Science.gov

Sample records for konsa mirja ots

  1. MASTER: bright OTs

    NASA Astrophysics Data System (ADS)

    Gress, O.; Vladimirov, V.; Lipunov, V.; Gorbovskoy, E.; Buckley, D.; Balanutsa, P.; Tiurina, N.; Kornilov, V.; Kuznetsov, A.; Gorbunov, I.; Vlasenko, D.; Kuvshinov, D.; Popova, E.; Potter, S.; Kotze, M.; Budnev, N.; Poleschuk, V.; Ivanov, K.

    2016-01-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 14h 50m 56.15s -25d 55m 26.9s on 2016-01-27.02773 UT. The OT unfiltered magnitude is 16.3 (limit 18.4m).

  2. Master: 3 OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Buckley, D.; Tlatov, A.; Gorbovskoy, E.; Tyurina, N.; Kuznetsov, A.; Kornilov, V.; Kuvshinov, D.; Popova, E.; Vlasenko, D.; Shumkov, V.; Potter, S.; Kniazev, A.; Budnev, N.; Gress, O.; Ivanov, K.; Senik, V.; Dormidontov, D.; Parhomenko, A. V.

    2016-08-01

    MASTER-Kislovodsk auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 21h 33m 50.58s +06d 51m 22.5s on 2016-07-27.94690 UT. The OT unfiltered magnitude is 17.8m (limit 18.0m).

  3. MASTER-Kislovodsk: bright high amplitude OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Gorbovskoy, E.; Tiurina, N.; Kornilov, V.; Gress, O.; Kuznetsov, A.; Gorbunov, I.; Vlasenko, D.; Vladimirov, V.; Tlatov, A.; Senik, V.; Dormidontov, D.; Parkhomenko, A.

    2016-02-01

    MASTER-Kislovodsk auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 08h 41m 40.94s +72d 27m 32.6s on 2016-02-22.89248 UT. The OT unfiltered magnitude is . The OT is seen in 3 images.

  4. MASTER: 5.2mag amplitude OT

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Lipunov, V.; Buckley, D.; Potter, S.; Kniazev, A.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kornilov, V.; Kuznetsov, A.; Vladimirov, V.; Gress, O.; Vlasenko, D.; Gorbunov, I.; Kuvshinov, D.; Shumkov, V.

    2016-03-01

    MASTER OT J203533.66-260924.0 discovery - possible dwarf nova outburst, Ampl > 5.2mag MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 20h 35m 33.66s -26d 09m 24.0s on 2016-03-12.11795 UT. The OT unfiltered magnitude is 16.8m (the limit is 19.0m).

  5. MASTER: PSN in PGC135878 and OTs

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Shumkov, V.; Lipunov, V.; Buckley, D.; Lopez, R. Rebolo; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Gorbunov, I.; Gress, O.; Ricart, M. Serra; Israelian, G.; Potter, S.; Kniazev, A.

    2016-06-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 18h 02m 41.87s +09d 03m 12.9s on 2016-06-05.16160 UT. The OT unfiltered magnitude is (limit 19.1m).The OT is seen in 10 images.

  6. Two novel splice variants of SOX2OT, SOX2OT-S1, and SOX2OT-S2 are coupregulated with SOX2 and OCT4 in esophageal squamous cell carcinoma.

    PubMed

    Shahryari, Alireza; Rafiee, Mahmoud Reza; Fouani, Youssef; Oliae, Nasrin Alipour; Samaei, Nader Mansour; Shafiee, Mohammad; Semnani, Shahryar; Vasei, Mohammad; Mowla, Seyed Javad

    2014-01-01

    Long noncoding RNAs (lncRNAs) have emerged as new regulators of stem cell pluripotency and tumorigenesis. The SOX2 gene, a master regulator of pluripotency, is embedded within the third intron of a lncRNA known as SOX2 overlapping transcript (SOX2OT). SOX2OT has been suspected to participate in regulation of SOX2 expression and/or other related processes; nevertheless, its potential involvement in tumor initiation and/or progression is unclear. Here, we have evaluated a possible correlation between expression patterns of SOX2OT and those of master regulators of pluripotency, SOX2 and OCT4, in esophageal squamous cell carcinoma (ESCC) tissue samples. We have also examined its potential function in the human embryonic carcinoma stem cell line, NTERA2 (NT2), which highly expresses SOX2OT, SOX2, and OCT4. Our data revealed a significant coupregulation of SOX2OT along with SOX2 and OCT4 in tumor samples, compared to the non-tumor tissues obtained from the margin of same tumors. We also identified two novel splice variants of SOX2OT (SOX2OT-S1 and SOX2OT-S2) which coupregulated with SOX2 and OCT4 in ESCCs. Suppressing SOX2OT variants caused a profound alteration in cell cycle distribution, including a 5.9 and 6.9 time increase in sub-G1 phase of cell cycle for SOX2OT-S1 and SOX2OT-S2, respectively. The expression of all variants was significantly diminished, upon the induction of neural differentiation in NT2 cells, suggesting their potential functional links to the undifferentiated state of the cells. Our data suggest a part for SOX2OT spliced variants in tumor initiation and/or progression as well as regulating pluripotent state of stem cells. PMID:24105929

  7. MASTER-Net: PSN and 3 OTs

    NASA Astrophysics Data System (ADS)

    Gorbovskoy, E.; Balanutsa, P.; Lipunov, V.; Lopez, R. Rebolo; Ricart, M. Serra; Buckley, D.; Israelian, G.; Tiurina, N.; Kornilov, V.; Kuznetsov, A.; Gress, O.; Shumkov, V.; Gorbunov, I.; Vlasenko, D.; Vladimirov, V.; Popova, E.; Potter, S.; Kotze, M.; Tlatov, A.; Senik, V.; Dormidontov, D.; Parkhomenko, A.

    2016-02-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 16h 33m 55.46s +86d 08m 30.4s on 2016-02-13.88456 UT. The OT unfiltered magnitude is (limit 18.6m).

  8. MASTER-SAAO: bright outbursting OT

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Lipunov, V.; Buckley, D.; Kornilov, V.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Gress, O.; Kuznetsov, A.; Kuvshinov, D.; Vladimirov, V.; Gorbunov, I.; Vlasenko, D.; Popova, E.; Potter, S.; Kniazev, A.; Shurpakov, S.

    2016-04-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 21h 29m 20.71s -62d 08m 52.2s on 2016-04-27.985 UT. The OT unfiltered magnitude is 17.3m (the limit is 19.3m).

  9. Master-Iac Master-Saao OTs

    NASA Astrophysics Data System (ADS)

    Pogrosheva, T.; Vladimirov, V.; Lipunov, V.; Lopez, R. Rebolo; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Balanutsa, P.; Kornilov, V.; Gorbunov, I.; Gress, O.; Shumkov, V.; Ricart, M. Serra; Israelian, G.; Potter, S.; Kniazev, A.

    2016-06-01

    MASTER-IAC auto-detection system( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 20h 58m 16.98s +23d 58m 12.6s on 2016-06-09.03890 UT. The OT unfiltered magnitude is 17.5m (limit 19.4m).

  10. MASTER: PSN in PGC858421 and OTs

    NASA Astrophysics Data System (ADS)

    Tiurina, N.; Balanutsa, P.; Lipunov, E. Popova V.; Buckley, D.; Gorbovskoy, E.; Kuznetsov, A.; Kornilov, V.; Vlasenko, D.; Gress, O.; Shurpakov, S.; Potter, S.; Kniazev, A.

    2016-07-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 14h 47m 32.66s -19d 12m 42.1s on 2016-07-09.87385 UT. PSN unfiltered magnitude is (limit 19.8m).

  11. 75 FR 51169 - OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... Office of Thrift Supervision OTS Minority Depository Institutions Advisory Committee AGENCY: Department of the Treasury, Office of Thrift Supervision (OTS). ACTION: Notice. SUMMARY: The Charter for the OTS..., Office of Thrift Supervision, 1700 G Street, NW., Washington, DC 20552. SUPPLEMENTARY INFORMATION:...

  12. MASTER OT 015347+303844 discovery

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Gorbovskoy, E.; Lipunov, V.; Kornilov, V.; Belinski, A.; Shatskiy, N.; Tyurina, N.; Kuvshinov, D.; Chazov, V.; Kuznetsov, A.; Zimnukhov, D.; Kornilov, M.; Tlatov, A.; Parhomenko, A. V.; Dormidontov, D.; Yurkov, V.; Sergienko, Yu.; Varda, D.; Krushinski, V.; Zalozhnih, I.; Kopytova, T.; Popov, A.; Ivanov, K.; Yazev, S.; Budnev, N.; Konstantinov, E.; Lenok, V.; Chuvalaev, O.; Poleschuk, V.; Gres, O.

    2010-10-01

    MASTER auto-detection system at Kislovodsk detected a new transient source at the position (R.A., Dec) = 01h 53m 47.24s , +30d 38m 44s.0 (J2000) with a typical uncertainty of 0.2" at 2010-10-01 23:25:48 (UT). The V magnitude is about 17.9 +-0.1 ( exposition 180s, m_lim = 19.0 ). The OT is seen at next 7 images up 2010-10-12 in R and V. OT is blue: R-V=1.43 at 2010-10-01.9 . There is no minor planet and any object brighter 21 mag in DSS at this place.

  13. MASTER-Kislovodsk: bright OT with 7mag amplitude

    NASA Astrophysics Data System (ADS)

    Gress, O.; Lipunov, V.; Gorbovskoy, E.; Samus, N.; Tiurina, N.; Balanutsa, P.; Vladimirov, V.; Kuznetsov, A.; Kornilov, V.; Shumkov, V.; Gorbunov, I.; Vlasenko, D.; Kuvshinov, D.; Tlatov, A.; Senik, V.; Dormidontov, D.; Parkhomenko, A.

    2016-02-01

    MASTER-Kislovodsk auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010 )discovered OT source at (RA, Dec) = 07h 33m 25.52s +37d 37m 44.9s on 2016-02-24.90468 UT. The OT unfiltered magnitude is 15.1m (limit 18.0m).The OT is seen in 3 images.

  14. MASTER discoveries: dwarf novae, PSN, OT in Andromeda direction

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Gress, O.; Balanutsa, P.; Lipunov, V.; Rebolo Lopez, R.; Serra Ricart, M.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Ivanov, K.

    2016-09-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 00h 41m 26.22s +41d 43m 50.0s on 2016-09-02.12339 UT. The OT unfiltered magnitude is 19.7m, mlim=20.0 The OT is seen in 14 images.

  15. Very Bright OT detected by MASTER-SAAO

    NASA Astrophysics Data System (ADS)

    Gress, O.; Lipunov, V.; Gorbovskoy, E.; Buckley, D.; Budnev, N.; Tiurina, N.; Balanutsa, P.; Kornilov, V.; Kuznetsov, A.; Shumkov, V.; Gorbunov, I.; Vlasenko, D.; Vladimirov, V.; Popova, E.; Potter, S.; Kotze, M.

    2016-01-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 00h 38m 31.10s -64d 03m 13.7s on 2016-01-26.85306 UT. The OT unfiltered magnitude is 12.7m (limit 18.5m).

  16. 12 CFR 500.10 - The OTS or The Office.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true The OTS or The Office. 500.10 Section 500.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY AGENCY ORGANIZATION AND FUNCTIONS General Organization § 500.10 The OTS or The Office. The Office of Thrift Supervision (referred...

  17. MASTER: very bright OT in Large Magellanic cloud direction

    NASA Astrophysics Data System (ADS)

    Gorbovskoy, E.; Lipunov, V.; Buckley, D.; Tiurina, N.; Balanutsa, P.; Kornilov, V.; Gorbunov, I.; Kuznetsov, A.; Gress, O.; Vladimirov, V.; Popova, E.; Vlasenko, D.; Kuvshinov, D.; Potter, S.

    2016-05-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 05h 10m 32.58s -69d 21m 30.4s on 2016-05-10.72797 UT. The OT unfiltered magnitude is (limit 17.6m).

  18. MASTER: PSN in PGC032587 and new OTs

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Popova, E.; Lipunov, V.; Lopez, R. Rebolo; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Gorbunov, I.; Chazov, V.; Gress, O.; Ivanov, K.; Vladimirov, V.; Vlasenko, D.; Kuvshinov, D.; Serra-Ricart, M.; Israelyan, G.; Potter, S.; Kniazev, A.

    2016-06-01

    MASTER-IAC auto-detection system Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 discovered OT source at (RA, Dec) = 10h 51m 45.85s +55d 23m 12.4s on 2016-05-31.97997 UT. The OT unfiltered magnitude is 18.3 (limit 20.1m).

  19. Master-Iac Master-Saao 2 bright OTs

    NASA Astrophysics Data System (ADS)

    Gress, O.; Pogrosheva, T.; Lipunov, V.; Lopez, R. Rebolo; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Gorbunov, I.; Kuznetsov, A.; Popova, E.; Kuvshinov, D.; Serra-Ricart, M.; Israelian, G.; Potter, S.; Kniazev, A.

    2016-05-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 00h 10m 21.33s +73d 15m 16.5s on 2016-05-24.18141 UT. The OT unfiltered magnitude is 15.7m (limit 18.1m).

  20. MASTER-Net discoveries: PSN, new Blazar and OT

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Balanutsa, P.; Popova, E.; Lipunov, V.; Buckley, D.; Lopez, R. Rebolo; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Vlasenko, D.; Gorbunov, I.; Gress, O.; Vladimirov, V.; Kuvshinov, D.; Ricart, M. Serra; Israelyan, G.; Potter, S.; Kniazev, A.

    2016-03-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 13h 57m 48.95s -27d 26m 23.4s on 2016-03-30.02359 UT. The OT unfiltered magnitude is 17.7m (limit 19.8m).

  1. MASTER-IAC: PSN in PGC1671957 and bright OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Rebolo Lopez, R.; Serra Ricart, M.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Gress, O.; Ivanov, K.; Shumkov, V.

    2016-09-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 23h 36m 08.12s +22d 32m 33.1s on 2016-09-07.05299 UT. The OT unfiltered magnitude is 17.8m (limit 20.1m).

  2. MASTER-IAC: bright QSO flare and OT discovery

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Popova, E.; Gress, O.; Lipunov, V.; Lopez, R. Rebolo; Serra-Ricart, M.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kornilov, V.; Kuznetsov, A.; Kuvshinov, D.; Vlasenko, D.; Chazov, V.; Israelyan, G.; Lodieu, N.; Budnev, N.; Ivanov, K.; Shurpakov, S.

    2016-07-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L )discovered OT source at (RA, Dec) = 19h 18m 40.69s +42d 21m 03.3s on 2016-07-25.00863 UT. The OT magnitude in unfiltered is 17.9m (limit 18.7m).

  3. MASTER-IAC: very bright high amplitude OT

    NASA Astrophysics Data System (ADS)

    Gress, O.; Lipunov, V.; Lopez, R. Rebolo; Serra-Ricart, M.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kornilov, V.; Kuznetsov, A.; Kuvshinov, D.; Vlasenko, D.; Vladimirov, V.; Popova, E.; Chazov, V.; Israelyan, G.; Lodieu, N.; Budnev, N.; Ivanov, K.

    2016-07-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 20h 48m 06.68s +13d 54m 23.3s on 2016-07-24.16661 UT. The OT unfiltered magnitude is (limit 18.5m).

  4. 5. SOUTHWEST PORTAL AND SOUTHWEST WEB OT THROUGH TRUSSES, SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. SOUTHWEST PORTAL AND SOUTHWEST WEB OT THROUGH TRUSSES, SHOWING TOP CHORD DETAILS; VIEW TO NORTHEAST - Nebraska City Bridge, Spanning Missouri River near Highway 2 between Nebraska & Iowa, Nebraska City, Otoe County, NE

  5. MASTER-SAAO and MATER-Net OTs detection

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Shumkov, V.; Lipunov, V. M.; Buckley, D. A. H.; Gorbovskoy, E.; Tiurina, N.; Shurpakov, S.; Gress, O.; Kuznetsov, A.; Vladimirov, V.; Kornilov, V.; Vlasenko, D.; Gorbunov, I.; Kniazev, A.; Potter, S. B.; Kotze, M.; Tlatov, A.; Senik, V.; Dormidontov, D.

    2016-03-01

    MASTER-SAAO auto-detection system (Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 07h 28m 58.62s -65d 21m 31.9s on 2016-02-28.87676 UT with unfiltered m_OT=19.0m (the limit is 20.3m).

  6. Blue MASTER OT J020844.29+481923.0 Detection

    NASA Astrophysics Data System (ADS)

    Vladimirov, V.; Zubareva, A.; Lipunov, V.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Pruzhinskaya, M.; Denisenko, D.; Rufanov, V.; Ivanov, K.; Yazev, S.; Budnev, N.; Gress, O.; Poleshchuk, V.; Parkhomenko, E. Konstantinov A.; Tlatov, A.; Dormidontov, D.; Yurkov, V. Senik V.; Sergienko, Y.; Varda, D.; Sinyakov, E.; Gabovich, V.; Krushinsky, V.; Zalozhnih, I.; Popov, A.; Bourdanov, A.; Buckley, D.; Potter, S.; Kniazev, A.; Kotze, M.; Shumkov, V.; Shurpakov, S.

    2015-01-01

    MASTER OT J020844.29+481923.0 - CV? MASTER-Tunka auto-detection system (Lipunov et al., 2010, Advances in Astronomy, vol. 2010, pp. 1, http://adsabs.harvard.edu/abs/2010AdAst2010E..30L) discovered OT source at (RA, Dec) = 02h 08m 44.29s +48d 19m 23.0s on 2015-01-08.52068 UT.

  7. MASTER: OT discovered during inspection of HESE 58537957 trigger

    NASA Astrophysics Data System (ADS)

    Tyurina, N.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Kuvshinov, D.; Vlasenko, D.; Gress, O.; Ivanov, K.; Shumkov, V.; Potter, S.

    2016-08-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 13h 08m 45.02s -32d 32m 54.9s on 2016-08-24.73811 UT during inspection of HESE alert ( 58537957 trigger number ) http://gcn.gsfc.nasa.gov/notices_amon/58537957_128340.amon . The OT unfiltered magnitude is 19.6m (limit 20.5m).

  8. MASTER: very bright high amplitude(>7.2m) OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, V.; Balanutsa, P.; Lipunov, V.; Lopez, R. Rebolo; Serra-Ricart, M.; Gorbovskoy, E.; Tiurina, N.; Kornilov, V.; Kuznetsov, A.; Kuvshinov, D.; Vlasenko, D.; Vladiirov, V.; Popova, E.; Chazov, V.; Israelyan, G.; Lodieu, N.; Budnev, N.; Gress, O.; Ivanov, K.

    2016-07-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 20h 53m 16.61s -10d 56m 33.1s on 2016-07-24.10765 UT. The OT unfiltered (W=0.2B+0.8R, calibrated by USNO-B1) magnitude is 14.8 (limit 18.7m).

  9. 12 CFR 502.50 - What fees does OTS charge?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true What fees does OTS charge? 502.50 Section 502.50... § 502.50 What fees does OTS charge? (a) The Director assesses fees for examining or investigating... U.S.C. 1462(9), except that, for this part only, “affiliate” does not include any entity that...

  10. OT promotes closer interpersonal distance among highly empathic individuals.

    PubMed

    Perry, Anat; Mankuta, David; Shamay-Tsoory, Simone G

    2015-01-01

    The space between people, or 'interpersonal distance', creates and defines the dynamics of social interactions and is a salient cue signaling responsiveness and feeling comfortable. This distance is implicit yet clearly felt, especially if someone stands closer or farther away than expected. Increasing evidence suggests that Oxytocin (OT) serves as a social hormone in humans, and that one of its roles may be to alter the perceptual salience of social cues. Considering that empathic ability may shape the way individuals process social stimuli, we predicted that OT will differentially affect preferred interpersonal distance depending on individual differences in empathy. Participants took part in two interpersonal distance experiments: In the first, they had to stop a (computer visualized) protagonist when feeling most comfortable; in the second, they were asked to choose the room in which they would later discuss intimate topics with another. Both experiments revealed an interaction between the effect of OT and empathy level. Among highly empathic individuals, OT promoted the choice of closer interpersonal distances. Yet, OT had an opposite effect on individuals with low empathic traits. We conclude that the enhancement of social cues following OT administration may have opposite effects on individuals with different empathic abilities. PMID:24509491

  11. OT promotes closer interpersonal distance among highly empathic individuals

    PubMed Central

    Mankuta, David; Shamay-Tsoory, Simone G.

    2015-01-01

    The space between people, or ‘interpersonal distance’, creates and defines the dynamics of social interactions and is a salient cue signaling responsiveness and feeling comfortable. This distance is implicit yet clearly felt, especially if someone stands closer or farther away than expected. Increasing evidence suggests that Oxytocin (OT) serves as a social hormone in humans, and that one of its roles may be to alter the perceptual salience of social cues. Considering that empathic ability may shape the way individuals process social stimuli, we predicted that OT will differentially affect preferred interpersonal distance depending on individual differences in empathy. Participants took part in two interpersonal distance experiments: In the first, they had to stop a (computer visualized) protagonist when feeling most comfortable; in the second, they were asked to choose the room in which they would later discuss intimate topics with another. Both experiments revealed an interaction between the effect of OT and empathy level. Among highly empathic individuals, OT promoted the choice of closer interpersonal distances. Yet, OT had an opposite effect on individuals with low empathic traits. We conclude that the enhancement of social cues following OT administration may have opposite effects on individuals with different empathic abilities. PMID:24509491

  12. A new assessment method of outdoor tobacco smoke (OTS) exposure

    NASA Astrophysics Data System (ADS)

    Cho, Hyeri; Lee, Kiyoung

    2014-04-01

    Outdoor tobacco smoke (OTS) is concerned due to potential health effects. An assessment method of OTS exposure is needed to determine effects of OTS and validate outdoor smoking policies. The objective of this study was to develop a new method to assess OTS exposure. This study was conducted at 100 bus stops including 50 centerline bus stops and 50 roadside bus stops in Seoul, Korea. Using real-time aerosol monitor, PM2.5 was measured for 30 min at each bus stop in two seasons. ‘Peak analysis' method was developed to assess short term PM2.5 exposure by OTS. The 30-min average PM2.5 exposure at each bus stop was associated with season and bus stop location but not smoking activity. The PM2.5 peak occurrence rate by the peak analysis method was significantly associated with season, bus stop location, observed smoking occurrence, and the number of buses servicing a route. The PM2.5 peak concentration was significantly associated with season, smoking occurrence, and the number of buses servicing a route. When a smoker was standing still at the bus stop, magnitude of peak concentrations were significantly higher than when the smoker walking-through the bus stop. People were exposed to high short-term PM2.5 peak levels at bus stops, and the magnitude of peak concentrations were highest when a smoker was located close to the monitor. The magnitude of peak concentration was a good indicator helped distinguish nearby OTS exposure. Further research using ‘peak analysis' is needed to measure smoking-related exposure to PM2.5 in other outdoor locations.

  13. 12 CFR 563b.115 - How will OTS review my business plan?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... FROM MUTUAL TO STOCK FORM Standard Conversions Prior to Conversion § 563b.115 How will OTS review my... with the Regional Office. OTS may request additional information, if necessary, to support...

  14. 12 CFR 563b.115 - How will OTS review my business plan?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... FROM MUTUAL TO STOCK FORM Standard Conversions Prior to Conversion § 563b.115 How will OTS review my... with the Regional Office. OTS may request additional information, if necessary, to support...

  15. 12 CFR 563b.115 - How will OTS review my business plan?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... FROM MUTUAL TO STOCK FORM Standard Conversions Prior to Conversion § 563b.115 How will OTS review my... with the Regional Office. OTS may request additional information, if necessary, to support...

  16. 12 CFR 563b.115 - How will OTS review my business plan?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... FROM MUTUAL TO STOCK FORM Standard Conversions Prior to Conversion § 563b.115 How will OTS review my... with the Regional Office. OTS may request additional information, if necessary, to support...

  17. Counterpart of MASTER OT J084140.94+722732.6

    NASA Astrophysics Data System (ADS)

    Nesci, Roberto

    2016-02-01

    Following the ATel #8725 by Balanutsa et al. about MASTER OT J084140.94+722732.6, I made aperture photometry on all the Palomar survey plates (12) available from the MAST archive to get an indication of the star color.

  18. 12 CFR 563.143 - Must I file with OTS?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Must I file with OTS? 563.143 Section 563.143... (including the proposed capital distribution) for the applicable calendar year exceeds your net income for that year to date plus your retained net income for the preceding two years Must file an...

  19. 12 CFR 500.10 - The OTS or The Office.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false The OTS or The Office. 500.10 Section 500.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY AGENCY ORGANIZATION AND... supervision of savings associations by regulatory staff to ensure the safety and soundness of the industry....

  20. 12 CFR 550.560 - When may the OTS revoke my fiduciary powers?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false When may the OTS revoke my fiduciary powers? 550.560 Section 550.560 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY... § 550.560 When may the OTS revoke my fiduciary powers? The OTS may revoke your fiduciary powers if...

  1. 12 CFR 516.270 - How long is the OTS review period?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How long is the OTS review period? 516.270 Section 516.270 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES OTS Review Standard Treatment § 516.270 How long is the OTS review period? (a)...

  2. 12 CFR 516.270 - How long is the OTS review period?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 6 2012-01-01 2012-01-01 false How long is the OTS review period? 516.270 Section 516.270 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES OTS Review Standard Treatment § 516.270 How long is the OTS review period? (a)...

  3. 12 CFR 516.270 - How long is the OTS review period?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true How long is the OTS review period? 516.270 Section 516.270 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES OTS Review Standard Treatment § 516.270 How long is the OTS review period? (a)...

  4. 12 CFR 563b.115 - How will OTS review my business plan?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How will OTS review my business plan? 563b.115... business plan? (a) OTS will review your business plan to determine that it demonstrates a safe and sound... will be determinative. OTS will review every case on its merits. (b) You must file your business...

  5. 12 CFR 585.120 - What factors will OTS consider in reviewing my application?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false What factors will OTS consider in reviewing my application? 585.120 Section 585.120 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE... OTS consider in reviewing my application? (a) OTS review. (1) In determining whether to approve...

  6. Teaching the Moving Child: OT Insights That Will Transform Your K-3 Classroom

    ERIC Educational Resources Information Center

    Berkey, Sybil M.

    2009-01-01

    Because sensorimotor and environmental factors have a profound effect on children's learning, every teacher should know how to weave strategies from occupational therapy (OT) into their everyday instruction. This is the guidebook K-3 teachers need to "think like an OT"--and form effective partnerships with OTs in their schools--so all students can…

  7. 12 CFR 550.120 - What action will the OTS take on my application?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false What action will the OTS take on my application... FIDUCIARY POWERS OF SAVINGS ASSOCIATIONS Obtaining Fiduciary Powers § 550.120 What action will the OTS take... OTS may impose conditions to ensure that the requirements of this part are met....

  8. 12 CFR 563b.200 - What actions may OTS take on my application?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false What actions may OTS take on my application....200 What actions may OTS take on my application? (a) OTS may approve your application for conversion only if: (1) Your conversion complies with this part; (2) You will meet your regulatory...

  9. Atomic force microscopy combined with optical tweezers (AFM/OT)

    NASA Astrophysics Data System (ADS)

    Pierini, F.; Zembrzycki, K.; Nakielski, P.; Pawłowska, S.; Kowalewski, T. A.

