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Sample records for japanese flowering cherry

  1. Interaction between Japanese flowering cherry trees and some wild animals observed during physiological experiment in fields

    NASA Technical Reports Server (NTRS)

    Nakamura, Teruko

    2003-01-01

    We have studied the weeping habit of Japanese flowering cherry tree in the field of Tama Forest Science Garden, Forestry and Forest Products Research Institute at the foot of Mt. Takao. Since cherry trees at various age were the materials for our plant physiology experiments, our studies were conducted in the fields where we experienced certain difficulties. Even under such difficult environment that was rather unexpected and uncontrollable, we could obtain fruitful results on the growth of cherry tree, and found them scientifically significant, especially in terms of biological effects of gravity on earth. Moreover, a lot of interesting interactions of cherry trees with various kinds of animals were observed in parallel to the plant physiology.

  2. Japanese flowering cherry tree as a woody plant candidate grown in space

    NASA Astrophysics Data System (ADS)

    Tomita-Yokotani, K.; Yoshida, S.; Hashimoto, H.; Nyunoya, H.; Funada, R.; Katayama, T.; Suzuki, T.; Honma, T.; Nagatomo, M.; Nakamura, T.

    We are proposing to raise woody plant in space for several applications Japanese flowering cherry tree is a candidate to do wood science in space Mechanism of sensing gravity and controlling shape of tree has been studied quite extensively Cherry mutants associated with gravity are telling responsible plant hormones and molecular machinery for plant adaptation against action of gravity Space experiment using our wood model contribute to understand molecular and cellular process of gravitropism in plant Tree is considered to be an important member in space agriculture to produce excess oxygen wooden materials for constructing living environment and provide biomass for cultivating mushrooms and insects Furthermore trees and their flowers improve quality of life under stressful environment in outer space

  3. Growth and photosynthesis of Japanese flowering cherry under simulated microgravity conditions

    NASA Technical Reports Server (NTRS)

    Sugano, Mami; Ino, Yoshio; Nakamura, Teruko

    2002-01-01

    The photosynthetic rate, the leaf characteristics related to photosynthesis, such as the chlorophyll content, chlorophyll a/b ratio and density of the stomata, the leaf area and the dry weight in seedlings of Japanese flowering cherry grown under normal gravity and simulated microgravity conditions were examined. No significant differences were found in the photosynthetic rates between the two conditions. Moreover, leaf characteristics such as the chlorophyll content, chlorophyll a/b ratio and density of the stomata in the seedlings grown under the simulated microgravity condition were not affected. However, the photosynthetic product of the whole seedling under the simulated microgravity condition increased compared with the control due to its leaf area increase. The results suggest that dynamic gravitational stimulus controls the partitioning of the products of photosynthesis.

  4. Interspecific hybridizations in ornamental flowering cherries (Prunus species)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Flowering cherries belong to the genus Prunus L., consisting primarily of species native to Asia. Despite the popularity of ornamental cherry trees in the landscape, most ornamental Prunus planted in the U.S. are derived from a limited genetic base of Japanese flowering cherry taxa. Controlled cross...

  5. Expression of gibberellin 3 beta-hydroxylase gene in a gravi-response mutant, weeping Japanese flowering cherry

    NASA Technical Reports Server (NTRS)

    Sugano, Mami; Nakagawa, Yuriko; Nyunoya, Hiroshi; Nakamura, Teruko

    2004-01-01

    Expressions of the gibberellin biosynthesis gene were investigated in a normal upright type and a gravi-response mutant, a weeping type of Japanese flowering cherry (Prunus spachiana), that is unable to support its own weight and elongates downward. A segment of the gibberellin 3 beta-hydroxylase cDNA of Prunus spachiana (Ps3ox), which is responsible for active gibberellin synthesis, was amplified by using real-time RT-PCR. The content of Ps3ox mRNA in the weeping type was much greater than that in the upright type, while the endogenous gibberellin level was much higher in the elongating zone of the weeping type. These results suggest that the amount and distribution of synthesized gibberellin regulate secondary xylem formation, and the unbalanced distribution of gibberellin affects the gravi-response of the Prunus tree.

  6. Evaluation of flowering cherry (Prunus L.) species, hybrids, and cultivars using SSR markers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Flowering cherries belong to the genus Prunus L., consisting primarily of species native to Asia. Despite the popularity of ornamental cherry trees in the landscape, most ornamental Prunus planted in the U.S. are derived from a limited genetic base of Japanese flowering cherry taxa. A diverse collec...

  7. 'DREAM CATCHER' AND 'FIRST LADY' FLOWERING CHERRY

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ornamental flowering cherry trees (Prunus L. species) are popular landscape plants, made famous in the U.S. by the historic Tidal Basin cherries planted in Washington, D.C. Although planted primarily for their spring bloom, flowering cherries are also used as street or shade trees, and are valued fo...

  8. Characterization of a Flowering Cherry Strain of Cherry Necrotic Rusty Mottle Virus usty

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The host range and complete nucleotide sequences of two Cherry necrotic rusty mottle virus (CNRMV) isolates (FC4 and FC5) infecting flowering cherry accessions imported from Japan are described. Of the plants tested, sweet cherry cvs. ‘Bing’, ‘Canindex’, ‘Mazzard’ and ‘Sam’, flowering cherry cv. ‘Kw...

  9. Flowering of Japanese astronomy

    SciTech Connect

    Kozai, Y.

    1988-06-01

    A development history is presented for Japanese astronomy from the 6th century to the present day, together with a status report and account of future plans. About 500 professionals currently belong to the Astronomical Society of Japan. Tokyo's Mitaka Observatory employs a staff of about 70 astronomers; most modern astronomical instruments, however, have been installed at sites outside the Tokyo area. The limitations of present instruments are notably severe for astronomers working in the visible and IR wavelengths.

  10. 'Helen Taft' and 'Abigail Adams' flowering cherries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The U.S. National Arboretum has an ongoing breeding program aimed at broadening the genetic base of ornamental cherry trees (Prunus species) by developing new cultivars with disease and pest resistance, tolerance to environmental stresses, and superior ornamental characteristics. This report documen...

  11. Mapping of Candidate Genes Involved in Bud Dormancy and Flowering Time in Sweet Cherry (Prunus avium)

    PubMed Central

    Le Dantec, Loïck; Quero-García, José; Barreneche, Teresa; Wenden, Bénédicte; Dirlewanger, Elisabeth

    2015-01-01

    The timing of flowering in perennial plants is crucial for their survival in temperate climates and is regulated by the duration of bud dormancy. Bud dormancy release and bud break depend on the perception of cumulative chilling during endodormancy and heat during the bud development. The objectives of this work were to identify candidate genes involved in dormancy and flowering processes in sweet cherry, their mapping in two mapping progenies ‘Regina’ × ‘Garnet’ and ‘Regina’ × ‘Lapins’, and to select those candidate genes which co-localized with quantitative trait loci (QTLs) associated with temperature requirements for bud dormancy release and flowering. Based on available data on flowering processes in various species, a list of 79 candidate genes was established. The peach and sweet cherry orthologs were identified and primers were designed to amplify sweet cherry candidate gene fragments. Based on the amplified sequences of the three parents of the mapping progenies, SNPs segregations in the progenies were identified. Thirty five candidate genes were genetically mapped in at least one of the two progenies and all were in silico mapped. Co-localization between candidate genes and QTLs associated with temperature requirements and flowering date were identified for the first time in sweet cherry. The allelic composition of the candidate genes located in the major QTL for heat requirements and flowering date located on linkage group 4 have a significant effect on these two traits indicating their potential use for breeding programs in sweet cherry to select new varieties adapted to putative future climatic conditions. PMID:26587668

  12. Mapping of Candidate Genes Involved in Bud Dormancy and Flowering Time in Sweet Cherry (Prunus avium).

    PubMed

    Castède, Sophie; Campoy, José Antonio; Le Dantec, Loïck; Quero-García, José; Barreneche, Teresa; Wenden, Bénédicte; Dirlewanger, Elisabeth

    2015-01-01

    The timing of flowering in perennial plants is crucial for their survival in temperate climates and is regulated by the duration of bud dormancy. Bud dormancy release and bud break depend on the perception of cumulative chilling during endodormancy and heat during the bud development. The objectives of this work were to identify candidate genes involved in dormancy and flowering processes in sweet cherry, their mapping in two mapping progenies 'Regina' × 'Garnet' and 'Regina' × 'Lapins', and to select those candidate genes which co-localized with quantitative trait loci (QTLs) associated with temperature requirements for bud dormancy release and flowering. Based on available data on flowering processes in various species, a list of 79 candidate genes was established. The peach and sweet cherry orthologs were identified and primers were designed to amplify sweet cherry candidate gene fragments. Based on the amplified sequences of the three parents of the mapping progenies, SNPs segregations in the progenies were identified. Thirty five candidate genes were genetically mapped in at least one of the two progenies and all were in silico mapped. Co-localization between candidate genes and QTLs associated with temperature requirements and flowering date were identified for the first time in sweet cherry. The allelic composition of the candidate genes located in the major QTL for heat requirements and flowering date located on linkage group 4 have a significant effect on these two traits indicating their potential use for breeding programs in sweet cherry to select new varieties adapted to putative future climatic conditions. PMID:26587668

  13. RNA-Seq-based transcriptome analysis of dormant flower buds of Chinese cherry (Prunus pseudocerasus).

    PubMed

    Zhu, Youyin; Li, Yongqiang; Xin, Dedong; Chen, Wenrong; Shao, Xu; Wang, Yue; Guo, Weidong

    2015-01-25

    Bud dormancy is a critical biological process allowing Chinese cherry (Prunus pseudocerasus) to survive in winter. Due to the lake of genomic information, molecular mechanisms triggering endodormancy release in flower buds have remained unclear. Hence, we used Illumina RNA-Seq technology to carry out de novo transcriptome assembly and digital gene expression profiling of flower buds. Approximately 47million clean reads were assembled into 50,604 sequences with an average length of 837bp. A total of 37,650 unigene sequences were successfully annotated. 128 pathways were annotated by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and metabolic, biosynthesis of second metabolite and plant hormone signal transduction accounted for higher percentage in flower bud. In critical period of endodormancy release, 1644, significantly differentially expressed genes (DEGs) were identified from expression profile. DEGs related to oxidoreductase activity were especially abundant in Gene Ontology (GO) molecular function category. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis demonstrated that DEGs were involved in various metabolic processes, including phytohormone metabolism. Quantitative real-time PCR (qRT-PCR) analysis indicated that levels of DEGs for abscisic acid and gibberellin biosynthesis decreased while the abundance of DEGs encoding their degradation enzymes increased and GID1 was down-regulated. Concomitant with endodormancy release, MADS-box transcription factors including P. pseudocerasus dormancy-associated MADS-box (PpcDAM), Agamous-like2, and APETALA3-like genes, shown remarkably epigenetic roles. The newly generated transcriptome and gene expression profiling data provide valuable genetic information for revealing transcriptomic variation during bud dormancy in Chinese cherry. The uncovered data should be useful for future studies of bud dormancy in Prunus fruit trees lacking genomic information. PMID:25447903

  14. Evaluation of the virus and viroid infection status of flowering cherry (Prunus yedoensis) collections in Korea and the United States

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The virus and viroid infection status of flowering cherry trees (Prunus yedoensis) in prominent ornamental collections in Korea (Seoul, Jinhae, Jeju) and the U.S. (Washington, D.C.) was investigated. A total of 344 trees were tested by conventional RT-PCR for 13 viruses and 2 viroids. Eight viruses ...

  15. The phenology of cherry blossom (Prunus yedoensis ``Somei-yoshino'') and the geographic features contributing to its flowering

    NASA Astrophysics Data System (ADS)

    Ohashi, Yukitaka; Kawakami, Hiroshi; Shigeta, Yoshinori; Ikeda, Hiroshi; Yamamoto, Nobuko

    2012-09-01

    We investigated relationships between the flowering phenology of Prunus yedoensis "Somei-yoshino" (cherry blossom) and the local temperatures in Japan. Our observations were carried out across the Okayama Plain, which included Okayama City (about 700,000 inhabitants), from the winter of 2008 to the spring of 2009. Local air temperature (AT) and the globe temperature (GT) were recorded at the tree height. The flowering dates (FDs) of P. yedoensis were earliest in the central commercial area (located at the center of the plain), followed by the north residential area (further inland), and finally the south residential area (seaward). The recorded FDs were related to the period-averaged daily maximum/minimum AT and GT, and the phenologically effective AT and GT defined in this study. Of these parameters, the phenologically effective GTs correlated most with the FDs. Since the GT is determined by AT, solar and infrared radiations, and wind speed, our previous result suggests that a combination of these three components surrounding the tree is more important for budding and flowering than is AT alone. The supposition is supported by the flowering of P. yedoensis being the latest at the coastal region of the Okayama Plain where the AT were higher than at the inland region, excluding the urban area; it is probably caused by stronger winds there than at the other sites.

  16. The phenology of cherry blossom (Prunus yedoensis "Somei-yoshino") and the geographic features contributing to its flowering.

    PubMed

    Ohashi, Yukitaka; Kawakami, Hiroshi; Shigeta, Yoshinori; Ikeda, Hiroshi; Yamamoto, Nobuko

    2012-09-01

    We investigated relationships between the flowering phenology of Prunus yedoensis "Somei-yoshino" (cherry blossom) and the local temperatures in Japan. Our observations were carried out across the Okayama Plain, which included Okayama City (about 700,000 inhabitants), from the winter of 2008 to the spring of 2009. Local air temperature (AT) and the globe temperature (GT) were recorded at the tree height. The flowering dates (FDs) of P. yedoensis were earliest in the central commercial area (located at the center of the plain), followed by the north residential area (further inland), and finally the south residential area (seaward). The recorded FDs were related to the period-averaged daily maximum/minimum AT and GT, and the phenologically effective AT and GT defined in this study. Of these parameters, the phenologically effective GTs correlated most with the FDs. Since the GT is determined by AT, solar and infrared radiations, and wind speed, our previous result suggests that a combination of these three components surrounding the tree is more important for budding and flowering than is AT alone. The supposition is supported by the flowering of P. yedoensis being the latest at the coastal region of the Okayama Plain where the AT were higher than at the inland region, excluding the urban area; it is probably caused by stronger winds there than at the other sites. PMID:21953217

  17. A Rare Excitatory Amino Acid from Flowers of Zonal Geranium responsible for Paralyzing the Japanese Beetle

    Technology Transfer Automated Retrieval System (TEKTRAN)

    e Japanese beetle (Popillia japonica) exhibits rapid paralysis after consuming flowers from zonal geranium (Pelargonium × hortorum). Activity-guided fractionations were conducted with polar flower petal extracts from Pelargonium × hortorum cv. Nittany Lion Red, which led to the isolation of a paraly...

  18. Complete chloroplast genome of Prunus yedoensis Matsum.(Rosaceae), wild and endemic flowering cherry on Jeju Island, Korea.

    PubMed

    Cho, Myong-Suk; Hyun Cho, Chung; Yeon Kim, Su; Su Yoon, Hwan; Kim, Seung-Chul

    2016-09-01

    The complete chloroplast genome sequences of the wild flowering cherry, Prunus yedoensis Matsum., which is native and endemic to Jeju Island, Korea, is reported in this study. The genome size is 157 786 bp in length with 36.7% GC content, which is composed of LSC region of 85 908 bp, SSC region of 19 120 bp and two IR copies of 26 379 bp each. The cp genome contains 131 genes, including 86 coding genes, 8 rRNA genes and 37 tRNA genes. The maximum likelihood analysis was conducted to verify a phylogenetic position of the newly sequenced cp genome of P. yedoensis using 11 representatives of complete cp genome sequences within the family Rosaceae. The genus Prunus exhibited monophyly and the result of the phylogenetic relationship agreed with the previous phylogenetic analyses within Rosaceae. PMID:26329800

  19. Over-expression of the PaAP1 gene from sweet cherry (Prunus avium L.) causes early flowering in Arabidopsis thaliana.

    PubMed

    Wang, Jing; Zhang, Xiaoming; Yan, Guohua; Zhou, Yu; Zhang, Kaichun

    2013-02-15

    A homologue of SQUAMOSA/APETALA1, designated PaAP1, was isolated from Prunus avium by reverse transcription-PCR (RT-PCR). The full length of PaAP1 cDNA is 753 bp, and it codes for a polypeptide of 250 amino acid residues. Sequence comparison revealed that PaAP1 belongs to the MADS-box gene family. Phylogenetic analysis indicated that PaAP1 shared the highest identity with SQUA/AP1 homologues from Prunus serrulata. Real-time fluorescence quantitative PCR analysis showed that PaAP1 was expressed at high levels in petal, sepal, style, and flower buds, which was slightly different from the expression pattern of AP1 of Arabidopsis thaliana. To characterize the functions of PaAP1, we assessed Arabidopsis transformed with 35S::PaAP1. A total of 8 transgenic T(1) lines with an early flowering phenotype were obtained, and a 3:1 segregation ratio of flowering time was observed in the T(2) generation of 4 lines. This study provides the first functional analysis of an SQUA/AP1 homolog from P. avium and suggests that PaAP1 is potentially useful for shortening the juvenile period in sweet cherry. PMID:23206932

  20. Interploid hybridizations in ornamental cherries using Prunus maackii

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The United States National Arboretum has an ongoing flowering cherry (Prunus) breeding program aimed at broadening the genetic base of cultivated ornamental cherries by developing new cultivars with disease and pest resistance, tolerance to environmental stresses, and superior ornamental characteris...

  1. Metabolic changes upon flower bud break in Japanese apricot are enhanced by exogenous GA4.

    PubMed

    Zhuang, Weibing; Gao, Zhihong; Wen, Luhua; Huo, Ximei; Cai, Binhua; Zhang, Zhen

    2015-01-01

    Gibberellin (GA4) has a significant effect on promoting dormancy release in flower buds of Japanese apricot (Prunus mume Sieb. et Zucc). The transcriptomic and proteomic changes that occur after GA4 treatment have been reported previously; however, the metabolic changes brought about by GA4 remain unknown. The present study was undertaken to assess changes in metabolites in response to GA4 treatment, as determined using gas chromatography-mass spectrometry and principal component analysis. Fifty-five metabolites that exhibited more than two-fold differences in abundance (P < 0.05) between samples collected over time after a given treatment or between samples exposed to different treatments were studied further. These metabolites were categorized into six main groups: amino acids and their isoforms (10), amino acid derivatives (7), sugars and polyols (14), organic acids (12), fatty acids (4), and others (8). All of these groups are involved in various metabolic pathways, in particular galactose metabolism, glyoxylate and dicarboxylate metabolism, and starch and sucrose metabolism. These results suggested that energy metabolism is important at the metabolic level in dormancy release following GA4 treatment. We also found that more than 10-fold differences in abundance were observed for many metabolites, including sucrose, proline, linoleic acid, and linolenic acid, which might play important roles during the dormancy process. The current research extends our understanding of the mechanisms involved in budburst and dormancy release in response to GA4 and provides a theoretical basis for applying GA4 to release dormancy. PMID:26504583

  2. Metabolic changes upon flower bud break in Japanese apricot are enhanced by exogenous GA4

    PubMed Central

    Zhuang, Weibing; Gao, Zhihong; Wen, Luhua; Huo, Ximei; Cai, Binhua; Zhang, Zhen

    2015-01-01

    Gibberellin (GA4) has a significant effect on promoting dormancy release in flower buds of Japanese apricot (Prunus mume Sieb. et Zucc). The transcriptomic and proteomic changes that occur after GA4 treatment have been reported previously; however, the metabolic changes brought about by GA4 remain unknown. The present study was undertaken to assess changes in metabolites in response to GA4 treatment, as determined using gas chromatography–mass spectrometry and principal component analysis. Fifty-five metabolites that exhibited more than two-fold differences in abundance (P < 0.05) between samples collected over time after a given treatment or between samples exposed to different treatments were studied further. These metabolites were categorized into six main groups: amino acids and their isoforms (10), amino acid derivatives (7), sugars and polyols (14), organic acids (12), fatty acids (4), and others (8). All of these groups are involved in various metabolic pathways, in particular galactose metabolism, glyoxylate and dicarboxylate metabolism, and starch and sucrose metabolism. These results suggested that energy metabolism is important at the metabolic level in dormancy release following GA4 treatment. We also found that more than 10-fold differences in abundance were observed for many metabolites, including sucrose, proline, linoleic acid, and linolenic acid, which might play important roles during the dormancy process. The current research extends our understanding of the mechanisms involved in budburst and dormancy release in response to GA4 and provides a theoretical basis for applying GA4 to release dormancy. PMID:26504583

