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1

Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry  

Microsoft Academic Search

An approach to the systematic identification and quantification of the proteins contained in the microsomal fraction of cells is described. It consists of three steps: (1) preparation of microsomal fractions from cells or tissues representing different states; (2) covalent tagging of the proteins with isotope-coded affinity tag (ICAT) reagents followed by proteolysis of the combined labeled protein samples; and (3)

David K. Han; Jimmy Eng; Huilin Zhou; Ruedi Aebersold

2001-01-01

2

Protein profiling with cleavable isotope-coded affinity tag (cICAT) reagents: the yeast salinity stress response.  

PubMed

Protein expression profiles in yeast cells, in response to salinity stress, were determined using the cleavable isotope-coded affinity tag (cICAT) labeling strategy. The analysis included separation of the mixed protein samples by SDS-PAGE, followed by excision of the entire gel lane, and division of the lane into 14 gel regions. Regions were subjected to in-gel digestion, biotin affinity chromatography, and analysis by nano-scale microcapillary liquid chromatography coupled to tandem mass spectrometry. The novel (13)C-labeled ICAT reagents have identical elution profiles for labeled peptide pairs and broadly spread the distribution of labeled peptides during reversed-phase chromatography. A total of 560 proteins were identified and quantified, with 51 displaying more than 2-fold expression differences. In addition to some known proteins involved in salt stress, four RNA-binding proteins were found to be up-regulated by high salinity, suggesting that selective RNA export from the nucleus is important for the salt-stress response. Some proteins involved in amino acid synthesis, which have been observed to be up-regulated by amino acid starvation, were also found to increase their abundance on salt stress. These results indicate that salt stress and amino acid starvation cause overlapping cellular responses and are likely to be physiologically linked. PMID:14506205

Li, Jiaxu; Steen, Hanno; Gygi, Steven P

2003-11-01

3

Evaluation of the acid-cleavable isotope-coded affinity tag reagents: application to camptothecin-treated cortical neurons.  

PubMed

The new generation of isotope-coded affinity tag (ICAT) reagents have been evaluated by labeling an equimolar amount of bovine serum albumin (BSA) with ICAT-12C9 and ICAT-13C9, combining the mixtures, digesting them with trypsin and analyzing the digestate both by muRPLC-tandem MS and by matrix-assisted laser desorption ionization (MALDI) TOF/TOF MS. The use of 13C in place of 2H resulted in both of the labeled peptides having identical elution characteristics in a reversed-phase separation. This similarity in elution allows ICAT-labeled peptides to be effectively analyzed using a muRPLC-MALDI-MS strategy as well. All of the cysteinyl-containing tryptic peptides from BSA were identified with only a 10% variation in the relative abundance measurements between the light and heavy versions of each peptide. A facile method for the removal of contaminants that arise from the cleaved biotin moiety that otherwise interfere with downstream separations and MS analysis has also been developed. The new ICAT reagents were then applied to the analysis of a cortical neuron proteome sample to identify proteins regulated by the antitumor drug, camptothecin. PMID:15253428

Yu, Li-Rong; Conrads, Thomas P; Uo, Takuma; Issaq, Haleem J; Morrison, Richard S; Veenstra, Timothy D

2004-01-01

4

Accurate Qualitative and Quantitative Proteomic Analysis of Clinical Hepatocellular Carcinoma Using Laser Capture Microdissection Coupled with Isotope-coded Affinity Tag and Two-dimensional Liquid Chromatography Mass Spectrometry  

Microsoft Academic Search

Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. This study combined the LCM with isotope-coded affinity tag (ICAT) technology and two-di- mensional liquid chromatography to investigate the qual- itative and quantitative proteomes of hepatocellular car- cinoma (HCC). The effects of three

Chen Li; Yi Hong; Ye-Xiong Tan; Hu Zhou; Jian-Hua Ai; Su-Jun Li; Lei Zhang; Qi-Chang Xia; Jia-Rui Wu; Hong-Yang Wang; Rong Zeng

2004-01-01

5

Comprehensive quantitative proteome analysis of 20S proteasome subtypes from rat liver by isotope coded affinity tag and 2-D gel-based approaches.  

PubMed

Quantitative protein profiling is an essential part of proteomics and requires technologies that accurately, reproducibly, and comprehensively identify and quantify proteins. Over the past years, many quantitative proteomic methods have been developed. Here, 20S proteasome subtypes isolated from rat were compared by four approaches based on the combination of isotope-coded affinity tag (ICAT), 2-DE, LC and ESI and MALDI MS: (i) 2-DE, (ii) ICAT/2-DE MALDI-MS, (iii) ICAT/LC-ESI-MS, (iv) ICAT/LC-MALDI-MS. A definite qualitative advantage of 2-DE gels was the separation of all known protein species, the identification of cysteine sulfoxide of alpha-4 (RC6-IS) and N-terminal acetylation of several subunits. Furthermore, quantitative differences between the standard subunits beta-2, and beta-5 and their immunosubunits were only detected by 2-DE image analysis revealing a higher replacement of standard- by immuno-beta-subunits in subtype IV. It was obvious that for relative quantification only protein spot and mass peaks with a certain level of intensity displayed acceptable values of SD. However, ICAT in conjunction with LC/MALDI-MS was the most accurate method for quantification. The experimental data of this investigation are accessible via http://www.mpiib-berlin.mpg.de/2D-PAGE/. PMID:16858736

Schmidt, Frank; Dahlmann, Burkhardt; Janek, Katharina; Kloss, Alexander; Wacker, Maik; Ackermann, Renate; Thiede, Bernd; Jungblut, Peter R

2006-08-01

6

Analysis of aromatic catabolic pathways in Pseudomonas putida KT 2440 using a combined proteomic approach: 2-DE/MS and cleavable isotope-coded affinity tag analysis.  

PubMed

Proteomic analysis of Pseudomonas putida KT2440 cultured in monocyclic aromatic compounds was performed using 2-DE/MS and cleavable isotope-coded affinity tag (ICAT) to determine whether proteins involved in aromatic compound degradation pathways were altered as predicted by genomic analysis (Jiménez et al., Environ Microbiol. 2002, 4, 824-841). Eighty unique proteins were identified by 2-DE/MS or MS/MS analysis from P. putida KT2440 cultured in the presence of six different organic compounds. Benzoate dioxygenase (BenA, BenD) and catechol 1,2-dioxygenase (CatA) were induced by benzoate. Protocatechuate 3,4-dixoygenase (PcaGH) was induced by p-hydroxybenzoate and vanilline. beta-Ketoadipyl CoA thiolase (PcaF) and 3-oxoadipate enol-lactone hydrolase (PcaD) were induced by benzoate, p-hydroxybenzoate and vanilline, suggesting that benzoate, p-hydroxybenzoate and vanilline were degraded by different dioxygenases and then converged in the same beta-ketoadipate degradation pathway. An additional 110 proteins, including 19 proteins from 2-DE analysis, were identified by cleavable ICAT analysis for benzoate-induced proteomes, which complemented the 2-DE results. Phenylethylamine exposure induced beta-ketoacyl CoA thiolase (PhaD) and ring-opening enzyme (PhaL), both enzymes of the phenylacetate (pha) biodegradation pathway. Phenylalanine induced 4-hydroxyphenyl-pyruvate dioxygenase (Hpd) and homogentisate 1,2-dioxygenase (HmgA), key enzymes in the homogentisate degradation pathway. Alkyl hydroperoxide reductase (AphC) was induced under all aromatic compounds conditions. These results suggest that proteome analysis complements and supports predictive information obtained by genomic sequence analysis. PMID:16470664

Kim, Young Hwan; Cho, Kun; Yun, Sung-Ho; Kim, Jin Young; Kwon, Kyung-Hoon; Yoo, Jong Shin; Kim, Seung Il

2006-02-01

7

Comparative Glycomics using A Tetraplex Stable-Isotope Coded Tag  

PubMed Central

This study illustrates the utility of tetraplex stable isotope coded tags in mass spectrometric glycomics using three carbohydrate classes. The teteraplex tags allow for the direct comparison of glycan compositions within four samples using capillary scale hydrophilic interaction chromatography with on-line mass spectrometry. In addition, the ability to discern glycan structural isomers is shown based on the tandem mass spectra of each composition using nanospray ionization. Results are shown for chondroitin sulfate proteoglycans, low molecular weight heparins, full length heparins, and N-glycans from ?-1-acid glycoproteins from four mammalian species. The data demonstrate the value of the tetraplex stable isotope tagging approach for producing high quality glycomics compositional profiling and fine structural analysis. PMID:20230064

Bowman, Michael J.; Zaia, Joseph

2010-01-01

8

The Application of New Software Tools to Quantitative Protein Profiling Via Isotope-coded Affinity Tag (ICAT) and Tandem Mass Spectrometry I. STATISTICALLY ANNOTATED DATASETS FOR PEPTIDE SEQUENCES AND PROTEINS IDENTIFIED VIA THE APPLICATION OF ICAT AND TANDEM MASS SPECTROMETRY TO PROTEINS COPURIFYING WITH T CELL LIPID RAFTS*? S  

Microsoft Academic Search

Lipid rafts were prepared according to standard protocols from Jurkat T cells stimulated via T cell receptor\\/CD28 cross-linking and from control (unstimulated) cells. Co- isolating proteins from the control and stimulated cell preparations were labeled with isotopically normal (d0) and heavy (d8) versions of the same isotope-coded affinity tag (ICAT) reagent, respectively. Samples were combined, proteolyzed, and resultant peptides fractionated

Priska D. von Haller; Eugene Yi; Samuel Donohoe; Kelly Vaughn; Andrew Keller; Alexey I. Nesvizhskii; Jimmy Eng; Xiao-jun Li; David R. Goodlett; Ruedi Aebersold; Julian D. Watts

9

Phosphoprotein Isotope-Coded Solid-Phase Tag Approach for Enrichment and Quantitative Analysis of Phosphopeptides from Complex Mixtures  

SciTech Connect

Many cellular processes are regulated by reversible protein phosphorylation and the ability to identify and quantify phosphoproteins from proteomes is essential for gaining a better understanding of these dynamic cellular processes. However, a sensitive, efficient and global method capable of addressing the phosphoproteome has yet to be developed. Here we describe an improved stable-isotope labeling method using a Phosphoprotein Isotope-coded Solid-phase Tag (PhIST) for isolating and measuring the relative abundance of phosphorylated peptides from complex peptide mixtures resulting from the enzymatic digestion of extracted proteins. The PhIST approach is an extension of the previously reported Phosphoprotein Isotope-coded Affinity Tag (PhIAT)approach developed by our laboratory1-2, where the O-phosphate moiety on phosphoseryl or phosphothreonyl residues were derivatized by hydroxide ion-medated B-elimination followed by the addition of 1,2-ethanedithiol (EDT). Instead of using the biotin affinity tag, peptides containing the EDT moiety were captured and labeled in one step using isotope-coded solid-phase reagents containing either light (12C6, 14N) or heavy (13C6, 15N) stable isotopes. The captured peptides labeled with the isotope-coded tags were released from the solid-phase support by UV photocleavage and analyzed by capillary LC-MS/MS. The efficiency and sensitivity of the PhIST labeling approach for identification of phosphopeptides from mixtures was demonstrated using casein phosphoproteins. Its utility for proteomic applications is demonstrated by the labeling of soluble proteins from human breast cancer cell line.

Qian, Weijun (BATTELLE (PACIFIC NW LAB)) [BATTELLE (PACIFIC NW LAB); Goshe, Michael B.(North Carolina State University) [North Carolina State University; Camp, David G.(BATTELLE (PACIFIC NW LAB)) [BATTELLE (PACIFIC NW LAB); Yu, Li-Rong (BATTELLE (PACIFIC NW LAB)) [BATTELLE (PACIFIC NW LAB); Tang, Keqi (BATTELLE (PACIFIC NW LAB)) [BATTELLE (PACIFIC NW LAB); Smith, Richard D.(BATTELLE (PACIFIC NW LAB)) [BATTELLE (PACIFIC NW LAB)

2003-10-15

10

Combining protein identification and quantification: C-terminal isotope-coded tagging using sulfanilic acid.  

PubMed

Two methods of differential isotopic coding of carboxylic groups have been developed to date. The first approach uses d0- or d3-methanol to convert carboxyl groups into the corresponding methyl esters. The second relies on the incorporation of two 18O atoms into the C-terminal carboxylic group during tryptic digestion of proteins in H(2)18O. However, both methods have limitations such as chromatographic separation of 1H and 2H derivatives or overlap of isotopic distributions of light and heavy forms due to small mass shifts. Here we present a new tagging approach based on the specific incorporation of sulfanilic acid into carboxylic groups. The reagent was synthesized in a heavy form (13C phenyl ring), showing no chromatographic shift and an optimal isotopic separation with a 6 Da mass shift. Moreover, sulfanilic acid allows for simplified fragmentation in matrix-assisted laser desorption/ionization (MALDI) due the charge fixation of the sulfonate group at the C-terminus of the peptide. The derivatization is simple, specific and minimizes the number of sample treatment steps that can strongly alter the sample composition. The quantification is reproducible within an order of magnitude and can be analyzed either by electrospray ionization (ESI) or MALDI. Finally, the method is able to specifically identify the C-terminal peptide of a protein by using GluC as the proteolytic enzyme. PMID:16628568

Panchaud, Alexandre; Guillaume, Elisabeth; Affolter, Michael; Robert, Fabien; Moreillon, Philippe; Kussmann, Martin

2006-01-01

11

Mapping peptide thiol accessibility in membranes using a quaternary ammonium isotope-coded mass tag (ICMT)  

PubMed Central

The plasma membrane contains a diverse array of proteins, including receptors, channels, and signaling complexes, that serve as decision-making centers. Investigation of membrane protein topology is important for understanding the function of these types of protein. Here, we report a method to determine protein topology in the membrane that utilizes labeling of cysteine with isotope-coded mass tags. The mass tags contain a thiol reactive moiety, linker, and a quaternary ammonium group to aid ionization in the mass spectrometer and were synthesizes as both light and heavy (deuterated) forms. The probes were found to be membrane impermeable when applied to lipid vesicles. To assess the utility of the probes for mapping peptide thiol topology, we employed a two-step labeling procedure. Vesicles containing ?-helical transmembrane peptides were labeled with heavy (or light) probe, solubilized by detergent, and then labeled by an excess of the complementary probe. Peptide for which the cysteine was oriented in the center of the lipid bilayer was not labeled until the lipid vesicles were lysed with detergent, consistent with the membrane impermeability of the probes and reduced ionization of the thiol in the hydrophobic membrane. Peptide for which the cysteine was positioned in the head group zone of the lipid bilayer was labeled rapidly. Peptide for which the cysteine was positioned below the head group abutting the hydrocarbon region was labeled at a reduced rate compared to the fully accessible cysteine. Moreover, the effect of lipid bilayer structure on the kinetics of peptide and lipid flipping in the bilayer was readily measured with our two-step labeling method. The small sample size required, the ease and rapidity of sample preparation, and the amenability of MALDI-TOF mass spectral to analysis in the presence of lipids will enable future facile investigation of membrane proteins in a cellular context. PMID:23725486

Su, Chiao-Yung; London, Erwin; Sampson, Nicole S.

2013-01-01

12

Affinity Chromatography GST-tagged Proteins  

E-print Network

Affinity Chromatography GST-tagged Proteins His-tagged Proteins Antibody Immobilization Nucleotide binding Proteins Phospho-Aminoacid binding Proteins www.jenabioscience.com #12;Table of Contents AffinityChromatography Affinity Chromatography 3 GST-tagged Proteins 4 Glutathione ChroMatrixTM, Fast Flow 4 GST Cleavage Capture

Lebendiker, Mario

13

Quantification of tryptic peptides in quadrupole ion trap using high-mass signals derived from isotope-coded N-acetyl dipeptide tags.  

PubMed

Isotope-labeled N-acetyl dipeptides (Ac-Xxx-Ala) are coupled to the primary amines of tryptic peptides and then analyzed by tandem mass spectrometry. Amide bond cleavage between Xxx and Ala provides both low- and high-mass isotope-coded signals for quantification of peptides. Especially, facile cleavage at the modified lysine side chain yields very strong high-mass quantitation signals in a noise-free region. Tagging tryptic peptides with isobaric N-acetyl dipeptides is a viable strategy for accurate quantification of proteins, which can be used with most quadrupole ion trap mass spectrometers carrying the 1/3 mass cut-off problem. PMID:21953270

Seo, Jongcheol; Yoon, Hye-Joo; Shin, Seung Koo

2011-09-01

14

A tailor-made "tag-receptor" affinity pair for the purification of fusion proteins.  

PubMed

A novel affinity "tag-receptor" pair was developed as a generic platform for the purification of fusion proteins. The hexapeptide RKRKRK was selected as the affinity tag and fused to green fluorescent protein (GFP). The DNA fragments were designed, cloned in Pet-21c expression vector and expressed in E. coli host as soluble protein. A solid-phase combinatorial library based on the Ugi reaction was synthesized: 64 affinity ligands displaying complementary functionalities towards the designed tag. The library was screened by affinity chromatography in a 96-well format for binding to the RKRKRK-tagged GFP protein. Lead ligand A7C1 was selected for the purification of RKRKRK fusion proteins. The affinity pair RKRKRK-tagged GFP with A7C1 emerged as a promising solution (Ka of 2.45×10(5) ?M(-1) ). The specificity of the ligand towards the tag was observed experimentally and theoretically through automated docking and molecular dynamics simulations. PMID:24903894

Pina, Ana S; Guilherme, Márcia; Pereira, Alice S; Fernandes, Cláudia S F M; Branco, Ricardo J F; El Khoury, Graziella; Lowe, Christopher R; Roque, A Cecília A

2014-07-01

15

CABM Symposium Differential effects of supplementary affinity tags on the solubility of MBP  

E-print Network

fusion partners, Escherichia coli maltose-binding protein (MBP) is a particularly useful affinity tag, isopropyl- -D- thiogalactopyranoside; MBP, E. coli maltose-binding protein; GFP; green fluorescent protein their solubility [1]. Among the affinity tags that have been character- ized to date [2], only E. coli MBP is also

16

Purification of a recombinant protein with cellulose-binding module 3 as the affinity tag.  

PubMed

Easy-to-perform and low-cost protein purification methods are in high demand for the mass production of commonly used enzymes that play an important role in bioeconomy. A low-cost and rapid recombinant protein purification system was developed using CBM3 (family 3 cellulose-binding module) as affinity tag. This protocol describes the purification of CBM3-fusion protein and tag-free protein expressed in Pichia pastoris using CBM3 as an affinity tag. PMID:24943312

Wang, Dongmei; Hong, Jiong

2014-01-01

17

Design of affinity tags for one-step protein purification from immobilized zinc columns  

Microsoft Academic Search

Affinity tags are often used to accomplish recombinant protein purification using immobilized metal affinity chromatography. Success of the tag depends on the chelated metal used and the elution profile of the host cell proteins. Zn(II)-iminodiacetic acid (Zn(II)-IDA) may prove to e superior to either immobilized copper or nickel as a result of its relatively low binding affinity for cellular proteins.

R. S. Pasquinelli; R. E. Shepherd; R. R. Koepsel; A. Zhao; M. M. Ataai

2000-01-01

18

Novel affinity tag system using structurally defined antibody-tag interaction: Application to single-step protein purification  

PubMed Central

Biologically important human proteins often require mammalian cell expression for structural studies, presenting technical and economical problems in the production/purification processes. We introduce a novel affinity peptide tagging system that uses a low affinity anti-peptide monoclonal antibody. Concatenation of the short recognition sequence enabled the successful engineering of an 18-residue affinity tag with ideal solution binding kinetics, providing a low-cost purification means when combined with nondenaturing elution by water-miscible organic solvents. Three-dimensional information provides a firm structural basis for the antibody–peptide interaction, opening opportunities for further improvements/modifications. PMID:18787202

Nogi, Terukazu; Sangawa, Takeshi; Tabata, Sanae; Nagae, Masamichi; Tamura-Kawakami, Keiko; Beppu, Ayako; Hattori, Mitsuharu; Yasui, Norihisa; Takagi, Junichi

2008-01-01

19

Isotope-coded protein label.  

PubMed

A great variety of technologies using stable isotope labeling in combination with mass spectrometry have been described being tools to identify and relatively quantify proteins within complex mixtures. Here, we present a method, termed isotope-coded protein label (ICPL), which is capable of high-throughput quantitative proteome profiling on a global scale. Since ICPL is based on tagging stable isotope derivatives at the free amino groups of intact proteins, the method is applicable to any protein sample, including extracts from tissues or body fluids. All separation methods currently employed in proteome studies can be used to reduce complexity on the protein level. After enzymatic cleavage of the protein fractions, the ratios of peptides from different proteome states can be calculated by simple MS-based mass spectrometric analyses. Only peptides representing different expression levels in the different proteomic states are further analyzed by tandem-mass spectrometry to identify respective proteins. For quantification of proteins from multiplexed ICPL experiments, ICPLQuant was developed, a software package especially designed to cover the whole ICPL workflow. The ICPL method results in accurate and reproducible quantification of proteins and high sequence coverage, indispensable for a comprehensive detection of posttranslational modifications and discrimination of protein isoforms. PMID:22665300

Kellermann, Josef; Lottspeich, Friedrich

2012-01-01

20

PROTEIN-PROTEIN INTERACTIONS OF TANDEM AFFINITY PURIFICATION (TAP)-TAGGED PROTEIN KINASES IN RICE  

Technology Transfer Automated Retrieval System (TEKTRAN)

Forty-one rice cDNAs encoding protein kinases were fused to the tandem affinity purification (TAP) tag and expressed in transgenic rice plants. The TAP-tagged kinases and interacting proteins were purified from the T1 progeny of the transgenic rice plants and identified by mass spectrometry. Ninet...

21

Dual-tagging system for the affinity purification of mammalian protein complexes  

SciTech Connect

Although affinity purification coupled with mass spectrometry (MS) provides a powerful tool to study protein-protein interactions, this strategy has encountered numerous difficulties when adapted to mammalian cells. Here we describe a Gateway{reg_sign}-compatible dual-tag affinity purification system that integrates regulatable expression, tetracysteine motifs, and various combinations of affinity tags to facilitate the cloning, detection, and purification of bait proteins and their interacting partners. Utilizing the human telomere binding protein TRF2 as a benchmark, we demonstrate bait protein recoveries upwards of approximately 16% from as little as 1-7 x 10{sup 7} cells and successfully identify known TRF2 interacting proteins, suggesting that our dual-tag affinity purification approach is a capable new tool for expanding the capacity to explore mammalian proteomic networks.

Giannone, Richard J [ORNL; McDonald, W Hayes [ORNL; Hurst, Gregory {Greg} B [ORNL; Huang, Ying [ORNL; Wu, Jun [ORNL; Liu, Yie [National Institute on Aging, Baltimore; Wang, Yisong [ORNL

2007-01-01

22

Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation.  

PubMed

While antibodies currently play a dominant role as affinity reagents in biological research and for diagnostics, a broad range of recombinant proteins are emerging as promising alternative affinity reagents in detection assays and quantification. DNA-mediated affinity-based assays, such as immuno-PCR and proximity ligation assays (PLA), use oligonucleotides attached to affinity reagents as reporter molecules. Conjugation of oligonucleotides to affinity reagents generally employs chemistries that target primary amines or cysteines. Because of the random nature of these processes neither the number of oligonucleotides conjugated per molecule nor their sites of attachment can be accurately controlled for affinity reagents with several available amines and cysteines. Here, we present a straightforward and convenient approach to functionalize recombinant affinity reagents for PLA by expressing the reagents as fusion partners with SNAP protein tags. This allowed us to conjugate oligonucleotides in a site-specific fashion, yielding precisely one oligonucleotide per affinity reagent. We demonstrate this method using designed ankyrin repeat proteins (DARPins) recognizing the tumor antigen HER2 and we apply the conjugates in different assay formats. We also show that SNAP or CLIP tags, expressed as fusion partners of transfected genes, allow oligonucleotide conjugations to be performed in fixed cells, with no need for specific affinity reagents. The approach is used to demonstrate induced interactions between the fusion proteins FKBP and FRB by allowing the in situ conjugated oligonucleotides to direct the production of templates for localized rolling circle amplification reactions. PMID:22664266

Gu, Gucci Jijuan; Friedman, Mikaela; Jost, Christian; Johnsson, Kai; Kamali-Moghaddam, Masood; Plückthun, Andreas; Landegren, Ulf; Söderberg, Ola

2013-01-25

23

Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification  

Microsoft Academic Search

BACKGROUND: PhaR which is a repressor protein for microbial polyhydroxyalkanoates (PHA) biosynthesis, is able to attach to bacterial PHA granules in vivo, was developed as an affinity tag for in vitro protein purification. Fusion of PhaR-tagged self-cleavable Ssp DnaB intein to the N-terminus of a target protein allowed protein purification with a pH and temperature shift. During the process, the

Shuang Zhang; Zhi Hui Wang; Guo Qiang Chen

2010-01-01

24

Crystallographic structure of Ni-Co coating on the affinity adsorption of histidine-tagged protein.  

PubMed

The principle of immobilized metal affinity chromatography (IMAC) has been recently implemented for protein microarrays for the study of protein abundance and function. Ni-Co film fabricated by electrodeposition is a novel microarray surface in an alloy type for immobilizing histidine-tagged proteins based on IMAC. In this paper, the effects of crystallographic structures and surface properties of Ni-Co coatings, with and without the annealing process, on the immobilization of histidine-tagged proteins were systematically investigated. The experimental results reveal that the stronger hcp texture, due to a higher Co content, results in better affinity adsorption for histidine-tagged biotin. Nevertheless, the allotropic phase transformation from hcp to fcc, due to the annealing process, leads to the decrease of affinity adsorption. The wettability property and the surface roughness of Ni-Co coating are, however, not important factors. Obviously, the crystallographic structure of Ni-Co coating is the dominant factor for the specific affinity adsorption of histidine-tagged protein. PMID:25731093

Chang, Yaw-Jen; Chen, Sheng-Zheng; Ho, Ching-Yuan

2015-04-01

25

Yeast 3',5'-bisphosphate nucleotidase: an affinity tag for protein purification.  

PubMed

Affinity chromatography is one of the most popular methods for protein purification. Each tag method has its advantages and disadvantages, and combination of different tags and developing of new tags had been proposed and performed. Yeast 3',5'-bisphosphate nucleotidase, also known as HAL2, hydrolyzes 3'-phosphoadenosine 5'-phosphate (PAP) with submicromolar Km, which indicated the tight interactions between HAL2 and PAP. In order to explore the feasibility of HAL2 as a protein purification affinity tag, HAL2 was further characterized with PAP as substrate. Results demonstrated that KmPAP and kcatPAP were ?0.3?M and ?11s(-)(1), respectively. Kd for PAP was 0.008?M in the presence of Ca(2+). pH was also found to affect interactions between HAL2 and PAP, with tightest binding (Kd?8nM) at pH 7.5 and 8. The purification protocol was rationally designed based on nanomolar affinity to PAP agarose in the presence of Ca(2+), which could satisfy the metal requirement for PAP binding, prevent hydrolysis of immobilized PAP and could be chelated by ethylene glycol tetraacetic acid (EGTA) for elution. A series of expression vectors were further constructed and Escherichia coli adenosine 5'-phosphosulfate kinase (APSK) was prokaryotically expressed, purified and characterized. Ready to use expression vector with eight commonly used restriction enzyme recognition sites in multiple cloning site was subsequently constructed. By comparing with current popular tags, HAL2 was found to be an efficient and economical tag for prokaryotic protein expression and purification. PMID:24613729

Yang, Yang; Ma, Jianhui; Yang, Yilin; Zhang, Xiao; Wang, Yanxing; Yang, Ling; Sun, Meihao

2014-05-01

26

Griffith 4/2004 Small Scale His Tag Enzyme Purification with TALON Affinity Column Resin  

E-print Network

.0 Elution Buffer: 50 mM NaH2PO4 300 mM NaCl 250 mM imidazole pH 7.0 Procedure: 1. Grow E. coli expressing: This is a small scale method for purifying a His-tagged protein using commercial affinity resin. Materials: TALON the protein of interest at 37ºC to an OD600 = 0.5 2. Let cells cool to RT (with shaking) for 1 hour

Doering, Tamara

27

Generation of Affinity-Tagged Fluoromycobacteriophages by Mixed Assembly of Phage Capsids  

PubMed Central

Addition of affinity tags to bacteriophage particles facilitates a variety of applications, including vaccine construction and diagnosis of bacterial infections. Addition of tags to phage capsids is desirable, as modification of the tails can lead to poor adsorption and loss of infectivity. Although tags can readily be included as fusions to head decoration proteins, many phages do not have decoration proteins as virion components. The addition of a small (10-amino-acid) Strep-tag II (STAG II) to the mycobacteriophage TM4 capsid subunit, gp9, was not tolerated as a genetically homogenous recombinant phage but could be incorporated into the head by growth of wild-type phage on a host expressing the capsid-STAG fusion. Particles with capsids composed of wild-type and STAG-tagged subunit mixtures could be grown to high titers, showed good infectivities, and could be used to isolate phage-bacterium complexes. Preparation of a STAG-labeled fluoromycobacteriophage enabled capture of bacterial complexes and identification of infected bacteria by fluorescence. PMID:23851082

Piuri, Mariana; Rondón, Liliana; Urdániz, Estefanía

2013-01-01

28

RNA complex purification using high-affinity fluorescent RNA aptamer tags.  

PubMed

RNA plays important roles in cellular processes, but RNA-protein complexes are notoriously hard to isolate and study. We compare and contrast existing RNA- and protein-purification strategies with the potential of new RNA-tagging systems such as RNA Spinach and RNA Mango. Each RNA aptamer binds a small fluorophore, resulting in a highly fluorescent complex that is thousands of times brighter than the unbound fluorophore. Provided that the aptamer binding affinity is high enough, derivatized dyes can be used in conjunction with these aptamers to purify RNA complexes while simultaneously using their intrinsic fluorescence to track the complex of interest. The known strengths and weakness of these RNA tagging systems are discussed. PMID:25585661

Panchapakesan, Shanker Shyam S; Jeng, Sunny C Y; Unrau, Peter J

2015-04-01

29

Application of coupled affinity-sizing chromatography for the detection of proteolyzed HSA-tagged proteins.  

PubMed

Coupled affinity liquid chromatography and size exclusion chromatography (ALC-SEC) is a technique that has been shown to successfully report product quality of proteins during cell expression and prior to the commencement of downstream processing chromatography steps. This method was applied to monitoring the degradation and subsequent partial remediation of a HSA-tagged protein which showed proteolysis, allowing for rapid cell line development to address this product quality dilemma. This paper outlines the novel application of this method for measuring and addressing protease-induced proteolysis. PMID:25514202

London, Anne Serdakowski; Patel, Kunal; Quinn, Lisa; Lemmerer, Martin

2015-04-01

30

Application of Ni(II)-Assisted Peptide Bond Hydrolysis to Non-Enzymatic Affinity Tag Removal  

PubMed Central

In this study, we demonstrate a non-enzymatic method for hydrolytic peptide bond cleavage, applied to the removal of an affinity tag from a recombinant fusion protein, SPI2-SRHWAP-His6. This method is based on a highly specific Ni(II) reaction with (S/T)XHZ peptide sequences. It can be applied for the protein attached to an affinity column or to the unbound protein in solution. We studied the effect of pH, temperature and Ni(II) concentration on the efficacy of cleavage and developed an analytical protocol, which provides active protein with a 90% yield and ?100% purity. The method works well in the presence of non-ionic detergents, DTT and GuHCl, therefore providing a viable alternative for currently used techniques. PMID:22574150

Kopera, Edyta; Belczyk-Ciesielska, Agnieszka; Bal, Wojciech

2012-01-01

31

Robotic high-throughput purification of affinity-tagged recombinant proteins.  

PubMed

Affinity purification of recombinant proteins has become the method of choice to obtain good quantities and qualities of proteins for a variety of downstream biochemical applications. While manual or FPLC-assisted purification techniques are generally time-consuming and labor-intensive, the advent of high-throughput technologies and liquid handling robotics has simplified and accelerated this process significantly. Additionally, without the human factor as a potential source of error, automated purification protocols allow for the generation of large numbers of proteins simultaneously and under directly comparable conditions. The delivered material is ideal for activity comparisons of different variants of the same protein. Here, we present our strategy for the simultaneous purification of up to 24 affinity-tagged proteins for activity measurements in biochemical assays. The protocol described is suitable for the scale typically required in individual research laboratories. PMID:25749949

Wiesler, Simone C; Weinzierl, Robert O J

2015-01-01

32

Quantitative Proteomics Isotope Coding Proteomics  

E-print Network

Smaller final tag ­ more informative MS/MS spectra Coefficient-of-variation in test system ­ 10% #12 side chains) - isomeric/isobaric labels (MS/MS based readout) Reporter Balance PRG Peptide 145 amu Facilitates quantitation of PTMs #12;control test 1 test 2 test 3 reduce, alkylate, digest label combine

Richardson, David

33

Direct electron transfer to a metagenome-derived laccase fused to affinity tags near the electroactive copper site.  

PubMed

We demonstrate the efficient direct electron transfer (DET) from an electrode to an engineered laccase isolated from a metagenome. The enzyme has a unique homotrimeric architecture with a two-domain-type laccase subunit. The recombinant laccase-modified mesoporous carbon electrode exhibits an effective catalytic current for oxygen reduction, which depends on the affinity tags attached near the electroactive Cu site of the enzyme. We also investigated the effect of the affinity tags on the orientation of the enzyme on functional thiol-modified Au electrodes. The results suggest that a poly-histidine tag (His-tag) functions as an anchor to control the orientation of the enzyme to enhance the current density of the DET-type bioelectrocatalysis. PMID:24185896

Tsujimura, Seiya; Asahi, Masafumi; Goda-Tsutsumi, Maiko; Shirai, Osamu; Kano, Kenji; Miyazaki, Kentaro

2013-12-21

34

Immobilized palladium(II) ion affinity chromatography for recovery of recombinant proteins with peptide tags containing histidine and cysteine.  

PubMed

Fusion of peptide-based tags to recombinant proteins is currently one of the most used tools for protein production. Also, immobilized metal ion affinity chromatography (IMAC) has a huge application in protein purification, especially in research labs. The combination of expression systems of recombinant tagged proteins with this robust chromatographic system has become an efficient and rapid tool to produce milligram-range amounts of proteins. IMAC-Ni(II) columns have become the natural partners of 6xHis-tagged proteins. The Ni(II) ion is considered as the best compromise of selectivity and affinity for purification of a recombinant His-tagged protein. The palladium(II) ion is also able to bind to side chains of amino acids and form ternary complexes with iminodiacetic acid and free amino acids and other sulfur-containing molecules. In this work, we evaluated two different cysteine- and histidine-containing six amino acid tags linked to the N-terminal group of green fluorescent protein (GFP) and studied the adsorption and elution conditions using novel eluents. Both cysteine-containing tagged GFPs were able to bind to IMAC-Pd(II) matrices and eluted successfully using a low concentration of thiourea solution. The IMAC-Ni(II) system reaches less than 20% recovery of the cysteine-containing tagged GFP from a crude homogenate of recombinant Escherichia coli, meanwhile the IMAC-Pd(II) yields a recovery of 45% with a purification factor of 13. Copyright © 2014 John Wiley & Sons, Ltd. PMID:25277090

Kikot, Pamela; Polat, Aise; Achilli, Estefania; Fernandez Lahore, Marcelo; Grasselli, Mariano

2014-11-01

35

Cancer cell sensing and therapy using affinity tag-conjugated gold nanorods  

PubMed Central

Through the developments in controlling the shape of gold nanoparticles, synthesis of gold nanorods (AuNRs) can be considered as a milestone discovery in the area of nanomaterial-based cancer treatments. Besides having tuneable absorption maxima at near infrared (NIR) range, AuNRs have superior absorption cross section at NIR frequencies compared with other gold nanoparticles. When this unique optical property is combined with the specificity against cancer cells used by affinity tag conjugations, AuNRs become one of the most important nanoparticles used in both cancer cell sensing and in therapy. In this review, the impact of size and shape control of nanoparticles, especially AuNRs, on cancer cell treatments and a range of aptamer-conjugated AuNR applications in this regard are reviewed. PMID:24427543

Yasun, Emir; Kang, Huaizhi; Erdal, Huseyin; Cansiz, Sena; Ocsoy, Ismail; Huang, Yu-Fen; Tan, Weihong

2013-01-01

36

Purification of histidine-tagged nucleocapsid protein of Nipah virus using immobilized metal affinity chromatography.  

PubMed

Nucleocapsid (N) protein of Nipah virus (NiV) is a potential serological marker used in the diagnosis of NiV infections. In this study, a rapid and efficient purification system, HisTrap 6 Fast Flow packed bed column was applied to purify recombinant histidine-tagged N protein of NiV from clarified feedstock. The optimizations of binding and elution conditions of N protein of NiV onto and from Nickel Sepharose 6 Fast Flow were investigated. The optimal binding was achieved at pH 7.5, superficial velocity of 1.25 cm/min. The bound N protein was successfully recovered by a stepwise elution with different concentration of imidazole (50, 150, 300 and 500 mM). The N protein of NiV was captured and eluted from an inlet N protein concentration of 0.4 mg/ml in a scale-up immobilized metal affinity chromatography (IMAC) packed bed column of Nickel Sepharose 6 Fast Flow with the optimized condition obtained from the method scouting. The purification of histidine-tagged N protein using IMAC packed bed column has resulted a 68.3% yield and a purification factor of 7.94. PMID:19395325

Chong, Fui Chin; Tan, Wen Siang; Biak, Dayang Radiah Awang; Ling, Tau Chuan; Tey, Beng Ti

2009-05-15

37

Description T7Tag Affinity Purification KIt 69025-3 The T7Tag Affinity Purification Kit is designed for rapid immunoaffinity purification of  

E-print Network

X T7·Tag Neutralization Buffer (2M Tris base pH 10.4) · 1 chromatography column with closures.e. the initial 11aa of the T7 gene 10 protein). Purification is based on binding target proteins to T7·Tag-20, 0.02% sodium azide, pH 7.3) · 20ml 10X T7·Tag Elute Buffer (10X = 1M citric acid pH 2.2) · 20ml 1

Lebendiker, Mario

38

Probing the Mechanism of Electron Capture and Electron Transfer Dissociation Using Tags with Variable Electron Affinity  

PubMed Central

Electron capture dissociation (ECD) and electron transfer dissociation (ETD) of doubly protonated electron affinity (EA)-tuned peptides were studied to further illuminate the mechanism of these processes. The model peptide FQpSEEQQQTEDELQDK, containing a phosphoserine residue, was converted to EA-tuned peptides via ?-elimination and Michael addition of various thiol compounds. These include propanyl, benzyl, 4-cyanobenzyl, perfluorobenzyl, 3,5-dicyanobenzyl, 3-nitrobenzyl and 3,5-dinitrobenzyl structural moieties, having a range of EA from -1.15 to 1.65 eV, excluding the propanyl group. Typical ECD or ETD backbone fragmentations are completely inhibited in peptides with substituent tags having EA over 1.00 eV, which are referred to as electron predators in this work. Nearly identical rates of electron capture by the dications substituted by the benzyl (EA = -1.15 eV) and 3-nitrobenzyl (EA = 1.00 eV) moieties are observed, which indicates the similarity of electron capture cross sections for the two derivatized peptides. This observation leads to the inference that electron capture kinetics are governed by the long range electron-dication interaction and are not affected by side chain derivatives with positive EA. Once an electron is captured to high-n Rydberg states, however, through-space or through-bond electron transfer to the EA-tuning tags or low-n Rydberg states via potential curve crossing occurs in competition with transfer to the amide ?* orbital. The energetics of these processes are evaluated using time-dependent density functional theory with a series of reduced model systems. The intramolecular electron transfer process is modulated by structure-dependent hydrogen bonds and is heavily affected by the presence and type of electron withdrawing groups in the EA-tuning tag. The anion radicals formed by electron predators have high proton affinities (approximately 1400 kJ/mol for the 3-nitrobenzyl anion radical) in comparison to other basic sites in the model peptide dication, facilitating exothermic proton transfer from one of the two sites of protonation. This interrupts the normal sequence of events in ECD or ETD leading to backbone fragmentation by forming a stable radical intermediate. The implications which these results have for previously proposed ECD and ETD mechanisms are discussed. PMID:19331417

Sohn, Chang Ho; Chung, Cheol K.; Yin, Sheng; Ramachandran, Prasanna; Loo, Joseph A.; Beauchamp, J. L.

2009-01-01

39

Evaluation of Affinity-Tagged Protein Expression Strategies using Local and Global Isotope Ratio Measurements  

SciTech Connect

Protein enrichments of engineered, affinity-tagged (or bait ) fusion proteins with interaction partners are often laden with background, non-specific proteins, due to interactions that occur in vitro as an artifact of the technique. Furthermore, the in vivo expression of the bait protein may itself affect physiology or metabolism. In this study, intrinsic affinity purification challenges were investigated in a model protein complex, DNA-dependent RNA polymerase (RNAP), encompassing chromosome- and plasmid-encoding strategies for bait proteins in two different microbial species: Escherichia coli and Rhodopseudomonas palustris. Isotope ratio measurements of bait protein expression strains relative to native, wild-type strains were performed by liquid chromatography tandem mass spectrometry (LC-MS-MS) to assess bait protein expression strategies in each species. Authentic interacting proteins of RNAP were successfully discerned from artifactual co-isolating proteins by the isotopic differentiation of interactions as random or targeted (I-DIRT) method (A. J. Tackett et al. J. Proteome Res. 2005, 4 (5), 1752-1756). To investigate broader effects of bait protein production in the bacteria, we compared proteomes from strains harboring a plasmid that encodes an affinity-tagged subunit (RpoA) of the RNAP complex with the corresponding wild-type strains using stable isotope metabolic labeling. The ratio of RpoA abundance in plasmid strains versus wild type was 0.8 for R. palustris and 1.7 for E. coli. While most other proteins showed no appreciable difference, proteins significantly increased in abundance in plasmid-encoded bait-expressing strains of both species included the plasmid encoded antibiotic resistance protein, GenR and proteins involved in amino acid biosynthesis. Together, these local, complex-specific and more global, whole proteome isotopic abundance ratio measurements provided a tool for evaluating both in vivo and in vitro effects of plasmid-encoding strategies for bait protein expression. This approach has the potential for enabling discovery of protein-protein interactions among the growing number of sequenced microbial species without the need for development of chromosomal insertion systems.

Hervey, IV, William Judson [ORNL; Khalsa-Moyers, Gurusahai K [ORNL; Lankford, Patricia K [ORNL; Owens, Elizabeth T [ORNL; McKeown, Catherine K [ORNL; Lu, Tse-Yuan S [ORNL; Foote, Linda J [ORNL; Morrell-Falvey, Jennifer L [ORNL; McDonald, W Hayes [ORNL; Pelletier, Dale A [ORNL; Hurst, Gregory {Greg} B [ORNL

2009-01-01

40

Vectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium  

PubMed Central

We constructed a series of expression vectors for purification of native proteins and protein complexes in Dictyostelium. Protein purification is achieved by either a C-terminal or N-terminal fusion of the protein of choice to the tandem affinity purification (TAP) tag. The TAP tag consists of a protein A tag and a calmodulin binding peptide (CBP) and has been successfully used for purification of native protein complexes from yeast and animal cells. Protein expression is driven by the constitutive actin 15 promoter and the vectors optionally carry additional green- or yellow fluorescent protein (GFP or YFP) tags for fusion at either a C- or N-terminal location. Tandem affinity purification of native Dictyostelium protein complexes was tested by using pArc-34, one of the members of the well characterized Dictyostelium Arp2/3 complex, as bait. After denaturation and SDS–PAGE separation of the pArc-34 associated proteins all members of the Arp2/3 complex could be identified. PMID:17296313

Meima, Marcel E.; Weening, Karin E.; Schaap, Pauline

2007-01-01

41

Application of a New Dual Localization-Affinity Purification Tag Reveals Novel Aspects of Protein Kinase Biology in Aspergillus nidulans  

PubMed Central

Filamentous fungi occupy critical environmental niches and have numerous beneficial industrial applications but devastating effects as pathogens and agents of food spoilage. As regulators of essentially all biological processes protein kinases have been intensively studied but how they regulate the often unique biology of filamentous fungi is not completely understood. Significant understanding of filamentous fungal biology has come from the study of the model organism Aspergillus nidulans using a combination of molecular genetics, biochemistry, cell biology and genomic approaches. Here we describe dual localization-affinity purification (DLAP) tags enabling endogenous N or C-terminal protein tagging for localization and biochemical studies in A. nidulans. To establish DLAP tag utility we endogenously tagged 17 protein kinases for analysis by live cell imaging and affinity purification. Proteomic analysis of purifications by mass spectrometry confirmed association of the CotA and NimXCdk1 kinases with known binding partners and verified a predicted interaction of the SldABub1/R1 spindle assembly checkpoint kinase with SldBBub3. We demonstrate that the single TOR kinase of A. nidulans locates to vacuoles and vesicles, suggesting that the function of endomembranes as major TOR cellular hubs is conserved in filamentous fungi. Comparative analysis revealed 7 kinases with mitotic specific locations including An-Cdc7 which unexpectedly located to mitotic spindle pole bodies (SPBs), the first such localization described for this family of DNA replication kinases. We show that the SepH septation kinase locates to SPBs specifically in the basal region of apical cells in a biphasic manner during mitosis and again during septation. This results in gradients of SepH between G1 SPBs which shift along hyphae as each septum forms. We propose that SepH regulates the septation initiation network (SIN) specifically at SPBs in the basal region of G1 cells and that localized gradients of SIN activity promote asymmetric septation. PMID:24599037

De Souza, Colin P.; Hashmi, Shahr B.; Osmani, Aysha H.; Osmani, Stephen A.

2014-01-01

42

Purification of recombinant histidine-tag streptolysin O using immobilized metal affinity expanded bed adsorption (IMA-EBA).  

PubMed

In this report, we describe the recombinant SLO expression as a fusion protein with a C-terminal hexahistidine tag and its purification using immobilized metal affinity expanded bed adsorption (STREAMLINE(trade mark) Chelating). In order to facilitate downstream processing of the purification, an efficient fermentation process was developed focusing on the achievement of high yields of soluble protein. The purification strategy resulted in a 40% recovery of active recombinant SLO and the protein was purified eight-fold. SDS-PAGE and Western-blot analysis of the purified protein revealed the presence of a 75 Mr form, which was the estimated relative Mass of the recombinant SLO. PMID:16529807

Camprubí, Sandra; Bruguera, Marc; Canalias, Francesca

2006-03-30

43

The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal  

E-print Network

The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity online 24 April 2011 Keywords: Proteases Detergent stability Membrane proteins a b s t r a c for integral membrane proteins contain detergents, which are required to maintain protein solubility. We

Lebendiker, Mario

44

Affinity-based SDS PAGE identification of phosphorylated Arabidopsis MAPKs and substrates by acrylamide pendant Phos-Tag™.  

PubMed

Protein phosphorylation is the most abundant and best studied protein posttranslational modification, dedicated to the regulation of protein function and subcellular localization as well as to protein-protein interactions. Identification and quantitation of the dynamic, conditional protein phosphorylation can be achieved by either metabolic labeling of the protein of interest with (32)P-labeled ATP followed by autoradiographic analysis, the use of specific monoclonal or polyclonal antibodies against the phosphorylated protein species and finally by phosphoproteome delineation using mass spectrometry.Hereby we present a fourth alternative which relies on the enforced-affinity-based-electrophoretic separation of phosphorylated from non-phosphorylated protein species by standard SDS-PAGE systems co-polymerized with Phos-Tag™ and Mn(2+) or Zn(2+) cations. Phosphate groups of phosphorylated Ser, Thr, and Tyr residues form complexes with Mn(2+) and Zn(2+) cations with polyacrylamide immobilized Phos-Tag™. Following appropriate treatment of the gels, separated proteins can be quantitatively transferred to PVDF or nitrocellulose membranes and probed with common-not phosphorylation state specific-antibodies and delineate the occurrence of a certain phosphoprotein species against its non-phosphorylated counterpart. PMID:24908119

Komis, George; Taká?, Tomáš; Bekešová, Slávka; Vadovi?, Pavol; Samaj, Jozef

2014-01-01

45

Selective Metabolite and Peptide Capture/Mass Detection using Fluorous Affinity Tags  

PubMed Central

A new and general methodology is described for the targeted enrichment and subsequent direct mass spectrometric characterization of sample subsets bearing various chemical functionalities from highly complex mixtures of biological origin. Specifically, sample components containing a chemical moiety of interest are first selectively labeled with perfluoroalkyl groups, and the entire sample is then applied to a perfluoroalkyl-silylated porous silicon (pSi) surface. Due to the unique hydrophobic and lipophobic nature of the perfluorinated tags, unlabeled sample components are readily removed using simple surface washes, and the enriched sample fraction can then directly be analyzed by desorption/ionization on silicon mass spectrometry (DIOS-MS). Importantly, this fluorous-based enrichment methodology provides a single platform that is equally applicable to both peptide as well as small molecule focused applications. The utility of this technique is demonstrated by the enrichment and mass spectrometric analysis of both various peptide subsets from protein digests as well as amino acids from serum. PMID:17343404

Go, Eden P.; Uritboonthai, Wilasinee; Apon, Junefredo V.; Trauger, Sunia A.; Nordstrom, Anders; O'Maille, Grace; Brittain, Scott M.; Peters, Eric C.; Siuzdak, Gary

2008-01-01

46

Novel Peptide Sequence (``IQ-tag'') with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging  

Microsoft Academic Search

BackgroundProbes that allow site-specific protein labeling have become critical tools for visualizing biological processes.MethodsHere we used phage display to identify a novel peptide sequence with nanomolar affinity for near infrared (NIR) (benz)indolium fluorochromes. The developed peptide sequence (“IQ-tag”) allows detection of NIR dyes in a wide range of assays including ELISA, flow cytometry, high throughput screens, microscopy, and optical in

Kimberly A. Kelly; Jonathan Carson; Jason R. McCarthy; Ralph Weissleder; Mark Isalan

2007-01-01

47

Comparative proteomics of glycoproteins based on lectin selection and isotope coding.  

