrument on LANDSAT. These spectral vegetation indices were then independently used for discriminate analysis along with soil moisture images to classify the riparian zones via the second-stage GP practice. The practical implementation was assessed by a case study in the Choke Canyon Reservoir Watershed (CCRW), South Texas, which is mostly agricultural and range land in a semi-arid coastal environment. To enhance the application potential, a combination of Iterative Self-Organizing Data Analysis Techniques (ISODATA) and maximum likelihood supervised classification was also performed for spectral discrimination and classification of riparian varieties comparatively. Research findings show that the RICAL algorithm may yield around 90% accuracy based on the unseen ground data. But using different vegetation indices would not significantly improve the final quality of the spectral discrimination and classification. Such practices may lead to the formulation of more effective management strategies

  10. Interactive multimedia program for imaging the spleen: concept, design, and development.

    PubMed

    Calhoun, P S; Fishman, E K

    1994-11-01

    The authors present some of the decision making and design goals that guided the development of computer-based educational programs, specifically a module to teach the role of computed tomography in detection and evaluation of splenic disease. One goal was that the program would take advantage of the computer yet allow users to recognize or learn quickly the techniques used to navigate through the program. A game interface was chosen as a means to aid retention of the material being taught, as well as to make use of the module enjoyable. The structure used to present the program content was based on the common means by which radiology has traditionally been taught. The four sections of the module consist of the slide lecture, traditional text, a quiz, and teaching files. Each question in the quiz section is linked to the relevant text section for review, and the teaching file section can be used as a self-test by hiding the text and diagnosis fields. Computer-based educational programs are an important resource for residents and practicing professionals, and they will continue to evolve with developments in technology. However, it should be the goal of all new projects to find a balance of form, in which the value of traditional teaching methods can be preserved with the maximum opportunity for innovation. PMID:7855349

  11. Suppression of cancer cell growth by adenovirus expressing p21(WAF1/CIP1) deficient in PCNA interaction.

    PubMed

    Prabhu, N S; Blagosklonny, M V; Zeng, Y X; Wu, G S; Waldman, T; El-Deiry, W S

    1996-07-01

    p53 tumor suppression is deficient in the majority of human cancers. Efforts to understand this pathway have identified cyclin-dependent kinase (CDK) inhibitors and suggested a potential for their replacement in human cancer. In the present studies, expression of a C-terminal deletion mutant of the human p21(WAF1/CIP1) CDK inhibitor completely suppressed the growth of colon cancer cells, whereas full-length p21 only partially suppressed growth. We prepared a replication-deficient adenoviral recombinant which expresses the p21 C-terminal mutant (Ad-WAF1-341) and compared its tumor suppressive abilities with Ad-p53 and Ad-LacZ. Ad-WAF1-341- and Ad-p53-infected cancer cells, but not Ad-LacZ-infected cancer cells, expressed a nuclear protein recognized by anti-p21 antibody and were deficient in cell cycle progression. The exogenous p21 mutant interacted with CDK2 but not proliferating cell nuclear antigen following infection of p21-/- cancer cells. Ad-WAF1-341 was more potent than Ad-p53 in inhibiting DNA synthesis in human papillomavirus 16 E6-expressing cancer cells. Most importantly, the Ad-WAF1-341-infected E6-expressing cells died, whereas most of the Ad-p53-infected cells continued to proliferate. Endonucleolytic cleavage of DNA was observed in Ad-WAF1-341-infected cancer cells. These observations suggest that Ad-WAF1-341 should be evaluated in the treatment of human papillomavirus-associated neoplasia and other neoplasias resistant to p53. PMID:9816291

  12. p21{sup WAF1/CIP1} deficiency induces mitochondrial dysfunction in HCT116 colon cancer cells

    SciTech Connect

    Kim, Ae Jeong; Jee, Hye Jin; Song, Naree; Kim, Minjee; Jeong, Seon-Young; Yun, Jeanho

    2013-01-11

    Highlights: Black-Right-Pointing-Pointer p21{sup -/-} HCT116 cells exhibited an increase in mitochondrial mass. Black-Right-Pointing-Pointer The expression levels of PGC-1{alpha} and AMPK were upregulated in p21{sup -/-} HCT116 cells. Black-Right-Pointing-Pointer The proliferation of p21{sup -/-} HCT116 cells in galactose medium was significantly impaired. Black-Right-Pointing-Pointer p21 may play a role in maintaining proper mitochondrial mass and respiratory function. -- Abstract: p21{sup WAF1/CIP1} is a critical regulator of cell cycle progression. However, the role of p21 in mitochondrial function remains poorly understood. In this study, we examined the effect of p21 deficiency on mitochondrial function in HCT116 human colon cancer cells. We found that there was a significant increase in the mitochondrial mass of p21{sup -/-} HCT116 cells, as measured by 10-N-nonyl-acridine orange staining, as well as an increase in the mitochondrial DNA content. In contrast, p53{sup -/-} cells had a mitochondrial mass comparable to that of wild-type HCT116 cells. In addition, the expression levels of the mitochondrial biogenesis regulators PGC-1{alpha} and TFAM and AMPK activity were also elevated in p21{sup -/-} cells, indicating that p21 deficiency induces the rate of mitochondrial biogenesis through the AMPK-PGC-1{alpha} axis. However, the increase in mitochondrial biogenesis in p21{sup -/-} cells did not accompany an increase in the cellular steady-state level of ATP. Furthermore, p21{sup -/-} cells exhibited significant proliferation impairment in galactose medium, suggesting that p21 deficiency induces a defect in the mitochondrial respiratory chain in HCT116 cells. Taken together, our results suggest that the loss of p21 results in an aberrant increase in the mitochondrial mass and in mitochondrial dysfunction in HCT116 cells, indicating that p21 is required to maintain proper mitochondrial mass and respiratory function.

  13. Validation of the analysis of respirable crystalline silica (quartz) in foams used with CIP 10-R samplers.

    PubMed

    Eypert-Blaison, Céline; Moulut, Jean-Claude; Lecaque, Thierry; Marc, Florian; Kauffer, Edmond

    2011-05-01

    Sampling the respirable fraction to measure exposure to crystalline silica is most often carried out using cyclones. However, low flow rates (<4 l min(-1)) and continuing improvement in workplace hygiene means less and less material is sampled for analysis, resulting in increased analytical uncertainty. Use of the CIP 10-R sampler, working at a flow rate of 10 l min(-1), is one attempt to solve current analytical difficulties. To check the ability of the analysis of quartz sampled on foams, known amounts of quartz associated with a matrix have been injected into foams. The results obtained show that the proposed protocol, with prior acid attack and ashing of the foams, satisfies the recommendations of EN 482 Standard [CEN. (2006) Workplace atmospheres-general requirements for the performance of procedures for the measurements of chemical agents. Brussels, Belgium: EN 482 Comité Européen de normalization (CEN).], namely an expanded uncertainty of <50% for quartz weights between 0.1 and 0.5 times the 8-h exposure limit value and <30% for quartz weights between 0.5 and 2 times the 8-h exposure limit value, assuming an exposure limit value equal to 0.1 mg m(-3). Results obtained show that the 101 reflection line allows a quartz quantity of the order of 25 μg to be satisfactorily measured, which corresponds to a 10th of the exposure limit value, assuming an exposure limit value of 0.05 mg m(-3). In this case, the 100 and 112 reflection lines with expanded uncertainties of ~50% would also probably lead to satisfactory quantification. Particular recommendations are also proposed for the preparation of calibration curves to improve the method. PMID:21257743

  14. Loss of p21WAF1/Cip1 in Gadd45-deficient keratinocytes restores DNA repair capacity.

    PubMed

    Maeda, Tomoko; Espino, Robin A; Chomey, Eugene G; Luong, Le; Bano, Ather; Meakins, Diana; Tron, Victor A

    2005-10-01

    Ultraviolet light (UV)-induced DNA damage is repaired primarily by the nucleotide excision repair (NER) pathway. Gadd45 is a multifunctional protein that regulates NER. Gadd45-deficient keratinocytes fail to repair UV-induced DNA damage, but the mechanism by which Gadd45 stimulates repair of UV-induced DNA damage is unknown. p21WAF1/Cip1 (p21) is a well-characterized downstream target of p53 that binds to Gadd45 and proliferating cell nuclear antigen (PCNA). The role of p21 in NER is somewhat controversial, however, recent studies appear to suggest that it inhibits DNA repair by inhibiting PCNA activity. Since a physical interplay exists between p21, Gadd45 and PCNA, we hypothesized that Gadd45 promoted DNA repair via p21. Initially, we examined p21 protein expression in Gadd45-deficient and proficient mice and found a higher base level of p21 protein in Gadd45-deficient keratinocytes and in most other tissues. With these results, we next speculated on the role played by p21 in Gadd45 regulated NER, by exposing keratinocytes from wild-type, single and double knockout (Gadd45 and p21) mice to UV, and measuring the responses. We confirmed that Gadd45-deficient keratinocytes were defective in UV-induced NER, but interestingly Gadd45/p21-null keratinocytes had normal NER in response to UV. Furthermore, Gadd45/p21-null keratinocytes were more resistant to UV-induced cell death than Gadd45-deficient keratinocytes. These results support the hypothesis that Gadd45 enhances NER by negatively regulating basal p21 expression in keratinocytes. PMID:15917306

  15. p21(WAF1) (/Cip1) limits senescence and acinar-to-ductal metaplasia formation during pancreatitis.

    PubMed

    Grabliauskaite, Kamile; Hehl, Adrian B; Seleznik, Gitta M; Saponara, Enrica; Schlesinger, Kathryn; Zuellig, Richard A; Dittmann, Anja; Bain, Martha; Reding, Theresia; Sonda, Sabrina; Graf, Rolf

    2015-02-01

    Trans-differentiation of pancreatic acinar cells into ductal-like lesions, a process defined as acinar-to-ductal metaplasia (ADM), is observed in the course of organ regeneration following pancreatitis. In addition, ADM is found in association with pre-malignant PanIN lesions and correlates with an increased risk of pancreatic adenocarcinoma (PDAC). Human PDAC samples show down-regulation of p21(WAF1) (/Cip1) , a key regulator of cell cycle and cell differentiation. Here we investigated whether p21 down-regulation is implicated in controlling the early events of acinar cell trans-differentiation and ADM formation. p21-mediated regulation of ADM formation and regression was analysed in vivo during the course of cerulein-induced pancreatitis, using wild-type (WT) and p21-deficient (p21(-/-) ) mice. Biochemical and immunohistochemical methods were used to evaluate disease progression over 2 weeks of the disease and during a recovery phase. We found that p21 was strongly up-regulated in WT acinar cells during pancreatitis, while it was absent in ADM areas, suggesting that p21 down-regulation is associated with ADM formation. In support of this hypothesis, p21(-/-) mice showed a significant increase in number and size of metaplasia. In addition, p21 over-expression in acinar cells reduced ADM formation in vitro, suggesting that the protein regulates the metaplastic transition in a cell-autonomous manner. p21(-/-) mice displayed increased expression and relocalization of β-catenin both during pancreatitis and in the subsequent recovery phase. Finally, loss of p21 was accompanied by increased DNA damage and development of senescence. Our findings are consistent with a gate-keeper role of p21 in acinar cells to limit senescence activation and ADM formation during pancreatic regeneration. PMID:25212177

  16. p21{sup WAF1/Cip1/Sdi1} knockout mice respond to doxorubicin with reduced cardiotoxicity

    SciTech Connect

    Terrand, Jerome; Xu, Beibei; Morrissy, Steve; Dinh, Thai Nho; Williams, Stuart; Chen, Qin M.