  3. Comparative proteomic and transcriptomic approaches to address the active role of GA4 in Japanese apricot flower bud dormancy release

    PubMed Central

    Zhuang, Weibing; Gao, Zhihong; Zhang, Zhen

    2013-01-01

    Hormones are closely associated with dormancy in deciduous fruit trees, and gibberellins (GAs) are known to be particularly important. In this study, we observed that GA4 treatment led to earlier bud break in Japanese apricot. To understand better the promoting effect of GA4 on the dormancy release of Japanese apricot flower buds, proteomic and transcriptomic approaches were used to analyse the mechanisms of dormancy release following GA4 treatment, based on two-dimensional gel electrophoresis (2-DE) and digital gene expression (DGE) profiling, respectively. More than 600 highly reproducible protein spots (P<0.05) were detected and, following GA4 treatment, 38 protein spots showed more than a 2-fold difference in expression, and 32 protein spots were confidently identified according to the databases. Compared with water treatment, many proteins that were associated with energy metabolism and oxidation–reduction showed significant changes after GA4 treatment, which might promote dormancy release. We observed that genes at the mRNA level associated with energy metabolism and oxidation–reduction also played an important role in this process. Analysis of the functions of the identified proteins and genes and the related metabolic pathways would provide a comprehensive proteomic and transcriptomic view of the coordination of dormancy release after GA4 treatment in Japanese apricot flower buds. PMID:24014872

  4. Latitudinal variation in sensitivity of flower bud formation to high temperature in Japanese Taraxacum officinale.

    PubMed

    Yoshie, Fumio

    2014-05-01

    Control of flowering time plays a key role in the successful range expansion of plants. Taraxacum officinale has expanded throughout Japan during the 110 years after it was introduced into a cool temperate region. The present study tested a hypothesis that there is a genetic difference in the bud formation time in relation to temperature along latitudinal gradient of T. officinale populations. In Experiment 1, plants from three populations at different latitudes (26, 36, and 43°N) were grown at three temperatures. Time to flower bud appearance did not significantly differ among the three populations when plants were grown at 14 °C, whereas it increased with increasing latitude when grown at 19 and 24 °C. Rosette diameter was not different among the populations, indicating that the variation in bud formation time reflected a difference in genetic control rather than size variation. The latitudinal variation in bud appearance time was confirmed by Experiment 2 in which plants from 17 population were used. In Experiment 3, the size of plants that exhibited late-flowering was studied to test a hypothesis that the variation in flowering time reflects dormancy of vegetative growth, but the late-flowering plants were found to continue growth, indicating that vegetative dormancy was not the cause of the variation. The results clearly indicate that the degree of suppression of flower bud formation at high temperature decreases with latitude from north to south, which is under genetic control. PMID:24585133

  5. Flower color changes in three Japanese hibiscus species: further quantitative variation of anthocyanin and flavonols.

    PubMed

    Shimokawa, Satoshi; Iwashina, Tsukasa; Murakami, Noriaki

    2015-03-01

    One anthocyanin and four flavonols were detected from the petals of Hibiscus hamabo, H. tiliaceus and H. glaber. They were identified as cyanidin 3-0- sambubioside, gossypetin 3-O-glucuronide-8-O-glucoside, quercetin 7-O-rutinoside, gossypetin 3-O-glucoside and gossypetin 8-O-glucuronide by UV spectra, LC-MS, acid hydrolysis and HPLC. The flavonoid composition was essentially the same among the petals ofH. hamabo, H. tiliaceus and H. glaber, and there was little quantitative variation, except for cyanidin 3-O-sambubioside, the content of which in the petals ofH. tiliaceus and H. glaber was much higher than in that of H. hamabo. Flower colors of H. tiliaceus and H. glaber change from yellow to red, and that of H. hamabo changes from yellow to orange. These changes were caused by contents of anthocyanin and flavonols, which increased after flowering of H. hamabo, H. tiliaceus and H. glaber. PMID:25924527

  6. A Stowaway transposon disrupts the InWDR1 gene controlling flower and seed coloration in a medicinal cultivar of the Japanese morning glory.

    PubMed

    Hoshino, Atsushi; Yoneda, Yoshiaki; Kuboyama, Tsutomu

    2016-07-20

    Floricultural cultivars of the Japanese morning glory (Ipomoea nil) carry transposons of the Tpn1 family as active spontaneous mutagens. Half of the characterized mutations related to floricultural traits were caused by insertion of Tpn1 family elements. In addition, mutations comprising insertions of several bp, presumed to be footprints generated by transposon excisions, were also found. Among these, ca-1 and ca-2 are 7-bp insertions at the same position in the InWDR1 gene, which encodes a multifunctional transcription regulator. InWDR1 enhances anthocyanin pigmentation in blue flowers and red stems, and promotes dark brown seed pigmentation as well as seed-trichome formation. The recessive ca mutants show white flowers and whitish seeds. We characterized here a white flower and whitish seed line that is used as a medicinal herb. The mutant line carries a novel ca allele named ca-3, which is the InWDR1 gene carrying an insertion of a Stowaway-like transposon, InSto1. The ca-3 allele is the first example of a mutation induced by transposons other than those in the Tpn1 family in I. nil. Because InSto1 and the 7-bp putative footprints are inserted at identical positions in InWDR1, ca-3 is likely to be the ancestor of ca-1 and ca-2. According to Japanese historical records on whitish seeds of I. nil, putative ca mutants appeared at the end of the 17th century, at the latest. This is around one hundred years before the appearance of many floricultural mutants. This suggests that ca-3 is one of the oldest mutations, and that its origin is different from that of most floricultural mutations in I. nil. PMID:27074980

  7. Cherry-Slush-Candling Apparatus

    NASA Technical Reports Server (NTRS)

    Stephens, James B.; Weiss, James R.; Hoover, Gordon

    1996-01-01

    Proposed infrared-scanning apparatus for use in bakeries making cherry pies detect cherry pits remaining in cherry slush after pitting process. Pits detected via their relative opacity to infrared radiation.

  8. Using daily temperature to predict phenology trends in spring flowers

    NASA Astrophysics Data System (ADS)

    Kim, Jin-Hee; Kim, Soo-Ock; Kim, Dae-Jun; Moon, Kyung Hwan; Yun, Jin I.

    2015-05-01

    The spring season in Korea features a dynamic landscape with a variety of flowers blooming sequentially one after another. This enables local governments to earn substantial sightseeing revenues by hosting festivals featuring spring flowers. Furthermore, beekeepers move from the southern tip of the Korean Peninsula all the way northward in a quest to secure spring flowers as nectar sources for a sustained period of time. However, areal differences in flowering dates of flower species are narrowing, which has economic consequences. Analysis of data on flowering dates of forsythia ( Forsythia koreana) and cherry blossom ( Prunus serrulata), two typical spring flower species, as observed for the past 60 years at six weather stations of the Korea Meteorological Administration (KMA) indicated that the difference between the flowering date of forsythia, the earliest blooming flower in spring, and cherry blossom, which flowers later than forsythia, was 14 days on average in the climatological normal year for the period 1951-1980, compared with 11 days for the period 1981-2010. In 2014, the gap narrowed further to 7 days, making it possible in some locations to see forsythias and cherry blossoms blooming at the same time. Synchronized flowering of these two flower species is due to acceleration of flowering due to an abnormally high spring temperature, and this was more pronounced in the later-blooming cherry blossom than forsythia. While cherry blossom flowering dates across the nation ranged from March 31 to April 19 (an areal difference of 20 days) for the 1951-1980 normal year, the difference ranged from March 29 to April 12 (an areal difference of 16 days) for the 1981-2010 normal year, and in 2014, the flowering dates spanned March 25 and March 30 (an areal difference of 6 days). In the case of forsythia, the gap was narrower than in cherry blossoms. Climate change in the Korean Peninsula, reflected by rapid temperature hikes in late spring in contrast to a slow

  9. Effect of extending the photoperiod with low-intensity red or far-red light on the timing of shoot elongation and flower-bud formation of 1-year-old Japanese pear (Pyrus pyrifolia).

    PubMed

    Ito, Akiko; Saito, Takanori; Nishijima, Takaaki; Moriguchi, Takaya

    2014-05-01

    To investigate the effects of light quality (wavelength) on shoot elongation and flower-bud formation in Japanese pear (Pyrus pyrifolia (Burm. f.) Nakai), we treated 1-year-old trees with the following: (i) 8 h sunlight + 16 h dark (SD); (ii) 8 h sunlight + 16 h red light (LD(SD + R)); or (iii) 8 h sunlight + 16 h far-red (FR) light (LD(SD + FR)) daily for 4 months from early April (before the spring flush) until early August in 2009 and 2010. In both years, shoot elongation stopped earlier in the LD(SD + FR) treatment than in the SD and LD(SD + R) treatments. After 4 months of treatments, 21% (2009) or 40% (2010) of LD(SD + FR)-treated trees formed flower buds in the shoot apices, whereas all the shoot apices from SD or LD(SD + R)-treated plants remained vegetative. With an additional experiment conducted in 2012, we confirmed that FR light at 730 nm was the most efficacious wavelength to induce flower-bud formation. Reverse transcription-quantitative polymerase chain reaction revealed that the expression of two floral meristem identity gene orthologues, LEAFY (PpLFY2a) and APETALA1 (PpMADS2-1a), were up-regulated in the shoot apex of LD(SD + FR). In contrast, the expression of a flowering repressor gene, TERMINAL FLOWER 1 (PpTFL1-1a, PpTFL1-2a), was down-regulated. In addition, expression of an orthologue of the flower-promoting gene FLOWERING LOCUS T (PpFT1a) was positively correlated with flower-bud formation, although the expression of another orthologue, PpFT2a, was negatively correlated with shoot growth. Biologically active cytokinin and gibberellic acid concentrations in shoot apices were reduced with LD(SD + FR) treatment. Taken together, our results indicate that pear plants are able to regulate flowering in response to the R : FR ratio. Furthermore, LD(SD + FR) treatment terminated shoot elongation and subsequent flower-bud formation in the shoot apex at an earlier time, possibly by influencing the expression of flowering-related genes and modifying

  10. Flowers, Beautiful Flowers

    ERIC Educational Resources Information Center

    School Arts: The Art Education Magazine for Teachers, 2005

    2005-01-01

    In the lesson described, the middle school students had been studying the artist Georgia O'Keeffe and the history of her work. Students enhanced their flower portraits by adding a matching border and connecting the lesson to other subject areas. Students dissected a flower and drew a small diagram of the flower and labeled the parts. This is an…

  11. Involvement of EARLY BUD-BREAK, an AP2/ERF Transcription Factor Gene, in Bud Break in Japanese Pear (Pyrus pyrifolia Nakai) Lateral Flower Buds: Expression, Histone Modifications and Possible Target Genes.

    PubMed

    Anh Tuan, Pham; Bai, Songling; Saito, Takanori; Imai, Tsuyoshi; Ito, Akiko; Moriguchi, Takaya

    2016-05-01

    In the Japanese pear (Pyrus pyrifolia Nakai) 'Kosui', three developmental stages of lateral flower buds have been proposed to occur during ecodormancy to the flowering phase, i.e. rapid enlargement, sprouting and flowering. Here, we report an APETALA2/ethylene-responsive factor (AP2/ERF) transcription factor gene, named pear EARLY BUD-BREAK (PpEBB), which was highly expressed during the rapid enlargement stage occurring prior to the onset of bud break in flower buds. Gene expression analysis revealed that PpEBB expression was dramatically increased during the rapid enlargement stage in three successive growing seasons. PpEBB transcript levels peaked 1 week prior to onset of bud break in 'Kosui' potted plants treated with hydrogen cyanamide or water under forcing conditions. Chromatin immunoprecipitation-quantitative PCR showed that higher levels of active histone modifications (trimethylation of the histone H3 tail at Lys4) in the 5'-upstream and start codon regions of the PpEBB gene were associated with the induced expression level of PpEBB during the rapid enlargement stage. In addition, we provide evidence that PpEBB may interact with and regulate pear four D-type cyclin (PpCYCD3) genes during bud break in 'Kosui' lateral flower buds. PpEBB significantly increased the promoter activities of four PpCYCD3 genes in a dual-luciferase assay using tobacco leaves. Taken together, our findings uncovered aspects of the bud break regulatory mechanism in the Japanese pear and provided further evidence that the EBB family plays an important role in bud break in perennial plants. PMID:26940832

  12. Inheritance of pink flower color in Styrax japonicus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Most Styrax japonicus (Japanese snowbell) cultivars produce white flowers, but a few pink-flowered forms have been reported. ‘Pink Chimes’ is the most widely grown pink-flowered form and the only S. japonicus cultivar with deep pink flowers that hold their color even under hot growing conditions. ...

  13. 7 CFR 930.3 - Cherries.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Cherries. 930.3 Section 930.3 Agriculture Regulations... Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES OF... Definitions § 930.3 Cherries. Cherries means all tart/sour cherry varieties grown in the production...

  14. 7 CFR 930.3 - Cherries.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Cherries. 930.3 Section 930.3 Agriculture Regulations... Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES OF... Definitions § 930.3 Cherries. Cherries means all tart/sour cherry varieties grown in the production...

  15. 7 CFR 930.3 - Cherries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Cherries. 930.3 Section 930.3 Agriculture Regulations... Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES OF... Definitions § 930.3 Cherries. Cherries means all tart/sour cherry varieties grown in the production...

  16. 7 CFR 930.3 - Cherries.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Cherries. 930.3 Section 930.3 Agriculture Regulations... ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES OF... Definitions § 930.3 Cherries. Cherries means all tart/sour cherry varieties grown in the production...

  17. 7 CFR 930.3 - Cherries.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Cherries. 930.3 Section 930.3 Agriculture Regulations... ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES OF... Definitions § 930.3 Cherries. Cherries means all tart/sour cherry varieties grown in the production...

  18. Flower Parts.

    ERIC Educational Resources Information Center

    Steinheimer, Margaret

    1997-01-01

    Presents ideas for spring flower displays. Has students constructing their own interactive flower displays as extra-credit assignments to reinforce vocabulary and scientific concepts, and modeling flowers with household items. (JRH)

  19. Spring frost vulnerability of sweet cherries under controlled conditions

    NASA Astrophysics Data System (ADS)

    Matzneller, Philipp; Götz, Klaus-P.; Chmielewski, Frank-M.

    2016-01-01

    Spring frost is a significant production hazard in nearly all temperate fruit-growing regions. Sweet cherries are among the first fruit varieties starting their development in spring and therefore highly susceptible to late frost. Temperatures at which injuries are likely to occur are widely published, but their origin and determination methods are not well documented. In this study, a standardized method was used to investigate critical frost temperatures for the sweet cherry cultivar `Summit' under controlled conditions. Twigs were sampled at four development stages ("side green," "green tip," "open cluster," "full bloom") and subjected to three frost temperatures (-2.5, -5.0, -10.0 °C). The main advantage of this method, compared to other approaches, was that the exposition period and the time interval required to reach the target temperature were always constant (2 h). Furthermore, then, the twigs were placed in a climate chamber until full bloom, before the examination of the flowers and not further developed buds started. For the first two sampling stages (side green, green tip), the number of buds found in open cluster, "first white," and full bloom at the evaluation date decreased with the strength of the frost treatment. The flower organs showed different levels of cold hardiness and became more vulnerable in more advanced development stages. In this paper, we developed four empirical functions which allow calculating possible frost damages on sweet cherry buds or flowers at the investigated development stages. These equations can help farmers to estimate possible frost damages on cherry buds due to frost events. However, it is necessary to validate the critical temperatures obtained in laboratory with some field observations.

  20. Bird Cherry-Oat Aphid

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bird cherry-oat aphid feeds on barley, oats, rye, triticale, and wheat by sucking plant juices. Its feeding may stunt plants and lead to yield loss, but it does not cause symptoms of yellowing and leaf curling. Bird cherry-oat aphid is also a vector of barley yellow dwarf virus. Biological, cultu...

  1. 7 CFR 923.5 - Cherries.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Cherries. 923.5 Section 923.5 Agriculture Regulations... Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE SWEET CHERRIES GROWN IN DESIGNATED COUNTIES IN WASHINGTON Order Regulating Handling Definitions § 923.5 Cherries. Cherries means all varieties...

  2. 7 CFR 923.5 - Cherries.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Cherries. 923.5 Section 923.5 Agriculture Regulations... ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE SWEET CHERRIES GROWN IN DESIGNATED COUNTIES IN WASHINGTON Order Regulating Handling Definitions § 923.5 Cherries. Cherries means all varieties...

  3. 7 CFR 923.5 - Cherries.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Cherries. 923.5 Section 923.5 Agriculture Regulations... Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE SWEET CHERRIES GROWN IN DESIGNATED COUNTIES IN WASHINGTON Order Regulating Handling Definitions § 923.5 Cherries. Cherries means all varieties...

  4. 7 CFR 923.5 - Cherries.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Cherries. 923.5 Section 923.5 Agriculture Regulations... ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE SWEET CHERRIES GROWN IN DESIGNATED COUNTIES IN WASHINGTON Order Regulating Handling Definitions § 923.5 Cherries. Cherries means all varieties...

  5. 7 CFR 923.5 - Cherries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Cherries. 923.5 Section 923.5 Agriculture Regulations... Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE SWEET CHERRIES GROWN IN DESIGNATED COUNTIES IN WASHINGTON Order Regulating Handling Definitions § 923.5 Cherries. Cherries means all varieties...

  6. Blob Flowers.

    ERIC Educational Resources Information Center

    Canfield, Elaine

    2003-01-01

    Describes an art project called blob flowers in which fifth-grade students created pictures of flowers using watercolor and markers. Explains that the lesson incorporates ideas from art and science. Discusses in detail how the students created their flowers. (CMK)

  7. Maraschino Cherry: A Laboratory-Lecture Unit

    ERIC Educational Resources Information Center

    Wrolstad, Ronald E.

    2009-01-01

    Oregon State Univ. has offered FST 102 "Maraschino Cherry" as a 1-credit orientation course since 1994. The maraschino cherry serves as a vehicle from which faculty give their disciplinary perspective, for example, the chemistry of the maraschino cherry, processing unit operations, microbiology and food safety, food law, sensory analysis, product…

  8. Flower Development

    PubMed Central

    Alvarez-Buylla, Elena R.; Benítez, Mariana; Corvera-Poiré, Adriana; Chaos Cador, Álvaro; de Folter, Stefan; Gamboa de Buen, Alicia; Garay-Arroyo, Adriana; García-Ponce, Berenice; Jaimes-Miranda, Fabiola; Pérez-Ruiz, Rigoberto V.; Piñeyro-Nelson, Alma; Sánchez-Corrales, Yara E.

    2010-01-01

    Flowers are the most complex structures of plants. Studies of Arabidopsis thaliana, which has typical eudicot flowers, have been fundamental in advancing the structural and molecular understanding of flower development. The main processes and stages of Arabidopsis flower development are summarized to provide a framework in which to interpret the detailed molecular genetic studies of genes assigned functions during flower development and is extended to recent genomics studies uncovering the key regulatory modules involved. Computational models have been used to study the concerted action and dynamics of the gene regulatory module that underlies patterning of the Arabidopsis inflorescence meristem and specification of the primordial cell types during early stages of flower development. This includes the gene combinations that specify sepal, petal, stamen and carpel identity, and genes that interact with them. As a dynamic gene regulatory network this module has been shown to converge to stable multigenic profiles that depend upon the overall network topology and are thus robust, which can explain the canalization of flower organ determination and the overall conservation of the basic flower plan among eudicots. Comparative and evolutionary approaches derived from Arabidopsis studies pave the way to studying the molecular basis of diverse floral morphologies. PMID:22303253

  9. Validation of the hybrid flowering cherry Prunus xincam Ingram (Rosaceae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This contribution provides scientific validation for a widely-used scientific name of a nothospecies in Prunus sect. Pseudocerasus. This name has been used informally for many years, but without the publication of a full description in a recognized journal and the designation of a type specimen the ...

  10. Ireland's Cherry Orchard National School

    ERIC Educational Resources Information Center

    O Cuiv, Shan

    2007-01-01

    This recently completed primary school illustrates how architecture can contribute to creating a safe and warm environment in a difficult area and can meet the particular needs of the student community. In its first year in operation, Cherry Orchard National School is proving to be a successful project. Presented here are the architectural…

  11. Bird Cherry-Oat Aphid

    Technology Transfer Automated Retrieval System (TEKTRAN)

    • The bird cherry-oat aphid (Rhopalosiphum padi) is an important vector of barley yellow dwarf viruses that affect wheat and other small-grain crops, but the aphid may also cause direct feeding damage to wheat. • Various plant-resistance modalities and natural enemies are not equally applicable in s...

