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Sample records for laboratory rearing technique

  1. Mass rearing history negatively affects mating success of male Anastrepha ludens (Diptera: Tephritidae) reared for sterile insect technique programs.

    PubMed

    Rull, Juan; Brunel, Odette; Mendez, Maria Elena

    2005-10-01

    Mating competitiveness and sterility induction into cohorts of wild Anastrepha ludens (Loew) (Diptera: Tephritidae) was compared among wild and laboratory flies reared for use in the sterile insect technique Mexican program. Laboratory flies stemming from an 11-yr-old bisexual strain were either not irradiated, irradiated at 3 krad (low dose), or irradiated at 8 krad. In 30 by 30 by 30-cm Plexiglas cages, where a cohort of laboratory flies (male and female) irradiated at different doses (0, 3, and 8 krad) was introduced with a cohort of wild flies, males and females of each type mated randomly among themselves. Compared with nonirradiated laboratory and wild males, irradiated males, irrespective of dose (3 or 8 krad), induced shorter refractory periods and greater mating frequency in wild females. Nevertheless, laboratory flies irradiated at a low dose induced greater sterility into cohorts of wild flies than laboratory flies irradiated at a high dose. In a 3 by 3 by 3-m walk-in cage, wild males gained significantly more matings with wild females than nonirradiated and irradiated laboratory males a finding that revealed a strong effect of strain on mating performance. Mating incompatibility of the laboratory strain might have obscured the effect of reduced irradiation doses on male mating performance in the walk-in cage. Our results highlight an urgent need to replace the A. ludens strain currently used by the Mexican fruit fly eradication campaign and at least suggest that reducing irradiation doses result in an increase in sterility induction in wild populations. PMID:16334318

  2. Dynamics of genetic variability in Anastrepha fraterculus (Diptera: Tephritidae) during adaptation to laboratory rearing conditions

    PubMed Central

    2014-01-01

    Background Anastrepha fraterculus is one of the most important fruit fly plagues in the American continent and only chemical control is applied in the field to diminish its population densities. A better understanding of the genetic variability during the introduction and adaptation of wild A. fraterculus populations to laboratory conditions is required for the development of stable and vigorous experimental colonies and mass-reared strains in support of successful Sterile Insect Technique (SIT) efforts. Methods The present study aims to analyze the dynamics of changes in genetic variability during the first six generations under artificial rearing conditions in two populations: a) a wild population recently introduced to laboratory culture, named TW and, b) a long-established control line, named CL. Results Results showed a declining tendency of genetic variability in TW. In CL, the relatively high values of genetic variability appear to be maintained across generations and could denote an intrinsic capacity to avoid the loss of genetic diversity in time. Discussion The impact of evolutionary forces on this species during the adaptation process as well as the best approach to choose strategies to introduce experimental and mass-reared A. fraterculus strains for SIT programs are discussed. PMID:25471362

  3. Captive-rearing piping plovers: Developing techniques to augment wild populations

    USGS Publications Warehouse

    Powell, A.N.; Cuthbert, F.J.; Wemmer, L.C.; Doolittle, A.W.; Feirer, S.T.

    1997-01-01

    Techniques for captive-rearing and releasing piping plovers (Charadrius melodus) were developed using a surrogate species, killdeer (Charadrius vociferus). We compared captive-and parent-reared killdeer, and parent-reared piping plovers and determined that growth and behavior were similar. After surrogate trials determined that captive-rearing was feasible, we used the same methods to raise piping plover chicks from salvaged eggs. For captive-reared chick of both species, survival to fledging was higher than and behaviors similar to parent-reared chicks in the wild. Rearing techniques were fine-tuned, and ten piping plover fledglings were released to the wild. Based on our results, we developed recommendations for captive-rearing piping plovers using salvaged eggs to enhance productivity of small populations. ?? 1997 Wiley-Liss, Inc.

  4. Captive-rearing piping plovers: developing techniques to augment wild populations

    USGS Publications Warehouse

    Powell, A.N.; Cuthbert, F.J.; Wemmer, L.C.; Doolittle, A.

    1997-01-01

    Techniques for captive-rearing and releasing piping plovers (Charadrius melodus) were developed using a surrogate species, killdeer (Charadrius vociferus). We compared captive- and parent-reared killdeer, and parent-reared piping plovers and determined that growth and behavior were similar. After surrogate trials determined that captive-rearing was feasible, we used the same methods to raise piping plover chicks from salvaged eggs. For captive-reared chick of both species, survival to fledging was higher than and behaviors similar to parent-reared chicks in the wild. Rearing techniques were fine-tuned, and ten piping plover fledglings were released to the wild. Based on our results, we developed recommendations for captive-rearing piping plovers using salvaged eggs to enhance productivity of small populations.

  5. Phenotypic changes in laboratory-reared colonies of the maize herbivore, Diabrotica virgifera virgifera

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The North American and European maize pest Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) was used to assess whether environmental conditions of the natal field, subsequent laboratory rearing, or genetic population characteristics affect phenotypic traits of fitness, activity, or...

  6. Methods for rearing scutacarid mites (Acari, Heterostigmatina) and the influence of laboratory cultures on morphometric variables.

    PubMed

    Jagersbacher-Baumann, Julia; Ebermann, Ernst

    2013-04-01

    Mites of the soil inhabiting family Scutacaridae (Heterostigmatina) are distributed throughout the world, but only rarely found in high densities. Larvae and males are extremely difficult to detect and identify in soil samples. Laboratory cultures are necessary to describe these life stages, detect female dimorphism, or carry out other kinds of biological study. The present paper gives an historical overview of the methods applied for rearing scutacarids, demonstrating that thus far the use of glass tubes stuffed with soil is the most valuable method. Morphometric comparisons between field collected and laboratory reared specimens of two scutacarid species, Scutacarus acarorum Goeze and Heterodispus foveatus Jagersbacher-Baumann and Ebermann, revealed a clear influence of environmental conditions on the phenotype of laboratory-reared mites. Size correction minimized the environmentally induced variation and should therefore be mandatory for classification of species based on morphometric variables. Taxonomic assignment of laboratory reared scutacarids is possible, but must be done with extreme caution. PMID:23007228

  7. Aggression and feeding of hatchery-reared and naturally reared steelhead (Oncorhynchus mykiss) fry in a laboratory flume and a comparison with observations in natural streams

    USGS Publications Warehouse

    Riley, S.C.; Tatara, C.P.; Scheurer, J.A.

    2005-01-01

    We quantified the aggression and feeding of naturally reared steelhead (Oncorhynchus mykiss) fry stocked into a laboratory flume with naturally reared fry or hatchery-reared fry from conventional and enriched rearing environments at three densities in the presence and absence of predators, and compared the aggression and feeding observed in the flume to that observed in two streams. Steelhead fry attack rate increased with density and was reduced in the presence of predators, but was not affected by rearing treatment. Threat rate appeared to increase with density and was significantly affected by rearing treatment combination, but was not significantly affected by predator presence. Feeding rate was not affected by density or rearing treatment, but was reduced in the presence of predators. The rate of aggression by steelhead fry in two streams was lower than that observed in the laboratory and did not increase with density. Rates of aggression and feeding of hatchery-reared and wild steelhead fry were not significantly different in the streams. Overall, we found no evidence that hatchery rearing environments caused higher aggression in steelhead fry. Laboratory observations of salmonid aggression, particularly at high density, may not reflect aggression levels in the wild. ?? 2005 NRC.

  8. Development of mass rearing technique of Tyrophagus putrescentiae (Acari: Acaridae) found in house dust.

    PubMed

    Ree, H I; Lee, I Y

    1997-09-01

    A storage mite, Tyrophagus putrescentiae, is recently known to be widely distributed in Korea, being commonly found in house dust, and may, therefore, be allergenically important. The purpose of this study was to develop mass rearing techniques for supplying a large quantity of allergens. The laboratory mouse food powder gave the highest yield, showing 1,251.5-fold increase in number after 10 weeks, and the mixed powder of laboratory mouse food and yeast (1:1) also gave same level of the production (1,203.1-fold increase in week 10). Several different combinations of temperature and relative humidity conditions were compared, and the maximum propagation was obtained at 25 degrees C and 64% RH, showing 960-fold increase in number. When the same amount of culture media was used, the size of the culture container did not significantly influence the quantitative yield of T. putrescentiae mites. PMID:9335179

  9. Rearing Africanized honey bee (Apis mellifera L.) brood under laboratory conditions.

    PubMed

    Silva, I C; Message, D; Cruz, C D; Campos, L A O; Sousa-Majer, M J

    2009-01-01

    We developed a method for rearing larvae of Africanized bees under laboratory conditions to determine the amount of diet needed during larval development to obtain a worker bee. We started with larvae 18-24 h old, which were transferred to polyethylene cell cups and fed for five days. We found that the amount of diet needed for successful larval development was: 4, 15, 25, 50, and 70 microl during the first to fifth days, respectively. The survival rate to the adult stage was 88.6% when the larvae received the daily amount of diet divided into two feedings, and 80% when they received only one feeding per day. The adult weight obtained in the laboratory, when the larvae received the daily amount of diet in a single dose, did not differ from those that were developed under field conditions (our control). All adults that we obtained in laboratory appeared to be normal. This technique has the potential to facilitate studies on brood pathogens, resistance mechanisms to diseases and also might be useful to test the impacts of transgenic products on honey bee brood. PMID:19551650

  10. Comparative Studies of Predation Among Feral, Commercially-Purchased, and Laboratory-Reared Predators

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The predatory activities of commercially-purchased Hippodamia convergens Guèrin-Mèneville and two laboratory-reared strains of Geocoris punctipes (Say) were compared with their feral counterparts. In single prey choice feeding tests, commercially-purchased and feral H. convergens were provided copi...

  11. LIGHT INTENSITY AFFECTS DISTRIBUTION OF ATTACKING PSEUDACTEON CURVATUS (DIPTERA: PHORIDAE) IN A LABORATORY REARING SYSTEM

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Distribution of attacking phorid flies (Pseudacteon curvatus Borgmeier) in a laboratory rearing system was tested for dependence on light intensity under 2 different light regimes. Light intensity (range, approx. 220-340 Lux) influenced fly distribution in light regime 1; in the second light regime...

  12. Performance of Wild and Laboratory-Reared Gypsy Moth (Lepidoptera: Erebidae): A Comparison between Foliage and Artificial Diet.

    PubMed

    Grayson, Kristine L; Parry, Dylan; Faske, Trevor M; Hamilton, Audrey; Tobin, Patrick C; Agosta, Salvatore J; Johnson, Derek M

    2015-06-01

    The effects of long-term mass rearing of laboratory insects on ecologically relevant traits is an important consideration when applying research conclusions to wild populations or developing management strategies. Laboratory strains of the gypsy moth, Lymantria dispar (L.), an invasive forest pest in North America, have been continuously reared since 1967. Selection on these strains has enhanced a variety of traits, resulting in faster development, shorter diapause, and greater fecundity. As in many mass-reared insects, laboratory strains of the gypsy moth are also reared exclusively on artificial diets that lack much of the phytochemical and nutritional complexity associated with natural foliage. We tested for differences in growth and development of wild gypsy moth populations from across the invasive range in comparison to laboratory strains when reared on artificial diet and a preferred foliage host species, northern red oak (Quercus rubra L.). Overall, caterpillars reared on foliage had higher survival and faster development rates, with smaller differences among populations. When reared on artificial diet, laboratory strains had the highest performance as expected. The response from the wild populations was mixed, with two populations performing poorly on artificial diet and another performing nearly as well as the laboratory strains. Performance on diet was enhanced when larvae received cubed portions changed regularly, as opposed to filled cups. Understanding these relationships between food source and population performance is important for informing studies that examine population comparisons using wild and laboratory-reared strains. PMID:26313993

  13. [Occurrence of the mite Pyemotes sp. (Acari: Pyemotidae) in insect rearing in laboratory].

    PubMed

    da Cunha, Uemerson S; Silva, Edmilson S; de Moraes, Gilberto J; Vendramim, José D

    2006-01-01

    Pyemotidae mites have been reported as ectoparasites of a large number of arthropods, especially of insects. These mites are frequently found attacking insects in different habitats, including insect rearing. This paper reports the occurrence of Pyemotes sp., ventricosus group, in colonies of Tuta absoluta (Meyerick) (Lepidoptera: Gelechiidae), Anagasta kuehniella (Zeller) (Lepidoptera: Pyralidae) and Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), in the insect rearing laboratory of ESALQ-USP, in Piracicaba, State of São Paulo, provoking dematitis in those involved with the rearing. This is the first report of a Pyemotes species parasitizing T. absoluta and S. zeamais. Considering the capacity of Pyemotes to cause dermatitis in human beings, demonstrated by other authors, the direct use of the Pyemotes species found in this work for the control of the reported insect species may not be promising. PMID:17061810

  14. Laboratory rearing of wild Arctic cod Boreogadus saida from egg to adulthood.

    PubMed

    Kent, D; Drost, H E; Fisher, J; Oyama, T; Farrell, A P

    2016-03-01

    The techniques and protocols used to successfully capture, transport and breed Arctic cod Boreogadus saida, as well as to rear their larvae through to adulthood are summarized. Breeding B. saida will increase the opportunity to study this fish species, which is a critical part of the Arctic food web. PMID:26832071

  15. Effect of phloem thickness on heterozygosity in laboratory-reared mountain pine beetles. Forest Service research note

    SciTech Connect

    Amman, G.D.; Stock, M.W.

    1995-02-01

    Mountain pine beetles (Dendrocotonus ponderosae Hopkins) were collected from naturally infested trees of lodgepole pine (Pinus contorta Dougl.) in northern Utah. Bettles were reared in logs through six generations in a laboratory, and heterozygosity measured. Heterozygosity levels initially decreased when individual pairs of beetles were reared. However, when beetles were allowed to selected mates at random, heterozygosity rose to levels higher than those in the starting population. Heterozygosity was higher in bettles reared in thin than those in thick phloem.

  16. Techniques for rearing and releasing nonmigratory cranes: Lessons from the Mississippi Sandhill Crane program

    USGS Publications Warehouse

    Ellis, D.H.; Olsen, G.H.; Gee, G.F.; Nicolich, J.M.; O'Malley, K.E.; Nagendran, M.; Hereford, Scott G.; Range, P.; Harper, W.T.; Ingram, R.P.; Smith, D.G.

    1992-01-01

    Captive-reared Mississippi sandhill cranes (Grus canadensis pulla) reared at the Patuxent Wildlife Research Center (Patuxent) have been released at the Mississippi Sandhill Crane National Wildlife Refuge (MSCNWR) since 1981. Of 131 birds released through December 1990, 103 were reared by foster parents. The remaining 28 were experimentally hand-reared in 1989 and 1990. After refining release procedures, parent-reared birds have integrated into the wild flock, many have survived, and some have bred. Releases of hand-reared cranes elsewhere in the 1970's were largely unsuccessful, at least in part due to the lack of a lengthy acclimation period. A new hand-rearing protocol holds promise in producing release-worthy birds. The technique employs some features first used in the 1960's (e.g., a costume for the human caretaker and model crane heads used to train chicks to feed). In the mid-1980's, the following features were added: (1) the costumed caretaker was given a visor and feathers, (2) a taxidermic crane head or a hand puppet was held or suspended from the ceiling for use in stimulating chicks to feed, (3) a taxidermic mount of a brooding crane supplied warmth, (4) a full-sized live crane was maintained in an adjacent pen and in visual contact with neonatal young to provide an imprinting model, and (5) a small group of adult (or subadult) cranes was penned adjacent to the outdoor chick pens to provide socialization models. Recent releases of Mississippi sandhill cranes hand-reared according to this protocol and released in Mississippi have had high first-year survival rates. The now-operational technique holds promise for producing large numbers of release-worthy birds.

  17. Toxic exposure to ethylene dibromide and mercuric chloride: effects on laboratory-reared octopuses.

    PubMed

    Adams, P M; Hanlon, R T; Forsythe, J W

    1988-01-01

    The effects of acute and chronic exposure to either ethylene dibromide (EDB) or mercuric chloride (MC) were studied in laboratory-reared Octopus joubini, O. maya and O. bimaculoides. The advantages of using octopuses were that the responses were immediate, highly visible and sensitive. All species demonstrated signs of toxicity to acute and chronic exposure to EDB and to MC. A dosage-sensitive relationship for the loss and subsequent recovery of locomotor response and of chromatophore expansion was found for each species after acute exposure. For each species the LC50 for chronic exposure occurred within 12 hr at 100 mg/l for EDB and within 3 hr at 1,000 mg/l for MC. This study demonstrated the potential usefulness of laboratory-reared octopuses in evaluating the toxicity of marine environmental pollutants. PMID:3072470

  18. Evaluating the use of irradiated caribean fruit fly (Diptera:Tephritidae) larvae for laboratory rearing of Doryctobracon areolatus (Hymenoptera: Braconidae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Mass-rearing and augmentative release of hymenopterous parasitoids has been a component of area-wide management programs for several tephritid fruit flies, including pestiferous species of the genus Anastrepha (Cancino and Montoya, in press). Laboratory rearing of Doryctobracon areolatus (Szeplige...

  19. Rearing of the cucurbit fly Dacus cilratus Loew (Dip: Tephritidae) on artificial diet under laboratory conditions.

    PubMed

    Reza Rassoulian, Gh; Naiimi, M; Talebi, Kh

    2004-01-01

    Cucurbit fly is an important pest of cucurbit plants (Cucumber, melon and watermelon) in most mid-east countries including, Iran. This insect causes a high damage so that several sprayings are required to control the pest. Using male strile is another physical method for controlling this pest. For this purpose it is necessary to rear many male insects. Therefore an investigation was carried out to compare different artificial diets for rearing the melon fly. First, pupae were collected from cucurbit field and transferred to laboratory. In this experiment five diet formulations were compared and the following was used for rearing: Wheat bran (14 g), soybean lees (3 g) sugar (50 g), yeast extract (2.7 g) nipagin (0.1 g). sodiumbenzoate (0.1 g) Hydrochloric acid 3.5% (4.0 ml) and distilled water 70.5 mil. For rearing the adult, the best diets contained: Brower yeast (1 part), Honey (5 parts), water (94 parts) and a slice of cucumber. These diet caused abundance of oviposition and egg fertility. PMID:15759431

  20. Selectivity of pesticides used on cotton (Gossypium hirsutum) to Trichogramma pretiosum reared on two laboratory-reared hosts.

    PubMed

    Bastos, Cristina S; de Almeida, Raul P; Suinaga, Fábio A

    2006-01-01

    The side-effects of pesticides (insecticides, fungicides, herbicides and plant growth regulators) used on cotton were tested on adults and pupae of Trichogramma pretiosum Riley reared in the laboratory on two different hosts, the Angoumois grain moth (Sitotroga cerealella Olivier) and the Mediterranean flour moth (Ephestia kuehniella (Zeller)). The eggs of the host enclosing the parasitoid pupae received direct pesticide sprays, while the adults of the parasitoid were exposed to the pesticides through contact with residues on sprayed eggs offered to parasitism. Alpha-cypermethrin, carbosulfan, deltamethrin, endosulfan, profenofos and zeta-cypermethrin were highly noxious to the parasitoid, significantly reducing the percentage of emergence and parasitism of T. pretiosum developing in E. kuehniella or S. cerealella eggs. However, the pupal stage of the parasitoid developing in S. cerealella eggs was less susceptible to alpha-cypermethrin and deltamethrin. Lufenuron and metamidophos greatly reduced the percentage of adult emergence from eggs of both hosts, while novaluron only interfered on this variable when the wasps were developing in E. kuehniella eggs. However, lufenuron and monocrotophos had no effect on the parasitoid pupae of T. pretiosum developing in E. kuehniella eggs. Chlorfluazuron, diafenthiuron, diflubenzuron, fentin hydroxide, mepiquat chloride, novaluron, thiacloprid and triflumuron did not affect T. pretiosum emergence when eggs of S. cerealella enclosing pupae of the wasps were surface treated. The pesticides azoxystrobin, carbendazin + thiram, mepiquat chloride and novaluron had no effect on the ability of the wasps to parasitise E. kuehniella eggs. However, only mepiquat chloride did not affect the percentage of F1 wasps emerging from E. kuehniella eggs. The remaining pesticides moderately reduced the percentage of emergence and parasitism of the wasps when they had contact with the chemicals during their pupal or adult stage. Thus there were differences in pesticide toxicity according to the host used for parasitoid development. These differences were hypothesised to occur because of differences in egg morphology and parasitoid performance. PMID:16308868

  1. Molecular phylogeny, laboratory rearing, and karyotype of the bombycid moth, Trilocha varians.

    PubMed

    Daimon, Takaaki; Yago, Masaya; Hsu, Yu-Feng; Fujii, Tsuguru; Nakajima, Yumiko; Kokusho, Ryuhei; Abe, Hiroaki; Katsuma, Susumu; Shimada, Toru

    2012-01-01

    This study describes the molecular phylogeny, laboratory rearing, and karyotype of a bombycid moth, Trilocha varians (F. Walker) (Lepidoptera: Bombycidae), which feeds on leaves of Ficus spp. (Rosales: Moraceae). The larvae of this species were collected in Taipei city, Taiwan, and the Ryukyu Archipelago (Ishigaki and Okinawa Islands, Japan). Molecular phylogenetic analyses revealed that T. varians belongs to the subfamily Bombycinae, thus showing a close relationship to the domesticated silkworm Bombyx mori (L.), a lepidopteran model insect. A laboratory method was developed for rearing T. varians and the time required for development from the embryo to adult was determined. From oviposition to adult emergence, the developmental zero was 10.47 °C and total effective temperature was 531.2 day-degrees, i.e., approximately 30 days for one generation when reared at 28 °C. The haploid of T. varians consisted of n = 26 chromosomes. In highly polyploid somatic nuclei, females showed a large heterochromatin body, indicating that the sex chromosome system in T. varians is WZ/ZZ (female/male). The results of the present study should facilitate the utilization of T. varians as a reference species for B. mori, thereby leading to a greater understanding of the ecology and evolution of bombycid moths. PMID:22963522

  2. Superparasitism in laboratory rearing of Spalangia cameroni (Hymenoptera: Pteromalidae), a parasitoid of medfly (Diptera: Tephritidae).

    PubMed

    Tormos, J; Asís, J; Sabater-Muñoz, B; Baños, L; Gayubo, S F; Beitia, F

    2012-02-01

    The frequency of superparasitism and its effects on the quality of laboratory-reared Spalangia cameroni (Hymenoptera: Pteromalidae) parasitoids were investigated under laboratory conditions. Numerous variables were measured, such as the number of 'ovip holes' per host as a measure of superparasitism. Adult emergence and sex ratio, as well as female size, emergence ability from soil and longevity were also measured. Finally, an assessment was made of fertility and survival of adult parasitoids emerging from the medfly Ceratitis capitata (Diptera: Tephritidae) pupae with different levels of superparasitism. A high frequency and prevalence of superparasitism under laboratory rearing conditions was observed. The number of 'ovip holes' per host ranged from one to 17, with an average (±SD) of 2.8±3.4. Sex ratios became increasingly female-biased with increasing levels of superparasitism, although overall levels of wasp emergence (male, female) declined. Nevertheless, no relationship was discerned between female size and level of superparasitism. The 'emergence ability from the soil' was higher in those parasitoids that emerged from strongly superparasitized hosts, but not related to the type of substrate in which the host pupae were buried. The level of superparasitism did not have a significant effect on the longevity, fertility and survival of female parasitoids. Our results support the hypothesis that superparasitism in S. cameroni might be adaptive, since attributes such as 'emergence ability from the soil', longevity, fertility and survival were not affected by the level of superparasitism or the presumably detrimental effects derived from physical combats among conspecific larvae. Our findings are relevant to recommendations for rearing S. cameroni for biological control releases, as well as shedding light on superparasitism under both laboratory and field conditions. PMID:21838957

  3. Larval development of the subantarctic king crabs Lithodes santolla and Paralomis granulosa reared in the laboratory

    NASA Astrophysics Data System (ADS)

    Calcagno, J. A.; Anger, K.; Lovrich, G. A.; Thatje, S.; Kaffenberger, A.

    2004-02-01

    The larval development and survival in the two subantarctic lithodid crabs Lithodes santolla (Jaquinot) and Paralomis granulosa (Molina) from the Argentine Beagle Channel were studied in laboratory cultures. In L. santolla, larval development lasted about 70 days, passing through three zoeal stages and the megalopa stage, with a duration of approximately 4, 7, 11 and 48 days, respectively. The larval development in P. granulosa is more abbreviated, comprising only two zoeal stages and the megalopa stage, with 6, 11 and 43 days' duration, respectively. In both species, we tested for effects of presence versus absence of food (Artemia nauplii) on larval development duration and survival rate. In P. granulosa, we also studied effects of different rearing conditions, such as individual versus mass cultures, as well as aerated versus unaerated cultures. No differences in larval development duration and survival were observed between animals subjected to those different rearing conditions. The lack of response to the presence or absence of potential food confirms, in both species, a complete lecithotrophic mode of larval development. Since lithodid crabs are of high economic importance in the artisanal fishery in the southernmost parts of South America, the knowledge of optimal rearing conditions for lithodid larvae is essential for future attempts at repopulating the collapsing natural stocks off Tierra del Fuego.

  4. SIMILARITY IN RESPONSES OF LABORATORY-REARED ANED FIELD-COLLECTED LONE STAR TICK (ACARI:IXODIDAE)NYMPHS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Field testing tick repellents intended for use on human skin can be difficult, particularly when multiple concentrations of multiple repellents must be tested. Therefore, laboratory tests using laboratory reared ticks have been important. To address concerns that test results obtained with laborator...

  5. Changes associated with laboratory rearing in antennal sensilla patterns of Triatoma infestans, Rhodnius prolixus, and Rhodnius pallescens (Hemiptera, Reduviidae, Triatominae).

    PubMed

    Catalá, S S; Maida, D M; Caro-Riaño, H; Jaramillo, N; Moreno, J

    2004-02-01

    We examined changes in the array of antennal sensilla of three species of Triatominae (Triatoma infestans, Rhodnius prolixus, and R. pallescens) following their establishment for different periods in laboratory culture. In each case, the laboratory colonies were compared with conspecific samples taken directly from the field, by quantitative analysis of the sensilla arrays on the three distal segments of the antenna in terms of the densities of three types of chemoreceptors (basiconics and thick and thin walled trichoids) and one type of mechanoreceptor (bristles). Sensilla densities were compared by ANOVA or non-parametric tests, and by multivariate discriminant analysis. Strains of the same species reared in different laboratories showed significant differences in their sensilla arrays, especially when compared to field-collected material from the same geographic origin. A Bolivian strain of T. infestans reared in the laboratory for 15 years and fed at monthly intervals, showed greatest differences from its conspecific wild forms, especially in terms of reductions in the number of chemoreceptors. By contrast, an Argentine strain of T. infestans reared for 25 years in the laboratory and fed weekly, showed a relative increase in the density of mechanoreceptors. A Colombian strain of R. prolixus reared for 20 years and fed weekly or fortnightly, showed only modest differences in the sensilla array when compared to its wild populations from the same area. However, a Colombian strain of R. pallescens reared for 12 years and fed fortnightly, did show highly significant reductions in one form of chemoreceptor compared to its conspecific wild populations. For all populations, multivariate analysis clearly discriminated between laboratory and field collected specimens, suggesting that artificial rearing can lead to modifications in the sensory array. This not only supports the idea of morphological plasticity in these species, but also suggests caution in the use of long-established laboratory material for experimental studies designed to extrapolate the natural behaviour and physiology of these species. PMID:15057343

  6. Life history of a free-living marine nematode Daptonema normandicum reared in laboratory.

    PubMed

    Singh, Ravail; Ingole, Baban

    2011-03-01

    Life history of a free-living meiobenthic nematode Daptonema normandicum (DeMan, 1890) was studied in the laboratory. Live specimens were primarily collected from the sewage outlet site near the mouth of the Mandovi estuary, Goa This species was the most dominant (> 67%) among the meiobenthic nematodes. Vertically, nematode abundance was highest at the surface sediment and correlated with the organic carbon and sediment chlorophyll-a. Considering their dominance in the meiofauna, attempts were made to rear D. normandicum in laboratory. Salinity of the culture medium was maintained at 14 to 17 PSU (same as the collection site). All the culture experiments were conducted in semisolid nutrient agar media at 27 +/- 2 degrees C temperature for 12 hr dark: 12 hr light conditions. The food consists primarily of an unidentified bacterium and mixed algae, but diatom and ciliates were also observed in culture. Females produced first batch of eggs at the age of 23 days. Gravid female normally carry 8-10 eggs. Embryonic development is completed in -72 hr and entire life cycle (egg to adult) was completed in 22-24 days. Average size of juveniles at the hatching was 0.189 mm. Young individuals attains a maximum size of 1.23 mm (male) and 1.04 mm (female) in -21-23 days. Growth, in terms of length was augmented upto 23rd day and ceased thereafter. The daily growth increment for the first 5 days was 0.01-0.04 mm which increased upto 0.05-0.08 mm d(-1) during the maturation (10-18 days). Male : female ratio was 1:2. In this laboratory study, we provided information on the embryonic development, the life cycle and ecology Our results demonstrated that D. normandicum can be reared successfully under the controlled conditions, suggesting possible use of this species in toxicological and aquaculture studies. The culture method described is very handy and can be applicable for rearing other meiobenthic species particularly the nematodes with comparable feeding habits. PMID:21882647

  7. Viruses in laboratory-reared cactus moth, Cactoblastis cactorum (Lepidoptera: Pyralidae)

    SciTech Connect

    Marti, O.G.; Myers, R.E.; Carpenter, J.E.; Styer, E.L.

    2007-03-15

    The cactus moth, Cactoblastis cactorum (Lepidoptera: Pyralidae: Phycitinae), is a non-native species threatening a variety of native cacti, particularly endangered species of Opuntia (Zimmerman et al. 2001), on the coast of the Gulf of Mexico. Cactoblastis cactorum populations have expanded from Florida northward along the Atlantic coast as far as Charleston, SC, and westward along the Gulf of Mexico to Dauphin Island, south of Mobile, AL. It is feared that further movement to the west will allow C. cactorum to enter the US desert Southwest and Mexico, particularly the latter. Numerous cactus species, especially those of the genera Opuntia and Nopalea, are native to the U.S. and Mexico. Local economies based on agricultural and horticultural uses of cacti could be devastated by C. cactorum (Vigueras and Portillo 2001). A bi-national control program between the US and Mexico is being developed, utilizing the sterile insect technique (SIT). In the SIT program, newly emerged moths are irradiated with a {sup 60}Co source and released to mate with wild individuals. The radiation dose completely sterilizes the females and partially sterilizes the males. When irradiated males mate with wild females, the F1 progeny of these matings are sterile. In order for the SIT program to succeed, large numbers of moths must be reared from egg to adult on artificial diet in a quarantined rearing facility (Carpenter et al. 2001). Irradiated insects must then be released in large numbers at the leading edge of the invasive population and at times which coincide with the presence of wild individuals available for mating. Mortality from disease in the rearing colony disrupts the SIT program by reducing the numbers of insects available for release.

  8. Growth and reproduction of laboratory-reared neanurid Collembola using a novel slime mould diet

    NASA Astrophysics Data System (ADS)

    Hoskins, Jessica L.; Janion-Scheepers, Charlene; Chown, Steven L.; Duffy, Grant A.

    2015-07-01

    Although significant progress has been made using insect taxa as model organisms, non-tracheated terrestrial arthropods, such as Collembola, are underrepresented as model species. This underrepresentation reflects the difficulty in maintaining populations of specialist Collembola species in the laboratory. Until now, no species from the family Neanuridae have been successfully reared. Here we use controlled growth experiments to provide explicit evidence that the species Neanura muscorum can be raised under laboratory conditions when its diet is supplemented with slime mould. Significant gains in growth were observed in Collembola given slime mould rather than a standard diet of algae-covered bark. These benefits are further highlighted by the reproductive success of the experimental group and persistence of laboratory breeding stocks of this species and others in the family. The necessity for slime mould in the diet is attributed to the ‘suctorial’ mouthpart morphology characteristic of the Neanuridae. Maintaining laboratory populations of neanurid Collembola species will facilitate their use as model organisms, paving the way for studies that will broaden the current understanding of the environmental physiology of arthropods.

  9. Growth and reproduction of laboratory-reared neanurid Collembola using a novel slime mould diet

    PubMed Central

    Hoskins, Jessica L.; Janion-Scheepers, Charlene; Chown, Steven L.; Duffy, Grant A.

    2015-01-01

    Although significant progress has been made using insect taxa as model organisms, non-tracheated terrestrial arthropods, such as Collembola, are underrepresented as model species. This underrepresentation reflects the difficulty in maintaining populations of specialist Collembola species in the laboratory. Until now, no species from the family Neanuridae have been successfully reared. Here we use controlled growth experiments to provide explicit evidence that the species Neanura muscorum can be raised under laboratory conditions when its diet is supplemented with slime mould. Significant gains in growth were observed in Collembola given slime mould rather than a standard diet of algae-covered bark. These benefits are further highlighted by the reproductive success of the experimental group and persistence of laboratory breeding stocks of this species and others in the family. The necessity for slime mould in the diet is attributed to the ‘suctorial’ mouthpart morphology characteristic of the Neanuridae. Maintaining laboratory populations of neanurid Collembola species will facilitate their use as model organisms, paving the way for studies that will broaden the current understanding of the environmental physiology of arthropods. PMID:26153104

  10. An unusual case of coccidiosis in laboratory-reared pheasants resulting from a breach in biosecurity.

    PubMed

    Gerhold, R W; Williams, S M; Fuller, A L; McDougald, L R

    2010-09-01

    An outbreak of coccidiosis in laboratory-reared Chinese ring-necked pheasants (Phasianus colchicus) resulted in high morbidity and moderate mortality. The outbreak was associated with a breach in biosecurity caused by the cleaning of a sewer line with a mechanical device, resulting in extensive splattering of fecal material throughout the "clean room" where birds were held prior to use in coccidiosis experiments. Mortality and morbidity in the affected birds were seen exactly 5 days after the incident, after birds had been moved to another room for experimental use, corresponding closely with the known prepatent or preclinical period of Eimeria phasiani and Eimeria colchici. Gross lesions in the affected birds varied from dehydration to intestinal and ventricular hemorrhage. Microscopic examination confirmed a diagnosis of severe intestinal coccidiosis. This report underscores the ease of contamination of experimental birds leading to coccidiosis outbreaks during breaches of management and biosecurity. PMID:20945799

  11. Airborne Synthetic Aperature Radar (AIRSAR) on left rear fuselage of DC-8 Airborne Laboratory

    NASA Technical Reports Server (NTRS)

    1998-01-01

    A view of the Airborne Synthetic Aperature Radar (AIRSAR) antenna on the left rear fuselage of the DC-8. The AIRSAR captures images of the ground from the side of the aircraft and can provide precision digital elevation mapping capabilities for a variety of studies. The AIRSAR is one of a number of research systems that have been added to the DC-8. NASA is using a DC-8 aircraft as a flying science laboratory. The platform aircraft, based at NASA's Dryden Flight Research Center, Edwards, Calif., collects data for many experiments in support of scientific projects serving the world scientific community. Included in this community are NASA, federal, state, academic and foreign investigators. Data gathered by the DC-8 at flight altitude and by remote sensing have been used for scientific studies in archeology, ecology, geography, hydrology, meteorology, oceanography, volcanology, atmospheric chemistry, soil science and biology.

  12. A description of the larval development of Megabalanus azoricus (Pilsbry, 1916) reared in the laboratory

    NASA Astrophysics Data System (ADS)

    Dionísio, Maria; Rodrigues, Armindo; Costa, Ana

    2014-03-01

    This study provides the first description of the larval development of the commercially exploited barnacle Megabalanus azoricus. It describes the changes in larval size and shape as well as the general morphology and duration of each larval stage. Embryos were obtained from gravid specimens collected at São Miguel Island and reared through six naupliar stages to the cypris stage in laboratory conditions. The planktotrophic nauplii reached the cypris stage after 14 days of hatching in individual cultures at 20 °C under natural illumination and fed with phytoplankton ( Chaetoceros gracilis, Isochrysis sp., and Tetraselmis sp.). The nauplius of M. azoricus has a normal size compared with nauplii of other congeneric species, ranging between the 261 μm (nauplius I) and 912 μm (nauplius VI). This work provides the first description of larvae of the genus Megabalanus for the Portuguese oceanic islands and provides comparisons with congeneric species in other parts of the world.

  13. Standard metabolism and growth dynamics of laboratory-reared larvae of Sardina pilchardus.

    PubMed

    Moyano, M; Garrido, S; Teodósio, M A; Peck, M A

    2014-04-01

    This study provides the first measurements of the standard respiration rate (R(S)) and growth dynamics of European sardine Sardina pilchardus larvae reared in the laboratory. At 15° C, the relationship between RS (µl O(2) individual(-1)  h(-1)) and larval dry mass (M(D), µg) was equal to: R(S) = 0·0057(±0·0007, ± s.e.)·M(D) (0·8835(±0·0268)), (8-11% M(D) day(-1)). Interindividual differences in RS were not related to interindividual differences in growth rate or somatic (Fulton's condition factor) or biochemical-based condition (RNA:DNA). PMID:24646016

  14. Aseptic Laboratory Techniques: Plating Methods

    PubMed Central

    Sanders, Erin R.

    2012-01-01

    Microorganisms are present on all inanimate surfaces creating ubiquitous sources of possible contamination in the laboratory. Experimental success relies on the ability of a scientist to sterilize work surfaces and equipment as well as prevent contact of sterile instruments and solutions with non-sterile surfaces. Here we present the steps for several plating methods routinely used in the laboratory to isolate, propagate, or enumerate microorganisms such as bacteria and phage. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental materials. Procedures described include (1) streak-plating bacterial cultures to isolate single colonies, (2) pour-plating and (3) spread-plating to enumerate viable bacterial colonies, (4) soft agar overlays to isolate phage and enumerate plaques, and (5) replica-plating to transfer cells from one plate to another in an identical spatial pattern. These procedures can be performed at the laboratory bench, provided they involve non-pathogenic strains of microorganisms (Biosafety Level 1, BSL-1). If working with BSL-2 organisms, then these manipulations must take place in a biosafety cabinet. Consult the most current edition of the Biosafety in Microbiological and Biomedical Laboratories (BMBL) as well as Material Safety Data Sheets (MSDS) for Infectious Substances to determine the biohazard classification as well as the safety precautions and containment facilities required for the microorganism in question. Bacterial strains and phage stocks can be obtained from research investigators, companies, and collections maintained by particular organizations such as the American Type Culture Collection (ATCC). It is recommended that non-pathogenic strains be used when learning the various plating methods. By following the procedures described in this protocol, students should be able to: ● Perform plating procedures without contaminating media. ● Isolate single bacterial colonies by the streak-plating method. ● Use pour-plating and spread-plating methods to determine the concentration of bacteria. ● Perform soft agar overlays when working with phage. ● Transfer bacterial cells from one plate to another using the replica-plating procedure. ● Given an experimental task, select the appropriate plating method. PMID:22617405

  15. Alternative Method for the Mass Rearing of Lutzomyia (Lutzomyia) cruzi (Diptera: Psychodidae) in a Laboratory Setting.

    PubMed

    Oliveira, E F; Fernandes, W S; Oshiro, E T; Oliveira, A G; Galati, E A B

    2015-09-01

    The understanding of the transmission dynamics of Leishmania spp. Ross as well as the epidemiology and spread of leishmaniasis is related to parasite-vector-host interactions. These interactions can be studied using specimens of a sand fly population reared in the laboratory, exposing individuals to experimental infection for the investigation of vector competence and parameters of the vectorial capacity of the species. The present study sought to describe an alternative method for the implantation of a Lutzomyia (Lutzomyia) cruzi colony with wild specimens captured in the municipality of Corumbá, Brazil. With Method 1, engorged females were individualized for oviposition. The eggs were transferred to an acrylic petri dish with a layer of plaster on the bottom, on which food was placed after hatching of the first larvae. With Method 2, females were kept in groups for oviposition in containers, in which soil and food were placed on their bottom for the larvae. In addition, the exposure time of the larvae to light was reduced in comparison with Method 1. With Method 2, a significantly greater number of specimens of Lu. cruzi was obtained. The ratio between the number of emerged adults and the females followed for oviposition was 0.42 with Method 1 and 2.75 with Method 2. The optimization of the rearing conditions for Lu. cruzi will enable the establishment of a colony providing a sufficient number of specimens to develop experimental infection by Leishmania as well as vectorial competence and some parameters of the vectorial capacity of this sand fly. PMID:26336242

  16. Effect of learning on the oviposition preference of field-collected and laboratory-reared Chilo partellus (Lepidoptera: Crambidae) populations.

    PubMed

    Glas, J J; van den Berg, J; Potting, R P J

    2007-08-01

    Recent studies show that Vetiver grass, (Vetiveria zizanioides (L.) Nash), may have potential as a dead-end trap crop in an overall habitat management strategy for the spotted stem borer, Chilo partellus (Swinhoe) (Lepidoptera: Crambidae). Vetiver grass is highly preferred for oviposition, in spite of the fact that larval survival is extremely low on this grass. The oviposition behaviour of female Chilo partellus moths was investigated by determining the amount and size of egg batches allocated to maize and Vetiver plants and studying the effect of rearing conditions and oviposition experience on host plant selection. Two-choice preference tests were used to examine the effect of experience of maize (a suitable host plant) and Vetiver plants on the oviposition choice of C. partellus. For both field-collected and laboratory-reared moths, no significant differences were found in the preference distributions between the experienced groups. It is concluded that females do not learn, i.e. that they do not change their preference for Vetiver grass after having experienced oviposition on either maize or this grass, which supports the idea that trap cropping could have potential as a control method for C. partellus. Differences observed between field-collected and laboratory-reared moths in the amount and size of egg batches laid on maize and Vetiver grass indicate that data obtained from experiments with laboratory-reared insects should be treated with caution. PMID:17645823

  17. Comparison of laboratory and field bioassays of lab-reared Cydia pomonella (Lepidoptera: Tortricidae) quality and field performance.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Maximum production and fitness of insect species that are mass-reared for biological control programs such as the sterile insect technique (SIT) have benefitted from the employment of quality control and quality management. With a growing interest in the use of SIT as a tactic for the suppression/e...

  18. Quality control tests of lab-reared Cydia pomonella and Cactoblastis cactorum field performance: Comparison of laboratory and field bioassays.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Research, operational, and commercial programs which rely on mass-reared insects of high quality and performance, need accurate methods for monitoring quality degradation during each step of production, handling and release. With continued interest in the use of the sterile insect technique (SIT) a...

  19. Laboratory Techniques for the Blind

    ERIC Educational Resources Information Center

    Tombaugh, Dorothy

    1972-01-01

    Describes modifications of laboratory procedures for the BSCS Green Version biology, including dissection, microbiology, animal behavior, physiology, biochemistry, and genetics that make the methods suitable for direct experimentation by blind students. Discusses models as substitutes for microscopy. (AL)

  20. Induction of atrazine tolerance in a natural soil assemblage of microalgae reared in the laboratory.

    PubMed

    García-Villada, Libertad; Reboud, Xavier

    2007-01-01

    We investigated the occurrence of tolerance to atrazine (2-chloro-4-ethylamino-6-isopropylamino-s-triazine) in soil microalgae by means of the pollution-induced community tolerance methodology. To this end, a natural soil assemblage of microalgae, reared under laboratory conditions, was used as experimental model. Experimental cultures were exposed to 0.46, 0.93, 1.85, 2.70, and 5.40 mg atrazine/L medium for 40 days. After this chronic exposure, both untreated and atrazine-pretreated cultures were subjected to a short-term dose-effect study with atrazine using average growth rate as endpoint. Results showed that chronic exposure to atrazine induced significant changes at the community level, increasing its atrazine tolerance. In addition, atrazine-pretreated assemblages seemed to display, on average, a reduced growth rate compared with untreated assemblages in the absence of atrazine. Response to the presence of atrazine could thus be achieved with some fitness cost. This suggests that the soil microalgal productivity could be diminished after exposure to atrazine. PMID:16249030

  1. Genetic and environmental factors associated with laboratory rearing affect survival and assortative mating but not overall mating success in Anopheles gambiae sensu stricto.

    PubMed

    Paton, Doug; Touré, Mahamoudou; Sacko, Adama; Coulibaly, Mamadou B; Traoré, Sékou F; Tripet, Frédéric

    2013-01-01

    Anopheles gambiae sensu stricto, the main vector of malaria in Africa, is characterized by its vast geographical range and complex population structure. Assortative mating amongst the reproductively isolated cryptic forms that co-occur in many areas poses unique challenges for programs aiming to decrease malaria incidence via the release of sterile or genetically-modified mosquitoes. Importantly, whether laboratory-rearing affects the ability of An. gambiae individuals of a given cryptic taxa to successfully mate with individuals of their own form in field conditions is still unknown and yet crucial for mosquito-releases. Here, the independent effects of genetic and environmental factors associated with laboratory rearing on male and female survival, mating success and assortative mating were evaluated in the Mopti form of An. gambiae over 2010 and 2011. In semi-field enclosures experiments and despite strong variation between years, the overall survival and mating success of male and female progeny from a laboratory strain was not found to be significantly lower than those of the progeny of field females from the same population. Adult progeny from field-caught females reared at the larval stage in the laboratory and from laboratory females reared outdoors exhibited a significant decrease in survival but not in mating success. Importantly, laboratory individuals reared as larvae indoors were unable to mate assortatively as adults, whilst field progeny reared either outdoors or in the laboratory, as well as laboratory progeny reared outdoors all mated significantly assortatively. These results highlight the importance of genetic and environment interactions for the development of An. gambiae's full mating behavioral repertoire and the challenges this creates for mosquito rearing and release-based control strategies. PMID:24391719

  2. Investigation of Age Polyethism in Food Processing of the Fungus-Growing Termite Odontotermes formosanus (Blattodea: Termitidae) Using a Laboratory Artificial Rearing System.

    PubMed

    Li, Hongjie; Yang, Mengyi; Chen, Yonger; Zhu, Na; Lee, Chow-Yang; Wei, Ji-Qian; Mo, Jianchu

    2015-02-01

    Laboratory rearing systems are useful models for studying Rhinotermitid behavior. Information on the biology of fungus-growing termites, however, is limited because of the difficulty of rearing colonies in the laboratory settings. The physical structure of termite nests makes it impossible to photograph or to observe colonies in the field. In this study, an artificial rearing system for field-collected colonies of the fungus-growing termite Odontotermes formosanus (Shiraki) was developed to facilitate observation in the laboratory. We recorded colony activity within the artificial rearing system and documented a variety of social behaviors that occurred throughout the food processing of the colony. This complex miniature ecosystem was cooperatively organized via division of labor in the foraging and processing of plant materials, and the observed patterns largely resembled the caste and age-based principles present in Macrotermes colonies. This work extends our insights into polyethism in the subfamily Macrotermitinae. PMID:26470129

  3. Laboratory Rearing of Thitarodes armoricanus and Thitarodes jianchuanensis (Lepidoptera: Hepialidae), Hosts of the Chinese Medicinal Fungus Ophiocordyceps sinensis (Hypocreales: Ophiocordycipitaceae).

    PubMed

    Tao, Zui; Cao, Li; Zhang, Yi; Ye, Yunshou; Han, Richou

    2016-02-01

    Ophiocordyceps sinensis (Ophiocordycipitaceae) is an entomopathogenic fungus endemic to the Tibetan Plateau, at elevations ranging between 3,000 and 5,000 m. The fungus-insect complex is useful in healthcare but limited in the field, so there is an urgent need to develop an artificial rearing system of both the fungus and its insect hosts. Large-scale artificial rearing of the Thitarodes insect hosts is crucial. This paper reports results of the artificial cultivation and complete life tables of two host species of O. sinensis, Thitarodes armoricanus and Thitarodes jianchuanensis (Lepidoptera: Hepialidae), under low-altitude laboratory conditions. The larvae were reared on carrots in plastic containers at 9–13°C and 50–80% RH. Both experimental insect species had long and unusual life cycle; it took 263–494 and 443–780 d for T. jianchuanensis and T. armoricanus, respectively, to complete a developmental cycle, including egg, larval instars L1-L9, pupa, and adult. The larvae did develop into pupae from the L7, L8, or L9 instar larvae. Although the total survival rates of both insect species were low (12.0% for T. jianchuanensis and 1.6% for T. armoricanus), the experimental populations successfully developed into the next generation owing to high egg production by fertilized females (averages of 703 and 355 eggs per female in the Yunnan and Sichuan species, respectively). Successful artificial rearing of host insect species for O. sinensis under low temperature conditions will allow the cultivation of this important fungus-insect complex to ensure its protection as a bio-resource and for commercial supply. PMID:26567334

  4. Biology, life history, and laboratory rearing of Spathius galinae (Hymenoptera: Braconidae), a larval parasitoid of the invasive emerald ash borer (Coleoptera: Buprestidae).

    PubMed

    Duan, Jian J; Watt, Timothy J; Larson, Kristi

    2014-06-01

    Spathius galinae Belokobylskij & Strazanac is a recently described parasitoid of the emerald ash borer, Agrilus planipennis Fairmaire, in the Russian Far East, and is currently being considered for biocontrol introduction in the United States. Using A. planipennis larvae reared with freshly cut ash (Fraxinus spp.) sticks, we investigated the biology, life cycle, and rearing of S. galinae in the laboratory under normal rearing conditions (25 +/- 1 degrees C, 65 +/- 10% relative humidity, and a photoperiod of 16:8 [L:D] h). Our study showed that S. galinae took approximately 1 mo (29 d) to complete a single generation (from egg to adult) under the laboratory rearing conditions. After eclosion from eggs, larvae of S. galinae molted four times to reach the fifth instar, which then spun cocoons for pupation and development to adults. Adult female wasps had a median survival time of 7 wk with fecundity peaking 3 wk after emergence when reared in groups (of five females and five males) and 2 wk in single pairs. Throughout the life span, a single female S. galinae produced a mean (+/- SE) of 31 (+/- 3.0) progeny when reared in groups, and a mean (+/- SE) of 47 (+/- 5.3) progeny when reared in single pairs. Results from our study also showed that S. galinae could be effectively reared with A. planipennis larvae reared in both green (Fraxinus pennsylvanica Marshall) and tropical [Fraxinus uhdei (Wenzig) Lingelsh] ash sticks. However, the abortion (unemergence) rate of S. galinae progeny was much higher (20%) when reared with host larvae in green ash sticks than that (2.1%) in tropical ash sticks. PMID:25026651

  5. The Rearing and Biology of the Desert Beetle, Microdera punctipennis, Under Laboratory Conditions

    PubMed Central

    Wang, Yan; Liu, Xiaoning; Zhao, Jia; Rexili, Kelaimu; Ma, Ji

    2011-01-01

    Microdera punctipennis Kasz (Coleoptera: Tenebrionidae) is a unique species that lives in the desert region of Central Asia and has adopted a nocturnal habit to survive the desert environment. Female adults are larger in size than male adults. The female/male ratio was 1.04:1. A rearing method using reused plastic bottles was used. The rearing conditions were 30 ± 0.5°C, 30 ± 6% relative humidity (RH), and 16:8 L:D photoperiod. Cabbage was provided as food. Cannibalism was avoided by rearing one larva in a bottle. A complete life cycle was obtained under these conditions. The viability of eggs, larvae, prepupae, pupae, and teneral adults was 93.54%, 83.71%, 84.76%, 87.64%, and 93.59%, respectively. Embryogenesis took 7.35 days on average. The larval duration in each instar was 2.25 days. The mean duration of the larvae, prepupae, pupae, and teneral adult was 49.27, 7.05, 9.95, and 10.12 days, respectively. The coloration of each developmental stage gradually changed from creamy white to light brownish or black. Females commenced oviposition when their body color became black. On average, each female produced 568 eggs. PMID:21529250

  6. The rearing and biology of the desert beetle, Microdera punctipennis, under laboratory conditions.

    PubMed

    Wang, Yan; Liu, Xiaoning; Zhao, Jia; Rexili, Kelaimu; Ma, Ji

    2011-01-01

    Microdera punctipennis Kasz (Coleoptera: Tenebrionidae) is a unique species that lives in the desert region of Central Asia and has adopted a nocturnal habit to survive the desert environment. Female adults are larger in size than male adults. The female/male ratio was 1.04:1. A rearing method using reused plastic bottles was used. The rearing conditions were 30 ± 0.5 °C, 30 ± 6% relative humidity (RH), and 16:8 L:D photoperiod. Cabbage was provided as food. Cannibalism was avoided by rearing one larva in a bottle. A complete life cycle was obtained under these conditions. The viability of eggs, larvae, prepupae, pupae, and teneral adults was 93.54%, 83.71%, 84.76%, 87.64%, and 93.59%, respectively. Embryogenesis took 7.35 days on average. The larval duration in each instar was 2.25 days. The mean duration of the larvae, prepupae, pupae, and teneral adult was 49.27, 7.05, 9.95, and 10.12 days, respectively. The coloration of each developmental stage gradually changed from creamy white to light brownish or black. Females commenced oviposition when their body color became black. On average, each female produced 568 eggs. PMID:21529250

  7. MATERIALS AND TECHNIQUES FOR THE LANGUAGE LABORATORY.

    ERIC Educational Resources Information Center

    NAJAM, EDWARD W.

    THE PROCEEDINGS OF THE SECOND ANNUAL INDIANA-PURDUE LANGUAGE LABORATORY CONFERENCE ARE ORGANIZED, AFTER INTRODUCTORY STATEMENTS BY NAJAM AND LARSEN ON CONTEMPORARY TRENDS IN LANGUAGE INSTRUCTION, UNDER THREE GENERAL HEADINGS PLUS APPENDIXES. IN THE FIRST SECTION DEVOTED TO MATERIALS AND TECHNIQUES ARE ARTICLES BY HYER, GARIMALDI, EDDY, AND SMITH

  8. MATERIALS AND TECHNIQUES FOR THE LANGUAGE LABORATORY.

    ERIC Educational Resources Information Center

    NAJAM, EDWARD W.