    2011-11-15

    Doxorubicin (Dox) is an antineoplastic agent that can cause cardiomyopathy in humans and experimental animals. As an inducer of reactive oxygen species and a DNA damaging agent, Dox causes elevated expression of p21{sup WAF1/Cip1/Sdi1} (p21) gene. Elevated levels of p21 mRNA and p21 protein have been detected in the myocardium of mice following Dox treatment. With chronic treatment of Dox, wild type (WT) animals develop cardiomyopathy evidenced by elongated nuclei, mitochondrial swelling, myofilamental disarray, reduced cardiac output, reduced ejection fraction, reduced left ventricular contractility, and elevated expression of ANF gene. In contrast, p21 knockout (p21KO) mice did not show significant changes in the same parameters in response to Dox treatment. In an effort to understand the mechanism of the resistance against Dox induced cardiomyopathy, we measured levels of antioxidant enzymes and found that p21KO mice did not contain elevated basal or inducible levels of glutathione peroxidase and catalase. Measurements of 6 circulating cytokines indicated elevation of IL-6, IL-12, IFN{gamma} and TNF{alpha} in Dox treated WT mice but not p21KO mice. Dox induced elevation of IL-6 mRNA was detected in the myocardium of WT mice but not p21KO mice. While the mechanism of the resistance against Dox induced cardiomyopathy remains unclear, lack of inflammatory response may contribute to the observed cardiac protection in p21KO mice. -- Highlights: Black-Right-Pointing-Pointer Doxorubicin induces p21 elevation in the myocardium. Black-Right-Pointing-Pointer Doxorubicin causes dilated cardiomyopathy in wild type mice. Black-Right-Pointing-Pointer p21 Knockout mice are resistant against doxorubicin induced cardiomyopathy. Black-Right-Pointing-Pointer Lack of inflammatory response correlates with the resistance in p21 knockout mice.

  17. Planning and Implementing Institutional Image and Promoting Academic Programs in Higher Education

    ERIC Educational Resources Information Center

    Cetin, Rubeena

    2003-01-01

    Universities face a multitude of issues and challenges in the current era of higher educational endeavors. Universities are being urged to provide high quality education, exist as a well-reputed university, achieve enrollment success, improve competitive positioning, provide contemporary and well-designed academic programs, and maintain financial…

  18. Designing a University-Wide Fitness Program to Promote Campus Health and Department Image

    ERIC Educational Resources Information Center

    VanSickle, Jennifer; Hancher-Rauch, Heidi; Hicks, Lisa

    2010-01-01

    Health and physical education departments across the United States often face perception and reputation problems on campus and a general misunderstanding of the nature of their academic work. One solution is to offer a faculty-and-staff fitness program that showcases the academic and leadership skills of the faculty, opens doors for collaboration…

  19. The masked educator-innovative simulation in an Australian undergraduate Medical Sonography and Medical Imaging program

    PubMed Central

    Reid-Searl, Kerry; Bowman, Anita; McAllister, Margaret; Cowling, Cynthia; Spuur, Kelly

    2014-01-01

    Introduction Clinical learning experiences for sonography and medical imaging students can sometimes involve the practice of technical procedures with less of a focus on developing communication skills with patients. Whilst patient-based simulation scenarios have been widely reported in other health education programmes, there is a paucity of research in sonography and medical imaging. The aim of this study was to explore the effectiveness of Mask-Ed™ (KRS Simulation) in the learning and teaching of clinical communication skills to undergraduate medical sonography and medical imaging students. Mask-Ed™ (KRS Simulation) is a simulation technique where the educator is hidden behind wearable realistic silicone body props including masks. Methods Focus group interviews were conducted with 11 undergraduate medical sonography and medical imaging students at CQUniversity, Australia. The number of participants was limited to the size of the cohort of students enrolled in the course. Prior to these interviews participants were engaged in learning activities that featured the use of the Mask-Ed™ (KRS Simulation) method. Thematic analysis was employed to explore how the introduction of Mask-Ed™ (KRS Simulation) contributed to students' learning in relation to clinical communication skills. Results Key themes included: benefits of interacting with someone real rather than another student, learning made fun, awareness of empathy, therapeutic communication skills, engaged problem solving and purposeful reflection. Conclusions Mask-Ed™ (KRS Simulation) combined with interactive sessions with an expert facilitator, contributed positively to students' learning in relation to clinical communication skills. Participants believed that interacting with someone real, as in the Mask-Ed characters was beneficial. In addition to the learning being described as fun, participants gained an awareness of empathy, therapeutic communication skills, engaged problem solving and

  20. Gender Related Differences in Response to “In Favor of Myself” Wellness Program to Enhance Positive Self & Body Image among Adolescents

    PubMed Central

    Golan, Moria; Hagay, Noa; Tamir, Snait

    2014-01-01

    Background Physical, neurological and psychological changes are often experienced differently by male and female adolescents. Positive self-esteem, emotional well-being, school achievements, and family connectedness are considered as protective factors against health-compromising behaviors. This study examines the gender differences in respect to the effect of a school-based interactive wellness program – “In Favor of Myself” – on self-image, body image, eating attitudes and behaviors of young adolescents. Methods Two hundred and ten adolescents (mean age 13.5) participated in the intervention group, 55% were girls and 45% boys. Program consisted of eight 90-minutes structured sessions integrated into a regular school coping skills curriculum. The program focused on self-esteem, self-image, body image, media literacy and cognitive dissonance. The overall impact of the program and the study protocol were previously published. Results Overall, there are gender related differences in respect to body image and self-image in young adolescents in response to “In Favor of Myself”. Compared to boys, girls reported at baseline higher self-esteem, being more contingent by appearance, and their self-image was more influenced by popularity, appearance, interpersonal communication and admired people. Furthermore girls presented greater gap between current body figure and perceived ideal figure. Not only were girls more dissatisfied with their body, but they were more active in attempts to become and/or remain “thin”. At program termination, gender × time effect was detected in reduction of self-worth contingent by others, change in importance given to achievements at schools, parents' perceptions, as well as the impact of comparisons to friends and family members on self-image. Conclusions Girls exhibited more gains than boys from ‘In Favor of Myself’ which raise the questions about how effective would be the program when delivered in mixed gender groups vs

  1. The infrared imaging spectrograph (IRIS) for TMT: overview of innovative science programs

    NASA Astrophysics Data System (ADS)

    Wright, Shelley A.; Larkin, James E.; Moore, Anna M.; Do, Tuan; Simard, Luc; Adamkovics, Maté; Armus, Lee; Barth, Aaron J.; Barton, Elizabeth; Boyce, Hope; Cooke, Jeffrey; Cote, Patrick; Davidge, Timothy; Ellerbroek, Brent; Ghez, Andrea M.; Liu, Michael C.; Lu, Jessica R.; Macintosh, Bruce A.; Mao, Shude; Marois, Christian; Schoeck, Matthias; Suzuki, Ryuji; Tan, Jonathan C.; Treu, Tommaso; Wang, Lianqi; Weiss, Jason

    2014-07-01

    IRIS (InfraRed Imaging Spectrograph) is a first light near-infrared diffraction limited imager and integral field spectrograph being designed for the future Thirty Meter Telescope (TMT). IRIS is optimized to perform astronomical studies across a significant fraction of cosmic time, from our Solar System to distant newly formed galaxies (Barton et al. [1]). We present a selection of the innovative science cases that are unique to IRIS in the era of upcoming space and ground-based telescopes. We focus on integral field spectroscopy of directly imaged exoplanet atmospheres, probing fundamental physics in the Galactic Center, measuring 104 to 1010 M supermassive black hole masses, resolved spectroscopy of young star-forming galaxies (1 < z < 5) and first light galaxies (6 < z < 12), and resolved spectroscopy of strong gravitational lensed sources to measure dark matter substructure. For each of these science cases we use the IRIS simulator (Wright et al. [2], Do et al. [3]) to explore IRIS capabilities. To highlight the unique IRIS capabilities, we also update the point and resolved source sensitivities for the integral field spectrograph (IFS) in all five broadband filters (Z, Y, J, H, K) for the finest spatial scale of 0.004" per spaxel. We briefly discuss future development plans for the data reduction pipeline and quicklook software for the IRIS instrument suite.

  2. Specific NIST projects in support of the NIJ Concealed Weapon Detection and Imaging Program

    NASA Astrophysics Data System (ADS)

    Paulter, Nicholas G.

    1998-12-01

    The Electricity Division of the National Institute of Standards and Technology is developing revised performance standards for hand-held (HH) and walk-through (WT) metal weapon detectors, test procedures and systems for these detectors, and a detection/imaging system for finding concealed weapons. The revised standards will replace the existing National Institute of Justice (NIJ) standards for HH and WT devices and will include detection performance specifications as well as system specifications (environmental conditions, mechanical strength and safety, response reproducibility and repeatability, quality assurance, test reporting, etc.). These system requirements were obtained from the Law Enforcement and corrections Technology Advisory Council, an advisory council for the NIJ. Reproducible and repeatable test procedures and appropriate measurement systems will be developed for evaluating HH and WT detection performance. A guide to the technology and application of non- eddy-current-based detection/imaging methods (such as acoustic, passive millimeter-wave and microwave, active millimeter-wave and terahertz-wave, x-ray, etc.) Will be developed. The Electricity Division is also researching the development of a high- frequency/high-speed (300 GH to 1 THz) pulse-illuminated, stand- off, video-rate, concealed weapons/contraband imaging system.

  3. A linear programming approach to reconstructing subcellular structures from confocal images for automated generation of representative 3D cellular models

    PubMed Central

    Wood, Scott T.; Dean, Brian C.; Dean, Delphine

    2013-01-01

    This paper presents a novel computer vision algorithm to analyze 3D stacks of confocal images of fluorescently stained single cells. The goal of the algorithm is to create representative in silico model structures that can be imported into finite element analysis software for mechanical characterization. Segmentation of cell and nucleus boundaries is accomplished via standard thresholding methods. Using novel linear programming methods, a representative actin stress fiber network is generated by computing a linear superposition of fibers having minimum discrepancy compared with an experimental 3D confocal image. Qualitative validation is performed through analysis of seven 3D confocal image stacks of adherent vascular smooth muscle cells (VSMCs) grown in 2D culture. The presented method is able to automatically generate 3D geometries of the cell's boundary, nucleus, and representative F-actin network based on standard cell microscopy data. These geometries can be used for direct importation and implementation in structural finite element models for analysis of the mechanics of a single cell to potentially speed discoveries in the fields of regenerative medicine, mechanobiology, and drug discovery. PMID:23395283

  4. Optical Technologies and Molecular Imaging for Cervical Neoplasia: A Program Project Update

    PubMed Central

    Buys, Timon P. H.; Cantor, Scott B.; Guillaud, Martial; Adler-Storthz, Karen; Cox, Dennis D.; Okolo, Clement; Arulogon, Oyedunni; Oladepo, Oladimeji; Basen-Engquist, Karen; Shinn, Eileen; Yamal, José-Miguel; Beck, J. Robert; Scheurer, Michael E.; van Niekerk, Dirk; Malpica, Anais; Matisic, Jasenka; Staerkel, Gregg; Atkinson, Edward Neely; Bidaut, Luc; Lane, Pierre; Benedet, J. Lou; Miller, Dianne; Ehlen, Tom; Price, Roderick; Adewole, Isaac F.; MacAulay, Calum; Follen, Michele

    2011-01-01

    There is an urgent global need for effective and affordable approaches to cervical cancer screening and diagnosis. For developing nations, cervical malignancies remain the leading cause of cancer death in women. This reality is difficult to accept given that these deaths are largely preventable; where cervical screening programs are implemented, cervical cancer deaths decrease dramatically. In the developed world, the challenges with respect to cervical disease stem from high costs and over-treatment. We are presently eleven years into a National Cancer Institute-funded Program Project (P01 CA82710) that is evaluating optical technologies for their applicability to the cervical cancer problem. Our mandate is to create new tools for disease detection and diagnosis that are inexpensive, require minimal expertise to use, are more accurate than existing modalities, and will be feasibly implemented in a variety of clinical settings. Herein, we update the status of this work and explain the long-term goals of this project. PMID:21944317

  5. Overexpression of caspase 7 is ERα dependent to affect proliferation and cell growth in breast cancer cells by targeting p21Cip

    PubMed Central

    Chaudhary, S; Madhukrishna, B; Adhya, A K; Keshari, S; Mishra, S K

    2016-01-01

    Caspase 7 (CASP7) expression has important function during cell cycle progression and cell growth in certain cancer cells and is also involved in the development and differentiation of dental tissues. However, the function of CASP7 in breast cancer cells is unclear. The aim of this study was to analyze the expression of CASP7 in breast carcinoma patients and determine the role of CASP7 in regulating tumorigenicity in breast cancer cells. In this study, we show that the CASP7 expression is high in breast carcinoma tissues compared with normal counterpart. The ectopic expression of CASP7 is significantly associated with ERα expression status and persistently elevated in different stages of the breast tumor grades. High level of CASP7 expression showed better prognosis in breast cancer patients with systemic endocrine therapy as observed from Kaplan–Meier analysis. S3 and S4, estrogen responsive element (ERE) in the CASP7 promoter, is important for estrogen-ERα-mediated CASP7 overexpression. Increased recruitment of p300, acetylated H3 and pol II in the ERE region of CASP7 promoter is observed after hormone stimulation. Ectopic expression of CASP7 in breast cancer cells results in cell growth and proliferation inhibition via p21Cip reduction, whereas small interfering RNA (siRNA) mediated reduction of CASP7 rescued p21Cip levels. We also show that pro- and active forms of CASP7 is located in the nucleus apart from cytoplasmic region of breast cancer cells. The proliferation and growth of breast cancer cells is significantly reduced by broad-spectrum peptide inhibitors and siRNA of CASP7. Taken together, our findings show that CASP7 is aberrantly expressed in breast cancer and contributes to cell growth and proliferation by downregulating p21Cip protein, suggesting that targeting CASP7-positive breast cancer could be one of the potential therapeutic strategies. PMID:27089142

  6. Are Cellulosome Scaffolding Protein CipC and CBM3-Containing Protein HycP, Involved in Adherence of Clostridium cellulolyticum to Cellulose?