  12. Chopping Down the Cherry Tree.

    ERIC Educational Resources Information Center

    Griswold, Jerry

    1995-01-01

    Attempts once again to put to rest the infamous "I cannot tell a lie" episode involving George Washington and a downed cherry tree. Appends an editor's note that states that William Bennett's "The Children's Book of Virtues" which perpetuates this infamous piece of "fakelore." (RS)

  13. Japanese; Japanese Songs.

    ERIC Educational Resources Information Center

    Defense Language Inst., Washington, DC.

    This supplementary textbook for students of Japanese presents a collection of 43 songs--folk songs, nursery songs, lullabies, love songs, wedding songs, graduation songs, the national anthem, drinking songs, school songs, and Christmas carols. With the exception of the carols, the musical scores are presented with their Japanese lyrics. The…

  14. Flowers & Weeds.

    ERIC Educational Resources Information Center

    Flannery, Maura C.

    1996-01-01

    Describes the topics and teaching strategies employed in an Issues in Biology course. Discusses flowers, plant breeding, potatoes and tomatoes, the chocolate tree, weeds, Arabidopis, gene transfers, and plant genes/human genes. Contains 22 references. (JRH)

  15. 77 FR 12103 - Culturally Significant Objects Imported for Exhibition Determinations: “Colorful Realm: Japanese...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ...-Flower Paintings by It Jakuch (1716-1800)'' SUMMARY: Notice is hereby given of the following... exhibition ``Colorful Realm: Japanese Bird-and-Flower Paintings by It Jakuch (1716-1800),'' imported...

  16. Open cherry picker simulation results

    NASA Technical Reports Server (NTRS)

    Nathan, C. A.

    1982-01-01

    The simulation program associated with a key piece of support equipment to be used to service satellites directly from the Shuttle is assessed. The Open Cherry Picker (OCP) is a manned platform mounted at the end of the remote manipulator system (RMS) and is used to enhance extra vehicular activities (EVA). The results of simulations performed on the Grumman Large Amplitude Space Simulator (LASS) and at the JSC Water Immersion Facility are summarized.

  17. Sexual regeneration traits linked to black cherry ( Prunus serotina Ehrh.) invasiveness

    NASA Astrophysics Data System (ADS)

    Pairon, Marie; Chabrerie, Olivier; Casado, Carolina Mainer; Jacquemart, Anne-Laure

    2006-09-01

    In order to better understand the invasive capacity of black cherry ( Prunus serotina Ehrh.), the regeneration dynamics of the species was studied during two consecutive years in a Belgian Pine plantation. Flower and fruit production, seed rain, dispersal and viability as well as the survival of seedlings of different ages were assessed. Despite the low fruit/flower ratio, fruit production was high (up to 8940 fruits per tree) as trees produced huge quantities of flowers. Both flower and fruit productions were highly variable between years and among individuals. The production variability between individuals was not correlated with plant size variables. Fruits were ripe in early September and a majority fell in the vicinity of the parent tree. A wide range of bird species dispersed 18% of the fruits at the end of October. Sixty-two percent of the fruits were viable and mean densities of 611 fruits m -2 were recorded on the forest floor. High mortality among young seedlings was observed and 95.3% of the fruits failed to give 4-year-old saplings. Nevertheless, the few saplings older than 4 years (1.32 m -2) presented a high survival rate (86%). All these regeneration traits are discussed in order to determine the main factors explaining the black cherry invasive success in Europe.

  18. Speed, Acceleration, Chameleons and Cherry Pit Projectiles

    ERIC Educational Resources Information Center

    Planinsic, Gorazd; Likar, Andrej

    2012-01-01

    The paper describes the mechanics of cherry pit projectiles and ends with showing the similarity between cherry pit launching and chameleon tongue projecting mechanisms. The whole story is written as an investigation, following steps that resemble those typically taken by scientists and can therefore serve as an illustration of scientific…

  19. Speed, acceleration, chameleons and cherry pit projectiles

    NASA Astrophysics Data System (ADS)

    Planinsic, Gorazd; Likar, Andrej

    2012-01-01

    The paper describes the mechanics of cherry pit projectiles and ends with showing the similarity between cherry pit launching and chameleon tongue projecting mechanisms. The whole story is written as an investigation, following steps that resemble those typically taken by scientists and can therefore serve as an illustration of scientific reasoning and how scientific knowledge is built.

  20. 21 CFR 145.126 - Artificially sweetened canned cherries.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Artificially sweetened canned cherries. 145.126... § 145.126 Artificially sweetened canned cherries. (a) Artificially sweetened canned cherries is the food which conforms to the definition and standard of identity prescribed for canned cherries by §...

  1. 7 CFR 930.15 - Restricted percentage cherries.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Restricted percentage cherries. 930.15 Section 930.15... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES... Handling Definitions § 930.15 Restricted percentage cherries. Restricted percentage cherries means...

  2. 7 CFR 930.8 - Free market tonnage percentage cherries.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Free market tonnage percentage cherries. 930.8 Section... Order Regulating Handling Definitions § 930.8 Free market tonnage percentage cherries. Free market tonnage percentage cherries means that proportion of cherries handled in a crop year which are free to...

  3. 7 CFR 930.8 - Free market tonnage percentage cherries.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Free market tonnage percentage cherries. 930.8 Section... Order Regulating Handling Definitions § 930.8 Free market tonnage percentage cherries. Free market tonnage percentage cherries means that proportion of cherries handled in a crop year which are free to...

  4. 7 CFR 930.8 - Free market tonnage percentage cherries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Free market tonnage percentage cherries. 930.8 Section... Order Regulating Handling Definitions § 930.8 Free market tonnage percentage cherries. Free market tonnage percentage cherries means that proportion of cherries handled in a crop year which are free to...

  5. 7 CFR 930.8 - Free market tonnage percentage cherries.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Free market tonnage percentage cherries. 930.8 Section... Order Regulating Handling Definitions § 930.8 Free market tonnage percentage cherries. Free market tonnage percentage cherries means that proportion of cherries handled in a crop year which are free to...

  6. 7 CFR 930.15 - Restricted percentage cherries.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Restricted percentage cherries. 930.15 Section 930.15... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES... Handling Definitions § 930.15 Restricted percentage cherries. Restricted percentage cherries means...

  7. 7 CFR 930.15 - Restricted percentage cherries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Restricted percentage cherries. 930.15 Section 930.15... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES... Handling Definitions § 930.15 Restricted percentage cherries. Restricted percentage cherries means...

  8. 7 CFR 930.15 - Restricted percentage cherries.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Restricted percentage cherries. 930.15 Section 930.15... AGREEMENTS AND ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES... Handling Definitions § 930.15 Restricted percentage cherries. Restricted percentage cherries means...

  9. 21 CFR 145.126 - Artificially sweetened canned cherries.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Artificially sweetened canned cherries. 145.126... § 145.126 Artificially sweetened canned cherries. (a) Artificially sweetened canned cherries is the food which conforms to the definition and standard of identity prescribed for canned cherries by §...

  10. 7 CFR 930.15 - Restricted percentage cherries.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Restricted percentage cherries. 930.15 Section 930.15... AGREEMENTS AND ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES... Handling Definitions § 930.15 Restricted percentage cherries. Restricted percentage cherries means...

  11. Monitoring Drosophila suzukii Matsumura in Oregon, USA sweet cherry orchards.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Drosophila suzukii rapidly became a significant cherry pest in the western United States after it was observed damaging cherries in 2009 in California. It has caused significant damage to ripening cherries in all major USA cherry production districts leading to increased management costs and reduced...

  12. Cherry Irradiation Studies. 1984 annual report

    SciTech Connect

    Eakin, D.E.; Hungate, F.P.; Tingey, G.L.; Olsen, K.L.; Fountain, J.B.; Burditt, A.K. Jr.; Moffit, H.R.; Johnson, D.A.; Lunden, J.D.

    1985-04-01

    Fresh cherries, cherry fruit fly larvae, and codling moth larvae were irradiated using the PNL cobalt-60 facility to determine the efficacy of irradiation treatment for insect disinfestation and potential shelf life extension. Irradiation is an effective disinfestation treatment with no significant degradation of fruit at doses well above those required for quarantine treatment. Sufficient codling moth control was achieved at projected doses of less than 25 krad; cherry fruit fly control, at projected doses of less than 15 krad. Dose levels up to 60 krad did not adversely affect cherry quality factors tested. Irradiation above 60 krad reduced the firmness of cherries but had no significant impact on other quality factors tested. Irradiation of cherries below 80 krad did not result in any significant differences in sensory evaluations (appearance, flavor, and firmness) in tests conducted at OSU. Irradiation up to 200 krad at a temperature of about 25/sup 0/C (77/sup 0/F) did not measurably extend shelf life. Irradiation at 500 krad at 25/sup 0/C (77/sup 0/F) increased mold and rotting of cherries tested. There is no apparent advantage of irradiation over low-temperature fumigation.

  13. Flowers in Their Variety.

    ERIC Educational Resources Information Center

    Flannery, Maura C.

    2002-01-01

    Describes the diversity of flowers with regard to the flower paintings of Pierre-Joseph Redoute, books about flowers, and research in genetic studies. Discusses gardening flowers and flowering strategies and criticizes the fact that biology education has moved steadily away from plants. (KHR)

  14. Things Japanese.

    ERIC Educational Resources Information Center

    Shigeta, Jessie M.

    Presented in this booklet are brief descriptions of items and activities that are symbolic of Japanese culture. Some of the items and activities described include Japanese musical instruments and records, toys and crafts, traditional clothing and accessories, and food utensils. Several recipes for Japanese dishes are provided. Lists of pertinent…

  15. Elevation of grove looking northeast toward Washington Monument 1910 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Elevation of grove looking northeast toward Washington Monument - 1910 Japanese Flowering Cherry Trees , East Potomac Golf Course, East Potomac Park, Hains Point vicinity, Washington, District of Columbia, DC

  16. Japanese language and Japanese science

    NASA Astrophysics Data System (ADS)

    Tanikawa, Kiyotaka

    2003-08-01

    Japanese mathematical scientists including astronomers, physicists, and mathematicians obtain ideas in Japanese, discuss their problems in Japanese, and arrive at conclusions in Japanese, and yet they write their results in foreign languages such as English. This uncomfortable situation has continued for nearly one hundred years and has had serious effects on Japanese science. In this short report, the author discusses and analyses these effects. In order to put Japanese science on a sound basis, the author proposes to increase the number of articles, reviews and textbooks in Japanese, first by translation and second by the voluntary efforts of scientists themselves. As centers devoted to this activity, the author proposes to construct "Airborne Libraries" which are maintained and accumulate in an electronic form the scientific documents written in Japanese.

  17. Lynne Cherry's World: A Celebration of Nature.

    ERIC Educational Resources Information Center

    Winarski, Diana L.

    1995-01-01

    Profiles Lynne Cherry, author-illustrator whose books convey her interest in environmentalism and preserving natural resources. Describes her career, process of illustrating several recent books, and founding of the Center for Children's Environmental Literature in 1992. (TM)

  18. Invariant Measures for Cherry Flows

    NASA Astrophysics Data System (ADS)

    Saghin, Radu; Vargas, Edson

    2013-01-01

    We investigate the invariant probability measures for Cherry flows, i.e. flows on the two-torus which have a saddle, a source, and no other fixed points, closed orbits or homoclinic orbits. In the case when the saddle is dissipative or conservative we show that the only invariant probability measures are the Dirac measures at the two fixed points, and the Dirac measure at the saddle is the physical measure. In the other case we prove that there exists also an invariant probability measure supported on the quasi-minimal set, we discuss some situations when this other invariant measure is the physical measure, and conjecture that this is always the case. The main techniques used are the study of the integrability of the return time with respect to the invariant measure of the return map to a closed transversal to the flow, and the study of the close returns near the saddle.

  19. 75 FR 31663 - Sweet Cherries Grown in Designated Counties in Washington; Change in the Handling Regulation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-04

    ...This rule revises the handling regulation for cherries under the Washington cherry marketing order. The marketing order regulates the handling of sweet cherries grown in designated counties in Washington and is administered locally by the Washington Cherry Marketing Committee (Committee). This rule adds quality and pack requirements for Rainier cherries and other lightly-colored sweet cherry......

  20. Occurrence of Two Little Cherry Viruses in Sweet Cherry in Washington State

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Little cherry disease is one of the most damaging virus diseases of sweet cherries worldwide but remains poorly understood in both its distribution in the USA and the number of varieties of the disease that may occur. Diagnostic methods based on PCR for the LChV-1 strain were designed from the rep...

  1. 19. View of second floor of the Cherry Hill lettuce ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. View of second floor of the Cherry Hill lettuce shed looking at door to stairwell - Richmond Hill Plantation, Cherry Hill Lettuce Shed, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  2. 18. View of the second floor of the Cherry Hill ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. View of the second floor of the Cherry Hill lettuce shed looking at door - Richmond Hill Plantation, Cherry Hill Lettuce Shed, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  3. 15. Interior view of Cherry Hill lettuce shed showing posts ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Interior view of Cherry Hill lettuce shed showing posts looking towards the chute building - Richmond Hill Plantation, Cherry Hill Lettuce Shed, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  4. 20. View of second floor to the Cherry Hill lettuce ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. View of second floor to the Cherry Hill lettuce shed looking at floor area - Richmond Hill Plantation, Cherry Hill Lettuce Shed, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  5. 14. Interior view of Cherry Hill lettuce shed looking towards ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Interior view of Cherry Hill lettuce shed looking towards chute building - Richmond Hill Plantation, Cherry Hill Lettuce Shed, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  6. 9. View of Cherry Hill lettuce shed looking north, with ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. View of Cherry Hill lettuce shed looking north, with chute building on the left - Richmond Hill Plantation, Cherry Hill Lettuce Shed, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  7. 3. View of Cherry Hill lettuce shed looking southeast; parking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. View of Cherry Hill lettuce shed looking southeast; parking lot in foreground - Richmond Hill Plantation, Cherry Hill Lettuce Shed, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  8. 12. Partial view of Cherry Hill lettuce shed looking northwest ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Partial view of Cherry Hill lettuce shed looking northwest showing office - Richmond Hill Plantation, Cherry Hill Lettuce Shed, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  9. 2. Oblique view of Cherry Hill lettuce shed looking south; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Oblique view of Cherry Hill lettuce shed looking south; chute building is in background - Richmond Hill Plantation, Cherry Hill Lettuce Shed, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  10. 6. Oblique view of Cherry Hill lettuce shed looking northeast, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Oblique view of Cherry Hill lettuce shed looking northeast, with chute building to the right - Richmond Hill Plantation, Cherry Hill Lettuce Shed, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  11. 16. Interior view of Cherry Hill lettuce shed looking up ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. Interior view of Cherry Hill lettuce shed looking up at the trusses of the second floor - Richmond Hill Plantation, Cherry Hill Lettuce Shed, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  12. 21. View of second floor of the Cherry Hill lettuce ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. View of second floor of the Cherry Hill lettuce shed looking towards window - Richmond Hill Plantation, Cherry Hill Lettuce Shed, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  13. 22. View of second floor of the Cherry Hill lettuce ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. View of second floor of the Cherry Hill lettuce shed looking at double doors - Richmond Hill Plantation, Cherry Hill Lettuce Shed, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  14. 21 CFR 152.126 - Frozen cherry pie.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Frozen cherry pie. 152.126 Section 152.126 Food... HUMAN CONSUMPTION FRUIT PIES Requirements for Specific Standardized Fruit Pies § 152.126 Frozen cherry pie. (a) Identity. (1) Frozen cherry pie (excluding baked and then frozen) is the food prepared...

  15. 21 CFR 152.126 - Frozen cherry pie.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Frozen cherry pie. 152.126 Section 152.126 Food... HUMAN CONSUMPTION FRUIT PIES Requirements for Specific Standardized Fruit Pies § 152.126 Frozen cherry pie. (a) Identity. (1) Frozen cherry pie (excluding baked and then frozen) is the food prepared...

  16. 21 CFR 152.126 - Frozen cherry pie.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen cherry pie. 152.126 Section 152.126 Food... HUMAN CONSUMPTION FRUIT PIES Requirements for Specific Standardized Fruit Pies § 152.126 Frozen cherry pie. (a) Identity. (1) Frozen cherry pie (excluding baked and then frozen) is the food prepared...

  17. 21 CFR 152.126 - Frozen cherry pie.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Frozen cherry pie. 152.126 Section 152.126 Food... HUMAN CONSUMPTION FRUIT PIES Requirements for Specific Standardized Fruit Pies § 152.126 Frozen cherry pie. (a) Identity. (1) Frozen cherry pie (excluding baked and then frozen) is the food prepared...

  18. 21 CFR 152.126 - Frozen cherry pie.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Frozen cherry pie. 152.126 Section 152.126 Food... HUMAN CONSUMPTION FRUIT PIES Requirements for Specific Standardized Fruit Pies § 152.126 Frozen cherry pie. (a) Identity. (1) Frozen cherry pie (excluding baked and then frozen) is the food prepared...

  19. 7 CFR 930.8 - Free market tonnage percentage cherries.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Free market tonnage percentage cherries. 930.8 Section... CHERRIES GROWN IN THE STATES OF MICHIGAN, NEW YORK, PENNSYLVANIA, OREGON, UTAH, WASHINGTON, AND WISCONSIN Order Regulating Handling Definitions § 930.8 Free market tonnage percentage cherries. Free...

  20. Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Little cherry virus 1 (LChV-1), associated with little cherry disease (LCD), has a significant impact on fruit quality of infected sweet cherry trees. We report the full genome sequence of an isolate of LChV-1 from China, detected by small RNA deep sequencing and amplified by overlapping RT-PCR. The...

  1. On-Line Sorting Maturity of Cherry Tomato Bymachine Vision

    NASA Astrophysics Data System (ADS)

    Zhang, Yinglong; Yin, Xiaoping; Xu, Tongyu; Zhao, Jiewen

    The cherry tomatoes online sorting according to their maturity is an important procedure after harvest. This research proposed an automated cherry tomato grading system base on machine vision. Three images of different angles are obtained from each cherry tomato, allowing the inspection of approximately 90% of the fruit surface. 9 features were extracted from the one cherry tomato images. In order to distinguish into three grades (immature, half ripe and ripe), Principal component analysis (PCA) and linear discrimination analysis (LDA) were used to analyze the features. The PCA results show that ripe cherry tomatoes are distinguished from immature and half ripe ones. 414 cherry tomatoes were tested by the online sorting system. The overall accuracy was up to 94.9%. Furthermore, the grading speed of the sorting line reaches 7 cherry tomatoes per second which meet the actual demand of many farms.

  2. Complete nucleotide sequence of cherry virus A (CVA) infecting sweet cherry in India.

    PubMed

    Noorani, M S; Awasthi, P; Singh, Rahul Mohan; Ram, Raja; Sharma, M P; Singh, S R; Ahmed, N; Hallan, V; Zaidi, A A

    2010-12-01

    Cherry virus A (CVA) is a graft-transmissible member of the genus Capillovirus that infects different stone fruits. Sweet cherry (Prunus avium L; family Rosaceae) is an important deciduous temperate fruit crop in the Western Himalayan region of India. In order to determine the health status of cherry plantations and the incidence of the virus in India, cherry orchards in the states of Jammu and Kashmir (J&K) and Himachal Pradesh (H.P.) were surveyed during the months of May and September 2009. The incidence of CVA was found to be 28 and 13% from J&K and H.P., respectively, by RT-PCR. In order to characterize the virus at the molecular level, the complete genome was amplified by RT-PCR using specific primers. The amplicon of about 7.4 kb was sequenced and was found to be 7,379 bp long, with sequence specificity to CVA. The genome organization was similar to that of isolates characterized earlier, coding for two ORFs, in which ORF 2 is nested in ORF1. The complete sequence was 81 and 84% similar to that of the type isolate at the nucleotide and amino acid level, respectively, with 5' and 3' UTRs of 54 and 299 nucleotides, respectively. This is the first report of the complete nucleotide sequence of cherry virus A infecting sweet cherry in India. PMID:20938696

  3. Detection of pits in fresh cherries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    There are a number of x-ray imaging techniques that could be implemented for the detection of pits in cherries, including linescan and real-time imaging using an image intensifier and CCD camera. However, x-ray imaging equipment is both expensive and bulky, and implementation on the processing line ...