    2016-02-01

    The role of mechanical properties is essential to understand molecular, biological materials, and nanostructures dynamics and interaction processes. Atomic force microscopy (AFM) is the most commonly used method of direct force evaluation, but due to its technical limitations this single probe technique is unable to detect forces with femtonewton resolution. In this paper we present the development of a combined atomic force microscopy and optical tweezers (AFM/OT) instrument. The focused laser beam, on which optical tweezers are based, provides us with the ability to manipulate small dielectric objects and to use it as a high spatial and temporal resolution displacement and force sensor in the same AFM scanning zone. We demonstrate the possibility to develop a combined instrument with high potential in nanomechanics, molecules manipulation and biological studies. AFM/OT equipment is described and characterized by studying the ability to trap dielectric objects and quantifying the detectable and applicable forces. Finally, optical tweezers calibration methods and instrument applications are given.

  10. Optimization of OT-MACH Filter Generation for Target Recognition

    NASA Technical Reports Server (NTRS)

    Johnson, Oliver C.; Edens, Weston; Lu, Thomas T.; Chao, Tien-Hsin

    2009-01-01

    An automatic Optimum Trade-off Maximum Average Correlation Height (OT-MACH) filter generator for use in a gray-scale optical correlator (GOC) has been developed for improved target detection at JPL. While the OT-MACH filter has been shown to be an optimal filter for target detection, actually solving for the optimum is too computationally intensive for multiple targets. Instead, an adaptive step gradient descent method was tested to iteratively optimize the three OT-MACH parameters, alpha, beta, and gamma. The feedback for the gradient descent method was a composite of the performance measures, correlation peak height and peak to side lobe ratio. The automated method generated and tested multiple filters in order to approach the optimal filter quicker and more reliably than the current manual method. Initial usage and testing has shown preliminary success at finding an approximation of the optimal filter, in terms of alpha, beta, gamma values. This corresponded to a substantial improvement in detection performance where the true positive rate increased for the same average false positives per image.

  11. Fixed ground antenna radome (FGAR) type 1/3 OT&E integrations and OT&E operational

    NASA Astrophysics Data System (ADS)

    Baker, Leonard H.; Sedgwick, Harold G.

    1995-05-01

    This test report documents the Operational Test and Evaluation (OT&E) Integration and OT&E Operational testing performed on the Type I/Ill, Fixed Ground Antenna Radome (FGAR). The Type I/Ill FGAR is used with the Air Route Surveillance Radar (ARSR)) -1/2 and AN/FPS military radars. The testing was performed at the Federal Aviation Administration (FAA) Technical Center's Elwood En Route Beacon Test Facility (ERBTF) and the Northwest Mountain Region's Trinidad En Route Radar Facility (TAD), Colorado. The testing included: (1) characterization of the primary and secondary radar's electromagnetic performance; (2) human factors; (3) physical characteristics; and (4) physical performance. The electromagnetic performance testing showed no degradation of the primary or secondary radars; there were no human factor problems found; and only minor problems were identified during the physical characteristics and physical performance tests. The testing determined that the FGAR meets the Operational Suitability and Operational Effectiveness requirements of the FAA.

  12. Regulation of functional KCNQ1OT1 lncRNA by β-catenin

    PubMed Central

    Sunamura, Naohiro; Ohira, Takahito; Kataoka, Miki; Inaoka, Daigo; Tanabe, Hideyuki; Nakayama, Yuji; Oshimura, Mitsuo; Kugoh, Hiroyuki

    2016-01-01

    Long noncoding RNAs (lncRNAs) have been implicated in many biological processes through epigenetic mechanisms. We previously reported that KCNQ1OT1, an imprinted antisense lncRNA in the human KCNQ1 locus on chromosome 11p15.5, is involved in cis-limited silencing within an imprinted KCNQ1 cluster. Furthermore, aberration of KCNQ1OT1 transcription was observed with a high frequency in colorectal cancers. However, the molecular mechanism of the transcriptional regulation and the functional role of KCNQ1OT1 in colorectal cancer remain unclear. Here, we show that the KCNQ1OT1 transcriptional level was significantly increased in human colorectal cancer cells in which β-catenin was excessively accumulated in the nucleus. Additionally, overexpression of β-catenin resulted in an increase in KCNQ1OT1 lncRNA-coated territory. On the other hand, knockdown of β-catenin resulted in significant decrease of KCNQ1OT1 lncRNA-coated territory and an increase in the mRNA expression of the SLC22A18 and PHLDA2 genes that are regulated by KCNQ1OT1. We showed that β-catenin can promote KCNQ1OT1 transcription through direct binding to the KCNQ1OT1 promoter. Our evidence indicates that β-catenin signaling may contribute to development of colorectal cancer by functioning as a novel lncRNA regulatory factor via direct targeting of KCNQ1OT1. PMID:26868975

  13. 75 FR 24775 - Open Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-05

    ...The OTS Mutual Savings Associations Advisory Committee (MSAAC) will convene a telephonic meeting on Friday, May 21, 2010, beginning at 2 p.m. Eastern Time. The telephonic meeting will be open to the public. Members of the public interested in listening to the meeting and members of the public who require auxiliary aid should e-mail OTS at mutualcommittee@ots.treas.gov or call (202) 906-6429 to......

  14. The ALMA OT in early science: supporting multiple customers

    NASA Astrophysics Data System (ADS)

    Bridger, Alan; Williams, Stewart; McLay, Stewart; Yatagai, Hiroshi; Schilling, Marcus; Biggs, Andrew; Tobar, Rodrigo; Warmels, Rein H.

    2012-09-01

    The ALMA Observatory is currently operating 'Early Science' observing. The Cycle0 and Cycle1 Calls for Proposals are part of this Early Science, and in both the ALMA Observing Tool plays a crucial role. This paper describes how the ALMA OT tackles the problem of making millimeter/sub-millimeter interferometry accessible to the wider community, while allowing "experts" the power and flexibility they need. We will also describe our approach to the challenges of supporting multiple customers, and explore the lessons learnt from the Early Science experiences. Finally we look ahead to the challenges presented by future observing cycles.

  15. Transport of charged Aerosol OT inverse micelles in nonpolar liquids.

    PubMed

    Karvar, Masoumeh; Strubbe, Filip; Beunis, Filip; Kemp, Roger; Smith, Ashley; Goulding, Mark; Neyts, Kristiaan

    2011-09-01

    Surfactants such as Aerosol OT (AOT) are commonly used to stabilize and electrically charge nonpolar colloids in devices such as electronic ink displays. The electrical behavior of such devices is strongly influenced by the presence of charged inverse micelles, formed by excess surfactant that does not cover the particles. The presence of charged inverse micelles results in increased conductivity of the solution, affecting both the energy consumption of the device and its switching characteristics. In this work, we use transient current measurements to investigate the electrical properties of suspensions of the surfactant Aerosol OT in dodecane. No particles are added, to isolate the effect of excess surfactant. The measured currents upon application of a voltage step are found to be exponentially decaying, and can be described by an analytical model based on an equivalent electric circuit. This behavior is physically interpreted, first by the high generation rate of charged inverse micelles giving the suspension resistor like properties, and second by the buildup of layers of charged inverse micelles at both electrodes, acting as capacitors. The model explains the measurements over a large range of surfactant concentrations, applied voltages, and device thicknesses. PMID:21728309

  16. MASTER-IAC: QSO 2QZ J132407-0032 optical flare and OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Shurpakov, S.; Lipunov, V.; Gorbovskoy, E.; Rebolo, R.; Ricart, M. Serra; Israelian, G.; Lodieu, N.; Tiurina, N.; Kornilov, V.; Gress, O.; Kuznetsov, A.; Gorbunov, I.; Vlasenko, D.; Shumkov, V.; Vladimirov, V.

    2016-02-01

    MASTER-IAC auto-detection system( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 03h 32m 39.21s +19d 32m 15.0s on 2016-02-12.86192 UT. The OT unfiltered magnitude is 17.1 (limit 19.2m).

  17. 12 CFR 555.300 - Must I inform OTS before I use electronic means or facilities?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Must I inform OTS before I use electronic means... THE TREASURY ELECTRONIC OPERATIONS Requirements Applicable to All Savings Associations § 555.300 Must I inform OTS before I use electronic means or facilities? (a) General. A savings association...

  18. 12 CFR 516.10 - How does OTS compute time periods under this part?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false How does OTS compute time periods under this part? 516.10 Section 516.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES § 516.10 How does OTS compute time periods under this part? In...

  19. 12 CFR 516.10 - How does OTS compute time periods under this part?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How does OTS compute time periods under this part? 516.10 Section 516.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES § 516.10 How does OTS compute time periods under this part? In...

  20. 12 CFR 516.10 - How does OTS compute time periods under this part?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 6 2013-01-01 2012-01-01 true How does OTS compute time periods under this part? 516.10 Section 516.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES § 516.10 How does OTS compute time periods under this part? In...

  1. 12 CFR 516.10 - How does OTS compute time periods under this part?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true How does OTS compute time periods under this part? 516.10 Section 516.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES § 516.10 How does OTS compute time periods under this part? In...

  2. 12 CFR 516.10 - How does OTS compute time periods under this part?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 6 2012-01-01 2012-01-01 false How does OTS compute time periods under this part? 516.10 Section 516.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES § 516.10 How does OTS compute time periods under this part? In...

  3. 12 CFR 502.25 - How does OTS determine my complexity component?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How does OTS determine my complexity component... determine my complexity component? If you are a savings association and your portfolio exceeds any of the thresholds in paragraph (a) of this section, OTS will calculate your complexity component according...

  4. 75 FR 61572 - Open Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Office of Thrift Supervision Open Meeting of the OTS Mutual Savings Association Advisory Committee AGENCY: Department of the Treasury, Office of Thrift Supervision. ACTION: Notice of meeting. SUMMARY: The OTS...

  5. 12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 6 2012-01-01 2012-01-01 false When will OTS adjust, add, waive, or eliminate... TREASURY ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under unusual circumstances, the Director may deem it necessary or appropriate to adjust, add, waive,...

  6. 12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true When will OTS adjust, add, waive, or eliminate a... ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under unusual circumstances, the Director may deem it necessary or appropriate to adjust, add, waive, or eliminate a fee....

  7. 12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 6 2013-01-01 2012-01-01 true When will OTS adjust, add, waive, or eliminate a... ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under unusual circumstances, the Director may deem it necessary or appropriate to adjust, add, waive, or eliminate a fee....

  8. 12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false When will OTS adjust, add, waive, or eliminate... TREASURY ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under unusual circumstances, the Director may deem it necessary or appropriate to adjust, add, waive,...

  9. 12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false When will OTS adjust, add, waive, or eliminate... TREASURY ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under unusual circumstances, the Director may deem it necessary or appropriate to adjust, add, waive,...

  10. 12 CFR 563b.295 - Will OTS require me to re-solicit proxies?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Will OTS require me to re-solicit proxies? 563b.295 Section 563b.295 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY... to re-solicit proxies? If you amend your application for conversion, OTS may require you to...

  11. 12 CFR 563b.295 - Will OTS require me to re-solicit proxies?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false Will OTS require me to re-solicit proxies? 563b.295 Section 563b.295 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY... to re-solicit proxies? If you amend your application for conversion, OTS may require you to...

  12. 12 CFR 516.250 - Will OTS require me to publish a new public notice?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Will OTS require me to publish a new public... publish a new public notice? (a) If your application was subject to a publication requirement, OTS may require you to publish a new public notice of your application if: (1) You submitted a revision to...

  13. Radiation Susceptibility Assessment of Off the Shelf (OTS) Hardware

    NASA Technical Reports Server (NTRS)

    Culpepper, William X.; Nicholson, Leonard L. (Technical Monitor)

    2000-01-01

    The reduction in budgets, shortening of schedules and necessity of flying near state of the art technology have forced projects and designers to utilize not only modern, non-space rated EEE parts but also OTS boards, subassemblies and systems. New instrumentation, communications, portable computers and navigation systems for the International Space Station, Space Shuttle, and Crew Return Vehicle are examples of the realization of this paradigm change at the Johnson Space Center. Because of this change, there has been a shift in the radiation assessment methodology from individual part testing using low energy heavy ions to board and box level testing using high-energy particle beams. Highlights of several years of board and system level testing are presented along with lessons learned, present areas of concern, insights into test costs, and future challenges.

  14. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  15. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  16. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  17. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  18. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  19. 12 CFR 563b.240 - What must I submit to OTS after the members' meeting?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Vote by Members § 563b.240 What must I... the following information with OTS: (1) A certified copy of each adopted resolution on the...

  20. 12 CFR 563b.240 - What must I submit to OTS after the members' meeting?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Vote by Members § 563b.240 What must I... the following information with OTS: (1) A certified copy of each adopted resolution on the...

  1. 12 CFR 563b.240 - What must I submit to OTS after the members' meeting?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Vote by Members § 563b.240 What must I... the following information with OTS: (1) A certified copy of each adopted resolution on the...

  2. 12 CFR 563b.240 - What must I submit to OTS after the members' meeting?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Vote by Members § 563b.240 What must I... the following information with OTS: (1) A certified copy of each adopted resolution on the...

  3. 12 CFR 563b.240 - What must I submit to OTS after the members' meeting?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Vote by Members § 563b.240 What must I... the following information with OTS: (1) A certified copy of each adopted resolution on the...

  4. Vibrational energy relaxation of water in Aerosol OT reverse micelle

    NASA Astrophysics Data System (ADS)

    Pang, Yoonsoo; Deak, John; Dlott, Dana

    2005-03-01

    An IR-Raman technique with mid-IR pump and anti-Stokes Raman probe is used to investigate reverse micelle mixture of Aerosol OT, water, and carbon tetrachloride, where polar water phase and nonpolar oil phase is separated by a monolayer of surfactant molecules. Anti-Stokes Raman scattering is only dependent on the population of vibrationally excited states, thus time-dependent population changes of parent/daughter vibrations can be monitored with this technique. Vibrational energy from nanodroplet of water is transferred to the surfactant head group in 1.8 ps and then out to solvent in 10 ps. Vibrational energy directly pumped into the surfactant tail group results in a slower 20-40 ps energy transfer to solvent. This energy transfer cannot be explained by ordinary heat transfer, but the specific vibrational energy relaxation pathway such as sulfonate stretch of surfactant molecules should be used. We can change the water-to-solvent energy transfer rate by adopting different size of reverse micelles or changing pump frequency over the broad OH stretch mode of water due to hydrogen bond network. Water molecules confined in nanometer scale reverse micelles have very different properties from bulk water and we have found many differences between the vibrational dynamics of water in these reverse micelles and those of bulk water.

  5. OT2_ccodella_2: Where is chlorine in shocked regions?

    NASA Astrophysics Data System (ADS)

    Codella, C.

    2011-09-01

    As part of the GT Herschel Program CHESS we detected for the first time hydrogen chlorine in a protostellar shock, L1157-B1 (Codella et al. 2011). One of the most surprising results of this work was the lack of enhancement in the abundance of HCl with respect to dense interstellar clouds, implying that HCl is not enhanced by the passage of a shock. This means that either chlorine is not sputtered during the passage of the shock (unlikely as Si is sputtered) or that HCl is not the main reservoir of clorine in shocked regions (unlike in dense interstellar clouds). In this proposal we propose to observe HCl in a sample of shocked regions in order to determine whether this result is unique to L1157-B1. We stress that given the weakness of the HCl emission in shocks and the strong atmospheric water absorption at the requested frequency (626 GHz), the present experiment cannot be reasonably performed from ground, making of Herschel OT2 the last chance to reach the present goals.

  6. Coexisting aggregates in mixed aerosol OT and cholesterol microemulsions.

    PubMed

    Sedgwick, Myles A; Trujillo, Alejandro M; Hendricks, Noah; Levinger, Nancy E; Crans, Debbie C

    2011-02-01

    Dynamic light scattering and NMR spectroscopic experimental evidence suggest the coexistence of two compositionally different self-assembled particles in solution. The self-assembled particles form in solutions containing water, Aerosol OT (AOT, sodium bis(2-ethylhexyl) sulfosuccinate) surfactant, and cholesterol in cyclohexane. In a similar series of studies carried out in 1-octanol only one aggregate type, that is, reverse micelles, is observed. Dynamic light scattering measurements reveal the presence of two different types of aggregates in the microemulsions formed in cyclohexane, demonstrating the coexistence of two compositionally distinct structures with very similar Gibbs energies. One particle type consists of standard AOT reverse micelles while the second type of particle consists of submicellar aggregates including cholesterol as well as small amounts of AOT and water. In microemulsions employing 1-octanol as the continuous medium, AOT reverse micelles form in a dispersed solution of cholesterol in 1-octanol. Although the size distribution of self-assembled particles is well-known for many different systems, evidence for simultaneous formation of two distinctly sized particles in solution that are chemically different is unprecedented. The ability to form microemulsion solutions that contain coexisting particles may have important applications in drug formulation and administration, particularly as applied to drug delivery using cholesterol as a targeting agent. PMID:21188993

  7. Effects of Hypergravity Exposure on Plasma Oxytocin (OT) Concentrations in Pregnant and Lactating Rat Dams

    NASA Technical Reports Server (NTRS)

    Baer, Lisa A.; Wade, Charles E.; Plaut, Karen; Ronca, April E.; Dalton, Bonnie (Technical Monitor)

    2002-01-01

    From pregnancy to weaning there is a progressive elevation of plasma oxytocin (OT) levels associated with nursing activity, irrespective of litter size. In the present study, we analyzed the effects of continuous 1.5G, 1.75G and 2.0G hypergravity exposure on OT plasma concentration in prepartum (Gestation Day 20) (G20) and lactating (Postnatal day) (P10) rat dams. For this study, litter size was controlled with a yoking procedure established in our lab where individual control litters were yoked-matched to individual hypergravity litters. We reviewed all data at hypergravity irrespective of gravitational level and compared the values with the controls in both G20 (HG, n=15;SC, n=9) and P10 (HG, n=21;SC, n=16). Results showed that over time, we did observe the expected OT increase in both groups. In G20 dams, measurement of OT concentrations showed no significance. However, at P10, measurements of OT concentrations suggest a reduction of about 20% compared to established controls in our laboratory, 0.9+/-0.09 ng/ml for the controls and 0.7+/-0.06 ng/ml for centrifuged animals (p<0.02). These data suggest that exposure to centrifugation may reduce OT levels during lactation. When these plasma samples were obtained, the dams were removed from the litters, and values were not adjusted for the size of the litters. The reduction in OT with centrifugation may reflect a decrease in nursing activity or a decreased responsiveness of the mammary hypothalamic axis. In addition, we have analyzed data on plasma prolactin concentrations and mammary gland development, which may give additional insight to the results of our OT measurements.

  8. Oxytocin (OT) and arginine-vasopressin (AVP) act on OT receptors and not AVP V1a receptors to enhance social recognition in adult Syrian hamsters (Mesocricetus auratus).

    PubMed

    Song, Zhimin; Larkin, Tony E; Malley, Maureen O'; Albers, H Elliott

    2016-05-01

    Social recognition is a fundamental requirement for all forms of social relationships. A majority of studies investigating the neural mechanisms underlying social recognition in rodents have investigated relatively neutral social stimuli such as juveniles or ovariectomized females over short time intervals (e.g., 2h). The present study developed a new testing model to study social recognition among adult males using a potent social stimulus. Flank gland odors are used extensively in social communication in Syrian hamsters and convey important information such as dominance status. We found that the recognition of flank gland odors after a 3min exposure lasted for at least 24h, substantially longer than the recognition of other social cues in rats and mice. Intracerebroventricular injections of OT and AVP prolonged the recognition of flank gland odor for up to 48h. Selective OTR but not V1aR agonists, mimicked these enhancing effects of OT and AVP. Similarly, selective OTR but not V1aR antagonists blocked recognition of the odors after 20min. In contrast, the recognition of non-social stimuli was not blocked by either the OTR or the V1aR antagonists. Our findings suggest both OT and AVP enhance social recognition via acting on OTRs and not V1aRs and that the recognition enhancing effects of OT and AVP are limited to social stimuli. PMID:26975586

  9. OT2_smalhotr_3: Herschel Extreme Lensing Line Observations (HELLO)

    NASA Astrophysics Data System (ADS)

    Malhotra, S.

    2011-09-01

    We request 59.8 hours of Herschel time to observe 20 normal star-forming galaxies in the [CII] 158 micron and [OI] 63 micron lines. These galaxies lie at high redshift (1OT1, 49 high redshift IR luminous galaxies were approved for spectroscopy, but only two so-called normal galaxies were included. This is an imbalance that should be corrected, to balance Herschel's legacy.

  10. OmniTread OT-4 serpentine robot: new features and experiments

    NASA Astrophysics Data System (ADS)

    Borenstein, Johann; Hansen, Malik

    2007-04-01

    Serpentine robots are slender, multi-segmented vehicles designed to provide greater mobility than conventional wheeled or tracked robots. Serpentine robots are thus ideally suited for urban search and rescue, military intelligence gathering, and for surveillance and inspection tasks in hazardous and hard-to-reach environments. One such serpentine robot, developed at the University of Michigan, is the "OmniTread OT-4." The OT-4 comprises seven segments, which are linked to each other by 2-degree-of-freedom joints. The OT-4 can climb over obstacles that are much higher than the robot itself, propel itself inside pipes of different diameters, and traverse even the most difficult terrain, such as rocks or the rubble of a collapsed structure. The foremost and unique design characteristic of the OT-4 is the use of pneumatic bellows to actuate the joints. These bellows allow simultaneous control of position and stiffness for each joint. Controllable stiffness is of crucial importance in serpentine robots, which require stiff joints to cross gaps and compliant joints to conform to rough terrain for effective propulsion. Another unique feature of the OmniTread design is the maximal coverage of all four sides with driven tracks. This design makes the robot indifferent to roll-overs, which are happen frequently when the slender bodies of serpentine robots travel over rugged terrain. This paper describes the OmniTread concept as well as its latest technical features, and an extensive Experiment Results Section documents the abilities of the OT-4.

  11. Expression and Developmental Regulation of Oxytocin (OT) and Oxytocin Receptors (OTR) in the Enteric Nervous System (ENS) and Intestinal Epithelium

    PubMed Central

    Welch, Martha G.; Tamir, Hadassah; Gross, Kara J.; Chen, Jason; Anwar, Muhammad; Gershon, Michael D.

    2011-01-01

    Although oxytocin (OT) and oxytocin receptor (OTR) are known for roles in parturition and milk let-down, they are not hypothalamus-restricted. OT is important in nurturing and opposition to stress. Transcripts encoding OT and OTR have been reported in adult human gut, and OT affects intestinal motility. We tested the hypotheses that OT is endogenous to the enteric nervous system (ENS) and that OTR signaling may participate in enteric neurophysiology. Reverse transcriptase polymerase chain reaction confirmed OT and OTR transcripts in adult mouse and rat gut and in precursors of enteric neurons immunoselected from fetal rats. Enteric OT and OTR expression continued through adulthood but was developmentally regulated, peaking at postnatal day 7. Coincidence of the immunoreactivities of OTR and the neural marker Hu was 100% in the P3 and 71% in the adult myenteric plexus, when submucosal neurons were also OTR-immunoreactive. Co-localization with NeuN established that intrinsic primary afferent neurons are OTR-expressing. Because OTR transcripts and protein were detected in the nodose ganglia, OT signaling might also affect extrinsic primary afferent neurons. Although OT immunoreactivity was found only in ~1% of myenteric neurons, extensive OT-immunoreactive varicosities surrounded many others. Villus enterocytes were OTR-immunoreactive through postnatal day 17; however, by postnatal day 19, immunoreactivity waned to become restricted to crypts and concentrated at crypt-villus junctions. Immunoelectron microscopy revealed plasmalemmal OTR at enterocyte adherens junctions. We suggest that OT and OTR signaling might be important in ENS development and function and might play roles in visceral sensory perception and neural modulation of epithelial biology. PMID:19003903

  12. MASTER OT J122114.34+472950.7 is a Type Ia Supernova

    NASA Astrophysics Data System (ADS)

    Silverman, J. M.; Tucker, B. E.; Vinko, J.; Quimby, R.; Wheeler, J. C.; Chatzopoulos, E.

    2013-04-01

    We write that a spectrogram, obtained on Mar. 31.18 UT with the 9.2-m Hobby-Eberly Telescope (+ Marcario Low-Resolution Spectrograph) by J. Caldwell, shows that MASTER OT J122114.34+472950.7 is a type-Ia supernova. Correlation with a library of supernova spectra using the "SuperNova IDentification" code (SNID; Blondin and Tonry 2007, Ap.J. 666, 1024) indicates that MASTER OT J122114.34+472950.7 is SN 1999aa-like type-Ia supernova (Strolger, et al., 2002, A.J., 124, 2905; Garavini, et al., 2004, A.J., 128, 387) at maximum brightness.

  13. A global outer-rise/outer-trench-slope (OR/OTS) earthquake study

    NASA Astrophysics Data System (ADS)

    Wartman, J. M.; Kita, S.; Kirby, S. H.; Choy, G. L.

    2009-12-01

    Using improved seismic, bathymetric, satellite gravity and other geophysical data, we investigated the seismicity patterns and focal mechanisms of earthquakes in oceanic lithosphere off the trenches of the world that are large enough to be well recorded at teleseismic distances. A number of prominent trends are apparent, some of which have been previously recognized based on more limited data [1], and some of which are largely new [2-5]: (1) The largest events and the highest seismicity rates tend to occur where Mesozoic incoming plates are subducting at high rates (e.g., those in the western Pacific and the Banda segment of Indonesia). The largest events are predominantly shallow normal faulting (SNF) earthquakes. Less common are reverse-faulting (RF) events that tend to be deeper and to be present along with SNF events where nearby seamounts, seamount chains and other volcanic features are subducting [Seno and Yamanaka, 1996]. Blooms of SNF OR/OTS events usually occur just after and seaward of great interplate thrust (IPT) earthquakes but are far less common after smaller IPT events. (2) Plates subducting at slow rates (<20 mm/a) often show sparse OR/OTS seismicity. It is unclear if such low activity is a long-term feature of these systems or is a consequence of the long return times of great IPT earthquakes (e.g., the sparse OR/OTS seismicity before the 26 December 2004 M9.2 Sumatra earthquake and many subsequent OR/OTS events). (3) OR/OTS shocks are generally sparse or absent where incoming plates are very young (<20 Ma) (e.g., Cascadia, southern Mexico, Nankai, and South Shetlands). (4) Subducting plates of intermediate age (20 to about 65 Ma) display a diversity of focal mechanisms and seismicity patterns. In the Philippines, NE Indonesia, and Melanesia, bands of reverse faulting events occur at or near the trench and SNF earthquakes are restricted to OR/OTS sites further from the trench. (5) Clustering of OR/OTS events of all types commonly occurs where

  14. Long non-coding RNA SOX2OT: expression signature, splicing patterns, and emerging roles in pluripotency and tumorigenesis

    PubMed Central

    Shahryari, Alireza; Jazi, Marie Saghaeian; Samaei, Nader M.; Mowla, Seyed J.

    2015-01-01

    SOX2 overlapping transcript (SOX2OT) is a long non-coding RNA which harbors one of the major regulators of pluripotency, SOX2 gene, in its intronic region. SOX2OT gene is mapped to human chromosome 3q26.3 (Chr3q26.3) locus and is extended in a high conserved region of over 700 kb. Little is known about the exact role of SOX2OT; however, recent studies have demonstrated a positive role for it in transcription regulation of SOX2 gene. Similar to SOX2, SOX2OT is highly expressed in embryonic stem cells and down-regulated upon the induction of differentiation. SOX2OT is dynamically regulated during the embryogenesis of vertebrates, and delimited to the brain in adult mice and human. Recently, the disregulation of SOX2OT expression and its concomitant expression with SOX2 have become highlighted in some somatic cancers including esophageal squamous cell carcinoma, lung squamous cell carcinoma, and breast cancer. Interestingly, SOX2OT is differentially spliced into multiple mRNA-like transcripts in stem and cancer cells. In this review, we are describing the structural and functional features of SOX2OT, with an emphasis on its expression signature, its splicing patterns and its critical function in the regulation of SOX2 expression during development and tumorigenesis. PMID:26136768

  15. 12 CFR 563b.515 - What information must I provide to OTS before I repurchase my shares?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 6 2013-01-01 2012-01-01 true What information must I provide to OTS before I... THE TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Post-Conversion § 563b.515 What information must I provide to OTS before I repurchase my shares? (a) To repurchase stock in...

