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Sample records for jet mk ii

  1. Identification of the sites for CaMK-II-dependent phosphorylation of GABA(A) receptors.

    PubMed

    Houston, Catriona M; Lee, Henry H C; Hosie, Alastair M; Moss, Stephen J; Smart, Trevor G

    2007-06-15

    Phosphorylation can affect both the function and trafficking of GABA(A) receptors with significant consequences for neuronal excitability. Serine/threonine kinases can phosphorylate the intracellular loops between M3-4 of GABA(A) receptor beta and gamma subunits thereby modulating receptor function in heterologous expression systems and in neurons (1, 2). Specifically, CaMK-II has been demonstrated to phosphorylate the M3-4 loop of GABA(A) receptor subunits expressed as GST fusion proteins (3, 4). It also increases the amplitude of GABA(A) receptor-mediated currents in a number of neuronal cell types (5-7). To identify which substrate sites CaMK-II might phosphorylate and the consequent functional effects, we expressed recombinant GABA(A) receptors in NG108-15 cells, which have previously been shown to support CaMK-II modulation of GABA(A) receptors containing the beta3 subunit (8). We now demonstrate that CaMK-II mediates its effects on alpha1beta3 receptors via phosphorylation of Ser(383) within the M3-4 domain of the beta subunit. Ablation of beta3 subunit phosphorylation sites for CaMK-II revealed that for alphabetagamma receptors, CaMK-II has a residual effect on GABA currents that is not mediated by previously identified sites of CaMK-II phosphorylation. This residual effect is abolished by mutation of tyrosine phosphorylation sites, Tyr(365) and Tyr(367), on the gamma2S subunit, and by the tyrosine kinase inhibitor genistein. These results suggested that CaMK-II is capable of directly phosphorylating GABA(A) receptors and activating endogenous tyrosine kinases to phosphorylate the gamma2 subunit in NG108-15 cells. These findings were confirmed in a neuronal environment by expressing recombinant GABA(A) receptors in cerebellar granule neurons. PMID:17442679

  2. Differential expression of CaMK-II genes during early zebrafish embryogenesis.

    PubMed

    Rothschild, Sarah C; Lister, James A; Tombes, Robert M

    2007-01-01

    CaMK-II is a highly conserved Ca(2+)/calmodulin-dependent protein kinase expressed throughout the lifespan of all vertebrates. During early development, CaMK-II regulates cell cycle progression and "non-canonical" Wnt-dependent convergent extension. In the zebrafish, Danio rerio, CaMK-II activity rises within 2 hr after fertilization. At the time of somite formation, zygotic expression from six genes (camk2a1, camk2b1, camk2g1, camk2g2, camk2d1, camk2d2) results in a second phase of increased activity. Zebrafish CaMK-II genes are 92-95% identical to their human counterparts in the non-variable regions. During the first three days of development, alternative splicing yields at least 20 splice variants, many of which are unique. Whole-mount in situ hybridization reveals that camk2g1 comprises the majority of maternal expression. All six genes are expressed strongly in ventral regions at the 18-somite stage. Later, camk2a1 is expressed in anterior somites, heart, and then forebrain. Camk2b1 is expressed in somites, mid- and forebrain, gut, retina, and pectoral fins. Camk2g1 appears strongly along the midline and then in brain, gut, and pectoral fins. Camk2g2 is expressed early in the midbrain and trunk and exhibits the earliest retinal expression. Camk2d1 is elevated early at somite boundaries, then epidermal tissue, while camk2d2 is expressed in discrete anterior locations, steadily increasing along either side of the dorsal midline and then throughout the brain, including the retina. These findings reveal a complex pattern of CaMK-II gene expression consistent with pleiotropic roles during development. PMID:17103413

  3. Carbon-11 labeling of nonpeptide angiotensin-II antogonists: MK-996 and analogs

    SciTech Connect

    Mathews, W.B.; Burns, H.D.; Hamill, T.D.

    1994-05-01

    Our goal was to develop a tracer that can be used for imaging of angiotensin-II (ANG-II), AT{sub 1} receptors in vivo via PET. MK-996 is a potent, nonpeptide, benzamide-based, AT{sub 1} selective antagonist (IC{sub 50}=0.15 nM) in clinical trials for the treatment of hypertension. (C-11)MK-996 was prepared using (C-11)benzoyl chloride synthesized in two steps from (C-11) carbon dioxide via (C-11)benzoic acid. The labeled acid chloride was made by reacting the (C-11)benzoic acid with either phthaloyl dichloride or thionyl chloride. After HPLC purification, the (C-11)MK-996 with an average synthesis time of 38 minutes from EOB, a non-decay corrected radiochemical yield of 3%, and a specific activity of 1162 Ci/mmol at EOS. Although this route provided the radiotracer in sufficient quantities for imaging studies, the synthesis was cumbersome. Thus, a labeled tracer that could be prepared more conveniently was sought.

  4. Validating the Serpent Model of FiR 1 Triga Mk-II Reactor by Means of Reactor Dosimetry

    NASA Astrophysics Data System (ADS)

    Viitanen, Tuomas; Leppänen, Jaakko

    2016-02-01

    A model of the FiR 1 Triga Mk-II reactor has been previously generated for the Serpent Monte Carlo reactor physics and burnup calculation code. In the current article, this model is validated by comparing the predicted reaction rates of nickel and manganese at 9 different positions in the reactor to measurements. In addition, track-length estimators are implemented in Serpent 2.1.18 to increase its performance in dosimetry calculations. The usage of the track-length estimators is found to decrease the reaction rate calculation times by a factor of 7-8 compared to the standard estimator type in Serpent, the collision estimators. The differences in the reaction rates between the calculation and the measurement are below 20%.

  5. Jet Charge Tagging at CDF using Run II Data

    NASA Astrophysics Data System (ADS)

    Paus, Christoph

    2004-05-01

    We present a study of the jet charge tagging in Run II CDF. The jet charge tagging method is applied to determine the b/barb-quark flavor of B hadrons at the time of production in a sample of b arrow ℓ ν X decays collected in 2002-2003.

  6. Verification of MCNP simulation of neutron flux parameters at TRIGA MK II reactor of Malaysia.

    PubMed

    Yavar, A R; Khalafi, H; Kasesaz, Y; Sarmani, S; Yahaya, R; Wood, A K; Khoo, K S

    2012-10-01

    A 3-D model for 1 MW TRIGA Mark II research reactor was simulated. Neutron flux parameters were calculated using MCNP-4C code and were compared with experimental results obtained by k(0)-INAA and absolute method. The average values of φ(th),φ(epi), and φ(fast) by MCNP code were (2.19±0.03)×10(12) cm(-2)s(-1), (1.26±0.02)×10(11) cm(-2)s(-1) and (3.33±0.02)×10(10) cm(-2)s(-1), respectively. These average values were consistent with the experimental results obtained by k(0)-INAA. The findings show a good agreement between MCNP code results and experimental results. PMID:22885391

  7. Jet-intracluster medium interaction in Hydra A - II. The effect of jet precession

    NASA Astrophysics Data System (ADS)

    Nawaz, M. A.; Bicknell, G. V.; Wagner, A. Y.; Sutherland, R. S.; McNamara, B. R.

    2016-05-01

    We present three-dimensional relativistic hydrodynamical simulations of a precessing jet interacting with the intracluster medium and compare the simulated jet structure with the observed structure of the Hydra A northern jet. For the simulations, we use jet parameters obtained in the parameter space study of the first paper in this series and probe different values for the precession period and precession angle. We find that for a precession period P ≈ 1 Myr and a precession angle ψ ≈ 20°, the model reproduces (i) the curvature of the jet, (ii) the correct number of bright knots within 20 kpc at approximately correct locations and (iii) the turbulent transition of the jet to a plume. The Mach number of the advancing bow shock ≈1.85 is indicative of gentle cluster atmosphere heating during the early stages of the AGN's activity.

  8. HOT ELECTROMAGNETIC OUTFLOWS. II. JET BREAKOUT

    SciTech Connect

    Russo, Matthew; Thompson, Christopher

    2013-08-20

    We consider the interaction between radiation, matter, and a magnetic field in a compact, relativistic jet. The entrained matter accelerates outward as the jet breaks out of a star or other confining medium. In some circumstances, such as gamma-ray bursts (GRBs), the magnetization of the jet is greatly reduced by an advected radiation field while the jet is optically thick to scattering. Where magnetic flux surfaces diverge rapidly, a strong outward Lorentz force develops and radiation and matter begin to decouple. The increase in magnetization is coupled to a rapid growth in Lorentz factor. We take two approaches to this problem. The first examines the flow outside the fast magnetosonic critical surface, and calculates the flow speed and the angular distribution of the radiation field over a range of scattering depths. The second considers the flow structure on both sides of the critical surface in the optically thin regime, using a relaxation method. In both approaches, we find how the terminal Lorentz factor and radial profile of the outflow depend on the radiation intensity and optical depth at breakout. The effect of bulk Compton scattering on the radiation spectrum is calculated by a Monte Carlo method, while neglecting the effects of internal dissipation. The peak of the scattered spectrum sits near the seed peak if radiation pressure dominates the acceleration, but is pushed to a higher frequency if the Lorentz force dominates. The unscattered seed radiation can form a distinct, low-frequency component of the spectrum, especially if the magnetic Poynting flux dominates.

  9. BIPOLAR JETS LAUNCHED FROM ACCRETION DISKS. II. THE FORMATION OF ASYMMETRIC JETS AND COUNTER JETS

    SciTech Connect

    Fendt, Christian; Sheikhnezami, Somayeh E-mail: nezami@mpia.de

    2013-09-01

    We investigate the jet launching from accretion disks, in particular the formation of intrinsically asymmetric jet/counter jet systems. We perform axisymmetric MHD simulations of the disk-jet structure on a bipolar computational domain covering both hemispheres. We apply various models such as asymmetric disks with (initially) different scale heights in each hemisphere, symmetric disks into which a local disturbance is injected, and jets launched into an asymmetric disk corona. We consider both a standard global magnetic diffusivity distribution and a novel local diffusivity model. Typical disk evolution first shows substantial disk warping and then results in asymmetric outflows with a 10%-30% mass flux difference. We find that the magnetic diffusivity profile is essential for establishing a long-term outflow asymmetry. We conclude that bipolar asymmetry in protostellar and extragalactic jets can indeed be generated intrinsically and maintained over a long time by disk asymmetries and the standard jet launching mechanism.

  10. Turbulence measurements in axisymmetric jets of air and helium. I - Air jet. II - Helium jet

    NASA Astrophysics Data System (ADS)

    Panchapakesan, N. R.; Lumley, J. L.

    1993-01-01

    Results are presented of measurements on turbulent round jets of air and of helium of the same nozzle momentum efflux, using, for the air jets, x-wire hot-wire probes mounted on a moving shuttle and, for He jets, a composite probe consisting of an interference probe of the Way-Libby type and an x-probe. Current models for scalar triple moments were evaluated. It was found that the performance of the model termed the Full model, which includes all terms except advection, was very good for both the air and the He jets.

  11. MiR-152 May Silence Translation of CaMK II and Induce Spontaneous Immune Tolerance in Mouse Liver Transplantation

    PubMed Central

    Wang, Jingcheng; Yan, Sheng; Zhou, Lin; Xie, Haiyang; Chen, Hui; Li, Hui; Zhang, Jinhua; Zhao, Jiacong; Zheng, Shusen

    2014-01-01

    Spontaneous immune tolerance in mouse liver transplantation has always been a hotspot in transplantation-immune research. Recent studies revealed that regulatory T cells (Tregs), hepatic satellite cells and Kupffer cells play a potential role in spontaneous immune tolerance, however the precise mechanism of spontaneous immune tolerance is still undefined. By using Microarray Chips, we investigated different immune regulatory factors to decipher critical mechanisms of spontaneous tolerance after mouse liver transplantation. Allogeneic (C57BL/6-C3H) and syngeneic (C3H-C3H) liver transplantation were performed by 6-8 weeks old male C57BL/6 and C3H mice. Graft samples (N = 4 each group) were collected from 8 weeks post-operation mice. 11 differentially expressed miRNAs in allogeneic grafts (Allografts) vs. syngeneic grafts (Syngrafts) were identified using Agilent Mouse miRNA Chips. It was revealed that 185 genes were modified by the 11 miRNAs, furthermore, within the 185 target genes, 11 of them were tightly correlated with immune regulation after Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Genbank data cross-comparison. Verified by real-time PCR and western blot, our results indicated that mRNA expression levels of IL-6 and TAB2 were respectively down regulated following miR-142-3p and miR-155 augment. In addition, increased miR-152 just silenced mRNA of CaMK II and down-regulated translation of CaMK II in tolerated liver grafts, which may play a critical role in immune regulation and spontaneous tolerance induction of mouse liver transplantation. PMID:25133393

  12. A Protostellar Jet Emanating from a Hypercompact H ii Region

    NASA Astrophysics Data System (ADS)

    Guzmán, Andrés E.; Garay, Guido; Rodríguez, Luis F.; Contreras, Yanett; Dougados, Catherine; Cabrit, Sylvie

    2016-08-01

    We present radio continuum observations of the high-mass young stellar object (HMYSO) G345.4938+01.4677 obtained using the Australia Telescope Compact Array (ATCA) at 5, 9, 17, and 19 GHz. These observations provide definite evidence that the outer and inner pairs of radio lobes consist of shock-ionized material being excited by an underlying collimated and fast protostellar jet emanating from a hypercompact H ii region. By comparing with images taken 6 years earlier at 5 and 9 GHz using the same telescope, we assess the proper motions (PMs) of the radio sources. The outer west and east lobes exhibit PMs of 64 ± 12 and 48 ± 13 mas yr‑1, indicating velocities projected in the plane of the sky and receding from G345.4938+01.4677 of 520 and 390 {\\text{km s}}-1, respectively. The internal radio lobes also display PM signals consistently receding from the HMYSO with magnitudes of 17 ± 11 and 35 ± 10 mas yr‑1 for the inner west and east lobes, respectively. The morphology of the outer west lobe is that of a detached bow shock. At 17 and 19 GHz, the outer east lobe displays an arcuate morphology also suggesting a bow shock. These results show that disk accretion and jet acceleration—possibly occurring in a very similar way compared with low-mass protostars—is taking place in G345.4938+01.4677 despite the presence of ionizing radiation and the associated hypercompact H ii region.

  13. A large-volume high-pressure and high-temperature apparatus for in situ X-ray observation, ' SPEED-Mk.II'

    NASA Astrophysics Data System (ADS)

    Katsura, Tomoo; Funakoshi, Ken-ichi; Kubo, Atsushi; Nishiyama, Norimasa; Tange, Yoshinori; Sueda, Yu-ichiro; Kubo, Tomoaki; Utsumi, Wataru

    2004-06-01

    SPEED-Mk.II, the second KAWAI-type high P- T apparatus for in situ X-ray observation that was installed at the bending magnet beam line at SPring-8, is described. The guide block system was designed so that the change of the relative dimension of the cubic compression space with press load can be minimized by repeated adjustments. The hydraulic system was designed so as to enable smooth compression and decompression. These precise controls should be advantageous for high-pressure generation, especially when sintered diamond (SD) anvils are used. An oscillation system was equipped for the first time in a large volume press, making it possible to obtain high-quality diffraction patterns even when the number of sample grains is limited. The use of the oscillation system also reduces errors in pressure determination that may be caused by insufficient averaging of diffraction angles over grains in a limited diffraction volume, because the oscillating grains should sweep through the 2 θ range that is allowed by the finite widths of the optical slits.

  14. Observations of Chromospheric Anemone Jets with Hinode CaII Broadband Filtergraph and Hida CaII Spectroheliograph

    NASA Astrophysics Data System (ADS)

    Morita, Satoshi; Shibata, Kazunari; Ueno, Satoru; Ichimoto, Kiyoshi; Kitai, Reizaburo; Otsuji, Ken-Ichi

    2010-08-01

    We report on the first simultaneous observations of chromospheric ``anemone'' jets in solar active regions with the Hinode Solar Optical Telescope (SOT) CaII H broadband filtergraph and the CaII K spetroheliograph on the Domeless Solar Telescope (DST) at Hida Observatory. During the period of coordinated observations, nine chromospheric anemone jets were simultaneously observed with the two instruments. These observations revealed three important features: (1) the jets are generated in the lower chromosphere; i.e., these cannot be seen in CaII K3; (2) the length and lifetime of the jets are 0.4-5 Mm and 40-320 s, respectively; (3) the apparent velocity of the jets observed with the SOT is 3-24 km s-1, while the CaII K3 component at the jets shows a blueshift (in 5 events) in the range of 2-6 km s-1. The chromospheric anemone jets are associated with mixed polarity regions, which are either small emerging flux regions or moving magnetic features. It is found that the CaII K line often shows red or blue asymmetry in the K2/K1 component; the footpoint of the jets associated with emerging flux regions often shows a redshift (2-16 km s-1), while the one with moving magnetic features shows a blueshift (˜5 km s-1). A detailed analysis of the magnetic evolution of the jet-forming regions revealed that the reconnection rate (or canceling rate) of the total magnetic flux at the footpoint of the jets is on the order of 1016 Mx s-1, and the resulting magnetic energy release rate is (1.1-10) × 1024 erg s-1, with a total energy release of (1-13) × 1026 erg for the duration of the magnetic cancellation, ˜130 s. These are comparable to the estimated total energy, ˜1026 erg, in a single chromospheric anemone jet. In addition to the DST CaII K spectroheliogram and the SOT CaII H broadband filtergram, we also used for analysis an SOT magnetogram as well as a Hida Hα filtergram. We present a physical model of the jet based on the observation, and discuss the relation between

  15. Nerve-mediated antidiuresis and antinatriuresis after air-jet stress is modulated by angiotensin II.

    PubMed

    Veelken, R; Hilgers, K F; Stetter, A; Siebert, H G; Schmieder, R E; Mann, J F

    1996-11-01

    A putative interaction between angiotensin II (Ang II) and the sympathetic nervous system within the kidney has been reported. We tested the hypothesis in conscious rats that endogenous Ang II modulates the renal effects of a stress-induced increase in sympathetic nerve activity. We recorded mean arterial blood pressure, heart rate, renal sympathetic nerve activity, renal hemodynamics, urine volume, and urinary sodium content in conscious rats. We used the Ang II type 1 receptor blocker ZD 7155 to inhibit the effects of endogenous Ang II. Ten minutes of air-jet stress increased renal sympathetic nerve activity by 98 +/- 4% (n = 6) without changing systemic hemodynamics. Air-jet stress reduced urine volume (from 31 +/- 3 to 8 +/- 4 microL/min per gram kidney weight, P < .05, n = 12) and sodium excretion (from 4.3 +/- 0.9 to 1.2 +/- 0.3 mumol/min per gram kidney weight, P < .05, n = 12). After renal denervation, air-jet stress had no effect on either parameter. Six micrograms of the Ang II type 1 receptor inhibitor ZD 7155 blunted the decrease in urine volume and sodium excretion in response to air-jet stress, although the increase in renal sympathetic nerve activity during air-jet stress and the pressor response to exogenous Ang II were not affected. Glomerular filtration rate and renal plasma flow were also not affected. Higher doses of 30 and 60 micrograms ZD 7155 inhibited the pressor response to exogenous Ang II and abolished the changes in urine volume and sodium excretion in response to air-jet stress. None of the ZD 7155 doses affected urinary sodium excretion permanently. Hence, the Ang II type 1 receptor antagonist ZD 7155 impaired or abolished the renal nerve-mediated antinatriuresis and anitidiuresis in response to air-jet stress. We conclude that endogenous Ang II modulates the renal effects of centrally mediated changes of sympathetic nerve activity in conscious rats. PMID:8901830

  16. Chromospheric Anemone Jets Observed with Hinode/SOT and Hida Ca II Spectroheliograph

    NASA Astrophysics Data System (ADS)

    Morita, S.; Shibata, K.; Ueno, S.; Ichimoto, K.; Kitai, R.; Otsuji, K.

    2012-08-01

    We present the first simultaneous observations of chromospheric “anemone” jets in active regions with the Ca II H broadband filetergram on the Hinode/SOT and with the Ca II K spetroheliogram on the Domeless Solar Telescope (DST) at the Hida Observatory. During coordinated observation period, 9 chromospheric anemone jets were simultaneously observed with the two instruments. These observations revealed: (1) the jets are generated in the low chromosphere because these cannot be seen in Ca II K3, (2) these jets are associated with mixed polarity regions which are either small emerging flux regions or moving magnetic features, (3) the Ca II K line often show red or blue asymmetry in K2/K1 component; the footpoint of the jets associated with emerging flux regions often show red asymmetry (2-16 km s-1), while the one with moving magnetic features show blue asymmetry (˜5 km s-1). The magnetic cancellations were observed at the footpoint of the jets. The canceling rates are of order of 1016 Mx s-1, and the resulting magnetic energy release rate (1.1-10)×1024 erg s-1, with the total energy release (1-13)×1026 erg for the duration of the magnetic cancellations, ˜130 s. These are comparable to the estimated total energy, ˜1026 erg, in a single chromospheric anemone jet.

  17. Performance oriented packaging testing of nine Mk 3 Mod 0 signal containers in PPP-B-621 wood box for packing group II solid hazardous materials. Final report

    SciTech Connect

    Libbert, K.J.

    1992-10-01

    A PPP-B-621 wood box containing nine Mk 3 Mod 0 Signal containers was tested for conformance to Performance Oriented Packaging criteria established by Code of Federal Regulations Title 49 CFR. The container was tested with a gross weight of 123.3 pounds (56 kilograms) and met all requirements.

  18. Development and Testing of a Jet Assisted Polycrystalline Diamond Drilling Bit. Phase II Development Efforts

    SciTech Connect

    David S. Pixton

    1999-09-20

    Phase II efforts to develop a jet-assisted rotary-percussion drill bit are discussed. Key developments under this contract include: (1) a design for a more robust polycrystalline diamond drag cutter; (2) a new drilling mechanism which improves penetration and life of cutters; and (3) a means of creating a high-pressure mud jet inside of a percussion drill bit. Field tests of the new drill bit and the new robust cutter are forthcoming.

  19. [Fe II] Emission Tracing Massive, Irradiated Jets from Intermediate-Mass Protostars in the Carina Nebula

    NASA Astrophysics Data System (ADS)

    Reiter, Megan; Smith, Nathan

    2013-07-01

    We present new spectroscopy and HST and ground-based AO imaging of five protostellar jets in the Carina nebula. Near-IR [Fe II] emission traces dense gas in the jet that is self-shielded from Lyman continuum photons from nearby O-type stars, but is excited by non-ionizing FUV photons that penetrate the ionization front within the jet. New near-IR [Fe II] images reveal a substantial mass of dense, neutral gas that is not seen in Halpha emission from these jets, leading to densities and mass-loss rate estimates an order of magnitude higher than those derived from the Halpha emission measure. Higher jet mass-loss rates require higher accretion rates, implying that these jets are driven by intermediate-mass (~2-8 Msun) protostars. For two of the sources, mid-IR luminosities of the driving sources are clearly consistent with intermediate-mass protostars, while the other two driving sources are more deeply embedded and require imaging at longer wavelengths with high spatial resolution to confirm their luminosity. Tangential velocities from new proper motion measurements exceed velocities typical for lower-luminosity sources (100-200 km/s). In addition, these outflows are highly collimated, with opening angles of only a few degrees, similar to low-mass protostars. We propose that these jets reflect essentially the same outflow phenomenon seen in low-mass protostars, but that the collimated atomic jet core is irradiated and rendered observable. Thus, the jets in Carina constitute a new view of collimated jets from intermediate-mass protostars that exists in a feedback dominated environment, and offer strong additional evidence that stars up to ~8 Msun form by the same accretion mechanisms as low-mass stars.

  20. Measurement of the inclusive jet cross section using the midpoint algorithm in Run II at CDF

    SciTech Connect

    Group, Robert Craig; /Florida U.

    2006-12-01

    A measurement is presented of the inclusive jet cross section using the Midpoint jet clustering algorithm in five different rapidity regions. This is the first analysis which measures the inclusive jet cross section using the Midpoint algorithm in the forward region of the detector. The measurement is based on more than 1 fb{sup -1} of integrated luminosity of Run II data taken by the CDF experiment at the Fermi National Accelerator Laboratory. The results are consistent with the predictions of perturbative quantum chromodynamics.

  1. Dynamic characteristics of peripheral jet ACV. II - Pitching motion

    NASA Astrophysics Data System (ADS)

    Mori, T.; Maeda, H.

    The dynamic pitching characteristics of peripheral jet ACV (Air Cushion Vehicle) which have a stability curtain are investigated analytically and experimentally. The measured values of moment, lift and cushion pressure are compared with numerical results noting applicability to the pitching motion. The response of ACV to the sinusoidal pitching oscillation of the ground is also studied.

  2. Triggering on B-jets at CDF II

    SciTech Connect

    Amerio, Silvia; Casarsa, Massimo; Cortiana, Giorgio; Donini, Julien; Lucchesi, Donatella; Pagan Griso, Simone; /Padua U. /INFN, Padua

    2009-01-01

    In this paper we present a trigger algorithm able to select online events enriched of b-jets. This feature is of central interest in order to extend the physics reach for standard model and minimal super symmetric model Higgs decaying into a pair of b-quarks. The algorithm fully exploits the recently upgraded CDFII tracking system and Level 2 CALorimeter cluster finder. These upgrades are necessary to cope with Tevatron increasing luminosity and provide new and refined trigger primitives that are the key elements of our algorithm together with the already existing silicon vertex trigger. A b-hadron can travel some millimeters before decaying and the trigger algorithm exploits this characteristic by searching for tracks displaced with respect to the primary vertex and matched to energetic jets of particles. We discuss the study and the optimization of the algorithm, its technical implementation as well as its performance. The new trigger provides an efficient selection for Higgs decaying into a pair of b-quarks and runs up to high luminosity with an acceptable occupancy of the available bandwidth.

  3. Jet-gas interactions and hotspots in FR I/II transition sources

    NASA Astrophysics Data System (ADS)

    Worrall, Diana; Birkinshaw, Mark

    2016-07-01

    Sources with intermediate FR I/II morphologies, and with powers in the decade straddling the FR I/II boundary, provide an opportunity to understand triggers responsible for the different workings of the two classes. Illustrated by deep Chandra observations of several sources, this presentation will show evidence that the physics changes within the transition range, and the work done in driving shocks can exceed that in evacuating the cavities common in FR I sources. Hotspots can be absent, seen only on one side (jet-side or counter-jet-side), or both, in which case X-ray/radio correspondence can be very different on the two sides. Evidence will be shown for radio-emitting plasma running along boundaries between gas of different temperature, apparently lubricating the gas flows and inhibiting heat transfer.

  4. AGN jet power, formation of X-ray cavities, and FR I/II dichotomy in galaxy clusters

    NASA Astrophysics Data System (ADS)

    Fujita, Yutaka; Kawakatu, Nozomu; Shlosman, Isaac

    2016-04-01

    We investigate the ability of jets in active galactic nuclei to break out of the ambient gas with sufficiently large advance velocities. Using observationally estimated jet power, we analyze 28 bright elliptical galaxies in nearby galaxy clusters. Because the gas density profiles in the innermost regions of galaxies have not been resolved so far, we consider two extreme cases for temperature and density profiles. We also follow two types of evolution for the jet cocoons: being driven by the pressure inside the cocoon [Fanaroff-Riley (FR) type I], and being driven by the jet momentum (FR type II). Our main result is that regardless of the assumed form of the density profiles, jets with observed powers of ≲1044 erg s-1 are not powerful enough to evolve as FR II sources. Instead, they evolve as FR I sources and appear to be decelerated below the buoyant velocities of the cocoons when jets were propagating through the central dense regions of the host galaxies. This explains why FR I sources are more frequent than FR II sources in clusters. Furthermore, we predict the sizes of X-ray cavities from the observed jet powers and compare them with the observed ones-they are consistent within a factor of two if the FR I type evolution is realized. Finally, we find that the jets with a power ≳1044 erg s-1 are less affected by the ambient medium, and some of them, but not all, could serve as precursors of the FR II sources.

  5. A Phase II Dose-Ranging Study Evaluating the Efficacy and Safety of the Orexin Receptor Antagonist Filorexant (MK-6096) in Patients with Primary Insomnia

    PubMed Central

    Mahoney, Erin; Jackson, Saheeda; Hutzelmann, Jill; Zhao, Xin; Jia, Nan; Snyder, Ellen; Snavely, Duane; Michelson, David; Roth, Thomas; Herring, W. Joseph

    2016-01-01

    Background: Filorexant (MK-6096) is an orexin receptor antagonist; here, we evaluate the efficacy of filorexant in the treatment of insomnia in adults. Methods: A double-blind, placebo-controlled, randomized, two 4-week–period, adaptive crossover polysomnography study was conducted at 51 sites worldwide. Patients (18 to <65 years) with insomnia received 1 of 4 doses of oral filorexant (2.5, 5, 10, 20mg) once daily at bedtime during one period and matching placebo in the other period in 1 of 8 possible treatment sequences. Polysomnography was performed on night 1 and end of week 4 of each period. The primary endpoint was sleep efficiency at night 1 and end of week 4. Secondary endpoints included wakefulness after persistent sleep onset and latency to onset of persistent sleep. Results: A total of 324 patients received study treatment, 315 received ≥1 dose of placebo, and 318 ≥1 dose of filorexant (2.5mg, n=79; 5mg, n=78; 10mg, n=80; 20mg, n=81). All filorexant doses (2.5/5/10/20mg) were significantly superior to placebo in improving sleep among patients with insomnia as measured by sleep efficiency and wakefulness after persistent sleep onset on night 1 and end of week 4. The 2 higher filorexant doses (10/20mg) were also significantly more effective than placebo in improving sleep onset as measured by latency to onset of persistent sleep at night 1 and end of week 4. Filorexant was generally well tolerated. Conclusions: Orexin receptor antagonism by filorexant significantly improved sleep efficiency in nonelderly patients with insomnia. Dose-related improvements in sleep onset and maintenance outcomes were also observed with filorexant. PMID:26979830

  6. Precise measurement of the top quark mass in the lepton+jets topology at CDF II

    SciTech Connect

    Abulencia, A.; Adelman, J.; Affolder, T.; Akimoto, T.; Albrow, M.G.; Amerio, S.; Amidei, D.; Anastassov, A.; Anikeev, K.; Annovi, A.; Antos, J.; /Comenius U. /Tsukuba U.

    2007-03-01

    The authors present a measurement of the mass of the top quark from proton-antiproton collisions recorded at the CDF experiment in Run II of the Fermilab Tevatron. They analyze events from the single lepton plus jets final state (t{bar t} {yields} W{sup +}bW{sup -}{bar b} {yields} lvbq{bar q}{bar b}). The top quark mass is extracted using a direct calculation of the probability density that each event corresponds to the t{bar t} final state. The probability is a function of both the mass of the top quark and the energy scale of the calorimeter jets, which is constrained in situ by the hadronic W boson mass. Using 167 events observed in 955 pb{sup -1} of integrated luminosity, they achieve the single most precise measurement of the top quark mass, 170.8 {+-} 2.2(stat.) {+-} 1.4(syst.) GeV/c{sup 2}.

  7. HH 666: different kinematics from H α and [Fe II] emission provide a missing link between jets and outflows

    NASA Astrophysics Data System (ADS)

    Reiter, Megan; Smith, Nathan; Kiminki, Megan M.; Bally, John

    2015-06-01

    HH 666 is an externally irradiated protostellar outflow in the Carina nebula for which we present new near-IR [Fe II] spectra obtained with the Folded-Port Infrared Echellette spectrograph at Magellan Observatory. Earlier H α and near-IR [Fe II] imaging revealed that the two emission lines trace substantially different morphologies in the inner ˜40 arcsec of the outflow. H α traces a broad cocoon that surrounds the collimated [Fe II] jet that extends throughout the parent dust pillar. New spectra show that this discrepancy extends to their kinematics. Near-IR [Fe II] emission traces steady, fast velocities of ±200 km s-1 from the eastern and western limbs of the jet. We compare this to a previously published H α spectrum that reveals a Hubble-flow velocity structure near the jet-driving source. New, second-epoch Hubble Space Telescope (HST)/Advanced Camera for Surveys (ACS) H α images reveal the lateral spreading of the H α outflow lobe away from the jet axis. H α proper motions also indicate a sudden increase in the mass-loss rate ˜1000 yr ago, while steady [Fe II] emission throughout the inner jet suggest that the burst is ongoing. An accretion burst sustained for ˜1000 yr is an order of magnitude longer than expected for FU Orionis outbursts, but represents only a small fraction of the total age of the HH 666 outflow. Altogether, available data suggests that [Fe II] traces the highly collimated protostellar jet while H α traces the entrained and irradiated outflow. HH 666 appears to be a missing link between bare jets seen in H II regions and entrained molecular outflows seen from embedded protostars in more quiescent regions.

  8. HST/WFC3 imaging of protostellar jets in Carina: [Fe II] emission tracing massive jets from intermediate-mass protostars

    NASA Astrophysics Data System (ADS)

    Reiter, Megan; Smith, Nathan

    2013-08-01

    We present narrow-band Wide Field Camera 3 (WFC3)-UVIS and WFC3-IR images of four externally irradiated protostellar jets in the Carina nebula: HH 666, HH 901, HH 902 and HH 1066. These massive jets are unusual because they are bathed in UV radiation from dozens of nearby O-type stars, but despite the strong incident ionizing radiation, portions of the jet remain neutral. Near-IR [Fe II] images reveal dense, neutral gas that was not seen in previous studies of Hα emission. We show that near-IR [Fe II] emitting gas must be self-shielded from Lyman continuum photons, regardless of its excitation mechanism (shocks, far-ultraviolet radiation or both). High densities are required for the survival of Fe+ amid the strong Lyman continuum luminosity from Tr14, raising estimates of the mass-loss rates by an order of magnitude. Higher jet mass-loss rates require higher accretion rates on to their driving protostars, implying that these jets are driven by intermediate-mass (˜2-8 M⊙) stars. Indeed, the IR driving sources of two of these outflows have luminosities that require intermediate-mass protostars (the other two are so deeply embedded that their luminosity is uncertain). All four of these HH jets are highly collimated, with opening angles of only a few degrees, similar to those observed in low-mass protostars. We propose that these jets reflect essentially the same outflow phenomenon seen in wide-angle molecular outflows associated with intermediate- and high-mass protostars, but that the collimated atomic jet core is irradiated and rendered observable in the harsh radiative environment of the Carina nebula. In more quiescent environments, this atomic core remains invisible, and outflows traced by shock-excited molecules in the outflow cavity give the impression that these outflows have a wider opening angle. Thus, the externally irradiated jets in Carina constitute a new view of collimated jets from intermediate-mass protostars and offer strong additional evidence

  9. A Direct Top-Quark Width Measurement from Lepton + Jets Events at CDF II

    SciTech Connect

    Aaltonen, T.; Alvarez Gonzalez, B.; Amerio, S.; Amidei, D.; Anastassov, A.; Annovi, A.; Antos, J.; Apollinari, G.; Appel, J.A.; Apresyan, A.; Arisawa, T.; /Waseda U. /Dubna, JINR

    2010-08-01

    We present a measurement of the top-quark width using t{bar t} events produced in p{bar p} collisions at Fermilab's Tevatron collider and collected by the CDF II detector. In the mode where the top quark decays to a W boson and a bottom quark, we select events in which one W decays leptonically and the other hadronically (lepton + jets channel) . From a data sample corresponding to 4.3 fb{sup -1} of integrated luminosity, we identify 756 candidate events. The top-quark mass and the mass of W boson that decays hadronically are reconstructed for each event and compared with templates of different top-quark widths ({Lambda}{sub t}) and deviations from nominal jet energy scale ({Delta}{sub JES}) to perform a simultaneous fit for both parameters, where {Delta}{sub JES} is used for the in situ calibration of the jet energy scale. By applying a Feldman-Cousins approach, we establish an upper limit at 95% confidence level (CL) of {Lambda}{sub t} < 7.6 GeV and a two-sided 68% CL interval of 0.3 GeV < {Lambda}{sub t} < 4.4 GeV for a top-quark mass of 172.5 GeV/c{sup 2}, which are consistant with the standard model prediction. This is the first direct measurement of {Lambda}{sub t} to set a lower limit with 68% CL.

  10. Polarized Atomic Hydrogen Beam Tests in the Mark-II Ultra-Cold Jet Target.

    NASA Astrophysics Data System (ADS)

    Luppov, V. G.; Blinov, B. B.; Gladycheva, S. E.; Kageya, T.; Kantsyrev, D. Yu.; Krisch, A. D.; Murray, J. R.; Neumann, J. J.; Raymond, R. S.; Borisov, N. S.; Kleppner, D.; Davidenko, A. M.; Grishin, V. N.

    2000-04-01

    To study spin effects in high energy collisions, we are developing an ultra-cold high-density jet target of proton-spin-polarized hydrogen atoms (Mark-II). The target uses a 12 Tesla magnetic field and a 0.3 K separation cell coated with superfluid helium-4 to produce a slow monochromatic electron-spin-polarized atomic hydrogen beam; an rf transition unit then converts this into a proton-spin-polarized beam, which is focused by a superconducting sextupole into the interaction region. Recently, the Jet produced a measured electron-spin-polarized atomic hydrogen beam of about 10^15 H s-1 into a 0.3 cm^2 area at the detector. This intensity corresponds to the free jet density of about 10^11 H cm-3 with a proton polarization of about 50%. So far, the intensity is limited by the high insulation vacuum pressure due to the evaporation of the separation cell's helium film. The beam's angular and radial distributions were measured. A test of a new superfluid-^4He-coated parabolic mirror, attached to the separation cell, appeared to increase the beam intensity by a factor of about 3, as expected.

  11. SOLAR X-RAY JETS, TYPE-II SPICULES, GRANULE-SIZE EMERGING BIPOLES, AND THE GENESIS OF THE HELIOSPHERE

    SciTech Connect

    Moore, Ronald L.; Sterling, Alphonse C.; Cirtain, Jonathan W.; Falconer, David A.

    2011-04-10

    From Hinode observations of solar X-ray jets, Type-II spicules, and granule-size emerging bipolar magnetic fields in quiet regions and coronal holes, we advocate a scenario for powering coronal heating and the solar wind. In this scenario, Type-II spicules and Alfven waves are generated by the granule-size emerging bipoles (EBs) in the manner of the generation of X-ray jets by larger magnetic bipoles. From observations and this scenario, we estimate that Type-II spicules and their co-generated Alfven waves carry into the corona an area-average flux of mechanical energy of {approx}7 x 10{sup 5} erg cm{sup -2} s{sup -1}. This is enough to power the corona and solar wind in quiet regions and coronal holes, and therefore indicates that the granule-size EBs are the main engines that generate and sustain the entire heliosphere.

  12. Chandra Reveals Twin X-ray Jets in the Powerful FR-II Radio Galaxy 3C353

    SciTech Connect

    Kataoka, J.; Stawarz, L.; Harris, D.E.; Siemiginowska, A.; Ostrowski, M.; Swain, M.R.; Hardcastle, M.J.; Goodger, J.L.; Iwasawa, K.; Edwards, P.G.

    2008-06-13

    We report X-ray imaging of the powerful FR II radio galaxy 3C 353 using the Chandra X-ray Observatory. 3C 353's two 4-inch wide and 2-feet long jets allow us to study in detail the internal structure of the large-scale relativistic outflows at both radio and X-ray photon energies with the sub-arcsecond spatial resolution provided by the VLA and Chandra instruments. In a 90 ks Chandra observation, we have detected X-ray emission from most radio structures in 3C 353, including the nucleus, the jet and the counterjet, the terminal jet regions (hotspots), and one radio lobe. We show that the detection of the X-ray emission associated with the radio knots and counterknots, which is most likely non-thermal in origin, puts several crucial constraints on the X-ray emission mechanisms in powerful large-scale jets of quasars and FR II sources. In particular, we show that this detection is inconsistent with the inverse-Compton model proposed in the literature, and instead implies a synchrotron origin of the X-ray jet photons. We also find that the width of the X-ray counterjet is possibly narrower than that measured in radio bands, that the radio-to-X-ray flux ratio decreases systematically downstream along the jets, and that there are substantial (kpc-scale) offsets between the positions of the X-ray and radio intensity maxima within each knot, whose magnitudes increase away from the nucleus. We discuss all these findings in the wider context of the physics of extragalactic jets, proposing some particular though not definitive solutions or interpretations for each problem. In general, we find that the synchrotron X-ray emission of extragalactic large-scale jets is not only shaped by the global hydrodynamical configuration of the outflows, but is also likely to be very sensitive to the microscopic parameters of the jet plasma. A complete, self-consistent model for the X-ray emission of extragalactic jets still remains elusive.

  13. Chandra Reveals Twin X-ray Jets in the Powerful FR II Radio Galaxy 3C 353

    SciTech Connect

    Kataoka, Jun

    2008-12-24

    We report X-ray imaging of the powerful FR II radio galaxy 3C 353 using the Chandra X-ray Observatory. 3C 353's two 4''-wide and 2'-long jets allow us to study in detail the internal structure of the large-scale relativistic outflows at both radio and X-ray photon energies with the sub-arcsecond spatial resolution provided by the VLA and Chandra instruments. In a 90 ks Chandra observation, we have detected X-ray emission from most radio structures in 3C 353, including the nucleus, the jet and the counterjet, the terminal jet regions (hotspots), and one radio lobe. We show that the detection of the X-ray emission associated with the radio knots and counterknots, which is most likely non-thermal in origin, puts several crucial constraints on the X-ray emission mechanisms in powerful large-scale jets of quasars and FR II sources. In particular, we show that this detection is inconsistent with the inverse-Compton model proposed in the literature, and instead implies a synchrotron origin of the X-ray jet photons. We also find that the width of the X-ray counterjet is possibly narrower than that measured in radio bands, that the radio-to-X-ray flux ratio decreases systematically downstream along the jets, and that there are substantial (kpc-scale) offsets between the positions of the X-ray and radio intensity maxima within each knot, whose magnitudes increase away from the nucleus. We discuss all these findings in the wider context of the physics of extragalactic jets, proposing some particular though not definitive solutions or interpretations for each problem. In general, we find that the synchrotron X-ray emission of extragalactic large-scale jets is not only shaped by the global hydrodynamical configuration of the outflows, but is also likely to be very sensitive to the microscopic parameters of the jet plasma. A complete, self-consistent model for the X-ray emission of extragalactic jets still remains elusive.

  14. The First measurement of the top quark mass at CDF II in the lepton+jets and dilepton channels simultaneously

    SciTech Connect

    Aaltonen, T.; Adelman, J.; Akimoto, T.; Albrow, Michael G.; Alvarez Gonzalez, B.; Amerio, S.; Amidei, Dante E.; Anastassov, A.; Annovi, Alberto; Antos, J.; Apollinari, G.; /Fermilab /Purdue U.

    2008-09-01

    The authors present a measurement of the mass of the top quark using data corresponding to an integrated luminosity of 1.9 fb{sup -1} of p{bar p} collisions collected at {radical}s = 1.96 TeV with the CDF II detector at Fermilab's Tevatron. This is the first measurement of the top quark mass using top-antitop pair candidate events in the lepton + jets and dilepton decay channels simultaneously. They reconstruct two observables in each channel and use a non-parametric kernel density estimation technique to derive two-dimensional probability density functions from simulated signal and background samples. The observables are the top quark mass and the invariant mass of two jets from the W decay in the lepton + jets channel, and the top quark mass and the scalar sum of transverse energy of the event in the diletpon channel. They perform a simultaneous fit for the top quark mass and the jet energy scale, which is constrained in situ by the hadronic W boson mass. using 332 lepton + jets candidate events and 144 diletpon candidate events, they measure the top quark mass to be m{sub top} = 171.9 {+-} 1.7 (stat. + JES) {+-} 1.1 (other sys.) GeV/c{sup 2} = 171.9 {+-} 2.0 GeV/c{sup 2}.

  15. Measurement of the W Plus N Inclusive Jets Cross-Section at CDF Run II

    SciTech Connect

    Stentz, Dale James

    2012-01-01

    In this thesis we present the study of the production of the W boson in association with hadronic jets at the Collider Detector at Fermilab (CDF). Along with the electroweak properties the W boson, we examine jet kinematic variables with the aim of studying predictions of perturbative quantum chromodynamics. We derive several di erential crosssections as a function of the inclusive jet multiplicity and the transverse momenta of each jet. In this analysis, we are using 2.8 fb-1 of data and consider both the electron and muon lepton nal states for the W boson decay.

  16. Sub-arcsecond [Fe ii] spectro-imaging of the DG Tauri jet. Periodic bubbles and a dusty disk wind?

    NASA Astrophysics Data System (ADS)

    Agra-Amboage, V.; Dougados, C.; Cabrit, S.; Reunanen, J.

    2011-08-01

    Context. The origin of protostellar jets as well as their impact on the regulation of angular momentum and the inner disk physics are still crucial open questions in star formation. Aims: We aim to test the different proposed ejection processes in T Tauri stars through high-angular resolution observations of forbidden-line emission from the inner DG Tauri microjet. Methods: We present spectro-imaging observations of the DG Tauri jet obtained with SINFONI/VLT in the lines of [Fe ii]λ1.64 μm, 1.53 μm with 0.15 arcsec angular resolution and R = 3000 spectral resolution. We analyze the morphology and kinematics, derive electronic densities and mass-flux rates and discuss the implications for proposed jet launching models. Results: (1) We observe an onion-like velocity structure in [Fe ii] in the blueshifted jet, similar to that observed in optical lines. High-velocity (HV) gas at ≃ -200 km s-1 is collimated inside a half-opening angle of 4° and medium-velocity (MV) gas at ≃ -100 km s-1 in a cone with an half-opening angle 14° (2) Two new axial jet knots are detected in the blue jet, as well as a more distant bubble with corresponding counter-bubble. The periodic knot ejection timescale is revised downward to 2.5 yrs. (3) The redshifted jet is detected only beyond 0.7 arcsec from the star, yielding revised constraints on the disk surface density. (4) From comparison to [O i] data we infer iron depletion of a factor 3 at high velocities and a factor 10 at speeds below -100 km s-1. (5) The mass-fluxes in each of the medium and high-velocity components of the blueshifted lobe are ≃1.6 ± 0.8 × 10-8 M⊙ yr-1, representing 0.02 - 0.2 of the disk accretion rate. Conclusions: The medium-velocity conical [Fe ii] flow in the DG Tau jet is too fast and too narrow to trace photo-evaporated matter from the disk atmosphere. Both its kinematics and collimation cannot be reproduced by the X-wind, nor can the "conical magnetospheric wind". The level of Fe gas phase

  17. PAGaN II: The Evolution of AGN Jets on Sub-Parsec Scales

    NASA Astrophysics Data System (ADS)

    Oh, Junghwan; Trippe, Sascha; Kang, Sincheol; Kim, Jae-Young; Park, Jong-Ho; Lee, Taeseok; Kim, Daewon; Kino, Motoki; Lee, Sang-Sung; Sohn, Bong Won

    2015-10-01

    We report first results from KVN and VERA Array (KaVA) VLBI observations obtained in the frame of our Plasma-physics of Active Galactic Nuclei (PAGaN) project. We observed eight selected AGN at 22 and 43 GHz in single polarization (LCP) between March 2014 and April 2015. Each source was observed for 6 to 8 hours per observing run to maximize the uv coverage. We obtained a total of 15 deep high-resolution images permitting the identification of individual circular Gaussian jet components and three spectral index maps of BL Lac, 3C~111 and 3C~345 from simultaneous dual-frequency observations. The spectral index maps show trends in agreement with general expectations -- flat core and steep jets -- while the actual value of the spectral index for jets shows indications for a dependence on AGN type. We analyzed the kinematics of jet components of BL Lac and 3C~111, detecting superluminal proper motions with maximum apparent speeds of about 5c. This constrains the lower limits of the intrinsic component velocities to ˜0.98c and the upper limits of the angle between jet and line of sight to ˜20°. In agreement with global jet expansion, jet components show systematically larger diameters d at larger core distances r, following the global relation d≈0.2r, albeit within substantial scatter.

  18. Precise measurement of the top-quark mass in the lepton+jets topology at CDF II.

    PubMed

    Aaltonen, T; Abulencia, A; Adelman, J; Affolder, T; Akimoto, T; Albrow, M G; Amerio, S; Amidei, D; Anastassov, A; Anikeev, K; Annovi, A; Antos, J; Aoki, M; Apollinari, G; Arisawa, T; Artikov, A; Ashmanskas, W; Attal, A; Aurisano, A; Azfar, F; Azzi-Bacchetta, P; Azzurri, P; Bacchetta, N; Badgett, W; Barbaro-Galtieri, A; Barnes, V E; Barnett, B A; Baroiant, S; Bartsch, V; Bauer, G; Beauchemin, P-H; Bedeschi, F; Behari, S; Bellettini, G; Bellinger, J; Belloni, A; Benjamin, D; Beretvas, A; Beringer, J; Berry, T; Bhatti, A; Binkley, M; Bisello, D; Bizjak, I; Blair, R E; Blocker, C; Blumenfeld, B; Bocci, A; Bodek, A; Boisvert, V; Bolla, G; Bolshov, A; Bortoletto, D; Boudreau, J; Boveia, A; Brau, B; Brigliadori, L; Bromberg, C; Brubaker, E; Budagov, J; Budd, H S; Budd, S; Burkett, K; Busetto, G; Bussey, P; Buzatu, A; Byrum, K L; Cabrera, S; Campanelli, M; Campbell, M; Canelli, F; Canepa, A; Carrillo, S; Carlsmith, D; Carosi, R; Carron, S; Casal, B; Casarsa, M; Castro, A; Catastini, P; Cauz, D; Cavalli-Sforza, M; Cerri, A; Cerrito, L; Chang, S H; Chen, Y C; Chertok, M; Chiarelli, G; Chlachidze, G; Chlebana, F; Cho, I; Cho, K; Chokheli, D; Chou, J P; Choudalakis, G; Chuang, S H; Chung, K; Chung, W H; Chung, Y S; Cilijak, M; Ciobanu, C I; Ciocci, M A; Clark, A; Clark, D; Coca, M; Compostella, G; Convery, M E; Conway, J; Cooper, B; Copic, K; Cordelli, M; Cortiana, G; Crescioli, F; Cuenca Almenar, C; Cuevas, J; Culbertson, R; Cully, J C; DaRonco, S; Datta, M; D'Auria, S; Davies, T; Dagenhart, D; de Barbaro, P; De Cecco, S; Deisher, A; De Lentdecker, G; De Lorenzo, G; Dell'Orso, M; Delli Paoli, F; Demortier, L; Deng, J; Deninno, M; De Pedis, D; Derwent, P F; Di Giovanni, G P; Dionisi, C; Di Ruzza, B; Dittmann, J R; D'Onofrio, M; Dörr, C; Donati, S; Dong, P; Donini, J; Dorigo, T; Dube, S; Efron, J; Erbacher, R; Errede, D; Errede, S; Eusebi, R; Fang, H C; Farrington, S; Fedorko, I; Fedorko, W T; Feild, R G; Feindt, M; Fernandez, J P; Field, R; Flanagan, G; Forrest, R; Forrester, S; Franklin, M; Freeman, J C; Furic, I; Gallinaro, M; Galyardt, J; Garcia, J E; Garberson, F; Garfinkel, A F; Gay, C; Gerberich, H; Gerdes, D; Giagu, S; Giannetti, P; Gibson, K; Gimmell, J L; Ginsburg, C; Giokaris, N; Giordani, M; Giromini, P; Giunta, M; Giurgiu, G; Glagolev, V; Glenzinski, D; Gold, M; Goldschmidt, N; Goldstein, J; Golossanov, A; Gomez, G; Gomez-Ceballos, G; Goncharov, M; González, O; Gorelov, I; Goshaw, A T; Goulianos, K; Gresele, A; Grinstein, S; Grosso-Pilcher, C; Group, R C; Grundler, U; Guimaraes da Costa, J; Gunay-Unalan, Z; Haber, C; Hahn, K; Hahn, S R; Halkiadakis, E; Hamilton, A; Han, B-Y; Han, J Y; Handler, R; Happacher, F; Hara, K; Hare, D; Hare, M; Harper, S; Harr, R F; Harris, R M; Hartz, M; Hatakeyama, K; Hauser, J; Hays, C; Heck, M; Heijboer, A; Heinemann, B; Heinrich, J; Henderson, C; Herndon, M; Heuser, J; Hidas, D; Hill, C S; Hirschbuehl, D; Hocker, A; Holloway, A; Hou, S; Houlden, M; Hsu, S-C; Huffman, B T; Hughes, R E; Husemann, U; Huston, J; Incandela, J; Introzzi, G; Iori, M; Ivanov, A; Iyutin, B; James, E; Jang, D; Jayatilaka, B; Jeans, D; Jeon, E J; Jindariani, S; Johnson, W; Jones, M; Joo, K K; Jun, S Y; Jung, J E; Junk, T R; Kamon, T; Karchin, P E; Kato, Y; Kemp, Y; Kephart, R; Kerzel, U; Khotilovich, V; Kilminster, B; Kim, D H; Kim, H S; Kim, J E; Kim, M J; Kim, S B; Kim, S H; Kim, Y K; Kimura, N; Kirsch, L; Klimenko, S; Klute, M; Knuteson, B; Ko, B R; Kondo, K; Kong, D J; Konigsberg, J; Korytov, A; Kotwal, A V; Kraan, A C; Kraus, J; Kreps, M; Kroll, J; Krumnack, N; Kruse, M; Krutelyov, V; Kubo, T; Kuhlmann, S E; Kuhr, T; Kulkarni, N P; Kusakabe, Y; Kwang, S; Laasanen, A T; Lai, S; Lami, S; Lammel, S; Lancaster, M; Lander, R L; Lannon, K; Lath, A; Latino, G; Lazzizzera, I; LeCompte, T; Lee, J; Lee, J; Lee, Y J; Lee, S W; Lefèvre, R; Leonardo, N; Leone, S; Levy, S; Lewis, J D; Lin, C; Lin, C S; Lindgren, M; Lipeles, E; Lister, A; Litvintsev, D O; Liu, T; Lockyer, N S; Loginov, A; Loreti, M; Lu, R-S; Lucchesi, D; Lujan, P; Lukens, P; Lungu, G; Lyons, L; Lys, J; Lysak, R; Lytken, E; Mack, P; MacQueen, D; Madrak, R; Maeshima, K; Makhoul, K; Maki, T; Maksimovic, P; Malde, S; Malik, S; Manca, G; Manousakis, A; Margaroli, F; Marginean, R; Marino, C; Marino, C P; Martin, A; Martin, M; Martin, V; Martínez, M; Martínez-Ballarín, R; Maruyama, T; Mastrandrea, P; Masubuchi, T; Matsunaga, H; Mattson, M E; Mazini, R; Mazzanti, P; McFarland, K S; McIntyre, P; McNulty, R; Mehta, A; Mehtala, P; Menzemer, S; Menzione, A; Merkel, P; Mesropian, C; Messina, A; Miao, T; Miladinovic, N; Miles, J; Miller, R; Mills, C; Milnik, M; Mitra, A; Mitselmakher, G; Miyamoto, A; Moed, S; Moggi, N; Mohr, B; Moon, C S; Moore, R; Morello, M; Movilla Fernandez, P; Mülmenstädt, J; Mukherjee, A; Muller, Th; Mumford, R; Murat, P; Mussini, M; Nachtman, J; Nagano, A; Naganoma, J; Nakamura, K; Nakano, I; Napier, A; Necula, V; Neu, C; Neubauer, M S; Nielsen, J; Nodulman, L; Norniella, O; Nurse, E; Oh, S H; Oh, Y D; Oksuzian, I; Okusawa, T; Oldeman, R; Orava, R; Osterberg, K; Pagliarone, C; Palencia, E; Papadimitriou, V; Papaikonomou, A; Paramonov, A A; Parks, B; Pashapour, S; Patrick, J; Pauletta, G; Paulini, M; Paus, C; Pellett, D E; Penzo, A; Phillips, T J; Piacentino, G; Piedra, J; Pinera, L; Pitts, K; Plager, C; Pondrom, L; Portell, X; Poukhov, O; Pounder, N; Prakoshyn, F; Pronko, A; Proudfoot, J; Ptohos, F; Punzi, G; Pursley, J; Rademacker, J; Rahaman, A; Ramakrishnan, V; Ranjan, N; Redondo, I; Reisert, B; Rekovic, V; Renton, P; Rescigno, M; Richter, S; Rimondi, F; Ristori, L; Robson, A; Rodrigo, T; Rogers, E; Rolli, S; Roser, R; Rossi, M; Rossin, R; Roy, P; Ruiz, A; Russ, J; Rusu, V; Saarikko, H; Safonov, A; Sakumoto, W K; Salamanna, G; Saltó, O; Santi, L; Sarkar, S; Sartori, L; Sato, K; Savard, P; Savoy-Navarro, A; Scheidle, T; Schlabach, P; Schmidt, E E; Schmidt, M P; Schmitt, M; Schwarz, T; Scodellaro, L; Scott, A L; Scribano, A; Scuri, F; Sedov, A; Seidel, S; Seiya, Y; Semenov, A; Sexton-Kennedy, L; Sfyrla, A; Shalhout, S Z; Shapiro, M D; Shears, T; Shepard, P F; Sherman, D; Shimojima, M; Shochet, M; Shon, Y; Shreyber, I; Sidoti, A; Sinervo, P; Sisakyan, A; Slaughter, A J; Slaunwhite, J; Sliwa, K; Smith, J R; Snider, F D; Snihur, R; Soderberg, M; Soha, A; Somalwar, S; Sorin, V; Spalding, J; Spinella, F; Spreitzer, T; Squillacioti, P; Stanitzki, M; Staveris-Polykalas, A; St Denis, R; Stelzer, B; Stelzer-Chilton, O; Stentz, D; Strologas, J; Stuart, D; Suh, J S; Sukhanov, A; Sun, H; Suslov, I; Suzuki, T; Taffard, A; Takashima, R; Takeuchi, Y; Tanaka, R; Tecchio, M; Teng, P K; Terashi, K; Thom, J; Thompson, A S; Thomson, E; Tipton, P; Tiwari, V; Tkaczyk, S; Toback, D; Tokar, S; Tollefson, K; Tomura, T; Tonelli, D; Torre, S; Torretta, D; Tourneur, S; Trischuk, W; Tsuno, S; Tu, Y; Turini, N; Ukegawa, F; Uozumi, S; Vallecorsa, S; van Remortel, N; Varganov, A; Vataga, E; Vazquez, F; Velev, G; Vellidis, C; Veramendi, G; Veszpremi, V; Vidal, M; Vidal, R; Vila, I; Vilar, R; Vine, T; Vogel, M; Vollrath, I; Volobouev, I; Volpi, G; Würthwein, F; Wagner, P; Wagner, R G; Wagner, R L; Wagner, J; Wagner, W; Wallny, R; Wang, S M; Warburton, A; Waters, D; Weinberger, M; Wester, W C; Whitehouse, B; Whiteson, D; Wicklund, A B; Wicklund, E; Williams, G; Williams, H H; Wilson, P; Winer, B L; Wittich, P; Wolbers, S; Wolfe, C; Wright, T; Wu, X; Wynne, S M; Yagil, A; Yamamoto, K; Yamaoka, J; Yamashita, T; Yang, C; Yang, U K; Yang, Y C; Yao, W M; Yeh, G P; Yoh, J; Yorita, K; Yoshida, T; Yu, G B; Yu, I; Yu, S S; Yun, J C; Zanello, L; Zanetti, A; Zaw, I; Zhang, X; Zhou, J; Zucchelli, S

    2007-11-01

    We present a measurement of the mass of the top quark from proton-antiproton collisions recorded at the CDF experiment in Run II of the Fermilab Tevatron. We analyze events from the single lepton plus jets final state (tt-->W(+)bW(-)b-->lnubqq'b). The top-quark mass is extracted using a direct calculation of the probability density that each event corresponds to the tt final state. The probability is a function of both the mass of the top quark and the energy scale of the calorimeter jets, which is constrained in situ by the hadronic W boson mass. Using 167 events observed in 955 pb(-1) of integrated luminosity, we achieve the single most precise measurement of the top-quark mass, 170.8+/-2.2(stat.)+/-1.4(syst.) GeV/c(2). PMID:17995397

  19. Neuroprotective effects of MK-801 against traumatic brain injury in immature rats.

    PubMed

    Sönmez, Ataç; Sayın, Oya; Gürgen, Seren Gülşen; Çalişir, Meryem

    2015-06-15

    Traumatic brain injury (TBI) is a major health problem in pediatric ages and also has major social, economic, and emotional outcomes, with diverse sequelae in many spheres of everyday life. We aimed to investigate the effect of MK-801, a competitive NMDA receptor antagonist, on hippocampal damage and behavioral deficits on 10-day-old rat pups subjected to contusion injury. The aims of the present study were to determine: (i) the short term effects of MK-801 on hippocampal BDNF, NGF and NMDA receptor immunoreactivity and neuron density in hippocampus (ii) long term effects of MK-801 on cognitive dysfunction following TBI in the immature rats. MK-801, was injected intraperitoneally at the doses of 1mg/kg of body weight immediately after induction of traumatic injury. Hippocampal damage was examined by cresyl violet staining, BDNF, NGF and NMDAR receptor immunohistochemistry on P10 day and behavioral alterations were evaluated using elevated plus maze and novel object recognition tests two months after the trauma. Histopathological and immunohistochemical evaluations showed that treatment with a single dose of 1mg/kg MK-801 (i.p.) significantly ameliorated the trauma induced hippocampal neuron loss and decreased BDNF, NGF and NMDAR expressions in CA1, CA3 and DG hippocampal brain regions. Additionally, treatment with MK-801 ameliorated anxiety and hippocampus dependent memory of animals subjected to trauma. These results show that acute treatment of MK-801 has a neuroprotective role against trauma induced hippocampal neuron loss and associated cognitive impairment in immature rats. PMID:25943283

  20. Contribution of NMDA, GABAA and GABAB receptors and l-arginine-NO-cGMP, MEK1/2 and CaMK-II pathways in the antidepressant-like effect of 7-fluoro-1,3-diphenylisoquinoline-1-amine in mice.

    PubMed

    Pesarico, Ana Paula; Stangherlin, Eluza Curte; Rosa, Suzan Gonçalves; Mantovani, Anderson C; Zeni, Gilson; Nogueira, Cristina Wayne

    2016-07-01

    It has been reported that the antidepressant-like effect of 7-fluoro-1,3-diphenylisoquinoline-1-amine (FDPI) may result from the modulation of brain monoaminergic systems. However, the mechanisms of FDPI action are not fully understood. The aim of this study was to investigate the contribution of N-methyl-d-aspartate (NMDA) and gamma-aminobutyric acid (GABA) systems as well as l-arginine-nitric oxide-(NO)-cyclic guanosine monophosphate-(cGMP), mitogen-activated protein/extracellular signal-regulated kinase (MEK1/2) and Ca(2+)/calmodulin-dependent protein kinase II (CaMK-II) signaling pathways in the antidepressant-like effect of FDPI in the mouse forced swimming test (FST). The levels of NO and uptake of [(3)H]glutamate and [(3)H]GABA were determined in prefrontal cortices of Swiss mice. Pretreatments with NMDA (0.1 pmol/site, i.c.v., a NMDA receptor agonist), bicuculline (1mg/kg, i.p., a GABAA receptor antagonist), phaclofen (2mg/kg, i.p., a GABAB receptor antagonist) and l-arginine (750mg/kg, i.p., a NO precursor), KN-62 (1μg/site, a CaMK-II inhibitor), U0126 (5μg/site, a MEK1/2 inhibitor) and PD09058 (5μg/site, a MEK1/2 inhibitor) blocked the antidepressant-like effect of FDPI, at a dose of 1mg/kg, in the FST. ODQ (30 pmol/site, i.c.v., a soluble guanylate cyclase (sGC) inhibitor) in combination with a sub-effective dose of FDPI (0.1mg/kg, i.g.) reduced the immobility time in the FST. The administration of FDPI (50mg/kg) to mice increased the glutamate uptake and reduced NO levels in the prefrontal cortex of mice. The results suggest a contribution of NMDA, GABAA and GABAB receptors and l-arginine-NO-cGMP pathway in the antidepressant-like action of FDPI in mice, and this effect is related to CaMK-II and MEK 1/2 activation. PMID:27112660

  1. Near Noise Field of a Jet-engine Exhaust II : Cross Correlation of Sound Pressures

    NASA Technical Reports Server (NTRS)

    Callaghan, Edmund E; Howes, Walton L; Coles, Willard D

    1956-01-01

    Pressure cross correlations were obtained over a range of jet velocities both longitudinally and laterally for the overall sound pressure and for several frequency bands. The region of positive correlation was found to increase with distance downstream of the nozzle exit and was greater for lateral than for longitudinal correlations. In general, little change in the correlation curves was found as a function of jet velocity or frequency band width. Measurements made with a fixed and a movable microphone in a plate showed correlations similar to the free-field results. The results are interpreted in terms of pressure loads on surfaces.

  2. Pilot study of MK-462 in migraine.

    PubMed

    Cutler, N R; Claghorn, J; Sramek, J J; Block, G; Panebianco, D; Cheng, H; Olah, T V; Reines, S A

    1996-04-01

    MK-462 is a potent, selective 5HT1D receptor agonist which may be useful in treating acute migraine. We conducted a double-blind placebo-controlled inpatient study to assess the preliminary efficacy and safety of oral doses of MK-462 20 mg (n = 8) and 40 mg (n = 36) vs placebo (n = 21), administered to 65 male and post-menopausal female migraine patients aged 22-51 with moderate or severe migraine headache. Headache severity and functional disability were measured at 0.5, 1, 1.5, and 2 h post-dose. The 20 mg dose was well tolerated and 4/8 patients obtained relief in headache severity at the 2 h time point. The 40 mg dose was well tolerated and was significantly (p < 0.05) superior to placebo at the 1.5 and 2 h time points (with 27/36 or 75% obtaining relief at 2 h compared to 7/21 or 33% for placebo). Adverse events occurred in 50% of patients on 20 mg MK-462, 72% of those on 40 mg MK-462, and in 52% of placebo-treated subjects. The most common adverse events associated with MK-462 were drowsiness (20 mg 12%; 40 mg 44%; placebo 24%), dry mouth (40 mg 36%; placebo 19%), and lightheadedness/dizziness (40 mg 17%; placebo 10%). Based on these preliminary results, MK-462 appears worthy of continued study for the treatment of acute migraine. PMID:8665577

  3. PERFORMANCE OF A HIGH-VELOCITY PULSE-JET FILTER, II

    EPA Science Inventory

    The report gives results of a study of the performance of a high-velocity pulse-jet filter. Such filtration has distinct advantages over low-velocity filtration in that the equipment required to clean a gas stream is reduced in size and initial cost as velocity increases. Althoug...

  4. Measurement of the Top Quark Mass at D0 Run II with the Matrix Element Method in the Lepton+Jets Final State

    SciTech Connect

    Schieferdecker, Philipp; /Munich U.

    2005-08-01

    The mass of the top quark is a fundamental parameter of the Standard Model. Its precise knowledge yields valuable insights into unresolved phenomena in and beyond the Standard Model. A measurement of the top quark mass with the matrix element method in the lepton+jets final state in D0 Run II is presented. Events are selected requiring an isolated energetic charged lepton (electron or muon), significant missing transverse energy, and exactly four calorimeter jets. For each event, the probabilities to originate from the signal and background processes are calculated based on the measured kinematics, the object resolutions and the respective matrix elements. The jet energy scale is known to be the dominant source of systematic uncertainty. The reference scale for the mass measurement is derived from Monte Carlo events. The matrix element likelihood is defined as a function of both, m{sub top} and jet energy scale JES, where the latter represents a scale factor with respect to the reference scale. The top mass is obtained from a two-dimensional correlated fit, and the likelihood yields both the statistical and jet energy scale uncertainty. Using a dataset of 320 pb{sup -1} of D0 Run II data, the mass of the top quark is measured to be: m{sub top}{sup {ell}+jets} = 169.5 {+-} 4.4(stat. + JES){sub -1.6}{sup +1.7}(syst.) GeV; m{sub top}{sup e+jets} = 168.8 {+-} 6.0(stat. + JES){sub -1.9}{sup +1.9}(syst.) GeV; m{sub top}{sup {mu}+jets} = 172.3 {+-} 9.6(stat.+JES){sub -3.3}{sup +3.4}(syst.) GeV. The jet energy scale measurement in the {ell}+jets sample yields JES = 1.034 {+-} 0.034, suggesting good consistency of the data with the simulation. The measurement forecasts significant improvements to the total top mass uncertainty during Run II before the startup of the LHC, as the data sample will grow by a factor of ten and D0's tracking capabilities will be employed in jet energy reconstruction and flavor identification.

  5. Altitude-chamber performance of British Rolls-Royce Nene II engine III : 18.00-inch-diameter jet nozzle

    NASA Technical Reports Server (NTRS)

    Grey, Ralph E; Brightwell, Virginia L; Barson, Zelmar; NACA

    1950-01-01

    An altitude-chamber investigation of British Rolls-Royce Nene II turbojet engine was conducted over range of altitudes from sea level to 65,000 feet and ram pressure ratios from 1.10 to 3.50, using an 18.00-inch-diameter jet nozzle. The 18.00-inch-diameter jet nozzle gave slightly lower values of net-thrust specific fuel consumption than either the 18.41- or the standard 18.75-inch-diameter jet nozzles at high flight speeds. At low flight speeds, the 18.41-inch-diameter jet nozzle gave the lowest value of net-thrust specific fuel consumption.

  6. The 5-6 December 1991 FIRE IFO II jet stream cirrus case study: Possible influences of volcanic aerosols

    SciTech Connect

    Sassen, K.; Starr, D.O.C.; Melfi, S.H.; Spinhirne, J.D.; Poellot, M.R.; Eberhard, W.L.; Eloranta, E.W.; Hagen, D.E.; Hallett, J.

    1995-01-01

    In presenting an overview of the cirrus clouds comprehensively studied by ground-based and airborne sensors from Coffeyville, Kansas, during the 5-6 December 1992 Project FIRE IFO II case study period, evidence is provided that volcanic aerosols from the June 1991 Pinatubo eruptions may have significantly influenced the formation and maintenance of the cirrus. Following the local appearance of a spur of stratospheric volcanic debris from the subtropics, a series of jet streaks subsequently conditioned the troposphere through tropopause foldings with sulfur-based particles that became effective cloud-forming nuclei in cirrus clouds. Aerosol and ozone measurements suggest a complicated history of stratospheric-tropospheric exchanges embedded within the upper-level flow, and cirrus cloud formation was noted to occur locally at the boundaries of stratospheric aerosol-enriched layers that became humidified through diffusion, precipitation, or advective processes. Apparent cirrus cloud alterations include abnormally high ice crystal concentrations (up to {approximately}600 L{sup {minus}1}), complex radial ice crystal types, and relatively large haze particles in cirrus uncinus cell heads at temperatures between {minus}40{degrees} and {minus}50{degrees}C. Implications for volcanic-cirrus cloud climate effects and usual (nonvolcanic aerosol) jet stream cirrus cloud formation are discussed. 42 refs., 25 figs., 3 tabs.

  7. Probing the disc wind-jet connection in black hole transients II

    NASA Astrophysics Data System (ADS)

    Diaz Trigo, Maria

    2013-10-01

    We propose six observations of one high inclination black hole low mass X-ray binary (LMXB) at different stages of its outburst. We will investigate the presence of X-ray narrow absorption/emission features in the XMM spectra, which are a signature of a disc wind, and their relation to the accreting regime. Such features, identified with ions like FeXXV and FeXXVI, have been observed in a number of LMXBs and give us information about the mass outflow rate and the launching mechanism of the wind. With simultaneous radio observations we will probe the jet power as a function of the wind properties and how the radio flux density correlates with the X-ray flux at different accretion regimes. We will also investigate the broadening mechanism of the FeK emission line detected up to now in several LMXBs.

  8. Measurement of the top-quark mass in the lepton+jets channel using a matrix element technique with the CDF II detector

    DOE PAGESBeta

    Aaltonen, T.

    2011-10-14

    A measurement of the top-quark mass is presented using Tevatron data from proton-antiproton collisions at center-of-mass energy √s = 1.96 TeV collected with the CDF II detector. Events are selected from a sample of candidates for production of tt̄ pairs that decay into the lepton+jets channel. The top-quark mass is measured with an unbinned maximum likelihood method where the event probability density functions are calculated using signal and background matrix elements, as well as a set of parameterized jet-to-parton transfer functions. The likelihood function is maximized with respect to the top-quark mass, the signal fraction in the sample, and amore » correction to the jet energy scale (JES) calibration of the calorimeter jets. The simultaneous measurement of the JES correction ({Delta}{sub JES}) amounts to an additional in situ jet energy calibration based on the known mass of the hadronically decaying W boson. Using the data sample of 578 lepton+jets candidate events, corresponding to 3.2 fb-1 of integrated luminosity, the top-quark mass is measured to be mt = 172.4± 1.4 (stat + ΔJES) ± 1.3 (syst) GeV/c2.« less

  9. Measurement of the top-quark mass in the lepton+jets channel using a matrix element technique with the CDF II detector

    SciTech Connect

    Aaltonen, T.

    2011-10-14

    A measurement of the top-quark mass is presented using Tevatron data from proton-antiproton collisions at center-of-mass energy √s = 1.96 TeV collected with the CDF II detector. Events are selected from a sample of candidates for production of tt̄ pairs that decay into the lepton+jets channel. The top-quark mass is measured with an unbinned maximum likelihood method where the event probability density functions are calculated using signal and background matrix elements, as well as a set of parameterized jet-to-parton transfer functions. The likelihood function is maximized with respect to the top-quark mass, the signal fraction in the sample, and a correction to the jet energy scale (JES) calibration of the calorimeter jets. The simultaneous measurement of the JES correction ({Delta}{sub JES}) amounts to an additional in situ jet energy calibration based on the known mass of the hadronically decaying W boson. Using the data sample of 578 lepton+jets candidate events, corresponding to 3.2 fb-1 of integrated luminosity, the top-quark mass is measured to be mt = 172.4± 1.4 (stat + ΔJES) ± 1.3 (syst) GeV/c2.

  10. DICHOTOMY OF SOLAR CORONAL JETS: STANDARD JETS AND BLOWOUT JETS

    SciTech Connect

    Moore, Ronald L.; Cirtain, Jonathan W.; Sterling, Alphonse C.; Falconer, David A.

    2010-09-01

    By examining many X-ray jets in Hinode/X-Ray Telescope coronal X-ray movies of the polar coronal holes, we found that there is a dichotomy of polar X-ray jets. About two thirds fit the standard reconnection picture for coronal jets, and about one third are another type. We present observations indicating that the non-standard jets are counterparts of erupting-loop H{alpha} macrospicules, jets in which the jet-base magnetic arch undergoes a miniature version of the blowout eruptions that produce major coronal mass ejections. From the coronal X-ray movies we present in detail two typical standard X-ray jets and two typical blowout X-ray jets that were also caught in He II 304 A snapshots from STEREO/EUVI. The distinguishing features of blowout X-ray jets are (1) X-ray brightening inside the base arch in addition to the outside bright point that standard jets have, (2) blowout eruption of the base arch's core field, often carrying a filament of cool (T {approx} 10{sup 4} - 10{sup 5} K) plasma, and (3) an extra jet-spire strand rooted close to the bright point. We present cartoons showing how reconnection during blowout eruption of the base arch could produce the observed features of blowout X-ray jets. We infer that (1) the standard-jet/blowout-jet dichotomy of coronal jets results from the dichotomy of base arches that do not have and base arches that do have enough shear and twist to erupt open, and (2) there is a large class of spicules that are standard jets and a comparably large class of spicules that are blowout jets.

  11. Dichotomy of Solar Coronal Jets: Standard Jets and Blowout Jets

    NASA Technical Reports Server (NTRS)

    Moore, R. L.; Cirtain, J. W.; Sterling, A. C.; Falconer, D. A.

    2010-01-01

    By examining many X-ray jets in Hinode/XRT coronal X-ray movies of the polar coronal holes, we found that there is a dichotomy of polar X-ray jets. About two thirds fit the standard reconnection picture for coronal jets, and about one third are another type. We present observations indicating that the non-standard jets are counterparts of erupting-loop H alpha macrospicules, jets in which the jet-base magnetic arch undergoes a miniature version of the blowout eruptions that produce major CMEs. From the coronal X-ray movies we present in detail two typical standard X-ray jets and two typical blowout X-ray jets that were also caught in He II 304 Angstrom snapshots from STEREO/EUVI. The distinguishing features of blowout X-ray jets are (1) X-ray brightening inside the base arch in addition to the outside bright point that standard jets have, (2) blowout eruption of the base arch's core field, often carrying a filament of cool (T 10(exp 4) - 10(exp 5) K) plasma, and (3) an extra jet-spire strand rooted close to the bright point. We present cartoons showing how reconnection during blowout eruption of the base arch could produce the observed features of blowout X-ray jets. We infer that (1) the standard-jet/blowout-jet dichotomy of coronal jets results from the dichotomy of base arches that do not have and base arches that do have enough shear and twist to erupt open, and (2) there is a large class of spicules that are standard jets and a comparably large class of spicules that are blowout jets.