    THE PROCEEDINGS OF THE SECOND ANNUAL INDIANA-PURDUE LANGUAGE LABORATORY CONFERENCE ARE ORGANIZED, AFTER INTRODUCTORY STATEMENTS BY NAJAM AND LARSEN ON CONTEMPORARY TRENDS IN LANGUAGE INSTRUCTION, UNDER THREE GENERAL HEADINGS PLUS APPENDIXES. IN THE FIRST SECTION DEVOTED TO MATERIALS AND TECHNIQUES ARE ARTICLES BY HYER, GARIMALDI, EDDY, AND SMITH…

  9. Behavior of steelhead fry in a laboratory stream is affected by fish density but not rearing environment

    USGS Publications Warehouse

    Riley, Stephen C.; Tatara, Christopher P.; Berejikian, Barry A.; Flagg, Thomas A.

    2009-01-01

    We quantified the aggression, feeding, dominance, position choice, and territory size of naturally reared steelhead Oncorhynchus mykiss fry stocked with two types of hatchery-reared fry (from conventional and enriched rearing environments) at two densities in experimental flumes to determine how rearing environment and fish density affect the behavior of steelhead fry. We found that fry density had a significant effect on most response variables but that rearing treatment did not. The rates of threats and attacks were positively correlated with fry density, but the overall feeding rate was negatively correlated. Naturally reared fry were dominant more often at low densities, and hatchery-reared fry were dominant more often at high densities. There were no significant effects of hatchery rearing treatment on aggression, feeding, dominance, or territory size. The only significant effect of rearing treatment was on the position of naturally reared fry, which occupied more upstream positions when stocked with conventional than with enriched hatchery-reared fry. Overall, rearing environment had relatively little influence on the behavior of steelhead fry. Our results indicate that stocking hatchery-reared steelhead fry at low densities may have effects on similar-size wild fish comparable to an equivalent increase in the density of wild fish. We suggest that releasing hatchery-reared steelhead fry as a supplementation strategy may have few direct negative ecological effects on wild fry.

  10. A laboratory technique to study the effects of Varroa destructor and viruses on developing worker honey bees

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Existing techniques for in vitro rearing honey bees and Varroa involve brood manipulation. In this laboratory study, we used larvae that were naturally developing in a comb as Varroa- and virus-inoculation hosts. In Trial 1, we used L4 larvae and newly sealed larvae (NSL) as hosts which were inocula...

  11. Graphing techniques for materials laboratory using Excel

    NASA Technical Reports Server (NTRS)

    Kundu, Nikhil K.

    1994-01-01

    Engineering technology curricula stress hands on training and laboratory practices in most of the technical courses. Laboratory reports should include analytical as well as graphical evaluation of experimental data. Experience shows that many students neither have the mathematical background nor the expertise for graphing. This paper briefly describes the procedure and data obtained from a number of experiments such as spring rate, stress concentration, endurance limit, and column buckling for a variety of materials. Then with a brief introduction to Microsoft Excel the author explains the techniques used for linear regression and logarithmic graphing.

  12. DAILY GROWTH OF THE JUVENILE FISH (MENIDIA MENIDIA) IN THE NATURAL HABITAT COMPARED WITH JUVENILES REARED IN THE LABORATORY

    EPA Science Inventory

    The Atlantic Silverside (Menidia menidia) is an abundant annual fish in Rhode Island, USA, waters and is being cultured in the Narragansett Laboratory of EPA for use in toxicological bioassays following culture techniques described by Beck (1977). It is desirable for laboratory-r...

  13. Using near-infrared spectroscopy to resolve the species, gender, age, and the presence of Wolbachia infection in laboratory-reared Drosophila

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The aim of the study was to determine the accuracy of near-infrared spectroscopy (NIRS) in determining species, gender, age and the presence of the common endosymbiont Wolbachia in laboratory reared Drosophila. NIRS measures absorption of light by organic molecules. Initially, a calibration model wa...

  14. Effects of ambient temperature on egg and larval development of the invasive emerald ash borer (Coleoptera: Buprestidae): implications for laboratory-rearing

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The emerald ash borer, Agrilus planipennis Fairmaire, an invasive beetle from Asia causing large scale ash (Fraxinus spp.) mortality in North America, has been extremely difficult to rear in the laboratory because of its long life cycle and cryptic nature of immature stages. This lack of effective ...

  15. Herpetogramma bipunctalis (Lepidoptera: Crambidae) biology and techniques for rearing on leaves of the blackberry (Rubus spp., Rosaceae).

    PubMed

    Diez-Rodríguez, G I; Hübner, L K; Antunes, L E C; Nava, D E

    2013-02-01

    The larvae of the southern beet webworm Herpetogramma bipunctalis (Fabricius, 1794) damage the leaves of species in the plant genus Rubus. The present study investigated the biology of H. bipunctalis and developed a protocol for raising H. bipunctalis under laboratory conditions. On the basis of the biological data, we devised a life table. In order to develop the rearing procedures, we determined which oviposition substrate and blackberry cultivar were the most appropriate for larval development. The mean durations of the egg, larval, and pupal stages were 5.59 days, 26.37 days, and 13.37 days, respectively, and the corresponding survival rates were 80.83%, 49.07%, and 83.23%. The mean pupal weight was 0.0491 g for males and 0.0536 g for females. The mean life cycle (egg-to-adult) period was 45.33 days, and overall survival to adulthood was 33.01%. H. bipunctalis females laid a mean of 252.63 eggs over a mean of 13.60 days of oviposition; the mean pre-oviposition period was 2.67 days. Mean female and male life spans were 17.51 and 19.25 days, respectively, and the sex ratio was 0.51. The life-table data indicated that H. bipunctalis can reproduce 57.9 times per generation. Each cage contained one blackberry leaf placed on a paper towel. This method allowed us to obtain the greatest number of eggs. The larval stage was shorter for insects reared on leaves of the Guarani cultivar than for those reared on leaves of the Xavante cultivar (22.63 vs. 26.37 days). These basic data can aid in establishing strategies for the management of H. bipunctalis on blackberry farms. PMID:23644800

  16. Tissue invasion of laboratory-reared Biomphalaria glabrata by a harpacticoid copepod.

    PubMed

    Sullivan, John T; Yeung, John T

    2011-06-01

    Copepods were observed in the tissues of 3 of 23 Biomphalaria glabrata snails examined histologically. All were heavily encapsulated by hemocytes and were dead. The copepods are most likely members of the order Harpacticoida, based on external morphology. This tissue invasion appears to be accidental rather than symbiotic or predatory, but could be a cause of observed mortality in laboratory snail colonies. PMID:21414323

  17. Caribbean fruit fly, Anastrepha suspensa (Diptera:Tephritidae): Life history and laboratory rearing methods.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Caribbean fruit fly, Anastrepha suspensa (Loew), is and agricultural pest established throughout south Florida, where it poses a threat to commercial citrus, guava, and other tropical and subtropical fruit crops. This poster outlines the protocols used at the USDA-ARS laboratory in Miami, FL, fo...

  18. INNOVATIONS IN EQUIPMENT AND TECHNIQUES FOR THE BIOLOGY TEACHING LABORATORY.

    ERIC Educational Resources Information Center

    BARTHELEMY, RICHARD E.; AND OTHERS

    LABORATORY TECHNIQUES AND EQUIPMENT APPROPRIATE FOR TEACHING BIOLOGICAL SCIENCE CURRICULUM STUDY BIOLOGY ARE EMPHASIZED. MAJOR CATEGORIES INCLUDE (1) LABORATORY FACILITIES, (2) EQUIPMENT AND TECHNIQUES FOR CULTURE OF MICRO-ORGANISMS, (3) LABORATORY ANIMALS AND THEIR HOUSING, (4) TECHNIQUES FOR STUDYING PLANT GROWTH, (5) TECHNIQUES FOR STUDYING…

  19. SNPfisher: tools for probing genetic variation in laboratory-reared zebrafish.

    PubMed

    Butler, Matthew G; Iben, James R; Marsden, Kurt C; Epstein, Jonathan A; Granato, Michael; Weinstein, Brant M

    2015-04-15

    Single nucleotide polymorphisms (SNPs) are the benchmark molecular markers for modern genomics. Until recently, relatively few SNPs were known in the zebrafish genome. The use of next-generation sequencing for the positional cloning of zebrafish mutations has increased the number of known SNP positions dramatically. Still, the identified SNP variants remain under-utilized, owing to scant annotation of strain specificity and allele frequency. To address these limitations, we surveyed SNP variation in three common laboratory zebrafish strains using whole-genome sequencing. This survey identified an average of 5.04 million SNPs per strain compared with the Zv9 reference genome sequence. By comparing the three strains, 2.7 million variants were found to be strain specific, whereas the remaining variants were shared among all (2.3 million) or some of the strains. We also demonstrate the broad usefulness of our identified variants by validating most in independent populations of the same laboratory strains. We have made all of the identified SNPs accessible through 'SNPfisher', a searchable online database (snpfisher.nichd.nih.gov). The SNPfisher website includes the SNPfisher Variant Reporter tool, which provides the genomic position, alternate allele read frequency, strain specificity, restriction enzyme recognition site changes and flanking primers for all SNPs and Indels in a user-defined gene or region of the zebrafish genome. The SNPfisher site also contains links to display our SNP data in the UCSC genome browser. The SNPfisher tools will facilitate the use of SNP variation in zebrafish research as well as vertebrate genome evolution. PMID:25813542

  20. Quality of mass-reared codling moth (Lepidoptera: Tortricidae) after long distance transportation 1. Logistics of shipping procedures and quality parameters as measured in the laboratory.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The sterile insect technique is a proven effective control tactic against lepidopteran pests when applied in an area-wide integrated pest management programme. The construction of insect mass-rearing facilities requires considerable investment and moth control strategies that include the use of ster...

  1. Dispersal and oviposition of laboratory-reared gravid females of Toxorhynchites moctezuma in an arid urban area of Sonora, Mexico.

    PubMed

    Macías-Duarte, Alberto; Alvarado-Castro, J Andrés; Dórame-Navarro, María E; Félix-Torres, A Amalia

    2009-12-01

    Dengue is a serious public health problem worldwide. Biological control of its vector, Aedes aegypti, remains a feasible option in light of increasing urbanization and insecticide resistance. We studied the dispersal and oviposition activity of Toxorhynchites moctezuma in a dengue-endemic urban area in SSonora, Mexico, to provide information about the potential of Toxorhynchites as a control agent for Ae. aegypti in arid areas. We released 210 and 100 laboratory-reared gravid females of Tx. moctezuma in 2 city blocks during the summer and fall of 1993. We set 3 1-liter containers and 1 car tire as sentinel traps at each of 10 backyards within each city block. Spatial and temporal patterns of dispersal and oviposition activity differed between city blocks and between releases. However, a Cox regression analysis showed no significant difference in the per-day probability of Tx. moctezuma oviposition events in sentinel traps between summer and fall releases. Per-day oviposition probability was nearly 5 times greater for sentineltraps that contained larvae of Ae. aegypti, suggesting a high specificity of the predator for its prey. The proportion of sentinel traps positive for Tx. moctezuma eggs did not increase substantially after the 8th day piost-release, reaching 66% and 23% for sentinel traps with and without Ae. aegypti larvae, respectively. PMID:20099587

  2. Using Near-Infrared Spectroscopy to Resolve the Species, Gender, Age, and the Presence of Wolbachia Infection in Laboratory-Reared Drosophila

    PubMed Central

    Aw, Wen C.; Dowell, Floyd E.; Ballard, J. William O.

    2012-01-01

    The aim of the study was to determine the accuracy of near-infrared spectroscopy (NIRS) in determining species, gender, age, and the presence of the common endosymbiont Wolbachia in laboratory-reared Drosophila. NIRS measures the absorption of light by organic molecules. Initially, a calibration model was developed for each study. An independent set with flies not involved in initial cross-validation was then used to validate the accuracy of each calibration model. Flies from the independent sets were correctly classified into Drosophila melanogaster and Drosophila simulans with 94% and 82% accuracy, respectively, whereas flies were successfully classified by gender with accuracy greater than 90%. In the age grading test, correlation plots of the actual and predicted age for males and females of D. melanogaster and D. simulans were shown to be overlapping between the adjacent age groups. It is, however, possible to predict the age of flies as less than 9 days of age with 62–88% accuracy and flies that are equal to or older than 9 days of age with 91–98% accuracy. Finally, we used NIRS to detect the presence of Wolbachia in flies. Flies from the independent sets were successfully identified as infected or not infected with Wolbachia with approximately 90% accuracy. These results suggest that NIRS has the potential to quantify the species, gender, and presence of Wolbachia in fly populations. However, additional optimization of the protocol may be necessary before the technique can reliably estimate fly age. PMID:22973543

  3. Effects of ambient temperature on egg and larval development of the invasive emerald ash borer (Coleoptera: Buprestidae): implications for laboratory rearing.

    PubMed

    Duan, Jian J; Watt, Tim; Taylor, Phil; Larson, Kristi; Lelito, Jonathan P

    2013-10-01

    The emerald ash borer, Agrilus planipennis Fairmaire, an invasive beetle from Asia causing large scale ash (Fraxinus) mortality in North America, has been extremely difficult to rear in the laboratory because of its long life cycle and cryptic nature of immature stages. This lack of effective laboratory-rearing methods has not only hindered research into its biology and ecology, but also mass production of natural enemies for biological control of this invasive pest. Using sticks from the alternate host plant, Fraxinus uhdei (Wenzig) Lingelsh, we characterized the stage-specific development time and growth rate of both emerald ash borer eggs and larvae at different constant temperatures (12-35 degrees C) for the purpose of developing effective laboratory-rearing methods. Results from our study showed that the median time for egg hatching decreased from 20 d at 20 degrees C to 7 d at 35 degrees C, while no emerald ash borer eggs hatched at 12 degrees C. The developmental time for 50% of emerald ash borer larvae advancing to third, fourth, and J-larval stages at 20 degrees C were 8.3, 9.1, and 12.3 wk, respectively, approximately two times longer than at 30 degrees C for the corresponding instars or stages. In contrast to 30 degrees C, however, the development times of emerald ash borer larvae advancing to later instars (from oviposition) were significantly increased at 35 degrees C, indicating adverse effects of this high temperature. The optimal range of ambient temperature to rear emerald ash borer larvae should be between 25-30 degrees C; however, faster rate of egg and larval development should be expected as temperature increases within this range. PMID:24224252

  4. Laboratory Testing of Volcanic Gas Sampling Techniques

    NASA Astrophysics Data System (ADS)

    Kress, V. C.; Green, R.; Ortiz, M.; Delmelle, P.; Fischer, T.

    2003-12-01

    A series of laboratory experiments were performed designed to calibrate several commonly used methods for field measurement of volcanic gas composition. H2, CO2, SO2 and CHCl2F gases were mixed through carefully calibrated rotameters to form mixtures representative of the types of volcanic compositions encountered at Kilauea and Showa-Shinzan. Gas mixtures were passed through a horizontal furnace at 700oC to break down CHCl2F and form an equilibrium high-temperature mixture. With the exception of Giggenbach bottle samples, all gas sampling was performed adjacent to the furnace exit in order to roughly simulate the air-contaminated samples encountered in Nature. Giggenbach bottle samples were taken from just beyond the hot-spot 10cm down the furnace tube to minimize atmospheric contamination. Alkali-trap measurements were performed by passing gases over or bubbling gases through 6N KOH, NaOH or LiOH solution for 10 minutes. Results were highly variable with errors in measured S/Cl varying from +1600% to -19%. In general reduced Kilauea compositions showed smaller errors than the more oxidized Showa-Shinzan compositions. Results were not resolvably different in experiments where gas was bubbled through the alkaline solution. In a second set of experiments, 25mm circles of Whatman 42 filter paper were impregnated with NaHCO3or KHCO3 alkaline solutions stabilized with glycerol. Some filters also included Alizarin (5.6-7.2) and neutral red (6.8-8.0) Ph indicator to provide a visual monitor of gas absorption. Filters were mounted in individual holders and used in stacks of 3. Durations were adjusted to maximize reaction in the first filter in the stack and minimize reaction in the final filter. Errors in filter pack measurements were smaller and more systematic than the alkali trap measurements. S/Cl was overestimated in oxidized gas mixtures and underestimated in reduced mixtures. Alkali-trap methods allow extended unattended monitoring of volcanic gasses, but our results suggest that they are poor recorders of gas composition. Filter pack methods are somewhat better, but are more difficult to interpret than previously recognized. We suggest several refinements to the filter-pack technique that can improve accuracy. Giggenbach bottles remain the best method for volcanic gas sampling, despite the inherent difficulty and danger of obtaining samples in active volcanic environments. Relative merits of different alkali solutions and indicators are discussed.

  5. Biological and genetic characteristics of Glyptotendipes tokunagai (Diptera: Chironomidae) on the basis of successive rearing of forty-two generations over seven years under laboratory conditions.

    PubMed

    Baek, Min Jeong; Yoon, Tae Joong; Kang, Hyo Jeong; Bae, Yeon Jae

    2014-10-01

    Members of the nonbiting midge family Chironomidae have been used worldwide as water-quality indicators or toxicity test organisms. The purpose of this study was to establish the chironomid Glyptotendipes tokunagai Sasa as a new test species by conducting successive rearing under laboratory conditions. We monitored biological and genetic aspects of >42 successive generations over 7 yr, and also compared the development of the 39th generation with the fourth generation under five constant temperatures of 15, 20, 25, 30, and 35C. We observed that the number of eggs in an egg mass and the adult body sizes decreased rapidly in the early generations, and thereafter tended to stabilize from the fifth generation to the 42nd generation. In all generations, the mean hatching rate was >75%. Males were predominant in the early generations, but the sex ratio increased to 0.5 (ranged 0.24-0.61) in later generations. The genetic divergence of the reared generations, analyzed by using the mitochondrial cytochrome c oxidase subunit I gene, decreased from 0.0049 to 0.0004 as the generations progressed. In comparison with the fourth generation, the mortality and developmental time of the 39th generation were generally greater, and the adult body sizes were generally smaller. The estimated low developmental threshold temperatures of eggs, male larvae to male adults, and female larvae to female adults were 9.6, 11.3, and 9.7C, respectively. The optimal rearing temperature was determined to be 25C. This is the first record of domesticated rearing of a wild chironomid species under laboratory conditions for >7 yr. PMID:25203599

  6. The North American strain of viral hemorrhagic septicemia virus is highly pathogenic for laboratory-reared Pacific herring (Clupea pallasi)

    USGS Publications Warehouse

    Kocan, R.; Bradley, M.; Elder, N.; Meyers, T.; Batts, W.; Winton, J.

    1997-01-01

    Specific-pathogen-free Pacific herring Clupea pallasi were reared in the laboratory from eggs and then challenged at 5, 9, and 13 months of age by waterborne exposure to low (101.5–2.5 plaque-forming units [PFU] per milliliter), medium (103.5–4.5 PFU/mL), or high (105.5–6.5 PFU/mL) levels of a North American isolate of viral hemorrhagic septicemia virus (VHSV). The fish were extremely susceptible to the virus, showing clinical disease, mortality approaching 100%, and only a limited increase in resistance with age. Mortality began 4–6 d after exposure and peaked at approximately day 7 in fish exposed to high levels of virus. Whereas the mean time to death showed a significant dose response (P < 0.001), the percent mortality and virus titers in dead fish were generally high in all groups regardless of initial challenge dose. External signs of disease were usually limited to 1–2-mm hemorrhagic areas on the lower jaw and isthmus and around the eye, but 2 of 130 infected fish exhibited extensive cutaneous hemorrhaging. Histopathologic examination of tissues from moribund fish sampled at 2–8 d after exposure revealed multifocal coagulative necrosis of hepatocytes, diffuse necrosis of interstitial hematopoietic tissues in the kidney, diffuse necrosis of the spleen, epidermis, and subcutis, and occasional necrosis of pancreatic acinar cells. Virus titers in tissues of experimentally infected herring were first detected 48 h after exposure and peaked 6-8 d after exposure at 107.7 PFU/g. Fish began shedding virus at 48 h after exposure with titers in the flow-through aquaria reaching 102.5 PFU/mL at 4–5 d after exposure, just before peak mortality. When the water flow was turned off for 3 h, titers in the water rose to 103.5 PFU/mL, and the amount of virus shed by infected fish (on average, greater than 106.5 PFU/h per fish) appeared sufficient to sustain a natural epizootic among schooling herring. Taken together, these data suggest that VHSV could be a significant limiting factor for populations of Pacific herring.

  7. Techniques and Strategies in Clinical Laboratory Science Education.

    ERIC Educational Resources Information Center

    Vittetoe, Marie; Morris, Frances J.

    A mailed survey sent to faculty from 132 medical technology schools, 64 medical laboratory technician schools, and 58 certified laboratory assistant schools yielded 355 responses concerning most frequently vs. least frequently used teaching techniques and most effective vs. least effective teaching techniques. Information on where and how…

  8. Validation of a New Larval Rearing Unit for Aedes albopictus (Diptera: Culicidae) Mass Rearing

    PubMed Central

    Gilles, Jérémie R. L.; Bellini, Romeo

    2014-01-01

    The mosquito larval rearing unit developed at the Insect Pest Control Laboratory (IPCL) of the FAO/IAEA Joint Division was evaluated for its potential use for Aedes albopictus (Skuse, 1895) mass rearing in support of the development of a sterile insect technique (SIT) package for this species. The use of the mass rearing trays and rack did not adversely affect larval development, pupation and survival rates and allowed the management of large larval rearing colonies with reduced space requirements in comparison with classical individual trays. The effects of larval density, water temperature and diet composition on pupal production and size differentiation for sex separation efficacy were analyzed for individual mass rearing trays as well as multiple trays stacked within the dedicated rack unit. Best results were obtained using eighteen thousand larvae per tray at a density of 3 larvae per ml of deionized water at a temperature of 28°C on a diet consisting of 50% tuna meal, 36% bovine liver powder, 14% brewer's yeast and, as an additive, 0.2 gr of Vitamin Mix per 100 ml of diet solution. Pupae were harvested on the sixth day from larval introduction at L1 stage and males were separated out by the use of a 1400 µm sieve with 99.0% accuracy with a recovery rate of ca. 25% of the total available males. With the use of this larval rearing unit, an average production of 100,000 male pupae per week can be achieved in just 2 square meter of laboratory space. Compared to previous laboratory rearing method, the same pupal production and sex separation efficacy could only be achieved by use of ca. 200 plastic trays which required the space of two 5 square meter climatic-controlled rooms. PMID:24647347

  9. Laboratory reptile surgery: principles and techniques.

    PubMed

    Alworth, Leanne C; Hernandez, Sonia M; Divers, Stephen J

    2011-01-01

    Reptiles used for research and instruction may require surgical procedures, including biopsy, coelomic device implantation, ovariectomy, orchidectomy, and esophogostomy tube placement, to accomplish research goals. Providing veterinary care for unanticipated clinical problems may require surgical techniques such as amputation, bone or shell fracture repair, and coeliotomy. Although many principles of surgery are common between mammals and reptiles, important differences in anatomy and physiology exist. Veterinarians who provide care for these species should be aware of these differences. Most reptiles undergoing surgery are small and require specific instrumentation and positioning. In addition, because of the wide variety of unique physiologic and anatomic characteristics among snakes, chelonians, and lizards, different techniques may be necessary for different reptiles. This overview describes many common reptile surgery techniques and their application for research purposes or to provide medical care to research subjects. PMID:21333158

  10. Laboratory Reptile Surgery: Principles and Techniques

    PubMed Central

    Alworth, Leanne C; Hernandez, Sonia M; Divers, Stephen J

    2011-01-01

    Reptiles used for research and instruction may require surgical procedures, including biopsy, coelomic device implantation, ovariectomy, orchidectomy, and esophogostomy tube placement, to accomplish research goals. Providing veterinary care for unanticipated clinical problems may require surgical techniques such as amputation, bone or shell fracture repair, and coeliotomy. Although many principles of surgery are common between mammals and reptiles, important differences in anatomy and physiology exist. Veterinarians who provide care for these species should be aware of these differences. Most reptiles undergoing surgery are small and require specific instrumentation and positioning. In addition, because of the wide variety of unique physiologic and anatomic characteristics among snakes, chelonians, and lizards, different techniques may be necessary for different reptiles. This overview describes many common reptile surgery techniques and their application for research purposes or to provide medical care to research subjects. PMID:21333158

  11. Ultrastructure and distribution of intracellular rickettsia-like microorganisms in various organs of the laboratory-reared adult tick Argas (Persicargas) arboreus (Ixodoidea: Argasidae).

    PubMed

    el Shoura, S M

    1990-08-01

    Rod (RS) and coccoid (CS) rickettsia-like microorganisms were found in single and group forms in organs of the laboratory-reared adult ticks Argas (Persicarges) arboreus. RS are distributed in most organs but are mainly concentrated in the salivary glands, mid-gut, and testes. CS single forms were concentrated in the rectal sac, while the group forms were limited to Malpighian tubules and haemocytes of both sexes. The primary oocytes were heavily infected with both forms of CS. No RS or CS were detected in the muscles. Despite the structural differences between RS and CS, they are suggested to be different morphotypes of the same organism. PMID:2226072

  12. Thermal dependency of shell growth, microstructure, and stable isotopes in laboratory-reared Scapharca broughtonii (Mollusca: Bivalvia)

    NASA Astrophysics Data System (ADS)

    Nishida, Kozue; Suzuki, Atsushi; Isono, Ryosuke; Hayashi, Masahiro; Watanabe, Yusuke; Yamamoto, Yuzo; Irie, Takahiro; Nojiri, Yukihiro; Mori, Chiharu; Sato, Mizuho; Sato, Kei; Sasaki, Takenori

    2015-07-01

    We experimentally examined the growth, microstructure, and chemistry of shells of the bloody clam, Scapharca broughtonii (Mollusca: Bivalvia), reared at five temperatures (13, 17, 21, 25, and 29°C) with a constant pCO2 condition (˜450 μatm). In this species, the exterior side of the shell is characterized by a composite prismatic structure; on the interior side, it has a crossed lamellar structure on the interior surface. We previously found a negative correlation between temperature and the relative thickness of the composite prismatic structure in field-collected specimens. In the reared specimens, the relationship curve between temperature and the growth increment of the composite prismatic structure was humped shaped, with a maximum at 17°C, which was compatible with the results obtained in the field-collected specimens. In contrast, the thickness of the crossed lamellar structure was constant over the temperature range tested. These results suggest that the composite prismatic structure principally accounts for the thermal dependency of shell growth, and this inference was supported by the finding that shell growth rates were significantly correlated with the thickness of the composite prismatic structure. We also found a negative relationship between the rearing temperature and δ18O of the shell margin, in close quantitative agreement with previous reports. The findings presented here will contribute to the improved age determination of fossil and recent clams based on seasonal microstructural records.

  13. Rearing insects on artificial diets

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Insects are reared in the laboratory for various purposes. They may be reared either on their natural food or artificial diets. Developing artificial diets may be difficult and time consuming but once optimized, artificial diets usually are simple to prepare and easy to use. Because they are process...

  14. Laboratory Diagnostic Techniques for Entamoeba Species

    PubMed Central

    Fotedar, R.; Stark, D.; Beebe, N.; Marriott, D.; Ellis, J.; Harkness, J.

    2007-01-01

    The genus Entamoeba contains many species, six of which (Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, Entamoeba polecki, Entamoeba coli, and Entamoeba hartmanni) reside in the human intestinal lumen. Entamoeba histolytica is the causative agent of amebiasis and is considered a leading parasitic cause of death worldwide in humans. Although recent studies highlight the recovery of E. dispar and E. moshkovskii from patients with gastrointestinal symptoms, there is still no convincing evidence of a causal link between the presence of these two species and the symptoms of the host. New approaches to the identification of E. histolytica are based on detection of E. histolytica-specific antigen and DNA in stool and other clinical samples. Several molecular diagnostic tests, including conventional and real-time PCR, have been developed for the detection and differentiation of E. histolytica, E. dispar, and E. moshkovskii in clinical samples. The purpose of this review is to discuss different methods that exist for the identification of E. histolytica, E. dispar, and E. moshkovskii which are available to the clinical diagnostic laboratory. To address the need for a specific diagnostic test for amebiasis, a substantial amount of work has been carried out over the last decade in different parts of the world. The molecular diagnostic tests are increasingly being used for both clinical and research purposes. In order to minimize undue treatment of individuals infected with other species of Entamoeba such as E. dispar and E. moshkovskii, efforts have been made for specific diagnosis of E. histolytica infection and not to treat based simply on the microscopic examination of Entamoeba species in the stool. The incorporation of many new technologies into the diagnostic laboratory will lead to a better understanding of the public health problem and measures to control the disease. PMID:17630338

  15. Nature beats nurture: a case study of the physiological fitness of free-living and laboratory-reared male Anopheles gambiae s.l.

    PubMed

    Huho, B J; Ng'habi, K R; Killeen, G F; Nkwengulila, G; Knols, B G J; Ferguson, H M

    2007-08-01

    Laboratory experimentation forms the basis for most of our knowledge of the biology of many organisms, in particular insects. However, the accuracy with which laboratory-derived estimates of insect life history and behaviour can predict their fitness and population dynamics in the wild is rarely validated. Such comparison is especially important in cases where laboratory-derived information is used to formulate and implement strategies for the genetic control of insects in nature. We have conducted a comparative study of the reproductive potential and life history of male Anopheles gambiae Gilies sensu lato mosquitoes from both standardized laboratory conditions and from natural field settings. We measured three indirect indicators of male mosquito fitness: energetic reserves, body size and survival, in a bid to determine whether the demographics and energetic limitations of wild males can be correctly predicted from their laboratory counterparts. Crucially, the body size and lipid reserves of wild males were substantially greater than those reared under standard laboratory conditions. We caution that the energetic limitations of insects as identified in the laboratory may underestimate their resilience in the wild, and discuss the implications of this phenomenon with respect to vector-borne disease control programmes based on genetic control of mosquitoes. PMID:17690243

  16. Bacterial communities associated with the digestive tract of the predatory ground beetle, Poecilus chalcites, and their response to laboratory rearing and antibiotic treatment

    SciTech Connect

    Michael Lehman

    2008-06-01

    Ground beetles such as Poecilus chalcites (Coleoptera: Carabidae) are beneficial insects in agricultural systems where they contribute to the control of insect and weed pests. We assessed the complexity of bacterial communities occurring in the digestive tracts of field-collected P. chalcites using terminal restriction fragment length polymorphism analyses of polymerase chain reaction-amplified 16S rRNA genes. Bacterial identification was performed by the construction of 16S rRNA gene clone libraries and sequence analysis. Intestinal bacteria in field-collected beetles were then compared to those from groups of beetles that were reared in the lab on an artificial diet with and without antibiotics. Direct cell counts estimated 1.5 × 10S bacteria per milliliter of gut. The digestive tract of field-collected P. chalcites produced an average of 4.8 terminal restriction fragments (tRF) for each beetle. The most abundant clones were affiliated with the genus Lactobacillus, followed by the taxa Enterobacteriaceae, Clostridia, and Bacteriodetes. The majority of the sequences recovered were closely related to those reported from other insect gastrointestinal tracts. Lab-reared beetles produced fewer tRF, an average of 3.1 per beetle, and a reduced number of taxa with a higher number of clones from the family Enterobacteriaceae compared to the field-collected beetles. Antibiotic treatment significantly (p < 0.05) reduced the number of tRF per beetle and selected for a less diverse set of bacterial taxa. We conclude that the digestive tract of P. chalcites is colonized by a simple community of bacteria that possess autochthonous characteristics. Laboratory-reared beetles harbored the most common bacteria found in field-collected beetles, and these bacterial communities may be manipulated in the laboratory with the addition of antibiotics to the diet to allow study of functional roles.

  17. Laboratory testing of closure cap repair techniques

    SciTech Connect

    Persoff, P.; Moridis, G.; Tuck, D.M.

    1996-10-01

    Landfill design requires a low permeability closure cap as well as a low permeability liner. The Savannah River Site, in South Carolina, has approximately 85 acres of mixed waste landfills covered with compacted kaolin clay. Maintaining low permeability of the clay cap requires both that the permeability of the compacted clay itself remain low and that the integrity of the barrier be maintained. Barrier breaches typically result from penetration by roots or animals, and especially cracks caused by uneven settling or desiccation. In this study, clay layers, 0.81 m in diameter and 7.6 cm thick, were compacted in 7 lysimeters to simulate closure caps. The hydraulic conductivity of each layer was measured, and the compacted clay layers (CCL`s) were cracked by drying. Then various repair techniques were applied and the effectiveness of each repair was assessed by remeasuring the hydraulic conductivity. Finally the repaired CCL was again dried and measured to determine how the repair responded to the conditions that caused the original failure. For a full report of this investigation see Persoff et al. Six repair techniques have been tested, four of which involve the use of injectable barrier liquids colloidal silica (CS) and polysiloxane (PSX) described below: (I) covering the crack with a bentonite geosynthetic clay liner (GCL), (ii) recompaction of new kaolinite at STD+3 moisture content joined to existing kaolinite that had dried and shrunk, (iii) direct injection of colloidal silica to a crack, (iv) injection of colloidal silica (CS) to wells in an overlying sand layer, (v) direct injection of polysiloxane to a crack, and (vi), injection of polysiloxane (PSX) to wells in an overlying soil layer.

  18. Costly nutritious diets do not necessarily translate into better performance of artificaially reared fruit files (Diptera: Tephritidae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Protein, lipid, carbohydrate and energy contents of three artificial diets (Xal2, Met1 and Met2) used for laboratory-rearing and mass-rearing the Mexican fruit fly, Anastrepha ludens (Loew), for a sterile insect technique (SIT) program were measured. The larval survival, pupation, pupal weight, adu...

  19. Tick rearing and in vitro feeding

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The production of high quality laboratory-reared ticks is essential to many studies on tick biology, control and interactions with pathogenic agents. Tick rearing is complicated by the requirement of blood feeding multiple times throughout the life cycle and time- intensive rearing procedures (Gregs...

  20. Growth patterns, chemical composition and oxygen consumption in early juvenile Hyas araneus (Decapoda: Majidae) reared in the laboratory

    NASA Astrophysics Data System (ADS)

    Anger, K.; Harms, J.; Christiansen, M. E.; Süsens, U.; Wilmes, B.

    1992-03-01

    Early (instar I and II) juveniles of the spider crab Hyas araneus were reared under constant conditions (12 °C, 32‰S) in the laboratory, and their growth, biochemical composition, and respiration were studied. Every second day, dry weight (W), ash-free dry weight (AFW), and contents of ash, organic and inorganic carbon (C), nitrogen (N), hydrogen (H), protein, chitin, lipid, and carbohydrates were measured, as well as oxygen consumption. Changes in the absolute amounts of W. AFW, and C, N, and H during the moulting cycle are described with various regression equations as functions of age within a given instar. These patterns of growth differ in part from those that have been observed during previous studies in larval stages of the same and some other decapod species, possibly indicating different growth strategies in larvae and juveniles. There were clear periodic changes in ash (% of W) and inorganic C (as % of total C), with initially very low and then steeply increasing values in postmoult, a maximum in intermoult, and decreasing figures during the premoult phase of each moulting cycle. Similar patterns were observed in the chitin fraction, reaching a maximum of 16% of W (31% of AFW). Ash, inorganic C, and chitin represent the major components of the exoskeleton and hence, changes in their amounts are associated with the formation and loss of cuticle material. Consequently, a high percentage of mineral matter was lost with the exuvia (76% of the late premoult [LPM] ash content, 74% of inorganic C), but relatively small fractions of LPM organic matter (15% of AFW, 11% of organic C, 5 6% of N and H). These cyclic changes in the cuticle caused an inverse pattern of variation in the percentage values (% of W) of AFW, organic C, N, H, and biochemical constituents other than chitin. When these measures of living biomass were related to, exclusively, the organic body fraction (AFM), much less variation was found during individual moulting cycles, with values of about 43 52% in organic C, 9 10% in N, 6 9% H, 31 49% of AFW in protein, 3 10% in lipid, and <1% in carbohydrates. All these constituents showed, on the average, a decreasing tendency during the first two crab instars, whereas N remained fairly constant. It cannot be explained at present, what other elements and biochemical compounds, respectively, might replace these decreasing components of AFW. Decreasing tendencies during juvenile growth were observed also in the organic C/N and in the lipid/protein weight ratios, both indicating that the proportion of lipid decreased at a higher rate than that of protein. Changes were observed also in the composition of inorganic matter, with significantly lower inorganic C in early postmoult (2 4% of ash) than in later stages of the moult cycle (about 9%). This reflected probably an increase in the degree of calcification, i.e. in the calcium carbonate content of the exoskeleton. As a fraction of total C, inorganic C reached maximum values of 17 and 20% in the crab I and II instars, respectively. The energy content of juvenile spider crabs was estimated independently from organic C and biochemical constituents, with a significant correlation between these values. However, the former estimates of energy were, on the average, significantly lower than the latter (slope of the regression ≠1). Since organic C should be a reliable integrator of organic substances, but the sum of protein, lipid, chitin, and carbohydrates amounted to only 60 91% of AFW, it is concluded that the observed discrepancy between these two estimates of energy was caused by energy from biochemical constituents that had not been determined in our analyses. Thus, energy values obtained from these biochemical fractions alone may underestimate the actual amount of organic matter and energy. Respiration per individual in juvenile spider crabs was higher than that in larval stages of the same species (previous studies), but their W-specific values of oxygen consumption (QO2) were lower than in conspecific larvae (0.6 2μg O2·[mg W]-1). QO2 showed a consistent periodic pattern in relation to the moult cycle: maximum values in early postmoult, followed by a rapid decrease, and constant values in the intermoult and premoult phases. This variation is interpreted as an effect mainly of cyclic changes in the amounts of cuticle materials which are metabolically inactive. From growth and respiration values (both expressed in units of organic C), net growth efficiency, K2, values may be calculated. In contrast to previous findings in larval stages, K2 showed an increasing trend during growth of the first two juvenile instars of H. araneus.

  1. Paternity analyses in wild-caught and laboratory-reared Caribbean cricket females reveal the influence of mating environment on post-copulatory sexual selection.

    PubMed

    Oneal, E; Knowles, L L

    2015-12-01

    Polyandry is ubiquitous in insects and provides the conditions necessary for male- and female-driven forms of post-copulatory sexual selection to arise. Populations of Amphiacusta sanctaecrucis exhibit significant divergence in portions of the male genitalia that are inserted directly into the female reproductive tract, suggesting that males may exercise some post-copulatory control over fertilization success. We examine the potential for male-male and male-female post-copulatory interactions to influence paternity in wild-caught females of A. sanctaecrucis and contrast our findings with those obtained from females reared in a high-density laboratory environment. We find that female A. sanctaecrucis exercise control by mating multiple times (females mount males), but that male-male post-copulatory interactions may influence paternity success. Moreover, post-copulatory interactions that affect reproductive success of males are not independent of mating environment: clutches of wild-caught females exhibit higher sire diversity and lower paternity skew than clutches of laboratory-reared females. There was no strong evidence for last male precedence in either case. Most attempts at disentangling the contributions of male-male and male-female interactions towards post-copulatory sexual selection have been undertaken in a laboratory setting and may not capture the full context in which they take place--such as the relationship between premating and post-mating interactions. Our results reinforce the importance of designing studies that can capture the multifaceted nature of sexual selection for elucidating the role of post-copulatory sexual selection in driving the evolution of male and female reproductive traits, especially when different components (e.g. precopulatory and post-copulatory interactions) do not exert independent effects on reproductive outcomes. PMID:26348983

  2. Use of PIT tags to assess individual heterogeneity of laboratory-reared juveniles of the endangered Cumberlandian combshell (Epioblasma brevidens) in a mark-recapture study.

    PubMed

    Hua, Dan; Jiao, Yan; Neves, Richard; Jones, Jess

    2015-03-01

    The federally endangered Cumberlandian combshell (Epioblasma brevidens) was propagated and reared to taggable size (5-10 mm), and released to the Powell River, Tennessee, to augment a relict population. Methodology using passive integrated transponder (PIT) tags on these mussels greatly facilitated the detection process. The overall mean detection probability and survival rate of released individuals reached 97.8 to 98.4% and 99.7 to 99.9% (per month), respectively, during nine successive recapture occasions in the 2-year study period, regardless of seasonality. Nonhierarchical models and hierarchical models incorporating individual and seasonal variations through a Bayesian approach were compared and resulted in similar performance of prediction for detection probability and survival rate of mussels. This is the first study to apply the mark-recapture method to laboratory-reared mussels using PIT tags and stochastic models. Quantitative analyses for individual heterogeneity allowed examination of demographic variance and effects of heterogeneity on population dynamics, although the individual and seasonal variations were small in this study. Our results provide useful information in implementing conservation strategies of this faunal group and a framework for other species or similar studies. PMID:25798225

  3. Use of PIT tags to assess individual heterogeneity of laboratory-reared juveniles of the endangered Cumberlandian combshell (Epioblasma brevidens) in a mark–recapture study

    PubMed Central

    Hua, Dan; Jiao, Yan; Neves, Richard; Jones, Jess

    2015-01-01

    The federally endangered Cumberlandian combshell (Epioblasma brevidens) was propagated and reared to taggable size (5–10 mm), and released to the Powell River, Tennessee, to augment a relict population. Methodology using passive integrated transponder (PIT) tags on these mussels greatly facilitated the detection process. The overall mean detection probability and survival rate of released individuals reached 97.8 to 98.4% and 99.7 to 99.9% (per month), respectively, during nine successive recapture occasions in the 2-year study period, regardless of seasonality. Nonhierarchical models and hierarchical models incorporating individual and seasonal variations through a Bayesian approach were compared and resulted in similar performance of prediction for detection probability and survival rate of mussels. This is the first study to apply the mark–recapture method to laboratory-reared mussels using PIT tags and stochastic models. Quantitative analyses for individual heterogeneity allowed examination of demographic variance and effects of heterogeneity on population dynamics, although the individual and seasonal variations were small in this study. Our results provide useful information in implementing conservation strategies of this faunal group and a framework for other species or similar studies. PMID:25798225

  4. Pathogenecity of Ichthyophonus hoferi for laboratory-reared Pacific herring (Clupea pallasi) and its early appearance in wild Puget Sound herring

    USGS Publications Warehouse

    Kocan, R.; Hershberger, P.; Mehl, T.; Elder, N.; Bradley, M.; Wildermuth, D.; Stick, K.

    1999-01-01

    Laboratory-reared pathogen-free Pacific herring were exposed to pure cultures of Ichthyophonus hoferi, and reproduced the disease seen in naturally infected fish--thus fulfilling Koch's Postulates. Pathogen-free herring used in this study were reared from artificially spawned eggs incubated in filtered, UV-sterilized seawater, eliminating the variables associated with multiple infections, which are common in wild herring. Wild free-ranging herring were captured monthly from June through October by dip net from 'herring balls' located in the northern Puget Sound. I. hoferi infections were identified in these fish soon after metamorphoses, about 4 mo post-hatch. The prevalence increased from 5 to 6% in 0-yr fish to 24% in 1-yr-old fish to 50 to 70% in fish over 2 yr old, with no associated increase in mortality. The route of natural transmission to wild herring was not determined, but carnivorous fish became infected and died when they were experimentally fed tissues infected with the organism. In vitro culture of tissues was the most sensitive method for identifying both clinical and subclinical infections.

  5. Pathogenicity of Ichthyophonus hoferi for laboratory-reared Pacific herring Clupea pallasi and its early appearance in wild Puget Sound herring

    USGS Publications Warehouse

    Kocan, R.M.; Hershberger, P.; Mehl, T.; Elder, N.; Bradley, M.; Wildermuth, D.; Stick, K.

    1999-01-01

    Laboratory-reared pathogen-free Pacific herring were exposed to pure cultures of Ichthyophonus hoferi, and reproduced the disease seen in naturally infected fish - thus fulfilling Koch's Postulates. Pathogen-free herring used in this study were reared from artificially spawned eggs incubated in filtered, UV-sterilized seawater, eliminating the variables associated with multiple infections, which are common in wild herring. Wild free-ranging herring were captured monthly from June through October by dip net from 'herring balls' located in the northern Puget Sound. I. hoferi infections were identified in these fish soon after metamorphoses, about 4 mo post-hatch. The prevalence increased from 5 to 6% in 0-yr fish to 24% in 1-yr-old fish to 50 to 70% in fish over 2 yr old, with no associated increase in mortality. The route of natural transmission to wild herring was not determined, but carnivorous fish became infected and died when they were experimentally fed tissues infected with the organism. In vitro culture of tissues was the most sensitive method for identifying both clinical and subclinical infections.

  6. LABORATORY EVALUATION OF NOX REDUCTION TECHNIQUES FOR REFINERY CO BOILERS

    EPA Science Inventory

    The paper gives results of work to develop and demonstrate, in the laboratory, NOx reduction techniques that would be suited to petroleum refinery CO boilers. (Note: Most refineries have one or more CO boilers to incinerate off-gases from catalytic cracking. These devices constit...

  7. In Situ Techniques for Monitoring Electrochromism: An Advanced Laboratory Experiment

    ERIC Educational Resources Information Center

    Saricayir, Hakan; Uce, Musa; Koca, Atif

    2010-01-01

    This experiment employs current technology to enhance and extend existing lab content. The basic principles of spectroscopic and electroanalytical techniques and their use in determining material properties are covered in some detail in many undergraduate chemistry programs. However, there are limited examples of laboratory experiments with in…

  8. Laboratory technique for coloring titanium abutments to improve esthetics.

    PubMed

    Wadhwani, Chandur P K; O'Brien, Richard; Kattadiyil, Mathew T; Chung, Kwok-Hung

    2016-04-01

    Titanium alloys are used for implant abutments onto which prostheses are attached. One major disadvantage of titanium abutments is their esthetics; the metallic gray color may show through the restorative material or through surrounding tissues. A laboratory technique using readily available household items is described that can alter the abutment color by anodization. PMID:26723096

  9. Development of liquid larval diet with modified rearing system for Bactrocera dorsalis (Hendel) (Diptera:Tephritidae) for the application of sterile insect technique

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A liquid larval diet and its rearing system have been developed for mass rearing of Bactrocera dorsalis (Hendel) in Hawaii. Rearing facility in Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Dhaka, Bangladesh, modified protein source from brewer's yeast to a combinat...

  10. Pathfinder Rear Ramp

    NASA Technical Reports Server (NTRS)

    1997-01-01

    Mars Pathfinder's rear rover ramp can be seen successfully unfurled in this image, taken at the end of Sol 2 by the Imager for Mars Pathfinder (IMP). This ramp was later used for the deployment of the microrover Sojourner, which occurred at the end of Sol 2. Areas of a lander petal and deflated airbag are visible at left. The image helped Pathfinder scientists determine that the rear ramp was the one to use for rover deployment. At upper right is the rock dubbed 'Barnacle Bill,' which Sojourner will later study.

    Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C.

  11. Laboratory survival, growth, and digestive gland histologic features of squids reared on living and non-living fish diets.

    PubMed

    DiMarco, F P; Turk, P E; Scimeca, J M; Browning, W J; Lee, P G

    1993-06-01

    The effects of non-living diets on the survival, growth, and digestive gland histologic features of the bigfin reef squid (Sepioteuthis lessoniana Lesson, 1830) cultured in the laboratory were evaluated during one-half of their life cycle (95 days). Two groups of squids (n = 16 per group) were held in closed seawater systems with similar water volume, temperature, salinity, water filtration, and water flow velocities. Food for the control group consisted of live, freely swimming fish (Cyprinodon variagatus); the test group was trained to grab freshly dead fish (days 1 to 45) and then thawed, frozen fish (days 46 to 95). The two groups were evaluated for differences in (1) food intake, (2) survival, (3) growth (wet weight, mantle length, instantaneous growth rate), (4) morphologic (mantle thickness in four locations, digestive gland weight), and (5) digestive gland histologic features (indices for nuclear density and relative vacuolar density). Unexpectedly, no significant differences were found between the two groups. Mean wet weight increased from 32.1 g to 342.9 g for the control group and from 58.6 g to 372.0 g for the group fed dead food. The results demonstrate that laboratory-cultured squids can survive and grow when fed dead fish (fresh or frozen) as well as live fish without adverse effects on growth, survival, or digestive gland histologic features. PMID:8355482

  12. Additional moults into ‘elongatus’ males in laboratory-reared Polydesmus angustus Latzel, 1884 (Diplopoda, Polydesmida, Polydesmidae) – implications for taxonomy

    PubMed Central

    David, Jean-Francois; Geoffroy, Jean-Jacques

    2011-01-01

    Abstract The number of stadia during post-embryonic development is supposed to be fixed in most species of the millipede order Polydesmida. For the first time since 1928, additional moults were observed in two males of Polydesmus angustus Latzel, 1884 reared in the laboratory. These ‘elongatus’ males sensu Verhoeff reached stadium IX instead of stadium VIII, with addition of a further podous ring (32 pairs of legs). One male had well-developed gonopods at stadium VIII, which regressed at stadium IX; the other had no gonopods at stadium VIII, which developed at stadium IX. The two cases correspond to the ‘regressionis’ and ‘progressionis’ forms described by Verhoeff in Polydesmus complanatus (Linnaeus, 1761), which confirms entirely his results. Additional moults appear to be associated with small body sizes and possible underlying mechanisms are discussed. Comparisons between millipede orders indicate that post-embryonic development is less strictly canalized in Polydesmida than in Chordeumatida. This implies that the adult number of body rings is of limited taxonomic value in Polydesmida and should not be viewed as a character of generic importance. PMID:22303094

  13. Effects of zinc on the structure and growth dynamics of a natural freshwater phytoplankton assemblage reared in the laboratory.

    PubMed

    Loez, C R; Topalián, M L; Salibián, A

    1995-01-01

    The response of a natural phytoplankton assemblage to different concentrations of Zn(2+) was evaluated by means of a static laboratory bioassay. Aliquots of surface water, taken in autumn from a non-polluted point of the Reconquista River (Buenos Aires, Argentia), were incubated in mineral nutrient media containing 2.5, 10 or 25 mg litre(-1) of Zn(2+) (as zinc chloride). The comparative structure and dynamics of the communities were followed through periodic physico-chemical and biological analyses of samples taken during 24 days of incubation. Under the experimental conditions of the bioassays, the existence of several Zn(2+) tolerant algal species was shown: the most important of them were Chlorella vulgaris Beij. (Chlorophyceae, Chlorococcales), Nitzschia palea (Kütz.) Smith and Gomphonema parvulum (Kütz.) Kütz. (Bacillariophyceae). It was also demonstrated that the algal responses to Zn(2+) were selectively concentration dependent: at 2.5 and 10 mg litre(-1) a stimulatory effect was observed in the diatoms; at 25 mg litre(-1), diatom toxicity occurred. In contrast, Chlorophyceae growth was stimulated at the maximal Zn level. In general, the diversity, richness and equitability of the community were adversely affected by Zn in a concentration-dependent fashion. PMID:15091539

  14. Serum Clinical Biochemical and Hematologic Reference Ranges of Laboratory-Reared and Wild-Caught Xenopus laevis

    PubMed Central

    Wilson, Sabrina; Felt, Stephen; Torreilles, Stéphanie; Howard, Antwain; Behan, Colleen; Moorhead, Roberta; Green, Sherril

    2011-01-01

    The South African clawed frogs Xenopus laevis and X. tropicalis are fully aquatic amphibians and well-established animal models. Because genetically engineered laboratory Xenopus are now being produced, the establishment of normal reference ranges for serum biochemical and hematologic parameters is essential for phenotyping and as a diagnostic aide. We determined normal reference ranges for hematologic values from 3 populations of X. laevis: wild-caught frogs (n = 43) and frogs from 2 commercial sources (A, n = 166; B, n = 109). For serum biochemistry, we determined normal reference ranges for frogs from source A and wild-caught frogs divided by sex and season. Significant differences across populations were found in WBC and RBC counts, hemoglobin concentration, hematocrit, mean corpuscular hemoglobin concentration, and mean corpuscular volume. Among serum biochemical analytes, significant differences were found for albumin:globulin ratio, anion gap, and concentrations of albumin, globulin, total protein, lipase, alanine transaminase, γ-glutamyl transpeptidase; creatine phosphokinase; indirect, direct, and total bilirubin; cholesterol, low-density lipoprotein lipase, carbon dioxide, glucose, lactacte dehydrogenase, calcium, chloride, and sodium. We hypothesize that these differences can be attributed to differences in water quality, habitat, ambient temperature, diet, sex, recent transport or shipment, and genetic background. However, testing that hypothesis is beyond the scope of the current study. In addition, clinical chemistry and hematologic reference range values Xenopus laevis are quite distinct from those for other species and are most consistent with the only values published for another fully aquatic amphibian, the Eastern hellbender (Cryptobranchus alleganiensis). PMID:22330708

  15. Polymerase Chain Reaction Detection of Pseudoloma neurophilia, a Common Microsporidian of Zebrafish (Danio rerio) Reared in Research Laboratories

    PubMed Central

    Whipps, Christopher M.; Kent, Michael L.

    2006-01-01

    One of the most prevalent pathogens found in zebrafish (Danio rerio) research facilities is the microsporidian parasite Pseudoloma neurophilia. Infections occur primarily in the spinal cord and are associated with emaciation and scoliotic changes. It is unclear why P. neurophilia is so widespread among research colonies, although transfer of infected animals and eggs between laboratories is a likely contributor. In addition to preventing the spread of this pathogen among facilities, it is desirable to have parasite-free fish for use in experiments. Therefore we have developed a polymerase chain reaction (PCR)-based diagnostic test for P. neurophilia. Compared with conventional diagnostic methods, PCR diagnosis is rapid, allows for screening of large numbers of fish, and can be applied to eggs, water filtrates, biofilms, and other samples. Using PCR primers specific to the small subunit ribosomal DNA of P. neurophilia, the test was consistently capable of detecting 10 spores per reaction and often as few as 0.1 spore per reaction and did not cross-react with other selected microsporidian species from fish. We recommend this PCR diagnostic assay for use by the research community to determine the presence (or absence) of P. neurophilia in colonies and for screening fish shipped between facilities, especially when parasite-free fish are required for experiments. Furthermore, we currently are using this PCR method to investigate the potential role of vertical transmission in the spread of P. neurophilia. PMID:16539333

  16. A Laboratory Method for Rearing Tea Green Leafhopper, Empoasca onukii Matsuda by Using Tea Seedlings as Oviposition Sites and as Food

    NASA Astrophysics Data System (ADS)

    Kosugi, Yukio

    In this study,I have developed a method for rearing tea green leafhopper, Empoasca onukii Matsuda by using tea seedlings. Tea seeds were prepared by incubating tea seeds at 5 ℃ for 30 daysand planted these seeds in agar gel.The germination rate of the seedlings on the 28th postincubation (at 25 ℃) days was 89.5%. When the length of new shoots was 7-8 cm, the seedlings were replanted in a cup containing agar gel. The cups were covered with a plastic cap and used as a rearing case. One adult female was captured from the tea fields and reared on the rearing case. On an average, 34.7 nymphs were found to breed on this rearing case. However, when the number of adult females in the rearing case was increased, the number of nymphs tended to decrease. Rearing of 1-2 adult males and females resulted in the breeding of more than 20 nymphs, ranging from the first to the fifth generation.