  16. 12 CFR 563b.515 - What information must I provide to OTS before I repurchase my shares?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 6 2012-01-01 2012-01-01 false What information must I provide to OTS before I... THE TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Post-Conversion § 563b.515 What information must I provide to OTS before I repurchase my shares? (a) To repurchase stock in...

  17. 12 CFR 563b.515 - What information must I provide to OTS before I repurchase my shares?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false What information must I provide to OTS before I... THE TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Post-Conversion § 563b.515 What information must I provide to OTS before I repurchase my shares? (a) To repurchase stock in...

  18. 12 CFR 563b.515 - What information must I provide to OTS before I repurchase my shares?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true What information must I provide to OTS before I... THE TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Post-Conversion § 563b.515 What information must I provide to OTS before I repurchase my shares? (a) To repurchase stock in...

  19. 12 CFR 563b.515 - What information must I provide to OTS before I repurchase my shares?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false What information must I provide to OTS before I... THE TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Post-Conversion § 563b.515 What information must I provide to OTS before I repurchase my shares? (a) To repurchase stock in...

  20. 12 CFR 516.220 - If OTS requests additional information to complete my application, how will it process my...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... complete my application, how will it process my application? 516.220 Section 516.220 Banks and Banking... Standard Treatment § 516.220 If OTS requests additional information to complete my application, how will it... your response. OTS will notify you that it has extended the period before the end of the initial...

  1. 12 CFR 516.220 - If OTS requests additional information to complete my application, how will it process my...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... complete my application, how will it process my application? 516.220 Section 516.220 Banks and Banking... Standard Treatment § 516.220 If OTS requests additional information to complete my application, how will it... your response. OTS will notify you that it has extended the period before the end of the initial...

  2. Optimal Diphthongs: An OT Analysis of the Acquisition of Spanish Diphthongs

    ERIC Educational Resources Information Center

    Krause, Alice

    2013-01-01

    This dissertation investigates the acquisition of Spanish diphthongs by adult native speakers of English. The following research questions will be addressed: 1) How do adult native speakers of English pronounce sequences of two vowels in their L2 Spanish at different levels of acquisition? 2) Can OT learnability models, specifically the GLA,…

  3. MASTER OT J095503.19-355149.4 detection during FERMI Trigg 478121069 inspection

    NASA Astrophysics Data System (ADS)

    Lipunov, V.; Tiurina, N.; Gorbovskoy, E.; Buckley, D.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Gress, O.; Vlasenko, D.; Vladimirov, V.; Shumkov, V.; Shurpakov, S.; Kuvshinov, D.; Potter, S.; Kotze, M.

    2016-02-01

    MASTER-SAAO auto-detection system( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 09h 55m 03.19s -35d 51m 49.4s on 2016-02-25.89505 UT during Fermi trigger 478121069 inspection (Buckley et al., GCN #19092).

  4. OGLE-IV Pre-discovery Observations of MASTER OT J051032.58-692130.4

    NASA Astrophysics Data System (ADS)

    Mroz, P.; Udalski, A.; Wyrzykowski, L.; Pawlak, M.

    2016-05-01

    Gorbovskoy et al. (ATel #9039) reported the discovery of a bright optical transient MASTER OT J051032.58-692130.4 toward the Large Magellanic Cloud. The object is located in the field LMC509 that is regularly monitored by the OGLE-IV Survey.

  5. Swift observation of the LMC nova candidate MASTER OT J051032.58-692130.4

    NASA Astrophysics Data System (ADS)

    Sokolovsky, Kirill

    2016-05-01

    The optical transient MASTER & nbsp;OT & nbsp;J051032.58-692130.4 was discovered by MASTER-SAAO on 2016-05-10.72797 (ATel #9039). Pre-discovery images by OGLE-IV indicate that the outburst occurred some time between 2016-04-21.00403 and 28.97626 (ATel #9040).

  6. MASTER: young PSN(Ia) in bright PGC054958 and OTs

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Shurpakov, S.; Lipunov, V.; Lopez, R. Rebolo; Buckley, D.; Ricart, Serra; Israelyan, G.; Potter, S.; Kniazev, A.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Vlasenko, D.; Gorbunov, I.; Kuvshinov, D.; Vladimirov, V.; Popova, E.

    2016-04-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 15h 23m 33.04s +09d 21m 25.6s on 2016-04-04.09646 UT. PSN unfiltered magnitude is 17.7m (limit 19.5m).

  7. Pre-discovery observations of MASTER OT J004514.13+420007.2

    NASA Astrophysics Data System (ADS)

    Maehara, Hiroyuki

    2016-01-01

    I report pre-discovery observations of the possible nova in M31 MASTER OT J004514.13+420007.2 (ATel # 8603). The observations were performed with the 1.05-m Schmidt telescope and KWFC (Sako et al. 2012, SPIE, 8446, 84466L) at Kiso Observatory.

  8. Spectroscopic Classification of MASTER OT J110707.62-052244.0 as a Type Ia Supernova

    NASA Astrophysics Data System (ADS)

    Zheng, W.; Kim, M.; Shivvers, I.; Yuk, H.; Filippenko, A. V.

    2015-11-01

    We report that inspection of a CCD spectrum (range 350-1050 nm) of MASTER OT J110707.62-052244.0 (ATel #8236), obtained on Nov. 11.57 UT with the Shane 3-m reflector (+ Kast spectrograph) at Lick Observatory, shows that the object is a normal Type Ia supernova roughly 1 week past maximum brightness.

  9. MELATONIN ENHANCES JUNCTIONAL TRANSFER IN NORMAL C3H/1OT1/2 CELLS

    EPA Science Inventory

    There is strong evidence that pineal melatonin is involved in controlling neoplastic processes. e have reported that physiological, but not pharmacological or subphysiological, concentrations of melatonin enhance intercellular communication in normal C3H/1OT1/2 fibroblasts. ap ju...

  10. 75 FR 76524 - Closed Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-08

    ... Office of Thrift Supervision Closed Meeting of the OTS Mutual Savings Association Advisory Committee AGENCY: Office of Thrift Supervision, Department of the Treasury. ACTION: Notice of Closed Meeting... held at the Office of Thrift Supervision, 1700 G Street, NW., Washington, DC 20552. The public...

  11. 75 FR 38188 - Closed Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-01

    ... Office of Thrift Supervision Closed Meeting of the OTS Mutual Savings Association Advisory Committee AGENCY: Department of the Treasury, Office of Thrift Supervision. ACTION: Notice of Closed Meeting... meeting will be held at the Office of Thrift Supervision, 1700 G Street, NW., Washington, DC 20552....

  12. 76 FR 7630 - Open Meeting of the OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Office of Thrift Supervision Open Meeting of the OTS Minority Depository Institutions Advisory Committee AGENCY: Department of the Treasury, Office of Thrift Supervision. ACTION: Notice of meeting. SUMMARY:...

  13. 75 FR 13344 - Open Meeting of the OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Office of Thrift Supervision Open Meeting of the OTS Minority Depository Institutions Advisory Committee AGENCY: Department of the Treasury, Office of Thrift Supervision. ACTION: Notice of meeting. SUMMARY:...

  14. 75 FR 63895 - Open Meeting of the OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-18

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Office of Thrift Supervision Open Meeting of the OTS Minority Depository Institutions Advisory Committee AGENCY: Department of the Treasury, Office of Thrift Supervision. ACTION: Notice of meeting. SUMMARY:...

  15. 12 CFR 555.300 - Must I inform OTS before I use electronic means or facilities?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... transactional web site. A transactional web site is an Internet site that enables users to conduct financial... 12 Banks and Banking 6 2014-01-01 2012-01-01 true Must I inform OTS before I use electronic means or facilities? 555.300 Section 555.300 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT...

  16. 12 CFR 563b.300 - What must happen before OTS declares my offering circular effective?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... offering circular effective? 563b.300 Section 563b.300 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Offering Circular § 563b.300 What must happen before OTS declares my offering circular effective? (a) You must prepare...

  17. 12 CFR 563b.300 - What must happen before OTS declares my offering circular effective?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... offering circular effective? 563b.300 Section 563b.300 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Offering Circular § 563b.300 What must happen before OTS declares my offering circular effective? (a) You must prepare...

  18. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... mooring-oil transfer system (SPM-OTS) displaced with water? 150.445 Section 150.445 Navigation and...-OTS) displaced with water? (a) The oil in an SPM-OTS must be displaced with water, and the valve at... section is waived if port officials can demonstrate to the Officer in Charge of Marine Inspection that...

  19. 12 CFR 502.27 - How does OTS determine the risk/complexity component for a savings and loan holding company?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... loan holding company? (a) OTS computes the risk/complexity component for responsible savings and loan... entities. (c) OTS uses the following chart to compute the risk/complexity component under this section. OTS... Billion (d) To compute your risk/complexity component, find the row in the appropriate schedule...

  20. Distribution of polyphenols and a surfactant component in skin during Aerosol OT microemulsion-enhanced intradermal delivery.

    PubMed

    Yutani, Reiko; Morita, Shin-ya; Teraoka, Reiko; Kitagawa, Shuji

    2012-01-01

    As for most other polyphenols, intradermal delivery of curcumin and resveratrol is limited; however, it was significantly improved by a microemulsion using Aerosol OT (Aerosol OT microemulsion) and Tween 80 (Tween 80 microemulsion) as surfactants. Aerosol OT microemulsion was more effective and the incorporation ratio of these polyphenols into skin by Aerosol OT microemulsion was five-fold or ten-fold that by Tween 80 microemulsion. To clarify the mechanism of the enhancement we examined the distribution of these polyphenols and the surfactant component, Aerosol OT, using excised guinea pig skin and Yucatan micropig (YMP) skin. During permeation, polyphenols distributed deep in the skin. In particular, a small molecule, resveratrol, was mainly present in the dermis in YMP skin. Aerosol OT also distributed deep in the skin. These findings suggest the possible involvement of the interaction of surfactant molecules with skin components in the enhanced delivery process of polyphenols. The distribution ratio between the dermis and epidermis of the polyphenols, including quercetin, in the presence of Aerosol OT microemulsion decreased with the increase of molecular weight in YMP skin, suggesting the possibility that distribution to the dermis is regulated by the molecular size. PMID:22863702

  1. Computer software design description for the Treated Effluent Disposal Facility (TEDF), Project L-045H, Operator Training Station (OTS)

    SciTech Connect

    Carter, R.L. Jr.

    1994-11-07

    The Treated Effluent Disposal Facility (TEDF) Operator Training Station (OTS) is a computer-based training tool designed to aid plant operations and engineering staff in familiarizing themselves with the TEDF Central Control System (CCS).

  2. 12 CFR 502.29 - How does OTS determine the condition component for a savings and loan holding company?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... holding company structure) is a composite rating of 4 or 5, OTS will assess a charge under the condition... amount, the risk/complexity component, and any organizational form component. (b) For the purposes...

  3. 12 CFR 502.29 - How does OTS determine the condition component for a savings and loan holding company?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... holding company structure) is a composite rating of 4 or 5, OTS will assess a charge under the condition... amount, the risk/complexity component, and any organizational form component. (b) For the purposes...

  4. Asiago observation of the OT of GRB150413A detected by MASTER

    NASA Astrophysics Data System (ADS)

    Tomasella, L.; Benetti, S.; Cappellaro, E.; Elias-Rosa, N.; Ochner, P.; Pastorello, A.; Tartaglia, L.; Terreran, G.; Turatto, M.

    2015-04-01

    In the framework of the Asiago Transient Classification Program (Tomasella et al. 2014, AN, 335, 841), we have spectroscopically observed the OT of GRB150413A detected by MASTER (Atel #7381) with the Asiago 1.82 m Copernico Telescope (+AFOSC; range 340-820 nm; resolution 1.4 nm), on 20150413.87 UT, that is about 7.0h after detection.

  5. Asiago spectroscopic classification of MASTER OT J211223.35+144645.1

    NASA Astrophysics Data System (ADS)

    Ascenzi, S.; Yang, S.; Tomasella, L.; Benetti, S.; Cappellaro, E.; Pastorello, A.; Elias-Rosa, N.; Ochner, P.; Tartaglia, L.; Terreran, G.; Turatto, M.

    2016-06-01

    The Asiago Transient Classification Program (Tomasella et al. 2014, AN, 335, 841) reports the spectroscopic classification of MASTER OT J211223.35+144645.1 (Atel #9174), discovered by MASTER-IAC auto-detection system (Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171) The observation was performed with the Asiago 1.82 m Copernico Telescope (+AFOSC; range 340-820 nm; resolution 1.4 nm).

  6. 78 FR 76768 - Removal of Transferred OTS Regulations Regarding Disclosure and Reporting of CRA-Related...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ... on July 6, 2011.\\2\\ \\2\\ 76 FR 39247 (July 6, 2011). Although section 312(b)(2)(B)(i)(II) of the Dodd... rescinding them, as appropriate. \\3\\ 76 FR 47652 (Aug. 5, 2011). One of the OTS rules transferred to the FDIC...)(1). \\8\\ 66 FR 2052 (Jan. 10, 2001). \\9\\ Id. Section 48 of the FDI Act, created by section 711 of...

  7. MASTER OT J095503.19-355149.4 - a probable Dwarf Nova

    NASA Astrophysics Data System (ADS)

    Buckley, D. A. H.; Kniazev, A.; Potter, S. B.; Lipunov, V.; Tiurina, N.; Gorbovskoy, E.

    2016-02-01

    Followup optical imaging and spectroscopic observations with SALT of the optical transient MASTER OT J095503.19-355149.4, tentatively identified (ATel #8741) as the optical counterpart of the Fermi GBM trigger 478121068 (GCN #19092), were conducted on 2016-02-26.78 UT. From un-calibrated g', r' and i' exposures (30 sec each) with the SALTICAM CCD camera, the transient appeared to be approximately 1 mag brighter (V ~ 17) than the MASTER-SAAO observations of the previous night (reported in ATel #8741) and was also seen to be a relatively red object compared to other stars in the field.

  8. Complete Genome Sequence of the Larvicidal Bacterium Lysinibacillus sphaericus Strain OT4b.25

    PubMed Central

    Rey, Andrés; Silva-Quintero, Laura

    2016-01-01

    Lysinibacillus sphaericus OT4b.25 is a native Colombian strain isolated from coleopteran larvae in an oak forest near Bogotá D.C.; this strain has shown high levels of pathogenic activity against Culex quinquefasciatus larvae in laboratory assays compared to that of other members of the same species. Using Pacific Biosciences sequencing technology, we propose a chromosomal contig of 4,665,775 bp that, according to comparative analysis, is highly similar to that of reference strain L. sphaericus C3-41. PMID:27151786

  9. Spectroscopic classification of PSN J13144705+5405055 (= MASTER OT J131447.05+540505.5)

    NASA Astrophysics Data System (ADS)

    Vanni, S.; Pastorello, A.; Benetti, S.; Cappellaro, E.; Ochner, P.; Tomasella, L.; Turatto, M.

    2012-11-01

    We report that optical spectroscopy (range 340-820 nm; resolution 1.3 nm) obtained on Nov. 8.18 UT with the Asiago 1.82-m Copernico Telescope (+ AFOSC), shows that PSN J13144705+5405055 (= MASTER OT J131447.05+540505.5) is a type-Ia SN at a redshift z~0.033 (Sloan Digital Sky Survey Data Release 3). The best match was found with type-Ia SN 2002bo (Benetti et al. 2004, MNRAS, 348, 261) about 3-4 weeks after the B band maximum light.

  10. Genome sequence and description of the heavy metal tolerant bacterium Lysinibacillus sphaericus strain OT4b.31

    PubMed Central

    Peña-Montenegro, Tito David; Dussán, Jenny

    2013-01-01

    Lysinibacillus sphaericus strain OT4b.31 is a native Colombian strain having no larvicidal activity against Culex quinquefasciatus and is widely applied in the bioremediation of heavy-metal polluted environments. Strain OT4b.31 was placed between DNA homology groups III and IV. By gap-filling and alignment steps, we propose a 4,096,672 bp chromosomal scaffold. The whole genome (consisting of 4,856,302 bp long, 94 contigs and 4,846 predicted protein-coding sequences) revealed differences in comparison to the L. sphaericus C3-41 genome, such as syntenial relationships, prophages and putative mosquitocidal toxins. Sphaericolysin B354, the coleopteran toxin Sip1A and heavy metal resistance clusters from nik, ars, czc, cop, chr, czr and cad operons were identified. Lysinibacillus sphaericus OT4b.31 has applications not only in bioremediation efforts, but also in the biological control of agricultural pests. PMID:24501644

  11. Preparation and physicochemical characterization of dioctyl sodium sulfosuccinate (aerosol OT) microemulsion for oral drug delivery.

    PubMed

    El-Laithy, Hanan M

    2003-01-01

    The performance of dioctyl sodium sulfosuccinate (aerosol OT) in the development of a pharmaceutically acceptable, stable, self-emulsifying water continuous microemulsion with high dilution efficiency was assessed. A pseudoternary microemulsion system was constructed using aerosol OT/medium-chain triglycerides with oleic acid/glycerol monooleate and water. The model microemulsion was characterized with regard to its electroconductive behavior, eosin sodium absorption, interfacial tension, and droplet size measurements after dilution with water. The percolation transition law, which makes it possible to determine the percolation threshold and to identify bicontinuous structures, was applied to the system. The interfacial tension changes associated with the microemulsion formation revealed ultralow values up to 30% oil at a surfactant/cosurfactant ratio of 3:1. Moreover, the investigated particle size and polydispersity using photon correlation spectroscopy after dilution with excess of the continuous phase proved the efficiency of the microemulsion system as a drug carrier that ensures an infinitely dilutable, homogeneous, and thermodynamically stable system. PMID:12916920

  12. Solar-energy-system performance evaluation: Honeywell OTS 44, Ocmulgee, Georgia

    NASA Technical Reports Server (NTRS)

    Mathur, A. K.; Pederson, S.

    1982-01-01

    The operation and technical performance of the solar operational test site (OTS 44) are described, based on data collected between April, 1981 and August, 1981. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 44 is a hydronic heating and cooling system consisting of 5040 square feet of liquid cooled flat plate collectors; a 4000 gallon thermal storage tank; one 25 ton capacity organic Rankine cycle engine assisted water chillers; a forced draft cooling tower; and associated piping, pumps, valves, controls and heat rejection equipment. The solar system has eight basic modes of operation and several combination modes for providing space conditioning and hot water to the building. Data monitored during the 4 months of the operational test period found that the solar system collected 285 MMBtu of thermal energy of the total incident solar energy of 1040 MMBtu and provided 210 MMBtu for cooling and 10 MMBtu for heating and hot water. The net electrical energy saving due to the solar system was approximately 2600 kWh(e), and fossil energy saving was about 20 million Btu (MMBtu).

  13. SpOT the Correct Tissue Every Time in Multi-Tissue Blocks

    PubMed Central

    Coffey, Anna H.; Berry, Deborah L.; Johnson, Michael D.

    2015-01-01

    Multi-tissue paraffin blocks provide high throughput analysis with increased efficiency, experimental uniformity, and reduced time and cost. Tissue microarrays make up the majority of multi-tissue paraffin blocks, but increasingly, researchers are using non-arrayed blocks containing larger tissues from multiple individuals which can provide many of the advantages of tissue microarrays without substantial investment in planning and equipment. A critical component of any multi-tissue analysis is the orientation method used to identify each individual tissue. Although methods exist to maintain proper orientation and identification of tissues in multi-tissue blocks, most are not well-suited to non-arrayed blocks, may consume valuable space within an array and/or are difficult to produce in the standard histology laboratory. The Specimen Orientation Tag (SpOT) is a simple, low cost orientation tool that is clearly visible in paraffin blocks and all tissue sections for reliable specimen identification in arrayed and non-arrayed layouts. The SpOT provides advantages over existing orientation methods for non-arrayed blocks as it does not require any direct modification to the tissue and allows for flexibility in the arrangement of tissue pieces. PMID:26067587

  14. DeSSpOt: an instrument for stellar spin orientation determination

    NASA Astrophysics Data System (ADS)

    Lesage, Anna-Lea; Schneide, Magnus; Wiedemann, Günter

    2012-09-01

    We designed and constructed a special instrument to enable the determination of the stellar's spin orientation. The Differential image rotator for Stellar Spin Orientation, DeSSpOt, allows the simultaneous observations of two anti-parallel orientations of the star on the spectrum. On a high resolution échelle spectrum, the stellar rotation causes a slight line tilt visible in the spatial direction which is comparable to a rotation curve. We developed a new method, which exploits the variations in these tilts, to estimate the absolute position angle of the rotation axis. The line tilt is retrieved by a spectroastrometric extraction of the spectrum. In order to validate the method, we observed spectroscopic binaries with known orbital parameters. The determination of the orbital position angle is equivalent to the determination of the stellar position angle, but is easier to to detect. DeSSpOt was successfully implemented on the high resolution Coudé spectrograph of the Thüringer Landessternwarte Tautenburg. The observations of Capella led to the determination of the orbital position angle. Our value of 37.2° is in agreement with the values previously found in the literature. As such we verified that both method and instrument are valid.

  15. Pyrrole-imidazole polyamide-mediated silencing of KCNQ1OT1 expression induces cell death in Wilms' tumor cells.

    PubMed

    Yoshizawa, Shinsuke; Fujiwara, Kyoko; Sugito, Kiminobu; Uekusa, Shota; Kawashima, Hiroyuki; Hoshi, Reina; Watanabe, Yosuke; Hirano, Takayuki; Furuya, Takeshi; Masuko, Takayuki; Ueno, Takahiro; Fukuda, Noboru; Soma, Masayoshi; Ozaki, Toshinori; Koshinaga, Tsugumichi; Nagase, Hiroki

    2015-07-01

    KvDMR (an intronic CpG island within the KCNQ1 gene) is one of the imprinting control regions on human chromosome 11p15.5. Since KvDMR exists within the promoter region of KCNQ1OT1 (antisense transcript of KCNQ1), it is likely that genomic alterations of this region including deletion, paternal uniparental disomy and de-methylation in maternal allele lead to aberrant overexpression of KCNQ1OT1. Indeed, de-methylation of KvDMR accompanied by uncontrolled overexpression of KCNQ1OT1 occurs frequently in Beckwith-Wiedemann syndrome (BWS), and around 10% of BWS patients developed embryonal tumors (Wilms' tumor or hepatoblastoma). These observations strongly suggest that silencing of KCNQ1OT1 expression might suppress its oncogenic potential. In the present study, we designed two pyrrole-imidazole (PI) polyamides, termed PI-a and PI-b, which might have the ability to bind to CCAAT boxes of the KCNQ1OT1 promoter region, and investigated their possible antitumor effect on Wilms' tumor-derived G401 cells. Gel retardation assay demonstrated that PI-a and PI-b specifically bind to their target sequences. Microscopic observations showed the efficient nuclear access of these PI polyamides. Quantitative real-time PCR analysis revealed that the expression level of KCNQ1OT1 was significantly decreased when treated with PI-a and PI-b simultaneously but not with either PI-a or PI-b single treatment. Consistent with these results, the combination of PI-a and PI-b resulted in a significant reduction in viability of G401 cells in a dose-dependent manner. Furthermore, FACS analysis demonstrated that combinatory treatment with PI-a and PI-b induces cell death as compared with control cells. Taken together, our present observations strongly suggest that the combinatory treatment with PI polyamides targeting KCNQ1OT1 might be a novel therapeutic strategy to cure patients with tumors over-expressing KCNQ1OT1. PMID:25998555

  16. DAO spectroscopic classification of MASTER OT J140958.91+174549.4 as a core-collapse supernova.

    NASA Astrophysics Data System (ADS)

    Balam, D. D.; Graham, M. L.

    2016-04-01

    A spectrum was obtained on UT April 30.39 of MASTER OT J140958.91+174549.4 (CSS160427:140959+174549) using the 1.82-m Plaskett telescope (National Research Council of Canada) covering the range 365-710 nm (resolution 0.35 nm). Cross-correlation with a template library using SNID (Blondin & Tonry 2007, ApJ, 666, 1024) shows MASTER OT J140958.91+174549.4 to be a core-collapse supernova near maximum light.

  17. The R package otu2ot for implementing the entropy decomposition of nucleotide variation in sequence data

    PubMed Central

    Ramette, Alban; Buttigieg, Pier Luigi

    2014-01-01

    Oligotyping is a novel, supervised computational method that classifies closely related sequences into “oligotypes” (OTs) based on subtle nucleotide variation (Eren et al., 2013). Its application to microbial datasets has helped reveal ecological patterns which are often hidden by the way sequence data are currently clustered to define operational taxonomic units (OTUs). Here, we implemented the OT entropy decomposition procedure and its unsupervised version, Minimal Entropy Decomposition (MED; Eren et al., 2014c), in the statistical programming language and environment, R. The aim of this implementation is to facilitate the integration of computational routines, interactive statistical analyses, and visualization into a single framework. In addition, two complementary approaches are implemented: (1) An analytical method (the broken stick model) is proposed to help identify OTs of low abundance that could be generated by chance alone and (2) a one-pass profiling (OP) method, to efficiently identify those OTUs whose subsequent oligotyping would be most promising to be undertaken. These enhancements are especially useful for large datasets, where a manual screening of entropy analysis results and the creation of a full set of OTs may not be feasible. The package and procedures are illustrated by several tutorials and examples. PMID:25452747

  18. Spectroscopic Classification of MASTER OT J152333.04+092125.6 as a post-maximum Type Ia Supernova

    NASA Astrophysics Data System (ADS)

    Kilpatrick, Charles; Andrews, Jennifer; Milne, Peter; Smith, Nathan

    2016-04-01

    The AZTEC (Arizona Transient Exploration and Characterization) collaboration reports an optical spectrum of MASTER OT J152333.04+092125.6 (ATEL #8903) obtained on UT 2016 Apr. 27.440 with the 2.3m Bok telescope (+ Boller & Chivens spectrograph) on Kitt Peak, Arizona.

  19. Spectroscopic Classification of AT2016axw (=MASTER OT J142718.40-014031.0 =PS16axi)

    NASA Astrophysics Data System (ADS)

    Blanchard, Peter; Berger, Edo; Bieryla, Allyson; Brzycki, Bryan; Cohn, Amy; Dillett, Sean; Feder-Staehle, Richard; Frostig, Danielle; Grell, Gabe; James, Nicholas; Johnson, April; Mahmood, Zahra; Merryman, Hunter; Peak, Barra; Sanders, Cecilia; Taylor, Anthony

    2016-03-01

    We obtained a low-resolution optical spectrum of AT2016axw (=MASTER OT J142718.40-014031.0 =PS16ayd; ATel #8791). Observations were carried out on 2016 March 16.4 UT using the FAST spectrograph mounted on the F.L. Whipple Observatory 1.5-m telescope.

  20. 12 CFR 502.26 - How does OTS calculate the semi-annual assessment for savings and loan holding companies?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...-site supervision of a noncomplex, low risk savings and loan holding company structure. OTS will... holding company's business, a component based on its organizational form, and a component based on its... company is the registered holding company at the highest level of ownership in a holding company...

  1. 12 CFR 502.26 - How does OTS calculate the semi-annual assessment for savings and loan holding companies?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... reflect OTS costs of supervising the holding company structure and: (i) There are multiple top-tier holding companies in the holding company structure; (ii) The top-tier holding company is organized outside...) Other circumstances indicate that the assessment of the top-tier holding company is inappropriate....