  12. Discovery of MK-7655, a β-lactamase inhibitor for combination with Primaxin®.

    PubMed

    Blizzard, Timothy A; Chen, Helen; Kim, Seongkon; Wu, Jane; Bodner, Rena; Gude, Candido; Imbriglio, Jason; Young, Katherine; Park, Young-Whan; Ogawa, Aimie; Raghoobar, Susan; Hairston, Nichelle; Painter, Ronald E; Wisniewski, Doug; Scapin, Giovanna; Fitzgerald, Paula; Sharma, Nandini; Lu, Jun; Ha, Sookhee; Hermes, Jeff; Hammond, Milton L

    2014-02-01

    β-Lactamase inhibitors with a bicyclic urea core and a variety of heterocyclic side chains were prepared and evaluated as potential partners for combination with imipenem to overcome class A and C β-lactamase mediated antibiotic resistance. The piperidine analog 3 (MK-7655) inhibited both class A and C β-lactamases in vitro. It effectively restored imipenem's activity against imipenem-resistant Pseudomonas and Klebsiella strains at clinically achievable concentrations. A combination of MK-7655 and Primaxin® is currently in phase II clinical trials for the treatment of Gram-negative bacterial infections. PMID:24433862

  13. L-H power threshold studies in JET with Be/W and C wall

    NASA Astrophysics Data System (ADS)

    Maggi, C. F.; Delabie, E.; Biewer, T. M.; Groth, M.; Hawkes, N. C.; Lehnen, M.; de la Luna, E.; McCormick, K.; Reux, C.; Rimini, F.; Solano, E. R.; Andrew, Y.; Bourdelle, C.; Bobkov, V.; Brix, M.; Calabro, G.; Czarnecka, A.; Flanagan, J.; Lerche, E.; Marsen, S.; Nunes, I.; Van Eester, D.; Stamp, M. F.; EFDA Contributors, JET

    2014-02-01

    A comparison of the L-H power threshold (Pthr) in JET with all carbon, JET-C, and beryllium/tungsten wall (the ITER-like choice), JET-ILW, has been carried out in experiments with slow input power ramps and matched plasma shapes, divertor configuration and IP/BT pairs. The low density dependence of the L-H power threshold, namely an increase below a minimum density ne,min, which was first observed in JET with the MkII-GB divertor and C wall and subsequently not observed with the current MkII-HD geometry, is observed again with JET-ILW. At plasma densities above ne,min, Pthr is reduced by ˜30%, and by ˜40% when the radiation from the bulk plasma is subtracted (Psep), with JET-ILW compared to JET-C. At the L-H transition the electron temperature at the edge, where the pedestal later develops, is also lower with JET-ILW, for a given edge density. With JET-ILW the minimum density is found to increase roughly linearly with magnetic field, n_{e,min} \\sim B_{T}^{4/5} , while the power threshold at the minimum density scales as P_{sep,\\min} \\sim B_{T}^{5/2} . The H-mode power threshold in JET-ILW is found to be sensitive both to variations in main plasma shape (Psep decreases with increasing lower triangularity and increases with upper triangularity) and in divertor configuration. When the data are recast in terms of Psep and Zeff or subdivertor neutral pressure a linear correlation is found, pointing to a possible role of Zeff and/or subdivertor neutral pressure in the L-H transition physics. Depending on the chosen divertor configuration, Pthr can be up to a factor of two lower than the ITPA scaling law for densities above ne,min. A shallow edge radial electric field well is observed at the L-H transition. The edge impurity ion poloidal velocity remains low, close to its L-mode values, ⩽5 km s-1 ± 2-3 km s-1, at the L-H transition and throughout the H-mode phase, with no measureable increase within the experimental uncertainties. The edge toroidal rotation

  14. [Fe II] 1.64 μm FEATURES OF JETS AND OUTFLOWS FROM YOUNG STELLAR OBJECTS IN THE CARINA NEBULA

    SciTech Connect

    Shinn, Jong-Ho; Lee, Jae-Joon; Chun, Moo-Young; Lyo, A.-Ran; Moon, Dae-Sik; Kyeong, Jaemann; Park, Byeong-Gon; Pyo, Tae-Soo; Lee, Ho-Gyu; Kim, Hyun-Jeong; Koo, Bon-Chul; Lee, Yong-Hyun; Sung, Hwankyung; Hur, Hyeonoh

    2013-11-01

    We present [Fe II] 1.64 μm imaging observations for jets and outflows from young stellar objects (YSOs) over the northern part (∼24' × 45') of the Carina Nebula, a massive star-forming region. The observations were performed with IRIS2 of the Anglo-Australian Telescope and the seeing was ∼1.''5 ± 0.''5. Eleven jet and outflow features are detected at eight different regions and are termed ionized Fe objects (IFOs). One Herbig-Haro candidate that was missed in Hubble Space Telescope Hα observations is newly identified as HHc-16, referring to our [Fe II] images. IFOs have knotty or longish shapes, and the detection rate of IFOs against previously identified YSOs is 1.4%, which should be treated as a lower limit. Four IFOs show anti-correlated peak intensities in [Fe II] and Hα, where the ratio I([Fe II])/I(Hα) is higher for longish IFOs than for knotty IFOs. We estimate the outflow mass loss rate from the [Fe II] flux using two different methods. The jet-driving objects are identified for three IFOs (IFO-2, -4, and -7) for which we study the relations between the outflow mass loss rate and the YSO physical parameters from the radiative transfer model fitting. The ratios of the outflow mass loss rate over the disk accretion rate for IFO-4 and -7 are consistent with the previously reported values (10{sup –2}-10{sup +1}), while the ratio is higher for IFO-2. This excess may result from underestimating the disk accretion rate. The jet-driving objects are likely to be low- or intermediate-mass stars. Other YSO physical parameters, such as luminosity and age, show reasonable relations or trends.

  15. Emerging jets

    NASA Astrophysics Data System (ADS)

    Schwaller, Pedro; Stolarski, Daniel; Weiler, Andreas

    2015-05-01

    In this work, we propose a novel search strategy for new physics at the LHC that utilizes calorimeter jets that (i) are composed dominantly of displaced tracks and (ii) have many different vertices within the jet cone. Such emerging jet signatures are smoking guns for models with a composite dark sector where a parton shower in the dark sector is followed by displaced decays of dark pions back to SM jets. No current LHC searches are sensitive to this type of phenomenology. We perform a detailed simulation for a benchmark signal with two regular and two emerging jets, and present and implement strategies to suppress QCD backgrounds by up to six orders of magnitude. At the 14 TeV LHC, this signature can be probed with mediator masses as large as 1.5 TeV for a range of dark pion lifetimes, and the reach is increased further at the high-luminosity LHC. The emerging jet search is also sensitive to a broad class of long-lived phenomena, and we show this for a supersymmetric model with R-parity violation. Possibilities for discovery at LHCb are also discussed.

  16. Particle diffusion and localized acceleration in inhomogeneous AGN jets - II. Stochastic variation

    NASA Astrophysics Data System (ADS)

    Chen, Xuhui; Pohl, Martin; Böttcher, Markus; Gao, Shan

    2016-05-01

    We study the stochastic variation of blazar emission under a 2D spatially resolved leptonic jet model we previously developed. Random events of particle acceleration and injection in small zones within the emission region are assumed to be responsible for flux variations. In addition to producing spectral energy distributions that describe the observed flux of Mrk 421, we further analyse the timing properties of the simulated light curves, such as the power spectral density (PSD) at different bands, flux-flux correlations, as well as the cross-correlation function between X-rays and TeV γ-rays. We find spectral breaks in the PSD at a time-scale comparable to the dominant characteristic time-scale in the system, which is usually the pre-defined decay time-scale of an acceleration event. Cooling imposes a delay, and so PSDs taken at lower energy bands in each emission component (synchrotron or inverse Compton) generally break at longer time-scales. The flux-flux correlation between X-rays and TeV γ-rays can be either quadratic or linear, depending on whether or not there are large variation of the injection into the particle acceleration process. When the relationship is quadratic, the TeV flares lag the X-ray flares, and the optical and GeV flares are large enough to be comparable to the ones in X-ray. When the relationship is linear, the lags are insignificant, and the optical and GeV flares are small.

  17. ADAPTIVE OPTICS ECHELLE SPECTROSCOPY OF [Fe II] 1.644 mum IN THE RW AUR JET: A NARROW SLICE DOWN THE AXIS OF THE FLOW

    SciTech Connect

    Hartigan, Patrick; Hillenbrand, Lynne

    2009-11-10

    We present new adaptive optics echelle spectra of the near-infrared [Fe II] lines in the redshifted and blueshifted jets from the T Tauri star RW Aur. The spectra have an unprecedented combination of high spatial and spectral resolution that makes it possible to trace the dynamics of the flow to a projected distance of only 10 AU from the source. As noted by previous studies, the redshifted flow is much slower than its fainter blueshifted counterpart. Our observations clearly show that both the radial velocities and the emission line widths are larger closer to the source on both sides of the jet. The line widths are 20%-30% of the jet velocity on both sides of the flow, significantly larger than would be produced by a divergent constant velocity flow. The observed line widths could arise from a layered velocity structure in the jet or from magnetic waves. A bright knot in the redshifted jet has no concomitant increase in line width, implying that it is not heated by a bow shock. Alternate heating mechanisms include planar shocks, ambipolar diffusion, and magnetic reconnection.

  18. Effect of the Angiotensin I Converting Enzyme Inhibitor, MK-421, on Experimentally Induced Drinking

    NASA Technical Reports Server (NTRS)

    Fregley, Melvin J.; Fater, Dennis C.; Greenleaf, John E.

    1982-01-01

    MK-421, the ethyl ester maleate salt of N-(S)-1-(ethoxycarbonyl)-3-phenyl-propyl- Ala-L-Pro, is an angiotensin I converting enzyme inhibitor. An initial objective was to determine whether MK-421, administered at 0, 2.5, 5.0, 10.0, 20.0 and 40.0 mg/kg, ip to 96 female rats 15 min prior to administration of the beta-adrenergic agonist, isoproterenol (25 microgram/kg, ip), would inhibit the drinking induced by isoproterenol during 2 h after its administration. The water intake induced by isoproterenol was inhibited significantly by 2.5 mg MK-421/kg. When a similar experiment was performed using Angiotensin I (AI) (200 microgram/kg, ip) as the dipsogenic agent, MK-421 (5 mg/kg, ip), administered 15 min prior to AI, inhibited significantly both the dipsogenic and the diuretic effect of AI. However, administration of angiotensin II (AII, 200 microgram/kg, ip) 15 min after MK-421 (5mg/kg) was accompanied by a water intake that did not differ from AII alone. The drink induced by ip administration of 1.0 m NaCl solution (1% of body wt, ip) was not inhibited by administration of MK-421 (5 mg/kg) 15 min prior to allowing access to water while the drink induced by a 24 h dehydration was partially inhibited. Thus, the drinks induced by administraition of either isoproterenol or AI are dependent on formation of AII. That induced by dehydration is partially dependent, while that induced by hypertonic siilinc is independent of the formation of AII.

  19. A magnetohydrodynamic model of the M87 jet. II. Self-consistent quad-shock jet model for optical relativistic motions and particle acceleration

    SciTech Connect

    Nakamura, Masanori

    2014-04-20

    We describe a new paradigm for understanding both relativistic motions and particle acceleration in the M87 jet: a magnetically dominated relativistic flow that naturally produces four relativistic magnetohydrodynamic (MHD) shocks (forward/reverse fast and slow modes). We apply this model to a set of optical super- and subluminal motions discovered by Biretta and coworkers with the Hubble Space Telescope during 1994-1998. The model concept consists of ejection of a single relativistic Poynting jet, which possesses a coherent helical (poloidal + toroidal) magnetic component, at the remarkably flaring point HST-1. We are able to reproduce quantitatively proper motions of components seen in the optical observations of HST-1 with the same model we used previously to describe similar features in radio very long baseline interferometry observations in 2005-2006. This indicates that the quad relativistic MHD shock model can be applied generally to recurring pairs of super/subluminal knots ejected from the upstream edge of the HST-1 complex as observed from radio to optical wavelengths, with forward/reverse fast-mode MHD shocks then responsible for observed moving features. Moreover, we identify such intrinsic properties as the shock compression ratio, degree of magnetization, and magnetic obliquity and show that they are suitable to mediate diffusive shock acceleration of relativistic particles via the first-order Fermi process. We suggest that relativistic MHD shocks in Poynting-flux-dominated helical jets may play a role in explaining observed emission and proper motions in many active galactic nuclei.

  20. Relativistic Jets in the Radio Reference Frame Image Database. II. Blazar Jet Accelerations from the First 10 Years of Data (1994-2003)

    NASA Astrophysics Data System (ADS)

    Piner, B. G.; Pushkarev, A. B.; Kovalev, Y. Y.; Marvin, C. J.; Arenson, J. G.; Charlot, P.; Fey, A. L.; Collioud, A.; Voitsik, P. A.

    2012-10-01

    We analyze blazar jet apparent speeds and accelerations from the RDV series of astrometric and geodetic very long baseline interferometry (VLBI) experiments. From these experiments, we have produced and analyzed 2753 global VLBI images of 68 sources at 8 GHz with a median beam size of 0.9 milliarcseconds (mas) and a median of 43 epochs per source. From this sample, we analyze the motions of 225 jet components in 66 sources. The distribution of the fastest measured apparent speed in each source has a median of 8.3c and a maximum of 44c. Sources in the 2FGL Fermi LAT catalog display higher apparent speeds than those that have not been detected. On average, components farther from the core in a given source have significantly higher apparent speeds than components closer to the core; for example, for a typical source, components at ~3 mas from the core (~15 pc projected at z ~ 0.5) have apparent speeds about 50% higher than those of components at ~1 mas from the core (~5 pc projected at z ~ 0.5). We measure accelerations of components in orthogonal directions parallel and perpendicular to their average velocity vector. Parallel accelerations have significantly larger magnitudes than perpendicular accelerations, implying that observed accelerations are predominantly due to changes in the Lorentz factor (bulk or pattern) rather than projection effects from jet bending. Positive parallel accelerations are significantly more common than negative ones, so the Lorentz factor (bulk or pattern) tends to increase on the scales observed here. Observed parallel accelerations correspond to modest source frame increases in the bulk or pattern Lorentz factor.

  1. SU2C Phase Ib Study of Paclitaxel and MK-2206 in Advanced Solid Tumors and Metastatic Breast Cancer

    PubMed Central

    Gonzalez-Angulo, Ana M.; Krop, Ian; Akcakanat, Argun; Chen, Huiqin; Liu, Shuying; Li, Yisheng; Culotta, Kirk S.; Tarco, Emily; Piha-Paul, Sarina; Moulder-Thompson, Stacy; Velez-Bravo, Vivianne; Sahin, Aysegul A.; Doyle, Laurence A.; Do, Kim-Anh; Winer, Eric P.; Mills, Gordon B.; Kurzrock, Razelle

    2015-01-01

    Background: There is preclinical synergism between taxanes and MK-2206. We aim to determine the maximum tolerated dose, safety, and activity of combining MK-2206 and paclitaxel in metastatic cancer. Methods: Patients received weekly doses of paclitaxel at 80mg/m2 on day 1, followed by MK-2206 orally on day 2 escalated at 90mg, 135mg, and 200mg. Treatment continued until progression, excessive toxicity, or patient request. Blood and tissue were collected for pharmacokinetic and pharmacodynamics markers. A cycle consisted of three weeks of therapy. Dose-limiting toxicity (DLT) was defined as unacceptable toxicity during the first cycle. All statistical tests were two-sided. Results: Twenty-two patients were treated, nine in dose escalation and 13 in dose expansion. Median age was 55 years. Median number of cycles was four. Dose escalation was completed with no DLT. CTCAE Grade 3 or higher adverse events were fatigue (n = 2), rash (n = 2), hyperglycemia (n = 1), and neutropenia (n = 7). Four patients in the expansion phase required MK-2206 dose reduction. Phase II recommended dose was established as paclitaxel 80mg/m2 weekly on day 1, and MK-2206 135mg weekly on day 2. Paclitaxel systemic exposure was similar in the presence or absence of MK-2206. Plasma MK-2206 concentrations were similar to data from previous phase I monotherapy. There was a statistically significant decrease in expression of pAKT S473 (P = .01) and pAKT T308 (P = .002) after therapy. PI3K/AKT/mTOR downregulation in tumor tissues and circulating markers did not correlate with tumor response or clinical benefit. There were five objective responses, and nine patients had stable disease. Conclusion: MK-2206 was well tolerated with paclitaxel. Preliminary antitumor activity was documented. PMID:25688104

  2. In vitro cytokine release from rat type II pneumocytes and alveolar macrophages following exposure to JP-8 jet fuel in co-culture.

    PubMed

    Wang, Shengjun; Young, R Scotte; Sun, Nina N; Witten, Mark L

    2002-05-01

    Alveolar type II epithelial cells (AIIE) and pulmonary alveolar macrophages (PAM) are involved in pulmonary toxicity of JP-8 jet fuel exposure. To further elucidate their inflammatory mechanisms, the effect(s) of JP-8 jet fuel on cytokine secretion were examined in a transformed rat AIIE cell line (RLE-6TN) culture alone, primary PAM (from Fischer 344 rats) culture alone, and the co-culture of AIIE and primary PAM. A series of JP-8 jet fuel concentrations (0-0.8 microg/ml), which may actually be encountered in alveolar space of lungs exposed in vivo, were placed in cell culture for 24 h. Cultured AIIE alone secreted spontaneously interleukin (IL)-1beta and -6 [below detectable limits for IL-10 and tumor necrosis factor-alpha (TNF-alpha)], whereas cultured PAM alone secreted IL-1beta, -10, and TNF-alpha, in a concentration-dependent manner. These data suggest that the release of cytokines, not only from PAM but also from AIIE cells, may contribute to JP-8 jet fuel-induced inflammatory response in the alveolar space. However, the co-cultures of AIIE and PAM showed no significant changes in IL-1beta, -6, and TNF-alpha at any JP-8 jet fuel concentration compared to control values. These cytokine levels in co-cultures of AIIE and PAM were inversely related to these of cultured AIIE or PAM alone. Interestingly, IL-10 levels in the co-culture system were concentration-dependently increased up to 1058% at JP-8 concentrations of 0.8 microg/ml, although under detectable limits in cultured AIIE alone and no significant concentration change in cultured PAM alone. It appears that PAM may possibly act via paracrine and/or autocrine pathways to signal AIIE cells to regulate cytokine release. PMID:11960674

  3. [Fe II] 1.257 μm and He I 1.083 μm Emission in the Central Region of the Orion Nebula: H II Region, HH Flows, Jets, and Proplyds

    NASA Astrophysics Data System (ADS)

    Takami, Michihiro; Usuda, Tomonori; Sugai, Hajime; Suto, Hiroshi; Pyo, Tae-Soo; Takeyama, Norihide; Aoki, Tetsuo; Mizutani, Kohei; Tanaka, Masuo

    2002-02-01

    The [Fe II] 1.257 μm and He I 1.083 μm emission lines were observed in the central 6'×8' region of the Orion Nebula, and their excitation in the photoionized H II region, HH flows, jets, and proplyds is investigated. Observations were carried out using the imaging Fabry-Perot spectrometer MUSE at the National Astronomical Observatory of Japan 1.5 m infrared telescope, which provides a 4'×4' field of view and a spectral resolution λ/δλ of ~2000 at the observed wavelengths. The [Fe II] images exhibit (1) filamentary structures and diffuse emission, which presumably arise from ionization fronts of the photoionized H II region, and (2) a number of knots, some of which are newly identified. Centroidal velocities in most of the knots are negative relative to those in the ionization fronts by up to -60 km s-1, and observed line profiles in the bright knots exhibit blueshifted wings, agreeing with bow shock models. The He I 1.083 μm emission in the observed region is dominated by the photoionized H II region, and its distribution reflects the complicated nature of the excitation. The He I images also contain blueshifted emission from several HH flows and jets and redshifted emission associated with proplyds. Our results for the shocks suggest that the [Fe II] 1.257 μm and He I 1.083 μm emission reflects the ionization of the preshock gas: the [Fe II] 1.257 μm emission is prominent in shocks propagating in molecular/atomic gas, while the He I 1.083 μm is prominent in shocks in the photoionized H II region. Different line excitation in these shocks can be explained by the following physical properties and processes: (1) difference of the excitation energies from the ground state (1 and 20 eV for the [Fe II] and He I lines, respectively), (2) resonance scattering and collisional excitation from the metastable state, enhancing the He I 1.083 μm line in shocks in the photoionized H II region, (3) a large photoionization cross section of Fe+, causing the absence of

  4. MK2 Regulates Ras Oncogenesis through Stimulating ROS Production

    PubMed Central

    Kobayashi, Yusuke; Qi, Xiaomei

    2012-01-01

    Ras signals through both mitogenic and stress pathways and studies of Ras regulatory effects of stress pathways hold great promise to control Ras-dependent malignancies. Our previous work showed Ras activation of a stress kinase (MAPK-activated protein kinase 2 [MK2]), and here, we examine regulatory effects of MK2 on Ras oncogenesis. MK2 knockout was shown to increase Ras transformation in mouse embryonic fibroblasts (MEFs) in vitro and to enhance the resultant tumor growth in mice, indicating a tumor suppressor activity. In Ras-dependent and -independent human colon cancer, however, MK2-forced expression increases and MK2 depletion decreases the malignant growth, suggesting its oncogenic activity. The oncogenic activity of MK2 couples with its activation by both stress and mitogenic signals through extracellular signal–regulated kinase and p38α pathways, whereas its tumor-suppressing effect links to its stimulation only by stress downstream of p38α. Of interest, MK2 was shown to decrease intracellular levels of reactive oxygen species (ROS) in MEFs but increase its production in human colon cancer cells, and experiments with antioxidants revealed that ROS is required for Ras oncogenesis in both systems. These results indicate that MK2 can increase or decrease Ras oncogenesis dependent of its ROS regulatory activities. PMID:23264852

  5. ACS Chemical Neuroscience Molecule Spotlight on Telcagepant (MK-0974)

    PubMed Central

    2011-01-01

    Telcagepant (MK-0974) is a novel calcitonin gene-related peptide (CGRP) receptor antagonist currently undergoing clinical trials for migraine (http://www.merck.com/research/pipeline/home.html). MK-0974 is currently being studied in phase III clinical trials. PMID:22816019

  6. Measurement of the Inclusive Jet Cross Section using the k(T) algorithm in p anti-p collisions at s**(1/2) = 1.96-TeV with the CDF II Detector

    SciTech Connect

    Abulencia, A.; Adelman, J.; Affolder, Anthony Allen; Akimoto, T.; Albrow, Michael G.; Ambrose, D.; Amerio, S.; Amidei, Dante E.; Anastassov, A.; Anikeev, Konstantin; Annovi, A.; /Frascati /Comenius U.

    2007-01-01

    The authors report on measurements of the inclusive jet production cross section as a function of the jet transverse momentum in p{bar p} collisions at {radical}s = 1.96 TeV, using the k{sub T} algorithm and a data sample corresponding to 1.0 fb{sup -1} collected with the Collider Detector at Fermilab in Run II. The measurements are carried out in five different jet rapidity regions with |y{sup jet}| < 2.1 and transverse momentum in the range 54 < p{sub T}{sup jet} < 700 GeV/c. Next-to-leading order perturbative QCD predictions are in good agreement with the measured cross sections.

  7. Search for Anomalous Production of Photon + Jets + Missing Transverse Energy in $p\\bar{p}$ Collisions at $\\sqrt{s}=1.96$~TeV Using the CDF II Detector

    SciTech Connect

    Hewamanage, Samantha Kaushalya

    2011-01-01

    A model-independent signature-based search for physics beyond the Standard Model is performed in the photon~+ jets~+ missing transverse energy channel in \\ppbar collisions at a center of mass energy of 1.96 TeV using the CDF II detector. Events with a photon + jets are predicted by the Standard Model and also by many theoretical models beyond the Standard Model. In the Standard Model, the main mechanisms for photon~+ jets production include quark-antiquark annihilation and quark-gluon scattering. No intrinsic missing transverse energy is present in any of these Standard Model processes. In this search, photon~+ $\\geq$1 jet and photon~+ $\\geq$2 jet events are analyzed with and without a minimum requirement on the missing transverse energy. Numerous mass distributions and kinematic distributions are studied and no significant excess over the background prediction is found. All results indicate good agreement with expectations of the Standard Model.

  8. A DEEP CHANDRA ACIS STUDY OF NGC 4151. II. THE INNERMOST EMISSION LINE REGION AND STRONG EVIDENCE FOR RADIO JET-NLR CLOUD COLLISION

    SciTech Connect

    Wang Junfeng; Fabbiano, Giuseppina; Elvis, Martin; Risaliti, Guido; Karovska, Margarita; Zezas, Andreas; Mundell, Carole G.

    2011-07-20

    We have studied the X-ray emission within the inner {approx}150 pc radius of NGC 4151 by constructing high spatial resolution emission line images of blended O VII, O VIII, and Ne IX. These maps show extended structures that are spatially correlated with the radio outflow and optical [O III] emission. We find strong evidence for jet-gas cloud interaction, including morphological correspondences with regions of X-ray enhancement, peaks of near-infrared [Fe II] emission, and optical clouds. In these regions, moreover, we find evidence of elevated Ne IX/O VII ratios; the X-ray emission of these regions also exceeds that expected from nuclear photoionization. Spectral fitting reveals the presence of a collisionally ionized component. The thermal energy of the hot gas suggests that {approx}> 0.1% of the estimated jet power is deposited into the host interstellar medium through interaction between the radio jet and the dense medium of the circumnuclear region. We find possible pressure equilibrium between the collisionally ionized hot gas and the photoionized line-emitting cool clouds. We also obtain constraints on the extended iron and silicon fluorescent emission. Both lines are spatially unresolved. The upper limit on the contribution of an extended emission region to the Fe K{alpha} emission is {approx}< 5% of the total, in disagreement with a previous claim that 65% of the Fe K{alpha} emission originates in the extended narrow line region.

  9. Pharmacokinetics and food interaction of MK-462 in healthy males.

    PubMed

    Cheng, H; Polvino, W J; Sciberras, D; Yogendran, L; Cerchio, K A; Christie, K; Olah, T V; McLoughlin, D; James, I; Rogers, J D

    1996-01-01

    A study was conducted to assess the safety, tolerability, and pharmacokinetics of single intravenous (IV) doses of 5-90 micrograms kg-1 of MK-462, and the effect of food on the pharmacokinetics of MK-462 administered orally to healthy males. Results of this study indicate that IV doses of MK-462 from 5 to 90 micrograms kg-1 are well tolerated. The disposition kinetics of MK-462 were linear for IV doses up to and including 60 micrograms kg-1. The values of the plasma clearance (CL), steady-state volume of distribution (Vss), plasma terminal half-life (t1/2), and mean residence time in the body (MRT) of MK-462 averaged 1376 mL min-1, 140 L, 1.8 h, and 1.7 h, respectively, and remained essentially constant over the dosage range of 10-60 micrograms kg-1 of IV MK-462. However, as the dose increased from 60 to 90 micrograms kg-1, the mean value of the apparent CL decreased from 1376 to 807 mL min-1. Thus, elimination of MK-462 was dose dependent in this dosage range. Based on the disposition decomposition analysis (DDA), it was shown that the Vss value of MK-462 remained essentially constant over the dosage range of 10-90 micrograms kg-1 of IV MK-462. The following values of two dose-independent parameters were also calculated by using DDA: distribution clearance (CLd) = 2028 mL min-1, and mean transit time in the peripheral tissues (MTTT) = 0.74 h. The mean values of AUC, Cmax, tmax, and apparent t1/2 of MK-462 in 12 subjects each receiving a 40 mg tablet of MK-462 without breakfast were 330 ng.h mL-1, 77 ng mL-1, 1.6 h, and 1.8 h, respectively. Although administration of a standard breakfast prior to dosing increased the AUC value (by approximately 20%) of MK-462 and delayed its absorption, there were no significant effects of the meal on the values of Cmax and apparent t1/2 of MK-462. PMID:8991488

  10. In vitro time- and dose-effect response of JP-8 and S-8 jet fuel on alveolar type II epithelial cells of rats.

    PubMed

    Robb, Tiffany M; Rogers, Michael J; Woodward, Suann S; Wong, Simon S; Witten, Mark L

    2010-07-01

    This study was designed to characterize and compare the effects of jet propellant-8 (JP-8) fuel and synthetic-8 (S-8) on cell viability and nitric oxide synthesis in cultured alveolar type II epithelial cells of rats. Exposure times varied from 0.25, 0.5, 1, and 6 hours at the following concentrations of jet fuel: 0.0, 0.1, 0.4, and 2.0 microg/mL. Data indicate that JP-8 presents a gradual decline in cell viability and steady elevation in nitric oxide release as exposure concentrations increase. At a 2.0 microg/mL concentration of JP-8, nearly all of the cells are not viable. Moreover, S-8 exposure to rat type II lung cells demonstrated an abrupt fall in percentage cell viability and increases in nitric oxide measurement, particularly after the 2.0 microg/mL was reached at 1 and 6 hours. At 0.0, 0.2, and 0.4 microg/mL concentrations of S-8, percentage viability was sustained at steady concentrations. The results suggest different epithelial toxicity and mechanistic effects of S-8 and JP-8, providing further insight concerning the impairment imposed at specific levels of lung function and pathology induced by the different fuels. PMID:20504826

  11. Complete multiwavelength evolution of galactic black hole transients during outburst decay. II. Compact jets and X-ray variability properties

    SciTech Connect

    Dinçer, T.; Kalemci, E.; Tomsick, J. A.; Buxton, M. M.; Bailyn, C. D.

    2014-11-01

    We investigated the relation between compact jet emission and X-ray variability properties of all black hole transients with multiwavelength coverage during their outburst decays. We studied the evolution of all power spectral components (including low-frequency quasi-periodic oscillations; QPOs), and related this evolution to changes in jet properties tracked by radio and infrared observations. We grouped sources according to their tracks in the radio/X-ray luminosity relation and show that the standards show stronger broadband X-ray variability than outliers at a given X-ray luminosity when the compact jet turns on. This trend is consistent with the internal shock model and can be important for the understanding of the presence of tracks in the radio/X-ray luminosity relation. We also observed that the total and the QPO rms amplitudes increase together during the earlier part of the outburst decay, but after the compact jet turns, either the QPO disappears or its rms amplitude decreases significantly while the total rms amplitudes remain high. We discuss these results with a scenario including a variable corona and a non-variable disk with a mechanism for the QPO separate from the mechanism that creates broad components. Finally, we evaluated the timing predictions of the magnetically dominated accretion flow model that can explain the presence of tracks in the radio/X-ray luminosity relation.

  12. Twin Jet

    NASA Technical Reports Server (NTRS)

    Henderson, Brenda; Bozak, Rick

    2010-01-01

    Many subsonic and supersonic vehicles in the current fleet have multiple engines mounted near one another. Some future vehicle concepts may use innovative propulsion systems such as distributed propulsion which will result in multiple jets mounted in close proximity. Engine configurations with multiple jets have the ability to exploit jet-by-jet shielding which may significantly reduce noise. Jet-by-jet shielding is the ability of one jet to shield noise that is emitted by another jet. The sensitivity of jet-by-jet shielding to jet spacing and simulated flight stream Mach number are not well understood. The current experiment investigates the impact of jet spacing, jet operating condition, and flight stream Mach number on the noise radiated from subsonic and supersonic twin jets.

  13. Shock associated noise of inverted-profile coannular jets. II - Condition for minimum noise. III - Shock structure and noise characteristic

    NASA Technical Reports Server (NTRS)

    Tam, C. K. W.; Tanna, H. K.

    1985-01-01

    The generation of noise by the shock-turbulence interaction with shock cells in an inverted-profile coannular jet with nozzle exit velocity aligned with the jet axis is investigated analytically, interpreting the optical measurements of Tanna et al. (1985). The noise-intensity minimum at slightly supersonic primary-flow velocities is related to the weakness of the primary-stream shock-cell structure and the lack of such a pattern in the outer fan stream. The discrepancies between this finding and those of Dosanjh et al. (1977 and 1978) are attributed to nozzle design, the definition of minimum noise, and different interpretative approaches. A first-order shock-cell model is then developed to derive formulas for the peak frequencies and the scaling of noise intensity. The results of computations using these formulas are presented in graphs and found to be in good agreement with the experimental data.

  14. THE FERMI BUBBLES. II. THE POTENTIAL ROLES OF VISCOSITY AND COSMIC-RAY DIFFUSION IN JET MODELS

    SciTech Connect

    Guo Fulai; Mathews, William G.; Oh, S. Peng

    2012-09-10

    The origin of the Fermi bubbles recently detected by the Fermi Gamma-ray Space Telescope in the inner Galaxy is mysterious. In the companion paper Guo and Mathews (Paper I), we use hydrodynamic simulations to show that they could be produced by a recent powerful active galactic nucleus (AGN) jet event. Here, we further explore this scenario to study the potential roles of shear viscosity and cosmic-ray (CR) diffusion on the morphology and CR distribution of the bubbles. We show that even a relatively low level of viscosity ({mu}{sub visc} {approx}> 3 g cm{sup -1} s{sup -1}, or {approx}0.1%-1% of Braginskii viscosity in this context) could effectively suppress the development of Kelvin-Helmholtz instabilities at the bubble surface, resulting in smooth bubble edges as observed. Furthermore, viscosity reduces circulating motions within the bubbles, which would otherwise mix the CR-carrying jet backflow near bubble edges with the bubble interior. Thus viscosity naturally produces an edge-favored CR distribution, an important ingredient to produce the observed flat gamma-ray surface brightness distribution. Generically, such a CR distribution often produces a limb-brightened gamma-ray intensity distribution. However, we show that by incorporating CR diffusion that is strongly suppressed across the bubble surface (as inferred from sharp bubble edges) but is close to canonical values in the bubble interior, we obtain a reasonably flat gamma-ray intensity profile. The similarity of the resulting CR bubble with the observed Fermi bubbles strengthens our previous result in Paper I that the Fermi bubbles were produced by a recent AGN jet event. Studies of the nearby Fermi bubbles may provide a unique opportunity to study the potential roles of plasma viscosity and CR diffusion on the evolution of AGN jets and bubbles.

  15. Nanoelectronic primary thermometry below 4 mK

    NASA Astrophysics Data System (ADS)

    Bradley, D. I.; George, R. E.; Gunnarsson, D.; Haley, R. P.; Heikkinen, H.; Pashkin, Yu. A.; Penttilä, J.; Prance, J. R.; Prunnila, M.; Roschier, L.; Sarsby, M.

    2016-01-01

    Cooling nanoelectronic structures to millikelvin temperatures presents extreme challenges in maintaining thermal contact between the electrons in the device and an external cold bath. It is typically found that when nanoscale devices are cooled to ~10 mK the electrons are significantly overheated. Here we report the cooling of electrons in nanoelectronic Coulomb blockade thermometers below 4 mK. The low operating temperature is attributed to an optimized design that incorporates cooling fins with a high electron-phonon coupling and on-chip electronic filters, combined with low-noise electronic measurements. By immersing a Coulomb blockade thermometer in the 3He/4He refrigerant of a dilution refrigerator, we measure a lowest electron temperature of 3.7 mK and a trend to a saturated electron temperature approaching 3 mK. This work demonstrates how nanoelectronic samples can be cooled further into the low-millikelvin range.

  16. Nanoelectronic primary thermometry below 4 mK.

    PubMed

    Bradley, D I; George, R E; Gunnarsson, D; Haley, R P; Heikkinen, H; Pashkin, Yu A; Penttilä, J; Prance, J R; Prunnila, M; Roschier, L; Sarsby, M

    2016-01-01

    Cooling nanoelectronic structures to millikelvin temperatures presents extreme challenges in maintaining thermal contact between the electrons in the device and an external cold bath. It is typically found that when nanoscale devices are cooled to ∼ 10 mK the electrons are significantly overheated. Here we report the cooling of electrons in nanoelectronic Coulomb blockade thermometers below 4 mK. The low operating temperature is attributed to an optimized design that incorporates cooling fins with a high electron-phonon coupling and on-chip electronic filters, combined with low-noise electronic measurements. By immersing a Coulomb blockade thermometer in the (3)He/(4)He refrigerant of a dilution refrigerator, we measure a lowest electron temperature of 3.7 mK and a trend to a saturated electron temperature approaching 3 mK. This work demonstrates how nanoelectronic samples can be cooled further into the low-millikelvin range. PMID:26816217

  17. Mk12A/W78 ground test project (u)

    SciTech Connect

    Stokes, Kyle R

    2010-12-01

    The slides present the scope, objectives and status of the Mk12A1W78 Ground Test Project for the purpose of updating the ICBM Project Officers Group. In addition, project constraints and risks are discussed.

  18. Jet shielding of jet noise

    NASA Technical Reports Server (NTRS)

    Simonich, J. C.; Amiet, R. K.; Schlinker, R. H.

    1986-01-01

    An experimental and theoretical study was conducted to develop a validated first principle analysis for predicting the jet noise reduction achieved by shielding one jet exhaust flow with a second, closely spaced, identical jet flow. A generalized fuel jet noise analytical model was formulated in which the acoustic radiation from a source jet propagates through the velocity and temperature discontinuity of the adjacent shielding jet. Input variables to the prediction procedure include jet Mach number, spacing, temperature, diameter, and source frequency. Refraction, diffraction, and reflection effects, which control the dual jet directivity pattern, are incorporated in the theory. The analysis calculates the difference in sound pressure level between the dual jet configuration and the radiation field based on superimposing two independent jet noise directivity patterns. Jet shielding was found experimentally to reduce noise levels in the common plane of the dual jet system relative to the noise generated by two independent jets.

  19. Impulsive Heating of Solar Flare Ribbons Above 10 MK

    NASA Astrophysics Data System (ADS)

    Simões, P. J. A.; Graham, D. R.; Fletcher, L.

    2015-12-01

    The chromospheric response to the input of flare energy is marked by extended extreme ultraviolet (EUV) ribbons and hard X-ray (HXR) footpoints. These are usually explained as the result of heating and bremsstrahlung emission from accelerated electrons colliding in the dense chromospheric plasma. We present evidence of impulsive heating of flare ribbons above 10 MK in a two-ribbon flare. We analyse the impulsive phase of SOL2013-11-09T06:38, a C2.6 class event using data from Atmospheric Imaging Assembly (AIA) on board of the Solar Dynamics Observatory (SDO) and the Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI) to derive the temperature, emission measure and differential emission measure of the flaring regions and investigate the evolution of the plasma in the flaring ribbons. The ribbons were visible at all SDO/AIA EUV/UV wavelengths, in particular, at 94 and 131 Å filters, sensitive to temperatures of 8 MK and 12 MK. The time evolution of the emission measure of the plasma above 10 MK at the ribbons has a peak near the HXR peak time. The presence of hot plasma in the lower atmosphere is further confirmed by a RHESSI imaging spectroscopy analysis, which shows resolved sources at 11 - 13 MK that are associated with at least one ribbon. We found that collisional beam-heating can only marginally explain the power necessary to heat the 10 MK plasma at the ribbons.

  20. Interrogating two schedules of the AKT inhibitor MK-2206 in patients with advanced solid tumors incorporating novel pharmacodynamic and functional imaging biomarkers

    PubMed Central

    Yap, Timothy A.; Yan, Li; Patnaik, Amita; Tunariu, Nina; Biondo, Andrea; Fearen, Ivy; Papadopoulos, Kyriakos P.; Olmos, David; Baird, Richard; Delgado, Liliana; Tetteh, Ernestina; Beckman, Robert A.; Lupinacci, Lisa; Riisnaes, Ruth; Decordova, Shaun; Heaton, Simon P.; Swales, Karen; deSouza, Nandita M; Leach, Martin O.; Garrett, Michelle D.; Sullivan, Daniel M.; de Bono, Johann S.; Tolcher, Anthony W.

    2014-01-01

    Purpose Multiple cancers harbor genetic aberrations that impact AKT signaling. MK-2206 is a potent pan-AKT inhibitor with a maximum tolerated dose (MTD) previously established at 60mg on alternate days (QOD). Due to a long half-life (60-80h), a weekly (QW) MK-2206 schedule was pursued to compare intermittent QW and continuous QOD dosing. Experimental Design Patients with advanced cancers were enrolled onto a QW dose-escalation phase I study to investigate the safety and pharmacokinetic-pharmacodynamic profiles of tumor and platelet-rich plasma (PRP). The QOD MTD of MK-2206 was also assessed in patients with ovarian and castration-resistant prostate cancers, and patients with advanced cancers undergoing multiparametric functional magnetic resonance imaging (MRI) studies, including dynamic contrast-enhanced MRI, diffusion-weighted imaging, magnetic resonance spectroscopy and intrinsic susceptibility-weighted MRI. Results Seventy-one patients were enrolled; 38 patients had 60mg MK-2206 QOD, while 33 received MK-2206 at 90mg, 135mg, 150mg, 200mg, 250mg, and 300mg QW. The QW MK-2206 MTD was established at 200mg following dose-limiting rash at 250mg and 300mg. QW dosing appeared to be similarly tolerated to QOD, with toxicities including rash, gastrointestinal symptoms, fatigue, and hyperglycemia. Significant AKT pathway blockade was observed with both continuous QOD and intermittent QW dosing of MK-2206 in serially-obtained tumor and PRP specimens. The functional imaging studies demonstrated that complex multiparametric MRI protocols may be effectively implemented in a phase I trial. Conclusions MK-2206 safely results in significant AKT pathway blockade in QOD and QW schedules. The intermittent dose of 200mg QW is currently used in phase II MK-2206 monotherapy and combination studies. PMID:25239610

  1. The impact of dynamic data assimilation on the numerical simulations of the QE II cyclone and an analysis of the jet streak influencing the precyclogenetic environment

    NASA Technical Reports Server (NTRS)

    Manobianco, John; Uccellini, Louis W.; Brill, Keith F.; Kuo, Ying-Hwa

    1992-01-01

    A mesoscale numerical model is combined with a dynamic data assimilation via Newtonian relaxation, or 'nudging', to provide initial conditions for subsequent simulations of the QE II cyclone. Both the nudging technique and the inclusion of supplementary data are shown to have a large positive impact on the simulation of the QE II cyclone during the initial phase of rapid cyclone development. Within the initial development period (from 1200 to 1800 UTC 9 September 1978), the dynamic assimilation of operational and bogus data yields a coherent two-layer divergence pattern that is not well defined in the model run using only the operational data and static initialization. Diagnostic analysis based on the simulations show that the initial development of the QE II storm between 0000 UTC 9 September and 0000 UTC 10 September was embedded within an indirect circulation of an intense 300-hPa jet streak, was related to baroclinic processes extending throughout a deep portion of the troposphere, and was associated with a classic two-layer mass-divergence profile expected for an extratropical cyclone.

  2. Critical evaluation of Jet-A spray combustion using propane chemical kinetics in gas turbine combustion simulated by KIVA-II

    NASA Technical Reports Server (NTRS)

    Nguyen, H. L.; Ying, S.-J.

    1990-01-01

    Numerical solutions of the Jet-A spray combustion were obtained by means of the KIVA-II computer code after Jet-A properties were added to the 12 chemical species the program had initially contained. Three different reaction mechanism models are considered. The first model consists of 131 reactions and 45 species; it is evaluated by comparing calculated ignition delay times with available shock tube data, and it is used in the evaluation of the other two simplified models. The simplified mechanisms consider 45 reactions and 27 species and 5 reactions and 12 species, respectively. In the prediction of pollutants NOx and CO, the full mechanism of 131 reactions is considered to be more reliable. The numerical results indicate that the variation of the maximum flame temperature is within 20 percent as compared with that of the full mechanism of 131 reactions. The chemical compositions of major components such as C3H8, H2O, O2, CO2, and N2 are of the same order of magnitude. However, the concentrations of pollutants are quite different.

  3. Nocturnal Low-Level Jet in a Mountain Basin Complex. Part II: Transport and Diffusion of Tracer under Stable Conditions

    SciTech Connect

    Darby, Lisa S.; Allwine, K Jerry; Banta, Robert M.

    2006-05-01

    Differences in nighttime transport and diffusion of sulfur hexafluoride (SF6) tracer in an urban complex-terrain setting (Salt Lake City, Utah) are investigated using surface and Dopplerlidar wind data, and large-scale surface pressure differences. Interacting scales of motion, as studied through the URBAN 2000 field program combined with the Vertical Transport and Mixing Experiment (VTMX), explained the differences in the tracer behavior during three separate Intensive Operating Periods (IOPs). With an emphasis on nighttime stable boundary layer conditions, these field programs were designed to study flow features responsible for the nighttime transport of airborne substances. This transport has implications for air quality, homeland security, and emergency response issues if the airborne substances are hazardous. The important flow features investigated included thermally forced canyon and slope flows and a low-level jet (LLJ) that dominated the basin-scale winds when the surface pressure gradient was weak. The presence of thermally forced flows contributed to the complexity and hindered the predictability of the tracer motion within and beyond the city. When organized thermally forced flows were present, the tracer tended to stay closer to the city for longer periods of time, even though a strong basin-scale LLJ did develop. When thermally forced flows were short-lived or absent, the basin-scale low-level jet dominated the wind field and enhanced the transport of tracer material out of the city.

  4. Performance of a high-velocity pulse-jet filter, II. Final report Sep 76-Sep 79

    SciTech Connect

    Leith, D.; Ellenbecker, M.J.; First, M.W.; Price, J.M.; Martin, A.

    1980-03-01

    The report gives results of a study of the performance of a high-velocity pulse-jet filter. Such filtration has distinct advantages over low-velocity filtration in that the equipment required to clean a gas stream is reduced in size and initial cost as velocity increases. Although high filtration velocity causes a number of problems, many of them are dealt with in the report. Location of the gas inlet to the filter was found to affect penetration and pressure drop; both were higher for inlets near the bottom of the filter housing. Fabric type was also found to affect performance by affecting the amount and characteristics of the dust deposit accumulated. Fabric surface properties help explain the nature of this deposit. These ideas and others were used to develop a mathematical model for pressure drop in a pulse-jet cleaned filter. The model can be used to predict pressure drop under stable or variable operating conditions, and to predict operating conditions that cause unstable filter operation. An understanding of particle/fiber interactions is essential to understanding the collection characteristics of a felt fabric. Under certain conditions, particles bounce on impact with fibers. An adhesion probability was determined and found to depend on incident particle kinetic energy.

  5. Clinical physiology and mechanism of dizocilpine (MK-801)

    PubMed Central

    Somanathan, Ratnasamy

    2010-01-01

    Dizocilpine (MK-801), an extensively investigated drug possessing secondary amine and benzenoid functions, displays a wide array of biological properties, including anticonvulsant and anesthetic. There is scant discussion of biomechanism. A relevant, important finding is formation of oxidative metabolites in the hydroxylamine and phenolic categories. Analogy to cocaine metabolites suggests participation of redox entities, such as, hydroxylamine, nitroxide and nitrosonium, which can lead to electron transfer and radical formation. There is also similarity to metabolism by 3,3′-iminodipropionitrile and phencyclidine. Alternatively, the phenolic metabolites are well-known precursors of ET quinones. The review documents various physiological effects, mainly involving the central nervous system. Also of interest are the pro- and anti-oxidant properties. Considerable attention has been paid to MK-801 as an antagonist of the N-methyl-D-aspartate receptor in the glutamate category. This aspect is often associated with effects on the central nervous system. The review also provides recent literature dealing with MK-801/NMDA receptor in various areas of bioactivity. Studies were made of MK-801 involvement in working memory processing. Deficits in behavior were noted after administration of the drug. Treatment of mice with dizocilpine induced learning impairment. The influence of MK-801 on fear has been investigated. The substance is known to exert an analgesic effect in pain control. A number of reports deal with anesthetic properties. PMID:20716924

  6. Measurement of the WW+WZ Production Cross Section Using the Lepton+Jets Final State at CDF II

    SciTech Connect

    Aaltonen, T.; Adelman, J.; Alvarez Gonzalez, B.; Amerio, S.; Amidei, D.; Anastassov, A.; Annovi, A.; Antos, J.; Apollinari, G.; Apresyan, A.; Arisawa, T.; /Waseda U. /Dubna, JINR

    2009-11-01

    We report two complementary measurements of the diboson (WW + WZ) cross section in the final state consisting of an electron or muon, missing transverse energy, and jets, performed using p{bar p} collision data at {radical}s = 1.96 TeV collected by the Collider Detector at Fermilab. The first method uses the dijet invariant mass distribution while the second method uses more of the kinematic information in the event through matrix-element calculations of the signal and background processes and has a higher sensitivity. The result from the second method has a signal significance of 5.4{sigma} and is the first observation of WW + WZ production using this signature. Combining the results from both methods gives {sigma}{sub WW+WZ} = 16.0 {+-} 3.3 pb, in agreement with the standard model prediction.

  7. Analysis of Performance of Jet Engine from Characteristics of Components II : Interaction of Components as Determined from Engine Operation

    NASA Technical Reports Server (NTRS)

    Goldstein, Arthur W; Alpert, Sumner; Beede, William; Kovach, Karl

    1949-01-01

    In order to understand the operation and the interaction of jet-engine components during engine operation and to determine how component characteristics may be used to compute engine performance, a method to analyze and to estimate performance of such engines was devised and applied to the study of the characteristics of a research turbojet engine built for this investigation. An attempt was made to correlate turbine performance obtained from engine experiments with that obtained by the simpler procedure of separately calibrating the turbine with cold air as a driving fluid in order to investigate the applicability of component calibration. The system of analysis was also applied to prediction of the engine and component performance with assumed modifications of the burner and bearing characteristics, to prediction of component and engine operation during engine acceleration, and to estimates of the performance of the engine and the components when the exhaust gas was used to drive a power turbine.

  8. Sound generated by instability waves of supersonic flows. I Two-dimensional mixing layers. II - Axisymmetric jets

    NASA Technical Reports Server (NTRS)

    Tam, C. K. W.; Burton, D. E.

    1984-01-01

    An investigation is conducted of the phenomenon of sound generation by spatially growing instability waves in high-speed flows. It is pointed out that this process of noise generation is most effective when the flow is supersonic relative to the ambient speed of sound. The inner and outer asymptotic expansions corresponding to an excited instability wave in a two-dimensional mixing layer and its associated acoustic fields are constructed in terms of the inner and outer spatial variables. In matching the solutions, the intermediate matching principle of Van Dyke and Cole is followed. The validity of the theory is tested by applying it to an axisymmetric supersonic jet and comparing the calculated results with experimental measurements. Very favorable agreements are found both in the calculated instability-wave amplitude distribution (the inner solution) and the near pressure field level contours (the outer solution) in each case.

  9. SAFARI engineering model 50 mK cooler

    NASA Astrophysics Data System (ADS)

    Duband, L.; Duval, J. M.; Luchier, N.

    2014-11-01

    SAFARI is an infrared instrument developed by a European based consortium to be flown in SPICA, a Japanese led mission. The SAFARI detectors are transition edge sensors (TES) and require temperatures down to 50 mK for their operation. For that purpose we have developed a hybrid architecture based on the combination of a 300 mK sorption stage and a small adiabatic demagnetization stage. An engineering model has been designed to provide net heat lifts of 0.4 and 14 μW respectively at 50 and 300 mK, with an overall cycle duration of 48 h and a duty cycle objective of over 75%. The cooler is self-contained, fits in a volume of 156 × 312 × 182 mm and is expected to weigh 5.1 kg. It has been designed to withstand static loads of 120 g and a random vibration level of 21 g RMS.

  10. mkESA: enhanced suffix array construction tool

    PubMed Central

    Homann, Robert; Fleer, David; Giegerich, Robert; Rehmsmeier, Marc

    2009-01-01

    Summary: We introduce the tool mkESA, an open source program for constructing enhanced suffix arrays (ESAs), striving for low memory consumption, yet high practical speed. mkESA is a user-friendly program written in portable C99, based on a parallelized version of the Deep-Shallow suffix array construction algorithm, which is known for its high speed and small memory usage. The tool handles large FASTA files with multiple sequences, and computes suffix arrays and various additional tables, such as the LCP table (longest common prefix) or the inverse suffix array, from given sequence data. Availability: The source code of mkESA is freely available under the terms of the GNU General Public License (GPL) version 2 at http://bibiserv.techfak.uni-bielefeld.de/mkesa/. Contact: rhomann@techfak.uni-bielefeld.de PMID:19246510

  11. Testing of 100 mK bolometers for space applications

    NASA Technical Reports Server (NTRS)

    Murray, A. G.; Ade, P. A. R.; Bhatia, R. S.; Griffin, M. J.; Maffei, B.; Nartallo, R.; Beeman, J. W.; Bock, J.; Lange, A.; DelCastillo, H.

    1996-01-01

    Electrical and optical performance data are presented for a prototype 100 mK spider-web bolometer operating under very low photon backgrounds. These data are compared with the bolometer theory and are used to estimate the expected sensitivity of such a detector used for low background space astronomy. The results demonstrate that the sensitivity and speed of response requirements of the bolometer instruments proposed for these missions can be met by 100 mK spider-web bolometers using neutron transmutation doped germanium as the temperature sensitive element.

  12. mkESA: enhanced suffix array construction tool.

    PubMed

    Homann, Robert; Fleer, David; Giegerich, Robert; Rehmsmeier, Marc

    2009-04-15

    We introduce the tool mkESA, an open source program for constructing enhanced suffix arrays (ESAs), striving for low memory consumption, yet high practical speed. mkESA is a user-friendly program written in portable C99, based on a parallelized version of the Deep-Shallow suffix array construction algorithm, which is known for its high speed and small memory usage. The tool handles large FASTA files with multiple sequences, and computes suffix arrays and various additional tables, such as the LCP table (longest common prefix) or the inverse suffix array, from given sequence data. PMID:19246510

  13. [Air decontamination and the fine filtration system "Potok 150MK"].

    PubMed

    Kapustina, E A; Volodina, E V

    2004-01-01

    Longevity of space stations is dependent on efficiency and robustness of the life support systems. The article describes the principle of operation of air decontamination system Potok 150MK intended for providing microbial safety of the orbital station environment. High quality of air disinfection and decontamination has been demonstrated aboard SS Mir and the International space station. PMID:15233040

  14. Fuzzy jets

    DOE PAGESBeta

    Mackey, Lester; Nachman, Benjamin; Schwartzman, Ariel; Stansbury, Conrad

    2016-06-01

    Here, collimated streams of particles produced in high energy physics experiments are organized using clustering algorithms to form jets . To construct jets, the experimental collaborations based at the Large Hadron Collider (LHC) primarily use agglomerative hierarchical clustering schemes known as sequential recombination. We propose a new class of algorithms for clustering jets that use infrared and collinear safe mixture models. These new algorithms, known as fuzzy jets , are clustered using maximum likelihood techniques and can dynamically determine various properties of jets like their size. We show that the fuzzy jet size adds additional information to conventional jet taggingmore » variables in boosted topologies. Furthermore, we study the impact of pileup and show that with some slight modifications to the algorithm, fuzzy jets can be stable up to high pileup interaction multiplicities.« less

  15. Fuzzy jets

    NASA Astrophysics Data System (ADS)

    Mackey, Lester; Nachman, Benjamin; Schwartzman, Ariel; Stansbury, Conrad

    2016-06-01

    Collimated streams of particles produced in high energy physics experiments are organized using clustering algorithms to form jets. To construct jets, the experimental collaborations based at the Large Hadron Collider (LHC) primarily use agglomerative hierarchical clustering schemes known as sequential recombination. We propose a new class of algorithms for clustering jets that use infrared and collinear safe mixture models. These new algorithms, known as fuzzy jets, are clustered using maximum likelihood techniques and can dynamically determine various properties of jets like their size. We show that the fuzzy jet size adds additional information to conventional jet tagging variables in boosted topologies. Furthermore, we study the impact of pileup and show that with some slight modifications to the algorithm, fuzzy jets can be stable up to high pileup interaction multiplicities.

  16. Photon + jets at D0

    SciTech Connect

    Sonnenschein, Lars; /RWTH Aachen U.

    2009-06-01

    Photon plus jet production has been studied by the D0 experiment in Run II of the Fermilab Tevatron Collider at a centre of mass energy of {radical}s = 1.96 TeV. Measurements of the inclusive photon, inclusive photon plus jet, photon plus heavy flavour jet cross sections and double parton interactions in photon plus three jet events are presented. They are based on integrated luminosities between 0.4 fb{sup -1} and 1.0 fb{sup -1}. The results are compared to perturbative QCD calculations in various approximations.

  17. Search for vector-like quark production in the lepton+jets and dilepton+jets final states using 5.4 fb-1 of Run II data

    SciTech Connect

    Caughron, Seth

    2011-01-01

    The Standard Model of particle physics provides an excellent description of particle interactions at energies up to ~1 TeV, but it is expected to fail above that scale. Multiple models developed to describe phenomena above the TeV scale predict the existence of very massive, vector-like quarks. A search for single electroweak production of such particles in p$\\bar{p}$ collisions at a center-of-mass energy of 1.96 TeV is performed in the W+jets and Z+jets channels. The data were collected by the DØ detector at the Fermilab Tevatron Collider and correspond to an integrated luminosity of 5.4 fb-1. Events consistent with a heavy object decaying to a vector boson and a jet are selected. We observe no significant excess in comparison to the background prediction and set 95% confidence level upper limits on production cross sections for vector-like quarks decaying to W+jet and Z+jet. Assuming a vector-like quark -- standard model quark coupling parameter $\\tilde{κ}$qQ of unity, we exclude vector-like quarks with mass below 693 GeV for decays to W+jet and mass below 449 GeV for decays to Z+jet. These represent the most sensitive limits to date.

  18. Explosive Nucleosynthesis in Magnetohydrodynamical Jets from Collapsars. II --- Heavy-Element Nucleosynthesis of s, p, r-Processes

    NASA Astrophysics Data System (ADS)

    Ono, M.; Hashimoto, M.; Fujimoto, S.; Kotake, K.; Yamada, S.

    2012-10-01

    We investigate the nucleosynthesis in a massive star of 70 M_{⊙} with solar metallicity in the main sequence stage. The helium core mass after hydrogen burning corresponds to 32 M_{⊙}. Nucleosynthesis calculations have been performed during the stellar evolution and the jetlike supernova explosion of a collapsar model. We focus on the production of elements heavier than iron group nuclei. Nucleosynthesis calculations have been accomplished consistently from hydrostatic to dynamic stages by using large nuclear reaction networks, where the weak s-, p-, and r-processes are taken into account. We confirm that s-elements of 60 < A < 90 are highly overproduced relative to the solar abundances in the hydrostatic nucleosynthesis. During oxygen burning, p-elements of A > 90 are produced via photodisintegrations of seed s-elements. However, the produced p-elements are disintegrated in later stages except for ^{180}Ta. In the explosive nucleosynthesis, elements of 90 < A < 160 are significantly overproduced relative to the solar values owing to the r-process, which is very different from the results of spherical explosion models. Only heavy p-elements (N > 50) are overproduced via the p-process because of the low peak temperatures in the oxygen- and neon-rich layers. Compared with the previous study of r-process nucleosynthesis calculations in the collapsar model of 40 M_{⊙} by Fujimoto et al. [S. Fujimoto, M. Hashimoto, K. Kotake and S. Yamada, Astrophys. J. 656 (2007), 382; S. Fujimoto, N. Nishimura and M. Hashimoto, Astrophys. J. 680 (2008), 1350], our jet model cannot contribute to the third peak of the solar r-elements and intermediate p-elements, which have been much produced because of the distribution of the lowest part of electron fraction in the ejecta. Averaging the overproduction factors over the progenitor masses with the use of Salpeter's IMF, we suggest that the 70 M_{⊙} star could contribute to the solar weak s}-elements of 60 < A < 90 and neutron

  19. Falciparum malaria parasites invade erythrocytes that lack glycophorin A and B (MkMk). Strain differences indicate receptor heterogeneity and two pathways for invasion.

    PubMed Central

    Hadley, T J; Klotz, F W; Pasvol, G; Haynes, J D; McGinniss, M H; Okubo, Y; Miller, L H

    1987-01-01

    To determine the ligands on erythrocytes for invasion by Plasmodium falciparum, we tested invasion into MkMk erythrocytes that lack glycophorins A and B and enzyme-treated erythrocytes by parasites that differ in their requirement for erythrocyte sialic acid. The 7G8 strain invaded MkMk erythrocytes and neuraminidase-treated normal erythrocytes with greater than 50% the efficiency of normal erythrocytes. In contrast, the Camp strain invaded MkMk erythrocytes at 20% of control and neuraminidase-treated normal erythrocytes at only 1.8% of control. Invasion of MkMk erythrocytes by 7G8 parasites was unaffected by treatment with neuraminidase but was markedly reduced by treatment with trypsin. In contrast, invasion of MkMk cells by Camp parasites was markedly reduced by neuraminidase but was unaffected by trypsin. We conclude that the 7G8 and Camp strains differ in ligand requirements for invasion and that 7G8 requires a trypsin sensitive ligand distinct from glycophorins A and B. Images PMID:3308959

  20. Inclusive jet production at the Tevatron

    SciTech Connect

    Norniella, Olga; /Barcelona, IFAE

    2006-08-01

    Preliminary results on inclusive jet production in proton-antiproton collisions at {radical}s = 1.96 TeV based on 1 fb{sup -1} of CDF Run II data are presented. Measurements are preformed using different jet algorithms in a wide range of jet transverse momentum and jet rapidity. The measured cross sections are compared to next-to-leading order perturbative QCD calculations

  1. Triggering on electrons, jets and tau leptons with the CMS upgraded calorimeter trigger for the LHC RUN II

    NASA Astrophysics Data System (ADS)

    Zabi, A.; Beaudette, F.; Cadamuro, L.; Mastrolorenzo, L.; Romanteau, T.; Sauvan, J. B.; Strebler, T.; Marrouche, J.; Wardle, N.; Aggleton, R.; Ball, F.; Brooke, J.; Newbold, D.; Paramesvaran, S.; Smith, D.; Baber, M.; Bundock, A.; Citron, M.; Elwood, A.; Hall, G.; Iles, G.; Laner, C.; Penning, B.; Rose, A.; Tapper, A.; Durkin, T.; Harder, K.; Harper, S.; Shepherd-Themistocleous, C.; Thea, A.; Williams, T.

    2016-02-01

    The Compact Muon Solenoid (CMS) experiment has implemented a sophisticated two-level online selection system that achieves a rejection factor of nearly 105. During Run II, the LHC will increase its centre-of-mass energy up to 13 TeV and progressively reach an instantaneous luminosity of 2 × 1034 cm-2 s-1. In order to guarantee a successful and ambitious physics programme under this intense environment, the CMS Trigger and Data acquisition (DAQ) system has been upgraded. A novel concept for the L1 calorimeter trigger is introduced: the Time Multiplexed Trigger (TMT) . In this design, nine main processors receive each all of the calorimeter data from an entire event provided by 18 preprocessors. This design is not different from that of the CMS DAQ and HLT systems. The advantage of the TMT architecture is that a global view and full granularity of the calorimeters can be exploited by sophisticated algorithms. The goal is to maintain the current thresholds for calorimeter objects and improve the performance for their selection. The performance of these algorithms will be demonstrated, both in terms of efficiency and rate reduction. The callenging aspects of the pile-up mitigation and firmware design will be presented.

  2. Search for Third Generation Squarks in the Missing Transverse Energy plus Jet Sample at CDF Run II

    SciTech Connect

    Marono, Miguel Vidal

    2010-03-01

    lightest SUSY particle (LSP) which would provide a candidate for cold dark matter, that account for 23% of the universe content, as strongly suggested by recent astrophysical data [1]. The Tevatron is a hadron collider operating at Fermilab, USA. This accelerator provides proton-antiproton (p$\\bar{p}$) collisions with a center of mass energy of √s = 1.96 TeV. CDF and D0 are the detectors built to analyse the products of the collisions provided by the Tevatron. Both experiments have produced a very significant scientific output in the last few years, like the discovery of the top quark or the measurement of the Bs mixing. The Tevatron experiments are also reaching sensitivity to the SM Higgs boson. The scientific program of CDF includes a broad spectrum on searches for physics signatures beyond the Standard Model. Tevatron is still the energy frontier, what means an unique opportunity to produce a discovery in physic beyond the Standard Model. The analyses presented in this thesis focus on the search for third generation squarks in the missing transverse energy plus jets final state. The production of sbottom ($\\tilde{b}$) and stop ($\\tilde{t}$) quarks could be highly enhanced at the Tevatron, giving the possibility of discovering new physics or limiting the parameter space available in the theory. No signal is found over the predicted Standard Model background in both searches. Instead, 95% confidence level limits are set on the production cross section, and then translated into the mass plane of the hypothetical particles. This thesis sketches the basic theory concepts of the Standard Model and the Minimal Supersymmetric Extension in Chapter 2. Chapter 3, describes the Tevatron and CDF. Based on the CDF subsystems information, Chapter 4 and 5 describe the analysis objet reconstruction and the heavy flavor tagging tools. The development of the analyses is shown in Chapter 6 and Chapter 7. Finally, Chapter 8 is devoted to discuss the results and conclusions

  3. Discovery of MK-7246, a selective CRTH2 antagonist for the treatment of respiratory diseases.

    PubMed

    Gallant, Michel; Beaulieu, Christian; Berthelette, Carl; Colucci, John; Crackower, Michael A; Dalton, Chad; Denis, Danielle; Ducharme, Yves; Friesen, Richard W; Guay, Daniel; Gervais, François G; Hamel, Martine; Houle, Robert; Krawczyk, Connie M; Kosjek, Birgit; Lau, Stephen; Leblanc, Yves; Lee, Ernest E; Levesque, Jean-François; Mellon, Christophe; Molinaro, Carmela; Mullet, Wayne; O'Neill, Gary P; O'Shea, Paul; Sawyer, Nicole; Sillaots, Susan; Simard, Daniel; Slipetz, Deborah; Stocco, Rino; Sørensen, Dan; Truong, Vouy Linh; Wong, Elizabeth; Wu, Jin; Zaghdane, Helmi; Wang, Zhaoyin

    2011-01-01

    In this manuscript we wish to report the discovery of MK-7246 (4), a potent and selective CRTH2 (DP2) antagonist. SAR studies leading to MK-7246 along with two synthetic sequences enabling the preparation of this novel class of CRTH2 antagonist are reported. Finally, the pharmacokinetic and metabolic profile of MK-7246 is disclosed. PMID:21106375

  4. Cosmic jets

    SciTech Connect

    Blandford, R.D.; Begelman, M.C.; Rees, M.J.

    1982-05-01

    Observations with radio telescopes have revealed that the center of many galaxies is a place of violent activity. This activity is often manifested in the production of cosmic jets. Each jet is a narrow stream of plasma that appears to squirt out of the center of a galaxy emitting radiowaves as it does so. New techniques in radio astronomy have shown how common jets are in the universe. These jets take on many different forms. The discovery of radio jets has helped in the understanding of the double structure of the majority of extragalactic radio sources. The morphology of some jets and explanations of how jets are fueled are discussed. There are many difficulties plaguing the investigation of jets. Some of these difficulties are (1) it is not known how much power the jets are radiating, (2) it is hard to tell whether a jet delieated by radio emission is identical to the region where ionized gas is flowing, and (3) what makes them. (SC)

  5. MK3 Modulation Affects BMI1-Dependent and Independent Cell Cycle Check-Points

    PubMed Central

    Dahlmans, Vivian E. H.; Spaapen, Frank; Salvaing, Juliette; Vanhove, Jolien; Geijselaers, Claudia; Bartels, Stefanie J. J.; Partouns, Iris; Neumann, Dietbert; Speel, Ernst-Jan; Takihara, Yoshihiro; Wouters, Bradly G.; Voncken, Jan Willem

    2015-01-01

    Although the MK3 gene was originally found deleted in some cancers, it is highly expressed in others. The relevance of MK3 for oncogenesis is currently not clear. We recently reported that MK3 controls ERK activity via a negative feedback mechanism. This prompted us to investigate a potential role for MK3 in cell proliferation. We here show that overexpression of MK3 induces a proliferative arrest in normal diploid human fibroblasts, characterized by enhanced expression of replication stress- and senescence-associated markers. Surprisingly, MK3 depletion evokes similar senescence characteristics in the fibroblast model. We previously identified MK3 as a binding partner of Polycomb Repressive Complex 1 (PRC1) proteins. In the current study we show that MK3 overexpression results in reduced cellular EZH2 levels and concomitant loss of epigenetic H3K27me3-marking and PRC1/chromatin-occupation at the CDKN2A/INK4A locus. In agreement with this, the PRC1 oncoprotein BMI1, but not the PCR2 protein EZH2, bypasses MK3-induced senescence in fibroblasts and suppresses P16INK4A expression. In contrast, BMI1 does not rescue the MK3 loss-of-function phenotype, suggesting the involvement of multiple different checkpoints in gain and loss of MK3 function. Notably, MK3 ablation enhances proliferation in two different cancer cells. Finally, the fibroblast model was used to evaluate the effect of potential tumorigenic MK3 driver-mutations on cell proliferation and M/SAPK signaling imbalance. Taken together, our findings support a role for MK3 in control of proliferation and replicative life-span, in part through concerted action with BMI1, and suggest that the effect of MK3 modulation or mutation on M/SAPK signaling and, ultimately, proliferation, is cell context-dependent. PMID:25853770

  6. Jet maximization, axis minimization, and stable cone finding

    NASA Astrophysics Data System (ADS)

    Thaler, Jesse

    2015-10-01

    Jet finding is a type of optimization problem, where hadrons from a high-energy collision event are grouped into jets based on a clustering criterion. As three interesting examples, one can form a jet cluster that (i) optimizes the overall jet four-vector, (ii) optimizes the jet axis, or (iii) aligns the jet axis with the jet four-vector. In this paper, we show that these three approaches to jet finding, despite being philosophically quite different, can be regarded as descendants of a mother optimization problem. For the special case of finding a single cone jet of fixed opening angle, the three approaches are genuinely identical when defined appropriately, and the result is a stable cone jet with the largest value of a quantity J . This relationship is only approximate for cone jets in the rapidity-azimuth plane, as used at the Large Hadron Collider, though the differences are mild for small radius jets.

  7. Water Jetting

    NASA Technical Reports Server (NTRS)

    1985-01-01

    Hi-Tech Inc., a company which manufactures water jetting equipment, needed a high pressure rotating swivel, but found that available hardware for the system was unsatisfactory. They were assisted by Marshall, which had developed water jetting technology to clean the Space Shuttles. The result was a completely automatic water jetting system which cuts rock and granite and removes concrete. Labor costs have been reduced; dust is suppressed and production has been increased.

  8. Cosmic jets

    NASA Technical Reports Server (NTRS)

    Rees, M. J.

    1986-01-01

    The evidence that active galactic nuclei produce collimated plasma jets is summarised. The strongest radio galaxies are probably energised by relativistic plasma jets generated by spinning black holes interacting with magnetic fields attached to infalling matter. Such objects can produce e(+)-e(-) plasma, and may be relevant to the acceleration of the highest-energy cosmic ray primaries. Small-scale counterparts of the jet phenomenon within our own galaxy are briefly reviewed.

  9. Osmotic stress induces the phosphorylation of WNK4 Ser575 via the p38MAPK-MK pathway

    PubMed Central

    Maruyama, Junichi; Kobayashi, Yumie; Umeda, Tsuyoshi; Vandewalle, Alain; Takeda, Kohsuke; Ichijo, Hidenori; Naguro, Isao

    2016-01-01

    The With No lysine [K] (WNK)-Ste20-related proline/alanine-rich kinase (SPAK)/oxidative stress-responsive kinase 1 (OSR1) pathway has been reported to be a crucial signaling pathway for triggering pseudohypoaldosteronism type II (PHAII), an autosomal dominant hereditary disease that is characterized by hypertension. However, the molecular mechanism(s) by which the WNK-SPAK/OSR1 pathway is regulated remain unclear. In this report, we identified WNK4 as an interacting partner of a recently identified MAP3K, apoptosis signal-regulating kinase 3 (ASK3). We found that WNK4 is phosphorylated in an ASK3 kinase activity-dependent manner. By exploring the ASK3-dependent phosphorylation sites, we identified Ser575 as a novel phosphorylation site in WNK4 by LC-MS/MS analysis. ASK3-dependent WNK4 Ser575 phosphorylation was mediated by the p38MAPK-MAPK-activated protein kinase (MK) pathway. Osmotic stress, as well as hypotonic low-chloride stimulation, increased WNK4 Ser575 phosphorylation via the p38MAPK-MK pathway. ASK3 was required for the p38MAPK activation induced by hypotonic stimulation but was not required for that induced by hypertonic stimulation or hypotonic low-chloride stimulation. Our results suggest that the p38MAPK-MK pathway might regulate WNK4 in an osmotic stress-dependent manner but its upstream regulators might be divergent depending on the types of osmotic stimuli. PMID:26732173

  10. p38MAPK/MK2-mediated phosphorylation of RBM7 regulates the human nuclear exosome targeting complex

    PubMed Central

    Lubas, Michal; Tehrani, Mohammad; Menon, Manoj B.; Ronkina, Natalia; Rousseau, Simon; Cohen, Philip; Kotlyarov, Alexey

    2015-01-01

    The nuclear exosome targeting complex (NEXT) directs a major 3′–5′ exonuclease, the RNA exosome, for degradation of nuclear noncoding (nc) RNAs. We identified the RNA-binding component of the NEXT complex, RBM7, as a substrate of p38MAPK/MK2-mediated phosphorylation at residue S136. As a result of this phosphorylation, RBM7 displays a strongly decreased RNA-binding capacity, while inhibition of p38MAPK or mutation of S136A in RBM7 increases its RNA association. Interestingly, promoter-upstream transcripts (PROMPTs), such as proRBM39, proEXT1, proDNAJB4, accumulated upon stress stimulation in a p38MAPK/MK2-dependent manner, a process inhibited by overexpression of RBM7S136A. While there are no stress-dependent changes in RNA-polymerase II (RNAPII) occupation of PROMPT regions representing unchanged transcription, stability of PROMPTs is increased. Hence, we propose that phosphorylation of RBM7 by the p38MAPK/MK2 axis increases nuclear ncRNA stability by blocking their RBM7-binding and subsequent RNA exosome targeting to allow stress-dependent modulations of the noncoding transcriptome. PMID:25525152

  11. Unconditional jetting.

    PubMed

    Gañán-Calvo, Alfonso M

    2008-08-01

    Capillary jetting of a fluid dispersed into another immiscible phase is usually limited by a critical capillary number, a function of the Reynolds number and the fluid property ratios. Critical conditions are set when the minimum spreading velocity of small perturbations v_{-};{*} along the jet (marginal stability velocity) is zero. Here we identify and describe parametric regions of high technological relevance, where v_{-};{*}>0 and the jet flow is always supercritical independently of the dispersed liquid flow rate; within these relatively broad regions, the jet does not undergo the usual dripping-jetting transition, so that either the jet can be made arbitrarily thin (yielding droplets of any imaginably small size), or the issuing flow rate can be made arbitrarily small. In this work, we provide illustrative analytical studies of asymptotic cases for both negligible and dominant inertia forces. In this latter case, requiring a nonzero jet surface velocity, axisymmetric perturbation waves "surf" downstream for all given wave numbers, while the liquid bulk can remain static. In the former case (implying small Reynolds flow) we found that the jet profile small slope is limited by a critical value; different published experiments support our predictions. PMID:18850933

  12. Isolation of the Entomopathogenic Fungal Strain Cod-MK1201 from a Cicada Nymph and Assessment of Its Antibacterial Activities.

    PubMed

    Sangdee, Kusavadee; Nakbanpote, Woranan; Sangdee, Aphidech

    2015-01-01

    The entomopathogenic fungus Cod-MK1201 was isolated from a dead cicada nymph. Three regions of ribosomal nuclear DNA, the internal transcribed spacers of nuclear ribosomal DNA repeats (ITS), the partial small subunit of rDNA (nrSSU) , and the partial large subunit of rDNA (nrLSU), and two protein-coding regions, the elongation factor 1α (EF-1α), and the largest subunit of the RNA polymerase II (rpb1) gene, were sequenced and used for fungal identification. The phylogenetic analysis of the ITS and the combined data set of the five genes indicated that the fungal isolate Cod-MK1201 is a new strain of Cordyceps sp. that is closely related to Cordyceps nipponica and C. kanzashiana. Crude extracts of mycelium-cultured Cod-MK1201 were obtained using distilled water and 50% (v/v) ethanol, and the antibacterial activity of each was determined. Both extracts had activity against Gram-positive and Gram-negative bacteria, but the ethanol extract was the more potent of the two. The antibacterial activity of the protein fractions of these extracts was also determined. The protein fraction from the ethanol extract was more antibacterial than the protein fraction from the aqueous extract. Three antibacterial constituents including adenosine, the total phenolic content (TPC), and the total flavonoid content (TFC) was also determined. The results showed that the adenosine content, the TPC, and the TFC of the ethanol extract were more active than those of the aqueous extract. Moreover, synergism was detected between these antibacterial constituents. In conclusion, the entomopathogenic fungal isolate Cod-MK1201 represents a natural source of antibacterial agents. PMID:25746406

  13. Inclusive jet production using the kt algorithm

    SciTech Connect

    Norniella, Olga; /Barcelona, IFAE

    2006-05-01

    Results on inclusive jet production using the k{sub T} algorithm in proton-antiproton collisions at {radical}s = 1.96 TeV are presented, based on 1 fb{sup -1} of CDF Run II data. The measurements are carried out for jets with p{sub T}{sup jet} > 54 GeV/c in five different jet rapidity regions up to |y{sub jet}| = 2.1. The measured cross sections are corrected to the hadron level and compared to next-to-leading order perturbative QCD predictions (NLO pQCD).

  14. Hot Electrons and Energy Transport in Metals at MK Temperatures.

    NASA Astrophysics Data System (ADS)

    Roukes, Michael Lee

    Using a new technique involving the generation of hot carriers, we directly measure energy loss lifetimes for electrons in impure metals at mK temperatures. At these temperatures very weak inelastic scattering processes determine energy transport out of the electron gas. A temperature difference between the electron gas and the lattice can be induced by applying an extremely small electric field (of order 1 (mu)V/cm at 25 mK). This temperature difference reflects the rate at which electrons lose energy to the surroundings. The experiment is carried out using a pair of interdigitated thin film resistors mounted on a millidegree demagnetization cryostat: we obtain electron temperature directly by observing current fluctuations. Noise generated by the resistors is measured using an ultra-sensitive two -channel dc SQUID system, providing femtoamp resolution at KHz frequencies. A dc voltage applied across one resistor imposes the bias field causing electron heating. Phonon temperature in the metal lattice is obtained by measuring noise from a second (unbiased) resistor, which is tightly coupled thermally to the first (biased). Our measurements show that electron heating follows an E('2/5) power law in the regime where electron temperature is largely determined by the electric field, E. This implies a T('-3) law for the energy loss lifetime, suggesting electron -acoustic phonon processes dominate. In the mK temperature regime the conductivity is impurity limited and remains ohmic, even as the electrons heat. Assuming a T('3) dependence and extrapolating our measured rates to higher temperatures, we find agreement with electron-phonon rates measured above 1K in clean bulk metals. This contrasts with results from weak localization experiments showing a power law differing from T('3) and much faster rates. This difference arises because weak localization experiments measure the electron phase coherence lifetime; our electron heating experiments, however, measure an energy

  15. Investigation of rocket motors 3 inch number 1 Mk 4

    NASA Astrophysics Data System (ADS)

    Barrington, L. M.

    1994-05-01

    In 1992, Aircraft Research and Development Unit (ARDU), RAAF, experienced two misfires with Rocket Motors, 3 in., No. 1, Mk 4, during a series of firings at Woomera. These motors were sampled from a batch manufactured in 1957, and subsequent to the misfires this batch was withdrawn from use. An alternate batch of motors manufactured in 1966 was available to ARDU. Tests were conducted on a number of these motors to advise on their suitability for use, and as a result, a further five years life was assigned with a recommendation to retest after that period.

  16. Equilibrium shape of 4He crystal under zero gravity below 200 mK

    PubMed Central

    Takahashi, Takuya; Ohuchi, Haruka; Nomura, Ryuji; Okuda, Yuichi

    2015-01-01

    Equilibrium crystal shape is the lowest energy crystal shape that is hardly realized in ordinary crystals because of their slow relaxation. 4He quantum crystals in a superfluid have been expected as unique exceptions that grow extremely fast at very low temperatures. However, on the ground, gravity considerably deforms the crystals and conceals the equilibrium crystal shape, and thus, gravity-free environment is needed to observe the equilibrium shape of 4He. We report the relaxation processes of macroscopic 4He crystals in a superfluid below 200 mK under zero gravity using a parabolic flight of a jet plane. When gravity was removed from a gravity-flattened 4He crystal, the crystal rapidly transformed into a shape with flat surfaces. Although the relaxation processes were highly dependent on the initial condition, the crystals relaxed to a nearly homothetic shape in the end, indicating that they were truly in an equilibrium shape minimizing the interfacial free energy. Thanks to the equilibrium shape, we were able to determine the Wulff’s origin and the size of the c-facet together with the vicinal surface profile next to the c-facet. The c-facet size was extremely small in the quantum crystals, and the facet-like flat surfaces were found to be the vicinal surfaces. At the same time, the interfacial free energy of the a-facet and s-facet was also obtained. PMID:26601315

  17. Equilibrium shape of (4)He crystal under zero gravity below 200 mK.

    PubMed

    Takahashi, Takuya; Ohuchi, Haruka; Nomura, Ryuji; Okuda, Yuichi

    2015-10-01

    Equilibrium crystal shape is the lowest energy crystal shape that is hardly realized in ordinary crystals because of their slow relaxation. (4)He quantum crystals in a superfluid have been expected as unique exceptions that grow extremely fast at very low temperatures. However, on the ground, gravity considerably deforms the crystals and conceals the equilibrium crystal shape, and thus, gravity-free environment is needed to observe the equilibrium shape of (4)He. We report the relaxation processes of macroscopic (4)He crystals in a superfluid below 200 mK under zero gravity using a parabolic flight of a jet plane. When gravity was removed from a gravity-flattened (4)He crystal, the crystal rapidly transformed into a shape with flat surfaces. Although the relaxation processes were highly dependent on the initial condition, the crystals relaxed to a nearly homothetic shape in the end, indicating that they were truly in an equilibrium shape minimizing the interfacial free energy. Thanks to the equilibrium shape, we were able to determine the Wulff's origin and the size of the c-facet together with the vicinal surface profile next to the c-facet. The c-facet size was extremely small in the quantum crystals, and the facet-like flat surfaces were found to be the vicinal surfaces. At the same time, the interfacial free energy of the a-facet and s-facet was also obtained. PMID:26601315

  18. MK2 Deletion in Mice Prevents Diabetes-Induced Perturbations in Lipid Metabolism and Cardiac Dysfunction.

    PubMed

    Ruiz, Matthieu; Coderre, Lise; Lachance, Dominic; Houde, Valérie; Martel, Cécile; Thompson Legault, Julie; Gillis, Marc-Antoine; Bouchard, Bertrand; Daneault, Caroline; Carpentier, André C; Gaestel, Matthias; Allen, Bruce G; Des Rosiers, Christine

    2016-02-01

    Heart disease remains a major complication of diabetes, and the identification of new therapeutic targets is essential. This study investigates the role of the protein kinase MK2, a p38 mitogen-activated protein kinase downstream target, in the development of diabetes-induced cardiomyopathy. Diabetes was induced in control (MK2(+/+)) and MK2-null (MK2(-/-)) mice using repeated injections of a low dose of streptozotocin (STZ). This protocol generated in MK2(+/+) mice a model of diabetes characterized by a 50% decrease in plasma insulin, hyperglycemia, and insulin resistance (IR), as well as major contractile dysfunction, which was associated with alterations in proteins involved in calcium handling. While MK2(-/-)-STZ mice remained hyperglycemic, they showed improved IR and none of the cardiac functional or molecular alterations. Further analyses highlighted marked lipid perturbations in MK2(+/+)-STZ mice, which encompass increased 1) circulating levels of free fatty acid, ketone bodies, and long-chain acylcarnitines and 2) cardiac triglyceride accumulation and ex vivo palmitate β-oxidation. MK2(-/-)-STZ mice were also protected against all these diabetes-induced lipid alterations. Our results demonstrate the benefits of MK2 deletion on diabetes-induced cardiac molecular and lipid metabolic changes, as well as contractile dysfunction. As a result, MK2 represents a new potential therapeutic target to prevent diabetes-induced cardiac dysfunction. PMID:26558681

  19. Experiment on performance of adjustable jet pump

    NASA Astrophysics Data System (ADS)

    Zhu, J. M.; Long, X. P.; Zhang, S. B.; Lu, X.