  17. Emission of nitric oxide from soil: laboratory vs. micrometeorological techniques

    NASA Astrophysics Data System (ADS)

    Meixner, F. X.; Mayer, J.-C.; Bargsten, A.; Andreae, M. O.

    2009-04-01

    High quality field measurements of the surface exchange of nitric oxide (NO) between soils and the atmosphere usually demand complex instrumentation and considerable power supplies. The latter often impedes such flux measurements at remote sites. However, soil samples could be taken everywhere, and consecutive parameterization of NO emission rates (derived from laboratory incubation of these soil samples) and up-scaling through independent data of soil properties (e.g. soil moisture, soil temperature, soil texture) is an effective alternative to quantify NO emission from soils, particularly from (very) remote sites. These laboratory derived NO emission rates have quite frequently verified through field measurements by the dynamic chamber technique. Here, we will present the first intercomparison between the laboratory and a micrometeorological (gradient) technique. In August 2006, the field experiment LIBRETTO (LIndenBerg REacTive Trace gas prOfiles) was carried out in cooperation with the German Meteorological Service (DWD) at the field site of the Richard Aßmann Observatory in Lindenberg. At 0.15 m and 2.0 m, concentrations of the trace gases O3, NO and NO2 were measured. Applying the modified Bowen ration technique to the measured concentration differences and the directly measured sensible heat flux (eddy covariance data from DWD) yielded the field surface fluxes of the trace gases (corrected for their flux divergence due to fast photochemical reactions during the vertical turbulent transport). Soil humidity and temperature in the top soil were measured at a separate plot at the field site. Mixed soil samples were taken in May 2008 at the LIBRETTO field site, air-dried, sieved (

  18. Evaluating the potential of the sterile insect technique for malaria control: relative fitness and mating compatibility between laboratory colonized and a wild population of Anopheles arabiensis from the Kruger National Park, South Africa

    PubMed Central

    2011-01-01

    Background The successful suppression of a target insect population using the sterile insect technique (SIT) partly depends on the premise that the laboratory insects used for mass rearing are genetically compatible with the target population, that the mating competitiveness of laboratory reared males is at least comparable to that of their wild counterparts, and that mass rearing and sterilization processes do not in themselves compromise male fitness to a degree that precludes them from successfully competing for mates in the wild. This study investigated the fitness and sexual cross-compatibility between samples of field collected and laboratory reared An. arabiensis under laboratory conditions. Results The physiological and reproductive fitness of the MALPAN laboratory strain is not substantially modified with respect to the field population at Malahlapanga. Further, a high degree of mating compatibility between MALPAN and the Malahlapanga population was established based on cross-mating experiments. Lastly, the morphological characteristics of hybrid ovarian polytene chromosomes further support the contention that the MALPAN laboratory colony and the An. arabiensis population at Malahlapanga are genetically homogenous and therefore compatible. Conclusions It is concluded that the presence of a perennial and isolated population of An. arabiensis at Malahlapanga presents a unique opportunity for assessing the feasibility of SIT as a malaria vector control option. The MALPAN laboratory colony has retained sufficient enough measures of reproductive and physiological fitness to present as a suitable candidate for male sterilization, mass rearing and subsequent mass release of sterile males at Malahlapanga in order to further assess the feasibility of SIT in a field setting. PMID:22041133

  19. Coupled explosive/structure computational techniques at Sandia National Laboratories

    SciTech Connect

    Preece, D.S.; Attaway, S.W.; Swegle, J.W.

    1997-06-01

    Simulation of the effects of explosives on structures is a challenge because the explosive response can best be simulated using Eulerian computational techniques and structural behavior is best modeled using Lagrangian methods. Due to the different methodology of the two computational techniques and code architecture requirements, they are usually implemented in different computer programs. Explosive and structure modeling in two different codes make it difficult or next to impossible to do coupled explosive/structure interaction simulations. Sandia National Laboratories has developed two techniques for solving this problem. The first is called Smoothed Particle Hydrodynamics (SPH), a relatively new gridless method comparable to Eulerian, that is especially suited for treating liquids and gases such as those produced by an explosive. The SPH capability has been fully implemented into the transient dynamics finite element (Lagrangian) codes PRONTO-2D and -3D. A PRONTO-3D/SPH simulation of the effect of a blast on a protective-wall barrier is presented in this paper. The second technique employed at Sandia uses a new code called Zapotec that combines the 3-D Eulerian code CTH and the Lagrangian code PRONTO-3D with minimal changes to either code. CTH and PRONTO-3D are currently executing on the Sandia Terraflops machine (9000 Pentium Pro processors). Eulerian simulations with 100 million cells have been completed on the current configuration of the machine (4500 Pentium Pro processors). The CTH and PRONTO-3D combination will soon be executing in a coupled fashion on this machine.

  20. Laboratory rearing of bed bugs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The resurgence of bed bugs Cimex lectularius L. in the United States and worldwide has resulted in an increase in research by university, government, and industry scientists directed at the biology and control of this blood-sucking pest. A need has subsequently arisen for producing sufficient biolog...

  1. Laboratory Detection of Respiratory Viruses by Automated Techniques

    PubMed Central

    Pérez-Ruiz, Mercedes; Pedrosa-Corral, Irene; Sanbonmatsu-Gámez, Sara; Navarro-Marí, José-María

    2012-01-01

    Advances in clinical virology for detecting respiratory viruses have been focused on nucleic acids amplification techniques, which have converted in the reference method for the diagnosis of acute respiratory infections of viral aetiology. Improvements of current commercial molecular assays to reduce hands-on-time rely on two strategies, a stepwise automation (semi-automation) and the complete automation of the whole procedure. Contributions to the former strategy have been the use of automated nucleic acids extractors, multiplex PCR, real-time PCR and/or DNA arrays for detection of amplicons. Commercial fully-automated molecular systems are now available for the detection of respiratory viruses. Some of them could convert in point-of-care methods substituting antigen tests for detection of respiratory syncytial virus and influenza A and B viruses. This article describes laboratory methods for detection of respiratory viruses. A cost-effective and rational diagnostic algorithm is proposed, considering technical aspects of the available assays, infrastructure possibilities of each laboratory and clinic-epidemiologic factors of the infection PMID:23248735

  2. Aseptic laboratory techniques: volume transfers with serological pipettes and micropipettors.

    PubMed

    Sanders, Erin R

    2012-01-01

    Microorganisms are everywhere - in the air, soil, and human body as well as on inanimate surfaces like laboratory benches and computer keyboards. The ubiquity of microbes creates a copious supply of potential contaminants in a laboratory. To ensure experimental success, the number of contaminants on equipment and work surfaces must be minimized. Common among many experiments in microbiology are techniques involving the measurement and transfer of cultures containing bacterial cells or viral particles. To do so without contacting non-sterile surfaces or contaminating sterile media requires (1) preparing a sterile workspace, (2) precisely setting and accurately reading instruments for aseptic transfer of liquids, and (3) properly manipulating instruments, cultures flasks, bottles and tubes within a sterile field. Learning these procedures calls for training and practice. At first, actions should be slow, deliberate, and controlled with the goal being for aseptic technique to become second nature when working at the bench. Here we present the steps for measuring volumes using serological pipettes and micropipettors within a sterile field created by a Bunsen burner. Volumes range from microliters (μl) to milliliters (ml) depending on the instrument used. Liquids commonly transferred include sterile broth or chemical solutions as well as bacterial cultures and phage stocks. By following these procedures, students should be able to: ·Work within the sterile field created by the Bunsen burner flame. ·Use serological pipettes without compromising instrument sterility. ·Aspirate liquids with serological pipettes, precisely reading calibrated volumes by aligning the meniscus formed by the liquid to the graduation marks on the pipette. ·Keep culture bottles, flasks, tubes and their respective caps sterile during liquid transfers. ·Identify different applications for plastic versus glass serological pipettes. ·State accuracy limitations for micropipettors. ·Precisely and accurately set volumes on micropipettors. ·Know how to properly use the first and second stop on a micropipettor to aspirate and transfer correct volumes. PMID:22688118

  3. Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors

    PubMed Central

    Sanders, Erin R.

    2012-01-01

    Microorganisms are everywhere - in the air, soil, and human body as well as on inanimate surfaces like laboratory benches and computer keyboards. The ubiquity of microbes creates a copious supply of potential contaminants in a laboratory. To ensure experimental success, the number of contaminants on equipment and work surfaces must be minimized. Common among many experiments in microbiology are techniques involving the measurement and transfer of cultures containing bacterial cells or viral particles. To do so without contacting non-sterile surfaces or contaminating sterile media requires (1) preparing a sterile workspace, (2) precisely setting and accurately reading instruments for aseptic transfer of liquids, and (3) properly manipulating instruments, cultures flasks, bottles and tubes within a sterile field. Learning these procedures calls for training and practice. At first, actions should be slow, deliberate, and controlled with the goal being for aseptic technique to become second nature when working at the bench. Here we present the steps for measuring volumes using serological pipettes and micropipettors within a sterile field created by a Bunsen burner. Volumes range from microliters (μl) to milliliters (ml) depending on the instrument used. Liquids commonly transferred include sterile broth or chemical solutions as well as bacterial cultures and phage stocks. By following these procedures, students should be able to: •Work within the sterile field created by the Bunsen burner flame. •Use serological pipettes without compromising instrument sterility.• Aspirate liquids with serological pipettes, precisely reading calibrated volumes by aligning the meniscus formed by the liquid to the graduation marks on the pipette. •Keep culture bottles, flasks, tubes and their respective caps sterile during liquid transfers. •Identify different applications for plastic versus glass serological pipettes. •State accuracy limitations for micropipettors. •Precisely and accurately set volumes on micropipettors. •Know how to properly use the first and second stop on a micropipettor to aspirate and transfer correct volumes. PMID:22688118

  4. Effect of Parasitoid: Host Ratio and Parasitoid and Host Group Size on Fitness of Spathius galinae (Hymenoptera: Braconidae), a Parasitoid of Emerald Ash Borer (Coleoptera: Buprestidae): Implications for Mass-Rearing

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Producing insect natural enemies in laboratories or insectaries for biological pest control is often expensive, and developing cost-effective rearing techniques is a goal of many biological control programs. Spathius galinae Belokobylskij and Strazenac (Hymenoptera: Braconidae), a recently described...

  5. A Novel Experimental Technique to Simulate Pillar Burst in Laboratory

    NASA Astrophysics Data System (ADS)

    He, M. C.; Zhao, F.; Cai, M.; Du, S.

    2015-09-01

    Pillar burst is one type of rockburst that occurs in underground mines. Simulating the stress change and obtaining insight into the pillar burst phenomenon under laboratory conditions are essential for studying the rock behavior during pillar burst in situ. To study the failure mechanism, a novel experimental technique was proposed and a series of tests were conducted on some granite specimens using a true-triaxial strainburst test system. Acoustic emission (AE) sensors were used to monitor the rock fracturing process. The damage evolution process was investigated using techniques such as macro and micro fracture characteristics observation, AE energy evolution, and b value analysis and fractal dimension analysis of cracks on fragments. The obtained results indicate that stepped loading and unloading simulated the pillar burst phenomenon well. Four deformation stages are divided as initial stress state, unloading step I, unloading step II, and final burst. It is observed that AE energy has a sharp increase at the initial stress state, accumulates slowly at unloading steps I and II, and increases dramatically at peak stress. Meanwhile, the mean b values fluctuate around 3.50 for the first three deformation stages and then decrease to 2.86 at the final stage, indicating the generation of a large amount of macro fractures. Before the test, the fractal dimension values are discrete and mainly vary between 1.10 and 1.25, whereas after failure the values concentrate around 1.25-1.35.

  6. View of south rear and east sides, facing northnorthwest ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of south rear and east sides, facing north-northwest - International Longshoremen's & Warehousemen's Union Hall, Naval Civil Engineering Laboratory, Port Hueneme Road, Port Hueneme, Ventura County, CA

  7. Biology, life history and laboratory rearing of Spathius galinae (Hymenoptera: Braconidae), a larval parasitoid of the invasive Emerald Ash Borer (Coleoptera: Buprestidae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Spathius galinae Belokobylskij & Strazajac is a recently described parasitoid of the emerald ash borer, Agrilus plannipennis Fairmaire, in the Russian Far East, and is currently being considered for biocontrol introduction in the US. Using A. planipennis larvae reared with freshly cut ash (Fraxinus ...

  8. Impacts of extended laboratory rearing on female fitness in Florida colonies of the parasitoid spalangia cameroni (Hymenoptera: Pteromalidae) with an analysis of wolbachia strains

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Spalangia cameroni is used as a biological control agent of filth flies. These parasitoids are reared commercially, but little is known about the impact of colony age on host-seeking and life history parameters. Host-seeking in equine shavings and manure was analyzed with two colony ages established...

  9. Manual of Basic Techniques for a Health Laboratory.

    ERIC Educational Resources Information Center

    World Health Organization, Geneva (Switzerland).

    Described are basic laboratory methods for diagnosing and investigating diseases of importance to developing countries. Intended primarily for the training of technicians who will work in peripheral laboratories, the manual is designed so that student laboratory assistants can be taught to use it with minimal supervision from a teacher. The…

  10. EM techniques for archaeological laboratory experiments: preliminary results

    NASA Astrophysics Data System (ADS)

    Capozzoli, Luigi; De Martino, Gregory; Giampaolo, Valeria; Raffaele, Luongo; Perciante, Felice; Rizzo, Enzo

    2015-04-01

    The electromagnetic techniques (EM) are based on the investigation of subsoil geophysical parameters and in the archaeological framework they involve in studying contrasts between the buried cultural structures and the surrounding materials. Unfortunately, the geophysical contrast between archaeological features and surrounding soils sometimes are difficult to define due to problems of sensitivity and resolution both related on the characteristic of the subsoil and the geophysical methods. For this reason an experimental activity has been performed in the Hydrogeosite laboratory addressed on the assessment of the capability of geophysical techniques to detect archeological remains placed in the humid/saturated subsoil. At Hydrogeosite Laboratory of CNR-IMAA, a large scale sand-box is located, consisting on a pool shape structures of 230m3 where archaeological remains have been installed . The remains are relative to a living environment and burial of Roman times (walls, tombs, roads, harbour, etc.) covered by sediments. In order to simulate lacustrine and wetland condition and to simulate extreme events (for example underwater landslide, fast natural erosion coast, etc.) the phreatic level was varied and various acquisitions for the different scenarios were performed. In order to analyze the EM behavior of the buried small archaeological framework, ground penetrating radar (GPR) and electrical resistivity tomographies were performed. With GPR, analysis in time domain and frequency domain were performed and coupled to information obtained through resistivity analysis with the support of numerical simulations used to compare the real data with those modeled. A dense grid was adopted for 400 and 900 MHz e-m acquisitions in both the directions, the maximum depth of investigation was limited and less than 3 meters. The same approach was used for ERT acquisition where different array are employed, in particular 3D configuration was used to carry out a 3D resistivity model. The integration of electric and electromagnetic data allowed us to overcome the limits of each technique, especially in terms of resolution and depth, in humid/saturated conditions was investigated and the effectiveness of three-dimensional acquisitions was studied to better explore archeological sites and reduce the uncertainties related on the interpretation of geophysical analysis. The complexity of the relationship between archaeological features in the subsoil and their geophysical response requires efforts in the interpretation of resulting data. Reference Campana S. and Piro, S., (2009): Seeing the unseen - Geophysics and landscape archaeology., CRC Press, London, 2. No. of pages: 376. ISBN: 978-0-415-44721-8. Conyers, L. and Goodman, D., (1997): Ground-Penetrating Radar: An Introduction for Archaeologists. Walnut Creek, Calif.: AltaMira Press. Davis, J.L. and Annan, A.P. (1989): Ground-penetrating radar for high-resolution mapping of soil and rock stratigraphy. Geophysical Prospecting, 37, 531-551.

  11. A laboratory technique for thermal emission measurement of hydrated minerals.

    PubMed

    Baldridge, A M; Christensen, P R

    2009-06-01

    Laboratory emission spectra are measured at Arizona State University's Mars Space Flight Facility for comparison to remotely sensed data from Earth and Mars. Such emission spectroscopy using an interferometric spectrometer measures the energy of the sample, including reflected and emitted background sources. The detector is uncooled at ambient temperature, which produces a very low signal when measuring the energy from a sample that has a temperature close to its own. In order to increase the energy difference between the sample and the detector, thereby increasing the signal received by the detector, samples are typically heated to between 60 and 80 degrees C for several hours prior to measurement. While this method is acceptable for most rock and mineral samples, some hydrous minerals dehydrate quickly at low relative humidity and temperatures above room temperature. This change is evident in both the physical appearance of the mineral and in the position and shape of its spectral absorptions. One solution to this problem is to heat samples to lower temperatures (e.g., 40 degrees C) for only a short time period. However, this approach results in a low signal from the sample and does not always avoid dehydration. For this reason, we have developed a technique for measuring and calibrating emission spectra of hydrated minerals that involves cooling samples to well below the temperature of the detector, which avoids dehydration, while creating a large delta temperature and a strong signal from the sample. Our method allows for accurate library spectra, with discrete, pronounced spectral features (high spectral contrast), of hydrated minerals that can be used for comparison to planetary surfaces. PMID:19531295

  12. Calling behavior of mass-reared and wild Anastrepha serpentina (Diptera: Tephritidae).

    PubMed

    Castrejón-Gómez, Victor R; Lascares, Shaila; Malo, Edi A; Toledo, Jorge; Rojas, Julio C

    2007-08-01

    The calling behavior of mass-reared and wild males of Anastrepha serpentina (Wiedemann) (Diptera: Tephritidae) was studied both in the laboratory and in field cage tests. In the laboratory, density (1, 5, and 10 males per container), age, and hour of day significantly affected calling behavior. Mass-reared males called independently of density, whereas wild males only called at densities of 5 and 10 individuals. Males of both strains started calling when they were 5-7 d old. The daily pattern of male calling was similar in both strains, starting at 0730 hours, and reaching a peak at 1330-1630 hours. Field cage tests showed that mass-reared males started calling when they were 5d old; the period of peak calling was when males were 8-9 d old. In contrast, wild males began calling when they were 10 d old, reaching peaks when males were 13, 15, and 18 d old. Wild males tended to form leks to call during each day of the experiment, whereas mass-reared males only formed leks during 2 d, both strains displaying very low levels. During field cage tests, males, independently of strain, displayed two calling peaks, one peak in the morning and one peak in the afternoon, whereas males observed in the laboratory only showed a single calling peak. The results are discussed in view of the effects of mass rearing A. serpentina males in relation to potential use of the sterile insect technique. PMID:17849867

  13. Diets, equipment, and techniques for maintaining crawfish in the laboratory

    USGS Publications Warehouse

    Tarshis, I.B.

    1978-01-01

    One commercial and 4 laboratory prepared extruded, water-stable diets were fed 3 times a week in 1-g portions to juvenile male and female White River crawfish, Procambarus acutus acutus (Girard), for 10 weeks. The. binding material in the laboratory preparation was alginate (Kelgin), whereas that in the commercial preparation was starch. No statistically significant weight differences developed between the groups of crawfish at the end of the 10-week period; all 5 diets were found satisfactory for feeding and maintaining P. acutus acutus in the laboratory, and all test crawfish survived throughout the experimental period. Weight gains were highest in a diet containing 50.5% protein; intermediate in those fed a diet with 46.0% protein; and lowest in those fed diets with 31.7 or 36.3% protein. Crawfish fed the commercial preparation of one of the 46.0% protein diets showed a slightly but not significantly higher weight gain than those fed the laboratory preparation of the diet. In an evaluation of the water stability of 5 commercially prepared animal chow diets and the commercial extruded diet, 2 of the commercial diets disintegrated after one hour exposure in water and the other 3 became bloated after one hour and remained on the surface throughout the 24-hour test. The commercial extruded diet maintained its water stability for the full 24 hours. The commercial preparation of the 46.0% protein diet was successfully used under laboratory conditions for feeding and maintaining the following crawfishes: Cambarellus shufeldtii (Faxon), Cambarus acuminatus Faxon, Orconectes limosus (Rafinesque), O. virilis (Hagen), Procambarus clarkii (Girard), and P. spiculifer (Le conte). In longevity experiments Cambarus diogenes diogenes Girard and Procambarus hinei (Ortmann) now have survived for 8 months on this diet in the laboratory.

  14. Mental Practice as a Technique to Improve Laboratory Skill Development.

    ERIC Educational Resources Information Center

    Beasley, Warren F.; Heikkinen, Henry W.

    1983-01-01

    Reports research into the design and application of physical and mental practice strategies for the development of laboratory psychomotor skills of freshman chemistry students (N=360). Design of instruction materials used (focusing skills using balance, pipet, and buret and skills in quantitative volumetric analysis), evaluation of strategies, and…

  15. A new larval tray and rack system for improved mosquito mass rearing.

    PubMed

    Balestrino, F; Benedict, M Q; Gilles, J R L

    2012-05-01

    The requirement for efficient mosquito mass rearing technology has been one of the major obstacles preventing the large scale application of the Sterile Insect Technique against mosquitoes. At the Food and Agriculture Organization/International Atomic Energy Agency (FAO/ IAEA) Insect Pest Control Laboratories we developed a larval rearing unit based on the use of a stainless steel rack that operates 50 thermoformed ABS plastic trays and is expected to be able to successfully rear 140,000-175,000 Anopheles arabiensis (Patton) adult mosquitoes per rack. The mechanized rearing unit is simple to handle, maintains minimal water temperature variation and negligible water evaporation and allows normal larval development. The mosquito mass-rearing tray was designed to provide a large surface area of shallow water that would closely mimic natural breeding sites. The trays stack into a dedicated rack structure and filling and draining were easily performed. The close stacking of the trays in the rack and the possibility to tightly line up several racks makes this rearing unit a valid solution for maximal use of the space thus reducing construction, heating, and cooling costs. The low amount of labor required to operate the system also reduces labor costs that represent one of the main expenditures in any mass rearing facility operation. Preliminary experiments performed on Aedes albopictus (Skuse) also confirm the possibility of successfully extending the use of this technology to other mosquito species. Our larval rearing unit could enhance any mosquito control strategy in which large-scale releases of mosquitoes are needed to suppress or replace natural populations. PMID:22679867

  16. Vehicle rear suspension mechanism

    SciTech Connect

    Kijima, T.; Maebayashi, J.

    1986-08-05

    A vehicle rear suspension mechanism is described which consists of: a suspension member connected with a vehicle body; wheel hub means supporting a rear wheel having a wheel center plane for rotation about a rotating axis; and connecting means for connecting the wheel hub means with the suspension member. The connecting means include ball joint means having a pivot center located forwardly of and below the rotating axis of the rear wheel and connecting the wheel hub means to the suspension member pivotably about the pivot center, first resilient means located between the wheel hub means and the suspension member rearwardly of and above the rotating axis of the rear wheel, and second resilient means located between the wheel hub means and the suspension member forwardly of and above the rotating axis of the rear wheel.

  17. Flow visualization techniques in the Airborne Laser Laboratory program

    NASA Technical Reports Server (NTRS)

    Walterick, R. E.; Vankuren, J. T.

    1980-01-01

    A turret/fairing assembly for laser applications was designed and tested. Wind tunnel testing was conducted using flow visualization techniques. The techniques used have included the methods of tufting, encapsulated liquid crystals, oil flow, sublimation and schlieren and shadowgraph photography. The results were directly applied to the design of fairing shapes for minimum drag and reduced turret buffet. In addition, the results are of primary importance to the study of light propagation paths in the near flow field of the turret cavity. Results indicate that the flow in the vicinity of the turret is an important factor for consideration in the design of suitable turret/fairing or aero-optic assemblies.

  18. Remote sensing and laboratory techniques for monitoring ocean dumping

    NASA Technical Reports Server (NTRS)

    Ohlhorst, C. W.; Johnson, R. W.; Meyer, E. R.

    1977-01-01

    Results of field experiments conducted in the Atlantic Coastal Zone indicate that plumes resulting from ocean dumping of acid waste and sewage sludge have distinguishable spectral characteristics when the radiance of the pollutant is normalized (ratioed to) background ocean water. Acid waste spectra peak between 550-650 nm while sewage sludge spectra have peak values at wavelengths of about 700 nm or greater. Results indicate that identification of acid waste and sewage sludge plumes may be independent of geographical location in the Atlantic Coastal Zone. Radiance ratio curves obtained in the laboratory qualitatively agree with those obtained from field experiments. Results from the July 25, 1977, Galveston Deep Ocean Dump Site experiment show the radiance ratio curve of the biodigested industrial waste to be fairly flat and similar to the radiance ratio curves of sewage sludge line dumps and sewage sludge spot dumps that have been in the water for several hours.

  19. Spawning and rearing Atlantic menhaden

    SciTech Connect

    Hettler, W.F.

    1981-04-01

    Two-year-old Atlantic menhaden (Brevoortia tyrannus) held in the laboratory at ambient temperatures and salinities for more than 1 year, were induced to spawn by injecting first human chorionic gonadotropin and then carp pituitary powder. Spawning took place at temperatures of 16 to 20/sup 0/C in a 2100-L indoor tank modified to recover the buoyant fertilized eggs. Larvae were reared to the juvenile stage on a diet of cultured rotifers (Brachionus plicatilus), sieved wild zooplankton (64 to 500 ..mu..m), brine shrimp (Artemia salina) nauplii, and powdered trout food.

  20. Novel Monitoring Techniques for Characterizing Frictional Interfaces in the Laboratory

    PubMed Central

    Selvadurai, Paul A.; Glaser, Steven D.

    2015-01-01

    A pressure-sensitive film was used to characterize the asperity contacts along a polymethyl methacrylate (PMMA) interface in the laboratory. The film has structural health monitoring (SHM) applications for flanges and other precision fittings and train rail condition monitoring. To calibrate the film, simple spherical indentation tests were performed and validated against a finite element model (FEM) to compare normal stress profiles. Experimental measurements of the normal stress profiles were within −7.7% to 6.6% of the numerical calculations between 12 and 50 MPa asperity normal stress. The film also possessed the capability of quantifying surface roughness, an important parameter when examining wear and attrition in SHM applications. A high definition video camera supplied data for photometric analysis (i.e., the measure of visible light) of asperities along the PMMA-PMMA interface in a direct shear configuration, taking advantage of the transparent nature of the sample material. Normal stress over individual asperities, calculated with the pressure-sensitive film, was compared to the light intensity transmitted through the interface. We found that the luminous intensity transmitted through individual asperities linearly increased 0.05643 ± 0.0012 candelas for an increase of 1 MPa in normal stress between normal stresses ranging from 23 to 33 MPa. PMID:25923930

  1. Fertility preservation techniques: laboratory and clinical progress and current issues.

    PubMed

    Anderson, R A

    2008-12-01

    Human fertility is dependent on maturation of germ cells through meiosis and their association with supporting cells, which in the female are also the source of sex steroids. These processes are sensitive to both chemotherapy and radiotherapy thus can be damaged by anti-cancer treatments. The uterus is also sensitive to radiotherapy. Our understanding of and the ability to manipulate fertility has increased together with survival rates from many cancers, particularly those affecting children, younger men, and women. The growth of interest in fertility preservation for cancer patients is a natural union of these two fields. Sperm banking has been available for many years, and is a recognized and evidence-based option for men that should be available to all. Options for women and pre-pubertal boys and girls are, however, more experimental, other than for women of committing oocytes to fertilization and cryopreservation as embryos. This Focus Issue of Reproduction aims to address the current status of some of the clinical and laboratory aspects of this burgeoning subspecialty to highlight not only areas of progress but also areas of uncertainty where future developments are required to allow the provision of accurate information, and safe and effective treatments. PMID:19074212

  2. Techniques to obtain orbital debris encounter speeds in the laboratory

    SciTech Connect

    Chhabildas, L.C.

    1996-06-01

    Understanding high-pressure material behavior is crucial to address the physical processes in hypervelocity impact events related to space sciences such as orbital-debris impact on a debris shield. At very high impact velocities, material properties will be dominated by phase changes, such as melting or vaporization, which cannot be achieved at lower impact velocities. Development of well-controlled, repeatable hypervelocity launch capabilities is the first step necessary to improve understanding of material behavior at extreme pressures and temperatures not currently available using conventional two-stage light-gas guns. In this paper, techniques used to extend the launch capabilities of a two-stage light gas gun to 16 km/s are described. It is anticipated that this technology will be useful in testing, evaluating, and design of various debris shields proposed for many different spacecrafts.

  3. Demonstrations of Extraterrestrial Life Detection Techniques in the High School Biology Laboratory

    ERIC Educational Resources Information Center

    Saltinski, Ronald

    1969-01-01

    Discusses the experimental procedures and equipment for exobiology projects at the high school level. An interdisciplinary approach involving electronic equipment and micro-biological laboratory techniques is used. Photographs and diagrams of equipment are included. Bibliography. (LC)

  4. Spatial repellency of transfluthrin-treated hessian strips against laboratory-reared Anopheles arabiensis mosquitoes in a semi-field tunnel cage

    PubMed Central

    2012-01-01

    Background Vapour phase spatial repellents deter mosquitoes from attacking one or more humans in a protected space. Simulation models indicate that high coverage of spatial repellents can enhance the impact of long - lasting insecticide nets (LLINs) and indoor residual spraying (IRS) where mosquito vectors commonly bite humans outdoors. Here we report a preliminary evaluation of an effective, user-friendly prototype product for delivering spatial repellents to protect against malaria vector mosquitoes. Findings Protective efficacy of a 4.0 0.3 m strip of hessian sacking treated with 10 ml of transfluthrin was evaluated in a 60 m 2 m 2.5 m netting tunnel with malaria-free insectary-reared Anopheles arabiensis Patton mosquitoes. Personal protection, in terms of proportional reduction of exposure to bites, was measured by comparing human landing catches of volunteers with treated and untreated strips. A freshly treated hessian strip reduced mosquito attack rate on human volunteers by > 99% and consistently conferred > 90% protective efficacy for a period of 6 months. Over the entire study period, only 22 out of 1400 released mosquitoes bit volunteers using the treated sacking strip while 894 out of 1400 mosquitoes released into cages containing volunteers using an untreated strip fed upon them. Conclusion Locally available natural fibers may be promising absorbent substrates for delivering spatial repellents, such as transfluthrin, to protect against mosquitoes in tropical settings. However, these observations relate to a single prototype specimen of this particular device, therefore, much more detailed, well replicated studies are essential to establish long-term efficacy, effectiveness, practicability and affordability. PMID:22433128

  5. A Survey of the Practices, Procedures, and Techniques in Undergraduate Organic Chemistry Teaching Laboratories

    ERIC Educational Resources Information Center

    Martin, Christopher B.; Schmidt, Monica; Soniat, Michael

    2011-01-01

    A survey was conducted of four-year institutions that teach undergraduate organic chemistry laboratories in the United States. The data include results from over 130 schools, describes the current practices at these institutions, and discusses the statistical results such as the scale of the laboratories performed, the chemical techniques applied,

  6. A Survey of the Practices, Procedures, and Techniques in Undergraduate Organic Chemistry Teaching Laboratories

    ERIC Educational Resources Information Center

    Martin, Christopher B.; Schmidt, Monica; Soniat, Michael

    2011-01-01

    A survey was conducted of four-year institutions that teach undergraduate organic chemistry laboratories in the United States. The data include results from over 130 schools, describes the current practices at these institutions, and discusses the statistical results such as the scale of the laboratories performed, the chemical techniques applied,…

  7. Basic Laboratory Techniques for Students of Biology and Small Animal Care.

    ERIC Educational Resources Information Center

    Schoen, Jerome; Berman, Paul

    This document provides descriptions of lessons, activities, and laboratory experiments to be used in a course on basic laboratory techniques for students in biology and small animal care. These learning experiences are designed to be completed during one class period daily for approximately 70 days per semester. (It is assumed that this would…

  8. Costly Nutritious Diets do not Necessarily Translate into Better Performance of Artificially Reared Fruit Flies (Diptera: Tephritidae)

    PubMed Central

    Pascacio-Villafán, C.; Williams, T.; Sivinski, J.; Birke, A.; Aluja, M.

    2015-01-01

    Protein, lipid, carbohydrate, and energy contents of three artificial diets (Xal2, Met1, and Met2) used for laboratory-rearing and mass-rearing the Mexican fruit fly, Anastrepha ludens (Loew), for a sterile insect technique program were measured. The larval survival, pupation, pupal weight, adult emergence, sex ratio, and flight capacity of the flies reared on each of these diets were also quantified. The diet with the highest nutrient and energy content was Xal2 followed by Met2 and Met1, but larval recovery and percent pupation was significantly higher in flies reared on either the Met1 or Met2 diets. A. ludens reared on Xal2 exhibited the highest proportion of adults capable of flight. No other response variable differed significantly among the three diets tested. This suggests that a high content of nutrients and multiple sources of protein (dried yeast and wheat germ in the case of the Xal2 diet) do not necessarily improve overall performance or fly quality. We conclude that nutritious diets for A. ludens can be modified to reduce their cost without compromising the performance of artificially reared flies. PMID:26470103

  9. Costly Nutritious Diets do not Necessarily Translate into Better Performance of Artificially Reared Fruit Flies (Diptera: Tephritidae).

    PubMed

    Pascacio-Villafán, C; Williams, T; Sivinski, J; Birke, A; Aluja, M

    2015-02-01

    Protein, lipid, carbohydrate, and energy contents of three artificial diets (Xal2, Met1, and Met2) used for laboratory-rearing and mass-rearing the Mexican fruit fly, Anastrepha ludens (Loew), for a sterile insect technique program were measured. The larval survival, pupation, pupal weight, adult emergence, sex ratio, and flight capacity of the flies reared on each of these diets were also quantified. The diet with the highest nutrient and energy content was Xal2 followed by Met2 and Met1, but larval recovery and percent pupation was significantly higher in flies reared on either the Met1 or Met2 diets. A. ludens reared on Xal2 exhibited the highest proportion of adults capable of flight. No other response variable differed significantly among the three diets tested. This suggests that a high content of nutrients and multiple sources of protein (dried yeast and wheat germ in the case of the Xal2 diet) do not necessarily improve overall performance or fly quality. We conclude that nutritious diets for A. ludens can be modified to reduce their cost without compromising the performance of artificially reared flies. PMID:26470103

  10. PROJECT SUCCESS: Marine Science. (Introductory Packet, Basic Marine Science Laboratory Techniques, Oceanographic Instruments, Individual Projects, Bibliography).

    ERIC Educational Resources Information Center

    Demaray, Bryan

    Five packets comprise the marine science component of an enrichment program for gifted elementary students. Considered in the introductory section are identification (pre/post measure) procedures. Remaining packets address the following topics (subtopics in parentheses): basic marine science laboratory techniques (microscope techniques and metric…

  11. The Effectiveness of Active and Traditional Teaching Techniques in the Orthopedic Assessment Laboratory

    ERIC Educational Resources Information Center

    Nottingham, Sara; Verscheure, Susan

    2010-01-01

    Active learning is a teaching methodology with a focus on student-centered learning that engages students in the educational process. This study implemented active learning techniques in an orthopedic assessment laboratory, and the effects of these teaching techniques. Mean scores from written exams, practical exams, and final course evaluations…

  12. CONSUMPTIONS RATES OF SUMMER FLOUNDER LARVAE ON ROTIFER AND BRINE SHRIMP PREY DURING LARVAL REARING

    EPA Science Inventory

    Larval summer flounder Paralichthys dentatus were hatched and reared through metamorphosis in the laboratory. At several points in the rearing cycle, larvae were removed from their rearing chambers and placed in small bowls, where they were fed known quantities of the rotifer Bra...

  13. NORTH REAR AND WEST SIDE, Looking southeast down Saturn Boulevard. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    NORTH REAR AND WEST SIDE, Looking southeast down Saturn Boulevard. February, 1998 - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Electrical Substation, Test Area 1-115, northwest end of Saturn Boulevard, Boron, Kern County, CA

  14. 22. DETAIL, TWO LIGHTING TYPES AT REAR OF TEST STAND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. DETAIL, TWO LIGHTING TYPES AT REAR OF TEST STAND 1-A. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Test Stand 1-A, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA

  15. 8. SOUTH REAR, SUPERSTRUCTURE. Looking north from deck. Edwards ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. SOUTH REAR, SUPERSTRUCTURE. Looking north from deck. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Test Stand 1-A, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA

  16. 22. LOOKING NORTHEAST FROM RECEIVING PLATFORM AT THE REAR (EAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. LOOKING NORTHEAST FROM RECEIVING PLATFORM AT THE REAR (EAST SIDE) OF BUILDING, SHOWING SOUTH SIDE OF NORTH WING AND SOUTH SIDE OF FOOD PRESERVATION AND SANITATION LABORATORY (Harms) - Dairy Industry Building, Iowa State University campus, Ames, Story County, IA

  17. 14. REAR (EAST SIDE) OF BUILDING SHOWING RECEIVING COURT AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. REAR (EAST SIDE) OF BUILDING SHOWING RECEIVING COURT AND SOUTH SIDE OF FOOD PRESERVATION AND SANITATION LABORATORY, LOOKING WEST-NORTHWEST (Harms) - Dairy Industry Building, Iowa State University campus, Ames, Story County, IA

  18. Northeast (rear) and northwest (side) elevations, view to southwest ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Northeast (rear) and northwest (side) elevations, view to southwest - Bureau of Mines Metallurgical Research Laboratory, Original Building, Date Street north of U.S. Highway 93, Boulder City, Clark County, NV

  19. Southeast (side) and northeast (rear) elevations, view to northwest ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Southeast (side) and northeast (rear) elevations, view to northwest - Bureau of Mines Metallurgical Research Laboratory, Original Building, Date Street north of U.S. Highway 93, Boulder City, Clark County, NV

  20. The K.U. Leuven pulsed field laboratory: magnet technology and measuring techniques

    NASA Astrophysics Data System (ADS)

    Herlach, Fritz

    2004-04-01

    The pulsed field laboratory at the Katholieke Universiteit Leuven is the oldest surviving magnet laboratory in Europe. Several innovations in magnet technology have been pioneered at this laboratory. It was and is engaged in fruitful co-operation with many other pulsed field laboratories worldwide, and as a partner of the European ARMS project it develops advanced insert coils for fields up to 80 T. The laboratory has a magnet testing station and seven measuring stations up to 70 T; up to four of these can be used in parallel. Measuring techniques include magneto-transport, magnetisation, infrared resonances and photoluminescence. Principal research topics are high temperature superconductors and quantum dots. The laboratory has established close co-operation with the LNCMP at Toulouse; in the context of large European facilities it will have the status of a satellite user facility.

  1. 9. NORTH FLANK AND (WEST) REAR WITH REAR OF ADMINISTRATION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. NORTH FLANK AND (WEST) REAR WITH REAR OF ADMINISTRATION BUILDING, LOOKING SOUTHEAST ACROSS GARDEN FROM TOWER GROVE SOUTH PORCH - Missouri Botanical Garden, Henry Shaw Townhouse, 2345 Tower Grove Avenue, Saint Louis, Independent City, MO

  2. 43. FIRST FLOOR, REAR HALL: REAR HALLWAY BEHIND CURVED STAIRS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    43. FIRST FLOOR, REAR HALL: REAR HALLWAY BEHIND CURVED STAIRS LOOKING NORTH TO 19TH CENTURY ADDITION. Arch defines north wall of original house. - George A. Trenholm Mansion, 172 Rutledge Avenue, Charleston, Charleston County, SC

  3. Toward Communal Child Rearing

    ERIC Educational Resources Information Center

    Sands, Rosalind M.

    1973-01-01

    Social work's preoccupation with the preservation of the nuclear family has blinded it to the necessity of finding new ways to care for children. This myopia has impeded recognition of the forces in American life that are bringing social change and new forms of child rearing. This article describes some of these phenomena and concludes that…

  4. ANTERIOR CRUCIATE LIGAMENT RECONSTRUCTION USING THE DOUBLE-BUNDLE TECHNIQUE – EVALUATION IN THE BIOMECHANICS LABORATORY

    PubMed Central

    D'Elia, Caio Oliveira; Bitar, Alexandre Carneiro; Castropil, Wagner; Garofo, Antônio Guilherme Padovani; Cantuária, Anita Lopes; Orselli, Maria Isabel Veras; Luques, Isabela Ugo; Duarte, Marcos

    2015-01-01

    Objective: The objective of this study was to describe the methodology of knee rotation analysis using biomechanics laboratory instruments and to present the preliminary results from a comparative study on patients who underwent anterior cruciate ligament (ACL) reconstruction using the double-bundle technique. Methods: The protocol currently used in our laboratory was described. Three-dimensional kinematic analysis was performed and knee rotation amplitude was measured on eight normal patients (control group) and 12 patients who were operated using the double-bundle technique, by means of three tasks in the biomechanics laboratory. Results: No significant differences between operated and non-operated sides were shown in relation to the mean amplitudes of gait, gait with change in direction or gait with change in direction when going down stairs (p > 0.13). Conclusion: The preliminary results did not show any difference in the double-bundle ACL reconstruction technique in relation to the contralateral side and the control group. PMID:27027003

  5. Mass rearing history and irradiation affect mating performance of the male fruit fly, Anastrepha obliqua.

    PubMed

    Rull, Juan; Encarnación, Nery; Birke, Andrea

    2012-01-01

    As an initial step to improve the efficiency of the sterile insect technique applied to eradicate, suppress, and control wild Anastrepha obliqua (Macquart) (Diptera: Tephritidae) in mango producing areas of Mexico, the effect of radiation dose and mass rearing history on male mating performance was examined. Field cage tests in which both male and female laboratory flies were irradiated at different doses (0, 40, and 80 Gy) were released with cohorts of wild flies of both sexes, revealing that both mass rearing history and irradiation affected male mating performance. Laboratory males were accepted for copulation by wild females less frequently than wild males. Copulations involving laboratory males were shorter than those involving wild males. Irradiated males mated less frequently with wild females than wild males, and irradiated females appeared to be less able to reject courting males of both origins. High levels of fertility for untreated laboratory females crossed with males irradiated at different doses may reflect problems in mass rearing affecting homogeneity of pupal age before irradiation, and possibly masked a dose effect. Proposed remedial measures to improve male mating performance are discussed. PMID:22957485

  6. Assessment of fracture-sampling techniques for laboratory tests on core

    USGS Publications Warehouse

    Severson, G.R.; Boernge, J.M.

    1991-01-01

    As part of the site characterization work to be done at Yucca Mountain in Nye County, Nevada, a candidate site for the first mined-geologic repository for high-level nuclear waste, laboratory tests are proposed to evaluate fluid flow in single fractures. Laboratory and onsite tests were conducted to develop methods for collecting rock-core samples containing single fractures for the subsequent laboratory tests. Techniques for collecting rock cores with axial (parallel to the core axis) and radial (perpendicular to the core axis) fractures are discussed.

  7. Rearing environment affects behaviour of jumping spiders.

    PubMed

    Carducci; Jakob

    2000-01-01

    We tested the effect of rearing conditions on the behaviour of jumping spiders, Phidippus audax. Spiders were assigned randomly to either small or large cages that either were empty or contained a painted dowel. Laboratory-reared spiders were raised from second instar to adult in these environments. Field-caught adults also were randomly assigned to these containers and were held for approximately 4 months prior to testing. We presented spiders with three tests designed to examine a range of behaviours. Field-caught spiders were more likely than laboratory-reared spiders to (1) react to videotaped prey, (2) progress further on a detour test, and (3) be less stereotactic and more active in an open field. Larger cage size and the presence of the dowel also improved performance in several tests. Our results suggest that the rearing conditions we used, which are commonly employed by behavioural researchers, may profoundly influence the behaviour of adult spiders. Copyright 2000 The Association for the Study of Animal Behaviour. PMID:10640365

  8. Sequence variability in internal transcribed spacers of nuclear ribosomal DNA among isolates of the oxyurid nematodes Syphacia obvelata and Aspiculuris tetraptera from mice reared in laboratories in China.

    PubMed

    Qiu, J H; Lou, Y; Zhang, Y; Chang, Q C; Liu, Z X; Duan, H; Guo, D H; Gao, D Z; Yue, D M; Wang, C R

    2016-01-01

    This study examined sequence variability in internal transcribed spacers (ITS) of nuclear ribosomal DNA among Syphacia obvelata and Aspiculuris tetraptera isolates from laboratory mice from different geographical locations in China. ITS1, 5.8S and ITS2 rDNA were amplified separately from adult S. obvelata and A. tetraptera individuals by polymerase chain reaction (PCR), and the amplicons were subjected to sequencing from both directions. The lengths of the sequences of ITS1, 5.8S and ITS2 rDNA from both nematodes were 314 bp and 456 bp, 157 bp, and 273 bp and 419 bp, respectively. The intraspecific sequence variations in S. obvelata ITS1 were 0-0.3%. For A. tetraptera they were 0-0.7% in ITS1 and 0-1.0% in ITS2. However, the interspecific sequence differences among members of the infraorder Oxyuridomorpha were significantly higher, being 54.0-65.5% for ITS1 and 55.3-64.1% for ITS2. Phylogenetic analysis based on the combined partial sequences of ITS1 and ITS2 using three inference methods - Bayesian inference, maximum likelihood and maximum parsimony - revealed that all the S. obvelata and A. tetraptera samples formed independent monophyletic groups. Syphacia obvelata was closer to Syphacia muris than to A. tetraptera, consistent with morphological classification. These results demonstrate that ITS1 and ITS2 rDNA sequences are useful markers for population genetic studies of oxyurid nematodes. PMID:26693888

  9. Application of the One-Minute Preceptor Technique by Novice Teachers in the Gross Anatomy Laboratory

    ERIC Educational Resources Information Center

    Chan, Lap Ki; Yang, Jian; Irby, David M.

    2015-01-01

    The one-minute preceptor (OMP) was originally developed in the ambulatory care setting as a time-efficient teaching technique for learner-centered clinical training. There are also possible advantages of using the OMP in the gross anatomy laboratory. However, in a previous study it was found that providing training to experienced gross anatomy

  10. TECHNIQUES OF TAPE PREPARATION AND DUPLICATION, WITH SUGGESTIONS FOR A LANGUAGE LABORATORY.

    ERIC Educational Resources Information Center

    Kansas State Dept. of Public Instruction, Topeka.

    PART ONE OF THIS BULLETIN PROVIDES HELP IN THE TWO CRITICAL AREAS OF MASTER TAPE PREPARATION AND DUPLICATION. SUPPLEMENTED BY NUMEROUS PHOTOGRAPHS AND DIAGRAMS OF EQUIPMENT AND DUPLICATION TECHNIQUES, THE BULLETIN DESCRIBES MASTER PROGRAM DUPLICATION USING LANGUAGE LABORATORY EQUIPMENT, A PROFESSIONAL MASS DUPLICATOR, A TAPE RECORDER, A RECORD…

  11. DEVELOPMENT OF TECHNIQUES AND METHODOLOGY FOR THE LABORATORY CULTURE OF STRIPED BASS, 'MORONE SAXATILIS' (WALBAUM)

    EPA Science Inventory

    This research was undertaken to develop laboratory culture techniques for striped bass (Morone saxatilis) that could be used to provide an adequate supply of various life stages of this important fish species for water quality and hazard evaluation testing. The work includes both...

  12. An Enzymatic Clinical Chemistry Laboratory Experiment Incorporating an Introduction to Mathematical Method Comparison Techniques

    ERIC Educational Resources Information Center

    Duxbury, Mark

    2004-01-01

    An enzymatic laboratory experiment based on the analysis of serum is described that is suitable for students of clinical chemistry. The experiment incorporates an introduction to mathematical method-comparison techniques in which three different clinical glucose analysis methods are compared using linear regression and Bland-Altman difference…

  13. DEVELOPMENT OF TECHNIQUES AND METHODOLOGY FOR THE LABORATORY CULTURE OF STRIPED BASS, MORONE SAXATILIS (WALBAUM)

    EPA Science Inventory

    This report describes the research undertaken to develop laboratory culture techniques for striped bass (Morone saxatilis) which can be used to provide an adequate supply of various life stages of this important fish species for water quality and hazard evaluation testing. For ea...

  14. Application of the One-Minute Preceptor Technique by Novice Teachers in the Gross Anatomy Laboratory

    ERIC Educational Resources Information Center

    Chan, Lap Ki; Yang, Jian; Irby, David M.

    2015-01-01

    The one-minute preceptor (OMP) was originally developed in the ambulatory care setting as a time-efficient teaching technique for learner-centered clinical training. There are also possible advantages of using the OMP in the gross anatomy laboratory. However, in a previous study it was found that providing training to experienced gross anatomy…

  15. Rear leit observation

    NASA Astrophysics Data System (ADS)

    Dawson, P.; Ferguson, A. J. L.; Walmsley, D. G.

    1982-11-01

    We have observed light emission from the rear of CaF 2-roughened Al-I-Au tunnel sandwiches. Like the forward emitted light, its spectral intensity shows a sharp drop at energies > 2.5 eV. We interpret the results in terms of both emissions being mediated by a common surface or interface plasmon; the plasmon is damped above 2.5 eV by excitation of an interband transition in Au.

  16. Rearing methods for the black soldier fly (Diptera: Stratiomyidae).

    PubMed

    Sheppard, D Craig; Tomberlin, Jeffery K; Joyce, John A; Kiser, Barbara C; Sumner, Sonya M

    2002-07-01

    The black soldier fly, Heretia illucens (L.), is a nonpest tropical and warm-temperate region insect that is useful for managing large concentrations of animal manure and other biosolids. Manure management relying on wild fly oviposition has been successful in several studies. However, confidence in this robust natural system was low and biological studies were hampered by the lack of a dependable source of eggs and larvae. Larvae had been reared easily by earlier investigators, but achieving mating had been problematic. We achieved mating reliably in a 2 by 2 by 4-m screen cage in a 7 by 9 by 5-m greenhouse where sunlight and adequate space for aerial mating were available. Mating occurred during the shortest days of winter if the sun was not obscured by clouds. Adults were provided with water, but no food was required. Techniques for egg collection and larval rearing are given. Larvae were fed a moist mixture of wheat bran, corn meal, and alfalfa meal. This culture has been maintained for 3 yr. Maintainance of a black soldier fly laboratory colony will allow for development of manure management systems in fully enclosed animal housing and in colder regions. PMID:12144307

  17. Effects of implant angulation, material selection, and impression technique on impression accuracy: a preliminary laboratory study.

    PubMed

    Rutkunas, Vygandas; Sveikata, Kestutis; Savickas, Raimondas

    2012-01-01

    The aim of this preliminary laboratory study was to evaluate the effects of 5- and 25-degree implant angulations in simulated clinical casts on an impression's accuracy when using different impression materials and tray selections. A convenience sample of each implant angulation group was selected for both open and closed trays in combination with one polyether and two polyvinyl siloxane impression materials. The influence of material and technique appeared to be significant for both 5- and 25-degree angulations (P < .05), and increased angulation tended to decrease impression accuracy. The open-tray technique was more accurate with highly nonaxially oriented implants for the small sample size investigated. PMID:22930776

  18. Applications of nuclear techniques for in vivo body composition studies at Brookhaven National Laboratory

    SciTech Connect

    Cohn, S.H.; Ellis, K.J.; Vartsky, D.; Vaswani, A.N.; Wielopolski, L.