  2. SALT spectral classification of MASTER OT J074858.45-655344.6.5 as a likely Dwarf Nova in outburst

    NASA Astrophysics Data System (ADS)

    Buckley, D. A. H.; Kniazev, A.; Kotze, M. M.; Potter, S.; Gorbovskoy, E.; Lipunov, V.

    2015-03-01

    MASTER OT J074858.45-655344.6.5, which was discovered by MASTER SAAO on Jan. 15.045 UT (ATel #6927) as an unfiltered 16.9 magnitude transient, was observed with SALT RSS from Jan. 15.95 - 15.99 UT using the PG900 lines/mm grating.

  3. Asiago spectroscopic classification of MASTER OT J162655.28+333824.4 as a type-Ia supernova

    NASA Astrophysics Data System (ADS)

    Terreran, G.; Tomasella, L.; Benetti, S.; Cappellaro, E.; Elias-Rosa, N.; Ochner, P.; Pastorello, A.; Turatto, M.

    2014-04-01

    We report that an optical spectrogram of PSN J16265528+3338244 (= MASTER OT J162655.28+333824.4, #6024), 340-820 nm; resolution 1.3 nm, obtained on March 31.98 UT with the Asiago 1.82-m Copernico Telescope (+ AFOSC), shows it to be a type-Ia supernova at redshift around 0.04. ...

  4. Purification, crystallization and preliminary crystallographic analysis of the biotin–protein ligase from Pyrococcus horikoshii OT3

    SciTech Connect

    Bagautdinov, Bagautdin; Kuroishi, Chizu; Sugahara, Mitsuaki; Kunishima, Naoki

    2005-02-01

    The biotin–protein ligase from P. horikoshii OT3 was overexpressed, purified, crystallized and cocrystallized with biotin, ADP and biotinyl-5′-AMP. The crystals belong to space group P2{sub 1} and diffract to beyond 1.6 Å resolution.

  5. 75 FR 63107 - Alternatives to the Use of External Credit Ratings in the Regulations of the OTS

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-14

    ... capital frameworks. (75 FR 52283) II. OTS Regulations Referencing Credit Ratings The non-capital... different regulations? \\8\\ 75 FR 52283, August 25, 2010. Alternatives for Replacing References to Credit... banking agencies), including alternative measures of credit-worthiness that may be used in lieu of...

  6. 12 CFR 563.580 - What standards and procedures will govern OTS review of the substance of my notice?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false What standards and procedures will govern OTS review of the substance of my notice? 563.580 Section 563.580 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY SAVINGS ASSOCIATIONS-OPERATIONS Notice of Change of Director or Senior Executive Officer § 563.580 What...

  7. 75 FR 10560 - Application for Conversion From: (a) OTS-Regulated, State-Chartered Savings Association to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-08

    ... Union to Federal Savings Association; (c) State Mutual Holding Company to a Federal Mutual Holding.... SUMMARY: The proposed information collection request (ICR) described below has been submitted to the... Information and Regulatory Affairs, Attention: Desk Officer for OTS, U.S. Office of Management and Budget,...

  8. The Persistent Eruption of UGC 2773-OT: finally, a decade-long extragalactic Eta Carinae analogue

    NASA Astrophysics Data System (ADS)

    Smith, Nathan; Andrews, Jennifer E.; Mauerhan, Jon C.; Zheng, WeiKang; Filippenko, Alexei V.; Graham, Melissa L.; Milne, Peter

    2016-02-01

    While supernova (SN) impostors resemble the Great Eruption of η Carinae in the sense that their spectra show narrow H lines and they have typical peak absolute magnitudes of -13 to -14 mag, most extragalactic events observed so far are quite different from η Car in duration. Their bright phases typically last for ˜100 d or less, rather than persisting for several years. The transient object UGC 2773-OT (discovered in 2009) had a similar peak absolute magnitude to other SN impostors, but with a gradual 5-yr pre-discovery rise. In the ˜6 yr since discovery, it has faded very slowly (0.26 mag yr-1). Overall, we suggest that its decade-long eruption is so far the best-known analogue of η Car's 19th century eruption. We discuss extensive spectroscopy of the ongoing eruption. The spectra show interesting changes in velocity and line shape that we discuss in detail, including an asymmetric Hα emission line that we show is consistent with the ejection of a bipolar nebula that could be very much like the Homunculus of η Car. Moreover, changes in the line width, line profile, blue excess emission resembling that of Type IIn SNe, and the intensity of Hα suggest the presence of strong circumstellar interaction in the eruption at late times. This supports the hypothesis that the extended plateau of η Car's eruption may have been powered by shock interaction as well. One interesting difference compared to η Car, however, is that UGC 2773-OT so far does not exhibit the repeated brief spikes in luminosity that have been associated with binary periastron events.

  9. Solar energy system performance evaluation: final report for Honeywell OTS 41, Shenandoah (Newnan), Georgia

    SciTech Connect

    Mathur, A K; Pederson, S

    1982-08-01

    The operation and technical performance of the Solar Operational Test Site (OTS 41) located at Shenandoah, Georgia, are described, based on the analysis of data collected between January and August 1981. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 41 is a hydronic heating and cooling system consisting of 702 square feet of liquid-cooled flat-plate collectors; a 1000-gallon thermal storage tank; a 3-ton capacity organic Rankine-cycle-engine-assisted air conditioner; a water-to-air heat exchanger for solar space heating; a finned-tube coil immersed in the storage tank to preheat water for a gas-fired hot water heater; and associated piping, pumps, valves, and controls. The solar system has six basic modes of operation and several combination modes. The system operation is controlled automatically by a Honeywell-designed microprocessor-based control system, which also provides diagnostics. Based on the instrumented test data monitored and collected during the 7 months of the Operational Test Period, the solar system collected 53 MMBtu of thermal energy of the total incident solar energy of 219 MMBtu and provided 11.4 MMBtu for cooling, 8.6 MMBtu for heating, and 8.1 MMBtu for domestic hot water. The projected net annual energy savings due to the solar system were approximately 50 MMBtu of fossil energy (49,300 cubic feet of natural gas) and a loss of 280 kWh(e) of electrical energy.

  10. Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis

    PubMed Central

    Barber, Carmel M.; Ahmad Rusmili, Muhamad Rusdi; Hodgson, Wayne C.

    2016-01-01

    Taipans (Oxyuranus spp.) are elapids with highly potent venoms containing presynaptic (β) and postsynaptic (α) neurotoxins. O. temporalis (Western Desert taipan), a newly discovered member of this genus, has been shown to possess venom which displays marked in vitro neurotoxicity. No components have been isolated from this venom. We describe the characterization of α-elapitoxin-Ot1a (α-EPTX-Ot1a; 6712 Da), a short-chain postsynaptic neurotoxin, which accounts for approximately 30% of O. temporalis venom. α-Elapitoxin-Ot1a (0.1–1 µM) produced concentration-dependent inhibition of indirect-twitches, and abolished contractile responses to exogenous acetylcholine and carbachol, in the chick biventer cervicis nerve-muscle preparation. The inhibition of indirect twitches by α-elapitoxin-Ot1a (1 µM) was not reversed by washing the tissue. Prior addition of taipan antivenom (10 U/mL) delayed the neurotoxic effects of α-elapitoxin-Ot1a (1 µM) and markedly attenuated the neurotoxic effects of α-elapitoxin-Ot1a (0.1 µM). α-Elapitoxin-Ot1a displayed pseudo-irreversible antagonism of concentration-response curves to carbachol with a pA2 value of 8.02 ± 0.05. De novo sequencing revealed the main sequence of the short-chain postsynaptic neurotoxin (i.e., α-elapitoxin-Ot1a) as well as three other isoforms found in O. temporalis venom. α-Elapitoxin-Ot1a shows high sequence similarity (i.e., >87%) with other taipan short-chain postsynaptic neurotoxins. PMID:26938558

  11. Overexpression of the non-coding SOX2OT variants 4 and 7 in lung tumors suggests an oncogenic role in lung cancer.

    PubMed

    Saghaeian Jazi, Marie; Samaei, Nader Mansour; Ghanei, Mostafa; Shadmehr, Mohammad Behgam; Mowla, Seyed Javad

    2016-08-01

    Despite the advances in cancer therapy, lung cancer still remains the most leading cause of cancer death worldwide. The long non-coding RNAs (lncRNAs) are recently introduced as novel regulators of human cancers. SOX2 overlapping transcript (SOX2OT) is a cancer-associated lncRNA gene that encodes different alternatively spliced transcripts. Here, we investigated the alterations in the preferential expression of different SOX2OTs in twenty non-small cell lung cancer (NSCLC) patients by real-time quantitative reverse transcription PCR (qRT-PCR) method. We observed preferential expression of SOX2OT4 and SOX2OT7 in lung tumor tissues. The quantitative gene expression analysis revealed that >30 % of NSCLC tumors express SOX2OT4 (mean = 7.6 times) and SOX2OT7 (mean = 5.9 times) more than normal tissues, with higher expression in squamous cell carcinoma. Further, we observed overexpression of pluripotency-associated transcription factor, SOX2 in 47 % of our samples concordant with SOX2OT (R = 0.62, P value <0.05). Overexpression of OCT4A gene was also observed in 36.8 % of tumor tissues. Then, we investigated the effects of SOX2OT suppression in lung adenocarcinoma cell line, by means of RNAi. Cell characteristics of colony formation, apoptosis, 2-D mobility, and cell cycle progression were measured in control and treated A549 cells. The SOX2OT knockdown significantly reduced the colony formation ability of cancer cells; however, no alterations in the rate of apoptosis were detected. On the other hand, SOX2OT-suppressed cells had elevated accumulation in G2/M phase of cell cycle and exhibited limited mobility. Altogether, our findings support a potential oncogenic role for SOX2OT in non-small cell lung cancer tumor genesis and SOX2OT seems a promising therapeutic candidate for NSCLC. PMID:26846097

  12. Effect of Ozone Therapy (OT) on Healing of Colonic Anastomosis in a Rat Model of Peritonitis

    PubMed Central

    Erginel, Başak; Erginel, Turgay; Aksoy, Bilgin; Dokucu, Ali İhsan

    2014-01-01

    Background: Ozone is a three-oxygen molecule (O3). Ozone therapy (OT) is systematically effective when pathological inflammatory and immunologic processes are activated. Among of these conditions are wound healing, macular degeneration related to aging, and conditions that are ischemic or infectious. Aims: The aim of this study was to determine the effects of OT on wound healing of intestinal anastomosis in the presence of peritonitis in a rat model. Study Design: Animal experimentation. Methods: A total of 40 Wistar albino rats were randomized into four groups (n=10) including: sham (S), peritonitis (P), ozone 0 (O0), and ozone 24 (O24). In group S, only cecal dissection was carried out. The S group had only a cecal dissection and intestinal anastomosis performed, but no peritonitis. In all other groups, cecal ligation and puncture (CLP) followed the cecal dissection to induce bacterial peritonitis. 24 h after puncture, a cecal resection and ileocolic anastomosis were performed. In group P, 24 h after CLP, a cecal resection and ileocolic anastomosis were performed and no ozone was administered. In group O0, immediately after the anastomosis, and in group O24, starting 24 hours after the anastomosis, an intraperitoneal 1 mg/kg/day ozone administration was applied for seven days. On the seventh day the animals were sacrificed, the anastomotic bursting pressures (BP) and the hydroxyproline values of the anastomotic tissues were measured, and histopathologic examination of the anastomotic segment was carried out. Results: The highest BP was in group S, with 211±23.13 mmHg. The mean BP of group P was 141±56.25 mmHg, which was significantly lower than in the other two peritonitis groups that received ozone therapy, group O0 and O24, where it was 192±22 and 166±45 mmHg, respectively (p<0.05). The difference in the BP between groups O0 and O24 was not statistically significant (p>0.05). Histopathologic analyses of the anastomotic segments determined there was

  13. Partial KCNQ1OT1 hypomethylation: A disguised familial Beckwith-Wiedemann syndrome as a sporadic adrenocortical tumor.

    PubMed

    H'mida Ben-Brahim, Dorra; Hammami, Sabeur; Haddaji Mastouri, Marwa; Trabelsi, Saoussen; Chourabi, Maroua; Sassi, Sihem; Mougou, Soumaya; Gribaa, Moez; Zakhama, Abdelfattah; Guédiche, Mohamed Neji; Saad, Ali

    2015-03-01

    Beckwith-Wiedemann syndrome has a wide spectrum of complications such as embryonal tumors, namely adrenocortical tumor. Tumor predisposition is one of the most challenging manifestations of this syndrome. A 45-day old female with a family history of adrenocortical tumor presented with adrenocortical tumor. The case raised suspicion of a hereditary Beckwith-Wiedemann syndrome, therefore molecular analysis was undertaken. The results revealed partial KCNQ1OT1 hypomethylation in the infant's blood DNA which was associated with a complete loss of methylation in the infant's adrenocortical tumor tissue. It is unique for familial Beckwith-Wiedemann syndrome caused by KCNQ1OT1 partial hypomethylation to manifest solely through adrenocortical tumor. Incomplete penetrance and specific tissue mosaicism could provide explanations to this novel hereditary Beckwith-Wiedemann syndrome presentation. PMID:26937341

  14. MASTER OT J130845.02-323254.9: Variable Stars as Source of the High Energy Neutrino.

    NASA Astrophysics Data System (ADS)

    Lipunov, V.; Tyurina, N.; Gorbovskoy, E.; Buckley, D.

    2016-09-01

    As reported in ATel #9425 Global MASTER Net auto-detection system ( ( Lipunov et al., MASTER Global Robotic Net, Advances in Astronomy, 2010, 30L) discovered OT source at (RA, Dec) = 13h 08m 45.02s -32d 32m 54.9s on 2016-08-24.73811 UT during inspection of HESE IceCube alert (14 August 2016, 58537957 trigger number http://gcn.gsfc.nasa.gov/notices_amon/58537957_128340.amon , Dornic et al. ATEL #9440 ). MASTER-SAAO auto-detection system detected again OT at RA (2000) = 13 08 45.02 -32 32 54.9 on 2016-09-04.7627UT (ATEL #9425).

  15. Impaired Memory in OT-II Transgenic Mice Is Associated with Decreased Adult Hippocampal Neurogenesis Possibly Induced by Alteration in Th2 Cytokine Levels.

    PubMed

    Jeon, Seong Gak; Kim, Kyoung Ah; Chung, Hyunju; Choi, Junghyun; Song, Eun Ji; Han, Seung-Yun; Oh, Myung Sook; Park, Jong Hwan; Kim, Jin-Il; Moon, Minho

    2016-08-31

    Recently, an increasing number of studies have focused on the effects of CD4+ T cell on cognitive function. However, the changes of Th2 cytokines in restricted CD4+ T cell receptor (TCR) repertoire model and their effects on the adult hippocampal neurogenesis and memory are not fully understood. Here, we investigated whether and how the mice with restricted CD4+ repertoire TCR exhibit learning and memory impairment by using OT-II mice. OT-II mice showed decreased adult neurogenesis in hippocampus and short- and long- term memory impairment. Moreover, Th2 cytokines in OT-II mice are significantly increased in peripheral organs and IL-4 is significantly increased in brain. Finally, IL-4 treatment significantly inhibited the proliferation of cultured adult rat hippocampal neural stem cells. Taken together, abnormal level of Th2 cytokines can lead memory dysfunction via impaired adult neurogenesis in OT-II transgenic. PMID:27432189

  16. Impaired Memory in OT-II Transgenic Mice Is Associated with Decreased Adult Hippocampal Neurogenesis Possibly Induced by Alteration in Th2 Cytokine Levels

    PubMed Central

    Jeon, Seong Gak; Kim, Kyoung Ah; Chung, Hyunju; Choi, Junghyun; Song, Eun Ji; Han, Seung-Yun; Oh, Myung Sook; Park, Jong Hwan; Kim, Jin-il; Moon, Minho

    2016-01-01

    Recently, an increasing number of studies have focused on the effects of CD4+ T cell on cognitive function. However, the changes of Th2 cytokines in restricted CD4+ T cell receptor (TCR) repertoire model and their effects on the adult hippocampal neurogenesis and memory are not fully understood. Here, we investigated whether and how the mice with restricted CD4+ repertoire TCR exhibit learning and memory impairment by using OT-II mice. OT-II mice showed decreased adult neurogenesis in hippocampus and short- and long- term memory impairment. Moreover, Th2 cytokines in OT-II mice are significantly increased in peripheral organs and IL-4 is significantly increased in brain. Finally, IL-4 treatment significantly inhibited the proliferation of cultured adult rat hippocampal neural stem cells. Taken together, abnormal level of Th2 cytokines can lead memory dysfunction via impaired adult neurogenesis in OT-II transgenic. PMID:27432189

  17. FLOYDS Classification of MASTER OT J164244.87+272054.3 as a Type Ia Supernova Near Peak

    NASA Astrophysics Data System (ADS)

    Hosseinzadeh, G.; Arcavi, I.; Howell, D. A.; McCully, C.; Valenti, S.

    2016-05-01

    We obtained a spectrum of MASTER OT J164244.87+272054.3 (ATel #9022) on 2016 May 6.6 UT with the robotic FLOYDS instrument mounted on the LCOGT 2-meter telescope in Siding Spring, Australia. Using SNID (Blondin & Tonry 2007, ApJ, 666, 1024), we find a good fit to the normal Type Ia SN 1990N 2 days after maximum light at redshift z=0.108.

  18. OT2_peisenha_2: The Most Luminous Obscured Galaxies and Quasars Revealed by WISE and Herschel

    NASA Astrophysics Data System (ADS)

    Eisenhardt, P.

    2011-09-01

    NASA's Wide-field Infrared Survey Explorer (WISE) has surveyed the entire sky at 3.4, 4.6, 12 and 22 microns (W1, W2, W3, and W4), reaching sensitivities hundreds of times deeper than IRAS. We have used WISE photometry to select an all-sky sample of objects which are extremely luminous. The objects are prominent in W4, but faint or undetected in W1 and W2 (W12drops). Followup spectroscopy of ~ 100 sources shows over 70% of W12drops have redshifts > 1.6, which with OT1 PACS and SPIRE photometry of 27 sources leads to over 1E13 solar luminosities, with ~ 10% exceeding 1E14 solar luminosities. High resolution adaptive optics imaging shows these objects are unlensed. We request 47.3 hours of Herschel time to complete the all-sky sample of the brightest 185 W12 drops, fulfilling the primary WISE objective of finding the most extreme luminous IR galaxies in the Universe. These superlative objects will be the most fruitful for detailed studies of the physics of star formation, AGN fueling, and feedback in the most active galaxies.

  19. Synthesis and characterization of nanodispersed molecular aggregates of Prussian blue in aerosol OT reverse micelle.

    PubMed

    Pramanik, Smritimoy; Das, Debasmita; Das, Kaushik; Bhattacharya, Subhash Ch

    2007-02-01

    Prussian Blue (PB) nanomolecular aggregates were prepared in a well-characterized, monodispersed biomimicking nanocavities formed by Aerosol OT (AOT) reverse micelle in H2O/AOT/heptane at different omega ([H2O]/[Surfactant]) employing coprecipitation technique. The formed nanomolecular aggregates of PB have been characterized by the UV-visible, Fourier Transformed Infrared (FTIR) spectroscopy as well as by Transmission Electron Microscopy (TEM) and Cyclic Voltammetric methods. Visible and FTIR spectroscopic measurements confirm the formation of PB nano aggregates. Experimental results reveal that the molar extinction coefficient of PB nanomolecular aggregates is different for two different regimes of omega of reverse micelles. TEM measurements show that the size of these reverse micellar entrapped nano aggregrates varied with hydration (omega). Studies on these nano sized particles indicate that Fe is present in a single mixed valence state along the Fe-C-N-Fe skeleton in PB and the half wave potential (E1/2) becomes more positive with increase in the size of the nano aggregates. PMID:17450811

  20. Aerosol-OT-gamma-alumina admicelles for the concentration of hydrophobic organic compounds in water.

    PubMed

    Saitoh, Tohru; Matsushima, Seiichi; Hiraide, Masataka

    2004-06-25

    A novel admicelle composing of a dialkylated anionic surfactant, di-2-ethylhexyl sodium sulfosuccinate (Aerosol-OT, AOT) and gamma-alumina was prepared by mixing them in acidic aqueous solution. The amount of the maximum sorption of AOT on 1 g of alumina at pH 2 was ca. 130 mg. By comparing the fluorescence spectra of N-phenyl-1-naphthylamine in different solvents, the solvent property of AOT-gamma-alumina admicelles was corresponding to that of toluene or diethyl ether. Thus, the AOT-gamma-alumina admicelles had greater hydrophobicity than SDS-gamma-alumina admicelles having similar hydrophobicity to 1-octanol or ethyl acetate. Hydrophobic organic compounds, chlorophenols having more than three chloro substituents, octylphenol, nonylphenol, dibutyl phthalate was almost quantitatively (98% or more) collected onto AOT admicelles composing of 1.5 g gamma-alumina and 150 mg AOT. The greater collection yields rather than those in SDS-admicellar system were ascribable to greater hydrophobicity and stability of AOT admicelles. After the 500-fold concentration, traces (nM) of organic contaminants in water samples were successfully detected with an HPLC having a photometric detector. PMID:15230525

  1. Rotational reorientation dynamics of Aerosol-OT reverse micelles formed in near-critical propane

    SciTech Connect

    Heitz, M.P.; Bright, F.V.

    1996-06-01

    The rotational reorientation kinetics of two fluorescent solutes (rhodamine 6G, R6G, and rhodamine 101, R101) have been determined in sodium bis(2-ethylhexyl) sulfosuccinate (Aerosol-OT, AOT) reverse micelles formed in liquid and near-critical propane. We show that the amount of water loading ([water]/[AOT], R), continuous phase density, and temperature all influence the solute rotational dynamics. In all cases, the decay of anisotropy data (i.e., frequency-dependent differential polarized phase angle and polarized modulation ratio) are well described by a bi-exponential decay law. We find that the faster rotational correlation times are similar to but slightly less than the values predicted for an individual AOT reverse micelle rotating in propane. The recovered rotational correlation times range from 200 to 500 ps depending on experimental conditions. This faster rotational process is explained in terms of lateral diffusion of the fluorophore along the water/headgroup interfacial region within the reverse micelle. The recovered values for the slower rotational correlation times range from 7 to 18 ns. These larger rotational reorientation times are assigned to varying micelle-micelle (i.e., tail-tail) interactions in the low-density, highly compressible fluid region. We also quantify the contribution of the reverse micellar {open_quotes}aggregate{close_quotes} to the total decay of anisotropy. {copyright} {ital 1996} {ital Society for Applied Spectroscopy}

  2. Almost Gone: SN 2008S and NGC 300 2008OT-1 are Fainter than their Progenitors

    NASA Astrophysics Data System (ADS)

    Adams, S. M.; Kochanek, C. S.; Prieto, J. L.; Dai, X.; Shappee, B. J.; Stanek, K. Z.

    2016-05-01

    We present late-time Hubble and Spitzer Space Telescope imaging of SN 2008S and NGC 300 2008OT-1, the prototypes of a common class of stellar transients whose true nature is debated. Both objects are still fading and are now >15 times fainter than their progenitors in the mid-IR and are undetected in the optical and near-IR. Data from the Large Binocular Telescope and Magellan show that neither source has been variable in the optical since fading in 2010. We present models of surviving sources obscured by dusty shells or winds and find that extreme dust models are needed for surviving stars to be successfully hidden by dust, which suggests that these transients may be genuine, but low energy, supernova explosions. Though SN 2008S is not detected in Chandra X-Ray Observatory data taken in 2012, the flux limits allow the fading IR source to be powered solely by the shock interaction of ejecta with the circumstellar medium if the shock velocity at the time of the observation was ≳20% slower than estimated from emission line widths while the transient was still optically bright. Continued SST monitoring and 10 - 20 μm observations with JWST can resolve any remaining ambiguities.

  3. The crystal structure of Z-Gly-Aib-Gly-Aib-OtBu.

    PubMed

    Gessmann, Renate; Brückner, Hans; Aivaliotis, Michalis; Petratos, Kyriacos

    2015-06-01

    The synthetic peptide Z-Gly-Aib-Gly-Aib-OtBu was dissolved in methanol and crystallized in a mixture of ethyl acetate and petroleum ether. The crystals belong to the centrosymmetric space group P4/n that is observed less than 0.3% in the Cambridge Structural Database. The first Gly residue assumes a semi-extended conformation (φ ±62°, ψ ∓131°). The right-handed peptide folds in two consecutive β-turns of type II' and type I or an incipient 310 -helix, and the left-handed counterpart folds accordingly in the opposite configuration. In the crystal lattice, one molecule is linked to four neighbors in the ab-plane via hydrogen bonds. These bonds form a continuous network of left- and right-handed molecules. The successive ab-planes stack via apolar contacts in the c-direction. An ethyl acetate molecule is situated on and close to the fourfold axis. PMID:25781150

  4. Almost gone: SN 2008S and NGC 300 2008OT-1 are fainter than their progenitors

    NASA Astrophysics Data System (ADS)

    Adams, S. M.; Kochanek, C. S.; Prieto, J. L.; Dai, X.; Shappee, B. J.; Stanek, K. Z.

    2016-08-01

    We present late-time Hubble and Spitzer Space Telescope (SST) imaging of SN 2008S and NGC 300 2008OT-1, the prototypes of a common class of stellar transients whose true nature is debated. Both objects are still fading and are now >15 times fainter than their progenitors in the mid-IR and are undetected in the optical and near-IR. Data from the Large Binocular Telescope and Magellan show that neither source has been variable in the optical since fading in 2010. We present models of surviving sources obscured by dusty shells or winds and find that extreme dust models are needed for surviving stars to be successfully hidden by dust, which suggests that these transients may be genuine, but low-energy, supernova explosions. Though SN 2008S is not detected in Chandra X-Ray Observatory data taken in 2012, the flux limits allow the fading IR source to be powered solely by the shock interaction of ejecta with the circumstellar medium if the shock velocity at the time of the observation was ≳20 per cent slower than estimated from emission line widths while the transient was still optically bright. Continued SST monitoring and 10-20 μm observations with James Webb Space Telescope can resolve any remaining ambiguities.

  5. Molecular characterization of the group II chaperonin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3.

    PubMed

    Okochi, Mina; Matsuzaki, Hiroki; Nomura, Tomoko; Ishii, Noriyuki; Yohda, Masafumi

    2005-04-01

    The group II chaperonin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3 (PhCPN) and its functional cooperation with the cognate prefoldin were investigated. PhCPN existed as a homo-oligomer in a double-ring structure, which protected the citrate synthase of a porcine heart from thermal aggregation at 45 degrees C, and did the same on the isopropylmalate dehydrogenase (IPMDH) of a thermophilic bacterium, Thermus thermophilus HB8, at 90 degrees C. PhCPN also enhanced the refolding of green fluorescent protein (GFP), which had been unfolded by low pH, in an ATP-dependent manner. Unexpectedly, functional cooperation between PhCPN and Pyrococcus prefoldin (PhPFD) in the refolding of GFP was not observed. Instead, cooperation between PhCPN and PhPFD was observed in the refolding of IPMDH unfolded with guanidine hydrochloride. Although PhCPN alone was not effective in the refolding of IPMDH, the refolding efficiency was enhanced by the cooperation of PhCPN with PhPFD. PMID:15538645

  6. Limited Production (LP) Precision Runway Monitor (PRM) Operational Test and Evaluation integration and OT and E Operational Test Plan

    NASA Astrophysics Data System (ADS)

    Livings, Jeffrey

    1995-05-01

    This document defines the Test Plan and corresponding Test Verification Requirements Traceability Matrix (TVRTM) that will be used to conduct the Limited Production (LP) Precision Runway Monitor (PRM) Operational Test and Evaluation (OT and E) Integration and OT and E Operational tests. These tests will be conducted at the Minneapolis-St. Paul International Airport following the Contractor Site Acceptance Test. The LP PRM OT and E test effort will concentrate on Operational Effectiveness and Suitability. The Operational Effectiveness Test consists of a review of the contractor performed Development Test and Evaluation (DT and E) and Site Acceptance Tests. This review will evaluate whether each of the Measures of Effectiveness had been satisfactorily tested and whether the results meet the Minimum Acceptable Operational REquirements MAORs). This review will be conducted solely by test engineers and does not require the PRM system. The Operational Suitability Tests will expose the test participants (Air Traffic (AT) Controllers and Airway Facilities (AF) Technicians) to the PRM system in an operational environment while they perform specified operational procedures. These tests will be conducted in two separate phases: AT Suitability and AF Suitability. Each of these phases is focused on the specific test participants.

