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Sample records for institutului politehnic din

  1. Diné (Navajo) Ethno- and Archaeoastronomy

    NASA Astrophysics Data System (ADS)

    Chamberlain, Von Del

    The Navajo (Diné) are an Athabascan-speaking people who migrated from the far northwest of America into the desert southwest where they became the largest surviving Native American culture. Three words portray Diné philosophy - beauty, harmony, and balance. Their traditions are rich with astronomical symbolism found in literature, ceremony, iconography, artifacts, rock art, and the sacred landscape. This chapter summarizes Diné astronomical traditions, identification of stars known to be important to the Diné, and how these are depicted on artifacts and rock art.

  2. Chromosomal locations of three Bacillus subtilis din genes

    SciTech Connect

    Gillespie, K.; Yasbin, R.E.

    1987-07-01

    Previously isolated DNA damage-inducible (din) genes of Bacillus subtilis have been mapped on the bacterial chromosome by bacteriophage PBS1-mediated transduction. The din genes have been localized to three positions on the B. subtilis map. dinA cotransduction with the hisA locus was 80%, while dinC cotransduction with this marker was about 56%. dinB is unlinked to hisA, but its cotransduction with the dal-1 and purB loci was 84 and 22%, respectively.

  3. Strategy of DIN-PACS benchmark testing

    NASA Astrophysics Data System (ADS)

    Norton, Gary S.; Lyche, David K.; Richardson, Nancy E.; Thomas, Jerry A.; Romlein, John R.; Cawthon, Michael A.; Lawrence, David P.; Shelton, Philip D.; Parr, Laurence F.; Richardson, Ronald R., Jr.; Johnson, Steven L.

    1998-07-01

    The Digital Imaging Network -- Picture Archive and Communication System (DIN-PACS) procurement is the Department of Defense's (DoD) effort to bring military medical treatment facilities into the twenty-first century with nearly filmless digital radiology departments. The DIN-PACS procurement is unique from most of the previous PACS acquisitions in that the Request for Proposals (RFP) required extensive benchmark testing prior to contract award. The strategy for benchmark testing was a reflection of the DoD's previous PACS and teleradiology experiences. The DIN-PACS Technical Evaluation Panel (TEP) consisted of DoD and civilian radiology professionals with unique clinical and technical PACS expertise. The TEP considered nine items, key functional requirements to the DIN-PACS acquisition: (1) DICOM Conformance, (2) System Storage and Archive, (3) Workstation Performance, (4) Network Performance, (5) Radiology Information System (RIS) functionality, (6) Hospital Information System (HIS)/RIS Interface, (7) Teleradiology, (8) Quality Control, and (9) System Reliability. The development of a benchmark test to properly evaluate these key requirements would require the TEP to make technical, operational, and functional decisions that had not been part of a previous PACS acquisition. Developing test procedures and scenarios that simulated inputs from radiology modalities and outputs to soft copy workstations, film processors, and film printers would be a major undertaking. The goals of the TEP were to fairly assess each vendor's proposed system and to provide an accurate evaluation of each system's capabilities to the source selection authority, so the DoD could purchase a PACS that met the requirements in the RFP.

  4. Selection of dinB Alleles Suppressing Survival Loss upon dinB Overexpression in Escherichia coli

    PubMed Central

    Benson, Ryan W.; Cafarelli, Tiziana M.; Rands, Thomas J.; Lin, Ida

    2014-01-01

    Escherichia coli strains overproducing DinB undergo survival loss; however, the mechanisms regulating this phenotype are poorly understood. Here we report a genetic selection revealing DinB residues essential to effect this loss-of-survival phenotype. The selection uses strains carrying both an antimutator allele of DNA polymerase III (Pol III) α-subunit (dnaE915) and either chromosomal or plasmid-borne dinB alleles. We hypothesized that dnaE915 cells would respond to DinB overproduction differently from dnaE+ cells because the dnaE915 allele is known to have an altered genetic interaction with dinB+ compared to its interaction with dnaE+. Notably, we observe a loss-of-survival phenotype in dnaE915 strains with either a chromosomal catalytically inactive dinB(D103N) allele or a low-copy-number plasmid-borne dinB+ upon DNA damage treatment. Furthermore, we find that the loss-of-survival phenotype occurs independently of DNA damage treatment in a dnaE915 strain expressing the catalytically inactive dinB(D103N) allele from a low-copy-number plasmid. The selective pressure imposed resulted in suppressor mutations that eliminated growth defects. The dinB intragenic mutations examined were either base pair substitutions or those that we inferred to be loss of function (i.e., deletions and insertions). Further analyses of selected novel dinB alleles, generated by single-base-pair substitutions in the dnaE915 strain, indicated that these no longer effect loss of survival upon overproduction in dnaE+ strains. These mutations are mapped to specific areas of DinB; this permits us to gain insights into the mechanisms underlying the DinB-mediated overproduction loss-of-survival phenotype. PMID:24914188

  5. Experience with gasket testing according to new DIN standards

    SciTech Connect

    Kockelmann, H.; Bartonicek, J.; Hirschvogel, A.

    1996-12-01

    In 1995 new DIN standards were published containing gasket factor definitions, gasket testing procedures and requirements as a basis for technical delivery conditions. The gasket factors in DIN 28090 are consistent with those in the design rules for bolted flanged connections laid down in DIN EN 1591. Both standards together are an advanced and reliable tool for strength analysis and tightness assessment for bolted flanged connections. The new DIN standards contain new features in detail: gasket factors are related to tightness classes allowing tightness classification of flanged joints; time and temperature effects are regarded by means of high temperature longterm aging leakage tests; and gasket material degradation due to chemical attack is covered by means of chemical resistance tests. Verification of gasket testing according to the new DIN standards by means of tests on real flanged joints is under way.

  6. Ernst Rüdin: Hitler's Racial Hygiene Mastermind.

    PubMed

    Joseph, Jay; Wetzel, Norbert A

    2013-01-01

    Ernst Rüdin (1874-1952) was the founder of psychiatric genetics and was also a founder of the German racial hygiene movement. Throughout his long career he played a major role in promoting eugenic ideas and policies in Germany, including helping formulate the 1933 Nazi eugenic sterilization law and other governmental policies directed against the alleged carriers of genetic defects. In the 1940s Rüdin supported the killing of children and mental patients under a Nazi program euphemistically called "Euthanasia." The authors document these crimes and discuss their implications, and also present translations of two publications Rüdin co-authored in 1938 showing his strong support for Hitler and his policies. The authors also document what they see as revisionist historical accounts by leading psychiatric genetic authors. They outline three categories of contemporary psychiatric genetic accounts of Rüdin and his work: (A) those who write about German psychiatric genetics in the Nazi period, but either fail to mention Rüdin at all, or cast him in a favorable light; (B) those who acknowledge that Rüdin helped promote eugenic sterilization and/or may have worked with the Nazis, but generally paint a positive picture of Rüdin's research and fail to mention his participation in the "euthanasia" killing program; and (C) those who have written that Rüdin committed and supported unspeakable atrocities. The authors conclude by calling on the leaders of psychiatric genetics to produce a detailed and complete account of their field's history, including all of the documented crimes committed by Rüdin and his associates. PMID:23180223

  7. Role of the DinB Homologs Rv1537 and Rv3056 in Mycobacterium tuberculosis▿ †

    PubMed Central

    Kana, Bavesh D.; Abrahams, Garth L.; Sung, Nackmoon; Warner, Digby F.; Gordhan, Bhavna G.; Machowski, Edith E.; Tsenova, Liana; Sacchettini, James C.; Stoker, Neil G.; Kaplan, Gilla; Mizrahi, Valerie

    2010-01-01

    The environment encountered by Mycobacterium tuberculosis during infection is genotoxic. Most bacteria tolerate DNA damage by engaging specialized DNA polymerases that catalyze translesion synthesis (TLS) across sites of damage. M. tuberculosis possesses two putative members of the DinB class of Y-family DNA polymerases, DinB1 (Rv1537) and DinB2 (Rv3056); however, their role in damage tolerance, mutagenesis, and survival is unknown. Here, both dinB1 and dinB2 are shown to be expressed in vitro in a growth phase-dependent manner, with dinB2 levels 12- to 40-fold higher than those of dinB1. Yeast two-hybrid analyses revealed that DinB1, but not DinB2, interacts with the β-clamp, consistent with its canonical C-terminal β-binding motif. However, knockout of dinB1, dinB2, or both had no effect on the susceptibility of M. tuberculosis to compounds that form N2-dG adducts and alkylating agents. Similarly, deletion of these genes individually or in combination did not affect the rate of spontaneous mutation to rifampin resistance or the spectrum of resistance-conferring rpoB mutations and had no impact on growth or survival in human or mouse macrophages or in mice. Moreover, neither gene conferred a mutator phenotype when expressed ectopically in Mycobacterium smegmatis. The lack of the effect of altering the complements or expression levels of dinB1 and/or dinB2 under conditions predicted to be phenotypically revealing suggests that the DinB homologs from M. tuberculosis do not behave like their counterparts from other organisms. PMID:20139184

  8. Characterization of three mycobacterial DinB (DNA polymerase IV) paralogs highlights DinB2 as naturally adept at ribonucleotide incorporation

    PubMed Central

    Ordonez, Heather; Uson, Maria Loressa; Shuman, Stewart

    2014-01-01

    This study unveils Mycobacterium smegmatis DinB2 as the founder of a clade of Y-family DNA polymerase that is naturally adept at incorporating ribonucleotides by virtue of a leucine in lieu of a canonical aromatic steric gate. DinB2 efficiently scavenges limiting dNTP and rNTP substrates in the presence of manganese. DinB2's sugar selectivity factor, gauged by rates of manganese-dependent dNMP versus rNMP addition, is 2.7- to 3.8-fold. DinB2 embeds ribonucleotides during DNA synthesis when rCTP and dCTP are at equimolar concentration. DinB2 can incorporate at least 16 consecutive ribonucleotides. In magnesium, DinB2 has a 26- to 78-fold lower affinity for rNTPs than dNTPs, but only a 2.6- to 6-fold differential in rates of deoxy versus ribo addition (kpol). Two other M. smegmatis Y-family polymerases, DinB1 and DinB3, are characterized here as template-dependent DNA polymerases that discriminate strongly against ribonucleotides, a property that, in the case of DinB1, correlates with its aromatic steric gate side chain. We speculate that the unique ability of DinB2 to utilize rNTPs might allow for DNA repair with a ‘ribo patch’ when dNTPs are limiting. Phylogenetic analysis reveals DinB2-like polymerases, with leucine, isoleucine or valine steric gates, in many taxa of the phylum Actinobacteria. PMID:25200080

  9. Characterization of the DNA damage-inducible helicase DinG from Escherichia coli.

    PubMed

    Voloshin, Oleg N; Vanevski, Filip; Khil, Pavel P; Camerini-Otero, R Daniel

    2003-07-25

    The dinG promoter was first isolated in a genetic screen scoring for damage-inducible loci in Escherichia coli (Lewis, L. K., Jenkins, M. E., and Mount, D. W. (1992) J. Bacteriol. 174, 3377-3385). Sequence analysis suggests that the dinG gene encodes a putative helicase related to a group of eukaryotic helicases that includes mammalian XPD (Koonin, E. V. (1993) Nucleic Acids Res. 21, 1497), an enzyme involved in transcription-coupled nucleotide excision repair and basal transcription. We have characterized the dinG gene product from E. coli using genetic and biochemical approaches. Deletion of dinG has no severe phenotype, indicating that it is non-essential for cell viability. Both dinG deletion and over-expression of the DinG protein from a multicopy plasmid result in a slight reduction of UV resistance. DinG, purified as a fusion protein from E. coli cells, behaves as a monomer in solution, as judged from gel filtration experiments. DinG is an ATP-hydrolyzing enzyme; single-stranded (ss) DNA stimulates the ATPase activity 15-fold. Kinetic data yield a Hill coefficient of 1, consistent with one ATP-hydrolyzing site per DinG molecule. DinG possesses a DNA helicase activity; it translocates along ssDNA in a 5' --> 3' direction, as revealed in experiments with substrates containing non-natural 5'-5' and 3'-3' linkages. The ATP-dependent DNA helicase activity of DinG requires divalent cations (Mg2+, Ca2+, and Mn2+) but is not observed in the presence of Zn2+. The DinG helicase does not discriminate between ribonucleotide and deoxyribonucleotide triphosphates, and it unwinds duplex DNA with similar efficiency in the presence of ATP or dATP. We discuss the possible involvement of the DinG helicase in DNA replication and repair processes. PMID:12748189

  10. Two Modes of Binding of DinI to RecA Filament Provide a New Insight Into Regulation of SOS Response by DinI Protein

    PubMed Central

    Galkin, Vitold E.; Britt, Rachel L.; Bane, Lukas B.; Yu, Xiong; Cox, Michael M.; Egelman, Edward H.

    2011-01-01

    The RecA protein plays a principal role in the bacterial SOS response to DNA damage. The induction of the SOS response is well understood and involves the cleavage of the LexA repressor catalyzed by the RecA nucleoprotein filament. In contrast, our understanding of the regulation and termination of the SOS response is much more limited. RecX and DinI are two major regulators of RecA’s ability to promote LexA cleavage and a strand exchange reaction and are believed to modulate its activity in ongoing SOS events. DinI’s function in the SOS response remains controversial since its interaction with the RecA filament is concentration-dependent and may result in either stabilization or depolymerization of the filament. The 17 C-terminal residues of RecA modulate the interaction between DinI and RecA. We demonstrate that DinI binds to the active RecA filament in two distinct structural modes. In the first mode DinI binds to the C-terminus of a RecA protomer. In the second mode DinI resides deeply in the groove of the RecA filament with its negatively charged C-terminal helix proximal to the L2 loop of RecA. The deletion of the 17 C-terminal residues of RecA favors the second mode of binding. We suggest that the negatively charged C-terminus of RecA prevents DinI from entering the groove and protects the RecA filament from depolymerization. Polymorphic binding of DinI to RecA filaments implies an even more complex role of DinI in the bacterial SOS response. PMID:21458462

  11. Doctor Shoja-ad-Din Sheikholeslamzadeh and his Achievements

    PubMed Central

    Afshar, Ahmadreza; Ziaei, Majid Eyvaz; Ahmadi, Aziz

    2015-01-01

    Doctor Shoja-ad-Din Sheikholeslamzadeh (also known as Dr. Sheikh) (1931-2014) was an outstanding orthopedic surgeon. He is credited for the establishment of the Iranian Association of Rehabilitation, Shafa Yahyaian Hospital as an Orthopedic and Rehabilitation Center, the Social Welfare Organization, Iran's emergency dispatch center (115) and many other major projects that led to the upgrading of health care services in Iran. He also served as the Minister of Social Welfare and Minister of Health and Welfare before the Islamic revolution. The history of modern health care management and modern orthopedic surgery in Iran are indebted to the great leadership and executive abilities of Dr. Sheikh. PMID:25692161

  12. A ΔdinB mutation that sensitizes Escherichia coli to the lethal effects of UV and X-radiation

    PubMed Central

    Lee, Mei-Chong W.; Franco, Magdalena; Vargas, Doris M.; Hudman, Deborah A.; White, Steven J.; Fowler, Robert G.; Sargentini, Neil J.

    2014-01-01

    The DinB (PolIV) protein of Escherichia coli participates in several cellular functions. We investigated a dinB mutation, Δ(dinB-yafN)883(::kan) [referred to as ΔdinB883], which strongly sensitized E. coli cells to both UV- and X-radiation killing. Earlier reports indicated dinB mutations had no obvious effect on UV radiation sensitivity which we confirmed by showing that normal UV radiation sensitivity is conferred by the ΔdinB749 allele. Compared to a wild-type strain, the ΔdinB883 mutant was most sensitive (160-fold) in early to mid-logarithmic growth phase and much less sensitive (twofold) in late log or stationary phases, thus showing a growth phase-dependence for UV radiation sensitivity. This sensitizing effect of ΔdinB883 is assumed to be completely dependent upon the presence of UmuDC protein; since the ΔdinB883 mutation did not sensitize the ΔumuDC strain to UV radiation killing throughout log phase and early stationary phase growth. The DNA damage checkpoint activity of UmuDC was clearly affected by ΔdinB883 as shown by testing a umuC104 ΔdinB883 double-mutant. The sensitivities of the ΔumuDC strain and the ΔdinB883 ΔumuDC double-mutant strain were significantly greater than for the ΔdinB883 strain, suggesting that the ΔdinB883 allele only partially suppresses UmuDC activity. The ΔdinB883 mutation partially sensitized (fivefold) uvrA and uvrB strains to UV radiation, but did not sensitize a ΔrecA strain. A comparison of the DNA sequences of the ΔdinB883 allele with the sequences of the Δ(dinB-yafN)882(::kan) and ΔdinB749 alleles, which do not sensitize cells to UV radiation, revealed ΔdinB883 is likely a “gain-of-function” mutation. The ΔdinB883 allele encodes the first 54 amino acids of wild-type DinB followed by 29 predicted residues resulting from the continuation of the dinB reading frame into an adjacent insertion fragment. The resulting polypeptide is proposed to interfere directly or indirectly with UmuDC function

  13. Solution structure of DinI provides insight into its mode of RecA inactivation.

    PubMed Central

    Ramirez, B. E.; Voloshin, O. N.; Camerini-Otero, R. D.; Bax, A.

    2000-01-01

    The Escherichia coli RecA protein triggers both DNA repair and mutagenesis in a process known as the SOS response. The 81-residue E. coli protein DinI inhibits activity of RecA in vivo. The solution structure of DinI has been determined by multidimensional triple resonance NMR spectroscopy, using restraints derived from two sets of residual dipolar couplings, obtained in bicelle and phage media, supplemented with J couplings and a moderate number of NOE restraints. DinI has an alpha/beta fold comprised of a three-stranded beta-sheet and two alpha-helices. The beta-sheet topology is unusual: the central strand is flanked by a parallel and an antiparallel strand and the sheet is remarkably flat. The structure of DinI shows that six negatively charged Glu and Asp residues on DinI's kinked C-terminal alpha-helix form an extended, negatively charged ridge. We propose that this ridge mimics the electrostatic character of the DNA phospodiester backbone, thereby enabling DinI to compete with single-stranded DNA for RecA binding. Biochemical data confirm that DinI is able to displace ssDNA from RecA. PMID:11152126

  14. Ernst Rüdin, 1874-1952: a German psychiatrist and geneticist.

    PubMed

    Weber, M M

    1996-07-26

    Ernst Rüdin (1874-1952) was one of the major representatives of German psychiatry, genetics, and eugenics in the first half of the twentieth century. Born in Switzerland, he was influenced early on by his brother-in-law Alfred Ploetz, who propagated the ideas of social Darwinism and "racial hygiene" in Germany after 1890. Rüdin began his career in psychiatry at Emil Kraepelin's clinic in Munich, where he developed the concept of "empirical genetic prognosis" of mental disorders. He published his first results on the genetics of schizophrenia in 1916. From 1917-1945 Rüdin was director of the Genealogical-Demographic Department at the German Institute for Psychiatric Research, which Kraepelin had founded. After a short interruption from 1925-1928, Rüdin returned to Munich and enlarged the department. After 1933 the National Socialist government and party endorsed Rüdin's work by supplying financial and manpower support. Nazi health policy required a scientific basis to justify its actions, and Rüdin's ideas corresponded partially with this kind of thinking. In 1934 he prepared the official commentary on the "Law for the Prevention of Genetically Diseased Offspring." The connections of Rüdin's department to National Socialism can be understood as one of the main reasons for the critical attitude towards psychiatric genetics in Germany after 1945. PMID:8837697

  15. Manufacturing of microcomponents in a research institute under DIN EN ISO 9001

    NASA Astrophysics Data System (ADS)

    Maas, Dieter; Karl, Bernhard; Saile, Volker; Schulz, Joachim

    2000-08-01

    The Institute for Microstructure Technology at Forschungszentrum Karlsruhe has implemented a rigorous quality management system and was certified according to the DIN ISO EN 9001 standard in January 2000.

  16. Developing a salinity-based approach for the evaluation of DIN removal rate in estuarine ecosystems.

    PubMed

    Hong, Yiguo; Wang, Shuailong; Xu, Xiang-Rong; Wu, Jiapeng; Liu, Ling; Yue, Weizhong; Wu, Meilin; Wang, Youshao

    2015-10-01

    Estuaries play an important role in the removal of overloading nitrogen to relieve the eutrophic pressure of coastal seawater. However, the exact amount of nitrogen removed in estuarine ecosystems is difficult to be estimated because of the complex dynamic mixing process between riverine water and coastal seawater. In this study, a new method was developed to calculate the removal rate of dissolved inorganic nitrogen (DIN) in estuarine waters attributed to the mixing process and was based on the assumption that relative salinity can serve as an indicator of the degree of mixing. This assumption was supported by the experimental results that demonstrated a linear regression relationship between DIN decline and salinity increase Thus, the decreased amount of DIN in mixing waters attributed to the dilution effect could be determined with the salinity as an index. With this model, the DIN removal rate in both Chesapeake Bay and Pearl River Estuary were defined. As predicted, our analysis demonstrated that the DIN removal rate increased gradually from upstream to downstream in both studied estuaries with obvious seasonable variation pattern: high in warm seasons and low in cold seasons. The practical application of this method might be affected by multiple factors, including the geographic landform of estuaries, initial estuaries DIN concentration, the DIN concentration in seawater, DIN importing from tributaries, sewage discharge and hydrodynamic mixing. Therefore, the results supported the hypothesis that estuaries have a strong capability to remove the nitrogen inputted from human activities, especially in warm season and therefore should play an important role in regulating the balance of global nitrogen biogeochemical cycle. PMID:25957975

  17. Apportioning riverine DIN load to export coefficients of land uses in an urbanized watershed.

    PubMed

    Shih, Yu-Ting; Lee, Tsung-Yu; Huang, Jr-Chuan; Kao, Shuh-Ji; Chang

    2016-08-01

    The apportionment of riverine dissolved inorganic nitrogen (DIN) load to individual land use on a watershed scale demands the support of accurate DIN load estimation and differentiation of point and non-point sources, but both of them are rarely quantitatively determined in small montane watersheds. We introduced the Danshui River watershed of Taiwan, a mountainous urbanized watershed, to determine the export coefficients via a reverse Monte Carlo approach from riverine DIN load. The results showed that the dynamics of N fluctuation determines the load estimation method and sampling frequency. On a monthly sampling frequency basis, the average load estimation of the methods (GM, FW, and LI) outperformed that of individual method. Export coefficient analysis showed that the forest DIN yield of 521.5kg-Nkm(-2)yr(-1) was ~2.7-fold higher than the global riverine DIN yield (mainly from temperate large rivers with various land use compositions). Such a high yield was attributable to high rainfall and atmospheric N deposition. The export coefficient of agriculture was disproportionately larger than forest suggesting that a small replacement of forest to agriculture could lead to considerable change of DIN load. The analysis of differentiation between point and non-point sources showed that the untreated wastewater (non-point source), accounting for ~93% of the total human-associated wastewater, resulted in a high export coefficient of urban. The inclusion of the treated and untreated wastewater completes the N budget of wastewater. The export coefficient approach serves well to assess the riverine DIN load and to improve the understanding of N cascade. PMID:27093117

  18. Assessing sinkhole activity in the Ebro Valley mantled evaporite karst using advanced DInSAR

    NASA Astrophysics Data System (ADS)

    Galve, Jorge Pedro; Castañeda, Carmen; Gutiérrez, Francisco; Herrera, Gerardo

    2015-01-01

    Sinkholes in karst areas may cause subsidence damage in transportation infrastructures, demolition of buildings and even the loss of human lives when they occur in a catastrophic way. Differential Interferometry (DInSAR) is a promising technology for detecting and characterizing sinkholes, as well as for reducing the associated risk when combined with other sources of data such as a sinkhole inventory. In this work, the usefulness of InSAR techniques and data for sinkhole risk management has been analyzed through the comparison of three DInSAR-derived velocity maps with a comprehensive sinkhole inventory in the Ebro Valley, NE Spain. The DInSAR maps have contributed to improve the sinkhole inventory in different ways: (1) detection of non-inventoried sinkholes; (2) revision of sinkhole areas previously classified as inactive as active; and (3) refinement of underestimated sinkhole boundaries. The obtained results suggest that DInSAR products are suitable for analyzing active dissolution-induced subsidence. The application of these techniques may help in recognizing and better characterizing previously unknown karst subsidence problems and in preventing personal and property losses. However, the analysis reveals that the available DInSAR maps combined overlook about 70% of the previously mapped active sinkholes mainly due to decorrelation.

  19. Estudio dinámico de un potencial perturbador dependiente de dos parámetros

    NASA Astrophysics Data System (ADS)

    Miloni, O.; Brunini, A.

    El objeto del presente trabajo consiste en el estudio dinámico de un sistema dinámico caracterizado por la función hamiltoniana correspondiente a un satélite planetario perturbado por la acción del Sol y del achatamiento del planeta madre. Cuando dicha Hamiltoniana se promedia respecto de los términos de corto período, esta queda con dos grados de libertad, y su estudio puede ser realizado con las herramientas clásicas de la dinámica no-lineal. Se tratará de determinar regiones regulares y caóticas de movimiento. En el caso de estas últimas, es de particular interés la determinación de su orígen.

  20. The DinB Superfamily Includes Novel Mycothiol, Bacillithiol and Glutathione S-transferases

    PubMed Central

    Newton, Gerald L.; Leung, Stephan S.; Wakabayashi, Judy I.; Rawat, Mamta; Fahey, Robert C.

    2011-01-01

    The superfamily of glutathione S-transferases has been the subject of extensive study but Actinobacteria produce mycothiol (MSH) in place of glutathione and no mycothiol S-transferase (MST) has been identified. Using mycothiol and monochlorobimane as substrates a MST activity was detected in extracts of Mycobacterium smegmatis and purified sufficiently to allow identification of MSMEG_0887, a member the DUF664 family of the DinB superfamily, as the MST. The identity of the M. smegmatis and homologous Mycobacterium tuberculosis (Rv0443) enzymes was confirmed by cloning and the expressed proteins were found to be active with MSH but not bacillithiol (BSH) or glutathione (GSH). Bacillus subtilis YfiT is another member of the DinB superfamily but this bacterium produces BSH. The YfiT protein was shown to have S-transferase activity with monochlorobimane when assayed with BSH but not with MSH or GSH. Enterococcus faecalis EF_3021 shares some homology with MSMEG_0887 but this organism produces GSH but not MSH or BSH. Cloned and expressed EF_0321 was active with monochlorobimane and GSH but not with MSH or BSH. MDMPI_2 is another member of the DinB superfamily and has been previously shown to have mycothiol-dependent maleylpyruvate isomerase activity. Three of the eight families of the DinB superfamily include proteins shown to catalyze thiol-dependent metabolic or detoxification activities. Since more than two-thirds of the sequences assigned to the DinB superfamily are members of these families it seems likely that such activity is dominant in the DinB superfamily. PMID:22059487

  1. Characteristics of the DIN-2PI spectrometer with a neutron concentrator

    NASA Astrophysics Data System (ADS)

    Kalinin, I. V.; Morozov, V. M.; Novikov, A. G.; Puchkov, A. V.; Savostin, V. V.; Sudarev, V. V.; Bulkin, A. P.; Kalinin, S. I.; Pusenkov, V. M.; Ul'yanov, V. A.

    2014-02-01

    The basic characteristics of the upgraded DIN-2PI spectrometer are reported. Based on experimental data and numerical calculation results, it is concluded that a supermirror neutron concentrator, with which the DIN-2PI spectrometer is equipped, raises the density of the cold neutron flux at the sample almost by an order of magnitude. The use of a grooved moderator in channel no. 2 and upgrading of the core of the IBR-2M reactor increases the neutron flux density on the sample by about 40% more.

  2. Modeling Of Small Scale Surface Deformation Based On DInSAR Result

    NASA Astrophysics Data System (ADS)

    Furuta, Ryoichi

    2012-01-01

    Monitoring of small scale surface deformation as subsidence and landslides is very important to protect lifeline, manmade structures, buildings and houses, roads and railways, and so on. In order to monitor subsidence and landslides, several technologies were applied. However, these technologies cannot be applied to broad area and it is difficult to understand spatial distribution of affected area of subsidence and landslides. DInSAR analysis can be understand spatial distribution of affected area of subsidence and landslide, therefore, it has high prospect in Japan because Japan has a lot of sites of subsidence and landslides. However, DInSAR analysis cannot extract sub-surface information like depth of source of deformation. In order to enhance a capability of detection of small scale surface deformation as subsidence and landslide by DInSAR analysis, integration use of DInSAR analysis and numerical model and original model for estimation of small scale surface deformation was proposed in this paper. Proposed model was applied to subsidence which was detected by ALOS PALSAR DInSAR. Target area is located in the North-East part of China. At the target area, large subsidence occurred due to coal mining activity and overuse of ground water. In order to consider way of countermeasure work for subsidence, it is important to know affected area of subsidence, depth of source of subsidence, and so on. Affected area of subsidence can be understood by the DInSAR analysis, however, depth of source of subsidence has difficult to understand by the DInSAR analysis. In order to understand the depth of source of subsidence, numerical model was necessary. Proposed model can be estimating affected area of subsidence and depth of source of subsidence by a few parameters derived from DInSAR analysis. Proposed model was well performed in the simulation of subsidence pattern of 1-D space and it could be estimated depth of subsidence source. However, it wasn't well performed to

  3. A novel cis-acting element required for DNA damage-inducible expression of yeast DIN7

    SciTech Connect

    Yoshitani, Ayako; Yoshida, Minoru; Ling Feng

    2008-01-04

    Din7 is a DNA damage-inducible mitochondrial nuclease that modulates the stability of mitochondrial DNA (mtDNA) in Saccharomyces cerevisiae. How DIN7 gene expression is regulated, however, has remained largely unclear. Using promoter sequence alignment, we found a highly conserved 19-bp sequence in the promoter regions of DIN7 and NTG1, which encodes an oxidative stress-inducible base-excision-repair enzyme. Deletion of the 19-bp sequence markedly reduced the hydroxyurea (HU)-enhanced DIN7 promoter activity. In addition, nuclear fractions prepared from HU-treated cells were used in in vitro band shift assays to reveal the presence of currently unidentified trans-acting factor(s) that preferentially bound to the 19-bp region. These results suggest that the 19-bp sequence is a novel cis-acting element that is required for the regulation of DIN7 expression in response to HU-induced DNA damage.

  4. The Din in the Head, Input, and the Language Acquisition Device.

    ERIC Educational Resources Information Center

    Krashen, Stephen D.

    1983-01-01

    Involuntary mental rehearsal of foreign language words, sounds, and phrases is found consistent with current second language acquisition theory and case history reports. It is suggested the "din in the head" results from stimulation of the language acquisition device set off when the acquirer receives enough comprehensible input. (Author/MSE)

  5. Connection and Community: Diné College Emphasizes Real-World Experience in Public Health

    ERIC Educational Resources Information Center

    Bauer, Mark

    2016-01-01

    The Summer Research Enhancement Program (SREP) at Diné College provides students with a solid foundation of public health research methods and includes a hands-on internship in their home community to test their newly acquired skills while enhancing the communities' health. Focusing on health issues prioritized by Navajo health leaders, from…

  6. Assessment of DInSAR Potential in Simulating Geological Subsurface Structure

    NASA Astrophysics Data System (ADS)

    Fouladi Moghaddam, N.; Rudiger, C.; Samsonov, S. V.; Hall, M.; Walker, J. P.; Camporese, M.

    2013-12-01

    High resolution geophysical surveys, including seismic, gravity, magnetic, etc., provide valuable information about subsurface structuring but they are very costly and time consuming with non-unique and sometimes conflicting interpretations. Several recent studies have examined the application of DInSAR to estimate surface deformation, monitor possible fault reactivation and constrain reservoir dynamic behaviour in geothermal and groundwater fields. The main focus of these studies was to generate an elevation map, which represents the reservoir extraction induced deformation. This research study, however, will focus on developing methods to simulate subsurface structuring and identify hidden faults/hydraulic barriers using DInSAR surface observations, as an innovative and cost-effective reconnaissance exploration tool for planning of seismic acquisition surveys in geothermal and Carbon Capture and Sequestration regions. By direct integration of various DInSAR datasets with overlapping temporal and spatial coverage we produce multi-temporal ground deformation maps with high resolution and precision to evaluate the potential of a new multidimensional MSBAS technique (Samsonov & d'Oreye, 2012). The technique is based on the Small Baseline Subset Algorithm (SBAS) that is modified to account for variation in sensor parameters. It allows integration of data from sensors with different wave-band, azimuth and incidence angles, different spatial and temporal sampling and resolutions. These deformation maps then will be used as an input for inverse modelling to simulate strain history and shallow depth structure. To achieve the main objective of our research, i.e. developing a method for coupled InSAR and geophysical observations and better understanding of subsurface structuring, comparing DInSAR inverse modelling results with previously provided static structural model will result in iteratively modified DInSAR structural model for adequate match with in situ observations

  7. Railway deformation detected by DInSAR over active sinkholes in the Ebro Valley evaporite karst, Spain

    NASA Astrophysics Data System (ADS)

    Galve, J. P.; Castañeda, C.; Gutiérrez, F.

    2015-11-01

    Subsidence was measured for the first time on railway tracks in the central sector of Ebro Valley (NE Spain) using Differential Synthetic Aperture Radar Interferometry (DInSAR) techniques. This area is affected by evaporite karst and the analysed railway corridors traverse active sinkholes that produce deformations in these infrastructures. One of the railway tracks affected by slight settlements is the Madrid-Barcelona high-speed line, a form of transport infrastructure highly vulnerable to ground deformation processes. Our analysis based on DInSAR measurements and geomorphological surveys indicates that this line shows dissolution-induced subsidence and compaction of anthropogenic deposits (infills and embankments). Significant sinkhole-related subsidence was also measured by DInSAR techniques on the Castejón-Zaragoza conventional railway line. This study demonstrates that DInSAR velocity maps, coupled with detailed geomorphological surveys, may help in the identification of the railway track sections that are affected by active subsidence.

  8. Effects of different N sources on riverine DIN export and retention in a subtropical high-standing island, Taiwan

    NASA Astrophysics Data System (ADS)

    Huang-Chuan, Jr.; Lee, Tsung-Yu; Lin, Teng-Chiu; Hein, Thomas; Lee, Li-Chin; Shih, Yu-Ting; Kao, Shuh-Ji; Shiah, Fuh-Kwo; Lin, Neng-Huei

    2016-03-01

    Increases in nitrogen (N) availability and mobility resulting from anthropogenic activities have substantially altered the N cycle, both locally and globally. Taiwan characterized by the subtropical montane landscape with abundant rainfall, downwind of the most rapidly industrializing eastern coast of China, can be a demonstration site for extremely high N input and riverine DIN (dissolved inorganic N) export. We used 49 watersheds with similar climatic and landscape settings but classified into low, moderate, and highly disturbed categories based on population density to illustrate their differences in nitrogen inputs (through atmospheric N deposition, synthetic fertilizers, and human emission) and DIN export ratios. Our results showed that the island-wide average riverine DIN export is ˜ 3800 kg N km-2 yr-1, approximately 18 times the global average. The average riverine DIN export ratios are 0.30-0.51, which are much higher than the averages of 0.20-0.25 of large rivers around the world, indicating excessive N input relative to ecosystem demand or retention capacity. The low disturbed watersheds have a high N retention capacity and DIN export ratios of 0.06-0.18 in spite of the high N input (˜ 4900 kg N km-2 yr-1). The high retention capacity is likely due to effective uptake by secondary forests in the watersheds. The moderately disturbed watersheds show a linear increase in DIN export with increases in total N inputs and mean DIN export ratios of 0.20 to 0.31. The main difference in land use between low and moderately disturbed watersheds is the greater proportion of agricultural land cover in the moderately disturbed watersheds. Thus, their greater DIN export could be attributed to N fertilizers used in the agricultural lands. The greater export ratios also imply that agricultural lands have a lower proportional N retention capacity and that reforestation could be an effective land management practice to reduce riverine DIN export. The export ratios of the

  9. Effects of different N sources on riverine DIN export and retention in a subtropical high-standing island, Taiwan

    PubMed Central

    Huang, Jr-Chuan; Lee, Tsung-Yu; Lin, Teng-Chiu; Hein, Thomas; Lee, Li-Chin; Shih, Yu-Ting; Kao, Shuh-Ji; Shiah, Fuh-Kwo; Lin, Neng-Huei

    2016-01-01

    Increases in nitrogen (N) availability and mobility resulting from anthropogenic activities have substantially altered the N cycle, both locally and globally. Taiwan characterized by the subtropical montane landscape with abundant rainfall, downwind of the most rapidly industrializing eastern coast of China, can be a demonstration site for extremely high N input and riverine DIN (dissolved inorganic N) export. We used 49 watersheds with similar climatic and landscape settings but classified into low, moderate, and highly disturbed categories based on population density to illustrate their differences in nitrogen inputs (through atmospheric N deposition, synthetic fertilizers, and human emission) and DIN export ratios. Our results showed that the island-wide average riverine DIN export is ~ 3800 kg N km−2 yr−1, approximately 18 times the global average. The average riverine DIN export ratios are 0.30−0.51, which are much higher than the averages of 0.20−0.25 of large rivers around the world, indicating excessive N input relative to ecosystem demand or retention capacity. The low disturbed watersheds have a high N retention capacity and DIN export ratios of 0.06−0.18 in spite of the high N input (~ 4900 kg N km−2 yr−1). The high retention capacity is likely due to effective uptake by secondary forests in the watersheds. The moderately disturbed watersheds show a linear increase in DIN export with increases in total N inputs and mean DIN export ratios of 0.20 to 0.31. The main difference in land use between low and moderately disturbed watersheds is the greater proportion of agricultural land cover in the moderately disturbed watersheds. Thus, their greater DIN export could be attributed to N fertilizers used in the agricultural lands. The greater export ratios also imply that agricultural lands have a lower proportional N retention capacity and that reforestation could be an effective land management practice to reduce riverine DIN export. The export

  10. On the application of DInSAR to deformation monitoring in desert areas

    NASA Astrophysics Data System (ADS)

    Chang, Xiao-Tao; Guo, Jin-Yun; Zhang, Yong-Hong; Wang, Xiao-Qing

    2011-03-01

    The DInSAR technique is used for monitoring the desert height changes to study sandstorms. Hunshandake Sandy Land, as the test area, is one of the main sources of sandstorms in Beijing. In order to study the sandstorm source and its impact, a pair of EnviSat ASAR images of Oct. 11, 2005, and Oct. 26, 2004, is processed on the basis of analysis of six ERS-2 and EnviSat radar images. After the image configuration, flat earth effect correction, data filtering, phase unwrapping, and geo-coding, a deformation model over Hunshandake desert is built. According to the results, the height decreased in most areas and increased in a few areas, which basically coincides with the strong sandstorm appearing in Beijing in the Spring of 2005. The results show DInSAR has an important role in monitoring of desert surface deformation.

  11. Ground deformation of Tenerife volcano island revealed by 1992-2005 DInSAR time series:

    NASA Astrophysics Data System (ADS)

    Tizzani, P.

    2009-04-01

    We study the state of deformation of Tenerife Island using Differential Synthetic Aperture Radar Interferometry (DInSAR). We apply the Small BAseline Subset (SBAS) DInSAR algorithm to radar images acquired from 1992 to 2005 by ERS sensors to determine the deformation rate distribution and the time series for the coherent pixels identified in the island. Our analysis reveals that the summit area of the volcanic edifice is characterized by a continuous subsidence extending well beyond Las Cañadas caldera rim and corresponding to the intrusive core of the island. These results, coupled with GPS ones, structural and geological information and deformation modelling, suggest that the intrusive complex is subsiding into a weak lithosphere and that the volcanic edifice is in a state of compression. We also detect more localized deformation patterns correlated with water table changes and variations in the time deformation associated with the seismic crisis in 2004.

  12. The helicases DinG, Rep and UvrD cooperate to promote replication across transcription units in vivo.

    PubMed

    Boubakri, Hasna; de Septenville, Anne Langlois; Viguera, Enrique; Michel, Bénédicte

    2010-01-01

    How living cells deal with head-on collisions of the replication and transcription complexes has been debated for a long time. Even in the widely studied model bacteria Escherichia coli, the enzymes that take care of such collisions are still unknown. We report here that in vivo, the DinG, Rep and UvrD helicases are essential for efficient replication across highly transcribed regions. We show that when rRNA operons (rrn) are inverted to face replication, the viability of the dinG mutant is affected and over-expression of RNase H rescues the growth defect, showing that DinG acts in vivo to remove R-loops. In addition, DinG, Rep and UvrD exert a common function, which requires the presence of two of these three helicases. After replication blockage by an inverted rrn, Rep in conjunction with DinG or UvrD removes RNA polymerase, a task that is fulfilled in its absence by the SOS-induced DinG and UvrD helicases. Finally, Rep and UvrD also act at inverted sequences other than rrn, and promote replication through highly transcribed regions in wild-type E. coli. PMID:19851282

  13. New A-DInSAR study of La Palma, Canary Islands, Spain. Results and interpretation.

    NASA Astrophysics Data System (ADS)

    Escayo, Joaquín; Bru, Guadalupe; Camacho, Antonio; Prieto, Juan F.; Mallorquí, Jordi J.; Fernández, José

    2016-04-01

    We present new results on the deformation field for La Palma Island in the Canary Archipelago using the complete archive of Envisat (2004-2010). The recent volcanic activity registered in La Palma (at historical and subhistorical times, there have been seven eruptions, in ca. A.D. 1480, 1585, 1646, 1677, 1712, 1949 and 1971) and the absence of any geodetic monitoring system implemented in the Island at the 90's converts A-DInSAR studies in an basic information source for the study of surface displacements. From late 90's several geodetic studies using GNSS, gravimetry and InSAR techniques has been carried out in the island (see Fernández et al., 2015 for a summary). We improve previous radar satellite results by increasing the temporal coverage and using more images, which generates a greater interferogram set, and using a recently developed A-DInSAR processing technique which includes an error estimation for the LOS mean velocity and deformation time series ("Subsidence" software, developed by the "Universitat Politècnica de Catalunya, see e.g., Blanco-Sánchez et al., 2008, and Centolanza, 2015). Our A-DInSAR results are compared with previous InSAR ones and with GNSS results obtained from campaigns. A discussion and interpretation of the results is also presented. Radar images have been provided by ESA through Cat.-1 13933 project.

  14. Enhancing DInSAR capabilities for landslide monitoring by applying GIS-based multicriteria filtering analysis

    NASA Astrophysics Data System (ADS)

    Beyene, F.; Knospe, S.; Busch, W.

    2015-04-01

    Landslide detection and monitoring remain difficult with conventional differential radar interferometry (DInSAR) because most pixels of radar interferograms around landslides are affected by different error sources. These are mainly related to the nature of high radar viewing angles and related spatial distortions (such as overlays and shadows), temporal decorrelations owing to vegetation cover, and speed and direction of target sliding masses. On the other hand, GIS can be used to integrate spatial datasets obtained from many sources (including radar and non-radar sources). In this paper, a GRID data model is proposed to integrate deformation data derived from DInSAR processing with other radar origin data (coherence, layover and shadow, slope and aspect, local incidence angle) and external datasets collected from field study of landslide sites and other sources (geology, geomorphology, hydrology). After coordinate transformation and merging of data, candidate landslide representing pixels of high quality radar signals were filtered out by applying a GIS based multicriteria filtering analysis (GIS-MCFA), which excludes grid points in areas of shadow and overlay, low coherence, non-detectable and non-landslide deformations, and other possible sources of errors from the DInSAR data processing. At the end, the results obtained from GIS-MCFA have been verified by using the external datasets (existing landslide sites collected from fieldworks, geological and geomorphologic maps, rainfall data etc.).

  15. Effects of different N sources on riverine DIN export and retention in subtropical high-standing island, Taiwan

    NASA Astrophysics Data System (ADS)

    Huang, J.-C.; Lee, T.-Y.; Lin, T.-C.; Hein, T.; Lee, L.-C.; Shih, Y.-T.; Kao, S.-J.; Shiah, F.-K.; Lin, N.-H.

    2015-10-01

    Increases in nitrogen (N) availability and mobility resulting from anthropogenic activities has substantially altered N cycle both locally and globally. Taiwan characterized by the subtropical montane landscape with abundant rainfall, downwind to the most rapidly industrializing east coast of China can be a demonstration site for extreme high N input and riverine DIN (dissolved inorganic N) export. We used 49 watersheds classified into low-, moderate-, and highly-disturbed categories based on population density to illustrate their differences in nitrogen inputs through atmospheric N deposition, synthetic fertilizers and human emission and DIN export ratios. Our results showed that the island-wide average riverine DIN export is ~ 3800 kg N km-2 yr-1, approximately 18-fold higher than the global average mostly due to the large input of synthetic fertilizers. The average riverine DIN export ratio is 0.30-0.51, which is much higher than the average of 0.20-0.25 of large rivers around the world indicating excessive N input relative to ecosystem demand or retention capacity. The low-disturbed watersheds, despite of high N input, only export 0.06-0.18 of the input so were well buffered to changes in input quantity suggesting high efficiency of nitrogen usage or high N retention capacity of the less disturbed watersheds. The high retention capacity probably is due to the effective uptake by secondary forests in the watersheds. The moderate-disturbed watersheds show a linear increase of output with increases in total N inputs and a mean DIN export ratio of 0.20 to 0.31. The main difference in land use between low and moderately disturbed watershed is the relative proportions of agricultural land and forests, not the built-up lands. Thus, their greater DIN export quantity could be attributed to N fertilizers used in the agricultural lands. The greater export ratios also imply that agricultural lands have lower proportional N retention capacity and that reforestation could be

  16. EPOSAR: an innovative service to provide EPOS community with advanced DInSAR products

    NASA Astrophysics Data System (ADS)

    Manunta, Michele; De Luca, Claudio; Elefante, Stefano; Lanari, Riccardo; Pepe, Antonio; Zinno, Ivana; Casu, Francesco

    2015-04-01

    The quantitative evaluation of ground deformation is traditionally based on in-situ surveying techniques that, through the intensive use of GPS stations, automatic total stations and levelling benchmarks, can measure up to sub-centimetre displacements. In the last decades, the extensive use of satellite remote sensing data, such as Synthetic Aperture Radar (SAR) images, has represented an important breakthrough in the context of non-invasive ground deformation analyses over large areas, thanks to their large spatial coverage and relatively short revisit time, as well as to their medium-high ground resolution. In such a context, the well-known Differential SAR Interferometry (DInSAR) technique allows us to map and measure deformation phenomena due to both natural and man-made causes with centimetre to millimetre accuracy. The Earth Science community has a wide interest in the use of DInSAR displacement maps both for crisis management and risk mitigation activities, and for surveillance, monitoring and analysis of geophysical phenomena. In areas characterized by high level of hazards the availability of routinely generated advanced DInSAR products would allow a fast analysis of their current status, providing a near real time monitoring. Similarly, an on-demand service would allow the customization of the products by selecting the area of interest, the SAR data to be processed, and other processing parameters to be set by the users to edit/correct/improve the final products. In this work we discuss the Satellite Data Thematic Core Service of EPOS and we present the EPOSAR service. In particular, the EPOSAR service, based on the well-known DInSAR approach referred to as Small Baseline Subset (SBAS), accomplishes a shared and synergic Earth Observation (EO) service aimed at designing, implementing and harmonizing efficient satellite data processing chains capable of ingesting the significantly increased data stream expected from the ESA Sentinel-1 satellites. EPOSAR

  17. Evolución Dinámica de Cúmulos Estelares Abiertos

    NASA Astrophysics Data System (ADS)

    de La Fuente Marcos, R.

    Las estrellas que observamos no se formaron aisladamente, sino que lo hicieron en grupos o cúmulos. Estos sistemas estelares recibenel nombre de cúmulos estelares abiertos o galácticos cuando están constituidos por estrellas de la Población I. Los cúmulos abiertos se localizan preferentemente en el plano galáctico, y en muchos de ellos está teniendo lugar aún una activa formación estelar. El propósito principal de esta tesis doctoral es el estudio de la evolución dinámica de estos sistemas estelares por medio de simulaciones numéricas y de la comparación de sus resultados con datos observacionales. Con este fin se analizan los efectos que, sobre la dinámica de los cúmulos, ejercen varios fenómenos de interés astrofísico tales como el campo gravitatorio galáctico, la pérdida de masa causada por la evolución estelar y la presencia de binarias primordiales. En este trabajo se presentan los resultados de más de un centenar de modelos de cúmulos, con poblaciones comprendidas en el intervalo [100, 10.000] estrellas, calculados con NBODY5, un programa estándar en el campo de la Dinámica Estelar Experimental. De los mismos se deduce que el mecanismo principal que rige la dinámica de los cúmulos abiertos es la evolución estelar de su distribución de masas, encontrándose un comportamiento diferencial fuertemente dependiente de la población inicial del cúmulo. Los mecanismos responsables de la desintegración de los cúmulos abiertos son analizados en detalle, proponiéndose un modelo analítico que ajusta razonablemente los resultados de las simulaciones. Además, y con el fin de explicar el elevado número de sistemas múltiples observados en cúmulos abiertos, se estudian los efectos que, sobre los mismos, ejerce la presencia de una cierta fracción de binarias primordiales. El papel de las binarias tambiín depende de la riqueza del cúmulo y sus efectos se interrelacionan con aquellos debidos a la evolución estelar. Por último, se

  18. Integration of GPS and DinSAR for Deformation Monitoring Based on Ant Colony Optimization

    NASA Astrophysics Data System (ADS)

    Shi, Guoqiang; He, Xiufeng; Xiao, Ruya

    2014-11-01

    To acquire three-dimensional earth surface deformation, a measurement method based on ant colony optimization (ACO) is proposed. It highly integrates high-accuracy GPS observations from sparse ground points with InSAR line-of-sight (LOS) direction information. Two constraints, GPS and DInSAR observations, are employed in constructing the energy function whose minimum value will be searched by the ACO operated in continuous space. Compared with conventional interpolation algorithms, the proposed method increases the three-dimensional deformation observation accuracy, especially showing the improvement in the up direction.

  19. [Quality assurance in student training. Prerequisites for DIN EN ISO 9001:2000 in teaching].

    PubMed

    Ochsner, W; Kaiser, C; Schirmer, U

    2007-07-01

    Standards of quality assurance according to DIN EN ISO 9001:2000 have been implemented in many university hospital departments, but often teaching activities are not included. This work presents a method that allows, after having defined the various teaching activities as sub-processes of one single core process, to include the manifold teaching activities of university hospital departments into the certification process. The stepwise description of the prerequisites for including teaching activities into ISO 9001 certification is illustrated by a concrete implementation example. PMID:17297598

  20. DIN retention-transport through four hydrologically connected zones in a headwater catchment of the Upper Mississippi River

    USGS Publications Warehouse

    Triska, F.J.; Duff, J.H.; Sheibley, R.W.; Jackman, A.P.; Avanzino, R.J.

    2007-01-01

    Dissolved inorganic nitrogen (DIN) retention-transport through a headwater catchment was synthesized from studies encompassing four distinct hydrologic zones of the Shingobee River Headwaters near the origin of the Mississippi River. The hydrologic zones included: (1) hillslope ground water (ridge to bankside riparian); (2) alluvial riparian ground water; (3) ground water discharged through subchannel sediments (hyporheic zone); and (4) channel surface water. During subsurface hillslope transport through Zone 1, DIN, primarily nitrate, decreased from ???3 mg-N/l to <0.1 mg-N/l. Ambient seasonal nitrate:chloride ratios in hillslope flow paths indicated both dilution and biotic processing caused nitrate loss. Biologically available organic carbon controlled biotic nitrate retention during hillslope transport. In the alluvial riparian zone (Zone 2) biologically available organic carbon controlled nitrate depletion although processing of both ambient and amended nitrate was faster during the summer than winter. In the hyporheic zone (Zone 3) and stream surface water (Zone 4) DIN retention was primarily controlled by temperature. Perfusion core studies using hyporheic sediment indicated sufficient organic carbon in bed sediments to retain ground water DIN via coupled nitrification-denitrification. Numerical simulations of seasonal hyporheic sediment nitrification-denitrification rates from perfusion cores adequately predicted surface water ammonium but not nitrate when compared to 5 years of monthly field data (1989-93). Mass balance studies in stream surface water indicated proportionally higher summer than winter N retention. Watershed DIN retention was effective during summer under the current land use of intermittently grazed pasture. However, more intensive land use such as row crop agriculture would decrease nitrate retention efficiency and increase loads to surface water. Understanding DIN retention capacity throughout the system, including special channel

  1. Diné (Navajo) parents' and community leaders' perceptions of agriculture-related injury risk to youth: a social narrative.

    PubMed

    Shumway, K; Pate, M L; McNeal, L G

    2014-01-01

    The purpose of this study was to provide a formative needs assessment of Diné (Navajo) parents for the prevention of childhood injuries resulting from livestock and horses. The research objectives were to identify parents' perceived livestock and horse related injury risks to Diné children and describe Diné community stakeholder input on prevention interventions for reducing injury risks to children associated with livestock and horse related activities on farms or ranches. The assessment used a survey constructed of closed and open-response questions to gauge Diné farmers' and ranchers' perceptions of injury risks to children who live or work on agricultural operations. Additional questions were asked to gauge Diné acceptance of an online training program as a prevention intervention to reduce livestock and horse related injuries to children. A total of 96 individuals agreed to participate in the survey and provided usable responses. A total of 53.2% (f = 50) of participants were female. Sixty-three percent of participants (f = 58) perceived that youth who work with intact male livestock were at high risk for injury. A total of 25 individuals perceived that youth who ride horses without equestrian helmets were at high risk for injury. Approximately 96% (f = 89) of those surveyed agreed or strongly agreed that they would use an online training program to promote agricultural health and safety for Diné youth. When participants were asked if there were safety issues associated with having youth working on a farm or ranch, a very large portion felt that the biggest issue was a lack of education and instruction from elders. A recommendation for an injury prevention practice included developing a user-friendly online network, giving parents and community leaders access to resources to assist in educating youth in local agricultural traditions integrated with safety training. PMID:24804462

  2. [Update of DIN 19.643--treatment and disinfection of swimming pool and bathing tub water].

    PubMed

    Hässelbarth, U

    1992-08-01

    German Standards Specification DIN 19,643 is at present under revision for health reasons and because of both negative and positive experiences gathered in practice. To enable adaptation of the standards specification to future developments, a Part I of the specification is being created comprising the demands to be made on the quality of the water and general demands on the construction and operation of swimming pools and tubs and basins in bath houses, e.g. in spas or municipal swimming pools. The subsequent parts of the new specification (Part 2 to Part n) concern the demands to be made on individual combinations of processes; these can be supplemented at any time in accordance with technical progress without requiring revision of the entire standards specification. Essential innovations are the reformulation of the required efficiency of disinfection, the introduction of the parameters Legionella pneumophila, trihalogen methane (THM) and the reduction of the limit value for chloramines. Technically speaking, the new features concern the automatic measurement of the auxiliary parameters of hygiene such as redox potential, pH value and free chlorine, automatic control of disinfectant additions, automatic filter rinsing with fluidization of the filter-bed to a prescribed minimum bed expansion, and the sight-glas at the filter container. The demands made on Jacuzzi and warm water spouted bed besins are integrated into the specification, thus obviating the need for German Standards Specification DIN 19,644. PMID:1392275

  3. Monthly migration of a tectonic seismic swarm detected by DInSAR: southwest Peloponnese, Greece

    NASA Astrophysics Data System (ADS)

    Kyriakopoulos, Christodoulos; Chini, Marco; Bignami, Christian; Stramondo, Salvatore; Ganas, Athanassios; Kolligri, Maria; Moshou, Alexandra

    2013-09-01

    In the period 2011 June-October, a tectonic swarm of nearly 1222 earthquakes occurred in the Messenia prefecture at the southwestern region of the Peloponnese Peninsula. The swarm happened in the Messenia's Upper Quaternary basin, 25 km NW of the city of Kalamata, and migrated from NNW towards SSE. The largest earthquakes occurred in 2011 August 14 (Mw = 4.8), September 14 (Mw = 4.6) and October 10 (Mw = 4.7), caused moderate structural damages mainly in old houses in four villages and produced particular unrest to the local population. We have investigated the monthly migration of the swarm using Differential Synthetic Aperture Radar Interferometry (DInSAR), presenting for the first time a very close look at the deformation evolution that may reveal an aseismic slip component of the total movement. The geodetically derived slip distribution for the first 4 months revealed that slip migrated laterally along strike (north to south) and vertically from a deep portion, at ˜2.8 km depth, to a shallow portion, at less than 0.5 km, of the fault plane, and concluded its migration towards the surface with a very shallow Mw 4.7 event of 2011 October 10 surprisingly detected by DInSAR.

  4. Expression and Mutational Analysis of DinB-Like Protein DR0053 in Deinococcus radiodurans

    PubMed Central

    Appukuttan, Deepti; Seo, Ho Seong; Jeong, Sunwook; Im, Sunghun; Joe, Minho; Song, Dusup; Choi, Jungjoon; Lim, Sangyong

    2015-01-01

    In order to understand the mechanism governing radiation resistance in Deinococcus radiodurans, current efforts are aimed at identifying potential candidates from a large repertoire of unique Deinococcal genes and protein families. DR0053 belongs to the DinB/YfiT protein family, which is an over-represented protein family in D. radiodurans. We observed that dr0053 transcript levels were highly induced in response to gamma radiation (γ-radiation) and mitomycin C (MMC) exposure depending on PprI, RecA and the DrtR/S two-component signal transduction system. Protein profiles demonstrated that DR0053 is a highly induced protein in cultures exposed to 10 kGy γ-radiation. We were able to determine the transcriptional start site of dr0053, which was induced upon irradiation, and to assign the 133-bp promoter region of dr0053 as essential for radiation responsiveness through primer extension and promoter deletion analyses. A dr0053 mutant strain displayed sensitivity to γ-radiation and MMC exposure, but not hydrogen peroxide, suggesting that DR0053 helps cells recover from DNA damage. Bioinformatic analyses revealed that DR0053 is similar to the Bacillus subtilis protein YjoA, which is a substrate of bacterial protein-tyrosine kinases. Taken together, the DNA damage-inducible (din) gene dr0053 may be regulated at the transcriptional and post-translational levels. PMID:25706748

  5. Sentinel-1 DInSAR processing chain within Geohazard Exploitation Platform

    NASA Astrophysics Data System (ADS)

    Zinno, Ivana; Bonano, Manuela; Buonanno, Sabatino; Casu, Francesco; De Luca, Claudio; Fusco, Adele; Lanari, Riccardo; Manunta, Michele; Manzo, Mariarosaria; Ojha, Chandrakanta; Pepe, Antonio

    2016-04-01

    The Sentinel-1A (S1A) satellite has been launched on April 2014 to acquire SAR data in continuity with the ERS-1/2 and ENVISAT missions. It presents advanced characteristics in terms of revisit time, spatial coverage and service reliability. Such a satellite will be paired during 2016 with the Sentinel-1B twin system that will reduce the constellation revisit time from 12 to 6 days. Accordingly, a huge and ever-increasing data flow relevant to extended areas on Earth will be delivered with a "free and open access" data policy. The S1-A sensor is equipped with a C-band SAR instrument that is conceived for interferometric applications, thus allowing us to analyze Earth's surface displacements through the Differential SAR Interferometry (DInSAR) technique. In particular, S1A SAR data are collected through the Terrain Observation by Progressive Scans (TOPS) mode, which generates Interferometric Wide Swath (IWS) acquisitions. To properly handle S1A TOPS data, the existing DInSAR processing chains have to be adapted with new procedures, which properly take into account the characteristics of this new acquisition mode. Furthermore, another critical point to be taken into account in designing a S1A DInSAR processing chain is the achievement of a good computational efficiency. Indeed, the capability to process in reduced time frames the huge data stream expected by S1A (and, very soon, also by S1B) is a key aspect to fully exploit S1 data archives. In this work we present an efficient interferometric processing chain, based on the advanced DInSAR algorithm referred to as Parallel Small BAseline Subset (P-SBAS), for the generation of S1A IWS surface deformation time-series. It ingests the Single Look Complex data and generates, in unsupervised way, interferograms and displacement time-series. This processing chain is able to exploit distributed computing architectures taking advantage of both multi-node and multi-threading programming techniques. The proposed S1A P

  6. Surface deformation analysis of the Istanbul (Turkey) city retrieved via the SBAS-DInSAR technique

    NASA Astrophysics Data System (ADS)

    Manzo, Mariarosaria; Lanari, Riccardo; Manconi, Andrea; Solaro, Giuseppe; Walter, Thomas R.; Zschau, Jochen

    2010-05-01

    Remote sensing techniques make possible to collect information on dangerous or inaccessible areas. In this context, space-based geodetic techniques as Differential SAR Interferometry (DInSAR) have been used in several areas in order to obtain co-seismic surface deformation maps in a few days or even hours following large earthquakes. The retrieved surface deformation can be used to constrain analytical and/or numerical models to derive parameters such as depth, dimension, orientation, and slip occurred on the fault plane. Moreover, advanced DInSAR techniques allow observing the temporal evolution of the surface displacements, and are thus able to furnish a picture of the pre-seismic state as well as of the post-seismic effects. In this work, we present DInSAR analyses of the deformation patterns in areas that are affected by seismic hazard. In particular, we focus on ground displacements occurred in the greater city area of Istanbul (Turkey) in the last 17 years. To this aim, we exploited the advanced DInSAR algorithm, referred to as Small BAseline Subset (SBAS) technique (Berardino et al., 2002). Displacement time series are retrieved from data acquired by the ERS-1/2 and ENVISAT (Pepe et al., 2005) satellites of the European Space Agency (ESA) between 1992 and 2009. First, we show the results of an analysis carried out at a spatial resolution of about 100 x 100 m. This shows, in addition to the co-seismic displacement caused by the Izmit earthquake (17 August 1999) and associated events, several areas that are subject to subsidence (often in excess of 10 cm per decade) within the city of Istanbul. Moreover, in selected case studies, we further exploited localized phenomena by "zooming" in the areas of interest and carrying out analyses at full spatial resolution scale (10 x 10 m) (Lanari et al., 2004). This additional effort allowed us to discriminate among deformation phenomena occurring at regional and at the building scale. This study provides an updated

  7. Railway deformation detected by DInSAR over active sinkholes in the Ebro Valley evaporite karst, Spain

    NASA Astrophysics Data System (ADS)

    Galve, J. P.; Castañeda, C.; Gutiérrez, F.

    2015-06-01

    Previously not measured subsidence on railway tracks was detected using DInSAR displacement maps produced for the central sector of Ebro Valley (NE Spain). This area is affected by evaporite karst and the analyzed railway corridors traverse active sinkholes that produce deformations in these infrastructures. One of the railway tracks affected by slight settlements corresponds to the Madrid-Barcelona high-speed line, a transport infrastructure highly vulnerable to ground deformation processes. Our analysis based on DInSAR measurements and geomorphological surveys indicate that this line show dissolution-induced subsidence and compaction of anthropogenic deposits (infills and embankments). By using DInSAR techniques, it was also measured the significant subsidence related to the activity of sinkholes in the Castejón-Zaragoza conventional railway line. Thus, this study demonstrate that DInSAR velocity maps coupled with detailed geomorphological surveys may help in the identification of the sectors of railway tracks that may compromise the safety of travellers.

  8. Unsupervised DInSAR processing chain for multi-scale displacement analysis

    NASA Astrophysics Data System (ADS)

    Casu, Francesco; Manunta, Michele

    2016-04-01

    Earth Observation techniques can be very helpful for the estimation of several sources of ground deformation due to their characteristics of large spatial coverage, high resolution and cost effectiveness. In this scenario, Differential Synthetic Aperture Radar Interferometry (DInSAR) is one of the most effective methodologies for its capability to generate spatially dense deformation maps at both global and local spatial scale, with centimeter to millimeter accuracy. DInSAR exploits the phase difference (interferogram) between SAR image pairs relevant to acquisitions gathered at different times, but with the same illumination geometry and from sufficiently close flight tracks, whose separation is typically referred to as baseline. Among several, the SBAS algorithm is one of the most used DInSAR approaches and it is aimed at generating displacement time series at a multi-scale level by exploiting a set of small baseline interferograms. SBAS, and generally DInSAR, has taken benefit from the large availability of spaceborne SAR data collected along years by several satellite systems, with particular regard to the European ERS and ENVISAT sensors, which have acquired SAR images worldwide during approximately 20 years. Moreover, since 2014 the new generation of Copernicus Sentinel satellites has started to acquire data with a short revisit time (12 days) and a global coverage policy, thus flooding the scientific EO community with an unprecedent amount of data. To efficiently manage such amount of data, proper processing facilities (as those coming from the emerging Cloud Computing technologies) have to be used, as well as novel algorithms aimed at their efficient exploitation have to be developed. In this work we present a set of results achieved by exploiting a recently proposed implementation of the SBAS algorithm, namely Parallel-SBAS (P-SBAS), which allows us to effectively process, in an unsupervised way and in a limited time frame, a huge number of SAR images

  9. Dinámica global en galaxias elípticas

    NASA Astrophysics Data System (ADS)

    Pérez, M. J.; Cincotta, P. M.; Giordano, C. M.

    Con el propósito de determinar propiedades dinámicas globales en sistemas triaxiales no integrables, utilizamos una nueva herramienta conocida como ``Mean Exponential Growth factor of Nearby Orbits'' (MEGNO), introducida por Cincotta y Simó (2000). Esta técnica no sólo resulta eficiente para investigar ambas componentes del espacio fase, regular y caótica, sino que también, provee una medida de la hiperbolicidad en el dominio caótico que coincide con la dada por el Lyapunov Characteristic Number (LCN). Previamente este método ha sido aplicado al estudio de modelos simples, y últimamente en el campo de la Mecánica Celeste, al estudio de sistemas planetarios extrasolares. En esta presentación, ilustraremos algunas de las propiedades más importantes del MEGNO, aplic& acute;andolo al potencial tridimensional de Stäckel perturbado.

  10. Unsupervised SBAS-DInSAR time series generation: a small brick for building a Supersites ecosystem

    NASA Astrophysics Data System (ADS)

    Casu, F.; De Luca, C.; Elefante, S.; Imperatore, P.; Lanari, R.; Manunta, M.; Zinno, I.; Farres, J.; Lengert, W.

    2013-12-01

    Differential SAR Interferometry (DInSAR) is an effective tool to detect and monitor ground displacements with centimeter accuracy. The geoscience communities, as well as those related to hazard monitoring and risk mitigation, make extensively use of DInSAR. They take advantage from the current huge amount of SAR data and will benefit the incoming big data stream of Sentinel 1 system. The availability of this information makes possible the generation of Earth's surface displacement maps and time series with large spatial coverage and long time span and, often in conjunction to in-situ data, fosters advances in science. However, the managing, processing and analysis of such a huge amount of data is expected to be the major bottleneck, particularly when crisis phases occur. The emerging need of creating a common ecosystem in which data (space born and in-situ), results and processing tools are shared, is envisaged to be a successful way to address such a problem and contribute to information and knowledge spreading. The Supersites initiative as well as the ESA SuperSites Exploitation Platform (SSEP), through the ESA Grid Processing On Demand (G-POD) and Cloud Computing Operational Pilot (CIOP) projects, provide effective answers to this need. The existent tools for querying and analysing SAR data are required to be redesigned for both processing big data and for quickly replying to simultaneous user requests, mainly during emergency situations. These requirements push for the development of automatic and unsupervised processing tools as well as of scalable, widely accessible and high performance computing capabilities. The cloud-computing environment successfully responds to these objectives, particularly in case of spike and peak requests of processing resources linked to disaster events. In this work we present a parallel computational model for the Small BAseline Subset (SBAS) DInSAR algorithm as it was implemented within the computing environment provided by the

  11. A User-Oriented Methodology for DInSAR Time Series Analysis and Interpretation: Landslides and Subsidence Case Studies

    NASA Astrophysics Data System (ADS)

    Notti, Davide; Calò, Fabiana; Cigna, Francesca; Manunta, Michele; Herrera, Gerardo; Berti, Matteo; Meisina, Claudia; Tapete, Deodato; Zucca, Francesco

    2015-11-01

    Recent advances in multi-temporal Differential Synthetic Aperture Radar (SAR) Interferometry (DInSAR) have greatly improved our capability to monitor geological processes. Ground motion studies using DInSAR require both the availability of good quality input data and rigorous approaches to exploit the retrieved Time Series (TS) at their full potential. In this work we present a methodology for DInSAR TS analysis, with particular focus on landslides and subsidence phenomena. The proposed methodology consists of three main steps: (1) pre-processing, i.e., assessment of a SAR Dataset Quality Index (SDQI) (2) post-processing, i.e., application of empirical/stochastic methods to improve the TS quality, and (3) trend analysis, i.e., comparative implementation of methodologies for automatic TS analysis. Tests were carried out on TS datasets retrieved from processing of SAR imagery acquired by different radar sensors (i.e., ERS-1/2 SAR, RADARSAT-1, ENVISAT ASAR, ALOS PALSAR, TerraSAR-X, COSMO-SkyMed) using advanced DInSAR techniques (i.e., SqueeSAR™, PSInSAR™, SPN and SBAS). The obtained values of SDQI are discussed against the technical parameters of each data stack (e.g., radar band, number of SAR scenes, temporal coverage, revisiting time), the retrieved coverage of the DInSAR results, and the constraints related to the characterization of the investigated geological processes. Empirical and stochastic approaches were used to demonstrate how the quality of the TS can be improved after the SAR processing, and examples are discussed to mitigate phase unwrapping errors, and remove regional trends, noise and anomalies. Performance assessment of recently developed methods of trend analysis (i.e., PS-Time, Deviation Index and velocity TS) was conducted on two selected study areas in Northern Italy affected by land subsidence and landslides. Results show that the automatic detection of motion trends enhances the interpretation of DInSAR data, since it provides an objective

  12. Dinámica y crecimiento de los granos de polvo en la nebulosa protoplanetaria

    NASA Astrophysics Data System (ADS)

    de La Fuente Marcos, Carlos

    2001-06-01

    En el escenario estándar de la formación planetaria, los planetesimales (cuerpos de tamaño kilométrico) crecen a partir de granos de polvo, similares a los interestelares, embebidos en un disco gaseoso denominado nebulosa protoplanetaria. Durante esta etapa, los movimientos del gas pueden tener gran influencia en la dinámica y el crecimiento de los granos de polvo, dado que el flujo kepleriano del gas frena el movimiento de los mismos haciendo que caigan hacia el Sol, y la turbulencia inhibe la inestabilidad gravitacional de la capa de polvo. Aunque se acepta que los planetesimales fueron los elementos constituyentes de los planetas, todavía se desconoce cómo se produjo la formación de los mismos. Por esta razón, en los estudios más recientes, existe un renovado interés por comprender mejor la evolución de la capa de polvo inmersa en el disco gaseoso de la Nebulosa. El gas que fluye en el disco puede engendrar estructuras carentes de simetría axial, como por ejemplo ondas espirales y vórtices, a partir de gran variedad de mecanismos de excitación e inestabilidad. En 1995, Barge y Sommeria pusieron de manifiesto que la existencia de vórtices gaseosos persistentes en la nebulosa solar tendría importantes consecuencias sobre la formación de los planetesimales y el posterior crecimiento de los planetas gigantes. La investigación desarrollada en esta Tesis analiza la relación entre el polvo y el gas debida al acoplamiento por fricción dinámica entre ambos; en concreto, se estudia el efecto del flujo medio del gas sobre la dinámica de las partículas de polvo. El primer objetivo es investigar en profundidad los procesos de captura y crecimiento de los granos de polvo dentro de un vórtice y su posible relevancia en cuanto a la formación de los planetesimales. El segundo objetivo es la exploración de los efectos de ondas espirales propagándose en el disco gaseoso sobre la dinámica y el crecimiento de las partículas. La presencia de líneas de

  13. Detrital control on the release of dissolved organic nitrogen (DON) and dissolved inorganic nitrogen (DIN) from the forest floor under chronic N deposition.

    PubMed

    Park, Ji-Hyung; Matzner, Egbert

    2006-09-01

    The role of detrital quantity and quality in forest floor N leaching was investigated in a litter manipulation experiment at a deciduous forest under chronic N deposition. Dissolved inorganic nitrogen (DIN) comprised the bulk of nitrogen leaching from the control except a short period following autumn litterfall. The dominance of DIN was strengthened by litter exclusion, whereas the addition of glucose or fresh litter led to a small increase in dissolved organic nitrogen (DON) and either a temporary or gradual reduction in NO(3)(-) release, respectively. Changes in soluble organic C and microbial C in the forest floor implied that increased availability of C sources might have enhanced microbial immobilization of DIN, either temporarily following glucose application or over the longer term following litter addition. The results suggest that detrital quantity and quality can play a crucial role in determining the balance between DIN and DON in N-enriched forest soils. PMID:16406164

  14. Spatial and temporal interpolation of DInSAR data at different scales

    NASA Astrophysics Data System (ADS)

    Tessitore, Serena; Fiaschi, Simone; Achilli, Vladimiro; Ahmed, Ahmed; Calcaterra, Domenico; Di Martire, Diego; Guardiola-Albert, Carolina; Meisina, Claudia; Ramondini, Massimo; Floris, Mario

    2015-04-01

    The present study regards the utilization of multi-pass DInSAR algorithms to the ground displacements monitoring at small and large scales. An integration of in situ and DInSAR data to the elaboration of 2D maps of deformation is proposed. A geo-statistical method for "radar-gauge combination" called Ordinary Kriging of Radar Errors (OKRE) has been used. This algorithm uses the punctual values of a primary variable that is represented by measurements of true deformations, whereas radar is comprised as auxiliary information on the spatial distribution (Erdin, 2013). According to this method, is possible to obtain the interpolated map of deformations by subtracting a radar error map from the original interpolated radar map. In particular, the radar error map is carried out by interpolating the differences between radar and in situ data with the OK interpolator. To this aim, in the present work the available standard spirit levelling and GPS data have been used. Moreover, DInSAR data achieved through two different approaches have been taken into account for the spatial analysis and the error map computation at different scales. Specifically, the Persistent Scatterer Technique (PS-InSAR) and the Small BAseline Subset approach (SBAS) have been used to process the ENVISAT SAR images acquired in the period 2002-2010. In the SBAS processing chain, it is possible to activate the Disconnected Blocks tool and perform the SAR data "temporal interpolation". Since the estimation of the results in the processing takes into account the coherence threshold on the input images stack and their connection criteria, only the pixels above the threshold that are fully connected in all the images are solved. By activating the Disconnect Blocks tool, the results are estimated also for those pixels that respect the threshold criteria at least in the 60% of the images even in a not fully connected stack. In this way, the spatial coverage is higher but the reliability of the results is has to

  15. A DinB Ortholog Enables Mycobacterial Growth under dTTP-Limiting Conditions Induced by the Expression of a Mycobacteriophage-Derived Ribonucleotide Reductase Gene

    PubMed Central

    Ghosh, Shreya; Samaddar, Sourabh; Kirtania, Prithwiraj

    2015-01-01

    ABSTRACT Mycobacterium species such as M. smegmatis and M. tuberculosis encode at least two translesion synthesis (TLS) polymerases, DinB1 and DinB2, respectively. Although predicted to be linked to DNA repair, their role in vivo remains enigmatic. M. smegmatis mc2155, a strain commonly used to investigate mycobacterial genetics, has two copies of dinB2, the gene that codes for DinB2, by virtue of a 56-kb chromosomal duplication. Expression of a mycobacteriophage D29 gene (gene 50) encoding a class II ribonucleotide reductase in M. smegmatis ΔDRKIN, a strain derived from mc2155 in which one copy of the duplication is lost, resulted in DNA replication defects and growth inhibition. The inhibitory effect could be linked to the deficiency of dTTP that resulted under these circumstances. The selective inhibition observed in the ΔDRKIN strain was found to be due solely to a reduced dosage of dinB2 in this strain. Mycobacterium bovis, which is closely related to M. tuberculosis, the tuberculosis pathogen, was found to be highly susceptible to gene 50 overexpression. Incidentally, these slow-growing pathogens harbor one copy of dinB2. The results indicate that the induction of a dTTP-limiting state can lead to growth inhibition in mycobacteria, with the effect being maximum in cells deficient in DinB2. IMPORTANCE Mycobacterium species, such as M. tuberculosis, the tuberculosis pathogen, are known to encode several Y family DNA polymerases, one of which is DinB2, an ortholog of the DNA repair-related protein DinP of Escherichia coli. Although this protein has been biochemically characterized previously and found to be capable of translesion synthesis in vitro, its in vivo function remains unknown. Using a novel method to induce dTTP deficiency in mycobacteria, we demonstrate that DinB2 can aid mycobacterial survival under such conditions. Apart from unraveling a specific role for the mycobacterial Y family DNA polymerase DinB2 for the first time, this study also paves

  16. Semi-Automated Detection Of Terrain Activity In The Swiss Alpine Periglacial Environment From DInSAR Scenes

    NASA Astrophysics Data System (ADS)

    Barboux, Chloe; Dalaloye, Reynald; Lambiel, Christophe; Strozzi, Tazio; Raetzo, Hugo; Collet, Claude

    2013-12-01

    This paper proposes a semi-automated method to update inventory of moving slopes. First a Map of Terrain Activity (MTA) is created by partitioning an interferogram, using segmentation and classification processes, into 3 regions: stable areas, coherently moving parts and decorrelated areas (due to motion or not). Then, a Combined-Map of Terrain Activity (C- MTA) is computed describing the general behavior of the terrain at a specific time-lapse. Finally, C-MTA is used to determine the potential change in the activity rate of moving slopes. Tests are performed in a small area using large set of TSX DInSAR scenes from summers 2008 to 2012 in order to update past moving slope inventories produced from ERS DInSAR data.

  17. A general framework and related procedures for multiscale analyses of DInSAR data in subsiding urban areas

    NASA Astrophysics Data System (ADS)

    Peduto, Dario; Cascini, Leonardo; Arena, Livia; Ferlisi, Settimio; Fornaro, Gianfranco; Reale, Diego

    2015-07-01

    In the last decade Differential Synthetic Aperture Radar (DInSAR) data were successfully tested in a number of case studies for the detection, mapping and monitoring of ground displacements associated with natural or anthropogenic phenomena. More recently, several national and regional projects all around the world provided rich data archives whose confident use, however, should rely on multidisciplinary experts in order to avoid misleading interpretations. To this aim, the present work first introduces a general framework for the use of DInSAR data; then, focusing on the analysis of subsidence phenomena and the related consequences to the exposed facilities, a set of original procedures is proposed. By drawing a multiscale approach the study highlights the different goals to be pursued at different scales of analysis via high/very high resolution SAR sensors and presents the results with reference to the case study of the Campania region (southern Italy) where widespread ground displacements occurred and damages of different severity were recorded.

  18. Advanced DInSAR analysis at Campi Flegrei and Vesuvius, Italy

    NASA Astrophysics Data System (ADS)

    Tiampo, K. F.; Camacho, A. G.; Fernandez, J.; Gonzalez, P. J.; Samsonov, S. V.

    2015-12-01

    Geodetic data, the spatial and temporal surface expression of complex geophysical processes in the earth, is being acquired today at unprecedented rates and accuracies. Differential interferometric synthetic aperture radar (DInSAR) is a satellite remote sensing technique used extensively today for mapping ground deformation with high spatial resolution and sub-centimeter precision over large areas that is particularly useful for volcanic monitoring [Massonnet and Feigl, 1998; Rosen et al., 2000]. Here we apply the advanced Multidimensional Small Baseline Subset (MSBAS) InSAR algorithm [Samsonov and d'Oreye, 2012] to several thousand Envisat and RADARSAT-2 images from 1993-2013 and compute time series of ground deformation over the Naples Bay region of Italy. Vesuvius and Campi Flegrei are located in this area in close proximity to the densely populated city of Naples and, as a result, it is one of the most hazardous volcanic areas in the world. We obtain time series of ground deformation at high spatial and temporal resolution that span, for the first time, twenty years. Campi Flegrei underwent continuous subsidence through 1999. Uplift began in 2005, reaching approximately 13 cm by 2013. We model the observed deformation to determine source parameters for subsidence and uplift epochs [Samsonov et al., 2014]. In addition, a typical DInSAR image can contain significant signals from with several different, nonvolcanic sources. For example, we clearly observe decade-long elevation-dependent seasonal oscillations of the vertical displacement component at Vesuvius that are substantially larger than the long-term deformation rate (<0.6 cm/yr). As a result, we employ an eigenpattern decomposition technique known as Karhunen-Loeve expansion (KLE) analysis in order to identify the unique, finite set of correlated deformation patterns associated with volcanic sources at different depths [Tiampo et al., 2004; Tiampo et al., 2012]. Both the inflation and deflation mechanisms

  19. DInSAR and PSI methods for the recognition of landslides: an experience in the Romanian Subcarpathians

    NASA Astrophysics Data System (ADS)

    Floriane, Provost; Jean-Philippe, Malet; Cécile, Doubre; Anne, Puissant; Mihai, Micu

    2015-04-01

    Landslide is one of the common natural hazards in Romania, especially in the Curvature area of the Romanian Subcarpathians. In this region, landslides cause considerable damages to critical infrastructures, build-up environment and cultivated areas. Most of the slopes are affected by translational and rotational landslide types. The objective of this work is to locate and inventory landslides in the Buzau County, and possibly to characterize their dynamics. As the vegetation is abundant in the study area, series of L-band ALOS/PALSAR images are processed using advanced multi-temporal differential SAR interferometry (DInSAR & PSI). To analyze the DInSAR results, an object-oriented segmentation method is proposed to identify possible landslide candidates in the interferograms; to analyze the PSI results, a statistical method is used to identify PS characterized by the same evolution pattern in the time series. Both techniques have proved to be able to detect unrecognised active landslides in the area, and allowed to complete existing geomorphological inventories. Around 700 new areas of landslides were detected after the analysis of the DInSAR results by an expert. However it represents 30% the zones that were detected in total. The false results remained difficult to isolate without a manual assessment. That means that applying SAR interferometry is not a sufficient tool to build exhausting inventories, and depending on the characteristics of the images (frame/track, baseline), the characteristics of the terrain (landcover, slope gradient, geomorphology) and the characteristics of the landslide (size, displacement rate), only a certain percentage (roughly less than 50%) of the already known landslides are detected with InSAR. Moreover, because of the large variety of landcover (urban, forest, cultivated areas, bare soils) and slope morphology in the study area, DInSAR and PSI techniques provide different results depending on local conditions.

  20. Comportamiento dinámico de asteroides en la región 23 < a < 26 UA

    NASA Astrophysics Data System (ADS)

    López García, F.