    PubMed Central

    Ferdinand, Pierre-Henri; Borne, Romain; Trotter, Valentine; Pagès, Sandrine; Tardif, Chantal; Fierobe, Henri-Pierre; Perret, Stéphanie

    2013-01-01

    Clostridium cellulolyticum, a mesophilic anaerobic bacterium, produces highly active enzymatic complexes called cellulosomes. This strain was already shown to bind to cellulose, however the molecular mechanism(s) involved is not known. In this context we focused on the gene named hycP, encoding a 250-kDa protein of unknown function, containing a Family-3 Carbohydrate Binding Module (CBM3) along with 23 hyaline repeat modules (HYR modules). In the microbial kingdom the gene hycP is only found in C. cellulolyticum and the very close strain recently sequenced Clostridium sp BNL1100. Its presence in C. cellulolyticum guided us to analyze its function and its putative role in adhesion of the cells to cellulose. The CBM3 of HycP was shown to bind to crystalline cellulose and was assigned to the CBM3b subfamily. No hydrolytic activity on cellulose was found with a mini-protein displaying representative domains of HycP. A C. cellulolyticum inactivated hycP mutant strain was constructed, and we found that HycP is neither involved in binding of the cells to cellulose nor that the protein has an obvious role in cell growth on cellulose. We also characterized the role of the cellulosome scaffolding protein CipC in adhesion of C. cellulolyticum to cellulose, since cellulosome scaffolding protein has been proposed to mediate binding of other cellulolytic bacteria to cellulose. A second mutant was constructed, where cipC was inactivated. We unexpectedly found that CipC is only partly involved in binding of C. cellulolyticum to cellulose. Other mechanisms for cellulose adhesion may therefore exist in C. cellulolyticum. In addition, no cellulosomal protuberances were observed at the cellular surface of C. cellulolyticum, what is in contrast to reports from several other cellulosomes producing strains. These findings may suggest that C. cellulolyticum has no dedicated molecular mechanism to aggregate the cellulosomes at the cellular surface. PMID:23935995

  7. Inconsistencies in tape read and tape write programs on the I-100 image analysis system

    NASA Technical Reports Server (NTRS)

    Hocutt, W. T. (Principal Investigator)

    1978-01-01

    The author has identified the following significant results. The tape read and write programs currently available on the 1-100 perform their intended functions of reading and writing tapes, but are difficult to use because they contain a number of inconsistencies. These inconsistencies can often be overcome by the use of work-around procedures and by trial and error, which is an inefficient use of expensive computer systems that should not be necessary.

  8. The Spaceborne Imaging Radar program: SIR-C - The next step toward EOS

    NASA Astrophysics Data System (ADS)

    Evans, Diane; Elachi, Charles; Cimino, Jobea

    1987-10-01

    The NASA Shuttle Imaging Radar SIR-C experiments will investigate earth surface and environment phenomena to deepen understanding of terra firma, biosphere, hydrosphere, cryosphere, and atmosphere components of the earth system, capitalizing on the observational capabilities of orbiting multiparameter radar sensors alone or in combination with other sensors. The SIR-C sensor encompasses an antenna array, an exciter, receivers, a data-handling network, and the ground SAR processor. It will be possible to steer the antenna beam electronically, so that the radar look angle can be varied.

  9. A computer program for the localization of small areas in roentgenological images

    NASA Technical Reports Server (NTRS)

    Keller, R. A.; Baily, N. A.

    1976-01-01

    A method and associated algorithm are presented which allow a simple and accurate determination to be made of the location of small symmetric areas presented in roentgenological images. The method utilizes an operator to visually spot object positions but eliminates the need for critical positioning accuracy on the operator's part. The rapidity of measurement allows results to be evaluated on-line. Parameters associated with the algorithm have been analyzed, and methods to facilitate an optimum choice for any particular experimental setup are presented.

  10. Science program for an imaging radar receiving station in Alaska. Report of the science working group

    NASA Technical Reports Server (NTRS)

    1983-01-01

    It is argued that there would be broad scientific benefit in establishing in Alaska an imaging radar receiving station that would collect data from the European Space Agency's Remote Sensing Satellite, ERS-1. This station would acquire imagery of the ice cover from the American territorial waters of the Beaufort, Chukchi, and Bering Seas. This station, in conjunction with similar stations proposed for Kiruna, Sweden, and Prince Albert, Canada would provide synoptic coverage of nearly the entire Arctic. The value of such coverage to aspects of oceanography, geology, glaciology, and botany is considered.

  11. The Spaceborne Imaging Radar program: SIR-C - The next step toward EOS

    NASA Technical Reports Server (NTRS)

    Evans, Diane; Elachi, Charles; Cimino, Jobea

    1987-01-01

    The NASA Shuttle Imaging Radar SIR-C experiments will investigate earth surface and environment phenomena to deepen understanding of terra firma, biosphere, hydrosphere, cryosphere, and atmosphere components of the earth system, capitalizing on the observational capabilities of orbiting multiparameter radar sensors alone or in combination with other sensors. The SIR-C sensor encompasses an antenna array, an exciter, receivers, a data-handling network, and the ground SAR processor. It will be possible to steer the antenna beam electronically, so that the radar look angle can be varied.

  12. Fracture toughness of CIP-HIP (cold isostatic pressed - hot isostatic pressed) beryllium at elevated temperatures. Final report, 13 May 1980-13 February 1981

    SciTech Connect

    Barker, L.M.; Jones, A.H.

    1986-04-01

    The fracture toughness of CIP-HIP (cold isostatic pressed-hot isostatic pressed) beryllium was determined using the short-bar fracture-toughness (K/sub IcSB/) method. The K/sub IcSB/ value measured was 10.96 MPa x the square root of m at room temperature. This falls well within the expected range of 9 to 12 MPa x the square root of m as observed from previous fracture toughness measurements of beryllium. Toughness increased rapidly between 400 F and 500 F reaching a value of 16.7 MPa x the square root of m at 500 F.

  13. Isolation and characterization of microsatellite loci in Coccoloba cereifera (Polygonaceae), an endangered species endemic to the Serra do Cipó, Brazil.

    PubMed

    Moreira, Rennan G; McCauley, Ross A; Cortés-Palomec, Aurea C; Lovato, M Bernadete; Fernandes, G Wilson; Oyama, Ken

    2008-07-01

    Microsatellite primers were isolated from the microendemic and threatened species Coccoloba cereifera, a shrub known only from a small region in the Serra do Cipó, Brazil. Thirteen primer pairs amplifying perfect and imperfect microsatellite regions were tested in 40 individuals from the one known occurrence of the species. Number of alleles ranged from two to six and levels of observed heterozygosities ranged from 0.21 to 0.95. These markers will be useful for the analysis of questions concerning population genetic structure and will assist in providing information for future conservation management programmes. PMID:21585911

  14. Neutron radiography activity in the european program cost 524: Neutron imaging techniques

    NASA Astrophysics Data System (ADS)

    Chirco, P.; Bach, P.; Lehmann, E.; Balasko, M.

    2001-07-01

    COST is a framework for scientific and technical cooperation, allowing the coordination of national research on a European level, including 32 member countries. Participation of institutes from non-COST countries is possible. From an initial 7 Actions in 1971, COST has grown to 200 Actions at the beginning of 2000. COST Action 524 is under materials domain, the title of which being "Neutron Imaging Techniques for the Detection of Defects in Materials", under the Chairmanship of Dr. P. Chirco (I.N.F.N.). The following countries are represented in the Management Committee of Action 524: Italy, France, Austria, Germany, United Kingdom, Hungary, Switzerland, Spain, Czech Republic, Slovenia, and Russia. The six working groups of this Action are working respectively on standardization of neutron radiography techniques, on aerospace application, on civil engineering applications, on comparison and integration of neutron imaging techniques with other NDT, on neutron tomography, and on non radiographic techniques such as neutron scattering techniques. A specific effort is devoted to standardization issues, with respect to other non European standards. Results of work performed in the COST frame are published or will be published in the review INSIGHT, edited by the British Institute of Non Destructive Testing.

  15. A description of a system of programs for mathematically processing on unified series (YeS) computers photographic images of the Earth taken from spacecraft

    NASA Technical Reports Server (NTRS)

    Zolotukhin, V. G.; Kolosov, B. I.; Usikov, D. A.; Borisenko, V. I.; Mosin, S. T.; Gorokhov, V. N.

    1980-01-01

    A description of a batch of programs for the YeS-1040 computer combined into an automated system for processing photo (and video) images of the Earth's surface, taken from spacecraft, is presented. Individual programs with the detailed discussion of the algorithmic and programmatic facilities needed by the user are presented. The basic principles for assembling the system, and the control programs are included. The exchange format within whose framework the cataloging of any programs recommended for the system of processing will be activated in the future is displayed.

  16. A comprehensive software system for image processing and programming. Final report

    SciTech Connect

    Rasure, J.; Hallett, S.; Jordan, R.

    1994-12-31

    XVision is an example of a comprehensive software system dedicated to the processing of multidimensional scientific data. Because it is comprehensive it is necessarily complex. This design complexity is dealt with by considering XVision as nine overlapping software systems, their components and the required standards. The complexity seen by a user of XVision is minimized by the different interfaces providing access to the image processing routines as well as an interface to ease the incorporation of new routines. The XVision project has stressed the importance of having: (1) interfaces to accommodate users with differing preferences and backgrounds and (2) tools to support the programmer and the scientist. The result is a system that provides a framework for building a powerful research, education and development tool.

  17. Upgrading and testing program for narrow band high resolution planetary IR imaging spectrometer

    NASA Technical Reports Server (NTRS)

    Wattson, R. B.; Rappaport, S.

    1977-01-01

    An imaging spectrometer, intended primarily for observations of the outer planets, which utilizes an acoustically tuned optical filter (ATOF) and a charge coupled device (CCD) television camera was modified to improve spatial resolution and sensitivity. The upgraded instrument was a spatial resolving power of approximately 1 arc second, as defined by an f/7 beam at the CCD position and it has this resolution over the 50 arc second field of view. Less vignetting occurs and sensitivity is four times greater. The spectral resolution of 15 A over the wavelength interval 6500 A - 11,000 A is unchanged. Mechanical utility has been increased by the use of a honeycomb optical table, mechanically rigid yet adjustable optical component mounts, and a camera focus translation stage. The upgraded instrument was used to observe Venus and Saturn.