    1981-01-01

    A series of technical developments and their clinical applications in various nuclear technologies at Brookhaven National Laboratory is described. These include the development of a portable neutron activation facility for measuring cadmium in vivo in kidney and liver, a technique for the measurement of body iron utilizing nuclear resonant scattering of gamma rays, a non-invasive measure of the skeletal levels of lead by an x-ray fluorescence technique, and the development of a pulsed Van de Graaff generator as a source of pulsed neutrons for the measurement of lung silicon. (ACR)

  19. Ultimate ceramic veneer: a laboratory-guided preparation technique for minimally invasive laminate veneers.

    PubMed

    De Andrade, Oswaldo Scopin; Hirata, Ronaldo; Celestrino, Marcos; Seto, Marcio; Siqueira, Sérgio; Nahas, Rodrigo

    2012-06-01

    Clinical success of ceramic laminate veneers depends on material selection, bonding procedures, controlled laboratory steps, and enamel preservation. Enamel preservation is the most critical because excessive tooth preparation can expose dentin reducing bond strength, which is a factor that can cause a decrease in long-term clinical success. The proposed technique based on carefully treatment planning developed between clinician and dental technician helps to maximize enamel preservation, which is an important element for clinical success. PMID:22856034

  20. An artificial diet for rearing Cochliomyia macellaria (Diptera: Calliphoridae).

    PubMed

    Chaudhury, M F; Skoda, S R

    2013-08-01

    Larvae of the secondary screwworm, Cochliomyia macellaria (F.), feed on carrion and may sometimes cause animal myiasis. They have been reared in the laboratory on various animal tissues to study their growth and development because of their importance in forensic science. We use secondary screwworms in our laboratory for preliminary experiments as a model for the primary screwworm, Cochliomyia hominivorax (Coquerel), which has been eradicated from the United States. C. macellaria larvae reared on animal tissues produced a putrid odor, an unfavorable condition in the laboratory, and variable pupal size and weight, bringing into question the validity of use as a model for C. hominivorax. Therefore, studies were conducted to develop a less odiferous artificial diet with reduced variability in resulting pupae by comparing three diets: 1) an artificial diet prepared from spray-dried blood, spray-dried poultry egg, dry milk substitute, and solidified with a polyacrylate polymer gel; 2) a fresh blood-based diet prepared similarly, except fresh bovine blood was used in place of spray-dried blood; and 3) a beef liver diet. Data from seven life-history parameters of resulting insects were collected and analyzed. Larval and pupal weights of C. macellaria reared on both the dry and fresh blood-based diets were significantly higher than those reared on the liver diet. Numbers of pupae and percentage of adult emergence were also significantly higher from both dry blood and fresh blood-based diets than those reared on the liver diet. Female flies developing from larvae reared on dry and fresh blood-based diets laid significantly more eggs than females developing from larvae reared on the liver diet. Results show that C. macellaria larvae developed and grew normally in the dry and fresh blood-based diets, indicating that an artificial diet can effectively replace the liver diet commonly used for rearing C. macellaria. PMID:24020312

  1. 37. WEST REAR OF POWERHOUSE AND CAR BARN: West rear ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    37. WEST REAR OF POWERHOUSE AND CAR BARN: West rear of powerhouse and car barn, showing the turntable and tracks used to move cars in and out of the building's repair and storage area. - San Francisco Cable Railway, Washington & Mason Streets, San Francisco, San Francisco County, CA

  2. VIEW OF SHADED REAR YARDS AND TERRACING, SHOWING REAR OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF SHADED REAR YARDS AND TERRACING, SHOWING REAR OF 527 BIRCH CIRCLE ON LEFT. VIEW FACING NORTHEAST - Camp H.M. Smith and Navy Public Works Center Manana Title VII (Capehart) Housing, Intersection of Acacia Road and Brich Circle, Pearl City, Honolulu County, HI

  3. Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory.

    PubMed Central

    Ieven, M; Goossens, H

    1997-01-01

    Clinical laboratories are increasingly receiving requests to perform nucleic acid amplification tests for the detection of a wide variety of infectious agents. In this paper, the efficiency of nucleic acid amplification techniques for the diagnosis of respiratory tract infections is reviewed. In general, these techniques should be applied only for the detection of microorganisms for which available diagnostic techniques are markedly insensitive or nonexistent or when turnaround times for existing tests (e.g., viral culture) are much longer than those expected with amplification. This is the case for rhinoviruses, coronaviruses, and hantaviruses causing a pulmonary syndrome, Bordetella pertussis, Chlamydia pneumoniae, Mycoplasma pneumoniae, and Coxiella burnetii. For Legionella spp. and fungi, contamination originating from the environment is a limiting factor in interpretation of results, as is the difficulty in differentiating colonization and infection. Detection of these agents in urine or blood by amplification techniques remains to be evaluated. In the clinical setting, there is no need for molecular diagnostic tests for the diagnosis of Pneumocystis carinii. At present, amplification methods for Mycobacterium tuberculosis cannot replace the classical diagnostic techniques, due to their lack of sensitivity and the absence of specific internal controls for the detection of inhibitors of the reaction. Also, the results of interlaboratory comparisons are unsatisfactory. Furthermore, isolates are needed for susceptibility studies. Additional work remains to be done on sample preparation methods, comparison between different amplification methods, and analysis of results. The techniques can be useful for the rapid identification of M. tuberculosis in particular circumstances, as well as the rapid detection of most rifampin-resistant isolates. The introduction of diagnostic amplification techniques into a clinical laboratory implies a level of proficiency for excluding false-positive and false-negative results. PMID:9105753

  4. A Molecular Method to Discriminate between Mass-Reared Sterile and Wild Tsetse Flies during Eradication Programmes That Have a Sterile Insect Technique Component

    PubMed Central

    Pagabeleguem, Soumaïla; Gimonneau, Geoffrey; Seck, Momar Talla; Vreysen, Marc J. B.; Sall, Baba; Rayaissé, Jean-Baptiste; Sidibé, Issa; Bouyer, Jérémy; Ravel, Sophie

    2016-01-01

    Background The Government of Senegal has embarked several years ago on a project that aims to eradicate Glossina palpalis gambiensis from the Niayes area. The removal of the animal trypanosomosis would allow the development more efficient livestock production systems. The project was implemented using an area-wide integrated pest management strategy including a sterile insect technique (SIT) component. The released sterile male flies originated from a colony from Burkina Faso. Methodology/Principal Findings Monitoring the efficacy of the sterile male releases requires the discrimination between wild and sterile male G. p. gambiensis that are sampled in monitoring traps. Before being released, sterile male flies were marked with a fluorescent dye powder. The marking was however not infallible with some sterile flies only slightly marked or some wild flies contaminated with a few dye particles in the monitoring traps. Trapped flies can also be damaged due to predation by ants, making it difficult to discriminate between wild and sterile males using a fluorescence camera and / or a fluorescence microscope. We developed a molecular technique based on the determination of cytochrome oxidase haplotypes of G. p. gambiensis to discriminate between wild and sterile males. DNA was isolated from the head of flies and a portion of the 5’ end of the mitochondrial gene cytochrome oxidase I was amplified to be finally sequenced. Our results indicated that all the sterile males from the Burkina Faso colony displayed the same haplotype and systematically differed from wild male flies trapped in Senegal and Burkina Faso. This allowed 100% discrimination between sterile and wild male G. p. gambiensis. Conclusions/Significance This tool might be useful for other tsetse control campaigns with a SIT component in the framework of the Pan-African Tsetse and Trypanosomosis Eradication Campaign (PATTEC) and, more generally, for other vector or insect pest control programs. PMID:26901049

  5. 29. NORTH SIDE AND WEST REAR OF CAR BARN DURING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    29. NORTH SIDE AND WEST REAR OF CAR BARN DURING RECONSTRUCTION: Photocopy of May 1908 photograph showing the north side and west rear of powerhouse and car barn. The windows on the north wall of the building were later bricked up. Note the wooden roof trusses of the main building, and the different construction techniques used in rebuilding the 'annex,' closest to the viewer. - San Francisco Cable Railway, Washington & Mason Streets, San Francisco, San Francisco County, CA

  6. Laboratory analyses of micron-sized solid grains: Experimental techniques and recent results

    NASA Technical Reports Server (NTRS)

    Colangeli, L.; Bussoletti, E.; Blanco, A.; Borghesi, A.; Fonti, S.; Orofino, V.; Schwehm, G.

    1989-01-01

    Morphological and spectrophotometric investigations have been extensively applied in the past years to various kinds of micron and/or submicron-sized grains formed by materials which are candidate to be present in space. The samples are produced in the laboratory and then characterized in their physio-chemical properties. Some of the most recent results obtained on various kinds of carbonaceous materials are reported. Main attention is devoted to spectroscopic results in the VUV and IR wavelength ranges, where many of the analyzed samples show typical fingerprints which can be identified also in astrophysical and cometary materials. The laboratory methodologies used so far are also critically discussed in order to point out capabilities and present limitations, in the view of possible application to returned comet samples. Suggestions are given to develop new techniques which should overcome some of the problems faced in the manipulation and analysis of micron solid samples.

  7. Effects of rearing treatment on the behavior of captive whooping cranes (Grus americana)

    USGS Publications Warehouse

    Kreger, M.D.; Estevez, I.; Hatfield, J.S.; Gee, G.F.

    2004-01-01

    Small founder populations of whooping cranes are managed to maximize egg production for the purpose of reintroducing young to the wild. This results in an excessive number of hatched chicks that cannot be naturally reared by parents. Hand-rearing techniques have been developed to raise the additional hatches. However, hand rearing may affect the behavior of the birds and their chances of survival later in life. The objectives of this study were to determine the impact of rearing practices on the behavior of whooping crane chicks. The birds were reared under three commonly used rearing techniques: parent reared (PR), hand reared (HR), and hand reared with exercise (HRE). Fifty-six whooping crane chicks were observed by focal animal sampling from hatch to 20 weeks of age. During these observations, occurrences of comfort behavior, aggression, foraging, nonvigilance, sleep, vigilance, and other types of behavior were collected. Data were analyzed using mixed models repeated measures analysis of variance (ANOVA). Behavior was affected by rearing treatment, age, and time of day. PR birds spent more time being vigilant than HR and HRE birds. An inverse correlation was found between percentage of time foraging and vigilant (r = -0.686, P < 0.0001). However, there were no differences in the behavior of birds reared in HR or HRE programs.

  8. Complementary and Emerging Techniques for Astrophysical Ices Processed in the Laboratory

    NASA Astrophysics Data System (ADS)

    Allodi, M. A.; Baragiola, R. A.; Baratta, G. A.; Barucci, M. A.; Blake, G. A.; Boduch, P.; Brucato, J. R.; Contreras, C.; Cuylle, S. H.; Fulvio, D.; Gudipati, M. S.; Ioppolo, S.; Kaňuchová, Z.; Lignell, A.; Linnartz, H.; Palumbo, M. E.; Raut, U.; Rothard, H.; Salama, F.; Savchenko, E. V.; Sciamma-O'Brien, E.; Strazzulla, G.

    2013-12-01

    Inter- and circumstellar ices comprise different molecules accreted on cold dust particles. These icy dust grains provide a molecule reservoir where particles can interact and react. As the grain acts as a third body, capable of absorbing energy, icy surfaces in space have a catalytic effect. Chemical reactions are triggered by a number of possible processes; (i) irradiation by light, typically UV photons from the interstellar radiation field and Ly- α radiation emitted by excited hydrogen, but also X-rays, (ii) bombardment by particles, free atoms (most noticeably hydrogen, but also N, C, O and D-atoms), electrons, low energy ions and cosmic rays, and (iii) thermal processing. All these effects cause ices to (photo)desorb, induce fragmentation or ionization in the ice, and eventual recombination will make molecules to react and to form more and more complex species. The effects of this solid state astrochemistry are observed by astronomers; nearly 180 different molecules (not including isotopologues) have been unambiguously identified in the inter- and circumstellar medium, and the abundances of a substantial part of these species cannot be explained by gas phase reaction schemes only and must involve solid state chemistry. Icy dust grains in space experience different chemical stages. In the diffuse medium grains are barely covered by molecules, but upon gravitational collapse and darkening of the cloud, temperatures drop and dust grains start acting as micrometer sized cryopumps. More and more species accrete, until even the most volatile species are frozen. In parallel (non)energetic processing can take place, particularly during planet and star formation when radiation and particle fluxes are intense. The physical and chemical properties of ice clearly provide a snapshotroot to characterize the cosmological chemical evolution. In order to fully interpret the astronomical observations, therefore, dedicated laboratory experiments are needed that simulate dust grain formation and processing as well as ice mantle chemistry under astronomical conditions and in full control of the relevant parameters; ice morphology (i.e., structure), composition, temperature, UV and particle fluxes, etc., yielding parameters that can be used for astrochemical modeling and for comparison with the observations. This is the topic of the present manuscript. Laboratory experiments simulating the conditions in space are conducted for decades all over the world, but particularly in recent years new techniques have made it possible to study reactions involving inter- and circumstellar dust and ice analogues at an unprecedented level of detail. Whereas in the past "top-down scenarios" allowed to conclude on the importance of the solid state for the chemical enrichment of space, presently "bottom-up approaches" make it possible to fully quantify the involved reactions, and to provide information on processes at the molecular level. The recent progress in the field of "solid state laboratory astrophysics" is a consequence of the use of ultra high vacuum systems, of new radiation sources, such as synchrotrons and laser systems that allow extensions to wavelength domains that long have not been accessible, including the THz domain, and the use of highly sensitive gas phase detection techniques, explicitly applied to characterize the solid state such as fluorescence, luminescence, cavity ring-down spectroscopy and sophisticated mass spectrometric techniques. This paper presents an overview of the techniques being used in astrochemical laboratories worldwide, but it is incomplete in the sense that it summarizes the outcome of a 3-day workshop of the authors in November 2012 (at the Observatoire de Meudon in France), with several laboratories represented, but not all. The paper references earlier work, but it is incomplete with regard to latest developments of techniques used in laboratories not represented at the workshop.

  9. Murillo's paintings revealed by spectroscopic techniques and dedicated laboratory-made micro X-ray diffraction.

    PubMed

    Duran, A; Siguenza, M B; Franquelo, M L; Jimenez de Haro, M C; Justo, A; Perez-Rodriguez, J L

    2010-06-25

    This paper describes one of the first case studies using micro-diffraction laboratory-made systems to analyse painting cross-sections. Pigments, such as lead white, vermilion, red ochre, red lac, lapis lazuli, smalt, lead tin yellow type I, massicot, ivory black, lamp black and malachite, were detected in cross-sections prepared from six Bartolomé Esteban Murillo paintings by micro-Raman and micro-XRD combined with complementary techniques (optical microscopy, SEM-EDS, and FT-IR). The use of micro-XRD was necessary due to the poor results obtained with conventional XRD. In some cases, pigment identification was only possible by combining results from the different analytical techniques utilised in this study. PMID:20541637

  10. A Laboratory Course for Teaching Laboratory Techniques, Experimental Design, Statistical Analysis, and Peer Review Process to Undergraduate Science Students

    ERIC Educational Resources Information Center

    Gliddon, C. M.; Rosengren, R. J.

    2012-01-01

    This article describes a 13-week laboratory course called Human Toxicology taught at the University of Otago, New Zealand. This course used a guided inquiry based laboratory coupled with formative assessment and collaborative learning to develop in undergraduate students the skills of problem solving/critical thinking, data interpretation and

  11. A Laboratory Course for Teaching Laboratory Techniques, Experimental Design, Statistical Analysis, and Peer Review Process to Undergraduate Science Students

    ERIC Educational Resources Information Center

    Gliddon, C. M.; Rosengren, R. J.

    2012-01-01

    This article describes a 13-week laboratory course called Human Toxicology taught at the University of Otago, New Zealand. This course used a guided inquiry based laboratory coupled with formative assessment and collaborative learning to develop in undergraduate students the skills of problem solving/critical thinking, data interpretation and…

  12. 6. HOUSE REAR EXTERIOR SHOWING UTILITY PORCH AND REAR ENTRANCE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. HOUSE REAR EXTERIOR SHOWING UTILITY PORCH AND REAR ENTRANCE TO DINNING/KITCHEN AREA AT PHOTO CENTER. SMALL RECTANGULAR STRUCTURE ATTACHED TO SIDE OF HOUSE AT PHOTO RIGHT CENTER BELOW SMALL, DOUBLEHUNG, WOOD-FRAME WINDOW IS THE HOT WATER HEATER CABINET. VIEW TO SOUTHEAST. - Big Creek Hydroelectric System, Big Creek Town, Operator House, Orchard Avenue south of Huntington Lake Road, Big Creek, Fresno County, CA

  13. New developments of X-ray fluorescence imaging techniques in laboratory

    NASA Astrophysics Data System (ADS)

    Tsuji, Kouichi; Matsuno, Tsuyoshi; Takimoto, Yuki; Yamanashi, Masaki; Kometani, Noritsugu; Sasaki, Yuji C.; Hasegawa, Takeshi; Kato, Shuichi; Yamada, Takashi; Shoji, Takashi; Kawahara, Naoki

    2015-11-01

    X-ray fluorescence (XRF) analysis is a well-established analytical technique with a long research history. Many applications have been reported in various fields, such as in the environmental, archeological, biological, and forensic sciences as well as in industry. This is because XRF has a unique advantage of being a nondestructive analytical tool with good precision for quantitative analysis. Recent advances in XRF analysis have been realized by the development of new x-ray optics and x-ray detectors. Advanced x-ray focusing optics enables the making of a micro x-ray beam, leading to micro-XRF analysis and XRF imaging. A confocal micro-XRF technique has been applied for the visualization of elemental distributions inside the samples. This technique was applied for liquid samples and for monitoring chemical reactions such as the metal corrosion of steel samples in the NaCl solutions. In addition, a principal component analysis was applied for reducing the background intensity in XRF spectra obtained during XRF mapping, leading to improved spatial resolution of confocal micro-XRF images. In parallel, the authors have proposed a wavelength dispersive XRF (WD-XRF) imaging spectrometer for a fast elemental imaging. A new two dimensional x-ray detector, the Pilatus detector was applied for WD-XRF imaging. Fast XRF imaging in 1 s or even less was demonstrated for Euro coins and industrial samples. In this review paper, these recent advances in laboratory-based XRF imaging, especially in a laboratory setting, will be introduced.

  14. Solution-Phase Synthesis of Dipeptides: A Capstone Project That Employs Key Techniques in an Organic Laboratory Course

    ERIC Educational Resources Information Center

    Marchetti, Louis; DeBoef, Brenton

    2015-01-01

    A contemporary approach to the synthesis and purification of several UV-active dipeptides has been developed for the second-year organic laboratory. This experiment exposes students to the important technique of solution-phase peptide synthesis and allows an instructor to highlight the parallel between what they are accomplishing in the laboratory

  15. Effect of Parasitoid: Host Ratio and Group Size on Fitness of Spathius galinae (Hymenoptera: Braconidae): Implications for Mass-Rearing.

    PubMed

    Watt, Timothy J; Duan, Jian J; Tallamy, Douglas W; Hough-Goldstein, Judith

    2015-06-01

    Producing insect natural enemies in laboratories or insectaries for biological pest control is often expensive, and developing cost-effective rearing techniques is a goal of many biological control programs. Spathius galinae Belokobylskij and Strazenac (Hymenoptera: Braconidae), a newly described ectoparasitoid of emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is currently being evaluated for environmental introduction in the United States to provide biological control of this invasive pest. To improve mass-rearing outcomes for S. galinae, we investigated the effects of parasitoid: host ratio and parasitoid and host group size (density) on parasitoid fitness. Our results showed that when 1 emerald ash borer larva was exposed to 1, 2, 4, or 8 female parasitoids, parasitism rate was positively associated with increasing parasitoid: host ratio, while brood size, sex ratio, and fitness estimates of progeny were not affected. When a constant 1:1 parasitoid: host ratio was used, but group size varied from 1 female parasitoid and 1 host, 5 parasitoids and 5 hosts, 10 of each, and 20 of each in same size rearing cages, parasitism rates were highest when at least 5 females were exposed to 5 host larvae. Moreover, the number of progeny produced per female parasitoid was greatest when group size was 10 parasitoids and 10 hosts. These findings demonstrate that S. galinae may be reared most efficiently in moderately high-density groups (10 parasitoids and hosts) and with a 1:1 parasitoid: host ratio. PMID:26470215

  16. Sexual behavior and male volatile compounds in wild and mass-reared strains of the Mexican fruit fly Anastrepha ludens (Diptera: Tephritidae) held under different colony management regimes.

    PubMed

    Bosa, Carlos Felipe; Cruz-López, Leopoldo; Zepeda-Cisneros, Cristina Silvia; Valle-Mora, Javier; Guillén-Navarro, Karina; Liedo, Pablo

    2016-02-01

    We compared the calling and mating behavior and volatile release of wild males Anastrepha ludens (Loew) with males from 4 mass-reared strains: (i) a standard mass-reared colony (control), (ii) a genetic sexing strain (Tap-7), (iii) a colony started from males selected on their survival and mating competitiveness abilities (selected), and (iv) a hybrid colony started by crossing wild males with control females. Selected and wild males were more competitive, achieving more matings under field cage conditions. Mass-reared strains showed higher percentages of pheromone calling males under field conditions except for Tap-7 males, which showed the highest percentages of pheromone calling males under laboratory cage conditions. For mature males of all strains, field-cage calling behavior increased during the last hour before sunset, with almost a 2 fold increase exhibited by wild males during the last half hour. The highest peak mating activity of the 4 mass-reared strains occurred 30 min earlier than for wild males. By means of solid phase microextraction (SPME) plus gas chromatography-mass spectrometry (GC-MS), the composition of volatiles released by males was analyzed and quantified. Wild males emitted significantly less amounts of (E,E)-α-farnesene but emitted significantly more amounts of (E,E)-suspensolide as they aged than mass-reared males. Within the 4 mass-reared strains, Tap-7 released significantly more amounts of (E,E)-α-farnesene and hybrid more of (E,E)-suspensolide. Differences in chemical composition could be explained by the intrinsic characteristics of the strains and the colony management regimes. Characterization of calling behavior and age changes of volatile composition between wild and mass-reared strains could explain the differences in mating competitiveness and may be useful for optimizing the sterile insect technique in A. ludens. PMID:25339372

  17. Laboratory demonstration model: Active cleaning technique device. [for removal of contaminants from an optical surface

    NASA Technical Reports Server (NTRS)

    Shannon, R. L.; Gillette, R. B.

    1974-01-01

    The technique which utilizes exposure to a plasma to remove contaminants from a surface was incorporated into a laboratory model which demonstrates active cleaning by both plasma cleaning and ion sputtering modes of operation. The development phase is reported and includes discussion of the plasma tube configuration, device design, and performance tests. A general description of the active cleaning device is provided which includes information on the main power/plasma discharge sensors, and the power, gas supply, and ion accelerator systems. Development of the active cleaning species at high vacuum conditions is described and results indicate that plasma cleaning occurs in the region of a visible plume which extends from the end of the plasma tube. Recommendations are made for research to determine the plasma cleaning mechanism and the plasma species responsible for the cleaning, as well limitations on the type of contaminants that can be removed.

  18. Laboratory investigation of the erosion of cohesive sediments under oscillatory flows using a synchronized imaging technique

    NASA Astrophysics Data System (ADS)

    Sou, In Mei; Calantoni, Joseph; Reed, Allen; Furukawa, Yoko

    2012-11-01

    A synchronized dual stereo particle image velocimetry (PIV) measurement technique is used to examine the erosion process of a cohesive sediment core in the Small Oscillatory Flow Tunnel (S-OFT) in the Sediment Dynamics Laboratory at the Naval Research Laboratory, Stennis Space Center, MS. The dual stereo PIV windows were positioned on either side of a sediment core inserted along the centerline of the S-OFT allowing for a total measurement window of about 20 cm long by 10 cm high with sub-millimeter spacing on resolved velocity vectors. The period of oscillation ranged from 2.86 to 6.12 seconds with constant semi-excursion amplitude in the test section of 9 cm. During the erosion process, Kelvin-Helmholtz instabilities were observed as the flow accelerated in each direction and eventually were broken down when the flow reversed. The relative concentration of suspended sediments under different flow conditions was estimated using the intensity of light scattered from the sediment particles in suspension. By subtracting the initial light scattered from the core, the residual light intensity was assumed to be scattered from suspended sediments eroded from the core. Results from two different sediment core samples of mud and sand mixtures will be presented.

  19. Application of the one-minute preceptor technique by novice teachers in the gross anatomy laboratory.

    PubMed

    Chan, Lap Ki; Yang, Jian; Irby, David M

    2015-01-01

    The one-minute preceptor (OMP) was originally developed in the ambulatory care setting as a time-efficient teaching technique for learner-centered clinical training. There are also possible advantages of using the OMP in the gross anatomy laboratory. However, in a previous study it was found that providing training to experienced gross anatomy teachers in the use of the OMP did not result in improvement in students' perceptions of their learning, probably because of the fact that the experienced teachers had already developed their own pedagogical approaches. In the current study, we examined the effects of training novice teachers with about four years of gross anatomy teaching experience, in the use of the OMP in the gross anatomy laboratory, by surveying students to collect their views on their learning experiences, by observing the teachers' teaching behaviors before and after they were trained in OMP, and then by interviewing them. More students reported a better learning experience in the session after the teachers had been trained in the OMP than reported worse, in eight out of the nine items related to their learning experiences. The novice teachers were receptive to the OMP. After the OMP training, the novice teachers were observed to engage more in getting commitments from the students and in reinforcing what the students have done right, two of the five OMP microskills. They considered the OMP to be very useful for their development as anatomy teachers. PMID:25573139

  20. Soil examination for a forensic trace evidence laboratory--Part 1: Spectroscopic techniques.

    PubMed

    Woods, Brenda; Lennard, Chris; Kirkbride, K Paul; Robertson, James

    2014-12-01

    In the past, forensic soil examination was a routine aspect of trace evidence examination in forensic science. However, in Australia, the apparent need for soil examinations has diminished and with it the capability of forensic science laboratories to carry out soil examination has been eroded. In recent years, due to soil examinations contributing to some high profile investigations, interest in soil examinations has been renewed. Routine soil examinations conducted in a forensic science laboratory by trace evidence scientists can be facilitated if the examinations are conducted using the instrumentation routinely used by these examiners. Spectroscopic techniques such as visible microspectrophotometry (MSP) and Attenuated Total Reflectance (ATR) Fourier Transform Infrared spectroscopy (FTIR) are routinely used by trace evidence analysts for the colour and compositional analysis, respectively, of forensic items, including paints, fibres, inks and toners, tapes, adhesives and other miscellaneous examinations. This article presents an examination of the feasibility of using MSP and ATR-FTIR as a first step in the forensic comparison of soils with particular reference to Australian soil samples. This initial study demonstrates MSP and ATR-FTIR can effectively be used as a screening test for the discrimination of "forensic-sized" soil samples prior to submission for more detailed analyses by a soil expert. PMID:25205526

  1. 2. SOUTHEAST SIDE AND NORTHEAST REAR. SHOP BUILDING IN DISTANCE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. SOUTHEAST SIDE AND NORTHEAST REAR. SHOP BUILDING IN DISTANCE. NOTE CONCRETE PROTECTION SLAB FOR UNDERGROUND CONTROL ROOM AND ESCAPE HATCH ON GROUND AT RIGHT MIDDLE DISTANCE. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Firing Control Building, Test Area 1-100, northeast end of Test Area 1-100 Road, Boron, Kern County, CA

  2. 11. OBSERVATION POST NO. 3, NORTH SIDE AND WEST REAR, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. OBSERVATION POST NO. 3, NORTH SIDE AND WEST REAR, TEST STAND AT RIGHT. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Observation Bunkers for Test Stand 1-A, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA

  3. 7. SOUTH REAR. Looking northwest from corner of the Instrumentation ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. SOUTH REAR. Looking northwest from corner of the Instrumentation and Control Building (Building 8762). - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Test Stand 1-A, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA

  4. 5. BUILDING 8768, SOUTH SIDE AND EAST REAR. TEST STAND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. BUILDING 8768, SOUTH SIDE AND EAST REAR. TEST STAND 1A AT LEFT. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Observation Bunkers for Test Stand 1-A, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA

  5. 9. BUILDING 8769, EAST REAR AND NORTH SIDE, TEST STAND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. BUILDING 8769, EAST REAR AND NORTH SIDE, TEST STAND AT RIGHT. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Observation Bunkers for Test Stand 1-A, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA

  6. 3. BUILDING 8767, NORTH REAR AND WEST SIDE, TEST STAND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. BUILDING 8767, NORTH REAR AND WEST SIDE, TEST STAND 1-A AT FAR RIGHT. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Observation Bunkers for Test Stand 1-A, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA

  7. 3. BUILDING 8814, WEST SIDE AND SOUTH REAR, SHOWING BLAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. BUILDING 8814, WEST SIDE AND SOUTH REAR, SHOWING BLAST DOOR. BUILDING 8826 IS IN BACKGROUND. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Observation Bunker 1-D-3, Test Area 1-125, northwest end of Altair Boulevard, Boron, Kern County, CA

  8. 5. View northeast of rear of filtration bed building. Note ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. View northeast of rear of filtration bed building. Note monitor roof with clerestory windows over central corridor between filtration beds at center right of photograph. Laboratory building is at extreme center right of photograph. - Lake Whitney Water Filtration Plant, Filtration Plant, South side of Armory Street between Edgehill Road & Whitney Avenue, Hamden, New Haven County, CT

  9. 6. Detail view northeast of rear of filtration bed building. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Detail view northeast of rear of filtration bed building. Note monitor roof with clerestory windows over central corridor between filtration beds at center right of photograph. Laboratory building is at center right of photograph. - Lake Whitney Water Filtration Plant, Filtration Plant, South side of Armory Street between Edgehill Road & Whitney Avenue, Hamden, New Haven County, CT

  10. An Artificial Diet for Rearing Cochliomyia macellaria (Diptera: Calliphoridae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Larvae of the secondary screwworm, Cochliomyia macellaria (Fabricius), feed on carrion and may sometimes cause animal myiasis. They have been reared in the laboratory on various animal tissues to study their growth and development because of their importance in forensic science. We use the secondary...

  11. 3. SOUTHWEST REAR, WITH RAILROAD LINE AT RIGHT. HIGH PRESSURE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. SOUTHWEST REAR, WITH RAILROAD LINE AT RIGHT. HIGH PRESSURE HELIUM STORAGE TANKS AT LEFT. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Helium Compression Plant, Test Area 1-115, intersection of Altair & Saturn Boulevards, Boron, Kern County, CA

  12. 3. NORTHEAST REAR, SHOWING CONCRETE ENCASEMENT FOR STAIRWAY LEADING FROM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. NORTHEAST REAR, SHOWING CONCRETE ENCASEMENT FOR STAIRWAY LEADING FROM INSTRUMENT ROOM TO UNDERGROUND FIRING CONTROL ROOM. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Firing Control Building, Test Area 1-100, northeast end of Test Area 1-100 Road, Boron, Kern County, CA

  13. Laboratory insights into the detection of surface biosignatures by remote-sensing techniques

    NASA Astrophysics Data System (ADS)

    Poch, O.; Pommerol, A.; Jost, B.; Roditi, I.; Frey, J.; Thomas, N.

    2014-03-01

    With the progress of direct imaging techniques, it will be possible in the short or long-term future to retrieve more efficiently the information on the physical properties of the light reflected by rocky exoplanets (Traub et al., 2010). The search for visible-infrared absorption bands of peculiar gases (O2, CH4 etc.) in this light could give clues for the presence of life (Kaltenegger and Selsis, 2007). Even more uplifting would be the direct detection of life itself, on the surface of an exoplanet. Considering this latter possibility, what is the potential of optical remote-sensing methods to detect surface biosignatures? Reflected light from the surface of the Earth exhibits a strong surface biosignature in the form of an abrupt change of reflectance between the visible and infrared range of the spectrum (Seager et al., 2005). This spectral feature called "vegetation red-edge" is possibly the consequence of biological evolution selecting the right chemical structures enabling the plants to absorb the visible energy, while preventing them from overheating by reflecting more efficiently the infrared. Such red-edge is also found in primitive photosynthetic bacteria, cyanobacteria, that colonized the surface of the Earth ocean and continents billions of years before multicellular plants (Knacke, 2003). If life ever arose on an Earth-like exoplanet, one could hypothesize that some form of its surface-life evolves into similar photo-active organisms, also exhibiting a red-edge. In this paper, we will present our plan and preliminary results of a laboratory study aiming at precising the potentiality of remote sensing techniques in detecting such surface biosignatures. Using equipment that has been developed in our team for surface photometry studies (Pommerol 2011, Jost 2013, Pommerol 2013), we will investigate the reflectance spectra and bidirectional reflectance function of soils containing bacteria such as cyanobacteria, in various environmental conditions. We will also present our plan to incorporate polarization measurements, and particularly circular polarization, because it can be a marker of homochirality, which is supposed to be a universal property of life. Finally, the analyses of both biotic and abiotic materials will help to assess if (or in which peculiar conditions) remote sensing techniques can discriminate between false positive and strong biomarkers. Ultimately, these laboratory data can serve as reference data to guide and interpret future observations, paving the way for the detection of life on distant exoplanets.

  14. Laboratory study of the cross-hole resistivity tomography: The Model Stacking (MOST) Technique

    NASA Astrophysics Data System (ADS)

    Leontarakis, Konstantinos; Apostolopoulos, George V.

    2012-05-01

    Model experiments in the laboratory are used to find the optimal measuring and interpretation parameters that affect the quality of the results for the improved application of resistivity tomography between boreholes or between the surface and a tunnel. The experiments have shown that the detection ability of each crosshole electrode array is different and depends on the form of the sensitivity pattern. The bipole-bipole array, with current and potential pairs in different boreholes, has quite low signal and very good resolution of confined bodies in homogeneous half-spaces, but the resolution decreases as the half-space becomes more complex. The bipole-bipole array with the electrodes of each bipole in different boreholes produces a stronger signal, causing the models to be greatly influenced, even by the presence of small targets. However, the resolution quality is poor, mainly in the middle of the area between the boreholes. Pole-bipole array shows good resolution of the targets detection, but it is less accurate when conditions become noisier. The pole-tripole array has the best resolution, even when the environment becomes more complex or noisier, but the produced models have also many artifacts. The combined arrays' data inversion yields the greatest influence of the targets on resistivity models, usually with very good positioning or shape resolution, but with many more intense artifacts, since this method inevitably combines the advantages and disadvantages of each array. A new approach to improve the quality of resistivity models has been developed, based on a stacking technique, through the processing of different arrays' inverted models ("MOST" technique). The improvement of the MOST models' quality has been measured, comparing each final resistivity model with the corresponding real one, highlighting this way the efficiency of this technique, in contrary to the combined arrays' data inversion.

  15. Post-release survival of hand-reared and parent-reared Mississippi sandhill cranes

    USGS Publications Warehouse

    Ellis, D.H.; Gee, G.F.; Hereford, Scott G.; Olsen, G.H.; Chisolm, T.D.; Nicolich, J.M.; Sullivan, K.A.; Thomas, N.J.; Nagendran, M.; Hatfield, J.S.

    2000-01-01

    The Mississippi Sandhill Crane (Grus canadensis pulla) reintroduction program is the largest crane reintroduction effort in the world. Here we report on a 4-year experiment in which we compared post-release survival rates of 56 hand-reared and 76 parent-reared Mississippi Sandhill Cranes. First-year survival was 80%. Surprisingly, hand-reared cranes survived better than parent-reared birds, and the highest survival rates were for hand-reared juveniles released in mixed cohorts with parent-reared birds. Mixing improved survival most for parent-reared birds released with hand-reared birds. These results demonstrate that hand-rearing can produce birds which survive at least as well as parent-reared birds and that improved survival results from mixing hand-reared and parent-reared birds.

  16. Post-release survival of hand-reared and parent-reared Mississippi Sandhill Cranes

    USGS Publications Warehouse

    Ellis, D.H.; Gee, G.F.; Hereford, Scott G.; Olsen, G.H.; Chisolm, T.D.; Nicolich, J.M.; Sullivan, K.A.; Thomas, N.J.; Nagendran, M.; Hatfield, J.S.

    2000-01-01

    The Mississippi Sandhill Crane (Grus canadensis pulla) reintroduction program is the largest crane reintroduction effort in the world. Here we report on a 4-year experiment in which we compared post-release survival rates of 56 hand-reared and 76 parent-reared Mississippi Sandhill Cranes. First-year survival was 80%. Surprisingly, hand-reared cranes survived better than parent-reared birds, and the highest survival rates were for hand-reared juveniles released in mixed cohorts with parent-reared birds. Mixing improved survival most for parent-reared birds released with hand-reared birds. These results demonstrate that hand-rearing can produce birds which survive at least as well as parent-reared birds and that improved survival results from mixing hand-reared and parent-reared birds.

  17. A laboratory comparison of chemical volcanic gas sampling techniques using an artificial fumarole

    NASA Astrophysics Data System (ADS)

    Mioduszewski, L.; Kress, V.

    2006-12-01

    Volcanic gases play a critical role in nearly all volcanic processes. A number of chemical and spectroscopic techniques have been developed in recent decades to measure volcanic gas output. Giggenbach bottles, alkaline traps, filter packs, and venturi samplers are examples of chemical gas sampling techniques involving the reaction of acidic gas with an alkaline solution or a solid hydroxide salt on a filter. Aside from Giggenbach bottles all of these methods are disequilibrium techniques relying on the assumption that the kinetics of gas reaction are identical for all species. Given that equilibrium species solubilities differ, there is reason to suspect this assumption. While these chemical sampling methods have been used extensively in the field, we are aware of no direct calibration in a lab setting. To address this problem we have built an artificial fumarole in the laboratory for quantitative comparison and evaluation of gas sampling techniques. The fumarole consists of a metered mixture of H2 CO2 SO2, CO gas combined in a high-temperature mixing chamber with an aqueous solution of HCl and HF. The resulting equilibrium high-temperature gas is sampled at the furnace exit. Several gas mixtures were considered representing a range of compositions encountered at natural fumaroles. Results of analysis are compared with equilibrium gas concentrations calculated from the input gas composition and furnace temperature. We compared Giggenbach bottles, a variety of filter-pack chemistries, and passive alkaline trap samplers. In addition, we tested a venturi sampler of our own design; optimized for ruggedness, ease of field use, reliability and sampling efficiency. Each sample was analyzed by ion chromatography both before and after oxidation with H2O2 for fluoride, chloride, and several sulfur species. While reduced sulfur is not easily analyzed by IC, it can be determined from the difference in concentrations between total S in the oxidized samples and the other sulfur species in the non-oxidized samples. Preliminary results show that the alkaline trap is the least accurate method, while the remaining techniques show varying degrees of success. Our redesigned venturi sampler works particularly well and promises to be a very successful field instrument.

  18. A laboratory course for teaching laboratory techniques, experimental design, statistical analysis, and peer review process to undergraduate science students.

    PubMed

    Gliddon, C M; J Rosengren, R

    2012-01-01

    This article describes a 13-week laboratory course called Human Toxicology taught at the University of Otago, New Zealand. This course used a guided inquiry based laboratory coupled with formative assessment and collaborative learning to develop in undergraduate students the skills of problem solving/critical thinking, data interpretation and written discussion of results. The laboratory practices were a guided inquiry based around retinol's ability to potentiate acetaminophen-mediated hepatotoxicity. To induce critical thinking, students were given a choice as to which assay they could use to determine how retinol affected acetaminophen hepatotoxicity. Short summaries were handed in following each assay and formed the bases of the formative assessment. To complete the feedback loop, a summative assessment that consisted of all the graphs and concepts from the short summaries were combined into a manuscript. To give the students exposure to science communication, the manuscript had to be written in accordance to the submission guidelines for Toxicological Sciences. Evaluation of this course was determined by a student questionnaire using a Likert scale and students' responses were very favorable. While the subject matter was toxicological centric, the content could be easily modified to suit another subject matter in biochemistry and molecular biology. PMID:23166024

  19. Visualization of DNA in agarose gels as migrating colored bands: Applications to laboratory techniques

    SciTech Connect

    Adkins, S.; Burmeister, M.

    1994-09-01

    We have developed a method to visualize DNA without the use of ethidium bromide and UV radiation. Anionic dyes (colored anion) have long been used in the detection of pharmaceutical amines via ion pairing, here we show that cationic dyes may be used to detect DNA. In gel electrophoresis in which DNA is traveling toward the positive electrode and a cationic dye is traveling toward the negative electrode, we expect ion pairing of the DNA and the dye as they meet in the gel. The dye should bind to the anionic DNA. If the DNA is not completely neutralized by the dye, it should continue to migrate. Ethidium bromide, which is believed to stain DNA primarily by intercalation between bases, exhibits the fluorescence through its cation and also may bind to DNA, to some extent, through ionic pairing. We observed that DNA forms colored bands during electrophoresis in standard agarose gels when a cationic dye is present in the gel and running buffer. DNA in amounts equal to or greater than 80 ng is seen as a discrete migrating colored band in ambient room lighting. Colored bands may be transferred to nitrocellulose by vacuum transfer in room temperature gel dryer, Xeroxed, fixed with NaOH and dye removed with dilute detergent. Also, isolation of DNA bands from preparative gels may be accomplished without the typical use of ethidium bromide and UV radiation which are known to alter DNA and pose hazards to laboratory personnel. We are presently investigating the general utility of using dyes to visualize DNA for various laboratory techniques.

  20. Integrating Novel Field, Laboratory and Modelling Techniques to Upscale Estimates of Soil Erosion

    NASA Astrophysics Data System (ADS)

    Wainwright, John; Parsons, Anthony; Cooper, James; Long, Edward; Hargrave, Graham; Kitchener, Ben; Hewett, Caspar; Onda, Yuichi; Furukawa, Tomomi; Obana, Eiichiro; Hayashi, Hirofumi; Noguchi, Takehiro

    2013-04-01

    Erosion is a particle-based phenomenon, yet most of current understanding and modelling of this process is based on bulk measurements rather than the movement of individual particles. Difficulties with measuring particle motions in dynamically changing conditions are being overcome with the application of two new technologies - particle imaging velocimetry (PIV) and radio frequency identification (RFID). It is thus possible to evaluate the entrainment, transport and deposition of individual particles and these data can be used to parameterize and to test particle-based modelling of the particle-based process. Both PIV and RFID tagging have been used in laboratory experiments to evaluate the detachment process by raindrops on bare surfaces and in shallow flows using rainfall simulation. The results suggest that the processes are more complex than hitherto thought with multiple detachment and transfer mechanisms. Because both mechanisms affect travel distance, they affect the ways in which estimates of soil erosion can be scaled from plot to hillslope and catchment scales. To evaluate movements at larger scales, we have also used RFID-tagged particles in field settings to look at sediment transfers following the Fukushima accident in Japan, 2011. A marker-in-cell model (MAHLERAN-MiC) has been developed to enable the laboratory results to be upscaled and tested in a field setting. Markers (representing sediment particles), containing sediment-property information, are initially distributed on a cellular grid. A cellular model is used to set up the boundary conditions and determine the hydrology and hydraulics on the hillslope. The markers are then moved through the grid according to these properties. This technique combines the advantages of Eulerian and Lagrangian methods while avoiding the shortcomings of each (computational efficiency vs. accuracy). The model simulates all the processes of detachment and transport; raindrop detachment and transport, interrill erosion, concentrated erosion (bedload transport) and suspended sediment transport. We demonstrate how distributions of travel distances change as spatial and temporal scales of application change, and thereby how the laboratory and field experiments can be best used to develop more robust approaches to the upscaling of estimates of erosion rates.

  1. Current updates on laboratory techniques for the diagnosis of male reproductive failure

    PubMed Central

    Sikka, Suresh C; Hellstrom, Wayne JG

    2016-01-01

    The incidence of male reproductive failure leading to infertility, whether due to delayed parenthood, environmental issues, genetic factors, drugs, etc., is increasing throughout the world. The diagnosis and prognosis of male subfertility have become a challenge. While the basic semen assessment has been performed for many years, a number of studies question the value of the traditional semen characteristics. This is partly due to inadequate methods and standardization, limited knowledge of technical requirements for quality assurance, and an incomplete understanding of what clinical information a semen assessment can provide. Laboratories currently performing semen and endocrine assessment show great variability. The World Health Organization (WHO) manual for the evaluation of semen has been the core of andrology and fertility evaluation that has helped in further development of this field over many years. These include the physical appearance of the ejaculate, assessments of sperm count, motility, vitality, morphology, and functional aspects of the sperm and semen sample. These tests also include male endocrine profile, biochemical evaluation of the semen, detection of antisperm antibodies in serum, the use of computer-aided sperm analysis (CASA), sperm DNA integrity, and its damage due to oxidative stress. Assisted reproductive techniques (e.g., IVF, ICSI) have shown great success but are too expensive. Further development in this field with newer techniques and extensive training/instructions can improve accuracy and reduce variability, thus maintaining the quality and standards of such an evaluation. There is an urgent need to have standardized training centers and increased awareness in this area of men's health for reproductive success. PMID:27056346

  2. Current updates on laboratory techniques for the diagnosis of male reproductive failure.

    PubMed

    Sikka, Suresh C; Hellstrom, Wayne Jg

    2016-01-01

    The incidence of male reproductive failure leading to infertility, whether due to delayed parenthood, environmental issues, genetic factors, drugs, etc., is increasing throughout the world. The diagnosis and prognosis of male subfertility have become a challenge. While the basic semen assessment has been performed for many years, a number of studies question the value of the traditional semen characteristics. This is partly due to inadequate methods and standardization, limited knowledge of technical requirements for quality assurance, and an incomplete understanding of what clinical information a semen assessment can provide. Laboratories currently performing semen and endocrine assessment show great variability. The World Health Organization (WHO) manual for the evaluation of semen has been the core of andrology and fertility evaluation that has helped in further development of this field over many years. These include the physical appearance of the ejaculate, assessments of sperm count, motility, vitality, morphology, and functional aspects of the sperm and semen sample. These tests also include male endocrine profile, biochemical evaluation of the semen, detection of antisperm antibodies in serum, the use of computer-aided sperm analysis (CASA), sperm DNA integrity, and its damage due to oxidative stress. Assisted reproductive techniques (e.g., IVF, ICSI) have shown great success but are too expensive. Further development in this field with newer techniques and extensive training/instructions can improve accuracy and reduce variability, thus maintaining the quality and standards of such an evaluation. There is an urgent need to have standardized training centers and increased awareness in this area of men's health for reproductive success. PMID:27056346

  3. Nonculture molecular techniques for diagnosis of bacterial disease in animals: a diagnostic laboratory perspective.

    PubMed

    Cai, H Y; Caswell, J L; Prescott, J F

    2014-03-01

    The past decade has seen remarkable technical advances in infectious disease diagnosis, and the pace of innovation is likely to continue. Many of these techniques are well suited to pathogen identification directly from pathologic or clinical samples, which is the focus of this review. Polymerase chain reaction (PCR) and gene sequencing are now routinely performed on frozen or fixed tissues for diagnosis of bacterial infections of animals. These assays are most useful for pathogens that are difficult to culture or identify phenotypically, when propagation poses a biosafety hazard, or when suitable fresh tissue is not available. Multiplex PCR assays, DNA microarrays, in situ hybridization, massive parallel DNA sequencing, microbiome profiling, molecular typing of pathogens, identification of antimicrobial resistance genes, and mass spectrometry are additional emerging technologies for the diagnosis of bacterial infections from pathologic and clinical samples in animals. These technical advances come, however, with 2 caveats. First, in the age of molecular diagnosis, quality control has become more important than ever to identify and control for the presence of inhibitors, cross-contamination, inadequate templates from diagnostic specimens, and other causes of erroneous microbial identifications. Second, the attraction of these technologic advances can obscure the reality that medical diagnoses cannot be made on the basis of molecular testing alone but instead through integrated consideration of clinical, pathologic, and laboratory findings. Proper validation of the method is required. It is critical that veterinary diagnosticians understand not only the value but also the limitations of these technical advances for routine diagnosis of infectious disease. PMID:24569613

  4. Blood supply to the thoracolumbar spinal cord in the laboratory mouse using corrosion and dissection techniques.

    PubMed

    Flesarova, Slavka; Mazensky, David; Teleky, Jana; Almasiova, Viera; Holovska, Katarina; Supuka, Peter

    2016-01-01

    Mice are used frequently as experimental models in the study of ischemic spinal cord injury. The aim of the present study was to describe the arterial blood supply to the thoracolumbar spinal cord in the mouse. The study was carried out on 20 adult mice using the corrosion and dissection technique. Dorsal intercostal arteries were found as branches of the thoracic aorta: as 7 pairs in 80% of cases, as 8 pairs in 15% of cases and as 9 pairs in 5% of cases. The paired lumbar arteries arising from the abdominal aorta were present as 5 pairs in all cases. Along the entire thoracic and lumbar spinal regions, we observed left-sided branches entering the ventral spinal artery in 64.2% and right-sided branches in 35.8% of cases. Along the entire thoracic and lumbar spinal regions, the branches entering the dorsal spinal arteries were left-sided in 60.8% of cases and right-sided in 39.2% of cases. We found some variations in the site of origin of the artery of Adamkiewicz and in the number of dorsal spinal arteries. Documenting the anatomical variations in spinal cord blood supply in the laboratory mouse will aid the planning of future experimental studies and in determining the clinical relevance of such studies. PMID:25636913

  5. Establishment of a primary reference solar cell calibration technique in Korea: methods, results and comparison with WPVS qualified laboratories

    NASA Astrophysics Data System (ADS)

    Ahn, SeungKyu; Ahn, SeJin; Yun, Jae Ho; Lee, Dong-Hoon; Winter, Stefan; Igari, Sanekazu; Yoon, KyungHoon

    2014-06-01

    A primary reference solar cell calibration technique recently established at the Korea Institute of Energy Research in Korea is introduced. This calibration technique is an indoor method that uses a highly collimated continuous-type solar simulator and absolute cavity radiometer traceable to the World Radiometric Reference. The results obtained using this calibration technique are shown with a precise uncertainty analysis, and the system configuration and calibration procedures are introduced. The calibration technique avoids overestimating the short-circuit current of a reference solar cell due to multiple reflections of incident simulator light using a novel method. In addition, the uncertainty analysis indicates that the calibration technique has an expanded uncertainty of approximately 0.7% with a coverage factor of k = 2 for a c-Si reference cell calibration. In addition, the developed primary reference solar cell calibration technique was compared with other techniques established in the World Photovoltaic Scale (WPVS) qualified calibration laboratories to verify its validity and reliability.

  6. Laboratory investigation of the erosion of cohesive sediments under oscillatory flows using a synchronized imaging technique

    NASA Astrophysics Data System (ADS)

    Sou, I.; Calantoni, J.; Reed, A. H.; Furukawa, Y.

    2012-12-01

    A synchronized dual stereo particle image velocimetry (PIV) measurement technique is used to examine the erosion process of a cohesive sediment core in the Small Oscillatory Flow Tunnel (S-OFT) in the Sediment Dynamics Laboratory at the Naval Research Laboratory, Stennis Space Center, MS. The PIV system uses four cameras and a dual cavity Nd:YAG laser. The system allows for a pair of stereo PIV windows of about 10 cm by 10 cm each to be arbitrarily located within a single light sheet. Image pairs were acquired with all four cameras at 50 Hz for 50 consecutive seconds for each flow condition. The stereo PIV windows were positioned on either side of sediment cores inserted along the centerline of the S-OFT allowing for a total measurement window of about 20 cm long by 10 cm high with sub-millimeter spacing on resolved velocity vectors. The oscillatory flows are generated by two types of driving mechanism (scotch yoke and crank lever) for converting the rotational motion of the flywheel into the linear motion of a piston. The period of oscillation ranged from 2.86 to 6.12 seconds with constant semi-excursion amplitude in the test section of 9 cm. Two kinds of inorganic sediment samples were examined. One was a mixture of 50% kaolinite and 50% 500-micron sand under flows driven by the crank lever mechanism. Another sediment core was a mixture of 50% mud collected in Galveston Bay, TX, and 50% 250-micron sand under flows driven by the scotch-yoke mechanism. During the erosion process, Kelvin-Helmholtz instabilities were observed as the flow accelerated in each direction and eventually were broken down when the flow reversed. An example of the instantaneous velocity field superimposed on the raw image is shown in Figure 1. The relative concentration of suspended sediments under different flow conditions was estimated using the intensity of light scattered from the sediment particles in suspension. By subtracting the initial light scattered from the mud core, the residual light intensity was assumed to be scattered from suspended sediments eroded from the core. Relative comparisons were only made using the same sample mixture since it is difficult, if not impossible, to calibrate the light scattering from different sediments.; Figure 1. An example of the instantaneous time-resolved velocity field superimposed on the raw image.

  7. Jamaican Child-Rearing Practices: The Role of Corporal Punishment.

    ERIC Educational Resources Information Center

    Smith, Delores E.; Mosby, Gail

    2003-01-01

    Examines child-rearing techniques of Jamaican adults and their assumed effects on child outcomes. Also examines the plausibility of the assumption that harsh physical punishment meted out to children is partially responsible for current social problems of that nation. Recommends approaches to tackle the broad goals of addressing familial and…

  8. Solution-Phase Synthesis of Dipeptides: A Capstone Project That Employs Key Techniques in an Organic Laboratory Course

    ERIC Educational Resources Information Center

    Marchetti, Louis; DeBoef, Brenton

    2015-01-01

    A contemporary approach to the synthesis and purification of several UV-active dipeptides has been developed for the second-year organic laboratory. This experiment exposes students to the important technique of solution-phase peptide synthesis and allows an instructor to highlight the parallel between what they are accomplishing in the laboratory…

  9. Physics Laboratory Investigation of Vocational High School Field Stone and Concrete Construction Techniques in the Central Java Province (Indonesia)

    ERIC Educational Resources Information Center

    Purwandari, Ristiana Dyah

    2015-01-01

    The investigation aims in this study were to uncover the observations of infrastructures and physics laboratory in vocational high school for Stone and Concrete Construction Techniques Expertise Field or Teknik Konstruksi Batu dan Beton (TKBB)'s in Purwokerto Central Java Province, mapping the Vocational High School or Sekolah Menengah Kejuruan…

  10. Effectiveness of Student-Generated Video as a Teaching Tool for an Instrumental Technique in the Organic Chemistry Laboratory

    ERIC Educational Resources Information Center

    Jordan, Jeremy T.; Box, Melinda C.; Eguren, Kristen E.; Parker, Thomas A.; Saraldi-Gallardo, Victoria M.; Wolfe, Michael I.; Gallardo-Williams, Maria T.

    2016-01-01

    Multimedia instruction has been shown to serve as an effective learning aid for chemistry students. In this study, the viability of student-generated video instruction for organic chemistry laboratory techniques and procedure was examined and its effectiveness compared to instruction provided by a teaching assistant (TA) was evaluated. After…

  11. Effectiveness of Student-Generated Video as a Teaching Tool for an Instrumental Technique in the Organic Chemistry Laboratory

    ERIC Educational Resources Information Center

    Jordan, Jeremy T.; Box, Melinda C.; Eguren, Kristen E.; Parker, Thomas A.; Saraldi-Gallardo, Victoria M.; Wolfe, Michael I.; Gallardo-Williams, Maria T.

    2016-01-01

    Multimedia instruction has been shown to serve as an effective learning aid for chemistry students. In this study, the viability of student-generated video instruction for organic chemistry laboratory techniques and procedure was examined and its effectiveness compared to instruction provided by a teaching assistant (TA) was evaluated. After

  12. Teaching laboratory rodent research techniques under the tenets of situated learning improves student confidence and promotes collaboration.

