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1

Kako natan?na je lahko transkripcija  

E-print Network

analysis. Ur. Deborah Schiffrin, Deborah Tannen, Heidi E. Hamilton. Oxford: Blackwell. 321–348. Michael Alexander Kirkwood HALLIDAY, 1998: An Introduction To Functional Grammar. 6th impression. London. 292–308. Klaus von HEUSINGER, 1999: Intonation... 2000. 6 Glej dalje zgled mh mh mh. — 163 — Kako natan?na je lahko transkripcija Tak je na primer Hallidayev pristop (Halliday 1998: 292–308) k pojmovanju intonacije oz. tonskega poteka, ki je predmet avtorjevih raziskav poleg ritma in tona. Za...

Ivi?, Mira Krajnc

2010-02-01

2

Role of Na+/H+ exchange by interferon-gamma in enhanced expression of JE and I-A beta genes  

SciTech Connect

The rapid transductional sequences initiated by interferon-gamma (IFN-gamma) on binding to its receptor regulate functional and genomic responses in many cells but are not well defined. Induction of macrophage activation is an example of such functional and genomic changes in response to IFN-gamma. Addition of IFN-gamma to murine macrophages, at activating concentrations, produced rapid (within 60 seconds) alkalinization of the cytosol and a concomitant, rapid influx of /sup 22/Na+. Amiloride inhibited the ion fluxes and the accumulation of specific messenger RNA for two genes induced by IFN-gamma (the early gene JE and the beta chain of the class II major histocompatibility complex gene I-A). The data indicate that IFN-gamma initiates rapid exchange of Na+ and H+ by means of the Na+/H+ antiporter and that these amiloride-sensitive ion fluxes are important to some of the genomic effects of IFN-gamma.

Prpic, V.; Yu, S.F.; Figueiredo, F.; Hollenbach, P.W.; Gawdi, G.; Herman, B.; Uhing, R.J.; Adams, D.O.

1989-04-28

3

Prefixov strom lohou je vytvoit prefixov strom z danho seznamu slov. Seznam slov pro postaven stromu je  

E-print Network

stromu je tvoen vsemi odkazy na webové stránce, jejíz URL je zadána jako vstup programu. Výsledkem je -jar PrefixTree.jar URL> URL>: URL webové stránky pro získání odkaz. Formát výstupu: Výstupem je implementaci: Pi zadání nevalidní URL program vypíse na standardní chybový výstup zprávu ,,URL is invalid

4

Wat ben je? BART JACOBS  

E-print Network

261 Wat ben je? BART JACOBS Hoogleraar computerbeveiliging; lid nationale Cyber Security Raad in ideeen-002.indd 261 03-09-13 15:39 #12;262 Bart Jacobs kel het attribuut `boven de 18' of je een heftige

Jacobs, Bart

5

Je bent minstens 18 jaar. Je verblijft wettig in Belgi.  

E-print Network

behaald bij de start van het voortraject (verplichte instaptest). · Je hebt minstens secundair onderwijs taalcoaching. · Studievaardigheden. · Studieloopbaanbegeleiding (kennismaking met het hoger onderwijs, hulp bij.debaets@hvngent.be 09/ 235 26 72 www.hvngent.be Oost-Vlaanderen educatie Voortraject hoger onderwijs voor anderstaligen

Gent, Universiteit

6

[Background of recent JE vaccine issues].  

PubMed

In Japan, more than 5,000 patients were reported in 1950, and there have been less than 10 cases annually since 1992. However, Japanese encephalitis virus caused of Japanese encephalitis (JE) are still existed highly and widely in the country, reported by National Institute of Infectious Diseases with serological examination among domestic pig population. JE immunization had been provided to children as category 1 routine immunization in Japan. However, the Ministry of Health, Labor and Welfare (HOHLW) decided not to recommend JE immunization to children as a routine immunization at May 2005. Major reason on this decision was that the Minter of MOHLW certified to pay loss of medical costs for the case of ADEM (acute disseminated encephalomyelopathy) after JE immunization, recognized as adverse events with JE vaccine, although MOHLW stated that the strict scientific evidence was unknown. MOHLW stated also that it is expected Vero cell derived JE vaccine should be replaced with the present mouse brain derived JE vaccine as the next generation, to be able to avoid theoretical possibility of neurological adverse events associated with JE vaccine. Small but increasing number of requests recently to be certified as health injuries on ADEM cases associated with JE immunization is also another reason for MOHLLW's decision. Further, fifth doses of JE vaccine given to children at 14-15 years old as a routine immunization was decided to be discontinued by MOHLW at July 2005, considering present epidemiological situation on JE and JE immunization status in Japan, although four doses has been recommended continuously as routine. The background details on JE vaccine issues decided by MOHLW in 2005 were reviewed on this paper. PMID:16557017

Okabe, Nobuhiko

2005-12-01

7

128. Credit JE. Outlet of tunnel on South Battle Creek ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

128. Credit JE. Outlet of tunnel on South Battle Creek Canal immediately above Junction with Cross Country Canal. (JE, v. 25 1910 p. 118). - Battle Creek Hydroelectric System, Battle Creek & Tributaries, Red Bluff, Tehama County, CA

8

EVALUATION OF UNCERTAINTY IN THE J&E MODEL  

EPA Science Inventory

This presentation presents an evaluation of uncertainty in the J&E model. The outline for the presentation is: general commentary on modeling; uncertainty analysis on J&E model; uncertainty reduction analysis; generic best/worst case determination; and correlation of soil and bu...

9

A Single Dose of Vero Cell-Derived Japanese Encephalitis (JE) Vaccine (Ixiaro) Effectively Boosts Immunity in Travelers Primed With Mouse Brain-Derived JE Vaccines  

PubMed Central

Background.?A significant part of the world population lives in areas with endemic Japanese encephalitis (JE). For travelers from nonendemic countries, Vero cell–derived vaccine (JE-VC; Ixiaro) has replaced traditional mouse brain–derived vaccines (JE-MB) associated with safety concerns. The 2 vaccines are derived from different viral strains: JE-VC from the SA14-14-2 strain and JE-MB from the Nakayama strain. No data exist regarding whether JE-VC can be used to boost immunity after a primary series of JE-MB; therefore, a primary series of JE-VC has been recommended to all travelers regardless of previous vaccination history. Methods.?One hundred twenty travelers were divided into 4 groups: Volunteers with no prior JE vaccination received primary immunization with (group 1) JE-MB or (group 2) JE-VC, and those primed with JE-MB received a single booster dose of (group 3) JE-MB or (group 4) JE-VC. Immune responses were tested before and 4–8 weeks after vaccination using plaque reduction neutralization test (PRNT) against both vaccine strains. Results.?In vaccine-naive travelers, the vaccination response rate for test strains Nakayama and SA14-14-2 was 100% and 87% after primary vaccination with JE-MB and 87% and 94% after JE-VC, respectively. Antibody levels depended on the target virus, with higher titers against homologous than heterologous PRNT50 target strain (P < .001). In travelers primed with JE-MB, vaccination response rates were 91% and 91%, and 98% and 95% after a booster dose of JE-MB or JE-VC, respectively. Subgroup analysis revealed that a higher proportion of primed (98%/95%) than nonprimed (39%/42%) volunteers responded to a single dose of JE-VC (P < .001). Conclusions.?A single dose of JE-VC effectively boosted immunity in JE-MB–primed travelers. Current recommendations should be reevaluated. Clinical Trials Registration.?NCT01386827. PMID:22696017

Erra, Elina O.; Askling, Helena Hervius; Rombo, Lars; Riutta, Jukka; Vene, Sirkka; Yoksan, Sutee; Lindquist, Lars; Pakkanen, Sari H.; Huhtamo, Eili; Vapalahti, Olli; Kantele, Anu

2012-01-01

10

Memory immune response and safety of a booster dose of Japanese encephalitis chimeric virus vaccine (JE-CV) in JE-CV-primed children  

PubMed Central

Japanese encephalitis chimeric virus vaccine (JE-CV) is a licensed vaccine indicated in a single dose administration for primary immunization. This controlled phase III comparative trial enrolled children aged 36–42 mo in the Philippines. 345 children who had received one dose of JE-CV in a study two years earlier, received a JE-CV booster dose. 105 JE-vaccine-naïve children in general good health were randomized to receive JE-CV (JE-vaccine naïve group; 46 children) or varicella vaccine (safety control group; 59 children). JE neutralizing antibody titers were assessed using PRNT50. Immunological memory was observed in children who had received the primary dose of JE-CV before. Seven days after the JE-CV booster dose administration, 96.2% and 66.8% of children were seroprotected and had seroconverted, respectively, and the geometric mean titer (GMT) was 231 1/dil. Twenty-eight days after the JE-CV booster dose seroprotection and seroconversion were achieved in 100% and 95.3% of children, respectively, and the GMT was 2,242 1/dil. In contrast, only 15.4% of JE-CV-vaccine naïve children who had not received any prior JE vaccine were seroprotected seven days after they received JE-CV. One year after receiving the JE-CV booster dose, 99.4% of children remained seroprotected. We conclude that JE-CV is effective and safe, both as a single dose and when administrated as a booster dose. A booster dose increases the peak GMT above the peak level reached after primary immunization and the antibody persistence is maintained at least one year after the JE-CV booster dose administration. Five year follow up is ongoing. PMID:23442823

Feroldi, Emmanuel; Capeding, Maria Rosario; Boaz, Mark; Gailhardou, Sophia; Meric, Claude; Bouckenooghe, Alain

2013-01-01

11

Je-S Guidance Notes for Applicants G22v4/ML/Je-SGuide February 2011 Page 1 of 23  

E-print Network

for Applicants applying for funding Je-S web page: https://je-s.rcuk.ac.uk/ #12;Je-S Guidance Notes Councils iii. Submitting of reports for ongoing grants made by one of the UK Research Councils. All funding Costing (FEC) methodology Arts and Humanities Research Council (AHRC) Biotechnology and Biological

12

Resonance and Capture of Jupiter Comets , J.E. Marsden  

E-print Network

Resonance and Capture of Jupiter Comets W.S. Koon , M.W. Lo , J.E. Marsden and S.D. Ross January, 2001 Abstract. A number of Jupiter family comets such as Oterma and Gehrels 3 make a rapid transition from heliocentric orbits outside the orbit of Jupiter to heliocentric orbits inside the orbit

Koon, Wang Sang

13

Magazine nationale veiligheid en crisisbeheersing november/december 20098 Pas op: in je blootje vat je kou!  

E-print Network

startschot voor een serie van TV-spotjes om mensen beter bewust te maken van de persoonlijke gegevens die, in blootje vat je kou!". Is hier sprake van hypocrisie? Good guys en bad guys Impliciet bij deze ontwikkelingen is een aantal vooronderstellingen, met name over wie good guys en bad guys zijn. De overheid

Jacobs, Bart

14

104. Credit JE. Detail view of AllisChalmers Francistype turbine housing ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

104. Credit JE. Detail view of Allis-Chalmers Francis-type turbine housing and generator. (JE, v. 27 1911 p. 418). - Battle Creek Hydroelectric System, Battle Creek & Tributaries, Red Bluff, Tehama County, CA

15

105. Credit JE. Detail view of AllisChalmers Francistype turbine housing ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

105. Credit JE. Detail view of Allis-Chalmers Francis-type turbine housing and generator. (JE, v. 27 1911 p. 418). - Battle Creek Hydroelectric System, Battle Creek & Tributaries, Red Bluff, Tehama County, CA

16

The Effect of Precipitation on the Transmission of Japanese Encephalitis (JE) Virus in Nature: A Complex Effect on Antibody-Positive Rate to JE Virus in Sentinel Pigs  

PubMed Central

Japanese encephalitis (JE) is one of the most important mosquito-borne viral diseases in Asia. Pigs are a natural host and the amplifier of JE virus. The sero-conversion rate to JE virus in sentinel pigs reflects the activity of JE virus in the region. We analyzed whether precipitation has any effect on the sero-conversion rate to JE virus in sentinel pigs. Linear regression analysis was performed to determine the correlations between the levels of precipitation and sero-conversion rates to JE virus, in the entire year and during summertime over the period of 32 years from 1969 to 2000. The levels of the annual and summertime precipitation demonstrated statistically significant positive correlations with sero-conversion rates for the whole of the country and for some regions in Japan. The levels of the summertime precipitation, on the other hand, demonstrated statistically significant inverse correlations with the sero-conversion rates in other regions. Further, the levels of precipitation during preceding 10-day periods from days 1–40 before blood collection showed inverse correlation with antibody-positive rates in some regions. The results indicate that the relationship between the annual and summertime precipitation, and the sero-conversion rate to JE virus is complex; both positive and inverse effects are demonstrated depending on the regions. PMID:23644830

Kurane, Ichiro; Shibasaki, Ken-ichi; Kotaki, Akira; Hijioka, Yasuaki; Takasaki, Tomohiko

2013-01-01

17

The effect of precipitation on the transmission of Japanese encephalitis (JE) virus in nature: a complex effect on antibody-positive rate to JE virus in sentinel pigs.  

PubMed

Japanese encephalitis (JE) is one of the most important mosquito-borne viral diseases in Asia. Pigs are a natural host and the amplifier of JE virus. The sero-conversion rate to JE virus in sentinel pigs reflects the activity of JE virus in the region. We analyzed whether precipitation has any effect on the sero-conversion rate to JE virus in sentinel pigs. Linear regression analysis was performed to determine the correlations between the levels of precipitation and sero-conversion rates to JE virus, in the entire year and during summertime over the period of 32 years from 1969 to 2000. The levels of the annual and summertime precipitation demonstrated statistically significant positive correlations with sero-conversion rates for the whole of the country and for some regions in Japan. The levels of the summertime precipitation, on the other hand, demonstrated statistically significant inverse correlations with the sero-conversion rates in other regions. Further, the levels of precipitation during preceding 10-day periods from days 1-40 before blood collection showed inverse correlation with antibody-positive rates in some regions. The results indicate that the relationship between the annual and summertime precipitation, and the sero-conversion rate to JE virus is complex; both positive and inverse effects are demonstrated depending on the regions. PMID:23644830

Kurane, Ichiro; Shibasaki, Ken-ichi; Kotaki, Akira; Hijioka, Yasuaki; Takasaki, Tomohiko

2013-05-01

18

Review: Barbara Kry?an?- Stanojevi?: Lice i nali?je jezi?ne globalizacije  

E-print Network

Petruši?, jezik predstavlja le za izboljšanje ekonomije potrebno sredstvo komunikacije, ki je uspešnejša, ?e se izvaja v enem samem jeziku in ta jezik je obi?ajno angleš?ina. Elitiziranje in postavljanje dolo?enega jezika pred druge vsekakor nima...

Kova?, Mija

2012-01-01

19

Nare?je Ziljske doline in splošnoslovenski pomik cirkumfleksa  

E-print Network

zaradi tega, ker ohranja arhaizme, ki jih v drugih slovenskih nare?jih ni ve?. Urban Jarnik, ki mu je bila ziljš?ina materni jezik, je navedel nekaj teh arhaizmov (1842, prim. tudi Pronk 2007a): – Ziljš?ina ima kondicionalni pomožnik b??n, b??š, b?? itd... dajalniku ednine ženskih samostalnikov. Knjižni jezik je tu posplošil kon?nici -u in -i. Navedeni arhaizmi so znani iz najbolj zahodnega dela Ziljske doline (prim. Gra- fenauer 1905, Logar 1968, 1981, Pronk 2009). Za ostale govore imamo manj po- datkov...

Pronk, Tijmen

2011-01-01

20

Slovenski terminološki sistemi na primeru pravne terminologije  

E-print Network

motivirajo- ?ega pomena omogo?a natan?nost in nedvoumnost motiviranega. 4 Pogodba o Ustavi za Evropo (PUE) Za pravni red ES (acquis communautaire) 1#4; velja na?elo primarnosti, kar pomeni, da »pravna pravila, sprejeta na ravni EU, prevladajo nad pravnimi... tudi PUE postala del pri- marne zakonodaje. Njena priprava je bila napovedana v Izjavi o prihodnosti Unije (Laeken, decem- ber 2001), ki je bila dodana Pogodbi iz Nice. 1#4; V skladu z Laekensko izjavo se je o prihodnosti EU odlo?alo na Konvenciji o...

Fajfar, Tanja

2007-01-01

21

[The medical theory of Lee Je-ma and its character].  

PubMed

Lee Je-ma 1837-1900) was a prominent scholar as well as an Korean physician. classified every people into four distinctive types: greater yang [tai yang] person, lesser yin [shao yin] person, greater yin [tai yin] person, lesser yin [shao yin] person. This theory would dictate proper treatment for each type in accordance with individual differences of physical and temperament features. Using these four types he created The Medical Science of Four Types. This article is intended to look into the connection between Lee Je-Ma's 'The Medical Science of Four Types' and 'The Modern' with organizing his ideas about the human body and the human being. Through The Modern, the theory of human being underwent a complete change. Human being in The Premodern, which was determined by sex, age and social status has been changed to the individual human being, which is featured by equality. Lee Je-Ma's medical theory of The Medical Science of Four Types would be analyzed as follow. His concept of human body is oriented toward observable objectivity. But on the other hand, it still remains transcendent status of medical science, which is subordinated by philosophy. According to Lee Je-Ma's theory of human being, human is an equal individual in a modern way of thinking, not as a part of hierarchical group. But on the other hand, it still remains incomplete from getting rid of morality aspect that includes virtue and vice in the concept of human body. The common factors in Lee Je-Ma's ideas about the human body and the human being is 'Dualism of mind and body that means all kinds of status and results depends on each individual. As is stated above, Lee Je-Ma's medical theory has many aspects of The Modern and it proves that Korean traditional medicine could be modernized by itself. PMID:17144191

Lee, Kyung-Lock

2005-12-01

22

Role of some factors on the breeding of JE vector Culex vishnui group.  

PubMed

Vector of JE, Culex vishnui group, prefer to breed in rice field. During the present study, roles of some factors like irrigation, manuring, pesticide application, rainfall, temperature and pH of the breeding water, depth of breeding water and height of paddy plants on breeding of Culex vishnui group larvae have been studied. It is evident from the study that besides striking role of the factors like manuring and pesticide application, other factors have also some role on the breeding of JE vectors at their breeding sites. PMID:16506548

Banerjee, A; Chandra, G

2004-12-01

23

Literarnozgodovinska in književnokriti?na refleksija madžarske književnosti po letu 1980 v slovenš?ini  

E-print Network

seznanjali že od za?etka 20. stol. naprej; drugi?, teh uvidov je manj kot bi glede na bližino obeh narodov, njuno povezanost in pomen same literature pri?akovali, in tretji?, porast »slovenskega« interesa je opazen v zadnjih letih. The article considers... nekoliko podrobneje razloži t. i. ljudsko gibanje, ki se je v tridesetih letih 20. stol. cepilo v ve? tokov, njihova dejavnost pa je variirala od poudarjanja socialnih tem, simpatiziranja s kasnejšo soci- alisti?no oblastjo, neposrednega nasprotovanja...

Štuhec, Miran

2009-01-01

24

Vierde programmeeropdracht: Othello In deze opdracht leer je werken met recursie, stapels, Makefiles en Qt designer. Lees voor  

E-print Network

Vierde programmeeropdracht: Othello In deze opdracht leer je werken met recursie, stapels dictaat. In deze opdracht moet je (1) het spel Othello programmeren op een m Ã? n bord, (2) het spel weergeven met behulp van Qt. Het "normale" spel Othello wordt gespeeld op een 8 bij 8 bord. Er wordt

Kosters, Walter

25

Teaching Linear Algebra with and to Computers D.J.Je rey and Robert M. Corless  

E-print Network

Teaching Linear Algebra with and to Computers D.J.Je#11;rey and Robert M. Corless Ontario Research Centre for Computer Algebra University of Western Ontario Abstract Three topics are discussed that relate to the teaching of linear algebra using computers (here the term computers includes calculators). The #12;rst

Jeffrey, David

26

Dear JE Class Of 2018, If you asked the Admission Office why Jonathan  

E-print Network

in the dining hall. Stop by the Master's Office and meet the loving JE staff. Play IMs any or every season roughly 7' x 9.5' of floor space, but expect variations of a foot in either direction. Built-in wardrobes obligated to adhere to the track you set down in high school. Take advantage of shopping period to find new

Johnson, Marcia K.

27

Je fais un don de ................................ euros (chque l'ordre de Fondation INSA de Lyon)  

E-print Network

Lyon) Je désire que ma contribution soit utilisée pour : Boursed'accueil sur le campus (valeurde2000) Bourse« Stages ouvriers » (valeurde1200) BoursedesFilières Internationales (valeurde1000) Bourse'INSA de Lyon. Cette communication a pour objectif la promotion des projets et valeurs auxquels l'INSA de

Stouls, Nicolas

28

Variable effect on peripheral blood leucocytes during JE virus infection of man.  

PubMed Central

The peripheral blood leucocytes of twenty-four cases of Japanese encephalitis (JE) were studied and the findings were compared with those in twenty-five normal health controls of matching age and sex. In the early phases of the disease marked neutrophil leucocytosis was seen which returned to almost normal levels by the fourth week. Lymphopenia was associated with diminished T lymphocytes but the number of B lymphocytes remained within the normal range. Though the number of T lymphocytes was reduced, their function of leucocyte migration inhibition in the presence of JE virus antigen was significantly higher. The phagocytic activity of the neutrophils, as shown by the uptake of neutral red dye, was diminished but the phagocytic activity of monocytes as shown by the uptake of neutral red dye, was diminished but the phagocytic activity of monocytes as shown by the uptake of neutral red dye or ingestion of latex particles remained unaffected. HI antibodies against JE virus were significantly higher in cases of encephalitis as compared with the control group. Thus, JE virus infection in man has a variable effect on different components of the peripheral blood leucocytes. PMID:231492

Chaturvedi, U C; Mathur, A; Tandon, P; Natu, S M; Rajvanshi, S; Tandon, H O

1979-01-01

29

Variable effect on peripheral blood leucocytes during JE virus infection of man.  

PubMed

The peripheral blood leucocytes of twenty-four cases of Japanese encephalitis (JE) were studied and the findings were compared with those in twenty-five normal health controls of matching age and sex. In the early phases of the disease marked neutrophil leucocytosis was seen which returned to almost normal levels by the fourth week. Lymphopenia was associated with diminished T lymphocytes but the number of B lymphocytes remained within the normal range. Though the number of T lymphocytes was reduced, their function of leucocyte migration inhibition in the presence of JE virus antigen was significantly higher. The phagocytic activity of the neutrophils, as shown by the uptake of neutral red dye, was diminished but the phagocytic activity of monocytes as shown by the uptake of neutral red dye, was diminished but the phagocytic activity of monocytes as shown by the uptake of neutral red dye or ingestion of latex particles remained unaffected. HI antibodies against JE virus were significantly higher in cases of encephalitis as compared with the control group. Thus, JE virus infection in man has a variable effect on different components of the peripheral blood leucocytes. PMID:231492

Chaturvedi, U C; Mathur, A; Tandon, P; Natu, S M; Rajvanshi, S; Tandon, H O

1979-12-01

30

A Search for Host Galaxies of 24 Gamma Ray Bursts J.-E. Ovaldsen1  

E-print Network

A Search for Host Galaxies of 24 Gamma Ray Bursts J.-E. Ovaldsen1 , A. O. Jaunsen1 , J. P. U. Fynbo.e.ovaldsen@astro.uio.no ABSTRACT We report the results from observations of 24 gamma ray burst (GRB) fields from 2005 and 2006 than previous (i.e. pre-Swift) studies. Subject headings: gamma rays: bursts -- cosmology: observations

Ovaldsen, Jan-Erik

31

Shopbot Economics Je rey O. Kephart (kephart@us.ibm.com)  

E-print Network

Shopbot Economics Je#11;rey O. Kephart (kephart@us.ibm.com) IBM Institute for Advanced Commerce IBM@cs.brown.edu) Department of Computer Science Brown University, Box 1910 Providence, RI 02912 USA Abstract. Shopbots is not to explain human economic behavior, but rather to design economic software agents and study their behavior

Greenwald, Amy

32

Procedures for Reducing the Size of Coverage-based Test Sets A. Je erson O utt  

E-print Network

Procedures for Reducing the Size of Coverage-based Test Sets A. Je erson O utt ISSE Department, 4A4@rstcorp.com Abstract This paper addresses the problem of reducing the size of test sets for regression testing and test output inspection. Since regression testing requires the execution of some, and in the worst case, all

Offutt, Jeff

33

An Integrated Automatic Test Data Generation System A. Je erson O utt  

E-print Network

An Integrated Automatic Test Data Generation System A. Je erson O utt Department of Computer Science Clemson University Clemson, SC 29634 January 21, 1996 Abstract The Godzilla automatic test data generator is an integrated collection of tools that implements a relatively new test data generation method

Offutt, Jeff

34

Thermoelectric power factor enhancement by ionized nanoparticle scattering Je-Hyeong Bahk,1,a)  

E-print Network

Thermoelectric power factor enhancement by ionized nanoparticle scattering Je-Hyeong Bahk,1,a theoretically that the thermoelectric power factor can be enhanced in degenerate semiconductors when embedded intact. We find that the thermoelectric power factor of In0.53Ga0.47As from 300 K to 800 K is enhanced

35

Entomological investigations into an epidemic of Japanese encephalitis (JE) in northern districts of West Bengal, India (2011-2012)  

PubMed Central

Background & objectives: Japanese encephalitis (JE) is one of the most important arboviral diseases of human beings with outbreaks in many parts of Southeast Asia including India. We present the entomological findings of an outbreak occurred in northern part of West Bengal during 2011-2012 with special emphasis on the role of JE vectors in different seasons. Methods: Adult mosquito collections were made with the help of mouth aspirators, aided by flash lights during day time resting inside human and animal habitations as indoor, and resting outside field grasses, bushes, underneath of culverts and bridges as outdoor, and in and around the pig enclosures and cattle sheds during dusk period in JE affected villages from Cooch Behar, Dakshin Dinajpur, Darjeeling and Jalpaiguri districts in North West Bengal. In all study villages, a long handled with enamel bowl dipper was used to obtain immature stages of mosquitoes from various breeding habitats. Results: A total of 19 different types of mosquito breeding habitats were examined for vectors of JE. From these habitats, 23.7 per cent were positive for breeding during the study period. Overall, nine different species were recorded through emergence, but none was positive for JE virus when subjected for detection of virus. Adult mosquitoes of more than 50 per cent of the potential JE vector species obtained through dusk and the rest through indoor and outdoor collections in all seasons. Altogether, 27 different species were recorded. Most of these were JE vectors. Interpretation & conclusions: Our results showed that in addition to Cx. vishnui subgroup, detection of JE virus antigen in Cx. quinquefasciatus indicated the possible maintenance of JE virus in nature through poor vector mosquitoes throughout the year. Since, all potential vector species reported elsewhere in India were also found in this region and fluctuated in density in different seasons, a proper integrated vector control programme needs to be implemented to control JE transmission. PMID:25027086

Mariappan, T.; Samuel, P. Philip; Thenmozhi, V.; Paramasivan, R.; Sharma, Puran Kumar; Biswas, Asit Kumar; Tyagi, B.K.

2014-01-01

36

Demander une bourse : 3 tapes ETAPE 1 : Je prsente une demande de bourse  

E-print Network

Demander une bourse : 3 étapes ETAPE 1 : Je présente une demande de bourse - Entre le 15 janvier et procède alors à l'instruction de mon dossier. ETAPE 2 : Réception d'un avis conditionnel de bourse : - Dès conditionnelle de bourse indiquant (au verso) les décisions d'attribution ou de refus pour chacun de mes voeux d

Paris-Sud 11, Université de

37

Effective field theory for pulsepropagation in nonlinearphotonic crystals J.E. Sipe', Navin Bhat', Philiu Chak' and Suresh Pereira'  

E-print Network

', Navin Bhat', Philiu Chak' and Suresh Pereira' 'Department of Physics, Universityof Toronto, Toronto, ON] Suresh Pereira and J.E. Sipe, "Hamiltonian formulationof the nonlinear coupled mode equations'' Phys. Rev

Sipe,J. E.

38

Živalska frazeologija v govoru vasi Dob pri Pliberku na avstrijskem Koroškem  

E-print Network

- met raziskave. V iivalsko frazeologijo uvrgtamo FE z iivalskimi poimenovanji v se- stavi in njihove izpeljanke. Z iivalmi tesno povezanih poimenovanj kot npr. rnleko Prim. KriiSnik 1987: 49-56. Prim. tudi Petermann 1988: 301-310. Petermann se v prvi..., EeS da je tvorba analogna pojmom fonem, morfem, tonem itd. Prim. tudi KriiSnik-KolSek 1988: 43-44. Gle- de na vpraSanje, kaj je pravi analogon pojmom fonem, morfem itd., ugotavlja, da je to ~seveda frazem [...I*. Prim. tudi Petermann 1988: 307...

Trampusch, Tatjana

1999-01-01

39

Differential regulation of the JE gene encoding the monocyte chemoattractant protein (MCP-1) in cervical carcinoma cells and derived hybrids.  

PubMed Central

Malignant human papillomavirus type 18 (HPV18)-positive cervical carcinoma cells can be reverted to a nonmalignant phenotype by generation of somatic cell hybrids with normal human fibroblasts. Although nontumorigenic hybrids, their tumorigenic segregants, and the parental HeLa cells have similar in vitro properties, inoculation only of nontumorigenic cells into nude mice results in a selective suppression of HPV18 transcription which precedes cessation of cellular growth. Our present study, aimed at understanding the differential regulation in vitro and in vivo, shows that the JE gene, encoding the monocyte chemoattractant protein (MCP-1), is expressed only in nontumorigenic hybrids. Although the gene, including its regulatory region, is intact, no JE (MCP-1) mRNA is detected in the tumorigenic segregants and in other malignant HPV-positive cervical carcinoma cell lines. Tests of several monocyte-derived cytokines showed that only tumor necrosis factor alpha strongly induces the JE (MCP-1) gene in nontumorigenic cells and that this is accompanied by a dose-dependent reduction of HPV transcription. The JE (MCP-1) up-regulation occurs within 2 h and does not require de novo protein synthesis. The response to tumor necrosis factor alpha seems to be mediated by an NF-kappa B-related mechanism, since the induction can be completely abrogated by pretreating the cells with an antioxidant such as pyrrolidine dithiocarbamate. Interestingly, cocultivation of nonmalignant hybrids with monocyte-enriched fractions from human peripheral blood also results in an induction of the JE (MCP-1) gene and a concomitant suppression of HPV18 transcription. Neither effect is observed in malignant cells. These data suggest that JE (MCP-1) may play a pivotal role in the intercellular communication by triggering an intracellular pathway which negatively interferes with viral transcription in HPV-positive nontumorigenic cells. Images PMID:8138998

Rosl, F; Lengert, M; Albrecht, J; Kleine, K; Zawatzky, R; Schraven, B; zur Hausen, H

1994-01-01

40

DIERGENEESKUNDE GETS SOCIAL -MAAK JE EIGEN TWITTER-ACCOUNT IN 5 STAPPEN Nee, Twitter gaat net alleen over mensen die vertellen dat ze gaan koffiedrinken!  

E-print Network

DIERGENEESKUNDE GETS SOCIAL - MAAK JE EIGEN TWITTER-ACCOUNT IN 5 STAPPEN Nee, Twitter gaat níet informatie over collega's en klanten! Op Twitter gaat het over delen en uitwisselen. Op dit openbare, online aan de slag met één daarvan, met Twitter. 1. GA NAAR WWW.TWITTER.COM Vul daar je gegevens in bij `sign

Utrecht, Universiteit

41

Detection of dusty plasma near the E-ring of Saturn J.-E. Wahlund a,, M. Andre a  

E-print Network

Detection of dusty plasma near the E-ring of Saturn J.-E. Wahlund a,�, M. Andre´ a , A Keywords: Saturn Magnetosphere E-ring Cassini Dust-plasma interaction Electrodynamic coupling Plasma that charged dust in the E-ring interacts collectively with the dense surrounding plasma disk of Saturn, i

Gurnett, Donald A.

42

Sleep epidemiology -a rapidly growing field Ferrie JE, Kumari M, Salo P, Singh-Manoux A, Kivimaki M  

E-print Network

Sleep epidemiology - a rapidly growing field Ferrie JE, Kumari M, Salo P, Singh-Manoux A, Kivimaki such that it anticipates periods of sleep and activity. Deviations from this circadian rhythm come with functional of sleep die after 32 days,3 and, with longer periods of sleep deprivation, this would also be the case

Boyer, Edmond

43

COSTA PROBLEM UNDER CHANNEL AMBIGUITY J.E. Vila-Forcen, S. Voloshynovskiy, O. Koval and T. Pun  

E-print Network

COSTA PROBLEM UNDER CHANNEL AMBIGUITY J.E. Vila-Forc´en, S. Voloshynovskiy, O. Koval and T. Pun University of Geneva, Department of Computer Science. 24 rue G´en´eral-Dufour, CH 1211, Geneva, Switzerland ABSTRACT In this paper, we address the analysis of the Costa setup un- der channel uncertainty. Since

Genève, Université de

44

,,Vzdlvn je vc, nez jen penos informac." Katedra didaktiky fyziky Matematicko-fyzikln fakulty Univerzity Karlovy v Praze  

E-print Network

�v� vzdl�v�n�m. Je tvrcem vyucovac� metody Peer Instruction. Abstract Education is more than just transfer of lectures is to take down as many notes as they can. Few students have the ability, motivation -- and most elusive -- aspect of education. I will show how shifting the focus in lectures from delivering

Cerveny, Vlastislav

45

J.E. Randall D.G. Fautin Fishes other than anemonefishes that associate with sea anemones  

E-print Network

NOTE J.E. Randall Ã? D.G. Fautin Fishes other than anemonefishes that associate with sea anemones Accepted: 26 February 2002 / Published online: 14 June 2002 Ã? Springer-Verlag 2002 Keywords Sea anemones Ã? Fishes Ã? Facultative association Introduction The obligatory relationship of sea anemones

Fautin, Daphne

46

Prediction of Turbulence-Generated Noise in Unheated Jets. Part 2; JeNo Users' Manual (Version 1.0)  

NASA Technical Reports Server (NTRS)

JeNo (Version 1.0) is a Fortran90 computer code that calculates the far-field sound spectral density produced by axisymmetric, unheated jets at a user specified observer location and frequency range. The user must provide a structured computational grid and a mean flow solution from a Reynolds-Averaged Navier Stokes (RANS) code as input. Turbulence kinetic energy and its dissipation rate from a k-epsilon or k-omega turbulence model must also be provided. JeNo is a research code, and as such, its development is ongoing. The goal is to create a code that is able to accurately compute far-field sound pressure levels for jets at all observer angles and all operating conditions. In order to achieve this goal, current theories must be combined with the best practices in numerical modeling, all of which must be validated by experiment. Since the acoustic predictions from JeNo are based on the mean flow solutions from a RANS code, quality predictions depend on accurate aerodynamic input.This is why acoustic source modeling, turbulence modeling, together with the development of advanced measurement systems are the leading areas of research in jet noise research at NASA Glenn Research Center.

Khavaran, Abbas; Wolter, John D.; Koch, L. Danielle

2009-01-01

47

DU LAIT GERBER OU BABCOK ? 605 Quand j'ai voulu vrifier mes tubes de Gerber, je n'ai pu trouver  

E-print Network

DU LAIT GERBER OU BABCOK ? 605 Quand j'ai voulu vérifier mes tubes de Gerber, je n'ai pu trouver d)EVELDE Professeur à l'Institut agronomique et à I'Universi.té de Gand. Dans ina première communication (1), j'ai concentration avec le sol. Dans cette communication, j'ai annoncé également que je projetais de mettre les sols

Paris-Sud XI, Université de

48

Immunogenicity, Genetic Stability, and Protective Efficacy of a Recombinant, Chimeric Yellow Fever-Japanese Encephalitis Virus (ChimeriVax-JE) as a Live, Attenuated Vaccine Candidate against Japanese Encephalitis  

Microsoft Academic Search

Yellow fever (YF) 17D vaccine virus, having a 60-year history of safe and effective use, is an ideal vector to deliver heterologous genes from other medically important flaviviruses. A chimeric YF\\/Japanese encephalitis (JE) virus (ChimeriVax-JE virus) was constructed by insertion of the premembrane and envelope (prME) genes of an attenuated human vaccine strain (SA14–14-2) of Japanese encephalitis (JE) virus between

F. Guirakhoo; Z.-X. Zhang; T. J. Chambers; S. Delagrave; J. Arroyo; A. D. T. Barrett; T. P. Monath

1999-01-01

49

Japanese encephalitis (JE). Part I: clinical profile of 1,282 adult acute cases of four epidemics.  

PubMed

Japanese encephalitis (JE) is numerically the most important global cause of encephalitis and so far confirmed to have caused major epidemics in India. Most of the reported studies have been in children. This largest study involving only adults, belonging to four epidemics, is being reported from Gorakhpur. The aim of this study is to detail the acute clinical profile (not viral) outcome and to classify the sequelae at discharge. This prospective study involved 1,282 adult patients initially diagnosed as JE admitted during the epidemics of 1978, 1980, 1988, and 1989, on identical clinical presentation and CSF examination. In the meantime, the diagnosis of JE was confirmed by serological and/or virological studies in only a representative number of samples (649 of 1,282 cases). Eighty-three left against medical advice (LAMA) at various stages, so 1,199 of 1,282 were available for the study. Peak incidence of [1,061 of 1,282 (83%)] of clinically suspected cases was from September 15 to November 2. Serum IgM and IgG were positive in high titers in 50.87% (330 of 649) and IgM positive in CSF in 88.75% (109 of 123) of the cases. JE virus could be isolated from CSF and brain tissue in 5 of 5 and 4 of 5 samples, respectively. Altered sensorium (AS) in (96%), convulsions (86%), and headache (85%) were the main symptoms for hospitalization by the third day of the onset. Other neurological features included hyperkinetic movements in 593 of 1,282 (46%)-choreoathetoid in 490 (83%) and bizarre, ill-defined in 103 (17%). The features of brain stem involvement consisted of opsoclonus (20%), gaze palsies (16%), and pupillary changes (48%) with waxing and waning character. Cerebellar signs were distinctly absent. Dystonia and decerebrate rigidity was observed in 43 and 6%, respectively, paralytic features in 17% and seizures in 30%. Many non-neurological features of prognostic importance included abnormal breathing patterns (ABP) (45%), pulmonary edema (PO) (33%), and upper gastrointestinal hemorrhage (UGIH) (16%). Injection dexamethasone was used in 1978 in all 208 cases, including 21 of PO. Patients were later randomized alternately in dexa and non-dexa groups. Forty-six cases of PO from the non-dexa group were transferred to the dexa group as an ultimate life-saving measure. Thus, it was administered in 737 of 1,199 patients including 529 patients from the later epidemics in doses of 4 mg IV every 8 h for 7 days. Of 1,199, 462 did not receive it. There was no significant difference in mortality (p > 0.05) between the dexa (42.47%) and the non-dexa group (42.86%). All PO cases expired; so after the exclusion of the PO cases from dexa group, the difference of 6.14% (42.86 and 36.72) became significant (p < 0.01) (511 of 1,199 (43%) expired, [320 of 511 (63%) died within 3 days of hospitalization]). Out of a total of 1,199 patients treated, 688 (57%) were discharged; 23 of 688 (3%) without any sequelae and 665 of 688 (97%) with neuropsychiatric deficits classified into nine groups. During the four epidemics, the diagnosis of JE was basically on identical clinical presentation of acute encephalitic syndrome (AES) consisting of (1) abrupt onset of fever, headache, and AS, (2) dystonias and various movement disorders, (3) opsoclonus and gaze palsies, (4) CSF findings, and (5) the presence of residual neuropsychiatric and neurological features in the survivors. PMID:21678123

Sarkari, N B S; Thacker, A K; Barthwal, S P; Mishra, V K; Prapann, Shiv; Srivastava, Deepak; Sarkari, M

2012-01-01

50

Books and Book Chapters Berlo, K., Gardner, J.E., and Blundy, J.D., Timescales of Magma Degassing, in, Dosseto,  

E-print Network

and Submitted: * Andrews, B.J., and Gardner, J.E., Turbulent dynamics of partially collapsing volcanic eruption 14 C yr BP eruption of Ksudach Volcano, Kamchatcka, Russia, Earth Planet. Sci. Lett., in review the White Pumice Plinian eruption of Popocatépetl volcano, Mexico, J. Volcanol. Geotherm. Res., in review

Yang, Zong-Liang

51

Nanometre resolution tracking of myosin-1b motility M.I. Wallace, C. Batters, L.M. Coluccio and J.E. Molloy  

E-print Network

Nanometre resolution tracking of myosin-1b motility M.I. Wallace, C. Batters, L.M. Coluccio and J.E. Molloy Abstract: The movement produced by a small number of myosin molecular motors was measured individual myosin motors and the actin filament. Dependence of sliding velocity upon filament length

Wallace, Mark

52

Injection locking of a fiber-coupled laser diode array M.A. Humphrey, J.E. Massey, D.F. Phillips, and R.L. Walsworth  

E-print Network

1 Injection locking of a fiber-coupled laser diode array M.A. Humphrey, J.E. Massey, D.F. Phillips, fiber-coupled laser diode array was significantly narrowed by injection locking. Up to 72% of the 1 THz intrinsic to the fiber- coupling of the slave laser reduced the injection-locked gain to less than unity

Walsworth, Ronald L.

53

Ceballos, G., List, R., Davidson, A., Fredrickson, E.L., Sierra Corona, R., Martinez, L., Herrick, J.E., Pacheco, J. 2009. Grassland in the Borderlands. Understanding coupled  

E-print Network

., Herrick, J.E., Pacheco, J. 2009. Grassland in the Borderlands. Understanding coupled natural-human systems of Arizona Press. p. 188-203. Chapter 12 Grasslands in the borderlands: understanding coupled human, Rodrigo Sierra, Lourdes Martínez, Jeff E. Herrick and Jesús Pacheco In a Nutshell Grassland ecosystems

54

Remarks on Separating Words Erik D. Demaine 1 , Sarah Eisenstat 1 , Je#rey Shallit 2 , and David A. Wilson 1  

E-print Network

Remarks on Separating Words Erik D. Demaine 1 , Sarah Eisenstat 1 , Je#rey Shallit 2 , and David A. Wilson 1 1 MIT Computer Science and Artificial Intelligence Laboratory, 32 Vassar Street, Cambridge, MA words problem can be viewed as an inverse of a classical problem from the early days of automata theory

Demaine, Erik

55

Gambogic acid induces mitochondria-dependent apoptosis by modulation of Bcl-2 and Bax in mantle cell lymphoma JeKo-1 cells  

PubMed Central

Objective To study the mechanisms in gambogic acid (GA) -induced JeKo-1 human Mantle Cell Lymphoma cell apoptosis in vitro. Methods The proliferation of GA-treated JeKo-1 cells was measured by CCK-8 assay and Ki-67 immunocytochemical detection. Apoptosis, cell cycle and mitochondrial membrane potential were measured by flow cytometric analysis. Caspase-3, -8 and -9 were detected by colorimetric assay. Bcl-2 and Bax were analyzed by Western blotting. Results GA inhibited cell growth in a time- and dose- dependent manner. GA induces apoptosis in JeKo-1 cells but not in normal bone marrow cells, which was involved in reducing the membrane potential of mitochondria, activating caspases-3, -8 and -9 and decreasing the ratio of Bcl-2 and Bax without cell cycle arresting. Conclusions GA induced apoptosis in human MCL JeKo-1 cells by regulating Bcl-2/Bax and activating caspase-3, -8 and -9 via mitochondrial pathway without affecting cell cycle. PMID:23592899

Xu, Jingyan; Zhou, Min; Wang, Jing; Zhang, Qiguo; Xu, Yong; Xu, Yueyi; Zhang, Qian; Xu, Xihui; Zeng, Hui

2013-01-01

56

Jezik, identiteta in miselne meje (Pou?evanje o jeziku in »vzhodni Evropi« na ameriški univerzi)  

E-print Network

?inskih jezikov brez pisne tradicije v knji?ne jezike. V #1;asu, ko prevladuje nazor #1;rno-bele moralnosti in se vse zadeve reducirajo na eno ali drugo ? tertium non datur ? je pou#1;no prikazati primer moralno sivega, npr. vzpon in padec jidi?sa v Evropi...

Greenberg, Marc L.

2005-01-01

57

Prediction of Turbulence-Generated Noise in Unheated Jets. Part 1; JeNo Technical Manual (Version 1.0)  

NASA Technical Reports Server (NTRS)

The model-based approach, used by the JeNo code to predict jet noise spectral directivity, is described. A linearized form of Lilley's equation governs the non-causal Green s function of interest, with the non-linear terms on the right hand side identified as the source. A Reynolds-averaged Navier-Stokes (RANS) solution yields the required mean flow for the solution of the propagation Green s function in a locally parallel flow. The RANS solution also produces time- and length-scales needed to model the non-compact source, the turbulent velocity correlation tensor, with exponential temporal and spatial functions. It is shown that while an exact non-causal Green s function accurately predicts the observed shift in the location of the spectrum peak with angle as well as the angularity of sound at low to moderate Mach numbers, the polar directivity of radiated sound is not entirely captured by this Green s function at high subsonic and supersonic acoustic Mach numbers. Results presented for unheated jets in the Mach number range of 0.51 to 1.8 suggest that near the peak radiation angle of high-speed jets, a different source/Green s function convolution integral may be required in order to capture the peak observed directivity of jet noise. A sample Mach 0.90 heated jet is also discussed that highlights the requirements for a comprehensive jet noise prediction model.

Khavaran, Abbas; Bridges, James; Georgiadis, Nicholas

2005-01-01

58

[Oviposition, development, and reproduction of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) fed on different hosts of economic importance].  

PubMed

The host selection for oviposition by Spodoptera frugiperda (J.E. Smith) among corn, millet, cotton and soybean, and its relationship with the biological characteristics were investigated. Free and non-choice tests for oviposition using plots containing five plants each, from each host in plastic greenhouse, resulted in similar oviposition preference among the host plants. In addition, selected biological characteristics of S. frugiperda were determined in the laboratory with larvae feeding on host leaves, and the combination of leaf and cotton boll. Neonate larvae exhibited low success of colonization on cotton boll compared to the leaves of all other hosts. Spodoptera frugiperda fed only on cotton bolls exhibited longer larval and pupal development, and longer adult life span; however with similar egg production. Larvae fed cotton leaves during six days and then transferred to cotton bolls, however, exhibited development and reproduction similar to those reared on corn or only on cotton leaves. Therefore, the variations on immature stages of S. frugiperda were not related with host selection for oviposition which was similar among the studied hosts. Based on our data, the millet as a winter, rotational, and cover crop is a potential host for S. frugiperda, while leaves and cotton bolls were diets of intermediate suitability as compared to corn and soybean leaves. PMID:21271070

Barros, Eduardo M; Torres, Jorge B; Bueno, Adeney F

2010-01-01

59

Cutaneous delivery of a live, attenuated chimeric flavivirus vaccine against Japanese encephalitis (ChimeriVax)-JE) in non-human primates.  

PubMed

Flaviviral diseases such as yellow fever, Japanese encephalitis (JE) and dengue hemorrhagic fever cause enormous morbidity and mortality worldwide. There is an urgent need for alternative technologies for mass vaccination against these and other diseases, particularly in the developing world. Here, we administered a live attenuated, chimeric JE vaccine (ChimeriVax)-JE) to nonhuman primates by skin microabrasion and intradermal delivery using microneedles. Both cutaneous delivery methods induced mild viremia similar in magnitude to that observed following subcutaneous (SC) injection. The duration of viremia induced by cutaneous delivery (5-7 days), however, was substantially longer than via SC (0-3 days). In addition, mean neutralizing antibody titers in cutaneous delivery groups were up to 7-fold greater than via SC injection. There were no safety issues identified and both cutaneous delivery methods appeared to be well tolerated. Thus, cutaneous delivery may represent a minimally-invasive alternative approach for flavivirus vaccines that more closely resembles the natural route of viral infection. PMID:17012854

Dean, Cheryl H; Alarcon, Jason B; Waterston, Andrea M; Draper, Ken; Early, Richard; Guirakhoo, Farshad; Monath, Thomas P; Mikszta, John A

2005-01-01

60

OPERATIVNO ZDRAVLJENJE HALUKSA VALGUSA - VPLIV TREH VRST OSTEOTOMIJ NA INTERMETATARZALNI, HALUKS VALGUS IN DISTALNI METATARZALNI KOT OPERATIVE TREATMENT OF HALLUX VALGUS - THE EFFECT OF THREE OSTEOTOMY TYPES ON THE FIRST METATARSAL, HALLUX VALGUS AND METATARSAL DISTAL ARTICULATION ANGLES  

Microsoft Academic Search

Izvle?ek Izhodiš?a Moderno zdravljenje valgusnih deformacij palca omogo?a u?inkovito stopenjsko obravnavo glede na velikost deformacije in objektivno ocenjevanje rezultatov kljub še ve- dno prisotnemu preživetemu o?itku naklju?nosti in neuspešnosti. Zanimal nas je vpliv treh najpogostejših osteotomij na tri najpogostejše kazalce, še posebej vpliv proksimalne osteotomije na naklonski kot glavice prve metatarzalne kosti. Bolniki in metode Oblikovali smo tri skupine bolnikov

Andrej Prlja; Ivica Avberšek-Lužnik

61

3. Common Lisp: zkladn v!bava Studijni cile: Common Lisp je jazyk s velmi jednoduchou syntax, kterou jsme mohli  

E-print Network

pojmenovávající makro, v'sledek v$etn" p#ípadného vedlej%ího efektu závisí na speciálním operátoru Op, p#í- padn standardu definice a p#íklady pou(ití (v$etn" souvislosti se speciálním operátorem if a makrem cond) lze

Krupka, Michal

62

[Artificial diet for rearing Doru luteipes (Scudder) (Dermaptera: Forficulidae), a predator of the fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)].  

PubMed

A new technique involving an artificial diet and an artificial substrate for oviposition for the rearing of the predator Doru luteipes (Scudder) is suggested. Both adults and nymphs were maintained in petri dishes containing a transparent piece of soda straw filled with moistened cotton and the corresponding food for the biossays. The following treatments were tested: eggs of Spodoptera frugiperda (J.E. Smith), Diatraea saccharalis Fabricius (Lepidoptera: Pyralidae) and Anagasta kuehniella Zeller (Lepidoptera: Pyralidae); insect pupae meal (FPI); Apis mellifera L. (Hymenoptera: Apidae) commercial pollen (PC); FPI + PC, and FPI + cattail pollen [Typha angustifolia L. (Thyphaceae)]. Each treatment had 50 replicates, and food was offered in excess. Treatments consisting of insect pupae meal (FPI), FPI + commercial pollen (PC), and FPI + cattail pollen resulted in nymphal development of 32, 29, and 29 days, with 83, 90 and 100% survival, respectively, and were superior to the PC treatment, with values of 37 days and 67% survival observed for insects reared on commercial pollen. Treatments that included insect pupae flour, either alone or mixed with pollens, were similar to control (S. frugiperda eggs). We conclude that the artificial diets tested and rearing technique are suitable for the artificial rearing of D. luteipes in laboratory conditions. PMID:17607467

Pasini, Amarildo; Parra, José R P; Lopes, Janaína M

2007-01-01

63

Imidacloprid affects the functional response of predator Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) to strains of Spodoptera frugiperda (J.E. Smith) on Bt cotton.  

PubMed

Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) is one of the most common asopine species in the neotropical region and its occurrence was reported in several countries of South and Central America, as an important biological control agent for many crops. This study was carried out to identify the imidacloprid impacts on the functional response of predator P. nigrispinus fed on Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) strain resistant to lambda-cyhalothrin, on Bt cotton expressing Cry1Ac (Bollgard(®)). Spodoptera frugiperda larvae were used in the following conditions: resistant (1) and susceptible (2) strains to lambda-cyhalothrin fed on Bollgard(®) cotton leaves (DP 404 BG); and resistant (3) and susceptible (4) strains to lambda-cyhalothrin fed on non-genetically modified cotton leaves (cultivar DP4049). The predatory behavior of P. nigrispinus was affected by imidacloprid and the type II asymptotic curve was the one that best described the functional response data. Handling time (T h ) of predator females did not differ among treatments in the presence of imidacloprid. The attack rate did decrease, however, due to an increase in the density of larvae offered. Regardless of the treatment (S. frugiperda strain or cotton cultivar), the predation of P. nigrispinus females on S. frugiperda larvae was significantly lower when exposed to imidacloprid, especially at a density of 16 larvae/predator. The predation behavior of P. nigrispinus on S. frugiperda larvae is affected by the insecticide imidacloprid showing that its applications should be used in cotton crop with caution. PMID:24352830

Malaquias, J B; Ramalho, F S; Omoto, C; Godoy, W A C; Silveira, R F

2014-03-01

64

Introduction Je pense, donc je suis--Rene Descartes.  

E-print Network

and coordinated movements of our bodies, but also processes sensory signals for appropriately responding of action potentials is at most 102 m/sec in myelinated axons, whereas that of electronic circuits can computations are readily tractable. One such type of the problems is sensory inference. Taking acoustic signal

Zador, Anthony

65

lerarenopleiding Start je binnenkort met een masteropleiding of behaal je binnenkort je master  

E-print Network

gericht is op de vorming van toekomstige leraren secundair onderwijs. Er is evenwel ook aandacht voor een bredere educatieve vorming met oog op onderwijsopdrachten in het hoger onderwijs, het sociaalcultureel gebaseerd op de basis competenties voor de leraar secundair onderwijs die bij decreet zijn vastgelegd. De

Gent, Universiteit

66

lerarenopleiding Start je met een masteropleiding of behaal je binnenkort je masterdiploma  

E-print Network

vorming van toekomstige leraren secundair onderwijs. Er is evenwel ook aandacht voor een bredere educatieve vorming met oog op onderwijsopdrachten in het hoger onderwijs, het sociaalcultureel vormingswerk de basis competenties voor de leraar secundair onderwijs die bij decreet zijn vastgelegd. De

Gent, Universiteit

67

Vpliv Agusti?eve publicisti?ne dejavnosti na razvoj knjižne prekmurš?ine  

E-print Network

literarne ambicije so bile sprva opazne v madžarski leposlovni publicistiki (prim. Barbari? 1985), kasneje pa tudi v ?asopisu Prijatel. V madžarš?ino je prevajal nemško in francosko leposlovje (Poskusna gesla za novi SBL 2000: 19), v poltedniku za kulturo... zamejevati z let- nicama 1875 in 1879 (prim. Szinnyei 1891: 292), natan?en podatek o njegovem prenehanju izhajanja pa je prvi podal šele Vilko Novak, ki je navedel, da je ?asopis nazadnje izšel »dva dni pred urednikovo smrtjo« (Novak 1976: 82), torej 15...