    Los Kuiper Belt Objects (KBOs) pueden ser una fuente de origen de los cometas de corto período. Muchos de los KBOs observados parecen provenir, al igual que el sistema Pluton-Charonte, de órbitas resonantes con Neptuno. Los Centauros son objetos que dinámicamente provienen de órbitas inestables cuyo semieje mayor se encuentra entre las órbitas de Júpiter y Neptuno. Pudiera ser que estos cuerpos fueron expulsados desde la región del cinturón de Kuiper y se encuentran en un proceso de ``scattering" debido a los efectos gravitacionales de los planetas gigantes. Su tiempo de vida dinámico es de algunos millones de años. La evolución orbital de los objetos trans-Neptunianos nos permite conocer su comportamiento anterior, por eso, es importante su relación dinámica con la evolución orbital de Neptuno. El objeto de este trabajo es estudiar el comportamiento dinámico de partículas test que se encuentran entre las resonancias 3:2 con Neptuno y 2:3 con Urano, las cuales comprenden una región de 2 UA aproximadamente. Los primeros resultados indican que la mayoría de los objetos ficticios son eyectados hacia la parte externa del Sistema Solar, mientras que sólo una parte pequeña del total de objetos estudiados, aproximadamente un 7%, son eyectados hacia la parte interna del Sistema Solar, y en algunos casos sus órbitas pueden cruzar la órbita de los planetas terrestres.

  1. Living in Health, Harmony, and Beauty: The Diné (Navajo) Hózhó Wellness Philosophy

    PubMed Central

    Koithan, Mary

    2015-01-01

    Hózhó is the complex wellness philosophy and belief system of the Diné (Navajo) people, comprised of principles that guide one's thoughts, actions, behaviors, and speech. The alignment of integrative nursing principles and the Hózhó Wellness Philosophy illustrates the power that integrative nursing offers as a meta-theoretical perspective that can transform our healthcare system so that it is inclusive and responsive to the needs of our varied populations. Integrative nursing offers the opportunity to re-introduce cultural wellness wisdom, such as Hózhó, as a means to improve whole-person/whole-systems wellbeing and resilience. Integrative nursing, through the acceptance and validation of indigenous health-sustaining wisdom, contributes to the delivery of effective, authentic, culturally tailored, whole-person/whole-system, patient-centered, relationship-based healthcare. Highlighting the Diné Hózhó philosophy re-introduces this philosophy to the Diné, other American Indian/Alaska Native nations, global indigenous cultures, and even nonindigenous people of the world as a means to promote and sustain global health and wellbeing. PMID:25984415

  2. Living in health, harmony, and beauty: the diné (navajo) hózhó wellness philosophy.

    PubMed

    Kahn-John Diné, Michelle; Koithan, Mary

    2015-05-01

    Hózhó is the complex wellness philosophy and belief system of the Diné (Navajo) people, comprised of principles that guide one's thoughts, actions, behaviors, and speech. The alignment of integrative nursing principles and the Hózhó Wellness Philosophy illustrates the power that integrative nursing offers as a meta-theoretical perspective that can transform our healthcare system so that it is inclusive and responsive to the needs of our varied populations. Integrative nursing offers the opportunity to re-introduce cultural wellness wisdom, such as Hózhó, as a means to improve whole-person/whole-systems wellbeing and resilience. Integrative nursing, through the acceptance and validation of indigenous health-sustaining wisdom, contributes to the delivery of effective, authentic, culturally tailored, whole-person/whole-system, patient-centered, relationship-based healthcare. Highlighting the Diné Hózhó philosophy re-introduces this philosophy to the Diné, other American Indian/Alaska Native nations, global indigenous cultures, and even nonindigenous people of the world as a means to promote and sustain global health and wellbeing. PMID:25984415

  3. El nuevo panorama de la Dinámica Galáctica

    NASA Astrophysics Data System (ADS)

    Pivovaroff, Michael James

    En general, la tendencia imperante en Dinámica Galáctica ha sido considerar que los movimientos estelares son básicamente regulares y que el caos no tiene mayor relevancia en los sistemas estelares. Sin embargo, en el último lustro se vienen acumulando pruebas de la importancia del movimiento caótico en ciertos sistemas estelares que existen en la naturaleza. Por una parte, sobre todo el grupo de David Merritt, lo ha mostrado en los casos de galaxias elípticas con concentraciones centrales de materia; por otra parte, en nuestro propio grupo, lo hemos mostrado para el caso de los satélites galácticos. Las consecuencias de estos hallazgos son tanto de tipo técnico, por la necesidad de construir modelos que contengan órbitas caóticas, como astrofísico, por los efectos del caos sobre la estacionariedad y evolución de los sistemas estelares en los que se presenta.

  4. DInSAR Analysis Reveals Bulging of Azerbaijan Mud Volcano Edifices Before an Eruption

    NASA Astrophysics Data System (ADS)

    Antonielli, Benedetta; Monserrat, Oriol; Bonini, Marco; Righini, Gaia; Sani, Federico; Luzi, Guido

    2015-05-01

    Mud volcanism consists in the surface extrusion of gases, saline waters and mud breccias, which produce conical edifices of various sizes with morphology similar to that of magmatic volcanoes. In this work, DInSAR technique has been used to investigate the ground deformation related to the activity of Azerbaijan mud volcanoes during the period October 2003-November 2005. This work focuses on two important deformation events at the Ayaz-Akhtarma and Khara-Zira mud volcanoes. The ground deformations at mud volcanoes are generally originated by fluid pressure and volume variations in the reservoir. The observed deformation pattern is characterized by pre-eruptive inflation that reaches a cumulative value of up to 20 cm at Ayaz-Akhtarma in about two years. Similar pre-eruptive bulging has been observed at magmatic volcanoes, where uplift is typically associated with magma intrusion. We conclude that mud and magmatic volcanoes display some similarities in the behavior of ground deformation during pre-eruptive stages.

  5. [Oswald Bumke in Leipzig. Beyond Kraepelin, Freud and Rüdin's Entartungslehre].

    PubMed

    Steinberg, H

    2008-03-01

    Oswald Bumke is a central figure in twentieth-century German psychiatry, having had a considerable influence on its development as one of its best-known representatives. His time in Leipzig from 1921 to 1924 and subsequently in Munich up to the mid-1930s saw the second major phase in his scientific work, during which he laid the basis for the renown he still enjoys as a result of his specialist encyclopaedic textbooks and manuals summarising the current knowledge of his time. At the beginning of the 1920s he made his stand on the burning issues of the day and thus influenced the conceptional history of the subject. For instance, he proposed his own philosophical psychology, rejecting the experimental psychological approach of Emil Kraepelin. By challenging the libido theory and the dynamic unconscious he weakened the impact of Sigmund Freud and psychoanalysis on German scientific psychiatry. Moreover, Bumke strongly opposed the prevailing concept of degeneration and its main protagonist Ernst Rüdin. Owing to the political and social developments at the time, however, he was not able to raise much support among his colleagues. PMID:17985109

  6. Plio-Quaternary reactivation of the Neogene margin off NW Algiers, Algeria: The Khayr al Din bank

    NASA Astrophysics Data System (ADS)

    Yelles, Abdelkarim; Domzig, Anne; Déverchère, Jacques; Bracène, Rabah; Mercier de Lépinay, Bernard; Strzerzynski, Pierre; Bertrand, Guillaume; Boudiaf, Azzedine; Winter, Thierry; Kherroubi, Abdelaziz; Le Roy, Pascal; Djellit, Hamou

    2009-09-01

    The Algiers region, northern Algeria, is known to be seismically active, with recurrent large (M>6) earthquakes. Because of the lack of high-resolution bathymetry, the offshore structures remained for a long time poorly known. Thanks to a new marine data base (MARADJA 2003 cruise), the offshore part of the margin is accurately mapped, and new active and recent structures are described. West of the bay of Algiers, the margin enlarges, forming the Khayr al Din bank, interpreted as a tilted block of the passive margin born during the opening of the Algero-Provençal basin. At the slope break, a 80 km-long fault-tip Quaternary fold, namely the Khayr al Din fault, extends at the foot of the margin off NW Algiers and represents the largest active structure of the coastal area, together with the Sahel anticline. We also map for the first time a set of overlapping, en echelon active folds in the upper part of the Khayr al Din bank, located off previously known active structures on land. Most of these faults represent actually a threat for the Algiers region in terms of seismic hazard but also geological hazards, such as tsunamis, as most of them depicts significant dimensions and slip rates. The highest long-term horizontal shortening rate is found on the Khayr al Din fault and is estimated at 0.5 ± 0.1 mm/yr, with a maximal magnitude of 7.3, which provides one of the highest seismogenic potential in the region. A new tectonic framework for the Algiers region is proposed, in which the main south-dipping offshore structure, of opposite vergence relative to most thrusts on land, appears to be nowadays the main driving fault system, as also found further east in the Boumerdès (M 6.8) 2003 rupture zone. The overall apparent pop-up structure of the recent and active faults may result from a progressive migration of the plate limit from the Late Miocene, north-dipping suture zone on land, to the Quaternary, south-dipping main Khayr al Din fault at sea, suggesting a process of

  7. Fully exploitation of SBAS-DInSAR deformation time series for assessing structural damage: the case study of Rome, Italy

    NASA Astrophysics Data System (ADS)

    Bonano, Manuela; Arangio, Stefania; Calò, Fabiana; Di Mauro, Maria; Marsella, Maria; Manunta, Michele

    2014-05-01

    Remote sensing techniques have demonstrated to be effective tools to support natural and man-made risk mitigation activities. Among these, the Differential Synthetic Aperture Radar (SAR) Interferometry (DInSAR) technology is largely exploited in geoscience, oil and gas extraction, and landslide fields. Recently, thanks to the large availability of high resolution SAR systems (10 m or less), as well as to the development of advanced data processing techniques, DInSAR products have also started to be effectively used for applications in urban areas to detect localized displacements affecting single buildings and infrastructures. The advanced DInSAR technique referred to as Small Baseline Subset (SBAS) (Lanari et al., 2004) allows us to generate very long deformation time series, by exploiting large SAR datasets spanning up to 20 years (Bonano et al., 2012). Thanks to its capability to investigate wide areas, the SBAS-DInSAR technique is particularly suitable to remotely analyse the structural conditions of buildings located in densely urbanized zones. In this work, we fully exploit the results achieved over the city of Rome, Italy, through the well-established SBAS-DInSAR approach, aimed at performing a quantitative assessment of structural damage in urban areas affected by ground deformation (Arangio et al., 2013). More in details, we present an innovative methodology that integrates the SBAS-DInSAR measurements within an existing model, in order to assess the damage, and possibly estimate the future structural conditions, of single buildings affected by significant foundation settlements. In particular, a semi-empirical approach, based on a laminated beam model (Finno et al., 2005), is applied to investigate the damage of buildings located in the southern part of the city. The obtained results are in substantial agreement with in situ surveys, proving that the presented approach is an effective tool for the preliminary evaluation of the structural conditions in

  8. Two host-induced Ralstonia solanacearum genes, acrA and dinF, encode multidrug efflux pumps and contribute to bacterial wilt virulence.

    PubMed

    Brown, Darby G; Swanson, Jill K; Allen, Caitilyn

    2007-05-01

    Multidrug efflux pumps (MDRs) are hypothesized to protect pathogenic bacteria from toxic host defense compounds. We created mutations in the Ralstonia solanacearum acrA and dinF genes, which encode putative MDRs in the broad-host-range plant pathogen. Both mutations reduced the ability of R. solanacearum to grow in the presence of various toxic compounds, including antibiotics, phytoalexins, and detergents. Both acrAB and dinF mutants were significantly less virulent on the tomato plant than the wild-type strain. Complementation restored near-wild-type levels of virulence to both mutants. Addition of either dinF or acrAB to Escherichia coli MDR mutants KAM3 and KAM32 restored the resistance of these strains to several toxins, demonstrating that the R. solanacearum genes can function heterologously to complement known MDR mutations. Toxic and DNA-damaging compounds induced expression of acrA and dinF, as did growth in both susceptible and resistant tomato plants. Carbon limitation also increased expression of acrA and dinF, while the stress-related sigma factor RpoS was required at a high cell density (>10(7) CFU/ml) to obtain wild-type levels of acrA expression both in minimal medium and in planta. The type III secretion system regulator HrpB negatively regulated dinF expression in culture at high cell densities. Together, these results show that acrAB and dinF encode MDRs in R. solanacearum and that they contribute to the overall aggressiveness of this phytopathogen, probably by protecting the bacterium from the toxic effects of host antimicrobial compounds. PMID:17337552

  9. Ground deformation tracking over Mt. Baekdu with DInSAR time series analysis and atmospheric correction

    NASA Astrophysics Data System (ADS)

    Hong, S.; Kim, J.; Lin, S.; yun, H.; Seo, H.; Choi, Y.

    2013-12-01

    Mt. Baekdu (also known as Changbai in Chinese) is a volcanic mountain located on the border between North Korea and China. It made one of the most destructive eruptions in the recorded history around 1000 A.D. This eruption was estimated to produce explosive Volcanic Explosivity Index (VEI) 7 eruption (Yin et al., 2012) which was comparable to Mt. Tambora's eruption. Since making minor eruption in 1702 A.D. as clearly stated in the Korean history, the Mt. Baekdu has been dormant. With continuous monitoring over Mt. Baekdu (Xu et al., 2012), it is evident that the frequencies of earthquakes and gas emission were increasing. The results showed important precursors of volcanic activation, including: (1) Strong seismic activities especially from 2002 to 2006; (2) Abnormal gas emissions in three hot springs around the summit from 2002 to 2006; (3) Strong vertical uplift during 2002 to 2005 and horizontal displacement away from Caldera Lake observed using GPS data; (4) A number of abnormal thermal activities in hot springs; (5) Surface deflation indicating new magma activities at the western and northern slopes from 2009. Therefore, it is realized that periodic magma activities are underway beneath Mt. Baekdu from the ground observations. In addition to such short-term campaigns applied on discrete observation stations, a comprehensive monitoring covering overall extent of Mt. Baekdu was further proposed. The Differential Interferometric Synthetic Aperture Radar (DInSAR) technique employing a series of remote sensed SAR phase angle difference was applied to address the difficulty for direct access to the border area due to political situation in this study. In order to deal with the harsh environmental conditions which might limit a successful D-InSAR processing over Mt. Baekdu, e.g. water vapor, vegetation canopy and steep slope around summit, StaMPS/MTI (Stanford Method for Persistent Scatterers/Multi-Temporal InSAR) approach for detecting time series deformation

  10. DInSAR time series generation within a cloud computing environment: from ERS to Sentinel-1 scenario

    NASA Astrophysics Data System (ADS)

    Casu, Francesco; Elefante, Stefano; Imperatore, Pasquale; Lanari, Riccardo; Manunta, Michele; Zinno, Ivana; Mathot, Emmanuel; Brito, Fabrice; Farres, Jordi; Lengert, Wolfgang

    2013-04-01

    One of the techniques that will strongly benefit from the advent of the Sentinel-1 system is Differential SAR Interferometry (DInSAR), which has successfully demonstrated to be an effective tool to detect and monitor ground displacements with centimetre accuracy. The geoscience communities (volcanology, seismicity, …), as well as those related to hazard monitoring and risk mitigation, make extensively use of the DInSAR technique and they will take advantage from the huge amount of SAR data acquired by Sentinel-1. Indeed, such an information will successfully permit the generation of Earth's surface displacement maps and time series both over large areas and long time span. However, the issue of managing, processing and analysing the large Sentinel data stream is envisaged by the scientific community to be a major bottleneck, particularly during crisis phases. The emerging need of creating a common ecosystem in which data, results and processing tools are shared, is envisaged to be a successful way to address such a problem and to contribute to the information and knowledge spreading. The Supersites initiative as well as the ESA SuperSites Exploitation Platform (SSEP) and the ESA Cloud Computing Operational Pilot (CIOP) projects provide effective answers to this need and they are pushing towards the development of such an ecosystem. It is clear that all the current and existent tools for querying, processing and analysing SAR data are required to be not only updated for managing the large data stream of Sentinel-1 satellite, but also reorganized for quickly replying to the simultaneous and highly demanding user requests, mainly during emergency situations. This translates into the automatic and unsupervised processing of large amount of data as well as the availability of scalable, widely accessible and high performance computing capabilities. The cloud computing environment permits to achieve all of these objectives, particularly in case of spike and peak

  11. How operational Advanced-DInSAR Analysis can improve knowledge on natural and anthropogenic deformations for Nuclear Power Plant areas

    NASA Astrophysics Data System (ADS)

    Vollrath, Andreas; Zucca, Francesco; Stramondo, Salvatore; Bignami, Christian; Roeder, Johannes

    2015-04-01

    The application of Advanced Differential Interferometric Synthetic Aperture Radar (A-DINSAR) techniques has strongly emerged in the last two decades and became an important part in georelated fields. State-of-the-art A-DInSAR methods, such as Persistent Scatterer Interferometry (PSI) or the Small BASeline (SBAS) approach have demonstrated their usefulness in monitoring urban areas and single buildings, up to critical infrastructures. Combined with additional data from GPS networks or levelling, it could prove its large potential for an operational, cost-effective mapping of surface deformations. Given a reasonable amount of images, changes in surface deformation can be detected down to 1 mm/y. Compared to point-wise field measurements it offers a spatially consistent mapping approach from local to regional scales. In this review we want to provide a synopsis how A-DInSAR can be utilized in the framework of Nuclear Power Plant safety. Indeed, A-DInSAR is able to provide a detailed spatial analysis of slow movements occurring at NPP structures directly, as well as within the surrounding areas of the NPPs. Different phenomena of surface motion can be subject of such a monitoring. Natural causes, like active tectonics and terrain instability of slope which lead to landslides, as well as human-induced subsidence phenomena due to heavy construction or water pumping can be detected. We start by presenting techniques to determine the feasibility of the analysis for a given area and show its limitations. Then we propose a short insight into state-of-the-art studies where landslides, interseismic and human-induced deformation of the surface were mapped by A-DInSAR, to point out the relevance of a consequent analysis over an area of a NPP. Furthermore we present results of case studies from international projects (TERRAFIRMA) as well as preliminary results from the Krsko NPP in Slovenia. Finally, we provide a outlook into present and future trends concerning the use of freely

  12. Accuracy improvement of the ice flow rate measurements on Antarctic ice sheet by DInSAR method

    NASA Astrophysics Data System (ADS)

    Shiramizu, Kaoru; Doi, Koichiro; Aoyama, Yuichi

    2015-04-01

    DInSAR (Differential Interferometric Synthetic Aperture Radar) is an effective tool to measure the flow rate of slow flowing ice streams on Antarctic ice sheet with high resolution. In the flow rate measurement by DInSAR method, we use Digital Elevation Model (DEM) at two times in the estimating process. At first, we use it to remove topographic fringes from InSAR images. And then, it is used to project obtained displacements along Line-Of-Sight (LOS) direction to the actual flow direction. ASTER-GDEM widely-used for InSAR prosessing of the data of polar region has a lot of errors especially in the inland ice sheet area. Thus the errors yield irregular flow rates and directions. Therefore, quality of DEM has a substantial influence on the ice flow rate measurement. In this study, we created a new DEM (resolution 10m; hereinafter referred to as PRISM-DEM) based on ALOS/PRISM images, and compared PRISM-DEM and ASTER-GDEM. The study area is around Skallen, 90km south from Syowa Station, in the southern part of Sôya Coast, East Antarctica. For making DInSAR images, we used ALOS/PALSAR data of 13 pairs (Path633, Row 571-572), observed during the period from November 23, 2007 through January 16, 2011. PRISM-DEM covering the PALSAR scene was created from nadir and backward view images of ALOS/PRISM (Observation date: 2009/1/18) by applying stereo processing with a digital mapping equipment, and then the automatically created a primary DEM was corrected manually to make a final DEM. The number of irregular values of actual ice flow rate was reduced by applying PRISM-DEM compared with that by applying ASTER-GDEM. Additionally, an averaged displacement of approximately 0.5cm was obtained by applying PRISM-DEM over outcrop area, where no crustal displacement considered to occur during the recurrence period of ALOS/PALSAR (46days), while an averaged displacement of approximately 1.65 cm was observed by applying ASTER-GDEM. Since displacements over outcrop area are considered

  13. Quality management in a radiological practice: experiences with a certification for DIN EN ISO 9001:2000.

    PubMed

    Kirsch, Michael; Vogg, Ingrid; Hosten, Norbert; Flessa, Steffen

    2010-07-01

    This paper describes the introduction of a total quality management system in a radiological practice. Certification was based on DIN EN ISO 9001:2000. The implementation of the quality management system had to overcome a number of barriers, for instance, legal obligations of a partnership association, leadership problems, and the fear to loose all hindered implementation. The knowledge of these barriers induces a faster and cheaper implementation of a quality management system in a radiological practice as a foundation of improved quality and competitiveness. PMID:19447575

  14. Spatial-temporal surface deformation of Los Angeles over 2003-2007 from weighted least squares DInSAR

    NASA Astrophysics Data System (ADS)

    Hu, Jun; Li, Zhiwei; Ding, Xiaoli; Zhu, Jianjun; Sun, Qian

    2013-04-01

    The spatial-temporal evolution of surface displacement of Los Angeles area over 2003-2007 is measured with weighted least squares (WLS) small baseline (SB) DInSAR technique. 32 small baseline interferograms are generated from 18 SAR images acquired by the ENVISAT satellite and separated into two independent subsets. An additional interferogram with a longer baseline but good interferometric quality is used to link the two subsets. A time series of displacements with their corresponding standard deviations (STD) are derived from the WLS DInSAR solution by considering the interferometric displacement variances when determining the weighting scheme. Both the long-term trends and the seasonal variations of the displacements in the area are determined in the study and validated with GPS measurements from a number of stations of the Southern California Integrated GPS Network (SCIGN). The mean line-of-sight (LOS) displacement velocity map shows up to 3 cm/year of ground motion and up to 10 cm of accumulated displacements in Santa Fe Springs and -8 cm in Pomona over 2003-2007. Seasonal variations are identified in Santa Ana basin, the San Gabriel valley and the Lytle Creek basin, respectively.

  15. Ground deformation monitoring using small baseline DInSAR technique: A case study in Taiyuan City from 2003 to 2009

    USGS Publications Warehouse

    Wu, H.-A.; Zhang, Y.-H.; Chen, X.-Y.; Lu, T.; Du, J.; Sun, Z.-H.; Sun, G.-T.

    2011-01-01

    DInSAR technique based on time series of SAR images has been very popular to monitor ground stow deformation in recent years such as permanent scatterers (PS) method small baseline subsets (SBAS) method and coherent targets (CT) method. By taking advantage of PS method and CT method in this paper small baseline DTnSAR technique is used to investigate the ground deformation of Taiyuan City Shanxi Province from 2003 to 2009 by using 23 ENVISAT ASAR images. The experiment results demonstrate that: (1) during this period four significant subsidence centers have been developed in Taiyuan namely Xiayuan Wujiabu Xiaodian Sunjiazhai. The largest subsidence center is Sunjiazhai with an average subsidence rate of -77. 28 mm/a; (2) The subsidence of the old center Wanbolin has sHowed down. And the subsidence in the northern region has stopped and some areas even rebounded. (3) The change of subsidence centers indicates that the control measures of "closing wells and reducing exploitation" taken by the Taiyuan government has achieved initial effects. (4) The experiment results have been validated with leveling data and the acouracy is 2. 90 mm which shows that the small baseline DInSAR technique can be used to monitor urban ground deformation.

  16. Investigation of Landslide from Deformation - Comparison of High Resolution DEM, Aero Photos Using Long Wave Length DInSAR

    NASA Astrophysics Data System (ADS)

    Wang, Kuo-Lung; Lin, Jun-Tin; Lin, Meei-Ling; Lin, Ching-Wee; Chen, Tien-Chien; Tseng, Chih-Ming; Chen, Hongey; Lin, Sheng-Chi

    2013-04-01

    Landslide is one of the common natural hazards in Taiwan, especially in central Taiwan with high elevation and steep terrain. Rainfall is increasing both in accumulation and intensity after 2000. Severe landslide events after Chi Chi earthquake are including typhoon Toraj, typhoon Mindulle, 69 heavy rainfall, typhoon Sinlaku, and typhoon Morakot. However, these landslides could be unstable or deformed after rainfall and sliding in next events. The major purpose of this study is to find these deformed but not slide out slopes but slip in following events. For this objective, differential interferometry synthetic aperture radar (DInSAR) is adopted and an event after 69 heavy rainfall is selected for this research. The vegetation in Taiwan is very well thus wave length of SAR needs as long as possible to penetrate vegetation to reach ground surface. ALOS PALSAR HH image is used in this research to derive fringe in study area. The Study area ranges from Puli in south and Wulin in north, including May River basin, Lushan hot spring area, Chingjing area, and Tsuiluan area. The result shows appropriate relationship between the founded deformed slope from DInSAR and landslip in following event. Also high resolution DEM and aero photos are used to derive topography and landslide identification. The results also show that the process can be reference to produce landslide susceptibility map.

  17. Characterization of dinY, a new Escherichia coli DNA repair gene whose products are damage inducible even in a lexA(Def) background.

    PubMed Central

    Petit, C; Cayrol, C; Lesca, C; Kaiser, P; Thompson, C; Defais, M

    1993-01-01

    Bacteriophage Mu dX(Ap lac) was used to isolate a mutation in an Escherichia coli lexA(Def) strain representing a previously undescribed gene (dinY) which does not seem to be under the direct control of LexA. The insertion created a dinY::lacZ fusion in which beta-galactosidase expression required a DNA-damaging treatment (UV irradiation or mitomycin) and activable RecA protein. This strain showed a decreased Weigle reactivation of bacteriophage lambda. However, it was fully inducible for UV mutagenesis. Two-dimensional gel electrophoresis analysis identified two spots absent in the mutant which were both UV inducible only in the presence of activated RecA protein (RecA*). This finding suggests that the dinY::lacZ fusion lies in a gene either that is under the direct control of activated RecA or whose product undergoes RecA*-dependent posttranscriptional/posttranslational modification(s). The dinY gene may also control the expression of some other gene(s) and/or lie in an operon. The fusion was mapped at a position between 41 and 41.5 min on the E. coli chromosome, in the vicinity of the ruv operon. Images PMID:8423139

  18. Ernst Rüdin's Unpublished 1922-1925 Study "Inheritance of Manic-Depressive Insanity": Genetic Research Findings Subordinated to Eugenic Ideology.

    PubMed

    Kösters, Gundula; Steinberg, Holger; Kirkby, Kenneth Clifford; Himmerich, Hubertus

    2015-11-01

    In the early 20th century, there were few therapeutic options for mental illness and asylum numbers were rising. This pessimistic outlook favoured the rise of the eugenics movement. Heredity was assumed to be the principal cause of mental illness. Politicians, scientists and clinicians in North America and Europe called for compulsory sterilisation of the mentally ill. Psychiatric genetic research aimed to prove a Mendelian mode of inheritance as a scientific justification for these measures. Ernst Rüdin's seminal 1916 epidemiological study on inheritance of dementia praecox featured large, systematically ascertained samples and statistical analyses. Rüdin's 1922-1925 study on the inheritance of "manic-depressive insanity" was completed in manuscript form, but never published. It failed to prove a pattern of Mendelian inheritance, counter to the tenets of eugenics of which Rüdin was a prominent proponent. It appears he withheld the study from publication, unable to reconcile this contradiction, thus subordinating his carefully derived scientific findings to his ideological preoccupations. Instead, Rüdin continued to promote prevention of assumed hereditary mental illnesses by prohibition of marriage or sterilisation and was influential in the introduction by the National Socialist regime of the 1933 "Law for the Prevention of Hereditarily Diseased Offspring" (Gesetz zur Verhütung erbkranken Nachwuchses). PMID:26544949

  19. The Parallel SBAS-DInSAR algorithm: an effective and scalable tool for Earth's surface displacement retrieval

    NASA Astrophysics Data System (ADS)

    Zinno, Ivana; De Luca, Claudio; Elefante, Stefano; Imperatore, Pasquale; Manunta, Michele; Casu, Francesco

    2014-05-01

    Differential Synthetic Aperture Radar Interferometry (DInSAR) is an effective technique to estimate and monitor ground displacements with centimetre accuracy [1]. In the last decade, advanced DInSAR algorithms, such as the Small Baseline Subset (SBAS) [2] one that is aimed at following the temporal evolution of the ground deformation, showed to be significantly useful remote sensing tools for the geoscience communities as well as for those related to hazard monitoring and risk mitigation. DInSAR scenario is currently characterized by the large and steady increasing availability of huge SAR data archives that have a broad range of diversified features according to the characteristics of the employed sensor. Indeed, besides the old generation sensors, that include ERS, ENVISAT and RADARSAT systems, the new X-band generation constellations, such as COSMO-SkyMed and TerraSAR-X, have permitted an overall study of ground deformations with an unprecedented detail thanks to their improved spatial resolution and reduced revisit time. Furthermore, the incoming ESA Sentinel-1 SAR satellite is characterized by a global coverage acquisition strategy and 12-day revisit time and, therefore, will further contribute to improve deformation analyses and monitoring capabilities. However, in this context, the capability to process such huge SAR data archives is strongly limited by the existing DInSAR algorithms, which are not specifically designed to exploit modern high performance computational infrastructures (e.g. cluster, grid and cloud computing platforms). The goal of this paper is to present a Parallel version of the SBAS algorithm (P-SBAS) which is based on a dual-level parallelization approach and embraces combined parallel strategies [3], [4]. A detailed description of the P-SBAS algorithm will be provided together with a scalability analysis focused on studying its performances. In particular, a P-SBAS scalability analysis with respect to the number of exploited CPUs has

  20. Spatiotemporal analysis and interpretation of 1993-2013 ground deformation at Campi Flegrei, Italy, observed by advanced DInSAR

    NASA Astrophysics Data System (ADS)

    Samsonov, Sergey V.; Tiampo, Kristy F.; Camacho, Antonio G.; Fernández, José; González, Pablo J.

    2014-09-01

    Campi Flegrei is one of the most hazardous volcanic areas in the world because of its close proximity to the city of Naples. Here we apply the multidimensional small baseline subset (MSBAS) differential interferometric synthetic aperture radar (DInSAR) technique to obtain vertical and horizontal components of ground deformation for Campi Flegrei at high spatial and temporal resolutions that span, for the first time, 20 years. The area underwent continuous subsidence from 1993 through 1999. Moderate uplift began in 2010 and substantially increased through 2012, reaching approximately 13 cm by 2013. We model the observed deformation to determine source parameters for subsidence and uplift epochs. Both the inflation and deflation mechanisms involve large, extended sources in a layered hydrothermal system whose location is controlled by the caldera structure and stratigraphy. The temporal resolution of MSBAS approaches that of GPS daily time series, with superior precision and spatial resolution, making it an excellent alternative for volcano monitoring.

  1. Statistical elaborations of PS DInSAR data applied to the analysis of the subsidence affecting the Venetian coast

    NASA Astrophysics Data System (ADS)

    Tessitore, Serena; Fiaschi, Simone; Achilli, Vladimiro; Fabris, Massimo; Floris, Mario

    2015-04-01

    Subsidence is a hazard that may have natural or anthropic origin. It can affect wide areas causing important economic losses. The area of the Venetian coast is affected by subsidence mainly due to the exploitation of its resources (gas and water). In particular, about 40% of the Italian extraction activities are on this area and here the subsidence arise in conjunction with the industrial revolution (1950-1970). An effective monitoring system is necessary for the correct management of the resources exploitation that, very often, is not supervised. To this aim, the innovative DInSAR techniques have been effectively employed as monitoring systems for the risk assessments of various instability phenomena, such as subsidence, landslides, volcanic eruptions, earthquakes, sinkholes. These techniques represent a powerful investigation tool for their high spatial and multi-temporal coverage, fast data acquisition, and overall low costs. Their results are the displacement estimates along the satellite Line of Sight (LOS), the velocity map and the displacements time series for the whole observation period. In the present work, the ERS and ENVISAT DInSAR data, achieved through the Persistent Scatterers (PS) technique, have been used to produce the iso-kinetic maps of deformation for an area of about 4300 Km2. Here, the mean velocities computed along the ERS and ENVISAT observation periods 1992-2000 and 2003-2010 have been interpolated through the Ordinary Kriging (OK) method. Simulated values and estimations of subsidence uncertainties have been analysed to advance the current understanding on the phenomenon that affects the study area. The achieved maps give information about the spatial distribution of the rates of deformation, which could be correlated with the available geological, hydrogeological and geotechnical data, as well as with the recorded damage to the structures and infrastructures. Then, local Authorities can use these maps as useful tools for the monitoring

  2. Small baseline subsets approach of DInSAR for investigating land surface deformation along the high-speed railway

    NASA Astrophysics Data System (ADS)

    Rao, Xiong; Tang, Yunwei

    2014-11-01

    Land surface deformation evidently exists in a newly-built high-speed railway in the southeast of China. In this study, we utilize the Small BAseline Subsets (SBAS)-Differential Synthetic Aperture Radar Interferometry (DInSAR) technique to detect land surface deformation along the railway. In this work, 40 Cosmo-SkyMed satellite images were selected to analyze the spatial distribution and velocity of the deformation in study area. 88 pairs of image with high coherence were firstly chosen with an appropriate threshold. These images were used to deduce the deformation velocity map and the variation in time series. This result can provide information for orbit correctness and ground control point (GCP) selection in the following steps. Then, more pairs of image were selected to tighten the constraint in time dimension, and to improve the final result by decreasing the phase unwrapping error. 171 combinations of SAR pairs were ultimately selected. Reliable GCPs were re-selected according to the previously derived deformation velocity map. Orbital residuals error was rectified using these GCPs, and nonlinear deformation components were estimated. Therefore, a more accurate surface deformation velocity map was produced. Precise geodetic leveling work was implemented in the meantime. We compared the leveling result with the geocoding SBAS product using the nearest neighbour method. The mean error and standard deviation of the error were respectively 0.82 mm and 4.17 mm. This result demonstrates the effectiveness of DInSAR technique for monitoring land surface deformation, which can serve as a reliable decision for supporting highspeed railway project design, construction, operation and maintenance.

  3. Investigation of subsidence in the Manfredonia Gulf (Southern Italy) through multitemporal DInSAR techniques

    NASA Astrophysics Data System (ADS)

    Triggiani, M.; Refice, A.; Capolongo, D.; Bovenga, F.; Caldara, M.

    2009-04-01

    tourist sea village "Ippocampo". Here, unpublished studies based on ground data indicate average subsidence rates of the order of 0.20 mm/y in the last 125 ka for the inland area next to the village. More recently, height maps issued by the Italian Military Geographic Institute (IGM) in the 1950s report heights a.s.l. of the order of a few m. Observing that today the area is practically at sea level, an average subsidence of the order of tens of mm/y can be inferred for the last 50 years. To gain insight into the recent evolution of these phenomena, we investigate vertical movements on the coastal Tavoliere area through multitemporal differential Interferometric synthetic aperture radar (DInSAR) techniques. We use a persistent scatterers interferometry (PSI) processing methodology [2] to estimate subsidence displacement rates from long temporal series of SAR acquisitions. PSI techniques, first developed at POLIMI [3], allow to retrieve phase information from stacks of co-registered SAR interferograms spanning many years and taken from different directions with large baselines, by restricting the analysis to selected image pixels containing single objects with strong radar backscatter returns. Exploiting the high temporal stability of radar returns from these targets, it is possible to correct the images from spurious phase contributions such as atmospheric phase artefacts and errors in the digital elevation models used to account for topographic InSAR phase. Such stable objects typically coincide with man-made features, so successful applications of PSI techniques are mainly reported over urban centers. We processed a total of 105 SAR images acquired from the ERS-1/2 and ENVISAT satellites, organized in 3 stacks related to both descending (50 ERS-1/2 scenes) and ascending (25 ERS-1/2 and 30 ENVISAT scenes) acquisition geometries. The acquisitions refer to the temporal periods from 1995 to 2000 (ERS) and 2003 to 2008 (ENVISAT), respectively, with a temporal repetition

  4. Volcano deformation analysis based an on-demand DInSAR-GRID system: the SBAS-GPOD solution

    NASA Astrophysics Data System (ADS)

    Manunta, M.; Casu, F.; Cossu, R.; Fusco, L.; Guarino, S.; Lanari, R.; Mazzarella, G.; Sansosti, E.

    2009-04-01

    Differential SAR Interferometry (DInSAR) has already demonstrated to be an effective technique to detect and monitor ground displacements with centimeter accuracy. Moreover, the recent development of advanced DInSAR techniques, aimed at the generation of deformation time series, has led to the exploitation of the large archive of SAR data acquired all over the world, during the last 16 years, by the ERS, ENVISAT and RADARSAT satellites. Among these advanced approaches, we focus on the Small BAseline Subset (SBAS) algorithm that relies on the combination of DInSAR data pairs, characterized by a small separation between the acquisition orbits (baseline), in order to produce mean deformation velocity maps and the corresponding time series, maximizing the coherent pixel density of the investigated area. One of the main capabilities of the SBAS approach is the possibility to work at two spatial resolution scales, thus allowing us to investigate deformation phenomena affecting both extended areas (with resolution of about 100 by 100 m) and selected zones, in the latter case highlighting localized displacements that may affect single structures or buildings (at the full instrument resolution). Similarly to other advanced DInSAR techniques, the SBAS approach requires extended data storage and processing capabilities due to the large amount of data exploited for the generation of the final products. Accordingly, we present in this work the results of the first experiment to "plug" the robustness of the SBAS algorithm into the high computing capability provided by a GRID-based system. In particular, we have exploited the low-resolution SBAS algorithm [1] and the ESA Grid Processing-on-Demand (G-POD) system. This environment is one of the results achieved by the ESA Science and Application Department of Earth Observation Programmes Directorate at ESRIN that focused, following the participation to the DATAGRID project (the first large European Commission funded Grid project

  5. Using DInSAR as a tool to detect unstable terrain areas in an Andes region in Ecuador (South America)

    NASA Astrophysics Data System (ADS)

    Mayorga Torres, Tannia

    2014-05-01

    Using DInSAR as a tool to detect unstable terrain areas in an Andes region in Ecuador (South America) 1. INTRODUCTION Monitoring landslides is a mandatory task in charge on the National Institute of Geological Research (INIGEMM) in Ecuador. It is a small country, supposedly will be faster doing monitoring, but what about its geographic characteristics? Lamentably, due to human and financial resources is not possible to put monitoring systems in unstable terrain areas. However, getting ALOS data to accessible price and using open source software to produce interferograms, could be a first step to know steep areas covered by vegetation and where mass movements are not visible. Under this statement, this study is part of the final research in a master study developed at CONAE during 2009-2011, with oral defense in August 2013. As a new technique used in Ecuador, the study processed radar data from ERS-1/2 and ALOS sensor PALSAR for getting differential interferograms, using ROI_PAC software. Stacking DInSAR is applied to get an average of displacement that indicates uplift and subsidence in the whole radar scene that covers two provinces in the Andes region. 2. PROBLEM Mass movements are present in the whole territory, independently of their magnitude and dynamic (slow or fast), they are a latent threat in winter season specially. There are registers of monitoring, such as two GPS's campaigns and artisanal extensometers, which are used to contrast with DInSAR results. However, the campaigns are shorter and extensometers are no trust on all. 3. METHODOLOGY Methodology has four phases of development: (1) Pre-processing of RAW data; (2) Processing of RAW data in ROI_PAC; (3) Post-processing for getting interferograms in units of cm per year; (4) Analysis of the results and comparison with ground truth. Sandwell & Price (1998) proposed Stacking technique to increase the fringes and decrease errors due to the atmosphere, to average several interferograms. L band penetrates

  6. Ground deformation of the western rift of Corinth observed by means of PSI, SBAS and DInSAR methodologies

    NASA Astrophysics Data System (ADS)

    Elias, Panagiotis; Briole, Pierre

    2013-04-01

    The rift of Corinth has been long identified as a site of major importance in Europe due to its intense tectonic activity. It is one of the world's most rapidly extending continental regions and it has one of the highest seismicity rates in the Euro-Mediterranean region. It produces in average, an earthquake of magnitude 6 per century. The GPS studies conducted since 1990 indicate a north-south extension rate across the rift of ~1.5 cm year-1 around its western termination. Geological evidences show that the south coast of the rift is uplifting whereas the north part is subsiding. The western termination of the rift in the Patras broader area presents a major scientific and socio-economic importance, with the Psathopyrgos and the Rion-Patras faults being located very close to the city of Patras. The first DInSAR studies were carried out using SAR/ERS data after the Ms= 6.2 June 15, 1995 Aigion earthquake and contributed to its characterization. More recently the ground deformation of the area has been measured using a series of ASAR/ENVISAT, PALSAR/ALOS and RASARSAT-2 acquisitions. All datasets were processed by means of PSI (Persistent Scatterers Interferometry), SBAS (Small Baseline SubSet) and DInSAR (Differential Interferometry with SAR) methodologies. In addition to widely used tools (DIAPASON, ROI-PAC, STAMPS e.t.c.) in-house procedures and tools have been developed in order to exploit of the synergy of multiple characteristics/properties (frequency, viewing angles, sides, etc) of the SAR acquisitions aiming to the minimisation of the noise components. We verified the agreement between GPS and PSI/SBAS velocities at the location of the five permanent GPS stations operated in the rift since ten years. Ground deformations are visible at various scales and at various places in the produced PSI/SBAS maps and several known faults exhibit ground deformation around them with no earthquake occurred during the observations period: (a) On 18th and 22nd of January 2010

  7. Inhibitory effect of UvrD and DinG on the replication of ColE1-derived plasmids in Escherichia coli.

    PubMed

    Kang, Nalae; Choi, Eunsil; Kim, Sung-Gun; Hwang, Jihwan

    2015-09-01

    CspA has been identified as a major cold-shock protein in Escherichia coli. CspA binds to RNAs which are abnormally folded at low temperature and then acts as an RNA chaperone unfolding those RNAs. The dramatic expression of cspA at low temperature is contributed by posttranscriptional stability and robust translatability. Interestingly, when cspA mRNA encoding a premature nonsense codon was overexpressed at low temperature, cell growth was completely inhibited. This phenotype was termed LACE (the low temperature-dependent antibiotic effect of truncated cspA expression), and this lethality resulted from exclusive stalling of most ribosomes on mutant cspA mRNAs. In a previous study, we demonstrated that overexpression of the ATP-dependent DNA helicases, UvrD and DinG, suppressed the lethality and ribosome stalling caused by mutant cspA mRNA. In the present study, we attempted to elucidate how these two DNA helicases help recover normal growth under LACE condition. Interestingly, we found that UvrD and DinG appeared to have an ability to down-regulate the replication of pUC-based high copy plasmid. In plasmid copy number tests, the amount of pUC-based plasmid encoding mutant cspA was reduced by 3-10-fold when either UvrD or DinG was expressed. Through a β-galactosidase activity assay, we also confirmed that expression of the lacZα gene inserted into the pUC-based plasmid was significantly reduced due to down-regulation of plasmid replication. Our findings imply that UvrD and DinG, known as non-replicative helicases, play a novel role in the regulation of ColE1-like plasmid replication. PMID:26143370

  8. Developmental and Cell Cycle Quiescence Is Mediated by the Nuclear Hormone Receptor Coregulator DIN-1S in the Caenorhabditis elegans Dauer Larva.

    PubMed

    Colella, Eileen; Li, Shaolin; Roy, Richard

    2016-08-01

    When faced with suboptimal growth conditions, Caenorhabditis elegans larvae can enter a diapause-like stage called "dauer" that is specialized for dispersal and survival. The decision to form a dauer larva is controlled by three parallel signaling pathways, whereby a compromise of TGFβ, cyclic guanosine monophosphate, or insulin/IGF-like signaling (ILS) results in dauer formation. Signals from these pathways converge on DAF-12, a nuclear hormone receptor that triggers the changes required to initiate dauer formation. DAF-12 is related to the vitamin D, liver-X, and androstane receptors, and like these human receptors, it responds to lipophilic hormone ligands. When bound to its ligand, DAF-12 acquires transcriptional activity that directs reproductive development, while unliganded DAF-12 forms a dauer-specifying complex with its interacting protein DIN-1S to regulate the transcription of genes required for dauer development. We report here that din-1S is required in parallel to par-4/LKB1 signaling within the gonad to establish cell cycle quiescence during the onset of the dauer stage. We show that din-1S is important for postdauer reproduction when ILS is impaired and is necessary for long-term dauer survival in response to reduced ILS. Our work uncovers several previously uncharacterized functions of DIN-1S in executing and maintaining many of the cellular and physiological processes required for appropriate dauer arrest, while also shedding light on the coordination of nuclear hormone signaling, the LKB1/AMPK signaling cascade, and ILS/TGFβ in the control of cell cycle quiescence and tissue growth: a key feature that is often misregulated in a number of hormone-dependent cancers. PMID:27260305

  9. Land subsidence in the Cerro Prieto Geothermal Field, 1 Baja California, Mexico, from 1994 to 2005. An integrated analysis of DInSAR, levelingand geological data.

    SciTech Connect

    Sarychikhina, O; Glowacka, E; Mellors, R; Vidal, F S

    2011-03-03

    Cerro Prieto is the oldest and largest Mexican geothermal field in operation and has been producing electricity since 1973. The large amount of geothermal fluids extracted to supply steam to the power plants has resulted in considerable deformation in and around the field. The deformation includes land subsidence and related ground fissuring and faulting. These phenomena have produced severe damages to infrastructure such as roads, irrigation canals and other facilities. In this paper, the technique of Differential Synthetic Aperture Radar Interferometry (DInSAR) is applied using C-band ENVISAR ASAR data acquired between 2003 and 2006 to determine the extent and amount of land subsidence in the Mexicali Valley near Cerro Prieto Geothermal Field. The DInSAR results were compared with published data from precise leveling surveys (1994- 1997 and 1997-2006) and detailed geological information in order to improve the understanding of temporal and spatial distributions of anthropogenic subsidence in the Mexicali Valley. The leveling and DInSAR data were modeled to characterize the observed deformation in terms of fluid extraction. The results confirm that the tectonic faults control the spatial extent of the observed subsidence. These faults likely act as groundwater flow barriers for aquifers and reservoirs. The shape of the subsiding area coincides with the Cerro Prieto pull-apart basin. In addition, the spatial pattern of the subsidence as well as changes in rate are highly correlated with the development of the Cerro Prieto Geothermal Field.

  10. Long-term deformation analysis of historical buildings through the advanced SBAS-DInSAR technique: the case study of the city of Rome, Italy

    NASA Astrophysics Data System (ADS)

    Zeni, G.; Bonano, M.; Casu, F.; Manunta, M.; Manzo, M.; Marsella, M.; Pepe, A.; Lanari, R.

    2011-09-01

    Monitoring of deformation phenomena affecting urban areas and man-made structures is of key relevance for the preservation of the artistic, archaeological and architectural heritage. The differential SAR interferometry (DInSAR) technique has already been demonstrated to be an effective tool for non-invasive deformation analyses over large areas by producing spatially dense deformation maps with centimetre to millimetre accuracy. Moreover, by exploiting long sequences of SAR data acquired by different sensors, the advanced DInSAR technique referred to as the small baseline subset (SBAS) approach allows providing long-term deformation time series, which are strategic for guaranteeing the monitoring of urban area displacements. In this work, we investigate the effectiveness of the two-scale multi-sensor SBAS-DInSAR approach to detect and monitor displacements affecting historical and artistic monuments. The presented results, achieved by applying the full resolution SBAS technique to a huge set of ERS-1/2 and ENVISAT data, spanning the 1992-2010 time interval and relevant to the city of Rome (Italy), show the capability of this approach to detect and analyse the temporal evolution of possible deformation phenomena affecting historical buildings and archaeological sites. Accordingly, our analysis demonstrates the effectiveness of the full resolution multi-sensor SBAS approach to operate as a surface deformation tool for supporting the study and conservation strategies of the historical, cultural and artistic heritage.

  11. Experimental research on the impact of Corbicula fluminea on DIN exchange at a tidal flat sediment-water interface

    NASA Astrophysics Data System (ADS)

    Liu, Jie; Chen, Zhenlou; Xu, Shiyuan; Zheng, Xiangmin

    2007-10-01

    Based on a simulative experiment and a comparison analysis, the effect of bivalve Corbicula fluminea activity on sediment-water exchange of dissolved inorganic nitrogen (DIN) is studied. The areas included three intertidal flat sites of the Changjiang (Yangtze) River estuary in China. The interface exchange flux of ammonium, nitrate and nitrite in the short experiment (6 h) was -46.4-40, -74.8-929.1 and 2.5-14.6 µmol/(m2·h), respectively. It was found that the burrowing activities of C. fluminea increased NH{4/+} and NO{3/-} release from sediments to overlying water in the short-term experiment. During long-term incubation, NH{4/+} and NO{3/-} released in turn from the sediments. At the beginning of incubation, bioturbation by C. fluminea could accelerate NH{4/+} release from sediments 2-17 times in different sites, resulting in stronger nitrification and increased NO{3/-} concentrations in the overlying water. Sediment profile analysis post-incubation shows that organic matter mineralization and sediment-water NH{4/+} exchange had been stimulated by C. fluminea bioturbation and bioirrigation during the experiment. Therefore, C. fluminea activities such as excretion, burrowing, irrigation and turbation can effectively alter nitrogen dynamics and accelerate and stimulate nitrogen exchange and cycling at the sediment-water interface.

  12. La dinámica demográfica y la desigualdad educativa en Méxicoa

    PubMed Central

    Giorguli Saucedo, Silvia E.; Vargas Valle, Eunice D.; Ulloa, Viviana Salinas; Hubert, Celia; Potter, Joseph E.

    2015-01-01

    En este trabajo se presenta una investigación sobre el vínculo entre los procesos educativos y la dinámica demográfica en México. La tradición del pensamiento sobre población y desarrollo ha partido de la hipótesis de que el ritmo de crecimiento poblacional, el tamaño de las familias y la migración influyen sobre la acumulación de capital humano entre la población en edades escolares. Se explora aquí la relación del desempeño educativo de los jóvenes entre 14 y 23 años y la razón de dependencia juvenil, la fecundidad adolescente y la migración interna e internacional; para ello se utilizan datos agregados a escala municipal del año 2000. El análisis incluye indicadores de la oferta educativa municipal basados en estadísticas administrativas de la SEP. PMID:26146427

  13. Pre-eruptive ground deformation of Azerbaijan mud volcanoes detected through satellite radar interferometry (DInSAR)

    NASA Astrophysics Data System (ADS)

    Antonielli, Benedetta; Monserrat, Oriol; Bonini, Marco; Righini, Gaia; Sani, Federico; Luzi, Guido; Feyzullayev, Akper A.; Aliyev, Chingiz S.

    2014-12-01

    Mud volcanism is a process that leads to the extrusion of subsurface mud, fragments of country rocks, saline waters and gases. This mechanism is typically linked to hydrocarbon traps, and the extrusion of this material builds up a variety of conical edifices with a similar morphology to those of magmatic volcanoes, though smaller in size. The Differential Interferometry Synthetic Aperture Radar (DInSAR) technique has been used to investigate the ground deformation related to the activity of the mud volcanoes of Azerbaijan. The analysis of a set of wrapped and unwrapped interferograms, selected according to their coherence, allowed the detection of significant superficial deformation related to the activity of four mud volcanoes. The ground displacement patterns observed during the period spanning from October 2003 to November 2005 are dominated by uplift, which reach a cumulative value of up to 20 and 10 cm at the Ayaz-Akhtarma and Khara-Zira Island mud volcanoes, respectively. However, some sectors of the mud volcano edifices are affected by subsidence, which might correspond to deflation zones that coexist with the inflation zones characterized by the dominant uplift. Important deformation events, caused by fluid pressure and volume variations, have been observed both (1) in connection with main eruptive events in the form of pre-eruptive uplift, and (2) in the form of short-lived deformation pulses that interrupt a period of quiescence. Both deformation patterns show important similarities to those identified in some magmatic systems. The pre-eruptive uplift has been observed in many magmatic volcanoes as a consequence of magma intrusion or hydrothermal fluid injection. Moreover, discrete short-duration pulses of deformation are also experienced by magmatic volcanoes and are repeated over time as multiple inflation and deflation events.

  14. The contribute of DInSAR techniques to landslide hazard evaluation in mountain and hilly regions: a case study from Agno Valley (North-Eastern Italian Alps)

    NASA Astrophysics Data System (ADS)

    De Agostini, A.; Floris, M.; Pasquali, P.; Barbieri, M.; Cantone, A.; Riccardi, P.; Stevan, G.; Genevois, R.

    2012-04-01

    In the last twenty years, Differential Synthetic Aperture Radar Interferometry (DInSAR) techniques have been widely used to investigate geological processes, such as subsidence, earthquakes and landslides, through the evaluation of earth surface displacements caused by these processes. In the study of mass movements, contribution of interferometry can be limited due to the acquisition geometry of RADAR images and the rough morphology of mountain and hilly regions which represent typical landslide-prone areas. In this study, the advanced DInSAR techniques (i.e. Small Baseline Subset and Persistent Scatterers techniques), available in SARscape software, are used. These methods involve the use of multiple acquisitions stacks (large SAR temporal series) allowing improvements and refinements in landslide identification, characterization and hazard evaluation at the basin scale. Potential and limits of above mentioned techniques are outlined and discussed. The study area is the Agno Valley, located in the North-Eastern sector of Italian Alps and included in the Vicenza Province (Veneto Region, Italy). This area and the entire Vicenza Province were hit by an exceptional rainfall event on November 2010 that triggered more than 500 slope instabilities. The main aim of the work is to verify if spatial information available before the rainfall event, including ERS and ENVISAT RADAR data from 1992 to 2010, were able to predict the landslides occurred in the study area, in order to implement an effectiveness forecasting model. In the first step of the work a susceptibility analysis is carried out using landslide dataset from the IFFI project (Inventario Fenomeni Franosi in Italia, Landslide Italian Inventory) and related predisposing factors, which consist of morphometric (elevation, slope, aspect and curvature) and non-morphometric (land use, distance of roads and distance of river) factors available from the Veneto Region spatial database. Then, to test the prediction, the

  15. Using Radar Interferometry (DinSAR) to Evaluate Land Subsidence Caused by Excessive Groundwater Withdrawal in Morocco

    NASA Astrophysics Data System (ADS)

    Durham, M. C.; Milewski, A.; El Kadiri, R.

    2013-12-01

    The combination of natural, anthropogenic, and climate change impacts on the water resources of the Middle East and North Africa (MENA) region has devastated its water resources well beyond its current and projected populations. The increased exploitation of groundwater resources in the past half-century coupled with successive droughts has resulted in the acceleration of subsidence rates in the Souss and Massa basins in Morocco. We have completed a preliminary investigation of these impacts on the Souss and Massa basins (~27,000 km2) in the southwestern part of Morocco. This area is characterized by a semi-arid climate (annual precipitation 70-250 mm/year) with agriculture, tourism, and commercial fishing as the primary economic activities, all of which require availability of adequate freshwater resources. Additionally the primary groundwater aquifer (Plio-Quaternary Plain Aquifer), an unconfined aquifer formed mostly of sand and gravel, is being harvested by >20,000 wells at a rate of 650 MCM/yr., exceeding the rate of recharge by 260 MCM/year. Intense development over the past 50 years has exposed the aquifer to a serious risk of groundwater table drawdown (0.5m-2.5m/yr.), land subsidence, loss of artesian pressure, salinization, salt water intrusions along the coast, and deterioration of water quality across the watershed. Differential Interferometry Synthetique Aperture Radar (DInSAR) was utilized to measure ground subsidence induced by groundwater withdrawal. Land subsidence caused by excessive groundwater extraction was determined using a threefold methodology: (1) extraction of subsidence and land deformation patterns using radar interferometry, (2) correlation of the high subsidence areas within the basins to possible natural and anthropogenic factors (e.g. sea level rise, unconsolidated lithological formations distribution, urbanization, excessive groundwater extraction), and (3) forecasting the future of the Souss and Massa basins over the next century

  16. 3D Faulting Numerical Model Related To 2009 L'Aquila Earthquake Based On DInSAR Observations

    NASA Astrophysics Data System (ADS)

    Castaldo, Raffaele; Tizzani, Pietro; Solaro, Giuseppe; Pepe, Susi; Lanari, Riccardo

    2014-05-01

    We investigate the surface displacements in the area affected by the April 6, 2009 L'Aquila earthquake (Central Italy) through an advanced 3D numerical modeling approach, by exploiting DInSAR deformation velocity maps based on ENVISAT (Ascending and Descending orbits) and COSMO-SkyMed data (Ascending orbit). We benefited from the available geological and geophysical information to investigate the impact of known buried structures on the modulation of the observed ground deformation field; in this context we implemented the a priori information in a Finite Element (FE) Environment considering a structural mechanical physical approach. The performed analysis demonstrate that the displacement pattern associated with the Mw 6.3 main-shock event is consistent with the activation of several fault segments of the Paganica fault. In particular, we analyzed the seismic events in a structural mechanical context under the plane stress mode approximation to solve for the retrieved displacements. We defined the sub-domain setting of the 3D FEM model using the information derived from the CROOP M-15 seismic line. We assumed stationarity and linear elasticity of the involved materials by considering a solution of classical equilibrium mechanical equations. We evolved our model through two stages: the model compacted under the weight of the rock successions (gravity loading) until it reached a stable equilibrium. At the second stage (co-seismic), where the stresses were released through a slip along the faults, by using an optimization procedure we retrieved: (i) the active seismogenic structures responsible for the observed ground deformation, (ii) the effects of the different mechanical constraints on the ground deformation pattern and (iii) the spatial distribution of the retrieved stress field. We evaluated the boundary setting best fit configuration responsible for the observed ground deformation. To this aim, we first generated several forward structural mechanical models

  17. Caractérisations structurale et morphologique des couches minces de CuInS2 et d'In-S "airless spray"

    NASA Astrophysics Data System (ADS)

    Kamoun, N.; Belgacem, S.; Amlouk, M.; Bennaceur, R.; Abdelmoula, K.; Belhadj Amara, A.

    1994-03-01

    We have prepared CuInS2 thin layers by airless spray "S.P.A." in order to use them as an absorber in a photovoltaic cell. The X-ray diffraction analysis has showed that these layers are well crystallized with a privileged (112) principal orientation for a ratio of the concentrations in the pulverized solution x=frac[Cu^I][In^{III]}=1.1. After heat treatment under vacuum the crystallization have been clearly improved. The structural analysis of the thin CuInS2 layers have revealed that a secondary phases of In2S3 and In6S7 are present. Thus we have realized by the same technique thin In-S layers whose structural and morphological properties have been studied. This analysis has showed that the In-S layers are well crystallized for a ratio y=frac[In^{3+]}[S^{2-]}=0.6 in the spray solution. The In-S layers are essentially formed by a β-In2S3 material. Although the In6S7 phase appears to the detriment of β-In2S3 phase for y= 0.75. Nous avons préparé des couches minces de CuInS2, par pulvérisation chimique réactive sans air "P.S.A.", en vue de leur utilisation en tant qu'absorbeur dans un dispositif photovoltaïque. L'analyse par diffraction X a montré que ces couches sont bien cristallisées et que l'orientation principale (112) est privilégiée pour un rapport de concentrations x=frac[Cu^I]{[In^{III}]}=1,1 dans la solution à pulvériser. Après le traitement thermique sous vide la cristallisation est nettement améliorée. L'analyse structurale des couches minces de CuInS2 a révélé que ces couches renferment des phases secondaires d'In2S3 et d'In6S7. Ainsi nous avons réalisé par la même technique "P.S.A.", des couches minces d'In-S dont nous avons étudié les propriétés structurales et morphologiques, Cette analyse a montré que les couches d'In-S sont bien cristallisées. Pour un rapport de concentrations en solution de pulvérisation y=frac[In^{3+]}[S^{2-]}=0,6 les couches d'In-S sont surtout formées du matériau β-In2S3. Alors que la phase In6S7

  18. Monitoring of land subsidence in Ravenna Municipality using two different DInSAR techniques: comparison and discussion of the results.

    NASA Astrophysics Data System (ADS)

    Fiaschi, Simone; Di Martire, Diego; Tessitore, Serena; Achilli, Vladimiro; Ahmed, Ahmed; Borgstrom, Sven; Calcaterra, Domenico; Fabris, Massimo; Ramondini, Massimo; Serpelloni, Enrico; Siniscalchi, Valeria; Floris, Mario

    2015-04-01

    Land subsidence affecting the Ravenna Municipality (Emilia Romagna Region, NE Italy) is one of the best example on how the exploitation of natural resources can affect the environment and the territory. In fact, the pumping of groundwater and the extraction of gas from both on and off-shore reservoirs, started in the 1950s, have caused a strong land subsidence affecting most of the Emilia Romagna territory but in particular the Adriatic Sea coastline near Ravenna. In such area the current subsidence rate, even if lower than in the past, can reach the -2cm/y. Local Authorities have monitored this phenomenon over the years with different techniques: spirit levelling, GPS surveys and, more recently, Interferometric Synthetic Aperture Radar (InSAR) techniques, confirming the critical situation of land subsidence risk. In this work, we present the comparison between the results obtained with two different DInSAR techniques applied to the study of the land subsidence in the Ravenna territory: the Small Baseline Subset (SBAS) and the Coherent Pixel Technique (CPT) techniques. The SBAS works on SARscape software and is based on the Berardino et al., 2002 algorithm. This technique relies on the combination of differential interferograms created from stacks of SAR image pairs that have small temporal and perpendicular baselines. Thanks to the application of several interferograms for every single image, it is possible to obtain high spatial coherence, high data density and more effective error reduction. This allows us to obtain mean velocity maps with good data density even over non-urbanized territories. For the CPT we used the SUBsoft processor based on the algorithm implemented by Mora et al., 2003. CPT is able to extract from a stack of differential interferograms the deformation evolution over wide areas during large time spans. The processing scheme is composed of three main steps: a) the generation of the best interferogram set among all the available images of the

  19. Integrated analysis of differential interferometric synthetic aperture radar (DInSAR) and geological data for measuring deformation movement of Kaligarang fault, Semarang-Indonesia

    NASA Astrophysics Data System (ADS)

    Prasetyo, Y.; Fakhrudin, Warasambi, S. M.

    2016-05-01

    Semarang is one of the densely populated city in Central Java which is has Kaligarang's fault. It is lie in Kaligarang River and across several dense urban settlement. The position of Kaligarang's river itself divides in the direction nearly north-south city of Semarang. The impact of the fault can be seen in severals indication such as a land subsidence phenomenon in Tinjomoyo village area which is make impact to house and road destruction. In this research, we have used combination methods between InSAR, DinSAR and geomorphology (geology data) where is this techniques used to identity the fault area and estimate Kaligarang's fault movement velocity. In fault movement velocity observation, we only compute the movement in vertical with neglect horizontal movement. The data used in this study of one pair ALOS PALSAR level 1.0 which was acquired on June 8, 2007and 10 of September 2009. Besides that third ALOS PALSAR earlier, also used data of SRTM DEM 4th version, is used for the correction of the topography. The use of the three methods already mentioned earlier have different functions. For the lnSAR method used for the establishment of a digital model in Semarang. After getting high models digital city of Semarang, the identification process can be done layout, length, width and area of the Kaligarang fault using geomorphology. Results of such identification can be calculated using the rate of deformation and fault movement. From the result generated DinSAR method of land subsidence rate between 3 em to II em. To know the truth measurement that used DinSAR method, is performed with the decline of validation that measured using GPS. After validating obtained standard deviation of 3,073 em. To estimate the Kaligarang's fault pattern and direction is using the geomorphology method. The results that Kaligarang's is an active fault that has fault strike slip as fault pattern. It makes this research is useful because could be used as an inquick assessment in fault

  20. A model for the abrogation of the SOS response by an SOS protein: a negatively charged helix in DinI mimics DNA in its interaction with RecA

    PubMed Central

    Voloshin, Oleg N.; Ramirez, Benjamin E.; Bax, Ad; Camerini-Otero, R. Daniel

    2001-01-01

    DinI is a recently described negative regulator of the SOS response in Escherichia coli. Here we show that it physically interacts with RecA and prevents the binding of single-stranded DNA to RecA, which is required for the activation of the latter. DinI also displaces ssDNA from a stable RecA–DNA cofilament, thus eliminating the SOS signal. In addition, DinI inhibits RecA-mediated homologous DNA pairing, but has no effect on actively proceeding strand exchange. Biochemical data, together with the molecular structure, define the C-terminal α-helix in DinI as the active site of the protein. In an unusual example of molecular mimicry, a negatively charged surface on this α-helix, by imitating single-stranded DNA, interacts with the loop L2 homologous pairing region of RecA and interferes with the activation of RecA. PMID:11230150

  1. Monitoring of surface deformation in open pit mine using DInSAR time-series: a case study in the N5W iron mine (Carajás, Brazil) using TerraSAR-X data

    NASA Astrophysics Data System (ADS)

    Mura, José C.; Paradella, Waldir R.; Gama, Fabio F.; Santos, Athos R.; Galo, Mauricio; Camargo, Paulo O.; Silva, Arnaldo Q.; Silva, Guilherme G.

    2014-10-01

    We present an investigation of surface deformation using Differential SAR Interferometry (DInSAR) time-series carried out in an active open pit iron mine, the N5W, located in the Carajás Mineral Province (Brazilian Amazon region), using 33 TerraSAR-X (TSX-1) scenes. This mine has presented a historical of instability and surface monitoring measurements over sectors of the mine (pit walls) have been done based on ground based radar. Two complementary approaches were used: the standard DInSAR configuration, as an early warning of the slope instability conditions, and the DInSAR timeseries analysis. In order to decrease the topographic phase error a high resolution DEM was generated based on a stereo GeoEye-1 pair. Despite the fact that a DinSAR contains atmospheric and topographic phase artifacts and noise, it was possible to detect deformation in some interferometric pairs, covering pit benches, road ramps and waste piles. The timeseries analysis was performed using the 31 interferometric pairs, which were selected based on the highest mean coherence of a stack of 107 interferograms, presenting less phase unwrapping errors. The time-series deformation was retrieved by the Least-Squares (LS) solution using an extension of the Singular Value Decomposition (SVD), with a set of additional weighted constrain on the acceleration deformation. The atmospheric phase artifacts were filtered in the space-time domain and the DEM height errors were estimated based on the normal baseline diversity. The DInSAR time-series investigation showed good results for monitoring surface displacement in the N5W mine located in a tropical rainforest environment, providing very useful information about the ground movement for alarm, planning and risk assessment.

  2. Espectroscopia multi-objeto e imageamento de Abell 586 com GMOS - o estado dinâmico de um aglomerado de galáxias com arcos gravitacionais

    NASA Astrophysics Data System (ADS)

    Cypriano, E. S.; Sodrã©, L., Jr.; Kneib, J.-P.; Campusano, L.

    2003-08-01

    Nesse trabalho é apresentado um estudo do conteúdo de massa do aglomerado de galáxias A586 (z = 0.171) bem como sua distribuição espacial. Isso foi feito a partir de dados de espectroscopia multi-objeto e imageamento obtidos com o instrumento GMOS acoplado ao telescópio Gemini-Norte. A massa desse aglomerado foi estudada a partir da posição dos seus arcos gravitacionais (lentes fortes), da medida estatística da distorção na forma das galáxias de fundo (lentes fracas) e através da dispersão de velocidades de uma amostra de cerca de 30 galáxias pertencentes ao aglomerado (teorema do virial). Os dois primeiros métodos não dependem do estado dinâmico do aglomerado, enquanto que o terceiro sim. Desse modo, comparando seus resultados pode-se estimar o grau de relaxamento desse sistema. Dados de raios X presentes na literatura também serão incluídos nessa análise. Nossos resultados preliminares a partir das técnicas de lentes fracas, mostram que, supondo que a distribuição de massa desse aglomerado seja a de uma esfera isotérmica, espera-se que ele apresente uma dispersão de velocidades de 1330+/-78 km s-1, em contradicao com medidas similares feitas por Dahle et al. 2002 que encontram valores tão altos quanto 1680 km s-1. Já o estudo dinâmico resultou numa dispersão de velocidades de ~950 km s-1.

  3. Multitemporal DInSAR and GPS measurements unravel space and time distribution of afterslip following the 2009 Mw 6.3 L'Aquila earthquake

    NASA Astrophysics Data System (ADS)

    D'Agostino, N.; Cheloni, D.; Fornaro, G.; Giuliani, R.; Reale, D.

    2011-12-01

    The inversion of multitemporal DInSAR and GPS measurements unravels the coseismic and postseismic (afterslip) slip distributions associated with the 2009 MW 6.3 L'Aquila earthquake and provide insights into the rheological properties and long-term behaviour of the responsible structure, the Paganica fault. Integration of DInSAR time-series from the COSMO-SkyMed constellation and GPS, offers unprecedented details of the initial (first 190 days) postseismic deformation associated with a normal faulting earthquake. Well-resolved patches of high postseismic slip (10-20 cm) appear to surround the main coseismic patch (maximum slip ≈ 1 m) through the entire seismogenic layer above the hypocenter without any obvious depth-dependent control. Time series of postseismic displacement are well reproduced by an exponential function with best-fit decay constants in the range of 20-40 days. The data are unable to resolve migration of afterslip along the fault. Surface fractures observed along the Paganica fault follow the steepest gradients of postseismic line-of-sight satellite displacements and are consistent with a sudden and delayed failure of the shallow layer in response to upward tapering of slip. The occurrence of afterslip at various levels through the entire seismogenic layer argues against exclusive depth-dependent variations of frictional properties on the fault supporting the hypothesis of significant horizontal frictional heterogeneities. We propose that loading of the main asperity controls the recurrence time interval of 2009-type earthquakes, estimated at ≈ 500 years on the basis of paleoseismological observations. In addition to that, paleoseismological evidence of large (> 0.5 m) coseismic offsets seems to require (1) seismic events with rupture surfaces larger than in 2009, (2) complete failure of the 2009 coseismic and postseismic patches and (3) propagation of coseismic rupture to the surface.

  4. 3D displacements maps of the L'Aquila earthquake by applying SISTEM method to GPS and ENVISAT and ALOS DInSAR data

    NASA Astrophysics Data System (ADS)

    Guglielmino, Francesco; Anzidei, Marco; Briole, Pierre; de Michele, Marcello; Elias, Panagiotis; Nunnari, Giuseppe; Puglisi, Giuseppe; Spata, Alessandro

    2010-05-01

    We present an application of the novel SISTEM (Simultaneous and Integrated Strain Tensor Estimation from geodetic and satellite deformation Measurements) approach [Guglielmino et al., 2009] to obtain a 3D estimation of the ground deformation pattern produced by the April 6, 2009, Mw 6.3 L'Aquila earthquake, the most destructive in the Abruzzo region since the huge 1703 earthquake [Boschi et al., 2000; Chiarabba et al., 2005]. The focal mechanism of the main shock is of normal faulting with NE-SW oriented T-axis [INGV, 2009]. Most of the aftershocks, located by the INGV seismic network, are in the depth range 5÷15 km, depicting a SW dipping fault plane [INGV, 2009]. Field observations [EMERGEO working group, 2009] have identified surface ground cracks with centimeter to decimeters throws over a wide belt running along the Paganica Fault. A closely spaced GPS (Global Positioning System) network was set up in this sector of the Apennines after 1999 [Anzidei et al., 2005] and more than 10 Continuous GPS (CGPS) stations have been operating in this region over the last years. On March 30 2008, INGV installed five GPS receivers on selected benchmarks of the Central Apennine Geodetic Network (CaGeoNet) bordering the L'Aquila basin in order to detect the eventual ground movements during the seismic sequence. These stations were crucial to resolve the near-field co-seismic deformation pattern properly, allowing direct observation of the details of co-seismic displacement related to the main shock. Thanks to the ESA Earth Watching project, which made Envisat data quickly available after their acquisition, we performed a DInSAR (Differential Interferometric Synthetic Aperture Radar) analysis of ascending and descending images sampling the date of the earthquake. In particular, we analyze the descending pair for the interval 27/04/2008 - 12/04/2009 (tbline = 350 days; Bperp = 44m) and the ascending pair for the interval 11/03/2009 - 15/04/2009 (tbline = 35 days; Bperp = 227m

  5. 3D displacements maps of the L'Aquila earthquake by applying SISTEM method to GPS and ENVISAT and ALOS DInSAR data

    NASA Astrophysics Data System (ADS)

    Guglielmino, Francesco; Anzidei, Marco; Briole, Pierre; de Michele, Marcello; Elias, Panagiotis; Nunnari, Giuseppe; Puglisi, Giuseppe; Spata, Alessandro

    2010-05-01

    We present an application of the novel SISTEM (Simultaneous and Integrated Strain Tensor Estimation from geodetic and satellite deformation Measurements) approach [Guglielmino et al., 2009] to obtain a 3D estimation of the ground deformation pattern produced by the April 6, 2009, Mw 6.3 L'Aquila earthquake, the most destructive in the Abruzzo region since the huge 1703 earthquake [Boschi et al., 2000; Chiarabba et al., 2005]. The focal mechanism of the main shock is of normal faulting with NE-SW oriented T-axis [INGV, 2009]. Most of the aftershocks, located by the INGV seismic network, are in the depth range 5÷15 km, depicting a SW dipping fault plane [INGV, 2009]. Field observations [EMERGEO working group, 2009] have identified surface ground cracks with centimeter to decimeters throws over a wide belt running along the Paganica Fault. A closely spaced GPS (Global Positioning System) network was set up in this sector of the Apennines after 1999 [Anzidei et al., 2005] and more than 10 Continuous GPS (CGPS) stations have been operating in this region over the last years. On March 30 2008, INGV installed five GPS receivers on selected benchmarks of the Central Apennine Geodetic Network (CaGeoNet) bordering the L'Aquila basin in order to detect the eventual ground movements during the seismic sequence. These stations were crucial to resolve the near-field co-seismic deformation pattern properly, allowing direct observation of the details of co-seismic displacement related to the main shock. Thanks to the ESA Earth Watching project, which made Envisat data quickly available after their acquisition, we performed a DInSAR (Differential Interferometric Synthetic Aperture Radar) analysis of ascending and descending images sampling the date of the earthquake. In particular, we analyze the descending pair for the interval 27/04/2008 - 12/04/2009 (tbline = 350 days; Bperp = 44m) and the ascending pair for the interval 11/03/2009 - 15/04/2009 (tbline = 35 days; Bperp = 227m

  6. Synergy of ASAR and RADARSAT-2 ultra-fine acquisitions for ground deformation monitoring by means of DInSAR and PSI

    NASA Astrophysics Data System (ADS)

    Elias, Panagiotis; Briole, Pierre; Sykioti, Olga

    2010-05-01

    consideration the raised seismisity of the area. The Psathopyrgos fault zone as well as the Rion-Patras transfer fault zone are investigated for any detectable ground deformations that could be indications/precursors or inter-seismic accumulation processes before a main event. The city of Patras are investigated for any detectable ground/buildings deformation due to human interventions or geophysical processes. The potential of Rion-Antirion bridge monitoring for any detectable deformation as well as the surrounding ground on the two edges of it, are also investigated and assessed. The study area presents major difficulties for DInSAR/PSI applications, due to its intense vegetation coverage and high topography presenting various facets, varying high slopes and shadowing effects. Moreover its intense topography in conjunction with its location between Aegean and Ionian seas is leading to high precipitation rates and extend cloud coverage. All these characteristics of the study area contribute to high decorrelation of the interferometric products. For the estimation of the ocuured deformations a series of ASAR/ENVISAT (image swath 2) data are processed by means of PSI and DInSAR techniques, but RADARSAT-2 (ultra-fine beam mode) data are processed only by means of DInSAR technique due to its lack of historical data. The processing will be carried out exploiting commercial and in-house software. The medium and high ground resolution added value products of the acquired data are combined in the thematic level.

  7. Interplay Between Tectonics And Volcanic Processes Active In The Yellowstone Caldera Detected Via DInSAR And GPS Time-Series

    NASA Astrophysics Data System (ADS)

    Tizzani, Pietro; Battaglia, Maurizio; Castaldo, Raffaele; Pepe, Antonio; Zeni, Giovanni; Lanari, Riccardo

    2014-05-01

    We discriminate and quantify the effects of different stress sources that are active in the Yellowstone volcanic region. In particular, the use of long-term deformation time series allows us to separate the spatial and temporal contributions of the regional tectonic field due to North American (NA) plate motion from the dynamic of magmatic/hydrothermal sources beneath the caldera area. Yellowstone volcano was formed by three major caldera forming eruptions that occurred around 2.0, 1.3 and 0.64 Ma, the most recent one responsible for the 60 km-wide and 40 km-long Yellowstone caldera. Two structural resurgent domes emerged after the last caldera forming eruption: the Mallard Lake (ML) resurgent dome in the southwestern region of Yellowstone caldera, and the Sour Creek (SC) resurgent dome in the northeast part of the caldera. In this work, we extensively exploit DInSAR and GPS measurements to investigate surface deformation at Yellowstone caldera over the last 18 years. We start by analyzing the 1992-2010 deformation time series retrieved by applying the Small BAseline Subset (SBAS) DInSAR technique. This allows us identifying three macro-areas: i) Norris Geyser Basin (NGB), ii) ML and SC resurgent domes and iii) Snake River Plain (SRP), characterized by unique deformation behaviors. In particular, SRP shows a signal related to tectonic deformation, while the other two regions are influenced by the caldera unrest. To isolate the deformation signals related to different stress sources in the Yellowstone caldera, we also remove from the retrieved mean deformation velocity maps the mean displacement rate associated to the northern sector of the Snake River Plain. This latter is the result of tectonic processes controlled by complex interactions between the NA plate, moving in the ENE - WSW direction with a rate of about 2 cm/yr, and the flow of the asthenosphere plume beneath the Yellowstone volcanic region. These de-trended data allow recognizing four major deformation

  8. The dependence of the creep properties of din 1.4970 austenitic stainless steel at 973 K on different thermomechanical pre-treatments

    NASA Astrophysics Data System (ADS)

    Abou Zahra, Ahmed A.; Schroeder, Herbert

    1982-05-01

    The creep-rupture behaviour of a Type DIN 1.4970 austenitic stainless steel has been investigated at 973 K (700°C) in a high vacuum for three conditions of thermomechanical treatment and a wide range of applied stresses using foil specimens of 105 μm thickness. After solution-annealing at 1373 K (1100°C) for all specimens, the three treatments were: — condition 1: 13% cold-worked — condition2: aged for 24 h at 1073 K (800°C) and 13% cold-worked — condition 3: ("Standard condition"): 13% cold-worked and aged for 24 h at 1073 K (800°C). The rupture lives and the minimum creep rates were found to be highly dependent on the applied stresses. The treatment of condition 1 material yielded a product as strong as the "standard" condition 3, while the condition 2 material was less creep resistant. Structural changes as well as fractography were followed using metallographic, transmission and scanning electron microscope techniques. Transgranular ductile fracture was clearly observed in all three conditions. TEM investigations showed that dispersive TiC precipitates were present in the matrix of condition 3 material before creep testing, contrary to condition 1 and 2 material. In condition 1, the TiC dispersion was already found after short creep times, while no dispersive TiC precipitates were found in condition 2 material in any test condition.

  9. Comparison of water-level, extensometric, DInSAR and simulation data for quantification of subsidence in Murcia City (SE Spain)

    NASA Astrophysics Data System (ADS)

    Tessitore, S.; Fernández-Merodo, J. A.; Herrera, G.; Tomás, R.; Ramondini, M.; Sanabria, M.; Duro, J.; Mulas, J.; Calcaterra, D.

    2016-02-01

    Subsidence due to groundwater overexploitation has been recognized in the metropolitan area of Murcia (25 km2) in south-eastern Spain since the early 1990s. Previous published works have focused their attention on land subsidence that occurred during the drought period between 1995 and 2008. This work first analyzes the groundwater recovery that has occurred since 2008 and then determines the kind of associated ground deformation detected by the new extensometric data. Subsequently, subsidence time series are computed on 24 geotechnical boreholes scattered throughout the study area by means of a hydro-mechanical finite element code and a linear-elastic constitutive law. A spatio-temporal interpolation of the numerically modeled surface displacements is performed over the whole domain and compared with extensometers and DInSAR-derived displacement maps in two different periods: the drought period from 2004 to 2008, and the recovery period from 2008 to 2012. In spite of the limited information on the geomechanical parameters characterizing the modelled geological formations, the proposed approach is able to discriminate areas where the soils have an elastic behavior (small differences in the comparisons) or an elasto-plastic behavior (large differences in the comparisons). This zonation enhances the understanding of the subsidence phenomenon in Murcia City and could prevent, from a quantitatively point of view, future severe subsidence due to aquifer overexploitation.

  10. Comparison of water-level, extensometric, DInSAR and simulation data for quantification of subsidence in Murcia City (SE Spain)

    NASA Astrophysics Data System (ADS)

    Tessitore, S.; Fernández-Merodo, J. A.; Herrera, G.; Tomás, R.; Ramondini, M.; Sanabria, M.; Duro, J.; Mulas, J.; Calcaterra, D.

    2016-05-01

    Subsidence due to groundwater overexploitation has been recognized in the metropolitan area of Murcia (25 km2) in south-eastern Spain since the early 1990s. Previous published works have focused their attention on land subsidence that occurred during the drought period between 1995 and 2008. This work first analyzes the groundwater recovery that has occurred since 2008 and then determines the kind of associated ground deformation detected by the new extensometric data. Subsequently, subsidence time series are computed on 24 geotechnical boreholes scattered throughout the study area by means of a hydro-mechanical finite element code and a linear-elastic constitutive law. A spatio-temporal interpolation of the numerically modeled surface displacements is performed over the whole domain and compared with extensometers and DInSAR-derived displacement maps in two different periods: the drought period from 2004 to 2008, and the recovery period from 2008 to 2012. In spite of the limited information on the geomechanical parameters characterizing the modelled geological formations, the proposed approach is able to discriminate areas where the soils have an elastic behavior (small differences in the comparisons) or an elasto-plastic behavior (large differences in the comparisons). This zonation enhances the understanding of the subsidence phenomenon in Murcia City and could prevent, from a quantitatively point of view, future severe subsidence due to aquifer overexploitation.

  11. General design method for 3-dimensional, potential flow fields. Part 2: Computer program DIN3D1 for simple, unbranched ducts

    NASA Technical Reports Server (NTRS)

    Stanitz, J. D.

    1985-01-01

    The general design method for three-dimensional, potential, incompressible or subsonic-compressible flow developed in part 1 of this report is applied to the design of simple, unbranched ducts. A computer program, DIN3D1, is developed and five numerical examples are presented: a nozzle, two elbows, an S-duct, and the preliminary design of a side inlet for turbomachines. The two major inputs to the program are the upstream boundary shape and the lateral velocity distribution on the duct wall. As a result of these inputs, boundary conditions are overprescribed and the problem is ill posed. However, it appears that there are degrees of compatibility between these two major inputs and that, for reasonably compatible inputs, satisfactory solutions can be obtained. By not prescribing the shape of the upstream boundary, the problem presumably becomes well posed, but it is not clear how to formulate a practical design method under this circumstance. Nor does it appear desirable, because the designer usually needs to retain control over the upstream (or downstream) boundary shape. The problem is further complicated by the fact that, unlike the two-dimensional case, and irrespective of the upstream boundary shape, some prescribed lateral velocity distributions do not have proper solutions.