    PubMed

    Whitcomb, Tiffany L; Taylor, Edward W

    2014-07-01

    A targeted needs assessment at our institution revealed that the online system used to train researchers on performing techniques with animals did not provide opportunities to practice skills, introduce learners to animal care staff, nor satisfactorily support researchers' needs to become comfortable with laboratory animal species. To correct these deficiencies, a series of hands-on training sessions, framed theoretically in situated learning, was developed. This theoretical framework asserts that learning for everyday living (in this case, performing laboratory animal techniques) happens when people interact within the community while using the 'tools at hand' (that is, the instruments and jargon of the field). From this perspective, the students work alongside the instructor as apprentices. The instructor creates increasingly challenging learning opportunities as students work toward independently performing techniques. To test our hypothesis that teaching from this perspective improves comfort levels with laboratory animals and promotes collaborative relationships between animal care and research personnel, a mixed-method design involving online surveys (first survey, n = 45; second survey, n = 35) and semistructured interviews (n = 10) was used. Quantitative results revealed that students became more comfortable with laboratory animals and were more likely to contact animal care personnel due to participating in the training program. The qualitative arm of the study identified specific features of the training program that improved comfort levels for students (seeing then doing, working in small groups, learning within a comfortable environment, and building collegial relationships). These results support teaching rodent research techniques from the practical and theoretical approach of situated learning. PMID:25199092

  13. Teaching Discrete and Programmable Logic Design Techniques Using a Single Laboratory Board

    ERIC Educational Resources Information Center

    Debiec, P.; Byczuk, M.

    2011-01-01

    Programmable logic devices (PLDs) are used at many universities in introductory digital logic laboratories, where kits containing a single high-capacity PLD replace "standard" sets containing breadboards, wires, and small- or medium-scale integration (SSI/MSI) chips. From the pedagogical point of view, two problems arise in these laboratories.…

  14. Teaching Discrete and Programmable Logic Design Techniques Using a Single Laboratory Board

    ERIC Educational Resources Information Center

    Debiec, P.; Byczuk, M.

    2011-01-01

    Programmable logic devices (PLDs) are used at many universities in introductory digital logic laboratories, where kits containing a single high-capacity PLD replace "standard" sets containing breadboards, wires, and small- or medium-scale integration (SSI/MSI) chips. From the pedagogical point of view, two problems arise in these laboratories.

  15. LABORATORY EVALUATION OF NO(SUB X) REDUCTION TECHNIQUES FOR REFINERY CO BOILERS

    EPA Science Inventory

    The report describes a laboratory test program to investigate NOx emissions from refinery CO boilers. The program had three major objectives: (1) to simulate in the laboratory a full-scale refinery CO boiler, (2) to investigate the effects of operational variables on NOx formatio...

  16. Laboratory instrumentation and techniques for characterizing multi-junction solar cells for space applications

    NASA Astrophysics Data System (ADS)

    Woodyard, James R.

    1995-10-01

    Multi-junction solar cells are attractive for space applications because they can be designed to convert a larger fraction of AMO into electrical power at a lower cost than single-junction cells. The performance of multi-junction cells is much more sensitive to the spectral irradiance of the illuminating source than single-junction cells. The design of high efficiency multi-junction cells for space applications requires matching the optoelectronic properties of the junctions to AMO spectral irradiance. Unlike single-junction cells, it is not possible to carry out quantum efficiency measurements using only a monochromatic probe beam and determining the cell short-circuit current assuming linearity of the quantum efficiency. Additionally, current-voltage characteristics can not be calculated from measurements under non-AMO light sources using spectral-correction methods. There are reports in the literature on characterizing the performance of multi junction cells by measuring and convoluting the quantum efficiency of each junction with the spectral irradiance; the technique is of limited value for the characterization of cell performance under AMO power-generating conditions. We report the results of research to develop instrumentation and techniques for characterizing multi junction solar cells for space . An integrated system is described which consists of a standard lamp, spectral radiometer, dual-source solar simulator, and personal computer based current-voltage and quantum efficiency equipment. The spectral radiometer is calibrated regularly using the tungsten-halogen standard lamp which has a calibration based on NIST scales. The solar simulator produces the light bias beam for current-voltage and cell quantum efficiency measurements. The calibrated spectral radiometer is used to 'fit' the spectral irradiance of the dual-source solar simulator to WRL AMO data. The quantum efficiency apparatus includes a monochromatic probe beam for measuring the absolute cell quantum efficiency at various voltage biases, including the voltage bias corresponding to the maximum-power point under AMO light bias. The details of the procedures to 'fit' the spectral irradiance to AMO will be discussed. An assessment of the role of the accuracy of the 'fit' of the spectral irradiance and probe beam intensity on measured cell characteristics will be presented. quantum efficiencies were measured with both spectral light bias and AMO light bias; the measurements show striking differences. Spectral irradiances were convoluted with cell quantum efficiencies to calculate cell currents as function of voltage. The calculated currents compare with measured currents at the 1% level. Measurements on a variety of multi-junction cells will be presented. The dependence of defects in junctions on cell quantum efficiencies measured under light and voltage bias conditions will be presented. techniques. &Expeditious development of multi-junction solar cell technology for space presents challenges for cell characterization in the laboratory.

  17. Clay identification and amount measured by laboratory techniques compared to well log responses: Application to tight gas sands and shales

    NASA Astrophysics Data System (ADS)

    Heinze, D. M.

    1985-03-01

    Identification and amount of clay in tight sands and shales calculated by downhole log interpretation techniques have been compared with those measured by a variety of analytical laboratory methods. The US DOE's Multiwell Experiment in the Piceance Creek Basin, Colorado has provided a wide selection of core and extensive logging data from the Mesaverde Formation. The samples were analyzed for clay content in the laboratory using all of the following methods: optical petrography, X-ray diffraction, bulk chemical, acetate method for cation exchange capacity, sedigraph, pipet, and centrifuge. Potassium, uranium, and thorium (KUT) concentrations were determined utilizing the following techniques: X-ray fluorescence, atomic absorption, neutron activation, gamma spectroscopy and alpha spectrometry. This multiple technique approach was used in order to corroborate laboratory data. The results clearly show that relying on one or two methods can result in significant error. Laboratory data point out that this small sampling of log-defined good sands and shales actually represents a variety of lithologies and compositions.

  18. Development of a Natural Rearing System to Improve Supplemental Fish Quality, 1999-2003 Progress Report.

    SciTech Connect

    Maynard, Desmond J.

    2003-02-25

    The National Marine Fisheries Service (NMFS) has been conducting Natural Rearing Enhancement System (NATURES) research since the early 1990s. NATURES studies have looked at a variety of mechanisms to enhance production of wild-like salmonids from hatcheries. The goal of NATURES research is to develop fish culture techniques that enable hatcheries to produce salmon with more wild-like characteristics and increased postrelease survival. The development of such techniques is called for in the Columbia Basin Fish and Wildlife Program. This document is the draft report for the Supplemental Fish Quality Contract DE-AI79-91BP20651 Over the history of the project, the effects of seminatural raceway habitats, automated underwater feeders, exercise current velocities, live food diets, and predator avoidance training have been investigated. The findings of these studies are reported in an earlier contract report (Maynard et al. 1996a). The current report focuses on research that has been conducted between 1999 and 2002. This includes studies on the effect of exercise on salmon and steelhead trout, effects of predator avoid training, integration of NATUES protocols into production hatcheries, and the study of social behavior of steelhead grown in enriched and conventional environments. Traditionally, salmon (Oncorhynchus spp.) are reared in barren concrete raceways that lack natural substrate, in-stream structure, or overhead cover. The fish are fed in an unnatural manner with artificial feeds mechanically or hand broadcast across the water surface. This traditional approach has increased the egg-to-smolt survival of hatchery-reared fish by an order of magnitude over that experienced by wild-reared salmon. However, once hatchery-reared fish are released into the wild their smolt-to-adult survival is usually much lower than wild-reared salmon. The reduced postrelease survival of hatchery-reared fish may stem from differences in their behavior and morphology compared to wild-reared salmon. After release, hatchery-reared fish are inefficient foragers and are often found with empty stomachs or stomachs filled with indigestible debris (Miller 1953, Hochachka 1961, Reimers 1963, Sosiak et al. 1979, Myers 1980, O'Grady 1983, Johnsen and Ugedal 1986). Their social behavior also differs, with hatchery-reared fish congregating at higher densities, being more aggressive, and displaying less territory fidelity than wild-reared fish (Fenderson et al. 1968, Bachman 1984, Swain and Riddell 1990). In the natural environment this results in hatchery-reared fish spending more time in high-risk aggressive behavior and less time in beneficial foraging behavior than their wild-reared counterparts. Hatchery-reared fish are also more surface oriented than wild-reared salmonids (Mason et al. 1967, Sosiak 1978). This increases their risk of being attacked by avian predators, such as kingfishers (Ceryle spp.), which search for fish near the surface. Although some of the differences between wild and hatchery-reared fish are innate (Reisenbichler and McIntyre 1977, Swain and Riddell 1990), many are conditioned and can be modified by altering the hatchery rearing environment. NATURES studies are aimed at developing a more natural salmon culture environment to prevent the development of these unnatural attributes in hatchery-reared fish. NATURES fish culture practices are already producing salmon with up to about 50% higher in-stream survival than conventionally-reared fish (Maynard et al. 1996b). When these techniques are incorporated into production releases, they should also translate into increased smolt-to-adult survival. Conservation and supplementation programs can use NATURES-reared salmonids to rebuild stocks currently listed as endangered and threatened into healthy self-sustaining runs more rapidly than traditional programs. Traditional production programs can also use high-survival NATURES-reared fish to reduce their impact on wild populations, while still meeting their adult mitigation goals.

  19. Pollution prevention in the analytical laboratory--Microscale and other techniques do add up

    SciTech Connect

    Erickson, M.D.; Alvarado, J.S.; Lu, C.-S.; Peterson, D.P.; Silzer, J.

    1996-07-01

    The principles of pollution prevention in the analytical laboratory have not been addressed sufficiently. Although the amount of reagent used per sample is often only a few milliliters, the aggregate of many routine test each day in thousands of laboratories becomes significant. Current recycling practices are not practical with small streams. Therefore, we have adopted the principles of microscale chemistry, along with other modern analytical approaches, to develop routine analytical methods that significantly curtail waste but still maintain acceptable analytical figures of merit and achieve cost savings through reduced reagent consumption and reduced labor cost.

  20. Teaching Laboratory Rodent Research Techniques under the Tenets of Situated Learning Improves Student Confidence and Promotes Collaboration

    PubMed Central

    Whitcomb, Tiffany L; Taylor, Edward W

    2014-01-01

    A targeted needs assessment at our institution revealed that the online system used to train researchers on performing techniques with animals did not provide opportunities to practice skills, introduce learners to animal care staff, nor satisfactorily support researchers’ needs to become comfortable with laboratory animal species. To correct these deficiencies, a series of hands-on training sessions, framed theoretically in situated learning, was developed. This theoretical framework asserts that learning for everyday living (in this case, performing laboratory animal techniques) happens when people interact within the community while using the ‘tools at hand’ (that is, the instruments and jargon of the field). From this perspective, the students work alongside the instructor as apprentices. The instructor creates increasingly challenging learning opportunities as students work toward independently performing techniques. To test our hypothesis that teaching from this perspective improves comfort levels with laboratory animals and promotes collaborative relationships between animal care and research personnel, a mixed-method design involving online surveys (first survey, n = 45; second survey, n = 35) and semistructured interviews (n = 10) was used. Quantitative results revealed that students became more comfortable with laboratory animals and were more likely to contact animal care personnel due to participating in the training program. The qualitative arm of the study identified specific features of the training program that improved comfort levels for students (seeing then doing, working in small groups, learning within a comfortable environment, and building collegial relationships). These results support teaching rodent research techniques from the practical and theoretical approach of situated learning. PMID:25199092

  1. "Audibilization" in the Chemistry Laboratory: An Introduction to Correlation Techniques for Data Extraction

    ERIC Educational Resources Information Center

    Hovick, James W.; Murphy, Michael; Poler, J. C.

    2007-01-01

    The study describes the development and advantages of various correlation techniques that are used for data extraction and are integral to all modern instrumentation. The "Audibilization" of the electronic signals in such cases is found to be very essential for the technique.

  2. Performance of marking techniques in the field and laboratory for Diabrotica speciosa (Germar) (Coleoptera: Chrysomelidae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A reliable marking technique was needed for a mark-release-recapture experiment with adults of Diabrotica speciosa (Germar). Four marking techniques, acrylic paint (spattered or brushed on the surface of the insect); and fluorescent pigments (dusted on surfaces or mixed with diet to produce an inges...

  3. Teaching Protein Purification and Characterization Techniques: A Student-Initiated, Project-Oriented Biochemistry Laboratory Course

    ERIC Educational Resources Information Center

    MacDonald, Gina

    2008-01-01

    This report describes a biochemistry laboratory that is completely project-oriented. Upper-level biology and chemistry majors work in teams to purify a protein of their choice. After the student groups have completed literature searches, ordered reagents, and made buffers they continue to learn basic protein purification and biochemical techniques…

  4. Computer Modeling of Microbiological Experiments in the Teaching Laboratory: Animation Techniques.

    ERIC Educational Resources Information Center

    Tritz, Gerald J.

    1987-01-01

    Discusses the use of computer assisted instruction in the medical education program of the Kirksville College of Osteopathic Medicine (Missouri). Describes the animation techniques used in a series of simulations for microbiology. (TW)

  5. Applying risk-based M&S VV&A techniques to test and laboratory facilities

    NASA Astrophysics Data System (ADS)

    Smith, Jeremy; Elele, James; Hall, David; Pedriani, Charles

    2011-06-01

    The Department of Defense (DOD) is exercising a risk-based process for verifying, validating and accrediting models and simulations (M&S) used in system acquisition. Test and laboratory facilities can potentially have even greater potential negative consequences to a program than M&S if there are errors present in the test and analysis results, since test results are usually considered closer to the "truth" than M&S results. This paper will discuss how the risk-based M&S verification, validation and accreditation (VV&A) process is being applied to test and laboratory facilities, issues associated with this different application of the process, and thoughts on the broader applicability of risk-based VV&A beyond the current application.

  6. GSFC Space Simulation Laboratory Contamination Philosophy: Efficient Space Simulation Chamber Cleaning Techniques

    NASA Technical Reports Server (NTRS)

    Roman, Juan A.; Stitt, George F.; Roman, Felix R.

    1997-01-01

    This paper will provide a general overview of the molecular contamination philosophy of the Space Simulation Test Engineering Section and how the National Aeronautics and Space Administration (NASA) Goddard Space Flight Center (GSFC) space simulation laboratory controls and maintains the cleanliness of all its facilities, thereby, minimizing down time between tests. It will also briefly cover the proper selection and safety precautions needed when using some chemical solvents for wiping, washing, or spraying thermal shrouds when molecular contaminants increase to unacceptable background levels.

  7. Personnel techniques necessary to maximize bio-barrier integrity at a Martian receiving laboratory

    NASA Technical Reports Server (NTRS)

    Michaelson, G. S.; Mahoney, T. A.

    1975-01-01

    The planning of biological isolation measures for the Mars Surface Sample Return Mission is discussed in terms of personnel and organizational management. Deficiencies in past operation of the Lunar Receiving Laborator are analyzed. It was found that the failure to clearly define relationship among the government agencies involved and to effectively integrate their objectives and responsibilities was a major cause of Laboratory deficiencies. Possible solutions to these problems are presented for application to future missions.

  8. 5. View West. West side and rear elevations of c. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. View West. West side and rear elevations of c. 1890 first rear addition; partial north rear elevation of c. 1900 side ell addition; and north rear and west side elevation of final rear addition of c. 1940. - Vaughn Chevrolet Building, 101-109 East Main Street, Monongahela, Washington County, PA

  9. Experience with Formal Methods techniques at the Jet Propulsion Laboratory from a quality assurance perspective

    NASA Technical Reports Server (NTRS)

    Kelly, John C.; Covington, Rick

    1993-01-01

    Recent experience with Formal Methods (FM) in the Software Quality Assurance Section at the Jet Propulsion Lab is presented. An integrated Formal Method process is presented to show how related existing requirements analysis and FM techniques complement one another. Example application of FM techniques such as formal specifications and specification animators are presented. The authors suggest that the quality assurance organization is a natural home for the Formal Methods specialist, whose expertise can then be used to best advantage across a range of projects.

  10. Characterisation of NO production and consumption: new insights by an improved laboratory dynamic chamber technique

    NASA Astrophysics Data System (ADS)

    Behrendt, T.; Veres, P. R.; Ashuri, F.; Song, G.; Flanz, M.; Mamtimin, B.; Bruse, M.; Williams, J.; Meixner, F. X.

    2014-10-01

    Biogenic NOx emissions from natural and anthropogenically influenced soils are currently estimated to amount to 9 Tg a-1, hence a significant fraction of global NOx emissions (45 Tg a-1). During the last three decades, a large number of field measurements have been performed to quantify biogenic NO emissions. To study biogenic NO emissions as a function of soil moisture, soil temperature, and soil nutrients, several laboratory approaches have been developed to estimate local/regional NO emissions by suitable upscaling. This study presents an improved and automated laboratory dynamic chamber system (consisting of six individual soil chambers) for investigation and quantification of all quantities necessary to characterise biogenic NO release from soil (i.e. net NO release rate, NO production and consumption rate, and respective Q10 values). In contrast to former versions of the laboratory dynamic chamber system, the four experiments for complete characterisation can now be performed on a single soil sample, whereas former studies had to be performed on four sub-samples. This study discovered that the sub-sample variability biased former measurements of net NO release rates tremendously. Furthermore, it was also shown that the previously reported variation of optimum soil moisture (i.e. where a maximum net NO release rates occur) between individual sub-samples is most likely a methodical artefact of former versions of the laboratory dynamic chamber system. A comprehensive and detailed methodical concept description of the improved laboratory dynamic chamber system is provided. Response of all quantities (necessary to characterise net NO release) to soil temperature and NO mixing ratio of the flushing airstream are determined by automatic monitoring of these variables during one single drying-out experiment with one single soil sample only. The method requires precise measurements of NO mixing ratio at the inlet and outlet of each soil chamber; finally, four pairs of inlet/outlet NO mixing ratios are sufficient to derive all necessary quantities. Soil samples from drylands exhibit particularly low NO production, but even lower NO consumption rates. However, with the improved laboratory dynamic chamber system those low levels can be quantified, as well as corresponding NO compensation point mixing ratios and respective Q10 values. It could be shown that the NO compensation point mixing ratio seems to be generally independent of gravimetric soil moisture content, but, particularly for dryland soils, strongly dependent on soil temperature. New facilities have been included into the improved system (e.g. for investigation of net release rates of other trace gases, namely CO2 and volatile organic compounds - VOCs). First, results are shown for net release rates of acetone (C3H6O), acetaldehyde (C2H4O) and CO2. This new system is thus able to simultaneously investigate potential mechanistic links between NO, multitudinous VOC and CO2.

  11. A rapid manual processing technique for resource-limited small laboratories

    PubMed Central

    Jali, Pramod K; Donoghue, Mandana; Gadiwan, Mohsin

    2015-01-01

    Background: Surgical pathology is an integral part of diagnosis and management planning in patient care. In the absence of widespread automation, many small laboratories are unable to provide this service due to lack of time. Currently, the shortest processing schedule for formalin-fixed paraffin-embedded tissues is 16 working hours; most small laboratories cannot complete the rapid schedules within the average 8–9 working hours. Thus, the availability of an 8–9 h processing schedule that provide satisfactory results can help many small laboratories in routinely providing surgical pathology services. Objectives: To evaluate the effectiveness of a new rapid processing schedule and compare it with two existing rapid processing schedules. Materials and Methods: This animal study tested a new rapid processing schedule suggested by authors with overnight preprocessing in 60% isopropyl alcohol followed by an 8 h processing schedule. This was tested and compared with the rapid processing schedules described by Godkar's (11 h) and Bancroft's (2 working days). A routinely used automatic tissue processor long cycle (17 h) was the control. Each schedule was used on 20 tongue specimens. The prepared slides were evaluated for surface area and linear tissue shrinkage, ease of sectioning, quality of hematoxylin and eosin staining, histological appearance and artifacts. Results: No statistical differences were found between schedules. Overall total average performance ranking placed Bancroft's schedule as the best with only 27.9% of the sections processed and stained showing any shortcomings, followed closely by the test schedule suggested by authors (28.7%), Godkar's (31.8%) and the automatic processor schedule/control (33.3%). Conclusion: The test results indicated that the schedule devised by authors is an effective rapid processing cycle that produces diagnostic quality histological results when compared with other conventional processing schedules for small tissue blocks (average 6 mm × 8 mm).

  12. Laboratory procedures and data reduction techniques to determine rheologic properties of mass flows

    USGS Publications Warehouse

    Holmes, R.R., Jr.; Huizinga, R.J.; Brown, S.M.; Jobson, H.E.

    1993-01-01

    Determining the rheologic properties of coarse- grained mass flows is an important step to mathematically simulate potential inundation zones. Using the vertically rotating flume designed and built by the U.S. Geological Survey, laboratory procedures and subsequent data reduction have been developed to estimate shear stresses and strain rates of various flow materials. Although direct measurement of shear stress and strain rate currently (1992) are not possible in the vertically rotating flume, methods were derived to estimate these values from measurements of flow geometry, surface velocity, and flume velocity.

  13. Low-cost foil metallization using arc discharge for passivated emitter and rear solar cells

    NASA Astrophysics Data System (ADS)

    Kurimoto, Yuji; Yamasaki, Ichiro

    2016-04-01

    For the cost reduction of passivated emitter and rear cells (PERC), we propose a new rear contact formation method, in which an aluminum foil and an arc discharge system are used. The arc discharge system consists of inexpensive parts and does not contain any sophisticated part such as a laser ablation apparatus. Therefore, this system can save the cost of the rear contact forming process. We applied this technique to a test production of PERC. It is found that the arc discharge system can provide a similar performance to that attained by a conventional PERC production method.

  14. A Practical Introduction to Separation and Purification Techniques for the Beginning Organic Chemistry Laboratory.

    ERIC Educational Resources Information Center

    Leonard, Jack E.

    1981-01-01

    Describes a sequence of experiments developed at Texas A&M University for use in one-semester and two-semester (nonmajors) organic chemistry courses to teach a maximum number of separation and purification techniques such as distillations, recrystallization, liquid-liquid extraction, and chromatography. (SK)

  15. ADAPTING WOODY SPECIES AND PLANTING TECHNIQUES TO LANDFILL CONDITIONS, FIELD AND LABORATORY INVESTIGATIONS

    EPA Science Inventory

    A study was undertaken to determine which tree species can best maintain themselves in a landfill environment; to investigate the feasibility of preventing landfill gas from penetrating the root zone of selected species by using gas-barrier techniques; and to identify the (those)...

  16. A laboratory system for the investigation of rain fade compensation techniques for Ka-band satellites

    NASA Technical Reports Server (NTRS)

    Svoboda, James S.; Kachmar, Brian A.

    1993-01-01

    The design and performance of a rain fade simulation/counteraction system on a laboratory simulated 30/20 GHz, time division multiple access (TDMA) satellite communications testbed is evaluated. Severe rain attenuation of electromagnetic radiation at 30/20 GHz occurs due to the carrier wavelength approaching the water droplet size. Rain in the downlink path lowers the signal power present at the receiver, resulting in a higher number of bit errors induced in the digital ground terminal. The laboratory simulation performed at NASA Lewis Research Center uses a programmable PIN diode attenuator to simulate 20 GHz satellite downlink geographic rain fade profiles. A computer based network control system monitors the downlink power and informs the network of any power threshold violations, which then prompts the network to issue commands that temporarily increase the gain of the satellite based traveling wave tube (TWT) amplifier. After the rain subsides, the network returns the TWT to the normal energy conserving power mode. Bit error rate (BER) data taken at the receiving ground terminal serves as a measure of the severity of rain degradation, and also evaluates the extent to which the network can improve the faded channel.

  17. A laboratory system for the investigation of rain fade compensation techniques for Ka-band satellites

    NASA Astrophysics Data System (ADS)

    Svoboda, James S.; Kachmar, Brian A.

    1993-12-01

    The design and performance of a rain fade simulation/counteraction system on a laboratory simulated 30/20 GHz, time division multiple access (TDMA) satellite communications testbed is evaluated. Severe rain attenuation of electromagnetic radiation at 30/20 GHz occurs due to the carrier wavelength approaching the water droplet size. Rain in the downlink path lowers the signal power present at the receiver, resulting in a higher number of bit errors induced in the digital ground terminal. The laboratory simulation performed at NASA Lewis Research Center uses a programmable PIN diode attenuator to simulate 20 GHz satellite downlink geographic rain fade profiles. A computer based network control system monitors the downlink power and informs the network of any power threshold violations, which then prompts the network to issue commands that temporarily increase the gain of the satellite based traveling wave tube (TWT) amplifier. After the rain subsides, the network returns the TWT to the normal energy conserving power mode. Bit error rate (BER) data taken at the receiving ground terminal serves as a measure of the severity of rain degradation, and also evaluates the extent to which the network can improve the faded channel.

  18. MASS REARING CODLING MOTHS: IMPROVEMENTS AND MODIFICATIONS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Modifications of the diet, oviposition cages, rearing containers, diapause induction and adult handling are described for a rearing colony of codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), maintained at the USDA-ARS facility in Wapato, Washington (USA), for over 40 years for use in f...

  19. Rear-facing car seat (image)

    MedlinePlus

    A rear-facing car seat position is recommended for a child who is very young. Extreme injury can occur in an accident because ... child. In a frontal crash a rear-facing car seat is best, because it cradles the head, ...

  20. Family Rearing Antecedents of Pubertal Timing

    ERIC Educational Resources Information Center

    Belsky, Jay; Steinberg, Laurence D.; Houts, Renate M.; Friedman, Sarah L.; DeHart, Ganie; Cauffman, Elizabeth; Roisman, Glenn I.; Halpern-Felsher, Bonnie L.; Susman, Elisabeth

    2007-01-01

    Two general evolutionary hypotheses were tested on 756 White children (397 girls) studied longitudinally: (1) rearing experiences would predict pubertal timing; and (2) children would prove differentially susceptible to rearing. Analysis of pubertal measurements, including some based on repeated physical assessments, showed that mothering and…

  1. Parents and the Dynamics of Child Rearing.

    ERIC Educational Resources Information Center

    Holden, George W.

    This book, designed for advanced courses on parent-child relationships, examines scientific evidence concerning parents' effect on children's development. Chapter 1, "The Development of Child-Rearing Research: From Mere Beliefs to a More Dynamic Perspective," discusses the beginnings of and current trends in child rearing research and introduces a…

  2. Rear Steering Inoperative: Apollo 16 Mission

    NASA Technical Reports Server (NTRS)

    1973-01-01

    The inoperative condition of the rear steering system of the lunar roving vehicle (Apollo 16) during the initial drive to the modular equipment stowage assembly was investigated. The front and rear steering systems are described, and the failure analyzed. It is concluded that an open circuit must have occurred either in the hand controller potentiometer or between the potentiometer wiper and the summing node.

  3. Health evaluation of free-ranging and hand-reared macaws (Ara spp.) in Peru.

    PubMed

    Karesh, W B; del Campo, A; Braselton, W E; Puche, H; Cook, R A

    1997-12-01

    As part of ongoing ecological studies and reproduction enhancement efforts for macaws in southwestern Peru, a health survey of parent- and hand-reared scarlet macaws (Ara macao) and blue and gold macaws (Ara ararauna) was conducted in 1994. Thirty-three birds were examined during handling procedures, and blood samples were collected from 27 (9 parent reared, 18 hand reared) for laboratory analysis. All but one bird appeared to be in good condition, with no abnormality noted during physical examination. Hematology, plasma chemistries, and plasma vitamin and mineral levels were studied and correlated with the results of bacterial and viral serology. Positive antibody titers for Salmonella and psittacine herpesvirus were found. These diseases have the potential to affect wildlife population dynamics, and Salmonella may have public health significance. Serological tests for avian influenza, infectious laryngotracheitis, paramyxovirus-1, -2, -3, polyoma virus, chlamydiosis, and aspergillosis were negative. Differences in disease prevalence were found between rearing situations. PMID:9523629

  4. Laboratory analysis of techniques for remote sensing of estuarine parameters using laser excitation

    NASA Technical Reports Server (NTRS)

    Exton, R. J.; Houghton, W. M.; Esaias, W.; Harriss, R. C.; Farmer, F. H.; White, H. H.

    1983-01-01

    The theoretical concepts underlying remote sensing of estuarine parameters using laser excitation are examined. The concepts are extended to include Mie scattering as a measure of the total suspended solids and to develop the water Raman signal as an internal standard. Experimental validation of the theory was performed using backscattered laser light from a laboratory tank to simulate a remote-sensing geometry. Artificially prepared sediments and biological cultures were employed to check specific aspects of the theory under controlled conditions. Natural samples gathered from a variety of water types were also analyzed in the tank to further enhance the simulation. The results indicate that it should be possible to remotely quantify total suspended solids, dissolved organics, attenuation coefficient, chlorophyll a, and phycoerythrin in estuarine water using laser excitation.

  5. Application of non-intrusive geophysical techniques at the Mixed Waste Landfill, Technical Area 3, Sandia National Laboratories, New Mexico

    SciTech Connect

    Peace, J.L.; Hyndman, D.A.; Goering, T.J.

    1996-03-01

    The Environmental Restoration Project at Sandia National Laboratories, New Mexico is tasked with assessment and remediation of the Mixed Waste Landfill in Technical Area 3. The Mixed Waste Landfill is an inactive radioactive and mixed waste disposal site. The landfill contains disposal pits and trenches of questionable location and dimension. Non-intrusive geophysical techniques were utilized to provide an effective means of determining the location and dimension of suspected waste disposal trenches before Resource Conservation and Recovery Act intrusive assessment activities were initiated. Geophysical instruments selected for this investigation included a Geonics EM-31 ground conductivity meter, the new Geonics EM-61 high precision, time-domain metal detector, and a Geometrics 856 total field magnetometer. The results of these non-intrusive geophysical techniques were evaluated to enhance the efficiency and cost-effectiveness of future waste-site investigations at Environmental Restoration Project sites.

  6. Hand rearing affects emotional responses but not basic cognitive performance in European starlings.

    PubMed

    Feenders, Gesa; Bateson, Melissa

    2013-07-01

    Hand rearing is a common procedure in behavioural research on birds. While likely to produce tamer experimental animals, there is a risk that it could induce pathological changes in brain and behaviour similar to those seen in mammals that have experienced maternal separation. We explored the effects of hand rearing on the cognitive and behavioural development of European starlings, Sturnus vulgaris, to assess the generality of results obtained from hand-reared animals. Two groups of age-matched birds were created from the same wild population: one hand-reared from 10 days posthatch and one brought into the laboratory as independent juveniles. These groups were compared on a battery of neuropsychological tasks designed to probe different aspects of cognitive function including learning, perseverative cognition, interval timing, neophobia and impulsivity. There was no evidence for cognitive impairment in the hand-reared birds. They did not have reduced learning speed, impairments in accuracy or precision of interval timing or pathological perseverative cognition compared to the wild-caught birds. Additionally, there was no evidence that birds that developed stereotypies in laboratory cages (predominantly the wild-caught birds) had any cognitive impairments, although this may be because no birds had severe, crystallized stereotypies. There was some evidence that hand-reared birds were less neophobic and less impulsive than wild-caught birds, suggesting that hand rearing might alter emotionally mediated decision making in a direction usually associated with reduced developmental stress in mammals. This study therefore supports the use of hand rearing as an experimental procedure in behavioural research on passerine birds. PMID:23888084

  7. Hand rearing affects emotional responses but not basic cognitive performance in European starlings☆

    PubMed Central

    Feenders, Gesa; Bateson, Melissa

    2013-01-01

    Hand rearing is a common procedure in behavioural research on birds. While likely to produce tamer experimental animals, there is a risk that it could induce pathological changes in brain and behaviour similar to those seen in mammals that have experienced maternal separation. We explored the effects of hand rearing on the cognitive and behavioural development of European starlings, Sturnus vulgaris, to assess the generality of results obtained from hand-reared animals. Two groups of age-matched birds were created from the same wild population: one hand-reared from 10 days posthatch and one brought into the laboratory as independent juveniles. These groups were compared on a battery of neuropsychological tasks designed to probe different aspects of cognitive function including learning, perseverative cognition, interval timing, neophobia and impulsivity. There was no evidence for cognitive impairment in the hand-reared birds. They did not have reduced learning speed, impairments in accuracy or precision of interval timing or pathological perseverative cognition compared to the wild-caught birds. Additionally, there was no evidence that birds that developed stereotypies in laboratory cages (predominantly the wild-caught birds) had any cognitive impairments, although this may be because no birds had severe, crystallized stereotypies. There was some evidence that hand-reared birds were less neophobic and less impulsive than wild-caught birds, suggesting that hand rearing might alter emotionally mediated decision making in a direction usually associated with reduced developmental stress in mammals. This study therefore supports the use of hand rearing as an experimental procedure in behavioural research on passerine birds. PMID:23888084

  8. Comparison between ELISA and various stains techniques in laboratory diagnosis of cryptosporidiosis.

    PubMed

    Gabr, Nabil S; Abdellatif, Manal Z M; Abd El-Hafeez, Ekhlas; Abd Rabou, Reham A M

    2014-08-01

    Cryptosporidium spp. is an important parasitic protozoa causing diarrhea which is a severe life-threatening diarrhea especially in immunocompromised hosts. We aimed to evaluate the usefulness of detection of Cryptosporidium spp. copro-antigen from fecal specimens by using enzyme linked immunosorbent assay (ELISA) test and comparing its sensitivity and specificity with some staining methods. The results revealed that Modified Acid-Fast stain is considered better than Giemsa in detecting Cryptosporidium species oocysts in faecal smears as their sensitivity were 67.5% and 53.75% respectively. On contrary, ELISA technique is considered the best method used for detection of cryptosporidial infection as its sensitivity is 90%. PMID:25597165

  9. Assessment of body fatness in childhood obesity: evaluation of laboratory and anthropometric techniques.

    PubMed

    Bandini, L G; Dietz, W H

    1987-10-01

    The identification of obesity as a pathological diagnosis depends on an accurate assessment of body fatness and a correlation of fatness with pathological consequences. Because total body fat varies with body weight, the proportion of body weight that is fat is probably a more reliable indicator of risk. Among obese children and adolescents, several problems have hindered the development of accurate clinical measures of percent body fat and total body fat. First, the use of direct methods to measure body composition is limited by expense and labor. Second, the relationship between anthropometric indexes and body composition in obese children and adolescents has not been intensively studied. Third, sample sizes of normal weight children have been too small to permit the development of diagnostic criteria. Fourth, the triceps skinfold is less reproducible in overweight subjects. Increases in lean body mass in obese adolescents may confound the use of the body mass index as a measure of adiposity. Current laboratory methods for the measurement of body composition include: (1) underwater weighing, (2) 40K counting, (3) isotopic dilution measures, (4) neutron activation, and (5) electrical impedance. This article examines relationships between those methods and anthropometry in the measurement of fatness in children and adolescents, as well as the difficulties in measuring body fatness and the importance of body fat distribution and its relationship to morbidity in children. Current evidence suggests an association of morbidity and upper segment obesity in adults. Corresponding studies in children and adolescents are yet to be carried out. PMID:3655163

  10. Assessment of body fatness in childhood obesity: evaluation of laboratory and anthropometric techniques

    SciTech Connect

    Bandini, L.G.; Dietz, W.H. Jr.

    1987-10-01

    The identification of obesity as a pathological diagnosis depends on an accurate assessment of body fatness and a correlation of fatness with pathological consequences. Because total body fat varies with body weight, the proportion of body weight that is fat is probably a more reliable indicator of risk. Among obese children and adolescents, several problems have hindered the development of accurate clinical measures of percent body fat and total body fat. First, the use of direct methods to measure body composition is limited by expense and labor. Second, the relationship between anthropometric indexes and body composition in obese children and adolescents has not been intensively studied. Third, sample sizes of normal weight children have been too small to permit the development of diagnostic criteria. Fourth, the triceps skinfold is less reproducible in overweight subjects. Increases in lean body mass in obese adolescents may confound the use of the body mass index as a measure of adiposity. Current laboratory methods for the measurement of body composition include: (1) underwater weighing, (2) 40K counting, (3) isotopic dilution measures, (4) neutron activation, and (5) electrical impedance. This article examines relationships between those methods and anthropometry in the measurement of fatness in children and adolescents, as well as the difficulties in measuring body fatness and the importance of body fat distribution and its relationship to morbidity in children. Current evidence suggests an association of morbidity and upper segment obesity in adults. Corresponding studies in children and adolescents are yet to be carried out.

  11. Response properties of area 17 neurons in cats reared in stroboscopic illumination.

    PubMed

    Cremieux, J; Orban, G A; Duysens, J; Amblard, B

    1987-05-01

    The response properties of 196 area 17 cells were studied qualitatively in seven cats reared from birth in a stroboscopically illuminated environment (frequency, 2/s; duration, 200 microseconds). Quantitative testing with the multihistogram technique was carried out in 115 cells. As control population, 453 neurons recorded in area 17 of the normal adult cat and tested qualitatively (of which 301 neurons were tested quantitatively) were available. In area 17 of strobe-reared cats, a number of spatial characteristics of receptive fields investigated with hand-held stimuli were found to be abnormal. There was a strong reduction in the encounter frequency both of end-stopped cells and of binocularly driven cells in the strobe-reared cats. Central receptive fields in strobe-reared cats were wider than in normal cats, but the increase in receptive-field width with eccentricity was still observed. More cells than in normal cats showed either no selectivity or only a weak bias for stimulus orientation, but the orientation tuning of orientation-selective cells was similar in strobe-reared and normal cats. Quantitative testing revealed that the velocity preference of cells in area 17 subserving central vision was different in strobe-reared cats from that of normal cats, due to a reduction in the encounter frequency of cells showing a preference for low velocities. There was no difference in velocity preference between strobe-reared and normal cats in the parts of area 17 that subserve peripheral vision, the proportion of neurons responding to fast velocities showing a similar increase in both groups of animals. Fewer cells were direction selective in strobe-reared cats than in normal cats. Most of the remaining direction-selective cells had peripheral receptive fields and the synergism between leaving an OFF subregion and entering an ON subregion contributed to their direction selectivity. Latency of neurons in area 17 of strobe-reared cats was slightly higher than in normal cats, but the response strength of neurons was the same in the two groups. The proportion of cells failing to respond to briefly flashed stationary stimuli was significantly lower in strobe-reared than in normal animals. Qualitative and quantitative testing showed that strobe rearing has a stronger effect on the parts of area 17 that subserve central vision than on those that subserve peripheral vision. Comparing the present results with those of Kennedy and Orban (37) shows that strobe rearing has less effect on area 17 than on area 18 and that the functional differences between areas 17 and 18 in strobe-reared cats are smaller than in normal cats. PMID:3585478

  12. Laboratory techniques for determining the effects of pyridostigmine bromide. Interim report, May 1985-May 1986

    SciTech Connect

    Parker, F.R.; Barber, J.A.; Forster, E.M.; Whinnery, J.E.

    1986-11-01

    The USAF Surgeon General approved pyridostigmine bromide (PB) pretreatment for flying personnel as of 21 March 1986. However, specific side effects resulting from taking PB during high sustained + G/sub z/ stress, altitude stress, and periods of potential spatial disorientation had not yet been fully investigated. The USAF school of Aerospace Medicine (UASFSAM), Crew Technology Division, was therefore tasked with this clinical research effort. For effective investigation of these potential problem areas, rapid and accurate methods have had to be established for determining the blood plasma PB levels and the resultant inhibition of acetylcholinesterase activity. The basic purpose of this report is to describe in detail the analytical techniques used at USAFSAM to support the clinical research programs involving the physiological and performance aspects of USAF aircrews taking PB.

  13. Possible signatures of dissipation from time-series analysis techniques using a turbulent laboratory magnetohydrodynamic plasma

    NASA Astrophysics Data System (ADS)

    Schaffner, D. A.; Brown, M. R.; Rock, A. B.

    2016-05-01

    The frequency spectrum of magnetic fluctuations as measured on the Swarthmore Spheromak Experiment is broadband and exhibits a nearly Kolmogorov 5/3 scaling. It features a steepening region which is indicative of dissipation of magnetic fluctuation energy similar to that observed in fluid and magnetohydrodynamic turbulence systems. Two non-spectrum based time-series analysis techniques are implemented on this data set in order to seek other possible signatures of turbulent dissipation beyond just the steepening of fluctuation spectra. Presented here are results for the flatness, permutation entropy, and statistical complexity, each of which exhibits a particular character at spectral steepening scales which can then be compared to the behavior of the frequency spectrum.

  14. Laboratory Techniques in Geology: Embedding Analytical Methods into the Undergraduate Curriculum

    NASA Astrophysics Data System (ADS)

    Baedke, S. J.; Johnson, E. A.; Kearns, L. E.; Mazza, S. E.; Gazel, E.

    2014-12-01

    Paid summer REU experiences successfully engage undergraduate students in research and encourage them to continue to graduate school and scientific careers. However these programs only accommodate a limited number of students due to funding constraints, faculty time commitments, and limited access to needed instrumentation. At JMU, the Department of Geology and Environmental Science has embedded undergraduate research into the curriculum. Each student fulfilling a BS in Geology or a BA in Earth Science completes 3 credits of research, including a 1-credit course on scientific communication, 2 credits of research or internship, followed by a presentation of that research. Our department has successfully acquired many analytical instruments and now has an XRD, SEM/EDS, FTIR, handheld Raman, AA, ion chromatograph, and an IRMS. To give as many students as possible an overview to the scientific uses and operation methods for these instruments, we revived a laboratory methods course that includes theory and practical use of instrumentation at JMU, plus XRF sample preparation and analysis training at Virginia Tech during a 1-day field trip. In addition to practical training, projects included analytical concepts such as evaluating analytical vs. natural uncertainty, determining error on multiple measurements, signal-to-noise ratio, and evaluating data quality. State funding through the 4-VA program helped pay for analytical supplies and support for students to complete research projects over the summer or during the next academic year using instrumentation from the course. This course exemplifies an alternative path to broadening participation in undergraduate research and creating stronger partnerships between PUI's and research universities.

  15. PCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinic

    PubMed Central

    Martinez, Alejandra Nóbrega; Talhari, Carolina; Moraes, Milton Ozório; Talhari, Sinésio

    2014-01-01

    In leprosy, classic diagnostic tools based on bacillary counts and histopathology have been facing hurdles, especially in distinguishing latent infection from active disease and diagnosing paucibacillary clinical forms. Serological tests and IFN-gamma releasing assays (IGRA) that employ humoral and cellular immune parameters, respectively, are also being used, but recent results indicate that quantitative PCR (qPCR) is a key technique due to its higher sensitivity and specificity. In fact, advances concerning the structure and function of the Mycobacterium leprae genome led to the development of specific PCR-based gene amplification assays for leprosy diagnosis and monitoring of household contacts. Also, based on the validation of point-of-care technologies for M. tuberculosis DNA detection, it is clear that the same advantages of rapid DNA detection could be observed in respect to leprosy. So far, PCR has proven useful in the determination of transmission routes, M. leprae viability, and drug resistance in leprosy. However, PCR has been ascertained to be especially valuable in diagnosing difficult cases like pure neural leprosy (PNL), paucibacillary (PB), and patients with atypical clinical presentation and histopathological features compatible with leprosy. Also, the detection of M. leprae DNA in different samples of the household contacts of leprosy patients is very promising. Although a positive PCR result is not sufficient to establish a causal relationship with disease outcome, quantitation provided by qPCR is clearly capable of indicating increased risk of developing the disease and could alert clinicians to follow these contacts more closely or even define rules for chemoprophylaxis. PMID:24722358

  16. Experimental Evaluation of Multi-spacecraft Data Analysis Techniques in a Laboratory Plasma

    SciTech Connect

    Jongsoo Yoo and Masaaki Yamada

    2012-03-27

    The Magnetic Reconnection Experiment (MRX)[1] has been utilized to assess the effectiveness of minimum variance analysis on the magnetic field (MVAB) and boundary-crossing time analysis (BCTA). The neutral sheet is swept, or jogged, in a controlled manner with respect to the stationary probes by pulsed internal coil currents. Magnetic field data from measurement points resembling data from multi-spacecraft flying though a reconnecting current sheet is used to check both techniques to deduce a proper normal vector. We examine discharges with the two-dimensional (2-D) X-line structure as well as cases in which a flux rope forms within the layer. All discharges are in a two-fluid regime in which electrons are magnetized but not ions. Boundary-crossing time analysis with four sample measurement points forming a tetrahedron generates a reasonable unit normal vector and relative velocity along the normal vector for all of the tested cases. On the other hand, MVAB sometimes fails to predict a proper normal direction. This is because the X-line magnetic geometry is fundamentally 2-D or 3-D. However, the direction along the reconnecting field determined by MVAB does not deviate much from the real magnetic geometry documented by 2-D magnetic probe arrays and one additional probe at a different toroidal location. Based on these observations, we suggest a procedure for determining a local coordinate system for data from the Magnetospheric Multi-Scale (MMS) mission when spacecraft passes through a reconnecting current sheet. The distance between measurement points on the order of the ion skin depth (c/{omega}{sub pi}) is pertinent to determination of the magnetic geometry.

  17. Segment inertial properties of primates: new techniques for laboratory and field studies of locomotion.

    PubMed

    Crompton, R H; Li, Y; Alexander, R M; Wang, W; Gunther, M M

    1996-04-01

    Studies of the dynamics of locomotor performances depend on knowledge of the distribution of body mass within and between limb segments. However, these data are difficult to derive. Segment mass properties have generally been estimated by modelling limbs as truncated cones, but this approach fails to take into account that some segments are of elliptical, not circular, cross section; and further, the profiles of real segments are generally curved. Thus, they are more appropriately modelled as solids of revolution, described by the rotation in space of convex or concave curves, and the possibility of an elliptical cross section needs to be taken into account. In this project we have set out to develop a general geometric model which can take these factors into account, and permit segment inertial properties to be derived from cadavers by segmentation, and from living individuals using linear external measurements. We present a model which may be described by up to four parameters, depending on the profile and serial cross section (circular or ellipsoidal) of the individual segments. The parameters are obtained from cadavers using a simplified complex-pendulum technique, and from intact specimens by calculation from measurements of segment diameters and lengths. From the parameters, the center of mass, moments of interia, and radii of gyration may be derived, using simultaneous equations. Inertial properties of the body segments of four Pan troglodytes and a single Pongo were determined, and contrasted to comparable findings for humans. Using our approach, the mass distribution characteristics of any individual or species may be represented by a rigid-link segment model or "android." If this is made to move according to motion functions derived from a real performance of the individual represented, we show that recordings of resulting ground reaction forces may be quite closely simulated by predictive dynamic modelling. PMID:8779338

  18. Laboratory two-dimensional X-ray microdiffraction technique: a support for authentication of an unknown Ghirlandaio painting

    NASA Astrophysics Data System (ADS)

    Bontempi, E.; Benedetti, D.; Massardi, A.; Zacco, A.; Borgese, L.; Depero, L. E.

    2008-07-01

    Europe has a very rich and diversified cultural heritage of art works, including buildings, monuments and objects of all sizes, involving a great variety of materials. The continuous discovery of new art works opens the problem of their authentication. Advanced analytical techniques can be fundamental to understand the way of life, the culture and the technical and intellectual know-how of the artists. Indeed, the authentication of an art work involves the identification of the used materials, their production techniques and procedures used for the work realization. It is possible to know the origin and provenance of materials, including the location of the natural sources. Advanced analytical techniques also help one to understand degradation processes, corrosion, weathering, and preservation-conservation protocols. In this paper we present a painting attributed to Domenico Ghirlandaio. Ghirlandaio is a well-known artist of fifteenth century who contributes to the apprenticeship of Michelangelo Buonarroti. The study of the pigments used in this painting, which belongs to a private collection, has been supported mainly by means of laboratory two-dimensional X-ray microdiffraction (μXRD2). The possibility to obtain information about not only the phase, but also microstructure allows one to extract interesting consideration and to obtain evidence of the painter’s style and intention.

  19. Laboratory studies of peroxy radical reactions using the turbulent flow chemical ionisation mass spectrometer technique

    NASA Astrophysics Data System (ADS)

    Bacak, A.; Bardwell, M.; Percival, C.

    2003-04-01

    HO_x (sum of OH and HO_2) plays an important role in the formation of tropospheric O_3 recently Wennberg et al.,^1 pointed out that the formation of tropospheric O_3 is strongly dependent on the level of NO_x as a consequence of its reaction with RO_2 (where R=H or CH_3). It is therefore important to characterise the coupling of NO_x and RO_2 to understand the tropospheric O_3 budget. Model studies have shown in the mid to upper troposphere both HO_2NO_2 and CH_3O_2NO_2 may well constitute a significant fraction of NO_y (˜20%). Despite the importance of these species, there are little or no experimental data available on their formation in this region. At present models do not accurately describe the observed nitrogen partitioning, in particular underpredicting the ratio NO_x/NO_y Recently Brown et al.,^2 have measured key rate coefficients over a wider range of pressure and temperature than performed previously and have shown that the formation of NO_y is much slower than previously estimated from extrapolation of available kinetic data to low temperatures Gao et al.,^3 have observed that these new kinetic data improve the agreement between model and measurements in the lower stratosphere and troposphere respectively, but that discrepancies still exist which must be addressed. The temperatures (180-300 K) encountered in the troposphere pose a significant challenge to laboratory studies. Although much progress has been made in this respect over the years, there still remains considerable uncertainties in the kinetic data base, particularly for conditions of lowest temperature (180 - 250 K) and pressures (70 Torr) that pertain to the UTLS region. A turbulent flow CIMS (as shown in figure 1) has been developed to study the reaction of RO_2 with NO over the pressure range 70-760 Torr and temperatures as low as 170 K. The CIMS is used to detect all trace species in the flow tube. Chemical ionisation takes place through the following reaction scheme. X^- + RO_2 longrightarrow RO_2^- + X 210Po emitting a particles is used to ionise N_2 used as a carrier gas, thus creating secondary electrons. These electrons attach rapidly to the neutral precursor X (e.g. SF6) of the donor to produce X^-. The X^- ion can then react via a charge transfer with the RO_2 species of interest. The resultant RO_2^- is then passed to the mouth of a quadrupole-mass filter via a set of ion optics and detected by an ion multiplier. References (1) Wennberg, P.O., T.F. Hanisco, L. Jaegle, D.J. Jacob, E.J. Hintsa, E.J. Lazendorf, J.G. anderson, R.-S. Gao, E.R. Keim, S.G. Donnelly, L.A. Del Negro, D.W. Fahey, S.A. Mckeen, R.J. Salawitch, C.R. Webster, R.D. May, R.L. Herman, H.M. Proffitt, J.J. Margitan, E.L. Atlas, S.M. Schauffler, F. Locke, C.T. McElroy and T.P. Bui, Science, 79, 49 (1999). (2) Brown S.S., R.K. Talukdar and A.R. Ravishankara, Chem. Phys. Lett., 99, 277 (1999). (3) Gao, R.S., D.W. Fahey, L.A. Del negro, S.G. Donnelly, E.R. Keim, J.A. Neuman, E. Teverovskaia, P.O. Wennberg, T.F. Hanisco, E.J. Lazendorf, H.M. Proffitt, J.J. Margitan, J.C. Wilson, J.W. Elkins, R.M. Stimpfle, R.C. Cohen, C.T. McElroy, T.P. Bui, R.J. Salawitch, S.S. Brown, A.R. Ravishankara, R.W. Portmann, M.K.W. Ko D.K. Weisenstein and P.A. Newman, Geophys. Res. Lett., 6, 1153 (1999). (4) Seeley, J.V., J.T. Jayne and M.J. Molina, J. Phys. Chem., 00, 4019 (1996).

  20. Improved Techniques Used at Brookhaven National Laboratory to Package and Dispose of Radioisotope Production Waste Lowers Worker Exposure

    SciTech Connect

    Sullivan, P.

    2003-02-24

    This paper describes the operations that generate Radioisotope Production Waste at Brookhaven National Laboratory (BNL) and the improved techniques used to handle and dispose of this waste. Historically, these wastes have produced high worker exposure during processing, packaging and disposal. The waste is made up of accelerator-produced nuclides of short to mid-length half-lives with a few longer-lived nuclides. However, because radiopharmaceutical research and treatment requires a constant supply of radioisotopes, the waste must be processed and disposed of in a timely manner. Since the waste cannot be stored for long periods of time to allow for adequate decay, engineering processes were implemented to safely handle the waste routinely and with ALARA principles in mind.

  1. Application of the Ta liner technique to produce Ca beams at INFN-Legnaro National Laboratories (INFN-LNL)

    SciTech Connect

    Galatà, A. Sattin, M.; Manzolaro, M.; Martini, D.; Facco, A.; Tinschert, K.; Spaedtke, P.; Lang, R.; Kulevoy, T.

    2014-02-15

    The ECR ion sources are able to produce a wide variety of highly charged metallic ion beams thanks to the development of different techniques (ovens, sputtering, direct insertion, metal ions from volatile compounds (MIVOC)). In the case of the ovens, the sticking of the hot vapors on the surface of the plasma chamber leads to high material consumption rates. For elements like Ca, a tantalum liner inserted inside the chamber can be used to limit this phenomenon. The modeling of temperature distribution inside the chamber with and without the liner was carried out with COMSOL-multiphysics code. Results of simulation and the comparison with experiments performed at INFN-Legnaro National Laboratories with Ca beams are discussed.

  2. Laboratory tests and simulations of pulse shape discrimination (PSD) technique for background reduction in germanium gamma-ray detectors

    NASA Astrophysics Data System (ADS)

    Slassi-Sennou, Said; Boggs, Steven E.; Philhour, Bryon; Campbell, Robert D.; Feffer, Paul T.; Lin, Robert P.; McBride, Steven; Primbsch, Henry J.; Cork, Chris P.; Goulding, Fred S.; Luke, Paul N.; Madden, Norman W.; Malone, Don F.; Pehl, Richard H.