  18. Is BodyThink an efficacious body image and self-esteem program? A controlled evaluation with adolescents.

    PubMed

    Richardson, Shanel M; Paxton, Susan J; Thomson, Julie S

    2009-03-01

    This study aimed to evaluate the efficacy of BodyThink, a widely disseminated body image and self-esteem program. Participants were 277, grade 7 students from 4 secondary schools in Australia. The intervention group (62 girls, 85 boys) participated in BodyThink during four 50-min lessons, while the control group (65 girls, 65 boys) received their usual classes. All participants completed baseline, postintervention and 3-month follow-up questionnaires. For girls, the intervention group reported higher media literacy and lower internalization of the thin ideal compared to the control group. For boys, the intervention group reported higher media literacy and body satisfaction than the control group. Although some positive outcomes were observed, it would be valuable to find ways to enhance the impact of BodyThink, especially in light of its wide dissemination. Suggestions for improving BodyThink are presented. PMID:19188101

  19. Glucose-Induced Oxidative Stress Reduces Proliferation in Embryonic Stem Cells via FOXO3A/β-Catenin-Dependent Transcription of p21(cip1).

    PubMed

    McClelland Descalzo, Darcie L; Satoorian, Tiffany S; Walker, Lauren M; Sparks, Nicole R L; Pulyanina, Polina Y; Zur Nieden, Nicole I

    2016-07-12

    Embryonic stem cells (ESCs), which are derived from a peri-implantation embryo, are routinely cultured in medium containing diabetic glucose (Glc) concentrations. While pregnancy in women with pre-existing diabetes may result in small embryos, whether such high Glc levels affect ESC growth remains uncovered. We show here that long-term exposure of ESCs to diabetic Glc inhibits their proliferation, thereby mimicking in vivo findings. Molecularly, Glc exposure increased oxidative stress and activated Forkhead box O3a (FOXO3a), promoting increased expression and activity of the ROS-removal enzymes superoxide dismutase and catalase and the cell-cycle inhibitors p21(cip1) and p27(kip1). Diabetic Glc also promoted β-catenin nuclear localization and the formation of a complex with FOXO3a that localized to the promoters of Sod2, p21(cip1), and potentially p27(kip1). Our results demonstrate an adaptive response to increases in oxidative stress induced by diabetic Glc conditions that promote ROS removal, but also result in a decrease in proliferation. PMID:27411103

  20. Phlebotomine sand fly fauna and leishmania infection in the vicinity of the Serra do Cipó National Park, a natural Brazilian heritage site.

    PubMed

    Lana, Rosana Silva; Michalsky, Érika Monteiro; Fortes-Dias, Consuelo Latorre; França-Silva, João Carlos; Lara-Silva, Fabiana de Oliveira; Lima, Ana Cristina Vianna Mariano da Rocha; Moreira de Avelar, Daniel; Martins, Juliana Cristina Dias; Dias, Edelberto Santos

    2015-01-01

    In the New World, the leishmaniases are primarily transmitted to humans through the bites of Leishmania-infected Lutzomyia (Diptera: Psychodidae) phlebotomine sand flies. Any or both of two basic clinical forms of these diseases are endemic to several cities in Brazil--the American cutaneous leishmaniasis (ACL) and the American visceral leishmaniasis (AVL). The present study was conducted in the urban area of a small-sized Brazilian municipality (Jaboticatubas), in which three cases of AVL and nine of ACL have been reported in the last five years. Jaboticatubas is an important tourism hub, as it includes a major part of the Serra do Cipó National Park. Currently, no local data is available on the entomological fauna or circulating Leishmania. During the one-year period of this study, we captured 3,104 phlebotomine sand flies belonging to sixteen Lutzomyia species. In addition to identifying incriminated or suspected vectors of ACL with DNA of the etiological agent of AVL and vice versa, we also detected Leishmania DNA in unexpected Lutzomyia species. The expressive presence of vectors and natural Leishmania infection indicates favorable conditions for the spreading of leishmaniases in the vicinity of the Serra do Cipó National Park. PMID:25793193

  1. Zac1, an Sp1-like protein, regulates human p21{sup WAF1/Cip1} gene expression in HeLa cells

    SciTech Connect

    Liu, Pei-Yao; Hsieh, Tsai-Yuan; Liu, Shu-Ting; Chang, Yung-Lung; Lin, Wei-Shiang; Wang, Wei-Ming; Huang, Shih-Ming

    2011-12-10

    Zac1 functions as both a transcription factor and a transcriptional cofactor for p53, nuclear receptors (NRs) and NR coactivators. Zac1 might also act as a transcriptional repressor via the recruitment of histone deacetylase 1 (HDAC1). The ability of Zac1 to interact directly with GC-specific elements indicates that Zac1 possibly binds to Sp1-responsive elements. In the present study, our data show that Zac1 is able to interact directly with the Sp1-responsive element in the p21{sup WAF1/Cip1} gene promoter and enhance the transactivation activity of Sp1 through direct physical interaction. Our data further demonstrate that Zac1 might enhance Sp1-specific promoter activity by interacting with the Sp1-responsive element, affecting the transactivation activity of Sp1 via a protein-protein interaction, or competing the HDAC1 protein away from the pre-existing Sp1/HDAC1 complex. Finally, the synergistic regulation of p21{sup WAF1/Cip1} gene expression by Zac1 and Sp1 is mediated by endogenous p53 protein and p53-responsive elements in HeLa cells. Our work suggests that Zac1 might serve as an Sp1-like protein that directly interacts with the Sp1-responsive element to oligomerize with and/or to coactivate Sp1.

  2. Phlebotomine Sand Fly Fauna and Leishmania Infection in the Vicinity of the Serra do Cipó National Park, a Natural Brazilian Heritage Site

    PubMed Central

    Lana, Rosana Silva; Michalsky, Érika Monteiro; Fortes-Dias, Consuelo Latorre; França-Silva, João Carlos; Lara-Silva, Fabiana de Oliveira; Lima, Ana Cristina Vianna Mariano da Rocha; Moreira de Avelar, Daniel; Martins, Juliana Cristina Dias; Dias, Edelberto Santos

    2015-01-01

    In the New World, the leishmaniases are primarily transmitted to humans through the bites of Leishmania-infected Lutzomyia (Diptera: Psychodidae) phlebotomine sand flies. Any or both of two basic clinical forms of these diseases are endemic to several cities in Brazil—the American cutaneous leishmaniasis (ACL) and the American visceral leishmaniasis (AVL). The present study was conducted in the urban area of a small-sized Brazilian municipality (Jaboticatubas), in which three cases of AVL and nine of ACL have been reported in the last five years. Jaboticatubas is an important tourism hub, as it includes a major part of the Serra do Cipó National Park. Currently, no local data is available on the entomological fauna or circulating Leishmania. During the one-year period of this study, we captured 3,104 phlebotomine sand flies belonging to sixteen Lutzomyia species. In addition to identifying incriminated or suspected vectors of ACL with DNA of the etiological agent of AVL and vice versa, we also detected Leishmania DNA in unexpected Lutzomyia species. The expressive presence of vectors and natural Leishmania infection indicates favorable conditions for the spreading of leishmaniases in the vicinity of the Serra do Cipó National Park. PMID:25793193

  3. Vibrio cholerae biofilm growth program and architecture revealed by single-cell live imaging.

    PubMed

    Yan, Jing; Sharo, Andrew G; Stone, Howard A; Wingreen, Ned S; Bassler, Bonnie L

    2016-09-01

    Biofilms are surface-associated bacterial communities that are crucial in nature and during infection. Despite extensive work to identify biofilm components and to discover how they are regulated, little is known about biofilm structure at the level of individual cells. Here, we use state-of-the-art microscopy techniques to enable live single-cell resolution imaging of a Vibrio cholerae biofilm as it develops from one single founder cell to a mature biofilm of 10,000 cells, and to discover the forces underpinning the architectural evolution. Mutagenesis, matrix labeling, and simulations demonstrate that surface adhesion-mediated compression causes V. cholerae biofilms to transition from a 2D branched morphology to a dense, ordered 3D cluster. We discover that directional proliferation of rod-shaped bacteria plays a dominant role in shaping the biofilm architecture in V. cholerae biofilms, and this growth pattern is controlled by a single gene, rbmA Competition analyses reveal that the dense growth mode has the advantage of providing the biofilm with superior mechanical properties. Our single-cell technology can broadly link genes to biofilm fine structure and provides a route to assessing cell-to-cell heterogeneity in response to external stimuli. PMID:27555592

  4. PathPics: an image-based instructional program used in the pathology and histology curriculum and transmitted over a wide area network.

    PubMed Central

    Hoffman, H. M.; Irwin, A. E.; Ligon, R. G.

    1992-01-01

    PathPics is an image review and tutorial program developed at the University of California, San Diego (UCSD) School of Medicine as an adjunct to the preclinical Pathology and Histology curriculum. It incorporates faculty expertise and provides a framework for self-paced study of this visually-oriented material. The program is served over our wide area network and runs on color-capable Macintosh computers. PathPics was added to the curriculum in January, 1992, and has been enthusiastically received by the students. Images Figure 1 PMID:1482986

  5. Analysis of Endoscopic Electronic Image of Intramucosal Gastric Carcinoma Using a Software Program for Calculating Hemoglobin Index

    PubMed Central

    Kim, Gwang Ha; Kim, Kwang Baek; Lim, Eun Kyung; Choi, Seong Ho; Kim, Tae Oh; Heo, Jeong; Kang, Dae Hwan; Cho, Mong; Park, Do Youn

    2006-01-01

    Hemoglobin is the predominent pigment in the gastrointestinal mucosa, and the development of electronic endoscopy has made it possible to quantitatively measure the mucosal hemoglobin volume, by using a hemoglobin index (IHb). The aims of this study were to make a software program to calculate the IHb and then to investigate whether the mucosal IHb determined from the electronic endoscopic data is a useful marker for evaluating the color of intramucosal gastric carcinoma with regard to its value for discriminating between the histologic types. We made a software program for calculating the IHb in the endoscopic images. By using this program, the mean values of the IHb for the carcinoma (IHb-C) and those of the IHb for the surrounding non-cancerous mucosa (IHb-N) were calculated in 75 intestinal-type and 34 diffuse-type intramucosal gastric carcinomas. We then analyzed the ratio of the IHb-C to the IHb-N (C/N ratio). The C/N ratio in the intestinal-type carcinoma group was higher than that in the diffuse-type carcinoma group (p<0.001). In the diffuse-type carcinoma group, the C/N ratio in the body was lower than that in the antrum (p=0.022). The accuracy rate, sensitivity, specificity, and the positive and negative predictive values for the differential diagnosis of the diffuse-type carcinoma from the intestinal-type carcinoma were 94.5%, 94.1%, 94.7%, 88.9% and 97.3%, respectively. IHb is useful for making quantitative measurement of the endoscopic color in the intramucosal gastric carcinoma, and the C/N ratio by using the IHb would be helpful for distinguishing the diffuse-type carcinoma from the intestinal-type carcinoma. PMID:17179684

  6. xdamp Version 6 : an IDL-based data and image manipulation program.

    SciTech Connect

    Ballard, William Parker

    2012-04-01

    The original DAMP (DAta Manipulation Program) was written by Mark Hedemann of Sandia National Laboratories and used the CA-DISSPLA{trademark} (available from Computer Associates International, Inc., Garden City, NY) graphics package as its engine. It was used to plot, modify, and otherwise manipulate the one-dimensional data waveforms (data vs. time) from a wide variety of accelerators. With the waning of CA-DISSPLA and the increasing popularity of Unix(reg sign)-based workstations, a replacement was needed. This package uses the IDL(reg sign) software, available from Research Systems Incorporated, a Xerox company, in Boulder, Colorado, as the engine, and creates a set of widgets to manipulate the data in a manner similar to the original DAMP and earlier versions of xdamp. IDL is currently supported on a wide variety of Unix platforms such as IBM(reg sign) workstations, Hewlett Packard workstations, SUN(reg sign) workstations, Microsoft(reg sign) Windows{trademark} computers, Macintosh(reg sign) computers and Digital Equipment Corporation VMS(reg sign) and Alpha(reg sign) systems. Thus, xdamp is portable across many platforms. We have verified operation, albeit with some minor IDL bugs, on personal computers using Windows 7 and Windows Vista; Unix platforms; and Macintosh computers. Version 6 is an update that uses the IDL Virtual Machine to resolve the need for licensing IDL.