  4. Lynne Cherry's "A River Ran Wild."

    ERIC Educational Resources Information Center

    Ledford, Carolyn; Brent, Rebecca

    1997-01-01

    Paraphrases the book "A River Ran Wild" by Lynne Cherry, contrasts how Native American and European settlers use a river, and discusses the pollution and cleanup of the river. Provides classroom discussion questions, and individual or group activities in language arts, art, role-playing, geography, and interviewing. Includes an annotated…

  5. Design a Hummingbird Flower.

    ERIC Educational Resources Information Center

    Bailey, Kim

    2002-01-01

    Presents an activity that engages students in designing and making an artificial flower adapted for pollination by hummingbirds. Students work in teams to design flowers that maximize the benefit from attracting hummingbirds. Examines characteristics of real flowers adapted to pollination by hummingbirds. (DLH)

  6. Development of Anastrepha fraterculus (Diptera: Tephritidae) Related to the Phenology of Blueberry, Blackberry, Strawberry Guava, and Surinam Cherry Fruits.

    PubMed

    Bisognin, M; Nava, D E; Diez-Rodríguez, G I; Valgas, R A; Garcia, M S; Krolow, A C R; Antunes, L E C

    2015-02-01

    Anastrepha fraterculus (Wiedemann, 1830) is the main pest of temperate climate orcharding. The study investigated the development of A. fraterculus related to phenological stage of blueberry, blackberry, strawberry guava, and Surinam cherry trees. The phenological stages I (green fruits), II (intermediate ripening stage of fruits), and III (fruits close to harvesting) were determined, and they are from 8th, 10th, and 11th week; 6th, 8th, and 9th week; 8th, 13th, and 16th week; and 5th, 6th, and 7th week after the first flowering of blueberry, blackberry, strawberry guava, and Surinam cherry trees, respectively. We collected fruits from orchards to determine the infestation index using the formula: number of pupa/fruit weight. To investigate the development of A. fraterculus, we determined the following biological parameters: egg-to-adult period, weight of pupae, oviposition period, fecundity, number of pupae, and number of infested fruits. The infestation index for the fruits collected in the field was greater in strawberry guava and Surinam cherry fruits. In the laboratory, the development of A. fraterculus occurred in stage III of blueberry. In blackberry, besides stage III, we also observed the development in stage II, however, at lower infestation. In strawberry guava, the development of A. fraterulus occurred in stages II and III, and the development in both stages was similar. For Surinam cherry, the development occurred in the three phenological stages with similar values for biological parameters. Overall, of the four hosts studied, the strawberry guava and Surinam cherry fruits allowed a better biological development of A. fraterculus, corroborating its preference for fruits native to Brazil. PMID:26470120

  7. The oxygen supply to thermogenic flowers.

    PubMed

    Seymour, Roger S; Ito, Kikukatsu; Umekawa, Yui; Matthews, Philip D G; Pirintsos, Stergios Arg

    2015-04-01

    Thermogenic flowers produce heat by intense respiration, and the rates of O2 consumption (Ṁo2) in some species can exceed those of all other tissues of plants and most animals. By exposing intact flowers to a range of O2 pressures (Po2) and measuring Ṁo2, we demonstrate that the highest respiration rates exceed the capacity of the O2 diffusive pathway and become diffusion limited in atmospheric air. The male florets on the inflorescence of Arum concinnatum have the highest known mass-specific Ṁo2 and can be severely diffusion limited. Intact spadices of Japanese skunk cabbage Symplocarpus renifolius are diffusion limited in air only when Ṁo2 is maximal, but not at lower levels. True flowers of the sacred lotus Nelumbo nucifera and the appendix of Arum concinnatum are never diffusion limited in air. Ṁo2 - Po2 curves are evaluated quantitatively with the 'Regulation Index', a new tool to measure dependence of Ṁo2 on ambient Po2 , as well as the conventional 'Critical Po2 '. The study also includes measurements of Po2 within thermogenic tissues with O2-sensitive fibre optics, and reveals that the diffusion pathway is complicated and that O2 can be provided not only from the surface of the tissues but also from the pith of the flower's peduncle. PMID:25256124

  8. Identification of chilling and heat requirements of cherry trees—a statistical approach

    NASA Astrophysics Data System (ADS)

    Luedeling, Eike; Kunz, Achim; Blanke, Michael M.

    2013-09-01

    Most trees from temperate climates require the accumulation of winter chill and subsequent heat during their dormant phase to resume growth and initiate flowering in the following spring. Global warming could reduce chill and hence hamper the cultivation of high-chill species such as cherries. Yet determining chilling and heat requirements requires large-scale controlled-forcing experiments, and estimates are thus often unavailable. Where long-term phenology datasets exist, partial least squares (PLS) regression can be used as an alternative, to determine climatic requirements statistically. Bloom dates of cherry cv. `Schneiders späte Knorpelkirsche' trees in Klein-Altendorf, Germany, from 24 growing seasons were correlated with 11-day running means of daily mean temperature. Based on the output of the PLS regression, five candidate chilling periods ranging in length from 17 to 102 days, and one forcing phase of 66 days were delineated. Among three common chill models used to quantify chill, the Dynamic Model showed the lowest variation in chill, indicating that it may be more accurate than the Utah and Chilling Hours Models. Based on the longest candidate chilling phase with the earliest starting date, cv. `Schneiders späte Knorpelkirsche' cherries at Bonn exhibited a chilling requirement of 68.6 ± 5.7 chill portions (or 1,375 ± 178 chilling hours or 1,410 ± 238 Utah chill units) and a heat requirement of 3,473 ± 1,236 growing degree hours. Closer investigation of the distinct chilling phases detected by PLS regression could contribute to our understanding of dormancy processes and thus help fruit and nut growers identify suitable tree cultivars for a future in which static climatic conditions can no longer be assumed. All procedures used in this study were bundled in an R package (`chillR') and are provided as Supplementary materials. The procedure was also applied to leaf emergence dates of walnut (cv. `Payne') at Davis, California.

  9. Japanese Characters in Written Japanese.

    ERIC Educational Resources Information Center

    Buck, James H.

    From the sixth to the eighth century A.D., Japan was the recipient of massive cultural infusions from China. This acceptance of the Chinese pattern included, and to a great extent was based on, the acceptance of the Chinese language. The Chinese writing system was applied to Japanese because there was no other model to follow and in spite of the…

  10. Bird cherry-oat aphid resistance in barley

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bird cherry-oat aphid, Rhopalosiphum padi L., is a serious pest of barley, Hordeum vulgare L., world-wide. It is the most efficient vector of barley yellow dwarf virus, the most important viral disease of small grains in the world. Not all bird cherry-oat aphids acquire the virus while feeding on ...

  11. 7 CFR 923.322 - Washington cherry handling regulation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... List of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and at... percent, by count, of such cherries in any lot must exhibit a pink-to-red surface blush and, for any given sample, not more than 20 percent of the cherries shall be absent a pink-to-red surface blush. (2)...

  12. 7 CFR 923.322 - Washington cherry handling regulation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... List of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and at... 7 Agriculture 8 2014-01-01 2014-01-01 false Washington cherry handling regulation. 923.322 Section... CHERRIES GROWN IN DESIGNATED COUNTIES IN WASHINGTON Order Regulating Handling Grade, Size, Container...

  13. 7 CFR 923.322 - Washington cherry handling regulation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... List of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and at... 7 Agriculture 8 2013-01-01 2013-01-01 false Washington cherry handling regulation. 923.322 Section... CHERRIES GROWN IN DESIGNATED COUNTIES IN WASHINGTON Order Regulating Handling Grade, Size, Container...

  14. 7 CFR 923.322 - Washington cherry handling regulation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... List of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and at... percent, by count, of such cherries in any lot must exhibit a pink-to-red surface blush and, for any given sample, not more than 20 percent of the cherries shall be absent a pink-to-red surface blush. (2)...

  15. First Report of Cherry virus A in Sweet Cherry Trees in China

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Plants in the genus Prunus of the family Rosaceae are important ornamental and fruit trees in China (1). In June 2007, sweet cherry (Prunus avium) trees with mottling and mosaic symptoms were observed in a private garden near Kunming, Yunnan Province. Twenty-four samples were then collected from swe...

  16. Oviposition in Sweet Cherry by Reproductively Mature Western Cherry Fruit Fly (Tephritidae:Diptera) Fed Spinosad and Neonicotinoid Insecticide Baits

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Western cherry fruit fly, Rhagoletis indifferens Curran, is a major pest of cherry, Prunus avium (L.) L., in the Pacific Northwest of the U.S. Spinosad bait is applied weekly to kill flies before they develop eggs, but its effects on oviposition by flies that are reproductively mature are unknown. ...

  17. Development and evaluation of a genome-wide 6K SNP array for diploid sweet cherry and tetraploid sour cherry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    High-throughput genome scans are important tools for genetic studies and breeding applications. Here, a 6K SNP array for use with the Illumina Infinium® system was developed for diploid sweet cherry (Prunus avium) and allotetraploid sour cherry (P. cerasus). This effort was led by RosBREED, a commun...

  18. 7 CFR 930.54 - Prohibition on the use or disposition of inventory reserve cherries.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... reserve cherries. 930.54 Section 930.54 Agriculture Regulations of the Department of Agriculture...), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES OF MICHIGAN, NEW YORK, PENNSYLVANIA, OREGON, UTAH... disposition of inventory reserve cherries. Cherries that are placed in inventory reserve pursuant to...

  19. 7 CFR 930.54 - Prohibition on the use or disposition of inventory reserve cherries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... reserve cherries. 930.54 Section 930.54 Agriculture Regulations of the Department of Agriculture...), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES OF MICHIGAN, NEW YORK, PENNSYLVANIA, OREGON, UTAH... disposition of inventory reserve cherries. Cherries that are placed in inventory reserve pursuant to...

  20. 7 CFR 930.54 - Prohibition on the use or disposition of inventory reserve cherries.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... reserve cherries. 930.54 Section 930.54 Agriculture Regulations of the Department of Agriculture...), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES OF MICHIGAN, NEW YORK, PENNSYLVANIA, OREGON, UTAH... disposition of inventory reserve cherries. Cherries that are placed in inventory reserve pursuant to...

  1. 7 CFR 930.54 - Prohibition on the use or disposition of inventory reserve cherries.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... reserve cherries. 930.54 Section 930.54 Agriculture Regulations of the Department of Agriculture...), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES OF MICHIGAN, NEW YORK, PENNSYLVANIA, OREGON, UTAH... disposition of inventory reserve cherries. Cherries that are placed in inventory reserve pursuant to...

  2. 7 CFR 930.54 - Prohibition on the use or disposition of inventory reserve cherries.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... reserve cherries. 930.54 Section 930.54 Agriculture Regulations of the Department of Agriculture...), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES OF MICHIGAN, NEW YORK, PENNSYLVANIA, OREGON, UTAH... disposition of inventory reserve cherries. Cherries that are placed in inventory reserve pursuant to...

  3. Prunus x sieboldii

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Siebold flowering cherry, Prunus x sieboldii, is an underutilized Japanese cherry ideal for today’s modern landscapes. First introduced from Japan nearly 150 years ago, Siebold cherry is now a rare taxon found primarily in botanic gardens and arboreta. The United States National Arboretum has one ...

  4. Say it with flowers

    PubMed Central

    Falik, Omer; Hoffmann, Ishay; Novoplansky, Ariel

    2014-01-01

    The timing of reproduction is a critical determinant of fitness, especially in organisms inhabiting seasonal environments. Increasing evidence suggests that inter-plant communication plays important roles in plant functioning. Here, we tested the hypothesis that flowering coordination can involve communication between neighboring plants. We show that soil leachates from Brassica rapa plants growing under long-day conditions accelerated flowering and decreased allocation to vegetative organs in target plants growing under non-inductive short-day conditions. The results suggest that besides endogenous signaling and external abiotic cues, flowering timing may involve inter-plant communication, mediated by root exudates. The study of flowering communication is expected to illuminate neglected aspects of plant reproductive interactions and to provide novel opportunities for controlling the timing of plant reproduction in agricultural settings. PMID:24598343

  5. Stars and Flowers, Flowers and Stars

    NASA Astrophysics Data System (ADS)

    Minti, Hari

    2012-12-01

    The author, a graduated from the Bucharest University (1964), actually living and working in Israel, concerns his book to variable stars and flowers, two domains of his interest. The analogies includes double stars, eclipsing double stars, eclipses, Big Bang. The book contains 34 chapters, each of which concerns various relations between astronomy and other sciences and pseudosciences such as Psychology, Religion, Geology, Computers and Astrology (to which the author is not an adherent). A special part of the book is dedicated to archeoastronomy and ethnoastronomy, as well as to history of astronomy. Between the main points of interest of these parts: ancient sanctuaries in Sarmizegetusa (Dacia), Stone Henge(UK) and other. The last chapter of the book is dedicated to flowers. The book is richly illustrated. It is designed for a wide circle of readers.

  6. Differences in body size and egg loads of Rhagoletis indifferens (Diptera: Tephritidae) from introduced and native cherries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The western cherry fruit fly, Rhagoletis indifferens Curran, infests introduced, domesticated sweet [Prunus avium (L.) L.] and tart cherries (Prunus cerasus L.) as well as native bitter cherry, Prunus emarginata (Douglas) Eaton. Bitter cherries are smaller than sweet and tart cherries and this coul...

  7. Handling Japanese without a Japanese Operating System.

    ERIC Educational Resources Information Center

    Hatasa, Kazumi; And Others

    1992-01-01

    The Macintosh HyperCard environment has become a popular platform for Japanese language courseware because of its flexibility and ease of programing. This project created Japanese bitmap font files for the JIS Levels 1 and 2, and writing XFCNs for font manipulation, Japanese kana input, and answer correction. (12 references) (Author/LB)

  8. Japanese encephalitis.

    PubMed

    Morita, K; Nabeshima, T; Buerano, C C

    2015-08-01

    Japanese encephalitis (JE) is an inflammation of the central nervous system in humans and animals, specifically horses and cattle. The disease, which can sometimes be fatal, is caused by the flavivirus Japanese encephalitis virus (JEV), of which there are five genotypes (genotypes 1, 2, 3, 4 and 5). The transmission cycle of the virus involves pigs and wild birds as virus amplifiers and mosquitoes as vectors for transferring the virus between amplifying hosts and to dead- end hosts, i.e. humans, horses and cattle. In horses and cattle the disease is usually asymptomatic, but when clinical signs do occur they include fever, decreased appetite, frothing at the mouth, rigidity of the legs and recumbency, and neurological signs, such as convulsive fits, circling, marked depression and disordered consciousness. In pigs, it can cause abortion and stillbirths. At present, the virus is detected in a wide area covering eastern and southern Asia, Indonesia, northern Australia, Papua New Guinea and Pakistan. JEV RNA has also been detected in Italy, first in dead birds in 1997 and 2000 and then in mosquitoes in 2010. Genotype shift, i.e. a change of genotype from genotype 3 to genotype 1, has occurred in some countries, namely Japan, South Korea, Chinese Taipei and Vietnam. Laboratory methods are available for confirming the causative agent of the disease. There are control measures to prevent or minimise infection and, among them, vaccination is one of the most important and one which should be adopted in endemic and epidemic areas. PMID:26601447

  9. Japanese encephalitis

    PubMed Central

    Yun, Sang-Im; Lee, Young-Min

    2014-01-01

    Japanese encephalitis (JE) is an infectious disease of the central nervous system caused by Japanese encephalitis virus (JEV), a zoonotic mosquito-borne flavivirus. JEV is prevalent in much of Asia and the Western Pacific, with over 4 billion people living at risk of infection. In the absence of antiviral intervention, vaccination is the only strategy to develop long-term sustainable protection against JEV infection. Over the past half-century, a mouse brain-derived inactivated vaccine has been used internationally for active immunization. To date, however, JEV is still a clinically important, emerging, and re-emerging human pathogen of global significance. In recent years, production of the mouse brain-derived vaccine has been discontinued, but 3 new cell culture-derived vaccines are available in various parts of the world. Here we review current aspects of JEV biology, summarize the 4 types of JEV vaccine, and discuss the potential of an infectious JEV cDNA technology for future vaccine development. PMID:24161909

  10. Development of the Potential for Cyanogenesis in Maturing Black Cherry (Prunus serotina Ehrh.) Fruits 1

    PubMed Central

    Swain, Elisabeth; Li, Chun Ping; Poulton, Jonathan E.

    1992-01-01

    Biochemical changes related to cyanogenesis (hydrogen cyanide production) were monitored during maturation of black cherry (Prunus serotina Ehrh.) fruits. At weekly intervals from flowering until maturity, fruits (or selected parts thereof) were analyzed for (a) fresh and dry weights, (b) prunasin and amygdalin levels, and (c) levels of the catabolic enzymes amygdalin hydrolase, prunasin hydrolase, and mandelonitrile lyase. During phase I (0-28 days after flowering [DAF]), immature fruits accumulated prunasin (mean: 3 micromoles/fruit) but were acyanogenic because they lacked the above enzymes. Concomitant with cotyledon development during mid-phase II, the seeds began accumulating both amygdalin (mean: 3 micromoles/seed) and the catabolic enzymes and were highly cyanogenic upon tissue disruption. Meanwhile, prunasin levels rapidly declined and were negligible by maturity. During phases II (29-65 DAF) and III (66-81 DAF), the pericarp also accumulated amygdalin, whereas its prunasin content declined toward maturity. Lacking the catabolic enzymes, the pericarp remained acyanogenic throughout all developmental stages. ImagesFigure 2Figure 4 PMID:16668810

  11. Molecular simultaneous detection of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus by real-time RT-PCR and high resolution melting analysis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In this study, real-time RT-PCR assays were combined with high resolution melting (HRM) analysis for the simultaneous detection of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV) infection in sweet cherry trees. Detection of CNRMV and CGRMV was performed using a...

  12. Cherry picker at end of Train Shed with arm fully ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Cherry picker at end of Train Shed with arm fully extended and photographer in bucket - Central of Georgia Railway, Passenger Station & Train Shed, Corner of Louisville (Railroad) Road & West Broad Street, Savannah, Chatham County, GA

  13. Paleoflood investigations for Cherry Creek Basin, Eastern Colorado

    USGS Publications Warehouse

    Jarrett, R.D.

    2004-01-01

    In 1950 when Cherry Creek dam, which is located in Denver. Colorado, was completed, the design flood was 5,126 m3/s. Two recent probable maximum flood (PMF) estimates for the dam range from 14,840 to 18,750 m 3/s demonstrate the uncertainty in estimating extreme flooding in eastern Colorado. PMF difference is due in part to a lack of extreme rainfall and flood data in eastern Colorado. A paleoflood study was conducted to assist dam-safety officials in assessing the risk of large floods in Cherry Creek basin. An envelope curve encompassing maximum contemporary floods (19 sites) and paleofloods (99 sites) was developed for Cherry Creek basin streams; paleoflood data reflect maximum flooding during the last few hundred to many thousands of years. Maximum paleofloods in Cherry Creek range from about 1,050 m 3/s near Franktown (in about 5,000 to at least 10,000 years), about 2,100 m3/s near Melvin (in about 1,500 to 5,000 years), and about 2,270 m3/s at Cherry Creek Reservoir (also in about 1,500 to 5,000 years). Flood-frequency relations for Cherry Creek, which incorporate paleoflood data, indicate the 10,000-year flood (10-4 annual exceedence probability) ranges from about 1,200 m3/s (near Franktown) to about 2,200 m3/s (near Melvin). PMF estimates are about six to eight times larger than paleofloods in Cherry Creek basin. Additional research in flood hydrometeorology is needed to help dam safety officials evaluate potential safety problems related to large floods in Cherry Creek basin. Copyright ASCE 2004.

  14. Seasonal distributions of the western cherry fruit fly (Diptera: Tephritidae) among host and nonhost fruit trees.

    PubMed

    Yee, Wee L

    2014-01-01

    Seasonal distributions of the western cherry fruit fly, Rhagoletis indifferens Curran (Diptera: Tephritidae), in sweet cherry (Prunus avium (L.) L.) (major host), black hawthorn (occasional developmental host) (Crataegus douglasii Lindley), and other trees were determined in a ponderosa pine ecosystem in Washington state, USA. The hypothesis that most fly dispersal from cherry trees occurs after fruit senesce or drop was tested, with emphasis on movement to black hawthorn trees. Sweet cherry fruit developed earlier than black hawthorn, bitter cherry (common host), choke cherry, and apple fruit. Flies were usually captured first in sweet cherry trees but were caught in bitter cherry and other trees throughout the season. Peak fly capture periods in sweet cherry began around the same time or slightly earlier than in other trees. However, peak fly capture periods in black hawthorn and other nonsweet cherry trees continued after peak periods in sweet cherry ended, or relative fly numbers within sweet cherry declined more quickly than those within other trees. Larvae were reared from sweet and bitter cherry but not black hawthorn fruit. Results provide partial support for the hypothesis in that although R. indifferens commonly disperses from sweet cherry trees with fruit, it could disperse more, or more flies are retained in nonsweet cherry trees after than before sweet cherries drop. This could allow opportunities for the flies to use other fruit for larval development. Although R. indifferens infestation in black hawthorn was not detected, early season fly dispersal to this and other trees and fly presence in bitter cherry could make fly management in sweet cherry difficult. PMID:25527581

  15. Combined heat and controlled atmosphere quarantine treatments for control of western cherry fruit fly in sweet cherries.