    1996-10-01

    For actively shielded, narrow aperture germanium spectrometers at balloon or spacecraft altitudes, the beta decay of radio-active nuclei is the dominant source of background in the 0.2 to 2 MeV energy range. This component of the background is internal to the germanium detectors (GeDs) and results from the activation of Ge nuclei by cosmic ray secondaries. The sensitivity of GeD spectrometers can be improved by rejecting beta-decay events, which deposit energy at a single site in the detector, while retaining photon events, which are predominantly multiple site at these energies. Pulse shape discrimination (PSD) techniques can distinguish between single- and multiple-site events by analyzing the shape of GeDs' current pulses. Here we present results of laboratory tests of PSD with a newly developed narrow-inner-bore (0.6 cm diameter) coaxial GeD and compare them to numerical simulations.

  3. A three dimensional Green's function solution technique for the transport of heavy ions in laboratory and space

    NASA Astrophysics Data System (ADS)

    Gerstner, Candice Rockell

    In the future, astronauts will be sent into space for longer durations of time compared to previous missions. The increased risk of exposure to ionizing radiation, such as Galactic Cosmic Rays and Solar Particle Events, is of great concern. Consequently, steps must be taken to ensure astronaut safety by providing adequate shielding. The shielding and exposure of space travelers is controlled by the transport properties of the radiation through the spacecraft, its onboard systems and the bodies of the individuals themselves. Meeting the challenge of future space programs will therefore require accurate and efficient methods for performing radiation transport calculations to analyze and predict shielding requirements. One such method, which is developed in this dissertation, is based on a three dimensional Green's function solution technique for the transport of heavy ions in both laboratory and space.

  4. Effects of training experienced teachers in the use of the one-minute preceptor technique in the gross anatomy laboratory.

    PubMed

    Chan, Lap Ki; Sharma, Neel

    2014-01-01

    The one-minute preceptor (OMP) is a time-efficient, learner-centered teaching method used in a busy ambulatory care setting. This project evaluated the effects of training experienced anatomy teachers in the use of the OMP in the gross anatomy laboratory on students' perceived learning. Second-year medical students from a five-year, undergraduate-entry, system- and problem-based medical program were divided randomly into two groups of 76 students each. The groups took part in the same gross anatomy laboratory session on different dates, supervised by the same two teachers (both with over 25 years of teaching experience). The teachers attended a workshop on the use of the OMP between the two sessions. Students were given a questionnaire at the end of the two sessions to indicate their agreements to statements regarding their learning experiences. Semistructured interviews were conducted with the two teachers after the second session. Results showed that training experienced anatomy teachers in the use of the OMP did not result in improvement of student learning perception in the gross anatomy laboratory. The experienced teachers have developed their own approaches with elements similar to those in the OMP: being learner centered and adaptable to individual student's needs, providing feedback, and enhancing teacher immediacy. They do not have an explicit structure such as the OMP, and are thus flexible and adaptive. Confining the teachers' teaching behaviors to the OMP structure could limit their performance. Although there are theoretical advantages for novice teachers in adopting the OMP technique, these advantages still need to be supported by further studies. PMID:23864286

  5. Managing Ammonia Emissions From Screwworm Larval Rearing Media.

    PubMed

    Sagel, Agustin; Phillips, Pamela; Chaudhury, Muhammad; Skoda, Steven

    2016-02-01

    Mass production, sterilization, and release of screwworms (Cochliomyia hominivorax (Coquerel)) that were competitive in the field significantly contributed to the successful application of the sterile insect technique for eradication of screwworms from continental North America. Metabolic byproducts resulting from protein-rich diets required for larval screwworms lead to ammonia liberation, sometimes at high levels, within the mass rearing facility. Until recently a sodium polyacrylate gel bulking agent was used for the larval media and adsorbed much of the ammonia. A need to replace the gel with an environmentally "friendly" bulking agent, while not increasing ammonia levels in the rearing facility, led to a series of experiments with the objective of developing procedures to reduce ammonia emissions from the larval media bulked with cellulose fiber. Additives of ammonia-converting bacteria, potassium permanganate, and Yucca schidigera Roezl ex Otrgies powder extract, previously reported to reduce ammonia levels in organic environments, were evaluated. Ammonia-converting bacteria did not have a positive effect. Addition of Y. schidigera powder extract (∼1% of total volume), potassium permanganate (∼250 ppm), and a combination of these two additives (at these same concentrations) kept ammonia at equivalent levels as when larval media was bulked with gel. Potassium permanganate also had sufficient antimicrobial properties that the use of formaldehyde in the diet was not necessary. Further testing is needed, at a mass rearing level, before full implementation into the screwworm eradication program. PMID:26468514

  6. Production and quality assurance in the SIT Africa Mediterranean fruit fly (Diptera: Tephritidae) rearing facility in South Africa

    SciTech Connect

    Barnes, B.; Rosenberg, S.; Arnolds, L.; Johnson, J.

    2007-03-15

    A mass-rearing facility for Mediterranean fruit fly Ceratitis capitata (Wiedemann) was commissioned in Stellenbosch in 1999 to produce sterile male fruit flies for a sterile insect technique (SIT) project in commercial fruit orchards and vineyards in the Western Cape province of South Africa. The mass-rearing procedure was largely based on systems developed by the FAO/IAEA Agriculture and Biotechnology Laboratory, Seibersdorf, Austria. A number of genetic sexing strains were used to produce only males for release. Initial cramped rearing and quality management conditions were alleviated in 2001 with the construction of a new adult rearing room and quality control laboratory. In 2002 a comprehensive Quality Management System was implemented, and in 2003 an improved genetic sexing strain, VIENNA 8, was supplied by the FAO/IAEA Laboratory in Seibersdorf. For most of the first 3 years the facility was unable to supply the required number of sterile male Mediterranean fruit flies for the SIT program without importing sterile male pupae from another facility. From mid-2002, after the quality management system was implemented, both production and quality improved but remained below optimum. After the introduction of the VIENNA 8 genetic sexing strain, and together with an improvement in the climate control equipment, production stability, and quality assurance parameters improved substantially. The critical factors influencing production and quality were an inadequate rearing infrastructure, problems with the quality of the larval diet, and the initial absence of a quality management system. The results highlight the importance of effective quality management, the value of a stable and productive genetic sexing strain, and the necessity for a sound funding base for the mass-rearing facility. (author) [Spanish] La facilidad para criar en masa la mosca mediterranea de la fruta, Ceratitis capitata (Wiedemann) fue comisionada en Stellenbosch en 1999 para producir machos esteriles de moscas para el proyecto de la tecnica del insecto esteril (TIE) en huertos de frutos y vinas comerciales en la provincia del Cabo Occidental del Sudafrica. El procedimiento de criar en masa fue en su mayor parte basado en los sistemas desarrollados por el Laboratorio de Agricultura y Biotecnologia de la FAO/IAEA, Seibersdorf, Austria. Un numero de razas que separara los sexos geneticamente fueron utilizadas para producir solo machos para la liberacion. La congestionada condicion inicial para criar las moscas y su manejo de calidad fueron aliviadas en 2001 con la construccion de un nuevo cuarto de cria para adultos y un laboratorio de control de calidad. En 2002, un Sistema de Manejo de Calidad comprensivo fue implementado, y en 2003 una raza mejorada que separa los sexos geneticamente, VIENNA 8, fue proveido por el Laboratorio de la FAO/IAEA en Seibersdorf. En la mayor parte de los primeros 3 anos la facilidad no pudo suplir el numero requerido de machos esteriles de la mosca mediterranea de la fruta para el programa de TIE sin la necesidad para importar machos esteriles de otra facilidad. Desde medio del ano de 2002, despues que el sistema de manejo de calidad fue implementado, la produccion y la calidad mejoraron pero aun quedaron por debajo del nivel optimo. Despues de la introduccion de la raza VIENNA 8 que separa los sexos geneticamente, y junto con el equipo mejorado de control de clima, la estabilidad y los parametros de seguridad de calidad mejoraron substancialmente. Los factores criticos que influyeron en la produccion y la calidad fueron la infraestructura inadecuada para criar las moscas, problemas con la calidad de la dieta para las larvas y la ausencia inicial de un sistema de manejo de calidad. Los resultados muestran claramente la importancia de un manejo efectivo de la calidad, el valor de una raza productiva que separa los sexos geneticamente y la necesidad de contar con una base solida de financimiento para la infraestructura de una cria en masa. (author)

  7. A comparison of rearing Creontiades signatus distant on green bean pods or pea plants

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A laboratory procedure for mass rearing Creontiades signatus Distant, a relatively new plant bug pest of south Texas cotton, was compared using green bean pods versus whole cowpea plants to determine which would provide for the easiest most efficient method for producing the insect in culture. The n...

  8. Rearing optimization of two races of the Fall armyworm Spodoptera frugiperda feeding natural host plants

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two ecological races of the Fall armyworm Spodoptera frugiperda were raised under laboratory conditions feeding on natural host plants (corn and bermuda grass). Three rearing containers were used: a plastic container and a vertical cylinder to test fitness when feeding gregariously, and individual ...

  9. TV Spots and Supportive Printed Materials on Child-Rearing Practices. Work Unit III. Final Report.

    ERIC Educational Resources Information Center

    Southwest Educational Development Lab., Austin, TX.

    This is the 1976-1977 final report of Work Unit III of Southwest Educational Development Laboratory's Early Childhood Program. The Unit was funded to develop media products, mass media delivery strategies, television spot announcements and supportive booklets designed to increase awareness of certain child-rearing skills and practices among low…

  10. Observational and laboratory studies of optical properties of black and brown carbon particles in the atmosphere using spectroscopic techniques

    NASA Astrophysics Data System (ADS)

    Nakayama, Tomoki; Matsumi, Yutaka

    2015-04-01

    Light absorption and scattering by aerosols are as an important contributor to radiation balance in the atmosphere. Black carbon (BC) is considered to be the most potent light absorbing material in the visible region of the spectrum, although light absorbing organic carbon (brown carbon or BrC) and mineral dust may also act as sources of significant absorption, especially in the ultraviolet (UV) and shorter visible wavelength regions. The optical properties of such particles depend on wavelength, particle size and shape, morphology, coating, and complex refractive index (or chemical composition), and therefore accurate in situ measurements of the wavelength dependence of the optical properties of particles are needed. Recently, cavity ring-down spectroscopy (CRDS) and photoacoustic spectroscopy (PAS) have been used for the direct measurements of extinction and absorption coefficients of particles suspended in air. We have applied these techniques to the observational studies of optical properties of BC and BrC in an urban site in Japan and to the laboratory studies of optical properties of secondary organic aerosols (SOAs) generated from a variety of biogenic and anthropogenic volatile organic compounds and those of diesel exhaust particles (DEPs). In the presentation, the basic principles of these techniques and the results obtained in our studies and in the recent literatures will be overviewed. References Guo, X. et al., Measurement of the light absorbing properties of diesel exhaust particles using a three-wavelength photoacoustic spectrometer, Atmos. Environ., 94, 428-437 (2014). Nakayama, T. et al., Measurements of aerosol optical properties in central Tokyo during summertime using cavity ring-down spectroscopy: Comparison with conventional techniques, Atmos. Environ., 44, 3034-3042 (2010). Nakayama, T. et al., Laboratory studies on optical properties of secondary organic aerosols generated during the photooxidation of toluene and the ozonolysis of alpha-pinene, J. Geophys. Res., 115, D24204, doi:10.1029/2010JD014387 (2010). Nakayama, T. et al., Wavelength dependence of refractive index of secondary organic aerosols generated during the ozonolysis and photooxidation of alpha-pinene, SOLA, 8, 119-124 (2012). Nakayama, T. et al., Wavelength and NOx dependent complex refractive index of SOAs generated from the photooxidation of toluene, Atmos. Chem. Phys., 13, 531-545 (2013). Nakayama, T. et al., Properties of light-absorbing aerosols in the Nagoya urban area, Japan, in August 2011 and January 2012: Contributions of brown carbon and lensing effect, J. Geophys. Res. Atmos., 119, 12721-12739 (2014). Nakayama, T. et al., Characterization of a three wavelength photoacoustic soot spectrometer (PASS-3) and a photoacoustic extinctiometer (PAX), J. Meteor. Soc. Japan, (2015, in press).

  11. Laboratory techniques and rhythmometry

    NASA Technical Reports Server (NTRS)

    Halberg, F.

    1973-01-01

    Some of the procedures used for the analysis of rhythms are illustrated, notably as these apply to current medical and biological practice. For a quantitative approach to medical and broader socio-ecologic goals, the chronobiologist gathers numerical objective reference standards for rhythmic biophysical, biochemical, and behavioral variables. These biological reference standards can be derived by specialized computer analyses of largely self-measured (until eventually automatically recorded) time series (autorhythmometry). Objective numerical values for individual and population parameters of reproductive cycles can be obtained concomitantly with characteristics of about-yearly (circannual), about-daily (circadian) and other rhythms.

  12. Advanced x-ray spectrometric techniques for characterization of nuclear materials: An overview of recent laboratory activities

    NASA Astrophysics Data System (ADS)

    Misra, N. L.

    2014-11-01

    Advancements in x-ray spectrometric techniques at different stages have made this technique suitable for characterization of nuclear materials with respect to trace/major element determinations and compositional uniformity studies. The two important features of total reflection x-ray fluorescence spectrometry: 1) requirement of very small amount of sample in ng level 2) multielement analytical capability, in addition to other features, make this technique very much suitable to nuclear materials characterization as most of the nuclear materials are radioactive and the radioactive waste generated and radiation hazards to the operator are minimum when such low amount of sample is used. Similarly advanced features of energy dispersive x-ray fluorescence e.g. better geometry for high flux, reduction in background due to application of radiation filters have made the measurements of samples sealed inside thin alkathene/PVC covers possible with good sensitivity. This approach avoids putting the instrument inside a glove box for measuring radioactive samples and makes the operation/maintenance of the instrument and analysis of the samples possible in easy and fast manner. This approach has been used for major element determinations in mixed uranium-plutonium samples. Similarly μ-XRF with brilliant and micro-focused excitation sources can be used for compositional uniformity study of reactor fuel pellets. A μ-XRF study using synchrotron light source has been made to assess the compositional uniformity of mixed uranium-thorium oxide pellets produced by different processes. This approach is simple as it does not involve any sample preparation and is non-destructive. A brief summary of such activities carried out in our laboratory in past as well as ongoing and planned for the future have been discussed in the present manuscript.

  13. Effects of Rearing Density on Survival, Growth, and Development of the Ladybird Coleomegilla maculata in Culture

    PubMed Central

    Riddick, Eric W.; Wu, Zhixin

    2015-01-01

    Our research focuses on developing techniques to rear ladybird beetles (Coleoptera: Coccinellidae). We evaluated the effects of rearing density on survival, growth, and development of Coleomegilla maculata. The hypothesis that a low to moderate rearing density has limited or no effects on survival and development was tested. C. maculata first instars were reared to pupae at a density of 1, 5, 10, 15, or 20 individuals per arena (2.5 cm high, 9.0 cm diameter, and 159 cm3 volume) and fed powdered brine shrimp (Artemia franciscana) eggs. More larvae survived at the 1 and 5 densities, but no differences were detected between the 10, 15, or 20 densities. Median survival rate was at least 90% for larvae and 100% for pupae at the 10, 15, and 20 densities. Development time, body weight, and sex ratio were unaffected by rearing density. Overall, this study suggests that C. maculata larvae can be reared successfully at a density of 20 larvae/159 cm3 (≈ 0.126 larvae/cm3) in containers provisioned with powdered A. franciscana eggs. Scaling-up the size of containers, and C. maculata density in these containers, should be possible. PMID:26466904

  14. Persistence of free-living protozoan communities across rearing cycles in commercial poultry houses.

    PubMed

    Baré, Julie; Houf, Kurt; Verstraete, Tine; Vaerewijck, Mario; Sabbe, Koen

    2011-03-01

    The introduction and survival of zoonotic bacterial pathogens in poultry farming have been linked to bacterial association with free-living protozoa. To date, however, no information is available on the persistence of protozoan communities in these environments across consecutive rearing cycles and how it is affected by farm- and habitat-specific characteristics and management strategies. We therefore investigated the spatial and temporal dynamics of free-living protozoa in three habitats (pipeline, water, and miscellaneous samples) in three commercial poultry houses across three rearing cycles by using the molecular fingerprinting technique denaturing gradient gel electrophoresis (DGGE). Our study provides strong evidence for the long-term (ca. 6-month) persistence of protozoa in broiler houses across consecutive rearing cycles. Various free-living protozoa (flagellates, ciliates, and amoebae), including known vectors of bacterial pathogens, were observed during the down periods in between rearing cycles. In addition, multivariate analysis and variation partitioning showed that the protozoan community structure in the broiler houses showed almost no change across rearing cycles and remained highly habitat and farm specific. Unlike in natural environments, protozoan communities inside broiler houses are therefore not seasonal. Our results imply that currently used biosecurity measures (cleaning and disinfection) applied during the down periods are not effective against many protozoans and therefore cannot prevent potential cross-contamination of bacterial pathogens via free-living protozoa between rearing cycles. PMID:21239551

  15. Effects of Rearing Density on Survival, Growth, and Development of the Ladybird Coleomegilla maculata in Culture.

    PubMed

    Riddick, Eric W; Wu, Zhixin

    2015-01-01

    Our research focuses on developing techniques to rear ladybird beetles (Coleoptera: Coccinellidae). We evaluated the effects of rearing density on survival, growth, and development of Coleomegilla maculata. The hypothesis that a low to moderate rearing density has limited or no effects on survival and development was tested. C. maculata first instars were reared to pupae at a density of 1, 5, 10, 15, or 20 individuals per arena (2.5 cm high, 9.0 cm diameter, and 159 cm3 volume) and fed powdered brine shrimp (Artemia franciscana) eggs. More larvae survived at the 1 and 5 densities, but no differences were detected between the 10, 15, or 20 densities. Median survival rate was at least 90% for larvae and 100% for pupae at the 10, 15, and 20 densities. Development time, body weight, and sex ratio were unaffected by rearing density. Overall, this study suggests that C. maculata larvae can be reared successfully at a density of 20 larvae/159 cm3 (≈ 0.126 larvae/cm3) in containers provisioned with powdered A. franciscana eggs. Scaling-up the size of containers, and C. maculata density in these containers, should be possible. PMID:26466904

  16. Physiological consequences of laboratory rearing of Lygus hesperus (Hemiptera: Miridae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Several aspects of the basic biology of the western tarnished plant bug, Lygus hesperus Knight, are poorly known despite of the economic importance of this species. Among these are the factors regulating the adult diapause. Recent studies questioned the validity of earlier reports of diapause in L...

  17. GENETIC PARAMETERS AND EVALUATION OF REAR LEGS (REAR VIEW) FOR BROWN SWISS AND GUERNSEYS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Genetic parameters for rear legs/rear view (RLRV) and 15 current linear type traits in Brown Swiss (BS) and Guernsey (GU) dairy cattle were estimated. The BS Association began scoring RLRV in 2004 and the American GU Association began in 2002. For BS, 7,522 records were available on 6,896 cows in 37...

  18. In vitro rearing of Perkinsiella saccharicida and the use of leaf segments to assay Fiji disease virus transmission.

    PubMed

    Hughes, G L; Allsopp, P G; Brumbley, S M; Johnson, K N; O'Neill, S L

    2008-07-01

    Fiji leaf gall (FLG) is caused by the Reovirus, Fiji disease virus (FDV), which is transmitted to sugarcane by planthoppers of the genus Perkinsiella. Low vector transmission rates and slow disease symptom development make experimentation within the FDV-Perkinsiella-sugarcane system inherently difficult. A laboratory-based technique was devised to rear the vector using sugarcane leaves as a food source. Planthoppers were reared on sugarcane leaf segments embedded in agarose enclosed within plastic containers. To provide a nondestructive assay for determination of the inoculation potential of planthoppers, FDV was detected by reverse transcription-polymerase chain reaction (RT-PCR) in newly infected sugarcane leaf segments following exposure to viruliferous planthoppers. Leaf segment inoculation correlated with development of FLG symptoms in whole plants that were fed on by the same planthoppers. Analysis of FDV RNAs within the planthopper, measured by quantitative RT-PCR (qRT-PCR), indicated that FDV RNA concentration was associated with successful inoculation of the leaf segment, transmission of FDV to sugarcane and subsequent development of FLG in plants. Quantification of FDV RNA within planthoppers provided an additional measure to assess vector competence in individuals. PMID:18943257

  19. Composition of the Spruce Budworm (Choristoneura fumiferana) Midgut Microbiota as Affected by Rearing Conditions

    PubMed Central

    Landry, Mathieu; Comeau, André M.; Derome, Nicolas; Cusson, Michel; Levesque, Roger C.

    2015-01-01

    The eastern spruce budworm (Choristoneura fumiferana) is one of the most destructive forest insect pests in Canada. Little is known about its intestinal microbiota, which could play a role in digestion, immune protection, communication and/or development. The present study was designed to provide a first characterization of the effects of rearing conditions on the taxonomic diversity and structure of the C. fumiferana midgut microbiota, using a culture-independent approach. Three diets and insect sources were examined: larvae from a laboratory colony reared on a synthetic diet and field-collected larvae reared on balsam fir or black spruce foliage. Bacterial DNA from the larval midguts was extracted to amplify and sequence the V6-V8 region of the 16S rRNA gene, using the Roche 454 GS-FLX technology. Our results showed a dominance of Proteobacteria, mainly Pseudomonas spp., in the spruce budworm midgut, irrespective of treatment group. Taxonomic diversity of the midgut microbiota was greater for larvae reared on synthetic diet than for those collected and reared on host plants, a difference that is likely accounted for by several factors. A greater proportion of bacteria from the phylum Bacteroidetes in insects fed artificial diet constituted the main difference between this group and those reared on foliage; within the phylum Proteobacteria, the presence of the genus Bradyrhizobium was also unique to insects reared on artificial diet. Strikingly, a Bray-Curtis analysis showed important differences in microbial diversity among the treatment groups, pointing to the importance of diet and environment in defining the spruce budworm midgut microbiota. PMID:26636571

  20. Hand-Rearing Reduces Fear of Humans in European Starlings, Sturnus vulgaris

    PubMed Central

    Feenders, Gesa; Bateson, Melissa

    2011-01-01

    Pending changes in European legislation ban the use of wild-caught animals in research. This change is partly justified on the assumption that captive-breeding (or hand-rearing) increases welfare of captive animals because these practices result in animals with reduced fear of humans. However, there are few actual data on the long-term behavioural effects of captive-breeding in non-domestic species, and these are urgently needed in order to understand the welfare and scientific consequences of adopting this practice. We compared the response of hand-reared and wild-caught starlings to the presence of a human in the laboratory. During human presence, all birds increased their general locomotor activity but the wild-caught birds moved away from the human and were less active than the hand-reared birds. After the human departed, the wild-caught birds were slower to decrease their activity back towards baseline levels, and showed a dramatic increase in time at the periphery of the cage compared with the hand-reared birds. We interpret these data as showing evidence of a greater fear response in wild-caught birds with initial withdrawal followed by a subsequent rebound of prolonged attempts to escape the cage. We found no effects of environmental enrichment. However, birds in cages on low shelves were less active than birds on upper shelves, and showed a greater increase in the time spent at the periphery of their cages after the human departed, perhaps indicating that the lower cages were more stressful. In demonstrating reduced fear of humans in hand-reared birds, our results support one of the proposed welfare benefits of this practice, but without further data on the possible welfare costs of hand-rearing, it is not yet possible to reach a general conclusion about its net welfare impact. However, our results confirm a clear scientific impact of both hand-rearing and cage position at the behavioural level. PMID:21364770

  1. Growth and social behavior in a cichlid fish are affected by social rearing environment and kinship

    NASA Astrophysics Data System (ADS)

    Hesse, Saskia; Thünken, Timo

    2014-04-01

    Living in groups is a widespread phenomenon in many animal taxa. The reduction of predation risk is thought to be an important cause for the formation of groups. Consequently, grouping behavior is particularly pronounced during vulnerable life stages, i.e., as juveniles. However, group living does not only provide benefits but also imposes costs on group members, e.g., increased competition for food. Thus, benefits of grouping behavior might not be evident when predation risk is absent. The adaptive significance of living and also developing in a group independent from predation risk has received relatively little attention although this might have important implications on the evolution and maintenance of group living. The first aim of the present study was to examine whether the social environment affects juvenile performance in the cichlid fish Pelvicachromis taeniatus and, secondly, whether kinship affects social behavior. Kin selection theory predicts benefits from grouping with kin. Here, we demonstrate that juveniles reared in a group grow on average faster compared to juveniles reared in isolation under standardized laboratory conditions without predation risk. Furthermore, we found significant differences in social behavior between juveniles reared in a group and reared in isolation. Fish reared in isolation were significantly more aggressive and less willing to shoal than group-reared fish. As expected, genetic relatedness influenced social behavior in group-reared fish as well: dyads of juveniles consisting of kin showed increased group cohesiveness compared to non-kin dyads. We discuss the potential benefits of group living in general and living with kin in particular.

  2. Evaluating High-Throughput Ab Initio Gene Finders to Discover Proteins Encoded in Eukaryotic Pathogen Genomes Missed by Laboratory Techniques

    PubMed Central

    Goodswen, Stephen J.; Kennedy, Paul J.; Ellis, John T.

    2012-01-01

    Next generation sequencing technology is advancing genome sequencing at an unprecedented level. By unravelling the code within a pathogen’s genome, every possible protein (prior to post-translational modifications) can theoretically be discovered, irrespective of life cycle stages and environmental stimuli. Now more than ever there is a great need for high-throughput ab initio gene finding. Ab initio gene finders use statistical models to predict genes and their exon-intron structures from the genome sequence alone. This paper evaluates whether existing ab initio gene finders can effectively predict genes to deduce proteins that have presently missed capture by laboratory techniques. An aim here is to identify possible patterns of prediction inaccuracies for gene finders as a whole irrespective of the target pathogen. All currently available ab initio gene finders are considered in the evaluation but only four fulfil high-throughput capability: AUGUSTUS, GeneMark_hmm, GlimmerHMM, and SNAP. These gene finders require training data specific to a target pathogen and consequently the evaluation results are inextricably linked to the availability and quality of the data. The pathogen, Toxoplasma gondii, is used to illustrate the evaluation methods. The results support current opinion that predicted exons by ab initio gene finders are inaccurate in the absence of experimental evidence. However, the results reveal some patterns of inaccuracy that are common to all gene finders and these inaccuracies may provide a focus area for future gene finder developers. PMID:23226328

  3. Evaluating high-throughput ab initio gene finders to discover proteins encoded in eukaryotic pathogen genomes missed by laboratory techniques.

    PubMed

    Goodswen, Stephen J; Kennedy, Paul J; Ellis, John T

    2012-01-01

    Next generation sequencing technology is advancing genome sequencing at an unprecedented level. By unravelling the code within a pathogen's genome, every possible protein (prior to post-translational modifications) can theoretically be discovered, irrespective of life cycle stages and environmental stimuli. Now more than ever there is a great need for high-throughput ab initio gene finding. Ab initio gene finders use statistical models to predict genes and their exon-intron structures from the genome sequence alone. This paper evaluates whether existing ab initio gene finders can effectively predict genes to deduce proteins that have presently missed capture by laboratory techniques. An aim here is to identify possible patterns of prediction inaccuracies for gene finders as a whole irrespective of the target pathogen. All currently available ab initio gene finders are considered in the evaluation but only four fulfil high-throughput capability: AUGUSTUS, GeneMark_hmm, GlimmerHMM, and SNAP. These gene finders require training data specific to a target pathogen and consequently the evaluation results are inextricably linked to the availability and quality of the data. The pathogen, Toxoplasma gondii, is used to illustrate the evaluation methods. The results support current opinion that predicted exons by ab initio gene finders are inaccurate in the absence of experimental evidence. However, the results reveal some patterns of inaccuracy that are common to all gene finders and these inaccuracies may provide a focus area for future gene finder developers. PMID:23226328

  4. Interaction of rearing environment and reproductive tactic on gene expression profiles in Atlantic salmon

    USGS Publications Warehouse

    Aubin-Horth, N.; Letcher, B.H.; Hofmann, H.A.

    2005-01-01

    Organisms that share the same genotype can develop into divergent phenotypes, depending on environmental conditions. In Atlantic salmon, young males of the same age can be found either as sneakers or immature males that are future anadromous fish. Just as the organism-level phenotype varies between divergent male developmental trajectories, brain gene expression is expected to vary as well. We hypothesized that rearing environment can also have an important effect on gene expression in the brain and possibly interact with the reproductive tactic adopted. We tested this hypothesis by comparing brain gene expression profiles of the two male tactics in fish from the same population that were reared in either a natural stream or under laboratory conditions. We found that expression of certain genes was affected by rearing environment only, while others varied between male reproductive tactics independent of rearing environment. Finally, more than half of all genes that showed variable expression varied between the two male tactics only in one environment. Thus, in these fish, very different molecular pathways can give rise to similar macro-phenotypes depending on rearing environment. This result gives important insights into the molecular underpinnings of developmental plasticity in relationship to the environment. ?? 2005 The American Genetic Association.

  5. The Role of Socio-Communicative Rearing Environments on the Development of Social and Physical Cognition in Apes

    PubMed Central

    Russell, J. L.; Lyn, H.; Schaeffer, J. A.; Hopkins, W. D.

    2011-01-01

    The cultural intelligence hypothesis (CIH) claims that humans' advanced cognition is a direct result of human culture and that children are uniquely specialized to absorb and utilize this cultural experience (Tomasello, 2000). Comparative data demonstrating that 2.5 year old human children outperform apes on measures of social cognition but not on measures of physical cognition support this claim (E. Herrmann, J. Call, M. V. Hernandez-Lloreda, B. Hare, & M. Tomasello, 2007). However, the previous study failed to control for rearing when comparing these two species. Specifically, the human children were raised in a human culture whereas the apes were raised in standard sanctuary settings. To further explore the CIH, here we compared the performance on multiple measures of social and physical cognition in a group of standard reared apes raised in conditions typical of zoo and biomedical laboratory settings to that of apes reared in an enculturated socio-communicatively rich environment. Overall, the enculturated apes significantly outperformed their standard reared counterparts on the cognitive tasks and this was particularly true for measures of communication. Furthermore, the performance of the enculturated apes was very similar to previously reported data from 2.5 year old children. We conclude that apes who are reared in a human-like socio-communicatively rich environment develop superior communicative abilities compared to apes reared in standard laboratory settings, which supports some assumptions of the cultural intelligence hypothesis. PMID:22010903

  6. Mass rearing of Lucilia sericata Meigen (Diptera: Calliphoridae)

    PubMed Central

    Firoozfar, F; Moosa-Kazemi, H; Baniardalani, M; Abolhassani, M; Khoobdel, M; Rafinejd, J

    2011-01-01

    Objective To carry out an experimental study with the main objective of mass rearing of sheep flies (Lucilia sericata). Methods Hand collection and beef- or cattle liver-baited net traps were used for field fly sampling from April, 2010 to November, 2010. The samples collected from different places were placed in properly labeled tubes and sent to the Entomology Laboratory. Since maggot identification is important in inducing mortality, they were kept under insectary condition to develop to adult stage and identified using systematic keys. Results A total of 218 flies were collected in three rounds of sampling from the field of Tehran and Karaj Counties. In the first generation, 433 flies including 135 (31.17%) male, and 298 (68.82%) female were yielded. The female/male of parent ratio was calculated as 1.72 in Tehran and in Karaj areas, whereas it was 2.20% and 1.81%, respectively in F1 and F2 generations, respectively. Conclusions During this study, the mass rearing of sheep blow fly has been established at the School of Public Health, Tehran University of Medical Sciences and can be used for producing flies for maggot therapy. PMID:23569725

  7. Child Rearing Study in Brunei Darussalam.

    ERIC Educational Resources Information Center

    Rosberg, Marilee A.

    In order to gather data on children's lives, language, and religious activities, and to gather data on child rearing practices in Brunei, a study interviewed parents from 38 Malaysian families having one or more children 3-8 years old. Results indicated that 92 percent of the children crawled when they were between 6-9 months old; 63 percent were…

  8. BOILING HOUSE, GROUND FLOOR. WAREHOUSE TO LEFT REAR, MASSECUITTE HEATERS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    BOILING HOUSE, GROUND FLOOR. WAREHOUSE TO LEFT REAR, MASSECUITTE HEATERS ABOVE RIGHT, LOW GRADE CENTRIFUGALS BELOW. CRYSTALLIZER HOT WATER TANK TO REAR. VIEW FROM NORTHEAST - Lihue Plantation Company, Sugar Mill Building, Haleko Road, Lihue, Kauai County, HI

  9. EVAPORATOR FLOOR, CELL ONE IN FOREGROUND, CRYSTALLIZER MEZZANINE TO REAR, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    EVAPORATOR FLOOR, CELL ONE IN FOREGROUND, CRYSTALLIZER MEZZANINE TO REAR, OLIVER MUD FILTER WING TO RIGHT REAR. VIEW FROM NORTHEAST - Lihue Plantation Company, Sugar Mill Building, Haleko Road, Lihue, Kauai County, HI

  10. 53. REAR OF MOTOR AND REDUCTION GEAR NO. 2: View ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    53. REAR OF MOTOR AND REDUCTION GEAR NO. 2: View towards northwest showing rear of Motor and Reduction Gear No. 2, installed in 1926. - San Francisco Cable Railway, Washington & Mason Streets, San Francisco, San Francisco County, CA

  11. 31. REAR OF CAR BARN DURING RECONSTRUCTION: Photocopy of July ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    31. REAR OF CAR BARN DURING RECONSTRUCTION: Photocopy of July 1908 photograph showing west rear of powerhouse and car barn. View from the north. - San Francisco Cable Railway, Washington & Mason Streets, San Francisco, San Francisco County, CA

  12. TRACES OF ORIGINAL PARTITIONS AT JUNCTURE OF FRONT ROOM, REAR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    TRACES OF ORIGINAL PARTITIONS AT JUNCTURE OF FRONT ROOM, REAR ROOM AND HALL, SECOND FLOOR. ALSO SHOWS ORIGINAL STUCCO CORNICE OF FRONT AND REAR ROOMS (LEFT) AND HALL (RIGHT) - Kid-Chandler House, 323 Walnut Street, Philadelphia, Philadelphia County, PA

  13. Eye oscillations in strobe reared cats.

    PubMed

    Melvill Jones, G; Mandl, G; Cynader, M; Outerbridge, J S

    1981-03-23

    Cats reared from birth in stroboscopic illumination develop abnormal spontaneous eye oscillations of low amplitude. The present experiments were undertaken to define these eye movements as recorded in the dark, in stroboscopic light of various frequencies, after exposure to normal light and after attenuation of the vestibulo-ocular reflex (VOR) gain by optical reversal of vision. The interaction of spontaneous eye oscillations with voluntary saccadic eye movements, and optokinetic tracking (OKN), were also studied. Two cats, reared from birth to 18 months in 8 Hz strobe light, and one normally reared control animal, were used. Horizontal movement of the right eye was measured by the scleral eye coil method. The frequency content of eye movement records was determined by power spectral analysis. VOR gain was estimated in the dark, by rotating the animals sinusoidally at 1/8 Hz and 5 degrees/sec velocity amplitude. In the dark, both strobe reared cats had abnormal spontaneous eye oscillations at a frequency close to 8 Hz, with peak-to-peak amplitudes of 0.5--1.0 degrees. These abnormal eye movements did not interfere with, nor were they abolished by, normal oculomotor activity. The introduction of strobe light modified the spontaneous eye movements by entraining the oscillations at a given 'forcing' frequency, and by producing a number of harmonics or sub-harmonics. In one of the strobe reared animals, the effect of normal light was to reduce the characteristic 'dark' value of 9 Hz, to a new maintained 'light' value of 2.7 Hz. Adaptive attenuation of the VOR gain caused the abolition of regular spontaneous eye oscillations in the dark; nevertheless, transient oscillations to single strobe flashes could still be elicited in the VOR adapted condition. The results are interpreted as representing an organised attempt by the developing oculomotor system to attain the goal of stable visual perception in a new visual environment. PMID:7214163

  14. Identification of volatiles from waste larval rearing media that attract gravid screwworm flies to oviposit

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The waste product of the artificial larval rearing media of the primary screwworm, Cochliomyia hominivorax, attracts gravid female screwworm flies to oviposit. The volatile component of this waste product was collected using solid phase microextraction techniques and subjected to gas chromatography-...

  15. NOVEL ASPECTS OF SPOTTED WING DROSOPHILA BIOLOGY AND IMPROVED METHODS OF REARING

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Drosophila suzukii (Mats.) or the spotted wing Drosophila (SWD), is a global pest of soft fruits that can now be reared on a standard Drosophila diet containing the fly's own natural food: soft-skinned berries. The techniques tested here can thwart bacterial and fungal disease that can destroy more ...

  16. Inbreeding Effects in Families of Ostrinia nubilalis (Lepidoptera: Crambidae): Larval Development in Laboratory Bioassays

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Inbreeding depression of laboratory-reared insects has the potential to affect their larval performance and reproductive output. Two studies of laboratory-reared colonies of Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae) were conducted to assess whether inbreeding affected a laboratory bioass...

  17. Combining impact sensor field and laboratory flume measurements with other techniques for studying fluvial bedload transport in steep mountain streams

    NASA Astrophysics Data System (ADS)

    Beylich, Achim A.; Laute, Katja

    2014-08-01

    The timing and rate of fluvial bedload transport are of central importance within sediment budget studies and in many applications in river science and engineering. During the years 2010, 2011 and 2012 detailed field measurements with portable impact sensors as a non-invasive technique for indirectly determining fluvial bedload transport intensity were conducted in two instrumented and supply-limited drainage basin systems (Erdalen and Bødalen) in the fjord landscape in western Norway. Additional field measurements with portable impact sensors were carried out in 2010 and 2011 in selected transport-limited fluvial systems in the Coast Mountains of western Canada. The collected impact sensor field data were calibrated with laboratory flume experiments. The data from the impact sensor field measurements in western Norway and the flume experiments were combined with field data from continuous discharge monitoring, repeated surveys of channel morphometry and sediment texture, particle tracer measurements, Helley-Smith samplings, underwater video filming and biofilm analyses. The combination of methods and techniques applied provides insights into the temporal variability and intensity of fluvial bedload transport in the selected mountain streams: (i) in the transport-limited systems with generally high bedload transport rates during high discharge and with bedload material moving in clusters over the impact sensor plates, impact sensor data (based on a 1 s measuring interval) provide the opportunity to detect the start and end of bedload transport, thus to identify discharge thresholds for sediment entrainment, and to roughly estimate the intensity and relative intensity of change of bedload transport during the measuring period; (ii) in the supply-limited systems with low bedload transport rates and bedload components moving separately (as single particles) over the impact sensor plates, impact sensor data (with a 1 s measuring interval) allow the detection of the start and end of transport of bedload components > 11.3 mm, thus the identification of discharge thresholds for possible entrainment of particles, the quantification of the number of particles > 11.3 mm moving over the impact sensor plates during the measuring period, the rough estimation of grain sizes of the particles moving separately over the impact sensor plates, and the calculation of the total mass of the bedload material > 11.3 mm moving over the impact sensor plates during the measuring period; (iii) when combined with other methods and techniques (Helley-Smith sampling, particle tracer measurements, biofilm analyses, underwater video filming) which provide information on the active bedload transport channel width, on discharge thresholds for possible entrainment of particles of different grain sizes, and on transport rates of bedload material < 11.3 mm, total rates of fluvial bedload transport, covering all given grain sizes of the bedload material, can be calculated for the supply-limited mountain streams with generally low bedload transport. The higher measured annual bedload yield in Bødalen (13.6 t km- 2 yr- 1) compared to Erdalen (2.6 t km- 2 yr- 1) reflects a higher level of slope-channel coupling in Bødalen than in Erdalen.

  18. 6. Partial view of rear elevations of shops building and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Partial view of rear elevations of shops building and Hangar 1301 with rear elevation of corridor (behind power plant) connecting the hangar and shops building. Side elevations of shops and hangar as well as upper rear story of power plant and stack are visible, looking south southwest - Dover Air Force Base, Hangar No. 1301, Dover, Kent County, DE

  19. 24. REAR ELEVATION, HULETT ORE UNLOADERS. TRACKS CARRYING THE FRONT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    24. REAR ELEVATION, HULETT ORE UNLOADERS. TRACKS CARRYING THE FRONT END AND REAR LEGS OF THE HULETT UNLOADERS ARE LAID ON THE DOCK AND REAR WALLS, RESPECTIVELY; BOTH WALLS ARE MADE OF REINFORCED CONCRETE SUPPORTED ON CONCRETE PILES. - Pennsylvania Railway Ore Dock, Lake Erie at Whiskey Island, approximately 1.5 miles west of Public Square, Cleveland, Cuyahoga County, OH

  20. GARAGE EXTERIOR EAST SIDE AND REAR SHOWING PIER SUPPORTS UNDER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    GARAGE EXTERIOR EAST SIDE AND REAR SHOWING PIER SUPPORTS UNDER SHED-ROOFED REAR STORAGE COMPARTMENT, ASBESTOS SIDING OVER ORIGINAL WOOD SIDING, AND SINGLE CASEMENT WINDOW OVER REAR STORAGE COMPARTMENT. VIEW TO NORTHWEST - Big Creek Hydroelectric System, Big Creek Town, Operator House Garage, Orchard Avenue south of Huntington Lake Road, Big Creek, Fresno County, CA

  1. 13. Dairy barn, west side, rear stall wing to left, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Dairy barn, west side, rear stall wing to left, rear yard at center, and rear yard wall to right - A. I. Du Pont Estate, Blue Ball Dairy Barn, Junction of U.S. Route 202 & Rockland Road, Wilmington, New Castle County, DE

  2. A comparison of haemolytic responses in fore-foot and rear-foot distance runners.

    PubMed

    Caulfield, Stuart; McDonald, Kirsty A; Dawson, Brian; Stearne, Sarah M; Green, Ben A; Rubenson, Jonas; Clemons, Tristan D; Peeling, Peter

    2016-08-01

    This study examined the haemolytic effects of an interval-based running task in fore-foot and rear-foot striking runners. Nineteen male distance runners (10 fore-foot, 9 rear-foot) completed 8 × 3 min repeats at 90% vVO2peak on a motorised treadmill. Pre- and post-exercise venous blood samples were analysed for serum haptoglobin to quantify the haemolytic response to running. Vertical ground reaction forces were also captured via a force plate beneath the treadmill belt. Haptoglobin levels were significantly decreased following exercise (P = 0.001) in both groups (but not between groups), suggesting that the running task created a haemolytic stress. The ground reaction force data showed strong effect sizes for a greater peak force (d = 1.20) and impulse (d = 1.37) in fore-foot runners, and a greater rate of force development (d = 2.74) in rear-foot runners. The lack of difference in haptoglobin response between groups may be explained by the trend for fore-foot runners to experience greater peak force and impulse during the stance phase of their running gait, potentially negating any impact of the greater rate of force development occurring from the rear-foot runners' heel strike. Neither type of runner (fore-foot or rear-foot) appears more susceptible to technique-related foot-strike haemolysis. PMID:26618486

  3. Basic biology and small-scale rearing of Celatoria compressa(Diptera: Tachinidae), a parasitoid of Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae).

    PubMed

    Zhang, F; Toepfer, S; Kuhlmann, U

    2003-12-01

    The tachinid Celatoria compressa Wulp has been evaluated as a candidate biological control agent for the western corn rootworm, Diabrotica virgifera virgiferaLeConte, in Europe, where it is an invasive alien pest of maize. Special emphasis has been placed on understanding aspects of the parasitoid basic biology and on developing a rearing technique for a small-scale production of C. compressapuparia. The age of C. compressa adults was found to be the most crucial factor in achieving mating. Only newly emerged, 1-h-old females, mated successfully with 2- to 5-day-old males, achieving a success rate of 74%. After mating, a prelarviposition period of 4 days occurred. The 5-day-old C. compressa females inserted their eggs containing fully-developed first instars directly into adults of D. v. virgifera. Total larval and pupal developmental time, including a pre-larviposition period of 4 days, was 29 days under quarantine laboratory conditions (25 degrees C daytime, 15 degrees C at night, L:D 14:10, 50% +/- 10% r.h). Females of C. compressa were capable of producing on average 30 puparia throughout a female's mean larviposition period of 15 days. A large number of host attacks by C. compressa were unsuccessful, resulting in a mean larviposition success rate of 24% per female. Parasitoid females appear to have difficulties inserting the egg through the intersegmental sutures or membranes around leg openings of the host adults. Although the small-scale rearing technique of C. compressa presented is both time and labour intensive, C. compressa has been reared successfully for at least 20 successive generations without shifting the 1 male : 1 female sex ratio using a non-diapause strain of D. v. virgifera. PMID:14704104

  4. Jamaican child-rearing practices: the role of corporal punishment.

    PubMed

    Smith, Delores E; Mosby, Gail

    2003-01-01

    The family is the most prominent social group that exists. It prepares its members for the various roles they will perform in society. Yet, the literature has unequivocally singled out the family as the most violent social group, with parental violence against children being the most prevalent type of family violence. While societies like the United States, Japan, and Sweden have taken a hard line on physical punishment and shifted to a gentler approach to discipline, harsh disciplining of children persists elsewhere. In the Caribbean, and Jamaica in particular, child-rearing and disciplinary practices that would warrant child abuse charges in other Western societies are rampant. This article examines the child-rearing techniques of Jamaican adults and their assumed effects on child outcomes. It also examines the plausibility of the assumption that the harsh physical punishment meted out to children is partially responsible for the current social problems of that island nation. We recommend approaches to tackle the broad goals of addressing familial and societal practices that compromise children's development and well-being. PMID:14560888

  5. Experimental Studies of Surface-Driven Capillary Flow in PMMA Microfluidic Devices Prepared by Direct Bonding Technique and Passive Separation of Microparticles in Microfluidic Laboratory-On Systems

    NASA Astrophysics Data System (ADS)

    Mukhopadhyay, Subhadeep; Banerjee, J. P.; Mathur, Ashish; Tweedie, M.; McLaughlin, J. A.; Roy, Susanta Sinha

    2015-05-01

    Proper bonding technique is investigated to achieve leakage-free surface-driven capillary flow in polymethylmethacrylate (PMMA) microfluidic devices. SU-8-based silicon stamp is fabricated by maskless lithography. This stamp is used to produce PMMA microchannel structure by hot embossing lithography. A direct bonding technique is mainly employed for leakage-free sealing inside PMMA microfluidic devices. The effect of surface wettability on surface-driven capillary flow is also investigated in PMMA microfluidic devices. The separation of polystyrene microparticles in PMMA laboratory-on-a-chip systems is investigated with the reduction of separation time by air dielectric barrier discharge (DBD) plasma processing of channel surfaces. This study is useful to fabricate the microfluidic laboratory-on-a-chip systems and to understand the surface-driven capillary flow.

  6. 10th Anniversary review: applications of analytical techniques in laboratory studies of the chemical and climatic impacts of mineral dust aerosol in the Earth's atmosphere.

    PubMed

    Hatch, Courtney D; Grassian, Vicki H

    2008-08-01

    It is clear that mineral dust particles can impact a number of global processes including the Earth's climate through direct and indirect climate forcing, the chemical composition of the atmosphere through heterogeneous reactions, and the biogeochemistry of the oceans through dust deposition. Thus, mineral dust aerosol links land, air, and oceans in unique ways unlike any other type of atmospheric aerosol. Quantitative knowledge of how mineral dust aerosol impacts the Earth's climate, the chemical balance of the atmosphere, and the biogeochemistry of the oceans will provide a better understanding of these links and connections and the overall impact on the Earth system. Advances in the applications of analytical laboratory techniques have been critical for providing valuable information regarding these global processes. In this mini review article, we discuss examples of current and emerging techniques used in laboratory studies of mineral dust chemistry and climate and potential future directions. PMID:18688461

  7. Morphometric Identification of Queens, Workers and Intermediates in In Vitro Reared Honey Bees (Apis mellifera)

    PubMed Central

    A. De Souza, Daiana; Wang, Ying; Kaftanoglu, Osman; De Jong, David; V. Amdam, Gro; S. Gonçalves, Lionel; M. Francoy, Tiago

    2015-01-01

    In vitro rearing is an important and useful tool for honey bee (Apis mellifera L.) studies. However, it often results in intercastes between queens and workers, which are normally are not seen in hive-reared bees, except when larvae older than three days are grafted for queen rearing. Morphological classification (queen versus worker or intercastes) of bees produced by this method can be subjective and generally depends on size differences. Here, we propose an alternative method for caste classification of female honey bees reared in vitro, based on weight at emergence, ovariole number, spermatheca size and size and shape, and features of the head, mandible and basitarsus. Morphological measurements were made with both traditional morphometric and geometric morphometrics techniques. The classifications were performed by principal component analysis, using naturally developed queens and workers as controls. First, the analysis included all the characters. Subsequently, a new analysis was made without the information about ovariole number and spermatheca size. Geometric morphometrics was less dependent on ovariole number and spermatheca information for caste and intercaste identification. This is useful, since acquiring information concerning these reproductive structures requires time-consuming dissection and they are not accessible when abdomens have been removed for molecular assays or in dried specimens. Additionally, geometric morphometrics divided intercastes into more discrete phenotype subsets. We conclude that morphometric geometrics are superior to traditional morphometrics techniques for identification and classification of honey bee castes and intermediates. PMID:25894528

  8. Development of a Natural Rearing System to Improve Supplemental Fish Quality, 1991-1995 Progress Report.

    SciTech Connect

    Maynard, Desmond J.; Flagg, Thomas A.; Mahnken, Conrad V.W.

    1996-08-01

    In this report, the National Marine Fisheries Service (NMFS), in collaboration with the Bonneville Power Administration (BPA), the Washington State Department of Fish and Wildlife (WDFW), and the US Fish and Wildlife Service (USFWS), presents research findings and guidelines for development and evaluation of innovative culture techniques to increase postrelease survival of hatchery fish. The Natural Rearing Enhancement System (NATURES) described in this report is a collection of experimental approaches designed to produce hatchery-reared chinook salmon (Oncorhynchus tshawytscha) that exhibit wild-like behavior, physiology, and morphology. The NATURES culture research for salmonids included multiple tests to develop techniques such as: raceways equipped with cover, structure, and natural substrates to promote development of proper body camouflage coloration; feed-delivery systems that condition fish to orient to the bottom rather than the surface of the rearing vessel; predator conditioning of fish to train them to avoid predators; and supplementing diets with natural live foods to improve foraging ability. The underlying assumptions are that NATURES will: (1) promote the development of natural cryptic coloration and antipredator behavior; (2) increase postrelease foraging efficiency; (3) improve fish health and condition by alleviating chronic, artificial rearing habitat-induced stress; and (4) reduce potential genetic selection pressures induced by the conventional salmon culture environment. A goal in using NATURES is to provide quality fish for rebuilding depleted natural runs.

  9. Lyophilized artificial diet for rearing the Neotropical Euschistus heros (Hemiptera: Pentatomidae).

    PubMed

    Mendoza, Agustín C; da Rocha, Aline C P; Parra, José R P

    2016-01-01

    An artificial diet to mass-rear Euschistus heros (F. 1798) (Hemiptera: Pentatomidae) was developed in the laboratory. Biological studies were conducted under controlled conditions of temperature (25 ± 2°C), RH: 60 ± 10%, and photoperiod of 14:10 (L:D) h. Out of 13 diets tested, 2 diets (D9 and D11) were the most suitable. The artificial diets selected had the same composition (green beans, peanuts, sucrose, water, Nipagin, and sorbic acid) except for different antimicrobial agents (D11 has tetracycline, and D9 doesn't). The 68% viability for the egg-adult period of insects reared on these lyophilized artificial diets (LAD) was almost twice as high as the 38% viability obtained with the natural diet. Although adults reared on LAD weighed 17% less than those reared on the natural diet, mean fecundity was higher than on the natural diet (282 eggs/female), reaching 430 eggs/female. The net reproductive rate (Ro) increased over the generations for the diets with lyophilized material and antimicrobial agents. The opposite occurred with the diet of lyophilized material without antimicrobial agents, showing that the insects either adapted or degenerated through generations. Lyophilized diets supported the production of E. heros through at least 10 generations, with no degeneration. PMID:27126964

  10. Effects of Training Experienced Teachers in the Use of the One-Minute Preceptor Technique in the Gross Anatomy Laboratory

    ERIC Educational Resources Information Center

    Chan, Lap Ki; Sharma, Neel

    2014-01-01

    The one-minute preceptor (OMP) is a time-efficient, learner-centered teaching method used in a busy ambulatory care setting. This project evaluated the effects of training experienced anatomy teachers in the use of the OMP in the gross anatomy laboratory on students' perceived learning. Second-year medical students from a five-year,

  11. Effects of Training Experienced Teachers in the Use of the One-Minute Preceptor Technique in the Gross Anatomy Laboratory

    ERIC Educational Resources Information Center

    Chan, Lap Ki; Sharma, Neel

    2014-01-01

    The one-minute preceptor (OMP) is a time-efficient, learner-centered teaching method used in a busy ambulatory care setting. This project evaluated the effects of training experienced anatomy teachers in the use of the OMP in the gross anatomy laboratory on students' perceived learning. Second-year medical students from a five-year,…

  12. Procedures to mititgate the impact of Solenopsis invicta Virus 3 in fire ant (Hymenoptera:Formicidae) rearing facilities

    Technology Transfer Automated Retrieval System (TEKTRAN)

    During the initial characterization of Solenopsis invicta virus 3 (SINV-3), virus-infected fire ant colonies were retrieved from the field and maintained in the laboratory rearing facility at the USDA, Gainesville, FL. During this time, all S. invicta colonies housed in the facility contracted SINV...

  13. Performance of Psyttalia humilis (Hymenoptera: Braconidae) reared from irradiated host on olive fruit fly (Diptera: Tephritidae) in California

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The parasitoid Psytallia humilis (Silvestri) was reared on Mediterranean fruit fly, Ceratitis capitata (Wiedemann), larvae irradiated at different doses from 0-70 Gy at the USDA, APHIS, PPQ, Moscamed biological control laboratory in San Miguel Petapa, Guatemala and shipped to the USDA, ARS, Parlier,...