  7. Rice OVERLY TOLERANT TO SALT 1 (OTS1) SUMO protease is a positive regulator of seed germination and root development.

    PubMed

    Srivastava, Anjil Kumar; Zhang, Cunjin; Sadanandom, Ari

    2016-05-01

    Salinity is one of the major environmental stresses affecting rice production worldwide. Improving rice salt tolerance is a critical step for sustainable food production. Posttranslational modifications of proteins greatly expand proteome diversity, increase functionality and allow quick responses to environmental stresses, all at low cost to the cell. SUMO mediated modification of substrate proteins is a highly dynamic process governed by the balance of activities of SUMO E3 ligases and deconjugating SUMO proteases. In recent years, SUMO (Small Ubiquitin like Modifier) conjugation of proteins has emerged as an influential regulator of stress signaling in the model plant Arabidopsis. However SUMOylation remain largely under studied in crop plants. We recently identified the SUMO protease gene family in rice and demonstrated a role for OsOTS1 SUMO proteases in salt stress. Interestingly, rice plants silencing OsOTS1 also show significantly reduced germination rate. Knockdown of OsOTS1 gene expression affects root growth by primarily reducing cell size rather than cell division. PMID:27119209

  8. Effect of concentration and addition of ions on the adsorption of aerosol-OT to sapphire.

    PubMed

    Hellsing, Maja S; Rennie, Adrian R; Hughes, Arwel V

    2010-09-21

    Aerosol-OT (sodium bis 2-ethylhexyl sulfosuccinate or NaAOT) adsorbs to hydrophilic sapphire solid surfaces. The structure of the formed bilayer has been determined over the concentration range 0.2-7.4 mM NaAOT. It was found that the hydrocarbon tails pack at maximum packing limit at very low concentrations, and that the thickness of the bilayer was concentration-independent. The adsorption was found to increase with concentration, with the surfactant molecules packing closer laterally. The area per molecule was found to change from 138 ± 25 to 51 ± 4 A(2) over the concentration range studied, with the thickness of the layer being constant at 33 ± 2 A. Addition of small amounts of salt was found to increase the surface excess, with the bilayer being thinner with a slightly larger area per molecule. Addition of different salts of the same valency was found to have a very similar effect, as had the addition of NaOH and HCl. Hence, the effects of adding acid or base should be considered an effect of ionic strength rather than an effect of pH. Adsorption of NaAOT to the sapphire surface that carries an opposite charge to the anionic surfactant is similar in many respects to the adsorption reported previously for hydrophilic and hydrophobic silica surfaces. This suggests that the adsorption of NaAOT to a surface is driven primarily by NaAOT self-assembly rather than effects of electrostatic attraction to the interface. PMID:20735040

  9. Molecular simulation study of water mobility in aerosol-OT reverse micelles.

    PubMed

    Chowdhary, Janamejaya; Ladanyi, Branka M

    2011-06-16

    In this work, we present results from molecular dynamics simulations on the single-molecule relaxation of water within reverse micelles (RMs) of different sizes formed by the surfactant aerosol-OT (AOT, sodium bis(2-ethylhexyl)sulfosuccinate) in isooctane. Results are presented for RM water content w(0) = [H(2)O]/[AOT] in the range from 2.0 to 7.5. We show that translational diffusion of water within the RM can, to a good approximation, be decoupled from the translation of the RM through the isooctane solvent. Water translational mobility within the RM is restricted by the water pool dimensions, and thus, the water mean-squared displacements (MSDs) level off in time. Comparison with models of diffusion in confined geometries shows that a version of the Gaussian confinement model with a biexponential decay of correlations provides a good fit to the MSDs, while a model of free diffusion within a sphere agrees less well with simulation results. We find that the local diffusivity is considerably reduced in the interfacial region, especially as w(0) decreases. Molecular orientational relaxation is monitored by examining the behavior of OH and dipole vectors. For both vectors, orientational relaxation slows down close to the interface and as w(0) decreases. For the OH vector, reorientation is strongly affected by the presence of charged species at the RM interface and these effects are especially pronounced for water molecules hydrogen-bonded to surfactant sites that serve as hydrogen-bond acceptors. For the dipole vector, orientational relaxation near the interface slows down more than that for the OH vector due mainly to the influence of ion-dipole interactions with the sodium counterions. We investigate water OH and dipole reorientation mechanisms by studying the w(0) and interfacial shell dependence of orientational time correlations for different Legendre polynomial orders. PMID:21548627

  10. Unique effects of aerosol OT lamellar structures on the dynamics of guest molecules.

    PubMed

    De, Dipanwita; Datta, Anindya

    2013-06-25

    The behavior of lamellar structures of Aerosol OT (AOT) as hosts, vis-à-vis the flexible normal micelles and rigid nanochannels of Nafion membranes, has been investigated with two different fluorophores, [2,2'-bipyridyl]-3,3'-diol (BP(OH)2) and coumarin 102 (C102). Surprisingly, for BP(OH)2, a rise time is observed at intermediate emission wavelengths and not in the red edge of the fluorescence spectrum. A shoulder at 525 nm is observed in time resolved emission spectra (TRES) at initial times of BP(OH)2 in AOT lamellar structures. This feature is the signature of the monoketo (MK) tautomer, observed for the first time in a microheterogeneous medium. Also, the usually ultrafast single proton transfer in BP(OH)2 is retarded to an considerable extent in lamellar structures. The potential of this medium in promoting unusual intermediates is thus highlighted. This property may be ascribed to the rigidity of lamellar structures, compared to hosts such as regular micelles. However, studies using another fluorophore, coumarin 102 (C102), brings out the fact that these structures are significantly different from the rigid host, Nafion, as well. The absence of excited state proton transfer (ESPT) in this molecule in AOT lamellar structures indicates that it is not protonated, unlike in Nafion. Thus, the interfacial pH of lamellar structures is found to be significantly greater than that of Nafion nanochannels. From the time dependent Stokes shift (TDSS) of the emission spectra of C102, the relaxation time (0.85 ns) of interfacial water in lamellar structures is found to be an order of magnitude faster than that observed in Nafion nanochannels, in which H3O(+) ions have been substituted by different cations. Hence, this study demonstrates that AOT lamellar structures are rather unique hosts and that they behave very differently from conventional rigid and flexible hosts such as normal micelles and Nafion, respectively. PMID:23713719

  11. Whither thou goest? Report of the AOTA Task Force on OT/PT issues.

    PubMed

    Huss, A J

    1984-02-01

    The Task Force on OT/PT Issues through its recommendations attempted to raise to the conscious level some problems that for too long lay hidden or ignored. Most of the Task Force's recommendations have been appropriately channeled for completion by a specified date. Many of the issues identified are not easy ones with which to deal. The professional involvement of all of us is needed to resolve these issues. We, as individuals, need to evaluate objectively our own daily practice about whether or not it falls within the parameters of the official statements given in the Philosophical Base, Principles of Ethics, Standards of Practice, Educational Essentials, and so forth. We cannot blithely continue to wait for the AOTA to do something about it. We are the AOTA! And each of us must do his or her part. An organization and a profession are only as strong as each individual who claims to be a part of it. I believe very strongly in both occupational therapy and physical therapy. I believe that each profession has a unique contribution to make to the client. I believe these contributions are separate but enhancing-if we dilute them the patient/client is the loser. One person cannot be all things to a patient/client. It takes the combined expertise of many specialists to meet the patients'/clients' needs. Each of us is an ambassador of the profession we represent. Each of us, therefore, has an obligation to be a positive representative in all that we do and to responsibly and ethically influence the direction of the profession. Only you and I in our daily professional responsibilities can determine whither thou goest. PMID:6711664

  12. Discovery of Precursor Luminous Blue Variable Outbursts in Two Recent Optical Transients: The Fitfully Variable Missing Links UGC 2773-OT and SN 2009ip

    NASA Astrophysics Data System (ADS)

    Smith, Nathan; Miller, Adam; Li, Weidong; Filippenko, Alexei V.; Silverman, Jeffrey M.; Howard, Andrew W.; Nugent, Peter; Marcy, Geoffrey W.; Bloom, Joshua S.; Ghez, Andrea M.; Lu, Jessica; Yelda, Sylvana; Bernstein, Rebecca A.; Colucci, Janet E.

    2010-04-01

    We present progenitor-star detections, light curves, and optical spectra of supernova (SN) 2009ip and the 2009 optical transient in UGC 2773 (U2773-OT), which were not genuine SNe. Precursor variability in the decade before outburst indicates that both of the progenitor stars were luminous blue variables (LBVs). Their pre-outburst light curves resemble the S Doradus phases that preceded giant eruptions of the prototypical LBVs η Carinae and SN 1954J (V12 in NGC 2403), with intermediate progenitor luminosities. Hubble Space Telescope detections a decade before discovery indicate that the SN 2009ip and U2773-OT progenitors were supergiants with likely initial masses of 50-80 M sun and gsim20 M sun, respectively. Both outbursts had spectra befitting known LBVs, although in different physical states. SN 2009ip exhibited a hot LBV spectrum with characteristic speeds of 550 km s-1, plus evidence for faster material up to 5000 km s-1, resembling the slow Homunculus and fast blast wave of η Carinae. In contrast, U2773-OT shows a forest of narrow absorption and emission lines comparable to that of S Dor in its cool state, plus [Ca II] emission and an infrared excess indicative of dust, similar to SN 2008S and the 2008 optical transient in NGC 300 (N300-OT). The [Ca II] emission is probably tied to a dusty pre-outburst environment, and is not a distinguishing property of the outburst mechanism. The LBV nature of SN 2009ip and U2773-OT may provide a critical link between historical LBV eruptions, while U2773-OT may provide a link between LBVs and the unusual dust-obscured transients SN 2008S and N300-OT. Future searches will uncover more examples of precursor LBV variability of this kind, providing key clues that may help unravel the instability driving LBV eruptions in massive stars.

  13. DISCOVERY OF PRECURSOR LUMINOUS BLUE VARIABLE OUTBURSTS IN TWO RECENT OPTICAL TRANSIENTS: THE FITFULLY VARIABLE MISSING LINKS UGC 2773-OT AND SN 2009ip

    SciTech Connect

    Smith, Nathan; Miller, Adam; Li Weidong; Filippenko, Alexei V.; Silverman, Jeffrey M.; Howard, Andrew W.; Marcy, Geoffrey W.; Bloom, Joshua S.; Nugent, Peter; Ghez, Andrea M.; Lu, Jessica; Yelda, Sylvana; Bernstein, Rebecca A.; Colucci, Janet E.

    2010-04-15

    We present progenitor-star detections, light curves, and optical spectra of supernova (SN) 2009ip and the 2009 optical transient in UGC 2773 (U2773-OT), which were not genuine SNe. Precursor variability in the decade before outburst indicates that both of the progenitor stars were luminous blue variables (LBVs). Their pre-outburst light curves resemble the S Doradus phases that preceded giant eruptions of the prototypical LBVs {eta} Carinae and SN 1954J (V12 in NGC 2403), with intermediate progenitor luminosities. Hubble Space Telescope detections a decade before discovery indicate that the SN 2009ip and U2773-OT progenitors were supergiants with likely initial masses of 50-80 M {sub sun} and {approx}>20 M {sub sun}, respectively. Both outbursts had spectra befitting known LBVs, although in different physical states. SN 2009ip exhibited a hot LBV spectrum with characteristic speeds of 550 km s{sup -1}, plus evidence for faster material up to 5000 km s{sup -1}, resembling the slow Homunculus and fast blast wave of {eta} Carinae. In contrast, U2773-OT shows a forest of narrow absorption and emission lines comparable to that of S Dor in its cool state, plus [Ca II] emission and an infrared excess indicative of dust, similar to SN 2008S and the 2008 optical transient in NGC 300 (N300-OT). The [Ca II] emission is probably tied to a dusty pre-outburst environment, and is not a distinguishing property of the outburst mechanism. The LBV nature of SN 2009ip and U2773-OT may provide a critical link between historical LBV eruptions, while U2773-OT may provide a link between LBVs and the unusual dust-obscured transients SN 2008S and N300-OT. Future searches will uncover more examples of precursor LBV variability of this kind, providing key clues that may help unravel the instability driving LBV eruptions in massive stars.

  14. OT2_eegami_6: SPIRE Snapshot Survey II: Using SPT/CODEX Massive Clusters as Powerful Gravitational Lenses

    NASA Astrophysics Data System (ADS)

    Egami, E.

    2011-09-01

    On the extragalactic side, one of the most remarkable results coming out of Herschel is the discovery of extremely bright (>100 mJy in the SPIRE bands) gravitationally lensed galaxies. The great sensitivity and mapping speed of SPIRE have enabled us to find these rare extraordinary objects. What is truly exciting about these bright lensed galaxies is that they enable a variety of detailed multi-wavelength follow-up observations, shedding new light on the physical properties of these high-redshift sources. In this regard, our OT1 program, "SPIRE Snapshot Survey of Massive Galaxy Clusters" turned out to be a great success. After imaging ~50 galaxies out of 279 in the program, we have already found two spectacularly bright lensed galaxies, one of which is at a redshift of 4.69. This type of cluster-lensed sources are not only bright but also spatially stretched over a large scale, so ALMA (or NOEMA in the north) is likely to be able to study them at the level of individual GMCs. Such studies will open up a new frontier in the study of high-redshift galaxies. Here, we propose to extend this highly efficient and effective survey of gravitationally lensed galaxies to another 353 clusters carefully chosen from the SPT and CODEX cluster samples. These samples contain newly discovered high-redshift (z>0.3) massive (>3-4e14 Msun) clusters, which can be used as powerful gravitational lenses to magnify sources at high redshift. With the OT1 and OT2 surveys together, we expect to find ~20 highly magnified SPIRE sources with exceptional brightnesses (assuming a discovery rate of ~1/30). Such a unique sample of extraordinary objects will enable a variety of follow-up sciences, and will therefore remain as a great legacy of the Herschel mission for years to come.

  15. Fermi and Swift observations of correlated outburst activity from the BL Lac object OT 081 (PKS 1749+096)

    NASA Astrophysics Data System (ADS)

    Ciprini, Stefano; Gonzalez, Josefa Becerra; Pivato, Giovanna; Thompson, David J.

    2016-07-01

    We report a strong multi-wavelength outburst in the BL Lac object OT 081 (also known as PKS 1749+096, 4C +09.57) with the radio counterpart position R.A.: 267.88674 deg, Dec.: 9.65020 deg (J2000.0, Lanyi et al. 2010, AJ, 139, 1695) and with redshift z=0.322 (Stickel, Fried, Kuehr 1988, A & A, 191, 16). Gamma ray and X-ray flares reached peaks during the interval 2016 July 16 and 20 simultaneous with an optical outburst (Balonek et al, 2016, ATel #9259).

  16. Fermi-LAT detection of a GeV gamma-ray flare from the BL Lac object OT 081

    NASA Astrophysics Data System (ADS)

    Becerra Gonzalez, J.; Thompson, D.; Fermi-LAT Collaboration

    2016-07-01

    The Large Area Telescope (LAT), one of the two instruments on the Fermi Gamma-ray Space Telescope, has observed increasing gamma-ray flux from a source positionally consistent with the BL Lac object OT 081 (also known as PKS 1749+096, 4C 09.57, 0FGL J1751.5+0935, 1FGL J1751.5+0937, 2FGL J1751.5+0938, 1FHL J1751.5+0938, 3FGL J1751.5+0939), with the radio counterpart position R.A.: 267.88674 deg, Dec.: 9.65020 deg (J2000.0, Lanyi et al. 2010, AJ, 139, 1695).

  17. OT2_dbrisbin_1: A z=1-2 oxygen survey. II. PAH-selected star forming and AGN sources

    NASA Astrophysics Data System (ADS)

    Brisbin, D.

    2011-09-01

    We are conducting a survey of the [CII] 158um line from galaxies at redshifts 1-2 using our grating spectrometer, ZEUS on the CSO. Our first 13 galaxy survey showed that luminous star forming galaxies in this epoch have moderate intensity kpc-scale star formation likely an extension of the Schmidt-Kennicutt law to very high gas mass fractions. Our AGN dominated systems have similarly large scale, but significantly more intense star formation suggesting punctuated, collision-induced star formation. We were awarded OT1 PACS spectroscopy and PACS/SPIRE photometry of these sources to observe the oxygen [OI], [OIII], and [OIV] fine-structure lines and far-IR continuum to characterize the star formation and AGN activity in these sources. Only two of our sources have been observed to date, but with good astrophysical success. Since the OT1 submission, we have detected 11 more z ~1-2 sources in [CII] with ZEUS. Here we propose an OT2 oxygen line/far-IR continuum study for 10 of these new sources. The new source list significantly enhances our OT1 survey in that (1) we nearly double our sample greatly increasing statistical significance of the results (2) the new group includes 7 Spitzer/PAH sources. PAH emission arises from PDRs tracing the photo-electric heating, while the [CII] and [OI] lines trace the cooling. PAHS therefore trace star formation and, since the features are extremely bright, are excellent redshift indicators. Future missions (e.g. JWST and SPICA/SAFARI) will rely on PAH spectroscopy at high z. It is therefore vital to study PAH emission and its relationship to star formation. The proposed work explores this connection at redshifts 1-2, near the peak of star formation per unit co-moving volume over cosmic time. In this interval ZEUS and PACs share a great synergy with well-matched sensitivities enabling detections of [CII] and oxygen in a wide variety of systems.

  18. Purification, crystallization and preliminary crystallographic analysis of the vacuole-type ATPase subunit E from Pyrococcus horikoshii OT3

    SciTech Connect

    Lokanath, Neratur K.; Ukita, Yoko; Sugahara, Mitsuaki; Kunishima, Naoki

    2005-01-01

    The E subunit of vacuole-type ATPase from P. horikoshii OT3 was overexpressed, purified and crystallized. The native crystals diffracted X-rays to 1.85 Å resolution. The vacuole-type ATPases in eukaryotic cells translocate protons across various biological membranes including the vacuolar membrane by consuming ATP molecules. The E subunit of the multisubunit complex V-ATPase from Pyrococcus horikoshii OT3, which has a molecular weight of 22.88 kDa, has been cloned, overexpressed in Escherichia coli, purified and crystallized by the microbatch method using PEG 4000 as a precipitant at 296 K. A data set to 1.85 Å resolution with 98.8% completeness and an R{sub merge} of 6.5% was collected from a single flash-cooled crystal using synchrotron radiation. The crystal belonged to the orthorhombic space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 52.196, b = 55.317, c = 77.481 Å, and is most likely to contain one molecule per asymmetric unit.

  19. Purification, crystallization and preliminary crystallographic analysis of RecA superfamily ATPase PH0284 from Pyrococcus horikoshii OT3

    SciTech Connect

    Bagautdinov, Bagautdin; Kunishima, Naoki

    2006-04-01

    RecA superfamily ATPase PH0284 from P. horikoshii OT3 was overexpressed, purified, crystallized and cocrystallized with ATP. Both crystal forms belong to the trigonal space group P3{sub 2}21 and diffract X-rays to 2.0 and 2.3 Å resolution, respectively. Circadian (daily) protein clocks are found in cyanobacteria, where a complex of the KaiA, KaiB and KaiC proteins generates circadian rhythms. The 28.09 kDa KaiC homologue PH0284 protein from Pyrococcus horikoshii OT3 was cloned and expressed and the purified protein was crystallized by the oil-microbatch method at 295 K. X-ray diffraction data from the crystal were collected to 2.0 Å resolution using synchrotron radiation at 100 K. The crystal belongs to the trigonal space group P3{sub 2}21, with unit-cell parameters a = b = 96.06, c = 298.90 Å. Assuming the presence of one hexamer in the asymmetric unit gives a V{sub M} value of 2.36 Å{sup 3} Da{sup −1} and a solvent content of 47.9%. A cocrystal with ATP was prepared and a diffraction data set was collected at 2.3 Å resolution.

  20. Purification, crystallization and preliminary crystallographic analysis of archaeal 6-pyruvoyl tetrahydrobiopterin synthase homologue PH0634 from Pyrococcus horikoshii OT3

    SciTech Connect

    Bagautdinov, Bagautdin; Sugahara, Mitsuaki; Kunishima, Naoki

    2007-01-01

    An archaeal 6-pyruvoyl tetrahydrobiopterin synthase homologue from P. horikoshii OT3 was overexpressed as native and selenomethionine-substituted protein, purified and crystallized. The native and selenomethionine-derivative crystals are isomorphous and diffract X-rays to 2.1 and 2.9 Å resolution, respectively. 6-Pyruvoyl tetrahydrobiopterin synthase (PTPS) catalyses the conversion of dihydroneopterin triphosphate to 6-pyruvoyl tetrahydropterin, the second of the three enzymatic steps in the synthesis of tetrahydrobiopterin from GTP. PH0634, a 13.51 kDa archaeal PTPS homologue from Pyrococcus horikoshii OT3, was overexpressed as native and selenomethionine-substituted protein and the purified protein was crystallized by the oil-microbatch method at 295 K. X-ray diffraction data were collected to 2.1 Å resolution from the native crystal using synchrotron radiation at 100 K. The crystal belongs to the orthorhombic space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 35.83, b = 95.71, c = 105.65 Å. Threefold noncrystallographic symmetry was identified from self-rotation calculations. Assuming the presence of a trimer in the asymmetric unit, the solvent content is 45% (V{sub M} = 2.24 Å{sup 3} Da{sup −1}). The selenomethionine-substituted crystal is isomorphous to the native crystal and diffracts X-rays to 2.9 Å.

  1. Liquid-crystalline and microemulsion phase behavior in alcohol-free aerosol-OT/oil/brine systems

    SciTech Connect

    Ghosh, O.; Miller, C.A.

    1987-08-13

    The phase behavior of systems containing the pure anionic surfactant Aerosol OT or sodium bis(2-ethylhexyl) sulfosuccinate was studied as a function of salt concentration, surfactant concentration, alkane carbon number, and water-to-oil ratio. Since the hydrophilic and lipophilic properties of Aerosol OT are nearly balanced, the surfactant forms microemulsions with water and oil in the absence of cosurfactant, allowing for simplified representation of phase behavior. In particular, this property aided in the understanding of transitions between the aqueous surfactant phase behavior and the well-studied oil-rich microemulsion regime. With the addition of salt to dilute alcohol-free surfactant-water mixtures, transitions in the liquid-crystalline phases similar to those seen previously for systems containing petroleum sulfonates and other anionic surfactants with alcohol cosurfactants were found. When hydrocarbons of various chain lengths were equilibrated with the aqueous surfactant solutions, again behavior similar to that of anionic surfactants with alcohol cosurfactants was observed. Pseudoternary diagrams of surfactant-brine-oil were constructed at various brine salinities with n-dodecane as the oil. The assumption that brine acts as a pseudocomponent was found to work best at salinities well below and well above the optimum and at low surfactant concentrations. In any case, the results provide extensive information on phase behavior of a four-component system containing a pure anionic surfactant, a pure hydrocarbon, and sodium chloride brine over a region of considerable interest for enhanced oil recovery and other applications.

  2. Anti-Tumor Effects after Adoptive Transfer of IL-12 Transposon-Modified Murine Splenocytes in the OT-I-Melanoma Mouse Model

    PubMed Central

    Foster, Aaron E.; Huye, Leslie; Bear, Adham; Rooney, Cliona M.; Wilson, Matthew H.

    2015-01-01

    Adoptive transfer of gene modified T cells provides possible immunotherapy for patients with cancers refractory to other treatments. We have previously used the non-viral piggyBac transposon system to gene modify human T cells for potential immunotherapy. However, these previous studies utilized adoptive transfer of modified human T cells to target cancer xenografts in highly immunodeficient (NOD-SCID) mice that do not recapitulate an intact immune system. Currently, only viral vectors have shown efficacy in permanently gene-modifying mouse T cells for immunotherapy applications. Therefore, we sought to determine if piggyBac could effectively gene modify mouse T cells to target cancer cells in a mouse cancer model. We first demonstrated that we could gene modify cells to express murine interleukin-12 (p35/p40 mIL-12), a transgene with proven efficacy in melanoma immunotherapy. The OT-I melanoma mouse model provides a well-established T cell mediated immune response to ovalbumin (OVA) positive B16 melanoma cells. B16/OVA melanoma cells were implanted in wild type C57Bl6 mice. Mouse splenocytes were isolated from C57Bl6 OT-I mice and were gene modified using piggyBac to express luciferase. Adoptive transfer of luciferase-modified OT-I splenocytes demonstrated homing to B16/OVA melanoma tumors in vivo. We next gene-modified OT-I cells to express mIL-12. Adoptive transfer of mIL-12-modified mouse OT-I splenocytes delayed B16/OVA melanoma tumor growth in vivo compared to control OT-I splenocytes and improved mouse survival. Our results demonstrate that the piggyBac transposon system can be used to gene modify splenocytes and mouse T cells for evaluating adoptive immunotherapy strategies in immunocompetent mouse tumor models that may more directly mimic immunotherapy applications in humans. PMID:26473608

  3. Anti-Tumor Effects after Adoptive Transfer of IL-12 Transposon-Modified Murine Splenocytes in the OT-I-Melanoma Mouse Model.

    PubMed

    Galvan, Daniel L; O'Neil, Richard T; Foster, Aaron E; Huye, Leslie; Bear, Adham; Rooney, Cliona M; Wilson, Matthew H

    2015-01-01

    Adoptive transfer of gene modified T cells provides possible immunotherapy for patients with cancers refractory to other treatments. We have previously used the non-viral piggyBac transposon system to gene modify human T cells for potential immunotherapy. However, these previous studies utilized adoptive transfer of modified human T cells to target cancer xenografts in highly immunodeficient (NOD-SCID) mice that do not recapitulate an intact immune system. Currently, only viral vectors have shown efficacy in permanently gene-modifying mouse T cells for immunotherapy applications. Therefore, we sought to determine if piggyBac could effectively gene modify mouse T cells to target cancer cells in a mouse cancer model. We first demonstrated that we could gene modify cells to express murine interleukin-12 (p35/p40 mIL-12), a transgene with proven efficacy in melanoma immunotherapy. The OT-I melanoma mouse model provides a well-established T cell mediated immune response to ovalbumin (OVA) positive B16 melanoma cells. B16/OVA melanoma cells were implanted in wild type C57Bl6 mice. Mouse splenocytes were isolated from C57Bl6 OT-I mice and were gene modified using piggyBac to express luciferase. Adoptive transfer of luciferase-modified OT-I splenocytes demonstrated homing to B16/OVA melanoma tumors in vivo. We next gene-modified OT-I cells to express mIL-12. Adoptive transfer of mIL-12-modified mouse OT-I splenocytes delayed B16/OVA melanoma tumor growth in vivo compared to control OT-I splenocytes and improved mouse survival. Our results demonstrate that the piggyBac transposon system can be used to gene modify splenocytes and mouse T cells for evaluating adoptive immunotherapy strategies in immunocompetent mouse tumor models that may more directly mimic immunotherapy applications in humans. PMID:26473608

  4. Glucuronidation of OTS167 in Humans Is Catalyzed by UDP-Glucuronosyltransferases UGT1A1, UGT1A3, UGT1A8, and UGT1A10

    PubMed Central

    Ramírez, Jacqueline; Mirkov, Snezana; House, Larry K.