Ul?nik, Natalija

2009-01-01

68

Napovedna veljavnost mature za ötudij psihologije  

Microsoft Academic Search

Povzetek: V zadnjih nekaj letih se je z uvedbo maturitetnega preizkusa kot zakljuka srednje öole in selekcijskega postopka za vpis na univerzo marsikaj spremenilo. Avtor je ûelel raziskati odnos med maturo kot selekcijskim postopkom za vpis na ötudij psihologije na Univerzi v Ljubljani in selekcijskim postopkom, ki je temu namenu sluûil prej ñ sprejemnim izpitom. V srediöu zanimanja je predvsem

C ANKAR

2000-01-01

69

NaF Documentation  

Cancer.gov

The NaF documentation files are presented in Adobe Acrobat or Word files. PDF Generic Documentation for NaF PDF file Word Generic Documentation for NaFWord file Contact Paula M. Jacobs, Ph.D., 301-435-9181, jacobsp@mail.nih.gov, for information. Print

70

----------Forwarded message ----------From: Yi-Je Lim  

E-print Network

CSAIL, Hstar seeks to improve the efficacy and quality of care for patients through the use of robotic, 2011 at 9:50 AM Subject: [robotics-worldwide] Cambridge - MA. Robotics Engineer Positions: Senior Mechanical Design, Electrical, Control, and Internship To: robotics-worldwide@usc.edu Multiple full

Plotkin, Joshua B.

71

Hoe leer je in en met netwerken?  

Microsoft Academic Search

Sloep, P. B., Rajagopal, K., Fetter, S., Koper, R., Gijselaers, J., Kalz, M., Wolff, C., Brand-Gruwel, S., Berkhout, J., Storm, J., & Blankestein, H. (2012, 9 maart). Leren in en met netwerken. Masterclass in de OpenU community. Open universiteit, Heerlen, Nederland. Beschikbaar op http:\\/\\/portal.ou.nl\\/web\\/masterclass-netwerkleren\\/introductie\\/

Peter Sloep; Kamakshi Rajagopal; Sibren Fetter; Rob Koper; Jérôme Gijselaers; Marco Kalz; Charlotte Wolff; Saskia Brand-Gruwel; Jeroen Berkhout; Jeroen Storm; Herbert Blankestein

2012-01-01

72

Milli Robotics for Endoscopy Je Wendlandt1  

E-print Network

and operate in the gastrointestinal tract. Current instruments lack #12;ne motion control and dexterity performs operations and inspections in the gastrointestinal tract. Endoscopy's primary bene#12;t Procedures 5 3.1 Upper Gastrointestinal (GI) Endoscopy : : : : : : : : : : : : : : : : : : : : : 8 3

Wendlandt, Jeff

73

God's Calling Je rey D. Oldham  

E-print Network

repeatedly appearing note, voice, George Burns God loves you and you have all the tools needed to solve all or love, not on subsequent actions or theology. If we did the latter, we would have to study the entire Bible and more! It is your responsibility to #12;nd the patterns and determine how what you learn

Pratt, Vaughan

74

Je voyage au Canada (Travelling to Canada).  

ERIC Educational Resources Information Center

Designed for use in a bilingual program, this basic French reader centers around a trip to Canada. It contains reading selections, two short poems, the words and music of a song entitled "La cigale et la fourmi," and several oral and written exercises. (PMP)

Dube, Normand

75

La fin du je?ne?  

PubMed Central

Résumé Objectif Présenter une mise à jour sur l’utilité clinique de ne pas être à jeun par rapport à l’être pour l’analyse des lipides dans le but d’améliorer l’observance par les patients, leur sécurité et l’évaluation clinique dans les tests du cholestérol. Qualité des données Les recommandations sont classées comme étant fondées sur des données probantes fortes, acceptables ou faibles (conflictuelles ou insuffisantes), selon les classifications adoptées par le Groupe d’étude canadien sur les soins de santé préventifs. Message principal Le dépistage de la dyslipidémie comme facteur de risque de coronaropathie et la prescription de médicaments hypolipidémiants sont des activités importantes en soins primaires. De récentes données probantes remettent en question la nécessité d’être à jeun pour la mesure des lipides. Dans des études sur la population, le cholestérol total, le cholestérol à lipoprotéines de haute densité et le cholestérol à lipoprotéines autres qu’à haute densité variaient tous d’en moyenne 2 % à jeun. Pour un dépistage de routine, la mesure du cholestérol sans être à jeun est maintenant une option de rechange raisonnable à l’analyse à jeun. Pour les patients diabétiques, l’exigence d’être à jeun peut représenter un important problème de sécurité en raison des possibilités d’hypoglycémie. Pour la surveillance des triglycérides et du cholestérol à lipoprotéines de basse densité chez les patients qui prennent des médicaments hypolipidémiants, le jeûne devient important. Conclusion Être à jeun pour la détermination routinière des niveaux lipidiques est largement inutile et il est improbable que le jeûne influence la stratification du risque clinique chez le patient, tandis que la mesure sans être à jeun pourrait améliorer l’observance par le patient et sa sécurité. PMID:25316760

Naugler, Christopher; Sidhu, Davinder

2014-01-01

76

The dissolution of synthetic Na-boltwoodite in sodium carbonate solutions  

NASA Astrophysics Data System (ADS)

Uranyl silicates such as uranophane and Na-boltwoodite appear to control the solubility of uranium in certain contaminated sediments at the US Department of Energy Hanford site [Liu, C., Zachara, J.M., Qafoku, O., McKinley, J.P., Heald, S.M., Wang, Z. 2004. Dissolution of uranyl microprecipitates in subsurface sediments at Hanford Site, USA. Geochim. Cosmochim. Acta68, 4519-4537.]. Consequently, the solubility of synthetic Na-boltwoodite, Na(UO 2)(SiO 3OH) · 1.5H 2O, was determined over a wide range of bicarbonate concentrations, from circumneutral to alkaline pH, that are representative of porewater and groundwater compositions at the Hanford site and calcareous environments generally. Experiments were open to air. Results show that Na-boltwoodite dissolution was nearly congruent and its solubility and dissolution kinetics increased with increasing bicarbonate concentration and pH. A consistent set of solubility constants were determined from circumneutral pH (0 added bicarbonate) to alkaline pH (50 mM added bicarbonate). Average logKspo=5.86±0.24 or 5.85 ± 0.0.26; using the Pitzer ion-interaction model or Davies equation, respectively. These values are close to the one determined by [Nguyen, S.N., Silva, R.J., Weed, H.C., Andrews, Jr., J.E., 1992. Standard Gibbs free energies of formation at the temperature 303.15 K of four uranyl silicates: soddyite, uranophane, sodium boltwoodite, and sodium weeksite. J. Chem. Thermodynamics24, 359-376.] under very different conditions (pH 4.5, Ar atmosphere).

Ilton, Eugene S.; Liu, Chongxuan; Yantasee, Wassana; Wang, Zheming; Moore, Dean A.; Felmy, Andrew R.; Zachara, John M.

2006-10-01

77

Transitions in Na IIITransitions in Na III  

Microsoft Academic Search

The work of Blackford and Hibbert (1994 At.Data Nucl.Data Tables58 101-64) has been extended to provide oscillator strengths and transition probabilities for J?J' transitions amongst n = 2 and n = 3 levels of Na III. Configuration interaction wavefunctions, obtained with the code CIV3 in the Breit-Pauli approximation, and with additional correlation effects, have been constructed. Further semi-empirical corrections to

D. McPeake; A. Hibbert

2000-01-01

78

Results from NA57  

E-print Network

The NA57 experiment has measured the production of strange and multi-strange hadrons in heavy-ion collisions at the CERN SPS. After briefly introducing the NA57 apparatus and analysis procedures, we present recent results on strangeness enhancement in Pb-Pb relative to p-Be collisions, on the study of the m_T distributions of strange particles, and on central-to-peripheral nuclear modification factors in Pb-Pb collisions at top SPS energy.

Andrea Dainese

2005-09-30

79

Savivert?s ugdymas antrojoje vaikyst?je ir paauglyst?je.  

E-print Network

??The level of self-esteem regulates one’s behavior in various life situations and influences interpersonal relations qreatly. Self-esteem defines everyone’s psychological self-feeling. The development of self-esteem… (more)

Jarušaitien?,; Daina

2006-01-01

80

Blackboard is de elektronische leeromgeving van de TU Delft. Hier moet je je voor alle cursussen  

E-print Network

Aanmelden tentamens In het Onderwijs- en Examenreglement (OER) staan de geldende procedures, rechten en plichten met betrekking tot het onderwijs en de examens. In de Uitvoeringsregeling (UR) staat het volledige voor de organisatie van het onderwijs. Voor dringende problemen: g.j.broekman@tudelft.nl, kamer A217, T

81

Hru vytvo#il Manfred Schuling v roce 1972. Poch#zel z chud## rodiny, a proto si s#m vyr#b#l hra#ky a hry. ##k#: #To je nejlep## #kola pro tv#rce  

E-print Network

-- 4 D#lka hry: ?? Hrac# pot#eby: Hrac# pl#n, 121 hrac#ch kamen# s hodnotami 1 -- 6 bod#, s##ek na souvisl# kombinace, kter# d#vaj# v #ad# sou#et 10, 11 nebo 12 bod#. V#choz# pozice: Hr##i si vylosuj# po, sou#et bod# v#ak nesm# p#ekro#it 12. Pokud prvn# hr## nedos#hne 10 bod#, prob#h# cel# losov## znova

Fiala, Jirí

82

Na Cauda do Cometa  

NASA Astrophysics Data System (ADS)

Quando viam um cometa, os antigos gregos imaginavam uma estrela com uma vasta cabeleira. Não à toa, a palavra deriva do termo koma, que significa cabelo. Constituídos por fragmentos de gelo e gases, os cometas possuem um núcleo sólido, que pode ter vários quilômetros de diâmetro, e uma cauda que sempre aponta na direção contrária ao Sol, devido aos ventos solares. Graças à aparência de pontos luminosos em movimento (ao contrário de outros astros, que parecem estáticos), esses corpos celestes foram interpretados por diferentes povos com muito misticismo, inspirando mitos tanto de boas-novas como de maus presságios. Conheça algumas dessas histórias:

Voelzke, M. R.

2009-01-01

83

A deontológiai prioritás: Gondolatrokonság Ny. F. Fjodorov és a »korai szlavofilizmus« képvisel?i között  

E-print Network

N. F. Fedorov (1829–1903) je eden najbolj izvirnih ruskih mislecev vseh ?asov, kljub temu da je bil v sovjetskem obdobju prakti?no nepoznan. Zgodnji slovanofilski misleci, kot so na primer Aleksei Homyakov, Ivan Kireevski ...

Medzibrodszky, Alexandra

2014-02-03

84

Sociolinguistic and Geolinguistic Variation in the Basque language  

E-print Network

V prispevku so predstavljeni prvi rezultati Socio- in geolingvisti?nega atlasa baskovskega jezika – EAS. Raziskovalni projekt je bil predstavljen na 5. konferenci SIDG, ki je bila v Bragi. Predstavljamo nekatere podatke, ...

Aurrekoetxea, Gotzon

2010-01-01

85

Temeljna na?ela i postupci u pretvaranju sirove dijalektološke gra?e u znanstveni rje?nik  

E-print Network

. ? L?i??p? se d?rž? d?k t?? d??jdem?. 3. ponositi se. ? Drž?i se n??kaj, a n?i??ma n?t? za kr?uh!14 U sva 3 rje?ni?ka ?lanka »neo?ekivana« zna?enja su kod refleksivnog gla- gola. 2.4 Nužno je razgrani?iti homonimiju i polisemiju,15 što je u mnogim slu?aje.... upropastiti imovinu. ? Pr?odal je z???m?? ? za ??as je ?uspel sp?i?kat? s??? p??neze. v. ra(e)s(f)p?r?kat? i sf(p)?r?kat? 2. vulG. odbaciti nekoga. ? D?k me tr??bal, sam m? d?ober b?il, a v??? me sp?i?kal da m? v??? ne tr??bam. ZMISLITI (Se) [zm?isl?t? (se...

Blažeka, ?uro

2010-02-01

86

NNJ06JE86C DRD18 JS2011010 NNJ06JE86C DRD18 JS2011010 NNJ06JE86C DRD18 JS2011010  

E-print Network

and as a broad indication of the failure rate. The metrics in the legend show the frequency of early termination PLSS Primary Life Support System DTO Developmental Test Objective LiOH Lithium Hydroxide RCC Reinforced-Workstation SAFER Simplified Aid for EVA Rescue HCM Hand Control Module NSI NASA Standard Initiator STS Space

87

Blackboard is de elektronische leeromgeving van de TU Delft. Hier moet je je voor alle cursussen aanmelden. Ook vind je er  

E-print Network

HB 05.290, T015-27 84639. Joost de Groot Studentenportal TNW In het Onderwijs- en Examenreglement (OER) staan de geldende procedures, rechten en plichten met betrekking tot het onderwijs en de examens

88

Blackboard is de elektronische leeromgeving van de TU Delft. Hier moet je je voor alle cursussen aanmelden. Ook vind je er  

E-print Network

. Rooster Aanmelden tentamens In het Onderwijs- en Examenreglement (OER) staan de geldende procedures, rechten en plichten met betrekking tot het onderwijs en de examens. In de Uitvoeringsregeling (UR) staat

89

Obesidade infantil: excessos na sociedade  

Microsoft Academic Search

1 Resumo: A obesidade representa um grave problema de saúde pública, caracterizada por um contexto de epidemia mundial. A especificidade da obesidade infantil se estabelece a partir das conseqüências físicas e emocionais da doença, além da elevação dos riscos da moléstia na vida adulta. Através de encontros grupais com familiares de crianças obesas, identificaram-se aspectos relevantes na vida social que

Andréia Mendes dos Santos

90

Challenges for Na-ion Negative Electrodes  

E-print Network

Na-ion batteries have been proposed as candidates for replacing Li-ion batteries. In this paper we examine the viability of Na-ion negative electrode materials based on Na alloys or hard carbons in terms of volumetric ...

Chevrier, V. L.

91

Dkazy nerovnost pomoc metod vzanch extrm Vta 1 (AG nerovnost). Nech n N, a1, . . . , an 0. Potom  

E-print Network

. Protoºe na hranici mnoºiny M je ~f = 0 a na zbytku je pouze jediný podez°elý bod s hodnotou ~f(C, . . . , C) = Cn 0, je tento bod nutn¥ bodem maxima ~f (a tedy i f) vzhledem k M. Je maxM f = f(C, . . . , C a na zbytku je pouze jediný podez°elý bod x s hodnotou f(x) = m i=1 ap i 1 p C m i=1 ap i m i=1 a q q-1

Johanis, Michal

92

Asmens privatumas elektronin?je darbo vietoje: teisiniai aspektai.  

E-print Network

??Substantial technology developments over the past two decades have dramatically transformed today’s workplaces. It became easier to perform work functions. However, these changes made employers… (more)

Urbonait?,; Milda

2006-01-01

93

Controlled photomosaic map of Europa Je 15 M CMN  

USGS Publications Warehouse

This sheet is one in a series of maps of the Galilean satellites of Jupiter at a nominal scale of 1:15,000,000. This series is based on data from the Galileo Orbiter Solid-State Imaging (SSI) camera and the Voyager 1 and 2 spacecraft.

2002-01-01

94

Low Energy Transfer to the Moon , J.E. Marsden  

E-print Network

approximate the Sun-Earth-Moon-spacecraft 4-body system as two 3-body systems. Using the invariant manifold trajectories from the Earth which execute ballistic capture at the Moon. The techniques used in the design and construction of this trajectory may be applied in many situations. Keywords: earth-moon transfer, invariant

Koon, Wang Sang

95

Je prends connaissance du Code d'honneur  

E-print Network

http://www.direction.umontreal.ca/secgen/pdf/reglem/francais/sec_30/ens30_3.pdf . J'assume mes promotion d'activités commerciales ou de sollicitation. Détails à http://www.direction.umontreal.ca/secgen/pdf/reglem/francais

Montréal, Université de

96

Gibt es heute mehr epiphytische Moose als je zuvor?  

Microsoft Academic Search

Zusammenfassung: Die rapide Arten- als auch Mengenzunahme der Moosepiphyten in den letzten Jahren gerade in Städten stimulierte einen Vergleich der Häufigkeit der Arten früher (19. und 20. Jahrhundert) und den letzten 10 Jahren. Es zeigte sich, dass - hauptsächlich bezogen auf das Rheinland bzw. Nordrhein-Westfalen in letzter Zeit mehr Nachweise gemacht wurden als in den 150 Jahren zuvor. Grund ist

Jan-Peter Frahm

97

Techniques to Foster Drum Machine Expressivity Je A. Bilmes  

E-print Network

will call it, tatum3. Perceiving the tatum does not necessarily implya conscious ticking in the mind, like's thought when needed. Perceiving the tatum im- plies that the listener or performer is judging

Bilmes, Jeff

98

Je pense donc je fais: transcranial direct current stimulation modulates brain oscillations associated with motor imagery and movement observation  

PubMed Central

Motor system neural networks are activated during movement imagery, observation and execution, with a neural signature characterized by suppression of the Mu rhythm. In order to investigate the origin of this neurophysiological marker, we tested whether transcranial direct current stimulation (tDCS) modifies Mu rhythm oscillations during tasks involving observation and imagery of biological and non-biological movements. We applied tDCS (anodal, cathodal, and sham) in 21 male participants (mean age 23.8 ± 3.06), over the left M1 with a current of 2 mA for 20 min. Following this, we recorded the EEG at C3, C4, and Cz and surrounding C3 and C4 electrodes. Analyses of C3 and C4 showed significant effects for biological vs. non-biological movement (p = 0.005), and differential hemisphere effects according to the type of stimulation (p = 0.04) and type of movement (p = 0.02). Analyses of surrounding electrodes revealed significant interaction effects considering type of stimulation and imagery or observation of biological or non-biological movement (p = 0.03). The main findings of this study were (1) Mu desynchronization during biological movement of the hand region in the contralateral hemisphere after sham tDCS; (2) polarity-dependent modulation effects of tDCS on the Mu rhythm, i.e., anodal tDCS led to Mu synchronization while cathodal tDCS led to Mu desynchronization during movement observation and imagery (3) specific focal and opposite inter-hemispheric effects, i.e., contrary effects for the surrounding electrodes during imagery condition and also for inter-hemispheric electrodes (C3 vs. C4). These findings provide insights into the cortical oscillations during movement observation and imagery. Furthermore, it shows that tDCS can be highly focal when guided by a behavioral task. PMID:23761755

Lapenta, Olivia M.; Minati, Ludovico; Fregni, Felipe; Boggio, Paulo S.

2013-01-01

99

Sedmera Vvoj komorovho myokardu Funkce orgnu je podmnna jeho strukturou. Toto tvrzen nabv zvlstn vznam u srdce, jez je jednm  

E-print Network

development, and during its morphogenesis must support the circulatory demands of the growing organism in the adult heart. Ventricular conduction system serves above all the coordinated spread of electrical and functional deployment of the various components of cardiac conuction system in the chick embryo, including

Tebbens, Jurjen Duintjer

100

Blackboard is de elektronische leeromgeving van de TU Delft. Hier moet je je voor alle cursussen aanmelden.  

E-print Network

Onderwijs- en Examenreglement (OER) staan de geldende procedures, rechten en plichten met betrekking tot het onderwijs en de examens. In de Uitvoeringsregeling (UR) staat het volledige programma van de opleiding organisatie van het onderwijs. Voor dringende problemen: g.j.broekman@tudelft.nl, kamer A217, T015-27 82128

101

Determination of the (Na+) Sternheimer antishielding factor by 23Na NMR spectroscopy on sodium oxide chloride, Na3OCl.  

PubMed

The (Na+) Sternheimer antishielding factor gammainifinity (Na+) was determined by 23Na NMR spectroscopy on sodium oxide-chloride, Na3OCl. The quadrupolar coupling constant of the sodium ion in Na3OCI was determined to QCC = 11.34 MHz, which presents the largest coupling constant of a sodium nucleus observed so far. Applying a simple point charge model, the largest principal value of the electric field gradient at the sodium site was calculated to V(zz) = -6.76762 x 10(20) V/m2. From these values we calculated the (Na+) Sternheimer antishielding factor to gammainifinity (Na+)= -5.36. In sodium oxide, Na2O, we observed an isotropic chemical shift of deltaCS = 55.1 ppm, referenced to 1 M aqueous NaCI (delta = 0 ppm). PMID:10928632

Klösters, G; Jansen, M

2000-07-01

102

Original article Soybean impairs Na  

E-print Network

Original article Soybean impairs Na + -dependent glucose absorption and Cl ­ secretion in porcine -- Recent evidence indicates that soybean, which is widely used in animal nutrition, could directly alter of the study was to investigate the effect of three differently treated soybean prod- ucts on the glucose

Boyer, Edmond

103

BREAKFAST MENU (665 MG NA)  

E-print Network

) Garden Vegetable (80 MG) Beef Broth(140 MG) Cottage Cheese (410 MG) Tossed Salad (O MG) (Oil & Vinegar SODIUM (2000 GM NA) COLD ENTREES: Chicken Caesar Salad (440 MG) (Oil & Vinegar/Lowfat Italian EACH) Baked Potato Chips (170 MG) ACCOMPANIMENTS: (Limit 1 Starch 1 Veg) Green Beans(6 MG) Broccoli

Oliver, Douglas L.

104

NA61/SHINE ion program  

E-print Network

The Super Proton Synchrotron (SPS) at CERN covers one of the most interesting regions of the phase diagram (T - \\mu_{B}) of strongly interacting matter. The study of central Pb+Pb collisions by NA49 indicate that the threshold for deconfinement is reached already at the low SPS energies. Theoretical considerations predict a critical point of strongly interacting matter at energies accessible at the SPS. The NA61/SHINE experiment, a successor of the NA49 project, will study hadron production in p+p, p+A, h+A, and A+A reactions at various energies. The broad physics program includes the investigation of the properties of strongly interacting matter, as well as precision measurements of hadron spectra for the T2K neutrino experiment and for the Pierre Auger Observatory and KASCADE cosmic-ray projects. The main physics goals of the NA61/SHINE ion program are to study the properties of the onset of deconfinement at low SPS energies and to find signatures of the critical point of strongly interacting matter. To achieve these goals a broad range in the (T - \\mu_{B}) phase diagram will be covered by performing an energy (10A-158A GeV/c) and system size (p+p, B+C, Ar+Ca, Xe+La) scan. The first data for this 2-D scan were taken in 2009, i.e. p+p interactions at 20, 30, 40, 80, 158 GeV/c beam energy. This contribution will summarize physics arguments for the NA61/SHINE ion program, show the detector performance and present the current status of the experiment and plans for the next years.

Maja Mackowiak for the NA61 Collaboration

2010-09-06

105

, zadania na wiczenia, seria VI Rozkad ideaw w piercieniach noetherowskich.  

E-print Network

z nich. Przypomnienie: Niech M b¦dzie moduªem nad pier±cieniem A, wówczas Ass M to zbiór ideaªów przykªady. 2. Rozpatrzmy A-moduª M i jego dwa podmoduªy M1 i M2. (a) Poka», »e je±li M = M1 + M2 to Ass M Ass M1 Ass M2 ale niekoniecznie musi zachodzi¢ równo±¢. (b) Czy Ass M = Ass M1 Ass(M/M1)? Odpowied

Wisniewski, Jaroslaw A.

106

Melting of the Na layers in solid Na0.8CoO2.  

PubMed

Data of 23Na NMR spectra and relaxation measurements are interpreted as suggesting that, upon increasing temperature, the Na layers in Na0.8CoO2 adopt a 2D liquid state at T=291 K. The corresponding first order phase transition is preceded by a rapidly increasing mobility and diffusion of Na ions above 200 K. Above 291 K, the 23Na NMR response is similar to that previously observed in superionic conductors with planar Na layers. PMID:19257527

Weller, M; Sacchetti, A; Ott, H R; Mattenberger, K; Batlogg, B

2009-02-01

107

Hypernatremia inhibits NaHCO3 reabsorption and associated NaCl reabsorption in dogs  

Microsoft Academic Search

Hypernatremia inhibits NaHCO3 reabsorption and associated NaCl reabsorption in dogs. To examine the effect of selective rise of plasma NaCl concentration (hypematremia) on NaHCO3 reabsorption and associated NaCl reabsorption remaining during continuous ethacrynic acid infusion, hypertonic NaCl solution was infused in three groups of anesthetized volume-expanded dogs. In six dogs examined at constant hematocrit and plasma pH, bicarbonate and water

Harald Langberg; Anders Hartmann; Jonny Østensen; Øystein Mathisen; Fredrik Kiil

1986-01-01

108

Ionic regulation of Na absorption in proximal colon: cation inhibition of electroneutral Na absorption  

SciTech Connect

Active Na absorption (J/sub net//sup NA/) in rabbit proximal colon in vitro is paradoxically stimulated as (Na) in the bathing media is lowered with constant osmolarity. J/sub m..-->..s//sup Na/ increases almost linearly from 0 to 50 mM (Na)/sub 0/ but then plateaus and actually decreases from 50 to 140 mM (Na)/sub 0/, consistent with inhibition of an active transport process. Both lithium and Na are equally effective inhibitors of J/sub net//sup Na/, whereas choline and mannitol do not block the high rate of J/sub net//sup Na/ observed in decreased (Na)/sub 0/. Either gluconate or proprionate replacement of Cl inhibits J/sub net//sup Na/. J/sub net//sup Na/ at lowered (Na)/sub 0/ is electrically silent and is accompanied by increased Cl absorption; it is inhibited by 10/sup -3/ M amiloride and 10/sup -3/ theophylline but not by 10/sup -4/ M bumetanide. Epinephrine is equally effective at stimulating Na absorption at 50 and 140 mM (Na). Na gradient experiments are consistent with a predominantly serosal effect of the decreased (Na)/sub 0/. These results suggest that 1) Na absorption in rabbit proximal colon in vitro is stimulated by decreased (Na); 2) the effect is cation specific, both Na and Li blocking the stimulatory effect; 3) the transport is mediated by Na-H exchange and is Cl dependent but 4) is under different regulatory mechanisms than the epinephrine-sensitive Na-Cl cotransport previously described in proximal colon. Under the appropriate conditions, proximal colon absorbs Na extremely efficiently. Na-H exchange in this epithelium is cation inhibitable, either directly or by a secondary regulatory process.

Sellin, J.H.; De Soignie, R.

1987-01-01

109

Na+ Tolerance and Na+ Transport in Higher Plants  

PubMed Central

Tolerance to high soil [Na+] involves processes in many different parts of the plant, and is manifested in a wide range of specializations at disparate levels of organization, such as gross morphology, membrane transport, biochemistry and gene transcription. Multiple adaptations to high [Na+] operate concurrently within a particular plant, and mechanisms of tolerance show large taxonomic variation. These mechanisms can occur in all cells within the plant, or can occur in specific cell types, reflecting adaptations at two major levels of organization: those that confer tolerance to individual cells, and those that contribute to tolerance not of cells per se, but of the whole plant. Salt?tolerant cells can contribute to salt tolerance of plants; but we suggest that equally important in a wide range of conditions are processes involving the management of Na+ movements within the plant. These require specific cell types in specific locations within the plant catalysing transport in a coordinated manner. For further understanding of whole plant tolerance, we require more knowledge of cell?specific transport processes and the consequences of manipulation of transporters and signalling elements in specific cell types. PMID:12646496

TESTER, MARK; DAVENPORT, ROMOLA

2003-01-01

110

Na+-stimulated ATPase of alkaliphilic halotolerant cyanobacterium Aphanothece halophytica translocates Na+ into proteoliposomes via Na+ uniport mechanism  

PubMed Central

Background When cells are exposed to high salinity conditions, they develop a mechanism to extrude excess Na+ from cells to maintain the cytoplasmic Na+ concentration. Until now, the ATPase involved in Na+ transport in cyanobacteria has not been characterized. Here, the characterization of ATPase and its role in Na+ transport of alkaliphilic halotolerant Aphanothece halophytica were investigated to understand the survival mechanism of A. halophytica under high salinity conditions. Results The purified enzyme catalyzed the hydrolysis of ATP in the presence of Na+ but not K+, Li+ and Ca2+. The apparent Km values for Na+ and ATP were 2.0 and 1.2 mM, respectively. The enzyme is likely the F1F0-ATPase based on the usual subunit pattern and the protection against N,N'-dicyclohexylcarbodiimide inhibition of ATPase activity by Na+ in a pH-dependent manner. Proteoliposomes reconstituted with the purified enzyme could take up Na+ upon the addition of ATP. The apparent Km values for this uptake were 3.3 and 0.5 mM for Na+ and ATP, respectively. The mechanism of Na+ transport mediated by Na+-stimulated ATPase in A. halophytica was revealed. Using acridine orange as a probe, alkalization of the lumen of proteoliposomes reconstituted with Na+-stimulated ATPase was observed upon the addition of ATP with Na+ but not with K+, Li+ and Ca2+. The Na+- and ATP-dependent alkalization of the proteoliposome lumen was stimulated by carbonyl cyanide m - chlorophenylhydrazone (CCCP) but was inhibited by a permeant anion nitrate. The proteoliposomes showed both ATPase activity and ATP-dependent Na+ uptake activity. The uptake of Na+ was enhanced by CCCP and nitrate. On the other hand, both CCCP and nitrate were shown to dissipate the preformed electric potential generated by Na+-stimulated ATPase of the proteoliposomes. Conclusion The data demonstrate that Na+-stimulated ATPase from A. halophytica, a likely member of F-type ATPase, functions as an electrogenic Na+ pump which transports only Na+ upon hydrolysis of ATP. A secondary event, Na+- and ATP-dependent H+ efflux from proteoliposomes, is driven by the electric potential generated by Na+-stimulated ATPase. PMID:20691102

2010-01-01

111

Phase transitions and equations of state for the sodium halides: NaF NaCl, NaBr, and NaI  

NASA Astrophysics Data System (ADS)

Phase changes and equations of state of four sodium halides, NaF, NaCl, NaBr, and NaI, have been studied up to ˜60 GPa at room temperature with the use of a diamond anvil cell. NaF and NaCl transform from the B1 phase to the B2 phase at ˜23 GPa and ˜29 GPa, respectively. At ˜33 GPa, NaBr transforms from the B1 phase to an unidentified phase with a lower symmetry than the B2 phase. NaI also transforms at ˜32 GPa to a new phase which seems to have the same structure as that of the high-pressure phase of NaBr. The B2 phase of NaF has bulk modulus K0 = 103 (±19) GPa and volume ratio at 1 atm Vo2/Vo1 = 0.811 (±0.025), and the B2 phase of NaCl has K0 = 121 (±23) GPa and V02/V01 = 0.734 (±0.022). The volume change across the transition was estimated to be ?V/V1 ? -10.4% for NaF and -5.8% for NaCl.

Sato-Sorensen, Yosiko

1983-04-01

112

NA  

E-print Network

Information regarding the chemical identity of benzene is located in Table 4-1. Although the term benzol is found in older literature for the commercial product, benzene is the name presently approved by the International Union of Pure and Applied Chemistry (IUPAC), the Chemical Manufacturers Association (CMA), and the American Society for Testing and Materials (ASTM) for the pure product.

unknown authors

113

Aithisg Bhliadhnail Coilltearachd na h-Alba  

E-print Network

Aithisg Bhliadhnail �ghdarras Coilltearachd na h-Alba Lèirmheas Bliadhnail 2007-2008 #12;�ghdarras Coilltearachd na h-Alba Lèirmheas Bliadhnail 2007-08 1 | �ghdarras Coilltearachd na h-Alba Lèirmheas Bliadhnail Leasachadh coimhearsnachd 11 Ruigsinneachd agus slàinte 14 Càileachd na h-àrainneachd 17 Bith-iomadachd 19 Mu

114

Photoabsorption windows for Na and Na{sub 2}{sup +}  

SciTech Connect

There exist absorption windows (i.e., Cooper-minima) in the photoabsorption cross sections of some atomic systems because of the relative phase shifts between the initial and final state wave functions due to the atomic screening potentials in contrast to the Coulomb potentials. Such window positions are sensitive to the accuracy of the initial and final state wave functions. Using our modified Breit-Pauli R-matrix code, the photoionization cross sections of ground Na are calculated. Our calculated cross sections and minimum position in the low photoelectron energy range (<9 eV) are in excellent agreement with the experimental results. In the high energy range (>9 eV), there is an abnormal bump in the experimental measurements, which is a long-standing experimental puzzle. It is interesting to note that there is also an absorption window in the photoabsorption (i.e., photodissociation) cross sections of Na{sub 2}{sup +}. Such an absorption window provides an answer to the puzzle.

Han Xiaoying [Key Laboratory of Atomic and Molecular Nanosciences of Education Ministry, Department of Physics, Tsinghua University, Beijing 100084 (China); Gao Xiang [Department of Physics, Shanghai Jiao Tong University, Shanghai 200230 (China); Li Jiaming [Department of Physics, Shanghai Jiao Tong University, Shanghai 200230 (China); Key Laboratory of Atomic and Molecular Nanosciences of Education Ministry, Department of Physics, Tsinghua University, Beijing 100084 (China); Voky, Lan; Feautrier, Nicole [LERMA, UMR 8112 du CNRS, Observatoire de Paris, 92915 Meudon Cedex (France)

2006-12-15

115

Na + ,K + ATPase Na + Affinity in Rat Skeletal Muscle Fiber Types  

Microsoft Academic Search

Previous studies in expression systems have found different ion activation of the Na+\\/K+-ATPase isozymes, which suggest that different muscles have different ion affinities. The rate of ATP hydrolysis was used\\u000a to quantify Na+,K+-ATPase activity, and the Na+ affinity of Na+,K+-ATPase was studied in total membranes from rat muscle and purified membranes from muscle with different fiber types. The\\u000a Na+ affinity

Michael Kristensen; Carsten Juel

2010-01-01

116

Uloga polimorfizama i ekspresije gena za hemokine CX3C ligand 1 i CXC ligand 16 i njihove receptore u nastanku i progresiji multiple skleroze u Srbiji.  

E-print Network

??Molekularna biomedicina - Molekularna genetika multiple skleroze / Molecular - Molecular genetics of multiple sclerosis Multipla skleroza je hroni?na inflamatorna, autoimunska, demijelinizaciona i neurodegenerativna bolest… (more)

Stojkovi?, Ljiljana S., 1979-

2014-01-01

117

Reviews  

E-print Network

Savinjski dolini, na Kozjanskem, na Ptujskem polju, v Prlekiji in Prekmurju delalo 410 lon?arjev, leta 1993 pa jih je bilo le 20. Nare?no strokovno lon?arsko izrazje je dvojno: delno gre za posebno, enopo- mensko besedje, ki se nanaša le na pred- mete... antologijah. Gre za sistemati?no raz- ?lembo in interpretacijo pesni?inega je- zikovnega izraza, kar je glede na avtor- jevo siceršnjo raziskovalno usmerjenost in dejavnost univerzitetnega profesorja za moderni nemški jezik in stilistiko pri?akovano. Prof...

2009-02-01

118

Relationship between intracellular Na+ concentration and reduced Na+ affinity in Na+,K+-ATPase mutants causing neurological disease.  

PubMed

The neurological disorders familial hemiplegic migraine type 2 (FHM2), alternating hemiplegia of childhood (AHC), and rapid-onset dystonia parkinsonism (RDP) are caused by mutations of Na(+),K(+)-ATPase ?2 and ?3 isoforms, expressed in glial and neuronal cells, respectively. Although these disorders are distinct, they overlap in phenotypical presentation. Two Na(+),K(+)-ATPase mutations, extending the C terminus by either 28 residues ("+28" mutation) or an extra tyrosine ("+Y"), are associated with FHM2 and RDP, respectively. We describe here functional consequences of these and other neurological disease mutations as well as an extension of the C terminus only by a single alanine. The dependence of the mutational effects on the specific ? isoform in which the mutation is introduced was furthermore studied. At the cellular level we have characterized the C-terminal extension mutants and other mutants, addressing the question to what extent they cause a change of the intracellular Na(+) and K(+) concentrations ([Na(+)]i and [K(+)]i) in COS cells. C-terminal extension mutants generally showed dramatically reduced Na(+) affinity without disturbance of K(+) binding, as did other RDP mutants. No phosphorylation from ATP was observed for the +28 mutation of ?2 despite a high expression level. A significant rise of [Na(+)]i and reduction of [K(+)]i was detected in cells expressing mutants with reduced Na(+) affinity and did not require a concomitant reduction of the maximal catalytic turnover rate or expression level. Moreover, two mutations that increase Na(+) affinity were found to reduce [Na(+)]i. It is concluded that the Na(+) affinity of the Na(+),K(+)-ATPase is an important determinant of [Na(+)]i. PMID:24356962

Toustrup-Jensen, Mads S; Einholm, Anja P; Schack, Vivien R; Nielsen, Hang N; Holm, Rikke; Sobrido, María-Jesús; Andersen, Jens P; Clausen, Torben; Vilsen, Bente

2014-02-01

119

Growth of binary organic NLO crystals: m.NA-p.NA and m.NA-CNA system  

NASA Technical Reports Server (NTRS)

Experiments were carried out to grow 3.Nitroaniline (m.NA) crystals doped with 4.Nitroaniline (p.NA) and 2.chloro 4.Nitroaniline (CNA). The measured undercooling for m.NA, p.NA, and CNA were 0.21 tm K, 0.23 tm K, and 0.35 tm K respectively, where tm represents the melting temperature of the pure component. Because of the crystals' large heat of fusion and large undercooling, it was not possible to grow good quality crystals with low thermal gradients. In the conventional two-zone Bridgman furnace we had to raise the temperature of the hot zone above the decomposition temperature of CNA, p.NA, and m.NA to achieve the desired thermal gradient. To avoid decomposition, we used an unconventional Bridgman furnace. Two immiscible liquids, silicone oil and ethylene glycol, were used to build a special two-zone Bridgman furnace. A temperature gradient of 18 K/cm was achieved without exceeding the decomposition temperature of the crystal. The binary crystals, m.NA-p.NA and m.NA-CNA, were grown in centimeter size in this furnace. X-ray and optical characterization showed good optical quality.

Singh, N. B.; Henningsen, T.; Hopkins, R. H.; Mazelsky, R.

1993-01-01

120

Evaluation of Fc?RIIIB-NA1/NA2 Polymorphism in Visceral Leishmaniasis  

PubMed Central

Background: Several lines of evidence demonstrating that innate and adaptive immunity play important roles in the defense against visceral leishmaniasis (VL). A polymorphism within the Fc?RIIIB gene can lead to the expression of three variants of NA1, NA2, and the combined one (NA1/NA2) which alters affinity of IgG to its receptor. Objectives: The main aim of this study was to evaluate the Fc?RIIIB-NA1/NA2 polymorphism in the Fc?RIIIB gene of VL patients in comparison to healthy controls. Patients and Methods: In this cross-sectional study, three groups; 54 seropositive patients with clinical presentation of VL (group 1), 104 seropositive patients without clinical presentation (group 2), and 104 healthy controls (group 3) were evaluated with respect to the Fc?RIIIB-NA1/NA2 polymorphism using a PCR-SSP method. The titration of anti-leishmania antibodies was analyzed using an immunoflorescence technique. Results: Our results indicated that polymorphisms within the Fc?RIIIB gene (that lead to the expression of the NA1/NA2 isoforms) are significantly associated with VL. The results demonstrated that the genotype heterozygotic for Fc?RIIIB-NA1/NA2 expression was significantly increased in VL patients, group 1 when compared to groups 2 and 3. Conversely, there is a decrease in homozygous NA1 and NA2 genotypes in VL patients; however, the overall frequency of NA1 and NA2 alleles appear similar across the three cohorts examined. Conclusions: According to our results, it is likely that the increased frequency of the Fc?RIIIB-NA1/NA2 genotype is associated with impaired immune responses against VL and its subsequent clearance from the patient. PMID:24910789

Abasi, Mohammad; Lotfi, Pegah; Bazmani, Ahad; Matini, Mohamad; Hajilooi, Mehrdad

2014-01-01

121

UltraHigh NA Structured Illumination Microscopy  

Microsoft Academic Search

We measure the three-dimensional point-spread function and compare the resolution of three high numerical aperture objectives (NA) in a structured illumination fluorescence microscope using three-dimensional images of single quantum dots. Compared with conventional 1.30 and 1.42 NA objectives, the ultra-high 1.65 NA TIRF objectives offer significantly improved lateral spatial resolution of better than 75 nm using 488 nm excitation.

Michael Beversluis; Stephan Stranick

2007-01-01

122

Modeling Na clusters in Ar matrices  

Microsoft Academic Search

We present a microscopic model for Na clusters embedded in raregas matrices. The valence electrons of the Na cluster are described by time-dependent density-functional theory at the level of the local-density approximation (LDA). Particular attention is paid to the semi-classical picture in terms of Vlasov-LDA. The Na+ ions and Ar atoms are handled as classical particles whereby the Ar atoms

F. Fehrer; M. Mundt; P.-G. Reinhard; E. Suraud

2005-01-01

123

Painful Na-channelopathies: an expanding universe.  

PubMed

The universe of painful Na-channelopathies--human disorders caused by mutations in voltage-gated sodium channels--has recently expanded in three dimensions. We now know that mutations of sodium channels cause not only rare genetic 'model disorders' such as inherited erythromelalgia and channelopathy-associated insensitivity to pain but also common painful neuropathies. We have learned that mutations of NaV1.8, as well as mutations of NaV1.7, can cause painful Na-channelopathies. Moreover, recent studies combining atomic level structural models and pharmacogenomics suggest that the goal of genomically guided pain therapy may not be unrealistic. PMID:23664154

Waxman, Stephen G

2013-07-01

124

Cerebral Na concentration, Na appetite and thirst of sheep: influence of somatostatin and losartan.  

PubMed

Na and water intakes of Na-depleted sheep are influenced by changes in cerebral Na concentration. The effect of intracerebroventricular infusion of somatostatin or losartan, the ANG II type 1 receptor antagonist, on the Na appetite and thirst of Na-depleted sheep during infusions that decrease (intracerebroventricular hypertonic mannitol) or increase (intracerebroventricular or systemic hypertonic NaCl) cerebral Na concentration was investigated. Na intake was increased but water intake was unchanged during intracerebroventricular infusion of hypertonic mannitol. The increased Na appetite caused by intracerebroventricular infusion of hypertonic mannitol was decreased by concurrent intracerebroventricular infusion of either somatostatin or losartan, with somatostatin being most effective. Water intake was increased during intracerebroventricular infusion of hypertonic mannitol and somatostatin. Na intake was decreased and water intake was increased during systemic or intracerebroventricular infusion of hypertonic NaCl. Intracerebroventricular infusion of losartan blocked both (Na and water intake), whereas somatostatin did not influence either of these changes in intake. The results further consolidate a role for somatostatin and ANG II in the central mechanisms controlling Na appetite and thirst of sheep. PMID:11171646

Weisinger, R S; Blair-West, J R; Burns, P; Denton, D A; Purcell, B

2001-03-01

125

Epithelial Na(+) channels are regulated by flow.  

PubMed

Na(+) absorption in the renal cortical collecting duct (CCD) is mediated by apical epithelial Na(+) channels (ENaCs). The CCD is subject to continuous variations in intraluminal flow rate that we speculate alters hydrostatic pressure, membrane stretch, and shear stress. Although ENaCs share limited sequence homology with putative mechanosensitive ion channels in Caenorhabditis elegans, controversy exists as to whether ENaCs are regulated by biomechanical forces. We examined the effect of varying the rate of fluid flow on whole cell Na(+) currents (I(Na)) in oocytes expressing mouse alpha,beta,gamma-ENaC (mENaC) and on net Na(+) absorption in microperfused rabbit CCDs. Oocytes injected with mENaC but not water responded to the initiation of superfusate flow (to 4-6 ml/min) with a reversible threefold stimulation of I(Na) without a change in reversal potential. The increase in I(Na) was variable among oocytes. CCDs responded to a threefold increase in rate of luminal flow with a twofold increase in the rate of net Na(+) absorption. An increase in luminal viscosity achieved by addition of 5% dextran to the luminal perfusate did not alter the rate of net Na(+) absorption, suggesting that shear stress does not influence Na(+) transport in the CCD. In sum, our data suggest that flow stimulation of ENaC activity and Na(+) absorption is mediated by an increase in hydrostatic pressure and/or membrane stretch. We propose that intraluminal flow rate may be an important regulator of channel activity in the CCD. PMID:11352841

Satlin, L M; Sheng, S; Woda, C B; Kleyman, T R

2001-06-01

126

Interfering Rydberg wave packets in Na  

Microsoft Academic Search

This paper presents an experimental and theoretical analysis of quantum interference between Rydberg wave packets in Na. Pairs of phase-locked wave packets manipulate the total orbital angular momentum of Na Rydberg atoms. Initially, the wave packet is composed of a superposition of s and d Rydberg series. Exploitation of the difference between the quantum defects of the two series allows

R. E. Carley; E. D. Boléat; R. S. Minns; R. Patel; H. H. Fielding

2005-01-01

127

NEODZIVNOST NA PERORALNO ANTIAGREGACIJSKO ZDRAVLJENJE NON-RESPONSIVENESS TO ORAL ANTIPLATELET TREATMENT  

Microsoft Academic Search

Klju?ne besede antiagregacijsko zdravljenje; neodzivnost; aspirin; klopidogrel Izvle?ek Izhodiš?a Antiagregacijsko zdravljenje z aspirinom in\\/ali klopidogrelom zmanjšuje pogostost aterotromboti?nih zapletov, ne prepre?i pa jih v celoti. Pri multifaktorskem bolezenskem procesu, kot je aterotromboza, ni mogo?e pri?akovati, da bi bila ena sama vrsta zdravljen- ja povsem u?inkovita, vendar zbuja skrb dejstvo, da v klini?ni praksi predpisujemo anti- agregacijska zdravila brez merjenja njihovih

Rok Perme

128

Regulation of Na+ fluxes in plants  

PubMed Central

When exposed to salt, every plant takes up Na+ from the environment. Once in the symplast, Na+ is distributed within cells and between different tissues and organs. There it can help to lower the cellular water potential but also exert potentially toxic effects. Control of Na+ fluxes is therefore crucial and indeed, research shows that the divergence between salt tolerant and salt sensitive plants is not due to a variation in transporter types but rather originates in the control of uptake and internal Na+ fluxes. A number of regulatory mechanisms has been identified based on signaling of Ca2+, cyclic nucleotides, reactive oxygen species, hormones, or on transcriptional and post translational changes of gene and protein expression. This review will give an overview of intra- and intercellular movement of Na+ in plants and will summarize our current ideas of how these fluxes are controlled and regulated in the early stages of salt stress.

Maathuis, Frans J. M.; Ahmad, Izhar; Patishtan, Juan

2014-01-01

129

Substrate interactions of the electroneutral Na+-coupled inorganic phosphate cotransporter (NaPi-IIc)  

PubMed Central

The SLC34 solute carrier family comprises the electrogenic NaPi-IIa/b and the electroneutral NaPi-IIc, which display Na+ : Pi cotransport stoichiometries of 3 : 1 and 2 : 1, respectively. We previously proposed that NaPi-IIc lacks one of the three Na+ interaction sites hypothesised for the electrogenic isoforms, but, unlike NaPi-IIa/b, its substrate binding order is undetermined. By expressing NaPi-IIc in Xenopus oocytes, isotope influx and efflux assays gave results consistent with Na+ being the first and last substrate to bind. To further investigate substrate interactions, we applied a fluorometry-based technique that uses site-specific labelling with a fluorophore to characterize substrate-induced conformational changes. A novel Cys was introduced in the third extracellular loop of NaPi-IIc that could be labelled with a reporter fluorophore (MTS-TAMRA). Although labelling resulted in suppression of cotransport as previously reported for the electrogenic isoforms, changes in fluorescence were induced by changes in extracellular Na+ concentration in the absence of Pi and by changes in extracellular Pi concentration in presence of Na+. These data, combined with 32P uptake data, also support a binding scheme in which Na+ is the first substrate to interact. Moreover, the apparent Pi affinity from fluorometry agreed with that from 32P uptake, confirming the applicability of the fluorometric technique for kinetic studies of electroneutral carriers. Analysis of the fluorescence data showed that like the electrogenic NaPi-IIb, 2 Na+ ions interact cooperatively with NaPi-IIc before Pi binding, which implies that only one of these is translocated. This result provides compelling evidence that SLC34 proteins share common motifs for substrate interaction and that cotransport and substrate binding stoichiometries are not necessarily equivalent. PMID:19596895

Ghezzi, Chiara; Murer, Heini; Forster, Ian C

2009-01-01

130

On the formation of transient (Na19)2 and (Na20)2 cluster dimers from molecular dynamics simulations  

Microsoft Academic Search

By using distance-dependent tight-binding molecular dynamics simulations, we discuss the possibilities to form (Na19)2 and (Na20)2 cluster dimers in sodium cluster-cluster collisions. In the case of Na19+Na19, we show that the formation of a prolate dimer-like (Na19)2 may depend on the initial relative orientations of the colliding clusters. A similar study for Na20+Na20 does not seen to show the same

F. S. Zhang; F. Spiegelmann; E. Suraud; V. Frayssé; R. Poteau; R. Glowinski; F. Chatelin

1994-01-01

131

Polarization dependence of Na/emph>+Na/emph> associative ionization revisited  

NASA Astrophysics Data System (ADS)

The dependence of the associative ionization process Na 3 2P3/2+Na 3 2P3/2-->Na2 ++e- on the polarization of the laser light used for Na excitation was independently investigated in Utrecht and Berlin. The purpose of this paper is to clarify discrepancies between earlier experimental results of Kircz, Morgenstern, and Nienhuis, on one hand, and Rothe, Theyunni, Reck, and Tung on the other hand. The new results confirm in general the data of Kircz, Morgenstern, and Nienhuis, and also indicate a dependence of the anisotropy ratios on the relative velocity of the interacting Na* atoms.

Meijer, H. A. J.; Meulen, H. P. V. D.; Morgenstern, R.; Hertel, I. V.; Meyer, E.; Schmidt, H.; Witte, R.

1986-02-01

132

Race and sex differences in erythrocyte Na+, K+, and Na+-K+-adenosine triphosphatase.  

PubMed Central

Several reports indicate that erythrocytes (RBCs) from blacks and men have higher sodium concentrations than those from whites and women. One possible mechanism to explain this finding is a difference in the activity of Na+-K+-ATPase. To explore this possibility, we have studied the Na+ and K+ kinetics of RBC Na+-K+-ATPase and RBC Na+ and K+ concentrations in 37 normotensive blacks and whites, both males and females. The maximal initial reaction velocity (Vmax) values for RBC Na+-K+-ATPase were lower in blacks and men as compared with whites and women. Higher RBC Na+ levels were observed in blacks and males vs. whites and females. Significant inverse correlations were noted between the Na+-K+-ATPase activity and RBC Na+ concentrations. These findings indicate that cellular Na+ homeostasis is different in blacks and men as compared with whites and women. Since higher RBC Na+ concentrations have also been observed in patients with essential hypertension as compared with normotensive subjects, the higher intracellular Na+ concentrations in blacks and men may contribute to the greater predisposition of these groups to essential hypertension. PMID:2989333

Lasker, N; Hopp, L; Grossman, S; Bamforth, R; Aviv, A

1985-01-01

133

Cytosolic Na+ Controls an Epithelial Na+ Channel Via the Go Guanine Nucleotide-Binding Regulatory Protein  

NASA Astrophysics Data System (ADS)

In tight Na+-absorbing epithelial cells, the rate of Na+ entry through amiloride-sensitive apical membrane Na+ channels is matched to basolateral Na+ extrusion so that cell Na+ concentration and volume remain steady. Control of this process by regulation of apical Na+ channels has been attributed to changes in cytosolic Ca2+ concentration or pH, secondary to changes in cytosolic Na+ concentration, although cytosolic Cl- seems also to be involved. Using mouse mandibular gland duct cells, we now demonstrate that increasing cytosolic Na+ concentration inhibits apical Na+ channels independent of changes in cytosolic Ca2+, pH, or Cl-, and the effect is blocked by GDP-? -S, pertussis toxin, and antibodies against the ? -subunits of guanine nucleotide-binding regulatory proteins (Go). In contrast, the inhibitory effect of cytosolic anions is blocked by antibodies to inhibitory guanine nucleotide-binding regulatory proteins (Gi1/Gi2. It thus appears that apical Na+ channels are regulated by Go and Gi proteins, the activities of which are controlled, respectively, by cytosolic Na+ and Cl-.

Komwatana, P.; Dinudom, A.; Young, J. A.; Cook, D. I.

1996-07-01

134

Gasotransmitters: Novel Regulators of Epithelial Na+ Transport?  

PubMed Central

The vectorial transport of Na+ across epithelia is crucial for the maintenance of Na+ and water homeostasis in organs such as the kidneys, lung, or intestine. Dysregulated Na+ transport processes are associated with various human diseases such as hypertension, the salt-wasting syndrome pseudohypoaldosteronism type 1, pulmonary edema, cystic fibrosis, or intestinal disorders, which indicate that a precise regulation of epithelial Na+ transport is essential. Novel regulatory signaling molecules are gasotransmitters. There are currently three known gasotransmitters: nitric oxide (NO), carbon monoxide (CO), and hydrogen sulfide (H2S). These molecules are endogenously produced in mammalian cells by specific enzymes and have been shown to regulate various physiological processes. There is a growing body of evidence which indicates that gasotransmitters may also regulate Na+ transport across epithelia. This review will summarize the available data concerning NO, CO, and H2S dependent regulation of epithelial Na+ transport processes and will discuss whether or not these mediators can be considered as true physiological regulators of epithelial Na+ transport biology. PMID:22509167

Althaus, Mike

2012-01-01

135

Gasotransmitters: novel regulators of epithelial na(+) transport?  