  12. Numerical modelling of slow landslides through the exploitation of DInSAR and inclinometric measurements: the Ivancich case study (Assisi, Central Italy)

    NASA Astrophysics Data System (ADS)

    Castaldo, Raffaele; Lollino, Piernicola; Tizzani, Pietro; Ardizzone, Francesca; Calò, Fabiana; Guzzetti, Fausto; Manunta, Michele; Manzo, Mariarosaria

    2013-04-01

    Risk management of slow landslides requires both the assessment of slope stability conditions and the analysis of the dynamic evolution in terms of displacement, velocity or acceleration during the deformation phase. To this purpose, we analysed the kinematical evolution of a slow landslides through a numerical model implemented in Finite Element Environment (FEE) and compared with monitoring data based on DInSAR interferometry technique and conventional inclinometric investigation. In our study we analyse the Ivancich slow landslide (Assisi, Central Italy). The landslide body is formed of detritus (or debris) material sliding on a arenaceous marl substratum, with a thin shear band detected using borehole and inclinometric data, at depth ranging from 20 to 60 m. Specifically, we considered the active role of this shear band in the control of the landslide evolution process. Ground surface displacement measurements have been also retrieved via an advanced space-based Differential SAR interferometry analysis. ERS-1/2 and ENVISAT SAR images taken in the 1992-2010 period have been used to generate long time-series showing the temporal evolution of surface movements of the unstable slope, that indicate different displacement rates for the various portions of the landslide body. A large field monitoring dataset of the landslide process, including at-depth piezometric and inclinometric measurements acquired in the last decade all over the landslide body, was available to us. The piezometric data do not show appreciable variation of the pore water pressures at the level of the sliding surface. We performed a two-dimensional time-dependent Finite Element Model (FEM) of the active ground deformation field aimed at simulating the kinematical evolution of the different sectors of the unstable mass. In particular, a deviatoric creep law, accounting for a time dependent shear deformation over constant deviatoric stress, has been used to describe the shear band behavior. An

  13. Identification of two DNA helicases UvrD and DinG as suppressors for lethality caused by mutant cspA mRNAs.

    PubMed

    Hwang, Jihwan; Lee, Kangseok; Phadtare, Sangita; Inouye, Masayori

    2012-01-01

    CspA is a major cold shock-inducible protein (70 aa), and its major role in the cold shock response was shown to be as an RNA chaperone destabilizing secondary structure of mRNAs at low temperature. Previously, we showed that the overexpression of mutant cspA containing premature non-sense codons at various positions led to stalled ribosomes on mutant cspA transcripts, ultimately leading to cell death. This lethality is primarily due to the highly translatable cspA 5'-UTR that recruits most of the ribosomes from other mRNAs, which are then stalled at the abnormal stop codon. This was called the 'LACE' effect. We show here that non-sense mutation even at the 67th position as well as substitutions of aromatic amino acid residues present on the RNA-binding surface of CspA protein to alanine caused the LACE effect by trapping a substantial amount of ribosomes on cspA mRNAs. In an attempt to identify a suppressor(s), which may help the cells to recover from the inhibitory LACE effect, genetic screening of an Escherichia coli genomic library was performed. We isolated suppressors that contained the genomic fragments encoding uvrD and dinG, respectively, whose gene products are ATP-dependent DNA helicases. The nucleic acid-binding and ATPase activities of these two helicases were found to be essential for their suppression activity. This genomic screening offers an approach to shed light on the mechanistic of 5'-UTR of cspA mRNA and novel roles of E. coli helicases that function in DNA repair. PMID:22832783

  14. La estructura dinámica del cinturón de Kuiper interior y la formación de Urano y Neptuno

    NASA Astrophysics Data System (ADS)

    Melita, M. D.; Brunini, A.

    De acuerdo a los más recientes estudios sobre la formación de Urano y Neptuno, éstos podrían haber sufrido una importante migración radial hacia el exterior, debido al intercambio de momento angular con los planetesimales presentes en esa región durante las últimas etapas de la formación del Sistema Solar. Esta migración radial habría tenido importantes consecuencias sobre la presente estructura dinámica del cinturón cometario de Kuiper. Tal como lo reflejan las observaciones, la mayoría de los cuerpos observados en el cinturón de Kuiper se encuentran en resonancia de movimientos medios exteriores con Neptuno, hecho que también se refleja en la presente órbita de Plutón. En el presente trabajo se estudia tanto numérica como analíticamente la influencia de la variación de las masas de Urano y Neptuno durante el proceso de migración sobre las probabilidades de captura en algunas de las resonancias exteriores de Neptuno. Se estudia también la influencia de encuentros gravitacionales mutuos sobre la estabilidad de las órbitas resonantes. Este estudio ofrece argumentos de plausibilidad para explicar por qué ninguno de los objetos descubiertos en el cinturón de Kuiper se encontraría en la resonancia 2:1.

  15. Photogrammetry and Remote Sensing: New German Standards (din) Setting Quality Requirements of Products Generated by Digital Cameras, Pan-Sharpening and Classification

    NASA Astrophysics Data System (ADS)

    Reulke, R.; Baltrusch, S.; Brunn, A.; Komp, K.; Kresse, W.; von Schönermark, M.; Spreckels, V.

    2012-08-01

    10 years after the first introduction of a digital airborne mapping camera in the ISPRS conference 2000 in Amsterdam, several digital cameras are now available. They are well established in the market and have replaced the analogue camera. A general improvement in image quality accompanied the digital camera development. The signal-to-noise ratio and the dynamic range are significantly better than with the analogue cameras. In addition, digital cameras can be spectrally and radiometrically calibrated. The use of these cameras required a rethinking in many places though. New data products were introduced. In the recent years, some activities took place that should lead to a better understanding of the cameras and the data produced by these cameras. Several projects, like the projects of the German Society for Photogrammetry, Remote Sensing and Geoinformation (DGPF) or EuroSDR (European Spatial Data Research), were conducted to test and compare the performance of the different cameras. In this paper the current DIN (Deutsches Institut fuer Normung - German Institute for Standardization) standards will be presented. These include the standard for digital cameras, the standard for ortho rectification, the standard for classification, and the standard for pan-sharpening. In addition, standards for the derivation of elevation models, the use of Radar / SAR, and image quality are in preparation. The OGC has indicated its interest in participating that development. The OGC has already published specifications in the field of photogrammetry and remote sensing. One goal of joint future work could be to merge these formerly independent developments and the joint development of a suite of implementation specifications for photogrammetry and remote sensing.

  16. Correlation between pore fluid pressures and DInSAR post-seismic deformation of the May 20, 2012 Emilia-Romagna (Italy) earthquake

    NASA Astrophysics Data System (ADS)

    Moro, M.; Stramondo, S.; Albano, M.; Barba, S.; Solaro, G.; Saroli, M.; Bignami, C.

    2015-12-01

    The present work focuses on the detection and analysis of the postseismic surface deformations following the two earthquakes that hit the Emilia Romagna region (Italy) on May 20 and 29, 2012. The 2012 Emilia earthquake sequence struck the central sector of the Ferrara arc, which represents the external fold-and-thrust system of the Northern Apennines thrust belt buried below the Po plain. The May 20 event occurred on the Ferrara basal thrust at depth, at about 6-7 km, while, during the May 29 event, the rupture jumped on an inner splay of the Ferrara system. The analysis of the postseismic displacements was carried out thanks to a dataset of SAR COSMO­ SkyMed images covering a time span of about one year (May 20, 2012 - May 11, 2013) after the May 20 event. The DInSAR results revealed the presence of two deformation patches: the first one is located in the area that experienced the coseismic uplift. Here the postseismic displacements point out a further ground uplift occurring along the first three months after the 20 May event. The second deformation patch is located in the villages of San Carlo and Mirabello, where ground subsidence lasting about four months was detected. We hypothesized that both the observed phenomena are related to the pore pressure perturbation caused by the coseismic deformation. In particular, the ground uplift is due to the deep crustal deformations caused by the pore fluid diffusion at depth to re-establish the initial hydrostatic stresses. Instead, the ground subsidence is related to the compaction of the shallow sandy layers caused by the liquefaction phenomena, which widely affected the San Carlo and Mirabello area. Preliminary numerical analyses performed with the Finite Element Method and empirical relations confirmed our hypothesis.

  17. Identification of two DNA helicases UvrD and DinG as suppressors for lethality caused by mutant cspA mRNAs

    PubMed Central

    Hwang, Jihwan; Lee, Kangseok; Phadtare, Sangita; Inouye, Masayori

    2012-01-01

    CspA is a major cold-shock inducible protein (70 aa), and its major role in the cold-shock response was shown to be as an RNA chaperone destabilizing secondary structure of mRNAs at low temperature. Previously, we showed that the overexpression of mutant cspA containing premature nonsense codons at various positions led to stalled ribosomes on mutant cspA transcripts, ultimately leading to cell death. This lethality is primarily due to the highly translatable cspA 5′-UTR that recruits most of the ribosomes from other mRNAs, which are then stalled at the abnormal stop codon. This was called the ‘LACE’ effect. We show here that nonsense mutation even at 67th position as well as substitutions of aromatic amino acid residues present on the RNA-binding surface of CspA protein to alanine caused the LACE effect by trapping a substantial amount of ribosomes on cspA mRNAs. In an attempt to identify a suppressor(s), which may help the cells to recover from the inhibitory LACE effect, genetic screening of an E. coli genomic library was performed. We isolated suppressors that contained the genomic fragments encoding uvrD and dinG, respectively, whose gene products are ATP-dependent DNA helicases. The nucleic acid-binding and ATPase activities of these two helicases were found to be essential for their suppression activity. This genomic screening offers an approach to shed light on the mechanistic of 5′-UTR of cspA mRNA and novel roles of E. coli helicases that function in DNA repair. PMID:22832783

  18. 2D Modelling of the Gorkha earthquake through the joint exploitation of Sentinel 1-A DInSAR measurements and geological, structural and seismological information

    NASA Astrophysics Data System (ADS)

    De Novellis, Vincenzo; Castaldo, Raffaele; Solaro, Giuseppe; De Luca, Claudio; Pepe, Susi; Bonano, Manuela; Casu, Francesco; Zinno, Ivana; Manunta, Michele; Lanari, Riccardo; Tizzani, Pietro

    2016-04-01

    A Mw 7.8 earthquake struck Nepal on 25 April 2015 at 06:11:26 UTC, killing more than 9,000 people, injuring more than 23,000 and producing extensive damages. The main seismic event, known as the Gorkha earthquake, had its epicenter localized at ~82 km NW of the Kathmandu city and the hypocenter at a depth of approximately 15 km. After the main shock event, about 100 aftershocks occurred during the following months, propagating toward the south-east direction; in particular, the most energetic shocks were the Mw 6.7 and Mw 7.3 occurred on 26 April and 12 May, respectively. In this study, we model the causative fault of the earthquake by jointly exploiting surface deformation retrieved by the DInSAR measurements collected through the Sentinel 1-A (S1A) space-borne sensor and the available geological, structural and seismological information. We first exploit the analytical solution performing a back-analysis of the ground deformation detected by the first co-seismic S1A interferogram, computed by exploiting the 17/04/2015 and 29/04/2015 SAR acquisitions and encompassing the main earthquake and some aftershocks, to search for the location and geometry of the fault plane. Starting from these findings and by benefiting from the available geological, structural and seismological data, we carry out a Finite Element (FE)-based 2D modelling of the causative fault, in order to evaluate the impact of the geological structures activated during the seismic event on the distribution of the ground deformation field. The obtained results show that the causative fault has a rather complex compressive structure, dipping northward, formed by segments with different dip angles: 6° the deep segment and 60° the shallower one. Therefore, although the hypocenters of the main shock and most of the more energetic aftershocks are located along the deeper plane, corresponding to a segment of the Main Himalayan Thrust (MHT), the FE solution also indicates the contribution of the shallower

  19. Investigating a damaging buried sinkhole cluster in an urban area (Zaragoza city, NE Spain) integrating multiple techniques: Geomorphological surveys, DInSAR, DEMs, GPR, ERT, and trenching

    NASA Astrophysics Data System (ADS)

    Carbonel, Domingo; Rodríguez-Tribaldos, Verónica; Gutiérrez, Francisco; Galve, Jorge Pedro; Guerrero, Jesús; Zarroca, Mario; Roqué, Carles; Linares, Rogelio; McCalpin, James P.; Acosta, Enrique

    2015-01-01

    This contribution analyses a complex sinkhole cluster buried by urban elements in the mantled evaporite karst of Zaragoza city, NE Spain, where active subsidence has caused significant economic losses (~ 0.3 million Euro). The investigation, conducted after the development of the area, has involved the application of multiple surface and subsurface techniques. A detailed map of modern surface deformation indicates two active coalescing sinkholes, whereas the interpretation of old aerial photographs reveals the presence of two additional dormant sinkholes beneath human structures that might reactivate in the near future. DInSAR (Differential Interferometry Synthetic Aperture Radar) displacement data have limited spatial coverage mainly due to high subsidence rates and surface changes (re-pavement), and the Electrical Resistivity Tomography (ERT) and trenching investigations were severely restricted by the presence of urban elements. Nonetheless, the three techniques consistently indicate that the area affected by subsidence is larger than that defined by surface deformation features. The performance of the Ground Penetrating Radar (GPR) technique was adversely affected by the presence of highly conductive and massive anthropogenic deposits, but some profiles reveal that subsidence in the central sector of one of the sinkholes is mainly accommodated by sagging. The stratigraphic and structural relationships observed in a trench dug across the topographic margin of one of the sinkholes may be alternatively interpreted by three collapse events of around 0.6 m that occurred after 290 yr BP, or by progressive fault displacement combined with episodic anthropogenic excavation and fill. Average subsidence rates of > 6.6 mm/yr and 40 mm/yr have been calculated using stratigraphic markers dated by the radiocarbon method and historical information, respectively. This case study illustrates the need of conducting thorough investigations in sinkhole areas during the pre

  20. Determination of absorbed dose to water in reference conditions for radiotherapy kilovoltage x-rays between 10 and 300 kV: a comparison of the data in the IAEA, IPEMB, DIN and NCS dosimetry protocols

    NASA Astrophysics Data System (ADS)

    Peixoto, J. G. P.; Andreo, P.

    2000-03-01

    A comparison of four of the most commonly used dosimetry protocols for the determination of absorbed dose to water in therapeutic kilovoltage x-rays using an ionization chamber (IAEA TRS-277, IPEMB, DIN and NCS) has been carried out. Owing to the different energy ranges and HVLs recommended by each protocol, backscatter factors, water-to-air mass energy absorption coefficient ratios and perturbation correction factors have been recast to a common quality range that all protocols satisfy individually to make a comparison possible. The results of the comparison show that in the sometimes reduced quality range originally included by the different protocols, determinations of absorbed dose to water at all beam qualities agree to within ±1.0% with that obtained using the second edition of the IAEA TRS-277 code of practice (1997). The extrapolation of data to a common beam quality range practically preserves the agreement for all the protocols except for that issued by the NCS at the extremes of the range, where differences of up to 1.8% and 1.4% have been found for low and medium energies respectively. In all cases the DIN protocol yields very good agreement with TRS-277.

  1. Spatio-temporal evolution of anthropogenic deformation around Cerro Prieto geothermal field in the Mexicali Valley, B.C., Mexico, between 1993 and 2009 from DInSAR and leveling.

    NASA Astrophysics Data System (ADS)

    Sarychikhina, Olga; Glowacka, Ewa; Robles, Braulio; Mojarro, Jose

    2014-05-01

    Land subsidence is an environmental hazard which could be caused by withdrawal of large amounts of fluid from beneath the earth's surface. Land subsidence is an issue in several geothermal fields worldwide (e.g., Geysers, USA (Mossop and Segall, 1997), Wairakei-Tauhara, New Zealand (Allis et al., 2009)). Cerro Prieto geothermal field (CPGF), located in the Mexicali Valley, northwest Mexico, is the oldest and largest Mexican geothermal field in operation and has been producing electricity since 1973. The large amount of geothermal fluids extracted to supply steam to the power plants has resulted in considerable deformation in and around the field (e.g. Glowacka et al., 1996, 1999; Carnec and Fabriol, 1999; Sarychikhina et al., 2011). The deformation includes land subsidence and related ground fissuring and faulting. These phenomena have produced severe damages to the local infrastructure such as roads, irrigation canals and other facilities. Detection of land subsidence and monitoring of the spatial and temporal changes of its pattern and magnitude can provide important information about the dynamics of this process and controlling geological structures. The technique of Differential Synthetic Aperture Radar Interferometry (DInSAR) has been demonstrated to be a very effective technique for measuring ground deformation. This study presents an application of DInSAR interferogram stacking technique to investigate the land subsidence in the Mexicali Valley near CPGF. C-band ENVISAR ASAR images acquired between 2003 and 2009 from the ascending (track 306, frame 639) and descending track (track 84, frame 2961), obtained from the European Space Agency (ESA), as part of ESA CAT-1 project (ID - C1P3508), were used. Gamma ISP and DIFF/GEO software packages were used to calculate differential interferograms from SLC data and for differential interferograms stacking (Wegmüller and Werner, 1997). Eight average annual deformation rate maps were generated for 2005 (descending

  2. (1)H, (13)C, and (15)N backbone resonance assignments of the full-length 40 kDa S. acidocaldarius Y-family DNA polymerase, dinB homolog.

    PubMed

    Moro, Sean L; Cocco, Melanie J

    2015-10-01

    The dinB homolog (Dbh) is a member of the Y-family of translesion DNA polymerases, which are specialized to accurately replicate DNA across from a wide variety of lesions in living cells. Lesioned bases block the progression of high-fidelity polymerases and cause detrimental replication fork stalling; Y-family polymerases can bypass these lesions. The active site of the translesion synthesis polymerase is more open than that of a replicative polymerase; consequently Dbh polymerizes with low fidelity. Bypass polymerases also have low processivity. Short extension past the lesion allows the high-fidelity polymerase to switch back onto the site of replication. Dbh and the other Y-family polymerases have been used as structural models to investigate the mechanisms of DNA polymerization and lesion bypass. Many high-resolution crystal structures of Y-family polymerases have been reported. NMR dynamics studies can complement these structures by providing a measure of protein motions. Here we report the (15)N, (1)H, and (13)C backbone resonance assignments at two temperatures (35 and 50 °C) for Sulfolobus acidocaldarius Dbh polymerase. Backbone resonance assignments have been obtained for 86 % of the residues. The polymerase active site is assigned as well as the majority of residues in each of the four domains. PMID:26154586

  3. Dinámica de pulsares jóvenes

    NASA Astrophysics Data System (ADS)

    Allen, M. P.; Horvath, J. E.

    La observación detallada de los pulsos en varios pulsares jóvenes (τ < 105 yr) permitió detectar varios aumentos en la frecuencia de emisión gliches con cambios permanentes en Δ {˙ Ω} / {˙ Ω}. A través de las ecuaciones de movimiento se investiga, utilizando parametrizaciones simples, la hipótesis de que el ángulo entre Ω y el dipolo magnético M sea responsable de ese comportamiento. Se derivan curvas analíticas para la evolución de Ω (t) y se estudia la posibilidad de describir un conjunto de observables (incluyendo el braking index n) con estos modelos.

  4. Determining the role of hydrologic variability on DIN export from the Catskill Mountains

    EPA Science Inventory

    The Catskill region of New York State receives some of the highest rates of atmospheric nitrogen deposition in the northeastern US (approximately 6.2 kg ha-1 yr-1), and headwater streams have elevated nitrate concentrations (mean of stream surveys = 0.27 – 0.35 mg N l-1). Althou...

  5. Evidence for a non-opioid sigma binding site din the guinea-pig myenteric plexus

    SciTech Connect

    Roman, F.; Pascaud, X.; Vauche, D.; Junien, J.

    1988-01-01

    The presence of a binding site to (+)-(/sup 3/H)SKF 10,047 was demonstrated in a guinea-pig myenteric plexus (MYP) membrane preparation. Specific binding to this receptor was saturable, reversible, linear with protein concentration and consisted of two components, a high affinity site and a low affinity site. Morphine and naloxone 10/sup -4/M were unable to displace (+)-(/sup 3/H)SKF 10,047 binding. Haloperidol, imipramine, ethylketocyclazocine and propranolol were among the most potent compounds to inhibit this specific binding. These results suggest the presence of a non-opioid haloperidol sensitive sigma receptor in the MYP of the guinea-pig.

  6. Estudio de eventos dinámicos en ondas milimétricas

    NASA Astrophysics Data System (ADS)

    Cristiani, G.; Martínez, G.; Raulin, J. P.; Giménez de Castro, G.; Rovira, M.

    El estudio de la actividad solar con el telescopio para ondas submilimétricas (SST), nos permite, por vez primera, trabajar con una resolución temporal de milisegundos. Este hecho ha permitido observar emisión nunca antes comprobada, pulsos o flashes extremadamente rápidos provenientes de las regiones activas, los cuales parecen preanunciar fulguraciones en dichas regiones. Se muestra, como ejemplo, una fulguración observada con el telescopio HASTA en Hα, y los pulsos rápidos que la preceden observados con el SST. Para cuantificar la influencia de la atmósfera terrestre en los datos registrados, se está estudiando la variabilidad temporal de las opacidades a las frecuecias de trabajo (212 y 405 Ghz), es decir los rangos de tiempo para los cuales la opacidad puede variar apreciablemente. Con este fin se calcula la eficiencia de cada canal multiplicada por la temperatura del Sol a estas frecuencias (estas son conocidas con una incerteza del 20 %). Se muestran los resultados obtenidos para cada canal, en donde se grafica este producto como función de la opacidad.

  7. Subsidence activity maps derived from DInSAR data: Orihuela case study

    NASA Astrophysics Data System (ADS)

    Sanabria, M. P.; Guardiola-Albert, C.; Tomás, R.; Herrera, G.; Prieto, A.; Sánchez, H.; Tessitore, S.

    2014-05-01

    A new methodology is proposed to produce subsidence activity maps based on the geostatistical analysis of persistent scatterer interferometry (PSI) data. PSI displacement measurements are interpolated based on conditional Sequential Gaussian Simulation (SGS) to calculate multiple equiprobable realizations of subsidence. The result from this process is a series of interpolated subsidence values, with an estimation of the spatial variability and a confidence level on the interpolation. These maps complement the PSI displacement map, improving the identification of wide subsiding areas at a regional scale. At a local scale, they can be used to identify buildings susceptible to suffer subsidence related damages. In order to do so, it is necessary to calculate the maximum differential settlement and the maximum angular distortion for each building of the study area. Based on PSI-derived parameters those buildings in which the serviceability limit state has been exceeded, and where in situ forensic analysis should be made, can be automatically identified. This methodology has been tested in the city of Orihuela (SE Spain) for the study of historical buildings damaged during the last two decades by subsidence due to aquifer overexploitation. The qualitative evaluation of the results from the methodology carried out in buildings where damages have been reported shows a success rate of 100%.

  8. Copiii Din Romania Despre Drepturile Omului (Romanian Children about Human Rights). Children's Album.

    ERIC Educational Resources Information Center

    Georgescu, Dakmara

    This album addresses how civic education should be taught in Romanian schools. Civic education, like any other form of education, must be gradual and adapted to the specifics of the respective age. The album can be used by any person involved in developing future citizens. The document includes drawings and texts made by students who were apart of…

  9. Sediment DIN fluxes and preferential recycling of benthic microalgal nitrogen in a shallow macrotidal estuary

    USGS Publications Warehouse

    Tobias, C.; Giblin, A.; McClelland, J.; Tucker, J.; Peterson, B.

    2003-01-01

    Sediment-water fluxes of NH4+, NO3-, dissolved inorganic carbon, and O2 were measured in cores collected from the upper Rowley River estuary, Massachusetts, and used to calculate rates of organic nitrogen (N) mineralization, nitrification, and coupled and direct denitrification (DNF). The cores contained 15N label in benthic microalgae (BMA) and in NO3- in the overlying water as a result of an ongoing whole-estuary 15NO 3- enrichment study (NISOTREX II). The tracer allowed for estimation of gross NO3- regeneration in sediments and the contribution of BMA derived N to total mineralization. The mean mineralization rate between sites was 16.0 ?? 2.0 mmol N m-2 d-1. Approximately 13 to 56% of the mineralized N was nitrified at rates ranging from 1.8 to 10.1 mmol N m-2 d-1. Total denitrification was dominated by direct DNF (3.6 mmol N m-2 d -1) furthest upstream, where NO3- concentrations were highest. Coupled DNF was most important (8.0 mmol N m -2 d-1) in the sediments with high nitrification and low water column NO3-. A gross NO3- flux from sediments to water of 0.9 to 2.1 mmol N m-2 d-1 was estimated from the isotope dilution of ??15NO 3- in the overlying water of the cores. The isotope dilution seen in the cores was also detected as a deviation from conservative ??15NO3- mixing along estuarine transects. Incorporation of this NO3- regeneration into the DNF calculations effectively increased the estimate of direct DNF by up to 50% and decreased the coupled DNF estimate by up to 220%. Increasing ?? 15NH4+ in the water of the cores indicated that the 15N-labelled BMA were preferentially mineralized over bulk sediment organic N. Additional 15N enrichments in the sediment bacterial biomarker diaminopimelic acid showed a link among 15N-labeled BMA, active bacteria, and 15NH 4+released to the overlying water. Based on ?? 15NH4+ enrichments in the cores, BMA accounted for approximately 50 to 100% of the N mineralized. An isotopic enrichment of ??15NH4+ above background in the estuary was observed at a magnitude consistent with the core-based rates of BMA mineralization. These results provide further evidence that BMA are not unidirectional sinks for water column-dissolved organic nitrogen, but instead act to turn over N between sediments and estuarine water on the scale of days.

  10. The DinJ/RelE toxin-antitoxin system suppresses virulence in Xylella fastidiosa

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Xylella fastidiosa, the causal agent of a number agriculturally important plant diseases, encodes multiple toxin-antitoxin (TA) systems. TA modules consist of a toxin protein co-expressed with a specific antitoxin, and are often acquired through horizontal gene transfer. Antitoxin molecules (RNA or ...

  11. Comportamiento dinámico de asteroides en la resonancia 2:1

    NASA Astrophysics Data System (ADS)

    López García, F.; Brunini, A.

    El propósito de este trabajo es mostrar los resultados obtenidos del estudio del movimiento de asteroides cercanos a la conmensurabilidad 2:1 con el movimiento medio de Júpiter, analizando el comportamiento de órbitas de asteroides reales y ficticios. Se analizan los tiempos de Lyapunov obtenidos a partir de la integración de las ecuaciones del movimiento. Se han estudiado la órbitas utilizando el modelo de cinco cuerpos y se han considerado los casos planar y espacial. Las simulaciones numéricas se realizaron utilizando integradores simpléticos y el intervalo de tiempo de integración fue de T = 107 años.

  12. 77 FR 18752 - Benzidine-Based Chemical Substances; Di-n

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-28

    ...Under the Toxic Substances Control Act (TSCA), EPA is proposing: To add nine benzidine-based chemical substances to the Significant New Use Rule (SNUR) on benzidine-based chemical substances; a SNUR for di-n-pentyl phthalate (DnPP) (1,2-benzenedicarboxylic acid, 1,2-dipentyl ester) (CAS No. 131-18-0); and a SNUR for alkanes, C12-13, chloro (CAS No. 71011-12-6). In the case of the......

  13. Monitoring of infrastructural sites by means of advanced multi-temporal DInSAR methods

    NASA Astrophysics Data System (ADS)

    Vollrath, Andreas; Zucca, Francesco; Stramondo, Salvatore

    2013-10-01

    With the launch of Sentinel-1, advanced interferometric measurements will become more applicable then ever. The foreseen standard Wide Area Product (WAP), with its higher spatial and temporal resolution than comparable SAR missions, will provide the basement for the use of new wide scale and multitemporal analysis. By now the use of SAR interferometry methods with respect to risk assessment are mainly conducted for active tectonic zones, plate boundaries, volcanoes as well as urban areas, where local surface movement rates exceed the expected error and enough pixels per area contain a relatively stable phase. This study, in contrast, aims to focus on infrastructural sites that are located outside cities and are therefore surrounded by rural landscapes. The stumbling bock was given by the communication letter by the European Commission with regard to the stress tests of nuclear power plants in Europe in 2012. It is mentioned that continuously re-evaluated risk and safety assessments are necessary to guarantee highest possible security to the European citizens and environment. This is also true for other infrastructural sites, that are prone to diverse geophysical hazards. In combination with GPS and broadband seismology, multitemporal Differential Interferometric SAR approaches demonstrated great potential in contributing valuable information to surface movement phenomenas. At this stage of the project, first results of the Stamps-MTI approach (combined PSInSAR and SBAS) will be presented for the industrial area around Priolo Gargallo in South East Sicily by using ENVISAT ASAR IM mode data from 2003-2010. This area is located between the Malta Escarpment fault system and the Hyblean plateau and is prone to earthquake and tsunami risk. It features a high density of oil refineries that are directly located at the coast. The general potential of these techniques with respect to the SENTINEL-1 mission will be shown for this area and a road-map for further improvements is given in order to overcome limitations that refer to the influence of the atmosphere, orbit or DEM errors. Further steps will also include validation and tectonic modeling for risk assessment.

  14. [Certification according to DIN EN ISO 9001 in MDK in Hessia--a report of experiences].

    PubMed

    Hübner, M; Andersen, J; von Mittelstaedt, G

    2000-02-01

    Quality assurance is a routine and indispensable part of the work of the Medizinische Dienst der Krankenversicherung (MDK) (Medical Services of statutory health insurance bodies in Germany). To optimize the internal organisational processes, a quality assurance system (QAS), certified by EQ Zert, Ulm (an accredited certifier of the TGA), was implemented for the first time in one of the departments of the MDK, namely, the hospital department. Employees and management consider the advantages to be as follows: the aims of the department are clearly described. the processes are now self-evident and more strictly observed, overlaps to other fields of the company are defined. Both an Error Management System with follow up measures, and a Project Management System to control projects have been implemented. The documentation system was standardized, and consequently relocating documents has become more streamlined. An MDK self-evaluation was performed as part of an internal audit. The audit showed that the QAS of the hospital department of the MDK in Hesse is indeed highly developed. It is anticipated that gradually other fields of the MDK will also be certified, to comply with the changing requirements. PMID:10740360

  15. Nasir al-Din al-Tusi's Memoir on Astronomy (al-Tadhkira fi cilm al-hay'a)

    NASA Astrophysics Data System (ADS)

    Ragep, F. J.

    Tusi (1201-1274 a.d.) was an Arabic scholar whose writings became the standard texts in several disciplines for several centuries. They include editions of Euclid's Elements and Ptolemy's Almagest, as well as other books on mathematics and astronomy, and books on logic, ethics, religion. He wrote the Tadhkira, to be "a summary account of `astronomy' presented in narrative form. The details are expounded and proofs of the validity are furnished in the Almagest. Indeed, ours would not be a complete science if taken in isolation from the Almagest for it is a report of what is established therein." It is thus not only a sort of running commentary on the Almagest, but also an account of medieval Arabic cosmology. Jamil Ragep has not only translated the text of the Tadhkira, but has also provided a lengthy introduction with a biography of Tusi and a discussion of the context in which the Tadhkira was written and its influence, as well as a detailed and thorough commentary on each section of the text.

  16. Linear and Non-Linear Long-Term Terrain Deformation with DInSAR (CPT: Coherent Pixels Technique)

    NASA Astrophysics Data System (ADS)

    Mallorqui, J. J.; Mora, O.; Blanco, P.; Broquetas, A.

    2004-06-01

    / In this paper, an advanced technique for the generation of deformation maps using SAR (Synthetic Aperture Radar) data is presented. The algorithm, called the Coherent Pixels Technique, estimates the linear and non-linear components of the displacement, the error of the Digital Elevation Model (DEM) used to cancel the topographic terms, and the atmospheric artifacts from a reduced set of low spatial resolution interferograms. The pixel candidates are selected from those presenting a good coherence level in the whole set of interferograms and the resulting non-uniform mesh tessellated with the Delauney triangulation to establish connections among them. The linear component of movement and DEM error are estimated adjusting a linear model to the data only on the connections. Later on, this information, once integrated to retrieve the absolute values, is used to calculate the non-linear component of movement and atmospheric artifacts with alternate filtering techniques in both temporal and spatial domains. The method presents high flexibility with respect to the required number of images and the baselines length. However, better results are obtained with large datasets of short baseline interferograms. The technique has been tested with ERS SAR data from an area of Catalonia (Spain) and the Campi Flegrei area (Naples, Italy) and validated with on-field precise levelling measurements.

  17. Fricción dinámica: Influencia de órbitas caóticas

    NASA Astrophysics Data System (ADS)

    Cora, S. A.; Vergne, M. M.; Muzzio, J. C.

    We have used numerical experiments to investigate the orbital decay, caused by dynamical friction, of a rigid satellite which moves within a larger stellar system (a galaxy), whose potential is non-integrable. This type of potential has regular and irregular orbits, being the last ones chaotic respect of having exponential sensitivity to perturbations, a feature that makes them to relax in a time much shorter than regular ones. Assuming that the dynamical friction is inversely proportional to the relaxation time, this phenomenon would increase in the presence of chaotic orbits (Pfenniger 1986). Therefore, we present preliminary numerical results to check this idea. We considered two different triaxial galaxy models with different percentage of chaotic orbits. 1) The triaxial generalization of spherical models of Dehnen (1993), with a constant-density core, where the orbits can be identified with one of the four families of regular orbits in Stackel potentials (boxes and the three families of tubes). When a central compact object (black hole) is included a porcentage of the boxlike orbits are rendered chaotic. 2) The modified Satoh galaxy model (Satoh 1980) without rotation, in which the orbits are mostly regular (boxes and tubes). However, if we consider the triaxial galaxy with rotation the resulting potential is non-integrable with a high degree of chaos. The satellite is modelled by a softened point mass, moving on a circular orbit in the spherical model. To integrate he equation of motion a non-self-consistent code is used. The results of a few simulations suggest that the presence of chaotic orbits does not affect significantly the orbital decay of the satellite.

  18. Preliminary results of ESA Category-1 Project 5834 "Application of DInSAR technique to areas of active ground deformations"

    NASA Astrophysics Data System (ADS)

    Massa, B.; D'Auria, L.

    2009-04-01

    We have established a processing chain of Synthetic Aperture Radar (SAR) data for identification and parametrisation of deformation sources in areas of active ground deformation (e.g. seismogenic areas, volcanic districts). SAR data from European Space Agency (ESA) satellites ERS-2 and ENVISAT are used. SAR and InSAR data processing LEVEL 0 SAR data are focussed to Single Look Complex (SLC) through ROI_PAC (Copyright 2002-2008, Caltech/Jet Propulsion Laboratory). We perform an advanced data processing using Doris (Kampes and Usai, 1999) a single program that can do most common steps of the interferometric radar processing starting from SLC data to generation of interferometric products and geocoding. Unwrapping of interferometric phase is performed using the public domain software snaphu (Chen and Zebker, 2001). Modeling of deformation sources We propose a novel inversion approach base on non-linear inversion. The forward modeling is provided by the semi-analytic deformation model for point sources and finite faults. The parameters of the fault (center position, width, height, rake and seismic moment) are inverted using a combination of non-linear optimization algorithms (as Monte-Carlo, Nelder&Mead Simplex and Simulated Annealing). The misfit function defined for the optimization is based on the L2 norm of the error weighted by the coherence of the considered spatial point. Test datasets To test our modeling procedure we chose three different study areas, refer to mainly strike-slip seismogenic sources with different orientation to respect satellite Line Of Sight (LOS): December 26 2003 Iranian earthquake (Bam e.), data from both ascending and descending passes of ENVISAT ASAR narrow swath IS2 (RAW and SLCs); August 17 1999 Turkey earthquake (Izmit e.), data from both ascending and descending passes of ERS-2 AMI SAR (SLCs); June 17-21 2000 Iceland earthquakes, data from both ascending and descending passes of ERS-2 AMI SAR (SLCs). Tests carried over real deformation unwrapped interferograms have shown encouraging results. Preliminary modeling of deformation sources is presented. Acknowledgements Data provided by the European Space Agency (ESA CAT-1 Project 5834, P.I. B. Massa). References Chen W., H. Zebker A. (2001) -Two-dimensional phase unwrapping with use of statistical models for cost functions in nonlinear optimization- Journal of the Optical Society of America, 18, 338-351. Kampes B., Usai S. (1999) -Doris: The Delft object-oriented Radar Interferometric software.- In: proceedings ITC 2nd ORS symposium, August 1999 (CD-ROM).

  19. Extracting a Whisper from the DIN: A Bayesian-Inductive Approach to Learning an Anticipatory Model of Cavitation

    SciTech Connect

    Kercel, S.W.

    1999-11-07

    For several reasons, Bayesian parameter estimation is superior to other methods for inductively learning a model for an anticipatory system. Since it exploits prior knowledge, the analysis begins from a more advantageous starting point than other methods. Also, since "nuisance parameters" can be removed from the Bayesian analysis, the description of the model need not be as complete as is necessary for such methods as matched filtering. In the limit of perfectly random noise and a perfect description of the model, the signal-to-noise ratio improves as the square root of the number of samples in the data. Even with the imperfections of real-world data, Bayesian methods approach this ideal limit of performance more closely than other methods. These capabilities provide a strategy for addressing a major unsolved problem in pump operation: the identification of precursors of cavitation. Cavitation causes immediate degradation of pump performance and ultimate destruction of the pump. However, the most efficient point to operate a pump is just below the threshold of cavitation. It might be hoped that a straightforward method to minimize pump cavitation damage would be to simply adjust the operating point until the inception of cavitation is detected and then to slightly readjust the operating point to let the cavitation vanish. However, due to the continuously evolving state of the fluid moving through the pump, the threshold of cavitation tends to wander. What is needed is to anticipate cavitation, and this requires the detection and identification of precursor features that occur just before cavitation starts.

  20. Avoiding a Din at Dinner or, Teaching Students to Argue for Themselves: Year 13 Plan a Historians' Dinner Party

    ERIC Educational Resources Information Center

    Richards, Keeley

    2012-01-01

    Keeley Richards secured a fundamental shift in some of her Year 13 students' ability to argue. She did it by getting them to engage more fully with the practice of argument itself, as enacted by four historians. At the centre of her lesson sequence was an original activity: the historians' dinner party. Richards uses this activity to point to…

  1. Liquid metal embrittlement susceptibility of welded MANET II (DIN 1.4914) in liquid Pb17Li.

    NASA Astrophysics Data System (ADS)

    Sample, T.; Fenici, P.; Kolbe, H.

    1996-10-01

    TIG welding of 12 mm thick plates of MANET II steel was accomplished using filler material of the same composition. Tensile tests, at a constant displacement rate of 0.1 mm min -1 (corresponding to an initial strain rate of 1.1 × 10 -4 s -1), were carried out on specimens consisting solely of the weld, HAZ and bulk material under vacuum and liquid Pb17Li at 250 and 400°C. The post-weld heat treatment (750°C/4 h air cooled) which was given to the welded plates was sufficient to prevent any liquid metal embrittlement. The presence of oxide inclusions and pores in the weld reduced the extent of plastic strain at rupture exhibited by some of the welds.

  2. Pump testing strategies and associated tribological considerations -- Vane pump testing methods ASTM D 2882, IP281, and DIN 51389

    SciTech Connect

    Reichel, J.

    1997-12-31

    Various test methods have been developed to determine the performance limits of various classes of hydraulic fluids. Lubrication capacity depends on various fundamental fluid parameters including viscosity and anti-wear properties. Critical elements of hydraulic pump and motor lubrication, which is characterized by sliding line-contact wear, will be discussed here. In vane pumps, the pressure loaded tips of the vanes are under Hertz-type load in contact with the surface of the cam ring, and rotate at a high speed creating a sliding line-contact. Due to this sliding line contact, the vane pump is the best-suited instrument for determination of the anti-wear performance of hydraulic fluids within acceptable time and at reasonable expense. Alternatively, hydraulic pump and motor testing may require greater energy, 150 kW or more over a period of more than 1,000 hours significantly increasing the cost of testing. Furthermore, tests on smaller versions of one type of pump or motor do not necessarily correlate with larger units of similar design. Therefore, it would be desirable to develop a laboratory test that: utilizes a rig with standard wear parts, provides a selective method for identifying various forms of lubricant failure, and that permits tests correlation with a wide variety of hydraulic pumps and motors used in the industry today.

  3. Non-invasive deformation analysis of historical buildings through the advanced SBAS-DInSAR technique: the case of the city of Roma (Italy)

    NASA Astrophysics Data System (ADS)

    Manunta, Michele; Bonano, Manuela; Marsella, Maria; Lanari, Riccardo

    2010-05-01

    The monitoring of urban areas and man-made structures is of key importance for the preservation of artistic, archaeological and architectural heritage. In this context, the remote sensing techniques may allow non-invasive analysis of large areas by exploiting long time series of satellite data. Among these techniques, the Synthetic Aperture Radar (SAR) Interferometry (InSAR) has already demonstrated to be an effective tool for monitoring the displacements occurring in the historical and artistic heritage located in the historical city centers. As a matter of fact, the InSAR technique allows producing spatially dense deformation maps with centimeter to millimeter accuracy, by exploiting the phase difference (interferogram) of temporally separated SAR images relevant to the same analyzed area. In order to guarantee the monitoring of urban area displacements, it is strategic to provide very long term deformation time series by also exploiting SAR data acquired by different sensors. Accordingly, ERS/ENVISAT data archive, providing acquisitions spanning the 1992-2010 time period, might allow us generating very long term deformation time-series. However, an ERS/ENVISAT data combination is limited by the two sensors slightly different carrier frequencies: 5.331 GHz for the ENVISAT sensor and 5.3 GHz for the ERS one. Therefore, because the interferometric phase is dependent on the radiation wavelength, the generation of conventional ERS/ENVISAT cross-interferograms is strongly affected by the induced decorrelation effects. In this work we show the effectiveness of the Small BAseline Subset (SBAS) (Berardino et al., 2002; Lanari et al., 2004) approach for the conservation, monitoring and risk prevention of cultural heritage. Indeed, the SBAS technique allows us to produce deformation time series at the scale of the single building by processing very long sequences of ERS-1/2 and ENVISAT (IS2 swath) SAR data, acquired with the same illumination geometry. In particular, the SBAS approach exploits only conventional ERS/ERS and ENVISAT/ENVISAT differential interferograms, in order to limit the decorrelation effects and improve the number of the detected coherent points. Accordingly, this method may play a key role for supporting the study and conservation strategies of the historical built heritage, monuments and artistic artifacts, due to its capability of generating deformation time series spanning time interval of more than 15 years. The presented results, achieved by applying the full resolution SBAS approach to an ERS/ENVISAT dataset relevant to the city of Rome, demonstrate the effectiveness of this technique to detect and analyze the temporal evolution of possible deformation phenomena affecting historical buildings within the investigated 1992-2009 time period. [1] P. Berardino, G. Fornaro, R. Lanari, and E. Sansosti, "A new Algorithm for Surface Deformation Monitoring based on Small Baseline Differential SAR Interferograms", IEEE Trans.Geosci. Remote Sens., Vol. 40, No 11, pp. 2375-2383, 2002. [2] Lanari, R., Mora, O., Manunta, M., Mallorquí, J.J., Berardino, P. and Sansosti, E., "A small baseline approach for investigating deformations on full resolution differential SAR interferograms", IEEE Transactions on Geoscience and Remote Sensing, Vol. 42, pp. 1377-1386, 2004.

  4. EVALUATION OF IODINE BASED IMPINGER SOLUTIONS FOR THE EFFICIENT CAPTURE OF HG USING DIRECT INJECTION NEBULIZATION INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (DIN-ICP/MS) ANALYSIS

    EPA Science Inventory

    Currently there are no EPA reference sampling methods that have been promulgated for measuring stack emissions of Hg from coal combustion sources, however, EPA Method 29 is most commonly applied. The draft ASTM Ontario Hydro Method for measuring oxidized, elemental, particulate-b...

  5. Ground Motion Measurement in the Lake Mead Area (Nevada, USA), by DinSAR Time Series Analysis : Probing of the Lithosphere Rheological Structure

    NASA Astrophysics Data System (ADS)

    Doin, M.; Cavalié, O.; Laserre, C.; Briole, P.

    2006-12-01

    SAR interferometry has proven to be a reliable method for detecting small displacements due to ground subsidence. In this study, we measure ground motion around the lake Mead (Nevada, USA) using InSAR. This artificial lake has been filled with water in 1935. An earlier study, based on leveling measurements, has shown that the load associated with lake impoundement induced a subsidence of 17 centimeters. This relaxation process has been argued as analogous to the postglacial rebound, but at a smaller spatial scale and with a much lower viscous relaxation scale. To quantify the deformation and thus constrain the crust and mantle rheological parameters in the lake area, we analyze multiple interferograms (241) based on 43 ERS images acquired between 1992 and 2001. With baselines smaller than 300 m, all interferograms have a very good coherence due to the desert region. Most of interferograms show strong atmospheric artefacts that are partly due to the variation of water vapor vertical stratification between two satellite passes. This tropospheric delay is computed for each interferogram and then inverted for each date of SAR images before interferogram correction. These corrections are validated using data from global atmospheric models (ERA40). Corrected interferograms are then inverted to solve for the time series of ground motion in the lake Mead area. The linear inversion treats each pixel independently from its neighbours and uses the data redundancy to reduce errors such as local decorrelations. Additionnal constraints such as temporal smoothing allow to reduce the local atmospheric artefacts. We obtain a time series of the deformation in the lake Mead area with a millimetric accuracy. The deformation is non linear in time and spreads over a large spatial scale. In particular, we observe a subsidence of up to 16 mm between 1995 and 1998 due to a 10 meters water level increase, followed by an uplift due to the drop of the water level after 2000. The deformation model allows to constrain the rheology in the lake Mead area. The model takes into account the loading history of the lake since 1935. We show that a simple elastic response with parameters constrained by seismic wave propagation does not explain the amplitude and spatial wavelength of the observed motion. To fit the data, a low viscosity (around 1018 Pa.s) in the lithospheric mantle below a 30 km thick elastic layer is required.

  6. Measurement of elemental speciation by liquid chromatography -- inductively coupled plasma mass spectrometry (LC-ICP-MS) with the direct injection nebulizer (DIN)

    SciTech Connect

    Shum, S.

    1993-05-01

    This thesis is divided into 4 parts: elemental speciation, speciation of mercury and lead compounds by microbore column LC-ICP-MS with direct injection nebulization, spatially resolved measurements of size and velocity distributions of aerosol droplets from a direct injection nebulizer, and elemental speciation by anion exchange and size exclusion chromatography with detection by ICP-MS with direct injection nebulization. Tabs, figs, refs.

  7. IMPINGER SOLUTIONS FOR THE EFFICIENT CAPTURE OF GASEOUS MERCURY SPECIES USING DIRECT INJECTION NEBULIZATION INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (DIN-ICP/MS) ANALYSIS

    EPA Science Inventory

    Currently there are no EPA reference sampling mehtods that have been promulgated for measuring Hg from coal combustion sources. EPA Method 29 is most commonly applied. The ASTM Ontario Hydro Draft Method for measuring oxidized, elemental, particulate-bound and total Hg is now und...

  8. Interacción de resonancias y transición al caos en un modelo dinámico simple

    NASA Astrophysics Data System (ADS)

    Mestre, M.; Cincotta, P.; Giordano, C.

    In this paper we study the transition to global chaos in a perturbed 2D quartic oscillator. We estimate both, analitically and numerically, the intensity of the perturbation required to have the majority of the phase space dominated by a chaotic dynamics. FULL TEXT IN SPANISH

  9. Advanced DInSAR analysis for building damage assessment in large urban areas: an application to the city of Roma, Italy

    NASA Astrophysics Data System (ADS)

    D'Aranno, Peppe J. V.; Marsella, Maria; Scifoni, Silvia; Scutti, Marianna; Sonnessa, Alberico; Bonano, Manuela

    2015-10-01

    Remote sensing data play an important role for the environmental monitoring because they allow to provide systematic information on very large areas and for a long period of time. Such information must be analyzed, validated and incorporated into proper modeling tools in order to become useful for performing risk assessment analysis. These approaches has been already applied in the field of natural hazard evaluation (i.e. for monitoring seismic, volcanic areas and landslides). However, not enough attention has been devoted to the development of validated methods for implementing quantitative analysis on civil structures. This work is dedicated to the comprehensive utilization of ERS / ENVISAT data store ESA SAR used to detect deformation trends and perform back-analysis of the investigated structures useful to calibrate the damage assessment models. After this preliminary analysis, SAR data of the new satellite mission (ie Cosmo SkyMed) were adopted to monitor the evolution of existent surface deformation processes and to detect new occurrence. The specific objective was to set up a data processing and data analysis chain tailored on a service that sustains the safe maintenance of the built-up environment, including critical construction such as public (schools, hospital, etc), strategic (dam, highways, etc) and also the cultural heritage sites. The analysis of the test area, in the southeastern sector of Roma, has provided three different levels and sub-levels of products from metropolitan area scale (territorial analysis), settlement scale (aggregated analysis) to single structure scale (damage degree associated to the structure).

  10. THE DETERMINATION OF MERCURY AND MULTIPLE METALS IN DIETARY MATRICES USING DIRECT INJECTION NEBULIZATION INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (DIN-ICP/MS)

    EPA Science Inventory

    Mercury (Hg) is a Persistent Bioaccumulative Toxin. Currently, low-level mercury (Hg) and low-level multiple-metals analyses require separate methods. Due to the high costs of performing both types of analyses, research planners often have to choose one or the other. For examp...

  11. Gravity driven and tectonic post-seismic deformation of the April 6 2009 L'Aquila Earthquake detected by Cosmo-SkyMed DInSAR

    NASA Astrophysics Data System (ADS)

    Moro, M.; Albano, M.; Bignami, C.; Malvarosa, F.; Costantini, M.; Saroli, M.; Barba, S.; Falco, S.; Stramondo, S.

    2014-12-01

    The present work focuses on the analysis of post-seismic surface deformation detected in the area of L'Aquila, Central Italy, after the strong earthquake that hit the city and the surrounding villages on April 6th, 2009. The analysis has been carried out thanks to a new dataset of SAR COSMO-SkyMed images covering a time span of 480 days after the mainshock, with the adoption of the Persistent Scatterer Pairs (PSP) approach. This method allows the estimation of surface deformations by exploiting the SAR images at full resolution. In the investigated area two patterns of subsidence have been identified reaching a maximum value of 45 mm in the northeast area of the L'Aquila town. Here the subsidence is mainly ascribable to the post seismic slip release of the Paganica fault and it does not coincide with the maximum measured coseismic subsidence. The time series of the ground deformations also reveal that a large amount of deformation is released in the first three months after the main shock. The second pattern of deformation is centered on the Mt. Ocre ridge, where a detailed photogeological analysis allowed us to identify widespread evidence of morphological elements associated with Deep-seated gravitational slope deformation (DGSD). In particular geomorphologic analyses show evidences of lateral spread DGSD-type features, characterized by the tectonic superimposition of carbonatic sequences and transitional pelagic deposits. In this sector, the observed deformation is ascribable not only to the afterslip of the Paganica fault, but also to a gravitative cause. In order to confirm or reject such hypothesis a 2D numerical finite element models considering two cross sections over the Mt. Ocre ridge has been performed. The coseismic and postseimic deformations have been simulated numerically, considering an elastic-perfectly plastic rheology for the constituent rocks. First results show that most of the postseismic deformation is ascribable to the plastic deformation induced by the gravitative rebalancing of the ridge after the coseismic deformation.

  12. Corrosion resistance of GTAW and EBW welded joints of DIN W. Nr. 1.4462 (UNS S31803): Effect of post-weld-heat-treatment

    SciTech Connect

    Brandi, S.D.; Padilha, A.F.; Wolynec, S.

    1996-12-01

    The duplex stainless steels present better corrosion behavior than the conventional stainless steels. This is due to their chemical composition (Cr, Ni, Mo, N) and a balanced microstructure (50% ferrite). It might be an increase on the volumetric fraction of ferrite, depending on the cooling rate after welding. In the same way the chemical composition can be altered by a loss of the alloying elements during welding, such as N. Both phenomena will decrease the corrosion resistance of the weldment. Autogenous GTAW and EBW were used to join the plates. Several corrosion tests were used to evaluate the behavior of the joints in as-welded (AW) and post-welded-heat-treated (PWHT) conditions. The results were analyzed and correlated to the microstructure of the welded joints. The main conclusion of this work is that corrosion resistance of both joint processes (EBW and GTAW) in as-welded condition is inadequate. PWHT (post-weld-heat-treatment) dissolved the chromium-rich precipitates, restored the equilibrium amount of austenite in the joints and recovered their corrosion resistance.

  13. Diaqua­bis­{5-(pyridin-2-yl-κN)-3-[4-(pyri­din-4-yl)phenyl]-1H-1,2,4-triazol-1-ido-κN 1}zinc

    PubMed Central

    Li, Bin

    2013-01-01

    The asymmetric unit of the title compound, [Zn(C18H12N5)2(H2O)2], consists a ZnII ion, located on an inversion center, a deprotonated 5-pyridin-2-yl-3-[4-(pyridin-4-yl)phen­yl]-1H-1,2,4-triazol-1-ido ligand and a water mol­ecule. The whole mol­ecule is generated by inversion symmetry. The ZnII ion has a distorted octa­hedral coordination geometry, defined by four N atoms from the two deprotonated organic ligands and two water O atoms. In the crystal, O—H⋯N hydrogen bonds link the mol­ecules, forming a three-dimensional network. PMID:23634008

  14. Impact of surface disinfection and sterile draping of furniture on room air quality in a cardiac procedure room with a ventilation and air-conditioning system (extrusion airflow, cleanroom class 1b (DIN 1946-4))

    PubMed Central

    Below, Harald; Ryll, Sylvia; Empen, Klaus; Dornquast, Tina; Felix, Stefan; Rosenau, Heike; Kramer, Sebastian; Kramer, Axel

    2010-01-01

    In a cardiac procedure room, ventilated by a ventilation and air-conditioning system with turbulent mixed airflow, a protection zone in the operating area could be defined through visualization of airflows. Within this protection zone, no turbulence was detectable in the room air. Under the given conditions, disinfection of all surfaces including all furniture and equipment after the last operation and subsequent draping of furniture and all equipment that could not be removed from the room with sterile surgical drapes improved the indoor room air quality from cleanroom class C to cleanroom class B. This also allows procedures with elevated requirements to be performed in room class 1b. PMID:20941336

  15. Estimation of Seismic and Aseismic Deformation in Mexicali Valley, Baja California, Mexico, in the 2006-2009 Period, Using Precise Leveling, DInSAR, Geotechnical Instruments Data, and Modeling

    NASA Astrophysics Data System (ADS)

    Sarychikhina, Olga; Glowacka, Ewa; Robles, Braulio; Nava, F. Alejandro; Guzmán, Miguel

    2015-11-01

    Ground deformation and seismicity in Mexicali Valley, Baja California, Mexico, the southern part of the Mexicali-Imperial valley, are influenced by active tectonics and human activity. In this study, data from two successive leveling surveys in 2006 and 2009/2010 are used to estimate the total deformation occurred in Mexicali Valley during 2006-2009. The leveling data span more than 3.5 years and include deformation from several natural and anthropogenic sources that acted at different temporal and spatial scales during the analyzed period. Because of its large magnitude, the aseismic anthropogenic deformation caused by fluid extraction in the Cerro Prieto geothermal field obscures the deformation caused by other mechanisms and sources. The method of differential interferograms stacking was used to estimate the aseismic (interseismic tectonic and anthropogenic) components of the observed displacement, using SAR images, taken in 2007 during a period when no significant seismicity occurred in the study area. After removing the estimated aseismic signal from the leveling data, residual vertical displacement remained, and to identify possible sources and mechanisms of this displacement, a detailed analysis of records from tiltmeters and creepmeters was performed. The results of this analysis suggest that the residual displacement is mainly caused by moderate-sized seismicity in the area of study. Modeling of the vertical ground deformation caused by the coseismic slip on source fault (primary mechanism) of the two most important earthquakes, May 24, 2006 (Mw = 5.4) and December 30, 2009 (Mw = 5.8), was performed. The modeling results, together with the analysis of geotechnical instruments data, suggests that this moderate-sized seismicity influences the deformation in the study area by coseismic slip on the source fault, triggered slip on secondary faults, and soft sediments deformation.

  16. Effect of Orem Self-Care Program on the Life Quality of Burn Patients Referred to Ghotb-al-Din-e-Shirazi Burn Center, Shiraz, Iran: A Randomized Controlled Trial

    PubMed Central

    Hashemi, Fatemeh; Rahimi Dolatabad, Fatemeh; Yektatalab, Shahrzad; Ayaz, Mehdi; Zare, Najaf; Mansouri, Parisa

    2014-01-01

    Background: Advances in treatment and critical care have largely improved the survival following burns; therefore, the importance of quality of life in burn patients is an issue beyond question. The aim of this study was to determine the effects of Orem self-care program on Quality of Life of burn patients. Methods: A randomized clinical trial study was conducted on 110 eligible burn patients who were selected using easy sampling method and allocated randomly into two groups of experiment and control. The instrument for data collection was a questionnaire, containing demographic and burn information and burn-specific health scale–brief (BSHS-B) questionnaire. For the experiment group, 5 sessions of theoretical training and 75-90 minutes of practical training were accomplished. The quality of life of the patients with burns was assessed in three phases by the BSHS-B questionnaire. The data were analyzed in SPSS-17 using Chi-square test, Fisher’s exact test, Independent t-test and repeated measurement multivariate test. Results: After one month and two months of the use of self-care model, the quality of life of the cases improved from 73.33% to 83.78% and 98.12%, respectively (P<0.001). But the changes in the quality of life of the patients in the control group were not statistically significant (P>0.05). Conclusion: Based on the obtained results of this study, designing and implementing a self-care program based on Orem’s model and the needs of burn patients will improve their quality of life. Therefore, it is recommended that this program should be considered as a part of treatment program for these patients. Trial Registration Number: 2013042112129N1 PMID:25349844

  17. The Collaborative Digital Imaging Network Project

    NASA Astrophysics Data System (ADS)

    Greberman, Melvyn; Goeringer, Fred; Shannon, Roger; Hagen, Raoul; Sweeney, Thomas; Ghaed, Victor; Thomas, Jerry

    1988-06-01

    The Digital Imaging Network (DIN) Project is a collaborative project among numerous components of the Department of Defense, Public Health Service, Veterans Administration, industry, academia, and the MITRE Corporation. The project is evaluating prototype DIN systems (DINS) at Georgetown University (in collaboration with George Washington University) in Washington, DC, and at the University of Washington in Seattle, WA. Results of the project will be used to plan DINS for implementation in fixed and deployable military medical care facilities in the 1990's.

  18. QUANTIFYING SEASONAL SHIFTS IN NITROGEN SOURCES TO OREGON ESTUARIES: PART II: TRANSPORT MODELING

    EPA Science Inventory

    Identifying the sources of dissolved inorganic nitrogen (DIN) in estuaries is complicated by the multiple sources, temporal variability in inputs, and variations in transport. We used a hydrodynamic model to simulate the transport and uptake of three sources of DIN (oceanic, riv...

  19. Quantifying seasonal shifts in nitrogen sources to Oregon estuaries using a transport model combined with stable isotopes

    EPA Science Inventory

    Identifying the sources of dissolved inorganic nitrogen (DIN) in estuaries is complicated by the multiple sources, temporal variability in inputs, and variations in transport. We used a hydrodynamic model to simulate the transport and uptake of three sources of DIN (oceanic, riv...

  20. Factors influencing export of dissolved inorganic nitrogen by major rivers: A new, seasonal, spatially explicit, global model

    EPA Science Inventory

    Substantial effort has focused on understanding spatial variation in dissolved inorganic nitrogen (DIN) export to the coastal zone and specific basins have been studied in depth. Much less is known, however, about seasonal patterns and controls of coastal DIN delivery across larg...

  1. 78 FR 17744 - In the Matter of the Designation of Ansar al-Dine Also Known as Ansar Dine Also Known as Ansar al...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-22

    ... Matter of the Designation of Ansar al-Dine Also Known as Ansar Dine Also Known as Ansar al-Din Also Known as Ancar Dine Also Known as Ansar ul-Din Also Known as Ansar Eddine Also Known as Defenders of the Faith as a Foreign Terrorist Organization Pursuant to Section 219 of the Immigration and Nationality...

  2. 78 FR 17745 - In the Matter of the Designation of Ansar al-Dine, Also Known as Ansar Dine, Also Known as Ansar...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-22

    ... Matter of the Designation of Ansar al-Dine, Also Known as Ansar Dine, Also Known as Ansar al-Din, Also Known as Ancar Dine, Also Known as Ansar ul-Din, Also Known as Ansar Eddine, Also Known as Defenders of the Faith, as a Specially Designated Global Terrorist pursuant to Section 1(b) of Executive,...

  3. 76 FR 33631 - Energy Conservation Program: Test Procedures for Walk-In Coolers and Walk-In Freezers

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ... means the thermal conductivity of a material. * * * * * Panel means a construction component that is not... Sec. 431.304. (2) (c) * * * (2) ASTM C1363-05, (``ASTM C1363''), Standard Test Method for Thermal..., Fax: + 32 2 550 08 19 or http://www.cen.eu/ . (1) DIN EN 13164:2009-02, (``DIN EN 13164''),...

  4. (μ-Acetato-κ2 O:O′)[μ-2,6-bis­({bis­[(pyri­din-2-yl-κN)meth­yl]amino-κN}meth­yl)-4-methyl­phenolato-κ2 O:O](metha­nol-κO)dizinc bis­(perchlorate)

    PubMed Central

    Das, Biswanath; Haukka, Matti; Nordlander, Ebbe

    2014-01-01

    The binuclear title complex, [Zn2(C33H33N6O)(CH3COO2)(CH3OH)](ClO4)2, was synthesized by the reaction between 2,6-bis­({[bis­(pyridin-2-yl)meth­yl]amino}­meth­yl)-4-methyl­phenol (H-BPMP), Zn(OAc)2 and NaClO4. The two ZnII ions are bridged by the phenolate O atom of the octadentate ligand and the acetate group. An additional methanol ligand is terminally coordinated to one of the ZnII ions, rendering the whole structure unsymmetric. Other symmetric dizinc complexes of BPMP have been reported. However, to the best of our knowledge, the present structure, in which the two ZnII ions are distinguishable by the number of coordinating ligands and the coordination geometries (octahedral and square-pyramidal), is unique. The dizinc complex is a dication, and two perchlorate anions balance the charge. The –OH group of the coordinating methanol solvent mol­ecule forms a hydrogen bond with a perchlorate counter-anion. One of the anions is disordered over two sets of sites with an occupancy ratio of 0.734 (2):0.266 (2). PMID:24826088

  5. All the king's horses and 2,4,5-T was the best tool of all; yet 2,4,5-T had a very great fall. All of the facts midst all of the din couldn't put T back together again

    SciTech Connect

    Walstad, J.D.; Dost, F.N.

    1986-09-01

    On January 30 and February 11, 1985, the Environmental Protection Agency (EPA) canceled the use of 2,4,5-T and of silvex, a chemical analog of 2,4,5-T. These actions climaxed the most controversial and turbulent period in the history of forest pest management. Beginning in 1969, stories about 2,4,5-T safety generated intense social activism, litigation, and sometimes violence. Foresters, farmers, ranchers, politicians, manufacturers, environmentalists, scientists, regulatory officials, and local citizens entered the debate. The role of government in pesticide regulation and the integrity of industry and science were being challenged. Although 2,4,5-T and silvex are no longer used, questions remain. Were the ultimate decisions the result of scientific evidence and analysis, or were they a response to public opinion that with all the smoke there must be fire somewhere. Can forestry professionals help the nation avoid controversies of this nature in the future.

  6. The halogen-bonded adduct 1,4-bis(pyri-din-4-yl)buta-1,3-diyne-1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8-hexa-deca-fluoro-1,8-diiodo-octane (1/1).

    PubMed

    Cavallo, Gabriella; Marras, Giovanni; Metrangolo, Pierangelo; Pilati, Tullio; Terraneo, Giancarlo

    2013-03-01

    In the crystal structure of the title compound, C8F16I2·C14H8N2, the mol-ecules form infinite chains parallel to [2-11] through two symmetry-independent C-I⋯N halogen bonds (XBs). As commonly found, the perfluoro-alkyl mol-ecules segregate from the hydro-carbon ones, forming a layered structure. Apart from the XBs, the only contact below the sum of van der Waals radii is a weak H⋯F contact. The topology of the network is a nice example of the paradigm of the expansion of ditopic starting modules; the XB leads to the construction of infinite supramolecular chains along [2-11] formed by alternating XB donors and acceptors. PMID:23476525

  7. Invatamantul cu Predarea in Limbile Minoritatilor Nationale din Romania. In Anul Scolar 1992/1993 = The Education System in Romania: Tuition in the Languages of Ethnic Minorities. The School Year 1992/1993 = L'enseignement dispense dans les langues des minorites nationales de Roumanie. L'annee scolaire 1992/1993.

    ERIC Educational Resources Information Center

    Council for National Minorities, Bucharest (Romania).

    Documentation of the Romanian system of language instruction in ethnic minority languages is presented in Romanian, French, and English. It consists of: relevant provisions from the national constitution concerning the rights of ethnic minority members; organization and functioning of the educational system at the elementary, middle, and secondary…

  8. Análisis de la evolución química de los cúmulos abiertos de la Galaxia a través de simulaciones dinámicas con procesos de destrucción

    NASA Astrophysics Data System (ADS)

    Bignone, L. A.; Pellizza, L. J.; Piatti, A. E.; Tecce, T. E.

    It is well known that open clusters are excellent tracers (in time and space) of the metallicity of the Galactic disk. We analyze the history of stellar for- mation of the Galactic disk using numerical simulations of the dynamical evolution of the open cluster system. We have included the effects of clus- ter disruption caused by stellar evolution, tidal fields, and the interaction with the spiral arms. We model the present astrophysical properties of open clusters from initial hypothesis regarding their formation history. FULL TEXT IN SPANISH

  9. [Interpretation of spatial distribution pattern for dissolved inorganic nitrogen concentration in coastal estuary using hyperspectral data].

    PubMed

    Zhang, Dong; Xu, Yong; Zhang, Ying; Li, Huan

    2010-06-01

    Choosing dissolved inorganic nitrogen (DIN) as one of the representative nutritional salt monitoring indexes, a hyperspectral remotely sensed inversion model was built and applied to quantitatively retrieve water quality parameters with its spatial distribution patterns in coastal estuary with high suspended sediment concentration (SSC). It was found that when SSC was larger than 0.1 kg/m3, DIN concentration had a notable inverse correlation with SSC and the correlation coefficient R2 reached 0.617. Based on this conclusion, firstly the in-situ observed water surface remote sensing reflectance was resampled according to the spectral response characters of Hyperion sensor. And then, statistical correlation analysis between reflectance and DIN concentration was carried out. The results showed that band reflectance of R804 and R630 representing the second and first reflectance peak of water spectrum curve were sensitive to the variation of DIN concentration. And then, a pseudo remotely sensed sand parameter index R804 x R630/(R804 - R630) was calculated for the construction of the nonlinear DIN quantitative reversion model. Correlation coefficient R2 between observed and simulated DIN concentrations for 29 calibrating samples and 10 validating samples were 0.746 and 0.67, while their mean absolute errors reached 109.07 and 147.58 microg/L, respectively. The model was then applied on Hyperion hyperspectral image to get the spatial distribution character of DIN concentration in Sheyanghe river estuary and the DIN concentration was between 52 to 513 microg/L. Results indicated that in coastal estuary which was dominated by suspended sediments, the diffusive trends of DIN concentration reversed by remote sensing techniques had an intimate relationship with motions of tidal current and transportation attributes of SSC. As the hydrodynamic conditions were unclear, hyperspectral remote sensing technique was an effective technical way for dynamic survey of DIN concentration

  10. Nitrogen export from a boreal stream network following forest harvesting: seasonal nitrate removal and conservative export of organic forms

    NASA Astrophysics Data System (ADS)

    Schelker, J.; Sponseller, R.; Ring, E.; Högbom, L.; Löfgren, S.; Laudon, H.

    2016-01-01

    Clear-cutting is today the primary driver of large-scale forest disturbance in boreal regions of Fennoscandia. Among the major environmental concerns of this practice for surface waters is the increased mobilization of nutrients, such as dissolved inorganic nitrogen (DIN) into streams. But while DIN loading to first-order streams following forest harvest has been previously described, the downstream fate and impact of these inputs is not well understood. We evaluated the downstream fate of DIN and dissolved organic nitrogen (DON) inputs in a boreal landscape that has been altered by forest harvests over a 10-year period. The small first-order streams indicated substantial leaching of DIN, primarily as nitrate (NO3-) in response to harvests with NO3- concentrations increasing by ˜ 15-fold. NO3- concentrations at two sampling stations further downstream in the network were strongly seasonal and increased significantly in response to harvesting at the mid-sized stream, but not at the larger stream. DIN removal efficiency, Er, calculated as the percentage of "forestry derived" DIN that was retained within the stream network based on a mass-balance model was highest during the snowmelt season followed by the growing season, but declined continuously throughout the dormant season. In contrast, export of DON from the landscape indicated little removal and was essentially conservative. Overall, net removal of DIN between 2008 and 2011 accounted for ˜ 65 % of the total DIN mass exported from harvested patches distributed across the landscape. These results highlight the capacity of nitrogen-limited boreal stream networks to buffer DIN mobilization that arises from multiple clear-cuts within this landscape. Further, these findings shed light on the potential impact of anticipated measures to increase forest yields of boreal forests, such as increased fertilization and shorter forest rotations, which may increase the pressure on boreal surface waters in the future.

  11. Locomotor rhythm maintenance: electrical coupling among premotor excitatory interneurons in the brainstem and spinal cord of young Xenopus tadpoles

    PubMed Central

    Li, Wen-Chang; Roberts, Alan; Soffe, Stephen R

    2009-01-01

    Electrical coupling is important in rhythm generating systems. We examine its role in circuits controlling locomotion in a simple vertebrate model, the young Xenopus tadpole, where the hindbrain and spinal cord excitatory descending interneurons (dINs) that drive and maintain swimming have been characterised. Using simultaneous paired recordings, we show that most dINs are electrically coupled exclusively to other dINs (DC coupling coefficients ∼8.5%). The coupling shows typical low-pass filtering. We found no evidence that other swimming central pattern generator (CPG) interneurons are coupled to dINs or to each other. Electrical coupling potentials between dINs appear to contribute to their unusually reliable firing during swimming. To investigate the role of electrical coupling in swimming, we evaluated the specificity of gap junction blockers (18-β-GA, carbenoxolone, flufenamic acid and heptanol) in paired recordings. 18-β-GA at 40–60 μm produced substantial (84%) coupling block but few effects on cellular properties. Swimming episodes in 18-β-GA were significantly shortened (to ∼2% of control durations). At the same time, dIN firing reliability fell from nearly 100% to 62% of swimming cycles and spike synchronization weakened. Because dINs drive CPG neuron firing and are critical in maintaining swimming, the weakening of dIN activity could account for the effects of 18-β-GA on swimming. We conclude that electrical coupling among pre motor reticulospinal and spinal dINs, the excitatory interneurons that drive the swimming CPG in the hatchling Xenopus tadpole, may contribute to the maintenance of swimming as well as synchronization of activity. PMID:19221124

  12. Characterising the fate of nitrogenous waste from the sea-cage aquaculture of spiny lobsters using numerical modelling.

    PubMed

    Lee, Soxi; Hartstein, Neil D; Jeffs, Andrew

    2015-06-01

    Although the aquaculture of spiny lobsters has been expanding since the 1970s, very little is known about the potential environmental impacts on water quality of this activity. This study quantified the production of dissolved inorganic nitrogen (DIN) from Australasian red spiny lobsters, Jasus edwardsii, in the laboratory, and these data were then used in a numerical model to predict the dispersal pattern of DIN from a hypothetical commercial spiny lobster farm for a coastal site where such a farm would typically be located. Modelling scenarios were set up with combinations of two different stocking densities (3 and 5 kg m(-3)), two different diets (mussels and moist artificial diet) and three different feed conversion ratios (FCR = 3, 5 and 28). DIN excretion rate from unfed lobsters in the laboratory on average was 1.10 ± 0.12 μg N g(-1) h(-1) while feeding lobsters on mussels and artificial diet increased DIN excretion significantly by around eightfold and twofold, respectively. Ammonia was consistently the dominant contributor to measured DIN output from lobsters. Modelling results indicated that the mean elevated DIN from a hypothetical farm where the lobsters were fed with mussels ranged from 7 up to 20 μg N L(-1) with increasing stocking density and FCR and was 30-150 % higher than the mean elevated DIN resulting from lobsters fed with artificial diet. Overall, the results indicated that DIN output from the hypothetical spiny lobster sea-cage farming is unlikely to be problematic using the FCR, stocking density, and the number of cages modelled at the coastal site in this study. Furthermore, feeding lobsters with artificial diet can help maintain a lower DIN output than seafood, such as mussels or trash fish. PMID:25601612

  13. Early results from an effort to downscale a global dissolved inorganic nitrogen model to achieve a regional assessment of nitrogen dynamics in the Columbia River Basin

    NASA Astrophysics Data System (ADS)

    Miller, C. C.; Harrison, J.

    2013-12-01

    Excessive nitrogen (N) export to coastal systems has increased dramatically since the early 20th century. The increase in N has been linked to significant environmental impacts such as eutrophication, fish kills, and harmful algal blooms and is caused in part by the increasing use and quantity of synthetic fertilizer on farmland. Significant portions of both the Willamette River Valley in Oregon and the Palouse region of eastern Washington are agricultural land, approximately 20% and 57% respectively. Nitrogen in the form of dissolved inorganic nitrogen (DIN) can leach from farms and pasture land into ground and surface water systems. This leaching, combined with DIN in runoff, contributes to the environmental degradation of both waterways (i.e. streams, rivers) and coastal estuaries. Because of this it is important to understand what effects changes in DIN application will have on water quality and DIN export to the coast. DIN export data, retrieved from the U.S. Geological Survey National Water Information System, was analyzed for 23 major subbasins in the Columbia River Basin (CRB) and estimates DIN export (per area yield) ranging from 5.0 to 883.1 kg N km-2 yr-1. Here we present early results from our effort to downscale the Global Nutrient Export from WaterSheds (Global NEWS) DIN model for application within the Columbia River Basin (CRB). This first attempt at downscaling Global NEWS is missing some key higher-resolution N inputs for the model as well as accurate dam retention and runoff factors which could account for the low correlation between model output and observed data (R2 = 0.21).Our regional model predicts DIN yields ranging from 7.9 to 1146.6 kg N km-2 yr-1. Both the model output and observed data predict the highest per area DIN yields occurring in the Willamette river subbasin. Total DIN export to the coast was modeled as 0.06 Tg N yr-1 compared to 0.07 Tg N yr-1 calculated from the measured data. Based on current model inputs biological N2

  14. Assessing dissolved inorganic nitrogen flux in the Yangtze River, China: Sources and scenarios

    NASA Astrophysics Data System (ADS)

    Xu, Hao; Chen, Zhongyuan; Finlayson, Brian; Webber, Michael; Wu, Xiaodan; Li, Maotian; Chen, Jing; Wei, Taoyuan; Barnett, Jon; Wang, Mark

    2013-07-01

    This study gives a thorough assessment of the occurrences of dissolved inorganic nitrogen (DIN) in the Yangtze River in the past half century. The results have shown that nitrogen fertilizer, a major DIN source, has been replaced by domestic sewage in the last decade, which has dramatically driven up DIN loads in the Yangtze. DIN concentrations showed a rapid increase from < 0.5 mg L- 1 in the 1960s to nearly 1.5 mg L- 1 at the end of the 1990s. Since then DIN has remained steady at ca. 1.6-1.8 mg L- 1. A significant relationship between the historical DIN record at the downstream gauging station (Datong) and nitrogen (N) sources in the Yangtze River basin is established using principal components analysis. This allows us to apportion DIN loads for the year 2007 (the most recent year of measured DIN data available) to various N sources, listed here in order of weight: sewage (0.391 million tons); atmosphere (0.358 million tons); manure (0.318 million tons), N-fertilizer (0.271 million tons). Therefore, we estimated that a DIN load of 1.339 × 106 t was delivered to the lower Yangtze and its estuarine water in that year. We established scenarios to predict DIN concentrations in the lower Yangtze at 10 year intervals to 2050. For a dry year (20,000 m3 s- 1) DIN concentrations would range from 2.2-3.0 mg L- 1 for 2020-2050. This far exceeds the 2.0 mg L- 1 defined on the Chinese National Scale as the worst class for potable source water. The scenario results suggest that upgrading the sewage treatment systems throughout the basin will be an effective way to help reduce DIN concentrations to less than 2.0 mg L- 1 in the lower Yangtze. This would save the Shanghai megacity from the increasing threat of heavily polluted water sources, where > 23 million people are at present dependent on the Yangtze estuary for 70% of their freshwater intake.

  15. EFFECT OF FLUID SHEAR AND IRRADIANCE ON POPULATION GROWTH AND CELLULAR TOXIN CONTENT OF THE DINOFLAGELLATE ALEXANDRIUM FUNDYENSE.

    EPA Science Inventory

    The potential for in situ turbulence to inhibit dinoflagellate population growth has been demonstrated by experimentally exposing dinoflagellate cultures to quantified shear flow. However, despite interest in understanding environmental factors that affect the growth of toxic din...

  16. DISTINGUISHING AND QUANTIFYING NATURAL AND ANTHROPOGENIC NUTRIENT LOADING TO PACIFIC NORTHWEST ESTUARIES

    EPA Science Inventory

    Many coastal ecosystems are experiencing environmental problems due to excess nutrients, particularly nitrogen. Identifying the sources of dissolved inorganic nitrogen (DIN) in estuaries is complicated by the multiple sources and pathways, temporal variability in inputs, and var...

  17. Chemical control of photochromism and a multiresponsive molecular switch based on a diarylethene derivative containing naphthol.

    PubMed

    Liu, Wenju; Li, Ziyong; Hu, Fang; Yin, Jun; Yu, Guang-Ao; Liu, Sheng Hua

    2014-12-01

    A photochemically inactive diarylethene derivative with a naphthol group bridging through the imidazole unit (DIN) was synthesized to characterize a multifunctional switch. Upon stimulation by radiation (UV and visible light), the target diarylethene derivative DIN was not photochemically inactive but photo-active, it could be photo-converted into a keto compound. Moreover, the photochromic reaction of DIN was observed when it was stimulated by the addition of acid or methylation. Fluorescence quenching and the bathochromic effect occurred when DIN was stimulated by the addition of copper ions and a base, respectively. Its optical properties could be regulated by various chemical stimuli. Thus, it can potentially be utilized as a multiresponsive molecular switch. PMID:25341073

  18. Factors influencing export of dissolved inorganic nitrogen by major rivers: A new seasonal, global-scale model

    EPA Science Inventory

    Understanding sub-annual patterns of catchment dissolved inorganic nitrogen (DIN) export is critical for predicting and mitigating impacts of coastal eutrophication, such as algal blooms and hypoxic areas, which are often seasonal phenomena. We developed the first calibrated glob...

  19. Effect of enhanced reactive nitrogen availability on plant-sediment mediated degradation of polycyclic aromatic hydrocarbons in contaminated mangrove sediment.

    PubMed

    Jiang, Shan; Lu, Haoliang; Zhang, Qiong; Liu, JingChun; Yan, Chongling

    2016-02-15

    As land-ocean interaction zones, mangrove systems receive substantial polycyclic aromatic hydrocarbons (PAHs) from sewage and combustion of fossil fuel. In this study, we investigated the relationship between dissolved inorganic nitrogen (DIN) availability and degradation rate of phenanthrene, a typical PAH compound, in mangrove plant-sediment systems, using Avicennia marina as a model plant. After 50day incubation, phenanthrene removal ratios in sediments ranged from 53.8% to 97.2%. In non-rhizosphere sediment, increasing DIN accessibility increased microbial biomass and total microbial activity, while enhancements in population size of phenanthrene degradation bacteria (PDB) and phenanthrene degradation rates were insignificant. In contrast, the presence of excessive DIN in rhizosphere sediment resulted in a significantly large number of PDB, leading to a rapid dissipation rate of phenanthrene. The differences in degradation rates and abundances of degrader in sediment may be explained by the enhanced root activity due to the elevation in DIN accessibility. PMID:26749225

  20. Blessed by the Holy People.

    PubMed

    House, Carrie

    2016-01-01

    Men-women and women-men have a long tradition in Diné (Navajo) culture where they were, and sometimes still are, held in high esteem. Their supernatural prototypes figure prominently in parts of the Diné Origin Story. It is in this cosmological worldview and tradition of acceptance that Carrie, a multi-dualistic spirit, grew up to be a female-bodied man supported and respected by his/her family and community. He/she has worked in various professions defined as "masculine" in Diné and Western contexts, such as fire fighter, heavy equipment operator, truck driver, and building contractor. In addition, Carrie is a keeper of Diné traditions, and has also been active educating Native and Non-Native audiences on 2SpiritLesbianGayBiTransIntersexQueer issues. PMID:27254759

  1. Eutrophication and macroalgal blooms in temperate and tropical coastal waters: nutrient enrichment experiments with Ulva spp.

    PubMed Central

    Teichberg, Mirta; Fox, Sophia E; Olsen, Ylva S; Valiela, Ivan; Martinetto, Paulina; Iribarne, Oscar; Muto, Elizabeti Yuriko; Petti, Monica A V; Corbisier, Thaïs N; Soto-Jiménez, Martín; Páez-Osuna, Federico; Castro, Paula; Freitas, Helena; Zitelli, Andreina; Cardinaletti, Massimo; Tagliapietra, Davide

    2010-01-01

    Receiving coastal waters and estuaries are among the most nutrient-enriched environments on earth, and one of the symptoms of the resulting eutrophication is the proliferation of opportunistic, fast-growing marine seaweeds. Here, we used a widespread macroalga often involved in blooms, Ulva spp., to investigate how supply of nitrogen (N) and phosphorus (P), the two main potential growth-limiting nutrients, influence macroalgal growth in temperate and tropical coastal waters ranging from low- to high-nutrient supplies. We carried out N and P enrichment field experiments on Ulva spp. in seven coastal systems, with one of these systems represented by three different subestuaries, for a total of nine sites. We showed that rate of growth of Ulva spp. was directly correlated to annual dissolved inorganic nitrogen (DIN) concentrations, where growth increased with increasing DIN concentration. Internal N pools of macroalgal fronds were also linked to increased DIN supply, and algal growth rates were tightly coupled to these internal N pools. The increases in DIN appeared to be related to greater inputs of wastewater to these coastal waters as indicated by high δ15N signatures of the algae as DIN increased. N and P enrichment experiments showed that rate of macroalgal growth was controlled by supply of DIN where ambient DIN concentrations were low, and by P where DIN concentrations were higher, regardless of latitude or geographic setting. These results suggest that understanding the basis for macroalgal blooms, and management of these harmful phenomena, will require information as to nutrient sources, and actions to reduce supply of N and P in coastal waters concerned.