  7. Research with Large Area Imaging X-Ray Telescope Sounding Rocket Program

    NASA Technical Reports Server (NTRS)

    Gorenstein, Paul

    1999-01-01

    We are engaged in a program to develop focussing hard X-ray telescopes in a double conical or Wolter 1 geometry that function up to 100 keV by employing small graze angles and multilayer coatings. Directly polished substrates are not an option because they are too thick to be nested efficiently. The only alternative is to fabricate the very thin substrates by replication. Our objective is the production of integral cylindrical substrates because they should result in better angular resolution than segmented foil geometries. In addition, integral cylinders would be more resistant to possible stress from deep multilayer coatings than segmented ones. Both electroforming of nickel (method of SkX, JET-X, and XMM) and epoxy replication are under consideration. Both processes can utilize the same types of mandrels and separation agents- While electroforming can produce substrates that are thin, the high density of the nickel may result in high weight optics for some missions. For convenience, experimentation with replication and coating is being carried out initially on flats. Our replication studies include trials with gold and carbon separation agents. This paper reports on our efforts with epoxy replicated optics.

  8. Ultra-fast digital tomosynthesis reconstruction using general-purpose GPU programming for image-guided radiation therapy.

    PubMed

    Park, Justin C; Park, Sung Ho; Kim, Jin Sung; Han, Youngyih; Cho, Min Kook; Kim, Ho Kyung; Liu, Zhaowei; Jiang, Steve B; Song, Bongyong; Song, William Y

    2011-08-01

    The purpose of this work is to demonstrate an ultra-fast reconstruction technique for digital tomosynthesis (DTS) imaging based on the algorithm proposed by Feldkamp, Davis, and Kress (FDK) using standard general-purpose graphics processing unit (GPGPU) programming interface. To this end, the FDK-based DTS algorithm was programmed "in-house" with C language with utilization of 1) GPU and 2) central processing unit (CPU) cards. The GPU card consisted of 480 processing cores (2 x 240 dual chip) with 1,242 MHz processing clock speed and 1,792 MB memory space. In terms of CPU hardware, we used 2.68 GHz clock speed, 12.0 GB DDR3 RAM, on a 64-bit OS. The performance of proposed algorithm was tested on twenty-five patient cases (5 lung, 5 liver, 10 prostate, and 5 head-and-neck) scanned either with a full-fan or half-fan mode on our cone-beam computed tomography (CBCT) system. For the full-fan scans, the projections from 157.5°-202.5° (45°-scan) were used to reconstruct coronal DTS slices, whereas for the half-fan scans, the projections from both 157.5°-202.5° and 337.5°-22.5° (2 x 45°-scan) were used to reconstruct larger FOV coronal DTS slices. For this study, we chose 45°-scan angle that contained ~80 projections for the full-fan and ~160 projections with 2 x 45°-scan angle for the half-fan mode, each with 1024 x 768 pixels with 32-bit precision. Absolute pixel value differences, profiles, and contrast-to-noise ratio (CNR) calculations were performed to compare and evaluate the images reconstructed using GPU- and CPU-based implementations. The time dependence on the reconstruction volume was also tested with (512 x 512) x 16, 32, 64, 128, and 256 slices. In the end, the GPU-based implementation achieved, at most, 1.3 and 2.5 seconds to complete full reconstruction of 512 x 512 x 256 volume, for the full-fan and half-fan modes, respectively. In turn, this meant that our implementation can process > 13 projections-per-second (pps) and > 18 pps for the full

  9. Magnetic Resonance Imaging of Changes in Abdominal Compartments in Obese Diabetics during a Low-Calorie Weight-Loss Program

    PubMed Central

    Vogt, Lena J.; Steveling, Antje; Meffert, Peter J.; Kromrey, Marie-Luise; Kessler, Rebecca; Hosten, Norbert; Krüger, Janine; Gärtner, Simone; Aghdassi, Ali A.; Mayerle, Julia; Lerch, Markus M.; Kühn, Jens-Peter

    2016-01-01

    Objectives To investigate changes in the fat content of abdominal compartments and muscle area during weight loss using confounder-adjusted chemical-shift-encoded magnetic resonance imaging (MRI) in overweight diabetics. Methods Twenty-nine obese diabetics (10/19 men/women, median age: 59.0 years, median body mass index (BMI): 34.0 kg/m2) prospectively joined a standardized 15-week weight-loss program (six weeks of formula diet exclusively, followed by reintroduction of regular food with gradually increasing energy content over nine weeks) over 15 weeks. All subjects underwent a standardized MRI protocol including a confounder-adjusted chemical-shift-encoded MR sequence with water/fat separation before the program as well at the end of the six weeks of formula diet and at the end of the program at 15 weeks. Fat fractions of abdominal organs and vertebral bone marrow as well as volumes of visceral and subcutaneous fat were determined. Furthermore, muscle area was evaluated using the L4/L5 method. Data were compared using the Wilcoxon signed-rank test for paired samples. Results Median BMI decreased significantly from 34.0 kg/m2 to 29.9 kg/m2 (p < 0.001) at 15 weeks. Liver fat content was normalized (14.2% to 4.1%, p < 0.001) and vertebral bone marrow fat (57.5% to 53.6%, p = 0.018) decreased significantly throughout the program, while fat content of pancreas (9.0%), spleen (0.0%), and psoas muscle (0.0%) did not (p > 0.15). Visceral fat volume (3.2 L to 1.6 L, p < 0.001) and subcutaneous fat diameter (3.0 cm to 2.2 cm, p < 0.001) also decreased significantly. Muscle area declined by 6.8% from 243.9 cm2 to 226.8 cm2. Conclusion MRI allows noninvasive monitoring of changes in abdominal compartments during weight loss. In overweight diabetics, weight loss leads to fat reduction in abdominal compartments, such as visceral fat, as well as liver fat and vertebral bone marrow fat while pancreas fat remains unchanged. PMID:27110719

  10. MCTP system model based on linear programming optimization of apertures obtained from sequencing patient image data maps

    SciTech Connect

    Ureba, A.; Salguero, F. J.; Barbeiro, A. R.; Jimenez-Ortega, E.; Baeza, J. A.; Leal, A.; Miras, H.; Linares, R.; Perucha, M.

    2014-08-15

    Purpose: The authors present a hybrid direct multileaf collimator (MLC) aperture optimization model exclusively based on sequencing of patient imaging data to be implemented on a Monte Carlo treatment planning system (MC-TPS) to allow the explicit radiation transport simulation of advanced radiotherapy treatments with optimal results in efficient times for clinical practice. Methods: The planning system (called CARMEN) is a full MC-TPS, controlled through aMATLAB interface, which is based on the sequencing of a novel map, called “biophysical” map, which is generated from enhanced image data of patients to achieve a set of segments actually deliverable. In order to reduce the required computation time, the conventional fluence map has been replaced by the biophysical map which is sequenced to provide direct apertures that will later be weighted by means of an optimization algorithm based on linear programming. A ray-casting algorithm throughout the patient CT assembles information about the found structures, the mass thickness crossed, as well as PET values. Data are recorded to generate a biophysical map for each gantry angle. These maps are the input files for a home-made sequencer developed to take into account the interactions of photons and electrons with the MLC. For each linac (Axesse of Elekta and Primus of Siemens) and energy beam studied (6, 9, 12, 15 MeV and 6 MV), phase space files were simulated with the EGSnrc/BEAMnrc code. The dose calculation in patient was carried out with the BEAMDOSE code. This code is a modified version of EGSnrc/DOSXYZnrc able to calculate the beamlet dose in order to combine them with different weights during the optimization process. Results: Three complex radiotherapy treatments were selected to check the reliability of CARMEN in situations where the MC calculation can offer an added value: A head-and-neck case (Case I) with three targets delineated on PET/CT images and a demanding dose-escalation; a partial breast

  11. Special Sensor Ultraviolet Limb Imager: an ionospheric and neutral density profiler for the Defense Meteorological Satellite Program satellites

    NASA Astrophysics Data System (ADS)

    McCoy, Robert P.; Dymond, Kenneth F.; Fritz, Gilbert G.; Thonnard, Stefan E.; Meier, Robert R.; Regeon, Paul A.

    1994-02-01

    The Naval Research Laboratory is developing a series of far- and extreme-ultraviolet spectrographs (800 to 1700 angstroms) to measure altitude profiles of the ionospheric and thermospheric airglow from the U.S. Air Force Defense Meteorological Satellite Program's Block 5D3 satellites. These spectrographs, **** the Special Sensor Ultraviolet Limb Imager (SSULI), use a near-Wadsworth optical configuration with a mechanical grid collimator, concave grating, and linear array detector. To image the limb, SSULI employs a rotating planar SiC mirror that sweeps the field of view perpendicular to the limb of the Earth. In the primary operating mode, the mirror sweeps the instrument field of view through 17 deg to view tangent heights from about 50 to 750 km. The SSULI detectors use microchannel plate intensification and wedge-and-strip decoding anodes to resolve 256 pixels in wavelength dispersion. The detector is windowless and uses an o-ring sealed door to protect the CsI photocathode from exposure prior to insertion in orbit. The measured altitude distributions of the airglow measured by the SSULI sensors will be used to infer the altitude distributions of electrons and neutral species. At night, electron densities will be determined by measurement of ion recombination nightglow. Daytime electron densities will be obtained from measurements of multiple resonant scattering of O+ 834-angstrom radiation produced primarily by photoionization excitation of atomic oxygen. Dayside neutral densities and temperatures will be inferred from the measurement of dayglow emissions from N2 and O produced by photoelectron impact excitation.

  12. Managing Algorithmic Skeleton Nesting Requirements in Realistic Image Processing Applications: The Case of the SKiPPER-II Parallel Programming Environment's Operating Model

    NASA Astrophysics Data System (ADS)

    Coudarcher, Rémi; Duculty, Florent; Serot, Jocelyn; Jurie, Frédéric; Derutin, Jean-Pierre; Dhome, Michel

    2005-12-01

    SKiPPER is a SKeleton-based Parallel Programming EnviRonment being developed since 1996 and running at LASMEA Laboratory, the Blaise-Pascal University, France. The main goal of the project was to demonstrate the applicability of skeleton-based parallel programming techniques to the fast prototyping of reactive vision applications. This paper deals with the special features embedded in the latest version of the project: algorithmic skeleton nesting capabilities and a fully dynamic operating model. Throughout the case study of a complete and realistic image processing application, in which we have pointed out the requirement for skeleton nesting, we are presenting the operating model of this feature. The work described here is one of the few reported experiments showing the application of skeleton nesting facilities for the parallelisation of a realistic application, especially in the area of image processing. The image processing application we have chosen is a 3D face-tracking algorithm from appearance.

  13. GPSCAN.VI: A general-purpose LabVIEW program for scanning imaging or any application requiring synchronous analog voltage generation and data acquisition

    NASA Astrophysics Data System (ADS)

    Ferrand, Patrick

    2015-07-01

    A large number of measurement techniques involve the scanning of a probe, while a physical quantity is measured. This is for instance the case for all scanning imaging methods. Data acquisition therefore requires high acquisition rates, together with an accurate synchronization between the probe control and the measurement. The GPSCAN.VI program is a general purpose LabVIEW program for the control of National Instruments high-speed data acquisition boards, allowing to design scanning imaging systems. Analog output voltages are used to drive a two-dimensional scanning device, while acquisition of analog voltages and TTL pulse counting are run in parallel. Acquisition of megapixel images with pixel dwell times down to the microsecond can be reached with negligible I/O transfer time. The design can be generalized to any situation requiring high speed synchronous generation and acquisition.