PubMed

The vectorial transport of Na(+) across epithelia is crucial for the maintenance of Na(+) and water homeostasis in organs such as the kidneys, lung, or intestine. Dysregulated Na(+) transport processes are associated with various human diseases such as hypertension, the salt-wasting syndrome pseudohypoaldosteronism type 1, pulmonary edema, cystic fibrosis, or intestinal disorders, which indicate that a precise regulation of epithelial Na(+) transport is essential. Novel regulatory signaling molecules are gasotransmitters. There are currently three known gasotransmitters: nitric oxide (NO), carbon monoxide (CO), and hydrogen sulfide (H(2)S). These molecules are endogenously produced in mammalian cells by specific enzymes and have been shown to regulate various physiological processes. There is a growing body of evidence which indicates that gasotransmitters may also regulate Na(+) transport across epithelia. This review will summarize the available data concerning NO, CO, and H(2)S dependent regulation of epithelial Na(+) transport processes and will discuss whether or not these mediators can be considered as true physiological regulators of epithelial Na(+) transport biology. PMID:22509167

Althaus, Mike

2012-01-01

136

The voltage-gated Na channel NaVBP has a role in motility, chemotaxis, and pH homeostasis  

E-print Network

The voltage-gated Na channel NaVBP has a role in motility, chemotaxis, and pH homeostasis, is a voltage-gated Na channel potentiated by alkaline pH. NaVBP has roles in motility, chemotaxis, and pH homeostasis at high pH. Reduced motility of bacteria lacking functional NaVBP was reversed by restoration

Clapham, David E.

137

Catalysis of Na+ permeation in the bacterial sodium channel Na(V)Ab.  

PubMed

Determination of a high-resolution 3D structure of voltage-gated sodium channel Na(V)Ab opens the way to elucidating the mechanism of ion conductance and selectivity. To examine permeation of Na(+) through the selectivity filter of the channel, we performed large-scale molecular dynamics simulations of Na(V)Ab in an explicit, hydrated lipid bilayer at 0 mV in 150 mM NaCl, for a total simulation time of 21.6 ?s. Although the cytoplasmic end of the pore is closed, reversible influx and efflux of Na(+) through the selectivity filter occurred spontaneously during simulations, leading to equilibrium movement of Na(+) between the extracellular medium and the central cavity of the channel. Analysis of Na(+) dynamics reveals a knock-on mechanism of ion permeation characterized by alternating occupancy of the channel by 2 and 3 Na(+) ions, with a computed rate of translocation of (6 ± 1) × 10(6) ions?s(-1) that is consistent with expectations from electrophysiological studies. The binding of Na(+) is intimately coupled to conformational isomerization of the four E177 side chains lining the extracellular end of the selectivity filter. The reciprocal coordination of variable numbers of Na(+) ions and carboxylate groups leads to their condensation into ionic clusters of variable charge and spatial arrangement. Structural fluctuations of these ionic clusters result in a myriad of ion binding modes and foster a highly degenerate, liquid-like energy landscape propitious to Na(+) diffusion. By stabilizing multiple ionic occupancy states while helping Na(+) ions diffuse within the selectivity filter, the conformational flexibility of E177 side chains underpins the knock-on mechanism of Na(+) permeation. PMID:23803856

Chakrabarti, Nilmadhab; Ing, Christopher; Payandeh, Jian; Zheng, Ning; Catterall, William A; Pomès, Régis

2013-07-01

138

Catalysis of Na+ permeation in the bacterial sodium channel NaVAb  

PubMed Central

Determination of a high-resolution 3D structure of voltage-gated sodium channel NaVAb opens the way to elucidating the mechanism of ion conductance and selectivity. To examine permeation of Na+ through the selectivity filter of the channel, we performed large-scale molecular dynamics simulations of NaVAb in an explicit, hydrated lipid bilayer at 0 mV in 150 mM NaCl, for a total simulation time of 21.6 ?s. Although the cytoplasmic end of the pore is closed, reversible influx and efflux of Na+ through the selectivity filter occurred spontaneously during simulations, leading to equilibrium movement of Na+ between the extracellular medium and the central cavity of the channel. Analysis of Na+ dynamics reveals a knock-on mechanism of ion permeation characterized by alternating occupancy of the channel by 2 and 3 Na+ ions, with a computed rate of translocation of (6 ± 1) × 106 ions?s?1 that is consistent with expectations from electrophysiological studies. The binding of Na+ is intimately coupled to conformational isomerization of the four E177 side chains lining the extracellular end of the selectivity filter. The reciprocal coordination of variable numbers of Na+ ions and carboxylate groups leads to their condensation into ionic clusters of variable charge and spatial arrangement. Structural fluctuations of these ionic clusters result in a myriad of ion binding modes and foster a highly degenerate, liquid-like energy landscape propitious to Na+ diffusion. By stabilizing multiple ionic occupancy states while helping Na+ ions diffuse within the selectivity filter, the conformational flexibility of E177 side chains underpins the knock-on mechanism of Na+ permeation. PMID:23803856

Chakrabarti, Nilmadhab; Ing, Christopher; Payandeh, Jian; Zheng, Ning; Catterall, William A.; Pomes, Regis

2013-01-01

139

Rastafari : identidade e hibridismo cultural na Jamaica, 1930-1981.  

E-print Network

??Tese(doutorado)—Universidade de Brasília/Departamento de História, 2006. Na Jamaica, o passado colonial baseado na plantation escravista, produziu uma sociedade estratificada na qual a cor mais clara… (more)

Rabelo, Danilo

2006-01-01

140

An Update on NA50 and LUCIFER  

E-print Network

Recent analysis of both new (1998) and old (1995,1996) data obtained by the NA50 Collaboration on J/Psi suppression in Pb+Pb are examined in light of our already existing calculations with the relativistic heavy ion simulation LUCIFER. In particular we comment on the unexplained change of transverse energy scale by NA50 and the apparent disappearance from the data of discontinuities in the suppression with transverse energy.

D. E. Kahana; S. H. Kahana

1999-08-20

141

An Update on NA50 and LUCIFER  

E-print Network

Recent analysis of both new (1998) and old (1995,1996) data obtained by the NA50 Collaboration on J/Psi suppression in Pb+Pb are examined in light of our already existing calculations with the relativistic heavy ion simulation LUCIFER. In particular we comment on the unexplained change of transverse energy scale by NA50 and the apparent disappearance from the data of discontinuities in the suppression with transverse energy.

Kahana, D E

1999-01-01

142

Electrogenic Na +\\/Ca 2+-exchange of nerve and muscle cells  

Microsoft Academic Search

The plasma membrane Na+\\/Ca2+-exchanger is a bi-directional electrogenic (3Na+:1Ca2+) and voltage-sensitive ion transport mechanism, which is mainly responsible for Ca2+-extrusion. The Na+-gradient, required for normal mode operation, is created by the Na+-pump, which is also electrogenic (3Na+:2K+) and voltage-sensitive. The Na+\\/Ca2+-exchanger operational modes are very similar to those of the Na+-pump, except that the uncoupled flux (Na+-influx or -efflux?) is

Tamás L. Török

2007-01-01

143

Vibrational signatures of sodiated oligopeptides (GG-Na+, GGG-Na+, AA-Na+ and AAA-Na+) in the gas phase  

NASA Astrophysics Data System (ADS)

The structures of the sodium complexes of oligoglycines (GG-Na+, GGG-Na+) and oligoalanines (AA-Na+, AAA-Na+) have been studied by infrared spectroscopy in the gas phase. Two different experimental set-ups have been used to generate, trap and analyze the ions. In the first, the complexes were generated by MALDI and analyzed in the cell of a home built FT-ICR mass spectrometer. In the second an external electrospray source was coupled to a Paul type ion trap. Following their trapping, the ions are irradiated in both cases with intense, tunable infrared light in the 1000-2000 cm-1 range, leading to sodium ion detachment and ion fragmentation via the absorption of multiple photons. The resulting experimental spectra are compared to theoretical linear absorption spectra to assign structures. In agreement with calculations, peptide attachment to Na+ is found to have a strong structuring effect: the lowest energy structures involve binding of all carbonyl oxygens to the cation. Detailed comparison of experimental and computed spectra shows that the IRMPD spectroscopy of such gaseous ions allows the differentiation between structures which do not have the same number of carbonyl oxygens bound to Na+, and structures in which the peptide is either wrapped around the ion or capped by it.

Balaj, O. P.; Kapota, C.; Lemaire, J.; Ohanessian, G.

2008-02-01

144

Modeling Na clusters in Ar matrices  

E-print Network

We present a microscopic model for Na clusters embedded in raregas matrices. The valence electrons of the Na cluster are described by time-dependent density-functional theory at the level of the local-density approximation (LDA). Particular attention is paid to the semi-classical picture in terms of Vlasov-LDA. The Na ions and Argon atoms are handled as classical particles whereby the Ar atoms carry two degrees of freedom, position and dipole polarization. The interaction between Na ions and electrons is mediated through local pseudo-potentials. The coupling to the Ar atoms is described by (long-range) polarization potentials and (short-range) repulsive cores. The ingredients are taken from elsewhere developed standards. A final fine-tuning is performed using the NaAr molecule as benchmark. The model is then applied to embedded systems Na8ArN. By close comparison with quantum-mechanical results, we explore the capability of the Vlasov-LDA to describe such embedded clusters. We show that one can obtain a reaso...

Fehrer, F; Reinhard, P G; Suraud, E

2005-01-01

145

Modeling Na clusters in Ar matrices  

NASA Astrophysics Data System (ADS)

We present a microscopic model for Na clusters embedded in raregas matrices. The valence electrons of the Na cluster are described by time-dependent density-functional theory at the level of the local-density approximation (LDA). Particular attention is paid to the semi-classical picture in terms of Vlasov-LDA. The Na+ ions and Ar atoms are handled as classical particles whereby the Ar atoms carry two degrees of freedom, position and dipole polarization. The interaction between Na+ ions and electrons is mediated through local pseudo-potentials. The coupling to the Ar atoms is described by (long-range) polarization potentials and (short-range) repulsive cores. The ingredients are taken from elsewhere developed standards. A final fine-tuning is performed using the NaAr molecule as benchmark. The model is then applied to embedded systems Na8ArN. By close comparison with quantum-mechanical results, we explore the capability of the Vlasov-LDA to describe such embedded clusters. We show that one can obtain a reasonable description by appropriate adjustments in the fine-tuning phase of the model.

Fehrer, F.; Mundt, M.; Reinhard, P.-G.; Suraud, E.

2005-07-01

146

Deliquescence of NaCl-NaNO3, KNO3-NaNO3, and NaCl-KNO3 Salt Mixtures From 90 to 120?C  

SciTech Connect

We conducted reversed deliquescence experiments in saturated NaCl-NaNO{sub 3}-H{sub 2}O, KNO{sub 3}-NaNO{sub 3}-H{sub 2}O, and NaCl-KNO{sub 3}-H{sub 2}O systems from 90 to 120 C as a function of relative humidity and solution composition. NaCl, NaNO{sub 3}, and KNO{sub 3} represent members of dust salt assemblages that are likely to deliquesce and form concentrated brines on high-level radioactive waste package surfaces in a repository environment at Yucca Mountain, NV, USA. Discrepancy between model prediction and experimental code can be as high as 8% for relative humidity and 50% for dissolved ion concentration. The discrepancy is attributed primarily to the use of 25 C models for Cl-NO{sub 3} and K-NO{sub 3} ion interactions in the current Yucca Mountain Project high-temperature Pitzer model to describe the non-ideal behavior of these highly concentrated solutions.

Carroll, S A; Craig, L; Wolery, T J

2004-10-20

147

Determination of Na 2O from sodium aluminate NaAlO 2  

Microsoft Academic Search

The aim of the work was to find a suitable method and conditions for determining Na2O wt.% from NaAlO2. Problems were encountered while titrating NaAlO2 with hydrochloric acid. The problematic area was the pH range 4–10 where aluminum precipitates as hydroxides. The different species of the aluminate solution were determined using potentiometric and complexometric titrations. The equivalent point of the

Teemu Näykki; Anttila Raimo; Perämäki Paavo; Kurikka Antero; Nikula Päivi

2000-01-01

148

Na+, K+, and Cl- transport in resting pancreatic acinar cells  

PubMed Central

To understand the role of Na+, K+, and Cl- transporters in fluid and electrolyte secretion by pancreatic acinar cells, we studied the relationship between them in resting and stimulated cells. Measurements of [Cl-]i in resting cells showed that in HCO3(-)-buffered medium [Cl- ]i and Cl- fluxes are dominated by the Cl-/HCO3- exchanger. In the absence of HCO3-, [Cl-]i is regulated by NaCl and NaK2Cl cotransport systems. Measurements of [Na+]i showed that the Na(+)-coupled Cl- transporters contributed to the regulation of [Na+]i, but the major Na+ influx pathway in resting pancreatic acinar cells is the Na+/H+ exchanger. 86Rb influx measurements revealed that > 95% of K+ influx is mediated by the Na+ pump and the NaK2Cl cotransporter. In resting cells, the two transporters appear to be coupled through [K+]i in that inhibition of either transporter had small effect on 86Rb uptake, but inhibition of both transporters largely prevented 86Rb uptake. Another form of coupling occurs between the Na+ influx transporters and the Na+ pump. Thus, inhibition of NaK2Cl cotransport increased Na+ influx by the Na+/H+ exchanger to fuel the Na+ pump. Similarly, inhibition of Na+/H+ exchange increased the activity of the NaK2Cl cotransporter. The combined measurements of [Na+]i and 86Rb influx indicate that the Na+/H+ exchanger contributes twice more than the NaK2Cl cotransporter and three times more than the NaCl cotransporter and a tetraethylammonium-sensitive channel to Na+ influx in resting cells. These findings were used to develop a model for the relationship between the transporters in resting pancreatic acinar cells. PMID:8786358

1995-01-01

149

FXYD Proteins Stabilize Na,K-ATPase  

PubMed Central

FXYD proteins are a family of seven small regulatory proteins, expressed in a tissue-specific manner, that associate with Na,K-ATPase as subsidiary subunits and modulate kinetic properties. This study describes an additional property of FXYD proteins as stabilizers of Na,K-ATPase. FXYD1 (phospholemman), FXYD2 (? subunit), and FXYD4 (CHIF) have been expressed in Escherichia coli and purified. These FXYD proteins associate spontaneously in vitro with detergent-soluble purified recombinant human Na,K-ATPase (?1?1) to form ?1?1FXYD complexes. Compared with the control (?1?1), all three FXYD proteins strongly protect Na,K-ATPase activity against inactivation by heating or excess detergent (C12E8), with effectiveness FXYD1 > FXYD2 ? FXYD4. Heating also inactivates E1 ? E2 conformational changes and cation occlusion, and FXYD1 protects strongly. Incubation of ?1?1 or ?1?1FXYD complexes with guanidinium chloride (up to 6 m) causes protein unfolding, detected by changes in protein fluorescence, but FXYD proteins do not protect. Thus, general protein denaturation is not the cause of thermally mediated or detergent-mediated inactivation. By contrast, the experiments show that displacement of specifically bound phosphatidylserine is the primary cause of thermally mediated or detergent-mediated inactivation, and FXYD proteins stabilize phosphatidylserine-Na,K-ATPase interactions. Phosphatidylserine probably binds near trans-membrane segments M9 of the ? subunit and the FXYD protein, which are in proximity. FXYD1, FXYD2, and FXYD4 co-expressed in HeLa cells with rat ?1 protect strongly against thermal inactivation. Stabilization of Na,K-ATPase by three FXYD proteins in a mammalian cell membrane, as well the purified recombinant Na,K-ATPase, suggests that stabilization is a general property of FXYD proteins, consistent with a significant biological function. PMID:21228272

Mishra, Neeraj Kumar; Peleg, Yoav; Cirri, Erica; Belogus, Talya; Lifshitz, Yael; Voelker, Dennis R.; Apell, Hans-Juergen; Garty, Haim; Karlish, Steven J. D.

2011-01-01

150

Reconstitution of intestinal Na(+)-phosphate cotransporter.  

PubMed

The rabbit intestinal brush-border membrane Na(+)-phosphate cotransporter was purified from sodium dodecyl sulfate (SDS)-brush-border membrane vesicles (BBMV) protein (SDS-treated Ca(2+)-precipitated BBMV) by a three-column chromatography protocol. The purification included a preparative scale chromatofocusing chromatography column over the pH range from 7.4 to 4 after solubilization in 3-[(3-cholamidopropyl)-diamethylammonia]-1-propanesulfonate (CHAPS), a chromatofocusing column over the pH range from 5.6 to 4 after solubilization in n-octyl glucoside, and gel filtration chromatography on a Sephacryl S-200 column. Verification of Na(+)-phosphate cotransporter purification involved substrate affinities, substrate stoichiometry, and inhibitor sensitivity after proteoliposome reconstitution and SDS-polyacrylamide gel electrophoresis (PAGE). After gel filtration Na(+)-dependent phosphate uptake was 3,300-fold enriched compared with the cell homogenate. A single 130-kDa polypeptide was visualized by SDS-PAGE under reducing conditions using silver stain. The coenrichment of this 130-kDa polypeptide and proteoliposome reconstituted Na(+)-dependent phosphate uptake suggest that the intestinal brush-border membrane Na(+)-phosphate cotransporter has been purified and proteoliposome reconstituted. PMID:8476048

Peerce, B E; Cedilote, M; Seifert, S; Levine, R; Kiesling, C; Clarke, R D

1993-04-01

151

Brief Communications Inwardly Permeating Na Ions Generate the Voltage  

E-print Network

Brief Communications Inwardly Permeating Na Ions Generate the Voltage Dependence of Resurgent Na measured voltage-clamped currents in different Na gradients to test the role of permeating ionsNaionsoccupyingtheexternalpore.Thelackofvoltagesensitivityoftheblockingproteinsuggeststhattheblocker'sbinding site lies outside the membrane field, in the permeation pathway. Introduction Resurgent Na current

Raman, Indira M.

152

Interpretation of the photoelectron spectra of superalkali species: Na3O and Na3O-  

NASA Astrophysics Data System (ADS)

Recently measured photoelectron spectra of the Na3O- anion have been interpreted with the aid of ab initio electron propagator calculations. As in the case of the Li3O-, we propose that the photoionization of ground and excited neutral states, in a sequential two photon absorption mechanism, plays a role in the interpretation of the observed spectrum. The lowest vertical electron detachment energy of Na3O- corresponds to a Dyson orbital that is composed chiefly of diffuse Na s functions and connects a D3h singlet anion to an uncharged species with the same point group. Electron binding energies of isomers of the anion with different point groups or multiplicities have been considered. The relative magnitudes of the ionization energies of the neutral Li3O and Na3O species are also discussed. Whereas the most recent experimental data hold that Na3O has the higher ionization energy, this work asserts the opposite trend.

Zein, S.; Ortiz, J. V.

2012-06-01

153

Na+/K+-ATPase: Activity and inhibition  

NASA Astrophysics Data System (ADS)

The aim of the study was to give an overview of the mechanism of inhibition of Na+/K+-ATPase activity induced by some specific and non specific inhibitors. For this purpose, the effects of some ouabain like compounds (digoxin, gitoxin), noble metals complexes ([PtCl2DMSO2], [AuCl4]-, [PdCl4]2-, [PdCl(dien)]+, [PdCl(Me4dien)]+), transition metal ions (Cu2+, Zn2+, Fe2+, Co2+), and heavy metal ions (Hg2+, Pb2+, Cd2+) on the activity of Na+/K+-ATPase from rat synaptic plasma membranes (SPM), porcine cerebral cortex and human erythrocytes were discussed.

?olovi?, M.; Krsti?, D.; Krinulovi?, K.; Momi?, T.; Savi?, J.; Vuja?i?, A.; Vasi?, V.

2009-09-01

154

Involvement of na in active uptake of pyruvate in mesophyll chloroplasts of some c(4) plants : na/pyruvate cotransport.  

PubMed

An artificial Na(+) gradient across the envelope (Na(+) jump) enhanced pyruvate uptake in the dark into mesophyll chloroplasts of a C(4) plant, Panicum miliaceum (NAD-malic enzyme type) (J Ohnishi, R Kanai [1987] FEBS Lett 219:347). In the present study, (22)Na(+) and pyruvate uptake were examined in mesophyll chloroplasts of several species of C(4) plants. Enhancement of pyruvate uptake by a Na(+) jump in the dark was also seen in mesophyll chloroplasts of Urochloa panicoides and Panicum maximum (phosphoenolpyruvate carboxykinase types) but not in Zea mays or Sorghum bicolor (NADP-malic enzyme types). In mesophyll chloroplasts of P. miliaceum and P. maximum, pyruvate in turn enhanced Na(+) uptake in the dark when added together with Na(+). When flux of endogenous Na(+) was measured in these mesophyll chloroplasts preincubated with (22)Na(+), pyruvate addition induced Na(+) influx, and the extent of the pyruvate-induced Na(+) influx positively correlated with that of pyruvate uptake. A Na(+)/H(+) exchange ionophore, monensin, nullified all the above mutual effects of Na(+) and pyruvate in mesophyll chloroplasts of P. miliaceum, while it accelerated Na(+) uptake and increased equilibrium level of chloroplast (22)Na(+). Measurements of initial uptake rates of pyruvate and Na(+) gave a stoichiometry close to 1:1. These results point to Na(+)/pyruvate cotransport into mesophyll chloroplasts of some C(4) plants. PMID:16667876

Ohnishi, J; Flügge, U I; Heldt, H W; Kanai, R

1990-11-01

155

POŠKODBE S TRAKTORJI IN OBDELOVALNIMI STROJI PRI OTROCIH TRACTOR AND FARM MACHINERY RELATED INJURIES IN CHILDREN  

Microsoft Academic Search

Zajeli smo 45 otrok, starih povpre?no 7,2 leta (SD 2,3; razpon 17 mesecev do 14 let). De?- kov je bilo 39 (87%), deklic 6 (13%). S traktorji je bilo poškodovanih 28 (62%) otrok, kot potniki na poljedelskem vozu 4 (10%), z obdelovalnimi stroji 8 (18%), z delovnim strojem ali s poljedelskim orodjem 5 (10%). Lahkih poškodb je bilo 19 (42%),

Irena Gracar

156

Characterisation of sodium cations in dehydrated zeolite NaX by 23Na NMR spectroscopy.  

PubMed

23Na MAS, 2D nutation MAS, and DOR NMR spectroscopy has been applied to characterise the location of sodium cations in dehydrated zeolite NaX (Si/Al = 1.23). The 23Na MAS NMR spectra recorded at three different magnetic field strengths were decomposed by computer simulation into five lines, which were attributed to five crystallographically distinct cation sites known from X-ray diffraction studies. The assignments of the lines follow from electric field gradient calculations at the 23Na nuclei applying a simple point charge model based on crystal structure data. A weak Gaussian line at low field (delta iso = -6 ppm) is assigned to sodium cations at site I, two broad quadrupole patterns at the high-field side of the spectra are attributed to site I' (delta iso = -19 ppm, QCC = 5.2 MHz, eta = 0) and site II cations (delta iso = -15 ppm, QCC = 4.6 MHz, eta = 0), and two quadrupolar lines dominating the central region of the spectra originate from Na+ at two different III' sites (delta iso = -13 and -29 ppm, QCC = 2.6 and 1.6 MHz, eta = 0.7 and 0.9, respectively). Na+ ions located on a second I' site could be identified from the DOR NMR spectra. The line assignment is further corroborated by the reasonable agreement of the site occupancies estimated from the line intensities with those determined by X-ray diffraction. In addition, sodium site populations of five dehydrated zeolites NaX and NaY with Si/Al ratios between 1.09 and 4.0 were derived from the 23Na MAS NMR spectra. PMID:8986022

Feuerstein, M; Hunger, M; Engelhardt, G; Amoureux, J P

1996-11-01

157

Optics and Interferometry with Na2 Molecules  

Microsoft Academic Search

We have produced an intense, pure beam of sodium molecules (Na2) by using light forces to separate the atomic and molecular species in a seeded supersonic beam. We used diffraction from a microfabricated grating to study the atomic and molecular sodium in the beam. Using three of these gratings, we constructed a molecule interferometer with fully separated beams and high

Michael S. Chapman; Christopher R. Ekstrom; Troy D. Hammond; Richard A. Rubenstein; Jörg Schmiedmayer; Stefan Wehinger; David E. Pritchard

1995-01-01

158

Binding to Na+ exchanger regulatory factor 2  

E-print Network

Binding to Na+ /H+ exchanger regulatory factor 2 (NHERF2) affects trafficking and function in order to colonize and multiply in the human gut. Map, EspI and NleH1 are conserved EPEC effectors the trafficking of EspI to the Golgi and diminished the anti-apoptotic activity of NleH1. The binding of multiple

Hall, Randy A

159

master's degree NaNotechNology  

E-print Network

master's degree NaNotechNology When choosing a Master's programme, it is wise to look one step topical, incorporating the latest developments in applied physics, nanotechnology, chemical engineering projects will be carried out at the MESA+ institute for nanotechnology, or the MIRA institute

Twente, Universiteit

160

IV laser spectroscopy on NaI  

NASA Astrophysics Data System (ADS)

Using frequency-double laser radiation, fragmentary rotational fine structure was observed and assigned in the electronic transition 0 + - X 1? + of NaI for the first time. Adopting the semiclassical model of Child for predissociating states, molecular parameters were determined for the limiting diabatic and adiabatc states above the dissociation energy.

Schaefer, S. H.; Bender, D.; Tiemann, E.

1982-10-01

161

Single ionization of Na(3s) and Na*(3p) by low energy ion impact  

NASA Astrophysics Data System (ADS)

Single ionization cross sections for He2+ and O6+ impact on Na*(3p) in the 2-10 keV amu-1 energy range have been measured using recoil-ion momentum spectroscopy of cold Na atoms. The present results are compared with our previous data on Na(3s). It is found that ionization of these weakly bound alkali systems yields larger cross sections than predicted from scaling laws derived from H and He targets. Also information on the ejected electron momenta could be obtained from the recoil spectra.

Knoop, S.; Hasan, V. G.; Ott, H.; Morgenstern, R.; Hoekstra, R.

2006-04-01

162

Utjecaj ošte?enja lista še?erne repe u vegetaciji na prinos i kvalitetu korijena  

Microsoft Academic Search

Sažetak Šteta u vidu pada prinosa i kvalitete korijena koja nastaje zbog ošte?enja lista še?erne repe neadekvatnom primjenom fungicida u borbi protiv pjegavosti lista istraživali smo u razdoblju od 2005-2007 god. U istraživanja, zbog nejednake otpornosti hibrida še?erne repe protiv uzro?nika pjegavosti patogene gljive Cercospora beticola Sacc. bilo je uklju?eno 23 hibrida, koji se ina?e koriste u širokoj proizvodnji. Istraživanja

Andrija KRISTEK; Suzana KRISTEK; Josip Juraj

163

Luminescence of NaCl:Tl and NaCl:In at high pressure  

NASA Astrophysics Data System (ADS)

The effects of pressure up to 40 kbar and temperatures down to 100 °K on the intensity and lifetime of NaCa:Tl+ and NaCl:In has been studied. A kinetic model describing these luminescent properties has been proposed. The 3Tlu level is in thermal equilibrium with 3Alu. Both 3Tlu and 3Alu have nonradiative pathways to the ground state 1Alg. In addition 3Tlu is radiatively coupled to the ground state. Increasing pressure increases the radiative rate from 3Tlu to 1Alg and has no effect on the equilibrium between 3Tlu and 3Alu. The back feeding rate from 3Alu to 3Tlu is temperature sensitive for NaCl:Tl but not for NaCl:In.

Littman, J. E.; Drickamer, H. G.

1982-03-01

164

Kinetics of Na(+)-dependent conformational changes of rabbit kidney Na+,K(+)-ATPase.  

PubMed Central

The kinetics of Na(+)-dependent partial reactions of the Na+,K(+)-ATPase from rabbit kidney were investigated via the stopped-flow technique, using the fluorescent labels N-(4-sulfobutyl)-4-(4-(p-(dipentylamino)phenyl)butadienyl)py ridinium inner salt (RH421) and 5-iodoacetamidofluorescein (5-IAF). When covalently labeled 5-IAF enzyme is mixed with ATP, the two labels give almost identical kinetic responses. Under the chosen experimental conditions two exponential time functions are necessary to fit the data. The dominant fast phase, 1/tau 1 approximately 155 s-1 for 5-IAF-labeled enzyme and 1/tau 1 approximately 200 s-1 for native enzyme (saturating [ATP] and [Na+], pH 7.4 and 24 degrees C), is attributed to phosphorylation of the enzyme and a subsequent conformational change (E1ATP(Na+)3-->E2P(Na+)3 + ADP). The smaller amplitude slow phase, 1/tau 2 = 30-45 s-1, is attributed to the relaxation of the dephosphorylation/rephosphorylation equilibrium in the absence of K+ ions (E2P<==>E2). The Na+ concentration dependence of 1/tau 1 showed half-saturation at a Na+ concentration of 6-8 mM, with positive cooperatively involved in the occupation of the Na+ binding sites. The apparent dissociation constant of the high-affinity ATP-binding site determined from the ATP concentration dependence of 1/tau 1 was 8.0 (+/- 0.7) microM. It was found that P3-1-(2-nitrophenyl)ethyl ATP, tripropylammonium salt (NPE-caged ATP), at concentrations in the hundreds of micromolar range, significantly decreases the value of 1/tau 1, observed. This, as well as the biexponential nature of the kinetic traces, can account for previously reported discrepancies in the rates of the reactions investigated. PMID:9726935

Clarke, R J; Kane, D J; Apell, H J; Roudna, M; Bamberg, E

1998-01-01

165

Na/beta-alumina/NaAlCl4, Cl2/C circulating cell  

NASA Technical Reports Server (NTRS)

A study was made of a high specific energy battery based on a sodium negative electrode and a chlorine positive electrode with molten AlCl3-NaCl electrolyte and a solid beta alumina separator. The basic performance of a Na beta-alumina NaAlCl4, Cl2/C circulating cell at 200 C was demonstrated. This cell can be started at 150 C. The use of melting sodium chloroaluminate electrolyte overcomes some of the material problems associated with the high working temperatures of present molten salt systems, such as Na/S and LiAl/FeS, and retains the advantages of high energy density and relatively efficient electrode processes. Preliminary investigations were conducted on a sodium-chlorine static cell, material compability, electrode design, wetting, and theoretical calculations to assure a better chance of success before assembling a Na/Cl2 circulating cell. Mathematical models provide a theoretical explanation for the performance of the NaCl2 battery. The results of mathematical models match the experimental results very well. According to the result of the mathematical modeling, an output at 180 mA/sq cm and 3.2 V can be obtained with optimized cell design.

Cherng, Jing-Yih; Bennion, Douglas N.

1987-01-01

166

Je-S Guide to User Account Registration G91v4/ML/Je-S UserAccGuide February 2011 Page 1 of 9  

E-print Network

made by one of the UK Research Councils. All funding applications made to the following Research Councils are submitted electronically, using the Full Economic Costing (FEC) methodology Arts Funding Guide BBSRC Research Grants Guide EPSRC Funding Guide ESRC Research Funding Guide MRC Research

167

Amino group modification of (Na + +K + )ATPase  

Microsoft Academic Search

The effects of three amino group reagents on the activity of (Na++K+)-ATPase3 and its component K+-stimulatedp-nitrophenylphosphatase activity from rabbit kidney outer medulla have been studied. All three reagents cause inactivation of the enzyme. Modification of amino groups with trinitrobenzene sulfonic acid yields kinetics of inactivation of both activities, which depend on the type and concentration of the ligands present. In

J. J. H. H. M. De Pont; S. E. Emst-De Vries; S. L. Bonting

1984-01-01

168

LA GACETA 107 RESE ~NA DE LIBROS  

E-print Network

LA GACETA 107 RESE ~NA DE LIBROS DYNAMICAL SYSTEMS WITH APPLICATIONS USING MAPPLE Autor: Stephen libro publicado por alg´un autor ruso, no siempre f´acil de desci- frar. En los ´ultimos a~nos, sin los muchos libros que existen en el mercado y que van apareciendo cada d´ia. Una buena elecci´on ser

Rey Juan Carlos, Universidad

169

Inelastic and reactive collisions with polarized excited Na atoms  

SciTech Connect

Polarization effects in inelastic collisions of laser state-prepared Na(3/sup 2/P, M/sub J/) with Na/sup +/ leading to Na(3/sup 2/D) or Na(3/sup 2/S) are discussed for the energy range E/sub cm/ = 5-47.5eV. Studies with linearly polarized light can be explained with a simple ''locking'' model of the Na(P)-orbital. The investigations employing circularly polarized light are a very sensitive test of the models describing the nonadiabatic angular momentum coupling between electronic and nuclear motion. The dynamical effects of the electronic spin on the angular momentum transfer are discussed. Recent crossed-beam experiments on the Na + O/sub 2/ -> NaO = O reaction in the energy range E/sub cm/ = 0/3-0.8eV show a pronounced dependence on the electric electronic symmetry of Na. 17 refs., 11 figs.

Schmidt, H.; Hertel, I.V.; Lee, Y.T.

1985-07-01

170

An enhancement to the NA4 gear vibration diagnostic parameter  

NASA Technical Reports Server (NTRS)

A new vibration diagnostic parameter for health monitoring of gears, NA4*, is proposed and tested. A recently developed gear vibration diagnostic parameter NA4 outperformed other fault detection methods at indicating the start and initial progression of damage. However, in some cases, as the damage progressed, the sensitivity of the NA4 and FM4 parameters tended to decrease and no longer indicated damage. A new parameter, NA4* was developed by enhancing NA4 to improve the trending of the parameter. This allows for the indication of damage both at initiation and also as the damage progresses. The NA4* parameter was verified and compared to the NA4 and FM4 parameters using experimental data from single mesh spur and spiral bevel gear fatigue rigs. The primary failure mode for the test cases was naturally occurring tooth surface pitting. The NA4* parameter is shown to be a more robust indicator of damage.

Decker, Harry J.; Handschuh, Robert F.; Zakrajsek, James J.

1994-01-01

171

An enhancement to the NA4 gear vibration diagnostic parameter  

NASA Astrophysics Data System (ADS)

A new vibration diagnostic parameter for health monitoring of gears, NA4*, is proposed and tested. A recently developed gear vibration diagnostic parameter NA4 outperformed other fault detection methods at indicating the start and initial progression of damage. However, in some cases, as the damage progressed, the sensitivity of the NA4 and FM4 parameters tended to decrease and no longer indicated damage. A new parameter, NA4* was developed by enhancing NA4 to improve the trending of the parameter. This allows for the indication of damage both at initiation and also as the damage progresses. The NA4* parameter was verified and compared to the NA4 and FM4 parameters using experimental data from single mesh spur and spiral bevel gear fatigue rigs. The primary failure mode for the test cases was naturally occurring tooth surface pitting. The NA4* parameter is shown to be a more robust indicator of damage.

Decker, Harry J.; Handschuh, Robert F.; Zakrajsek, James J.

1994-07-01

172

Progress of the NA62 RICH detector  

NASA Astrophysics Data System (ADS)

NA62 is the last generation Kaon experiment at CERN. Its main goal is to collect about 100 K+??+?bar nu events with 10% background. This implies to collect more than 1013 K+ decays with a backgound rejection factor better than 1012. The challenging aspect of the experiment is the suppression of Kaon decays with branching ratioes up to 10 orders of magnitude higher than the signal and similar experimental signature, such as K+??+?. To this purpose good PID (Particle IDentification) and kinematic rejection are required. Precise timing is also needed to correctly associate the ?+ with the parent K+ in an high rate environment. A RICH (Ring Imaging Cherenkov) detector is proposed as PID element, to identify ? contaminating the ? sample in the 15-35 GeV/c momentum range with inefficiency lower than 1% and to measure the arrival time with precision smaller than 100 ps. It will also be a key element for the NA62 trigger. A vacuum-proof cylindrical vessel with 4 m diameter, 17 m long, will be filled with Neon gas at atmospheric pressure. The Cherenkov light will be reflected by a mosaic of 20 hexagonal mirrors with 17 m focal length and collected by 2000 photomultipliers. The progress in the construction of the detector is described: the installation will start in January 2014, with completion foreseen in time for the first physics run of the NA62 experiment in the fall of 2014.

Lenti, Massimo

2014-01-01

173

Resonances production from the NA60 experiment  

NASA Astrophysics Data System (ADS)

The NA60 experiment at the CERN SPS has studied light vector meson production in In-In collisions at 158A GeV. The ? meson was detected via both the K+K- and the ?+?- decay channels. The yields and inverse slope parameters of the mT spectra observed in the two channels are compatible within errors, different from the large discrepancies seen in Pb-Pb collisions between the hadronic (NA49) and dimuon (NA50) decay channels. In the invariant mass region 0.2 < M?? < 2.6 GeV, a strong excess of pairs above the sources that describe the mass spectrum in p-A collisions is observed. The mass spectrum for M?? < 1 GeV is consistent with a dominant contribution from the pion annihilation process ? + ? ? ? ? ?+?-. For M?? > 1 GeV, the excess is found to be prompt, with remarkable differences with respect to the Drell-Yan process. The Teff slope parameter, extracted from the transverse mass spectra, shows a rise with mass up to the ?, followed by a sudden decline for higher masses. The former is consistent with radial flow of a hadronic source, while the latter suggests a dominantly partonic emission source.

De Falco, A.

2012-11-01

174

Determining Neutron Multiplicity in MoNA  

NASA Astrophysics Data System (ADS)

The Modular Neutron Array, located at the NSCL at Michigan State University, is used in conjunction with the MSU/FSU Sweeper Magnet to study the breakup of neutron-rich nuclei. Fragmentation reactions create particle-unstable nuclei near the neutron dripline which spontaneously breakup by the decay of one or two neutrons with energies that reflect the nuclear structure of unbound excited and ground states. The neutrons continue forward into MoNA where their position and time are recorded, and the charged fragments' position and energy are measured by the array of detectors following the Sweeper Magnet. The neutron decay energies can then be determined using kinematic reconstruction of the breakup. It is important to distinguish between one- and two-neutron decays in MoNA. We have therefore developed an algorithm that helps determine neutron multiplicity, based on energy and momentum conservation restrictions for single neutrons scattering multiple times in MoNA. Results of this approach to the analysis of ^12Be and ^11Be decay will be presented.

Gillette, J.; Gardner, M.; Reed, A.; Rogers, W. F.; Mosby, S.

2007-10-01

175

Življenje in delo Jožefa Borovnjaka  

E-print Network

nemš?ino. V petem tematskem sklopu Franc Kuzmi? na enem mestu podaja sistema- ti?no urejeno bibliografijo Jožefa Bo- rovnjaka. Najprej so navedena njegova dela, temu pa je dodano še poglavje O Borovnjaku, kjer je prikazan abecedni seznam literature, ki... tako natan?no, sistemati?no in pri- merjalno predstavljeno, analizirano in zbrano na enem mestu. Jezik je, kakor na platnicah piše tudi Marko Jesenšek, res še vedno najpo- membnejši nacionalni atribut, ki nam še danes zagotavlja obstoj našega naroda...

Benko, Anja

2009-01-01

176

A 23Na magic angle spinning nuclear magnetic resonance, XANES, and high-temperature X-ray diffraction study of NaUO3, Na4UO5, and Na2U2O7.  

PubMed

The valence state of uranium has been confirmed for the three sodium uranates NaU(V)O3/[Rn](5f(1)), Na4U(VI)O5/[Rn](5f(0)), and Na2U(VI)2O7/[Rn](5f(0)), using X-ray absorption near-edge structure (XANES) spectroscopy. Solid-state (23)Na magic angle spinning nuclear magnetic resonance (MAS NMR) measurements have been performed for the first time, yielding chemical shifts at -29.1 (NaUO3), 15.1 (Na4UO5), and -14.1 and -19 ppm (Na1 8-fold coordinated and Na2 7-fold coordinated in Na2U2O7), respectively. The [Rn]5f(1) electronic structure of uranium in NaUO3 causes a paramagnetic shift in comparison to Na4UO5 and Na2U2O7, where the electronic structure is [Rn]5f(0). A (23)Na multi quantum magic angle spinning (MQMAS) study on Na2U2O7 has confirmed a monoclinic rather than rhombohedral structure with evidence for two distinct Na sites. DFT calculations of the NMR parameters on the nonmagnetic compounds Na4UO5 and Na2U2O7 have permitted the differentiation between the two Na sites of the Na2U2O7 structure. The linear thermal expansion coefficients of all three compounds have been determined using high-temperature X-ray diffraction: ?a = 22.7 × 10(-6) K(-1), ?b = 12.9 × 10(-6) K(-1), ?c = 16.2 × 10(-6) K(-1), and ?vol = 52.8 × 10(-6) K(-1) for NaUO3 in the range 298-1273 K; ?a = 37.1 × 10(-6) K(-1), ?c = 6.2 × 10(-6) K(-1), and ?vol = 81.8 × 10(-6) K(-1) for Na4UO5 in the range 298-1073 K; ?a = 6.7 × 10(-6) K(-1), ?b = 14.4 × 10(-6) K(-1), ?c = 26.8 × 10(-6) K(-1), ?? = -7.8 × 10(-6) K(-1), and ?vol = -217.6 × 10(-6) K(-1) for Na2U2O7 in the range 298-573 K. The ? to ? phase transition reported for the last compound above about 600 K was not observed in the present studies, either by high-temperature X-ray diffraction or by differential scanning calorimetry. PMID:24350659

Smith, A L; Raison, P E; Martel, L; Charpentier, T; Farnan, I; Prieur, D; Hennig, C; Scheinost, A C; Konings, R J M; Cheetham, A K

2014-01-01

177

Na+/H+ and Na+/NH+4 activities of zebrafish NHE3b expressed in Xenopus oocytes.  

PubMed

Zebrafish Na(+)/H(+) exchanger 3b (zNHE3b) is highly expressed in the apical membrane of ionocytes where Na(+) is absorbed from ion-poor fresh water against a concentration gradient. Much in vivo data indicated that zNHE3b is involved in Na(+) absorption but not leakage. However, zNHE3b-mediated Na(+) absorption has not been thermodynamically explained, and zNHE3b activity has not been measured. To address this issue, we overexpressed zNHE3b in Xenopus oocytes and characterized its activity by electrophysiology. Exposure of zNHE3b oocytes to Na(+)-free media resulted in significant decrease in intracellular pH (pH(i)) and intracellular Na(+) activity (aNa(i)). aNa(i) increased significantly when the cytoplasm was acidified by media containing CO?-HCO?(-) or butyrate. Activity of zNHE3b was inhibited by amiloride or 5-ethylisopropyl amiloride (EIPA). Although the activity was accompanied by a large hyperpolarization of ?50 mV, voltage-clamp experiments showed that Na(+)/H(+) exchange activity of zNHE3b is electroneutral. Exposure of zNHE3b oocytes to medium containing NH?/NH?(+) resulted in significant decreases in pH(i) and aNa(i) and significant increase in intracellular NH?(+) activity, indicating that zNHE3b mediates the Na(+)/NH?(+) exchange. In low-Na(+) (0.5 mM) media, zNHE3b oocytes maintained aNa(i) of 1.3 mM, and Na(+)-influx was observed when pHi was decreased by media containing CO?-HCO?(-) or butyrate. These results provide thermodynamic evidence that zNHE3b mediates Na(+) absorption from ion-poor fresh water by its Na(+)/H(+) and Na(+)/NH?(+) exchange activities. PMID:24401990

Ito, Yusuke; Kato, Akira; Hirata, Taku; Hirose, Shigehisa; Romero, Michael F

2014-03-01

178

Angiotensin II-induced hypertension increases plasma membrane Na pump activity by enhancing Na entry in rat thick ascending limbs.  

PubMed

Thick ascending limbs (TAL) reabsorb 30% of the filtered NaCl load. Na enters the cells via apical Na-K-2Cl cotransporters and Na/H exchangers and exits via basolateral Na pumps. Chronic angiotensin II (ANG II) infusion increases net TAL Na transport and Na apical entry; however, little is known about its effects on the basolateral Na pump. We hypothesized that in rat TALs Na pump activity is enhanced by ANG II-infusion, a model of ANG II-induced hypertension. Rats were infused with 200 ng·kg(-1)·min(-1) ANG II or vehicle for 7 days, and TAL suspensions were obtained. We studied plasma membrane Na pump activity by measuring changes in 1) intracellular Na (Nai) induced by ouabain; and 2) ouabain-sensitive oxygen consumption (QO2). We found that the ouabain-sensitive rise in Nai in TALs from ANG II-infused rats was 12.8 ± 0.4 arbitrary fluorescent units (AFU)·mg(-1)·min(-1) compared with only 9.9 ± 1.1 AFU·mg(-1)·min(-1) in controls (P < 0.024). Ouabain-sensitive oxygen consumption was 17 ± 5% (P < 0.043) greater in tubules from ANG II-treated than vehicle rats. ANG II infusion did not alter total Na pump expression, the number of Na pumps in the plasma membrane, or the affinity for Na. When furosemide (1.1 mg·kg(-1)·day(-1)) was coinfused with ANG II, no increase in plasma membrane Na pump activity was observed. We concluded that in ANG II-induced hypertension Na pump activity is increased in the plasma membrane of TALs and that this increase is caused by the chronically enhanced Na entry occurring in this model. PMID:23986517

Gonzalez-Vicente, Agustin; Garvin, Jeffrey L

2013-11-01

179

23Na multiple quantum filtered NMR characterisation of Na+ binding and dynamics in animal cells: a comparative study and effect of Na+/Li + competition.  

PubMed

Double quantum and triple quantum filtered (23)Na nuclear magnetic resonance techniques were used to characterise in detail the isotropic and anisotropic binding and dynamics of intra- and extracellular Na(+) in different cellular systems, in the absence and presence of Li(+). The kinetics of Li(+) influx by different cell types was evaluated. At steady state, astrocytes accumulated more Li(+) than red blood cells (RBCs), while a higher intracellular Li(+) concentration was found in chromaffin than in SH-SY5Y cells. Anisotropic and isotropic motions were detected for extracellular Na(+) in all cellular systems studied. Isotropic intracellular Na(+) motions were observed in all types of cells, while anisotropic Na(+) motions in the intracellular compartment were only detected in RBCs. (23)Na triple quantum signal efficiency for intracellular Na(+) was SH-SY5Y > chromaffin > RBCs, while the reverse order was observed for the extracellular ions. (23)Na double quantum signal efficiency for intracellular Na(+) was non-zero only in RBCs, and for extracellular Na(+) the order RBCs > chromaffin > SH-SY5Y cells was observed. Li(+) loading generally decreased intracellular Na(+) isotropic movements in the cells, except for astrocytes incubated with a low Li(+) concentration and increased anisotropic intracellular Na(+) movements in RBCs. Li(+) effects on the extracellular signals were more complex, reflecting Li(+)/Na(+) competition for isotropic and anisotropic binding sites at the extracellular surface of cell membranes and also at the surface of the gel used for cell immobilisation. These results are relevant and contribute to the interpretation of the in vivo pharmacokinetics and sites of Li(+) action. PMID:23563802

Fonseca, Carla P; Fonseca, Luís L; Montezinho, Liliana P; Alves, Paula M; Santos, Helena; Castro, M Margarida C A; Geraldes, Carlos F G C

2013-07-01

180

Fonološki opis govora Dovjega  

E-print Network

? m l r n j (?)8 3.2.2 Nezvo?niki p b f t d c s z ? (?)9 š ž k g x 3.3 Naglas 3.3.1 Naglas ni vezan na dolo?eno mesto v besedah – naglasno mesto je svo- bodno. 3.3.2 Govor pozna kvantitetno opozicijo (dolge naglašene, kratke naglašene in kratke... samoglasniki – za govor je zna- ?ilno švapanje (?í?d?a ‘videla’, ?à?š ‘laž’, m?a?t?? ‘mlati?’).12 10 Gradivo je zapisano tako kot v rokopisnem gradivu iz arhiva za SLA, le zapis tonemov je posodobljen (? > ??, ? > ??, ? > ?V). 11 Informator iz Plavškega...

Škofic, Jožica

2013-09-17

181

Advanced Intermediate-Temperature Na-S Battery  

SciTech Connect

In this study, we reported an intermediate-temperature (~150°C) sodium-sulfur (Na-S) battery. With a reduced operating temperature, this novel battery can potentially reduce the cost and safety issues associated with the conventional high-temperature (300~350°C) Na-S battery. A dense ?"-Al2O3 solid membrane and tetraglyme were utilized as the electrolyte separator and catholyte solvent in this battery. Solubility tests indicated that cathode mixture of Na2S4 and S exhibited extremely high solubility in tetraglyme (e.g., > 4.1 M for Na2S4 + 4 S). CV scans of Na2S4 in tetraglyme revealed two pairs of redox couples with peaks at around 2.22 and 1.75 V, corresponding to the redox reactions of polysulfide species. The discharge/charge profiles of the Na-S battery showed a slope region and a plateau, indicating multiple steps and cell reactions. In-situ Raman measurements during battery operation suggested that polysulfide species were formed in the sequence of Na2S5 + S ? Na2S5 + Na2S4? Na2S4 + Na2S2 during discharge and in a reverse order during charge. This battery showed dramatic improvement in rate capacity and cycling stability over room-temperature Na-S batteries, which makes it attractive for renewable energy integration and other grid related applications.

Lu, Xiaochuan; Kirby, Brent W.; Xu, Wu; Li, Guosheng; Kim, Jin Yong; Lemmon, John P.; Sprenkle, Vincent L.; Yang, Zhenguo (Gary) [Gary

2013-01-01

182

Influence of Salt Purity on Na+ and Palmitic Acid Interactions  

E-print Network

Influence of Salt Purity on Na+ and Palmitic Acid Interactions Zishuai Huang, Wei Hua, Dominique of salt purity on the interactions between Na+ ions and the carboxylate (COO- ) head group of palmitic frequency generation (VSFG) spectroscopy. Ultrapure (UP) and ACS grade NaCl salts are used for aqueous

183

Rigidification of the autolysis loop enhances Na+ binding to thrombin  

E-print Network

Rigidification of the autolysis loop enhances Na+ binding to thrombin Nicola Pozzi, Raymond Chen April 2011 Keywords: Thrombin Na+ binding Allostery X-ray crystallography Binding of Na+ to thrombin+ with thrombin and possibly increase catalytic activity under physiological conditions have so far been

Di Cera, Enrico

184

Electrochemical Properties of Monoclinic NaNiO2  

E-print Network

Monoclinic NaNiO[subscript 2] is re-investigated as a positive electrode material for sodium ion batteries. We observe reversible Na extraction and insertion of about 120 mAh/g when NaNiO[subscript 2] is cycled between ...

Vassilaras, Plousia E.

185

Zero-gravity growth of NaF-NaCl eutectics in the NASA Skylab program  

NASA Technical Reports Server (NTRS)

Continuous and discontinuous NaF fibers, embedded in a NaCl matrix, were produced in space and on earth. The production of continuous fibers in a eutectic mixture is attributed to the absence of convection current in the liquid during solidification in space. Image transmission and optical transmittance measurements of transverse sections of the space-grown and earth-grown ingots were made with a light microscope and a spectrometer. It is shown that better optical properties were obtained from samples grown in space. This was attributed to a better alignment of NaF fibers along the ingot axis. A new concept is advanced to explain the phenomenon of transmittance versus far infrared wavelength of the directionally solidified NaCl-NaF eutectic in terms of the two-dimensional Bragg Scattering and the polarization effect of Rayleigh scattering. This concept can be applied to other eutectic systems as long as the index of refraction of the matrix over a range of wavelengths is known. Experimental data are in agreement with the theoretical prediction.

Yue, A. S.; Allen, F. G.; Yu, J. G.

1976-01-01

186

Route, mechanism, and implications of proton import during Na+/K+ exchange by native Na+/K+-ATPase pumps  

PubMed Central

A single Na+/K+-ATPase pumps three Na+ outwards and two K+ inwards by alternately exposing ion-binding sites to opposite sides of the membrane in a conformational sequence coupled to pump autophosphorylation from ATP and auto-dephosphorylation. The larger flow of Na+ than K+ generates outward current across the cell membrane. Less well understood is the ability of Na+/K+ pumps to generate an inward current of protons. Originally noted in pumps deprived of external K+ and Na+ ions, as inward current at negative membrane potentials that becomes amplified when external pH is lowered, this proton current is generally viewed as an artifact of those unnatural conditions. We demonstrate here that this inward current also flows at physiological K+ and Na+ concentrations. We show that protons exploit ready reversibility of conformational changes associated with extracellular Na+ release from phosphorylated Na+/K+ pumps. Reversal of a subset of these transitions allows an extracellular proton to bind an acidic side chain and to be subsequently released to the cytoplasm. This back-step of phosphorylated Na+/K+ pumps that enables proton import is not required for completion of the 3 Na+/2 K+ transport cycle. However, the back-step occurs readily during Na+/K+ transport when external K+ ion binding and occlusion are delayed, and it occurs more frequently when lowered extracellular pH raises the probability of protonation of the externally accessible carboxylate side chain. The proton route passes through the Na+-selective binding site III and is distinct from the principal pathway traversed by the majority of transported Na+ and K+ ions that passes through binding site II. The inferred occurrence of Na+/K+ exchange and H+ import during the same conformational cycle of a single molecule identifies the Na+/K+ pump as a hybrid transporter. Whether Na+/K+ pump–mediated proton inflow may have any physiological or pathophysiological significance remains to be clarified. PMID:24688018

Vedovato, Natascia

2014-01-01

187

Na double-edge magneto-optic filter for Na lidar profiling of wind and temperature in the  

E-print Network

Na double-edge magneto-optic filter for Na lidar profiling of wind and temperature in the lower Doppler lidars measure wind and temperature simultaneously in the meso- sphere and lower thermosphere [1-edge magneto-optic filter is proposed for incorporation into the receiver of a three-frequency Na Doppler lidar

Chu, Xinzhao

188

Determination of Na(2)O from sodium aluminate NaAlO(2).  