    2015-01-01

    OTS167 is a potent maternal embryonic leucine zipper kinase inhibitor undergoing clinical testing as antineoplastic agent. We aimed to identify the UDP-glucuronosyltransferases (UGTs) involved in OTS167 metabolism, study the relationship between UGT genetic polymorphisms and hepatic OTS167 glucuronidation, and investigate the inhibitory potential of OTS167 on UGTs. Formation of a single OTS167-glucuronide (OTS167-G) was observed in pooled human liver (HLM) (Km = 3.4 ± 0.2 µM), intestinal microsomes (HIM) (Km = 1.7 ± 0.1 µM), and UGTs. UGT1A1 (64 µl/min/mg) and UGT1A8 (72 µl/min/mg) exhibited the highest intrinsic clearances (CLint) for OTS167, followed by UGT1A3 (51 µl/min/mg) and UGT1A10 (47 µl/min/mg); UGT1A9 was a minor contributor. OTS167 glucuronidation in HLM was highly correlated with thyroxine glucuronidation (r = 0.91, P < 0.0001), SN-38 glucuronidation (r = 0.79, P < 0.0001), and UGT1A1 mRNA (r = 0.72, P < 0.0001). Nilotinib (UGT1A1 inhibitor) and emodin (UGT1A8 and UGT1A10 inhibitor) exhibited the highest inhibitory effects on OTS167-G formation in HLM (68%) and HIM (47%). We hypothesize that OTS167-G is an N-glucuronide according to mass spectrometry. A significant association was found between rs6706232 and reduced OTS167-G formation (P = 0.03). No or weak UGT inhibition (range: 0–21%) was observed using clinically relevant OTS167 concentrations (0.4–2 µM). We conclude that UGT1A1 and UGT1A3 are the main UGTs responsible for hepatic formation of OTS167-G. Intestinal UGT1A1, UGT1A8, and UGT1A10 may contribute to first-pass OTS167 metabolism after oral administration. PMID:25870101

  5. Spectrum of M31 nova candidate M31N 2016-04a (MASTER OT J004528.12+414117.6)

    NASA Astrophysics Data System (ADS)

    Williams, S. C.; Darnley, M. J.

    2016-06-01

    We obtained a spectrum of nova candidate M31N 2016-04a (MASTER OT J004528.12+414117.6; ATel #8950) with the SPRAT spectrograph (Piascik et al. 2014) on the 2m Liverpool Telescope (Steele et al. 2004) on 2016 June 3.19 UT, 54 days after discovery.

  6. MAGIC detects very high energy gamma-ray emission from the blazar OT 081 (PKS 1749+096, 4C +09.57)

    NASA Astrophysics Data System (ADS)

    Mirzoyan, Razmik

    2016-07-01

    The MAGIC collaboration reports on the detection of very high energy (VHE; E > 150 GeV) gamma-ray emission from OT 081 (RA=17 51 32.82, dec=+09 39 00.73, J2000.0; also known as PKS 1749+096 and 4C +09.57).

  7. /sup 45/Ca efflux for myometrial cells: comparison of the effects of prostaglandin F/sub 2/. cap alpha. (PGF/sub 2/), oxytocin (OT) and arachidonate (A)

    SciTech Connect

    Katona, G.; Molnar, M.; Toth, M.; Hertelendy, F.

    1986-03-01

    The aim of this study was to measure PGF/sub 2..cap alpha../-induced Ca/sup 2 +/ release from uterine cells and to compare this to the actions of OT and A. Smooth muscle cells isolated from the uterus (shell gland) of laying hens were cultured for 7 days in M199 plus 10% fetal calf serum. The cells were treated with digitonin (20..mu..M) and preloaded with /sup 45/Ca for 40 min. Addition of PGF/sub 2..cap alpha../ caused a biphasic /sup 45/Ca-efflux. There was a small but significant /sup 45/Ca-release within 30 sec (rapid phase) followed by a larger one within 7 min (slow phase). In comparison, both OT and A stimulated /sup 45/Ca efflux during a single, slow phase. The maximal effect of A was observed at < 7 min, whereas that of OT was slower, peaking after 7 min. Mepacrin, an inhibitor of A release, attenuated the action of OT without having any effect on A promoted /sup 45/Ca-efflux. Indomethacin, an inhibitor of PG synthase, failed to suppress the Ca-releasing effect of A suggesting the A itself or a lipoxygenase product may have been responsible for the observed effects. Moreover, these results provide suggestive evidence that A release is an important step in the action of various uterotonic agents converging on the mobilization of intracellular Ca.

  8. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water? 150.445 Section 150.445 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) DEEPWATER PORTS DEEPWATER PORTS: OPERATIONS Cargo Transfer Operations §...

  9. Photometry of the MASTER OT J004207.99+405501.1 = M31N 2015-01a Bright Nova in Andromeda Galaxy

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Pruzhinskaya, M.; Tiurina, N.; Lipunov, V.; Gorbovskoy, E.; Balanutsa, P.; Denisenko, D.; Vladimirov, V.; Rufanov, A.; Kuznetsov, A.; Buckley, D.; Potter, S.; Kniazev, A.; Kotze, M.; Ivanov, K.; Gres, O.; Yazev, S.; Budnev, N.; Poleshchuk, V.; Konstantinov, E.; Tlatov, A.; Dormidontov, D.; Senik, V.; Parkhomenko, A.; Yurkov, V.; Sergienko, Y.; Varda, D.; Sinyakov, E.; Gabovich, A.; Krushinsky, V.; Zalozhnih, I.; Popov, A.; Bourdanov, A.; Shurpakov, S.

    2015-01-01

    MASTER OT J004207.99+405501.1 = M31N 2015-01a - Bright Nova in Andromeda Galaxy MASTER-Kislovodsk auto-detection system discovered PSN source at (RA, Dec) = 00h 42m 07.99s +40d 55m 01.1s on 2015-01-13.63235 UT (Shumkov et al., #6911.) .

  10. 12 CFR 516.290 - What will happen if OTS does not approve or disapprove my application within two calendar years...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false What will happen if OTS does not approve or disapprove my application within two calendar years after the filing date? 516.290 Section 516.290 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES...

  11. 12 CFR 516.290 - What will happen if OTS does not approve or disapprove my application within two calendar years...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true What will happen if OTS does not approve or disapprove my application within two calendar years after the filing date? 516.290 Section 516.290 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES...

  12. Design, synthesis and biological activity of new neurohypophyseal hormones analogues conformationally restricted in the N-terminal part of the molecule. Highly potent OT receptor antagonists.

    PubMed

    Kwiatkowska, Anna; Ptach, Monika; Borovičková, Lenka; Slaninová, Jiřina; Lammek, Bernard; Prahl, Adam

    2012-08-01

    In this study we present the synthesis and some pharmacological properties of fourteen new analogues of neurohypophyseal hormones conformationally restricted in the N-terminal part of the molecule. All new peptides were substituted at position 2 with cis-1-amino-4-phenylcyclohexane-1-carboxylic acid (cis-Apc). Moreover, one of the new analogues: [cis-Apc(2), Val(4)]AVP was also prepared in N-acylated forms with various bulky acyl groups. All the peptides were tested for pressor, antidiuretic, and in vitro uterotonic activities. We also determined the binding affinity of the selected compounds to human OT receptor. Our results showed that introduction of cis -Apc(2) in position 2 of either AVP or OT resulted in analogues with high antioxytocin potency. Two of the new compounds, [Mpa(1),cis-Apc(2)]AVP and [Mpa(1),cis-Apc(2),Val(4)]AVP, were exceptionally potent antiuterotonic agents (pA(2) = 8.46 and 8.40, respectively) and exhibited higher affinities for the human OT receptor than Atosiban (K (i) values 5.4 and 9.1 nM). Moreover, we have demonstrated for the first time that N -terminal acylation of AVP analogue can improve its selectivity. Using this approach, we obtained compound Aba[cis-Apc(2),Val(4)]AVP (XI) which turned out to be a moderately potent and exceptionally selective OT antagonist (pA(2) = 7.26). PMID:22038179

  13. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water? 150.445 Section 150.445 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) DEEPWATER PORTS DEEPWATER PORTS: OPERATIONS Cargo Transfer Operations §...

  14. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water? 150.445 Section 150.445 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) DEEPWATER PORTS DEEPWATER PORTS: OPERATIONS Cargo Transfer Operations §...

  15. AVESTAR Center for operational excellence of clean energy plants and DYNSIM OTS / EyeSim ITS integration

    SciTech Connect

    Provost, G

    2012-01-01

    This Power-Point presentation with notes starts with a brief overview of US energy challenging, particularly as regards power generation capacity and clean energy plant operations. It then goes on to present Advanced Virtual Energy Simulation Training And Research (AVESTAR{trademark}) beginning with a statement of its missions and goals, then moves to the subject of Integrated Gasification Combined Cycle (IGCC) with CO{sub 2} Capture, first providing a brief overview of the process, then moving on to Dynamic Simulator/Operator Training System (OTS) and 3D Virtual Immersive Training System (ITS). The presentation continues to describe AVESTAR center facilities, locations, and training systems and to look at future directions for virtual energy simulation.

  16. Probing the internal dynamics of reverse micelles formed in highly compressible solvents: Aerosol-OT in near-critical propane

    SciTech Connect

    Zhang, J.; Bright, F.V.

    1992-10-29

    In normal liquids, the rate of water reorganization within the interior of AOT (sodium bis(2-ethylhexyl) sulfosuccinate or Aerosol-OT) reverse micelles in a strong function of water loading and temperature. In this report, we discuss our most recent efforts to understand the internal dynamics of reverse micelles maintained in highly compressible solvents (near-critical propane). By using steady-state and time-resolved fluorescence spectroscopy, we report the first evidence for pressure-assisted control of the rate of solvent relaxation within the core region of a reverse micelle maintained in a highly compressible fluid. Three factors are taken into account in this study: amount of water loading, temperature, and bulk fluid density. The key conclusion is that the continuous phase density can influence the rate of solvent reorientation within the interior of AOT micelles formed in near-critical propane. 58 refs., 10 figs., 3 tabs.

  17. DUSTY EXPLOSIONS FROM DUSTY PROGENITORS: THE PHYSICS OF SN 2008S AND THE 2008 NGC 300-OT

    SciTech Connect

    Kochanek, C. S.

    2011-11-01

    SN 2008S and the 2008 NGC 300-OT were explosive transients of stars self-obscured by very dense, dusty stellar winds. An explosive transient with an unobserved shock breakout luminosity of order 10{sup 10} L{sub sun} is required to render the transients little obscured and visible in the optical at their peaks. Such a large breakout luminosity then implies that the progenitor stars were cool, red supergiants, most probably {approx}9 M{sub sun} extreme asymptotic giant branch stars. As the shocks generated by the explosions propagate outward through the dense wind, they produce a shock luminosity in soft X-rays that powers the long-lived luminosity of the transients. Unlike typical cases of transients exploding into a surrounding circumstellar medium, the progenitor winds in these systems are optically thick to soft X-rays, easily absorb radio emission, and rapidly reform dust destroyed by the peak luminosity of the transients. As a result, X-rays are absorbed by the gas and the energy is ultimately radiated by the reformed dust. Three years post-peak, both systems are still significantly more luminous than their progenitor stars, but they are again fully shrouded by the reformed dust and only visible in the mid-IR. The high luminosity and heavy obscuration may make it difficult to determine the survival of the progenitor stars for {approx}10 years. However, our model indicates that SN 2008S, but not the NGC 300-OT, should now be a detectable X-ray source. SN 2008S has a higher estimated shock velocity and a lower density wind, so the X-rays begin to escape at a much earlier phase.

  18. The Viscous Behavior of FeOt-Al2O3-SiO2 Copper Smelting Slags

    NASA Astrophysics Data System (ADS)

    Park, Hyun-Shik; Park, Su Sang; Sohn, Il

    2011-08-01

    Understanding the viscous behavior of copper smelting slags is essential in increasing the process efficiency and obtaining the discrete separation between the matte and the slag. The viscosity of the FeOt-SiO2-Al2O3 copper smelting slags was measured in the current study using the rotating spindle method. The viscosity at a fixed Al2O3 concentration decreased with increasing Fe/SiO2 ratio because of the depolymerization of the molten slag by the network-modifying free oxygen ions (O2-) supplied by FeO. The Fourier transform infrared (FTIR) analyses of the slag samples with increasing Fe/SiO2 ratio revealed that the amount of large silicate sheets decreased, whereas the amount of simpler silicate structures increased. Al2O3 additions to the ternary FeOt-SiO2-Al2O3 slag system at a fixed Fe/SiO2 ratio showed a characteristic V-shaped pattern, where initial additions decreased the viscosity, reached a minimum, and increased subsequently with higher Al2O3 content. The effect of Al2O3 was considered to be related to the amphoteric behavior of Al2O3, where Al2O3 initially behaves as a basic oxide and changes to an acidic oxide with variation in slag composition. Furthermore, Al2O3 additions also resulted in the high temperature phase change between fayalite/hercynite and the modification of the liquidus temperature with Al2O3 additions affecting the viscosity of the copper smelting slag.

  19. THE DIVERSITY OF MASSIVE STAR OUTBURSTS. I. OBSERVATIONS OF SN2009ip, UGC 2773 OT2009-1, AND THEIR PROGENITORS

    SciTech Connect

    Foley, Ryan J.; Berger, Edo; Challis, Peter J.; Soderberg, Alicia M.; Fox, Ori; Levesque, Emily M.; Ivans, Inese I.; Rhoads, James E.

    2011-05-01

    Despite both being outbursts of luminous blue variables (LBVs), SN 2009ip and UGC 2773 OT2009-1 have very different progenitors, spectra, circumstellar environments, and possibly physical mechanisms that generated the outbursts. From pre-eruption Hubble Space Telescope images, we determine that SN 2009ip and UGC 2773 OT2009-1 have initial masses of {approx}> 60 and {approx}> 25 M{sub sun}, respectively. Optical spectroscopy shows that at peak, SN 2009ip had a 10,000 K photosphere and its spectrum was dominated by narrow H Balmer emission, similar to classical LBV giant outbursts, also known as 'supernova impostors'. The spectra of UGC 2773 OT2009-1, which also have narrow H{alpha} emission, are dominated by a forest of absorption lines, similar to an F-type supergiant. Blueshifted absorption lines corresponding to ejecta at a velocity of 2000-7000 km s{sup -1} are present in later spectra of SN 2009ip-an unprecedented observation for LBV outbursts, indicating that the event was the result of a supersonic explosion rather than a subsonic outburst. The velocity of the absorption lines increases between two epochs, suggesting that there were two explosions in rapid succession. A rapid fading and rebrightening event concurrent with the onset of the high-velocity absorption lines is consistent with the double-explosion model. A near-infrared excess is present in the spectra and photometry of UGC 2773 OT2009-1 that is consistent with {approx}2100 K dust emission. We compare the properties of these two events and place them in the context of other known massive star outbursts such as {eta} Car, NGC 300 OT2008-1, and SN 2008S. This qualitative analysis suggests that massive star outbursts have many physical differences that can manifest as the different observables seen in these two interesting objects.

  20. Down-Regulatory Effects of miR-211 on Long Non-Coding RNA SOX2OT and SOX2 Genes in Esophageal Squamous Cell Carcinoma

    PubMed Central

    Shafiee, Mohammad; Aleyasin, Seyed Ahmad; Vasei, Mohammad; Semnani, Shahriar Semnani; Mowla, Seyed Javad

    2016-01-01

    Objective MicroRNAs (miRNAs) are a class of non-coding RNAs (ncRNAs) that tran- scriptionally or post-transcriptionally regulate gene expression through degradation of their mRNA targets and/or translational suppression. However, there are a few reports on miRNA-mediated expression regulation of long ncRNAs (lncRNAs). We have previ- ously reported a significant upregulation of the lncRNA SOX2OT and its intronic cod- ing gene, SOX2, in esophageal squamous cell carcinoma (ESCC) tissue samples. In this study, we aimed to evaluate the effect of induced overexpression of miR-211 on SOX2OT and SOX2 expression in vitro. Materials and Methods In this experimental study, we performed both bioinformatic and experimental analyses to examine whether these transcripts are regulated by miRNAs. From the list of potential candidate miRNAs, miR-211 was found to have complementary sequences to SOX2OT and SOX2 transcripts. To validate our finding experimentally, we transfected the NT-2 pluripotent cell line (an embryonal carcinoma stem cell) with an expression vector overexpressing miR-211. The expression chang- es of miR-211, SOX2OT, and SOX2 were then quantified by a real-time polymerase chain reaction (RT-PCR) approach. Results Compared with mock-transfected cells, overexpression of miR-211 caused a significant down-regulation of both genes (P<0.05). Furthermore, flow-cytometry analysis revealed a significant elevation in sub-G1 cell population following ectopic expression of miR-211 in NT-2 cells. Conclusion We report here, for the first time, the down-regulation of SOX2OT and SOX2 genes by an miRNA. Considering the vital role of SOX2OT and SOX2 genes in pluripotency and tumorigenesis, our data suggest an important and inhibitory role for miR-211 in the aforementioned processes. PMID:26862518

  1. Mutagenesis of Burkholderia pseudomallei with Tn5-OT182: isolation of motility mutants and molecular characterization of the flagellin structural gene.

    PubMed Central

    DeShazer, D; Brett, P J; Carlyon, R; Woods, D E

    1997-01-01

    Burkholderia pseudomallei is a human and animal pathogen in tropical regions, especially Southeast Asia and northern Australia. Currently little is known about the genetics and molecular biology of this organism. In this report, we describe the mutagenesis of B. pseudomallei with the transposon Tn5-OT182. B. pseudomallei 1026b transposon mutants were obtained at a frequency of 4.6 x 10(-4) per initial donor cell, and the transposon inserted randomly into the chromosome. We used Tn5-OT182 to identify the flagellin structural gene, fliC. We screened 3,500 transposon mutants and identified 28 motility mutants. Tn5-OT182 integrated into 19 unique genetic loci encoding proteins with homology to Escherichia coli and Salmonella typhimurium flagellar and chemotaxis proteins. Two mutants, MM35 and MM36, contained Tn5-OT182 integrations in fliC. We cloned and sequenced fliC and used it to complement MM35 and MM36 in trans. The fliC transcriptional start site and a sigmaF-like promoter were identified by primer extension analysis. We observed a significant difference in the expression of two distinct fliC-lacZ transcriptional fusions during bacterial growth, suggesting the presence of a latent intragenic transcriptional terminator in fliC. There was no significant difference in the virulence of 1026b compared to that of MM36 in diabetic rats or Syrian hamsters, suggesting that flagella and/or motility are probably not virulence determinants in these animal models of B. pseudomallei infection. A phylogenetic analysis based on the flagellins from a variety of bacterial species supported the recent transfer of B. pseudomallei from the genus Pseudomonas to Burkholderia. PMID:9079894

  2. Genome sequence of Ostreococcus tauri virus OtV-2 throws light on the role of picoeukaryote niche separation in the ocean.

    PubMed

    Weynberg, Karen D; Allen, Michael J; Gilg, Ilana C; Scanlan, David J; Wilson, William H

    2011-05-01

    Ostreococcus tauri, a unicellular marine green alga, is the smallest known free-living eukaryote and is ubiquitous in the surface oceans. The ecological success of this organism has been attributed to distinct low- and high-light-adapted ecotypes existing in different niches at a range of depths in the ocean. Viruses have already been characterized that infect the high-light-adapted strains. Ostreococcus tauri virus (OtV) isolate OtV-2 is a large double-stranded DNA algal virus that infects a low-light-adapted strain of O. tauri and was assigned to the algal virus family Phycodnaviridae, genus Prasinovirus. Our working hypothesis for this study was that different viruses infecting high- versus low-light-adapted O. tauri strains would provide clues to propagation strategies that would give them selective advantages within their particular light niche. Sequence analysis of the 184,409-bp linear OtV-2 genome revealed a range of core functional genes exclusive to this low-light genotype and included a variety of unexpected genes, such as those encoding an RNA polymerase sigma factor, at least four DNA methyltransferases, a cytochrome b(5), and a high-affinity phosphate transporter. It is clear that OtV-2 has acquired a range of potentially functional genes from its host, other eukaryotes, and even bacteria over evolutionary time. Such piecemeal accretion of genes is a trademark of large double-stranded DNA viruses that has allowed them to adapt their propagation strategies to keep up with host niche separation in the sunlit layers of the oceanic environment. PMID:21289127

  3. Adsorption of Aerosol-OT at the calcite/water interface--comparison of the sodium and calcium salts.

    PubMed

    Stocker, Isabella N; Miller, Kathryn L; Welbourn, Rebecca J L; Clarke, Stuart M; Collins, Ian R; Kinane, Christian; Gutfreund, Philipp

    2014-03-15

    The adsorption of the surfactant Aerosol-OT (AOT) at the calcite-water interface has been investigated using batch adsorption isotherms and neutron reflection. The adsorption isotherms showed that NaAOT adsorption followed S-type adsorption behaviour with a maximum surface excess of 2.5 mg m(-2) but the method could not be used for the investigation of Ca(AOT)2 adsorption owing to the changes in the bulk phase behaviour of the solution. The surface excess, determined by neutron reflection at the critical micelle concentration (CMC), was 2.5 mg m(-2) for Ca(AOT)2 and 1.8 mg m(-2) for NaAOT. The time dependence of the NaAOT adsorption suggests a slow conversion from the sodium to the calcium salt of AOT at the calcite-water interface by binding calcium ions released from the slightly soluble calcite. The layer thickness in both cases was 35 Å which indicates adsorption as bilayers or distorted micelles. At higher concentrations of NaAOT (~10× CMC) adsorption of an AOT lamellar phase was evident from Bragg peaks in the specular reflection. To our knowledge, this is the first time that adsorption of a surfactant at the calcite-water interface has been investigated by neutron reflection. The technique provided significant new insight into the adsorption behaviour of AOT which would not have been accessible using traditional techniques. PMID:24461829

  4. Overexpression, purification, crystallization and preliminary crystallographic studies of a hyperthermophilic adenylosuccinate synthetase from Pyrococcus horikoshii OT3

    PubMed Central

    Wang, Xiaoying; Akasaka, Ryogo; Takemoto, Chie; Morita, Satoshi; Yamaguchi, Machiko; Terada, Takaho; Shirozu, Mikako; Yokoyama, Shigeyuki; Chen, Shilin; Si, Shuyi; Xie, Yong

    2011-01-01

    Adenylosuccinate synthetase (AdSS) is a ubiquitous enzyme that catalyzes the first committed step in the conversion of inosine monophosphate (IMP) to adenosine monophosphate (AMP) in the purine-biosynthetic pathway. Although AdSS from the vast majority of organisms is 430–457 amino acids in length, AdSS sequences isolated from thermophilic archaea are 90–120 amino acids shorter. In this study, crystallographic studies of a short AdSS sequence from Pyrococcus horikoshii OT3 (PhAdSS) were performed in order to reveal the unusual structure of AdSS from thermophilic archaea. Crystals of PhAdSS were obtained by the microbatch-under-oil method and X-ray diffraction data were collected to 2.50 Å resolution. The crystal belonged to the trigonal space group P3212, with unit-cell parameters a = b = 57.2, c = 107.9 Å. There was one molecule per asymmetric unit, giving a Matthews coefficient of 2.17 Å3 Da−1 and an approximate solvent content of 43%. In contrast, the results of native polyacrylamide gel electrophoresis and analytical ultracentrifugation showed that the recombinant PhAdSS formed a dimer in solution. PMID:22139164

  5. Purification, crystallization and preliminary crystallographic analysis of archaeal 6-pyruvoyl tetrahydrobiopterin synthase homologue PH0634 from Pyrococcus horikoshii OT3.

    PubMed

    Bagautdinov, Bagautdin; Sugahara, Mitsuaki; Kunishima, Naoki

    2007-01-01

    6-Pyruvoyl tetrahydrobiopterin synthase (PTPS) catalyses the conversion of dihydroneopterin triphosphate to 6-pyruvoyl tetrahydropterin, the second of the three enzymatic steps in the synthesis of tetrahydrobiopterin from GTP. PH0634, a 13.51 kDa archaeal PTPS homologue from Pyrococcus horikoshii OT3, was overexpressed as native and selenomethionine-substituted protein and the purified protein was crystallized by the oil-microbatch method at 295 K. X-ray diffraction data were collected to 2.1 A resolution from the native crystal using synchrotron radiation at 100 K. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 35.83, b = 95.71, c = 105.65 A. Threefold noncrystallographic symmetry was identified from self-rotation calculations. Assuming the presence of a trimer in the asymmetric unit, the solvent content is 45% (V(M) = 2.24 A3 Da(-1)). The selenomethionine-substituted crystal is isomorphous to the native crystal and diffracts X-rays to 2.9 A. PMID:17183164

  6. Purification, crystallization and preliminary crystallographic analysis of RecA superfamily ATPase PH0284 from Pyrococcus horikoshii OT3.

    PubMed

    Bagautdinov, Bagautdin; Kunishima, Naoki

    2006-04-01

    Circadian (daily) protein clocks are found in cyanobacteria, where a complex of the KaiA, KaiB and KaiC proteins generates circadian rhythms. The 28.09 kDa KaiC homologue PH0284 protein from Pyrococcus horikoshii OT3 was cloned and expressed and the purified protein was crystallized by the oil-microbatch method at 295 K. X-ray diffraction data from the crystal were collected to 2.0 angstroms resolution using synchrotron radiation at 100 K. The crystal belongs to the trigonal space group P3(2)21, with unit-cell parameters a = b = 96.06, c = 298.90 angstroms. Assuming the presence of one hexamer in the asymmetric unit gives a V(M) value of 2.36 angstroms3 Da(-1) and a solvent content of 47.9%. A cocrystal with ATP was prepared and a diffraction data set was collected at 2.3 angstroms resolution. PMID:16582499

  7. Purification, crystallization and preliminary crystallographic analysis of archaeal 6-pyruvoyl tetrahydrobiopterin synthase homologue PH0634 from Pyrococcus horikoshii OT3

    PubMed Central

    Bagautdinov, Bagautdin; Sugahara, Mitsuaki; Kunishima, Naoki

    2007-01-01

    6-Pyruvoyl tetrahydrobiopterin synthase (PTPS) catalyses the conversion of dihydroneopterin triphosphate to 6-pyruvoyl tetrahydropterin, the second of the three enzymatic steps in the synthesis of tetrahydrobiopterin from GTP. PH0634, a 13.51 kDa archaeal PTPS homologue from Pyrococcus horikoshii OT3, was overexpressed as native and selenomethionine-substituted protein and the purified protein was crystallized by the oil-microbatch method at 295 K. X-ray diffraction data were collected to 2.1 Å resolution from the native crystal using synchrotron radiation at 100 K. The crystal belongs to the orthorhombic space group P212121, with unit-cell parameters a = 35.83, b = 95.71, c = 105.65 Å. Threefold noncrystallographic symmetry was identified from self-rotation calculations. Assuming the presence of a trimer in the asymmetric unit, the solvent content is 45% (V M = 2.24 Å3 Da−1). The selenomethionine-substituted crystal is isomorphous to the native crystal and diffracts X-rays to 2.9 Å. PMID:17183164

  8. Purification, crystallization and preliminary crystallographic analysis of the biotin-protein ligase from Pyrococcus horikoshii OT3.