PubMed

Lectins have been widely used in glycan structure analysis. The studies described here exploit this fact to select glycopeptides carrying disease-associated modifications in their oligosaccharides. Coupling lectin affinity selection with recent advances in stable isotope coding for quantitative proteomics allowed a comparative proteomics method to be developed for examining aberrant glycosylation in cancer. Control and experimental samples were individually tryptic digested and differentially coded with stable isotope coding agents before they were mixed and affinity selected with a lectin affinity chromatography column. Glycopeptides carrying an alpha-L-fucose residue were selected with Lotus tetragonolobus agglutinin (LTA) immobilized on a chromatography matrix. Because the oligosaccharides of glycoproteins are generally heterogeneous and often of unknown structure, it was necessary to deglycosylate the selected peptides with PNGase F before they could be compared to sequences in DNA and protein databases. After deglycosylated peptides were transferred to a reversed phase chromatography (RPC) column and fractionated by gradient elution with increasing amounts of acetonitrile. The RPC fractions were then analyzed by both matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) and electrospray ionization mass spectrometry (ESI-MS). When this method was applied to a study of lymphosarcoma in canines, it was found that during chemotherapy, a series of fucosylated proteins in the blood of patients decreased in concentration more than 2-fold. Two of the proteins identified, CD44 and E-selectin, are known to be involved in cell adhesion and cancer cell migration. The observed aberrant fucosylation of these proteins is consistent with the hypothesis that CD44 and E-selectin play a key role in metastasis and the spread of cancer cells to remote sites. PMID:14692455

Xiong, Li; Andrews, Dina; Regnier, Fred

2003-01-01

48

Studies with an immobilized metal affinity chromatography cassette system involving binuclear triazacyclononane-derived ligands: automation of batch adsorption measurements with tagged recombinant proteins.  

PubMed

This study describes the determination of the adsorption isotherms and binding kinetics of tagged recombinant proteins using a recently developed IMAC cassette system and employing automated robotic liquid handling procedures for IMAC resin screening. These results confirm that these new IMAC resins, generated from a variety of different metal-charged binuclear 1,4,7-triaza-cyclononane (tacn) ligands, interact with recombinant proteins containing a novel N-terminal metal binding tag, NT1A, with static binding capacities similar to those obtained with conventional hexa-His tagged proteins, but with significantly increased association constants. In addition, higher kinetic binding rates were observed with these new IMAC systems, an attribute that can be positively exploited to increase process productivity. The results from this investigation demonstrate that enhancements in binding capacities and affinities were achieved with these new IMAC resins and chosen NT1A tagged protein. Further, differences in the binding performances of the bis(tacn) xylenyl-bridged ligands were consistent with the distance between the metal binding centres of the two tacn moieties, the flexibility of the ligand and the potential contribution from the aromatic ring of the xylenyl group to undergo ?/? stacking interactions with the tagged proteins. PMID:24891160

Petzold, Martin; Coghlan, Campbell J; Hearn, Milton T W

2014-07-18

49

Purification of His-tagged hepatitis B core antigen from unclarified bacterial homogenate using immobilized metal affinity-expanded bed adsorption chromatography.  

PubMed

Hepatitis B core antigen (HBcAg) is used as a diagnostic reagent for the detection of hepatitis B virus infection. In this study, immobilized metal affinity-expanded bed adsorption chromatography (IMA-EBAC) was employed to purify N-terminally His-tagged HBcAg from unclarified bacterial homogenate. Streamline Chelating was used as the adsorbent and the batch adsorption experiment showed that the optimal binding pH of His-tagged HBcAg was 8.0 with a binding capacity of 1.8 mg per ml of adsorbent. The optimal elution condition for the elution of His-tagged HBcAg from the adsorbent was at pH 7 in the presence of 500 mM imidazole and 1.5 M NaCl. The IMA-EBAC has successfully recovered 56% of His-tagged HBcAg from the unclarified E. coli homogenate with a purification factor of 3.64. Enzyme-linked immunosorbent assay (ELISA) showed that the antigenicity of the recovered His-tagged HBcAg was not affected throughout the IMA-EBAC purification process and electron microscopy revealed that the protein assembled into virus-like particles (VLP). PMID:20388569

Yap, Wei Boon; Tey, Beng Ti; Alitheen, Noorjahan Banu Mohamed; Tan, Wen Siang

2010-05-21

50

A novel strategy for quantitative proteomics using isotope-coded protein labels.  

PubMed

Stable isotope labelling in combination with mass spectrometry has emerged as a powerful tool to identify and relatively quantify thousands of proteins within complex protein mixtures. Here we describe a novel method, termed isotope-coded protein label (ICPL), which is capable of high-throughput quantitative proteome profiling on a global scale. Since ICPL is based on stable isotope tagging at the frequent free amino groups of isolated intact proteins, it is applicable to any protein sample, including extracts from tissues or body fluids, and compatible to all separation methods currently employed in proteome studies. The method showed highly accurate and reproducible quantification of proteins and yielded high sequence coverage, indispensable for the detection of post-translational modifications and protein isoforms. The efficiency (e.g. accuracy, dynamic range, sensitivity, speed) of the approach is demonstrated by comparative analysis of two differentially spiked proteomes. PMID:15602776

Schmidt, Alexander; Kellermann, Josef; Lottspeich, Friedrich

2005-01-01

51

The AviD-tag, a NeutrAvidin/avidin specific peptide affinity tag for the immobilization and purification of recombinant proteins  

E-print Network

applications as well as for drug-delivery. Recently, we discovered a phage-display selected cyclic peptide believe the AviD-tag and its unique recognition properties will provide researchers with a useful new- spread use throughout biotechnology and are integral com- ponents of numerous research endeavors

Ghosh, Indraneel

52

Identifying Novel Protein Complexes in Cancer Cells Using Epitope-Tagging of Endogenous Human Genes and Affinity-Purification Mass Spectrometry  

PubMed Central

Affinity-purification mass spectrometry (AP-MS) is the preeminent technique for identification of eukaryotic protein complexes in vivo. AP-MS workflows typically express epitope-tagged bait proteins, immunopurify, and then identify associated protein complexes using mass spectrometry. However, challenges of existing strategies include the construction of expression vectors for large open reading frames and the possibility that overexpression of bait proteins may result in expression of nonphysiological levels of the bait protein with concomitant perturbation of endogenous protein complexes. To address these issues, we use human cell lines with epitope-tagged endogenous genes as AP-MS substrates to develop a platform that we call “knock-in AP-MS”, thereby avoiding the challenges of expression vector construction and ensuring that expression of tagged proteins is driven by endogenous regulatory mechanisms. Using three different bait genes (MRE11A, DNMT1 and APC), we show that cell lines expressing epitope-tagged endogenous genes make good substrates for sensitive and reproducible identification of protein interactions using AP-MS. In particular, we identify novel interactors of the important oncoprotein Adenomatous Polyposis Coli (APC), including an interaction with Flightless-1 homologue (FLII) that is enriched in nuclear fractions. PMID:23106643

Song, Jing; Hao, Yujun; Du, Zhanwen; Wang, Zhenghe; Ewing, Rob M.

2013-01-01

53

An isotope-coded fluorogenic cross-linker for high-performance target identification based on photoaffinity labeling.  

PubMed

A photoaffinity labeling (PAL)-based method for the rapid identification of target proteins is presented in which a high-performance chemical tag, an isotope-coded fluorescent tag (IsoFT), can be attached to the interacting site by irradiation. Labeled peptides can be easily distinguished among numerous proteolytic digests by sequential detection with highly sensitive fluorescence spectroscopy and mass spectrometry. Subsequent MS/MS analysis provides amino acid sequence information with a higher depth of coverage. The combination of PAL and heterogeneous target-selecting techniques significantly reduces the amount of time and protein required for identification. An additional photocleavable moiety successfully accelerated proteomic analysis using cell lysate. This method is a widely applicable approach for the rapid and accurate identification of interacting proteins. PMID:25382598

Tomohiro, Takenori; Morimoto, Shota; Shima, Toshiya; Chiba, Junya; Hatanaka, Yasumaru

2014-12-01

54

Purification of the alkaliphilic xylanases from Myceliophthora sp. IMI 387099 using cellulose-binding domain as an affinity tag  

Microsoft Academic Search

Ten xylanase isoforms produced by Myceliophthora sp. were characterized for their ability to bind to avicel. Three of the xylanases showing differential affinity for avicel\\u000a were purified by column chromatography. The purified xylanase Xyl IIa, IIb and IIc showed molecular mass of 47, 41 and 30 kDa\\u000a and pI of ?3.5, 4.8 and 5.2, respectively. Xyl IIa was optimally active at

A. K. Badhan; B. S. Chadha; H. S. Saini

2008-01-01

55

A novel two-zone protein uptake model for affinity chromatography and its application to the description of elution band profiles of proteins fused to a family 9 cellulose binding module affinity tag.  

PubMed

A novel two-zone model (TZM) is presented to describe the rate of solute uptake by the stationary phase of a sorption-type chromatography column. The TZM divides the porous stationary-phase particle into an inner protein-free core and an outer protein-containing zone where intraparticle transport is limited by pore diffusion and binding follows Langmuir theory. The TZM and the classic pore-diffusion model (PDM) of chromatography are applied to the prediction of stationary-phase uptake and elution bands within a cellulose-based affinity chromatography column designed to selectively purify proteins genetically labelled with a CBM9 (family 9 cellulose binding module) affinity tag. Under both linear and nonlinear loading conditions, the TZM closely matches rates of protein uptake within the stationary phase particles as measured by confocal laser scanning microscopy, while the PDM deviates from experiment in the linear-binding region. As a result, the TZM is shown to provide improved predictions of product breakthrough, including elution behavior from a bacterial lysate feed. PMID:17602694

Kavoosi, Mojgan; Sanaie, Nooshafarin; Dismer, Florian; Hubbuch, Jürgen; Kilburn, Douglas G; Haynes, Charles A

2007-08-10

56

LYTAG Two Phase is a protein purification system based on the use of two aqueous components. The method relies on the affinity of the protein tag LYTAG for one of the two  

E-print Network

, addition of choline led to migration of the - E. ColiCASCADE GFP LYTAG fusion protein, from the upperLYTAG Two Phase is a protein purification system based on the use of two aqueous components. The method relies on the affinity of the protein tag LYTAG for one of the two phase components, allowing

Lebendiker, Mario

57

Specific and reversible immobilization of proteins tagged to the affinity polypeptide C-LytA on functionalized graphite electrodes.  

PubMed

We have developed a general method for the specific and reversible immobilization of proteins fused to the choline-binding module C-LytA on functionalized graphite electrodes. Graphite electrode surfaces were modified by diazonium chemistry to introduce carboxylic groups that were subsequently used to anchor mixed self-assembled monolayers consisting of N,N-diethylethylenediamine groups, acting as choline analogs, and ethanolamine groups as spacers. The ability of the prepared electrodes to specifically bind C-LytA-tagged recombinant proteins was tested with a C-LytA-?-galactosidase fusion protein. The binding, activity and stability of the immobilized protein was evaluated by electrochemically monitoring the formation of an electroactive product in the enzymatic hydrolysis of the synthetic substrate 4-aminophenyl ?-D-galactopyranoside. The hybrid protein was immobilized in an specific and reversible way, while retaining the catalytic activity. Moreover, these functionalized electrodes were shown to be highly stable and reusable. The method developed here can be envisaged as a general, immobilization procedure on the protein biosensor field. PMID:24498237

Bello-Gil, Daniel; Maestro, Beatriz; Fonseca, Jennifer; Feliu, Juan M; Climent, Víctor; Sanz, Jesús M

2014-01-01

58

Specific and Reversible Immobilization of Proteins Tagged to the Affinity Polypeptide C-LytA on Functionalized Graphite Electrodes  

PubMed Central

We have developed a general method for the specific and reversible immobilization of proteins fused to the choline-binding module C-LytA on functionalized graphite electrodes. Graphite electrode surfaces were modified by diazonium chemistry to introduce carboxylic groups that were subsequently used to anchor mixed self-assembled monolayers consisting of N,N-diethylethylenediamine groups, acting as choline analogs, and ethanolamine groups as spacers. The ability of the prepared electrodes to specifically bind C-LytA-tagged recombinant proteins was tested with a C-LytA-?-galactosidase fusion protein. The binding, activity and stability of the immobilized protein was evaluated by electrochemically monitoring the formation of an electroactive product in the enzymatic hydrolysis of the synthetic substrate 4-aminophenyl ?-D-galactopyranoside. The hybrid protein was immobilized in an specific and reversible way, while retaining the catalytic activity. Moreover, these functionalized electrodes were shown to be highly stable and reusable. The method developed here can be envisaged as a general, immobilization procedure on the protein biosensor field. PMID:24498237

Bello-Gil, Daniel; Maestro, Beatriz; Fonseca, Jennifer; Feliu, Juan M.; Climent, Víctor; Sanz, Jesús M.

2014-01-01

59

A Method to Site-Specifically Identify and Quantitate Carbonyl End Products of Protein Oxidation Using Oxidation-Dependent Element Coded Affinity Tags (O-ECAT) and NanoLiquid Chromatography Fourier Transform Mass Spectrometry  

SciTech Connect

Protein oxidation is linked to cellular stress, aging, and disease. Protein oxidations that result in reactive species are of particular interest, since these reactive oxidation products may react with other proteins or biomolecules in an unmediated and irreversible fashion, providing a potential marker for a variety of disease mechanisms. We have developed a novel system to identify and quantitate, relative to other states, the sites of oxidation on a given protein. A specially designed Oxidation-dependent carbonyl-specific Element-Coded Affinity Mass Tag (O-ECAT), AOD, ((S)-2-(4-(2-aminooxy)-acetamido)-benzyl)-1, 4, 7, 10-tetraazacyclododecane-N, N', N'', N'''-tetraacetic acid, is used to covalently tag the residues of a protein oxidized to aldehyde or keto end products. After proteolysis, the resulting AOD-tagged peptides are affinity purified, and analyzed by nanoLC-FTICR-MS, which provides high specificity in extracting co-eluting AOD mass pairs with a unique mass difference and affords relative quantitation based on isotopic ratios. Using this methodology, we have mapped the surface oxidation sites on a model protein, recombinant human serum albumin (rHSA) in its native form (as purchased) and after FeEDTA oxidation. A variety of modified amino acid residues including lysine, arginine, proline, histidine, threonine, aspartic and glutamic acids, were found to be oxidized to aldehyde and keto end products. The sensitivity of this methodology is shown by the number of peptides identified, twenty peptides on the native protein and twenty-nine after surface oxidation using FeEDTA and ascorbate. All identified peptides map to the surface of the HSA crystal structure validating this method for identifying oxidized amino acids on protein surfaces. In relative quantitation experiments between FeEDTA oxidation and native protein oxidation, identified sites showed different relative propensities towards oxidation independent of amino acid residue. We expect to extend this methodology to study disease-related oxidation systems.

Lee, S; Young, N L; Whetstone, P A; Cheal, S M; Benner, W H; Lebrilla, C B; Meares, C F

2005-08-25

60

NLS-tagging: an alternative strategy to tag nuclear proteins  

PubMed Central

The characterization of transcription factor complexes and their binding sites in the genome by affinity purification has yielded tremendous new insights into how genes are regulated. The affinity purification requires either the use of antibodies raised against the factor of interest itself or by high-affinity binding of a C- or N-terminally added tag sequence to the factor. Unfortunately, fusing extra amino acids to the termini of a factor can interfere with its biological function or the tag may be inaccessible inside the protein. Here, we describe an effective solution to that problem by integrating the ‘tag’ close to the nuclear localization sequence domain of the factor. We demonstrate the effectiveness of this approach with the transcription factors Fli-1 and Irf2bp2, which cannot be tagged at their extremities without loss of function. This resulted in the identification of novel proteins partners and a new hypothesis on the contribution of Fli-1 to hematopoiesis. PMID:25260593

Giraud, Guillaume; Stadhouders, Ralph; Conidi, Andrea; Dekkers, Dick H.W.; Huylebroeck, Danny; Demmers, Jeroen A.A.; Soler, Eric; Grosveld, Frank G.

2014-01-01

61

Simplified Protein Purification Using an Autoprocessing, Inducible Enzyme Tag  

PubMed Central

Summary The development of affinity tags has greatly simplified protein purification procedures. A variety of affinity tags are now available to improve expression, solubility, and/or tag removal. In this chapter, we describe a method for purifying recombinant proteins expressed in Escherichia coli that uses a highly specific, inducible, C-terminal autoprocessing protease tag. This method streamlines affinity purification, cleavage, and tag separation into a one-step purification procedure, avoiding the need to remove fusion tags from target proteins with exogenous proteases. In addition to accelerating protein purification, we show that this method can enhance the expression, stability, and solubility of select proteins. PMID:24943314

Shen, Aimee

2015-01-01

62

Semi-quantitative Measurement of a Specific Glycoform Using a DNA-tagged Antibody and Lectin Affinity Chromatography for Glyco-biomarker Development.  

PubMed

Aberrant glycosylation-targeted disease biomarker development is based on cumulative evidence that certain glycoforms are mass-produced in a disease-specific manner. However, the development process has been hampered by the absence of an efficient validation method based on a sensitive and multiplexed platform. In particular, ELISA-based analytical tools are not adequate for this purpose, mainly because of the presence of a pair of N-glycans of IgG-type antibodies. To overcome the associated hurdles in this study, antibodies were tagged with oligonucleotides with T7 promoter and then allowed to form a complex with corresponding antigens. An antibody-bound specific glycoform was isolated by lectin chromatography and quantitatively measured on a DNA microarray chip following production of fluorescent RNA by T7-trascription. This tool ensured measurement of targeted glycoforms of multiple biomarkers with high sensitivity and multiplexity. This analytical method was applied to an in vitro diagnostic multivariate index assay where a panel of hepatocellular carcinoma (HCC) biomarkers comprising alpha-fetoprotein, hemopexin, and alpha-2-macroglobulin (A2M) was examined in terms of the serum level and their fuco-fractions. The results indicated that the tests using the multiplexed fuco-biomarkers provided improved discriminatory power between non- hepatocellular carcinoma and hepatocellular carcinoma subjects compared with the alpha-fetoprotein level or fuco-alpha-fetoprotein test alone. The developed method is expected to facilitate the validation of disease-specific glycan biomarker candidates. PMID:25525205

Lee, Ju Hee; Cho, Chang Hee; Kim, Sun Hee; Kang, Jeong Gu; Yoo, Jong Shin; Chang, Chulhun Ludgerus; Ko, Jeong-Heon; Kim, Yong-Sam

2015-03-01

63

A Phosphorylation Tag for Uranyl Mediated Protein Purification and Photo Assisted Tag Removal  

PubMed Central

Most protein purification procedures include an affinity tag fused to either the N or C-terminal end of the protein of interest as well as a procedure for tag removal. Tag removal is not straightforward and especially tag removal from the C-terminal end is a challenge due to the characteristics of enzymes available for this purpose. In the present study, we demonstrate the utility of the divalent uranyl ion in a new procedure for protein purification and tag removal. By employment of a GFP (green florescence protein) recombinant protein we show that uranyl binding to a phosphorylated C-terminal tag enables target protein purification from an E. coli extract by immobilized uranyl affinity chromatography. Subsequently, the tag can be efficiently removed by UV-irradiation assisted uranyl photocleavage. We therefore suggest that the divalent uranyl ion (UO22+) may provide a dual function in protein purification and subsequent C-terminal tag removal procedures. PMID:24599526

Zhang, Qiang; Jørgensen, Thomas J. D.; Nielsen, Peter E.; Møllegaard, Niels Erik

2014-01-01

64

Tagging Walruses  

USGS Multimedia Gallery

A transmitter tag (left) is being deployed by a USGS Wildlife Biologist (far right). Transmitter tags are deployed on the back of walruses where their skin is thickest and where their data transmissions may be received from passing satellites. Tag deployment happens in the blink of an eye with the ...

65

BiPS, a photocleavable, isotopically coded, fluorescent cross-linker for structural proteomics.  

PubMed

Cross-linking combined with mass spectrometry is an emerging approach for studying protein structure and protein-protein interactions. However, unambiguous mass spectrometric identification of cross-linked peptides derived from proteolytically digested cross-linked proteins is still challenging. Here we describe the use of a novel cross-linker, bimane bisthiopropionic acid N-succinimidyl ester (BiPS), that overcomes many of the challenges associated with other cross-linking reagents. BiPS is distinguished from other cross-linkers by a unique combination of properties: it is photocleavable, fluorescent, homobifunctional, amine-reactive, and isotopically coded. As demonstrated with a model protein complex, RNase S, the fluorescent moiety of BiPS allows for sensitive and specific monitoring of the different cross-linking steps, including detection and isolation of cross-linked proteins by gel electrophoresis, determination of in-gel digestion completion, and fluorescence-based separation of cross-linked peptides by HPLC. The isotopic coding of BiPS results in characteristic ion signal "doublets" in mass spectra, thereby permitting ready detection of cross-linker-containing peptides. Under MALDI-MS conditions, partial photocleavage of the cross-linker occurs, releasing the cross-linked peptides. This allows differentiation between dead-end, intra-, and interpeptide cross-links based on losses of specific mass fragments. It also allows the use of the isotope doublets as mass spectrometric "signatures." A software program was developed that permits automatic cross-link identification and assignment of the cross-link type. Furthermore photocleavage of BiPS assists in cross-link identification by allowing separate tandem mass spectrometry sequencing of each peptide comprising the original cross-link. By combining the use of BiPS with MS, we have provided the first direct evidence for the docking site of a phosphorylated G-protein-coupled receptor C terminus on the multifunctional adaptor protein beta-arrestin, clearly demonstrating the broad potential and application of this novel cross-linker in structural and cellular biology. PMID:18838738

Petrotchenko, Evgeniy V; Xiao, Kunhong; Cable, Jennifer; Chen, Yiwen; Dokholyan, Nikolay V; Borchers, Christoph H

2009-02-01

66

Epitope Discovery for a Synthetic Polymer Nanoparticle: A New Strategy for Developing a Peptide Tag  

PubMed Central

We describe a novel epitope discovery strategy for creating an affinity agent/peptide tag pair. A synthetic polymer nanoparticle (NP) was used as the “bait” to catch an affinity peptide tag. Biotinylated peptide tag candidates of varied sequence and length were attached to an avidin platform and screened for affinity against the polymer NP. NP affinity for the avidin/peptide tag complexes was used to provide insight into factors that contribute NP/tag binding. The identified epitope sequence with an optimized length (tMel-tag) was fused to two recombinant proteins. The tagged proteins exhibited higher NP affinity than proteins without tags. The results establish that a fusion peptide tag consisting of optimized 15 amino acid residues can provide strong affinity to an abiotic polymer NP. The affinity and selectivity of NP/tMel-tag interactions were exploited for protein purification in conjunction with immobilized metal ion/His6-tag interactions to prepare highly purified recombinant proteins. This strategy makes available inexpensive, abiotic synthetic polymers as affinity agents for peptide tags and provides alternatives for important applications where more costly affinity agents are used. PMID:24410250

2015-01-01

67

Isotope Coded Protein Labeling analysis of plasma specimens from acute severe dengue fever patients  

PubMed Central

Background Dengue fever is the most important arthropod born viral disease of public health significance. Although most patients suffer only from flu-like symptoms, a small group of patient experiences more severe forms of the disease. To contribute to a better understanding of its pathogenesis this study aims to identify proteins differentially expressed in a pool of five viremic plasma from severe dengue patients relative to a pool of five non-severe dengue patients. Results The use of Isotope Coded Protein Labeling (ICPLTM) to analyze plasma depleted of twenty high-abundance proteins allowed for the identification of 51 differentially expressed proteins, which were characterized by mass spectrometry. Using quantitative ELISA, three of these proteins (Leucine-rich glycoprotein 1, Vitamin D binding-protein and Ferritin) were confirmed as having an increased expression in a panel of severe dengue plasma. The proteins identified as overexpressed by ICPLTM in severe dengue plasma involve in clear up action after cell injury, tissue coherence and immune defense. Conclusion This ICPLTM study evaluating differences between acute severe dengue plasmas and acute non-severe dengue plasmas suggests that the three proteins identified are overexpressed early in the course of the disease. Their possible use as biomarkers for the prognostic of disease severity is discussed. PMID:23101585

2012-01-01

68

Glowing tags  

Microsoft Academic Search

Tagging physical objects to get a link from the physical world into some kind of technology has been done for a long time. The most commonly known is probably the barcodes that is used in five billion scans every day. During a project at Xerox Research Centre Europe in Cambridge, we came across the concept of Glow Tags. It is

Hans Tap

69

Stable isotope coded derivatizing reagents as internal standards in metabolite profiling.  

PubMed

Gas chromatography (GC) and liquid chromatography (LC) coupled to mass spectrometric (MS) detection have become the two main techniques for the analysis of metabolite pools (i.e. Metabolomics). These technologies are especially suited for Metabolite Profiling analysis of various metabolite groups due to high separation capabilities of the chromatographs and high sensitivity of the mass analysers. The trend in quantitative Metabolite Profiling is to add more metabolites and metabolite groups in a single method. This should not be done by compromising the analytical precision. Mass spectrometric detection comes with certain limitations, especially in the quantitative aspects as standards are needed for conversion of ion abundance to concentration and ionization efficiencies are directly dependent on eluent conditions. This calls for novel strategies to counteract all variables that can influence the quantitative precision. Usually, internal standards are used to correct any technical variation. For quantitation of single or just a few analytes this can be executed with spiking isotopically labeled standards. However, for more comprehensive analytical tasks, e.g. profiling tens or hundreds of analytes simultaneously, this strategy becomes expensive and in many cases isotopically labeled standards are not available. An alternative is to introduce a derivatizing step where the sample is derivatized with naturally labeled reagent, while a standard solution is separately derivatized with isotopically labeled reagent and spiked into the sample solution prior to analysis. This strategy, named isotope coded derivatization - ICD, is attractive in the emerging field of quantitative Metabolite Profiling where current protocols can easily comprise over hundred metabolites. This review provides an overview of isotopically labeled derivatizing reagents that have been developed for important metabolite groups with the aim to improve analytical performance and precision. PMID:23628173

Bruheim, Per; Kvitvang, Hans Fredrik Nyvold; Villas-Boas, Silas G

2013-06-28

70

Affinity Chromatography  

NSDL National Science Digital Library

This is an experiment showing the application of affinity chromatography to the separation of albumin from horse serum. A brief introduction of affinity chromatography and how it is being used in this specific experiment is given. This appears to be a good experiment to show the advantages of affinity chromatography in separating specific proteins from a complex matrix and would be useful in a biochemistry course or a course that is specifically looking at differing types of chromatography.

DiResta, Dan

71

Affinity Chromatography  

NSDL National Science Digital Library

Using exposition, graphics, and commercial videos, this module teaches the theory and application of affinity chromatography in the characterization of proteins, nucleic acids, and other biochemical/biomedical systems. Problems and application examples support the tutorial material.

72

Germ Tag  

NSDL National Science Digital Library

In this version of tag, a large group of learners model how the body fights infection. Learners act as germs, as lymphocytes, and as the body's cells threatened by germs. After playing one round, subsequent rounds can use different numbers of germs and/or lymphocytes to see how the infection rate is changed. When learners set up a free account at Kinetic City, they can answer bonus questions at the end of the activity as a quick assessment. They can also keep track of their progress in all of the Kinetic City activities, and compare their progress to other participants worldwide.

American Association for the Advancement of Science

2009-01-01

73

Deciphering systemic wound responses of the pumpkin extrafascicular phloem by metabolomics and stable isotope-coded protein labeling.  

PubMed

In cucurbits, phloem latex exudes from cut sieve tubes of the extrafascicular phloem (EFP), serving in defense against herbivores. We analyzed inducible defense mechanisms in the EFP of pumpkin (Cucurbita maxima) after leaf damage. As an early systemic response, wounding elicited transient accumulation of jasmonates and a decrease in exudation probably due to partial sieve tube occlusion by callose. The energy status of the EFP was enhanced as indicated by increased levels of ATP, phosphate, and intermediates of the citric acid cycle. Gas chromatography coupled to mass spectrometry also revealed that sucrose transport, gluconeogenesis/glycolysis, and amino acid metabolism were up-regulated after wounding. Combining ProteoMiner technology for the enrichment of low-abundance proteins with stable isotope-coded protein labeling, we identified 51 wound-regulated phloem proteins. Two Sucrose-Nonfermenting1-related protein kinases and a 32-kD 14-3-3 protein are candidate central regulators of stress metabolism in the EFP. Other proteins, such as the Silverleaf Whitefly-Induced Protein1, Mitogen Activated Protein Kinase6, and Heat Shock Protein81, have known defensive functions. Isotope-coded protein labeling and western-blot analyses indicated that Cyclophilin18 is a reliable marker for stress responses of the EFP. As a hint toward the induction of redox signaling, we have observed delayed oxidation-triggered polymerization of the major Phloem Protein1 (PP1) and PP2, which correlated with a decline in carbonylation of PP2. In sum, wounding triggered transient sieve tube occlusion, enhanced energy metabolism, and accumulation of defense-related proteins in the pumpkin EFP. The systemic wound response was mediated by jasmonate and redox signaling. PMID:23085839

Gaupels, Frank; Sarioglu, Hakan; Beckmann, Manfred; Hause, Bettina; Spannagl, Manuel; Draper, John; Lindermayr, Christian; Durner, Jörg

2012-12-01

74

Aptamer-modified magnetic beads in affinity separation of proteins.  

PubMed

Aptamers are valuable alternative ligands for affinity separations. Here, we describe the aptamer-based affinity separation of His-tagged proteins using an aptamer directed against the His-tag. The immobilization of the aptamer to magnetic beads is described as well as the aptamer-based purification and proper methods for the characterization of the process. Moreover, indications for the transfer of the process to other aptamers are given. PMID:25749947

Zhu, Guohong; Walter, Johanna-Gabriela

2015-01-01

75

Shark Tagging Activities.  

ERIC Educational Resources Information Center

In this group activity, children learn about the purpose of tagging and how scientists tag a shark. Using a cut-out of a shark, students identify, measure, record data, read coordinates, and tag a shark. Includes introductory information about the purpose of tagging and the procedure, a data sheet showing original tagging data from Tampa Bay, and…

Current: The Journal of Marine Education, 1998

1998-01-01

76

Visualizing tags over time  

Microsoft Academic Search

We consider the problem of visualizing the evolution of tags within the Flickr (flickr.com) online image sharing com- munity. Any user of the Flickr service may append a tag to any photo in the system. Over the past year, users have on average added over a million tags each week. Under- standing the evolution of these tags over time is

Micah Dubinko; Ravi Kumar; Joseph Magnani; Jasmine Novak; Prabhakar Raghavan; Andrew Tomkins

2006-01-01

77

Isotopically-coded short-range hetero-bifunctional photo-reactive crosslinkers for studying protein structure.  

PubMed

The resolution and the fidelity of a protein structural model, constructed using crosslinking data, is dependent on the crosslinking distance constraints. Most of the popular amine-reactive NHS-ester crosslinkers are limited in their capacity to provide short distance constraints because of the rarity of lysine residues occurring in close proximity in the protein structure. To solve this problem, hetero-bifunctional crosslinkers containing both a photo-reactive functional group and an NHS-ester group can be used to enable non-specific crosslinking within the proximity of these lysine residues. Here we develop three such isotopically-coded hetero-bifunctional photo-reactive crosslinkers, bearing azido, diazirine or benzophenone photo-reactive groups (azido-benzoic-acid-succinimide (ABAS)-(12)C6/(13)C6, succinimidyl-diazirine (SDA)-(12)C5/(13)C5, and carboxy-benzophenone-succinimide (CBS)-(12)C6/(13)C6, respectively). These crosslinkers were validated using several model proteins/peptides and were then applied to study the structure of the native ?-synuclein protein. In that case the ABAS crosslinker proved to be the most suitable, with 10 crosslinks being found in the native ?-synuclein structure. PMID:25192908

Brodie, Nicholas I; Makepeace, Karl A T; Petrotchenko, Evgeniy V; Borchers, Christoph H

2014-09-01

78

Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in  

E-print Network

). However, MBP fusion proteins do not always bind efficiently to amylose resin (Pryor and Leiting 1997; Routzahn and Waugh 2002); and even when they do, amylose affinity chromatography typi- cally does the limitations of amylose affinity chromatography, Routzahn and Waugh incor- porated auxiliary affinity tags

79

Hexahistidine-Tagged Maltose-Binding Protein as a Fusion Partner for the Production of Soluble  

E-print Network

solubility-enhancing protein that is also a natural affinity tag. In principle, its affinity for amylose, however, we and others have observed that amylose affinity chromatography has several noteworthy efficiently to amylose resin; and, even when they do, this technique rarely yields samples of sufficient

80

Protein structure modeling indicates hexahistidine-tag interference with enzyme activity.  

PubMed

Unusual kinetic characteristics of tropinone reductase, an enzyme in the family of short chain dehydrogenases, prompted to investigate a possible impact of the hexahistidine affinity tag on catalytic properties. Comparison of enzymes from Solanum dulcamara, Solanaceae, tagged at the N-terminus or at the C-terminus revealed that the C-terminally tagged form was functionally impaired. Protein modeling indicated that the hexahistidine tag attached at the C-terminus but not at the N-terminus of the polypeptide can interfere with the active site by steric or electrostatic interactions. In consequence, protein modeling is suggested before enzyme expression with affinity tags to estimate possible interactions of affinity tags with the active center. PMID:18214963

Freydank, Anna-Carolin; Brandt, Wolfgang; Dräger, Birgit

2008-07-01

81

DESIGN, SYNTHESIS, AND APPLICATION OF THE TRIMETHOPRIM-BASED CHEMICAL TAG FOR LIVE CELL IMAGING  

PubMed Central

Over the past decade chemical tags have been developed to complement the use of fluorescent proteins in live cell imaging. Chemical tags retain the specificity of protein labeling achieved with fluorescent proteins through genetic encoding, but provide smaller, more robust tags and modular use of organic fluorophores with high photon-output and tailored functionalities. The trimethoprim-based chemical tag (TMP-tag) was initially developed based on the high affinity interaction between E.coli dihydrofolatereductase and the antibiotic trimethoprim and subsequently rendered covalent and fluorogenic via proximity-induced protein labeling reactions. To date, the TMP-tag is one of the few chemical tags that enable intracellular protein labeling and high-resolution live cell imaging. Here we describe the general design, chemical synthesis, and application of TMP-tag for live cell imaging. Alternative protocols for synthesizing and using the covalent and the fluorogenic TMP-tags are also included. PMID:23839994

Jing, Chaoran; Cornish, Virginia W.

2013-01-01

82

Donor Tag Game  

MedlinePLUS

... Games > Donor Tag Game Printable Version Donor Tag Game This feature requires version 6 or later of ... Blood Donor Community Donor Stories Recipient Stories SleevesUp Games Facebook Fanbox Avatars and Badges Banners eCards Enter ...

83

Visualization of Tag Sequence  

Microsoft Academic Search

Tags, the user-generated metadata for web resources, have been widely used in social networking systems, such as del.icio.us, Flickr, Youtube and Facebook. Tagging technology is deserved to be studied for education because it can reveal the pattern of users¿ knowledge learning and sharing. In this paper, we analyze users' tagging activities by adopting some visualization methods upon tag sequence. The

Chao Wu; Hang Zhou

2008-01-01

84

TagAssist: Automatic Tag Suggestion for Blog Posts  

Microsoft Academic Search

In this paper, we describe a system called TagAssist that provides tag suggestions for new blog posts by utilizing existing tagged posts. The system is able to increase the quality of suggested tags by performing lossless compression over existing tag data. In addition, the system employs a set of metrics to evaluate the quality of a potential tag suggestion. Coupled

Sanjay C. Sood; Kristian J. Hammond; Sara H. Owsley; Larry Birnbaum

2007-01-01

85

Cutaneous skin tag  

MedlinePLUS

Skin tag; Acrochordon; Fibroepithelial polyp ... have diabetes. They are thought to occur from skin rubbing against skin. ... The tag sticks out of the skin and may have a short, narrow stalk connecting it to the surface of the skin. Some skin tags are as long as ...

86

RFID Tag Ownership Transfer  

Microsoft Academic Search

In some applications, the bearer of a radio frequency identi- fication (RFID) tag might change, with corresponding changes required in the RFID system infrastructure. We survey the security requirements for RFID tag ownership transfer, and propose novel authentication pro- tocols for tag ownership and authorisation transfer. The proposed proto- cols satisfy most of the requirements that we present, and have

Boyeon Song

87

Extracting Tag Hierarchies  

PubMed Central

Tagging items with descriptive annotations or keywords is a very natural way to compress and highlight information about the properties of the given entity. Over the years several methods have been proposed for extracting a hierarchy between the tags for systems with a "flat", egalitarian organization of the tags, which is very common when the tags correspond to free words given by numerous independent people. Here we present a complete framework for automated tag hierarchy extraction based on tag occurrence statistics. Along with proposing new algorithms, we are also introducing different quality measures enabling the detailed comparison of competing approaches from different aspects. Furthermore, we set up a synthetic, computer generated benchmark providing a versatile tool for testing, with a couple of tunable parameters capable of generating a wide range of test beds. Beside the computer generated input we also use real data in our studies, including a biological example with a pre-defined hierarchy between the tags. The encouraging similarity between the pre-defined and reconstructed hierarchy, as well as the seemingly meaningful hierarchies obtained for other real systems indicate that tag hierarchy extraction is a very promising direction for further research with a great potential for practical applications. Tags have become very prevalent nowadays in various online platforms ranging from blogs through scientific publications to protein databases. Furthermore, tagging systems dedicated for voluntary tagging of photos, films, books, etc. with free words are also becoming popular. The emerging large collections of tags associated with different objects are often referred to as folksonomies, highlighting their collaborative origin and the “flat” organization of the tags opposed to traditional hierarchical categorization. Adding a tag hierarchy corresponding to a given folksonomy can very effectively help narrowing or broadening the scope of search. Moreover, recommendation systems could also benefit from a tag hierarchy. PMID:24391901

Tibély, Gergely; Pollner, Péter; Vicsek, Tamás; Palla, Gergely

2013-01-01

88

Preparation and immunogenicity of tag-free recombinant human eppin  

PubMed Central

Human epididymal protease inhibitor (eppin) may be effective as a male contraceptive vaccine. In a number of studies, eppin with an engineered His6-tag has been produced using prokaryotic expression systems. For production of pharmaceutical-grade proteins for human use, however, the His6-tag must be removed. This study describes a method for producing recombinant human eppin without a His6-tag. We constructed plasmid pET28a (+)-His6-tobacco etch virus (TEV)-eppin for expression in Escherichia coli. After purification and refolding, the fusion protein His6-TEV-eppin was digested with TEV protease to remove the His6-tag and was further purified by NTA-Ni2+ affinity chromatography. Using this procedure, 2 mg of eppin without a His6-tag was isolated from 1 l of culture with a purity of >95%. The immunogenicity of the eppin was characterized using male Balb/c mice. PMID:21892195

Zhang, Jie; Ding, Xin-Liang; Bian, Zeng-Hui; Xia, Yan-Kai; Wang, Shou-Lin; Song, Ling; Wang, Xin-Ru

2011-01-01

89

Demand-Driven Tag Recommendation  

Microsoft Academic Search

\\u000a Collaborative tagging allows users to assign arbitrary keywords (or tags) describing the content of objects, which facilitates\\u000a navigation and improves searching without dependence on pre-configured categories. In large-scale tag-based systems, tag recommendation\\u000a services can assist a user in the assignment of tags to objects and help consolidate the vocabulary of tags across users.\\u000a A promising approach for tag recommendation is

Guilherme Vale Menezes; Jussara M. Almeida; Fabiano Belém; Marcos André Gonçalves; Anísio Lacerda; Edleno Silva de Moura; Gisele L. Pappa; Adriano Veloso; Nivio Ziviani

2010-01-01

90

A novel method of affinity-purifying proteins using a bis-arsenical fluorescein.  

PubMed Central

Genetically-encoded affinity tags constitute an important strategy for purifying proteins. Here, we have designed a novel affinity matrix based on the his-arsenical fluorescein dye FlAsH, which specifically recognizes short alpha-helical peptides containing the sequence CCXXCC (Griffin BA, Adams SR, Tsien RY, 1998, Science 281:269-272). We find that kinesin tagged with this cysteine-containing helix binds specifically to FlAsH resin and can be eluted in a fully active form. This affinity tag has several advantages over polyhistidine, the only small affinity tag in common use. The protein obtained with this single chromatographic step from crude Escherichia coli lysates is purer than that obtained with nickel affinity chromatography of 6xHis tagged kinesin. Moreover, unlike nickel affinity chromatography, which requires high concentrations of imidazole or pH changes for elution, protein bound to the FlAsH column can be completely eluted by dithiothreitol. Because of these mild elution conditions, FlAsH affinity chromatography is ideal for recovering fully active protein and for the purification of intact protein complexes. PMID:10716173

Thorn, K. S.; Naber, N.; Matuska, M.; Vale, R. D.; Cooke, R.

2000-01-01

91

New Tags for Recombinant Protein Detection and O-Glycosylation Reporters  

PubMed Central

Monoclonal antibodies (mAbs), because of their unique specificity, are irreplaceable tools for scientific research. Precise mapping of the antigenic determinants allows the development of epitope tagging approaches to be used with recombinant proteins for several purposes. Here we describe a new family of tags derived from the epitope recognized by a single highly specific mAb (anti-roTag mAb), which was obtained from a pool of mAbs reacting with the rotavirus nonstructural protein 5 (NSP5). The variable regions of the anti-roTag mAb were identified and their binding capacity verified upon expression as a single-chain/miniAb. The minimal epitope, termed roTag, was identified as a 10 amino acid sequence (SISSSIFKNE). The affinity of the anti-roTag/roTag interaction was found to be comparable to that of the anti-SV5/SV5 tag interaction. roTag was successfully used for detection of several recombinant cytosolic, secretory and membrane proteins. Two additional variants of roTag of 10 and 13 amino acids containing O-glycosylation susceptible sites (termed OG-tag and roTagO) were constructed and characterised. These tags were useful to detect proteins passing through the Golgi apparatus, the site of O-glycosylation. PMID:24802141

Arnoldi, Francesca; Burrone, Oscar R.

2014-01-01

92

Enhancing the Solubility of Recombinant Proteins in Escherichia coli by Using Hexahistidine-Tagged  

E-print Network

of or in addition to amylose affinity chromatography. This chapter describes a generic method for the over is the only solubility- enhancing protein that is also a natural affinity tag. Unfortunately, however, amylose binding capacity of amylose resin for MBP but also because of persis- tent contaminants that require

93

Purification and characterisation of recombinant His-tagged RgpB gingipain from Porphymonas gingivalis.  

PubMed

Gingipain proteases are important virulence factors from the periodontal pathogen Porphyromonas gingivalis and are the target of many in vitro studies. Due to their close biochemical properties, purification of individual gingipains is difficult and requires multiple chromatographic steps. In this study, we demonstrate that insertion of a hexahistidine affinity tag upstream of a C-terminal outer membrane translocation signal in RgpB gingipain leads to the secretion of a soluble, mature form of RgpB bearing the affinity tag that can easily be purified by nickel-chelating affinity chromatography. The final product obtained high yielding high purity is biochemically indistinguishable from the native RgpB enzyme. PMID:25720118

Veillard, Florian; Potempa, Barbara; Guo, Yonghua; Ksiazek, Miroslaw; Sztukowska, Maryta N; Houston, John A; Koneru, Lahari; Nguyen, Ky-Anh; Potempa, Jan

2015-04-01

94

Deciphering Systemic Wound Responses of the Pumpkin Extrafascicular Phloem by Metabolomics and Stable Isotope-Coded Protein Labeling1[C][W  

PubMed Central

In cucurbits, phloem latex exudes from cut sieve tubes of the extrafascicular phloem (EFP), serving in defense against herbivores. We analyzed inducible defense mechanisms in the EFP of pumpkin (Cucurbita maxima) after leaf damage. As an early systemic response, wounding elicited transient accumulation of jasmonates and a decrease in exudation probably due to partial sieve tube occlusion by callose. The energy status of the EFP was enhanced as indicated by increased levels of ATP, phosphate, and intermediates of the citric acid cycle. Gas chromatography coupled to mass spectrometry also revealed that sucrose transport, gluconeogenesis/glycolysis, and amino acid metabolism were up-regulated after wounding. Combining ProteoMiner technology for the enrichment of low-abundance proteins with stable isotope-coded protein labeling, we identified 51 wound-regulated phloem proteins. Two Sucrose-Nonfermenting1-related protein kinases and a 32-kD 14-3-3 protein are candidate central regulators of stress metabolism in the EFP. Other proteins, such as the Silverleaf Whitefly-Induced Protein1, Mitogen Activated Protein Kinase6, and Heat Shock Protein81, have known defensive functions. Isotope-coded protein labeling and western-blot analyses indicated that Cyclophilin18 is a reliable marker for stress responses of the EFP. As a hint toward the induction of redox signaling, we have observed delayed oxidation-triggered polymerization of the major Phloem Protein1 (PP1) and PP2, which correlated with a decline in carbonylation of PP2. In sum, wounding triggered transient sieve tube occlusion, enhanced energy metabolism, and accumulation of defense-related proteins in the pumpkin EFP. The systemic wound response was mediated by jasmonate and redox signaling. PMID:23085839

Gaupels, Frank; Sarioglu, Hakan; Beckmann, Manfred; Hause, Bettina; Spannagl, Manuel; Draper, John; Lindermayr, Christian; Durner, Jörg

2012-01-01

95

TAG Advertisement Hardware  

NASA Technical Reports Server (NTRS)

LaRc SI Material Overall photograph showing the material specimens, the graphite composite, the gold composite and the molded gears on a black background. These photos were used for the TAG CO-OP Public Relations and promotions

1994-01-01

96

Neuron Chain Tag  

NSDL National Science Digital Library

In this outdoor activity, learners play a game of Tag to discover how neurons attach themselves to each other to form a chain. The game starts with one learner who is "it" and represents the first neuron. When "it" tags another player, the tagger player must hold the hand of "it" and work together to form a long a chain. The game ends when all the players are part of the neuron chain.

2012-06-26

97

Facets: Ersatz, Resource and Tag  

ERIC Educational Resources Information Center

Introduction: Faceted classification appears to be of utmost importance. Ersatz facets, resource faceting and tag faceting: The distinctions are drawn between facets and ersatz facets, and between faceted resources and faceted tags. Single tag resource faceting and multiple tag information object faceting: The basic features are explored of single…

Frické, Martin H.

2013-01-01

98

Affinity Through Instant Messaging  

E-print Network

://kuscholarworks.ku.edu/dspace/ To appear as: Grebe, J. P., & Hall, J. A. (in press). Affinity in instant messaging. Northwest Journal of Communication. RUNNING HEAD: AFFINITY THROUGH IM Affinity Through Instant Messaging Jason P. Grebe, M.A. & Jeffrey A. Hall, Ph... is signaled, perceived, and detected during an initial interaction through instant messaging (IM) between two opposite-sex strangers. The purpose of this study is to determine the relationship between overall liking of the conversational partner and three...

Grebe, Jason P.; Hall, Jeffrey A.

2012-01-01

99

Affine Defects and Gravitation  

E-print Network

We argue that the structure general relativity (GR) as a theory of affine defects is deeper than the standard interpretation as a metric theory of gravitation. Einstein-Cartan theory (EC), with its inhomogenous affine symmetry, should be the standard-bearer for GR-like theories. A discrete affine interpretation of EC (and gauge theory) yields topological definitions of momentum and spin (and Yang Mills current), and their conservation laws become discrete topological identities. Considerations from quantum theory provide evidence that discrete affine defects are the physical foundation for gravitation.

R. J. Petti

2014-12-12

100

For polyhistidine-tagged protein purification BD Biosciences Clontech www.clontech.com 800-662-2566  

E-print Network

For polyhistidine-tagged protein purification BD Biosciences Clontech · www.clontech.com · 800;4 BD Biosciences Clontech · www.clontech.com · 800-662-2566 TALON Resins are durable, cobalt-based IMAC COO­ COO­ COO­ Co2+Carrier Bead TALONTM His tag Protein #12;TALONTM Metal Affinity Resin...cont. BD

Lebendiker, Mario

101

Quantitative evaluation of his-tag purification and immunoprecipitation of tristetraprolin and its mutant proteins from transfected human cells  

Technology Transfer Automated Retrieval System (TEKTRAN)

Histidine (His)-tag is widely used for affinity purification of recombinant proteins, but the yield and purity of expressed proteins are quite different. Little information is available about quantitative evaluation of this procedure. The objective of the current study was to evaluate the His-tag pr...