PubMed

The aim of the work was to find a suitable method and conditions for determining Na(2)O wt.% from NaAlO(2). Problems were encountered while titrating NaAlO(2) with hydrochloric acid. The problematic area was the pH range 4-10 where aluminum precipitates as hydroxides. The different species of the aluminate solution were determined using potentiometric and complexometric titrations. The equivalent point of the potentiometric titration was detected using Gran's plotting method. Precipitation of aluminum hydroxides did not interfere with titrations, because in potentiometric titrations the pH value was over 10 and in complexometric titrations the pH was 4. The results were accurate and determinations were easy to carry out. Sodium was also determined by DCP-AES. PMID:18968035

Näykki, T; Raimo, A; Paavo, P; Antero, K; Päivi, N

2000-07-31

189

Shank2 redistributes with NaPilla during regulated endocytosis  

PubMed Central

Serum phosphate levels are acutely impacted by the abundance of sodium-phosphate cotransporter IIa (NaPiIIa) in the apical membrane of renal proximal tubule cells. PSD-95/Disks Large/Zonula Occludens (PDZ) domain-containing proteins bind NaPiIIa and likely contribute to the delivery, retention, recovery, and trafficking of NaPiIIa. Shank2 is a distinctive PDZ domain protein that binds NaPiIIa. Its role in regulating NaPiIIa activity, distribution, and abundance is unknown. In the present in vivo study, rats were maintained on a low-phosphate diet, and then plasma phosphate levels were acutely elevated by high-phosphate feeding to induce the recovery, endocytosis, and degradation of NaPiIIa. Western blot analysis of renal cortical tissue from rats given high-phosphate feed showed NaPiIIa and Shank2 underwent degradation. Quantitative immunofluorescence analyses, including microvillar versus intracellular intensity ratios and intensity correlation quotients, showed that Shank2 redistributed with NaPiIIa during the time course of NaPiIIa endocytosis. Furthermore, NaPiIIa and Shank2 trafficked through distinct endosomal compartments (clathrin, early endosomes, lysosomes) with the same temporal pattern. These in vivo findings indicate that Shank2 is positioned to coordinate the regulated endocytic retrieval and downregulation of NaPiIIa in rat renal proximal tubule cells. PMID:20810910

Dobrinskikh, Evgenia; Giral, Hector; Caldas, Yupanqui A.; Levi, Moshe

2010-01-01

190

Standardization method of ²²Na using two NaI(Tl) scintillation detectors.  

PubMed

A new standardization method for a sealed (22)Na point source was developed utilizing two NaI(Tl) scintillation detectors. In the proposed method, the count rates of annihilation radiation, gamma rays, their coincidence sum, and the coincidences between the two detectors are used. The equations from which the source activity can be deduced are derived in this work. A series of EGS-5 Monte Carlo calculations were conducted to test the validity of the expressions. The calculated activity agreed within approximately 1 percent with the literature data used as input in the simulations. PMID:24411317

Sato, Y; Yamada, T; Hasegawa, T

2014-05-01

191

O Teatro Brasileiro na Década de Oitenta  

E-print Network

Buenos Aires. O crepúsculo da década de setenta e o raiar dos anos oitenta também testemunharam as primeiras edições e/ou encenações de vários tratamentos dramáticos de um dos pilares da ditadura-a tortura. Milagre na cela, de Jorge Andrade, estreou... década de oitenta explica-se, ao menos em parte, pelas experiências dos que foram mais de perto atingidos pela censura da ditadura. Com a liberalização política, Boal voltou ao Brasil pela primeira vez em oito anos para dar palestras e dirigir...

Albuquerque, Severino Joã o

1992-04-01

192

High [Na+]i in cardiomyocytes from rainbow trout.  

PubMed

Intracellular Na(+)-concentration, [Na(+)](i) modulates excitation-contraction coupling of cardiac myocytes via the Na(+)/Ca(2+) exchanger (NCX). In cardiomyocytes from rainbow trout (Oncorhyncus mykiss), whole cell patch-clamp studies have shown that Ca(2+) influx via reverse-mode NCX contributes significantly to contraction when [Na(+)](i) is 16 mM but not 10 mM. However, physiological [Na(+)](i) has never been measured. We recorded [Na(+)](i) using the fluorescent indicator sodium-binding benzofuran isophthalate in freshly isolated atrial and ventricular myocytes from rainbow trout. We examined [Na(+)](i) at rest and during increases in contraction frequency across three temperatures that span those trout experience in nature (7, 14, and 21 degrees C). Surprisingly, we found that [Na(+)](i) was not different between atrial and ventricular cells. Furthermore, acute temperature changes did not affect [Na(+)](i) in resting cells. Thus, we report a resting in vivo [Na(+)](i) of 13.4 mM for rainbow trout cardiomyocytes. [Na(+)](i) increased from rest with increases in contraction frequency by 3.2, 4.7, and 6.5% at 0.2, 0.5, and 0.8 Hz, respectively. This corresponds to an increase of 0.4, 0.6, and 0.9 mM at 0.2, 0.5, and 0.8 Hz, respectively. Acute temperature change did not significantly affect the contraction-induced increase in [Na(+)](i). Our results provide the first measurement of [Na(+)](i) in rainbow trout cardiomyocytes. This surprisingly high [Na(+)](i) is likely to result in physiologically significant Ca(2+) influx via reverse-mode NCX during excitation-contraction coupling. We calculate that this Ca(2+)-source will decrease with the action potential duration as temperature and contraction frequency increases. PMID:17507438

Birkedal, Rikke; Shiels, Holly A

2007-08-01

193

Use of ²³Na-nuclear magnetic resonance to follow sodium uptake and efflux in NaCl-adapted and nonadapted millet (Panicum miliaceum) suspensions  

Microsoft Academic Search

Cellular Na\\/sup +\\/ transport was followed in vivo by ²³Na nuclear magnetic resonance (NMR) using anionic dysprosium-based shift reagents to resolve internal and external ²³Na\\/sup +\\/ resonances. Proso millet (Panicum miliaceum) cell suspensions adapted for rapid growth on 130 mM NaCl had biphasic ²³Na efflux kinetics when shifted to low Na..mu.. medium, while nonadapted cells had little measurable Na\\/sup +\\/

L. O. Sillerud; J. W. Heyser

1984-01-01

194

Na? transport in the normal and failing heart - remember the balance.  

PubMed

In the heart, intracellular Na(+) concentration ([Na(+)]i) is a key modulator of Ca(2+) cycling, contractility and cardiac myocyte metabolism. Several Na(+) transporters are electrogenic, thus they both contribute to shaping the cardiac action potential and at the same time are affected by it. [Na(+)]i is controlled by the balance between Na(+) influx through various pathways, including the Na(+)/Ca(2+) exchanger and Na(+) channels, and Na(+) extrusion via the Na(+)/K(+)-ATPase. [Na(+)]i is elevated in HF due to a combination of increased entry through Na(+) channels and/or Na(+)/H(+) exchanger and reduced activity of the Na(+)/K(+)-ATPase. Here we review the major Na(+) transport pathways in cardiac myocytes and how they participate in regulating [Na(+)]i in normal and failing hearts. This article is part of a Special Issue entitled "Na(+) Regulation in Cardiac Myocytes." PMID:23608603

Despa, Sanda; Bers, Donald M

2013-08-01

195

Neutron Multiplicity Discrimination in MoNA  

NASA Astrophysics Data System (ADS)

The Modular Neutron Array (MoNA) is a high-efficiency neutron detector at the National Superconducting Cyclotron Laboratory at MSU, used in conjunction with the NSCL/FSU sweeper magnet to investigate the loss of one or more neutrons from particle-unbound nuclei near and beyond the neutron drip-line. In order to properly analyze data from these experiments it is important to distinguish neutron multiplicity in MoNA. We've developed an algorithm that produces scatter plots of neutron velocity change vs. scattering angle and energy deposition vs. scattering angle, each of which results in a locus of events corresponding (largely) to single neutron multiple-scatter events, and each of which can be used to gate the other for cross-correlation. Challenges to accurate neutron trajectory mapping include sub-threshold neutron scattering from carbon (which changes neutron trajectories in unpredictable ways) and the discrete nature of the array, which (especially for shorter scattering lengths) results in scattering angles not necessarily reflective of actual neutron trajectories. Results for experiments involving one and two neutron decays will be presented.

Rogers, W. F.; Gillette, J.; Gardner, M.; Reed, A.; Mosby, S.

2007-10-01

196

Luminescence of NaCl:Tl and NaCl:In at high pressure  

SciTech Connect

The effects of pressure up to 40 kbar and temperatures down to 100 /sup 0/K on the intensity and lifetime of NaCa:Tl/sup +/ and NaCl:In has been studied. A kinetic model describing these luminescent properties has been proposed. The /sup 3/T/sub lu/ level is in thermal equilibrium with /sup 3/A/sub lu/. Both /sup 3/T/sub lu/ and /sup 3/A/sub lu/ have nonradiative pathways to the ground state /sup 1/A/sub lg/. In addition /sup 3/T/sub lu/ is radiatively coupled to the ground state. Increasing pressure increases the radiative rate from /sup 3/T/sub lu/ to /sup 1/A/sub lg/ and has no effect on the equilibrium between /sup 3/T/sub lu/ and /sup 3/A/sub lu/. The back feeding rate from /sup 3/A/sub lu/ to /sup 3/T/sub lu/ is temperature sensitive for NaCl:Tl but not for NaCl:In.

Littman, J.E.; Drickamer, H.G.

1982-03-01

197

Theoretical calculation of low-lying states of NaAr and NaXe  

NASA Technical Reports Server (NTRS)

Potential curves as well as dipole moments and linking transition moments are calculated for the ground X 2 Sigma + and low lying excited A 2 Pi, B 2 Sigma +, C 2 Sigma +, (4) 2 Sigma +, (2) 2 Pi and (1) 2 Delta states of NaAr and NaXe. Calculations are performed using a self-consistent field plus configuration-interaction procedure with the core electrons replaced by an ab initio effective core potential. The potential curves obtained are found to be considerably less repulsive than the semiempirical curves of Pascale and Vandeplanque (1974) and to agree well with existing experimental data, although the binding energies of those states having potential minima due to van der Waals interactions are underestimated. Emission bands are also calculated for the X 2 Sigma + - C 2 Sigma + excimer transitions of NaAr and NaXe using the calculated transition moments and potential curves, and shown to agree well with experiment on the short-wavelength side of the maximum.

Laskowski, B. C.; Langhoff, S. R.; Stallcop, J. R.

1981-01-01

198

Na-O Anticorrelation And HB I. The Na-O anticorrelation in NGC 2808  

E-print Network

We derived atmospheric parameters and elemental abundances of Fe, O and Na for about 120 red giant stars in the Galactic globular cluster NGC 2808. Our results are based on the analysis of medium-high resolution (R=22000-24000) GIRAFFE spectra acquired with the FLAMES spectrograph at VLT-UT2 as a part of a project aimed at studying the Na-O anticorrelation as a function of physical parameters in globular clusters. We present here the anticorrelation of Na and O abundances in NGC 2808, and we discuss the distribution function of stars along this relation. Besides a bulk of O-normal stars, with composition typical of field halo stars, NGC 2808 seems to host two other groups of O-poor and super O-poor stars. In this regard, NGC 2808 is similar to M 13, the template cluster for the Na-O anticorrelation. However, at variance with M 13, most stars in NGC 2808 are O-rich. This might be related to the horizontal branch morphologies which are very different in these two clusters. The average metallicity we found for NGC 2808 is [Fe/H]=-1.10 (rms=0.065 dex, from 123 stars). We also found some evidence of a small intrinsic spread in metallicity, but more definitive conclusions are hampered by the presence of a small differential reddening.

Eugenio Carretta; Angela Bragaglia; Raffaele Gratton; Francesco Leone; Alejandra Recio-Blanco; Sara Lucatello

2005-11-30

199

Na-O anticorrelation and HB. I. The Na-O anticorrelation in NGC 2808  

NASA Astrophysics Data System (ADS)

We derived the atmospheric parameters and elemental abundances of Fe, O, and Na for about 120 red giant stars in the Galactic globular cluster NGC 2808. Our results are based on the analysis of medium-high resolution (R=22 000{-}24 000) GIRAFFE spectra acquired with the FLAMES spectrograph at VLT-UT2 as a part of a project aimed at studying the Na-O anticorrelation as a function of physical parameters in globular clusters. We present the anticorrelation of Na and O abundances in NGC 2808 here, and discuss the distribution function of stars along this relation. Besides a bulk of O-normal stars with the typical composition of field halo stars, NGC 2808 seems to host two other groups of O-poor and super O-poor stars. In this regard, NGC 2808 is similar to M 13, the template cluster for the Na-O anticorrelation. However, in contrast to M 13, most stars in NGC 2808 are O-rich. This might be related to the horizontal branch morphologies that are very different in these two clusters. The average metallicity we found for NGC 2808 is [Fe/H]=-1.10 (rms = 0.065 dex, from 123 stars). We also found some evidence of a small intrinsic spread in metallicity, but more definitive conclusions are hampered by the presence of a small differential reddening.

Carretta, E.; Bragaglia, A.; Gratton, R. G.; Leone, F.; Recio-Blanco, A.; Lucatello, S.

2006-05-01

200

Minimizing Load Effects on NA4 Gear Vibration Diagnostic Parameter  

NASA Technical Reports Server (NTRS)

NA4 is a vibration diagnostic parameter, developed by researchers at NASA Glenn Research Center, for health monitoring of gears in helicopter transmissions. The NA4 reacts to the onset of gear pitting damage and continues to react to the damage as it spreads. This research also indicates NA4 reacts similarly to load variations. The sensitivity of NA4 to load changes will substantially affect its performance on a helicopter gearbox that experiences continuously changing load throughout its flight regimes. The parameter NA4 has been used to monitor gear fatigue tests at constant load. At constant load, NA4 effectively detects the onset of pitting damage and tracks damage severity. Previous research also shows that NA4 reacts to changes in load applied to the gears in the same way it reacts to the onset of pitting damage. The method used to calculate NA4 was modified to minimize these load effects. The modified NA4 parameter was applied to four sets of experimental data. Results indicate the modified NA4 is no longer sensitive to load changes, but remains sensitive to pitting damage.

Dempsey, Paula J.; Zakrajsek, James J.

2001-01-01

201

NA Nonlinear Equation-of-state Inversion  

NASA Astrophysics Data System (ADS)

A fully non-linear inversion scheme is introduced for the determination of the parameters controlling the equation-of-state and elasticity of mineral phases using the thermodynamically consistent finite-strain formulation introduced by Stixrude & Lithgow-Bertelloni (2005). This inversion exploits a directed search in an eight-dimensional parameter space using the Neighbourhood Algorithm (NA) of Sambridge (1999) to search for the minimum of an objective function representing the misfit to multiple data sets that constrain different aspects of the mineral behaviour. No derivatives are employed and the progress towards the minimum builds on the accumulated information on the character of the parameter space acquired as the inversion progresses. When only a limited range of experimental information is available there is a strong possibility of multiple minima in the objective function, which can pose problems for conventional iterative least-squares or other gradient methods. The addition of many different styles of data tends to produce a better defined minimum. The influence of different data types can be readily assessed by allowing differential weighting. The new procedure is illustrated by application to MgO, for which extensive experimental data are available. These include the variation of relative volume V with temperature T and pressure P from both static and shock-compression experiments, acoustic measurements of compressional and shear (and hence bulk) moduli, and calorimetric determinations of entropy as a function of temperature at atmospheric pressure. Preliminary NA modeling highlighted tensions between marginally incompatible subsets of data. We therefore excluded one-atmosphere V(T) data for T ? 1800 K for which the quasi-harmonic approximation is inadequate (Wu et al., 2008) along with elastic moduli derived from Brillouin spectroscopy under conditions (P ? 14 GPa) where significant departures from hydrostatic conditions are expected. With these limited exclusions based on sound physical principles, the NA search identified a compact family of models that provide an excellent fit to the diverse experimental data and a measure of the covariance between key model parameters.

Jackson, I.; Kennett, B. L.

2008-12-01

202

Na?La?(CO?)? and CsNa?Ca?(CO?)?: two new carbonates as UV nonlinear optical materials.  

PubMed

Two nonlinear optical crystal carbonates (Na4La2(CO3)5 and CsNa5Ca5(CO3)8 were successfully synthesized by hydrothermal method, and both of them crystallized in the same noncentrosymmetric hexagonal space group P63mc (No. 186). The structure of Na4La2(CO3)5 consists of a three-dimensional network made up of [CO3] triangles as well as irregular [Na(0.67)La(0.33)O10] and [NaO8] polyhedra. The structure of CsNa5Ca5(CO3)8 can be described as the standing-on-edge [CO3] groups connect the adjacent infinite [CaCO3]? layers in the ab plane to construct a framework with four types of channels running parallel to [010]. The Na, Cs, and [Na(0.67)Ca(0.33)] atoms reside in these channels. The measurement of second harmonic generation (SHG) by the method adapted from Kurtz and Perry indicated that Na4La2(CO3)5 and CsNa5Ca5(CO3)8 were phase-matchable in the visible region and exhibited SHG responses of approximately 3 and 1 × KH2PO4 (KDP). Meanwhile, they exhibited wide transparent region with short UV cutoff edge at about 235 and 210 nm, respectively, suggesting that these crystals as NLO materials may have potential applications in the UV region. PMID:25027762

Luo, Min; Wang, GenXiang; Lin, Chensheng; Ye, Ning; Zhou, Yuqiao; Cheng, Wendan

2014-08-01

203

Ionic Liquid Electrolytes with Various Sodium Solutes for Rechargeable Na/NaFePO4 Batteries Operated at Elevated Temperatures.  

PubMed

NaFePO4 with an olivine structure is synthesized via chemical delithiation of LiFePO4 followed by electrochemical sodiation of FePO4. Butylmethylpyrrolidinium-bis(trifluoromethanesulfonyl)imide (BMP-TFSI) ionic liquid (IL) with various sodium solutes, namely NaBF4, NaClO4, NaPF6, and NaN(CN)2, is used as an electrolyte for rechargeable Na/NaFePO4 cells. The IL electrolytes show high thermal stability (>350 °C) and nonflammability, and are thus ideal for high-safety applications. The highest conductivity and the lowest viscosity of the electrolyte are obtained with NaBF4. At an elevated temperature (above 50 °C), the IL electrolyte is more suitable than a conventional organic electrolyte for the sodium cell. At 75 °C, the measured capacity of NaFePO4 in a NaBF4-incorporated IL electrolyte is as high as 152 mAh g(-1) (at 0.05 C), which is near the theoretical value (154 mAh g(-1)). Moreover, 60% of this capacity can be retained when the charge-discharge rate is increased to 1 C. PMID:25295391

Wongittharom, Nithinai; Wang, Chueh-Han; Wang, Yi-Chen; Yang, Cheng-Hsien; Chang, Jeng-Kuei

2014-10-22

204

Pyrophosphate-Fueled Na+ and H+ Transport in Prokaryotes  

PubMed Central

SUMMARY In its early history, life appeared to depend on pyrophosphate rather than ATP as the source of energy. Ancient membrane pyrophosphatases that couple pyrophosphate hydrolysis to active H+ transport across biological membranes (H+-pyrophosphatases) have long been known in prokaryotes, plants, and protists. Recent studies have identified two evolutionarily related and widespread prokaryotic relics that can pump Na+ (Na+-pyrophosphatase) or both Na+ and H+ (Na+,H+-pyrophosphatase). Both these transporters require Na+ for pyrophosphate hydrolysis and are further activated by K+. The determination of the three-dimensional structures of H+- and Na+-pyrophosphatases has been another recent breakthrough in the studies of these cation pumps. Structural and functional studies have highlighted the major determinants of the cation specificities of membrane pyrophosphatases and their potential use in constructing transgenic stress-resistant organisms. PMID:23699258

Malinen, Anssi M.; Luoto, Heidi H.

2013-01-01

205

Na+ transport in the normal and failing heart - remember the balance  

PubMed Central

In the heart, intracellular Na+ concentration ([Na+]i) is a key modulator of Ca2+ cycling, contractility and cardiac myocyte metabolism. Several Na+ transporters are electrogenic, thus they both contribute to shaping the cardiac action potential and at the same time are affected by it. [Na+]i is controlled by the balance between Na+ influx through various pathways, including the Na+/Ca2+ exchanger and Na+ channels, and Na+ extrusion via the Na+/K+-ATPase. [Na+]i is elevated in HF due to a combination of increased entry through Na+ channels and/or Na+/H+ exchanger and reduced activity of the Na+/K+-ATPase. Here we review the major Na+ transport pathways in cardiac myocytes and how they participate in regulating [Na+]i in normal and failing hearts. PMID:23608603

Despa, Sanda; Bers, Donald M.

2013-01-01

206

Cosmic Muon Tracking with MoNA  

NASA Astrophysics Data System (ADS)

The Modular Neutron Array (MoNA) consists of 144 2-meter long scintillating bar detectors arranged in a structure 9 bars deep and 16 bars high. Each detector has a photo-multiplier tube attached at each end, allowing for position determination of events based on timing difference. The array is housed at the National Superconducting Cyclotron Laboratory at Michigan State University and will be used to study exotic nuclei near the neutron dripline. The array is tested and calibrated using cosmic muons, and an algorithm has been developed to extract high multiplicity muon events from the datastream to enable precise tracking for use in position calibration of the detectors. This method represents a superior calibration procedure since in addition to correcting for differential time response between the PMTs at the ends of each bar, it also accounts for any relative physical misalignment of the detectors in the array.

Watters, Kyle; Elliott, Lance; Strongman, Michael; Rogers, Warren

2004-10-01

207

Lakota na Dakota Wowapi Oti Kin  

NSDL National Science Digital Library

The Lakota na Dakota Wowapi Oti Kin is a one-stop information portal containing Internet resources on people of Lakota and Dakota descent (also known as Sioux or Siouan peoples). Created and maintained by Professor Martin Brokenleg of Augustana College and Dr. Raymond Bucko, S.J. of Creighton University, this site offers Web links in various categories including art and artists / music and musicians, bibliographic resources, demography, education, history, language resources, legal issues, maps, museums, and a host of other Lakota-related sites. For those interested in locating information in the field of Native American studies, specifically on the Sioux peoples, this site is an excellent place to begin your search.

208

Function of FXYD Proteins, Regulators of Na, K-ATPase  

Microsoft Academic Search

   In this short review, we summarize our work on the role of members of the FXYD protein family as tissue-specific modulators\\u000a of Na, K-ATPase. FXYD1 or phospholemman, mainly expressed in heart and skeletal muscle increases the apparent affinity for\\u000a intracellular Na+ of Na, K-ATPase and may thus be important for appropriate muscle contractility. FXYD2 or ? subunit and FXYD4 or

Käthi Geering

2005-01-01

209

Human myocardial Na,K-ATPase concentration in heart failure  

Microsoft Academic Search

The Na,K-ATPase is of major importance for active ion transport across the sarcolemma and thus for electrical as well as contractile function of the myocardium. Furthermore, it is receptor for digitalis glycosides. In human studies of the regulatory aspects of myocardial Na,K-ATPase concentration a major problem has been to obtain tissue samples. Methodological accomplishments in quantification of myocardial Na,K-ATPase using

Henning Bundgaard; Keld Kjeldsen

1996-01-01

210

Sodium superionic conduction in Na2B12H12.  

PubMed

Impedance measurements indicate that Na2B12H12 exhibits dramatic Na(+) conductivity (on the order of 0.1 S cm(-1)) above its order-disorder phase-transition at ?529 K, rivaling that of current, solid-state, ceramic-based, Na-battery electrolytes. Superionicity may be aided by the large size, quasispherical shape, and high rotational mobility of the B12H12(2-) anions. PMID:24584582

Udovic, Terrence J; Matsuo, Motoaki; Unemoto, Atsushi; Verdal, Nina; Stavila, Vitalie; Skripov, Alexander V; Rush, John J; Takamura, Hitoshi; Orimo, Shin-ichi

2014-04-11

211

Magnesium correction to the NaKCa chemical geothermometer  

USGS Publications Warehouse

Equations and graphs have been devised to correct for the adverse effects of magnesium upon the Na-K-Ca chemical geothermometer. Either the equations or graphs can be used to determine appropriate temperature corrections for given waters with calculated NaKCa temperatures > 70??C and R 50 are probably derived from relatively cool aquifers with temperatures approximately equal to the measured spring temperature, irrespective of much higher calculated Na-K-Ca temperatures. ?? 1979.

Fournier, R.O.; Potter, R.W., II

1979-01-01

212

Na+ interactions with the neutral amino acid transporter ASCT1.  

PubMed

The alanine, serine, cysteine transporters (ASCTs) belong to the solute carrier family 1A (SLC1A), which also includes the excitatory amino acid transporters (EAATs) and the prokaryotic aspartate transporter GltPh. Acidic amino acid transport by the EAATs is coupled to the co-transport of three Na(+) ions and one proton, and the counter-transport of one K(+) ion. In contrast, neutral amino acid exchange by the ASCTs does not require protons or the counter-transport of K(+) ions and the number of Na(+) ions required is not well established. One property common to SLC1A family members is a substrate-activated anion conductance. We have investigated the number and location of Na(+) ions required by ASCT1 by mutating residues in ASCT1 that correspond to residues in the EAATs and GltPh that are involved in Na(+) binding. Mutations to all three proposed Na(+) sites influence the binding of substrate and/or Na(+), or the rate of substrate exchange. A G422S mutation near the Na2 site reduced Na(+) affinity, without affecting the rate of exchange. D467T and D467A mutations in the Na1 site reduce Na(+) and substrate affinity and also the rate of substrate exchange. T124A and D380A mutations in the Na3 site selectively reduce the affinity for Na(+) and the rate of substrate exchange without affecting substrate affinity. In many of the mutants that reduce the rate of substrate transport the amplitudes of the substrate-activated anion conductances are not substantially affected indicating altered ion dependence for channel activation compared with substrate exchange. PMID:24808181

Scopelliti, Amanda J; Heinzelmann, Germano; Kuyucak, Serdar; Ryan, Renae M; Vandenberg, Robert J

2014-06-20

213

Kinetics of 9-aminoacridine block of single Na channels  

Microsoft Academic Search

The kinetics of 9-aminoacridine (9-AA) block of single Na chan- nels in neuroblastoma N 1 E-115 cells were studied using the gigohm seal, patch clamp technique, under the condition in which the Na current inactivation had been eliminated by treatment with N-bromoacetamide (NBA) . Following NBA treatment, the current flowing through individual Na channels was manifested by square-wave open events

DAISUKE YAMAMOTO; JAY Z. YEH

1984-01-01

214

CaMKII-dependent regulation of cardiac Na+ homeostasis  

PubMed Central

Na+ homeostasis is a key regulator of cardiac excitation and contraction. The cardiac voltage-gated Na+ channel, NaV1.5, critically controls cell excitability, and altered channel gating has been implicated in both inherited and acquired arrhythmias. Ca2+/calmodulin-dependent protein kinase II (CaMKII), a serine/threonine kinase important in cardiac physiology and disease, phosphorylates NaV1.5 at multiple sites within the first intracellular linker loop to regulate channel gating. Although CaMKII sites on the channel have been identified (S516, T594, S571), the relative role of each of these phospho-sites in channel gating properties remains unclear, whereby both loss-of-function (reduced availability) and gain-of-function (late Na+ current, INaL) effects have been reported. Our review highlights investigating the complex multi-site phospho-regulation of NaV1.5 gating is crucial to understanding the genesis of acquired arrhythmias in heart failure (HF) and CaMKII activated conditions. In addition, the increased Na+ influx accompanying INaL may also indirectly contribute to arrhythmia by promoting Ca2+ overload. While the precise mechanisms of Na+ loading during HF remain unclear, and quantitative analyses of the contribution of INaL are lacking, disrupted Na+ homeostasis is a consistent feature of HF. Computational and experimental observations suggest that both increased diastolic Na+ influx and action potential prolongation due to systolic INaL contribute to disruption of Ca2+ handling in failing hearts. Furthermore, simulations reveal a synergistic interaction between perturbed Na+ fluxes and CaMKII, and confirm recent experimental findings of an arrhythmogenic feedback loop, whereby CaMKII activation is at once a cause and a consequence of Na+ loading. PMID:24653702

Grandi, Eleonora; Herren, Anthony W.

2014-01-01

215

Mercury's Na Exosphere from MESSENGER Data  

NASA Technical Reports Server (NTRS)

MESSENGER entered orbit about Mercury on March 18, 2011. Since then, the Ultraviolet and Visible Spectrometer (UWS) channel of MESSENGER's Mercury Atmospheric and Surface Composition Spectrometer (MASCS) has been observing Mercury's exosphere nearly continuously. Daily measurements of Na brightness were fitted with non-uniform exospheric models. With Monte Carlo sampling we traced the trajectories of a representative number of test particles, generally one million per run per source process, until photoionization, escape from the gravitational well, or permanent sticking at the surface removed the atom from the simulation. Atoms were assumed to partially thermally accommodate on each encounter with the surface with accommodation coefficient 0.25. Runs for different assumed source processes are run separately, scaled and co-added. Once these model results were saved onto a 3D grid, we ran lines of sight from the MESSENGER spacecraft :0 infinity using the SPICE kernels and we computed brightness integrals. Note that only particles that contribute to the measurement can be constrained with our method. Atoms and molecules produced on the nightside must escape the shadow in order to scatter light if the excitation process is resonant-light scattering, as assumed here. The aggregate distribution of Na atoms fits a 1200 K gas, with a PSD distribution, along with a hotter component. Our models constrain the hot component, assumed to be impact vaporization, to be emitted with a 2500 K Maxwellian. Most orbits show a dawnside enhancement in the hot component broadly spread over the leading hemisphere. However, on some dates there is no dawn/dusk asymmetry. The portion of the hot/cold source appears to be highly variable.

Killen, Rosemary M.; Burger, M. H.; Cassidy, T. A.; Sarantos, M.; Vervack, R. J.; McClintock, W. El; Merkel, A. W.; Sprague, A. L.; Solomon, S. C.

2012-01-01

216

Questões mais freqüentes na área de astronomia  

NASA Astrophysics Data System (ADS)

É amplamente reconhecido que as pessoas em geral têm grande fascínio e interesse pela Astronomia. Por outro lado, o conteúdo dessa área incluído no ensino formal está longe de ser abrangente e suficiente para suprir a demanda. Esse interesse permite otimizar a divulgação e o ensino não formal de Astronomia aliando a adequação do conteúdo à expectativa das pessoas. Nesse intuito, nosso trabalho busca mapear que assuntos da Astronomia despertam mais a atenção e o interesse das pessoas e o quanto disso é estimulado pela mídia, que freqüentemente aproveita-se desse interesse de forma sensacionalista, trazendo contribuições positivas e negativas. Para esta avaliação, utilizamos as pesquisas específicas e de caracterização do público que freqüentou o MAst nos anos de 2001 a 2003, pesquisa das matérias de Astronomia veiculadas na mídia escrita da cidade do Rio de Janeiro no mesmo período, além da base de dados do programa Pergunte a um astrônomo, realizado no Observatório Nacional nos anos de 1997 a 1999. Para a análise dos dados, dividimos as perguntas em dez categorias, cada uma com suas subcategorias, tomando como referência as divisões, comissões e grupos de trabalho da IAU. Apresentamos neste trabalho os resultados dessa pesquisa, que incluem, entre outros, a predominância de questões nas categorias Observação do Céu e Sistemas Planetários enquanto nos jornais dominam as notícias de Sistemas Planetários e Espaço & Astronáutica. Outro resultado interessante indicou que as dúvidas de crianças do ensino fundamental se concentram em algumas categorias enquanto as questões de adultos são mais distribuídas por todas as categorias. Os resultados dessa pesquisa serão aplicados diretamente no aperfeiçoamento das atividades do Programa de Observação de Céu, bem como na elaboração de novos projetos e eventos realizados no MAst.

Segundo, H. A. S.; Garcia, G. C.; Caretta, C. A.; Lima, F. P.

2003-08-01

217

Kaolin-based geopolymers with various NaOH concentrations  

NASA Astrophysics Data System (ADS)

Kaolin geopolymers were produced by the alkali-activation of kaolin with an activator solution (a mixture of NaOH and sodium silicate solutions). The NaOH solution was prepared at a concentration of 6-14 mol/L and was mixed with the sodium silicate solution at a Na2SiO3/NaOH mass ratio of 0.24 to prepare an activator solution. The kaolin-to-activator solution mass ratio used was 0.80. This paper aimed to analyze the effect of NaOH concentration on the compressive strength of kaolin geopolymers at 80°C for 1, 2, and 3 d. Kaolin geopolymers were stable in water, and strength results showed that the kaolin binder had adequate compressive strength with 12 mol/L of NaOH concentration. When the NaOH concentration increased, the SiO2/Na2O decreased. The increased Na2O content enhanced the dissolution of kaolin as shown in X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analyses. However, excess in this content was not beneficial for the strength development of kaolin geopolymers. In addition, there was the formation of more geopolymeric gel in 12 mol/L samples. The XRD pattern of the samples showed a higher amorphous content and a more geopolymer bonding existed as proved by FTIR analysis.

Heah, C. Y.; Kamarudin, H.; Mustafa Al Bakri, A. M.; Bnhussain, M.; Luqman, M.; Khairul Nizar, I.; Ruzaidi, C. M.; Liew, Y. M.

2013-03-01

218

Reconstitution of a bacterial Na+/H+ antiporter  

SciTech Connect

Membrane proteins from alkalophilic Bacillus firmus RAB were extracted with octylglucoside, reconstituted into liposomes made from alkalophile lipids. The proteoliposomes were loaded with SSNa . Imposition of a valinomycin-mediated potassium diffusion potential, positive out, resulted in very rapid efflux of radioactive Na against its electrochemical gradient. That the Na efflux was mediated by the electrogenic Na /H antiporter is indicated by the following characteristics that had been established for the porter in previous studies: dependence upon an electrical potential; pH sensitivity, with activity dependent upon an alkaline pH; inhibition by Li ; and an apparent concentration dependence upon Na that correlated well with measurements in cells and membrane vesicles.

Seto-Young, D.; Garcia, M.L.; Krulwich, T.A.

1985-09-25

219

Association reactions of Na(+) and some implications for interstellar chemistry  

NASA Astrophysics Data System (ADS)

Three-body association reactions of Na+ with a variety of neutral gases in the presence of He as third body have been studied in a Selected Ion Flow Tube at 80K. The measured rate coefficients have been utilized to estimate radiative association rate coefficients at 20K. It is shown that Na+-neutral radiative association reactions play a role in the depletion of Na+ in dense interstellar clouds and can produce a sufficient amount of NaOH to explain its observed tentative abundance in Sgr B2.

Smith, D.; Adams, N. G.; Alge, E.; Herbst, E.

1983-09-01

220

Use of Na-Nuclear Magnetic Resonance To Follow Sodium Uptake and Efflux in NaCl-Adapted and Nonadapted Millet (Panicum miliaceum) Suspensions.  

PubMed

Cellular Na(+) transport was followed in vivo by (23)Na nuclear magnetic resonance (NMR) using anionic dysprosium-based shift reagents to resolve internal and external (23)Na(+) resonances. Proso millet (Panicum miliaceum) cell suspensions adapted for rapid growth on 130 mm NaCl had biphasic (23)Na efflux kinetics when shifted to low Na(+) medium, while nonadapted cells had little measurable Na(+) efflux after preloading with (23)NaCl. Uptake of (23)Na was also observed using (23)Na NMR. The resonance frequency of the external Na(+)-dysprosium (III) triphosphate, relative to that of the (23)Na in the cells, was sensitive to pH, permitting the pH of the external medium to be followed during the course of in vivo experiments. PMID:16663592

Sillerud, L O; Heyser, J W

1984-05-01

221

Use of /sup 23/Na-nuclear magnetic resonance to follow sodium uptake and efflux in NaCl-adapted and nonadapted millet (Panicum miliaceum) suspensions  

SciTech Connect

Cellular Na/sup +/ transport was followed in vivo by /sup 23/Na nuclear magnetic resonance (NMR) using anionic dysprosium-based shift reagents to resolve internal and external /sup 23/Na/sup +/ resonances. Proso millet (Panicum miliaceum) cell suspensions adapted for rapid growth on 130 mM NaCl had biphasic /sup 23/Na efflux kinetics when shifted to low Na..mu.. medium, while nonadapted cells had little measurable Na/sup +/ efflux after preloading with /sup 23/NaCl. Uptake of /sup 23/Na was also observed using /sup 23/Na NMR. The resonance frequency of the external Na/sup +/-dysprosium (III) triphosphate, relative to that of the /sup 23/Na in the cells, was sensitive to pH, permitting the pH of the external medium to be followed during the course of in vivo experiments. 20 references, 3 figures.

Sillerud, L.O.; Heyser, J.W.

1984-05-01

222

Electrode potential studies of liquid-solid equilibrium in Na{sub 3}Bi-saturated Na-Bi melts  

SciTech Connect

Liquid binary Na-Bi alloys have been studied extensively because of the interest in possible uses as electrochemically active couples in regenerative electrochemical cells, in high-energy batteries, and as potential heat-transfer media in nuclear reaction processes. Techniques used previously for thermodynamic measurements on the system have included the transpiration method, quasi-static and boiling point methods, the electromotive force (emf) method using molten salt mixtures and various solid electrolytes, and thermal analysis. Most of the thermodynamic data on Bi-Na alloys were recently assessed by Sangster and Pelton. However, no experimental thermodynamic data for liquid-solid equilibrium in liquid Na-Bi alloys saturated with solid Na{sub 3}Bi have been reported in the literature. The aim of the present work was to investigate experimentally the equilibrium between Na-Bi melts and solid Na{sub 3}Bi by the emf method in the range 638 to 973 K.

Yan, X.Y.; Langberg, D.E.; Rankin, W.J.

2000-04-01

223

Mechanism of ?-Conotoxin PIIIA Binding to the Voltage-Gated Na+ Channel NaV1.4  

PubMed Central

Several subtypes of voltage-gated Na+ (NaV) channels are important targets for pain management. ?-Conotoxins isolated from venoms of cone snails are potent and specific blockers of different NaV channel isoforms. The inhibitory effect of ?-conotoxins on NaV channels has been examined extensively, but the mechanism of toxin specificity has not been understood in detail. Here the known structure of ?-conotoxin PIIIA and a model of the skeletal muscle channel NaV1.4 are used to elucidate elements that contribute to the structural basis of ?-conotoxin binding and specificity. The model of NaV1.4 is constructed based on the crystal structure of the bacterial NaV channel, NaVAb. Six different binding modes, in which the side chain of each of the basic residues carried by the toxin protrudes into the selectivity filter of NaV1.4, are examined in atomic detail using molecular dynamics simulations with explicit solvent. The dissociation constants (Kd) computed for two selected binding modes in which Lys9 or Arg14 from the toxin protrudes into the filter of the channel are within 2 fold; both values in close proximity to those determined from dose response data for the block of NaV currents. To explore the mechanism of PIIIA specificity, a double mutant of NaV1.4 mimicking NaV channels resistant to ?-conotoxins and tetrodotoxin is constructed and the binding of PIIIA to this mutant channel examined. The double mutation causes the affinity of PIIIA to reduce by two orders of magnitude. PMID:24676211

Chen, Rong; Robinson, Anna; Chung, Shin-Ho

2014-01-01

224

Enhancement of the sulfur capture capacity of limestones by the addition of Na2CO3 and NaCl.  

PubMed

The ability of Na2CO3 and NaCl to enhance the sulfur capture capacity of three limestones was evaluated via fixed-bed calcination and sulfation experiments. The tested limestones represent three different sulfation morphologies: unreacted-core, network, and uniformly sulfated. Treatment with aqueous or powdered Na2CO3 significantly increased the Ca-utilization for two stones which normally sulfate in an unreacted-core pattern (20% to 45%) and network pattern (33% to 49%). The increase was lower for the uniformly sulfated stone (44% to 48%). Na2CO3 treatment increased the number of macropores leading to uniform sulfation of all particles, nearly eliminating the normal strong dependence of utilization on limestone type and particle size. The effect of Na2CO3 is believed to be associated with formation of a eutectic melt which enhances ionic diffusion and accelerates molecular rearrangement of the CaO. Treatment with aqueous NaCl solution caused a decrease in utilization, probably due to formation of large grains and plugging of pores caused by formation of a large amount of eutectic melt. The effect of Na2CO3 is less sensitive than that of NaCl to the amount added and the combustion environment (temperature and gas composition). In addition, Na2CO3 neither promotes corrosion nor forms chlorinated byproducts, which are main concerns associated with NaCl. Thus, Na2CO3 appears to have significant advantages over NaCl for enhancement of limestone sulfur capture capacity in fluidized-bed combustors. PMID:11718362

Laursen, K; Grace, J R; Lim, C J

2001-11-01

225

RNA adducts with Na 2SeO 4 and Na 2SeO 3 - Stability and structural features  

NASA Astrophysics Data System (ADS)

Selenium compounds are widely available in dietary supplements and have been extensively studied for their antioxidant and anticancer properties. Low blood Se levels were found to be associated with an increased incidence and mortality from various types of cancers. Although many in vivo and clinical trials have been conducted using these compounds, their biochemical and chemical mechanisms of efficacy are the focus of much current research. This study was designed to examine the interaction of Na 2SeO 4 and Na 2SeO 3 with RNA in aqueous solution at physiological conditions, using a constant RNA concentration (6.25 mM) and various sodium selenate and sodium selenite/polynucleotide (phosphate) ratios of 1/80, 1/40, 1/20, 1/10, 1/5, 1/2 and 1/1. Fourier transform infrared, UV-Visible spectroscopic methods were used to determine the drug binding modes, the binding constants, and the stability of Na 2SeO 4 and Na 2SeO 3-RNA complexes in aqueous solution. Spectroscopic evidence showed that Na 2SeO 4 and Na 2SeO 3 bind to the major and minor grooves of RNA ( via G, A and U bases) with some degree of the Se-phosphate (PO 2) interaction for both compounds with overall binding constants of K(Na 2SeO 4-RNA) = 8.34 × 10 3 and K(Na 2SeO 3-RNA) = 4.57 × 10 3 M -1. The order of selenium salts-biopolymer stability was Na 2SeO 4-RNA > Na 2SeO 3-RNA. RNA aggregations occurred at higher selenium concentrations. No biopolymer conformational changes were observed upon Na 2SeO 4 and Na 2SeO 3 interactions, while RNA remains in the A-family structure.

Nafisi, Shohreh; Manouchehri, Firouzeh; Montazeri, Maryam

2011-12-01

226

Conductance and gating of epithelial Na channels from rat cortical collecting tubule. Effects of luminal Na and Li  

Microsoft Academic Search

The behavior of individual Na channels in the apical membrane of the rat cortical collecting tubule (CCT) was studied at different concentrations of the permeant ions Na and Li. Tubules were opened to expose their luminal sur- faces and bathed in K-gluconate medium to minimize tubule-to-tubule variation in cell membrane potential and intracellular Na concentration. The patch-clamp technique was used

LAWRENCE G. PALMER; GUSTAVO FRINDT

1988-01-01

227

Crnica I Encontro Solar na UDC O pasado mrcores 24 de abril tivo lugar na Praza da Fraga (Campus da  

E-print Network

/informativos/os-universitarios-da-coruna-comparten-unha-xornada-de- gastronomia-solar-573783 http://www.lavozdegalicia.es/noticia/coruna/2013/04/24/cocina-universitaria-energiaCr�nica I Encontro Solar na UDC O pasado m�rcores 24 de abril tivo lugar na Praza da Fraga (Campus da Zapateira) a primeira edici�n do Encontro Solar na UDC. Esta era a terceira das actividades

Fraguela, Basilio B.

228

Solubility of MgO in MgCl{sub 2}-NaCl-NaF melts  

SciTech Connect

The solubility of MgO in MgCl{sub 2}-NaCl-NaF melts has been measured for melts with varying NaF concentration for x{sub MgCl{sub 2}} = 0.10 and 0.63 and for x{sub MgCl{sub 2}}/x{sub NaCl} = 1.70. Melt samples have been analyzed by carbothermal reduction (Leco TC-436) for total oxide content. The oxide content in the binary melt MgCl{sub 2}-NaCl was also analyzed by Iodometric titration. The results indicate two different oxide-containing species, denoted MgOCl and MgOF, in the solidified samples withdrawn from the melt. The latter appears only in fluoride-containing melts, but may, however, also contain chloride ions. The oxide solubility is increasing with increasing concentration of Mg{sup 2+} in both MgCl{sub 2}-NaCl and MgCl{sub 2}-NaCl-NaF melts. The solubility of MgO is always higher in fluoride containing melts at the same Mg{sup 2+} concentration. In the systems concentrated in MgCl{sub 2}, the increase in oxide solubility as function of x{sub NaF} is more pronounced than what is predicted from a simple model calculation. The increase is much smaller in the x{sub NaF} < 0.2 range for small MgCl{sub 2} contents than predicted from the same model calculation. The introduction of 1.7 mol% NaF to an industrial electrolyte does not seem to change the oxide solubility significantly. In such an electrolyte, where x{sub MgCl{sub 2}} {approx_equal} 0.1, the data gives a constant oxide solubility around 10 ppm O up to 5 mol% NaF.

Mediaas, H.; Vinstad, J.E.; Oestvold, T. [Norwegian Inst. of Tech., Trondheim (Norway). Inst. of Inorganic Chemistry

1996-10-01

229

Propagac ~ao acustica em aguas profundas na presenca de massas d'aguas de mesoescala na costa sudoeste de Portugal  

E-print Network

Propagac¸ ~ao ac´ustica em ´aguas profundas na presenc¸a de massas d'´aguas de mesoescala na costa trabalho baseia-se no estudo da propagac¸ ~ao sonora em ´aguas oce^anicas profundas na costa Portuguesa massa d'´agua de mesosescala. Mostraremos que tal massa d'´agua d´a origem `a um acoplamento entre dois

Jesus, Sérgio M.

230

EDITORIAL: TaCoNa-Photonics 2008 TaCoNa-Photonics 2008  

Microsoft Academic Search

This special section on theoretical and computational nano-photonics features papers presented at the first International Workshop on Theoretical and Computational Nano-Photonics (TaCoNa-Photonics 2008) held in Bad Honnef, Germany, 3-5 December 2008. The workshop covered a broad range of topics related to current developments and achievements in this interdisciplinary area of research. Since the late 1960s, the word `photonics' has been

Dmitry N. Chigrin; Kurt Busch; Andrei V. Lavrinenko

2009-01-01

231

Central role of the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) in sodium bioenergetics of Vibrio cholerae.  

PubMed

Abstract Vibrio cholerae is a Gram-negative bacterium that lives in brackish or sea water environments. Strains of V. cholerae carrying the pathogenicity islands infect the human gut and cause the fatal disease cholera. Vibrio cholerae maintains a Na+ gradient at its cytoplasmic membrane that drives substrate uptake, motility, and efflux of antibiotics. Here, we summarize the major Na+-dependent transport processes and describe the central role of the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR), a primary Na+ pump, in maintaining a Na+-motive force. The Na+-NQR is a membrane protein complex with a mass of about 220 kDa that couples the exergonic oxidation of NADH to the transport of Na+ across the cytoplasmic membrane. We describe the molecular architecture of this respiratory complex and summarize the findings how electron transport might be coupled to Na+-translocation. Moreover, recent advances in the determination of the three-dimensional structure of this complex are reported. PMID:25205724

Steuber, Julia; Halang, Petra; Vorburger, Thomas; Steffen, Wojtek; Vohl, Georg; Fritz, Günter

2014-12-01

232

Estimating the hydrogen ion concentration in concentrated NaCl and Na{sub 2}SO{sub 4} electrolytes  

SciTech Connect

Combination glass electrodes were tested for determining H{sup +} concentrations in concentrated pure and mixed NaCl and Na{sub 2}SO{sub 4} solutions, as well as natural brine systems. NaCl, Na{sub 2}SO{sub 4}, and mixtures of NaCl and Na{sub 2}SO{sub 4} solutions were analyzed. Correction factors for estimating pC{sub H}{sup +} (negative logarithm of H{sup +} concentration) were determined from measured/observed pH values. Required Gran-type titrations were done with HCl and/or NaOH. The titration method is described and a step-by-step procedure provided; it has been used previously for determining pC{sub H}{sup +} values of synthetic chloride-dominated brines. Precautions are required to determine correction factors for electrolytes that react with H{sup +} or OH{sup {minus}} [sulfate brines for titration with acid; magnesium brines for titration with base because of precipitation of Mg(OH)2]. Correction factors A (pC{sub H}{sup +} = pH{sub ob} + A) from HCl titrations were similar to those from NaOH titrations where the concentration of free H{sup +} was calculated using a thermodynamic model. These values should be applicable to solns with a very large range in measured pH values (2 to 12). Because a large number of solns were titrated with HCl and the A values are similar for HCl and NaOH titrations, the A values for NaCl and Na2SO4 solns were fit as a function of molality to allow extrapolation. For NaCl solns 0 to 6.0 M, A can be obtained by multiplying the molality by 0.159. For Na2SO4 solns 0 to 2.0 M, the values of A can be obtained from (0.221 {minus} 0.549X + 0.201X{sup 2}), where X is the molality of Na{sub 2}SO{sub 4}. Orion-Ross electrode evaluations indicated that the A values did not differ significantly for different electrodes. Results suggest that the data in this report can be used to estimate A values for different NaCl and Na{sub 2}SO{sub 4} solns even for noncalibrated electrodes.

Rai, D.; Felmy, A.R.; Juracich, S.P.; Rao, F. [Pacific Northwest Lab., Richland, WA (United States)

1995-06-01

233

Names of God and Exodus 3:16 Je rey D. Oldham  

E-print Network

, to witness, be gracious saying Jacob and Isaac Abraham of God the me unto appeared has '^ab your father, chief, ruler; lord, master, teacher; advisor, counsellor of God the Jehovah them unto say and Israel the elders of Israel together, and say unto them, Jehovah the God of your fathers, the God of Abraham, Isaac

Pratt, Vaughan

234

Gradiance On-Line Learning Lab Users' Manual Je rey D. Ullman  

E-print Network

problems, and programming labs in SQL, particularly, how one assembles questions into homeworks, and how one issues assignments to a class. Contents 1 Root Questions 2 1.1 Motivation for Root Questions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 3 Creating Homeworks 6 3.1 Creating a New Homework

Pratt, Vaughan

235

Molecular Identification of the Fall Armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera  

E-print Network

1986, Lu & Adang 1996). In Arkansas, estimates of cotton losses alone from fall armyworm damage were $2 most important pest to cotton in Arkansas (Williams 2003). The preferred host plants of FAW came under in Arkansas. A 611-bp region of the mtDNA COI, COII genes was amplified using PCR and then sequenced

Szalanski, Allen L.

236

The Evolution of the Networks of Observers of Phenomena Arlot, J.-E.  

E-print Network

Jupiter in one day and an half and is eclipsed each time!). It was then easier to date each eclipse were the eclipses of the satellites of Jupiter by the planet Jupiter. As soon as Galileo observed. 1 ­ The principle of the phenomena of the Galilean satellites of Jupiter 35 #12;From Galileo until

Paris-Sud XI, Université de

237

Detonations in Hydrocarbon Fuel Blends J.M. Austin and J.E. Shepherd  

E-print Network

) by fuel-rich combustion or by thermal cracking. Fuel-rich catalytic combustion uses the presence temperatures and relatively little soot. Catalytic combustion of JP-10 was studied by Brabbs and Merritt [1

Low, Steven H.

238

A Recon gurable Testbed Environment for Spacecraft Autonomy Je rey Biesiadecki Abhinandan Jain  

E-print Network

USA Abstract A key goal of NASA's New Millennium Program NMP is the development of technology Testbed Environment ATBE for the NMP Deep Space I comet asteroid rendezvous mission. This simulation user interfaces as well. 1 Introduction A key goal of the New Millennium Program NMP is the development

239

Draft Genome Sequence of Weissella koreensis KCTC 3621T Je Hee Lee,a  

E-print Network

of this species have been repeatedly isolated from kimchi (a traditional Korean fermented food) and are known repeatedly isolated from kimchi (a tradi- tional Korean fermented food), which is composed of various kinds of vegetables (2, 3, 9). Kimchi is well known for human health-promoting properties such as anticarcinogenic

Bae, Jin-Woo

240

Effect of meliaceous seed extracts on growth and survival of Spodoptera frugiperda (J.E. Smith)  

Microsoft Academic Search

Hexane and ethanol extracts of seeds from 10 plant species (including neem—Azadirachta indica A. Juss.) of the family Meliaceae were incorporated into artificial diet at various doses and fed to fall armyworm [Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)] larvae in nochoice tests. All produced significant mortality, reduced larval growth rate, increased time to pupation, or all three, at some

K. L. Mikolajczak; B. W. Zilkowski; R. J. Bartelt

1989-01-01

241

Quand je serai enseignante pour de vrai... : d'un agir imagin vers un agir assum.  

E-print Network

, Diltec, Université Paris 3-Sorbonne Nouvelle Lucile Cadet, Theodile, IUFM Nord ­ Pas-de-Calais Notre France constitue pour l'enseignant encore novice un premier pas dans la construction d'une identité en formation, qui n'ont bien souvent pas encore rencontré un public-classe en contexte institutionnel

Boyer, Edmond

242

On Tomorrow: Gregory J.E. Rawlins, Associate Professor of Computer Science, Indiana University.  