  14. Towards better digital pathology workflows: programming libraries for high-speed sharpness assessment of Whole Slide Images

    PubMed Central

    2014-01-01

    Background Since microscopic slides can now be automatically digitized and integrated in the clinical workflow, quality assessment of Whole Slide Images (WSI) has become a crucial issue. We present a no-reference quality assessment method that has been thoroughly tested since 2010 and is under implementation in multiple sites, both public university-hospitals and private entities. It is part of the FlexMIm R&D project which aims to improve the global workflow of digital pathology. For these uses, we have developed two programming libraries, in Java and Python, which can be integrated in various types of WSI acquisition systems, viewers and image analysis tools. Methods Development and testing have been carried out on a MacBook Pro i7 and on a bi-Xeon 2.7GHz server. Libraries implementing the blur assessment method have been developed in Java, Python, PHP5 and MySQL5. For web applications, JavaScript, Ajax, JSON and Sockets were also used, as well as the Google Maps API. Aperio SVS files were converted into the Google Maps format using VIPS and Openslide libraries. Results We designed the Java library as a Service Provider Interface (SPI), extendable by third parties. Analysis is computed in real-time (3 billion pixels per minute). Tests were made on 5000 single images, 200 NDPI WSI, 100 Aperio SVS WSI converted to the Google Maps format. Conclusions Applications based on our method and libraries can be used upstream, as calibration and quality control tool for the WSI acquisition systems, or as tools to reacquire tiles while the WSI is being scanned. They can also be used downstream to reacquire the complete slides that are below the quality threshold for surgical pathology analysis. WSI may also be displayed in a smarter way by sending and displaying the regions of highest quality before other regions. Such quality assessment scores could be integrated as WSI's metadata shared in clinical, research or teaching contexts, for a more efficient medical informatics

  15. Transcriptional inhibition of p21{sup WAF1/CIP1} gene (CDKN1) expression by survivin is at least partially p53-dependent: Evidence for survivin acting as a transcription factor or co-factor

    SciTech Connect

    Tang, Lei; Ling, Xiang; Liu, Wensheng; Das, Gokul M.; Li, Fengzhi

    2012-05-04

    Highlights: Black-Right-Pointing-Pointer Survivin inhibits the expression of p21 protein, mRNA and promoter activity. Black-Right-Pointing-Pointer Survivin neutralizes p53-induced p21 expression and promoter activity. Black-Right-Pointing-Pointer Survivin physically interacts with p53 in cancer cells. Black-Right-Pointing-Pointer Genetic silencing of endogenous survivin upregulates p21 in p53 wild type cancer cells. Black-Right-Pointing-Pointer Both p53 and survivin interacts on the two p53-binding sites in the p21 promoter. -- Abstract: Growing evidence suggests a role for the antiapoptotic protein survivin in promotion of cancer cell G1/S transition and proliferation. However, the underlying mechanism is unclear. Further, although upregulation of p21{sup WAF1/CIP1} by p53 plays an important role in p53-mediated cell G1 arrests in response to various distresses, it is unknown whether survivin plays a role in the regulation of p21{sup WAF1/CIP1} expression. Here, we report that exogenous expression of survivin in p53-wild type MCF-7 breast cancer cells inhibits the expression of p21{sup WAF1/CIP1} protein, mRNA and promoter activity, while the survivin C84A mutant and antisense failed to do so. Cotransfection experiments in the p53 mutant H1650 lung cancer cell line showed that survivin neutralizes p53-induced p21{sup WAF1/CIP1} expression and promoter activity. Importantly, genetically silencing of endogenous survivin using lentiviral survivin shRNA also enhances endogenous p21 in p53 wild type cancer cells, suggesting the physiological relevance of the fining. We further demonstrated that both p53 and survivin interacts on the two p53-binding sites in the p21{sup WAF1/CIP1} promoter (-2313 to -2212; -1452 to -1310), and survivin physically interacts with p53 in cancer cells. Together, we propose that survivin may act as a transcription factor or cofactor to interact with p53 on the p21{sup WAF1/CIP1} promoter leading to the inhibition of p21{sup WAF1/CIP1

  16. Hepatitis C Virus Core Protein Down-Regulates p21Waf1/Cip1 and Inhibits Curcumin-Induced Apoptosis through MicroRNA-345 Targeting in Human Hepatoma Cells

    PubMed Central

    Shiu, Tzu-Yue; Huang, Shih-Ming; Shih, Yu-Lueng; Chu, Heng-Cheng; Chang, Wei-Kuo; Hsieh, Tsai-Yuan

    2013-01-01

    Background Hepatitis C virus (HCV) has been reported to regulate cellular microRNAs. The HCV core protein is considered to be a potential oncoprotein in HCV-related hepatocellular carcinoma, but HCV core-modulated cellular microRNAs are unknown. The HCV core protein regulates p21Waf1/Cip1 expression. However, the mechanism of HCV core-associated p21Waf1/Cip1 regulation remains to be further clarified. Therefore, we attempted to determine whether HCV core-modulated cellular microRNAs play an important role in regulating p21Waf1/Cip1 expression in human hepatoma cells. Methods Cellular microRNA profiling was investigated in core-overexpressing hepatoma cells using TaqMan low density array. Array data were further confirmed by TaqMan real-time qPCR for single microRNA in core-overexpressing and full-length HCV replicon-expressing cells. The target gene of microRNA was examined by reporter assay. The gene expression was determined by real-time qPCR and Western blotting. Apoptosis was examined by annexin V-FITC apoptosis assay. Cell cycle analysis was performed by propidium iodide staining. Cell proliferation was analyzed by MTT assay. Results HCV core protein up- or down-regulated some cellular microRNAs in Huh7 cells. HCV core-induced microRNA-345 suppressed p21Waf1/Cip1 gene expression through targeting its 3′ untranslated region in human hepatoma cells. Moreover, the core protein inhibited curcumin-induced apoptosis through p21Waf1/Cip1-targeting microRNA-345 in Huh7 cells. Conclusion and Significance HCV core protein enhances the expression of microRNA-345 which then down-regulates p21Waf1/Cip1 expression. It is the first time that HCV core protein has ever been shown to suppress p21Waf1/Cip1 gene expression through miR-345 targeting. PMID:23577194

  17. Proposal for a Simple and Efficient Monthly Quality Management Program Assessing the Consistency of Robotic Image-Guided Small Animal Radiation Systems.

    PubMed

    Brodin, N Patrik; Guha, Chandan; Tomé, Wolfgang A

    2015-11-01

    Modern pre-clinical radiation therapy (RT) research requires high precision and accurate dosimetry to facilitate the translation of research findings into clinical practice. Several systems are available that provide precise delivery and on-board imaging capabilities, highlighting the need for a quality management program (QMP) to ensure consistent and accurate radiation dose delivery. An ongoing, simple, and efficient QMP for image-guided robotic small animal irradiators used in pre-clinical RT research is described. Protocols were developed and implemented to assess the dose output constancy (based on the AAPM TG-61 protocol), cone-beam computed tomography (CBCT) image quality and object representation accuracy (using a custom-designed imaging phantom), CBCT-guided target localization accuracy and consistency of the CBCT-based dose calculation. To facilitate an efficient read-out and limit the user dependence of the QMP data analysis, a semi-automatic image analysis and data representation program was developed using the technical computing software MATLAB. The results of the first 6-mo experience using the suggested QMP for a Small Animal Radiation Research Platform (SARRP) are presented, with data collected on a bi-monthly basis. The dosimetric output constancy was established to be within ±1 %, the consistency of the image resolution was within ±0.2 mm, the accuracy of CBCT-guided target localization was within ±0.5 mm, and dose calculation consistency was within ±2 s (±3%) per treatment beam. Based on these results, this simple quality assurance program allows for the detection of inconsistencies in dosimetric or imaging parameters that are beyond the acceptable variability for a reliable and accurate pre-clinical RT system, on a monthly or bi-monthly basis. PMID:26425981

  18. Assessing the Diet, Exercise, Body Image, and Weight of Adolescents: A Guide for Out-of-School Time Program Practitioners. Research-to-Results Brief. Publication #2007-06

    ERIC Educational Resources Information Center

    Martin, Laurie; Milot, Alyssa

    2007-01-01

    This brief discusses diet, exercise, body image, and weight and also provides information for practitioners on how to measure these factors among youth in their program. It summarizes (1) what it means to be overweight; (2) what are body image and eating disorders; (3) what to do if you suspect that someone in your program is suffering from an…

  19. Caffeic Acid Phenethyl Ester Causes p21Cip1 Induction, Akt Signaling Reduction, and Growth Inhibition in PC-3 Human Prostate Cancer Cells

    PubMed Central

    Lin, Hui-Ping; Jiang, Shih Sheng; Chuu, Chih-Pin

    2012-01-01

    Caffeic acid phenethyl ester (CAPE) treatment suppressed proliferation, colony formation, and cell cycle progression in PC-3 human prostate cancer cells. CAPE decreased protein expression of cyclin D1, cyclin E, SKP2, c-Myc, Akt1, Akt2, Akt3, total Akt, mTOR, Bcl-2, Rb, as well as phosphorylation of Rb, ERK1/2, Akt, mTOR, GSK3α, GSK3β, PDK1; but increased protein expression of KLF6 and p21Cip1. Microarray analysis indicated that pathways involved in cellular movement, cell death, proliferation, and cell cycle were affected by CAPE. Co-treatment of CAPE with chemotherapeutic drugs vinblastine, paclitaxol, and estramustine indicated synergistic suppression effect. CAPE administration may serve as a potential adjuvant therapy for prostate cancer. PMID:22347457

  20. DACH1 regulates cell cycle progression of myeloid cells through the control of cyclin D, Cdk 4/6 and p21{sup Cip1}

    SciTech Connect

    Lee, Jae-Woong; Kim, Hyeng-Soo; Kim, Seonggon; Hwang, Junmo; Kim, Young Hun; Lim, Ga Young; Sohn, Wern-Joo; Yoon, Suk-Ran; Kim, Jae-Young; Park, Tae Sung; Park, Kwon Moo; Ryoo, Zae Young; Lee, Sanggyu

    2012-03-30

    Highlights: Black-Right-Pointing-Pointer DACH1 increases cyclin D, F and Cdk 1, 4, 6 in mouse myeloid progenitor cells. Black-Right-Pointing-Pointer The knockdown of DACH1 blocked the cell cycle progression of HL-60 cells. Black-Right-Pointing-Pointer The novel effect of DACH1 related with cell cycle regulation and leukemogenesis. -- Abstract: The cell-fate determination factor Dachshund, a component of the Retinal Determination Gene Network (RDGN), has a role in breast tumor proliferation through the repression of cyclin D1 and several key regulators of embryonic stem cell function, such as Nanog and Sox2. However, little is known about the role of DACH1 in a myeloid lineage as a cell cycle regulator. Here, we identified the differential expression levels of extensive cell cycle regulators controlled by DACH1 in myeloid progenitor cells. The forced expression of DACH1 induced p27{sup Kip1} and repressed p21{sup Cip1}, which is a pivotal characteristic of the myeloid progenitor. Furthermore, DACH1 significantly increased the expression of cyclin D1, D3, F, and Cdk 1, 4, and 6 in myeloid progenitor cells. The knockdown of DACH1 blocked the cell cycle progression of HL-60 promyeloblastic cells through the decrease of cyclin D1, D3, F, and Cdk 1, 4, and 6 and increase in p21{sup Cip1}, which in turn decreased the phosphorylation of the Rb protein. The expression of Sox2, Oct4, and Klf4 was significantly up-regulated by the forced expression of DACH1 in mouse myeloid progenitor cells.

  1. Apoptosis, cell proliferation and modulation of cyclin-dependent kinase inhibitor p21(cip1) in vascular remodelling during vein arterialization in the rat.