    2012-11-01

    When the water level of upper or lower reaches of hydraulic power station changes, the adjustable jet pump which is different from traditional fixed jet pump can maintain stable pressure and flow rate for the system of technical water supply of hydraulic power plant. The model test indicates that the efficiency of the adjustable jet pump is slightly lower than fixed jet pump near rating operation point. With the decrease of opening degree, both efficiencies are more and more close to each other. The fundamental performance of I-type adjustable jet pump is better than II-type and the cavitation performance of I-type adjustable jet pump is worse than II-type. Test data also indicate that the performance of adjustable jet pump is very different from fixed jet pump, so the theory of fixed jet pump is not able to be copied to adjustable jet pump. It is necessary to farther study on the performance of the adjustable jet pump. This paper has reference value for analogous design of system of circulation water supply to turbine units in hydraulic power station.

  20. CaMK4 Gene Deletion Induces Hypertension

    PubMed Central

    Santulli, Gaetano; Cipolletta, Ersilia; Sorriento, Daniela; Del Giudice, Carmine; Anastasio, Antonio; Monaco, Sara; Maione, Angela Serena; Condorelli, Gianluigi; Puca, Annibale; Trimarco, Bruno; Illario, Maddalena; Iaccarino, Guido

    2012-01-01

    Background The expression of calcium/calmodulin-dependent kinase IV (CaMKIV) was hitherto thought to be confined to the nervous system. However, a recent genome-wide analysis indicated an association between hypertension and a single-nucleotide polymorphism (rs10491334) of the human CaMKIV gene (CaMK4), which suggests a role for this kinase in the regulation of vascular tone. Methods and Results To directly assess the role of CaMKIV in hypertension, we characterized the cardiovascular phenotype of CaMK4−/− mice. They displayed a typical hypertensive phenotype, including high blood pressure levels, cardiac hypertrophy, vascular and kidney damage, and reduced tolerance to chronic ischemia and myocardial infarction compared with wild-type littermates. Interestingly, in vitro experiments showed the ability of this kinase to activate endothelial nitric oxide synthase. Eventually, in a population study, we found that the rs10491334 variant associates with a reduction in the expression levels of CaMKIV in lymphocytes from hypertensive patients. Conclusions Taken together, our results provide evidence that CaMKIV plays a pivotal role in blood pressure regulation through the control of endothelial nitric oxide synthase activity. (J Am Heart Assoc. 2012;1:e001081 doi: 10.1161/JAHA.112.001081.) PMID:23130158

  1. Giant Radio Sources as a Probe of the Cosmological Evolution of the IGM. II. The Observational Constraint on the Model of Radio-Jets Propagation through the X-ray Halo-IGM Interface

    NASA Astrophysics Data System (ADS)

    Kuligowska, E.; Jamrozy, M.; Koziel-Wierzbowska, D.; Machalski, J.

    2009-12-01

    Three limited samples of high-redshift radio sources of FRII-type are used to constrain the dynamical model for the jets' propagation through the two-media environment: the X-ray emitting halo with the power-law density profile surrounding the parent galaxy and the much hotter intergalactic medium (IGM) of a constant density. The model, originally developed by Gopal-Krishna and Wiita, is modified adopting modern values of its free parameters taken from recent X-ray measurements with the XMM-Newton and Chandra Observatories. We find that (i) giant-sized radio sources (≍1 Mpc) exist at redshifts up to z≍2, (ii) all newly identified the largest radio sources with 1jets than peculiar environmental conditions (e.g., voids) in the IGM. The extreme powerful jets may testify to a dominant role of the accretion processes onto black holes in earlier cosmological epochs.

  2. MHD simulations of NSNS mergers in full GR: the role of the initial B field on the emergence of sGRB jets II

    NASA Astrophysics Data System (ADS)

    Lang, Ryan; Paschalidis, Vasileios; Ruiz, Milton; Shapiro, Stuart

    2016-03-01

    Mergers of neutron star-neutron star (NSNS) binaries are among the most promising candidates for the engines that power short gamma-ray bursts (sGRBs). The most likely sGRB model requires the presence of a highly relativistic jet. However, recent relativistic simulations of NSNS mergers have shown that it is difficult for such jets to emerge following the delayed collapse of the hypermassive neutron star (HMNS) remnant. Varying the initial NS magnetic field in an NSNS binary, we have performed magnetohydrodynamic simulations in full general relativity that explore the formation of jets from the black hole-disk system following the HMNS collapse. In this talk we focus on initial dipole magnetic fields which extend from the neutron star interior into the exterior.

  3. 2,4-Diaminopyrimidine MK2 inhibitors. Part I: Observation of an unexpected inhibitor binding mode

    SciTech Connect

    Argiriadi, Maria A.; Ericsson, Anna M.; Harris, Christopher M.; Banach, David L.; Borhani, David W.; Calderwood, David J.; Demers, Megan D.; DiMauro, Jennifer; Dixon, Richard W.; Hardman, Jennifer; Kwak, Silvia; Li, Biqin; Mankovich, John A.; Marcotte, Douglas; Mullen, Kelly D.; Ni, Baofu; Pietras, M.; Sadhukhan, Ramkrishna; Sousa, Silvino; Tomlinson, Medha J.; Wang, L.; Xiang, T.; Talanian, R.V.

    2010-09-17

    MK2 is a Ser/Thr kinase of significant interest as an anti-inflammatory drug discovery target. Here we describe the development of in vitro tools for the identification and characterization of MK2 inhibitors, including validation of inhibitor interactions with the crystallography construct and determination of the unique binding mode of 2,4-diaminopyrimidine inhibitors in the MK2 active site.

  4. Jet-Induced Star Formation

    SciTech Connect

    van Breugel, W; Fragile, C; Anninos, P; Murray, S

    2003-12-16

    Jets from radio galaxies can have dramatic effects on the medium through which they propagate. We review observational evidence for jet-induced star formation in low ('FR-I') and high ('FR-II') luminosity radio galaxies, at low and high redshifts respectively. We then discuss numerical simulations which are aimed to explain a jet-induced starburst ('Minkowski's Object') in the nearby FR-I type radio galaxy NGC 541. We conclude that jets can induce star formation in moderately dense (10 cm{sup -3}), warm (10{sup 4} K) gas; that this may be more common in the dense environments of forming, active galaxies; and that this may provide a mechanism for 'positive' feedback from AGN in the galaxy formation process.

  5. Synthetic Jets

    NASA Technical Reports Server (NTRS)

    Milanovic, Ivana M.

    2003-01-01

    Current investigation of synthetic jets and synthetic jets in cross-flow examined the effects of orifice geometry and dimensions, momentum-flux ratio, cluster of orifices, pitch and yaw angles as well as streamwise development of the flow field. This comprehensive study provided much needed experimental information related to the various control strategies. The results of the current investigation on isolated and clustered synthetic jets with and without cross-flow will be further analyzed and documented in detail. Presentations at national conferences and publication of peer- reviewed journal articles are also expected. Projected publications will present both the mean and turbulent properties of the flow field, comparisons made with the data available in an open literature, as well as recommendations for the future work.

  6. MK2 inhibitor reduces alkali burn-induced inflammation in rat cornea

    PubMed Central

    Chen, Yanfeng; Yang, Wenzhao; Zhang, Xiaobo; Yang, Shu; Peng, Gao; Wu, Ting; Zhou, Yueping; Huang, Caihong; Reinach, Peter S.; Li, Wei; Liu, Zuguo

    2016-01-01

    MK2 activation by p38 MAPK selectively induces inflammation in various diseases. We determined if a MK2 inhibitor (MK2i), improves cornea wound healing by inhibiting inflammation caused by burning rat corneas with alkali. Our study, for the first time, demonstrated that MK2i inhibited alkali burn-induced MK2 activation as well as rises in inflammation based on: a) blunting rises in inflammatory index, inflammatory cell infiltration, ED1+ macrophage and PMN+ neutrophil infiltration; b) suppressing IL-6 and IL-1β gene expression along with those of macrophage inflammatory protein-1α (MIP-1α), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1); c) reducing angiogenic gene expression levels and neovascularization (NV) whereas anti-angiogenic PEDF levels increased. In addition, this study found that MK2i did not affect human corneal epithelial cell (HCEC) proliferation and migration and had no detectable side effects on ocular surface integrity. Taken together, MK2i selectively inhibited alkali burn-induced corneal inflammation by blocking MK2 activation, these effects have clinical relevance in the treatment of inflammation related ocular surface diseases. PMID:27329698

  7. Structure-based lead identification of ATP-competitive MK2 inhibitors.

    PubMed

    Barf, Tjeerd; Kaptein, Allard; de Wilde, Sander; van der Heijden, Ruud; van Someren, Richard; Demont, Dennis; Schultz-Fademrecht, Carsten; Versteegh, Judith; van Zeeland, Mario; Seegers, Nicole; Kazemier, Bert; van de Kar, Bas; van Hoek, Maaike; de Roos, Jeroen; Klop, Henri; Smeets, Ruben; Hofstra, Claudia; Hornberg, Jorrit; Oubrie, Arthur

    2011-06-15

    MK2 kinase is a promising drug discovery target for the treatment of inflammatory diseases. Here, we describe the discovery of novel MK2 inhibitors using X-ray crystallography and structure-based drug design. The lead has in vivo efficacy in a short-term preclinical model. PMID:21565500

  8. STS-42 Commander Grabe uses DTO 653 MK1 Rowing Machine on OV-103's middeck

    NASA Technical Reports Server (NTRS)

    1992-01-01

    STS-42 Commander Ronald J. Grabe exercises using MK1 Rowing Machine on the middeck of Discovery, Orbiter Vehicle (OV) 103. Grabe is using the exercise device as part of Development Test Objective (DTO) 653, Evaluation of MK1 Rowing Machine. The forward lockers appear at Grabe's right and the sleep station behind him.

  9. Benzothiophene inhibitors of MK2. Part 2: Improvements in kinase selectivity and cell potency

    SciTech Connect

    Anderson, David R.; Meyers, Marvin J.; Kurumbail, Ravi G.; Caspers, Nicole; Poda, Gennadiy I.; Long, Scott A.; Pierce, Betsy S.; Mahoney, Matthew W.; Mourey, Robert J.; Parikh, Mihir D.; Pfizer

    2010-10-01

    Optimization of kinase selectivity for a set of benzothiophene MK2 inhibitors provided analogs with potencies of less than 500 nM in a cell based assay. The selectivity of the inhibitors can be rationalized by examination of X-ray crystal structures of inhibitors bound to MK2.

  10. {sup 3}He melting pressure temperature scale below 25 mK

    SciTech Connect

    Adams, E.D.; Ni, W.; Xia, J.S.

    1995-04-01

    Using {sup 60}Co {gamma} ray anisotropy radiation as a primary thermometer, with a Pt NMR susceptibility secondary thermometer, the authors have made high precision measurements of the {sup 3}He melting pressure versus temperature from 500 {mu}K to 25 mK. Temperatures obtained for the fixed points on the melting curve are: the superfluid A transition T{sub A} = 2.505 mK, the A-B transition T{sub AB} = 1.948 mK, and the solid ordering temperature T{sub N} = 0.934 mK. The authors provide a functional form for P(T), which, with the fixed points, constitutes a convenient temperature scale, based on a primary thermometer, usable to well below 1 mK.

  11. Measurement of the top quark mass using the template method in the lepton plus jets channel with in situ W ---> j j calibration at CDF-II

    SciTech Connect

    Adelman, Jahred A.; Arguin, J.F.; Bellettini, G.; Brubaker, E.; Budagov, J.; Chlachidze, G.; Demortier, L.; Gibson, A.; Kim, S.; Kim, Y.K.; Maruyama, T.; Sato, K.; Shochet, M.; Sinervo, P.; Tomura, T.; Velev, G.; Xie, S.; Yang, U.K.; /Chicago U. /Toronto U. /INFN, Pisa /Dubna, JINR /Rockefeller U. /LBL, Berkeley /Tsukuba U. /Fermilab

    2006-05-01

    We report an updated measurement of the top quark mass in the lepton plus jets channel of t{bar t} events from p{bar p} collisions at {radical}s = 1.96 TeV. This measurement uses a dataset with integrated luminosity of 680 pb{sup -1}, containing 360 t{bar t} candidates separated into four subsamples. A top quark mass is reconstructed for each event by using energy and momentum constraints on the top quark pair decay products. We also employ the reconstructed mass of hadronic W boson decays W {yields} jj to constrain in situ the largest systematic uncertainty of the top quark mass measurement: the jet energy scale. Monte Carlo templates of the reconstructed top quark and W boson mass are produced as a function of the true top quark mass and the jet energy scale. The distribution of reconstructed top quark and W boson mass in the data are compared to the Monte Carlo templates using a likelihood fit to obtain: M{sub top} = 173.4 {+-} 2.8 GeV/c{sup 2}.

  12. CONSTRAINING THE PHYSICAL CONDITIONS IN THE JETS OF γ-RAY FLARING BLAZARS USING CENTIMETER-BAND POLARIMETRY AND RADIATIVE TRANSFER SIMULATIONS. II. EXPLORING PARAMETER SPACE AND IMPLICATIONS

    SciTech Connect

    Hughes, Philip A.; Aller, Margo F.; Aller, Hugh D. E-mail: mfa@umich.edu

    2015-02-01

    We analyze the shock-in-jet models for the γ-ray flaring blazars 0420-014, OJ 287, and 1156+295 presented in Paper I, quantifying how well the modeling constrains internal properties of the flow (low-energy spectral cutoff, partition between random and ordered magnetic field), the flow dynamics (quiescent flow speed and orientation), and the number and strength of the shocks responsible for radio-band flaring. We conclude that well-sampled, multifrequency polarized flux light curves are crucial for defining source properties. We argue for few, if any, low-energy particles in these flows, suggesting no entrainment and efficient energization of jet material, and for approximate energy equipartition between the random and ordered magnetic field components, suggesting that the ordered field is built by nontrivial dynamo action from the random component, or that the latter arises from a jet instability that preserves the larger-scale, ordered flow. We present evidence that the difference between orphan radio-band (no γ-ray counterpart) and non-orphan flares is due to more complex shock interactions in the latter case.

  13. Constraining the Physical Conditions in the Jets of γ-Ray Flaring Blazars Using Centimeter-band Polarimetry and Radiative Transfer Simulations. II. Exploring Parameter Space and Implications

    NASA Astrophysics Data System (ADS)

    Hughes, Philip A.; Aller, Margo F.; Aller, Hugh D.

    2015-02-01

    We analyze the shock-in-jet models for the γ-ray flaring blazars 0420-014, OJ 287, and 1156+295 presented in Paper I, quantifying how well the modeling constrains internal properties of the flow (low-energy spectral cutoff, partition between random and ordered magnetic field), the flow dynamics (quiescent flow speed and orientation), and the number and strength of the shocks responsible for radio-band flaring. We conclude that well-sampled, multifrequency polarized flux light curves are crucial for defining source properties. We argue for few, if any, low-energy particles in these flows, suggesting no entrainment and efficient energization of jet material, and for approximate energy equipartition between the random and ordered magnetic field components, suggesting that the ordered field is built by nontrivial dynamo action from the random component, or that the latter arises from a jet instability that preserves the larger-scale, ordered flow. We present evidence that the difference between orphan radio-band (no γ-ray counterpart) and non-orphan flares is due to more complex shock interactions in the latter case.

  14. Behavioural sensitisation to MK-801 is dose-dependent and independent of environmental context.

    PubMed

    Lefevre, Emilia M; Eyles, Darryl W; Burne, Thomas H J

    2016-02-01

    The pathophysiology of schizophrenia is associated with disturbed glutamate signalling, particularly via a dysfunction of the N-methyl-d-aspartate (NMDA) receptor. In rodents, behavioural sensitisation to the NMDA receptor antagonist MK-801 is proposed to recapitulate aspects of the NMDA receptor hypofunction hypothesis of schizophrenia. The aim of this study was to determine the modulatory role of MK-801 dose and environmental context on the development and expression of MK-801-induced behavioural sensitisation. Sprague Dawley rats were administered saline or varying doses of MK-801 (i.p.) once daily for 7 days and locomotor activity was recorded. After 5 days of withdrawal, rats were challenged with their respective dose to test for sensitisation. From this experiment a sensitising dose was obtained. In the second experiment the magnitude of sensitisation was compared between rats that were treated in either a home or test environmental context. Rats treated with 0.25mg/kg MK-801 developed robust sensitisation when challenged after withdrawal. Rats treated with lower (0.1mg/kg) or higher (0.5mg/kg) doses of MK-801 did not develop locomotor sensitisation. Sensitisation to 0.25mg/kg MK-801 developed equally between rats treated in the home or test context. The study shows that male Sprague Dawley rats develop behavioural sensitisation to repeated injections of MK-801. The development of sensitisation is selective to MK-801 dose in an inverted-U dose response fashion. In this paradigm MK-801 induced sensitisation was expressed similarly between groups treated in two distinct environmental contexts. PMID:26593108

  15. Normal cadmium uptake in microcytic anemia mk/mk mice suggests that DMT1 is not the only cadmium transporter in vivo

    SciTech Connect

    Suzuki, Tomohito; Momoi, Kanae; Hosoyamada, Makoto Kimura, Masaki; Shibasaki, Toshiaki

    2008-03-15

    Divalent metal transporter 1 (DMT1) is a mammalian iron (Fe) transporter and also transports Cadmium (Cd) in vitro. This study compared Cd absorption in DMT1-dysfunctional MK/Rej-{sup mk}/{sub mk} mice (mk/mk mice) and in DMT1-functional, Fe-deficient wild-type (WT) mice, to clarify the role of DMT1 in intestinal Cd absorption in vivo. Mice were given 1 ppm CdCl{sub 2} aq in drinking water for 2 weeks, and the concentrations of Cd and Fe in liver, kidney, and intestinal epithelium were subsequently determined. The Fe concentration in intestinal epithelia of WT mice was decreased in proportion to the level of dietary Fe limitation, while Cd accumulation under the same conditions was increased. DMT1 mRNA expression in the small intestine of Fe-deficient WT mice was clearly increased compared to that in Fe-sufficient WT mice. Iron deficiency resulted in up-regulation of Cd uptake in the intestine of Fe-deficient WT mice. The mk/mk mice have a mutation in DMT1 and loss of its function led to decreased intestinal Fe concentration. However, intestinal Cd accumulation was the same as in WT mice and it was also increased in Fe-deficient situation. There is the possibility that an unknown Cd pathway has taken a role on Cd intestinal absorption in vivo and that this pathway is regulated by food Fe concentrations. Therefore, DMT1 is not the sole transporter of intestinal cadmium absorption in vivo.

  16. Jet Dipolarity: Top Tagging with Color Flow

    SciTech Connect

    Hook, Anson; Jankowiak, Martin; Wacker, Jay G.; /SLAC

    2011-08-12

    A new jet observable, dipolarity, is introduced that can distinguish whether a pair of subjets arises from a color singlet source. This observable is incorporated into the HEPTopTagger and is shown to improve discrimination between top jets and QCD jets for moderate to high p{sub T}. The impressive resolution of the ATLAS and CMS detectors means that a typical QCD jet at the LHC deposits energy in {Omicron}(10-100) calorimeter cells. Such fine-grained calorimetry allows for jets to be studied in much greater detail than previously, with sophisticated versions of current techniques making it possible to measure more than just the bulk properties of jets (e.g. event jet multiplicities or jet masses). One goal of the LHC is to employ these techniques to extend the amount of information available from each jet, allowing for a broader probe of the properties of QCD. The past several years have seen significant progress in developing such jet substructure techniques. A number of general purpose tools have been developed, including: (i) top-tagging algorithms designed for use at both lower and higher p{sub T} as well as (ii) jet grooming techniques such as filtering, pruning, and trimming, which are designed to improve jet mass resolution. Jet substructure techniques have also been studied in the context of specific particle searches, where they have been shown to substantially extend the reach of traditional search techniques in a wide variety of scenarios, including for example boosted Higgses, neutral spin-one resonances, searches for supersymmetry, and many others. Despite these many successes, however, there is every reason to expect that there remains room for refinement of jet substructure techniques.

  17. The PIT MkV pulsed inductive thruster

    NASA Technical Reports Server (NTRS)

    Dailey, C. Lee; Lovberg, Ralph H.

    1993-01-01

    The pulsed inductive thruster (PIT) is an electrodeless, magnetic rocket engine that can operate with any gaseous propellant. A puff of gas injected against the face of a flat (spiral) coil is ionized and ejected by the magnetic field of a fast-rising current pulse from a capacitor bank discharge. Single shot operation on an impulse balance has provided efficiency and I(sub sp) data that characterize operation at any power level (pulse rate). The 1-m diameter MkV thruster concept offers low estimated engine mass at low powers, together with power capability up to more than 1 MW for the 1-m diameter design. A 20 kW design estimate indicates specific mass comparable to Ion Engine specific mass for 10,000 hour operation, while a 100,000 hour design would have a specific mass 1/3 that of the Ion Engine. Performance data are reported for ammonia and hydrazine. With ammonia, at 32 KV coil voltage, efficiency is a little more than 50 percent from 4000 to more than 8000 seconds I(sub sp). Comparison with data at 24 and 28 kV indicates that a wider I(sub sp) range could be achieved at higher coil voltages, if required for deep space missions.

  18. Identification of Statistical Invariance for Anodic Signals of Mk-IV Electrorefiner

    SciTech Connect

    Supathorn Phongikaroon; Tae-Sic Yoo

    2007-09-01

    A statistical invariance technique is proposed for an analysis of anodic signals from the Mk-IV electrorefiner (ER) currently used for treating spent EBR-II fuel. Voltage and applied current signals obtained from the Data Archival Software System (DASS) were used in this study. In general, the plots of these signals from different experimental runs present complex patterns to analyze—the currents were adjusted and shut-off due to limited ampere-hr or cut-off cell voltage; the voltage would increase showing a sign that uranium in the fuel elements had been depleted. Rather than directly analyzing these sets of time-series signals, a simple nonlinear function of these signal sequences and division were observed, which returned resistance series information. The primary idea deriving the methodology presented in this paper is that “anodic resistance time series should show intrinsic kinetic progress of anodic ER process.” A simple histogram-based analysis reveals notable statistical information, which may be invariant under ideal ER operating conditions. For instance, the results suggest that mostly uranium dissolution would be preferentially transferred around 0.00217 - 0.00354 ohm and other minor distribution peaks may possibly represent other transfers of fission species in the system.

  19. Decline in Age-dependent, MK801-induced Injury Coincides With Developmental Switch in Parvalbumin Expression: Somatosensory and Motor Cortex

    PubMed Central

    Tomé, Carla M Lema; Miller, Ryan; Bauer, Clayton; Smith, Chelsey; Blackstone, Kaitlin; Leigh, Adam; Busch, Jamie; Turner, Christopher P

    2009-01-01

    MK801-induced activation of caspase-3 is developmentally regulated, peaking at postnatal day (P) 7 and decreasing with increasing postnatal age thereafter. Further, at P7, cells displaying activation of caspase-3 lack expression of calcium binding proteins (CaBPs). To further explore this relationship, we investigated postnatal expression of calbindin (CB), calretinin (CR) and parvalbumin (PV) in two brain regions susceptible to MK801-induced injury, the somatosensory cortex (S1) and layer II/III of motor cortex (M1/M2). Expression of CB and especially PV was low to absent prior to P7 but substantially increased from P7 through to P21 and adulthood. In contrast, CR expression was more variable at early developmental ages, stabilized to lower levels after P7 and showed a marked decline by P21. The results suggest that not only does calcium buffering capacity increase developmentally but acquisition of enhanced buffering may be one mechanism by which neurons survive agent-induced alterations in calcium homeostasis. PMID:18688810

  20. The effect of high curing temperature on the reaction kinetics in MK/lime and MK-blended cement matrices at 60 deg. C

    SciTech Connect

    Rojas, Moises Frias; Sanchez de Rojas, M.I

    2003-05-01

    It is well known that the pozzolanic reaction between metakaolin (MK) and calcium hydroxide produces CSH, C{sub 2}ASH{sub 8} (stratlingite), C{sub 4}AH{sub 13} and C{sub 3}ASH{sub 6} (hydrogarnet). However, the presence or absence of these hydrated phases depends on different parameters, such as curing temperature, matrix used, etc. This paper shows the results of a study in order to know the effect of high curing temperature (60 deg. C) on the kinetics of the pozzolanic reaction in different matrices. MK/lime (calcium hydroxide) and MK-blended cement matrices were studied in samples stored and cured at 60 deg. C and up to 123 days of hydration. The nature, sequence and crystallinity of the hydrated phases were analysed using differential thermal analysis (DTA) and X-ray diffraction (XRD) techniques. Results showed that the sequence and formation of the hydrated phases was different in both matrices cured at 60 deg. C. In an MK/lime matrix, C{sub 2}ASH{sub 8}, C{sub 4}AH{sub 13} and C{sub 3}ASH{sub 6} were the main hydrated phases; while in an MK-blended cement, stratlingite was the sole hydrated phase issued from pozzolanic reaction. The DTA and XRD data also reveal an important fact: there is no evidence of the presence of hydrogarnet in blended cements.

  1. New insights into the activation, interaction partners and possible functions of MK5/PRAK.

    PubMed

    Perander, Maria; Keyse, Stephen M; Seternes, Ole-Morten

    2016-01-01

    MAP kinase-activated protein kinase 5 (MK5) was first described as a downstream target of the p38 MAP kinase pathway leading to its alternative acronym of p38-regulated/activated protein kinase (PRAK). However, since the discovery that MK5 is a bona fide interaction partner of the atypical MAP kinases ERK3 and ERK4 and that this interaction leads to both the activation and subcellular relocalisation of MK5, there has been considerable debate as to the relative roles of these MAPK pathways in mediating the activation and biological functions of MK5. Here we discuss recent progress in defining novel upstream components of the ERK3/ERK4 signalling pathway, our increased understanding of the mechanism by which MK5 interacts with and is activated by ERK3 and ERK4, and the discovery of novel interaction partners for MK5. Finally, we review recent literature that suggests novel biological functions for MK5 in a range of physiological and pathophysiological conditions including neuronal function and cancer. PMID:26709779

  2. Differential Effects of Olanzapine and Haloperidol on MK-801-induced Memory Impairment in Mice

    PubMed Central

    Song, Jae Chun; Seo, Mi Kyoung; Park, Sung Woo; Lee, Jung Goo; Kim, Young Hoon

    2016-01-01

    Objective We investigated the differential effects of the antipsychotic drugs olanzapine and haloperidol on MK-801-induced memory impairment and neurogenesis in mice. Methods MK-801 (0.1 mg/kg) was administered 20 minutes prior to behavioral testing over 9 days. Beginning on the sixth day of MK-801 treatment, either olanzapine (0.05 mg/kg) or haloperidol (0.05 mg/kg) was administered 40 minutes prior to MK-801 for the final 4 days. Spatial memory performance was measured using a Morris water maze (MWM) test for 9 days (four trials/day). Immunohistochemistry with bromodeoxyuridine (BrdU) was used to identify newborn cells labeled in tissue sections from the dentate gyrus of the hippocampus. Results MK-801 administration over 9 days significantly impaired memory performance in the MWM test compared to untreated controls (p<0.05) and these deficits were blocked by treatment with olanzapine (p<0.05) but not haloperidol. The administration of MK-801 also resulted in a decrease in the number of BrdU-labeled cells in the dentate gyrus (28.6%; p<0.01), which was prevented by treatment with olanzapine (p<0.05) but not haloperidol. Conclusion These results suggest that olanzapine has a protective effect against cognitive impairments induced by MK-801 in mice via the stimulating effects of neurogenesis. PMID:27489382

  3. MK2: an unrecognized regulator of tumor promoting macrophages in colorectal cancer?

    PubMed Central

    Castillo, Eliseo F.; Ray, Anita L.; Beswick, Ellen J.

    2016-01-01

    Colorectal cancer (CRC) is one of the most common malignancies and is associated closely with inflammation before and after development. Macrophages promote colitis and colitis-associated CRC. M1 macrophages contribute to colitis directly through the production of proinflammatory cytokines and through activation of proinflammatory immune cell phenotypes. In cancer, both M1 and M2 macrophages participate in tumor development and progression through cytokine production, changes in cell signaling and activation of T cells. We have identified the mitogen-activated protein kinase-activated protein kinase 2 (MK2) as a regulator of macrophages during colitis-associated CRC (CAC). MK2 is a proinflammatory kinase that promotes production of IL-1α, IL-1β, IL-6 and TNF-α. MK2−/− mice have decreases in macrophages, macrophage-associated chemokines, and proinflammatory cytokines. Most significantly, MK2−/− mice do not develop neoplasms in an inflammatory model of CRC. However, addition of MK2+/+ macrophages to MK2−/− mice increases production of proinflammatory cytokines. In wild type mice, both cytokines and tumor burdens increase upon addition of additional macrophages. These data support the importance of MK2 in macrophage regulation during inflammation-associated CRC. PMID:26998523

  4. Interaction of the NMDA receptor noncompetitive antagonist MK-801 with model and native membranes.

    PubMed Central

    Moring, J; Niego, L A; Ganley, L M; Trumbore, M W; Herbette, L G

    1994-01-01

    MK-801, a noncompetitive antagonist of the NMDA (N-methyl-D-aspartate) receptor, has protective effects against excitotoxicity and ethanol withdrawal seizures. We have determined membrane/buffer partition coefficients (Kp[mem]) of MK-801 and its rates of association with and dissociation from membranes. Kp[mem] (+/- SD) = 1137 (+/- 320) in DOPC membranes and 485 (+/- 99) in synaptoneurosomal (SNM) lipid membranes from rat cerebral cortex (unilamellar vesicles). In multilamellar vesicles, Kp[mem] was higher: 3374 (+/- 253) in DOPC and 6879 (+/- 947) in SNM. In cholesterol/DOPC membranes, Kp[mem] decreased as the cholesterol content increased. MK-801 associated with and dissociated from membranes rapidly. Addition of ethanol to SNM did not affect Kp[mem]. MK-801 decreased the cooperative unit size of DMPC membranes. The decrease was smaller than that caused by 1,4-dihydropyridine drugs, indicating a weaker interaction with the hydrocarbon core. Small angle x-ray diffraction, with multilayer autocorrelation difference function modeling, indicated that MK-801 in a cholesterol/DOPC membrane (mole ratio = 0.6) causes a perturbation at approximately 16.0 A from the bilayer center. In bilayers of cholesterol/DOPC = 0.15 (mole ratio) or pure DOPC, the perturbation caused by MK-801 was more complex. The physical chemical interactions of MK-801 with membranes in vitro are consistent with a fast onset and short duration of action in vivo. PMID:7696477

  5. Reactive Transformation and Increased BDNF Signaling by Hippocampal Astrocytes in Response to MK-801

    PubMed Central

    Wang, Yueming; Li, Guanjun; Wang, Lihua; Li, Huafang

    2015-01-01

    MK-801, also known as dizocilpine, is a noncompetitive N-methyl-D-aspartic acid (NMDA) receptor antagonist that induces schizophrenia-like symptoms. While astrocytes have been implicated in the pathophysiology of psychiatric disorders, including schizophrenia, astrocytic responses to MK-801 and their significance to schizotypic symptoms are unclear. Changes in the expression levels of glial fibrillary acid protein (GFAP), a marker of astrocyte activation in response to a variety of pathogenic stimuli, were examined in the hippocampus of rats treated with the repeated MK-801 injection (0.5 mg/10ml/kg body weight for 6 days) and in primary cultured hippocampal astrocytes incubated with MK-801 (5 or 20 μM for 24 h). Moreover, the expression levels of BDNF and its receptors TrkB and p75 were examined in MK-801-treated astrocyte cultures. MK-801 treatment enhanced GFAP expression in the rat hippocampus and also increased the levels of GFAP protein and mRNA in hippocampal astrocytes in vitro. Treatment of cultured hippocampal astrocytes with MK-801 enhanced protein and mRNA levels of BDNF, TrkB, and p75. Collectively, our results suggest that hippocampal astrocytes may contribute to the pathophysiology of schizophrenia symptoms associated with NMDA receptor hypofunction by reactive transformation and altered BDNF signaling. PMID:26700309

  6. Effects of MK-467 on the antinociceptive and sedative actions and pharmacokinetics of medetomidine in dogs.

    PubMed

    Bennett, R C; Salla, K M; Raekallio, M R; Hänninen, L; Rinne, V M; Scheinin, M; Vainio, O M

    2016-08-01

    We investigated the influence of the peripherally acting α2 -adrenoceptor antagonist MK-467 on the sedative and antinociceptive actions and plasma drug concentrations of medetomidine, an α2 -adrenoceptor agonist that is used in veterinary medicine as a sedative and analgesic agent. Eight healthy beagle dogs received intravenous medetomidine (10 μg/kg) or medetomidine with MK-467 (250 μg/kg) in a randomized crossover design. A standardized nociceptive pressure stimulus was applied to a nail bed of a hindlimb. Times for withdrawal of the limb and for head lift were measured, and sedation was scored. EEG data were collected prior to and after stimulation. Plasma drug concentrations were measured. Co-administration of MK-467 significantly attenuated medetomidine analgesia, as assessed with limb withdrawal, and also shortened the duration of sedation. The apparent plasma clearance of both enantiomers of medetomidine, dexmedetomidine and levomedetomidine, was more than doubled in the presence of MK-467. Antagonism by MK-467 of medetomidine-evoked vasoconstriction is seen as the mechanism behind this pharmacokinetic drug interaction. Thus, MK-467 attenuated the antinociceptive and sedative effects of medetomidine. This can probably be explained by increased clearance and decreased concentrations of dexmedetomidine in plasma after co-administration of MK-467 with racemic medetomidine. PMID:26763145

  7. NAAG peptidase inhibitors block cognitive deficit induced by MK-801 and motor activation induced by d-amphetamine in animal models of schizophrenia.

    PubMed

    Olszewski, R T; Janczura, K J; Ball, S R; Madore, J C; Lavin, K M; Lee, J C-M; Lee, M J; Der, E K; Hark, T J; Farago, P R; Profaci, C P; Bzdega, T; Neale, J H

    2012-01-01

    The most widely validated animal models of the positive, negative and cognitive symptoms of schizophrenia involve administration of d-amphetamine or the open channel NMDA receptor blockers, dizocilpine (MK-801), phencyclidine (PCP) and ketamine. The drug ZJ43 potently inhibits glutamate carboxypeptidase II (GCPII), an enzyme that inactivates the peptide transmitter N-acetylaspartylglutamate (NAAG) and reduces positive and negative behaviors induced by PCP in several of these models. NAAG is an agonist at the metabotropic glutamate receptor 3 (mGluR3). Polymorphisms in this receptor have been associated with expression of schizophrenia. This study aimed to determine whether two different NAAG peptidase inhibitors are effective in dopamine models, whether their efficacy was eliminated in GCPII knockout mice and whether the efficacy of these inhibitors extended to MK-801-induced cognitive deficits as assessed using the novel object recognition test. ZJ43 blocked motor activation when given before or after d-amphetamine treatment. (R,S)-2-phosphono-methylpentanedioic acid (2-PMPA), another potent NAAG peptidase inhibitor, also reduced motor activation induced by PCP or d-amphetamine. 2-PMPA was not effective in GCPII knockout mice. ZJ43 and 2-PMPA also blocked MK-801-induced deficits in novel object recognition when given before, but not after, the acquisition trial. The group II mGluR antagonist LY341495 blocked the effects of NAAG peptidase inhibition in these studies. 2-PMPA was more potent than ZJ43 in a test of NAAG peptidase inhibition in vivo. By bridging the dopamine and glutamate theories of schizophrenia with two structurally different NAAG peptidase inhibitors and demonstrating their efficacy in blocking MK-801-induced memory deficits, these data advance the concept that NAAG peptidase inhibition represents a potentially novel antipsychotic therapy. PMID:22850437

  8. In Vitro Characterization of MK-1439, a Novel HIV-1 Nonnucleoside Reverse Transcriptase Inhibitor

    PubMed Central

    Feng, Meizhen; Falgueyret, Jean-Pierre; Tawa, Paul; Witmer, Marc; DiStefano, Daniel; Li, Yuan; Burch, Jason; Sachs, Nancy; Lu, Meiqing; Cauchon, Elizabeth; Campeau, Louis-Charles; Grobler, Jay; Yan, Youwei; Ducharme, Yves; Côté, Bernard; Asante-Appiah, Ernest; Hazuda, Daria J.; Miller, Michael D.

    2014-01-01

    Nonnucleoside reverse transcriptase inhibitors (NNRTIs) are a mainstay of therapy for treating human immunodeficiency type 1 virus (HIV-1)-infected patients. MK-1439 is a novel NNRTI with a 50% inhibitory concentration (IC50) of 12, 9.7, and 9.7 nM against the wild type (WT) and K103N and Y181C reverse transcriptase (RT) mutants, respectively, in a biochemical assay. Selectivity and cytotoxicity studies confirmed that MK-1439 is a highly specific NNRTI with minimum off-target activities. In the presence of 50% normal human serum (NHS), MK-1439 showed excellent potency in suppressing the replication of WT virus, with a 95% effective concentration (EC95) of 20 nM, as well as K103N, Y181C, and K103N/Y181C mutant viruses with EC95 of 43, 27, and 55 nM, respectively. MK-1439 exhibited similar antiviral activities against 10 different HIV-1 subtype viruses (a total of 93 viruses). In addition, the susceptibility of a broader array of clinical NNRTI-associated mutant viruses (a total of 96 viruses) to MK-1439 and other benchmark NNRTIs was investigated. The results showed that the mutant profile of MK-1439 was superior overall to that of efavirenz (EFV) and comparable to that of etravirine (ETR) and rilpivirine (RPV). Furthermore, E138K, Y181C, and K101E mutant viruses that are associated with ETR and RPV were susceptible to MK-1439 with a fold change (FC) of <3. A two-drug in vitro combination study indicated that MK-1439 acts nonantagonistically in the antiviral activity with each of 18 FDA-licensed drugs for HIV infection. Taken together, these in vitro data suggest that MK-1439 possesses the desired properties for further development as a new antiviral agent. PMID:24379202

  9. Jets in air-jet family

    NASA Technical Reports Server (NTRS)

    Navia, C. E.; Sawayanagi, K.

    1985-01-01

    The A-jet families on Chacaltaya emulsion chamber experiments were analyzed by the study of jets which are reconstructed by a grouping procedure. It is demonstrated that large-E sub J R sub J events are characterized by small number of jets and two-jet like asymmetric shape, binocular events and the other type. This type has a larger number of jets and more symmetrical shape in the P sub t plane.

  10. Marine Jet

    NASA Technical Reports Server (NTRS)

    1978-01-01

    The marine turbine pump pictured is the Jacuzzi 12YJ, a jet propulsion system for pleasure or commercial boating. Its development was aided by a NASA computer program made available by the Computer Software Management and Information Center (COSMIC) at the University of Georgia. The manufacturer, Jacuzzi Brothers, Incorporated, Little Rock, Arkansas, used COSMIC'S Computer Program for Predicting Turbopump Inducer Loading, which enabled substantial savings in development time and money through reduction of repetitive testing.

  11. Weak-value thermostat with 0.2 mK precision.

    PubMed

    Egan, Patrick; Stone, Jack A

    2012-12-01

    A new laser-based thermostat sensitive to 0.2 mK at room temperature is reported. The method utilizes a fluid-filled prism and interferometric weak-value amplification to sense nanoradian deviations of a laser beam: due to the high thermo-optic coefficient of the fluid (colorless fluorocarbon), the deviation angle through the prism is sensitive to temperature. We estimate the daily stability of our device to be 0.2 mK, which is limited by drifts in the apparatus, and the narrow 20 mK capture range is the price paid for the weak measurement. PMID:23202114

  12. The role of purinergic and dopaminergic systems on MK-801-induced antidepressant effects in zebrafish.

    PubMed

    da Silva, Raquel Bohrer; Siebel, Anna Maria; Bonan, Carla Denise

    2015-12-01

    Depression is a serious disease characterized by low mood, anhedonia, loss of interest in daily activities, appetite and sleep disturbances, reduced concentration, and psychomotor agitation. There is a growing interest in NMDA antagonists as a promising target for the development of new antidepressants. Considering that purinergic and dopaminergic systems are involved in depression and anxiety states, we characterized the role of these signaling pathways on MK-801-induced antidepressant effects in zebrafish. Animals treated with MK-801 at the doses of 5, 10, 15, or 20μM during 15, 30, or 60min spent longer time in the top area of aquariums in comparison to control group, indicating an anxiolytic/antidepressant effect induced by this drug. Animals treated with MK-801 spent longer time period at top area until 2 (5μM MK-801) and 4 (20μM MK-801) hours after treatment, returning to basal levels from 24h to 7days after exposure. Repeated MK-801 treatment did not induce cumulative effects, since animals treated daily during 7days had the same behavioral response pattern observed since the first until the 7th day. In order to investigate the effects of adenosine A1 and A2A receptor antagonist and agonist and the influence of modulation of adenosine levels on MK-801 effects, we treated zebrafish with caffeine, DPCPX, CPA, ZM 241385, CGS 21680, AMPCP, EHNA, dipyridamole, and NBTI during 30min before MK-801 exposure. The non-specific adenosine receptor antagonist caffeine (50mg/kg) and the selective A1 receptor antagonist DPCPX (15mg/kg) prevented the behavioral changes induced by MK-801. The non-specific nucleoside transporter (NT) inhibitor dipyridamole (10mg/kg) exacerbated the behavioral changes induced by MK-801. Dopamine receptor antagonists (sulpiride and SCH 23390) did not change the behavioral alterations induced by MK-801. Our findings demonstrated that antidepressant-like effects of MK-801 in zebrafish are mediated through adenosine A1 receptor activation. PMID

  13. Filament Eruptions, Jets, and Space Weather

    NASA Technical Reports Server (NTRS)

    Moore, Ronald; Sterling, Alphonse; Robe, Nick; Falconer, David; Cirtain, Jonathan

    2013-01-01

    Previously, from chromospheric H alpha and coronal X-ray movies of the Sun's polar coronal holes, it was found that nearly all coronal jets (greater than 90%) are one or the other of two roughly equally common different kinds, different in how they erupt: standard jets and blowout jets (Yamauchi et al 2004, Apl, 605, 5ll: Moore et all 2010, Apj, 720, 757). Here, from inspection of SDO/AIA He II 304 A movies of 54 polar x-ray jets observed in Hinode/XRT movies, we report, as Moore et al (2010) anticipated, that (1) most standard x-ray jets (greater than 80%) show no ejected plasma that is cool enough (T is less than or approximately 10(exp 5K) to be seen in the He II 304 A movies; (2) nearly all blownout X-ray jets (greater than 90%) show obvious ejection of such cool plasma; (3) whereas when cool plasma is ejected in standard X-ray jets, it shows no lateral expansion, the cool plasma ejected in blowout X-ray jets shows strong lateral expansion; and (4) in many blowout X-ray jets, the cool plasma ejection displays the erupting-magnetic-rope form of clasic filament eruptions and is thereby seen to be a miniature filament eruption. The XRT movies also showed most blowout X-ray jets to be larger and brighter, and hence to apparently have more energy, than most standard X-ray jets. These observations (1) confirm the dichotomy of coronal jets, (2) agree with the Shibata model for standard jets, and (3) support the conclusion of Moore et al (2010) that in blowout jets the magnetic-arch base of the jet erupts in the manner of the much larger magnetic arcades in which the core field, the field rooted along the arcade's polarity inversion line, is sheared and twisted (sigmoid), often carries a cool-plasma filament, and erupts to blowout the arcade, producing a CME. From Hinode/SOT Ca II movies of the polar limb, Sterling et al (2010, ApJ, 714, L1) found that chromospheric Type-II spicules show a dichotomy of eruption dynamics similar to that found here for the cool

  14. DAG/PKCδ and IP3/Ca2+/CaMK IIβ Operate in Parallel to Each Other in PLCγ1-Driven Cell Proliferation and Migration of Human Gastric Adenocarcinoma Cells, through Akt/mTOR/S6 Pathway

    PubMed Central

    Dai, Lianzhi; Zhuang, Luhua; Zhang, Bingchang; Wang, Fen; Chen, Xiaolei; Xia, Chun; Zhang, Bing

    2015-01-01

    Phosphoinositide specific phospholipase Cγ (PLCγ) activates diacylglycerol (DAG)/protein kinase C (PKC) and inositol 1,4,5-trisphosphate (IP3)/Ca2+/calmodulin-dependent protein kinase II (CaMK II) axes to regulate import events in some cancer cells, including gastric adenocarcinoma cells. However, whether DAG/PKCδ and IP3/Ca2+/CaMK IIβ axes are simultaneously involved in PLCγ1-driven cell proliferation and migration of human gastric adenocarcinoma cells and the underlying mechanism are not elucidated. Here, we investigated the role of DAG/PKCδ or CaMK IIβ in PLCγ1-driven cell proliferation and migration of human gastric adenocarcinoma cells, using the BGC-823 cell line. The results indicated that the inhibition of PKCδ and CaMK IIβ could block cell proliferation and migration of BGC-823 cells as well as the effect of inhibiting PLCγ1, including the decrease of cell viability, the increase of apoptotic index, the down-regulation of matrix metalloproteinase (MMP) 9 expression level, and the decrease of cell migration rate. Both DAG/PKCδ and CaMK IIβ triggered protein kinase B (Akt)/mammalian target of rapamycin (mTOR)/S6 pathway to regulate protein synthesis. The data indicate that DAG/PKCδ and IP3/Ca2+/CaMK IIβ operate in parallel to each other in PLCγ1-driven cell proliferation and migration of human gastric adenocarcinoma cells through Akt/mTOR/S6 pathway, with important implication for validating PLCγ1 as a molecular biomarker in early gastric cancer diagnosis and disease surveillance. PMID:26633375

  15. Top Jets at the LHC

    SciTech Connect

    Almeida, L.G.; Lee, S.J.; Perez, G.; Sung, I.; Virzi, J.

    2008-10-06

    We investigatethe reconstruction of high pT hadronically-decaying top quarksat the Large Hadron Collider. One of the main challenges in identifying energetictop quarks is that the decay products become increasingly collimated. This reducesthe efficacy of conventional reconstruction methods that exploit the topology of thetop quark decay chain. We focus on the cases where the decay products of the topquark are reconstructed as a single jet, a"top-jet." The most basic"top-tag" methodbased on jet mass measurement is considered in detail. To analyze the feasibility ofthe top-tagging method, both theoretical and experimental aspects of the large QCDjet background contribution are examined. Based on a factorization approach, wederive a simple analytic approximation for the shape of the QCD jet mass spectrum.We observe very good agreement with the Monte Carlo simulation. We consider high pT tt bar production in the Standard Model as an example, and show that our theoretical QCD jet mass distributions can efficiently characterize the background via sideband analyses. We show that with 25 fb-1 of data, our approach allows us to resolve top-jets with pT _> 1 TeV, from the QCD background, and about 1.5 TeV top-jets with 100 fb-1, without relying on b-tagging. To further improve the significancewe consider jet shapes (recently analyzed in 0807.0234 [hep-ph]), which resolve thesubstructure of energy flow inside cone jets. A method of measuring the top quarkpolarization by using the transverse momentum of the bottom quark is also presented.The main advantages of our approach are: (i) the mass distributions are driven byfirst principle calculations, instead of relying solely on Monte Carlo simulation; (ii) for high pT jets (pT _> 1 TeV), IR-safe jet shape variables are robust against detectorresolution effects. Our analysis can be applied to other boosted massive particlessuch as the electroweak gauge bosons and the Higgs.

  16. Rapid single-flux-quantum circuits for low noise mK operation

    NASA Astrophysics Data System (ADS)

    Intiso, Samuel; Pekola, Jukka; Savin, Alexander; Devyatov, Ygor; Kidiyarova-Shevchenko, Anna

    2006-05-01

    Rapid single-flux-quantum (RSFQ) technology has been proposed as control electronics for superconducting quantum bits because of the material and working temperature compatibility. In this work, we consider practical aspects of RSFQ circuit design for low noise low power operation. At the working temperature of 20 mK and operational frequency of 2 GHz, dissipated power per junction is reduced to 25 pW by using 6 µA critical current junctions available at the Hypres and VTT low Jc fabrication process. To limit phonon temperature to 30 mK, a maximum of 40 junctions can be placed on a 5 mm × 5 mm chip. Electron temperature in resistive shunts of Josephson junctions is minimized by use of cooling fins, giving minimum electron temperatures of about 150 mK for the Hypres process and 70 mK for the VTT process.

  17. Mechanism of action of MK-401 against Fasciola hepatica: inhibition of phosphoglycerate kinase.

    PubMed

    Schulman, M D; Ostlind, D A; Valentino, D

    1982-03-01

    The effect of MK-401 (4-amino-6-trichloroethenyl 1,3-benzenedisulfonamide) on Fasciola hepatica phosphoglycerate kinase (EC 2.7.2.3) was investigated. MK-401 was a competitive inhibitor of both 3-phosphoglycerate and ATP and had a Ki of 0.29 mM. ATP, 1,3-diphosphoglycerate and MK-401 protected the Fasciola enzyme from inhibition by N-ethylmaleimide. Analogues of MK-401 with different substituents at the 6 position (R = Cl, CF3, C2 F3, C3 F7) were competitive inhibitors of both 3-phosphoglycerate and ATP and a good correlation between the Ki and in vivo activity of these analogues was observed. PMID:7088033

  18. Pyrroloquinoline quinone prevents MK-801-induced stereotypical behavior and cognitive deficits in mice.

    PubMed

    Zhou, Xingqin; Chen, Quancheng; Hu, Xindai; Mao, Shishi; Kong, Yanyan

    2014-01-01

    Pyrroloquinoline quinone (PQQ), an essential nutrient, antioxidant, redox modulator, and nerve growth factor, prevents cognitive deficits associated with oxidative stress-induced neurodegeneration. Previous molecular imaging studies also demonstrate that PQQ binds to N-methyl D-aspartate (NMDA) receptors. In this study, we investigated the effects of PQQ on stereotypical behaviors and cognitive deficits induced by MK-801, a non-competitive NMDA antagonist used to model schizophrenia. Mice were given repeated injections of MK-801 (0.5mg/kg/d) and PQQ (0.2, 2.0, or 20 μg/kg/d) for 60 days. Behavior was evaluated using a variety of motor, social, and cognitive tests. We found that PQQ administration significantly attenuated MK-801-induced increases in stereotypical behavior and ataxia, suggesting a protective role of PQQ against MK-801-induced neuronal dysfunction and psychiatric disorders. Future studies are necessary to elucidate the underlying mechanisms of PQQ. PMID:24149067

  19. Altitude Test Chamber Investigation of Performance of a 28-inch Ram-jet Engine II : Effects of Gutter Width and Blocked Area on Operating Range and Combustion Efficiency

    NASA Technical Reports Server (NTRS)

    Shillito, T B; Jones, W L; Kahn, R W

    1950-01-01

    Altitude-test-chamber investigation of effects of flame-holder blocked area and gutter width on performance of 28-inch diameter ram jet at simulated flight Mach number of 2.0 for altitudes from 40,000 to 55,000 feet was conducted at NACA Lewis laboratory. Ten flame holders investigated covered gutter widths from 1.00 to 2.50 inches and blocked areas from 40.5 to 62.0 percent of combustion-chamber area. Gutter width did not appreciably affect combustion efficiency. Increase in blocked area from 40 to 62 percent resulted in 5- to 10-percent increase in combustion efficiency. Increasing gutter width resulted in improvement in fuel-air-ratio operating range.

  20. The effect of ghrelin on MK-801 induced memory impairment in rats.

    PubMed

    Goshadrou, Fatemeh; Kermani, Mojtaba; Ronaghi, Abdolaziz; Sajjadi, Samad

    2013-06-01

    Accumulating evidence indicates that the brain-gut peptide ghrelin which is expressed in hippocampus improves memory and learning processes. The MK-801, a noncompetitive NMDA receptor antagonist, has also shown amnesic properties in animal model. The current study was to find out whether intracerebroventricular administration of ghrelin can prevent amnesia induced by MK-801 in rats. A week after the surgery, during which cannuals were implanted in the lateral ventricular, the animals were trained and tested in a step-through type passive avoidance task. Memory retrieval was measured by step-through latency (STL) and total time in dark compartments (TDC). In the first series of experiments, we established a dose-response relationship for ghrelin on the passive avoidance paradigm. In the second set of experiments, animals were divided to two groups. In the first group, MK-801 (0.075, 0.15 and 0.3mg/kg) was injected intraperitoneally (i.p.) immediately after the acquisition session and in the second group MK-801 (same doses) was injected (i.p.) 30 min before the retention session. Analysis of data showed that in both groups, MK-801 impaired learning and memory. In the third set of experiments, administration of ghrelin (200 ng/rat) right after the acquisition session (i.e. before MK-801 injection) improved the MK-801 induced memory impairment, but administration of ghrelin before retrieval session did not affect the MK-801 induced memory impairment. These results show an interaction between ghrelin and glutamatergic system. A novel finding in this study is that ghrelin can prevent amnesia produced by NMDA antagonist in rats when injected in post-training phase. PMID:23538209

  1. Cholinesterase inhibitors ameliorate behavioral deficits induced by MK-801 in mice.

    PubMed

    Csernansky, John G; Martin, Maureen; Shah, Renu; Bertchume, Amy; Colvin, Jenny; Dong, Hongxin

    2005-12-01

    Enhancing cholinergic function has been suggested as a possible strategy for ameliorating the cognitive deficits of schizophrenia. The purpose of this study was to examine the effects of acetylcholinesterase (AChE) inhibitors in mice treated with the noncompetitive N-methyl-d-aspartate (NMDA) receptor antagonist, MK-801, which has been suggested as an animal model of the cognitive deficits of schizophrenia. Three separate experiments were conducted to test the effects of physostigmine, donepezil, or galantamine on deficits in learning and memory induced by MK-801. In each experiment, MK-801 (0.05 or 0.10 mg/kg) or saline was administered i.p. 20 min prior to behavioral testing over a total of 12 days. At 30 min prior to administration of MK-801 or saline, one of three doses of the AChE inhibitor (ie physostigmine-0.03, 0.10, or 0.30 mg/kg; donepezil-0.10, 0.30, or 1.00 mg/kg; or galantamine-0.25, 0.50, or 1.00 mg/kg) or saline was administered s.c. Behavioral testing was performed in all experimental animals using the following sequence: (1) spatial reversal learning, (2) locomotion, (3) fear conditioning, and (4) shock sensitivity. Both doses of MK-801 produced impairments in spatial reversal learning and in contextual and cued memory, as well as hyperlocomotion. Physostigmine and donepezil, but not galantamine, ameliorated MK-801-induced deficits in spatial reversal learning and in contextual and cued memory in a dose-dependent manner. Also, physostigmine, but not donepezil or galantamine, reversed MK-801-induced hyperlocomotion. Galantamine, but not physostigmine or donepezil, altered shock sensitivity. These results suggest that AChE inhibitors may differ in their capacity to ameliorate learning and memory deficits produced by MK-801 in mice, which may have relevance for the cognitive effects of cholinomimetic drugs in patients with schizophrenia. PMID:15956997

  2. Identification of peptides in wheat germ hydrolysate that demonstrate calmodulin-dependent protein kinase II inhibitory activity.

    PubMed

    Kumrungsee, Thanutchaporn; Akiyama, Sayaka; Guo, Jian; Tanaka, Mitsuru; Matsui, Toshiro

    2016-12-15

    Hydrolysis of wheat germ by proteases resulted in bioactive peptides that demonstrated an inhibitory effect against the vasoconstrictive Ca(2+)-calmodulin (CaM)-dependent protein kinase II (CaMK II). The hydrolysate by thermolysin (1.0wt%, 5h) showed a particularly potent CaMK II inhibition. As a result of mixed mode high-performance liquid chromatography of thermolysin hydrolysate with pH elution gradient ranging between 4.8 and 8.9, the fraction eluted at pH 8.9 was the most potent CaMK II inhibitor. From this fraction, Trp-Val and Trp-Ile were identified as CaMK II inhibitors. In Sprague-Dawley rats, an enhanced aortic CaMK II activity by 1μM phenylephrine was significantly (p<0.05) suppressed by 15-min incubation with 300μM Trp-Val or Trp-Ile. On the basis of Ca(2+)-chelating fluorescence and CaMK II activity assays, it was concluded that Trp-Val and Trp-Ile competed with Ca(2+)-CaM complex to bind to CaMK II with Ki values of 5.4 and 3.6μM, respectively. PMID:27451188

  3. Jet Propulsion Laboratory/NASA Lewis Research Center space qualified hybrid high temperature superconducting/semiconducting 7.4 GHz low-noise downconverter for NRL HTSSE-II program

    SciTech Connect

    Javadi, H.H.S.; Bowen, J.G.; Rascoe, D.L.; Romanofsky, R.R.; Bhasin, K.B.; Chorey, C.M.

    1996-07-01

    A deep space satellite downconverter receiver was proposed by Jet Propulsion Laboratory (JPL) and NASA Lewis Research Center (LeRC) for the Naval Research Laboratory`s (NRL) high temperature superconductivity space experiment, phase-II (HTSSE-II) program. Space qualified low-noise cryogenic downconverter receivers utilizing thin-film high temperature superconducting (HTS) passive circuitry and semiconductor active devices were developed and delivered to NRL. The downconverter consists of an HTS preselect filter, a cryogenic low-noise amplifier, a cryogenic mixer, and a cryogenic oscillator with an HTS resonator. HTS components were inserted as the front-end filter and the local oscillator resonator for their superior 77 K performance over the conventional components. The semiconducting low noise amplifier also benefited from cooling to 77 K. The mixer was designed specifically for cryogenic applications and provided low conversion loss and low power consumption. In addition to an engineering model, two space qualified units (qualification, flight) were built and delivered to NRL. Manufacturing, integration and test of the space qualified downconverters adhered to the requirements of JPL class-D space instruments and partially to MIL-STD-883D specifications. The qualification unit has {approximately}50 K system noise temperature which is a factor of three better than a conventional downconverter at room temperature.

  4. Magnetic Untwisting in Most Solar X-Ray Jets

    NASA Technical Reports Server (NTRS)

    Moore, Ronald; Sterling, Alphonse; Falconer, David; Robe, Dominic

    2013-01-01

    From 54 X-ray jets observed in the polar coronal holes by Hinode's X-Ray Telescope (XRT) during coverage in movies from Solar Dynamic Observatory's Atmospheric Imaging Assembly (AIA) taken in its He II 304 Å band at a cadence of 12 s, we have established a basic characteristic of solar X-ray jets: untwisting motion in the spire. In this presentation, we show the progression of few of these X-ray jets in XRT images and track their untwisting in AIA He II images. From their structure displayed in their XRT movies, 19 jets were evidently standard jets made by interchange reconnection of the magnetic-arcade base with ambient open field, 32 were evidently blowout jets made by blowout eruption of the base arcade, and 3 were of ambiguous form. As was anticipated from the >10,000 km span of the base arcade in most polar X-ray jets and from the disparity of standard jets and blowout jets in their magnetic production, few of the standard X-ray jets (3 of 19) but nearly all of the blowout X-ray jets (29 of 32) carried enough cool (T is approximately 105 K) plasma to be seen in their He II movies. In the 32 X-ray jets that showed a cool component, the He II movies show 10-100 km/s untwisting motions about the axis of the spire in all 3 standard jets and in 26 of the 29 blowout jets. Evidently, the open magnetic field in nearly all blowout X-ray jets and probably in most standard X-ray jets carries transient twist. This twist apparently relaxes by propagating out along the open field as a torsional wave. High-resolution spectrograms and Dopplergrams have shown that most Type-II spicules have torsional motions of 10-30 km/s. Our observation of similar torsional motion in X-ray jets strengthens the case for Type-II spicules being made in the same way as X-ray jets, by blowout eruption of a twisted magnetic arcade in the spicule base and/or by interchange reconnection of the twisted base arcade with the ambient open field. This work was funded by NASA's Heliophysics Division

  5. The quiet Sun average Doppler shift of coronal lines up to 2 MK

    NASA Astrophysics Data System (ADS)

    Dadashi, N.; Teriaca, L.; Solanki, S. K.

    2011-10-01

    Context. The average Doppler shift shown by spectral lines formed from the chromosphere to the corona reveals important information on the mass and energy balance of the solar atmosphere, providing an important observational constraint to any models of the solar corona. Previous spectroscopic observations of vacuum ultra-violet (VUV) lines have revealed a persistent average wavelength shift of lines formed at temperatures up to 1 MK. At higher temperatures, the behaviour is still essentially unknown. Aims: Here we analyse combined SUMER (Solar Ultraviolet Measurements of Emitted Radiation)/SoHO (Solar and Heliospheric Observatory) and EIS (EUV Imaging Spectrometer)/Hinode observations of the quiet Sun around disk centre to determine, for the first time, the average Doppler shift of several spectral lines formed between 1 and 2 MK, where the largest part of the quiet coronal emission is formed. Methods: The measurements are based on a novel technique applied to EIS spectra to measure the difference in Doppler shift between lines formed at different temperatures. Simultaneous wavelength-calibrated SUMER spectra allow establishing the absolute value at the reference temperature of T ≈ 1 MK. Results: The average line shifts at 1 MK < T < 1.8 MK are modestly, but clearly bluer than those observed at 1 MK. By accepting an average blue shift of about (-1.8 ± 0.6) km s-1 at 1 MK (as provided by SUMER measurements), this translates into a maximum Doppler shift of (-4.4 ± 2.2) km s-1 around 1.8 MK. The measured value appears to decrease to about (-1.3 ± 2.6) km s-1 at the Fe xv formation temperature of 2.1 MK. Conclusions: The measured average Doppler shift between 0.01 and 2.1 MK, for which we provide a parametrisation, appears to be qualitatively and roughly quantitatively consistent with what foreseen by 3D coronal models where heating is produced by dissipation of currents induced by photospheric motions and by reconnection with emerging magnetic flux.

  6. Inclusive Jets in PHP

    NASA Astrophysics Data System (ADS)

    Roloff, P.

    Differential inclusive-jet cross sections have been measured in photoproduction for boson virtualities Q^2 < 1 GeV^2 with the ZEUS detector at HERA using an integrated luminosity of 300 pb^-1. Jets were identified in the laboratory frame using the k_T, anti-k_T or SIScone jet algorithms. Cross sections are presented as functions of the jet pseudorapidity, eta(jet), and the jet transverse energy, E_T(jet). Next-to-leading-order QCD calculations give a good description of the measurements, except for jets with low E_T(jet) and high eta(jet). The cross sections have the potential to improve the determination of the PDFs in future QCD fits. Values of alpha_s(M_Z) have been extracted from the measurements based on different jet algorithms. In addition, the energy-scale dependence of the strong coupling was determined.

  7. MK2206 in Treating Patients With Stage I, Stage II, or Stage III Breast Cancer

    ClinicalTrials.gov

    2015-03-16

    Estrogen Receptor Negative; Estrogen Receptor Positive; HER2/Neu Negative; HER2/Neu Positive; Progesterone Receptor Negative; Progesterone Receptor Positive; Stage IA Breast Cancer; Stage IB Breast Cancer; Stage IIA Breast Cancer; Stage IIB Breast Cancer; Stage IIIA Breast Cancer; Stage IIIB Breast Cancer; Stage IIIC Breast Cancer; Triple-Negative Breast Carcinoma

  8. New results on jet fragmentation at CDF

    SciTech Connect

    Jindariani, Sergo; /Florida U.

    2006-12-01

    Presented are the latest results of jet fragmentation studies at the Tevatron using the CDF Run II detector. Studies include the distribution of transverse momenta (Kt) of particles jets, two-particle momentum correlations, and indirectly global event shapes in p{bar p} collisions. Results are discussed within the context of recent Next-to-Leading Log calculations as well as earlier experimental results from the Tevatron and e{sup +}e{sup -} colliders.

  9. A deep Chandra observation of the poor cluster AWM 4 - II. The role of the radio jets in enriching the intracluster medium

    NASA Astrophysics Data System (ADS)

    O'Sullivan, Ewan; Giacintucci, Simona; David, Laurence P.; Vrtilek, Jan M.; Raychaudhury, Somak

    2011-03-01

    We use a Chandra observation of the poor cluster AWM 4 to map the temperature and abundance of the intracluster medium, so as to examine the influence of the central radio galaxy on its environment. While the cluster core is generally enriched to near-solar abundances, we find evidence of supersolar abundances correlated with the radio jets, extending ˜35 kpc from the core of the central dominant galaxy NGC 6051 along its minor-axis. We conclude that the enriched gas has been transported out of the central galaxy through the action of the radio source. We estimate the excess mass of iron in the entrained gas to be ˜1.4 × 106 M⊙ and find that this can be produced in the core of NGC 6051 within the time-scale of the active galactic nucleus (AGN) outburst. The energy required to transport this gas to its current location is ˜4.5 × 1057 erg, a significant fraction of the estimated total mechanical energy output of the AGN, though this estimate is dependent on the degree of enrichment of the uplifted gas. The larger near-solar abundance region is also compatible with enrichment by metals mixed outwards from NGC 6051 over a much longer time-scale.

  10. Antiviral Activity of MK-4965, a Novel Nonnucleoside Reverse Transcriptase Inhibitor▿

    PubMed Central

    Lai, Ming-Tain; Munshi, Vandna; Touch, Sinoeun; Tynebor, Robert M.; Tucker, Thomas J.; McKenna, Philip M.; Williams, Theresa M.; DiStefano, Daniel J.; Hazuda, Daria J.; Miller, Michael D.

    2009-01-01

    Nonnucleoside reverse transcriptase inhibitors (NNRTIs) are the mainstays of therapy for the treatment of human immunodeficiency virus type 1 (HIV-1) infections. However, the effectiveness of NNRTIs can be hampered by the development of resistance mutations which confer cross-resistance to drugs in the same class. Extensive efforts have been made to identify new NNRTIs that can suppress the replication of the prevalent NNRTI-resistant viruses. MK-4965 is a novel NNRTI that possesses both diaryl ether and indazole moieties. The compound displays potency at subnanomolar concentrations against wild-type (WT), K103N, and Y181C reverse transcriptase (RT) in biochemical assays. MK-4965 is also highly potent against the WT virus and two most prevalent NNRTI-resistant viruses (viruses that harbor the K103N or the Y181C mutation), against which it had 95% effective concentrations (EC95s) of <30 nM in the presence of 10% fetal bovine serum. The antiviral EC95 of MK-4965 was reduced approximately four- to sixfold when it was tested in 50% human serum. Moreover, MK-4965 was evaluated with a panel of 15 viruses with NNRTI resistance-associated mutations and showed a superior mutant profile to that of efavirenz but not to that of etravirine. MK-4965 was similarly effective against various HIV-1 subtypes and viruses containing nucleoside reverse transcriptase inhibitor or protease inhibitor resistance-conferring mutations. A two-drug combination study showed that the antiviral activity of MK-4965 was nonantagonistic with each of the 18 FDA-licensed drugs tested vice versa in the present study. Taken together, these in vitro data show that MK-4965 possesses the desired properties for further development as a new NNRTI for the treatment of HIV-1 infection. PMID:19289522

  11. Fast apoptosis and erythroid differentiation induced by imatinib mesylate in JURL-MK1 cells.

    PubMed

    Kuzelová, Katerina; Grebenová, Dana; Marinov, Iuri; Hrkal, Zbynek

    2005-05-15

    We compare the effects of Imatinib mesylate (Glivec) on chronic myeloid leukemia derived cell lines K562 and JURL-MK1. In both cell lines, the cell cycle arrests in G(1)/G(0) phase within 24 h after the addition of 1 microM Imatinib. This is followed by a decrease of Ki-67 expression and the induction of apoptosis. In JURL-MK1 cells, the apoptosis is faster in comparison with K562 cells: the caspase-3 activity reaches the peak value (20 to 30 fold of the control) after about 40 h and the apoptosis proceeds to its culmination point, the DNA fragmentation, within 48 h following 1 microM Imatinib addition. Unlike K562 cells, JURL-MK1 cells possess a probably functional p53 protein inducible by TPA (tetradecanoyl phorbol acetate) or UV-B irradiation. However, no increase in p53 expression was observed in Imatinib-treated JURL-MK1 cells indicating that the difference in the apoptosis rate between the two cell lines is not due to the lack of p53 in K562 cells. Imatinib also triggers erythroid differentiation both in JURL-MK1 and K562 cells. Glycophorin A expression occurred simultaneously with the apoptosis, even at the single cell level. In K562 cells, but not in JURL-MK1 cells, the differentiation process involved increased hemoglobin synthesis. However, during spontaneous evolution of JURL-MK1 cells in culture, the effects produced by Imatinib progressively changed from the fast apoptosis to the more complete erythroid differentiation. We suggest that the apoptosis and the erythroid differentiation are parallel effects of Imatinib and their relative contributions, kinetics and completeness are related to the differentiation stage of the treated cells. PMID:15770664

  12. Blockade of voltage-gated K⁺ currents in rat mesenteric arterial smooth muscle cells by MK801.

    PubMed

    Kim, Jeong Min; Park, Sang Woong; Lin, Hai Yue; Shin, Kyung Chul; Sung, Dong Jun; Kim, Jae Gon; Cho, Hana; Kim, Bokyung; Bae, Young Min

    2015-01-01

    MK801 (dizocilpine), a phencyclidine (PCP) derivative, is a potent noncompetitive antagonist of the N-Methyl-D-aspartate receptor (NMDAr). Another PCP derivative, ketamine, was reported to block voltage-gated K(+) (Kv) channels, which was independent of NMDAr function. Kv currents are major regulators of the membrane potential (Em) and excitability of muscles and neurons. Here, we investigated the effect of MK801 on the Kv channels and Em in rat mesenteric arterial smooth muscle cells (RMASMCs). We used the whole-cell patch clamp technique to analyze the effect of MK801 enantiomers on Kv channels and Em. (+)MK801 inhibited Kv channels in a concentration-dependent manner (IC50 of 89.1 ± 13.1 μM, Hill coefficient of 1.05 ± 0.08). The inhibition was voltage- and state- independent. (+)MK801 didn't influence steady-state activation and inactivation of Kv channels. (+)MK801 treatment depolarized Em in a concentration-dependent manner and concomitantly decreased membrane conductance. (-)MK801 also similarly inhibited the Kv channels (IC50 of 134.0 ± 17.5 μM, Hill coefficient of 0.87 ± 0.09). These results indicate that MK801 directly inhibits the Kv channel in a state-independent manner in RMASMCs. This MK801-mediated inhibition of Kv channels should be considered when assessing the various pharmacological effects produced by MK801, such as schizophrenia, neuroprotection, and hypertension. PMID:25704024

  13. Corporate Jet

    NASA Technical Reports Server (NTRS)

    1991-01-01

    Gulfstream Aerospace Corporation, Savannah, GA, used a version of a NASA program called WIBCO to design a wing for the Gulfstream IV (G-IV) which will help to reduce transonic drag (created by shock waves that develop as an airplane approaches the speed of sound). The G-IV cruises at 88 percent of the speed of sound, and holds the international record in its class for round-the-world flight. They also used the STANS5 and Profile programs in the design. They will use the NASA program GASP to help determine the gross weight, range, speed, payload and optimum wing area of an intercontinental supersonic business jet being developed in cooperation with Sukhoi Design Bureau, a Soviet organization.

  14. Jet inclusive cross sections

    SciTech Connect

    Del Duca, V.

    1992-11-01

    Minijet production in jet inclusive cross sections at hadron colliders, with large rapidity intervals between the tagged jets, is evaluated by using the BFKL pomeron. We describe the jet inclusive cross section for an arbitrary number of tagged jets, and show that it behaves like a system of coupled pomerons.

  15. Inverse agonist and pharmacochaperone properties of MK-0524 on the prostanoid DP1 receptor.

    PubMed

    Labrecque, Pascale; Roy, Sébastien J; Fréchette, Louis; Iorio-Morin, Christian; Gallant, Maxime A; Parent, Jean-Luc

    2013-01-01

    Prostaglandin D₂ (PGD₂) acts through two G protein-coupled receptors (GPCRs), the prostanoid DP receptor and CRTH2 also known as DP1 and DP2, respectively. Several previously characterized GPCR antagonists are now classified as inverse agonists and a number of GPCR ligands are known to display pharmacochaperone activity towards a given receptor. Here, we demonstrate that a DP1 specific antagonist, MK-0524 (also known as laropiprant), decreased basal levels of intracellular cAMP produced by DP1, a Gα(s)-coupled receptor, in HEK293 cells. This reduction in cAMP levels was not altered by pertussis toxin treatment, indicating that MK-0524 did not induce coupling of DP1 to Gα(i/o) proteins and that this ligand is a DP1 inverse agonist. Basal ERK1/2 activation by DP1 was not modulated by MK-0524. Interestingly, treatment of HEK293 cells expressing Flag-tagged DP1 with MK-0524 promoted DP1 cell surface expression time-dependently to reach a maximum increase of 50% compared to control after 24 h. In contrast, PGD₂ induced the internalization of 75% of cell surface DP1 after the same time of stimulation. The increase in DP1 cell surface targeting by MK-0524 was inhibited by Brefeldin A, an inhibitor of transport from the endoplasmic reticulum-Golgi to the plasma membrane. Confocal microscopy confirmed that a large population of DP1 remained trapped intracellularly and co-localized with calnexin, an endoplasmic reticulum marker. Redistribution of DP1 from intracellular compartments to the plasma membrane was observed following treatment with MK-0524 for 24 h. Furthermore, MK-0524 promoted the interaction between DP1 and the ANKRD13C protein, which we showed previously to display chaperone-like effects towards the receptor. We thus report that MK-0524 is an inverse agonist and a pharmacochaperone of DP1. Our findings may have important implications during therapeutic treatments with MK-0524 and for the development of new molecules targeting DP1. PMID:23762421

  16. Supersonic gas jets

    NASA Astrophysics Data System (ADS)

    Dulov, V. G.

    The papers presented in this volume provide an overview of the current state of research in the gas dynamics of jet flows. In particular, attention is given to free supersonic jets and to the interaction of supersonic jets with one another and with obstacles under stationary and nonstationary flow conditions. Papers are presented on a method for calculating a weakly anisotropic supersonic turbulent jet in a subsonic slipstream; composite supersonic jets; the principal gas-dynamic characteristics of the processes occurring in gas-jet-driven shock-wave generators; and the construction of models for supersonic jet flows. For individual items see A84-16902 to A84-16918

  17. "Waveguidability" of idealized jets

    NASA Astrophysics Data System (ADS)

    Manola, Iris; Selten, Frank; Vries, Hylke; Hazeleger, Wilco

    2013-09-01

    It is known that strong zonal jets can act as waveguides for Rossby waves. In this study we use the European Center for Medium-Range Weather Forecasts (ECMWF) reanalysis data to analyze the connection between jets and zonal waves at timescales beyond 10 days. Moreover, a barotropic model is used to systematically study the ability of idealized jets to trap Rossby wave energy ("waveguidability") as a function of jet strength, jet width, and jet location. In general, strongest waveguidability is found for narrow, fast jets. In addition, when the stationary wave number is integer, a resonant response is found through constructive interference. In Austral summer, the Southern Hemispheric jet is closest to the idealized jets considered and it is for this season that similar jet-zonal wave relationships are identified in the ECMWF reanalysis data.

  18. GM1 ganglioside reverses the cognitive deficits induced by MK801 in mice.

    PubMed

    Ni, Yu-Fei; Zhang, Wei; Bao, Xiao-Feng; Wang, Wei; Song, Lu; Jiang, Bo

    2016-08-01

    Cognitive deficits are core symptoms of schizophrenia, but effective treatments are still lacking. Previous studies have reported that the brain-derived neurotrophic factor (BDNF) signaling is closely involved in learning and memory. Monosialotetrahexosylganglioside (GM1) is a ganglioside with wide-ranging pharmacologic effects that enhances the BDNF signaling cascade. This study aimed to assess the effects of GM1 on schizophrenia-related cognitive impairments. A brief disruption of N-methyl-D-aspartate receptors with MK801 was used to generate the animal model for cognitive deficits in schizophrenia. It was found that MK801-treated mice showed significant deficits in memory ability compared with control mice in different behavior tests, and this was accompanied by decreased hippocampal BDNF signaling pathway. Consecutive administration of GM1 fully restored the MK801-induced cognitive deficits and the impaired BDNF signaling in the hippocampus. Furthermore, a BDNF system inhibitor abolished the effects of GM1 in the MK801 model. Taken together, our results show that GM1 could reverse the MK801-induced cognitive deficits, suggesting a potential usefulness of GM1 in treating the schizophrenia-related cognitive impairments. PMID:26960162

  19. Effect of MK-801 and Clozapine on the Proteome of Cultured Human Oligodendrocytes

    PubMed Central

    Cassoli, Juliana S.; Iwata, Keiko; Steiner, Johann; Guest, Paul C.; Turck, Christoph W.; Nascimento, Juliana M.; Martins-de-Souza, Daniel

    2016-01-01

    Separate lines of evidence have demonstrated the involvement of N-methyl-D-aspartate (NMDA) receptor and oligodendrocyte dysfunctions in schizophrenia. Here, we have carried out shotgun mass spectrometry proteome analysis of oligodendrocytes treated with the NMDA receptor antagonist MK-801 to gain potential insights into these effects at the molecular level. The MK-801 treatment led to alterations in the levels of 68 proteins, which are associated with seven distinct biological processes. Most of these proteins are involved in energy metabolism and many have been found to be dysregulated in previous proteomic studies of post-mortem brain tissues from schizophrenia patients. Finally, addition of the antipsychotic clozapine to MK-801-treated oligodendrocyte cultures resulted in changes in the levels of 45 proteins and treatment with clozapine alone altered 122 proteins and many of these showed opposite changes to the MK-801 effects. Therefore, these proteins and the associated energy metabolism pathways should be explored as potential biomarkers of antipsychotic efficacy. In conclusion, MK-801 treatment of oligodendrocytes may provide a useful model for testing the efficacy of novel treatment approaches. PMID:26973466

  20. Properties of Mlso MK3 White-Light CMEs from 1989-1996

    NASA Astrophysics Data System (ADS)

    St Cyr, O. C.; Flint, Q.; Xie, H.; Webb, D. F.; Burkepile, J.; Lecinski, A. R.