ERIC Educational Resources Information Center

This interview with Gregory Rawlins, a computer science professor and author, discusses future possibilities in computing and the control of technology. Highlights include issues concerned with electronic publishing; machine intelligence; power and control; and the personalization of information on the Internet. (LRW)

TECHNOS, 1999

1999-01-01

243

Inducing Explanatory Process Models from Biological Time Series Pat Langley, Je Shrager, Nima Asgharbeygi, Stephen Bay  

E-print Network

. The Regulation of Photosynthesis Photosynthesis is a complex combination of reactions that are catalyzed of higher plants. These include `light' reactions, which operate only in the light and use absorbed energy models from such input, and we demonstrate its use on the photosynthesis domain. In closing, we consider

Langley, Pat

244

FPGA Clock Network Architecture: Flexibility vs. Area and Julien Lamoureux and Steven J.E. Wilton  

E-print Network

FPGA Clock Network Architecture: Flexibility vs. Area and Power Julien Lamoureux and Steven J.C., Canada {julienl | stevew} @ece.ubc.ca ABSTRACT This paper examines the tradeoffs between flexibility and global clock domains and varying amounts of flexibility at various levels of the clock network. Using

Wilton, Steve

245

JeDEM 3(1): 33-41, 2011 ISSN 2075-9517  

E-print Network

in the planet's 25,000 peer-reviewed research journals across all scholarly and scientific fields. Without planet's c. 25,000 peer-reviewed journals (and conference proceedings), across all scholarly contributions), and traditional on the subject of peer review and peer-reviewed publication (Harnad 1998). 1

Carr, Leslie

246

Dynamic Pricing by Software Agents Je rey O. Kephart and James E. Hanson  

E-print Network

, processing power, or database access rights, as well as more re ned information products and services and the Internet will merge and evolve together into an information economy bustling with billions of economically motivated software agents that exchange informa- tion goods and services with humans and other agents

Greenwald, Amy

247

zaterdag 4 augustus 2007 -18u09:54 Je bent hier: home Kanaal Vrouw Nieuws  

E-print Network

Sheffield University. Volgens de onderzoekers ligt dit aan testosteron. Minder vruchtbaar In de baarmoeder eigenschappen. Dit kan het besluit om te trouwen beïnvloeden. Testosteron vermindert ook de Tweelingzussen met

Lummaa, Virpi

248

UNL COLLEGE OF ENGINEERING 2011 EMPLOYEE AWARDS J.E. Lagerstrom Outstanding Department  

E-print Network

) Biological Systems Engineering Edgerton Innovation Award Research award open to the rank of assistant to the College of Engineering Jinsong Huang, Assistant Professor, Mechanical Engineering Lim Nguyen Carl Nelson, Associate Professor, Mechanical Engineering Kevin Van Cott, Associate Professor

Farritor, Shane

249

A.M. Young C. Torres J.E. Mack C.W. Cunningham  

E-print Network

. For example, Reeb and Avise (1990) suggested that lowering of the sea level and concurrent changes in climate around the Florida peninsula, and which showed little or no morphological variation (e.g. the American southern Florida. These disjunct taxa are so numerous that they have been designated the Carolinian

Young, Alan M.

250

HPV Risk Classification Using Kernel Based Learning Je-Gun Joung 0 1,2  

E-print Network

National Unive rsity ¿ä ¾à ���¯µ�Á¾¹���·¯½º(human papillomavirus: HPV)´� °¨¿°µ�¾ú�» ¶§ °¢Á¾ ¾�¼º Á¾¾ç�» �½ »ö¿¡ ���� �¿³� ±â¹Ý�� �н� ¹æ¹ý�� ¿ì¼ö�� ¼º´��» º¸��´� °Í�¸·� ³ª�¸³µ´�. 1. ¼- ·Ð ���¯µ�Á¾¹���·¯½º(human papillomavirus: HPV)´� ¾à 8kb �� �¯»ó�� ��Á�³ª¼± DNA ¹���·¯½º·�¼- ¿©¼º�� ��±�°æº�¾Ï�» �¯¹��Ï¸ç ¿©·¯ °¡Áö ¾�¼º Á

251

Cryptography in NaCl Daniel J. Bernstein  

E-print Network

Cryptography in NaCl Daniel J. Bernstein Department of Computer Science (MC 152) The University against espionage and sabotage. Programmers can use lower-level functions but are encouraged to use crypto is expressed as a step-by-step procedure for Alice to encrypt and authenticate a packet; NaCl might compute

Bernstein, Daniel

252

Branching ratios for the beta decay of Na-21  

E-print Network

We have measured the beta-decay branching ratio for the transition from Na-21 to the first excited state of Ne-21. A recently published test of the standard model, which was based on a measurement of the beta-nu correlation in the decay of Na-21...

Iacob, V. E.; Hardy, John C.; Gagliardi, Carl A.; Goodwin, J.; Nica, N.; Park, H. I.; Tabacaru, G.; Trache, L.; Tribble, Robert E.; Zhai, Y.; Towner, I. S.

2006-01-01

253

Thyroid Na /I Symporter MECHANISM, STOICHIOMETRY, AND SPECIFICITY*  

E-print Network

Thyroid Na /I Symporter MECHANISM, STOICHIOMETRY, AND SPECIFICITY* (Received for publication, May College of Medicine, Bronx, New York 10461 The rat thyroid Na /I symporter (NIS) was expressed in Xenopus. It is now firmly established that active accumulation of io- dide (I ) by the thyroid gland epithelium

Eskandari, Sepehr

254

Adaptation of Tobacco Cells to NaCl 1  

PubMed Central

Cell lines of tobacco (Nicotiana tabacum L. var Wisconsin 38) were obtained which are adapted to grow in media with varying concentrations of NaCl, up to 35 grams per liter (599 millimolar). Salt-adapted cells exhibited enhanced abilities to gain both fresh and dry weight in the presence of NaCl compared to cells which were growing in medium without NaCl (unadapted cells). Tolerance of unadapted cells and cells adapted to 10 grams per liter NaCl was influenced by the stage of growth, with the highest degree of tolerance exhibited by cells in the exponential phase. Cell osmotic potential and turgor varied through the growth cycle of unadapted cells and cells at all levels of adaptation, with maximum turgor occurring at approximately the onset of exponential fresh weight accumulation. Adaptation to NaCl led to reduced cell expansion and fresh weight gain, while dry weight gain remained unaffected. This reduction in cell expansion was not due to failure of the cells to maintain turgor since cells adapted to NaCl underwent osmotic adjustment in excess of the change in water potential caused by the addition of NaCl to the medium. Tolerance of the adapted cells, as indicated by fresh or dry weight gain, did not increase proportionately with the increase in turgor. Adaptation of these glycophytic cells to NaCl appears to involve mechanisms which result in an altered relationship between turgor and cell expansion. Images Fig. 3 PMID:16664356

Binzel, Marla L.; Hasegawa, Paul M.; Handa, Avtar K.; Bressan, Ray A.

1985-01-01

255

Hydrothermal Synthesis of (K,Na)NbO3 Particles  

NASA Astrophysics Data System (ADS)

(K,Na)NbO3 (KNN) particles were successfully prepared by hydrothermal synthesis. The results showed that Na+ reacted more readily with Nb to form NaNbO3 than K+. For the purpose of obtaining KNN particles with K/Na=1, a mixed alkaline solution with K+/Na+ ratios ranging from 3.5/1 to 4/1 was required as a starting solution. The morphology and size of KNN particles synthesized strongly depended on K/Na ratio in the KNN particles. The KNN particles synthesized from the starting alkaline solution with K+/Na+=3.5/1 were the smallest with a pelletlike morphology affected by NaNbO3- and KNbO3-based particles. Surfactants such as sodium dodecylbenzenesulfonate (SDBS) and sodium hexametaphosphate (SH) were used to synthesize well dispersed and small KNN particles. Platelike KNN particles with 100 nm thickness and 1.5 µm width were obtained in this study.

Zhang, Fan; Han, Lu; Bai, Shan; Sun, Tiedong; Karaki, Tomoaki; Adachi, Masatoshi

2008-09-01

256

UTICAJ N ? -NITRO-L-ARGININ METIL ESTRA NA METABOLIZAM ARGININA I POLIAMINA U MO?DANOM TKIVU PACOVA U TOKU IZLAGANJA MIKROTALASNOM ZRA?ENJU  

Microsoft Academic Search

Izlaganje mikrotalasnom zra?enju (MW), koje se koristi za mobilnu telefoniju, satelitsku komunikaciju, radio emitere, radare, mikrotalasne toplotne ure?aje i medicinsku dijatermiju dovodi do pojave poreme?aja u razli?itim organskim sistemima. Dokazano je da MW mobilnih telefona dovodi do pove?anja nivoa oksidativnog stresa i apoptoze neurona, narušavanja integriteta krvno-mo?dane barijere i poreme?aja dugotrajne memorije i sposobnosti orijentacije. Iz L-arginina se pod dejstvom

Dejan Petkovi?; Vera Markovi?; Jovica Jovanovi?; Maja Jocic

257

Dynamic polarizabilities and Van der Waals coefficients for alkali atoms Li, Na and alkali dimer molecules Li2, Na2 and NaLi  

NASA Astrophysics Data System (ADS)

The present paper gives an account of investigations of the polarizability of the alkali atoms Li, Na, diatomics homonuclear and heteronuclear Li2, Na2 and NaLi at SCF (Self Consistent Field) level of approximation and at correlated level, using a time Time-Dependent Gauge Invariant method (TDGI). Our static polarizability values agree with the best experimental and theoretical determinations. The Van der Waals C6 coefficients for the atom-atom, atom-dimer and dimer-dimer interactions have been evaluated. Les polarisabilités des atomes alcalins Li, Na, et des molécules diatomiques homonucléaires et hétéronucléaire Li2, Na2 et NaLi, ont été calculées au niveau SCF (Self Consistent Field) et au niveau corrélé à partir d'une méthode invariante de jauge dépendante du temps(TDGI). Nos valeurs des polarisabilités statiques sont en accord avec les meilleurs déterminations expérimentales et théoriques. Les coefficients C6 de Van de Waals pour les interactions atome-atome, atome-dimère et dimère-dimère ont également été évalués.

Mérawa, M.; Dargelos, A.

1998-07-01

258

The regio- and stereo-selective reduction of steroidal 4-en-3-ones using Na?S?O?/NaHCO? and CuCl/NaBH?.  

PubMed

This paper describes the regio- and stereoselective reduction of ??-3-keto moiety in certain steroids using Na?S?O?/NaHCO? and CuCl/NaBH?, respectively. Using either one of the two reduction agents in the reaction, the 17-substituents in the D ring were observed to have clearly influenced the stereoselective reduction of 4-ene in the A ring by the so-called conformational transmission effect. Na?S?O?/NaHCO? regioselectively reduced CC at 4-position of 17-substituted-androst-4-en-3-one derivatives to 5?-H-3-one as the main isomer. And as an extended application, Epiandrosterone (11) was further synthesized from androst-4-en-3,17-dione (AD) via four steps. The total yield from this was about 45%. In the presence of CuCl/NaBH?, ??-3-keto conjugated reduction of 17-spirocyclic ethylene ketal protected androst-4-en-3-one derivatives mainly produced 3?-hydroxy-5?-H isomers, at a yield around 81%. Considering the scaffold configuration of 3?-hydroxy-5?-H moiety coincided with that of bile acid analogs, this selective reduction could also be used as an alternative method for the synthetic study of bile acids using AD and its derivatives, which are from the microorganism degradation of natural sterols, as the potential materials. Meanwhile, configurations of the reductive compounds 5b, 6b, 9, 10 and 17e were identified by X-ray diffraction. PMID:24128809

Wang, Chunli; Chen, Xiaoyu; Huang, Yaoqing; Yang, Jesse; Chen, Ying

2013-12-20

259

[Catalytic properties of solubilized brain Na+,K+-ATPase].  

PubMed

The results are presented of a kinetic study of solubilized Na+, K+-ATPase obtained by 0.2+ digitonin from the NaI treated microsome fraction of the bull brain. It is shown that in main kinetic parameters (KmATP, V, pH-optimum, optimal [Na+]/[K+] ratio, etc.) the solubilized Na+, K+-ATPase does not differ essentially from membrane-bound enzyme. These results evidence for the absence of changes in principle for the kinetic behaviour of Na+,K+-ATPase when solubilized with digitonin. Simultaneously there are certain differences for a temperature dependence (the bend position in the Arrhenius anamorphosis) of the solubilized enzyme and its sensitivity to the action of strophanthin K. PMID:219575

Kravtsov, A V

1978-01-01

260

Dissociation of Methane Hydrate in Aqueous NaCl Solutions.  

PubMed

Molecular dynamics simulations of the dissociation of methane hydrate in aqueous NaCl solutions are performed. It is shown that the dissociation of the hydrate is accelerated by the formation of methane bubbles both in NaCl solutions and in pure water. We find two significant effects on the kinetics of the hydrate dissociation by NaCl. One is slowing down in an early stage before bubble formation, and another is swift bubble formation that enhances the dissociation. These effects arise from the low solubility of methane in NaCl solution, which gives rise to a nonuniform spatial distribution of solvated methane in the aqueous phase. We also demonstrate that bubbles form near the hydrate interface in dense NaCl solutions and that the hydrate dissociation proceeds inhomogeneously due to the bubbles. PMID:25237735

Yagasaki, Takuma; Matsumoto, Masakazu; Andoh, Yoshimichi; Okazaki, Susumu; Tanaka, Hideki

2014-10-01

261

The Na-K-ATPase and Calcium-Signaling Microdomains  

NSDL National Science Digital Library

The Na-K-ATPase is an energy-transducing ion pump that converts the free energy of ATP into transmembrane ion gradients. It also serves as a functional receptor for cardiotonic steroids such as ouabain and digoxin. Binding of ouabain to the Na-K-ATPase can activate calcium signaling in a cell-specific manner. The exquisite calcium modulation via the Na-K-ATPase is achieved by the ability of the pump to integrate signals from numerous protein and non-protein molecules, including ion transporters, channels, protein kinases/phosphatases, as well as cellular Na+. This review focuses on the unique properties of the Na-K-ATPase and its role in the formation of different calcium-signaling microdomains.

Jiang Tian (University of Toledo Health Science Campus Physiology and Pharmacology); Zi-jian Xie (University of Toledo Health Science Campus)

2008-08-01

262

NA60 results on thermal dimuons  

NASA Astrophysics Data System (ADS)

The NA60 experiment at the CERN SPS has measured muon pairs with unprecedented precision in 158 A GeV In-In collisions. A strong excess of pairs above the known sources is observed in the whole mass region 0.2< M<2.6 GeV. The mass spectrum for M<1 GeV is consistent with a dominant contribution from ? + ? -? ?? ? + ? - annihilation. The associated ? spectral function shows a strong broadening, but essentially no shift in mass. For M>1 GeV, the excess is found to be prompt, not due to enhanced charm production, with pronounced differences to Drell-Yan pairs. The slope parameter T eff associated with the transverse momentum spectra rises with mass up to the ?, followed by a sudden decline above. The rise for M<1 GeV is consistent with radial flow of a hadronic emission source. The seeming absence of significant flow for M>1 GeV and its relation to parton-hadron duality is discussed in detail, suggesting a dominantly partonic emission source in this region. A comparison of the data to the present status of theoretical modeling is also contained. The accumulated empirical evidence, including also a Planck-like shape of the mass spectra at low p T and the lack of polarization, is consistent with a global interpretation of the excess dimuons as thermal radiation. We conclude with first results on ? in-medium effects.

Arnaldi, R.; Banicz, K.; Borer, K.; Castor, J.; Chaurand, B.; Chen, W.; Cicalò, C.; Colla, A.; Cortese, P.; Damjanovic, S.; David, A.; de Falco, A.; Devaux, A.; Ducroux, L.; En'yo, H.; Fargeix, J.; Ferretti, A.; Floris, M.; Förster, A.; Force, P.; Guettet, N.; Guichard, A.; Gulkanian, H.; Heuser, J. M.; Keil, M.; Kluberg, L.; Li, Z.; Lourenço, C.; Lozano, J.; Manso, F.; Martins, P.; Masoni, A.; Neves, A.; Ohnishi, H.; Oppedisano, C.; Parracho, P.; Pillot, P.; Poghosyan, T.; Puddu, G.; Radermacher, E.; Ramalhete, P.; Rosinsky, P.; Scomparin, E.; Seixas, J.; Serci, S.; Shahoyan, R.; Sonderegger, P.; Specht, H. J.; Tieulent, R.; Usai, G.; Veenhof, R.; Wöhri, H. K.

2009-06-01

263

Electrochemical Migration Characteristics of Eutectic Sn-Pb Solder Alloy in NaCl and Na2SO4 Solutions  

NASA Astrophysics Data System (ADS)

Anodic dissolution and the electrochemical migration characteristics of eutectic Sn-Pb solder alloy in deaerated 0.001% NaCl and Na2SO4 solutions were investigated using anodic polarization and water drop tests. Anodic polarization results revealed that a Pb-rich phase was preferentially ionized in deaerated 0.001% NaCl solution and an Sn-rich phase was predominantly ionized in deaerated 0.001% Na2SO4 solution, which coincides well with the composition of the dendrites formed during water drop tests. X-ray diffraction and photoelectron spectroscopy results showed that the surface oxide film formed on pure Sn in deaerated 0.001% NaCl solution is more stable than that formed on pure Sn in deaerated 0.001% Na2SO4 solution. The surface oxide film formed on pure Pb in deaerated 0.001% Na2SO4 solution is more stable than that formed on pure Sn in deaerated 0.001% NaCl solution. Therefore, the quality of the surface film of eutectic Sn-Pb solder in a chemical environment seems to be critical not only for corrosion resistance, but also for electrochemical migration resistance.

Jung, Ja-Young; Lee, Shin-Bok; Lee, Ho-Young; Joo, Young-Chang; Park, Young-Bae

2009-05-01

264

A semiclassical study of laser-induced atomic fluorescence from Na2, K2 and NaK  

NASA Technical Reports Server (NTRS)

A semiclassical treatment of laser-induced atomic fluorescence for the alkali-dimer systems Na2, K2 and NaK is presented. The variation of the fluorescence intensity with the frequency of the exciting laser photon is studied and a comparison of theoretical results with a set of experimental data is presented.

Yuan, J.-M.; Bhattacharyya, D. K.; George, T. F.

1982-01-01

265

Motiv identitete v romanih Fran?iška in Prijateljstvo pisatelja Fulvia Tomizze  

E-print Network

Motiv identitete, predvsem obmejne identitete, ima v romanih pisatelja Fulvia Tomizze pomembno mesto, saj mu kot pisatelju meje, kot se je sam imenoval, predstavlja rde?o nit v življenju in delu na literarnem podro?ju. ...

Starc, Maja

2013-09-17

266

Making paradigms of verbs and adjectives using a dialect corpus  

E-print Network

Razprava predstavlja izdelavo nare?nega slovarja Tokunoshime (oto?je Amami, Japonska), ki nastaja na podlagi nare?nega korpusa. Analiza korpusnega gradiva in pogovorov z informanti omogo?a oblikovanje glagolskih in ...

Fukushima, Chitsuko

2010-01-01

267

Regulation of Cardiac Ca2+ Channel by Extracellular Na+  

PubMed Central

Hyponatremia is a predictor of poor cardiovascular outcomes during acute myocardial infarction and in the setting of preexisting heart failure [1]. There are no definitive mechanisms as to how hyponatremia suppresses cardiac function. In this report we provide evidence for direct down-regulation of Ca2+ channel current in response to low serum Na+. In voltage-clamped rat ventricular myocytes or HEK 293 cells expressing the L-type Ca2+ channel, a 15 mM drop in extracellular Na+ suppressed the Ca2+ current by ~15%; with maximal suppression of ~30% when Na+ levels were reduced to 100 mM or less. The suppressive effects of low Na+ on ICa, in part, depended on the substituting monovalent species (Li+, Cs+, TEA+), but were independent of phosphorylation state of the channel and possible influx of Ca2+ on Na+/Ca2+ exchanger. Acidification sensitized the Ca2+ channel current to Na+ withdrawal. Collectively our data suggest that Na+ and H+ may interact with regulatory site(s) at the outer recesses of the Ca2+ channel pore thereby directly modulating the electro-diffusion of the permeating divalents (Ca2+, Ba2+). PMID:21349579

Movafagh, Shahrzad; Cleemann, Lars; Morad, Martin

2011-01-01

268

Rigidification of the autolysis loop enhances Na+ binding to thrombin  

PubMed Central

Binding of Na+ to thrombin ensures high activity toward physiological substrates and optimizes the procoagulant and prothrombotic roles of the enzyme in vivo. Under physiological conditions of pH and temperature, the binding affinity of Na+ is weak due to large heat capacity and enthalpy changes associated with binding, and the Kd=80 mM ensures only 64% saturation of the site at the concentration of Na+ in the blood (140 mM). Residues controlling Na+ binding and activation have been identified. Yet, attempts to improve the interaction of Na+ with thrombin and possibly increase catalytic activity under physiological conditions have so far been unsuccessful. Here we report how replacement of the flexible autolysis loop of human thrombin with the homologous rigid domain of the murine enzyme results in a drastic (up to 10-fold) increase in Na+ affinity and a significant improvement in the catalytic activity of the enzyme. Rigidification of the autolysis loop abolishes the heat capacity change associated with Na+ binding observed in the wild-type and also increases the stability of thrombin. These findings have general relevance to protein engineering studies of clotting proteases and trypsin-like enzymes. PMID:21536369

Pozzi, Nicola; Chen, Raymond; Chen, Zhiwei; Bah, Alaji; Di Cera, Enrico

2011-01-01

269

Zna?ilnosti krajše proze slovenskih književnic  

E-print Network

pisateljic vse od njenih za?etkov, to je s preloma iz 19. v 20. stol., pa do so- dobnosti. Izbor avtoric in literarnih besedil opozarja na tematske in jezikovno-slogovne novosti ter posebnosti v njihovi literaturi, pa tudi na bele lise pri raziskovanju... tabuizirane teme iz slovenskega družbenega in družinskega življenja. Ta tradicija je ostala živa vse do danes. Tako besedila avtoric iz prve polovice 20. stoletja mo?no odslikavajo podrejeni položaj ženske v patriarhalni družbi. Prav dobro je tudi razvidno...

Borovnik, Silvija

2013-09-17

270

Zemljepisnojezikoskovna ?lenitev kajkavš?ine ter slovensko-kajkavska jezikovna meja  

E-print Network

: 4 Rotacizem, tj. glasovna sprememba psl. *VžV > *VrV, pri kateri gre za poseben tip leni- zacije (šibitve), in sicer za sonantizacijo (tj. spremembo nezvo?nika v zvo?nik) (Hock 1991: 81–82), je sporadi?no izpri?ana na celotnem južnoslovanskem... jezikovnem ozemlju (Cvetko Orešnik 1987–1988). Zelo verjetno je, da gre pri tem prvotno za glasovno spremembo, ki je bila kasneje na vzhodu južne slovanš?ine odstranjena z izravnavami v t. i. premenjujo?ih se okoljih (psl. *ženet? : *doženet? > jsl. *žene...

Šekli, Matej

2013-01-01

271

Two-frequency lidar technique for mesospheric Na temperature measurements  

NASA Technical Reports Server (NTRS)

This paper describes a new two-frequency lidar for measuring Na temperature profiles. The laser oscillator is tuned to the two operating frequencies by observing the Doppler-free structure of the Na D2 fluorescence spectrum in a vapor cell. The lidar technique and initial observations of the temperature profile between 82 and 102 km are described. Absolute temperature accuracies at the Na layer peak of better than + or - 3 K with a vertical resolution of 1 km and an integration period of approximately 5 min were achieved.

She, C. Y.; Latifi, H.; Yu, J. R.; Alvarez, R. J., II; Bills, R. E.

1990-01-01

272

Ion Program of Na61/Shine at the CERN SPS  

E-print Network

The NA61/SHINE experiment at the CERN SPS aims to discover the critical point of strongly interacting matter and study properties of the onset of deconfinement. These goals will be reached by measurements of hadron production properties in nucleus-nucleus, proton-proton and proton-lead interactions as a function of collision energy and size of the colliding nuclei. Furthermore, NA61/SHINE will perform numerous precision measurements needed for neutrino (T2K) and cosmic-ray (Pierre Auger Observatory and KASCADE) experiments. This paper summarizes physics arguments for the NA61/SHINE ion program and presents the status and plans of the experiment.

M. Gazdzicki; for the NA61/SHINE Collaboration

2008-12-23

273

Dianion formation from anion-alkali metal charge exchange reactions: TCNQ^- + Na -> TCNQ^-- + Na^+  

NASA Astrophysics Data System (ADS)

The interaction of an electron with an anion is characterized by a long-range coulomb repulsion and a short range polarizability attraction giving rise to a coulomb barrier. The permanent addition of an extra electron to a negatively charged anion requires tunneling through the barrier or attachment of the electron over the top of this coulomb barrier followed by disposal of the excess energy. Charge-exchange collisions of an anion with an alkali atom utilize the latter channel to produce permanent dianions with cross sections of ˜1 å^2. We have previously examined the reaction TCNQ-F4^- + Xe -> TCNQ-F4^-- + Xe^+ and reported a delayed threshold and quantum phase interference effects in the charge exchange cross section.ootnotetextS. Yu. Ovchinnikov, et al. Phys. Rev. A, 73, 64704(2006) Employing sodium as the collision partner, the cross section is seen to increase with decreasing energy with a threshold below 180 eV (com). A new apparatus has been constructed to allow measurements down to energies below the expected threshold (˜41 eV, laboratory energy based upon a 1 eV second electron affinity). This method has been used to study the reaction TCNQ^- + NA -> TCNQ^-- + Na^+ and will provide one of the first measurements of second electron affinities for molecular anions.

Smith, Byron; Compton, Robert; Ovchinnikov, Serge; Holm, Anne; Nielsen, Steen

2011-10-01

274

Phyla- and Subtype-Selectivity of CgNa, a Na+ Channel Toxin from the Venom of the Giant Caribbean Sea Anemone Condylactis Gigantea  

PubMed Central

Because of their prominent role in electro-excitability, voltage-gated sodium (NaV) channels have become the foremost important target of animal toxins. These toxins have developed the ability to discriminate between closely related NaV subtypes, making them powerful tools to study NaV channel function and structure. CgNa is a 47-amino acid residue type I toxin isolated from the venom of the Giant Caribbean Sea Anemone Condylactis gigantea. Previous studies showed that this toxin slows the fast inactivation of tetrodotoxin-sensitive NaV currents in rat dorsal root ganglion neurons. To illuminate the underlying NaV subtype-selectivity pattern, we have assayed the effects of CgNa on a broad range of mammalian isoforms (NaV1.2–NaV1.8) expressed in Xenopus oocytes. This study demonstrates that CgNa selectively slows the fast inactivation of rNaV1.3/?1, mNaV1.6/?1 and, to a lesser extent, hNaV1.5/?1, while the other mammalian isoforms remain unaffected. Importantly, CgNa was also examined on the insect sodium channel DmNaV1/tipE, revealing a clear phyla-selectivity in the efficacious actions of the toxin. CgNa strongly inhibits the inactivation of the insect NaV channel, resulting in a dramatic increase in peak current amplitude and complete removal of fast and steady-state inactivation. Together with the previously determined solution structure, the subtype-selective effects revealed in this study make of CgNa an interesting pharmacological probe to investigate the functional role of specific NaV channel subtypes. Moreover, further structural studies could provide important information on the molecular mechanism of NaV channel inactivation. PMID:21833172

Billen, Bert; Debaveye, Sarah; Beress, Laszlo; Tytgat, Jan

2010-01-01

275

Characterization of Na-X, NaA, and coal fly ash zeolites and their amorphous precursors by IR, MAS NMR and XPS  

Microsoft Academic Search

By fusion with sodium hydroxide followed by a hydrothermal reaction, fly ash and Alenriched fly ash were converted into Na-X and Na-A zeolites, respectively. The authentic Na-X, Na-A and fly ash zeolites as well as their amorphous precursors have been characterized by IR, 29Si and 27Al MAS NMR, XPS\\/AES, TG, and comparative ion-exchange studies of Cs and K with Na

N. Shigemoto; S. Sugiyama; H. Hayashi; K. Miyaura

1995-01-01

276

Igre z mimezisom v poljski in slovenski prozi devetdesetih let  

E-print Network

samo svet (zaradi konkretnih ?lovekovih dejanj), ampak je danes druga?en tudi na?in njegovega prebiranja (prim. Eco 1999), ki je vedno odvisen od ?asa in znanja. S posnemanjem, mimezisom že Aristotel ni mislil na trpno kopiranje sveta, saj naj bi... bilo preslikovanje povezano z igranjem resni?nosti v umetnosti, tj. predstavljanjem (S?ownik terminów literackich: 284–285; prim. Arystoteles 1989). Posnemanje in igranje resni?nosti se izvaja v okvirih predstavljenega sveta, ki ga vsebuje fabula...

Tokarz, Bo?ena

2009-02-01

277

Determinants of substrate and cation transport in the human Na+/dicarboxylate cotransporter NaDC3.  

PubMed

Metabolic intermediates, such as succinate and citrate, regulate important processes ranging from energy metabolism to fatty acid synthesis. Cytosolic concentrations of these metabolites are controlled, in part, by members of the SLC13 gene family. The molecular mechanism underlying Na(+)-coupled di- and tricarboxylate transport by this family is understood poorly. The human Na(+)/dicarboxylate cotransporter NaDC3 (SLC13A3) is found in various tissues, including the kidney, liver, and brain. In addition to citric acid cycle intermediates such as ?-ketoglutarate and succinate, NaDC3 transports other compounds into cells, including N-acetyl aspartate, mercaptosuccinate, and glutathione, in keeping with its dual roles in cell nutrition and detoxification. In this study, we construct a homology structural model of NaDC3 on the basis of the structure of the Vibrio cholerae homolog vcINDY. Our computations are followed by experimental testing of the predicted NaDC3 structure and mode of interaction with various substrates. The results of this study show that the substrate and cation binding domains of NaDC3 are composed of residues in the opposing hairpin loops and unwound portions of adjacent helices. Furthermore, these results provide a possible explanation for the differential substrate specificity among dicarboxylate transporters that underpin their diverse biological roles in metabolism and detoxification. The structural model of NaDC3 provides a framework for understanding substrate selectivity and the Na(+)-coupled anion transport mechanism by the human SLC13 family and other key solute carrier transporters. PMID:24808185

Schlessinger, Avner; Sun, Nina N; Colas, Claire; Pajor, Ana M

2014-06-13

278

Calculation of spectroscopic constants and radiative parameters for the B 1?- X 1?+ electronic transitions of NaK, NaRb, and NaCs molecules  

NASA Astrophysics Data System (ADS)

The vibrational, rotational, and centrifugal spectroscopic constants and the radiative parameters (the Einstein coefficients, oscillator strengths, Frank-Condon factors, r v'v? centroids, and wavenumbers of rotational lines) of electronic-vibrational-rotational transitions in the systems of bands B 1? - X 1?+ of NaK (0 ? v' ? 14, 0 ? v? ? 52, j = 0, 30, 50, 70, 80, and 100), NaRb (0 ? v' ? 12, 0 ? v? ? 51, j = 0, 20, 30, 50, 70, 90, 100, and 120), and NaCs (0 ? v' ? 10, 0 ? v? ? 44, j = 0, 30, 50, 70, 90, 100, and 120) molecules, as well as the radiative lifetimes of excited electronic states, are calculated. Calculations are performed based on semiempirical potential curves constructed in this work. The calculated spectroscopic constants and the radiative lifetimes are compared with experimental values.

Smirnov, A. D.

2014-09-01

279

Na double-edge magneto-optic filter for Na lidar profiling of wind and temperature in the lower atmosphere.  

PubMed

A Na double-edge magneto-optic filter is proposed for incorporation into the receiver of a three-frequency Na Doppler lidar to extend its wind and temperature measurements into the lower atmosphere. Two prototypes based on cold- and hot-cell designs were constructed and tested with laser scanning and quantum mechanics modeling. The hot-cell filter exhibits superior performances over the cold-cell filter containing buffer gas. Lidar simulations, metrics, and error analyses show that simultaneous wind and temperature measurements are feasible in the altitude range of 20-50 km using the hot-cell filter and reasonable Na lidar parameters. PMID:19148254

Huang, Wentao; Chu, Xinzhao; Williams, B P; Harrell, S D; Wiig, Johannes; She, C-Y

2009-01-15

280

O, Na, Ba and Eu abundance patterns in open clusters  

E-print Network

Open clusters are historically regarded as single-aged stellar populations representative of star formation within the Galactic disk. Recent literature has questioned this view, based on discrepant Na abundances relative to the field, and concerns about the longevity of bound clusters contributing to a selection bias: perhaps long-lived open clusters are chemically different to the star formation events that contributed to the Galactic disk. We explore a large sample of high resolution Na, O, Ba & Eu abundances from the literature, homogenized as much as reasonable including accounting for NLTE effects, variations in analysis and choice of spectral lines. Compared to a template globular cluster and representative field stars, we find no significant abundance trends, confirming that the process producing the Na-O anti-correlation in globular clusters is not present in open clusters. Furthermore, previously reported Na-enhancement of open clusters is found to be an artefact of NLTE effects, with the open cl...

MacLean, B T; Lattanzio, J

2014-01-01

281

Na+-K+ Pump Regulation and Skeletal Muscle Contractility  

NSDL National Science Digital Library

The present review is written with the specific purpose of analyzing how regulation of the activity and the capacity of the Na+-K+ pumps influences excitability and contractile performance in skeletal muscle.

MD Torben Clausen (University of Aarhus Department of Physiology)

2003-10-01

282

Na decorated B6 cluster and its hydrogen storage properties  

NASA Astrophysics Data System (ADS)

The structures and hydrogen storage properties of sodium atoms decorated B6 clusters are investigated by the B3LYP method with a 6-311+G (d, p) basis set. For NamB6 (m = 1-3) clusters, Na atoms are always inclined to separate far enough from each other and not cluster together on a B6 cluster surface so that each Na atom has sufficient space to bind hydrogen molecules. The hydrogen storage gravimetric density of a two Na atoms decorated B6 cluster is 17.91 wt% with an adsorption energy per H2 molecule (AAE/H2) of 0.6851 kcal·mol-1. The appropriate AAE/H2 and preferable gravimetric density of the two Na atoms decorated B6 cluster complex indicate that it is feasible for hydrogen storage application in ambient conditions.

Ruan, Wen; Wu, Dong-Lan; Luo, Wen-Lang; Yu, Xiao-Guang; Xie, An-Dong

2014-02-01

283

Regulation of Na v channels in sensory neurons.  

PubMed

Voltage-gated Na(+) channels have an essential role in the biophysical properties of nociceptive neurons. Factors that regulate Na(+) channel function are of interest from both pathophysiological and therapeutic perspectives. Increasing evidence indicates that changes in expression or inappropriate modulation of these channels leads to electrical instability of the cell membrane and the inappropriate spontaneous activity that is observed following nerve injury, and that this might contribute to neuropathic pain. The role of Na(v) channels in nociception depends on modulation by factors such as auxiliary beta-subunits, cytoskeletal proteins and the phosphorylation state of neurons. In this review we describe the modulation of Na(v) channels on sensory neurons by auxiliary beta-subunits, protein kinases and cytoskeletal proteins. PMID:16125256

Chahine, Mohamed; Ziane, Rahima; Vijayaragavan, Kausalia; Okamura, Yasushi

2005-10-01

284

Silver, zinc, and copper exchange in a Na-clinoptilolite and resulting effect on antibacterial activity  

Microsoft Academic Search

Ag+–Na+, Zn2+–Na+, and Cu2+–Na+ equilibria for clinoptilolite-rich mineral from Gördes (Western Anatolia) were investigated at 25 °C and 0.1 N total solution normality. While silver exchange was favorable over the whole concentration range, zinc and copper were partially exchanged and preferred only at low concentrations. The standard free energies of exchanges for Ag+–Na+, Zn2+–Na+, and Cu2+–Na+ pairs were found as

Ayben Top; Semra Ülkü

2004-01-01

285

1674 -XIV Encuentro de Latinoamericanistas Espaoles DITADURA MILITAR NA  

E-print Network

1674 - XIV Encuentro de Latinoamericanistas Españoles DITADURA MILITAR NA AM�RICA LATINA E O, significativas decisões sobre o legado autoritário das ditaduras militares na América Latina das décadas de 1970 transição. No entanto, no Brasil, não só não houve responsabilização pelos crimes da ditadura, como há uma

Paris-Sud XI, Université de

286

Measurement of Na +,K +ATPase Activity in Human Skeletal Muscle  

Microsoft Academic Search

There are few published measures of Na+,K+-ATPase activity in human skeletal muscle. This study investigated the suitability of the K+-stimulated 3-O-methylfluorescein phosphatase assay for measurement of Na+,K+-ATPase activity in human skeletal muscle. Factors investigated include enzyme kinetics, sample treatment, and ligand concentration. The addition of ouabain blocked maximal K+-stimulated 3-O-methylfluorescein phosphatase (3-O-MFPase) activity, confirming the specificity of the assay. Activity

Steve F. Fraser; Michael J. McKenna

1998-01-01

287

Vanadate stimulates the pumped movements of Na in skeletal muscle  

Microsoft Academic Search

We have measured the effects of concentrations of vanadate ranging between 0.01 and 10 mM on the22Na efflux of frog sartorius muscles. The addition of vanadate had no effects when concentrations lower than 0.5 mM were used; higher concentrations increased Na efflux. The increase was abolished by the addition of ouabain (10-5M). In muscles pretreated with ouabain vanadate did not

David Erlij

1984-01-01

288

In vivo modification of Na +,K +ATPase activity in Drosophila  

Microsoft Academic Search

We have constructed and characterized transgenic Drosophila lines with modified Na+,K+-ATPase activity. Using a temperature dependent promoter from the hsp70 gene to drive expression of wild-type ? subunit cDNA, we can conditionally rescue bang-sensitive paralysis and ouabain sensitivity of a Drosophila Na+,K+-ATPase ? subunit hypomorphic mutant, 2206. In contrast, a mutant ? subunit (?D369N) leads to increased bang-sensitive paralysis and

Banghua Sun; Peizhang Xu; Weiya Wang; Paul M. Salvaterra

2001-01-01

289

g State of Na2: Vibrational Numbering and Hyperfine Structure  

E-print Network

The 43 g State of Na2: Vibrational Numbering and Hyperfine Structure G. Lazarov,* A. M. Lyyra,*,1 The Na2 43 g state has been studied by continuous-wave (cw) perturbation-facilitated optical of the 43 g (v, N) 4 b3 u (vb, J ) A1 u (vA, J ) 4 X1 g (v , J ) transitions show hyperfine splittings

Huennekens, John

290

Metal Fluoride Complexes of Na,K-ATPase  

PubMed Central

The Na,K-ATPase belongs to the P-type ATPase family of primary active cation pumps. Metal fluorides like magnesium-, beryllium-, and aluminum fluoride act as phosphate analogues and inhibit P-type ATPases by interacting with the phosphorylation site, stabilizing conformations that are analogous to specific phosphoenzyme intermediates. Cardiotonic steroids like ouabain used in the treatment of congestive heart failure and arrhythmias specifically inhibit the Na,K-ATPase, and the detailed structure of the highly conserved binding site has recently been described by the crystal structure of the shark Na,K-ATPase in a state analogous to E2·2K+·Pi with ouabain bound with apparently low affinity (1). In the present work inhibition, and subsequent reactivation by high Na+, after treatment of shark Na,K-ATPase with various metal fluorides are characterized. Half-maximal inhibition of Na,K-ATPase activity by metal fluorides is in the micromolar range. The binding of cardiotonic steroids to the metal fluoride-stabilized enzyme forms was investigated using the fluorescent ouabain derivative 9-anthroyl ouabain and compared with binding to phosphorylated enzyme. The fastest binding was to the Be-fluoride stabilized enzyme suggesting a preformed ouabain binding cavity, in accord with results for Ca-ATPase where Be-fluoride stabilizes the E2-P ground state with an open luminal ion access pathway, which in Na,K-ATPase could be a passage for ouabain. The Be-fluoride stabilized enzyme conformation closely resembles the E2-P ground state according to proteinase K cleavage. Ouabain, but not its aglycone ouabagenin, prevented reactivation of this metal fluoride form by high Na+ demonstrating the pivotal role of the sugar moiety in closing the extracellular cation pathway. PMID:21708939

Cornelius, Flemming; Mahmmoud, Yasser A.; Toyoshima, Chikashi

2011-01-01

291

Stabilization of Na,K–ATPase by ionic interactions  

Microsoft Academic Search

The effect of ions on the thermostability and unfolding of Na,K–ATPase from shark salt gland was studied and compared with that of Na,K–ATPase from pig kidney by using differential scanning calorimetry (DSC) and activity assays. In 1 mM histidine at pH 7, the shark enzyme inactivates rapidly at 20 °C, as does the kidney enzyme at 42 °C (but not at 20 °C). Increasing

Elfrieda Fodor; Natalya U. Fedosova; Csilla Ferencz; Derek Marsh; Tibor Pali; Mikael Esmann

2008-01-01

292

An empirical NaKCa geothermometer for natural waters  

USGS Publications Warehouse

An empirical method of estimating the last temperature of water-rock interaction has been devised. It is based upon molar Na, K and Ca concentrations in natural waters from temperature environments ranging from 4 to 340??C. The data for most geothermal waters cluster near a straight line when plotted as the function log ( Na K) + ?? log [ ??? (Ca) Na] vs reciprocal of absolute temperature, where ?? is either 1 3 or 4 3 depending upon whether the water equilibrated above or below 100??C. For most waters tested, the method gives better results than the Na K methods suggested by other workers. The ratio Na K should not be used to estimate temperature if ??? ( MCa) MNa is greater than 1. The Na K values of such waters generally yield calculated temperatures much higher than the actual temperature at which water interacted with the rock. A comparison of the composition of boiling hot-spring water with that obtained from a nearby well (170??C) in Yellowstone Park shows that continued water-rock reactions may occur during ascent of water even though that ascent is so rapid that little or no heat is lost to the country rock, i.e. the water cools adiabatically. As a result of such continued reaction, waters which dissolve additional Ca as they ascend from the aquifer to the surface will yield estimated aquifer temperatures that are too low. On the other hand, waters initially having enough Ca to deposit calcium carbonate during ascent may yield estimated aquifer temperatures that are too high if aqueous Na and K are prevented from further reaction with country rock owing to armoring by calcite or silica minerals. The Na-K-Ca geothermometer is of particular interest to those prospecting for geothermal energy. The method also may be of use in interpreting compositions of fluid inclusions. ?? 1973.

Fournier, R.O.; Truesdell, A.H.

1973-01-01

293

The thermopower of liquid Na-Sn alloys  

Microsoft Academic Search

The thermopower, Q, of liquid Na-Sn alloys has been measured as a function of temperature and composition. This alloys system shows a non-NFE behaviour in large parts of the composition range. For dilute Na-Sn alloys, it is shown that the measurements are consistent with a very simple phase-shift calculation. For concentrated alloys, a theoretical interpretation is complicated by the existence

J. A. Meijer; W. van der Lugt

1991-01-01

294

Effect of dronedarone on Na + , Ca 2+ and HCN channels  

Microsoft Academic Search

Previous studies showed that amiodarone causes state-dependent inhibition of Na+ channels thereby mediating an atrial-selective drug effect. The aim of the present study was to investigate the impact of\\u000a the new antiarrhythmic compound dronedarone on Na+, Ca2+ and hyperpolarization-activated cyclic nucleotide-gated ion channels. Monophasic action potentials (MAP) and effective refractory\\u000a period (ERP) were studied in arterially perfused left atria and

Roman Bogdan; Heinz Goegelein; Hartmut Ruetten

2011-01-01

295

A stable Na + \\/H + antiporter of thermophilic bacterium PS3  

Microsoft Academic Search

As a first step in the isolation of a stable Na+\\/H+ antiporter, its reaction in sonicated membrane vesicles of thermophilic bacterium PS3 has been characterized. The sonicated vesicles showed quenching of quinacrine fluorescence in either NADH oxidation or ATP hydrolysis. The quenching was reversed by the addition of Na+, Li+, Mn2+, Cd2+, and Co2+, but not of choline+ or Ca2+,

Kimihiko Goto I; Hajime Hirata; Yasuo Kagawa

1980-01-01

296

Reactions of NaCl with Gaseous SO3, SO2, and O2  

NASA Technical Reports Server (NTRS)

Hot corrosion of gas turbine engine components involves deposits of Na2SO4 which are produced by reactions between NaCl and oxides of sulfur. For the present investigation, NaCl single crystals were exposed at 100 to 850 C to gaseous mixtures of SO3, SO2, and O2. The products formed during this exposure depend, primarily, on the temperatures. The four product films were: NaCl-SO3; Na2S2O7; Na2SO4; and NaCl-Na2SO4. The kinetics of the reactions were measured.

Fielder, W. L.; Stearns, C. A.; Kohl, F. J.

1983-01-01

297

Na-caseinate/oil/water systems: emulsion morphology diagrams.  

PubMed

The concentrated (dispersed phase 50-70 wt%) composition space of Na-caseinate, a family of milk proteins, stabilised emulsions was investigated for three different oils: soybean oil, palm olein and tetradecane with pH 6.8 phosphate buffer continuous phase. The variation of emulsion stability and microstructure were explored using static light scattering, diffusion nuclear magnetic resonance, cryo-scanning electron microscopy, rheology and the time varying macroscopic phase separation of the emulsions. For soybean oil and palm olein a rich diversity of emulsion microstructures and stabilities are realised. Five emulsion domains, each having a different microstructure and macroscopic stability have been identified within the composition space probed. For the lowest concentrations of emulsifier bridging flocculation is evident and emulsions are of low stability. Increasing Na-caseinate concentration leads to an increased stability and the existence of distinct individual oil droplets, visualised using cryo-scanning electron microscopy. Further increases in Na-caseinate concentration reduce emulsion stability due to depletion flocculation. Na-caseinate self-assembly is then initiated. At sufficiently high Na-caseinate and/or oil concentrations the continuous phase of the emulsion is a three-dimensional protein network and emulsion stability is again enhanced. At the limits of the emulsion composition space a gel-like paste is formed. The diversity of emulsion microstructure is reduced when tetradecane is the discrete phase. Na-caseinate self-assembly is limited and there is no evidence for formation of a protein network. PMID:22709624

Tan, Hui Lin; McGrath, Kathryn M

2012-09-01

298

Band gap engineering for graphene by using Na+ ions  

NASA Astrophysics Data System (ADS)

Despite the noble electronic properties of graphene, its industrial application has been hindered mainly by the absence of a stable means of producing a band gap at the Dirac point (DP). We report a new route to open a band gap (Eg) at DP in a controlled way by depositing positively charged Na+ ions on single layer graphene formed on 6H-SiC(0001) surface. The doping of low energy Na+ ions is found to deplete the ?* band of graphene above the DP, and simultaneously shift the DP downward away from Fermi energy indicating the opening of Eg. The band gap increases with increasing Na+ coverage with a maximum E g ? 0.70 eV. Our core-level data, C 1s, Na 2p, and Si 2p, consistently suggest that Na+ ions do not intercalate through graphene, but produce a significant charge asymmetry among the carbon atoms of graphene to cause the opening of a band gap. We thus provide a reliable way of producing and tuning the band gap of graphene by using Na+ ions, which may play a vital role in utilizing graphene in future nano-electronic devices.

Sung, S. J.; Lee, P. R.; Kim, J. G.; Ryu, M. T.; Park, H. M.; Chung, J. W.

2014-08-01

299

MoNA and initial measurements with 7He Resonance *  

NASA Astrophysics Data System (ADS)

Of recent interest in the study of exotic nuclei are neutron rich nuclei near and beyond the drip line. Using radioactive beams produced at the National Superconducting Cyclotron Laboratory (NSCL), the Modular Neutron Array (MoNA) detector, and a 4 Tesla sweeper magnet it is possible to study these nuclei. MoNA is a large area, high-efficiency neutron detector consisting of 144 plastic scintillating bars. When used in tandem with the series of detectors and scintillators in the sweeper setup MoNA is capable of detecting neutrons in coincidence with reaction fragments detected at the sweeper. As an initial experiment to test the tandem setup of MoNA and the sweeper a secondary beam of 8Li was produced in the coupled cyclotron facility and the reaction of interest, stripping off a proton to observe the resonance of 7He immediately decaying to 6He+n, was studied with MoNA and the sweeper. The ground state of 7He resonance was observed in the relative velocities of 6He and the neutron. *MoNA collaboration is supported by the NSF. TP, RP acknowledge the support from the NSF Research Experience for Undergraduates program.

Pike, Tina; Pepin, Robert

2004-10-01

300

Clusters of axonal Na+ channels adjacent to remyelinating Schwann cells.  

PubMed

Rat sciatic nerve fibres were demyelinated by injection of lysolecithin and examined at several stages as Schwann cells proliferated, adhered, and initiated remyelination. Immunoperoxidase EM has been used to follow the clustering of Na+ channels that represents an early step in the formation of new nodes of Ranvier. At the peak of demyelination, 1 week post-injection, only isolated sites, suggestive of the original nodes, were labelled. As Schwann cells adhered and extended processes along the axons, regions of axonal Na+ channel immunoreactivity were often found just beyond their leading edges. These channel aggregates were associated only with the axolemma and Na+ channels were not detected on glial membranes. Sites with more than one cluster in close proximity and broadly labelled aggregates between Schwann cells suggested that new nodes of Ranvier formed as neighbouring Na+ channel groups merged. Schwann cells thus seem to play a major role in ion channel distributions in the axolemma. In all of these stages Na+ channel label was found primarily just outside the region of close contact between axon and Schwann cell. This suggests that Schwann cell adherence acts in part to exclude Na+ channels, or that diffusible substances are involved and can act some distance from regions of direct contact. PMID:8835788

Novakovic, S D; Deerinck, T J; Levinson, S R; Shrager, P; Ellisman, M H

1996-06-01

301

Study on the transformation from NaCl-type Na2TiO3 to layered titanate  

NASA Astrophysics Data System (ADS)

NaCl-type crystal structure sodium titanate (Na2TiO3), which exhibits a unit cell parameter of a=4.49 Å, was obtained by high temperature molten salt reaction. An intermediate phase product with layered structure was prepared by leaching the obtained Na2TiO3. We propose that the layered titanate structure is composed of Na2TiO3 and H2O, corresponding to the host-layer and guest-substance, respectively. Furthermore, the crystal structures of layered titanate were optimized by the density functional theory (DFT). This indicates that water molecules are distributed in an orderly manner in the interlayer through the formation of hydrogen-bonded chain. Moreover, the position of the adjacent lamella translates to c/2 along the c-axis after the intercalation of water.

Liu, Y. H.; Zhao, W.; Wang, W. J.; Yang, X.; Chu, J. L.; Xue, T. Y.; Qi, T.; Wu, J. Y.; Wang, C. R.

2012-03-01

302

Copper toxicity in cultured human skeletal muscle cells: the involvement of Na + \\/K + ATPase and the Na + \\/Ca 2+ -exchanger  

Microsoft Academic Search

Copper (Cu2+) intoxication has been shown to induce pathological changes in various tissues. The mechanism underlying Cu2+ toxicity is still unclear. It has been suggested that the Na+\\/K+-ATPase and\\/or a change of the membrane permeability may be involved. In this study we examined the effects of Cu2+ on the Na+ and Ca2+ homeostasis of cultured human skeletal muscle cells using

Ad A. G. M. Benders; Jie Li; Robert A. C. Lock; René J. M. Bindels; Sjoerd E. Wendelaar Bonga; Jacques H. Veerkamp

1994-01-01

303

Na(+) transport, and the E(1)P-E(2)P conformational transition of the Na(+)/K(+)-ATPase.  

PubMed Central

We have used admittance analysis together with the black lipid membrane technique to analyze electrogenic reactions within the Na(+) branch of the reaction cycle of the Na(+)/K(+)-ATPase. ATP release by flash photolysis of caged ATP induced changes in the admittance of the compound membrane system that are associated with partial reactions of the Na(+)/K(+)-ATPase. Frequency spectra and the Na(+) dependence of the capacitive signal are consistent with an electrogenic or electroneutral E(1)P <--> E(2)P conformational transition which is rate limiting for a faster electrogenic Na(+) dissociation reaction. We determine the relaxation rate of the rate-limiting reaction and the equilibrium constants for both reactions at pH 6.2-8.5. The relaxation rate has a maximum value at pH 7.4 (approximately 320 s(-1)), which drops to acidic (approximately 190 s(-1)) and basic (approximately 110 s(-1)) pH. The E(1)P <--> E(2)P equilibrium is approximately at a midpoint position at pH 6.2 (equilibrium constant approximately 0.8) but moves more to the E(1)P side at basic pH 8.5 (equilibrium constant approximately 0.4). The Na(+) affinity at the extracellular binding site decreases from approximately 900 mM at pH 6.2 to approximately 200 mM at pH 8.5. The results suggest that during Na(+) transport the free energy supplied by the hydrolysis of ATP is mainly used for the generation of a low-affinity extracellular Na(+) discharge site. Ionic strength and lyotropic anions both decrease the relaxation rate. However, while ionic strength does not change the position of the conformational equilibrium E(1)P <--> E(2)P, lyotropic anions shift it to E(1)P. PMID:11053130

Babes, A; Fendler, K

2000-01-01

304

Na/Ca Exchange and Na/K-ATPase Function Are Equally Concentrated in Transverse Tubules of Rat Ventricular Myocytes  

PubMed Central

Formamide-induced detubulation of rat ventricular myocytes was used to investigate the functional distribution of the Na/Ca exchanger (NCX) and Na/K-ATPase between the t-tubules and external sarcolemma. Detubulation resulted in a 32% decrease in cell capacitance, whereas cell volume was unchanged. Thus, the surface-to-volume ratio was used to assess the success of detubulation. NCX current (INCX) and Na/K pump current (Ipump) were recorded using whole-cell patch clamp, as Cd-sensitive and K-activated currents, respectively. Both inward and outward INCX density was significantly reduced by ?40% in detubulated cells. INCX density at 0 mV decreased from 0.19 ± 0.03 to 0.10 ± 0.03 pA/pF upon detubulation. Ipump density was also lower in detubulated myocytes over the range of voltages (?50 to +100 mV) and internal [Na] ([Na]i) investigated (7–22 mM). At [Na]i = 10 mM and ?20 mV, Ipump density was reduced by 39% in detubulated myocytes (0.28 ± 0.02 vs. 0.17 ± 0.03 pA/pF), but the apparent Km for [Na]i was unchanged (16.9 ± 0.4 vs. 17.0 ± 0.3 mM). These results indicate that although thet-tubules represent only ?32% of the total sarcolemma, they contribute ?60% to the total INCX and Ipump. Thus, the functional density of NCX and Na/K pump in the t-tubules is 3–3.5-fold higher than in the external sarcolemma. PMID:14581240

Despa, S.; Brette, F.; Orchard, C. H.; Bers, D. M.