    PubMed

    Neven, Lisa G; Rehfield-Ray, Linda

    2006-06-01

    Nonchemical quarantine treatments, using a combination of short duration high temperatures under low oxygen, elevated carbon dioxide atmospheric environment were developed to control western cherry fruit fly, Rhagoletis indifferens Curran, in sweet cherries, Prunus avium (L.). The two treatments developed use a chamber temperature of 45 degrees C for 45 min and a chamber temperature of 47 degreesd C for 25 min, both under a 1% oxygen, 15% carbon dioxide, -2 degrees C dew point environment. Both these treatments have been shown to provide control of all life stages of western cherry fruit fly while preserving commodity market quality. There was no definitive egg or larval stage, which was demonstrated to be the most tolerant to either controlled atmosphere temperature treatment system treatment. Efficacy tests for both treatments resulted in 100% mortality of >5000 western cherry fruit flies in each treatment. These treatments may provide, with further study, quarantine security in exported sweet cherries where western cherry fruit fly is a quarantine concern and fumigation with methyl bromide is not desired. PMID:16813295

  16. Flower constancy in insect pollinators

    PubMed Central

    Ratnieks, Francis L.W.

    2011-01-01

    As first noted by Aristotle in honeybee workers, many insect pollinators show a preference to visit flowers of just one species during a foraging trip. This “flower constancy” probably benefits plants, because pollen is more likely to be deposited on conspecific stigmas. But it is less clear why insects should ignore rewarding alternative flowers. Many researchers have argued that flower constancy is caused by constraints imposed by insect nervous systems rather than because flower constancy is itself an efficient foraging method. We argue that this view is unsatisfactory because it both fails to explain why foragers flexibly adjust the degree of flower constancy and does not explain why foragers of closely related species show different degrees of constancy. While limitations of the nervous system exist and are likely to influence flower constancy to some degree, the observed behavioural flexibility suggests that flower constancy is a successful foraging strategy given the insect’s own information about different foraging options. PMID:22446521

  17. Origins of flower morphology.

    PubMed

    Endress, P K

    2001-08-15

    Flowers evolved in many steps, probably starting long before flowering plants (angiophytes) originated. Certain parts of flowers are conservative and have not changed much during evolution; others are evolutionarily highly plastic. Here conservative features are discussed and an attempt is made to trace them back through their evolutionary history. Microsporangia and ovules (which develop into seeds) are preangiophyte floral elements. Angiospermy, combined with postgenital fusion, was the most prominent key innovation in angiophytes. Angiospermy and thecal organization of stamens originated earlier than all clades of extant angiosperms (the crown group of angiophytes). Differentiation of a perianth into calyx and corolla and syncarpy appeared after the first branching of the basalmost clades of extant angiosperms. Sympetaly and floral tubes as well as tenuinucellar, unitegmic ovules originated as major innovations in the clade that led to asterids. An obvious trend in flower evolution is increased synorganisation of parts, which led to new structures. Fixation of floral organ number and position was a precondition for synorganization. Concomitantly, plasticity changed from number and position of organs to shape of the new structures. Character distribution mapped onto cladograms indicates that key innovations do not appear suddenly, but start with trials and only later become deeply rooted genetically in the organization. This is implied from the common occurrence of reversals in the early history of an innovation. J. Exp. Zool. (Mol. Dev. Evol.) 291:105-115, 2001. PMID:11479912

  18. Identification of chilling and heat requirements of cherry trees--a statistical approach.

    PubMed

    Luedeling, Eike; Kunz, Achim; Blanke, Michael M

    2013-09-01

    Most trees from temperate climates require the accumulation of winter chill and subsequent heat during their dormant phase to resume growth and initiate flowering in the following spring. Global warming could reduce chill and hence hamper the cultivation of high-chill species such as cherries. Yet determining chilling and heat requirements requires large-scale controlled-forcing experiments, and estimates are thus often unavailable. Where long-term phenology datasets exist, partial least squares (PLS) regression can be used as an alternative, to determine climatic requirements statistically. Bloom dates of cherry cv. 'Schneiders späte Knorpelkirsche' trees in Klein-Altendorf, Germany, from 24 growing seasons were correlated with 11-day running means of daily mean temperature. Based on the output of the PLS regression, five candidate chilling periods ranging in length from 17 to 102 days, and one forcing phase of 66 days were delineated. Among three common chill models used to quantify chill, the Dynamic Model showed the lowest variation in chill, indicating that it may be more accurate than the Utah and Chilling Hours Models. Based on the longest candidate chilling phase with the earliest starting date, cv. 'Schneiders späte Knorpelkirsche' cherries at Bonn exhibited a chilling requirement of 68.6 ± 5.7 chill portions (or 1,375 ± 178 chilling hours or 1,410 ± 238 Utah chill units) and a heat requirement of 3,473 ± 1,236 growing degree hours. Closer investigation of the distinct chilling phases detected by PLS regression could contribute to our understanding of dormancy processes and thus help fruit and nut growers identify suitable tree cultivars for a future in which static climatic conditions can no longer be assumed. All procedures used in this study were bundled in an R package ('chillR') and are provided as Supplementary materials. The procedure was also applied to leaf emergence dates of walnut (cv. 'Payne') at Davis, California. PMID

  19. Complete nucleotide sequence of a virus associated with rusty mottle disease of sweet cherry (Prunus avium).

    PubMed

    Villamor, D V; Druffel, K L; Eastwell, K C

    2013-08-01

    Cherry rusty mottle is a disease of sweet cherries first described in 1940 in western North America. Because of the graft-transmissible nature of the disease, a viral nature of the disease was assumed. Here, the complete genomic nucleotide sequences of virus isolates from two trees expressing cherry rusty mottle disease symptoms are characterized; the virus is designated cherry rusty mottle associated virus (CRMaV). The biological and molecular characteristics of this virus in comparison to those of cherry necrotic rusty mottle virus (CNRMV) and cherry green ring mottle virus (CGRMV) are described. CRMaV was subsequently detected in additional sweet cherry trees expressing symptoms of cherry rusty mottle disease. PMID:23525699

  20. Reinspiring Japanese Educational Objectives.

    ERIC Educational Resources Information Center

    Wada, Shuji

    1993-01-01

    Provides a brief overview of the history of modern Japanese education, its early modernization, and the policy of intertwining the Japanese ideology with Western technology. Proposes the establishment of a new Buddhist-inspired philosophy of education. (GLR)

  1. Single-molecule localization microscopy using mCherry.

    PubMed

    Winterflood, Christian M; Ewers, Helge

    2014-11-10

    We demonstrate the potential of the commonly used red fluorescent protein mCherry for single-molecule super-resolution imaging. mCherry can be driven into a light-induced dark state in the presence of a thiol from which it can recover spontaneously or by irradiation with near UV light. We show imaging of subcellular protein structures such as microtubules and the nuclear pore complex with a resolution below 40 nm. We were able to image the C-terminus of the nuclear pore protein POM121, which is on the inside of the pore and not readily accessible for external labeling. The photon yield for mCherry is comparable to that of the latest optical highlighter fluorescent proteins. Our findings show that the widely used mCherry red fluorescent protein and the vast number of existing mCherry fusion proteins are readily amenable to super-resolution imaging. This obviates the need for generating novel protein fusions that may compromise function or the need for external fluorescent labeling. PMID:25111075

  2. 7 CFR 930.29 - Eligibility for membership on Cherry Industry Administrative Board.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Eligibility for membership on Cherry Industry... Cherry Industry Administrative Board. (a) Each grower member and each grower alternate member of the... shall be prohibited from having any financial interest in the cherry industry and shall possess...

  3. 7 CFR 52.784 - Score sheet for canned red tart pitted cherries.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Score sheet for canned red tart pitted cherries. 52.784 Section 52.784 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING... Pitted Cherries 1 Score Sheet § 52.784 Score sheet for canned red tart pitted cherries. Size and kind...

  4. 7 CFR 930.29 - Eligibility for membership on Cherry Industry Administrative Board.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Eligibility for membership on Cherry Industry... AGRICULTURE TART CHERRIES GROWN IN THE STATES OF MICHIGAN, NEW YORK, PENNSYLVANIA, OREGON, UTAH, WASHINGTON... Cherry Industry Administrative Board. (a) Each grower member and each grower alternate member of...

  5. 7 CFR 52.802 - Grades of frozen red tart pitted cherries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Grades of frozen red tart pitted cherries. 52.802... OTHER PROCESSED FOOD PRODUCTS 1 United States Standards for Grades of Frozen Red Tart Pitted Cherries Product Description and Grades § 52.802 Grades of frozen red tart pitted cherries. (a) “U.S. Grade A”...

  6. 7 CFR 52.812 - Score sheet for frozen red tart pitted cherries.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Score sheet for frozen red tart pitted cherries. 52..., AND CERTAIN OTHER PROCESSED FOOD PRODUCTS 1 United States Standards for Grades of Frozen Red Tart Pitted Cherries Score Sheet § 52.812 Score sheet for frozen red tart pitted cherries. Size and kind...

  7. 7 CFR 52.802 - Grades of frozen red tart pitted cherries.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Grades of frozen red tart pitted cherries. 52.802... OTHER PROCESSED FOOD PRODUCTS 1 United States Standards for Grades of Frozen Red Tart Pitted Cherries Product Description and Grades § 52.802 Grades of frozen red tart pitted cherries. (a) “U.S. Grade A”...

  8. 7 CFR 52.802 - Grades of frozen red tart pitted cherries.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 2 2013-01-01 2013-01-01 false Grades of frozen red tart pitted cherries. 52.802... of Frozen Red Tart Pitted Cherries Product Description and Grades § 52.802 Grades of frozen red tart pitted cherries. (a) “U.S. Grade A” (or “U.S. Fancy”) is the quality of frozen red tart pitted...

  9. 7 CFR 52.802 - Grades of frozen red tart pitted cherries.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 2 2014-01-01 2014-01-01 false Grades of frozen red tart pitted cherries. 52.802... of Frozen Red Tart Pitted Cherries Product Description and Grades § 52.802 Grades of frozen red tart pitted cherries. (a) “U.S. Grade A” (or “U.S. Fancy”) is the quality of frozen red tart pitted...

  10. 7 CFR 52.812 - Score sheet for frozen red tart pitted cherries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Score sheet for frozen red tart pitted cherries. 52..., AND CERTAIN OTHER PROCESSED FOOD PRODUCTS 1 United States Standards for Grades of Frozen Red Tart Pitted Cherries Score Sheet § 52.812 Score sheet for frozen red tart pitted cherries. Size and kind...

  11. 7 CFR 52.812 - Score sheet for frozen red tart pitted cherries.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Score sheet for frozen red tart pitted cherries. 52..., AND CERTAIN OTHER PROCESSED FOOD PRODUCTS 1 United States Standards for Grades of Frozen Red Tart Pitted Cherries Score Sheet § 52.812 Score sheet for frozen red tart pitted cherries. Size and kind...

  12. 7 CFR 52.802 - Grades of frozen red tart pitted cherries.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Grades of frozen red tart pitted cherries. 52.802... OTHER PROCESSED FOOD PRODUCTS 1 United States Standards for Grades of Frozen Red Tart Pitted Cherries Product Description and Grades § 52.802 Grades of frozen red tart pitted cherries. (a) “U.S. Grade A”...

  13. 7 CFR 930.29 - Eligibility for membership on Cherry Industry Administrative Board.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Eligibility for membership on Cherry Industry... AGRICULTURE TART CHERRIES GROWN IN THE STATES OF MICHIGAN, NEW YORK, PENNSYLVANIA, OREGON, UTAH, WASHINGTON... Cherry Industry Administrative Board. (a) Each grower member and each grower alternate member of...

  14. 33 CFR 208.82 - Hetch Hetchy, Cherry Valley, and Don Pedro Dams and Reservoirs.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false Hetch Hetchy, Cherry Valley, and..., Cherry Valley, and Don Pedro Dams and Reservoirs. The Turlock Irrigation District and Modesto Irrigation..., shall operate Hetch Hetchy Dam and Reservoir and Cherry Valley Dam and Reservoir in the interest...

  15. 7 CFR 52.784 - Score sheet for canned red tart pitted cherries.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 2 2014-01-01 2014-01-01 false Score sheet for canned red tart pitted cherries. 52... for Grades of Canned Red Tart Pitted Cherries 1 Score Sheet § 52.784 Score sheet for canned red tart pitted cherries. Size and kind of container Container mark or identification Label Net weight...

  16. 7 CFR 52.784 - Score sheet for canned red tart pitted cherries.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 2 2013-01-01 2013-01-01 false Score sheet for canned red tart pitted cherries. 52... for Grades of Canned Red Tart Pitted Cherries 1 Score Sheet § 52.784 Score sheet for canned red tart pitted cherries. Size and kind of container Container mark or identification Label Net weight...

  17. 7 CFR 930.29 - Eligibility for membership on Cherry Industry Administrative Board.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Eligibility for membership on Cherry Industry... AGRICULTURE TART CHERRIES GROWN IN THE STATES OF MICHIGAN, NEW YORK, PENNSYLVANIA, OREGON, UTAH, WASHINGTON... Cherry Industry Administrative Board. (a) Each grower member and each grower alternate member of...

  18. 7 CFR 52.784 - Score sheet for canned red tart pitted cherries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Score sheet for canned red tart pitted cherries. 52.784 Section 52.784 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING... Pitted Cherries 1 Score Sheet § 52.784 Score sheet for canned red tart pitted cherries. Size and kind...

  19. 7 CFR 52.784 - Score sheet for canned red tart pitted cherries.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Score sheet for canned red tart pitted cherries. 52.784 Section 52.784 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING... Pitted Cherries 1 Score Sheet § 52.784 Score sheet for canned red tart pitted cherries. Size and kind...

  20. 33 CFR 208.82 - Hetch Hetchy, Cherry Valley, and Don Pedro Dams and Reservoirs.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Hetch Hetchy, Cherry Valley, and..., Cherry Valley, and Don Pedro Dams and Reservoirs. The Turlock Irrigation District and Modesto Irrigation..., shall operate Hetch Hetchy Dam and Reservoir and Cherry Valley Dam and Reservoir in the interest...

  1. 76 FR 46651 - Sweet Cherries Grown in Designated Counties in Washington; Continuance Referendum

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ...; ] DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 923 Sweet Cherries Grown in Designated... sweet cherry growers to determine whether they favor continuance of the marketing order regulating the handling of sweet cherries grown in designated counties in Washington. DATES: The referendum will...

  2. 7 CFR 930.29 - Eligibility for membership on Cherry Industry Administrative Board.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Eligibility for membership on Cherry Industry... AGRICULTURE TART CHERRIES GROWN IN THE STATES OF MICHIGAN, NEW YORK, PENNSYLVANIA, OREGON, UTAH, WASHINGTON... Cherry Industry Administrative Board. (a) Each grower member and each grower alternate member of...

  3. 75 FR 10442 - Sweet Cherries Grown in Designated Counties in Washington; Change in the Handling Regulation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-08

    ...; ] DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 923 Sweet Cherries Grown in Designated Counties in Washington; Change in the Handling Regulation AGENCY: Agricultural Marketing Service, USDA... currently prescribed for cherries under the Washington cherry marketing order. The marketing order...

  4. 76 FR 65357 - Tart Cherries Grown in Michigan, New York, Pennsylvania, Oregon, Utah, Washington, and Wisconsin...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-21

    ...This rule changes the grower diversion regulations prescribed under the marketing order for tart cherries (order). The order regulates the handling of tart cherries grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah, Washington, and Wisconsin and is administered locally by the Cherry Industry Administrative Board (Board). This rule suspends indefinitely the regulations......

  5. The Japanese Mind: Understanding Contemporary Japanese Culture.

    ERIC Educational Resources Information Center

    Davies, Roger J., Ed.; Ikeno, Osamu, Ed.

    This collection of essays offers an overview of contemporary Japanese culture, and can serve as a resource for classes studying Japan. The 28 essays offer an informative, accessible look at the values, attitudes, behavior patterns, and communication styles of modern Japan from the unique perspective of the Japanese people. Filled with examples…

  6. Japanese Radio Exercises. Revised.

    ERIC Educational Resources Information Center

    Young, Jocelyn

    This unit focuses on Japanese radio exercises which became popular in Japan just after World War II and are still used among students and workers in companies to help raise morale and form group unity. The exercises reflect the general role of exercise in Japanese culture--to serve as a symbol of unity and cooperation among the Japanese, as well…

  7. Bullying in Japanese Schools.

    ERIC Educational Resources Information Center

    Kobayashi, Futoshi

    Noting that although many Western educators praise the Japanese educational system because of its students' academic achievements, schools in Japan have developed severe and prevalent problems with student bullying. This paper examines the problem of bullying in Japanese schools. Part 1 of the paper reviews bullying incidents in Japanese schools…

  8. Comparative Freezing Patterns in Stems of Cherry and Azalea 1

    PubMed Central

    Dennis, Frank G.; Lumis, Glen P.; Olien, C. Robert

    1972-01-01

    Ice formation in stems, as determined by means of an electrophoretic mobility technique, occurs much more rapidly in azalea than in sour cherry. The difference is more marked in the bark than in the wood. Disrupting the structure of the tissues completely eliminates differences in freezing patterns, although gross anatomical differences do not appear to account for differences in species response. Microscopic examination of frozen stems indicated that little redistribution of water occurred during freezing in azalea, and the tissues were disrupted as these crystals developed. In cherry, on the other hand, water diffused to nucleating centers where crystal growth was not opposed, giving rise to “glaciers.” PMID:16658210

  9. Determination of some B Vitamins in Sour Cherry Juice Using Dispersive Liquid-liquid Microextraction Followed by High-performance Liquid Chromatography.