    2014-12-01

    Observations low in the solar corona are key to understanding the initiation and propagation of coronal mass ejections (CMEs). Similar to solar magnetographs and total solar irradiance measurements, white-light coronagraph observations are another aspect of Heliophysics that have followed a path comparable to that found in Earth science, where longevity and continuity of measurements lead to new understanding through modeling. Previously we have reported on our efforts to fill the only remaining data gap since 1973 in the CME rate using the Mauna Loa Solar Observatory's MK3 K-coronameter observations from 1989-1996. The MK3 instrument observed routinely several hours most days beginning in 1980 until it was upgraded to MK4 in 1998. MK3 CMEs detected from 1980-1989 were compared with Solwind and SMM and reported by St. Cyr et al. (1999). Here we provide a preliminary report on the statistical properties of CMEs detected during 1989-1996. Since this period began in solar maximum activity conditions and descended into minimum, we can compare the properties of MLSO MK3 events with CMEs detected by other coronagraphs during similar activity phases.

  1. Decreased hyperthermic effect of MK-801 in selectively bred hypercholinergic rats.

    PubMed

    Pucilowski, O; Danysz, W; Overstreet, D H; Rezvani, A H; Eichelman, B; Janowsky, D S

    1991-04-01

    The Flinders Sensitive Line (FSL) of rats has been selectively bred to have increased sensitivity to cholinergic agonists. However, these rats exhibit altered responsiveness to a number of noncholinergic agents, such as apomorphine, buspirone and ethanol. This study compared the FSL and control Flinders Resistant Line (FRL) rats in terms of their hyperthermic response to the phencyclidine (PCP) receptor agonist, MK-801 (0.2 mg/kg SC) and their MK-801 binding characteristics. We have found that FSL rats react with a delayed hyperthermia, having a significantly lower hyperthermia for the first 120 min of observation. Thereafter the response does not differ in FSL and FRL rats. Both groups had similar affinities and numbers of [3H]MK-801 binding sites in the hippocampus/cerebral cortex. Pretreatment with scopolamine (1 mg/kg SC) failed to affect MK-801-induced hyperthermia in either line of rats. These findings suggest that selective breeding of FSL rats attenuated the secondary mechanisms involved in the PCP receptor-mediated hyperthermic response. However, by itself cholinergic supersensitivity does not appear to be a major factor in the blunted responsiveness of FSL rats to MK-801. PMID:1868361

  2. Agmatine enhances antidepressant potency of MK-801 and conventional antidepressants in mice.

    PubMed

    Neis, Vivian Binder; Moretti, Morgana; Manosso, Luana Meller; Lopes, Mark W; Leal, Rodrigo Bainy; Rodrigues, Ana Lúcia S

    2015-03-01

    Agmatine, an endogenous guanidine amine, has been shown to produce antidepressant-like effects in animal studies. This study investigated the effects of the combined administration of agmatine with either conventional monoaminergic antidepressants or the N-methyl-D-aspartate (NMDA) receptor antagonist MK-801 in the tail suspension test (TST) in mice. The aim was to evaluate the extent of the antidepressant synergism by examining the ability of a fixed dose of agmatine to shift the antidepressant potency of fluoxetine, imipramine, bupropion and MK-801. A sub-effective dose of agmatine (0.0001 mg/kg, p.o.) significantly increased the potency by which fluoxetine, imipramine, bupropion and MK-801 decreased immobility time in the TST by 2-fold (fluoxetine), 10-fold (imipramine and bupropion) and 100-fold (MK-801). Combined with previous evidence indicating a role of monoaminergic systems in the effect of agmatine, the current data suggest that agmatine may modulate monoaminergic neurotransmission and augment the activity of conventional antidepressants. Moreover, this study found that agmatine substantially augmented the antidepressant-like effect of MK-801, reinforcing the notion that this compound modulates NMDA receptor activation. These preclinical data may stimulate future clinical studies testing the effects of augmentation therapy with agmatine for the management of depressive disorders. PMID:25553821

  3. A natural therapeutic approach for the treatment of periodontitis by MK615.

    PubMed

    Morimoto-Yamashita, Yoko; Kawakami, Yoshiko; Tatsuyama, Syoko; Miyashita, Keiko; Emoto, Makiko; Kikuchi, Kiyoshi; Kawahara, Ko-ichi; Tokuda, Masayuki

    2015-11-01

    Periodontitis is a chronic inflammatory disease that affects the tooth-supporting tissues. Gingival fibroblasts are the most abundant cells in periodontal tissues and they participate actively in the host inflammatory response to periodontal pathogens that is known to mediate local tissue destruction in periodontitis. The Japanese apricot, known as Ume in Japanese, has been a traditional Japanese medicine for centuries and is a familiar and commonly consumed food. The health benefits of Ume are widely recognized and have been confirmed in recent studies showing that MK615, an extract of compounds from Ume, has strong anticancer and anti-inflammatory effects. However, the potential role of MK615 in oral health is unknown. We hypothesized that the anti-inflammatory activities of MK615 could be exploited to inhibit the effects of lipopolysaccharide (LPS) produced by periodontal bacterial pathogens, such as Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Here, we show that LPS-induced interleukin (IL)-6 and IL-8 production by gingival fibroblasts was dose-dependently inhibited by MK615. As a potent inhibitor of the inflammatory responses induced by periodontal pathogens, MK615 merits further testing as a therapeutic agent in inflammatory diseases such as periodontitis. PMID:26305447

  4. MK615: a new therapeutic approach for the treatment of oral disease.

    PubMed

    Morimoto-Yamashita, Yoko; Matsuo, Miki; Komatsuzawa, Hitoshi; Kawahara, Ko-ichi; Kikuchi, Kiyoshi; Torii, Mitsuo; Tokuda, Masayuki

    2011-08-01

    The oral cavity is inhabited by over 500 different bacterial species. Dental caries and periodontitis are major bacterial infectious diseases in the oral cavity. Prunus mume Sieb. et Zucc., which is a variety of Japanese apricot known as Ume in Japanese, has been a traditional Japanese medicine for centuries, and is a familiar and commonly consumed food. The health benefits of Ume are now being widely recognized. Recent studies showed that MK615, an extract of compounds from Ume, has strong anticancer and anti-inflammatory effects. However, the potential role of MK615 in the antimicrobial field remains unknown. Therefore, we hypothesize that MK615 has antimicrobial activities against a range of oral bacterial pathogens. Here, we show that MK615 may be a potent inhibitor of the growth of some oral bacteria and an inhibitor of biofilm formation by Streptococcus mutans, the principal etiological agent of human dental caries. Our findings suggest that MK615 has potential as a therapeutic agent for treating and preventing oral diseases such as dental caries and periodontitis. PMID:21565449

  5. Age-related differential sensitivity to MK-801-induced locomotion and stereotypy in C57BL/6 mice

    PubMed Central

    Qi, Chunting; Zou, Hong; Zhang, Ruizhong; Zhao, Guoping; Jin, Meilei; Yu, Lei

    2009-01-01

    Psychomotor effects elicited by systemic administration of the noncompetitive NMDA (N-methyl-D-aspartate) receptor antagonist MK-801 (dizocilpine maleate) represent perturbation of glutamatergic pathways, providing an animal model for psychotic symptoms of schizophrenia. Hyperlocomotion and stereotypy are the two main psychomotor behaviors induced by MK-801. This study compared MK-801-induced hyperlocomotion and stereotypy in young (1-month old) and aged mice (12-month old), in order to determine how the aging process may influence these behaviors. The tested MK-801 doses ranged from 0.015 to 1 mg/kg. The data indicated that MK-801 impacted the aged mice more pronouncedly than the young mice, as both hyperlocomotion and stereotypy were increased significantly more in the aged mice relative to the young mice. These results suggest an age-related increase in MK-801 sensitivity in mice. PMID:18053981

  6. Sensorimotor gating impairments induced by MK-801 treatment may be reduced by tolerance effect and by familiarization in monkeys

    PubMed Central

    Saletti, Patricia G.; Maior, Rafael S.; Hori, Etsuro; Nishijo, Hisao; Tomaz, Carlos

    2015-01-01

    Dizocilpine (MK-801) is a non-competitive NMDA antagonist that induces schizophreniclike effects. It is therefore widely used in experimental models of schizophrenia including prepulse inhibition (PPI) impairments in rodents. Nevertheless, MK-801 has never been tested in monkeys on a PPI paradigm. In order to evaluate MK-801 effects on monkeys’ PPI, we tested eight capuchin monkeys (Sapajus spp.) using three different doses of MK-801 (0.01; 0.02; 0.03 mg/kg). Results show PPI impairment in acute administration of the highest dose (0.03 mg/kg). PPI impairment induced by MK-801 was reversed by re-exposure to the PPI test throughout treatment trials, in contrast with rodent studies. These results indicate that tolerance effect and familiarization with PPI test may reduce the sensorimotor gating deficits induced by MK-801 in monkeys, suggesting a drug-training interaction. PMID:26441660

  7. Inhibitory effects of KN-93, an inhibitor of Ca2+ calmodulin-dependent protein kinase II, on light-regulated root gravitropism in maize

    NASA Technical Reports Server (NTRS)

    Feldman, L. J.; Hidaka, H.

    1993-01-01

    Light is essential for root gravitropism in Zea mays L., cultivar Merit. It is hypothesized that calcium mediates this light-regulated response. KN-93, an inhibitor of calcium/calmodulin kinase II (CaMK II), inhibits light-regulated root gravitropism but does not affect light perception. We hypothesize that CaMK II, or a homologue, operates late in the light/gravity signal transduction chain. Here we provide evidence suggesting a possible physiological involvement of CaMK II in root gravitropism in plants.

  8. Akt inhibitor MK2206 prevents influenza pH1N1 virus infection in vitro.

    PubMed

    Denisova, Oxana V; Söderholm, Sandra; Virtanen, Salla; Von Schantz, Carina; Bychkov, Dmitrii; Vashchinkina, Elena; Desloovere, Jens; Tynell, Janne; Ikonen, Niina; Theisen, Linda L; Nyman, Tuula A; Matikainen, Sampsa; Kallioniemi, Olli; Julkunen, Ilkka; Muller, Claude P; Saelens, Xavier; Verkhusha, Vladislav V; Kainov, Denis E

    2014-07-01

    The influenza pH1N1 virus caused a global flu pandemic in 2009 and continues manifestation as a seasonal virus. Better understanding of the virus-host cell interaction could result in development of better prevention and treatment options. Here we show that the Akt inhibitor MK2206 blocks influenza pH1N1 virus infection in vitro. In particular, at noncytotoxic concentrations, MK2206 alters Akt signaling and inhibits endocytic uptake of the virus. Interestingly, MK2206 is unable to inhibit H3N2, H7N9, and H5N1 viruses, indicating that pH1N1 evolved specific requirements for efficient infection. Thus, Akt signaling could be exploited further for development of better therapeutics against pH1N1 virus. PMID:24752266

  9. Discovery of selective and orally available spiro-3-piperidyl ATP-competitive MK2 inhibitors.

    PubMed

    Kaptein, Allard; Oubrie, Arthur; de Zwart, Edwin; Hoogenboom, Niels; de Wit, Joeri; van de Kar, Bas; van Hoek, Maaike; Vogel, Gerard; de Kimpe, Vera; Schultz-Fademrecht, Carsten; Borsboom, Judith; van Zeeland, Mario; Versteegh, Judith; Kazemier, Bert; de Roos, Jeroen; Wijnands, Frank; Dulos, John; Jaeger, Martin; Leandro-Garcia, Paula; Barf, Tjeerd

    2011-06-15

    The identification of a potent, selective, and orally available MK2 inhibitor series is described. The initial absence of oral bioavailability was successfully tackled by moving the basic nitrogen of the spiro-4-piperidyl moiety towards the electron-deficient pyrrolepyridinedione core, thereby reducing the pK(a) and improving Caco-2 permeability. The resulting racemic spiro-3-piperidyl analogues were separated by chiral preparative HPLC, and the activity towards MK2 inhibition was shown to reside mostly in the first eluting stereoisomer. This led to the identification of new MK2 inhibitors, such as (S)-23, with low nanomolar biochemical inhibition (EC(50) 7.4 nM) and submicromolar cellular target engagement activity (EC(50) 0.5 μM). PMID:21565498

  10. Akt Inhibitor MK2206 Prevents Influenza pH1N1 Virus Infection In Vitro

    PubMed Central

    Denisova, Oxana V.; Söderholm, Sandra; Virtanen, Salla; Von Schantz, Carina; Bychkov, Dmitrii; Vashchinkina, Elena; Desloovere, Jens; Tynell, Janne; Ikonen, Niina; Theisen, Linda L.; Nyman, Tuula A.; Matikainen, Sampsa; Kallioniemi, Olli; Julkunen, Ilkka; Muller, Claude P.; Saelens, Xavier; Verkhusha, Vladislav V.

    2014-01-01

    The influenza pH1N1 virus caused a global flu pandemic in 2009 and continues manifestation as a seasonal virus. Better understanding of the virus-host cell interaction could result in development of better prevention and treatment options. Here we show that the Akt inhibitor MK2206 blocks influenza pH1N1 virus infection in vitro. In particular, at noncytotoxic concentrations, MK2206 alters Akt signaling and inhibits endocytic uptake of the virus. Interestingly, MK2206 is unable to inhibit H3N2, H7N9, and H5N1 viruses, indicating that pH1N1 evolved specific requirements for efficient infection. Thus, Akt signaling could be exploited further for development of better therapeutics against pH1N1 virus. PMID:24752266

  11. MK5 activates Rag transcription via Foxo1 in developing B cells

    PubMed Central

    Chow, Kwan T.; Timblin, Greg A.; McWhirter, Sarah M.

    2013-01-01

    Foxo1 is a critical, direct regulator of Rag (recombination activating gene) transcription during B cell development and is thus essential for the generation of a diverse repertoire of antigen receptors. Although Foxo1 regulation has been widely studied in many cell types, pathways regulating Foxo1 in B cells have not been fully elucidated. By screening a panel of Foxo1 mutants, we identified serine 215 on Foxo1 as a novel phosphorylation site that is essential for the activation of Rag transcription. Mutation of S215 strongly attenuated transactivation of Rag but did not affect most other Foxo1 target genes. We show that MK5, a MAPK-activated protein kinase, is a previously unidentified upstream regulator of Foxo1. MK5 was necessary and sufficient to activate Rag transcription in transformed and primary pro–B cells. Together, our experiments show that MK5 positively regulates Rag transcription via phosphorylation of Foxo1 in developing B cells. PMID:23878308

  12. Evolution of the Jet Opening Angle Distribution in Holographic Plasma

    NASA Astrophysics Data System (ADS)

    Rajagopal, Krishna; Sadofyev, Andrey V.; van der Schee, Wilke

    2016-05-01

    We use holography to analyze the evolution of an ensemble of jets, with an initial probability distribution for their energy and opening angle as in proton-proton (p p ) collisions, as they propagate through an expanding cooling droplet of strongly coupled plasma as in heavy ion collisions. We identify two competing effects: (i) each individual jet widens as it propagates and (ii) because wide-angle jets lose more energy, energy loss combined with the steeply falling perturbative spectrum serves to filter wide jets out of the ensemble at any given energy. Even though every jet widens, jets with a given energy can have a smaller mean opening angle after passage through the plasma than jets with that energy would have had in vacuum, as experimental data may indicate.

  13. Evolution of the Jet Opening Angle Distribution in Holographic Plasma.

    PubMed

    Rajagopal, Krishna; Sadofyev, Andrey V; van der Schee, Wilke

    2016-05-27

    We use holography to analyze the evolution of an ensemble of jets, with an initial probability distribution for their energy and opening angle as in proton-proton (pp) collisions, as they propagate through an expanding cooling droplet of strongly coupled plasma as in heavy ion collisions. We identify two competing effects: (i) each individual jet widens as it propagates and (ii) because wide-angle jets lose more energy, energy loss combined with the steeply falling perturbative spectrum serves to filter wide jets out of the ensemble at any given energy. Even though every jet widens, jets with a given energy can have a smaller mean opening angle after passage through the plasma than jets with that energy would have had in vacuum, as experimental data may indicate. PMID:27284647

  14. MK-4827, a PARP-1/-2 inhibitor, strongly enhances response of human lung and breast cancer xenografts to radiation.

    PubMed

    Wang, Li; Mason, Kathy A; Ang, K Kian; Buchholz, Thomas; Valdecanas, David; Mathur, Anjili; Buser-Doepner, Carolyn; Toniatti, Carlo; Milas, Luka

    2012-12-01

    The poly-(ADP-ribose) polymerase (PARP) inhibitor, MK-4827, is a novel potent, orally bioavailable PARP-1 and PARP-2 inhibitor currently in phase I clinical trials for cancer treatment. No preclinical data currently exist on the combination of MK-4827 with radiotherapy. The current study examined combined treatment efficacy of MK-4827 and fractionated radiotherapy using a variety of human tumor xenografts of differing p53 status: Calu-6 (p53 null), A549 (p53 wild-type [wt]) and H-460 (p53 wt) lung cancers and triple negative MDA-MB-231 human breast carcinoma. To mimic clinical application of radiotherapy, fractionated radiation (2 Gy per fraction) schedules given once or twice daily for 1 to 2 weeks combined with MK-4827, 50 mg/kg once daily or 25 mg/kg twice daily, were used. MK-4827 was found to be highly and similarly effective in both radiation schedules but maximum radiation enhancement was observed when MK-4827 was given at a dose of 50 mg/kg once daily (EF = 2.2). MK-4827 radiosensitized all four tumors studied regardless of their p53 status. MK-4827 reduced PAR levels in tumors by 1 h after administration which persisted for up to 24 h. This long period of PARP inhibition potentially adds to the flexibility of design of future clinical trials. Thus, MK-4827 shows high potential to improve the efficacy of radiotherapy. PMID:22127459

  15. Therapeutic effect of the NMDA antagonist MK-801 on low-level laser induced retinal injury

    NASA Astrophysics Data System (ADS)

    Yan, W.-H.; Wu, J.; Chen, P.; Dou, J.-T.; Pan, C.-Y.; Mu, Y.-M.; Lu, J.-M.

    2009-03-01

    The aim of this article was to explore the mechanism of injury in rat retina after constant low-level helium-neon (He-Ne) laser exposure and therapeutic effects of MK-801, an N-methyl-D-aspartate (NMDA) receptor antagonist, on laser-induced retinal injury. He-Ne laser lesions were created in the central retina of adult Wistar Kyoto rats and were followed immediately by intraperitoneal injection of MK-801 (2 mg/kg) or saline, macroscopical and microscopical lesion were observed by funduscope and light microscope. Ultrastructural changes of the degenerating cells were examined by electron microscopy. Photoreceptor apoptosis was evaluated by TdT-mediated dUTP nick end-labeling (TUNEL). mRNA levels were measured by in situ hybridization and NMDA receptor expression was determined by immunohistochemistry. Laser induced damage was histologically quantified by image-analysis morphometry. Electroretinograms (ERGs) were recorded at different time point after the cessation of exposure to constant irradiation. There was no visible bleeding, exudation or necrosis under funduscope. TUNEL and electron microscopy showed photoreceptor apoptosis after irradiation. MK-801-treated animals had significantly fewer TUNEL-positive cells in the photoreceptors than saline-treated animals after exposure to laser. In situ hybridization (ISH) showed that the NMDAR mRNA level of MK-801-treated rats decreased in the inner plexiform layer 6 h after the cessation of exposure to constant irradiation when compared with that of saline-treated rats. So did Immunohistochemistry (IHC). Electroretinogram showed that b-wave amplitudes of MK-801-treated group were higher than that of saline-treated group after laser exposure. These findings suggest that Low level laser may cause the retinal pathological changes under given conditions. High expression of NMDAR is one of the possible mechanisms causing experimental retinal laser injury of rats. MK-801 exhibits the therapeutic effect due to promote the

  16. Effects of Dipeptidyl Peptidase-4 Inhibition with MK-0431 on Syngeneic Mouse Islet Transplantation

    PubMed Central

    Juang, Jyuhn-Huarng; Kuo, Chien-Hung; Liu, Ying-Hsiu; Chang, Han-Ying; Chen, Chiung-Tong

    2014-01-01

    Dipeptidyl peptidase (DPP)-4 inhibitors increase circulating levels of glucagon-like peptide-1 and glucose-dependent insulinotropic polypeptide which may promote β-cell proliferation and survival. This study tested if DPP-4 inhibition with MK-0431 is beneficial for diabetic mice syngeneically transplanted with a marginal number of islets. We syngeneically transplanted 150 C57BL/6 mouse islets under the kidney capsule of each streptozotocin-diabetic mouse and then treated recipients with (n = 21) or without (n = 17) MK-0431 (30 mg/kg/day, po) for 6 weeks. After islet transplantation, blood glucose levels decreased in both MK-0431-treated and control groups. However, the blood glucose and area under the curve of the intraperitoneal glucose tolerance test at 2, 4, and 6 weeks were not significantly different between MK-0431-treated mice and controls. During 6 weeks, both groups exhibited increased body weights over time. However, the weight between two groups did not differ throughout the study period. At 6 weeks after transplantation, the graft beta-cell mass (0.024 ± 0.005 versus 0.023 ± 0.007 mg, P = 0.8793) and insulin content (140 ± 48 versus 231 ± 63 ng, P = 0.2939) were comparable in the MK-0431-treated group and controls. Our results indicate posttransplant DPP-4 inhibition with MK-0431 in the diabetic recipient with a marginal number of islets is not beneficial to transplantation outcome or islet grafts. PMID:25165473

  17. Attenuation of MK-801-induced behavioral perseveration by typical and atypical antipsychotic pretreatment in rats.

    PubMed

    Tuplin, Erin W; Stocco, Marlaina R; Holahan, Matthew R

    2015-08-01

    The noncompetitive NMDA receptor antagonist (+)-5-methyl-10,11-dihydro-5H-dibenzo [a,d] cyclohepten-5-10-imine maleate (MK-801) has been shown to increase the probability of operant responding during extinction and reduce infralimbic prefrontal cortical activation, possibly modeling the cognitive dysfunction symptomology, and underlying cause, in patients with schizophrenia. The present study sought to determine if typical and/or atypical antipsychotics would attenuate the MK-801-induced behavioral perseveration and whether this would be associated with concomitant changes in phosphorylated ERK1/2 (pERK1/2) labeling in the infralimbic cortex (IL). Male, Long Evans rats were pretreated with the typical antipsychotic, Flupenthixol (0, 0.125, 0.25 or 0.5 mg/kg) or the atypical antipsychotic, aripiprazole (0, 0.3, 1.0, 3.0 mg/kg), then given 0.1 mg/kg MK-801 followed by a 60-min appetitive operant extinction session. Flupenthixol produced a dose-dependent decrease in MK-801-induced bar pressing behavior and locomotor activity and a dose-dependent increase in IL pERK1/2 labeling. Aripiprazole produced a U-shaped dose-response curve on MK-801-induced bar pressing behavior, a dose-dependent decrease in locomotor activity but no changes in IL pERK1/2 labeling. The attenuation of the MK-801-induced behavioral (bar pressing, locomotion) profile by Flupenthixol indicates a clear dopaminergic contribution to this behavior. The behavioral effect of aripiprazole may be due to its a) binding to presynaptic dopamine receptors at the midrange dose decreasing dopamine output and b) binding to postsynaptic dopamine receptors at the higher dose increasing dopamine tone. While both classes of antipsychotics can normalize perseverative behavioral symptoms, the underlying prefrontal cortical dysregulation seems to persist. PMID:26052791

  18. Supplemental Reactor Physics Calculations and Analysis of ELF Mk 1A Fuel

    SciTech Connect

    Michael A. Pope

    2014-10-01

    These calculations supplement previous the reactor physics work evaluating the Enhanced Low Enriched Uranium (LEU) Fuel (ELF) Mk 1A element. This includes various additional comparisons between the current Highly Enriched Uranium (HEU) and LEU along with further characterization of the performance of the ELF fuel. The excess reactivity to be held down at BOC for ELF Mk 1A fuel is estimated to be approximately $2.75 greater than with HEU for a typical cycle. This is a combined effect of the absence of burnable poison in the ELF fuel and the reduced neck shim worth in LEU fuel compared to HEU. Burnable poison rods were conceptualized for use in the small B positions containing Gd2O3 absorber. These were shown to provide $2.37 of negative reactivity at BOC and to burn out in less than half of a cycle. The worth of OSCCs is approximately the same between HEU and ELF Mk 1A (LEU) fuels in the representative loading evaluated. This was evaluated by rotating all banks simultaneously. The safety rod worth is relatively unchanged between HEU and ELF Mk 1A (LEU) fuels in the representative loading evaluated. However, this should be reevaluated with different loadings. Neutron flux, both total and fast (>1 MeV), is either the same or reduced upon changing from HEU to ELF Mk 1A (LEU) fuels in the representative loading evaluated. This is consistent with the well-established trend of lower neutron fluxes for a given power in LEU than HEU.The IPT loop void reactivity is approximately the same or less positive with ELF Mk 1A (LEU) fuel than HEU in the representative loading evaluated.

  19. The HH 24 Jet Complex: Collimated and Colliding Jets from a Newborn Multiple Stellar System

    NASA Astrophysics Data System (ADS)

    Reipurth, Bo

    2013-10-01

    The HH 24 complex constitutes the richest concentration of collimated bright Herbig-Haro jets known, and they originate from a small grouping of newborn binary and multiple systems. At least 6 jets are identified in deep groundbased optical interference images, and a similar number of sources in infrared images. We propose to do the first HST study of this complex, using H-alpha and [SII] filters. HST 0.05" to 0.1" angular resolution {20 to 40 AU at d 400 pc} is needed to resolve the shocks and their post-shock cooling layers for comparison with advanced numerical modeling. Our emphasis here is to explore outflows from a multiple system of newborn stars. Many of the jets show clear evidence of wiggling. The theory of jet motion from binary systems coupled with disk precession is now understood, and we will interpret the jet wiggles in this framework. Additionally, two of the HH 24 jets are showing evidence for a collision, a unique situation not seen anywhere else, and HST resolution is needed for comparison with gas-dynamic studies of jet-jet collisions. Two of the HH 24 jets are bright in the infrared [FeII] 1.644 line. In this line the main jet can be traced all the way to the source, which is the most important region for understanding the effects of binarity on the jet structure. We also apply for a second-epoch [SII] image in Cycle 23. This allows us, in addition to deriving the bulk motion, to determine such processes as expansion of the jet beam, sideways ejection in a working surface, turbulent and chaotic motions, and the effect of instabilities.

  20. A continuous dry 300 mK cooler for THz sensing applications

    NASA Astrophysics Data System (ADS)

    Klemencic, G. M.; Ade, P. A. R.; Chase, S.; Sudiwala, R.; Woodcraft, A. L.

    2016-04-01

    We describe and demonstrate the automated operation of a novel cryostat design that is capable of maintaining an unloaded base temperature of less than 300 mK continuously, without the need to recycle the gases within the final cold head, as is the case for conventional single shot sorption pumped 3He cooling systems. This closed dry system uses only 5 l of 3He gas, making this an economical alternative to traditional systems where a long hold time is required. During testing, a temperature of 365 mK was maintained with a constant 20 μW load, simulating the cooling requirement of a far infrared camera.

  1. Inhibition of Morphine Tolerance and Dependence by the NMDA Receptor Antagonist MK-801

    NASA Astrophysics Data System (ADS)

    Trujillo, Keith A.; Akil, Huda

    1991-01-01

    The N-methyl-D-aspartate (NMDA) subtype of the glutamate receptor is an important mediator of several forms of neural and behavioral plasticity. The present studies examined whether NMDA receptors might be involved in the development of opiate tolerance and dependence, two examples of behavioral plasticity. The noncompetitive NMDA receptor antagonist MK-801 attenuated the development of tolerance to the analgesic effect of morphine without affecting acute morphine analgesia. In addition, MK-801 attenuated the development of morphine dependence as assessed by naloxone-precipitated withdrawal. These results suggest that NMDA receptors may be important in the development of opiate tolerance and dependence.

  2. The impact of medetomidine on the protein-binding characteristics of MK-467 in canine plasma.

    PubMed

    Bennett, R C; Hokkanen, J; Raekallio, M R; Vainio, O M

    2016-08-01

    This study determined the unbound fraction of the peripheral α2 -adrenoceptor antagonist MK-467 alone and combined with medetomidine. MK-467 (0.1, 1 and 10 μm) was incubated in canine plasma with and without medetomidine (molar ratio 20:1), with human serum albumin (HSA) and with α1-acid glycoprotein (AGP). Rapid equilibrium dialysis was used for the measurement of protein binding. All samples were analysed by liquid chromatography and tandem mass spectrometry to obtain the unbound fraction (fu ) of MK-467. Unbound fractions (fu ) of MK-467 in canine plasma (mean ± standard deviation) were 27.6 ± 3.5%, 26.6 ± 0.9% and 42.4 ± 1.2% at 0.1, 1.0 and 10 μm concentrations, respectively. In the presence of medetomidine, fu were 27.5 ± 0.4%, 26.6 ± 0.9% and 41.0 ± 2.4%. The fu of MK-467 in HSA were 50.1 ± 2.5% at 0.1 μm, 49.4 ± 1.2% at 1.0 μm and 56.7 ± 0.5% at 10 μm. fu of MK-467 in AGP was 56.3 ± 3.7% at 0.1 μm, 54.6 ± 5.6% at 1.0 μm and 65.3 ± 0.4% at 10 μm. Protein binding of MK-467 was approximately 70% between 0.1 and 1.0 μm. Medetomidine had no apparent effect on the protein binding of MK-467. PMID:26763140

  3. Preliminary animal pharmacokinetics of the parenteral antifungal agent MK-0991 (L-743,872).

    PubMed

    Hajdu, R; Thompson, R; Sundelof, J G; Pelak, B A; Bouffard, F A; Dropinski, J F; Kropp, H

    1997-11-01

    MK-0991 (L-743,872) is a potent antifungal agent featuring long half-life pharmacokinetics. The pharmacokinetics of MK-0991 administered intravenously to mice, rats, rhesus monkeys, and chimpanzees is presented. Unique to MK-0991 is its consistent cross-species performance. The range of values for the pharmacokinetic parameters were as follows: clearance, 0.26 to 0.51 ml/min/kg; half-life, 5.2 to 7.6 h; and distributive volume, 0.11 to 0.27 liters/kg. The level of protein binding of MK-0991 was determined to be 96% in mouse and human serum. The compound exhibited high affinities for human serum albumin and at least two lipid components. The rationale for the selection of MK-0991 as a drug development candidate was based on its two- to threefold superior pharmacokinetic performance in chimpanzees over the performance of an otherwise equivalent analog, L-733,560. Once-daily dosing for MK-0991 is indicated by a graphical comparison of levels in the circulations of chimpanzees and mice. In a study of the pharmacokinetics of MK-0991 in mouse tissue, the organs were assayed following intraperitoneal administration. The area under the concentration-versus-time curves (AUC) segregated the tissues into three exposure categories relative to plasma. The tissues with greater exposure than that for plasma were liver (16 times), kidney (3 times), and large intestine (2 times). The exposure for small intestine, lung, and spleen were equivalent to that for plasma. Organs with lower levels of exposure were the heart (0.3 times that for plasma), thigh (0.2 times), and brain (0.06 times). Kinetically, drug was cleared more slowly from all tissues than from plasma, indicating that terminal-phase equilibrium had not been achieved by 24 h. Thus, some measure of accumulation is predicted for all tissues. Single daily doses of MK-0991 should provide adequate systemic levels of fungicidal activity as a result of its long half-life pharmacokinetics, wide distribution, and slowly

  4. A continuous dry 300 mK cooler for THz sensing applications.

    PubMed

    Klemencic, G M; Ade, P A R; Chase, S; Sudiwala, R; Woodcraft, A L

    2016-04-01

    We describe and demonstrate the automated operation of a novel cryostat design that is capable of maintaining an unloaded base temperature of less than 300 mK continuously, without the need to recycle the gases within the final cold head, as is the case for conventional single shot sorption pumped (3)He cooling systems. This closed dry system uses only 5 l of (3)He gas, making this an economical alternative to traditional systems where a long hold time is required. During testing, a temperature of 365 mK was maintained with a constant 20 μW load, simulating the cooling requirement of a far infrared camera. PMID:27131707

  5. On-Chip ESR Measurements of DPPH at mK Temperatures

    NASA Astrophysics Data System (ADS)

    Voesch, Wolfgang; Thiemann, Markus; Bothner, Daniel; Dressel, Martin; Scheffler, Marc

    We study electron spin resonance (ESR) of DPPH at mK temperatures. Here we employ a superconducting coplanar microwave resonator that allows convenient implementation in a dilution refrigerator as well as operation at multiple ESR frequencies, in this case 1.5 GHz, 3.0 GHz, and 4.5 GHz. We find a strong temperature and magnetic field dependence of the ESR of DPPH below 1 K, which is consistent with an antiferromagnetic transition. Our study documents the potential of this on-chip ESR technique for mK experiments and elucidates the possibility to use DPPH as an ESR reference material in this regime.

  6. Stretched Inertial Jets

    NASA Astrophysics Data System (ADS)

    Ghabache, Elisabeth; Antkowiak, Arnaud; Seon, Thomas; Villermaux, Emmanuel

    2015-11-01

    Liquid jets often arise as short-lived bursting liquid flows. Cavitation or impact-driven jets, bursting champagne bubbles, shaped-charge jets, ballistospores or drop-on-demand inkjet printing are a few examples where liquid jets are suddenly released. The trademark of all these discharge jets is the property of being stretched, due to the quenching injection. the present theoretical and experimental investigation, the structure of the jet flow field will be unraveled experimentally for a few emblematic occurrences of discharge jets. Though the injection markedly depends on each flow configuration, the jet velocity field will be shown to be systematically and rapidly attracted to the universal stretching flow z/t. The emergence of this inertial attractor actually only relies on simple kinematic ingredients, and as such is fairly generic. The universality of the jet velocity structure will be discussed.

  7. Electric jets following the occurrence of sprites

    NASA Astrophysics Data System (ADS)

    Lee, L.; Chou, J.; Huang, S.; Chang, S.; Wu, Y.; Lee, Y.; Kuo, C.; Chen, A. B.; Su, H.; Hsu, R.; Frey, H. U.; Mende, S. B.; Takahashi, Y.; Lee, L.

    2010-12-01

    Sprites are discharges occurring at the altitudes ~40 to 90 km, which are usually associated with positive cloud-to-ground lightning (+CGs). Electric jets, which include blue jets (BJs) with the terminal altitude of ~40km and gigantic jets (GJs) emanating to the lower ionosphere, are upward discharges from the cloud tops toward the upper atmosphere. From previous ground observations, it has been reported that the secondary discharges (“palm-tree” [Heavner, 2000] or “sprite-initiated secondary TLEs” [Marshall and Inan, 2007]) following sprites occurred in altitudes between the cloud top and the bottom of the sprite. From July 2004 to June 2010, ISUAL has recorded dozens of events which resemble the secondary TLEs. From image and photometric data recorded by ISUAL, all these secondary TLEs have the characteristics of jets, so we call these events “secondary jets”. These secondary jets are categorized into two groups according to their emanating horizontal positions in relative to the sprites. Group-I secondary jets occurred in the cloud top region which is directly below the sprites. The terminal altitude is ~ 40-50km for most of group-I secondary jets. Several group-I secondary jets appear to originate from the cloud top region below the symmetric center of the clustering sprites and then propagate toward the lower ionosphere. While the group-II secondary jets originate from region outside the shielding area of the clustering sprites. In this paper, the image and the photometric characteristics of the secondary jets will be presented and the possible generating mechanisms will be discussed.

  8. A Coronal Hole Jet Observed with Hinode and the Solar Dynamics Observatory

    NASA Technical Reports Server (NTRS)

    Young, Peter H.; Muglach, Karin

    2014-01-01

    A small blowout jet was observed at the boundary of the south coronal hole on 2011 February 8 at around 21:00 UT. Images from the Atmospheric Imaging Assembly (AIA) on board the Solar Dynamics Observatory (SDO) revealed an expanding loop rising from one footpoint of a compact, bipolar bright point. Magnetograms from the Helioseismic Magnetic Imager (HMI) on board SDO showed that the jet was triggered by the cancelation of a parasitic positive polarity feature near the negative pole of the bright point. The jet emission was present for 25 mins and it extended 30 Mm from the bright point. Spectra from the EUV Imaging Spectrometer on board Hinode yielded a temperature and density of 1.6 MK and 0.9-1.7 × 10( exp 8) cu cm for the ejected plasma. Line-of-sight velocities reached up to 250 km/s. The density of the bright point was 7.6 × 10(exp 8) cu cm, and the peak of the bright point's emission measure occurred at 1.3 MK, with no plasma above 3 MK.

  9. Plasma Heating to Super-Hot Temperatures (>30 MK) in the August 9, 2011 Solar Flare

    NASA Astrophysics Data System (ADS)

    Sharykin, Ivan; Struminsky, Alexei; Zimovets, Ivan

    2015-08-01

    We investigate the August 9, 2011 solar flare of X-ray class X6.9, the "hottest" flare from 2000 to 2012, with a peak plasma temperature according to GOES data of 33 MK. Our goal is to determine the cause of such an anomalously high plasma temperature and to investigate the energy balance in the flare region with allowance made for the presence of a super-hot plasma (>30 MK). We analyze the RHESSI, GOES, AIA/SDO, and EVE/SDO data and discuss the spatial structure of the flare region and the results of our spectral analysis of its X-ray emission. Our analysis of the RHESSI X-ray spectra is performed in the one-temperature and two-temperature approximations by taking into account the emission of hot (20 MK) and super-hot (45 MK) plasmas. The hard X-ray spectrum in both models is fitted by power laws. The observed peculiarities of the flare are shown to be better explained in terms of the two-temperature model, in which the super-hot plasma is located at the flare loop tops (or in the magnetic cusp region). The formation of the super-hot plasma can be associated with its heating through primary energy release and with the suppression of thermal conduction.

  10. Au-Ge film thermometers for temperature range 30 mK-300 K

    NASA Astrophysics Data System (ADS)

    Béthoux, O.; Brusetti, R.; Lasjaunias, J. C.; Sahling, S.

    After optmization of the Au concentration and the annealing temperature, highly sensitive Au-Ge film thermometers for the temperature range 30 mK-300 K were obtained. The thermometers show good reproducibility, a very short relaxation time constant at low temperatures and are quite insensitive to magnetic field.

  11. Discovery of MK-8718, an HIV Protease Inhibitor Containing a Novel Morpholine Aspartate Binding Group.

    PubMed

    Bungard, Christopher J; Williams, Peter D; Ballard, Jeanine E; Bennett, David J; Beaulieu, Christian; Bahnck-Teets, Carolyn; Carroll, Steve S; Chang, Ronald K; Dubost, David C; Fay, John F; Diamond, Tracy L; Greshock, Thomas J; Hao, Li; Holloway, M Katharine; Felock, Peter J; Gesell, Jennifer J; Su, Hua-Poo; Manikowski, Jesse J; McKay, Daniel J; Miller, Mike; Min, Xu; Molinaro, Carmela; Moradei, Oscar M; Nantermet, Philippe G; Nadeau, Christian; Sanchez, Rosa I; Satyanarayana, Tummanapalli; Shipe, William D; Singh, Sanjay K; Truong, Vouy Linh; Vijayasaradhi, Sivalenka; Wiscount, Catherine M; Vacca, Joseph P; Crane, Sheldon N; McCauley, John A

    2016-07-14

    A novel HIV protease inhibitor was designed using a morpholine core as the aspartate binding group. Analysis of the crystal structure of the initial lead bound to HIV protease enabled optimization of enzyme potency and antiviral activity. This afforded a series of potent orally bioavailable inhibitors of which MK-8718 was identified as a compound with a favorable overall profile. PMID:27437081

  12. MK3 controls Polycomb target gene expression via negative feedback on ERK

    PubMed Central

    2012-01-01

    Background Gene-environment interactions are mediated by epigenetic mechanisms. Polycomb Group proteins constitute part of an epigenetic cellular transcriptional memory system that is subject to dynamic modulation during differentiation. Molecular insight in processes that control dynamic chromatin association and dissociation of Polycomb repressive complexes during and beyond development is limited. We recently showed that MK3 interacts with Polycomb repressive complex 1 (PRC1). The functional relevance of this interaction, however, remained poorly understood. MK3 is activated downstream of mitogen- and stress-activated protein kinases (M/SAPKs), all of which fulfill crucial roles during development. We here use activation of the immediate-early response gene ATF3, a bona fide PRC1 target gene, as a model to study how MK3 and its effector kinases MAPK/ERK and SAPK/P38 are involved in regulation of PRC1-dependent ATF3 transcription. Results Our current data show that mitogenic signaling through ERK, P38 and MK3 regulates ATF3 expression by PRC1/chromatin dissociation and epigenetic modulation. Mitogenic stimulation results in transient P38-dependent H3S28 phosphorylation and ERK-driven PRC1/chromatin dissociation at PRC1 targets. H3S28 phosphorylation by itself appears not sufficient to induce PRC1/chromatin dissociation, nor ATF3 transcription, as inhibition of MEK/ERK signaling blocks BMI1/chromatin dissociation and ATF3 expression, despite induced H3S28 phosphorylation. In addition, we establish that concomitant loss of local H3K27me3 promoter marking is not required for ATF3 activation. We identify pERK as a novel signaling-induced binding partner of PRC1, and provide evidence that MK3 controls ATF3 expression in cultured cells via negative regulatory feedback on M/SAPKs. Dramatically increased ectopic wing vein formation in the absence of Drosophila MK in a Drosophila ERK gain-of-function wing vein patterning model, supports the existence of MK

  13. NASA Jet Noise Research

    NASA Technical Reports Server (NTRS)

    Henderson, Brenda

    2012-01-01

    The presentation highlights jet-noise research conducted in the Subsonic Fixed Wing, Supersonics, and Environmentally Responsible Aviation Projects in the Fundamental Aeronautics Program at NASA. The research efforts discussed include NASA's updated Aircraft NOise Prediction Program (ANOPP2), acoustic-analogy-based prediction tools, jet-surface-interaction studies, plasma-actuator investigations, N+2 Supersonics Validation studies, rectangular-jet experiments, twin-jet experiments, and Hybrid Wind Body (HWB) activities.

  14. Jets of incipient liquids

    NASA Astrophysics Data System (ADS)

    Reshetnikov, A. V.; Mazheiko, N. A.; Skripov, V. P.

    2000-05-01

    Jets of incipient water escaping into the atmosphere through a short channel are photographed. In some experiments. complete disintegration of the jet is observed. The relationship of this phenomenon with intense volume incipience is considered. The role of the Coanda effect upon complete opening of the jet is revealed. Measurement results of the recoil force R of the jets of incipient liquids are presented. Cases of negative thrust caused by the Coanda effect are noted. Generalization of experimental data is proposed.

  15. Study of Jet Transverse Momentum and Jet Rapidity Dependence on Dijet Azimuthal Decorrelations

    SciTech Connect

    Chakravarthula, Kiran

    2012-01-01

    In a collision experiment involving highly energetic particles such as hadrons, processes at high momentum transfers can provide information useful for many studies involving Quantum Chromodynamics (QCD). One way of analyzing these interactions is through angular distributions. In hadron-hadron collisions, the angular distribution between the two leading jets with the largest transverse momentum (pT ) is affected by the production of additional jets. While soft radiation causes small differences in the azimuthal angular distribution of the two leading jets produced in a collision event, additional hard jets produced in the event have more pronounced influence on the distribution of the two leading jets produced in the collision. Thus, the dijet azimuthal angular distribution can serve as a variable that can be used to study the transition from soft to hard QCD processes in a collision event. This dissertation presents a triple-differential study involving the azimuthal angular distribution and the jet transverse momenta, and jet rapidities of the first two leading jets. The data used for this research are obtained from proton-antiproton (p$\\bar{p}$) collisions occurring at a center of mass energy of 1.96TeV, using the DØ detector in Run II of the Tevatron Collider at the Fermi National Accelerator Laboratory (FNAL) in Illinois, USA. Comparisons are made to perturbative QCD (pQCD) predictions at next-to-leading order (NLO).

  16. Magnetic jets in long GRBs: jet stability, energy dissipation & the connection with the magnetar model

    NASA Astrophysics Data System (ADS)

    Bromberg, Omer

    2016-07-01

    It is commonly accepted that jets in long GRBs are powered by the magnetized rotation of a compact object: a BH or a fastly rotating magnetar. Such jets are intrinsically unstable to disruptive kink modes, yet they maintain their shape over many orders of magnitude as they propagate through the star and beyond, while converting their electromagnetic energy into radiation and kinetic energy. This calls for an efficient dissipation mechanism to work within the jet, without causing its disruption. In this talk I will present results from a 3D study of relativistic magnetized GRB jets propagating in stellar envelopes. The collimation of the jet leads to two types of instabilities: i) a local kink mode that causes internal dissipation of the magnetic energy to a state of equipartition with the thermal energy, ii) a global kink mode, which bodily deforms the jet, causing it to slow down may lead to jet stalling. I will discuss the interesting implications from these results on the energy emission in long GRBs and on the type of compact objects that power them. In particular I will show that within the framework of the magnetar model, the jet is expected to become highly kinked unstable and fail to breakout of the star. Instead it inflates a bubble with ~10^52 erg of energy at the center of the star leading to a highly energetic supernova.

  17. Measurement of sigma(p anti-p -> t anti-t) in the tau + jets channel by the D� experiment at Run II of the Tevatron Collider

    SciTech Connect

    Arov, Mikhail; /Northern Illinois U.

    2008-07-01

    The top quark is the heaviest and most mysterious of the known elementary particles. Therefore, careful study of its production rate and other properties is of utmost importance for modern particle physics. The Tevatron is the only facility currently capable of studying top quark properties by on-shell production. Measurement of the top quark pair production cross section is one of the major goals of the Tevatron Run II physics program. It provides an excellent test of QCD at energies exceeding 100 GeV. We report on a new measurement of p{bar p} {yields} t{bar t} production at {radical} = 1.96 TeV using 350 pb{sup -1} of data collected with the D0 detector between 2002 and 2005. We focus on the final state where a W boson from one of the top quarks decays into a {tau} lepton and its associated neutrino, while the other decays into a quark-antiquark pair. We aim to select those events in which the {tau} lepton subsequently decays to one or three charged hadrons, zero or more neutral hadrons and a tau neutrino (the charge conjugate processes are implied in all of the above). The observable signature thus consists of a narrow calorimeter shower with associated track(s) characteristic of a hadronic tau decay, four or more jets, of which two are initiated by b quarks accompanying the W's in the top quark decays, and a large net missing momentum in the transverse plane due to the energetic neutrino-antineutrino pair that leave no trace in the detector media. The preliminary result for the measured cross section is: {sigma}(t{bar t}) = 5.1{sub -3.5}{sup +4.3}(stat){sub -0.7}{sup +0.7}(syst) {+-} 0.3 (lumi.) pb.

  18. Oxytocin reversed MK-801-induced social interaction and aggression deficits in zebrafish.

    PubMed

    Zimmermann, Fernanda Francine; Gaspary, Karina Vidarte; Siebel, Anna Maria; Bonan, Carla Denise

    2016-09-15

    Changes in social behavior occur in several neuropsychiatric disorders such as schizophrenia and autism. The interaction between individuals is an essential aspect and an adaptive response of several species, among them the zebrafish. Oxytocin is a neuroendocrine hormone associated with social behavior. The aim of the present study was to investigate the effects of MK-801, a non-competitive antagonist of glutamate NMDA receptors, on social interaction and aggression in zebrafish. We also examined the modulation of those effects by oxytocin, the oxytocin receptor agonist carbetocin and the oxytocin receptor antagonist L-368,899. Our results showed that MK-801 induced a decrease in the time spent in the segment closest to the conspecific school and in the time spent in the segment nearest to the mirror image, suggesting an effect on social behavior. The treatment with oxytocin after the exposure to MK-801 was able to reestablish the time spent in the segment closest to the conspecific school, as well as the time spent in the segment nearest to the mirror image. In addition, in support of the role of the oxytocin pathway in modulating those responses, we showed that the oxytocin receptor agonist carbetocin reestablished the social and aggressive behavioral deficits induced by MK-801. However, the oxytocin receptor antagonist L-368,899 was not able to reverse the behavioral changes induced by MK-801. This study supports the critical role for NMDA receptors and the oxytocinergic system in the regulation of social behavior and aggression which may be relevant for the mechanisms associated to autism and schizophrenia. PMID:27247142

  19. DNA microarray unravels rapid changes in transcriptome of MK-801 treated rat brain

    PubMed Central

    Kobayashi, Yuka; Kulikova, Sofya P; Shibato, Junko; Rakwal, Randeep; Satoh, Hiroyuki; Pinault, Didier; Masuo, Yoshinori

    2015-01-01

    AIM: To investigate the impact of MK-801 on gene expression patterns genome wide in rat brain regions. METHODS: Rats were treated with an intraperitoneal injection of MK-801 [0.08 (low-dose) and 0.16 (high-dose) mg/kg] or NaCl (vehicle control). In a first series of experiment, the frontoparietal electrocorticogram was recorded 15 min before and 60 min after injection. In a second series of experiments, the whole brain of each animal was rapidly removed at 40 min post-injection, and different regions were separated: amygdala, cerebral cortex, hippocampus, hypothalamus, midbrain and ventral striatum on ice followed by DNA microarray (4 × 44 K whole rat genome chip) analysis. RESULTS: Spectral analysis revealed that a single systemic injection of MK-801 significantly and selectively augmented the power of baseline gamma frequency (30-80 Hz) oscillations in the frontoparietal electroencephalogram. DNA microarray analysis showed the largest number (up- and down- regulations) of gene expressions in the cerebral cortex (378), midbrain (376), hippocampus (375), ventral striatum (353), amygdala (301), and hypothalamus (201) under low-dose (0.08 mg/kg) of MK-801. Under high-dose (0.16 mg/kg), ventral striatum (811) showed the largest number of gene expression changes. Gene expression changes were functionally categorized to reveal expression of genes and function varies with each brain region. CONCLUSION: Acute MK-801 treatment increases synchrony of baseline gamma oscillations, and causes very early changes in gene expressions in six individual rat brain regions, a first report. PMID:26629322

  20. Prewhirl Jet Model

    NASA Technical Reports Server (NTRS)

    Meng, S. Y.; Jensen, M.; Jackson, E. D.

    1985-01-01

    Simple accurate model of centrifugal or rocket engine pumps provides information necessary to design inducer backflow deflector, backflow eliminator and prewhirl jet in jet mixing zones. Jet design based on this model shows improvement in inducer suction performance and reduced cavitation damage.

  1. Deficiency of MAPK-activated protein kinase 2 (MK2) prevents adverse remodelling and promotes endothelial healing after arterial injury.

    PubMed

    Kapopara, P R; von Felden, J; Soehnlein, O; Wang, Y; Napp, L C; Sonnenschein, K; Wollert, K C; Schieffer, B; Gaestel, M; Bauersachs, J; Bavendiek, U

    2014-12-01

    Maladaptive remodelling of the arterial wall after mechanical injury (e. g. angioplasty) is characterised by inflammation, neointima formation and media hypertrophy, resulting in narrowing of the affected artery. Moreover, mechanical injury of the arterial wall causes loss of the vessel protecting endothelial cell monolayer. Mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MK2), a major downstream target of p38 MAPK, regulates inflammation, cell migration and proliferation, essential processes for vascular remodelling and re-endothelialisation. Therefore, we investigated the role of MK2 in remodelling and re-endothelialisation after arterial injury in genetically modified mice in vivo. Hypercholesterolaemic low-density-lipoprotein-receptor-deficient mice (ldlr-/-) were subjected to wire injury of the common carotid artery. MK2-deficiency (ldlr-/-/mk2-/-) nearly completely prevented neointima formation, media hypertrophy, and lumen loss after injury. This was accompanied by reduced proliferation and migration of MK2-deficient smooth muscle cells. In addition, MK2-deficiency severely reduced monocyte adhesion to the arterial wall (day 3 after injury, intravital microscopy), which may be attributed to reduced expression of the chemokine ligands CCL2 and CCL5. In line, MK2-deficiency significantly reduced the content of monocytes, neutrophiles and lymphocytes of the arterial wall (day 7 after injury, flow cytometry). In conclusion, in a model of endothelial injury (electric injury), MK2-deficiency strongly increased proliferation of endothelial cells and improved re-endothelialisation of the arterial wall after injury. Deficiency of MK2 prevents adverse remodelling and promotes endothelial healing of the arterial wall after injury, suggesting that MK2-inhibition is a very attractive intervention to prevent restenosis after percutaneous therapeutic angioplasty. PMID:25120198

  2. New antineoplastic agent, MK615, from UME (a Variety of) Japanese apricot inhibits growth of breast cancer cells in vitro.

    PubMed

    Nakagawa, Aya; Sawada, Tokihiko; Okada, Toshie; Ohsawa, Tatsushi; Adachi, Masakazu; Kubota, Keiichi

    2007-01-01

    MK615 is an extract mixture containing hydrophobic substances from Japanese apricot. In this study, the antineoplastic effects of MK615 against breast cancer cells were investigated. Two breast cancer cell lines, MDA-MB-468 (MDA) and MCF7, were cultured with (600, 300, and 150 mug/mL) or without MK615. After 72 hours of incubation, growth inhibition was evaluated by MTT assay. The cells were then cultured with MK615 (300 mug/mL) and morphological changes were studied by light and electron microscopy. Finally, the mechanism of the antineoplastic effect of MK615 was evaluated by cell cycle and apoptosis assay. MK615 inhibited the growth of MDA and MCF7 in a dose-dependent manner. The percentage growth inhibition of MDA at dosages of 600, 300, and 150 mug/mL was 59.2%, 52.4%, and 23.3%, respectively, and that for MCF7 was 83.5%, 52.7%, and 16.6%, respectively. Morphological changes after MK615 treatment included massive vacuolization in the cytoplasm and apoptotic changes in the nucleus. These changes began to be apparent after at least 6 hours of incubation. Cell cycle analysis showed that MK615 increased the proportion of cells in the G2-M phase in both MDA (7.8-11.7%) and MCF7 (8.1-18.7%), and finally both cell lines became apoptotic. The proportion of apoptotic cells increased with incubation time. MK615 effectively inhibits the growth of breast cancer cells in vitro, possibly by cell cycle modification and apotosis induction. MK615 should be further investigated as a promising anti-breast cancer agent. PMID:17214792

  3. Aeroacoustics of hot jets

    NASA Astrophysics Data System (ADS)

    Viswanathan, K.

    2004-10-01

    A systematic study has been undertaken to quantify the effect of jet temperature on the noise radiated by subsonic jets. Nozzles of different diameters were tested to uncover the effects of Reynolds number. All the tests were carried out at Boeing's Low Speed Aeroacoustic Facility, with simultaneous measurement of thrust and noise. It is concluded that the change in spectral shape at high jet temperatures, normally attributed to the contribution from dipoles, is due to Reynolds number effects and not dipoles. This effect has not been identified before. A critical value of the Reynolds number that would need to be maintained to avoid the effects associated with low Reynolds number has been estimated to be {˜}400 000. It is well-known that large-scale structures are the dominant generators of noise in the peak radiation direction for high-speed jets. Experimental evidence is presented that shows the spectral shape at angles close to the jet axis from unheated low subsonic jets to be the same as from heated supersonic jets. A possible mechanism for the observed trend is proposed. When a subsonic jet is heated with the Mach number held constant, there is a broadening of the angular sector in which peak radiation occurs. Furthermore, there is a broadening of the spectral peak. Similar trends have been observed at supersonic Mach numbers. The spectral shapes in the forward quadrant and in the near-normal angles from unheated and heated subsonic jets also conform to the universal shape obtained from supersonic jet data. Just as for unheated jets, the peak frequency at angles close to the jet axis is independent of jet velocity as long as the acoustic Mach number is less than unity. The extensive database generated in the current test programme is intended to provide test cases with high-quality data that could be used for the evaluation of theoretical/semi-theoretical jet noise prediction methodologies.

  4. Z boson production in association with heavy quark jets at D0

    SciTech Connect

    Joseph Anthony Zennamo, III

    2013-10-28

    The dominant background in searches for a Higgs boson decaying into b-quarks at the Tevatron is production of a Z boson in association with either b- or c-quark initiated jets (b or c jets). This thesis describes the first measurements of the ratio of differential cross sections σ (Z + b jet)/ σ(Z + jet), and the first measurements of the ratio of cross sections σ (Z + c jet)/ σ(Z + jet) and σ (Z + c jet)/ σ(Z + b jet). These measurements are performed using the full D0 Run II data set corresponding to an integrated luminosity of 9.7 fb-1. The ratio of differential cross sections σ(Z + b jet)/σ (Z + jet) have been measured as a function of jet and Z boson pT , jet η , and Δφ(Z, jet). The Z+c jet ratios of differential cross sections are measured as a function of jet and Z boson pT .

  5. A search for ionized jets towards massive young stellar objects

    NASA Astrophysics Data System (ADS)

    Purser, S. J. D.; Lumsden, S. L.; Hoare, M. G.; Urquhart, J. S.; Cunningham, N.; Purcell, C. R.; Brooks, K. J.; Garay, G.; Gúzman, A. E.; Voronkov, M. A.

    2016-07-01

    Radio continuum observations using the Australia telescope compact array at 5.5, 9.0, 17.0 and 22.8 GHz have detected free-free emission associated with 45 of 49 massive young stellar objects and H II regions. Of these, 26 sources are classified as ionized jets (12 of which are candidates), 2 as ambiguous jets or disc winds, 1 as a disc-wind, 14 as H II regions and 2 were unable to be categorized. Classification as ionized jets is based upon morphology, radio flux and spectral index, in conjunction with previous observational results at other wavelengths. Radio luminosity and momentum are found to scale with bolometric luminosity in the same way as low-mass jets, indicating a common mechanism for jet production across all masses. In 13 of the jets, we see associated non-thermal/optically thin lobes resulting from shocks either internal to the jet and/or at working surfaces. 10 jets display non-thermal (synchrotron emission) spectra in their lobes, with an average spectral index of α = -0.55 consistent with Fermi acceleration in shocks. This shows that magnetic fields are present, in agreement with models of jet formation incorporating magnetic fields. Since the production of collimated radio jets is associated with accretion processes, the results presented in this paper support the picture of disc-mediated accretion for the formation of massive stars with an upper limit on the jet phase lasting approximately 6.5 × 104 yr. Typical mass-loss rates in the jet are found to be 1.4 × 10-5 M⊙ yr-1 with associated momentum rates of the order of (1-2) × 10-2 M⊙ km s-1 yr-1.

  6. Causality and stability of cosmic jets

    NASA Astrophysics Data System (ADS)

    Porth, Oliver; Komissarov, Serguei S.

    2015-09-01

    In stark contrast to their laboratory and terrestrial counterparts, cosmic jets appear to be very stable. They are able to penetrate vast spaces, which exceed by up to a billion times the size of their central engines. We propose that the reason behind this remarkable property is the loss of causal connectivity across these jets, caused by their rapid expansion in response to fast decline of external pressure with the distance from the `jet engine'. In atmospheres with power-law pressure distribution, pext ∝ z-κ, the total loss of causal connectivity occurs, when κ > 2 - the steepness which is expected to be quite common for many astrophysical environments. This conclusion does not seem to depend on the physical nature of jets - it applies both to relativistic and non-relativistic flows, both magnetically dominated and unmagnetized jets. In order to verify it, we have carried out numerical simulations of moderately magnetized and moderately relativistic jets. The results give strong support to our hypothesis and provide with valuable insights. In particular, we find that the z-pinched inner cores of magnetic jets expand slower than their envelopes and become susceptible to instabilities even when the whole jet is stable. This may result in local dissipation and emission without global disintegration of the flow. Cosmic jets may become globally unstable when they enter flat sections of external atmospheres. We propose that the Fanaroff-Riley (FR) morphological division of extragalactic radio sources into two classes is related to this issue. In particular, we argue that the low power FR-I jets become reconfined, causally connected and globally unstable on the scale of galactic X-ray coronas, whereas more powerful FR-II jets reconfine much further out, already on the scale of radio lobes and remain largely intact until they terminate at hotspots. Using this idea, we derived the relationship between the critical jet power and the optical luminosity of the host

  7. Theory of laminar viscous jets

    NASA Astrophysics Data System (ADS)

    Martynenko, O. G.; Korovkin, V. N.; Sokovishin, Iu. A.

    Results of recent theoretical studies of laminar jet flows of a viscous incompressible fluid are reviewed. In particular, attention is given to plane, fan-shaped, axisymmetric, and swirling jet flows; jet flows behind bodies; and slipstream jet flows. The discussion also covers dissipation of mechanical energy in jet flows, jet flows with a zero excess momentum, and asymptotic series expansions in the theory of jet flows.

  8. Evaluation of the ocean technology system's MK 1-S wireless surface unit, MK 1 DCI two diver air radio, MK 1-D-A wireless diver unit, MK 1-D-H hardwire diver unit and MK 1-D/S unit

    NASA Astrophysics Data System (ADS)

    Reyle, B. E.

    1985-05-01

    The OTS was evaluated for intelligibility, reliability and human engineering. The test subjects possessed various levels of experience with wireless or hardwire communication and SCUBA. The conditions under which the equipment was tested varied. Surface air temperatures ranged from 37 to 85 F; water temperatures from 65 to 80 F; and water depths from 8 feet of seawater (FSW) to greater than 60 FSW. The tests were conducted inside a circular 30 foot deep ascent tower, in shallow open bay water, and finally in an open ocean environment. The equipment was evaluated in areas of both high and low noise levels on the surface as well as in water. The OTS produced an overall intelligibility of 89.24% during manned open water testing using the Modified Rhyme Test (MRT) as the evaluation criteria, with a minimum effective range of 330 yards at 12 FSW and at least 875 yards, although it appears that the range of the MK 1-D-A is somewhat greater. Human engineering aspects of the OTS were found to be more than satisfactory, with no material failures encountered during testing. It is interesting to note that whenever the equipment required minor adjustments, these could be effected by the diver in the water (on the surface) and in most cases in less than 5 minutes. The amount of maintenance required on the OTS was minimal.

  9. Search for WW and WZ production in lepton, neutrino plus jets final states at CDF Run II and Silicon module production and detector control system for the ATLAS SemiConductor Tracker

    SciTech Connect

    Sfyrla, Anna; /Geneva U.

    2008-03-01

    In the first part of this work, we present a search for WW and WZ production in charged lepton, neutrino plus jets final states produced in p{bar p} collisions with {radical}s = 1.96 TeV at the Fermilab Tevatron, using 1.2 fb{sup -1} of data accumulated with the CDF II detector. This channel is yet to be observed in hadron colliders due to the large singleWplus jets background. However, this decay mode has a much larger branching fraction than the cleaner fully leptonic mode making it more sensitive to anomalous triple gauge couplings that manifest themselves at higher transverse W momentum. Because the final state is topologically similar to associated production of a Higgs boson with a W, the techniques developed in this analysis are also applicable in that search. An Artificial Neural Network has been used for the event selection optimization. The theoretical prediction for the cross section is {sigma}{sub WW/WZ}{sup theory} x Br(W {yields} {ell}{nu}; W/Z {yields} jj) = 2.09 {+-} 0.14 pb. They measured N{sub Signal} = 410 {+-} 212(stat) {+-} 102(sys) signal events that correspond to a cross section {sigma}{sub WW/WZ} x Br(W {yields} {ell}{nu}; W/Z {yields} jj) = 1.47 {+-} 0.77(stat) {+-} 0.38(sys) pb. The 95% CL upper limit to the cross section is estimated to be {sigma} x Br(W {yields} {ell}{nu}; W/Z {yields} jj) < 2.88 pb. The second part of the present work is technical and concerns the ATLAS SemiConductor Tracker (SCT) assembly phase. Although technical, the work in the SCT assembly phase is of prime importance for the good performance of the detector during data taking. The production at the University of Geneva of approximately one third of the silicon microstrip end-cap modules is presented. This collaborative effort of the university of Geneva group that lasted two years, resulted in 655 produced modules, 97% of which were good modules, constructed within the mechanical and electrical specifications and delivered in the SCT collaboration for assembly on

  10. MMI-0100 inhibits cardiac fibrosis in myocardial infarction by direct actions on cardiomyocytes and fibroblasts via MK2 inhibition

    PubMed Central

    Xu, Lei; Yates, Cecelia C.; Lockyer, Pamela; Xie, Liang; Bevilacqua, Ariana; He, Jun; Lander, Cynthia; Patterson, Cam; Willis, Monte

    2014-01-01

    The cell-permeant peptide inhibitor of MAPKAP kinase 2 (MK2), MMI-0100, inhibits MK2 and downstream fibrosis and inflammation. Recent studies have demonstrated that MMI-0100 reduces intimal hyperplasia in a mouse vein graft model, pulmonary fibrosis in a murine bleomycin-induced model and development of adhesions in conjunction with abdominal surgery. MK2 is critical to the pathogenesis of ischemic heart injury as MK2 −/− mice are resistant to ischemic remodeling. Therefore, we tested the hypothesis that inhibiting MK2 with MMI-0100 would protect the heart after acute myocardial infarction (AMI) in vivo. AMI was induced by placing a permanent LAD coronary ligation. When MMI-0100 peptide was given 30 minutes after permanent LAD coronary artery ligation, the resulting fibrosis was reduced/prevented ~50% at a 2 week time point, with a corresponding improvement in cardiac function and decrease in left ventricular dilation. In cultured cardiomyocytes and fibroblasts, MMI-0100 inhibited MK2 to reduce cardiomyocyte caspase 3/7 activity, while enhancing primary cardiac fibroblast caspase 3/7 activity, which may explain MMI-0100’s salvage of cardiac function and anti-fibrotic effects in vivo. These findings suggest that therapeutic inhibition of MK2 after acute MI, using rationally-designed cell-permeant peptides, inhibits cardiac fibrosis and maintains cardiac function by mechanisms that involve inhibiting cardiomyocyte apoptosis, while enhancing primary cardiac fibroblast cell death. PMID:25257914

  11. Targeting of Carbon Ion-Induced G2 Checkpoint Activation in Lung Cancer Cells Using Wee-1 Inhibitor MK-1775.

    PubMed

    Ma, Hongyu; Takahashi, Akihisa; Sejimo, Yukihiko; Adachi, Akiko; Kubo, Nobuteru; Isono, Mayu; Yoshida, Yukari; Kanai, Tatsuaki; Ohno, Tatsuya; Nakano, Takashi

    2015-12-01

    The potent inhibitor of the cell cycle checkpoint regulatory factor Wee-1, MK-1775, has been reported to enhance non-small cell lung cancer (NSCLC) cell sensitivity to photon radiation by abrogating radiation-induced G2 arrest. However, little is known about the effects of this sensitizer after exposure to carbon (C)-ion radiation. The purpose of this study was therefore to investigate the effects of C ions in combination with MK-1775 on the killing of NSCLC cells. Human NSCLC H1299 cells were exposed to X rays or C ions (290 MeV/n, 50 keV/μm at the center of a 6 cm spread-out Bragg peak) in the presence of MK-1775. The cell cycle was analyzed using flow cytometry and Western blotting. Radiosensitivity was determined using clonogenic survival assays. The mechanisms underlying MK-1775 radiosensitization were studied by observing H2AX phosphorylation and mitotic catastrophe. G2 checkpoint arrest was enhanced 2.3-fold by C-ion exposure compared with X-ray exposure. Radiation-induced G2 checkpoint arrest was abrogated by MK-1775. Exposure to radiation resulted in a significant reduction in the mitotic ratio and increased phosphorylation of cyclin-dependent kinase 1 (Cdk1), the primary downstream mediator of Wee-1-induced G2 arrest. The Wee-1 inhibitor, MK-1775 restored the mitotic ratio and suppressed Cdk1 phosphorylation. In addition, MK-1775 increased H1299 cell sensitivity to C ions and X rays independent of TP53 status. MK-1775 also significantly increased H2AX phosphorylation and mitotic catastrophe in irradiated cells. These results suggest that the G2 checkpoint inhibitor MK-1775 can enhance the sensitivity of human NSCLC cells to C ions as well as X rays. PMID:26645158

  12. Jet Substructure Without Trees

    SciTech Connect

    Jankowiak, Martin; Larkoski, Andrew J.; /SLAC /Stanford U., ITP

    2011-08-19

    We present an alternative approach to identifying and characterizing jet substructure. An angular correlation function is introduced that can be used to extract angular and mass scales within a jet without reference to a clustering algorithm. This procedure gives rise to a number of useful jet observables. As an application, we construct a top quark tagging algorithm that is competitive with existing methods. In preparation for the LHC, the past several years have seen extensive work on various aspects of collider searches. With the excellent resolution of the ATLAS and CMS detectors as a catalyst, one area that has undergone significant development is jet substructure physics. The use of jet substructure techniques, which probe the fine-grained details of how energy is distributed in jets, has two broad goals. First, measuring more than just the bulk properties of jets allows for additional probes of QCD. For example, jet substructure measurements can be compared against precision perturbative QCD calculations or used to tune Monte Carlo event generators. Second, jet substructure allows for additional handles in event discrimination. These handles could play an important role at the LHC in discriminating between signal and background events in a wide variety of particle searches. For example, Monte Carlo studies indicate that jet substructure techniques allow for efficient reconstruction of boosted heavy objects such as the W{sup {+-}} and Z{sup 0} gauge bosons, the top quark, and the Higgs boson.

  13. Monitoring Excitations of the N =1 Landau Level by Optical Emission at mK Temperatures

    NASA Astrophysics Data System (ADS)

    Levy, Antonio; Wurstbauer, Ursula; Fields, Dov; Pinczuk, Aron; Watson, John; Mondal, Sumit; Manfra, Michael J.; West, Ken W.; Pfeiffer, Loren N.

    2013-03-01

    Optical emission experiments have proven to be powerful contactless probe of collective states of electrons in the second (N =1) Landau Level (LL). We report the emission spectrum from optical recombination in the N =0 and N =1 LL's the second LL. The 2DEG is confined in ultra-high-mobility GaAs quantum well structures. Optical emission red-shifted from the main luminescence of the N =0 and N =1 LL are interpreted as shakeup processes of quasiparticles in the N =1 LL. Results of two samples with different carrier densities measured in the temperature range of 42mK <=T <=650mK will be compared. The experimental observations will be discussed taking into account the striking quantum phases dominating the second LL. Supported by NSF and AvH

  14. Targeting p38 or MK2 Enhances the Anti-Leukemic Activity of Smac-Mimetics.

    PubMed

    Lalaoui, Najoua; Hänggi, Kay; Brumatti, Gabriela; Chau, Diep; Nguyen, Nhu-Y N; Vasilikos, Lazaros; Spilgies, Lisanne M; Heckmann, Denise A; Ma, Chunyan; Ghisi, Margherita; Salmon, Jessica M; Matthews, Geoffrey M; de Valle, Elisha; Moujalled, Donia M; Menon, Manoj B; Spall, Sukhdeep Kaur; Glaser, Stefan P; Richmond, Jennifer; Lock, Richard B; Condon, Stephen M; Gugasyan, Raffi; Gaestel, Matthias; Guthridge, Mark; Johnstone, Ricky W; Munoz, Lenka; Wei, Andrew; Ekert, Paul G; Vaux, David L; Wong, W Wei-Lynn; Silke, John

    2016-02-01

    Birinapant is a smac-mimetic (SM) in clinical trials for treating cancer. SM antagonize inhibitor of apoptosis (IAP) proteins and simultaneously induce tumor necrosis factor (TNF) secretion to render cancers sensitive to TNF-induced killing. To enhance SM efficacy, we screened kinase inhibitors for their ability to increase TNF production of SM-treated cells. We showed that p38 inhibitors increased TNF induced by SM. Unexpectedly, even though p38 is required for Toll-like receptors to induce TNF, loss of p38 or its downstream kinase MK2 increased induction of TNF by SM. Hence, we show that the p38/MK2 axis can inhibit or promote TNF production, depending on the stimulus. Importantly, clinical p38 inhibitors overcame resistance of primary acute myeloid leukemia to birinapant. PMID:26859455

  15. Reflectometry measurements of 1/f noise in SQUID phase qubits at mK temperatures

    NASA Astrophysics Data System (ADS)

    Cooper, B. K.; Lewis, R. M.; Palmer, B. S.; Zaretskey, V.; Przybysz, A. J.; Kwon, H.; Anderson, J. R.; Lobb, C. J.; Wellstood, F. C.

    2009-03-01

    We measure 1/f noise spectra in dc SQUID phase qubits using a microwave reflectometry technique. One of the SQUID junctions is shunted by a large capacitor, forming a microwave frequency resonator biased and driven to show nonlinear response, typically at 1.5 GHz. This nonlinearity means small current or flux fluctuations produce large changes in reflected phase which we can measure using homodyne detection. Measurements from aluminum qubits on sapphire are compared to previous measurements of 1/f flux noise in SQUIDs and a similarly designed Nb/AlOx/Nb on silicon dc SQUID qubit fabricated by Hypres; data was taken at temperatures ranging from 50 mK to 500 mK.

  16. MK Classification and Dynamical Masses for Late-Type Visual Binaries

    NASA Astrophysics Data System (ADS)

    Tamazian, Vakhtang S.; Docobo, José A.; Melikian, Norair D.; Karapetian, Arthur A.

    2006-06-01

    On the basis of slit spectra obtained with the SCORPIO spectral camera attached to the 2.6 m telescope of the V. Ambartsumian Byurakan Astrophysical Observatory (Armenia), MK classifications for 30 visual binaries comprising mostly late K and M type stars are presented. Comparison with other determinations shows that this configuration provides a reliable MK classification. Dynamical masses for 25 systems are computed. Using standard mass-luminosity calibrations, individual mass sums for 11 pairs consisting of virtually single, nonvariable dwarfs are calculated, showing a good agreement with corresponding dynamical masses. The dynamical parallax of HIP 112354 is closer to the trigonometric parallax given in the Yale General Catalogue of Trigonometric Stellar Parallaxes (van Altena et al.) than to the Hipparcos parallax.

  17. On size tripartite Ramsey numbers of P3 versus mK1,n

    NASA Astrophysics Data System (ADS)

    Lusiani, Anie; Baskoro, Edy Tri; Saputro, Suhadi Wido

    2016-02-01

    Let Kl×t be a complete, balanced, multipartite graph consisting of l partite sets and t vertices in each partite set. For simple graphs G and H, the size multipartite Ramsey number mj (G, H) is the smallest natural number t such that any arbitrary red-blue coloring on the edges of Kl×t contains a red G or a blue H as a subgraph. In particular, if j = 3 then m3(G, H) is called the size tripartite Ramsey number of G and H. In this paper, we determine the exact values of the size tripartite numbers m3(P3, mK1,n) for all integers m ≥ 1 and n ≥ 3, where P3 is a path of order 3 and mK1,n is a disjoint union of m copies of a star K1,n.

  18. Thermoregulator and helium-level indicator using MK-64 pocket calculator

    SciTech Connect

    Bogdanov, S.V.; Donchenko, N.Ya.

    1987-08-01

    A thermoregulator for a helium cryostat and helium-level indicator are described that are based on an MK-64 programmable picket calculator with a built-in analog-digital converter. The temperature and helium-level sensors are TVO carbon resistors. The MK-64 makes it possible to maintain or change by program a temperature in the range of 1.2-77/sup 0/K with an accuracy of 0.05-0.5/sup 0/K and also to output to the calculator display and peripheral devices the temperature and any function of temperature. The calculator display indicates the presence of helium in the cryostat and the level of the helium tank.

  19. Sub-50-mK Electronic Cooling with Large-Area Superconducting Tunnel Junctions

    NASA Astrophysics Data System (ADS)

    Nguyen, H. Q.; Meschke, M.; Courtois, H.; Pekola, J. P.

    2014-11-01

    In electronic cooling with superconducting tunnel junctions, the cooling power is counterbalanced by the interaction with phonons and by the heat flow from the overheated leads. We study aluminum-based coolers that are equipped with a suspended normal metal and an efficient quasiparticle drain. At intermediate temperatures, the phonon bath of the suspended normal metal is cooled. By adjusting the junction transparency, we control the injection current and, thus, the temperature of the superconducting lead at the optimum cooling point. The best device shows remarkable cooling from 150 mK down to about 30 mK, a factor of 5 in temperature at a power of 40 pW. We discuss heat transport in our device and the reasons for cooling saturation at the low-temperature end.

  20. Hydrodynamics of pulsed jetting in juvenile and adult brief squid Lolliguncula brevis: evidence of multiple jet 'modes' and their implications for propulsive efficiency.