102

Discourse Tagging Tool and Discourse-Tagged Multilingual Corpora  

Microsoft Academic Search

As a part of our on-going research on multilingual anaphora resolution (cf. Aone and McKee [3],Aone [1]), we have built a graphical tool to tag texts with antecedent-anaphor relations and havecreated corpora tagged with such relations. The tool, called the Discourse Tagging Tool (DTTool),has been used to manually tag Japanese, Spanish and English texts with anaphora, their types (suchas pronouns

Chinatsu Aone; Scott W. Bennett

1994-01-01

103

Ontologies and tag-statistics  

NASA Astrophysics Data System (ADS)

Due to the increasing popularity of collaborative tagging systems, the research on tagged networks, hypergraphs, ontologies, folksonomies and other related concepts is becoming an important interdisciplinary area with great potential and relevance for practical applications. In most collaborative tagging systems the tagging by the users is completely ‘flat’, while in some cases they are allowed to define a shallow hierarchy for their own tags. However, usually no overall hierarchical organization of the tags is given, and one of the interesting challenges of this area is to provide an algorithm generating the ontology of the tags from the available data. In contrast, there are also other types of tagged networks available for research, where the tags are already organized into a directed acyclic graph (DAG), encapsulating the ‘is a sub-category of’ type of hierarchy between each other. In this paper, we study how this DAG affects the statistical distribution of tags on the nodes marked by the tags in various real networks. The motivation for this research was the fact that understanding the tagging based on a known hierarchy can help in revealing the hidden hierarchy of tags in collaborative tagging systems. We analyse the relation between the tag-frequency and the position of the tag in the DAG in two large sub-networks of the English Wikipedia and a protein-protein interaction network. We also study the tag co-occurrence statistics by introducing a two-dimensional (2D) tag-distance distribution preserving both the difference in the levels and the absolute distance in the DAG for the co-occurring pairs of tags. Our most interesting finding is that the local relevance of tags in the DAG (i.e. their rank or significance as characterized by, e.g., the length of the branches starting from them) is much more important than their global distance from the root. Furthermore, we also introduce a simple tagging model based on random walks on the DAG, capable of reproducing the main statistical features of tag co-occurrence. This model has high potential for further practical applications, e.g., it can provide the starting point for a benchmark system in ontology retrieval or it may help pinpoint unusual correlations in the co-occurrence of tags.

Tibély, Gergely; Pollner, Péter; Vicsek, Tamás; Palla, Gergely

2012-05-01

104

Fish Tagging Forum Draft Compilation of Tagging Infrastructure  

E-print Network

Fish Tagging Forum Draft Compilation of Tagging Infrastructure 2012_11_06 v0 One of the items we have been discussing is infrastructure associated with the different tagging/marking technologies. Infrastructure impacts cost considerations as different technologies have different infrastructure requirements

105

Prairie Dog Tagging  

USGS Multimedia Gallery

An anaesthetized prairie dog is tagged in Wind Cave National Park.  Over 30 organizations and agencies are testing a USGS-developed oral vaccine to prevent the spread of plague in prairie dogs. If successful, the sylvatic plague vaccine could help protect endangered black-f...

106

Project 8 Tags  

ERIC Educational Resources Information Center

In this article, the author describes 8 Tags, a project that she used with her eighth-grade studio art students to encourage them to come up with original and creative solutions to an assignment. She also wanted to incorporate their knowledge of the elements of art and principles of design. In this project, students were challenged to create an…

Ramos-Burrows, Michele

2010-01-01

107

Analysis of urinary vitamin D? metabolites by liquid chromatography/tandem mass spectrometry with ESI-enhancing and stable isotope-coded derivatization.  

PubMed

The determination of the urinary vitamin D? metabolites might prove helpful in the assessment of the vitamin D status. We developed a method for the determination of trace vitamin D? metabolites, 25-hydroxyvitamin D? [25(OH)D?] and 24,25-dihydroxyvitamin D? [24,25(OH)?D?], in urine using liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) combined with derivatization using an ESI-enhancing reagent, 4-(4'-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD), and its isotope-coded analogue, (2)H4-DAPTAD (d-DAPTAD). The urine samples were treated with ?-glucuronidase, purified with an Oasis hydrophilic-lipophilic balanced (HLB) cartridge, and then subjected to the derivatization. The DAPTAD derivatization enabled the highly sensitive detection (detection limit, 0.25 fmol on the column), and the use of d-DAPTAD significantly improved the assay precision [the intra- (n?=?5) and inter-assay (n?=?3) relative standard deviations did not exceed 9.5%]. The method was successfully applied to urine sample analyses and detected the increases of the urinary 25(OH)D? and 24,25(OH)?D? levels due to vitamin D? administration. PMID:25168117

Ogawa, Shoujiro; Ooki, Satoshi; Shinoda, Kenta; Higashi, Tatsuya

2014-10-01

108

Enantioselective determination of ibuprofen in saliva by liquid chromatography/tandem mass spectrometry with chiral electrospray ionization-enhancing and stable isotope-coded derivatization.  

PubMed

A method was developed and validated for the enantioselective determination of trace ibuprofen (IBU) in saliva using liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) combined with the derivatization using a chiral ESI-enhancing reagent, (S)-1-(4-dimethylaminophenylcarbonyl)-3-aminopyrrolidine (DAPAP), and its isotope-coded analog, (2)H4-DAPAP (d-DAPAP). The DAPAP-derivatization enabled the highly sensitive detection [detection limit, 0.15fmol (equivalent to 30fg of intact IBU) on the column] and complete separation (resolution 3.1) of the IBU enantiomers. The use of d-DAPAP significantly improved the assay precision and accuracy; the intra- (n=5) and inter-assay (n=5) relative standard deviations did not exceed 6.2%, and good accuracy (101.3-106.1%) was obtained. The developed method was successfully applied to the quantitative analysis of IBU in saliva. Using this method, salivary concentration-time profiles of each enantiomer after a single oral administration of the racemic IBU to healthy subjects were obtained. The area under the salivary concentration-time curve of the (S)-enantiomer was ca. twice that of the (R)-enantiomer due to the unidirectional chiral inversion of the (R)- to (S)-enantiomer in vivo. Thus, saliva-based noninvasive pharmacokinetic analyses of IBU enantiomers were achieved by this method. PMID:24999866

Ogawa, Shoujiro; Tadokoro, Hiroaki; Sato, Maho; Higashi, Tatsuya

2014-09-01

109

Quantitative isomer-specific N-glycan fingerprinting using isotope coded labeling and high performance liquid chromatography-electrospray ionization-mass spectrometry with graphitic carbon stationary phase.  

PubMed

Glycan reductive isotope labeling (GRIL) using (12)C6-/(13)C6-aniline as labeling reagent is reported with the aim of quantitative N-glycan fingerprinting. Porous graphitized carbon (PGC) as stationary phase in capillary scale HPLC coupled to electrospray mass spectrometry with time of flight analyzer was applied for the determination of labeled N-glycans released from glycoproteins. The main benefit of using stable isotope-coding in the context of comparative glycomics lies in the improved accuracy and precision of the quantitative analysis in combined samples and in the potential of correcting for structure-dependent incomplete enzymatic release of oligosaccharides when comparing identical target proteins. The method was validated with respect to mobile phase parameters, reproducibility, accuracy, linearity and limit of detection/quantification (LOD/LOQ) using test glycoproteins. It is shown that the developed method is capable of determining relative amounts of N-glycans (including isomers) comparing two samples in one single HPLC-MS run. The analytical potential and usefulness of GRIL in combination with PGC-ESI-TOF-MS is demonstrated comparing glycosylation in human monoclonal antibodies produced in Chinese hamster ovary cells (CHO) and hybridoma cell lines. PMID:25638265

Michael, Claudia; Rizzi, Andreas M

2015-02-27

110

Expedient synthesis of a modular phosphate affinity reagent.  

PubMed

Isolation and identification of phosphorylated macromolecules is essential for the deconvolution of most biological regulatory networks. Koike and co-workers recently reported the application of a dinuclear zinc-(pyridylmethyl)amine complex to phosphate-specific affinity purifications and gave it the shorthand name "phos-tag". This complex is valuable for studying phosphorylation because it binds selectively to phosphate dianion in the presence of acidic functional groups at physiological pH, and because the binding is largely independent of molecular context. These properties of phos-tag recommend it for applications in phosphoproteomics, metabolomics, and nucleic acid biology. The catch has been that the molecule is difficult to make and prohibitively expensive to buy. Here, we describe an efficient and inexpensive synthesis of a phos-tag derivative with a versatile alkyne handle. The alkyne handle allows for attachment of phos-tag to alkyl azides via the copper(I)-catalyzed azide-alkyne cycloaddition reaction ("click chemistry"). We characterize the phosphate binding behavior of the new phos-tag derivative in a variety of experimental assays, including its conjugation to a fluorescent reporter, to acrylamide gels, and to sepharose chromatography resin. The synthesis we report should enable a broader use of phos-tag for phosphate-related biochemistry, as both an analytical and a preparative reagent. PMID:20491467

Tilmans, Nicolas P; Krusemark, Casey J; Harbury, Pehr A B

2010-06-16

111

His6 tag-assisted chemical protein synthesis  

NASA Astrophysics Data System (ADS)

To make more practical the total chemical synthesis of proteins by the ligation of unprotected peptide building blocks, we have developed a method to facilitate the isolation and handling of intermediate products. The synthetic technique makes use of a His6 tag at the C terminus of the target polypeptide chain, introduced during the synthesis of the C-terminal peptide segment building block. The presence of a His6 tag enables the isolation of peptide or protein products directly from ligation reaction mixtures by Ni-NTA affinity column purification. This simple approach enables facile buffer exchange to alternate reaction conditions and is compatible with direct analytical control by protein MS of the multiple ligation steps involved in protein synthesis. We used syntheses of crambin and a modular tetratricopeptide repeat protein of 17 kDa as models to examine the utility of this affinity purification approach. The results show that His6 tag-assisted chemical protein synthesis is a useful method that substantially reduces handling losses and provides for rapid chemical protein syntheses. affinity purification | native chemical ligation

Bang, Duhee; Kent, Stephen B. H.

2005-04-01

112

Tag Retention of T-Bar Anchor Tags and Passive Integrated Transponder Tags in Shovelnose Sturgeon  

Microsoft Academic Search

Capture–recapture methods are commonly used to estimate population parameters when the necessary assumptions are met. One of the broadest assumptions of capture–recapture models is that tags are not lost. Therefore, one must understand tag retention to be able to adjust estimates if tag loss occurs. Our objectives were to (1) determine retention rates of T-bar anchor tags and passive integrated

Martin J. Hamel; Jeremy J. Hammen; Mark A. Pegg

2012-01-01

113

Two-Step Affinity Purification System HisStrep pQE-TriSystem Vector Set  

E-print Network

Two-Step Affinity Purification System Handbook His·Strep pQE-TriSystem Vector Set pQE-TriSystem Strep Vector Strep-Tactin® Superflow Strep-Tactin Magnetic Beads Strep-tag® Antibody For expressing, purifying, and detecting proteins carrying a 6xHis and Strep-tag II July 2003 W W W . Q I A G E N . C O M

Lebendiker, Mario

114

TagMyDoc  

NSDL National Science Digital Library

Do you need to share documents quickly with a number of different users? You may want to give TagMyDoc a look. Visitors can choose their documents, and upload them so they can be scanned and retrieved as virtual copies. Additionally, users can sign up for free accounts for enhanced functionality and there's an explanatory video here as well. This version is compatible with all operating systems.

2012-01-01

115

Inter-Thread Affinity Analysis  

Microsoft Academic Search

This paper explores inter-thread affinity of OpenMP paral- lel programs to aid in data reuse for increasing cache per- formance. We build upon the Cetus source to source com- piler and utilize existing Cetus abilities to ultimately repre- sent thread affinity numerically. General Terms inter-thread affinity, affinity analysis, Ex- ploring Parallel IR, Cetus, Cetus HIR

Derrin Pierret; Christoph Frei

2008-01-01

116

Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme.  

PubMed

Transient transfection of mammalian cells has proven to be a useful technique for the rapid production of recombinant proteins because of its ability to produce milligram quantities within 2 weeks following cloning of their corresponding cDNA. This rapid production also requires a fast and efficient purification scheme that can be applied generically, typically through the use of affinity tags such as the polyhistidine-tag for capture by immobilized metal-affinity chromatography (IMAC) or the Strep-tag II, which binds to the StrepTactin affinity ligand. However, one-step purification using either of these tags has disadvantages in terms of yield, elution conditions, and purity. Here, we show that the addition of both Strep-tag-II and (His)(8) to the C-terminal of r-proteins allows efficient purification by consecutive IMAC and StrepTactin affinity. This approach has been successfully demonstrated using the intracellular protein DsRed, as well as two secreted proteins, secreted alkaline phosphatase (SEAP) and vascular endothelial growth factor (VEGF), all produced by transient transfection of HEK293-EBNA1 cells in medium supplemented with bovine calf serum. All proteins were purified to >99% homogeneity with yields varying from 29 to 81%. PMID:15721774

Cass, Brian; Pham, Phuong Lan; Kamen, Amine; Durocher, Yves

2005-03-01

117

Social Tagging of Mission Data  

NASA Technical Reports Server (NTRS)

Mars missions will generate a large amount of data in various forms, such as daily plans, images, and scientific information. Often, there is a semantic linkage between images that cannot be captured automatically. Software is needed that will provide a method for creating arbitrary tags for this mission data so that items with a similar tag can be related to each other. The tags should be visible and searchable for all users. A new routine was written to offer a new and more flexible search option over previous applications. This software allows users of the MSLICE program to apply any number of arbitrary tags to a piece of mission data through a MSLICE search interface. The application of tags creates relationships between data that did not previously exist. These tags can be easily removed and changed, and contain enough flexibility to be specifically configured for any mission. This gives users the ability to quickly recall or draw attention to particular pieces of mission data, for example: Give a semantic and meaningful description to mission data; for example, tag all images with a rock in them with the tag "rock." Rapidly recall specific and useful pieces of data; for example, tag a plan as"driving template." Call specific data to a user s attention; for example, tag a plan as "for:User." This software is part of the MSLICE release, which was written in Java. It will run on any current Windows, Macintosh, or Linux system.

Norris, Jeffrey S.; Wallick, Michael N.; Joswig, Joseph C.; Powell, Mark W.; Torres, Recaredo J.; Mittman, David S.; Abramyan, Lucy; Crockett, Thomas M.; Shams, Khawaja S.; Fox, Jason M.; Pyrzak, Guy; Vaughn, Michael B.

2010-01-01

118

Affine Structure and Photometry  

Microsoft Academic Search

Motion of an observer relative to objects in a scene provides information about the structure of the scene. Changing patterns of shading due to motion relative to the light source provide information about surface structure, albedos, and light sources. One can stratify this photometric information into affine, unitary, and metric structure, much like the stratification of structure from motion. For

Ruth Rosenholtz; Jan J. Koenderink

1996-01-01

119

Fluorescence: Molecular Tagging  

NSDL National Science Digital Library

In this activity, by the Concord Consortium's Molecular Literacy project, students will be introduced to the concept of fluorescence and how this method allows scientists to better analyze objects at a molecular level. The site is interactive and allows students to interact with virtual cells and tag them just as a scientist would. The focus of the topic can be applied to many different fields, such as: mineralogy, biochemistry, medicine, forensics and biotechnology. The activity itself is a java-based interactive resource built upon the free, open source Molecular Workbench software. In addition, visitors will find an overview of the activity, assessments, and concepts and their correlation to AAAS and NSES standards.

120

Structure of classical affine and classical affine fractional W -algebras  

NASA Astrophysics Data System (ADS)

We introduce a classical BRST complex (See Definition 3.2.) and show that one can construct a classical affine W -algebra via the complex. This definition clarifies that classical affine W -algebras can be considered as quasi-classical limits of quantum affine W -algebras. We also give a definition of a classical affine fractional W -algebra as a Poisson vertex algebra. As in the classical affine case, a classical affine fractional W -algebra has two compatible ?-brackets and is isomorphic to an algebra of differential polynomials as a differential algebra. When a classical affine fractional W -algebra is associated to a minimal nilpotent, we describe explicit forms of free generators and compute ?-brackets between them. Provided some assumptions on a classical affine fractional W -algebra, we find an infinite sequence of integrable systems related to the algebra, using the generalized Drinfel'd and Sokolov reduction.

Suh, Uhi Rinn

2015-01-01

121

Development of a Dehalogenase-Based Protein Fusion Tag Capable of Rapid, Selective and Covalent Attachment to Customizable Ligands  

PubMed Central

Our fundamental understanding of proteins and their biological significance has been enhanced by genetic fusion tags, as they provide a convenient method for introducing unique properties to proteins so that they can be examinedin isolation. Commonly used tags satisfy many of the requirements for applications relating to the detection and isolation of proteins from complex samples. However, their utility at low concentration becomes compromised if the binding affinity for a detection or capture reagent is not adequate to produce a stable interaction. Here, we describe HaloTag® (HT7), a genetic fusion tag based on a modified haloalkane dehalogenase designed and engineered to overcome the limitation of affinity tags by forming a high affinity, covalent attachment to a binding ligand. HT7 and its ligand have additional desirable features. The tag is relatively small, monomeric, and structurally compatible with fusion partners, while the ligand is specific, chemically simple, and amenable to modular synthetic design. Taken together, the design features and molecular evolution of HT7 have resulted in a superior alternative to common tags for the overexpression, detection, and isolation of target proteins. PMID:23248739

Encell, Lance P; Friedman Ohana, Rachel; Zimmerman, Kris; Otto, Paul; Vidugiris, Gediminas; Wood, Monika G; Los, Georgyi V; McDougall, Mark G; Zimprich, Chad; Karassina, Natasha; Learish, Randall D; Hurst, Robin; Hartnett, James; Wheeler, Sarah; Stecha, Pete; English, Jami; Zhao, Kate; Mendez, Jacqui; Benink, Hélène A; Murphy, Nancy; Daniels, Danette L; Slater, Michael R; Urh, Marjeta; Darzins, Aldis; Klaubert, Dieter H; Bulleit, Robert F; Wood, Keith V

2012-01-01

122

Efficient techniques for monitoring missing RFID tags  

Microsoft Academic Search

As RFID tags become more widespread, new approaches for managing larger numbers of RFID tags will be needed. In this paper, we consider the problem of how to accurately and efficiently monitor a set of RFID tags for missing tags. Our approach accurately monitors a set of tags without collecting IDs from them. It differs from traditional research which focuses

Chiu Chiang Tan; Bo Sheng; Qun Li

2010-01-01

123

Analysis of tag within online social networks  

Microsoft Academic Search

In recent years, tagging systems have been paid increasing attentions from both research communities and system designers. Most popular online social networking sites harness tag for managing and locating contents, for organizing and connecting users, and for recommending and sharing resources. We believe that tag acts like bridge between people and resources. Research on tag and tagging behavior will provide

Chao Wu; Bo Zhou

2009-01-01

124

Cobalt carbonyl complexes as probes for alkyne-tagged lipids.  

PubMed

Monitoring lipid distribution and metabolism in cells and biological fluids poses many challenges because of the many molecular species and metabolic pathways that exist. This study describes the synthesis and study of molecules that contain an alkyne functional group as surrogates for natural lipids in cultured cells. Thus, hexadec-15-ynoic and hexadec-7-ynoic acids were readily incorporated into RAW 264.7 cells, principally as phosphocholine esters; the alkyne was used as a "tag" that could be transformed to a stable dicobalt-hexacarbonyl complex; and the complex could then be detected by HPLC/MS or HPLC/UV(349nm). The 349 nm absorbance of the cobalt complexes was used to provide qualitative and quantitative information about the distribution and cellular concentrations of the alkyne lipids. The alkyne group could also be used as an affinity tag for the lipids by a catch-and-release strategy on phosphine-coated silica beads. Lipid extracts were enriched in the tagged lipids in this way, making the approach of potential utility to study lipid transformations in cell culture. Both terminal alkynes and internal alkynes were used in this affinity "pull-down" strategy. This method facilitates measuring lipid species that might otherwise fall below limits of detection. PMID:23307946

Tallman, Keri A; Armstrong, Michelle D; Milne, Stephen B; Marnett, Lawrence J; Brown, H Alex; Porter, Ned A

2013-03-01

125

ChemTeacher: Electron Affinity  

NSDL National Science Digital Library

ChemTeacher compiles background information, videos, articles, demonstrations, worksheets and activities for high school teachers to use in their classrooms. The Electron Affinity page includes resources for teaching students about the concept of electron affinity.

2012-07-20

126

Epitope tagging of endogenous proteins  

E-print Network

-in'. The tagging method is straightforward, can be applied to many loci and several human somatic cell lines contains two multiple cloning sites, sequences that encode a triple Flag epitope tag (3Ã?Flag), a neomycin respective cloning sites, and packaged the resulting vector into recombinant adeno-associated virus (r

Weng, Zhiping

127

Affine Patches on Positroid Varieties and Affine Pipe Dreams (Thesis)  

E-print Network

The objects of interest in this thesis are positroid varieties in the Grassmannian, which are indexed by juggling patterns. In particular, we study affine patches on these positroid varieties. Our main result corresponds these affine patches to Kazhdan-Lusztig varieties in the affine Grassmannian. We develop a new term order and study how these spaces are related to subword complexes and Stanley-Reisner ideals. We define an extension of pipe dreams to the affine case and conclude by showing how our affine pipe dreams are generalizations of Cauchon and Le diagrams.

Snider, Michelle

2010-01-01

128

Affinity driven social networks  

NASA Astrophysics Data System (ADS)

In this work we present a model for evolving networks, where the driven force is related to the social affinity between individuals of a population. In the model, a set of individuals initially arranged on a regular ordered network and thus linked with their closest neighbors are allowed to rearrange their connections according to a dynamics closely related to that of the stable marriage problem. We show that the behavior of some topological properties of the resulting networks follows a non trivial pattern.

Ruyú, B.; Kuperman, M. N.

2007-04-01

129

Near infrared multifactor identification tags.  

PubMed

We propose a compact technique for encryption-verification that relies on the following elements: multifactor encryption, which permits the simultaneous verification of up to four factors; distortion-invariant ID tag for remote identification; near infrared (NIR) writing and readout of the ID tag signal for invisible transmission; and optical processor, based on joint transform pattern recognition by optical correlation, for automatic verification of information. A highly-reliable security system is obtained by joining the advantages of all these elements for the first time. A novel NIR ID tag is designed and built by using commonly available materials. The very ID tag content cannot be visually perceived at naked eye; it cannot be either copied, scanned, or captured by any conventional device. Experimental results based on the NIR ID tag are shown. The satisfactory results obtained demonstrate a new insight into the applications of the compact and efficient technique for high-secure identification systems. PMID:19550849

Pérez-Cabré, Elisabet; Millán, Maria S; Javidi, Bahram

2007-11-12

130

Quantized Affinely Rigid Bodies  

E-print Network

Developed are the main ideas of the quantized version of affinely-rigid motion. We base on the usual Schr\\"odinger formulation of quantum mechanics in the configuration manifold. The latter is given, in our case, by the affine group or equivalently by the semi-direct product of the linear group ${\\rm GL}(n,\\mathbb{R})$ and the space of translations $\\mathbb{R}^{n}$, where $n$ equals the dimension of the "physical space". In particular, we discuss the problem of the dynamical invariance of the kinetic energy under the action of affine group, not only under the isometry subgroup. Technically, the treatment is based on the two-polar decomposition of the matrix of the internal configuration space and on the Peter-Weyl theory of generalized Fourier series on Lie groups. One can hope that our results may be applied in quantum problems of nuclear dynamics or even in apparently exotic phenomena in vibrating neutron stars. And, of course, some more prosaic applications in molecular dynamics are possible.

J. J. S\\lawianowski; B. Go\\lubowska; V. Kovalchuk; A. Martens; E. E. Ro?ko

2014-05-12

131

Expression of a Translationally Fused TAP-Tagged Plasma Membrane Proton Pump in Arabidopsis thaliana  

PubMed Central

The Arabidopsis thaliana plasma membrane proton ATPase genes, AHA1 and AHA2, are the two most highly expressed isoforms of an 11 gene family and are collectively essential for embryo development. We report the translational fusion of a tandem affinity-purification tag to the 5? end of the AHA1 open reading frame in a genomic clone. Stable expression of TAP-tagged AHA1 in Arabidopsis rescues the embryonic lethal phenotype of endogenous double aha1/aha2 knockdowns. Western blots of SDS-PAGE and Blue Native gels show enrichment of AHA1 in plasma membrane fractions and indicate a hexameric quaternary structure. TAP-tagged AHA1 rescue lines exhibited reduced vertical root growth. Analysis of the plasma membrane and soluble proteomes identified several plasma membrane-localized proteins with alterred abundance in TAP-tagged AHA1 rescue lines compared to wild type. Using affinity-purification mass spectrometry, we uniquely identified two additional AHA isoforms, AHA9 and AHA11, which copurified with TAP-tagged AHA1. In conclusion, we have generated transgenic Arabidopsis lines in which a TAP-tagged AHA1 transgene has complemented all essential endogenous AHA1 and AHA2 functions and have shown that these plants can be used to purify AHA1 protein and to identify in planta interacting proteins by mass spectrometry. PMID:24397334

2015-01-01

132

A repertoire of peptide tags for controlled drug release from injectable noncovalent hydrogel.  

PubMed

A repertoire of conjugable tags for controlling the release of drugs from biomaterials is highly interesting for the development of combinatorial drug administration techniques. This paper describes such a system of 11 peptide tags derived from our previous work on a physical hydrogel system cross-linked through peptide-heparin interactions. The release kinetics of the tags correlate well with their affinity to heparin and obey Fick's second law of diffusion, with the exception of the ATIII peptide, which displays a stable release profile close to a zero-order reaction. A system for release experiments over seven months was built, using the hydrogel matrix as a barrier between the reservoirs of tagged compounds and supernatant. The gel matrix can be injected without affecting the releasing properties. A tagged cyclosporin A derivative was also tested, and its release was monitored by measuring its biological activity. This work represents a design of biomaterials with an integral system of drug delivery, where both the assembly process of the matrix and affinity capture/release of tagged compounds are based on the noncovalent interaction of heparin with one class of peptides. PMID:24825401

Wieduwild, Robert; Lin, Weilin; Boden, Annett; Kretschmer, Karsten; Zhang, Yixin

2014-06-01

133

Dimerization Capacities of FGF2 Purified with or without Heparin-Affinity Chromatography  

PubMed Central

Fibroblast growth factor-2 (FGF2) is a pleiotropic growth factor exhibiting a variety of biological activities. In this article, we studied the capacity of FGF2 purified with or without heparin affinity chromatography to self-associate. Analyzing the NMR HSQC spectra for different FGF2 concentrations, heparin-affinity purified FGF2 showed perturbations that indicate dimerization and are a higher-order oligomerization state. HSQC perturbation observed with different FGF2 concentrations revealed a heparin-binding site and two dimer interfaces. Thus, with increasing protein concentrations, FGF2 monomers make contacts with each other and form dimers or higher order oligomers. On the contrary, FGF2 purified with ion-exchange chromatography did not show similar perturbation indicating that self-association of FGF2 is eliminated if purification is done without heparin-affinity chromatography. The HSQC spectra of heparin-affinity purified FGF2 can be reproduced to some extent by adding heparin tetra-saccharide to ion exchange chromatography purified FGF2. Heparin-affinity purified FGF2 bound to acceptor and donor beads in a tagged form using His-tagged or GST-tagged proteins, also dimerized in the AlphaScreen™ assay. This assay was further validated using different experimental conditions and competitors. The assay constitutes an interesting tool to study dimerization of other FGF forms as well. PMID:25299071

Chiu, Liang-Yuan; Taouji, Said; Moroni, Elisabetta; Colombo, Giorgio; Chevet, Eric; Sue, Shih-Che; Bikfalvi, Andreas

2014-01-01

134

Dimerization capacities of FGF2 purified with or without heparin-affinity chromatography.  

PubMed

Fibroblast growth factor-2 (FGF2) is a pleiotropic growth factor exhibiting a variety of biological activities. In this article, we studied the capacity of FGF2 purified with or without heparin affinity chromatography to self-associate. Analyzing the NMR HSQC spectra for different FGF2 concentrations, heparin-affinity purified FGF2 showed perturbations that indicate dimerization and are a higher-order oligomerization state. HSQC perturbation observed with different FGF2 concentrations revealed a heparin-binding site and two dimer interfaces. Thus, with increasing protein concentrations, FGF2 monomers make contacts with each other and form dimers or higher order oligomers. On the contrary, FGF2 purified with ion-exchange chromatography did not show similar perturbation indicating that self-association of FGF2 is eliminated if purification is done without heparin-affinity chromatography. The HSQC spectra of heparin-affinity purified FGF2 can be reproduced to some extent by adding heparin tetra-saccharide to ion exchange chromatography purified FGF2. Heparin-affinity purified FGF2 bound to acceptor and donor beads in a tagged form using His-tagged or GST-tagged proteins, also dimerized in the AlphaScreen™ assay. This assay was further validated using different experimental conditions and competitors. The assay constitutes an interesting tool to study dimerization of other FGF forms as well. PMID:25299071

Platonova, Natalia; Miquel, Géraldine; Chiu, Liang-Yuan; Taouji, Said; Moroni, Elisabetta; Colombo, Giorgio; Chevet, Eric; Sue, Shih-Che; Bikfalvi, Andreas

2014-01-01

135

Quantum tagging for tags containing secret classical data  

SciTech Connect

Various authors have considered schemes for quantum tagging, that is, authenticating the classical location of a classical tagging device by sending and receiving quantum signals from suitably located distant sites, in an environment controlled by an adversary whose quantum information processing and transmitting power is potentially unbounded. All of the schemes proposed elsewhere in the literature assume that the adversary is able to inspect the interior of the tagging device. All of these schemes have been shown to be breakable if the adversary has unbounded predistributed entanglement. We consider here the case in which the tagging device contains a finite key string shared with distant sites but kept secret from the adversary, and show this allows the location of the tagging device to be authenticated securely and indefinitely. Our protocol relies on quantum key distribution between the tagging device and at least one distant site, and demonstrates a new practical application of quantum key distribution. It also illustrates that the attainable security in position-based cryptography can depend crucially on apparently subtle details in the security scenario considered.

Kent, Adrian [Centre for Quantum Information and Foundations, DAMTP, University of Cambridge, Cambridge (United Kingdom) and Perimeter Institute for Theoretical Physics, Waterloo, Ontario (Canada)

2011-08-15

136

Tagging insulin in microgravity  

NASA Technical Reports Server (NTRS)

Knowing the exact subcellular sites of action of insulin in the body has the potential to give basic science investigators a basis from which a cause and cure for this disease can be approached. The goal of this project is to create a test reagent that can be used to visualize these subcellular sites. The unique microgravity environment of the Shuttle will allow the creation of a reagent that has the possibility of elucidating the subcellular sites of action of insulin. Several techniques have been used in an attempt to isolate the sites of action of items such as insulin. One of these is autoradiography in which the test item is obtained from animals fed radioactive materials. What is clearly needed is to visualize individual insulin molecules at their sites of action. The insulin tagging process to be used on G-399 involves the conjugation of insulin molecules with ferritin molecules to create a reagent that will be used back on Earth in an attempt to elucidate the sites of action of insulin.

Dobeck, Michael; Nelson, Ronald S.

1992-01-01

137

Efficient memoryless protocol for tag identification  

Microsoft Academic Search

This paper presents an efficient collision resolution protocol and its variations for the tag identification problem, where an electromagnetic reader attempts to obtain within is read range the unique ID number of each tag. The novelty of our main protocol is that each tag is memoryless, i.e., the current response of each tag only depends on the current query of

Ching Law; Kayi Lee; Kai-yeung Siu

2000-01-01

138

A low-cost affinity purification system using ?-1,3-glucan recognition protein and curdlan beads.  

PubMed

Silkworm ?-1,3-glucan recognition protein (?GRP) tightly and specifically associates with ?-1,3-glucan. We report here an affinity purification system named the 'GRP system', which uses the association between the ?-1,3-glucan recognition domain of ?GRP (GRP-tag), as an affinity tag, and curdlan beads. Curdlan is a water-insoluble ?-1,3-glucan reagent, the low cost of which (about 100 JPY/g) allows the economical preparation of beads. Curdlan beads can be readily prepared by solubilization in an alkaline solution, followed by neutralization, sonication and centrifugation. We applied the GRP system to preparation of several proteins and revealed that the expression levels of the GRP-tagged proteins in soluble fractions were two or three times higher than those of the glutathione S-transferase (GST)-tagged proteins. The purity of the GRP-tagged proteins on the curdlan beads was comparable to that of the GST-tagged proteins on glutathione beads. The chemical stability of the GRP system was more robust than conventional affinity systems under various conditions, including low pH (4-6). Biochemical and structural analyses revealed that proteins produced using the GRP system were structurally and functionally active. Thus, the GRP system is suitable for both the large- and small-scale preparation of recombinant proteins for functional and structural analyses. PMID:22706764

Horiuchi, Masataka; Takahasi, Kiyohiro; Kobashigawa, Yoshihiro; Ochiai, Masanori; Inagaki, Fuyuhiko

2012-08-01

139

TAG Based Skimming In ATLAS  

NASA Astrophysics Data System (ADS)

The ATLAS detector at the LHC takes data at 200-500 Hz for several months per year accumulating billions of events for hundreds of physics analyses. TAGs are event-level metadata allowing a quick search for interesting events based on selection criteria defined by the user. They are stored in a file-based format as well as in relational databases. The overall TAG system architecture encompasses a range of interconnected services that provide functionality for the required use cases such as event selection, display, extraction and skimming. Skimming can be used to navigate to any of the pre-TAG data products. The services described in this paper address use cases that range in scale from selecting a handful of interesting events for an analysis specific study to creating physics working group samples on the ATLAS production system. This paper will focus on the workflow aspects involved in creating pre and post TAG data products from a TAG selection using the Grid in the context of the overall TAG system architecture. The emphasis will be on the range of demands that the implemented use cases place on these workflows and on the infrastructure. The tradeoffs of various workflow strategies will be discussed including scalability issues and other concerns that occur when integrating with data management and production systems.

Doherty, T.; Cranshaw, J.; Hrivnac, J.; Slater, M.; Nowak, M.; Quilty, D.; Zhang, Q.

2012-12-01

140

Scalable Grouping-proof Protocol for RFID Tags Grouping-Proof Protocol for RFID Tags  

E-print Network

Scalable Grouping-proof Protocol for RFID Tags Grouping-Proof Protocol for RFID Tags: Security-proof protocol for RFID tags based on secret sharing. Our proposed protocol addresses the scalability issue of the previous protocols by removing the need for an RFID reader to relay messages from one tag to another tag

141

Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics  

E-print Network

The abbreviations used are: SILAC: Stable isotope labeling by amino acids in cell culture, 2DE: two dimensional (isoelectric focusing/SDS-PAGE) gel electrophoresis: ICATTM: isotope-coded affinity tag; MS: mass spectrometry; MALDI-TOF: matrix assisted laser desorption ionization-time of flight; PMF

Shao-en Ong; Blagoy Blagoev; Irina Kratchmarova; Dan Bach Kristensen; Akhilesh P; Matthias Mann

2002-01-01

142

A streamlined platform for high-content functional proteomics of primary human specimens  

E-print Network

-MS; for example, isotope-coded affinity tagging (ICAT)4 and multidimensional- protein identification technology of these approaches reveals an intriguing paradox. Multidimen- sional separation methods have remarkable sensitivity that have greater throughput (matrix-assisted laser desorption ionization (MALDI) imaging6 and surface

Cai, Long

143

From the Cover: Imaging of receptor trafficking by using -bungarotoxin-binding-site-tagged receptors  

NASA Astrophysics Data System (ADS)

-Amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA) receptors mediate excitatory synaptic transmission and are dynamically regulated during synaptic plasticity in the CNS. The membrane trafficking of AMPA receptors to synapses is critical for the regulation of the efficacy of excitatory synaptic transmission. Direct imaging of AMPA receptors in various cell compartments is important to dissecting the regulation of distinct steps in receptor membrane trafficking. In this study, we have developed an approach for the imaging of receptor trafficking with subunits tagged with a 13-aa -bungarotoxin (BTX)-binding site (BBS). The small polypeptide neurotoxin BTX has been used for decades to study the nicotinic acetylcholine receptor. Similar high-affinity ligands are rarely available for most receptors. Engineering the BBS tag into receptor subunits allowed the high-affinity binding of fluorescent, radioactive, and biotinylated BTX to the tagged receptor subunits. By using this approach, the total receptor expression, surface expression, internalization, and insertion of receptors into the plasma membrane could be visualized and quantified in fixed or live cells including cultured neurons. The BBS tag is a flexible approach for labeling membrane proteins and studying their dynamic trafficking. GFP | glutamate receptor | live imaging | synapses | tag

Sekine-Aizawa, Yoko; Huganir, Richard L.

2004-12-01

144

Structural and functional characterization of a new recombinant histidine-tagged acyl coenzyme A binding protein (ACBP) from mouse.  

PubMed

Acyl coenzyme A binding protein (ACBP) has been proposed to transport fatty acyl CoAs intracellularly, facilitating their metabolism. In this study, a new mouse recombinant ACBP was produced by insertion of a histidine (his) tag at the C-terminus to allow efficient purification by Ni-affinity chromatography. The his-tag was inserted at the C-terminus since ACBP is a small molecular size (10 kDa) protein whose structure and activity are sensitive to amino acid substitutions in the N-terminus. The his-tag had no or little effect on ACBP structure or ligand binding affinity and specificity. His-ACBP bound the naturally occurring fluorescent cis-parinaroyl-CoA with very high affinity (K(d)=2.15 nM), but exhibited no affinity for non-esterified cis-parinaric acid. To determine if the presence of the C-terminal his-tag altered ACBP interactions with other proteins, direct binding to hepatocyte nuclear factor-4alpha (HNF-4alpha), a nuclear receptor regulating transcription of genes involved in lipid metabolism, was examined. His-ACBP and HNF-4alpha were labeled with Cy5 and Cy3, respectively, and direct interaction was determined by a novel fluorescence resonance energy transfer (FRET) binding assay. FRET analysis showed that his-ACBP directly interacted with HNF-4alpha (intermolecular distance of 73 A) at high affinity (K(d)=64-111 nM) similar to native ACBP. The his-tag also had no effect on ACBPs ability to interact with and stimulate microsomal enzymes utilizing or forming fatty acyl CoA. Thus, C-terminal his-tagged-ACBP maintained very similar structural and functional features of the untagged native protein and can be used in further in vitro experiments that require pure recombinant ACBP. PMID:18178100

Petrescu, Anca D; Huang, Huan; Hostetler, Heather A; Schroeder, Friedhelm; Kier, Ann B

2008-04-01

145

Isolation and purification of blood group antigens using immuno-affinity chromatography on short monolithic columns.  

PubMed

Monolithic columns have gained increasing attention as stationary phases for the separation of biomolecules and biopharmaceuticals. In the present work the performance of monolithic convective interaction media (CIM(®)) chromatography for the purification of blood group antigens was established. The proteins employed in this study are derived from blood group antigens Knops, JMH and Scianna, equipped both with a His-tag and with a V5-tag by which they can be purified. In a first step a monoclonal antibody directed against the V5-tag was immobilized on a CIM(®) Disk with epoxy chemistry. After this, the immobilized CIM(®) Disk was used in immuno-affinity chromatography to purify the three blood group antigens from cell culture supernatant. Up-scaling of the applied technology was carried out using CIM(®) Tubes. In comparison to conventional affinity chromatography, blood group antigens were also purified via His-tag using a HiTrap(®) metal-affinity column. The two purifications have been compared regarding purity, yield and purification speed. Using the monolithic support, it was possible to isolate the blood group antigens with a higher flow rate than using the conventional bed-packed column. PMID:21194702

Mönster, Andrea; Hiller, Oliver; Grüger, Daniela; Blasczyk, Rainer; Kasper, Cornelia

2011-02-01

146

Agilent Human 14 Multiple Affinity  

E-print Network

, IgG, antitrypsin, IgA, transferrin, hapto- globin, fibrinogen, alpha2-macroglobulin, alpha1-acid: Albumin, IgG, Antitrypsin, IgA, Transferrin, Haptoglobin, Fibrinogen, Alpha2-Macroglobulin, Alpha1-Acid, affinity column 10 Ã? 100 mm, affinity column Remove human albumin, IgG, antitrypsin, IgA, transferrin

Lebendiker, Mario

147

Creation and biophysical characterization of a high-affinity, monomeric EGF receptor ectodomain using fluorescent proteins.  

PubMed

X-ray structural studies revealed two conformations of the epidermal growth factor receptor (EGFR) ectodomain (ECD): a compact, tethered conformation in the absence of EGF and an untethered or extended conformation in the presence of EGF. An EGFR-ECD derivative with a monomeric red fluorescent protein (mRFP) at the N-terminus and an enhanced green fluorescent protein (eGFP) at the C-terminus (dual-tag-EGFR-ECD) was created and characterized. The dual-tag-EGFR-ECD construct was shown to have high affinity (nanomolar range) for both EGF and EGFR monoclonal antibody (mAb528). The dual-tag-EGFR-ECD was further characterized by fluorescence-detected analytical ultracentrifugation, lifetime FRET, and fluorescence anisotropy. We found no evidence of a tethered unliganded conformation, nor did we observe a large shape change upon ligand binding as predicted by the crystal models. Increases in steady-state anisotropy upon binding of EGF to the dual-tag-EGFR-ECD were observed and interpreted as changes in the protein flexibility and dynamics. We conclude the fluorescent protein tags perturb the EGFR-ECD structure, making it extended with a 50-fold higher affinity for EGF relative to that of the nontagged EGFR-ECD. PMID:20715761

Kozer, Noga; Henderson, Christine; Bailey, Michael F; Rothacker, Julie; Nice, Edouard C; Burgess, Anthony W; Clayton, Andrew H A

2010-09-01

148

Social image tagging with diverse semantics.  

PubMed

We have witnessed the popularity of image-sharing websites for sharing personal experiences through photos on the Web. These websites allow users describing the content of their uploaded images with a set of tags. Those user-annotated tags are often noisy and biased. Social image tagging aims at removing noisy tags and suggests new relevant tags. However, most existing tag enrichment approaches predominantly focus on tag relevance and overlook tag diversity problem. How to make the top-ranked tags covering a wide range of semantic is still an opening, yet challenging, issue. In this paper, we propose an approach to retag social images with diverse semantics. Both the relevance of a tag to image as well as its semantic compensations to the already determined tags are fused to determine the final tag list for a given image. Different from existing image tagging approaches, the top-ranked tags are not only highly relevant to the image but also have significant semantic compensations with each other. Experiments show the effectiveness of the proposed approach. PMID:25415950

Qian, Xueming; Hua, Xian-Sheng; Tang, Yuan Yan; Mei, Tao

2014-12-01

149

A laboratory evaluation of tagging-related mortality and tag loss in juvenile humpback chub  

USGS Publications Warehouse

We quantified tag retention, survival, and growth in juvenile, captive-reared Humpback Chub Gila cypha marked with three different tag types: (1) Biomark 12.5-mm, 134.2-kHz, full duplex PIT tags injected into the body cavity with a 12-gauge needle; (2) Biomark 8.4-mm, 134.2-kHz, full duplex PIT tags injected with a 16-gauge needle; and (3) Northwest Marine Technology visible implant elastomer (VIE) tags injected under the skin with a 29-gauge needle. Estimates of tag loss, tagging-induced mortality, and growth were evaluated for 60 d with each tag type for four different size-groups of fish: 40–49 mm, 50–59 mm, 60–69 mm, and 70–79 mm TL. Total length was a significant predictor of the probability of PIT tag retention and mortality for both 8-mm and 12-mm PIT tags, and the smallest fish had the highest rates of tag loss (12.5–30.0%) and mortality (7.5–20.0%). Humpback Chub of sizes 40–49 mm TL and tagged with VIE tags had no mortality but did have a 17.5% tag loss. Growth rates of all tagged fish were similar to controls. Our data indicate Humpback Chub can be effectively tagged using either 8-mm or 12-mm PIT tags with little tag loss or mortality at sizes as low as 65 mm TL.

Ward, David L.; Persons, William R.; Young, Kirk; Stone, Dennis M.; Van Haverbeke, Randy; Knight, William R.

2015-01-01

150

Directed supramolecular surface assembly of SNAP-tag fusion proteins.  

PubMed

Supramolecular assembly of proteins on surfaces and vesicles was investigated by site-selective incorporation of a supramolecular guest element on proteins. Fluorescent proteins were site-selectively labeled with bisadamantane by SNAP-tag technology. The assembly of the bisadamantane functionalized SNAP-fusion proteins on cyclodextrin-coated surfaces yielded stable monolayers. The binding of the fusion proteins is specific and occurs with an affinity in the order of 10(6) M(-1) as determined by surface plasmon resonance. Reversible micropatterns of the fusion proteins on micropatterned cyclodextrin surfaces were visualized by using fluorescence microscopy. Furthermore, the guest-functionalized proteins could be assembled out of solution specifically onto the surface of cyclodextrin vesicles. The SNAP-tag labeling of proteins thus allows for assembly of modified proteins through a host-guest interaction on different surfaces. This provides a new strategy in fabricating protein patterns on surfaces and takes advantage of the high labeling efficiency of the SNAP-tag with designed supramolecular elements. PMID:22511333

Uhlenheuer, Dana A; Wasserberg, Dorothee; Haase, Christian; Nguyen, Hoang D; Schenkel, Jan Hendrik; Huskens, Jurriaan; Ravoo, Bart Jan; Jonkheijm, Pascal; Brunsveld, Luc

2012-05-29

151

Monodisperse, "Highly" Positively Charged Protein Polymer Drag-Tags Generated in an Intein-Mediated Purification System Used in  

E-print Network

-Mediated Purification System Used in Free-Solution Electrophoretic Separations of DNA Xiaoxiao Wang,1 Jennifer Coyne engineered, highly repetitive polypeptides ("protein polymers") that are designed to be large, water, a one-step purification method that combines affinity chromatography and on-column tag cleavage

Barron, Annelise E.

152

WebTag: Web Browsing into Sensor Tags over NFC  

PubMed Central

Information and Communication Technologies (ICTs) continue to overcome many of the challenges related to wireless sensor monitoring, such as for example the design of smarter embedded processors, the improvement of the network architectures, the development of efficient communication protocols or the maximization of the life cycle autonomy. This work tries to improve the communication link of the data transmission in wireless sensor monitoring. The upstream communication link is usually based on standard IP technologies, but the downstream side is always masked with the proprietary protocols used for the wireless link (like ZigBee, Bluetooth, RFID, etc.). This work presents a novel solution (WebTag) for a direct IP based access to a sensor tag over the Near Field Communication (NFC) technology for secure applications. WebTag allows a direct web access to the sensor tag by means of a standard web browser, it reads the sensor data, configures the sampling rate and implements IP based security policies. It is, definitely, a new step towards the evolution of the Internet of Things paradigm. PMID:23012511

Echevarria, Juan Jose; Ruiz-de-Garibay, Jonathan; Legarda, Jon; Álvarez, Maite; Ayerbe, Ana; Vazquez, Juan Ignacio

2012-01-01

153

WebTag: Web browsing into sensor tags over NFC.  

PubMed

Information and Communication Technologies (ICTs) continue to overcome many of the challenges related to wireless sensor monitoring, such as for example the design of smarter embedded processors, the improvement of the network architectures, the development of efficient communication protocols or the maximization of the life cycle autonomy. This work tries to improve the communication link of the data transmission in wireless sensor monitoring. The upstream communication link is usually based on standard IP technologies, but the downstream side is always masked with the proprietary protocols used for the wireless link (like ZigBee, Bluetooth, RFID, etc.). This work presents a novel solution (WebTag) for a direct IP based access to a sensor tag over the Near Field Communication (NFC) technology for secure applications. WebTag allows a direct web access to the sensor tag by means of a standard web browser, it reads the sensor data, configures the sampling rate and implements IP based security policies. It is, definitely, a new step towards the evolution of the Internet of Things paradigm. PMID:23012511

Echevarria, Juan Jose; Ruiz-de-Garibay, Jonathan; Legarda, Jon; Alvarez, Maite; Ayerbe, Ana; Vazquez, Juan Ignacio

2012-01-01

154

Quantifying Visual-Representativeness of Social Image Tags Using Image Tag Clarity  

NASA Astrophysics Data System (ADS)

Tags associated with images in various social media sharing web sites are valuable information source for superior image retrieval experiences. Due to the nature of tagging, many tags associated with images are not visually descriptive. In this chapter, we propose Image Tag Clarity to evaluate the effectiveness of a tag in describing the visual content of its annotated images, which is also known as the image tag visual-representativeness. It is measured by computing the zero-mean normalized distance between the tag language model estimated from the images annotated by the tag and the collection language model. The tag/collection language models are derived from the bag of visual-word local content features of the images. The visual-representative tags that are commonly used to annotate visually similar images are given high tag clarity scores. Evaluated on a large real-world dataset containing more than 269K images and their associated tags, we show that the image tag clarity score can effectively identify the visual-representative tags from all tags contributed by users. Based on the tag clarity scores, we have made a few interesting observations that could be used to support many tag-based applications.