  2. Detecting, mapping and monitoring of land subsidence in Jharia Coalfield, Jharkhand, India by spaceborne differential interferometric SAR, GPS and precision levelling techniques

    NASA Astrophysics Data System (ADS)

    Chatterjee, R. S.; Thapa, Shailaja; Singh, K. B.; Varunakumar, G.; Raju, E. V. R.

    2015-08-01

    The study aims at detection, mapping and monitoring of land subsidence in Jharia Coalfield, Jharkhand, India by spaceborne DInSAR, GPS and precision levelling techniques. Using multi-frequency C- and L-band DInSAR, both slowly and rapidly subsiding areas were identified and DInSAR-based subsidence maps were prepared. C-band DInSAR was found useful for detection of slowly subsiding areas whereas L-band DInSAR for rapidly subsiding and/or adverse land cover areas. Due to dynamic nature of mining and adverse land cover, temporal decorrelation poses a serious problem particularly in C-band DInSAR. Specially designed InSAR coherence guided adaptive filtering was found useful to highlight the deformation fringes. Collateral GPS and levelling observations were conducted in three test sites to validate DInSAR measurements and to determine the net displacement vectors. We observed an appreciable horizontal displacement component of land subsidence in all the test sites. For comparison of results, we calculated InSAR coherence weighted LOS displacement rates from the unwrapped differential interferograms of smaller spatial subsets and LOS projected ground-based displacement rates in three test sites. We found good agreement between DInSAR and ground-based measurements except for C-band observation in Dobari test site primarily due to large difference in observation periods and temporally inconsistent land subsidence. Collateral spaceborne and ground-based observations were also found useful for characterization of subsidence phenomena to determine net displacement vector and horizontal displacement component. In coal mining areas with spatially scattered and temporally irregular land subsidence phenomena, the adopted methodology can be used successfully for detection, mapping and monitoring of the subsiding areas vulnerable to future collapse. This will facilitate efficient planning and designing of surface infrastructures and other developmental structures in the mining

  3. Process Models in E-Learning--Bottom-Up or Top-Down?

    ERIC Educational Resources Information Center

    Sahl, Sarah; Martens, Alke

    2015-01-01

    In the paper, different approaches of process modelling in e-learning system development are investigated. We provide a look at the DIN PAS 1032-1 and in the process model ROME, which is a derivation of the DIN PAS 1032-1. ROME has been extended by several pattern approaches. However, after several years of using ROME, we found out that ROME has…

  4. Coal mining induced land subsidence monitoring using multiband spaceborne differential interferometric synthetic aperture radar data

    NASA Astrophysics Data System (ADS)

    Yue, Huanyin; Liu, Guang; Guo, Huadong; Li, Xinwu; Kang, Zhizhong; Wang, Runfeng; Zhong, Xuelian

    2011-01-01

    The differential interferometric synthetic aperture radar (SAR)(DInSAR) technique has been applied to the earth surface deformation monitoring in many areas. In this paper, the DInSAR technique is used to process the spaceborne SAR data including C band ENVISAT ASAR, L band JERS SAR, and ALOS PALSAR data to derive the temporal land subsidence information in the Fengfeng coal mine area, Hebei province in China. Since JERS and ALOS do not have precise orbit, an orbit adjustment must be accomplished before the DInSAR interferogram was formed. Twenty-three differential interferograms are derived to show the temporal change of the land subsidence range and position. At the acquisition time of ENVISAT ASAR, the leveling in the Dashucun coal mine in Fengfeng area was carried, the historical excavation data in 8 coal mines in Fengfeng area from 1992 to 2007 were collected as well. In our analysis, the DInSAR results are compared with leveling data and historical excavation data. The comparison results show the DInSAR subsidence results are consistent with the leveling results and the historical excavation data, and the L band DInSAR shows more advantages than C band in the coal mining induced subsidence monitoring in a rural area. The feasibility and limitations in coal mining induced subsidence monitoring with DInSAR are analyzed, and the possibility of underground mining activity monitoring by spaceborne InSAR data is evaluated. The experimental results show that both C and L band can accomplish monitoring mining area subsidence, but C band has more restricted conditions of its perpendicular baseline. In order to get a satisfactory outcome in mining area subsidence by the DInSAR method, the time series of SAR images of every visit and SAR deformation interferograms should be archived.

  5. Inorganic nitrogen wet deposition: Evidence from the North-South Transect of Eastern China.

    PubMed

    Zhan, X; Yu, G; He, N; Jia, B; Zhou, M; Wang, C; Zhang, J; Zhao, G; Wang, S; Liu, Y; Yan, J

    2015-09-01

    We examined the spatio-temporal variation of dissolved inorganic nitrogen (DIN) deposition in eight typical forest ecosystems of Eastern China for three consecutive years. DIN deposition exhibited an increasing gradient from north to south, with N-NH4(+) as the predominant contributor. DIN deposition in precipitation changed after interaction with the forest canopy, and serious ecological perturbations are expected in this region. DIN deposition presented seasonal fluctuations, which might be ascribed to agricultural activity, fossil-fuel combustion and environmental factors (i.e., wind direction, soil temperature). Notably, N fertilizer use (FN), energy consumption (E), and precipitation (P) jointly explained 84.3% of the spatial variation in DIN deposition, of which FN (27.2%) was the most important, followed by E (24.8%), and finally P (9.3%). The findings demonstrate that DIN deposition is regulated by precipitation mainly via anthropogenic N emissions, and this analysis provides decision-makers a novel view for N pollution abatement. PMID:25898231

  6. Selection of effective macroalgal species and tracing nitrogen sources on the different part of Yantai coast, China indicated by macroalgal δ(15)N values.

    PubMed

    Wang, Yujue; Liu, Dongyan; Richard, Pierre; Di, Baoping

    2016-01-15

    To determine the dominant nitrogen sources and select effective macroalgal species for monitoring eutrophication along the Yantai coast, the total carbon (TOC), total nitrogen (TN) and nitrogen stable isotope ratio (δ(15)N) in macroalgal tissue were analyzed in conjunction with environmental variables in seawater along the Yantai coastline. The ranges of macroalgal tissue δ(15)N values together with dissolved inorganic nitrogen (DIN) composition indicated that except for the atmospheric deposition, there were three dominant types of nitrogen sources along the Yantai coast, with the agricultural fertilizer usage and factorial wastewater input at the S1 (Zhifu Island coast), the sewage discharge at S2 (the Moon Bay coast), the sewage discharge together with aquaculture impacts at S3 (Fisherman Wharf coast) and S4 (the Horse Island coast). Macroalgal growth were not limited by DIN but limited by P at S2, S3 and S4. Macroalgal species suitable or not for DIN source tracing along the Yantai coast were discussed. For sites with low DIN concentration, many species of three phyla could be used for DIN sources tracing with Laurencia okamurai, Gloiopeltis furcata and Ulva pertusa being ideal species. For site with high DIN concentration, however, species of Rhodophyta were not suitable and only Scytosiphon lomentaria and Monostroma nitidium were chosen. PMID:26519590

  7. Independent signaling by Drosophila insulin receptor for axon guidance and growth

    PubMed Central

    Li, Caroline R.; Guo, Dongyu; Pick, Leslie

    2014-01-01

    The Drosophila insulin receptor (DInR) regulates a diverse array of biological processes including growth, axon guidance, and sugar homeostasis. Growth regulation by DInR is mediated by Chico, the Drosophila homolog of vertebrate insulin receptor substrate proteins IRS1–4. In contrast, DInR regulation of photoreceptor axon guidance in the developing visual system is mediated by the SH2-SH3 domain adaptor protein Dreadlocks (Dock). In vitro studies by others identified five NPXY motifs, one in the juxtamembrane region and four in the signaling C-terminal tail (C-tail), important for interaction with Chico. Here we used yeast two-hybrid assays to identify regions in the DInR C-tail that interact with Dock. These Dock binding sites were in separate portions of the C-tail from the previously identified Chico binding sites. To test whether these sites are required for growth or axon guidance in whole animals, a panel of DInR proteins, in which the putative Chico and Dock interaction sites had been mutated individually or in combination, were tested for their ability to rescue viability, growth and axon guidance defects of dinr mutant flies. Sites required for viability were identified. Unexpectedly, mutation of both putative Dock binding sites, either individually or in combination, did not lead to defects in photoreceptor axon guidance. Thus, either sites also required for viability are necessary for DInR function in axon guidance and/or there is redundancy built into the DInR/Dock interaction such that Dock is able to interact with multiple regions of DInR. We also found that simultaneous mutation of all five NPXY motifs implicated in Chico interaction drastically decreased growth in both male and female adult flies. These animals resembled chico mutants, supporting the notion that DInR interacts directly with Chico in vivo to control body size. Mutation of these five NPXY motifs did not affect photoreceptor axon guidance, segregating the roles of DInR in the

  8. Independent signaling by Drosophila insulin receptor for axon guidance and growth.

    PubMed

    Li, Caroline R; Guo, Dongyu; Pick, Leslie

    2013-01-01

    The Drosophila insulin receptor (DInR) regulates a diverse array of biological processes including growth, axon guidance, and sugar homeostasis. Growth regulation by DInR is mediated by Chico, the Drosophila homolog of vertebrate insulin receptor substrate proteins IRS1-4. In contrast, DInR regulation of photoreceptor axon guidance in the developing visual system is mediated by the SH2-SH3 domain adaptor protein Dreadlocks (Dock). In vitro studies by others identified five NPXY motifs, one in the juxtamembrane region and four in the signaling C-terminal tail (C-tail), important for interaction with Chico. Here we used yeast two-hybrid assays to identify regions in the DInR C-tail that interact with Dock. These Dock binding sites were in separate portions of the C-tail from the previously identified Chico binding sites. To test whether these sites are required for growth or axon guidance in whole animals, a panel of DInR proteins, in which the putative Chico and Dock interaction sites had been mutated individually or in combination, were tested for their ability to rescue viability, growth and axon guidance defects of dinr mutant flies. Sites required for viability were identified. Unexpectedly, mutation of both putative Dock binding sites, either individually or in combination, did not lead to defects in photoreceptor axon guidance. Thus, either sites also required for viability are necessary for DInR function in axon guidance and/or there is redundancy built into the DInR/Dock interaction such that Dock is able to interact with multiple regions of DInR. We also found that simultaneous mutation of all five NPXY motifs implicated in Chico interaction drastically decreased growth in both male and female adult flies. These animals resembled chico mutants, supporting the notion that DInR interacts directly with Chico in vivo to control body size. Mutation of these five NPXY motifs did not affect photoreceptor axon guidance, segregating the roles of DInR in the

  9. A Computational Model of a Descending Mechanosensory Pathway Involved in Active Tactile Sensing.

    PubMed

    Ache, Jan M; Dürr, Volker

    2015-07-01

    Many animals, including humans, rely on active tactile sensing to explore the environment and negotiate obstacles, especially in the dark. Here, we model a descending neural pathway that mediates short-latency proprioceptive information from a tactile sensor on the head to thoracic neural networks. We studied the nocturnal stick insect Carausius morosus, a model organism for the study of adaptive locomotion, including tactually mediated reaching movements. Like mammals, insects need to move their tactile sensors for probing the environment. Cues about sensor position and motion are therefore crucial for the spatial localization of tactile contacts and the coordination of fast, adaptive motor responses. Our model explains how proprioceptive information about motion and position of the antennae, the main tactile sensors in insects, can be encoded by a single type of mechanosensory afferents. Moreover, it explains how this information is integrated and mediated to thoracic neural networks by a diverse population of descending interneurons (DINs). First, we quantified responses of a DIN population to changes in antennal position, motion and direction of movement. Using principal component (PC) analysis, we find that only two PCs account for a large fraction of the variance in the DIN response properties. We call the two-dimensional space spanned by these PCs 'coding-space' because it captures essential features of the entire DIN population. Second, we model the mechanoreceptive input elements of this descending pathway, a population of proprioceptive mechanosensory hairs monitoring deflection of the antennal joints. Finally, we propose a computational framework that can model the response properties of all important DIN types, using the hair field model as its only input. This DIN model is validated by comparison of tuning characteristics, and by mapping the modelled neurons into the two-dimensional coding-space of the real DIN population. This reveals the versatility

  10. Geochemistry of inorganic nitrogen in waters released from coal-bed natural gas production wells in the powder river basin, wyoming

    USGS Publications Warehouse

    Smith, R.L.; Repert, D.A.; Hart, C.P.

    2009-01-01

    Water originating from coal-bed natural gas (CBNG) production wells typically contains ammonium and is often disposed via discharge to ephemeral channels. A. study conducted in the Powder River Basin, Wyoming, documented downstream changes in CBNG water composition, emphasizing nitrogen-cycling processes and the fate of ammonium. Dissolved ammonium concentrations from 19 CBNG discharge points ranged from 95 to 527 ??M. Within specific channels, ammonium concentrations decreased with transport distance, with subsequent increases in nitrite and nitrate concentrations. Removal efficiency, or uptake, of total dissolved inorganic nitrogen (DIN) varied between channel types. DIN uptake was greater in the gentle-sloped, vegetated channel as compared to the incised, steep, and sparsely vegetated channel and was highly correlated with diel patterns of incident light and dissolved oxygen concentration. In a larger main channel with multiple discharge inputs (n = 13), DIN concentrations were >300 ??M, with pH > 8.5, after 5 km of transport. Ammonium represented 25-30% of the large-channel DIN, and ammonium concentrations remained relatively constant with time, with only a weak diel pattern evident. In July 2003, the average daily large-channel DIN load was 23 kg N day-1 entering the Powder River, an amount which substantially increased the total Powder River DIN load after the channel confluence. These results suggest that CBNG discharge may be an important source of DIN to western watersheds, at least at certain times of the year, and that net oxidation and/or removal is dependent upon the extent of contact with sediment and biomass, type of drainage channel, and time of day. ?? 2009 American Chemical Society.

  11. Geochemistry of Inorganic Nitrogen in Waters Released from Coal-Bed Natural Gas Production Wells in the Powder River Basin, Wyoming

    USGS Publications Warehouse

    Smith, Richard L.; Repert, Deborah A.; Hart, Charles P.

    2009-01-01

    Water originating from coal-bed natural gas (CBNG) production wells typically contains ammonium and is often disposed via discharge to ephemeral channels. A study conducted in the Powder River Basin, Wyoming, documented downstream changes in CBNG water composition, emphasizing nitrogen-cycling processes and the fate of ammonium. Dissolved ammonium concentrations from 19 CBNG discharge points ranged from 95 to 527 µM. Within specific channels, ammonium concentrations decreased with transport distance, with subsequent increases in nitrite and nitrate concentrations. Removal efficiency, or uptake, of total dissolved inorganic nitrogen (DIN) varied between channel types. DIN uptake was greater in the gentle-sloped, vegetated channel as compared to the incised, steep, and sparsely vegetated channel and was highly correlated with diel patterns of incident light and dissolved oxygen concentration. In a larger main channel with multiple discharge inputs (n = 13), DIN concentrations were >300 µM, with pH > 8.5, after 5 km of transport. Ammonium represented 25-30% of the large-channel DIN, and ammonium concentrations remained relatively constant with time, with only a weak diel pattern evident. In July 2003, the average daily large-channel DIN load was 23 kg N day-1 entering the Powder River, an amount which substantially increased the total Powder River DIN load after the channel confluence. These results suggest that CBNG discharge may be an important source of DIN to western watersheds, at least at certain times of the year, and that net oxidation and/or removal is dependent upon the extent of contact with sediment and biomass, type of drainage channel, and time of day.

  12. The effect of Landscape on Riverine Dissolved Inorganic Nitrogen Yield in populous watershed in the Danshui River in Taiwan

    NASA Astrophysics Data System (ADS)

    Shih, Yu-Ting; Lee, Tsung-Yu; Huang, -Chuan, Jr.

    2015-04-01

    This study combines the observed riverine DIN (dissolved inorganic nitrogen) export and the controlling factors (land-use, population and discharge) to inversely estimate the effective DIN yield factors for individual land-use and DIN per capita loading. A total of 16 sub-catchments, with different land-use compositions on the Danshui River of Taiwan, were used in this study. Observed riverine DIN concentrations and yields varied from 20 - 450 μM and 400 - 10,000 kg-N km-2 yr-1 corresponding to the increase of urbanization gradient (e.g. building and population). Meanwhile, the transport behaviors changed from hydrological enhancement to dilution with increasing urbanization as well. Our method shows that the DIN yield factors, independent of discharge, are 12.7, 63.9, and 1381.0 μM, for forest, agriculture, and building, respectively, which equals to 444.5, 2236.5, 48,335 kg-N km-2 yr-1 at the given annual runoff of 2,500 mm. The agriculture DIN yield only accounts for 10% of fertilizer application indicating the complicated N cascade and possible over fertilization. The DIN per capita loading (~0.49 kg-N Capita-1 yr-1) which is lower than the documented human N emission (1.6 - 5.5 kg-N Capita-1 yr-1) can be regarded as an effective export coefficient after treatment or retention. A conducted scenario experiment supports the observations demonstrating the capability for assessment. We therefore, can extrapolate all possible combinations of land-use, discharge, and population density for evaluation. This can provide a strong basis for watershed management and supplementary estimation for regional to global study.

  13. Variation characteristics of nitrogen concentrations through forest hydrologic subcycles in various forests across mainland China.

    PubMed

    Sun, Suqi; Wang, Yunqi; Wang, Yujie; Zhang, Huilan; Yu, Lei; Liu, Yong; Zhu, Jinqi

    2015-01-01

    Increased anthropogenic nitrogen emissions and more severe environmental issues (e.g. air pollution, soil acidification, and plant nutrient imbalances) are striking forest ecosystems. Data on NH4+ and NO3- concentrations in throughfall and stemflow were collected to estimate variation characteristics of nitrogen concentrations through forest hydrological processes across China. A typical study was carried out in the three forest types in the Jinyun Mountain region of Chongqing, from May to October 2012. Nitrogen concentrations in throughfall and stemflow are higher than those in atmospheric precipitation. DIN concentrations in atmospheric precipitation, throughfall, and stemflow, across China and in the Jinyun Mountain region, were 2.18 and 1.51, 3.19 and 3.88, and 5.14 and 3.92 mg N L(-1), respectively. NH4+ concentration was higher than NO3- concentration, suggesting NH4+ is the dominant nitrogen component in China. Additionally, across China, a linear relationship existed between DIN and NH4+, and between DIN and NO3- in atmospheric precipitation. DIN concentrations in throughfall and stemflow changed with the observed changes in precipitation, and DIN concentrations in precipitation positively correlated with those in throughfall and in stemflow were also observed. Moreover, average DIN concentrations in throughfall and stemflow varied in different forest types, resulting from differences in forest canopy structures and tree species characteristics. In the Jinyun Mountain region, both throughfall and stemflow DIN concentrations were the highest in the mixed broadleaved/coniferous forest, followed by evergreen broadleaved forest, and the lowest in moso bamboo forest. Monthly variations of NH4+ and NO3- concentrations, in throughfall and stemflow, were observed in the Jinyun Mountain region. PMID:25686284

  14. Toxin YafQ Reduces Escherichia coli Growth at Low Temperatures.

    PubMed

    Zhao, Yueju; McAnulty, Michael J; Wood, Thomas K

    2016-01-01

    Toxin/antitoxin (TA) systems reduce metabolism under stress; for example, toxin YafQ of the YafQ/DinJ Escherichia coli TA system reduces growth by cleaving transcripts with in-frame 5'-AAA-G/A-3' sites, and antitoxin DinJ is a global regulator that represses its locus as well as controls levels of the stationary sigma factor RpoS. Here we investigated the influence on cell growth at various temperatures and found that deletion of the antitoxin gene, dinJ, resulted in both reduced metabolism and slower growth at 18°C but not at 37°C. The reduction in growth could be complemented by producing DinJ from a plasmid. Using a transposon screen to reverse the effect of the absence of DinJ, two mutations were found that inactivated the toxin YafQ; hence, the toxin caused the slower growth only at low temperatures rather than DinJ acting as a global regulator. Corroborating this result, a clean deletion of yafQ in the ΔdinJ ΔKmR strain restored both metabolism and growth at 18°C. In addition, production of YafQ was more toxic at 18°C compared to 37°C. Furthermore, by overproducing all the E. coli proteins, the global transcription repressor Mlc was found that counteracts YafQ toxicity only at 18°C. Therefore, YafQ is more effective at reducing metabolism at low temperatures, and Mlc is its putative target. PMID:27557125

  15. Toxin YafQ Reduces Escherichia coli Growth at Low Temperatures

    PubMed Central

    Zhao, Yueju; McAnulty, Michael J.

    2016-01-01

    Toxin/antitoxin (TA) systems reduce metabolism under stress; for example, toxin YafQ of the YafQ/DinJ Escherichia coli TA system reduces growth by cleaving transcripts with in-frame 5’-AAA-G/A-3’ sites, and antitoxin DinJ is a global regulator that represses its locus as well as controls levels of the stationary sigma factor RpoS. Here we investigated the influence on cell growth at various temperatures and found that deletion of the antitoxin gene, dinJ, resulted in both reduced metabolism and slower growth at 18°C but not at 37°C. The reduction in growth could be complemented by producing DinJ from a plasmid. Using a transposon screen to reverse the effect of the absence of DinJ, two mutations were found that inactivated the toxin YafQ; hence, the toxin caused the slower growth only at low temperatures rather than DinJ acting as a global regulator. Corroborating this result, a clean deletion of yafQ in the ΔdinJ ΔKmR strain restored both metabolism and growth at 18°C. In addition, production of YafQ was more toxic at 18°C compared to 37°C. Furthermore, by overproducing all the E. coli proteins, the global transcription repressor Mlc was found that counteracts YafQ toxicity only at 18°C. Therefore, YafQ is more effective at reducing metabolism at low temperatures, and Mlc is its putative target. PMID:27557125

  16. A Computational Model of a Descending Mechanosensory Pathway Involved in Active Tactile Sensing

    PubMed Central

    Ache, Jan M.; Dürr, Volker

    2015-01-01

    Many animals, including humans, rely on active tactile sensing to explore the environment and negotiate obstacles, especially in the dark. Here, we model a descending neural pathway that mediates short-latency proprioceptive information from a tactile sensor on the head to thoracic neural networks. We studied the nocturnal stick insect Carausius morosus, a model organism for the study of adaptive locomotion, including tactually mediated reaching movements. Like mammals, insects need to move their tactile sensors for probing the environment. Cues about sensor position and motion are therefore crucial for the spatial localization of tactile contacts and the coordination of fast, adaptive motor responses. Our model explains how proprioceptive information about motion and position of the antennae, the main tactile sensors in insects, can be encoded by a single type of mechanosensory afferents. Moreover, it explains how this information is integrated and mediated to thoracic neural networks by a diverse population of descending interneurons (DINs). First, we quantified responses of a DIN population to changes in antennal position, motion and direction of movement. Using principal component (PC) analysis, we find that only two PCs account for a large fraction of the variance in the DIN response properties. We call the two-dimensional space spanned by these PCs ‘coding-space’ because it captures essential features of the entire DIN population. Second, we model the mechanoreceptive input elements of this descending pathway, a population of proprioceptive mechanosensory hairs monitoring deflection of the antennal joints. Finally, we propose a computational framework that can model the response properties of all important DIN types, using the hair field model as its only input. This DIN model is validated by comparison of tuning characteristics, and by mapping the modelled neurons into the two-dimensional coding-space of the real DIN population. This reveals the

  17. Net anthropogenic nitrogen inputs (NANI) into the Yangtze River basin and the relationship with riverine nitrogen export

    NASA Astrophysics Data System (ADS)

    Chen, Fei; Hou, Lijun; Liu, Min; Zheng, Yanling; Yin, Guoyu; Lin, Xianbiao; Li, Xiaofei; Zong, Haibo; Deng, Fengyu; Gao, Juan; Jiang, Xiaofen

    2016-02-01

    This study investigated net anthropogenic nitrogen inputs (NANI, including atmospheric nitrogen deposition, nitrogenous fertilizer use, net nitrogen import in food and feed, and agricultural nitrogen fixation) and the associated relationship with riverine dissolved inorganic nitrogen (DIN) export in the Yangtze River basin during the 1980-2012 period. The total NANI in the Yangtze River basin has increased by more than twofold over the past three decades (3537.0 ± 615.3 to 8176.6 ± 1442.1 kg N km-2 yr-1). The application of chemical fertilizer was the largest component of NANI in the basin (51.1%), followed by net nitrogen import in food and feed (26.0%), atmospheric nitrogen deposition (13.2%), and agricultural nitrogen fixation (9.7%). A regression analysis showed that the riverine DIN export was strongly correlated with NANI and the annual water discharge (R2 = 0.90, p < 0.01). NANI in the Yangtze River basin was estimated to contribute 37-66% to the riverine DIN export. We also forecasted future variations in NANI and riverine DIN export for the years 2013 to 2030, based on possible future changes in human activities and the climate. This work provides a quantitative understanding of NANI in the Yangtze River basin and its effects on riverine DIN export and helps to develop integrated watershed nitrogen management strategies.

  18. Tools for Iina (Life): the journey of the Iina curriculum to the glittering world.

    PubMed

    Arviso, Vivian; Welle, Dorinda; Todacheene, GloJean; Chee, Janet Slowman; Hale-Showalter, Gloria; Waterhouse, Shirley; John, Susie

    2012-01-01

    This article presents the participatory curriculum development process and foundational Diné (Navajo) concepts that inform the Tools for Iina (Life) curriculum, designed for grades 4-6 by a group of Diné educators to strengthen resiliency by addressing children's health, relationships, identity, and sense of the future, utilizing core concepts from Diné oral tradition. Rather than develop a curriculum relying only on experts, and rather than utilize existing American Indian curricula addressing specific risk behaviors or diseases, we facilitated a dialogue with a range of community members to identify core concepts from Diné oral tradition that could provide young people with a perspective on life and its conflicts and challenges, tools for building respectful and supportive relationships, and stories to inform their sense of themselves, the Diné People, and their shared future. The Ways of Life: Iina Project will make the curriculum available in 2012. We offer reflections for other tribes interested in adopting a similar curriculum development process. PMID:22569728

  19. A trans-acting locus regulates an anti-viral expression network and type 1 diabetes risk

    PubMed Central

    Heinig, Matthias; Petretto, Enrico; Wallace, Chris; Bottolo, Leonardo; Rotival, Maxime; Lu, Han; Li, Yoyo; Sarwar, Rizwan; Langley, Sarah R.; Bauerfeind, Anja; Hummel, Oliver; Lee, Young-Ae; Paskas, Svetlana; Rintisch, Carola; Saar, Kathrin; Cooper, Jason; Buchan, Rachel; Gray, Elizabeth E.; Cyster, Jason G.; Erdmann, Jeanette; Hengstenberg, Christian; Maouche, Seraya; Ouwehand, Willem H.; Rice, Catherine M.; Samani, Nilesh J; Schunkert, Heribert; Goodall, Alison H; Schulz, Herbert; Roider, Helge; Vingron, Martin; Blankenberg, Stefan; Münzel, Thomas; Zeller, Tanja; Szymczak, Silke; Ziegler, Andreas; Tiret, Laurence; Smyth, Deborah J.; Pravenec, Michal; Aitman, Timothy J.; Cambien, Francois; Clayton, David; Todd, John A.; Hubner, Norbert; Cook, Stuart A.

    2013-01-01

    Combined analyses of gene networks and DNA sequence variation can provide new insights into the aetiology of common diseases. Here, we used integrated genome-wide approaches across seven rat tissues to identify gene networks and the loci underlying their regulation. We defined an interferon regulatory factor 7 (IRF7)1-driven inflammatory network (iDIN) enriched for viral response genes, which represents a molecular biomarker for macrophages and was regulated in multiple tissues by a locus on rat chromosome 15q25. At this locus, Epstein-Barr virus induced gene 2 (Ebi2 or Gpr183), which we localised to macrophages and is known to control B lymphocyte migration2,3, regulated the iDIN. The human chromosome 13q32 locus, orthologous to rat 15q25, controlled the human equivalent of iDIN, which was conserved in monocytes. For the macrophage-associated autoimmune disease type 1 diabetes (T1D) iDIN genes were more likely to associate with T1D susceptibility than randomly selected immune response genes (P = 8.85 × 10−6). The human locus controlling the iDIN, was associated with the risk of T1D at SNP rs9585056 (P = 7.0 × 10−10, odds ratio = 1.15), which was one of five SNPs in this region associated with EBI2 expression. These data implicate IRF7 network genes and their regulatory locus in the pathogenesis of T1D. PMID:20827270

  20. Modeling increased riverine nitrogen export: Source tracking and integrated watershed-coast management.

    PubMed

    Yu, Dan; Yan, Weijin; Chen, Nengwang; Peng, Benrong; Hong, Huasheng; Zhuo, Guihua

    2015-12-30

    The global NEWS model was calibrated and then used to quantify the long term trend of dissolved inorganic nitrogen (DIN) export from two tributaries of Jiulong River (SE China). Anthropogenic N inputs contributed 61-92% of river DIN yield which increased from 337 in 1980s to 1662 kg N km(-2) yr(-1) in 2000s for the North River, and from 653 to 3097 kg N km(-2) yr(-1) for the West River. North River and West River contributed 55% and 45% respectively of DIN loading to the estuary. Rapid development and poor management driven by national policies were responsible for increasing riverine N export. Scenario analysis and source tracking suggest that reductions of anthropogenic N inputs of at least 30% in the North River (emphasis on fertilizer and manure) and 50% in the West River (emphasis on fertilizer) could significantly improve water quality and mitigate eutrophication in both river and coastal waters. PMID:26517942

  1. A dinoflagellate Cochlodinium geminatum bloom in the Zhujiang (Pearl) River estuary in autumn 2009

    NASA Astrophysics Data System (ADS)

    Ke, Zhixin; Huang, Liangmin; Tan, Yehui; Song, Xingyu

    2012-05-01

    A severe Cochlodinium geminatum red tide (>300 km2) was observed in the Zhujiang (Pearl) River estuary, South China Sea in autumn 2009. We evaluated the environmental conditions and phytoplankton community structure during the outbreak. The red tide water mass had significantly higher dissolved inorganic phosphate (DIP), ammonia, and temperature, but significantly lower nitrite, nitrate, dissolved inorganic nitrogen (DIN), and DIN/DIP relative to the non-red-tide zones. The phytoplankton assemblage was dominated by dinoflagellates and diatoms during the red tide. C. geminatum was the most abundant species, with a peak density of 4.13×107 cell/L, accounting for >65% of the total phytoplankton density. The DIN/DIP ratio was the most important predictor of species, accounting for 12.45% of the total variation in the phytoplankton community. Heavy phosphorus loading, low precipitation, and severe saline intrusion were likely responsible for the bloom of C. geminatum.

  2. Abiotic effects on effluent dissolved organic nitrogen along an estuarine transect.

    PubMed

    Funkey, Carolina P; Latour, Robert J; Bronk, Deborah A

    2015-03-01

    Biological nutrient removal is a process commonly used in water resource recovery facilities to reduce dissolved inorganic nitrogen (DIN) concentrations in effluent; this process is less effective at removing all of the effluent dissolved organic nitrogen (EDON). The goal of this study was to investigate the fate of EDON after it undergoes the disinfection process and enters receiving waters. The authors quantified the abiotic effects of effluent exposure to sunlight, increased salinity, and a combination of the two factors. Effluent dissolved organic nitrogen showed significant breakdown during the disinfection process (UV and chlorine) and when exposed to sunlight and increasing salinity. Approximately 7% of the EDON was transformed to DIN and dissolved primary amines after exposure to 9 hours of sunlight and a salinity increase from 0 to 33. The production of DIN and primary amines should be taken into account when considering sources of labile nitrogen to aquatic ecosystems. PMID:25842537

  3. Responses of phytoplankton community to the input of different aerosols in the East China Sea

    NASA Astrophysics Data System (ADS)

    Meng, X.; Chen, Y.; Wang, B.; Ma, Q. W.; Wang, F. J.

    2016-07-01

    Atmospheric deposition can affect marine phytoplankton by supplying macronutrients and trace elements. We conducted mesocosm experiments by adding aerosols with different composition (dominated by mineral dust, biomass burning and high Cu, and secondary aerosol, respectively) to the surface seawater of the East China Sea. Chlorophyll a concentrations were found to be the highest and lowest after adding aerosols containing the highest Fe and dissolved inorganic nitrogen (DIN), respectively. The relative abundance of Haptophyceae increased significantly after adding mineral dust, whereas diatom, Dinophyceae and Cryptophyceae reached the maximum accompanied with the highest DIN. Our results suggest that Fe may be more important than DIN in promoting primary productivity in the sampled seawater. The input of mineral dust and anthropogenic aerosols may result in distinct changes of phytoplankton community structure.

  4. MULTISLICE SIMULATION OF TRANSMISSION ELECTRON MICROSCOPY IMAGING OF HELIUM BUBBLES IN IRON

    SciTech Connect

    Yao, Bo; Edwards, Danny J.; Kurtz, Richard J.; Odette, George R.; Yamamoto, Takuya

    2011-04-17

    The objective of this task is to establish the size correlation between transmission electron microscopy (TEM) imaged helium (He) bubbles and the actual bubbles in an iron (Fe) matrix. SUMMARY The results of this simulation study show that the size of TEM imaged He bubbles, represented by the inner diameter of the first dark Fresnel ring under defocused condition (Din), deviated from the actual bubble size (Do). Din was found to be larger than Do when imaged with a highly incoherent electron beam, but smaller than Do if the beam is coherent. The deviation of Din from Do increases with increasing defocus. On the other hand, the electron beam accelerating voltage, bubble size, bubble position, and TEM sample thickness do not significantly affect the value of D0/Do. This study also suggests that He bubbles can be differentiated from argon (Ar) bubbles by differences in Fresnel contrast.

  5. The nutrient, total petroleum hydrocarbon and heavy metal contents in the seawater of Bohai Bay, China: Temporal-spatial variations, sources, pollution statuses, and ecological risks.

    PubMed

    Peng, Shitao

    2015-06-15

    Seawater samples collected between 2007 and 2012 were determined the concentrations of nutrient (DIN and DIP), total petroleum hydrocarbon (TPH), and six different heavy metals (As, Cu, Zn, Pb, Cd and Hg). The DIN, DIP, TPH, Pb, and Cd concentrations decreased from 2007 to 2009 or 2010 and increased after 2010. However, the Hg and Cu concentrations increased from 2007 to 2012. In contrast, the As and Zn gradually decreased during the study period. All of the pollutant concentrations gradually decreased from the shoreline to the offshore sites. PCA result showed that urban and port areas, agriculture, and atmospheric deposition were the main sources of pollutants in the bay. Although most of the pollutants were present at concentrations bellow the highest seawater quality standards in China, eutrophication was a risk in Bohai Bay. In addition, DIN was the main pollutant and was responsible for the eutrophication risk in Bohai Bay. PMID:25840871

  6. Modeling and forecasting riverine dissolved inorganic nitrogen export using anthropogenic nitrogen inputs, hydroclimate, and land-use change

    NASA Astrophysics Data System (ADS)

    Huang, Hong; Chen, Dingjiang; Zhang, Baifa; Zeng, Lingzao; Dahlgren, Randy A.

    2014-09-01

    A quantitative understanding of riverine nitrogen (N) export in response to human activities and climate change is critical for developing effective watershed N pollution control measures. This study quantified net anthropogenic N inputs (NANI) and riverine dissolved inorganic N (DIN = NO3-N + NH4-N + NO2-N) export for the upper Jiaojiang River catchment in eastern China over the 1980-2010 time period and examined how NANI, hydroclimate, and land-use practices influenced riverine DIN export. Over the 31-yr study period, riverine DIN yield increased by 1.6-fold, which mainly results from a ∼77% increase in NANI and increasing fractional delivery of NANI due to a ∼55% increase in developed land area. An empirical model that utilizes an exponential function of NANI and a power function of combining annual water discharge and developed land area percentage could account for 89% of the variation in annual riverine DIN yields in 1980-2010. Applying this model, annual NANI, catchment storage, and natural background sources were estimated to contribute 57%, 22%, and 21%, respectively, of annual riverine DIN exports on average. Forecasting based on a likely future climate change scenario predicted a 19.6% increase in riverine DIN yield by 2030 due to a 4% increase in annual discharge with no changes in NANI and land-use compared to the 2000-2010 baseline condition. Anthropogenic activities have increased both the N inputs available for export and the fractional export of N inputs, while climate change can further enhance riverine N export. An integrated N management strategy that considers the influence of anthropogenic N inputs, land-use and climate change is required to effectively control N inputs to coastal areas.

  7. Leaching characteristics of selected South African fly ashes: Effect of pH on the release of major and trace species

    SciTech Connect

    Gitari, W.M.; Fatoba, O.O.; Petrik, L.F.; Vadapalli, V.R.K.

    2009-07-01

    Fly ash samples from two South African coal-fired power stations were subjected to different leaching tests under alkaline and acidic conditions in an attempt to assess the effect of pH on the leachability of species from the fly ashes and also assess the potential impact of the fly ashes disposal on groundwater and the receiving environment. To achieve this, German Standard leaching (DIN-S4) and Acid Neutralization Capacity (ANC) tests were employed. Ca, Mg, Na, K and SO{sub 4} were significantly leached into solution under the two leaching conditions with the total amounts in ANC leachates higher than that of DIN-S4. This indicates that a large fraction of the soluble salts in unweathered fly ash are easily leached. These species represents the fraction that can be flushed off initially from the surface of ash particles on contacting the ash with water. The amounts of toxic trace elements such as As, Se, Cd, Cr and Pb leached out of the fly ashes when in contact with de-mineralized water (DIN-S4 test) were low and below the Target Water Quality Range (TWQR) of South Africa. This is explained by their low concentrations in the fly ashes and their solubility dependence on the pH of the leaching solution. However the amounts of some minor elements such as B, Mn, Fe, As and Se leached out at lower pH ranging between 10 to 4 (ANC test) were slightly higher than the TWQR, an indication that the pH of the leaching solution plays a significant role on the leaching of species in fly ash. The high concentrations of the toxic elements released from the fly ashes at lower pH gives an indication that the disposal of the fly ash could have adverse effects on the receiving environment if the pH of the solution contacting the ashes is not properly monitored.

  8. Characteristics of nutrients in the Jiulong River and its impact on Xiamen Water, China

    NASA Astrophysics Data System (ADS)

    Chen, Baohong; Ji, Weidong; Chen, Jinmin; Lin, Cai; Huang, Haining; Huo, Yunlong; Ji, Xianbiao

    2013-09-01

    Water samples were collected at 20 sites on 4 occasions in 2009 (twice in May, and once in both August and November) along the Jiulong River, South China to examine how nutrient inputs from the Jiulong River could affect the nutrient status of the Xiamen Water. Samples were analyzed for nitrite (NO2-N), nitrate (NO3-N), ammonium (NH4-N), phosphate (PO4-P), silicate (SiO3-Si), salinity, and temperature, to determine the nutrient and trophic status of the river. The results indicate that nutrients are derived mainly from river runoff. NO3-N was the main form of DIN in most parts of the river. In addition, NO3-N, DIN, and SiO3-Si behave conservatively. There is a surplus of DIN and SiO3-Si in the river, and PO4-P is a limitation on phytoplankton growth. The concentration of DIN is typically above 0.60 mg/dm3, and higher than 1.00 mg/dm3 in most parts of the river. The concentration of PO4-P is typically above 0.02 mg/dm3, while the concentration of SiO3-Si is higher than 1.00 mg/dm3. Between 2003 and 2008, samples were collected 3 times per year (May, August and November) at 27 sites in the Xiamen Water and analyzed for NO2-N, NO3-N, NH4-N, PO4-P, salinity, and temperature. We discovered that the Jiulong River was the key source of DIN into the Xiamen Water, but not PO4-P, indicating the reason of the N/P molar ratio imbalance in the Xiamen Water. In the future, the effects of high DIN concentrations on the phytoplankton communities and marine ecosystems of the Xiamen Water shall be studied.

  9. Dissolved organic nitrogen budgets for upland, forested ecosystems in New England

    USGS Publications Warehouse

    Campbell, J.L.; Hornbeck, J.W.; McDowell, W.H.; Buso, D.C.; Shanley, J.B.; Likens, G.E.

    2000-01-01

    Relatively high deposition of nitrogen (N) in the northeastern United States has caused concern because sites could become N saturated. In the past, mass-balance studies have been used to monitor the N status of sites and to investigate the impact of increased N deposition. Typically, these efforts have focused on dissolved inorganic forms of N (DIN = NH4-N + NO3-N) and have largely ignored dissolved organic nitrogen (DON) due to difficulties in its analysis. Recent advances in the measurement of total dissolved nitrogen (TDN) have facilitated measurement of DON as the residual of TDN - DIN. We calculated DON and DIN budgets using data on precipitation and streamwater chemistry collected from 9 forested watersheds at 4 sites in New England. TDN in precipitation was composed primarily of DIN. Net retention of TDN ranged from 62 to 89% (4.7 to 10 kg ha-1 yr-1) of annual inputs. DON made up the majority of TDN in stream exports, suggesting that inclusion of DON is critical to assessing N dynamics even in areas with large anthropogenic inputs of DIN. Despite the dominance of DON in streamwater, precipitation inputs of DON were approximately equal to outputs. DON concentrations in streamwater did not appear significantly influenced by seasonal biological controls, but did increase with discharge on some watersheds. Streamwater NO3-N was the only fraction of N that exhibited a seasonal pattern, with concentrations increasing during the winter months and peaking during snowmelt runoff. Concentrations of NO3-N varied considerably among watersheds and are related to DOC:DON ratios in streamwater. Annual DIN exports were negatively correlated with streamwater DOC:DON ratios, indicating that these ratios might be a useful index of N status of upland forests.

  10. Seasonal dissolved inorganic nitrogen and phosphorus budgets for two sub-tropical estuaries in south Florida, USA

    NASA Astrophysics Data System (ADS)

    Buzzelli, C.; Wan, Y.; Doering, P. H.; Boyer, J. N.

    2013-10-01

    Interactions among geomorphology, circulation, and biogeochemical cycling determine estuary responses to external nutrient loading. In order to better manage watershed nutrient inputs, the goal of this study was to develop seasonal dissolved inorganic nitrogen (DIN) and phosphorus (DIP) budgets for the two estuaries in south Florida, the Caloosahatchee River estuary (CRE) and the St. Lucie Estuary (SLE), from 2002 to 2008. The Land-Ocean Interactions in the Coastal Zone (LOICZ) approach was used to generate water, salt, and DIN and DIP budgets. Results suggested that internal DIN production increases with increased DIN loading to the CRE in the wet season. There were hydrodynamic effects as water column concentrations and ecosystem nutrient processing stabilized in both estuaries as flushing time increased to >10 d. The CRE demonstrated heterotrophy (net ecosystem metabolism or NEM < 0.0) across all wet and dry season budgets. While the SLE was sensitive to DIN loading, system autotrophy (NEM > 0.0) increased significantly with external DIP loading. This included DIP consumption and a bloom of a cyanobacterium (Microcystis aeruginosa) following hurricane-induced discharge to the SLE in 2005. Additionally, while denitrification provided a microbially-mediated N loss pathway for the CRE, this potential was not evident for the SLE where N2 fixation was favored. Disparities between total and inorganic loading ratios suggested that the role of dissolved organic nitrogen (DON) should be assessed for both estuaries. Nutrient budgets indicated that net internal production or consumption of DIN and DIP fluctuated with inter- and intra-annual variations in freshwater inflow, hydrodynamic flushing, and primary production. The results of this study should be included in watershed management plans in order to maintain favorable conditions of external loading relative to internal material cycling in both dry and wet seasons.

  11. Photoaffinity labeling and photoaffinity cross-linking of phosphofructokinase-1 from Saccharomyces cerevisiae by 8-azidoadeninenucleotides.

    PubMed

    Knoche, M; Mönnich, K; Schäfer, H J; Kopperschläger, G

    2001-01-15

    Phosphofructokinase-1 from Saccharomyces cerevisiae is composed of four alpha- and four beta-subunits, each of them carrying catalytic and regulatory bindings sites for MgATP. In this paper, various photoaffinity labels, such as 8-azidoadenosine 5'-triphosphate, 8-azido-1,N6-ethenoadenosine 5'-triphosphate, and 8-N3-3'(2')-O-biotinyl-8-azidoadenosine 5'-triphosphate have been used to study their interaction with the enzyme in the dark and during irradiation. All nucleotidetriphosphates function as phosphate donor forming fructose 1,6-bisphosphate from fructose 6-phosphate. However, the kinetic analysis revealed distinctly differences between them. Photolabeling causes a decrease in enzyme activity to a similar extent, and ATP acts as competitive effector to inactivation. Three bifunctional diazidodiadeninedinucleotides (8-diN3AP4A, monoepsilon-8-diN3AP4A, and diepsilon-8-diN3AP4A) were applied for studying the spatial arrangement of the nucleotide binding sites. No cross-linking of the subunits was obtained by irradiation of the enzyme with 8-diN3AP4A. Photolabeling with diepsilon-8-diN3AP4A resulted in the formation of two alpha-beta cross-links with different mobilities in the SDS-polyacrylamide gel electrophoresis, while monoepsilon-8-diN3AP4A yielded only one alpha-beta cross-link. Because an interfacial location of the catalytic sites between two subunits is less likely, we suggest that the formation of cross-linked subunits may be the result of specific interactions of the bifunctional photolabels with regulatory sites at the interface of both subunits. PMID:11368011

  12. Export of dissolved organic and inorganic nitrogen and total suspended sediments across an urbanization gradient in four tropical rivers of Venezuela

    NASA Astrophysics Data System (ADS)

    Rasse, R. J.; Perez, T.; Giuliante, A.; Donoso, L.

    2012-12-01

    We determined monthly concentrations of total dissolved nitrogen (TDN), dissolved inorganic nitrogen (DIN), dissolved organic nitrogen (DON) and total suspended sediment (TSS) from 4 Venezuelan watersheds from August 2008 to September 2009. Three of these watersheds are mountainous rivers (Tuy, Neverí and Manzanares) and one is flat (Unare River). The three mountainous rivers vary in the degree of urbanization, with the Tuy hosting Caracas, the largest city in Venezuela. We found an order of magnitude larger TDN concentrations in the Tuy, which is impacted mainly by untreated point sources derived from Caracas metropolitan area. The largest TSS values were observed in the three mountainous rivers (Tuy, Neveri and Manzanares). TDN and TSS concentrations varied seasonally with larger TDN and lower TSS values during the dry season for all rivers. Most of the annual discharge of TDN (92%) and TSS (97%) takes place during the rainy season. Our results suggest that urbanization is the largest contributor affecting the composition and magnitude of TDN, whereas orography and local hydrology control the discharge of both TSS and TDN. We calculated the Water Pollution Level (WPL) for DIN and DON to determine the degree of contamination of these species in the evaluated watersheds. WPL values less than 1 indicate that there is in average enough dilution capacity in the river to assimilate the pollutant, whereas WPL larger than 1 indicate that the pollution assimilation capacity has been surpassed. All our evaluated rivers but the Tuy River show WPL-DIN and WPL-DON values between 0.1 and 0.96. The Tuy River had a WPL-DIN=6.3 and WPL-DON= 7.5. We attribute the Tuy River's large DIN and DON contamination to untreated urban point sources due to the strong correlation between population density and DIN and DON concentration from the evaluated watersheds. Our results suggest that urgent water treatment is required for this watershed to diminish the impact in coastal ecosystems.

  13. Effects of nitrate and phosphate supply on chromophoric and fluorescent dissolved organic matter in the Eastern Tropical North Atlantic: a mesocosm study

    NASA Astrophysics Data System (ADS)

    Loginova, A. N.; Borchard, C.; Meyer, J.; Hauss, H.; Kiko, R.; Engel, A.

    2015-12-01

    In open-ocean regions, as is the Eastern Tropical North Atlantic (ETNA), pelagic production is the main source of dissolved organic matter (DOM) and is affected by dissolved inorganic nitrogen (DIN) and phosphorus (DIP) concentrations. Changes in pelagic production under nutrient amendments were shown to also modify DOM quantity and quality. However, little information is available about the effects of nutrient variability on chromophoric (CDOM) and fluorescent (FDOM) DOM dynamics. Here we present results from two mesocosm experiments ("Varied P" and "Varied N") conducted with a natural plankton community from the ETNA, where the effects of DIP and DIN supply on DOM optical properties were studied. CDOM accumulated proportionally to phytoplankton biomass during the experiments. Spectral slope (S) decreased over time indicating accumulation of high molecular weight DOM. In Varied N, an additional CDOM portion, as a result of bacterial DOM reworking, was determined. It increased the CDOM fraction in DOC proportionally to the supplied DIN. The humic-like FDOM component (Comp.1) was produced by bacteria proportionally to DIN supply. The protein-like FDOM component (Comp.2) was released irrespectively to phytoplankton or bacterial biomass, but depended on DIP and DIN concentrations. Under high DIN supply, Comp.2 was removed by bacterial reworking, leading to an accumulation of humic-like Comp.1. No influence of nutrient availability on amino acid-like FDOM component in peptide form (Comp.3) was observed. Comp.3 potentially acted as an intermediate product during formation or degradation of Comp.2. Our findings suggest that changes in nutrient concentrations may lead to substantial responses in the quantity and quality of optically active DOM and, therefore, might bias results of the applied in situ optical techniques for an estimation of DOC concentrations in open-ocean regions.

  14. Impacts of Labile Organic Carbon Concentration on Organic and Inorganic Nitrogen Utilization by a Stream Biofilm Bacterial Community

    PubMed Central

    Leff, Laura G.

    2013-01-01

    In aquatic ecosystems, carbon (C) availability strongly influences nitrogen (N) dynamics. One manifestation of this linkage is the importance in the dissolved organic matter (DOM) pool of dissolved organic nitrogen (DON), which can serve as both a C and an N source, yet our knowledge of how specific properties of DOM influence N dynamics are limited. To empirically examine the impact of labile DOM on the responses of bacteria to DON and dissolved inorganic nitrogen (DIN), bacterial abundance and community composition were examined in controlled laboratory microcosms subjected to various combinations of dissolved organic carbon (DOC), DON, and DIN treatments. Bacterial communities that had colonized glass beads incubated in a stream were treated with various glucose concentrations and combinations of inorganic and organic N (derived from algal exudate, bacterial protein, and humic matter). The results revealed a strong influence of C availability on bacterial utilization of DON and DIN, with preferential uptake of DON under low C concentrations. Bacterial DON uptake was affected by the concentration and by its chemical nature (labile versus recalcitrant). Labile organic N sources (algal exudate and bacterial protein) were utilized equally well as DIN as an N source, but this was not the case for the recalcitrant humic matter DON treatment. Clear differences in bacterial community composition among treatments were observed based on terminal restriction fragment length polymorphisms (T-RFLP) of 16S rRNA genes. C, DIN, and DON treatments likely drove changes in bacterial community composition that in turn affected the rates of DON and DIN utilization under various C concentrations. PMID:24038688

  15. Pore water nutrient characteristics and the fluxes across the sediment in the Pearl River estuary and adjacent waters, China

    NASA Astrophysics Data System (ADS)

    Zhang, Ling; Wang, Lu; Yin, Kedong; Lü, Ying; Zhang, Derong; Yang, Yongqiang; Huang, Xiaoping

    2013-11-01

    Spatio-temporal distribution of pore water nutrients and the fluxes at the sediment-water interface (SWI) were investigated to probe into the geochemical behavior of nutrients associated with early diagenesis of organic matter (OM), and to study the accumulation and transformation processes of nutrients at the SWI, as well as to discuss the impact of riverine inputs on nutrients in the Pearl River estuary (PRE) and adjacent offshore areas. Nutrient concentrations decreased from the upper to the lower reaches of the estuary, suggesting that there was a high input of anthropogenic nutrients and the estuary was acting as a nutrient sink. Dissolved inorganic nitrogen (DIN: the sum of NH4-N, NO3-N and NO2-N) concentrations in the water column and the pore water were higher in the estuary than at offshore areas due to the riverine discharge and the high accumulation rate in the estuary. NO3-N concentration was the highest of the three forms of DIN in the overlying water and showed a sharp decrease from the surficial sediment with increasing sediment depth, indicating that there was strong denitrification at the SWI. NH4-N, mainly deriving from the anaerobic degradation of OM, was the main form of DIN in the pore water and increased with depth. Negative NO3-N fluxes (into the sediment) and positive NH4-N fluxes (from the sediment) were commonly observed from incubation experiments, indicating the denitrification occurred at the SWI. DIN flux suggested that the sediment was a sink of DIN in spring, however, the sediment was the source of DIN in summer and winter. Nutrients dominantly diffused out of the sediment, suggesting that the sediment was the source of nutrients in spring at adjacent offshore areas. The fluxes directed that PO4-P mainly diffused into the sediment while SiO4-Si mainly diffused out of the sediment.

  16. Quantification and characterization of dissolved organic nitrogen in wastewater effluents by electrodialysis treatment followed by size-exclusion chromatography with nitrogen detection.

    PubMed

    Chon, Kangmin; Lee, Yunho; Traber, Jacqueline; von Gunten, Urs

    2013-09-15

    Dissolved organic nitrogen (DON) can act as a precursor of nitrogenous disinfection byproducts during oxidative water treatment. Quantification and characterization of DON are still challenging for waters with high concentrations of dissolved inorganic nitrogen (DIN, including ammonia, nitrate and nitrite) relative to total dissolved nitrogen (TDN) due to the cumulative analytical errors of independently measured nitrogen species (i.e., DON = TDN - NO2(-) - NO3(-) - NH4(+)/NH3) and interference of DIN species to TDN quantification. In this study, a novel electrodialysis (ED)-based treatment for selective DIN removal was developed and optimized with respect to type of ion-exchange membrane, sample pH, and ED duration. The optimized ED method was then coupled with size-exclusion chromatography with organic carbon, UV, and nitrogen detection (SEC-OCD-ND) for advanced DON analysis in wastewater effluents. Among the tested ion-exchange membranes, the PC-AR anion- and CMT cation-exchange membranes showed the lowest DOC loss (1-7%) during ED treatment of a wastewater effluent at ambient pH (8.0). A good correlation was found between the decrease of the DIN/TDN ratio and conductivity. Therefore, conductivity has been adopted as a convenient way to determine the optimal duration of the ED treatment. In the pH range of 7.0-8.3, ED treatment of various wastewater effluents with the PC-AR/CMT membranes showed that the relative residual conductivity could be reduced to less than 0.50 (DIN removal >90%; DIN/TDN ratio ≤ 0.60) with lower DOC losses (6%) than the previous dialysis and nanofiltration methods (DOC loss >10%). In addition, the ED method is shorter (0.5 h) than the previous methods (>1-24 h). The relative residual conductivity was further reduced to ≈ 0.20 (DIN removal >95%; DIN/TDN ratio ≤ 0.35) by increasing the ED duration to 0.7 h (DOC loss = 8%) for analysis by SEC-OCD-ND, which provided new information on distribution and ratio of organic carbon and

  17. Moisture-dependent frictional and aerodynamic properties of safflower seeds

    NASA Astrophysics Data System (ADS)

    Kara, M.; Bastaban, S.; Öztürk, I.; Kalkan, F.; Yildiz, C.

    2012-04-01

    The seeds of two safflower cultivars were investigated in order to determine their frictional and aerodynamic properties as a function of moisture content. The coefficients of dynamic friction of cultivars on aluminium, plywood, fibreglass and steel surfaces increased by 87, 56, 78, and 129% for cv. Remzibey-05 seed, and by 91, 31, 71, and 131% for cv. Dinçer seed, respectively, between the initial and final moisture content levels. The terminal velocities of the Remzibey-05 and Dinçer seeds increased by 15 and 11%, respectively, with increase in moisture content between the initial and final levels.

  18. Ten Years Of Subsidence Monitoring With SAR Interferometry And Its Contribution To Risk Management In Aguascalientes, Mexico

    NASA Astrophysics Data System (ADS)

    Esquivel, Ruben; Castaneda, Laura; Taud, Hind; Lira, Jorge

    2013-12-01

    A Study involving GPS and differential SAR interferometry (DInSAR) monitoring is developed to investigate a subsidence phenomenon in Aguascalientes valley and to obtain displacement models concerned with risk management applications and geodetic purposes. DInSAR study with archived Envisat data allows accumulated displacement mapping yearly, and recent TerraSAR-X data implementation provides a more accurate estimation of displacements, which is used for developing models to correct geodetic positions. The maximum subsidence rate calculated is 15 cm/year with a decreasing rate throughout time in some areas.

  19. Changes in Nitrogen to Phosphorus ratio in the Inner Saronikos Gulf (West Aegean Sea) in relation to the operation of the Sewage Treatment Plant of Athens, Greece

    NASA Astrophysics Data System (ADS)

    Psyllidou-Giouranovits, Rosa

    2013-04-01

    In this work we provide an overview of nitrogen (N) to phosphorus (P) ratio in the inner Saronikos gulf as it has changed over the last twenty five (25) years in relation to the sewage discharges from the Sewage Treatment Plant of Athens in Psittalia Island. Saronikos gulf receives effluents from Athens Metropolitan area (population over 5 million). Until 1994, domestic and industrial sewage of Athens was discharged untreated into the surface water layer of Keratsini and Elefsis bay, whereas, after 1994, the sewage of the Athens Metropolitan area were primarily treated in Psitallia Sewage Treatment Plant and discharged in the inner Saronikos Gulf. Additionally, the secondary stage of the Psittalia Sewage Plant operated in the end of 2004 affecting the nitrogen to phosphorus (DIN:P) ratio (DIN stands for nitrate+nitrite+ammonium). The treated effluent plume frorm Psittalia Sewage Treatment Plant is trapped within the seasonal pycnocline developed during May-November, whereas, during the mixing period (December-April) it reaches the sea-surface. During the last 25 years, significant temporal variation of nutrient concentrations has been observed which has revealed an increase of the DIN:P ratio near the Psittalia Sewage Treatment Plant. In the vicinity of the sewage outfall in Psittalia, DIN:P ratio in the deep layer (30m-bottom) did not show significant variation between the two periods: before and after the operation of the Sewage Treatment Plant (12.9 before the operation of the sewage treatment and 13.3 after the operation of the sewage treatment) showing that inorganic nitrogen and phosphate changed almost with the same rate. However, the limiting factor for phytoplankton growth remains nitrogen. On the contrary, significant increase of DIN:P ratio was observed in the surface layer between the two periods, during summer (stratified period). DIN:P increased from 5.9 for the period 1987-1995 (before the Sewage Treatment Plant operation) to 19.6 for the period 1995

  20. Mechanisms of basin-scale nitrogen load reductions under intensified irrigated agriculture.

    PubMed

    Törnqvist, Rebecka; Jarsjö, Jerker; Thorslund, Josefin; Rao, P Suresh C; Basu, Nandita B; Destouni, Georgia

    2015-01-01

    Irrigated agriculture can modify the cycling and transport of nitrogen (N), due to associated water diversions, water losses, and changes in transport flow-paths. We investigate dominant processes behind observed long-term changes in dissolved inorganic nitrogen (DIN) concentrations and loads of the extensive (465,000 km2) semi-arid Amu Darya River basin (ADRB) in Central Asia. We specifically considered a 40-year period (1960-2000) of large irrigation expansion, reduced river water flows, increased fertilizer application and net increase of N input into the soil-water system. Results showed that observed decreases in riverine DIN concentration near the Aral Sea outlet of ADRB primarily were due to increased recirculation of irrigation water, which extends the flow-path lengths and enhances N attenuation. The observed DIN concentrations matched a developed analytical relation between concentration attenuation and recirculation ratio, showing that a fourfold increase in basin-scale recirculation can increase DIN attenuation from 85 to 99%. Such effects have previously only been observed at small scales, in laboratory experiments and at individual agricultural plots. These results imply that increased recirculation can have contributed to observed increases in N attenuation in agriculturally dominated drainage basins in different parts of the world. Additionally, it can be important for basin scale attenuation of other pollutants, including phosphorous, metals and organic matter. A six-fold lower DIN export from ADRB during the period 1981-2000, compared to the period 1960-1980, was due to the combined result of drastic river flow reduction of almost 70%, and decreased DIN concentrations at the basin outlet. Several arid and semi-arid regions around the world are projected to undergo similar reductions in discharge as the ADRB due to climate change and agricultural intensification, and may therefore undergo comparable shifts in DIN export as shown here for the ADRB

  1. Inter-annual variability of dissolved inorganic nitrogen in the Biobío River, Central Chile: an analysis base on a decadal database along with 1-D reactive transport modeling

    NASA Astrophysics Data System (ADS)

    Yévenes, M.; Figueroa, R.; Parra, O.; Farías, L.

    2015-01-01

    Rivers may act as important sinks (filters) or sources for inorganic nutrients between the land and the sea, depending on the biogeochemical processes and nutrient inputs along the river. This study examines the inter-annual variability of dissolved inorganic nitrogen (DIN) seasonal (wet-dry) cycle for the Biobío River, one of the largest and most industrialized rivers of Central Chile (36°45'-38°49' S and 71°00'-73°20' W). Long-term water flow (1990-2012) and water quality datasets (2004-2012) were used along with a one-dimensional reactive transport ecosystem model to evaluate the effects of water flow and N inputs on seasonal pattern of DIN. From 2004 to 2012, annual average nitrate levels significantly increased from 1.73 ± 2.17 μmol L-1 (upstream of the river) to 18.4 ± 12.7 μmol L-1 (in the river mouth); while the annual average oxygen concentration decreased from 348 ± 22 to 278 ± 42 μmol L-1 between upstream and downstream, indicating an additional oxygen consumption. Variability in the mid-section of the river (station BB8) was identified as a major influence on the inter-annual variability and appeared to be the site of a major anthropogenic disturbance. However, there was also an influence of climate on riverine DIN concentrations; high DIN production occurred during wet years, whereas high consumption proceeded during dry years. Extremely reduced river flow and drought during summer also strongly affected the annual DIN concentration, reducing the DIN production. Additionally, summer storm events during drought periods appeared to cause significant runoff resulting in nitrate inputs to the river. The total DIN input reaching the river mouth was 0.159 Gmol yr-1, implying that internal production exceeds consumption processes, and identifying nitrification as one of the predominant processes occurring in the estuary. In the following, the impact on the river of DIN increases as a nutrient source, as well as climate and biogeochemical factors

  2. Macroalgae δ15N values in well-mixed estuaries: Indicator of anthropogenic nitrogen input or macroalgae metabolism?

    NASA Astrophysics Data System (ADS)

    Raimonet, Mélanie; Guillou, Gaël; Mornet, Françoise; Richard, Pierre

    2013-03-01

    Although nitrogen stable isotope ratio (δ15N) in macroalgae is widely used as a bioindicator of anthropogenic nitrogen inputs to the coastal zone, recent studies suggest the possible role of macroalgae metabolism in δ15N variability. Simultaneous determinations of δ15N of dissolved inorganic nitrogen (DIN) along the land-sea continuum, inter-species variability of δ15N and its sensitivity to environmental factors are necessary to confirm the efficiency of macroalgae δ15N in monitoring nitrogen origin in mixed-use watersheds. In this study, δ15N of annual and perennial macroalgae (Ulva sp., Enteromorpha sp., Fucus vesiculosus and Fucus serratus) are compared to δ15N-DIN along the Charente Estuary, after characterizing δ15N of the three main DIN sources (i.e. cultivated area, pasture, sewage treatment plant outlet). During late winter and spring, when human activities produce high DIN inputs, DIN sources exhibit distinct δ15N signals in nitrate (NO) and ammonium (NH): cultivated area (+6.5 ± 0.6‰ and +9.0 ± 11.0‰), pasture (+9.2 ± 1.8‰ and +12.4‰) and sewage treatment plant discharge (+16.9 ± 8.7‰ and +25.4 ± 5.9‰). While sources show distinct δN- in this multiple source catchment, the overall mixture of NO sources - generally >95% DIN - leads to low variations of δN-NO at the mouth of the estuary (+7.7 to +8.4‰). Even if estuarine δN-NO values are not significantly different from pristine continental and oceanic site (+7.3‰ and +7.4‰), macroalgae δ15N values are generally higher at the mouth of the estuary. This highlights high anthropogenic DIN inputs in the estuary, and enhanced contribution of 15N-depleted NH in oceanic waters. Although seasonal variations in δN-NO are low, the same temporal trends in macroalgae δ15N values at estuarine and oceanic sites, and inter-species differences in δ15N values, suggest that macroalgae δ15N values might be modified by the metabolic response of macroalgae to environmental parameters (e

  3. Mechanisms of Basin-Scale Nitrogen Load Reductions under Intensified Irrigated Agriculture

    PubMed Central

    Törnqvist, Rebecka; Jarsjö, Jerker; Thorslund, Josefin; Rao, P. Suresh C.; Basu, Nandita B.; Destouni, Georgia

    2015-01-01

    Irrigated agriculture can modify the cycling and transport of nitrogen (N), due to associated water diversions, water losses, and changes in transport flow-paths. We investigate dominant processes behind observed long-term changes in dissolved inorganic nitrogen (DIN) concentrations and loads of the extensive (465,000 km2) semi-arid Amu Darya River basin (ADRB) in Central Asia. We specifically considered a 40-year period (1960–2000) of large irrigation expansion, reduced river water flows, increased fertilizer application and net increase of N input into the soil-water system. Results showed that observed decreases in riverine DIN concentration near the Aral Sea outlet of ADRB primarily were due to increased recirculation of irrigation water, which extends the flow-path lengths and enhances N attenuation. The observed DIN concentrations matched a developed analytical relation between concentration attenuation and recirculation ratio, showing that a fourfold increase in basin-scale recirculation can increase DIN attenuation from 85 to 99%. Such effects have previously only been observed at small scales, in laboratory experiments and at individual agricultural plots. These results imply that increased recirculation can have contributed to observed increases in N attenuation in agriculturally dominated drainage basins in different parts of the world. Additionally, it can be important for basin scale attenuation of other pollutants, including phosphorous, metals and organic matter. A six-fold lower DIN export from ADRB during the period 1981–2000, compared to the period 1960–1980, was due to the combined result of drastic river flow reduction of almost 70%, and decreased DIN concentrations at the basin outlet. Several arid and semi-arid regions around the world are projected to undergo similar reductions in discharge as the ADRB due to climate change and agricultural intensification, and may therefore undergo comparable shifts in DIN export as shown here for the

  4. Freshwater and Saline Loads of Dissolved Inorganic Nitrogen to Hood Canal and Lynch Cove, Western Washington

    USGS Publications Warehouse

    Paulson, Anthony J.; Konrad, Christopher P.; Frans, Lonna M.; Noble, Marlene; Kendall, Carol; Josberger, Edward G.; Huffman, Raegan L.; Olsen, Theresa D.

    2006-01-01

    Hood Canal is a long (110 kilometers), deep (175 meters) and narrow (2 to 4 kilometers wide) fjord of Puget Sound in western Washington. The stratification of a less dense, fresh upper layer of the water column causes the cold, saltier lower layer of the water column to be isolated from the atmosphere in the late summer and autumn, which limits reaeration of the lower layer. In the upper layer of Hood Canal, the production of organic matter that settles and consumes dissolved oxygen in the lower layer appears to be limited by the load of dissolved inorganic nitrogen (DIN): nitrate, nitrite, and ammonia. Freshwater and saline loads of DIN to Hood Canal were estimated from available historical data. The freshwater load of DIN to the upper layer of Hood Canal, which could be taken up by phytoplankton, came mostly from surface and ground water from subbasins, which accounts for 92 percent of total load of DIN to the upper layer of Hood Canal. Although DIN in rain falling on land surfaces amounts to about one-half of the DIN entering Hood Canal from subbasins, rain falling directly on the surface of marine waters contributed only 4 percent of the load to the upper layer. Point-source discharges and subsurface flow from shallow shoreline septic systems contributed less than 4 percent of the DIN load to the upper layer. DIN in saline water flowing over the sill into Hood Canal from Admiralty Inlet was at least 17 times the total load to the upper layer of Hood Canal. In September and October 2004, field data were collected to estimate DIN loads to Lynch Cove - the most inland marine waters of Hood Canal that routinely contain low dissolved-oxygen waters. Based on measured streamflow and DIN concentrations, surface discharge was estimated to have contributed about one-fourth of DIN loads to the upper layer of Lynch Cove. Ground-water flow from subbasins was estimated to have contributed about one-half of total DIN loads to the upper layer. In autumn 2004, the relative

  5. Late Variscan postcollisional extensional events in the Danubian domain (Romania): the Motru Dyke System

    NASA Astrophysics Data System (ADS)

    Campeanu, Mara; Balica, Constantin; Balintoni, Ioan; Stremtan, Cosmin

    2013-04-01

    trace elements concentrations as compared to the felsic ones, even though their trends are similar. The high values of Al concentrations (exceeding 14%) correlated to the Na concentrations and the absence of Eu anomalies in the REE distributions, exclude the plagioclase fractionation in the bulk generation of these melts. Sr and Nd isotopic data from several components of the MDS, do not comply with the previous assumptions regarding source, yet indicates a heterogeneous source of mixed mantle and dominant crustal origin. The negative values of initial Nd (assuming an age of 300 Ma), for 12 out of 13 collected samples ranging from - 13.0 to + 5.7 (with clustering between -6 and 0) and the relatively high 87Sr/86Sr initial ratios (0.70745-0.73746) point clearly to a mixing of sub-crustal and crustal derived melts. Trace elements concentrations along with the radiogenic isotope data support the assumption of an enriched mixed mantle source with crustal components that is also sustained by the presence of inherited zircon grains in MDS. Therefore we can constrain the emplacement age of MDS to Late Paleozoic (i.e. Carboniferous). It is very likely that they might have been generated during the post-collisional late Variscan extensional event, in a very likely relation to lower crustal delamination that might have triggered the partial melting of the uppermost mantle, which could have induced the partial melting of crustal components. [1] Balintoni, I., Balica, C., 2012. Avalonian, Ganderian and East Cadomian terranes in South Carpathians, Romania, and Pan-African events recorded in their basement. Mineralogy and Petrology - DOI: 10.1007/s00710-012-0206-x [2] Berza, T., Seghedi, A., 1975. The pre-Silurian filonian complex from Motru Basin (in Romanian) Dări de Seamă ale Institutului de Geologie şi Geofizică, LXI/1, 131-149. [3] Féménias, O., Berza, T., Tatu, M., Diot, H., Demaiffe, D., 2008. Nature and signifiance of a Cambro-Ordovician high-K, calc-alkaline sub

  6. Evaluation of the catalytic reduction of nitrate for the determination of dissolved organic nitrogen in natural waters.

    PubMed

    Ambonguilat, Sonia; Gallard, Hervé; Garron, Anthony; Epron, Florence; Croué, Jean Philippe

    2006-02-01

    The catalytic reduction of nitrate ions into nitrogen gas was tested to partly remove dissolved inorganic nitrogen (DIN) before the determination of dissolved organic nitrogen (DON). Experiments were conducted on nitrate solutions enriched with natural organic matter (NOM) isolates previously extracted from surface waters. Three catalysts Pd-In/Al2O3, Pd-Sn/Al2O3 and Pd/SnO2 were tested. Their noble metal (palladium) and promoter metal (indium or tin) contents are 5 and 1.75 wt%, respectively. Preliminary experiments performed on a solution containing 17 amino acids showed that most compounds were removed by less than 15%, probably due to sorption onto the catalysts. Reduction of nitrate in absence of NOM was complete after 20 min of reaction time and the removal of DIN was about 80% (about 19% formation of ammonium). In the presence of NOM (DOC 20 mgC/L, DON 0.67 mg N/L), the kinetic of nitrate reduction was slower and the reduction in DIN content was limited to 15% i.e. selectivity toward ammonium reached 85%. Adsorption tests showed a similar removal of both DOC and DON of about 70% and 30% onto Pd-Sn/Al2O3 and Pd/SnO2 catalysts, respectively, which confirmed that NOM probably compete with nitrate for active catalytic sites. In conclusion, catalytic reduction of nitrate before DON determination cannot be used because of DON sorption and low DIN removal. PMID:16436291

  7. Managing & Re-Using Didactical Expertise: The Didactical Object Model

    ERIC Educational Resources Information Center

    Pawlowski, Jan M.; Bick, Markus

    2006-01-01

    The DIN Didactical Object Model extends the approaches of existing Educational Modeling Languages introducing specifications for contexts and experiences. In this paper, we show how the Didactical Object Model can be used for sharing didactical expertise. Educational Modeling Languages change the design paradigm from content orientation towards…

  8. Beneath the Surface.

    ERIC Educational Resources Information Center

    Cohen, Andrew

    1998-01-01

    Discusses wood-flooring components for athletic facilities, what materials make up these floors, and how they affect a floor's performance once installed. The pros and cons of anchored versus floating systems are examined, including the issue of moisture resistance. Also discussed is the influence of Deutsches Institut fur Normans (DIN) standards…

  9. THE RELATIVE IMPORTANCE OF NUTRIENT ENRICHMENT AND HERBIVORY ON MACROALGAL COMMUNITIES NEAR NORMAN'S POND CAY, EXUMAS CAYS, BAHAMAS: A "NATURAL" ENRICHMENT EXPERIMENT. (R830414)

    EPA Science Inventory

    The simultaneous effects of grazing and nutrient enrichment on macroalgal communities were experimentally investigated using plastic mesh enclosure/exclosure cages along a natural nutrient (DIN, SRP) gradient from the discharge of a tidal mangrove creek on the west side of Nor...

  10. Model-aided quantification of dissolved carbon and nitrogen release after windthrow disturbance in an Austrian karst system

    NASA Astrophysics Data System (ADS)

    Hartmann, A.; Kobler, J.; Kralik, M.; Dirnböck, T.; Humer, F.; Weiler, M.

    2016-01-01

    Karst systems are important for drinking water supply. Future climate projections indicate increasing temperature and a higher frequency of strong weather events. Both will influence the availability and quality of water provided from karst regions. Forest disturbances such as windthrow can disrupt ecosystem cycles and cause pronounced nutrient losses from the ecosystems. In this study, we consider the time period before and after the wind disturbance period (2007/08) to identify impacts on DIN (dissolved inorganic nitrogen) and DOC (dissolved organic carbon) with a process-based flow and solute transport simulation model. When calibrated and validated before the disturbance, the model disregards the forest disturbance and its consequences on DIN and DOC production and leaching. It can therefore be used as a baseline for the undisturbed system and as a tool for the quantification of additional nutrient production. Our results indicate that the forest disturbance by windthrow results in a significant increase of DIN production lasting ˜ 3.7 years and exceeding the pre-disturbance average by 2.7 kg ha-1 a-1 corresponding to an increase of 53 %. There were no significant changes in DOC concentrations. With simulated transit time distributions we show that the impact on DIN travels through the hydrological system within some months. However, a small fraction of the system outflow (< 5 %) exceeds mean transit times of > 1 year.

  11. In the Social Register: Pronoun Choice in Norwegian and English.

    ERIC Educational Resources Information Center

    Mills, Carl

    Choice of second-person pronouns can help explain the intersection of language, personality, and culture. Changes in modern Norway are described in which the polite forms "de,""dem"/"dykk," and "deres"/"dykkar" have been replaced in all except commercial, government, or ultra-polite speech by the familiar terms "du,""deg"/"dae," and "din." Some…

  12. WATER QUALITY MODELING IN THE RIO CHONE ESTUARY

    EPA Science Inventory

    Water quality in the Rio Chone Estuary, a seasonally inverse, tropical estuary, in Ecuador was characterized by modeling the distribution of biochemical oxygen demand (BOD) and dissolved inorganic nitrogen (DIN) within the water column. These two variables are modeled using modif...

  13. A Connector between Active Laboratory Probes and Computer Interfaces of Different Brands

    ERIC Educational Resources Information Center

    Hui, Kaleonui J.; Coney, John P.; Binder, P.-M.

    2016-01-01

    In PASCO's Technical Article #763, it is stated that "PASCO [also] makes sensors with an 8-pin DIN connector. These sensors cannot be used with the Vernier interfaces, as they have different power requirements." Recently we had the need to make such connections in a 10-station freshman lab. Since the Vernier input is a 6-way BT 631A plug…

  14. Legacy of German psychiatric genetics: hindsight is always 20/20.

    PubMed

    Gottesman, I I; Bertelsen, A

    1996-07-26

    The history of psychiatric genetics is informed by this paper, which serves to review the legacy of German psychiatric genetics and its antecedents during the twentieth century. It also serves as an introduction to two new annotated abstracts of basic research papers on family studies of schizophrenia by Ernst Rüdin in 1916 and by Bruno Schulz in 1932, submitted by Kenneth Kendler and Edith Zerbin-Rüdin, together with another paper by them describing the origin and activities of Rüdin's Munich School of Psychiatric Genetics (1917-1945). Our paper also introduces an invited critical summary of the work of Ernst Rüdin by his biographer Matthias M. Weber, a medical historian working in the Historical Archives of the Clinical Institute of the Max Planck Institute of Psychiatry in Munich. We raise a number of bioethical questions in the context of the uses and misuses made of genetic information in the service of the Nazi programs of eugenics, "euthanasia," and genocide. PMID:8837696

  15. Factors influencing export of dissolved inorganic nitrogen by major rivers: a new seasonal, spatially explicit, global model

    EPA Science Inventory

    Background/Question/Methods Substantial effort has focused on understanding spatial variation in dissolved inorganic nitrogen (DIN) export to the coastal zone and specific basins have been studied in some depth. Much less is known, however, about seasonal patterns and controls ...

  16. Education in Spotlight on Statewide Ballots

    ERIC Educational Resources Information Center

    Ash, Katie

    2008-01-01

    Education issues are poised to break through the din of presidential politics and economic anxiety in more than a dozen states next month, as voters confront ballot questions and constitutional amendments involving K-12 policy and school finance. High on the list are gambling referendums in six states--Arkansas, Colorado, Maine, Maryland,…

  17. Computational Insights into the Different Resistance Mechanism of Imidacloprid versus Dinotefuran in Bemisia tabaci.

    PubMed

    Meng, Xiaoqing; Zhu, Chengchun; Feng, Yue; Li, Weihua; Shao, Xusheng; Xu, Zhiping; Cheng, Jiagao; Li, Zhong

    2016-02-17

    Insecticide resistance is a critical problem for pest control and management. For Bemisia tabaci, striking high metabolic resistance (generally conferred by CYP6CM1) was observed for imidacloprid (IMI) and most other neonicotinoid members. However, dinotefuran (DIN) displayed very low resistance factors, which indicated distinct metabolic properties. Here, molecular modeling methods were applied to explore the different resistance features of IMI versus DIN within the Q type of CYP6CM1. It was found that Arg225 played crucial roles in the binding of IMI-CYP6CM1vQ with a cation-π interaction and two stable H-bonds; however, such interactions were all absent in the DIN-CYP6CM1vQ system. The stable binding of IMI with CYP6CM1vQ would facilitate the following metabolic reaction, while the weak binding of DIN might disable its potential metabolism, which should be an important factor for their distinct resistance levels. The findings might facilitate future design of the antiresistance neonicotinoid molecules. PMID:26817991

  18. The low-affinity ATP binding site of the Escherichia coli SecA dimer is localized at the subunit interface.

    PubMed

    van der Wolk, J P; Boorsma, A; Knoche, M; Schäfer, H J; Driessen, A J

    1997-12-01

    The homodimeric SecA protein is the ATP-dependent force generator in the Escherichia coli precursor protein translocation cascade. SecA contains two essential nucleotide binding sites (NBSs), i.e., NBS1 and NBS2 that bind ATP with high and low affinity, respectively. The photoactivatable bifunctional cross-linking agent 3'-arylazido-8-azidoadenosine 5'-triphosphate (diN3ATP) was used to investigate the spatial arrangement of the nucleotide binding sites of SecA. DiN3ATP is an authentic ATP analogue as it supports SecA-dependent precursor protein translocation and translocation ATPase. UV-induced photo-cross-linking of the diN3ATP-bound SecA results in the formation of stable dimeric species of SecA. D209N SecA, a mutant unable to bind nucleotides at NBS1, was also photo-cross-linked by diN3ATP, whereas no cross-linking occurred with the NBS2 mutant R509K SecA. We concluded that the low-affinity NBS2, which is located in the carboxyl-terminal half of SecA, is the site of crosslinking and that NBS2 binds nucleotides at or near the subunit interface of the SecA dimer. PMID:9398216

  19. Challenging Texts: Teaching Deliberately--Reading Henry David Thoreau's "Walden"

    ERIC Educational Resources Information Center

    Davis, Andrea

    2011-01-01

    One of the more difficult 19th-century American texts for high school students to read is undoubtedly Henry David Thoreau's "Walden." His erudite allusions, often page-long sentences, and sophisticated sense of the ironic initially leave many students cold. Still, the author encourages them to read amid the din of a cultural cacophony that shouts…

  20. THE DEVELOPMENT OF IODINE BASED IMPINGER SOLUTIONS FOR THE EFFICIENT CAPTURE OF HG USING DIRECT INJECTION NEBULIZATION - INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY ANALYSIS

    EPA Science Inventory

    Inductively coupled plasma mass spectrometry (ICP/MS) with direct injection nebulization (DIN) was used to evaluate novel impinger solution compositions capable of capturing elemental mercury (Hgo) in EPA Method 5 type sampling. An iodine based impinger solutoin proved to be ver...

  1. PHOSPHORUS-RICH WATERS AT GLOVERS REEF, BELIZE? (R830414)

    EPA Science Inventory

    Table 1. Concentrations (small mu, GreekM) of ammonium, nitrate, dissolved inorganic nitrogen (DIN=ammonium + nitrate + nitrite), PO43−–P (...

  2. INPUT-OUTPUT BUDGETS OF INORGANIC NITROGEN FOR 24 FOREST WATERSHEDS IN THE NORTHEASTERN UNITED STATES: A REVIEW

    EPA Science Inventory

    Input-output budgets for dissolved inorganic nitrogen (DIN) are summarized for 24 small watersheds at 15 locations in the northeasternUnited States. The study watersheds are completely forested, free of recent physical disturbances, and span a geographical region bounded by West ...

  3. CAPILLARY ELECTROPHORESIS COUPLED ON-LINE WITH INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY FOR ELEMENTAL SPECIATION

    EPA Science Inventory

    A novel interface to connect a capillary electrophoresis (CE) system with an inductively coupled plasma mass spectrometric (ICPMS) detector is reported here. The interface was built using a direct injection nebulizer (DIN) system. In this interface, the CE capillary was placed co...

  4. Towards ecologically relevant targets for river pollutant loads to the Great Barrier Reef.

    PubMed

    Kroon, Frederieke J

    2012-01-01

    Degradation of coastal ecosystems in the Great Barrier Reef (GBR), Australia, has been linked with a decline in water quality from land-based runoff. This paper examines the reduction in current end-of-catchment loads required for total suspended solids (TSS) and dissolved inorganic nitrogen (DIN) to achieve GBR water quality guidelines. Based on first-order estimates of sustainable pollutant loads, current TSS and DIN loads would need to be reduced by approximately 7000ktons/y (41%) and 6000tons/y (38%), respectively. Next, these estimated reductions for TSS and DIN are compared with Reef Plan targets for anthropogenic sediment (-20% by 2020) and nitrogen (-50% by 2013) loads. If successful, these targets will accomplish approximately 40% of TSS and 92% of DIN load reductions required to achieve sustainable loads to the GBR lagoon. These first-order estimates elucidate the need to establish ecologically relevant targets for river pollutant loads to the GBR for management and policy. PMID:22136764

  5. Phase selection during laser surface melting of martensitic stainless tool steels

    SciTech Connect

    Colaco, R.; Vilar, R.