  14. Alternative diets for maintaining and rearing cephalopods in captivity.

    PubMed

    DeRusha, R H; Forsythe, J W; DiMarco, F P; Hanlon, R T

    1989-07-01

    The requirement of live marine prey for cephalopod mariculture has restricted its practicality for inland research laboratories, commercial enterprises and home aquarists. We evaluated acceptability and resultant growth on: (a) frozen marine shrimps, (b) live and frozen marine polychaete worms, (c) live and frozen marine crabs, (d) frozen marine fishes, (e) live adult brine shrimp, (f) live freshwater fish and (g) live freshwater crayfish. The diets were presented for periods of 2 to 11 weeks to octopuses, cuttlefishes or squids and in most trials the results were compared to animals fed control diets of live marine shrimps, crabs or fish. Overall, frozen marine shrimp proved to be the best alternative diet tested. Adult Octopus maya on frozen marine shrimp diets grew as well as those on control diets at 2.8% body weight per day (%/d) compared to 2.0%/d on live freshwater crayfish, 1.4%/d on live marine polychaete worms and 0.8%/d on live freshwater fish (Tilapia sp.). Juvenile Octopus maya and Octopus bimaculoides also grew comparably to controls when fed frozen marine shrimps; growth rates ranged from near 3.0%/d at 3 months of age to nearly 2.5%/d at 6 months of age. Thus, these alternatives are acceptable as the octopuses end their exponential growth phase at an age of 3 - 5 months. Attempts to rear O. maya hatchlings and juveniles (up to 1 month of age) on dead foods resulted in high mortality and slow or negative growth. No live or dead alternative diet has been found yet that will promote good growth and survival in hatchling octopuses. Hatchling F3 generation Sepia officinalis (the European cuttlefish) were reared for 6 weeks exclusively on adult brine shrimp (Artemia salina).(ABSTRACT TRUNCATED AT 250 WORDS) PMID:2761235

  15. Diel behavior of rearing fall Chinook salmon

    USGS Publications Warehouse

    Tiffan, Kenneth F.; Kock, Tobias J.; Skalicky, Joseph J.

    2010-01-01

    In fisheries science, habitat use is often inferred when fish are sampled or observed in a particular location. Physical habitat is typically measured where fish are found, and thus deemed important to habitat use. Although less common, a more informative approach is to measure or observe fish behavior within given habitats to more thoroughly assess their use of those locations. While this approach better reflects how fish use habitat, fish behavior can be difficult to quantify, particularly at night. For example, Tiffan and others (2002, 2006) were able to quantify habitat availability and characteristics that were important for rearing juvenile fall Chinook Salmon (Oncorhynchus tshawytscha) in the Hanford Reach of the Columbia River. The authors, however, could only speculate as to how juvenile salmon use habitat and respond to changes in water level fluctuations. Conversely, in this study we provide data on the diel activities of rearing juvenile wild fall Chinook Salmon which provides a better understanding of how fish “use” these rearing habitats. Diel behavior patterns are important because fish in the Hanford Reach are often stranded on shorelines when the water level rapidly recedes because of hydroelectric power generation at upriver dams (Nugent and others 2002; Anglin and others 2006). We hypothesize that juvenile salmon are at greater risk of stranding at night because they are less active and occupy habitat differently than during the day. We used underwater videography to collect behavioral information during the day and night to determine if juvenile fall Chinook Salmon are more susceptible to stranding when water level fluctuations occur at night.

  16. Rear surface effects in high efficiency silicon solar cells

    SciTech Connect

    Wenham, S.R.; Robinson, S.J.; Dai, X.; Zhao, J.; Wang, A.; Tang, Y.H.; Ebong, A.; Honsberg, C.B.; Green, M.A.

    1994-12-31

    Rear surface effects in PERL solar cells can lead not only to degradation in the short circuit current and open circuit voltage, but also fill factor. Three mechanisms capable of changing the effective rear surface recombination velocity with injection level are identified, two associated with oxidized p-type surfaces, and the third with two dimensional effects associated with a rear floating junction. Each of these will degrade the fill factor if the range of junction biases corresponding to the rear surface transition, coincides with the maximum power point. Despite the identified non idealities, PERL cells with rear floating junctions (PERF cells) have achieved record open circuit voltages for silicon solar cells, while simultaneously achieving fill factor improvements relative to standard PERL solar cells. Without optimization, a record efficiency of 22% has been demonstrated for a cell with a rear floating junction. The results of both theoretical and experimental studies are provided.

  17. [Application of liquid chromatography in substitution of the radioimmunoassay technique in order to reduce residues generated in health services in research laboratory].

    PubMed

    Ribeiro Neto, Luciane M; Sugawara, Eduardo K; Verreschi, Ieda T N

    2008-10-01

    Designing a Health Care Service Waste Management Plan, according to the RDC 306 rules, is a responsibility of all those who produce such waste. Since radioimmunoassay (RIA) is one of the most employed techniques, we studied the impact of replacing this technique by liquid chromatography (HPLC) with regard to the reduction of the radioactive residues routinely produced by the Unifesp steroid laboratory. The residues produced by the determination of serum cortisol and 17 alpha-hydroxyprogesterone were classified, and those belonging to groups B and C were evaluated. We observed that, when RIA is used, chemical residues (group B) and radioactive waste (group C) are produced, whereas HPLC generates only chemical residues. Adequation of these techniques showed to be advantageous, by significantly reducing the time of analysis and mainly by eliminating and/or reducing the generation of radioactive waste, encouraging its application to other methodologies, as well as its adoption by other research units. PMID:19082306

  18. Distant view of rear and side (east) of building 256, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Distant view of rear and side (east) of building 256, along with the rear of building 257 (obstructed by truck), and side and rear of building 255. In the distance, the upper floors of the hospital, building 500, is visible. Camera station is next to building 264, looking south. - Fitzsimons General Hospital, Building 256, North side of East O'Niell Avenue, between Tenth & Twelfth Streets, Aurora, Adams County, CO

  19. 49 CFR 393.86 - Rear impact guards and rear end protection.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... rear impact guard that meets the requirements of Federal Motor Vehicle Safety Standard No. 223 (49 CFR... (49 CFR 571.224) in effect at the time the vehicle was manufactured. The requirements of paragraph (a... required by FMVSS No. 223 (49 CFR 571.223, S5.3). The label must be on the forward-facing surface of...

  20. 49 CFR 393.86 - Rear impact guards and rear end protection.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... rear impact guard that meets the requirements of Federal Motor Vehicle Safety Standard No. 223 (49 CFR... (49 CFR 571.224) in effect at the time the vehicle was manufactured. The requirements of paragraph (a... required by FMVSS No. 223 (49 CFR 571.223, S5.3). The label must be on the forward-facing surface of...

  1. 49 CFR 393.86 - Rear impact guards and rear end protection.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... rear impact guard that meets the requirements of Federal Motor Vehicle Safety Standard No. 223 (49 CFR... (49 CFR 571.224) in effect at the time the vehicle was manufactured. The requirements of paragraph (a... required by FMVSS No. 223 (49 CFR 571.223, S5.3). The label must be on the forward-facing surface of...

  2. 49 CFR 393.86 - Rear impact guards and rear end protection.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... rear impact guard that meets the requirements of Federal Motor Vehicle Safety Standard No. 223 (49 CFR... (49 CFR 571.224) in effect at the time the vehicle was manufactured. The requirements of paragraph (a... required by FMVSS No. 223 (49 CFR 571.223, S5.3). The label must be on the forward-facing surface of...

  3. 49 CFR 393.86 - Rear impact guards and rear end protection.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... rear impact guard that meets the requirements of Federal Motor Vehicle Safety Standard No. 223 (49 CFR... (49 CFR 571.224) in effect at the time the vehicle was manufactured. The requirements of paragraph (a... required by FMVSS No. 223 (49 CFR 571.223, S5.3). The label must be on the forward-facing surface of...

  4. Final report, Ames Mobile Laboratory Project: The development and operation of instrumentation in a mobile laboratory for in situ, real-time screening and characterization of soils using the laser ablation sampling technique

    SciTech Connect

    Anderson, M.S.; Braymen, S.D.

    1995-01-27

    The main focus of the Ames Laboratory`s Technology Integration Program, TIP, from May 1991 through December 1994 was the development, fabrication, and demonstration of a mobile instrumentation laboratory incorporating rapid in situ sampling systems for safe, rapid, and cost effective soil screening/characterization. The Mobile Demonstration Laboratory for Environmental Screening Technologies, MDLEST, containing the analysis instrumentation, along with surface and subsurface sampling probe prototypes employing the laser ablation sampling technique were chosen to satisfy the particular surface and subsurface soil characterization needs of the various Department of Energy facilities for determining the extent of heavy metal and radionuclide contamination. The MDLEST, a 44 foot long 5th wheel trailer, is easily configured for the analysis instrumentation and sampling system required for the particular site work. This mobile laboratory contains all of the utilities needed to satisfy the operating requirements of the various instrumentation installed. These utilities include, an electric generator, a chilled water system, process gases, a heating/air conditioning system, and computer monitoring and automatic operating systems. Once the MDLEST arrives at the job site, the instrumentation is aligned and calibration is completed, sampling and analysis operations begin. The sample is acquired, analyzed and the results reported in as little as 10 minutes. The surface sampling probe is used in two modes to acquire samples for analysis. It is either set directly on the ground over the site to be sampled, in situ sampling, or in a special fixture used for calibrating the sampling analysis system with standard soil samples, having the samples brought to the MDLEST. The surface sampling probe was used to in situ sample a flat concrete surface (nondestructively) with the ablated sample being analyzed by the instrumentation in the MDLEST.

  5. Laboratory tests of pulse shape discrimination techniques for correcting the effects of radiation damage in germanium coaxial detectors

    NASA Astrophysics Data System (ADS)

    Boggs, S. E.; Hull, E. L.; Lin, R. P.; Madden, N. W.; McBride, S.; Pehl, R. H.; Primbsch, J. H.; Slassi-Sennou, S.

    2000-04-01

    A reverse-electrode closed-end germanium coaxial detector was irradiated with 183-MeV neutrons to evaluate the value of Pulse Shape Discrimination (PSD) techniques in restoring the energy resolution and line shape of the radiation damaged detector. Two consecutive irradiations were performed for total fluences of 5.010 8 and 10.410 8 n/ cm2, with PSD tests performed after each irradiation. These irradiations degraded the energy resolution and line shapes; however, PSD corrections significantly restored the performance, even after severe damage. These PSD techniques delay and potentially eliminate, in some experimental situations, the need to anneal germanium detectors in damaging radiation environments.

  6. OBLIQUE VIEW, REAR ELEVATION, LOOKING NORTHEAST Mountain Home Air ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    OBLIQUE VIEW, REAR ELEVATION, LOOKING NORTHEAST - Mountain Home Air Force Base 1958 Senior Officers' Housing, General's Residence, Rabeni Street (originally Ivy Street), Mountain Home, Elmore County, ID

  7. BLDG 1 REAR (EAST) AND NORTH END Naval Magazine ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    BLDG 1 REAR (EAST) AND NORTH END - Naval Magazine Lualualei, Headquarters Branch, Administration Building, Between Constitution & Constellation Streets, east side of main quad, Pearl City, Honolulu County, HI

  8. GARAGE, SOUTH (REAR) AND EAST SIDE, LOOKING NORTHWEST Irvine ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    GARAGE, SOUTH (REAR) AND EAST SIDE, LOOKING NORTHWEST - Irvine Ranch Agricultural Headquarters, Carillo Tenant House, Southwest of Intersection of San Diego & Santa Ana Freeways, Irvine, Orange County, CA

  9. BLDG 102, REAR (WEST) AND NORTH END. Naval Magazine ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    BLDG 102, REAR (WEST) AND NORTH END. - Naval Magazine Lualualei, Headquarters Branch, Operational Storage Building, Fifteenth Street between Kolekole Road & Gilbert Street, Pearl City, Honolulu County, HI

  10. VIEW FACING NORTH SHOWING REAR ELEVATION OF MANUFACTURING BUILDING NO. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW FACING NORTH SHOWING REAR ELEVATION OF MANUFACTURING BUILDING NO. 1 AND SMALLER FURNACE STACK - Jeannette Glass Company, Chambers Avenue extension on Brush Creek, Jeannette, Westmoreland County, PA

  11. 7. INTERIOR, VIEW FROM ENTRANCE TOWARD ENCLOSED STAIRS AND REAR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. INTERIOR, VIEW FROM ENTRANCE TOWARD ENCLOSED STAIRS AND REAR DOOR - Mulliken-Spragins Tenant House, National Museum of American History, Smithsonian Institution, Washington, District of Columbia, DC

  12. An inter-laboratory comparison demonstrates that [1H]-NMR metabolite fingerprinting is a robust technique for collaborative plant metabolomic data collection

    PubMed Central

    Ward, Jane L.; Baker, John M.; Miller, Sonia J.; Deborde, Catherine; Maucourt, Mickael; Biais, Benoit; Rolin, Dominique; Moing, Annick; Moco, Sofia; Vervoort, Jacques; Lommen, Arjen; Schäfer, Hartmut; Humpfer, Eberhard

    2010-01-01

    In any metabolomics experiment, robustness and reproducibility of data collection is of vital importance. These become more important in collaborative studies where data is to be collected on multiple instruments. With minimisation of variance in sample preparation and instrument performance it is possible to elucidate even subtle differences in metabolite fingerprints due to genotype or biological treatment. In this paper we report on an inter laboratory comparison of plant derived samples by [1H]-NMR spectroscopy across five different sites and within those sites utilising instruments with different probes and magnetic field strengths of 9.4 T (400 MHz), 11.7 T (500 MHz) and 14.1 T (600 MHz). Whilst the focus of the study is on consistent data collection across laboratories, aspects of sample stability and the requirement for sample rotation within the NMR magnet are also discussed. Comparability of the datasets from participating laboratories was exceptionally good and the data were amenable to comparative analysis by multivariate statistics. Field strength differences can be adjusted for in the data pre-processing and multivariate analysis demonstrating that [1H]-NMR fingerprinting is the ideal technique for large scale plant metabolomics data collection requiring the participation of multiple laboratories. PMID:20526352

  13. An inter-laboratory comparison demonstrates that [H]-NMR metabolite fingerprinting is a robust technique for collaborative plant metabolomic data collection.

    PubMed

    Ward, Jane L; Baker, John M; Miller, Sonia J; Deborde, Catherine; Maucourt, Mickael; Biais, Benoit; Rolin, Dominique; Moing, Annick; Moco, Sofia; Vervoort, Jacques; Lommen, Arjen; Schäfer, Hartmut; Humpfer, Eberhard; Beale, Michael H

    2010-06-01

    In any metabolomics experiment, robustness and reproducibility of data collection is of vital importance. These become more important in collaborative studies where data is to be collected on multiple instruments. With minimisation of variance in sample preparation and instrument performance it is possible to elucidate even subtle differences in metabolite fingerprints due to genotype or biological treatment. In this paper we report on an inter laboratory comparison of plant derived samples by [(1)H]-NMR spectroscopy across five different sites and within those sites utilising instruments with different probes and magnetic field strengths of 9.4 T (400 MHz), 11.7 T (500 MHz) and 14.1 T (600 MHz). Whilst the focus of the study is on consistent data collection across laboratories, aspects of sample stability and the requirement for sample rotation within the NMR magnet are also discussed. Comparability of the datasets from participating laboratories was exceptionally good and the data were amenable to comparative analysis by multivariate statistics. Field strength differences can be adjusted for in the data pre-processing and multivariate analysis demonstrating that [(1)H]-NMR fingerprinting is the ideal technique for large scale plant metabolomics data collection requiring the participation of multiple laboratories. PMID:20526352

  14. Low-level measuring techniques for neutrons: High accuracy neutron source strength determination and fluence rate measurement at an underground laboratory

    NASA Astrophysics Data System (ADS)

    Zimbal, Andreas; Degering, Detlev; Reginatto, Marcel; Schuhmacher, Helmut; Wiegel, Burkhard; Zuber, Kai

    2013-08-01

    We report on measuring techniques for neutrons that have been developed at the Physikalisch-Technische Bundesanstalt (PTB), the German National Metrology Institute. PTB has characterized radioactive sources used in the BOREXINO and XENON100 experiments. For the BOREXINO experiment, a 228Th gamma radiation source was required which would not emit more than 10 neutrons per second. The determination of the neutron emission rate of this specially designed 228Th source was challenging due to the low neutron emission rate and because the ratio of neutron to gamma radiation was expected to be extremely low, of the order of 10-6. For the XENON100 detector, PTB carried out a high accuracy measurement of the neutron emission rate of an AmBe source. PTB has also done measurements in underground laboratories. A two month measurement campaign with a set of 3He-filled proportional counters was carried out in PTB's former UDO underground laboratory at the Asse salt mine. The aim of the campaign was to determine the intrinsic background of detectors, which is needed for the analysis of data taken in lowintensity neutron fields. At a later time, PTB did a preliminary measurement of the neutron fluence rate at the underground laboratory Felsenkeller operated by VKTA. By taking into account data from UDO, Felsenkeller, and detector calibrations made at the PTB facility, it was possible to estimate the neutron fluence rate at the Felsenkeller underground laboratory.

  15. Low-level measuring techniques for neutrons: High accuracy neutron source strength determination and fluence rate measurement at an underground laboratory

    SciTech Connect

    Zimbal, Andreas; Reginatto, Marcel; Schuhmacher, Helmut; Wiegel, Burkhard; Degering, Detlev; Zuber, Kai

    2013-08-08

    We report on measuring techniques for neutrons that have been developed at the Physikalisch-Technische Bundesanstalt (PTB), the German National Metrology Institute. PTB has characterized radioactive sources used in the BOREXINO and XENON100 experiments. For the BOREXINO experiment, a {sup 228}Th gamma radiation source was required which would not emit more than 10 neutrons per second. The determination of the neutron emission rate of this specially designed {sup 228}Th source was challenging due to the low neutron emission rate and because the ratio of neutron to gamma radiation was expected to be extremely low, of the order of 10{sup −6}. For the XENON100 detector, PTB carried out a high accuracy measurement of the neutron emission rate of an AmBe source. PTB has also done measurements in underground laboratories. A two month measurement campaign with a set of {sup 3}He-filled proportional counters was carried out in PTB's former UDO underground laboratory at the Asse salt mine. The aim of the campaign was to determine the intrinsic background of detectors, which is needed for the analysis of data taken in lowintensity neutron fields. At a later time, PTB did a preliminary measurement of the neutron fluence rate at the underground laboratory Felsenkeller operated by VKTA. By taking into account data from UDO, Felsenkeller, and detector calibrations made at the PTB facility, it was possible to estimate the neutron fluence rate at the Felsenkeller underground laboratory.

  16. Lean Management and Six-Sigma yield big gains in hospital's immediate response laboratory. Quality improvement techniques save more than $400,000.

    PubMed

    Sunyog, Martha

    2004-01-01

    DSI Laboratories has used Lean and Six-Sigma methodologies to systematically eliminate waste and reduce variation in its hospital clinical laboratory. The net result was a savings of more than $400,000 in the first year. After mapping its process, the laboratory found that phlebotomists were submitting samples in large batches, which created an early-morning flood of specimens. Switching to single-piece workflow and distributing the workload more evenly allowed DSI to cover the same number of patients with two to three phlebotomists instead of 12. By establishing a more efficient workflow process within the laboratory, a single technologist could quickly move between stations and perform those tests that made up 80% of the work volume. New inventory management techniques reduced both excess inventory and the risk of shortages. Cost savings for the first year were: Reduced overtime spending by 60% ($78,000), Reassigned six phlebotomists for an annual savings of $160,000, 4.5 fewer technologist positions for a savings of $250,000. PMID:15493100

  17. A Solution on Identification and Rearing Files Insmallhold Pig Farming

    NASA Astrophysics Data System (ADS)

    Xiong, Benhai; Fu, Runting; Lin, Zhaohui; Luo, Qingyao; Yang, Liang

    In order to meet government supervision of pork production safety as well as consumeŕs right to know what they buy, this study adopts animal identification, mobile PDA reader, GPRS and other information technologies, and put forward a data collection method to set up rearing files of pig in smallhold pig farming, and designs related metadata structures and its mobile database, and develops a mobile PDA embedded system to collect individual information of pig and uploading into the remote central database, and finally realizes mobile links to the a specific website. The embedded PDA can identify both a special pig bar ear tag appointed by the Ministry of Agricultural and a general data matrix bar ear tag designed by this study by mobile reader, and can record all kinds of inputs data including bacterins, feed additives, animal drugs and even some forbidden medicines and submitted them to the center database through GPRS. At the same time, the remote center database can be maintained by mobile PDA and GPRS, and finally reached pork tracking from its origin to consumption and its tracing through turn-over direction. This study has suggested a feasible technology solution how to set up network pig electronic rearing files involved smallhold pig farming based on farmer and the solution is proved practical through its application in the Tianjińs pork quality traceability system construction. Although some individual techniques have some adverse effects on the system running such as GPRS transmitting speed now, these will be resolved with the development of communication technology. The full implementation of the solution around China will supply technical supports in guaranteeing the quality and safety of pork production supervision and meet consumer demand.

  18. Rearing in Seawater Mesocosms Improves the Spawning Performance of Growth Hormone Transgenic and Wild-Type Coho Salmon

    PubMed Central

    Leggatt, Rosalind A.; Hollo, Tanya; Vandersteen, Wendy E.; McFarlane, Kassandra; Goh, Benjamin; Prevost, Joelle; Devlin, Robert H.

    2014-01-01

    Growth hormone (GH) transgenes can significantly accelerate growth rates in fish and cause associated alterations to their physiology and behaviour. Concern exists regarding potential environmental risks of GH transgenic fish, should they enter natural ecosystems. In particular, whether they can reproduce and generate viable offspring under natural conditions is poorly understood. In previous studies, GH transgenic salmon grown under contained culture conditions had lower spawning behaviour and reproductive success relative to wild-type fish reared in nature. However, wild-type salmon cultured in equal conditions also had limited reproductive success. As such, whether decreased reproductive success of GH transgenic salmon is due to the action of the transgene or to secondary effects of culture (or a combination) has not been fully ascertained. Hence, salmon were reared in large (350,000 L), semi-natural, seawater tanks (termed mesocosms) designed to minimize effects of standard laboratory culture conditions, and the reproductive success of wild-type and GH transgenic coho salmon from mesocosms were compared with that of wild-type fish from nature. Mesocosm rearing partially restored spawning behaviour and success of wild-type fish relative to culture rearing, but remained lower overall than those reared in nature. GH transgenic salmon reared in the mesocosm had similar spawning behaviour and success as wild-type fish reared in the mesocosm when in full competition and without competition, but had lower success in male-only competition experiments. There was evidence of genotype×environmental interactions on spawning success, so that spawning success of transgenic fish, should they escape to natural systems in early life, cannot be predicted with low uncertainty. Under the present conditions, we found no evidence to support enhanced mating capabilities of GH transgenic coho salmon compared to wild-type salmon. However, it is clear that GH transgenic salmon are capable of successful spawning, and can reproduce with wild-type fish from natural systems. PMID:25133780

  19. Minimum survival rates for Mississippi sandhill cranes: a comparison of hand-rearing and parent-rearing

    USGS Publications Warehouse

    Ellis, D.H.; Gee, G.F.; Olsen, G.H.; Hereford, Scott G.; Nicolich, J.M.; Thomas, N.J.; Nagendran, M.

    2001-01-01

    Hand-reared (56) and parent-reared (76) juvenile Mississippi sandhill cranes (Grus canadensis pulla) were produced at the Patuxent Wildlife Research Center (Patuxent), Laurel, Maryland over a 4-year period (1989-92) and released at the Mississippi Sandhill Crane National Wildlife Refuge (Refuge), Gautier, Mississippi in a controlled experiment. Hand-reared survival rates proved higher than for parent-reared survival for each time category: 6 months, 86% versus 75%; 1 year, 77% versus 68%; 2 years 66% versus 53%; 3 years, 55% versus 43%: partial data for fourth and filth years were 57% versus 31% and 48% versus 37%.

  20. Fitness and field performance of a mass-reared biological control agent, Rhinoncomimus latipes (Coleoptera: Curculionidae).

    PubMed

    Hough-Goldstein, J; Stout, A R; Schoenstein, J A

    2014-08-01

    Rhinoncomimus latipes Korotyaev (Coleoptera: Curculionidae), a biological control agent of mile-a-minute weed, Persicaria perfoliata (L.) H. Gross, has been mass reared with no infusion of new genetic material for 8-9 yr (at least 24-36 generations), while insects from the same genetic stock have been subject to field conditions in North America for that same period of time. Our main objective was to compare the laboratory population with the field population (and in 1 yr with a Chinese field population) to determine whether genetic changes had occurred, especially ones that may reduce the effectiveness of the laboratory population when released in the field. The laboratory insects laid more eggs and had reduced survival compared with field weevils in several comparisons, and had reduced responsiveness to cues that induce reproductive diapause. Exposure to older plants had the greatest effect on induction of reproductive diapause in both laboratory and field weevils, with effects of daylength and temperature less pronounced. At least a portion of the laboratory weevil population overwintered successfully. Results suggest that it is not necessary to add wild-type genetic material to the rearing colony at this time. PMID:25182614

  1. Behavioural responses of hatchery-reared and wild cod Gadus morhua to mechano-acoustic predator signals.

    PubMed

    Meager, J J; Rodewald, P; Domenici, P; Fernö, A; Järvi, T; Skjaeraasen, J E; Sverdrup, G K

    2011-05-01

    The behavioural responses of wild (predator-experienced) and hatchery-reared (predator-naive) cod Gadus morhua to standardized mechano-acoustic (MA) stimuli were compared in the laboratory. Wild fish responded mainly with freezing and fast-start escapes away from the stimulus, whereas hatchery-reared fish often ignored or approached the stimulus. Wild fish also had stronger responses, turning faster during escapes and reducing activity immediately after the stimulus. Both fish types were less active on a 'risky' bare substratum after the stimulus. The antipredator responses of wild fish were consistent to repeated stimuli, whereas hatchery-reared fish that had generally only encountered harmless stimuli showed more variable responses with lower repeatability. This suggests that experience plays a role in shaping the behavioural response of fishes to MA stimuli. PMID:21539552

  2. Comparing growth of pork- and venison-reared Phormia regina (Diptera: Calliphoridae) for the application of forensic entomology to wildlife poaching.

    PubMed

    Wilson, J M; Lafon, N W; Kreitlow, K L; Brewster, C C; Fell, R D

    2014-09-01

    Laboratory rearing of Phormia regina Meigen larvae on pork and venison was conducted as part of a study to determine whether forensic entomology approaches can be used in wildlife poaching investigations. Larvae were reared at 30 degrees C, 75% relative humidity, and a photoperiod of 14:10 (L:D) h on pork or venison diets, and samples were collected every 8 h until >90% of the maggots reached the third-instar wandering or prepupal stage. Significant differences were found in the distribution of lengths of the third instar and combined instars for maggots reared on the two different meat sources. Maggots reared on venison reached the prepupal wandering stage significantly faster (approximately 6 h) compared with maggots on the pork diet. Mean adult weight and wing length of venison-reared flies were significantly greater than for flies reared on pork. The lower crude fat content of venison appears to make this meat source a more suitable medium than pork for larvae of P. regina. The difference in growth rate could introduce error into PMImin estimations from third-instar maggots in deer poaching cases if estimates are based on data from studies in which maggots were reared on pork. PMID:25276938

  3. The Fundamentals of Flying: Simple and Inexpensive Strategies for Employing Drosophila Genetics in Neuroscience Teaching Laboratories

    PubMed Central

    Pulver, Stefan R.; Berni, Jimena

    2012-01-01

    Drosophila researchers have developed a powerful suite of genetic techniques for studying the neural basis of animal behavior. Many of these tools can be exported to neuroscience teaching laboratories (Berni et al., 2010; Pulver et al., 2011a,b), but often neuroscience educators lack the basic knowledge and resources to obtain, generate and rear transgenic fruit flies on their own. Fly researchers in turn may take for granted resources that are readily available in research laboratories, but out of reach for educators. Our goal is to provide a primer for neuroscience educators who want to incorporate Drosophila genetics into their teaching, but have limited knowledge of fruit fly genetics, and/or small budgets. First we review the available methods for manipulating gene expression in Drosophila. Then we provide educators with blueprints for obtaining transgenic animals tailored for specific types of teaching modules. We outline simple techniques for rearing transgenic Drosophila, performing genetic crosses, and preparing a teaching laboratory without the use of expensive animal-care facilities. Overall, we try to break down the practical barriers educators may face when integrating modern neurogenetic experiments into teaching laboratories. PMID:23493248

  4. LDRD ER Final Report: Recreating Planetary Cores in the Laboratory: New Techniques to Extremely High Density States

    SciTech Connect

    Collins, G; Celliers, P; Hicks, D; Cauble, R; Bradley, D; MacKinnon, A; Moon, S; Young, D; Chau, R; Eggert, J; Willi, P; Pasley, J; Jeanloz, R; Lee, K; Bennedetti, R; Koenig, M; Benuzzi-Mounaix, A; Batani, D; Loubeyre, P; Hubbard, W

    2003-02-07

    An accurate equation of state (EOS) for planetary constituents at extreme conditions is the key to any credible model of planets or low mass stars. However, very few materials have their high pressure (>few Mbar) EOS experimentally validated, and even then, only on the principal Hugoniot. For planetary and stellar interiors, compression occurs from gravitational force so that material states follow a line of isotropic compression (ignoring phase separation) to ultra-high densities. An example of the hydrogen phase space composing Jupiter and one particular Brown Dwarf is shown. At extreme densities, material states are predicted to have quite unearthly properties such as high temperature superconductivity and low temperature fusion. High density experiments on Earth are achieved with either static compression techniques (i.e. diamond anvil cells) or dynamic compression techniques using large laser facilities, gas guns, or explosives. The ultimate goal of this multi-directorate and multi-institutional proposal was to develop techniques that will enable us to understand material states that previously only existed at the core of giant planets, stars, or speculative theories. Our effort was a complete success, meeting all of the objectives set out in our proposals. First we focused on developing accurate Hugoniot techniques to be used for constraining the equation of state at high pressure/temperature. We mapped out an accurate water EOS and measured that the ionic->electronic conduction transition occurs at lower pressures than models predict. These data and their impact are fully described in the first enclosed paper ''The Equation of State and Optical Properties of Water Compressed by Strong Shock Waves.'' Currently models used to construct planetary isentropes are constrained by only the planet radius, outer atmospheric spectroscopy, and space probe gravitational moment and magnetic field data. Thus these data, which provide rigid constraints to these models, will have a significant impact on a broad community of planetary and condensed matter scientists, as well as our fundamental understanding of the giant planets. We then developed and tested precompressed and multiple shock techniques on water. Scientists around the world have teamed with us to conduct these complex and seminal high density experiments which allow access to the extreme core states of giant plants. Double shock experiments using a variety of anvils to compress water to densities higher and temperatures lower than accessible by single shock Hugoniot techniques. First a clear determination of the EOS and optical properties of the anvils needed to be measured. These properties for LiF and A1203 are written up in the second attached article, ''Shock-Induced Transformation of Sapphire and Lithium Fluoride into Semiconducting Liquids.'' An example double shock data record for water is shown. This data is being written up for publication.

  5. Colonisation and mass rearing: learning from others.

    PubMed

    Benedict, Mark Q; Knols, Bart G J; Bossin, Hervé C; Howell, Paul I; Mialhe, Eric; Caceres, Carlos; Robinson, Alan S

    2009-01-01

    Mosquitoes, just as other insects produced for the sterile insect technique (SIT), are subjected to several unnatural processes including laboratory colonisation and large-scale factory production. After these processes, sterile male mosquitoes must perform the natural task of locating and mating with wild females. Therefore, the colonisation and production processes must preserve characters necessary for these functions. Fortunately, in contrast to natural selection which favours a suite of characteristics that improve overall fitness, colonisation and production practices for SIT strive to maximize only the few qualities that are necessary to effectively control populations. However, there is considerable uncertainty about some of the appropriate characteristics due to the lack of data. Development of biological products for other applications suggest that it is possible to identify and modify competitiveness characteristics in order to produce competitive mass produced sterile mosquitoes. This goal has been pursued--and sometimes achieved--by mosquito colonisation, production, and studies that have linked these characteristics to field performance. Parallels are drawn to studies in other insect SIT programmes and aquaculture which serve as vital technical reference points for mass-production of mosquitoes, most of whose development occurs--and characteristics of which are determined--in an aquatic environment. Poorly understood areas that require further study are numerous: diet, mass handling and genetic and physiological factors that influence mating competitiveness. Compromises in such traits due to demands to increase numbers or reduce costs, should be carefully considered in light of the desired field performance. PMID:19917074

  6. Colonisation and mass rearing: learning from others

    PubMed Central

    Benedict, Mark Q; Knols, Bart GJ; Bossin, Hervé C; Howell, Paul I; Mialhe, Eric; Caceres, Carlos; Robinson, Alan S

    2009-01-01

    Mosquitoes, just as other insects produced for the sterile insect technique (SIT), are subjected to several unnatural processes including laboratory colonisation and large-scale factory production. After these processes, sterile male mosquitoes must perform the natural task of locating and mating with wild females. Therefore, the colonisation and production processes must preserve characters necessary for these functions. Fortunately, in contrast to natural selection which favours a suite of characteristics that improve overall fitness, colonisation and production practices for SIT strive to maximize only the few qualities that are necessary to effectively control populations. However, there is considerable uncertainty about some of the appropriate characteristics due to the lack of data. Development of biological products for other applications suggest that it is possible to identify and modify competitiveness characteristics in order to produce competitive mass produced sterile mosquitoes. This goal has been pursued - and sometimes achieved - by mosquito colonisation, production, and studies that have linked these characteristics to field performance. Parallels are drawn to studies in other insect SIT programmes and aquaculture which serve as vital technical reference points for mass-production of mosquitoes, most of whose development occurs - and characteristics of which are determined - in an aquatic environment. Poorly understood areas that require further study are numerous: diet, mass handling and genetic and physiological factors that influence mating competitiveness. Compromises in such traits due to demands to increase numbers or reduce costs, should be carefully considered in light of the desired field performance. PMID:19917074

  7. Rear semicircular section of the highlift pumping station basement with ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Rear semi-circular section of the high-lift pumping station basement with remnants of the piping systems and suction wells at rear wall. - Robert B. Morse Water Filtration Plant, 10700 and 10701 Columbia Pike, Silver Spring, Montgomery County, MD

  8. North rear, oblique view to the southeast, showing the east ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    North rear, oblique view to the southeast, showing the east wing and rear wall construction. Note the outline of the former windows beneath the current small aluminum-frame windows - Wellton-Mohawk Irrigation System, Building No. 16 A-B (Duplex), 30652 & 30654 Wellton-Mohawk Drive, Wellton, Yuma County, AZ

  9. DOORS AT REAR KITCHEN WALL, LOOKING SOUTH; LEFT DOOR LEADS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DOORS AT REAR KITCHEN WALL, LOOKING SOUTH; LEFT DOOR LEADS TO SMALL PANTRY, EAST DOOR AND DINING ROOM; 2ND LEFT DOOR LEADS TO BASEMENT; 3RD LEFT DOOR LEADS TO 2ND FLOOR VIA REAR STAIRS; RIGHT DOOR LEADS TO PANTRY, DINING ROOM - Fort Leavenworth, Building No. 18, 16-18 Sumner Place, Leavenworth, Leavenworth County, KS

  10. 14 CFR 23.369 - Rear lift truss.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Rear lift truss. 23.369 Section 23.369 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS... lift truss. (a) If a rear lift truss is used, it must be designed to withstand conditions of...

  11. 14 CFR 23.369 - Rear lift truss.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 1 2013-01-01 2013-01-01 false Rear lift truss. 23.369 Section 23.369 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS... lift truss. (a) If a rear lift truss is used, it must be designed to withstand conditions of...

  12. 14 CFR 23.369 - Rear lift truss.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Rear lift truss. 23.369 Section 23.369 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS... lift truss. (a) If a rear lift truss is used, it must be designed to withstand conditions of...

  13. 14 CFR 23.369 - Rear lift truss.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Rear lift truss. 23.369 Section 23.369 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS... lift truss. (a) If a rear lift truss is used, it must be designed to withstand conditions of...

  14. 14 CFR 23.369 - Rear lift truss.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 1 2011-01-01 2011-01-01 false Rear lift truss. 23.369 Section 23.369 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS... lift truss. (a) If a rear lift truss is used, it must be designed to withstand conditions of...

  15. 3. First floor of rear ell: view of exposed framing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. First floor of rear ell: view of exposed framing on north wall, floor, and ceiling, looking north - McCrone House, Rear Ell, New Castle Hundred, 1 mile South of intersection of U.S. Route 13 & Route 40, west of Route 13, New Castle, New Castle County, DE

  16. 2. Elevation of south side of dwelling and rear ell ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Elevation of south side of dwelling and rear ell - McCrone House, Rear Ell, New Castle Hundred, 1 mile South of intersection of U.S. Route 13 & Route 40, west of Route 13, New Castle, New Castle County, DE

  17. Employed and Nonemployed Mothers: Differences in Parental Child Rearing Practices.

    ERIC Educational Resources Information Center

    Mills, Belen C.; Stevens, Ann

    1985-01-01

    Introduces research comparing changes in the child rearing practices of employed and nonemployed mothers of four-year-old children. Also discusses the mother's occupational role as it influences the parent's attitudes in child rearing practices. No significant differences were found between the employed and nonemployed mothers on six Child Rearing…

  18. 2. VIEW LOOKING NORTH OF GUARDLOCK (LEFT REAR), DUNDEE DAM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW LOOKING NORTH OF GUARDLOCK (LEFT REAR), DUNDEE DAM (RIGHT-CENTER REAR), AND REMOVED SITE OF TOWPATH (FOREGROUND) DURING HYDROPOWER FACILITY INSTALLATION - Dundee Canal, Headgates, Guardlock & Uppermost Section, 250 feet northeast of Randolph Avenue, opposite & in line with East Clifton Avenue, Clifton, Passaic County, NJ

  19. Particle Imaging Velocimetry Technique Development for Laboratory Measurement of Fracture Flow Inside a Pressure Vessel Using Neutron Imaging

    SciTech Connect

    Polsky, Yarom; Bingham, Philip R; Bilheux, Hassina Z; Carmichael, Justin R

    2015-01-01

    This paper will describe recent progress made in developing neutron imaging based particle imaging velocimetry techniques for visualizing and quantifying flow structure through a high pressure flow cell with high temperature capability (up to 350 degrees C). This experimental capability has great potential for improving the understanding of flow through fractured systems in applications such as enhanced geothermal systems (EGS). For example, flow structure measurement can be used to develop and validate single phase flow models used for simulation, experimentally identify critical transition regions and their dependence on fracture features such as surface roughness, and study multiphase fluid behavior within fractured systems. The developed method involves the controlled injection of a high contrast fluid into a water flow stream to produce droplets that can be tracked using neutron radiography. A description of the experimental setup will be provided along with an overview of the algorithms used to automatically track droplets and relate them to the velocity gradient in the flow stream. Experimental results will be reported along with volume of fluids based simulation techniques used to model observed flow.

  20. IMPROVED WELL PLUGGING EQUIPMENT AND WASTE MANGEMENT TECHNIQUES EXCEED ALARA GOALS AT THE OAK RIDGE NATIONAL LABORATORY

    SciTech Connect

    Whiteside, R.; Pawlowicz, R.; Whitehead, L.; Arnseth, R.

    2002-02-25

    In 2000, Bechtel Jacobs Company LLC (BJC) contracted Tetra Tech NUS, Inc. (TtNUS) and their sub-contractor, Texas World Operations, Inc. (TWO), to plug and abandon (P&A) 111 wells located in the Melton Valley area of Oak Ridge National Laboratory (ORNL). One hundred and seven of those wells were used to monitor fluid movement and subsurface containment of the low level radioactive liquid waste/grout slurry that was injected into the Pumpkin Valley Shale Formation, underlying ORNL. Four wells were used as hydrofracture injection wells to emplace the waste in the shale formation. Although the practice of hydrofracturing was and is considered by many to pose no threat to human health or the environment, the practice was halted in 1982 after the Federal Underground Injection Control regulations were enacted by United States Environmental Protection Agency (USEPA) making it necessary to properly close the wells. The work is being performed for the United States Department of Energy Oak Ridge Operations (DOE ORO). The project team is using the philosophy of minimum waste generation and the principles of ALARA (As Low As Reasonably Achievable) as key project goals to minimize personnel and equipment exposure, waste generation, and project costs. Achievement of these goals was demonstrated by the introduction of several new pieces of custom designed well plugging and abandonment equipment that were tested and used effectively during field operations. Highlights of the work performed and the equipment used are presented.

  1. Hot flue gas filtration: Laboratory tests and projected cost of a combined sorbent injection/filtration technique

    SciTech Connect

    Levendis, Y.A.; Shemwell, B.E.; Atal, A.

    2000-07-01

    This manuscript provides an update on the technology of flue gas filtration at high temperatures. In this technology the synergistic effects of sorbent injection and filtration are exploited. Calcium-based (or sodium-based) sorbents are sprayed in the post-flame zone of a furnace. The sorbent reacts with sulfur and chlorine in the furnace effluent to form stable salts of calcium. The partially-reacted sorbent is then collected in the filter, which is placed downstream of the sorbent injection point. The sorbent continues to react in the filter for a prolonged period of time. The primary advantage of this technique is that both the likelihood of contact and the period of contact between the solid sorbent particles and the gaseous pollutants (such as SO{sub 2}, H{sub 2}S, HCl, etc.) increase, since reaction takes place in the furnace upstream of the filter as well as inside the filter itself. Hence, the sorbent utilization (i.e., the conversion of sorbent CaO to CaSO{sub 4}, CaSO{sub 3}, CaCl{sub 2}, etc.) increases significantly. The filter is cleaned with periodic aerodynamic regeneration (backpulsing). Using this technique, most of the pollutants, such as SO{sub 2}, H{sub 2}S, HCl and particulate (soot, ash and tar) can be removed from the effluent. Any unburned carbon in the ash may be completely oxidized in the filter. Formation of dioxins in the effluent at lower temperatures should be minimized, since their precursors are captured by the filter. Results show That the sorbent/filter method appears to be less expensive than current technologies, on both capital cost ($/kW) and levelized coat ($/ton pollutant removed) bases, if a limestone/coal mixture is used as the sorbent for the fossil fuel plants. Capital costs for the filter/sorbent combination should be under $63/kW for a new plant, while those for a state of the art compounded wet limestone FGD/SCR/fabric filter at $250--400/kW. Since the existing technologies are designed for removing one pollutant at a time both their cost and space requirement are much higher than those of the technique discussed herein. Moreover, the levelized costs are projected to be as low as $103/ton of combined pollutant SO{sub 2} and NO{sub x} and particulates removed.

  2. Autonomy, Educational Plans, and Self-Esteem in Institution-Reared and Home-Reared Teenagers in Estonia

    ERIC Educational Resources Information Center

    Tulviste, Tiia

    2011-01-01

    The study examines autonomy, self-esteem, and educational plans for the future of 109 institution-reared and 106 home-reared teenagers (15-19 years). Teenagers were asked to complete the Teen Timetable Scale (Feldman & Rosenthal), two Emotional Autonomy Scales (Steinberg & Silverberg), the Rosenberg Self-Esteem Scale, and answer questions about…

  3. Behavioral and hormonal effects of attachment object separation in surrogate-peer-reared and mother-reared infant rhesus monkeys.

    PubMed

    Meyer, J S; Novak, M A; Bowman, R E; Harlow, H F

    1975-09-01

    Mother-reared and surrogate-peer-reared rhesus monkeys were separated from their respective attachment objects at 6 months of age and tested for the following 9 weeks to determine their home-cage behavior and their pituitary-adrenocortical responses to stress. Both groups displayed a strong immediate behavioral response to separation which was characterized by increased vocalization, increased locomotion, and decreased self-play. However, the surrogate-peer-reared infants showed a subsequent recovery in their levels of self-play whereas the mother-reared infants instead developed stereotypic behavior patterns such as repetitive pacing. The 2 groups displayed similar plasma cortisol responses to weekly sessions in an apparatus equipped with animated toy "monsters". Mother-reared but not surrogate-peer-reared subjects, however, also manifested elevated cortisol levels when an animal in an adjacent cage was captured and removed for stress testing. Mother-reared infant monkeys thus responded in a stronger and more prolonged manner to the loss of their attachment object than surrogate-peer-reared infants. These results suggest that infant rhesus monkeys form stronger attachments to monkey mothers than to inanimate surrogate mothers, a phenomenon which has not been as clearly demonstrated using other indices of attachment strength. PMID:817950

  4. Genetic and environmental influences on eating behavior - a study of twin pairs reared apart or reared together

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study examined the relative influence of genetic versus environmental factors on specific aspects of eating behavior. Adult monozygotic twins (22 pairs and 3 singleton reared apart, 38 pairs and 9 singleton reared together, age 18-76 years, BMI 17-43 kg/m2) completed the Three Factor Eating Que...

  5. A Comparison of Value Preferences and Attitudes toward Collectivism of Institution-Reared and Home-Reared Teenagers

    ERIC Educational Resources Information Center

    Tulviste, Tiia; Gutman, Piret

    2003-01-01

    The main purpose of this study was to shed some light on the effect of socialization context (home vs. orphanage) on gender-specific value acquisition. With this aim value preferences and collectivistic attitudes of institution-reared and home-reared teenagers (14-20 yrs.) were compared. Teenagers from ordinary homes were found to score high on…

  6. Fatal cytauxzoonosis in captive-reared lions in Brazil.

    PubMed

    Peixoto, P V; Soares, C O; Scofield, A; Santiago, C D; França, T N; Barros, S S

    2007-04-30

    Fatal cytauxzoonosis is described in a captive reared lioness (Panthera leo) and its 6-month-old cub. Clinical signs in the lioness included loss of weight, depression, anaemia, loss of hair, dark discolored urine, tachypnoea, nystagmus, deaphness and staggering gait. The cub died after a short period of depression. In the lioness, laboratory examination revealed normochromic normocytic anaemia, neutrophilia, lymphopenia, monocytosis, eosinopenia, thrombocytopenia, proteinuria, pyuria, haematuria and increased. At necropsy the lioness showed marked pulmonary edema and slight gelatinous translucent edema in the mediastinum, petechiae and echymosis disseminated in the serosae, and the intestinal content was red and semiliquid. The cub presented hemothorax, endocardial and pulmonary edema, petechiae in the cardiac serosae, hepatic and splenic congestion and segments of the small intestine with blood stained fluid contents and reddish mesenteric lymph nodes. Histopathological examination of liver, spleen, heart, lungs, intestines, pancreas, mesenteric lymph nodes, kidneys, skeletal muscle, brain and skin revealed large number of intravascular macrophages with their cytoplasm filled with various schizogonic stages of a Theileriidae. Electron microscopy confirmed the presence of schizonts in endothelial-associated macrophages. The diagnosis was established by the finding of the pathognomonic schizonts in macrophages within blood vessels in several organs and tissues from both lions. This is the first report of feline cytauxzoonosis in P. leo and of a confirmed infection by Cytauxzoon felis in felidae in South America. PMID:17306459

  7. A genomic perspective towards assessing quality of mass-reared SIT flies used in Mediterranean fruit fly eradication in California

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Temperature sensitive lethal (tsl) mutants of the tephritid C. capitata, are extensively used in control programmes involving sterile insect technique. These flies are artificially reared and treated with ionizing radiation to render males sterile for further release into the field to compete with w...

  8. Effect of rearing strategy and gamma radiation on fecundity and fertility of codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The codling moth, Cydia pomonella (L.), is a serious pest of pome fruit worldwide. In an effort to reduce the use of pesticides to control this pest, the Sterile Insect Technique (SIT) is being used or considered as an integrated pest control tactic. Rearing codling moths through diapause has been...

  9. Dissociative electron attachment to acetaldehyde, CH3CHO. A laboratory study using the velocity map imaging technique.

    PubMed

    Szymańska, Ewelina; Prabhudesai, Vaibhav S; Mason, Nigel J; Krishnakumar, E

    2013-01-21

    A detailed experimental investigation of the dissociative electron attachment (DEA) process to acetaldehyde, CH(3)CHO is presented. To investigate this process we use a time of flight spectrometer coupled with the velocity slice imaging technique. DEA in CH(3)CHO is found to lead to the formation of CH(3)(-), O(-), OH(-), C(2)H(-), C(2)HO(-) and CH(3)CO(-) anionic products produced through scattering resonances in the electron energy range of 6 to 13 eV. Of these product ions only O(-) is formed with any measurable kinetic energy distribution indicating a two-body dissociation process. CH(3)CO(-), although formed with very low kinetic energy, shows anisotropy in the velocity slice image, indicating ejection of the H atom in the 180° direction with respect to the electron beam. The low kinetic energy distributions and absence of any anisotropy in the angular distributions of the other product ions indicate that they are formed through multiple fragmentation of the transient molecular negative ion. The angular distribution of O(-) is analysed in terms of the various partial waves. PMID:23212656

  10. Captive Rearing Initiative for Salmon River Chinook Salmon, 1998-1999 Progress Report.

    SciTech Connect

    Hassemer, Peter F.

    2001-04-01

    During 1999, the Idaho Department of Fish and Game (IDFG) continued developing techniques for the captive rearing of chinook salmon Oncorhynchus tshawytscha. Techniques under development included protocols for rearing juveniles in freshwater and saltwater hatchery environments, and fieldwork to collect brood year 1998 and 1999 juveniles and eggs and to investigate the ability of these fish to spawn naturally. Fish collected as juveniles were held for a short time at the Sawtooth Fish Hatchery and later transferred to the Eagle Fish Hatchery for rearing. Eyed-eggs were transferred immediately to the Eagle Fish Hatchery where they were disinfected and reared by family groups. When fish from either collection method reached approximately 60 mm, they were PIT tagged and reared separately by brood year and source stream. Sixteen different groups were in culture at IDFG facilities in 1999. Hatchery spawning activities of captive-reared chinook salmon produced eyed-eggs for outplanting in streamside incubation chambers in the West Fork Yankee Fork Salmon River (N=2,297) and the East Fork Salmon River (N=1,038). Additionally, a number of these eggs were maintained at the Eagle Fish Hatchery to ensure adequate brood year 1999 representation from these systems, and produced 279 and 87 juveniles from the West Fork Yankee Fork and East Fork Salmon River, respectively. Eyed-eggs were not collected from the West Fork Yankee Fork due to low adult escapement. Brood year 1998 juveniles were collected from the Lemhi River (N=191), West Fork Yankee Fork Salmon River (N=229), and East Fork Salmon River (N=185). Additionally, brood year 1999 eyed-eggs were collected from the Lemhi River (N=264) and East Fork Salmon River (N=143). Sixty-two and seven maturing adults were released into Bear Valley Creek (Lemhi River system) and the East Fork Salmon River, respectively, for spawning evaluation in 1999. Nine female carcasses from Bear Valley Creek were examined for egg retention, and of these five were spawned out, one was partially spawned, and three died before depositing eggs. However, much of the spawning related behavior observed involved female chinook salmon paired with male bull trout Salvelinus confluentus. Two female carcasses from the East Fork Salmon River were recovered and examined for egg retention. One of these had spawned and one had not.

  11. Captive Rearing Initiative for Salmon River Chinook Salmon, 1999 Progress Report.

    SciTech Connect

    Hassemer, Peter F.

    2001-04-01

    During 1999, the Idaho Department of Fish and Game (IDFG) continued developing techniques for the captive rearing of chinook salmon Oncorhynchus tshawytscha. Techniques under development included protocols for rearing juveniles in freshwater and saltwater hatchery environments, and fieldwork to collect brood year 1998 and 1999 juveniles and eggs and to investigate the ability of these fish to spawn naturally. Fish collected as juveniles were held for a short time at the Sawtooth Fish Hatchery and later transferred to the Eagle Fish Hatchery for rearing. Eyed-eggs were transferred immediately to the Eagle Fish Hatchery where they were disinfected and reared by family groups. When fish from either collection method reached approximately 60 mm, they were PIT tagged and reared separately by brood year and source stream. Sixteen different groups were in culture at IDFG facilities in 1999. Hatchery spawning activities of captive-reared chinook salmon produced eyed-eggs for outplanting in streamside incubation chambers in the West Fork Yankee Fork Salmon River (N=2,297) and the East Fork Salmon River (N=1,038). Additionally, a number of these eggs were maintained at the Eagle Fish Hatchery to ensure adequate brood year 1999 representation from these systems, and produced 279 and 87 juveniles from the West Fork Yankee Fork and East Fork Salmon River, respectively. Eyed-eggs were not collected from the West Fork Yankee Fork due to low adult escapement. Brood year 1998 juveniles were collected from the Lemhi River (N=191), West Fork Yankee Fork Salmon River (N=229), and East Fork Salmon River (N=185). Additionally, brood year 1999 eyed-eggs were collected from the Lemhi River (N=264) and East Fork Salmon River (N=143). Sixty-two and seven maturing adults were released into Bear Valley Creek (Lemhi River system) and the East Fork Salmon River, respectively, for spawning evaluation in 1999. Nine female carcasses from Bear Valley Creek were examined for egg retention, and of these five were spawned out, one was partially spawned, and three died before depositing eggs. However, much of the spawning related behavior observed involved female chinook salmon paired with male bull trout Salvelinus confluentus. Two female carcasses from the East Fork Salmon River were recovered and examined for egg retention. One of these had spawned and one had not.

  12. Non-destructive elemental quantification of polymer-embedded thin films using laboratory based X-ray techniques

    NASA Astrophysics Data System (ADS)

    Cordes, Nikolaus L.; Havrilla, George J.; Usov, Igor O.; Obrey, Kimberly A.; Patterson, Brian M.