Sun, Aixin; Bhowmick, Sourav S.

155

Introduction of a (poly)histidine tag in L-lactate dehydrogenase produces a mixture of active and inactive molecules.  

PubMed

A (poly)histidine tag was fused to either the N- or the C-terminus of L-lactate dehydrogenase (LDH) of Bacillus stearothermophilus to facilitate purification and immobilization of these enzymes. The C-terminally tagged enzyme displayed lower activity compared both to the wild-type and to the N-terminally tagged variant. The reason for this loss of activity was investigated by affinity chromatography of the enzymes on a 5'-AMP-Sepharose resin and by size-exclusion chromatography. The C-terminally tagged enzyme could be separated into an inactive, unbound fraction and an active, bound fraction. Further differences between the C-terminally tagged enzyme and the N-terminally tagged and wild-type LDH were observed on size-exclusion chromatography of the three enzymes. These data suggest that the introduction of a "his-tag" at the C-terminus may induce misfolding of the LDH and serve as a warning that the introduction of a (poly)histidine tag can produce unforseen changes in a protein. PMID:11488630

Halliwell, C M; Morgan, G; Ou, C P; Cass, A E

2001-08-15

156

RNase One Gene Isolation, Expression, and Affinity Purification Models Research Experimental Progression and Culminates with Guided Inquiry-Based Experiments  

ERIC Educational Resources Information Center

This new biochemistry laboratory course moves through a progression of experiments that generates a platform for guided inquiry-based experiments. RNase One gene is isolated from prokaryotic genomic DNA, expressed as a tagged protein, affinity purified, and tested for activity and substrate specificity. Student pairs present detailed explanations…

Bailey, Cheryl P.

2009-01-01

157

Anti-DYKDDDDK G1 Affinity Resin Cat. No. L00432 Technical Manual No. TM0634 Version 03222012  

E-print Network

detection and purification. GenScript Anti-DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK-tagged protein from commonly used protein expression systems including bacteria, yeast at certain concentrations II. Equipments and Reagents Required But Not Supplied Distilled water

Lebendiker, Mario

158

In vitro affinity screening of protein and peptide binders by megavalent bead surface display  

PubMed Central

The advent of protein display systems has provided access to tailor-made protein binders by directed evolution. We introduce a new in vitro display system, bead surface display (BeSD), in which a gene is mounted on a bead via strong non-covalent (streptavidin/biotin) interactions and the corresponding protein is displayed via a covalent thioether bond on the DNA. In contrast to previous monovalent or low-copy bead display systems, multiple copies of the DNA and the protein or peptide of interest are displayed in defined quantities (up to 106 of each), so that flow cytometry can be used to obtain a measure of binding affinity. The utility of the BeSD in directed evolution is validated by library selections of randomized peptide sequences for binding to the anti-hemagglutinin (HA) antibody that proceed with enrichments in excess of 103 and lead to the isolation of high-affinity HA-tags within one round of flow cytometric screening. On-bead Kd measurements suggest that the selected tags have affinities in the low nanomolar range. In contrast to other display systems (such as ribosome, mRNA and phage display) that are limited to affinity panning selections, BeSD possesses the ability to screen and rank binders by their affinity in vitro, a feature that hitherto has been exclusive to in vivo multivalent cell display systems (such as yeast display). PMID:23980186

Diamante, Letizia; Gatti-Lafranconi, Pietro; Schaerli, Yolanda; Hollfelder, Florian

2013-01-01

159

HCI gesture tracking using wearable passive tags  

E-print Network

In this thesis. a wearable system is developed to track hand gestures with passive RFID sensor tags. This system was composed of an ultra-high frequency reader and small, passive, finger-worn tags powered by scavenged RFID ...

Bainbridge, Rachel M

2010-01-01

160

Freedom System Text and Graphics System (TAGS)  

NASA Technical Reports Server (NTRS)

The Text and Graphics System (TAGS) is a high-resolution facsimile system that scans text or graphics material and converts the analog SCAN data into serial digital data. This video shows the TAGS in operation.

1989-01-01

161

Page 1 of 1 Fish Tagging Forum  

E-print Network

and discussion of additional information needs. 10:30 to 11:30 Scenario Analysis ­ Coded Wire Tags (Kevin Kytola:00 Scenario Analysis ­ CWT Continued (Kevin Kytola) Possibly begin discussion of PIT tags if time allows. 3

162

OpenTag: Privacy protection for RFID  

E-print Network

Radio frequency identification's use in retail is good for pervasive computing, but raises considerable privacy issues. OpenTag programmable tags address privacy issues while remaining fully compatible with the supply-chain ...

Holtzman, Henry N.

163

Comparing Social Tags to Microblogs  

Microsoft Academic Search

As Internet usage and e-commerce grow, online social media serve as popular outlets for consumers to express sentiments about products. On Amazon, users can tag an album with a keyword, while tweets on Twitter represent a more natural conversation. The differing natures of these media make them difficult to compare. This project collects and analyzes social media data for newly

Victoria Lai; Christopher Rajashekar; William Rand

2011-01-01

164

What Do Tag Games Teach?  

ERIC Educational Resources Information Center

Tag games have been described as "Chasing, fleeing, and dodging" type activities. Most "fleeing" activities involve dramatic play, use of movement concepts (such as quick and light), or movement changes without a partner, while many of the chasing and dodging activities utilize dodging concepts between partners or within small groups and are…

Belka, David

2006-01-01

165

A Survey of RFID Tags  

Microsoft Academic Search

The Radio Frequency Identification System (RFID) has become a popular system and its applications has reached in most of the fields like toll bridge, supply chain management and defense sector (8). The RFID has also entered into the field of medical sciences (4, 7). Everyday, RFID tags are becoming very small and their dimensions are also reducing to 0.002 inches

M Ayoub Khan; Manoj Sharma; Brahmanandha Prabhu R

2009-01-01

166

Simple chemical syntheses of TAG monohydroperoxides  

Microsoft Academic Search

For the purpose of synthesizing standards to be used in the quantification of TAG hydroperoxides, three TAG (1,2-dioleoyl-3-palmitoylglycerol,\\u000a 1-oleoyl-2-linoleoyl-3-palmitoylglycerol, and triolein) monohydroperoxides were chemically synthesized as authentic specimens.\\u000a TAG were prepared by using a simple condensation in pyridine of glycerol and the corresponding acid chlorides. These TAG were\\u000a then converted into monohydroperoxides by a photosensitized peroxidation. The synthesized monohydroperoxides were

Shu-Ping Hui; Tsuyoshi Murai; Teruki Yoshimura; Hitoshi Chiba; Takao Kurosawa

2003-01-01

167

WhaleNet's Satellite Tagging Program  

NSDL National Science Digital Library

This extensive, easy to use site contains information on satellite tags and tagging, data from current and archived tagging projects on whales, porpoises, seals, and turtles, and questions and activities to help use the data in the classroom. Follow these marine mammals up and down the coast or use the archived data to study distribution and migration patterns of dolphins, seals, etc. Site also links to other tagging and monitoring programs.

168

The electron affinity of phenanthrene.  

PubMed

Phenanthrene is studied by photodetachment-photoelectron spectroscopy. Due to the absence of a parent ion peak in the anion mass spectrum the electron affinity could not be determined directly. However, this absence is the first indication that this molecule has a negative electron affinity. The first three water complexes of phenanthrene were studied, supplying insights into its microsolvation property. Moreover, the electron affinity of the bare molecule could be determined to be -0.01+/-0.04 eV by an extrapolation method using the water cluster data. The experimental work is supported by ab initio calculations for determining the structure of the water complexes. Finally a correlation between the electron affinity and the reduction potential of polycyclic aromatic hydrocarbons is investigated. PMID:17129106

Tschurl, Martin; Boesl, Ulrich; Gilb, Stefan

2006-11-21

169

PPS-Tags: Physical, Perceptual and Semantic Tags for Autonomous Mobile Manipulation  

Microsoft Academic Search

For many promising application areas, au- tonomous mobile manipulators do not yet exhibit sufficiently robust performance. We propose the use of tags applied to task-relevant locations in human environments in order to help autonomous mobile manipulators physically interact with the location, perceive the location, and understand the location's semantics. We call these tags physical, perceptual and semantic tags (PPS-tags). We

Hai Nguyen; Travis Deyle; Matt Reynolds; Charles C. Kemp

170

The Blocker Tag: Selective Blocking of RFID Tags for Consumer Privacy  

E-print Network

The Blocker Tag: Selective Blocking of RFID Tags for Consumer Privacy Ari Juels RSA Laboratories unwanted scanning of RFID tags attached to items they may be carrying or wearing. While an ordinary RFID in supply-chain management, a blocker tag is a cheap passive RFID device that can simulate many ordinary

Rivest, Ronald L.

171

Vaccine Efficacy and Affinity Maturation  

NASA Astrophysics Data System (ADS)

We propose macroscopic equations to describe variable vaccine efficacy between repeated vaccinee and first time vaccinee. The main ingredients are antigenic distance between epidemic strain and vaccne strain, and affinity maturation dynamics which differs in primary and second response. Increase of affinity by repeated vaccine leads to localization in immune space. This localization decreases the ability of the immune system to response to distant, but related epidemic strains.

Lee, Hayoun; Deem, Michael W.

2002-03-01

172

AMERICAN LOBSTERS TAGGED BY MAINE COMMERCIAL FISHERMEN,  

E-print Network

AMERICAN LOBSTERS TAGGED BY MAINE COMMERCIAL FISHERMEN, 1957-59 In 1957 at the suggestion of C. Owen Smith, then editor of the "Maine Coast Fisherman," several commercial lobster fishermen volunteered to tag illegal American lobster, Homarus ameri- canus, with tags furnished by the Maine Depart

173

Efficient Object Identification with Passive RFID Tags  

Microsoft Academic Search

Radio frequency identification systems with passive tags are power- ful tools for object identification. However, if multiple tags are to be identified simultaneously, messages from the tags can collide and cancel each other out. Therefore, multiple read cycles have to be performed in order to achieve a high recognition rate. For a typical stochastic anti-collision scheme, we show how to

Harald Vogt

2002-01-01

174

Requesting Pervasive Services by Touching RFID Tags  

Microsoft Academic Search

We suggest a general framework for requesting pervasive services by touching RFID tags. The tags conne ct the physical and digital environments. Visual symbols c ommunicate to users the objects that can be touched and the services that can be activated. When a user touches such a symbol with a mobile phone, the data stored in the tag and other

Jukka Riekki; Timo Salminen; Ismo Alakärppä

2006-01-01

175

RFID Performance Tag Analysis Dan Deavours,  

E-print Network

RFID Performance Tag Analysis Dan Deavours, Karthik Moncombu Ramakrishnan, and Afzal Syed ITTC This report describes efforts to measure performance of item-level EPC-based passive UHF RFID tags on cell phones. The intended application is to use RFID tags to keep unauthorized cell phones from restricted

Kansas, University of

176

Multiple object identification with passive RFID tags  

Microsoft Academic Search

We investigate the applicability of passive RFID systems to the task of identifying multiple tagged objects simultaneously, assuming that the number of tags is not known in advance. We present a combinatorial model of the communication mechanism between the reader device and the tags, and use this model to derive the optimal parameter setting for the reading process, based on

Harald Vogt

2002-01-01

177

Implanting Telemetry Tag in Pallid Sturgeon  

USGS Multimedia Gallery

Acoustic telemetry tags have to be fairly small to fit inside a five to fifteen pound pallid sturgeon.  The average tag is about the size of your ring finger (60-90 mm long and 16 mm diameter).  That means the battery that powers the acoustic tag is small, too.  A small battery means ...

178

Method for designing gas tag compositions  

DOEpatents

For use in the manufacture of gas tags such as employed in a nuclear reactor gas tagging failure detection system, a method for designing gas tagging compositions utilizes an analytical approach wherein the final composition of a first canister of tag gas as measured by a mass spectrometer is designated as node No. 1. Lattice locations of tag nodes in multi-dimensional space are then used in calculating the compositions of a node No. 2 and each subsequent node so as to maximize the distance of each node from any combination of tag components which might be indistinguishable from another tag composition in a reactor fuel assembly. Alternatively, the measured compositions of tag gas numbers 1 and 2 may be used to fix the locations of nodes 1 and 2, with the locations of nodes 3-N then calculated for optimum tag gas composition. A single sphere defining the lattice locations of the tag nodes may be used to define approximately 20 tag nodes, while concentric spheres can extend the number of tag nodes to several hundred. 5 figures.

Gross, K.C.

1995-04-11

179

Electromagnetic Tagging for Electronic Music Interfaces  

Microsoft Academic Search

This paper describes the development of a musical interface based on electromagnetic tagging technology, where an ensemble of passively tagged objects is identified and tracked in real time when placed in the vicinity of a reader. As the system is able to identify and update the state of many (30 or more) tags simultaneously, they can be used together in

Joseph A. Paradiso; Laurel S. Pardue; Kai-Yuh Hsiao; Ari Y. Benbasat

2003-01-01

180

Fabrication process for a flexible tag microlab  

Microsoft Academic Search

The aim of this paper is to present an integrated process flow for a smart tag with integrated sensors and RFID communication, a Flexible Tag Microlab (FTM). The heart of the designed container tracing system is an RFID system (Reader + Tag) with gas sensing capabilities on board. In the former prototypes, the chemical sensors were integrated on the reader,

E. Abad; B. Mazzolai; A. Juarros; D. Gómez; A. Mondini; I. Sayhan; A. Krenkow; Th. Becker

2007-01-01

181

Enhancement of drug delivery to bone: Characterization of human tissue-nonspecific alkaline phosphatase tagged with an acidic oligopeptide  

PubMed Central

Hypophosphatasia is caused by deficiency of activity of the tissue-nonspecific alkaline phosphatase (TNSALP), resulting in a defect of bone mineralization. Enzyme replacement therapy (ERT) with partially purified plasma enzyme was attempted but with little clinical improvement. Attaining clinical effectiveness with ERT for hypophosphatasia may require delivering functional TNSALP enzyme to bone. We tagged the C-terminal-anchorless TNSALP enzyme with an acidic oligopeptide (a six or eight residue stretch of L-Asp), and compared the biochemical properties of the purified tagged and untagged enzymes derived from Chinese hamster ovary cell lines. The specific activities of the purified enzymes tagged with the acidic oligopeptide were the same as the untagged enzyme. In vitro affinity experiments showed the tagged enzymes had 30-fold higher affinity for hydroxyapatite than the untagged enzyme. Lectin affinity chromatography for carbohydrate structure showed little difference among the three enzymes. Biodistribution pattern from single infusion of the fluorescence-labeled enzymes into mice showed delayed clearance from the plasma up to 18h post infusion and the amount of tagged enzyme retained in bone was 4-fold greater than that of the untagged enzyme. In vitro mineralization assays with the bone marrow from a hypophosphatasia patient using each of the three enzymes in the presence of high concentrations of pyrophosphate provided evidence of bone mineralization. These results show the anchorless enzymes tagged with an acidic oligopeptide are delivered efficiently to bone and function bioactively in bone mineralization, at least in vitro. They suggest potential advantages for use of these tagged enzymes in ERT for hypophosphatasia, which should be explored. PMID:16616566

Nishioka, Tatsuo; Tomatsu, Shunji; Gutierrez, Monica A.; Miyamoto, Ken-ichi; Trandafirescu, Georgeta G.; Lopez, Patricia L.C.; Grubb, Jeffrey H.; Kanai, Rie; Kobayashi, Hironori; Yamaguchi, Seiji; Gottesman, Gary S.; Cahill, Richard; Noguchi, Akihiko; Sly, William S.

2008-01-01

182

Abstract.North Pacific albacore tag-ging data from a tag-release program  

E-print Network

albacore fishing fleets in the North Pacific, the U.S. baitboat, Japan baitboat, troll and longline fleets than 10% per year since the early 1970s. However, a deficit of returns from the troll fleet depressed availability of tagged alba- core to the troll fleet, annual exploita- tion rates were estimated

183

Dual tagging as an approach to isolate endogenous chromatin remodeling complexes from Saccharomyces cerevisiae.  

PubMed

Affinity isolation has been an essential technique for molecular studies of cellular assemblies, such as the switch/sucrose non-fermentable (SWI/SNF) family of ATP-dependent chromatin remodeling complexes. However, even biochemically pure isolates can contain heterogeneous mixtures of complexes and their components. In particular, purification strategies that rely on affinity tags fused to only one component of a complex may be susceptible to this phenomenon. This study demonstrates that fusing purification tags to two different proteins enables the isolation of intact complexes of remodels the structure of chromatin (RSC). A Protein A tag was fused to one of the RSC proteins and a Twin-Strep tag to another protein of the complex. By mass spectrometry, we demonstrate the enrichment of the RSC complexes. The complexes had an apparent Svedberg value of about 20S, as shown by glycerol gradient ultracentrifugation. Additionally, purified complexes were demonstrated to be functional. Electron microscopy and single-particle analyses revealed a conformational rearrangement of RSC upon interaction with acetylated histone H3 peptides. This purification method is useful to purify functionally active, structurally well-defined macromolecular assemblies. PMID:25486077

Lin, Tzong-Yuan; Voronovsky, Andriy; Raabe, Monika; Urlaub, Henning; Sander, Bjoern; Golas, Monika M

2015-03-01

184

Effect of Gen2 Protocol Parameters on RFID Tag Performance  

E-print Network

Effect of Gen2 Protocol Parameters on RFID Tag Performance Pavel V. Nikitin and K. V. S. Rao.rao@intermec.com Abstract-- In this paper, we analyze the effect of Gen2 protocol parameters on RFID tag performance (tag. INTRODUCTION Today, the dependence of UHF RFID tag performance on various tag parameters (chip sensitivity, tag

Hochberg, Michael

185

Adaptive binary splitting for efficient RFID tag anti-collision  

Microsoft Academic Search

Tag collision arbitration for passive RFID tags is a significant issue for fast tag identification. This letter presents a novel tag anti-collision scheme called adaptive binary splitting (ABS). For reducing collisions, ABS assigns distinct timeslots to tags by using information obtained from the last identification process. Our performance evaluation shows that ABS outperforms other tree based tag anti-collision protocols.

Jihoon Myung; Wonjun Lee; J. Srivastava

2006-01-01

186

Direct Dynamic Protein-Affinity Selection Mass-Spectrometry  

PubMed Central

A new methodology is described enabling the affinity screening of potential ligands towards the human estrogen receptor alpha ligand binding domain (ER?-LBD). In-solution incubation is performed of the analyte and the His-tagged ER?-LBD. The bound complex is immobilized on a nickel-loaded protein-affinity selection column, where after the unbound fraction is removed. The immobilized protein–ligand complex is exposed to a decreased pH value and an increased organic modifier concentration releasing the ligand for MS detection, and precipitating the proteins on a filter positioned between the affinity column and the mass spectrometer. The trapping column can be regenerated for reuse at least 70 times. The advantages of the methodology over existing methodologies are the absence of a pre-concentration as well as a chromatographic separation step, resulting in a significantly shorter analysis time compared to previously described procedures, and in addition, allowing the determination of solutes with unfavorable chromatographic properties. The overall analysis time now can be reduced about 250% to approximately 6 min. Replacing the filters after every measurement results in an intra-day standard deviation of 14.8% and an inter-day standard deviation of 21.3%. PMID:20628447

Jonker, Niels; Irth, Hubertus

2010-01-01

187

Genetic engineering of high affinity anti-human colorectal tumour mouse/human chimeric antibody.  

PubMed Central

Two amino acids, tyrosine at position 96 and histidine at position 99 in the variable heavy chain (VH) CDR3 region of a mouse/human chimeric anti-TAG72 antibody cB72.3-1-3 were substituted with phenylalanine and asparagine respectively by site-directed mutagenesis technique. The expression vector mpSV2neo-EP1-Vm1-3-C gamma 1 containing mutant VH region fragments (Vm1-3) as well as the immunoglobulin enhancer (E), promoter (P1) and human genomic C gamma 1 region fragments, was transfected into a heavy-chain loss mutant cell line B72.3Mut(k). Mutant chimeric cB72.3m1-3 antibodies were purified from the transfectant supernates and compared based upon their binding affinity for the TAG72 antigen relative to that of the original cB72.3-1-3 antibody. The data show that a single amino acid substitution of histidine with asparagine at position 99 in VH CDR3 region contributes to four times increase in binding affinity for the TAG72 antigen. This suggests that the residue at position 99 in VH CDR3 region may play some role in antibody/antigen (B72.3/TAG72) interaction. Images Figure 3 Figure 4 Figure 6 PMID:1551684

Xiang, J; Chen, Z

1992-01-01

188

Protein purification with polymeric affinity membranes containing functionalized poly(acid) brushes.  

PubMed

Porous nylon membranes modified with poly(acid) brushes and their derivatives can rapidly purify proteins via ion-exchange and metal-ion affinity interactions. Membranes containing poly(2-(methacryloyloxy)ethyl succinate) (poly(MES)) brushes bind 118 +/- 8 mg of lysozyme per cm(3) of membrane and facilitate purification of lysozyme from chicken egg white. Moreover, functionalization of the poly(MES) brushes with nitrilotriacetate (NTA)-Ni(2+) complexes yields membranes that bind poly(histidine)-tagged (His-tagged) ubiquitin with a capacity of 85 +/- 2 mg of protein per cm(3) of membrane. Most importantly, the membranes modified with poly(MES)-NTA-Ni(2+) allow isolation of His-tagged cellular retinaldehyde-binding protein directly from a cell extract in <10 min, and the protein purity is comparable to that achieved with commercial affinity columns. Therefore, porous nylon membranes containing functionalized poly(MES) brushes are attractive candidates for rapid, high-capacity purification of His-tagged proteins from cell extracts. PMID:20187657

Jain, Parul; Vyas, Mukesh Kumar; Geiger, James H; Baker, Gregory L; Bruening, Merlin L

2010-04-12

189

Protein Purification with Polymeric Affinity Membranes Containing Functionalized Poly(acid) Brushes  

PubMed Central

Porous nylon membranes modified with poly(acid) brushes and their derivatives can rapidly purify proteins via ion-exchange and metal-ion affinity interactions. Membranes containing poly(2-(methacryloyloxy)ethyl succinate) (poly(MES)) brushes bind 118 ± 8 mg of lysozyme per cm3 of membrane and facilitate purification of lysozyme from chicken egg white. Moreover, functionalization of the poly(MES) brushes with nitrilotriacetate (NTA)-Ni2+ complexes yields membranes that bind poly(histidine)-tagged (His-tagged) ubiquitin with a capacity of 85 ± 2 mg of protein per cm3 of membrane. Most importantly, the membranes modified with poly(MES)-NTA-Ni2+ allow isolation of His-tagged cellular retinaldehyde-binding protein directly from a cell extract in less than 10 min, and the protein purity is comparable to that achieved with commercial affinity columns. Thus, porous nylon membranes containing functionalized poly(MES) brushes are attractive candidates for rapid, high-capacity purification of His-tagged proteins from cell extracts. PMID:20187657

Jain, Parul; Vyas, Mukesh Kumar; Geiger, James H.; Baker, Gregory L.; Bruening, Merlin L.

2010-01-01

190

Synthesis of fluorescently tagged polyelectrolytes  

Microsoft Academic Search

Polymers adsorbed from solution onto pigments impart great influence on adhesion, pigment dispersion, rheology, foaming, wetting, and gloss in coatings. In order to study the kinetics of the adsorption–desorption of polyelectrolytes on solid surfaces, polyelectrolytes with polydispersity <1.2, controlled architecture, and tagged with fluorescent groups were synthesized. Narrow polydispersity poly-t-butylmethacrylates (PtBMA) were synthesized by the atom transfer radical polymerization (ATRP)

Glen E Southard; James T. K Woo; John L Massingill

2004-01-01

191

Tetherless Microgrippers With Transponder Tags  

Microsoft Academic Search

We describe the concept of utilizing tetherless mi- crostructured grippers with attached silicon (Si)-based chips for event-based gripping. Grippers were fabricated using photolitho- graphy, and Si chips were bonded to them using a solder-based directed assembly approach. Because we propose the use of these grippers as tags or to attach electronic devices to various sur- faces, we also attached commercial

Kate E. Laflin; Christopher J. Morris; Noy Bassik; Mustapha Jamal; David H. Gracias

2011-01-01

192

Infrared tag and track technique  

DOEpatents

A method of covertly tagging an object for later tracking includes providing a material capable of at least one of being applied to the object and being included in the object, which material includes deuterium; and performing at least one of applying the material to the object and including the material in the object in a manner in which in the appearance of the object is not changed, to the naked eye.

Partin, Judy K. (Idaho Falls, ID); Stone, Mark L. (Idaho Falls, ID); Slater, John (Albuquerque, NM); Davidson, James R. (Idaho Falls, ID)

2007-12-04

193

Phase modulation in RF tag  

DOEpatents

A radio frequency (RF) communication system employs phase-modulated backscatter signals for RF communication from an RF tag to an interrogator. The interrogator transmits a continuous wave interrogation signal to the RF tag, which based on an information code stored in a memory, phase-modulates the interrogation signal to produce a backscatter response signal that is transmitted back to the interrogator. A phase modulator structure in the RF tag may include a switch coupled between an antenna and a quarter-wavelength stub; and a driver coupled between the memory and a control terminal of the switch. The driver is structured to produce a modulating signal corresponding to the information code, the modulating signal alternately opening and closing the switch to respectively decrease and increase the transmission path taken by the interrogation signal and thereby modulate the phase of the response signal. Alternatively, the phase modulator may include a diode coupled between the antenna and driver. The modulating signal from the driver modulates the capacitance of the diode, which modulates the phase of the response signal reflected by the diode and antenna.

Carrender, Curtis Lee; Gilbert, Ronald W.

2007-02-20

194

Tag retention, growth, and survival of red swamp crayfish marked with a visible implant tag  

USGS Publications Warehouse

Eighty juvenile (means: 42.4 mm total length, 1.6 g) red swamp crayfish Procambarus clarkii were implanted with sequentially numbered visible implant tags and held in the laboratory. Tags were injected transversely into the musculature just beneath the exoskeleton of the third abdominal segment from the cephalothorax; tags were visible upon inspection. An additional 20 crayfish were left untagged and served as controls. After 150 d, tag retention was 80% and all tags were readable. No tagged crayfish died during the study, and no differences in total length or weight were detected between tagged and control crayfish. All individuals molted at least three times during the 150-d study, and some individuals molted up to six times, suggesting that most tags would be permanently retained. The readability in the field without specialized equipment makes the visible implant tag ideal for studies of crayfish ecology, management, and culture.

Isely, J.J.; Stockett, P.E.

2001-01-01

195

Chemical binding affinity estimation using MSB  

NASA Astrophysics Data System (ADS)

Binding affinity can be estimated in several ways in the laboratory but there is no viable way to estimate binding affinity in vivo without assumptions on the number of binding sites. Magnetic spectroscopy of nanoparticle Brownian motion, MSB, measures the rotational Brownian motion. The MSB signal is affected by nanoparticle binding affinity so it provides a mechanism to measure the chemical binding affinity. We present a possible mechanism to quantify the binding affinity and test that mechanism using viscous solutions.

Weaver, John B.; Rauwerdink, Adam M.

2011-03-01

196

Radio tag retention and tag-related mortality among adult sockeye salmon  

USGS Publications Warehouse

Tag retention and tag-related mortality are concerns for any tagging study but are rarely estimated. We assessed retention and mortality rates for esophageal radio tag implants in adult sockeye salmon Oncorhynchus nerka. Migrating sockeye salmon captured at the outlet of Lake Clark, Alaska, were implanted with one of four different radio tags (14.5 ?? 43 mm [diameter ?? length], 14.5 ?? 49 mm, 16 ?? 46 mm, and 19 ?? 51 mm). Fish were observed for 15 to 35 d after tagging to determine retention and mortality rates. The overall tag retention rate was high (0.98; 95% confidence interval [CI], 0.92-1.00; minimum, 33 d), with one loss of a 19-mm ?? 51-mm tag. Mortality of tagged sockeye salmon (0.02; 95% CI, 0-0.08) was similar to that of untagged controls (0.03 [0-0.15]). Sockeye salmon with body lengths (mid-eye to tail fork) of 585-649 mm retained tags as large as 19 ?? 51 mm and those with body lengths of 499-628 mm retained tags as small as 14.5 ?? 43 mm for a minimum of 33 d with no increase in mortality. The tags used in this study represent a suite of radio tags that vary in size, operational life, and cost but that are effective in tracking adult anadromous salmon with little tag loss or increase in fish mortality.

Ramstad, K.M.; Woody, C.A.

2003-01-01

197

Directional Radio-Frequency Identification Tag Reader  

NASA Technical Reports Server (NTRS)

A directional radio-frequency identification (RFID) tag reader has been designed to facilitate finding a specific object among many objects in a crowded room. The device could be an adjunct to an electronic inventory system that tracks RFID-tagged objects as they move through reader-equipped doorways. Whereas commercial RFID-tag readers do not measure directions to tagged objects, the device is equipped with a phased-array antenna and a received signal-strength indicator (RSSI) circuit for measuring direction. At the beginning of operation, it is set to address only the RFID tag of interest. It then continuously transmits a signal to interrogate that tag while varying the radiation pattern of the antenna. It identifies the direction to the tag as the radiation pattern direction of peak strength of the signal returned by the tag. An approximate distance to the tag is calculated from the peak signal strength. The direction and distance can be displayed on a screen. A prototype containing a Yagi antenna was found to be capable of detecting a 915.5-MHz tag at a distance of approximately equal to 15 ft (approximately equal to 4.6 m).

Medelius, Pedro J.; Taylor, John D.; Henderson, John J.

2004-01-01

198

The electron affinity of tungsten  

NASA Astrophysics Data System (ADS)

The electron affinity of tungsten has been measured using laser photodetachment threshold spectroscopy in a collinear geometry. The electron affinity was determined to 6583.6(6) cm-1 by observing the onset of the process when W- ions in the 5d^56s^2 6S5/2 ground state are photodetached producing neutral W atoms in the 5d^46s^2 5D0 ground state. The measured value is in agreement with previous measurements and improves the accuracy by almost two orders of magnitude. Further, a photodetachment signal below the ground state photodetachment threshold was found, which indicates the existence of a bound excited state in W-.

Lindahl, A. O.; Andersson, P.; Diehl, C.; Forstner, O.; Klason, P.; Hanstorp, D.

2010-11-01

199

Cysteine-Specific Cu2+ Chelating Tags Used as Paramagnetic Probes in Double Electron Electron Resonance  

PubMed Central

Double electron electron resonance (DEER) is an attractive technique that is utilized for gaining insight into protein structure and dynamics via nanometer-scale distance measurements. The most commonly used paramagnetic tag in these measurements is a nitroxide spin label, R1. Here, we present the application of two types of high-affinity Cu2+ chelating tags, based on the EDTA and cyclen metal-binding motifs as alternative X-band DEER probes, using the B1 immunoglobulin-binding domain of protein G (GB1) as a model system. Both types of tags have been incorporated into a variety of protein secondary structure environments and exhibit high spectral sensitivity. In particular, the cyclen-based tag displays distance distributions with comparable distribution widths and most probable distances within 1–3 Å when compared to homologous R1 distributions. The results display the viability of the cyclen tag as an alternative to the R1 side chain for X-band DEER distance measurements in proteins. PMID:25608028

Cunningham, Timothy F.; Shannon, Matthew D.; Putterman, Miriam R.; Arachchige, Rajith J.; Sengupta, Ishita; Gao, Min; Jaroniec, Christopher P.; Saxena, Sunil

2015-01-01

200

Cysteine-specific cu(2+) chelating tags used as paramagnetic probes in double electron electron resonance.  

PubMed

Double electron electron resonance (DEER) is an attractive technique that is utilized for gaining insight into protein structure and dynamics via nanometer-scale distance measurements. The most commonly used paramagnetic tag in these measurements is a nitroxide spin label, R1. Here, we present the application of two types of high-affinity Cu(2+) chelating tags, based on the EDTA and cyclen metal-binding motifs as alternative X-band DEER probes, using the B1 immunoglobulin-binding domain of protein G (GB1) as a model system. Both types of tags have been incorporated into a variety of protein secondary structure environments and exhibit high spectral sensitivity. In particular, the cyclen-based tag displays distance distributions with comparable distribution widths and most probable distances within 1-3 Å when compared to homologous R1 distributions. The results display the viability of the cyclen tag as an alternative to the R1 side chain for X-band DEER distance measurements in proteins. PMID:25608028

Cunningham, Timothy F; Shannon, Matthew D; Putterman, Miriam R; Arachchige, Rajith J; Sengupta, Ishita; Gao, Min; Jaroniec, Christopher P; Saxena, Sunil

2015-02-19

201

Metal Affinity Resins User Manual  

E-print Network

BD TALONTM Metal Affinity Resins User Manual PT1320-1 (PR34731) Published 29 April 2003 BD Biosciences #12;BD Biosciences Clontech www.bdbiosciences.com Protocol No. PT1320-1 2 Version No. PR34731 BD III. Additional Materials Required 13 IV. Buffers for BD TALONTM Purification & Buffer Kits 17 V

Lebendiker, Mario

202

Affine Contractions on the Plane  

ERIC Educational Resources Information Center

Contractions play a considerable role in the theory of fractals. However, it is not easy to find contractions which are not similitudes. In this study, it is shown by counter examples that an affine transformation of the plane carrying a given triangle onto another triangle may not be a contraction even if it contracts edges, heights or medians.…

Celik, D.; Ozdemir, Y.; Ureyen, M.

2007-01-01

203

The fast affine projection algorithm  

Microsoft Academic Search

This paper discusses a new adaptive filtering algorithm called fast affine projections (FAP). FAP's key features include LMS like complexity and memory requirements (low), and RLS like convergence (fast) for the important case where the excitation signal is speech. Another of FAP's important features is that it causes no delay in the input or output signals. In addition, the algorithm

Steven L. Gay; Sanjeev Tavathia

1995-01-01

204

Securing RFID Systems by Detecting Tag Cloning  

Microsoft Academic Search

Cloning of RFID tags can lead to financil losses in many commercial RFID applications. There are two general strategies to\\u000a provide security: prevention and detection. The security community and the RFID chip manufacturers are currently focused on\\u000a the former by making tags hard to clone. This paper focuses on the latter by investigating a method to pinpoint tags with\\u000a the

Mikko Lehtonen; Daniel Ostojic; Alexander Ilic; Florian Michahelles

2009-01-01

205

The multiple affinities of ?-dystroglycan.  

PubMed

The dystroglycan (DG) adhesion complex is formed by the peripheral ?-DG and the transmembrane ?-DG, both originating from the same precursor. ?-DG plays a crucial role for tissue stability since it binds with high affinity a variety of proteins and proteoglycans in many different cell types. One common molecular feature of most of the ?-DG ligands is the presence of laminin globular (LG) domains that are likely to interact with some of the carbohydrates protruding from the mucin-like region of ?-DG. Every tissue is supposed to produce a specific ?-DG harboring a particular sugar moiety that will enable it to bind a specific ligand, but often several ?-DG ligands are co-expressed within the same tissue. It is therefore very important to assess all these different interactions, ultimately measuring the affinity constants (KDs) underlying them. Herein, we present an updated list of ?-DG interactors, including non LG-domains containing ligands, offering both a historic perspective on the original contributions made by several laboratories and an update on the different techniques used and the KD values obtained so far. For the cure of some muscular dystrophies, the reinstatement of a prominent affinity between ?-DG and one of its vicarious ligands is becoming an increasingly popular choice for strengthening the basement membrane-tissue connection. An update on the current available information about ?- DG's multiple, and often "concomitant" affinities, may be of interest for those wishing to better direct their molecular therapy approaches. A final paragraph is dedicated to comment on the evidence that an increase in affinity is not always advantageous. PMID:24206164

Sciandra, Francesca; Bozzi, Manuela; Bigotti, Maria Giulia; Brancaccio, Andrea

2013-11-01

206

Picture Tags and World Knowledge learning tag rela4ons from visual seman4c sources  

E-print Network

aquarium, hammerhead aquarium, georgia aquarium, fish aquarium, atlanta atlantaons? · Image tagging evalua4ons #12;Which tags are related? aquarium, shark, shark atlanta, georgia atlanta, hammerhead shark, underwater fish, water

Xie, Lexing

207

Heuristic Query Tree Protocol: Use of Known Tags for RFID Tag Anti-Collision  

NASA Astrophysics Data System (ADS)

Existing query tree protocols deal with RFID tags in a blind manner. They query tags in a fixed bit order based on the assumption that the tag ID numbers are uniformly distributed throughout the range of the entire ID space because readers have no prior knowledge of the tags. This paper attempts to distinguish RFID applications where readers are already aware of all tags used by the application. We propose a heuristic query tree (H-QT) protocol that uses heuristic to select effective bits from known tags for the best queries in a divide and conquer approach. The performance evaluation shows that the proposed protocol is superior to original query tree protocols because it significantly reduces the number of tag collisions and no tag response.

Sung, Jongwoo; Kim, Daeyoung; Kim, Taehong; Choi, Jinhyuk

208

SNAP-tag technology: a general introduction.  

PubMed

Over the past few years, the SNAP-tag technology has become a methodology with great potential in a variety of applications, e.g. the (specific) visualization of individual proteins and studies of protein interaction in living cells. Furthermore, the tag can be used for immunopurification and detection of recombinant proteins or site-specific coupling of recombinant proteins to surfaces. Next to the in vitro applications, it also enables detection of tagged proteins in vivo. This review gives an overview of the SNAP-tag technology in different fields of research and its potential for future developments. PMID:23431982

Kolberg, Katharina; Puettmann, Christiane; Pardo, Alessa; Fitting, Jenny; Barth, Stefan

2013-01-01

209

50 CFR 20.81 - Tagging requirement.  

Code of Federal Regulations, 2010 CFR

...IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) MIGRATORY BIRD HUNTING Migratory Bird Preservation Facilities § 20.81 Tagging requirement. No migratory bird preservation facility shall receive or have...

2010-10-01

210

50 CFR 20.81 - Tagging requirement.  

Code of Federal Regulations, 2011 CFR

...IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) MIGRATORY BIRD HUNTING Migratory Bird Preservation Facilities § 20.81 Tagging requirement. No migratory bird preservation facility shall receive or have...

2011-10-01

211

Sensor-based material tagging system  

SciTech Connect

Electronic identification tags are being developed for tracking material and personnel. In applying electronic identification tags to radioactive materials safeguards, it is important to measure attributes of the material to ensure that the tag remains with the material. The addition of a microcontroller with an on-board analog-to-digital converter to an electronic identification tag application-specific integrated-circuit has been demonstrated as means to provide the tag with sensor data. Each tag is assembled into a housing, which serves as a scale for measuring the weight of a paint-can-sized container and its contents. Temperature rise of the can above ambient is also measured, and a piezoelectric detector detects disturbances and immediately puts the tag into its alarm and beacon mode. Radiation measurement was also considered, but the background from nearby containers was found to be excessive. The sensor-based tagging system allows tracking of the material in cans as it is stored in vaults or is moved through the manufacturing process. The paper presents details of the sensor-based material tagging system and describes a demonstration system.

Vercellotti, L.C.; Cox, R.W.; Ravas, R.J.; Schlotterer, J.C. (Westinghouse Electric Corp., Churchill, PA (United States). Science and Technology Center)

1991-01-01

212

Sensor-based material tagging system  

SciTech Connect

Electronic identification tags are being developed for tracking material and personnel. In applying electronic identification tags to radioactive materials safeguards, it is important to measure attributes of the material to ensure that the tag remains with the material. The addition of a microcontroller with an on-board analog-to-digital converter to an electronic identification tag application-specific integrated-circuit has been demonstrated as means to provide the tag with sensor data. Each tag is assembled into a housing, which serves as a scale for measuring the weight of a paint-can-sized container and its contents. Temperature rise of the can above ambient is also measured, and a piezoelectric detector detects disturbances and immediately puts the tag into its alarm and beacon mode. Radiation measurement was also considered, but the background from nearby containers was found to be excessive. The sensor-based tagging system allows tracking of the material in cans as it is stored in vaults or is moved through the manufacturing process. The paper presents details of the sensor-based material tagging system and describes a demonstration system.

Vercellotti, L.C.; Cox, R.W.; Ravas, R.J.; Schlotterer, J.C. [Westinghouse Electric Corp., Churchill, PA (United States). Science and Technology Center

1991-12-31

213

Affinity purification of ribosomes to access the translatome.  

PubMed

We describe ribosome affinity purification (RAP), a method that allows rapid purification of ribosomes and associated messages from the yeast Saccharomyces cerevisiae. The method relies on the expression of protein A tagged versions of the ribosomal protein Rpl16, which is used to efficiently recover endogenously formed ribosomes and polysomes from cellular extracts with IgG-coupled spherical microbeads. This approach can be applied to profile reactions of the translatome, which refers to all messages associated with ribosomes, with those of the transcriptome using DNA microarrays. In addition, ribosomal proteins, their modifications, and/or other associated proteins can be mapped with mass spectrometry. Finally, application of this method in other organisms provides a valuable tool to decipher cell-type specific gene expression patterns. PMID:19398006

Halbeisen, Regula E; Scherrer, Tanja; Gerber, André P

2009-07-01

214

PL-Tags: Detecting Batteryless Tags through the Power Lines in a Building  

Microsoft Academic Search

We present a system, called PL-Tags, for detecting the presence of batteryless tags in a building or home through the power\\u000a lines. The excitation (or interrogation) and detection of these tags occurs wirelessly entirely using the powerline infrastructure\\u000a in a building. The PL-Tags proof-of-concept consists of a single plug-in module that monitors the power line for the presence\\u000a of these

Shwetak N. Patel; Erich P. Stuntebeck; Thomas Robertson

2009-01-01

215

Patterns and Inconsistencies in Collaborative Tagging Systems: An Examination of Tagging Practices  

Microsoft Academic Search

This paper analyzes the tagging patterns exhibited by users of del.icio.us, to assess how collaborative tagging supports and enhances traditional ways of classifying and indexing documents. Using frequency data and co-word analysis matrices analyzed by multi-dimensional scaling, the authors discovered that tagging practices to some extent work in ways that are continuous with conventional indexing. Small numbers of tags tend

Margaret E. I. Kipp; D. Grant Campbell

2006-01-01

216

Biofabrication of ZnS:Mn luminescent nanocrystals using histidine, hexahistidine, and His-tagged proteins: a comparison study.  

PubMed

The ubiquitous hexahistidine purification tag has been used to conjugate proteins to the shell of CdSe:ZnS quantum dots (QDs) due to its affinity for surface-exposed Zn(2+) ions but little attention has been paid to the potential of His-tagged proteins for mineralizing luminescent ZnS nanocrystals. Here, we compare the ability of free histidine, a His tag peptide, His-tagged thioredoxin (TrxA, a monomeric protein), and N- and C-terminally His-tagged versions of Hsp31 (a homodimeric protein) to support the synthesis of Mn-doped ZnS nanocrystals from aqueous precursors under mild conditions of pH (8.2) and temperature (37°C). We find that: (1) it is possible to produce poor quality QDs when histidine is used at high (8 mM) concentration; (2) an increase in local histidine concentration through repetition of the amino acid as a His tag decreases the amount of needed reagent ?10-fold and improves optical properties; (3) fusion of the same His tag to TrxA allows for ZnS:Mn QDs mineralization at micromolar concentrations; and (4) doubling the local hexahistidine concentration by exploiting Hsp31 dimerization further improves nanocrystal luminescence with the brightest particles obtained when His tags are spatially co-localized at the Hsp31 N-termini. Although hexahistidine tracts are not as efficient as combinatorially selected ZnS binding peptides at QD synthesis, it should be possible to use the large number of available His-tagged proteins and the synthesis approach described herein to produce luminescent nanoparticles whose protein shell carries a broad range of functions. PMID:25013361

Zhou, Weibin; Baneyx, François

2014-08-15

217

An Analysis of the Effectiveness of Tagging in Blogs  

Microsoft Academic Search

Tags have recently become popular as a means of annotat- ing and organizing Web pages and blog entries. Advocates of tagging argue that the use of tags produces a 'folksonomy', a system in which the meaning of a tag is determined by its use among the community as a whole. We analyze the effec- tiveness of tags for classifying blog

Christopher H. Brooks; Nancy Montanez

218

Evaluation of visible implant elastomer tags in zebrafish (Danio rerio)  

PubMed Central

Summary The use of the visible implant elastomer (VIE) tagging system in zebrafish (Danio rerio) was examined. Two tag orientations (horizontal and vertical) at the dorsal fin base were tested for tag retention, tag fragmentation and whether VIE tags affected growth and survival of juvenile zebrafish (1–4 month post hatch). Six tag locations (abdomen, anal fin base, caudal peduncle, dorsal fin base, pectoral fin base, isthmus) and 5 tag colors (yellow, red, pink, orange, blue) were evaluated for ease of VIE tag application and tag visibility in adult zebrafish. Long-term retention (1 year) and multiple tagging sites (right and left of dorsal fin and pectoral fin base) were examined in adult zebrafish. Lastly, survival of recombination activation gene 1?/? (rag1?/?) zebrafish was evaluated after VIE tagging. The best tag location was the dorsal fin base, and the most visible tag color was pink. Growth rate of juvenile zebrafish was not affected by VIE tagging. Horizontal tagging is recommended in early stages of fish growth (1–2 months post hatch). VIE tags were retained for 1 year and tagging did not interfere with long-term growth and survival. There was no mortality associated with VIE tagging in rag1?/? zebrafish. The VIE tagging system is highly suitable for small-sized zebrafish. When familiar with the procedure, 120 adult zebrafish can be tagged in one hour. It does not increase mortality in adult zebrafish or interfere with growth in juvenile or adult zebrafish. PMID:24285706

Hohn, Claudia; Petrie-Hanson, Lora

2013-01-01

219

Between 'Instructions' and 'Diy': Tagging in Learning Communities  

Microsoft Academic Search

In this paper, we discuss the novel technology tagging and the results of analyzing a learning commu- nity in a popular system that relies on tagging namely YouTube. We present our findings that young people use tagging as social technology and tags to create a vocabulary among friends and communi- ties. We argue that tags can be used to facilitate

Milena Reichel; Heidi Schelhowe

2008-01-01

220

Theoretical proton affinity and fluoride affinity of nerve agent VX.  

PubMed

Proton affinity and fluoride affinity of nerve agent VX at all of its possible sites were calculated at the RI-MP2/cc-pVTZ//B3LYP/6-31G* and RI-MP2/aug-cc-pVTZ//B3LYP/6-31+G* levels, respectively. The protonation leads to various unique structures, with H(+) attached to oxygen, nitrogen, and sulfur atoms; among which the nitrogen site possesses the highest proton affinity of -?E ? 251 kcal/mol, suggesting that this is likely to be the major product. In addition some H(2), CH(4) dissociation as well as destruction channels have been found, among which the CH(4) + [Et-O-P(?O)(Me)-S-(CH(2))(2)-N(+)(iPr)?CHMe] product and the destruction product forming Et-O-P(?O)(Me)-SMe + CH(2)?N(+)(iPr)(2) are only 9 kcal/mol less stable than the most stable N-protonated product. For fluoridization, the S-P destruction channel to give Et-O-P(?O)(Me)(F) + [S-(CH(2))(2)-N-(iPr)(2)](-) is energetically the most favorable, with a fluoride affinity of -?E ? 44 kcal. Various F(-) ion-molecule complexes are also found, with the one having F(-) interacting with two hydrogen atoms in different alkyl groups to be only 9 kcal/mol higher than the above destruction product. These results suggest VX behaves quite differently from surrogate systems. PMID:21117653

Bera, Narayan C; Maeda, Satoshi; Morokuma, Keiji; Viggiano, Al A

2010-12-23

221

Evacuation of Passive Integrated Transponder (PIT) Tags from Northern Pikeminnow Consuming Tagged Juvenile Chinook Salmon  

Microsoft Academic Search

Prey fish implanted with passive integrated transponder (PIT) tags can be used in predation studies if the timing of tag evacuation from the predators is understood. Laboratory experiments were conducted to determine how PIT tags in juvenile Chinook salmon Oncorhynchus tshawytscha that were consumed by northern pikeminnow Ptychocheilus oregonensis were evacuated in relation to various parameters. The rate of evacuation

James H. Petersen; Craig A. Barfoot

2003-01-01

222

Fish Tagging Forum Draft Compilation of Tagging Data Collection and Management  

E-print Network

of tag/mark related data, management of data using database tools, and the regional accessibilityFish Tagging Forum Draft Compilation of Tagging Data Collection and Management 2012_11_12 v0 Data Management is one topic specified in our charter that we have heard a few things about as part

223

Affinity purification of binding miRNAs for messenger RNA fused with a common tag.  