    PubMed

    Borin, Thaiz Ferraz; Miyakawa, Ayumi Aurea; Cardoso, Leandro; de Figueiredo Borges, Luciano; Gonçalves, Giovana Aparecida; Krieger, Jose Eduardo

    2009-06-01

    Neo-intima development and atherosclerosis limit long-term vein graft use for revascularization of ischaemic tissues. Using a rat model, which is technically less challenging than smaller rodents, we provide evidence that the temporal morphological, cellular, and key molecular events during vein arterialization resemble the human vein graft adaptation. Right jugular vein was surgically connected to carotid artery and observed up to 90 days. Morphometry demonstrated gradual thickening of the medial layer and important formation of neo-intima with deposition of smooth muscle cells (SMC) in the subendothelial layer from day 7 onwards. Transmission electron microscopy showed that SMCs switch from the contractile to synthetic phenotype on day 3 and new elastic lamellae formation occurs from day 7 onwards. Apoptosis markedly increased on day 1, while alpha-actin immunostaining for SMC almost disappeared by day 3. On day 7, cell proliferation reached the highest level and cellular density gradually increased until day 90. The relative magnitude of cellular changes was higher in the intima vs. the media layer (100 vs. 2 times respectively). Cyclin-dependent kinase inhibitors (CDKIs) p27(Kip1) and p16(INKA) remained unchanged, whereas p21(Cip1) was gradually downregulated, reaching the lowest levels by day 7 until day 90. Taken together, these data indicate for the first time that p21(Cip1) is the main CDKI protein modulated during the arterialization process the rat model of vein arterialization that may be useful to identify and validate new targets and interventions to improve the long-term patency of vein grafts. PMID:19563615

  2. Medical imaging

    SciTech Connect

    Chapman, D.

    1996-09-01

    There are a number of medically related imaging programs at synchrotron facilities around the world. The most advanced of these are the dual energy transvenous coronary angiography imaging programs, which have progressed to human imaging for some years. The NSLS facility will be discussed and patient images from recent sessions from the NSLS and HASYLAB will be presented. The effort at the Photon Factory and Accumulator Ring will also be briefly covered, as well as future plans for the new facilities. Emphasis will be on the new aspects of these imaging programs; this includes imaging with a peripheral venous injection of the iodine contrast agent, imaging at three photon energies, and the potential of a hospital-based compact source. Other medical programs to be discussed, are the multiple energy computed tomography (MECT) project at the NSLS and plans for a MECT program at the ESRF. Recently, experiments performed at the NSLS to image mammography phantoms using monochromatic beam have produced very promising results. This program will be discussed as well as some new results from imaging a phantom using a thin Laue crystal analyzer after the object to eliminate scatter onto the detector. {copyright} {ital 1996 American Institute of Physics.}

  3. High Frequency Active Auroral Research Program (HAARP) imager. Final report, 29 August 1991-29 August 1993

    SciTech Connect

    Lance, C.; Eather, R.

    1993-09-30

    A low-light-level monochromatic imaging system was designed and fabricated which was optimized to detect and record optical emissions associated with high-power rf heating of the ionosphere. The instrument is capable of detecting very low intensities, of the order of 1 Rayleigh, from typical ionospheric atomic and molecular emissions. This is achieved through co-adding of ON images during heater pulses and subtraction of OFF (background) images between pulses. Images can be displayed and analyzed in real time and stored in optical disc for later analysis. Full image processing software is provided which was customized for this application and uses menu or mouse user interaction.

  4. An easy to use ArcMap based texture analysis program for extraction of flooded areas from TerraSAR-X satellite image

    NASA Astrophysics Data System (ADS)

    Pradhan, Biswajeet; Hagemann, Ulrike; Shafapour Tehrany, Mahyat; Prechtel, Nikolas

    2014-02-01

    Extraction of the flooded areas from synthetic aperture radar (SAR) and especially TerraSAR-X data is one of the most challenging tasks in the flood management and planning. SAR data due to its high spatial resolution and its capability of all weather conditions makes a proper choice for tropical countries. Texture is considered as an effective factor in distinguishing the classes especially in SAR imagery which records the backscatters that carry information of kind, direction, heterogeneity and relationship of the features. This paper put forward a computer program for texture analysis for high resolution radar data. Texture analysis program is introduced and discussed using the gray-level co-occurrence matrix (GLCM). To demonstrate the ability and correctness of this program, a test subset of TerraSAR-X imagery from Terengganu area, Malaysia was analyzed and pixel-based and object-based classification were attempted. The thematic maps derived by pixel-based method could not achieve acceptable visual interpretation and for that reason no accuracy assessment was performed on them. The overall accuracy achieved by object-based method was 83.63% with kappa coefficient of 0.8. Results on image texture classification showed that the proposed program is capable for texture analysis in TerraSAR-X image and the obtained textural analysis resulted in high classification accuracy. The proposed texture analysis program can be used in many applications such as land use/cover (LULC) mapping, hazard studies and many other applications.

  5. Imaging surveillance programs for women at high breast cancer risk in Europe: Are women from ethnic minority groups adequately included? (Review).

    PubMed

    Belkić, Karen; Cohen, Miri; Wilczek, Brigitte; Andersson, Sonia; Berman, Anne H; Márquez, Marcela; Vukojević, Vladana; Mints, Miriam

    2015-09-01

    Women from ethnic minority groups, including immigrants and refugees are reported to have low breast cancer (BC) screening rates. Active, culturally-sensitive outreach is vital for increasing participation of these women in BC screening programs. Women at high BC risk and who belong to an ethnic minority group are of special concern. Such women could benefit from ongoing trials aimed at optimizing screening strategies for early BC detection among those at increased BC risk. Considering the marked disparities in BC survival in Europe and its enormous and dynamic ethnic diversity, these issues are extremely timely for Europe. We systematically reviewed the literature concerning European surveillance studies that had imaging in the protocol and that targeted women at high BC risk. The aim of the present review was thereby to assess the likelihood that women at high BC risk from minority ethnic groups were adequately included in these surveillance programs. Twenty-seven research groups in Europe reported on their imaging surveillance programs for women at increased BC risk. The benefit of strategies such as inclusion of magnetic resonance imaging and/or more intensive screening was clearly documented for the participating women at increased BC risk. However, none of the reports indicated that sufficient outreach was performed to ensure that women at increased BC risk from minority ethnic groups were adequately included in these surveillance programs. On the basis of this systematic review, we conclude that the specific screening needs of ethnic minority women at increased BC risk have not yet been met in Europe. Active, culturally-sensitive outreach is needed to identify minority women at increased BC risk and to facilitate their inclusion in on-going surveillance programs. It is anticipated that these efforts would be most effective if coordinated with the development of European-wide, population-based approaches to BC screening. PMID:26134040

  6. Derivative Quotient Spectrophotometry and an Eco-Friendly Micellar Chromatographic Approach with Time-Programmed UV-Detection for the Separation of Two Fluoroquinolones and Phenazopyridine.

    PubMed

    Tolba, Manar M; Salim, Mohamed M

    2016-05-01

    In this study, two analytical approaches were exploited for the resolution of binary mixtures of ciprofloxacin HCl (CIP) or norfloxacin (NOR) and phenazopyridine HCl (PHZ). In the first approach, the amplitudes of the first derivative of the ratio spectra were measured at 267 or 287 nm for CIP and at 268 or 291 nm for NOR. PHZ could be directly determined in the presence of CIP or NOR at 405 nm. The calibration graphs were rectilinear over the ranges of 1.0-16.0 µg/mL for CIP or NOR and 1.0-10.0 µg/mL for PHZ. In the second approach, an accurate, reliable and environmentally nontoxic micellar liquid chromatographic (MLC) method was developed. A good chromatographic separation was achieved using a 150 mm × 4.6 mm i.d., 5 µm particle size Spherisorb ODS-2 column. Eco-friendly mobile phase containing 0.12 M sodium dodecyl sulphate, 0.3% triethylamine and 6%n-butanol in 0.02 M orthophosphoric acid of pH 3.0 was pumped at a flow rate of 1 mL/min. Time programmed UV-detection was applied to allow sensitive determination of the studied drugs. The analytes were eluted without interferences in <10 min. Methocarbamol was used as an internal standard. The MLC method was found to be rectilinear over the concentration range of 0.5-20.0 μg/mL for CIP, NOR or PHZ. These optimized and validated methods were successfully applied for the simultaneous analysis of the studied drugs in their synthetic mixtures and co-formulated tablets. Moreover, the second method was further extended to the determination of these drugs in human urine with direct injection and without any pretreatment. PMID:26867555

  7. Body Imaging

    NASA Technical Reports Server (NTRS)

    1990-01-01

    Magnetic Resonance Imaging (MRI) and Computer-aided Tomography (CT) images are often complementary. In most cases, MRI is good for viewing soft tissue but not bone, while CT images are good for bone but not always good for soft tissue discrimination. Physicians and engineers in the Department of Radiology at the University of Michigan Hospitals are developing a technique for combining the best features of MRI and CT scans to increase the accuracy of discriminating one type of body tissue from another. One of their research tools is a computer program called HICAP. The program can be used to distinguish between healthy and diseased tissue in body images.

  8. Resveratrol and curcumin synergistically induces apoptosis in cigarette smoke condensate transformed breast epithelial cells through a p21(Waf1/Cip1) mediated inhibition of Hh-Gli signaling.

    PubMed

    Mohapatra, Purusottam; Satapathy, Shakti Ranjan; Siddharth, Sumit; Das, Dipon; Nayak, Anmada; Kundu, Chanakya Nath

    2015-09-01

    Combination therapy using two or more small molecule inhibitors of aberrant signaling cascade in aggressive breast cancers is a promising therapeutic strategy over traditional monotherapeutic approaches. Here, we have studied the synergistic mechanism of resveratrol and curcumin induced apoptosis using in vitro (cigarette smoke condensate mediated transformed breast epithelial cell, MCF-10A-Tr) and in vivo (tumor xenograft mice) model system. Resveratrol exposure increased the intracellular uptake of curcumin in a dose dependent manner and caused apoptosis in MCF-10A-Tr cells. Approximately, ten fold lower IC50 value was noted in cells treated with the combination of resveratrol (3μM) and curcumin (3μM) in comparison to 30μM of resveratrol or curcumin alone. Resveratrol+curcumin combination caused apoptosis by increasing Bax/Bcl-xL ratio, Cytochrome C release, cleaved product of PARP and caspase 3 in cells. Interestingly, this combination unaltered the protein expressions of WNT-TCF and Notch signaling components, β-catenin and cleaved notch-1 val1744, respectively. Furthermore, the combination also significantly decreased the intermediates of Hedgehog-Gli cascade including SMO, SHH, Gli-1, c-MYC, Cyclin-D1, etc. and increased the level of p21(Waf/Cip1) in vitro and in vivo. A significant reduction of Gli- promoter activity was noted in combinational drug treated cells in comparison to individual drug treatment. Un-alteration of the expressions of the above proteins and Gli1 promoter activity in p21(Waf/Cip1) knockout cells suggests this combination caused apoptosis through p21(Waf/Cip1). Thus, our findings revealed resveratrol and curcumin synergistically caused apoptosis in cigarette smoke induced breast cancer cells through p2(Waf/Cip1) mediated inhibition of Hedgehog-Gli cascade. PMID:26212257

  9. The role of KLF4 in UVB-induced murine skin tumor development and its correlation with cyclin D1, p53, and p21(Waf1/Cip1) in epithelial tumors of the human skin.