    1997-01-15

    Laser surface melting (LSM) of tool steels allows for the complete dissolution of large brittle carbides, leading to homogeneous and extremely fine microstructures. Due to its characteristics, LSM allows improvement of the performance of tool steels by increasing their resistance to erosive and abrasive wear. Nevertheless, when DIN X42Cr13 and DIN X100Cr18 martensitic stainless steels are submitted to LSM, considerable amounts of austenite and {delta}-ferrite formed during the first stage of solidification can be retained in metastable condition at room temperature by mechanisms which are not yet fully understood. The purpose of the present work is to establish the influence of solidification conditions on the primary solidification mode of these two martensitic stainless tool steels, aimed to optimize the LSM operating conditions. Accordingly, samples of DIN X40Cr13 and DIN X100Cr18 were submitted to LSM with a wide range of solidification speeds. The microstructures were analyzed in order to identify the primary solidification mode. The experimental results were compared with theoretical predictions, based on comparison of the dendrite tip temperatures of austenite and {delta}-ferrite as function of the solidification speed.

  6. Monitoring of Land Subsidence in Ravenna Municipality Using Integrated SAR - GPS Techniques: Description and First Results

    NASA Astrophysics Data System (ADS)

    Artese, G.; Fiaschi, S.; Di Martire, D.; Tessitore, S.; Fabris, M.; Achilli, V.; Ahmed, A.; Borgstrom, S.; Calcaterra, D.; Ramondini, M.; Artese, S.; Floris, M.; Menin, A.; Monego, M.; Siniscalchi, V.

    2016-06-01

    The Emilia Romagna Region (N-E Italy) and in particular the Adriatic Sea coastline of Ravenna, is affected by a noticeable subsidence that started in the 1950s, when the exploitation of on and off-shore methane reservoirs began, along with the pumping of groundwater for industrial uses. In such area the current subsidence rate, even if lower than in the past, reaches the -2 cm/y. Over the years, local Authorities have monitored this phenomenon with different techniques: spirit levelling, GPS surveys and, more recently, Differential Interferometric Synthetic Aperture Radar (DInSAR) techniques, confirming the critical situation of land subsidence risk. In this work, we present the comparison between the results obtained with DInSAR and GPS techniques applied to the study of the land subsidence in the Ravenna territory. With regard to the DInSAR, the Small Baseline Subset (SBAS) and the Coherent Pixel Technique (CPT) techniques have been used. Different SAR datasets have been exploited: ERS-1/2, ENVISAT, TerraSAR-X and Sentinel-1. Some GPS campaigns have been also carried out in a subsidence prone area. 3D vertices have been selected very close to existing persistent scatterers in order to link the GPS measurement results to the SAR ones. GPS data were processed into the International reference system and the comparisons between the coordinates, for the first 6 months of the monitoring, provided results with the same trend of the DInSAR data, even if inside the precision of the method.

  7. Mass balances of mercury and nitrogen in burned and unburned forested watersheds at Acadia National Park, Maine, USA

    USGS Publications Warehouse

    Nelson, S.J.; Johnson, K.B.; Kahl, J.S.; Haines, T.A.; Fernandez, I.J.

    2007-01-01

    Precipitation and streamwater samples were collected from 16 November 1999 to 17 November 2000 in two watersheds at Acadia National Park, Maine, and analyzed for mercury (Hg) and dissolved inorganic nitrogen (DIN, nitrate plus ammonium). Cadillac Brook watershed burned in a 1947 fire that destroyed vegetation and soil organic matter. We hypothesized that Hg deposition would be higher at Hadlock Brook (the reference watershed, 10.2 ??g/m2/year) than Cadillac (9.4 ??g/m2/year) because of the greater scavenging efficiency of the softwood vegetation in Hadlock. We also hypothesized the Hg and DIN export from Cadillac Brook would be lower than Hadlock Brook because of elemental volatilization during the fire, along with subsequently lower rates of atmospheric deposition in a watershed with abundant bare soil and bedrock, and regenerating vegetation. Consistent with these hypotheses, Hg export was lower from Cadillac Brook watershed (0.4 ??g/m2/year) than from Hadlock Brook watershed (1.3 ??g/m2/year). DIN export from Cadillac Brook (11.5 eq/ ha/year) was lower than Hadlock Brook (92.5 eq/ha/year). These data show that ??50 years following a wildfire there was lower atmospheric deposition due to changes in forest species composition, lower soil pools, and greater ecosystem retention for both Hg and DIN. ?? Springer Science + Business Media B.V. 2006.

  8. Variability Associated with As in Vivo–in Vitro Correlations When Using Different Bioaccessibility Methodologies

    EPA Science Inventory

    To evaluate the capabilities of in vitro assays to predict arsenic (As) relative bioavailability (RBA), we examined the relationship between As bioaccessibility, determined using a number of in vitro bioaccessibility (IVBA) methodologies (SBRC, IVG, PBET, DIN and UBM) and As RBA ...

  9. REGIONAL ANALYSIS OF INORGANIC NITROGEN YIELD AND RETENTION IN HIGH-ELEVATION ECOSYSTEMS OF THE SIERRA NEVADA AND ROCKY MOUNTAINS

    EPA Science Inventory

    Yields and retention of inorganic nitrogen (DIN) and nitrate concentrations in surface runoff are summarized for 28 high elevation watersheds in the Sierra Nevada, California and Rocky Mountains of Wyoming and Colorado. Catchments ranged in elevation from 2475 to 3603 m and from...

  10. Reimagining Reading: Creating a Classroom Culture That Embraces Independent Choice Reading

    ERIC Educational Resources Information Center

    Dickerson, Katie

    2015-01-01

    Many of us are plagued by negative memories of sustained silent reading. In some of these memories, we are the students, attempting to read a book that didn't hold our interest or trying to read over the din of our disengaged classmates. In other memories, we are the teachers, suffering through a ten-minute classroom management nightmare, deciding…

  11. The Flogging of For-Profit Colleges

    ERIC Educational Resources Information Center

    Hassler, Richard P.

    2006-01-01

    A din of opprobrium rises from the academy against for-profit education. But a name like "The University of Phoenix" is not misleading to students, Richard P. Hassler argues, even though the dividends that that large public corporation pays its shareholders derive narrowly from career preparation, rather than from more rarefied cultivations of the…

  12. Experiences with new European standards for calculation of flanged joints including necessary gasket factors

    SciTech Connect

    Bartonicek, J.; Kockelmann, H.; Schoeckle, F.

    1996-12-01

    The correct function of a flanged joint in a given construction (flanges, bolts) and with given loads (pressure, temperature, additional forces) depends on the following: (1) choice of the gasket for the operation environment; (2) determination of the gasket stress for assembly conditions; and (3) gasket stress in operation, i.e., the conditions when leakage has to be minimized. For the correct choice of a gasket it is necessary to have gasket factors available, as given by DIN 28090 (Sept. 95) together with the test procedures. These gasket factors determine the tightening characteristics as well as the deformation capability of the gaskets. Furthermore, these gasket factors must be available for use in calculations. On the basis of calculations a stress analysis (i.e. a limitation of the stresses in flanges and bolts) must be possible as well as the determination of the bolt forces for the assembly state and of the gasket stress for operating conditions. This is one of the goals of the standard pr DIN EN 1591. The paper first introduces the actual European standards. Calculations are reviewed using test data from special test rigs and from real flanged joints. The calculations were made according to DIN 2505 (initial German standard for flanged joints) and pr DIN EN 1591. Additionally, 2D and 3D Finite Element analysis calculations were performed.

  13. Assessing the potential to decrease the Gulf of Mexico hypoxic zone with Midwest US perennial cellulosic feedstock production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The goal of this research is to determine the changes in streamflow, dissolved inorganic nitrogen (DIN) leaching and export to the Gulf of Mexico associated with a range of large-scale dedicated perennial cellulosic bioenergy production scenarios within in the Mississippi-Atchafalaya River Basin (MA...

  14. Cultural and Technical Evaluation of Heating Alternatives to Improve Indoor Air Quality on the Navajo Nation

    EPA Science Inventory

    In the Navajo Nation it is estimated that 62% of households use wood as their primary means of heating1. A 2010 study by the U.S. Geological Survey (USGS) and Diné College found that in Shiprock, NM, the largest town in the Navajo Nation (pop. = 8,300)2, heating is often w...

  15. Evaluation of a direct injection nebulizer interface for flow injection analysis and high performance liquid chromatography with inductively coupled plasma-atomic emission spectroscopic detection

    SciTech Connect

    LaFreniere, K.E.

    1986-06-01

    A direct injection nebulizer (DIN) was designed, developed, and evaluated to determine its potential utilization as an effective interface for flow injection analysis (FIA) and high performance liquid chromatography (HPLC) coupled with inductively coupled plasma-atomic emission spectroscopic detection. The analytical figures of merit for the DIN when used as an interface for FIA-ICP-AES were found to be comparable to or better than those obtained with conventional pneumatic nebulization in terms of limits of detection (LODs), reproducibility, linearity, and interelement effects. Stable plasma operation was maintained for the DIN sample introduction of a variety of pure organic solvents, including acetonitrile, methanol, methylisobutylketone, and pyridine. The HPLC-DIN-ICP-AES facility was specifically applied for the speciation of inorganic and organometallic species contained in synthetic mixtures, vanilla extracts, and a variety of energy-related materials, such as shale oil process water, coal extracts, shale oil, crude oil, and an SRC II. Suggestions for future research are also considered. 227 refs., 44 figs., 15 tabs.

  16. Evaluation of a direct injection nebulizer interface for flow injection analysis and high performance liquid chromatography with inductively coupled plasma-atomic emission spectroscopic detection

    SciTech Connect

    LaFreniere, K.E.

    1986-01-01

    A direct injection nebulizer (DIN) was designed, developed and evaluated to determine its potential utilization as an effective interface for flow injection analysis (FIA) and high performance liquid chromatography (HPLC) coupled with inductively coupled plasma-atomic emission spectroscopic detection. The analytical figures of merit for the DIN when used as an interface for FIA-ICP-AES were found to be comparable to or better than those obtained with conventional pneumatic nebulization in terms of limits of detection (LODs), reproducibility, linearity, and interelement effects. In the HPLC mode, the LODDs were found to be comparable to those obtained by continuous-flow sample introduction into the ICP, or inferior by up to only a factor of four. Stable plasma operation was maintained for the DIN sample introduction of a variety of pure organic solvents, including acetonitrile, methanol, methyl-isobutylketone, and pyridine. The HPLC-DIN-ICP-AES facility was specifically applied for the speciation of inorganic and organo-metallic species contained in synthetic mixtures, vanilla extracts and a variety of energy-related materials, such as shale oil process water, coal extracts, shale oil, crude oil, and an SRC II. Suggestions for future research are also considered.

  17. Factors influencing export of dissolved inorganic nitrogen by major rivers: A new seasonal, spatially explicit, global model - 2012

    EPA Science Inventory

    Background/Question/Methods Substantial effort has focused on understanding spatial variation in dissolved inorganic nitrogen (DIN) export to the coastal zone and specific basins have been studied in some depth. Much less is known, however, about seasonal patterns and zone and ...

  18. What Paradox? Referential Cues Allow for Infant Use of Phonetic Detail in Word Learning

    ERIC Educational Resources Information Center

    Fennell, Christopher T.; Waxman, Sandra R.

    2010-01-01

    Past research has uncovered a surprising paradox: Although 14-month-olds have exquisite phonetic discrimination skills (e.g., distinguishing [b] from [d]), they have difficulty using phonetic detail when mapping "novel" words to objects in laboratory tasks (confusing "bin" and "din"). While some have attributed infants' difficulty to immature word…

  19. Hydrologic and biotic control of nitrogen export during snowmelt: A combined conservative and reactive tracer approach

    NASA Astrophysics Data System (ADS)

    Petrone, Kevin; Buffam, Ishi; Laudon, Hjalmar

    2007-06-01

    Dissolved inorganic nitrogen (DIN) and dissolved organic nitrogen (DON) stored in the snowpack are important sources of N in snow-covered ecosystems, yet we have limited knowledge of their fate during the melt period. Our objective was to quantify the role of hydrologic and biogeochemical processes in regulating stream fluxes of DIN (NO3- + NH4+) and DON in a forest-dominated and a wetland-dominated catchment during the snowmelt period. We combined isotopic hydrograph separation with concurrent measurements of meltwater DIN and DON to calculate "conservative" N export (hydrologic mixing only) and compared it with "reactive" N export (i.e., observed fluxes that include biogeochemical processes). On balance, N was retained in the catchments during snowmelt because of storage of meltwater N in soils, but our N export comparison revealed N generation (mostly as DON) from the mobilization of dissolved organic matter. In contrast, NO3-, which was highly enriched in snowpack meltwater, remained below detection in streams, and both catchments were sinks for NO3-, suggesting that denitrification and/or uptake may be important at the catchment scale. Over the melt period, the forest catchment was a greater total N source because of the convergence of lateral flow and near-stream riparian N sources in surface soils, which elevated stream DON and to a lesser extent NH4+. In contrast, preferential flow in the wetland catchment tended to dilute DIN in saturated peatland soils and in the stream, whereas DON varied little over time. These findings highlight the importance of hydrologic processes that store meltwater N in catchment soils but at the same time deliver DON from riparian sources to the stream. Further, model results suggest that biotic uptake and/or sorption effectively retain much of the meltwater DIN from the snowpack. Collectively, hydrologic storage and biogeochemical processes act to retain N that is likely important for boreal ecosystem production later in the

  20. In situ measurements of microbially-catalyzed nitrification and nitrate reduction rates in an ephemeral drainage channel receiving water from coalbed natural gas discharge, Powder River Basin, Wyoming, USA

    USGS Publications Warehouse

    Harris, S.H.; Smith, R.L.

    2009-01-01

    Nitrification and nitrate reduction were examined in an ephemeral drainage channel receiving discharge from coalbed natural gas (CBNG) production wells in the Powder River Basin, Wyoming. CBNG co-produced water typically contains dissolved inorganic nitrogen (DIN), primarily as ammonium. In this study, a substantial portion of discharged ammonium was oxidized within 50??m of downstream transport, but speciation was markedly influenced by diel fluctuations in dissolved oxygen (> 300????M). After 300??m of transport, 60% of the initial DIN load had been removed. The effect of benthic nitrogen-cycling processes on stream water chemistry was assessed at 2 locations within the stream channel using acrylic chambers to conduct short-term (2-6??h), in-stream incubations. The highest ambient DIN removal rates (2103????mol N m- 2 h- 1) were found at a location where ammonium concentrations > 350????M. This occurred during light incubations when oxygen concentrations were highest. Nitrification was occurring at the site, however, net accumulation of nitrate and nitrite accounted for < 12% of the ammonium consumed, indicating that other ammonium-consuming processes were also occurring. In dark incubations, nitrite and nitrate consumption were dominant processes, while ammonium was produced rather than consumed. At a downstream location nitrification was not a factor and changes in DIN removal rates were controlled by nitrate reduction, diel fluctuations in oxygen concentration, and availability of electron donor. This study indicates that short-term adaptation of stream channel processes can be effective for removing CBNG DIN loads given sufficient travel distances, but the long-term potential for nitrogen remobilization and nitrogen saturation remain to be determined.

  1. Improved analysis of dissolved organic nitrogen in water via electrodialysis pretreatment.

    PubMed

    Zhu, Anbang; Chen, Baiyang; Zhang, Liang; Westerhoff, Paul

    2015-02-17

    This study evaluated electrodialysis (ED) for direct, accurate, and precise dissolved organic nitrogen (DON) analysis in water. Unlike conventional methods that calculate DON as the difference between total dissolved nitrogen (TDN) and dissolved inorganic nitrogen (DIN), we designed a compact ED reactor as a pretreatment tool that completely separates DIN from DON in water and then measures DON by equating DON to TDN. The experiments confirmed that the ED pretreatment process can achieve 99% removal of all three major DIN species (i.e., ammonia, nitrite, and nitrate) and an average recovery rate of 88% for an array of model DON compounds of varying characteristics (e.g., urea, amino acids, tripeptide, protein, and humic substances). Variations in nitrogen removal and recovery might be explained by a combined effect of molecular weight, acid dissociation ability (pK(a)), aromaticity, and ED reactor configurations. For model solutions with DIN/DON ratios varying from 1 to 10 mg-N/mg-N, the relative standard deviations in DON concentrations were considerably lower with ED pretreatment (<10%) than without pretreatment (47%). A survey of seven field samples, including lake water, tap water, and treated wastewater, also demonstrated the benefits of using ED pretreatment as compared with a conventional DON analysis method. Overall, this study provides evidence and mechanistic insight for a new DON detection method that uses ED pretreatment. The ED unit is robust for separating DIN and DON, and thus it may facilitate more frequent detection of DON and ultimately enhances understanding of DON issues in the environmental studies. PMID:25621718

  2. A Simple Heuristic Model of Nutrient Cycling in an Estuary

    NASA Astrophysics Data System (ADS)

    Kimmerer, W. J.; Smith, S. V.; Hollibaugh, J. T.

    1993-08-01

    Three decades of discussion and study have not resolved the apparent discrepancy between N-limitation of primary production and the ability of marine ecosystems to fix N. We use a simple model as a heuristic tool to examine controls on nutrient cycling in a shallow estuary, with Tomales Bay, California as the prototype. The model is a steady-state, one-box model with inputs and losses of nutrients and organic matter, and terms representing N-fixation and denitrification. The physical description of the system is deliberately kept simple to permit a focus on the key biogeochemical reactions. Growth of autotrophs in the model can be limited either by dissolved inorganic nitrogen (DIN) or dissolved inorganic phosphorus (DIP). Nitrogen-fixation is controlled by the availability of DIP or limited by excess amounts of DIN. Model results demonstrate that, for a system with a long residence time, autotroph biomass and total organic matter are controlled primarily by the rate of delivery of P to the system, either as DIP or in organic matter. Increasing the delivery rate of DIN raises autotroph biomass slightly but has little effect on total organic matter. This is because the rates of input of P as DIP or organic matter control the N-fixation rate, and denitrification limits the build-up of DIN in the system. Thus, denitrification and N-fixation act as opposing negative feedbacks, insuring that the supply of N remains roughly commensurate with that of P. When exchange with the ocean is increased, reducing residence time, the relative importance of DIN input increases relative to that of DIP.

  3. Constraining nitrogen inputs to urban streams from leaking sewer infrastructure using inverse modeling: Implications for urban water quality

    NASA Astrophysics Data System (ADS)

    Sikora, M. T.; Elliott, E. M.; Bain, D. J.

    2011-12-01

    Excess fixed nitrogen contributes to stream degradation in densely populated regions, compounding problems of surface water contamination in urban landscapes. In particular, leaking sewer infrastructure is an acknowledged source of non-point source (NPS) nitrogen pollution to ground- and surface water in urban areas; however quantification of such contributions is exceedingly limited. This lack of knowledge inhibits efforts to understand urban nitrogen retention and export, despite the potential for this source to impact downstream water quality. Nine Mile Run (NMR), a restored urban stream in Pittsburgh, Pennsylvania (USA), drains a 1600 hectare urban watershed characterized by a high degree of impervious surface cover (38%). For years known locally as "stink creek," NMR remains significantly impacted by combined sewer overflows, leaky sewers, and degraded water quality. In order to assess sources of impairment, water samples were collected from four locations bi-weekly over two years, intensive sampling was conducted during one summer storm and DIN concentrations in water samples were analyzed (where DIN = nitrate + nitrite + ammonium). Using DIN concentrations, discharge records, published estimates of urban watershed nitrogen retention, and known inputs of atmospherically deposited nitrogen, a watershed nitrogen budget was constructed for NMR and subsequently inverted to constrain potential sewage inputs. Retention estimates ranging from 65 to 85% were applied and resulting calculations indicate that DIN contributions from sewage ranged from 5.5 to 25 kg ha-1yr-1. This research documents the potential contribution of sewage to DIN loads in urban streams and highlights the challenges of reducing nutrient pollution to receiving waters in cities with aging, degraded sewer lines.

  4. Effects of environmental variables on between-year variation of Ulva growth and biomass in a eutrophic brackish lake

    NASA Astrophysics Data System (ADS)

    Malta, Erik-jan; Verschuure, Jacobus M.

    1997-12-01

    Biomass development, growth rate, tissue composition and habitat characteristics of macroalgal blooms were monitored in the eutrophic Veerse Meer (the Netherlands) in 1992 and in 1994 to determine seasonal and between-year variabilities and their relation with environmental factors. In both years, the blooms were dominated by Ulva species (more than 95% of total macroalgal biomass). In 1992, the maximum biomass was 602 g DW m -2, in 1994 the maximum biomass was only 282 g DW m -2. Growth rates (μ), measured in cages, were high at the beginning of May 1992, but quickly dropped to values between 0.05 and 0.10 day -1. In 1994, high growth rates were observed for 1 week in early May only. Water nitrogen concentrations (DIN) and tissue nitrogen levels in Ulva spp. were higher in 1994 than in 1992. No overall difference was found in irradiance between 1992 and 1994 at the beginning of the growing season, but irradiance levels were much higher in 1992. The results of a stepwise multiple regression analysis indicate that in 1992 the part of variation in growth rate that could be explained by regression was due to water DIN. In 1994, water phosphorus concentration and light were the variables explaining this part of the variation in growth rate. It is concluded that macroalgal biomass development in the Veerse Meer shows high variability, both within one season and between years. Although positive correlations were shown between tissue nitrogen levels and DIN, differences in DIN could not explain between-year variability. In a eutrophic lagoon, incident irradiance levels are probably more important in regulating maximum macroalgal yield than DIN.

  5. Seasonal Patterns of Nitrogen and Phosphorus Limitation in Four German Lakes and the Predictability of Limitation Status from Ambient Nutrient Concentrations

    PubMed Central

    Kolzau, Sebastian; Wiedner, Claudia; Rücker, Jacqueline; Köhler, Jan; Köhler, Antje; Dolman, Andrew M.

    2014-01-01

    To identify the seasonal pattern of nitrogen (N) and phosphorus (P) limitation of phytoplankton in four different lakes, biweekly experiments were conducted from the end of March to September 2011. Lake water samples were enriched with N, P or both nutrients and incubated under two different light intensities. Chlorophyll a fluorescence (Chla) was measured and a model selection procedure was used to assign bioassay outcomes to different limitation categories. N and P were both limiting at some point. For the shallow lakes there was a trend from P limitation in spring to N or light limitation later in the year, while the deep lake remained predominantly P limited. To determine the ability of in-lake N:P ratios to predict the relative strength of N vs. P limitation, three separate regression models were fit with the log-transformed ratio of Chla of the P and N treatments (Response ratio = RR) as the response variable and those of ambient total phosphorus:total nitrogen (TN:TP), dissolved inorganic nitrogen:soluble reactive phosphorus (DIN:SRP), TN:SRP and DIN:TP mass ratios as predictors. All four N:P ratios had significant positive relationships with RR, such that high N:P ratios were associated with P limitation and low N:P ratios with N limitation. The TN:TP and DIN:TP ratios performed better than the DIN:SRP and TN:SRP in terms of misclassification rate and the DIN:TP ratio had the highest R2 value. Nitrogen limitation was predictable, frequent and persistent, suggesting that nitrogen reduction could play a role in water quality management. However, there is still uncertainty about the efficacy of N restriction to control populations of N2 fixing cyanobacteria. PMID:24755935

  6. Estimating the terrestrial N processes in subtropical mountainous forestry catchment through INCA-N: A case study in FuShan catchment, Taiwan

    NASA Astrophysics Data System (ADS)

    Lu, Meng-Chang; Huang, -Chuan, Jr.; Chang, Chung-Te; Shih, Yu-Ting; Lin, Teng-Chiu

    2016-04-01

    The riverine DIN is a crucial indicator for eutrophication in river network. The riverine DIN export in Taiwan is featured by the extremely high yield, ~3800 kg-N km-2yr-1, nearly 20-fold than the global average, showing the interesting terrestrial N process yet rarely documented. In this study we collected the DIN samples in rainwater, soil water, and stream water in a mountainous forest watershed, FuShan experimental forest watershed 1 (WS1) which is a natural broadleaf forest without human activities. Based on the intensive observations, we applied the INCA-N to simulate the riverine DIN response and thus estimate the terrestrial N processes in a global synthesis. The result showed that both discharge and DIN yield were simulated well with the average Nash-Sutcliffe efficiency coefficient of 0.83 and 0.76 , respectively. Among all N processes, N uptake, mineralization, nitrification, denitrfication, and immobilization are significantly positive correlated with soil moisture (R2>0.99), which indicates that soil moisture greatly influences N cycle processes. The average rate of mineralization and nitrification in wet years are consistent with documented values, whereas the rates in dry years are lower than the observations. Despite the high nitrification rate, the secondary forest may uptake abundant N indicating the plant uptake, which responds for removing considerable nitrate, is a controlling factor in forest ecosystem. Our simulated denitrification rate falls between the documented rates of temperate forest and agricultural area, and that may be affected by the high N-deposition in Taiwan. Simulated in-stream denitrification rate is less than 10% of the rate in soil, and is a little lower than that in temperate forest. This preliminary simulation provides an insightful guide to establish the monitoring programme and improve the understanding of N cycle in subtropical.

  7. FOREWORD: VI Workshop in Electron Volt Neutron Spectroscopy: Frontiers and Horizons

    NASA Astrophysics Data System (ADS)

    Seel, A. G.; Senesi, R.; Fernandez-Alonso, F.

    2014-12-01

    January 2014 saw the congregation in Abingdon (UK) of scientists from across the world, to discuss the current state and future of spectroscopy using epithermal neutrons. This meeting was the sixth in a series of workshops held in collaboration between the Science and Technology Facilities Council (UK) and the Consiglio Nazionale delle Ricerche (Italy), aimed at bringing together researchers with an interest in the use of electron-volt neutrons in spectroscopic studies [1]. This technique is termed Deep Inelastic Neutron Scattering (DINS), and also Neutron Compton Scattering (NCS) in reference to the analogy with Compton scattering of X-rays from electrons. In particular, this meeting centred jointly around experimentalists and theoreticians, formulating animated discussions as to the current overview of DINS and future horizons facing the field. The use of electron Volt neutrons for spectroscopic measurements dates back to the advent of proton-driven spallation neutron sources in the 1970s and 1980s. Following an initial scientific meeting in Los Alamos (USA) [2], the first two meetings in this series were held in Abingdon (UK) in 1995 and 1998, with subsequent meetings held in Santa Fe (USA) in 2005 [3], Oak Ridge National Laboratory (USA) in 2006 [4], and Rome, Italy; in 2010 [5]. The flagship instrument serving a continual user-programme for DINS measurements, and the main focus of this meeting, has been the VESUVIO spectrometer at ISIS [6, 7]. Subsequent instruments like SEQUOIA in the USA [8, 9] and a newer spectrometer in the Bariloche LINAC in Argentina [10, 11] have also been commissioned and began serving a growing user community. The availability of DINS measurements has extended the range of possible spectroscopic techniques that utilise neutrons into the kinematic region of high energy and momentum transfers, shown schematically in Figure 1. Spectroscopic instrument suites such as that of ISIS are thus able to probe processes on timescales across nine

  8. Mapping Slumgullion Landslide in Colorado, USA Using Airborne Repeat-Pass InSAR

    NASA Astrophysics Data System (ADS)

    Lee, H.; Shrestha, R. L.; Carter, W. E.; Glennie, C. L.; Wang, G.; Lu, Z.; Fernandez-Diaz, J. C.; Cao, N.; Zaugg, E.

    2015-12-01

    Interferometric Synthetic Aperture Radar (InSAR) uses two or more SAR images over the same area to determine landscape topography or ground deformation. An interferogram, generated by the phase components of two coherent SAR images, depicts range changes between the radar and the ground resolution elements, and can be used to derive both landscape topography and subtle changes in surface elevation. However, spaceborne repeat-pass interferometry has two main drawbacks: effects due to differences in atmospheric temperature, pressure, and water vapour at two observation times, and loss of coherence due to long spatial and temporal baselines between observations. Airborne repeat-pass interferometry does not suffer from these drawbacks. The atmospheric effect in case of airborne DInSAR becomes negligible due to smaller swath coverage, and the coherence can be maintained by using smaller spatial and temporal baselines. However, the main technical limitation concerning airborne DInSAR is the need of precise motion compensation with an accurate navigation system to correct for the significant phase errors due to typical flight instability from air turbulence. Here, we present results from a pilot study conducted on July 2015 using both X-band and L-band SlimSAR airborne system over the Slumgullion landslide in Colorado in order to (1) acquire the differential interferograms from the airborne platform, (2) understand their source of errors, and (3) pave a way to improve the precision of the derived surface deformation. The landslide movement estimated from airborne DInSAR is also compared with coincident GPS, terrestrial laser scanning (TLS), airborne LiDAR, and spaceborne DInSAR measurements using COSMO-SkyMed images. The airborne DInSAR system has a potential to provide time-transient variability in land surface topography with high-precision and high-resolution, and provide researchers with greater flexibility in selecting the temporal and spatial baselines of the data

  9. Summer nutrients structure and phytoplankton growth under the influence of freshwater-saline water mixing in the Changjiang River Estuary

    NASA Astrophysics Data System (ADS)

    Liu, Ying; Wang, Kui; Chen, Jianfang

    2015-04-01

    The spatial distribution of NO3-, PO43-, SiO32- in summer were studied in the Changjiang (Yangtze River) Estuary and the adjacent East China Sea(ECS), the nutrients distribution was mainly controlled by the Changjiang dilution water and offshore seawater mixing. NO3-, PO43-, SiO32- were high west and low east, presenting two tongues shape nearshore. Combined with the nutrient structure difference along the estuary gradient, the potential relative nutrient limitation of surface water at each station was distinguished. Coastal water was featured with excess nitrogen, in summer DIN/P ratio was up to 160 in the frequent algae blooms area, while Si/N reached as low as 0.5, which could be caused by luxury consumption of P and Si by diatom bloom. For better understanding the process of nutrients structure variation and influence on phytoplankton growth under the Changjiang dilution water and seawater mixing, we also conducted field incubation simulating different fresh-saline water mixing scale, by 100%, 75%, 50%, 25% and 0% for about 3 days. The results were as follows: (1) The lower the percentage of freshwater, the lower the growth rate and pH increase rate of phytoplankton during the exponential growth period; (2) Macronutrients were apparently consumed. PO43- in the 100%, 75% and 50% dilution treatments were depleted within 48 h, suggesting that PO43- limit phytoplankton growth below salinity of 26. (3) For the 100% treatment the DIN/P ratio doubled as PO43- was consumed rapidly, while DIN decreased slowly. The DIN/Si ratio decreased to about 0.7 times the original level during the first 48 h, reflecting the lower initial DIN/Si value compared to the diatom uptake ratio (dDIN/dSi) during the incubation period. The incubation presented the phytoplankton growth extent and rate difference during fresh-saline water mixing, which makes nutrients gradient, and this mixing process may cause local blooms to change the nutrient structure, then might result in phytoplankton

  10. Export of dissolved inorganic nutrients to the northern Indian Ocean from the Indian monsoonal rivers during discharge period

    NASA Astrophysics Data System (ADS)

    Krishna, M. S.; Prasad, M. H. K.; Rao, D. B.; Viswanadham, R.; Sarma, V. V. S. S.; Reddy, N. P. C.

    2016-01-01

    Coastal regions are highly productive due to the nutrients largely supplied by rivers. To examine the contribution of dissolved inorganic nutrients (DIN) by Indian rivers to coastal waters, data were collected near the freshwater heads of 27 monsoonal rivers of peninsular India during three weeks in late July to mid-August, the middle of the principal runoff period of the southwest monsoon of 2011. Twelve researchers in four groups, equipped with car and portable laboratory equipment, sampled mid-stream of each estuary using mechanized boat, and filtered and partly analyzed the water in the evening. The estimated exports were 0.22 ± 0.05, 0.11 ± 0.03, and 1.03 ± 0.26 Tg yr-1 for dissolved inorganic nitrogen, phosphorus and silicate, respectively. Higher amounts of DIN reach the Bay of Bengal than the Arabian Sea due to the higher volume (∼76%) of discharge to the former. In contrast, the export of dissolved inorganic nitrogen is almost same to the Bay of Bengal (0.12 ± 0.03 Tg yr-1) and Arabian Sea (0.10 ± 0.02 Tg yr-1) principally due to the polluted Narmada and Tapti rivers in the northwest. Including input from the glacial rivers, Ganges, Brahmaputra and Indus, it is estimated that the northern Indian Ocean receives ∼1.84 ± 0.46, 0.28 ± 0.07 and 3.58 ± 0.89 Tg yr-1 of nitrate, phosphate and silicate, respectively, which are significantly lower than the earlier estimates of DIN export from the Indian rivers based on DIN measured in the mid or upstream rivers. Such low fluxes in this study were attributed to efficient retention/elimination of DIN (∼91%) before reaching the coastal ocean. Hence, this study suggests that the importance of sampling locations for estimating nutrient fluxes to the coastal ocean. Riverine DIN export of 1.84 ± 0.46 Tg yr-1 would support 12.2 ± 3.1 Tg C yr-1 of new production in coastal waters of the northern Indian Ocean that results in a removal of 12.2 ± 3.1 Tg atmospheric CO2 yr-1.

  11. Two-dimensional Co-Seismic Surface Displacements Field of the Chi-Chi Earthquake Inferred from SAR Image Matching

    PubMed Central

    Hu, Jun; Li, Zhi-Wei; Ding, Xiao-Li; Zhu, Jian-Jun

    2008-01-01

    The Mw=7.6 Chi-Chi earthquake in Taiwan occurred in 1999 over the Chelungpu fault and caused a great surface rupture and severe damage. Differential Synthetic Aperture Radar Interferometry (DInSAR) has been applied previously to study the co-seismic ground displacements. There have however been significant limitations in the studies. First, only one-dimensional displacements along the Line-of-Sight (LOS) direction have been measured. The large horizontal displacements along the Chelungpu fault are largely missing from the measurements as the fault is nearly perpendicular to the LOS direction. Second, due to severe signal decorrelation on the hangling wall of the fault, the displacements in that area are un-measurable by differential InSAR method. We estimate the co-seismic displacements in both the azimuth and range directions with the method of SAR amplitude image matching. GPS observations at the 10 GPS stations are used to correct for the orbital ramp in the amplitude matching and to create the two-dimensional (2D) co-seismic surface displacements field using the descending ERS-2 SAR image pair. The results show that the co-seismic displacements range from about -2.0 m to 0.7 m in the azimuth direction (with the positive direction pointing to the flight direction), with the footwall side of the fault moving mainly southwards and the hanging wall side northwards. The displacements in the LOS direction range from about -0.5 m to 1.0 m, with the largest displacement occuring in the northeastern part of the hanging wall (the positive direction points to the satellite from ground). Comparing the results from amplitude matching with those from DInSAR, we can see that while only a very small fraction of the LOS displacement has been recovered by the DInSAR mehtod, the azimuth displacements cannot be well detected with the DInSAR measurements as they are almost perpendicular to the LOS. Therefore, the amplitude matching method is obviously more advantageous than the DIn

  12. Abiotic factors influencing biomass accumulation of green tide causing Ulva spp. on Pyropia culture rafts in the Yellow Sea, China.

    PubMed

    Keesing, John K; Liu, Dongyan; Shi, Yajun; Wang, Yujue

    2016-04-15

    Annually recurrent green-tides in the Yellow Sea have been shown to result from direct disposal into the sea of fouling Ulva from Pyropia aquaculture. The role abiotic factors play in Ulva biomass accumulation on rafts was studied to find ways to mitigate this problem. Dissolved inorganic nitrogen (DIN) was very high at all sites, but the highest Ulva biomass was associated with the lowest DIN and anthropogenic N. Under luxuriant background nutrient conditions, variability in temperature and periods of emersion, rather than pH, light and salinity determined Ulva biomass. Two dominant species of Ulva displayed differing tolerances to temperature and desiccation which helped explain why Ulva prolifera dominates floating green-tides. Rather than trying to mitigate green-tides only by reducing nutrient pollution, an earlier harvest of Pyropia in southern Jiangsu Province especially before temperatures increase greatly above 10°C during April, could reduce the biomass of U. prolifera disposed from rafts. PMID:26936121

  13. Estructura orbital en el Problema Restringido Rectilíneo Isósceles

    NASA Astrophysics Data System (ADS)

    Orellana, R. B.

    Para definir problemas en Mecánica Celeste se utilizan diferentes parámetros. El conocimiento de la dinámica del problema para valores particulares de estos parámetros nos permite entender el comportamiento en casos más generales. El Problema Restringido Rectilíneo Isósceles puede ser considerado como el caso límite del Problema de Sitnikov cuando la excentricidad tiende a uno o como el Problema Isósceles cuando la masa central tiende a cero. Se ha compactificado el espacio de fases y analizado la dinámica en el límite. Esto ha permitido separar el espacio de fases en diferentes regiones dependiendo de las clases de órbitas.

  14. Ammonium release from a blanket peatland into headwater stream systems.

    PubMed

    Daniels, S M; Evans, M G; Agnew, C T; Allott, T E H

    2012-04-01

    Hydrochemical sampling of South Pennine (UK) headwater streams draining eroded upland peatlands demonstrates these systems are nitrogen saturated, with significant leaching of dissolved inorganic nitrogen (DIN), particularly ammonium, during both stormflow and baseflow conditions. DIN leaching at sub-catchment scale is controlled by geomorphological context; in catchments with low gully densities ammonium leaching dominates whereas highly gullied catchments leach ammonium and nitrate since lower water tables and increased aeration encourages nitrification. Stormflow flux calculations indicate that: approximately equivalent amounts of nitrate are deposited and exported; ammonium export significantly exceeds atmospheric inputs. This suggests two ammonium sources: high atmospheric loadings; and mineralisation of organic nitrogen stored in peat. Downstream trends indicate rapid transformation of leached ammonium into nitrate. It is important that low-order headwater streams are adequately considered when assessing impacts of atmospheric loads on the hydrochemistry of stream networks, especially with respect to erosion, climate change and reduced precipitation. PMID:22285801

  15. Increased groundwater to stream discharge from permafrost thawing in the Yukon River basin: Potential impacts on lateral export of carbon and nitrogen

    USGS Publications Warehouse

    Walvoord, M.A.; Striegl, R.G.

    2007-01-01

    Arctic and subarctic watersheds are undergoing climate warming, permafrost thawing, and thermokarst formation resulting in quantitative shifts in surface water - groundwater interaction at the basin scale. Groundwater currently comprises almost one fourth of Yukon River water discharged to the Bering Sea and contributes 5-10% of the dissolved organic carbon (DOC) and nitrogen (DON) and 35-45% of the dissolved inorganic carbon (DIC) and nitrogen (DIN) loads. Long-term strearnflow records (>30 yrs) of the Yukon River basin indicate a general upward trend in groundwater contribution to streamflow of 0.7-0.9%/yr and no pervasive change in annual flow. We propose that the increases in groundwater contributions were caused predominately by climate warming and permafrost thawing that enhances infiltration and supports deeper flowpaths. The increased groundwater fraction may result in decreased DOC and DON and increased DIC and DIN export when annual flow remains unchanged.

  16. Department of Defense picture archiving and communication system acceptance testing: results and identification of problem components.

    PubMed

    Allison, Scott A; Sweet, Clifford F; Beall, Douglas P; Lewis, Thomas E; Monroe, Thomas

    2005-09-01

    The PACS implementation process is complicated requiring a tremendous amount of time, resources, and planning. The Department of Defense (DOD) has significant experience in developing and refining PACS acceptance testing (AT) protocols that assure contract compliance, clinical safety, and functionality. The DOD's AT experience under the initial Medical Diagnostic Imaging Support System contract led to the current Digital Imaging Network-Picture Archiving and Communications Systems (DIN-PACS) contract AT protocol. To identify the most common system and component deficiencies under the current DIN-PACS AT protocol, 14 tri-service sites were evaluated during 1998-2000. Sixteen system deficiency citations with 154 separate types of limitations were noted with problems involving the workstation, interfaces, and the Radiology Information System comprising more than 50% of the citations. Larger PACS deployments were associated with a higher number of deficiencies. The most commonly cited systems deficiencies were among the most expensive components of the PACS. PMID:15924273

  17. Monitoring of Land Surface Displacements within the Areas with Intensnsive Oil Production Using Satellite Remote Sensing Data

    NASA Astrophysics Data System (ADS)

    Nugmanov, Ilmir; Olga, Chernova

    2015-05-01

    The purpose of the study is to assess the informative value of differential synthetic aperture radar interferometry (DInSAR) as applied to estimation of modern crustal movements within the oil-producing regions of the Republic of Tatarstan (RT). For the first time in the RT, the earth’s surface displacement maps were created using ERS and ALOS PALSAR data for different time periods in order to show possibility of using radar data to monitor urban areas. Interferometry results were then compared with geodetic leveling data. It is obvious that DInSAR resolution is incomparably greater than resolution of traditional methods used for monitoring of the earth's surface displacements. In the authors’ opinion, the technology of using radar data to monitor the earth’s surface displacements can become a principal approach to forecasting and prevention of industrial and natural disasters any time soon.

  18. Do-It-Now building maintenance reengineering project

    SciTech Connect

    Apoddaca, L.; Ayotte, A.; Coffman, J.; Dukes, L.; Jojola, S.; Kadlec, J.; Letourneau, S.; Matthews, R.; Paul, G.

    1996-09-01

    The Do-It-Now (DIN) building maintenance system is proposed to reduce the cost of routine building maintenance and repairs and to improve customer satisfaction with maintenance services. DIN uses a team approach to periodically inspect buildings and provide maintenance services on the spot. It emphasizes communications between the customers and the craftspeople performing the work. The system was designed using a reengineering approach that characterized the existing maintenance work control system, analyzed comparable systems in other DOE laboratories, envisioned an ideal system, and proposed a workable, testable system for initial implementation. At each stage, input was solicited from customer representatives and Facilities management to ensure meeting customer requirements with an implementable system.

  19. Nitrogen stable isotope ratio in the manila clam, Ruditapes philippinarum, reflects eutrophication levels in tidal flats.

    PubMed

    Watanabe, Satoshi; Kodama, Masashi; Fukuda, Masaaki

    2009-10-01

    Understanding the effects of anthropogenic eutrophication on coastal fisheries may help in the enhancement of fishery production by effective utilization of sewage effluents, as well as in the consequent reduction of eutrophication. In this study, it was revealed that the nitrogen stable isotope ratio (delta(15)N) in the soft tissues of the manila clam, Ruditapes philippinarum, can be used as an indicator of anthropogenic eutrophication levels in tidal flat environments by investigation of delta(15)N in dissolved inorganic nitrogen (DIN), particulate organic matter (POM), sedimentary organic matter (SOM) and soft tissues of the clam in five tidal flats in Japan with different levels of DIN concentration. In addition, it was found that the acid insoluble fraction of the shell organic matrix, conchiolin, can be used as a proxy for the soft tissues in delta(15)N analyses. This will contribute in easier storage handling and the expansion of chances for sample acquisition. PMID:19647270

  20. Environmental Factors and Seasonal Occurrence of the Dinoflagellate Alexandrium minutum, a PSP Potential Producer, in a Mediterranean Lagoon

    NASA Astrophysics Data System (ADS)

    Giacobbe, M. G.; Oliva, F. D.; Maimone, G.

    1996-05-01

    Seasonal fluctuations of hydrobiological parameters were studied, throughout 1992, in a shallow Mediterranean area (Ganzirri lagoon), affected by the dinoflagellate Alexandrium minutum, a potential producer of saxitoxins. Concentrations of dissolved inorganic nitrogen (DIN) and N:P ratios were high in autumn and winter, when the waters were well mixed and free from A. minutumcells. The spring appearance of this species coincided with enhanced rainfall and freshwater runoff and with stabilization of the water column, whereas cell dispersion was observed in summer. Although A. minutumnever dominated the algal community, cell densities were maximal in May, when values of salinity did not exceed 30; during this month, there were reduced amounts of DIN, N:P atomic ratios close to 16:1 and a minor competitive pressure by other phytoplankters. Subsequent increases in values of the above parameters—in particular, of the ammonium nitrogen—were associated with a decline in cell numbers, and final disappearance of A. minutumcells.

  1. The monitoring of glacial migration in Greenland by multi sensor data fusion

    NASA Astrophysics Data System (ADS)

    Park, H.; Kim, J.; yun, H.; Choi, Y.

    2013-12-01

    The retreatment of ice sheet in Greenland by global warming is now firmly confirmed by variety of observations. However, the arguments about the melting speed of ice sheet is still undergoing owing to the absence of the quantitative measurement method to continuously trace the direction and the speed of icecap change. Thus, we tested a scheme to spatially and temporally monitor the migration of ice sheet with high accuracy employing multi sensor information and their data fusion. The target areas were established in Jakoshavm and Mittivakkat glaciers where the shrinking of glaciers has been obvious for the last one century. At first, in both well-known Greenland glaciers, Differential Interferometric SAR (DInSAR) campaigns which are highly useful to monitor the glacial retreat have been undertaking employing 30 European remote sensing satellite (ERS) and 15 Advanced Land Observing Satellite (ALOS) Phased Array type L-band Synthetic Aperture Radar (PALSAR) InSAR pairs. Since base DEM quality for DInSAR analysis is poor and InSAR pixels are frequently de-correlated over ice body, two pass DInSAR doesn't produce reliable outputs. Thus the time series analyses of Permanent Scatterer and Small Baseline Subset are expected to effectively trace the translation of the glaciers and employed as the main methodology of DInSAR. Although DInSAR time series analysis effectively reconstructed temporal change of glaciers, the extracted glacial movements are projected values into line of sight direction of the sensor. Therefore it might be better to interpret them together with the other remote sensed data set for the fulfilment of useful directional measurements. In terms of such data fusion aspect, we also employ Co-registration of Optically Sensed Images and Correlation (CoSi-CORR, Leprince et al., 2007) technique, which is known as a tool for 2D change tracing, with ALOS Panchromatic Remote-sensing Instrument for Stereo Mapping (PRISM) to compensate for any line

  2. Precipitation of Laves phase in a 28%Cr-4%Ni-2%Mo-Nb superferritic stainless steel

    SciTech Connect

    Fontoura de Andrade, Thiago; Madeira Kliauga, Andrea; Plaut, Ronald Lesley; Padilha, Angelo Fernando

    2008-05-15

    The main objective of the present work was to study the precipitation of the Laves phase in the X1 CrNiMoNb 28 4 2 (Werkstoff-Nr. DIN 1.4575) superferritic stainless steel employing several complementary techniques of microstructural analysis. The phase that precipitated in largest quantity in the DIN 1.4575 steel was the sigma ({sigma}) phase. However, along grain boundaries, after aging at 850 deg. C, a Laves phase of the MgZn{sub 2} type, with a hexagonal C14 crystal structure and chemical composition (Fe,Cr,Ni){sub 2}(Nb,Mo,Si), was also identified. Growth of the Laves phase is curtailed by exhaustion of niobium of the matrix and by the presence of the sigma phase, which also precipitates in the vicinity of the grain boundaries, however in larger amounts. No chi ({chi}) or austenite phases were detected in the temperature range studied.

  3. Nutrient limitation of phytoplankton growth in Georgia nearshore waters

    SciTech Connect

    Bishop, S.S.; Emmanuele, K.A.; Yoder, J.A.

    1984-12-01

    Nutrient enrichment experiments were conducted to investigate the utilization of dissolved organic (DON) and inorganic nitrogen (DIN) by marine phytoplankton in Georgia coastal waters. Natural populations of marine phytoplankton, enriched with different concentrations of ammonium chloride and other plant nutrients, were grown under controlled temperature and irradiance conditions until the populations reached ''stationary phase.'' Results showed that (1) phytoplankton are limited by DIN up to ca. 20 ..mu..M, when another nutrient (phosphate or silicate) becomes limiting, (2) very little naturally-occuring DON is directly utilized for growth, (3) very little DON is indirectly made available for growth over time periods of days to ca. 1 week, and (4) trace metals and vitamins do not significantly limit phytoplankton growth.

  4. Correlation of in vivo relative bioavailability to in vitro bioaccessibility for arsenic in household dust from China and its implication for human exposure assessment.

    PubMed

    Li, Hong-Bo; Li, Jie; Juhasz, Albert L; Ma, Lena Q

    2014-12-01

    Incidental ingestion of household dust is an important arsenic (As) exposure pathway for children. However, compared to soils, assessment of As relative bioavailability (RBA) in household dust is limited. In this study, As-RBA in 12 household dust samples (7–38 mg kg(–1)) was measured using an in vivo mouse model and compared to As bioaccessibility determined using 4 assays [Solubility Bioaccessibility Research Consortium method (SBRC), in vitro gastrointestinal method (IVG), Deutsches Institut für Normunge.V. method (DIN), and physiologically based extraction test (PBET)]. Arsenic RBA ranged from 21.8 ± 1.6 to 85.6 ± 7.2% with samples containing low Fe and high total organic carbon content having higher As-RBA. Strong in vivo–in vitro correlations (IVIVC) were found between As-RBA and As bioaccessibility for SBRC and DIN (r2 = 0.63–0.85), but weaker ones were obtained for IVG and PBET (r2 = 0.29–0.55). The developed IVIVC for SBRC and DIN were used to calculate As-RBA based on As bioaccessibility for an additional 12 household dust samples. Although As bioaccessibility differed significantly (up to 7.7-fold) based on in vitro methods, predicted As-RBA was less variable (up to 3.0-fold) when calculated using As bioaccessibility data and the corresponding IVIVC. Our data suggested that both SBRC and DIN had potential to assess As bioavailability in household dust samples; however, additional research is needed. PMID:25365687

  5. RNA polymerase mutations that facilitate replication progression in the rep uvrD recF mutant lacking two accessory replicative helicases.

    PubMed

    Baharoglu, Zeynep; Lestini, Roxane; Duigou, Stéphane; Michel, Bénédicte

    2010-07-01

    We observed that cells lacking Rep and UvrD, two replication accessory helicases, and the recombination protein RecF are cryo-sensitive on rich medium. We isolated five mutations that suppress this Luria-Bertani (LB)-cryo-sensitivity and show that they map in the genes encoding the RNA polymerase subunits RpoB and RpoC. These rpoB (D444G, H447R and N518D) and rpoC mutants (H113R and P451L) were characterized. rpoB(H447R) and rpoB(D444G) prevent activation of the Prrn core promoter in rich medium, but only rpoB(H447R) also suppresses the auxotrophy of a relA spoT mutant (stringent-like phenotype). rpoC(H113R) suppresses the thermo-sensitivity of a greA greB mutant, suggesting that it destabilizes stalled elongation complexes. All mutations but rpoC(P451L) prevent R-loop formation. We propose that these rpo mutations allow replication in the absence of Rep and UvrD by destabilizing RNA Pol upon replication-transcription collisions. In a RecF(+) context, they improve growth of rep uvrD cells only if DinG is present, supporting the hypothesis that Rep, UvrD and DinG facilitate progression of the replication fork across transcribed sequences. They rescue rep uvrD dinG recF cells, indicating that in a recF mutant replication forks arrested by unstable transcription complexes can restart without any of the three known replication accessory helicases Rep, UvrD and DinG. PMID:20497334

  6. Sources, behaviors and degradation of dissolved organic matter in the East China Sea

    NASA Astrophysics Data System (ADS)

    Chen, Yan; Yang, Gui-Peng; Liu, Li; Zhang, Peng-Yan; Leng, Wei-Song

    2016-03-01

    Concentrations of dissolved organic carbon (DOC), dissolved inorganic nitrogen (DIN), dissolved organic nitrogen (DON) and its major compound classes-total hydrolysable amino acids (THAA) were measured at 4 cross-shelf transects of the East China Sea in July 2011. Surface concentrations of DOC, DIN, DON and THAA at the nearshore stations were mostly in excess of those found at the offshore sites, indicating either substantial autochthonous production or allochthonous inputs from the Changjiang River. The vertical distributions of DOC, DON and THAA showed similar trends with higher values in the surface layer, whereas the elevated concentrations of DIN were observed in the bottom layer. Major constituents of THAA presented in the study area were glycine, serine, alanine, glutamic acid, aspartic acid and valine. The mole percentages of neutral amino acids increased from surface water to bottom water, whereas acidic and hydroxy amino acids decreased with the water depth. Concentrations of DOC and THAA were negatively correlated to the ΔDIN values (the difference between the real concentration and theoretical concentration), respectively, indicating the coupling relation between dissolved organic matter (DOM) remineralization and nutrient regeneration in the water column. The C/N ratios in the water column exhibited different characteristics with elevated values appearing in the surface and bottom layers. Box and whisker plots showed that both degradation index (DI) values and THAA yields displayed a decreasing trend from the surface layer to the bottom layer, implying increasing degradation with the water depth. Our data revealed that glycine and alanine increased in relative abundance with decreasing DI, while tyrosine, valine, phenylalanine and isoleucine increased with increasing DI.

  7. Virtual management of radiology examinations in the virtual radiology environment using common object request broker architecture services.

    PubMed

    Martinez, R; Rozenblit, J; Cook, J F; Chacko, A K; Timboe, H L

    1999-05-01

    In the Department of Defense (DoD), US Army Medical Command is now embarking on an extremely exciting new project--creating a virtual radiology environment (VRE) for the management of radiology examinations. The business of radiology in the military is therefore being reengineered on several fronts by the VRE Project. In the VRE Project, a set of intelligent agent algorithms determine where examinations are to routed for reading bases on a knowledge base of the entire VRE. The set of algorithms, called the Meta-Manager, is hierarchical and uses object-based communications between medical treatment facilities (MTFs) and medical centers that have digital imaging network picture archiving and communications systems (DIN-PACS) networks. The communications is based on use of common object request broker architecture (CORBA) objects and services to send patient demographics and examination images from DIN-PACS networks in the MTFs to the DIN-PACS networks at the medical centers for diagnosis. The Meta-Manager is also responsible for updating the diagnosis at the originating MTF. CORBA services are used to perform secure message communications between DIN-PACS nodes in the VRE network. The Meta-Manager has a fail-safe architecture that allows the master Meta-Manager function to float to regional Meta-Manager sites in case of server failure. A prototype of the CORBA-based Meta-Manager is being developed by the University of Arizona's Computer Engineering Research Laboratory using the unified modeling language (UML) as a design tool. The prototype will implement the main functions described in the Meta-Manager design specification. The results of this project are expected to reengineer the process of radiology in the military and have extensions to commercial radiology environments. PMID:10342205

  8. A Connector Between Active Laboratory Probes and Computer Interfaces of Different Brands

    NASA Astrophysics Data System (ADS)

    Hui, Kaleonui J.; Coney, John P.; Binder, P.-M.

    2016-04-01

    In PASCO's Technical Article # 763, it is stated that "PASCO [also] makes sensors with an 8-pin DIN connector. These sensors cannot be used with the Vernier interfaces, as they have different power requirements." Recently we had the need to make such connections in a 10-station freshman lab. Since the Vernier input is a 6-way BT 631A plug also used in TI and Casio equipment, our experience may be of interest to a broad audience of lab coordinators.

  9. Standardization of open-path FTIR measurements in Germany

    NASA Astrophysics Data System (ADS)

    Weber, Konradin; Eickel, Karl-Heinz

    1997-05-01

    The 'Commission of Air Pollution Prevention in VDI and DIN', which is officially responsible for the setting of written standards in the field of air pollution in Germany, has established a working group for standardization of open-path FTIR measurements in Germany. This group has presently worked out has worked out a draft for a VDI-guideline, in which it is laid down, how to perform measurements with the open-path FTIR to get reliable results.

  10. Testing of new banknotes for machines that process currency

    NASA Astrophysics Data System (ADS)

    Foster, Eugenie E.

    2000-04-01

    Banknotes are now frequently use din machines. The Federal Reserve Board and the US Department of the Treasury have identified a need to produce notes that are reliably accepted in a variety of machine applications. This paper describes the steps that led to identifying requirements of manufacturers of machines that process banknotes for test notes, and the program developed for the Bureau of Engraving and Printing to address those requirements.

  11. Observación solar desde el espacio, resultados recientes

    NASA Astrophysics Data System (ADS)

    Machado, M. E.

    Presentaremos un resumen de los resultados más recientes sobre la física del sol, obtenidos por medio del análisis de datos de satélites artificiales como el Yohkoh, SOHO y COMPTON/GRO. En particular, nos referiremos a la acción y dinámica de los campos magnéticos en la generación de fenómenos activos y el calentamiento coronal.

  12. Testing the AUDI2000 colour-difference formula for solid colours using some visual datasets with usefulness to automotive industry

    NASA Astrophysics Data System (ADS)

    Martínez-García, Juan; Melgosa, Manuel; Gómez-Robledo, Luis; Li, Changjun; Huang, Min; Liu, Haoxue; Cui, Guihua; Luo, M. Ronnier; Dauser, Thomas

    2013-11-01

    Colour-difference formulas are tools employed in colour industries for objective pass/fail decisions of manufactured products. These objective decisions are based on instrumental colour measurements which must reliably predict the subjective colour-difference evaluations performed by observers' panels. In a previous paper we have tested the performance of different colour-difference formulas using the datasets employed at the development of the last CIErecommended colour-difference formula CIEDE2000, and we found that the AUDI2000 colour-difference formula for solid (homogeneous) colours performed reasonably well, despite the colour pairs in these datasets were not similar to those typically employed in the automotive industry (CIE Publication x038:2013, 465-469). Here we have tested again AUDI2000 together with 11 advanced colour-difference formulas (CIELUV, CIELAB, CMC, BFD, CIE94, CIEDE2000, CAM02-UCS, CAM02-SCD, DIN99d, DIN99b, OSA-GP-Euclidean) for three visual datasets we may consider particularly useful to the automotive industry because of different reasons: 1) 828 metallic colour pairs used to develop the highly reliable RIT-DuPont dataset (Color Res. Appl. 35, 274-283, 2010); 2) printed samples conforming 893 colour pairs with threshold colour differences (J. Opt. Soc. Am. A 29, 883-891, 2012); 3) 150 colour pairs in a tolerance dataset proposed by AUDI. To measure the relative merits of the different tested colour-difference formulas, we employed the STRESS index (J. Opt. Soc. Am. A 24, 1823-1829, 2007), assuming a 95% confidence level. For datasets 1) and 2), AUDI2000 was in the group of the best colour-difference formulas with no significant differences with respect to CIE94, CIEDE2000, CAM02-UCS, DIN99b and DIN99d formulas. For dataset 3) AUDI2000 provided the best results, being statistically significantly better than all other tested colour-difference formulas.

  13. Effects of watershed densities of animal feeding operations on nutrient concentrations and estrogenic activity in agricultural streams.

    PubMed

    Ciparis, Serena; Iwanowicz, Luke R; Voshell, J Reese

    2012-01-01

    Application of manures from animal feeding operations (AFOs) as fertilizer on agricultural land can introduce nutrients and hormones (e.g. estrogens) to streams. A landscape-scale study was conducted in the Shenandoah River watershed (Virginia, USA) in order to assess the relationship between densities of AFOs in watersheds of agricultural streams and in-stream nutrient concentrations and estrogenic activity. The effect of wastewater treatment plants (WWTPs) on nutrients and estrogenic activity was also evaluated. During periods of high and low flow, dissolved inorganic nitrogen (DIN) and orthophosphate (PO(4)-P) concentrations were analyzed and estrogens/estrogenic compounds were extracted and quantified as17β-estradiol equivalents (E2Eq) using a bioluminescent yeast estrogen screen. Estrogenic activity was measurable in the majority of collected samples, and 20% had E2Eq concentrations >1 ng/L. Relatively high concentrations of DIN (>1000 μg/L) were also frequently detected. During all sampling periods, there were strong relationships between watershed densities of AFOs and in-stream concentrations of DIN (R(2) = 0.56-0.81) and E2Eq (R(2) = 0.39-0.75). Relationships between watershed densities of AFOs and PO(4)-P were weaker, but were also significant (R(2) = 0.27-0.57). When combined with the effect of watershed AFO density, streams receiving WWTP effluent had higher concentrations of PO(4)-P than streams without WWTP discharges, and PO(4)-P was the only analyte with a consistent relationship to WWTPs. The results of this study suggest that as the watershed density of AFOs increases, there is a proportional increase in the potential for nonpoint source pollution of agricultural streams and their receiving waters by nutrients, particularly DIN, and compounds that can cause endocrine disruption in aquatic organisms. PMID:22088420

  14. Christopher Lasch and Prairie Populism

    ERIC Educational Resources Information Center

    Lauck, Jon K.

    2012-01-01

    Christopher Lasch was born in Omaha in 1932. By the end of his life, cut short at age sixty-one, he had become one of the most famous intellectuals in the world. During his life of active writing from the time of the early Cold War until the fall of the Soviet Union, Lasch's distinctive voice pierced through the din of the nation's noisy political…

  15. Characterization of quantum circulant networks having perfect state transfer

    NASA Astrophysics Data System (ADS)

    Bašić, Milan

    2013-01-01

    In this paper we answer the question of when circulant quantum spin networks with nearest-neighbor couplings can give perfect state transfer. The network is described by a circulant graph G, which is characterized by its circulant adjacency matrix A. Formally, we say that there exists a perfect state transfer (PST) between vertices {a,bin V(G)} if | F( τ) ab | = 1, for some positive real number τ, where F( t) = exp(i At). Saxena et al. (Int J Quantum Inf 5:417-430, 2007) proved that | F( τ) aa | = 1 for some {ain V(G)} and {tauin {R}^+} if and only if all eigenvalues of G are integer (that is, the graph is integral). The integral circulant graph ICG n ( D) has the vertex set Z n = {0, 1, 2, . . . , n - 1} and vertices a and b are adjacent if {gcd(a-b,n)in D} , where {D subseteq {d : d mid n, 1 ≤ d < n}} . These graphs are highly symmetric and have important applications in chemical graph theory. We show that ICG n ( D) has PST if and only if {nin 4{N}} and {D=widetilde{D_3} \\cup D_2\\cup 2D_2\\cup 4D_2|cup {n/2^a}} , where {widetilde{D_3}={din D | n/din 8{N}}, D_2= {din D | n/din 8{N}+4}{setminus}{n/4}} and {ain{1,2}} . We have thus answered the question of complete characterization of perfect state transfer in integral circulant graphs raised in Angeles-Canul et al. (Quantum Inf Comput 10(3&4):0325-0342, 2010). Furthermore, we also calculate perfect quantum communication distance (distance between vertices where PST occurs) and describe the spectra of integral circulant graphs having PST. We conclude by giving a closed form expression calculating the number of integral circulant graphs of a given order having PST.

  16. Khayyam [al-Khayyam], Omar [Abu ol-Fath ebn-Ebrahim `Omar ol-Khayyami of Nishapur] (c. 1044-c. 1123)

    NASA Astrophysics Data System (ADS)

    Murdin, P.

    2000-11-01

    Poet, mathematician, astronomer, born in Nishapur, Persia (now Iran), to a family of Arabic tent makers (a translation of his name), an outstanding mathematician and astronomer. The Saljuq Sultan, Malikshah Jalal al-Din, appointed him to the observatory at Ray around 1074 to determine the solar calendar, as an aid to tax collection. Khayyam developed a calendar called Al-Tarikh-al-Jalali, which h...

  17. Dissolved inorganic P and N input into the Yangtze estuary: ecological response

    NASA Astrophysics Data System (ADS)

    Xu, H.

    2013-12-01

    The Yangtze estuary has received more environmental hazards in the past decades. For instance, the occurrences of red tides have increased from 8 times to >100 times from 1980s to 2000s. Large-scale blooming can cover an estuarine water area of >1000 km2. There are many factors that can drive the algae blooming, such as temperature, illumination, salinity and nutrients flux, etc. However, it seems that the riverine dissolved inorganic phosphate (DIP) and nitrogen (DIN) has been increased dramatically over the last half-century in the Yangtze estuary. Therefore, the DIP and DIN are thought to be the predominant factor in deteriorating estuarine eco-health. This study has calculated the annual DIP into the Yangtze estuary, increasing from 7.6 x 103 ton in 1972 to 35 x 103 ton in 2007, and DIN from 0.2 x 106 ton to 1.33 x 106 ton in the same period. Our evidence shows that the riverine nutrients were dominated by fertilizer in decades ago, but it has been changed to domestic sewage since 2000. Overloading nutrients have directly changed the algae species in the estuarine waters, i.e. from Skeletonema costatum to Prorocentrum donghaiens, which enlarges the time duration and distribution scale of algae blooming. Sediment concentration which can absorb considerable DIP and DIN, is to be largely lessened in the estuarine waters due to the Three-Gorges damming. In this study, we are applying the muddy-LOICZ model to help estimate the role of SSC for nutrients distribution after damming. Our preliminary results have proved ca. 20% DIP that will increase in response to reduction of SSC in future. This will exert a significant impact on the ecosystem of the Yangtze estuary. The results would provide insight to a sound coastal water management.

  18. Benthic nutrient fluxes in the Eastern Gotland Basin (Baltic Sea) with particular focus on microbial mat ecosystems

    NASA Astrophysics Data System (ADS)

    Noffke, A.; Sommer, S.; Dale, A. W.; Hall, P. O. J.; Pfannkuche, O.

    2016-06-01

    Benthic fluxes and water column distributions of dissolved inorganic nitrogen (DIN) and total dissolved phosphate (PO43 -) were measured in situ at 7 sites across a redox gradient from oxic to anoxic bottom waters in the Eastern Gotland Basin (Baltic Sea). The study area was divided into the oxic zone (60 to ca. 80 m water depth, O2 > 30 μM), the hypoxic transition zone (HTZ, ca. 80 to 120 m, O2 < 30 μM) and the deep anoxic and sulfidic basin (> ca. 120 m). Sediments in the HTZ were covered by mats of vacuolated sulfur bacteria. Ammonium (NH4+) fluxes in the deep basin and the HTZ were elevated at 0.6 mmol m- 2 d- 1 and 1 mmol m- 2 d- 1, respectively. Nitrate (NO3-) fluxes were directed into the sediment at all stations in the HTZ and were zero in the deep basin. PO43 - release was highest in the HTZ at 0.23 mmol m- 2 d- 1, with a further release of 0.2 mmol m- 2 d- 1 in the deep basin. Up-scaling the benthic fluxes to the Baltic Proper equals 109 kt yr- 1 of PO43 - and 266 kt yr- 1 of DIN. This is eight- and two-fold higher than the total external load of P (14 kt yr- 1) and DIN (140 kt yr- 1) in 2006 (HELCOM 2009b). The HTZ makes an important contribution to the internal nutrient loading in the Baltic Proper, releasing 70% of P (76 kt yr- 1) and 75% of DIN (200 kt yr- 1) despite covering only 51% of area.

  19. Using the soil and water assessment tool to estimate dissolved inorganic nitrogen water pollution abatement cost functions in central portugal.

    PubMed

    Roebeling, P C; Rocha, J; Nunes, J P; Fidélis, T; Alves, H; Fonseca, S

    2014-01-01

    Coastal aquatic ecosystems are increasingly affected by diffuse source nutrient water pollution from agricultural activities in coastal catchments, even though these ecosystems are important from a social, environmental and economic perspective. To warrant sustainable economic development of coastal regions, we need to balance marginal costs from coastal catchment water pollution abatement and associated marginal benefits from coastal resource appreciation. Diffuse-source water pollution abatement costs across agricultural sectors are not easily determined given the spatial heterogeneity in biophysical and agro-ecological conditions as well as the available range of best agricultural practices (BAPs) for water quality improvement. We demonstrate how the Soil and Water Assessment Tool (SWAT) can be used to estimate diffuse-source water pollution abatement cost functions across agricultural land use categories based on a stepwise adoption of identified BAPs for water quality improvement and corresponding SWAT-based estimates for agricultural production, agricultural incomes, and water pollution deliveries. Results for the case of dissolved inorganic nitrogen (DIN) surface water pollution by the key agricultural land use categories ("annual crops," "vineyards," and "mixed annual crops & vineyards") in the Vouga catchment in central Portugal show that no win-win agricultural practices are available within the assessed BAPs for DIN water quality improvement. Estimated abatement costs increase quadratically in the rate of water pollution abatement, with largest abatement costs for the "mixed annual crops & vineyards" land use category (between 41,900 and 51,900 € tDIN yr) and fairly similar abatement costs across the "vineyards" and "annual crops" land use categories (between 7300 and 15,200 € tDIN yr). PMID:25602550

  20. Surface deformation of Taipei basin detected by Differential SAR Interferometry

    NASA Astrophysics Data System (ADS)

    Chen, Y.; Chang, C.; Yen, J.; Lin, M.

    2006-12-01

    Taiwan island is located between the southeastern periphery of the Eurasian plate and the Philippine Sea plate. The two converging plates produced very active tectonics, and can be seen by the high seismicity and deformation rate. Taipei, the highest populated area, center of politics, and economics in Taiwan, is in Taipei basin at the northern part of the island. There are several faults in and surrounding the basin, and the city is threatened with a high geological hazard potential that we should keep monitoring the crustal deformation to prevent and mitigate the disaster effect. The aims of our study is to apply the DInSAR technique to determine the surface deformation of Taipei basin area, and discussing the relation between the manifestation of deformation and the tectonically active region, Shanjiao fault. In the past few years, Differential SAR Interferometry (DInSAR) has been proved to be a powerful technique for monitoring the neotectonic activities and natural hazards. High spatial sampling rate of DInSAR technique allows studies of surface deformations with centimeter accuracy. In this area, we used ERS-1/2 SAR images acquired from 1993 to 2005 to generate 10 differential interferograms and processed the data using DIAPASON developed by CNES and SRTM global DEM.From our results, the deformation rate in Taipei is generally high in the western end of the basin along the Shanjiao fault and decrease eastward, while the subsidence center often appeared in the center of the Taipei basin. The neotectonic activity of the Shanjiao fault appeared to be insignificant by itself but it seemed to separate the subsiding basin from the surrounding areas. Further comparison between our DInSAR results and isopach of the Taipei basin revealed that the subsidence centers appeared in the interferograms did not coincide with the location where the sediments are thickest. Our results from differential interferometry will be compared to other geodetic measurements such as the

  1. Effects of watershed densities of animal feeding operations on nutrient concentrations and estrogenic activity in agricultural streams

    USGS Publications Warehouse

    Ciparis, S.; Iwanowicz, L.R.; Voshell, J.R.

    2012-01-01

    Application of manures from animal feeding operations (AFOs) as fertilizer on agricultural land can introduce nutrients and hormones (e.g. estrogens) to streams. A landscape-scale study was conducted in the Shenandoah River watershed (Virginia, USA) in order to assess the relationship between densities of AFOs in watersheds of agricultural streams and in-stream nutrient concentrations and estrogenic activity. The effect of wastewater treatment plants (WWTPs) on nutrients and estrogenic activity was also evaluated. During periods of high and low flow, dissolved inorganic nitrogen (DIN) and orthophosphate (PO 4-P) concentrations were analyzed and estrogens/estrogenic compounds were extracted and quantified as17??-estradiol equivalents (E2Eq) using a bioluminescent yeast estrogen screen. Estrogenic activity was measurable in the majority of collected samples, and 20% had E2Eq concentrations >1ng/L. Relatively high concentrations of DIN (>1000??g/L) were also frequently detected. During all sampling periods, there were strong relationships between watershed densities of AFOs and in-stream concentrations of DIN (R 2=0.56-0.81) and E2Eq (R 2=0.39-0.75). Relationships between watershed densities of AFOs and PO 4-P were weaker, but were also significant (R 2=0.27-0.57). When combined with the effect of watershed AFO density, streams receiving WWTP effluent had higher concentrations of PO 4-P than streams without WWTP discharges, and PO 4-P was the only analyte with a consistent relationship to WWTPs. The results of this study suggest that as the watershed density of AFOs increases, there is a proportional increase in the potential for nonpoint source pollution of agricultural streams and their receiving waters by nutrients, particularly DIN, and compounds that can cause endocrine disruption in aquatic organisms. ?? 2011 Elsevier B.V.

  2. Effects of watershed densities of animal feeding operations on nutrient concentrations and estrogenic activity in agricultural streams

    USGS Publications Warehouse

    Ciparis, Serena; Iwanowicz, Luke R.; Voshell, J. Reese

    2012-01-01

    Application of manures from animal feeding operations (AFOs) as fertilizer on agricultural land can introduce nutrients and hormones (e.g. estrogens) to streams. A landscape-scale study was conducted in the Shenandoah River watershed (Virginia, USA) in order to assess the relationship between densities of AFOs in watersheds of agricultural streams and in-stream nutrient concentrations and estrogenic activity. The effect of wastewater treatment plants (WWTPs) on nutrients and estrogenic activity was also evaluated. During periods of high and low flow, dissolved inorganic nitrogen (DIN) and orthophosphate (PO4-P) concentrations were analyzed and estrogens/estrogenic compounds were extracted and quantified as17β-estradiol equivalents (E2Eq) using a bioluminescent yeast estrogen screen. Estrogenic activity was measurable in the majority of collected samples, and 20% had E2Eq concentrations > 1 ng/L. Relatively high concentrations of DIN (> 1000 μg/L) were also frequently detected. During all sampling periods, there were strong relationships between watershed densities of AFOs and in-stream concentrations of DIN (R2 = 0.56–0.81) and E2Eq (R2 = 0.39–0.75). Relationships between watershed densities of AFOs and PO4-P were weaker, but were also significant (R2 = 0.27–0.57). When combined with the effect of watershed AFO density, streams receiving WWTP effluent had higher concentrations of PO4-P than streams without WWTP discharges, and PO4-P was the only analyte with a consistent relationship to WWTPs. The results of this study suggest that as the watershed density of AFOs increases, there is a proportional increase in the potential for nonpoint source pollution of agricultural streams and their receiving waters by nutrients, particularly DIN, and compounds that can cause endocrine disruption in aquatic organisms.

  3. Linking benthic community structure to terrestrial runoff and upwelling in the coral reefs of northeastern Hainan Island

    NASA Astrophysics Data System (ADS)

    Li, Xiubao; Wang, Daoru; Huang, Hui; Zhang, Jing; Lian, Jiansheng; Yuan, Xiangcheng; Yang, Jianhui; Zhang, Guoseng

    2015-04-01

    Near-shore coral reefs in northeastern Hainan Island are close to river mouths and aquaculture ponds, and also located at the center of the Qiongdong Upwelling (QDU). However, it is still unclear how terrestrial runoff and upwelling influence the community composition and spatial distribution of the benthos. During three cruises in 2010 and 2011 in Wenchang, northeastern Hainan Island, we determined a subset of environmental parameters in seawater (e.g. temperature, salinity, DO, dissolved inorganic nutrient (DIN), turbidity and transparency) and macroalgal δ15N and investigated the benthic communities (e.g. live coral cover, coral species richness, juvenile coral density, macroalgal cover and coverage of calcified algae) by video transect and visual census techniques at 10 stations (i.e. 1S-6D). The results showed that the QDU has influenced the reef waters in Wenchang. In 2011, the upwelling started in early May, peaked in July and disappeared in September and most upwelling events lasted for 1-2 weeks between May and July. The results also demonstrated that the reef water was nutrient enriched. Stations close to the river mouth and aquaculture ponds had higher levels of DIN and a higher percentage of ammonia in DIN, and there was consistently lower live coral cover, juvenile coral density and higher macroalgal cover. At some stations in this study, live coral cover was negatively correlated with macroalgal cover (i.e. 2S-6D). Live coral cover, species richness, and juvenile coral density all increased with the distance away from the river outlet and decreased with the rise of DIN. These results suggest that terrestrial runoff and upwelling stimulate nutrient enrichment, and that overgrowing macroalgae has an important influence on the coral communities in northeastern Hainan Island.

  4. Different contributions of riverine and oceanic nutrient fluxes supporting primary production in Ishikari Bay

    NASA Astrophysics Data System (ADS)

    Agboola, Julius I.; Kudo, Isao

    2014-10-01

    We computed a ratio of riverine nutrient flux (RNF) to bottom nutrient flux (BNF) to determine the relative importance of oceanic and riverine nutrient fluxes on primary production dynamics in Ishikari Bay, which is composed of oligotrophic subarctic coastal water. Across spring, summer and autumn, the RNF:BNF ratio (R:B ratio) was significantly greater than 1.0, especially in spring and autumn for DIN and Si(OH)4, suggesting that riverine nutrients mostly supported primary production. A strong inverse relationship (r=-0.927) between Chl a and salinity in autumn and a corresponding increase in the apparent utilization of DIN and primary production indicated that the contribution of DIN from the Ishikari River on primary production was maximal in autumn. However the R:B ratio for PO4 was significantly less than 1.0, especially in summer (0.1) and autumn (0.3), suggesting a larger contribution of bottom upwelling nutrient sources. In spring, when the ratio was close to 1 (0.8), PO4 supply from both bottom (upwelling) and surface (river) was equivalent, since PO4 concentration of river end-member was the lowest. Although riverine nutrient fluxes were a major source of DIN and Si(OH)4 nutrient supply in the bay, oceanic nutrient contribution from bottom upwelling and horizontal advection was a major source of PO4. While riverine nutrients significantly fuel primary production, the estuarine circulation process may contribute significantly to compensating for the inadequate supply of riverine PO4 in an oligotrophic system like Ishikari Bay. Also, unlike the usual estuarine system in which nutrient concentration at a deeper layer is high due to the regeneration of nutrients at depth, concentration in Ishikari Bay was very low due to an influence of oligotrophic waters. We conclude that riverine nutrient flux contributes a large portion of the total flux in Ishikari Bay.

  5. Entropía de la información: una herramienta útil

    NASA Astrophysics Data System (ADS)

    Cincotta, P.

    En este trabajo se presenta una muy breve introducción al concepto de Entropía de la Información y se muestran distintas aplicaciones de una misma técnica para estudiar problemas tan variados como la determinación de periodicidad en una serie de tiempo arbitraria o de estimar el grado de caoticidad de una trayectoria en un sistema dinámico de N grados de libertad.

  6. Creation Of The Residual Stress By Influence Of Wear Of Cutting Tool And Their Analysis

    NASA Astrophysics Data System (ADS)

    Kordík, Marek; Čilliková, Mária; Mrazik, Jozef; Martinček, Juraj; Janota, Miroslav; Nicielnik, Henryk

    2015-12-01

    The aim of this paper is analysis of turned bearing ring made of material 14109 (DIN 100Cr6) without heat treatment. For the analysis a mechanical destructive method was chosen. Analysis focused on existence and character of residual stresses after turning operation of bearing ring by tool with different level of wear. The experiment reveals the relationships between residual stress creation and cutting tool wear.

  7. SAR interferometry and optical remote sensing for analysis of co-seismic deformation, source characteristics and mass wasting pattern of Lushan (China, April 2013) earthquake

    NASA Astrophysics Data System (ADS)

    Mathew, John; Majumdar, Ritwik; Kumar, K. Vinod

    2015-03-01

    Co-seismic deformation associated with the Lushan (China) earthquake that occurred along the south-western segment of the Longmenshan Fault Zone (LFZ) on the 20th April 2013 has been estimated by differential interferometric SAR (DInSAR) technique using Radarsat-2 data. The Lushan earthquake resulted in the deformation of the Sichuan basin and the Longmenshan ranges in proximity to the LFZ. The line of sight (LOS) displacement values obtained from DInSAR technique mainly range between -4.0 cm to +3.0 cm. The western Sichuan basin shows oblique westward movement with predominant downward component in areas farther from LFZ and predominant westward component over the downward movement in areas closer to the source fault. Inversion modelling has been used to derive the seismic source characteristics from DInSAR derived deformation values using elastic dislocation source type. The linear inversion model converged at a double-fault source solution consisting of a deeper, steep, NW dipping fault plane-1 of 60 km × 16 km dimension and a shallower, gentle, NW dipping fault plane-2 of 60 km × 15 km dimension, with distributed slip values varying between 0 to 2.26 m. These fault planes (fault planes-1 and -2) coincide with the Dachuan-Shuangshi fault and the buried Range Front Fault, respectively. The inversion model gives a moment magnitude of 6.81 and the geodetic moment of 2.07 × 1019 Nm, comparable to those given in literature, derived using teleseismic body wave data. Thus DInSAR technique helped to quantify the co-seismic deformation and to retrieve the source characteristics from the estimated deformation values. The study also evaluated the distribution pattern of earthquake induced landslides (EIL) triggered fresh or re-activated during the Lushan earthquake and found that they show spatial association with the seismic source zone and also with various pre-conditioning factors of slope instability.

  8. Dissolved inorganic nitrogen pools and surface flux under different brackish marsh vegetation types, common reed (Phragmites australis) and salt hay (Spartina patens)

    USGS Publications Warehouse

    Windham-Myers, L.

    2005-01-01

    The current expansion of Phragmites australis into the high marsh shortgrass (Spartina patens, Distichlis spicata) communities of eastern U.S. salt marshes provided an opportunity to identify the influence of vegetation types on pools and fluxes of dissolved inorganic nitrogen (DIN). Two brackish tidal marshes of the National Estuarine Research Reserve system were examined, Piermont Marsh of the Hudson River NERR in New York and Hog Island in the Jacques Coustaeu NERR of New Jersey. Pools of DIN in porewater and rates of DIN surface flux were compared in replicated pairs of recently-expanded P. australis and neighboring S. patens-dominated patches on the high marsh surface. Both marshes generally imported nitrate (NO3-) and exported ammonium (NH4+), such that overall DIN was exported. No differences in surface exchange of NO3- or NH4+ were observed between vegetation types. Depth-averaged porewater NH4+ concentrations over the entire growing season were 56% lower under P. australis than under S. patens (average 1.4 vs. 3.2 mg NH4+ L-1) with the most profound differences in November. Porewater profiles showed an accumulation of NH4+ at depth in S. patens and constant low concentrations in P. australis from the soil surface to 50 cm depth, with no significant differences in porewater salinity. Despite these profound differences in porewater, NH 4+ diffusion from soils of P. australis and S. patens were not measurably different, were similar to other published rates, and were well below estimated rates based on passive diffusion alone. Rapid adsorption and uptake by litter and microbes in surface soils of both communities may buffer NH4+ loss to flooding tides in both communities, thereby reducing the impact of P. australis invasion on NH4+ flux to flooding waters. ?? Springer 2005.