    PubMed

    Parsaei, Parvin; Bahmaei, Manouchehr; Ghannadi, AliReza

    2014-01-01

    Dispersive liquid-liquid microextraction method (DLLME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in sour cherry juice. This method was rapid, simple and sensitive. Separation was accomplished using a C18 column. The optimum chromatographic conditions were found to be: mobile phase consisted of 8% methanol and 92% aqueous phase (1% (V/V) acetic acid water solution); flow rate, 0.7 mL/min; detection wavelength, 260 nm and pH, 3.3. The extraction efficiency of thiamine, nicotinamide and pyridoxine was influenced by factors such as: additional salt effect, the kind and volume of disperser and extraction solvents. In this research, the limit of detection (LOD) and quantification (LOQ) were 0.9 and 3 ng/mL for thiamine, 1.5 and 5 ng/mL for nicotinamide, 0.9 and 3 ng/mL for pyridoxine. The relative standard deviations (RSDs) were less than 2.87% (n=3). An appropriate linear behavior over the observed concentration range was obtained with the value of R²>0.996 for the target vitamins. This method was successfully applied to the sour cherry juice samples. Sour cherry var. Gise (Prunus cerasus var. Gise), which was used in this research, was a local variety of the sour cherry with large stone, double flowers, double fruits, dark red skin and dark red juice. This variety was identified in high altitude areas of Isfahan province after five years of study, since 2005, by Agricultural and Natural Resources Research Center of Isfahan. PMID:25587335

  10. Determination of some B Vitamins in Sour Cherry Juice Using Dispersive Liquid-liquid Microextraction Followed by High-performance Liquid Chromatography

    PubMed Central

    Parsaei, Parvin; Bahmaei, Manouchehr; Ghannadi, AliReza

    2014-01-01

    Dispersive liquid-liquid microextraction method (DLLME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in sour cherry juice. This method was rapid, simple and sensitive. Separation was accomplished using a C18 column. The optimum chromatographic conditions were found to be: mobile phase consisted of 8% methanol and 92% aqueous phase (1% (V/V) acetic acid water solution); flow rate, 0.7 mL/min; detection wavelength, 260 nm and pH, 3.3. The extraction efficiency of thiamine, nicotinamide and pyridoxine was influenced by factors such as: additional salt effect, the kind and volume of disperser and extraction solvents. In this research, the limit of detection (LOD) and quantification (LOQ) were 0.9 and 3 ng/mL for thiamine, 1.5 and 5 ng/mL for nicotinamide, 0.9 and 3 ng/mL for pyridoxine. The relative standard deviations (RSDs) were less than 2.87% (n=3). An appropriate linear behavior over the observed concentration range was obtained with the value of R²>0.996 for the target vitamins. This method was successfully applied to the sour cherry juice samples. Sour cherry var. Gise (Prunus cerasus var. Gise), which was used in this research, was a local variety of the sour cherry with large stone, double flowers, double fruits, dark red skin and dark red juice. This variety was identified in high altitude areas of Isfahan province after five years of study, since 2005, by Agricultural and Natural Resources Research Center of Isfahan. PMID:25587335

  11. Predicting the timing of cherry blossoms in Washington, DC and Mid-Atlantic States in response to climate change.

    PubMed

    Chung, Uran; Mack, Liz; Yun, Jin I; Kim, Soo-Hyung

    2011-01-01

    Cherry blossoms, an icon of spring, are celebrated in many cultures of the temperate region. For its sensitivity to winter and early spring temperatures, the timing of cherry blossoms is an ideal indicator of the impacts of climate change on tree phenology. Here, we applied a process-based phenology model for temperate deciduous trees to predict peak bloom dates (PBD) of flowering cherry trees (Prunus×yedoensis 'Yoshino' and Prunus serrulata 'Kwanzan') in the Tidal Basin, Washington, DC and the surrounding Mid-Atlantic States in response to climate change. We parameterized the model with observed PBD data from 1991 to 2010. The calibrated model was tested against independent datasets of the past PBD data from 1951 to 1970 in the Tidal Basin and more recent PBD data from other locations (e.g., Seattle, WA). The model performance against these independent data was satisfactory (Yoshino: r(2) = 0.57, RMSE = 6.6 days, bias = 0.9 days and Kwanzan: r(2) = 0.76, RMSE = 5.5 days, bias = -2.0 days). We then applied the model to forecast future PBD for the region using downscaled climate projections based on IPCC's A1B and A2 emissions scenarios. Our results indicate that PBD at the Tidal Basin are likely to be accelerated by an average of five days by 2050 s and 10 days by 2080 s for these cultivars under a mid-range (A1B) emissions scenario projected by ECHAM5 general circulation model. The acceleration is likely to be much greater (13 days for 2050 s and 29 days for 2080s) under a higher (A2) emissions scenario projected by CGCM2 general circulation model. Our results demonstrate the potential impacts of climate change on the timing of cherry blossoms and illustrate the utility of a simple process-based phenology model for developing adaptation strategies to climate change in horticulture, conservation planning, restoration and other related disciplines. PMID:22087317

  12. Occupational allergy caused by flowers.

    PubMed

    de Jong, N W; Vermeulen, A M; Gerth van Wijk, R; de Groot, H

    1998-02-01

    We describe 14 consecutive patients with complaints due to the handling of flowers. The symptoms varied from allergic rhinoconjunctivitis and asthma to urticaria. Most patients had professions in the flower industry. Skin prick tests (SPT) were performed with home-made pollen extracts from 17 different flowers known to be the most commonly grown and sold in The Netherlands RAST against mugwort, chrysanthemum, and solidago was performed. The diagnosis of atopy against flowers was based on work-related symptoms due to the handling of flowers, positive SPT with flower extracts, and positive RAST. The concordance between SPT and case history was 74%, and that between SPT and RAST was 77% Extensive cross-sensitization was seen to pollen of several members of the Compositae family (e.g., Matricaria, chrysanthemum, solidago) and to pollen of the Amaryllidaceae family (Alstroemeria and Narcissus). Homemade flower extracts can be used to confirm IgE-mediated flower allergy. Mugwort can be used as a screening test for possible flower allergy. For most patients, the allergy led to a change of profession. PMID:9534922

  13. Flowers that threaten Funza.

    PubMed

    Kendall, S

    1993-01-01

    Water shortages have resulted from agricultural development in a rural area outside Bogota, Colombia. These shortages have increased women's work load and caused problems in managing households because the water must be boiled before ingestion. In the community of Funza, women must obtain clean water in buckets at night from the main valve, which has insufficient water pressure and a slow stream. Some barrios collect water on a weekly basis. The local restaurant in town obtains water once a week from a tanker; the town is lucky to receive water three times a week. Men assume that women will take care of the problem. The mayor says that the piped water from Bogota will soon be connected and that each barrio will have its own valve. Women are concerned that the supply, even with new valves, will be limited and mixed with dirty lagoon water. Experts are saying that the water shortage and quality problems that began seven years ago will lead to rationing within three to six years. The flower companies, that came to the area 22 years age, are blamed for the water problems. People say that the flower companies have piped clean water from the area's supply in the San Patricia and that underground sources of water have been used up as well. The industry provides jobs and income, which have improved the standard of living, but there is little consideration given to the water supply. The community shifted water sources to the lagoon at a time when the water was being contaminated by sewage and pesticides and chemicals from the flower companies. PMID:12287011

  14. Cherry Fruitworm, an emerging pest of Mississippi blueberries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The cherry fruitworm (CFW) is a univoltine moth, native to the U.S., and whose larvae preferentially infest rosaceous and ericaceous fruits. CFW larvae have been confirmed infesting rabbiteye blueberries in Mississippi, and this typically northern pest’s appearance may represent a new State record. ...

  15. First Complete Genome Sequence of Cherry virus A.

    PubMed

    Koinuma, Hiroaki; Nijo, Takamichi; Iwabuchi, Nozomu; Yoshida, Tetsuya; Keima, Takuya; Okano, Yukari; Maejima, Kensaku; Yamaji, Yasuyuki; Namba, Shigetou

    2016-01-01

    The 5'-terminal genomic sequence of Cherry virus A (CVA) has long been unknown. We determined the first complete genome sequence of an apricot isolate of CVA (7,434 nucleotides [nt]). The 5'-untranslated region was 107 nt in length, which was 53 nt longer than those of known CVA sequences. PMID:27284130

  16. Wheat Lines Resistant to Bird Cherry-Oat Aphid, 2006

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bird cherry-oat aphid (BCOA) is one of the most common aphid pests of wheat worldwide, and resistant lines are needed as alternatives to chemical control. Several entries of wheat were evaluated against BCOA in a growth chamber test. The number of nymphs deposited by winged BCOA in the first 24 h ...

  17. First Complete Genome Sequence of Cherry virus A

    PubMed Central

    Koinuma, Hiroaki; Nijo, Takamichi; Iwabuchi, Nozomu; Yoshida, Tetsuya; Keima, Takuya; Okano, Yukari; Maejima, Kensaku; Yamaji, Yasuyuki

    2016-01-01

    The 5′-terminal genomic sequence of Cherry virus A (CVA) has long been unknown. We determined the first complete genome sequence of an apricot isolate of CVA (7,434 nucleotides [nt]). The 5′-untranslated region was 107 nt in length, which was 53 nt longer than those of known CVA sequences. PMID:27284130

  18. Screening for Bird cherry-oat aphid resistance in barley

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bird cherry-oat aphid (BCOA), Rhopalosiphum padi (L.), has been reported to cause yield loss in small grains both through its role as an efficient vector of the PAV strain of Barley yellow dwarf virus (BYDV) and by actual feeding damage to winter and spring small grains by aviruliferous BCOAs. Barl...

  19. Screening for bird cherry-oat aphid resistance in barley

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bird cherry-oat aphid (BCOA), Rhopalosiphum padi (L.), has been reported to cause yield loss in small grains both through its role as an efficient vector of the PAV strain of Barley yellow dwarf virus (BYDV) and by actual feeding damage to winter and spring small grains by aviruliferous BCOAs. Barl...

  20. 21 CFR 145.126 - Artificially sweetened canned cherries.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Artificially sweetened canned cherries. 145.126 Section 145.126 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION CANNED FRUITS Requirements for Specific Standardized Canned...

  1. 21 CFR 145.126 - Artificially sweetened canned cherries.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Artificially sweetened canned cherries. 145.126 Section 145.126 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION CANNED FRUITS Requirements for Specific Standardized Canned...

  2. 21 CFR 145.126 - Artificially sweetened canned cherries.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Artificially sweetened canned cherries. 145.126 Section 145.126 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION CANNED FRUITS Requirements for Specific Standardized Canned...

  3. 7 CFR 923.322 - Washington cherry handling regulation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... List of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and on... solids requirements. (d) (e) Pack. (1) When containers of cherries are marked with a row count/row size... provisions of paragraphs (a), (b), (c), and (e) of this section, and of §§ 923.41 and 923.55. (1)...

  4. Bird cherry-oat aphid: do we have resistance?

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bird cherry-oat aphid (BCOA), Rhopalosiphum padi (L.), is a highly efficient, non-propagative, persistent vector of the phloem limited leutovirus BYD-PAV. BYD is the most important viral disease of cereal grains in the world and PAV is the most prevalent strain of BYD in North America. Not all BCO...

  5. Triticale Lines Resistant to Bird Cherry-Oat Aphid, 2006

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bird cherry-oat aphid (BCOA) is one of the most common aphid pests of small grains worldwide. Triticale is a promising source of resistance to BCOA that may be developed further or crossed with wheat to transfer resistance to that crop. Several entries of triticale were evaluated against BCOA in a...

  6. Bird cherry-oat aphid resistance in barley

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bird cherry-oat aphid (BCOA), Rhopalosiphum padi (L.), has been reported to cause yield loss in small grains both through its role as an efficient vector of the PAV strain of barley yellow dwarf virus (BYDV) and by actual feeding damage to winter and spring small grains by aviruliferous BCOAs. Barl...

  7. Screening for bird cherry-oat aphid resistance in barley

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bird cherry-oat aphid (BCOA), Rhopalosiphum padi (L.), has been reported to cause yield loss in small grains both through its role as an efficient vector of the PAV strain of barley yellow dwarf virus (BYDV) and by actual feeding damage to winter and spring small grains by aviruliferous BCOAs. Barl...

  8. Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China.

    PubMed

    Wang, Jiawei; Zhu, Dongzi; Tan, Yue; Zong, Xiaojuan; Wei, Hairong; Hammond, Rosemarie W; Liu, Qingzhong

    2016-03-01

    Little cherry virus 1 (LChV-1), associated with little cherry disease (LCD), has a significant impact on fruit quality of infected sweet cherry trees. We report the full genome sequence of an isolate of LChV-1 from Taian, China (LChV-1-TA), detected by small-RNA deep sequencing and amplified by overlapping RT-PCR. The LChV-1-TA genome was 16,932 nt in length and contained nine open reading frames (ORFs), with sequence identity at the overall genome level of 76%, 76%, and 78% to LChV-1 isolates Y10237 (UW2 isolate), EU715989 (ITMAR isolate) and JX669615 (V2356 isolate), respectively. Based on the phylogenetic analysis of HSP70h amino acid sequences of Closteroviridae family members, LChV-1-TA was grouped into a well-supported cluster with the members of the genus Velarivirus and was also closely related to other LChV-1 isolates. This is the first report of the complete nucleotide sequence of LChV-1 infecting sweet cherry in China. PMID:26733294

  9. Stop and Paint the Flowers.

    ERIC Educational Resources Information Center

    Phillips, Shelley

    2002-01-01

    Describes an art lesson where students used watercolors to paint a flower bouquet arranged in a vase. Explains that the students viewed examples of flower bouquets by artists such as Vincent van Gogh and Odilon Redon. Discusses, in detail, the process of creating the artworks. (CMK)

  10. Extensive Reading in Japanese

    ERIC Educational Resources Information Center

    Hitosugi, Claire Ikumi; Day, Richard R.

    2004-01-01

    This article discusses how we incorporated an extensive reading (ER) program into a second semester Japanese course at the University of Hawai'i using Japanese children's literature. After summarizing the ten principles of ER, we describe how we addressed six critical issues faced while introducing ER into the course. We also discuss the outcomes…

  11. The Japanese containerless experiments

    NASA Technical Reports Server (NTRS)

    Azuma, Hisao

    1990-01-01

    There are three sets of Japanese containerless experiments. The first is Drop dynamics research. It consists of acoustic levitation and large amplitude drop oscillation. The second is Optical materials processing in an acoustic levitation furnace. And the third is Electrostatic levitator development by two different Japanese companies.

  12. The Japanese American Story.

    ERIC Educational Resources Information Center

    Fukei, Budd

    This book presents a view of the Japanese American experience from the time of their immigration to this country in the 1800s to their acculturation into American society in the 1970s. Topics dealt with include the prejudice and mistrust experienced by the Japanese immigrants in this country, particularly their evacuation and internment in…

  13. Japanese Media in English.

    ERIC Educational Resources Information Center

    Tanaka, Sachiko Oda

    1995-01-01

    Describes the use of English in the media in Japan, focusing on the role and history of English-language newspapers, radio, and television programs, as well as the proliferation of English-language films shown in Japanese cinemas. Discusses the implications of English in the Japanese media. (20 references) (MDM)

  14. Japanese Quality Control Circles.

    ERIC Educational Resources Information Center

    Nishiyama, Kazuo

    In recent years, United States scholars with an interest in international business and organizational communication have begun to notice the success of Japanese "quality control circles." These are small groups, usually composed of seven to ten workers, who are organized at the production levels within most large Japanese factories. A typical…

  15. Japanese Elementary School Education.

    ERIC Educational Resources Information Center

    Stevenson, Harold W.

    1991-01-01

    Discusses the development of Japanese elementary education in the context of three periods of its history. Considers salient characteristics of Japanese elementary schools and teaching procedures; these include curriculum; social and moral education; classroom environment; teachers; afterschool classes; college entrance examinations; the kyoiku…

  16. Determination of self-incompatibility groups of sweet cherry genotypes from Turkey.

    PubMed

    Ipek, A; Gulen, H; Akcay, M E; Ipek, M; Ergin, S; Eris, A

    2011-01-01

    Determination of S-allele combinations of sweet cherry genotypes and cultivars has importance for both growers and breeders. We determined S-allele combinations of 40 local Turkish sweet cherry genotypes using a PCR-based method. Ten different S-alleles were detected. Although the most common S-allele was S3, as also found in Western genotypes and cultivars, there were some differences in the frequencies of some S-alleles between Turkish and Western sweet cherry genotypes. According to their S-allele compositions, 30 local Turkish sweet cherry genotypes were assigned to 10 previously identified incompatibility groups. For the remaining genotypes, whose S-allele combinations did not fit to any previous incompatibility groups, three more incompatibility groups, XLII, XLIII and XLIV, were proposed. Results obtained from this study will help both sweet cherry growers and breeders to better manage these local Turkish sweet cherry genotypes in their orchards. PMID:21341217

  17. Say it with flowers: Flowering acceleration by root communication.

    PubMed

    Falik, Omer; Hoffmann, Ishay; Novoplansky, Ariel

    2014-01-01

    The timing of reproduction is a critical determinant of fitness, especially in organisms inhabiting seasonal environments. Increasing evidence suggests that inter-plant communication plays important roles in plant functioning. Here, we tested the hypothesis that flowering coordination can involve communication between neighboring plants. We show that soil leachates from Brassica rapa plants growing under long-day conditions accelerated flowering and decreased allocation to vegetative organs in target plants growing under non-inductive short-day conditions. The results suggest that besides endogenous signaling and external abiotic cues, flowering timing may involve inter-plant communication, mediated by root exudates. The study of flowering communication is expected to illuminate neglected aspects of plant reproductive interactions and to provide novel opportunities for controlling the timing of plant reproduction in agricultural settings. PMID:25764422

  18. Say it with flowers: flowering acceleration by root communication.

    PubMed

    Falik, Omer; Hoffmann, Ishay; Novoplansky, Ariel

    2014-01-01

    The timing of reproduction is a critical determinant of fitness, especially in organisms inhabiting seasonal environments. Increasing evidence suggests that inter-plant communication plays important roles in plant functioning. Here, we tested the hypothesis that flowering coordination can involve communication between neighboring plants. We show that soil leachates from Brassica rapa plants growing under long-day conditions accelerated flowering and decreased allocation to vegetative organs in target plants growing under non-inductive short-day conditions. The results suggest that besides endogenous signaling and external abiotic cues, flowering timing may involve inter-plant communication, mediated by root exudates. The study of flowering communication is expected to illuminate neglected aspects of plant reproductive interactions and to provide novel opportunities for controlling the timing of plant reproduction in agricultural settings. PMID:24598343

  19. Epidemiological studies are like cherries, one draws another.

    PubMed

    Lunet, Nuno

    2009-01-01

    The proverb "Words are like cherries", meaning that when you start talking subjects pop up and you end up with long conversations, just like cherries coming out of the plate in chains when you pick one, may also be applied to epidemiological research. A sequence of epidemiological studies, each being drawn from the previous, is presented as an example of how each investigation may raise new questions to be addressed in following studies. This description stresses the need for appropriate planning and the usefulness of pilot testing to depict inadequacies that can hardly be anticipated without field work. I intend to illustrate how epidemiological research can provide a deep approach to research questions, as long as findings are properly interpreted and suboptimal methodological options are taken into account in future investigations. PMID:19713008

  20. The slow and fast pyrolysis of cherry seed.

    PubMed

    Duman, Gozde; Okutucu, Cagdas; Ucar, Suat; Stahl, Ralph; Yanik, Jale

    2011-01-01

    The slow and fast pyrolysis of cherry seeds (CWS) and cherry seeds shells (CSS) was studied in fixed-bed and fluidized bed reactors at different pyrolysis temperatures. The effects of reactor type and temperature on the yields and composition of products were investigated. In the case of fast pyrolysis, the maximum bio-oil yield was found to be about 44 wt% at pyrolysis temperature of 500 °C for both CWS and CSS, whereas the bio yields were of 21 and 15 wt% obtained at 500 °C from slow pyrolysis of CWS and CSS, respectively. Both temperature and reactor type affected the composition of bio-oils. The results showed that bio-oils obtained from slow pyrolysis of CWS and CSS can be used as a fuel for combustion systems in industry and the bio-oil produced from fast pyrolysis can be evaluated as a chemical feedstock. PMID:20801019

  1. Molecular Diagnosis of Subcutaneous Spirometra erinaceieuropaei Sparganosis in a Japanese Immigrant

    PubMed Central

    Tappe, Dennis; Berger, Luise; Haeupler, Alexandra; Muntau, Birgit; Racz, Paul; Harder, Yves; Specht, Katja; Prazeres da Costa, Clarissa; Poppert, Sven

    2013-01-01

    We report a case of subcutaneous sparganosis in a 68-year-old female Japanese immigrant in Germany. The patient complained of a painless erythema caudal of the umbilicus with a palpable subcutaneous cherry-sized lump. Polymerase chain reaction on formalin-fixed parasite tissue identified Spirometra erinaceieuropaei as the causative agent; the proliferative form of sparganosis, which is caused by the branching and disseminating Sparganum proliferum, could, thus, be excluded. From the excised sparganum, an immunofluorescence test was established and revealed an antibody response directed against the parasite's tegument. Histological key features of the plerocercoid that facilitate diagnosis with different stains are presented. PMID:23166198

  2. Genomic analyses of cherry rusty mottle group and cherry twisted leaf-associated viruses reveal a possible new genus within the family betaflexiviridae.

    PubMed

    Villamor, D E V; Susaimuthu, J; Eastwell, K C

    2015-03-01

    It is demonstrated that closely related viruses within the family Betaflexiviridae are associated with a number of diseases that affect sweet cherry (Prunus avium) and other Prunus spp. Cherry rusty mottle-associated virus (CRMaV) is correlated with the appearance of cherry rusty mottle disease (CRMD), and Cherry twisted leaf-associated virus (CTLaV) is linked to cherry twisted leaf disease (CTLD) and apricot ringpox disease (ARPD). Comprehensive analysis of previously reported full genomic sequences plus those determined in this study representing isolates of CTLaV, CRMaV, Cherry green ring mottle virus, and Cherry necrotic rusty mottle virus revealed segregation of sequences into four clades corresponding to distinct virus species. High-throughput sequencing of RNA from representative source trees for CRMD, CTLD, and ARPD did not reveal additional unique virus sequences that might be associated with these diseases, thereby further substantiating the association of CRMaV and CTLaV with CRMD and CTLD or ARPD, respectively. Based on comparison of the nucleotide and amino acid sequence identity values, phylogenetic relationships with other triple-gene block-coding viruses within the family Betaflexiviridae, genome organization, and natural host range, a new genus (Robigovirus) is suggested. PMID:25496302

  3. Spirit Has Flower Power

    NASA Technical Reports Server (NTRS)

    2004-01-01

    The Mars Exploration Rover Spirit took this image with its hazard-avoidance camera on sol 86 (March 31, 2004), after the rover's rock abrasion tool had brushed for three minutes on each of six locations on the rock named 'Mazatzal' to create a flower-shaped mosaic.