PubMed

Prediction of microRNA-mRNA interaction typically relies on bioinformatic methods, but these methods only suggest the possibility of microRNA binding and may miss important interactions as well as falsely predict others. A major obstacle to the miRNA research has been the lack of experimental procedures for the identification of miRNA-mRNA interactions. Recently, a few studies have attempted to explore experimental methods to isolate and identify miRNA targets or miRNAs targeting a single gene. Here, we developed an more convenient experimental approach for the isolation and identification of miRNAs targeting a single gene by applying short biotinylated DNA anti-sense oligonucleotides mix to enhanced green fluorescent protein (EGFP) mRNA which was fused to target gene mRNA. This method does not require a design of different anti-sense oligonucleotides to any mRNA. This is a simple and an efficient method to potentially identify miRNAs targeting specific gene mRNA combined with chip screen. PMID:25153630

Wei, Ke; Yan, Feng; Xiao, Hui; Yang, Xiaoxu; Xie, Guie; Xiao, Ye; Wang, Tingting; Xun, Yu; Huang, Zhaoqin; Han, Mei; Zhang, Jian; Xiang, Shuanglin

2014-01-01

224

Enrichment of Phosphorylated Proteins from Cell Lysate -Phosphate Affinity Chromatography using Phos-tagTM Agarose -  

E-print Network

and phosphorylated peptides from biological samples at physiological pH. The phosphate enrichment procedure needs # distilled water 70 mL # 3.0 mol/L aqueous CH3COOH for pH adjustment at 7.5 a proper quantity # distilled # distilled water 80 mL # 3.0 mol/L aqueous CH3COOH for pH adjustment at 7.5 a proper quantity # distilled

Lebendiker, Mario

225

To tag or not to tag: animal welfare, conservation and stakeholder considerations in fish tracking studies that use electronic tags  

SciTech Connect

The advent and widespread adoption of electronic tags (including biotelemetry and biologging devices) for tracking animals has provided unprecedented information on the biology, management, and conservation of fish in the world’s oceans and inland waters. However, use of these tools is not without controversy. Even when scientific and management objectives may best be achieved using electronic tags, it is increasingly important to further consider other factors such as the welfare of tagged animals (i.e., the role of training and science-based surgical guidelines, anesthetic use, inability to maintain sterile conditions in field environments), the ethics of tagging threatened species vs. using surrogates, stakeholder perspectives on tagging (including aboriginals), as well as use of data emanating from such studies (e.g., by fishers to facilitate exploitation). Failure to do so will have the potential to create conflict and undermine scientific, management and public confidence in the use of this powerful tool. Indeed, there are already a number of examples of where tracking studies using electronic tags have been halted based on concerns raised by researchers, authorities, or stakeholders. Here we present a candid evaluation of several factors that should be considered when determining when to tag or not to tag fish with electronic devices. It is not our objective to judge the merit of previous studies. Rather, we hope to stimulate debate and discussion regarding the use of electronic tags to study fish. Relatedly, there is a need for more research to address these questions (e.g., what level of cleanliness is needed when conducting surgeries, what type of training should be required for fish surgery) including human dimensions studies to understand perspectives of different actors including society as a whole with respect to tagging and tracking studies.

Cooke, Steven J.; Nguyen, Vivian M.; Murchie, Karen J.; Thiem, Jason D.; Donaldson, Michael R.; Hinch, Scott G.; Brown, Richard S.; Fisk, Aaron

2013-11-01

226

50 CFR 20.36 - Tagging requirement.  

Code of Federal Regulations, 2010 CFR

...WILDLIFE AND PLANTS (CONTINUED) MIGRATORY BIRD HUNTING Possession § 20.36 Tagging...person shall put or leave any migratory game birds at any place (other than at his personal...taxidermy services performed, unless such birds have a tag attached, signed by the...

2010-10-01

227

50 CFR 20.36 - Tagging requirement.  

Code of Federal Regulations, 2011 CFR

...WILDLIFE AND PLANTS (CONTINUED) MIGRATORY BIRD HUNTING Possession § 20.36 Tagging...person shall put or leave any migratory game birds at any place (other than at his personal...taxidermy services performed, unless such birds have a tag attached, signed by the...

2011-10-01

228

50 CFR 20.36 - Tagging requirement.  

Code of Federal Regulations, 2012 CFR

...WILDLIFE AND PLANTS (CONTINUED) MIGRATORY BIRD HUNTING Possession § 20.36 Tagging...person shall put or leave any migratory game birds at any place (other than at his personal...taxidermy services performed, unless such birds have a tag attached, signed by the...

2012-10-01

229

USE OF DYNAMITE TO RECOVER TAGGED SALMON  

E-print Network

353 USE OF DYNAMITE TO RECOVER TAGGED SALMON Marine Biological Laboratory LIBRARY Of. zi 1960 WOODS of Commercial Fisheries, Donald L. McKernan, Director USE OF DYNAMITE TO RECOVER TAGGED SALMON by Richard W Page The effect of dynamite on salmon 2 Description and results of variables tested 3 Effect of water

230

A Radio Tag for Big Whales  

ERIC Educational Resources Information Center

Radio tags to track wildlife have been used for years. However, such tagging of whales has been more complicated and less successful. This article explores the latest technology that is designed to give information over a long period of time. (MA)

Watkins, William A.

1978-01-01

231

MFR PAPER 1070 Anchor tags show ment  

E-print Network

a high retention rate. be nonlethal. and quicl-. to apply. The recently developed Floy anchor taggi ng spines. The T bar po rtion of the tag was insert ed through the carapace into the anterior portion of the right branchial cavity . The anchor po rti on of th e tag often failed to return to its norm al shape

232

Harnessing Collective Knowledge Inherent in Tag Clouds  

ERIC Educational Resources Information Center

Tagging systems represent the conceptual knowledge of a community. We experimentally tested whether people harness this collective knowledge when navigating through the Web. As a within-factor we manipulated people's prior knowledge (no knowledge vs. prior knowledge that was congruent/incongruent to the collective knowledge inherent in the tags).…

Cress, U.; Held, C.

2013-01-01

233

Environmental effects on RFID tag antennas  

Microsoft Academic Search

We have studied the effects of nearby objects on the read range of several types of RFID tags, and the impedance, pattern, and radiative efficiency of antennas that closely emulate the tag structures, using measurements and simulations. We find that the main reason for the decrease in read range at perpendicular incidence in close proximity to metals or dielectrics (such

Daniel M. Dobkin; Steven M. Weigand

2005-01-01

234

Tagging and Morphological Disambiguation of Turkish Text  

Microsoft Academic Search

Automatic text tagging is an important component in higher level analysis of text corpora, and its output can be used in many natural language processing applica- tions. In languages like Turkish or Finnish, with agglutinative morphology, morpholog- ical disambiguation is a very crucial pro- cess in tagging, as the structures of many lexical forms are morphologically ambigu- ous. This paper

Kemal Oflazer; Ilker Kuruoz

1994-01-01

235

Tagging English Text with a Probabilistic Model  

Microsoft Academic Search

In this paper we present some experiments on the use of a probabilistic model to tag English text, i.e. to assign to each word the correct tag (part of speech) in the context of the sentence. The main novelty of these experiments is the use of untagged text in the training of the model. We have used a simple triclass

Bernard Merialdo

1994-01-01

236

TAG (Teaching Active Games) for the Holidays  

ERIC Educational Resources Information Center

Holidays present the perfect opportunity for physical educators to utilize creative TAG (Teaching Active Games) games to offer maximum physical activity opportunities for their students. The TAG ideas in this article offer physical education teachers quick, instant activities that involve very little equipment, time management, or instruction. At…

Erwin, Heather E.; Bachtel, Amy

2007-01-01

237

Towards the Semantic Web: Collaborative Tag Suggestions  

Microsoft Academic Search

Content organization over the Internet went through several interesting phases of evolution: from structured di rectories to unstructured Web search engines and more recently, to tagging as a way for aggregating information, a step toward s the semantic web vision. Tagging allows ranking and dat a organization to directly utilize inputs from end us ers, enabling machine processing of Web

Zhichen Xu; Yun Fu; Jianchang Mao; Difu Su

2006-01-01

238

Flavour tag studies with the LCFIVertex package  

E-print Network

In this contribution the status of the flavour tagging performance for the LDCPrime detector model and studies of the track selection parameters and effects from beam backgrounds in flavour tagging using the LCFIVertex package are presented. This work is part of an effort towards a default configuration for the ILD detector optimisation.

Roberval Walsh

2009-01-30

239

Method and apparatus for manufacturing gas tags  

DOEpatents

For use in the manufacture of gas tags employed in a gas tagging failure detection system for a nuclear reactor, a plurality of commercial feed gases each having a respective noble gas isotopic composition are blended under computer control to provide various tag gas mixtures having selected isotopic ratios which are optimized for specified defined conditions such as cost. Using a new approach employing a discrete variable structure rather than the known continuous-variable optimization problem, the computer controlled gas tag manufacturing process employs an analytical formalism from condensed matter physics known as stochastic relaxation, which is a special case of simulated annealing, for input feed gas selection. For a tag blending process involving M tag isotopes with N distinct feed gas mixtures commercially available from an enriched gas supplier, the manufacturing process calculates the cost difference between multiple combinations and specifies gas mixtures which approach the optimum defined conditions. The manufacturing process is then used to control tag blending apparatus incorporating tag gas canisters connected by stainless-steel tubing with computer controlled valves, with the canisters automatically filled with metered quantities of the required feed gases.

Gross, Kenny C. (Bolingbrook, IL); Laug, Matthew T. (Idaho Falls, ID)

1996-01-01

240

Method and apparatus for manufacturing gas tags  

DOEpatents

For use in the manufacture of gas tags employed in a gas tagging failure detection system for a nuclear reactor, a plurality of commercial feed gases each having a respective noble gas isotopic composition are blended under computer control to provide various tag gas mixtures having selected isotopic ratios which are optimized for specified defined conditions such as cost. Using a new approach employing a discrete variable structure rather than the known continuous-variable optimization problem, the computer controlled gas tag manufacturing process employs an analytical formalism from condensed matter physics known as stochastic relaxation, which is a special case of simulated annealing, for input feed gas selection. For a tag blending process involving M tag isotopes with N distinct feed gas mixtures commercially available from an enriched gas supplier, the manufacturing process calculates the cost difference between multiple combinations and specifies gas mixtures which approach the optimum defined conditions. The manufacturing process is then used to control tag blending apparatus incorporating tag gas canisters connected by stainless-steel tubing with computer controlled valves, with the canisters automatically filled with metered quantities of the required feed gases. 4 figs.

Gross, K.C.; Laug, M.T.

1996-12-17

241

Multiyear tagging studies incorporating fishing effort data  

E-print Network

, William S. Hearn, and Kenneth H. Pollock Abstract: The Brownie models for multiyear tagging studies can animals that are secretly added to the fishers' catches. Résumé : Les modèles de Brownie utilisés pour des] Introduction Brownie et al. (1978, 1985) developed a series of models for multiyear tagging studies that allow

Newman, Michael C.

242

One-step purification and structural characterization of a recombinant His-tag 11S globulin expressed in transgenic tobacco.  

PubMed

Amarantin, an 11S globulin, is one of the most important storage proteins of amaranth seeds, with relevant nutritional-functional and nutraceutical characteristics. Its cDNA was cloned in-frame with a sequence encoding a polyhistidine tag and expressed under the direction of a 35S promoter in transgenic tobacco seeds. The presence of a (His)(6) tag on the polypeptide permitted a high-yield single-step purification using immobilized metal-ion affinity chromatography and rapid characterization. Purified His-tag amarantin accounted for up to 5% of total soluble seed protein. Biochemical characterization indicated that purified His-tag amarantin migrated with the expected molecular weight (53 kDa) and was correctly processed into an acidic polypeptide (32 kDa) with isoelectric point (pI) of 5.58 and a basic polypeptide (21 kDa) with pI of 9.24, linked by a disulfide bridge. Moreover, His-tag amarantin was assembled into both homo- and hetero-hexameric 11S structures. These results show that the His tag did not change the biochemical and physicochemical properties of amarantin. The strategy presented here for rapid and high-yield expression and purification procedure should facilitate structure-function studies for this nutritional protein. PMID:15639103

Valdez-Ortiz, Angel; Rascón-Cruz, Quintín; Medina-Godoy, Sergio; Sinagawa-García, Sugey R; Valverde-González, María E; Paredes-López, Octavio

2005-02-23

243

Efficient and versatile one-step affinity purification of in vivo biotinylated proteins: Expression, characterization and structure analysis of recombinant human glutamate carboxypeptidase II  

SciTech Connect

Affinity purification is a useful approach for purification of recombinant proteins. Eukaryotic expression systems have become more frequently used at the expense of prokaryotic systems since they afford recombinant eukaryotic proteins with post-translational modifications similar or identical to the native ones. Here, we present a one-step affinity purification set-up suitable for the purification of secreted proteins. The set-up is based on the interaction between biotin and mutated streptavidin. Drosophila Schneider 2 cells are chosen as the expression host, and a biotin acceptor peptide is used as an affinity tag. This tag is biotinylated by Escherichia coli biotin-protein ligase in vivo. We determined that localization of the ligase within the ER led to the most effective in vivo biotinylation of the secreted proteins. We optimized a protocol for large-scale expression and purification of AviTEV-tagged recombinant human glutamate carboxypeptidase II (Avi-GCPII) with milligram yields per liter of culture. We also determined the 3D structure of Avi-GCPII by X-ray crystallography and compared the enzymatic characteristics of the protein to those of its non-tagged variant. These experiments confirmed that AviTEV tag does not affect the biophysical properties of its fused partner. Purification approach, developed here, provides not only a sufficient amount of highly homogenous protein but also specifically and effectively biotinylates a target protein and thus enables its subsequent visualization or immobilization.

Tykvart, J.; Sacha, P.; Barinka, C.; Knedlik, T.; Starkova, J.; Lubkowski, J.; Konvalinka, J. (Gilead); (NCI); (Czech Academy)

2012-02-07

244

29 CFR 1926.200 - Accident prevention signs and tags.  

Code of Federal Regulations, 2010 CFR

... 2010-07-01 false Accident prevention signs and tags. 1926.200 Section...Barricades § 1926.200 Accident prevention signs and tags. (a) General...locations.html. (h) Accident prevention tags. (1) Accident...

2010-07-01

245

Innate immunity probed by lipopolysaccharides affinity strategy and proteomics.  

PubMed

Lipopolysaccharides (LPSs) are ubiquitous and vital components of the cell surface of Gram-negative bacteria that have been shown to play a relevant role in the induction of the immune-system response. In animal and plant cells, innate immune defenses toward microorganisms are triggered by the perception of pathogen associated molecular patterns. These are conserved and generally indispensable microbial structures such as LPSs that are fundamental in the Gram-negative immunity recognition. This paper reports the development of an integrated strategy based on lipopolysaccharide affinity methodology that represents a new starting point to elucidate the molecular mechanisms elicited by bacterial LPS and involved in the different steps of innate immunity response. Biotin-tagged LPS was immobilized on streptavidin column and used as a bait in an affinity capture procedure to identify protein partners from human serum specifically interacting with this effector. The complex proteins/lipopolysaccharide was isolated and the protein partners were fractionated by gel electrophoresis and identified by mass spectrometry. This procedure proved to be very effective in specifically binding proteins functionally correlated with the biological role of LPS. Proteins specifically bound to LPS essentially gathered within two functional groups, regulation of the complement system (factor H, C4b, C4BP, and alpha 2 macroglobulin) and inhibition of LPS-induced inflammation (HRG and Apolipoproteins). The reported strategy might have important applications in the elucidation of biological mechanisms involved in the LPSs-mediated molecular recognition and anti-infection responses. PMID:22752448

Giangrande, Chiara; Colarusso, Lucia; Lanzetta, Rosa; Molinaro, Antonio; Pucci, Piero; Amoresano, Angela

2013-01-01

246

Expression and affinity purification of recombinant proteins from plants  

NASA Technical Reports Server (NTRS)

With recent advances in plant biotechnology, transgenic plants have been targeted as an inexpensive means for the mass production of proteins for biopharmaceutical and industrial uses. However, the current plant purification techniques lack a generally applicable, economic, large-scale strategy. In this study, we demonstrate the purification of a model protein, beta-glucuronidase (GUS), by employing the protein calmodulin (CaM) as an affinity tag. In the proposed system, CaM is fused to GUS. In the presence of calcium, the calmodulin fusion protein binds specifically to a phenothiazine-modified surface of an affinity column. When calcium is removed with a complexing agent, e.g., EDTA, calmodulin undergoes a conformational change allowing the dissociation of the calmodulin-phenothiazine complex and, therefore, permitting the elution of the GUS-CaM fusion protein. The advantages of this approach are the fast, efficient, and economical isolation of the target protein under mild elution conditions, thus preserving the activity of the target protein. Two types of transformation methods were used in this study, namely, the Agrobacterium-mediated system and the viral-vector-mediated transformation system. Copyright 2002 Elsevier Science (USA).

Desai, Urvee A.; Sur, Gargi; Daunert, Sylvia; Babbitt, Ruth; Li, Qingshun

2002-01-01

247

Engineering the ATLAS TAG Browser  

NASA Astrophysics Data System (ADS)

ELSSI is a web-based event metadata (TAG) browser and event-level selection service for ATLAS. In this paper, we describe some of the challenges encountered in the process of developing ELSSI, and the software engineering strategies adopted to address those challenges. Approaches to management of access to data, browsing, data rendering, query building, query validation, execution, connection management, and communication with auxiliary services are discussed. We also describe strategies for dealing with data that may vary over time, such as run-dependent trigger decision decoding. Along with examples, we illustrate how programming techniques in multiple languages (PHP, JAVASCRIPT, XML, AJAX, and PL/SQL) have been blended to achieve the required results. Finally, we evaluate features of the ELSSI service in terms of functionality, scalability, and performance.

Zhang, Qizhi; ATLAS Collaboration

2011-12-01

248

Transposon tagging in diploid strawberry.  

PubMed

Fragaria vesca was transformed with a transposon tagging construct harbouring amino terminally deleted maize transposase and EGFP (Ac element), NPTII, CaMV 35S promoter (P35S) driving transposase and mannopine synthase promoter (Pmas) driving EGFP (Ds element). Of 180 primary transgenics, 48 were potential launch pads, 72 were multiple insertions or chimaeras, and 60 exhibited somatic transposition. T? progeny of 32 putative launch pads were screened by multiplex PCR for transposition. Evidence of germ-line transposition occurred in 13 putative launch pads; however, the transposition frequency was too low in three for efficient recovery of transposants. The transposition frequency in the remaining launch pads ranged from 16% to 40%. After self-pollination of the T? launch pads, putative transposants in the T? generation were identified by multiplex PCR. Sequencing of hiTAIL-PCR products derived from nested primers within the Ds end sequences (either P35S at the left border or the inverted repeat at the right border) of T? plants revealed transposition of the Ds element to distant sites in the strawberry genome. From more than 2400 T? plants screened, 103 unique transposants have been identified, among which 17 were somatic transpositions observed in the T? generation. Ds insertion sites were dispersed among various gene elements [exons (15%), introns (23%), promoters (30%), 3' UTRs (17%) as well as intergenically (15%)]. Three-primer (one on either side of the Ds insertion and one within the Ds T-DNA) PCR could be used to identify homozygous T? transposon-tagged plants. The mutant collection has been catalogued in an on-line database. PMID:22845757

Veilleux, Richard E; Mills, Kerri P; Baxter, Aaron J; Upham, Kendall T; Ferguson, Tammy J; Holt, Sarah Hudson; Lu, Nan; Ruiz-Rojas, Juan J; Pantazis, Christopher J; Davis, Cherish M; Lindsay, Robert C; Powell, Frankie L; Dan, Yinghui; Dickerman, Allan W; Oosumi, Teruko; Shulaev, Vladimir

2012-10-01

249

Communication methods, systems, apparatus, and devices involving RF tag registration  

DOEpatents

One technique of the present invention includes a number of Radio Frequency (RF) tags that each have a different identifier. Information is broadcast to the tags from an RF tag interrogator. This information corresponds to a maximum quantity of tag response time slots that are available. This maximum quantity may be less than the total number of tags. The tags each select one of the time slots as a function of the information and a random number provided by each respective tag. The different identifiers are transmitted to the interrogator from at least a subset of the RF tags.

Burghard, Brion J. (W. Richland, WA); Skorpik, James R. (Kennewick, WA)

2008-04-22

250

A fluorogenic probe for SNAP-tagged plasma membrane proteins based on the solvatochromic molecule Nile Red.  

PubMed

A fluorogenic probe for plasma membrane proteins based on the dye Nile Red and SNAP-tag is introduced. It takes advantage of Nile Red, a solvatochromic molecule highly fluorescent in an apolar environment, such as cellular membranes, but almost dark in a polar aqueous environment. The probe possesses a tuned affinity for membranes allowing its Nile Red moiety to insert into the lipid bilayer of the plasma membrane, becoming fluorescent, only after its conjugation to a SNAP-tagged plasma membrane protein. The fluorogenic character of the probe was demonstrated for different SNAP-tag fusion proteins, including the human insulin receptor. This work introduces a new approach for generating a powerful turn-on probe for "no-wash" labeling of plasma membrane proteins with numerous applications in bioimaging. PMID:24471525

Prifti, Efthymia; Reymond, Luc; Umebayashi, Miwa; Hovius, Ruud; Riezman, Howard; Johnsson, Kai

2014-03-21

251

Do-it-yourself histidine-tagged bovine enterokinase: A handy member of the protein engineer's toolbox?  

PubMed Central

Enterokinase, a two-chain duodenal serine protease, activates trypsinogen by removing its N-terminal propeptide. Due to a clean cut after the non-primed site recognition sequence, the enterokinase light chain is frequently employed in biotechnology to separate N-terminal affinity tags from target proteins with authentic N-termini. In order to obtain large quantities of this protease, we adapted an in vitro folding protocol for a pentahistidine-tagged triple mutant of the bovine enterokinase light chain. The purified, highly active enzyme successfully processed recombinant target proteins, while the pentahistidine-tag facilitated post-cleavage removal. Hence, we conclude that producing enterokinase in one's own laboratory is an efficient alternative to the commercial enzyme. PMID:24184090

Skala, Wolfgang; Goettig, Peter; Brandstetter, Hans

2013-01-01

252

Do-it-yourself histidine-tagged bovine enterokinase: a handy member of the protein engineer's toolbox.  

PubMed

Enterokinase, a two-chain duodenal serine protease, activates trypsinogen by removing its N-terminal propeptide. Due to a clean cut after the non-primed site recognition sequence, the enterokinase light chain is frequently employed in biotechnology to separate N-terminal affinity tags from target proteins with authentic N-termini. In order to obtain large quantities of this protease, we adapted an in vitro folding protocol for a pentahistidine-tagged triple mutant of the bovine enterokinase light chain. The purified, highly active enzyme successfully processed recombinant target proteins, while the pentahistidine-tag facilitated post-cleavage removal. Hence, we conclude that producing enterokinase in one's own laboratory is an efficient alternative to the commercial enzyme. PMID:24184090

Skala, Wolfgang; Goettig, Peter; Brandstetter, Hans

2013-12-01

253

Influence of material properties upon immobilization of histidine-tagged protein on Ni-Co coated chip.  

PubMed

In protein research, protein microarray facilitates high-throughput study of protein abundance and function. An appropriate microarray surface that can be used to immobilize protein samples is a prerequisite for the investigation of molecular interactions. Ni-Co alloy coated protein microarray chip has been found to adsorb histidine-tagged proteins effectively based on the method of immobilized metal affinity chromatography. Due to the ingredient of bi-metallic elements, different electroplating conditions resulted in distinct binding affinities. Therefore, the influence of Ni-Co material properties on the immobilization of histidine-tagged protein was systematically investigated in this study. In the experiments, the contact angle measurement suggested that no strong relationship can be established between the wettability of chip surface and its corresponding protein immobilization. ESCA test demonstrated that the major ingredients of the Ni-Co alloy coated protein microarray chip were Ni and Co. In addition, the XRD test concluded that a Ni-Co protein chip that consists mostly of hcp lattice has better binding capability. SEM micrographs provide direct image evidence. These material tests summarize that the Ni-Co alloy coated protein microarray chip adsorbs His-tagged proteins through its surface morphology. Therefore, it can provide specific binding due to the affinity adsorption between the intermediate metals and the protein. PMID:24582262

Chang, Yaw-Jen; Ho, Ching-Yuan; Chang, Cheng-Hao

2014-04-01

254

Rapid tagging of endogenous mouse genes by recombineering and ES cell complementation of tetraploid blastocysts  

PubMed Central

The construction of knockin vectors designed to modify endogenous genes in embryonic stem (ES) cells and the generation of mice from these modified cells is time consuming. The timeline of an experiment from the conception of an idea to the availability of mature mice is at least 9 months. We describe a method in which this timeline is typically reduced to 3 months. Knockin vectors are rapidly constructed from bacterial artificial chromosome clones by recombineering followed by gap-repair (GR) rescue, and mice are rapidly derived by injecting genetically modified ES cells into tetraploid blastocysts. We also describe a tandem affinity purification (TAP)/floxed marker gene plasmid and a GR rescue plasmid that can be used to TAP tag any murine gene. The combination of recombineering and tetraploid blastocyst complementation provides a means for large-scale TAP tagging of mammalian genes. PMID:15356288

Zhou, Dewang; Ren, Jin-Xiang; Ryan, Thomas M.; Higgins, N. Patrick; Townes, Tim M.

2004-01-01

255

DICOM involving XML path-tag  

NASA Astrophysics Data System (ADS)

Digital Imaging and Communications in Medicine (DICOM) is a standard for handling, storing, printing, and transmitting information in medical imaging. XML (Extensible Markup Language) is a set of rules for encoding documents in machine-readable form which has become more and more popular. The combination of these two is very necessary and promising. Using XML tags instead of numeric labels in DICOM files will effectively increase the readability and enhance the clear hierarchical structure of DICOM files. However, due to the fact that the XML tags rely heavily on the orders of the tags, the strong data dependency has a lot of influence on the flexibility of inserting and exchanging data. In order to improve the extensibility and sharing of DICOM files, this paper introduces XML Path-Tag to DICOM. When a DICOM file is converted to XML format, adding simple Path-Tag into the DICOM file in place of complex tags will keep the flexibility of a DICOM file while inserting data elements and give full play to the advantages of the structure and readability of an XML file. Our method can solve the weak readability problem of DICOM files and the tedious work of inserting data into an XML file. In addition, we set up a conversion engine that can transform among traditional DICOM files, XML-DCM and XML-DCM files involving XML Path-Tag efficiently.

Zeng, Qiang; Yao, Zhihong; Liu, Lei

2011-03-01

256

Tags and seals for arms control verification  

SciTech Connect

Tags and seals have long been recognized as important tools in arms control. The trend in control of armaments is to limit militarily significant equipment that is capable of being verified through direct and cooperative means, chiefly on-site inspection or monitoring. Although this paper will focus on the CFE treaty, the role of tags and seals for other treaties will also be addressed. Published technology and concepts will be reviewed, based on open sources. Arms control verification tags are defined as unique identifiers designed to be tamper-revealing; in that respect, seals are similar, being used as indicators of unauthorized access. Tamper-revealing tags might be considered as single-point markers, seals as two-point couplings, and nets as volume containment. The functions of an arms control tag can be considered to be two-fold: to provide field verification of the identity of a treaty-limited item (TLI), and to have a means of authentication of the tag and its tamper-revealing features. Authentication could take place in the field or be completed elsewhere. For CFE, the goal of tags and seals can be to reduce the overall cost of the entire verification system.

DeVolpi, A.

1990-09-18

257

Enhanced UHF RFID tags for drug tracing.  

PubMed

Radio Frequency Identification (RFID) technology is playing a crucial role for item-level tracing systems in healthcare scenarios. The pharmaceutical supply chain is a fascinating application context, where RFID can guarantee transparency in the drug flow, supporting both suppliers and consumers against the growing counterfeiting problem. In such a context, the choice of the most adequate RFID tag, in terms of shape, frequency, size and reading range, is crucial. The potential presence of items containing materials hostile to the electromagnetic propagation exasperates the problem. In addition, the peculiarities of the different RFID-based checkpoints make even more stringent the requirements for the tag. In this work, the performance of several commercial UHF RFID tags in each step of the pharmaceutical supply chain has been evaluated, confirming the expected criticality. On such basis, a guideline for the electromagnetic design of new high-performance tags capable to overcome such criticalities has been defined. Finally, driven by such guidelines, a new enhanced tag has been designed, realized and tested. Due to patent pending issues, the antenna shape is not shown. Nevertheless, the optimal obtained results do not lose their validity. Indeed, on the one hand they demonstrate that high performance item level tracing systems can actually be implemented also in critical operating conditions. On the other hand, they encourage the tag designer to follow the identified guidelines so to realize enhanced UHF tags. PMID:22048779

Catarinucci, Luca; Colella, Riccardo; De Blasi, Mario; Patrono, Luigi; Tarricone, Luciano

2012-12-01

258

Semantic Stability in Social Tagging Streams  

E-print Network

One potential disadvantage of social tagging systems is that due to the lack of a centralized vocabulary, a crowd of users may never manage to reach a consensus on the description of resources (e.g., books, users or songs) on the Web. Yet, previous research has provided interesting evidence that the tag distributions of resources may become semantically stable over time as more and more users tag them. At the same time, previous work has raised an array of new questions such as: (i) How can we assess the semantic stability of social tagging systems in a robust and methodical way? (ii) Does semantic stabilization of tags vary across different social tagging systems and ultimately, (iii) what are the factors that can explain semantic stabilization in such systems? In this work we tackle these questions by (i) presenting a novel and robust method which overcomes a number of limitations in existing methods, (ii) empirically investigating semantic stabilization processes in a wide range of social tagging systems w...

Wagner, Claudia; Strohmaier, Markus; Huberman, Bernardo A

2013-01-01

259

Perfect tag identification protocol in RFID networks  

E-print Network

Radio Frequency IDentification (RFID) systems are becoming more and more popular in the field of ubiquitous computing, in particular for objects identification. An RFID system is composed by one or more readers and a number of tags. One of the main issues in an RFID network is the fast and reliable identification of all tags in the reader range. The reader issues some queries, and tags properly answer. Then, the reader must identify the tags from such answers. This is crucial for most applications. Since the transmission medium is shared, the typical problem to be faced is a MAC-like one, i.e. to avoid or limit the number of tags transmission collisions. We propose a protocol which, under some assumptions about transmission techniques, always achieves a 100% perfomance. It is based on a proper recursive splitting of the concurrent tags sets, until all tags have been identified. The other approaches present in literature have performances of about 42% in the average at most. The counterpart is a more sophistic...

Bonuccelli, Maurizio A; Martelli, Francesca

2008-01-01

260

Fabrication process for a flexible tag microlab  

NASA Astrophysics Data System (ADS)

The aim of this paper is to present an integrated process flow for a smart tag with integrated sensors and RFID communication, a Flexible Tag Microlab (FTM). The heart of the designed container tracing system is an RFID system (Reader + Tag) with gas sensing capabilities on board. In the former prototypes, the chemical sensors were integrated on the reader, whereas the tags where addressed like conventional RFID-tags containing also physical (temperature, humidity and light) sensors. However, this paper will show how the gas sensing reader functionalities are being transferred to the tag, reaching a flexible tag microlab, which represents a real innovation in the field of flexible labels. Key issues for the realisation of the FTM, such us flexible substrates and gas sensor integration technologies will be presented. The process flow employed for the two metal levels interconnect fabrication will be described in detail. The material used is the DuPont TM Pyralux (R) AP 8525R double-sided copper-clad laminate, formed by a Kapton foil with a copper layer on each side. The vias and windows openings are performed by femtosecond laser ablation. The copper interconnections are realized by photolithography and wet chemical etching. The MOX sensors hotplates specially developed to fulfil the FTM constrains in terms of low power consumption has been used to prove two integration technologies into the flexible substrates: Chip on Flex (COF) wire bonding and Anisotropic Conductive Adhesive (ACA) flip chip bonding. Both technologies will be compared and benchmarked for future product developments.

Abad, E.; Mazzolai, B.; Juarros, A.; Gómez, D.; Mondini, A.; Sayhan, I.; Krenkow, A.; Becker, Th.

2007-05-01

261

Tag retention, growth, and survival of red swamp crayfish Procambarus clarkii marked with coded wire tags  

USGS Publications Warehouse

Juvenile red swamp crayfish (or crawfish), Procambarus clarkii (20-41 mm in total length) were collected from a crayfish culture pond by dipnetting and tagged with sequentially numbered, standard length, binary-coded wire tags. Four replicates of 50 crayfish were impaled perpendicular to the long axis of the abdomen with a fixed needle. Tags were injected transversely into the ventral surface of the first or second abdominal segment and were imbedded in the musculature just beneath the abdominal sternum. Tags were visible upon inspection. Additionally, two replicates of 50 crayfish were not tagged and were used as controls. Growth, survival, and tag retention were evaluated after 7 d in individual containers, after 100 d in aquaria, and after 200 d in field cages. Tag retention during each sample period was 100%, and average mortality of tagged crayfish within 7 d of tagging was 1%. Mortality during the remainder of the study was high (75-91%) but was similar between treatment and control samples. Most of the deaths were probably due to cannibalism. Average total length increased threefold during the course of the study, and crayfish reached maturity. Because crayfish were mature by the end of the study, we concluded that the coded wire tag was retained through the life history of the crayfish.

Isely, J.J.; Eversole, A.G.

1998-01-01

262

Convergent evidence identifying MAP\\/microtubule affinity-regulating kinase 1 (MARK1) as a susceptibility gene for autism  

Microsoft Academic Search

Autism spectrum disorders (ASDs) are common, heritable, but genetically heterogeneous neurodevelopmen- tal conditions. We recently defined a susceptibility locus for ASDs on chromosome 1q41-q42. High- resolution single-nucleotide polymorphisms (126 SNPs) genotyping across the chromosome 1q41-q42 region, followed by a MARK1 (microtubule affinity-regulating kinase 1)-tagged-SNP association study in 276 families with autism from the Autism Genetic Research Exchange, showed that several

Gilles Maussion; J erome Carayol; Aude-Marie Lepagnol-Bestel; Frederic Tores; Yann Loe-Mie; Ulla Milbreta; Francis Rousseau; Karine Fontaine; Julie Renaud; Jean-Marie Moalic; Anne Philippi; Alain Chedotal; Philip Gorwood; Nicolas Ramoz; Jorg Hager; Michel Simonneau

2008-01-01

263

Stochastic modeling of affinity adsorption.  

PubMed

A stochastic model is described that allows surface proximity and packing effects to be incorporated into predictions of adsorption kinetics and equilibrium of affinity adsorption. Equilibrium predictions show that, depending on conditions chosen, the results obtained for equilibrium conditions can exhibit either a Freundlich- or a Langmuir-type relationship. Under conditions of surface density imposed adsorption constraints, the time taken for equilibrium to be reached increases as the "off" constant is decreased. This suggests that for resins having a high immobilized ligand density binding kinetics may be more highly limited by the "off" constant than by mass transfer limitations. PMID:11386880

Hubble, J

2001-01-01

264

Electrochemical immobilization of Fluorescent labelled probe molecules on a FTO surface for affinity detection based on photo-excited current  

NASA Astrophysics Data System (ADS)

Photo-excited current can be generated at a molecular interface between a photo-excited molecules and a semi-conductive material in appropriate condition. The system has been recognized for promoting photo-energy devices such as an organic dye sensitized solar-cell. The photo-current generated reactions are totally dependent on the interfacial energy reactions, which are in a highly fluctuated interfacial environment. The authors investigated the photo-excited current reaction to develop a smart affinity detection method. However, in order to perform both an affinity reaction and a photo-excited current reaction at a molecular interface, ordered fabrications of the functional (affinity, photo-excitation, etc.) molecules layer on a semi-conductive surface is required. In the present research, we would like to present the fabrication and functional performance of photo-excited current-based affinity assay device and its application for detection of endocrine disrupting chemicals. On the FTO surface, fluorescent pigment labelled affinity peptide was immobilized through the EC tag (electrochemical-tag) method. The modified FTO produced a current when it was irradiated with diode laser light. However, the photo current decreased drastically when estrogen (ES) coexisted in the reaction solution. In this case, immobilized affinity probe molecules formed a complex with ES and estrogen receptor (ER). The result strongly suggests that the photo-excited current transduction between probe molecule-labelled cyanine pigment and the FTO surface was partly inhibited by a complex that formed at the affinity oligo-peptide region in a probe molecule on the FTO electrode. The bound bulky complex may act as an impediment to perform smooth transduction of photo-excited current in the molecular interface. The present system is new type of photo-reaction-based analysis. This system can be used to perform simple high-sensitive homogeneous assays.

Haruyama, Tetsuya; Wakabayashi, Ryo; Cho, Takeshi; Matsuyama, Sho-taro

2011-10-01

265

Comparative Performance of Acoustic-tagged and PIT-tagged Juvenile Salmonids  

SciTech Connect

Numerous research tools and technologies are currently being used to evaluate fish passage and survival to determine the impacts of the Federal Columbia River Power System (FCRPS) on endangered and threatened juvenile salmonids, including PIT tags, balloon tags, hydroacoustic evaluations, radio telemetry, and acoustic telemetry. Each has advantages and disadvantages, but options are restricted in some situations because of limited capabilities of a specific technology, lack of detection capability downstream, or availability of adequate numbers of fish. However, there remains concern about the comparative effects of the tag or the tagging procedure on fish performance. The recently developed Juvenile Salmonid Acoustic Telemetry System (JSATS) acoustic transmitter is the smallest active acoustic tag currently available. The goal of this study was to determine whether fish tagged with the JSATS acoustic-telemetry tag can provide unbiased estimates of passage behavior and survival within the performance life of the tag. We conducted both field and laboratory studies to assess tag effects. For the field evaluation we released a total of 996 acoustic-tagged fish in conjunction with 21,026 PIT-tagged fish into the tailrace of Lower Granite Dam on 6 and 13 May. Travel times between release and downstream dams were not significantly different for the majority of the reaches between acoustic-tagged and PIT-tagged fish. In addition to the field evaluation, a series of laboratory experiments were conducted to determine if growth and survival of juvenile Chinook salmon surgically implanted with acoustic transmitters is different than untagged or PIT tagged juvenile Chinook salmon. Only yearling fish with integrated and non-integrated transmitters experienced mortalities, and these were low (<4.5%). Mortality among sub-yearling control and PIT-tag treatments ranged up to 7.7% while integrated and non-integrated treatments had slightly higher rates (up to 8.3% and 7.9% respectively). No acoustic transmitters were shed by yearling fish during the course of the 90 day study. Up to 7.8% of subyearling fish expelled transmitters. Tags were expelled from 5 to 63 days post-surgery. The average time to expulsion was 27 days; few fish expelled transmitters within 14 days of implantation or less. Histological results suggest that inflammation associated with implantation of an acoustic transmitter can produce fibrous tissue which can invade and possibly damage internal organs soon after implantation. Reactions severe enough to damage organs however, were limited to only ~20% of subyearling Chinook salmon, all of which were under 101mm and 12g at tagging. The infiltration of the fibrous tissue into organs was observed most often in fish held for 21 days and appeared to decrease in subsequent holding times.

Hockersmith, Eric E.; Brown, Richard S.; Liedtke, Theresa L.

2008-02-01

266

Stable isotope-coded proteomic mass spectrometry  

Microsoft Academic Search

Developing the ability to quantify changes in protein abundance between cells subjected to a variety of physiological and environmental conditions is an extremely active area of proteome research. Although advances in chromatography, mass spectrometry instrumentation, and bioinformatics have contributed to producing a viable method for comparative proteome-wide analyses, the highest precision of quantitation is based, in part, upon improved methods

Michael B. Goshe; Richard D. Smith

2003-01-01

267

Indian Craniometric Variability and Affinities  

PubMed Central

Recently published craniometric and genetic studies indicate a predominantly indigenous ancestry of Indian populations. We address this issue with a fuller coverage of Indian craniometrics than any done before. We analyse metrical variability within Indian series, Indians' sexual dimorphism, differences between northern and southern Indians, index-based differences of Indian males from other series, and Indians' multivariate affinities. The relationship between a variable's magnitude and its variability is log-linear. This relationship is strengthened by excluding cranial fractions and series with a sample size less than 30. Male crania are typically larger than female crania, but there are also shape differences. Northern Indians differ from southern Indians in various features including narrower orbits and less pronounced medial protrusion of the orbits. Indians resemble Veddas in having small crania and similar cranial shape. Indians' wider geographic affinities lie with “Caucasoid” populations to the northwest, particularly affecting northern Indians. The latter finding is confirmed from shape-based Mahalanobis-D distances calculated for the best sampled male and female series. Demonstration of a distinctive South Asian craniometric profile and the intermediate status of northern Indians between southern Indians and populations northwest of India confirm the predominantly indigenous ancestry of northern and especially southern Indians. PMID:24455409

Raghavan, Pathmanathan; Bulbeck, David; Pathmanathan, Gayathiri; Rathee, Suresh Kanta

2013-01-01

268

Multi-exemplar affinity propagation.  

PubMed

The affinity propagation (AP) clustering algorithm has received much attention in the past few years. AP is appealing because it is efficient, insensitive to initialization, and it produces clusters at a lower error rate than other exemplar-based methods. However, its single-exemplar model becomes inadequate when applied to model multisubclasses in some situations such as scene analysis and character recognition. To remedy this deficiency, we have extended the single-exemplar model to a multi-exemplar one to create a new multi-exemplar affinity propagation (MEAP) algorithm. This new model automatically determines the number of exemplars in each cluster associated with a super exemplar to approximate the subclasses in the category. Solving the model is NP-hard and we tackle it with the max-sum belief propagation to produce neighborhood maximum clusters, with no need to specify beforehand the number of clusters, multi-exemplars, and superexemplars. Also, utilizing the sparsity in the data, we are able to reduce substantially the computational time and storage. Experimental studies have shown MEAP's significant improvements over other algorithms on unsupervised image categorization and the clustering of handwritten digits. PMID:23868781

Wang, Chang-Dong; Lai, Jian-Huang; Suen, Ching Y; Zhu, Jun-Yong

2013-09-01

269

Multi-Exemplar Affinity Propagation.  

PubMed

Affinity Propagation (AP) clustering algorithm has received much attention in the past few years. AP is appealing because it is efficient, insensitive to initialization, and it produces clusters at a lower error rate than other exemplar-based methods. However, its single-exemplar model becomes inadequate when applied to model multi-subclasses in some situations such as scene analysis and character recognition. To remedy this deficiency, we have extended the single-exemplar model to a multi-exemplar one to create a new Multi-Exemplar Affinity Propagation (MEAP) algorithm. This new model determines automatically the number of exemplars in each cluster associated with a super exemplar to approximate the subclasses in the category. Solving the model is NP-hard and we tackle it with the max-sum belief propagation to produce neighborhood maximum clusters, with no need to specify beforehand the number of clusters, multi-exemplars, and super-exemplars. Also, utilizing the sparsity in the data, we are able to reduce substantially the computational time and storage. Experimental studies have shown MEAP's significant improvements over other algorithms on unsupervised image categorization and the clustering of handwritten digits. PMID:23358283

Wang, Chang-Dong; Lai, Jian-Huang; Suen, Ching Y; Zhu, Jun-Yong

2013-01-24

270

Evacuation of Passive Integrated Transponder (PIT) Tags from Northern Pikeminnow Consuming Tagged Juvenile Chinook Salmon  

USGS Publications Warehouse

Prey fish implanted with passive integrated transponder (PIT) tags can be used in predation studies if the timing of tag evacuation from the predators is understood. Laboratory experiments were conducted to determine how PIT tags in juvenile Chinook salmon Oncorhynchus tshawytscha that were consumed by northern pikeminnow Ptychocheilus oregonensis were evacuated in relation to various parameters. The rate of evacuation was directly related to temperature, while predator size and the number of prey consumed had less effect on the timing of tag evacuation. A power model was fitted to predict the proportion of tags expected to be evacuated at different intervals after ingestion. These results could be used in planning field or laboratory predation experiments with PIT-tagged prey fish.

Petersen, J.H.; Barfoot, C.A.

2003-01-01

271

The tandem affinity purification method: an efficient system for protein complex purification and protein interaction identification.  

PubMed

Isolation and identification of protein partners in multi-protein complexes are important in gaining further insights into the cellular roles of proteins and determining the possible mechanisms by which proteins have an effect in the molecular environment. The tandem affinity purification (TAP) method was originally developed in yeast for the purification of protein complexes and identification of protein-protein interactions. With modifications to this method and many variations in the original tag made over the past few years, the TAP system could be applied in mammalian, plant, bacteria and other systems for protein complex analysis. In this review, we describe the application of the TAP method in various organisms, the modification in the tag, the disadvantages, the developments and the future prospects of the TAP method. PMID:20399864

Xu, Xiaoli; Song, Yuan; Li, Yuhua; Chang, Jianfeng; Zhang, Hua; An, Lizhe

2010-08-01

272

Time-Tag Generation Script  

NASA Technical Reports Server (NTRS)

Time-Tag Generation Script (TTaGS) is an application program, written in the AWK scripting language, for generating commands for aiming one Ku-band antenna and two S-band antennas for communicating with spacecraft. TTaGS saves between 2 and 4 person-hours per every 24 hours by automating the repetitious process of building between 150 and 180 antenna-control commands. TTaGS reads a text database of communication satellite schedules and a text database of satellite rise and set times and cross-references items in the two databases. It then compares the scheduled start and stop with the geometric rise and set to compute the times to execute antenna control commands. While so doing, TTaGS determines whether to generate commands for guidance, navigation, and control computers to tell them which satellites to track. To help prevent Ku-band irradiation of the Earth, TTaGS accepts input from the user about horizon tolerance and accordingly restricts activation and effects deactivation of the transmitter. TTaGS can be modified easily to enable tracking of additional satellites and for such other tasks as reading Sun-rise/set tables to generate commands to point the solar photovoltaic arrays of the International Space Station at the Sun.

Jackson, Dan E.

2010-01-01

273

TagSense: a smartphone-based approach to automatic image tagging  

Microsoft Academic Search

Mobile phones are becoming the convergent platform for personal sensing, computing, and communication. This paper attempts to exploit this convergence towards the problem of automatic image tagging. We envision TagSense, a mobile phone based collaborative system that senses the people, activity, and context in a picture, and merges them carefully to create tags on-the-fly. The main challenge pertains to discriminating

Chuan Qin; Xuan Bao; Romit Roy Choudhury; Srihari Nelakuditi

2011-01-01

274

50 CFR 20.81 - Tagging requirement.  

Code of Federal Regulations, 2013 CFR

...EXPORTATION, AND IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) MIGRATORY BIRD HUNTING Migratory Bird Preservation Facilities § 20.81 Tagging requirement. No migratory bird preservation facility shall receive or have in custody any...

2013-10-01

275

50 CFR 20.81 - Tagging requirement.  

Code of Federal Regulations, 2012 CFR

...EXPORTATION, AND IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) MIGRATORY BIRD HUNTING Migratory Bird Preservation Facilities § 20.81 Tagging requirement. No migratory bird preservation facility shall receive or have in custody any...

2012-10-01

276

Modeling social tagging using latent interaction potential  

NASA Astrophysics Data System (ADS)

Modeling social tagging plays a critical role in identifying statistical regularities and structural principles common to social tagging systems. Existing modeling approaches only consider imitations or background knowledge of users. However, common interests among users are ignored. In this paper, latent interactions are applied to present the common interests, and dynamic patterns in empirical data are investigated. Furthermore, the latent interaction driven model (LIDM) is proposed to model social tagging. Experimental results show that the tag frequency distribution generated by LIDM is consistent with that in real-world data. Moreover, the latent interaction graph generated by LIDM has a higher average clustering coefficient and lower average shortest path compared with that generated by preferential attachment methods. This demonstrates that LIDM outperforms traditional methods.

Wu, Zhenyu; Zou, Ming

2014-11-01

277

50 CFR 20.36 - Tagging requirement.  

Code of Federal Regulations, 2013 CFR

...cleaning, processing, shipping, transportation, or storage (including temporary storage), or for the purpose of having taxidermy services performed, unless such birds have a tag attached, signed by the hunter, stating his address, the total number...

2013-10-01

278

50 CFR 20.36 - Tagging requirement.  

Code of Federal Regulations, 2014 CFR

...cleaning, processing, shipping, transportation, or storage (including temporary storage), or for the purpose of having taxidermy services performed, unless such birds have a tag attached, signed by the hunter, stating his address, the total number...

2014-10-01

279

User Interface Program for secure electronic tags  

SciTech Connect

This report summarizes and documents the efforts of Argonne National Laboratory (ANL) in developing a secure tag communication user interface program comprising a tag monitor and a communication tool. This program can perform the same functions as the software that was developed at the Lawrence Livermore National Laboratory (LLNL), but it is enhanced with a user-friendly screen. It represents the first step in updating the TRANSCOM Tracking System (TRANSCOM) by incorporating a tag communication screen menu into the main menu of the TRANSCOM user program. A working version of TRANSCOM, enhanced with ANL secure-tag graphics, will strongly support the Department of Energy Warhead Dismantlement/Special Nuclear Materials Control initiatives. It will allow commercial satellite tracking of the movements and operational activities of treaty-limited items and transportation vehicles throughout Europe and the former USSR, as well as the continental US.

Cai, Y.; Koehl, E.R.; Carlson, R.D.; Raptis, A.C.

1995-05-01

280

Lanthanide-tagged proteins – An illuminating partnership  

E-print Network

Lanthanide-tagged proteins are valuable for exploiting the unique properties of Ln ions for investigating protein structure, function, and dynamics. Introduction of the Ln into the target is accomplished via chemical ...

