Sample records for kalli ktuse tttu
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1951-06-09
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RADC Thermal Guide for Reliability Engineers.
1982-06-01
sis in government procurements has been to tailor the (e.g., cockpit or crew area) environmental definition to the equipment’s intended use, thereby...AD- AIIB 839 HUGHES AIRCRAFT CO CULVER CITY CA F/G 20/13 ,RAUC THERMAL GUIDE FOR RELIABILITY ENSINEERS (U)’ JUN 82 6 N MORRISON. J N KALLIS. L A...Yet many a reliability engineer has little or no knowledge of thermal design and analysis. Moreover, texts and government handbooks published to
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CD8 T Cells and Immunoediting of Breast Cancer
2008-08-01
Reiman , C.J. Krco, I.T. Lamborn, and K.L. Knutson. CD8 T cells promote relapse of breast cancer by inducing epithelial to mesenchymal transition. J...Immunother 2006;29:682 (Abstract). 8 Santisteban, M., J.M. Reiman , M.K. Asiedu, M.D. Behrens, A. Nassar, K.R. Kalli, P. Haluska, J.N. Ingle, L.C...immune mechanisms, and their association with prognostic indicators. Surgery 2003;134(5):827-34. 3. Behrens, M.D., J. Reiman , C.J. Krco, I.T. Lamborn
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Voltage-sensing phosphatase modulation by a C2 domain.
Castle, Paul M; Zolman, Kevin D; Kohout, Susy C
2015-01-01
The voltage-sensing phosphatase (VSP) is the first example of an enzyme controlled by changes in membrane potential. VSP has four distinct regions: the transmembrane voltage-sensing domain (VSD), the inter-domain linker, the cytosolic catalytic domain, and the C2 domain. The VSD transmits the changes in membrane potential through the inter-domain linker activating the catalytic domain which then dephosphorylates phosphatidylinositol phosphate (PIP) lipids. The role of the C2, however, has not been established. In this study, we explore two possible roles for the C2: catalysis and membrane-binding. The Ci-VSP crystal structures show that the C2 residue Y522 lines the active site suggesting a contribution to catalysis. When we mutated Y522 to phenylalanine, we found a shift in the voltage dependence of activity. This suggests hydrogen bonding as a mechanism of action. Going one step further, when we deleted the entire C2 domain, we found voltage-dependent enzyme activity was no longer detectable. This result clearly indicates the entire C2 is necessary for catalysis as well as for modulating activity. As C2s are known membrane-binding domains, we tested whether the VSP C2 interacts with the membrane. We probed a cluster of four positively charged residues lining the top of the C2 and suggested by previous studies to interact with phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] (Kalli et al., 2014). Neutralizing those positive charges significantly shifted the voltage dependence of activity to higher voltages. We tested membrane binding by depleting PI(4,5)P2 from the membrane using the 5HT2C receptor and found that the VSD motions as measured by voltage clamp fluorometry (VCF) were not changed. These results suggest that if the C2 domain interacts with the membrane to influence VSP function it may not occur exclusively through PI(4,5)P2. Together, this data advances our understanding of the VSP C2 by demonstrating a necessary and critical role for the C2 domain in
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Voltage-sensing phosphatase modulation by a C2 domain
Castle, Paul M.; Zolman, Kevin D.; Kohout, Susy C.
2015-01-01
The voltage-sensing phosphatase (VSP) is the first example of an enzyme controlled by changes in membrane potential. VSP has four distinct regions: the transmembrane voltage-sensing domain (VSD), the inter-domain linker, the cytosolic catalytic domain, and the C2 domain. The VSD transmits the changes in membrane potential through the inter-domain linker activating the catalytic domain which then dephosphorylates phosphatidylinositol phosphate (PIP) lipids. The role of the C2, however, has not been established. In this study, we explore two possible roles for the C2: catalysis and membrane-binding. The Ci-VSP crystal structures show that the C2 residue Y522 lines the active site suggesting a contribution to catalysis. When we mutated Y522 to phenylalanine, we found a shift in the voltage dependence of activity. This suggests hydrogen bonding as a mechanism of action. Going one step further, when we deleted the entire C2 domain, we found voltage-dependent enzyme activity was no longer detectable. This result clearly indicates the entire C2 is necessary for catalysis as well as for modulating activity. As C2s are known membrane-binding domains, we tested whether the VSP C2 interacts with the membrane. We probed a cluster of four positively charged residues lining the top of the C2 and suggested by previous studies to interact with phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] (Kalli et al., 2014). Neutralizing those positive charges significantly shifted the voltage dependence of activity to higher voltages. We tested membrane binding by depleting PI(4,5)P2 from the membrane using the 5HT2C receptor and found that the VSD motions as measured by voltage clamp fluorometry (VCF) were not changed. These results suggest that if the C2 domain interacts with the membrane to influence VSP function it may not occur exclusively through PI(4,5)P2. Together, this data advances our understanding of the VSP C2 by demonstrating a necessary and critical role for the C2 domain in
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Effects of solar dimming and brightening on the terrestrial carbon sink
NASA Astrophysics Data System (ADS)
Mercado, L.; Bellouin, N.; Sitch, S.; Boucher, O.; Huntingford, C.; Wild, M.; Cox, P. M.