    PubMed

    Bartol, Ian K; Krueger, Paul S; Stewart, William J; Thompson, Joseph T

    2009-06-01

    The dynamics of pulsed jetting in squids throughout ontogeny is not well understood, especially with regard to the development of vortex rings, which are common features of mechanically generated jet pulses (also known as starting jets). Studies of mechanically generated starting jets have revealed a limiting principle for vortex ring formation characterized in terms of a ;formation number' (F), which delineates the transition between the formation of isolated vortex rings and vortex rings that have; pinched off' from the generating jet. Near F, there exists an optimum in pulse-averaged thrust with (potentially) low energetic cost, raising the question: do squids produce vortex rings and if so, do they fall near F, where propulsive benefits presumably occur? To better understand vortex ring dynamics and propulsive jet efficiency throughout ontogeny, brief squid Lolliguncula brevis ranging from 3.3 to 9.1 cm dorsal mantle length (DML) and swimming at speeds of 2.43-22.2 cms(-1) (0.54-3.50 DMLs(-1)) were studied using digital particle image velocimetry (DPIV). A range of jet structures were observed but most structures could be classified as variations of two principal jet modes: (1) jet mode I, where the ejected fluid rolled up into an isolated vortex ring; and (2) jet mode II, where the ejected fluid developed into a leading vortex ring that separated or ;pinched off' from a long trailing jet. The ratio of jet length [based on the vorticity extent (L(omega))] to jet diameter [based on peak vorticity locations (D(omega))] was <3.0 for jet mode I and >3.0 for jet mode II, placing the transition between modes in rough agreement with F determined in mechanical jet studies. Jet mode II produced greater time-averaged thrust and lift forces and was the jet mode most heavily used whereas jet mode I had higher propulsive efficiency, lower slip, shorter jet periods and a higher frequency of fin activity associated with it. No relationship between L(omega)/D(omega) and speed

  1. Upregulation of AKT3 Confers Resistance to the AKT Inhibitor MK2206 in Breast Cancer.

    PubMed

    Stottrup, Casey; Tsang, Tiffany; Chin, Y Rebecca

    2016-08-01

    Acquired resistance to molecular targeted therapy represents a major challenge for the effective treatment of cancer. Hyperactivation of the PI3K/AKT pathway is frequently observed in virtually all human malignancies, and numerous PI3K and AKT inhibitors are currently under clinical evaluation. However, mechanisms of acquired resistance to AKT inhibitors have yet to be described. Here, we use a breast cancer preclinical model to identify resistance mechanisms to a small molecule allosteric AKT inhibitor, MK2206. Using a step-wise and chronic high-dose exposure, breast cancer cell lines harboring oncogenic PI3K resistant to MK2206 were established. Using this model, we reveal that AKT3 expression is markedly upregulated in AKT inhibitor-resistant cells. Induction of AKT3 is regulated epigenetically by the bromodomain and extra terminal domain proteins. Importantly, knockdown of AKT3, but not AKT1 or AKT2, in resistant cells restores sensitivity to MK2206. AKT inhibitor-resistant cells also display an epithelial to mesenchymal transition phenotype as assessed by alterations in the levels of E-Cadherin, N-Cadherin, and vimentin, as well as enhanced invasiveness of tumor spheroids. Notably, the invasive morphology of resistant spheroids is diminished upon AKT3 depletion. We also show that resistance to MK2206 is reversible because upon drug removal resistant cells regain sensitivity to AKT inhibition, accompanied by reexpression of epithelial markers and reduction of AKT3 expression, implying that epigenetic reprogramming contributes to acquisition of resistance. These findings provide a rationale for developing therapeutics targeting AKT3 to circumvent acquired resistance in breast cancer. Mol Cancer Ther; 15(8); 1964-74. ©2016 AACR. PMID:27297869

  2. Structural and Thermodynamic Effects of Macrocyclization in HCV NS3/4A Inhibitor MK-5172.

    PubMed

    Soumana, Djadé I; Kurt Yilmaz, Nese; Prachanronarong, Kristina L; Aydin, Cihan; Ali, Akbar; Schiffer, Celia A

    2016-04-15

    Recent advances in direct-acting antivirals against Hepatitis C Virus (HCV) have led to the development of potent inhibitors, including MK-5172, that target the viral NS3/4A protease with relatively low susceptibility to resistance. MK-5172 has a P2-P4 macrocycle and a unique binding mode among current protease inhibitors where the P2 quinoxaline packs against the catalytic residues H57 and D81. However, the effect of macrocyclization on this binding mode is not clear, as is the relation between macrocyclization, thermodynamic stabilization, and susceptibility to the resistance mutation A156T. We have determined high-resolution crystal structures of linear and P1-P3 macrocyclic analogs of MK-5172 bound to WT and A156T protease and compared these structures, their molecular dynamics, and experimental binding thermodynamics to the parent compound. We find that the "unique" binding mode of MK-5172 is conserved even when the P2-P4 macrocycle is removed or replaced with a P1-P3 macrocycle. While beneficial to decreasing the entropic penalty associated with binding, the constraint exerted by the P2-P4 macrocycle prevents efficient rearrangement to accommodate the A156T mutation, a deficit alleviated in the linear and P1-P3 analogs. Design of macrocyclic inhibitors against NS3/4A needs to achieve the best balance between exerting optimal conformational constraint for enhancing potency, fitting within the substrate envelope and allowing adaptability to be robust against resistance mutations. PMID:26682473

  3. A 30 mK, 13.5 T scanning tunneling microscope with two independent tips.

    PubMed

    Roychowdhury, Anita; Gubrud, M A; Dana, R; Anderson, J R; Lobb, C J; Wellstood, F C; Dreyer, M

    2014-04-01

    We describe the design, construction, and performance of an ultra-low temperature, high-field scanning tunneling microscope (STM) with two independent tips. The STM is mounted on a dilution refrigerator and operates at a base temperature of 30 mK with magnetic fields of up to 13.5 T. We focus on the design of the two-tip STM head, as well as the sample transfer mechanism, which allows in situ transfer from an ultra high vacuum preparation chamber while the STM is at 1.5 K. Other design details such as the vibration isolation and rf-filtered wiring are also described. Their effectiveness is demonstrated via spectral current noise characteristics and the root mean square roughness of atomic resolution images. The high-field capability is shown by the magnetic field dependence of the superconducting gap of CuxBi2Se3. Finally, we present images and spectroscopy taken with superconducting Nb tips with the refrigerator at 35 mK that indicate that the effective temperature of our tips/sample is approximately 184 mK, corresponding to an energy resolution of 16 μeV. PMID:24784617

  4. A 30 mK, 13.5 T scanning tunneling microscope with two independent tips

    SciTech Connect

    Roychowdhury, Anita; Gubrud, M. A.; Dana, R.; Dreyer, M.; Anderson, J. R.; Lobb, C. J.; Wellstood, F. C.

    2014-04-15

    We describe the design, construction, and performance of an ultra-low temperature, high-field scanning tunneling microscope (STM) with two independent tips. The STM is mounted on a dilution refrigerator and operates at a base temperature of 30 mK with magnetic fields of up to 13.5 T. We focus on the design of the two-tip STM head, as well as the sample transfer mechanism, which allows in situ transfer from an ultra high vacuum preparation chamber while the STM is at 1.5 K. Other design details such as the vibration isolation and rf-filtered wiring are also described. Their effectiveness is demonstrated via spectral current noise characteristics and the root mean square roughness of atomic resolution images. The high-field capability is shown by the magnetic field dependence of the superconducting gap of Cu{sub x}Bi{sub 2}Se{sub 3}. Finally, we present images and spectroscopy taken with superconducting Nb tips with the refrigerator at 35 mK that indicate that the effective temperature of our tips/sample is approximately 184 mK, corresponding to an energy resolution of 16 μeV.

  5. Thrombin Maybe Plays an Important Role in MK Differentiation into Platelets

    PubMed Central

    Yang, Xiao-Lei; Ge, Meng-Kai; Mao, De-Kui; Lv, Ying-Tao; Sun, Shu-Yan; Yu, Ai-Ping

    2016-01-01

    Objectives. After development and differentiation, megakaryocytes (MKs) can produce platelets. As is well known, thrombopoietin (TPO) can induce MKs to differentiate. The effect of thrombin on MKs differentiation is not clear. In this study, we used a human megakaryoblastic leukemia cell line (Meg-01) to assess the effect of thrombin on MKs differentiation. Methods. In order to interrogate the role of thrombin in Meg-01 cells differentiation, the changes of morphology, cellular function, and expression of diverse factors were analyzed. Results. The results show that thrombin suppresses Meg-01 cells proliferation and induces apoptosis and cell cycle arrest. Thrombin upregulates the expression of CD41b, which is one of the most important MK markers. Globin transcription factor 1 (GATA-1), an important transcriptional regulator, controls MK development and maturation. The expression of GATA-1 is also upregulated by thrombin in Meg-01 cells. The expression of B-cell lymphoma 2 (Bcl-2), an apoptosis-inhibitory protein, is downregulated by thrombin. Phosphorylated protein kinase B (p-AKT) and phosphorylated extracellular signal-regulated kinase (p-ERK) were upregulated by thrombin in Meg-01 cells. All the results are consistent with Meg-01 cells treated with TPO. Discussion and Conclusion. In conclusion, all these data indicate that thrombin maybe plays an important role in MK differentiation into platelets. However, whether the platelet-like particles are certainly platelets remains unknown. PMID:27064425

  6. Effects of MK-801, ketamine and alaptide on quinolinate models in the maturing hippocampus.

    PubMed

    Keilhoff, G; Wolf, G; Stastný, F

    1991-01-01

    The ability of the N-methyl-D-aspartate receptor antagonists, MK-801, ketamine and alaptide [a newly synthesized cyclo(1-amino-1-cyclopentane-carbonyl-L-alanyl) with protective properties in models of hypoxia], to prevent neuronal degeneration caused by intracerebroventricular application of quinolinic acid was investigated. Neurodegenerative effects of quinolinate in the hippocampal formation were found to increase with the degree of maturity of glutamatergic target structures. A protective potency of the N-methyl-D-aspartate receptor antagonists was observed at all developmental stages studied (12- and 30-day-old and adult rats). MK-801 showed the highest efficacy, alaptide the lowest. These findings suggest a parallelism in maturity of glutamatergic transmission processes as one prerequisite of quinolinate vulnerability and postnatal increases of target fields of the protectives. Application of MK-801 or ketamine after quinolinate injection intensified their protective effects when compared to simultaneous or preadministration. This observation is interpreted as indicating that quinolinate is a prompter of a delayed neurodegenerative process rather than acting immediately as a toxicant. PMID:1832751

  7. Discovery of MK-8742: an HCV NS5A inhibitor with broad genotype activity.

    PubMed

    Coburn, Craig A; Meinke, Peter T; Chang, Wei; Fandozzi, Christine M; Graham, Donald J; Hu, Bin; Huang, Qian; Kargman, Stacia; Kozlowski, Joseph; Liu, Rong; McCauley, John A; Nomeir, Amin A; Soll, Richard M; Vacca, Joseph P; Wang, Dahai; Wu, Hao; Zhong, Bin; Olsen, David B; Ludmerer, Steven W

    2013-12-01

    The NS5A protein plays a critical role in the replication of HCV and has been the focus of numerous research efforts over the past few years. NS5A inhibitors have shown impressive in vitro potency profiles in HCV replicon assays, making them attractive components for inclusion in all oral combination regimens. Early work in the NS5A arena led to the discovery of our first clinical candidate, MK-4882 [2-((S)-pyrrolidin-2-yl)-5-(2-(4-(5-((S)-pyrrolidin-2-yl)-1H-imidazol-2-yl)phenyl)benzofuran-5-yl)-1H-imidazole]. While preclinical proof-of-concept studies in HCV-infected chimpanzees harboring chronic genotype 1 infections resulted in significant decreases in viral load after both single- and multiple-dose treatments, viral breakthrough proved to be a concern, thus necessitating the development of compounds with increased potency against a number of genotypes and NS5A resistance mutations. Modification of the MK-4882 core scaffold by introduction of a cyclic constraint afforded a series of tetracyclic inhibitors, which showed improved virologic profiles. Herein we describe the research efforts that led to the discovery of MK-8742, a tetracyclic indole-based NS5A inhibitor, which is currently in phase 2b clinical trials as part of an all-oral, interferon-free regimen for the treatment of HCV infection. PMID:24127258

  8. An Expert System for Classifying Stars on the MK Spectral Classification System

    NASA Astrophysics Data System (ADS)

    Corbally, Christopher J.; Gray, R. O.

    2013-01-01

    We will describe an expert computer system designed to classify stellar spectra on the MK Spectral Classification system employing methods similar to those of humans who make direct comparison with the MK classification standards. Like an expert human classifier, MKCLASS first comes up with a rough spectral type, and then refines that type by direct comparison with MK standards drawn from a standards library using spectral criteria appropriate to the spectral class. Certain common spectral-type peculiarities can also be detected by the program. The program is also capable of identifying WD spectra and carbon stars and giving appropriate (but currently approximate) spectral types on the relevant systems. We will show comparisons between spectral types (including luminosity types) performed by MKCLASS and humans. The program currently is capable of competent classifications in the violet-green region, but plans are underway to extend the spectral criteria into the red and near-infrared regions. Two standard libraries with resolutions of 1.8 and 3.6Å are now available, but a higher-resolution standard library, using the new spectrograph on the Vatican Advanced Technology Telescope, is currently under preparation. Once that library is available, MKCLASS and the spectral libraries will be made available to the astronomical community.

  9. An Expert Computer Program for Classifying Stars on the MK Spectral Classification System

    NASA Astrophysics Data System (ADS)

    Gray, R. O.; Corbally, C. J.

    2014-04-01

    This paper describes an expert computer program (MKCLASS) designed to classify stellar spectra on the MK Spectral Classification system in a way similar to humans—by direct comparison with the MK classification standards. Like an expert human classifier, the program first comes up with a rough spectral type, and then refines that spectral type by direct comparison with MK standards drawn from a standards library. A number of spectral peculiarities, including barium stars, Ap and Am stars, λ Bootis stars, carbon-rich giants, etc., can be detected and classified by the program. The program also evaluates the quality of the delivered spectral type. The program currently is capable of classifying spectra in the violet-green region in either the rectified or flux-calibrated format, although the accuracy of the flux calibration is not important. We report on tests of MKCLASS on spectra classified by human classifiers; those tests suggest that over the entire HR diagram, MKCLASS will classify in the temperature dimension with a precision of 0.6 spectral subclass, and in the luminosity dimension with a precision of about one half of a luminosity class. These results compare well with human classifiers.

  10. CRTH2 antagonist MK-7246: a synthetic evolution from discovery through development.

    PubMed

    Molinaro, Carmela; Bulger, Paul G; Lee, Ernest E; Kosjek, Birgit; Lau, Stephen; Gauvreau, Danny; Howard, Melissa E; Wallace, Debra J; O'Shea, Paul D

    2012-03-01

    In this paper, we report the development of different synthetic routes to MK-7246 (1) designed by the Process Chemistry group. The syntheses were initially designed as an enabling tool for Medicinal Chemistry colleagues in order to rapidly explore structure-activity relationships (SAR) and to procure the first milligrams of diverse target molecules for in vitro evaluation. The initial aziridine opening/cyclodehydration strategy was also directly amenable to the first GMP deliveries of MK-7246 (1), streamlining the transition from milligram to kilogram-scale production needed to support early preclinical and clinical evaluation of this compound. Subsequently a more scalable and cost-effective manufacturing route to MK-7246 (1) was engineered. Highlights of the manufacturing route include an Ir-catalyzed intramolecular N-H insertion of sulfoxonium ylide 41 and conversion of ketone 32 to amine 31 in a single step with excellent enantioselectivity through a transaminase process. Reactions such as these illustrate the enabling impact and efficiency gains that innovative developments in chemo- and biocatalysis can have on the synthesis of pharmaceutically relevant target molecules. PMID:22335767

  11. An expert computer program for classifying stars on the MK spectral classification system

    SciTech Connect

    Gray, R. O.; Corbally, C. J.

    2014-04-01

    This paper describes an expert computer program (MKCLASS) designed to classify stellar spectra on the MK Spectral Classification system in a way similar to humans—by direct comparison with the MK classification standards. Like an expert human classifier, the program first comes up with a rough spectral type, and then refines that spectral type by direct comparison with MK standards drawn from a standards library. A number of spectral peculiarities, including barium stars, Ap and Am stars, λ Bootis stars, carbon-rich giants, etc., can be detected and classified by the program. The program also evaluates the quality of the delivered spectral type. The program currently is capable of classifying spectra in the violet-green region in either the rectified or flux-calibrated format, although the accuracy of the flux calibration is not important. We report on tests of MKCLASS on spectra classified by human classifiers; those tests suggest that over the entire HR diagram, MKCLASS will classify in the temperature dimension with a precision of 0.6 spectral subclass, and in the luminosity dimension with a precision of about one half of a luminosity class. These results compare well with human classifiers.

  12. IR diagnostics of embedded jets: velocity resolved observations of the HH34 and HH1 jets

    NASA Astrophysics Data System (ADS)

    Garcia Lopez, R.; Nisini, B.; Giannini, T.; Eislöffel, J.; Bacciotti, F.; Podio, L.

    2008-09-01

    Context: We present VLT-ISAAC medium resolution spectroscopy of the HH34 and HH1 jets, driven by young Class 0/I sources. Aims: Our aim is to derive the kinematics and the physical parameters associated with infrared jets, and to study how they vary with jet velocity. Methods: We use several important diagnostic lines covered by our spectral range, such as [Fe II] 1.644~μm, 1.600~μm and H2 2.122~μm, to probe both the atomic and the molecular jet components. Results: In the inner jet region of HH34, we find that both the atomic and molecular gas present two components at high and low velocity (the so-called HVC and LVC), as reported by previous studies. The [Fe II] LVC in HH34 is detected up to large distances from the source (>1000 AU), at variance with TTauri jets where the LVC is usually confined within 200 AU from the star. In H2 2.122~μm, the LVC and HVC are spatially separated, with an abrupt transition from low- to high-velocity emission at ~1.5 arcsec. We moreover detect, for the first time, the fainter red-shifted counterpart down to the central source. This lobe shows several emission knots displaced symmetrically with respect to the corresponding blue-shifted gas. In HH1, we trace the jet down to ~1 arcsec from the VLA1 driving source: the kinematics of this inner region is again characterised by the presence of two velocity components, one blue-shifted and one red-shifted with respect to the source LSR velocity. We interpret this double component as arising from the interaction of two different jets. We suggest that the red-shifted component could be part of the HH501 jet. Electron densities and mass fluxes have been measured separately for the different velocity components in the HH34 and HH1 jets. In the inner HH34 jet region, ne increases with decreasing velocity, with an average value of ~1 × 104 cm-3 in the HVC and ~2.2 × 104 cm-3 in the LVC. Up to ~10 arcsec from the driving source, and along the whole HH1 jet an opposite behaviour is

  13. Jets from young stars

    NASA Astrophysics Data System (ADS)

    Bally, John

    2007-10-01

    Most stars produce spectacular jets during their formation. There are thousands of young stars within 500 pc of the Sun and many power jets. Thus protostellar jets may be the most common type of collimated astrophysical outflow. Shocks powered by outflows excite many emission lines, exhibit a rich variety of structure, and motions with velocities ranging from 50 to over 500 km s-1. Due to their relative proximity, proper motions and structural changes can be observed in less than a year. I review the general properties of protostellar jets, summarize some results from recent narrow-band imaging surveys of entire clouds, discuss irradiated jets, and end with some comments concerning outflows from high-mass young stellar objects. Protostellar outflows are ideal laboratories for the exploration of the jet physics.

  14. Relativistic Jets in Collapsars

    NASA Astrophysics Data System (ADS)

    Zhang, Weiqun; Woosley, S. E.; MacFadyen, A. I.

    2003-04-01

    We examine the propagation of two-dimensional relativistic jets through the stellar progenitor in the collapsar model for gamma-ray bursts. In agreement with previous studies, we find that the jet is collimated by its passage. Moreover, interaction of the jet with the star causes mixing that sporadically decelerates the jet, leading to a highly variable Lorentz factor. The jet that finally emerges has a moderate Lorentz factor, but a very large internal energy loading. In a second series of calculations we follow the emergence of such enegy-loaded jets from the star. For the initial conditions chosen, conversion of the remaining internal energy gives a terminal Lorentz factor of approximately 150. Implications of our calculations for GRB light curves, the luminosity-variability relation, and the GRB-supernova association are discussed.

  15. What ignites optical jets?

    SciTech Connect

    Sebastian Jester

    2002-12-23

    The properties of radio galaxies and quasars with and without optical or X-ray jets are compared. The majority of jets from which high-frequency emission has been detected so far (13 with optical emission, 11 with X-rays, 13 with both) are associated with the most powerful radio sources at any given redshift. It is found that optical/X-ray jet sources are more strongly beamed than the average population of extragalactic radio sources. This suggests that the detection or non-detection of optical emission from jets has so far been dominated by surface brightness selection effects, not by jet physics. It implies that optical jets are much more common than is currently appreciated.

  16. Visibility of Hinode/XRT X-Ray Jets at AIA/EUV Wavelengths, a Temperature Indicator

    NASA Astrophysics Data System (ADS)

    Sterling, A. C.; Bakucz Canario, D.; Moore, R. L.; Falconer, D. A.

    2015-12-01

    X-ray jets have been observed for years using data from the X-Ray Telescope (XRT) on the Hinode Satellite. Recently with the launch of the Solar Dynamics Observatory (SDO) it has been possible to observe solar jets over a range of EUV of wavelengths using the Atmospheric Imaging Assembly (AIA). In this study, we investigated the appearance of X-ray jets in AIA images at wavelengths of 304, 171, 193, 211, 131, 94, and 335 Å. We selected 20 random X-ray jets from XRT movies of the polar coronal holes and then examined AIA EUV images from the same locations and times to determine the visibility of the jets at the different EUV wavelengths. We found that the jets were almost always visible in the 193 and 211 Å channel images. In the "hottest" EUV channels (94 Å, 335 Å), usually the spire of the jet was not visible, although sometimes a base brightening could be discerned. At other wavelengths (171, 131, and 335), the results were mixed. Based on the response characteristics of AIA (Lemen et al, 2011) to the temperature of the observed radiating solar plasma, our finding that most jets are visible in the 193 and 211 Å channels is consistent with other recent studies that measured jet temperatures of 1.5~2.0 MK (Pucci et al, 2012 & Paraschiv et al, 2015). This work was supported by the NASA LWS and HGI programs.

  17. Phylogeny of Plant Calcium and Calmodulin-Dependent Protein Kinases (CCaMKs) and Functional Analyses of Tomato CCaMK in Disease Resistance.

    PubMed

    Wang, Ji-Peng; Munyampundu, Jean-Pierre; Xu, You-Ping; Cai, Xin-Zhong

    2015-01-01

    Calcium and calmodulin-dependent protein kinase (CCaMK) is a member of calcium/calmodulin-dependent protein kinase superfamily and is essential to microbe- plant symbiosis. To date, the distribution of CCaMK gene in plants has not yet been completely understood, and its function in plant disease resistance remains unclear. In this study, we systemically identified the CCaMK genes in genomes of 44 plant species in Phytozome and analyzed the function of tomato CCaMK (SlCCaMK) in resistance to various pathogens. CCaMKs in 18 additional plant species were identified, yet the absence of CCaMK gene in green algae and cruciferous species was confirmed. Sequence analysis of full-length CCaMK proteins from 44 plant species demonstrated that plant CCaMKs are highly conserved across all domains. Most of the important regulatory amino acids are conserved throughout all sequences, with the only notable exception being observed in N-terminal autophosphorylation site corresponding to Ser 9 in the Medicago truncatula CCaMK. CCaMK gene structures are similar, mostly containing six introns with a phase profile of 200200 and the exception was only noticed at the first exons. Phylogenetic analysis demonstrated that CCaMK lineage is likely to have diverged early from a calcium-dependent protein kinase (CDPK) gene in the ancestor of all nonvascular plant species. The SlCCaMK gene was widely and differently responsive to diverse pathogenic stimuli. Furthermore, knock-down of SlCCaMK reduced tomato resistance to Sclerotinia sclerotiorum and Pseudomonas syringae pv. tomato (Pst) DC3000 and decreased H2O2 accumulation in response to Pst DC3000 inoculation. Our results reveal that SlCCaMK positively regulates disease resistance in tomato via promoting H2O2 accumulation. SlCCaMK is the first CCaMK gene proved to function in plant disease resistance. PMID:26697034

  18. Phylogeny of Plant Calcium and Calmodulin-Dependent Protein Kinases (CCaMKs) and Functional Analyses of Tomato CCaMK in Disease Resistance

    PubMed Central

    Wang, Ji-Peng; Munyampundu, Jean-Pierre; Xu, You-Ping; Cai, Xin-Zhong

    2015-01-01

    Calcium and calmodulin-dependent protein kinase (CCaMK) is a member of calcium/calmodulin-dependent protein kinase superfamily and is essential to microbe- plant symbiosis. To date, the distribution of CCaMK gene in plants has not yet been completely understood, and its function in plant disease resistance remains unclear. In this study, we systemically identified the CCaMK genes in genomes of 44 plant species in Phytozome and analyzed the function of tomato CCaMK (SlCCaMK) in resistance to various pathogens. CCaMKs in 18 additional plant species were identified, yet the absence of CCaMK gene in green algae and cruciferous species was confirmed. Sequence analysis of full-length CCaMK proteins from 44 plant species demonstrated that plant CCaMKs are highly conserved across all domains. Most of the important regulatory amino acids are conserved throughout all sequences, with the only notable exception being observed in N-terminal autophosphorylation site corresponding to Ser 9 in the Medicago truncatula CCaMK. CCaMK gene structures are similar, mostly containing six introns with a phase profile of 200200 and the exception was only noticed at the first exons. Phylogenetic analysis demonstrated that CCaMK lineage is likely to have diverged early from a calcium-dependent protein kinase (CDPK) gene in the ancestor of all nonvascular plant species. The SlCCaMK gene was widely and differently responsive to diverse pathogenic stimuli. Furthermore, knock-down of SlCCaMK reduced tomato resistance to Sclerotinia sclerotiorum and Pseudomonas syringae pv. tomato (Pst) DC3000 and decreased H2O2 accumulation in response to Pst DC3000 inoculation. Our results reveal that SlCCaMK positively regulates disease resistance in tomato via promoting H2O2 accumulation. SlCCaMK is the first CCaMK gene proved to function in plant disease resistance. PMID:26697034

  19. Properties of gluon jets

    SciTech Connect

    Sugano, K.

    1986-09-01

    The properties of gluon jets are reviewed from an experimental point of view. The measured characteristics are compared to theoretical expectations. Although neither data nor models for the gluon jets are in the mature stage, there are remarkable agreements and also intriguing disagreements between experiment and theory. Since much interesting data have begun to emerge from various experiments and the properties of gluon jets are deeply rooted in the basic structure of non-Abelian gauge theory, the study of gluon jets casts further light on our understanding of QCD. Finally, the future prospects are discussed.

  20. Perspectives on jet noise

    NASA Technical Reports Server (NTRS)

    Ribner, H. S.

    1981-01-01

    Jet noise is a byproduct of turbulence. Until recently turbulence was assumed to be known statistically, and jet noise was computed therefrom. As a result of new findings though on the behavior of vortices and instability waves, a more integrated view of the problem has been accepted lately. After presenting a simple view of jet noise, the paper attempts to resolve the apparent differences between Lighthill's and Lilley's interpretations of mean-flow shear, and examines a number of ad hoc approaches to jet noise suppression.

  1. Interpretation of extragalactic jets

    SciTech Connect

    Norman, M.L.

    1985-01-01

    The nature of extragalatic radio jets is modeled. The basic hypothesis of these models is that extragalatic jets are outflows of matter which can be described within the framework of fluid dynamics and that the outflows are essentially continuous. The discussion is limited to the interpretation of large-scale (i.e., kiloparsec-scale) jets. The central problem is to infer the physical parameters of the jets from observed distributions of total and polarized intensity and angle of polarization as a function of frequency. 60 refs., 6 figs.

  2. Efficacy of MK615 for the treatment of patients with liver disorders

    PubMed Central

    Hokari, Atsushi; Ishikawa, Tomohisa; Tajiri, Hisao; Matsuda, Takahide; Ishii, Osamu; Matsumoto, Nobuyuki; Okuse, Chiaki; Takahashi, Hideaki; Kurihara, Takeshi; Kawahara, Ko-ichi; Maruyama, Ikuro; Zeniya, Mikio

    2012-01-01

    AIM: To investigate the hepatoprotective effect of MK615, a Japanese apricot extract, in an animal model, and its clinical therapeutic effect. METHODS: Wistar rats were administered physiological saline (4 mL/kg) or MK615 solution (4 mL/kg) for 7 d. On the sixth d, acute hepatic injury was induced by administering a single intraperitoneal injection (ip) of D-galactosamine hydrochloride (D-GalN) (600 mg/kg). Plasma levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined, and liver tissues were used for histopathological analysis. Fifty-eight patients with liver disorders [hepatitis C (n = 40), non-alcoholic fatty liver disease (n = 15), and autoimmune liver disease (n = 3)] were orally administered commercially available Misatol ME-containing MK615 (13 g/d) daily for 12 wk. Blood and urine were sampled immediately before and 6 wk, 12 wk, and 16 wk after the start of intake to measure various biochemical parameters. The percentage change in ALT and AST levels after 12 wk from the pre-intake baseline served as a primary endpoint. RESULTS: D-GalN effectively induced acute hepatic injury in the rats. At 48 h after the ip injection of D-GalN, the plasma levels of ALT (475.6 ± 191.5 IU/L vs 225.3 ± 194.2 IU/L, P < 0.05) and AST (1253.9 ± 223.4 IU/L vs 621.9 ± 478.2 IU/L, P < 0.05) in the MK615 group were significantly lower than the control group. Scattered single cell necrosis, loss of hepatocytes, and extensive inflammatory cell infiltration were observed in hepatic tissue samples collected from the control group. However, these findings were less pronounced in the group receiving MK615. At the end of the clinical study, serum ALT and AST levels were significantly decreased compared with pre-intake baseline levels from 103.5 ± 58.8 IU/L to 71.8 ± 39.3 IU/L (P < 0.05) and from 93.5 ± 55.6 IU/L to 65.5 ± 34.8 IU/L (P < 0.05), respectively. A reduction of ≥ 30% from the pre-study baseline ALT level was observed in 26 (45%) of

  3. Radiative versus Jet Mode in Radio Galaxies

    NASA Astrophysics Data System (ADS)

    Hardcastle, Martin

    2016-07-01

    In the local universe, the vast majority of radio-loud active galaxies show none of the conventional AGN apparatus of accretion disk, torus, corona, or broad/narrow-line regions. Instead such nuclear emission as they have appears to be completely dominated by emission directly from the jet; the accretion, which must be present to drive the jet, appears to be highly radiatively inefficient. However, the most radio-luminous objects in the universe are almost all quasars (type I or type II) which behave in the textbook manner, appearing as a normal radiatively efficient AGN with the addition of a jet. The past decade has seen a substantial evolution in our understanding of the physical origins of these differences, their relation to the host galaxy and environment, and their interpretation in terms of completely unified models of AGN, and I will review our current understanding of these issues in my talk.

  4. Effects of MK-801 upon local cerebral glucose utilization in conscious rats and in rats anaesthetised with halothane

    SciTech Connect

    Kurumaji, A.; McCulloch, J. )

    1989-12-01

    The effects of MK-801 (0.5 mg/kg i.v.), a non-competitive N-methyl-D-aspartate (NMDA) antagonist, upon local cerebral glucose utilization were examined in conscious, lightly restrained rats and in rats anaesthetised with halothane in nitrous oxide by means of the quantitative autoradiographic (14C)-2-deoxyglucose technique. In the conscious rats, MK-801 produced a heterogenous pattern of altered cerebral glucose utilization with significant increases being observed in 12 of the 28 regions of gray matter examined and significant decreases in 6 of the 28 regions. Pronounced increases in glucose use were observed after MK-801 in the olfactory areas and in a number of brain areas in the limbic system (e.g., hippocampus molecular layer, dentate gyrus, subicular complex, posterior cingulate cortex, and mammillary body). In the cerebral cortices, large reductions in glucose use were observed after administration of MK-801, whereas in the extrapyramidal and sensory-motor areas, glucose use remained unchanged after MK-801 administration in conscious rats. In the halothane-anaesthetised rats, the pattern of altered glucose use after MK-801 differed qualitatively and quantitatively from that observed in conscious rats. In anaesthetised rats, significant reductions in glucose use were noted after MK-801 in 10 of the 28 regions examined, with no area displaying significantly increased glucose use after administration of the drug. In halothane-anaesthetised rats, MK-801 failed to change the rates of glucose use in the olfactory areas, the hippocampus molecular layer, and the dentate gyrus.

  5. MK-4101, a Potent Inhibitor of the Hedgehog Pathway, Is Highly Active against Medulloblastoma and Basal Cell Carcinoma.

    PubMed

    Filocamo, Gessica; Brunetti, Mirko; Colaceci, Fabrizio; Sasso, Romina; Tanori, Mirella; Pasquali, Emanuela; Alfonsi, Romina; Mancuso, Mariateresa; Saran, Anna; Lahm, Armin; Di Marcotullio, Lucia; Steinkühler, Christian; Pazzaglia, Simonetta

    2016-06-01

    Aberrant activation of the Hedgehog (Hh) signaling pathway is implicated in the pathogenesis of many cancers, including medulloblastoma and basal cell carcinoma (BCC). In this study, using neonatally irradiated Ptch1(+/-) mice as a model of Hh-dependent tumors, we investigated the in vivo effects of MK-4101, a novel SMO antagonist, for the treatment of medulloblastoma and BCC. Results clearly demonstrated a robust antitumor activity of MK-4101, achieved through the inhibition of proliferation and induction of extensive apoptosis in tumor cells. Of note, beside antitumor activity on transplanted tumors, MK-4101 was highly efficacious against primary medulloblastoma and BCC developing in the cerebellum and skin of Ptch1(+/-) mice. By identifying the changes induced by MK-4101 in gene expression profiles in tumors, we also elucidated the mechanism of action of this novel, orally administrable compound. MK-4101 targets the Hh pathway in tumor cells, showing the maximum inhibitory effect on Gli1 MK-4101 also induced deregulation of cell cycle and block of DNA replication in tumors. Members of the IGF and Wnt signaling pathways were among the most highly deregulated genes by MK-4101, suggesting that the interplay among Hh, IGF, and Wnt is crucial in Hh-dependent tumorigenesis. Altogether, the results of this preclinical study support a therapeutic opportunity for MK-4101 in the treatment of Hh-driven cancers, also providing useful information for combination therapy with drugs targeting pathways cooperating with Hh oncogenic activity. Mol Cancer Ther; 15(6); 1177-89. ©2016 AACR. PMID:26960983

  6. MK571 inhibits phase-2 conjugation of flavonols by Caco-2/TC7 cells, but does not specifically inhibit their apical efflux☆

    PubMed Central

    Barrington, Robert D.; Needs, Paul W.; Williamson, Gary; Kroon, Paul A.

    2015-01-01

    MK571 is a multidrug resistance protein-2 (ABCC2, Mrp2) inhibitor and has been widely used to demonstrate the role of Mrp2 in the cellular efflux of drugs, xenobiotics and their conjugates. Numerous reports have described modulation of Caco-2 cellular efflux and transport of flavonoids in the presence of MK571. Since flavonoids are efficiently conjugated by Caco-2/TC7 cells, we investigated the effects of MK571 on the efflux of flavonoid conjugates. The flavonol aglycones kaempferol, quercetin and galangin were efficiently taken up, conjugated and effluxed by Caco-2/TC7 cells. Apically-applied MK571 caused significant reductions in both the apical and basolateral efflux of flavonol conjugates from Caco-2/TC7 monolayers. MK571 did not significantly alter the apical:basolateral efflux ratio for flavonol conjugates, however, which is not consistent with MK571 specifically inhibiting only apical Mrp2. Since MK571 decreased the total amounts of conjugates formed, and increased cellular flavonol aglycone concentrations, we explored the possibility that MK571 also inhibits phase-2 conjugation of flavonols. MK571 dose-dependently inhibited the intracellular biosynthesis of all flavonol glucuronides and sulphates by Caco-2 cells. MK571 significantly inhibited phase-2 conjugation of kaempferol by cell-free extracts of Caco-2, and production of kaempferol-4′-O-glucuronide was competitively inhibited. These data show that MK571, in addition to inhibiting MRP2, is a potential inhibitor of enterocyte phase-2 conjugation. PMID:25801004

  7. Enhancement of in vivo binding of [123I]beta-CIT by MK-801 in rat brain.

    PubMed

    Nakano, T; Takatoku, K; Matoba, Y; Iwamoto, B; Nishiura, M; Inoue, O; Nishimura, T

    1998-12-01

    The effects of MK-801, a noncompetitive NMDA receptor antagonist, on in vivo and in vitro binding of radioactive iodine ([123I] or [125I]) labeled beta-CIT [RTI-55, 3beta-(4-iodophenyl)tropane-2beta-carboxylic acid methyl ester] were investigated in rat brain. In the in vitro binding study, 10 pM of [125I]beta-CIT was incubated with either 0.03 microM or 3 microM of MK-801 at 24 degrees C for 60 min. In vitro, no alterations in [125I]beta-CIT binding in any region of rat brain slices were detected after addition of MK-801. In the in vivo binding study, [123I]beta-CIT was intravenously injected into rats 30 min after intraperitoneal injection of 0.03-1 mg/kg of MK-801. The in vivo [123I]beta-CIT binding in the striatum, frontal cortex, occipital cortex, hypothalamus, and thalamus was significantly increased by pretreatment with 1 mg/kg of MK-801. Kinetic analysis using the cerebellum as a reference region revealed that the increases in in vivo [123I]beta-CIT binding induced by MK-801 were mainly due to increases in both input rate constant k3 and output rate constant k4. The results of this study indicate that the glutamatergic system, including NMDA receptor, plays an important role in regulating neurotransmission in the dopaminergic or serotonergic systems in intact brain. PMID:9826232

  8. The CME Rate over Four Solar Cycles: Filling the Final Gap with MLSO MK3 Observations [1989-1996

    NASA Astrophysics Data System (ADS)

    St Cyr, O. C.; Flint, Q.; Quirk, C. A.; Burkepile, J.; Webb, D. F.; Lecinski, A. R.

    2013-12-01

    Coronal mass ejections (CMEs) were discovered in the early 1970's by the OSO-7 coronagraph, and large numbers were characterized for the first time by the Skylab ATM coronagraph. Since 1973 there has been only a single major gap in CME coverage in white light. Instruments that have contributed to estimates of the rate and properties of CMEs have included: Skylab ATM (1973-1974); Helios photometers (1974-1981); Solwind (1979-1985); SMM C/P (1980; 1984-1989); SOHO LASCO (1996-present); the Solar Mass Ejection Imager (SMEI, 2003-2011); and STEREO SECCHI (2006-present). We report here the first attempt to fill the 1989-1996 gap in the CME rate using the Mauna Loa Solar Observatory's MK3 K-coronameter. The MK3 instrument observed routinely several hours most days beginning in 1980 until it was upgraded to MK4 in 1998. MK3 CMEs detected from 1980-1989 were compared with Solwind and SMM and reported by St. Cyr et al. (1999). Since spaceborne instruments have more complete duty cycles than a groundbased instrument at a single location, we have 'calibrated' the MK3-derived CME rate from 1989 with the SMM C/P coronagraph, and from 1996 with the SOHO LASCO coronagraphs. CME rate calculations have been documented in Webb & Howard (1994), St. Cyr et al. (2000) and Robbrecht et al. (2009). Here we provide the preliminary CME rate calculation for 1989-1996 using the MLSO MK3 coronameter.

  9. FLARE-ASSOCIATED TYPE III RADIO BURSTS AND DYNAMICS OF THE EUV JET FROM SDO/AIA AND RHESSI OBSERVATIONS

    SciTech Connect

    Chen Naihwa; Ip, Wing-Huen; Innes, Davina E-mail: wingip@astro.ncu.edu.tw

    2013-06-01

    We present a detailed description of the interrelation between the Type III radio bursts and energetic phenomena associated with the flare activities in active region AR11158 at 07:58 UT on 2011 February 15. The timing of the Type III radio burst measured by the radio wave experiment on Wind/WAVE and an array of ground-based radio telescopes coincided with an extreme-ultraviolet (EUV) jet and hard X-ray (HXR) emission observed by SDO/AIA and RHESSI, respectively. There is clear evidence that the EUV jet shares the same source region as the HXR emission. The temperature of the jet, as determined by multiwavelength measurements by Atmospheric Imaging Assembly, suggests that Type III emission is associated with hot, 7 MK, plasma at the jet's footpoint.

  10. JetStar in flight

    NASA Technical Reports Server (NTRS)

    1981-01-01

    aircraft provided far-field acoustic data. Between May 21, 1981 and August of 1982, the JetStar completed roughly 45 research flights with three different propellers in varying configurations. Dryden engineers analyzed some of the resultant data, while they sent flight tapes to Hamilton Standard, Lewis, and Langley for analysis there. The results indicated a need for noise-reduction technology to keep the noise levels down to the project goals. An improved version of the advanced turboprop underwent flight testing in 1987 on a Gulfstream II over Georgia in 1987. These flight tests verified predictions of a 20- to 30-percent fuel savings. However, with the end of the energy crisis, the need for such savings disappeared, and the Advanced Turboprop Project did not lead to the expected industry-wide adoption of the new propeller systems on transport aircraft. In the 1960s, the same JetStar that was used to test the advanced turboprop had been equipped with an electronic variable-stability flight-control system. Called then a General Purpose Airborne Simulator (GPAS), the aircraft could duplicate the flight characteristics of a wide variety of advanced aircraft and was used for supersonic transport and general aviation research, and as a training and support system for Space Shuttle Approach and Landing Tests at Dryden in 1977. Over the years, the JetStar has also been used for a variety of other flight research projects, including laminar-flow-control flight tests in the mid-1980s.

  11. Comparative Analysis of Two Gene-Targeting Approaches Challenges the Tumor-Suppressive Role of the Protein Kinase MK5/PRAK

    PubMed Central

    Ronkina, Natalia; Johansen, Claus; Bohlmann, Lisa; Lafera, Juri; Menon, Manoj B.; Tiedje, Christopher; Laaß, Kathrin; Turk, Benjamin E.; Iversen, Lars; Kotlyarov, Alexey; Gaestel, Matthias

    2015-01-01

    MK5 (MAPK-activated protein kinase 5) or PRAK (p38-regulated and -activated kinase) are alternative names for a serine/threonine protein kinase downstream to ERK3/4 and p38 MAPK. A previous gene targeting approach for MK5/PRAK (termed here MK5/PRAK-Δex8) revealed a seemingly tumor-suppressive role of MK5/PRAK in DMBA-induced one step skin carcinogenesis and Ras-induced transformation. Here we demonstrate that an alternative targeting strategy of MK5/PRAK (termed MK5/PRAK-Δex6) increased neither tumor incidence in the one step skin carcinogenesis model, nor Ras-induced transformation in primary cells. Interestingly, due to the targeting strategies and exon skipping both knockouts do not completely abolish the generation of MK5/PRAK protein, but express MK5/PRAK deletion mutants with different biochemical properties depending on the exon targeted: Targeting of exon 6 leads to expression of an unstable cytoplasmic catalytically inactive MK5/PRAK-Δex6 mutant while targeting of exon 8 results in a more stable nuclear MK5/PRAK-Δex8 mutant with residual catalytic activity. The different properties of the MK5/PRAK deletion mutants could be responsible for the observed discrepancy between the knockout strains and challenge the role of MK5/PRAK in p53-dependent tumor suppression. Further MK5/PRAK knockout and knock-in mouse strains will be necessary to assign a physiological function to MK5/PRAK in this model organism. PMID:26295581

  12. Comparative Analysis of Two Gene-Targeting Approaches Challenges the Tumor-Suppressive Role of the Protein Kinase MK5/PRAK.

    PubMed

    Ronkina, Natalia; Johansen, Claus; Bohlmann, Lisa; Lafera, Juri; Menon, Manoj B; Tiedje, Christopher; Laaß, Kathrin; Turk, Benjamin E; Iversen, Lars; Kotlyarov, Alexey; Gaestel, Matthias

    2015-01-01

    MK5 (MAPK-activated protein kinase 5) or PRAK (p38-regulated and -activated kinase) are alternative names for a serine/threonine protein kinase downstream to ERK3/4 and p38 MAPK. A previous gene targeting approach for MK5/PRAK (termed here MK5/PRAK-Δex8) revealed a seemingly tumor-suppressive role of MK5/PRAK in DMBA-induced one step skin carcinogenesis and Ras-induced transformation. Here we demonstrate that an alternative targeting strategy of MK5/PRAK (termed MK5/PRAK-Δex6) increased neither tumor incidence in the one step skin carcinogenesis model, nor Ras-induced transformation in primary cells. Interestingly, due to the targeting strategies and exon skipping both knockouts do not completely abolish the generation of MK5/PRAK protein, but express MK5/PRAK deletion mutants with different biochemical properties depending on the exon targeted: Targeting of exon 6 leads to expression of an unstable cytoplasmic catalytically inactive MK5/PRAK-Δex6 mutant while targeting of exon 8 results in a more stable nuclear MK5/PRAK-Δex8 mutant with residual catalytic activity. The different properties of the MK5/PRAK deletion mutants could be responsible for the observed discrepancy between the knockout strains and challenge the role of MK5/PRAK in p53-dependent tumor suppression. Further MK5/PRAK knockout and knock-in mouse strains will be necessary to assign a physiological function to MK5/PRAK in this model organism. PMID:26295581

  13. Research on JET in view of ITER

    NASA Astrophysics Data System (ADS)

    Pamela, Jerome; Ongena, Jef; Watkins, Michael

    2004-11-01

    Research on JET is focused on further development of the two ITER reference plasma scenarios. The ELMy H-Mode, has been extended to lower rho* at high and q_95=3, with simultaneously H_98=0.9, and f_GW=0.9 at I_p=3.5 MA. The dependence of confinement on beta and rho* has been found to be more favorable than given by the IPB98(y,2) scaling. Highlights in the development of Advanced Regimes with Internal Transport Barriers (ITB) and strong reversed shear (q_0=2-3, q_min=1.5-2.5) are : (i) operation at a core density close to the Greenwald limit and (ii) full current drive in 3T/1.8MA ITB plasmas extended to 20 seconds with a JET record injected energy of E≈ 330MJ; (iii) 7 keV Te≈ Ti ITB plasmas at low toroidal rotation, and (iv) wide radius ITB's (r/a=0.6). Furthermore, emphasis in JET is placed on (i) mitigating the impact of ELMs, (ii) understanding the phenomena leading to tritium retention and (iii) preparing burning plasma physics. Recent developments on JET in view of ITER are : (i) real-time control in both ELMy H-Mode and ITB plasmas and (ii) an upgrade of JET with: (a) increased NBI power (b) a new ELM-resilient ITER-like ICRH antenna (7MW) to be tested in 2006 (c) 16 new and upgraded diagnostics.

  14. Aging jets from low-mass stars

    NASA Technical Reports Server (NTRS)

    Graham, J. A.; Chen, W. P.

    1994-01-01

    An extended faint optical jet is associated with the compact emission region plus faint star known as HH 55. HH 55 is located in the Lupus 2 cloud 2 min SW of the well studied T Tauri star RU Lupi. The HH 55 jet extends 55 sec N and 35 sec S in PA 160 deg. The HH 55 star is an emission line star of spectral type M3.5. Its image in the emission lines of H-alpha and (S II) is slightly elongated by 2 sec - 3 sec to the S but in continuum light is symmetrical and pointlike ((full width at half maximum) (FWHM) = 1.7 sec). The star and jet have several features in common with the star and jet known as Sz 102 = Th 28 in the nearby Lupus 3 cloud. We suggest that these objects are representative of the late evolutionary stage of the HH jet-outflow phenomenon and point out that such objects may be quite common although difficult to detect. With L(sub bol) approximately = 0.005 solar luminosity, and log T(sub e) approximately = 3.5, the HH 55 star is close to the main sequence and evolutionary tracks suggest an age of 3 x 10(exp 7) yr.

  15. Jet Lag in Athletes

    PubMed Central

    Lee, Aaron; Galvez, Juan Carlos

    2012-01-01

    Context: Prolonged transmeridian air travel can impart a physical and emotional burden on athletes in jet lag and travel fatigue. Jet lag may negatively affect the performance of athletes. Study Type: Descriptive review. Evidence Acquisition: A Medline search for articles relating to jet lag was performed (1990-present), as was a search relating to jet lag and athletes (1983-January, 2012). The results were reviewed for relevance. Eighty-nine sources were included in this descriptive review. Results: Behavioral strategies are recommended over pharmacological strategies when traveling with athletes; pharmacological aides may be used on an individual basis. Strategic sleeping, timed exposure to bright light, and the use of melatonin are encouraged. Conclusions: There is strong evidence that mood and cognition are adversely affected by jet lag. Some measures of individual and team performance are adversely affected as well. PMID:23016089

  16. Description of Jet Breakup

    NASA Technical Reports Server (NTRS)

    Papageorgiou, Demetrios T.

    1996-01-01

    In this article we review recent results on the breakup of cylindrical jets of a Newtonian fluid. Capillary forces provide the main driving mechanism and our interest is in the description of the flow as the jet pinches to form drops. The approach is to describe such topological singularities by constructing local (in time and space) similarity solutions from the governing equations. This is described for breakup according to the Euler, Stokes or Navier-Stokes equations. It is found that slender jet theories can be applied when viscosity is present, but for inviscid jets the local shape of the jet at breakup is most likely of a non-slender geometry. Systems of one-dimensional models of the governing equations are solved numerically in order to illustrate these differences.

  17. Multiple jet study

    NASA Technical Reports Server (NTRS)

    Walker, R. E.; Kors, D. L.

    1973-01-01

    Test data is presented which allows determination of jet penetration and mixing of multiple cold air jets into a ducted subsonic heated mainstream flow. Jet-to-mainstream momentum flux ratios ranged from 6 to 60. Temperature profile data is presented at various duct locations up to 24 orifice diameters downstream of the plane of jet injection. Except for two configurations, all geometries investigated had a single row of constant diameter orifices located transverse to the main flow direction. Orifice size and spacing between orifices were varied. Both of these were found to have a significant effect on jet penetration and mixing. The best mixing of the hot and cold streams was achieved with duct height.

  18. Jet physics at CDF

    SciTech Connect

    Melese, P.

    1997-05-01

    We present high E{sub T} jet measurements from CDF at the Fermilab Tevatron Collider. The incfilusive jet cross section at {radical}s = 1800 GeV with {approximately} 5 times more data is compared to the published CDF results, preliminary D0 results, and next-to-leading order QCD predictions. The {summation}E{sub T} cross section is also compared to QCD predictions and the dijet angular distribution is used to place a limit on quark compositeness. The inclusive jet cross section at {radical}s = 630 GeV is compared with that at 1800 GeV to test the QCD predictions for the scaling of jet cross sections with {radical}s. Finally, we present momentum distributions of charged particles in jets and compare them to Modified Leading Log Approximation predictions.

  19. Behavioral performance altering effects of MK-801 in zebrafish (Danio rerio)

    PubMed Central

    Sison, Margarette; Gerlai, Robert

    2011-01-01

    MK-801, a non-competitive NMDA-R antagonist, has been utilized in the analysis of mammalian learning and memory. The zebrafish is a novel vertebrate study species that has been proposed for the analysis of the mechanisms of learning and memory. Although learning paradigms have been developed for this species, psychopharmacological characterization of its behavioral responses is rudimentary. Before one attempts the analysis of the effects of MK-801 on learning and memory in zebrafish, one needs to know whether this drug affects motor function, perception and/or motivation, factors that may influence performance in learning tasks. Here we conduct dose response analyses investigating the effects of 0, 2, 20 and 100 µM MK-801 administered 24 hours or 30 minutes before the behavioral test, or during the test. We analyze responses in the open tank to measure motor and posture patterns, in the light dark paradigm to evaluate visual perception, and in a group preference task to attempt to quantify motivation. Our results show a significant performance alteration only in the highest (100 µM) dose groups. These fish spent more time on the bottom of their tank, showed elevated erratic movement, increased their clockwise and counterclockwise turning frequency, and reduced the time spent near a shoal stimulus, behavioral alterations that also depended upon the timing of drug administration. Thus, using the current delivery procedures and outbred zebrafish population, the highest dose that may not lead to significant performance deficits is 20 µM, a concentration we propose to use in a future learning study in zebrafish. PMID:21333690

  20. Pharmacological or genetic orexin1 receptor inhibition attenuates MK-801 induced glutamate release in mouse cortex.

    PubMed

    Aluisio, Leah; Fraser, Ian; Berdyyeva, Tamara; Tryputsen, Volha; Shireman, Brock T; Shoblock, James; Lovenberg, Timothy; Dugovic, Christine; Bonaventure, Pascal

    2014-01-01

    The orexin/hypocretin neuropeptides are produced by a cluster of neurons within the lateral posterior hypothalamus and participate in neuronal regulation by activating their receptors (OX1 and OX2 receptors). The orexin system projects widely through the brain and functions as an interface between multiple regulatory systems including wakefulness, energy balance, stress, reward, and emotion. Recent studies have demonstrated that orexins and glutamate interact at the synaptic level and that orexins facilitate glutamate actions. We tested the hypothesis that orexins modulate glutamate signaling via OX1 receptors by monitoring levels of glutamate in frontal cortex of freely moving mice using enzyme coated biosensors under inhibited OX1 receptor conditions. MK-801, an NMDA receptor antagonist, was administered subcutaneously (0.178 mg/kg) to indirectly disinhibit pyramidal neurons and therefore increase cortical glutamate release. In wild-type mice, pretreatment with the OX1 receptor antagonist GSK-1059865 (10 mg/kg S.C.) which had no effect by itself, significantly attenuated the cortical glutamate release elicited by MK-801. OX1 receptor knockout mice had a blunted glutamate release response to MK-801 and exhibited about half of the glutamate release observed in wild-type mice in agreement with the data obtained with transient blockade of OX1 receptors. These results indicate that pharmacological (transient) or genetic (permanent) inhibition of the OX1 receptor similarly interfere with glutamatergic function in the cortex. Selectively targeting the OX1 receptor with an antagonist may normalize hyperglutamatergic states and thus may represent a novel therapeutic strategy for the treatment of various psychiatric disorders associated with hyperactive states. PMID:24904253

  1. Pharmacological or genetic orexin1 receptor inhibition attenuates MK-801 induced glutamate release in mouse cortex

    PubMed Central

    Aluisio, Leah; Fraser, Ian; Berdyyeva, Tamara; Tryputsen, Volha; Shireman, Brock T.; Shoblock, James; Lovenberg, Timothy; Dugovic, Christine; Bonaventure, Pascal

    2014-01-01

    The orexin/hypocretin neuropeptides are produced by a cluster of neurons within the lateral posterior hypothalamus and participate in neuronal regulation by activating their receptors (OX1 and OX2 receptors). The orexin system projects widely through the brain and functions as an interface between multiple regulatory systems including wakefulness, energy balance, stress, reward, and emotion. Recent studies have demonstrated that orexins and glutamate interact at the synaptic level and that orexins facilitate glutamate actions. We tested the hypothesis that orexins modulate glutamate signaling via OX1 receptors by monitoring levels of glutamate in frontal cortex of freely moving mice using enzyme coated biosensors under inhibited OX1 receptor conditions. MK-801, an NMDA receptor antagonist, was administered subcutaneously (0.178 mg/kg) to indirectly disinhibit pyramidal neurons and therefore increase cortical glutamate release. In wild-type mice, pretreatment with the OX1 receptor antagonist GSK-1059865 (10 mg/kg S.C.) which had no effect by itself, significantly attenuated the cortical glutamate release elicited by MK-801. OX1 receptor knockout mice had a blunted glutamate release response to MK-801 and exhibited about half of the glutamate release observed in wild-type mice in agreement with the data obtained with transient blockade of OX1 receptors. These results indicate that pharmacological (transient) or genetic (permanent) inhibition of the OX1 receptor similarly interfere with glutamatergic function in the cortex. Selectively targeting the OX1 receptor with an antagonist may normalize hyperglutamatergic states and thus may represent a novel therapeutic strategy for the treatment of various psychiatric disorders associated with hyperactive states. PMID:24904253

  2. Neurobehavioral Differences Between Mice Receiving Distinct Neuregulin Variants as Neonates; Impact on Sensitivity to MK-801

    PubMed Central

    Kato, T.; Abe, Y.; Hirokawa, S.; Iwakura, Y.; Mizuno, M.; Namba, H.; Nawa, H.

    2015-01-01

    Neuregulin-1 (NRG1) is a well-recognized risk gene for schizophrenia and is often implicated in the neurodevelopmental hypothesis of this illness. Alternative splicing and proteolytic processing of the NRG1 gene produce more than 30 structural variants; however, the neuropathological roles of individual variants remain to be characterized. On the basis of the neurodevelopmental hypothesis of schizophrenia, we administered eNRG1 (0.1~1.0 µg/g), a core epidermal growth factor-like (EGF) domain common for all splicing NRG1 variants, to neonatal mice and compared their behavioral performance with mice challenged with a full mature form of type 1 NRG1 variant. During the neonatal stage, recombinant eNRG1 protein administrated from the periphery passed the blood-brain barrier and activated its receptor (ErbB4) in the brain. In adults, the mice receiving the highest dose exhibited lower locomotor activity and deficits in prepulse inhibition and tonedependent fear learning, although the hearing reduction of the eNRG1-treated mice may explain these behavioral deficits. Neonatal eNRG1 treatment also significantly potentiated MK-801-driven locomotor activity in an eNRG1 dose-dependent manner. In parallel eNRG1 treatment enhanced MK-801-driven c-Fos induction and decreased immunoreactivity for NMDA receptor subunits in adult brain. In contrast, mice that had been treated with the same molar dose of a full mature form of type 1 NRG1 as neonates did not exhibit hypersensitivity to MK-801. However, both animal models exhibited similar hypersensitivity to methamphetamine. Collectively, our findings suggest that aberrant peripheral NRG1 signals during neurodevelopment alter later behavioral traits and auditory functions in the NRG1 subtype-dependent manner. PMID:25817857

  3. Elastic measurements of TLSs in amorphous silicon at mK temperatures

    NASA Astrophysics Data System (ADS)

    Fefferman, Andrew; Liu, Xiao; Metcalf, Thomas; Jernigan, Glenn; Collin, Eddy

    The low temperature properties of glass are distinct from those of crystals due to the presence of poorly understood low-energy excitations. These are usually thought to be atoms tunneling between nearby equilibria, forming tunneling two level systems (TLSs). Elastic measurements on amorphous silicon films deposited with e-beam evaporation showed that this material contains a variable density of TLSs that decreases as the growth temperature increases from 45 to 400 deg C. We will present an analysis of the elastic properties of these films down to the low mK range in the framework of the standard tunneling model

  4. Conformational analysis of enalapril (MK-421) in solution by 1H and 13C NMR

    NASA Astrophysics Data System (ADS)

    Sakamoto, Yohko; Sakamoto, Yuko; Oonishi, Isao; Ohmoto, Taichi

    1990-10-01

    The conformations of enalapril (MK-421, (-)-( N-(( S)-1-ethoxycarbonyl-3-phenylpropyl)- L-alanyl- L-proline), in CD 3OD, were investigated in order to account for their biological activity as inhibitors of angiotensin converting enzyme (ACE). The ratio of trans to cis conformation around the amide bond is 3:2. The preferred optimum structures of the trans and cis forms are postulated. With reference to the proline ring the N-type trans isomer was more prevalent than the S-Type trans isomer.

  5. In vitro specular microscope perfusion of M-K- and moist chamber-stored human corneas.

    PubMed

    McCarey, B E

    1977-08-01

    Human donor corneas were stored in McCarey-Kaufman (M-K) medium for 3 to 6 days; then they were mounted in the specular microscope, and the endothelial surface perfused with a glutathione-bicarbonate-Ringer solution. During storage, the corneal thickness increased 37% above an assumed normal of 0.520 mm. The corneas did not have sufficient active thinning to be accepted as viable when tested by the temperature-reversal phenomenon. Since comparable donor corneas have been used in successful penetrating keratoplasties, there must be a discrepancy between the cell viability as tested by the temperature-reversal phenomenon and clinical application. PMID:328445

  6. Aryl or heteroaryl substituted aminal derivatives of HCV NS5A inhibitor MK-8742.

    PubMed

    Yu, Wensheng; Coburn, Craig A; Nair, Anilkumar G; Wong, Michael; Rosenblum, Stuart B; Zhou, Guowei; Dwyer, Michael P; Tong, Ling; Hu, Bin; Zhong, Bin; Hao, Jinglai; Ji, Tao; Zan, Shuai; Kim, Seong Heon; Zeng, Qingbei; Selyutin, Oleg; Chen, Lei; Masse, Frederic; Agrawal, Sony; Liu, Rong; Xia, Ellen; Zhai, Ying; Curry, Stephanie; McMonagle, Patricia; Ingravallo, Paul; Asante-Appiah, Ernest; Lin, Mingxiang; Kozlowski, Joseph A

    2016-08-01

    Herein we describe our research efforts around the aryl and heteroaryl substitutions at the aminal carbon of the tetracyclic indole-based HCV NS5A inhibitor MK-8742. A series of potent NS5A inhibitors are described, such as compounds 45-47, 54, 56, and 65, which showed improved potency against clinically relevant and resistance associated HCV variants. The improved potency profiles of these compounds demonstrated an SAR that can improve the potency against GT2b, GT1a Y93H, and GT1a L31V altogether, which was unprecedented in our previous efforts in NS5A inhibition. PMID:27394665

  7. Discovery of MK-5172, a Macrocyclic Hepatitis C Virus NS3/4a Protease Inhibitor

    PubMed Central

    2012-01-01

    A new class of HCV NS3/4a protease inhibitors containing a P2 to P4 macrocyclic constraint was designed using a molecular modeling-derived strategy. Building on the profile of previous clinical compounds and exploring the P2 and linker regions of the series allowed for optimization of broad genotype and mutant enzyme potency, cellular activity, and rat liver exposure following oral dosing. These studies led to the identification of clinical candidate 15 (MK-5172), which is active against genotype 1–3 NS3/4a and clinically relevant mutant enzymes and has good plasma exposure and excellent liver exposure in multiple species. PMID:24900473

  8. Performance of the BNL Mk-V steady state magnetron H source

    SciTech Connect

    Hershcovitch, A.; Prelec, K.; Alessi, J.

    1981-01-01

    The MkV magnetron source has been designed with the goal to achieve an H-or D-yield of 1-2 A in pulses longer than 5 a up to a steady state mode of operation. All electrodes are water cooled and this allows power densities up to 200 W/cm/sup 2/. The source has been operated in hydrogen and hydrogen-cesium modes. Various methods of cesium vapor injection have been tried and their reliability compared. The extractor grid in its first version has been made out of solid molybdenum, without cooling, and operated in a pulsed mode (pulse length more than 1 s).

  9. Computer analysis, design and construction of the BNL Mk V magnetron

    SciTech Connect

    McKenzie-Wilson, R.B.; Kovarik, V.J.

    1981-01-01

    As part of a program to develop a high energy neutral beam injector for fusion reactor applications, the BNL Neutral Beam Group is studying, among other options, a surface plasma source of the magnetron type. This source has been developed to the point at which a large compact model, known as the Mk V magnetron, has been designed and constructed. The source is designed to operate in the steady state mode and to produce 1-2A of H/sup -/(D/sup -/) ions at 25 kV. Under these conditions, 18 KW of heat are removed from the source by the cooling system.

  10. Cell type-specific pharmacology of NMDA receptors using masked MK801

    PubMed Central

    Yang, Yunlei; Lee, Peter; Sternson, Scott M

    2015-01-01

    N-Methyl-D-aspartate receptors (NMDA-Rs) are ion channels that are important for synaptic plasticity, which is involved in learning and drug addiction. We show enzymatic targeting of an NMDA-R antagonist, MK801, to a molecularly defined neuronal population with the cell-type-selectivity of genetic methods and the temporal control of pharmacology. We find that NMDA-Rs on dopamine neurons are necessary for cocaine-induced synaptic potentiation, demonstrating that cell type-specific pharmacology can be used to dissect signaling pathways within complex brain circuits. DOI: http://dx.doi.org/10.7554/eLife.10206.001 PMID:26359633

  11. Discovery of MK-3168: A PET Tracer for Imaging Brain Fatty Acid Amide Hydrolase.

    PubMed

    Liu, Ping; Hamill, Terence G; Chioda, Marc; Chobanian, Harry; Fung, Selena; Guo, Yan; Chang, Linda; Bakshi, Raman; Hong, Qingmei; Dellureficio, James; Lin, Linus S; Abbadie, Catherine; Alexander, Jessica; Jin, Hong; Mandala, Suzanne; Shiao, Lin-Lin; Li, Wenping; Sanabria, Sandra; Williams, David; Zeng, Zhizhen; Hajdu, Richard; Jochnowitz, Nina; Rosenbach, Mark; Karanam, Bindhu; Madeira, Maria; Salituro, Gino; Powell, Joyce; Xu, Ling; Terebetski, Jenna L; Leone, Joseph F; Miller, Patricia; Cook, Jacquelynn; Holahan, Marie; Joshi, Aniket; O'Malley, Stacey; Purcell, Mona; Posavec, Diane; Chen, Tsing-Bau; Riffel, Kerry; Williams, Mangay; Hargreaves, Richard; Sullivan, Kathleen A; Nargund, Ravi P; DeVita, Robert J

    2013-06-13

    We report herein the discovery of a fatty acid amide hydrolase (FAAH) positron emission tomography (PET) tracer. Starting from a pyrazole lead, medicinal chemistry efforts directed toward reducing lipophilicity led to the synthesis of a series of imidazole analogues. Compound 6 was chosen for further profiling due to its appropriate physical chemical properties and excellent FAAH inhibition potency across species. [(11)C]-6 (MK-3168) exhibited good brain uptake and FAAH-specific signal in rhesus monkeys and is a suitable PET tracer for imaging FAAH in the brain. PMID:24900701

  12. The Discovery of Quinoxaline-Based Metathesis Catalysts from Synthesis of Grazoprevir (MK-5172).

    PubMed

    Williams, Michael J; Kong, Jongrock; Chung, Cheol K; Brunskill, Andrew; Campeau, Louis-Charles; McLaughlin, Mark

    2016-05-01

    Olefin metathesis (OM) is a reliable and practical synthetic methodology for challenging carbon-carbon bond formations. While existing catalysts can effect many of these transformations, the synthesis and development of new catalysts is essential to increase the application breadth of OM and to achieve improved catalyst activity. The unexpected initial discovery of a novel olefin metathesis catalyst derived from synthetic efforts toward the HCV therapeutic agent grazoprevir (MK-5172) is described. This initial finding has evolved into a class of tunable, shelf-stable ruthenium OM catalysts that are easily prepared and exhibit unique catalytic activity. PMID:27123552

  13. The cool component and the dichotomy, lateral expansion, and axial rotation of solar X-ray jets

    SciTech Connect

    Moore, Ronald L.; Sterling, Alphonse C.; Falconer, David A.; Robe, Dominic

    2013-06-01

    We present results from a study of 54 polar X-ray jets that were observed in coronal X-ray movies from the X-ray Telescope on Hinode and had simultaneous coverage in movies of the cooler transition region (T ∼ 10{sup 5} K) taken in the He II 304 Å band of the Atmospheric Imaging Assembly (AIA) on Solar Dynamics Observatory. These dual observations verify the standard-jet/blowout-jet dichotomy of polar X-ray jets previously found primarily from XRT movies alone. In accord with models of blowout jets and standard jets, the AIA 304 Å movies show a cool (T ∼ 10{sup 5} K) component in nearly all blowout X-ray jets and in a small minority of standard X-ray jets, obvious lateral expansion in blowout X-ray jets but none in standard X-ray jets, and obvious axial rotation in both blowout X-ray jets and standard X-ray jets. In our sample, the number of turns of axial rotation in the cool-component standard X-ray jets is typical of that in the blowout X-ray jets, suggesting that the closed bipolar magnetic field in the jet base has substantial twist not only in all blowout X-ray jets but also in many standard X-ray jets. We point out that our results for the dichotomy, lateral expansion, and axial rotation of X-ray jets add credence to published speculation that type-II spicules are miniature analogs of X-ray jets, are generated by granule-size emerging bipoles, and thereby carry enough energy to power the corona and solar wind.

  14. Development test procedure High Pressure Water Jet System

    SciTech Connect

    Crystal, J.B.

    1995-06-05

    Development testing will be performed on the water jet cleaning fixture to determine the most effective arrangement of water jet nozzles to remove contamination from the surfaces of canisters and other debris. The following debris may be stained with dye to simulate surface contaminates: Mark O, Mark I, and Mark II Fuel Storage Canisters (both stainless steel and aluminum), pipe of various size, (steel, stainless, carbon steel and aluminum). Carbon steel and stainless steel plate, channel, angle, I-beam and other surfaces, specifically based on the Scientific Ecology Group (SEG) inventory and observations of debris within the basin. Test procedure for developmental testing of High Pressure Water Jet System.

  15. IS FS Tau B DRIVING AN ASYMMETRIC JET?

    SciTech Connect

    Liu, Chun-Fan; Shang, Hsien; Takami, Michihiro; Yan, Chi-Hung; Wang, Shiang-Yu; Ohashi, Nagayoshi; Pyo, Tae-Soo; Walter, Frederick M.; Hayashi, Masahiko

    2012-04-10

    FS Tau B is one of the few T Tauri stars that possess a jet and a counterjet as well as an optically visible cavity wall. We obtained images and spectra of its jet-cavity system in the near-infrared H and K bands using the Subaru/Infrared Camera and Spectrograph and detected the jet and the counterjet in the [Fe II] 1.644 {mu}m line for the first time. Within 2'' the blueshifted jet is brighter, whereas beyond {approx}5'' the redshifted counterjet dominates the [Fe II] emission. The innermost blueshifted knot is spectrally resolved to have a large line width of {approx}110 km s{sup -1}, while the innermost redshifted knot appears spectrally unresolved. The velocity ratio of the jet to the counterjet is {approx}1.34, which suggests that FS Tau B is driving an asymmetric jet, similar to those found in several T Tauri stars. Combining with optical observations in the literature, we showed that the blueshifted jet has a lower density and higher excitation than the redshifted counterjet. We suggest that the asymmetry in brightness and velocity is the manifestation of a bipolar outflow driving at different mass-loss rates, while maintaining balance of linear momentum. A full explanation of the asymmetry in the FS Tau B system awaits detail modeling and further investigation of the kinematic structure of the wind-associated cavity walls.

  16. Subchronic Exposure to Arsenic Represses the TH/TRβ1-CaMK IV Signaling Pathway in Mouse Cerebellum.

    PubMed

    Guan, Huai; Li, Shuangyue; Guo, Yanjie; Liu, Xiaofeng; Yang, Yi; Guo, Jinqiu; Li, Sheng; Zhang, Cong; Shang, Lixin; Piao, Fengyuan

    2016-01-01

    We previously reported that arsenic (As) impaired learning and memory by down-regulating calmodulin-dependent protein kinase IV (CaMK IV) in mouse cerebellum. It has been documented that the thyroid hormone receptor (TR)/retinoid X receptor (RXR) heterodimer and thyroid hormone (TH) may be involved in the regulation of CaMK IV. To investigate whether As affects the TR/RXR heterodimer and TH, we determined As concentration in serum and cerebellum, 3,5,3'-triiodothyronine (T3) and thyroxin (T4) levels in serum, and expression of CaMK IV, TR and RXR in cerebellum of mice exposed to As. Cognition function was examined by the step-down passive avoidance task and Morris water maze (MWM) tests. Morphology of the cerebellum was observed by Hematoxylin-Eosin staining under light microscope. Our results showed that the concentrations of As in the serum and cerebellum of mice both increased with increasing As-exposure level. A significant positive correlation was found between the two processes. Adeficit in learning and memory was found in the exposed mice. Abnormal morphologic changes of Purkinje cells were observed in cerebellum of the exposed mice. Moreover, the cerebellar expressions of CaMK IV protein and the TRβ gene, and TRβ1 protein were significantly lower in As-exposed mice than those in controls. Subchronic exposure to As appears to increase its level in serum and cerebella of mice, impairing learning and memory and down-regulating expression of TRβ1 as well as down-stream CaMK IV. It is also suggested that the increased As may be responsible for down-regulation of TRβ1 and CaMK IV in cerebellum and that the down-regulated TRβ1 may be involved in As-induced impairment of learning and memory via inhibiting CaMK IV and its down-stream pathway. PMID:26821021

  17. Wee1 inhibition by MK-1775 leads to tumor inhibition and enhances efficacy of gemcitabine in human sarcomas.

    PubMed

    Kreahling, Jenny M; Foroutan, Parastou; Reed, Damon; Martinez, Gary; Razabdouski, Tiffany; Bui, Marilyn M; Raghavan, Meera; Letson, Douglas; Gillies, Robert J; Altiok, Soner

    2013-01-01

    Sarcomas are rare and heterogeneous mesenchymal tumors affecting both pediatric and adult populations with more than 70 recognized histologies. Doxorubicin and ifosfamide have been the main course of therapy for treatment of sarcomas; however, the response rate to these therapies is about 10-20% in metastatic setting. Toxicity with the drug combination is high, response rates remain low, and improvement in overall survival, especially in the metastatic disease, remains negligible and new agents are needed. Wee1 is a critical component of the G2/M cell cycle checkpoint control and mediates cell cycle arrest by regulating the phosphorylation of CDC2. Inhibition of Wee1 by MK1775 has been reported to enhance the cytotoxic effect of DNA damaging agents in different types of carcinomas. In this study we investigated the therapeutic efficacy of MK1775 in various sarcoma cell lines, patient-derived tumor explants ex vivo and in vivo both alone and in combination with gemcitabine, which is frequently used in the treatment of sarcomas. Our data demonstrate that MK1775 treatment as a single agent at clinically relevant concentrations leads to unscheduled entry into mitosis and initiation of apoptotic cell death in all sarcomas tested. Additionally, MK1775 significantly enhances the cytotoxic effect of gemcitabine in sarcoma cells lines with different p53 mutational status. In patient-derived bone and soft tissue sarcoma samples we showed that MK1775 alone and in combination with gemcitabine causes significant apoptotic cell death. Magnetic resonance imaging (MRI) and histopathologic studies showed that MK1775 induces significant cell death and terminal differentiation in a patient-derived xenograft mouse model of osteosarcoma in vivo. Our results together with the high safety profile of MK1775 strongly suggest that this drug can be used as a potential therapeutic agent in the treatment of both adult as well as pediatric sarcoma patients. PMID:23520471

  18. Wee1 Inhibition by MK-1775 Leads to Tumor Inhibition and Enhances Efficacy of Gemcitabine in Human Sarcomas

    PubMed Central

    Kreahling, Jenny M.; Foroutan, Parastou; Reed, Damon; Martinez, Gary; Razabdouski, Tiffany; Bui, Marilyn M.; Raghavan, Meera; Letson, Douglas; Gillies, Robert J.; Altiok, Soner

    2013-01-01

    Sarcomas are rare and heterogeneous mesenchymal tumors affecting both pediatric and adult populations with more than 70 recognized histologies. Doxorubicin and ifosfamide have been the main course of therapy for treatment of sarcomas; however, the response rate to these therapies is about 10–20% in metastatic setting. Toxicity with the drug combination is high, response rates remain low, and improvement in overall survival, especially in the metastatic disease, remains negligible and new agents are needed. Wee1 is a critical component of the G2/M cell cycle checkpoint control and mediates cell cycle arrest by regulating the phosphorylation of CDC2. Inhibition of Wee1 by MK1775 has been reported to enhance the cytotoxic effect of DNA damaging agents in different types of carcinomas. In this study we investigated the therapeutic efficacy of MK1775 in various sarcoma cell lines, patient-derived tumor explants ex vivo and in vivo both alone and in combination with gemcitabine, which is frequently used in the treatment of sarcomas. Our data demonstrate that MK1775 treatment as a single agent at clinically relevant concentrations leads to unscheduled entry into mitosis and initiation of apoptotic cell death in all sarcomas tested. Additionally, MK1775 significantly enhances the cytotoxic effect of gemcitabine in sarcoma cells lines with different p53 mutational status. In patient-derived bone and soft tissue sarcoma samples we showed that MK1775 alone and in combination with gemcitabine causes significant apoptotic cell death. Magnetic resonance imaging (MRI) and histopathologic studies showed that MK1775 induces significant cell death and terminal differentiation in a patient-derived xenograft mouse model of osteosarcoma in vivo. Our results together with the high safety profile of MK1775 strongly suggest that this drug can be used as a potential therapeutic agent in the treatment of both adult as well as pediatric sarcoma patients. PMID:23520471

  19. Subchronic Exposure to Arsenic Represses the TH/TRβ1-CaMK IV Signaling Pathway in Mouse Cerebellum

    PubMed Central

    Guan, Huai; Li, Shuangyue; Guo, Yanjie; Liu, Xiaofeng; Yang, Yi; Guo, Jinqiu; Li, Sheng; Zhang, Cong; Shang, Lixin; Piao, Fengyuan

    2016-01-01

    We previously reported that arsenic (As) impaired learning and memory by down-regulating calmodulin-dependent protein kinase IV (CaMK IV) in mouse cerebellum. It has been documented that the thyroid hormone receptor (TR)/retinoid X receptor (RXR) heterodimer and thyroid hormone (TH) may be involved in the regulation of CaMK IV. To investigate whether As affects the TR/RXR heterodimer and TH, we determined As concentration in serum and cerebellum, 3,5,3’-triiodothyronine (T3) and thyroxin (T4) levels in serum, and expression of CaMK IV, TR and RXR in cerebellum of mice exposed to As. Cognition function was examined by the step-down passive avoidance task and Morris water maze (MWM) tests. Morphology of the cerebellum was observed by Hematoxylin-Eosin staining under light microscope. Our results showed that the concentrations of As in the serum and cerebellum of mice both increased with increasing As-exposure level. A significant positive correlation was found between the two processes. Adeficit in learning and memory was found in the exposed mice. Abnormal morphologic changes of Purkinje cells were observed in cerebellum of the exposed mice. Moreover, the cerebellar expressions of CaMK IV protein and the TRβ gene, and TRβ1 protein were significantly lower in As-exposed mice than those in controls. Subchronic exposure to As appears to increase its level in serum and cerebella of mice, impairing learning and memory and down-regulating expression of TRβ1 as well as down-stream CaMK IV. It is also suggested that the increased As may be responsible for down-regulation of TRβ1 and CaMK IV in cerebellum and that the down-regulated TRβ1 may be involved in As-induced impairment of learning and memory via inhibiting CaMK IV and its down-stream pathway. PMID:26821021

  20. Jet Noise Suppression

    NASA Technical Reports Server (NTRS)

    Gliebe, P. R.; Brausch, J. F.; Majjigi, R. K.; Lee, R.

    1991-01-01

    The objectives of this chapter are to review and summarize the jet noise suppression technology, to provide a physical and theoretical model to explain the measured jet noise suppression characteristics of different concepts, and to provide a set of guidelines for evolving jet noise suppression designs. The underlying principle for all jet noise suppression devices is to enhance rapid mixing (i.e., diffusion) of the jet plume by geometric and aerothermodynamic means. In the case of supersonic jets, the shock-cell broadband noise reduction is effectively accomplished by the elimination or mitigation of the shock-cell structure. So far, the diffusion concepts have predominantly concentrated on jet momentum and energy (kinetic and thermal) diffusion, in that order, and have yielded better noise reduction than the simple conical nozzles. A critical technology issue that needs resolution is the effect of flight on the noise suppression potential of mechanical suppressor nozzles. A more thorough investigation of this mechanism is necessary for the successful development and design of an acceptable noise suppression device for future high-speed civil transports.

  1. Angular Scaling In Jets

    SciTech Connect

    Jankowiak, Martin; Larkoski, Andrew J.; /SLAC

    2012-02-17

    We introduce a jet shape observable defined for an ensemble of jets in terms of two-particle angular correlations and a resolution parameter R. This quantity is infrared and collinear safe and can be interpreted as a scaling exponent for the angular distribution of mass inside the jet. For small R it is close to the value 2 as a consequence of the approximately scale invariant QCD dynamics. For large R it is sensitive to non-perturbative effects. We describe the use of this correlation function for tests of QCD, for studying underlying event and pile-up effects, and for tuning Monte Carlo event generators.

  2. The JET Neutron Activation System

    NASA Astrophysics Data System (ADS)

    Roquemore, A. L.; Bertalot, L.; Esposito, B.; Jarvis, O. N.; Loughlin, M. J.; Sadler, G.; van Belle, P.

    1997-11-01

    The JET activation system provides the absolute value of the neutron yields as well as a check on the linearity of other neutron detector systems. The total neutron yield is standardized to one irradiation end reentrant in the top of the vessel, while the results from the other seven irradiation ends are normalized to this standard end and provide redundancy as well as information on the plasma position. A pneumatic transfer system is used to transfer up to five capsules containing elemental foils for a single discharge on JET. Eleven different elemental foils have been utilized to determine the yields from both DD and DT plasmas. By placing several different foils with different activation energy thresholds in a single capsule for one DT discharge, neutron spectral information has been obtained by use of the SAND-II unfolding code. A description of the activation system hardware and calibration of the activation detector system will be presented along with the results from the DT neutron calibration campaign.