2003-01-01

305

Early Age Strength and Workability of Slag Pastes Activated by NaOH and Na 2CO 3  

Microsoft Academic Search

This paper reports the results of an investigation on activation of blast furnace slag with the emphasis on achievement of equivalent one-day strength to Portland cement at normal curing temperatures and reasonable workability. The effects of varying dosages of activators NaOH and Na2CO3 are discussed in terms of strength of mini cylinders and also workability by the mini slump method.

F. Collins; J. G. Sanjayan

1998-01-01

306

NaCl Taste Thresholds in 13 Inbred Mouse Strains  

PubMed Central

Molecular mechanisms of salty taste in mammals are not completely understood. We use genetic approaches to study these mechanisms. Previously, we developed a high-throughput procedure to measure NaCl taste thresholds, which involves conditioning mice to avoid LiCl and then examining avoidance of NaCl solutions presented in 48-h 2-bottle preference tests. Using this procedure, we measured NaCl taste thresholds of mice from 13 genealogically divergent inbred stains: 129P3/J, A/J, BALB/cByJ, C3H/HeJ, C57BL/6ByJ, C57BL/6J, CBA/J, CE/J, DBA/2J, FVB/NJ, NZB/BlNJ, PWK/PhJ, and SJL/J. We found substantial strain variation in NaCl taste thresholds: mice from the A/J and 129P3/J strains had high thresholds (were less sensitive), whereas mice from the BALB/cByJ, C57BL/6J, C57BL/6ByJ, CE/J, DBA/2J, NZB/BINJ, and SJL/J had low thresholds (were more sensitive). NaCl taste thresholds measured in this study did not significantly correlate with NaCl preferences or amiloride sensitivity of chorda tympani nerve responses to NaCl determined in the same strains in other studies. To examine whether strain differences in NaCl taste thresholds could have been affected by variation in learning ability or sensitivity to toxic effects of LiCl, we used the same method to measure citric acid taste thresholds in 4 inbred strains with large differences in NaCl taste thresholds but similar acid sensitivity in preference tests (129P3/J, A/J, C57BL/6J, and DBA/2J). Citric acid taste thresholds were similar in these 4 strains. This suggests that our technique measures taste quality–specific thresholds that are likely to represent differences in peripheral taste responsiveness. The strain differences in NaCl taste sensitivity found in this study provide a basis for genetic analysis of this phenotype. PMID:22293936

Ishiwatari, Yutaka

2012-01-01

307

A kinetic analysis of Na-Li countertransport in human red blood cells  

PubMed Central

We examined the kinetic properties of the interactions between inner and outer cation sites of the Na-Li countertransport system in human red blood cells. Li-stimulated Na efflux [V(Na)] was measured as a function of external Li [(Li)o] and internal Na [(Na)i] contents. At each (Li)o, a Hanes plot of (Na)i/V(Na) vs. (Na)i allowed us to calculate the apparent dissociation constant for internal Na (KiNa) and the maximal rate of Na efflux [Vmax(Na)]. In erythrocytes from 10 different subjects, the Vmax(Na)/KiNa ratios were independent of the external Li concentrations. In other experiments, Na-stimulated Li efflux [V(Li)] was measured as a function of external Na and internal Li contents. In three subjects studied, the Vmax(Li)/KiLi ratios were independent of the external Na concentrations. The data strongly suggest that the countertransport mechanism is consecutive ("ping- pong"). PMID:2420916

1986-01-01

308

Requirement of the Na+/H+ exchanger for tumor growth.  

PubMed

The Na+/H+ exchanger is involved in a variety of cellular processes, including regulation of intracellular pH and possibly the control of cell growth and proliferation. To study the role of the Na+/H+ exchanger in tumor growth, human sodium proton exchanger-deficient (HSPD) mutants were derived from the human bladder carcinoma cell line MGH-U1 (EJ) by the proton suicide selection technique (J. Pouyssegur et al., Proc. Natl. Acad. Sci. USA, 81: 4833-4837, 1984). The HSPD cells were approximately 40% larger and contained approximately 70% more DNA than the parental cells. They were unable to grow in vitro in the absence of bicarbonate at pH less than 7.0, whereas the parental cells grew well at pH greater than or equal to 6.6. This difference in acid sensitivity was abolished in the presence of bicarbonate. In contrast to the parental MGH-U1 cells, the Na+/H+-deficient HSPD cells either failed to grow tumors, or showed severely retarded tumor growth when implanted into immune-deprived mice. This difference in tumor growth was not attributed to differences in cell size and DNA content, because Na+/H+ exchange-competent large cells (HLC), derived during the same proton suicide selection process as the HSPD cells, grew tumors at a rate close to that of the parental cells. Cells derived from the few tumors which grew after implantation of HSPD mutant cells were revertants which had regained Na+/H+ activity. HSPD cells also failed to form spheroids in culture, and the only spheroid formed consisted of revertant cells which had regained both Na+/H+ exchange activity and tumorigenic capacity. These results suggest that the Na+/H+ exchanger is important for tumor growth. PMID:2535690

Rotin, D; Steele-Norwood, D; Grinstein, S; Tannock, I

1989-01-01

309

Increased Na/H antiporter and Na/3HCO3 symporter activities in chronic hyperfiltration. A model of cell hypertrophy  

PubMed Central

The effect of chronic hyperfiltration, a model of cell hypertrophy, on H/HCO3 transporters was examined in the in vivo microperfused rat proximal tubule. Hyperfiltration was induced by uninephrectomy with subsequent increased dietary protein. After 2 wk the hyperfiltration group had a higher glomerular filtration rate (2.21 +/- 0.13 vs. 1.48 +/- 0.12 ml/min), associated with increased kidney weight (1.71 +/- 0.05 vs. 1.23 +/- 0.04 g). HCO3 absorptive rate measured in tubules perfused with an ultrafiltrate-like solution (25 mM HCO3) was higher in the hyperfiltration group (183 +/- 17 vs. 109 +/- 16 pmol/mm per min). The activities of the apical membrane Na/H antiporter and basolateral membrane Na/3HCO3 symporter were assayed using the measurement of cell pH [(2'7')-bis(carboxyethyl)-(5,6)-carboxyfluorescein] in the doubly microperfused tubule in the absence of contact with native fluids. After 2 wk of hyperfiltration Na/H antiporter activity, assayed as the effect of luminal Na removal on cell pH, was increased 114%. Basolateral membrane Na/3HCO3 symporter activity, assayed as the effect of a decrease in peritubular [HCO3] (25 to 5 mM) or in peritubular [Na] (147 to 25 mM) in the absence of luminal and peritubular chloride, was increased 77 and 113%, respectively, in the hyperfiltration group. Steady-state cell pH, measured with physiologic, ultrafiltrate-like luminal and peritubular perfusates, was significantly higher in the hyperfiltration group (7.27 +/- 0.02 vs. 7.14 +/- 0.03). In similar studies, performed 24 h after uninephrectomy and protein feeding, kidney weight was increased 10%, Na/H antiporter activity 39%, and Na/3HCO3 symporter activity 46%. At this time cell pH was not different between the two groups. The results demonstrate that chronic hyperfiltration is associated with parallel increases in Na/H antiporter and Na/3HCO3 symporter activities. If a decrease in cell pH is the signal that triggers these adaptations, it occurs early, and the adaptations can be maintained in the absence of sustained cell acidification. PMID:1849958

1991-01-01

310

[The reaction mechanism of Na, K-ATPase].  

PubMed

To help characterize the Na,K-ATPase active site with enzyme incorporated into phospholipid vesicles, the activities with alternative substrates were compared, 22Na/Na-transport was equivalent with ATP, CTP, carbamylphosphate and acetylphosphate, but slower with CTP, 3-O-methylfluoresceinphosphate (3-O-MFP), nitrophenylphosphate and umbelliferonephosphate. It indicates a slower rate of formation of phosphorylating enzyme complex in conformation position of E1 (E1P) when the second group of substrates is bound with enzyme active center. 22Na/K-transport was half as effective with CTP as with ATP and was far slower with the other substrates. It indicates a more stringent selectivity at the low-affinity site of enzyme in conformation E2 that accelerates the slow step of this transport mode. Although enzyme modification with fluoresceinisothiocyanate blocks the high-affinity site to ATP, the K-phosphatase reaction catalyzed by E2 is retained, even with a substrate, 3-O-MFP, that binds to the adenine pocket. Dimethylsulfoxide inhibits hydrolysis of the nucleotides and of the carboxylic phosphate substrates of the K-phosphatase reaction, but stimulates hydrolysis of the phenolic phosphate substrates (nitrophenylphosphate and umbelliferone phosphate) which normally are hydrolyzed more slowly than the other substrates. On the basis of these data the authors propose the model of Na,K-ATPase active center. PMID:2169914

Robinson, J D; Guerra, M; Davis, R L; Steinberg, M

1990-01-01

311

Links between hydrothermal environments, pyrophosphate, na(+), and early evolution.  

PubMed

The discovery that photosynthetic bacterial membrane-bound inorganic pyrophosphatase (PPase) catalyzed light-induced phosphorylation of orthophosphate (Pi) to pyrophosphate (PPi) and the capability of PPi to drive energy requiring dark reactions supported PPi as a possible early alternative to ATP. Like the proton-pumping ATPase, the corresponding membrane-bound PPase also is a H(+)-pump, and like the Na(+)-pumping ATPase, it can be a Na(+)-pump, both in archaeal and bacterial membranes. We suggest that PPi and Na(+) transport preceded ATP and H(+) transport in association with geochemistry of the Earth at the time of the origin and early evolution of life. Life may have started in connection with early plate tectonic processes coupled to alkaline hydrothermal activity. A hydrothermal environment in which Na(+) is abundant exists in sediment-starved subduction zones, like the Mariana forearc in the W Pacific Ocean. It is considered to mimic the Archean Earth. The forearc pore fluids have a pH up to 12.6, a Na(+)-concentration of 0.7 mol/kg seawater. PPi could have been formed during early subduction of oceanic lithosphere by dehydration of protonated orthophosphates. A key to PPi formation in these geological environments is a low local activity of water. PMID:21461648

Holm, Nils G; Baltscheffsky, Herrick

2011-10-01

312

Na+/H+ Antiport Is Essential for Yersinia pestis Virulence  

PubMed Central

Na+/H+ antiporters are ubiquitous membrane proteins that play a central role in the ion homeostasis of cells. In this study, we examined the possible role of Na+/H+ antiport in Yersinia pestis virulence and found that Y. pestis strains lacking the major Na+/H+ antiporters, NhaA and NhaB, are completely attenuated in an in vivo model of plague. The Y. pestis derivative strain lacking the nhaA and nhaB genes showed markedly decreased survival in blood and blood serum ex vivo. Complementation of either nhaA or nhaB in trans restored the survival of the Y. pestis nhaA nhaB double deletion mutant in blood. The nhaA nhaB double deletion mutant also showed inhibited growth in an artificial serum medium, Opti-MEM, and a rich LB-based medium with Na+ levels and pH values similar to those for blood. Taken together, these data strongly suggest that intact Na+/H+ antiport is indispensable for the survival of Y. pestis in the bloodstreams of infected animals and thus might be regarded as a promising noncanonical drug target for infections caused by Y. pestis and possibly for those caused by other blood-borne bacterial pathogens. PMID:23774602

Minato, Yusuke; Ghosh, Amit; Faulkner, Wyatt J.; Lind, Erin J.; Schesser Bartra, Sara; Plano, Gregory V.; Jarrett, Clayton O.; Hinnebusch, B. Joseph; Winogrodzki, Judith; Dibrov, Pavel

2013-01-01

313

Kinetic comparisons of heart and kidney Na+,K(+)-ATPases.  

PubMed

Most kinetic measurements of the partial reactions of Na(+),K(+)-ATPase have been conducted on enzyme from mammalian kidney. Here we present a kinetic model that is based on the available equilibrium and kinetic parameters of purified kidney enzyme, and allows predictions of its steady-state turnover and pump current in intact cells as a function of ion and ATP concentrations and the membrane voltage. Using this model, we calculated the expected dependence of the pump current on voltage and extracellular Na(+) concentration. The simulations indicate a lower voltage dependence at negative potentials of the kidney enzyme in comparison with heart muscle Na(+),K(+)-ATPase, in agreement with experimental results. The voltage dependence is enhanced at high extracellular Na(+) concentrations. This effect can be explained by a voltage-dependent depopulation of extracellular K(+) ion binding sites on the E2P state and an increase in the proportion of enzyme in the E1P(Na(+))(3) state in the steady state. This causes a decrease in the effective rate constant for occlusion of K(+) by the E2P state and hence a drop in turnover. Around a membrane potential of zero, negligible voltage dependence is observed because the voltage-independent E2(K(+))(2) ? E1 + 2K(+) transition is the major rate-determining step. PMID:22947929

Garcia, Alvaro; Rasmussen, Helge H; Apell, Hans-Jürgen; Clarke, Ronald J

2012-08-22

314

Na[superscript +] binding to meizothrombin desF1  

SciTech Connect

Meizothrombin is the physiologically active intermediate generated by a single cleavage of prothrombin at R320 to separate the A and B chains. Recent evidence has suggested that meizothrombin, like thrombin, is a Na{sup +}-activated enzyme. In this study we present the first X-ray crystal structure of human meizothrombin desF1 solved in the presence of the active site inhibitor PPACK at 2.1 {angstrom} resolution. The structure reveals a Na{sup +} binding site whose architecture is practically identical to that of human thrombin. Stopped-flow measurements of Na{sup +} binding to meizothrombin desF1 document a slow phase of fluorescence change with a k obs decreasing hyperbolically with increasing [Na{sup +}], consistent with the existence of three conformations in equilibrium, E*, E and E:Na{sup +}, as for human thrombin. Evidence that meizothrombin exists in multiple conformations provides valuable new information for studies of the mechanism of prothrombin activation.

Papaconstantinou, M.E.; Gandhi, P.S.; Chen, Z.; Bah, A.; Di Cera, E. (WU-MED)

2009-06-10

315

Ouabain Binding Site in a Functioning Na+/K+ ATPase*  

PubMed Central

The Na+/K+ ATPase is an almost ubiquitous integral membrane protein within the animal kingdom. It is also the selective target for cardiotonic derivatives, widely prescribed inhibitors for patients with heart failure. Functional studies revealed that ouabain-sensitive residues distributed widely throughout the primary sequence of the protein. Recently, structural work has brought some consensus to the functional observations. Here, we use a spectroscopic approach to estimate distances between a fluorescent ouabain and a lanthanide binding tag (LBT), which was introduced at five different positions in the Na+/K+ ATPase sequence. These five normally functional LBT-Na+/K+ ATPase constructs were expressed in the cell membrane of Xenopus laevis oocytes, operating under physiological internal and external ion conditions. The spectroscopic data suggest two mutually exclusive distances between the LBT and the fluorescent ouabain. From the estimated distances and using homology models of the LBT-Na+/K+ ATPase constructs, approximate ouabain positions could be determined. Our results suggest that ouabain binds at two sites along the ion permeation pathway of the Na+/K+ ATPase. The external site (low apparent affinity) occupies the same region as previous structural findings. The high apparent affinity site is, however, slightly deeper toward the intracellular end of the protein. Interestingly, in both cases the lactone ring faces outward. We propose a sequential ouabain binding mechanism that is consistent with all functional and structural studies. PMID:21911500

Sandtner, Walter; Egwolf, Bernhard; Khalili-Araghi, Fatemeh; Sanchez-Rodriguez, Jorge E.; Roux, Benoit; Bezanilla, Francisco; Holmgren, Miguel

2011-01-01

316

Schistosome Na,K-ATPase as a therapeutic target.  

PubMed

Na,K-ATPases are ubiquitous membrane-bound enzymes comprising ? and ? subunits. Here we clone a Na,K-ATPase ? homolog (designated SNaK1?) from the human parasitic platyhelminth, Schistosoma mansoni. The predicted protein is about 33 kDa, and contains a single transmembrane domain and multiple conserved motifs. SNaK1? and its previously cloned ?-subunit counterpart (SNaK1?) are both expressed throughout the schistosome life cycle. In adults, both subunits are detected in the tegumental membrane, likely functioning at the host-parasite interface in Na/K exchange. Both SNaK1 genes can be suppressed by RNAi using target-specific small inhibitory RNAs (siRNAs), and this severely debilitates the parasites both in vitro and in vivo. However, treating schistosomiasis by delivering the siRNAs hydrodynamically to infected mice has no detectable impact on worms. Additionally, treating schistosome-infected mice with the Na,K-ATPase inhibitor, ouabain, is ineffective. Nonetheless, since schistosomes are very susceptible to perturbation in SNaK1 expression, further work to identify other Na,K-ATPase inhibitors as anti-schistosome agents is warranted. PMID:23222953

Da'dara, Akram A; Faghiri, Zahra; Krautz-Peterson, Greice; Bhardwaj, Rita; Skelly, Patrick J

2013-02-01

317

Links Between Hydrothermal Environments, Pyrophosphate, Na+, and Early Evolution  

NASA Astrophysics Data System (ADS)

The discovery that photosynthetic bacterial membrane-bound inorganic pyrophosphatase (PPase) catalyzed light-induced phosphorylation of orthophosphate (Pi) to pyrophosphate (PPi) and the capability of PPi to drive energy requiring dark reactions supported PPi as a possible early alternative to ATP. Like the proton-pumping ATPase, the corresponding membrane-bound PPase also is a H+-pump, and like the Na+-pumping ATPase, it can be a Na+-pump, both in archaeal and bacterial membranes. We suggest that PPi and Na+ transport preceded ATP and H+ transport in association with geochemistry of the Earth at the time of the origin and early evolution of life. Life may have started in connection with early plate tectonic processes coupled to alkaline hydrothermal activity. A hydrothermal environment in which Na+ is abundant exists in sediment-starved subduction zones, like the Mariana forearc in the W Pacific Ocean. It is considered to mimic the Archean Earth. The forearc pore fluids have a pH up to 12.6, a Na+-concentration of 0.7 mol/kg seawater. PPi could have been formed during early subduction of oceanic lithosphere by dehydration of protonated orthophosphates. A key to PPi formation in these geological environments is a low local activity of water.

Holm, Nils G.; Baltscheffsky, Herrick

2011-10-01

318

Biosensor Consists of Na -- Doped Hydroxyapatite Thin Film  

NASA Astrophysics Data System (ADS)

Hydroxyapatite (HAp) surface has an excellent ability of adsorption for functional biomolecules such as protein, DNA and so on. We have investigated the application of the HAp as a suitable material for biosensor. Thin film of the sodium -- doped HAp (Na -- HAp) is prepared in order to decrease the electric resistivity. We have studied variation of the resistance for the Na -- HAp thin films with the adsorption of the functional biomolecules. The sample were prepared by a pulsed laser deposition technique on porous alumina substrate. After the deposition, sample was post -- annealed in O2 / H2O atmosphere in order to crystallize the Na -- HAp. The powder X ray diffraction pattern shows the sample has a pure HAp structure. The gold comb electrodes were evaporated on the sample for the resistance measurement. The sample was set in pure water of 100 ml in a beaker. When Fetal Bovine Serum of 100 ?l was dropped in the beaker, the Na -- HAp shows the drastic change of the AC resistance (at 120 kHz). This result shows that the Na -- HAp will be one of the most effective materials for the biosensor applications.

Nishikawa, Hiroaki; Kawashima, Masami; Kawai, Tomoji

2005-03-01

319

Highly bright multicolor tunable ultrasmall ?-Na(Y,Gd)F?:Ce,Tb,Eu/?-NaYF? core/shell nanocrystals.  

PubMed

Herein, we report highly bright multicolor-emitting ?-Na(Y,Gd)F?:Ce,Tb,Eu/?-NaYF? nanoparticles (NPs) with precise color tunability. First, highly bright sub-20 nm ?-Na(Y,Gd)F?:Ce,Tb,Eu NPs were synthesized via a heating-up method. By controlling the ratio of Eu(3+) to Tb(3+), we generated green, yellow-green, greenish yellow, yellow, orange, reddish orange, and red emissions from the NP solutions via energy transfer of Ce(3+)? Gd(3+)? Tb(3+) (green) and Ce(3+)? Gd(3+)? Tb(3+)? Eu(3+) (red) ions under ultraviolet light illumination (254 nm). Because of Ce(3+) and Gd(3+) sensitization, Tb(3+) ions exhibited strong green emission. The decay time of Tb(3+) emission decreased from 4.0 to 1.4 ms as the Eu(3+) concentration was increased, suggesting that energy was transferred from Tb(3+) to Eu(3+). As a result, Eu(3+) emission peaks were generated and the emission color was transformed from green to red. Monodisperse sub-6 nm ?-Na(Y,Gd)F?:Ce,Tb,Eu NPs were synthesized through a simple reduction of the reaction temperature. Although fine color tunability was retained, their brightness was considerably decreased owing to an increase in the surface-to-volume ratio. The formation of a ?-NaYF? shell on top of the sub-6 nm NP core to produce ?-Na(Y,Gd)F?:Ce,Tb,Eu/?-NaYF? significantly increased the emission intensity, while maintaining the sub-10 nm sizes (8.7-9.5 nm). Quantum yields of the ultrasmall NPs increased from 1.1-6.9% for the core NPs to 6.7-44.4% for the core/shell NPs. Moreover, highly transparent core/shell NP-polydimethylsiloxane (PDMS) composites featuring a variety of colors, excellent color tunability, and high brightness were also prepared. PMID:23945563

Kim, Su Yeon; Woo, Kyoungja; Lim, Kipil; Lee, Kwangyeol; Jang, Ho Seong

2013-10-01

320

The Na(+) transporter, TaHKT1;5-D, limits shoot Na(+) accumulation in bread wheat.  

PubMed

Bread wheat (Triticum aestivum L.) has a major salt tolerance locus, Kna1, responsible for the maintenance of a high cytosolic K(+) /Na(+) ratio in the leaves of salt stressed plants. The Kna1 locus encompasses a large DNA fragment, the distal 14% of chromosome 4DL. Limited recombination has been observed at this locus making it difficult to map genetically and identify the causal gene. Here, we decipher the function of TaHKT1;5-D, a candidate gene underlying the Kna1 locus. Transport studies using the heterologous expression systems Saccharomyces cerevisiae and Xenopus laevis oocytes indicated that TaHKT1;5-D is a Na(+) -selective transporter. Transient expression in Arabidopsis thaliana mesophyll protoplasts and in situ polymerase chain reaction indicated that TaHKT1;5-D is localised on the plasma membrane in the wheat root stele. RNA interference-induced silencing decreased the expression of TaHKT1;5-D in transgenic bread wheat lines which led to an increase in the Na(+) concentration in the leaves. This indicates that TaHKT1;5-D retrieves Na(+) from the xylem vessels in the root and has an important role in restricting the transport of Na(+) from the root to the leaves in bread wheat. Thus, TaHKT1;5-D confers the essential salinity tolerance mechanism in bread wheat associated with the Kna1 locus via shoot Na(+) exclusion and is critical in maintaining a high K(+) /Na(+) ratio in the leaves. These findings show there is potential to increase the salinity tolerance of bread wheat by manipulation of HKT1;5 genes. PMID:25158883

Byrt, Caitlin Siobhan; Xu, Bo; Krishnan, Mahima; Lightfoot, Damien James; Athman, Asmini; Jacobs, Andrew Keith; Watson-Haigh, Nathan S; Plett, Darren; Munns, Rana; Tester, Mark; Gilliham, Matthew

2014-11-01

321

NaOH Recovery from MEROX Tower Waste Stream Using the Electrodialysis Process  

Microsoft Academic Search

Electrodialysis with two different configurations was used for the recovery of NaOH from the waste stream of a MEROX tower. For this purpose, the effects of cell applied voltage and initial Na concentration in the feed solution on NaOH recovery and current efficiency were studied. Results showed that the recovery and current efficiency were improved by increasing the initial Na

Narjes Keramati; Ahmad Moheb; Mohammad Reza Ehsani

2010-01-01

322

Molecular analysis of the Na+ channel blocking actions of the novel  

E-print Network

Molecular analysis of the Na+ channel blocking actions of the novel class I anti-arrhythmic agent of the Na+ channel, and that the drug may produce potent local anaesthetic or anti-arrhythmic action under; RSD 921; class I anti-arrhythmic; Xenopus oocytes; Na+ channel Abbreviations: rBIIA, rat brain IIA Na

Goldin, Alan L.

323

Growth and electronic properties of NaCl on HOPG  

NASA Astrophysics Data System (ADS)

We report the growth of cross-shaped islands of NaCl on highly oriented pyrolytic graphite (HOPG) and discuss the mechanism of formation and growth kinetics within the framework of diffusion limited aggregation (DLA). These structures are investigated using scanning probe microscopy. The shape and structure of these islands can be finely controlled by the deposition conditions. The islands exhibit large atomically flat surfaces which are ideal supports for investigations of the fundamental properties of deposited atoms, molecules or clusters. Bismuth nanostructures were deposited on the NaCl islands and were investigated via scanning tunneling microscopy (STM) and scanning tunneling spectroscopy (STS). The tunneling spectra recorded for particles on NaCl are surprisingly similar to those measured for similar particles on HOPG. We suggest that this is due to a 'dead' layer commonly observed for Bi thin films.

Mahapatra, O.; Kowalczyk, P. J.; Brown, S. A.

2014-02-01

324

HARP and NA61 (SHINE) hadron production experiments  

SciTech Connect

The hadroproduction experiments HARP and NA61 (SHINE) as well as their implications for neutrino physics are discussed. Recent HARP measurements have already been used for precise predictions of neutrino beams in K2K and MiniBooNE/SciBooNE experiments and are also being used to improve the atmospheric neutrino flux predictions and to help in the optimization of neutrino factory and super-beam designs. First preliminary data from NA61 are of significant importance for a precise prediction of a new neutrino beam at J-PARC to be used for the first stage of the T2K experiment. Both HARP and NA61 provide a large amount of input for validation and tuning of hadroproduction models in Monte-Carlo generators.

Popov, Boris A. [LPNHE, 4 place Jussieu, 75005, Paris and DLNP, JINR, Joliot-Curie 6, 141980, Dubna (Russian Federation)

2009-11-25

325

Intrinsic energy resolution of NaI(Tl)1  

NASA Astrophysics Data System (ADS)

The light output for ?10 mm×10 mm and ?75 mm×75 mm NaI(Tl) crystals and energy resolution were measured for ?-ray energies ranging from 16-1333 keV. These measurements enabled the observation of the light yield nonproportionality behavior and allowed the determination of the intrinsic resolution after correcting for the measured resolution for photomultiplier tube (PMT) statistics. The intrinsic resolution was then compared with the nonproportionality component. The latter was calculated using measured electron response, Monte Carlo N Particle code (MCNP4B), and the simplified cascade sequence for NaI(Tl). This comparison allowed the identification of the intrinsic resolution component associated with ?-rays. Consequently, it was shown that the ?-ray component is the most dominant component of the NaI(Tl) intrinsic resolution.

Moszy?ski, M.; Zalipska, J.; Balcerzyk, M.; Kapusta, M.; Mengesha, W.; Valentine, J. D.

2002-05-01

326

Characterization of the Na?/H? antiporter from Yersinia pestis.  

PubMed

Yersinia pestis, the bacterium that historically accounts for the Black Death epidemics, has nowadays gained new attention as a possible biological warfare agent. In this study, its Na?/H? antiporter is investigated for the first time, by a combination of experimental and computational methodologies. We determined the protein's substrate specificity and pH dependence by fluorescence measurements in everted membrane vesicles. Subsequently, we constructed a model of the protein's structure and validated the model using molecular dynamics simulations. Taken together, better understanding of the Yersinia pestis Na?/H? antiporter's structure-function relationship may assist in studies on ion transport, mechanism of action and designing specific blockers of Na?/H? antiporter to help in fighting Yersinia pestis -associated infections. We hope that our model will prove useful both from mechanistic and pharmaceutical perspectives. PMID:22102858

Ganoth, Assaf; Alhadeff, Raphael; Kohen, Dovrat; Arkin, Isaiah T

2011-01-01

327

Superconducting properties and electronic structure of NaBi.  

PubMed

Resistivity, dc magnetization, and heat capacity measurements are reported for superconducting NaBi. T(c), the electronic contribution to the specific heat ?, the ?C(p)/?T(c) ratio, and the Debye temperature are found to be 2.15?K, 3.4?mJ?mol(-1)?K(-2), 0.78, and 140?K respectively. The calculated electron-phonon coupling constant (?(ep) = 0.62) implies that NaBi is a moderately coupled superconductor. The upper critical field and coherence length are found to be 250?Oe and 115?nm, respectively. Electronic structure calculations show NaBi to be a good metal, in agreement with the experiments; the p(x) and p(y) orbitals of Bi dominate the electronic states at the Fermi Energy. PMID:24804822

Kushwaha, S K; Krizan, J W; Xiong, J; Klimczuk, T; Gibson, Q D; Liang, T; Ong, N P; Cava, R J

2014-05-28

328

High-NA HPCS optical fibers for medical diagnosis and treatment  

NASA Astrophysics Data System (ADS)

Hard Plastic Clad Silica (HPCS) optical fibers with pure silica cores have been developed which are robust and have NA(Numerical Aperture)>0.50. Improved clad only HPCS fibers have been produced for both new 'standard' and 'high' NA versions. Based on new cladding formulations, the 'standard' NA fiber has an NA of 0.41, while the new ultrahigh NA fiber has an NA of 0.54. Mechanical strength and preliminary fatigue data are presented along with spectral characterization data. For the first time significant results were obtained for clad only high NA fibers, The fibers are useful for diagnostic and surgical applications. Short to medium length time to failure results, indicate that the static fatigue parameters of the new high numerical aperture (NA) optical fibers are at least as good as those for former standard NA (0.37) HPCS fibers, which is an advance from previous results on the older formulation high NA fibers.

Skutnik, Bolesh J.

2010-02-01

329

Electrogenic 2 Na+/1 H+ exchange in crustaceans.  

PubMed

Hepatopancreatic brush border membrane vesicles of the freshwater prawn, Macrobrachium rosenbergii and the marine lobster, Homarus americanus exhibited 22Na uptake which was Cl-independent, amiloride sensitive, and stimulated by a transmembrane H gradient (Hi greater than Ho). Sodium influx by vesicles of both species were sigmoidal functions of [Na]o, yielding Hill coefficients that were not significantly different (P greater than 0.5) than 2.0. Estimations of half-saturation constants (KNa) were 82.2 mM (prawn) and 280.1 mM (lobster), suggesting a possible adaptation of this transporter to environmental salinity. Trans-stimulation and cis-inhibition experiments involving variable [H] suggested that the exchangers in both species possessed single internal cation binding sites (pK 6.5-6.7) and two external cation binding sites (prawn, pK 4.0 and 5.7; lobster pK 3.5 and 6.1). Similar cis inhibition studies using amiloride as a competitive inhibitor of Na uptake supported the occurrence of dual external sites (prawn, Ki50 and 1520 microM; lobster Ki9 and 340 microM). Electrogenic Na/H exchange by vesicles from both crustaceans was demonstrated using equilibrium shift experiments where a transmembrane potential was used as the only driving force for the transport event. Transport stoichiometries of the antiporters were determined using Static Head analysis where driving forces for cation transfer were balanced using a 10:1 Na gradient, a 100:1 H gradient, and a stoichiometry of 2.0. These electrogenic 2 Na/1 H exchangers appear thermodynamically capable of generating sufficient gastric acidification for organismic digestive activities. PMID:2167376

Ahearn, G A; Franco, P; Clay, L P

1990-07-01

330

A study of the reaction NaO2+ O ? NaO+ O2: Implications for the chemistry of sodium in the upper atmosphere  

NASA Astrophysics Data System (ADS)

Sodium superoxide (NaO2) is believed to be a major sink for meteor-ablated Na in the upper atmosphere. However, the rate constant for the reaction between NaO2 and O is not known, and its determination is the subject of this study. NaO2 was produced by the reaction between Na vapor, an excess of O2, and the carrier gas N2 in the upstream section of a fast flow tube reactor. Excess atomic O was then added, so that a steady state developed in which the sodium was partitioned between its atomic form and a variety of oxides. The steady state fraction of atomic Na, monitored by laser-induced fluorescence, was then observed as a function of [O] and [O2], from which it was deduced that k(NaO2 + O) = (2.2 ± 1.0) × 10-11 cm3 molecule-1 s-1 at 300 K (3? uncertainty). As a prelude to this experiment, the recombination reaction between O2 and Na was studied by the conventional flow tube technique, yielding k(Na + O2 + N2) = (3.14 ± 0.20) ×10-30 (300/T)-(1.52±0.27) cm6 molecule-2 s-1. These experimental results were then incorporated into a one-dimensional model of sodium in the upper atmosphere between 65 and 110 km. It is shown that formation of NaHCO3 rather than NaO2 is the dominant removal process for atomic Na below 90 km. This new model, whose only important assumption is the rate constant for the reaction NaHCO3 + H ? Na + H2CO3, predicts a seasonal variation of the atomic Na layer in excellent agreement with recent lidar observations at 40° and 69°N and also demonstrates that temperature fluctuations produced by gravity waves should induce significant chemical responses in the Na layer below 88 km.

Helmer, Magdalena; Plane, John M. C.

1993-12-01

331

Possible selective adsorption of enantiomers by Na-montmorillonite  

NASA Technical Reports Server (NTRS)

Racemic amino acids including (D,L) alpha-alamine, (D,L) alpha-aminobutyric acid, (D,L) valine, and (D,L) norvaline were incubated with Na-montmorillonite at 100% CEC at three hydrogen ion concentrations, and amino acid adsorption was determined by ion exchange chromatography. Enantiomers were analyzed by gas chromatography. Differences in the quantities of D and L enantiomers in any of the fractions was no larger than a few percent. Although a large difference in the adsorption of the amino acid enantiomers was not observed, the analysis may indicate a small preferential adsorption (0.5-2%) of L-amino acids by Na-montmorillonite.

Friebele, E.; Shimoyama, A.; Ponnamperuma, C.

1981-01-01

332

Tb/Na tobermorite: Thermal behaviour and high temperature products  

NASA Astrophysics Data System (ADS)

By heating a sample of Tb/Na tobermorite we obtained a phase which was identified through its X-ray diffraction (XRD) pattern, as terbium silicate apatite. Subsequently this compound has been directly prepared by solid state reaction and we carried out a structural refinement from XRD data in space group P6 3/ m obtaining cell parameters a=9.39199(4) Å and c=6.84041(5) Å. Terbium silicate apatite heated in melted NaF led to Tb 4O 7 crystals.

Garra, Walter; Marchetti, Fabio; Merlino, Stefano

2009-06-01

333

Supporting Information to "Influence of Salt Purity on Na+  

E-print Network

.5 1.0 1.5 2.0 decrease with time a PA on UP grade 0.6 M NaCl 10-15 min 20-25 min 35-40 min 45-50 min 55-60 min VSFGIntensity(a.u.) increase with time b PA on ACS grade 0.6 M NaCl 10-15 min 20-25 min 35-40 min 45-50 min 55-60 min VSFGIntensity(a.u.) Wavenumber (cm -1 ) 1350 1400 1450 1500 1550 1600 0.0 0

334

Structural Integrity and Microstructure of NA^+ Conducting Ceramics  

NASA Astrophysics Data System (ADS)

Oxides with the general formula of Na1+x Zr2 Six P3-x O12 , known as Nasicon, are fast Na+ ion-conducting materials with important electrochemical applications and many functional properties, often attributed to their unique structural features. Comparative, in situ studies of the limits of structural integrity were performed for selected Nasicon materials, using synchrotron x-ray diffraction and diamond anvil cell technology. We show how different processing conditions produce crystalline structures with specific morphology. We discuss the bulk modulus, the compressibility and the influence of the volume fraction of primary and secondary crystalline phases on the overall Nasicon structural integrity.

Lipinska, Kristina; Kalita, Patricia; Hemmers, Oliver; Sinogeikin, Stanislav; Shebanova, Olga; Yang, Wenge; Mariotto, Gino

2010-03-01

335

Kinetics of 9-aminoacridine block of single Na channels  

PubMed Central

The kinetics of 9-aminoacridine (9-AA) block of single Na channels in neuroblastoma N1E-115 cells were studied using the gigohm seal, patch clamp technique, under the condition in which the Na current inactivation had been eliminated by treatment with N-bromoacetamide (NBA). Following NBA treatment, the current flowing through individual Na channels was manifested by square-wave open events lasting from several to tens of milliseconds. When 9-AA was applied to the cytoplasmic face of Na channels at concentrations ranging from 30 to 100 microM, it caused repetitive rapid transitions (flickering) between open and blocked states within single openings of Na channels, without affecting the amplitude of the single channel current. The histograms for the duration of blocked states and the histograms for the duration of open states could be fitted with a single-exponential function. The mean open time (tau o) became shorter as the drug concentration was increased, while the mean blocked time (tau b) was concentration independent. The association (blocking) rate constant, kappa, calculated from the slope of the curve relating the reciprocal mean open time to 9-AA concentration, showed little voltage dependence, the rate constant being on the order of 1 X 10(7) M-1s-1. The dissociation (unblocking) rate constant, l, calculated from the mean blocked time, was strongly voltage dependent, the mean rate constant being 214 s-1 at 0 mV and becoming larger as the membrane being hyperpolarized. The voltage dependence suggests that a first-order blocking site is located at least 63% of the way through the membrane field from the cytoplasmic surface. The equilibrium dissociation constant for 9-AA to block the Na channel, defined by the relation of l/kappa, was calculated to be 21 microM at 0 mV. Both tau -1o and tau -1b had a Q10 of 1.3, which suggests that binding reaction was diffusion controlled. The burst time in the presence of 9-AA, which is the sum of open times and blocked times, was longer than the lifetime of open channels in the absence of drug. All of the features of 9-AA block of single Na channels are compatible with the sequential model in which 9-AA molecules block open Na channels, and the blocked channels could not close until 9-AA molecules had left the blocking site in the channels. PMID:6090578

1984-01-01

336

NA62 experiment: Measurement of BR(K+ ? ?+?nubar)  

NASA Astrophysics Data System (ADS)

The NA62 experiment aims to measure the very rare kaon decay K+ ? ?+?nubar at the CERN SPS. The expected branching ratio has been recently estimated within the Standard Model to be (8.22 ± 0.84) × 10-11. From the experimental side, 7 events has been found by E787/949 experiments at BNL providing a measurement of the branching ratio of (1.73+1.15-1.05) × 10-10. The aim of NA62 is to collect ~100 events in two years of data taking with a 10% background. A description of the setup and the R&D program is presented.

Cortina Gil, Eduardo

2009-06-01

337

Complex microwave conductivity of Na-DNA powders  

E-print Network

We report the complex microwave conductivity, $\\sigma=\\sigma_1-i\\sigma_2$, of Na-DNA powders, which was measured from 80 K to 300 K by using a microwave cavity perturbation technique. We found that the magnitude of $\\sigma_1$ near room temperature was much larger than the contribution of the surrounding water molecules, and that the decrease of $\\sigma_1$ with decreasing temperature was sufficiently stronger than that of the conduction of counterions. These results clearly suggest that the electrical conduction of Na-DNA is intrinsically semiconductive.

H. Kitano; K. Ota; A. Maeda

2006-08-01

338

Russian Scientific-Educational Project: Kazan-GeoNa-2017  

NASA Astrophysics Data System (ADS)

For the further successful development of educational and scientific - educational activity of the Russian Federation, the Republic Tatarstan, Kazan is offered the national project - the International Center of the Science and the Internet of Technologies. "GeoNa" (Geometry of Nature - "GeoNa" is developed - wisdom, enthusiasm, pride, grandeur), which includes a modern complex of conference halls (up to 4 thousand places), the Center the Internet of Technologies, 3D Planetarium - development of the Moon, PhysicsLand, an active museum of natural sciences, an oceanarium, training a complex «Spheres of Knowledge», botanical and landscape oases.

Gusev, A.; Alibekova, N.

2011-10-01

339

Russian Scientific-Educational Project: Kazan-GeoNa-2017  

NASA Astrophysics Data System (ADS)

For the further successful development of educational and scientific - educational activity of the Russian Federation, the Republic Tatarstan, Kazan is offered the national project - the International Center of the Science and the Internet of Technologies. "GeoNa" (Geometry of Nature - "GeoNa" is developed - wisdom, enthusiasm, pride, grandeur), which includes a modern complex of conference halls (up to 4 thousand places), the Center the Internet of Technologies, 3D Planetarium - development of the Moon, PhysicsLand, an active museum of natural sciences, an oceanarium, training a complex «Spheres of Knowledge», botanical and landscape oases.

Gusev, A.; Alibekova, N.

2012-09-01

340

Molecular dynamics simulation of dipalmitoylphosphatidylserine bilayer with Na+ counterions.  

PubMed Central

We performed a molecular dynamics simulation of dipalmitoylphosphatidylserine (DPPS) bilayer with Na+ counterions. We found that hydrogen bonding between the NH group and the phosphate group leads to a reduction in the area per headgroup when compared to the area in dipalmitoylphosphatidylcholine bilayer. The Na+ ions bind to the oxygen in the carboxyl group of serine, thus giving rise to a dipolar bilayer similar to dipalmitoylphosphatidylethanolamine bilayer. The results of the simulation show that counterions play a crucial role in determining the structural and electrostatic properties of DPPS bilayer. PMID:11916841

Pandit, Sagar A; Berkowitz, Max L

2002-01-01

341

Beta-Gamma-Angular Correlation in Na-20  

E-print Network

Station, Texas 77843 D. P. May and D. M. Tanner Cyclotron Institute, Texas A AM University, College Station, Texas 77843 (Received 19 November 1980) The P-y angular correlation for the decay of "Na has been measured using a symmetric four detector... for the two correlations, we find 2245 1981 The American Physical Society 2246 R. K. YRIBBLK, D. P. MAY, AND D. M. TANNER (5) 6165 C (7) where a'(E,)=E,("Na)+E,("F)=16.6 MeV. By invoking the conserved vector current (CVC) hypothesis, ' the weak...

Tribble, Robert E.; May, D. P.; Tanner, D. M.

1981-01-01

342

Na 2 SO 4 -induced accelerated oxidation (hot corrosion) of nickel  

Microsoft Academic Search

The Na2SO4-induced accelerated oxidation of nickel has been studied at 1000C. It has been found that low oxygen activities in the Na2SO4, which are produced by the formation of NiO, cause the sulfur activity of the Na2SO4 to be increased. Nickel and sulfur from the Na2SO4 combine to form nickel sulfide and the oxide ion activity of the Na2SO4 is

J. A. Goebel; F. S. Pettit

1970-01-01

343

Differential effects of substrates on three transport modes of the Na+/K(+)-ATPase.  

PubMed

With a purified Na+/K(+)-ATPase preparation reconstituted into phospholipid vesicles, Na+/K+, Na+/Na+, and uncoupled Na+ transport were studied using three nucleotides and five substrates of the K(+)-phosphatase reaction that this enzyme also catalyzes. For Na+/K+ exchange, CTP was half as effective as ATP and GTP one-twentieth; of the phosphatase substrates only carbamyl phosphate and 3-O-methylfluorescein phosphate produced significant transport and at merely 1% of the rate with ATP. For Na+/Na+ exchange, comparable rates of transport were produced by ATP, CTP, carbamyl phosphate and acetyl phosphate, although the actual rate of transport with ATP was only 2.4% of that for Na+/K+ exchange; slower rates occurred with GTP (69%), 3-O-methylfluorescein phosphate (51%), and nitrophenyl phosphate (33%). Only umbelliferone phosphate was ineffective. For uncoupled Na+ transport results similar to those for Na+/Na+ exchange were obtained, but the actual rate of transport was still slower, 1.4% of that for Na+/K+ exchange. Thus, not only nucleotides but a variety of phosphatase substrates (which are phosphoric acid mixed anhydrides) can phosphorylate the enzyme at the high-affinity substrate site to form the E1P intermediate of the reaction sequence. Oligomycin inhibited Na+/K+ exchange with ATP by half, but with carbamyl phosphate not at all; with CTP the inhibition was intermediate, one-fourth. By contrast, oligomycin inhibited Na+/Na+ exchange by one-fifth with all three substrates. A quantitative, steady-state kinetic model accounts for the relative magnitudes of Na+/K+ and Na+/Na+ exchanges with ATP, CTP, and carbamyl phosphate as substrates, as well as the extents of inhibition by oligomycin. The model requires that even when Na+ substitutes for K+ a slow step in the reaction sequence is the E2 to E1 conformational transition. PMID:2156564

Guerra, M; Robinson, J D; Steinberg, M

1990-03-30

344

Salicylic acid alleviates NaCl-induced changes in the metabolism of Matricaria chamomilla plants  

Microsoft Academic Search

Influence of 100 mM NaCl and 50 ?M salicylic acid (SA) and their combination on the metabolism of chamomile (Matricaria chamomilla) during 7 days was studied. NaCl reduced growth and selected physiological parameters and SA in combined treatment (NaCl + SA)\\u000a reversed majority of these symptoms. Application of SA reduced NaCl-induced increase of Na+ in the rosettes, but not in the roots. Accumulation of total

Jozef Ková?ik; Bo?ivoj Klejdus; Josef Hedbavny; Martin Ba?kor

2009-01-01

345

In Situ Observation of Dehydration-Induced Phase Transformation from Na2Nb2O6-H2O to NaNbO3  

E-print Network

In Situ Observation of Dehydration-Induced Phase Transformation from Na2Nb2O6-H2O to NaNbO3 Jong at high temperatures. After dehydration at 288 °C, the Na2Nb2O6-H2O becomes significantly destabilized

Wang, Zhong L.

346

Synthesis, photophysical properties, and photocatalytic applications of Bi doped NaTaO3 and Bi doped Na2Ta2O6 nanoparticles  

NASA Astrophysics Data System (ADS)

Phase formation and photophysical properties of bismuth doped sodium tantalum oxide (perovskite, defect pyrochlore) nanoparticles prepared by a hydrothermal method were studied in detail. It was revealed that the synthesis conditions like NaOH concentration and bismuth precursor (NaBiO3·2H2O) markedly affect the crystal structure of sodium tantalum oxide. At low NaOH concentration and high bismuth precursor (NaBiO3·2H2O) content, Bi doped Na2Ta2O6 (defect pyrochlore) phase was predominantly formed, while at higher NaOH concentration, Bi doped NaTaO3 (perovskite) phase was formed. It was observed that the defect pyrochlore (Bi doped Na2Ta2O6) phase was formed and stabilized by the presence of dopant precursor (NaBiO3·2H2O). The chemical analysis of the samples confirmed the doping of Bi3+ cations in both phases. Doping of bismuth enabled visible light absorption up to 500 nm in perovskite and defect pyrochlore type sodium tantalum oxide. Bi doped NaTaO3 samples showed better performance for the photocatalytic degradation of rhodamine B than that of Bi doped Na2Ta2O6, under visible light irritation (?>420 nm). The present results shed light on phase formation of sodium tantalate and these results are useful in understanding properties of NaTaO3 based compounds, synthesized by the hydrothermal method.

Kanhere, Pushkar; Tang, Yuxin; Zheng, Jianwei; Chen, Zhong

2013-12-01

347

ADP+orthophosphate (P(i)) stimulates an Na\\/K pump-mediated coefflux of P(i) and Na in human red blood cell ghosts  

Microsoft Academic Search

The Na\\/K pump in human red blood cells that normally exchanges 3 Nai for 2 Ko is known to continue to transport Na in a ouabain-sensitive and ATP-dependent manner when the medium is made free of both Nao and Ko. Although this Na efflux is called \\

R. Marin

1994-01-01

348

Pancreatic ?-cell Na+ channels control global Ca2+ signaling and oxidative metabolism by inducing Na+ and Ca2+ responses that are propagated into mitochondria.  

PubMed

Communication between the plasma membrane and mitochondria is essential for initiating the Ca(2+) and metabolic signals required for secretion in ? cells. Although voltage-dependent Na(+) channels are abundantly expressed in ? cells and activated by glucose, their role in communicating with mitochondria is unresolved. Here, we combined fluorescent Na(+), Ca(2+), and ATP imaging, electrophysiological analysis with tetrodotoxin (TTX)-dependent block of the Na(+) channel, and molecular manipulation of mitochondrial Ca(2+) transporters to study the communication between Na(+) channels and mitochondria. We show that TTX inhibits glucose-dependent depolarization and blocks cytosolic Na(+) and Ca(2+) responses and their propagation into mitochondria. TTX-sensitive mitochondrial Ca(2+) influx was largely blocked by knockdown of the mitochondrial Ca(2+) uniporter (MCU) expression. Knockdown of the mitochondrial Na(+)/Ca(2+) exchanger (NCLX) and Na(+) dose response analysis demonstrated that NCLX mediates the mitochondrial Na(+) influx and is tuned to sense the TTX-sensitive cytosolic Na(+) responses. Finally, TTX blocked glucose-dependent mitochondrial Ca(2+) rise, mitochondrial metabolic activity, and ATP production. Our results show that communication of the Na(+) channels with mitochondria shape both global Ca(2+) and metabolism signals linked to insulin secretion in ? cells.- Nita, I. I., Hershfinkel, M., Kantor, C., Rutter, G. A., Lewis, E. C., Sekler, I. Pancreatic ?-cell Na(+) channels control global Ca(2+) signaling and oxidative metabolism by inducing Na(+) and Ca(2+) responses that are propagated into mitochondria. PMID:24719357

Nita, Iulia I; Hershfinkel, Michal; Kantor, Chase; Rutter, Guy A; Lewis, Eli C; Sekler, Israel

2014-08-01

349

Ability of multicellular salt glands in Tamarix species to secrete Na+ and K+ selectively.  

PubMed

The present study aimed to determine the mechanism of cation-selective secretion by multicellular salt glands. Using a hydroponic culture system, the secretion and accumulation of Na(+) and K(+) in Tamarix ramosissima and T. laxa under different salt stresses (NaCl, KCl and NaCl+KCl) were studied. Additionally, the effects of salt gland inhibitors (orthovanadate, Ba(2+), ouabain, tetraethylammonium (TEA) and verapamil) on Na(+) and K(+) secretion and accumulation were examined. Treatment with NaCl (at 0-200 mmol L(-1) levels) significantly increased Na(+) secretion, whereas KCl treatment (at 0-200 mmol L(-1) levels) significantly increased K(+) secretion. The ratio of secretion to accumulation of Na(+) was higher than that of K(+). The changes in Na(+) and K(+) secretion differed after adding different ions into the single-salt solutions. Addition of NaCl to the KCl solution (at 100 mmol L(-1) level, respectively) led to a significant decrease in K(+) secretion rate, whereas addition of KCl to the NaCl solution (at 100 mmol L(-1) level, respectively) had little impact on the Na(+) secretion rate. These results indicated that Na+ secretion in Tamarix was highly selective. In addition, Na(+) secretion was significantly inhibited by orthovanadate, ouabain, TEA and verapamil, and K(+) secretion was significantly inhibited by ouabain, TEA and verapamil. The different impacts of orthovanadate on Na(+) and K(+) secretion might be the primary cause for the different Na(+) and K(+) secretion abilities of multicellular salt glands in Tamarix. PMID:21416329

Ma, Haiyan; Tian, Changyan; Feng, Gu; Yuan, Junfeng

2011-03-01

350

Electron transfer and ionization in collisions of He-like ions with Na(3s) and Na(3p)  

NASA Astrophysics Data System (ADS)

Single electron transfer and ionization in collisions of He-like ions (N5+, O6+, Ne8+) and Na has been investigated both experimentally and theoretically at energies around the matching velocity of the valence electron (2 to 10 keV/amu). State selective cross sections and scattering angle distributions were obtained using recoil-ion momentum spectroscopy in combination with a magneto-optically cooled Na atom target. A strong dependence of the relative cross sections on the collision energy is observed. The results are compared with Classical-Trajectory Monte Carlo (CTMC) calculations and show an overall very good agreement.

Blank, I.; Otrantcr, S.; Meinema, C.; Olson, R. E.; Hoekstra, R.

2012-11-01

351

Review: Silvija Borovnik: Književne Študije. O vlogi ženske v slovenski književnosti, o sodobni prozi in o slovenski književnosti v Avstriji  

E-print Network

praznemu, navideznemu življe- nju. Prav tako je postmodernisti?na tudi proza Tamare Doneve, ki tako v kratki prozi kot tudi v romanih preigrava raz- li?ne vzorce iz literarne in neliterarne tradicije. Za kratko prozo Maje B. Jan- ?i? avtorica zapiše, da... ta jezik nudil svobodo. Podobno odlo?itev je sprejela tudi Maja Haderlap v proznem prven- cu Engel des Vergessens, ko se je tudi zaradi svobodnejšega literarnega izraza raje odlo?ila za nemš?ino. O tem bere- mo v za?etnem prispevku tretjega dela...

Lubej, Janja Vollmaier

2012-02-01

352

Stereotipi o reformaciji: primer odpustkov  

E-print Network

svetnikov pravilnem ?aš?enju pa gl. npr. Katekizem z dvema izlagama (Zbrana dela 2); njihov cerkveni koledar ima lepo po redu naštete vse svetnike, enako kot katoliški, gl. npr. Ta slovenski kolendar, 1557 in 1582 (Zbrana dela 3). Tudi za Trubarja je... rymske velike odpuske okuli pošylal; ž njimi je on vsem ludem vse grehe odpustil« (Artikuli, Zbrano dela 3, 41). To dvoje ni res. Od- pustki oz. odpustek ni odpuš?anje grehov, ampak odpuš?anje oz. zmanjšanje ?asne kazni za grehe. Zato tudi ni res, da je...

Bjel?evi?, Aleksander

2008-01-01

353

Problem znanstvenega komuniciranja. Zakaj potrebujemo gibanje Prosti dostop.  

E-print Network

raziskovalca le še zadnji korak k objavi, ki jo nujno potrebuje za ob- stoj v akademskem svetu, kjer prevladuje na?elo objavi ali umri (»publish or perish«). Problem je še posebej pere? med profesorji novinci brez t. i. tenure (assistant profes- sors, ki v... in njihovih katalogov upada sorazmerno s porastom uporabe elektron- skih iskalnikov. Si kdo upa napovedati, da se bo ta trend obrnil? Kateri bralstva željni raziskova- lec pa ho?e ostati neviden elektronskim iskalnikom? Jasno je, da je prihodnost...