    PubMed

    Bagautdinov, Bagautdin; Kuroishi, Chizu; Sugahara, Mitsuaki; Kunishima, Naoki

    2005-02-01

    Biotin-protein ligase is an enzyme that catalyzes the ATP-dependent biotinylation of a specific lysine residue in acetyl-CoA carboxylase. The biotin-protein ligase from Pyrococcus horikoshii OT3 has been cloned, overexpressed and purified. Crystallization was performed by the microbatch method or the vapour-diffusion method using PEG 2000 as a precipitant at 295 K. X-ray diffraction data have been collected to 1.6 A resolution from a native crystal and to 1.55 A resolution from a selenomethionine-derivative crystal for multiple anomalous dispersion phasing using synchrotron radiation at 100 K. The native crystal belongs to the monoclinic space group P2(1), with unit-cell parameters a = 38.601, b = 78.264, c = 70.147 A, beta = 101.48 degrees. Assuming a homodimer per asymmetric unit gives a VM value of 2.14 A3 Da(-1) and a solvent content of 42.5%. Cocrystals with biotin, ADP and biotinyl-5'-AMP were prepared and diffraction data sets were collected to 1.6, 1.6 and 1.45 A resolution, respectively. PMID:16510991

  9. Purification, crystallization and preliminary crystallographic analysis of the biotin–protein ligase from Pyrococcus horikoshii OT3

    PubMed Central

    Bagautdinov, Bagautdin; Kuroishi, Chizu; Sugahara, Mitsuaki; Kunishima, Naoki

    2005-01-01

    Biotin–protein ligase is an enzyme that catalyzes the ATP-dependent biotinylation of a specific lysine residue in acetyl-CoA carboxylase. The biotin–protein ligase from Pyrococcus horikoshii OT3 has been cloned, overexpressed and purified. Crystallization was performed by the microbatch method or the vapour-diffusion method using PEG 2000 as a precipitant at 295 K. X-ray diffraction data have been collected to 1.6 Å resolution from a native crystal and to 1.55 Å resolution from a selenomethionine-derivative crystal for multiple anomalous dispersion phasing using synchrotron radiation at 100 K. The native crystal belongs to the monoclinic space group P21, with unit-cell parameters a = 38.601, b = 78.264, c  =  70.147 Å, β = 101.48°. Assuming a homodimer per asymmetric unit gives a V M value of 2.14 Å3 Da−1 and a solvent content of 42.5%. Cocrystals with biotin, ADP and biotinyl-5′-AMP were prepared and diffraction data sets were collected to 1.6, 1.6 and 1.45 Å resolution, respectively. PMID:16510991

  10. Purification, crystallization and preliminary crystallographic analysis of RecA superfamily ATPase PH0284 from Pyrococcus horikoshii OT3

    PubMed Central

    Bagautdinov, Bagautdin; Kunishima, Naoki

    2006-01-01

    Circadian (daily) protein clocks are found in cyanobacteria, where a complex of the KaiA, KaiB and KaiC proteins generates circadian rhythms. The 28.09 kDa KaiC homologue PH0284 protein from Pyrococcus horikoshii OT3 was cloned and expressed and the purified protein was crystallized by the oil-microbatch method at 295 K. X-ray diffraction data from the crystal were collected to 2.0 Å resolution using synchrotron radiation at 100 K. The crystal belongs to the trigonal space group P3221, with unit-cell parameters a = b = 96.06, c = 298.90 Å. Assuming the presence of one hexamer in the asymmetric unit gives a V M value of 2.36 Å3 Da−1 and a solvent content of 47.9%. A cocrystal with ATP was prepared and a diffraction data set was collected at 2.3 Å resolution. PMID:16582499

  11. Perovskite LaFeO3 nanoparticles synthesized by the reverse microemulsion nanoreactors in the presence of aerosol-OT: Morphology, crystal structure, and their optical properties

    NASA Astrophysics Data System (ADS)

    Abazari, Reza; Sanati, Soheila

    2013-12-01

    Orthorhombic structure of lanthanum ferrite nanoparticles (LaFeO3 NPs) with perovskite type phase has been synthesized with water-in-oil (W/O) microemulsion consisted of water/dioctyl sulfosuccinate sodium (aerosol-OT)/isooctane at room temperature. It has been shown that aerosol-OT reverse microemulsion solution is appropriate for synthesizing perovskite LaFeO3 NPs in the absence of any co-surfactants. Field emission scanning electron microscope (FE-SEM), transmission electron microscopy (TEM), energy dispersive analysis of X-ray (EDAX), X-ray diffraction (XRD), ultraviolet-visible absorption spectroscopy (UV-Vis), and Fourier transform infrared spectroscopy (FT-IR) have been adopted for characterization of surface morphology, size, phase composition, structure, and optical properties of the considered NPs. Furthermore, the optical properties of LaFeO3 NPs have been further analyzed via photoluminescence (PL) spectroscopy. As shown by the physicochemical characterizations, our prepared NPs via aerosol-OT reverse microemulsion solution are spherical and nearly uniform (with a size of about 24.65 nm). Besides, they include an orthorhombic phase while no impurities are observed. Single phase lanthanum ferrite NPs have successfully been prepared at 500 °C. Moreover, UV-Vis spectrum indicates that the LaFeO3 NPs synthesized through this technique can be considered as a type of photo-catalytic materials.

  12. Simultaneous Photometry and Spectroscopy of the Deeply Absorbing Polar MASTER OT J132104.04+560957.8

    NASA Astrophysics Data System (ADS)

    Hoyt, Taylor; Littlefield, Colin; Garnavich, Peter M.

    2016-01-01

    We present photometry and time-resolved spectroscopy of the suspected polar MASTER OT J132104.04+560957.8 (J1321) taken at the Large Binocular Telescope (LBT) simultaneously with the LBC camera and MODS spectrograph in binocular mode. Our V-band photometry confirmed a primary and secondary maximum first identified by Littlefield et al. In the spectra we see unusually deep Balmer and helium absorption features for about 10-15% of a 91 minute orbital period. The absorption lines first appear to the red of the emission peaks then shift bluewards, ultimately dominating the line profile at zero radial velocity. Towards the end of the absorption phase, weak absorption reappears to the blue of the emission lines. When the system is bright the spectra show a broadly-peaked continuum with a maximum around 500nm. We do not see absorption during the secondary bright red phase. Around the minima the continuum is blue and resembles a more typical polar spectrum with strong Balmer, neutral helium and ionized helium emission lines. The Doppler tomograms of J1321 are fuzzy and blobby and resemble those of the asynchronous polar BY Cam. The highly variable width of the secondary maximum remains a mystery along with the lack of a discernible irradiated secondary in the tomograms. We use our observations to constrain the properties of the white dwarf's magnetic field as well as the accretion flow structure. We also discuss evidence of J1321 being in a prolonged low-mass-transfer state in all observations prior to 2007.

  13. ADP-dependent 6-Phosphofructokinase from Pyrococcus horikoshii OT3: STRUCTURE DETERMINATION AND BIOCHEMICAL CHARACTERIZATION OF PH1645

    SciTech Connect

    Currie, Mark A.; Merino, Felipe; Skarina, Tatiana; Wong, Andrew H.Y.; Singer, Alexander; Brown, Greg; Savchenko, Alexei; Caniuguir, Andrés; Guixé, Victoria; Yakunin, Alexander F.; Jia, Zongchao

    2009-12-01

    Some hyperthermophilic archaea use a modified glycolytic pathway that employs an ADP-dependent glucokinase (ADP-GK) and an ADP-dependent phosphofructokinase (ADP-PFK) or, in the case of Methanococcus jannaschii, a bifunctional ADP-dependent glucophosphofructokinase (ADP-GK/PFK). The crystal structures of three ADP-GKs have been determined. However, there is no structural information available for ADP-PFKs or the ADP-GK/PFK. Here, we present the first crystal structure of an ADP-PFK from Pyrococcus horikoshii OT3 (PhPFK) in both apo- and AMP-bound forms determined to 2.0-{angstrom} and 1.9-{angstrom} resolution, respectively, along with biochemical characterization of the enzyme. The overall structure of PhPFK maintains a similar large and small {alpha}/{beta} domain structure seen in the ADP-GK structures. A large conformational change accompanies binding of phosphoryl donor, acceptor, or both, in all members of the ribokinase superfamily characterized thus far, which is believed to be critical to enzyme function. Surprisingly, no such conformational change was observed in the AMP-bound PhPFK structure compared with the apo structure. Through comprehensive site-directed mutagenesis of the substrate binding pocket we identified residues that were critical for both substrate recognition and the phosphotransfer reaction. The catalytic residues and many of the substrate binding residues are conserved between PhPFK and ADP-GKs; however, four key residues differ in the sugar-binding pocket, which we have shown determine the sugar-binding specificity. Using these results we were able to engineer a mutant PhPFK that mimics the ADP-GK/PFK and is able to phosphorylate both fructose 6-phosphate and glucose.

  14. Crystal structures of biotin protein ligase from Pyrococcus horikoshii OT3 and its complexes: structural basis of biotin activation.

    PubMed

    Bagautdinov, Bagautdin; Kuroishi, Chizu; Sugahara, Mitsuaki; Kunishima, Naoki

    2005-10-21

    Biotin protein ligase (EC 6.3.4.15) catalyses the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. The three-dimensional structure of biotin protein ligase from Pyrococcus horikoshii OT3 has been determined by X-ray diffraction at 1.6A resolution. The structure reveals a homodimer as the functional unit. Each subunit contains two domains, a larger N-terminal catalytic domain and a smaller C-terminal domain. The structural feature of the active site has been studied by determination of the crystal structures of complexes of the enzyme with biotin, ADP and the reaction intermediate biotinyl-5'-AMP at atomic resolution. This is the first report of the liganded structures of biotin protein ligase with nucleotide and biotinyl-5'-AMP. The structures of the unliganded and the liganded forms are isomorphous except for an ordering of the active site loop upon ligand binding. Catalytic binding sites are suitably arranged to minimize the conformational changes required during the reaction, as the pockets for biotin and nucleotide are located spatially adjacent to each other in a cleft of the catalytic domain and the pocket for biotinyl-5'-AMP binding mimics the combination of those of the substrates. The exact locations of the ligands and the active site residues allow us to propose a general scheme for the first step of the reaction carried out by biotin protein ligase in which the positively charged epsilon-amino group of Lys111 facilitates the nucleophilic attack on the ATP alpha-phosphate group by the biotin carboxyl oxygen atom and stabilizes the negatively charged intermediates. PMID:16169557

  15. Growth of a radiation-transformed clone of C3H 1OT1/2 cells into melanin-producing colonies

    SciTech Connect

    Szekely, J.G.; Raaphorst, G.P.; Lobreau, A.U.; Azzam, E.I.; Vadasz, J.A.

    1985-01-01

    When R25, a radiation-transformed clone of C3H 1OT1/2 cells, was plated in soft agarose, a fraction of the colonies became pigmented. The morphologies of the white and dark colonies and their cells were compared by optical, transmission and scanning electron microscopy. The transformed R25 cells had apparently differentiated into melanin-producing cells in the soft agarose, with the white colonies containing actively growing cells having only a few melanosomes, and the dark colonies being made up of stationary-phase cells filled with electronopaque melanosomes. Exposure of the R25 cells to 4.0 Gy of X-rays decreased the percentage of dark colonies, while exposure to 1% DMSO had no effect.

  16. OT2_bweiner_2: Physical conditions in disky U/LIRGs from far-IR line flux ratios - low-z analogs for high-z starforming galaxies

    NASA Astrophysics Data System (ADS)

    Weiner, B.

    2011-09-01

    We propose for PACS spectroscopy of the [O III] 88 micron and [N II] 122 micron lines, and SPIRE far-IR photometry, to observe a sample of 16 low-redshift IR-luminous galaxies, at log L_IR = 11.6 to 12.2 Lsolar, that are distinguished by large size and non-merger structure. In OT1 we are obtaining PACS spectra of [C II] 158 and [O I] 63 microns for this sample. These galaxies are interesting because they have high SFR activity spread over a large physical area, rather than concentrated into extremely dense nuclear regions, as in most local major merger ULIRGs. They are good analogs for high-redshift IR-luminous galaxies, which have far-IR spectral shapes different from local ULIRGs. At z>1, much of the star formation in massive galaxies is at LIRG and ULIRG levels, and U/LIRGs dominate the IR luminosity density. Understanding star forming regions in high-z IR-luminous galaxies is necessary to understand the conditions in which most of the stars in massive galaxies formed. In a few high-z lensed ULIRGs where [C II] can be observed, [C II]/FIR is high, like local star-forming galaxies and unlike local ULIRGs. [C II] is a major cooling line in PDRs and the far-IR lines probe the physical conditions and UV intensity in IR-emitting regions. In our sample observed so far in OT1, [C II]/FIR is high - they do not suffer the "[C II] deficit" found in local ULIRGs. This suggests that redshift evolution in IR SEDs and line ratios are related to the larger extent of star formation, and that this low-z disky sample are good analogs. The [O III] and [N II] lines provide more detailed probes of the ionization state, gas density, and ionizing SEDs in the star-forming regions: in published samples the [O III]/FIR ratio shows tension with simple models for the line deficit, and [N II] and [O III] can constrain ionizing SED versus gas density. The SPIRE far-IR photometry will constrain the total IR luminosity and cold dust in these galaxies, which have cool IR colors and far

  17. Evaluation of the efficiency of tumor and tissue delivery of carrier-mediated agents (CMA) and small molecule (SM) agents in mice using a novel pharmacokinetic (PK) metric: relative distribution index over time (RDI-OT)

    PubMed Central

    Madden, Andrew J.; Rawal, Sumit; Sandison, Katie; Schell, Ryan; Schorzman, Allison; Deal, Allison; Feng, Lan; Ma, Ping; Mumper, Russell; DeSimone, Joseph; Zamboni, William C.

    2015-01-01

    The pharmacokinetics (PK) of carrier-mediated agents (CMA) is dependent upon the carrier system. As a result, CMA PK differs greatly from the PK of small molecule (SM) drugs. Advantages of CMAs over SMs include prolonged circulation time in plasma, increased delivery to tumors, increased antitumor response, and decreased toxicity. In theory, CMAs provide greater tumor drug delivery than SMs due to their prolonged plasma circulation time. We sought to create a novel PK metric to evaluate the efficiency of tumor and tissue delivery of CMAs and SMs. We conducted a study evaluating the plasma, tumor, liver, and spleen PK of CMAs and SMs in mice bearing subcutaneous flank tumors using standard PK parameters and a novel PK metric entitled relative distribution over time (RDI-OT), which measures efficiency of delivery. RDI-OT is defined as the ratio of tissue drug concentration to plasma drug concentration at each time point. The standard concentration versus time area under the curve values (AUC) of CMAs were higher in all tissues and plasma compared with SMs. However, 8 of 17 SMs had greater tumor RDI-OT AUC0–last values than their CMA comparators and all SMs had greater tumor RDI-OT AUC0–6 h values than their CMA comparators. Our results indicate that in mice bearing flank tumor xenografts, SMs distribute into tumor more efficiently than CMAs. Further research in additional tumor models that may more closely resemble tumors seen in patients is needed to determine if our results are consistent in different model systems. PMID:26392803

  18. EPA/ORD/RTP (Environmental Protection Agency, Office of Research and Development, Research Triangle Park) asbestos program support for the OTS (Office of Toxic Substances) asbestos-in-schools program. Final report

    SciTech Connect

    Beard, M.E.

    1986-03-01

    An overview of the EPA/ORD/RTP Asbestos program support for the OTS Asbestos-in-Schools Program is given. The program includes (1) a bulk sample asbestos analysis audit program, (2) an intercomparison of three measurement methods for airborne asbestos, (3) the development of audit materials for airborne asbestos measurement methods, and (4) the development of guidance for post-abatement asbestos air monitoring.

  19. Discovery of an unusual bright eclipsing binary with the longest known period: TYC 2505-672-1/MASTER OT J095310.04+335352.8

    NASA Astrophysics Data System (ADS)

    Lipunov, V.; Gorbovskoy, E.; Afanasiev, V.; Tatarnikova, A.; Denisenko, D.; Makarov, D.; Tiurina, N.; Krushinsky, V.; Vinokurov, A.; Balanutsa, P.; Kuznetsov, A.; Gress, O.; Sergienko, Yu.; Yurkov, V.; Gabovich, A.; Tlatov, A.; Senik, V.; Vladimirov, V.; Popova, E.

    2016-04-01

    We report on the MASTER Global Robotic Net discovery of an eclipsing binary, MASTER OT J095310.04+335352.8, previously known as unremarkable star TYC 2505-672-1, which displays extreme orbital parameters. The orbital period P = 69.1 yr is more than 2.5 times longer than that of ɛ-Aurigae, which is the previous record holder. The light curve is characterized by an extremely deep total eclipse with a depth of more than 4.5 mag, which is symmetrically shaped and has a total duration of 3.5 yr. The eclipse is essentially gray. The spectra acquired with the Russian 6 m BTA telescope both at minimum and maximum light mainly correspond to an M0-1III-type red giant, but the spectra taken at the bottom of eclipse show small traces of a sufficiently hot source. The observed properties of this system can be better explained as the red giant eclipsed by a large cloud (the disk) of small particles surrounding the invisible secondary companion.

  20. Crystallization and preliminary X-ray crystallographic studies of the biotin carboxyl carrier protein and biotin protein ligase complex from Pyrococcus horikoshii OT3.

    PubMed

    Bagautdinov, Bagautdin; Matsuura, Yoshinori; Bagautdinova, Svetlana; Kunishima, Naoki

    2007-04-01

    Biotin protein ligase (BPL) catalyses the biotinylation of the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase. To elucidate the exact details of the protein-protein interactions in the biotinylation function, the C-terminal half fragment of BCCP (BCCPDeltaN76), the R48A mutant of BPL (BPL*) and the R48A K111A double mutant of BPL (BPL**), all of which are from Pyrococcus horikoshii OT3, have been expressed, purified and successfully cocrystallized. Cocrystals of the BPL*-BCCPDeltaN76 and BPL**-BCCPDeltaN76 complexes as well as crystals of BPL*, BPL** and BCCPDeltaN76 were obtained by the oil-microbatch method using PEG 20 000 as a precipitant at 295 K. Complete X-ray diffraction data sets for BPL*-BCCPDeltaN76 and BPL**-BCCPDeltaN76 crystals were collected at 100 K to 2.7 and 2.0 A resolution, respectively, using synchrotron radiation. They belong to the monoclinic space group P2(1), with similar unit-cell parameters a = 69.85, b = 63.12, c = 75.64 A, beta = 95.9 degrees . Assuming two subunits of the complex per asymmetric unit gives a V(M) value of 2.45 A(3) Da(-1) and a solvent content of 50%. PMID:17401210

  1. Crystallization and preliminary X-ray crystallographic studies of the biotin carboxyl carrier protein and biotin protein ligase complex from Pyrococcus horikoshii OT3

    PubMed Central

    Bagautdinov, Bagautdin; Matsuura, Yoshinori; Bagautdinova, Svetlana; Kunishima, Naoki

    2007-01-01

    Biotin protein ligase (BPL) catalyses the biotinylation of the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase. To elucidate the exact details of the protein–protein interactions in the biotinylation function, the C-terminal half fragment of BCCP (BCCPΔN76), the R48A mutant of BPL (BPL*) and the R48A K111A double mutant of BPL (BPL**), all of which are from Pyrococcus horikoshii OT3, have been expressed, purified and successfully cocrystallized. Cocrystals of the BPL*–BCCPΔN76 and BPL**–BCCPΔN76 complexes as well as crystals of BPL*, BPL** and BCCPΔN76 were obtained by the oil-microbatch method using PEG 20 000 as a precipitant at 295 K. Complete X-ray diffraction data sets for BPL*–BCCPΔN76 and BPL**–BCCPΔN76 crystals were collected at 100 K to 2.7 and 2.0 Å resolution, respectively, using synchrotron radiation. They belong to the monoclinic space group P21, with similar unit-cell parameters a = 69.85, b = 63.12, c = 75.64 Å, β = 95.9°. Assuming two subunits of the complex per asymmetric unit gives a V M value of 2.45 Å3 Da−1 and a solvent content of 50%. PMID:17401210

  2. Identification and characterization of a thermostable bifunctional enzyme with phosphomannose isomerase and sugar-1-phosphate nucleotidylyltransferase activities from a hyperthermophilic archaeon, Pyrococcus horikoshii OT3.

    PubMed

    Akutsu, Jun-ichi; Zhang, Zilian; Morita, Rihito; Kawarabayasi, Yutaka

    2015-11-01

    Mannosylglycerate is known as a compatible solute, and plays important roles for salinity adaptation and high temperature stability of microorganisms. In the gene cluster for the mannosylglycerate biosynthetic pathway predicted from the genomic data of Pyrococcus horikoshii OT3, the PH0925 protein was found as a putative bifunctional enzyme with phosphomannose isomerase (PMI) and mannose-1-phosphate guanylyltransferase (Man-1-P GTase) activities, which can synthesize GDP-mannose when accompanied by a phosphomannomutase/phosphoglucomutase (PMM/PGM) enzyme (PH0923). The recombinant PH0925 protein, expressed in E. coli, exhibited both expected PMI and Man-1-P GTase activities, as well as absolute thermostability; 95 °C was the optimum reaction temperature. According to the guanylyltransferase activity (GTase) of the PH0925 protein, it was found that the protein can catalyze glucose-1-phosphate (Glc-1-P) and glucosamine-1-phosphate (GlcN-1-P) in addition to Man-1-P. The analyses of C-terminus-truncated forms of the PH0925 protein indicated that sugar-1-phosphate nucleotidylyltransferase (Sugar-1-P NTase) activity was located in the region from the N-terminus to the 345th residue, and that the C-terminal 114 residue region of the PH0925 protein inhibited the Man-1-P GTase activity. Conversely, the PMI activity was abolished by deletion of the C-terminal 14 residues. This is the first report of a thermostable enzyme with both PMI and multiple Sugar-1-P NTase activities. PMID:26290359

  3. OT2_sserje01_2: THE HERSCHEL-AKARI NEP DEEP SURVEY: the cosmological history of stellar mass assembly and black hole accretion

    NASA Astrophysics Data System (ADS)

    Serjeant, S.

    2011-09-01

    We propose a far-IR and submm mapping survey of the premier AKARI deep field in the North Ecliptic Pole, in PACS/SPIRE parallel mode. This is the only major deep infrared field not yet covered by Herschel guaranteed or open time key projects. The outstanding and unparalleled continuous mid-IR photometric coverage from AKARI, far better than equivalent Spitzer surveys, enables a wide range of galaxy evolution diagnostics unachievable in any other survey field (including Herschel HerMES/PEP fields), by spanning the wavelengths of redshifted PAH and silicate features and the peak energy output of AGN dust tori. The investment by AKARI in the NEP represents ~10 percent of the entire pointed observations available throughout the lifetime of AKARI. Our proposal remedies the remarkable omission from Herschel's legacy surveys of the premier extragalactic deep field from another IR space telescope. We will simultaneously identify and find photometric redshifts for the Herschel point source population, make stacking analysis detections of the galaxies which dominate the submm extragalactic background light as a function of redshift, determine the bolometric power outputs of the galaxies that dominate the submm background, compare the UV/optical/mid-IR continuum/PAH/far-IR/submm/radio star formation rate estimator in the most comprehensive IR survey data set to date, and track the coupled stellar mass assembly and black hole accretion throughout most of the history of the Universe. In OT1 the HOTAC concluded "The science output from the proposed survey will be outstanding [...] The panel was convinced that these observations should be done" but it since became clear that priority 2 time is very unlikely to be executed, so we request reclassification to priority 1.

  4. Ries Bunte Breccia revisited: Indications for the presence of water in Itzing and Otting drill cores and implications for the emplacement process

    NASA Astrophysics Data System (ADS)

    Pietrek, Alexa; Kenkmann, Thomas

    2016-04-01

    We reassessed two drill cores of the Bunte Breccia deposits of the Ries crater, Germany. The objectives of our study were the documentation of evidence for water in the Bunte Breccia, the evaluation of how that water influenced the emplacement processes, and from which preimpact water reservoir it was derived. The Bunte Breccia in both cores can be structured into a basal layer composed mainly of local substrate material, overlain by texturally and compositionally diverse, crater-derived breccia units. The basal layer is composed of the youngest sediments (Tertiary clays and Upper Jurassic limestone) and has a razor-sharp boundary to the upper breccia units, which are composed of older rocks of Upper Jurassic to Upper Triassic age. Sparse material exchange occurred between the basal layer and the rest of the Bunte Breccia. Fluids predominantly came from the Tertiary and the Upper Triassic sandstone formation. In the basal layer, Tertiary clays were subjected to intense, ductile deformation, indicating saturation with water. This suggests that water was mixed into the matrix, creating a fluidized basal layer with a strong shear localization. In the upper units, Upper Triassic sandstones are intensely deformed by granular flow. The texture requires that the rocks were disaggregated into granular sand. Vaporization of pore water probably aided fragmentation of these rocks. In the Otting core, hot suevite (T > 600 °C) covered the Bunte Breccia shortly after its emplacement. Vertically oriented gas escape pipes in suevite partly emanate directly at the contact to the Bunte Breccia. They indicate that the Bunte Breccia contained a substantial amount of water in the upper part that was vaporized and escaped through these vents.

  5. Ries Bunte Breccia revisited: Indications for the presence of water in Itzing and Otting drill cores and implications for the emplacement process

    NASA Astrophysics Data System (ADS)

    Pietrek, Alexa; Kenkmann, Thomas

    2016-07-01

    We reassessed two drill cores of the Bunte Breccia deposits of the Ries crater, Germany. The objectives of our study were the documentation of evidence for water in the Bunte Breccia, the evaluation of how that water influenced the emplacement processes, and from which preimpact water reservoir it was derived. The Bunte Breccia in both cores can be structured into a basal layer composed mainly of local substrate material, overlain by texturally and compositionally diverse, crater-derived breccia units. The basal layer is composed of the youngest sediments (Tertiary clays and Upper Jurassic limestone) and has a razor-sharp boundary to the upper breccia units, which are composed of older rocks of Upper Jurassic to Upper Triassic age. Sparse material exchange occurred between the basal layer and the rest of the Bunte Breccia. Fluids predominantly came from the Tertiary and the Upper Triassic sandstone formation. In the basal layer, Tertiary clays were subjected to intense, ductile deformation, indicating saturation with water. This suggests that water was mixed into the matrix, creating a fluidized basal layer with a strong shear localization. In the upper units, Upper Triassic sandstones are intensely deformed by granular flow. The texture requires that the rocks were disaggregated into granular sand. Vaporization of pore water probably aided fragmentation of these rocks. In the Otting core, hot suevite (T > 600 °C) covered the Bunte Breccia shortly after its emplacement. Vertically oriented gas escape pipes in suevite partly emanate directly at the contact to the Bunte Breccia. They indicate that the Bunte Breccia contained a substantial amount of water in the upper part that was vaporized and escaped through these vents.