2009-04-01
photosynthesis for climate model applications. Tellus Series B-Chemical and Physical Meteorology, 59, 553-565. Niyogi D., Chang H.I., Saxena V.K., Holt T., Alapaty K., Booker F., Chen F., Davis K.J., Holben B., Matsui T., Meyers T., Oechel W.C., Pielke R.A., Wells R., Wilson K. & Xue Y.K. (2004) Direct observations of the effects of aerosol loading on net ecosystem CO2 exchanges over different landscapes. Geophysical Research Letters, 31. Oliveira P.H.F., Artaxo P., Pires C., De Lucca S., Procopio A., Holben B., Schafer J., Cardoso L.F., Wofsy S.C. & Rocha H.R. (2007) The effects of biomass burning aerosols and clouds on the CO2 flux in Amazonia. Tellus Series B-Chemical and Physical Meteorology, 59, 338-349. Roderick M.L., Farquhar G.D., Berry S.L. & Noble I.R. (2001) On the direct effect of clouds and atmospheric particles on the productivity and structure of vegetation. Oecologia, 129, 21-30. Stanhill G. & Cohen S. (2001) Global dimming: a review of the evidence for a widespread and significant reduction in global radiation with discussion of its probable causes and possible agricultural consequences. 107, 255-278. Wild M., Gilgen H., Roesch A., Ohmura A., Long C.N., Dutton E.G., Forgan B., Kallis A., Russak V. & Tsvetkov A. (2005) From dimming to brightening: Decadal changes in solar radiation at Earth's surface. Science, 308, 847-850.
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EDITORIAL: Optical Fibre Sensors 18 (OFS-18)
NASA Astrophysics Data System (ADS)
Jones, Julian D. C.; Tatam, Ralph P.
2007-10-01
fibre optic gyroscope, conventionally taken to date from the first experimental demonstration by V Vali and R W Shorthill (1976 'Fibre ring interferometer' Appl. Opt. 15 1099-100). It is an indication of the health of the community that the successful maturity of some applications is complemented by the new technologies that will be the basis of the future development of the field, and here the content of this special issue is an interesting indication of likely areas of growth. Essentially all current fibre optic systems are based on solid, doped fused silica fibres, which are the basis of the world's telecommunications industry. However, over the last decade an exciting development has been micro-structured fibres, whose waveguiding properties owe as much to the structure of the fibre as to the materials from which they are made. The significance for sensing applications is considerable, with opportunities to achieve properties for dispersion, environmental sensitivity, wavelength range and power-handling quite different from the capabilities of conventional fibre. Thus it is not surprising that several of the papers in the issue (by Cordeiro, Martynkien, Bock, Wolinski, Michie, Digonnet and Kilic) are devoted to applications of such fibres—photonic crystal fibres (PCF), as they are often called. Digonnet's contribution is especially interesting, being concerned with the use of hollow-core photonic bandgap fibre to form a gyroscope, hence avoiding the many subtle non-linear optical effects that can degrade the performance of a conventional fibre gyroscope. PCF are not the only special fibres to feature in the issue: polymer fibres are of increasing interest for sensing applications (O'Keeffe, Kiesel, Kalli and Ashley), not least for their ability to withstand high levels of strain. In-fibre gratings continue to be a very important area in the field, and are well represented in the issue (Ni, González-Segura, Chen, Falate, Kamikawachi, Wang and Correia). We hope
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Hydrologic regime alteration of a Mediterranean catchment under climate change projection
NASA Astrophysics Data System (ADS)
Sellami, Haykel; Benabdallah, Sihem; La Jeunesse, Isabelle; Herrmann, Frank; Vanclooster, Marnik
2014-05-01
. López-Moreno, J. I., Vicente-Serrano, S. M., Moran-Tejeda, E., Zabalza, J., Lorenzo-Lacruz, J., and García-Ruiz, J. M.: Impact of climate evolution and land use changes on water yield in the ebro basin, Hydrology and Earth System Sciences, 15, 311-322, 10.5194/hess-15-311-2011, 2011. Ludwig, R., Roson, R., Zografos, C., Kallis. Towards an inter-disciplinary research agenda on climate change, water and security in southern Europe and neighbouring countries. Environ. Sci. Policy. 14: 794-803, 10.1016/j.envsci.2011.04.003, 2011. Richter, B. D., Baumgartner, J.V., Powell, J., Braun, D. P.: Method for Assessing Hydrologic Alteration within Ecosystems, Conservation Biology, 10, 11, 1996. Schneider, C., Laizé, C. L. R., Acreman, M. C., and Flörke, M.: How will climate change modify river flow regimes in Europe? Hydrology and Earth System Sciences, 17, 325-339, 10.5194/hess-17-325-2013, 2013.