  3. A CCaMK-CYCLOPS-DELLA Complex Activates Transcription of RAM1 to Regulate Arbuscule Branching.

    PubMed

    Pimprikar, Priya; Carbonnel, Samy; Paries, Michael; Katzer, Katja; Klingl, Verena; Bohmer, Monica J; Karl, Leonhard; Floss, Daniela S; Harrison, Maria J; Parniske, Martin; Gutjahr, Caroline

    2016-04-25

    Intracellular arbuscular mycorrhiza symbiosis between plants and glomeromycotan fungi leads to formation of highly branched fungal arbuscules that release mineral nutrients to the plant host. Their development is regulated in plants by a mechanistically unresolved interplay between symbiosis, nutrient, and hormone (gibberellin) signaling. Using a positional cloning strategy and a retrotransposon insertion line, we identify two novel alleles of Lotus japonicus REDUCED ARBUSCULAR MYCORRHIZA1 (RAM1) encoding a GRAS protein. We confirm that RAM1 is a central regulator of arbuscule development: arbuscule branching is arrested in L. japonicus ram1 mutants, and ectopic expression of RAM1 activates genes critical for arbuscule development in the absence of fungal symbionts. Epistasis analysis places RAM1 downstream of CCaMK, CYCLOPS, and DELLA because ectopic expression of RAM1 restores arbuscule formation in cyclops mutants and in the presence of suppressive gibberellin. The corresponding proteins form a complex that activates RAM1 expression via binding of CYCLOPS to a cis element in the RAM1 promoter. We thus reveal a transcriptional cascade in arbuscule development that employs the promoter of RAM1 as integrator of symbiotic (transmitted via CCaMK and CYCLOPS) and hormonal (gibberellin) signals. PMID:27020747

  4. A scanning Hall probe microscope for high resolution magnetic imaging down to 300 mK

    NASA Astrophysics Data System (ADS)

    Khotkevych, V. V.; Milošević, M. V.; Bending, S. J.

    2008-12-01

    We present the design, construction, and performance of a low-temperature scanning Hall probe microscope with submicron lateral resolution and a large scanning range. The detachable microscope head is mounted on the cold flange of a commercial H3e-refrigerator (Oxford Instruments, Heliox VT-50) and operates between room temperature and 300 mK. It is fitted with a three-axis slip-stick nanopositioner that enables precise in situ adjustment of the probe location within a 6×6×7 mm3 space. The local magnetic induction at the sample surface is mapped with an easily changeable microfabricated Hall probe [typically GsAs/AlGaAs or AlGaAs/InGaAs/GaAs Hall sensors with integrated scanning tunnel microscopy (STM) tunneling tips] and can achieve minimum detectable fields ⩾10 mG/Hz1/2. The Hall probe is brought into very close proximity to the sample surface by sensing and controlling tunnel currents at the integrated STM tip. The instrument is capable of simultaneous tunneling and Hall signal acquisition in surface-tracking mode. We illustrate the potential of the system with images of superconducting vortices at the surface of a Nb thin film down to 372 mK, and also of labyrinth magnetic-domain patterns of an yttrium iron garnet film captured at room temperature.

  5. A scanning Hall probe microscope for high resolution magnetic imaging down to 300 mK

    SciTech Connect

    Khotkevych, V. V.; Bending, S. J.; Milosevic, M. V.

    2008-12-15

    We present the design, construction, and performance of a low-temperature scanning Hall probe microscope with submicron lateral resolution and a large scanning range. The detachable microscope head is mounted on the cold flange of a commercial {sup 3}He-refrigerator (Oxford Instruments, Heliox VT-50) and operates between room temperature and 300 mK. It is fitted with a three-axis slip-stick nanopositioner that enables precise in situ adjustment of the probe location within a 6x6x7 mm{sup 3} space. The local magnetic induction at the sample surface is mapped with an easily changeable microfabricated Hall probe [typically GsAs/AlGaAs or AlGaAs/InGaAs/GaAs Hall sensors with integrated scanning tunnel microscopy (STM) tunneling tips] and can achieve minimum detectable fields {>=}10 mG/Hz{sup 1/2}. The Hall probe is brought into very close proximity to the sample surface by sensing and controlling tunnel currents at the integrated STM tip. The instrument is capable of simultaneous tunneling and Hall signal acquisition in surface-tracking mode. We illustrate the potential of the system with images of superconducting vortices at the surface of a Nb thin film down to 372 mK, and also of labyrinth magnetic-domain patterns of an yttrium iron garnet film captured at room temperature.

  6. A CaMK cascade activates CRE-mediated transcription in neurons of Caenorhabditis elegans

    PubMed Central

    Kimura, Yoshishige; Corcoran, Ethan E.; Eto, Koh; Gengyo-Ando, Keiko; Muramatsu, Masa-aki; Kobayashi, Ryoji; Freedman, Jonathan H.; Mitani, Shohei; Hagiwara, Masatoshi; Means, Anthony R.; Tokumitsu, Hiroshi

    2002-01-01

    Calcium (Ca2+) signals regulate a diverse set of cellular responses, from proliferation to muscular contraction and neuro-endocrine secretion. The ubiquitous Ca2+ sensor, calmodulin (CaM), translates changes in local intracellular Ca2+ concentrations into changes in enzyme activities. Among its targets, the Ca2+/CaM-dependent protein kinases I and IV (CaMKs) are capable of transducing intraneuronal signals, and these kinases are implicated in neuronal gene regulation that mediates synaptic plasticity in mammals. Recently, the cyclic AMP response element binding protein (CREB) has been proposed as a target for a CaMK cascade involving not only CaMKI or CaMKIV, but also an upstream kinase kinase that is also CaM regulated (CaMKK). Here, we report that all components of this pathway are coexpressed in head neurons of Caenorhabditis elegans. Utilizing a transgenic approach to visualize CREB-dependent transcription in vivo, we show that this CaMK cascade regulates CRE-mediated transcription in a subset of head neurons in living nematodes. PMID:12231504

  7. Multiwavelength Observations of AGN Jets: Untangling the Coupled Problems of Emission Mechanism and Jet Structure

    NASA Astrophysics Data System (ADS)

    Perlman, Eric S.; Avachat, Sayali S.; Clautice, Devon; Georganopoulos, Markos; Meyer, Eileen; Cara, Mihai

    2016-04-01

    The discovery of X-ray and optical emission from large numbers of AGN jets is one of the key legacies of the Chandra X-ray Observatory and Hubble Space Telescope. Several dozen optical and X-ray emitting jets are now known, most of which are seen in both bands as well as in the radio, where they were first discovered. Jets carry prodigious amounts of energy and mass out from the nuclear regions out to tens to hundreds of kiloparsecs distant from the central black hole, depositing it into the host galaxy and cluster. Interpreting their multiwavelength emissions has not been easy: while in most jets, the optical and radio emission in many objects is believed to emerge via the synchrotron process, due to its characteristic spectral shape and high radio polarization, the X-ray emission has been a tougher nut to crack. In less powerful, FR I jets, such as M87, the X-ray emission is believed to be synchrotron emission from the highest energy electrons, requiring in situ particle acceleration due to the short radiative lifetimes of the particles. However, in FR II and quasar jets, a variety of emission mechanisms are possible. Until the last few years, the leading interpretation had been inverse-Comptonization of Cosmic Microwave Background photons (the IC/CMB mechanism). This requires the jet to be relativistic out to hundreds of kiloparsecs from the nucleus, and requires an electron spectrum that extends to very low Lorentz factors. However, that now appears less likely, due to observed high optical polarizations in jets where the optical and X-ray emission appears to lie on the same spectral component, as well as limits derived from Fermi observations in the GeV gamma-rays. It now appears more likely that the X-rays must arise as synchrotron emission from a second, high energy electron population. With this revelation, we must tackle anew the coupling between jet structure and emission mechanisms. Multiwavelength imaging and polarimetry can give us clues to the

  8. Prostaglandin D2 signaling mediated by the CRTH2 receptor is involved in MK-801-induced cognitive dysfunction.

    PubMed

    Onaka, Yusuke; Shintani, Norihito; Nakazawa, Takanobu; Kanoh, Takuya; Ago, Yukio; Matsuda, Toshio; Hashimoto, Ryota; Ohi, Kazutaka; Hirai, Hiroyuki; Nagata, Kin-Ya; Nakamura, Masataka; Kasai, Atsushi; Hayata-Takano, Atsuko; Nagayasu, Kazuki; Takuma, Kazuhiro; Ogawa, Asao; Baba, Akemichi; Hashimoto, Hitoshi

    2016-11-01

    Chemoattractant receptor-homologous molecule expressed on T helper type 2 cells (CRTH2), which is a second receptor for prostaglandin (PG) D2, is involved in inflammatory responses in peripheral tissue; however, its role in cognitive function remains unclear. Here, we demonstrate that CRTH2 is involved in cognitive function using a well-established animal model of cognitive dysfunction induced by MK-801, an N-methyl-d-aspartate receptor antagonist. Genetic deletion and pharmacological inhibition of CRTH2 suppressed MK-801-induced cognitive dysfunction. Pharmacological inhibition of cyclooxygenase-1, a rate-limiting enzyme in PG synthesis, also suppressed MK-801-induced cognitive dysfunction. Moreover, an MK-801-induced increase in c-Fos expression in the paraventricular nucleus (PVN) was abolished in the CRTH2-deficient mice. Together, these results suggest that PGD2-CRTH2 signaling is involved in both MK-801-induced cognitive dysfunction and neuronal activity regulation in the PVN. Furthermore, genetic association studies suggest that CRTH2 is weakly associated with cognitive function in humans. Our study provides evidence that PGD2-CRTH2 signaling is involved in cognitive function and may represent a potential therapeutic target for cognitive dysfunction in patients with psychiatric disorders. PMID:27481693

  9. Impairment of the Anterior Thalamic Head Direction Cell Network Following Administration of the NMDA antagonist MK-801

    PubMed Central

    Housh, Adam A.; Berkowitz, Laura E.; Ybarra, Isaac; Kim, Esther U.; Lee, Brian R.; Calton, Jeffrey L.

    2014-01-01

    Head direction (HD) cells, found in the rodent Papez circuit, are thought to form the neural circuitry responsible for directional orientation. Because NMDA transmission has been implicated in spatial tasks requiring directional orientation, we sought to determine if the NMDA antagonist dizocilpine (MK-801) would disrupt the directional signal carried by the HD network. Anterior thalamic HD cells were isolated in female Long-Evans rats and initially monitored for baseline directional activity while the animals foraged in a familiar enclosure. The animals were then administered MK-801 at a dose of .05 mg/kg or 0.1 mg/kg, or isotonic saline, and cells were re-examined for changes in directional specificity and landmark control. While the cells showed no changes in directional specificity and landmark control following administration of saline or the lower dose of MK-801, the higher dose of MK-801 caused a dramatic attenuation of the directional signal, characterized by decreases in peak firing rates, signal to noise, and directional information content. While the greatly attenuated directional specificity of cells in the high dose condition usually remained stable relative to the landmarks within the recording enclosure, a few cells in this condition exhibited unstable preferred directions within and between recording sessions. Our results are discussed relative to the possibility that the findings explain the effects of MK-801 on the acquisition and performance of spatial tasks. PMID:25307435

  10. Qualification campaign of the 50 mK hybrid sorption-ADR cooler for SPICA/SAFARI

    NASA Astrophysics Data System (ADS)

    Duval, J.-M.; Duband, L.; Attard, A.

    2015-12-01

    SAFARI (SpicA FAR-infrared Instrument) is an infrared instrument planned to be part of the SPICA (SPace Infrared telescope for Cosmology and Astrophysics) Satellite. It will offer high spectral resolution in the 30 - 210 μm frequency range. SAFARI will benefit from the cold telescope of SPICA and to obtain the required detectors sensitivity, a temperature of 50 mK is required. This temperature is reached thanks to the use of a hybrid sorption - ADR (Adiabatic Demagnetization Refrigerator) cooler presented here. This cooler provides respectively 14 μW and 0.4 μW of cooling power at 300 mK and 50 mK. The cooler is planned to advantageously use two thermal interfaces of the instrument at 1.8 and 4.9 K. One of the challenges discussed in this paper is the low power available at each intercept. A dedicated laboratory electronic is being designed based on previous development with a particular focus on the 50 mK readout. Temperature regulation at 50 mK is also discussed. This cooler has been designed following flight constraints and will reach a high TRL, including mechanical and environmental tests at the end of the on-going qualification campaign.

  11. Dizocilpine (MK-801) arrests status epilepticus and prevents brain damage induced by Soman. (Reannouncement with new availability information)

    SciTech Connect

    Sparenborg, S.; Brennecke, L.H.; Jaax, N.K.; Braitman, D.J.

    1992-12-31

    The involvement of the NMDA receptor in the neurotoxicity induced by soman, an organophosphorus compound which irreversibly inhibits cholinesterase, was studied in guinea pigs. The drug MK-801 (0.5, 1 or 5 mg/kg, i.p.) was given as a pretreatment before a convulsant dose of soman or as a post treatment (30, 100 or 300 micron g/kg, i.m.) 5 min after the development of soman-induced status epilepticus. Pyridostigmine, atropine and pralidoxime chloride were also given to each subject to counteract the lethality of soman. All subjects that were challenged with soman and given the vehicle for MK-801 (saline) exhibited severe convulsions and electrographic seizure activity. Neuronal necrosis was found in the hippocampus, amygdala, thalamus and the pyriform and cerebral cortices of those subjects surviving for 48 hr. Pretreatment with 0.5 or 1 mg/kg doses of MK-801 did not prevent nor delay the onset of seizure activity but did diminish its intensity and led to its early arrest. At the largest dose (5 mg/kg), MK-801 completely prevented the development of seizure activity and brain damage. Post treatment with MK-801 prevented, arrested or reduced seizure activity, convulsions and neuronal necrosis in a dose-dependent manner. The NMDA receptor may play a more critical role in the spread and maintenance, rather than the initiation of cholinergically-induced seizure activity....Seizure-related brain damage, Organophosphorus compound, Nerve agent, Cholinesterase inhibition, Excitotoxicity, Guinea pig.

  12. The FvMK1 mitogen-activated protein kinase gene regulates conidiation, pathogenesis, and fumonisin production in Fusarium verticillioides.

    PubMed

    Zhang, Yueping; Choi, Yoon-E; Zou, Xuexiao; Xu, Jin-Rong

    2011-02-01

    Fusarium verticillioides is one of the most important fungal pathogens to cause destructive diseases of maize worldwide. Fumonisins produced by the fungus are harmful to human and animal health. To date, our understanding of the molecular mechanisms associated with pathogenicity and fumonisin biosynthesis in F. verticillioides is limited. Because MAP kinase pathways have been implicated in regulating diverse processes important for plant infection in phytopathogenic fungi, in this study we identified and functionally characterized the FvMK1 gene in F. verticillioides. FvMK1 is orthologous to FMK1 in F. oxysporum and GPMK1 in F. graminearum. The Fvmk1 deletion mutant was reduced in vegetative growth and production of microconidia. However, it was normal in sexual reproduction and increased in the production of macroconidia. In infection assays with developing corn kernels, the Fvmk1 mutant was non-pathogenic and failed to colonize through wounding sites. It also failed to cause stalk rot symptoms beyond the inoculation sites on corn stalks, indicating that FvMK1 is essential for plant infection. Furthermore, the Fvmk1 mutant was significantly reduced in fumonisin production and expression levels of FUM1 and FUM8, two genes involved in fumonisin biosynthesis. The defects of the Fvmk1 mutant were fully complemented by re-introducing the wild type FvMK1 allele. These results demonstrate that FvMK1 plays critical roles in the regulation of vegetative growth, asexual reproduction, fumonisin biosynthesis, and pathogenicity. PMID:20887797

  13. Dilution jet mixing program

    NASA Technical Reports Server (NTRS)

    Srinivasan, R.; Coleman, E.; Johnson, K.

    1984-01-01

    Parametric tests were conducted to quantify the mixing of opposed rows of jets (two-sided injection) in a confined cross flow. Results show that jet penetrations for two sided injections are less than that for single-sided injections, but the jet spreading rates are faster for a given momentum ratio and orifice plate. Flow area convergence generally enhances mixing. Mixing characteristics with asymmetric and symmetric convergence are similar. For constant momentum ratio, the optimum S/H(0) with in-line injections is one half the optimum value for single sided injections. For staggered injections, the optimum S/H(0) is twice the optimum value for single-sided injection. The correlations developed predicted the temperature distributions within first order accuracy and provide a useful tool for predicting jet trajectory and temperature profiles in the dilution zone with two-sided injections.

  14. Jet lag prevention

    MedlinePlus

    ... your internal clock before you travel. While in flight: DO NOT sleep unless it matches the bedtime ... decrease jet lag. If you will be in flight during the bedtime of your destination, take some ...

  15. Counterflowing Jet Subsystem Design

    NASA Technical Reports Server (NTRS)

    Farr, Rebecca; Daso, Endwell; Pritchett, Victor; Wang, Ten-See

    2010-01-01

    A counterflowing jet design (a spacecraft and trans-atmospheric subsystem) employs centrally located, supersonic cold gas jets on the face of the vehicle, ejecting into the oncoming free stream. Depending on the supersonic free-stream conditions and the ejected mass flow rate of the counterflowing jets, the bow shock of the vehicle is moved upstream, further away from the vehicle. This results in an increasing shock standoff distance of the bow shock with a progressively weaker shock. At a critical jet mass flow rate, the bow shock becomes so weak that it is transformed into a series of compression waves spread out in a much wider region, thus significantly modifying the flow that wets the outer surfaces, with an attendant reduction in wave and skin friction drag and aerothermal loads.

  16. P2-quinazolinones and bis-macrocycles as new templates for next-generation hepatitis C virus NS3/4a protease inhibitors: discovery of MK-2748 and MK-6325.

    PubMed

    Rudd, Michael T; Butcher, John W; Nguyen, Kevin T; McIntyre, Charles J; Romano, Joseph J; Gilbert, Kevin F; Bush, Kimberly J; Liverton, Nigel J; Holloway, M Katharine; Harper, Steven; Ferrara, Marco; DiFilippo, Marcello; Summa, Vincenzo; Swestock, John; Fritzen, Jeff; Carroll, Steven S; Burlein, Christine; DiMuzio, Jillian M; Gates, Adam; Graham, Donald J; Huang, Qian; McClain, Stephanie; McHale, Carolyn; Stahlhut, Mark W; Black, Stuart; Chase, Robert; Soriano, Aileen; Fandozzi, Christine M; Taylor, Anne; Trainor, Nicole; Olsen, David B; Coleman, Paul J; Ludmerer, Steven W; McCauley, John A

    2015-04-01

    With the goal of identifying inhibitors of hepatitis C virus (HCV) NS3/4a protease that are potent against a wide range of genotypes and clinically relevant mutant viruses, several subseries of macrocycles were investigated based on observations made during the discovery of MK-5172. Quinazolinone-containing macrocycles were identified as promising leads, and optimization for superior cross-genotype and mutant enzyme potency as well as rat liver and plasma concentrations following oral dosing, led to the development of MK-2748. Additional investigation of a series of bis-macrocycles containing a fused 18- and 15-membered ring system were also optimized for the same properties, leading to the discovery of MK-6325. Both compounds display the broad genotype and mutant potency necessary for clinical development as next-generation HCV NS3/4a protease inhibitors. PMID:25759009

  17. Jets in hadronic reactions

    SciTech Connect

    Paige, F.E.

    1983-01-01

    Recent experimental data on the properties of jets in hadronic reactions are reviewed and compared with theoretical expectations. Jets are clearly established as the dominant process for high E/sub T/ events in hadronic reactions. The cross section and the other properties of these events are in qualitative and even semiquantitative agreement with expectations based on perturbative QCD. However, we can not yet make precise tests of QCD, primarily because there are substantial uncertainties in the theoretical calculations. 45 references. (WHK)

  18. Radiation from Relativistic Jets

    NASA Technical Reports Server (NTRS)

    Nishikawa, K.-I.; Mizuno, Y.; Hardee, P.; Sol, H.; Medvedev, M.; Zhang, B.; Nordlund, A.; Frederiksen, J. T.; Fishman, G. J.; Preece, R.

    2008-01-01

    Nonthermal radiation observed from astrophysical systems containing relativistic jets and shocks, e.g., gamma-ray bursts (GRBs), active galactic nuclei (AGNs), and Galactic microquasar systems usually have power-law emission spectra. Recent PIC simulations of relativistic electron-ion (electron-positron) jets injected into a stationary medium show that particle acceleration occurs within the downstream jet. In the presence of relativistic jets, instabilities such as the Buneman instability, other two-streaming instability, and the Weibel (filamentation) instability create collisionless shocks, which are responsible for particle (electron, positron, and ion) acceleration. The simulation results show that the Weibel instability is responsible for generating and amplifying highly nonuniform, small-scale magnetic fields. These magnetic fields contribute to the electron's transverse deflection behind the jet head. The 'jitter' radiation from deflected electrons in small-scale magnetic fields has different properties than synchrotron radiation which is calculated in a uniform magnetic field. This jitter radiation, a case of diffusive synchrotron radiation, may be important to understand the complex time evolution and/or spectral structure in gamma-ray bursts, relativistic jets, and supernova remnants.

  19. On the stability and energy dissipation in magnetized radio galaxy jets.

    NASA Astrophysics Data System (ADS)

    Bromberg, Omer; Tchekhovskoy, Alexander

    2016-07-01

    It is commonly accepted that the relativistic jets observed in radio galaxies are launched magnetically and are powered by the rotational energy of the central supermassive black hole. Such jets carry most of their energy in the form of electromagnetic Poynting flux. However by the time the ejecta reach the emission zone most of that energy is transferred to relativistic motions of the jet material with a large fraction given to non-thermal particles, which calls for an efficient dissipation mechanism to work within the jet without compromising its integrity. Understanding the energy dissipation mechanisms and stability of Poynting flux dominated jets is therefore crucial for modeling these astrophysical objects. In this talk I will present the first self consistent 3D simulations of the formation and propagation of highly magnetized (σ ˜25), relativistic jets in a medium. We find that the jets develop two types of instability: i) a local, "internal" kink mode which efficiently dissipates half of the magnetic energy into heat, and ii) a global "external" mode that grows on longer time scales and causes the jets to bend sideways and wobble. Low power jets propagating in media with flat density profiles, such as galaxy cluster cores, are susceptible to the global mode, and develop FRI like morphology. High power jets remain stable as they cross the cores, break out and accelerate to large distances, appearing as FRII jets. Thus magnetic kink instability can account for both the magnetic energy dissipation and the population dichotomy in radio galaxy jets.

  20. W/Z+ jets and Z p_t measurements at Tevatron

    SciTech Connect

    Chung, Y.S.; /Rochester U.

    2006-10-01

    The authors present a measurement of W/Z boson + jets production and Z p{sub T} measurement in the p{bar p} collisions at the Tevatron Collider at {radical}s = 1.96 TeV. The CDF II measures W + jets production based on 320 pb{sup -1} and the D0 Run II measures Z + jets with 950 pb{sup -1} data. The measurement of Z p{sub T} is performed with D0 Run II data corresponding to an integrated luminosity of 960 pb{sup -1}. The measurement of W + jets is compared to the Leading Order Alpgen + Pythia prediction and the Z + jets is compared to Sherpa and Pythia Monte Carlo. The Z p{sub T} measurement is also compared to Resbos + Photos predictions.

  1. CALCULATIONS FOR A MERCURY JET TARGET IN A SOLENOID MAGNET CAPTURE SYSTEM.

    SciTech Connect

    GALLARDO, J.; KAHN, S.; PALMER, R.B.; THIEBERGER, P.; WEGGEL, R.J.; MCDONALD, K.

    2001-06-18

    A mercury jet is being considered as the production target for a muon storage ring facility to produce an intense neutrino beam. A 20 T solenoid magnet that captures pions for muon production surrounds the mercury target. As the liquid metal jet enters or exits the field eddy currents are induced. We calculate the effects that a liquid metal jet experiences in entering and exiting the magnetic field for the magnetic configuration considered in the Neutrino Factory Feasibility Study II.

  2. Melting Curve of He4: No Sign of a Supersolid Transition down to 10mK

    NASA Astrophysics Data System (ADS)

    Todoshchenko, I. A.; Alles, H.; Bueno, J.; Junes, H. J.; Parshin, A. Ya.; Tsepelin, V.

    2006-10-01

    We have measured the melting curve of He4 in the temperature range from 10 to 400 mK with the accuracy of about 0.5μbar. Crystals of different quality show the expected T4 dependence in the range from 80 to 400 mK without any sign of the supersolid transition, and the coefficient is in excellent agreement with available data on the sound velocity in liquid He4 and on the Debye temperature of solid He4. Below 80 mK, we have observed a small deviation from T4 dependence, which, however, cannot be attributed to the supersolid transition, because instead of decrease the entropy of the solid rather remains constant, about 2.5×10-6 R.

  3. The neuroprotective action of dizocilpine (MK-801) in the rat middle cerebral artery occlusion model of focal ischaemia.

    PubMed Central

    Gill, R.; Brazell, C.; Woodruff, G. N.; Kemp, J. A.

    1991-01-01

    1. An acute model of focal ischaemia, which involves permanent occlusion of the middle cerebral artery of the rat with 4 h survival, was used to find the minimum effective plasma concentration of dizocilpine (MK-801) and to determine its dose-effect relationship. 2. MK-801 was administered at the time of occlusion and was given as an i.v. bolus followed by an infusion for 4 h to maintain a steady state plasma concentration of the drug throughout the study. MK-801 was given at 3 dose levels; 0.04 mg kg-1 i.v. bolus + 0.6 micrograms kg-1 min-1 infusion; 0.12 mg kg-1 i.v. bolus + 1.8 micrograms kg-1 min-1 infusion; 0.4 mg kg-1 i.v. bolus + 6 micrograms kg-1 min-1 infusion, which gave mean plasma levels over the 4 h of 8.0 ng ml-1, 18.9 ng ml-1 and 113.2 ng ml-1 respectively. 3. MK-801 at 8.0 ng ml-1 gave 10% reduction in the volume of ischaemic brain damage in the cerebral cortex which just reached significance. The middle dose of MK-801 (18.9 ng ml-1) gave a highly significant reduction in the volume of ischaemic brain damage in the cerebral cortex and hemisphere, volumes of ischaemic tissue being reduced by 60% and 50% compared to saline-treated animals, respectively. The highest plasma concentration of MK-801 (113.2 ng ml-1) resulted in a 35% reduction in the volume of hemispheric damage and a 40% reduction in the volume of cortical damage, which were significant.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:1912992

  4. Competitive (AP7) and non-competitive (MK-801) NMDA receptor antagonists differentially alter glucose utilization in rat cortex

    SciTech Connect

    Clow, D.W.; Lee, S.J.; Hammer, R.P. Jr. )

    1991-04-01

    The effects of D,L-2-amino-7-phosphonoheptanoic acid (AP7), a competitive N-methyl-D-aspartate (NMDA) receptor antagonist, and MK-801, a non-competitive NMDA receptor antagonist, on regional brain metabolism were studied in unanesthetized, freely moving rats by using the quantitative {sup 14}C2-deoxyglucose autoradiographic procedure. AP7 (338 or 901 mg/kg) produced a dose-dependent decrease of metabolic activity throughout most of the regions studied including sensory, motor, and limbic cortices. In contrast, MK-801 (0.1 or 1.0 mg/kg) resulted in a dose-dependent decrease of metabolic activity in sensory cortices, and an increase in limbic regions such as the hippocampal stratum lacunosum moleculare and entorhinal cortex. MK-801 also produced a biphasic response in agranular motor cortex, whereby the low dose increased while the high dose decreased labeling. In addition, MK-801 produced heterogeneous effects on regional cerebral metabolism in sensory cortices. Metabolic activity decreased in layer IV relative to layer Va following MK-801 treatment in primary somatosensory (SI) and visual (VI) cortices, suggesting a shift in activity from afferent fibers innervating layer IV to those innervating layer Va. MK-801 administration also decreased metabolic activity in granular SI relative to dysgranular SI, and in VI relative to secondary visual cortex (VII), thus providing a relative sparing of activity in dysgranular SI and VII. Thus, the non-competitive NMDA receptor antagonist suppressed activity from extrinsic neocortical sources, enhancing relative intracortical activity and stimulating limbic regions, while the competitive NMDA antagonist depressed metabolic activity in all cortical regions.

  5. Antiviral Activity and In Vitro Mutation Development Pathways of MK-6186, a Novel Nonnucleoside Reverse Transcriptase Inhibitor

    PubMed Central

    Lu, Meiqing; Felock, Peter J.; Munshi, Vandna; Hrin, Renee C.; Wang, Ying-Jie; Yan, Youwei; Munshi, Sanjeev; McGaughey, Georgia B.; Gomez, Robert; Anthony, Neville J.; Williams, Theresa M.; Grobler, Jay A.; Hazuda, Daria J.; McKenna, Philip M.; Miller, Michael D.

    2012-01-01

    MK-6186 is a novel nonnucleoside reverse transcriptase inhibitor (NNRTI) which displays subnanomolar potency against wild-type (WT) virus and the two most prevalent NNRTI-resistant RT mutants (K103N and Y181C) in biochemical assays. In addition, it showed excellent antiviral potency against K103N and Y181C mutant viruses, with fold changes (FCs) of less than 2 and 5, respectively. When a panel of 12 common NNRTI-associated mutant viruses was tested with MK-6186, only 2 relatively rare mutants (Y188L and V106I/Y188L) were highly resistant, with FCs of >100, and the remaining viruses showed FCs of <10. Furthermore, a panel of 96 clinical virus isolates with NNRTI resistance mutations was evaluated for susceptibility to NNRTIs. The majority (70%) of viruses tested displayed resistance to efavirenz (EFV), with FCs of >10, whereas only 29% of the mutant viruses displayed greater than 10-fold resistance to MK-6186. To determine whether MK-6186 selects for novel resistance mutations, in vitro resistance selections were conducted with one isolate each from subtypes A, B, and C under low-multiplicity-of-infection (MOI) conditions. The results showed a unique mutation development pattern in which L234I was the first mutation to emerge in the majority of the experiments. In resistance selection under high-MOI conditions with subtype B virus, V106A was the dominant mutation detected in the breakthrough viruses. More importantly, mutant viruses selected by MK-6186 showed FCs of <10 against EFV or etravirine (ETR), and the mutant viruses containing mutations selected by EFV or ETR were sensitive to MK-6186 (FCs of <10). PMID:22391531

  6. Models comparison for JET polarimeter data

    SciTech Connect

    Mazzotta, C.; Orsitto, F. P.; Giovannozzi, E.; Boboc, A.; Tudisco, O.; Zabeo, L.; Brombin, M.; Murari, A.

    2008-03-12

    A complete comparison between the theory and the measurements in polarimetry was done by using the Far Infrared Polarimeter at JET. More than 300 shots were analyzed, including a wide spectrum of JET scenarios in all critical conditions for polarimetry: high density, high and very low fields, high temperatures.This work is aimed at the demonstration of the robustness of the theoretical models for the JET polarimeter measurements in the perspective of using these models for ITER like plasma scenarios . In this context, an assessment was performed on how the line-integrated plasma density along the central vertical chord of FIR polarimeter could be evaluated using the Cotton-Mouton effect and its possible concrete use to correct fringe jumps of the interferometer.The models considered are: i) the rigorous numerical solution of the Stokes propagation equations, using dielectric tensor evaluated from JET equilibrium and Thomson scattering [1,2]; ii) two types of approximated solutions [2,3] and iii) the Guenther empirical model [4] that considers the mutual effect between Cotton-Mouton and Faraday rotation angle. The model calculations have been compared with polarimeter measurements for the Cotton-Mouton phase shift.The agreement with theory is satisfactory within the limits of experimental errors [3].

  7. Status on the Michigan-MIT ultra-cold polarized hydrogen jet target

    NASA Astrophysics Data System (ADS)

    Luppov, V. G.; Blinov, B. B.; Bywater, J. A.; Chin, S.; Churakov, V. V.; Court, G. R.; Kaufman, W. A.; Kleppner, D.; Krisch, A. D.; Melnik, Yu. M.; Muldavin, J. B.; Nurushev, T. S.; Price, J. S.; Prudkoglyad, A. F.; Raymond, R. S.; Shutov, V. B.; Stewart, J. A.

    1995-07-01

    Progress on the Mark-II ultra-cold polarized atomic hydrogen gas Jet target for the experiments NEPTUN-A and NEPTUN at UNK is presented. We describe the performance and the present status of different components of the jet.

  8. Study of the heavy flavour fractions in z+jets events from proton-antiproton collisions at energy = 1.96 TeV with the CDF II detector at the Tevatron collider

    SciTech Connect

    Mastrandrea, Paolo; /Siena U.

    2008-06-01

    at the end of 2008. In the meanwhile the only running accelerator able to provide collisions suitable for the search of the Higgs boson is the Tevatron at Fermilab, a proton-antiproton collider with a center of mass energy of 1.96 TeV working at 3 {center_dot} 10{sup 32}cm{sup -2}s{sup -1} peak luminosity. These features make the Tevatron able for the direct search of the Higgs boson in the 115-200 GeV mass range. Since the coupling of the Higgs boson is proportional to the masses of the particles involved, the decay in b{bar b} has the largest branching ratio for Higgs mass < 135 GeV and thus the events Z/W + b{bar b} are the main background to the Higgs signal in the most range favored by Standard Model fits. In this thesis a new technique to identify Heavy Flavour quarks inside high - P{sub T} jets is applied to events with a reconstructed Z boson to provide a measurement of the Z+b and Z+c inclusive cross sections. The study of these channels represent also a test of QCD in high transferred momentum regime, and can provide information on proton pdf. This new Heavy Flavour identication technique (tagger) provides an increased statistical separation between b, c and light flavours, using a new vertexing algorithm and a chain of artificial Neural Networks to exploit as much information as possible in each event. For this work I collaborated with the Universita di Roma 'La Sapienza' group working in the CDF II experiment at Tevatron, that has at first developed this tagger. After a brief theoretical introduction (chapter 1) and a description of the experimental apparatus (chapter 2), the tagger itself and its calibration procedure are described in chapter 3 and 4. The chapter 5 is dedicated to the event selection and the chapter 6 contains the results of the measurement and the study of the systematic errors.

  9. A study on L-asparaginase of Nocardia levis MK-VL_113.

    PubMed

    Kavitha, Alapati; Vijayalakshmi, Muvva

    2012-01-01

    An enzyme-based drug, L-asparaginase, was produced by Nocardia levis MK-VL_113 isolated from laterite soils of Guntur region. Cultural parameters affecting the production of L-asparaginase by the strain were optimized. Maximal yields of L-asparaginase were recorded from 3-day-old culture grown in modified asparagine-glycerol salts broth with initial pH 7.0 at temperature 30°C. Glycerol (2%) and yeast extract (1.5%) served as good carbon and nitrogen sources for L-asparaginase production, respectively. Cell-disrupting agents like EDTA slightly enhanced the productivity of L-asparaginase. Ours is the first paper on the production of L-asparaginase by N. levis. PMID:22619604

  10. A Study on L-Asparaginase of Nocardia levis MK-VL_113

    PubMed Central

    Kavitha, Alapati; Vijayalakshmi, Muvva

    2012-01-01

    An enzyme-based drug, L-asparaginase, was produced by Nocardia levis MK-VL_113 isolated from laterite soils of Guntur region. Cultural parameters affecting the production of L-asparaginase by the strain were optimized. Maximal yields of L-asparaginase were recorded from 3-day-old culture grown in modified asparagine-glycerol salts broth with initial pH 7.0 at temperature 30°C. Glycerol (2%) and yeast extract (1.5%) served as good carbon and nitrogen sources for L-asparaginase production, respectively. Cell-disrupting agents like EDTA slightly enhanced the productivity of L-asparaginase. Ours is the first paper on the production of L-asparaginase by N. levis. PMID:22619604

  11. Super-hot (T > 30 MK) thermal plasma in solar flares

    NASA Astrophysics Data System (ADS)

    Caspi, Amir

    2010-05-01

    The Sun offers a convenient nearby laboratory to study the physical processes of particle acceleration and impulsive energy release in magnetized plasmas that occur throughout the universe, from planetary magnetospheres to black hole accretion disks. Solar flares are the most powerful explosions in the solar system, releasing up to 1032-1033 ergs over only 100-1,000 seconds. These events can accelerate electrons up to hundreds of MeV and can heat plasma to tens of MK, exceeding ∼40 MK in the most intense flares. The accelerated electrons and the hot plasma each contain tens of percent of the total flare energy, indicating an intimate link between particle acceleration, plasma heating, and flare energy release. X-ray emission is the most direct signature of these processes; accelerated electrons emit hard X-ray bremsstrahlung as they collide with the ambient atmosphere, while hot plasma emits soft X-rays from both bremsstrahlung and excitation lines of highly-ionized atoms. The Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI) observes this emission from ∼3 keV to ∼17 MeV with unprecedented spectral, spatial, and temporal resolution, providing the most precise measurements of the X-ray flare spectrum and enabling the most accurate characterization of the X-ray-emitting hot and accelerated electron populations. RHESSI observations show that "super-hot" temperatures exceeding ∼30 MK are common in large flares but are achieved almost exclusively by X-class events and appear to be strictly associated with coronal magnetic field strengths exceeding ∼170 Gauss; these results suggest a direct link between the magnetic field and heating of super-hot plasma, and that super-hot flares may require a minimum threshold of field strength and overall flare intensity. Imaging and spectroscopic observations of the 2002 July 23 X4.8 event show that the superhot plasma is both spectrally and spatially distinct from the usual ∼10-20 MK plasma observed in nearly

  12. Design and construction of high-sensitivity, infrared bolometers for operation at 300 mK

    NASA Astrophysics Data System (ADS)

    Alsop, D. C.; Inman, C.; Lange, A. E.; Wibanks, T.

    1992-11-01

    The design and construction of 300-mK composite bolometers developed for millimeter-wave astronomical observations are described. Graphite fibers are used as the electrical leads for the thermistor to reduce the thermal conductance and heat capacity associated with the leads. A mechanical suspension made of Nylon fibers provides the required thermal conductance. Electrical noise equivalent powers below 1 x 10 exp -16 W/sq rt Hz have been achieved for detectors with thermal time constants of 11 ms. The detectors were installed in a millimeter-wave photometer and used to perform observations of the cosmic microwave background from a balloonborne platform. The flight performance was consistent with the measured laboratory properties.

  13. Josephson STM at mK temperatures: Coupling to the electronic environment

    NASA Astrophysics Data System (ADS)

    Dreyer, Michael; Dana, Rami; Liao, Wan-Ting; Lobb, Cris; Wellstood, Fred; Anderson, Bob

    Ultra-small Josephson junctions can couple to modes in the electronic environment. This leads to sub-gap peaks in the I(V) curve in addition to the phase diffuse supercurrent. The I(V) curve can - in principle - be explained by P(E) theory which describes the probability of tunneling at energy E. A recent study showed that antenna modes of the STM tips could be responsible for the observed sideband structures. In our case the explanation appears to be less simple. We employ a dual tip STM at a temperature of 30 mK. The I(V) spectra of the two tips show distinct patterns with only one shared mode. While the supercurrent branch for the ''inner'' tip is visible, it is obscured by a resonance for the ``outer'' tip. Possible causes and applications to other systems will be discussed. Support from NSF (DMR- 0605763) and Laboratory for Physical Sciences.

  14. Mechanical suppression: Modern technology applied to an old art. [MK 12 motors

    NASA Technical Reports Server (NTRS)

    Crockford, W. H.; Durney, T. E.; Scott, D. E.; Condon, J. A.

    1980-01-01

    Various suppressor configurations were analyzed and evaluated in an effort to reduce combustion instability and improved ballistic reproducibility of the MK 12 motor. A static firing test series of eight field return boosters featured two baseline motor firings with the existing suppressor in each motor, followed by two tests each of two different prototype suppressor designs. Results were analyzed and used to generate the design for the final two firings. Acoustic pressure pulsers were utilized to measure the damping effectiveness of the final design. The reduction in motor vibration and acoustic pressure levels between the baseline tests and the final two improved lightweight tests was significant. The average acceleration level of the motors equipped with flightweight rods was reduced to only 5% of the level in the unmodified motors; the average acoustic pressure level was reduced to 20%; maximum values were reduced to 6% for acceleration and 23% for pressure.

  15. [The Elektronika MK-52 programmable calculator in processing the results of biochemical studies].

    PubMed

    Dvorkin, V M

    1990-01-01

    The author reports the use of a new type of an Elektronika MK-52 programmed microcalculator for processing the results of biochemical studies. In contrast to other designs manufactured in this country, it is supplied with semifixed memory that permits storage of programs and data in the calculator after feeding switch off. Two new programs are suggested for estimating chorionic gonadotropin activity and for estimating liposome count in a preparation, and basic operations with the calculator are described as exemplified by these programs. The third program suggested by the author is intended for plotting various calibration straight lines; basing on this program the author demonstrates the use of memory enlargement units manufactured for this calculator. PMID:1699053

  16. Impulsively started incompressible turbulent jet

    SciTech Connect

    Witze, P O

    1980-10-01

    Hot-film anemometer measurements are presented for the centerline velocity of a suddenly started jet of air. The tip penetration of the jet is shown to be proportional to the square-root of time. A theoretical model is developed that assumes the transient jet can be characterized as a spherical vortex interacting with a steady-state jet. The model demonstrates that the ratio of nozzle radius to jet velocity defines a time constant that uniquely characterizes the behavior and similarity of impulsively started incompressible turbulent jets.

  17. Evaluation of prototype 100mK bolometric detector for Planck Surveyor

    NASA Astrophysics Data System (ADS)

    Sudiwala, R. V.; Maffei, B.; Griffin, M. J.; Haynes, C. V.; Ade, P. A. R.; Bhatia, R. S.; Turner, A. D.; Bock, J. J.; Lange, A. E.; Beeman, J. W.

    2000-04-01

    The High-Frequency Instrument (HFI) for the Planck Surveyor mission will measure anisotropies of the Cosmic Microwave Background (CMB) down to scales of 6 arcmin and to an accuracy of /ΔT/T=2×10-6. Channels ranging in frequency from 100 to 857GHz will use 100mK spider web bolometer detectors with NTD Ge thermistors. The detectors must be photon noise limited and fast enough to preserve signal information at the 1r.p.m. scan rate of the satellite. The prime low-frequency CMB channels at 143 and 217GHz are the most technically demanding owing to the lower background limited NEPs. For the 143GHz channel the requirements are that the time constant /τ<5.7 ms and the NEPbol <1.53×10-17 WHz-1/2 including contribution from amplifier noise. We present here thermal, electrical and optical data on a prototype detector which, although optimised for the 100GHz channel, satisfies most of the requirements of the more demanding 143GHz channel. The measurements are consistent with ideal thermal behaviour of the detector over the appropriate bias and temperature ranges for optimum performance. From optically blanked electrical measurements we determined the dependence of resistance and thermal conductance on temperature over a wide range, 70-200mK. The optical responsivity and NEP were measured under photon background conditions similar to those expected in flight. Measurements of speed of response as a function of bias at different temperatures allowed us to determine the variation of total heat capacity with temperature. Extrapolation of these data show that in principal performance for all the Planck HFI channels can be met.

  18. Postnatal exposure to MK801 induces selective changes in GAD67 or parvalbumin.

    PubMed

    Turner, Christopher Paul; DeBenedetto, Danielle; Ware, Emily; Stowe, Robert; Lee, Andrew; Swanson, John; Walburg, Caroline; Lambert, Alexandra; Lyle, Melissa; Desai, Priyanka; Liu, Chun

    2010-03-01

    Brain injury during the last trimester to the first 1-4 years in humans is now thought to trigger an array of intellectual and emotional problems later in life, including disorders such as schizophrenia. In adult schizophrenic brains, there is a specific loss of neurons that co-express glutamic acid decarboxylase-parvalbumin (GAD67-PV). Loss of this phenotype is thought to occur in mature animals previously exposed to N-methyl-D: -aspartate receptor (NMDAR) antagonists during late gestation or at postnatal day 7 (P7). However, in similarly treated animals, we have previously shown that GAD67 and PV are unaltered in the first 24 h. To more precisely define when changes in these markers first occur, we exposed rat pups (P7 or P6-P10) to the NMDAR antagonist MK801 and at P11 co-stained brain sections for GAD67 or PV. In the cingulate cortex, we found evidence for a reduction in PV (GAD67 levels were very low to undetectable). In contrast, in the somatosensory cortex, we found that expression of GAD67 was reduced, but PV remained stable. Further, repeated but not single doses of MK801 were necessary to see such changes. Thus, depending on the region, NMDAR antagonism appears to influence expression of PV or GAD67, but not both. These observations could not have been predicted by previous studies and raise important questions as to how the GAD67-PV phenotype is lost once animals reach maturity. More importantly, such differential effects may be of great clinical importance, given that cognitive deficits are seen in children exposed to anesthetics that act by blocking the NMDAR. PMID:19885653

  19. Measurements on the melting curve of ^4He down to 10 mK

    NASA Astrophysics Data System (ADS)

    Alles, Harry

    2007-03-01

    Recent discovery of a nonclassical rotational inertia in solid ^4He below about 0.2 K by Kim and Chan has initiated an intensive study on the properties of solid ^4He. As Kim and Chan have interpreted their observation as the evidence of supersolid behavior, we have decided to measure very accurately the melting curve of ^4He because, as the slope of the melting curve is proportional to the difference in the entropy of the liquid and solid phases, there should be an anomaly at the possible supersolid transition. We have measured the melting curve of ^4He with the accuracy of about 0.5 microbar with ^4He crystals which had various concentration of defects. All our samples showed only the expected T^4 dependence due to phonons without any sign of the supersolid transition in the temperature range of 80400 mK. Below 80 mK we observed a small deviation from T^4 dependence which, however, cannot be attributed to the supersolid transition because our recent measurements with the cell containing liquid sample only suggest that this deviation is by the temperature variation of properties of BeCu membrane of our capacitive pressure gauge. In addition to our published data with ^4He of natural purity [Phys. Rev. Lett. 97, 165302 (2006)] we also report our recent measurements of the melting curve with ultra pure ^4He (0.3 ppb of ^3He impurities). In these measurements we followed optically the shape of the sample crystals which had very good quality.

  20. Enhanced control and sensing for the REMOTEC ANDROS Mk VI robot. CRADA final report

    SciTech Connect

    Spelt, P.F.; Harvey, H.W.

    1998-08-01

    This Cooperative Research and Development Agreement (CRADA) between Lockheed Martin Energy Systems, Inc., and REMOTEC, Inc., explored methods of providing operator feedback for various work actions of the ANDROS Mk VI teleoperated robot. In a hazardous environment, an extremely heavy workload seriously degrades the productivity of teleoperated robot operators. This CRADA involved the addition of computer power to the robot along with a variety of sensors and encoders to provide information about the robot`s performance in and relationship to its environment. Software was developed to integrate the sensor and encoder information and provide control input to the robot. ANDROS Mk VI robots are presently used by numerous electric utilities to perform tasks in reactors where substantial exposure to radiation exists, as well as in a variety of other hazardous environments. Further, this platform has potential for use in a number of environmental restoration tasks, such as site survey and detection of hazardous waste materials. The addition of sensors and encoders serves to make the robot easier to manage and permits tasks to be done more safely and inexpensively (due to time saved in the completion of complex remote tasks). Prior research on the automation of mobile platforms with manipulators at Oak Ridge National Laboratory`s Center for Engineering Systems Advanced Research (CESAR, B&R code KC0401030) Laboratory, a BES-supported facility, indicated that this type of enhancement is effective. This CRADA provided such enhancements to a successful working teleoperated robot for the first time. Performance of this CRADA used the CESAR laboratory facilities and expertise developed under BES funding.

  1. Enhanced control & sensing for the REMOTEC ANDROS Mk VI robot. Final report

    SciTech Connect

    Spelt, P.F.; Harvey, H.W.

    1997-08-01

    This Cooperative Research and Development Agreement (CRADA) between Lockheed Marietta Energy Systems, Inc., and REMOTEC, Inc., explored methods of providing operator feedback for various work actions of the ANDROS Mk VI teleoperated robot. In a hazardous environment, an extremely heavy workload seriously degrades the productivity of teleoperated robot operators. This CRADA involved the addition of computer power to the robot along with a variety of sensors and encoders to provide information about the robot`s performance in and relationship to its environment. Software was developed to integrate the sensor and encoder information and provide control input to the robot. ANDROS Mk VI robots are presently used by numerous electric utilities to perform tasks in reactors where substantial exposure to radiation exists, as well as in a variety of other hazardous environments. Further, this platform has potential for use in a number of environmental restoration tasks, such as site survey and detection of hazardous waste materials. The addition of sensors and encoders serves to make the robot easier to manage and permits tasks to be done more safely and inexpensively (due to time saved in the completion of complex remote tasks). Prior research on the automation of mobile platforms with manipulators at Oak Ridge National Laboratory`s Center for Engineering Systems Advanced Research (CESAR, B&R code KC0401030) Laboratory, a BES-supported facility, indicated that this type of enhancement is effective. This CRADA provided such enhancements to a successful working teleoperated robot for the first time. Performance of this CRADA used the CESAR laboratory facilities and expertise developed under BES funding.

  2. Scanning Tunneling Microscopy of Dirac Fermions at mK Temperatures

    NASA Astrophysics Data System (ADS)

    Stroscio, Joseph

    2011-03-01

    Since the beginning of the last century new frontiers in physics have emerged when advances in instrumentation achieved lower experimental operating temperatures. Notable examples include the discovery of superconductivity and the integer and fractional quantum Hall effects. New experimental techniques are continually adapted in order to meet new experimental challenges. A case in point is scanning tunneling microscopy (STM) which has seen a wealth of new measurements emerge as cryogenic STM instruments have been developed in the last two decades. In this talk I describe the design, development and performance of a scanning probe microscopy facility operating at a base temperature of 10 mK in magnetic fields up to 15 T. The microscope is cooled by a custom designed, fully ultra-high vacuum (UHV) compatible dilution refrigerator (DR) and is capable of in-situ tip and sample exchange. Sub-picometer stability at the tip-sample junction is achieved through three independent vibration isolation stages and careful design of the dilution refrigerator. The system can be connected to, or disconnected from, a network of interconnected auxiliary UHV chambers used for sample and probe tip preparation. Current measurements are focusing on Dirac fermions in graphene and in topological insulators. The history of the fractional quantum Hall states in semiconductor heterostructures suggests that studying graphene at lower temperatures and higher magnetic fields may reveal new quantum phases of matter. Scanning tunneling spectroscopy of graphene at mK temperatures reveals the detailed structure of the degenerate Landau levels in graphene, resolving the full quartet of states corresponding to the lifting of the spin and valley dengeneracies. When the Fermi level lies inside the four-fold Landau manifold, significant electron correlation effects result in enhanced valley splitting and spin splitting. New many-body states are observed at fractional filling factors of 7/2, 9/2, and 11/2.

  3. Ram-jet Performance

    NASA Technical Reports Server (NTRS)

    Cervenko, A. J.; Friedman, R.

    1956-01-01

    The ram jet is basically one of the most dimple types of aircraft engine. It consists only of an inlet diffuser, a combustion system, and an exit nozzle. A typical ram-jet configuration is shown in figure 128. The engine operates on the Brayton cycle, and ideal cycle efficiency depends only on the ratio of engine to ambient pressure. The increased, engine pressures are obtained by ram action alone, and for this reason the ram jet has zero thrust at zero speed. Therefore, ram-jet-powered aircraft must be boosted to flight speeds close to a Mach number of 1.0 before appreciable thrust is generated by the engine. Since pressure increases are obtained by ram action alone, combustor-inlet pressures and temperatures are controlled by the flight speed, the ambient atmospheric condition, and by the efficiency of the inlet diffuser. These pressures and temperatures, as functions of flight speed and altitude, are shown in figure 129 for the NACA standard atmosphere and for practical values of diffuser efficiency. It can be seen that very wide ranges of combustor-inlet temperatures and pressures may be encountered over the ranges of flight velocity and altitude at which ram jets may be operated. Combustor-inlet temperatures from 500 degrees to 1500 degrees R and inlet pressures from 5 to 100 pounds per square inch absolute represent the approximate ranges of interest in current combustor development work. Since the ram jet has no moving parts in the combustor outlet, higher exhaust-gas temperatures than those used in current turbojets are permissible. Therefore, fuel-air ratios equivalent to maximum rates of air specific impulse or heat release can be used, and, for hydrocarbon fuels, this weight ratio is about 0.070. Lower fuel-air ratios down to about 0.015 may also be required to permit efficient cruise operation. This fuel-air-ratio range of 0.015 to 0.070 used in ram jets can be compared with the fuel-air ratios up to 0.025 encountered in current turbojets. Ram-jet

  4. The Twin Jet Nebula

    NASA Technical Reports Server (NTRS)

    1997-01-01

    M2-9 is a striking example of a 'butterfly' or a bipolar planetary nebula. Another more revealing name might be the 'Twin Jet Nebula.' If the nebula is sliced across the star, each side of it appears much like a pair of exhausts from jet engines. Indeed, because of the nebula's shape and the measured velocity of the gas, in excess of 200 miles per second, astronomers believe that the description as a super-super-sonic jet exhaust is quite apt. This is much the same process that takes place in a jet engine: The burning and expanding gases are deflected by the engine walls through a nozzle to form long, collimated jets of hot air at high speeds. M2-9 is 2,100 light-years away in the constellation Ophiucus. The observation was taken Aug. 2, 1997 by the Hubble telescope's Wide Field and Planetary Camera 2. In this image, neutral oxygen is shown in red, once-ionized nitrogen in green, and twice-ionized oxygen in blue.

  5. Jet penetration in glass

    SciTech Connect

    Moran, B.; Glenn, L.A.; Kusubov, A.

    1991-05-01

    We describe a phenomenological model which accounts for the mechanical response of glass to intense impulsive loading. An important aspect of this response is the dilatancy accompanying fracture. We have also conducted a number of experiments with 38.1-mm diameter precision shaped charges to establish the performance against various targets and to allow evaluation of our model. At 3 charge diameters standoff, the data indicate that both virgin and damaged glass offer better (Bernoulli-scaled) resistance to penetration than either of 4340 steel, or 6061-T6 aluminum alloy. Time-resolved measurements indicate two distinct phases of jet penetration in glass: An initial hydrodynamic phase, and a second phase characterized by a slower penetration velocity. Our calculations show that at early time, a crater is formed around the jet and only the tip of the undisturbed jet interacts with the glass. At late time the glass has collapsed on the jet and degraded penetration continues via a disturbed and fragmented jet.

  6. Sweeping Jet Optimization Studies

    NASA Technical Reports Server (NTRS)

    Melton, LaTunia Pack; Koklu, Mehti; Andino, Marlyn; Lin, John C.; Edelman, Louis

    2016-01-01

    Progress on experimental efforts to optimize sweeping jet actuators for active flow control (AFC) applications with large adverse pressure gradients is reported. Three sweeping jet actuator configurations, with the same orifice size but di?erent internal geometries, were installed on the flap shoulder of an unswept, NACA 0015 semi-span wing to investigate how the output produced by a sweeping jet interacts with the separated flow and the mechanisms by which the flow separation is controlled. For this experiment, the flow separation was generated by deflecting the wing's 30% chord trailing edge flap to produce an adverse pressure gradient. Steady and unsteady pressure data, Particle Image Velocimetry data, and force and moment data were acquired to assess the performance of the three actuator configurations. The actuator with the largest jet deflection angle, at the pressure ratios investigated, was the most efficient at controlling flow separation on the flap of the model. Oil flow visualization studies revealed that the flow field controlled by the sweeping jets was more three-dimensional than expected. The results presented also show that the actuator spacing was appropriate for the pressure ratios examined.

  7. B-jets and z + b-jets at CDF

    SciTech Connect

    Jeans, Daniel; /Rome U.

    2006-06-01

    The authors present CDF cross-section measurements for the inclusive production of b jets and the production of b jets in association with a Z{sup 0} boson. Both measurements are in reasonable agreement with NLO QCD predictions.

  8. Effect of finite container size on granular jet formation.

    PubMed

    von Kann, Stefan; Joubaud, Sylvain; Caballero-Robledo, Gabriel A; Lohse, Detlef; van der Meer, Devaraj

    2010-04-01

    When an object is dropped into a bed of fine, loosely packed sand, a surprisingly energetic jet shoots out of the bed. In this work we study the effect that boundaries have on the granular jet formation. We did this by (i) decreasing the depth of the sand bed and (ii) reducing the container diameter to only a few ball diameters. These confinements change the behavior of the ball inside the bed, the void collapse, and the resulting jet height and shape. We map the parameter space of impact with Froude number, ambient pressure, and container dimensions as parameters. From these results we propose an explanation for the thick-thin structure of the jet reported by several groups ([J. R. Royer, Nat. Phys. 1, 164 (2005)], [G. Caballero, Phys. Rev. Lett. 99, 018001 (2007)], and [J. O. Marston, Phys. Fluids 20, 023301 (2008)]). PMID:20481716

  9. Cleaning of ITO glass with carbon dioxide snow jet spray

    NASA Astrophysics Data System (ADS)

    Li, Jun-jian; Qi, Tong; Li, Shu-lin; Zhao, Guang

    2007-12-01

    ITO glass cleaning is LCD, OLED and other flat panel display industry's key technologies. At present, the usual wet cleaning technology consumes large amount of water and chemicals, and produces a large amount of contaminant venting. CO II snow jet spray cleaning has been successfully applied to cleaning the surface of semiconductor chip, vacuum devices and space telescopes. Surface cleaning of indium tin oxide (ITO) film was carried out with carbon dioxide snow jet treatment .Based on the measurements of the contact angles, X-ray photoelectron spectroscopy and scanning electron microscopy (SEM) ,the influence of carbon dioxide snow jet treatment on surface cleaning of indium tin Oxide film was investigated and compared with the samples of low frequency immersion ultrasonic cleaning. Experimental data show that the carbon dioxide snow jet treatment effectively removes particulate and hydrocarbon on ITO surface.

  10. Aeroacoustic Experiments with Twin Jets

    NASA Technical Reports Server (NTRS)

    Bozak, Richard F.; Henderson, Brenda S.

    2012-01-01

    While the noise produced by a single jet is azimuthally symmetric, multiple jets produce azimuthally varying far-field noise. The ability of one jet to shield another reduces the noise radiated in the plane of the jets, while often increasing the noise radiated out of the plane containing the jets. The present study investigates the shielding potential of twin jet configurations over subsonic and over-expanded supersonic jet conditions with simulated forward flight. The experiments were conducted with 2 in. throat diameter nozzles at four jet spacings from 2.6d to 5.5d in center-to-center distance, where d is the nozzle throat diameter. The current study found a maximum of 3 dB reduction in overall sound pressure level relative to two incoherent jets in the peak jet noise direction in the plane containing the jets. However, an increase of 3 dB was found perpendicular to the plane containing the jets. In the sideline direction, shielding is observed for all jet spacings in this study.

  11. Renewable jet fuel.

    PubMed

    Kallio, Pauli; Pásztor, András; Akhtar, M Kalim; Jones, Patrik R

    2014-04-01

    Novel strategies for sustainable replacement of finite fossil fuels are intensely pursued in fundamental research, applied science and industry. In the case of jet fuels used in gas-turbine engine aircrafts, the production and use of synthetic bio-derived kerosenes are advancing rapidly. Microbial biotechnology could potentially also be used to complement the renewable production of jet fuel, as demonstrated by the production of bioethanol and biodiesel for piston engine vehicles. Engineered microbial biosynthesis of medium chain length alkanes, which constitute the major fraction of petroleum-based jet fuels, was recently demonstrated. Although efficiencies currently are far from that needed for commercial application, this discovery has spurred research towards future production platforms using both fermentative and direct photobiological routes. PMID:24679258

  12. JetStar

    NASA Technical Reports Server (NTRS)

    1982-01-01

    In this photo of the C-140 JetStar on the Dryden Ramp, a subscale propeller has been fitted to the upper fuselage of the aircraft. NASA's Dryden Flight Research Facility, in co-operation with the Lewis Research Center, investigated the acoustic characteristics of a series of subscale advanced design propellors in the early eighties. These propellors were designed to rotate at a tip speed faster than the speed of sound. They are, in effect, a 'swept back wing' version of a propellor. The tests were conducted on Dryden's C-140 Jetstar, seen here on the ramp at Dryden in Edwards, California. The JetStar was modified with the installation of an air turbine drive system. The drive motor, with a 24 inch test propellor, was mounted in a pylon atop the JetStar. The JetStar was equipped with an array of 28 microphones flush-mounted in the fuselage of the aircraft beneath the propellor. Microphones mounted on the wings and on accompanying chase aircraft provided far-field acoustic data. In the 1960s, the same JetStar was equipped with an electronic variable stability flight control system. Called then a General Purpose Airborne Simulator (GPAS), the aircraft could duplicate the flight characteristics of a wide variety of advanced aircraft and was used for supersonic transport and general aviation research and as a training and support system for Space Shuttle Approach and Landing Tests at Dryden in 1977. In 1985, the JetStar's wings were modified with suction and spray devices in a laminar (smooth) air flow program to study ways of improving the flow of air over the wings of airliners. The program also studied ways of reducing the collection of ice and insects on airliner wings.

  13. Measurement of the Single Top Quark Cross Section in the Lepton Plus Jets Final State in Proton-Antiproton Collisions at a Center of Mass Energy of 1.96 TeV Using the CDF II Detector

    SciTech Connect

    Wu, Zhenbin

    2012-01-01

    We present a measurement of the single top quark cross section in the lepton plus jets final state using an integrated luminosity corresponding to 7.5 fb-1 of p\\bar p collision data collected by the Collider Detector at Fermilab. The single top candidate events are identified by the signature of a charged lepton, large missing transverse energy, and two or three jets with at least one of them identified as originating from a bottom quark. A new Monte Carlo generator POWHEG is used to model the single top quark production processes, which include s-channel, t-channel, and Wt-channel. A neural network multivariate method is exploited to discriminate the single top quark signal from the comparatively large backgrounds. We measure a single top production cross section of $3.04^{+0.57}_{-0.53} (\\mathrm{stat.~+~syst.})$ pb assuming $m_{\\rm top}=172.5$~GeV/$c^2$. In addition, we extract the CKM matrix element value $|V_{tb}|=0.96\\pm 0.09~(\\mathrm{stat.~+~syst.})\\ ± 0.05~(\\mathrm{theory})$ and set a lower limit of $|V_{tb}|>0.78$ at the 95% credibility level.

  14. Measurement of inclusive jet cross sections in Z/gamma*(-->e+e-) + jets production in pp[over ] collisions at square root s = 1.96 TeV.

    PubMed

    Aaltonen, T; Adelman, J; Akimoto, T; Albrow, M G; Alvarez González, B; Amerio, S; Amidei, D; Anastassov, A; Annovi, A; Antos, J; Aoki, M; Apollinari, G; Apresyan, A; Arisawa, T; Artikov, A; Ashmanskas, W; Attal, A; Aurisano, A; Azfar, F; Azzi-Bacchetta, P; Azzurri, P; Bacchetta, N; Badgett, W; Barbaro-Galtieri, A; Barnes, V E; Barnett, B A; Baroiant, S; Bartsch, V; Bauer, G; Beauchemin, P-H; Bedeschi, F; Bednar, P; Behari, S; Bellettini, G; Bellinger, J; Belloni, A; Benjamin, D; Beretvas, A; Beringer, J; Berry, T; Bhatti, A; Binkley, M; Bisello, D; Bizjak, I; Blair, R E; Blocker, C; Blumenfeld, B; Bocci, A; Bodek, A; Boisvert, V; Bolla, G; Bolshov, A; Bortoletto, D; Boudreau, J; Boveia, A; Brau, B; Bridgeman, A; Brigliadori, L; Bromberg, C; Brubaker, E; Budagov, J; Budd, H S; Budd, S; Burkett, K; Busetto, G; Bussey, P; Buzatu, A; Byrum, K L; Cabrera, S; Campanelli, M; Campbell, M; Canelli, F; Canepa, A; Carlsmith, D; Carosi, R; Carrillo, S; Carron, S; Casal, B; Casarsa, M; Castro, A; Catastini, P; Cauz, D; Cavalli-Sforza, M; Cerri, A; Cerrito, L; Chang, S H; Chen, Y C; Chertok, M; Chiarelli, G; Chlachidze, G; Chlebana, F; Cho, K; Chokheli, D; Chou, J P; Choudalakis, G; Chuang, S H; Chung, K; Chung, W H; Chung, Y S; Ciobanu, C I; Ciocci, M A; Clark, A; Clark, D; Compostella, G; Convery, M E; Conway, J; Cooper, B; Copic, K; Cordelli, M; Cortiana, G; Crescioli, F; Cuenca Almenar, C; Cuevas, J; Culbertson, R; Cully, J C; Dagenhart, D; Datta, M; Davies, T; de Barbaro, P; De Cecco, S; Deisher, A; De Lentdecker, G; De Lorenzo, G; Dell'orso, M; Demortier, L; Deng, J; Deninno, M; De Pedis, D; Derwent, P F; Di Giovanni, G P; Dionisi, C; Di Ruzza, B; Dittmann, J R; D'Onofrio, M; Donati, S; Dong, P; Donini, J; Dorigo, T; Dube, S; Efron, J; Erbacher, R; Errede, D; Errede, S; Eusebi, R; Fang, H C; Farrington, S; Fedorko, W T; Feild, R G; Feindt, M; Fernandez, J P; Ferrazza, C; Field, R; Flanagan, G; Forrest, R; Forrester, S; Franklin, M; Freeman, J C; Furic, I; Gallinaro, M; Galyardt, J; Garberson, F; Garcia, J E; Garfinkel, A F; Gerberich, H; Gerdes, D; Giagu, S; Giakoumopolou, V; Giannetti, P; Gibson, K; Gimmell, J L; Ginsburg, C M; Giokaris, N; Giordani, M; Giromini, P; Giunta, M; Glagolev, V; Glenzinski, D; Gold, M; Goldschmidt, N; Golossanov, A; Gomez, G; Gomez-Ceballos, G; Goncharov, M; González, O; Gorelov, I; Goshaw, A T; Goulianos, K; Gresele, A; Grinstein, S; Grosso-Pilcher, C; Grundler, U; Guimaraes da Costa, J; Gunay-Unalan, Z; Haber, C; Hahn, K; Hahn, S R; Halkiadakis, E; Hamilton, A; Han, B-Y; Han, J Y; Handler, R; Happacher, F; Hara, K; Hare, D; Hare, M; Harper, S; Harr, R F; Harris, R M; Hartz, M; Hatakeyama, K; Hauser, J; Hays, C; Heck, M; Heijboer, A; Heinemann, B; Heinrich, J; Henderson, C; Herndon, M; Heuser, J; Hewamanage, S; Hidas, D; Hill, C S; Hirschbuehl, D; Hocker, A; Hou, S; Houlden, M; Hsu, S-C; Huffman, B T; Hughes, R E; Husemann, U; Huston, J; Incandela, J; Introzzi, G; Iori, M; Ivanov, A; Iyutin, B; James, E; Jayatilaka, B; Jeans, D; Jeon, E J; Jindariani, S; Johnson, W; Jones, M; Joo, K K; Jun, S Y; Jung, J E; Junk, T R; Kamon, T; Kar, D; Karchin, P E; Kato, Y; Kephart, R; Kerzel, U; Khotilovich, V; Kilminster, B; Kim, D H; Kim, H S; Kim, J E; Kim, M J; Kim, S B; Kim, S H; Kim, Y K; Kimura, N; Kirsch, L; Klimenko, S; Klute, M; Knuteson, B; Ko, B R; Koay, S A; Kondo, K; Kong, D J; Konigsberg, J; Korytov, A; Kotwal, A V; Kraus, J; Kreps, M; Kroll, J; Krumnack, N; Kruse, M; Krutelyov, V; Kubo, T; Kuhlmann, S E; Kuhr, T; Kulkarni, N P; Kusakabe, Y; Kwang, S; Laasanen, A T; Lai, S; Lami, S; Lammel, S; Lancaster, M; Lander, R L; Lannon, K; Lath, A; Latino, G; Lazzizzera, I; Lecompte, T; Lee, J; Lee, J; Lee, Y J; Lee, S W; Lefèvre, R; Leonardo, N; Leone, S; Levy, S; Lewis, J D; Lin, C; Lin, C S; Linacre, J; Lindgren, M; Lipeles, E; Lister, A; Litvintsev, D O; Liu, T; Lockyer, N S; Loginov, A; Loreti, M; Lovas, L; Lu, R-S; Lucchesi, D; Lueck, J; Luci, C; Lujan, P; Lukens, P; Lungu, G; Lyons, L; Lys, J; Lysak, R; Lytken, E; Mack, P; Macqueen, D; Madrak, R; Maeshima, K; Makhoul, K; Maki, T; Maksimovic, P; Malde, S; Malik, S; Manca, G; Manousakis, A; Margaroli, F; Marino, C; Marino, C P; Martin, A; Martin, M; Martin, V; Martínez, M; Martínez-Ballarín, R; Maruyama, T; Mastrandrea, P; Masubuchi, T; Mattson, M E; Mazzanti, P; McFarland, K S; McIntyre, P; McNulty, R; Mehta, A; Mehtala, P; Menzemer, S; Menzione, A; Merkel, P; Mesropian, C; Messina, A; Miao, T; Miladinovic, N; Miles, J; Miller, R; Mills, C; Milnik, M; Mitra, A; Mitselmakher, G; Miyake, H; Moed, S; Moggi, N; Moon, C S; Moore, R; Morello, M; Movilla Fernandez, P; Mülmenstädt, J; Mukherjee, A; Muller, Th; Mumford, R; Murat, P; Mussini, M; Nachtman, J; Nagai, Y; Nagano, A; Naganoma, J; Nakamura, K; Nakano, I; Napier, A; Necula, V; Neu, C; Neubauer, M S; Nielsen, J; Nodulman, L; Norman, M; Norniella, O; Nurse, E; Oh, S H; Oh, Y D; Oksuzian, I; Okusawa, T; Oldeman, R; Orava, R; Osterberg, K; Pagan Griso, S; Pagliarone, C; Palencia, E; Papadimitriou, V; Papaikonomou, A; Paramonov, A A; Parks, B; Pashapour, S; Patrick, J; Pauletta, G; Paulini, M; Paus, C; Pellett, D E; Penzo, A; Phillips, T J; Piacentino, G; Piedra, J; Pinera, L; Pitts, K; Plager, C; Pondrom, L; Portell, X; Poukhov, O; Pounder, N; Prakoshyn, F; Pronko, A; Proudfoot, J; Ptohos, F; Punzi, G; Pursley, J; Rademacker, J; Rahaman, A; Ramakrishnan, V; Ranjan, N; Redondo, I; Reisert, B; Rekovic, V; Renton, P; Rescigno, M; Richter, S; Rimondi, F; Ristori, L; Robson, A; Rodrigo, T; Rogers, E; Rolli, S; Roser, R; Rossi, M; Rossin, R; Roy, P; Ruiz, A; Russ, J; Rusu, V; Saarikko, H; Safonov, A; Sakumoto, W K; Salamanna, G; Saltó, O; Santi, L; Sarkar, S; Sartori, L; Sato, K; Savoy-Navarro, A; Scheidle, T; Schlabach, P; Schmidt, E E; Schmidt, M A; Schmidt, M P; Schmitt, M; Schwarz, T; Scodellaro, L; Scott, A L; Scribano, A; Scuri, F; Sedov, A; Seidel, S; Seiya, Y; Semenov, A; Sexton-Kennedy, L; Sfyria, A; Shalhout, S Z; Shapiro, M D; Shears, T; Shepard, P F; Sherman, D; Shimojima, M; Shochet, M; Shon, Y; Shreyber, I; Sidoti, A; Sinervo, P; Sisakyan, A; Slaughter, A J; Slaunwhite, J; Sliwa, K; Smith, J R; Snider, F D; Snihur, R; Soderberg, M; Soha, A; Somalwar, S; Sorin, V; Spalding, J; Spinella, F; Spreitzer, T; Squillacioti, P; Stanitzki, M; St Denis, R; Stelzer, B; Stelzer-Chilton, O; Stentz, D; Strologas, J; Stuart, D; Suh, J S; Sukhanov, A; Sun, H; Suslov, I; Suzuki, T; Taffard, A; Takashima, R; Takeuchi, Y; Tanaka, R; Tecchio, M; Teng, P K; Terashi, K; Thom, J; Thompson, A S; Thompson, G A; Thomson, E; Tipton, P; Tiwari, V; Tkaczyk, S; Toback, D; Tokar, S; Tollefson, K; Tomura, T; Tonelli, D; Torre, S; Torretta, D; Tourneur, S; Trischuk, W; Tu, Y; Turini, N; Ukegawa, F; Uozumi, S; Vallecorsa, S; van Remortel, N; Varganov, A; Vataga, E; Vázquez, F; Velev, G; Vellidis, C; Veszpremi, V; Vidal, M; Vidal, R; Vila, I; Vilar, R; Vine, T; Vogel, M; Volobouev, I; Volpi, G; Würthwein, F; Wagner, P; Wagner, R G; Wagner, R L; Wagner-Kuhr, J; Wagner, W; Wakisaka, T; Wallny, R; Wang, S M; Warburton, A; Waters, D; Weinberger, M; Wester, W C; Whitehouse, B; Whiteson, D; Wicklund, A B; Wicklund, E; Williams, G; Williams, H H; Wilson, P; Winer, B L; Wittich, P; Wolbers, S; Wolfe, C; Wright, T; Wu, X; Wynne, S M; Yagil, A; Yamamoto, K; Yamaoka, J; Yamashita, T; Yang, C; Yang, U K; Yang, Y C; Yao, W M; Yeh, G P; Yoh, J; Yorita, K; Yoshida, T; Yu, G B; Yu, I; Yu, S S; Yun, J C; Zanello, L; Zanetti, A; Zaw, I; Zhang, X; Zheng, Y; Zucchelli, S

    2008-03-14

    Inclusive jet cross sections in Z/gamma* events, with Z/gamma* decaying into an electron-positron pair, are measured as a function of jet transverse momentum and jet multiplicity in pp[over ] collisions at square root s = 1.96 TeV with the upgraded Collider Detector at Fermilab in run II, based on an integrated luminosity of 1.7 fb(-1). The measurements cover the rapidity region |y(jet)|<2.1 and the transverse momentum range p(T)(jet)>30 GeV/c. Next-to-leading order perturbative QCD predictions are in good agreement with the measured cross sections. PMID:18352174

  15. Impact of a viscoelastic jet

    NASA Astrophysics Data System (ADS)

    Lhuissier, Henri; Néel, Baptiste; Limat, Laurent

    2014-11-01

    A jet of a Newtonian liquid impacting onto a wall at right angle spreads as a thin liquid sheet which preserves the radial symmetry of the jet. We observe that for a viscoelastic jet (solution of polyethylene glycol in water) this symmetry can break: close to the wall, the jet cross-section is faceted and radial steady liquid films (membranes) form, which connect the cross-section vertices to the sheet. The number of membranes increases with increasing viscoelastic relaxation time of the solution, but also with increasing jet velocity and decreasing distance from the jet nozzle to the wall. A mechanism for this surprising destabilization of the jet, which develops perpendicularly to the direction expected for a buckling mechanism, is presented that explains these dependences. The large-scale consequences of the jet destabilization on the sheet spreading and fragmentation, which show through the faceting of hydraulic jumps and suspended (Savart) sheets, will also be discussed.

  16. Jet Shockwaves Produce Gamma Rays

    NASA Video Gallery

    Theorists believe that GRB jets produce gamma rays by two processes involving shock waves. Shells of material within the jet move at different speeds and collide, generating internal shock waves th...

  17. Jet pump assisted artery

    NASA Technical Reports Server (NTRS)

    1975-01-01

    A procedure for priming an arterial heat pump is reported; the procedure also has a means for maintaining the pump in a primed state. This concept utilizes a capillary driven jet pump to create the necessary suction to fill the artery. Basically, the jet pump consists of a venturi or nozzle-diffuser type constriction in the vapor passage. The throat of this venturi is connected to the artery. Thus vapor, gas, liquid, or a combination of the above is pumped continuously out of the artery. As a result, the artery is always filled with liquid and an adequate supply of working fluid is provided to the evaporator of the heat pipe.

  18. Astrophysical jet experiments

    NASA Astrophysics Data System (ADS)

    Gregory, C. D.; Loupias, B.; Waugh, J.; Barroso, P.; Bouquet, S.; Brambrink, E.; Dono, S.; Falize, E.; Howe, J.; Kuramitsu, Y.; Kodama, R.; Koenig, M.; Michaut, C.; Myers, S.; Nazarov, W.; Notley, M. M.; Oya, A.; Pikuz, S.; Rabec le Gloahec, M.; Sakawa, Y.; Spindloe, C.; Streeter, M.; Wilson, L. A.; Woolsey, N. C.

    2008-12-01

    In this paper, three different experimental configurations designed to study jet propagation physics are presented. Each configuration uses a different target design: conical dimples in solid surfaces, hollow cones filled with foam and angled thin foils. When irradiated with a laser, these targets result in the launching of a plasma jet, the properties of which can be controlled by judicious choices of the target and laser parameters. Experimental results from these targets are shown, and the physics which may be studied with each of these targets is briefly discussed.

  19. A Randomized, Double-Blind, Crossover Comparison of MK-0929 and Placebo in the Treatment of Adults with ADHD

    ERIC Educational Resources Information Center

    Rivkin, Anna; Alexander, Robert C.; Knighton, Jennifer; Hutson, Pete H.; Wang, Xiaojing J.; Snavely, Duane B.; Rosah, Thomas; Watt, Alan P.; Reimherr, Fred W.; Adler, Lenard A.

    2012-01-01

    Objective: Preclinical models, receptor localization, and genetic linkage data support the role of D4 receptors in the etiology of ADHD. This proof-of-concept study was designed to evaluate MK-0929, a selective D4 receptor antagonist as treatment for adult ADHD. Method: A randomized, double-blind, placebo-controlled, crossover study was conducted…

  20. NMDA ANTAGONIST MK-801 SUPPRESSES BEHAVIORAL SEIZURES, AUGMENTS AFTERDISCHARGES, BUT DOES NOT BLOCK DEVELOPMENT OF PERFORANT PATH KINDLING

    EPA Science Inventory

    The role of the N-methyl-d-aspartate (NMDA) in the development and expression of kindled seizures was assessed using a crossover design. ats were stimulated once daily in the perforant path for 10 consecutive days following administration of saline or the NMDA antagonist MK-801 (...

  1. White matter injuries induced by MK-801 in a mouse model of schizophrenia based on NMDA antagonism.

    PubMed

    Xiu, Yun; Kong, Xiang-Ru; Zhang, Lei; Qiu, Xuan; Chao, Feng-Lei; Peng, Chao; Gao, Yuan; Huang, Chun-Xia; Wang, San-Rong; Tang, Yong

    2014-08-01

    The etiology of schizophrenia (SZ) is complex and largely unknown. Neuroimaging and postmortem studies have suggested white matter disturbances in SZ. In the present study, we tested the white matter deficits hypothesis of SZ using a mouse model of SZ induced by NMDA receptor antagonist MK-801. We found that mice with repeated chronic MK-801 administration showed increased locomotor activity in the open field test, less exploration of a novel environment in the hole-board test, and increased anxiety in the elevated plus maze but no impairments were observed in coordination or motor function on accelerating rota-rod. The total white matter volume and corpus callosum volume in mice treated with MK-801 were significantly decreased compared to control mice treated with saline. Myelin basic protein and 2', 3'-cyclic nucleotide 3'-phosphodiesterase were also significantly decreased in the mouse model of SZ. Furthermore, we observed degenerative changes of myelin sheaths in the mouse model of SZ. These results provide further evidence of white matter deficits in SZ and indicate that the animal model of SZ induced by MK-801 is a useful model to investigate mechanisms underlying white matter abnormalities in SZ. PMID:24788877

  2. Documentation for the machine-readable version of the MK classification extension catalogue Morris-Kennedy (1983)

    NASA Technical Reports Server (NTRS)

    Warren, W. H., Jr.

    1983-01-01

    Detailed descriptions of the contents and formats of the data and reference files of the machine-readable catalog are provided. The catalog contains MK classifications published in the literature since the issuance of the La Plata catalog of C. Jaschek et al., (1964). The bibliographical references are also given.