    The goal for this operation was to create a brushed area big enough for the miniature thermal emission spectrometer to capture within one of its pixels, which are 11 centimeters (4.3 inches) in diameter at the distance between the rock and the instrument. Because the rock abrasion tool creates individual brushed areas only about 5 centimeters (2 inches) in diameter, the team designed this six-location series of tool placements in order to brush 92 percent to 95 percent of the spectrometer's pixel size.

    This operation was only the second time the rock abrasion tool has created a brushing mosaic. The first time was a three-spot brushing on the rock called 'Humphrey.' The brush was originally designed to be used as an aide during full grinding operations, however it has been very effective in brushing the top layer off of dusty martian rocks to allow scientists a multi-depth look into the rocks on Mars.

  4. Modelling of the spring phenological phases of the Silver birch Betula pendula and Bird cherry Padus racemosa in Baltic region

    NASA Astrophysics Data System (ADS)

    Kalvāns, Andis; Kalvāne, Gunta; Bitāne, Māra; Cepīte-Frišfelde, Daiga; Sīle, Tija; Seņņikovs, Juris

    2014-05-01

    The air temperature is the strongest driving factor of the plant development during spring time in moderate climate conditions. However other factors such as the air temperature during the dormancy period and light conditions can play a role as well. The full potential of the recent and historical phenological observation data can be utilised by modelling tools. We have calibrated seven phenological models described in scientific literature to calculate the likely dates leaf unfolding and start of flowering of the Silver birch Betula pendula and bird cherry Padus racemosa (Kalvāns at al, accepted). Phenological observations are derived from voluntary observation network for period 1960-2009 in Latvia. The number of used observations for each phase range from 149 to 172. Air temperature data measured in meteorological stations closest to the corresponding phenological observation sites are obtained from Latvian Environment, Geology and Meteorology Centre. We used 33 random data subsamples for model calibration to produce a range of model coefficients enabling the estimation of the phenological model uncertainty. It is found that the best reproduction of the observational data are obtained using a simple linear degree day model considering daily minimum and maximum temperature and more complex sigmoidal model honouring the need for low temperatures for dormancy release (UniChill, Chuine, 2000). The median calibration base temperature in the degree day model for the silver birch leaf unfolding is 5.6°C and for start of the flowering 6.7°C; for the bird cherry the corresponding base temperatures are 3.2°C and 3.4°C. The calibrated models and air temperature archive data derived from the Danish Meteorological Institute is used to simulate the respective phase onset in the Estonia, Latvia and Lithuania in 2009. Significant regional differences between modelled phase onset times are observed. There is a wide regional variation of the model uncertainty as well

  5. Molecular analysis of East Anatolian traditional plum and cherry accessions using SSR markers.

    PubMed

    Öz, M H; Vurgun, H; Bakir, M; Büyük, İ; Yüksel, C; Ünlü, H M; Çukadar, K; Karadoğan, B; Köse, Ö; Ergül, A

    2013-01-01

    We conducted SSR analyses of 59 accessions, including 29 traditional plum (Prunus domestica), 24 sweet cherry (Prunus avium), and 1 sour cherry (Prunus cerasus) selected from East Anatolian gene sources and 3 plum and 2 cherry reference accessions for molecular characterization and investigation of genetic relationships. Eight SSR loci [1 developed from the apricot (UDAp-404), 4 from the peach (UDP96-010, UDP96-001, UDP96-019, Pchgms1) and 3 from the cherry (UCD-CH13, UCD-CH17, UCD-CH31) genome] for plum accessions and 9 SSR loci [5 developed from the cherry (PS12A02, UCD-CH13, UCD-CH17, UCD-CH31, UCD-CH21), 3 from the peach (Pchgms1, UDP96-001, UDP96-005) and 1 from the plum (CPSCT010) genome] for cherry accessions were used for genetic identification. A total of 66 and 65 alleles were obtained in the genetic analyses of 31 plum and 28 cherry accessions, respectively. The number of alleles revealed by SSR analysis ranged from 4 to 14 alleles per locus, with a mean value of 8.25 in plum accessions, and from 5 to 10 alleles per locus with a mean value of 7.2 in cherry accessions. Only one case of synonym was identified among the cherry accessions, while no case of synonym was observed among the plum accessions. Genomic SSR markers used in discrimination of plum and cherry accessions showed high cross-species transferability in the Prunus genus. Because of their appreciable polymorphism and cross species transferability, the SSR markers that we evaluated in this study will be useful for studies involving fingerprinting of cherry and plum cultivars. PMID:24301792

  6. Oral immunization of animals with transgenic cherry tomatillo expressing HBsAg

    PubMed Central

    Gao, Yi; Ma, Ying; Li, Mei; Cheng, Tong; Li, Shao-Wei; Zhang, Jun; Xia, Ning-Shao

    2003-01-01

    AIM: To investigate the expression of recombinant HBsAg (rHBsAg) in transgenic cherry tomatillo in order to explore the feasibility of producing HBV oral vaccine with cherry tomatillo by animal immune tests. METHODS: The recombinant plant expression vector containing HBsAg gene was constructed. Mediated with Agrobacterium tumefaciens, HBsAg gene was transferred into cotyledons of cherry tomatillo. Transformed cherry tomatillos were obtained through hygromycin delay-selection. Integrated DNA in transgenic cherry tomatillo was confirmed by hygromycin resistance selection, Gus detection, polymerase chain reaction (PCR) and dot blotting analysis. Antigenicity of rHBsAg was examined by ELISA and the immunogenicity of rHBsAg derived from transgenic cherry tomatillo tissues was confirmed by oral feed of transformed tissues to BALB/c mice primed with commercial HBV vaccines. Specific antibody titers in mice’s serum were examined by ELISA every week. RESULTS: By far, 10 positive lines of transgenic cherry tomatillos containing HBsAg gene were obtained. Among different organs of the same transgenic cherry tomatillo, level of rHBsAg expressed in leaves was the highest with the yield up to 300 ng/g fresh weight. And the rHBsAg expression level in fruits was about 10 ng/g fresh weight. In animal immune tests, oral delivery with transgenic tissues to mice primed with commercial vaccine instead of naive mice resulted in significant immune response. CONCLUSION: The result of this animal immune test indicated the rHBsAg derived from transgenic cherry tomatillo possessed normal immunogenicity. This work demonstrated the feasibility to generate oral immunogenic rHBsAg in transgenic cherry tomatillo, and would provide some experimental approach for the production of low-cost oral vaccines using transgenic cherry tomatillo in large scale. PMID:12717845

  7. Does flower phenology mirror the slowdown of global warming?

    PubMed Central

    Jochner, Susanne; Menzel, Annette

    2015-01-01

    Although recent global warming trends in air temperature are not as pronounced as those observed only one decade ago, global mean temperature is still at a very high level. Does plant phenology – which is believed to be a suitable indicator of climate change – respond in a similar way, that is, does it still mirror recent temperature variations? We explored in detail long-term flowering onset dates of snowdrop, cherry, and lime tree and relevant spring temperatures at three sites in Germany (1901–2012) using the Bayesian multiple change-point approach. We investigated whether mean spring temperature changes were amplified or slowed down in the past decade and how plant phenology responded to the most recent temperature changes. Incorporating records with different end points (i.e., 2002 and 2012), we compared differences in trends and inferred possible differences caused by extrapolating phenological and meteorological data. The new multiple-change point approach is characterized by an enhanced structure and greater flexibility compared to the one change point model. However, the highest model probabilities for phenological (meteorological) records were still obtained for the one change point (linear) model. Marked warming trends in the recent decade were only revealed for mean temperatures of March to May, here better described with one or two change point models. In the majority of cases analyzed, changes in temperatures were well mirrored by phenological changes. However, temperatures in March to May were linked to less strongly advancing onset dates for lime tree flowering during the period 1901-2012, pointing to the likely influence of photoperiodic constraints or unfulfilled chilling requirements. Due to the slowdown of temperature increase, analyses conducted on records ending in 2002 demonstrated distinct differences when compared with records ending in 2012. Extrapolation of trends could therefore (along with the choice of the statistical method

  8. How the Japanese work.

    PubMed

    Chambers, D W

    1998-01-01

    The Japanese do not work harder or even use different approaches so much as they aim for a different result--one that balances process and results and extends the definition of quality beyond the product itself to include cost and convenience to the customer as well. Ten methods of the Japanese kaizen culture of work are presented with applications and contrasts to American dentistry. PMID:9929991

  9. Transcriptional dynamics of the developing sweet cherry (Prunus avium L.) fruit: sequencing, annotation and expression profiling of exocarp-associated genes

    PubMed Central

    Alkio, Merianne; Jonas, Uwe; Declercq, Myriam; Van Nocker, Steven; Knoche, Moritz

    2014-01-01

    The exocarp, or skin, of fleshy fruit is a specialized tissue that protects the fruit, attracts seed dispersing fruit eaters, and has large economical relevance for fruit quality. Development of the exocarp involves regulated activities of many genes. This research analyzed global gene expression in the exocarp of developing sweet cherry (Prunus avium L., ‘Regina’), a fruit crop species with little public genomic resources. A catalog of transcript models (contigs) representing expressed genes was constructed from de novo assembled short complementary DNA (cDNA) sequences generated from developing fruit between flowering and maturity at 14 time points. Expression levels in each sample were estimated for 34 695 contigs from numbers of reads mapping to each contig. Contigs were annotated functionally based on BLAST, gene ontology and InterProScan analyses. Coregulated genes were detected using partitional clustering of expression patterns. The results are discussed with emphasis on genes putatively involved in cuticle deposition, cell wall metabolism and sugar transport. The high temporal resolution of the expression patterns presented here reveals finely tuned developmental specialization of individual members of gene families. Moreover, the de novo assembled sweet cherry fruit transcriptome with 7760 full-length protein coding sequences and over 20 000 other, annotated cDNA sequences together with their developmental expression patterns is expected to accelerate molecular research on this important tree fruit crop. PMID:26504533

  10. Transcriptional dynamics of the developing sweet cherry (Prunus avium L.) fruit: sequencing, annotation and expression profiling of exocarp-associated genes.

    PubMed

    Alkio, Merianne; Jonas, Uwe; Declercq, Myriam; Van Nocker, Steven; Knoche, Moritz

    2014-01-01

    The exocarp, or skin, of fleshy fruit is a specialized tissue that protects the fruit, attracts seed dispersing fruit eaters, and has large economical relevance for fruit quality. Development of the exocarp involves regulated activities of many genes. This research analyzed global gene expression in the exocarp of developing sweet cherry (Prunus avium L., 'Regina'), a fruit crop species with little public genomic resources. A catalog of transcript models (contigs) representing expressed genes was constructed from de novo assembled short complementary DNA (cDNA) sequences generated from developing fruit between flowering and maturity at 14 time points. Expression levels in each sample were estimated for 34 695 contigs from numbers of reads mapping to each contig. Contigs were annotated functionally based on BLAST, gene ontology and InterProScan analyses. Coregulated genes were detected using partitional clustering of expression patterns. The results are discussed with emphasis on genes putatively involved in cuticle deposition, cell wall metabolism and sugar transport. The high temporal resolution of the expression patterns presented here reveals finely tuned developmental specialization of individual members of gene families. Moreover, the de novo assembled sweet cherry fruit transcriptome with 7760 full-length protein coding sequences and over 20 000 other, annotated cDNA sequences together with their developmental expression patterns is expected to accelerate molecular research on this important tree fruit crop. PMID:26504533

  11. 33 CFR 334.430 - Neuse River and tributaries at Marine Corps Air Station Cherry Point, North Carolina; restricted...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Marine Corps Air Station Cherry Point, North Carolina; restricted area and danger zone. 334.430 Section... Air Station Cherry Point, North Carolina; restricted area and danger zone. (a) The restricted area... Station, Cherry Point, North Carolina, extending from the mouth of Hancock Creek to a point...

  12. 33 CFR 334.430 - Neuse River and tributaries at Marine Corps Air Station Cherry Point, North Carolina; restricted...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Marine Corps Air Station Cherry Point, North Carolina; restricted area and danger zone. 334.430 Section... Air Station Cherry Point, North Carolina; restricted area and danger zone. (a) The restricted area... Station, Cherry Point, North Carolina, extending from the mouth of Hancock Creek to a point...

  13. 33 CFR 334.430 - Neuse River and tributaries at Marine Corps Air Station Cherry Point, North Carolina; restricted...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Marine Corps Air Station Cherry Point, North Carolina; restricted area and danger zone. 334.430 Section... Air Station Cherry Point, North Carolina; restricted area and danger zone. (a) The restricted area... Station, Cherry Point, North Carolina, extending from the mouth of Hancock Creek to a point...

  14. 33 CFR 334.430 - Neuse River and tributaries at Marine Corps Air Station Cherry Point, North Carolina; restricted...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Marine Corps Air Station Cherry Point, North Carolina; restricted area and danger zone. 334.430 Section... Air Station Cherry Point, North Carolina; restricted area and danger zone. (a) The restricted area... Station, Cherry Point, North Carolina, extending from the mouth of Hancock Creek to a point...

  15. One dimensional Linescan x-ray detection of pits in fresh cherries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The presence of pits in processed cherries is a concern for both processors and consumers, in many cases causing injury and potential lawsuits. While machines used for pitting cherries are extremely efficient, if one or more plungers in a pitting head become misaligned, a large number of pits may p...

  16. Assessing the potential for establishment of western cherry fruit fly using ecological niche modeling

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sweet cherries, Prunus avium (L.) L., grown in the western United States are exported to many countries around the world. Some of these countries have enforced strict quarantine rules and trade restrictions due to concerns about the potential establishment and subsequent spread of western cherry fru...

  17. 33 CFR 208.82 - Hetch Hetchy, Cherry Valley, and Don Pedro Dams and Reservoirs.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Hetch Hetchy, Cherry Valley, and Don Pedro Dams and Reservoirs. 208.82 Section 208.82 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE FLOOD CONTROL REGULATIONS § 208.82 Hetch Hetchy, Cherry Valley, and Don Pedro Dams and...

  18. 33 CFR 208.82 - Hetch Hetchy, Cherry Valley, and Don Pedro Dams and Reservoirs.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Hetch Hetchy, Cherry Valley, and Don Pedro Dams and Reservoirs. 208.82 Section 208.82 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE FLOOD CONTROL REGULATIONS § 208.82 Hetch Hetchy, Cherry Valley, and Don Pedro Dams and...

  19. 75 FR 77564 - Tart Cherries Grown in the States of Michigan, et al.;

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-13

    ... Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, et al.; Final Free and Restricted Percentages for the 2010-2011 Crop Year for Tart Cherries AGENCY: Agricultural... referred to as the ``order.'' The order is effective under the Agricultural Marketing Agreement Act of...

  20. MILKWEED YELLOWS-RELATED PHYTOPLASMA IN BLUEBERRY AND A PREVIOUSLY UNDESCRIBED PHYTOPLASMA IN CHERRY

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two phytoplasma lineages were identified in diseased plants of sour cherry (Cerasus vulgaris Mill. Syn. Prunus cerasus) and European blueberry (Vaccinium myrtillus L.). On the basis of RFLP and nucleotide sequence analyses of 16S rDNA, the cherry little leaf (ChLL) phytoplasma was classified in gro...

  1. Sweet bing cherries lower circulating concentrations of markers for chronic inflammatory diseases in healthy humans

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A limited number of studies have demonstrated that some modulators of inflammation can be altered by the consumption of sweet cherries. We have taken a proteomics approach to determine the effect of dietary cherries on targeted gene expression. The purpose was then to determine changes in the concen...

  2. 7 CFR 52.812 - Score sheet for frozen red tart pitted cherries.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 2 2013-01-01 2013-01-01 false Score sheet for frozen red tart pitted cherries. 52.812 Section 52.812 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING... for Grades of Frozen Red Tart Pitted Cherries Score Sheet § 52.812 Score sheet for frozen red...

  3. 7 CFR 52.812 - Score sheet for frozen red tart pitted cherries.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 2 2014-01-01 2014-01-01 false Score sheet for frozen red tart pitted cherries. 52.812 Section 52.812 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING... for Grades of Frozen Red Tart Pitted Cherries Score Sheet § 52.812 Score sheet for frozen red...

  4. Astronauts Joseph Allen rides cherry picker over stowage area/work station

    NASA Technical Reports Server (NTRS)

    1984-01-01

    Astronaut Joseph P. Allen rides a cherry picker over to a stowage area/work station to wrap up extravehicular activity (EVA) duties above Earth. The cherry picker is a union of the mobile foot restraint and the remote manipulator system (RMS), controlled from inside Discovery's cabin. The Westar VI/PAM-D satellite is pictured secured in Discovery's cargo bay.

  5. Real-time methods for non-destructive detection of pits in fresh cherries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The presence of pits in processed cherries is a concern for both processors and consumers, in many cases causing injury and potential lawsuits. While machines used for pitting cherries are extremely efficient, if one or more plungers in a pitting head become misaligned, a large number of pits may p...

  6. Reduction of optimal thermal range in aging Western Cherry Fruit Flies(Rhagoletis indifferens Curan)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The western cherry fruit fly is an economically important pest of sweet cherries in the western United States. The potential of this pest to establish and spread in areas in which it is not currently present has been the focus of recent research. Most published information on the thermal tolerance a...

  7. Sensing the Moisture Content of Dry Cherries - A Rapid and Nondestructive Method

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Impedance (Z), and phase angle (') of a parallel-plate capacitor with a single cherry fruit between the plates was measured using a CI meter (Chari’s Impedance meter), at 1 and 9 MHz . Capacitance C, was derived from Z and ', and using the C, ', and Z values of a set of cherries whose moisture cont...

  8. Development of a systems approach for US cherries exported to Japan

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In order to develop a systems approach for codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), that will allow export of Pacific Northwest (Washington and Oregon) and California cherries to Japan, cherries were shown to be poor hosts for codling moth. Codling moth pheromone traps were pla...