    2014-11-01

    Thin coatings are important for a variety of industries including energy (e.g., solar cells, batteries), consumer electronics (e.g., LCD displays, computer chips), and medical devices (e.g., implants). These coatings are typically highly uniform layers with thicknesses ranging from a monolayer up to several micrometers. Characterizing these highly uniform coatings for their thickness, elemental composition, and uniformity are all paramount, but obtaining these measurements can be more difficult when the layers are subsurface and must be interrogated non-destructively. The coupling of confocal micro-X-ray fluorescence (confocal MXRF) and nano-scale X-ray computed tomography (nano-CT) together can make these measurements while meeting these sensitivity and resolution specifications necessary for characterizing thin films. Elemental composition, atomic percent, placement, and uniformity can be measured in three dimensions with this integrated approach. Confocal MXRF uses a pair of polycapillary optics to focus and collect X-rays from a material from a 3D spatially restricted confocal volume. Because of the spatial definition, individual layers (of differing composition) can be characterized based upon the elementally characteristic X-ray fluorescence collected for each element. Nano-scale X-ray computed tomography, in comparison, can image the layers at very high resolution (down to 50 nm) to precisely measure the embedded layer thickness. These two techniques must be used together if both the thickness and atomic density of a layer are unknown. This manuscript will demonstrate that it is possible to measure both the atomic percent of an embedded thin film layer and confirm its manufacturing quality. As a proof of principle, a 1.5 atomic percent, 2 μm-thick Ge layer embedded within polymer capsules, used for laser plasma experiments at the Omega Laser Facility and National Ignition Facility, are measured.

  13. Isolation rearing of rats alters release of 5-hydroxytryptamine and dopamine in the frontal cortex: an in vivo electrochemical study.

    PubMed

    Crespi, F; Wright, I K; Möbius, C

    1992-01-01

    The effects of rearing hooded Lister rats either in groups of seven or singly on 5-hydroxytryptamine (5-HT) and dopamine (DA) release in the frontal cortex were investigated using in vivo voltammetry together with Nafion coated carbon fibre micro-electrodes. The selective detection of basal extracellular levels of 5-HT with this technique (Peak B) was confirmed with parallel experiments using intracranial microdialysis to measure 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) levels in vivo. The DA voltammetric signal (Peak A) was observed in vivo only following pharmacological or electrical stimulation of DA release. Enhanced efflux of cortical DA and 5-HT in response to local application of KCl and that of 5-HT following parentelar fenfluramine were selectively detected by the association: differential pulse voltammetry (DPV)-Nafion coated microbiosensors, supporting the capability of this electrochemical method to selectively monitor release of these amine neurotransmitters in vivo and in situ. The locomotor behaviour data indicated that isolation rearing resulted in augmented locomotor activity in a novel environment. In addition, the in vivo voltammetric results showed that following KCl or fenfluramine treatment cortical 5-HT release is prolonged while that of DA is increased in rats reared in isolation when compared with socially reared rats. This imbalance between extracellular levels of DA and 5-HT recorded in the frontal cortex of rats exposed to isolated housing conditions may contribute to the behavioural differences reported between isolation and group reared rats. PMID:1375164

  14. Multiple orifice distribution system for placing green lacewing eggs into verticel larval rearing units.

    PubMed

    Woolfolk, S W; Smith, D B; Martin, R A; Sumrall, B H; Nordlund, D A; Smith, R A

    2007-04-01

    Green lacewings are widely used biological control agents for various insect pests. To meet the needs of growers, green lacewings are being mass-reared commercially around the world. A common salt shaker has been used regularly to distribute eggs into Verticel lacewing larval rearing units. This technique is time consuming and inefficient because the number of eggs distributed in each cell is inconsistent. The multiple orifice distribution (MOD) system described here greatly improved egg distribution efficiency by increasing the percentage of Verticel cells containing the desired one to four eggs per cell (i.e., 40.8 and 52.1% by using salt shaker method versus 61.9% by using the MOD system). This mechanical system significantly reduced the labor and time involved in the process and would cost under $3500. In addition, this new system could be modified for distribution of other insect eggs. PMID:17461048

  15. Hand-rearing, growth, and development of common loon (Gavia immer) chicks

    USGS Publications Warehouse

    Kenow, Kevin P.; Meier, Melissa S.; McColl, Laurie E.; Hines, Randy K.; Pichner, Jimmy; Johnson, Laura; Lyon, James E.; Scharold, Kellie Kroc; Meyer, Michael

    2014-01-01

    Common loon chicks were reared in captivity in association with studies to evaluate the effects of radiotransmitter implants and to assess the ecological risk of dietary methylmercury. Here we report on hatching and rearing methods used to successfully raise chicks to 105 days of age. We experienced a 91.5% hatch rate, and 89.6% of loon chicks survived to the end of the study at 105 days. Baseline information on observed rates of fish consumption, behavioral development, and growth patterns are provided. Husbandry techniques are provided that should prove valuable to wildlife rehabilitators caring for abandoned or injured loons, and biologists contemplating methods for restoring loons to areas within their former breeding range.

  16. Fin development in stream- and hatchery-reared Atlantic salmon

    USGS Publications Warehouse

    Pelis, R.M.; McCormick, S.D.

    2003-01-01

    To determine the effect of development and environment on fin growth, we measured fin lengths of juvenile Atlantic salmon (Salmo salar) from two hatcheries (August, October and April-May), stream-reared fish (July and October) stocked as fry into two tributaries, and smelts from the main stem of the Connecticut River (May). For stream-reared parr, there was a linear relationship between the dorsal, caudal and anal fins with fork length, while the pectoral, pelvic and adipose fins exhibited a curvilinear relationship with fork length. Parr from a high gradient stream had larger caudal fins than fish from a low gradient stream, but other fins did not differ. Regression lines for the fins of stream-reared smelts were all linear when fin length was regressed against fork length. Stream-reared parr had larger pectoral, pelvic and anal fins than smolts of similar size while dorsal and caudal fin lengths did not differ. Regression equations formulated using the fins of stream-reared parr were used to calculate the percent difference (100 x observed fin length/expected) in fin lengths between stream- and hatchery-reared parr. The pelvic, adipose, caudal and anal fins of hatchery-reared parr showed no signs of degeneration by the first sampling period 7 months after hatching, whereas degeneration in the pectoral (13-20%) and dorsal (15-18%) fins was evident at this time. By the end of the study, degeneration was present in every fin except the adipose, with the pectoral (35-65%) and dorsal (32-58%) fins exhibiting the greatest amount of fin loss. All fins of hatchery-reared parr became shorter with time. There were minor differences in fin degeneration among parr from the two hatcheries, but the overall pattern of decreasing fin size was similar, indicating a common cause of fin degeneration. Comparison of stream- and hatchery-reared fish is a valuable means of determining the impact of captive environments on fin growth.

  17. Sexual performance of mass reared and wild Mediterranean fruit flies (Diptera: Tephritidae) from various origins of the Madeira Islands

    SciTech Connect

    Pereira, R.; Silva, N.; Quintal, C.; Abreu, R.; Andrade, J.; Dantas, L.

    2007-03-15

    The success of Mediterranean fruit fly (medfly) Ceratitis capitata (Wiedemann) control programs integrating the sterile insect technique (SIT) is based on the capacity of released the sterile males to compete in the field for mates. The Islands of Madeira are composed of 2 populated islands (Madeira and Porto Santo) where the medfly is present. To evaluate the compatibility and sexual performance of sterile flies we conducted a series of field cage tests. At same time, the process of laboratory domestication was evaluated. 3 wild populations, one semi-wild strain, and 1 mass reared strain were evaluated: the wild populations of (1) Madeira Island (north coast), (2) Madeira Island (south coast), and (3) Porto Santo Island; (4) the semi-wild population after 7 to 10 generations of domestication in the laboratory (respectively, for first and second experiment); and (5) the genetic sexing strain in use at Madeira medfly facility (VIENNA 7mix2000). Field cage experiments showed that populations of all origins are mostly compatible. There were no significant differences among wild populations in sexual competitiveness. Semi-wild and mass-reared males performed significantly poorer in both experiments than wild males in achieving matings with wild females. The study indicates that there is no significant isolation among strains tested, although mating performance is reduced in mass-reared and semi-wild flies after 7 to 10 generations in the laboratory. (author) [Spanish] El exito de los programas de control de la mosca mediterranea de la fruta (Ceratitis capitata (Wiedemann) que integran la tecnica del insecto esteril (TIE) esta basado en la capacidad de machos esteriles para competir en el campo por sus parejas. Las Islas de Madeira consisten de 2 islas pobladas (Madeira y Porto Santo) donde la mosca mediterranea de la fruta esta presente. Para evaluar la compatibilidad y el funcionamiento sexual de moscas esteriles nosotros realizamos una serie de pruebas de jaula en el campo. Al mismo tiempo, el proceso de la domesticacion en el laboratorio fue evaluado. Tres poblaciones naturales, una poblacion semi-natural y una poblacion criada en masa fueron evaluadas: las poblaciones natural de (1) Isla de Madeira (costa norte), (2) Isla de Madeira (costa sur) y (3) Isla de Porto Santo; (4) una poblacion semi-natural despues de 7 a 10 generaciones de domesticacion en el laboratorio (respectivamente, para el primero y segundo experimento); y (5) la raza para separar sexos geneticamente que es usada en el laboratorio de la mosca mediterranea de Madeira (VIENNA 7mix2000). Los experimentos usando jaulas en el campo mostraron que las poblaciones de diferentes origines fueron en su mayor parte compatibles. No hubo diferencias significativas en la capacidad para competir sexualmente entre las poblaciones naturales. Los machos semi-naturales y los machos criados en masa mostraron un desempeno significativamente bajo en ambos experimentos que los machos naturales en el logro de copula con las hembras naturales. Este estudio indica que no hay un aislamiento significativo entre las razas probadas, aunque el desempeno en el apareamiento fue reducido en las moscas criadas en masa y semi-naturales despues de 7 a 10 generaciones en el laboratorio. (author)

  18. Cooperative fish-rearing programs in Hanford Site excess facilities

    SciTech Connect

    Herborn, D.I.; Anderson, B.N.

    1994-05-01

    In, 1993, two successful fish-rearing pilot projects were conducted in Hanford Site 100 K Area water treatment pools (K Pools) that are excess to the US Department of Energy needs. Beginning this spring, two larger cooperative fish programs will be undertaken in the K Pools. One program will involve the Yakama Indian Nation, which will rear, acclimate, and release 500,000 fall chinook salmon. The other program involves the Washington Department of Fish and Wildlife, which will rear warm-water specie (walleye and channel catfish) for planting in state lakes. Renewed economic vitality is the goal expected from these and follow-on fish programs.

  19. [Laboratory tests on cancer].

    PubMed

    Kondo, S

    1990-01-01

    Extensive research on "Cancer" have been undertaken using inovative techniques. However, the knowledge and techniques gained from this research has not fully been introduced into clinical laboratories, except for analysis of tumor markers in the blood or in the cells and some oncogenes. In this symposium, we show some possibilities in transferring the new knowledge and techniques from research laboratories to clinical laboratories. These lectures will inspire us to introduce more tests on "Cancer" into the laboratories. PMID:2308220

  20. Methods for rearing Syngaster lepidus and Jarra phoracantha (Hymenoptera: Braconidae), larval parasitoids of the phloem-colonizing longhorned beetles Phoracantha semipunctata and P. recurva (Coleoptera: Cerambycidae).

    PubMed

    Millar, J G; Millar, J G; Paine, T D; Campbell, C D; Hanks, L M

    2002-04-01

    Two species of cerambycid beetles that attack eucalypts, Phoracantha semipunctata (Fabricius) and P. recurva Newman, have been accidentally introduced from Australia into most regions of the world in which their hosts have been planted. The beetles cause extensive mortality in plantations and landscape plantings of the trees. Management programmes have focused on integration of silvicultural practices, host plant resistance and biological control. To rear and release natural enemies of the larval stages of the beetles in California, mass rearing protocols for continuous production of two species of parasitoids have been developed. The methods described represent the first long-term and large-scale techniques for mass rearing parasitoids of any wood-boring cerambycid species. In addition to providing large numbers of parasitoids for releases, the mass rearing effort has also provided large numbers of parasitoids for fundamental studies of their biology and behaviour. PMID:12020372

  1. Locally contacted rear surface passivated solar cells by inkjet printing

    NASA Astrophysics Data System (ADS)

    Phiwpha, N.; Fangsuwannarak, T.; Sopitpan, S.

    2014-06-01

    Inkjet printing of photoresist material may provide a new route for low-cost fabrication of patterned oxide passivation layer of solar cells that require fine patterning and simple process. However, printing by liquid-based, environmentally friendly ink and printing device required development efforts aimed at achieving a fine patterning and long used inkjet nozzles under corrosive influence. This work was demonstrated a concept for grooved silicon oxide patterning for rear localized contact of p-n junction solar cells by chemical etching after photoresist patterning obtained. This article reviews the silicon dioxide fabrication on p-Si substrate from sol-gel technique for oxide passivation layer of solar cells. The aluminium was deposited on the patterned oxide layer and then heated at its Al-Si eutectic temperature. Finally, an aluminium-induced solid-phase epitaxial growth of p+ forming into the openings of the oxide passivation layer was presented. The sheet resistance of n-emitter layer, carrier life-time and surface recombination velocity values are investigated. Photoconductive measurements were performed on the prepared samples after each thermal process to measure the effective lifetime of the minority carriers. Carrier lifetime up to 60 microseconds has been measured on c-Si wafer passivated by the opened SiO2 layer. It was shown that the patterned SiO2 passivation has obtained high passivation quality making by the proposed inkjet printing method.

  2. 6. VIEW SHOWING NORTHEAST END OF WHARF REAR FROM LANDSLIDE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. VIEW SHOWING NORTHEAST END OF WHARF REAR FROM LANDSLIDE - U.S. Naval Base, Pearl Harbor, Berthing Wharf S378, Beckoning Point, Southeast of Cowpens Street, Ford Island, Pearl City, Honolulu County, HI

  3. 4. WASHBURN POINT VISTA AREA. HALF DOME AT CENTER REAR. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. WASHBURN POINT VISTA AREA. HALF DOME AT CENTER REAR. LOOKING NE. GIS: N-37 43 13.7 / W-119 34 23.0 - Glacier Point Road, Between Chinquapin Flat & Glacier Point, Yosemite Village, Mariposa County, CA

  4. OBLIQUE VIEW, REAR ELEVATION, LOOKING SOUTHSOUTHWEST Mountain Home Air ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    OBLIQUE VIEW, REAR ELEVATION, LOOKING SOUTH-SOUTHWEST - Mountain Home Air Force Base 1958 Senior Officers' Housing, General's Residence, Rabeni Street (originally Ivy Street), Mountain Home, Elmore County, ID

  5. 1. Northwest end and southwest rear. View to east. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Northwest end and southwest rear. View to east. - Offutt Air Force Base, Looking Glass Airborne Command Post, Squadron Operations Building, At southwestern-most boundary of Project Looking Glass Historic District, Bellevue, Sarpy County, NE

  6. 11. Interior view of communications compartment. View toward rear of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Interior view of communications compartment. View toward rear of aircraft. - Offutt Air Force Base, Looking Glass Airborne Command Post, Looking Glass Aircraft, On Operational Apron covering northeast half of Project Looking Glass Historic District, Bellevue, Sarpy County, NE

  7. 3. General view showing rear of looking glass aircraft. View ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. General view showing rear of looking glass aircraft. View to north. - Offutt Air Force Base, Looking Glass Airborne Command Post, Looking Glass Aircraft, On Operational Apron covering northeast half of Project Looking Glass Historic District, Bellevue, Sarpy County, NE

  8. 9. Interior view of electronics compartment. View toward rear of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Interior view of electronics compartment. View toward rear of aircraft. - Offutt Air Force Base, Looking Glass Airborne Command Post, Looking Glass Aircraft, On Operational Apron covering northeast half of Project Looking Glass Historic District, Bellevue, Sarpy County, NE

  9. 8. Steve Dunwell, Photographer, March 1979. REAR VIEW SHOWING CONNECTING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Steve Dunwell, Photographer, March 1979. REAR VIEW SHOWING CONNECTING ROD, CRANK, BEARING AND FLYWHEEL OF 1910 WM. A. HARRIS ENGINE. - Merchants Cold Storage Warehouse, 160 Kingsley Avenue, Providence, Providence County, RI

  10. West (side) and south (rear) elevations of building. View to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    West (side) and south (rear) elevations of building. View to north. - Plattsburgh Air Force Base, Power Check Pad with Suppressor, Near Idaho at Alabama Avenue, on flightline Apron, Plattsburgh, Clinton County, NY

  11. South (rear) and west (side) elevations of building. View to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South (rear) and west (side) elevations of building. View to north. - Plattsburgh Air Force Base, Power Check Pad with Suppressor, Near Idaho at Alabama Avenue, on flightline Apron, Plattsburgh, Clinton County, NY

  12. South (rear) elevation of building, with exhaust shaft in right ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South (rear) elevation of building, with exhaust shaft in right foreground. View to north. - Plattsburgh Air Force Base, Power Check Pad with Suppressor, Near Idaho at Alabama Avenue, on flightline Apron, Plattsburgh, Clinton County, NY

  13. OBLIQUE VIEW OF REAR ELEVATION OF MARINE BARRACKS, LOOKING WEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    OBLIQUE VIEW OF REAR ELEVATION OF MARINE BARRACKS, LOOKING WEST NORTHWEST. - Naval Computer & Telecommunications Area Master Station, Eastern Pacific, Radio Transmitter Facility Lualualei, Marine Barracks, Intersection of Tower Drive & Morse Street, Makaha, Honolulu County, HI

  14. Detail, rear door types, building 242, oblique view to southwest, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail, rear door types, building 242, oblique view to southwest, 90 mm lens. - Travis Air Force Base, Nuclear Weapons Assembly Building, W Street, Armed Forces Special Weapons Project Q Area, Fairfield, Solano County, CA

  15. 9. NORTH (REAR) SIDE, ENTRY AND STAIRHALL DORMER. This dormer ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. NORTH (REAR) SIDE, ENTRY AND STAIRHALL DORMER. This dormer not original with house; note Flemish bond brickwork v.s. English bond brickwork of front and side elevations. - Mulberry, Cooper River, West Branch, Moncks Corner, Berkeley County, SC

  16. J SERIES MAGAZINE. J 107 NORTH END AND REAR (EAST). ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    J SERIES MAGAZINE. J 107 NORTH END AND REAR (EAST). J 106-103 IN BACKGROUND. - Naval Magazine Lualualei, Headquarters Branch, Inert Storehouse Type, Twelfth Street between Kwajulein & New Mexico Streets, Pearl City, Honolulu County, HI

  17. 4. NORTH REAR, CONTROL TOWER AND CONTROL HOUSE, SHOWING INTAKE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. NORTH REAR, CONTROL TOWER AND CONTROL HOUSE, SHOWING INTAKE STRUCTURE TRASH RACKS BELOW. - Prado Dam, Outlet Works, Santa Ana River near junction of State Highways 71 & 91, Corona, Riverside County, CA

  18. 48. DETAIL OF REAR OF DEMULTIPLEX PANEL 5 SHOWING COMPONENTS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    48. DETAIL OF REAR OF DEMULTIPLEX PANEL 5 SHOWING COMPONENTS OF VACUUM-TUBE OSCILLOSCOPE - Vandenberg Air Force Base, Space Launch Complex 3, Launch Operations Building, Napa & Alden Roads, Lompoc, Santa Barbara County, CA

  19. View of corredera supports and rear (southwest) of house, including ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of corredera supports and rear (southwest) of house, including attachment of rails to la casa, view towards the east - Finca Thillett, Casa, Highway 139, Kilometer 11.6, Maraguez, Ponce Municipio, PR

  20. 16. EXTERIOR, GENERAL VIEW OF WEST (REAR) ELEVATION FROM THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. EXTERIOR, GENERAL VIEW OF WEST (REAR) ELEVATION FROM THE NORTHWEST - Mark Twain House, 351 Farmington Avenue (corrected from original address of 531 Farmington Avenue), Hartford, Hartford County, CT

  1. 15. EXTERIOR, GENERAL VIEW OF WEST (REAR) ELEVATION AND PARTIAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. EXTERIOR, GENERAL VIEW OF WEST (REAR) ELEVATION AND PARTIAL NORTH ELEVATION FROM THE NORTHWEST - Mark Twain House, 351 Farmington Avenue (corrected from original address of 531 Farmington Avenue), Hartford, Hartford County, CT

  2. 11. VIEW NORTH OF REAR OF TOW VEHICLE. NOTE CURRENT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. VIEW NORTH OF REAR OF TOW VEHICLE. NOTE CURRENT COLLECTORS AT TOP LEFT AND OVERHEAD TROLLEY WIRES FOR POWER TRANSMISSION TO DRIVE MOTORS. - NASA Langley Research Center, Seaplane Towing Channel, 108 Andrews Street, Hampton, Hampton, VA

  3. 54. POWDER MAGAZINE, VENTILATION PASSAGE ALONG REAR. NOTE STONE RUBBLE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    54. POWDER MAGAZINE, VENTILATION PASSAGE ALONG REAR. NOTE STONE RUBBLE CONSTRUCTION TO LEFT (SOUTHWEST); ENTRANCE TO A MAGAZINE TO THE RIGHT. VIEW IS NORTHWEST TO SOUTHEAST. - Fort Monroe, Fortress, Hampton, Hampton, VA

  4. VIEW OF ONESTAMP MILL WITH RANCH HOUSE AT REAR (See ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF ONE-STAMP MILL WITH RANCH HOUSE AT REAR (See HABS No. CA-2347, DESERT QUEEN RANCH, for further documentation) - Desert Queen Ranch, One Stamp Gold Mill, Twentynine Palms, San Bernardino County, CA

  5. 4. REAR VIEW OF BUILDING NO. 93 FACING SOUTHWEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. REAR VIEW OF BUILDING NO. 93 FACING SOUTHWEST - U.S. Naval Base, Pearl Harbor, Alcohol Rehabilitation Center, Nimitz Spur between Sixth Street & Naval Station North Road, Pearl City, Honolulu County, HI

  6. 19. INTERIOR OF NORTHEAST REAR BEDROOM SHOWING ALUMINUMFRAME SLIDING GLASS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. INTERIOR OF NORTHEAST REAR BEDROOM SHOWING ALUMINUM-FRAME SLIDING GLASS WINDOWS. VIEW TO NORTHEAST. - Bishop Creek Hydroelectric System, Plant 4, Worker Cottage, Bishop Creek, Bishop, Inyo County, CA

  7. 21. INTERIOR OF SOUTHEAST REAR BEDROOM SHOWING ALUMINUMFRAME SLIDING GLASS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. INTERIOR OF SOUTHEAST REAR BEDROOM SHOWING ALUMINUM-FRAME SLIDING GLASS WINDOWS. VIEW TO SOUTHEAST. - Bishop Creek Hydroelectric System, Plant 4, Worker Cottage, Bishop Creek, Bishop, Inyo County, CA

  8. 5. Oblique view of rear (southeast) and right side (southwest) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Oblique view of rear (southeast) and right side (southwest) elevations, looking north. - Downtown Short Pump Grocery, West Broad Street (State Route 250) & Three Chopt Road, Short Pump, Henrico County, VA

  9. 6. East rear, cattle auction arena below in background, Omaha ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. East rear, cattle auction arena below in background, Omaha livestock market offices above. View to west. - South Omaha Union Stock Yards, Stock Yards Autopark, 2900 "O" Plaza, Omaha, Douglas County, NE

  10. 180 degree view of two story unit illustrating rear elevation. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    180 degree view of two story unit illustrating rear elevation. Building 37, view facing northeast - Harbor Hills Housing Project, Two Story Townhouse Type, 26607 Western Avenue, Lomita, Los Angeles County, CA

  11. 180 degree view of Building 20, illustrating rear elevation of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    180 degree view of Building 20, illustrating rear elevation of two story walk-up flat type unit. View facing northwest - Harbor Hills Housing Project, Two Story Walk-Up Type, 26607 Western Avenue, Lomita, Los Angeles County, CA

  12. Detail of secondstory balcony at rear of threestory section of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of second-story balcony at rear of three-story section of Type A residential building. View to east. - Lincoln Park Homes, Type A Residential Building, West Colfax Avenue & Marispoa Street, Denver, Denver County, CO

  13. 28. Rear lot of the Adelman Block. The collapsed truss ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    28. Rear lot of the Adelman Block. The collapsed truss roof (ca. 1932) originally sheltered an automobile sales garage - Lockport Historic District, Bounded by Eighth, Hamilton & Eleventh Streets & Illinois & Michigan Canal, Lockport, Will County, IL

  14. 2. REAR AND SOUTH SIDE VIEW OF BUILDING NO. 1 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. REAR AND SOUTH SIDE VIEW OF BUILDING NO. 1 FACING NORTHEAST. - U.S. Naval Base, Pearl Harbor, Ordinance Operations Building, West Loch, First Street near Whiskey Wharves W1 & W2, Pearl City, Honolulu County, HI

  15. 6. Interior, rear offices: operations assistant office looking north toward ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Interior, rear offices: operations assistant office looking north toward security operations officer's office. - Ellsworth Air Force Base, Rushmore Air Force Station, Security Central Control Building, Quesada Drive, Blackhawk, Meade County, SD

  16. 6. OUTER BLAST DOOR, WEST REAR. Edwards Air Force ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. OUTER BLAST DOOR, WEST REAR. - Edwards Air Force Base, South Base Sled Track, Firing & Control Blockhouse for 10,000-foot Track, South of Sled Track at midpoint of 20,000-foot track, Lancaster, Los Angeles County, CA

  17. 5. NORTH SIDE AND WEST REAR. Edwards Air Force ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. NORTH SIDE AND WEST REAR. - Edwards Air Force Base, South Base Sled Track, Firing & Control Blockhouse for 10,000-foot Track, South of Sled Track at midpoint of 20,000-foot track, Lancaster, Los Angeles County, CA

  18. 4. CLOSEUP OF REAR STEPPED PARAPET GABLE SHOWING TOP CURVILINEAR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. CLOSE-UP OF REAR STEPPED PARAPET GABLE SHOWING TOP CURVILINEAR GABLE AND ROUGH STUCCO FINISH. VIEW TO THE SOUTHEAST. - Bishop Creek Hydroelectric System, Control Station, Operations Building No. 1, Bishop Creek, Bishop, Inyo County, CA

  19. Perspective circular rearing ponds on north side of hatchery. View ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Perspective circular rearing ponds on north side of hatchery. View to the west, northwest. Includes view of ditch and step up to hatchery building on left side of image. - Prairie Creek Fish Hatchery, Hwy. 101, Orick, Humboldt County, CA

  20. SOUTHWEST REAR, SHOWING CLOSED ENTRY HATCH, BUILDING 1934. Looking north ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SOUTHWEST REAR, SHOWING CLOSED ENTRY HATCH, BUILDING 1934. Looking north - Edwards Air Force Base, X-15 Engine Test Complex, Observation Bunker Types, Rogers Dry Lake, east of runway between North Base & South Base, Boron, Kern County, CA

  1. 98. 181721 WEST MUHAMMAD ALI BOULEVARD, PART OF SOUTH REAR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    98. 1817-21 WEST MUHAMMAD ALI BOULEVARD, PART OF SOUTH REAR ON LEFT, TOWARD SOUTHWEST AND SOUTH NINETEENTH STREET - Russell Neighborhood, Bounded by Congress & Esquire Alley, Fifteenth & Twenty-first Streets, Louisville, Jefferson County, KY

  2. Environmental assessment, K Pool fish rearing, Hanford Site, Richland, Washington

    SciTech Connect

    1996-12-01

    The US Department of Energy (DOE) has a need to respond to a request to lease facilities at the Hanford Site 100-KE and 100-KW filter plant pools (K Pools) for fish rearing activities. These fish rearing activities would be: (1) business ventures with public and private funds and (2) long-term enhancement and supplementation programs for game fish populations in the Columbia River Basin. The proposed action is to enter into a use permit or lease agreement with the YIN or other parties who would rear fish in the 100-K Area Pools. The proposed action would include necessary piping, pump, and electrical upgrades of the facility; cleaning and preparation of the pools; water withdrawal from the Columbia River, and any necessary water or wastewater treatment; and introduction, rearing and release of fish. Future commercial operations may be included.

  3. Contextual view including south (rear) of building 925, exercise in ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Contextual view including south (rear) of building 925, exercise in foreground, and modern buildings in background. Facing northwest. - Travis Air Force Base, Building No. 925, W Street, Fairfield, Solano County, CA

  4. 3. REAR VIEW, AUTOMATIC BLOCK SIGNAL, EASTBOUND ON CATENARY BRIDGE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. REAR VIEW, AUTOMATIC BLOCK SIGNAL, EASTBOUND ON CATENARY BRIDGE 486 - New York, New Haven & Hartford Railroad, Automatic Signalization System, Long Island Sound shoreline between Stamford & New Haven, Stamford, Fairfield County, CT

  5. 20. Mill River and rear of the 1860 armory building, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. Mill River and rear of the 1860 armory building, c. 1930. Photocopied from a print of a film negative, NHCHSL. View from the south. - Eli Whitney Armory, West of Whitney Avenue, Armory Street Vicinity, Hamden, New Haven County, CT

  6. 1. VIEW NORTHWEST, EAST (REAR) ELEVATION OF 305 CHURCH STREET ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW NORTHWEST, EAST (REAR) ELEVATION OF 305 CHURCH STREET WITH 313 and 317 CHURCH STREET IN BACKGROUND - Putnam Manufacturing Company Workers' Houses, 305 Church Street (House), Putnam, Windham County, CT

  7. 7. VIEW OF CONNECTING WALL BETWEEN FRONT AND REAR ROOMS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. VIEW OF CONNECTING WALL BETWEEN FRONT AND REAR ROOMS OF SECOND FLOOR, SHOWING SIMPLE GREEK REVIVAL MANTLE. LOOKING WEST; TAKEN FROM FRONT ROOM. - Manlius Thomas House, 125 North Mulberry Street, Georgetown, Scott County, KY

  8. East rear, northern part, looking west across the lawn near ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    East rear, northern part, looking west across the lawn near the Greek theater. - San Bernardino Valley College, Classics Building, 701 South Mount Vernon Avenue, San Bernardino, San Bernardino County, CA

  9. NORTH (REAR) AND EAST (SIDE) ELEVATIONS OF BUILDING. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    NORTH (REAR) AND EAST (SIDE) ELEVATIONS OF BUILDING. VIEW TO SOUTHWEST. - Plattsburgh Air Force Base, Solid Waste Disposal Facility, Off Connecticut Road, west of Idaho Avenue, Plattsburgh, Clinton County, NY

  10. 2. EQUIPMENT STORAGE SHED, EAST REAR AND SOUTH SIDE LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. EQUIPMENT STORAGE SHED, EAST REAR AND SOUTH SIDE LOOKING NORTHWEST - Union Ranger District Compound, Equipment Storage Shed, Fronting State Highway 203, at West edge of Union, Union, Union County, OR

  11. 9. DETAIL OF REAR ENTRANCE ON EAST WALL OF NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. DETAIL OF REAR ENTRANCE ON EAST WALL OF NORTH WING OF BUILDING. VIEW TO NORTHWEST. - Fort David A. Russell, Red Cross Building, Third Street between Randall Avenue & Tenth Cavalry Avenue, Cheyenne, Laramie County, WY

  12. 3. COMPARATIVE PHOTO SHOWING DIFFERENT REAR ENTRIES ON TWO BUILDINGS, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. COMPARATIVE PHOTO SHOWING DIFFERENT REAR ENTRIES ON TWO BUILDINGS, BUILDING MT-76-A IN FOREGROUND - Fort Keogh, Livestock & Range Research Station, 3 miles west of Miles City on U.S. Highway 10, Miles City, Custer County, MT

  13. CONTEXT VIEW OF REAR OF HULETTS IN FRONT OF MODERN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CONTEXT VIEW OF REAR OF HULETTS IN FRONT OF MODERN SELF-UNLOADING BOOM. LOOKING NORTH. - Pennsylvania Railway Ore Dock, Lake Erie at Whiskey Island, approximately 1.5 miles west of Public Square, Cleveland, Cuyahoga County, OH

  14. 14. VEHICLE STORAGE BUILDING NORTHWEST SIDE AND NORTHEAST REAR. VIEW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. VEHICLE STORAGE BUILDING NORTHWEST SIDE AND NORTHEAST REAR. VIEW TO SOUTHEAST. - Minuteman III ICBM Launch Control Facility November-1, 1.5 miles North of New Raymer & State Highway 14, New Raymer, Weld County, CO

  15. 4. LAUNCH CONTROL SUPPORT BUILDING NORTH SIDE AND EAST REAR. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. LAUNCH CONTROL SUPPORT BUILDING NORTH SIDE AND EAST REAR. VIEW TO SOUTHWEST. - Minuteman III ICBM Launch Control Facility November-1, 1.5 miles North of New Raymer & State Highway 14, New Raymer, Weld County, CO

  16. View of rear of Childs Powerhosue. Rockwork on east end ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of rear of Childs Powerhosue. Rockwork on east end was recently replaced following a flood. Looking south-southwest - Childs-Irving Hydroelectric Project, Childs System, Childs Powerhouse, Forest Service Road 708/502, Camp Verde, Yavapai County, AZ

  17. View of east and south (rear) walls, water wheels and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of east and south (rear) walls, water wheels and generators, interior of Childs Powerhouse. Looking southeast - Childs-Irving Hydroelectric Project, Childs System, Childs Powerhouse, Forest Service Road 708/502, Camp Verde, Yavapai County, AZ

  18. Looking West From rear (East) End of Office Building Including ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Looking West From rear (East) End of Office Building Including Recycle Storage Area, Loading Docks, and Decontamination Zone - Hematite Fuel Fabrication Facility, Office, 3300 State Road P, Festus, Jefferson County, MO

  19. 2. BUILDING 0521, SOUTH REAR AND EAST SIDE. Looking to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. BUILDING 0521, SOUTH REAR AND EAST SIDE. Looking to northwest from access road. - Edwards Air Force Base, South Base Sled Track, Earth Covered Bunker Types, North of Sled Track, Lancaster, Los Angeles County, CA

  20. 75. SACRED HEART SCHOOL, 1324 ELLIS STREET SOUTH (REAR ELEVATION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    75. SACRED HEART SCHOOL, 1324 ELLIS STREET SOUTH (REAR ELEVATION FROM GREENE STREET 56/61A - Greene Street Historic District, Greene Street, Gordon Highway to Augusta Canal Bridge, Augusta, Richmond County, GA

  1. View east, showing Northwest Wing (Wing 5) and rear elevations ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View east, showing Northwest Wing (Wing 5) and rear elevations of facade and tis flaking wings (Wings 1 and 2) - Hospital for Sick Children, 1731 Bunker Hill Road, Northeast, Washington, District of Columbia, DC

  2. 4. Office and warehouse building. North side and rear (west) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Office and warehouse building. North side and rear (west) elevation. View to south. - Conrad Refining Company Oil Refinery, Office & Warehouse Building, 60 feet northwest of centerline at Montana Secondary Highway 219, Conrad, Pondera County, MT

  3. 6. Office and warehouse building. Rear elevation and south side. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Office and warehouse building. Rear elevation and south side. View to east. - Conrad Refining Company Oil Refinery, Office & Warehouse Building, 60 feet northwest of centerline at Montana Secondary Highway 219, Conrad, Pondera County, MT

  4. 5. Office and warehouse building. Rear elevation. View to southeast. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Office and warehouse building. Rear elevation. View to southeast. - Conrad Refining Company Oil Refinery, Office & Warehouse Building, 60 feet northwest of centerline at Montana Secondary Highway 219, Conrad, Pondera County, MT

  5. BARBEQUE PIT AND PLAYHOUSE IN (REAR) YARD, LOOKING SOUTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    BARBEQUE PIT AND PLAYHOUSE IN (REAR) YARD, LOOKING SOUTH - Irvine Ranch Agricultural Headquarters, Carillo Tenant House, Southwest of Intersection of San Diego & Santa Ana Freeways, Irvine, Orange County, CA

  6. Everett Weinreb, Photographer, April 1989 GARAGE BEHIND HOUSE, WEST (REAR), ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Everett Weinreb, Photographer, April 1989 GARAGE BEHIND HOUSE, WEST (REAR), LOOKING NORTHEAST - Irvine Ranch Agricultural Headquarters, Boyd Tenant House, Southeast of Intersection of San Diego & Santa Ana Freeways, Irvine, Orange County, CA

  7. CHICKEN COOP BEHIND FENCED YARD AND (REAR) OF BARBEQUE PIT, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CHICKEN COOP BEHIND FENCED YARD AND (REAR) OF BARBEQUE PIT, LOOKING NORTH - Irvine Ranch Agricultural Headquarters, Carillo Tenant House, Southwest of Intersection of San Diego & Santa Ana Freeways, Irvine, Orange County, CA

  8. 8. WEST REAR, DETAIL SHOWING SECOND FLOOR ENTRANCE AND WINDOW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. WEST REAR, DETAIL SHOWING SECOND FLOOR ENTRANCE AND WINDOW TREATMENT. See CA-174-B-22 FOR INTERIOR OF SECOND FLOOR LAB AT CENTER. - Hughes Aircraft Company, Engineering-Administration Building, 6775 Centinela Avenue, Los Angeles, Los Angeles County, CA

  9. 2. EAST REAR AND NORTH SIDE OF FIRE STATION. VIEW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. EAST REAR AND NORTH SIDE OF FIRE STATION. VIEW TO SOUTHWEST. - Rocky Mountain Arsenal, Fire Station, 80 feet North of December Seventh Avenue; 120 feet East of D Street, Commerce City, Adams County, CO

  10. 6. EXTERIOR OF REAR (EAST END) AND NORTH SIDE SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. EXTERIOR OF REAR (EAST END) AND NORTH SIDE SHOWING ASBESTOS SIDING, BACKYARD LAWN, AND CLOTHESLINE. VIEW TO SOUTHWEST. - Bishop Creek Hydroelectric System, Plant 4, Worker Cottage, Bishop Creek, Bishop, Inyo County, CA

  11. 15. View of Ford Mansion's living room looking towards rear ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. View of Ford Mansion's living room looking towards rear of house; scale is in back of room - Richmond Hill Plantation, Ford Mansion, East of Richmond Hill on Ford Neck Road, Richmond Hill, Bryan County, GA

  12. 5. CLUBHOUSE. REAR (NORTHWEST) SIDE. VIEW TO SOUTHSOUTHWEST. Rainbow ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. CLUBHOUSE. REAR (NORTHWEST) SIDE. VIEW TO SOUTH-SOUTHWEST. - Rainbow Hydroelectric Facility, Clubhouse, On north bank of Missouri River 2 miles Northeast of Great Falls, & end of Rainbow Dam Road, Great Falls, Cascade County, MT

  13. 63. DETAIL VIEW OF REAR DOOR, BUTTRESSES, AND PARAPETED GABLE, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    63. DETAIL VIEW OF REAR DOOR, BUTTRESSES, AND PARAPETED GABLE, NEPTUNE'S LOCKER, SOUTHEAST SIDE, LOOKING NORTHWEST - Huntington Beach Municipal Pier, Pacific Coast Highway at Main Street, Huntington Beach, Orange County, CA

  14. 68. VIEW SHOWING SOUTHWEST REAR OF NEPTUNE'S LOCK (LEFT) AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    68. VIEW SHOWING SOUTHWEST REAR OF NEPTUNE'S LOCK (LEFT) AND CAPTAIN'S GALLEY (RIGHT), LOOKING NORTHEAST - Huntington Beach Municipal Pier, Pacific Coast Highway at Main Street, Huntington Beach, Orange County, CA

  15. VIEW OF THE REAR OF WATERSIDE MALL Southwest Washington, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF THE REAR OF WATERSIDE MALL - Southwest Washington, Urban Renewal Area, Bounded by Independence Avenue, Washington Avenue, South Capitol Street, Canal Street, P Street, Maine Avenue & Washington Channel, Fourteenth Street, D Street, & Twelfth Street, Washington, District of Columbia, DC

  16. 19. Historic American Buildings Survey L. C. Durette, Photographer REAR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. Historic American Buildings Survey L. C. Durette, Photographer REAR STAIRS 1st. & 2nd. FLOOR SHOWING POST OF CHIMNEY GIRT - Doe Garrison, Lamprey River & Great Bay, Newmarket, Rockingham County, NH

  17. 20. Historic American Buildings Survey L. C. Durette, Photographer REAR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. Historic American Buildings Survey L. C. Durette, Photographer REAR STAIRS 1st. TO 2nd. FLOOR SHOWING POST OF CHIMNEY GIRT & OVERHANG - Doe Garrison, Lamprey River & Great Bay, Newmarket, Rockingham County, NH

  18. Detail of southwest corner showing rear double door entry, bracketed ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of southwest corner showing rear double door entry, bracketed window awnings, and decorative parapet coping, facing northeast. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  19. 2. WATER TREATMENT PUMPING AND STORAGE BUILDING, REAR AND RIGHT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. WATER TREATMENT PUMPING AND STORAGE BUILDING, REAR AND RIGHT SIDES, LOOKING SOUTHWEST. - NIKE Missile Base SL-40, Water Treatment & Storage Building, Southern portion of launch area, southeast of Ready Building, Hecker, Monroe County, IL

  20. Perspective view of north rear and west side, also showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Perspective view of north rear and west side, also showing north hall at far left. - San Bernardino Valley College, Life Science Building, 701 South Mount Vernon Avenue, San Bernardino, San Bernardino County, CA

  1. North rear, east part. Ramp leads to basement utility rooms ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    North rear, east part. Ramp leads to basement utility rooms and specimen preparation rooms. - San Bernardino Valley College, Life Science Building, 701 South Mount Vernon Avenue, San Bernardino, San Bernardino County, CA

  2. North rear, west part. Administration building is visible at far ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    North rear, west part. Administration building is visible at far right. - San Bernardino Valley College, Life Science Building, 701 South Mount Vernon Avenue, San Bernardino, San Bernardino County, CA

  3. Life sciences building, north rear, also showing north hall to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Life sciences building, north rear, also showing north hall to the right, and the library in the center distance. - San Bernardino Valley College, 701 South Mount Vernon Avenue, San Bernardino, San Bernardino County, CA

  4. FACILITY 82. REAR AND SIDE OBLIQUE. VIEW FACING NORTH. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    FACILITY 82. REAR AND SIDE OBLIQUE. VIEW FACING NORTH. - U.S. Naval Base, Pearl Harbor, Naval Housing Area Hale Alii, Shared Carport Type with Storerooms, End of driveways off Hale Alii Avenue, Pearl City, Honolulu County, HI

  5. General view of Buildings No. 56, 61, and 79 (rear ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General view of Buildings No. 56, 61, and 79 (rear facades and shared garage) from west. - National Home for Disabled Volunteer Soldiers, Northwestern Branch, Quarters, 5000 West National Avenue, Milwaukee, Milwaukee County, WI

  6. NORTHEAST (SIDE) AND NORTHWEST (REAR) ELEVATIONS OF BUILDING. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    NORTHEAST (SIDE) AND NORTHWEST (REAR) ELEVATIONS OF BUILDING. VIEW TO SOUTH - Plattsburgh Air Force Base, Industrial Wastewater Treatment & Disposal Facility, Off LeMay Road, outside SAC Alert Area, Plattsburgh, Clinton County, NY

  7. 4. WEST REAR ELEVATION OF BUILDING 260 (STORAGE STRUCTURE A) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. WEST REAR ELEVATION OF BUILDING 260 (STORAGE STRUCTURE A) IN STORAGE AREA. - Loring Air Force Base, Weapons Storage Area, Northeastern corner of base at northern end of Maine Road, Limestone, Aroostook County, ME

  8. Oblique view of blacksmith shop, showing north and west (rear) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Oblique view of blacksmith shop, showing north and west (rear) elevations; camera facing southeast - Lemmon-Anderson-Hixson Ranch, Blacksmith Shop, 11220 North Virginia Street, Reno, Washoe County, NV

  9. 5. Interior, third floor rear of 10 East State Street ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Interior, third floor rear of 10 East State Street showing original surviving 6/6 sash window and moldings. - 8-10 East State Street (Commercial Building), 8-10 East State Street, Trenton, Mercer County, NJ

  10. 2. D Street facade and rear (east) blank wall of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. D Street facade and rear (east) blank wall of parking garage. Farther east is 408 8th Street (National Art And Frame Company). - PMI Parking Garage, 403-407 Ninth Street, Northwest, Washington, District of Columbia, DC

  11. 15. City parking lot, and rear of the commercial buildings ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. City parking lot, and rear of the commercial buildings on the east side of State Street. - Lockport Historic District, Bounded by Eighth, Hamilton & Eleventh Streets & Illinois & Michigan Canal, Lockport, Will County, IL

  12. SOUTH (SIDE) AND WEST (REAR) ELEVATIONS OF BUILDING. view TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SOUTH (SIDE) AND WEST (REAR) ELEVATIONS OF BUILDING. view TO NORTH. - Plattsburgh Air Force Base, Target Intelligence Training Building-Combat Center, Off Connecticut Road, east of Idaho Avenue, Plattsburgh, Clinton County, NY

  13. WEST (REAR) AND NORTH (SIDE) ELEVATIONS OF BUILDING. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    WEST (REAR) AND NORTH (SIDE) ELEVATIONS OF BUILDING. VIEW TO EAST. - Plattsburgh Air Force Base, Target Intelligence Training Building-Combat Center, Off Connecticut Road, east of Idaho Avenue, Plattsburgh, Clinton County, NY

  14. 6. MISSILE TEST AND ASSEMBLY BUILDING, REAR AND LEFT SIDES, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. MISSILE TEST AND ASSEMBLY BUILDING, REAR AND LEFT SIDES, LOOKING NORTHWEST. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

  15. 3. MISSILE TEST AND ASSEMBLY BUILDING, REAR SIDE, LOOKING NORTH. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. MISSILE TEST AND ASSEMBLY BUILDING, REAR SIDE, LOOKING NORTH. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

  16. 4. View south of rear of filtration bed building. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. View south of rear of filtration bed building. - Lake Whitney Water Filtration Plant, Filtration Plant, South side of Armory Street between Edgehill Road & Whitney Avenue, Hamden, New Haven County, CT

  17. Rear of REPLACEMENT MODEL single chair from mid station. Tower ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Rear of REPLACEMENT MODEL single chair from mid station. Tower 13 in background, LOOKing northeast. - Mad River Glen, Single Chair Ski Lift, 62 Mad River Glen Resort Road, Fayston, Washington County, VT

  18. VIEW OF EAST SIDE AND NORTH REAR, FACING SOUTHWEST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF EAST SIDE AND NORTH REAR, FACING SOUTHWEST. - Douglas Aircraft Company Long Beach Plant, Aircraft Wing & Fuselage Assembly Building, 3855 Lakewood Boulevard, Long Beach, Los Angeles County, CA

  19. VIEW OF NORTH REAR, BUILDING 13 TO RIGHT, FACING SOUTHWEST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF NORTH REAR, BUILDING 13 TO RIGHT, FACING SOUTHWEST. - Douglas Aircraft Company Long Beach Plant, Aircraft Wing & Fuselage Assembly Building, 3855 Lakewood Boulevard, Long Beach, Los Angeles County, CA

  20. 12. Detail, typical window with fireproof shutters open, northeast rear, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Detail, typical window with fireproof shutters open, northeast rear, view to southwest, 135mm lens. Note cracks evidencing structural failure. - Benicia Arsenal, Powder Magazine No. 5, Junction of Interstate Highways 680 & 780, Benicia, Solano County, CA

  1. 13. Detail, typical window with fireproof shutters closed, northeast rear, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Detail, typical window with fireproof shutters closed, northeast rear, view to southwest, 135mm lens. Note cracks evidencing structural failure. - Benicia Arsenal, Powder Magazine No. 5, Junction of Interstate Highways 680 & 780, Benicia, Solano County, CA

  2. 14. Detail, crack evidencing structural failure, northeast rear, view to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Detail, crack evidencing structural failure, northeast rear, view to southwest, 90mm lens. Note failure of sandstone lintel above window. - Benicia Arsenal, Powder Magazine No. 5, Junction of Interstate Highways 680 & 780, Benicia, Solano County, CA

  3. 15. Detail, cracks evidencing structural failure, northeast rear, view to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Detail, cracks evidencing structural failure, northeast rear, view to southwest, 90mm lens. - Benicia Arsenal, Powder Magazine No. 5, Junction of Interstate Highways 680 & 780, Benicia, Solano County, CA

  4. 3. Rear (north) and east elevations of converted chicken house, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Rear (north) and east elevations of converted chicken house, with smokehouse, cooling (well) house, and residence in background - Henry E. Williams Farmstead, Converted Chicken House, East of Residence & Smokehouse, Cedar Point, Chase County, KS

  5. 6. INTERIOR OF REAR SECTION OF BUILDING 431. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. INTERIOR OF REAR SECTION OF BUILDING 431. VIEW TO NORTHEAST. - Rocky Mountain Arsenal, Ethylene Dryer-Compressor Refrigeration Building, December Seventh Avenue & D Street, Commerce City, Adams County, CO

  6. 11. REAR VIEW, DRAWBRIDGE SIGNAL, EASTBOUND ON CATENARY BRIDGE 522 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. REAR VIEW, DRAWBRIDGE SIGNAL, EASTBOUND ON CATENARY BRIDGE 522 - New York, New Haven & Hartford Railroad, Automatic Signalization System, Long Island Sound shoreline between Stamford & New Haven, Stamford, Fairfield County, CT

  7. 16. Rear (west) side of incinerator. Glove boxes to the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. Rear (west) side of incinerator. Glove boxes to the left. Metal catwalk in the middle. Incinerator control panel to the right. Looking south towards scrubber cell. - Plutonium Finishing Plant, Waste Incinerator Facility, 200 West Area, Richland, Benton County, WA

  8. 17. Rear (west) side of incinerator. Incinerator control panel on ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. Rear (west) side of incinerator. Incinerator control panel on the right. Looking south towards scrubber cell. - Plutonium Finishing Plant, Waste Incinerator Facility, 200 West Area, Richland, Benton County, WA

  9. 5. BARRACKS, NEXT TO BASKETBALL COURT, RIGHT AND REAR SIDES, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. BARRACKS, NEXT TO BASKETBALL COURT, RIGHT AND REAR SIDES, LOOKING EAST. - NIKE Missile Base SL-40, Barracks No. 1, North end of base, southest of Basketball Court & northwest of Barracks No. 2, Hecker, Monroe County, IL

  10. 7. DETAIL INTERIOR VIEW TO SOUTHWEST OF REAR ORIGINAL SECOND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. DETAIL INTERIOR VIEW TO SOUTHWEST OF REAR ORIGINAL SECOND FLOOR, WITH PASSAGEWAY LEADING TO c1944-1950 POST-U.S. RADIUM ADDITION - United States Radium Corporation, Paint Application Building, 422 Alden Street, Orange, Essex County, NJ

  11. 4. VIEW SHOWING SOUTHWEST REAR AND SOUTHEAST SIDE OF POWER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. VIEW SHOWING SOUTHWEST REAR AND SOUTHEAST SIDE OF POWER PLANT/MACHINE SHOP - Clay Spur Bentonite Plant & Camp, Power Plant-Machine Shop, Clay Spur Siding on Burlington Northern Railroad, Osage, Weston County, WY

  12. 2. VIEW SHOWING NORTHWEST SIDE AND SOUTHEAST REAR OF POWER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW SHOWING NORTHWEST SIDE AND SOUTHEAST REAR OF POWER PLANT/MACHINE SHOP - Clay Spur Bentonite Plant & Camp, Power Plant-Machine Shop, Clay Spur Siding on Burlington Northern Railroad, Osage, Weston County, WY

  13. DETAIL OF THE CONCRETE PAVING BLOCKS AT THE REAR OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL OF THE CONCRETE PAVING BLOCKS AT THE REAR OF THE BUILDING. SHOWING THE PIVOTING METAL LOOP RECESSED INTO THE CONCRETE. VIEW FACING EAST. - Hickam Field, Officers' Housing Type A, 601 Boquet Boulevard, Honolulu, Honolulu County, HI

  14. FIRST FLOOR REAR ROOM. SECOND FLOOR HAS BEEN REMOVED NOTE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    FIRST FLOOR REAR ROOM. SECOND FLOOR HAS BEEN REMOVED-- NOTE PRESENCE OF SECOND FLOOR WINDOWS AT LEFT. See also PA-1436 B-13 - Kid-Physick House, 325 Walnut Street, Philadelphia, Philadelphia County, PA

  15. FIRST FLOOR REAR ROOM. SECOND FLOOR HAS BEEN REMOVED NOTE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    FIRST FLOOR REAR ROOM. SECOND FLOOR HAS BEEN REMOVED-- NOTE PRESENCE OF SECOND FLOOR WINDOWS AT LEFT. See also PA-1436 B-6 - Kid-Physick House, 325 Walnut Street, Philadelphia, Philadelphia County, PA

  16. 2. View facing east showing the south elevation (rear) of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. View facing east showing the south elevation (rear) of the garage, loading docks and rail spur, with Providence Fruit & Produce Building beyond. - Armour & Company Building, 100 Harris Avenue, Providence, Providence County, RI

  17. 4. West rear and south side of building. View to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. West rear and south side of building. View to northeast. - U.S. Customs Service Port of Roosville, Immigration & Naturalization Service Residence, 45 feet southwest of Main Port Building, Eureka, Lincoln County, MT

  18. 35. REAR OF SWITCHBOARD, OLDER ARRANGEMENT. SCE negative no. 10328, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    35. REAR OF SWITCHBOARD, OLDER ARRANGEMENT. SCE negative no. 10328, November 1, 1923. Photograph by G. Haven Bishop. - Santa Ana River Hydroelectric System, SAR-2 Powerhouse, Redlands, San Bernardino County, CA

  19. 36. REAR OF SWITCHBOARD, NEWLY MODIFIED. SCE negative no. 15349, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    36. REAR OF SWITCHBOARD, NEWLY MODIFIED. SCE negative no. 15349, May 21, 1928. Photograph by G. Haven Bishop. - Santa Ana River Hydroelectric System, SAR-2 Powerhouse, Redlands, San Bernardino County, CA

  20. South (side) and east (rear) elevations, view to northwest ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South (side) and east (rear) elevations, view to northwest - Bureau of Mines Boulder City Experimental Station, Titanium Development Plant, Date Street north of U.S. Highway 93, Boulder City, Clark County, NV