  3. Synthesis of [(11)C]MK-1064 as a new PET radioligand for imaging of orexin-2 receptor.

    PubMed

    Gao, Mingzhang; Wang, Min; Zheng, Qi-Huang

    2016-08-01

    The reference standard MK-1064 {5″-chloro-N-((5,6-dimethoxypyridin-2-yl)methyl)-[2,2':5',3″-terpyridine]-3'-carboxamide} was synthesized from methyl 2-chloro-5-iodonicotinate and 5-(chloropyridin-3-yl)boronic acid in 4 steps with 33% overall chemical yield. The precursor desmethyl-MK-1064 {5″-chloro-N-((5-hydroxy-6-methoxypyridin-2-yl)methyl)-[2,2':5',3″-terpyridine]-3'-carboxamide} for radiolabeling was synthesized from 2-bromopyridin-3-ol and 5″-chloro-[2,2':5',3″-terpyridine]-3'-carboxylic acid in 6 steps with 17% overall chemical yield. The target tracer [(11)C]MK-1064 {5″-chloro-N-((5-[(11)C]methoxy-6-methoxypyridin-2-yl)methyl)-[2,2':5',3″-terpyridine]-3'-carboxamide} was prepared by O-[(11)C]methylation of its corresponding precursor desmethyl-MK-1064 with [(11)C]CH3OTf under basic condition and isolated by a simplified solid-phase extraction (SPE) method in 50-60% decay corrected radiochemical yields based on [(11)C]CO2 at end of bombardment (EOB). The overall synthesis time from EOB was 23min, the radiochemical purity was >99%, and the specific activity at end of synthesis (EOS) was 185-555GBq/μmol. PMID:27268698

  4. ?Linear Gas Jet with Tailored Density Profile"

    SciTech Connect

    KRISHNAN, Mahadevan

    2012-12-10

    Supersonic, highly collimated gas jets and gas-filled capillary discharge waveguides are two primary targets of choice for Laser Plasma Accelerators (LPA) . Present gas jets have lengths of only 2-4 mm at densities of 1-4E19 cm-3, sufficient for self trapping and electron acceleration to energies up to ~150 MeV. Capillary structures 3 cm long have been used to accelerate beams up to 1 GeV. Capillary discharges used in LPAs serve to guide the pump laser and optimize the energy gain. A wall-stabilized capillary discharge provides a transverse profile across the channel that helps guide the laser and combat diffraction. Gas injection via a fast nozzle at one end provides some longitudinal density control, to improve the coupling. Gas jets with uniform or controlled density profiles may be used to control electron bunch injection and are being integrated into capillary experiments to add tuning of density. The gas jet for electron injection has not yet been optimized. Our Ph-I results have provided the LPA community with an alternative path to realizing a 2-3GeV electron bunch using just a gas jet. For example, our slit/blade combination gives a 15-20mm long acceleration path with tunable density profile, serving as an alternative to a 20-mm long capillary discharge with gas injection at one end. In Ph-II, we will extend these results to longer nozzles, to see whether we can synthesize 30 or 40-mm long plasma channels for LPAs.

  5. On magnetohydrodynamic solitons in jets

    NASA Technical Reports Server (NTRS)

    Roberts, B.

    1987-01-01

    Nonlinear solitary wave propagation in a compressible magnetic beam model of an extragalactic radio jet is examined and shown to lead to solitons of the Benjamin-Ono type. A number of similarities between such magnetic beam models of jets and models of solar photospheric flux tubes are pointed out and exploited. A single soliton has the appearance of a symmetric bulge on the jet which propagates faster than the jet's flow.

  6. Multiple Plasma Ejections and Intermittent Nature of Magnetic Reconnection in Solar Chromospheric Anemone Jets

    NASA Astrophysics Data System (ADS)

    Singh, K. A. P.; Isobe, H.; Nishizuka, N.; Nishida, K.; Shibata, K.

    2012-11-01

    The recent discovery of chromospheric anemone jets with the Solar Optical Telescope (SOT) on board Hinode has shown an indirect evidence of magnetic reconnection in the solar chromosphere. However, the basic nature of magnetic reconnection in chromosphere is still unclear. We studied nine chromospheric anemone jets from SOT/Hinode using Ca II H filtergrams, and we found multiple bright, plasma ejections along the jets. In most cases, the major intensity enhancements (larger than 30% relative to the background intensity) of the loop correspond to the timing of the plasma ejections. The typical lifetime and size of the plasma ejecta are about 20-60 s and 0.3-1.5 Mm, respectively. The height-time plot of jet shows many sub-structures (or individual jets) and the typical lifetime of the individual jet is about one to five minutes. Before the onset of the jet activity, a loop appears in Ca II H and gradually increases in size, and after few minutes several jets are launched from the loop. Once the jet activity starts and several individual jets are launched, the loop starts shrinking with a speed of ~4 km s-1. In some events, a downward moving blob with a speed of ~35 km s-1 was observed, associated with the upward moving plasma along one of the legs of the loop hosting the jets. The upward moving plasma gradually developed into jets. Multiple plasma ejections in chromospheric anemone jet show the strongly time-dependent as well as intermittent nature of magnetic reconnection in the solar chromosphere.

  7. MULTIPLE PLASMA EJECTIONS AND INTERMITTENT NATURE OF MAGNETIC RECONNECTION IN SOLAR CHROMOSPHERIC ANEMONE JETS

    SciTech Connect

    Singh, K. A. P.; Nishida, K.; Shibata, K.; Isobe, H.; Nishizuka, N. E-mail: nishida@kwasan.kyoto-u.ac.jp E-mail: isobe@kwasan.kyoto-u.ac.jp

    2012-11-01

    The recent discovery of chromospheric anemone jets with the Solar Optical Telescope (SOT) on board Hinode has shown an indirect evidence of magnetic reconnection in the solar chromosphere. However, the basic nature of magnetic reconnection in chromosphere is still unclear. We studied nine chromospheric anemone jets from SOT/Hinode using Ca II H filtergrams, and we found multiple bright, plasma ejections along the jets. In most cases, the major intensity enhancements (larger than 30% relative to the background intensity) of the loop correspond to the timing of the plasma ejections. The typical lifetime and size of the plasma ejecta are about 20-60 s and 0.3-1.5 Mm, respectively. The height-time plot of jet shows many sub-structures (or individual jets) and the typical lifetime of the individual jet is about one to five minutes. Before the onset of the jet activity, a loop appears in Ca II H and gradually increases in size, and after few minutes several jets are launched from the loop. Once the jet activity starts and several individual jets are launched, the loop starts shrinking with a speed of {approx}4 km s{sup -1}. In some events, a downward moving blob with a speed of {approx}35 km s{sup -1} was observed, associated with the upward moving plasma along one of the legs of the loop hosting the jets. The upward moving plasma gradually developed into jets. Multiple plasma ejections in chromospheric anemone jet show the strongly time-dependent as well as intermittent nature of magnetic reconnection in the solar chromosphere.

  8. High pt jets and photons at the Tevatron

    SciTech Connect

    Gerber, Cecilia E.; /Illinois U., Chicago

    2005-09-01

    The authors present recent results on high p{sub T} jets and photon production in {bar p}p collisions at a center of mass energy of 1.96 TeV. The measurements were performed by the CDF and D0 collaborations using between 150 and 300 pb{sup -1} of data taken during Run II at the Tevatron.

  9. Multiwavelength study of 20 jets that emanate from the periphery of active regions

    NASA Astrophysics Data System (ADS)

    Mulay, Sargam M.; Tripathi, Durgesh; Del Zanna, Giulio; Mason, Helen

    2016-05-01

    T [K] = 6.2/6.3 (~2 MK). In addition, we derived an emission measure and a lower limit of electron density at the location of the spire (jet 1: log EM = 28.6, Ne = 1.3 × 1010 cm-3; jet 2: log EM = 28.0, Ne = 8.6 × 109 cm-3) and the footpoint (jet 1 - log EM = 28.6, Ne = 1.1 × 1010 cm-3; jet 2: log EM = 28.1, Ne = 8.4 × 109 cm-3). These results are in agreement with those obtained earlier by studying individual active region jets. Conclusions: The observation of flux cancellation, the association with HXR emission and emission of nonthermal type-III radio bursts, suggest that the initiation and therefore, heating is taking place at the base of the jet. This is also supported by the high temperature plasma revealed by the DEM analysis in the jet footpoint (peak in the DEM at log T [K] = 6.5). Our results provide substantial constraints for theoretical modeling of the jets and their thermodynamic nature. Movies are available in electronic form at http://www.aanda.org

  10. Multiwavelength study of 20 jets that emanate from the periphery of active regions

    NASA Astrophysics Data System (ADS)

    Mulay, Sargam M.; Tripathi, Durgesh; Del Zanna, Giulio; Mason, Helen

    2016-04-01

    T [K] = 6.2/6.3 (~2 MK). In addition, we derived an emission measure and a lower limit of electron density at the location of the spire (jet 1: log EM = 28.6, Ne = 1.3 × 1010 cm-3; jet 2: log EM = 28.0, Ne = 8.6 × 109 cm-3) and the footpoint (jet 1 - log EM = 28.6, Ne = 1.1 × 1010 cm-3; jet 2: log EM = 28.1, Ne = 8.4 × 109 cm-3). These results are in agreement with those obtained earlier by studying individual active region jets. Conclusions: The observation of flux cancellation, the association with HXR emission and emission of nonthermal type-III radio bursts, suggest that the initiation and therefore, heating is taking place at the base of the jet. This is also supported by the high temperature plasma revealed by the DEM analysis in the jet footpoint (peak in the DEM at log T [K] = 6.5). Our results provide substantial constraints for theoretical modeling of the jets and their thermodynamic nature. Movies are available in electronic form at http://www.aanda.org

  11. Air admixture to exhaust jets

    NASA Technical Reports Server (NTRS)

    Sanger, Eugen

    1953-01-01

    The problem of thrust increase by air admixture to exhaust jets of rockets, turbojet, ram- and pulse-jet engines is investigated theoretically. The optimum ratio of mixing chamber pressure to ambient pressure and speed range for thrust increase due to air admixture is determined for each type of jet engine.

  12. Inhibition of phoshodiesterase type 2 or type 10 reverses object memory deficits induced by scopolamine or MK-801.

    PubMed

    Reneerkens, Olga A H; Rutten, Kris; Bollen, Eva; Hage, Thorsten; Blokland, Arjan; Steinbusch, Harry W M; Prickaerts, Jos

    2013-01-01

    The objective of this study was to assess the effects of phosphodiesterase type 2 (PDE2) and type 10 (PDE10) inhibition on memory function in the object recognition task using the scopolamine- and MK-801-induced memory deficit model. The effects of the PDE2 inhibitor BAY 60-7550 and the PDE10 inhibitor PQ-10 on object recognition performance were investigated in the scopolamine (0.1mg/kg, i.p.) or MK-801 (0.125 mg/kg, i.p.) model. BAY 60-7550 was tested at a dose of 0.3-3mg/kg (p.o.) in both models; PQ-10 was tested at doses of 0.1-1mg/kg (p.o.) in the scopolamine model and 0.3-3mg/kg in the MK-801 model. All compounds were injected 30 min before the learning trial. Both BAY 60-7550 (1mg/kg) and PQ-10 (0.3mg/kg) attenuated the scopolamine-induced memory deficit. The MK-801-induced memory deficit was reversed after treatment with each PDE inhibitor at a dose of 1mg/kg or higher. PQ10 was highly brain penetrant, whereas 60-7550 levels in the brain were very low after oral treatment. We concluded that since BAY 60-7550 and PQ10 reversed both scopolamine- and MK-801-induced memory deficits, this supports the notion that dual substrate PDE inhibitors might be suitable candidates for cognition enhancement. PMID:22951181

  13. Knockout of NMDA-receptors from parvalbumin interneurons sensitizes to schizophrenia-related deficits induced by MK-801.

    PubMed

    Bygrave, A M; Masiulis, S; Nicholson, E; Berkemann, M; Barkus, C; Sprengel, R; Harrison, P J; Kullmann, D M; Bannerman, D M; Kätzel, D

    2016-01-01

    It has been suggested that a functional deficit in NMDA-receptors (NMDARs) on parvalbumin (PV)-positive interneurons (PV-NMDARs) is central to the pathophysiology of schizophrenia. Supportive evidence come from examination of genetically modified mice where the obligatory NMDAR-subunit GluN1 (also known as NR1) has been deleted from PV interneurons by Cre-mediated knockout of the corresponding gene Grin1 (Grin1(ΔPV) mice). Notably, such PV-specific GluN1 ablation has been reported to blunt the induction of hyperlocomotion (a surrogate for psychosis) by pharmacological NMDAR blockade with the non-competitive antagonist MK-801. This suggests PV-NMDARs as the site of the psychosis-inducing action of MK-801. In contrast to this hypothesis, we show here that Grin1(ΔPV) mice are not protected against the effects of MK-801, but are in fact sensitized to many of them. Compared with control animals, Grin1(ΔPV)mice injected with MK-801 show increased stereotypy and pronounced catalepsy, which confound the locomotor readout. Furthermore, in Grin1(ΔPV)mice, MK-801 induced medial-prefrontal delta (4 Hz) oscillations, and impaired performance on tests of motor coordination, working memory and sucrose preference, even at lower doses than in wild-type controls. We also found that untreated Grin1(ΔPV)mice are largely normal across a wide range of cognitive functions, including attention, cognitive flexibility and various forms of short-term memory. Taken together these results argue against PV-specific NMDAR hypofunction as a key starting point of schizophrenia pathophysiology, but support a model where NMDAR hypofunction in multiple cell types contribute to the disease. PMID:27070406

  14. Knockout of NMDA-receptors from parvalbumin interneurons sensitizes to schizophrenia-related deficits induced by MK-801

    PubMed Central

    Bygrave, A M; Masiulis, S; Nicholson, E; Berkemann, M; Barkus, C; Sprengel, R; Harrison, P J; Kullmann, D M; Bannerman, D M; Kätzel, D

    2016-01-01

    It has been suggested that a functional deficit in NMDA-receptors (NMDARs) on parvalbumin (PV)-positive interneurons (PV-NMDARs) is central to the pathophysiology of schizophrenia. Supportive evidence come from examination of genetically modified mice where the obligatory NMDAR-subunit GluN1 (also known as NR1) has been deleted from PV interneurons by Cre-mediated knockout of the corresponding gene Grin1 (Grin1ΔPV mice). Notably, such PV-specific GluN1 ablation has been reported to blunt the induction of hyperlocomotion (a surrogate for psychosis) by pharmacological NMDAR blockade with the non-competitive antagonist MK-801. This suggests PV-NMDARs as the site of the psychosis-inducing action of MK-801. In contrast to this hypothesis, we show here that Grin1ΔPV mice are not protected against the effects of MK-801, but are in fact sensitized to many of them. Compared with control animals, Grin1ΔPVmice injected with MK-801 show increased stereotypy and pronounced catalepsy, which confound the locomotor readout. Furthermore, in Grin1ΔPVmice, MK-801 induced medial-prefrontal delta (4 Hz) oscillations, and impaired performance on tests of motor coordination, working memory and sucrose preference, even at lower doses than in wild-type controls. We also found that untreated Grin1ΔPVmice are largely normal across a wide range of cognitive functions, including attention, cognitive flexibility and various forms of short-term memory. Taken together these results argue against PV-specific NMDAR hypofunction as a key starting point of schizophrenia pathophysiology, but support a model where NMDAR hypofunction in multiple cell types contribute to the disease. PMID:27070406

  15. Surface pressure fluctuations due to an impinging underexpanded supersonic jet

    NASA Astrophysics Data System (ADS)

    Pundir, Binu

    The impingement of supersonic jets on surfaces is of interest because of its important application to jet blast deflectors (JBD), and short takeoff and vertical landing aircraft (STOVL) during hover. Typically, on an aircraft carrier deck, the impingement of the jet blast on the deflector generates impingement tones, and structural vibrations, not only on the JBD but also on the ship deck. Therefore, apart from direct transmission of jet noise to the gallery level, there is a component of noise transmitted due to the impingement of the jet on the JBD. The objectives of this work are to study the pressure spectra (i) on a flat plate, and separately on a cone due to axisymmetric impingement of a supersonic underexpanded cold jet issuing from a convergent-divergent nozzle and (ii) on a plane jet impinging on a finite plate and an adjoining ground plane due to the impingement of a planar jet on the plate. The characteristics of the surface pressure fluctuations are numerically investigated using WIND-US 2.0. The time-dependent, compressible Euler equations for perfect gas are employed for the present computations. The impingement distance between the jet nozzle and the deflector plate, and the plate inclination with respect to the incident jet are varied. The impingement zone stagnation bubble and a high-speed radial jet with several embedded structures (shocklets) were identified on the perpendicular plate. Flows involving cones reveal the presence of detached cone shocks, enclosing a recirculation zone. The location and magnitude of the peak pressure on the cone surface are a strong function of the cone apex angle. For the two-dimensional jet impingement on angled plate the peak value of pressure occurs at normal jet impingement. The pressure at the intersection point of the plate and the ground plane is sometimes higher than the peak pressure on the plate. Beyond this point there is a sharp decrease in pressure. As the flow accelerates, an oblique shock is sometimes

  16. Relativistic HD and MHD modelling for AGN jets

    NASA Astrophysics Data System (ADS)

    Keppens, R.; Porth, O.; Monceau-Baroux, R.; Walg, S.

    2013-12-01

    Relativistic hydro and magnetohydrodynamics (MHD) provide a continuum fluid description for plasma dynamics characterized by shock-dominated flows approaching the speed of light. Significant progress in its numerical modelling emerged in the last two decades; we highlight selected examples of modern grid-adaptive, massively parallel simulations realized by our open-source software MPI-AMRVAC (Keppens et al 2012 J. Comput. Phys. 231 718). Hydrodynamical models quantify how energy transfer from active galactic nuclei (AGN) jets to their surrounding interstellar/intergalactic medium (ISM/IGM) gets mediated through shocks and various fluid instability mechanisms (Monceau-Baroux et al 2012 Astron. Astrophys. 545 A62). With jet parameters representative for Fanaroff-Riley type-II jets with finite opening angles, we can quantify the ISM volumes affected by jet injection and distinguish the roles of mixing versus shock-heating in cocoon regions. This provides insight in energy feedback by AGN jets, usually incorporated parametrically in cosmological evolution scenarios. We discuss recent axisymmetric studies up to full 3D simulations for precessing relativistic jets, where synthetic radio maps can confront observations. While relativistic hydrodynamic models allow one to better constrain dynamical parameters like the Lorentz factor and density contrast between jets and their surroundings, the role of magnetic fields in AGN jet dynamics and propagation characteristics needs full relativistic MHD treatments. Then, we can demonstrate the collimating properties of an overal helical magnetic field backbone and study differences between poloidal versus toroidal field dominated scenarios (Keppens et al 2008 Astron. Astrophys. 486 663). Full 3D simulations allow one to consider the fate of non-axisymmetric perturbations on relativistic jet propagation from rotating magnetospheres (Porth 2013 Mon. Not. R. Astron. Soc. 429 2482). Self-stabilization mechanisms related to the detailed

  17. Comparison of gaseous exhaust indices of the F109 turbofan using three different blends of petroleum-based Jet-A and camelina-based Jet-A

    NASA Astrophysics Data System (ADS)

    Kozak, Brian John

    This research project focused on the collection and comparison of gaseous exhaust emissions of the F109 turbofan engine using petroleum-based Jet-A and two different blends of camelina-based Jet-A. Simulated landing and takeoff cycles were used to collect gaseous exhaust emissions. Unburned hydrocarbon (HC), nitrogen oxide (NOx), and carbon moNOxide (CO) exhaust indices (EIm) were calculated using ICAO Annex 16 Volume II formulae. Statistical analyses were performed on the Elm data. There was no significant difference in HC EIm and CO EI m among the three fuels at takeoff thrust. There were significant differences among the fuels for NOx EIm. 50% Jet-A 50% camelina produced the highest NOx EIm, then 75% Jet-A 25% camelina and finally Jet-A. At climb thrust, both blends of camelina fuel produced higher NOx EIm but no difference in CO EIm and HC EIm as Jet-A. At approach thrust, both blends of camelina fuel produced higher NOx EIm, lower CO EIm, and no difference in HC EIm as Jet-A. At idle thrust, there was no significant difference among the fuels for NOx EIm. There were significant differences among the fuels for HC EIm. Jet-A and 50% Jet-A 50% both produced higher HC EIm as 75% Jet-A 25% camelina. There were significant differences among the fuels for CO EI m. Jet-A produced the highest CO EIm, then 75% Jet-A 25% camelina and finally 50% Jet-A 50% camelina.

  18. The physics of jets

    SciTech Connect

    Hofmann, W.

    1987-09-01

    Recent data on the fragmentation of quarks and gluons is discussed in the context of phenomenological models of parton fragmentation. Emphasis is placed on the experimental evidence for parton showers as compared to a fixed order QCD treatment, on new data on inclusive hadron production and on detailed studies of baryon production in jets.

  19. Particle Acceleration in Jets

    NASA Technical Reports Server (NTRS)

    Nishikawa, Ken-Ichi

    2005-01-01

    Nonthermal radiation observed from astrophysical systems containing relativistic jets and shocks, e.g., active galactic nuclei (AGNs), gamma ray burst (GRBs), and Galactic microquasar systems usually have power-law emission spectra. Fermi acceleration is the mechanism usually assumed for the acceleration of particles in astrophysical environments.

  20. Jet Screech Noise Computation

    NASA Technical Reports Server (NTRS)

    Loh, Ching Y.; Hultgren, Lennart S.

    2003-01-01

    The near-field screech-tone noise of a typical underexpanded circular jet issuing from a sonic nozzle is simulated numerically. The self-sustained feedback loop is automatically established in the simulation. The computed shock-cell structure, acoustic wave length, screech tone frequencies, and sound pressure levels in the near field are in good agreement with existing experimental results.

  1. Vortex diode jet

    DOEpatents

    Houck, Edward D.

    1994-01-01

    A fluid transfer system that combines a vortex diode with a jet ejector to transfer liquid from one tank to a second tank by a gas pressurization method having no moving mechanical parts in the fluid system. The vortex diode is a device that has a high resistance to flow in one direction and a low resistance to flow in the other.

  2. Spectroscopy with Supersonic Jets.

    ERIC Educational Resources Information Center

    Skinner, Anne R.; Chandler, Dean W.

    1980-01-01

    Discusses a new technique that enables spectroscopists to study gas phase molecules at temperatures below 1 K, without traditional cryogenic apparatus. This technique uses supersonic jets as samples for gas molecular spectroscopy. Highlighted are points in the theory of supersonic flow which are important for applications in molecular…

  3. The Jet Travel Challenge

    ERIC Educational Resources Information Center

    Roman, Harry T.

    2007-01-01

    Airplane travelers are dismayed by the long lines and seemingly chaotic activities that precede boarding a full airplane. Surely, the one who can solve this problem is going to make many travelers happy. This article describes the Jet Travel Challenge, an activity that challenges students to create some alternatives to this now frustrating…

  4. Tracing Massive Protostellar Jets from Intermediate-Mass Protostars in the Carina Nebula

    NASA Astrophysics Data System (ADS)

    Reiter, A.

    2014-09-01

    We present new spectroscopy and imaging of four protostellar jets in the Carina nebula. Near-IR [Fe II] emission traces dense gas in the jet that is self-shielded from Lyman continuum photons from nearby O-type stars. New near-IR [Fe II] images reveal a substantial mass of dense, neutral gas that is not seen in the Halpha emission from these jets, leading to densities and mass-loss rate estimates an order of magnitude larger than those derived from the Halpha emission measure. Higher jet mass-loss rates require higher accretion rates, implying that these jets are driven by intermediate-mass (around 2 - 8 solar masses) protostars. Velocities from new proper motion and spectroscopic measurements fall among the velocities typically measured in lower-luminosity sources (100 - 200 km/s). We propose that these jets reflect essentially the same outflow phenomenon seen in low-mass protostars, but that the collimated atomic jet core is irradiated and rendered observable. Thus, the jets in Carina constitute a new view of collimated jets from intermediate-mass protostars that exist in a feedback-dominated environment, and offer strong additional evidence that stars up to 8 solar masses form by the same accretion mechanisms as low-mass stars.

  5. The combination of glutamate receptor antagonist MK-801 with tamoxifen and its active metabolites potentiates their antiproliferative activity in mouse melanoma K1735-M2 cells

    SciTech Connect

    Ribeiro, Mariana P.C.; Nunes-Correia, Isabel; Santos, Armanda E.; Custódio, José B.A.

    2014-02-15

    Recent reports suggest that N-methyl-D-aspartate receptor (NMDAR) blockade by MK-801 decreases tumor growth. Thus, we investigated whether other ionotropic glutamate receptor (iGluR) antagonists were also able to modulate the proliferation of melanoma cells. On the other hand, the antiestrogen tamoxifen (TAM) decreases the proliferation of melanoma cells, and is included in combined therapies for melanoma. As the efficacy of TAM is limited by its metabolism, we investigated the effects of the NMDAR antagonist MK-801 in combination with TAM and its active metabolites, 4-hydroxytamoxifen (OHTAM) and endoxifen (EDX). The NMDAR blockers MK-801 and memantine decreased mouse melanoma K1735-M2 cell proliferation. In contrast, the NMDAR competitive antagonist APV and the AMPA and kainate receptor antagonist NBQX did not affect cell proliferation, suggesting that among the iGluR antagonists only the NMDAR channel blockers inhibit melanoma cell proliferation. The combination of antiestrogens with MK-801 potentiated their individual effects on cell biomass due to diminished cell proliferation, since it decreased the cell number and DNA synthesis without increasing cell death. Importantly, TAM metabolites combined with MK-801 promoted cell cycle arrest in G1. Therefore, the data obtained suggest that the activity of MK-801 and antiestrogens in K1735-M2 cells is greatly enhanced when used in combination. - Highlights: • MK-801 and memantine decrease melanoma cell proliferation. • The combination of MK-801 with antiestrogens inhibits melanoma cell proliferation. • These combinations greatly enhance the effects of the compounds individually. • MK-801 combined with tamoxifen active metabolites induces cell cycle arrest in G1. • The combination of MK-801 and antiestrogens is an innovative strategy for melanoma.

  6. Purification and Low Temperature (15 MK-10 k) NMR of Ultra Pure Ortho-Deuterium

    NASA Astrophysics Data System (ADS)

    Yucel, Sermet

    1988-06-01

    Pure solid ortho-deuterium, whose spin-lattice relaxation time in the liquid helium region is expected to be the order of days, is a promising candidate for nuclear spin polarized fusion fuels and targets for high energy physics applications. For purification of ortho-deuterium by conversion of para-deuterium, we present here a new catalytic technique in which conversion takes place in the physisorbed phase on copper powder or on grafoil at temperatures between 12 and 8 K. This catalysis has yielded an ortho-D_2 purity in which para -D_2 concentration is reduced to 1.1 times 10^{ -3}, better by more than an order of magnitude than in any previous work. By subsequent aging of the purified samples at 4.2 K for about 9 months, o-D _2 with residual p-D_2 concentration of 5 +/- 2 times 10^{-5} was attained, whose spin-lattice relaxation time at 1.2 K in a 0.3 T magnetic field is 11 hours. A theory of ortho -para conversion on clean metal surfaces due to interaction with conduction electrons is given. In the case of grafoil, the conversion rates in 2-D solid phases of physisorbed D_2 by emission of one and two photons (of the D_2 solid) are calculated. A temperature independent conversion rate is predicted and is consistent with our measurements in the 2-D solid regime. A sample of moderately pure o-D_2, (p-D_2 concentration of 0.004), chosen for acceptable heat of conversion as well as relatively short spin-lattice relaxation time even in the mK region, was cooled in our dilution refrigerator to 15 mK, a record low temperature for solid hydrogens. The special NMR probe and sample chamber designed and constructed for this purpose is described. With our present apparatus (magnetic field of 13 Tesla), protein polarizations of about 70% are attainable, from which deuteron polarizations in excess of 50% can be driven by dynamic polarization techniques in an o-D _2-HD mixture.

  7. TREAT MK III Loop Thermoelastoplastic Stress Analysis for the L03 Experiment

    SciTech Connect

    Kennedy, James M.

    1981-03-01

    The STRAW code was used to analyze the static response of a TREAT MK III loop subjected to thermal and mechanical loadings arising from an accident situation for the purpose of determining the defiections and stresses. This analysis provides safety support for the L03 reactivity accident study. The analysis was subdivided into two tasks: (1) an analysis of a flow blockage accident (Cases A and B), where all the energy is assumed deposited in the test leg, resulting in a temperature increase from 530°F to 1720°F, with a small internal pressure throughout the loop and (2) an analysis of a second flow blockage accident (Cases C and D), where again, all the energy is assumed to he deposited in the test leg, resulting in a temperature rise from 530°F to 1845°F, with a small internal pressure throughout the loop. The purpose of these two tasks was to determine if loop failure can occur with the thermal differential across the pump and test legs. Also of interest is whether an undesirable amount of loop lateral deflection will be caused by the thermal differential. A two dimensional analysis of the TREAT MK III loop was performed. The analysis accounted for material nonlinearities, both as a function of temperature and stress, and geometric nonlinearities arising from large deflections. Straight beam elements with annular cross sections were used to model the loop. The analyses show that the maximum strains are less than 21% of their failure strains for all subcases of Cases A and B. For all subcases of cases C and D, the maximum strains are less than 53% of their failure strains. The failure strain is 27.9% for the material at 530°F, 38.1% at 1720°F and 17.8% at 1845°F. Large lateral deflections are observed when the loop is not constrained except at its clamped support--as much as 8.6 inches. However, by accounting for the constraint of the concrete biological shield, the maximum lateral deflection was reduced to less than 0.05 inches at the points of concern.

  8. Dynamics of relativistic jets

    NASA Astrophysics Data System (ADS)

    Nishikawa, K.-I.; Frank, J.; Christodoulou, D. M.; Koide, S.; Sakai, J.-I.; Sol, Hélène; Mutel, Robert L.

    1998-12-01

    We discuss the structure and relativistic kinematics that develop in three spatial dimensions when a moderately hot, supersonic jet propagates into a denser background medium and encounters resistance from an oblique magnetic field. Our simulations incorporate relativistic MHD in a four-dimensional spacetime and clearly show that (a) relatively weak, oblique fields (at 1/16 of the equipartition value) have only a negligible influence on the propagating jet and they are passively pushed away by the relativistically moving head; (b) oblique fields in equipartition with the ambient plasma provide more resistance and cause bending at the jet head, but the magnitude of this deflection and the associated backflow are small compared to those identified by previous studies. The new results are understood as follows: Relativistic simulations have consistently shown that these jets are effectively heavy and so they do not suffer substantial momentum losses and are not decelerated as efficiently as their nonrelativistic counterparts. In addition, the ambient magnetic field, however strong, can be pushed aside with relative ease by the beam, provided that the degrees of freedom associated with all three spatial dimensions are followed self-consistently during the simulations. The effect is analogous to pushing Japanese "noren" or vertical Venetian blinds out of the way while the slats are allowed to bend and twist in 3-D space. Applied to relativistic extragalactic jets from blazars, the new results are encouraging since superluminal outflows exhibit bending near their sources and their environments are profoundly magnetized - but observations do not provide support for irregular kinematics such as large-scale vortical motions and pronounced reverse flows near the points of origin.

  9. Jet propagation through energetic materials

    SciTech Connect

    Pincosy, P; Poulsen, P

    2004-01-08

    In applications where jets propagate through energetic materials, they have been observed to become sufficiently perturbed to reduce their ability to effectively penetrate subsequent material. Analytical calculations of the jet Bernoulli flow provides an estimate of the onset and extent of such perturbations. Although two-dimensional calculations show the back-flow interaction pressure pulses, the symmetry dictates that the flow remains axial. In three dimensions the same pressure impulses can be asymmetrical if the jet is asymmetrical. The 3D calculations thus show parts of the jet having a significant component of radial velocity. On the average the downstream effects of this radial flow can be estimated and calculated by a 2D code by applying a symmetrical radial component to the jet at the appropriate position as the jet propagates through the energetic material. We have calculated the 3D propagation of a radio graphed TOW2 jet with measured variations in straightness and diameter. The resultant three-dimensional perturbations on the jet result in radial flow, which eventually tears apart the coherent jet flow. This calculated jet is compared with jet radiographs after passage through the energetic material for various material thickness and plate thicknesses. We noted that confinement due to a bounding metal plate on the energetic material extends the pressure duration and extent of the perturbation.

  10. Radiant Energy Power Source for Jet Aircraft

    SciTech Connect

    Doellner, O.L.

    1992-02-01

    This report beings with a historical overview on the origin and early beginnings of Radiant Energy Power Source for Jet Aircraft. The report reviews the work done in Phase I (Grant DE-FG01-82CE-15144) and then gives a discussion of Phase II (Grant DE-FG01-86CE-15301). Included is a reasonably detailed discussion of photovoltaic cells and the research and development needed in this area. The report closes with a historical perspective and summary related to situations historically encountered on projects of this nature. 15 refs.

  11. Searches in photon and jet states

    SciTech Connect

    Soha, A.; /UC, Davis

    2007-06-01

    The authors present recent results from the Collider Detector at Fermilab (CDF) and D0 experiments using data from proton-antiproton collisions with {radical}s = 1.96 TeV at Run II of the Fermilab Tevatron. New physics may appear in events with high transverse momentum objects, including photons and quark or gluon jets. The results described here are of signature-based searches and model-based searches probing supersymmetry, leptoquarks, 4th generation quarks, and large extra dimensions.

  12. Acoustic characteristics of twin jets.

    PubMed

    He, F; Zhang, X W

    2002-09-01

    Experiments were conducted to investigate the acoustic characteristics of underexpanded supersonic twin jets in different azimuthal measurement planes. Compared with two independent jets, the twin jets produced additional noise due to the enhanced mixing and entrainment. The larger pressure ratio for switching from the axisymmetric mode to the helical mode led to lower noise levels at 90 degrees than for two independent jets. For pressure ratios greater than 5.00, the noise reduction was due to cessation of screeching of the twin jets while screeching of a single jet was still detected. The apparent shielding phenomenon was measured for the screech helical mode. The screech tone intensities were attenuated largely due to the shielding effects. The noise reductions due to shielding were obtained over a wide range of pressure ratios relative to the sum of two independent jets. PMID:12243185

  13. Pileup subtraction for jet shapes.

    PubMed

    Soyez, Gregory; Salam, Gavin P; Kim, Ji-Hun; Dutta, Souvik; Cacciari, Matteo

    2013-04-19

    Jets in high energy hadronic collisions often contain the fingerprints of the particles that produced them. Those fingerprints, and thus the nature of the particles that produced the jets, can be read off with the help of quantities known as jet shapes. Jet shapes are, however, severely affected by pileup, the accumulation in the detector of the residues of the many simultaneous collisions taking place in the Large Hadron Collider (LHC). We introduce a method to correct for pileup effects in jet shapes. Relative to earlier, limited approaches, the key advance resides in its full generality, achieved through a numerical determination, for each jet, of a given shape's susceptibility to pileup. The method rescues the possibility of using jet shapes in the high pileup environment of current and future LHC running, as we show with examples of quark-gluon discrimination and top tagging. PMID:23679594

  14. Flow cytometer jet monitor system

    DOEpatents

    Van den Engh, Ger

    1997-01-01

    A direct jet monitor illuminates the jet of a flow cytometer in a monitor wavelength band which is substantially separate from the substance wavelength band. When a laser is used to cause fluorescence of the substance, it may be appropriate to use an infrared source to illuminate the jet and thus optically monitor the conditions within the jet through a CCD camera or the like. This optical monitoring may be provided to some type of controller or feedback system which automatically changes either the horizontal location of the jet, the point at which droplet separation occurs, or some other condition within the jet in order to maintain optimum conditions. The direct jet monitor may be operated simultaneously with the substance property sensing and analysis system so that continuous monitoring may be achieved without interfering with the substance data gathering and may be configured so as to allow the front of the analysis or free fall area to be unobstructed during processing.

  15. Position-Dependent Optical Response of a Superconducting Resonator at 15 mK

    NASA Astrophysics Data System (ADS)

    Voigt, K. D.; Hertzberg, J. B.; Hoffman, J. E.; Grover, J. A.; Lee, J.; Solano, P.; Budoyo, R. P.; Ballard, C.; Anderson, J. R.; Lobb, C. J.; Orozco, L. A.; Rolston, S. L.; Wellstood, F. C.

    2015-03-01

    We have studied the optical and dielectric response of a translatable thin-film lumped-element superconducting Al microwave resonator cooled to 15 mK. The resonator has a resonance frequency of 6.14 GHz, a quality factor Q of 2.59 x 105and is mounted inside a superconducting aluminum 3D cavity. A tapered optical fiber enters and exits the 3D cavity through two small holes in opposite sides of the cavity, placed so that the fiber can pass close to the resonator. The 3D cavity is mounted on an x-z piezo-translation stage that allows us to change the relative position of the lumped-element resonator and fiber. When the resonator is brought near to the fiber, we observe a shift in resonance frequency due to the presence of the fiber dielectric. When light is sent through the fiber, Rayleigh scattering causes a position-dependent illumination of the resonator, generating quasiparticles and thereby affecting its resonance frequency and Q. Our model of the resonator response includes the generation, diffusion, and recombination of quasiparticles in the resonator and shows that the frequency response allows us to track the position of the fiber in situ. Work supported by NSF through the Physics Frontier Center at the Joint Quantum Institute, Dept. of Physics, Univ. of Maryland.

  16. Verification of JUPITER Standard Analysis Method for Upgrading Joyo MK-III Core Design and Management

    NASA Astrophysics Data System (ADS)

    Maeda, Shigetaka; Ito, Chikara; Sekine, Takashi; Aoyama, Takafumi

    In the experimental fast reactor Joyo, loading of irradiation test rigs causes a decrease in excess reactivity because the rigs contain less fissile materials than the driver fuel. In order to carry out duty operation cycles using as many irradiation rigs as possible, it is necessary to upgrade the core performance to increase its excess reactivity and irradiation capacity. Core modification plans have been considered, such as the installation of advanced radial reflectors and reduction of the number of control rods. To implement such core modifications, it is first necessary to improve the prediction accuracy in core design and to optimize safety margins. In the present study, verification of the JUPITER fast reactor standard analysis method was conducted through a comparison between the calculated and the measured Joyo MK-III core characteristics, and it was concluded that the accuracy for a small sodium-cooled fast reactor with a hard neutron spectrum was within 5 % of unity. It was shown that, the performance of the irradiation bed core could be upgraded by the improvement of the prediction accuracy of the core characteristics and optimization of safety margins.

  17. Invited review article: A 10 mK scanning probe microscopy facility.

    PubMed

    Song, Young Jae; Otte, Alexander F; Shvarts, Vladimir; Zhao, Zuyu; Kuk, Young; Blankenship, Steven R; Band, Alan; Hess, Frank M; Stroscio, Joseph A

    2010-12-01

    We describe the design, development and performance of a scanning probe microscopy (SPM) facility operating at a base temperature of 10 mK in magnetic fields up to 15 T. The microscope is cooled by a custom designed, fully ultra-high vacuum (UHV) compatible dilution refrigerator (DR) and is capable of in situ tip and sample exchange. Subpicometer stability at the tip-sample junction is achieved through three independent vibration isolation stages and careful design of the dilution refrigerator. The system can be connected to, or disconnected from, a network of interconnected auxiliary UHV chambers, which include growth chambers for metal and semiconductor samples, a field-ion microscope for tip characterization, and a fully independent additional quick access low temperature scanning tunneling microscope (STM) and atomic force microscope (AFM) system. To characterize the system, we present the cooling performance of the DR, vibrational, tunneling current, and tip-sample displacement noise measurements. In addition, we show the spectral resolution capabilities with tunneling spectroscopy results obtained on an epitaxial graphene sample resolving the quantum Landau levels in a magnetic field, including the sublevels corresponding to the lifting of the electron spin and valley degeneracies. PMID:21198007

  18. STM study of the superconducting proximity effect at 60 mK: influence of interface transparency.

    NASA Astrophysics Data System (ADS)

    Courtois, H.; Gupta, A. K.; Cretinon, L.; Moussy, N.; Pannetier, B.

    2002-03-01

    Proximity effect of a superconductor on a normal metal has a renewed interest recently. The effort, so far, has been to make the N-S interface as transparent and clean as possible to enhance the proximity effects. Here, we investigate the influence of interface transparency on the superconducting proximity effect of niobium on gold by probing the local density of states (DOS) at the surface of a thin gold layer deposited on a thick niobium film. This is achieved by measuring the tunneling spectra with a home-made STM operating in a upside-down dilution fridge at 60mK temperature footnote[1]N. Moussy, H. Courtois, and B. Pannetier, Rev. Sci. Instrum. 72, 128 (2001).. We observe BCS like spectra for the transparent interface since the gold layer thickness is much smaller than the coherence length. The interface transparency is adjusted by in situ argon etching of the Nb surface before depositing the gold layer. As a result of this semi-transparent interface, the BCS-like spectra evolve into a depression in the DOS near the Fermi energy (E_F) with a reduced energy scale as compared to the superconducting gap (Δ) together with some remnant features near Δ. We discuss the dependence of these two features on the interface transparency and the gold layer thickness and its possible implications.

  19. The thermal conductivity of high modulus Zylon fibers between 400 mK and 4 K

    NASA Astrophysics Data System (ADS)

    Wikus, Patrick; Figueroa-Feliciano, Enectalí; Hertel, Scott A.; Leman, Steven W.; McCarthy, Kevin A.; Rutherford, John M.

    2008-11-01

    Zylon is a synthetic polyurethane polymer fiber featuring very high mechanical strength. Measurements of the thermal conductivity λZ(T) of high modulus Zylon fibers at temperatures between 400 mK and 4 K were performed to assess if they can be successfully employed in the design of high performance suspension systems for cold stages of adiabatic demagnetization refrigerators. The linear mass density of the yarn used in these measurements amounts to 3270 dtex, which is also a measure for the yarn's cross section. The experimental data for the thermal conductivity was fitted to a function of the form λZ=(1010±30)·TpWmmdtexK. This result was normalized to the breaking strength of the fibers and compared with Kevlar. It shows that Kevlar outperforms Zylon in the investigated temperature range. At 1.5 K, the thermal conductivity integral of Zylon yarn is twice as high as the thermal conductivity integral of Kevlar yarn with the same breaking strength. A linear mass density of 1 tex is equivalent to a yarn mass of 1 g/km. High modulus Zylon has a density of 1.56 g/cm 3.

  20. Nature or Nurture: the peculiar HH 900 jet and outflow system in the Carina nebula

    NASA Astrophysics Data System (ADS)

    Reiter, Megan; Smith, Nathan; Kiminki, Megan M.; Bally, John

    2015-01-01

    We present new optical and IR spectroscopy and Hubble Space Telescope imaging of HH 900, a peculiar protostellar outflow in the Carina nebula. Previous Hα imaging from HST revealed an unusually broad, bipolar outflow emerging from a small, tadpole-shaped globule that is illuminated by the many O-type stars in nearby Trumpler 16. Near-IR narrowband [Fe II] images reveal a symmetric, collimated jet that bisects the broad outflow traced by Hα. In a giant H II region like Carina, [Fe II] emission traces dense gas that is self-shielded from Lyman continuum photons from nearby O-type stars, but is excited by non-ionizing FUV photons that penetrate the ionization front within the jet. New Gemini AO images of near-IR H2 emission show that molecules survive in the outflow, and follow the Hα morphology. Position-velocity diagrams of the three lines also reveal very different kinematics. [Fe II] traces steady, jet-like velocities that are faster than those observed in H2 emission. Most strikingly, Hα velocities resemble the Hubble wedges seen in the position-velocity diagrams of some molecular outflows, but few other protostellar jets. We propose that [Fe II] emission traces the protostellar jet itself while H2 emission reveals the molecules that (briefly) survive in the outflow, and Hα traces the ionized skin of the outflow sheath entrained by the jet. The high estimated mass-loss rate of the jet requires a high accretion rate, implying that the unseen driving source is an intermediate-mass (~2-8 Msun) protostar. We propose that HH 900 provides a bridge between molecular outflows driven by deeply embedded sources, and jets from unobscured low-mass protostars because external irradiation from nearby O-type stars illuminates both the collimated atomic jet core and the material it sweeps up.

  1. Measurement of b-jet Shapes in Inclusive Jet Production in p anti-p Collisions at s**(1/2) = 1.96-TeV

    SciTech Connect

    Aaltonen, T.

    2008-06-01

    We present a measurement of the shapes of b-jets using 300 pb{sup -1} of data obtained with the upgraded Collider Detector at Fermilab (CDF II) in p{bar p} collisions at center of mass energy {radical}s = 1.96 TeV. This measurement covers a wide transverse momentum range, from 52 to 300 GeV/c. Samples of heavy-flavor enhanced jets together with inclusive jets are used to extract the average shapes of b-jets. The b-jets are expected to be broader than inclusive jets. Moreover, b-jets containing a single b-quark are expected to be narrower than those containing a b{bar b} pair from gluon splitting. The measured b-jet shapes are found to be significantly broader than expected from the pythia and HERWIG Monte Carlo simulations. This effect may arise from an underestimation of the fraction of b-jets originating from gluon splitting in these simulations.

  2. Plasma confinement at JET

    NASA Astrophysics Data System (ADS)

    Nunes, I.; JET Contributors

    2016-01-01

    Operation with a Be/W wall at JET (JET-ILW) has an impact on scenario development and energy confinement with respect to the carbon wall (JET-C). The main differences observed were (1) strong accumulation of W in the plasma core and (2) the need to mitigate the divertor target temperature to avoid W sputtering by Be and other low Z impurities and (3) a decrease of plasma energy confinement. A major difference is observed on the pedestal pressure, namely a reduction of the pedestal temperature which, due to profile stiffness the plasma core temperature is also reduced leading to a degradation of the global confinement. This effect is more pronounced in low β N scenarios. At high β N, the impact of the wall on the plasma energy confinement is mitigated by the weaker plasma energy degradation with power relative to the IPB98(y, 2) scaling calculated empirically for a CFC first wall. The smaller tolerable impurity concentration for tungsten (<10-5) compared to that of carbon requires the use of electron heating methods to prevent W accumulation in the plasma core region as well as gas puffing to avoid W entering the plasma core by ELM flushing and reduction of the W source by decreasing the target temperature. W source and the target temperature can also be controlled by impurity seeding. Nitrogen and Neon have been used and with both gases the reduction of the W source and the target temperature is observed. Whilst more experiments with Neon are necessary to assess its impact on energy confinement, a partial increase of plasma energy confinement is observed with Nitrogen, through the increase of edge temperature. The challenge for scenario development at JET is to extend the pulse length curtailed by its transient behavior (W accumulation or MHD), but more importantly by the divertor target temperature limits. Re-optimisation of the scenarios to mitigate the effect of the change of wall materials maintaining high global energy confinement similar to JET-C is

  3. JetStar

    NASA Technical Reports Server (NTRS)

    1981-01-01

    The Dryden C-140 JetStar during testing of advanced propfan designs. Dryden conducted flight research in 1981-1982 on several designs. The technology was developed under the direction of the Lewis Research Center (today the Glenn Research Center, Cleveland, OH) under the Advanced Turboprop Program. Under that program, Langley Research Center in Virginia oversaw work on accoustics and noise reduction. These efforts were intended to develop a high-speed and fuel-efficient turboprop system. NASA's Dryden Flight Research Facility (later the Dryden Flight Research Center, Edwards, CA), in co-operation with the Lewis Research Center, investigated the acoustic characteristics of a series of subscale advanced design propellors in the early eighties. These propellors were designed to rotate at a tip speed faster than the speed of sound. They are, in effect, a 'swept back wing' version of a propellor. The tests were conducted on Dryden's C-140 Jetstar, seen here on a research flight over the Mojave desert. The JetStar was modified with the installation of an air turbine drive system. The drive motor, with a 24 inch test propellor, was mounted in a pylon atop the JetStar. The JetStar was equipped with an array of 28 microphones flush-mounted in the fuselage of the aircraft beneath the propellor. Microphones mounted on the wings and on accompanying chase aircraft provided far-field acoustic data. In the 1960s, the same JetStar was equipped with an electronic variable stability flight control system. Called the General Purpose Airborne Simulator (GPAS), the aircraft could duplicate the flight characteristics of a wide variety of advanced aircraft and was used for supersonic transport and general aviation research and as a training and support system for Space Shuttle Approach and Landing Tests at Dryden in 1977. In 1985, the JetStar's wings were modified with suction and spray devices in a laminar (smooth) air flow program to study ways of improving the flow of air over the

  4. Coronal Jets in Closed Magnetic Regions on the Sun

    NASA Astrophysics Data System (ADS)

    Wyper, Peter Fraser; DeVore, C. R.

    2015-04-01

    Coronal jets are dynamic, collimated structures observed in solar EUV and X-ray emission. They appear predominantly in the open field of coronal holes, but are also observed in areas of closed field, especially active regions. A common feature of coronal jets is that they originate from the field above a parasitic polarity of opposite sign to the surrounding field. Some process - such as instability onset or flux emergence - induces explosive reconnection between the closed “anemone” field and the surrounding open field that generates the jet. The lesser number of coronal jets in closed-field regions suggests a possible stabilizing effect of the closed configuration with respect to coronal jet formation. If the scale of the jet region is small compared with the background loop length, as in for example type II spicules, the nearby magnetic field may be treated as locally open. As such, one would expect that if a stabilizing effect exists it becomes most apparent as the scale of the anemone region approaches that of the background coronal loops.To investigate if coronal jets are indeed suppressed along shorter coronal loops, we performed a number of simulations of jets driven by a rotation of the parasitic polarity (as in the previous open-jet calculations by Pariat et. al 2009, 2010, 2015) embedded in a large-scale closed bipolar field. The simulations were performed with the state of the art Adaptively Refined Magnetohydrodynamics Solver. We will report here how the magnetic configuration above the anemone region determines the nature of the jet, when it is triggered, and how much of the stored magnetic energy is released. We show that regions in which the background field and the parasitic polarity region are of comparable scale naturally suppress explosive energy release. We will also show how in the post-jet relaxation phase a combination of confined MHD waves and weak current layers are generated by the jet along the background coronal loops, both of which

  5. A Minor-Merger Interpretation for NGC 1097's ``Jets''

    NASA Astrophysics Data System (ADS)

    Higdon, James L.; Wallin, John F.

    2003-03-01

    while passing through NGC 1097's disk, accounting for the jets' lack of H I and H II. The remnants' (B-V) color would still agree with observations even after ~3 Gyr of passive evolution, provided the cannibalized dwarf was low-metallicity and dominated by young stars at impact.

  6. Jet Signatures in the Spectra of Accreting Black Holes

    NASA Astrophysics Data System (ADS)

    O' Riordan, Michael; Pe'er, Asaf; McKinney, Jonathan C.

    2016-03-01

    Jets are observed as radio emission in active galactic nuclei and during the low/hard state in X-ray binaries (XRBs), but their contribution at higher frequencies has been uncertain. We study the dynamics of jets in XRBs using the general-relativistic magnetohydrodynamic code HARM. We calculate the high-energy spectra and variability properties using a general-relativistic radiative transport code based on grmonty. We find the following signatures of jet emission: (i) a significant γ-ray peak above ˜1022 Hz, (ii) a break in the optical/UV spectrum, with a change from ν {L}ν ˜ {ν }0 to ν {L}ν ˜ ν , followed by another break at higher frequencies where the spectrum roughly returns to ν {L}ν ˜ {ν }0, and (iii) a pronounced synchrotron peak near or below ˜1014 Hz indicates that a significant fraction of any observed X-ray emission originates in the jet. We investigate the variability during a large-scale magnetic field inversion in which the Blandford-Znajek (BZ) jet is quenched and a new transient hot reconnecting plasmoid is launched by the reconnecting field. The ratio of the γ-rays to X-rays changes from {L}γ /{L}{{X}}\\gt 1 in the BZ jet to {L}γ /{L}{{X}}\\lt 1 during the launching of the transient plasmoid.

  7. Minkowski's Object: A Starburst Triggered by a Radio Jet, Revisited

    SciTech Connect

    Croft, S; van Breugel, W; de Vries, W; Dopita, M; Martin, C; Morganti, R; Neff, S; Oosterloo, T; Schiminovich, D; Stanford, S A; van Gorkom, J

    2006-02-07

    We present neutral hydrogen, ultraviolet, optical and near-infrared imaging, as well as optical spectroscopic observations of Minkowski's Object (MO), a star forming system at the end of a radio jet associated with NGC541 at the center of a cluster of galaxies, Abell 194. The observations strengthen the evidence, first presented in 1985, that MO is a small, peculiar galaxy in which the star formation has been triggered by the radio jet. Key new results are the discovery of a double H I cloud with a mass of 4.9 x 10{sup 8} M{sub {circle_dot}} straddling the radio jet downstream from MO at the location where the jet changes direction and decollimates; a strong detection of MO at near- and far-UV wavelengths and in H{alpha}, both of which also show a double structure; and what appear to be numerous H II regions and associated clusters in MO. The UV morphology of MO along the radio jet resembles the radio-aligned, rest-frame UV morphologies seen in many high redshift radio galaxies, which are also thought to be caused by jet-induced star formation. The UV, optical, and near-IR data show that the stellar population is dominated by a 7.5Myr-old instantaneous burst, with a total stellar mass of 1.9 x 10{sup 7} M{sub {circle_dot}}.

  8. Experiments in axisymmetric supersonic jets

    NASA Astrophysics Data System (ADS)

    Moore, Cyrille Dennis

    An experimental study of the effects of exit Mach number and density ratio on the development of axisymmetric jets is described in this thesis. Jet exit Mach numbers of 1.41, 2.0, and 3.0, were studied for jets of helium, argon, and nitrogen. The jets exit into a gas at rest (velocity ratio = 0), in order to better isolate the effects of compressibility and density ratio. Density ratios vary from 0.23 to 5.5.In order to generate shock free-jets, unique nozzles were designed and constructed for each gas and Mach number combination. A plating method for the construction of the nozzles was developed to ensure high-accuracy and a good surface finish at a cost significantly less than direct-machining techniques.The spreading rate of the jet for several downstream locations is measured with a pitot probe. Centerline data are used to characterise the length of the potential core of the jet, which correlates well with the relative spreading rates. Limited frequency data is obtained through the use of piezo-resistive pressure probes. This method is promising for flows that are not conducive to hot-wire probes.Spark shadography is used to visualize both the mean and instantaneous flow, with the minimum spark time being 20 nanoseconds. The convection velocity of large-scale disturbances is estimated from the visible Mach-type acoustic waves emanating from the jet.For a wide range of jet Mach and Reynolds numbers, the convection velocity of the large scale disturbances in the potential core region of the jet is approximately 0.8 times the jet velocity, the approximate velocity of the first helical instability mode of the jet.The main objectives of the present work were to investigate the effects of compressibility and density on the initial development of the axisymmetric jet. Although the data are not sufficient to determine if the convective Mach number concept used in 2-d shear layer research will work in the case of an axisymmetric jet, it is clear that the axisymmetric

  9. SparkJet Efficiency

    NASA Technical Reports Server (NTRS)

    Golbabaei-Asl, Mona; Knight, Doyle; Anderson, Kellie; Wilkinson, Stephen

    2013-01-01

    A novel method for determining the thermal efficiency of the SparkJet is proposed. A SparkJet is attached to the end of a pendulum. The motion of the pendulum subsequent to a single spark discharge is measured using a laser displacement sensor. The measured displacement vs time is compared with the predictions of a theoretical perfect gas model to estimate the fraction of the spark discharge energy which results in heating the gas (i.e., increasing the translational-rotational temperature). The results from multiple runs for different capacitances of c = 3, 5, 10, 20, and 40 micro-F show that the thermal efficiency decreases with higher capacitive discharges.

  10. Plasma jet takes off.

    PubMed Central

    Frazer, L

    1999-01-01

    Thanks to a series of joint research projects by Los Alamos National Laboratory, Beta Squared of Allen, Texas, and the University of California at Los Angeles, there is now a more environmentally sound method for cleaning semiconductor chips that may also be effective in cleaning up chemical, bacterial, and nuclear contaminants. The Atmospheric Pressure Plasma Jet uses a type of ionized gas called plasma to clean up contaminants by binding to them and lifting them away. In contrast to the corrosive acids and chemical solvents traditionally used to clean semiconductor chips, the jet oxidizes contaminants, producing only benign gaseous by-products such as oxygen and carbon dioxide. The new technology is also easy to transport, cleans thoroughly and quickly, and presents no hazards to its operators. PMID:10417375

  11. Jet decorrelation and jet shapes at the Tevatron

    SciTech Connect

    Heuring, T.C.

    1996-07-01

    We present results on measurements of jet shapes and jet azimuthal decorrelation from {bar p}P collisions at {radical}s = 1.8 TeV using data collected during the 1992-1993 run of the Fermilab Tevatron. Jets are seen to narrow both with increasing Awe {sub TTY} and increasing rapidity. While HERWIG, a puritan shower Monte Carlo, predicts slightly narrower jets, it describes the trend of the data well; NO CD described qualitative features of the data but is sensitive to both renormalization scale and jet definitions. Jet azimuthal decorrelation has been measured out to five units of pseudorapidity. While next-to-leading order CD and a leading-log approximation based on BFKL resummation fail to reproduce the effect, HERWIG describes the data well.

  12. Far Noise Field of Air Jets and Jet Engines

    NASA Technical Reports Server (NTRS)

    Callaghan, Edmund E; Coles, Willard D

    1957-01-01

    An experimental investigation was conducted to study and compare the acoustic radiation of air jets and jet engines. A number of different nozzle-exit shapes were studied with air jets to determine the effect of exit shape on noise generation. Circular, square, rectangular, and elliptical convergent nozzles and convergent-divergent and plug nozzles were investigated. The spectral distributions of the sound power for the engine and the air jet were in good agreement for the case where the engine data were not greatly affected by reflection or jet interference effects. Such power spectra for a subsonic or slightly choked engine or air jet show that the peaks of the spectra occur at a Strouhal number of 0.3.

  13. Micromachined chemical jet dispenser

    SciTech Connect

    Swierkowski, S.; Ciarlo, D.

    1996-05-13

    Goal is to develop a multi-channel micromachined chemical fluid jet dispenser that is applicable to prototype tests with biological samples that demonstrate its utility for molecular biology experiments. Objective is to demonstrate a new device capable of ultrasonically ejecting droplets from 10-200 {mu}m diameter capillaries that are arranged in an array that is linear or focused. The device is based on several common fabrication procedures used in MEMS (micro electro mechanical systems) technology: piezoelectric actuators, silicon, etc.

  14. Alternative jet aircraft fuels

    NASA Technical Reports Server (NTRS)

    Grobman, J.

    1979-01-01

    Potential changes in jet aircraft fuel specifications due to shifts in supply and quality of refinery feedstocks are discussed with emphasis on the effects these changes would have on the performance and durability of aircraft engines and fuel systems. Combustion characteristics, fuel thermal stability, and fuel pumpability at low temperature are among the factors considered. Combustor and fuel system technology needs for broad specification fuels are reviewed including prevention of fuel system fouling and fuel system technology for fuels with higher freezing points.

  15. Vortex diode jet

    SciTech Connect

    Houck, E.D.

    1994-05-17

    A fluid transfer system is described that combines a vortex diode with a jet ejector to transfer liquid from one tank to a second tank by a gas pressurization method having no moving mechanical parts in the fluid system. The vortex diode is a device that has a high resistance to flow in one direction and a low resistance to flow in the other. 10 figures.

  16. Dose-dependent pharmacokinetics and efficacy of MK-401 against old, and young-mature infections of Fasciola hepatica in the rat.

    PubMed

    Schulman, M D; Valentino, D; Cifelli, S; Ostlind, D A

    1982-08-01

    The dose-dependent pharmacokinetics and the efficacy of MK-401 (4-amino-6-trichloroethenyl-1,3-benzenedisulfonamide) against old and young-mature infections of Fasciola hepatica were studied in experimentally infected rats. Fractionation of the host's blood after administration of 14C-MK-401 (0.77-15.8 mg/kg) showed that MK-401 was bound predominately to erythrocytes at doses below 4 mg/kg and at higher doses was distributed equally between the red cells and the plasma. Maximum amounts of MK-401 in the blood occurred 2 to 4 hr postadministration and were a hyperbolic function of dose, increasing almost linearly with dose up to 6 mg/kg and then beginning to saturate. Drug uptake by F. hepatica occurred at all doses and increased in direct proportion to the blood level. A single oral dose of MK-401 at 5 mg/kg was found to be highly effective (89%) against older infections (39-44 wk) but was virtually ineffective (1.5%) against younger flukes (9-16 wk). After administration of 14C-MK-401 at 5 mg/kg, drug concentrations in the blood and flukes of rats harboring older infections were significantly higher than those in the blood and flukes of rats with younger infections. Virtually identical differences in the blood level of MK-401 were observed in young and in old, noninfected rats after administration of 14C-MK-401 at 5 mg/kg. The increased efficacy of MK-401 against older infections of F. hepatica in the rat may be related to the age of the host rather than the parasite. PMID:7119988

  17. The efficacy of the Wee1 inhibitor MK-1775 combined with temozolomide is limited by heterogeneous distribution across the blood-brain barrier in glioblastoma

    PubMed Central

    Pokorny, Jenny L.; Calligaris, David; Gupta, Shiv K.; Iyekegbe, Dennis O.; Mueller, Dustin; Bakken, Katrina K.; Carlson, Brett L.; Schroeder, Mark A.; Evans, Debra L.; Lou, Zhenkun; Decker, Paul A.; Eckel-Passow, Jeanette E.; Pucci, Vincenzo; Ma, Bennett; Shumway, Stuart D.; Elmquist, William; Agar, Nathalie Y.; Sarkaria, Jann N.

    2015-01-01

    Purpose Wee1 regulates key DNA damage checkpoints, and in this study, the efficacy of the Wee1 inhibitor MK-1775 was evaluated in GBM xenograft models alone and in combination with radiation and/or temozolomide (TMZ). Experimental design In vitro MK-1775 efficacy alone and in combination with TMZ, and the impact on DNA damage was analyzed by western blotting and γH2AX foci formation. In vivo efficacy was evaluated in orthotopic and heterotopic xenografts. Drug distribution was assessed by conventional mass spectrometry (MS) and matrix-assisted laser desorption/ionization (MALDI) -MS imaging. Results GBM22 (IC50 = 68 nM) was significantly more sensitive to MK-1775 compared to 5 other GBM xenograft lines including GBM6 (IC50 >300 nM), and this was associated with a significant difference in pan-nuclear γH2AX staining between treated GBM22 (81% cells positive) and GBM6 (20% cells positive) cells. However, there was no sensitizing effect of MK-1775 when combined with TMZ in vitro. In an orthotopic GBM22 model, MK-1775 was ineffective when combined with TMZ, while in a flank model of GBM22, MK-1775 exhibited both single agent and combinatorial activity with TMZ. Consistent with limited drug delivery into orthotopic tumors, the normal brain to whole blood ratio following a single MK-1775 dose was 5%, and MALDI-MS imaging demonstrated heterogeneous and markedly lower MK-1775 distribution in orthotopic as compared to heterotopic GBM22 tumors. Conclusions Limited distribution to brain tumors may limit the efficacy of MK-1775 in GBM. PMID:25609063

  18. Arc jet diagnostics tests

    NASA Technical Reports Server (NTRS)

    Willey, Ronald J.

    1989-01-01

    Two objectives were addressed during a 10 week 1988 NASA/ASEE summer faculty fellowship at the Johnson Space Center Atmospheric Reentry Materials Structures Evaluation Facility (ARMSEF). These objectives were the evaluation of mass spectrometry for the measurement of atomic and molecular species in an arc jet environment, and the determination of atomic recombination coefficients for reaction cured glass (RCG) coated high temperature surface insulation (HRSI) materials subjected to simulated reentry conditions. Evaluation of mass spectrometry for the measurement of atomic and molecular species provided some of the first measurements of point compositions in arc jet tunnel environments. A major objective of this project centered around the sampling residence time. A three staged vacuum sampling system pulled the molecules and atoms from the arc jet to a quadrupole ionization mass spectrometer in 400 milliseconds. Conditions investigated included a composition survey across the nozzle exit at 3 cm z-distance from the nozzle exit for 3 different currents. Also, a point composition survey was taken around a shock created by the presence of a blunt body.

  19. The R Aquarii-jet at UV and radio wavelengths

    NASA Technical Reports Server (NTRS)

    Michalitsianos, A. G.; Kafatos, M.; Hollis, J. M.

    1986-01-01

    The jet and central star of the peculiar symbiotic variable R Aquarii was monitored with IUE low resolution SWP 1200 to 2000 A spectra, in order to detect possible temporal variations. The absolute line intensities of N V, C IV and He II, as well as numerous other ionic species in the jet are found to vary on a timescale of 1.5 yr. High spatial resolution radio VLA maps and SiO maser millimeter interferometer observations of the star are quoted.

  20. Longitudinal Variation and Waves in Jupiter's South Equatorial Wind Jet

    NASA Technical Reports Server (NTRS)

    Simon-Miller, A. A.; Rogers, John H.; Gierasch, Peter J.; Choi, David; Allison, Michael; Adamoli, Gianluigi; Mettig, Hans-Joerg

    2012-01-01

    We have conducted a detailed study of the cloud features in the strong southern equatorial wind jet near 7.5 S planetographic latitude. To understand the apparent variations in average zonal wind jet velocity at this latitude [e.g.. 1,2,3], we have searched for variations iIi both feature latitude and velocity with longitude and time. In particular, we focused on the repetitive chevron-shaped dark spots visible on most dates and the more transient large anticyclonic system known as the South Equatorial Disturbance (SED). These small dark spots are interpreted as cloud holes, and are often used as material tracers of the wind field.

  1. Small-scale chromospheric jets above a sunspot light bridge

    NASA Astrophysics Data System (ADS)

    Louis, Rohan E.; Beck, Christian; Ichimoto, Kiyoshi

    2014-07-01

    Context. The chromosphere above sunspot umbrae and penumbrae shows several different types of fast dynamic events such as running penumbral waves, umbral flashes, and penumbral microjets. Aims: The aim of this paper is to identify the physical driver responsible for the dynamic and small-scale chromospheric jets above a sunspot light bridge. Methods: High-resolution broadband filtergrams of active region NOAA 11271 in Ca ii H and G band were obtained with the Solar Optical Telescope on board Hinode. We identified the jets in the Ca ii H images using a semi-automatic routine and determined their length and orientation. We applied local correlation tracking (LCT) to the G-band images to obtain the photospheric horizontal velocity field. The magnetic field topology was derived from a Milne-Eddington inversion of a simultaneous scan with the Spectropolarimeter. Results: The chromospheric jets consist of a bright, triangular-shaped blob that lies on the light bridge, while the apex of this blob extends into a spike-like structure that is bright against the dark umbral background. Most of the jets have apparent lengths of less than 1000 km and about 30% of the jets have lengths between 1000-1600 km. The jets are oriented within ±35° to the normal of the spine of the light bridge. Most of them are clustered near the central part of the light bridge within a 2'' area. The jets are seen to move rapidly along the light bridge and many of them cannot be identified in successive images taken with a 2 min cadence. The jets are primarily located on one side of the light bridge and are directed into the umbral core. The Stokes profiles at or close to the location of the blobs on the LB exhibit both a significant net circular polarization and multiple components, including opposite-polarity lobes. The magnetic field diverges from the light bridge towards the umbral cores that it separates. The LCT reveals that in the photosphere there is a predominantly uni-directional flow with

  2. An innovative blazar classification based on radio jet kinematics

    NASA Astrophysics Data System (ADS)

    Hervet, O.; Boisson, C.; Sol, H.

    2016-07-01

    Context. Blazars are usually classified following their synchrotron peak frequency (νF(ν) scale) as high, intermediate, low frequency peaked BL Lacs (HBLs, IBLs, LBLs), and flat spectrum radio quasars (FSRQs), or, according to their radio morphology at large scale, FR I or FR II. However, the diversity of blazars is such that these classes seem insufficient to chart the specific properties of each source. Aims: We propose to classify a wide sample of blazars following the kinematic features of their radio jets seen in very long baseline interferometry (VLBI). Methods: For this purpose we use public data from the MOJAVE collaboration in which we select a sample of blazars with known redshift and sufficient monitoring to constrain apparent velocities. We selected 161 blazars from a sample of 200 sources. We identify three distinct classes of VLBI jets depending on radio knot kinematics: class I with quasi-stationary knots, class II with knots in relativistic motion from the radio core, and class I/II, intermediate, showing quasi-stationary knots at the jet base and relativistic motions downstream. Results: A notable result is the good overlap of this kinematic classification with the usual spectral classification; class I corresponds to HBLs, class II to FSRQs, and class I/II to IBLs/LBLs. We deepen this study by characterizing the physical parameters of jets from VLBI radio data. Hence we focus on the singular case of the class I/II by the study of the blazar BL Lac itself. Finally we show how the interpretation that radio knots are recollimation shocks is fully appropriate to describe the characteristics of these three classes.

  3. Microwave-Assisted Synthesis of a MK2 Inhibitor by Suzuki-Miyaura Coupling for Study in Werner Syndrome Cells

    PubMed Central

    Bagley, Mark C.; Baashen, Mohammed; Chuckowree, Irina; Dwyer, Jessica E.; Kipling, David; Davis, Terence

    2015-01-01

    Microwave-assisted Suzuki-Miyaura cross-coupling reactions have been employed towards the synthesis of three different MAPKAPK2 (MK2) inhibitors to study accelerated aging in Werner syndrome (WS) cells, including the cross-coupling of a 2-chloroquinoline with a 3-pyridinylboronic acid, the coupling of an aryl bromide with an indolylboronic acid and the reaction of a 3-amino-4-bromopyrazole with 4-carbamoylphenylboronic acid. In all of these processes, the Suzuki-Miyaura reaction was fast and relatively efficient using a palladium catalyst under microwave irradiation. The process was incorporated into a rapid 3-step microwave-assisted method for the synthesis of a MK2 inhibitor involving 3-aminopyrazole formation, pyrazole C-4 bromination using N-bromosuccinimide (NBS), and Suzuki-Miyaura cross-coupling of the pyrazolyl bromide with 4-carbamoylphenylboronic acid to give the target 4-arylpyrazole in 35% overall yield, suitable for study in WS cells. PMID:26046488

  4. MK3TOOLS & NetCDF - storing VLBI data in a machine independent array oriented data format

    NASA Astrophysics Data System (ADS)

    Hobiger, T.; Koyama, Y.; Kondo, T.

    2007-07-01

    In the beginning of 2002 the International VLBI Service (IVS) has agreed to introduce a Platform-independent VLBI exchange format (PIVEX) which permits the exchange of observational data and stimulates the research across different analysis groups. Unfortunately PIVEX has never been implemented and many analysis software packages are still depending on prior processing (e.g. ambiguity resolution and computation of ionosphere corrections) done by CALC/SOLVE. Thus MK3TOOLS which handles MK3 databases without CALC/SOLVE being installed has been developed. It uses the NetCDF format to store the data and since interfaces exist for a variety of programming languages (FORTRAN, C/C++, JAVA, Perl, Python) it can be easily incorporated in existing and upcoming analysis software packages.

  5. Altitude-Wind-Tunnel Investigation of the 19B-2, 19B-8, and 19XB-1 Jet-Propulsion Engines. II - Analysis of Turbine Performance of the 19B-8 Engine

    NASA Technical Reports Server (NTRS)

    Krebs, Richard P.; Suozzi, Frank L.

    1947-01-01

    Performance characteristics of the turbine in the 19B-8 jet propulsion engine were determined from an investigation of the complete engine in the Cleveland altitude wind tunnel. The investigation covered a range of simulated altitudes from 5000 to 30,000 feet and flight Mach numbers from 0.05 to 0.46 for various tail-cone positions over the entire operable range of engine speeds. The characteristics of the turbine are presented as functions of the total-pressure ratio across the turbine and the turbine speed and the gas flow corrected to NACA standard atmospheric conditions at sea level. The effect of changes in altitude, flight Mach number, and tail-cone position on turbine performance is discussed. The turbine efficiency with the tail cone in varied from a maximum of 80.5 percent to minimum of 75 percent over a range of engine speeds from 7500 to 17,500 rpm at a flight Mach number of 0.055. Turbine efficiency was unaffected by changes in altitude up to 15,000 feet but was a function of tail-cone position and flight Mach number. Decreasing the tail-pipe-nozzle outlet area 21 percent reduced the turbine efficiency between 2 and 4.5 percent. The turbine efficiency increased between 1.5 and 3 percent as the flight Mach number changed from 0.055 to 0.297.

  6. Vacuum Measurements of a Novel Micro-resonator Based on Tin Whiskers Performed at mK Temperatures

    NASA Astrophysics Data System (ADS)

    Človečko, M.; Gažo, E.; Longauer, S.; Múdra, E.; Skyba, P.; Vavrek, F.; Vojtko, M.

    2014-04-01

    We present a method of preparation and preliminary vacuum measurements conducted at ˜20 mK of a new type of micro-resonator based on Sn-whiskers. Sn-whiskers have ˜1 μm radius and their length can be ˜1-2 mm. As added benefit, the Sn-whiskers are mono-crystalline metal fibers with relatively smooth surface and being superconducting at low temperatures one may expect their high Q-factors.

  7. The p38 MAPK-MK2 axis regulates E2F1 and FOXM1 expression after epirubicin treatment

    PubMed Central

    de Olano, Natalia; Koo, Chuay-Yeng; Monteiro, Lara J.; Pinto, Paola H.; Gomes, Ana R.; Aligue, Rosa; Lam, Eric W.-F.

    2012-01-01

    E2F1 is responsible for the regulation of FOXM1 expression, which plays a key role in epirubicin resistance. In here, we examined the role and regulation of E2F1 in response to epirubicin in cancer cells. We first demonstrated that E2F1 plays a key role in promoting FOXM1 expression, cell survival and epirubicin resistance as its depletion by siRNA attenuated FOXM1 induction and cell viability in response to epirubicin. We also found that the p38-MAPK activity mirrors the expression patterns of E2F1 and FOXM1 in both epirubicin sensitive and resistant MCF-7 breast cancer cells, suggesting p38 has a role in regulating E2F1 expression and epirubicin resistance. Consistently, studies using pharmacological inhibitors, siRNA knockdown and knockout MEFs revealed that p38 mediates the E2F1 induction by epirubicin and that the induction of E2F1 by p38 is in turn mediated through its downstream kinase MK2 (MAPK-activated protein kinase 2; MAPKAPK2). In agreement, in vitro phosphorylation assays showed that MK2 can directly phosphorylate E2F1 at Ser-364. Transfection assays also demonstrated that E2F1 phosphorylation at Ser-364 participates in its induction by epirubicin, but also suggests that other phosphorylation events are also involved. In addition, the p38-MK2 axis can also limit JNK induction by epirubicin and notably, JNK represses FOXM1 expression. Collectively, these findings underscore the importance of p38-MK2 signalling in the control of E2F1 and FOXM1 expression as well as epirubicin sensitivity. PMID:22802261

  8. The induction of polyploidy or apoptosis by the Aurora A kinase inhibitor MK8745 is p53-dependent

    PubMed Central

    Ho, Alan L; Schwartz, Gary K

    2012-01-01

    Aurora kinases are mitotic serine/threonine protein kinases and are attractive novel targets for anticancer therapy. Many small-molecule inhibitors of Aurora kinases are currently undergoing clinical trials. Aurora A kinase is essential for successful mitotic transition. MK8745 is a novel and selective small-molecule inhibitor of Aurora A kinase. MK8745 induced apoptotic cell death in a p53-dependent manner when tested in vitro in cell lines of multiple lineages. Cells expressing wild-type p53 showed a short delay in mitosis followed by cytokinesis, resulting in 2N cells along with apoptosis. However, cells lacking or with mutant p53 resulted in a prolonged arrest in mitosis followed by endoreduplication and polyploidy. Cytokinesis was completely inhibited in p53-deficient cells, as observed by the absence of 2N cell population. The induction of apoptosis in p53-proficient cells was associated with activation of caspase 3 and release of cytochrome c but was independent of p21. Exposure of p53 wild-type cells to MK8745 resulted in the induction of p53 phosphorylation (ser15) and an increase in p53 protein expression. p53-dependent apoptosis by MK8745 was further confirmed in HCT 116 p53−/− cells transfected with wild-type p53. Transient knockdown of Aurora A by specific siRNA recapitulated these p53-dependent effects, with greater percent induction of apoptosis in p53 wild-type cells. In conclusion, our studies show p53 as a determining factor for induction of apoptosis vs. polyploidy upon inhibition of Aurora A. PMID:22293494

  9. MK-801-induced deficits in social recognition in rats: reversal by aripiprazole, but not olanzapine, risperidone, or cannabidiol.

    PubMed

    Deiana, Serena; Watanabe, Akihito; Yamasaki, Yuki; Amada, Naoki; Kikuchi, Tetsuro; Stott, Colin; Riedel, Gernot

    2015-12-01

    Deficiencies in social activities are hallmarks of numerous brain disorders. With respect to schizophrenia, social withdrawal belongs to the category of negative symptoms and is associated with deficits in the cognitive domain. Here, we used the N-methyl-D-aspartate receptor antagonist dizocilpine (MK-801) for induction of social withdrawal in rats and assessed the efficacy of several atypical antipsychotics with different pharmacological profiles as putative treatment. In addition, we reasoned that the marijuana constituent cannabidiol (CBD) may provide benefit or could be proposed as an adjunct treatment in combination with antipsychotics. Hooded Lister rats were tested in the three-chamber version for social interaction, with an initial novelty phase, followed after 3 min by a short-term recognition memory phase. No drug treatment affected sociability. However, distinct effects on social recognition were revealed. MK-801 reduced social recognition memory at all doses (>0.03 mg/kg). Predosing with aripiprazole dose-dependently (2 or 10 mg/kg) prevented the memory decline, but doses of 0.1 mg/kg risperidone or 1 mg/kg olanzapine did not. Intriguingly, CBD impaired social recognition memory (12 and 30 mg/kg) but did not rescue the MK-801-induced deficits. When CBD was combined with protective doses of aripiprazole (CBD-aripiprazole at 12 :  or 5 : 2 mg/kg) the benefit of the antipsychotic was lost. At the same time, activity-related changes in behaviour were excluded as underlying reasons for these pharmacological effects. Collectively, the combined activity of aripiprazole on dopamine D2 and serotonin 5HT1A receptors appears to provide a significant advantage over risperidone and olanzapine with respect to the rescue of cognitive deficits reminiscent of schizophrenia. The differential pharmacological properties of CBD, which are seemingly beneficial in human patients, did not back-translate and rescue the MK-801-induced social memory deficit. PMID:26287433

  10. Synthetic Jets in Cross-flow. Part 1; Round Jet

    NASA Technical Reports Server (NTRS)

    Zaman, K. B. M. Q.; Milanovic, Ivana M.

    2003-01-01

    Results of an experimental investigation on synthetic jets from round orifices with and without cross-flow are presented. Jet Reynolds number up to 46,000 with a fully turbulent approach boundary layer, and Stokes number up to 400. are covered. The threshold of stroke length for synthetic jet formation. in the absence of the cross-flow, is found to be Lo /D approximately 0.5. Above Lo /D is approximately 10, the profiles of normalized centerline mean velocity appear to become invariant. It is reasoned that the latter threshold may be related to the phenomenon of saturation of impulsively generated vortices. In the presence of the cross-flow, the penetration height of a synthetic jet is found to depend on the momentum- flux ratio . When this ratio is defined in terms of the maximum jet velocity and the cross-flow velocity. not only all data collapse but also the jet trajectory is predicted well by correlation equation available for steady jets-in-cross-flow. Distributions of mean velocity, streamwise vorticity as well as turbulence intensity for a synthetic jet in cross-flow are found to be similar to those of a steady jet-in-cross-flow. A pair of counter-rotating streamwise vortices, corresponding to the bound vortex pair of the steady case, is clearly observed. Mean velocity distribution exhibits a dome of low momentum fluid pulled up from the boundary layer, and the entire domain is characterized by high turbulence.