  9. Structural basis for the blockade of MATE multidrug efflux pumps

    DOE PAGESBeta

    Radchenko, Martha; Symersky, Jindrich; Nie, Rongxin; Lu, Min

    2015-08-06

    Multidrug and toxic compound extrusion (MATE) transporters underpin multidrug resistance by using the H+ or Na+ electrochemical gradient to extrude different drugs across cell membranes. MATE transporters can be further parsed into the DinF, NorM and eukaryotic subfamilies based on their amino-acid sequence similarity. Here we report the 3.0 Å resolution X-ray structures of a protonation-mimetic mutant of an H+-coupled DinF transporter, as well as of an H+-coupled DinF and a Na+-coupled NorM transporters in complexes with verapamil, a small-molecule pharmaceutical that inhibits MATE-mediated multidrug extrusion. Combining structure-inspired mutational and functional studies, we confirm the biological relevance of our crystalmore » structures, reveal the mechanistic differences among MATE transporters, and suggest how verapamil inhibits MATE-mediated multidrug efflux. Our findings offer insights into how MATE transporters extrude chemically and structurally dissimilar drugs and could inform the design of new strategies for tackling multidrug resistance.« less

  10. Active landslides detection at regional scale: comparing multitemporal InSAR with "handmade" inventories

    NASA Astrophysics Data System (ADS)

    Derron, Marc-Henri; Michoud, Clément; Jaboyedoff, Michel; Runne Lauknes, Tom

    2013-04-01

    Advanced multitemporal differential SAR interferometry has proved to be able to detect unexpected active landslides in areas difficult to access. That makes this technique very useful to complete inventories. But that does not mean that applying A-DInSAR is a sufficient tool to build an inventory. Some A-DInSAR results have been compared in areas where independent landslides inventories exists, in Norway (J. Dehls at the Geological Survey of Norway) and Italy (A. Tamburini, TRE Milano). Roughly, depending on the surface cover, geomorphology and images availability, from 0 to 50% of the already known landslides can be detected with A-DInSAR results. We are testing a similar approach in Switzerland, in the county of Vaud where two landslides inventories are available (DUTI 1985, Jaboyedoff 2009). A set of 24 Envisat ASAR images (single frame/ single track) have been processed with PS-INSAR and SBAS techniques. Because of the large variety of surface cover (urban, forested, rocky) and morphology (plane and mountainous areas), both techniques provides very different results depending on local conditions.

  11. Net Subterranean Estuarine Export Fluxes of Dissolved Inorganic C, N, P, Si, and Total Alkalinity into the Jiulong River Estuary, China

    NASA Astrophysics Data System (ADS)

    Wang, G.; Wang, Z.; Zhai, W. D.; Moore, W. S.; Li, Q.; Yan, X.; Qi, D.; Jiang, Y.

    2014-12-01

    To evaluate geochemical impacts of the subterranean estuary (STE) on the Jiulong River estuary, China, we estimated seasonal fluxes of subterranean water discharge into the estuary based on the mass balance of radium isotopes and net subterranean export fluxes of dissolved inorganic C (DIC), N (DIN), Si (DSi), soluble reactive phosphorus (SRP), and total alkalinity (TA). Based on 226Ra data, the subterranean discharge (in 107 m3 d-1) was estimated to be 0.24~0.51 in the spring, 0.56~1.16 in the summer, 0.38~0.79 in the fall, and 0.22~0.45 in the winter. This was equivalent to 6-16% of the concomitant river discharge. The net spatially integrated material fluxes from the STE into the estuary were equivalent up to 51-89% of the concomitant riverine fluxes for DIC and TA, around 10-25% for DSi and DIN, and negligible for SRP. Paradoxically, the mixing lines along the salinity gradient revealed no apparent additions of these species. These additions are not revealed because the STE is a relatively small spatially-averaged source that spreads throughout the estuary in contrast to the major point sources of the river and the ocean for the estuary. Thus, despite apparent conservative mixing of DIC, DIN, and DSi, subterranean exports of these species into estuaries must be taken into account in evaluating geochemical impacts of estuarine exports on shelf waters.

  12. The transcription elongation factor NusA is required for stress-induced mutagenesis in Escherichia coli.

    PubMed

    Cohen, Susan E; Walker, Graham C

    2010-01-12

    Stress-induced mutagenesis describes the accumulation of mutations that occur in nongrowing cells, in contrast to mutagenesis that occurs in actively dividing populations, and has been referred to as stationary-phase or adaptive mutagenesis. The most widely studied system for stress-induced mutagenesis involves monitoring the appearance of Lac(+) revertants of the strain FC40 under starvation conditions in Escherichia coli. The SOS-inducible translesion DNA polymerase DinB plays an important role in this phenomenon. Loss of DinB (DNA pol IV) function results in a severe reduction of Lac(+) revertants. We previously reported that NusA, an essential component of elongating RNA polymerases, interacts with DinB. Here we report our unexpected observation that wild-type NusA function is required for stress-induced mutagenesis. We present evidence that this effect is unlikely to be due to defects in transcription of lac genes but rather is due to an inability to adapt and mutate in response to environmental stress. Furthermore, we extended our analysis to the formation of stress-induced mutants in response to antibiotic treatment, observing the same striking abolition of mutagenesis under entirely different conditions. Our results are the first to implicate NusA as a crucial participant in the phenomenon of stress-induced mutagenesis. PMID:20036541

  13. Lack of nitrogen as a causing agent of Cylindrospermopsis raciborskii intermittent blooms in a small tropical reservoir.

    PubMed

    Figueredo, Cleber C; von Rückert, Gabriela; Cupertino, Arthur; Pontes, Marília A; Fernandes, Luyara A; Ribeiro, Solange G; Maran, Natália R C

    2014-03-01

    Cylindrospermopsis raciborskii is the focus of many studies due to its toxicity and increasing blooms frequency. The comprehension of the factors that might trigger these blooms is fundamental for the maintenance of good freshwater quality. To better understand the autoecology of C. raciborskii and to identify the factors controlling its dominance, general limnological features were evaluated in Pedalinhos reservoir, Brazil. Samplings were performed monthly between 2011 and 2013. Although C. raciborskii is considered a relatively perennial species in the tropics, it presented an interesting nonunimodal variation, with biovolume varying from 0 to c. 30 mm³ L⁻¹ in short time intervals (< 30 days). These temporal trends allowed the evaluation of the isolated effects of nonclimatic variables. Cylindrospermopsis raciborskii was connected with several variables, but nitrogen was the major determinant of its dynamics. Blooms were observed when dissolved inorganic nitrogen (DIN) was scarce (< 50 μg L⁻¹), mainly in relation to the availability of phosphorus and total-N (DIN : total-P < 3 and DIN : total-N < 0.04). This paper contributes to establish strategies to avoid C. raciborskii blooms, suggesting that a management in P levels would be not sufficient. It is necessary to consider N availability to avoid its dominance in the reservoir. PMID:24329601

  14. Atmospheric input of inorganic nitrogen and phosphorus to the Ligurian Sea: Data from the Cap Ferrat coastal time-series station

    NASA Astrophysics Data System (ADS)

    de Fommervault, Orens Pasqueron; Migon, Christophe; Dufour, Aurélie; D'Ortenzio, Fabrizio; Kessouri, Fayçal; Raimbault, Patrick; Garcia, Nicole; Lagadec, Véronique

    2015-12-01

    Atmospheric fluxes of bio-available inorganic nitrogen (DIN, i.e. nitrate+ammonium) and phosphorus (DIP, i.e. phosphate) were measured in 2010, 2011 and 2013 at the sampling station of Cap Ferrat (Ligurian Sea). Wet and dry fluxes of DIN, averaged over three years, were 35 and 19 mmol m-2 yr-1, respectively. Most of the nitrate was deposited under dry form, whilst ammonia was twice more found in wet deposition. Wet and dry fluxes of DIP, averaged over three years, were 0.11 and 0.64 mmol m-2 yr-1, respectively. Atmospheric fluxes of DIN and DIP were compared with other photic zone nutrient input sources, physical and biological, i.e. winter convection, N2 fixation, and upward diffusion. Even if convection is by far the most important nutrient input for surface waters, atmospheric sources may be the second one, supplying more nutrients than diazotrophy and diffusion, particularly in conditions of water column stratification.

  15. A-Differential Synthetic Aperture Radar Interferometry analysis of a Deep Seated Gravitational Slope Deformation occurring at Bisaccia (Italy).

    PubMed

    Di Martire, Diego; Novellino, Alessandro; Ramondini, Massimo; Calcaterra, Domenico

    2016-04-15

    This paper presents the results of an investigation on a Deep Seated Gravitational Slope Deformation (DSGSD), previously only hypothesized by some authors, affecting Bisaccia, a small town located in Campania region, Italy. The study was conducted through the integration of conventional methods (geological-geomorphological field survey, air-photo interpretation) and an Advanced-Differential Interferometry Synthetic Aperture Radar (A-DInSAR) technique. The DSGSD involves a brittle lithotype (conglomerates of the Ariano Irpino Supersynthem) resting over a Structurally Complex Formation (Varycoloured Clays of Calaggio Formation). At Bisaccia, probably as a consequence of post-cyclic recompression phenomena triggered by reiterated seismic actions, the rigid plate made up of conglomeratic sediments resulted to be split in five portions, showing different rates of displacements, whose deformations are in the order of some centimeter/year, thus inducing severe damage to the urban settlement. A-DInSAR techniques confirmed to be a reliable tool in monitoring slow-moving landslides. In this case 96 ENVIronmental SATellite-Advanced Synthetic Aperture Radar (ENVISAT-ASAR) images, in ascending and descending orbits, have been processed using SUBSOFT software, developed by the Remote Sensing Laboratory (RSLab) group from the Universitat Politècnica de Catalunya (UPC). The DInSAR results, coupled with field survey, supported the analysis of the instability mechanism and confirmed the historical record of the movements already available for the town. PMID:26849321

  16. Global river nutrient export: A scenario analysis of past and future trends

    NASA Astrophysics Data System (ADS)

    Seitzinger, S. P.; Mayorga, E.; Bouwman, A. F.; Kroeze, C.; Beusen, A. H. W.; Billen, G.; van Drecht, G.; Dumont, E.; Fekete, B. M.; Garnier, J.; Harrison, J. A.

    2010-12-01

    An integrated modeling approach was used to connect socioeconomic factors and nutrient management to river export of nitrogen, phosphorus, silica and carbon based on an updated Global NEWS model. Past trends (1970-2000) and four future scenarios were analyzed. Differences among the scenarios for nutrient management in agriculture were a key factor affecting the magnitude and direction of change of future DIN river export. In contrast, connectivity and level of sewage treatment and P detergent use were more important for differences in DIP river export. Global particulate nutrient export was calculated to decrease for all scenarios, in part due to increases in dams for hydropower. Small changes in dissolved silica and dissolved organics were calculated for all scenarios at the global scale. Population changes were an important underlying factor for river export of all nutrients in all scenarios. Substantial regional differences were calculated for all nutrient elements and forms. South Asia alone accounted for over half of the global increase in DIN and DIP river export between 1970 and 2000 and in the subsequent 30 years under the Global Orchestration scenario (globally connected with reactive approach to environmental problems); DIN river export decreased in the Adapting Mosaic (globally connected with proactive approach) scenario by 2030, although DIP continued to increase. Risks for coastal eutrophication will likely continue to increase in many world regions for the foreseeable future due to both increases in magnitude and changes in nutrient ratios in river export.

  17. Changes in nitrogen and phosphorus and their effects on phytoplankton in the Bohai Sea

    NASA Astrophysics Data System (ADS)

    Xu, Sisi; Song, Jinming; Li, Xuegang; Yuan, Huamao; Li, Ning; Duan, Liqin; Sun, Peiyan

    2010-07-01

    Systematic studies of the changes in dissolved inorganic nitrogen (DIN) and dissolved inorganic phosphorus (DIP) and their effects on phytoplankton over the last 30 years in the Bohai Sea are presented. The amount of sewage disposal, use of fertilizer and the Huanghe River runoff were found to have a significant influence on the DIN or DIP concentrations in the Bohai Sea over the last 30 years. Moreover, the changes in DIN and DIP resulted in changes in the limiting nutrients of phytoplankton in the Bohai Sea from nitrogen in the early 1980s to nitrogen-phosphorus in the late 1980s, and then to phosphorus after the 1990s. In addition, changes in nitrogen and phosphorus had a significant effect on the phytoplankton community structure. The half saturation constant ( K s) was used to evaluate the effect of nutrients on the phytoplankton community structure in the Bohai Sea over the last 30 years. Cell abundance percentages of dominant phytoplankton species with high K s values for phosphorus and low K s values for nitrogen have decreased since the 1980s, while those of dominant phytoplankton species with low K s values for phosphorus and high K s values for nitrogen increased during this period.

  18. Structural basis for the blockade of MATE multidrug efflux pumps

    SciTech Connect

    Radchenko, Martha; Symersky, Jindrich; Nie, Rongxin; Lu, Min

    2015-08-06

    Multidrug and toxic compound extrusion (MATE) transporters underpin multidrug resistance by using the H+ or Na+ electrochemical gradient to extrude different drugs across cell membranes. MATE transporters can be further parsed into the DinF, NorM and eukaryotic subfamilies based on their amino-acid sequence similarity. Here we report the 3.0 Å resolution X-ray structures of a protonation-mimetic mutant of an H+-coupled DinF transporter, as well as of an H+-coupled DinF and a Na+-coupled NorM transporters in complexes with verapamil, a small-molecule pharmaceutical that inhibits MATE-mediated multidrug extrusion. Combining structure-inspired mutational and functional studies, we confirm the biological relevance of our crystal structures, reveal the mechanistic differences among MATE transporters, and suggest how verapamil inhibits MATE-mediated multidrug efflux. Our findings offer insights into how MATE transporters extrude chemically and structurally dissimilar drugs and could inform the design of new strategies for tackling multidrug resistance.

  19. Structural basis for the blockade of MATE multidrug efflux pumps

    NASA Astrophysics Data System (ADS)

    Radchenko, Martha; Symersky, Jindrich; Nie, Rongxin; Lu, Min

    2015-08-01

    Multidrug and toxic compound extrusion (MATE) transporters underpin multidrug resistance by using the H+ or Na+ electrochemical gradient to extrude different drugs across cell membranes. MATE transporters can be further parsed into the DinF, NorM and eukaryotic subfamilies based on their amino-acid sequence similarity. Here we report the 3.0 Å resolution X-ray structures of a protonation-mimetic mutant of an H+-coupled DinF transporter, as well as of an H+-coupled DinF and a Na+-coupled NorM transporters in complexes with verapamil, a small-molecule pharmaceutical that inhibits MATE-mediated multidrug extrusion. Combining structure-inspired mutational and functional studies, we confirm the biological relevance of our crystal structures, reveal the mechanistic differences among MATE transporters, and suggest how verapamil inhibits MATE-mediated multidrug efflux. Our findings offer insights into how MATE transporters extrude chemically and structurally dissimilar drugs and could inform the design of new strategies for tackling multidrug resistance.

  20. Inventory of nutrients in the Bohai

    NASA Astrophysics Data System (ADS)

    Mei Liu, Su; Wei Li, Ling; Zhang, Zhinan

    2011-10-01

    Biogeochemical observations were carried out to address the influence of major sources on nutrient composition and the ecosystem of the Bohai. Relatively high concentrations of nutrients off the Huanghe mouth and the shallow water areas were observed in the Bohai suggesting the effects of tidal and residual currents and anthropogenic perturbation. Sediment in the Bohai represents a source for ammonium, phosphate and dissolved silicate, while it is a sink for nitrite and nitrate. Benthic nutrient fluxes were 2-3 times higher than the riverine input with the regeneration rate of phosphate being slower relative to DIN and dissolved silicate. The release of dissolved silicate and phosphate from sediments may mitigate the decrease of dissolved silicate and phosphate due to the reduction of freshwater discharge. Compared with submarine groundwater discharge, nutrient regeneration in sediment provides similar DIN flux, 2-5 times phosphate and dissolved silicate fluxes. DIN/P molar ratios in the three mentioned sources were 155-845, indicating that phosphorus limitation for phytoplankton growth could be intensified, which likely results in changes of ecosystems of the Bohai.

  1. Filmless Radiographic System For Army Field Hospitals

    NASA Astrophysics Data System (ADS)

    Siedband, Melvin P.; Grenzow, Frank C.; Gray, James; Heilman, Craig A.; Zhang, Hui L.

    1989-05-01

    Small computers incorporating hard disc memory, multiple high resolution monitors and the small computer systems interface (SCSI) can be used for low-cost filmless radiography. A system has been constructed which can perform all of the functions required of a small clinic or field hospital including scheduling, reporting, image acquisition and display, image annotation, image storage and transmission, and control of peripheral devices. The peripheral devices include an optical card reader/writer, an optical disc reader/writer, a SCSI to DIN/PACS port, an Ethernet port and a SCSI to a long distance telephone/computer port, the Integrated Services Digital Network (ISDN) port. Individual patient optical data cards may be prepared, all images and reports may be archived in a small optical disc in the computer, other image sources may be coupled to the system via the DIN/PACS port, data may be exchanged with the local DIN via the Ethernet port and with distant sites via the ISDN port. The small optical data cards, about the size of a credit card, are used for individual patient images and reports. An independent viewer may be used to display the contents of the cards. The result is a complete "filmless and paperless" medical imaging system. The system was developed on Contract DAMD17-88C-8058 with the US Army Medical Research and Development Command.

  2. The effect of water exchange on bacterioplankton depletion and inorganic nutrient dynamics in coral reef cavities

    NASA Astrophysics Data System (ADS)

    van Duyl, F. C.; Scheffers, S. R.; Thomas, F. I. M.; Driscoll, M.

    2006-03-01

    We studied the effect of water exchange on the depletion (or accumulation) of bacterioplankton, dissolved organic matter and inorganic nutrients in small open framework cavities (50-70 l) at 15 m depth on the coral reef along Curaçao, Netherlands Antilles. The bacterioplankton removal rate in cavities increased with increasing water exchange rates up to a threshold of 0.0045 s-1, reaching values of 50-100 mg C m-2 total interior cavity surface area (CSA) per day. Beyond the threshold, bacterioplankton removal dropped. The cryptic community is apparently adapted to the average water exchange in these cavities (0.0041 s-1). Dissolved inorganic nitrogen (DIN), nitrate + nitrite (NO x ) in particular, accumulated in cavity water and the accumulation decreased with increasing water exchange. Net NO x effluxes exceeded net DIN effluxes from cavities (average efflux rate of 1.9 mmol NO x vs. 0.8 mmol DIN m-2 interior CSA per day). The difference is ascribed to net ammonium losses (NH4) in cavities at reef concentrations >0.025 μM NH4, possibly due to enhanced nitrification. Dissolved inorganic phosphate accumulated in cavities, but was not related to water exchange. The cryptic biota in cavities depend on water exchange for optimization of consumption of bacterioplankton and removal of inorganic nitrogen. Coral cavities are an evident sink of bacterioplankton and a source of NO x and PO{4/3-}.

  3. Transformation of irregular shaped silver nanostructures into nanoparticles by under water pulsed laser melting.

    PubMed

    Yadavali, S; Sandireddy, V P; Kalyanaraman, R

    2016-05-13

    The ability to easily manufacture nanostructures with a desirable attribute, such as well-defined size and shape, especially from any given initial shapes or sizes of the material, will be helpful towards accelerating the use of nanomaterials in various applications. In this work we report the transformation of discontinuous irregular nanostructures (DIN) of silver metal by rapid heating under a bulk fluid layer. Ag films were changed into DIN by dewetting in air and subsequently heated by nanosecond laser pulses under water. Our findings show that the DIN first ripens into elongated structures and then breaks up into nanoparticles. From the dependence of this behavior on laser fluence we found that under water irradiation reduced the rate of ripening and also decreased the characteristic break-up length scale of the elongated structures. This latter result was qualitatively interpreted as arising from a Rayleigh-Plateau instability modified to yield significantly smaller length scales than the classical process due to pressure gradients arising from the rapid evaporation of water during laser melting. These results demonstrate that it is possible to fabricate a dense collection of monomodally sized Ag nanoparticles with significantly enhanced plasmonic quality starting from the irregular shaped materials. This can be beneficial towards transforming discontinuous Ag films into nanostructures with useful plasmonic properties, that are relevant for biosensing applications. PMID:27041091

  4. FOREWORD: VI Workshop in Electron Volt Neutron Spectroscopy: Frontiers and Horizons

    NASA Astrophysics Data System (ADS)

    Seel, A. G.; Senesi, R.; Fernandez-Alonso, F.

    2014-12-01

    January 2014 saw the congregation in Abingdon (UK) of scientists from across the world, to discuss the current state and future of spectroscopy using epithermal neutrons. This meeting was the sixth in a series of workshops held in collaboration between the Science and Technology Facilities Council (UK) and the Consiglio Nazionale delle Ricerche (Italy), aimed at bringing together researchers with an interest in the use of electron-volt neutrons in spectroscopic studies [1]. This technique is termed Deep Inelastic Neutron Scattering (DINS), and also Neutron Compton Scattering (NCS) in reference to the analogy with Compton scattering of X-rays from electrons. In particular, this meeting centred jointly around experimentalists and theoreticians, formulating animated discussions as to the current overview of DINS and future horizons facing the field. The use of electron Volt neutrons for spectroscopic measurements dates back to the advent of proton-driven spallation neutron sources in the 1970s and 1980s. Following an initial scientific meeting in Los Alamos (USA) [2], the first two meetings in this series were held in Abingdon (UK) in 1995 and 1998, with subsequent meetings held in Santa Fe (USA) in 2005 [3], Oak Ridge National Laboratory (USA) in 2006 [4], and Rome, Italy; in 2010 [5]. The flagship instrument serving a continual user-programme for DINS measurements, and the main focus of this meeting, has been the VESUVIO spectrometer at ISIS [6, 7]. Subsequent instruments like SEQUOIA in the USA [8, 9] and a newer spectrometer in the Bariloche LINAC in Argentina [10, 11] have also been commissioned and began serving a growing user community. The availability of DINS measurements has extended the range of possible spectroscopic techniques that utilise neutrons into the kinematic region of high energy and momentum transfers, shown schematically in Figure 1. Spectroscopic instrument suites such as that of ISIS are thus able to probe processes on timescales across nine

  5. Unraveling the nitrogen isotopic signature of symbiotic corals

    NASA Astrophysics Data System (ADS)

    Devlin, Q.; Swart, P. K.; Altabet, M. A.

    2013-12-01

    Coral reefs thrive in shallow, tropical, low nutrient waters. Nutrient inputs to a reef environment are often interpreted by measuring the nitrogen isotopic composition of reef organisms. The δ15N signature of scleractinian corals has been historically measured to assess the presence of anthropogenic influences such as sewage and fertilizer runoff. The majority of reef building corals form a symbiotic partnership with the dinoflagellate algae, Symbiodinium microadriaticum. The δ15N signature of symbiotic corals is complex as it is not only dependent on nitrogen acquisition by the coral, but also by the algal symbionts that reside within the gastrodermal tissue layer. The relationship between the δ15N of dissolved inorganic nitrogen (DIN) and the δ15N of coral tissue has not been established. The aim of this study is to identify considerations necessary when interpreting nitrogen sources based on δ15N of coral tissue. Incubations were carried out in order to measure isotopic fractionation associated with nitrate and ammonium incorporation by the Pacific branching coral, Pocillopora damicornis. We investigated the dependence of nitrogen isotope fractionation on species of DIN (nitrate or ammonium), concentration of DIN (range: 1-50 μM N), genetic diversity of algal symbionts (clade C or clade D) and light levels.

  6. Human polymerase kappa uses a template-slippage deletion mechanism, but can realign the slipped strands to favour base substitution mutations over deletions.

    PubMed

    Mukherjee, Purba; Lahiri, Indrajit; Pata, Janice D

    2013-05-01

    Polymerases belonging to the DinB class of the Y-family translesion synthesis DNA polymerases have a preference for accurately and efficiently bypassing damaged guanosines. These DinB polymerases also generate single-base (-1) deletions at high frequencies with most occurring on repetitive 'deletion hotspot' sequences. Human DNA polymerase kappa (hPolκ), the eukaryotic DinB homologue, displays an unusual efficiency for to extend from mispaired primer termini, either by extending directly from the mispair or by primer-template misalignment. This latter property explains how hPolκ creates single-base deletions in non-repetitive sequences, but does not address how deletions occur in repetitive deletion hotspots. Here, we show that hPolκ uses a classical Streisinger template-slippage mechanism to generate -1 deletions in repetitive sequences, as do the bacterial and archaeal homologues. After the first nucleotide is added by template slippage, however, hPolκ can efficiently realign the primer-template duplex before continuing DNA synthesis. Strand realignment results in a base-substitution mutation, minimizing generation of more deleterious frameshift mutations. On non-repetitive sequences, we find that nucleotide misincorporation is slower if the incoming nucleotide can correctly basepair with the nucleotide immediately 5' to the templating base, thereby competing against the mispairing with the templating base. PMID:23558743

  7. POTENTIAL IMPACT OF TANK F FLUSH SOLUTION ON H-CANYON EVAPORATOR OPERATION

    SciTech Connect

    Kyser, E.; Fondeur, F.; Fink, S.

    2010-09-13

    Previous chemical analysis of a sample from the liquid heel found in Tank F of the High Activity Drain (HAD) system in F/H laboratory revealed the presence of n-paraffin, tributyl phosphate (TBP), Modifier from the Modular Caustic-Side Solvent Extraction Unit (MCU) process and a vinyl ester resin that is very similar to the protective lining on Tank F. Subsequent analyses detected the presence of a small amount of diisopropylnaphthalene (DIN) (major component of Ultima Gold{trademark} AB liquid scintillation cocktail). Indications are that both vinyl ester resin and DIN are present in small amounts in the flush solution. The flush solution currently in the LR-56S trailer likely has an emulsion which is believed to contain a mixture of the reported organic species dominated by TBP. An acid treatment similar to that proposed to clear the HAD tank heel in F/H laboratory was found to allow separation of an organic phase from the cloudy sample tested by SRNL. Mixing of that clear sample did re-introduce some cloudiness that did not immediately clear but that cloudiness is attributed to the DIN in the matrix. An organic phase does quickly separate from the cloudy matrix allowing separation by a box decanter in H-Canyon prior to transfer to the evaporator feed tank. This separation should proceed normally as long as the emulsion is broken-up by acidification.

  8. Capturing the fingerprint of Etna volcano activity in gravity and satellite radar data

    PubMed Central

    Negro, Ciro Del; Currenti, Gilda; Solaro, Giuseppe; Greco, Filippo; Pepe, Antonio; Napoli, Rosalba; Pepe, Susi; Casu, Francesco; Sansosti, Eugenio

    2013-01-01

    Long-term and high temporal resolution gravity and deformation data move us toward a better understanding of the behavior of Mt Etna during the June 1995 – December 2011 period in which the volcano exhibited magma charging phases, flank eruptions and summit crater activity. Monthly repeated gravity measurements were coupled with deformation time series using the Differential Synthetic Aperture Radar Interferometry (DInSAR) technique on two sequences of interferograms from ERS/ENVISAT and COSMO-SkyMed satellites. Combining spatiotemporal gravity and DInSAR observations provides the signature of three underlying processes at Etna: (i) magma accumulation in intermediate storage zones, (ii) magmatic intrusions at shallow depth in the South Rift area, and (iii) the seaward sliding of the volcano's eastern flank. Here we demonstrate the strength of the complementary gravity and DInSAR analysis in discerning among different processes and, thus, in detecting deep magma uprising in months to years before the onset of a new Etna eruption. PMID:24169569

  9. DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli

    SciTech Connect

    Kenyon, C.J.; Walker, G.C.

    1988-05-01

    Operon fusions in Escherichia coli were obtained that showed increased beta-galactosidase expression in response to treatment with the DNA-damaging agent mitomycin C. These fusions were generated by using the Mud(ApR, lac) vector to insert the lactose structural genes randomly into the bacterial chromosome. Induction of beta-galactosidase in these strains, which carried fusions of lac to these din (damage-inducible) loci, was (i) triggered by UV light as well as by mitomycin C and (ii) abolished by either a recA- or a lexA- mutation. Similar characteristics of induction were observed when the lactose genes were fused to a prophage lambda promoter by using Mud(ApR, lac). These results indicate that E. coli contains a set of genes that, like prophage lambda genes, are expressed in response to DNA-damaging agents and regulated by the recA and lexA gene products. These din genes map at five bacterial loci. One din::Mud(ApR, lac) insertion results in a UV-sensitive phenotype and may be within the uvrA transcriptional unit.

  10. Complementary Use of Information from Space-Based Dinsar and Field Measuring Systems for Operational Monitoring Purposes in Open Pit Iron Mines of Carajas Mining Complex (brazilian Amazon Region)

    NASA Astrophysics Data System (ADS)

    Paradella, W. R.; Mura, J. C.; Gama, F. F.; Santos, A. R.; Silva, G. G.; Galo, M.; Camargo, P. O.; Silva, A. Q.

    2015-04-01

    Now spanning five simultaneous open-pit operations with exploration carried out through open pit benching, Carajas complex encompasses the world's largest iron reserves. Open pit mining operations in the area can lead to slope instabilities with risks to personnel, equipment and production due to intense excavations in rock products of low geomechanical quality, blasting practices and heavy precipitation. Thus, an effective prediction and management of surface deformations should be a key concern for the mining operations. The ground displacement monitoring techniques in Carajas include surface measurement techniques at discrete points (total station/reflective prisms) and over area using SSR (Slope Stability Radar, a ground based radar). On the other hand, DInSAR techniques are receiving relevance in the mining industry for reasons such a synoptic and continuous coverage without the need for ground instrumentation and a point-to-point good accuracy of measuring displacements (millimeter to centimeter scale) over a dense grid. Using a stack of 33 StripMap TerraSAR-X images acquired over Carajas covering the time span from March 2012 to April 2013, a monitoring approach is discussed based on the complementary use of information provided by DInSAR (DInSAR Time-Series and Persistent Scatterer Interferometry) and surface measuring techniques (total station/prisms, ground-based radar).

  11. Structural basis for the blockade of MATE multidrug efflux pumps

    PubMed Central

    Radchenko, Martha; Symersky, Jindrich; Nie, Rongxin; Lu, Min

    2015-01-01

    Multidrug and toxic compound extrusion (MATE) transporters underpin multidrug resistance by using the H+ or Na+ electrochemical gradient to extrude different drugs across cell membranes. MATE transporters can be further parsed into the DinF, NorM and eukaryotic subfamilies based on their amino-acid sequence similarity. Here we report the 3.0 Å resolution X-ray structures of a protonation-mimetic mutant of an H+-coupled DinF transporter, as well as of an H+-coupled DinF and a Na+-coupled NorM transporters in complexes with verapamil, a small-molecule pharmaceutical that inhibits MATE-mediated multidrug extrusion. Combining structure-inspired mutational and functional studies, we confirm the biological relevance of our crystal structures, reveal the mechanistic differences among MATE transporters, and suggest how verapamil inhibits MATE-mediated multidrug efflux. Our findings offer insights into how MATE transporters extrude chemically and structurally dissimilar drugs and could inform the design of new strategies for tackling multidrug resistance. PMID:26246409

  12. Multislice simulation of transmission electron microscopy imaging of helium bubbles in Fe

    SciTech Connect

    Yao, Bo; Edwards, Danny J.; Kurtz, Richard J.; Odette, George R.; Yamamoto, Takuya

    2012-10-04

    Formation of nanoscale helium (He) bubbles in reduced activation ferritic/martensitic steels may lead to degradation of mechanical properties of materials. Transmission electron microscopy (TEM) has been commonly used to image the Fresnel contrast of He bubbles using a defocus of 0.5 µm ~ 1 µm. This paper presents our study of multislice simulation of the size correlation between imaged Fresnel rings and the actual He bubbles. It was found that for bubbles equal to or larger than 3 nm in diameter, the imaged bubble size, represented by its inner diameter of the first dark Fresnel ring (Din) in under-focused imaging conditions, increases with increasing electron-beam incoherency, but decreases with increasing defocus. The electron-beam accelerating voltage, bubble size, bubble position, and TEM sample thickness were found to have no significant influence on the deviation of Din from the actual bubble size (D0). For bubbles equal to or smaller than 2 nm, however, Din/Do increases dramatically with increasing defocus when it is above a threshold defocus. It was also suggested by this study that He bubbles can be differentiated from argon (Ar) bubbles by contrast differences.

  13. Transformation of irregular shaped silver nanostructures into nanoparticles by under water pulsed laser melting

    NASA Astrophysics Data System (ADS)

    Yadavali, S.; Sandireddy, V. P.; Kalyanaraman, R.

    2016-05-01

    The ability to easily manufacture nanostructures with a desirable attribute, such as well-defined size and shape, especially from any given initial shapes or sizes of the material, will be helpful towards accelerating the use of nanomaterials in various applications. In this work we report the transformation of discontinuous irregular nanostructures (DIN) of silver metal by rapid heating under a bulk fluid layer. Ag films were changed into DIN by dewetting in air and subsequently heated by nanosecond laser pulses under water. Our findings show that the DIN first ripens into elongated structures and then breaks up into nanoparticles. From the dependence of this behavior on laser fluence we found that under water irradiation reduced the rate of ripening and also decreased the characteristic break-up length scale of the elongated structures. This latter result was qualitatively interpreted as arising from a Rayleigh–Plateau instability modified to yield significantly smaller length scales than the classical process due to pressure gradients arising from the rapid evaporation of water during laser melting. These results demonstrate that it is possible to fabricate a dense collection of monomodally sized Ag nanoparticles with significantly enhanced plasmonic quality starting from the irregular shaped materials. This can be beneficial towards transforming discontinuous Ag films into nanostructures with useful plasmonic properties, that are relevant for biosensing applications.

  14. Increasing nutrient concentrations and the rise and fall of a coastal fishery; a review of data from the Nile Delta, Egypt

    NASA Astrophysics Data System (ADS)

    Oczkowski, Autumn; Nixon, Scott

    2008-04-01

    There is a conceptual basis, and some empirical evidence, that increasing nutrient loads to coastal waterbodies will initially increase ecosystem productivity up to a threshold, beyond which secondary productivity and fishery yields will decline. Here we have compiled data from the Egyptian and international literature for fish landings and inorganic nutrient (nitrogen and phosphorus) data from four large coastal lagoons (63-500 km 2) on Egypt's Nile Delta to provide evidence for the initially positive, but then negative, response of fishery yields to increased nutrient supply across a very wide range of enrichment (up to 1 mM dissolved organic nitrogen, DIN). Taking the data from the four lagoons as an aggregate, fish landings increase with increased nutrients up to a peak in landings at approximately 100 μM DIN, beyond which there was an exponential decline in landings. It appears that pesticide and heavy metal contamination and overfishing played only minor roles in the lowered fishery yield at highest DIN concentrations. We do not have sufficient evidence about the specific mechanisms that led to the decline of the fishery, but suspect that some feature of eutrophication—low oxygen, for example, may have been involved.

  15. The nitrogen cycle in the aftermath of the mid-Cryogenian snowball Earth glaciation.

    NASA Astrophysics Data System (ADS)

    Junium, C. K.; Hurtgen, M.; Porter, S.; Halverson, G. P.; Riedman, L. A.

    2014-12-01

    The geochemical record through the mid-Cryogenian (Sturtian) glaciation suggests an extraordinary resumption of productivity following snowball glaciation. Here we present new nitrogen and carbon isotopic data from five sites in the Centralian Superbasin of Australia, and Tasmania. Our data suggest that post-glacial marine conditions were anoxic and nutrient replete, which helped fuel the biological recovery after snowball glaciation. Nitrogen isotope values increase from +5‰ to +9‰ at the glacial termination and decrease to +2‰ through the carbon isotope increase that characterizes the post-glacial interval. Oxidation of the ocean following glaciation fostered loss of DIN via ammonia oxidation coupled with nitrate reduction in addition to drawdown of DIN by high productivity. We interpret the decrease in δ15N as an increase in the relative importance of nitrogen fixation as compensatory response to DIN loss. The long-term low in δ15N values suggests that nitrogen fixation played an important role in maintaining high fractional burial of organic carbon as indicated by the carbon isotope record. Site-to-site differences in the δ13Corg records suggest a strong role for local carbon cycling, vertical mixing, and are consistent with a large surface-to-deep gradient in the δ13C of DIC. If this interpretation is correct, our results are consistent with high phosphate concentrations and a vigorous biological pump in the post-snowball world.

  16. Land deformation in Saint Louis, Missouri measured by ALOS InSAR and PolINSAR validated with DGPS base stations

    NASA Astrophysics Data System (ADS)

    Ghulam, A.

    2011-12-01

    DInSAR is a solid technique to estimate land subsidence and rebound using phase information from multiple SAR acquisitions over the same location from the same orbits, but from a slightly different observing geometry. However, temporal decorrelation and atmospheric effects are often a challenge to the accuracy of the DInSAR measurements. Such uncertainties may be overcome using time series interferogram stacking, e.g., permanent scatterer interferometry (Ferretti, et al., 2000, 2001). However, it requires large number of image collections. In this paper, interferometric synthetic aperture radar (InSAR) data pairs from the Phased Array type L-band Synthetic Aperture Radar (PALSAR) sensor onboard Advanced Land Observing Satellite (ALOS) are used to measure seasonal and annual land surface deformation over Saint Louis, Missouri. The datasets cover four years of time period spanning from 2006 to 2010. With the limited data coverage that is not suitable for permanent scatterer interferometry, the paper demonstrates the efficacy of dual pair interferometry from both fine-beam single polarization mode and dual-pol polarimetric images and short baseline interferometry (SBAS) approach (Berardino, et al., 2002) with an estimation accuracy comparable to differential global position systems (DGPS). We also present the impact of using assumed phase-stable ground control points versus GPS base stations for orbital refinement and phase unwrapping on overall measurement accuracy by comparing the deformation results from DInSAR and Polarimetric InSAR with DGPS base stations and ground truthing.

  17. Surface displacement studies using differential SAR interferometry: an overview

    NASA Astrophysics Data System (ADS)

    Gupta, Sonal; Sajith V., K.; Arora, Manoj K.; Sharma, Mukut L.

    2006-12-01

    The differential SAR interferometry (DInSAR) has been increasing used to monitor ground surface displacements, which may be caused by various natural disasters such as earthquakes, landslides, mining activities, avalanches etc. Conventionally, these displacements were being estimated through field measurements, which are time consuming, hazardous and with data collected over few point locations. Since all the development and rehabilitation works after a natural disaster strikes is carried out on regional basis, any information at spatial level is advantageous in planning, management and monitoring activities. In recent years, the application of Differential SAR interferometry is gaining momentum to estimate the surface displacements at millimeter level accuracy. The displacement maps produced via this technique provide information at spatial level in the region thereby assisting in judicious developmental and planning works in an efficient and cost-effective manner. The aim of this paper is provide an overview of the use of Differential SAR Interferometry (DinSAR) technology for the study of surface displacements. As a case study, land subsidence occurred due to coal mining in Jharia coal fields, Jharkhand, have been estimated through this technique. All the procedural steps in implementing the approach based on DinSAR have been explained in a simplified manner.

  18. Trends in nutrient concentrations in Latvian rivers and the response to the dramatic change in agriculture

    NASA Astrophysics Data System (ADS)

    Stålnacke, P.; Grimvall, A.; Libiseller, C.; Laznik, M.; Kokorite, I.

    2003-12-01

    In recent years, the use of fertilisers in the Baltic countries (Estonia, Latvia, and Lithuania) has decreased at an unprecedented rate. The import of mineral fertilisers and feed stuff became almost non-existent, and extensive slaughtering of livestock reduced the amount of manure. In Latvia, the purchase of mineral fertilisers decreased by a factor of 15 between 1987 and 1996 and the number of livestock decreased with a factor of almost 4 during the same time period. Such abrupt and comprehensive changes in land use have never before occurred in the history of modern European agriculture. Here, the impact that this dramatic reduction has had on concentrations of nutrients in Latvian rivers is examined. To discern temporal changes, statistical analyses were undertaken on time series of nutrient concentrations and relationships between concentrations and runoff at 12 sampling sites in ten Latvian rivers covering drainage areas from 334 to 64,000 km 2. Considering the study period 1987-1998, only four of the 12 sites showed statistically significant downward trends (one-sided test at the 5% level) in the dissolved inorganic nitrogen (DIN=NO 3-N+NO 2-N+NH 4-N) data. There are probably two main explanations for the weak DIN trends. Firstly, long water-transit times in the soilwater and groundwater may have caused substantial time lag between changes in input and output of nitrate in the studied catchments. Secondly, the loss of DIN might have been dominated by mineralisation of large pools of organic nitrogen that have accumulated over several years. These inferences are supported by (i) a hydrograph recession analysis and (ii) indications of DIN transformation processes, presumably denitrification, in smaller streams and channels, based on measurements in small agricultural catchments (1-4 km 2) in Estonia and Latvia. Formal testing of trends in phosphorus data revealed that marked drops occurred in riverine concentrations at six sites in 1987-1998. A joint analysis

  19. Experimental evidence for an effect of early-diagenetic interaction between labile and refractory marine sedimentary organic matter on nitrogen dynamics

    NASA Astrophysics Data System (ADS)

    Turnewitsch, Robert; Domeyer, Bettina; Graf, Gerhard

    2007-05-01

    In most natural sedimentary systems labile and refractory organic material (OM) occur concomitantly. Little, however, is known on how different kinds of OM interact and how such interactions affect early diagenesis in sediments. In a simple sediment experiment, we investigated how interactions of OM substrates of different degradability affect benthic nitrogen (N) dynamics. Temporal evolution of a set of selected biogeochemical parameters was monitored in sandy sediment over 116 days in three experimental set-ups spiked with labile OM (tissue of Mytilus edulis), refractory OM (mostly aged Zostera marina and macroalgae), and a 1:1 mixture of labile and refractory OM. The initial amounts of particulate organic carbon (POC) were identical in the three set-ups. To check for non-linear interactions between labile and refractory OM, the evolution of the mixture system was compared with the evolution of the simple sum of the labile and refractory systems, divided by two. The sum system is the experimental control where labile and refractory OM are virtually combined but not allowed to interact. During the first 30 days there was evidence for net dissolved-inorganic-nitrogen (DIN) production followed by net DIN consumption. (Here 'DIN' is the sum of ammonium, nitrite and nitrate.) After ˜ 30 days a quasi steady state was reached. Non-linear interactions between the two types of OM were reflected by three main differences between the early-diagenetic evolutions of nitrogen dynamics of the mixture and sum (control) systems: (1) In the mixture system the phases of net DIN production and consumption commenced more rapidly and were more intense. (2) The mixture system was shifted towards a more oxidised state of DIN products [as indicated by increased (nitrite + nitrate)/(ammonium) ratios]. (3) There was some evidence that more OM, POC and particulate nitrogen were preserved in the mixture system. That is, in the mixture system more particulate OM was preserved while a higher

  20. Salinity predicts the distribution of chlorophyll a spring peak in the southern North Sea continental waters

    NASA Astrophysics Data System (ADS)

    Desmit, Xavier; Ruddick, Kevin; Lacroix, Geneviève

    2015-09-01

    In the North Sea, the coastal waters of Belgium and The Netherlands regularly exhibit intense spring phytoplankton blooms where species such as Phaeocystis recurrently form a potential ecological nuisance. In the Belgian and Dutch continental shelves (BCS and DCS), we observe a direct correlation between the chlorophyll a spring maximum (Chlmax) and the nutrients (DIN and DIP) available for the bloom. As the nutrients are themselves strongly correlated with salinity, a rationale is developed to predict Chlmax from winter salinity. The proposed rationale is first tested in a theoretical case with a 3D-biogeochemical model (3D-MIRO&CO). The method is then applied to independent sets of in situ observations over 20 years in the BCS and the DCS, and to continuous FerryBox data in April 2008. Linear regressions explain the relationships between winter nutrients and winter salinity (R2 = 0.88 to 0.97 with model results, and R2 = 0.83 to 0.96 with in situ data). The relationship between Chlmax and the available nutrients across the salinity gradient is also explained by yearly linear regressions (R2 = 0.82 to 0.94 with model results, and R2 = 0.46 to 0.98 with in situ data). Empirical 'DIP requirement' and 'DIN requirement' for the spring biomass bloom formation are derived from the latter relationships. They depend i.a. on the losses from phytoplankton during the spring bloom formation, and therefore show some interannual variability (8-12% for DIP and 13-20% for DIN). The ratio between nutrient requirements allows predicting in winter which nutrient will eventually limit the spring biomass bloom along the salinity gradient. DIP will generally be limiting in the coastal zone, whereas DIN will generally be limiting offshore, the switch occurring typically at salinity 33.5 in the BCS and 33.6 in the DCS. N reduction should be prioritized to limit Phaeocystis in the coastal zone, with target winter DIN:DIP ratios below 34.4 molN molP-1 in the BCS, or 28.6 molN molP- 1 in

  1. Benthic nitrogen cycling traversing the capitalize peruvian oxygen minimum zone

    NASA Astrophysics Data System (ADS)

    Bohlen, L.; Dale, A. W.; Sommer, S.; Mosch, T.; Hensen, C.; Noffke, A.; Scholz, F.; Wallmann, K.

    2011-10-01

    Benthic nitrogen (N) cycling was investigated at six stations along a transect traversing the Peruvian oxygen minimum zone (OMZ) at 11°S. An extensive dataset including porewater concentration profiles and in situ benthic fluxes of nitrate (NO 3-), nitrite (NO 2-) and ammonium (NH 4+) was used to constrain a 1-D reaction-transport model designed to simulate and interpret the measured data at each station. Simulated rates of nitrification, denitrification, anammox and dissimilatory nitrate reduction to ammonium (DNRA) by filamentous large sulfur bacteria (e.g. Beggiatoa and Thioploca) were highly variable throughout the OMZ yet clear trends were discernible. On the shelf and upper slope (80-260 m water depth) where extensive areas of bacterial mats were present, DNRA dominated total N turnover (⩽2.9 mmol N m -2 d -1) and accounted for ⩾65% of NO 3- + NO 2- uptake by the sediments from the bottom water. Nonetheless, these sediments did not represent a major sink for dissolved inorganic nitrogen (DIN = NO 3- + NO 2- + NH 4+) since DNRA reduces NO 3- and, potentially NO 2-, to NH 4+. Consequently, the shelf and upper slope sediments were recycling sites for DIN due to relatively low rates of denitrification and high rates of ammonium release from DNRA and ammonification of organic matter. This finding contrasts with the current opinion that sediments underlying OMZs are a strong sink for DIN. Only at greater water depths (300-1000 m) did the sediments become a net sink for DIN. Here, denitrification was the major process (⩽2 mmol N m -2 d -1) and removed 55-73% of NO 3- and NO 2- taken up by the sediments, with DNRA and anammox accounting for the remaining fraction. Anammox was of minor importance on the shelf and upper slope yet contributed up to 62% to total N 2 production at the 1000 m station. The results indicate that the partitioning of oxidized N (NO 3-, NO 2-) into DNRA or denitrification is a key factor determining the role of marine sediments as DIN

  2. Growth of a young pingo in the Canadian Arctic observed by RADARSAT-2 interferometric satellite radar

    NASA Astrophysics Data System (ADS)

    Samsonov, S. V.; Lantz, T. C.; Kokelj, S. V.; Zhang, Y.

    2015-11-01

    Advancements in radar technology are increasing our ability to detect earth surface deformation in permafrost environments. In this paper we use satellite Differential Interferometric Synthetic Aperture Radar (DInSAR) to describe the growth of a previously unreported pingo in the Tuktoyaktuk Coastlands. High-resolution RADARSAT-2 imagery (2011-2014) analyzed with the Multidimensional Small Baseline Subset (MSBAS) DInSAR revealed a maximum 2.7 cm yr-1 of domed uplift located in a drained lake basin. Observed changes in elevation were modeled as a 348 m × 290 m uniformly loaded elliptical plate with clamped edge. Model results suggest that this feature is one of the largest diameter pingos in the region that is presently growing. Analysis of historical aerial photographs showed that ground uplift at this location initiated sometime between 1935 and 1951 following lake drainage. Uplift is largely due to the growth of intrusive ice, because the 9 % expansion of pore water associated with permafrost aggradation into saturated sands is not sufficient to explain the observed short- and long-term deformation rates. The modeled thickness of permafrost using the Northern Ecosystem Soil Temperature (NEST) was consistent with the maximum height of this feature and the 1972-2014 elevation changes estimated from aerial photographs, suggesting that permafrost aggradation is resulting in the freezing a sub-pingo water lens. Seasonal variation in the uplift rate seen in the DInSAR data also matches the modeled seasonal pattern in the deepening rate of freezing front. This study demonstrates that interferometric satellite radar can successfully contribute to understanding the dynamics of terrain uplift in response to permafrost aggradation and ground ice development in remote polar environments, and highlights possible application of detecting deformation of Martian landscapes. However, our DInSAR data did not show clear growth at other smaller pingos in contrast with field studies

  3. Linking Land-Use to Submarine Groundwater Discharge Nutrient Fluxes on Maui, Hawaii

    NASA Astrophysics Data System (ADS)

    Bishop, J. M.; Glenn, C. R.; Amato, D. W.; Dulaiova, H.

    2014-12-01

    Fertilized agricultural lands, wastewater injection, and areas with high septic system density each have substantial potential for contributing excess nutrients to the coastal waters of islands via submarine groundwater discharge (SGD). We evaluated the coastal impacts of such land-use around the island of Maui using stable isotopes (δ15N of NO3-; δ18O and δ2H of H2O) and nutrient concentrations from wells, springs, beach seeps, SGD, and coastal waters, and coupled these to coastal water SGD and nutrient fluxes using 222Rn mass balance. Flowpaths and recharge elevations for groundwater samples were determined using the δ18OH2O of samples, recharge data, modeled groundwater head, and published local meteoric water lines. Coastal groundwater samples whose flowpaths transect sugarcane plots showed highest dissolved inorganic nitrogen (DIN - NO3- + NO2-) concentrations, ranging from 225 - 450 µM, and the δ15N of those samples was on average 3.3 ± 0.6 ‰ (n = 15), as expected for urea fertilizers applied to commercial sugarcane. Samples whose flowpaths transect large amounts of septic sources showed moderate DIN concentrations (max. 100 µM), but had higher δ15N values (9.7 ± 4.8 ‰, n = 5), reflecting their sewage influence. Due to nitrate reduction during subterranean transit, groundwater and coastal waters proximal to deep, near-coast wastewater injection (Kahului) display the highest average δ15N values encountered (δ15NNO3- 18.8 ± 11.1 ‰, n = 19) and moderate DIN concentrations (max 90 µM). Average SGD nutrient fluxes among the different field sites ranged from 700 - 1,500,000 µmol/d/m of shoreline for DIN and 600 - 22,000 µmol/d/m for orthophosphate. These results indicate that areas with the highest N and P fluxes are areas where coastal groundwater flowpaths transect large tracts of sugarcane production. Although highest SGD rates occur near the Kahului wastewater injection wells (~ 2800 m3/day) the relatively low nutrient concentrations in

  4. Measuring ecosystem nitrogen status: a comparison of proxies

    NASA Astrophysics Data System (ADS)

    Almaraz, M.; Porder, S.

    2013-12-01

    Nitrogen (N) limitation to primary production is widespread in both terrestrial and aquatic systems, and variation in N availability drives differences in ecosystem properties across space and time. Instantaneous measurements of N availability in soil are common, but it is difficult to assess whether proxies commonly used to infer long-term N status over space or time adequately integrate these short-term measurements. Nevertheless, these longer temporal, and larger spatial, scales are relevant to many ecosystem properties and responses to global change. Here we assess how well several proxies that purportedly reflect time or space-integrated N availability correlate with each other when they are measured in the same place. We surveyed the literature and contacted individual investigators to gather data from intact forest and grassland sites where several proxies of long-term N availability had been measured. We focused on the most commonly-used proxies for N status: foliar and soil δ15N, net N mineralization, net nitrification, the ratio of dissolved inorganic to organic N forms in soil solution below the rooting zone (DIN:DON), and the DIN:DON in streams. We found that soil δ15N is positively correlated with foliar δ15N (r=0.63, p=0.0008, n=25), but is negatively correlated with net N mineralization (r=0.73, p=0.0101, n=11). There was not a significant correlation between soil δ15N and nitrification (r=0.08, p=0.8107, n=11), soil solution DIN:DON (r=0.28, p=0.1217, n=32), or stream DIN:DON (r=0.04, p=0.9104, n=9). Foliar δ15N is positively correlated with nitrification (r=0.70, p=0.0359, n=9), but there was not a significant correlation between foliar δ15N and net N mineralization (r=0.63, p=0.0666, n=9), or soil solution DIN:DON (r=0.10, p=0.6290, n=24). We found no significant correlation between net N mineralization and net nitrification (r=0.33, p=0.3232, n=11), nor do we see a significant correlation between soil solution and stream DIN:DON (r=0.58, p

  5. Landscape controls on dissolved nutrients, organic matter and major ions in a suburbanizing watershed

    NASA Astrophysics Data System (ADS)

    Daley, M. L.; McDowell, W. H.

    2010-12-01

    Understanding the relative importance of anthropogenic and natural landscape features that drive spatial variability in water quality is a central challenge in studying the biogeochemistry of heterogeneous landscapes. We quantified the average annual flux and concentration of dissolved inorganic nitrogen (DIN), dissolved organic nitrogen (DON), dissolved organic carbon (DOC), phosphate-P (PO4-P), sodium (Na+) and chloride (Cl-) at ~40 stream sites in three major (51 to 903 km2) NH basins. We used GIS to quantify anthropogenic (e.g. human population density, % impervious surface cover and % agriculture) and natural (e.g. % forest, % wetlands and soil C:N) landscape features for each sub-basin and then employed multiple-regression analysis to relate water quality parameters to landscape characteristics. Anthropogenic features were strong predictors of DIN flux and Na+ and Cl- concentrations, whereas wetland cover (a natural feature) was a significant, but weak predictor of DOC (r2=0.26, p<0.01) and DON (r2 = 0.14, p<0.05) flux. Anthropogenic features could not explain a significant amount of variance in DON or DOC flux. Mean PO4-P concentrations were surprisingly low (<0.015 mg P/L) when compared to the larger range in mean DIN concentrations (0.03 to 0.96 mg/L) and consequently no landscape characteristics could explain a significant amount of spatial variability in PO4-P flux or concentration. Human population density was the single best predictor of DIN flux (r2=0.76, p<0.01), and together with % impervious surface and % agriculture explained 86% (p<0.01) of the total variance. Among all sites, % road pavement was a strong predictor of stream Na+ and Cl- concentrations (r2 = 0.75 to 0.78, p<0.01) and % impervious surface was a stronger predictor (r2 = 0.86 to 0.92, p<0.01) among a subset of sites. Our results suggest that DIN and DON result from different sources in the landscape and although sources of DON and DOC are similar, DON and DOC concentrations respond

  6. Dynamic seasonal nitrogen cycling in response to anthropogenic N-loading in a tropical catchment, Athi-Galana-Sabaki River, Kenya

    NASA Astrophysics Data System (ADS)

    Marwick, T. R.; Tamooh, F.; Ogwoka, B.; Teodoru, C.; Borges, A. V.; Darchambeau, F.; Bouillon, S.

    2013-05-01

    As part of a broader study on the riverine biogeochemistry in the Athi-Galana-Sabaki (A-G-S) River catchment (Kenya), we present data constraining the sources, transit and transformation of multiple nitrogen (N) species as they flow through the A-G-S catchment (~47 000 km2). The data-set was obtained in August-September 2011, November 2011, and April-May 2012, covering the dry season, short-rain season and long-rain season respectively. Release of, largely untreated, waste water from the city of Nairobi had a profound impact on the biogeochemistry of the upper Athi river, leading to low dissolved oxygen (DO) saturation levels (67-36%), high ammonium (NH4+) concentrations (1193-123 μmol L-1), and high dissolved methane (CH4) concentrations (6729-3765 nmol L-1). Total dissolved inorganic nitrogen (DIN) concentrations entering the study area were highest during the dry season (1195 μmol L-1), while total DIN concentration was an order of magnitude lower during the short and long rain seasons (212 and 193 μmol L-1, respectively). During the rain seasons, low water residence time led to relatively minimal instream N-cycling prior to discharge to the ocean. Conversely, increased residence time during the dry season creates two differences comparative to wet season conditions, where (1) intense cycling and removal of DIN in the upper- to mid-catchment leads to significantly less DIN export during the dry season, and (2) as a result of the intense DIN cycling, dry season particulate N export is significantly enriched in the N stable isotope ratio (δ15NPN), strongly reflecting the dominance of organic matter as the prevailing source of riverine nitrogen. The rapid removal of NH4+ in the upper study area during the dry season was accompanied by a quantitatively similar production of NO3- and nitrous oxide (N2O) downstream, pointing towards strong nitrification over this reach during the dry season. Nitrous oxide produced was rapidly degassed downstream, while the

  7. Fate of Upstream Anthropogenic Nitrogen Inputs in a Tropical Catchment, Athi-Galana-Sabaki River, Kenya.

    NASA Astrophysics Data System (ADS)

    Marwick, Trent R.

    2013-04-01

    As part of a broader study on the riverine biogeochemistry in the Athi-Galana-Sabaki (A-G-S) River basin (Kenya), we present data collected during three climatic seasons to constrain the sources, transformations and transit of multiple N species as they flow through the A-G-S basin (~47,000 km2), stretching from downstream of heavily polluted Nairobi and surrounds to the outlet at the Indian Ocean. Total dissolved inorganic nitrogen (DIN) concentrations entering the study area were highest during the dry season (1195 µmol/L), almost completely in the form of ammonium (99.8%), whilst total DIN was an order of magnitude lower during the short and long rain seasons (212 and 193 µmol/L, respectively). Nitrate was the dominant form of DIN entering the study area during the short and long rain seasons (97.9% and 85.6% of total DIN respectively), with the increased flow conditions resulting in minimal instream N-cycling prior to discharge to the ocean. Conversely, longer water residence time and intense cycling and removal of N in the upper- to mid-catchment during the dry season creates two polarities comparative to wet season conditions, where (1) significantly less DIN is exported to the ocean during the dry season, and (2) dry season particulate N export is significantly enriched in δ15N, strongly reflecting the dominance of organic wastes as the source of riverine nitrogen. The rapid removal of ammonium in the upper study area during the dry season was followed by a quantitatively similar production of nitrate and nitrous oxide downstream, pointing towards strong nitrification over this reach during the dry season. The nitrous oxide was rapidly degassed downstream, while the elevated nitrate concentrations steadily decreased to levels observed elsewhere in African river networks. Low pelagic primary production rates over the same reach suggest benthic denitrification was the dominant process controlling the removal of these nitrates, although large cyanobacterial

  8. The generation of antiphase oscillations and synchrony by a rebound-based vertebrate central pattern generator.

    PubMed

    Li, Wen-Chang; Merrison-Hort, Robert; Zhang, Hong-Yan; Borisyuk, Roman

    2014-04-23

    Many neural circuits are capable of generating multiple stereotyped outputs after different sensory inputs or neuromodulation. We have previously identified the central pattern generator (CPG) for Xenopus tadpole swimming that involves antiphase oscillations of activity between the left and right sides. Here we analyze the cellular basis for spontaneous left-right motor synchrony characterized by simultaneous bursting on both sides at twice the swimming frequency. Spontaneous synchrony bouts are rare in most tadpoles, and they instantly emerge from and switch back to swimming, most frequently within the first second after skin stimulation. Analyses show that only neurons that are active during swimming fire action potentials in synchrony, suggesting both output patterns derive from the same neural circuit. The firing of excitatory descending interneurons (dINs) leads that of other types of neurons in synchrony as it does in swimming. During synchrony, the time window between phasic excitation and inhibition is 7.9 ± 1 ms, shorter than that in swimming (41 ± 2.3 ms). The occasional, extra midcycle firing of dINs during swimming may initiate synchrony, and mismatches of timing in the left and right activity can switch synchrony back to swimming. Computer modeling supports these findings by showing that the same neural network, in which reciprocal inhibition mediates rebound firing, can generate both swimming and synchrony without circuit reconfiguration. Modeling also shows that lengthening the time window between phasic excitation and inhibition by increasing dIN synaptic/conduction delay can improve the stability of synchrony. PMID:24760866

  9. Centimeter Cosmo-Skymed Range Measurements for Monitoring Ground Displacements

    NASA Astrophysics Data System (ADS)

    Fratarcangeli, F.; Nascetti, A.; Capaldo, P.; Mazzoni, A.; Crespi, M.

    2016-06-01

    The SAR (Synthetic Aperture Radar) imagery are widely used in order to monitor displacements impacting the Earth surface and infrastructures. The main remote sensing technique to extract sub-centimeter information from SAR imagery is the Differential SAR Interferometry (DInSAR), based on the phase information only. However, it is well known that DInSAR technique may suffer for lack of coherence among the considered stack of images. New Earth observation SAR satellite sensors, as COSMO-SkyMed, TerraSAR-X, and the coming PAZ, can acquire imagery with high amplitude resolutions too, up to few decimeters. Thanks to this feature, and to the on board dual frequency GPS receivers, allowing orbits determination with an accuracy at few centimetres level, the it was proven by different groups that TerraSAR-X imagery offer the capability to achieve, in a global reference frame, 3D positioning accuracies in the decimeter range and even better just exploiting the slant-range measurements coming from the amplitude information, provided proper corrections of all the involved geophysical phenomena are carefully applied. The core of this work is to test this methodology on COSMO-SkyMed data acquired over the Corvara area (Bolzano - Northern Italy), where, currently, a landslide with relevant yearly displacements, up to decimeters, is monitored, using GPS survey and DInSAR technique. The leading idea is to measure the distance between the satellite and a well identifiable natural or artificial Persistent Scatterer (PS), taking in account the signal propagation delays through the troposphere and ionosphere and filtering out the known geophysical effects that induce periodic and secular ground displacements. The preliminary results here presented and discussed indicate that COSMO-SkyMed Himage imagery appear able to guarantee a displacements monitoring with an accuracy of few centimetres using only the amplitude data, provided few (at least one) stable PS's are available around the

  10. Acid rain mitigation experiment shifts a forested watershed from a net sink to a net source of nitrogen.

    PubMed

    Rosi-Marshall, Emma J; Bernhardt, Emily S; Buso, Donald C; Driscoll, Charles T; Likens, Gene E

    2016-07-01

    Decades of acid rain have acidified forest soils and freshwaters throughout montane forests of the northeastern United States; the resulting loss of soil base cations is hypothesized to be responsible for limiting rates of forest growth throughout the region. In 1999, an experiment was conducted that reversed the long-term trend of soil base cation depletion and tested the hypothesis that calcium limits forest growth in acidified soils. Researchers added 1,189 kg Ca(2+) ha(-1) as the pelletized mineral wollastonite (CaSiO3) to a 12-ha forested watershed within the Hubbard Brook Experimental Forest in the White Mountains of New Hampshire. Significant increases in the pH and acid-neutralizing capacity of soils and streamwater resulted, and the predicted increase in forest growth occurred. An unanticipated consequence of this acidification mitigation experiment began to emerge a decade later, with marked increases in dissolved inorganic nitrogen (DIN) exports in streamwater from the treated watershed. By 2013, 30-times greater DIN was exported from this base-treated watershed than from adjacent reference watersheds, and DIN exports resulting from this experiment match or exceed earlier reports of inorganic N losses after severe ice-storm damage within the study watershed. The discovery that CaSiO3 enrichment can convert a watershed from a sink to a source of N suggests that numerous potential mechanisms drive watershed N dynamics and provides new insights into the influence of acid deposition mitigation strategies for both carbon cycling and watershed N export. PMID:27335456

  11. [The highly gifted persons study by Adele Juda 1927-1955. Pinnacle and end of psychiatric genius research in Germany].

    PubMed

    Wiedemann, Ute; Burgmair, Wolfgang; Weber, Matthias M

    2007-01-01

    Between 1927 and 1944 the psychiatrist Adele Juda (*1888-+1949) studied the biographies of more than 600 German-speaking "geniuses" and their families from a period between 1648 and 1920. The concept of this so-called "Höchstbegabtenstudie" (study on high-gifted persons) had been developed by the psychiatrist, human geneticist and racial hygienist Ernst Rüdin, director of the Deutsche Forschungsanstalt für Psychiatrie in Munich from 1917 to 1945. Juda's study was aimed at a re-examination of the "Genie-Irrsins-Hypothese" (genius-madness-theory) having been much discussed in medicine and anthropology since Cesare Lombroso, as it was hardly consistent with some of Rüdin's racial-hygienic concepts. While trying to make a selection of probands as objective as possible and to overcome a so far common purely casuistic approach, Juda's study also gave cause for criticism, for example as to the subjectivity of psychopathological assessment or the political and ideological conditions under which data were gathered. Nevertheless the "Höchstbegabtenstudie" has to be seen as the most extensive and as well as the last scientific piece of research concerning the "Genialenproblem" having been done in the 20th century, with the material gathered being an important cultural-historical source independent of its originally intended use. As one of the most important results Juda was able to prove a significant relation between mental illness and gift. For different reasons this result was not published until after Juda's death by Bruno Schulz, one of her former colleagues at the genealogical-demographic department, in 1953 and 1955. Last not least due to the fact that they came from Rüdin's former institute these publications were not taken much notice of. PMID:17564157

  12. Materials Selection Criteria for Nuclear Power Applications: A Decision Algorithm

    NASA Astrophysics Data System (ADS)

    Rodríguez-Prieto, Álvaro; Camacho, Ana María; Sebastián, Miguel Ángel

    2016-02-01

    An innovative methodology based on stringency levels is proposed in this paper and improves the current selection method for structural materials used in demanding industrial applications. This paper describes a new approach for quantifying the stringency of materials requirements based on a novel deterministic algorithm to prevent potential failures. We have applied the new methodology to different standardized specifications used in pressure vessels design, such as SA-533 Grade B Cl.1, SA-508 Cl.3 (issued by the American Society of Mechanical Engineers), DIN 20MnMoNi55 (issued by the German Institute of Standardization) and 16MND5 (issued by the French Nuclear Commission) specifications and determine the influence of design code selection. This study is based on key scientific publications on the influence of chemical composition on the mechanical behavior of materials, which were not considered when the technological requirements were established in the aforementioned specifications. For this purpose, a new method to quantify the efficacy of each standard has been developed using a deterministic algorithm. The process of assigning relative weights was performed by consulting a panel of experts in materials selection for reactor pressure vessels to provide a more objective methodology; thus, the resulting mathematical calculations for quantitative analysis are greatly simplified. The final results show that steel DIN 20MnMoNi55 is the best material option. Additionally, more recently developed materials such as DIN 20MnMoNi55, 16MND5 and SA-508 Cl.3 exhibit mechanical requirements more stringent than SA-533 Grade B Cl.1. The methodology presented in this paper can be used as a decision tool in selection of materials for a wide range of applications.

  13. Temporal changes and spatial variation of soil oxygen consumption, nitrification and denitrification rates in a tidal salt marsh of the Lagoon of Venice, Italy

    NASA Astrophysics Data System (ADS)

    Eriksson, P. G.; Svensson, J. M.; Carrer, G. M.

    2003-12-01

    The aim of the present study was to investigate seasonal and spatial patterns of soil oxygen consumption, nitrification, denitrification and fluxes of dissolved inorganic nitrogen (DIN) in a tidal salt marsh of the Lagoon of Venice, Italy. In the salt marsh, intact soil cores including overlying water were collected monthly at high tide from April to October in salt marsh creeks and in areas covered by the dominant vegetation, Limonium serotinum. In May, cores were also collected in areas with vegetation dominated by Juncus maritimus and Halimione portulacoides. In laboratory incubations at in situ temperature in the dark, flux rates of oxygen and DIN were monitored in the overlying water of the intact cores. 15N-nitrate was added to the overlying water and nitrification and denitrification were measured using isotope-dilution and -pairing techniques. The results show that highest soil oxygen consumption coincided with the highest water temperature in June and July. The highest denitrification rates were recorded in spring and autumn coinciding with the highest nitrate concentrations. Soil oxygen consumption and nitrification rates differed between sampling sites, but denitrification rates were similar among the different vegetation types. The highest rates were recorded in areas covered with L. serotinum. Burrowing soil macrofauna enhanced oxygen consumption, nitrification and denitrification in April and May. The data presented in this study indicate high temporal as well as spatial variations in the flux of oxygen and DIN, and nitrogen transformations in the tidal salt marshes of the Venice lagoon during the growth season. The results identify the salt marshes of the Venice lagoon as being metabolically very active ecosystems with a high capacity to process nitrogen.

  14. Relationship between two Solomon Islands Earthquakes in 2007 (M8.1), 2010 (M7.1), and Seismic Gap along the Subduction Zone, Revealed by ALOS/PALSAR

    NASA Astrophysics Data System (ADS)

    Miyagi, Y.; Ozawa, T.

    2010-12-01

    The Solomon Islands are located in the southwest of the Pacific Ocean. The Australian, Woodlark, and Solomon Sea plates subduct toward the northeast beneath the Pacific plate. Interaction among these four plates cause complicated tectonics around the Solomon Islands, and have caused interplate earthquakes in the subduction zone (e.g. Lay and Kanamori, 1980; Xu and Schwarts, 1993). On April 1, 2007 (UTC), an M8.1 interplate earthquake occurred in the subduction zone between the Pacific Plate and the Australian Plate. This earthquake was accompanied by a large tsunami and caused considerable damage in the area. The Japan Aerospace Exploration Agency (JAXA) carried out emergency observations using the Phased Array type L-band Synthetic Aperture Rader (PALSAR) installed on Advanced Land Observing Satellite (ALOS), and detected more than 2m of maximum displacement using differential interferometric SAR (DInSAR) technique. Miyagi et al. (2009) estimated a slip distribution of the seismic fault mainly from the PALSAR/DInSAR data and suggested that most of a seismic gap was filled by the 2007 events, but a small seismic gap connecting to an Mw7.0-sized earthquake still remained. On January 3, 2010, an M7.1 earthquake occurred in the vicinity of the remnant seismic gap. ALOS/PALSAR observed epicentral area both before and after the event, and detected crustal deformation associated with the earthquake. We inferred fault model using the PALSAR/DInSAR data and concluded that the 2010 event was the supposed thrust earthquake filling the remnant seismic gap. A distribution of coulomb failure stress change in the epicentral area after the 2007 event suggested the possibility that the 2010 event was triggered by the 2007 earthquake.

  15. Atmospheric organic and inorganic nitrogen inputs to coastal urban and montane Atlantic Forest sites in southeastern Brazil

    NASA Astrophysics Data System (ADS)

    de Souza, Patricia A.; Ponette-González, Alexandra G.; de Mello, William Z.; Weathers, Kathleen C.; Santos, Isimar A.

    2015-06-01

    Tropical regions are currently experiencing changes in the quantity and form of nitrogen (N) deposition as a result of urban and industrial emissions. We quantified atmospheric N inputs to two coastal urban and two montane (400 m and 1000 m) Atlantic Forest sites downwind of the Metropolitan Region of Rio de Janeiro (MRRJ), Brazil, from August 2008 to August 2009. Concentrations of total dissolved nitrogen (TDN), dissolved inorganic nitrogen (DIN) and urea were measured in bulk precipitation at all sites, as well as in canopy throughfall in the lower montane forest. Dissolved organic nitrogen (DON) was calculated as the difference between TDN and DIN (NH4+ + NO3- + NO2-). Annual volume-weighted mean bulk concentrations of all N species were higher at the coastal urban than montane forest sites, with DON accounting for 32-56% and 26-32%, respectively, of the TDN concentration in bulk precipitation. Bulk deposition of TDN ranged 12.1-17.2 kg N ha- 1 yr- 1 and tended to decrease with increasing distance from the coastal urban region. In the lower montane forest, throughfall TDN flux, 34.3 kg N ha- 1 yr- 1, was over 2-fold higher than bulk TDN deposition, and DON comprised 57% of the total N deposited by throughfall to the forest soil. Urea comprised 27% of DON in throughfall compared to up to 100% in bulk precipitation. Our findings show that DON is an important, yet understudied, component of TDN deposition in tropical forest regions, comprising one-third to greater than one-half of the N deposited in rainfall and throughfall. Further, in this lower montane Atlantic Forest site, throughfall DIN flux was 1.5-3 fold higher than the suggested empirical critical load for humid tropical forests, highlighting the potential for increasing N pollution emitted from the MRRJ to impact N cycling in adjacent ecosystems.

  16. Early transcriptomic response of Arabidopsis thaliana to polymetallic contamination: implications for the identification of potential biomarkers of metal exposure.

    PubMed

    Gómez-Sagasti, María T; Barrutia, Oihana; Ribas, Griselda; Garbisu, Carlos; Becerril, José M

    2016-05-01

    Heavy metal contaminated sites are frequently characterized by the simultaneous presence of several heavy metals. However, many studies report metal-induced plant responses after long-term exposure to just one metal. By contrast, whole genome expression microarrays were employed here to investigate the early (3 h) transcriptional responses of Arabidopsis thaliana plants exposed to polymetallic treatment (Pb, Hg, Cu, Cd, Co, Ni, Zn, and Mn) at low (L) and high (H) concentrations. After 3 h of exposure to polymetallic treatment, a total of 1315 noticeably (≥2-fold) and significantly (P < 0.05) differentially expressed genes were identified: 656 and 351 upregulated and 314 and 200 downregulated genes in L and H treatments, respectively. Functional analysis revealed that many genes involved in oxidative stress and perception/signalling/regulation systems were activated. Genes encoding proteins involved in hormone regulation (jasmonic acid, abscisic acid, ethylene, and auxins), glucosinolate metabolism and sulphur and nitrogen transport were also modulated. RT-qPCR analysis of four downregulated (AOP2, SAUR16, BBX31, and MTPC3) and upregulated genes (ASN1, DIN2, BT2, and EXL5), markedly responsive to both L and H treatments, validated our microarray data and suggested the potential of some of these genes (AOP2, SAUR16, ASN1, and DIN2) as early biomarkers of metal exposure. Relevant changes in gene expression occur as early as 3 h after exposure to polymetallic treatment. Four genes deserve further studies as novel putative biomarkers of early metal exposure and also owing to their potential implications in stress-related mechanisms: sulphur balance (AOP2), phytohormone regulation of plant growth and development (SAUR16), ammonium detoxification (ASN1) and senescence (DIN2). PMID:27118254

  17. Factors controlling aquatic dissolved inorganic nitrogen removal and export in suburban watersheds

    NASA Astrophysics Data System (ADS)

    Mineau, M.; Wollheim, W. M.; Stewart, R.; Daley, M.; McDowell, W. H.

    2013-12-01

    Human activity has accelerated the nitrogen (N) cycle and enriched the landscape with N which can result in eutrophication, especially in coastal zones where N is typically limiting. N exported to coastal zones is a function of both N loading to aquatic systems and N removal in transit through the river network. To determine drivers of dissolved inorganic nitrogen (DIN) removal and export from suburban river networks, we compared 2 well-studied suburban New-England watersheds. The Lamprey River watershed (474 km2) in NH has a mean population density of 53 inhabitants per km2 and feeds into the Great Bay estuary which is designated as N impaired. The Ipswich River (400 km2) in MA has a much higher population density with 302 inhabitants per km2 and feeds into the Plum Island estuary, which is not N impaired. Median (2000 - 2009) watershed DIN export was 171 kg km-2 y-1 for the Ipswich and 77 kg km-2 y-1 for the Lamprey. We used the Framework for Aquatic Modeling in the Earth System (FrAMES) to evaluate the relative importance of anthropogenic N loading and river network DIN processing in determining N export from these watersheds. FrAMES is a spatially distributed and time varying coupled hydrologic and biogeochemical model for river networks. We hypothesized that greater N export relative to population density in the Lamprey watershed was due in part to less aquatic N processing caused by interactions among: 1. The distribution of development/sources in the watershed (i.e. mean flow path length N has to travel), and 2. The area and distribution of intact fluvial wetlands in the watershed. We conducted a sensitivity analysis to determine the relative importance of these factors in limiting aquatic N removal in the Lamprey river watershed. Our results suggest that the distribution of loading within a river system has important influence on nutrient export to coastal zones.

  18. The relationship between phytoplankton distribution and water column characteristics in North West European shelf sea waters.

    PubMed

    Fehling, Johanna; Davidson, Keith; Bolch, Christopher J S; Brand, Tim D; Narayanaswamy, Bhavani E

    2012-01-01

    Phytoplankton underpin the marine food web in shelf seas, with some species having properties that are harmful to human health and coastal aquaculture. Pressures such as climate change and anthropogenic nutrient input are hypothesized to influence phytoplankton community composition and distribution. Yet the primary environmental drivers in shelf seas are poorly understood. To begin to address this in North Western European waters, the phytoplankton community composition was assessed in light of measured physical and chemical drivers during the "Ellett Line" cruise of autumn 2001 across the Scottish Continental shelf and into adjacent open Atlantic waters. Spatial variability existed in both phytoplankton and environmental conditions, with clear differences not only between on and off shelf stations but also between different on shelf locations. Temperature/salinity plots demonstrated different water masses existed in the region. In turn, principal component analysis (PCA), of the measured environmental conditions (temperature, salinity, water density and inorganic nutrient concentrations) clearly discriminated between shelf and oceanic stations on the basis of DIN:DSi ratio that was correlated with both salinity and temperature. Discrimination between shelf stations was also related to this ratio, but also the concentration of DIN and DSi. The phytoplankton community was diatom dominated, with multidimensional scaling (MDS) demonstrating spatial variability in its composition. Redundancy analysis (RDA) was used to investigate the link between environment and the phytoplankton community. This demonstrated a significant relationship between community composition and water mass as indexed by salinity (whole community), and both salinity and DIN:DSi (diatoms alone). Diatoms of the Pseudo-nitzschia seriata group occurred at densities potentially harmful to shellfish aquaculture, with the potential for toxicity being elevated by the likelihood of DSi limitation of

  19. Effect of reduced winter precipitation and increased temperature on watershed solute flux, 1988-2002, Northern Michigan

    USGS Publications Warehouse

    Stottlemyer, R.; Toczydlowski, D.

    2006-01-01

    Since 1987 we have studied weekly change in winter (December-April) precipitation, snowpack, snowmelt, soil water, and stream water solute flux in a small (176-ha) Northern Michigan watershed vegetated by 65-85 year-old northern hardwoods. Our primary study objective was to quantify the effect of change in winter temperature and precipitation on watershed hydrology and solute flux. During the study winter runoff was correlated with precipitation, and forest soils beneath the snowpack remained unfrozen. Winter air temperature and soil temperature beneath the snowpack increased while precipitation and snowmelt declined. Atmospheric inputs declined for H+, NO 3- , NH 4+ , dissolved inorganic nitrogen (DIN), and SO 42- . Replicated plot-level results, which could not be directly extrapolated to the watershed scale, showed 90% of atmospheric DIN input was retained in surface shallow (<15 cm deep) soils while SO 42- flux increased 70% and dissolved organic carbon (DOC) 30-fold. Most stream water base cation (C B), HCO 3- , and Cl- concentrations declined with increased stream water discharge, K+, NO 3- , and SO 42- remained unchanged, and DOC and dissolved organic nitrogen (DON) increased. Winter stream water solute outputs declined or were unchanged with time except for NO 3- and DOC which increased. DOC and DIN outputs were correlated with the percentage of winter runoff and stream discharge that occurred when subsurface flow at the plot-level was shallow (<25 cm beneath Oi). Study results suggest that the percentage of annual runoff occurring as shallow lateral subsurface flow may be a major factor regulating solute outputs and concentrations in snowmelt-dominated ecosystems. ?? Springer 2006.

  20. Coseismic liquefaction phenomenon analysis by COSMO-SkyMed: 2012 Emilia (Italy) earthquake

    NASA Astrophysics Data System (ADS)

    Chini, Marco; Albano, Matteo; Saroli, Michele; Pulvirenti, Luca; Moro, Marco; Bignami, Christian; Falcucci, Emanuela; Gori, Stefano; Modoni, Giuseppe; Pierdicca, Nazzareno; Stramondo, Salvatore

    2015-07-01

    The liquefaction phenomenon that occurred in the coseismic phase of the May 20, 2012 Emilia (Italy) earthquake (ML 5.9) is investigated. It was induced by the water pressure increase in the buried and confined sand layers. The level-ground liquefaction was the result of a chaotic ground oscillation caused by the earthquake shaking and the observed failures were due to the upward water flow caused by the excess of pore pressures. We exploited the capability of the differential synthetic aperture radar interferometry (DInSAR) technique to detect soil liquefactions and estimate their surface displacements, as well as the high sensitivity to surface changes of complex coherence, SAR backscattering and intensity correlation. To this aim, a set of four COSMO-SkyMed X-band SAR images, covering the period April 1-June 6, 2012, was used. Geological-geotechnical analysis was also performed in order to ascertain if the detected SAR-based surface effects could be due to the compaction induced by liquefaction of deep sandy layers. In this regards, the results obtained from 13 electrical cone penetrometer tests show the presence of a fine to medium sandy layer at depths, ranging between 9 and 13 m, which probably liquefied during the earthquake, inducing vertical displacements between 3 and 16 cm. The quantitative results from geological-geotechnical analysis and the surface punctual effects measured by DInSAR are in good agreement, even if some differences are present, probably ascribable to the local thickness and depth variability of the sandy layer, or to lack of deformation detection due to DInSAR decorrelation. The adopted approach permitted us to define the extent of the areas that underwent liquefaction and to quantify the local subsidence related to these phenomena. The latter achievement provides useful information that must be considered in engineering practices, in terms of expected vertical deformations.

  1. Landslide Kinematical Analysis through Inverse Numerical Modelling and Differential SAR Interferometry

    NASA Astrophysics Data System (ADS)

    Castaldo, R.; Tizzani, P.; Lollino, P.; Calò, F.; Ardizzone, F.; Lanari, R.; Guzzetti, F.; Manunta, M.

    2015-11-01

    The aim of this paper is to propose a methodology to perform inverse numerical modelling of slow landslides that combines the potentialities of both numerical approaches and well-known remote-sensing satellite techniques. In particular, through an optimization procedure based on a genetic algorithm, we minimize, with respect to a proper penalty function, the difference between the modelled displacement field and differential synthetic aperture radar interferometry (DInSAR) deformation time series. The proposed methodology allows us to automatically search for the physical parameters that characterize the landslide behaviour. To validate the presented approach, we focus our analysis on the slow Ivancich landslide (Assisi, central Italy). The kinematical evolution of the unstable slope is investigated via long-term DInSAR analysis, by exploiting about 20 years of ERS-1/2 and ENVISAT satellite acquisitions. The landslide is driven by the presence of a shear band, whose behaviour is simulated through a two-dimensional time-dependent finite element model, in two different physical scenarios, i.e. Newtonian viscous flow and a deviatoric creep model. Comparison between the model results and DInSAR measurements reveals that the deviatoric creep model is more suitable to describe the kinematical evolution of the landslide. This finding is also confirmed by comparing the model results with the available independent inclinometer measurements. Our analysis emphasizes that integration of different data, within inverse numerical models, allows deep investigation of the kinematical behaviour of slow active landslides and discrimination of the driving forces that govern their deformation processes.

  2. Correlation Between Landforms And Ground Deformation At Nisyros Volcano (Greece)

    NASA Astrophysics Data System (ADS)

    Camiz, S.; Papageorgiou, E.; Poscollieri, M.; Parcharidis, Is.