    PubMed

    Choi, Woo Jin; Youn, Sung Hwan; Back, Jung Ho; Park, Saebomi; Park, Eun Joo; Kim, Kwang Joong; Park, Hye Rim; Kim, Arianna L; Kim, Kwang Ho

    2011-04-01

    The zinc-finger-type transcriptional factor KLF4 is expressed in a variety of tissues including skin. KLF4 can function as either a tumor suppressor or an oncogene, depending on the type of tissue in which it is expressed, by modulating the expression of various factors. To understand the role of KLF4 in human skin cancer and also to evaluate the expression of cyclin D1, p53, and p21(Waf1/Cip1) in relation to the expression of KLF4, we evaluated the pattern of KLF4 expression during UVB-induced skin tumor development in SKH-1 hairless mice and in human skin cancer. We also determined whether there are correlations between the expression of KLF4, cyclin D1, p53, and p21 and non-melanoma skin tumors. KLF4 expression was found in the basal layer of non-irradiated control murine skin. Chronic UVB irradiation caused a progressive decrease in KLF4 expression, which was substantially decreased in UVB-induced murine skin tumors. In human precancerous lesions, KLF4 expression was maintained in 64.3% of Bowen's disease samples and 90.0% of AK samples. In contrast, KLF4 expression was significantly reduced in human cancer lesions (p = 0.004). A positive correlation was found between the expression of KLF4 and p21(Waf1/Cip1) in AK, whereas there was a negative correlation between the expression of cyclin D1 and p21(Waf1/Cip1) in Bowen's disease. Thus, our results suggest that KLF4 may function as a tumor suppressor in the skin and that the deregulated expression of KLF4 in the context of p21(Waf1/Cip1) and cyclin D1 expression may be involved in skin tumorigenesis. PMID:21132436

  10. Down-regulation of dihydrofolate reductase inhibits the growth of endothelial EA.hy926 cell through induction of G1 cell cycle arrest via up-regulating p53 and p21waf1/cip1 expression

    PubMed Central

    Fei, Zhewei; Gao, Yong; Qiu, Mingke; Qi, Xianqin; Dai, Yuxin; Wang, Shuqing; Quan, Zhiwei; Liu, Yingbin; Ou, Jingmin

    2016-01-01

    Folic acid supplementation may meliorate cardiovascular disease risk by improving vascular endothelial structure and function. However, the underlying mechanisms are still lack of a global understanding. To be used, folic acid must be converted to 7,8-dihydrofolate by dihydrofolate reductase to generate one-carbon derivatives serving as important cellular cofactors in the synthesis of nucleotides and amino acids required for cell growth. Therefore, this study explored the effect of dihydrofolate reductase knockdown on endothelial EA.hy926 cell growth and the mechanism involved. We found that down-regulation of dihydrofolate reductase inhibited EA.hy926 cell proliferation, and induced G1 phase arrest. Meanwhile, the expression of regulators necessary for G1/S phase transition, such as cyclin-dependent kinases CDK2, CDK4 and CDK6, were remarkably down-regulated; by contrast, the cell cycle inhibitors p21waf/cip1, p27Kip1 and p53 were significantly up-regulated after dihydrofolate reductase knockdown. Furthermore, supplementation of 5-methyltetrahydrofolate to the dihydrofolate reductase knockdown cells could weaken the inhibitory effect of dihydrofolate reductase knockdown on cell proliferation, simultaneously, inducing the expression of p53 and p21waf/cip1 falling back moderately. Our findings suggest that attenuating dihydrofolate reductase may cause imbalanced expression of cell cycle regulators, especially up-regulation of p53-p21waf/cip1 pathway, leading to G1 cell cycle arrest, thereby inhibiting the growth of endothelial EA.hy926 cells. PMID:27013776

  11. Improving Literacy Skills and Self-Image within the Daycare Teacher Training Program through an Emphasis on Cultural Heritage.

    ERIC Educational Resources Information Center

    Buchan, Barbara

    A consultant and guest trainer implemented a practicum intervention designed to enable the graduation of 10 at-risk Latina women from a day care teacher training program. The trainees had been identified as being at-risk of dropping out of the program due to a lack of English-as-a-Second-Language (ESL) and literacy skills, low self-esteem, and…

  12. Is an "ideal" service institution image the same for all referral sources? The case of chemical dependency treatment programs.

    PubMed

    Johnson, K; LaTour, M S

    1993-01-01

    In a competitive market like chemical dependency treatment, segmenting the professional referral market according to an "ideal" service image may offer a service institution a strategic advantage. Results of this study suggest that while different professionals in a referral market may attach differential importance to the same service feature, a favorable or unfavorable "image" seems to encompass how well both the professional and the professionals' client are treated by the service institution. PMID:10129241

  13. The US Geological Survey's side-looking airborne radar acquisition program: Image data from the Rocky Mountains to the Pacific

    SciTech Connect

    Kovar, A.N.; Schoonmaker, J.W. Jr. )

    1993-04-01

    The US Geological Survey (USGS) has been systematically collecting side-looking airborne radar (SLAR) image data for the US since 1980. The image strip swaths, ranging in width from 20 to 46 km, are acquired commercially by X-band (3 cm) radar systems. Data are acquired with 60 percent side-lap for better mosaic preparation and stereoscopic capability. The image strips are assembled into 1[degree] x 2[degree] mosaic quadrangles that are based on the USGS 1:250,000-topographic map series for control, format, and nomenclature. These mosaics present the data in a broad synoptic view that facilitates geologic interpretation. SLAR image mosaics have been prepared for more than 35 percent of the US west of the Rocky Mountain front. In addition to quadrangle mosaics, regional composite mosaics have been prepared as value-added products. These include Pacific Northwest (14 quadrangles), southern California Coastal (from San Francisco to San Diego), Reno-Walker (includes parts of Yellowstone and Grand Teton National Parks), Uinta Basin (Salt Lake City, Price and Grand Junction), and Salton Sea Region (San Diego, Santa Ana, El Centro and Salton Sea). Most of the image data are available on computer compatible tapes and photographic products. To make the data more accessible and reasonably priced, the strip images are being processed into CD-ROM (compact disc, read-only memory). One demonstration CD-ROM includes the mosaics of Las Vegas, Mariposa, Ritzville, Walla Walla, and Pendleton quadrangles.

  14. Translational Upregulation of an Individual p21Cip1 Transcript Variant by GCN2 Regulates Cell Proliferation and Survival under Nutrient Stress.

    PubMed

    Lehman, Stacey L; Cerniglia, George J; Johannes, Gregg J; Ye, Jiangbin; Ryeom, Sandra; Koumenis, Constantinos

    2015-06-01

    Multiple transcripts encode for the cell cycle inhibitor p21(Cip1). These transcripts produce identical proteins but differ in their 5' untranslated regions (UTRs). Although several stresses that induce p21 have been characterized, the mechanisms regulating the individual transcript variants and their functional significance are unknown. Here we demonstrate through (35)S labeling, luciferase reporter assays, and polysome transcript profiling that activation of the Integrated Stress Response (ISR) kinase GCN2 selectively upregulates the translation of a p21 transcript variant containing 5' upstream open reading frames (uORFs) through phosphorylation of the eukaryotic translation initiation factor eIF2α. Mutational analysis reveals that the uORFs suppress translation under basal conditions, but promote translation under stress. Functionally, ablation of p21 ameliorates G1/S arrest and reduces cell survival in response to GCN2 activation. These findings uncover a novel mechanism of p21 post-transcriptional regulation, offer functional significance for the existence of multiple p21 transcripts, and support a key role for GCN2 in regulating the cell cycle under stress. PMID:26102367

  15. Promoter-associated endogenous and exogenous small RNAs suppress human bladder cancer cell metastasis by activating p21 (CIP1/WAF1) expression.

    PubMed

    Wang, Chenghe; Ge, Qiangqiang; Chen, Zhong; Hu, Jia; Li, Fan; Ye, Zhangqun

    2016-05-01

    Accumulating data suggest that micro RNAs (miRNAs) or double-stranded RNAs (dsRNAs) can activate gene expression by targeting promoters. The cyclin-dependent kinase inhibitor p21 (CIP1/WAF1) (p21) has also been shown to suppress epithelial-mesenchymal transition (EMT) which plays a crucial role in the early stage of tumor metastases and invasiveness. In a previous study, we have reported that miR-370-5p is low-expressed in bladder cancer (BCa) tissues and cell lines. Here, we identified that miR-370-5p and sequence homology dsRNA (dsP21-555) fully complementary to promoter hold the potent abilities to induce p21 expression. Moreover, transfection of miR-370-5p or dsP21-555 into BCa cells remarkably inverts EMT-associated genes (increases epithelial cell makers E-cadherin and β-catenin, and decreases mesenchymal cell markers ZEB1 and Vimentin) expression mainly via regulating p21 expression. Besides, through manipulating p21, both the candidates can retard BCa cell migration and invasion. In summary, our results provide evidence that both endogenous and exogenous small RNAs may function to induce p21 expression by interacting with the similar promoter region and impede BCa metastasis. PMID:26643891

  16. Synergistic effects of dexamethasone and genistein on the expression of Cdk inhibitor p21WAF1/CIP1 in human hepatocellular and colorectal carcinoma cells.

    PubMed

    Park, J H; Oh, E J; Choi, Y H; Kang, C D; Kang, H S; Kim, D K; Kang, K I; Yoo, M A

    2001-05-01

    Previous studies have shown that dexamethasone, a synthetic glucocorticoid, can induce a G1 arrest, however, genistein, a natural isoflavonoid phytoestrogen, induces a G2/M arrest in the cell cycle progression in various cancer cell lines. A block of cell cycle checkpoint by dexamethasone and genistein correlates with a selective induction of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1 in a tumor suppressor p53-independent manner and abolishment of Cdk2 phosphorylation. In the present study, the effects of dexamethasone and genistein (both singly and combined) on the expression of p21 in human hepatocellular Hep G2 and colorectal Colo320 HSR carcinoma cells were evaluated. Whereas dexamethasone mildly induced the level of p21 protein, genistein strongly increased the expression of p21 protein in our experimental condition. Both compounds also activated p21 promoter reporter constructs. The combined effects of dexamethasone and genistein on the induction of p21 protein and activation of p21 promoter were synergistic in both cell lines. These findings indicate that dexamethasone and genistein act in a synergistic fashion and have potential for combination chemotherapy for the treatment of liver and colon cancer. PMID:11295047

  17. Translational Upregulation of an Individual p21Cip1 Transcript Variant by GCN2 Regulates Cell Proliferation and Survival under Nutrient Stress

    PubMed Central

    Lehman, Stacey L.; Cerniglia, George J.; Johannes, Gregg J.; Ye, Jiangbin; Ryeom, Sandra; Koumenis, Constantinos

    2015-01-01

    Multiple transcripts encode for the cell cycle inhibitor p21Cip1. These transcripts produce identical proteins but differ in their 5’ untranslated regions (UTRs). Although several stresses that induce p21 have been characterized, the mechanisms regulating the individual transcript variants and their functional significance are unknown. Here we demonstrate through 35S labeling, luciferase reporter assays, and polysome transcript profiling that activation of the Integrated Stress Response (ISR) kinase GCN2 selectively upregulates the translation of a p21 transcript variant containing 5’ upstream open reading frames (uORFs) through phosphorylation of the eukaryotic translation initiation factor eIF2α. Mutational analysis reveals that the uORFs suppress translation under basal conditions, but promote translation under stress. Functionally, ablation of p21 ameliorates G1/S arrest and reduces cell survival in response to GCN2 activation. These findings uncover a novel mechanism of p21 post-transcriptional regulation, offer functional significance for the existence of multiple p21 transcripts, and support a key role for GCN2 in regulating the cell cycle under stress. PMID:26102367

  18. 2010 Mississippi Curriculum Framework: Postsecondary Health-Care Assistant. (Program CIP-51.3902 - Nurse/Nursing Assistant/Aide and Client Care Assistant)

    ERIC Educational Resources Information Center

    Jackson, Jennifer; Sims, Addie

    2010-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  19. 2011 Mississippi Curriculum Framework: Postsecondary Diagnostic Medical Sonography Technology. (Program CIP: 51.0910 - Diagnostic Medical Sonography/Sonographer and Ultrasound)

    ERIC Educational Resources Information Center

    Finch, Wanda; Wilson, Lesa

    2011-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…

  20. 2007 Mississippi Curriculum Framework: Postsecondary Diagnostic Medical Sonography Technology. (Program CIP: 51.0910 - Diagnostic Medical Sonography/Sonographer and Ultrasound Technician)

    ERIC Educational Resources Information Center

    Larimore, Crystal; Megginson, Nita; Weekley, Tracy B.

    2007-01-01

    As the world economy continues to evolve, businesses and industries must adopt new practices and processes in order to survive. Quality and cost control, work teams and participatory management, and an infusion of technology are transforming the way people work and do business. Employees are now expected to read, write, and communicate…