Imperiali, Barbara

281

Multi-Tag RFID Systems Leonid Bolotnyy  

E-print Network

is the primary objective of radio frequency identification (RFID) technology. Yet, a recent major study by Wal-Mart with multiple tags to address the fundamental issue of object detectability. We show that this strategy

Robins, Gabriel

282

Conformal field theory on affine Lie groups  

SciTech Connect

Working directly on affine Lie groups, we construct several new formulations of the WZW model, the gauged WZW model, and the generic affine-Virasoro action. In one formulation each of these conformal field theories (CFTs) is expressed as a one-dimensional mechanical system whose variables are coordinates on the affine Lie group. When written in terms of the affine group element, this formulation exhibits a two-dimensional WZW term. In another formulation each CFT is written as a two-dimensional field theory, with a three- dimensional WZW term, whose fields are coordinates on the affine group. On the basis of these equivalent formulations, we develop a translation dictionary in which the new formulations on the affine Lie group are understood as mode formulations of the conventional formulations on the Lie group. Using this dictionary, we also express each CFT as a three-dimensional field theory on the Lie group with a four-dimensional WZW term. 36 refs.

Clubok, K.S.

1996-04-01

283

A method for affine invariant curve smoothing  

NASA Astrophysics Data System (ADS)

This paper proposes a new curve smoothing method invariant to affine transformation. Curve smoothing is one of the important challenges in computer vision as a procedure for noise suppression in shape analysis such as Curvature Scale Space (CSS). Currently, Gaussian filtering is widely used among a lot of smoothing methods. However Gaussian filtering is not affine invariant. This paper proposes a new method for curve smoothing that is invariant under affine transformation such that area of any region in the image does not change. Specifically, we introduce an affine invariant evaluate function with a metric tensor. The original curve is smoothed by minimizing the evaluation function. We mathematically prove that this method is affine invariant. Further, experimental results show that the proposed method is almost never affected by affine transformation different from usual Gaussian filtering. In the proposed method, processing results are expected to be not affected much by variation of the viewpoint.

Nishida, Takahiro; Toriu, Takashi

2014-01-01

284

Lock-Out / Tag-Out  

NSDL National Science Digital Library

Tags and locks can be the last line of defense against machinery accidents. This MATEC module leaves your learners solidly grounded in OSHA's six-step lockout/tagout procedures for preventing unexpected start-ups of equipment. They learn how to use energy-isolating devices and how to safely remove locks and tags. They can demonstrate their facility with the OHSA standards using a machine on the manufacturing floor.

285

PolemicTweet: Video Annotation and Analysis through Tagged Tweets  

E-print Network

PolemicTweet: Video Annotation and Analysis through Tagged Tweets Samuel Huron1,1,2 , Petra tagging and analysis. Annotating and tag- ging videos manually is a boring and time-consuming process. Yet: Backchannel, Video annotation, Crowdsourcing, Video analysis, Live tagging. 1 Introduction Fig. 1. The three

Paris-Sud XI, Université de

286

Improving security and usability of low cost RFID tags  

Microsoft Academic Search

Low cost RFID tags pose unique security challenges. Data tampering is one of such challenges that need to be addressed. In this paper, we propose a tamper detection solution for the EPC-Class1 Generation2 tag (a low cost passive RFID tag) based on a cryptographic PRNG (a pseudo random number generator for low cost RFID tags) function called LAMED and the

Ali N M Noman; Carlisle Adams

2011-01-01

287

Strep-tagged Protein Purification For expressing, purifying, and detecting  

E-print Network

-NTA Magnetic Agarose Beads 39 Appendix B: Composition of Buffers 41 Appendix C: Regeneration of StrepStrep-tagged Protein Purification Handbook For expressing, purifying, and detecting proteins carrying a Strep-tag® II or a 6xHis tag and a Strep-tag II Two-step protein purification system His·Strep p

Lebendiker, Mario

288

Social Tagging, Guppy Effect and the Role of Interference  

E-print Network

-occurrence of tags, used for example by Flickr2 [10]. For instance, the url http://www.flickr.com/ photos/tags/goldfish/clusters/ presents clusters of images related to the tag goldfish. One of those clusters contains the tags fish

Boschetti, Fabio

289

Lightweight Authentication Protocols for Low-Cost RFID Tags  

Microsoft Academic Search

Providing security in low-cost RFID tags is a challenging task because tags are highly resource con- strained and cannot support strong cryptography. Special lightweight algorithms and protocols need to be designed that take into account the limitations of the tags. In this paper, we propose a set of extremely lightweight tag authentication protocols. We also provide an analysis of the

Istvan Vajda

2003-01-01

290

Theory and Measurement of Backscattering from RFID Tags  

E-print Network

Theory and Measurement of Backscattering from RFID Tags Pavel V. Nikitin and K. V. S. Rao Intermec backscattering from RFID tags and for calculating a tag radar cross-section (RCS). We derive a theoretical formula for RCS of an RFID tag with a minimum scattering antenna and describe an experimental measurement

Hochberg, Michael

291

Theory and measurement of backscattering from RFID tags  

Microsoft Academic Search

This paper presents a method for measuring signal backscattering from RFID tags, and for calculating a tag's radar cross section (RCS). We derive a theoretical formula for the RCS of an RFID tag with a minimum-scattering antenna. We describe an experimental measurement technique, which involves using a network analyzer connected to an anechoic chamber with and without the tag. The

Pavel V. Nikitin; K. V. S. Rao

2006-01-01

292

The Internet of Tags: Energy-Harvesting Adaptive Algorithms  

E-print Network

The Internet of Tags: Energy-Harvesting Adaptive Algorithms Robert Margolies Ph.D. Candidate a top-down approach and develop energy harvesting adaptive algorithms to support the Internet of Tags (IoTags). We believe that IoTags will be a key component of the Internet of Things (IoT). In the near

Hone, James

293

Three types of RFID tags Passive / Active / Semi-Active  

E-print Network

cage pants pocket RFID-enabled passport RFID-tagged laundry Flexible washer-safe RFID tag PricingThree types of RFID tags · Passive / Active / Semi-Active · up to 19m range for UHF Computer GeneralizedGeneralized ""YokingYoking--ProofsProofs"" for a Group of RFID Tagsfor a Group of RFID Tags RFID

Robins, Gabriel

294

SNAP-tagging the retrograde route.  

PubMed

We have developed a chemical biology strategy to identify proteins that follow the retrograde transport route from the plasma membrane to the Golgi apparatus, via endosomes. The general principle is the following: plasma membrane proteins are covalently tagged with a first probe. Only the ones that are then transported to trans-Golgi/TGN membranes are covalently bound to a capture reagent that has been engineered into this compartment. Specifically, the first probe is benzylguanine (BG) that is conjugated onto primary amino groups of plasma-membrane proteins. The capture reagent includes an O(6)-alkylguanine-DNA alkyltransferase-derived fragment, the SNAP-tag, which forms a covalent linkage with BG. The SNAP-tag is fused to the GFP-tagged Golgi membrane anchor from galactosyl transferase for proper targeting to trans-Golgi/TGN membranes. Cell-surface BG-tagged proteins that are transported to trans-Golgi/TGN membranes (i.e., that are retrograde cargoes) are thereby covalently captured by the SNAP-tag fusion protein. For identification, the latter is immunopurified using GFP-Trap, and associated retrograde cargo proteins are identified by mass spectrometry. We here provide a step-by-step protocol of this method. PMID:24295305

Johannes, Ludger; Shafaq-Zadah, Massiullah

2013-01-01

295

Neural net controlled tag gas sampling system for nuclear reactors  

DOEpatents

A method and system are disclosed for providing a tag gas identifier to a nuclear fuel rod and analyze escaped tag gas to identify a particular failed nuclear fuel rod. The method and system include disposing a unique tag gas composition into a plenum of a nuclear fuel rod, monitoring gamma ray activity, analyzing gamma ray signals to assess whether a nuclear fuel rod has failed and is emitting tag gas, activating a tag gas sampling and analysis system upon sensing tag gas emission from a failed nuclear rod and evaluating the escaped tag gas to identify the particular failed nuclear fuel rod. 12 figs.

Gross, K.C.; Laug, M.T.; Lambert, J.B.; Herzog, J.P.

1997-02-11

296

Neural net controlled tag gas sampling system for nuclear reactors  

DOEpatents

A method and system for providing a tag gas identifier to a nuclear fuel rod and analyze escaped tag gas to identify a particular failed nuclear fuel rod. The method and system include disposing a unique tag gas composition into a plenum of a nuclear fuel rod, monitoring gamma ray activity, analyzing gamma ray signals to assess whether a nuclear fuel rod has failed and is emitting tag gas, activating a tag gas sampling and analysis system upon sensing tag gas emission from a failed nuclear rod and evaluating the escaped tag gas to identify the particular failed nuclear fuel rod.

Gross, Kenneth C. (Bolingbrook, IL); Laug, Matthew T. (Idaho Fall, ID); Lambert, John D. B. (Wheaton, IL); Herzog, James P. (Downers Grove, IL)

1997-01-01

297

Non-affine deformations in polymer hydrogels  

PubMed Central

Most theories of soft matter elasticity assume that the local strain in a sample after deformation is identical everywhere and equal to the macroscopic strain, or equivalently that the deformation is affine. We discuss the elasticity of hydrogels of crosslinked polymers with special attention to affine and non-affine theories of elasticity. Experimental procedures to measure non-affine deformations are also described. Entropic theories, which account for gel elasticity based on stretching out individual polymer chains, predict affine deformations. In contrast, simulations of network deformation that result in bending of the stiff constituent filaments generally predict non-affine behavior. Results from experiments show significant non-affine deformation in hydrogels even when they are formed by flexible polymers for which bending would appear to be negligible compared to stretching. However, this finding is not necessarily an experimental proof of the non-affine model for elasticity. We emphasize the insights gained from experiments using confocal rheoscope and show that, in addition to filament bending, sample micro-inhomogeneity can be a significant alternative source of non-affine deformation. PMID:23002395

Wen, Qi; Basu, Anindita; Janmey, Paul A.; Yodh, A. G.

2012-01-01

298

Maxwell-affine gauge theory of gravity  

E-print Network

Maxwell extension of affine algebra with additional tensorial generators is given. Using the methods of nonlinear realizations, we found the transformation rules for group parameters and corresponding generators. Gauging the Maxwell-affine algebra we presented two possible invariant actions for gravity: one is the first order and the other one is the second order in affine curvature. We noticed that equations of motion for the action, second order in affine curvature, lead to the generalized Bianchi identities on the choice of appropriate coefficients for a particular solution of the constraint equation.

Cebecio?lu, O

2015-01-01

299

Structural determinants of sigma receptor affinity  

SciTech Connect

The structural determinants of sigma receptor affinity have been evaluated by examining a wide range of compounds related to opioids, neuroleptics, and phenylpiperidine dopaminergic structures for affinity at sigma receptor-binding sites labeled with (+)-(/sup 3/H)3-PPP. Among opioid compounds, requirements for sigma receptor affinity differ strikingly from the determinants of affinity for conventional opiate receptors. Sigma sites display reverse stereoselectivity to classical opiate receptors. Multi-ringed opiate-related compounds such as morphine and naloxone have negligible affinity for sigma sites, with the highest sigma receptor affinity apparent for benzomorphans which lack the C ring of opioids. Highest affinity among opioids and other compounds occurs with more lipophilic N-substituents. This feature is particularly striking among the 3-PPP derivatives as well as the opioids. The butyrophenone haloperidol is the most potent drug at sigma receptors we have detected. Among the series of butyrophenones, receptor affinity is primarily associated with the 4-phenylpiperidine moiety. Conformational calculations for various compounds indicate a fairly wide range of tolerance for distances between the aromatic ring and the amine nitrogen, which may account for the potency at sigma receptors of structures of considerable diversity. Among the wide range of structures that bind to sigma receptor-binding sites, the common pharmacophore associated with high receptor affinity is a phenylpiperidine with a lipophilic N-substituent.

Largent, B.L.; Wikstroem, H.G.; Gundlach, A.L.; Snyder, S.H.

1987-12-01

300

The maximal affinity of ligands  

PubMed Central

We explore the question of what are the best ligands for macromolecular targets. A survey of experimental data on a large number of the strongest-binding ligands indicates that the free energy of binding increases with the number of nonhydrogen atoms with an initial slope of ??1.5 kcal/mol (1 cal = 4.18 J) per atom. For ligands that contain more than 15 nonhydrogen atoms, the free energy of binding increases very little with relative molecular mass. This nonlinearity is largely ascribed to nonthermodynamic factors. An analysis of the dominant interactions suggests that van der Waals interactions and hydrophobic effects provide a reasonable basis for understanding binding affinities across the entire set of ligands. Interesting outliers that bind unusually strongly on a per atom basis include metal ions, covalently attached ligands, and a few well known complexes such as biotin–avidin. PMID:10468550

Kuntz, I. D.; Chen, K.; Sharp, K. A.; Kollman, P. A.

1999-01-01

301

Identification of Signaling Protein Complexes by Parallel Affinity Precipitation Coupled with Mass Spectrometry  

PubMed Central

Protein–protein interactions play a pivotal role in both inter- and intra-cellular signaling. Identification of signaling protein complexes can thus shed important new insights into cell communications. We developed a parallel affinity precipitation protocol to overcome the disadvantages of the tandem affinity purification procedure, such as the potential disruption of target protein conformation, subcellular localization or function by epitope tags, the potential need of large amounts of cell culture or generation of stable cell lines, and relatively long duration the two-step precipitation takes. This new simplified assay of protein interaction is quick, economic and specific. This paper describes the details in the design and method of the assay. PMID:24839392

Lu, Heng; Lin, Qishan; Zhao, Jihe

2014-01-01

302

NMFS Cooperative SharkTagging Program, 1962-93: An Atlas of SharkTag and Recapture Data  

E-print Network

the Department of Interior's U.S. Fish and Wildlife Service (USFWS). During the late 1950's and early 1960's. The survey resulted in the capture of over 300 sharks, includ ing white sharks, Carcharodon car charias tag (Jumbo Roto tag) and a dart tag ("M" tag) (Fig. 1). The Rototag is a two-piece, plastic cattle ear

303

Proteomic Studies of Syk-Interacting Proteins Using a Novel Amine-Specific Isotope Tag and GFP Nanotrap  

NASA Astrophysics Data System (ADS)

Green fluorescent protein (GFP) and variants have become powerful tools to study protein localization, interactions, and dynamics. We present here a mass spectrometry-based proteomics strategy to examine protein-protein interactions using anti-GFP single-chain antibody VHH in a combination with a novel stable isotopic labeling reagent, isotope tag on amino groups (iTAG). We demonstrate that the single-chain VHH (GFP nanotrap) allows us to identify interacting partners of the Syk protein-tyrosine kinase bearing a GFP epitope tag with high efficiency and high specificity. Interacting proteins identified include CrkL, BLNK, ?- and ?-tubulin, Csk, RanBP5 and DJ-1. The iTAG reagents were prepared with simple procedures and characterized with high accuracy in the determination of peptides in model peptide mixtures and as well as in complex mixture. Applications of the iTAG method and GFP nanotrap to an analysis of the nucleocytoplasmic trafficking of Syk led to the identification of location-specific associations between Syk and multiple proteins. While the results reveal that the new quantitative proteomic strategy is generally applicable to integrate protein interaction data with subcellular localization, extra caution should be taken in evaluating the results obtained by such affinity purification strategies as many interactions appear to occur following cell lysis.

Galan, Jacob A.; Paris, Leela L.; Zhang, Hua-jie; Adler, Jacob; Geahlen, Robert L.; Tao, W. Andy

2011-02-01

304

Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice  

NASA Astrophysics Data System (ADS)

Proteomic approaches require simple and efficient protein purification methodologies that are amenable to high throughput. Biotinylation is an attractive approach for protein complex purification due to the very high affinity of avidin/streptavidin for biotinylated templates. Here, we describe an approach for the single-step purification of transcription factor complex(es) based on specific in vivo biotinylation. We expressed the bacterial BirA biotin ligase in mammalian cells and demonstrated very efficient biotinylation of a hematopoietic transcription factor bearing a small (23-aa) artificial peptide tag. Biotinylation of the tagged transcription factor altered neither the factor's protein interactions or DNA binding properties in vivo nor its subnuclear distribution. Using this approach, we isolated the biotin-tagged transcription factor and at least one other known interacting protein from crude nuclear extracts by direct binding to streptavidin beads. Finally, this method works efficiently in transgenic mice, thus raising the prospect of using biotinylation tagging in protein complex purification directly from animal tissues. Therefore, BirA-mediated biotinylation of tagged proteins provides the basis for the single-step purification of proteins from mammalian cells.

de Boer, Ernie; Rodriguez, Patrick; Bonte, Edgar; Krijgsveld, Jeroen; Katsantoni, Eleni; Heck, Albert; Grosveld, Frank; Strouboulis, John

2003-06-01

305

Proteomic Studies of Syk-Interacting Proteins Using a Novel Amine-Specific Isotope Tag and GFP Nanotrap  

PubMed Central

Green fluorescent protein (GFP) and variants have become powerful tools to study protein localization, interactions, and dynamics. We present here a mass spectrometry-based proteomics strategy to examine protein–protein interactions using anti-GFP single-chain antibody VHH in a combination with a novel stable isotopic labeling reagent, isotope tag on amino groups (iTAG). We demonstrate that the single-chain VHH (GFP nanotrap) allows us to identify interacting partners of the Syk protein-tyrosine kinase bearing a GFP epitope tag with high efficiency and high specificity. Interacting proteins identified include CrkL, BLNK, ?- and ?-tubulin, Csk, RanBP5 and DJ-1. The iTAG reagents were prepared with simple procedures and characterized with high accuracy in the determination of peptides in model peptide mixtures and as well as in complex mixture. Applications of the iTAG method and GFP nanotrap to an analysis of the nucleocytoplasmic trafficking of Syk led to the identification of location-specific associations between Syk and multiple proteins. While the results reveal that the new quantitative proteomic strategy is generally applicable to integrate protein interaction data with subcellular localization, extra caution should be taken in evaluating the results obtained by such affinity purification strategies as many interactions appear to occur following cell lysis. PMID:21472591

Galan, Jacob A.; Paris, Leela L.; Zhang, Hua-jie; Adler, Jacob; Geahlen, Robert L.; Tao, W. Andy

2011-01-01

306

Magnetic resonance cardiac tagging: visualization of LV function parameters  

Microsoft Academic Search

Magnetic Resonance Imaging (MRI) tissue tagging allows the non-invasive placement of magnetic markers into the myocardium. These tags appear as black lines and remain visible on the myocardium during the cardiac cycle. A semi-automatic segmentation tool is used to delineate the tags. A tagging analysis program calculates from these time-varying tag coordinates several LV functional parameters: e.g. radius of curvature,

P. Plets; F. Rademakers; J. Van Cleynenbreugel; J. Bogaert; P. Suetens; G. Marchal

1996-01-01

307

Immunopaleontology reveals how affinity enhancement is achieved during affinity maturation of antibodies to influenza virus  

E-print Network

The Abs made by B lymphocytes on first encountering an antigen bind it with low intrinsic affinity, and, over time, the average affinity of the Abs made against that antigen gradually increases. These changes, known as ...

Eisen, Herman N.

308

Associated Particle Tagging (APT) in Magnetic Spectrometers  

SciTech Connect

Summary In Brief The Associated Particle Tagging (APT) project, a collaboration of Pacific Northwest National Laboratory (PNNL), Idaho National Laboratory (INL) and the Idaho State University (ISU)/Idaho Accelerator Center (IAC), has completed an exploratory study to assess the role of magnetic spectrometers as the linchpin technology in next-generation tagged-neutron and tagged-photon active interrogation (AI). The computational study considered two principle concepts: (1) the application of a solenoidal alpha-particle spectrometer to a next-generation, large-emittance neutron generator for use in the associated particle imaging technique, and (2) the application of tagged photon beams to the detection of fissile material via active interrogation. In both cases, a magnetic spectrometer momentum-analyzes charged particles (in the neutron case, alpha particles accompanying neutron generation in the D-T reaction; in the tagged photon case, post-bremsstrahlung electrons) to define kinematic properties of the relevant neutral interrogation probe particle (i.e. neutron or photon). The main conclusions of the study can be briefly summarized as follows: Neutron generator: • For the solenoidal spectrometer concept, magnetic field strengths of order 1 Tesla or greater are required to keep the transverse size of the spectrometer smaller than 1 meter. The notional magnetic spectrometer design evaluated in this feasibility study uses a 5-T magnetic field and a borehole radius of 18 cm. • The design shows a potential for 4.5 Sr tagged neutron solid angle, a factor of 4.5 larger than achievable with current API neutron-generator designs. • The potential angular resolution for such a tagged neutron beam can be less than 0.5o for modest Si-detector position resolution (3 mm). Further improvement in angular resolution can be made by using Si-detectors with better position resolution. • The report documents several features of a notional generator design incorporating the alpha-particle spectrometer concept, and outlines challenges involved in the magnetic field design. Tagged photon interrogation: • We investigated a method for discriminating fissile from benign cargo-material response to an energy-tagged photon beam. The method relies upon coincident detection of the tagged photon and a photoneutron or photofission neutron produced in the target material. The method exploits differences in the shape of the neutron production cross section as a function of incident photon energy in order to discriminate photofission yield from photoneutrons emitted by non-fissile materials. Computational tests of the interrogation method as applied to material composition assay of a simple, multi-layer target suggest that the tagged-photon information facilitates precise (order 1% thickness uncertainty) reconstruction of the constituent thicknesses of fissile (uranium) and high-Z (Pb) constituents of the test targets in a few minutes of photon-beam exposure. We assumed an 18-MeV endpoint tagged photon beam for these simulations. • The report addresses several candidate design and data analysis issues for beamline infrastructure required to produce a tagged photon beam in a notional AI-dedicated facility, including the accelerator and tagging spectrometer.

Jordan, David V.; Baciak, James E.; Stave, Sean C.; Chichester, David; Dale, Daniel; Kim, Yujong; Harmon, Frank

2012-10-16

309

A brief examination of optical tagging technologies.  

SciTech Connect

Presented within this report are the results of a brief examination of optical tagging technologies funded by the Laboratory Directed Research and Development (LDRD) program at Sandia National Laboratories. The work was performed during the summer months of 2002 with total funding of $65k. The intent of the project was to briefly examine a broad range of approaches to optical tagging concentrating on the wavelength range between ultraviolet (UV) and the short wavelength infrared (SWIR, {lambda} < 2{micro}m). Tagging approaches considered include such things as simple combinations of reflective and absorptive materials closely spaced in wavelength to give a high contrast over a short range of wavelengths, rare-earth oxides in transparent binders to produce a narrow absorption line hyperspectral tag, and fluorescing materials such as phosphors, dies and chemically precipitated particles. One technical approach examined in slightly greater detail was the use of fluorescing nano particles of metals and semiconductor materials. The idea was to embed such nano particles in an oily film or transparent paint binder. When pumped with a SWIR laser such as that produced by laser diodes at {lambda}=1.54{micro}m, the particles would fluoresce at slightly longer wavelengths, thereby giving a unique signal. While it is believed that optical tags are important for military, intelligence and even law enforcement applications, as a business area, tags do not appear to represent a high on return investment. Other government agencies frequently shop for existing or mature tag technologies but rarely are interested enough to pay for development of an untried technical approach. It was hoped that through a relatively small investment of laboratory R&D funds, enough technologies could be identified that a potential customers requirements could be met with a minimum of additional development work. Only time will tell if this proves to be correct.

Ackermann, Mark R.; Cahill, Paul A. (Aspecular Optics, Dayton, OH); Drummond, Timothy J.; Wilcoxon, Jess Patrick

2003-07-01

310

The effect of PIT tagging on survival, tag retention, and weight gain in fingerling white bass  

Technology Transfer Automated Retrieval System (TEKTRAN)

We tagged fingerling white bass Morone chrysops with Passive Integrated Transponders (PIT) at two body locations (peritoneal cavity and dorsal musculature) and six weight classes (-6, 10, 14, 19, 25, and 30 g) to evaluate survival, tag retention, and weight gain during a 28-day experimental period. ...

311

The Unbearable Lightness of Tagging A Case Study in Morphosyntactic Tagging of Polish  

E-print Network

The Unbearable Lightness of Tagging A Case Study in Morphosyntactic Tagging of Polish Adam be constructed more carefully and, in effect, should be light in at least three senses: 1) they should pay less be as- signed to light units, typically not longer than orthographic words. A tagset for Polish

Przepiórkowski, Adam

312

Flexible tag microlab development: Gas sensors integration in RFID flexible tags for food logistic  

Microsoft Academic Search

The enabling technologies for the development of a flexible tag microlab for food monitoring during the logistic chain will be presented. The realisation of the system includes the integration of physical and chemical sensors with Radio Frequency IDentification (RFID) communication capabilities. The first ISO 15693 compliant semi-active tag prototype, including low power control electronics, RFID antenna, commercial sensors, memory and

Estefania Abad; Stefano Zampolli; Santiago Marco; Andrea Scorzoni; Barbara Mazzolai; Aritz Juarros; David Gómez; Ivan Elmi; Gian Carlo Cardinali; José M. Gómez; Francisco Palacio; Michelle Cicioni; Alessio Mondini; Thomas Becker; Ilker Sayhan

2007-01-01

313

S-Tag Rapid Assay The SeTag rapid assay is based on the  

E-print Network

S-Tag Rapid Assay The SeTag rapid assay is based on the reconstitution of ribonucleolytic (RNase S to th e amount of recombinant protein present in the sample. T ypica l assay profil es are shown the conditions of the Rapid Assay. By comparing dIe absorbance profile of a known standard (provided in th e kit

Raines, Ronald T.

314

Supporting Information A dipicolinic acid tag for rigid lanthanide tagging of proteins and paramagnetic  

E-print Network

hydrochloric acid (2 x 20 ml). The organic phase was dried over calcium chloride, filtered, and the solventS1 Supporting Information A dipicolinic acid tag for rigid lanthanide tagging of proteins,6-pyridine dicarboxylic acid General Procedures Dipicolinic acid and p-toluenesulfonyl chloride were

Otting, Gottfried

315

Accurate Protein Complex Retrieval by Affinity Enrichment Mass Spectrometry (AE-MS) Rather than Affinity Purification Mass Spectrometry (AP-MS)*  

PubMed Central

Protein–protein interactions are fundamental to the understanding of biological processes. Affinity purification coupled to mass spectrometry (AP-MS) is one of the most promising methods for their investigation. Previously, complexes were purified as much as possible, frequently followed by identification of individual gel bands. However, todays mass spectrometers are highly sensitive, and powerful quantitative proteomics strategies are available to distinguish true interactors from background binders. Here we describe a high performance affinity enrichment-mass spectrometry method for investigating protein–protein interactions, in which no attempt at purifying complexes to homogeneity is made. Instead, we developed analysis methods that take advantage of specific enrichment of interactors in the context of a large amount of unspecific background binders. We perform single-step affinity enrichment of endogenously expressed GFP-tagged proteins and their interactors in budding yeast, followed by single-run, intensity-based label-free quantitative LC-MS/MS analysis. Each pull-down contains around 2000 background binders, which are reinterpreted from troubling contaminants to crucial elements in a novel data analysis strategy. First the background serves for accurate normalization. Second, interacting proteins are not identified by comparison to a single untagged control strain, but instead to the other tagged strains. Third, potential interactors are further validated by their intensity profiles across all samples. We demonstrate the power of our AE-MS method using several well-known and challenging yeast complexes of various abundances. AE-MS is not only highly efficient and robust, but also cost effective, broadly applicable, and can be performed in any laboratory with access to high-resolution mass spectrometers. PMID:25363814

Keilhauer, Eva C.; Hein, Marco Y.; Mann, Matthias

2015-01-01

316

Loop realizations of quantum affine algebras  

SciTech Connect

We give a simplified description of quantum affine algebras in their loop presentation. This description is related to Drinfeld's new realization via halves of vertex operators. We also define an idempotent version of the quantum affine algebra which is suitable for categorification.

Cautis, Sabin [Department of Mathematics, University of Southern California, Los Angeles, California, 90089 (United States); Licata, Anthony [Department of Mathematics, Australian National University, Canberra (Australia)

2012-12-15

317

Lipopolysaccharide affinity for titanium implant biomaterials  

Microsoft Academic Search

Statement of problem. Lipopolysaccharide (LPS) affinity for titanium implant biomaterials could affect crevicular LPS concentrations and thereby influence periimplant inflammation.Purpose of study. The purpose of this study was to evaluate Porphyromonas gingivalis and Escherichia coli LPS affinity for titanium biomaterials groups that differed in surface oxide composition and surface roughness.Material and method. Polished and abraded grade 1 commercially pure titanium

Steven K. Nelson; Kent L. Knoernschild; Fonda G. Robinson; George S. Schuster

1997-01-01

318

Affine Constellations Without Mutually Unbiased Counterparts  

E-print Network

It has been conjectured that a complete set of mutually unbiased bases in a space of dimension d exists if and only if there is an affine plane of order d. We introduce affine constellations and compare their existence properties with those of mutually unbiased constellations, mostly in dimension six. The observed discrepancies make a deeper relation between the two existence problems unlikely.

Stefan Weigert; Thomas Durt

2010-07-22

319

Affine\\/ Photometric Invariants for Planar Intensity Patterns  

Microsoft Academic Search

The paper contributes to the viewpoint invariant recognition of planar patterns, especially labels and signs under affine deformations. By their nature, the information of such eye-catchers is not contained in the outline or frame — they often are affinely equivalent like parallelograms and ellipses — but in the intensity content within. Moment invariants are well suited for their recognition. They

Luc J. Van Gool; Theo Moons; Dorin Ungureanu

1996-01-01

320

Recursive Turtle Programs and Iterated Affine Transformations  

E-print Network

Recursive Turtle Programs and Iterated Affine Transformations Tao Ju*, Scott Schaefer, Ron Goldman of equivalence between the class of fractals created by Recursive Turtle Programs (RTP) and Iterated Affine, turtle graphics 1 Introduction In computer graphics, there are several different methods for generating 2

Schaefer, Scott

321

AffinDB: a freely accessible database of affinities for protein-ligand complexes from the PDB  

Microsoft Academic Search

AffinDB is a database of affinity data for structurally resolved protein-ligand complexes from the Protein Data Bank (PDB). It is freely accessible at http:\\/\\/www. agklebe.de\\/affinity. Affinity data are collected from the scientific literature, both from primary sources describing the original experimental work of affinity determination and from secondary references which report affinity values determined by others. AffinDB currently contains over

Peter Block; Christoph A. Sotriffer; Ingo Dramburg; Gerhard Klebe

2006-01-01

322

Improving image segmentation by learning region affinities  

SciTech Connect

We utilize the context information of other regions in hierarchical image segmentation to learn new regions affinities. It is well known that a single choice of quantization of an image space is highly unlikely to be a common optimal quantization level for all categories. Each level of quantization has its own benefits. Therefore, we utilize the hierarchical information among different quantizations as well as spatial proximity of their regions. The proposed affinity learning takes into account higher order relations among image regions, both local and long range relations, making it robust to instabilities and errors of the original, pairwise region affinities. Once the learnt affinities are obtained, we use a standard image segmentation algorithm to get the final segmentation. Moreover, the learnt affinities can be naturally unutilized in interactive segmentation. Experimental results on Berkeley Segmentation Dataset and MSRC Object Recognition Dataset are comparable and in some aspects better than the state-of-art methods.

Prasad, Lakshman [Los Alamos National Laboratory; Yang, Xingwei [TEMPLE UNIV.; Latecki, Longin J [TEMPLE UNIV.

2010-11-03

323

Electronic tagging and integrated product intelligence  

NASA Astrophysics Data System (ADS)

The advent of 'intelligent,' electronic data bearing tags is set to revolutionize the way industrial and retail products are identified and tracked throughout their life cycles. The dominant system for unique identification today is the bar code, which is based on printed symbology and regulated by the International Article Numbering Association. Bar codes provide users with significant operational advantages and generate considerable added value to packaging companies, product manufacturers, distributors and retailers, across supply chains in many different sectors, from retailing, to baggage handling and industrial components, e.g., for vehicles or aircraft. Electronic tags offer the potential to: (1) record and store more complex data about the product or any modifications which occur during its life cycle; (2) access (and up-date) stored data in real time in a way which does not involve contact with the product or article; (3) overcome the limitations imposed by systems which rely on line-of-sight access to stored data. Companies are now beginning to consider how electronic data tags can be used, not only to improve the efficiency of their supply chain processes, but also to revolutionize the way they do business. This paper reviews the applications and business opportunities for electronic tags and outlines CEST's strategy for achieving an 'open' standard which will ensure that tags from different vendors can co-exist on an international basis.

Swerdlow, Martin; Weeks, Brian

1996-03-01

324

Ultrafast tissue staining with chemical tags.  

PubMed

Genetically encoded fluorescent proteins and immunostaining are widely used to detect cellular and subcellular structures in fixed biological samples. However, for thick or whole-mount tissue, each approach suffers from limitations, including limited spectral flexibility and lower signal or slow speed, poor penetration, and high background labeling, respectively. We have overcome these limitations by using transgenically expressed chemical tags for rapid, even, high-signal and low-background labeling of thick biological tissues. We first construct a platform of widely applicable transgenic Drosophila reporter lines, demonstrating that chemical labeling can accelerate staining of whole-mount fly brains by a factor of 100. Using viral vectors to deliver chemical tags into the mouse brain, we then demonstrate that this labeling strategy works well in mice. Thus this tag-based approach drastically improves the speed and specificity of labeling genetically marked cells in intact and/or thick biological samples. PMID:25157152

Kohl, Johannes; Ng, Julian; Cachero, Sebastian; Ciabatti, Ernesto; Dolan, Michael-John; Sutcliffe, Ben; Tozer, Adam; Ruehle, Sabine; Krueger, Daniel; Frechter, Shahar; Branco, Tiago; Tripodi, Marco; Jefferis, Gregory S X E

2014-09-01

325

Engineered mammalian vector to express EGFP-tagged proteins as biomarkers.  

PubMed

Due to its specialized post-translational machinery, mammalian cells represent an interesting and not fully explored system to express snake toxins. Therefore, in this work, we built up a new mammalian expression vector that enhances the feasibility to use mammalian cells to express proteins as biomarkers. Among the modifications, an Ig? signal peptide and a 6xHis tag were inserted into this vector in order to drive the protein to the supernatant and simplify its purification, respectively. In addition, to facilitate selection of high producing clones and also tag proteins which may function as a biomarker, the sequence of enhanced green fluorescent protein (EGFP) was added. The efficiency of the resulting vector (pToxEGFP) was tested by cloning and expressing the viper venom disintegrin echistatin (Ech) that due to its affinity to integrin ?v?3 was tested as a molecular marker. Expression of EGFP-Ech was achieved in CHO-DXB11 cells resulting in a yield of 22 mg/L. The binding activity of this chimera protein was successfully achieved on human umbilical vein endothelial cells which highly express ?v?3. The results indicate that pToxEGFP may constitute an efficient and versatile expression vector to express tagged proteins with potential biomarker activity. PMID:21847674

Magalhães, Geraldo Santana; Novo, Juliana Branco; Clissa, Patricia Bianca; Della Casa, Maisa Splendore; Butera, Diego; da Silva, Ana Maria Moura

2012-06-01

326

Tags to Track Illicit Uranium and Plutonium  

SciTech Connect

With the expansion of nuclear power, it is essential to avoid diversion of nuclear materials into the hands of 'rogue nations,' terrorists, and other opportunists. This paper describes (1) the use of a detection tag to make it easier to detect smuggled material by creating a nuclear fingerprint and (2) the use of attribution tags to enable law enforcement to determine where any recovered stolen nuclear materials came from, identify the individuals responsible for the unlawful diversion, and reduce future loss of nuclear materials.

Haire, Marvin Jonathan [ORNL

2007-01-01

327

B mixing and flavor tagging at CDF  

SciTech Connect

The CDF Collaboration has made a preliminary measurement of B{sub d} mixing as a first step toward measuring mixing in the B{sub s} system. Flavor tagging using opposite-side jets and muons as well as same-side tagging schemes have been applied. Results agree well with precise results from the B-factories. They use these results to estimate CDF's B{sub s} mixing range using the present data set ({approx} 250 pb{sup -1}) and extrapolate to the potential from larger data sets in future running.

Russ, James S.; /Carnegie Mellon U.

2004-12-01

328

Identifying Nuclear Materials Using Tagged Muons  

E-print Network

Experimental results from a new technique that uses neutrons generated by stopped cosmic-ray muons to identify nuclear materials are described. The neutrons are used to tag muon-induced fission events in actinides and laminography is used to form images of the stopping material. This technique allows the imaging of uranium objects tagged using muon tracking detectors located above or to the side of the objects. The specificity of the technique to significant quantities of nuclear material along with its insensitivity to spatial details may provide a new method for the task of warhead verification for future arms reduction treaties.

C. L. Morris; J. D. Bacon; K. Borodzin; J. M. Durham; J. M. Fabritius II; E. Guardincerri; A. Hecht; E. C. Milner; H. Miyadera; J. O. Perry; D. Poulson

2014-06-04

329

A Study on Exploring People’s Affinity for Solitude  

E-print Network

This study sought to investigate solitude as a phenomenon. People’s affinity for solitude and the antecedents of affinity for solitude were of crucial interest to the study because affinity for solitude has been considered a strong determinant...

Lee, Sunwoo

2013-01-09

330

Polymer versus monomer as displacer in immobilized metal affinity chromatography.  

PubMed

Successful immobilized metal affinity chromatography (IMAC) of proteins on Cu2+-iminodiacetic acid Sepharose has been carried out in a displacement mode using a synthetic copolymer of vinyl imidazole and vinyl caprolactam [poly(VI-VCL)] as a displacer. Vinyl caprolactam renders the co-polymer with the thermosensitivity, e.g., property of the co-polymer to precipitate nearly quantitatively from aqueous solution on increase of the temperature to 48 degrees C. A thermostable lactate dehydrogenase from the thermophilic bacterium Bacillus stearothermophilus modified with a (His)6-tag [(His)6-LDH] has been purified using an IMAC column. For the first time it was clearly demonstrated that a polymeric displacer [poly(VI-VCL)] was more efficient compared to a monomeric displacer (imidazole) of the same chemical nature, probably due to the multipoint interaction of imidazole groups within the same macromolecule with one Cu2+ ion. Complete elution of bound (His)6-LDH has been achieved at 3.7 mM concentration of imidazole units of the co-polymer (5 mg/ml), while this concentration of free imidazole was sufficient to elute only weakly bound proteins. Complete elution of (His)6-LDH by the free imidazole was achieved only at concentrations as high as 160 mM. Thus, it was clearly demonstrated, that the efficiency of low-molecular-mass displacer could be improved significantly by converting it into a polymeric displacer having interacting groups of the same chemical nature. PMID:11334341

Arvidsson, P; Ivanov, A E; Galaev IYu; Mattiasson, B

2001-04-01

331

Low-cost electromagnetic tagging : design and implementation  

E-print Network

Several implementations of chipless RFID (Radio Frequency Identification) tags are presented and discussed as low-cost alternatives to chip-based RFID tags and sensors. An overview of present-day near-field electromagnetic ...

Fletcher, Richard R. (Richard Ribon)

2002-01-01

332

OpenTag : privacy control methods in RFID  

E-print Network

The work documented in this thesis is part of the OpenTag project, which has the goal of designing and developing a flexible and more powerful RFID system to meet the needs of the approaching ubiquitous tagging of everyday ...

Shen, Daniel Z

2006-01-01

333

VISUALLY SUMMARIZING THE EVOLUTION OF DOCUMENTS UNDER A SOCIAL TAG  

E-print Network

. Additionally, we show the effectiveness by adding alien resources under a tag. Our approach indeed visualizes are capable to deduce the meanings of resources from tags. In real life, often both assumptions are vi- olated

Hinneburg, Alexander

334

Automated Data Tagging in the HLA  

NASA Astrophysics Data System (ADS)

One of the more powerful and popular forms of data organization implemented in most popular information sharing web applications is data tagging. With a rich user base from which to gather and digest tags, many interesting and often unanticipated yet very useful associations are revealed. With regard to an existing information, the astronomical community has a rich pool of existing digitally stored and searchable data than any of the currently popular web community, such as You Tube or My Space, had when they started. In initial experiments with the search engine for the Hubble Legacy Archive, we have created a simple yet powerful scheme by which the information from a footprint service, the NED and SIMBAD catalog services, and the ADS abstracts and keywords can be used to initially tag data with standard keywords. By then ingesting this into a public ally available information search engine, such as Apache Lucene, one can create a simple and powerful data tag search engine and association system. By then augmenting this with user provided keys and usage pattern analysis, one can produce a powerful modern data mining system for any astronomical data warehouse.

Gaffney, N. I.; Miller, W. W.

2008-08-01

335

Measurement Protocols for Optimized Fuel Assembly Tags  

SciTech Connect

This report describes the measurement protocols for optimized tags that can be applied to standard fuel assemblies used in light water reactors. This report describes work performed by the authors at Pacific Northwest National Laboratory for NA-22 as part of research to identify specific signatures that can be developed to support counter-proliferation technologies.

Gerlach, David C.; Mitchell, Mark R.; Reid, Bruce D.; Gesh, Christopher J.; Hurley, David E.

2008-11-01

336

Tag SNP selection using particle swarm optimization.  

PubMed

Single nucleotide polymorphisms (SNPs) are the most abundant form of genetic variations amongst species. With the genome-wide SNP discovery, many genome-wide association studies are likely to identify multiple genetic variants that are associated with complex diseases. However, genotyping all existing SNPs for a large number of samples is still challenging even though SNP arrays have been developed to facilitate the task. Therefore, it is essential to select only informative SNPs representing the original SNP distributions in the genome (tag SNP selection) for genome-wide association studies. These SNPs are usually chosen from haplotypes and called haplotype tag SNPs (htSNPs). Accordingly, the scale and cost of genotyping are expected to be largely reduced. We introduce binary particle swarm optimization (BPSO) with local search capability to improve the prediction accuracy of STAMPA. The proposed method does not rely on block partitioning of the genomic region, and consistently identified tag SNPs with higher prediction accuracy than either STAMPA or SVM/STSA. We compared the prediction accuracy and time complexity of BPSO to STAMPA and an SVM-based (SVM/STSA) method using publicly available data sets. For STAMPA and SVM/STSA, BPSO effective improved prediction accuracy for smaller and larger scale data sets. These results demonstrate that the BPSO method selects tag SNP with higher accuracy no matter the scale of data sets is used. PMID:20039435

Chuang, Li-Yeh; Yang, Cheng-San; Ho, Chang-Hsuan; Yang, Cheng-Hong

2010-01-01

337

Fish Tagging Forum February 9, 2012  

E-print Network

or modified during the meeting are available under the "Past Meeting ­ Feb 2012" section at http. 2. Discussion of Genetic Marking Matt Campbell (IDFG) and Shawn Narum (CRITFC) provided an overview of genetic marking with an emphasis on Parental Base Tagging (PBT) and Genetic Stock Identification (GSI

338

RFID-Tags for Anti-counterfeiting  

Microsoft Academic Search

RFID-tags are becoming very popular tools for identiflcation of products. As they have a small microchip on board, they ofier func- tionality that can be used for security purposes. This chip functionality makes it possible to verify the authenticity of a product and hence to detect and prevent counterfeiting. In order to be successful for these secu- rity purposes too,

Pim Tuyls; Lejla Batina

2006-01-01

339

Untraceable RFID tags via insubvertible encryption  

Microsoft Academic Search

We introduce a new cryptographic primitive, called insubvertible encryption, that produces ciphertexts which can be randomized without the need of any key material. Unlike plain universal re-encryption schemes, insubvertible encryption prevents against adversarial exploitation of hidden channels, by including certificates proving that the ciphertext can only be decrypted by authorized parties.The scheme can be applied to RFID tags, providing strong

Giuseppe Ateniese; Jan Camenisch; Breno de Medeiros

2005-01-01

340

TagSense RFID Communications Mikyle Bengtson  

E-print Network

TagSense RFID Communications Mikyle Bengtson Faculty Mentor: Professor Russell Tessier This project focuses on the communications between an RFID reader and several RFID transmitters. Once complete so that response times in mass-casualty incidents can be reduced. At the heart of this system is RFID

Mountziaris, T. J.

341

EFFECTS OF TAGGING ON RED SALMON  

E-print Network

, and length from the mouth to a beaver dam that formed an impassable barrier was two and one- eighth miles completion of tagging, two men started up the stream on survey. They covered all of the area up to the beaver

342

Lemmatisation as a Tagging Task Andrea Gesmundo  

E-print Network

our approach on eight languages reaching a new state-of-the-art level for the lemmatisation task. 1 Introduction Lemmatisation and part-of-speech (POS) tagging are necessary steps in automatic processing of lan- tomatic processing such as information retrieval, as well as for using language corpora in linguistic re

Genève, Université de

343

A TECHNIQUE FOR TAGGING DEEPWATER FISH  

E-print Network

, Phillips (1968) attempted to mark California rockfish by using detachable hooks with "Peterson type on commerciallongline fishing gear (see Free- man and Turner 1977 for a description of the gear). These tags consisted and effortlogs) and also because of the localized nature ofthe tilefish ports (Le., only two ports landed

344

Page 1 of 1 Fish Tagging Forum  

E-print Network

information needs. LUNCH 1:00 to 2:00 Scenario Analysis ­ Genetic Marks (Kevin Kytola) Using the Management the theoretical consequences of not investing in the application of genetic marking. 2:00 to 4:00 Scenario Analysis ­ Coded Wire Tags (Kevin Kytola) Using the Management Question and Indicator Network Diagram

345

Page 1 of 1 Fish Tagging Forum  

E-print Network

to the F&W Committee and Council. 9:30 to 12:00 Discussion of Genetic Marking Subject Matter Experts will discuss the use of genetic marking technologies to produce data that support answering management for discussion and modification by the group. 3:45 to 4:00 Review Tagging Program Cost Information Rick

346

A Theme Landscape for Tagged Data  

Microsoft Academic Search

The wide variety of visualization methods for numerical values have, up until now, outweighed the relatively modest selection available for nominal dimensions. Theme Landscape is a visualization application designed for tagged data that typically contains a large number of nominal dimensions. It provides an overview of large data volumes, doing so by positioning objects in a landscape according to how

Evelyn Munster

2010-01-01

347

Fish Tagging Forum February 12, 2013  

E-print Network

the Program as well as other issues discussed in the ISAB/ISRP report 3 #12;FTF Timeline and Process Questions and Indicator Worksheets Work Products · Indicator & Tag Relationship Diagrams (aka Spider Chart Analysis · IEAB Cost Modeling Work Products (TBD) · Gaps · Overlaps · Process Efficiencies · Policy Choices

348

Fish Tagging Forum February 12, 2013  

E-print Network

efforts that take place under the Council's Fish and Wildlife Program, including expense, capital and reimbursable programs. · address the cost effectiveness and the program effectiveness of tagging under;The Whole Enchilada.... 12 1 E Fish out Harvest Hydro 5A Age one recruitment for sturgeon Hatchery 1A

349

Imaging mass spectrometer with mass tags  

DOEpatents

A method of analyzing biological material by exposing the biological material to a recognition element, that is coupled to a mass tag element, directing an ion beam of a mass spectrometer to the biological material, interrogating at least one region of interest area from the biological material and producing data, and distributing the data in plots.

Felton, James S.; Wu, Kuang Jen J.; Knize, Mark G.; Kulp, Kristen S.; Gray, Joe W.

2013-01-29

350

Imaging mass spectrometer with mass tags  

DOEpatents

A method of analyzing biological material by exposing the biological material to a recognition element, that is coupled to a mass tag element, directing an ion beam of a mass spectrometer to the biological material, interrogating at least one region of interest area from the biological material and producing data, and distributing the data in plots.

Felton, James S.; Wu, Kuang Jen; Knize, Mark G.; Kulp, Kristen S.; Gray, Joe W.

2010-06-01

351

Closeup of Solar Tadpoles with time tags  

NSDL National Science Digital Library

Here is a close-up view of dark tentacles or tadpoles moving towards the solar surface in this solar flare of April 21, 2002 seen by TRACE. One theory proposed in this press release is that they are due to voids created by magnetic reconnection in the flare. This version of the visualization displays the instrument clock time tags.

Tom Bridgman

2003-04-11

352

Closeup of Solar Tadpoles without time tags  

NSDL National Science Digital Library

Here is a close-up view of dark tentacles or tadpoles moving towards the solar surface in this solar flare of April 21, 2002 seen by TRACE. One theory proposed in this press release is that they are due to voids created by magnetic reconnection in the flare. This version of the visualization does not display the instrument clock time tags.

Tom Bridgman

2003-04-11

353

Efficient Object Identification with Passive RFID Tags  

E-print Network

identification (RFID) promises to be an unobtrusive, practical, cheap, yet flexible technology for identificationEfficient Object Identification with Passive RFID Tags Harald Vogt Department of Computer Science quantities of inexpensive goods. The -chip by Hitachi [14] is an example of a tiny RFID chip that can

354

COMPACT WIDEBAND TAG ANTENNA FOR UHF RFID  

E-print Network

. To get a physical operation of the proposed antenna, a simplified structure is presented and analyzed of the proposed tag antenna, we analyze and present an equivalent circuit model with a simplified structure structure and operation principle of the proposed antenna. Even though the structure does not have meander

Myung, Noh-Hoon

355

University of Toronto Safe to Remove Tag  

E-print Network

University of Toronto Safe to Remove Tag Where necessary, the equipment identified below has been. Equipment Department, Building Name, and Room Number To the best of my knowledge, this equipment or location of T Laboratory Hazardous Waste Management and Disposal Manual http

Sokolowski, Marla

356

Multiple Affinity Removal System Another Breakthrough  

E-print Network

or Mouse Serum High Abundant Proteins (Human: Albumin, lgG, IgA, Transferrin, Haptoglobin, Antitrypsin; Mouse: Albumin, lgG, Transferrin) Multiple Affinity Removal Column Low-Abundant Proteins (Biomarkers

Lebendiker, Mario

357

Hylleraas hydride binding energy: diatomic electron affinities.  