    2013-12-01

    Relief represents a major element in the characterization of a landscape that can be affected even by slight modifications on its shape, often due to ground deformation. These terrain characteristics are both linked to the topography of a given area, through a Digital Elevation Model (DEM). Topographical attributes, such as slope, aspect, elevation gradients, and others, can be provided by DEM analysis and used further as input to classification methods for defining terrain units. In this study, we explore the relationship between ground deformation and landforms in Nisyros volcano, as ground deformation seems to follow existing geomorphological patterns. Possible correlations between morphological information, collected by classifying landforms on the basis of the ~30 m spatial resolution ASTER Global Digital Elevation Model, and deformation observations resulting from Differential Synthetic Aperture Radar Interferometry (DInSAR) are performed, taking also into consideration the geostructural setting of the study area. Nisyros volcano, located at the eastern part of the active Hellenic Volcanic Arc, was investigated for the time period 2002-2010, during a dormant phase where the topography is mostly described by the geological/tectonic structure. In terms of DInSAR technique, the entire archive of ENVISAT images was used, in both ascending and descending mode, to derive the deformation rates. For the geomorphic consideration Tandem Analysis was implemented through a mixed classification procedure following a principal component analysis applied to local elevation gradients, extracted considering each pixel of the DEM and its nearest neighbours. The projection of the DInSAR results on the obtained factor spaces allows evaluating the homogeneity of the deformation in the pixels belonging to the same classes.

  3. [Nutrient composition changes in coastal waters of Tangshan Bay, Hebei, China under anthropogenic influence].

    PubMed

    Li, Zhi-wei; Cui, Li-tuo

    2016-01-01

    Based on the principal component analysis (PCA), data from 25 marine monitoring stations in Tangshan Bay from 1995 to 2012 were collected to study the change of nutrient composition in Tangshan Bay under anthropogenic influence. Results showed that the inorganic nitrogen (DIN) presented an obvious increase trend in the near 20 years, while the PO₄³⁻-P and SiO₃²⁻-Si presented a decrease trend. The average N:P ratio increased from 3.0 in 1995 to 26.0 in 2012, but the average Si:N ratio decreased, indicating the nutrient structure in seawater had substantially changed in the near 20 years. According to the results of PCA, the change of water quality was identified. The analysis extracted the first two principal components (PC). PC1 was associated with DIN, NO₃⁻-N, NH₄⁺-N, PO₄³⁻-P and NO₂⁻-N, which explained 71.5% of the variance. PC2 was characterized by Chl a and SiO₃²⁻-Si, which explained 21.8% of the variance. It indicated that the water quality of Tangshan bay was closely related to DIN and PO₄³⁻-P. The two principal component scores revealed the interannual change trend of water quality in the Tangshan Bay under anthropogenic influence, which changed from the N limitation before development and at early stage of development (1995-2005) to the P limitation after development (since 2007). The nutrient composition in Tangshan Bay had changed significantly under anthropogenic influence, therefore, special attention is needed on the the change of nutrients in seawater of Tangshan Bay, especially the increase of inorganic nitrogen content. PMID:27228623

  4. The import and export of organic nitrogen species at a Scottish ombrotrophic peatland

    NASA Astrophysics Data System (ADS)

    McKenzie, R. M.; Özel, M. Z.; Cape, J. N.; Drewer, J.; Dinsmore, K. J.; Nemitz, E.; Hamilton, J. F.; Sutton, M. A.; Gallagher, M. W.; Skiba, U.

    2015-01-01

    Dissolved organic nitrogen (DON) can contribute significantly to the overall nitrogen budget, but is not routinely measured in precipitation or stream water. In order to investigate the contribution of DON to deposition and export of N, precipitation, stream and soil water samples were collected from an ombrotrophic peatland and analysed for DON Over a two year period. In wet only deposition DON contributed up to 10% of the total dissolved nitrogen (TDN), 99% in soil water, and 75% in stream water. No correlations were observed between DIN and DON in precipitation stream water or soil water. DIN is an important source of nutrients and in ombrotrophic peatlands, is only deposited via precipitation. Too much nitrogen to a sensitive ecosystem can result in problems with the way in which it is processed, such as an increase in the export of N via nearby water bodies. It is therefore important to monitor N deposition and export. Precipitation DIN showed a loose seasonal pattern, with peak concentrations occurring between January and June, while DON concentrations tended to be lower in the winter months. Stream water DON and NH4+ showed no obvious seasonal pattern but NO3- showed larger concentrations in cooler months and the smallest during warmer months, with the exception of June and July 2010, when concentrations were high. Precipitation and stream DON was qualitatively analysed using GC × GC-NCD. Ten unique compounds were detected, of which only five could be identified: pyrrole, benzonitrile, dodecylamine, N-nitrosodipropylamine and decylamine. Five compounds were present in both precipitation and stream samples: pyrrole, benzonitrile and three unidentified compounds. A more detailed DON speciation may be used to identify sources and pathways of DON.

  5. Fluxes of nutrients from the tropical River Hooghly at the land ocean boundary of Sundarbans, NE Coast of Bay of Bengal, India

    NASA Astrophysics Data System (ADS)

    Mukhopadhyay, S. K.; Biswas, H.; De, T. K.; Jana, T. K.

    2006-08-01

    The Ganges drains much of the southern slopes of the Himalayas and yields high-suspended sediment load during the monsoon. The Hooghly estuary, the first deltaic offshoot of the Ganges and flows through the western part of the Sundarbans mangrove forest is a source for inorganic nutrients due to the high input of litter and sediment associated nutrients that are released during estuarine transport. Dissolved inorganic carbon (DIC), dissolved inorganic nitrogen (DIN), dissolved reactive phosphate (DRP) and silicate budgets and fluxes of the coastal zone of the Hooghly River system have been done by biogeochemical modeling and their annual fluxes were calculated to be 230 × 10 9 mol (2.76 × 10 6 t), 4705 × 10 6 mol (65.8 × 10 3 t), 415 × 10 6 mol (12.8 × 10 3 t), 15.28 × 10 9 mol (42.8 × 10 3 t), respectively. Out of the total transport, monsoonal runoff could account 64% for DIC, 71% for DIN and 76% for both DRP and silicate. Annual load of sediment out of the estuary to the NE coast of Bay of Bengal was estimated to be 65.19 × 10 6 t. During the estuarine transport 7.5% of DIC and 7.0% of silicate were removed while addition for DIN and DRP were found to be 59% and 44%, respectively. Occurrence of light limited conditions and auto-heterotrophic coupling within the estuary results regeneration of nutrients from organic matter originated from external source. Comparison was made with the estimates of nutrient fluxes through the some mangrove dominated Southeast Asian estuaries.

  6. In-stream biotic control on nutrient biogeochemistry in a forested sheadwater tream, West Fork of Walker Branch

    SciTech Connect

    Roberts, Brian J; Mulholland, Patrick J

    2007-01-01

    A growing body of evidence demonstrates the importance of in-stream processing in regulating nutrient export, yet the influence of temporal variability in stream metabolism on net nutrient uptake has not been explicitly addressed. Streamwater DIN and SRP concentrations in Walker Branch, a first-order deciduous forest stream in eastern Tennessee, show a repeated pattern of annual maxima in summer and biannual minima in spring and autumn. Temporal variations in catchment hydrologic flowpaths result in lower winter and higher summer nutrient concentrations, but do not explain the spring and autumn nutrient minima. Ambient nutrient uptake rates were measured 2-3 times per week over an 18-mo period and compared to daily rates of gross primary production (GPP) and ecosystem respiration (ER) to examine the influence of in-stream biotic activity on nutrient export. GPP and ER rates explained 85% of the variation in net DIN retention with high net NO3- uptake (and lower net NH4+ release) rates occurring during spring and autumn and net DIN release in summer. Diel nutrient concentration patterns were examined several times throughout the year to determine the relative importance of autotrophic and heterotrophic activity on net nutrient uptake. High spring GPP corresponded to daily decreases in NO3- over the illuminated hours resulting in high diel NO3- amplitude which dampened as the canopy closed. GPP explained 91% of the variance in diel NO3- amplitude. In contrast, the autumn nutrient minima was largely explained by heterotrophic respiration since GPP remained low and little diel NO3- variation was observed during the autumn.

  7. A Molecular Bar-Coded DNA Repair Resource for Pooled Toxicogenomic Screens

    PubMed Central

    Rooney, John P.; Patil, Ashish; Zappala, Maria R.; Conklin, Douglas S.; Cunningham, Richard P.; Begley, Thomas J.

    2008-01-01

    DNA damage from exogenous and endogenous sources can promote mutations and cell death. Fortunately, cells contain DNA repair and damage signalling pathways to reduce the mutagenic and cytotoxic effects of DNA damage. The identification of specific DNA repair proteins and the coordination of DNA repair pathways after damage has been a central theme to the field of Genetic Toxicology and we have developed a tool for use in this area. We have produced 99 molecular bar-coded Escherichia coli gene-deletion mutants specific to DNA repair and damage signalling pathways, and each bar-coded mutant can be tracked in pooled format using bar-code specific microarrays. Our design adapted bar-codes developed for the Saccharomyces cerevisiae Gene Deletion Project, which allowed us to utilize an available microarray product for pooled gene-exposure studies. Microarray-based screens were used for en masse identification of individual mutants sensitive to methyl methanesulfonate (MMS). As expected, gene deletion mutants specific to direct, base excision, and recombinational DNA repair pathways were identified as MMS-sensitive in our pooled assay, thus validating our resource. We have demonstrated that molecular bar-codes designed for S. cerevisiae are transferable to E. coli, and that they can be used with pre-existing microarrays to perform competitive growth experiments. Further, when comparing microarray to traditional plate-based screens both over-lapping and distinct results were obtained, which is a novel technical finding, with discrepancies between the two approaches explained by differences in output measurements (DNA content verse cell mass). The microarray-based classification of Δtag and ΔdinG cells as depleted after MMS exposure, contrary to plate-based methods, led to the discovery that Δtag and ΔdinG cells show a filamentation phenotype after MMS exposure, thus accounting for the discrepancy. A novel biological finding is the observation that while ΔdinG cells

  8. Geochemical Rate/RNA Integration Study (GRIST): A Pilot Field Experiment for Inter-Calibration of Biogeochemistry and Nucleic Acid Measurements Final Report

    SciTech Connect

    Bronk, Deborah

    2007-01-08

    The Geochemical Rate/RNA Integration Study (GRIST) project sought to correlate biogeochemical flux rates with measurements of gene expression and mRNA abundance to demonstrate the application of molecular approaches to estimate the presence and magnitude of a suite of biogeochemical processes. The study was headed by Lee Kerkhoff of Rutgers University. In this component of the GRIST study, we characterized ambient nutrient concentrations and measured uptake rates for dissolved inorganic nitrogen (DIN, ammonium, nitrate and nitrite) and dissolved organic nitrogen (urea and dissolved free amino acids) during two diel studies at the Long-Term Ecosystem Observatory (LEO-15) on the New Jersey continental shelf.

  9. Adopting Industry Standards for Control Systems Within Advanced Life Support

    NASA Technical Reports Server (NTRS)

    Young, James Scott; Boulanger, Richard

    2002-01-01

    This paper gives a description of OPC (Object Linking and Embedding for Process Control) standards for process control and outlines the experiences at JSC with using these standards to interface with I/O hardware from three independent vendors. The I/O hardware was integrated with a commercially available SCADA/HMI software package to make up the control and monitoring system for the Environmental Systems Test Stand (ESTS). OPC standards were utilized for communicating with I/O hardware and the software was used for implementing monitoring, PC-based distributed control, and redundant data storage over an Ethernet physical layer using an embedded din-rail mounted PC.

  10. Clasificación orbital

    NASA Astrophysics Data System (ADS)

    Carpintero, D. D.; Aguilar, L. A.

    Presentamos un método para clasificar órbitas generales en potenciales de 2 y 3 dimensiones, basado en el concepto de dinámica espectral introducido por Binney y Spergel (ApJ 252, 308, 1982). Este método distingue correctamente órbitas regulares e irregulares, familias de órbitas (cajas, tubos, etc.), y resonancias, aún cuando son de alto orden o de rango mayor que 1. Se propone asimismo una nueva nomenclatura para la clasificación de órbitas.

  11. Long term changes in the ecosystem in the northern South China Sea during 1976-2004

    NASA Astrophysics Data System (ADS)

    Ning, X.; Lin, C.; Hao, Q.; Liu, C.; Le, F.; Shi, J.

    2009-10-01

    Physical and chemical oceanographic data were obtained by seasonal monitoring along a transect (Transect N) in the northern South China Sea (nSCS) during 1976-2004. Fluctuations of DIN (dissolved inorganic nitrogen), seawater temperature (SST and Tav - average temperature of the water column), N:P ratio and salinity (Sav and S200 - salinity at the 200 m layer) exhibited an increasing trend, while those of T200, DO, P, Si, Si:N and SSS exhibited a decreasing trend. The annual rates of change in DIN, DO, T and S revealed pronounced changes, and the climate trend coefficients, which was defined as the correlation coefficient between the time series of an environmental parameter and the nature number (namely 1,2,3,......n), were 0.38 to 0.89 and significant (p≤0.01 to 0.05). Our results also showed that the ecosystem has obviously been influenced by the positive trends of both SST and DIN, and negative trends of both DO and P. For example, before 1997, DIN concentrations in the upper layer were very low and N:P ratios were less than half of the Redfield ratio of 16, indicating potential N limitation. However after 1997, all Si:P ratios were >22 and the Nav:Pav was close to the Redfield ratio, indicating potential P limitation, and therefore N limitation has been reduced after 1997. Ecological investigation shows that there have been some obvious responses of the ecosystems to the long-term environmental changes in the nSCS. Chlorophyll-a concentration, primary production, phytoplankton abundance, benthic biomass, cephalopod catch and demersal trawl catch have increased. But phosphorus depletion in upper layer may be related to the shift in the dominant species from diatoms to dinoflagellates and cyanophytes. The ecosystem response was induced by not only anthropogenic activities, but also global climate change, e.g. ENSO. The effects of climate change on the nSCS were mainly through changes in the monsoon winds, and physical-biological oceanography coupling processes

  12. Long term changes in the ecosystem in the northern South China Sea during 1976 2004

    NASA Astrophysics Data System (ADS)

    Ning, X.; Lin, C.; Hao, Q.; Liu, C.; Le, F.; Shi, J.

    2008-09-01

    Physical and chemical oceanographic data were obtained by seasonal monitoring along Transect N in the northern South China Sea (nSCS) during 1976 2004. Fluctuations of DIN (dissolved inorganic nitrogen), seawater temperature (SST and Tav average temperature of the water column), N:P ratio and salinity (Sav and S200 salinity at the 200 m layer) exhibited an increasing trend, while those of T200, DO, P, Si, Si:N and SSS exhibited a decreasing trend. The annual rates of change in DIN, DO, T and S revealed pronounced changes, and the climate trend coefficients Rxt, which was defined as the correlation coefficient between the time series of an environmental parameter and the nature number, were 0.38 to 0.89 and significant (p≤0.01 to 0.05). Our results also showed that the ecosystem has obviously been influenced by the positive trends of both SST and DIN, and negative trends of both DO and P, e.g. before 1997, DIN concentrations in the upper layer were very low and N:P ratios were less than half of the Redfield ratio of 16, indicating potential N limitation. However, after 1997, all Si:P ratios were >22 and the Nav:Pav was close to the Redfield ratio, indicating potential P limitation, and therefore N limitation has been reduced after 1997. Ecological investigation shows that there have been some improved responses of the ecosystems to the long-term environmental changes in the nSCS, and chlorophyll-a concentration, primary production, phytoplankton abundance, benthic biomass, cephalopod catch and demersal trawl catch have increased. But phosphorus depletion in upper layer may be related to the shift in the dominant species from diatoms to dinoflagellates and cyanophytes. The ecosystem response was induced by not only anthropogenic activities, but also global climate change, e.g. pronounced responses to ENSO. The effects of climate change on the nSCS were mainly through changes in the monsoon winds, and physical-biological oceanography coupling processes.

  13. Impact of intensified irrigated agriculture and climate change on nitrogen loading in the Amu Darya drainage basin, Central Asia

    NASA Astrophysics Data System (ADS)

    Törnqvist, Rebecka; Jarsjö, Jerker

    2013-04-01

    Pollutant loading and water losses by evapotranspiration are two main problems of intensified irrigated agricultural in arid and semi-arid regions. Climatic changes can further increase water losses and alter transport pathways for contaminants and nutrients. Identification of dominant processes that control nitrogen (N) loading in the highly managed Amu Darya Drainage Basin (ADRB), the largest sub-basin in the Aral Sea Drainage Basin (ASDB), is considered by looking at a 40-years (1960-2000) data record of dissolved inorganic nitrogen (DIN). Furthermore, hydrologic distributed modelling was used to investigate how N transport pathways and travel times have changed with past irrigation expansion, and is likely to change further in response to projected future hydro-climatic trends. River discharge has decreased drastically during the considered 40-years period in ADRB. Future climate and land-use projections show that downstream regions even are at risk of total surface water depletion within a future 30-years period. Decreasing riverine DIN concentration was observed near the Aral Sea outlet despite increasing N fertilizer application throughout the 40-years period. The reduction in concentrations could not be explained by increased N crop uptake, improved fertilization application or improved irrigation efficiency. Instead, this must primarily be due to a considerable increase in reuse in irrigation which extends the flow-path lengths and enhances N retention. A relationship between increased recirculation ratio (defined as the basin-scale return flow divided by the outflow) and decreased Cout/Cin ratio was developed, and shown to be valid for a relatively wide uncertainty range. An observed six-fold decrease in DIN load was primarily, but not exclusively, due to the drastic river flow reduction. Consequently, N accumulation in the soil-groundwater system has accelerated since the N fertilization has been maintained high throughout the period of considerable

  14. PACS clinical experience at Georgetown University.

    PubMed

    Horii, S C; Mun, S K; Levine, B; Lo, B; Garra, B S; Zeman, R K; Freedman, M; Leftridge, C; Schellinger, D; Keyes, J

    1991-01-01

    Georgetown University Hospital has been operating an image management and communications system (IMACS or PACS) for three-and-a-half years. This work was initially funded under the Army Medical Research and Development Command Digital Imaging Network Systems (DINS) project. The system was taken from a research system supporting only radiology tasks to one extended to clinical use, and has been used in clinical work for two-and-a-half years. This paper will summarize our PACS clinical experience and will describe the operational features implemented and those still necessary. PMID:1913567

  15. [Organized emergency medical aid (author's transl)].

    PubMed

    Sefrin, P

    1975-02-01

    The decision to have medical attendance at accidents has changed the structure of the entire accident rescue service. Standardization (DIN) has provided the basis for efficient equipment and new legislation regarding the special training of orderlies as ambulancemen has ensured an highly expert emergency service personnel. The manifold duties of the emergency service physician are outlined. To perform them competently he needs not only a thorough knowledge of the various branches of medicine, especially of resuscitation and maintenance of vital function, but he must also be acquainted with techniques for the rescue and extrication of casualties and with radio-communication. Suggestions are made for the training of emergency medical personnel. PMID:1233465

  16. On the Cutting Performance of Coated HSS Taps When Machining of Austenitic Steel

    NASA Astrophysics Data System (ADS)

    Sliwkova, Petra; Piska, Miroslav

    2014-12-01

    The paper deals with a quality of the PVD coated HSS taps when cutting the stainless austenitic chromiumnickel non-stabilized steel DIN 1.4301 (X5CrNi 18-10). The main attention is focused on the analysis of loading (cutting moment, specific energy) of the HSS taps by means of pieso-electrical dynamometer Kistler 9272 and the relation between the quality of duplex and triplex PVD coatings and their effects on the quality of machined thread surfaces and tool life of the taps. The results showed a safe and stabilized cutting with acceptable quality of threads for HSSE with the TiN+TiCN+DLC coating.

  17. Effects of Deepwater Algae on C-N-P Cycling in Permeable Sediments

    NASA Astrophysics Data System (ADS)

    Sansone, F. J.; Spalding, H. L.; Smith, C. M.

    2007-12-01

    Extensive deepwater (50-100 m depth) meadows of native and introduced macroalgae occur offshore of the main Hawaiian Islands. Sediment porewater (to 25 cm sediment depth) and overlying seawater were collected during September and December 2004, and November and December 2006 from 13 vegetated and non-vegetated deepwater sites on sand and muddy-sand seafloors offshore from Oahu to Kaho'olawe. Porewater dissolved nutrient concentrations were comparable to those in nearshore permeable (sandy) sediments, with highly elevated concentrations at sediment depths available to algal holdfasts. Maximum observed concentrations were: 2.3 uM phosphate, 30 uM nitrate, 0.65 uM nitrite, 105 uM ammonium, and 88 uM silica. The sediment organic matter content was not correlated with the presence or absence of macroalgae. Vegetated sediments were consistently more oxidizing than non-vegetated sediments, judged from (nitrate + nitrite)/ammonium ratios. Halimeda-vegetated sediments had low DIN levels compared to other sites, suggesting algal uptake. These sands also exhibited dissolved inorganic carbon depletion at depth, perhaps also related to algal metabolism. In contrast, Udotea-vegetated sediments had high levels of DIN, indicating little or no algal DIN uptake and/or the presence of active N-fixation; these sands also had no DIC depletion at depth. Udotea-vegetated sediments also appeared highly oxidizing, evidenced by very high levels of dissolved nitrate and nitrite, along with low levels of ammonium. The calculated tracer N* was used to verify the conclusions regarding net DIN uptake; a similar calculated tracer, computed using Redfield-type N:Si ratios, was used to confirm the N* results. Unvegetated, nutrient- and organic-rich sandy mud along the flanks of Haleakala volcano showed near complete dissolved phosphate removal, perhaps due to adsorption onto freshly weathered iron oxy-hydroxides. Such phosphate removal was not seen at other sites, including a nearby non

  18. Solar cell modules for plasma interaction evaluation

    NASA Technical Reports Server (NTRS)

    1981-01-01

    A plasma interaction analysis in support of the solar electric propulsion subsystem examined the effects of a large high voltage solar array interacting with an ion thruster produced plasma. Two solar array test modules consisting of 36 large area wraparound contact solar cells welded to a flexible Kapton integrated circuit substrate were abricated. The modules contained certain features of the effects of insulation, din-holes, and bonding of the cell to the substrate and a ground plane. The possibility of a significant power loss occurring due to the collection of charged particles on the solar array interconnects was the focus of the research.

  19. Mine roof geology information system

    SciTech Connect

    Peng, S.S.; Sasaoka, T.; Tang, D.X.; Wilson, Y.; Wilson, G.

    2005-05-01

    A project sponsored by the US Department of Energy under the Industry of Future (Mining) program was initiated five years ago. In this project a patented drill control unit (DCU) installed DIN. the J.H. Flecher & Co.'s roof bolter was used to record the drilling parameter for experiments conducted in the mines and laboratory. Today, the drilling parameters have been recorded for more than 1,000 roof bolt holes. This article summarizes the results to date including the methods for determining quantitatively the location of voids/fractures and estimation of roof rock strength from the recorded roof bolter drilling parameters. 8 figs., 2 tabs.

  20. Nutrient inputs from the watershed and coastal eutrophication in the Florida Keys

    SciTech Connect

    LaPointe, B.E. ); Clark, M.W. )

    1992-12-01

    Widespread use of septic tanks in the Florida Keys increase the nutrient concentrations of limestone ground waters that discharge into shallow nearshore waters, resulting in coastal eutrophication. This study characterizes watershed nutrient inputs, transformations, and effects along a land-sea gradient stratified into four ecosystems that occur with increasing distance from land: manmade canal systems, seagrass meadows, patch reefs, and offshore bank reefs. Soluble reactive phosphorus (SRP), the primary limiting nutrient, was significantly elevated in canal systems, while dissolved inorganic nitrogen (DIN; NH[sub 4][sup =] and NO[sub 3][sup [minus

  1. The yield of chlorophyll from nitrogen: a comparison between the shallow Ria Formosa lagoon and the deep oceanic conditions at Sagres along the southern coast of Portugal

    NASA Astrophysics Data System (ADS)

    Edwards, Vivien; Icely, John; Newton, Alice; Webster, Ruth

    2005-02-01

    The yield of chlorophyll from dissolved inorganic nitrogen (DIN) has been shown to be a potentially useful parameter for predicting eutrophication, particularly, in the northerly, coastal waters of the North East Atlantic (NEA). This study investigates whether this parameter might also be appropriate for the southerly, coastal waters of the NEA. Nitrogen enrichment experiments were carried out using microcosms to determine the microplanktonic yield of chlorophyll from DIN in waters from the Ria Formosa (April 2002) and from Sagres (September 2002) on the south coast of Portugal. Continuous culture techniques enabled experiments to be run for 7 days after enrichment so that changes in the cumulative yield over time could be calculated. Yields from the Sagres experiment were consistently higher than those from the Ria Formosa experiment, with respective maximum yields of 4.7 and 2.1 μg chl (μmol N) -1, and respective steady-state yields of 3.1 and 0.9 μg chl (μmol N) -1. In addition, regressions carried out on historical data sets from the two study sites showed poor correlation between chlorophyll and nitrate. Other differences between the microcosm experiments at the two sites, included: background concentrations of DIN, silicate and phosphate that were, respectively, 5.6 μM, 8.1 μM, and 0.3 μM higher in the Ria; chlorophyll concentrations at Sagres that were double those of the Ria; accumulation of particulate nitrogen that was both more rapid and more substantial at Sagres; a different community structure for the diatoms at the two sites; more numerous autotrophic dinoflagellates, flagellates and cyanobacteria, as well as more numerous protozoan grazers, at Sagres. These differences may explain why the yield of chlorophyll from DIN at Sagres is one of the highest reported in the literature. This yield parameter requires further study under a range of seasonal conditions and with a range of microplankton communities before it could be considered useful for

  2. Poultry manure runoff and its influence on fluorescence characteristics of dissolved organic matter (DOM)

    NASA Astrophysics Data System (ADS)

    Singh, S.; Dutta, S.; Inamdar, S. P.

    2013-12-01

    Land application of poultry manure as a substitute for synthetic fertilizer is a common practice in states like Delaware which have a surplus of this animal waste. However, this practice can generate large amounts of labile DOM and nutrients in agricultural runoff that can cause eutrophication of downstream aquatic ecosystems. We determined the concentrations of dissolved organic carbon (DOC) and dissolved inorganic nitrogen (DIN) and the quality of DOM for a cropland receiving poultry manure in the coastal plain soils of Delaware. Manure was applied at the rate of 9 Mg ha-1 in the spring (March 10) of 2010 to an agricultural field planted in corn. Sampling was performed for surface runoff and soil waters at four landscape positions - field edge, upper and lower riparian zones and the stream. Sampling was conducted for eight storm events, one before manure application and seven after (March through July spanning over 100 days). DOM quality was characterized using spectrofluorometric techniques and the development of a site-specific PARAFAC model. DOC and DIN concentrations in surface runoff ranged from 18.1 to 77.2 mg/l and 4.2 to 22.6 mg/l, respectively. The percent of protein-like and humic-like DOM in surface runoff ranged between 3.9 to 23.5% and 12.3 to 41.6%, respectively. Highest concentrations of DOC and DIN were observed at the field edge and lowest in the stream. Protein-like and humic-like DOM decreased from the field edge to stream in surface runoff and soil waters. Temporally, both humic-like and protein-like DOM showed significant increases in storm runoff following manure application. After manure application, humic-like DOM increased by 70% while protein-like DOM increased by more than 200% in surface runoff indicating elevated content of labile DOM in poultry manure. These concentrations remained high for more than 60 days following manure application. Protein-like DOM was significantly correlated with nitrate-nitrogen (r = 0.43; p < 0

  3. Femtoquímica: reacciones en tiempo real, ?`Posibles aplicaciones en Astrofísica?

    NASA Astrophysics Data System (ADS)

    Douhal, Abderrazzak

    En esta conferencia, se mostrarán resultados de medidas en escala del femtosegundo por la primera vez obtenidos en España. Los sistemas elegidos muestran dinámicas que varían de 50 fs a centenas de picosegundo. La técnica usada (sumación de frecuencia) permite ver como ocurren las reacciones químicas y biológicas en tiempo real de reacciones. Su aplicación al estudio de las reacciones fotónicas en el espacio es evidente.

  4. Consequences of winter upwelling events on biogeochemical and phytoplankton patterns in a western Galician ria (NW Iberian peninsula)

    NASA Astrophysics Data System (ADS)

    Prego, Ricardo; Guzmán-Zuñiga, Dafne; Varela, Manuel; deCastro, Maite; Gómez-Gesteira, Moncho

    2007-07-01

    The consequences of two upwelling events in mid- (MW) and late (LW) winter on biogeochemical and phytoplankton patterns were studied in the Pontevedra Ria and compared with the patterns measured under typical winter conditions and under a summer upwelling event. Thermohaline patterns measured during the mid-winter upwelling event (MW-up) revealed the intrusion of saltier seawater (35.9) into the ria associated with the Iberian Poleward Current (IPC). During the late-winter upwelling event (LW-up), the seawater which had welled up into the ria showed characteristics of the Eastern North Atlantic Central Water mass (ENACW). In both cases the measured water residence time (4 days during MW-up and 10 days during LW-up) was related to both meteorological and fluvial forcing. This residence time contrasts with that of summer upwelling (7 days) and with that estimated under unfavorable upwelling atmospheric conditions (2-4 weeks). During MW-up, the ria became poor in nutrients due to continental freshwater dilution, associated with the shorter residence time of the water, and the intrusion of IPC, which is a water body poor in nutrient salts: 2.9 μM of nitrate, 0.1 μM of phosphate and 1.5 μM of silicate. During this event, the ria exported 3.4 mol DIN s -1, compared with 6.9 mol DIN s -1 in non-upwelling conditions. Phytoplankton showed a uniform distribution throughout the ria, as during unfavorable upwelling conditions, and was characterized by the dominance of diatoms, mainly Nitzschia longissima and Skeletonema costatum. During LW-up, a nutrient depletion in the photic layer also occurred, but as a result of a phytoplankton spring bloom developing at this time. The ria was a nutrient trap where 4.1 mol DIN s -1 were processed by photosynthesis. This budget is three times higher than the one under non-upwelling conditions. In contrast with the MW-up, which had no effect on primary production, during LW-up the ria became more productive, although not as productive as

  5. Final Report Navajo Transmission Project (NTP)

    SciTech Connect

    Bennie Hoisington; Steven Begay

    2006-09-14

    The Diné Power Authority is developing the Navajo Transmission Project (NTP) to relieve the constraints on the transmission of electricity west of the Four Corners area and to improve the operation flexibility and reliability of the extra-high-voltage transmission system in the region. The NTP creates the wholesale transmission capacity for more economical power transfers, sales, and purchases in the region. It will facilitate the development of Navajo energy resources, improve economic conditions on the Navajo Nation as well as allow DPA to participate in the western electrical utility industry.

  6. Spatial patterns of atmospheric deposition of nitrogen and sulfur using ion-exchange resin collectors in Rocky Mountain National Park, USA

    NASA Astrophysics Data System (ADS)

    Clow, David W.; Roop, Heidi A.; Nanus, Leora; Fenn, Mark E.; Sexstone, Graham A.

    2015-01-01

    Lakes and streams in Class 1 wilderness areas in the western United States (U.S.) are at risk from atmospheric deposition of nitrogen (N) and sulfur (S), and protection of these resources is mandated under the Federal Clean Air Act and amendments. Assessment of critical loads, which are the maximum exposure to pollution an area can receive without adverse effects on sensitive ecosystems, requires accurate deposition estimates. However, deposition is difficult and expensive to measure in high-elevation wilderness, and spatial patterns in N and S deposition in these areas remain poorly quantified. In this study, ion-exchange resin (IER) collectors were used to measure dissolved inorganic N (DIN) and S deposition during June 2006-September 2007 at approximately 20 alpine/subalpine sites spanning the Continental Divide in Rocky Mountain National Park. Results indicated good agreement between deposition estimated from IER collectors and commonly used wet + dry methods during summer, but poor agreement during winter. Snowpack sampling was found to be a more accurate way of quantifying DIN and S deposition during winter. Summer DIN deposition was significantly greater on the east side of the park than on the west side (25-50%; p ≤ 0.03), consistent with transport of pollutants to the park from urban and agricultural areas to the east. Sources of atmospheric nitrate (NO3-) were examined using N isotopes. The average δ15N of NO3- from IER collectors was 3.5‰ higher during winter than during summer (p < 0.001), indicating a seasonal shift in the relative importance of regional NOx sources, such as coal combustion and vehicular sources of atmospheric NO3-. There were no significant differences in δ15N of NO3- between east and west sides of the park during summer or winter (p = 0.83), indicating that the two areas may have similar sources of atmospheric NO3-. Results from this study indicate that a combination of IER collectors and snowpack sampling can be used to

  7. A Synergy Method to Improve Ensemble Weather Predictions and Differential SAR Interferograms

    NASA Astrophysics Data System (ADS)

    Ulmer, Franz-Georg; Adam, Nico

    2015-11-01

    A compensation of atmospheric effects is essential for mm-sensitivity in differential interferometric synthetic aperture radar (DInSAR) techniques. Numerical weather predictions are used to compensate these disturbances allowing a reduction in the number of required radar scenes. Practically, predictions are solutions of partial differential equations which never can be precise due to model or initialisation uncertainties. In order to deal with the chaotic nature of the solutions, ensembles of predictions are computed. From a stochastic point of view, the ensemble mean is the expected prediction, if all ensemble members are equally likely. This corresponds to the typical assumption that all ensemble members are physically correct solutions of the set of partial differential equations. DInSAR allows adding to this knowledge. Observations of refractivity can now be utilised to check the likelihood of a solution and to weight the respective ensemble member to estimate a better expected prediction. The objective of the paper is to show the synergy between ensemble weather predictions and differential interferometric atmospheric correction. We demonstrate a new method first to compensate better for the atmospheric effect in DInSAR and second to estimate an improved numerical weather prediction (NWP) ensemble mean. Practically, a least squares fit of predicted atmospheric effects with respect to a differential interferogram is computed. The coefficients of this fit are interpreted as likelihoods and used as weights for the weighted ensemble mean. Finally, the derived weighted prediction has minimal expected quadratic errors which is a better solution compared to the straightforward best-fitting ensemble member. Furthermore, we propose an extension of the algorithm which avoids the systematic bias caused by deformations. It makes this technique suitable for time series analysis, e.g. persistent scatterer interferometry (PSI). We validate the algorithm using the well known

  8. El problema de estabilidad de los sistemas Hamiltonianos multidimensionales

    NASA Astrophysics Data System (ADS)

    Cincotta, P. M.

    Se revisarán los aspectos básicos del problema de estabilidad de sistemans Hamiltonianos N-dimensionales, haciendo especial énfasis en los posibles mecanismos que dan lugar a la aparición de ``caos": overlap de resonancias, difusión de Arnol'd y otros procesos difusivos alternativos. Se mencionarán los aspectos aún no resueltos sobre la estabilidad de los sistemas con N > 2. Finalmente, se discutirá cuáles de estos mecanismos podrían tener alguna relevancia en la dinámica de sistemas estelares y planetarios.

  9. Can analyses of electronic patient records be independently and externally validated? Study 2—the effect of β-adrenoceptor blocker therapy on cancer survival: a retrospective cohort study

    PubMed Central

    Springate, David A; Ashcroft, Darren M; Kontopantelis, Evangelos; Doran, Tim; Ryan, Ronan; Reeves, David

    2015-01-01

    Objectives To conduct a fully independent, external validation of a research study based on one electronic health record database using a different database sampling from the same population. Design Retrospective cohort analysis of β-blocker therapy and all-cause mortality in patients with cancer. Setting Two UK national primary care databases (PCDs): the Clinical Practice Research Datalink (CPRD) and Doctors’ Independent Network (DIN). Participants CPRD data for 11 302 patients with cancer compared with published results from DIN for 3462 patients; study period January 1997 to December 2006. Primary and secondary outcome measures All-cause mortality: overall; by treatment subgroup (β-blockers only, β-blockers plus other blood pressure lowering medicines (BPLM), other BPLMs only); and by cancer site. Results Using CPRD, β-blocker use was not associated with mortality (HR=1.03, 95% CI 0.93 to 1.14, vs patients prescribed other BPLMs only), but DIN β-blocker users had significantly higher mortality (HR=1.18, 95% CI 1.04 to 1.33). However, these HRs were not statistically different (p=0.063), but did differ for patients on β-blockers alone (CPRD=0.94, 95% CI 0.82 to 1.07; DIN=1.37, 95% CI 1.16 to 1.61; p<0.001). Results for individual cancer sites differed by study, but only significantly for prostate and pancreas cancers. Results were robust under sensitivity analyses, but we could not be certain that mortality was identically defined in both databases. Conclusions We found a complex pattern of similarities and differences between databases. Overall treatment effect estimates were not statistically different, adding to a growing body of evidence that different UK PCDs produce comparable effect estimates. However, individually the two studies lead to different conclusions regarding the safety of β-blockers and some subgroup effects differed significantly. Single studies using even internally well-validated databases do not guarantee generalisable results

  10. Microscopic properties of liquid gallium from quasi-elastic neutron scattering experiments

    NASA Astrophysics Data System (ADS)

    Blagoveshchenskii, N. M.; Novikov, A. G.; Puchkov, A. V.; Savostin, V. V.

    2014-11-01

    The results of the neutron diffraction study of liquid gallium on the DIN-2PI spectrometer (IBR-2 reactor, JINR, Dubna) have been discussed. The analysis of the experimental data has provided temperature dependences of diffusion and relaxation characteristics of liquid gallium in the temperature range of 313-793 K. It has been found that an increase in the temperature is accompanied by the gradual deviation of the temperature dependence of the self-diffusion coefficient from the known dependences obtained from the data on viscosity. Such a behavior is explained by the beginning clustering of liquid gallium induced by the prevailing covalent character of interatomic bonds.

  11. Collective microdynamics of liquid lithium: An inelastic neutron scattering study

    NASA Astrophysics Data System (ADS)

    Blagoveshchenskiĭ, N. M.; Novikov, A. G.; Savostin, V. V.

    2010-05-01

    A portion of the dispersion curve for collective modes in liquid lithium has been constructed from experimental data on inelastic scattering of slow neutrons obtained on the DIN-2PI neutron spectrometer (IBR-2 reactor, Joint Institute for Nuclear Research, Dubna, Russia). Measurements have been performed at a temperature of 500 K ( T m (Li) = 453.7 K). The coherent scattering component has been separated from the experimental spectra and analyzed. Information on the characteristics of collective excitations in liquid lithium has been derived.

  12. Fate of excess phosphorus in the Baltic Sea: A real driving force for cyanobacterial blooms?

    NASA Astrophysics Data System (ADS)

    Raateoja, M.; Kuosa, H.; Hällfors, S.

    2011-02-01

    The wintertime ratio of inorganic dissolved nitrogen to phosphorus (DIN/DIP) in the surface waters of the Baltic Sea is typically below the molar Redfield ratio of 16, which expresses the presence of an excess inorganic dissolved phosphorus (eDIP) reserve compared to DIN. We assessed the role of the vernal phytoplankton bloom period (VPBP) in the consumption of the potential wintertime eDIP reserve, and the role of eDIP after the VPBP as a nutritional agent for the summertime growth of the cyanobacteria in the Baltic Sea. We employed a high-frequency dataset collected by the unattended monitoring systems on board merchant ships. The dataset encompasses the Baltic basins from the Arkona Basin to the Western Gulf of Finland and the time period from 1993 to 2009. All the observed values of the wintertime DIN/DIP ratio were below the molar Redfield ratio; the ratio showed a declining trend during the study, suggesting that there is a pronounced wintertime stock of the potential eDIP in the waters of the Baltic Sea, and that this stock has lately increased in magnitude. The VPBP took up excessively DIP to DIN than calculated according to the uptake in the molar Redfield ratio, thus reducing the potential eDIP reserve. On average, 59% of the potential eDIP reserve was left in the water after the VPBP as eDIP. eDIP was typically exhausted in the time frame early June-early July, matching well the timing of the appearance of cyanobacteria in substantial numbers in the water-column. eDIP clearly fueled the cyanobacterial growth in every instance in which it was possible to clarify their relationship. The cyanobacteria must still have another DIP source than eDIP to form extensive late-summer blooms, except in the western Gulf of Finland, where eDIP remained detectable up to early August. The annual role of eDIP for cyanobacterial growth depends greatly on the weather of late spring and early summer: this may sometimes launch the cyanobacterial growth much earlier than is

  13. Nutrient fluctuations in the Quatipuru river: A macrotidal estuarine mangrove system in the Brazilian Amazonian basin

    NASA Astrophysics Data System (ADS)

    Pamplona, Fábio Campos; Paes, Eduardo Tavares; Nepomuceno, Aguinaldo

    2013-11-01

    The temporal and spatial variability of dissolved inorganic nutrients (NO3-, NO2-, NH4+, PO43- and DSi), total nitrogen (TN), total phosphorus (TP), nutrient ratios, suspended particulate matter (SPM) and Chlorophyll-a (Chl-a) were evaluated for the macrotidal estuarine mangrove system of the Quatipuru river (QUATIES), east Amazon coast, North Brazil. Temporal variability was assessed by fortnightly sampling at a fixed station within the middle portion of the estuary, from November 2009 to November 2010. Spatial variability was investigated from two field surveys conducted in November 2009 (dry season) and May 2010 (rainy season), along the salinity gradient of the system. The average DIN (NO3- + NO2- + NH4+) concentration of 9 μM in the dry season was approximately threefold greater in comparison to the rainy season. NH4+ was the main form of DIN in the dry season, while NO3- predominated in the rainy season. The NH4+ concentrations in the water column during the dry season are largely attributed to release by tidal wash-out of the anoxic interstitial waters of the surficial mangrove sediments. On the other hand, the higher NO3- levels during the wet season, suggested that both freshwater inputs and nitrification processes in the water column acted in concert. The river PO43- concentrations (DIP < 1 μM) were low and similar throughout the year. DIN was thus responsible for the major temporal and spatial variability of the dissolved DIN:DIP (N:P) molar ratios and nitrogen corresponded, in general, to the prime limiting nutrient for the sustenance of phytoplankton biomass in the estuary. During the dry season, P-limitation was detected in the upper estuary. PO43- adsorption to SPM was detected during the rainy season and desorption during the dry season along the salinity gradient. In general, the average Chl-a level (14.8 μg L-1) was 2.5 times higher in the rainy season than in the dry season (5.9 μg L-1). On average levels reached maxima at about 14 km from

  14. Spatial patterns of atmospheric deposition of nitrogen and sulfur using ion-exchange resin collectors in Rocky Mountain National Park, USA

    USGS Publications Warehouse

    Clow, David W.; Roop, Heidi; Nanus, Leora; Fenn, Mark; Sexstone, Graham A.

    2015-01-01

    Lakes and streams in Class 1 wilderness areas in the western United States (U.S.) are at risk from atmospheric deposition of nitrogen (N) and sulfur (S), and protection of these resources is mandated under the Federal Clean Air Act and amendments. Assessment of critical loads, which are the maximum exposure to pollution an area can receive without adverse effects on sensitive ecosystems, requires accurate deposition estimates. However, deposition is difficult and expensive to measure in high-elevation wilderness, and spatial patterns in N and S deposition in these areas remain poorly quantified. In this study, ion-exchange resin (IER) collectors were used to measure dissolved inorganic N (DIN) and S deposition during June 2006–September 2007 at approximately 20 alpine/subalpine sites spanning the Continental Divide in Rocky Mountain National Park. Results indicated good agreement between deposition estimated from IER collectors and commonly used wet + dry methods during summer, but poor agreement during winter. Snowpack sampling was found to be a more accurate way of quantifying DIN and S deposition during winter. Summer DIN deposition was significantly greater on the east side of the park than on the west side (25–50%; p ≤ 0.03), consistent with transport of pollutants to the park from urban and agricultural areas to the east. Sources of atmospheric nitrate (NO3−) were examined using N isotopes. The average δ15N of NO3− from IER collectors was 3.5‰ higher during winter than during summer (p < 0.001), indicating a seasonal shift in the relative importance of regional NOxsources, such as coal combustion and vehicular sources of atmospheric NO3−. There were no significant differences in δ15N of NO3− between east and west sides of the park during summer or winter (p = 0.83), indicating that the two areas may have similar sources of atmospheric NO3−. Results from this study indicate that a combination of IER collectors and snowpack

  15. Seasonal baseline of nutrients and stable isotopes in a saline lake of Argentina: biogeochemical processes and river runoff effects.

    PubMed

    Kopprio, Germán A; Kattner, Gerhard; Freije, R Hugo; de Paggi, Susana José; Lara, Rubén J

    2014-05-01

    The seasonal variability of inorganic and organic nutrients and stable isotopes and their relations with plankton and environmental conditions were monitored in Lake Chasicó. Principal component analysis evidenced the strong influence of the river runoff on several biogeochemical variables. Silicate concentrations were controlled by diatom biomass and river discharge. Higher values of nitrate and soluble reactive phosphorus (SRP) indicated agricultural uses in the river basin. Elevated pH values (∼ 9) inhibiting nitrification in the lake explained partially the dominance of ammonium: ∼ 83 % of dissolved inorganic nitrogen (DIN). The low DIN/SRP ratio inferred nitrogen limitation, although the hypotheses of iron and CO2 limitation are relevant in alkaline lakes. Particulate organic matter (POM) and dissolved organic matter (DOM) were mainly of autochthonous origin. The main allochthonous input was imported by the river as POM owning to the arid conditions. Dissolved organic carbon was likely top-down regulated by the bacterioplankton grazer Brachionus plicatilis. The δ(13)C signature was a good indicator of primary production and its values were influenced probably by CO2 limitation. The δ(15)N did not evidence nitrogen fixation and suggested the effects of anthropogenic activities. The preservation of a good water quality in the lake is crucial for resource management. PMID:24415133

  16. Response of northern San Francisco Bay to riverine inputs of dissolved inorganic carbon, silicon, nitrogen and phosphorus

    USGS Publications Warehouse

    Schemel, Laurence E.; Harmon, Dana D.; Eager, Stephen W.; Peterson, David H.

    1984-01-01

    Estuarine processes can be effective in modifying (filtering) distributions of dissolved inorganic forms of carbon (DIC), silicon (DIS), nitrogen (DIN), and phosphorus (DIP) in northern San Francisco Bay. During winter, high inflow from the Sacramento-San Joaquin river system supplied these nutrients to the estuary at rates that exceeded potential rates of estuarine supply and removal processes. During spring and summer, when inflow rates were lower, the estuary was an effective “filter” of the river inflow “signal” because rates of estuarine processes were high relative to river and other supply rates. At lower inflow rates, the river apparently influenced estuarine hydrodynamic features that controlled rates of phytoplankton nutrient removal. Largest biological removal effects were localized in San Pablo Bay during spring and Suisun Bay during summer, and they were generally more pronounced in shallow water areas of the bays. In San Pablo Bay, effects of biological removal appeared soon after river inflow decreased from high winter rates, but persisted for only a short time. During the following summer months, DIN and DIP distributions in San Pablo Bay indicated that estuarine sources contributed to higher concentrations of these nutrients.

  17. Response of benthic algae to environmental gradients in an agriculturally dominated landscape

    USGS Publications Warehouse

    Munn, M.D.; Black, R.W.; Gruber, S.J.

    2002-01-01

    Benthic algal communities were assessed in an agriculturally dominated landscape in the Central Columbia Plateau, Washington, to determine which environmental variables best explained species distributions, and whether algae species optima models were useful in predicting specific water-quality parameters. Land uses in the study area included forest, range, urban, and agriculture. Most of the streams in this region can be characterized as open-channel systems influenced by intensive dryland (nonirrigated) and irrigated agriculture. Algal communities in forested streams were dominated by blue-green algae, with communities in urban and range streams dominated by diatoms. The predominance of either blue-greens or diatoms in agricultural streams varied greatly depending on the specific site. Canonical correspondence analysis (CCA) indicated a strong gradient effect of several key environmental variables on benthic algal community composition. Conductivity and % agriculture were the dominant explanatory variables when all sites (n = 24) were included in the CCA; water velocity replaced conductivity when the CCA included only agricultural and urban sites. Other significant explanatory variables included dissolved inorganic nitrogen (DIN), orthophosphate (OP), discharge, and precipitation. Regression and calibration models accurately predicted conductivity based on benthic algal communities, with OP having slightly lower predictability. The model for DIN was poor, and therefore may be less useful in this system. Thirty-four algal taxa were identified as potential indicators of conductivity and nutrient conditions, with most indicators being diatoms except for the blue-greens Anabaenasp. and Lyngbya sp.

  18. A Mechanistic Study of Plant and Microbial Controls over R* for Nitrogen in an Annual Grassland

    PubMed Central

    Levine, Jonathan M.; HilleRisLambers, Janneke

    2014-01-01

    Differences in species' abilities to capture resources can drive competitive hierarchies, successional dynamics, community diversity, and invasions. To investigate mechanisms of resource competition within a nitrogen (N) limited California grassland community, we established a manipulative experiment using an R* framework. R* theory holds that better competitors within a N limited community should better depress available N in monoculture plots and obtain higher abundance in mixture plots. We asked whether (1) plant uptake or (2) plant species influences on microbial dynamics were the primary drivers of available soil N levels in this system where N structures plant communities. To disentangle the relative roles of plant uptake and microbially-mediated processes in resource competition, we quantified soil N dynamics as well as N pools in plant and microbial biomass in monoculture plots of 11 native or exotic annual grassland plants over one growing season. We found a negative correlation between plant N content and soil dissolved inorganic nitrogen (DIN, our measure of R*), suggesting that plant uptake drives R*. In contrast, we found no relationship between microbial biomass N or potential net N mineralization and DIN. We conclude that while plant-microbial interactions may have altered the overall quantity of N that plants take up, the relationship between species' abundance and available N in monoculture was largely driven by plant N uptake in this first year of growth. PMID:25170943

  19. Evaluation of a Method Using Three Genomic Guided Escherichia coli Markers for Phylogenetic Typing of E. coli Isolates of Various Genetic Backgrounds.

    PubMed

    Hamamoto, Kouta; Ueda, Shuhei; Yamamoto, Yoshimasa; Hirai, Itaru

    2015-06-01

    Genotyping and characterization of bacterial isolates are essential steps in the identification and control of antibiotic-resistant bacterial infections. Recently, one novel genotyping method using three genomic guided Escherichia coli markers (GIG-EM), dinG, tonB, and dipeptide permease (DPP), was reported. Because GIG-EM has not been fully evaluated using clinical isolates, we assessed this typing method with 72 E. coli collection of reference (ECOR) environmental E. coli reference strains and 63 E. coli isolates of various genetic backgrounds. In this study, we designated 768 bp of dinG, 745 bp of tonB, and 655 bp of DPP target sequences for use in the typing method. Concatenations of the processed marker sequences were used to draw GIG-EM phylogenetic trees. E. coli isolates with identical sequence types as identified by the conventional multilocus sequence typing (MLST) method were localized to the same branch of the GIG-EM phylogenetic tree. Sixteen clinical E. coli isolates were utilized as test isolates without prior characterization by conventional MLST and phylogenetic grouping before GIG-EM typing. Of these, 14 clinical isolates were assigned to a branch including only isolates of a pandemic clone, E. coli B2-ST131-O25b, and these results were confirmed by conventional typing methods. Our results suggested that the GIG-EM typing method and its application to phylogenetic trees might be useful tools for the molecular characterization and determination of the genetic relationships among E. coli isolates. PMID:25809972

  20. Tolerance of Escherichia coli to fluoroquinolone antibiotics depends on specific components of the SOS response pathway.

    PubMed

    Theodore, Alyssa; Lewis, Kim; Vulic, Marin

    2013-12-01

    Bacteria exposed to bactericidal fluoroquinolone (FQ) antibiotics can survive without becoming genetically resistant. Survival of these phenotypically resistant cells, commonly called "persisters," depends on the SOS gene network. We have examined mutants in all known SOS-regulated genes to identify functions essential for tolerance in Escherichia coli. The absence of DinG and UvrD helicases and the Holliday junction processing enzymes RuvA and RuvB leads to a decrease in survival. Analysis of the respective mutants indicates that, in addition to repair of double-strand breaks, tolerance depends on the repair of collapsed replication forks and stalled transcription complexes. Mutation in recF results in increased survival, which identifies RecAF recombination as a poisoning mechanism not previously linked to FQ lethality. DinG acts upstream of SOS promoting its induction, whereas RuvAB participates in repair only. UvrD directly promotes all repair processes initiated by FQ-induced damage and prevents RecAF-dependent misrepair, making it one of the crucial SOS functions required for tolerance. PMID:24077306

  1. Genetic analysis of Escherichia coli RadA: functional motifs and genetic interactions.

    PubMed

    Cooper, Deani L; Boyle, Daniel C; Lovett, Susan T

    2015-03-01

    The RadA/Sms protein is a RecA-related protein found universally in eubacteria and plants, implicated in processing of recombination intermediates. Here we show that the putative Zn finger, Walker A motif, KNRXG motif and Lon protease homology domain of the Escherichia coli RadA protein are required for DNA damage survival. RadA is unlikely to possess protease activity as the putative active site serine is not required. Mutants in RadA have strong synergistic phenotypes with those in the branch migration protein RecG. Sensitivity of radA recG mutants to azidothymidine (AZT) can be rescued by blocking recombination with recA or recF mutations or by overexpression of RuvAB, suggesting that lethal recombination intermediates accumulate in the absence of RadA and RecG. Synthetic genetic interactions for survival to AZT or ciprofloxacin exposure were observed between RadA and known or putative helicases including DinG, Lhr, PriA, Rep, RuvAB, UvrD, YejH and YoaA. These represent the first affected phenotypes reported for Lhr, YejH and YoaA. The specificity of these effects sheds new light on the role of these proteins in DNA damage avoidance and repair and implicates a role in replication gap processing for DinG and YoaA and a role in double-strand break repair for YejH. PMID:25484163

  2. ACID EVAPORATION OF ULTIMA GOLD TM AB LIQUID SCINTILLATION COCKTAIL RESIDUE

    SciTech Connect

    Kyser, E.; Fondeur, F.; Crump, S.

    2011-12-21

    Prior analyses of samples from the F/H Lab solutions showed the presence of diisopropylnapthalene (DIN), a major component of Ultima Gold{trademark} AB liquid scintillation cocktail (LSC). These solutions are processed through H-Canyon Tank 10.5 and ultimately through the 17.8E evaporator. Similar solutions originated in SRNL streams sent to the same H Canyon tanks. This study examined whether the presence of these organics poses a process-significant hazard for the evaporator. Evaporation and calorimetry testing of surrogate samples containing 2000 ppm of Ultima Gold{trademark} AB LSC in 8 M nitric acid have been completed. These experiments showed that although reactions between nitric acid and the organic components do occur, they do not appear to pose a significant hazard for runaway reactions or generation of energetic compounds in canyon evaporators. The amount of off-gas generated was relatively modest and appeared to be well within the venting capacity of the H-Canyon evaporators. A significant fraction of the organic components likely survives the evaporation process primarily as non-volatile components that are not expected to represent any new process concerns during downstream operations such as neutralization. Laboratory Waste solutions containing minor amounts of DIN can be safely received, stored, transferred, and processed through the canyon waste evaporator.

  3. Influence of nutrient input on the trophic state of a tropical brackish water lagoon

    NASA Astrophysics Data System (ADS)

    Ganguly, D.; Patra, Sivaji; Muduli, Pradipta R.; Vardhan, K. Vishnu; R, Abhilash K.; Robin, R. S.; Subramanian, B. R.

    2015-07-01

    Ecosystem level changes in water quality and biotic communities in coastal lagoons have been associated with intensification of anthropogenic pressures. In light of incipient changes in Asia's largest brackish water lagoon (Chilika, India), an examination of different dissolved nutrients distribution and phytoplankton biomass, was conducted through seasonal water quality monitoring in the year 2011. The lagoon showed both spatial and temporal variation in nutrient concentration, mostly altered by freshwater input, regulated the chlorophyll distribution as well. Dissolved inorganic N:P ratio in the lagoon showed nitrogen limitation in May and December, 2011. Chlorophyll in the lagoon varied between 3.38 and 17.66 mg m -3. Spatially, northern part of the lagoon showed higher values of DIN and chlorophyll during most part of the year, except in May, when highest DIN was recorded in the southern part. Statistical analysis revealed that dissolved NH-N and urea could combinedly explain 43% of Chlorophyll- a (Chl- a) variability which was relatively higher than that explained by NO-N and NO-N (12.4%) in lagoon water. Trophic state index calculated for different sectors of the lagoon confirmed the inter-sectoral and inter-seasonal shift from mesotrophic to eutrophic conditions largely depending on nutrient rich freshwater input.

  4. Adaptive mutation and amplification in Escherichia coli: two pathways of genome adaptation under stress.

    PubMed

    Hersh, Megan N; Ponder, Rebecca G; Hastings, P J; Rosenberg, Susan M

    2004-06-01

    The neo-Darwinists suggested that evolution is constant and gradual, and thus that genetic changes that drive evolution should be too. However, more recent understanding of phenomena called adaptive mutation in microbes indicates that mutation rates can be elevated in response to stress, producing beneficial and other mutations. We review evidence that, in Escherichia coli, two separate mechanisms of stress-induced genetic change occur that revert a lac frameshift allele allowing growth on lactose medium. First, compensatory frameshift ("point") mutations occur by a mechanism that includes DNA double-strand breaks and (we have suggested) their error-prone repair. Point mutation requires induction of the RpoS-dependent general stress response, and the SOS DNA damage response leading to upregulation of the error-prone DNA polymerase DinB (Pol IV), and occurs during a transient limitation of post-replicative mismatch repair activity. A second mechanism, adaptive amplification, entails amplification of the leaky lac allele to 20-50 tandem repeats. These provide sufficient beta-galactosidase activity for growth, thereby apparently deflecting cells from the point mutation pathway. Unlike point mutation, amplification neither occurs in hypermutating cells nor requires SOS or DinB, but like point mutation, amplification requires the RpoS-dependent stress response. Similar processes are being found in other bacterial systems and yeast. Stress-induced genetic changes may underlie much of microbial evolution, pathogenesis and antibiotic resistance, and also cancer formation, progression and drug resistance. PMID:15207867

  5. Experimental Behavior of Carbon Fiber Reinforced Isolators

    SciTech Connect

    Russo, Gaetano; Pauletta, Margherita; Cortesia, Andrea; Dal Bianco, Alberto

    2008-07-08

    This paper describes an investigation on the experimental behavior of innovative elastomeric isolators reinforced by carbon fiber fabrics. These fabrics are very much lighter than steel plates used in conventional isolators and able to transfer to the adjacent elastomer layers tangential stresses adequate to oppose the transversal deformation of rubber under vertical loads. The isolators are not bonded to the sub- and super-structure (elimination of the steel end-plates), hence their weight and cost are reduced. The experimental investigation is carried out on small-scale isolator prototypes reinforced by quadridirectional carbon fiber fabrics. The isolators are subjected to the following qualification tests prescribed by the Italian Code 'Ordinanza 3274' for steel reinforced isolators: 1) 'Static assessment of the compression stiffness'; 2) 'Static assessment of the shear modulus G'; 3) 'Dynamic assessment of the dynamic shear modulus G{sub din} and of the damping coefficient {xi}; 4) 'Assessment of the G{sub din}-{gamma} and {xi}-{gamma} diagrams by means of dynamic tests'; 5) 'Assessment of creep characteristics'; 6) 'Evaluation of the capacity of sustaining at least 10 cycles'. As a result of the tests, the isolators survived large shear strains, comparable to those expected for conventional isolators.

  6. Nuclear Quantum Effects in Water at the Triple Point: Using Theory as a Link Between Experiments.

    PubMed

    Cheng, Bingqing; Behler, Jörg; Ceriotti, Michele

    2016-06-16

    One of the most prominent consequences of the quantum nature of light atomic nuclei is that their kinetic energy does not follow a Maxwell-Boltzmann distribution. Deep inelastic neutron scattering (DINS) experiments can measure this effect. Thus, the nuclear quantum kinetic energy can be probed directly in both ordered and disordered samples. However, the relation between the quantum kinetic energy and the atomic environment is a very indirect one, and cross-validation with theoretical modeling is therefore urgently needed. Here, we use state of the art path integral molecular dynamics techniques to compute the kinetic energy of hydrogen and oxygen nuclei in liquid, solid, and gas-phase water close to the triple point, comparing three different interatomic potentials and validating our results against equilibrium isotope fractionation measurements. We will then show how accurate simulations can draw a link between extremely precise fractionation experiments and DINS, therefore establishing a reliable benchmark for future measurements and providing key insights to increase further the accuracy of interatomic potentials for water. PMID:27203358

  7. Direct Measurements of Quantum Kinetic Energy Tensor in Stable and Metastable Water near the Triple Point: An Experimental Benchmark.

    PubMed

    Andreani, Carla; Romanelli, Giovanni; Senesi, Roberto

    2016-06-16

    This study presents the first direct and quantitative measurement of the nuclear momentum distribution anisotropy and the quantum kinetic energy tensor in stable and metastable (supercooled) water near its triple point, using deep inelastic neutron scattering (DINS). From the experimental spectra, accurate line shapes of the hydrogen momentum distributions are derived using an anisotropic Gaussian and a model-independent framework. The experimental results, benchmarked with those obtained for the solid phase, provide the state of the art directional values of the hydrogen mean kinetic energy in metastable water. The determinations of the direction kinetic energies in the supercooled phase, provide accurate and quantitative measurements of these dynamical observables in metastable and stable phases, that is, key insight in the physical mechanisms of the hydrogen quantum state in both disordered and polycrystalline systems. The remarkable findings of this study establish novel insight into further expand the capacity and accuracy of DINS investigations of the nuclear quantum effects in water and represent reference experimental values for theoretical investigations. PMID:27214268

  8. Eutrophication assessment and bioremediation strategy using seaweeds co-cultured with aquatic animals in an enclosed bay in China.

    PubMed

    Wu, Hailong; Huo, Yuanzi; Hu, Ming; Wei, Zhangliang; He, Peimin

    2015-06-15

    Intensive mariculture results in a rise in nutrient concentrations, then leads to serious eutrophication in coastal waters. Based on the sampling data obtained between August 2012 and July 2013, the eutrophication status in Yantian Bay was assessed, and the proportion of marine animals co-cultured with seaweeds was evaluated. The nutritional quality index (NQI) ranged from 4.37 to 13.20, indicating serious eutrophication conditions. The annual average ratio of nitrogen/phosphorus (N/P) was 25.19, indicating a nitrogen surplus in this system. DIN was selected as the best parameter to balance seaweed absorption and marine animal DIN production. Gracilaria lemaneiformis and Laminaria japonica were selected as co-cultured seaweeds. The optimal proportion of G. lemaneiformis production was assessed as 20074.14 tonnes. The optimal proportion of L. japonica production was evaluated as 15890.68 tonnes. High-temperature adapted seaweeds should be introduced for removing nutrients releasing by farmed aquatic animals in the summer in Yantian Bay. PMID:25913794

  9. Abstract and review of "Zur Erbpathologie der Schizophrenie" (Contribution to the genetics of schizophrenia). 1916.

    PubMed

    Kendler, K S; Zerbin-Rüdin, E

    1996-07-26

    Starting from the 722 probands originally studied by Rüdin, Bruno Schulz re-examined them and their relatives confirming the diagnosis in 660. While Rüdin sought for mendelian ratios in siblings, Schulz, anticipating modern methods, focused on the family study method as an approach to clarifying possible etiologic heterogeneity within the schizophrenia syndrome. Using a Kraepelian approach to diagnosis, Schulz reports a MR for narrowly and broadly defined schizophrenia of 6.7 and 8.2% in siblings and 2.6 and 3.7% in parents. He found no evidence for a difference in risk of illness in siblings as a function of either the gender or outcome of the proband. The risk for schizophrenia was significantly increased in siblings of hebephrenic probands. Compared to siblings of probands with no identified factor which precipitated their schizophrenia, the risk for schizophrenia was significantly decreased in probands with a physical etiologic factor but did not differ in siblings of probands with a psychological etiologic factor. The risk for schizophrenia was particularly low in siblings of probands whose onset of illness occurred within a year of major head trauma. PMID:8837700

  10. Biogeochemistry of a treeline watershed, northwestern Alaska

    USGS Publications Warehouse

    Stottlemyer, R.

    2001-01-01

    Since 1950, mean annual temperatures in northwestern Alaska have increased. Change in forest floor and soil temperature or moisture could alter N mineralization rates, production of dissolved organic carbon (DOC) and organic nitrogen (DON), and their export to the aquatic ecosystem. In 1990, we began study of nutrient cycles in the 800-ha Asik watershed, located at treeline in the Noatak National Preserve, northwestern Alaska. This paper summarizes relationships between topographic aspect, soil temperature and moisture, inorganic and organic N pools, C pools, CO2 efflux, growing season net N mineralization rates, and stream water chemistry. Forest floor (O2) C/N ratios, C pools, temperature, and moisture were greater on south aspects. More rapid melt of the soil active layer (zone of annual freeze-thaw) and permafrost accounted for the higher moisture. The O2 C and N content were correlated with moisture, inorganic N pools, CO2 efflux, and inversely with temperature. Inorganic N pools were correlated with temperature and CO2 efflux. Net N mineralization rates were positive in early summer, and correlated with O2 moisture, temperature, and C and N pools. Net nitrification rates were inversely correlated with moisture, total C and N. The CO2 efflux increased with temperature and moisture, and was greater on south aspects. Stream ion concentrations declined and DOC increased with discharge. Stream inorganic nitrogen (DIN) output exceeded input by 70%. Alpine stream water nitrate (NO-3) and DOC concentrations indicated substantial contributions to the watershed DIN and DOC budgets.

  11. Structures of multidrug and toxic compound extrusion transporters and their mechanistic implications.

    PubMed

    Lu, Min

    2016-03-01

    Multidrug resistance poses grand challenges to the effective treatment of infectious diseases and cancers. Integral membrane proteins from the multidrug and toxic compound extrusion (MATE) family contribute to multidrug resistance by exporting a wide variety of therapeutic drugs across cell membranes. MATE proteins are conserved from bacteria to humans and can be categorized into the NorM, DinF and eukaryotic subfamilies. MATE transporters hold great appeal as potential therapeutic targets for curbing multidrug resistance, yet their transport mechanism remains elusive. During the past 5 years, X-ray structures of 4 NorM and DinF transporters have been reported and guided biochemical studies to reveal how MATE transporters extrude different drugs. Such advances, although substantial, have yet to be discussed collectively. Herein I review these structures and the unprecedented mechanistic insights that have been garnered from those structure-inspired studies, as well as lay out the outstanding questions that present exciting opportunities for future work. PMID:26488689

  12. Distribution of typical denitrifying functional genes and diversity of the nirS-encoding bacterial community related to environmental characteristics of river sediments

    NASA Astrophysics Data System (ADS)

    Huang, S.; Chen, C.; Wu, Q.; Zhang, R.; Yang, X.

    2011-05-01

    Denitrification in river sediments leads to nitrate removal from the aquatic system; therefore, it is necessary to understand functional diversity of denitrifier communities in the system. Sediment samples (0-25 cm depth) were collected from three typical locations along the Pearl River. The real-time PCR approach was used to measure the abundance of nitrate (narG), nitrite (nirS, nirK and nrfA), and nitrous oxide (nosZ) reductase genes from the sediment samples. Assemblages of nirS, nirK and nosZ indicated that complete denitrification occurred in sediment cores, with the greatest number of gene copies from 5-15 cm depth. Dissimilatory nitrate reduction appeared to be important below 15 cm depth, based on increasing gene copies of narG and nrfA with sediment depth. There was a close match (78-94 %) between the nirS sequences recovered from Pearl River sediment and those detected in estuarine and marine sediments as well as active sludge, suggesting that domestic sewage inputs and irregular tides. Canonical correspondence analysis indicated that the spatial distribution of denitrifying bacteria was highly correlated with dissolved inorganic N (DIN: NH4+, NO2DIN within the sediment profile influences the distribution of denitrifying genes and the nirS-encoding denitrifier community in the river sediment. Our results also reveal a variety of novel denitrifying bacteria in the river sediment.

  13. Crustal deformation associated with an M8.1 earthquake in the Solomon Islands, detected by ALOS/PALSAR

    NASA Astrophysics Data System (ADS)

    Miyagi, Yousuke; Ozawa, Taku; Shimada, Masanobu

    2009-10-01

    On April 1, 2007 (UTC), a large Mw 8.1 interplate earthquake struck the Solomon Islands subduction zone where complicated tectonics result from the subduction of four plates. Extensive ground movements and a large tsunami occurred in the epicentral area causing severe damage over a wide area. Using ALOS/PALSAR data and the DInSAR technique, we detected crustal deformation exceeding 2 m in islands close to the epicenter. A slip distribution of the inferred seismic fault was estimated using geodetic information derived from DInSAR processing and field investigations. The result indicates large slip areas around the hypocenter and the centroid. It is possible that the largest slip area is related to subduction of the plate boundary between the Woodlark and Australian plates. A small slip area between those large slip areas may indicate weak coupling due to thermal activity related to volcanic activity on Simbo Island. The 2007 earthquake struck an area where large earthquake has not occurred since 1970. Most of this seismic gap was filled by the 2007 events, however a small seismic gap still remains in the southeastern region of the 2007 earthquake.

  14. Gravity-driven deformation of Tenerife measured by InSAR time series analysis

    NASA Astrophysics Data System (ADS)

    Fernández, J.; Tizzani, P.; Manzo, M.; Borgia, A.; González, P. J.; Martí, J.; Pepe, A.; Camacho, A. G.; Casu, F.; Berardino, P.; Prieto, J. F.; Lanari, R.

    2009-02-01

    We study the state of deformation of Tenerife (Canary Islands) using Differential Synthetic Aperture Radar Interferometry (DInSAR). We apply the Small BAseline Subset (SBAS) DInSAR algorithm to radar images acquired from 1992 to 2005 by the ERS sensors to determine the deformation rate distribution and the time series for the coherent pixels identified in the island. Our analysis reveals that the summit area of the volcanic edifice is characterized by a rather continuous subsidence extending well beyond Las Cañadas caldera rim and corresponding to the dense core of the island. These results, coupled with GPS ones, structural and geological information and deformation modeling, suggest an interpretation based on the gravitational sinking of the dense core of the island into a weak lithosphere and that the volcanic edifice is in a state of compression. We also detect more localized deformation patterns correlated with water table changes and variations in the deformation time series associated with the seismic crisis in 2004.

  15. Development and pilot evaluation of a cancer-focused summer research education program for [corrected] Navajo undergraduate students.

    PubMed

    Garrison, Edward R; Bauer, Mark C; Hosley, Brenda L; Patten, Christi A; Hughes, Christine A; Trapp, Mary A; Petersen, Wesley O; Austin-Garrison, Martha A; Bowman, Clarissa N; Vierkant, Robert A

    2010-12-01

    This paper describes the development and pilot testing of a 10-week cancer research education program for Navajo undergraduate students. The program was piloted at Diné College with 22 undergraduates (7 men, 15 women) in 2007 and 2008. Students completed a pre-post program survey assessing attitudes, opinions, and knowledge about research and about cancer. The program was found to be culturally acceptable and resulted in statistically significant changes in some of the attitudes and opinions about research and cancer. Combining all 13 knowledge items, there was a significant (p = 0.002) change in the mean total correct percent from baseline [70.3 (SD = 15.9)] to post-program [82.1 (SD = 13.1)]. The curriculum was adapted for a new cancer prevention and control course now offered at Diné College, enhancing sustainability. Ultimately, these efforts may serve to build capacity in communities by developing a cadre of future Native American scientists to develop and implement cancer research. PMID:20411446

  16. Relationship between environmental factors and plankton in the Bayuquan Port, Liaodong Bay, China: a five-year study

    NASA Astrophysics Data System (ADS)

    Song, Lun; Yang, Guojun; Wang, Nianbin; Lu, Xiaoqian

    2015-11-01

    To understand the relationship between the plankton community and environmental factors and water quality in the Bayuquan Port of Liaodong Bay, China, and investigations were carried out during six different periods (April 2009, April 2010, October 2011, April 2012, October 2012, and April 2013). This area was characterized by high levels of nutrient and suspended solids (SPS) during survey periods, and eutrophication led to the occurrence of red tides in April and October 2012 and April 2013. Our analyses revealed that the plankton communities of Bayuquan Port lacked stability and were affected seriously by external disturbance (e.g., oceanographic engineering and river runoff). Our data indicate that oil, dissolved inorganic nitrogen (DIN), SPS, and chlorophyll a (Chl-a) were key factors that regulated the phytoplankton and zooplankton communities. The partial redundancy analysis (partial RDA) suggested that oil and SPS were the most important environmental variables affecting the phytoplankton community in April 2010 and 2012, whereas DIN concentration played a governing role in zooplankton dynamics. Oil and Chl- a concentrations affected significantly the zooplankton community in October 2012. Therefore, the plankton communities could reflect both dynamic changes in coastal environmental factors and the ongoing eutrophication process caused by anthropogenic activities in this area.

  17. Dissolved inorganic nitrogen composition, transformation, retention, and transport in naturally phosphate-rich and phosphate-poor tropical streams

    USGS Publications Warehouse

    Triska, F.J.; Pringle, C.M.; Zellweger, G.W.; Duff, J.H.; Avanzino, R.J.

    1993-01-01

    In Costa Rica, the Salto River is enriched by geothermal-based soluble reactive phosphorus (SRP), which raises the concentration up to 200 ??g/L whereas Pantano Creek, an unimpacted tributary, has an SRP concentration <10 ??g/L. Ammonium concentration in springs adjacent to the Salto and Pantano was typically greater than channel water (13 of 22 locations) whereas nitrate concentration was less (20 of 22 locations). Ground waters were typically high in ammonium relative to nitrate whereas channel waters were high in nitrate relative to ammonium. Sediment slurry studies indicated nitrification potential in two sediment types, firm clay (3.34 ??g N.cm-3.d-1) and uncompacted organic-rich sediment (1.76 ??g N.cm-3.d-1). Ammonium and nitrate amendments to each stream separately resulted in nitrate concentrations in excess of that expected after correlation for dilution using a conservative tracer. SRP concentration was not affected by DIN amendment to either stream. SRP concentration in the Pantano appeared to be regulated by abiotic sediment exchange reactions. DIN composition and concentration were regulated by a combination of biotic and abiotic processes. -from Authors

  18. Structures of multidrug and toxic compound extrusion transporters and their mechanistic implications

    PubMed Central

    Lu, Min

    2016-01-01

    ABSTRACT Multidrug resistance poses grand challenges to the effective treatment of infectious diseases and cancers. Integral membrane proteins from the multidrug and toxic compound extrusion (MATE) family contribute to multidrug resistance by exporting a wide variety of therapeutic drugs across cell membranes. MATE proteins are conserved from bacteria to humans and can be categorized into the NorM, DinF and eukaryotic subfamilies. MATE transporters hold great appeal as potential therapeutic targets for curbing multidrug resistance, yet their transport mechanism remains elusive. During the past 5 years, X-ray structures of 4 NorM and DinF transporters have been reported and guided biochemical studies to reveal how MATE transporters extrude different drugs. Such advances, although substantial, have yet to be discussed collectively. Herein I review these structures and the unprecedented mechanistic insights that have been garnered from those structure-inspired studies, as well as lay out the outstanding questions that present exciting opportunities for future work. PMID:26488689

  19. Computation and visualization of the MacAdam limits for any lightness, hue angle, and light source

    NASA Astrophysics Data System (ADS)

    Martínez-Verdú, Francisco; Perales, Esther; Chorro, Elisabet; de Fez, Dolores; Viqueira, Valentín; Gilabert, Eduardo

    2007-06-01

    We present a systematic algorithm capable of searching for optimal colors for any lightness L* (between 0 and 100), any illuminant (D65, F2, F7, F11, etc.), and any light source reported by CIE. Color solids are graphed in some color spaces (CIELAB, SVF, DIN99d, and CIECAM02) by horizontal (constant lightness) and transversal (constant hue angle) sections. Color solids plotted in DIN99d and CIECAM02 color spaces look more spherical or homogeneous than the ones plotted in CIELAB and SVF color spaces. Depending on the spectrum of the light source or illuminant, the shape of its color solid and its content (variety of distinguishable colors, with or without color correspondence) change drastically, particularly with sources whose spectrum is discontinuous and/or very peaked, with correlated color temperature lower than 5500 K. This could be used to propose an absolute colorimetric quality index for light sources comparing the volumes of their gamuts, in a uniform color space.

  20. Modeling leaching behavior of solidified wastes using back-propagation neural networks.

    PubMed

    Bayar, Senem; Demir, Ibrahim; Engin, Guleda Onkal

    2009-03-01

    In a previous study, treatment sludge obtained from a chemical industry, which contained potentially toxic heavy metals and organics, was characterized and solidified by solidification/stabilization (S/S). In this study, however, the prediction of leaching behavior of the sludge by linear regression method and neural networks (NNs) was discussed. NN analysis was used to construct models of leaching behavior as a function of mix composition (waste/binder ratio, W/B) using existing data from the previous study of cement-based S/S. The differences in leaching rate of each metal were also considered. The hazard characteristics of the waste were determined as defined in both Turkish and US EPA regulations, by means of Extraction Procedure Toxicity Test (EPTox) and DIN 38414-S4 Test. S/S studies were conducted using Portland cement to solidify the sludge containing high amount of Cr, Cu, Hg, Ni, Pb, and Zn. The W/B ratios of 36 specimens were kept between 0/100 and 40/100. The specimens were cured at room temperature for 7, 28, and 90 days. The heavy metal content of the extracts of each specimen was detected usually less than standard concentrations in EPTox and DIN 38414-S4 leaching procedures. By the use of NN, leaching behavior of the solidified wastes can be predicted and, therefore, optimum S/S technologies can be achieved. PMID:18068228

  1. Ernst Rüdin’s Unpublished 1922-1925 Study “Inheritance of Manic-Depressive Insanity”: Genetic Research Findings Subordinated to Eugenic Ideology

    PubMed Central

    Kösters, Gundula; Steinberg, Holger; Kirkby, Kenneth Clifford; Himmerich, Hubertus

    2015-01-01

    In the early 20th century, there were few therapeutic options for mental illness and asylum numbers were rising. This pessimistic outlook favoured the rise of the eugenics movement. Heredity was assumed to be the principal cause of mental illness. Politicians, scientists and clinicians in North America and Europe called for compulsory sterilisation of the mentally ill. Psychiatric genetic research aimed to prove a Mendelian mode of inheritance as a scientific justification for these measures. Ernst Rüdin’s seminal 1916 epidemiological study on inheritance of dementia praecox featured large, systematically ascertained samples and statistical analyses. Rüdin’s 1922–1925 study on the inheritance of “manic-depressive insanity” was completed in manuscript form, but never published. It failed to prove a pattern of Mendelian inheritance, counter to the tenets of eugenics of which Rüdin was a prominent proponent. It appears he withheld the study from publication, unable to reconcile this contradiction, thus subordinating his carefully derived scientific findings to his ideological preoccupations. Instead, Rüdin continued to promote prevention of assumed hereditary mental illnesses by prohibition of marriage or sterilisation and was influential in the introduction by the National Socialist regime of the 1933 “Law for the Prevention of Hereditarily Diseased Offspring” (Gesetz zur Verhütung erbkranken Nachwuchses). PMID:26544949

  2. Contribution of Outer-shelf Deep Water to the nutrient inventories in the euphotic zone of Changjiang River plume during summer

    NASA Astrophysics Data System (ADS)

    Chen, Jianfang

    2016-04-01

    Nutrient supply to the euphotic zone of high productivity area in the Changjiang Estuary,East China Sea is crucial in understanding algal bloom and hypoxia in this area. Previous studies suggested that Changjiang Diluted Water (CDW) and Outer-shelf Deep Water (ODW) are two main sources for nutrient supply while quantitative estimation of each nutrient species to the euphotic zone remains unknown. Based on a field survey and end-member mixing model, the nutrient inventories of ODW, and its contribution to the model-predicted nutrient inventory in the euphotic zone of Changjiang River plume-impacted area were assessed. Upwelling of ODW was identified, indicating that it significantly contributed to nutrient inventories in the upper layer. The nutrient inventory estimation suggested that the area-integrated nutrient inventories of ODW were higher than that of CDW in the euphotic zone of plume-impacted area. As inferred from the mixing model, the contributions of ODW to DIN and silicate inventories in the euphotic zone were 18.2% and 28.1%, respectively, much lower than that of CDW. However, 37.6% and 39.1% of the phosphate inventory were from ODW and CDW, respectively, indicating that ODW phosphate contribution was comparable with that of CDW in the euphotic zone, while DIN and silicate brought by ODW were less

  3. The Y-Family DNA Polymerase Dpo4 Uses a Template Slippage Mechanism To Create Single-Base Deletions

    SciTech Connect

    Y Wu; R Wilson; J Pata

    2011-12-31

    The Y-family polymerases help cells tolerate DNA damage by performing translesion synthesis, yet they also can be highly error prone. One distinctive feature of the DinB class of Y-family polymerases is that they make single-base deletion errors at high frequencies in repetitive sequences, especially those that contain two or more identical pyrimidines with a 5? flanking guanosine. Intriguingly, different deletion mechanisms have been proposed, even for two archaeal DinB polymerases that share 54% sequence identity and originate from two strains of Sulfolobus. To reconcile these apparent differences, we have characterized Dpo4 from Sulfolobus solfataricus using the same biochemical and crystallographic approaches that we have used previously to characterize Dbh from Sulfolobus acidocaldarius. In contrast to previous suggestions that Dpo4 uses a deoxynucleoside triphosphate (dNTP)-stabilized misalignment mechanism when creating single-base deletions, we find that Dpo4 predominantly uses a template slippage deletion mechanism when replicating repetitive DNA sequences, as was previously shown for Dbh. Dpo4 stabilizes the skipped template base in an extrahelical conformation between the polymerase and the little-finger domains of the enzyme. This contrasts with Dbh, in which the extrahelical base is stabilized against the surface of the little-finger domain alone. Thus, despite sharing a common deletion mechanism, these closely related polymerases use different contacts with the substrate to accomplish the same result.

  4. Environmental factors affecting distribution and abundance of bacteria, fungi and protozoa in subsurface sediments of the Upper Atlantic Coastal Plain, USA

    SciTech Connect

    Levine, S.N.; Ghiorse, W.C.

    1990-12-31

    Exploratory statistical analyses of microbiological, hydrological and geochemical data for samples from four boreholes drilled into Upper Atlantic Coastal Plain sediments near the Savannah River Site, SC, showed highly significant correlations between bacterial abundance (AODC and CFU) and hydraulic conductivity (K). Sediment texture variables (% sand (S), % silt, % clay (C), and S/C) were strongly interrelated with K and, therefore, also correlated with bacterial abundance. AODC did not correlate with the concentrations of dissolved inorganic nitrogen (DIN) or dissolved organic carbon (DOC) in pore water. CFU also did not correlate with DIN, but a negative relationship was found between the CFU and DOC for sandy sediments, suggesting that microbial activity may control pore water DOC concentration. In some, but not all boreholes, AODC and CFU correlated negatively with pore water concentrations of metals and positively with pH. Protozoan abundance correlated strongly with AODC and CFU in the two boreholes closest to the recharge areas for their major aquifers. It also correlated with sediment texture variables, but not with K. Fungal abundance did not correlate with the abundance of other microbial types when data from individual boreholes were considered; however it did correlate with both bacterial and protozoan abundance when data from all four boreholes were combined. There was no relationship between fungal abundance and either K or sediment texture.