Greenberg, Marc L.; Emmett, Ada

2010-09-04

354

Obravnava bolnika z gastroezofagealno refluksno boleznijo v ambulanti zdravnika dru?inske medicine Treatment of the patient with gastro-oesophageal reflux disease in family practice  

Microsoft Academic Search

Izvle?ek: Gastroezofagealna refluksna bolezen je pogosta, potencialno resna te?ava, ki bolnikom mo?no poslabša kakovost ?ivljenja. Zanjo je zna?ilna zgaga in zatekanje ?elod?ne vsebine iz ?elodca v po?iralnik. Pri postavitvi diagnoze je klju?nega pomena natan?en pogovor z bolnikom in prou?itev njegovih te?av. Diagnozo lahko potrdimo z diagnosti?nim testom. Pri ve? kot 50% bolnikov ob endoskopski preiskavi ne najdemo sprememb na sluznici

Zora BOJC

355

Elucidation of the Na+, K+-ATPase digitalis binding site.  

PubMed

Despite controversy over their use and the potential for toxic side effects, cardiac glycosides have remained an important clinical component for the treatment for congestive heart failure (CHF) and supraventricular arrhythmias since the effects of Digitalis purpurea were first described in 1785. While there is a wealth of information available with regard to the effects of these drugs on their pharmacological receptor, the Na(+), K(+)-ATPase, the exact molecular mechanism of digitalis binding and inhibition of the enzyme has remained elusive. In particular, the absence of structural knowledge about Na(+), K(+)-ATPase has thwarted the development of improved therapeutic agents with larger therapeutic indices via rational drug design approaches. Here, we propose a binding mode for digoxin and several analogues to the Na(+), K(+)-ATPase. A 3D-structural model of the extracellular loop regions of the catalytic alpha1-subunit of the digitalis-sensitive sheep Na(+), K(+)-ATPase was constructed from the crystal structure of an E(1)Ca(2+) conformation of the SERCA1a and a consensus orientation for digitalis binding was inferred from the in silico docking of a series of steroid-based cardiotonic compounds. Analyses of species-specific enzyme affinities for ouabain were also used to validate the model and, for the first time, propose a detailed model of the digitalis binding site. PMID:15886034

Keenan, Susan M; DeLisle, Robert K; Welsh, William J; Paula, Stefan; Ball, William J

2005-06-01

356

Optimization of Automated Float Glass Lines Byungsoo Na, Shabbir Ahmed  

E-print Network

Optimization of Automated Float Glass Lines Byungsoo Na, Shabbir Ahmed , George Nemhauser and Joel of Scope and Purpose Flat glass is approximately a $20 billion/year industry worldwide, with almost all flat glass products being manufactured on float glass lines. New technologies are allowing float glass

Ahmed, Shabbir

357

Stainless Steel NaK Circuit Integration and Fill Submission  

NASA Technical Reports Server (NTRS)

The Early Flight Fission Test Facilities (EFF-TF) team has been tasked by the Marshall Space Flight Center Nuclear Systems Office to design, fabricate, and test an actively pumped alkali metal flow circuit. The system, which was originally designed to hold a eutectic mixture of sodium potassium (NaK), was redesigned to hold lithium; but due to a shift in focus, it is once again being prepared for use with NaK. Changes made to the actively pumped, high temperature loop include the replacement of the expansion reservoir, addition of remotely operated valves, and modification of the support table. Basic circuit components include: reactor segment, NaK to gas heat exchanger, electromagnetic (EM) liquid metal pump, load/drain reservoir, expansion reservoir, instrumentation, and a spill reservoir. A 37-pin partial-array core (pin and flow path dimensions are the same as those in a full design) was selected for fabrication and test. This document summarizes the integration and fill of the pumped liquid metal NaK flow circuit.

Garber, Anne E.

2006-01-01

358

New Measurement of ?-? Angular Correlation in ^22Na  

NASA Astrophysics Data System (ADS)

We have measured the ?-? directional correlation in the hindered decay ^22 Na (3^+) arrow ^22Ne (2^+) using the Gammasphere array at LBNL. Our measurement contributes to determining a complete set of weak form factors for the ^22Na ? decay, testing corrections to allowed ? decay to second recoil order. The aim is to resolve a long-standing discrepancy between experiment and allowed-order theory in the electron capture to positron emission ratios. We used a 6 ?Ci source of ^22Na in the center of Gammasphere, a spherical array of 110 Ge detectors. The ?^+ is detected with a 3 mm thick plastic scintillator with 8% solid angle. The ?^+ decay is followed by a 1.275 MeV ? from the ^22Ne 2^+ state. We search for a directional dependence of the emitted ?'s detected in coincidence with a ? of form 1 + A\\cos^2 ?, with ? the angle between the ? and the individual Ge detectors, and A of order 10-3. Variable Ge detector efficiency is normalized by dividing coincident ? counts by single 1.275 MeV ?'s. Fitting the results versus ? - ? angle using gates on the ?^+ energy yields the energy-dependent A, which is sensitive to a linear combination of second-order form factors. Current data and analysis, and implications for ^22Na form factors will be presented.

Vetter, Paul; Bowers, Chris; Freedman, Stuart; Fujikawa, Brian; Reich, Jurgen; Shang, Song; Wasserman, Eric

1997-10-01

359

Free Energy for the Permeation of Na+ Ions and Their  

E-print Network

Free Energy for the Permeation of Na+ and Cl- Ions and Their Ion-Pair through a Zwitterionic-mail: ikhavru@mccammon.ucsd.edu Abstract: Understanding the mechanism of ion permeation across lipid bilayers for the ion permeation in disagreement with predictions from the simple electrostatic models. In this paper we

Lu, Benzhuo

360

High precision measurements of Na-26 beta(-) decay  

E-print Network

High-precision measurements of the half-life and beta-branching ratios for the beta(-) decay of Na-26 to Mg-26 have been measured in beta-counting and gamma-decay experiments, respectively. A 4 pi proportional counter and fast tape transport system...

Grinyer, GF; Svensson, CE; Andreoiu, C.; Andreyev, AN; Austin, RAE; Ball, GC; Chakrawarthy, RS; Finlay, P.; Garrett, PE; Hackman, G.; Hardy, John C.; Hyland, B.; Iacob, VE; Koopmans, KA; Kulp, WD; Leslie, JR; Macdonald, JA; Morton, AC; Ormand, WE; Osborne, CJ; Pearson, CJ; Phillips, AA; Sarazin, F.; Schumaker, MA; Scraggs, HC; Schwarzenberg, J.; Smith, MB; Valiente-Dobon, JJ; Waddington, JC; Wood, JL; Zganjar, EF.

2005-01-01

361

Transport of aromatic molecules in NaY zeolite powders  

SciTech Connect

This paper reports on Xenon-120 NMR that is used to probe macroscopic distributions of aromatic molecules adsorbed in a packed bed of 1-{mu}m NaY zeolite particles. Relative rates of guest transport through the intracrystalline (micro) and intercrystalline (macro) pores play a unique role in the axial distribution of sorbate molecules, such as hexamethylbenzene, in a zeolite powder. Xenon-129 NMR spectra show that a sharp HMB adsorption front advances through a bed of dehydrated NaY crystallites at 523 K. However, at 573 K or in the presence of coadsorbed water, HMB species disperse through the bed without forming a sharp boundary between adsorption zones. When guest transport is controlled by pseudosteady-state diffusion in the macropores, axial penetration of the bed by vapor-phase guest species occurs in a sharp adsorption front. A shrinking-core transport model then quantitatively estimates the intracrystalline diffusivities of HMB in dehydrated and partially hydrated NaY zeolite of 10{sup {minus}11} and 10{sup {minus}13} m{sup 2}/s, respectively, at 523 K. Xenon- 129 NMR proves to be a powerful tool for probing adsorbed guest distribution in zeolites, allowing relative time scales to be established for transport of molecular guests in NaY powders.

Chmelka, B.F.; Gillis, J.V.; Petersen, E.E.; Radke, C.J.

1990-10-01

362

FEORAGAN RUADH NA GAIDHEALTACHD Scottish Squirrel Group Update September 2011  

E-print Network

FEORAGAN RUADH NA GAIDHEALTACHD Scottish Squirrel Group Update September 2011 Strongholds Guidance is in production. Pilot sites are underway, Julia Garritt to update the meeting. Grey squirrel news No further records since Skye in December 2010. Plan to set up network of grey squirrel `panic bags' around

363

Grant Title: WALLACE FOUNDATION Funding Opportunity Number: N/A  

E-print Network

Education: Expanding arts learning opportunities for children and teens in school and beyond; (5) AudienceGrant Title: WALLACE FOUNDATION Funding Opportunity Number: N/A Agency/Department: The Wallace this approach strengthens the effectiveness of their funding. But it also means Wallace rarely funds unsolicited

Farritor, Shane

364

Grant Title: COOPER FOUNDATION Funding Opportunity Number: N/A  

E-print Network

of significant promise. Special funding priorites are given to: education, human services, arts, humanities its capital in support of people through education, human services, the arts, the humanitiesGrant Title: COOPER FOUNDATION Funding Opportunity Number: N/A Agency/Department: Cooper Foundation

Farritor, Shane

365

Benefiting from Polarization: Effects at High-NA Imaging  

E-print Network

157nm F2 Laser Mirror MirrorMirror Illuminator Mask Projection Lens Wafer Polarizer F2 Laser Mirror MirrorMirror Illuminator Mask Projection Lens Wafer Polarizer linearly polarized output beam unpolarizedBenefiting from Polarization: Effects at High-NA Imaging Bruce W. Smith L. Zavyalova, A. Estroff, Y

Zanibbi, Richard

366

National Aeronautics and Space Administration NaNotechNology Roadmap  

E-print Network

National Aeronautics and Space Administration · NaNotechNology Roadmap Technology Area 10 Michael A of fourteen technology area roadmaps, recommending the overall technology investment strategy in these areas, NASA plans updates to its integrated technology roadmap on a regular basis. #12;exeCuTive Summary

Waliser, Duane E.

367

Isolation of22Na for intense positron sources  

NASA Astrophysics Data System (ADS)

A method of removing22Na from Al via distillation is investigated. Distillation is rapid (?10 minutes) and nearly quantitative. When the distilled vapor is directed by a flow of He gas the deposit may be localized fairly well and easily recovered with water.

Griffin, H. C.; Steiger, T. D.; van House, J.; Skalsey, M.; Conti, R.; Rich, A.; Zitzewitz, P. W.

1989-03-01

368

Regulation of Intracellular Cholesterol Distribution by Na/K-ATPase*  

E-print Network

Regulation of Intracellular Cholesterol Distribution by Na/K-ATPase* Received for publication/K-ATPase 1 subunit produces a parallel decrease in both caveolin-1 and cholesterol in light fractions of LLC redistribution of cholesterol from the plasma membrane to intracellular compartments in the knockdown cells

Brand, Paul H.

369

Exploring Neutron-Rich Oxygen Isotopes with MoNA  

E-print Network

The Modular Neutron Array (MoNA) was used in conjunction with a large-gap dipole magnet (Sweeper) to measure neutron-unbound states in oxygen isotopes close to the neutron dripline. While no excited states were observed in 24O, a resonance at 45(2) keV above the neutron separation energy was observed in 23O.

N. Frank; T. Baumann; D. Bazin; J. Brown; P. A. DeYoung; J. E. Finck; A. Gade; J. Hinnefeld; R. Howes; J. -L. Lecouey; B. Luther; W. A. Peters; H. Scheit; A. Schiller; M. Thoennessen

2007-08-20

370

Optical Attenuation in MoNA and LISA Detector Elements  

NASA Astrophysics Data System (ADS)

The MoNA collaboration is a research group of students and faculty from 13 primarily undergraduate institutions, with detectors at the NSCL: MoNA (Modular Neutron Array) and the newly-built LISA (Large multi-Institutional Scintillating Array). These arrays each have 144 plastic scintillating bars. When a neutron collides with a hydrogen nucleus within the plastic, photomultiplier tubes at either end of the bar detect the scintillation photons. Their arrival times are used to determine the position of the event, but as the light travels through the detector it loses intensity exponentially. How dramatic this loss is can be described by a parameter called the attenuation length, with larger attenuation lengths corresponding to lower loss. Recently the MoNA collaboration conducted its LISA commissioning experiment investigating two-neutron decay states of ^25O. As a part of LISA's commissioning, we measured the attenuation lengths of the individual detector bars that make up the LISA array and compared these lengths with those of the older MoNA array. We found that the LISA bars had a larger attenuation length on average with impacts on detector efficiency and effective threshold.

Rice, Logan; Wong, Jonathan

2011-10-01

371

Grant Title: STARR FOUNDATION Funding Opportunity Number: N/A  

E-print Network

Grant Title: STARR FOUNDATION Funding Opportunity Number: N/A Agency/Department: Starr Foundation: The Foundation funds organizations that provide need-based financial aid to students seeking to attend secondary. The Foundation makes significant grants to large cultural institutions, smaller arts organizations, community

Farritor, Shane

372

Synthesis of Na-zeolites from fly ash  

Microsoft Academic Search

The present study focuses on the synthesis of zeolites after fly ash activation, the major objectives being: (a) to study the activation processes; (b) to elucidate the activation conditions for maximum synthesis efficiency; (c) to optimize the conditions for the monomineral synthesis; and (d) to test synthesis efficiencies for different fly ash types. Fly ash was activated by NaOH and

Xavier Querol; Felicià Plana; Andrés Alastuey; Angel López-Soler

1997-01-01

373

A Lepton Universality Test at CERN NA62 Experiment  

E-print Network

The NA62 experiment at CERN collected a large sample of K+ --> enu decays during a dedicated run in 2007, aiming at a precise test of lepton universality by measurement of the helicity suppressed ratio RK = BR(K+ --> enu)/BR(K+ --> munu). A preliminary result of the analysis of a partial data sample of 51089 K+ --> enu candidates is presented.

Evgueni Goudzovski

2010-05-07

374

POWER FROM NA COOLED REACTOR REACTOR DESIGN AND FEASIBILITY PROBLEM  

Microsoft Academic Search

Work done on the study of a Na cooled, graphite moderated nuclear ; reactor for power production is summarized. The preliminary design calculations ; and results are described for a heterogeneous, thermal reactor using rod type ; fuel elements of slightly enriched U. The total heat capacity is 300,000 kw with ; a nominal electric power producing capacity of 75,000

D. R. Bennion; R. L. Hickmott; N. A. Krohn; W. J. McCarthy; R. A. Moore; H. J. Stumpf; L. A. Waldman

1952-01-01

375

Monitorização eletroencefalográfica ambulatorial na epilepsia de difícil controle da infância  

Microsoft Academic Search

RESUMO - O objetivo deste estudo foi analisar através da monitorização eletroencefalográfica ambulatorial contínua e prolongada a distribuição temporal de descargas paroxísticas em sono e em vigília de crianças e adolescentes com epilepsia de difícil controle medicamentoso. Foram selecionadas 21 pacientes na faixa etária de 4 a 17 anos de idade com epilepsia de difícil controle medicamentoso, sendo 52,3 %

Sueli Rizzutti; Mauro Muszkat; Carlos José Reis de Campos

2001-01-01

376

Adolescência e violência: a psicanálise na prática social  

Microsoft Academic Search

Este projeto foi iniciado em 1994, culminando em 97 com a publicação do livro Adolescência e Violência: Conseqüências da Rea- lidade Brasileira (Levisky,1997a). Damos continuidade a esta iniciativa, abordando o binômio ado- lescência e violência a partir da apreensão psicanalítica destes fenôme- nos e seus desdobramentos na prática social. São trabalhos oriundos da experiência clínica de psicanalistas, em sua maioria,

David Léo Levisky

377

Altered erythrocyte Na-K pump in anorectic patients  

SciTech Connect

The status of the erythrocyte sodium pump was evaluated in a group of patients suffering from anorexia nervosa and a group of healthy female control subjects. Anorectic patients showed significantly higher mean values of digoxin-binding sites/cell (ie, the number of Na-K-ATPase units) with respect to control subjects while no differences were found in the specific /sup 86/Rb uptake (which reflects the Na-K-ATPase activity) between the two groups. A significant correlation was found between relative weight and the number of Na-K-ATPase pump units (r = -0.66; P less than 0.0001). Anorectic patients showed lower serum T3 concentrations (71.3 +/- 53 ng/dL) with respect to control subjects (100.8 +/- 4.7 ng/dL; P less than 0.0005) and a significant negative correlation between T3 levels and the number of pump units (r = -0.52; P less than 0.003) was found. This study therefore shows that the erythrocyte Na-K pump may be altered in several anorectic patients. The authors suggest that this feature could be interrelated with the degree of underweight and/or malnutrition.

Pasquali, R.; Strocchi, E.; Malini, P.; Casimirri, F.; Ambrosioni, E.; Melchionda, N.; Labo, G.

1985-07-01

378

Global structure of small Na clusters in different approaches  

Microsoft Academic Search

We compare the shapes of small Na clusters as they result from the jellium model, the cylindrically averaged pseudopotential scheme, and the distance-dependent tight-binding approach. Although these are three distinctively different approaches, we find a good agreement amongst the results, showing that all three models, taken in their appropriate domain of descriptive power, give an equally reliable picture of metal

F. Spiegelmann; R. Poteau; B. Montag; P.-G. Reinhard

1998-01-01

379

Purification of the cardiac Na+-Ca2+ exchange protein.  

PubMed

We have used fractionation procedures to enrich solubilized cardiac sarcolemma in the Na+-Ca2+ exchange protein. Sarcolemma is extracted with an alkaline medium to remove peripheral proteins and is then solubilized with decylmaltoside. Next, the exchanger is applied to DEAE-Sepharose and eluted with high salt. The DEAE fraction is applied to WGA-agarose, and a small fraction of protein, enriched in the exchanger, can be eluted by changing the detergent to Triton X-100. This fraction is reconstituted into asolectin proteoliposomes for measurement of Na+-Ca2+ exchange activity and gel electrophoresis. The purified fraction has a Na+-Ca2+ exchange activity of 600 nmol Ca2+/mg of protein per s at 10 microM Ca2+ and a purification factor of about 30 as compared with control reconstituted sarcolemmal vesicles. Ca2+-Ca2+ exchange and Na+-Ca2+ exchange activities were both present in the same final reconstituted vesicles indicating that the same protein is responsible for both transport activities. SDS-PAGE reveals two prominent protein bands at 70 and 120 kDa. After mild chymotrypsin treatment (1 microgram/ml), there is no loss of exchange activity, but the 120 kDa band disappears and the 70 kDa band becomes more dense. This suggests that the 70 kDa band is due to an active proteolytic fragment of the 120 kDa protein. Under non-reducing gel conditions, only a single protein band is seen with an apparent molecular weight of 160 kDa. Antibodies to the purified exchanger preparation are able to immunoprecipitate exchange activity and confirm that the 70 kDa protein derives from the 120 kDa protein. We propose that both the 70 and 120 kDa proteins are associated with the Na+-Ca2+ exchanger. PMID:3191125

Philipson, K D; Longoni, S; Ward, R

1988-11-22

380

Surface color centers on NaCl(001)  

NASA Astrophysics Data System (ADS)

Charging effects and probe induced damage usually render impossible the investigation of ionic crystal surfaces by means of many of the conventional surface science tools, like e.g. low-energy electron diffraction (LEED). A way out of this dilemma is using low current densities on surfaces of thin epitaxial insulator films grown on a conducting substrate. We studied the creation of color centers in and on epitaxial NaCl on Ge(001) by means of ELS-LEED, a unique combination of high resolution LEED and energy loss spectroscopy (ELS). Surface color centers determine the chemical reactivity of the NaCl(001) surface as will be demonstrated for the adsorption of water. - Different kinetics of formation permit the identification of different types of surface color centers. At temperatures below 200 K prolonged low-energy electron bombardment creates predominantly surface F- and M-centers appearing as randomly distributed static point defects. Only at room temperature does diffusion lead to the formation of Na clusters and colloids. When water is present anion vacancies are transformed into OH^--centers. During the formation of the two-dimensional condensed phase of H_2O on NaCl(001), OH^--centers act as nucleation centers for H_2O clusters. These limit the long-range order of the 2D-phase, reducing the superstructure from a c(2x12) symmetry as formed on annealed NaCl films to c(2x4). As a model we present an ice-like bilayer with a vertical modulation.

Zielasek, V.; Hildebrandt, T.; Henzler, M.

1998-03-01

381

Stoichiometric Relationship between Na+ Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of 23Na and 13C NMR Time Course Data  

PubMed Central

We examined the response of Na+,K+-ATPase (NKA) to monensin, a Na+ ionophore, with and without ouabain, an NKA inhibitor, in suspensions of human erythrocytes (red blood cells). A combination of 13C and 23Na NMR methods allowed the recording of intra- and extracellular Na+, and 13C-labeled glucose time courses. The net influx of Na+ and the consumption of glucose were measured with and without NKA inhibited by ouabain. A Bayesian analysis was used to determine probability distributions of the parameter values of a minimalist mathematical model of the kinetics involved, and then used to infer the rates of Na+ transported and glucose consumed. It was estimated that the numerical relationship between the number of Na+ ions transported by NKA per molecule of glucose consumed by a red blood cell was close to the ratio 6.0:1.0, agreeing with theoretical prediction. PMID:23601315

Puckeridge, Max; Chapman, Bogdan E.; Conigrave, Arthur D.; Grieve, Stuart M.; Figtree, Gemma A.; Kuchel, Philip W.

2013-01-01

382

Stoichiometric relationship between Na(+) ions transported and glucose consumed in human erythrocytes: Bayesian analysis of (23)Na and (13)C NMR time course data.  

PubMed

We examined the response of Na(+),K(+)-ATPase (NKA) to monensin, a Na(+) ionophore, with and without ouabain, an NKA inhibitor, in suspensions of human erythrocytes (red blood cells). A combination of (13)C and (23)Na NMR methods allowed the recording of intra- and extracellular Na(+), and (13)C-labeled glucose time courses. The net influx of Na(+) and the consumption of glucose were measured with and without NKA inhibited by ouabain. A Bayesian analysis was used to determine probability distributions of the parameter values of a minimalist mathematical model of the kinetics involved, and then used to infer the rates of Na(+) transported and glucose consumed. It was estimated that the numerical relationship between the number of Na(+) ions transported by NKA per molecule of glucose consumed by a red blood cell was close to the ratio 6.0:1.0, agreeing with theoretical prediction. PMID:23601315

Puckeridge, Max; Chapman, Bogdan E; Conigrave, Arthur D; Grieve, Stuart M; Figtree, Gemma A; Kuchel, Philip W

2013-04-16

383

Synthesis of Li (x)Na (2-x)Mn 2S 3 and LiNaMnS 2 through redox-induced ion exchange reactions  

NASA Astrophysics Data System (ADS)

Na 2Mn 2S 3 was oxidatively deintercalated using iodine in acetonitrile to yield Na 1.3Mn 2S 3, with lattice constants nearly identical to that of the reactant. Lithium was then reductively intercalated into the oxidized product to yield Li 0.7Na 1.3Mn 2S 3. When heated, this metastable compound decomposed to form a new crystalline compound, LiNaMnS 2, along with MnS and residual Na 2Mn 2S 3. Single crystal X-ray diffraction structural analysis of LiNaMnS 2 revealed that this compound crystallizes in P-3 m1 with cell parameters a=4.0479(6) Å, c=6.7759(14) Å, V=96.15(3) Å 3 ( Z=1, w R2=0.0367) in the NaLiCdS 2 structure-type.

Luthy, Joshua A.; Goodman, Phillip L.; Martin, Benjamin R.

2009-03-01

384

Two novel Zintl compounds Na 12Ge 8Sn and Na 15Ge 8SnP: Single crystal and electronic structures  

NASA Astrophysics Data System (ADS)

Two mixed anionic Zintl phases, Na 12Ge 8Sn and Na 15Ge 8SnP, have been synthesized and structurally characterized. Na 12Ge 8Sn crystallizes in the tetragonal space group P4 12 12 (#92, a = 14.1172(2) Å, c = 21.8483(7) Å, V = 4354.3(2) Å 3, Z = 8, and R/ wR = 0.0481/0.1213) and Na 15Ge 8SnP crystallizes in the orthorhombic space group Cmcm (#63, a = 17.517(4) Å, b = 12.890(3) Å, c = 11.111(2) Å, V = 2508.7(9) Å 3, Z = 4, and R/ wR = 0.0313/0.0669). Both compounds adopt new structure types and are made up of isolated Na + cations, anionic (Ge 4) 4- tetrahedral clusters, and Sn 4- or P 3- anions. The structure of Na 12Ge 8Sn can be described as a square packing of two kinds of columns, highly distorted Sn centered Na icosahedra chains ([NaSn]5+?1) and highly distorted Na(1) centered anti-rectangular prism (four (Ge 4) 4- and four Na, [Na()2]5-?1) chains. The structure of Na 15Ge 8SnP is composed of cationic [NaSnP]8+?1 saw tooth-like chains, which are made up of Sn centered Na bicapped hexagonal prisms and P centered tricapped trigonal prisms, extending along the c axis with anionic (Ge 4) 4- tetrahedral clusters between the chains. The electronic structures have been studied using density functional theory.

Dong, Yongkwan; Ranjan, Chinmoy; DiSalvo, Francis J.

2008-05-01

385

Na-O Anticorrelation and Horizontal Branches. V. The Na-O anticorrelation in NGC 6441 from Giraffe spectra  

E-print Network

We present an analysis of FLAMES-Giraffe spectra for several bright giants in NGC 6441, to investigate the presence and extent of the Na-O anticorrelation in this anomalous globular cluster. The field of NGC 6441 is very crowded, with severe contamination by foreground (mainly bulge) field stars. Appropriate membership criteria were devised to identify a group of 25 likely cluster members among the about 130 stars observed. Combined with the UVES data obtained with the same observations, high dispersion abundance analyses are now available for a total of 30 stars in NGC 6441, 29 of them having data for both O and Na. The spectra were analyzed by a standard line analysis procedure; care was taken to minimize the impact of the differential interstellar reddening throughout the cluster, and to extract reliable information from crowded, and moderately high S/N (30-70), moderately high resolution (R ~ 23,000) spectra. NGC 6441 is very metal-rich ([Fe/H]=$-0.34\\pm 0.02\\pm0.04$ dex). There is no clear sign of star-to-star scatter in the Fe-peak elements. The alpha-elements Mg, Si, Ca, and Ti are overabundant by rather large factors, suggesting that the cluster formed from material enriched by massive core collapse SNe. The O-Na anticorrelation is well defined, with about 1/4 of the stars being Na-rich and O-poor. One of the stars is a Ba-rich and moderately C-rich star. The distribution of [Na/O] ratios among RGB stars in NGC 6441 appears similar to the distribution of colors of stars along the horizontal branch. The fraction of Na-poor, O-rich stars found in NGC 6441 agrees well with that of stars on the red horizontal branch of this cluster (in both cases about 80%), with a sloping distribution toward lower values of [O/Na] (among RGB stars and bluer colors (among HB stars).

R. G. Gratton; S. Lucatello; A. Bragaglia; E. Carretta; S. Cassisi; Y. Momany; E. Pancino; E. Valenti; V. Caloi; R. Claudi; F. D'Antona; S. Desidera; P. Fran{ç}ois; G. James; S. Moehler; S. Ortolani; L. Pasquini; G. Piotto; A. Recio-Blanco

2007-01-08

386

Na-O anticorrelation and horizontal branches. V. The Na-O anticorrelation in NGC 6441 from Giraffe spectra  

NASA Astrophysics Data System (ADS)

Aims:We present an analysis of FLAMES-Giraffe spectra for several bright giants in NGC 6441, to investigate the presence and extent of the Na-O anticorrelation in this anomalous globular cluster. Methods: The field of NGC 6441 is very crowded, with severe contamination by foreground (mainly bulge) field stars. Appropriate membership criteria were devised to identify a group of 25 likely cluster members among the about 130 stars observed. Combined with the UVES data obtained with the same observations (Gratton et al. 2006), high dispersion abundance analyses are now available for a total of 30 stars in NGC 6441, 29 of them having data for both O and Na. The spectra were analyzed by a standard line analysis procedure; care was taken to minimize the impact of the differential interstellar reddening throughout the cluster, and to extract reliable information from crowded, and moderately high S/N (30-70), moderately high resolution (R˜ 23 000) spectra. Results: NGC 6441 has the typical abundance pattern seen in several other globular clusters. It is very metal-rich ([Fe/H] = -0.34 ± 0.02 ± 0.04 dex). There is no clear sign of star-to-star scatter in the Fe-peak elements. The ?-elements Mg, Si, Ca, and Ti are overabundant by rather large factors, suggesting that the cluster formed from material enriched by massive core collapse SNe. The O-Na anticorrelation is well defined, with about 1/4 of the stars being Na-rich and O-poor. One of the stars is a Ba-rich and moderately C-rich star. Such stars are rare in globular clusters. Conclusions: .The distribution of [Na/O] ratios among RGB stars in NGC 6441 appears similar to the distribution of colors of stars along the horizontal branch. The fraction of Na-poor, O-rich stars found in NGC 6441 agrees well with that of stars on the red horizontal branch of this cluster (in both cases about 80%), with a sloping distribution toward lower values of [O/Na] (among RGB stars) and bluer colors (among HB stars). Based on data collected at the European Southern Observatory with the VLT-UT2, Paranal, Chile (ESO Program 073.D-0211). Full Table [see full text] is only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/cgi-bin/qcat?J/A+A/464/953

Gratton, R. G.; Lucatello, S.; Bragaglia, A.; Carretta, E.; Cassisi, S.; Momany, Y.; Pancino, E.; Valenti, E.; Caloi, V.; Claudi, R.; D'Antona, F.; Desidera, S.; François, P.; James, G.; Moehler, S.; Ortolani, S.; Pasquini, L.; Piotto, G.; Recio-Blanco, A.

2007-03-01

387

Energetics of phosphate frameworks containing zinc and cobalt: NaZnPO 4, NaH(ZnPO 4) 2, NaZnPO 4·H 2O, NaZnPO 4· {4}/{3}H 2O, and NaCo xZn 1-xPO 4· {4}/{3}H 2O  

NASA Astrophysics Data System (ADS)

Differential scanning calorimetry and high temperature oxide melt solution calorimetry were used to study the enthalpy of the ?- ? phase transformation of NaZnPO 4 and enthalpies of formation of ?-NaZnPO 4, NaH(ZnPO 4) 2, NaZnPO 4·H 2O, and NaCo xZn 1-xPO 4· {4}/{3}H 2O ( x=0, 0.1, 0.2, 0.3). The enthalpies of formation from the oxides of cobalt substituted in NaZnPO 4· {4}/{3}H 2O do not depend on cobalt content, confirming similar acid-base interactions for Zn-PO 4 and Co-PO 4. While water molecules stabilize zinc phosphate frameworks through solvating a cation or forming extra hydrogen bonds, the partial substitution of water for sodium oxide to form NaH(ZnPO 4) 2 represents the formation of an acidic compound with weaker acid-base interactions and less exothermic enthalpy of formation from oxides than Na 2(ZnPO 4) 2.

Le, So-Nhu; Navrotsky, Alexandra

2007-09-01

388

Quantum mechanical calculation of 23 Na NMR shieldings in silicates and aluminosilicates  

Microsoft Academic Search

To assist in the assignment and interpretation of 23Na NMR spectra in silicate and aluminosilicate minerals and glasses we have calculated the 23Na NMR shieldings and the electric field gradients (EFG) at the Na for a number of Na-containing species. Included are Na(OH2)\\u000a n\\u000a \\u000a +, n?=?1, 2, 4, 5, 6 and 8, and Na+ complexes with SiH3OH, SiH3ONa and O(SiH3)2.

J. A. Tossell

1999-01-01

389

INTEX-NA: Intercontinental Chemical Transport Experiment - North America  

NASA Technical Reports Server (NTRS)

INTEX-NA is an integrated atmospheric chemistry field experiment to be performed over North America using the NASA DC-8 and P-3B aircraft as its primary platforms. It seeks to understand the exchange of chemicals and aerosols between continents and the global troposphere. The constituents of interest are ozone and its precursors (hydrocarbons, NOX and HOX), aerosols, and the major greenhouse gases (CO2, CH4, N2O). INTEX-NA will provide the observational database needed to quantify inflow, outflow, and transformations of chemicals over North America. INTEX-NA is to be performed in two phases. Phase A will take place during the period of May-August 2004 and Phase B during March-June 2006. Phase A is in summer when photochemistry is most intense and climatic issues involving aerosols and carbon cycle are most pressing, and Phase B is in spring when Asian transport to North America is at its peak. INTEX-NA will coordinate its activities with concurrent measurement programs including satellites (e. g. Terra, Aura, Envisat), field activities undertaken by the North American Carbon Program (NACP), and other U.S. and international partners. However, it is being designed as a 'stand alone' mission such that its successful execution is not contingent on other programs. Synthesis of the ensemble of observation from surface, airborne, and space platforms, with the help of global/regional models is an important It is anticipated that approximately 175 flight hours for each of the aircraft (DC-8 and P-3B) will be required for each Phase. Principal operational sites are tentatively selected to be Bangor, ME; Wallops Island, VA; Seattle, WA; Rhinelander, WI; Lancaster, CA; and New Orleans, LA. These coastal and continental sites can support large missions and are suitable for INTEX-NA objectives. The experiment will be supported by forecasts from meteorological and chemical models, satellite observations, surface networks, and enhanced O3,-sonde releases. In addition to characterizing Atlantic-outflow and Pacific-inflow, INTEX-NA will characterize air masses transported between the U.S., Canada, and Mexico. INTEX-NA will be the first continental scale inflow, outflow, and transformation experiment to be performed over North America. It will provide the most comprehensive observational data set to date to understand the O3/NOX/HOX/aerosol photochemical system and the carbon cycle. One of the critical needs of the carbon cycle research is to obtain large-scale vertical and horizontal concentration gradients of CO2, throughout the troposphere over continental source/sink regions. INTEX-NA is ideally suited to perform this role. Coastal and continental operational sites will allow us to develop a curtain profile of greenhouse gases (e. g. CO2,) and other key pollutants across North America. Such information is central to our quantitative understanding of chemical budgets on the continental scale. We expect to provide a number of satellite under-flights over land and water to test and validate observations from the appropriate satellite platform (e. g. Aura). We plan to develop strong collaborations with other national and international observational programs. Results from INTEX-NA should directly benefit the development of environmental policy for air quality and climate change.

Singh, Hanwant B.; Jacob, D.; Pfister, L.; Hipskind, R. Stephen (Technical Monitor)

2002-01-01

390

Stabilization of Na,K-ATPase by ionic interactions.  

PubMed

The effect of ions on the thermostability and unfolding of Na,K-ATPase from shark salt gland was studied and compared with that of Na,K-ATPase from pig kidney by using differential scanning calorimetry (DSC) and activity assays. In 1 mM histidine at pH 7, the shark enzyme inactivates rapidly at 20 degrees C, as does the kidney enzyme at 42 degrees C (but not at 20 degrees C). Increasing ionic strength by addition of 20 mM histidine, or of 1 mM NaCl or KCl, protects both enzymes against this rapid inactivation. As detected by DSC, the shark enzyme undergoes thermal unfolding at lower temperature (Tm approximately 45 degrees C) than does the kidney enzyme (Tm approximately 55 degrees C). Both calorimetric endotherms indicate multi-step unfolding, probably associated with different cooperative domains. Whereas the overall heat of unfolding is similar for the kidney enzyme in either 1 mM or 20 mM histidine, components with high mid-point temperatures are lost from the unfolding transition of the shark enzyme in 1 mM histidine, relative to that in 20 mM histidine. This is attributed to partial unfolding of the enzyme due to a high hydrostatic pressure during centrifugation of DSC samples at low ionic strength, which correlates with inactivation measurements. Addition of 10 mM NaCl to shark enzyme in 1 mM histidine protects against inactivation during centrifugation of the DSC sample, but incubation for 1 h at 20 degrees C prior to addition of NaCl results in loss of components with lower mid-point temperatures within the unfolding transition. Cations at millimolar concentration therefore afford at least two distinct modes of stabilization, likely affecting separate cooperative domains. The different thermal stabilities and denaturation temperatures of the two Na,K-ATPases correlate with the respective physiological temperatures, and may be attributed to the different lipid environments. PMID:18187035

Fodor, Elfrieda; Fedosova, Natalya U; Ferencz, Csilla; Marsh, Derek; Pali, Tibor; Esmann, Mikael

2008-04-01

391

MOŽNOSTI ZDRAVLJENJA KLIMAKTERI?NIH TEŽAV PRI BOLNICAH Z RAKOM DOJK TREATMENT POSSIBILITIES OF MENOPAUSAL SYMPTOMS IN BREAST CANCER PATIENTS  

Microsoft Academic Search

Izvle?ek Izhodiš?a Rak dojk je najpogostejši rak pri ženskah. Klimakteri?ne težave, ki zelo poslabšajo kakovost življenja pri ženskah vseh starosti, so pogost sopojav sistemskega zdravljenja, tako hormon- skega zdravljenja kot zdravljenja s citostatiki. Metode V prispevku je predstavljen pregled literature o vzrokih, na?inu obravnave in možnostih zdravljenja klimakteri?nih težav pri bolnicah z rakom dojk. Rezultati Za lajšanje vazomotornih simptomov obstaja

Darja Arko

392

[Effect of NaHCO3 stress on uptake and transportation of Na+, K+ and Ca2+ in three shrub species].  

PubMed

We detected absorption and transportation of ions in the leaves of Atriplex nummularia, Atriplex canescens and Lycium barbarum under NaHCO3 stress (300 mmol x L(-1)) by using atomic absorption spectrophotometry and non-invasive ion flux measurement. The results showed that leaves of the A. nummularia, A. canescens and L. barbarum exhibited a high capacity to induce the Na+ accumulation when compared with that of control. The higher the concentration of NaHCO3 treatment, the more Na+ accumulated in the leaves of the three plants under experimental condition. L. barbarum showed a higher Na+ efflux in the mesophyll cells, whereas A. nummularia and A. canescens showed a relative lower efflux. A lower K+ content and a higher Na+/K+ ratio were detected in leaves of A. nummularia and L. barbarum. However, a higher K+ content and a lower Na+/K+ ratio were seen in leaves of A. canescens. Due to induction of Ca2+ efflux under the NaHCO3 treatment, a lower Ca2+ content and a higher Na+/Ca2+ ratio were observed in L. barbarum. On the contrary, a higher Ca2+ influx was observed in A. nummularia and A. canescens. These results suggested that the three shrubs species had different Na+ segmentation strategies. The accumulation of Na+ inhibited Ca2+ absorption in leaves of L. barbarum, while in the A. nummularia and A. canescens, Ca2+ influx induced [Ca2+]cyt which preserved a less-depolarized PM and then inhibited K efflux. The maintaining of cellular K+/Na+ homeostasis in A. nummularia and A. canescens might be achieved by the induction of [Ca2+]cyt under the NaHCO3 treatment. PMID:24984488

Mao, Gui-Lian; Li, Guo-Qi; Xu, Xing; Zhang, Xin-Xue

2014-03-01

393

Performance comparison of MoNA and LISA neutron detectors  

NASA Astrophysics Data System (ADS)

In 2002 eight primarily undergraduate institutions constructed and tested the Modular Neutron Array (MoNA) which has been used to detect high energy neutrons at the National Superconducting Cyclotron Laboratory (NSCL). Nine institutions have now designed, constructed and tested the Large-area multi-Institutional Scintillator Array (LISA) neutron detector which will be used at the NSCL and the future Facility for Rare Isotope Beams (FRIB). Both detectors are comprised of 144 detector modules. Each module is a 200 x 10 x 10 cm^3 bar organic plastic scintillator with a photomultiplier tube mounted on each end. Using cosmic rays and a gamma source, we compared the performance of MoNA and LISA by using the same electronics to check light attenuation, position resolution, rise times, and cosmic ray peak widths. Results will be presented.

Purtell, Kimberly; Rethman, Kaitlynne; Haagsma, Autumn; Finck, Joseph; Smith, Jenna; Snyder, Jesse

2010-11-01

394

The Offline Software Framework of the NA61/SHINE Experiment  

NASA Astrophysics Data System (ADS)

NA61/SHINE (SHINE = SPS Heavy Ion and Neutrino Experiment) is an experiment at the CERN SPS using the upgraded NA49 hadron spectrometer. Among its physics goals are precise hadron production measurements for improving calculations of the neutrino beam flux in the T2K neutrino oscillation experiment as well as for more reliable simulations of cosmic-ray air showers. Moreover, p+p, p+Pb and nucleus+nucleus collisions will be studied extensively to allow for a study of properties of the onset of deconfinement and search for the critical point of strongly interacting matter. Currently NA61/SHINE uses the old NA49 software framework for reconstruction, simulation and data analysis. The core of this legacy framework was developed in the early 1990s. It is written in different programming and scripting languages (C, pgi-Fortran, shell) and provides several concurrent data formats for the event data model, which includes also obsolete parts. In this contribution we will introduce the new software framework, called Shine, that is written in C++ and designed to comprise three principal parts: a collection of processing modules which can be assembled and sequenced by the user via XML files, an event data model which contains all simulation and reconstruction information based on STL and ROOT streaming, and a detector description which provides data on the configuration and state of the experiment. To assure a quick migration to the Shine framework, wrappers were introduced that allow to run legacy code parts as modules in the new framework and we will present first results on the cross validation of the two frameworks.

Sipos, Roland; Laszlo, Andras; Marcinek, Antoni; Paul, Tom; Szuba, Marek; Unger, Michael; Veberic, Darko; Wyszynski, Oskar

2012-12-01

395

Na,K-ATPase: Isoform structure, function, and expression  

Microsoft Academic Search

An interesting feature of the Na,K-ATPase is the multiplicity of a and ß isoforms. Three isoforms exist for the a subunit, a1, a2, and a3, as well for the ß subunit, ß1, ß2, and ß3. The functional significance of these isoforms is unknown, but they are expressed in a tissue- and developmental-specific manner. For example, all three isoforms of the

Jerry B. Lingrel

1992-01-01

396

Background understanding and improvement in NaI scintillators  

NASA Astrophysics Data System (ADS)

Sodium Iodide scintillators are very interesting particle and radiation detectors. Low background requirements could limit their application in a variety of fields. We report the e.orts to understand and reduce the background in a set of 14 NaI detectors, stored underground since 1988, undertaken in the frame of the ANAIS experiment [1] in several directions, including PSD techniques, Monte Carlo simulations and detector upgrading.

Amaré, J.; Beltrán, B.; Carmona, J. M.; Cebrián, S.; García, E.; Gómez, H.; Irastorza, I. G.; Luzón, G.; Martínez, M.; Morales, J.; Ortiz de Solórzano, A.; Pobes, C.; Puimedón, J.; Rodríguez, A.; Ruz, J.; Sarsa, M. L.; Torres, L.; Villar, J. A.

2006-05-01

397

Radiation-induced creep of confined NaCI  

Microsoft Academic Search

Samples of pure single crystals of NaC1 and of a natural rock salt were simultaneously gamma-irradiated at an approximate constant dose rate (15 kGy\\/h) and a constant temperature (100°C) and for different lengths of time, resulting in integrated doses ranging from 0.02 to 44.6 MGy. Microstructural analysis showed that both incipient deformation structures and very advanced recovery structures develop during

A. García Celma; H. Donker

1994-01-01

398

Mode coupling in low NA plastic optical fibers  

NASA Astrophysics Data System (ADS)

Using the power flow equation and experimental measurements, investigated in this article is the state of mode coupling in low NA (0.3) step-index plastic optical fibers for laser beam launch. Results show the coupling length Lc at which the equilibrium mode distribution is achieved and the length zs of the fiber required for achieving the steady-state mode distribution. Numerical results obtained using the power flow equation agree well with experimental measurements.

Savovi?, Svetislav; Kova?evi?, Milan S.; Djordjevich, Alexandar; Baji?, Jovan S.; Stupar, Dragan Z.; Stepniak, Grzegorz

2014-08-01

399

Third-Order Elastic Constants of NaF  

Microsoft Academic Search

The complete set of third-order elastic constants for single crystals of NaF was determined from the hydrostatic-pressure and uniaxial-stress derivatives of natural sound velocities measured with a two-crystal ultrasonic-interference technique. There appeared to be no dislocation motion during the uniaxial measurements since changes in the natural sound velocities with stress were linear, and data recorded before and after a gamma

W. A. Bensch

1972-01-01

400

Two-frequency lidar technique for mesospheric Na temperature measurements  

Microsoft Academic Search

The authors describe a new two-frequency lidar for measuring Na temperature profiles that uses a stabilized cw single-mode dye laser oscillator (rms frequency jitter < 1 MHz) followed by a pulsed-dye power amplifier (140 MHz FWHM linewidth) which is pumped by an injection-locked Nd:YAG laser. The laser oscillator is tuned to the two operating frequencies by observing the Doppler-free structure

C. Y. She; H. Latifi; J. R. Yu; R. J. II Alvarez; R. E. Bills; C. S. Gardner

1990-01-01

401

NaCl-induced accelerated oxidation of a titanium alloy  

Microsoft Academic Search

The effect of a surface deposit of NaCl on the oxidation characteristics of a high-temperature titanium alloy was studied in the temperature range of 400–600°C. The influence of the water vapor content in the oxidizing environment was examined in the light of an existing electrochemical mechanism proposed for the hot-salt stress-corrosion-cracking phenomenon found in titanium alloys. It was established that

P. Dumas; C. St. John

1976-01-01

402

Na+ channel aggregation in remyelinating mouse sciatic axons following transection.  

PubMed

Mouse sciatic nerves from the degeneration-resistant strain C57BL/6/Wld (Ola) were surgically injected with lysolecithin to induce focal demyelination. Three days later they were transected adjacent to the spinal cord to eliminate contact of the axons with their cell bodies. The Na+ channel distribution was assessed by immunocytochemistry and followed at several stages of remyelination. Control experiments were performed on nerves that were injected but not cut. At (3 + 4) days, namely, nerves cut 3 days post-injection and examined 4 days after cutting, axons contained fully demyelinated regions. Na+ channel clusters appeared only at heminodes and at isolated sites that are likely to represent original nodes of Ranvier. During the next few days proliferating Schwann cells adhered to the axons and extended their processes. Clusters of Na+ channels appeared at their edges, and as the Schwann cells elongated the distance between these aggregates increased. A few clusters associated with neighboring Schwann cells approached each other and appeared to coalesce at sites where presumably new nodes of Ranvier would be formed. Beyond (3 + 6) days excessive degeneration of the transected axons precluded further observations. In the uncut controls, the spatio-temporal sequence of Schwann cell proliferation and channel patch formation and movement was similar to that described above, although myelin formation was somewhat faster than in the cut axons. It is concluded that Na+ channel aggregation associated with the early stages of remyelination is not dependent upon continuous communication of the axon with its cell body and is under local control. PMID:8567070

Tzoumaka, E E; Novakovi?, S D; Levinson, S R; Shrager, P

1995-10-01

403

Uptake and reaction of HOBr on frozen and dry NaCl/NaBr surfaces between 253 and 233 K  

NASA Astrophysics Data System (ADS)

The uptake and reaction of HOBr with frozen salt surfaces of variable NaCl / NaBr composition and temperature were investigated with a coated wall flow tube reactor coupled to a mass spectrometer for gas-phase analysis. HOBr is efficiently taken up onto the frozen surfaces at temperatures between 253 and 233 K where it reacts to form the di-halogens BrCl and Br2, which are subsequently released into the gas-phase. The uptake coefficient for HOBr reacting with a frozen, mixed salt surface of similar composition to sea-spray was 10-2. The relative concentration of BrCl and Br2 released to the gas-phase was found to be strongly dependent on the ratio of Cl- to Br - in the solution prior to freezing / drying. For a mixed salt surface of similar composition to sea-spray the major product at low conversion of surface reactants (i.e. Br - and Cl-) was Br2. Variation of the pH of the NaCl / NaBr solution used to prepare the frozen surfaces was found to have no significant influence on the results. The observations are explained in terms of initial formation of BrCl in a surface reaction of HOBr with Cl-, and conversion of BrCl to Br2 via reaction of surface Br -. Experiments on the uptake and reaction of BrCl with frozen NaCl / NaBr solutions served to confirm this hypothesis. The kinetics and products of the interactions of BrCl, Br2 and Cl2 with frozen salt surfaces were also investigated, and lower limits to the uptake coefficients of > 0.034, >0.025 and >0.028 respectively, were obtained. The uptake and reaction of HOBr on dry salt surfaces was also investigated and the results closely resemble those obtained for frozen surfaces. During the course of this study the gas diffusion coefficients of HOBr in He and H2O were also measured as (273 ± 1) Torr cm2 s-1 and (51 ± 1) Torr cm2 s-1, respectively, at 255 K. The implications of these results for modelling the chemistry of the Arctic boundary layer in springtime are discussed.

Adams, J. W.; Holmes, N. S.; Crowley, J. N.

2002-04-01

404

Grant Title: POSTDOCTORAL FELLOWSHIP IN MENTAL HEALTH AND SUBSTANCE ABUSE SERVICES Funding Opportunity Number: NA  

E-print Network

Grant Title: POSTDOCTORAL FELLOWSHIP IN MENTAL HEALTH AND SUBSTANCE ABUSE SERVICES (MHSAS) Funding Opportunity Number: NA Agency/Department: American Psychological Association (APA), Substance Abuse and Mental health and substance abuse services and research. Release and Expiration: NA Application Deadline

Farritor, Shane

405

Factors influencing skeletal muscle Na+, K+-ATPase and Plasma [K+] during exercise in humans.  

E-print Network

??The sodium-potassium adenosine triphosphatase enzyme (Na+,K+ -ATPase) regulates muscle cell [Na+],[K+] and muscle membrane potential, therefore playing a fundamental role in muscle excitability. This dissertation… (more)

Fraser, Steven Francis

2000-01-01

406

Preferential Interaction of Na+ over K+ to Carboxylate-functionalized Silver Nanoparticles  

EPA Science Inventory

Elucidating mechanistic interactions between specific ions (Na+/ K+) and nanoparticle surfaces to alter particle stability in polar media has received little attention. We investigated relative preferential binding of Na+ and K+ to carboxylate-functionalized silver nanoparticles ...

407

Subunit Dependence of Na Channel Slow Inactivation and Open Channel Block in Cerebellar Neurons  

E-print Network

inactivation, slow inactivation, and open-channel block, we recorded voltage-clamped, tetrodotoxin-sensitive Na firing depends on specialized voltage-gated channels. These include tetrodotoxin (TTX)- sensitive Na

Raman, Indira M.

408

Solvation of Na+, K+, and their dimers in helium.  

PubMed

Helium atoms bind strongly to alkali cations which, when embedded in liquid helium, form so-called snowballs. Calculations suggest that helium atoms in the first solvation layer of these snowballs form rigid structures and that their number (n) is well defined, especially for the lighter alkalis. However, experiments have so far failed to accurately determine values of n. We present high-resolution mass spectra of Na(+)He(n), K(+)He(n), Na(2)(+)He(n) and K(2)(+)He(n), formed by electron ionization of doped helium droplets; the data allow for a critical comparison with several theoretical studies. For sodium and potassium monomers the spectra indicate that the value of n is slightly smaller than calculated. Na(2)(+)He(n) displays two distinct anomalies at n=2 and n=6, in agreement with theory; dissociation energies derived from experiment closely track theoretical values. K(2)(+)He(n) distributions are fairly featureless, which also agrees with predictions. PMID:22374575

An der Lan, Lukas; Bartl, Peter; Leidlmair, Christian; Jochum, Roland; Denifl, Stephan; Echt, Olof; Scheier, Paul

2012-04-01

409

The local distribution of Na I interstellar gas  

NASA Technical Reports Server (NTRS)

We present high-resolution absorption measurements (lambda/Delta lambda approximately 75,000) of the interstellar Na I D lines at 5890 A toward 80 southern hemisphere early-type stars located in the local interstellar medium (LISM). Combining these results with other sodium measurements taken from the literature, we produce galactic maps of the distribution of neutral sodium column density for a total of 293 stars generally lying within approximately 250 pc of the Sun. These maps reveal the approximate shape of the mid-plane contours of the rarefied region of interstellar space termed the Local Bubble. Its shape is seen as highly asymmetric, with a radius ranging from 30 to 300 pc, and with an average radius of 60 pc. Similar plots of the Galactic mid-plane distribution of sources emitting extreme ultraviolet radiation show that they also trace out similar contours of the Local Bubble derived from Na I absorption measurements. We conclude that the Local Bubble absorption interface can be represented by a hydrogen column density, Nu(sub ETA) = 2 x 10(exp 19) cm(exp -2), which explains both the local distribution of Na I absorption and the observed galactic distribution of extreme ultraviolet sources. The derived mid-plane contours of the Bubble generally reproduce the large-scale features carved out in the interstellar medium by several nearby galactic shell structures.

Welsh, B. Y.; Craig, N.; Vedder, P. W.; Vallerga, J. V.

1994-01-01

410

Xylanase recovery by ethanol and Na2SO4 precipitation.  