  11. Effect of clozapine on locomotor activity and anxiety-related behavior in the neonatal mice administered MK-801

    PubMed Central

    Pinar, Neslihan; Akillioglu, Kubra; Sefil, Fatih; Alp, Harun; Sagir, Mustafa; Acet, Ahmet

    2015-01-01

    Atypical antipsychotics have been used to treat fear and anxiety disturbance that are highly common in schizophrenic patients. It is suggested that disruptions of N-methyl-d-aspartate (NMDA)-mediated transmission of glutamate may underlie the pathophysiology of schizophrenia. The present study was conducted to analyze the effectiveness of clozapine on the anxiety-related behavior and locomotor function of the adult brain, which had previously undergone NMDA receptor blockade during a developmental period. In order to block the NMDA receptor, male mice were administered 0.25 mg/kg of MK-801 on days 7 to 10 postnatal. In adulthood, they were administered intraperitoneally 0.5 mg/kg of clozapine and tested with open-field and elevated plus maze test, to assess their emotional behavior and locomotor activity. In the group receiving MK-801 in the early developmental period the elevated plus maze test revealed a reduction in the anxiety-related behavior (p<0.05), while the open-field test indicated a decrease in locomotor activity (p<0.01). Despite these reductions, clozapine could not reverse the NMDA receptor blockade. Also, as an atypical antipsychotic agent, clozapine could not reverse impairment in the locomotor activity and anxiety-related behavior, induced by administration of the MK-801 in neonatal period. PMID:26295298

  12. A 10 mK scanning tunneling microscope operating in ultra high vacuum and high magnetic fields.

    PubMed

    Assig, Maximilian; Etzkorn, Markus; Enders, Axel; Stiepany, Wolfgang; Ast, Christian R; Kern, Klaus

    2013-03-01

    We present design and performance of a scanning tunneling microscope (STM) that operates at temperatures down to 10 mK providing ultimate energy resolution on the atomic scale. The STM is attached to a dilution refrigerator with direct access to an ultra high vacuum chamber allowing in situ sample preparation. High magnetic fields of up to 14 T perpendicular and up to 0.5 T parallel to the sample surface can be applied. Temperature sensors mounted directly at the tip and sample position verified the base temperature within a small error margin. Using a superconducting Al tip and a metallic Cu(111) sample, we determined an effective temperature of 38 ± 1 mK from the thermal broadening observed in the tunneling spectra. This results in an upper limit for the energy resolution of ΔE = 3.5 kBT = 11.4 ± 0.3 μeV. The stability between tip and sample is 4 pm at a temperature of 15 mK as demonstrated by topography measurements on a Cu(111) surface. PMID:23556826

  13. Isolation and Characterization of a Novel Imidacloprid-Degrading Mycobacterium sp. Strain MK6 from an Egyptian Soil.

    PubMed

    Kandil, Mahrous M; Trigo, Carmen; Koskinen, William C; Sadowsky, Michael J

    2015-05-20

    Thus far, only a small number and types of bacteria with limited ability in degrading imidacloprid have been reported. Also, genes regulating imidacloprid (IMDA) degradation have yet to be discovered. To study this in more detail, an enrichment technique was used to isolate consortia and pure cultures of IMDA-degrading bacteria. Through this approach, we successfully isolated a novel bacterium capable of completely degrading IMDA as a sole nitrogen source. The bacterium was subsequently identified as Mycobacterium sp. strain MK6 by sequence analysis of its 16S rRNA gene (Genbank accession number KR052814 ). BLASTn searches indicated that 16S rRNA gene from Mycobacterium sp. strain MK6 was 99% identical to several Mycobacterium spp. Mycobacterium sp. strain MK6 transformed 99.7% added IMDA (150 μg mL(-1)) in <2 weeks (t1/2 = 1.6 days) to 6-chloronicotinic acid (6-CNA) as its major metabolite. Although the isolated strain and mixed bacterial consortia were able to degrade IMDA, they failed to grow further on 6-CNA, indicating a lack of IMDA mineralization to carbon dioxide. Small amounts of the desnitro-olefin and desnitro-degradates of IMDA were observed during the incubation but did not accumulate in culture medium. PMID:25932751

  14. The effects of the stellar wind and orbital motion on the jets of high-mass microquasars

    NASA Astrophysics Data System (ADS)

    Bosch-Ramon, V.; Barkov, M. V.

    2016-05-01

    Context. High-mass microquasar jets propagate under the effect of the wind from the companion star, and the orbital motion of the binary system. The stellar wind and the orbit may be dominant factors determining the jet properties beyond the binary scales. Aims: This is an analytical study, performed to characterise the effects of the stellar wind and the orbital motion on the jet properties. Methods: Accounting for the wind thrust transferred to the jet, we derive analytical estimates to characterise the jet evolution under the impact of the stellar wind. We include the Coriolis force effect, induced by orbital motion and enhanced by the wind's presence. Large-scale evolution of the jet is sketched, accounting for wind-to-jet thrust transfer, total energy conservation, and wind-jet flow mixing. Results: If the angle of the wind-induced jet bending is larger than its half-opening angle, the following is expected: (i) a strong recollimation shock; (ii) bending against orbital motion, caused by Coriolis forces and enhanced by the wind presence; and (iii) non-ballistic helical propagation further away. Even if disrupted, the jet can re-accelerate due to ambient pressure gradients, but wind entrainment can weaken this acceleration. On large scales, the opening angle of the helical structure is determined by the wind-jet thrust relation, and the wind-loaded jet flow can be rather slow. Conclusions: The impact of stellar winds on high-mass microquasar jets can yield non-ballistic helical jet trajectories, jet partial disruption and wind mixing, shocks, and possibly non-thermal emission. Among other observational diagnostics, such as radiation variability at any band, the radio morphology on milliarcsecond scales can be informative on the wind-jet interaction.

  15. Isolation, characterization and biological evaluation of bioactive metabolites from Nocardia levis MK-VL_113.

    PubMed

    Kavitha, Alapati; Prabhakar, Peddikotla; Narasimhulu, Manchala; Vijayalakshmi, Muvva; Venkateswarlu, Yenamandra; Rao, Karanam Venkateswara; Raju, Venkata Balaraju Subba

    2010-03-31

    An Actinomycete isolate found to be prominent in the laterite soils of Acharya Nagarjuna University (ANU) Campus, Guntur was identified as Nocardia levis MK-VL_113 by 16S rRNA analysis. Cultural, morphological and physiological characteristics of the strain were recorded. Screening of secondary metabolites obtained from 4-day old culture broth of the strain led to the isolation of two fractions active against a wide variety of Gram-positive, Gram-negative bacteria and fungi. The structure of the first active fraction was elucidated using FT-IR, EI-MS, (1)H NMR and (13)C NMR spectra and identified as 1-phenylbut-3-ene-2-ol which is first time reported as a natural product. The compound exhibited good antimicrobial potential against the opportunistic and pathogenic bacteria and fungi. The antifungal activity of the strain and its metabolite were further confirmed with in vitro and in vivo studies. Evidence for the antagonism of the strain against Fusarium oxysporum, causing wilt disease in sorghum was demonstrated by the formation of inhibition zone in in vitro plate assay and reduction in the incidence of wilt of sorghum plants by using a green house trial. Analysis of the rhizosphere soil extracts by high performance liquid chromatography also demonstrated the production of the compound by the strain under in vivo conditions. As compared to the commercial fungicide mancozeb, the bioactive compound, 1-phenylbut-3-ene-2-ol was highly effective in controlling wilt of sorghum. Besides, the partially purified second fraction (PPF) subjected to gas chromatography-mass spectrometry revealed the presence of phenylethyl alcohol, dibutyl phthalate and 1,2-benzenedicarboxylic acid, 3-nitro. PMID:19577444

  16. 30 CFR 56.7801 - Jet drills.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Jet drills. 56.7801 Section 56.7801 Mineral... HEALTH SAFETY AND HEALTH STANDARDS-SURFACE METAL AND NONMETAL MINES Drilling and Rotary Jet Piercing Rotary Jet Piercing § 56.7801 Jet drills. Jet piercing drills shall be provided with— (a) A system...

  17. 30 CFR 57.7801 - Jet drills.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Jet drills. 57.7801 Section 57.7801 Mineral... HEALTH SAFETY AND HEALTH STANDARDS-UNDERGROUND METAL AND NONMETAL MINES Drilling and Rotary Jet Piercing Rotary Jet Piercing-Surface Only § 57.7801 Jet drills. Jet piercing drills shall be provided with: (a)...

  18. 30 CFR 57.7801 - Jet drills.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Jet drills. 57.7801 Section 57.7801 Mineral... HEALTH SAFETY AND HEALTH STANDARDS-UNDERGROUND METAL AND NONMETAL MINES Drilling and Rotary Jet Piercing Rotary Jet Piercing-Surface Only § 57.7801 Jet drills. Jet piercing drills shall be provided with: (a)...

  19. 30 CFR 57.7801 - Jet drills.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Jet drills. 57.7801 Section 57.7801 Mineral... HEALTH SAFETY AND HEALTH STANDARDS-UNDERGROUND METAL AND NONMETAL MINES Drilling and Rotary Jet Piercing Rotary Jet Piercing-Surface Only § 57.7801 Jet drills. Jet piercing drills shall be provided with: (a)...

  20. 30 CFR 56.7801 - Jet drills.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Jet drills. 56.7801 Section 56.7801 Mineral... HEALTH SAFETY AND HEALTH STANDARDS-SURFACE METAL AND NONMETAL MINES Drilling and Rotary Jet Piercing Rotary Jet Piercing § 56.7801 Jet drills. Jet piercing drills shall be provided with— (a) A system...

  1. 30 CFR 56.7801 - Jet drills.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Jet drills. 56.7801 Section 56.7801 Mineral... HEALTH SAFETY AND HEALTH STANDARDS-SURFACE METAL AND NONMETAL MINES Drilling and Rotary Jet Piercing Rotary Jet Piercing § 56.7801 Jet drills. Jet piercing drills shall be provided with— (a) A system...

  2. 30 CFR 56.7801 - Jet drills.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Jet drills. 56.7801 Section 56.7801 Mineral... HEALTH SAFETY AND HEALTH STANDARDS-SURFACE METAL AND NONMETAL MINES Drilling and Rotary Jet Piercing Rotary Jet Piercing § 56.7801 Jet drills. Jet piercing drills shall be provided with— (a) A system...

  3. 30 CFR 57.7801 - Jet drills.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Jet drills. 57.7801 Section 57.7801 Mineral... HEALTH SAFETY AND HEALTH STANDARDS-UNDERGROUND METAL AND NONMETAL MINES Drilling and Rotary Jet Piercing Rotary Jet Piercing-Surface Only § 57.7801 Jet drills. Jet piercing drills shall be provided with: (a)...

  4. 30 CFR 57.7801 - Jet drills.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Jet drills. 57.7801 Section 57.7801 Mineral... HEALTH SAFETY AND HEALTH STANDARDS-UNDERGROUND METAL AND NONMETAL MINES Drilling and Rotary Jet Piercing Rotary Jet Piercing-Surface Only § 57.7801 Jet drills. Jet piercing drills shall be provided with: (a)...

  5. 30 CFR 56.7801 - Jet drills.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Jet drills. 56.7801 Section 56.7801 Mineral... HEALTH SAFETY AND HEALTH STANDARDS-SURFACE METAL AND NONMETAL MINES Drilling and Rotary Jet Piercing Rotary Jet Piercing § 56.7801 Jet drills. Jet piercing drills shall be provided with— (a) A system...

  6. Oscillations in solar jets observed with the SOT of Hinode: viscous effects during reconnection

    NASA Astrophysics Data System (ADS)

    Tavabi, E.; Koutchmy, S.

    2014-07-01

    Transverse oscillatory motions and recurrence behavior in the chromospheric jets observed by Hinode/SOT are studied. A comparison is considered with the behavior that was noticed in coronal X-ray jets observed by Hinode/XRT. A jet like bundle observed at the limb in Ca II H line appears to show a magnetic topology that is similar to X-ray jets (i.e., the Eiffel tower shape). The appearance of such magnetic topology is usually assumed to be caused by magnetic reconnection near a null point. Transverse motions of the jet axis are recorded but no clear evidence of twist is appearing from the highly processed movie. The aim is to investigate the dynamical behavior of an incompressible magnetic X-point occurring during the magnetic reconnection in the jet formation region. The viscous effect is specially considered in the closed line-tied magnetic X-shape nulls. We perform the MHD numerical simulation in 2-D by solving the visco-resistive MHD equations with the tracing of velocity and magnetic field. A qualitative agreement with Hinode observations is found for the oscillatory and non-oscillatory behaviors of the observed solar jets in both the chromosphere and the corona. Our results suggest that the viscous effect contributes to the excitation of the magnetic reconnection by generating oscillations that we observed at least inside this Ca II H line cool solar jet bundle.

  7. Portrait characteristics of QCD jets

    SciTech Connect

    Dokshitser, Y.L.; Troyan, S.I.; Khoze, V.A.

    1988-01-01

    In the framework of the perturbation-theory approach to the description of multihadron production in hard processes we analyze the portrait characteristics of jets (the energy and the multiplicity distribution in an isolated jet) and their influence on each other (drag effects, the azimuthal asymmetry of the jet in an aggregate). In contrast to the common but theoretically unjustified procedure of analyzing multijet events, we develop a consistent approach based on inclusive and calorimetric characteristics.

  8. Impeller for Water Jet Propulsion

    NASA Technical Reports Server (NTRS)

    2004-01-01

    Marshall Space Flight Center engineers helped North American Marine Jet (NAMJ), Inc. improve the proposed design of a new impeller for jet propulsion system. With a three-dimensional computer model of the new marine jet engine blades, engineers were able to quickly create a solid ploycarbonate model of it. The rapid prototyping allowed the company to avoid many time-consuming and costly steps in creating the impeller.

  9. Jet Physics at the Tevatron

    SciTech Connect

    Bhatti, Anwar; Lincoln, Don

    2010-02-01

    Jets have been used to verify the theory of quantum chromodynamics (QCD), measure the structure of the proton and to search for the physics beyond the Standard Model. In this article, we review the current status of jet physics at the Tevatron, a {radical}s = 1.96 TeV p{bar p} collider at the Fermi National Accelerator Laboratory. We report on recent measurements of the inclusive jet production cross section and the results of searches for physics beyond the Standard Model using jets. Dijet production measurements are also reported.

  10. Jet penetration of high explosive

    SciTech Connect

    Poulsen, P

    1999-08-11

    It is found that a transition between two flow patterns takes place in thick HE targets. In this case, the jet will initially propagate into the HE at the same rate as into an inert material of the same density. The part of the jet that has stagnated and is flowing nearly co-axially with the incoming jet (but at a much lower speed) is being forced toward the surface of the incoming jet by the pressure of the reaction products but has not as yet made contact. After it makes contact, both axial and perpendicular momentum transfer takes place between the two jet components. After this transition, a new steady state will develop for the propagating jet, with the unperturbed front of the jet propagating at a slower rate than previously. The perturbed front of the jet is still propagating at or near the original rate, having had relatively little axial momentum exchange. However, it has acquired radial momentum and is spreading out as it is propagating; it is therefore becoming less capable of penetrating downstream targets. It is the unperturbed part of the jet that is capable of penetrating downstream targets. A calculational method for predicting this case is presented in this report.

  11. Electromagnetic Models of Extragalactic Jets

    SciTech Connect

    Lisanti, M.; Blandford, R.; /KIPAC, Menlo Park

    2007-10-22

    Relativistic jets may be confined by large-scale, anisotropic electromagnetic stresses that balance isotropic particle pressure and disordered magnetic field. A class of axisymmetric equilibrium jet models will be described and their radiative properties outlined under simple assumptions. The partition of the jet power between electromagnetic and mechanical forms and the comoving energy density between particles and magnetic field will be discussed. Current carrying jets may be recognized by their polarization patterns. Progress and prospects for measuring this using VLBI and GLAST observations will be summarized.

  12. Dissipationless decay of Jovian jets

    NASA Astrophysics Data System (ADS)

    Pirraglia, J. A.

    1989-05-01

    IRIS data have been taken as the bases of windshear calculations whose results imply a decrease of the Jovian planet's zonal jets with altitude. The simplified dynamical model developed to furnish a mechanism accounting for the decay involves a highly truncated set of dissipationless equations simulating the upper-tropospheric and stratospheric flow. While the model's lower boundary is constrained as a latitudinally periodic set of alternating jets, the upper boundary constraint maintains a constant potential temperature. The small perturbations to which the imposed zonal jets are unstable grow and interact nonlinearly, generating a zonal flow that opposes the imposed one and thereby leading to the apparent decrease of the jets with altitude.

  13. Field of Flow About a Jet and Effect of Jets on Stability of Jet-Propelled Airplanes

    NASA Technical Reports Server (NTRS)

    Ribner, Herbert S.

    1946-01-01

    A theoretical investigation was conducted on jet-induced flow deviation. Analysis is given of flow inclination induced outside cold and hot jets and jet deflection caused by angle of attack. Applications to computation of effects of jet on longitudinal stability and trim are explained. Effect of jet temperature on flow inclination was found small when thrust coefficient is used as criterion for similitude. The average jet-induced downwash over tail plane was obtained geometrically.

  14. Inhibition by dizocilpine (MK-801) of striatal dopamine release induced by MPTP and MPP+: possible action at the dopamine transporter.

    PubMed

    Clarke, P B; Reuben, M

    1995-01-01

    1. The NMDA-type glutamate receptor antagonist, dizocilpine (MK-801) can protect against neurotoxicity associated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and its principal metabolite, the 1-methyl-4-phenylpyridinium ion (MPP+). It has been suggested that these neurotoxic effects may be mediated by release of excitatory amino acids, but possible alternative mechanisms have been little investigated. 2. MPTP and MPP+ (0.1-1000 microM) were tested in superfused rat striatal synaptosomes preloaded with [3H]-dopamine. Both MPTP (10 microM and higher) and MPP+ (1 microM and higher) evoked an immediate and concentration-dependent release of [3H]-dopamine. The maximal effect exceeded that achievable with nicotine. For subsequent experiments, submaximal concentrations of MPTP (50 microM) and MPP+ (10 microM) were tested. 3. MK-801 (0.1-100 microM) inhibited responses to MPTP (50 microM) and MPP+ (10 microM) in a concentration-dependent manner. However, further tests of NMDA-type glutamate receptor involvement proved negative. Responses to MPTP or MPP+ were unaffected by the omission of Mg2+ or Ca2+ and were not reduced by the NMDA receptor antagonists, AP-7 (200 microM) and kynurenic acid (300 microM). In this assay, N-methyl-D-aspartate (even in the absence of Mg2+ and with added glycine and strychnine) did not evoked [3H]-dopamine release. 4. In crude membrane preparations of rat cerebral cortex, MPTP and MPP+ inhibited high-affinity [3H]-nicotine binding to nicotinic cholinoceptors (IC50 1.8 microM and 26 microM, respectively). 5. [3H]-dopamine release evoked by nicotine (1 microM) was blocked by the nicotinic antagonists,mecamylamine and chlorisondamine, and by MK-801 (all at 100 micro M); K+-evoked release was not affected. Release evoked by MPTP and MPP+ was significantly attenuated by MK-801 but not by mecamylamine or chlorisondamine.6. At a high concentration (1O I1M), the selective dopamine uptake inhibitor, nomifensine, completely blocked [3HJ

  15. Ooishi's Observation: Viewed in the Context of Jet Stream Discovery.

    NASA Astrophysics Data System (ADS)

    Lewis, John M.

    2003-03-01

    Although aircraft encounters with strong westerly winds during World War II provided the stimulus for postwar research on the jet stream, Wasaburo Ooishi observed these winds in the 1920s. Ooishi's work is reviewed in the context of earlier work in upperair observation and postwar work on the jet stream. An effort is made to reconstruct Ooishi's path to the directorship of Japan's first upper-air observatory by reliance on historical studies and memoirs from the Central Meteorological Observatory.Archival records from Japan's Aerological Observatory have been used to document Ooishi's upperair observations. The first official report from the observatory (written in 1926 and in the auxiliary language of Esperanto) assumes a central role in the study. In this report, data are stratified by season and used to produce the mean seasonal wind profiles. The profile for winter gives the first known evidence of the persistent strong westerlies over Japan that would later become known as the jet stream.

  16. A variable jet model for the Hα emission of HH 444

    NASA Astrophysics Data System (ADS)

    Raga, A. C.; Riera, A.; González-Gómez, D. I.

    2010-07-01

    Context. HH 444 is one of the first Herbig-Haro (HH) jets discovered within a photoionized region. Aims: We re-analyze the Hα and red [S II] HST images of HH 444, and calculate the width of the jet as a function of distance from the source. We compare the Hα image with predictions from variable ejection velocity jet models. Methods: The determination of the jet's width is done with a non-parametric, wavelet analysis technique. The axisymmetric, photoionized jet simulations are used to predict Hα maps that can be directly compared with the observations. Results: Starting with a thin jet (unresolved at the resolution of the observations), we are able to produce knots with widths and morphologies that generally agree with the Hα knots of HH 444. This agreement is only obtained if the jet axis is at a relatively large, ~45° angle with respect to the sky. This agrees with previous spectroscopic observations of the HH 444 bow shock, which imply a relatively large jet axis/plane of the sky angle. Conclusions: We conclude that the general morphology of the chain of knots close to V510 Ori (the HH 444 source) can be explained with a variable ejection velocity jet model. For explaining the present positions of the HH 444 knots, however, it is necessary to invoke a more complex ejection velocity history than a single-mode, periodic variability.

  17. Systematic Motion of Fine-scale Jets and Successive Reconnection in Solar Chromospheric Anemone Jet Observed with the Solar Optical Telescope/Hinode

    NASA Astrophysics Data System (ADS)

    Singh, K. A. P.; Isobe, H.; Nishida, K.; Shibata, K.

    2012-11-01

    The Solar Optical Telescope (SOT) on board Hinode allows observations with high spatiotemporal resolution and stable image quality. A λ-shaped chromospheric anemone jet was observed in high resolution with SOT/Hinode. We found that several fine-scale jets were launched from one end of the footpoint to the other. These fine-scale jets (~1.5-2.5 Mm) gradually move from one end of the footpoint to the other and finally merge into a single jet. This process occurs recurrently, and as time progresses the jet activity becomes more and more violent. The time evolution of the region below the jet in Ca II H filtergram images taken with SOT shows that various parts (or knots) appear at different positions. These bright knots gradually merge into each other during the maximum phase. The systematic motion of the fine-scale jets is observed when different knots merge into each other. Such morphology would arise due to the emergence of a three-dimensional twisted flux rope in which the axial component (or the guide field) appears in the later stages of the flux rope emergence. The partial appearance of the knots could be due to the azimuthal magnetic field that appears during the early stage of the flux rope emergence. If the guide field is strong and reconnection occurs between the emerging flux rope and an ambient magnetic field, this could explain the typical feature of systematic motion in chromospheric anemone jets.

  18. Lear jet telescope system

    NASA Technical Reports Server (NTRS)

    Erickson, E. F.; Goorvitch, D.; Dix, M. G.; Hitchman, M. J.

    1974-01-01

    The telescope system was designed as a multi-user facility for observations of celestial objects at infrared wavelengths, where ground-based observations are difficult or impossible due to the effects of telluric atmospheric absorption. The telescope is mounted in a Lear jet model 24B which typically permits 70 min. of observing per flight at altitudes in excess of 45,000 ft (13 km). Telescope system installation is discussed, along with appropriate setup and adjustment procedures. Operation of the guidance system is also explained, and checklists are provided which pertain to the recommended safe operating and in-flight trouble-shooting procedures for the equipment.

  19. Boosted Jets at the LHC

    NASA Astrophysics Data System (ADS)

    Larkoski, Andrew

    2015-04-01

    Jets are collimated streams of high-energy particles ubiquitous at any particle collider experiment and serve as proxy for the production of elementary particles at short distances. As the Large Hadron Collider at CERN continues to extend its reach to ever higher energies and luminosities, an increasingly important aspect of any particle physics analysis is the study and identification of jets, electroweak bosons, and top quarks with large Lorentz boosts. In addition to providing a unique insight into potential new physics at the tera-electron volt energy scale, high energy jets are a sensitive probe of emergent phenomena within the Standard Model of particle physics and can teach us an enormous amount about quantum chromodynamics itself. Jet physics is also invaluable for lower-level experimental issues including triggering and background reduction. It is especially important for the removal of pile-up, which is radiation produced by secondary proton collisions that contaminates every hard proton collision event in the ATLAS and CMS experiments at the Large Hadron Collider. In this talk, I will review the myriad ways that jets and jet physics are being exploited at the Large Hadron Collider. This will include a historical discussion of jet algorithms and the requirements that these algorithms must satisfy to be well-defined theoretical objects. I will review how jets are used in searches for new physics and ways in which the substructure of jets is being utilized for discriminating backgrounds from both Standard Model and potential new physics signals. Finally, I will discuss how jets are broadening our knowledge of quantum chromodynamics and how particular measurements performed on jets manifest the universal dynamics of weakly-coupled conformal field theories.

  20. Pharmacology of a cloned potassium channel from mouse brain (MK-1) expressed in CHO cells: effects of blockers and an 'inactivation peptide'.

    PubMed Central

    Robertson, B.; Owen, D. G.

    1993-01-01

    1. Chinese hamster ovary cells (CHO), maintained in cell culture, were stably transfected with DNA for the MK-1 voltage-activated potassium channel, previously cloned from a mouse brain library. 2. Voltage-activated currents were recorded by the whole cell patch clamp method. In CHO cells transfected with the vector only, there were no significant outward voltage activated currents. However, large outward voltage-activated potassium currents were always observed in those cells which had been transfected with the vector containing the DNA encoding for MK-1. 3. These potassium currents activated from -40 mV, and reversed at the potassium equilibrium potential. The half-maximal conductance of MK-1 was at -10 mV and had a slope factor of 11 mV when fitted with a Boltzmann function. There was only very slight (< 10%) inactivation of MK-1 even at very large positive voltages. 4. MK-1 was reversibly blocked by: 4-aminopyridine (4-AP, 0.1-4 mM), Toxin I 10-100 nM), mast cell degranulating peptide (1 microM), tetraethylammonium (TEA, 4-10 mM), tedisamil (100 microM), quinine (100 microM) and ciclazindol (100 microM); all applied to the outside of the cell from a 'U tube' rapid perfusion system. 4-AP may block closed as well as open MK-1 potassium channels. 5. A synthetic 20 amino acid peptide derived from the N-terminus sequence of the Shaker B potassium channel (the 'inactivation peptide') produced dramatic inactivation of MK-1 when applied to the inside, but not the outside of the cell. Reducing peptide concentration or 'degrading' the peptide produced less inactivation. 6. The block of MK-1 by the synthetic inactivation peptide was quite different in time dependence from block by internal TEA (0.4-4 mM), which probably blocks much more quickly but less potently than the peptide. PMID:8358568

  1. [18F]MK-9470, a positron emission tomography (PET) tracer for in vivo human PET brain imaging of the cannabinoid-1 receptor

    PubMed Central

    Burns, H. Donald; Van Laere, Koen; Sanabria-Bohórquez, Sandra; Hamill, Terence G.; Bormans, Guy; Eng, Wai-si; Gibson, Ray; Ryan, Christine; Connolly, Brett; Patel, Shil; Krause, Stephen; Vanko, Amy; Van Hecken, Anne; Dupont, Patrick; De Lepeleire, Inge; Rothenberg, Paul; Stoch, S. Aubrey; Cote, Josee; Hagmann, William K.; Jewell, James P.; Lin, Linus S.; Liu, Ping; Goulet, Mark T.; Gottesdiener, Keith; Wagner, John A.; de Hoon, Jan; Mortelmans, Luc; Fong, Tung M.; Hargreaves, Richard J.

    2007-01-01

    [18F]MK-9470 is a selective, high-affinity, inverse agonist (human IC50, 0.7 nM) for the cannabinoid CB1 receptor (CB1R) that has been developed for use in human brain imaging. Autoradiographic studies in rhesus monkey brain showed that [18F]MK-9470 binding is aligned with the reported distribution of CB1 receptors with high specific binding in the cerebral cortex, cerebellum, caudate/putamen, globus pallidus, substantia nigra, and hippocampus. Positron emission tomography (PET) imaging studies in rhesus monkeys showed high brain uptake and a distribution pattern generally consistent with that seen in the autoradiographic studies. Uptake was blocked by pretreatment with a potent CB1 inverse agonist, MK-0364. The ratio of total to nonspecific binding in putamen was 4–5:1, indicative of a strong specific signal that was confirmed to be reversible via displacement studies with MK-0364. Baseline PET imaging studies in human research subject demonstrated behavior of [18F]MK-9470 very similar to that seen in monkeys, with very good test–retest variability (7%). Proof of concept studies in healthy young male human subjects showed that MK-0364, given orally, produced a dose-related reduction in [18F]MK-9470 binding reflecting CB1R receptor occupancy by the drug. Thus, [18F]MK-9470 has the potential to be a valuable, noninvasive research tool for the in vivo study of CB1R biology and pharmacology in a variety of neuropsychiatric disorders in humans. In addition, it allows demonstration of target engagement and noninvasive dose-occupancy studies to aid in dose selection for clinical trials of CB1R inverse agonists. PMID:17535893

  2. In vivo analysis of insulin-like growth factor type 1 receptor humanized monoclonal antibody MK-0646 and small molecule kinase inhibitor OSI-906 in colorectal cancer.

    PubMed

    Leiphrakpam, Premila D; Agarwal, Ekta; Mathiesen, Michelle; Haferbier, Katie L; Brattain, Michael G; Chowdhury, Sanjib

    2014-01-01

    The development and characterization of effective anticancer drugs against colorectal cancer (CRC) is of urgent need since it is the second most common cause of cancer death. The study was designed to evaluate the effects of two IGF-1R antagonists, MK-0646, a recombinant fully humanized monoclonal antibody and OSI-906, a small molecule tyrosine kinase inhibitor on CRC cells. Xenograft study was performed on IGF-1R-dependent CRC cell lines for analyzing the antitumor activity of MK-0646 and OSI-906. Tumor proliferation and apoptosis were assessed using Ki67 and TUNEL assays, respectively. We also performed in vitro characterization of MK-0646 and OSI-906 treatment on CRC cells to identify mechanisms associated with drug-induced cell death. Exposure of the GEO and CBS tumor xenografts to MK-0646 or OSI-906 led to a decrease in tumor growth. TUNEL analysis showed an increase of approximately 45-55% in apoptotic cells in both MK-0646 and OSI-906 treated tumor samples. We report the novel finding that treatment with IGF-1R antagonists led to downregulation of X-linked inhibitor of apoptosis (XIAP) protein involved in cell survival and inhibition of cell death. In conclusion, IGF-1R antagonists (MK-0646 and OSI-906) demonstrated single agent inhibition of subcutaneous CRC xenograft growth. This was coupled to pro-apoptotic effects resulting in downregulation of XIAP and inhibition of cell survival. We report a novel mechanism by which MK-0646 and OSI-906 elicits cell death in vivo and in vitro. Moreover, these results indicate that MK-0646 and OSI-906 may be potential anticancer candidates for the treatment of patients with IGF-1R-dependent CRC. PMID:24173770

  3. Stress-dependent phosphorylation of myocardin-related transcription factor A (MRTF-A) by the p38MAPK/MK2 axis

    PubMed Central

    Ronkina, Natalia; Lafera, Juri; Kotlyarov, Alexey; Gaestel, Matthias

    2016-01-01

    Myocardin-related transcription factor A (MRTF-A) is a known actin-regulated transcriptional coactivator of serum response factor (SRF). Stimulation of actin polymerization activates MRTF-A by releasing it from G-actin and thus allowing it to bind to and activate SRF. Here, we compared protein phosphorylation in MK2/3-deficient cells rescued or not by ectopic expression of MK2 in two independent phosphoproteomic approaches using anisomycin-treated MEF cells and LPS-stimulated mouse macrophages, respectively. Two MRTF-A sites, Ser351 (corresponding to Ser312 in human) and Ser371 (Ser333 in human), showed significantly stronger phosphorylation (12-fold and 6-fold increase) in the cells expressing MK2. MRTF-A is phosphorylated at these sites in a stress-, but not in a mitogen-induced manner, and p38MAPK/MK2 catalytic activities are indispensable for this phosphorylation. MK2-mediated phosphorylation of MRTF-A at Ser312 and Ser333 was further confirmed in an in vitro kinase assay and using the phospho-protein kinase-D (PKD)-consensus motif antibody (anti-LXRXXpS/pT), the p38MAPK inhibitor BIRB-796, MK2/3-deficient cells and MRTF-A phospho-site mutants. Unexpectedly, dimerization, subcellular localization and translocation, interaction with actin, SRF or SMAD3 and transactivating potential of MRTF-A seem to be unaffected by manipulating the p38MAPK/MK2-dependent phosphorylations. Hence, MRTF-A is stress-dependently phosphorylated by MK2 at Ser312 and Ser333 with so far undetected functional and physiological consequences. PMID:27492266

  4. Measurement of the Top Pair Production Cross Section in the Lepton + Jets Channel Using a Jet Flavor Discriminant

    SciTech Connect

    Aaltonen, T.

    2011-08-01

    We present a new method to measure the top quark pair production cross section and the background rates with data corresponding to an integrated luminosity of 2.7 fb-1 from pp¯ collisions at √s = 1.96 TeV collected with the CDF II Detector. We select events with a single electron or muon candidate, missing transverse energy, and at least one b-tagged jet. We perform a simultaneous fit to a jet flavor discriminant across nine samples defined by the number of jets and b-tags. An advantage of this approach is that many systematic uncertainties are measured in situ and inversely scale with integrated luminosity. We measure a top cross section of σtt¯ = 7.64 ± 0.57 (stat + syst) ± 0.45 (luminosity) pb.

  5. Measurement of the Top Pair Production Cross Section in the Lepton + Jets Channel Using a Jet Flavor Discriminant

    DOE PAGESBeta

    Aaltonen, T.

    2011-08-01

    We present a new method to measure the top quark pair production cross section and the background rates with data corresponding to an integrated luminosity of 2.7 fb-1 from pp¯ collisions at √s = 1.96 TeV collected with the CDF II Detector. We select events with a single electron or muon candidate, missing transverse energy, and at least one b-tagged jet. We perform a simultaneous fit to a jet flavor discriminant across nine samples defined by the number of jets and b-tags. An advantage of this approach is that many systematic uncertainties are measured in situ and inversely scale withmore » integrated luminosity. We measure a top cross section of σtt¯ = 7.64 ± 0.57 (stat + syst) ± 0.45 (luminosity) pb.« less

  6. In Vitro Activities of Ertapenem (MK-0826) against Recent Clinical Bacteria Collected in Europe and Australia

    PubMed Central

    Livermore, David M.; Carter, Michael W.; Bagel, Simone; Wiedemann, Bernd; Baquero, Fernando; Loza, Elena; Endtz, Hubert P.; van den Braak, Nicole; Fernandes, Clarence J.; Fernandes, Lorna; Frimodt-Moller, Niels; Rasmussen, Laura S.; Giamarellou, Helen; Giamarellos-Bourboulis, Evangelos; Jarlier, Vincent; Nguyen, Jacqueline; Nord, Carl-Erik; Struelens, Marc J.; Nonhoff, Caire; Turnidge, John; Bell, Jan; Zbinden, Reinhard; Pfister, Stefan; Mixson, Lori; Shungu, Daniel L.

    2001-01-01

    Ertapenem (MK-0826, L-749,345) is a 1-β-methyl carbapenem with a long serum half-life. Its in vitro activity was determined by broth microdilution against 3,478 bacteria from 12 centers in Europe and Australia, with imipenem, cefepime, ceftriaxone, and piperacillin-tazobactam used as comparators. Ertapenem was the most active agent tested against members of the family Enterobacteriaceae, with MICs at which 90% of isolates are inhibited (MIC90s) of ≤1 μg/ml for all species. Ertapenem also was more active than imipenem against fastidious gram-negative bacteria and Moraxella spp.; on the other hand, ertapenem was slightly less active than imipenem against streptococci, methicillin-susceptible staphylococci, and anaerobes, but its MIC90s for these groups remained ≤0.5 μg/ml. Acinetobacter spp. and Pseudomonas aeruginosa were also much less susceptible to ertapenem than imipenem, and most Enterococcus faecalis strains were resistant. Ertapenem resistance, based on a provisional NCCLS MIC breakpoint of ≥16 μg/ml, was seen in only 3 of 1,611 strains of the family Enterobacteriaceae tested, all of them Enterobacter aerogenes. Resistance was also seen in 2 of 135 anaerobes, comprising 1 Bacteroides fragilis strain and 1 Clostridium difficile strain. Ertapenem breakpoints for streptococci have not been established, but an unofficial susceptibility breakpoint of ≤2 μg/ml was adopted for clinical trials to generate corresponding clinical response data for isolates for which MICs were as high as 2 μg/ml. Of 234 Streptococcus pneumoniae strains tested, 2 required ertapenem MICs of 2 μg/ml and one required an MIC of 4 μg/ml, among 67 non-Streptococcus pyogenes, non-Streptococcus pneumoniae streptococci, single isolates required ertapenem MICs of 2 and 16 μg/ml. These streptococci also had diminished susceptibilities to other β-lactams, including imipenem as well as ertapenem. The Etest and disk diffusion gave susceptibility test results in good agreement with

  7. Treadmill exercise inhibits hippocampal apoptosis through enhancing N-methyl-D-aspartate receptor expression in the MK-801-induced schizophrenic mice

    PubMed Central

    Chung, Jin Woo; Seo, Jin-Hee; Baek, Sang-Bin; Kim, Chang-Ju; Kim, Tae-Woon

    2014-01-01

    Schizophrenia is a severe mental disorder characterized by abnormal mental functioning and disruptive behaviors. Abnormal expression of N-methyl-D-aspartate (NMDA) receptor, one of the glutamate receptor subtypes, has also been suggested to contribute to the symptoms of schizophrenia. The effect of treadmill exercise on schizophrenia-induced apoptosis in relation with NMDA receptor has not been evaluated. In the present study, we investigated the effect of treadmill exercise on neuronal apoptosis in the hippocampus using MK-801-induced schizophrenic mice. MK-801 was intraperitoneally injected once a day for 2 weeks. The mice in the exercise groups were forced to run on a treadmill exercise for 60 min, once a day for 2 weeks. In the present results, repeated injection of the NMDA receptor antagonist MK-801 reduced expression of NMDA receptor in hippocampal CA2-3 regions. MK-801 injection increased casapse-3 expression and enhanced cytochrome c release in the hippocampus. The ratio of Bax to Bcl-2 was higher in the MK-801-induced schizophrenia mice than the normal mice. In contrast, treadmill exercise enhanced NMDA receptor expression, suppressed caspae-3 activation and cytochrome c release, and inhibited the ratio of Bax to Bcl-2. Based on present finding, we concluded that NMDA receptor hypofunctioning induced neuronal apoptosis in MK-801-induced schizophrenic mice. Treadmill exercise suppressed neuronal apoptosis through enhancing NMDA receptor expression in schizophrenic mice. PMID:25210696

  8. Exploring resistance mechanisms of HCV NS3/4A protease mutations to MK5172: insight from molecular dynamics simulations and free energy calculations.

    PubMed

    Guan, Yan; Sun, Huiyong; Pan, Peichen; Li, Youyong; Li, Dan; Hou, Tingjun

    2015-09-01

    Mutations at a number of key positions (Ala156, Asp168 and Arg155) of the HCV NS3/4A protease can induce medium to high resistance to MK5172. The emergence of the resistant mutations seriously compromises the antiviral therapy efficacy to hepatitis C. In this study, molecular dynamics (MD) simulations, free energy calculations and free energy decomposition were used to explore the interaction profiles of MK5172 with the wild-type (WT) and four mutated (R155K, D168A, D168V and A156T) HCV NS3/4A proteases. The binding free energies predicted by Molecular Mechanics/Generalized Born Solvent Area (MM/GBSA) are consistent with the trend of the experimental drug resistance data. The free energy decomposition analysis shows that the resistant mutants may change the protein-MK5172 interaction profiles, resulting in the unbalanced energetic distribution amongst the catalytic triad, the strand 135-139 and the strand 154-160. Moreover, umbrella sampling (US) simulations were employed to elucidate the unbinding processes of MK5172 from the active pockets of the WT HCV NS3/4A protease and the four mutants. The US simulations demonstrate that the dissociation pathways of MK5172 escaping from the binding pockets of the WT and mutants are quite different, and it is quite possible that MK5172 will be harder to get access to the correct binding sites of the mutated proteases, thereafter leading to drug resistance. PMID:26219385

  9. The combination of glutamate receptor antagonist MK-801 with tamoxifen and its active metabolites potentiates their antiproliferative activity in mouse melanoma K1735-M2 cells.

    PubMed

    Ribeiro, Mariana P C; Nunes-Correia, Isabel; Santos, Armanda E; Custódio, José B A

    2014-02-15

    Recent reports suggest that N-methyl-d-aspartate receptor (NMDAR) blockade by MK-801 decreases tumor growth. Thus, we investigated whether other ionotropic glutamate receptor (iGluR) antagonists were also able to modulate the proliferation of melanoma cells. On the other hand, the antiestrogen tamoxifen (TAM) decreases the proliferation of melanoma cells, and is included in combined therapies for melanoma. As the efficacy of TAM is limited by its metabolism, we investigated the effects of the NMDAR antagonist MK-801 in combination with TAM and its active metabolites, 4-hydroxytamoxifen (OHTAM) and endoxifen (EDX). The NMDAR blockers MK-801 and memantine decreased mouse melanoma K1735-M2 cell proliferation. In contrast, the NMDAR competitive antagonist APV and the AMPA and kainate receptor antagonist NBQX did not affect cell proliferation, suggesting that among the iGluR antagonists only the NMDAR channel blockers inhibit melanoma cell proliferation. The combination of antiestrogens with MK-801 potentiated their individual effects on cell biomass due to diminished cell proliferation, since it decreased the cell number and DNA synthesis without increasing cell death. Importantly, TAM metabolites combined with MK-801 promoted cell cycle arrest in G1. Therefore, the data obtained suggest that the activity of MK-801 and antiestrogens in K1735-M2 cells is greatly enhanced when used in combination. PMID:24240127

  10. MK615 attenuates Porphyromonas gingivalis lipopolysaccharide-induced pro-inflammatory cytokine release via MAPK inactivation in murine macrophage-like RAW264.7 cells.

    PubMed

    Morimoto, Yoko; Kikuchi, Kiyoshi; Ito, Takashi; Tokuda, Masayuki; Matsuyama, Takashi; Noma, Satoshi; Hashiguchi, Teruto; Torii, Mitsuo; Maruyama, Ikuro; Kawahara, Ko-Ichi

    2009-11-01

    The Japanese apricot, known as Ume in Japanese, has been a traditional Japanese medicine for centuries, and is a familiar and commonly consumed food. The health benefits of Ume are now being widely recognized and have been strengthened by recent studies showing that MK615, an extract of compounds from Ume, has strong anticancer and anti-inflammatory effects. However, the potential role of MK615 in the periodontal field remains unknown. Here, we found that MK615 significantly reduced the production of pro-inflammatory mediators (tumor necrosis factor-alpha and interleukin-6) induced by Porphyromonas gingivalis lipopolysaccharide (LPS), a major etiological agent in localized chronic periodontitis, in murine macrophage-like RAW264.7 cells. MK615 markedly inhibited the phosphorylation of ERK1/2, p38MAPK, and JNK, which is associated with pro-inflammatory mediator release pathways. Moreover, MK615 completely blocked LPS-triggered NF-kappaB activation. The present results suggest that MK615 has potential as a therapeutic agent for treating inflammatory diseases such as periodontitis. PMID:19706286

  11. Magnetic Untwisting in Solar Jets that Go into the Outer Corona in Polar Coronal Holes

    NASA Technical Reports Server (NTRS)

    Moore, Ronald L.; Sterling, Alphonse C.; Falconer, David A.

    2014-01-01

    We present results from 14 exceptionally high-reaching large solar jets observed in the polar coronal holes. EUV movies from SDO/AIA show that each jet is similar to many other similar-size and smaller jets that erupt in coronal holes, but each is exceptional in that it goes higher than most other jets, so high that it is observed in the outer corona beyond 2.2 R(sub Sun) in images from the SOHO/LASCO/C2 coronagraph. For these high-reaching jets, we find: (1) the front of the jet transits the corona below 2.2 R(sub Sun) at a speed typically several times the sound speed; (2) each jet displays an exceptionally large amount of spin as it erupts; (3) in the outer corona, most jets display oscillatory swaying having an amplitude of a few degrees and a period of order 1 hour. We conclude that these jets are magnetically driven, propose that the driver is a magnetic-untwisting wave that is grossly a large-amplitude (i.e., nonlinear) torsional Alfven wave that is put into the reconnected open magnetic field in the jet by interchange reconnection as the jet erupts, and estimate from the measured spinning and swaying that the magnetic-untwisting wave loses most of its energy in the inner corona below 2.2 R(sub Sun). From these results for these big jets, we reason that the torsional magnetic waves observed in Type-II spicules should dissipate in the corona in the same way and could thereby power much of the coronal heating in coronal holes.

  12. The Highest Redshift Relativistic Jets

    SciTech Connect

    Cheung, C.C.; Stawarz, L.; Siemiginowska, A.; Harris, D.E; Schwartz, D.A.; Wardle, J.F.C.; Gobeille, D.; Lee, N.P.

    2007-12-18

    We describe our efforts to understand large-scale (10's-100's kpc) relativistic jet systems through observations of the highest-redshift quasars. Results from a VLA survey search for radio jets in {approx} 30 z > 3.4 quasars are described along with new Chandra observations of 4 selected targets.

  13. Calorimeter Optimization for Jet Identification

    SciTech Connect

    Bower, G.

    2004-10-11

    During LCWS 1999 at Sitges, Spain, we presented a set of discriminators for reconstructing jets in high energy lepton colliders. We have extended that study by adding new event types, by adding new discriminators, and by using a neural net rather than cuts to identify jets. We apply these techniques to detector simulations to begin a study of calorimeter optimization.

  14. Magnetic Field Topology in Jets

    NASA Technical Reports Server (NTRS)

    Gardiner, T. A.; Frank, A.

    2000-01-01

    We present results on the magnetic field topology in a pulsed radiative. jet. For initially helical magnetic fields and periodic velocity variations, we find that the magnetic field alternates along the, length of the jet from toroidally dominated in the knots to possibly poloidally dominated in the intervening regions.

  15. Nonlinear Dynamics in Viscoelastic Jets

    NASA Astrophysics Data System (ADS)

    Majmudar, Trushant; Varagnat, Matthieu; McKinley, Gareth

    2009-03-01

    Instabilities in free surface continuous jets of non-Newtonian fluids, although relevant for many industrial processes, remain poorly understood in terms of fundamental fluid dynamics. Inviscid, and viscous Newtonian jets have been studied in considerable detail, both theoretically and experimentally. Instability in viscous jets leads to regular periodic coiling of the jet, which exhibits a non-trivial frequency dependence with the height of the fall. Here we present a systematic study of the effect of viscoelasticity on the dynamics of continuous jets of worm-like micellar surfactant solutions of varying viscosities and elasticities. We observe complex nonlinear spatio-temporal dynamics of the jet, and uncover a transition from periodic to quasi-periodic to a multi-frequency, broad-spectrum dynamics. Beyond this regime, the jet dynamics smoothly crosses over to exhibit the ``leaping shampoo'' or the Kaye effect. We examine different dynamical regimes in terms of scaling variables, which depend on the geometry (dimensionless height), kinematics (dimensionless flow rate), and the fluid properties (elasto-gravity number) and present a regime map of the dynamics of the jet in terms of these dimensionless variables.

  16. Nonlinear Dynamics in Viscoelastic Jets

    NASA Astrophysics Data System (ADS)

    Majmudar, Trushant; Varagnat, Matthieu; McKinley, Gareth

    2008-11-01

    Instabilities in free surface continuous jets of non-Newtonian fluids, although relevant for many industrial processes, remain poorly understood in terms of fundamental fluid dynamics. Inviscid, and viscous Newtonian jets have been studied in considerable detail, both theoretically and experimentally. Instability in viscous jets leads to regular periodic coiling of the jet, which exhibits a non-trivial frequency dependence with the height of the fall. Here we present a systematic study of the effect of viscoelasticity on the dynamics of continuous jets of worm-like micellar surfactant solutions of varying viscosities and elasticities. We observe complex nonlinear spatio-temporal dynamics of the jet, and uncover a transition from periodic to quasi-periodic to a multi-frequency, broad-spectrum dynamics. Beyond this regime, the jet dynamics smoothly crosses over to exhibit the ``leaping shampoo'' or the Kaye effect. We examine different dynamical regimes in terms of scaling variables, which depend on the geometry (dimensionless height), kinematics (dimensionless flow rate), and the fluid properties (elasto-gravity number) and present a regime map of the dynamics of the jet in terms of these dimensionless variables.

  17. OPENING ANGLES OF COLLAPSAR JETS

    SciTech Connect

    Mizuta, Akira; Ioka, Kunihito

    2013-11-10

    We investigate the jet propagation and breakout from the stellar progenitor for gamma-ray burst (GRB) collapsars by performing two-dimensional relativistic hydrodynamic simulations and analytical modeling. We find that the jet opening angle is given by θ{sub j} ∼ 1/5Γ{sub 0} and infer the initial Lorentz factor of the jet at the central engine, Γ{sub 0}, is a few for existing observations of θ{sub j}. The jet keeps the Lorentz factor low inside the star by converging cylindrically via collimation shocks under the cocoon pressure and accelerates at jet breakout before the free expansion to a hollow-cone structure. In this new picture, the GRB duration is determined by the sound crossing time of the cocoon, after which the opening angle widens, reducing the apparent luminosity. Some bursts violating the maximum opening angle θ{sub j,{sub max}} ∼ 1/5 ∼ 12° imply the existence of a baryon-rich sheath or a long-acting jet. We can explain the slopes in both Amati and Yonetoku spectral relations using an off-centered photosphere model, if we make only one assumption that the total jet luminosity is proportional to the initial Lorentz factor of the jet. We also numerically calibrate the pre-breakout model (Bromberg et al.) for later use.

  18. Jets on comet Borrelly

    NASA Technical Reports Server (NTRS)

    2001-01-01

    This very long exposure was taken by NASA's Deep Space 1 to show detailed structures in the faint parts of comet Borrelly's inner coma. As a result, the nucleus has been greatly over-exposed and its shape appears distorted. The main jet extends to the lower right about 30 degrees off the Sun-line. Faint structures in the coma stretch from the nucleus in all directions. The surface of Borrelly is composed of a mixture of dust and water ice, and as the comet approaches the Sun the ice sublimes. Dust carried outward by expanding gas makes the jets visible.

    Deep Space 1 completed its primary mission testing ion propulsion and 11 other advanced, high-risk technologies in September 1999. NASA extended the mission, taking advantage of the ion propulsion and other systems to undertake this chancy but exciting, and ultimately successful, encounter with the comet. More information can be found on the Deep Space 1 home page at http://nmp.jpl.nasa.gov/ds1/ .

    Deep Space 1 was launched in October 1998 as part of NASA's New Millennium Program, which is managed by JPL for NASA's Office of Space Science, Washington, D.C. The California Institute of Technology manages JPL for NASA.

  19. Consolidating NASA's Arc Jets

    NASA Technical Reports Server (NTRS)

    Balboni, John A.; Gokcen, Tahir; Hui, Frank C. L.; Graube, Peter; Morrissey, Patricia; Lewis, Ronald

    2015-01-01

    The paper describes the consolidation of NASA's high powered arc-jet testing at a single location. The existing plasma arc-jet wind tunnels located at the Johnson Space Center were relocated to Ames Research Center while maintaining NASA's technical capability to ground-test thermal protection system materials under simulated atmospheric entry convective heating. The testing conditions at JSC were reproduced and successfully demonstrated at ARC through close collaboration between the two centers. New equipment was installed at Ames to provide test gases of pure nitrogen mixed with pure oxygen, and for future nitrogen-carbon dioxide mixtures. A new control system was custom designed, installed and tested. Tests demonstrated the capability of the 10 MW constricted-segmented arc heater at Ames meets the requirements of the major customer, NASA's Orion program. Solutions from an advanced computational fluid dynamics code were used to aid in characterizing the properties of the plasma stream and the surface environment on the calorimeters in the supersonic flow stream produced by the arc heater.

  20. Dense Hypervelocity Plasma Jets

    NASA Astrophysics Data System (ADS)

    Case, Andrew; Witherspoon, F. Douglas; Messer, Sarah; Bomgardner, Richard; Phillips, Michael; van Doren, David; Elton, Raymond; Uzun-Kaymak, Ilker

    2007-11-01

    We are developing high velocity dense plasma jets for fusion and HEDP applications. Traditional coaxial plasma accelerators suffer from the blow-by instability which limits the mass accelerated to high velocity. In the current design blow-by is delayed by a combination of electrode shaping and use of a tailored plasma armature created by injection of a high density plasma at a few eV generated by arrays of capillary discharges or sparkgaps. Experimental data will be presented for a complete 32 injector gun system built for driving rotation in the Maryland MCX experiment, including data on penetration of the plasma jet through a magnetic field. We present spectroscopic measurements of plasma velocity, temperature, and density, as well as total momentum measured using a ballistic pendulum. Measurements are in agreement with each other and with time of flight data from photodiodes and a multichannel PMT. Plasma density is above 10^15 cm-3, velocities range up to about 100 km/s. Preliminary results from a quadrature heterodyne HeNe interferometer are consistent with these results.

  1. THE EVOLUTION OF HETERGENEOUS 'CLUMPY JETS': A PARAMETER STUDY

    SciTech Connect

    Yirak, Kristopher; Schroeder, Ed; Frank, Adam; Cunningham, Andrew J.

    2012-02-20

    We investigate the role discrete clumps embedded in an astrophysical jet play on the jet's morphology and line emission characteristics. By varying clumps' size, density, position, and velocity, we cover a range of parameter space motivated by observations of objects such as the Herbig-Haro object HH 34. We here extend the results presented in Yirak et al., including how analysis of individual observations may lead to spurious sinusoidal variation whose parameters vary widely over time, owing chiefly to interactions between clumps. The goodness of fits, while poor in all simulations, are best when clump-clump collisions are minimal. Our results indicate that a large velocity dispersion leads to a clump-clump collision-dominated flow which disrupts the jet beam. Finally, we present synthetic emission images of H{alpha} and [S II] and note an excess of [S II] emission along the jet length as compared to observations. This suggests that observed beams undergo earlier processing, if they are present at all.

  2. Background density channel generation by axial plasma jets

    NASA Astrophysics Data System (ADS)

    Bonde, Jeffrey; Vincena, Stephen; Gekelman, Walter

    2012-10-01

    The supersonic expansion of a dense plasma into an ambient plasma can be observed in phenomena ranging from coronal mass ejections and protostellar outflows to astrophysical jets. To produce a supersonic plasma jet in a laboratory setting, a laser-produced plasma explodes into an ambient argon plasma (n˜5.10^12cm-3,cs˜6.10^5cm/s,vA˜1.2.10^7cm/s) in the Large Plasma Device at UCLA. This study focuses on the initial formation and evolution of the jet and its effects on the background magnetized plasma. Using a laser-induced fluorescence diagnostic of Ar-II ions at their 611.5nm transition, the jet is seen to perturb the equilibrium population of the target argon ions. A CCD camera with a fast (>=3ns) shutter spatially and temporally resolves images of the fluorescence. Time-lapsed imaging shows an axially aligned channel of depleted fluorescence form near the source and travel with an undiminished speed characteristic of the jet (v/cs˜20) while remaining highly collimated. Langmuir probe measurements show a large ion flux moving in conjunction with the excited argon depletion after traveling more than an ion inertial length.

  3. Observations of Multiple Blobs in Homologous Solar Coronal Jets in Closed Loop

    NASA Astrophysics Data System (ADS)

    Zhang, Q. M.; Ji, H. S.; Su, Y. N.

    2016-03-01

    Coronal bright points (CBPs) and jets are ubiquitous small-scale brightenings that are often associated with each other. We here report our multiwavelength observations of two groups of homologous jets. The first group was observed by the Extreme-Ultraviolet Imager (EUVI) onboard the Solar TErrestrial RElations Observatory (STEREO) Behind spacecraft in 171 Å and 304 Å on 2014 September 10, from a location where no data from the Solar Dynamic Observatory (SDO) could be obtained. The jets (J1 - J6) recurred for six times with intervals of 5 - 15 minutes. They originated from the same primary CBP (BP1) and propagated in the northeast direction along large-scale, closed coronal loops. Two of the jets (J3 and J6) produced sympathetic CBPs (BP2 and BP3) after reaching the remote footpoints of the loops. The time delays between the peak times of BP1 and BP2 (BP3) are 240±75 s (300±75 s). The jets were not coherent. Instead, they were composed of bright and compact blobs. The sizes and apparent velocities of the blobs are 4.5 - 9 Mm and 140 - 380 km s-1. The arrival times of the multiple blobs in the jets at the far end of the loops indicate that the sympathetic CBPs are caused by jet flows and not by thermal conduction fronts. The second group was observed by the Atmospheric Imaging Assembly onboard SDO in various wavelengths on 2010 August 3. Similar to the first group, the jets originated from a short-lived BP at the boundary of Active Region 11092 and propagated along a small-scale, closed loop before flowing into the active region. Several tiny blobs with sizes of ˜1.7 Mm and an apparent velocity of ˜238 km s^{-1} were identified in the jets. We carried out differential emission measure (DEM) inversions to investigate the temperatures of the blobs, finding that the blobs were multithermal with an average temperature of 1.8 - 3.1 MK. The estimated number densities of the blobs were (1.7 - 2.8)×109 cm^{-3}.

  4. Magnetically driven jets and winds

    NASA Technical Reports Server (NTRS)

    Lovelace, R. V. E.; Berk, H. L.; Contopoulos, J.

    1991-01-01

    Four equations for the origin and propagation of nonrelativistic jets and winds are derived from the basic conservation laws of ideal MHD. The axial current density is negative in the vicinity of the axis and positive at larger radii; there is no net current because this is energetically favored. The magnetic field is essential for the jet solutions in that the zz-component of the magnetic stress acts, in opposition to gravity, to drive matter through the slow magnetosonic critical point. For a representative self-consistent disk/jet solution relevant to a protostellar system, the reaction of the accreted mass expelled in the jets is 0.1, the ratio of the power carried by the jets to the disk luminosity is 0.66, and the ratio of the boundary layer to disk luminosities is less than about 0.13. The star's rotation rate decreases with time even for rotation rates much less than the breakup rate.

  5. Dynamic Lyα jets

    NASA Astrophysics Data System (ADS)

    Koza, J.; Rutten, R. J.; Vourlidas, A.

    2009-06-01

    Context: The solar chromosphere and transition region are highly structured and complex regimes. A recent breakthrough has been the identification of dynamic fibrils observed in Hα as caused by field-aligned magnetoacoustic shocks. Aims: We seek to find whether such dynamic fibrils are also observed in Lyα. Methods: We used a brief sequence of four high-resolution Lyα images of the solar limb taken by the Very high Angular resolution ULtraviolet Telescope (VAULT), which displays many extending and retracting Lyα jets. We measured their top trajectories and fitted parabolas to the 30 best-defined ones. Results: Most jet tops move supersonically. Half of them decelerate, sometimes superballistically, the others accelerate. This bifurcation may arise from incomplete sampling of recurrent jets. Conclusions: The similarities between dynamic Lyα jets and Hα fibrils suggest that the magnetoacoustic shocks causing dynamic Hα fibrils also affect dynamic Lyα jets.

  6. Computational Modeling And Analysis Of Synthetic Jets

    NASA Technical Reports Server (NTRS)

    Mittal, Rajat; Cattafesta, Lou

    2005-01-01

    In the last report we focused on the study of 3D synthetic jets of moderate jet aspect-ratio. Jets in quiescent and cross-flow cases were investigated. Since most of the synthetic jets in practical applications are found to be of large aspect ratio, the focus was shifted to studying synthetic jets of large aspect ratio. In the current year, further progress has been made by studying jets of aspect ratio 8 and infinity. Some other aspects of the jet, like the vorticity flux is looked into apart from analyzing the vortex dynamics, velocity profiles and the other dynamical characteristics of the jet which allows us to extract some insight into the effect of these modifications on the jet performance. Also, efforts were made to qualitatively validate the simulated results with the NASA Langley test cases at higher jet Reynolds number for the quiescent jet case.

  7. Tracing Carina's protostellar jets to the source with WFC3-IR

    NASA Astrophysics Data System (ADS)

    Smith, Nathan

    2010-09-01

    Based on a recent H-alpha imaging survey of the Carina Nebula with ACS, we discovered 39 new Herbig-Haro {HH} jets marking the bipolar outflows from newly formed low-mass stars. This provides a valuable sample of jets that are all at the same distance, allowing us to study the relationship between protostars and their outflows where nearby massive stars are shredding the molecular cloud as new stars are forming. Carina has been studied extensively with Spitzer and Chandra, providing IR spectral energy distributions and X-ray luminosities for all detectable sources in the field. However, 29 of the jets seen at visual wavelengths emerge from opaque clouds or dark globules seen in silhouette against the bright background H II region, so the protostellar driving sources are still embedded in their natal clouds and are not identifiable in optical images. This makes the identification of the driving sources in lower resolution Spitzer and Chandra images ambiguous. Three of these jets {HH666, HH901, and HH902} have already been imaged with WFC3 as ERO targets after the servicing mission, demonstrating that near-IR [Fe II] emission lines in the F126N and F164N filters provide an excellent way to trace the jet emission back into the cloud and to thereby identify which embedded star is driving the outflow. Here we propose to obtain WFC3-IR images of [Fe II] emission from the remaining embedded jets in order to link the jets to the protostars that launch them. This will then permit a comparison of the jet properties to protostar properties for the full sample. Additionally, the flux ratio of these two [Fe II] lines will probe the spatially dependent extinction through the cloud all the way back to the source, providing a map of the density structure in the protostar's extended circumstellar envelope.

  8. ASPHERICAL CORE-COLLAPSE SUPERNOVAE IN RED SUPERGIANTS POWERED BY NONRELATIVISTIC JETS

    SciTech Connect

    Couch, Sean M.; Wheeler, J. Craig; Milosavljevic, Milos

    2009-05-01

    We explore the observational characteristics of jet-driven supernovae (SNe) by simulating bipolar-jet-driven explosions in a red supergiant (RSG) progenitor. We present results of four models in which we hold the injected kinetic energy at a constant 10{sup 51} erg across all jet models but vary the specific characteristics of the jets to explore the influence of the nature of jets on the structure of the SN ejecta. We evolve the explosions past shock-breakout and into quasi-homologous expansion of the SN envelope into a RSG wind. The simulations have sufficient numerical resolution to study the stability of the flow. Our simulations show the development of fluid instabilities that produce pristine helium clumps in the hydrogen envelope. The oppositely directed, nickel-rich jets give a large-scale asymmetry that may account for the nonspherical excitation and substructure of spectral lines such as H{alpha} and He I 10830 A. Jets with a large fraction of kinetic to thermal energy punch through the progenitor envelope and give rise to explosions that would be observed to be asymmetric from the earliest epochs, inconsistent with spectropolarimetric measurements of Type II SNe. Jets with higher thermal energy fractions result in explosions that are roughly spherical at large radii but are significantly elongated at smaller radii, deep inside the ejecta, in agreement with the polarimetric observations. We present shock-breakout light curves that indicate that strongly aspherical shock breakouts are incompatible with recent Galaxy Evolution Explorer observations of shock breakout from RSG stars. Comparison with observations indicates that jets must deposit their kinetic energy efficiently throughout the ejecta while in the hydrogen envelope. Thermal-energy-dominated jets satisfy this criterion and yield many of the observational characteristics of Type II SNe.

  9. Hemiasterlin derivative (R)(S)(S)-BF65 and Akt inhibitor MK-2206 synergistically inhibit SKOV3 ovarian cancer cell growth.

    PubMed

    Lai, Wei-Ting; Cheng, Kai-Lin; Baruchello, Riccardo; Rondanin, Riccardo; Marchetti, Paolo; Simoni, Daniele; Lee, Ray M; Guh, Jih-Hwa; Hsu, Lih-Ching

    2016-08-01

    We reported previously that a hemiasterlin derivative BF65 is a potent anticancer agent that can inhibit microtubule assembly. Here we show that a more potent stereospecific diastereomer (R)(S)(S)-BF65 can synergize with an allosteric Akt inhibitor MK-2206 to suppress the growth of SKOV3 ovarian cancer cells with constitutively active Akt. (R)(S)(S)-BF65 induced mitotic arrest and MK-2206 caused G0/G1 arrest, while the combination of both induced simultaneous G0/G1 and G2/M cell cycle arrest. (R)(S)(S)-BF65 induced phosphorylation and inactivation of Bcl-2, and downregulated Mcl-1, consequently may lead to apoptosis. (R)(S)(S)-BF65 inhibited mitogen-activated protein kinases (MAPKs), which may stimulate cell proliferation upon activation. (R)(S)(S)-BF65 also induced DNA damage after long-term treatment. MK-2206 is known to inhibit phosphorylation and activation of Akt and suppress cancer cell growth. The combination of (R)(S)(S)-BF65 and MK-2206 also inhibited the Akt pathway. Interestingly, MK-2206 upregulated Bcl-2 and induced activation of MAPKs in SKOV3 cells; however, when combined with (R)(S)(S)-BF65, these prosurvival effects were reversed. The combination also more significantly decreased Mcl-1 protein, increased PARP cleavage, and induced γ-H2AX, a DNA damage marker. Remarkably, MK-2206 enhanced the microtubule depolymerization effect of (R)(S)(S)-BF65. The combination of (R)(S)(S)-BF65 and MK-2206 also markedly inhibited cell migration. Thus, MK-2206 synergizes with (R)(S)(S)-BF65 to inhibit SKOV3 cell growth via downregulating the Akt signaling pathway, and enhancing the microtubule disruption effect of (R)(S)(S)-BF65. (R)(S)(S)-BF65 in turn suppresses Bcl-2 and MAPKs induced by MK-2206. (R)(S)(S)-BF65 and MK-2206 compensate each other leading to increased apoptosis and enhanced cytotoxicity, and may also suppress cancer cell invasion. PMID:27328368

  10. Collimation and Stability of Three Dimensional Jets

    NASA Astrophysics Data System (ADS)

    Hardee, P. E.; Clarke, D. A.; Howell, D. A.

    1993-12-01

    Three-dimensional numerical simulations of cylindrical jets established in equilibrium with a surrounding uniform medium have been performed. Large scale structures such as helical twisting of the jet, elliptical distortion and bifurcation of the jet, and triangular distortion and trifurcation of the jet have been seen in the simulations. The grid resolution has been sufficient to allow the development of structures on smaller scales and has revealed higher order distortions of the jet surface and complex structure internal to the jet. However, smaller scale surface distortion and internal jet structure do not significantly modify the large scale dynamics. It is the large scale surface distortions and accompanying filamentation that dominate the jet dynamics. Decollimation occurs as the jet bifurcates or trifurcates. Jets with density less than the immediately surrounding medium rapidly decollimate and expand as the jet filaments into multiple streams leading to shock heating and mass entrainment. The resulting morphology resembles a turbulent plume and might be relevant to some FRI type radio sources. Jet densities higher than the immediately surrounding medium are required to produce FRII type radio source jet morphology and protostellar jet morphology. Thus, while jets may be denser or lighter than the external medium through which they propagate, it is the conditions in the cocoon or lobe around the jet that governs the dynamics far behind the jet front. This work was supported by NSF grant AST-8919180, EPSCoR grant EHR-9108761 and NSF-REU grant AST-9300413.

  11. A Phase I Trial Of MK-2206 In Children with Refractory Malignancies: A Children's Oncology Group Study

    PubMed Central

    Fouladi, Maryam; Perentesis, John P.; Phillips, Christine L.; Leary, Sarah; Reid, Joel M.; McGovern, Renee M.; Ingle, Ashish M.; Ahern, Charlotte H.; Ames, Matthew M.; Houghton, Peter; Doyle, L. Austin; Weigel, Brenda; Blaney, Susan M.

    2015-01-01

    Background We report results of a phase I trial designed to estimate the maximum tolerated dose (MTD), describe dose-limiting toxicities (DLT), and characterize the pharmacokinetic profile of MK-2206, an AKT inhibitor, in children with refractory or recurrent malignancies. Procedure MK-2206 was administered either every other day (schedule 1), or once a week (Schedule 2) in a 28-day cycle. Dose escalations in increments of ∼30% were independently made in each part using the rolling-six design. Serial pharmacokinetic (PK) studies were obtained. Biological studies include analysis of PI3K/PTEN/AKT-cell signaling pathway in pre and post-therapy in PBMC and in tumors at diagnosis or recurrence. Results Fifty patients [26 males, median age 12.6 years (range, 3.1-21.9)] with malignant glioma (16), ependymoma (4), hepatocellular carcinoma (3), gliomatosis cereberi (2) or other tumors (22) were enrolled; 40 were fully evaluable for toxicity (schedule 1 n=23; schedule 2 n=17). Schedule 1 DLTs included: grade 3 dehydration in 1/6 patients at 28 mg/m2; grade 4 hyperglycemia and neutropenia in 1/6 patients at 45 mg/ m2; and grade 3 rash in 3/6 patients at dose level 4 (58 mg/m2). Schedule 2 DLTs included: grade 3 alkaline phosphatase in 1/6 patients at 90 mg/m2; grade 3 rash in 1/6 patients at 120 mg/ m2, and grade 3 rash in 2/6 patients at 155 mg/m2. Conclusions The recommended pediatric phase 2 dose of MK-2206 is 45 mg/m2/dose every other day or 120 mg/m2/dose weekly. Pharmacokinetics appeared linear over the dose range studied. PMID:24664955

  12. Effects of several cerebroprotective drugs on NMDA channel function: evaluation using Xenopus oocytes and [3H]MK-801 binding.

    PubMed

    Kaneko, S; Sugimura, M; Inoue, T; Satoh, M

    1991-06-19

    The effects of several cerebroprotective and nootropic drugs on the function of excitatory amino acid (EAA) receptor subtypes expressed in Xenopus oocytes after injection of rodent brain poly(A)+ mRNA were investigated. The oocyte response to N-methyl-D-aspartate (NMDA) in the presence of glycine (Gly) was inhibited dose-dependently by bifemelane, indeloxazine, vinpocetine and vincamine while no effect was observed by idebenone, Ca hopantenate, aniracetam or piracetam. Bifemelane, indeloxazine and vinpocetine suppressed the maximum response of NMDA and Gly without affecting their EC50 values. Unlike Mg2+, they did not affect the current-voltage relationship of the NMDA response below 0 mV. On the non-NMDA-type responses of the injected oocytes to kainate (KA), alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) and quisqualate (QA), no significant effects were observed by these drugs at 100 microM. On the binding of [3H](+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imi ne (MK-801) to brain membranes, the estimated IC50 values were 88 microM for bifemelane, 102 microM for indeloxazine, and 115 microM for vinpocetine. The dissociation rate of [3H]MK-801 was significantly slowed by Zn2+ and vinpocetine, but not affected by bifemelane or indeloxazine. The Kd value for [3H]MK-801 binding was increased by bifemelane and indeloxazine while Bmax was unchanged. These results suggest that the inhibition of NMDA channels by vinpocetine shows a similarity to the action of Zn2+ which closes the gate of the NMDA channel. In contrast, bifemelane and indeloxazine may affect the phencyclidine (PCP)-site in the open channels and inhibit NMDA function. PMID:1652446

  13. Results on QCD Physics from the CDF-II Experiment

    SciTech Connect

    Pagliarone, C.; /Cassino U. /INFN, Pisa

    2006-12-01

    In this paper the authors review a selection of recent results obtained, in the area of QCD physics, from the CDF-II experiment that studies p{bar p} collisions at {radical}s = 1.96 TeV provided by the Fermilab Tevatron Collider. All results shown correspond to analysis performed using the Tevatron Run II data samples. In particular they will illustrate the progress achieved and the status of the studies on the following QCD processes: jet inclusive production, using different jet clustering algorithm, W({yields} e{nu}{sub e}) + jets and Z({yields} e{sup +}e{sup -}) + jets production, {gamma} + b-jet production, dijet production in double pomeron exchange and finally exclusive e{sup +}e{sup -} and {gamma}{gamma} production. No deviations from the Standard Model have been observed so far.

  14. Multiple jet study data correlations. [data correlation for jet mixing flow of air jets

    NASA Technical Reports Server (NTRS)

    Walker, R. E.; Eberhardt, R. G.

    1975-01-01

    Correlations are presented which allow determination of penetration and mixing of multiple cold air jets injected normal to a ducted subsonic heated primary air stream. Correlations were obtained over jet-to-primary stream momentum flux ratios of 6 to 60 for locations from 1 to 30 jet diameters downstream of the injection plane. The range of geometric and operating variables makes the correlations relevant to gas turbine combustors. Correlations were obtained for the mixing efficiency between jets and primary stream using an energy exchange parameter. Also jet centerplane velocity and temperature trajectories were correlated and centerplane dimensionless temperature distributions defined. An assumption of a Gaussian vertical temperature distribution at all stations is shown to result in a reasonable temperature field model. Data are presented which allow comparison of predicted and measured values over the range of conditions specified above.

  15. Measurement of the top quark mass in lepton+jets events with secondary vertex tagging

    SciTech Connect

    Harrington, Robert Duane; /Northeastern U.

    2007-02-01

    A measurement of the top quark mass with the matrix element method in the lepton + jets final state in D0 Run II is presented. Events with single isolated energetic charged lepton (electron or muon), exactly four calorimeter jets, and significant missing transverse energy are selected. Probabilities used to discriminate between signal and background are assumed to be proportional to differential cross-sections, calculated using event kinematics and folding in object resolutions and parton distribution functions. The event likelihoods constructed using these probabilities are varied with the top quark mass, m{sub t}, and the jet energy scale, JES, to give the smallest possible combined statistical + JES uncertainty.

  16. Dissociable effects of the noncompetitive NMDA receptor antagonists ketamine and MK-801 on intracranial self-stimulation in rats

    PubMed Central

    Hillhouse, Todd M.; Porter, Joseph H.; Negus, S. Stevens

    2014-01-01

    Rationale The noncompetitive NMDA antagonist ketamine produces rapid antidepressant effects in treatment-resistant patients suffering from major depressive and bipolar disorders. However, abuse liability is a concern. Objectives This study examined abuse-related effects of keta-mine using intracranial self-stimulation (ICSS) in rats. The higher-affinity NMDA antagonist MK-801 and the monoamine reuptake inhibitor cocaine were examined for comparison. Methods Male Sprague Dawley rats were implanted with electrodes targeting the medial forebrain bundle and trained to respond to brain stimulation under a frequency–rate ICSS procedure. The first experiment compared the potency and time course of ketamine (3.2–10.0 mg/kg) and MK-801 (0.032–0.32 mg/kg). The second experiment examined effects of repeated dosing with ketamine (3.2–20.0 mg/kg/day) and acute cocaine (10.0 mg/kg). Results Following acute administration, ketamine (3.2–10 mg/kg) produced only dose- and time-dependent depressions of ICSS and failed to produce an abuse-related facilitation of ICSS at any dose or pretreatment time. In contrast, MK-801 (0.032–0.32 mg/kg) produced a mixed profile of rate-increasing and rate-decreasing effects; ICSS facilitation was especially prominent at an intermediate dose of 0.18 mg/kg. Repeated dosing with ketamine produced dose-dependent tolerance to the rate-decreasing effects of ketamine (10.0 and 18.0 mg/kg) but failed to unmask expression of ICSS facilitation. Termination of ketamine treatment failed to produce withdrawal-associated decreases in ICSS. As reported previously, 10.0 mg/kg cocaine facilitated ICSS. Conclusions The dissociable effects of ketamine and MK-801 suggest differences in the pharmacology of these nominally similar NMDA antagonists. Failure of ketamine to facilitate ICSS contrasts with other evidence for the abuse liability of ketamine. PMID:24522331

  17. Fermionic T-duality in massive type IIA supergravity on AdS_{10-k} × M_k

    NASA Astrophysics Data System (ADS)

    Bakhmatov, Ilya

    2016-04-01

    Fermionic T-duality transformation is studied for supersymmetric solutions of massive type IIA supergravity with the metric AdS_{10-k} × M_k for k=3 and 5. We derive the Killing spinors of these backgrounds and use them as input for the fermionic T-duality transformation. The resulting dual solutions form a large family of supersymmetric deformations of the original solutions by complex valued RR fluxes. We observe that the Romans mass parameter does not change under fermionic T-duaity, and prove its invariance in the k=3 case.

  18. Novel ATP competitive MK2 inhibitors with potent biochemical and cell-based activity throughout the series.

    PubMed

    Oubrie, Arthur; Kaptein, Allard; de Zwart, Edwin; Hoogenboom, Niels; Goorden, Rianne; van de Kar, Bas; van Hoek, Maaike; de Kimpe, Vera; van der Heijden, Ruud; Borsboom, Judith; Kazemier, Bert; de Roos, Jeroen; Scheffers, Michiel; Lommerse, Jos; Schultz-Fademrecht, Carsten; Barf, Tjeerd

    2012-01-01

    Optimization of our previously described pyrrolopiperidone series led to the identification of a new benzamide sub-series, which exhibits consistently high potency in biochemical and cell-based assays throughout the series. Strong inhibition of LPS-induced production of the cytokine TNFα is coupled to the regulation of HSP27 phosphorylation, indicating that the observed cellular effects result from the inhibition of MK2. X-ray crystallographic and computational analyses provide a rationale for the high potency of the series. PMID:22119462

  19. Bifurcation and exact traveling wave solutions of a modified nonlinearly dispersive mK (m,n,k) equation

    NASA Astrophysics Data System (ADS)

    Zhang, Lijun; Chen, Li-Qun; Zhang, Jianming

    2013-10-01

    Bifurcation and exact solutions of the modified nonlinearly dispersive mK (m,n,k) equation with nonlinear dispersion um-1ut+a(un)x+b(uk)xxx = 0,nk≠0 are investigated in this paper. As a result, under different parameter conditions, abundant compactons, peakons and solitary solutions including not only some known results but also some new ones are obtained. We also point out the original reason of the existence of the non-smooth traveling wave solutions. The approach we used here is also suitable for the study of traveling wave solutions of some other nonlinear equations.

  20. Experimental 'jet lag' causes sympathoexcitation via oxidative stress through AT1 receptor in the brainstem.

    PubMed

    Kishi, T; Sunagawa, K

    2011-01-01

    Circadian disruptions through frequent transmeridian travel, rotating shift work, and poor sleep hygiene are associated with an array of physical and mental health maladies, including the abnormal autonomic nervous system. We have demonstrated that the oxidative stress through AT(1) receptor in the brain activates sympathetic nervous system. The aim of the present study was to determine whether experimental 'jet lag' causes sympathoexcitation via oxidative stress through AT(1) receptor in the cardiovascular center of the brainstem (rostral ventrolateral medulla; RVLM) or not. Experimental 'jet lag' was made to normotensive (Wister-Kyoto rat; WKY rat) and hypertensive rats (stroke-prone spontaneously hypertensive rats; SHRSP) by the exposure to a 12 hour phase advance for 5 days. In WKY, 'jet lag' increases blood pressure and the activity of sympathetic nervous system via oxidative stress through angiotensin II type 1 receptor in the RVLM for 2 days only, and the changes are improved at 3 day after the initiation of 'jet lag'. In SHRSP, 'jet lag' also increases blood pressure and the activity of sympathetic nervous system via oxidative stress through angiotensin II type 1 receptor in the RVLM, and the changes are greater compared to those in WKY, and are maintained for the period of 'jet lag'. These results suggest that experimental 'jet lag' causes sympathoexcitation via oxidative stress through AT(1) receptor in the brain, especially in hypertension. PMID:22254719