  5. Environmental factors affecting distribution and abundance of bacteria, fungi and protozoa in subsurface sediments of the Upper Atlantic Coastal Plain, USA

    SciTech Connect

    Levine, S.N.; Ghiorse, W.C.

    1990-01-01

    Exploratory statistical analyses of microbiological, hydrological and geochemical data for samples from four boreholes drilled into Upper Atlantic Coastal Plain sediments near the Savannah River Site, SC, showed highly significant correlations between bacterial abundance (AODC and CFU) and hydraulic conductivity (K). Sediment texture variables (% sand (S), % silt, % clay (C), and S/C) were strongly interrelated with K and, therefore, also correlated with bacterial abundance. AODC did not correlate with the concentrations of dissolved inorganic nitrogen (DIN) or dissolved organic carbon (DOC) in pore water. CFU also did not correlate with DIN, but a negative relationship was found between the CFU and DOC for sandy sediments, suggesting that microbial activity may control pore water DOC concentration. In some, but not all boreholes, AODC and CFU correlated negatively with pore water concentrations of metals and positively with pH. Protozoan abundance correlated strongly with AODC and CFU in the two boreholes closest to the recharge areas for their major aquifers. It also correlated with sediment texture variables, but not with K. Fungal abundance did not correlate with the abundance of other microbial types when data from individual boreholes were considered; however it did correlate with both bacterial and protozoan abundance when data from all four boreholes were combined. There was no relationship between fungal abundance and either K or sediment texture.

  6. The National Institutes of Health Clinical Center Digital Imaging Network, Picture Archival and Communication System, and Radiology Information System.

    PubMed

    Goldszal, A F; Brown, G K; McDonald, H J; Vucich, J J; Staab, E V

    2001-06-01

    In this work, we describe the digital imaging network (DIN), picture archival and communication system (PACS), and radiology information system (RIS) currently being implemented at the Clinical Center, National Institutes of Health (NIH). These systems are presently in clinical operation. The DIN is a redundant meshed network designed to address gigabit density and expected high bandwidth requirements for image transfer and server aggregation. The PACS projected workload is 5.0 TB of new imaging data per year. Its architecture consists of a central, high-throughput Digital Imaging and Communications in Medicine (DICOM) data repository and distributed redundant array of inexpensive disks (RAID) servers employing fiber-channel technology for immediate delivery of imaging data. On demand distribution of images and reports to clinicians and researchers is accomplished via a clustered web server. The RIS follows a client-server model and provides tools to order exams, schedule resources, retrieve and review results, and generate management reports. The RIS-hospital information system (HIS) interfaces include admissions, discharges, and transfers (ATDs)/demographics, orders, appointment notifications, doctors update, and results. PMID:11442088

  7. Development and Pilot Evaluation of a Cancer-Focused Summer Research Education Program for Navajo Undergraduate Students

    PubMed Central

    Garrison, Edward R.; Bauer, Mark C.; Hosley, Brenda L.; Patten, Christi A.; Hughes, Christine A.; Trapp, Mary A.; Petersen, Wesley O.; Austin-Garrison, Martha A.; Bowman, Clarissa N.; Vierkant, Robert A.

    2010-01-01

    This paper describes the development and pilot testing of a 10-week cancer research education program for Navajo undergraduate students. The program was piloted at Diné College with 22 undergraduates (7 men, 15 women) in 2007 and 2008. Students completed a pre–post program survey assessing attitudes, opinions, and knowledge about research and about cancer. The program was found to be culturally acceptable and resulted in statistically significant changes in some of the attitudes and opinions about research and cancer. Combining all 13 knowledge items, there was a significant (p=0.002) change in the mean total correct percent from baseline [70.3 (SD=15.9)] to post-program [82.1 (SD=13.1)]. The curriculum was adapted for a new cancer prevention and control course now offered at Diné College, enhancing sustainability. Ultimately, these efforts may serve to build capacity in communities by developing a cadre of future Native American scientists to develop and implement cancer research. PMID:20411446

  8. Land movement monitoring at the Mavropigi lignite mine using spaceborne D-InSAR

    NASA Astrophysics Data System (ADS)

    Papadaki, Eirini; Tripolitsiotis, Achilleas; Steiakakis, Chrysanthos; Agioutantis, Zacharias; Mertikas, Stelios; Partsinevelos, Panagiotis; Schilizzi, Pavlos

    2013-08-01

    This paper examines the capability of remote sensing radar interferometry to monitor land movements, as it varies with time, in areas close to open pit lignite mines. The study area is the "Mavropigi" lignite mine in Ptolemais, Northern Greece; whose continuous operation is of vital importance to the electric power supply of Greece. The mine is presently 100-120m deep while horizontal and vertical movements have been measured in the vicinity of the pit. Within the mine, ground geodetic monitoring has revealed an average rate of movement amounting to 10-20mm/day at the southeast slopes. In this work, differential interferometry (DInSAR), using 19 Synthetic Aperture Radar (SAR) images of ALOS satellite, has been applied to monitor progression of land movement caused my mining within the greater area of "Mavropigi" region. The results of this work show that DInSAR can be used effectively to capture ground movement information, well before signs of movements can be observed visually in the form of imminent fissures and tension cracks. The advantage of remote sensing interferometry is that it can be applied even in inaccessible areas where monitoring with ground equipment is either impossible or of high-cost (large areas).

  9. Relationship between environmental factors and plankton in the Bayuquan Port, Liaodong Bay, China: a five-year study

    NASA Astrophysics Data System (ADS)

    Song, Lun; Yang, Guojun; Wang, Nianbin; Lu, Xiaoqian

    2016-07-01

    To understand the relationship between the plankton community and environmental factors and water quality in the Bayuquan Port of Liaodong Bay, China, and investigations were carried out during six different periods (April 2009, April 2010, October 2011, April 2012, October 2012, and April 2013). This area was characterized by high levels of nutrient and suspended solids (SPS) during survey periods, and eutrophication led to the occurrence of red tides in April and October 2012 and April 2013. Our analyses revealed that the plankton communities of Bayuquan Port lacked stability and were affected seriously by external disturbance (e.g., oceanographic engineering and river runoff). Our data indicate that oil, dissolved inorganic nitrogen (DIN), SPS, and chlorophyll a (Chl- a) were key factors that regulated the phytoplankton and zooplankton communities. The partial redundancy analysis (partial RDA) suggested that oil and SPS were the most important environmental variables affecting the phytoplankton community in April 2010 and 2012, whereas DIN concentration played a governing role in zooplankton dynamics. Oil and Chl- a concentrations affected significantly the zooplankton community in October 2012. Therefore, the plankton communities could reflect both dynamic changes in coastal environmental factors and the ongoing eutrophication process caused by anthropogenic activities in this area.

  10. An SOS-regulated operon involved in damage-inducible mutagenesis in Caulobacter crescentus

    PubMed Central

    Galhardo, Rodrigo S.; Rocha, Raquel P.; Marques, Marilis V.; Menck, Carlos F. M.

    2005-01-01

    DNA polymerases of the Y-family, such as Escherichia coli UmuC and DinB, are specialized enzymes induced by the SOS response, which bypass lesions allowing the continuation of DNA replication. umuDC orthologs are absent in Caulobacter crescentus and other bacteria, raising the question about the existence of SOS mutagenesis in these organisms. Here, we report that the C.crescentus dinB ortholog is not involved in damage-induced mutagenesis. However, an operon composed of two hypothetical genes and dnaE2, encoding a second copy of the catalytic subunit of Pol III, is damage inducible in a recA-dependent manner, and is responsible for most ultraviolet (UV) and mitomycin C-induced mutations in C.crescentus. The results demonstrate that the three genes are required for the error-prone processing of DNA lesions. The two hypothetical genes were named imuA and imuB, after inducible mutagenesis. ImuB is similar to proteins of the Y-family of polymerases, and possibly cooperates with DnaE2 in lesion bypass. The mutations arising as a consequence of the activity of the imuAB dnaE2 operon are rather unusual for UV irradiation, including G:C to C:G transversions. PMID:15886391

  11. Dispersión de velocidades en cúmulos de galaxias

    NASA Astrophysics Data System (ADS)

    Muriel, H.; García Lambas, D.; Quintana, H.; Infante, L.

    Se ha estudiado la dinámica de galaxias en cúmulos en base a una muestra de aproximadamente 4000 galaxias con estimas de velocidad radial pertenecientes a 40 cúmulos. Se desarrolló un algoritmo para identificar sub-estructuras del tipo ``grupos de galaxias". Una vez eliminada este tipo de sub-estructura se computaron los perfiles radiales de dispersión de velocidades (VDP) para los 40 cúmulos de galaxias. Dada las características de la muestra, fue posible estimar el VDP hasta grandes distancias del centro del cúmulo llegando en algunos casos hasta 7 Mpc-1. Los resultados indican que el 65% de los cúmulos presentan un VDP plano a grandes radios consistente con el modelo isotermo. Estos resultados son discutidos en el marco de las recientes controversias suscitadas respecto de la dinámica de los cúmulos vía los perfiles de temperaturas de cúmulos derivados de la emisión en rayos-X.

  12. Chemical composition and biological activity of essential oil from Pulicaria undulata from Yemen.

    PubMed

    Ali, Nasser A Awadh; Sharopov, Farukh S; Alhaj, Mehdi; Hill, Gabrielle M; Porzel, Andrea; Arnold, Norbert; Setzer, William N; Schmidt, Jürgen; Wessjohann, Ludger

    2012-02-01

    The chemical composition of the essential oil obtained from the leaves of Pulicaria undulata Gamal Ed Din (syn P. orientalis sensu Schwartz and P. jaubertii Gamal Ed Din) was analyzed by GC-MS. Major compounds of P. undulata oil were the oxygenated monoterpenenes, carvotanacetone (91.4%) and 2,5-dimethoxy-p-cymene (2.6.%). The antimicrobial activity of the essential oil was evaluated against six microorganisms, Escherichia coli Pseudomonas aeruginosa, Staphylococcus aureus, methicillin-resistant S. aureus, Bacillus subtilis, and Candida albicans, using disc diffusion and broth microdilution methods. The oil showed the strongest bactericidal activity against Staphylococcus aureus and methicillin-resistant S. aureus, as well as Candida albicans. The essential oil showed moderate cytotoxic activity against MCF-7 breast tumor cells, with an IC50 of 64.6 +/- 13.7 microg/mL. Bioautographic assays were used to evaluate the acetylcholinesterase inhibitory effect as well as antifungal activity of the oil against Cladosporium cucumerinum. PMID:22474974

  13. Microbiological irrigation water quality of the Marchfeld canal system.

    PubMed

    Stauffer, F; Makristathis, A; Hassl, A; Nowotny, S; Neudorfer, W

    2001-07-01

    The Marchfeld basin with a size of approximately 1000 km2 represents the Austrian "granary". To prevent shortage of water as a result of increased ground water removal for irrigation, industrial purposes and drinking water supply, a canal being 18 km in length was constructed from the Danube to the center of the Marchfeld. From there, water is further distributed via two creeks (Russbach and Stempfelbach). This study was intended to evaluate whether the surface water of the Marchfeld canal system can be classified into hygienic-microbiological categories as proposed by DIN (Deutsche Industrienorm) standards for irrigation water. For this purpose, water sampled monthly from three different sampling sites from 1996 to 1999 was examined for E. coli and enterococci. In addition, water samples were examined for salmonella twice a year from 1996 to 1998 and for cryptosporidia six times during the year 1999. Though the water showed varying degrees of fecal load, the results of the examinations revealed that only one of the three sampling sites showed constant quality levels according to the DIN classification system over prolonged periods of time. However, exceeding of the limit values was occasionally observed indicating the need for regular bacteriological examinations. The high variation of the results from the other sampling sites hardly permits a definite classification in one of the quality classes. PMID:11556148

  14. Spatio-temporal evolution of aseismic ground deformation in the Mexicali Valley (Baja California, Mexico) from 1993 to 2010, using differential SAR interferometry

    NASA Astrophysics Data System (ADS)

    Sarychikhina, O.; Glowacka, E.

    2015-11-01

    Ground deformation in Mexicali Valley, Baja California, Mexico, the southern part of the Mexicali-Imperial valley, is influenced by active tectonics and human activity, mainly that of geothermal fluid extraction in the Cerro Prieto Geothermal Field. Significant ground deformation, mainly subsidence (~ 18 cm yr-1), and related ground fissures cause severe damage to local infrastructure. The technique of Differential Synthetic Aperture Radar Interferometry (DInSAR) has been demonstrated to be a very effective remote sensing tool for accurately measuring the spatial and temporal evolution of ground displacements over broad areas. In present study ERS-1/2 SAR and ENVISAT ASAR images acquired between 1993 and 2010 were used to perform a historical analysis of aseismic ground deformation in Mexicali Valley, in an attempt to evaluate its spatio-temporal evolution and improve the understanding of its dynamic. For this purpose, the conventional 2-pass DInSAR was used to generate interferograms which were used in stacking procedure to produce maps of annual aseismic ground deformation rates for different periods. Differential interferograms that included strong co-seismic deformation signals were not included in the stacking and analysis. The changes in the ground deformation pattern and rate were identified. The main changes occur between 2000 and 2005 and include increasing deformation rate in the recharge zone and decreasing deformation rate in the western part of the CPGF production zone. We suggested that these changes are mainly caused by production development in the Cerro Prieto Geothermal Field.

  15. Six years of land subsidence in shanghai revealed by JERS-1 SAR data

    USGS Publications Warehouse

    Damoah-Afari, P.; Ding, X.-L.; Li, Z.; Lu, Zhiming; Omura, M.

    2008-01-01

    Differential interferometric synthetic aperture radar (SAR) (DInSAR) has proven to be very useful in mapping and monitoring land subsidence in many regions of the world. Shanghai, China's largest city, is one of such areas suffering from land subsidence as a result of severe withdrawal of groundwater for different usages. DInSAR application in Shanghai with the C-band European Remote Sensing 1 & 2 (ERS-1/2) SAR data has been difficult mainly due to the problem of decorrelation of InSAR pairs with temporal baselines larger than 10 months. To overcome the coherence loss of C-band InSAR data, we used eight L-band Japanese Earth Resource Satellite (JERS-1) SAR data acquired during 2 October 1992 to 15 July 1998 to study land subsidence phenomenon in Shanghai. Three of the images were used to produce two separate digital elevation models (DEMs) of the study area to remove topographic fringes from the interferograms used for subsidence mapping. Six interferograms were used to generate 2 different time series of deformation maps over Shanghai. The cumulative subsidence map generated from each of the time series is in agreement with the land subsidence measurements of Shanghai city from 1990-1998, produced from other survey methods. ?? 2007 IEEE.

  16. Eliminating "ductal carcinoma in situ" and "lobular carcinoma in situ" (DCIS and LCIS) terminology in clinical breast practice: The cognitive psychology point of view.

    PubMed

    Pravettoni, Gabriella; Yoder, Whitney R; Riva, Silvia; Mazzocco, Ketti; Arnaboldi, Paola; Galimberti, Viviana

    2016-02-01

    There is evidence from the literature that the terms "ductal carcinoma in situ" and "lobular carcinoma in situ" (DCIS and LCIS) should be eliminated in clinical breast cancer practice and replaced with the new "ductal intraepithelial neoplasia" (DIN) and "lobular intraepithelial neoplasia" (LIN) terminology. The main purpose of the present article is to expand on this argument from a cognitive psychology perspective and offer suggestions for further research, emphasizing how the elimination of the term "carcinoma" in "in situ" breast cancer diagnoses has the potential to reduce both patient and health care professional confusion and misperceptions that are often associated with the DCIS and LCIS diagnoses, as well as limit the adverse psychological effects of women receiving a DCIS or LCIS diagnosis. We comment on the recent peer-reviewed literature on the clinical implications and psychological consequences for breast cancer patients receiving a DCIS or LCIS diagnosis and we use a cognitive perspective to offer new insight into the benefits of embracing the new DIN and LIN terminology. Using cognitive psychology and cognitive science in general, as a foundation, further research is advocated in order to yield data in support of changing the terminology and therefore, offer a chance to significantly improve the lives and psychological sequelae of women facing such a diagnosis. Typology: Controversies/Short Commentary. PMID:26614547

  17. Multislice simulation of transmission electron microscopy imaging of helium bubbles in Fe.

    PubMed

    Yao, Bo; Edwards, Danny J; Kurtz, Richard J; Odette, G Robert; Yamamoto, Takuya

    2012-01-01

    Formation of nanoscale helium (He) bubbles in reduced activation ferritic/martensitic steels may lead to degradation of mechanical properties of materials. Transmission electron microscopy (TEM) has commonly been used to image the Fresnel contrast of He bubbles, using an underfocus of 0.5-1 µm. This paper presents our study of multislice simulation of the size correlation between imaged Fresnel rings and the actual He bubbles. It was found that for bubbles equal to or >3 nm in diameter, the imaged bubble size, represented by its inner diameter of the first dark Fresnel ring (D(in)) in underfocused imaging conditions, increases with increasing electron-beam incoherency, but decreases with increasing underfocus. The electron-beam accelerating voltage, bubble size, bubble position and TEM sample thickness were found to have no significant influence on the deviation of D(in) from the actual bubble size (D(0)). However, for bubbles equal to or <2 nm, D(in)/D(0) increases dramatically with increasing underfocus when it is above a threshold limit (e.g. Δf = -1 µm for a 2-nm bubble). The results of this study also suggested that He bubbles can be differentiated from argon (Ar) bubbles by contrast differences. PMID:23042825

  18. [Defective function of bioindicators for steam sterilization].

    PubMed

    Botzenhart, K; Merkt-Kinzler, M

    1990-05-01

    It can be shown, that under certain conditions commercially available indicators with Bacillus stearothermophilus and packages of native spores from soil prepared according to DIN 58 946/4 react differently to treatment in a lab-type steam sterilizer. The differences were most evident when incomplete evacuation of air had to be supposed. These results lead to the conclusion that some bioindicators are not able to show the inefficient function of steam sterilizers caused by local residuals of air. This may be caused by the properties of the selected strain, by the conditions of growth and preparation of the spores and by the culture medium used after exposition. The results of our experiments as well as the resistance of mesophilic spore forming bacilli against dry heat described by other authors make it necessary to test the resistance of bioindicators for steam sterilization not only against steam according to DIN 58946/4 but also against dry heat or mixtures of steam and air. PMID:2393488

  19. A Faculty Development Program can result in an improvement of the quality and output in medical education, basic sciences and clinical research and patient care.

    PubMed

    Dieter, Peter Erich

    2009-07-01

    The Carl Gustav Carus Faculty of Medicine, University of Technology Dresden, Germany, was founded in 1993 after the reunification of Germany. In 1999, a reform process of medical education was started together with Harvard Medical International.The traditional teacher- and discipline-centred curriculum was displaced by a student-centred, interdisciplinary and integrative curriculum, which has been named Dresden Integrative Patient/Problem-Oriented Learning (DIPOL). The reform process was accompanied and supported by a parallel-ongoing Faculty Development Program. In 2004, a Quality Management Program in medical education was implemented, and in 2005 medical education received DIN EN ISO 9001:2000 certification. Quality Management Program and DIN EN ISO 9001:2000 certification were/are unique for the 34 medical schools in Germany.The students play a very important strategic role in all processes. They are members in all committees like the Faculty Board, the Board of Study Affairs (with equal representation) and the ongoing audits in the Quality Management Program. The Faculty Development program, including a reform in medical education, the establishment of the Quality Management program and the certification, resulted in an improvement of the quality and output of medical education and was accompanied in an improvement of the quality and output of basic sciences and clinical research and interdisciplinary patient care. PMID:19288314

  20. Ecologically based targets for bioavailable (reactive) nitrogen discharge from the drainage basins of the Wet Tropics region, Great Barrier Reef.

    PubMed

    Wooldridge, Scott A; Brodie, Jon E; Kroon, Frederieke J; Turner, Ryan D R

    2015-08-15

    A modelling framework is developed for the Wet Tropics region of the Great Barrier Reef that links a quantitative river discharge parameter (viz. dissolved inorganic nitrogen concentration, DIN) with an eutrophication indicator within the marine environment (viz. chlorophyll-a concentration, chl-a). The model predicts catchment-specific levels of reduction (%) in end-of-river DIN concentrations (as a proxy for total potentially reactive nitrogen, PRN) needed to ensure compliance with chl-a 'trigger' guidelines for the ecologically distinct, but PRN-related issues of crown-of-thorns starfish (COTS) outbreaks, reef biodiversity loss, and thermal bleaching sensitivity. The results indicate that even for river basins dominated by agricultural land uses, quite modest reductions in end-of-river PRN concentrations (∼20-40%) may assist in mitigating the risk of primary COTS outbreaks from the mid-shelf reefs of the Wet Tropics. However, more significant reductions (∼60-80%) are required to halt and reverse declines in reef biodiversity, and loss of thermal bleaching resistance. PMID:26072049

  1. Block-copolymer vesicles as nanoreactors for enzymatic reactions.

    PubMed

    Chen, Qi; Schönherr, Holger; Vancso, G Julius

    2009-06-01

    The impact of the spatial confinement of polystyrene-block-poly(acrylic acid) (PS-b-PAA) block copolymer (BCP) vesicles on the reactivity of encapsulated bovine pancreas trypsin is studied. Enzymes, as well as small molecules, are encapsulated with loading efficiencies up to 30% in BCP vesicles with variable internal volumes between 0.014 aL (internal vesicle diameter, d(in) = 30 nm) and 8 aL (d(in) = 250 nm), obtained by manipulating the vesicle preparation conditions. The kinetics of the trypsin-catalyzed reaction of a fluorogenic substrate inside and outside the vesicles is quantitatively estimated using fluorescence spectroscopic analyses in conjunction with the use of NaNO(2) as selective quencher for non-encapsulated fluorophores. The values of the catalytic turnover number obtained for reactions in differently sized nanoscale reactors show a significant increase (up to approximately 5x) with decreasing BCP vesicle volume, while the values of the Michaelis-Menten constant decrease. The observed increase in enzyme efficiency by two orders of magnitude compared to bulk solution is attributed to an enhanced rate of enzyme-substrate and molecule-wall collisions inside the nanosized reactors, as predicted in the literature on the basis of Monte Carlo simulations. PMID:19283796

  2. Enantiomers of myo-inositol-1,3,4-trisphosphate and myo-inositol-1,4,6 -trisphosphate: stereospecific recognition by cerebellar and platelet myo-inositol-1,4,5-trisphosphate receptors.

    PubMed

    Murphy, C T; Bullock, A J; Lindley, C J; Mills, S J; Riley, A M; Potter, B V; Westwick, J

    1996-11-01

    The naturally occurring tetrakisphosphate myo-inositol-1,3,4, 6-tetrakisphosphate [Ins(1,3,4,6)P4] was able to release Ca2+ from the intracellular stores of permeabilized rabbit platelets but was 40-fold less potent than D-myo-inositol-1,4,5-trisphosphate [Ins(1,4,5)P3]. The Ca2+ releasing activity of Ins(1,3,4,6)P4 was rationalized by envisaging two alternative receptor binding orientations in which the vicinal D-1,6-bisphosphate of Ins(1,3,4,6)P4 mimics the D-4,5-bisphosphate in the Ins(1,4,5)P3 binding conformation. This rationalization predicted that Ins(1,4,5)P3 regioisomers [i.e, D-myo-inositol -1,4,6-trisphosphate [D-Ins(1,4,6)P3] and D-myo-inositol-1,3,6 -trisphosphate [D-Ins(1,3,6)P3

  3. [The suitability of commercial bioindicators with spores of B. stearothermophilus for the testing of formaldehyde gas sterilizers].

    PubMed

    Mecke, P; Christiansen, B; Pirk, A

    1991-09-01

    Commercially available biological indicators with spores of B. stearothermophilus were investigated by the Hygiene-Institutes of Kiel and Lubeck. The objective was to find out if those indicators to which sheep blood was added subsequently correspond to the formaldehyde resistance required by. DIN 58948, part 14 (DIN 58948, part 13). Both working groups determined unanimously that the indicators of one producer showed a resistance too low compared to the remaining biological indicators showing a much higher resistance than required. Even biological indicators manufactured strictly in accordance to the testing standard were more resistant than demanded. This also corresponded to the commercially available untreated spores. On the other hand, practice showed that the biological indicators investigated within this study can be easily killed by formaldehyde sterilizers if they respond to the technical standard. In order to realize the testing of these sterilizers with indicators of a generally accepted resistance we propose either to demand for an equivalently higher formaldehyde resistance or to set up a killing period for the spore resistance from 150 to 240 min until experimentally important data are available. Concerning the blood containing indicators the results of both working groups differed considerably within the limits of formaldehyde efficiency whereas this was not the case with untested commercially available spores. As the addition of thinned blood did not cause an increase in resistance we recommend, in the interest of standardized investigative conditions, not to use it. PMID:1953931

  4. [Possible Instrument Contamination in the Operating Room During Implantation of Knee and Hip Arthroplasty].

    PubMed

    Quint, U; Benen, T

    2016-04-01

    Integrated ventilation systems with low turbulence displacement flow (TAV) are generally legally required in the architectural structure of operating theatres. However, it seems that the instruments laid out on sterile covered tables do not have the best possible protection from bacteria. Within an operating theatre, different bacteria counts are possible on the instruments. This prospective controlled study was conducted to demonstrate the influence of instrument tables with integrated horizontal flow on contamination with pathogens in comparison with conventional tables. In an operating theatre (OT) with a ceiling legally appropriate for TAV (2.40 m × 1.20 m), microbiological samples were placed on a table with integrated TAV flow (n = 100) and on a conventional instrument table (n = 100). The routine qualification of the OT was on an ongoing basis and was in accordance with DIN 1946-4: 1999 standards (in accordance with DIN measurement of recovery time 1946-4: 12-2008). This corresponds to the OT of the room class Ib. The results show significant differences between the two tables. The bacteria count and the percentage of contamination were many times higher on the conventional table. It is important to understand that the instruments are not completely protected against contamination after opening the pack and during the operation. Remedial measures are possible to optimise the sterility the instrument table. PMID:26844853

  5. Denitrification, leaching, and river nitrogen export in the Community Earth System Model

    NASA Astrophysics Data System (ADS)

    Nevison, Cynthia; Hess, Peter; Riddick, Stuart; Ward, Dan

    2016-03-01

    River nitrogen export is simulated within the Community Earth System Model (CESM) by coupling nitrogen leaching and runoff fluxes from the Community Land Model (CLM) to the River Transport Model (RTM). The coupled CLM-RTM prognostically simulates the downstream impact of human N cycle perturbation on coastal areas. It also provides a framework for estimating denitrification fluxes of N2 and associated trace gases like N2O in soils and river sediments. An important limitation of the current model is that it only simulates dissolved inorganic nitrogen (DIN) river export, due to the lack of dissolved organic nitrogen (DON) and particulate nitrogen (PN) leaching fluxes in CLM. In addition, the partitioning of soil N loss in CLM between the primary loss pathways of denitrification and N leaching/runoff appears heavily skewed toward denitrification compared to other literature estimates, especially in nonagricultural regions, and also varies considerably among the four model configurations presented here. River N export is generally well predicted in the model configurations that include midlatitude crops, but tends to be underpredicted in rivers that are less perturbed by human agriculture. This is especially true in the tropics, where CLM likely underestimates leaching and runoff of all forms of nitrogen. River export of DIN is overpredicted in some relatively unperturbed Arctic rivers, which may result from excessive N inputs to those regions in CLM. Better representation of N loss in CLM can improve confidence in model results with respect to the core model objective of simulating nitrogen limitation of the carbon cycle.

  6. Telomere-associated proteins add deoxynucleotides to terminal proteins during replication of the telomeres of linear chromosomes and plasmids in Streptomyces

    PubMed Central

    Yang, Chien-Chin; Tseng, Shu-Min; Chen, Carton W.

    2015-01-01

    Typical telomeres of linear chromosomes and plasmids of soil bacteria Streptomyces consist of tightly packed palindromic sequences with a terminal protein (‘TP’) covalently attached to the 5′ end of the DNA. Replication of these linear replicons is initiated internally and proceeds bidirectionally toward the telomeres, which leaves single-strand overhangs at the 3′ ends. These overhangs are filled by DNA synthesis using the TPs as the primers (‘end patching’). The gene encoding for typical TP, tpg, forms an operon with tap, encoding an essential telomere-associated protein, which binds TP and the secondary structures formed by the 3′ overhangs. Previously one of the two translesion synthesis DNA polymerases, DinB1 or DinB2, was proposed to catalyze the protein-primed synthesis. However, using an in vitro end-patching system, we discovered that Tpg and Tap alone could carry out the protein-primed synthesis to a length of 13 nt. Similarly, an ‘atypical’ terminal protein, Tpc, and its cognate telomere-associated protein, Tac, of SCP1 plasmid, were sufficient to achieve protein-primed synthesis in the absence of additional polymerase. These results indicate that these two telomere-associated proteins possess polymerase activities alone or in complex with the cognate TPs. PMID:25883134

  7. Genetic analysis of Escherichia coli RadA: functional motifs and genetic interactions

    PubMed Central

    Cooper, Deani L; Boyle, Daniel C; Lovett, Susan T

    2015-01-01

    The RadA/Sms protein is a RecA-related protein found universally in eubacteria and plants, implicated in processing of recombination intermediates. Here we show that the putative Zn finger, Walker A motif, KNRXG motif and Lon protease homology domain of the Escherichia coli RadA protein are required for DNA damage survival. RadA is unlikely to possess protease activity as the putative active site serine is not required. Mutants in RadA have strong synergistic phenotypes with those in the branch migration protein RecG. Sensitivity of radA recG mutants to azidothymidine (AZT) can be rescued by blocking recombination with recA or recF mutations or by overexpression of RuvAB, suggesting that lethal recombination intermediates accumulate in the absence of RadA and RecG. Synthetic genetic interactions for survival to AZT or ciprofloxacin exposure were observed between RadA and known or putative helicases including DinG, Lhr, PriA, Rep, RuvAB, UvrD, YejH and YoaA. These represent the first affected phenotypes reported for Lhr, YejH and YoaA. The specificity of these effects sheds new light on the role of these proteins in DNA damage avoidance and repair and implicates a role in replication gap processing for DinG and YoaA and a role in double-strand break repair for YejH. PMID:25484163

  8. Effect of active screen plasma nitriding pretreatment on wear behavior of TiN coating deposited by PACVD technique

    NASA Astrophysics Data System (ADS)

    Raoufi, M.; Mirdamadi, Sh.; Mahboubi, F.; Ahangarani, Sh.; Mahdipoor, M. S.; Elmkhah, H.

    2012-08-01

    Titanium based alloys are used extensively for improving wear properties of different parts due to their high hardness contents. Titanium nitride (TiN) is among these coatings which can be deposited on surface using various techniques such as CVD, PVD and PACVD. Their weak interface with substrate is one major drawback which can increase the total wear in spite of favorite wear behavior of TiN. Disc shaped samples from AISI H13 (DIN 1.2344) steel were prepared in this study. Single TiN coating was deposited on some of them while others have experienced a TiN deposition by active screen plasma nitriding (ASPN). Hardness at the surface and depth of samples was measured through Vickers micro hardness test which revealed 1810 Hv hardness as the maximum values for a dual-layered ASPN-TiN. Pin-on-disc wear test was done in order to study the wear mechanism. In this regard, the wear behavior of samples was investigated against pins from 100Cr6 (Din 1.3505) bearing steel and tungsten carbide-cobalt (WC-Co) steel. It was evidenced that the dual-layer ASPN-TiN coating has shown the least weight loss with the best wearing behavior because of its high hardness values, stable interface and acceptable resistance against peeling during wearing period.

  9. Quantum tunneling and vibrational dynamics of ultra-confined water

    NASA Astrophysics Data System (ADS)

    Kolesnikov, Alexander I.; Anovitz, Lawrence M.; Ehlers, Georg; Mamontov, Eugene; Podlesnyak, Andrey; Prisk, Timothy R.; Seel, Andrew; Reiter, George F.

    2015-03-01

    Vibrational dynamics of ultra-confined water in single crystals beryl, the structure of which contains ~ 5 Å diameter channels along the c-axis was studied with inelastic (INS), quasi-elastic (QENS) and deep inelastic (DINS) neutron scattering. The results reveal significantly anisotropic dynamical behavior of confined water, and show that effective potential experienced by water perpendicular to the channels is significantly softer than along them. The observed 7 peaks in the INS spectra (at energies 0.25 to 15 meV), based on their temperature and momentum transfer dependences, are explained by transitions between the split ground states of water in beryl due to water quantum tunneling between the 6-fold equivalent positions across the channels. DINS study of beryl at T=4.3 K shows narrow, anisotropic water proton momentum distribution with corresponding kinetic energy, EK=95 meV, which is much less than was previously observed in bulk water (~150 meV). We believe that the exceptionally small EK in beryl is a result of water quantum tunneling ∖ delocalization in the nanometer size confinement and weak water-cage interaction. The neutron experiment at ORNL was sponsored by the Sci. User Facilities Div., BES, U.S. DOE. This research was sponsored by the Div. Chemical Sci, Geosciences, and Biosciences, BES, U.S. DOE. The STFC RAL is thanked for access to ISIS neutron facilities.

  10. A sustainability model based on cloud infrastructures for core and downstream Copernicus services

    NASA Astrophysics Data System (ADS)

    Manunta, Michele; Calò, Fabiana; De Luca, Claudio; Elefante, Stefano; Farres, Jordi; Guzzetti, Fausto; Imperatore, Pasquale; Lanari, Riccardo; Lengert, Wolfgang; Zinno, Ivana; Casu, Francesco

    2014-05-01

    The incoming Sentinel missions have been designed to be the first remote sensing satellite system devoted to operational services. In particular, the Synthetic Aperture Radar (SAR) Sentinel-1 sensor, dedicated to globally acquire over land in the interferometric mode, guarantees an unprecedented capability to investigate and monitor the Earth surface deformations related to natural and man-made hazards. Thanks to the global coverage strategy and 12-day revisit time, jointly with the free and open access data policy, such a system will allow an extensive application of Differential Interferometric SAR (DInSAR) techniques. In such a framework, European Commission has been funding several projects through the GMES and Copernicus programs, aimed at preparing the user community to the operational and extensive use of Sentinel-1 products for risk mitigation and management purposes. Among them, the FP7-DORIS, an advanced GMES downstream service coordinated by Italian National Council of Research (CNR), is based on the fully exploitation of advanced DInSAR products in landslides and subsidence contexts. In particular, the DORIS project (www.doris-project.eu) has developed innovative scientific techniques and methodologies to support Civil Protection Authorities (CPA) during the pre-event, event, and post-event phases of the risk management cycle. Nonetheless, the huge data stream expected from the Sentinel-1 satellite may jeopardize the effective use of such data in emergency response and security scenarios. This potential bottleneck can be properly overcome through the development of modern infrastructures, able to efficiently provide computing resources as well as advanced services for big data management, processing and dissemination. In this framework, CNR and ESA have tightened up a cooperation to foster the use of GRID and cloud computing platforms for remote sensing data processing, and to make available to a large audience advanced and innovative tools for DIn

  11. Macroalgae Mediation of Dissolved Organic Nitrogen Fluxes in a Temperate Coastal Lagoon

    NASA Astrophysics Data System (ADS)

    Tyler, A. C.; McGlathery, K. J.; Anderson, I. C.

    2001-08-01

    The activity of the benthos, including benthic plants, is important in driving the overall system dynamics in shallow lagoons, due to the high ratio of sediment surface area relative to water volume. In Hog Island Bay, benthic macroalgae appear to be a key regulator of DON dynamics, both while alive and following senescence. We investigated the role of macroalgae in mediating water column concentrations and sediment-water column fluxes of DON across a nutrient gradient in Hog Island Bay, a shallow macroalgal-dominated back-barrier lagoon located on the Virginia Coast. Sediment-water column exchanges of DON, urea and DIN were measured in sediment cores with and without macroalgae ( Ulva lactuca) at three subtidal sites from the mainland to the barrier islands in the fall of 1997 and the spring and summer of 1998. The summer sampling dates bracketed a large macroalgal bloom in the mid-lagoon. Dissolved organic nitrogen was an important component (52-98%) of the total dissolved nitrogen pool in Hog Island Bay waters and made up the majority of the sediment N flux to the water column. Macroalgae impacted benthic-pelagic coupling by preventing diffusion of DIN from the water column to the sediments and by intercepting urea fluxes from the sediment to the water column. Closest to the mainland and closest to the barrier islands, at sites with low macroalgal biomass, sediment-water column fluxes of DIN and urea-free DON were negligible or directed into the sediments. Fluxes of urea from the sediment to the water column were significant at both sites, and may play an important role in satisfying macroalgal N demand, especially at the low N island site. Overall, urea was 32% of the mean DON flux from the sediments to the water column. Fluxes of urea-free DON were highest in the mid-lagoon, where macroalgal biomass was highest. The highest overall flux rates of DON (>38 mmol m -2 d -1) and DIN (>33 mmol m -2 d -1) were measured following an isolated crash of a large

  12. Dynamic seasonal nitrogen cycling in response to anthropogenic N loading in a tropical catchment, Athi-Galana-Sabaki River, Kenya

    NASA Astrophysics Data System (ADS)

    Marwick, T. R.; Tamooh, F.; Ogwoka, B.; Teodoru, C.; Borges, A. V.; Darchambeau, F.; Bouillon, S.

    2014-01-01

    As part of a broader study on the riverine biogeochemistry in the Athi-Galana-Sabaki (A-G-S) River catchment (Kenya), we present data constraining the sources, transit and transformation of multiple nitrogen (N) species as they flow through the A-G-S catchment (~47 000 km2). The data set was obtained in August-September 2011, November 2011, and April-May 2012, covering the dry season, short rain season and long rain season respectively. Release of (largely untreated) wastewater from the city of Nairobi had a profound impact on the biogeochemistry of the upper Athi River, leading to low dissolved oxygen (DO) saturation levels (36-67%), high ammonium (NH4+) concentrations (123-1193 μmol L-1), and high dissolved methane (CH4) concentrations (3765-6729 nmol L-1). Riverine dissolved inorganic nitrogen (DIN; sum of NH4+ and nitrate (NO3-); nitrite was not measured) concentration at the most upstream site on the Athi River was highest during the dry season (1195 μmol L-1), while DIN concentration was an order of magnitude lower during the short and long rain seasons (212 and 193 μmol L-1, respectively). During the rain seasons, low water residence time led to relatively minimal in-stream N cycling prior to discharge to the ocean, whereas during the dry season we speculate that prolonged residence time creates two differences comparative to wet season, where (1) intense N cycling and removal of DIN is possible in the upper to mid-catchment and leads to significantly lower concentrations at the outlet during the dry season, and (2) as a result this leads to the progressive enrichment of 15N in the particulate N (PN) pool, highlighting the dominance of untreated wastewater as the prevailing source of riverine DIN. The rapid removal of NH4+ in the upper reaches during the dry season was accompanied by a quantitatively similar production of NO3- and nitrous oxide (N2O) downstream, pointing towards strong nitrification over this reach during the dry season. Nitrous oxide

  13. Modelling the relative impact of rivers (Scheldt/Rhine/Seine) and Western Channel waters on the nutrient and diatoms/ Phaeocystis distributions in Belgian waters (Southern North Sea)

    NASA Astrophysics Data System (ADS)

    Lacroix, Geneviève; Ruddick, Kevin; Gypens, Nathalie; Lancelot, Christiane

    2007-06-01

    The coastal areas of the Southern North Sea (SNS) experience eutrophication problems resulting from freshwater nitrogen (N) and phosphorus (P) inputs from rivers. In particular, massive blooms of Phaeocystis colonies occur in Belgian waters. In this region, water masses result from the mixing of Western Channel (WCH) waters transported through the Straits of Dover with nutrient-rich freshwater from the Scheldt, the Rhine and Meuse, the Seine, the Thames and other smaller rivers. However, the relative contribution of the WCH and each river to the inorganic nutrient pool and the impact on the phytoplankton community structure (diatoms and Phaeocystis) are not known. In order to effectively manage the eutrophication problems, it is necessary to know: (i) the relative contribution of the WCH and of each river impacting the region and (ii) the relative effect of a N and/or P nutrient reduction on the Phaeocystis blooms. To answer these questions, sensitivity tests (1% nutrient reduction) and nutrient reduction scenarios (50% nutrient reduction) have been performed with a three-dimensional (3D) coupled physical-biogeochemical model (MIRO&CO-3D). MIRO&CO-3D results from the coupling of the COHERENS 3D hydrodynamic model with the ecological model MIRO. The model has been set up for the region between 48.5°N, 4°W and 52.5°N, 4.5°E and run to simulate the annual cycle of carbon, inorganic and organic nutrients, phytoplankton (diatoms and Phaeocystis), bacteria and zooplankton (microzooplankton and copepods) in the SNS under realistic forcing (meteorology and river inputs) for the period 1991-2003. The relative contribution of the WCH waters and of the different rivers on the inorganic nutrient pool available for phytoplankton (diatoms and Phaeocystis) growth is assessed by decreasing by 1% the nutrient (dissolved inorganic nitrogen, DIN and inorganic phosphate, PO 4) inputs from the WCH and from, respectively, the Scheldt (and smaller Belgian rivers), the Rhine/Meuse and

  14. Net subterranean estuarine export fluxes of dissolved inorganic C, N, P, Si, and total alkalinity into the Jiulong River estuary, China

    NASA Astrophysics Data System (ADS)

    Wang, Guizhi; Wang, Zhangyong; Zhai, Weidong; Moore, Willard S.; Li, Qing; Yan, Xiuli; Qi, Di; Jiang, Yuwu

    2015-01-01

    To evaluate geochemical impacts of the subterranean estuary (STE) on the Jiulong River estuary, China, we estimated seasonal fluxes of subterranean water discharge into the estuary based on the mass balance of radium isotopes and net subterranean export fluxes of dissolved inorganic C (DIC), N (DIN), Si (DSi), soluble reactive phosphorus (SRP), and total alkalinity (TA). Based on 226Ra data, the subterranean discharge (in 107 m3 d-1) was estimated to be 0.29-0.60 in the spring, 0.69-1.44 in the summer, 0.45-0.93 in the fall, and 0.26-0.54 in the winter. This was equivalent to 8-19% of the concomitant river discharge. The net spatially integrated material fluxes from the STE into the estuary were equivalent up to 45-110% of the concomitant riverine fluxes for DIC and TA, around 14-32% for DSi and 7-19% for DIN, and negligible for SRP. Paradoxically, the mixing lines along the salinity gradient revealed no apparent additions of these species. These additions are not revealed because the STE is a relatively small spatially-averaged source (at most 11% of the total input at steady state) that spreads throughout the estuary as a non-point source in contrast to the major point sources of the river and the ocean for the estuary and a true open ocean endmember is likely lacking. Greater water flushing in the summer might dilute the STE effect on the mixing lines even more. The great spatial variation in salinity in the estuary introduced the major uncertainty in our estimates of the flushing time, which further affected the estimate of the subterranean discharge and associated material fluxes. Additionally, the great spatial variation in the STE endmember caused the relatively large ranges in these flux estimates. Despite apparent conservative mixing of DIC, DIN, and DSi in estuaries, net subterranean exports must be taken into account in evaluating geochemical impacts of estuarine exports on shelf waters.

  15. Primary, new and export production in the NW Pacific subarctic gyre during the vertigo K2 experiments

    NASA Astrophysics Data System (ADS)

    Elskens, M.; Brion, N.; Buesseler, K.; Van Mooy, B. A. S.; Boyd, P.; Dehairs, F.; Savoye, N.; Baeyens, W.

    2008-07-01

    This paper presents results on tracer experiments using 13C and 15N to estimate uptake rates of dissolved inorganic carbon (DIC) and nitrogen (DIN). Experiments were carried out at station K2 (47°N, 161°E) in the NW Pacific subarctic gyre during July-August 2005. Our goal was to investigate relationships between new and export production. New production was inferred from the tracer experiments using the f ratio concept (0-50 m); while export production was assessed with neutrally buoyant sediment traps (NBSTs) and the e ratio concept (at 150 m). During trap deployments, K2 was characterized both by changes in primary production (523-404 mg C m -2 d -1), new production (119-67 mg C m -2 d -1), export production (68-24 mg C m -2 d -1) and phytoplankton composition (high to low proportion of diatoms). The data indicate that 17-23% of primary production is exportable to deeper layers ( f ratio) but only 6-13% collected as a sinking particle flux at 150 m ( e ratio). Accordingly, >80% of the carbon fixed by phytoplankton would be mineralized in the upper 50 m (1- f), while <11% would be within 50-150 m ( f- e). DIN uptake flux amounted to 0.5 mM m -2 h -1, which was equivalent to about 95% particulate nitrogen (PN) remineralized and/or grazed within the upper 150 m. Most of the shallow PN remineralization occurred just above the depth of the deep chlorophyll maximum (DCM), where a net ammonium production was measured. Below the DCM, while nitrate uptake rates became negligible because of light limitation, ammonium uptake did continue to be significant. The uptake of ammonium by heterotrophic bacteria was estimated to be 14-17% of the DIN assimilation. Less clear are the consequences of this uptake on the phytoplankton community and biogeochemical processes, e.g. new production. It was suggested that competition for ammonium could select for small cells and may force large diatoms to use nitrate. This implies that under Fe stress as observed here, ammonium uptake is

  16. Setting background nutrient levels for coastal waters with oceanic influences

    NASA Astrophysics Data System (ADS)

    Smith, Alastair F.; Fryer, Rob J.; Webster, Lynda; Berx, Bee; Taylor, Alison; Walsham, Pamela; Turrell, William R.

    2014-05-01

    Nutrient enrichment of coastal water bodies as a result of human activities can lead to ecological changes. As part of a strategy to monitor such changes and detect potential eutrophication, samples were collected during research cruises conducted around the Scottish coast each January over the period 2007-2013. Data were obtained for total oxidised nitrogen (TOxN; nitrite and nitrate), phosphate and silicate, and incorporated into data-driven spatial models. Spatial averages in defined sea areas were calculated for each year in order to study inter-annual variability and systematic trends over time. Variation between some years was found to be significant (p < 0.05) but no evidence was found for any trends over the time period studied. This may have been due to the relatively short time series considered here. Modelled distributions were developed using data from groups of years (2007-2009, 2010-2011 and 2012-2013) and compared to the OSPAR Ecological Quality Objectives (EcoQOs) for dissolved inorganic nitrogen (DIN; the concentration of TOxN and ammonia), the ratio of DIN to dissolved inorganic phosphorous (N/P) and the ratio of DIN to dissolved silicate (N/S). In these three models, TOxN was below the offshore background concentration of 10 μM (12 μM at coastal locations) over more than 50% of the modelled area while N/S exceeded the upper assessment criterion of 2 over more than 50% of the modelled area. In the 2007-2009 model, N/P was below the background ratio (16) over the entire modelled area. In the 2010-2011 model the N/P ratio exceeded the background in 91% of the modelled area but remained below the upper assessment criterion (24). Scottish shelf sea waters were found to be depleted in TOxN relative to oceanic waters. This was not accounted for in the development of background values for the OSPAR EcoQOs so new estimates of these background values were derived. The implications of these results for setting reasonable background nutrient levels when

  17. Multi-species measurements of nitrogen isotopic composition reveal the spatial constraints and biological drivers of ammonium attenuation across a highly contaminated groundwater system.

    PubMed

    Wells, Naomi S; Hakoun, Vivien; Brouyère, Serge; Knöller, Kay

    2016-07-01

    Groundwater under industrial sites is characterised by heterogeneous chemical mixtures, making it difficult to assess the fate and transport of individual contaminants. Quantifying the in-situ biological removal (attenuation) of nitrogen (N) is particularly difficult due to its reactivity and ubiquity. Here a multi-isotope approach is developed to distinguish N sources and sinks within groundwater affected by complex industrial pollution. Samples were collected from 70 wells across the two aquifers underlying a historic industrial area in Belgium. Below the industrial site the groundwater contained up to 1000 mg N l(-1) ammonium (NH4(+)) and 300 mg N l(-1) nitrate (NO3(-)), while downgradient concentrations decreased to ∼1 mg l(-1) DIN ([DIN] = [NH4(+)N] + [NO3(-)N] + [NO2(-)N]). Mean δ(15)N-DIN increased from ∼2‰ to +20‰ over this flow path, broadly confirming that biological N attenuation drove the measured concentration decrease. Multi-variate analysis of water chemistry identified two distinct NH4(+) sources (δ(15)NNH4(+) from -14‰ and +5‰) within the contaminated zone of both aquifers. Nitrate dual isotopes co-varied (δ(15)N: -3‰ - +60‰; δ(18)O: 0‰ - +50‰) within the range expected for coupled nitrification and denitrification of the identified sources. The fact that δ(15)NNO2(-) values were 50‰-20‰ less than δ(15)NNH4(+) values in the majority of wells confirmed that nitrification controlled N turnover across the site. However, the fact that δ(15)NNO2(-) was greater than δ(15)NNH4(+) in wells with the highest [NH4(+)] shows that an autotrophic NO2(-) reduction pathway (anaerobic NH4(+) oxidation or nitrifier-denitrification) drove N attenuation closest to the contaminant plume. This direct empirical evidence that both autotrophic and heterotrophic biogeochemical processes drive N attenuation in contaminated aquifers demonstrates the power of multiple N isotopes to untangle N cycling in highly complex

  18. D0 Silicon Upgrade: CTF Front End Crate J2/J3 BAckplane Specification

    SciTech Connect

    Baert, Mark; /Fermilab

    1995-07-03

    GENERAL PHYSICAL LAYOUT: (1) The backplane is comprised of 2 identical sections, the upper half designated J1, and the lower half designated j3; (2) For each section, there are a total of 16 slots, designated 1 through 16, from left to right; (3) Odd numbered slots are left handed (Connectors to the left of the card), these slots utilize inverse DIN connectors; (4) Even numbered slots are right handed (Connectors to the right of the card), these slots utilize standard DIN connectors; (5) There are 2 60 pin daisy-chain headers associated with each section, 3M part number 3597-6003; (6) The headers associated with the J2 (upper) section are designated J2CHAINR and J2CHAINL; (7) The headers associated with the J3 (lower) section are designated J3CHAINL and J3CHAINR; and (8) Mechanical layout presented in drawing number 3823.113-MD-330045. TRIGGER SIGNALS: (1) There are 21 trigger signal which originate from a given slot and are routed to the slot immediately to the left and 21 trigger signals which originate from a given slot and are routed to the slot immediately to the right; (2) There are 21 trigger signals which originate from the slot immediately to the left of a given slot and 21 trigger signals which originate from the slot immediately to the right a given slot; (3) The signal are designated TRIGjk-n, when j is the source slot, k is the destination slot, and n is the signal number; (4) These signals shall be implemented as 100 ohm microstip above ground plane; and (5) No terminations. DC POWER: (1) Each slot is supplied with DC power on the connector pins designated as VCC and GND; (2) VCC and GND originates at rear of the backplane via press-in power terminals, their locations are shown in drawing number 3823.113-MD-330045; (3) Power shall be bypassed at 2 locations with a 10uF tantalum capacitor in parallel with a .01uF 100V ceramic capacitor; and (4) The total ampacity supplied by each DIN connector is 3.6 amps.

  19. Carbon and Nitrogen cycling in a permafrost soil profile

    NASA Astrophysics Data System (ADS)

    Salmon, V. G.; Schaedel, C.; Mack, M. C.; Schuur, E.

    2015-12-01

    In high latitude ecosystems, active layer soils thaw during the growing season and are situated on top of perennially frozen soils (permafrost). Permafrost affected soil profiles currently store a globally important pool of carbon (1330-1580 PgC) due to cold temperatures constraining the decomposition of soil organic matter. With global warming, however, seasonal thaw is expected to increase in speed and extend to deeper portions of the soil profile. As permafrost soils become part of the active layer, carbon (C) and nitrogen (N) previously stored in soil organic matter will be released via decomposition. In this experiment, the dynamic relationship between N mineralization, C mineralization, and C quality was investigated in moist acidic tundra soils. Soils from the active layer surface down through the permafrost (80cm) were incubated aerobically at 15°C for 225 days. Carbon dioxide fluxes were fit with a two pool exponential decay model so that the size and turnover of both the quickly decomposing C pool (Cfast) and the slowly decomposing C pool (Cslow) could be assessed. Soil extractions with 2M KCl were performed at six time points throughout the incubation so that dissolve inorganic N (DIN) and dissolved organic C (DOC) could be measured. DIN was readily extractable from deep permafrost soils throughout the incubation (0.05 mgN/g dry soil) but in active layer soils DIN was only produced after Cfast had been depleted. In contrast, active layer soils had high levels of DOC (0.65 mgC/g dry soil) throughout the incubation but in permafrost soils, DOC became depleted as Cfast reduced in size. The strong contrasts between the C and N cycling in active layer soils versus permafrost soils suggest that the deeper thaw will dramatically increase N availability in these soil profiles. Plants and soil microbes in the tundra are currently N limited so our findings imply that deepening thaw will 1) provide N necessary for increased plant growth and 2) stimulate losses of

  20. Summer nutrient dynamics and biological carbon uptake rate in the Changjiang River plume inferred using a three end-member mixing model

    NASA Astrophysics Data System (ADS)

    Wang, Kui; Chen, Jianfang; Jin, Haiyan; Li, Hongliang; Gao, Shengquan; Xu, Jie; Lu, Yong; Huang, Daji; Hao, Qiang; Weng, Huanxin

    2014-12-01

    A three end-member (Changjiang River Plume, CRP; Outer-shelf Surface Water, OSW; and Outer-shelf Deep Water, ODW) mixing model based on quasi-conservative temperature and salinity was adopted to identify the relative contribution of different water masses to nutrient inventory and to estimate biological nutrient uptake in the plume-impacted area by considering the difference between the model-predicted and ambient concentration of nutrients up to a depth of PZD10 (photic zone depth to 10% of surface photosynthetically active radiance (PAR)). The end-member composite suggested that the mixing of CRP and OSW was the main process regulating nutrient dynamics and phytoplankton growth, while the correlation of ODW with ΔP indicated that the outcropped upwelling water contributed to the replenishment of P leading to Chl a accumulation to some extent. The ratio ΔDIN:ΔP:ΔSi:excess O2 in the euphotic zone where excess O2>10 μmol L-1 was estimated to be 28±9:1:33±13:145±71. A simple box model was used to evaluate biological carbon uptake rate in the euphotic zone based on nutrient deviation, Redfield ratio (6.6 C:1 DIN), and residence time of nutrients, assuming that the Changjiang River was the unique source of nutrients in the quasi-static box. The biological carbon uptake rates derived from the DIN, P and Si deviation were 465, 344, and 626 mg C m-2 d-1, respectively; these values were comparable to the POC flux (486±275 mg C m-2 d-1) derived from sediment trap. This finding suggested that the Changjiang River Plume was responsible for phytoplankton growth and subsequent high POC flux out of the euphotic zone. Furthermore, the community respiration rate was estimated to be 634 mg C m-2 d-1 based on the integrated 14C-based gross primary production of 1260 mg C m-2 d-1 and the net community production of 626 mg C m-2 d-1 in the euphotic zone of the region.

  1. Land use change and nitrogen enrichment of a Rocky Mountain watershed.

    PubMed

    Kaushal, Sujay S; Lewis, William M; McCutchan, James H

    2006-02-01

    Headwater ecosystems may have a limited threshold for retaining and removing nutrients delivered by certain types of land use. Nitrogen enrichment was studied in a Rocky Mountain watershed undergoing rapid expansion of population and residential development. Study sites were located along a 30-km transect from the headwaters of the Blue River to Lake Dillon, a major source of drinking water for Denver, Colorado. Ground water in residential areas with septic systems showed high concentrations of nitrate-N (4.96 +/- 1.22 mg/L, mean +/- SE), and approximately 40% of wells contained nitrate with delta15N values in the range of wastewater. Concentrations of dissolved inorganic nitrogen (DIN) in tributaries with residential development peaked during spring snowmelt as concentrations of DIN declined to below detection limits in undeveloped tributaries. Annual export of dissolved organic nitrogen (DON) was considerably lower in residential streams, suggesting a change in forms of N with development. The seasonal delta15N of algae in residential streams was intermediate between baseline values from undeveloped streams and stream algae grown on wastewater. Between 19% and 23% of the annual N export from developed tributaries was derived from septic systems, as estimated from the delta15N of algae. This range was similar to the amount of N export above background determined independently from mass-balance estimates. From a watershed perspective, total loading of N to the Blue River catchment from septic and municipal wastewater (2 kg x ha(-1) x yr(-1)) is currently less than the amount from background atmospheric sources (3 kg x ha(-1) x yr(-1)). Nonetheless, nitrate-N concentrations exceeded limits for safe drinking water in some groundwater wells (10 mg/L), residential streams showed elevated seasonal patterns of nitrate-N concentration and ratios of DIN to total dissolved phosphorus, and seasonal minimum concentrations of nitrate-N in Lake Dillon have increased

  2. Importance of within-lake processes in affecting the dynamics of dissolved organic carbon and dissolved organic and inorganic nitrogen in an Adirondack forested lake/watershed

    NASA Astrophysics Data System (ADS)

    Kang, P.-G.; Mitchell, M. J.; McHale, P. J.; Driscoll, C. T.; McHale, M. R.; Inamdar, S.; Park, J.-H.

    2015-10-01

    Lakes nested in forested watersheds play important roles in mediating the concentrations and fluxes of dissolved organic matter. We compared long-term patterns of concentrations and fluxes of dissolved organic carbon (DOC), dissolved organic nitrogen (DON), and dissolved inorganic nitrogen (DIN) in the Arbutus Lake Watershed to evaluate how a lake nested in a forested watershed affects the dynamics of DOC and DON in the Adirondack Mountains of New York State, USA. We observed no significant long-term changes of concentrations and fluxes of DOC and DON in the Lake outlet since 1983 and 1994, respectively. However, the temporal patterns of DOC and DON concentrations in the Lake inlet showed significant seasonality such as increases during the vegetation-growing season along with notable decreases in the dormant season. A comparison of mass-balances between inlet and outlet for the period from 2000 to 2009 suggested that the Lake was a sink of DOC (mean of influx minus outflux: +1140 mol C ha-1 yr-1). In contrast, the difference of discharge-weighted DON concentrations (mean of inlet minus outlet: -1.0 μmol N L-1) between inlet and outlet was much smaller than the discharge-weighted DOC concentrations (average of inlet minus outlet: +87 μmol C L-1). DON fluxes showed considerable variation among years (mean of influx minus outflux: +8 mol N ha-1 yr-1; range of differences: -15 to 27 mol N ha-1 yr-1). DON exhibited low % retention ((influx - outflux) / influx) (mean: 6.9 %, range: -34.8 to +31.2) compared to DOC (mean: 30.1 %, range: +9.2 to +44.1). The resultant increase of DON within the lake was closely linked with a net decrease of DIN through monthly Pearson correlation analysis, suggesting the importance of biotic factors in mediating a lake DON dynamics. Our results show different relative retentions of DOC compared with DON, along with a larger retention of DIN than DON, suggesting that DOC and DON might display substantially different biogeochemical

  3. Hydrological and Biogeochemical Controls on Seasonal and Spatial Differences in Food Webs in the Everglades

    NASA Astrophysics Data System (ADS)

    Kendall, C.; Wankel, S. D.; Bemis, B. E.; Rawlik, P. S.; Krabbenhoft, D. P.; Lange, T.

    2002-05-01

    Stable isotopes can be used to determine the relative trophic positions of biota within a food web, and to improve our understanding of the biomagnification of contaminants. Plants at the base of the food web uptake dissolved organic carbon (DIC) and nitrogen (DIN) for growth, and their tissue reflects the isotopic composition of these sources. Animals then mirror the isotopic composition of the primary producers, as modified by consumer-diet fractionations at successive trophic steps. During 1995-99, we collected algae, macrophyte, invertebrate, and fish samples from 15 USGS sites in the Everglades and analyzed them for d13C and d15N with the goal of characterizing seasonal and spatial differences in food web relations. Carbon isotopes effectively distinguish between two main types of food webs: ones where algae is the dominant base of the food web, which are characteristic of relatively pristine marsh sites with long hydroperiods, and ones where macrophyte debris appears to be a significant source of nutrients, which are apparently characteristic of shorter hydroperiod sites, and nutrient-impacted marshes and canals. There usually is an inverse relation between d13C and d15N of organisms over time, especially in more pristine environments, reflecting seasonal changes in the d13C of DIC and the d15N of DIN. The d13C and d15N of algae also show strong positive correlations with seasonal changes in water levels. This variability is substantially damped up the food chain, probably because of the longer integration times of animals vs. plants. We speculate that these seasonal shifts in water level result in changes in biogeochemical reactions and nutrient levels, with corresponding variations in the d15N and d13C of biota. For example, small changes in water level may change the balance of photosynthesis, bacterial respiration, and atmospheric exchange reactions that control the d13C of DIC. Such changes will probably also affect the d15N of dissolved inorganic N (DIN

  4. Reduction of Airborne Bacterial Burden in the OR by Installation of Unidirectional Displacement Airflow (UDF) Systems

    PubMed Central

    Fischer, Sebastian; Thieves, Martin; Hirsch, Tobias; Fischer, Klaus-Dieter; Hubert, Helmine; Bepler, Steffen; Seipp, Hans-Martin

    2015-01-01

    Background Intraoperative bacterial contamination is a major risk factor for postoperative wound infections. This study investigated the influence of type of ventilation system on intraoperative airborne bacterial burden before and after installation of unidirectional displacement air flow systems. Material/Methods We microbiologically monitored 1286 surgeries performed by a single surgical team that moved from operating rooms (ORs) equipped with turbulent mixing ventilation (TMV, according to standard DIN-1946-4 [1999], ORs 1, 2, and 3) to ORs with unidirectional displacement airflow (UDF, according to standard DIN-1946-4, annex D [2008], ORs 7 and 8). The airborne bacteria were collected intraoperatively with sedimentation plates. After incubation for 48 h, we analyzed the average number of bacteria per h, peak values, and correlation to surgery duration. In addition, we compared the last 138 surgeries in ORs 1–3 with the first 138 surgeries in ORs 7 and 8. Results Intraoperative airborne bacterial burden was 5.4 CFU/h, 5.5 CFU/h, and 6.1 CFU/h in ORs 1, 2, and 3, respectively. Peak values of burden were 10.7 CFU/h, 11.1 CFU/h, and 11.0 CFU/h in ORs 1, 2, and 3, respectively). With the UDF system, the intraoperative airborne bacterial burden was reduced to 0.21 CFU/h (OR 7) and 0.35 CFU/h (OR 8) on average (p<0.01). Accordingly, peak values decreased to 0.9 CFU/h and 1.0 CFU/h in ORs 7 and 8, respectively (p<0.01). Airborne bacterial burden increased linearly with surgery duration in ORs 1–3, but the UDF system in ORs 7 and 8 kept bacterial levels constantly low (<3 CFU/h). A comparison of the last 138 surgeries before with the first 138 surgeries after changing ORs revealed a 94% reduction in average airborne bacterial burden (5 CFU/h vs. 0.29 CFU/h, p<0.01). Conclusions The unidirectional displacement airflow, which fulfills the requirements of standard DIN-1946-4 annex D of 2008, is an effective ventilation system that reduces airborne bacterial burden

  5. Runoff nutrient and suspended sediment fluxes, cycling, and management in southern Kaneohe Bay, Hawaii

    NASA Astrophysics Data System (ADS)

    Ringuet, S.; Young, C. W.; Hoover, D. J.; de Carlo, E. H.; MacKenzie, F. T.

    2003-12-01

    Urban runoff and its impact on water quality in Hawaii, especially after heavy rainfall, is highly dynamic. In the past, water quality was determined through "grab samples" that were merely snapshots in time of an ever-changing environment. In contrast, continuous measurements of water quality can capture data that reflect the effects of significant storm runoff events unobtainable using even frequent manual sampling. Continuous multiparameter monitoring facilitates investigation of the both the magnitude and persistence of impacts of storm runoff on coastal waters, which can eventually be related to the health of coral reef ecosystems. Taking advantage of recent technological developments in oceanographic instrumentation, our study assembled an instrument package dubbed Coral Reef Instrumented Monitoring Platform (CRIMP). CRIMP was designed to include probes that measure physical and biological parameters (temperature, salinity, pH, dissolved oxygen, turbidity, and chlorophyll-a), nutrient analyzers (nitrate and phosphate), and a particle analyzer based on laser in-situ scattering and transmissometry. Various components of the CRIMP were previously used in conjunction with grab samples with the objective of elucidating the water quality of southern Kaneohe Bay and its relationship to physical, biological, and chemical processes operating in the bay, and to coral reef ecosystems. All instruments are now being combined on the CRIMP, and will allow us to study in near real time changes in fluvial inputs to the bay during storm runoff conditions and their impact on bay water quality and the coral reef ecosystem. In this presentation we discuss effects of freshwater delivery on adjacent coastal waters during high rainfall episodes (May 2002 and Feb 2003) that result in large runoff events and increased nutrient loading to coastal waters. Dissolved inorganic nitrogen to phosphorus ratios (DIN:DIP) in the Bay normally range from 2 to 4, suggesting a nitrogen

  6. Composition and fluxes of submarine groundwater along the Caribbean coast of the Yucatan Peninsula

    NASA Astrophysics Data System (ADS)

    Null, Kimberly A.; Knee, Karen L.; Crook, Elizabeth D.; de Sieyes, Nicholas R.; Rebolledo-Vieyra, Mario; Hernández-Terrones, Laura; Paytan, Adina

    2014-04-01

    Submarine groundwater discharge (SGD) to the coastal environment along the eastern Yucatan Peninsula, Quintana Roo, Mexico was investigated using a combination of tracer mass balances and analytical solutions. Two distinct submarine groundwater sources including water from the unconfined surficial aquifer discharging at the beach face and water from a deeper aquifer discharging nearshore through submarine springs (ojos) were identified. The groundwater of nearshore ojos was saline and significantly enriched in short-lived radium isotopes (223Ra, 224Ra) relative to the unconfined aquifer beach face groundwater. We estimated SGD from ojos using 223Ra and used a salinity mass balance to estimate the freshwater discharge at the beach face. Analytical calculations were also used to estimate wave set-up and tidally driven saline seepage into the surf zone and were compared to the salinity-based freshwater discharge estimates. Results suggest that average SGD from ojos along the Yucatan Peninsula Caribbean coast is on the order of 308 m3 d-1 m-1 and varies between sampling regions. Higher discharge was observed in the southern regions (568 m3 d-1 m-1) compared to the north (48 m3 d-1 m-1). Discharge at the beach face was in the range of 3.3-8.5 m3 d-1 m-1 for freshwater and 2.7 m3 d-1 m-1 for saline water based on the salinity mass balance and wave- and tidally-driven discharge, respectively. Although discharge from the ojos was larger in volume than discharge from the unconfined aquifer at the beach face, dissolved inorganic nitrogen (DIN) was significantly higher in beach groundwater; thus, discharge of this unconfined beach aquifer groundwater contributed significantly to total DIN loading to the coast. DIN fluxes were up to 9.9 mol d-1 m-1 from ojos and 2.1 mol d-1 m-1 from beach discharge and varied regionally along the 500 km coastline sampled. These results demonstrate the importance of considering the beach zone as a significant nutrient source to coastal waters

  7. Stable Isotope Evidence of Variation in Nitrogen Fixation by Cyanobacteria in Coastal Ecosystems

    NASA Astrophysics Data System (ADS)

    Paul, V.; Clementz, M.

    2006-12-01

    Increased nutrient loading via both natural and anthropogenic factors has been reported as one possible mechanism for the recent increase in the occurrence and intensity of harmful algal blooms (HAB) in coastal ecosystems. Influx of iron, phosphorous, and organic carbon have proven to be significant stimulating factors for HAB, since the benthic cyanobacteria that often make up these blooms are capable of nitrogen-fixation and require these nutrients for this process as well as photosynthesis. These cyanobacteria can switch to direct uptake of dissolved inorganic nitrogen (DIN), however, when concentrations are high enough to energetically favor this source, suggesting that high nitrogen input may also stimulate HAB. Given the distinct isotope differences between atmospheric N2 (0‰) and anthropogenic sources of DIN (>6‰), measurement of the δ15N composition of cyanobacteria can provide a means of gauging the relative significance of anthropogenic versus atmospheric nitrogen to the growth of these blooms. Likewise, the δ13C composition of these primary producers is controlled by the δ13C composition of the DIC, and can be a second tracer of anthropogenic influx into marine ecosystems. A combined approach using both isotope tracers was employed to determine the significance of anthropogenic nitrogen on HAB in subtropical/tropical coastal marine ecosystems. Samples of cyanobacteria and associated macroalgae were collected from three coastal sites in Guam (Facpi Point, Tanguisson, and Ypao Beach), one locality in Hawaii, and three sites in southern Florida (Pepper Park, Fort Lauderdale, Florida Keys). Following removal of marine carbonates via an acid rinse, the δ13C and δ15N values were determined for each species. Cyanobacterial δ15N values ranged from -2.3‰ to 7.7‰ with the highest values reported from sites in Guam. Only cyanobacteria sampled from Hawaii showed no isotope evidence of an anthropogenic source for nitrogen. A strong negative correlation

  8. spatial and temporal distribution of nutrients in a linked stream-lake ecosystem

    NASA Astrophysics Data System (ADS)

    Kalinin, A. V.; Covino, T. P.; McGlynn, B. L.

    2011-12-01

    The movement of nutrients between streams and lakes can impact nutrient export and aquatic ecology in linked stream-lake ecosystems. Specifically, lakes can alter water chemistry and buffer downstream export of nutrients through physical, chemical, and biological processes. This study characterizes nitrogen storage and transport dynamics in a connected stream-lake ecosystem over the summer of 2008 in the Bull Trout Lake Watershed in the Sawtooth Mountains of central Idaho, USA. Water samples were collected for chemical analyses at the lake inflow, outflow, and at six sites across the lake, on hourly to bi-weekly intervals. Lake sampling sites were each sampled at six depths in order to capture all strata of the lake. Additionally, a dye-tracer (Rhodamine-WT) was co-injected with LiCl into the lake to determine water flow-paths and residence time distributions. Inflow and outflow fluxes, spatial and temporal distributions of dissolved organic nitrogen(DON) and dissolved inorganic nitrogen (DIN), as well as water residence times at different lake depths were evaluated. Over the summer of 2008, net influx of NO3 to the lake and net export of DON and NH4 from the lake was observed. While NO3 dominated the DIN fraction at the inflow, NH4 was dominant both at the lake outflow and within the lake, suggesting potential contributions of NH4 to the lake from adjacent wetland and groundwater sources. Differences in transport dynamics between NO3 and NH4, and temporal concentration dynamics both in the stream and lake support this hypothesis. NO3 concentrations were driven by snowmelt flushing and peaked with the hydrograph, subsequently declining for the rest of the summer. NH4 concentrations however remained stable and peaked three weeks after NO3 at the lake outflow, at a time when the contribution of snow melt water had declined and groundwater contribution increased proportionally. In the lake, NH4 and DON concentrations declined during peak runoff in May and June, and

  9. The influence of a lake-wetland complex on catchment nutrient flux and speciation

    NASA Astrophysics Data System (ADS)

    Covino, T. P.; McGlynn, B. L.; Kalinin, A.

    2013-12-01

    Lakes and wetlands within stream networks can substantially alter the timing, magnitude, and form of nutrient export to downstream receiving bodies. These linked systems have the capacity to alter water chemistry and buffer downstream export of nutrients through combined physical, chemical, and biological processes. In many mountainous catchments wetlands are located at the inlet of lakes, thus forming lake-wetland complexes. In this study we investigated the influence an in network lake-wetland complex exerted on the timing, magnitude, and form of carbon and nitrogen export from the Bull Trout Lake Watershed (11.4 km2) located in the Sawtooth Mountains of central Idaho, USA. We: 1) injected conservative tracer to determine lake residence times; and 2) sampled the lake inflow, outflow, and six sites across the lake on hourly to bi-weekly intervals over 5 months (May - September) to capture the spatial and temporal dynamics of injected tracers and catchment nutrient fluxes. Lake sampling sites were each sampled at six depths to capture all strata of the lake. Injected tracer had a median travel time of one week and a modal travel time of four days. Additionally, longer residence times were observed in deeper (>8 m) regions of the lake. We observed that nitrate (NO3-N) was the dominant form of dissolved inorganic nitrogen (DIN) at the lake inflow whereas ammonium (NH4-N) became the dominant component at the lake outflow. Specifically, NO3-N accounted for 62% of DIN at the inflow and NH4-N comprised 58% percent of DIN at the lake outflow 600 m downstream. Dissolved organic nitrogen (DON) comprised the majority of total dissolved nitrogen (TDN) at both the lake inflow and outflow, and within the lake accounting for 92% of the seasonal TDN flux. There was a positive net export of NH4-N, DON, TDN, and dissolved organic carbon (DOC) and a negative net flux of NO3-N from the inlet to the outlet of the lake-wetland complex. Additionally, we observed high concentrations of

  10. Growth of a young pingo in the Canadian Arctic observed by RADARSAT-2 interferometric satellite radar

    NASA Astrophysics Data System (ADS)

    Samsonov, Sergey V.; Lantz, Trevor C.; Kokelj, Steven V.; Zhang, Yu

    2016-04-01

    Advancements in radar technology are increasing our ability to detect Earth surface deformation in permafrost environments. In this paper we use satellite Differential Interferometric Synthetic Aperture Radar (DInSAR) to describe the growth of a large, relatively young pingo in the Tuktoyaktuk Coastlands. High-resolution RADARSAT-2 imagery (2011-2014) analyzed with the Multidimensional Small Baseline Subset (MSBAS) DInSAR revealed a maximum 2.7 cm yr-1 of domed uplift located in a drained lake basin. Satellite measurements suggest that this feature is one of the largest diameter pingos in the region that is presently growing. Observed changes in elevation were modeled as a 348 × 290 m uniformly loaded elliptical plate with clamped edge. Analysis of historical aerial photographs suggested that ground uplift at this location initiated sometime between 1935 and 1951 following drainage of the residual pond. Uplift is largely due to the growth of intrusive ice, because the 9 % expansion of pore water associated with permafrost aggradation into saturated sands is not sufficient to explain the observed short- and long-term deformation rates. The modeled thickness of ice-rich permafrost using the Northern Ecosystem Soil Temperature (NEST) was consistent with the maximum height of this feature. Modeled permafrost aggradation from 1972 to 2014 approximated elevation changes estimated from aerial photographs for that time period. Taken together, these lines of evidence indicate that uplift is at least in part a result of freezing of the sub-pingo water lens. Seasonal variations in the uplift rate seen in the DInSAR data closely match the modeled seasonal pattern in the deepening rate of freezing front. This study demonstrates that interferometric satellite radar can detect and contribute to understanding the dynamics of terrain uplift in response to permafrost aggradation and ground ice development in remote polar environments. The present-day growth rate is smaller than

  11. Effects of Nitrogen Inputs and Watershed Characteristics on Summer Stream Nitrogen Concentrations: A National-Scale Analysis

    NASA Astrophysics Data System (ADS)

    Bellmore, R. A.; Compton, J.; Weber, M.; Hill, R. A.; Thornbrugh, D.; Brooks, J. R.

    2015-12-01

    Nitrogen (N) inputs to the landscape have been linked previously to N loads exported from watersheds at the national scale; however, stream N concentration is arguably more relevant than N load for drinking water quality, freshwater biological responses and establishment of nutrient criteria. In this study, we combine national-scale anthropogenic N input data, including synthetic fertilizer, crop biological N fixation, manure applied to farmland, atmospheric N deposition, and point source inputs, with data from the 2008-09 National Rivers and Streams Assessment to quantify the relationship between N inputs and in-stream concentrations of total N (TN), dissolved inorganic N (DIN), and total organic N (TON) (calculated as TN - DIN). In conjunction with simple linear regression, we use multiple regression to understand how watershed and stream reach attributes modify the effect of N inputs on N concentrations. Median TN was 0.50 mg N L-1 with a maximum of 25.8 mg N L-1. Total N inputs ranged from less than 1 to 196 kg N ha-1 y-1, with a median of 14.4 kg N ha-1 y-1. Atmospheric N deposition was the single largest anthropogenic N source in the majority of sites, but agricultural sources generally dominate total N inputs in sites with elevated N concentrations. The sum of all N inputs were positively correlated with concentrations of all forms of N [r2 = 0.44, 0.43, and 0.18 for TN, DIN, and TON, respectively (all log-transformed), n = 1112], indicating that watershed N inputs are strongly related to stream N concentrations during the summer, despite this being a biologically active and N-retentive period. Additionally, model results suggest that watershed characteristics like wetland area, riparian disturbance and forest cover moderate the effects of watershed N loading on in-stream N concentrations, and different forms of N are likely to respond differently to increasing agricultural and atmospheric N inputs depending on local watershed characteristics.

  12. A time series deformation estimation in the NW Himalayas using SBAS InSAR technique

    NASA Astrophysics Data System (ADS)

    Kumar, V.; Venkataraman, G.

    2012-12-01

    A time series land deformation studies in north western Himalayan region has been presented in this study. Synthetic aperture radar (SAR) interferometry (InSAR) is an important tool for measuring the land displacement caused by different geological processes [1]. Frequent spatial and temporal decorrelation in the Himalayan region is a strong impediment in precise deformation estimation using conventional interferometric SAR approach. In such cases, advanced DInSAR approaches PSInSAR as well as Small base line subset (SBAS) can be used to estimate earth surface deformation. The SBAS technique [2] is a DInSAR approach which uses a twelve or more number of repeat SAR acquisitions in different combinations of a properly chosen data (subsets) for generation of DInSAR interferograms using two pass interferometric approach. Finally it leads to the generation of mean deformation velocity maps and displacement time series. Herein, SBAS algorithm has been used for time series deformation estimation in the NW Himalayan region. ENVISAT ASAR IS2 swath data from 2003 to 2008 have been used for quantifying slow deformation. Himalayan region is a very active tectonic belt and active orogeny play a significant role in land deformation process [3]. Geomorphology in the region is unique and reacts to the climate change adversely bringing with land slides and subsidence. Settlements on the hill slopes are prone to land slides, landslips, rockslides and soil creep. These hazardous features have hampered the over all progress of the region as they obstruct the roads and flow of traffic, break communication, block flowing water in stream and create temporary reservoirs and also bring down lot of soil cover and thus add enormous silt and gravel to the streams. It has been observed that average deformation varies from -30.0 mm/year to 10 mm/year in the NW Himalayan region . References [1] Massonnet, D., Feigl, K.L.,Rossi, M. and Adragna, F. (1994) Radar interferometry mapping of

  13. Soluble reactive phosphorus (SRP) transport and retention in tropical, rain forest streams draining a volcanic landscape in Costa Rica: In situ SRP amendment to streams and laboratory studies

    USGS Publications Warehouse

    Triska, F.; Pringle, C.M.; Duff, J.H.; Avanzino, R.J.; Zellweger, G.

    2006-01-01

    Soluble reactive phosphorus (SRP) transport/retention was determined in two rain forest streams (Salto, Pantano) draining La Selva Biological Station, Costa Rica. There, SRP levels can be naturally high due to groundwater enriched by geothermal activity within the surfically dormant volcanic landscape, and subsequently discharged at ambient temperature. Combined field and laboratory approaches simulated high but natural geothermal SRP input with the objective of estimating the magnitude of amended SRP retention within high and low SRP settings and determining the underlying mechanisms of SRP retention. First, we examined short-term SRP retention/transport using combined SRP-conservative tracer additions at high natural in situ concentrations. Second, we attempted to observe a DIN response during SRP amendment as an indicator of biological uptake. Third, we determined SRP release/retention using laboratory sediment assays under control and biologically inhibited conditions. Short-term in situ tracer-SRP additions indicated retention in both naturally high and low SRP reaches. Retention of added SRP mass in Upper Salto (low SRP) was 17% (7.5 mg-P m-2 h-1), and 20% (10.9 mg-P m-2 h -1) in Lower Salto (high SRP). No DIN response in either nitrate or ammonium was observed. Laboratory assays using fresh Lower Salto sediments indicated SRP release (15.4 ?? 5.9 ??g-P g dry wt.-1 h -1), when incubated in filter sterilized Salto water at ambient P concentration, but retention when incubated in filter sterilized river water amended to 2.0 mg SRP l-1 (233.2 ?? 5.8 ??g-P g dry wt. -1 h-1). SRP uptake/release was similar in both control- and biocide-treated sediments indicating predominantly abiotic retention. High SRP retention even under biologically saturated conditions, absence of a DIN response to amendment, patterns of desorption following amendment, and similar patterns of retention and release under control and biologically inhibited conditions all indicated

  14. Importance of within-lake processes in affecting the dynamics of dissolved organic carbon and dissolved organic and inorganic nitrogen in an Adirondack forested lake/watershed

    NASA Astrophysics Data System (ADS)

    Kang, Phil-Goo; Mitchell, Myron J.; McHale, Patrick J.; Driscoll, Charles T.; Inamdar, Shreeram; Park, Ji-Hyung

    2016-05-01

    Lakes nested in forested watersheds play an important role in mediating the concentrations and fluxes of dissolved organic matter. We compared long-term patterns of concentrations and fluxes of dissolved organic carbon (DOC) and dissolved organic (DON) and inorganic nitrogen (DIN) in aquatic ecosystems of the Arbutus Lake watershed to evaluate how a lake nested in a forested watershed affects the sources (e.g., production) and sinks (e.g., retention) of DOC and DON in the Adirondack Mountains of New York, USA. We observed no significant long-term changes of DOC and DON in the lake outlet since 1983 and 1994, respectively. However, the temporal patterns of DOC and DON concentrations in the lake inlet showed significant seasonality such as increases during the vegetation-growing season along with notable decreases in the dormant season. A comparison of mass balances between inlet and outlet for the period from 2000 to 2009 suggested that the lake was a sink of DOC (mean of influx minus outflux: +1140 mol C ha-1 yr-1). In contrast, the difference of discharge-weighted DON concentrations (mean of inlet minus outlet: -1.0 µmol N L-1) between inlet and outlet was much smaller than the discharge-weighted DOC concentrations (average of inlet minus outlet: + 87 µmol C L-1). DON fluxes showed considerable variation among years (mean of influx minus outflux: +8 mol N ha-1 yr-1; range of differences: -15 to 27 mol N ha-1 yr-1). DON exhibited low percent retention ((influx-outflux)/influx) (mean: 6.9 %, range: -34.8 to +31.2) compared to DOC (mean: 30.1 %, range: +9.2 to +44.1). The resultant increase of DON within the lake was closely linked with a net decrease of DIN through monthly Pearson correlation analysis, suggesting the importance of biotic factors in mediating lake DON dynamics. Our results show different relative retentions of DOC compared with DON, along with a larger retention of DIN than DON, suggesting that DOC and DON might display substantially different

  15. Nitrogen Isotopic Study of Benthic Macroalgae and Seawater in Biscayne Bay, Florida

    NASA Astrophysics