  6. The KCNQ1OT1 imprinting control region and non-coding RNA: new properties derived from the study of Beckwith–Wiedemann syndrome and Silver–Russell syndrome cases

    PubMed Central

    Chiesa, Nicoletta; De Crescenzo, Agostina; Mishra, Kankadeb; Perone, Lucia; Carella, Massimo; Palumbo, Orazio; Mussa, Alessandro; Sparago, Angela; Cerrato, Flavia; Russo, Silvia; Lapi, Elisabetta; Cubellis, Maria Vittoria; Kanduri, Chandrasekhar; Cirillo Silengo, Margherita; Riccio, Andrea; Ferrero, Giovanni Battista

    2012-01-01

    A cluster of imprinted genes at chromosome 11p15.5 is associated with the growth disorders, Silver–Russell syndrome (SRS) and Beckwith–Wiedemann syndrome (BWS). The cluster is divided into two domains with independent imprinting control regions (ICRs). We describe two maternal 11p15.5 microduplications with contrasting phenotypes. The first is an inverted and in cis duplication of the entire 11p15.5 cluster associated with the maintenance of genomic imprinting and with the SRS phenotype. The second is a 160 kb duplication also inverted and in cis, but resulting in the imprinting alteration of the centromeric domain. It includes the centromeric ICR (ICR2) and the most 5′ 20 kb of the non-coding KCNQ1OT1 gene. Its maternal transmission is associated with ICR2 hypomethylation and the BWS phenotype. By excluding epigenetic mosaicism, cell clones analysis indicated that the two closely located ICR2 sequences resulting from the 160 kb duplication carried discordant DNA methylation on the maternal chromosome and supported the hypothesis that the ICR2 sequence is not sufficient for establishing imprinted methylation and some other property, possibly orientation-dependent, is needed. Furthermore, the 1.2 Mb duplication demonstrated that all features are present for correct imprinting at ICR2 when this is duplicated and inverted within the entire cluster. In the individuals maternally inheriting the 160 kb duplication, ICR2 hypomethylation led to the expression of a truncated KCNQ1OT1 transcript and to down-regulation of CDKN1C. We demonstrated by chromatin RNA immunopurification that the KCNQ1OT1 RNA interacts with chromatin through its most 5′ 20 kb sequence, providing a mechanism likely mediating the silencing activity of this long non-coding RNA. PMID:21920939

  7. Aerodynamic results of an abort separation effects test (IA8) conducted in the NASA/ARC 14-foot transonic wind tunnel on a model (6-OTS) of the Rockwell International launch configuration integrated vehicle

    NASA Technical Reports Server (NTRS)

    Campbell, J. H.

    1974-01-01

    Experimental aerodynamic investigations were conducted on a 6-OTS 0.015-scale model. The Ames dual sting support separation rig was used to obtain grid-type data for tank-booster abort from orbiter (SSV). Freestream data were obtained for the orbiter to provide a baseline for evaluation of proximity effects. Data were obtained at Mach numbers from 0.32 to 1.1, and Reynolds number per foot varying from 2.1 million to 3.9 million. Data are not presented. Because of balance failure, a very substantial portion of the test was run with a dummy balance in the tank boosters configuration.

  8. An investigation in the MSFC TWT to determine spoiler effects on wing loads and elevon hinge moments utilizing 0.004-scale models (77-0 and 74-OTS) of the shuttle vehicle 5 configuration (IA125)

    NASA Technical Reports Server (NTRS)

    Allen, E. C.

    1976-01-01

    Information is presented for wind tunnel tests (IA125) of a 0.004-scale orbiter, external tank, and solid rocket motor integrated vehicle model (77-0 and 74-OTS) in the MSFC Trisonic Wind Tunnel. These tests were conducted in support of MCR's 1344 and 1346. Data from these tests provide spoiler effects on wing bending/torsion and elevon hinge moments, elevon effectiveness data and the influence of solid plumes from Mach numbers of 0.6 through 2.74 at angles of attack and sideslip from -10 through 10 degrees.

  9. Crystallization and preliminary X-ray crystallographic studies of the biotin carboxyl carrier protein and biotin protein ligase complex from Pyrococcus horikoshii OT3

    SciTech Connect

    Bagautdinov, Bagautdin; Matsuura, Yoshinori; Bagautdinova, Svetlana; Kunishima, Naoki

    2007-04-01

    A truncated form of biotin carboxyl carrier protein containing the C-terminal half fragment (BCCPΔN76) and the biotin protein ligase (BPL) with the mutation R48A (BPL*) or the double mutation R48A K111A (BPL**) were successfully cocrystallized in the presence of ATP and biotin. The BPL*–BCCPΔN76 and BPL**–BCCPΔN76 crystals belong to space group P2{sub 1} and diffract X-rays to 2.7 and 2.0 Å resolution, respectively. Biotin protein ligase (BPL) catalyses the biotinylation of the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase. To elucidate the exact details of the protein–protein interactions in the biotinylation function, the C-terminal half fragment of BCCP (BCCPΔN76), the R48A mutant of BPL (BPL*) and the R48A K111A double mutant of BPL (BPL**), all of which are from Pyrococcus horikoshii OT3, have been expressed, purified and successfully cocrystallized. Cocrystals of the BPL*–BCCPΔN76 and BPL**–BCCPΔN76 complexes as well as crystals of BPL*, BPL** and BCCPΔN76 were obtained by the oil-microbatch method using PEG 20 000 as a precipitant at 295 K. Complete X-ray diffraction data sets for BPL*–BCCPΔN76 and BPL**–BCCPΔN76 crystals were collected at 100 K to 2.7 and 2.0 Å resolution, respectively, using synchrotron radiation. They belong to the monoclinic space group P2{sub 1}, with similar unit-cell parameters a = 69.85, b = 63.12, c = 75.64 Å, β = 95.9°. Assuming two subunits of the complex per asymmetric unit gives a V{sub M} value of 2.45 Å{sup 3} Da{sup −1} and a solvent content of 50%.

  10. Aerodynamic results of a separation effects test on a 0.010-scale model (52-OTS) of the integrated SSV in the AEDC/VKF 40-by-40 inch supersonic wind tunnel A (IA111), volume 1

    NASA Technical Reports Server (NTRS)

    Chee, E.

    1976-01-01

    Graphical data obtained during experimental wind tunnel aerodynamic investigations of a 0.010 scale model (52-OTS) of the integrated space shuttle vehicle was presented. The purpose of this investigation was to obtain data with the solid rocket booster (SRB) in proximity to the orbiter/external tank (O/ET), over a large O/ET initial angle of attack and sideslip range, as well as data on the SRB alone (greatly separated from the O/ET). A captive trajectory system, which supported the SRB, was used with the tunnel primary sector (supporting the O/ET) to obtain grid type separation effects data. One symmetrical SRB model was used interchangeably to obtain right-hand and left-hand SRB data. The entire investigation was conducted at a free-stream Mach number of 4.5 at unit Reynolds number of 3.95 and 5.9 million per foot.

  11. Results of an investigation of the space shuttle integrated vehicle aerodynamic heating characteristics obtained using the 0.0175-scale model 60-OTS in AEDC tunnel A during tests IH41 and IH41A

    NASA Technical Reports Server (NTRS)

    Cummings, J. W.; Dye, W. H.

    1977-01-01

    A thin skin thermocouple test was conducted to obtain heat-transfer data on the space shuttle integrated vehicle during the ascent phase of the flight profile. The test model was the 0.0175-scale thin skin thermocouple model (60-OTS) of the Rockwell International vehicle 5 configuration. The test was conducted at nominal Mach numbers of 2.5, 3.5, 4.5, and 5.5, and a free stream unit Reynolds number of 5 million per ft. Heat transfer data were obtained for angles of attack of 0, + or - 5, and 10 deg and yaw angles of 0, 3, and 6 deg. The integrated vehicle model was tested with the external tank configured with both a smooth ogive nose and an ogive nose with a spherical nose tip (nipple nose). The remainder of the test was conducted with the external tank installed alone in the tunnel.

  12. Heat transfer tests of an 0.006-scale thin-skin space shuttle thermocouple model (41-OTS) in the Langley Research Center unitary plan wind tunnel at M equals 3.7 (IH16)

    NASA Technical Reports Server (NTRS)

    Walstad, D. G.

    1975-01-01

    The results are presented of supersonic heat transfer tests performed on the .006 scale space shuttle vehicle model (41-OTS) in the Langley Research Center Unitary Plan Wind Tunnel. These tests were conducted to parametrically investigate ascent heating of the integrated vehicle and its components. The tests were conducted at a nominal Mach number of 3.7 and Reynolds numbers per foot of 2 and 5 million. The model configurations investigated were the integrated vehicle and each component alone (i.e. orbiter, tank and SRB). All the configurations were run with and without transition strips and through an angle of attack range of 0 deg to minus 5 deg with the exception of the SRB which was tested through an angle of attack range of minus 5 deg to 90 deg. The heat transfer data were obtained from 223 iron constantan thermocouples attached to stainless steel thin-skin areas of the model.

  13. Investigations of the 0.020-scale 88-OTS Integrated Space Shuttle Vehicle Jet-Plume Model in the NASA/Ames Research Center 11 by11-Foot Unitary Plan Wind Tunnel (IA80). Volume 1

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    The results are documented of jet plume effects wind tunnel test of the 0.020-scale 88-OTS launch configuration space shuttle vehicle model in the 11 x 11 foot leg of the NASA/Ames Research Center Unitary Plan Wind Tunnel. This test involved cold gas main propulsion system (MPS) and solid rocket motor (SRB) plume simulations at Mach numbers from 0.6 to 1.4. Integrated vehicle surface pressure distributions, elevon and rudder hinge moments, and wing and vertical tail root bending and torsional moments due to MPS and SRB plume interactions were determined. Nozzle power conditions were controlled per pretest nozzle calibrations. Model angle of attack was varied from -4 deg to +4 deg; model angle of sideslip was varied from -4 deg to +4 deg. Reynolds number was varied for certain test conditions and configurations, with the nominal freestream total pressure being 14.69 psia. Plotted force and pressure data are presented.

  14. Results of investigations conducted in the LaRC 4-foot unitary plan wind tunnel leg no. 1 using the 0.010-scale 72-OTS model of the space shuttle integrated vehicle (IA94A)

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    Aero-loads investigations were conducted on the updated configuration-5 space shuttle launch vehicle at Mach numbers 2.50, 3.50, and 4.50. Six-component vehicle forces and moments, base and sting-cavity pressures, elevon hinge moments, wing-root bending and torsion moments, and normal shear force data were obtained. Full simulation of updated vehicle protuberances and attach hardware was employed. Various elevon deflection angles were tested, with two different forward orbiter-to-external-tank attach-strut configurations. The entire vehicle model 72-OTS was supported by means of a balance mounted in the orbiter through its base and suspended from an appropriate sting for the specific tunnel.

  15. An experimental determination in Calspan Ludwieg tube of the base environment of the integrated space shuttle vehicle at simulated Mach 4.5 flight conditions (test IH5 of model 19-OTS)

    NASA Technical Reports Server (NTRS)

    Drzewiecki, R. F.; Foust, J. W.

    1976-01-01

    A model test program was conducted to determine heat transfer and pressure distributions in the base region of the space shuttle vehicle during simulated launch trajectory conditions of Mach 4.5 and pressure altitudes between 90,000 and 210,000 feet. Model configurations with and without the solid propellant booster rockets were examined to duplicate pre- and post-staging vehicle geometries. Using short duration flow techniques, a tube wind tunnel provided supersonic flow over the model. Simultaneously, combustion generated exhaust products reproduced the gasdynamic and thermochemical structure of the main vehicle engine plumes. Heat transfer and pressure measurements were made at numerous locations on the base surfaces of the 19-OTS space shuttle model with high response instrumentation. In addition, measurements of base recovery temperature were made indirectly by using dual fine wire and resistance thermometers and by extrapolating heat transfer measurements.

  16. Transposon Mutations in the 5′ End of glnD, the Gene for a Nitrogen Regulatory Sensor, That Suppress the Osmosensitive Phenotype Caused by otsBA Lesions in Escherichia coli

    PubMed Central

    Tøndervik, Anne; Torgersen, Haakon R.; Botnmark, Hans K.; Strøm, Arne R.

    2006-01-01

    GlnD of Escherichia coli is a bifunctional signal-transducing enzyme (102.4 kDa) which uridylylates the allosteric regulatory protein PII and deuridylylates PII-UMP in response to growth with nitrogen excess or limitation, respectively. GlnD catalyzes these reactions in response to high or low levels of cytoplasmic glutamine, respectively, and indirectly directs the expression of nitrogen-regulated genes, e.g., the glnK-amtB operon. We report that chromosomal mini-Tn10 insertions situated after nucleotide number 997 or 1075 of glnD partially suppressed the osmosensitive phenotype of ΔotsBA or otsA::Tn10 mutations (defective osmoregulatory trehalose synthesis). Strains carrying these glnD::mini-Tn10 mutations either completely repressed the expression of trp::(glnKp-lacZ) or induced this reporter system to nearly 60% of the wild-type glnD level in response to nitrogen availability, an essentially normal response. This was in contrast to the much-studied glnD99::Tn10 mutation, which carries its insertion in the 3′ end of the gene, causes a complete repression of glnKp-lacZ expression under all growth conditions, and also confers leaky glutamine auxotrophy. When expressed from the Pm promoter in plasmid constructs, the present glnD mutations produced proteins with an apparent mass of 39 or 42 kDa. These proteins were deduced to comprise 344 or 370 N-terminal residues, respectively, harboring the known nucleotidyltransferase domain of GlnD, plus a common C-terminal addition of 12 residues encoded by IS10. They lacked three other domains of GlnD. Apparently, the transferase domain by itself enabled the cells to catalyze the uridylylation reaction and direct nitrogen-regulated gene expression. Our data indicate that there exists a link between osmotic stress and the nitrogen response. PMID:16740928

  17. Aerodynamic results of a separation effects test on a 0.01-scale model (52-OTS) of integrated SSV in the AEDC/VKF 40-by-40 inch supersonic wind tunnel A, volume 1

    NASA Technical Reports Server (NTRS)

    Campbell, J. H., II

    1975-01-01

    Experimental aerodynamic investigations were conducted, during the period July 18-19, 1974, in the AEDC/VKF Tunnel A facility on a 0.01-scale model (52-OTS) of the integrated space shuttle vehicle, including only one SRB. The purpose of the investigation was to obtain data for close-in proximity (SRB to orbiter/tank) effects with the orbiter/tank combination at relatively high alpha and beta attitudes, and with the SRB separation motors off. The AEDC Captive Trajectory System (CTS), which supported the SRB, was used in conjunction with the tunnel primary sector (supporting the orbiter/tank) to obtain grid type separation effects data. The one symmetrical SRB model was used interchangeably to obtain both right-hand and left-hand SRB data. Free-stream data were also obtained for the orbiter/tank and for the SRB. This data was used to provide baselines for proximity effects. The entire investigation was conducted at a free-stream Mach number of 4.5 with unit Reynolds number ranging from 4.0 to 6.5 million per foot.

  18. Expression, high-pressure refolding, purification, crystallization and preliminary X-ray analysis of a novel single-strand-specific 3′–5′ exonuclease PhoExo I from Pyrococcus horikoshii OT3

    PubMed Central

    Miyazono, Ken-ichi; Tsutsumi, Kanae; Ishino, Yoshizumi; Tanokura, Masaru

    2014-01-01

    PhoExo I is a single-strand-specific 3′–5′ exonuclease from Pyrococcus horikoshii OT3 and is thought to be involved in a Thermococcales-specific DNA-repair pathway. The recombinant PhoExo I protein was produced as inclusion bodies in Escherichia coli cells. Solubilization of the inclusion bodies was performed by the high-pressure refolding method and highly purified protein was subjected to crystallization by the sitting-drop vapour-diffusion method at 20°C. A crystal of PhoExo I was obtained in a reservoir solution consisting of 0.1 M Tris–HCl pH 8.9, 27% PEG 6000 and diffracted X-rays to 1.52 Å resolution. The crystal of PhoExo I belonged to space group H32, with unit-cell parameters a = b = 112.07, c = 202.28 Å. The crystal contained two PhoExo I molecules in the asymmetric unit. PMID:25084386

  19. Expression, high-pressure refolding, purification, crystallization and preliminary X-ray analysis of a novel single-strand-specific 3'-5' exonuclease PhoExo I from Pyrococcus horikoshii OT3.

    PubMed

    Miyazono, Ken-ichi; Tsutsumi, Kanae; Ishino, Yoshizumi; Tanokura, Masaru

    2014-08-01

    PhoExo I is a single-strand-specific 3'-5' exonuclease from Pyrococcus horikoshii OT3 and is thought to be involved in a Thermococcales-specific DNA-repair pathway. The recombinant PhoExo I protein was produced as inclusion bodies in Escherichia coli cells. Solubilization of the inclusion bodies was performed by the high-pressure refolding method and highly purified protein was subjected to crystallization by the sitting-drop vapour-diffusion method at 20°C. A crystal of PhoExo I was obtained in a reservoir solution consisting of 0.1 M Tris-HCl pH 8.9, 27% PEG 6000 and diffracted X-rays to 1.52 Å resolution. The crystal of PhoExo I belonged to space group H32, with unit-cell parameters a = b = 112.07, c = 202.28 Å. The crystal contained two PhoExo I molecules in the asymmetric unit. PMID:25084386

  20. Results of aeroheating DFI and ET design-data test on a 0.0175-scale model 60-OTS conducted in the Von Karman Gas Dynamics Facility (VKF) 40" supersonic and the 50" hypersonic wind tunnels A and C

    NASA Technical Reports Server (NTRS)

    Marroquin, J.; Leef, C. R.

    1985-01-01

    The 0.0175-scale thin-skin thermocouple Model 60-OTS was tested in the von Karman Gas Facility 40-inch continuous flow supersonic tunnel A and the VKF Tunnel C. Testing was conducted at Mach numbers 2.25 to 4.0, and Reynolds numbers from 0.4 x 10 to the 6th power/ft to 6.6 x 10 to the 6th power/ft. Angle of attack range was from -5.0 to +5.0 degrees, and angle of sideslip range was from -6 to +6 degrees. The primary objective of this test was to provide a valid base for the external tank (ET) and solid rocket booster (SRB) heating prediction methodology for ascent flight by taking heating data at Development Flight Instrumentation (DFI) locations for flight conditions simulating STS-1 through -4. A second objective was to obtain additional aeroheating data to support potential reduction of the thermal protection system (TPS) on the ET. The third phase of the test was funded and conducted by NASA/MSFC for the purpose of establishing confidence in the data base from the lower temperature tunnel A.

  1. A new report of Nocardiopsis valliformis strain OT1 from alkaline Lonar crater of India and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity.

    PubMed

    Rathod, Dnyaneshwar; Golinska, Patrycja; Wypij, Magdalena; Dahm, Hanna; Rai, Mahendra

    2016-10-01

    The authors report the biological synthesis of silver nanoparticles (AgNPs) by alkaliphilic actinobacterium Nocardiopsis valliformis OT1 strain isolated for the first time from Lonar crater, India. The primary detection of silver NPs formation was made by colour change from colourless to dark brown and confirmed by UV-Vis spectrum of AgNPs at 423 nm, specific for AgNPs. Further, AgNPs were characterized by nanoparticle tracking analysis, Zeta sizer, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) analyses. FTIR analysis showed the presence of proteins as capping agent. TEM analysis revealed the formation of spherical and polydispersed AgNPs within the size range of 5-50 nm. The antimicrobial activity of silver nanoparticles against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis was evaluated. The AgNPs showed the maximum antibacterial activity against B. subtilis (Gram positive) and the minimum against E. coli (Gram negative). The minimal inhibitory concentration values of AgNPs for the tested bacteria were found to be in the range of 30-80 µg/mL. The AgNPs demonstrated higher antibacterial activity against all the bacteria tested as compared with the commercially available antibiotics. The cytotoxicity of biosynthesized AgNPs against in vitro human cervical cancer cell line (HeLa) demonstrated a dose-response activity. The IC50 value was found to be 100 µg/mL of AgNPs against cancer HeLa cell line. PMID:27278909

  2. Transposon mutations in the 5' end of glnD, the gene for a nitrogen regulatory sensor, that suppress the osmosensitive phenotype caused by otsBA lesions in Escherichia coli.

    PubMed

    Tøndervik, Anne; Torgersen, Haakon R; Botnmark, Hans K; Strøm, Arne R

    2006-06-01

    GlnD of Escherichia coli is a bifunctional signal-transducing enzyme (102.4 kDa) which uridylylates the allosteric regulatory protein PII and deuridylylates PII-UMP in response to growth with nitrogen excess or limitation, respectively. GlnD catalyzes these reactions in response to high or low levels of cytoplasmic glutamine, respectively, and indirectly directs the expression of nitrogen-regulated genes, e.g., the glnK-amtB operon. We report that chromosomal mini-Tn10 insertions situated after nucleotide number 997 or 1075 of glnD partially suppressed the osmosensitive phenotype of DeltaotsBA or otsA::Tn10 mutations (defective osmoregulatory trehalose synthesis). Strains carrying these glnD::mini-Tn10 mutations either completely repressed the expression of trp::(glnKp-lacZ) or induced this reporter system to nearly 60% of the wild-type glnD level in response to nitrogen availability, an essentially normal response. This was in contrast to the much-studied glnD99::Tn10 mutation, which carries its insertion in the 3' end of the gene, causes a complete repression of glnKp-lacZ expression under all growth conditions, and also confers leaky glutamine auxotrophy. When expressed from the Pm promoter in plasmid constructs, the present glnD mutations produced proteins with an apparent mass of 39 or 42 kDa. These proteins were deduced to comprise 344 or 370 N-terminal residues, respectively, harboring the known nucleotidyltransferase domain of GlnD, plus a common C-terminal addition of 12 residues encoded by IS10. They lacked three other domains of GlnD. Apparently, the transferase domain by itself enabled the cells to catalyze the uridylylation reaction and direct nitrogen-regulated gene expression. Our data indicate that there exists a link between osmotic stress and the nitrogen response. PMID:16740928

  3. Results of a M = 5.3 heat transfer test of the integrated vehicle using phase-change paint techniques on the 0.0175-scale model 56-OTS in the NASA/Ames Research Center 3.5-foot hypersonic wind tunnel

    NASA Technical Reports Server (NTRS)

    Marroquin, J.

    1985-01-01

    An experimental investigation was performed in the NASA/Ames Research Center 3.5-foot Hypersonic Wind Tunnel to obtain supersonic heat-distribution data in areas between the orbiter and external tank using phase-change paint techniques. The tests used Novamide SSV Model 56-OTS in the first and second-stage ascent configurations. Data were obtained at a nominal Mach number of 5.3 and a Reynolds number per foot of 5 x 10 to the 6th power with angles of attack of 0 deg, +/- 5 deg, and sideslip angles of 0 deg and +/- 5 deg.

  4. Identity of Innovative Multifunctional Material Manufacturing Business in Latvia / Inovatīvu Daudzfunkcionālo Materiālu Ražotāju Identitāte Latvijā

    NASA Astrophysics Data System (ADS)

    Geipele, I.; Staube, T.; Ciemleja, G.; Zeltins, N.; Ekmanis, Yu.

    2015-08-01

    The publication comprises the results from the practical scientific investigation to define the profile and distinctions of the Latvian innovative multifunctional material manufacturing industry. The research is carried out by a holistic approach, including expert interviews, qualitative analysis of the official register data, practical survey, and financial data analysis. The paper seeks to give the detailed data on a technological profile of the mentioned representative companies, if there is a synergy or tough competition in the Latvian market. The current research is topical, because it is unique and does not have analogues in Latvia, and the research is timely due to correspondence with recently stated Research and Innovation Strategies for Smart Specialisation in Latvia. The main findings are associated with recognition of the factors that make impact on commercialisation of the finished goods, obtained financial results and planned directions of development of the respondent companies. Publikācija sniedz veiktā zinātniski praktiskā pētījuma rezultātus, kura ietvaros tika noteikts Latvijas inovatīvu daudzfunkcionālo materiālu ražotāju darbības profils un identitāte. Pētījumā tika izmantota kompleksās analīzes metode, kas ietvēra ekspertu intervijas, uzņēmumu oficiāli reģistrēto datu kvalitatīvo analīzi, zinātniski praktisko aptauju, kā arī pētāmo uzņēmumu finanšu darbības rezultātu analīzi. Zinātniskais darbs sniedz izsmeļošu informāciju par mērķa uzņēmumu tehnoloģisko profilu un pēta, vai Latvijas mērķa ražošanas nozarē ir sinerģijas vai sīvās konkurences pazīmes. Šim zinātniskajam pētījumam ir noteikta aktualitāte saskaņā ar tā unikalitāti Latvijas valsts mērogā un atbilstība Viedās specializācijas stratēģijai. Pētījuma galvenie secinājumi ir saistīti ar uzņēmumu produkcijas komercializācijas ietekmējošo faktoru apzināšanu, sasniegtajiem finansiālajiem rezultātiem un pl

  5. Complete genome sequence of Haliscomenobacter hydrossis type strain (OT)

    SciTech Connect

    Daligault, Hajnalka E.; Lapidus, Alla L.; Zeytun, Ahmet; Nolan, Matt; Lucas, Susan; Glavina Del Rio, Tijana; Tice, Hope; Cheng, Jan-Fang; Tapia, Roxanne; Han, Cliff; Goodwin, Lynne A.; Pitluck, Sam; Liolios, Konstantinos; Pagani, Ioanna; Ivanova, N; Huntemann, Marcel; Mavromatis, K; Mikhailova, Natalia; Pati, Amrita; Chen, Amy; Palaniappan, Krishna; Land, Miriam L; Hauser, Loren John; Brambilla, Evelyne-Marie; Rohde, Manfred; Verbarg, Susanne; Goker, Markus; Bristow, James; Eisen, Jonathan; Markowitz, Victor; Hugenholtz, Philip; Kyrpides, Nikos C; Klenk, Hans-Peter; Woyke, Tanja

    2011-01-01

    Haliscomenobacter hydrossis van Veen et al. 1973 is the type species of the genus Halisco- menobacter, which belongs to order 'Sphingobacteriales'. The species is of interest because of its isolated phylogenetic location in the tree of life, especially the so far genomically un- charted part of it, and because the organism grows in a thin, hardly visible hyaline sheath. Members of the species were isolated from fresh water of lakes and from ditch water. The genome of H. hydrossis is the first completed genome sequence reported from a member of the family 'Saprospiraceae'. The 8,771,651 bp long genome with its three plasmids of 92 kbp, 144 kbp and 164 kbp length contains 6,848 protein-coding and 60 RNA genes, and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  6. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... disclosed under the Freedom of Information Act, see 5 U.S.C. 552, and 31 CFR 1.1-1.6; and (iv) Requests for... charged fees in accordance with Treasury Department regulations, 31 CFR 1.7. With certain exceptions, the regulations in 31 CFR 1.7 provide for recovery of the full direct costs of searching, reviewing,...

  7. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Act, see 5 U.S.C. 552, and 31 CFR 1.1-1.6. (b) Purpose. The purposes of this section are: (1) To... Department regulations, 31 CFR 1.7. With certain exceptions, the regulations in 31 CFR 1.7 provide for.... Examples of unpublished information include: (i) Information in the memory of a current or former...

  8. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... disclosed under the Freedom of Information Act, see 5 U.S.C. 552, and 31 CFR 1.1-1.6; and (iv) Requests for... charged fees in accordance with Treasury Department regulations, 31 CFR 1.7. With certain exceptions, the regulations in 31 CFR 1.7 provide for recovery of the full direct costs of searching, reviewing,...

  9. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... disclosed under the Freedom of Information Act, see 5 U.S.C. 552, and 31 CFR 1.1-1.6; and (iv) Requests for... charged fees in accordance with Treasury Department regulations, 31 CFR 1.7. With certain exceptions, the regulations in 31 CFR 1.7 provide for recovery of the full direct costs of searching, reviewing,...

  10. OTS cash disbursement process for HANDI 2000 business management system

    SciTech Connect

    Wilson, D.

    1998-08-24

    Payments by check are disbursed either through the payroll system or the Accounts Payable Master. Payments to persons with a valid employee number are processed through the payroll system. Payments to persons without valid employee number are processed through APM.