PubMed

Theoretical adiabatic electron affinities are often considered inaccurate because they are referenced to only a single value. Ground state electron affinities for all the main group elements and homonuclear diatomics were identified recently using the normalized binding energy of the hydrogen atom: [0.75420375(3)/2?=?0.37710187(1) eV]. Here we revisit experimental values and extend the identifications to diatomics in the G2-1 set. We assign new ground state electron affinities: (eV) Cl2, 3.2(2); Br2, 2.87(14); CH, 2.1(2); H2, 0.6 ; NH, 1.1, SiH, 1.90. Anion Morse potentials are calculated for H2 and N2 from positive electron affinities and for hyperfine superoxide states for the first time. PMID:25758340

Chen, Edward S; Keith, Herman; Lim, Tristan; Pham, Dang; Rosenthal, Reece; Herder, Charles; Pai, Sunil; Flores, R A; Chen, Edward C M

2015-04-01

358

Combinatorics in affine flag varieties James Parkinson  

E-print Network

Combinatorics in affine flag varieties James Parkinson Institut f¨ur Mathematische Strukturtheorie Technische Universit¨at Graz Steyrergasse 30/III, A-8010 Graz Austria parkinson@weyl.math.tu-graz.ac.at Arun

Ram, Arun

359

PRINCIPLES OF AFFINITY-BASED BIOSENSORS  

EPA Science Inventory

Despite the amount of resources that have been invested by national and international academic, government, and commercial sectors to develop affinity-based biosensor products, little obvious success has been realized through commercialization of these devices for specific applic...

360

Protein purification using PDZ affinity chromatography.  

PubMed

PDZ domains function in nature as protein-binding domains within scaffold and membrane-associated proteins. They comprise approximately 90 residues and undergo specific, high-affinity interactions with complementary C-terminal peptide sequences, other PDZ domains, and/or phospholipids. We have previously shown that the specific, strong interactions of PDZ domains with their ligands make them well suited for use in affinity chromatography. This unit provides protocols for the PDZ affinity chromatography procedure that are applicable for the purification of proteins that contain PDZ domains or PDZ domain-binding ligands, either naturally or introduced by genetic engineering. We detail the preparation of affinity resins composed of PDZ domains or PDZ domain peptide ligands coupled to solid supports. These resins can be used to purify proteins containing endogenous or genetically introduced PDZ domains or ligands, eluting the proteins with free PDZ domain peptide ligands. © 2015 by John Wiley & Sons, Inc. PMID:25829303

Walkup, Ward G; Kennedy, Mary B

2015-01-01

361

Power Margin Reduction in Linear passive UHF RFID tag arrays  

E-print Network

communication with the tag antenna gathering energy from the electromagnetic wave transmitted by reader, and then using the energy to power a microchip. This then changes the load on the antenna to achieve backscatter modulation and communication back... frequency. Tag detuning can be caused by the changes in the antenna impedance when the tag is placed on an object or when other objects are present in the vicinity of the tag due to their mutual inductances [10]. The voltage induced in the antenna...

Zhang, Qi; Crisp, Michael; White, Ian H.; Penty, Richard V.

2014-07-17

362

Proton affinities of saturated aliphatic methyl esters  

Microsoft Academic Search

The kinetic method was used to determine the proton affinities of methyl esters of several saturated fatty acids. Decompositions\\u000a of the proton-bound dimers of the methyl esters, AHB+, were observed under different conditions with two instruments. The proton affinities (PAs) of the methyl esters increase\\u000a continually with increasing carbon number in the acid. Equilibrium and initial rate experiments were performed

Jason Evans; Gordon Nicol; Burnaby Munson

2000-01-01

363

Mepanipyrim haptens and antibodies with nanomolar affinity.  

PubMed

Mepanipyrim is an anilinopyrimidine fungicide used worldwide for crop protection. With the aim of developing useful immunoreagents for mepanipyrim immunoanalysis, two new functionalized derivatives were prepared and antibodies were generated. Affinity and specificity were assessed by direct and indirect competitive ELISA using homologous and heterologous conjugates. Although all antibodies were selective for the target analyte, the immunizing hapten structure was revealed as a determinant for high-affinity antibody production (IC(50) = 3 nM). PMID:23666476

Esteve-Turrillas, Francesc A; Mercader, Josep V; Agulló, Consuelo; Abad-Somovilla, Antonio; Abad-Fuentes, Antonio

2013-06-21

364

Affinity Electrophoresis Using Ligands Attached To Polymers  

NASA Technical Reports Server (NTRS)

In new technique, reduction of electrophoretic mobilities by addition of polyethylene glycol to ligands increases electrophoretic separabilities. In immuno-affinity electrophoresis, modification of ligands extends specificity of electrophoretic separation to particles having surface electric-charge structures otherwise making them electrophoretically inseparable. Modification of antibodies by polyethylene glycol greatly reduces ability to aggregate while enhancing ability to affect electrophoretic mobilities of cells. In hydrophobic-affinity electrophoresis, addition of polyethylene glycol reduces tendency toward aggregation of cells or macromolecules.

Van Alstine, James M.; Snyder, Robert S.; Harris, J. M.; Brooks, D. E.

1990-01-01

365

The primal power affine scaling method  

Microsoft Academic Search

In this paper, we present a variant of the primal affine scaling method, which we call the primal power affine scaling method. This method is defined by choosing a realr>0.5, and is similar to the power barrier variant of the primal-dual homotopy methods considered by den Hertog, Roos and Terlaky and Sheu and Fang. Here, we analyze the methods forr>1.

Romesh Saigal

1996-01-01

366

Measuring Social Tag Confidence: Is It a Good or Bad Tag?  

Microsoft Academic Search

\\u000a Social tagging is an increasingly popular way to describe latent semantic information of web resources and thus is widely\\u000a used to improve the performance of information retrieval system. However, there also has been significant variance of the\\u000a quality of social tags because they can be annotated by folks on the web freely. As a consequence, how to measure the quality

Xiwu Gu; Xianbing Wang; Ruixuan Li; Kunmei Wen; Yufei Yang; Weijun Xiao

367

77 FR 51761 - Proposed Information Collection; Comment Request; Groundfish Tagging Program  

Federal Register 2010, 2011, 2012, 2013, 2014

...Alaska), California, Oregon, and Washington. Fish movement information from recovered tags is used in population dynamics models for stock assessment. There are two general categories of tags. Simple plastic tags (spaghetti tags) are...

2012-08-27

368

Guaranteed Pre{Tagging for the Brill Tagger Saif Mohammad and Ted Pedersen  

E-print Network

neighbors. Pre{tagging can be thought of as the process of manually priming or seeding the tagging process, and is intended to help improve the accuracy of tagging by providing a reliable anchor or seed around which to tag

Pedersen, Ted

369

A SEMANTIC SPACE FOR MUSIC DERIVED FROM SOCIAL TAGS  

Microsoft Academic Search

In this paper we investigate social tags as a novel high- volume source of semantic metadata for music, using tech- niques from the fields of information retrieval and multi- variate data analysis. We show that, despite the ad hoc and informal language of tagging, tags define a low-dimensional semantic space that is extremely well-behaved at the track level, in particular

Mark Sandler

2007-01-01

370

The Searching Effectiveness of Social Tagging in Museum Websites  

ERIC Educational Resources Information Center

This paper explores the search effectiveness of social tagging which allows the public to freely tag resources, denoted as keywords, with any words as well as to share personal opinions on those resources. Social tagging potentially helps users to organize, manage, and retrieve resources. Efficient retrieval can help users put more of their focus…

Cho, Chung-Wen; Yeh, Ting-Kuang; Cheng, Shu-Wen; Chang, Chun-Yen

2012-01-01

371

Towards Tag Antenna Based Sensing An RFID Displacement Sensor  

E-print Network

Towards Tag Antenna Based Sensing ­ An RFID Displacement Sensor Rahul Bhattacharyya, Christian and cost effective. In this paper, we examine a technique to utilize a UHF RFID tag antenna as a displacement sensor by mapping structural deformation to a change in RFID tag characteristics. We evaluate how

Entekhabi, Dara

372

Optical Localization of Passive UHF RFID Tags with Integrated LEDs  

E-print Network

Optical Localization of Passive UHF RFID Tags with Integrated LEDs Alanson P. Sample, Craig--The ability to accurately localize passive UHF RFID tags in uncontrolled and unstructured environments passive UHF RFID tags with LEDs, using the Wireless Identification and Sensing Platform (WISP

Hochberg, Michael

373

Efficient Tag Detection in RFID Systems Bogdan Carbunar  

E-print Network

-sized wireless readers [4] compatible with Crossbow [5] sensors, Bluetooth enabled readers [6], SD card reader detecting RFID tags in the presence of reader interference (reader collision avoidance problem); (ii) eliminating redundant tag reports by multiple readers (optimal tag reporting problem); and (iii) minimizing

Carbunar, Bogdan

374

Social Tagging in Community Memories Luc Steels1,2  

E-print Network

and a representation of them- selves. 1 Collective Intelligence and Commu- nity Memories Social tagging burst for the semantic web. Social tagging has so far been been used for a class of appli- cations that is generallySocial Tagging in Community Memories Luc Steels1,2 1 AI Lab, Vrije Universiteit Brussel Eugenio

TAGora project

375

Electronic tagging and population structure of Atlantic bluefin tuna  

Microsoft Academic Search

Electronic tags that archive or transmit stored data to satellites have advanced the mapping of habitats used by highly migratory fish in pelagic ecosystems. Here we report on the electronic tagging of 772 Atlantic bluefin tuna in the western Atlantic Ocean in an effort to identify population structure. Reporting electronic tags provided accurate location data that show the extensive migrations

Barbara A. Block; Steven L. H. Teo; Andreas Walli; Andre Boustany; Michael J. W. Stokesbury; Charles J. Farwell; Kevin C. Weng; Heidi Dewar; Thomas D. Williams

2005-01-01

376

CS229 Project Report: Automated photo tagging in Facebook  

E-print Network

CS229 Project Report: Automated photo tagging in Facebook Sebastian Schuon, Harry Robertson, Hao identifying and tagging users in photos on a social networking environment known as Facebook. The presented au- tomatic facial tagging system is split into three subsys- tems: obtaining image data from Facebook

Pratt, Vaughan

377

Doctoral Thesis A Study on Cryptographic Protocols for RFID Tags  

E-print Network

Doctoral Thesis RFID A Study on Cryptographic Protocols for RFID Tags ( Dang, Nguyen Duc 2010 #12;RFID A Study on Cryptographic Protocols for RFID Tags #12;A Study on Cryptographic Protocols for RFID Tags Advisor : Professor Kim, Kwangjo by Dang, Nguyen Duc Department of Information

Kim, Kwangjo

378

How Spatial Segmentation improves the Multimodal Geo-Tagging  

E-print Network

How Spatial Segmentation improves the Multimodal Geo-Tagging Pascal Kelm Communication Systems are tagged with the geo-information of the most similar training image within the regions that is previously for geo- tag prediction designed to exploit the relative advantages of textual and visual modalities. We

Wichmann, Felix

379

Accurate Visual Features for Automatic Tag Correction in Videos  

E-print Network

Accurate Visual Features for Automatic Tag Correction in Videos Hoang-Tung Tran, Elisa Fromont-Etienne, Fr Abstract. We present a new system for video auto tagging which aims at correcting the tags provided by users for videos uploaded on the In- ternet. Unlike most existing systems, in our proposal, we

Paris-Sud XI, Université de

380

Tag Interactions in MultiAgent Systems: Environment Support  

Microsoft Academic Search

Abstract. Tag interactions refer to interactions in which software agents are intentionally or opportunistically involved. Intentional tag interactions allow agents to observe each other, like when one observes the physical condition of others. Opportunistic tag interactions occur when one agent receives information about others without requesting for it, like when,one realizes that a friend is ill. Such cases seem natural

Eric Platon; Nicolas Sabouret; Shinichi Honiden

2005-01-01

381

A model-based approach to selection of tag SNPs  

Microsoft Academic Search

BACKGROUND: Single Nucleotide Polymorphisms (SNPs) are the most common type of polymorphisms found in the human genome. Effective genetic association studies require the identification of sets of tag SNPs that capture as much haplotype information as possible. Tag SNP selection is analogous to the problem of data compression in information theory. According to Shannon's framework, the optimal tag set maximizes

Pierre Nicolas; Fengzhu Sun; Lei M. Li

2006-01-01

382

Part of Speech Tagging in Bengali Using Support Vector Machine  

Microsoft Academic Search

Part of speech (POS) tagging is the task of labeling each word in a sentence with its appropriate syntactic category called part of speech. POS tagging is a very important preprocessing task for language processing activities. This paper reports about task of POS tagging for Bengali using support vector machine (SVM). The POS tagger has been developed using a tagset

Asif Ekbal; Sivaji Bandyopadhyay

2008-01-01

383

Towards a Bootstrapping Framework for Corpus Semantic Tagging  

Microsoft Academic Search

Availability of source information for se- mantic tagging (or disambignating) words in corpora is problematic. A framework to produce a semantically tagged corpus in a domain specific perspective using as source a general purpose taxonomy (i.e. Word- Net) is here proposed. The tag set is de- rived from higher level Wordnet synsets. A methodology aiming to support semantic bootstrapping in

Roberto Basili; Michelangelo Della Rocca; Maria Teresa Pazienza

1997-01-01

384

Tagging experiments are becom-ing increasingly important in large  

E-print Network

-recovery" data is often used in the literature). The first approach, generally referred to as a Brownie model (Brownie et al., 1985), uses tag- recapture data from multiple years of tagging to provide annual estimates. The standard Brownie model is formulated in terms of rates of survival and tag recovery, but can also

385

The ACAMRIT1 semantic tagging system: progress report.  

E-print Network

words, and provide a statistical analysis of the resulting tag frequency profile. The project intends profiles of semantic tags which highlight statistically significant items for further investigation, perhaps by concordance. The 2 (Chi-squared) test is used to give a value to the words or tag frequencies

Rayson, Paul

386

A rule-based RFID tag system using ubiquitous chips  

Microsoft Academic Search

Because of the recent development of radio frequency identification (RFID) technologies, various systems for RFID tags have been proposed. Since RFID tags only have a simple function, i.e., sending data, they can be available for various purposes. Accordingly, by customizing RFID tag systems, we can expand their applications. However, previous systems have been usually proposed for one special purpose only.

Tomoki Yoshihisa; Yasue Kishino; Tsutomu Terada; Masahiko Tsukamoto; Ryohei Sagara; Teruki Sukenari; Daigo Taguchi; Shojiro Nishio

2005-01-01

387

Synaptic Tagging, Evaluation of Memories, and the Distal Reward Problem  

ERIC Educational Resources Information Center

Long-term synaptic plasticity exhibits distinct phases. The synaptic tagging hypothesis suggests an early phase in which synapses are prepared, or "tagged," for protein capture, and a late phase in which those proteins are integrated into the synapses to achieve memory consolidation. The synapse specificity of the tags is consistent with…

Papper, Marc; Kempter, Richard; Leibold, Christian

2011-01-01

388

Crystal packing of the c(6)-type cytochrome OmcF from Geobacter sulfurreducens is mediated by an N-terminal Strep-tag II.  

PubMed

The putative outer membrane c-type cytochrome OmcF from Geobacter sulfurreducens contains a single haem group and shows homology to soluble cytochromes c(6), a class of electron-transfer proteins that are typically found in cyanobacterial photosynthetic electron-transfer chains. OmcF was overexpressed heterologously in Escherichia coli as an N-terminal Strep-tag II fusion protein and isolated using streptactin-affinity chromatography followed by size-exclusion chromatography. The structure was solved by Fe SAD using data collected to a resolution of 1.86 A on a rotating copper-anode X-ray generator. In the crystal, packing interactions in one dimension were exclusively mediated through the Strep-tag II sequence. The tag and linker regions were in contact with three further monomers of OmcF, leading to a well defined electron-density map for this engineered and secondary-structure-free region of the molecule. PMID:18703839

Lukat, Peer; Hoffmann, Maren; Einsle, Oliver

2008-09-01

389

Tail proteins of phage T5: Investigation of the effect of the His6-tag position, from expression to crystallisation.  

PubMed

Upon binding to its bacterial host receptor, the tail tip of phage T5 perforates, by an unknown mechanism, the heavily armoured cell wall of the host. This allows the injection of phage DNA into the cytoplasm to hijack the cell machinery and enable the production of new virions. In the perspective of a structural study of the phage tail, we have systematically overproduced eight of the eleven T5 tail proteins, with or without a N- or a C-terminal His6-tag. The widely used Hi6-tag is very convenient to purify recombinant proteins using immobilised-metal affinity chromatography. The presence of a tag however is not always innocuous. We combined automated gene cloning and expression tests to rapidly identify the most promising constructs for proteins of phage T5 tail, and performed biochemical and biophysical characterisation and crystallisation screening on available proteins. Automated small-scale purification was adapted for two highly expressed proteins. We obtained structural information for three of the proteins. We showed that the presence of a His6-tag can have drastic effect on protein expression, solubility, oligomerisation propensity and crystal quality. PMID:25676818

Noirclerc-Savoye, Marjolaine; Flayhan, Ali; Pereira, Cindy; Gallet, Benoit; Gans, Pierre; Ebel, Christine; Breyton, Cécile

2015-05-01

390

Highly efficient purification of protein complexes from mammalian cells using a novel streptavidin-binding peptide and hexahistidine tandem tag system: Application to Bruton's tyrosine kinase  

PubMed Central

Tandem affinity purification (TAP) is a generic approach for the purification of protein complexes. The key advantage of TAP is the engineering of dual affinity tags that, when attached to the protein of interest, allow purification of the target protein along with its binding partners through two consecutive purification steps. The tandem tag used in the original method consists of two IgG-binding units of protein A from Staphylococcus aureus (ProtA) and the calmodulin-binding peptide (CBP), and it allows for recovery of 20–30% of the bait protein in yeast. When applied to higher eukaryotes, however, this classical TAP tag suffers from low yields. To improve protein recovery in systems other than yeast, we describe herein the development of a three-tag system comprised of CBP, streptavidin-binding peptide (SBP) and hexa-histidine. We illustrate the application of this approach for the purification of human Bruton's tyrosine kinase (Btk), which results in highly efficient binding and elution of bait protein in both purification steps (>50% recovery). Combined with mass spectrometry for protein identification, this TAP strategy facilitated the first nonbiased analysis of Btk interacting proteins. The high efficiency of the SBP-His6 purification allows for efficient recovery of protein complexes formed with a target protein of interest from a small amount of starting material, enhancing the ability to detect low abundance and transient interactions in eukaryotic cell systems. PMID:21080425

Li, Yifeng; Franklin, Sarah; Zhang, Michael J; Vondriska, Thomas M

2011-01-01

391

Tag loss and short-term mortality associated with passive integrated transponder tagging of juvenile Lost River suckers  

USGS Publications Warehouse

Passive integrated transponder (PIT) tags are commonly used to mark small catostomids, but tag loss and the effect of tagging on mortality have not been assessed for juveniles of the endangered Lost River sucker Deltistes luxatus. I evaluated tag loss and short-term (34-d) mortality associated with the PIT tagging of juvenile Lost River suckers in the laboratory by using a completely randomized design and three treatment groups (PIT tagged, positive control, and control). An empty needle was inserted into each positive control fish, whereas control fish were handled but not tagged. Only one fish expelled its PIT tag. Mortality rate averaged 9.8 ± 3.4% (mean ± SD) for tagged fish; mortality was 0% for control and positive control fish. All tagging mortalities occurred in fish with standard lengths of 71 mm or less, and most of the mortalities occurred within 48 h of tagging. My results indicate that 12.45- × 2.02-mm PIT tags provide a viable method of marking juvenile Lost River suckers that are 72 mm or larger.

Burdick, Summer M.

2011-01-01

392

TAG-MASS: SPECIFIC MOLECULAR IMAGING OF TRANSCRIPTOME AND PROTEOME BY MASS SPECTROMETRY BASED ON PHOTOCLEAVABLE TAG  

E-print Network

1 TAG-MASS: SPECIFIC MOLECULAR IMAGING OF TRANSCRIPTOME AND PROTEOME BY MASS SPECTROMETRY BASED modified with a photocleavable linker coupled with a tag cleaved and detected by Mass Spectrometry. Tag-Mass is difficult however, and presents a real challenge for mass spectrometry. Moreover, as for traditional MALDI

Paris-Sud XI, Université de

393

Fluorescent Boronic Acid Polymer Grafted on Silica Particles for Affinity Separation of Saccharides  

PubMed Central

Boronic acid affinity gels are important for effective separation of biological active cis-diols, and are finding applications both in biotech industry and in biomedical research areas. To increase the efficacy of boronate affinity separation, it is interesting to introduce repeating boronic acid units in flexible polymer chains attached on solid materials. In this work, we synthesize polymer brushes containing boronic acid repeating units on silica gels using surface-initiated atom transfer radical polymerization (ATRP). A fluorescent boronic acid monomer is first prepared from an azide-tagged fluorogenic boronic acid and an alkyne-containing acrylate by Cu(I)-catalyzed 1,3-dipolar cycloaddition reaction (the CuAAC click chemistry). The boronic acid monomer is then grafted to the surface of silica gel modified with an ATRP initiator. The obtained composite material contains boronic acid polymer brushes on surface and shows favorable saccharide binding capability under physiological pH conditions, and displays interesting fluorescence intensity change upon binding fructose and glucose. In addition to saccharide binding, the flexible polymer brushes on silica also enable fast separation of a model glycoprotein based on selective boronate affinity interaction. The synthetic approach and the composite functional material developed in this work should open new opportunities for high efficiency detection, separation, and analysis of not only simple saccharides, but also glycopeptides and large glycoproteins. PMID:24444898

2014-01-01

394

His-tag truncated butyrylcholinesterase as a useful construct for in vitro characterization of wild-type and variant butyrylcholinesterases  

PubMed Central

Human butyrylcholinesterase (BChE) can scavenge and thereby provide protection against various toxic esters, including organophosphate-based chemical warfare agents and the recreational drug cocaine. It is currently being used in molecular evolution studies to generate novel enzymes with improved ability to hydrolyze toxic ester compounds. Currently, the most commonly used purification strategies for recombinant BChE enzymes involve using affinity resins based on small molecule interactions with the enzyme’s substrate binding site. However, as BChE variants are discovered and developed, a generic purification protocol that is insensitive to amino acid substitutions is necessary. In the current manuscript, an expression vector encoding a C-terminal truncation and His6-tag was designed for BChE and used to express recombinant “wild-type” enzyme and two variants (i.e., G117H BChE and G117H/E197Q BChE). All three His6-tagged enzymes were successfully purified via metal-affinity columns using similar procedures with good recovery. Steady-state kinetic parameters were determined for each enzyme, and values were compared to those obtained with the corresponding non-truncated non-His6-tagged enzymes. Rates of inhibition by echothiophate, a model compound for organophosphate-based pesticides, and rates of oxime-mediated reactivation after inhibition with a nerve agent model compound were also determined for selected enzymes. Rates of spontaneous reactivation from ETP inhibition were determined for G117H variants. In all instances examined, truncation of the C-terminus of BChE and introduction of a His6-tag had no significant effects on the observed kinetic parameters, making this a highly useful construct for in vitro characterization of wild-type and variant BChEs. PMID:21802514

Ralph, Erik C.; Xiang, Longkuan; Cashman, John R.; Zhang, Jun

2011-01-01

395

Gas-phase nitronium ion affinities.  

PubMed Central

Evaluation of nitronium ion-transfer equilibria, L1NO2+ + L2 = L2NO2+ + L1 (where L1 and L2 are ligands 1 and 2, respectively) by Fourier-transform ion cyclotron resonance mass spectrometry and application of the kinetic method, based on the metastable fragmentation of L1(NO2+)L2 nitronium ion-bound dimers led to a scale of relative gas-phase nitronium ion affinities. This scale, calibrated to a recent literature value for the NO2+ affinity of water, led for 18 ligands, including methanol, ammonia, representative ketones, nitriles, and nitroalkanes, to absolute NO2+ affinities, that fit a reasonably linear general correlation when plotted vs. the corresponding proton affinities (PAs). The slope of the plot depends to a certain extent on the specific nature of the ligands and, hence, the correlations between the NO2+ affinities, and the PAs of a given class of compounds display a better linearity than the general correlation and may afford a useful tool for predicting the NO2+ affinity of a molecule based on its PA. The NO2+ binding energies are considerably lower than the corresponding PAs and well below the binding energies of related polyatomic cations, such as NO+, a trend consistent with the available theoretical results on the structure and the stability of simple NO2+ complexes. The present study reports an example of extension of the kinetic method to dimers, such as L1(NO2+)L2, bound by polyatomic ions, which may considerably widen its scope. Finally, measurement of the NO2+ affinity of ammonia allowed evaluation of the otherwise inaccessible PA of the amino group of nitramide and, hence, direct experimental verification of previous theoretical estimates. PMID:11607578

Cacace, F; de Petris, G; Pepi, F; Angelelli, F

1995-01-01

396

RFID Label Tag Design for Metallic Surface Environments  

PubMed Central

This paper describes a metal mount RFID tag that works reliably on metallic surfaces. The method proposes the use of commercial label type RFID tags with 2.5 mm thick Styrofoam103.7 with a relative permittivity of 1.03 attached on the back of the tag. In order to verify the performance of the proposed method, we performed experiments on an electric transformer supply chain system. The experimental results showed that the proposed tags can communicate with readers from a distance of 2 m. The recognition rates are comparable to those of commercial metallic mountable tags. PMID:22346612

Park, Chong Ryol; Eom, Ki Hwan

2011-01-01

397

Uncertainty of exploitation estimates made from tag returns  

USGS Publications Warehouse

Over 6,000 crappies Pomoxis spp. were tagged in five water bodies to estimate exploitation rates by anglers. Exploitation rates were computed as the percentage of tags returned after adjustment for three sources of uncertainty: postrelease mortality due to the tagging process, tag loss, and the reporting rate of tagged fish. Confidence intervals around exploitation rates were estimated by resampling from the probability distributions of tagging mortality, tag loss, and reporting rate. Estimates of exploitation rates ranged from 17% to 54% among the five study systems. Uncertainty around estimates of tagging mortality, tag loss, and reporting resulted in 90% confidence intervals around the median exploitation rate as narrow as 15 percentage points and as broad as 46 percentage points. The greatest source of estimation error was uncertainty about tag reporting. Because the large investments required by tagging and reward operations produce imprecise estimates of the exploitation rate, it may be worth considering other approaches to estimating it or simply circumventing the exploitation question altogether.

Miranda, L.E.; Brock, R.E.; Dorr, B.S.

2002-01-01

398

Lightweight Distance bound Protocol for Low Cost RFID Tags  

E-print Network

Almost all existing RFID authentication schemes (tag/reader) are vulnerable to relay attacks, because of their inability to estimate the distance to the tag. These attacks are very serious since it can be mounted without the notice of neither the reader nor the tag and cannot be prevented by cryptographic protocols that operate at the application layer. Distance bounding protocols represent a promising way to thwart relay attacks, by measuring the round trip time of short authenticated messages. All the existing distance bounding protocols use random number generator and hash functions at the tag side which make them inapplicable at low cost RFID tags. This paper proposes a lightweight distance bound protocol for low cost RFID tags. The proposed protocol based on modified version of Gossamer mutual authentication protocol. The implementation of the proposed protocol meets the limited abilities of low-cost RFID tags.

Ahmed, Eslam Gamal; Hashem, Mohamed

2010-01-01

399

Selected Isotopes for Optimized Fuel Assembly Tags  

SciTech Connect

In support of our ongoing signatures project we present information on 3 isotopes selected for possible application in optimized tags that could be applied to fuel assemblies to provide an objective measure of burnup. 1. Important factors for an optimized tag are compatibility with the reactor environment (corrosion resistance), low radioactive activation, at least 2 stable isotopes, moderate neutron absorption cross-section, which gives significant changes in isotope ratios over typical fuel assembly irradiation levels, and ease of measurement in the SIMS machine 2. From the candidate isotopes presented in the 3rd FY 08 Quarterly Report, the most promising appear to be Titanium, Hafnium, and Platinum. The other candidate isotopes (Iron, Tungsten, exhibited inadequate corrosion resistance and/or had neutron capture cross-sections either too high or too low for the burnup range of interest.

Gerlach, David C.; Mitchell, Mark R.; Reid, Bruce D.; Gesh, Christopher J.; Hurley, David E.

2008-10-01

400

Analysis of STIS time-tag data  

NASA Technical Reports Server (NTRS)

Very high time resolution data can be obtained from the Space Telescope Imaging Spectrograph (STIS) Multi-Anode Microchannel Array (MAMA) detectors using the time-tag observing mode. In this mode, the photon events are not accumulated onboard the spacecraft. Instead, each event is recorded internally and transmitted to the ground as an X and Y location with an event time. Event times are recorded in units of 125 microseconds. Analysis of STIS Crab Pulsar data demonstrates that a time resolution of approaching 125 microseconds can be achieved. Furthermore, the time-tag observing mode has been demonstrated to be a very powerful diagnostic tool and can be used to increase the resolution of both imaging and spectral data.

Lindler, Don J.; Gull, Theodore R.; Kraemer, Steven B.; Hulbert, Stephen J.

1997-01-01

401

Chemoenzymatic Fc Glycosylation via Engineered Aldehyde Tags  

PubMed Central

Glycoproteins with chemically defined glycosylation sites and structures are important biopharmaceutical targets and critical tools for glycobiology. One approach toward constructing such molecules involves chemical glycosylation of aldehyde-tagged proteins. Here, we report the installation of a genetically encoded aldehyde tag at the internal glycosylation site of the crystallizable fragment (Fc) of IgG1. We replaced the natural Fc N-glycosylation sequon with a five amino-acid sequence that was efficiently converted by recombinant formylglycine generating enzyme in vitro, thereby introducing aldehyde groups for subsequent chemical elaboration. Oxime-linked glycoconjugates were synthesized by conjugating aminooxy N-acetylglucosamine to the modified Fc followed by enzymatic transfer of complex N-glycans from corresponding glycan oxazolines by an EndoS-derived glycosynthase. In this manner we generated specific Fc glycoforms without relying on natural protein glycosylation machineries. PMID:24702330

2014-01-01

402

Vulnerability Analysis of PAP for RFID Tags  

E-print Network

In this paper, we analyze the security of an RFID authentication protocol proposed by Liu and Bailey [1], called Privacy and Authentication Protocol (PAP), and show its vulnerabilities and faulty assumptions. PAP is a privacy and authentication protocol designed for passive tags. The authors claim that the protocol, being resistant to commonly assumed attacks, requires little computation and provides privacy protection and authentication. Nevertheless, we propose two traceability attacks and an impersonation attack, in which the revealing of secret information (i.e., secret key and static identifier) shared between the tag and the reader is unnecessary. Moreover, we review all basic assumptions on which the design of the protocol resides, and show how many of them are incorrect and are contrary to the common assumptions in RFID systems.

Naser, Mu'awya; Rafie, Mohammd; van der Lubbe, Jan

2010-01-01

403

The connection between metal ion affinity and ligand affinity in integrin I domains  

E-print Network

The connection between metal ion affinity and ligand affinity in integrin I domains Thomas Vorup Abstract Integrins are cell-surface heterodimeric proteins that mediate cell­cell, cell­matrix, and cell­pathogen interactions. Half of the known integrin subunits contain inserted domains (I domains) that coordinate ligand

Springer, Timothy A.

404

Applying Collaborative Tagging to E-Learning  

Microsoft Academic Search

This paper outlines our experiences with applying collaborative tagging in e-learning systems to supplement more traditional metadata gathering approaches. Over the last 10 years, the learning object paradigm has emerged in e-learning and has caused standards bodies to focus on creating metadata repositories based upon strict domain-free taxonomies. We argue that the social collection phenomena and flexible metadata standards are

Scott Bateman; Christopher Brooks; Gordon McCalla; Peter Brusilovsky

2007-01-01

405

A fractal circular polarized RFID tag antenna  

NASA Astrophysics Data System (ADS)

In this paper, we present a novel fractal antenna for radiofrequency identification (RFID) tags. The proposed antenna has a resonant frequency equal to 2.45GHz and circular polarization. The fractal technique was very useful to obtain a miniaturization of antenna size by more than 30%. The gain and directivity of the antenna are acceptable for the desired RFID application. All the results are obtained using CST Microwave simulation tool.

Chaouki, Guesmi; Ferchichi, Abdelhak; Gharsallah, Ali

2013-09-01

406

Morphological Tagging of the Qur'an  

Microsoft Academic Search

We present a computational system for morphological tagging of the Qur'an, for research and teaching purposes. The system facilitates a variety of queries on the Qur'anic text that make reference not only to the words but also to their linguistic attributes. The core of the system is a set of finite-state based rules which describe the morpho-phonological and morpho- syntactic

Rafi Talmon; Shuly Wintner

407

Scanning Cargo Containers with Tagged Neutrons  

NASA Astrophysics Data System (ADS)

A new Tagged Neutron Inspection System (TNIS) able to detect illicit materials such as explosives and narcotics in cargo containers has been developed within the EURopean Illicit TRAfficing Countermeasures Kit (EURITRACK) project. After the R&D phase, the inspection portal has been installed and commissioned at the Rijeka seaport in Croatia, where it has been operated in connection with the existing X-ray scanner for a first two-month demonstration campaign. Results obtained are presented and discussed in this paper.

Viesti, G.; Botosso, C.; Fabris, D.; Lunardon, M.; Moretto, S.; Nebbia, G.; Pesente, S.; Zenoni, A.; Donzella, A.; Perot, B.; Carasco, C.; Bernard, S.; Mariani, A.; Szabo, J.-L.; Sannie, G.; Valkovic, V.; Sudac, D.; Nad, K.; Peerani, P.; Sequeira, V.; Salvato, M.; Moszynski, M.; Gierlik, M.; Klamra, W.; Le Tourneur, P.; Lhuissier, M.; Colonna, A.; Tintori, C.

2007-10-01

408

An approach to sequence DNA without tagging  

NASA Astrophysics Data System (ADS)

Microarray technology is playing an increasingly important role in biology and medicine and its application to genomics for gene expression analysis has already reached the market with a variety of commercially available instruments. In these combinatorial analysis methods, known probe single-strand DNA (ssDNA) 'primers' are attached in clusters of typically 100 µm × 100 µm pixels. Each pixel of the array has a slightly different sequence. On exposure to 'unknown' target ssDNA, the pixels with the right complementary probe ssDNA sequence convert to double-stranded DNA (dsDNA) by a hybridization reaction. To transduct the conversion of the pixel to dsDNA, the target ssDNA is labelled with a photoluminescent tag during the polymerase chain reaction (PCR) amplification process. Due to the statistical distribution of the tags in the target ssDNA, it becomes significantly difficult to implement these methods as a diagnostic tool in a pathology laboratory. A method to sequence DNA without tagging the molecule is developed. The fabrication process is compatible with current microelectronics and (emerging) soft-material fabrication technologies, allowing the method to be integrable with micro-electromechanical systems (MEMS) and lab-on-a-chip devices. An estimated sensitivity of 10-12 g on a 1 cm2 device area is obtained.

Niu, Sanjun; Saraf, Ravi F.

2002-10-01

409

TagRecon: High-Throughput Mutation Identification through Sequence Tagging  

PubMed Central

Shotgun proteomics produces collections of tandem mass spectra that contain all the data needed to identify mutated peptides from clinical samples. Identifying these sequence variations, however, has not been feasible with conventional database search strategies, which require exact matches between observed and expected sequences. Searching for mutations as mass shifts on specified residues through database search can incur significant performance penalties and generate substantial false positive rates. Here we describe TagRecon, an algorithm that leverages inferred sequence tags to identify unanticipated mutations in clinical proteomic data sets. TagRecon identifies unmodified peptides as sensitively as the related MyriMatch database search engine. In both LTQ and Orbitrap data sets, TagRecon outperformed state of the art software in recognizing sequence mismatches from data sets with known variants. We developed guidelines for filtering putative mutations from clinical samples, and we applied them in an analysis of cancer cell lines and an examination of colon tissue. Mutations were found in up to 6% of identified peptides, and only a small fraction corresponded to dbSNP entries. The RKO cell line, which is DNA mismatch repair deficient, yielded more mutant peptides than the mismatch repair proficient SW480 line. Analysis of colon cancer tumor and adjacent tissue revealed hydroxyproline modifications associated with extracellular matrix degradation. These results demonstrate the value of using sequence tagging algorithms to fully interrogate clinical proteomic data sets. PMID:20131910

Dasari, Surendra; Chambers, Matthew C.; Slebos, Robbert J.; Zimmerman, Lisa J.; Ham, Amy-Joan L.; Tabb, David L.

2010-01-01

410

The dynamics of metric-affine gravity  

SciTech Connect

Highlights: > The role and the dynamics of the connection in metric-affine theories is explored. > The most general second order action does not lead to a dynamical connection. > Including higher order invariants excites new degrees of freedom in the connection. > f(R) actions are also discussed and shown to be a non- representative class. - Abstract: Metric-affine theories of gravity provide an interesting alternative to general relativity: in such an approach, the metric and the affine (not necessarily symmetric) connection are independent quantities. Furthermore, the action should include covariant derivatives of the matter fields, with the covariant derivative naturally defined using the independent connection. As a result, in metric-affine theories a direct coupling involving matter and connection is also present. The role and the dynamics of the connection in such theories is explored. We employ power counting in order to construct the action and search for the minimal requirements it should satisfy for the connection to be dynamical. We find that for the most general action containing lower order invariants of the curvature and the torsion the independent connection does not carry any dynamics. It actually reduces to the role of an auxiliary field and can be completely eliminated algebraically in favour of the metric and the matter field, introducing extra interactions with respect to general relativity. However, we also show that including higher order terms in the action radically changes this picture and excites new degrees of freedom in the connection, making it (or parts of it) dynamical. Constructing actions that constitute exceptions to this rule requires significant fine tuned and/or extra a priori constraints on the connection. We also consider f(R) actions as a particular example in order to show that they constitute a distinct class of metric-affine theories with special properties, and as such they cannot be used as representative toy theories to study the properties of metric-affine gravity.

Vitagliano, Vincenzo, E-mail: vitaglia@sissa.it [SISSA-International School for Advanced Studies, Via Bonomea 265, 34136 Trieste (Italy); INFN, Sez. di Trieste, Via Valerio 2, 34127 Trieste (Italy); Sotiriou, Thomas P., E-mail: T.Sotiriou@damtp.cam.ac.uk [Department of Applied Mathematics and Theoretical Physics, Centre for Mathematical Sciences, University of Cambridge, Wilberforce Road, Cambridge, CB3 0WA (United Kingdom); Liberati, Stefano, E-mail: liberati@sissa.it [SISSA-International School for Advanced Studies, Via Bonomea 265, 34136 Trieste (Italy); INFN, Sez. di Trieste, Via Valerio 2, 34127 Trieste (Italy)

2011-05-15

411

Elusive electron affinity of ClF  

NASA Astrophysics Data System (ADS)

Highly correlated methods were used to obtain the optimized bond lengths and vibrational frequencies of ClF and ClF-. With convergent quantum mechanical methods, the anion is much more difficult to treat than neutral ClF. Adiabatic electron affinities (EAad), vertical electron affinities, and vertical detachment energies have been evaluated and compared to the controversial experimental values reported in the literature. Our best prediction for the zero-point vibrationally corrected EAad is 2.25±0.1 eV.

Horný, ?uboš; Sattelmeyer, Kurt W.; Schaefer, Henry F.

2003-12-01

412

Negative Electron Affinity Mechanism for Diamond Surfaces  

NASA Technical Reports Server (NTRS)

The energy distribution of the secondary electrons for chemical vacuum deposited diamond films with Negative Electron Affinity (NEA) was investigated. It was found that while for completely hydrogenated diamond surfaces the negative electron affinity peak in the energy spectrum of the secondary electrons is present for any energy of the primary electrons, for partially hydrogenated diamond surfaces there is a critical energy above which the peak is present in the spectrum. This critical energy increases sharply when hydrogen coverage of the diamond surface diminishes. This effect was explained by the change of the NEA from the true type for the completely hydrogenated surface to the effective type for the partially hydrogenated surfaces.

Krainsky, I. L.; Asnin, V. M.

1998-01-01

413

Translating Ribosome Affinity Purification (TRAP) Followed by RNA Sequencing Technology (TRAP-SEQ) for Quantitative Assessment of Plant Translatomes.  

PubMed

Translating Ribosome Affinity Purification (TRAP) is a technology to isolate the population of mRNAs associated with at least one 80S ribosome, referred as the translatome. TRAP is based on the expression of an epitope-tagged version of a ribosomal protein and the affinity purification of ribosomes and associated mRNAs using antibodies conjugated to agarose beads. Quantitative assessment of the translatome is achieved by direct RNA sequencing (RNA-SEQ), which provides accurate quantitation of ribosome-associated mRNAs and reveals alternatively spliced isoforms. Here we present a detailed procedure for TRAP, as well as a guide for preparation of RNA-SEQ libraries (TRAP-SEQ) and a primary data analysis. This methodology enables the study of translational dynamic by assessing rapid changes in translatomes, at organ or cell-type level, during development or in response to endogenous or exogenous stimuli. PMID:25757773

Reynoso, Mauricio A; Juntawong, Piyada; Lancia, Marcos; Blanco, Flavio A; Bailey-Serres, Julia; Zanetti, María Eugenia

2015-01-01

414

Tags, wireless communication systems, tag communication methods, and wireless communications methods  

DOEpatents

Tags, wireless communication systems, tag communication methods, and wireless communications methods are described. In one aspect, a tag includes a plurality of antennas configured to receive a plurality of first wireless communication signals comprising data from a reader, a plurality of rectifying circuits coupled with. respective individual ones of the antennas and configured to provide rectified signals corresponding to the first wireless communication signals, wherein the rectified signals are combined to produce a composite signal, an adaptive reference circuit configured to vary a reference signal responsive to the composite signal, a comparator coupled with the adaptive reference circuit and the rectifying circuits and configured to compare the composite signal with respect to the reference signal and to output the data responsive to the comparison, and processing circuitry configured to receive the data from the comparator and to process the data.

Scott; Jeff W. (Pasco, WA), Pratt; Richard M. (Richland, WA)

2006-09-12

415

Learning to rank image tags with limited training examples.  

PubMed

With an increasing number of images that are available in social media, image annotation has emerged as an important research topic due to its application in image matching and retrieval. Most studies cast image annotation into a multilabel classification problem. The main shortcoming of this approach is that it requires a large number of training images with clean and complete annotations in order to learn a reliable model for tag prediction. We address this limitation by developing a novel approach that combines the strength of tag ranking with the power of matrix recovery. Instead of having to make a binary decision for each tag, our approach ranks tags in the descending order of their relevance to the given image, significantly simplifying the problem. In addition, the proposed method aggregates the prediction models for different tags into a matrix, and casts tag ranking into a matrix recovery problem. It introduces the matrix trace norm to explicitly control the model complexity, so that a reliable prediction model can be learned for tag ranking even when the tag space is large and the number of training images is limited. Experiments on multiple well-known image data sets demonstrate the effectiveness of the proposed framework for tag ranking compared with the state-of-the-art approaches for image annotation and tag ranking. PMID:25622318

Songhe Feng; Zheyun Feng; Rong Jin

2015-04-01

416

Integrated Management and Visualization of Electronic Tag Data with Tagbase  

PubMed Central

Electronic tags have been used widely for more than a decade in studies of diverse marine species. However, despite significant investment in tagging programs and hardware, data management aspects have received insufficient attention, leaving researchers without a comprehensive toolset to manage their data easily. The growing volume of these data holdings, the large diversity of tag types and data formats, and the general lack of data management resources are not only complicating integration and synthesis of electronic tagging data in support of resource management applications but potentially threatening the integrity and longer-term access to these valuable datasets. To address this critical gap, Tagbase has been developed as a well-rounded, yet accessible data management solution for electronic tagging applications. It is based on a unified relational model that accommodates a suite of manufacturer tag data formats in addition to deployment metadata and reprocessed geopositions. Tagbase includes an integrated set of tools for importing tag datasets into the system effortlessly, and provides reporting utilities to interactively view standard outputs in graphical and tabular form. Data from the system can also be easily exported or dynamically coupled to GIS and other analysis packages. Tagbase is scalable and has been ported to a range of database management systems to support the needs of the tagging community, from individual investigators to large scale tagging programs. Tagbase represents a mature initiative with users at several institutions involved in marine electronic tagging research. PMID:21750734

Lam, Chi Hin; Tsontos, Vardis M.

2011-01-01

417

Development of techniques for tagging precursor and essential chemicals  

SciTech Connect

The ability to identify the manufacturers and distributors of chemicals seized in raids of illicit drug labs would be of great value in controlling the diversion of these chemicals. We developed a tagging scheme based on the addition of sub-ppM concentrations of various combinations of rare-earth elements to the target chemicals and evaluated a number of techniques for detecting the tags. We developed soluble tags for tagging liquids and selected Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) as the preferred detection technique. We developed insoluble tags for tagging solids and developed methods to analyze them and mix them into solid precursors. We have successfully demonstrated the tagging of several solvents and two of the precursor chemicals used in one of the most popular clandestine methamphetamine syntheses (ephedrine reacting with hydriodic acid/red phosphorus). The tagging scheme is capable of yielding tens of thousands of signatures (using holmium as an internal standard and up to 9 rare-earths at up to 3 concentrations yields 3{sup 9} {minus} 1 = 19,682 signatures) and is applicable to most of the chemicals on the precursor and essential chemicals list. In the concentrations employed, the tags are safe enough to be added to pharmaceuticals and cheap enough to tag tanker loads of chemicals.

Swansiger, W.A.; Shepodd, T.J. [Sandia National Labs., Livermore, CA (United States); Phillips, M.L.F. [Sandia National Labs., Albuquerque, NM (United States)

1994-01-01

418

Some Fundamental Limits on SAW RFID Tag Information Capacity and Collision Resolution  

NASA Technical Reports Server (NTRS)

In this paper, we apply results from multi-user information theory to study the limits of information capacity and collision resolution for SAW RFID tags. In particular, we derive bounds on the achievable data rate per tag as a function of fundamental parameters such as tag time-bandwidth product, tag signal-to-noise ratio (SNR), and number of tags in the environment. We also discuss the implications of these bounds for tag waveform design and tag interrogation efficiency

Barton, Richard J.

2013-01-01

419

Affine Lie Algebras and Tame Quivers  

Microsoft Academic Search

C.M. Ringel defined Hall algebra associated with the category of representations of a quiver of Dynkin type and gave an explicit description of the structure constants of the corresponding Lie algebra. We utilize functorial properties of the Hall algebra to give a simple proof of Ringel's result, and to generalize it to the case of a quiver of affine type.

Igor Frenkel; Anton Malkin; Maxim Vybornov

2000-01-01

420

Application of affinity adsorption in thienamycin fermentation  

Microsoft Academic Search

Many antibiotic fermentations are sensitive to high concentrations of their own product possibly due to product regulation and toxicity mechanisms. In this paper we discuss the feasibility of using affinity adsorption with biospecific ligands for in situ product removal to alleviate this problem. The concept of using whole cells containing the biospecific ligands is demonstrated in the case of thienamycin

Henry Y. Wang; Srinivas Palanki; Gregory S. Hyatt

1989-01-01

421

Affinity Chromatography Media CellufineTM Sulfate  

E-print Network

diagnostics have created an increasing demand for large volumes of highly purified and concentrated virus Table 1. Table 1 Viruses Rabies* Influenza* Japanese Enchephalitis* Feline Leukemia Feline Herpes Feline and Depyrogenation of Virus, Viral/Microbial Antigens, Heparin Binding Proteins D A T A S H E E T FEATURES · Affinity

Lebendiker, Mario

422

RECOGNIZING AND PARAMETRIZING CURVES WITHOUT AFFINE SINGULARITIES  

E-print Network

Kong RGC-CERG grant. 1 #12;2 CHI-MING LAM, VLADIMIR SHPILRAIN, AND JIE-TAI YU The purposeRECOGNIZING AND PARAMETRIZING CURVES WITHOUT AFFINE SINGULARITIES CHI-MING LAM, VLADIMIR SHPILRAIN, AND JIE-TAI YU Abstract. Some time ago, Shpilrain and Yu reported an algorithm for deciding whether

Shpilrain, Vladimir

423

Localization of Affine W-Algebras  

NASA Astrophysics Data System (ADS)

We introduce the notion of an asymptotic algebra of chiral differential operators. We then construct, via a chiral Hamiltonian reduction, one such algebra over a resolution of the intersection of the Slodowy slice with the nilpotent cone. We compute the space of global sections of this algebra, thereby proving a localization theorem for affine W-algebras at the critical level.

Arakawa, T.; Kuwabara, T.; Malikov, F.