  9. Bumblebee preference for symmetrical flowers.

    PubMed

    Møller, A P

    1995-03-14

    Fluctuating asymmetry, which represents small random deviations from otherwise bilateral symmetry, is a measure of the phenotypic quality of individuals indicating the ability of controlled development under given environmental and genetic conditions. I tested whether floral symmetry reliably reflects phenotypic quality measured in terms of pollinator rewards and whether pollinators respond to floral symmetry in a series of observations and experiments on Epilobium angustifolium (Onagraceae). Lower petal asymmetry was negatively related to mean lower petal length, whereas asymmetry in leaf width was positively related to mean leaf width. Flowers visited by bumblebees were larger and more symmetrical than the nearest neighboring flower. This relationship between pollinator preference for large and symmetrical flowers was demonstrated to be causal in experiments in which the lower petals were manipulated symmetrically or asymmetrically. Nectar production was larger in symmetrical flowers, and this may explain the bumblebee preference for flower symmetry. Floral symmetry therefore reliably reflects nectar production and hence enhances pollen transport. Extensive embryo abortion has been documented in E. angustifolium and other outcrossing plant species. Floral fluctuating asymmetry, which reflects general developmental homeostasis, may explain such developmental selection in these plants. PMID:11607519

  10. Acute kidney injury from cherry concentrate in a patient with CKD.

    PubMed

    Luciano, Randy L

    2014-03-01

    Nutraceuticals are supplements and medical foods that offer numerous health benefits. However, these substances may have adverse effects on multiple organ systems, leading to significant morbidity. I present a patient with chronic kidney disease who experienced hemodynamically mediated acute kidney injury and hyperkalemia after daily consumption of cherry concentrate. The method of injury was most likely cyclooxygenase inhibition by the compounds in cherries that mimic the mechanism of action of nonsteroidal anti-inflammatory medications. Ceasing cherry concentrate consumption led to improvements in both the patient's hyperkalemia and kidney injury. Physicians should be aware of the potentially harmful side effects of cherry concentrate and approach the use of cherry extract or concentrate with caution in patients with underlying kidney disease. PMID:24290246

  11. Population structure and genetic bottleneck in sweet cherry estimated with SSRs and the gametophytic self-incompatibility locus

    PubMed Central

    2010-01-01

    Background Domestication and breeding involve the selection of particular phenotypes, limiting the genomic diversity of the population and creating a bottleneck. These effects can be precisely estimated when the location of domestication is established. Few analyses have focused on understanding the genetic consequences of domestication and breeding in fruit trees. In this study, we aimed to analyse genetic structure and changes in the diversity in sweet cherry Prunus avium L. Results Three subgroups were detected in sweet cherry, with one group of landraces genetically very close to the analysed wild cherry population. A limited number of SSR markers displayed deviations from the frequencies expected under neutrality. After the removal of these markers from the analysis, a very limited bottleneck was detected between wild cherries and sweet cherry landraces, with a much more pronounced bottleneck between sweet cherry landraces and modern sweet cherry varieties. The loss of diversity between wild cherries and sweet cherry landraces at the S-locus was more significant than that for microsatellites. Particularly high levels of differentiation were observed for some S-alleles. Conclusions Several domestication events may have happened in sweet cherry or/and intense gene flow from local wild cherry was probably maintained along the evolutionary history of the species. A marked bottleneck due to breeding was detected, with all markers, in the modern sweet cherry gene pool. The microsatellites did not detect the bottleneck due to domestication in the analysed sample. The vegetative propagation specific to some fruit trees may account for the differences in diversity observed at the S-locus. Our study provides insights into domestication events of cherry, however, requires confirmation on a larger sampling scheme for both sweet cherry landraces and wild cherry. PMID:20727153

  12. Synchronous Pulsed Flowering: Analysis of the Flowering Phenology in Juncus (Juncaceae)

    PubMed Central

    Michalski, Stefan G.; Durka, Walter

    2007-01-01

    Background and Aims The timing of flowering within and among individuals is of fundamental biological importance because of its influence on total seed production and, ultimately, fitness. Traditional descriptive parameters of flowering phenology focus on onset and duration of flowering and on synchrony among individuals. These parameters do not adequately account for variability in flowering across the flowering duration at individual and population level. This study aims to analyse the flowering phenology of wind-pollinated Juncus species that has been described as temporally highly variable (‘pulsed flowering’). Additionally, an attempt is made to identify proximate environmental factors that may cue the flowering, and ultimate causes for the flowering patterns are discussed. Methods Flowering phenology was examined in populations of nine Juncus species by estimating flowering synchrony and by using the coefficient of variation (CV) to describe the temporal variation in flowering on individual and population levels. Phenologies were compared with null models to test which patterns deviate from random flowering. All parameters assessed were compared with each other and the performance of the parameters in response to randomization and varying synchrony was evaluated using a model population. Flowering patterns were correlated with temperature and humidity. Key Results Most flowering patterns of Juncus were best described as synchronous pulsed flowering, characterized as population-wide concerted flowering events separated by days with no or few open flowers. Flowering synchrony and variability differed from a random pattern in most cases. CV values in combination with a measure of synchrony differentiated among flowering patterns found. Synchrony varied among species and was independent from variability in flowering. Neither temperature nor humidity could be determined as potential cues for the flowering pulses. Conclusions The results indicate that selection

  13. Models for the beginning of sour cherry blossom

    NASA Astrophysics Data System (ADS)

    Matzneller, Philipp; Blümel, Klaus; Chmielewski, Frank-M.

    2013-03-01

    Seven different model approaches to calculate the onset of sour cherry blossom for the main growing regions in Rhineland-Palatinate (Germany) were compared. Three of the approaches were pure forcing models (M1, M2, M2DL) and the remaining four models were combined sequential chilling-forcing (CF) models. Model M1 was the commonly used growing degree day (GDD) model in which the starting date of temperature accumulation (t 1), the base temperature (T BF) and the forcing requirement F* were optimized on the basis of observed data. Because of a relatively late optimal starting date (t 1 = 1 March), the model can be applied only to calculate the onset of cherry blossom for present climate conditions. In order to develop forcing models that could possibly be used to estimate possible shifts in the timing of cherry blossom due to climate change, the starting date t 1 of the models was intentionally set to 1 January (M2, M2DL). Unfortunately, model M2 failed in both the optimization and validation period. The introduction of a daylength term (DL) in model M2DL improved model performance. In order to project possible shifts in the timing of plant phenological events, combined CF-models are preferred over pure GDD-models. For this reason four CF-models were developed with (M3DL, M4DL) and without (M3, M4) consideration of daylength in the GDD-approach. The chilling requirement was calculated using chilling hours (M3, M3DL) and chill portions (M4, M4DL). Both models without daylength estimated implausible model parameters and failed model validation. However, models M3DL and M4DL showed meaningful model parameter estimations and the error between modelled and observed data was markedly reduced. Moreover, the models optimized and validated (internal validation) for one sour cherry growing region in Germany, were applied successfully to calculate the beginning of the blossom period in other regions in Europe and even at one station in North America (external validation).

  14. South wing roof from the northeast, taken from a cherry ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South wing roof from the northeast, taken from a cherry picker. This view is particularly good in capturing the monitor and the dormers that provided light and ventilation for the third-floor rooms. At center are two of the original stone chimneys and at right center is the elevator's mechanical penthouse. The severity of the 2003 fire damage is evident at the bottom of the photograph. capturing the central pavilion. - U. S. Naval Asylum, Biddle Hall, Gray's Ferry Avenue, Philadelphia, Philadelphia County, PA

  15. Models for the beginning of sour cherry blossom

    NASA Astrophysics Data System (ADS)

    Matzneller, Philipp; Blümel, Klaus; Chmielewski, Frank-M.

    2014-07-01

    Seven different model approaches to calculate the onset of sour cherry blossom for the main growing regions in Rhineland-Palatinate (Germany) were compared. Three of the approaches were pure forcing models (M1, M2, M2DL) and the remaining four models were combined sequential chilling-forcing (CF) models. Model M1 was the commonly used growing degree day (GDD) model in which the starting date of temperature accumulation ( t 1), the base temperature ( T BF) and the forcing requirement F* were optimized on the basis of observed data. Because of a relatively late optimal starting date ( t 1 = 1 March), the model can be applied only to calculate the onset of cherry blossom for present climate conditions. In order to develop forcing models that could possibly be used to estimate possible shifts in the timing of cherry blossom due to climate change, the starting date t 1 of the models was intentionally set to 1 January (M2, M2DL). Unfortunately, model M2 failed in both the optimization and validation period. The introduction of a daylength term (DL) in model M2DL improved model performance. In order to project possible shifts in the timing of plant phenological events, combined CF-models are preferred over pure GDD-models. For this reason four CF-models were developed with (M3DL, M4DL) and without (M3, M4) consideration of daylength in the GDD-approach. The chilling requirement was calculated using chilling hours (M3, M3DL) and chill portions (M4, M4DL). Both models without daylength estimated implausible model parameters and failed model validation. However, models M3DL and M4DL showed meaningful model parameter estimations and the error between modelled and observed data was markedly reduced. Moreover, the models optimized and validated (internal validation) for one sour cherry growing region in Germany, were applied successfully to calculate the beginning of the blossom period in other regions in Europe and even at one station in North America (external validation).

  16. A Phase Transition for Circle Maps and Cherry Flows

    NASA Astrophysics Data System (ADS)

    Palmisano, Liviana

    2013-07-01

    We study C 2 weakly order preserving circle maps with a flat interval. The main result of the paper is about a sharp transition from degenerate geometry to bounded geometry depending on the degree of the singularities at the boundary of the flat interval. We prove that the non-wandering set has zero Hausdorff dimension in the case of degenerate geometry and it has Hausdorff dimension strictly greater than zero in the case of bounded geometry. Our results about circle maps allow to establish a sharp phase transition in the dynamics of Cherry flows.

  17. Comparison of postmenopausal endogenous sex hormones among Japanese, Japanese Brazilians, and non-Japanese Brazilians

    PubMed Central

    2011-01-01

    Background Differences in sex hormone levels among populations might contribute to the variation in breast cancer incidence across countries. Previous studies have shown higher breast cancer incidence and mortality among Japanese Brazilians than among Japanese. To clarify the difference in hormone levels among populations, we compared postmenopausal endogenous sex hormone levels among Japanese living in Japan, Japanese Brazilians living in the state of São Paulo, and non-Japanese Brazilians living in the state of São Paulo. Methods A cross-sectional study was conducted using a control group of case-control studies in Nagano, Japan, and São Paulo, Brazil. Participants were postmenopausal women older than 55 years of age who provided blood samples. We measured estradiol, estrone, androstenedione, dehydroepiandrosterone sulfate (DHEAS), testosterone and free testosterone by radioimmunoassay; bioavailable estradiol by the ammonium sulfate precipitation method; and sex hormone-binding globulin (SHBG) by immunoradiometric assay. A total of 363 women were included for the present analyses, comprising 185 Japanese, 44 Japanese Brazilians and 134 non-Japanese Brazilians. Results Japanese Brazilians had significantly higher levels of estradiol, bioavailable estradiol, estrone, testosterone and free testosterone levels, and lower SHBG levels, than Japanese. Japanese Brazilians also had significantly higher levels of bioavailable estradiol, estrone and DHEAS and lower levels of SHBG and androstenedione than non-Japanese Brazilians. Levels of estradiol, testosterone and free testosterone, however, did not differ between Japanese Brazilians and non-Japanese Brazilians. These differences were observed even after adjustment for known breast cancer risk factors. We also found an increase in estrogen and androgen levels with increasing body mass index, but no association for most of the other known risk factors. Conclusions We found higher levels of estrogens and androgens in

  18. New Frontiers for Japanese Youth

    ERIC Educational Resources Information Center

    Tucker, Frank H.

    1974-01-01

    Japanese literature, television, movies, and school texts from 1935 to 1955 are analyzed for their influence and contribution to Japanese youths' pioneering spirit and frontiermindedness. "Asian Affairs" is published by the American-Asian Educational Exchange, New York. (DE)

  19. Japanese Encephalitis: Frequently Asked Questions

    MedlinePlus

    ... of Page How long does the Japanese encephalitis vaccination last? The duration of protection is unknown. For ... What are the side effects of Japanese encephalitis vaccination? Pain and tenderness are the most commonly reported ...

  20. FLOBOTS: ROBOTIC FLOWERS FOR BEE BEHAVIOUR EXPERIMENTS

    PubMed Central

    Essenberg, Carla J.

    2015-01-01

    Studies of pollinator foraging behaviour often require artificial flowers that can refill themselves, allowing pollinators to forage for long periods of time under experimental conditions. Here I describe a design for inexpensive flowers that can refill themselves upon demand and that are easy enough to set up and clean that they can be used in arrays of 30 or more flowers. I also summarize of a variety of artificial flower designs developed by other researchers. PMID:25722755

  1. Spring Flowers: Harvest of a Sensitive Eye

    ERIC Educational Resources Information Center

    Clark, Eloise; Levin, Ted

    1978-01-01

    Defining and describing a number of spring flowers, this article includes illustrations and explanations that demonstrate "art and science are born of the same parents". The flowers discussed are skunk cabbage, bellwort, spring beauty, jack-in-the-pulpit, Solomon's seal, wild geranium, showy orchids, moccasin flower, bluets, apple, and Indian…

  2. Generation and characterization of Osterix-Cherry reporter mice.

    PubMed

    Strecker, Sara; Fu, Yu; Liu, Yaling; Maye, Peter

    2013-04-01

    Osterix is a zinc finger containing transcription factor, which functions as a key regulator of osteoblast differentiation. To better understand the temporal and spatial expression of Osterix during embryonic development and in the adult skeleton, we generated Osterix-Cherry reporter mice. Bacterial recombination techniques were employed to engineer a transgenic construct, which consisted of a ∼39 kb DNA fragment encompassing the Osterix/Sp7 gene, but excluding adjacent gene sequences. Osterix reporter expression was characterized at embryonic, neonatal, and adult ages both by itself and in the context of a cross with Bone Sialoprotein (BSP)-Topaz reporter mice. Relative to Osterix, BSP is a more mature marker of osteoblast differentiation. In agreement with osteoblast lineage maturation, Osterix reporter expression preceded BSP reporter expression during embryonic development and spatially appeared in a much broader cell population. Strong Osterix reporter expression was observed in mature osteoblasts and osteocytes. However, weaker Osterix-Cherry positive cells were also observed in the bone marrow, possibly identifying an early osteoprogenitor cell population. Evaluation of Osterix reporter expression in male femur tissue sections from 10 days to 12 weeks of age revealed persistent expression in cells of the osteoblast lineage and a surprising increase in maturing chondrocytes of the growth plate. Also, Osterix reporter expression was transiently detected in the kidney after birth. PMID:23180553

  3. Pyrethrum flowers and pyrethroid insecticides.

    PubMed Central

    Casida, J E

    1980-01-01

    The natural pyrethrins from the daisy-like flower, Tanacetum or Chrysanthemum cinerariifolium, are nonpersistent insecticides of low toxicity to mammals. Synthetic analogs or pyrethroids, evolved from the natural compounds by successive isosteric modifications, are more potent and stable and are the newest important class of crop protection chemicals. They retain many of the favorable properties of the pyrethrins. PMID:6993201

  4. Flowers and Landscape by Serendipity.

    ERIC Educational Resources Information Center

    Pippin, Sandi

    2003-01-01

    Describes an art lesson in which students sketch drawings of flowers and use watercolor paper and other materials to paint a landscape. Explains that the students also learn about impressionism in this lesson. Discusses how the students prepare the paper and create their artwork. (CMK)

  5. Cultural Competence in Business Japanese.

    ERIC Educational Resources Information Center

    Koike, Shohei

    Cultural competence in business Japanese requires more than superficial knowledge of business etiquette. One must truly understand why Japanese people think and act differently from their American counterparts. For example, instruction in the use of Japanese taxis must be accompanied by instruction in the concept and implications of seating order…

  6. Melatonin and its potential biological functions in the fruits of sweet cherry.

    PubMed

    Zhao, Yu; Tan, Dun-Xian; Lei, Qiong; Chen, Hao; Wang, Lin; Li, Qing-tian; Gao, Yinan; Kong, Jin

    2013-08-01

    Melatonin is a well-known molecule which possesses many beneficial effects on human health. Many agriculture products provide natural melatonin in the diet. Cherry is one such fruit as they are rich in melatonin. In order to understand the biological roles of melatonin in cherry fruit, melatonin synthesis and its changes over 24 hr period were systematically monitored both during their development and in the ripe cherries in two cultivars, 'Hongdeng' (Prunus avium L. cv. Hongdeng) and 'Rainier' (Prunus avium L. cv. Rainier). It was found that both darkness and oxidative stress induced melatonin synthesis, which led to dual melatonin synthetic peaks during a 24 hr period. The high levels of malondialdehyde induced by high temperature and high intensity light exposure were directly related to up-regulated melatonin production. A primary function of melatonin in cherry fruits is speculated to be as an antioxidant to protect the cherry from the oxidative stress. Importantly, plant tryptophan decaboxylase gene (PaTDC) was identified in cherry fruits. Our data shows that PaTDC expression is positively related to the melatonin production in the cherry. This provides additional information to suggest that tryptophan decaboxylase is a rate-limiting enzyme of melatonin synthesis in plants. PMID:23480341

  7. Hydrologic data for the drainage basins of Chatfield and Cherry Creek Lakes, Denver metropolitan area, Colorado

    USGS Publications Warehouse

    Gibbs, J.W.; Arnold, L.M.; Reed, R.L.

    1983-01-01

    Chatfield and Cherry Creek Lakes are flood control lakes constructed by the U.S. Army Corps of Engineers and leased to the Colorado Division of Parks and Recreation. Both lakes are in the Denver metropolitan area and provide a variety of recreational activities, including boating, camping, fishing, picnicking, and swimming. The projected increase of urban development in the drainage basins of Chatfield and Cherry Creek lakes could increase the constituent loads delivered to the lakes. Due to the eutrophic condition of Cherry Creek Lake and the potential eutrophic condition of Chatfield Lake, increased constituent loads could affect the suitability of the lakes for recreation. A monitoring program was started to determine the constituent loads of the drainage basins to both lakes. A network of monitoring stations was established to collect ambient water quality samples, storm runoff water quality samples, precipitation, and stream discharge. In the Cherry Creek basin 12 observation wells were established in the alluvium upgradient from Cherry Creek lake. Water levels and water quality data were collected to determine the quantity and quality of groundwater entering Cherry Creek lake. Data were collected from January through December 1982. The data may be used to evaluate the present and projected impact of urbanization in the drainage basins and the effect of increased constituent loads delivered to Chatfield and Cherry Creek lakes. (Author 's abstract)

  8. Tracking Cholesterol/Sphingomyelin-Rich Membrane Domains with the Ostreolysin A-mCherry Protein

    PubMed Central

    Skočaj, Matej; Resnik, Nataša; Grundner, Maja; Ota, Katja; Rojko, Nejc; Hodnik, Vesna; Anderluh, Gregor; Sobota, Andrzej; Maček, Peter; Veranič, Peter; Sepčić, Kristina

    2014-01-01

    Ostreolysin A (OlyA) is an ∼15-kDa protein that has been shown to bind selectively to membranes rich in cholesterol and sphingomyelin. In this study, we investigated whether OlyA fluorescently tagged at the C-terminal with mCherry (OlyA-mCherry) labels cholesterol/sphingomyelin domains in artificial membrane systems and in membranes of Madin-Darby canine kidney (MDCK) epithelial cells. OlyA-mCherry showed similar lipid binding characteristics to non-tagged OlyA. OlyA-mCherry also stained cholesterol/sphingomyelin domains in the plasma membranes of both fixed and living MDCK cells, and in the living cells, this staining was abolished by pretreatment with either methyl-β-cyclodextrin or sphingomyelinase. Double labelling of MDCK cells with OlyA-mCherry and the sphingomyelin-specific markers equinatoxin II–Alexa488 and GST-lysenin, the cholera toxin B subunit as a probe that binds to the ganglioside GM1, or the cholesterol-specific D4 domain of perfringolysin O fused with EGFP, showed different patterns of binding and distribution of OlyA-mCherry in comparison with these other proteins. Furthermore, we show that OlyA-mCherry is internalised in living MDCK cells, and within 90 min it reaches the juxtanuclear region via caveolin-1–positive structures. No binding to membranes could be seen when OlyA-mCherry was expressed in MDCK cells. Altogether, these data clearly indicate that OlyA-mCherry is a promising tool for labelling a distinct pool of cholesterol/sphingomyelin membrane domains in living and fixed cells, and for following these domains when they are apparently internalised by the cell. PMID:24664106

  9. Difference in flowering time can initiate speciation of nocturnally flowering species.

    PubMed

    Matsumoto, Tomotaka; Yasumoto, Akiko A; Nitta, Kozue; Hirota, Shun K; Yahara, Tetsukazu; Tachida, Hidenori

    2015-04-01

    Isolation mechanisms that prevent gene flow between populations prezygotically play important roles in achieving speciation. In flowering plants, the nighttime flowering system provides a mechanism for isolation from diurnally flowering species. Although this system has long been of interest in evolutionary biology, the evolutionary process leading to this system has yet to be elucidated because of the lack of good model species. However, the genetic mechanisms underlying the differences in flowering times and the traits that attract pollinators between a pair of diurnally and nocturnally flowering species have recently been identified in a few cases. This identification enables us to build a realistic model for theoretically studying the evolution of a nocturnally flowering species. In this study, based on previous experimental data, we assumed a model in which two loci control the flowering time and one locus determines a trait that attracts pollinators. Using this model, we evaluated the possibility of the evolution of a nocturnally flowering species from a diurnally flowering ancestor through simulations. We found that a newly emerging nighttime flowering flower exhibited a sufficiently high fitness, and the evolution of a nocturnally flowering species from a diurnally flowering species could be achieved when hybrid viability was intermediate to low, even in a completely sympatric situation. Our results suggest that the difference in flowering time can act as a magic trait that induces both natural selection and assortative mating and would play an important role in speciation between diurnally and nocturnally flowering species pairs. PMID:25665720

  10. Dispersal of Rhagoletis cerasi in Commercial Cherry Orchards: Efficacy of Soil Covering Nets for Cherry Fruit Fly Control

    PubMed Central

    Daniel, Claudia; Baker, Brian

    2013-01-01

    Demand for organic cherries offers producers a premium price to improve their commercial viability. Organic standards require that producers find alternatives to pesticides. Soil treatments to control the European cherry fruit fly Rhagoletis cerasi (L.) (Diptera: Tephrididae) appear to be an attractive option. However, soil treatments can only be effective if the migration of flies is low, because mature flies may migrate from near-by trees for oviposition. To examine the general potential of soil treatments and to understand the dispersal and flight behaviour of R. cerasi within orchards, experiments using netting to cover the soil were conducted in two orchards with different pest pressure during two years. The netting reduced flight activity by 77% and fruit infestation by 91%. The data showed that the flies have a dispersal of less than 5 m within orchards, which is very low. The low thresholds for tolerance for infested fruit in the fresh market creates a strong economic incentive for control, therefore, soil covering is a promising strategy for controlling R. cerasi in commercial orchards. PMID:26466801