PubMed

Xylans are the major components of the hemicellulosic fraction of lignocellulosic biomass and their hydrolysis can be obtained using xylanases from Penicillium janthinellum. In this work, sugarcane bagasse hemicellulosic hydrolysate was used as the substrate for producing xylanase. The precipitation of these enzymes was studied using ethanol and Na2SO4 as precipitating agents. Ethanol precipitation experiments were performed batchwise in concentrations ranging from 10 to 80%, pH 4.0 to 7.0, at 4 degrees C. The concentrations used in the precipitations with Na2SO4 were from 5 to 60% at pH 5.5 and 25 degrees C. Solubility curves as a function of xylanase activity and total protein for both precipitating agents were made. According to the results, Na2SO4 is not appropriate for precipitating xylanases in this medium since at salt concentrations higher than 25%, the enzyme was denaturated and at this concentration less than 80% of the enzyme and total protein were precipitated. Because of differences in xylanase and total protein solubility, a fractionated precipitation using ethanol can be performed, since with 40% ethanol, 49% of the total protein was precipitated and more than 95% of the enzyme was kept in solution. On the other hand approx 100% of the xylanases were recovered by precipitation after adding 80% ethanol. PMID:18576030

Cortez, E V; Pessoa, A; Assis, A N

1998-01-01

411

Aldosterone Regulates Na,K-ATPase and Increases Lung Edema Clearance in Rats  

Microsoft Academic Search

Aldosterone increases the Na,K-ATPase function in renal cells involved in active Na 1 transport. Be- cause the alveolar type 2 (AT2) cells participate in active Na 1 transport, we studied whether aldoster- one regulates the Na,K-ATPase in rat AT2 cells and whether aldosterone delivered by aerosols to spontaneously breathing rats affects edema clearance in a model of isolated-perfused lungs. The

WALTER G. OLIVERA; DAVID E. CICCOLELLA; NORA BARQUIN; KAREN M. RIDGE; DAVID H. RUTSCHMAN; DONOVAN B. YEATES; JACOB I. SZNAJDER

2000-01-01

412

Ion permeation in normal and batrachotoxin-modified Na+ channels in the squid giant axon  

Microsoft Academic Search

Na + permeation through normal and batrachotoxin (BTX)-modified squid axon Na ÷ channels was characterized. Unmodified and toxin-modified Na ÷ channels were studied simultaneously in outside-out membrane patches using the cut-open axon technique. Current-voltage relations for both normal and BTX- modified channels were measured over a wide range of Na ÷ concentrations and voltages. Channel conductance as a function of

ANA M. CORREA; RAMON LATORRE; FRANCISCO BEZANILLA

1991-01-01

413

The effect of NaCl on proline accumulation in rice leaves  

Microsoft Academic Search

The regulation of proline accumulation in detached leaves of rice(Oryza sativa cv. Taichung Native 1) was investigated.Increasing concentrations of NaCl from 50 to 200 mM progressivelyincreased proline content in detached rice leaves. NaCl induced prolineaccumulation was mainly due to the effect of both Na+ andCl- ions. Proline accumulation caused by NaCl was related toprotein proteolysis, an increase in ornithine-d-aminotransferaseactivity,a decrease

Chuan Chi Lin; Yi Ting Hsu; Ching Huei Kao

2002-01-01

414

Cryptosin induces backbone structural changes in cardiac Na+ and K+ dependent adenosinetriphosphatase.  

PubMed

Cryptosin, a new cardenolide, was found to preferentially bind to Na,K-ATPase enzyme (7), which is believed to be the ouabain binding site on cardiac sarcolemmal membrane. CD spectral studies revealed that cryptosin, in the presence of Na+ and Mg++ ions, bind to Na,K-ATPase and induce a dose-dependent change in the backbone structure of cardiac Na,K-ATPase. PMID:1965277

Venkateswara, R R; Dipak, D; Banning, J W; Vaidyanathan, C S

1990-10-01

415

Caustic Recycle from Hanford Tank Waste Using Large Area NaSICON Structures (LANS)  

SciTech Connect

This report presents the results of a 5-day test of an electrochemical bench-scale apparatus using a proprietary (NAS-GY) material formulation of a (Na) Super Ion Conductor (NaSICON) membrane in a Large Area NaSICON Structures (LANS) configuration. The primary objectives of this work were to assess system performance, membrane seal integrity, and material degradation while removing Na from Group 5 and 6 tank waste from the Hanford Site.

Fountain, Matthew S.; Sevigny, Gary J.; Balagopal, S.; Bhavaraju, S.

2009-03-31

416

Differential blockade of neuronal voltage-gated Na + and K + channels by antidepressant drugs  

Microsoft Academic Search

The effects of a range of antidepressants were investigated on neuronal voltage-gated Na+ and K+ channels. With the exception of phenelzine, all antidepressants inhibited batrachotoxin-stimulated 22Na+ uptake, most likely via negative allosteric inhibition of batrachotoxin binding to neurotoxin receptor site-2 on the Na+ channel. Imipramine also produced a differential action on macroscopic Na+ and K+ channel currents in acutely dissociated

Graham M Nicholson; Tim Blanche; Kylie Mansfield; Yvonne Tran

2002-01-01

417

Na8Au9.8(4)Ga7.2 and Na17Au5.87(2)Ga46.63: The diversity of pseudo 5-fold symmetries in the Na-Au-Ga system  

NASA Astrophysics Data System (ADS)

The Na-rich part (~30% Na) of the Na-Au-Ga system between NaAu2, NaGa4, and Na22Ga39 has been found to contain the ternary phases Na8Au9.8(4)Ga7.2 (I) and Na17Au5.87(2)Ga46.63 (II), according to the results of single crystal X-ray diffraction measurements. I is orthorhombic, Cmcm, a=5.3040(1), b=24.519(5), c=14.573(3) Å, and contains a network of clusters with local 5-fold symmetry along the a-axis. Such clusters are frequent building units in decagonal quasicrystals and their approximants. II is rhombohedral, R3¯m, a=16.325(2), c=35.242(7) Å, and contains building blocks that are structurally identical to the Bergman-type clusters as well as fused icosahedral units known with active metals, triels and late transition elements. II also contains a polycationic network with elements of the clathrate V type structure. Tight-binding electronic structure calculations using linear muffin-tin-orbital (LMTO) methods on idealized models of I and II indicate that both compounds are metallic with evident pseudogaps at the corresponding Fermi levels. The overall Hamilton bond populations are generally dominated by Au-Ga and Au-Au bonds in I and by Ga-Ga bonds in II; moreover, the Na-Au and Na-Ga contributions in I are unexpectedly large, ~20% of the total. A similar involvement of sodium in covalent bonding has also been found in the electron-richer i-Na13Au12Ga15 quasicrystal approximant.

Smetana, Volodymyr; Corbett, John D.; Miller, Gordon J.

2013-11-01

418

Na+-transport modulation induces isoform-specific expression of Na+,K+ATPase ?-subunit isoforms in C2C12 skeletal muscle cell  

Microsoft Academic Search

Changes in demands for Na+ transport alter expression of the Na+,K+-ATPase subunit isoforms. In skeletal muscle, the effects of these changes on expression the a2 isoform, the major isoform expressed in differentiated muscle cell, is not known. Therefore, this study examines regulation of the a-subunit isoforms by Na+ in the C2C12 skeletal muscle cell that expresses the a1 and a2

Richard Ladka; Yuk-Chow Ng

2000-01-01

419

ROS-mediated vascular homeostatic control of root-to-shoot soil Na delivery in Arabidopsis  

PubMed Central

Sodium (Na) is ubiquitous in soils, and is transported to plant shoots via transpiration through xylem elements in the vascular tissue. However, excess Na is damaging. Accordingly, control of xylem-sap Na concentration is important for maintenance of shoot Na homeostasis, especially under Na stress conditions. Here we report that shoot Na homeostasis of Arabidopsis thaliana plants grown in saline soils is conferred by reactive oxygen species (ROS) regulation of xylem-sap Na concentrations. We show that lack of A. thaliana respiratory burst oxidase protein F (AtrbohF; an NADPH oxidase catalysing ROS production) causes hypersensitivity of shoots to soil salinity. Lack of AtrbohF-dependent salinity-induced vascular ROS accumulation leads to increased Na concentrations in root vasculature cells and in xylem sap, thus causing delivery of damaging amounts of Na to the shoot. We also show that the excess shoot Na delivery caused by lack of AtrbohF is dependent upon transpiration. We conclude that AtrbohF increases ROS levels in wild-type root vasculature in response to raised soil salinity, thereby limiting Na concentrations in xylem sap, and in turn protecting shoot cells from transpiration-dependent delivery of excess Na. PMID:23064146

Jiang, Caifu; Belfield, Eric J; Mithani, Aziz; Visscher, Anne; Ragoussis, Jiannis; Mott, Richard; Smith, J Andrew C; Harberd, Nicholas P

2012-01-01

420

Potential curves for Na2/+/ and resonance charge transfer cross sections.  

NASA Technical Reports Server (NTRS)

A mode potential method, applied earlier to the positively charged diatomic lithium molecule Li2(+), is used to calculate the six lowest potential energy curves of Na2(+). Charge transfer cross sections are calculated for Li(+) on Li and for Na(+) on Na and found to be in reasonable agreement with experiment.

Bottcher, C.; Allison, A. C.; Dalgarno, A.

1971-01-01

421

On the locus of action of Na + at site I of oxidative phosphorylation  

Microsoft Academic Search

The effect of octylguanidine on the Na+ stimulated oxygen uptake of rat liver mitochondria and bovine heart submitochondrial particles and on the Na+ induced efflux of K from the mitochondria has been examined. The results indicate that the action of Na+ is inhibited by octylguanidine, but that the degree of inhibition depends on the concentration of the cation. Apparently, a

A. Gómez-Puyou; F. Sandoval; M. Tuena De Gómez-Puyou; E. Chávez; E. Pinto

1972-01-01

422

Expression of Cardiac Na Channels with Appropriate Physiological and Pharmacological Properties in Xenopus Oocytes  

Microsoft Academic Search

The objective of this study was to determine whether the Xenopus laevis oocyte can express an exogenous cardiac Na channel that retains its normal physiological and pharmacological properties. Cardiac Na channels were expressed in oocytes following injection of RNA from guinea pig, rat, and human heart and detailed analysis was performed for guinea pig cardiac Na channels. Average current amplitudes

D. S. Krafte; W. A. Volberg; K. Dillon; A. M. Ezrin

1991-01-01

423

Glycosaminoglycan polymerization may enable osmotically inactive Na+ storage in the skin.  

PubMed

Osmotically inactive skin Na(+) storage is characterized by Na(+) accumulation without water accumulation in the skin. Negatively charged glycosaminoglycans (GAGs) may be important in skin Na(+) storage. We investigated changes in skin GAG content and key enzymes of GAG chain polymerization during osmotically inactive skin Na(+) storage. Female Sprague-Dawley rats were fed a 0.1% or 8% NaCl diet for 8 wk. Skin GAG content was measured by Western blot analysis. mRNA content of key dermatan sulfate polymerization enzymes was measured by real-time PCR. The Na(+) concentration in skin was determined by dry ashing. Skin Na(+) concentration during osmotically inactive Na(+) storage was 180-190 mmol/l. Increasing skin Na(+) coincided with increasing GAG content in cartilage and skin. Dietary NaCl loading coincided with increased chondroitin synthase mRNA content in the skin, whereas xylosyl transferase, biglycan, and decorin content were unchanged. We conclude that osmotically inactive skin Na(+) storage is an active process characterized by an increased GAG content in the reservoir tissue. Inhibition or disinhibition of GAG chain polymerization may regulate osmotically inactive Na(+) storage. PMID:14975935

Titze, Jens; Shakibaei, Mehdi; Schafflhuber, Markus; Schulze-Tanzil, Gundula; Porst, Markus; Schwind, Karl H; Dietsch, Peter; Hilgers, Karl F

2004-07-01

424

Comparison of meteor radar and Na Doppler lidar measurements of winds in the mesopause region  

E-print Network

Comparison of meteor radar and Na Doppler lidar measurements of winds in the mesopause region above 29 January 2005. [1] Simultaneous sodium (Na) Doppler lidar and meteor radar measurements/s at altitudes below 96 km. This is smaller than the RMS differences observed in a previous Na lidar and meteor

Chu, Xinzhao

425

Elevated levels of glucose and L-fucose reduce 22Na+ uptake and whole cell Na+ current in cultured neuroblastoma cells.  

PubMed

Na+ flux was studied in cultured neuroblastoma cells grown in medium containing increased glucose or L-fucose concentrations. Chronic exposure of neuroblastoma cells to 30 mM glucose or 30 mM L-fucose caused a decrease in ouabain-sensitive and veratridine-stimulated 22Na+ uptake compared with cells cultured in unsupplemented medium. The Na+ current, determined by using whole-cell configuration of the patch clamp, was also decreased in these cells. Tetrodotoxin (3 microM), which blocked whole cell Na+ currents, also blocked veratridine-stimulated 22Na+ accumulation. Culturing cells in medium containing 30 mM fructose as an osmotic control had no effect on Na+ flux. Specific [3H]saxitoxin binding was not affected by 30 mM glucose or 30 mM L-fucose compared with cells grown in unsupplemented medium, suggesting that the number of Na+ channels was not decreased. These studies suggest that exposing cultured neuronal cells to conditions that occur in the diabetic milieu alters Na+ transport and Na(+)-channel activity. PMID:8263545

Yorek, M A; Stefani, M R; Wachtel, R E

1994-01-01

426

A study of the reaction NaO2 + O yields NaO + O2: Implications for the chemistry of sodium in the upper atmosphere  

SciTech Connect

Sodium superoxide (NaO2) is believed to be a major sink for meteor-ablated Na in the upper atmosphere. However, the rate constant for the reaction between NaO2 and O is not known, and its determination is the subject of this study. NaO2 was produced by the reaction between Na vapor, an excess of O2, and the carrier gas N2 in the upstream section of a fast flow tube reactor. Excess atomic O was then added, so that a steady state developed in which the sodium was partitioned between its atomic form and a variety of oxides. The steady state fraction of atomic Na, monitored by laser-induced fluorescence, was then observed as a function of (O) and (O2), from which it was deduced that k(NaO2 + O) = (2.2 +/- 1.0) x 10(exp -11) cu cm/molecule/s at 300 K (3 sigma uncertainty). As a prelude to this experiment, the recombination reaction between O2 and Na was studied by the conventional flow tube technique, yielding k(Na + O2 + N2) = (3.14 +/- 0.20) x 10(exp -30)(300/T)(exp -1.52 +/- 0.27)cm(exp 6)/molecule(exp 2)/s. These experimental results were then incorporated into a one-dimensional model of sodium in the upper atmosphere between 65 and 110 km. It is shown that formation of NaHCO3 rather than NaO2 is the dominant removal process for atomic Na below 90 km. This new model, whole only important assumption is the rate constant for the reaction NaHCO3 + H yields Na + H2CO3, predicts a seasonal variation of the atomic Na layer in excellent agreement with recent lidar observations at 40 deg and 69 deg N and also demonstrates that temperature fluctuations produced by gravity waves should induce significant chemical responses in the Na layer below 88 km.

Helmer, M.; Plane, J.M.C. [Univ. of East Anglia, Norwich (United Kingdom)

1993-12-01

427

Seasonal variations of midlatitude mesospheric Na layer and their tidal period perturbations based on full diurnal cycle Na lidar observations of 2002-2008  

NASA Astrophysics Data System (ADS)

The Na lidar facility at Colorado State University (41°N, 105°W) started the full diurnal cycle observations of mesopause region temperature and zonal and meridional winds as well as mesospheric Na density in May 2002. In this paper, monthly means and seasonal variations of the density of mesospheric Na based on lidar observations from May 2002 to December 2008 are reported along with the amplitude and phase of tidal period perturbations. The revealed seasonal behaviors of mesospheric Na layer are generally consistent with published nocturnal climatology, with thick layers and high abundance in winter but thin layers and low abundance near summer. Tidal amplitudes of Na density are large in February-April and August-November with a dominant peak between 85 and 90 km; they are weak in summer months (May-July). The Na density tidal phase profiles, while showing downward progression, show a significant and abrupt phase shift (ideally 180 degrees). The center altitude of this phase shifting (termed switching altitude) is found to coincide with the fractional tidal amplitude (tidal amplitude over diurnal mean) minimum about 2-4 km above the centroid altitude of the associated Na layer. Taking advantage of the established temperature tidal climatology deduced from the same data set, the tidal phase behaviors between temperature and Na density and associated fractional Na density tidal amplitudes are discussed in terms of the theoretical prediction by Gardner and Shelton (1985).

Yuan, Tao; She, C.-Y.; Kawahara, Takuya D.; Krueger, D. A.

2012-06-01

428

Diffusion and the dynamics of displacive phase transitions in cryolite (Na 3 AlF 6 ) and chiolite (Na 5 Al 3 F 14 ): Multinuclear NMR studies  

Microsoft Academic Search

Cryolite is a mixed-cation perovskite (Na2(NaAl)F6) which undergoes a monoclinic to orthorhombic displacive phase transition at ~550° C. Chiolite (Na5Al3F14) is associated with cryolite in natural deposits, and consists of sheets of corner sharing [AlF6] octahedra interlayered with edge-sharing [NaF6] octahedra. Multi-nuclear NMR line shape and relaxation time (T1) studies were performed on cryolite and chiolite in order to gain

Dane R. Spearing; Jonathan F. Stebbins; Ian Farnan

1994-01-01

429

Reactivity of NASICON with water and interpretation of the detection limit of a NASICON based Na + ion selective electrode  

Microsoft Academic Search

The leaching of Na+ ions from NASICON of composition Na3Zr2Si2PO12 to the aqueous solution was evidenced. The origin of the Na+ leaching was studied using Na+ concentration and pH measurements as well as solution and X-ray analyses. The Na+ released was mainly attributed to the dissolution of a second phase, predominantly amorphous. The rate of Na+ release was found to

F. Mauvy; E. Siebert; P. Fabry

1999-01-01

430

Modeling the sorption dynamics of NaH using a reactive force field  

SciTech Connect

We have parametrized a reactive force field for NaH, ReaxFF{sub NaH}, against a training set of ab initio derived data. To ascertain that ReaxFF{sub NaH} is properly parametrized, a comparison between ab initio heats of formation of small representative NaH clusters with ReaxFF{sub NaH} was done. The results and trend of ReaxFF{sub NaH} are found to be consistent with ab initio values. Further validation includes comparing the equations of state of condensed phases of Na and NaH as calculated from ab initio and ReaxFF{sub NaH}. There is a good match between the two results, showing that ReaxFF{sub NaH} is correctly parametrized by the ab initio training set. ReaxFF{sub NaH} has been used to study the dynamics of hydrogen desorption in NaH particles. We find that ReaxFF{sub NaH} properly describes the surface molecular hydrogen charge transfer during the abstraction process. Results on heat of desorption versus cluster size shows that there is a strong dependence on the heat of desorption on the particle size, which implies that nanostructuring enhances desorption process. To gain more insight into the structural transformations of NaH during thermal decomposition, we performed a heating run in a molecular dynamics simulation. These runs exhibit a series of drops in potential energy, associated with cluster fragmentation and desorption of molecular hydrogen. This is consistent with experimental evidence that NaH dissociates at its melting point into smaller fragments.

Ojwang, J. G. O.; Santen, Rutger van; Kramer, Gert Jan [Schuit Institute of Catalysis, Eindhoven University of Technology, Postbus 513, 5600 MB, Den Dolech 2, Eindhoven (Netherlands); Duin, Adri C. T. van; Goddard, William A. III [Material Research Center, California Institute of Technology (Caltech), 1200 East California Boulevard, Pasadena, California 91125 (United States)

2008-04-28

431

Search for solid conductors of Na/+/ and K/+/ ions - Five new conductors  

NASA Technical Reports Server (NTRS)

Five new conductors of positive Na and K ions, for use as separators in high energy secondary batteries, have been discovered. They include: (1) the pyrochlores NaTaWO6 and NaTa2O5F; (2) the bcc form of NaSbO3; and (3) the niobates 2Na2O-3Nb2O5 and 2K2O-3Nb2O5, with the alkali ions probably in open layers of the completely determined structure. On the basis of approximately 40 structure types, generalizations have been made regarding the relation between structure and ionic transport.

Singer, J.; Kautz, H.; Fielder, W.; Fordyce, J.

1975-01-01

432

In vitro, ethanol (ETOH) stimulates or inhibits Na/sup +/, K/sup +/-ATPase depending on Na/sup +//K/sup +/ ratios  

SciTech Connect

Effects of pharmacologically relevant levels of ETOH on porcine brain enzyme (PBE) and rat brain enzyme (RBE) were investigated. While varying Na/sup +/ and K/sup +/ levels, yet keeping their sum at 150mM, ETOH at 500mg/dL stimulated PBE when K/sup +/ > Na/sup +/ but inhibited when Na/sup +/ > K/sup +/. When Na/sup +//K/sup +/ = 15/90mM (intracellular conc.), ETOH stimulated PBE 48% but when Na/sup +//K/sup +/ = 144/6mM (extracellular conc.) ETOH inhibited 45%. For RBE, ETOH at 100, 300, and 450mg/dL stimulated 4%, 33%, and 20%, respectively, when Na/sup +//K/sup +/ = 15/88mM (intracellular) but inhibited 28%, 28%, and 25%, respectively, when Na/sup +//K/sup +/ = 142/4mM (extracellular). When Na/sup +/ is rate limiting, thus controlling enzyme activity from its high affinity site, ETOH is stimulatory but when K/sup +/ is rate limiting (controlling from its high affinity site) ETOH is inhibitory. These data could be explained by a tandem model for Na/sup +/ and K/sup +/ activation where ETOH promotes a shift from an intracellular to an extracellular facing conformation. Moreover, these data also strongly indicate that intoxicating levels of ETOH may affect the Na/sup +/, K/sup +/ -pump in vivo, especially in neurons which experience varying Na/sup +/ and K/sup +/ levels on each side of the cell membrane.

Foster, D.M.; Klemm, W.R.

1986-05-01

433

Temperature-Dependent Solubility Transition of Na2SO4 in Water and the Effect of NaCl Therein: Solution Structures and Salt Water Dynamics.  

PubMed

Dual, aqueous solubility behavior of Na2SO4 as a function of temperatures is still a natural enigma lying unresolved in the literature. The solubility of Na2SO4 increases up to 32.38 °C and decreases slightly thereafter at higher temperatures. We have thrown light on this phenomenon by analyzing the Na2SO4-water clusters (growth and stability) detected from temperature-dependent dynamic light scattering experiments, solution compressibility changes derived from the density and speed of sound measurements, and water structural changes/Na2SO4 (ion pair)-water interactions observed from the FT-IR and 2D DOSY (1)H NMR spectroscopic investigations. It has been observed that Na2SO4-water clusters grow with an increase in Na2SO4 concentration (until the solubility transition temperature) and then start decreasing afterward. An unusual decrease in cluster size and solution compressibility has been observed with the rise in temperature for the Na2SO4 saturated solutions below the solubility transition temperature, whereas an inverse pattern is followed thereafter. DOSY experiments have indicated different types of water cluster species in saturated solutions at different temperatures with varying self-diffusion coefficients. The effect of NaCl (5-15 wt %) on the solubility behavior of Na2SO4 at different temperatures has also been examined. The studies are important from both fundamental and industrial application points of view, for example, toward the clean separation of NaCl and Na2SO4 from the effluent streams of textile and tannery industries. PMID:25313635

Bharmoria, Pankaj; Gehlot, Praveen Singh; Gupta, Hariom; Kumar, Arvind

2014-11-01

434

Inhibition and activation of Na/sup +/-Ca/sup 2 +/ exchange activity by quinacrine  

SciTech Connect

Quinacrine either inhibited or stimulated Na-Ca exchange in cardiac sarcolemmal vesicles, depending on the experimental conditions. When present in the assay medium for Na-Ca exchange, quinacrine inhibited both Na/sub i/-dependent Ca/sup 2 +/ uptake (K/sub i/ = 50 ..mu..M) and Na/sub 0/-dependent Ca/sup 2 +/ efflux. Quinacrine's inhibition of Ca/sub 2//sup +/ efflux was attenuated by high concentrations of Na/sup +/. Quinacrine also blocked Na-Na and Ca-Ca exchange activities in the vesicles. The inhibitory effects of quinacrine on Na-Ca exchange activity are qualitatively similar to those reported previously for amiloride derivatives. When Na-loaded vesicles were preincubated with quinacrine and then assayed for Na-Ca exchange in a quinacrine-free medium, stimulation of exchange activity was observed. This stimulation was reversible on the removal of bound quinacrine and involved a reduction in the apparent K/sub m/ for extravesicular Ca/sup 2 +/. Stimulation of exchange activity under these conditions was also observed with the lipophilic cations tetraphenylphosphonium. Since Ca/sup 2 +/, quinacrine and tetraphenylphosphonium all bind strongly to sarcolemmal membranes it is suggested that the observed stimulation of exchange activity involves a local electrostatic effect of the bound cations in accelerating a rate-limiting step in the reaction mechanism for Na-Ca exchange.

de la Pena, P.; Reeves, J.P.

1987-01-01

435

Reversible stimulation of the Na+/K+ pump by monensin in cultures of vascular smooth muscle.  

PubMed

Two ionophores, monensin and salinomycin, increased total cell Na+ and ouabain-sensitive 86Rb+ uptake in cultures of smooth muscle cells from rat aorta. Monensin was used to produce graded increases in cell Na+ in order to assess the Na+ dependence of the Na+/K+ pump in the intact cell. The relationship between internal Na+ and ouabain-sensitive 86Rb+ uptake was hyperbolic (K'Na = 3 mM). Monensin did not stimulate 86Rb+ uptake in the absence of external Na+. Loading the cells with Na+ by exposing cultures to a K+-free medium for 3 hr maximally increased cell Na+ and ouabain-sensitive 86Rb+ uptake to the same extent as monensin. Total cell Na+ and pump activity in monensin-treated cells returned to the initial values after removing the ionophore. Monensin was then able to increase total cell Na+ and ouabain-sensitive 86Rb+ uptake to the same extent as the initial treatment with the ionophore. PMID:7132555

Brock, T A; Smith, J B

1982-09-01

436

Composite PEOn:NaTFSI polymer electrolyte: Preparation, thermal and electrochemical characterization  

NASA Astrophysics Data System (ADS)

Membranes of sodium bis(trifluoromethanesulfonate) imide (NaTFSI) complexed with poly(ethylene oxide) (PEO) salt have been prepared by a solvent-free hot-pressing technique with different EO:Na molar ratio. All membranes show good ionic conductivities in the range of 10-3 S cm-1 above 70 °C. However, the more NaTFSI-concentrated samples are sticky gums due to the plasticizing nature of the anion. The PEO20:NaTFSI sample exhibits the compromise of conductivity, thermal and mechanical properties. The addition of nanometric SiO2 to the PEO20:NaTFSI membranes further enhances their mechanical properties. Moreover, the PEO20:NaTFSI + 5 wt.% SiO2 membranes show similar ionic conductivity and similar anodic electrochemical stability in comparison to the ceramic free PEO20:NaTFSI sample. In a Na(s)/polymer electrolyte/Na(s) symmetrical cell followed up to 30 days, the presence of the ceramic filler slightly increased the interface resistance in comparison to the ceramic-free membrane. Nuclear magnetic resonance determinations of anion diffusion coefficients and Na+ mobility suggest that presence of filler may have a positive affect on the cation transference number that is in accordance with the tNa+ transference number measurement.

Serra Moreno, J.; Armand, M.; Berman, M. B.; Greenbaum, S. G.; Scrosati, B.; Panero, S.

2014-02-01

437

Salicylic acid alleviates NaCl-induced changes in the metabolism of Matricaria chamomilla plants.  

PubMed

Influence of 100 mM NaCl and 50 microM salicylic acid (SA) and their combination on the metabolism of chamomile (Matricaria chamomilla) during 7 days was studied. NaCl reduced growth and selected physiological parameters and SA in combined treatment (NaCl + SA) reversed majority of these symptoms. Application of SA reduced NaCl-induced increase of Na+ in the rosettes, but not in the roots. Accumulation of total amino acids was stimulated in NaCl-treated roots, especially due to exceptional increase of proline (4.4-fold). Among phenolic acids, accumulation of protocatechuic acid was the most enhanced in NaCl-exposed leaf rosettes (ca. 3-fold) while chlorogenic and caffeic acids in the roots (2.4- and 2.8-fold, respectively). Total soluble phenols increased after NaCl and SA treatments, but root lignin content was not affected. Activity of phenylalanine ammonia-lyase and shikimate dehydrogenase increased in response to NaCl, but cinnamyl alcohol dehydrogenase was not affected and polyphenol oxidase decreased. Stress parameters were elevated by NaCl treatment (superoxide radical and malondialdehyde content, activities of catalase, ascorbate- and guaiacol-peroxidase) and substantially prevented by SA, while accumulation of hydrogen peroxide decreased. Overall, SA showed strong beneficial properties against NaCl-induced negative symptoms. Protective effect of SA was the most visible at the level of guaiacol-peroxidase and through amelioration of stress parameters and mineral nutrient contents. PMID:19381803

Kovácik, Jozef; Klejdus, Borivoj; Hedbavny, Josef; Backor, Martin

2009-07-01

438

Ca/Na montmorillonite: structure, forces and swelling properties.  

PubMed

Ca/Na montmorillonite and natural Wyoming bentonite (MX-80) have been studied experimentally and theoretically. For a clay system in equilibrium with pure water, Monte Carlo simulations predict a large swelling when the clay counterions are monovalent, while in presence of divalent counterions a limited swelling is obtained with an aqueous layer between the clay platelets of about 10 A. This latter result is in excellent agreement with X-ray scattering data, while dialysis experiments give a significantly larger swelling for Ca montmorillonite in pure water. Obviously, there is one "intra-lamellar" and a second "extra-lamellar" swelling. Montmorillonite in contact with a salt reservoir containing both Na(+) and Ca(2+) counterions will only show a modest swelling unless the Na(+) concentration in the bulk is several orders of magnitude larger than the Ca(2+) concentration. The limited swelling of clay in presence of divalent counterions is a consequence of ion-ion correlations, which reduce the entropic repulsion as well as give rise to an attractive component in the total osmotic pressure. Ion-ion correlations also favor divalent counterions in a situation with a competition with monovalent ones. A more fundamental result of ion-ion correlations is that the osmotic pressure as a function of clay sheet separation becomes nonmonotonic, which indicates the possibility of a phase separation into a concentrated and a dilute clay phase, which would correspond to the "extra-lamellar" swelling found in dialysis experiments. This idea also finds support in the X-ray scattering spectra, where sometimes two peaks corresponding to different lamellar spacings appear. PMID:20235552

Segad, M; Jönsson, Bo; Akesson, T; Cabane, B

2010-04-20

439

Excimer lasers for superhigh NA 193-nm lithography  

NASA Astrophysics Data System (ADS)

Excimer lasers are widely used as the light source for microlithography scanners. The volume shipment of scanner systems using 193nm is projected to begin in year 2003. Such tools will directly start with super high numerical aperture (NA) in order to take full advantage of the 193nm wavelength over the advanced 248nm systems. Reliable high repetition rate laser light sources enabling high illumination power and wafer throughput are one of the fundamental prerequisites. In addition these light sources must support a very high NA imaging lens of more than 0.8 which determines the output spectrum of the laser to be less than 0.30 pm FWHM. In this paper we report on our recent progress in the development of high repetition rate ultra-narrow band lasers for high NA 193nm microlithography scanners. The laser, NovaLine A4003, is based on a Single Oscillator Ultral Line-narrowed (SOUL) design which yields a bandwidth of less than 0.30pm FWHM. The SOUL laser enables superior optical performance without adding complexity or cost up to the 4 kHz maximum repetition rate. The A4003's high precision line-narrowing optics used in combination with the high repetition rate of 4 kHz yields an output power of 20 W at an extremely narrow spectral bandwidth of less than 0.30 pm FWHM and highest spectral purity of less than 0.75 pm for the 95% energy content. We present performance and reliability data and discuss the key laser parameters. Improvements in the laser-internal metrology and faster regulation control result in better energy stability and improved overall operation behavior. The design considerations for line narrowing and stable laser operation at high repetition rates are discussed.

Paetzel, Rainer; Albrecht, Hans S.; Lokai, Peter; Zschocke, Wolfgang; Schmidt, Thomas; Bragin, Igor; Schroeder, Thomas; Reusch, Christian; Spratte, Stefan

2003-06-01

440

Andrade's Milagre na Cela: Theatrical Space and Body Movement  

E-print Network

, Milagre na Cela (1978), presents a decisive shift in both theme and structure. In a work about the torture of a prisoner of con science in a setting which is at the same time both highly contemporary and imbued with mythical resonance, the traditional... coherent present. In Marta, a Arvore e o Relógio, a collection of ten plays written between 1951 and 1970, he develops several recurrent themes: the search for identity in the aftermath of the fall of the fazendeiro economy, the effects upon the human...

Unruh, Vicky

1981-10-01

441

Project ELaNa and NASA's CubeSat Initiative  

NASA Technical Reports Server (NTRS)

This slide presentation reviews the NASA program to use expendable lift vehicles (ELVs) to launch nanosatellites for the purpose of enhancing educational research. The Education Launch of Nanosatellite (ELaNa) project, run out of the Launch Services Program is requesting proposals for CubeSat type payload to provide information that will aid or verify NASA Projects designs while providing opportunities for higher educational research. Some of the challenges involved with the program are discussed, and there is brief discussion about requirements for participation in the program, and the number of flight opportunities available for the launch of the Poly Picosatellite Orbital Deployer (PPOD).

Skrobot, Garrett Lee

2010-01-01

442

Polarizabilities of Li and Na in Debye plasmas  

SciTech Connect

We have carried out calculations to investigate the effect of Debye plasmas on the dipole, quadrupole, octupole polarizabilities of lithium and sodium atoms using the symplectic algorithm in the framework of the pseudo-state summation technique. The polarizabilities of alkali-metal atoms for various Debye lengths are reported for the first time in the literature. The behavior of the transition energies and oscillator strengths for Li and Na in plasma environments is also presented. In free atomic cases, our calculated results are in good agreement with the reported theoretical and experimental results.

Li, H. W. [Center for Theoretical Atomic and Molecular Physics, Academy of Fundamental and Interdisciplinary Sciences, Harbin Institute of Technology, Harbin 150080 (China); National Key Laboratory of Tunable Laser Technology, Harbin Institute of Technology, Harbin 150080 (China); Kar, Sabyasachi [Center for Theoretical Atomic and Molecular Physics, Academy of Fundamental and Interdisciplinary Sciences, Harbin Institute of Technology, Harbin 150080 (China)

2012-07-15

443

Analysis of Cl and Na in Hyperimmune Sera by NAA  

NASA Astrophysics Data System (ADS)

The Cl and Na concentration values in four types of hyperimmune sera (anti-Bothrops, anti-Diphtheria, anti-Rabies and anti-Tetanus) used for immunological therapy were determined using Neutron Activation Analysis (NAA). These data were compatible with the specifications established by the Word Health Organization (WHO-OMS) and with the Brazilian Official Pharmacopea (Pharmaceutical Code Official of the Country). These data are an important support for quality control of hyperimmune sera production at Butantan Institute (São Paulo city, Brazil), responsible for supplying the Brazilian market.

Baptista, T. S.; Zamboni, C. B.; Marcelino, J. R.

2011-08-01

444

Characterization of NaCl tolerance in Desulfovibrio vulgaris Hildenborough through experimental evolution  

PubMed Central

Desulfovibrio vulgaris Hildenborough strains with significantly increased tolerance to NaCl were obtained via experimental evolution. A NaCl-evolved strain, ES9-11, isolated from a population cultured for 1200 generations in medium amended with 100?mM NaCl, showed better tolerance to NaCl than a control strain, EC3-10, cultured for 1200 generations in parallel but without NaCl amendment in medium. To understand the NaCl adaptation mechanism in ES9-11, we analyzed the transcriptional, metabolite and phospholipid fatty acid (PLFA) profiles of strain ES9-11 with 0, 100- or 250?mM-added NaCl in medium compared with the ancestral strain and EC3-10 as controls. In all the culture conditions, increased expressions of genes involved in amino-acid synthesis and transport, energy production, cation efflux and decreased expression of flagellar assembly genes were detected in ES9-11. Consistently, increased abundances of organic solutes and decreased cell motility were observed in ES9-11. Glutamate appears to be the most important osmoprotectant in D. vulgaris under NaCl stress, whereas, other organic solutes such as glutamine, glycine and glycine betaine might contribute to NaCl tolerance under low NaCl concentration only. Unsaturation indices of PLFA significantly increased in ES9-11. Branched unsaturated PLFAs i17:1 ?9c, a17:1 ?9c and branched saturated i15:0 might have important roles in maintaining proper membrane fluidity under NaCl stress. Taken together, these data suggest that the accumulation of osmolytes, increased membrane fluidity, decreased cell motility and possibly an increased exclusion of Na+ contribute to increased NaCl tolerance in NaCl-evolved D. vulgaris. PMID:23575373

Zhou, Aifen; Baidoo, Edward; He, Zhili; Mukhopadhyay, Aindrila; Baumohl, Jason K; Benke, Peter; Joachimiak, Marcin P; Xie, Ming; Song, Rong; Arkin, Adam P; Hazen, Terry C; Keasling, Jay D; Wall, Judy D; Stahl, David A; Zhou, Jizhong

2013-01-01

445

Na,K-ATPase activity in mouse muscle is regulated by AMPK and PGC-1?.  

PubMed

Na,K-ATPase activity, which is crucial for skeletal muscle function, undergoes acute and long-term regulation in response to muscle activity. The aim of the present study was to test the hypothesis that AMP kinase (AMPK) and the transcriptional coactivator PGC-1? are underlying factors in long-term regulation of Na,K-ATPase isoform (?,? and PLM) abundance and Na(+) affinity. Repeated treatment of mice with the AMPK activator AICAR decreased total PLM protein content but increased PLM phosphorylation, whereas the number of ?- and ?-subunits remained unchanged. The K(m) for Na(+) stimulation of Na,K-ATPase was reduced (higher affinity) after AICAR treatment. PLM abundance was increased in AMPK kinase-dead mice compared with control mice, but PLM phosphorylation and Na,K-ATPase Na(+) affinity remained unchanged. Na,K-ATPase activity and subunit distribution were also measured in mice with different degrees of PGC-1? expression. Protein abundances of ?1 and ?2 were reduced in PGC-1? +/- and -/- mice, and the ?(1)/?(2) ratio was increased with PGC-1? overexpression (TG mice). PLM protein abundance was decreased in TG mice, but phosphorylation status was unchanged. Na,K-ATPase V (max) was decreased in PCG-1? TG and KO mice. Experimentally in vitro induced phosphorylation of PLM increased Na,K-ATPase Na(+) affinity, confirming that PLM phosphorylation is important for Na,K-ATPase function. In conclusion, both AMPK and PGC-1? regulate PLM abundance, AMPK regulates PLM phosphorylation and PGC-1? expression influences Na,K-ATPase ?(1) and ?(2) content and ?(1)/?(2) isoform ratio. Phosphorylation of the Na,K-ATPase subunit PLM is an important regulatory mechanism. PMID:21687978

Ingwersen, Maria S; Kristensen, Michael; Pilegaard, Henriette; Wojtaszewski, Jørgen F P; Richter, Erik A; Juel, Carsten

2011-07-01

446

An in vitro investigation of gastrointestinal Na(+) uptake mechanisms in freshwater rainbow trout.  

PubMed

In vitro gut-sac preparations of all four sections (stomach, anterior, mid, and posterior intestine) of the gastrointestinal tract (GIT) of freshwater rainbow trout, together with radiotracer ((22)Na) techniques, were used to study unidirectional Na(+) uptake rates (UR, mucosal ? blood space) and net absorptive fluid transport rates (FTR) under isosmotic conditions (mucosal = serosal osmolality). On an area-specific basis, unidirectional Na(+) UR was highest in the mid-intestine, but when total gut area was taken into account, the three intestinal sections contributed equally, with very low rates in the stomach. The theoretical capacity for Na(+) uptake across the whole GIT is sufficient to supply all of the animal's nutritive requirements for Na(+). Transport occurs by low affinity systems with apparent K m values 2-3 orders of magnitude higher than those in the gills, in accord with comparably higher Na(+) concentrations in chyme versus fresh water. Fluid transport appeared to be Na(+)-dependent, such that treatments which altered unidirectional Na(+) UR generally altered FTR in a comparable fashion. Pharmacological trials (amiloride, EIPA, phenamil, bafilomycin, furosemide, hydrochlorothiazide) conducted at a mucosal Na(+) concentration of 50 mmol L(-1) indicated that GIT Na(+) uptake occurs by a variety of apical mechanisms (NHE, Na(+) channel/H(+) ATPase, NCC, NKCC) with relative contributions varying among sections. However, at a mucosal Na(+) concentration of 10 mmol L(-1), EIPA, phenamil, bafilomycin, and hydrochlorothiazide were no longer effective in inhibiting unidirectional Na(+) UR or FTR, suggesting the contribution of unidentified mechanisms under low Na(+) conditions. A preliminary model is presented. PMID:25183198

Nadella, Sunita R; Patel, Dhanisha; Ng, Allen; Wood, Chris M

2014-12-01

447

Hydrothermal crystallization of Na2Ti6O13, Na2Ti3O7, and Na16Ti10O28 in the NaOH-TiO2-H2O system at a temperature of 500°C and a pressure of 0.1 GPa: The structural mechanism of self-assembly of titanates from suprapolyhedral clusters  

NASA Astrophysics Data System (ADS)

An increase in the NaOH concentration in the NaOH-TiO2 (rutile)-H2O system at a temperature of 500°C and a pressure of 0.1 GPa leads to the crystallization R-TiO2 + Na2Ti6O13 ? Na2Ti3O7 ? Na16Ti10O28. Crystals of the Na2Ti6O13 titanate (space group C2/m) have the three-dimensional framework structure Ti6O13. The structure of the Na2Ti3O7 titanate (space group P2 1/ m) contains the two-dimensional layers Ti3O7. The structure of the Na16Ti10O28 titanate (space group P-1) is composed of the isolated ten-polyhedron cluster precursors Ti10O28. In all the structures, the titanium atoms have an octahedral coordination ( MTiO6). The matrix self-assembly of the Na2Ti6O13 and Na2Ti3O7 (Na4Ti6O14) crystal structures from Na4 M 12 invariant precursors is modeled. These precursors are clusters consisting of twelve M polyhedra linked through the edges. It is demonstrated that the structurally rigid precursors Na4 M 12 control all processes of the subsequent evolution of the crystal-forming titanate clusters. The specific features of the self-assembly of the Na2Ti3O7 structure that result from the additional incorporation of twice the number of sodium atoms into the composition of the high-level clusters are considered.

Hyushin, G. D.

2006-07-01

448

The inhibitory effect of hormones associated with stress on Na appetite of sheep.  

PubMed

Stress is a large stimulus of Na appetite in rabbits, rats, and mice. This study investigated the influence of some peptides implicated in stress, i.e., adrenocorticotropin (ACTH), corticotropin-releasing factor (CRF), and the recently discovered member of the CRF family, urocortin, on the ingestive behavior of sheep. Intracerebroventricular infusion of these peptides over 4 days decreased the need-free Na intake of Na-repleted sheep. Intracerebroventricular infusion of urocortin, however, did not alter Na intake of Na-depleted sheep. Systemic infusion of ACTH increased, whereas systemic infusion of either urocortin or CRF decreased, Na intake of Na-repleted sheep. The increase in Na intake caused by the peripheral infusion of ACTH was blocked by concurrent i.v. infusion of urocortin, substantiating the inhibitory role of this peptide on Na appetite. Central administration of all peptides and i.v. administration of urocortin or urocortin and ACTH combined decreased food intake. Water intake was not directly influenced by the peptides. Rather, decreased water intake, when observed, was secondary to decreased food intake, as determined by pair-feeding experiments. Whereas systemic infusion of ACTH mimics the increase in Na intake observed in several different stressful situations, CRF and urocortin actually inhibit Na intake, indicating a direct central action overriding any effect of these peptides on ACTH release. Indeed, the inhibition of Na intake by urocortin occurred despite its stimulation of ACTH release and the subsequent increase in peripheral level of cortisol. Thus it would appear that hormones associated with stress have both excitatory and inhibitory influences on Na intake. Presumably, other physiological processes entrained by stress also will be important in determining the quantitative outcome on Na appetite. PMID:10717005

Weisinger, R S; Blair-West, J R; Burns, P; Denton, D A; McKinley, M J; Purcell, B; Vale, W; Rivier, J; Sunagawa, K

2000-03-14

449

Na2Ni2TeO6: Evaluation as a cathode for sodium battery  

NASA Astrophysics Data System (ADS)

The sodium layered oxides Na2Ni2TeO6, Na2Co2TeO6 and Na1.6Sr0.2Ni2TeO6 have been synthesized by conventional solid-state method. The samples were characterized by X-ray diffractometry (XRD), cyclic voltammetry (CV), scanning electron microscopy (SEM) and charge-discharge measurements. The results show that all the samples crystallize in a hexagonal unit cell without impurity. The electrochemical Na+ extraction (i.e., the charge profile) for Na2Ni2TeO6 exhibits two voltage plateaus at 3.6 and 4.4 V, the first corresponds to Na+ ordering at extraction of 1/3 of the sodium ions per formula unit, the second to the onset of SEI formation. Detailed XPS analysis confirms that the Ni(III) does not disproportionate into Ni(III) and Ni(IV). The process of Na extraction/insertion in the interlayers of Na2Ni2TeO6 is highly reversible with a capacity of 100 mAh g-1 at slow cycling rate (0.03 C) in the 4.35 to 3 V voltage range versus Na+/Na. The lattice constant and the unit-cell volume increase with the substitution of Na+ by Sr2+. In the presence of Sr2+, the appearance of a step in the charge-discharge profile of Na1.6Sr0.2Ni2TeO6 is suppressed. Na2Co2TeO6 showed little Na extraction/insertion.

Gupta, Asha; Buddie Mullins, C.; Goodenough, John B.

2013-12-01

450

The gasotransmitter hydrogen sulphide decreases Na? transport across pulmonary epithelial cells.  

PubMed

BACKGROUND AND PURPOSE The transepithelial absorption of Na(+) in the lungs is crucial for the maintenance of the volume and composition of epithelial lining fluid. The regulation of Na(+) transport is essential, because hypo- or hyperabsorption of Na(+) is associated with lung diseases such as pulmonary oedema or cystic fibrosis. This study investigated the effects of the gaseous signalling molecule hydrogen sulphide (H(2) S) on Na(+) absorption across pulmonary epithelial cells. EXPERIMENTAL APPROACH Ion transport processes were electrophysiologically assessed in Ussing chambers on H441 cells grown on permeable supports at air/liquid interface and on native tracheal preparations of pigs and mice. The effects of H(2)S were further investigated on Na(+) channels expressed in Xenopus oocytes and Na(+) /K(+)-ATPase activity in vitro. Membrane abundance of Na(+) /K(+)-ATPase was determined by surface biotinylation and Western blot. Cellular ATP concentrations were measured colorimetrically, and cytosolic Ca(2+) concentrations were measured with Fura-2. KEY RESULTS H(2)S rapidly and reversibly inhibited Na(+) transport in all the models employed. H(2)S had no effect on Na(+) channels, whereas it decreased Na(+) /K(+)-ATPase currents. H(2)S did not affect the membrane abundance of Na(+) /K(+)-ATPase, its metabolic or calcium-dependent regulation, or its direct activity. However, H(2)S inhibited basolateral calcium-dependent K(+) channels, which consequently decreased Na(+) absorption by H441 monolayers. CONCLUSIONS AND IMPLICATIONS H(2) S impairs pulmonary transepithelial Na(+) absorption, mainly by inhibiting basolateral Ca(2+)-dependent K(+) channels. These data suggest that the H(2)S signalling system might represent a novel pharmacological target for modifying pulmonary transepithelial Na(+) transport. PMID:22352810

Althaus, M; Urness, K D; Clauss, W G; Baines, D L; Fronius, M

2012-07-01

451

The gasotransmitter hydrogen sulphide decreases Na+ transport across pulmonary epithelial cells  

PubMed Central

BACKGROUND AND PURPOSE The transepithelial absorption of Na+ in the lungs is crucial for the maintenance of the volume and composition of epithelial lining fluid. The regulation of Na+ transport is essential, because hypo- or hyperabsorption of Na+ is associated with lung diseases such as pulmonary oedema or cystic fibrosis. This study investigated the effects of the gaseous signalling molecule hydrogen sulphide (H2S) on Na+ absorption across pulmonary epithelial cells. EXPERIMENTAL APPROACH Ion transport processes were electrophysiologically assessed in Ussing chambers on H441 cells grown on permeable supports at air/liquid interface and on native tracheal preparations of pigs and mice. The effects of H2S were further investigated on Na+ channels expressed in Xenopus oocytes and Na+/K+-ATPase activity in vitro. Membrane abundance of Na+/K+-ATPase was determined by surface biotinylation and Western blot. Cellular ATP concentrations were measured colorimetrically, and cytosolic Ca2+ concentrations were measured with Fura-2. KEY RESULTS H2S rapidly and reversibly inhibited Na+ transport in all the models employed. H2S had no effect on Na+ channels, whereas it decreased Na+/K+-ATPase currents. H2S did not affect the membrane abundance of Na+/K+-ATPase, its metabolic or calcium-dependent regulation, or its direct activity. However, H2S inhibited basolateral calcium-dependent K+ channels, which consequently decreased Na+ absorption by H441 monolayers. CONCLUSIONS AND IMPLICATIONS H2S impairs pulmonary transepithelial Na+ absorption, mainly by inhibiting basolateral Ca2+-dependent K+ channels. These data suggest that the H2S signalling system might represent a novel pharmacological target for modifying pulmonary transepithelial Na+ transport. PMID:22352810

Althaus, M; Urness, KD; Clauss, WG; Baines, DL; Fronius, M

2012-01-01

452

Quantification of Na+,K+ pumps and their transport rate in skeletal muscle: functional significance.  

PubMed

During excitation, muscle cells gain Na(+) and lose K(+), leading to a rise in extracellular K(+) ([K(+)]o), depolarization, and loss of excitability. Recent studies support the idea that these events are important causes of muscle fatigue and that full use of the Na(+),K(+)-ATPase (also known as the Na(+),K(+) pump) is often essential for adequate clearance of extracellular K(+). As a result of their electrogenic action, Na(+),K(+) pumps also help reverse depolarization arising during excitation, hyperkalemia, and anoxia, or from cell damage resulting from exercise, rhabdomyolysis, or muscle diseases. The ability to evaluate Na(+),K(+)-pump function and the capacity of the Na(+),K(+) pumps to fill these needs require quantification of the total content of Na(+),K(+) pumps in skeletal muscle. Inhibition of Na(+),K(+)-pump activity, or a decrease in their content, reduces muscle contractility. Conversely, stimulation of the Na(+),K(+)-pump transport rate or increasing the content of Na(+),K(+) pumps enhances muscle excitability and contractility. Measurements of [(3)H]ouabain binding to skeletal muscle in vivo or in vitro have enabled the reproducible quantification of the total content of Na(+),K(+) pumps in molar units in various animal species, and in both healthy people and individuals with various diseases. In contrast, measurements of 3-O-methylfluorescein phosphatase activity associated with the Na(+),K(+)-ATPase may show inconsistent results. Measurements of Na(+) and K(+) fluxes in intact isolated muscles show that, after Na(+) loading or intense excitation, all the Na(+),K(+) pumps are functional, allowing calculation of the maximum Na(+),K(+)-pumping capacity, expressed in molar units/g muscle/min. The activity and content of Na(+),K(+) pumps are regulated by exercise, inactivity, K(+) deficiency, fasting, age, and several hormones and pharmaceuticals. Studies on the ?-subunit isoforms of the Na(+),K(+)-ATPase have detected a relative increase in their number in response to exercise and the glucocorticoid dexamethasone but have not involved their quantification in molar units. Determination of ATPase activity in homogenates and plasma membranes obtained from muscle has shown ouabain-suppressible stimulatory effects of Na(+) and K(+). PMID:24081980

Clausen, Torben

2013-10-01

453

Quantification of Na+,K+ pumps and their transport rate in skeletal muscle: Functional significance  

PubMed Central

During excitation, muscle cells gain Na+ and lose K+, leading to a rise in extracellular K+ ([K+]o), depolarization, and loss of excitability. Recent studies support the idea that these events are important causes of muscle fatigue and that full use of the Na+,K+-ATPase (also known as the Na+,K+ pump) is often essential for adequate clearance of extracellular K+. As a result of their electrogenic action, Na+,K+ pumps also help reverse depolarization arising during excitation, hyperkalemia, and anoxia, or from cell damage resulting from exercise, rhabdomyolysis, or muscle diseases. The ability to evaluate Na+,K+-pump function and the capacity of the Na+,K+ pumps to fill these needs require quantification of the total content of Na+,K+ pumps in skeletal muscle. Inhibition of Na+,K+-pump activity, or a decrease in their content, reduces muscle contractility. Conversely, stimulation of the Na+,K+-pump transport rate or increasing the content of Na+,K+ pumps enhances muscle excitability and contractility. Measurements of [3H]ouabain binding to skeletal muscle in vivo or in vitro have enabled the reproducible quantification of the total content of Na+,K+ pumps in molar units in various animal species, and in both healthy people and individuals with various diseases. In contrast, measurements of 3-O-methylfluorescein phosphatase activity associated with the Na+,K+-ATPase may show inconsistent results. Measurements of Na+ and K+ fluxes in intact isolated muscles show that, after Na+ loading or intense excitation, all the Na+,K+ pumps are functional, allowing calculation of the maximum Na+,K+-pumping capacity, expressed in molar units/g muscle/min. The activity and content of Na+,K+ pumps are regulated by exercise, inactivity, K+ deficiency, fasting, age, and several hormones and pharmaceuticals. Studies on the ?-subunit isoforms of the Na+,K+-ATPase have detected a relative increase in their number in response to exercise and the glucocorticoid dexamethasone but have not involved their quantification in molar units. Determination of ATPase activity in homogenates and plasma membranes obtained from muscle has shown ouabain-suppressible stimulatory effects of Na+ and K+. PMID:24081980

2013-01-01

454

Osmolality- and Na+ -dependent effects of hyperosmotic NaCl solution on contractile activity and Ca2+ cycling in rat ventricular myocytes.  

PubMed

Hypertonic NaCl solutions have been used for small-volume resuscitation from hypovolemic shock. We sought to identify osmolality- and Na(+)-dependent components of the effects of the hyperosmotic NaCl solution (85 mOsm/kg increment) on contraction and cytosolic Ca(2+) concentration ([Ca(2+)](i)) in isolated rat ventricular myocytes. The biphasic change in contraction and Ca(2+) transient amplitude (decrease followed by recovery) was accompanied by qualitatively similar changes in sarcoplasmic reticulum (SR) Ca(2+) content and fractional release and was mimicked by isosmotic, equimolar increase in extracellular [Na(+)] ([Na(+)](o)). Raising osmolality with sucrose, however, augmented systolic [Ca(2+)](i) monotonically without change in SR parameters and markedly decreased contraction amplitude and diastolic cell length. Functional SR inhibition with thapsigargin abolished hyperosmolality effects on [Ca(2+)](i). After 15-min perfusion, both hyperosmotic solutions slowed mechanical relaxation during twitches and [Ca(2+)](i) decline during caffeine-evoked transients, raised diastolic and systolic