2015-04-01

424

Fan Affinity Laws from a Collision Model  

ERIC Educational Resources Information Center

The performance of a fan is usually estimated using hydrodynamical considerations. The calculations are long and involved and the results are expressed in terms of three affinity laws. In this paper we use kinetic theory to attack this problem. A hard sphere collision model is used, and subsequently a correction to account for the flow behaviour…

Bhattacharjee, Shayak

2012-01-01

425

Affinity-Based Purification of Dehydrogenase Subproteomes  

PubMed Central

Summary The high cost of drug discovery and development requires more efficient approaches to the identification and inhibition of tractable protein targets. One strategy is to pursue families of proteins that already possess affinity for a drug lead scaffold, where that scaffold plays the dual role of serving: (a) when tethered to a resin, as a ligand to purify a subproteome of interest, and (b) as a lead molecule that has the potential for optimization for a given member of the subproteome. Here, we describe the former application, the purification of a subproteome using a scaffold tailored to the dehydrogenase family of enzymes. Combined with modern LC-MS/MS and subsequent searching of proteome databases, such affinity chromatography strategies can be used to purify and identify any proteins with affinity for the scaffold molecule. The method is exemplified using the CRAA (Catechol Rhodanine Acetic Acid) privileged scaffold, which is tailored to dehydrogenases. CRAA affinity column chromatography, combined with LC-MS/MS, is described as a method for profiling dehydrogenase subproteomes. PMID:22065224

Ge, Xia; Sem, Daniel S.

2014-01-01

426

Cluster algebras and quantum affine algebras  

Microsoft Academic Search

Let ${\\\\mathcal C}$ be the category of finite-dimensional representations of a quantum affine algebra\\u000a$U_q(\\\\widehat{\\\\mathfrak g})$ of simply laced type. We introduce certain monoidal subcategories ${\\\\mathcal C}_\\\\ell (\\\\ell\\\\in{\\\\mathbb N})$ of ${\\\\mathcal C}$ , and we study their Grothendieck rings using cluster algebras.

David Hernandez; Bernard Leclerc

2010-01-01

427

AFFINITY ENRICHMENT OF BOVINE LACTOFERRIN IN WHEY  

Microsoft Academic Search

Bovine lactoferrin was enriched in various whey samples by affinity chromatography using immobilized gangliosides. Bovine gangliosides were isolated from fresh buttermilk using a combination of ultrafiltration and organic extraction. Isolated gangliosides were covalently immobilized onto controlled-pore glass beads. The immobilized matrix contained 66 micrograms of gangliosides per gram of beads. After loading the matrix with reconstituted whey protein isolate (WPI)

M. K. Walsh; S. H. Nam

2001-01-01

428

Vygotsky's and Buber's Pedagogical Perspectives: Some Affinities  

ERIC Educational Resources Information Center

The purpose of this paper is to examine the dialogical and creative character of pedagogic work by analyzing the affinities between Martin Buber's "I-Thou relation" and Lev Semenovich Vygotsky's "Zone of Proximal Development". Backed up by empirical studies on the teacher-student relation, we understand that education can only result in students'…

Bartholo, Roberto; Tunes, Elizabeth; Tacca, Maria Carmen Villela Rosa

2010-01-01

429

Method for nonlinear optimization for gas tagging and other systems  

DOEpatents

A method and system for providing nuclear fuel rods with a configuration of isotopic gas tags. The method includes selecting a true location of a first gas tag node, selecting initial locations for the remaining n-1 nodes using target gas tag compositions, generating a set of random gene pools with L nodes, applying a Hopfield network for computing on energy, or cost, for each of the L gene pools and using selected constraints to establish minimum energy states to identify optimal gas tag nodes with each energy compared to a convergence threshold and then upon identifying the gas tag node continuing this procedure until establishing the next gas tag node until all remaining n nodes have been established.

Chen, Ting (Chicago, IL); Gross, Kenny C. (Bolingbrook, IL); Wegerich, Stephan (Glendale Heights, IL)

1998-01-01

430

An overview of radio frequency identification (RFID) tags technology  

NASA Astrophysics Data System (ADS)

RFID (Radio Frequency Identification) is the technology of wireless identification of tagged products. It is one of the fastest developing technologies in electronic market and it is predicted to replace soon the barcodes which are in common usage in today's economy. There are several advantages of RFID tags over barcode. The main are reading without must of scanning the product and the possibility to keep much more information on chip of the tag. In the article there are introduced the possible applications of RFID technology. There are also presented the classification of the RFID tags and the difference between working frequency. It is introduced every steps of manufacturing RFID tags with focus on the technology aspects (technologies of producing antenna, attaching the chip and creation of electrical connection between antenna and chip). Tele and Radio Research Institute is now starting to realize the project of manufacturing the RFID tags antenna. There is presented our guideline of the project.

Falinski, Wojciech

2006-10-01

431

Method for nonlinear optimization for gas tagging and other systems  

DOEpatents

A method and system are disclosed for providing nuclear fuel rods with a configuration of isotopic gas tags. The method includes selecting a true location of a first gas tag node, selecting initial locations for the remaining n-1 nodes using target gas tag compositions, generating a set of random gene pools with L nodes, applying a Hopfield network for computing on energy, or cost, for each of the L gene pools and using selected constraints to establish minimum energy states to identify optimal gas tag nodes with each energy compared to a convergence threshold and then upon identifying the gas tag node continuing this procedure until establishing the next gas tag node until all remaining n nodes have been established. 6 figs.

Chen, T.; Gross, K.C.; Wegerich, S.

1998-01-06

432

Fluorescent SNAP-tag galectin fusion proteins as novel tools in glycobiology.  

PubMed

Galectins,?-galactoside binding proteins, function in several physiological and pathological processes. The further evaluation of these processes as well as possible applications of galectins in diagnosis and therapy has raised high scientific interest. Therefore, easy and reliable test systems are necessary. Here we present the simple and cost-efficient production of recombinant human galectins as fusion proteins with SNAP-tag and fluorescent proteins. These constructs show binding specificities and oligomerisation properties generally comparable to recombinant galectins. Their direct fluorescence signal was utilised by ELISA-type assay and flow cytometry analysis with human and ovine mesenchymal stem cells (MSC). Flow cytometry demonstrated glycan mediated binding of His6-SNAP-YFP-Gal- 3 to both MSC types, which was specifically inhibited by lactose. Moreover, directed immobilisation by SNAP-tag technology onto benzylguanine- activated sepharose was utilised to prepare galectin affinity columns for glycoprotein analysis and purification. The SNAPtag directed coupling yielded up to three-fold higher binding capacities for the glycoprotein standard asialofetuin compared to nondirected coupled galectin suggesting improved functionality following directed coupling. PMID:23431989

Kupper, Christiane E; Böcker, Sophia; Liu, Hulong; Adamzyk, Carina; van de Kamp, Julia; Recker, Tobias; Lethaus, Bernd; Jahnen-Dechent, Willi; Neuss, Sabine; Müller-Newen, Gerhard; Elling, Lothar

2013-01-01

433

Bicyclic peptides conjugated to an albumin-binding tag diffuse efficiently into solid tumors.  

PubMed

Monoclonal antibodies have long in vivo half-lives and reach high concentrations in tumors but cannot access all regions in the tissue, whereas smaller ligands such as peptides distribute better but are limited by low concentrations due to fast renal clearance. A potential solution to this problem might be offered by peptide-based ligands that are conjugated to an albumin-binding tag, and thus have a long plasma half-life. Herein, we tested if a small ligand based on a bicyclic peptide (1.9 kDa) conjugated to an albumin-binding peptide (2.3 kDa) can diffuse into tissues. Although the peptide conjugate (4.6 kDa) was most of the time bound to the large protein serum albumin (66.5 kDa), it diffused deeply into tissues and reached high nanomolar concentrations in wide areas of solid tumors. Most of the peptide conjugate isolated from tumor tissue was found to be fully intact 24 hours after administration. Because of its noncovalent interaction with albumin, the bicyclic peptide might dissociate to diffuse to tumor regions that are not accessible to larger ligands. Bicyclic peptides having high binding affinity for targets of interest and being proteolytically stable can be evolved by phage display; in conjunction with albumin-binding tags, they offer a promising format to access targets in solid tumors. PMID:25381263

Pollaro, Lisa; Raghunathan, Sandeep; Morales-Sanfrutos, Julia; Angelini, Alessandro; Kontos, Stephan; Heinis, Christian

2015-01-01

434

A generic method for expression and use of "tagged" soluble versions of cell surface receptors.  

PubMed

A general method for expression, purification, immobilization, detection and radiolabeling of extracellular domains (ECD) of type I membrane proteins. The type I interleukin-1 receptor (IL-1RtI), the alpha-subunit of interleukin-2 receptor (IL-2R alpha) and E-selectin are used as illustrative examples of cell surface receptors. DNA encoding the ECD of the proteins are fused at their 3' end to a chimeric DNA which serves to generically "tag" the recombinant ECD. The resulting fusion protein contains a substrate sequence for protein kinase-A (PKA) adjacent to the signal sequence from human placental alkaline phosphatase (HPAP), The HPAP signal sequence directs the formation of the phosphatidylinositol-glycan (PI-G) anchorage of the protein at the cell surface. When these chimeric genes are expressed in CHO cells, the ECDs are detected on the cell surface and can be released by treatment with phosphatidylinositol-specific phospholipase-C (PI-PLC). Based on protein processing known to occur for native HPAP, twenty amino acids from the HPAP signal sequence remain at the C-terminus of the ECD. A high affinity monoclonal antibody was generated against this common epitope. This antibody can be used to detect, purify and immobilize the ECDs. In addition, the ECDs can be radiolabeled with 32P by treatment with PKA and maintain the ability to bind their natural ligands. This "tagging" method has been successfully applied to many other type I proteins which serve as cell surface receptors. PMID:9636295

Whitehorn, E A; Tate, E; Yanofsky, S D; Kochersperger, L; Davis, A; Mortensen, R B; Yonkovich, S; Bell, K; Dower, W J; Barrett, R W

1995-11-01

435

A novel method for high-level production of TEV protease by superfolder GFP tag.  

PubMed

Because of its stringent sequence specificity, tobacco etch virus (TEV) protease is widely used to remove fusion tags from recombinant proteins. Due to the poor solubility of TEV protease, many strategies have been employed to increase the expression level of this enzyme. In our work, we introduced a novel method to produce TEV protease by using visible superfolder green fluorescent protein (sfGFP) as the fusion tag. The soluble production and catalytic activity of six variants of sfGFP-TEV was examined, and then the best variant was selected for large-scale production. After purified by Ni-NTA affinity chromatography and Q anion exchange chromatography, the best variant of sfGFP-TEV fusion protease was obtained with purity of over 98% and yield of over 320 mg per liter culture. The sfGFP-TEV had a similar catalytic activity to that of the original TEV protease. Our research showed a novel method of large-scale production of visible and functional TEV protease for structural genomics research and other applications. PMID:20182554

Wu, Xudong; Wu, Di; Lu, Zhisheng; Chen, Wentao; Hu, Xiaojian; Ding, Yu

2009-01-01

436

Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT)  

PubMed Central

The proper dissection of the molecular mechanisms governing the specification and differentiation of specific cell types requires isolation of pure cell populations from heterogeneous tissues and whole organisms. Here, we describe a method for purification of nuclei from defined cell or tissue types in vertebrate embryos using INTACT (isolation of nuclei tagged in specific cell types). This method, previously developed in plants, flies and worms, utilizes in vivo tagging of the nuclear envelope with biotin and the subsequent affinity purification of the labeled nuclei. In this study we successfully purified nuclei of cardiac and skeletal muscle from Xenopus using this strategy. We went on to demonstrate the utility of this approach by coupling the INTACT approach with liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomic methodologies to profile proteins expressed in the nuclei of developing hearts. From these studies we have identified the Xenopus orthologs of 12 human proteins encoded by genes, which when mutated in human lead to congenital heart disease. Thus, by combining these technologies we are able to identify tissue-specific proteins that are expressed and required for normal vertebrate organ development. PMID:24496632

Amin, Nirav M.; Greco, Todd M.; Kuchenbrod, Lauren M.; Rigney, Maggie M.; Chung, Mei-I; Wallingford, John B.; Cristea, Ileana M.; Conlon, Frank L.

2014-01-01

437

Boost search relevance for tag-based social image retrieval  

Microsoft Academic Search

Social media sharing Web sites like Flickr allow users to annotate images with free tags, which greatly facilitate social image search and browsing. However, currently tag-based image search on Flickr does not provide the option of relevance-based ranking, i.e., the search results cannot be ranked according to their relevance levels with respect to the query tag, and this has limited

Dong Liu; Xian-Sheng Hua; Meng Wang; HongJiang Zhang

2009-01-01

438

Minimalist Cryptography for Low-Cost RFID Tags  

Microsoft Academic Search

A radio-frequency identification (RFID) tag is a small, inexpensive microchip that emits an identifier in response to a query from a nearby reader. The price of these tags promises to drop to the range of $0.05 per unit in the next several years, oering a viable and powerful replacement for barcodes. The challenge in providing security for low-cost RFID tags

Ari Juels

2004-01-01

439

Social media filtering based on collaborative tagging in semantic space  

Microsoft Academic Search

We propose a semantic collaborative filtering method to enhance recommendation quality derived from user-generated tags. Social\\u000a tagging is employed as an approach in order to grasp and filter users’ preferences for items. In addition, we explore several\\u000a advantages of semantic tagging for ambiguity, synonymy, and semantic interoperability, which are notable challenges in information\\u000a filtering. The proposed approach first determines semantically

Heung-Nam Kim; Andrew Roczniak; Pierre Lévy; Abdulmotaleb El Saddik

440

Public-Key Cryptography for RFID-Tags  

Microsoft Academic Search

RFID-tags are a new generation of bar-codes with added functionality. They are becom- ing very popular tools for identication of products in various applications like e.g. supply-chain management. An emerging application is the use of RFID-tags for anti-counterfeiting by embedding them into a product. However, there is a risk related to naively using those tags for several applica- tions. In

Lejla Batina; Jorge Guajardo; Tim Kerins; Nele Mentens; Pim Tuyls; Ingrid Verbauwhede

2007-01-01

441

Surface Plasmon Resonance Analysis of Histidine-Tagged F1-ATPase Surface Adsorption  

NASA Astrophysics Data System (ADS)

Studies of the rotational activity of the enzymatic core (?3?3?) of the F1-ATPase motor protein have relied on binding the enzyme to NTA-coated glass surfaces via polyhistidine tags engineered into the C-termini of each of the three ? or ? subunits. Those studies revealed the rotational motion of the central ? subunit by monitoring the motion of attached micron-long actin filaments or spherical nanoparticles. However, only a small percentage of the attached filaments or particles were observed to rotate, likely due, at least in part, to non-uniform surface attachment of the motor proteins. In this study, we have applied surface plasmon resonance to monitor the kinetics and affinity of binding of the His-tagged motor protein to NTA-coated gold sensor surfaces. The binding data, when fit to a heterogeneous binding model, exhibit two sets of adsorption-desorption rate constants with two dissociation constants of 4.0 × 10-9 M and 8.6 × 10-11 M for 6His-?3?3? binding to the nickel ion-activated NTA surface. The data are consistent with mixed attachment of the protein via two (bimodal) and three (trimodal) NTA/Ni2+-His-tag interactions, respectively, with the less stable bimodal interaction dominating. The results provide a partial explanation for the low number of surface-attached F1 motors previously observed in rotation studies and suggest alternative approaches to uniform F1 motor surface attachment for future fabrication of motor-based nanobiodevices and materials.

Tucker, Jenifer K.; Richter, Mark L.; Berrie, Cindy L.

2015-03-01

442

Using an ?-bungarotoxin binding site tag to study GABA A receptor membrane localization and trafficking.  

PubMed

It is increasingly evident that neurotransmitter receptors, including ionotropic GABA A receptors (GABAAR), exhibit highly dynamic trafficking and cell surface mobility(1-7). To study receptor cell surface localization and endocytosis, the technique described here combines the use of fluorescent ?-bungarotoxin with cells expressing constructs containing an ?-bungarotoxin (Bgt) binding site (BBS). The BBS (WRYYESSLEPYPD) is based on the ? subunit of the muscle nicotinic acetylcholine receptor, which binds Bgt with high affinity(8,9). Incorporation of the BBS site allows surface localization and measurements of receptor insertion or removal with application of exogenous fluorescent Bgt, as previously described in the tracking of GABAA and metabotropic GABAB receptors(2,10). In addition to the BBS site, we inserted a pH-sensitive GFP (pHGFP(11)) between amino acids 4 and 5 of the mature GABAAR subunit by standard molecular biology and PCR cloning strategies (see Figure 1)(12). The BBS is 3' of the pH-sensitive GFP reporter, separated by a 13-amino acid alanine/proline linker. For trafficking studies described in this publication that are based on fixed samples, the pHGFP serves as a reporter of total tagged GABAAR subunit protein levels, allowing normalization of the Bgt labeled receptor population to total receptor population. This minimizes cell to cell Bgt staining signal variability resulting from higher or lower baseline expression of the tagged GABAAR subunits. Furthermore the pHGFP tag enables easy identification of construct expressing cells for live or fixed imaging experiments. PMID:24747556

Brady, Megan L; Moon, Charles E; Jacob, Tija C

2014-01-01

443

Bifunctional redox tagging of carbon nanoparticles  

NASA Astrophysics Data System (ADS)

Despite extensive work on the controlled surface modification of carbon with redox moieties, to date almost all available methodologies involve complex chemistry and are prone to the formation of polymerized multi-layer surface structures. Herein, the facile bifunctional redox tagging of carbon nanoparticles (diameter 27 nm) and its characterization is undertaken using the industrial dye Reactive Blue 2. The modification route is demonstrated to be via exceptionally strong physisorption. The modified carbon is found to exhibit both well-defined oxidative and reductive voltammetric redox features which are quantitatively interpreted. The method provides a generic approach to monolayer modifications of carbon and carbon nanoparticle surfaces.

Poon, Jeffrey; Batchelor-McAuley, Christopher; Tschulik, Kristina; Palgrave, Robert G.; Compton, Richard G.

2015-01-01

444

Integrated development of up- and downstream processes supported by the Cherry-Tag™ for real-time tracking of stability and solubility of proteins.  

PubMed

Product analytics is the bottleneck of most processes in bioprocess engineering, as it is rather time-consuming. Real-time and in-line product tracing without sample pre-treatment is only possible for few products. The Cherry-Tag™ (Delphi Genetics, Belgium) which can be fused to any target protein allows for straightforward product analytics by VIS absorption measurements. When the fused protein becomes unstable or insoluble, the chromophore function of the group is lost, which makes this technology an ideal screening tool for solubility and stability in up- and downstream process development. The Cherry-Tag™ technology will be presented for the tagged enzyme glutathione-S-transferase (GST) from Escherichia coli in a combined up- and downstream process development study. High-throughput cultivations were carried out in a 48-well format in a BioLector system (m2p-Labs, Germany). The best cultivation setup of highest product titer was scaled up to a 2.5L shake flask culture, followed by a selective affinity chromatography product capturing step. In upstream applications the tag was capable of identifying conditions where insoluble and non-native inclusion bodies were formed. In downstream applications the red-colored product was found to be bound effectively to a GST affinity column. Thus, it was identified to be a native and active protein, as the binding mechanism relies on catalytic activity of the enzyme. The Cherry-Tag™ was found to be a reliable and quantitative tool for real-time tracking of stable and soluble proteins in up- and downstream processing applications. Denaturation and aggregation of the product can be detected in-line at any stage of the process. Critical stages can be identified and subsequently changed or replaced. PMID:25747171

Baumann, Pascal; Bluthardt, Nicolai; Renner, Sarah; Burghardt, Hannah; Osberghaus, Anna; Hubbuch, Jürgen

2015-04-20

445

SRV-TAGS: An Automatic TAGging and Search System for Sensor-Rich Outdoor Videos  

E-print Network

sensors, mobile video, geospatial 1. INTRODUCTION Tagging is one of the most popular methods for users we created geospatial video acquisition applica- tions for both Android- and iOS-based mobile phones Arslan Ay, Seon Ho Kim School of Computing, National University of Singapore, Singapore 117417 Integrated

Kim, Seon Ho

446

Models for tagging data that allow for incomplete mixing of newly tagged animals  

E-print Network

Abstract: The Brownie models for tagging data allow one to estimate age- and year-specific total survival Brownie pour les données de marquage permettent d'évaluer le taux de survie totale propre à un âge et à en compte cette situation. [Traduit par la Rédaction] Introduction Brownie et al. (1978, 1985

Newman, Michael C.

447

Stereospecific analysis of TAG from sunflower seed oil  

Microsoft Academic Search

Stereospecific analysis of TAG from a sunflower seed oil of Tunisian origin was performed. The TAG were first fractionated\\u000a according to chain length and degree of unsaturation by RP-HPLC. The four major diacid- and triacid-TAG fractions were palmitoyldilinoleoyl-glycerol,\\u000a dioleoyllinoleoylglycerol, oleoyldilinoleoylglycerol, and palmitoyloleoyl-linoleoyl-glycerol, amounting to 7.2, 16.6, 29.5,\\u000a and 12 mol%, respectively. The TAG of the four fractions were individually submitted

Sadok Boukhchina; Joseph Gresti; Habib Kallel; Jean Bézard

2003-01-01

448

Optical ID tags for automatic vehicle identification and authentication  

NASA Astrophysics Data System (ADS)

We review the potential of optical techniques in security tasks and propose to combine some of them in the design of new optical ID tags for automatic vehicle identification and authentication. More specifically, we propose to combine visible and near infrared imaging, optical decryption, distortion-invariant ID tags, optoelectronic devices, coherent image processor, optical correlation, and multiple authenticators. A variety of images and signatures, including biometric and random sequences, can be combined in an optical ID tag for multifactor identification. Encryption of the information codified in the ID tag allows increasing security and deters from unauthorized usage of optical tags. A novel NIR ID tag is designed and built by using commonly available materials. The ID tag content cannot be visually perceived at naked eye; it cannot be copied, scanned, or captured by any conventional device. The identification process encompasses several steps such as detection, information decoding and verification which are all detailed in this work. Design of rotation and scale invariant ID tags is taken into account to achieve a correct authentication even if the ID tag is captured in different positions.

Javidi, Bahram; Pérez-Cabré, Elisabet; Millán, María S.

2008-03-01

449

Superior triacylglycerol (TAG) accumulation in starchless mutants of Scenedesmus obliquus: (II) evaluation of TAG yield and productivity in controlled photobioreactors  

PubMed Central

Background Many microalgae accumulate carbohydrates simultaneously with triacylglycerol (TAG) upon nitrogen starvation, and these products compete for photosynthetic products and metabolites from the central carbon metabolism. As shown for starchless mutants of the non-oleaginous model alga Chlamydomonas reinhardtii, reduced carbohydrate synthesis can enhance TAG production. However, these mutants still have a lower TAG productivity than wild-type oleaginous microalgae. Recently, several starchless mutants of the oleaginous microalga Scenedesmus obliquus were obtained which showed improved TAG content and productivity. Results The most promising mutant, slm1, is compared in detail to wild-type S. obliquus in controlled photobioreactors. In the slm1 mutant, the maximum TAG content increased to 57?±?0.2% of dry weight versus 45?±?1% in the wild type. In the wild type, TAG and starch were accumulated simultaneously during initial nitrogen starvation, and starch was subsequently degraded and likely converted into TAG. The starchless mutant did not produce starch and the liberated photosynthetic capacity was directed towards TAG synthesis. This increased the maximum yield of TAG on light by 51%, from 0.144?±?0.004 in the wild type to 0.217?±?0.011 g TAG/mol photon in the slm1 mutant. No differences in photosynthetic efficiency between the slm1 mutant and the wild type were observed, indicating that the mutation specifically altered carbon partitioning while leaving the photosynthetic capacity unaffected. Conclusions The yield of TAG on light can be improved by 51% by using the slm1 starchless mutant of S. obliquus, and a similar improvement seems realistic for the areal productivity in outdoor cultivation. The photosynthetic performance is not negatively affected in the slm1 and the main difference with the wild type is an improved carbon partitioning towards TAG. PMID:24883102

2014-01-01

450

Movement of Tagged Red Snapper in the Northern Gulf of Mexico  

Microsoft Academic Search

A tagging study of adult red snapper Lutjanus campechanus was conducted in an area of artificial reefs in the northcentral Gulf of Mexico during March 1995 through August 1999. A total of 2,932 red snapper angled at nine artificial reef tagging sites were measured and tagged with internal anchor tags. Tagged fish were either released over their site of capture

William F. Patterson III; J. Carter Watterson; Robert L. Shipp; James H. Cowan Jr

2001-01-01

451

Management and Ecological Note Long-term anchor tag retention in yellow perch,  

E-print Network

Management and Ecological Note Long-term anchor tag retention in yellow perch, Perca flavescens, Perca flavescens, tag loss, tag retention, yellow perch. Tagging and marking techniques are frequently of yellow perch, Perca flavescens (Mitchill), information was needed on long-term tag retention

452

Passive Integrated Transponder Tag Retention Rates in Headwater Populations of Coastal Cutthroat Trout  

Microsoft Academic Search

Passive integrated transponder (PIT) tags have desirable qualities (e.g., unique identification, indefinite tag life, and capacity for remote detection) that make them useful for evaluating survival, growth, and movement of fish, but low tag retention rates can confound data interpretation. Although the effects of PIT tags on short-term growth and survival have been minimal and tag retention rates in laboratory

Douglas S. Bateman; Robert E. Gresswell; Aaron M. Berger

2009-01-01

453

Optimisation of Downscaled Tandem Affinity Purifications to Identify Core Protein Complexes.  

PubMed

In this study we show that via stable, retroviral-expression of tagged EGFR del (L747-S752 deletion mutant) in the PC9 lung cancer cell line and stable doxycycline-inducible expression of tagged Grb2 using a Flp-mediated recombination HEK293 cell system, the SH-TAP can be downscaled to 5 to 12.5 mg total protein input (equivalent to 0.5 - 1 × 15 cm culture plate or 4 - 8 × 10(6) cells). The major constituents of the EGFR del complex (USB3B, GRB2, ERRFI, HSP7C, GRP78, HSP71) and the Grb2 complex (ARHG5, SOS1, ARG35, CBL, CBLB, PTPRA, SOS2, DYN2, WIPF2, IRS4) were identified. Adjustment of the quantity of digested protein injected into the mass spectrometer reveals that optimisation is required as high quantities of material led to a decrease in protein sequence coverage and the loss of some interacting proteins. This investigation should aid other researchers in performing tandem affinity purifications in general, and in particular, from low quantities of input material. PMID:24077984

Haura, Eric B; Sacco, Roberto; Li, Jiannong; Müller, André C; Grebien, Florian; Superti-Furga, Giulio; Bennett, Keiryn L

2012-05-01

454

Targeted tandem affinity purification of PSD-95 recovers core postsynaptic complexes and schizophrenia susceptibility proteins  

PubMed Central

The molecular complexity of mammalian proteomes demands new methods for mapping the organization of multiprotein complexes. Here, we combine mouse genetics and proteomics to characterize synapse protein complexes and interaction networks. New tandem affinity purification (TAP) tags were fused to the carboxyl terminus of PSD-95 using gene targeting in mice. Homozygous mice showed no detectable abnormalities in PSD-95 expression, subcellular localization or synaptic electrophysiological function. Analysis of multiprotein complexes purified under native conditions by mass spectrometry defined known and new interactors: 118 proteins comprising crucial functional components of synapses, including glutamate receptors, K+ channels, scaffolding and signaling proteins, were recovered. Network clustering of protein interactions generated five connected clusters, with two clusters containing all the major ionotropic glutamate receptors and one cluster with voltage-dependent K+ channels. Annotation of clusters with human disease associations revealed that multiple disorders map to the network, with a significant correlation of schizophrenia within the glutamate receptor clusters. This targeted TAP tagging strategy is generally applicable to mammalian proteomics and systems biology approaches to disease. PMID:19455133

Fernández, Esperanza; Collins, Mark O; Uren, Rachel T; Kopanitsa, Maksym V; Komiyama, Noboru H; Croning, Mike D R; Zografos, Lysimachos; Armstrong, J Douglas; Choudhary, Jyoti S; Grant, Seth G N

2009-01-01

455

Peptide nucleic acid probe for protein affinity purification based on biotin-streptavidin interaction and peptide nucleic acid strand hybridization.  

PubMed

We describe a new method for protein affinity purification that capitalizes on the high affinity of streptavidin for biotin but does not require dissociation of the biotin-streptavidin complex for protein retrieval. Conventional reagents place both the selectively reacting group (the "warhead") and the biotin on the same molecule. We place the warhead and the biotin on separate molecules, each linked to a short strand of peptide nucleic acid (PNA), synthetic polymers that use the same bases as DNA but attached to a backbone that is resistant to attack by proteases and nucleases. As in DNA, PNA strands with complementary base sequences hybridize. In conditions that favor PNA duplex formation, the warhead strand (carrying the tagged protein) and the biotin strand form a complex that is held onto immobilized streptavidin. As in DNA, the PNA duplex dissociates at moderately elevated temperature; therefore, retrieval of the tagged protein is accomplished by a brief exposure to heat. Using iodoacetate as the warhead, 8-base PNA strands, biotin, and streptavidin-coated magnetic beads, we demonstrate retrieval of the cysteine protease papain. We were also able to use our iodoacetyl-PNA:PNA-biotin probe for retrieval and identification of a thiol reductase and a glutathione transferase from soybean seedling cotyledons. PMID:25447466

Tse, Jenny; Wang, Yuanyuan; Zengeya, Thomas; Rozners, Eriks; Tan-Wilson, Anna

2015-02-01

456

Conjugate connections and Radon's theorem in affine differential geometry  

Microsoft Academic Search

For a given nondegenerate hypersurfaceMn in affine space Rn+1 there exist an affine connection ?, called the induced connection, and a nondegenerate metrich, called the affine metric, which are uniquely determined. The cubic formC=?h is totally symmetric and satisfies the so-called apolarity condition relative toh. A natural question is, conversely, given an affine connection ? and a nondegenerate metrich on

Franki Dillen; Katsumi Nomizu; Luc Vranken

1990-01-01

457

Asymptotic representations of quantum affine superalgebras  

E-print Network

We study representations of the $q$-Yangian, the upper Borel subalgebra with respect to RTT realization of the quantum affine superalgebra associated with the Lie superalgebra $\\mathfrak{gl}(M,N)$. Following the work of Hernandez-Jimbo, we construct inductive systems of Kirillov-Reshetikhin modules by using a cyclicity result of tensor products of these modules we established recently, and realize their inductive limits as modules over the $q$-Yangian, extending the asymptotic construction of Hernandez-Jimbo to the super case. Then, we propose a new asymptotic construction on the inductive limits of the same inductive systems, resulting in modules over the full quantum affine superalgebra depending on an additional parameter. $q$-character and Gelfand-Tsetlin basis for these two kinds of modules are also investigated.

Huafeng Zhang

2014-10-03

458

Smooth big bounce from affine quantization  

NASA Astrophysics Data System (ADS)

We examine the possibility of dealing with gravitational singularities on a quantum level through the use of coherent state or wavelet quantization instead of canonical quantization. We consider the Robertson-Walker metric coupled to a perfect fluid. It is the simplest model of a gravitational collapse, and the results obtained here may serve as a useful starting point for more complex investigations in the future. We follow a quantization procedure based on affine coherent states or wavelets built from the unitary irreducible representation of the affine group of the real line with positive dilation. The main issue of our approach is the appearance of a quantum centrifugal potential allowing for regularization of the singularity, essential self-adjointness of the Hamiltonian, and unambiguous quantum dynamical evolution.

Bergeron, Hervé; Dapor, Andrea; Gazeau, Jean Pierre; Ma?kiewicz, Przemys?aw

2014-04-01

459

Affinity Chromatography in Nonionic Detergent Solutions  

NASA Astrophysics Data System (ADS)

Anionic dye affinity chromatography is commonly unproductive in the presence of nonionic detergents used to extract particulate proteins. Using lactate dehydrogenase as a model protein, Cibacron blue F3GA as a model dye, and Triton X-100 as a model detergent, we find that the dye is encapsulated in nonionic detergent micelles, rendering the dye incapable of ligation with the enzyme. However, the dye can be liberated from the micelles without altering the nonionic detergent concentration by addition of an anionic detergent, such as deoxycholate or sodium dodecyl sulfate, forming mixed anionic/nonionic micelles that displace the anionic dye. Encapsulation of the anionic detergents prevents their activity as protein denaturants. These observations have been successfully translated to the dye affinity chromatography of a detergent extract of brain particulate cyclic nucleotide phosphodiesterase.

Robinson, Jack B.; Strottmann, James M.; Wick, Donald G.; Stellwagen, Earle

1980-10-01

460

Affinity chromatography in nonionic detergent solutions.  

PubMed Central

Anionic dye affinity chromatography is commonly unproductive in the presence of nonionic detergents used to extract particulate proteins. Using lactate dehydrogenase as a model protein, Cibacron blue F3GA as a model dye, and Triton X-100 as a model detergent, we find that the dye is encapsulated in nonionic detergent micelles, rendering the dye incapable of ligation with the enzyme. However, the dye can be liberatd from the micelles without altering the nonionic detergent concentration by addition of an anionic detergent, such as deoxycholate or sodium dodecyl sulfate, forming mixed anionic/nonionic micelles that displace the anionic dye. Encapsulation of the anionic detergents prevents their activity as protein denaturants. These observations have been successfuly translated to the dye affinity chromatography of a detergent extract of brain particulate cyclic nucleotide phosphodiesterase. PMID:6934517

Robinson, J B; Strottmann, J M; Wick, D G; Stellwagen, E

1980-01-01

461

Avoiding degenerate coframes in an affine gauge approach to quantum gravity  

SciTech Connect

This report discusses the following concepts on quantum gravity: The affine gauge approach; affine gauge transformations versus active differomorphisms; affine gauge approach to quantum gravity with topology change.

Mielke, E.W.; McCrea, J.D.; Ne`eman, Y.; Hehl, F.W.

1993-04-01

462

Automata and cells in affine Weyl groups  

Microsoft Academic Search

Let W~ be an affine Weyl group, and let C be a left, right, or two-sided\\u000aKazhdan--Lusztig cell in W~. Let Reduced (C) be the set of all reduced\\u000aexpressions of elements of C, regarded as a formal language in the sense of the\\u000atheory of computation. We show that Reduced (C) is a regular language. Hence\\u000athe reduced expressions

Paul E. Gunnells

2008-01-01

463

On Automorphisms of the Affine Cremona Group  

E-print Network

We show that every automorphism of the group $\\mathcal{G}_n:= \\textrm{Aut}(\\mathbb{A}^n)$ of polynomial automorphisms of complex affine $n$-space $\\mathbb{A}^n=\\mathbb{C}^n$ is inner up to field automorphisms when restricted to the subgroup $T \\mathcal{G}_n$ of tame automorphisms. This generalizes a result of \\textsc{Julie Deserti} who proved this in dimension $n=2$ where all automorphisms are tame: $T \\mathcal{G}_2 = \\mathcal{G}_2$.

Kraft, Hanspeter

2011-01-01

464

Discovering High-Affinity Ligands for Proteins  

NSDL National Science Digital Library

This article reports on development of a method for producing high-affinity ligands in which small molecules that bind to proximal subsites of a protein are identified in an NMR-based screen and then linked together in their experimentally determined bound orientations. The method is called â??SAR by NMR,â? which stands for â??structure-activity relationships by nuclear magnetic resonance.â?

Philip J Hajduk (Abbott Laboratories; )

1997-10-17

465

On quiver varieties and affine Grassmannians of type A  

Microsoft Academic Search

We construct Nakajima's quiver varieties of type A in terms of affine Grassmannians of type A. This gives a compactification of quiver varieties and a decomposition of affine Grassmannians into a disjoint union of quiver varieties. Consequently, singularities of quiver varieties, nilpotent orbits and affine Grassmannians are the same in type A. The construction also provides a geometric framework for

Ivan Mirkovi?; Maxim Vybornov

2003-01-01

466

On quiver varieties and affine Grassmannians of type A  

Microsoft Academic Search

We construct Nakajima's quiver varieties of type A in terms of affine Grassmannians of type A. This gives a compactification of quiver varieties and a decomposition of affine Grassmannians into a disjoint union of quiver varieties. Consequently, singularities of quiver varieties, nilpotent orbits and affine Grassmannians are the same in type A. The construction also provides a geometric framework for

Ivan Mirkovic; Maxim Vybornov

2002-01-01

467

Metal chelate affinity chromatography, a new approach to protein fractionation  

Microsoft Academic Search

CONVENTIONAL nonspecific precipitation methods sometimes depend on affinities which can be used in a more selective fashion by modern chromatographic techniques. The affinity of proteins for heavy metal ions, for example, may provide a basis for their purification and analysis. A highly flexible method based on such affinities is described here.

Jerker Porath; Jan Carlsson; Ingmar Olsson; Greta Belfrage

1975-01-01

468

On Affine Invariant Clustering and Automatic Cast Listing in Movies  

Microsoft Academic Search

We develop a distance metric for clustering and classification algo- rithms which is invariant to affine transformations and includes priors on the transformation parameters. Such clustering requirements are generic to a num- ber of problems in computer vision. We extend existing techniques for affine-invariant clustering, and show that the new distance metric outperforms existing approximations to affine invariant dis- tance

Andrew W. Fitzgibbon; Andrew Zisserman

2002-01-01

469

university-logo Affine space forms and hyperbolic geometry  

E-print Network

University of Singapore #12;university-logo Affine space forms and hyperbolic geometry Euclidean manifolds. #12;university-logo Affine space forms and hyperbolic geometry Euclidean manifolds When can a group G-logo Affine space forms and hyperbolic geometry Euclidean manifolds When can a group G act on Rn with quotient

Goldman, William

470

Divergence Analysis with Affine Constraints Diogo Sampaio Rafael Martins  

E-print Network

as affine functions of thread iden- tifiers. We have implemented our divergence analysis with affineDivergence Analysis with Affine Constraints Diogo Sampaio Rafael Martins Fernando Magno Quint, which classify variables as uniform, if they have the same value on every thread, or divergent

Paris-Sud XI, Université de

471

Notch and Integrin Affinity: A Sticky Situation  

NSDL National Science Digital Library

The Notch pathway is a conserved signal transduction system that mediates intercellular signaling to regulate cell fate decisions in various tissues. Dysregulation of Notch activity results in various disorders, including cardiovascular diseases and cancer. Notch regulates cell fate through a number of mechanisms that include control of cell proliferation, survival, migration, and differentiation. Notch activation increases vascular endothelial cell adhesion through the enhancement of ?1 integrin affinity for fibronectin, collagens I and IV, and vitronectin without altering the abundance of ?1 integrin at the cell surface. A study now suggests that this Notch-dependent increase in ?1 integrin affinity occurs through the activation of the small guanosine triphosphate (GTP)–binding protein, R-Ras. It is proposed that Notch-dependent activation of R-Ras reverses H-Ras–mediated suppression of integrin affinity. Activation of R-Ras by Notch may be triggered by a noncanonical CSL (CBF1 or RBP-J? in vertebrates, Suppressor of Hairless in Drosophila, Lag-1 in Caenorhabditis elegans)–independent pathway. Because R-Ras is selectively distributed in vascular cells, these findings are of particular importance in understanding the effector functions of Notch in the vascular system.

Aly Karsan (Vancouver; British Columbia Cancer Agency and University of British Columbia REV)

2008-01-15

472

A MEMS Dielectric Affinity Glucose Biosensor  

PubMed Central

Continuous glucose monitoring (CGM) sensors based on affinity detection are desirable for long-term and stable glucose management. However, most affinity sensors contain mechanical moving structures and complex design in sensor actuation and signal readout, limiting their reliability in subcutaneously implantable glucose detection. We have previously demonstrated a proof-of-concept dielectric glucose sensor that measured pre-mixed glucose-sensitive polymer solutions at various glucose concentrations. This sensor features simplicity in sensor design, and possesses high specificity and accuracy in glucose detection. However, lack of glucose diffusion passage, this device is unable to fulfill real-time in-vivo monitoring. As a major improvement to this device, we present in this paper a fully implantable MEMS dielectric affinity glucose biosensor that contains a perforated electrode embedded in a suspended diaphragm. This capacitive-based sensor contains no moving parts, and enables glucose diffusion and real-time monitoring. The experimental results indicate that this sensor can detect glucose solutions at physiological concentrations and possesses good reversibility and reliability. This sensor has a time constant to glucose concentration change at approximately 3 min, which is comparable to commercial systems. The sensor has potential applications in fully implantable CGM that require excellent long-term stability and reliability. PMID:24511215

Huang, Xian; Li, Siqi; Davis, Erin; Li, Dachao; Wang, Qian; Lin, Qiao

2013-01-01

473

Conversion of low-affinity peptides to high-affinity peptide binders by using a ?-hairpin scaffold-assisted approach.  

PubMed

Affinity maturation of protein-targeting peptides is generally accomplished by homo- or heterodimerization of known peptides. However, applying a heterodimerization approach is difficult because it is not clear a priori what length or type of linker is required for cooperative binding to a target. Thus, an efficient and simple affinity maturation method for converting low-affinity peptides into high-affinity peptides would clearly be advantageous for advancing peptide-based therapeutics. Here, we describe the development of a novel affinity maturation method based on a robust ?-hairpin scaffold and combinatorial phage-display technology. With this strategy, we were able to increase the affinity of existing peptides by more than four orders of magnitude. Taken together, our data demonstrate that this scaffold-assisted approach is highly efficient and effective in generating high-affinity peptides from their low-affinity counterparts. PMID:25371172

Kim, Sunghyun; Kim, Daejin; Lee, Yonghyun; Jeon, Hyungsu; Lee, Byung-Heon; Jon, Sangyong

2015-01-01

474

Engineering of Bispecific Affinity Proteins with High Affinity for ERBB2 and Adaptable Binding to Albumin  

PubMed Central

The epidermal growth factor receptor 2, ERBB2, is a well-validated target for cancer diagnostics and therapy. Recent studies suggest that the over-expression of this receptor in various cancers might also be exploited for antibody-based payload delivery, e.g. antibody drug conjugates. In such strategies, the full-length antibody format is probably not required for therapeutic effect and smaller tumor-specific affinity proteins might be an alternative. However, small proteins and peptides generally suffer from fast excretion through the kidneys, and thereby require frequent administration in order to maintain a therapeutic concentration. In an attempt aimed at combining ERBB2-targeting with antibody-like pharmacokinetic properties in a small protein format, we have engineered bispecific ERBB2-binding proteins that are based on a small albumin-binding domain. Phage display selection against ERBB2 was used for identification of a lead candidate, followed by affinity maturation using second-generation libraries. Cell surface display and flow-cytometric sorting allowed stringent selection of top candidates from pools pre-enriched by phage display. Several affinity-matured molecules were shown to bind human ERBB2 with sub-nanomolar affinity while retaining the interaction with human serum albumin. Moreover, parallel selections against ERBB2 in the presence of human serum albumin identified several amino acid substitutions that dramatically modulate the albumin affinity, which could provide a convenient means to control the pharmacokinetics. The new affinity proteins competed for ERBB2-binding with the monoclonal antibody trastuzumab and recognized the native receptor on a human cancer cell line. Hence, high affinity tumor targeting and tunable albumin binding were combined in one small adaptable protein. PMID:25089830

Nilvebrant, Johan; Åstrand, Mikael; Georgieva-Kotseva, Maria; Björnmalm, Mattias; Löfblom, John; Hober, Sophia

2014-01-01

475

Effect of Passive Integrated Transponder Tag Implantation Site on Tag Retention, Growth, and Survival of Two Sizes of Juvenile Bluegills and Yellow Perch  

Microsoft Academic Search

Passive integrated transponder (PIT) tags are commonly used to monitor growth, habitat use, activity rates, and survival of individual fish. However, for successful completion of research objectives, the tags must be retained and must not affect fish growth or survival. We compared the effects of PIT tagging location on tag retention, growth, and survival of juvenile bluegills Lepomis macrochirus and

Mark A. Kaemingk; Michael J. Weber; Paul R. McKenna; Michael L. Brown

2011-01-01

476

HIP-tags architecture implementation for the Internet of things  

Microsoft Academic Search

This paper describes a possible implementation for the innovative and highly secure networking architecture dedicated to the Internet of Things (IoT). We propose an infrastructure that works with a new type of tags, supporting the upcoming standard Host Identity Protocol (HIP). Our main concern is to ensure RFID tags privacy, while enabling things to things communications.

Pascal Urien; Simon Elrharbi; Dorice Nyamy; Hervé Chabanne; Thomas Icart; François Lecocq; Cyrille Pépin; Khalifa Toumi; Mathieu Bouet; Guy Pujolle; Patrice Krzanik; Jean-Ferdinand Susini

2009-01-01

477

MFR PAPER 1007 Sonic Tags in Sockeye Salmon,  

E-print Network

rate of fis h were determined b) mean of a sonic tag placed in the stomach. The tag had a high-frequency the water of a metropolitan area. Their travel route lay between Puget Sound and Lake Washington. a distance- mates placed the returns at approxi- mately 250.000 fish . To enter the lake fro m Puget Sound. fish

478

Tagging fast neutrons from an (241)Am/(9)Be source.  

PubMed

Shielding, coincidence, and time-of-flight measurement techniques are employed to tag fast neutrons emitted from an (241)Am/(9)Be source resulting in a continuous polychromatic energy-tagged beam of neutrons with energies up to 7MeV. The measured energy structure of the beam agrees qualitatively with both previous measurements and theoretical calculations. PMID:25644080

Scherzinger, J; Annand, J R M; Davatz, G; Fissum, K G; Gendotti, U; Hall-Wilton, R; Håkansson, E; Jebali, R; Kanaki, K; Lundin, M; Nilsson, B; Rosborge, A; Svensson, H

2015-04-01

479

Covalent Tagging of Phosphorylated Peptides By Phosphate-Specific Deoxyribozymes  

PubMed Central

Phosphopeptides tagged: Phosphorylated tyrosine and serine residues in peptides are modified selectively by DNA catalysts (see the figure). The deoxyribozymes catalyze covalent attachment of an RNA tag to a range of peptide sequences, establishing proof-of-principle for a new approach to phosphopeptide analysis. PMID:22315198

Sachdeva, Amit; Chandra, Madhavaiah; Chandrasekar, Jagadeeswaran; Silverman, Scott K.

2012-01-01

480

Acoustic competition in the gulf toadfish Opsanus beta: Acoustic tagging  

NASA Astrophysics Data System (ADS)

Nesting male gulf toadfish Opsanus beta produce a boatwhistle advertisement call used in male-male competition and to attract females and an agonistic grunt call. The grunt is a short-duration pulsatile call, and the boatwhistle is a complex call typically consisting of zero to three introductory grunts, a long tonal boop note, and zero to three shorter boops. The beginning of the boop note is also gruntlike. Anomalous boatwhistles contain a short-duration grunt embedded in the tonal portion of the boop or between an introductory grunt and the boop. Embedded grunts have sound-pressure levels and frequency spectra that correspond with those of recognized neighbors, suggesting that one fish is grunting during another's call, a phenomenon here termed acoustic tagging. Snaps of nearby pistol shrimp may also be tagged, and chains of tags involving more than two fish occur. The stimulus to tag is a relatively intense sound with a rapid rise time, and tags are generally produced within 100 ms of a trigger stimulus. Time between the trigger and the tag decreases with increased trigger amplitude. Tagging is distinct from increased calling in response to natural calls or stimulatory playbacks since calls rarely overlap other calls or playbacks. Tagging is not generally reciprocal between fish, suggesting parallels to dominance displays.

Thorson, Robert F.; Fine, Michael L.

2002-05-01

481

PAP: A privacy and authentication protocol for passive RFID tags  

Microsoft Academic Search

Passive Radio Frequency Identification (RFID) tags, due to their ability to uniquely identify every individual item and low cost, are well suited for supply chain management and are expected to replace barcodes in the near future. However, unlike barcodes, these tags have a longer range in which they are allowed to be scanned, subjecting them to unauthorized scanning by malicious

Alex X. Liu; LeRoy A. Bailey

2009-01-01

482

NE Pacific Basin --Tagging Data Kate Myers, Ph.D.  

E-print Network

Ocean B: NE Pacific Basin --Tagging Data Kate Myers, Ph.D. Principal Investigator, High Seas Salmon ocean tagging research on Columbia River salmon and steelhead migrating in the NE Pacific Basin. The following premises are central to science policy implications of what we have learned from NE Pacific Basin

483

Results from Plantings of Tagged Trout in Spring Creek, Pennsylvania  

Microsoft Academic Search

Data on anglers' catches, the growth and migration of stocked trout, and the efficiency of fall and spring plantings were obtained by means of a creel census in Spring Creek, Pennsylvania, during 1939. Of 2,130 tagged trout planted, 50.8 per cent were recovered by anglers. Due to heavy fishing pressure, more than 40 per cent of all tagged trout taken

Gordon L. Trembley

1945-01-01

484

Preparing Research Boats to Track Tagged Pallid Sturgeon  

USGS Multimedia Gallery

Biologist, Dave Combs prepares a tracking boat (foreground) and a DIDSON survey boat (background) to search the Yellowstone River for tagged pallid sturgeon, Near Fairview, Montana.  Pallid sturgeon in Montana are tagged with radio telemetry transmitters that are detected with large antennas mo...